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Title: Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization

Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [5] ;  [1] ;  [6] ;  [1] ;  [1] ;  [3]
  1. Univ. of Brest, Brest Cedex (France)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Research and Development Luminy Biotech Enterprises, Marseille (France)
  3. Univ. of Brest, Brest Cedex (France); Univ. Hospital of Brest, Brest (France)
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  5. Univ. Hospital of Brest, Brest (France)
  6. CF Center, Roscoff (France)
Publication Date:
OSTI Identifier:
1212084
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
SpringerPlus
Additional Journal Information:
Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2193-1801
Publisher:
Springer
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE; French Cystic Fibrosis Association: 'Vaincre la Mucoviscidose'; French Ministry of Higher Education and Research (Ministère de l’Enseignement Supérieur et de la Recherche: MESR, France)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES cystic fibrosis; respiratory tract microbiota; Pseudomonas aeruginosa; early colonization; anaerobes; respiratory viruses