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Title: Crystal structure of a two-subunit TrkA octameric gating ring assembly

The TM1088 locus of T. maritima codes for two proteins designated TM1088A and TM1088B, which combine to form the cytosolic portion of a putative Trk K⁺ transporter. We report the crystal structure of this assembly to a resolution of 3.45 Å. The high resolution crystal structures of the components of the assembly, TM1088A and TM1088B, were also determined independently to 1.50 Å and 1.55 Å, respectively. The TM1088 proteins are structurally homologous to each other and to other K⁺ transporter proteins, such as TrkA. These proteins form a cytosolic gating ring assembly that controls the flow of K⁺ ions across the membrane. TM1088 represents the first structure of a two-subunit Trk assembly. Despite the atypical genetics and chain organization of the TM1088 assembly, it shares significant structural homology and an overall quaternary organization with other single-subunit K⁺ gating ring assemblies. This structure provides the first structural insights into what may be an evolutionary ancestor of more modern single-subunit K⁺ gating ring assemblies.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [1] ;  [5] ;  [6]
  1. Scripps Research Inst., La Jolla, CA (United States). The Joint Center for Structural Genomics, and Dept. of Integrative Structural and Computional Biology.
  2. Scripps Research Inst., La Jolla, CA (United States). The Joint Center for Structural Genomics, and Dept. of Integrative Structural and Computional Biology; California State Univ., Fullerton, CA (United States)
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States). The Joint Center for Structural Genomics, and Stanford Synchrotron Radiation Lightsource; Rice Univ., Houston, TX (United States)
  4. Genomics Inst. of the Novartis Research Foundation, San Diego, CA (United States). Protein Sciences Dept.
  5. Scripps Research Inst., La Jolla, CA (United States). The Joint Center for Structural Genomics, and Dept. of Integrative Structural and Computional Biology; Genomics Inst. of the Novartis Research Foundation, San Diego, CA (United States). Protein Sciences Dept.
  6. Zhejiang Univ., Hangzhou (China)
Publication Date:
OSTI Identifier:
1211628
Grant/Contract Number:
AC03-76SF00098; U54 GM094586
Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 10; Journal Issue: 3; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Protein Structure Initiative of the National Institute of General Medical Sciences, National institutes of Health (NIH)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES