Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases
Abstract
Zinc finger nucleases (ZFNs) are associated with cell death and apoptosis by binding at countless undesired locations. This cytotoxicity is associated with the binding ability of engineered zinc finger domains to bind dissimilar DNA sequences with high affinity. In general, binding preferences of transcription factors are associated with significant degenerated diversity and complexity which convolutes the design and engineering of precise DNA binding domains. Evolutionary success of natural zinc finger proteins, however, evinces that nature created specific evolutionary traits and strategies, such as modularity and rank-specific recognition to cope with binding complexity that are critical for creating clinical viable tools to precisely modify the human genome. Our findings indicate preservation of general modularity and significant alteration of the rank-specific binding preferences of the three-finger binding domain of transcription factor SP1 when exchanging amino acids in the 2nd finger.
- Authors:
-
- University of Bridgeport, Biomedical Engineering, 221 University Avenue, Bridgeport, CT 06604, USA
- Physics Faculty, BHSEC Queens, 30-20 Thomson Avenue, Long Island City, NY 11101, USA
- University of Bridgeport, Mechanical Engineering, 221 University Avenue, Bridgeport, CT 06604, USA
- University of Bridgeport, Electrical Engineering, 221 University Avenue, Bridgeport, CT 06604, USA
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1209764
- Alternate Identifier(s):
- OSTI ID: 1629932
- Grant/Contract Number:
- AC02-98CH10886
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology Research International
- Additional Journal Information:
- Journal Name: Biotechnology Research International Journal Volume: 2014; Journal ID: ISSN 2090-3138
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- Egypt
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 97 MATHEMATICS AND COMPUTING
Citation Formats
Bach, Christian, Sherman, William, Pallis, Jani, Patra, Prabir, and Bajwa, Hassan. Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases. Egypt: N. p., 2014.
Web. doi:10.1155/2014/970595.
Bach, Christian, Sherman, William, Pallis, Jani, Patra, Prabir, & Bajwa, Hassan. Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases. Egypt. https://doi.org/10.1155/2014/970595
Bach, Christian, Sherman, William, Pallis, Jani, Patra, Prabir, and Bajwa, Hassan. Sun .
"Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases". Egypt. https://doi.org/10.1155/2014/970595.
@article{osti_1209764,
title = {Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases},
author = {Bach, Christian and Sherman, William and Pallis, Jani and Patra, Prabir and Bajwa, Hassan},
abstractNote = {Zinc finger nucleases (ZFNs) are associated with cell death and apoptosis by binding at countless undesired locations. This cytotoxicity is associated with the binding ability of engineered zinc finger domains to bind dissimilar DNA sequences with high affinity. In general, binding preferences of transcription factors are associated with significant degenerated diversity and complexity which convolutes the design and engineering of precise DNA binding domains. Evolutionary success of natural zinc finger proteins, however, evinces that nature created specific evolutionary traits and strategies, such as modularity and rank-specific recognition to cope with binding complexity that are critical for creating clinical viable tools to precisely modify the human genome. Our findings indicate preservation of general modularity and significant alteration of the rank-specific binding preferences of the three-finger binding domain of transcription factor SP1 when exchanging amino acids in the 2nd finger.},
doi = {10.1155/2014/970595},
journal = {Biotechnology Research International},
number = ,
volume = 2014,
place = {Egypt},
year = {Sun Apr 06 00:00:00 EDT 2014},
month = {Sun Apr 06 00:00:00 EDT 2014}
}
https://doi.org/10.1155/2014/970595
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