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Title: Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation

Abstract

Long noncoding RNAs (lncRNAs) are emerging as key regulators of diverse cell functions and processes. However, the relevance of lncRNAs in the cell and tissue response to ionizing radiation has not yet been characterized. Here we used microarray profiling to determine lncRNA and mRNA expression in mammary glands of BALB/c and SPRET/EiJ mice after low-dose ionizing radiation (LDIR) exposure. We found that unirradiated mammary tissues of these strains differed significantly in baseline expressions of 290 lncRNAs. LDIR exposure (10 cGy) induced a significant change in the expression of many lncRNAs. The vast majority of lncRNAs identified to be differentially expressed after LDIR in either BALB/c or SPRET/EiJ had a significantly correlated expression pattern with at least one LDIR responsive mRNA. Functional analysis revealed that the response to LDIR in BALB/c mice is highly dynamic with enrichment for genes involved in tissue injury, inflammatory responses, and mammary gland development at 2, 4, and 8 weeks after LDIR, respectively. Our study demonstrates that genetic background strongly influences the expression of lncRNAs and their response to radiation and that lncRNAs may coordinate the tissue response to LDIR exposure via regulation of coding mRNAs.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
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  1. Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road MS977, Berkeley, CA 94720, USA
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1227911
Alternate Identifier(s):
OSTI ID: 1209505; OSTI ID: 1512170
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
International Journal of Genomics
Additional Journal Information:
Journal Name: International Journal of Genomics Journal Volume: 2015; Journal ID: ISSN 2314-436X
Publisher:
Hindawi Publishing Corporation
Country of Publication:
Egypt
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.

Citation Formats

Tang, Jonathan, Huang, Yurong, Nguyen, David H., Costes, Sylvain V., Snijders, Antoine M., and Mao, Jian-Hua. Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation. Egypt: N. p., 2015. Web. doi:10.1155/2015/461038.
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Tang, Jonathan, Huang, Yurong, Nguyen, David H., Costes, Sylvain V., Snijders, Antoine M., & Mao, Jian-Hua. Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation. Egypt. https://doi.org/10.1155/2015/461038
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Tang, Jonathan, Huang, Yurong, Nguyen, David H., Costes, Sylvain V., Snijders, Antoine M., and Mao, Jian-Hua. Thu . "Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation". Egypt. https://doi.org/10.1155/2015/461038.
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@article{osti_1227911,
title = {Genetic Background Modulates lncRNA-Coordinated Tissue Response to Low Dose Ionizing Radiation},
author = {Tang, Jonathan and Huang, Yurong and Nguyen, David H. and Costes, Sylvain V. and Snijders, Antoine M. and Mao, Jian-Hua},
abstractNote = {Long noncoding RNAs (lncRNAs) are emerging as key regulators of diverse cell functions and processes. However, the relevance of lncRNAs in the cell and tissue response to ionizing radiation has not yet been characterized. Here we used microarray profiling to determine lncRNA and mRNA expression in mammary glands of BALB/c and SPRET/EiJ mice after low-dose ionizing radiation (LDIR) exposure. We found that unirradiated mammary tissues of these strains differed significantly in baseline expressions of 290 lncRNAs. LDIR exposure (10 cGy) induced a significant change in the expression of many lncRNAs. The vast majority of lncRNAs identified to be differentially expressed after LDIR in either BALB/c or SPRET/EiJ had a significantly correlated expression pattern with at least one LDIR responsive mRNA. Functional analysis revealed that the response to LDIR in BALB/c mice is highly dynamic with enrichment for genes involved in tissue injury, inflammatory responses, and mammary gland development at 2, 4, and 8 weeks after LDIR, respectively. Our study demonstrates that genetic background strongly influences the expression of lncRNAs and their response to radiation and that lncRNAs may coordinate the tissue response to LDIR exposure via regulation of coding mRNAs.},
doi = {10.1155/2015/461038},
journal = {International Journal of Genomics},
number = ,
volume = 2015,
place = {Egypt},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1155/2015/461038
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Cited by: 6 works
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Figures / Tables:

Figure 1 Figure 1: Significant strain differences in lncRNA and coding RNA expression in mammary tissues of BALB/c and SPRET/EiJ mice. (a)-(b) Hierarchical clustering of baseline differences in lncRNA (a) and coding RNA (b) expression in mammary gland tissues of 8-9-week-old BALB/c and SPRET/EiJ mice (fold-change 1.5; 𝑝 value < 0.001). Increasedmore » expression indicated in red and decreased expression in green. (c) Gene interaction networks of genes expressed at lower levels in BALB/c mammary tissues when compared to SPRET/EiJ (top) and genes expressed at higher levels in BALB/c compared to SPRET/EiJ (bottom) (see Figure S3 for annotation of molecular shapes used in the networks).« less
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