Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium
Abstract
Objective: The objective of this study was to investigate the effect of mechanical strain by mapping physicochemical properties at periodontal ligament (PDL)–bone and PDL–cementum attachment sites and within the tissues per se. Design: Accentuated mechanical strain was induced by applying a unidirectional force of 0.06 N for 14 days on molars in a rat model. The associated changes in functional space between the tooth and bone, mineral forming and resorbing events at the PDL–bone and PDL–cementum attachment sites were identified by using micro-X-ray computed tomography (micro-XCT), atomic force microscopy (AFM), dynamic histomorphometry, Raman microspectroscopy, and AFM-based nanoindentation technique. Results from these analytical techniques were correlated with histochemical strains specific to low and high molecular weight GAGs, including biglycan, and osteoclast distribution through tartrate resistant acid phosphatase (TRAP) staining. Results: Unique chemical and mechanical qualities including heterogeneous bony fingers with hygroscopic Sharpey's fibers contributing to a higher organic (amide III — 1240 cm⁻¹) to inorganic (phosphate — 960 cm⁻¹) ratio, with lower average elastic modulus of 8 GPa versus 12 GPa in unadapted regions were identified. Furthermore, an increased presence of elemental Zn in cement lines and mineralizing fronts of PDL–bone was observed. Adapted regions containing bony fingers exhibited woven bone-likemore »
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1251867
- Alternate Identifier(s):
- OSTI ID: 1208648; OSTI ID: 1215419
- Grant/Contract Number:
- AC02-76SF00515; AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Bone Reports
- Additional Journal Information:
- Journal Name: Bone Reports Journal Volume: 3 Journal Issue: C; Journal ID: ISSN 2352-1872
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; mineralization; adaptations; bone–periodontal ligament–tooth complex; mechanical strain; interfaces; attachment sites
Citation Formats
Grandfield, Kathryn, Herber, Ralf-Peter, Chen, Ling, Djomehri, Sabra, Tam, Caleb, Lee, Ji-Hyun, Brown, Evan, Woolwine, III, Wood R., Curtis, Don, Ryder, Mark, Schuck, Jim, Webb, Samuel, Landis, William, and Ho, Sunita P. Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium. United States: N. p., 2015.
Web. doi:10.1016/j.bonr.2015.04.002.
Grandfield, Kathryn, Herber, Ralf-Peter, Chen, Ling, Djomehri, Sabra, Tam, Caleb, Lee, Ji-Hyun, Brown, Evan, Woolwine, III, Wood R., Curtis, Don, Ryder, Mark, Schuck, Jim, Webb, Samuel, Landis, William, & Ho, Sunita P. Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium. United States. https://doi.org/10.1016/j.bonr.2015.04.002
Grandfield, Kathryn, Herber, Ralf-Peter, Chen, Ling, Djomehri, Sabra, Tam, Caleb, Lee, Ji-Hyun, Brown, Evan, Woolwine, III, Wood R., Curtis, Don, Ryder, Mark, Schuck, Jim, Webb, Samuel, Landis, William, and Ho, Sunita P. Tue .
"Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium". United States. https://doi.org/10.1016/j.bonr.2015.04.002.
@article{osti_1251867,
title = {Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium},
author = {Grandfield, Kathryn and Herber, Ralf-Peter and Chen, Ling and Djomehri, Sabra and Tam, Caleb and Lee, Ji-Hyun and Brown, Evan and Woolwine, III, Wood R. and Curtis, Don and Ryder, Mark and Schuck, Jim and Webb, Samuel and Landis, William and Ho, Sunita P.},
abstractNote = {Objective: The objective of this study was to investigate the effect of mechanical strain by mapping physicochemical properties at periodontal ligament (PDL)–bone and PDL–cementum attachment sites and within the tissues per se. Design: Accentuated mechanical strain was induced by applying a unidirectional force of 0.06 N for 14 days on molars in a rat model. The associated changes in functional space between the tooth and bone, mineral forming and resorbing events at the PDL–bone and PDL–cementum attachment sites were identified by using micro-X-ray computed tomography (micro-XCT), atomic force microscopy (AFM), dynamic histomorphometry, Raman microspectroscopy, and AFM-based nanoindentation technique. Results from these analytical techniques were correlated with histochemical strains specific to low and high molecular weight GAGs, including biglycan, and osteoclast distribution through tartrate resistant acid phosphatase (TRAP) staining. Results: Unique chemical and mechanical qualities including heterogeneous bony fingers with hygroscopic Sharpey's fibers contributing to a higher organic (amide III — 1240 cm⁻¹) to inorganic (phosphate — 960 cm⁻¹) ratio, with lower average elastic modulus of 8 GPa versus 12 GPa in unadapted regions were identified. Furthermore, an increased presence of elemental Zn in cement lines and mineralizing fronts of PDL–bone was observed. Adapted regions containing bony fingers exhibited woven bone-like architecture and these regions rich in biglycan (BGN) and bone sialoprotein (BSP) also contained high-molecular weight polysaccharides predominantly at the site of polarized bone growth. Conclusions: From a fundamental science perspective the shift in local properties due to strain amplification at the soft–hard tissue attachment sites is governed by semiautonomous cellular events at the PDL–bone and PDL–cementum sites. Over time, these strain-mediated events can alter the physicochemical properties of tissues per se, and consequently the overall biomechanics of the bone–PDL–tooth complex. From a clinical perspective, the shifts in magnitude and duration of forces on the periodontal ligament can prompt a shift in physiologic mineral apposition in cementum and alveolar bone albeit of an adapted quality owing to the rapid mechanical translation of the tooth.},
doi = {10.1016/j.bonr.2015.04.002},
journal = {Bone Reports},
number = C,
volume = 3,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 2015},
month = {Tue Dec 01 00:00:00 EST 2015}
}
https://doi.org/10.1016/j.bonr.2015.04.002
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