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Title: Structure and Dynamics of Dinucleosomes Assessed by Atomic Force Microscopy

Dynamics of nucleosomes and their interactions are important for understanding the mechanism of chromatin assembly. Internucleosomal interaction is required for the formation of higher-order chromatin structures. Although H1 histone is critically involved in the process of chromatin assembly, direct internucleosomal interactions contribute to this process as well. To characterize the interactions of nucleosomes within the nucleosome array, we designed a dinucleosome and performed direct AFM imaging. The analysis of the AFM data showed dinucleosomes are very dynamic systems, enabling the nucleosomes to move in a broad range along the DNA template. Di-nucleosomes in close proximity were observed, but their population was low. The use of the zwitterionic detergent, CHAPS, increased the dynamic range of the di-nucleosome, facilitating the formation of tight di-nucleosomes. The role of CHAPS and similar natural products in chromatin structure and dynamics is also discussed.
Authors:
 [1] ;  [2] ;  [3]
  1. Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA, Department of High Technologies, The International Solomon University, 1b Sholudenko Street, Kyiv 01135, Ukraine
  2. Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA, Department of Application Services, Infopulse Ukraine LLC, 24 Polyova Street, Kyiv 03056, Ukraine
  3. Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA
Publication Date:
OSTI Identifier:
1198215
Grant/Contract Number:
FG02-08ER64579
Type:
Published Article
Journal Name:
Journal of Amino Acids
Additional Journal Information:
Journal Volume: 2012; Related Information: CHORUS Timestamp: 2016-08-02 18:34:05; Journal ID: ISSN 2090-0104
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English