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Title: Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy

Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05-0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolism in living human cells, and produces only minimal sample heating (<0.5°C). We will then present several examples demonstrating the application potentials of SR-FTIR spectromicroscopy in biomedical research. These will include monitoring living cells progressing through the cell cycle, including death, and cells reacting to dilute concentrations of toxins.
Authors:
; ;
Publication Date:
OSTI Identifier:
1198202
Grant/Contract Number:
AC03-76SF00098
Type:
Published Article
Journal Name:
Spectroscopy
Additional Journal Information:
Journal Volume: 17; Journal Issue: 2-3; Related Information: CHORUS Timestamp: 2016-08-23 05:27:10; Journal ID: ISSN 0712-4813
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English