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Title: Bioassays Based on Molecular Nanomechanics

Recent experiments have shown that when specific biomolecular interactions are confined to one surface of a microcantilever beam, changes in intermolecular nanomechanical forces provide sufficient differential torque to bend the cantilever beam. This has been used to detect single base pair mismatches during DNA hybridization, as well as prostate specific antigen (PSA) at concentrations and conditions that are clinically relevant for prostate cancer diagnosis. Since cantilever motion originates from free energy change induced by specific biomolecular binding, this technique is now offering a common platform for label-free quantitative analysis of protein-protein binding, DNA hybridization DNA-protein interactions, and in general receptor-ligand interactions. Current work is focused on developing “universal microarrays” of microcantilever beams for high-throughput multiplexed bioassays.
Authors:
 [1]
  1. Nanoengineering Laboratory, Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA
Publication Date:
OSTI Identifier:
1198194
Type:
Published Article
Journal Name:
Disease Markers
Additional Journal Information:
Journal Volume: 18; Journal Issue: 4; Related Information: CHORUS Timestamp: 2016-08-23 11:13:39; Journal ID: ISSN 0278-0240
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English