Bioassays Based on Molecular Nanomechanics
Abstract
Recent experiments have shown that when specific biomolecular interactions are confined to one surface of a microcantilever beam, changes in intermolecular nanomechanical forces provide sufficient differential torque to bend the cantilever beam. This has been used to detect single base pair mismatches during DNA hybridization, as well as prostate specific antigen (PSA) at concentrations and conditions that are clinically relevant for prostate cancer diagnosis. Since cantilever motion originates from free energy change induced by specific biomolecular binding, this technique is now offering a common platform for label-free quantitative analysis of protein-protein binding, DNA hybridization DNA-protein interactions, and in general receptor-ligand interactions. Current work is focused on developing “universal microarrays” of microcantilever beams for high-throughput multiplexed bioassays.
- Authors:
-
- Nanoengineering Laboratory, Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1198194
- Resource Type:
- Published Article
- Journal Name:
- Disease Markers
- Additional Journal Information:
- Journal Name: Disease Markers Journal Volume: 18 Journal Issue: 4; Journal ID: ISSN 0278-0240
- Publisher:
- Hindawi Publishing Corporation
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Majumdar, Arun. Bioassays Based on Molecular Nanomechanics. Country unknown/Code not available: N. p., 2002.
Web. doi:10.1155/2002/856032.
Majumdar, Arun. Bioassays Based on Molecular Nanomechanics. Country unknown/Code not available. https://doi.org/10.1155/2002/856032
Majumdar, Arun. Tue .
"Bioassays Based on Molecular Nanomechanics". Country unknown/Code not available. https://doi.org/10.1155/2002/856032.
@article{osti_1198194,
title = {Bioassays Based on Molecular Nanomechanics},
author = {Majumdar, Arun},
abstractNote = {Recent experiments have shown that when specific biomolecular interactions are confined to one surface of a microcantilever beam, changes in intermolecular nanomechanical forces provide sufficient differential torque to bend the cantilever beam. This has been used to detect single base pair mismatches during DNA hybridization, as well as prostate specific antigen (PSA) at concentrations and conditions that are clinically relevant for prostate cancer diagnosis. Since cantilever motion originates from free energy change induced by specific biomolecular binding, this technique is now offering a common platform for label-free quantitative analysis of protein-protein binding, DNA hybridization DNA-protein interactions, and in general receptor-ligand interactions. Current work is focused on developing “universal microarrays” of microcantilever beams for high-throughput multiplexed bioassays.},
doi = {10.1155/2002/856032},
journal = {Disease Markers},
number = 4,
volume = 18,
place = {Country unknown/Code not available},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
https://doi.org/10.1155/2002/856032
Web of Science