Gel Electrophoresis of Gold-DNA Nanoconjugates

Pellegrino, T.; Sperling, R. A.; Alivisatos, A. P.; Parak, W. J.

doi:10.1155/2007/26796

Title: Gel Electrophoresis of Gold-DNA Nanoconjugates

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Abstract

Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifically via thiol-Au bonds or by nonspecific adsorption. From the experimentally determined electrophoretic mobilities, estimates for the effective diameters of the gold-DNA conjugates were derived by applying two different data treatment approaches. The first method is based on making a calibration curve for the relation between effective diameters and mobilities with gold nanoparticles of known diameter. The second method is based on Ferguson analysis which uses gold nanoparticles of known diameter as reference database. Our study shows that effective diameters derived from gel electrophoresis measurements are affected with a high error bar as the determined values strongly depend on the method of evaluation, though relative changes in size upon binding of molecules can be detected with high precision. Furthermore, in this study, the specific attachment of DNA via gold-thiol bonds to Au nanoparticles is compared to nonspecific adsorption of DNA. Also, the maximum number of DNA moleculesmore »« less

Authors:: Pellegrino, T.; Sperling, R. A.; Alivisatos, A. P.; Parak, W. J.

Publication Date:: Mon Jan 01 00:00:00 EST 2007

Sponsoring Org.:: USDOE

OSTI Identifier:: 1198185

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Journal of Biomedicine and Biotechnology

Additional Journal Information:: Journal Name: Journal of Biomedicine and Biotechnology Journal Volume: 2007; Journal ID: ISSN 1110-7243

Publisher:: Hindawi Publishing Corporation

Country of Publication:: Country unknown/Code not available

Language:: English

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                    Pellegrino, T., Sperling, R. A., Alivisatos, A. P., and Parak, W. J. Gel Electrophoresis of Gold-DNA Nanoconjugates.  Country unknown/Code not available: N. p., 2007. 
Web.  doi:10.1155/2007/26796.

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                    Pellegrino, T., Sperling, R. A., Alivisatos, A. P., & Parak, W. J. Gel Electrophoresis of Gold-DNA Nanoconjugates.  Country unknown/Code not available.  https://doi.org/10.1155/2007/26796

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                    Pellegrino, T., Sperling, R. A., Alivisatos, A. P., and Parak, W. J. Mon .  
"Gel Electrophoresis of Gold-DNA Nanoconjugates".  Country unknown/Code not available.  https://doi.org/10.1155/2007/26796.

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@article{osti_1198185,

  title        = {Gel Electrophoresis of Gold-DNA Nanoconjugates},

  author       = {Pellegrino, T. and Sperling, R. A. and Alivisatos, A. P. and Parak, W. J.},

  abstractNote = {Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifically via thiol-Au bonds or by nonspecific adsorption. From the experimentally determined electrophoretic mobilities, estimates for the effective diameters of the gold-DNA conjugates were derived by applying two different data treatment approaches. The first method is based on making a calibration curve for the relation between effective diameters and mobilities with gold nanoparticles of known diameter. The second method is based on Ferguson analysis which uses gold nanoparticles of known diameter as reference database. Our study shows that effective diameters derived from gel electrophoresis measurements are affected with a high error bar as the determined values strongly depend on the method of evaluation, though relative changes in size upon binding of molecules can be detected with high precision. Furthermore, in this study, the specific attachment of DNA via gold-thiol bonds to Au nanoparticles is compared to nonspecific adsorption of DNA. Also, the maximum number of DNA molecules that can be bound per particle was determined.},

  doi          = {10.1155/2007/26796},

  journal      = {Journal of Biomedicine and Biotechnology},

  number       = ,

  volume       = 2007,

  place        = {Country unknown/Code not available},

  year         = {Mon Jan 01 00:00:00 EST 2007},

  month        = {Mon Jan 01 00:00:00 EST 2007}

}

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