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Title: Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [3] ;  [3] ;  [1]
  1. The Center for Mass Spectrometry and Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA-92037, USA
  2. Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA-92037, USA
  3. Becton Dickinson, Sparks, MD-21152, USA
Publication Date:
OSTI Identifier:
1198015
Grant/Contract Number:
AC02-05CH1123
Type:
Published Article
Journal Name:
Spectroscopy
Additional Journal Information:
Journal Volume: 22; Journal Issue: 5; Related Information: CHORUS Timestamp: 2016-08-23 10:00:07; Journal ID: ISSN 0712-4813
Publisher:
Hindawi Publishing Corporation
Sponsoring Org:
USDOE
Country of Publication:
Country unknown/Code not available
Language:
English