Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods

Shemilt, Laura; Verbanis, Ephanielle; Schwenke, Joerg; Estandarte, Ana K.; Xiong, Gang; Harder, Ross; Parmar, Neha; Yusuf, Mohammed; Zhang, Fucai; Robinson, Ian K.

doi:10.1016/j.bpj.2014.11.3456

Title: Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods

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Abstract

Sorting and identifying chromosomes, a process known as karyotyping, is widely used to detect changes in chromosome shapes and gene positions. In a karyotype the chromosomes are identified by their size and therefore this process can be performed by measuring macroscopic structural variables. Chromosomes contain a specific number of basepairs that linearly correlate with their size; therefore, it is possible to perform a karyotype on chromosomes using their mass as an identifying factor. Here, we obtain the first images, to our knowledge, of chromosomes using the novel imaging method of ptychography. We can use the images to measure the mass of chromosomes and perform a partial karyotype from the results. Lastly, we also obtain high spatial resolution using this technique with synchrotron source x-rays.

Authors:: Shemilt, Laura; Verbanis, Ephanielle; Schwenke, Joerg; Estandarte, Ana K.; Xiong, Gang; Harder, Ross; Parmar, Neha; Yusuf, Mohammed; Zhang, Fucai; Robinson, Ian K.

Publication Date:: Sun Feb 01 00:00:00 EST 2015

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source.; Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1233919

Alternate Identifier(s):: OSTI ID: 1196335; OSTI ID: 1249549

Grant/Contract Number:: AC02-06CH11357

Resource Type:: Published Article

Journal Name:: Biophysical Journal

Additional Journal Information:: Journal Name: Biophysical Journal Journal Volume: 108 Journal Issue: 3; Journal ID: ISSN 0006-3495

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Shemilt, Laura, Verbanis, Ephanielle, Schwenke, Joerg, Estandarte, Ana K., Xiong, Gang, Harder, Ross, Parmar, Neha, Yusuf, Mohammed, Zhang, Fucai, and Robinson, Ian K. Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods.  United States: N. p., 2015. 
Web.  doi:10.1016/j.bpj.2014.11.3456.

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                    Shemilt, Laura, Verbanis, Ephanielle, Schwenke, Joerg, Estandarte, Ana K., Xiong, Gang, Harder, Ross, Parmar, Neha, Yusuf, Mohammed, Zhang, Fucai, & Robinson, Ian K. Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods.  United States.  https://doi.org/10.1016/j.bpj.2014.11.3456

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                    Shemilt, Laura, Verbanis, Ephanielle, Schwenke, Joerg, Estandarte, Ana K., Xiong, Gang, Harder, Ross, Parmar, Neha, Yusuf, Mohammed, Zhang, Fucai, and Robinson, Ian K. Sun .  
"Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods".  United States.  https://doi.org/10.1016/j.bpj.2014.11.3456.

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@article{osti_1233919,

  title        = {Karyotyping Human Chromosomes by Optical and X-Ray Ptychography Methods},

  author       = {Shemilt, Laura and Verbanis, Ephanielle and Schwenke, Joerg and Estandarte, Ana K. and Xiong, Gang and Harder, Ross and Parmar, Neha and Yusuf, Mohammed and Zhang, Fucai and Robinson, Ian K.},

  abstractNote = {Sorting and identifying chromosomes, a process known as karyotyping, is widely used to detect changes in chromosome shapes and gene positions. In a karyotype the chromosomes are identified by their size and therefore this process can be performed by measuring macroscopic structural variables. Chromosomes contain a specific number of basepairs that linearly correlate with their size; therefore, it is possible to perform a karyotype on chromosomes using their mass as an identifying factor. Here, we obtain the first images, to our knowledge, of chromosomes using the novel imaging method of ptychography. We can use the images to measure the mass of chromosomes and perform a partial karyotype from the results. Lastly, we also obtain high spatial resolution using this technique with synchrotron source x-rays.},

  doi          = {10.1016/j.bpj.2014.11.3456},

  journal      = {Biophysical Journal},

  number       = 3,

  volume       = 108,

  place        = {United States},

  year         = {Sun Feb 01 00:00:00 EST 2015},

  month        = {Sun Feb 01 00:00:00 EST 2015}

}

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