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Title: Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [5] ;  [5] ;  [6] ;  [5]
  1. Yeshiva Univ., New York, NY (United States); Stanford Univ., Stanford, CA (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States)
  3. Yeshiva Univ., New York, NY (United States); Univ. of Pennsylvania, Philadelphia, PA (United States)
  4. Yeshiva Univ., New York, NY (United States); Yeshiva Univ., Bronx, NY (United States)
  5. Yeshiva Univ., New York, NY (United States)
  6. Collegiate School, New York, NY (United States)
Publication Date:
OSTI Identifier:
1193215
Report Number(s):
BNL--108006-2015-JA
Journal ID: ISSN 1399-0047; ABCRE6; R&D Project: LS001
Grant/Contract Number:
SC00112704
Type:
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section D: Biological Crystallography (Online)
Additional Journal Information:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography (Online); Journal Volume: 71; Journal Issue: 3; Journal ID: ISSN 1399-0047
Publisher:
International Union of Crystallography
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE MPD; crystallization additive; precipitant; high-resolution protein structures; chirality