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Title: Liquid microjunction surface sampling of acetaminophen, terfenadine and their metabolites in thin tissue sections

The aim of this paper was to evaluate the analytical performance of a fully automated droplet-based surface-sampling system for determining the distribution of the drugs acetaminophen and terfenadine, and their metabolites, in rat thin tissue sections. The following are the results: The rank order of acetaminophen concentration observed in tissues was stomach > small intestine > liver, while the concentrations of its glucuronide and sulfate metabolites were greatest in the liver and small intestine. Terfenadine was most concentrated in the liver and kidney, while its major metabolite, fexofenadine, was found in the liver and small intestine. In conclusion, the spatial distributions of both drugs and their respective metabolites observed in this work were consistent with previous studies using radiolabeled drugs.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Organic and Biological Mass Spectrometry Group. Chemical Sciences Division
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Organic and Biological Mass Spectrometry Group. Chemical Sciences Division; Georgia Inst. of Technology, Atlanta, GA (United States). ORISE HERE Intern
  3. Novartis Inst. for Biomedical Research (NIBR), East Hanover, NJ (United States)
Publication Date:
OSTI Identifier:
1185413
Grant/Contract Number:
AC05-00OR22725; NFE-10-02966
Type:
Accepted Manuscript
Journal Name:
Bioanalysis
Additional Journal Information:
Journal Volume: 6; Journal Issue: 19; Journal ID: ISSN 1757-6180
Publisher:
Future Science Group
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE; AB Sciex, Framingham, MA (United States); Novartis Inst. for BioMedical Research (NIBR), Inc., Cambridge, MA (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES liquid microjunction; droplet-based liquid extraction; autosampler; drug discovery; spatial distribution