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Title: Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus

In this article, we report here the sequencing and analysis of the genome of the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS. This microbe is a model for studies of its carboxydotrophic life style under anaerobic condition, based on its ability to utilize carbon monoxide (CO) as the sole carbon substrate and water as the electron acceptor, yielding CO2 and H2 as the end products. The CO-oxidation reaction is known to be catalyzed by two enzyme complexes, the CO dehydrogenase and hydrogenase. As expected, analysis of the genome of Rx. gelatinosus CBS reveals the presence of genes encoding both enzyme complexes. The CO-oxidation reaction is CO-inducible, which is consistent with the presence of two putative CO-sensing transcription factors in its genome. Genome analysis also reveals the presence of two additional hydrogenases, an uptake hydrogenase that liberates the electrons in H2 in support of cell growth, and a regulatory hydrogenase that senses H2 and relays the signal to a two-component system that ultimately controls synthesis of the uptake hydrogenase. The genome also contains two sets of hydrogenase maturation genes which are known to assemble the catalytic metallocluster of the hydrogenase NiFe active site. Finally and collectively, the genome sequence and analysismore » information reveals the blueprint of an intricate network of signal transduction pathways and its underlying regulation that enables Rx. gelatinosus CBS to thrive on CO or H2 in support of cell growth.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [1] ;  [1]
  1. Univ. of Wyoming, Laramie, WY (United States). Dept. of Chemical and Petroleum Engineering
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
  3. Pacific Biosciences, Menlo Park, CA (United States
  4. Michigan State Univ., East Lansing, MI (United States). Dept. of Energy Plant Research Lab.
Publication Date:
OSTI Identifier:
1169210
Report Number(s):
NREL/JA--2700-61988
Journal ID: ISSN 1932-6203
Grant/Contract Number:
FG02-91ER20021
Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 9; Journal Issue: 12; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Fuel Cell Technologies Program (EE-3F);USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 08 HYDROGEN chemical and biosciences; hydrogenase; CO dehydrogenase; photosynthetic bacteria; Rubrivivax gelatinosus; hydrogen; operons; genomic libraries; oxidation; transcription factors; bacterial genomics; genome sequencing; dehydrogenases; gene regulation