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Title: Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

We report that serial femtosecond crystallography using ultrashort pulses from X-ray Free Electron Lasers (XFELs) offers the possibility to study light-triggered dynamics of biomolecules. Using microcrystals of the blue light photoreceptor, photoactive yellow protein, as a model system, we present high resolution, time-resolved difference electron density maps of excellent quality with strong features, which allow the determination of structures of reaction intermediates to 1.6 Å resolution. These results open the way to the study of reversible and non-reversible biological reactions on time scales as short as femtoseconds under conditions which maximize the extent of reaction initiation throughout the crystal.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [5] ;  [5] ;  [4] ;  [4] ;  [4] ;  [6] ;  [7] ;  [2] ;  [2] ;  [2] ;  [2] ;  [3] ;  [3] ;  [3] ;  [8] more »;  [7] ;  [7] ;  [1] ;  [9] ;  [6] ;  [10] ;  [10] ;  [1] ;  [2] ;  [1] ;  [11] ;  [12] ;  [3] ;  [2] ;  [9] ;  [1] « less
  1. Univ. of Wisconsin, Milwaukee, WI (United States). Physics Dept.
  2. Arizona State Univ., Tempe, AZ (United States). Dept. of Chemistry and Biochemistry
  3. Arizona State Univ., Tempe, AZ (United States). Dept. of Physics
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  5. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  6. Univ. of Hamburg (Germany). Centre for Ultrafast Imaging
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  8. State Univ. of New York at Buffalo (SUNY), Buffalo, NY (United States). Hauptman-Woodward Inst.
  9. Univ. of Hamburg (Germany). Centre for Ultrafast Imaging; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  10. Univ. of Chicago, IL (United States). Center for Advanced Radiation Sources
  11. Univ. of Chicago, IL (United States). Center for Advanced Radiation Sources; Univ. of Chicago, IL (United States). Dept. of Biochemistry and Molecular Biology and Inst. for Biophysical Dynamics
  12. Univ. of Chicago, IL (United States). Dept. of Biochemistry and Molecular Biology and Inst. for Biophysical Dynamics
Publication Date:
OSTI Identifier:
1165312
Grant/Contract Number:
NSF-1231306; R01GM095583; AC52-07NA27344; 012-ERD-031; R24GM111072
Type:
Accepted Manuscript
Journal Name:
Science
Additional Journal Information:
Journal Volume: 346; Journal Issue: 6214; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Research Org:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org:
USDOE; National Inst. of Health (NIH); National Science Foundation (NSF)
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION