skip to main content

DOE PAGESDOE PAGES

Title: Anti-contamination device for cryogenic soft X-ray diffraction microscopy

Cryogenic microscopy allows one to view frozen hydrated biological and soft matter specimens with good structural preservation and a high degree of stability against radiation damage. We describe a liquid nitrogen-cooled anti-contamination device for cryogenic X-ray diffraction microscopy. The anti-contaminator greatly reduces the buildup of ice layers on the specimen due to condensation of residual water vapor in the experimental vacuum chamber. We show by coherent X-ray diffraction measurements that this leads to fivefold reduction of background scattering, which is important for far-field X-ray diffraction microscopy of biological specimens.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [3] ;  [2] ;  [4]
  1. Stony Brook Univ., NY (United States) Dept. of Physics and Astronomy
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Northwestern Univ., Evanston, IL (United States) Dept. of Physics and Astronomy
  4. Stony Brook Univ., NY (United States) Dept. of Physics and Astronomy; Northwestern Univ., Evanston, IL (United States) Dept. of Physics and Astronomy; Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Proton Source (APS)
Publication Date:
OSTI Identifier:
1165057
Grant/Contract Number:
FG02-04ER46128
Type:
Accepted Manuscript
Journal Name:
Nuclear Instruments and Methods in Physics Research. Section A, Accelerators, Spectrometers, Detectors and Associated Equipment
Additional Journal Information:
Journal Volume: 638; Journal Issue: 1; Journal ID: ISSN 0168-9002
Publisher:
Elsevier
Research Org:
Stony Brook Univ., NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY X-RAY IMAGING; CRYOGENIC X-RAY MICROSCOPY; DIFFRACTION MICROSCOPY; ANTI-CONTAMINATION