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Title: Cryo diffraction microscopy: Ice conditions and finite supports

Using a signal-to-noise ratio estimation based on correlations between multiple simulated images, we compare the dose efficiency of two soft x-ray imaging systems: incoherent brightfield imaging using zone plate optics in a transmission x-ray microscope (TXM), and x-ray diffraction microscopy (XDM) where an image is reconstructed from the far-field coherent diffraction pattern. In XDM one must computationally phase weak diffraction signals; in TXM one suffers signal losses due to the finite numerical aperture and efficiency of the optics. In simulations with objects representing isolated cells such as yeast, we find that XDM has the potential for delivering equivalent resolution images using fewer photons. This can be an important advantage for studying radiation-sensitive biological and soft matter specimens.
Authors:
 [1] ;  [2] ;  [1] ;  [2] ;  [2] ;  [1] ;  [2] ;  [1] ;  [1] ;  [1]
  1. Stony Brook Univ., Stony Brook, NY (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
OSTI Identifier:
1164147
Grant/Contract Number:
FG02-04ER46128
Type:
Accepted Manuscript
Journal Name:
Journal of Physics. Conference Series (Online)
Additional Journal Information:
Journal Name: Journal of Physics. Conference Series (Online); Journal Volume: 186; Journal ID: ISSN 1742-6596
Publisher:
Institute of Physics (IOP)
Research Org:
Stony Brook Univ., Stony Brook, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE