skip to main content

DOE PAGESDOE PAGES

Title: Apparatus for X-ray diffraction microscopy and tomography of cryo specimens

An apparatus for diffraction microscopy of biological and materials science specimens is described. In this system, a coherent soft X-ray beam is selected with a pinhole, and the illuminated specimen is followed by an adjustable beamstop and CCD camera to record diffraction data from non-crystalline specimens. In addition, a Fresnel zone plate can be inserted to allow for direct imaging. The system makes use of a cryogenic specimen holder with cryotransfer capabilities to allow frozen hydrated specimens to be loaded. The specimen can be tilted over a range of ± 80 ° degrees for three-dimensional imaging; this is done by computer-controlled motors, enabling automated alignment of the specimen through a tilt series. The system is now in use for experiments in soft X-ray diffraction microscopy.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [1] ;  [5] ;  [1] ;  [3] ;  [1] ;  [1]
  1. Stony Brook Univ., Stony Brook, NY (United States)
  2. Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source; Brookhaven National Laboratory (BNL), Upton, NY (United States). Center for Functional Nanomaterials
  3. Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source
  4. Stony Brook Univ., Stony Brook, NY (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
  5. Stony Brook Univ., Stony Brook, NY (United States); TASC-INFM National Lab, Trieste (Italy)
Publication Date:
OSTI Identifier:
1164138
Grant/Contract Number:
FG02-04ER46128
Type:
Accepted Manuscript
Journal Name:
Nuclear Instruments and Methods in Physics Research. Section A, Accelerators, Spectrometers, Detectors and Associated Equipment
Additional Journal Information:
Journal Volume: 545; Journal Issue: 1-2; Journal ID: ISSN 0168-9002
Publisher:
Elsevier
Research Org:
Stony Brook Univ., Stony Brook, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION