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Title: High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures

Abstract

Subambient pressure ionization with nanoelectrospray (SPIN) has proven to be effective in producing ions with high efficiency and transmitting them to low pressures for high sensitivity mass spectrometry (MS) analysis. Here we present evidence that not only does the SPIN source improve MS sensitivity but also allows for gentler ionization conditions. The gentleness of a conventional heated capillary electrospray ionization (ESI) source and the SPIN source was compared by the liquid chromatography mass spectrometry (LC-MS) analysis of colominic acid. Colominic acid is a mixture of sialic acid polymers of different lengths containing labile glycosidic linkages between monomer units necessitating a gentle ion source. By coupling the SPIN source with high resolution mass spectrometry and using advanced data processing tools, we demonstrate much extended coverage of sialic acid polymer chains as compared to using the conventional ESI source. Additionally we show that SPIN-LC-MS is effective in elucidating polymer features with high efficiency and high sensitivity previously unattainable by the conventional ESI-LC-MS methods.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
+ Show Author Affiliations
  1. Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, United States
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
OSTI Identifier:
1159260
Alternate Identifier(s):
OSTI ID: 1167611
Report Number(s):
PNNL-SA-102134
Journal ID: ISSN 0003-2700
Grant/Contract Number:  
Pan-omics; AC05-76RLO01830; 1R33CA155252; GM103493-1
Resource Type:
Published Article
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Name: Analytical Chemistry Journal Volume: 86 Journal Issue: 19; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Environmental Molecular Sciences Laboratory

Citation Formats

Cox, Jonathan T., Kronewitter, Scott R., Shukla, Anil K., Moore, Ronald J., Smith, Richard D., and Tang, Keqi. High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures. United States: N. p., 2014. Web. doi:10.1021/ac502767y.
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Cox, Jonathan T., Kronewitter, Scott R., Shukla, Anil K., Moore, Ronald J., Smith, Richard D., & Tang, Keqi. High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures. United States. https://doi.org/10.1021/ac502767y
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Cox, Jonathan T., Kronewitter, Scott R., Shukla, Anil K., Moore, Ronald J., Smith, Richard D., and Tang, Keqi. Fri . "High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures". United States. https://doi.org/10.1021/ac502767y.
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@article{osti_1159260,
title = {High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures},
author = {Cox, Jonathan T. and Kronewitter, Scott R. and Shukla, Anil K. and Moore, Ronald J. and Smith, Richard D. and Tang, Keqi},
abstractNote = {Subambient pressure ionization with nanoelectrospray (SPIN) has proven to be effective in producing ions with high efficiency and transmitting them to low pressures for high sensitivity mass spectrometry (MS) analysis. Here we present evidence that not only does the SPIN source improve MS sensitivity but also allows for gentler ionization conditions. The gentleness of a conventional heated capillary electrospray ionization (ESI) source and the SPIN source was compared by the liquid chromatography mass spectrometry (LC-MS) analysis of colominic acid. Colominic acid is a mixture of sialic acid polymers of different lengths containing labile glycosidic linkages between monomer units necessitating a gentle ion source. By coupling the SPIN source with high resolution mass spectrometry and using advanced data processing tools, we demonstrate much extended coverage of sialic acid polymer chains as compared to using the conventional ESI source. Additionally we show that SPIN-LC-MS is effective in elucidating polymer features with high efficiency and high sensitivity previously unattainable by the conventional ESI-LC-MS methods.},
doi = {10.1021/ac502767y},
journal = {Analytical Chemistry},
number = 19,
volume = 86,
place = {United States},
year = {Fri Sep 26 00:00:00 EDT 2014},
month = {Fri Sep 26 00:00:00 EDT 2014}
}
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https://doi.org/10.1021/ac502767y
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