The Semiquinone at the Q i Site of the bc 1 Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via 13 C Methionine and Construction of a Methionine Auxotroph

Hong, Sangjin; de Almeida, Wagner B.; Taguchi, Alexander T.; Samoilova, Rimma I.; Gennis, Robert B.; O’Malley, Patrick J.; Dikanov, Sergei A.; Crofts, Antony R.

doi:10.1021/bi500654y

Title: The Semiquinone at the Q _i Site of the bc ₁ Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via ¹³ C Methionine and Construction of a Methionine Auxotroph

Authors:

Hong, Sangjin ^[1]; de Almeida, Wagner B. ^[2]; Taguchi, Alexander T. ^[3]; Samoilova, Rimma I. ^[4]; Gennis, Robert B. ^[1]; O’Malley, Patrick J. ^[2]; Dikanov, Sergei A. ^[5]; Crofts, Antony R. ^[1]

Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States
School of Chemistry, The University of Manchester, Manchester M13 9PL, U.K.
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States
V. V. Voevodsky Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Novosibirsk 630090, Russian Federation
Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States

Publication Date:: Wed Sep 17 00:00:00 EDT 2014

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1158872

Grant/Contract Number:: FG02-08ER15960; FG02-87ER13716

Resource Type:: Published Article

Journal Name:: Biochemistry

Additional Journal Information:: Journal Name: Biochemistry Journal Volume: 53 Journal Issue: 38; Journal ID: ISSN 0006-2960

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Citation Formats


                    Hong, Sangjin, de Almeida, Wagner B., Taguchi, Alexander T., Samoilova, Rimma I., Gennis, Robert B., O’Malley, Patrick J., Dikanov, Sergei A., and Crofts, Antony R. The Semiquinone at the Q i Site of the bc 1 Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via 13 C Methionine and Construction of a Methionine Auxotroph.  United States: N. p., 2014. 
Web.  doi:10.1021/bi500654y.

Copy to clipboard


                    Hong, Sangjin, de Almeida, Wagner B., Taguchi, Alexander T., Samoilova, Rimma I., Gennis, Robert B., O’Malley, Patrick J., Dikanov, Sergei A., & Crofts, Antony R. The Semiquinone at the Q i Site of the bc 1 Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via 13 C Methionine and Construction of a Methionine Auxotroph.  United States.  https://doi.org/10.1021/bi500654y

Copy to clipboard


                    Hong, Sangjin, de Almeida, Wagner B., Taguchi, Alexander T., Samoilova, Rimma I., Gennis, Robert B., O’Malley, Patrick J., Dikanov, Sergei A., and Crofts, Antony R. Wed .  
"The Semiquinone at the Q i Site of the bc 1 Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via 13 C Methionine and Construction of a Methionine Auxotroph".  United States.  https://doi.org/10.1021/bi500654y.

Copy to clipboard


                    
@article{osti_1158872,

  title        = {The Semiquinone at the Q i Site of the bc 1 Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via 13 C Methionine and Construction of a Methionine Auxotroph},

  author       = {Hong, Sangjin and de Almeida, Wagner B. and Taguchi, Alexander T. and Samoilova, Rimma I. and Gennis, Robert B. and O’Malley, Patrick J. and Dikanov, Sergei A. and Crofts, Antony R.},

  abstractNote = {},

  doi          = {10.1021/bi500654y},

  journal      = {Biochemistry},

  number       = 38,

  volume       = 53,

  place        = {United States},

  year         = {Wed Sep 17 00:00:00 EDT 2014},

  month        = {Wed Sep 17 00:00:00 EDT 2014}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Publisher's Version of Record
https://doi.org/10.1021/bi500654y

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 13 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Hydrogen Bonds Involved in Binding the Qi-site Semiquinone in the bc1 Complex, Identified through Deuterium Exchange Using Pulsed EPR
journal, April 2004

Dikanov, Sergei A.; Samoilova, Rimma I.; Kolling, Derrick R. J.
Journal of Biological Chemistry, Vol. 279, Issue 16
DOI: 10.1074/jbc.M313417200

Phenotypic and genetic characterization of cytochrome c2-deficient mutants of Rhodobacter sphaeroides
journal, March 1988

Donohue, Timothy J.; McEwan, Alastair G.; Van Doren, Steven
Biochemistry, Vol. 27, Issue 6
DOI: 10.1021/bi00406a018

Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
journal, December 2001

Lange, C.
The EMBO Journal, Vol. 20, Issue 23
DOI: 10.1093/emboj/20.23.6591

Inhibitor-complexed Structures of the Cytochrome bc ₁ from the Photosynthetic Bacterium Rhodobacter sphaeroides
journal, November 2007

Esser, Lothar; Elberry, Maria; Zhou, Fei
Journal of Biological Chemistry, Vol. 283, Issue 5
DOI: 10.1074/jbc.M708608200

Function of the iron-sulfur protein of the cytochrome b-c1 segment in electron-transfer and energy-conserving reactions of the mitochondrial respiratory chain
journal, December 1981

Trumpower, Bernard L.
Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, Vol. 639, Issue 2
DOI: 10.1016/0304-4173(81)90008-2

Resolving the EPR Spectra in the Cytochrome bc 1 Complex from Saccharomyces cerevisiae
journal, November 2009

MacMillan, Fraser; Lange, Christian; Bawn, Matt
Applied Magnetic Resonance, Vol. 37, Issue 1-4
DOI: 10.1007/s00723-009-0077-4

Structural Basis for the Quinone Reduction in the bc ₁ Complex: A Comparative Analysis of Crystal Structures of Mitochondrial Cytochrome bc ₁ with Bound Substrate and Inhibitors at the Q _i Site ^† ^, ^‡
journal, August 2003

Gao, Xiugong; Wen, Xiaoling; Esser, Lothar
Biochemistry, Vol. 42, Issue 30
DOI: 10.1021/bi0341814

Hydrogen Bonding and Spin Density Distribution in the Q _B Semiquinone of Bacterial Reaction Centers and Comparison with the Q _A Site
journal, April 2011

Martin, Erik; Samoilova, Rimma I.; Narasimhulu, Kupala V.
Journal of the American Chemical Society, Vol. 133, Issue 14
DOI: 10.1021/ja2001538

Interactions of Intermediate Semiquinone with Surrounding Protein Residues at the Q _H Site of Wild-Type and D75H Mutant Cytochrome bo ₃ from Escherichia coli
journal, April 2012

Lin, Myat T.; Baldansuren, Amgalanbaatar; Hart, Richard
Biochemistry, Vol. 51, Issue 18
DOI: 10.1021/bi300151q

Reductive titration of CoQ-depleted Complex III from Baker's yeast: Evidence for an exchange-coupled complex between QH· and low-spin ferricytochrome b
journal, October 1983

de la Rosa, F. F.; Palmer, G.
FEBS Letters, Vol. 163, Issue 1
DOI: 10.1016/0014-5793(83)81181-8

Hydrogen Bonding between the Q _B Site Ubisemiquinone and Ser-L223 in the Bacterial Reaction Center: A Combined Spectroscopic and Computational Perspective
journal, October 2012

Martin, Erik; Baldansuren, Amgalanbaatar; Lin, Tzu-Jen
Biochemistry, Vol. 51, Issue 45
DOI: 10.1021/bi300834w

Cloning and DNA sequencing of the fbc operon encoding the cytochrome bc1 complex from Rhodobacter sphaeroides. Characterization of fbc deletion mutants and complementation by a site-specific mutational variant
journal, December 1990

Yun, Chang-Hyon; Beci, Rose; Crofts, Antony R.
European Journal of Biochemistry, Vol. 194, Issue 2
DOI: 10.1111/j.1432-1033.1990.tb15633.x

Electron nuclear double resonance (ENDOR) of the Qc.cntdot.- ubisemiquinone radical in the mitochondrial electron transport chain
journal, July 1990

Salerno, John C.; Osgood, Marcy; Liu, Yajun
Biochemistry, Vol. 29, Issue 30
DOI: 10.1021/bi00482a006

A compilation of mutations located in the cytochrome b subunit of the bacterial and mitochondrial bc1 complex
journal, July 1996

Brasseur, Gael; Saribaş, A. Sami; Daldal, Fevzi
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1275, Issue 1-2
DOI: 10.1016/0005-2728(96)00051-5

Hydrogen Bonds between Nitrogen Donors and the Semiquinone in the Qi-site of the bc1 Complex
journal, August 2007

Dikanov, Sergei A.; Holland, J. Todd; Endeward, Burkhard
Journal of Biological Chemistry, Vol. 282, Issue 35
DOI: 10.1074/jbc.M702333200

Secondary electron transfer in chromatophores of Rhodopseudomonas capsulata A1a pho
journal, May 1979

Bowyer, J. R.; Tierney, G. V.; Crofts, A. R.
FEBS Letters, Vol. 101, Issue 1, p. 201-206
DOI: 10.1016/0014-5793(79)81326-5

Conformational Differences between the Methoxy Groups of Q _A and Q _B Site Ubisemiquinones in Bacterial Reaction Centers: A Key Role for Methoxy Group Orientation in Modulating Ubiquinone Redox Potential
journal, June 2013

Taguchi, Alexander T.; O’Malley, Patrick J.; Wraight, Colin A.
Biochemistry, Vol. 52, Issue 27
DOI: 10.1021/bi400489b

The Q-cycle reviewed: How well does a monomeric mechanism of the bc1 complex account for the function of a dimeric complex?
journal, July 2008

Crofts, Antony R.; Holland, J. Todd; Victoria, Doreen
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1777, Issue 7-8
DOI: 10.1016/j.bbabio.2008.04.037

The mechanism of ubihydroquinone oxidation at the Qo-site of the cytochrome bc1 complex
journal, November 2013

Crofts, Antony R.; Hong, Sangjin; Wilson, Charles
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 11-12
DOI: 10.1016/j.bbabio.2013.01.009

The Q-cycle – A Personal Perspective
journal, January 2004

Crofts, Antony R.
Photosynthesis Research, Vol. 80, Issue 1-3
DOI: 10.1023/B:PRES.0000030444.52579.10

The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
journal, January 1994

Lenz, O.; Schwartz, E.; Dernedde, J.
Journal of Bacteriology, Vol. 176, Issue 14
DOI: 10.1128/jb.176.14.4385-4393.1994

Thermodynamic properties of the semiquinone and its binding site in the ubiquinol-cytochrome c (c2) oxidoreductase of respiratory and photosynthetic systems.
journal, February 1984

Robertson, D. E.; Prince, R. C.; Bowyer, J. R.
Journal of Biological Chemistry, Vol. 259, Issue 3
DOI: 10.1016/S0021-9258(17)43472-7

Characterization of mutations in the cytochrome b subunit of the bc1 complex of Rhodobacter sphaeroides that affect the quinone reductase site (Qc)
journal, April 1993

Hacker, Beth; Barquera, Blanca; Crofts, Antony R.
Biochemistry, Vol. 32, Issue 16
DOI: 10.1021/bi00067a033

X-Ray Structure of Rhodobacter Capsulatus Cytochrome bc ₁ : Comparison with its Mitochondrial and Chloroplast Counterparts
journal, January 2004

Berry, Edward A.; Huang, Li-Shar; Saechao, Lai K.
Photosynthesis Research, Vol. 81, Issue 3
DOI: 10.1023/B:PRES.0000036888.18223.0e

[21] Redox flow as an instrument of gene regulation
book, January 2002

Eraso, Jesus M.; Kaplan, Samuel
Protein Sensors and Reactive Oxygen Species - Part B: Thiol Enzymes and Proteins
DOI: 10.1016/S0076-6879(02)48640-5

Evolution of Photosynthesis: Time-Independent Structure of the Cytochrome b ₆ f Complex ^†
journal, May 2004

Cramer, William A.; Zhang, Huamin; Yan, Jiusheng
Biochemistry, Vol. 43, Issue 20
DOI: 10.1021/bi049444o

Chromatophore heterogeneity explains phenomena seen in Rhodobacter sphaeroides previously attributed to supercomplexes
journal, January 1998

Crofts, Antony; Guergova-Kuras, Mariana; Hong, Sangjin
Photosynthesis Research, Vol. 55, Issue 2/3, p. 357-362
DOI: 10.1023/A:1005940120396

Hyperfine sublevel correlation (hyscore) spectroscopy: a 2D ESR investigation of the squaric acid radical
journal, December 1986

Höfer, P.; Grupp, A.; Nebenführ, H.
Chemical Physics Letters, Vol. 132, Issue 3
DOI: 10.1016/0009-2614(86)80124-5

Crystal Structure of the Cytochrome bc ₁ Complex from Bovine Heart Mitochondria
journal, July 1997

Xia, Di; Yu, Chang-An; Kim, Hoeon
Science, Vol. 277, Issue 5322
DOI: 10.1126/science.277.5322.60

Exploration of Ligands to the Qi Site Semiquinone in the bc 1 Complex Using High-resolution EPR
journal, October 2003

Kolling, Derrick R. J.; Samoilova, Rimma I.; Holland, J. Todd
Journal of Biological Chemistry, Vol. 278, Issue 41
DOI: 10.1074/jbc.M305913200

Complete Structure of the 11-Subunit Bovine Mitochondrial Cytochrome bc1 Complex
journal, July 1998

Iwata, S.
Science, Vol. 281, Issue 5373
DOI: 10.1126/science.281.5373.64

Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation
journal, August 1966

Mitchell, Peter
Biological Reviews, Vol. 41, Issue 3
DOI: 10.1111/j.1469-185X.1966.tb01501.x

Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1
journal, October 1999

Mackenzie, Chris; Simmons, Adrian E.; Kaplan, Samuel
Genetics, Vol. 153, Issue 2
DOI: 10.1093/genetics/153.2.525

Requirement of histidine 217 for ubiquinone reductase activity (Qi site) in the cytochrome bc1 complex
journal, January 1994

Gray, Kevin A.; Dutton, P. Leslie; Daldal, Fevzi
Biochemistry, Vol. 33, Issue 3
DOI: 10.1021/bi00169a014

Electron transfer by domain movement in cytochrome bc1
journal, April 1998

Zhang, Zhaolei; Huang, Lishar; Shulmeister, Vladimir M.
Nature, Vol. 392, Issue 6677
DOI: 10.1038/33612

Function and Properties of a Semiquinone Anion Located in the QH2: Cytochrome c Oxidoreductase Segment of the Respiratory Chain
book, January 1982

De Vries, Simon; Berden, J. A.; Slater, E. C.
Function of Quinones in Energy Conserving Systems
DOI: 10.1016/B978-0-12-701280-3.50022-0

Possible molecular mechanisms of the protonmotive function of cytochrome systems
journal, October 1976

Mitchell, Peter
Journal of Theoretical Biology, Vol. 62, Issue 2
DOI: 10.1016/0022-5193(76)90124-7

Cross-Peak Lineshape of Two-Dimensional ESEEM Spectra in Disordered S = 12, I = 12 Spin Systems
journal, September 1995

Dikanov, S. A.; Bowman, M. K.
Journal of Magnetic Resonance, Series A, Vol. 116, Issue 1
DOI: 10.1006/jmra.1995.1199

Binding of the Respiratory Chain Inhibitor Antimycin to the Mitochondrial bc1 Complex: A New Crystal Structure Reveals an Altered Intramolecular Hydrogen-bonding Pattern
journal, August 2005

Huang, Li-shar; Cobessi, David; Tung, Eric Y.
Journal of Molecular Biology, Vol. 351, Issue 3
DOI: 10.1016/j.jmb.2005.05.053

ENDOR and EPR studies of highly isotopically 13C-enriched ubiquinone radicals. Part 2
journal, January 1995

Samoilova, Rimma I.; Gritsan, Nina P.; Hoff, Arnold J.
Journal of the Chemical Society, Perkin Transactions 2, Issue 11
DOI: 10.1039/p29950002063

Rieske Protein from Thermus t hermophilus : ¹⁵ N NMR Titration Study Demonstrates the Role of Iron-Ligated Histidines in the pH Dependence of the Reduction Potential
journal, August 2006

Lin, I-Jin; Chen, Ying; Fee, James A.
Journal of the American Chemical Society, Vol. 128, Issue 33
DOI: 10.1021/ja0627388

The Cytochrome bc ₁ Complex: Function in the Context of Structure
journal, March 2004

Crofts, Antony R.
Annual Review of Physiology, Vol. 66, Issue 1
DOI: 10.1146/annurev.physiol.66.032102.150251

The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides A modified Q-cycle mechanism
journal, May 1983

Crofts, A. R.; Meinhardt, S. W.; Jones, K. R.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 723, Issue 2
DOI: 10.1016/0005-2728(83)90120-2

An ONIOM study of the QA site semiquinone in the Rhodobacter sphaeroides photosynthetic reaction centre
journal, December 2008

Lin, Tzu-Jen; O’Malley, Patrick J.
Journal of Molecular Structure: THEOCHEM, Vol. 870, Issue 1-3
DOI: 10.1016/j.theochem.2008.08.034

Exploring by Pulsed EPR the Electronic Structure of Ubisemiquinone Bound at the QH Site of Cytochrome bo3 from Escherichia coli with in Vivo 13C-Labeled Methyl and Methoxy Substituents*
journal, March 2011

Lin, Myat T.; Shubin, Alexander A.; Samoilova, Rimma I.
Journal of Biological Chemistry, Vol. 286, Issue 12
DOI: 10.1074/jbc.M110.206821

Similar Records in DOE PAGES and OSTI.GOV collections:

C magic angle spinning NMR characterization of the functionally asymmetric Q{sub A} binding in Rhodobacter sphaeroides R26 photosynthetic reaction centers using site-specific {sup 13}C-labeled ubiqinone-10

Journal Article Liemt, W B.S. van ; Boender, G J ; Lugtenburg, J - Biochemistry (Eaton)

Photosynthetic reaction centers (RCs) of Rhodobacter sphaeroides R26 were reconstituted at the Q{sub A} site with ubiquinone-10, selectively {sup 13}C-enriched on positions 1, 2, 3, 4, and 3-Me (IUPAC numbering). RCs dispersed in LDAO detergent were studied with {sup 13}C CP/MAS NMR spectroscopy at temperatures between 180 and 240 K, while RCs precipitated by removal of the detergent were investigated at ambient temperature and at temperatures down to 180 K. Electrostatic charge differences in Q{sub A} induced by polarization from the protein are less than 0.02 electronic equivalent for any of the labeled positions. This includes the 4-carbonyl, which ismore »« less
https://doi.org/10.1021/bi00032a017
Binding sites of quinones in photosynthetic bacterial reaction centers investigated by light-induced FTIR difference spectroscopy: Symmetry of the carbonyl interactions and close equivalence of the Q{sub B} vibrations in Rhodobacter sphaeroides and Rhodopseudomonas viridis probed by isotope labeling

Journal Article Breton, J ; Berger, G ; Nabedryk, E - Biochemistry (Eaton)

The photoreduction of the secondary quinone acceptor Q{sub B} in reaction centers (RCs) of the photosynthetic bacteria Rhodobacter sphaeroides and Rhodopseudomonas viridis has been investigated by light-induced FTIR difference spectroscopy of RCs reconstituted with several isotopically labeled ubiquinones. The labels used were {sup 18}O on both carbonyls and {sup 13}C either uniformly or selectively at the 1- or the 4-position, i.e., on either one of the two carbonyls. The Q{sub B}{sup {minus}}/Q{sub B} spectra of RCs reconstituted with the isotopically labeled and unlabeled quinones as well as the double differences calculated form these spectra exhibit distinct isotopic shifts for amore »« less
https://doi.org/10.1021/bi00036a037
Unpaired Electron Spin Density Distribution across Reduced [2Fe-2S] Cluster Ligands by ¹³ C _β -Cysteine Labeling

Journal Article Taguchi, Alexander T. ; Miyajima-Nakano, Yoshiharu ; Fukazawa, Risako ; ... - Inorganic Chemistry

Not provided.
https://doi.org/10.1021/acs.inorgchem.7b02676
Nuclear Hyperfine and Quadrupole Tensor Characterization of the Nitrogen Hydrogen Bond Donors to the Semiquinone of the Q _B Site in Bacterial Reaction Centers: A Combined X- and S-Band ^14,15 N ESEEM and DFT Study

Journal Article Taguchi, Alexander T. ; O’Malley, Patrick J. ; Wraight, Colin A. ; ... - Journal of Physical Chemistry. B
Cited by 17
https://doi.org/10.1021/jp411023k
Electron spin echo envelope modulation spectroscopy supports the suggested coordination of two histidine ligands to the Rieske Fe-S centers of the cytochrome b sub 6 f complex of spinach and the cytochrome bc sub 1 complexes of Rhodospirillum rubrum, Rhodobacter sphaeroides R-26, and bovine heart mitochondria

Journal Article Britt, R D ; Sauer, K ; Klein, M P ; ... - Biochemistry; (United States)

Electron spin echo envelope modulation (ESEEM) experiments performed on the Rieske Fe-S clusters of the cytochrome b{sub 6}f complex of spinach chloroplasts and of the cytochrome bc{sub 1} complexes of Rhodospirillum rubrum, Rhodobacter sphaeroides R-26, and bovine heart mitochondria show modulation components resulting from two distinct classes of {sup 14}N ligands. At the g = 1.92 region of the Rieske EPR spectrum of the cytochrome b{sub 6}f complex, the measured hyperfine couplings for the two classes of coupled nitrogens are A{sub 1} = 4.6 MHz and A{sub 2} = 3.8 MHz. Similar couplings are observed for the Rieske centers inmore »« less
https://doi.org/10.1021/bi00221a023

Similar Records

Title: The Semiquinone at the Q i Site of the bc 1 Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via 13 C Methionine and Construction of a Methionine Auxotroph

Citation Formats

Hydrogen Bonds Involved in Binding the Qi-site Semiquinone in the bc1 Complex, Identified through Deuterium Exchange Using Pulsed EPR journal, April 2004

Phenotypic and genetic characterization of cytochrome c2-deficient mutants of Rhodobacter sphaeroides journal, March 1988

Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure journal, December 2001

Inhibitor-complexed Structures of the Cytochrome bc 1 from the Photosynthetic Bacterium Rhodobacter sphaeroides journal, November 2007

Function of the iron-sulfur protein of the cytochrome b-c1 segment in electron-transfer and energy-conserving reactions of the mitochondrial respiratory chain journal, December 1981

Resolving the EPR Spectra in the Cytochrome bc 1 Complex from Saccharomyces cerevisiae journal, November 2009

Structural Basis for the Quinone Reduction in the bc 1 Complex: A Comparative Analysis of Crystal Structures of Mitochondrial Cytochrome bc 1 with Bound Substrate and Inhibitors at the Q i Site † , ‡ journal, August 2003

Hydrogen Bonding and Spin Density Distribution in the Q B Semiquinone of Bacterial Reaction Centers and Comparison with the Q A Site journal, April 2011

Interactions of Intermediate Semiquinone with Surrounding Protein Residues at the Q H Site of Wild-Type and D75H Mutant Cytochrome bo 3 from Escherichia coli journal, April 2012

Reductive titration of CoQ-depleted Complex III from Baker's yeast: Evidence for an exchange-coupled complex between QH· and low-spin ferricytochrome b journal, October 1983

Hydrogen Bonding between the Q B Site Ubisemiquinone and Ser-L223 in the Bacterial Reaction Center: A Combined Spectroscopic and Computational Perspective journal, October 2012

Cloning and DNA sequencing of the fbc operon encoding the cytochrome bc1 complex from Rhodobacter sphaeroides. Characterization of fbc deletion mutants and complementation by a site-specific mutational variant journal, December 1990

Electron nuclear double resonance (ENDOR) of the Qc.cntdot.- ubisemiquinone radical in the mitochondrial electron transport chain journal, July 1990

A compilation of mutations located in the cytochrome b subunit of the bacterial and mitochondrial bc1 complex journal, July 1996

Hydrogen Bonds between Nitrogen Donors and the Semiquinone in the Qi-site of the bc1 Complex journal, August 2007

Secondary electron transfer in chromatophores of Rhodopseudomonas capsulata A1a pho journal, May 1979

Conformational Differences between the Methoxy Groups of Q A and Q B Site Ubisemiquinones in Bacterial Reaction Centers: A Key Role for Methoxy Group Orientation in Modulating Ubiquinone Redox Potential journal, June 2013

The Q-cycle reviewed: How well does a monomeric mechanism of the bc1 complex account for the function of a dimeric complex? journal, July 2008

The mechanism of ubihydroquinone oxidation at the Qo-site of the cytochrome bc1 complex journal, November 2013

The Q-cycle – A Personal Perspective journal, January 2004

The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation. journal, January 1994

Thermodynamic properties of the semiquinone and its binding site in the ubiquinol-cytochrome c (c2) oxidoreductase of respiratory and photosynthetic systems. journal, February 1984

Characterization of mutations in the cytochrome b subunit of the bc1 complex of Rhodobacter sphaeroides that affect the quinone reductase site (Qc) journal, April 1993

X-Ray Structure of Rhodobacter Capsulatus Cytochrome bc 1 : Comparison with its Mitochondrial and Chloroplast Counterparts journal, January 2004

[21] Redox flow as an instrument of gene regulation book, January 2002

Evolution of Photosynthesis: Time-Independent Structure of the Cytochrome b 6 f Complex † journal, May 2004

Chromatophore heterogeneity explains phenomena seen in Rhodobacter sphaeroides previously attributed to supercomplexes journal, January 1998

Hyperfine sublevel correlation (hyscore) spectroscopy: a 2D ESR investigation of the squaric acid radical journal, December 1986

Crystal Structure of the Cytochrome bc 1 Complex from Bovine Heart Mitochondria journal, July 1997

Exploration of Ligands to the Qi Site Semiquinone in the bc 1 Complex Using High-resolution EPR journal, October 2003

Complete Structure of the 11-Subunit Bovine Mitochondrial Cytochrome bc1 Complex journal, July 1998

Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation journal, August 1966

Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1 journal, October 1999

Requirement of histidine 217 for ubiquinone reductase activity (Qi site) in the cytochrome bc1 complex journal, January 1994

Electron transfer by domain movement in cytochrome bc1 journal, April 1998

Function and Properties of a Semiquinone Anion Located in the QH2: Cytochrome c Oxidoreductase Segment of the Respiratory Chain book, January 1982

Possible molecular mechanisms of the protonmotive function of cytochrome systems journal, October 1976

Cross-Peak Lineshape of Two-Dimensional ESEEM Spectra in Disordered S = 12, I = 12 Spin Systems journal, September 1995

Binding of the Respiratory Chain Inhibitor Antimycin to the Mitochondrial bc1 Complex: A New Crystal Structure Reveals an Altered Intramolecular Hydrogen-bonding Pattern journal, August 2005

ENDOR and EPR studies of highly isotopically 13C-enriched ubiquinone radicals. Part 2 journal, January 1995

Rieske Protein from Thermus t hermophilus : 15 N NMR Titration Study Demonstrates the Role of Iron-Ligated Histidines in the pH Dependence of the Reduction Potential journal, August 2006

The Cytochrome bc 1 Complex: Function in the Context of Structure journal, March 2004

The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides A modified Q-cycle mechanism journal, May 1983

An ONIOM study of the QA site semiquinone in the Rhodobacter sphaeroides photosynthetic reaction centre journal, December 2008

Exploring by Pulsed EPR the Electronic Structure of Ubisemiquinone Bound at the QH Site of Cytochrome bo3 from Escherichia coli with in Vivo 13C-Labeled Methyl and Methoxy Substituents* journal, March 2011

Title: The Semiquinone at the Q _i Site of the bc ₁ Complex Explored Using HYSCORE Spectroscopy and Specific Isotopic Labeling of Ubiquinone in Rhodobacter sphaeroides via ¹³ C Methionine and Construction of a Methionine Auxotroph

Hydrogen Bonds Involved in Binding the Qi-site Semiquinone in the bc1 Complex, Identified through Deuterium Exchange Using Pulsed EPR
journal, April 2004

Phenotypic and genetic characterization of cytochrome c2-deficient mutants of Rhodobacter sphaeroides
journal, March 1988

Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure
journal, December 2001

Inhibitor-complexed Structures of the Cytochrome bc ₁ from the Photosynthetic Bacterium Rhodobacter sphaeroides
journal, November 2007

Function of the iron-sulfur protein of the cytochrome b-c1 segment in electron-transfer and energy-conserving reactions of the mitochondrial respiratory chain
journal, December 1981

Resolving the EPR Spectra in the Cytochrome bc 1 Complex from Saccharomyces cerevisiae
journal, November 2009

Structural Basis for the Quinone Reduction in the bc ₁ Complex: A Comparative Analysis of Crystal Structures of Mitochondrial Cytochrome bc ₁ with Bound Substrate and Inhibitors at the Q _i Site ^† ^, ^‡
journal, August 2003

Hydrogen Bonding and Spin Density Distribution in the Q _B Semiquinone of Bacterial Reaction Centers and Comparison with the Q _A Site
journal, April 2011

Interactions of Intermediate Semiquinone with Surrounding Protein Residues at the Q _H Site of Wild-Type and D75H Mutant Cytochrome bo ₃ from Escherichia coli
journal, April 2012

Reductive titration of CoQ-depleted Complex III from Baker's yeast: Evidence for an exchange-coupled complex between QH· and low-spin ferricytochrome b
journal, October 1983

Hydrogen Bonding between the Q _B Site Ubisemiquinone and Ser-L223 in the Bacterial Reaction Center: A Combined Spectroscopic and Computational Perspective
journal, October 2012

Cloning and DNA sequencing of the fbc operon encoding the cytochrome bc1 complex from Rhodobacter sphaeroides. Characterization of fbc deletion mutants and complementation by a site-specific mutational variant
journal, December 1990

Electron nuclear double resonance (ENDOR) of the Qc.cntdot.- ubisemiquinone radical in the mitochondrial electron transport chain
journal, July 1990

A compilation of mutations located in the cytochrome b subunit of the bacterial and mitochondrial bc1 complex
journal, July 1996

Hydrogen Bonds between Nitrogen Donors and the Semiquinone in the Qi-site of the bc1 Complex
journal, August 2007

Secondary electron transfer in chromatophores of Rhodopseudomonas capsulata A1a pho
journal, May 1979

Conformational Differences between the Methoxy Groups of Q _A and Q _B Site Ubisemiquinones in Bacterial Reaction Centers: A Key Role for Methoxy Group Orientation in Modulating Ubiquinone Redox Potential
journal, June 2013

The Q-cycle reviewed: How well does a monomeric mechanism of the bc1 complex account for the function of a dimeric complex?
journal, July 2008

The mechanism of ubihydroquinone oxidation at the Qo-site of the cytochrome bc1 complex
journal, November 2013

The Q-cycle – A Personal Perspective
journal, January 2004

The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
journal, January 1994

Thermodynamic properties of the semiquinone and its binding site in the ubiquinol-cytochrome c (c2) oxidoreductase of respiratory and photosynthetic systems.
journal, February 1984

Characterization of mutations in the cytochrome b subunit of the bc1 complex of Rhodobacter sphaeroides that affect the quinone reductase site (Qc)
journal, April 1993

X-Ray Structure of Rhodobacter Capsulatus Cytochrome bc ₁ : Comparison with its Mitochondrial and Chloroplast Counterparts
journal, January 2004

[21] Redox flow as an instrument of gene regulation
book, January 2002

Evolution of Photosynthesis: Time-Independent Structure of the Cytochrome b ₆ f Complex ^†
journal, May 2004

Chromatophore heterogeneity explains phenomena seen in Rhodobacter sphaeroides previously attributed to supercomplexes
journal, January 1998

Hyperfine sublevel correlation (hyscore) spectroscopy: a 2D ESR investigation of the squaric acid radical
journal, December 1986

Crystal Structure of the Cytochrome bc ₁ Complex from Bovine Heart Mitochondria
journal, July 1997

Exploration of Ligands to the Qi Site Semiquinone in the bc 1 Complex Using High-resolution EPR
journal, October 2003

Complete Structure of the 11-Subunit Bovine Mitochondrial Cytochrome bc1 Complex
journal, July 1998

Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation
journal, August 1966

Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1
journal, October 1999

Requirement of histidine 217 for ubiquinone reductase activity (Qi site) in the cytochrome bc1 complex
journal, January 1994

Electron transfer by domain movement in cytochrome bc1
journal, April 1998

Function and Properties of a Semiquinone Anion Located in the QH2: Cytochrome c Oxidoreductase Segment of the Respiratory Chain
book, January 1982

Possible molecular mechanisms of the protonmotive function of cytochrome systems
journal, October 1976

Cross-Peak Lineshape of Two-Dimensional ESEEM Spectra in Disordered S = 12, I = 12 Spin Systems
journal, September 1995

Binding of the Respiratory Chain Inhibitor Antimycin to the Mitochondrial bc1 Complex: A New Crystal Structure Reveals an Altered Intramolecular Hydrogen-bonding Pattern
journal, August 2005

ENDOR and EPR studies of highly isotopically 13C-enriched ubiquinone radicals. Part 2
journal, January 1995

Rieske Protein from Thermus t hermophilus : ¹⁵ N NMR Titration Study Demonstrates the Role of Iron-Ligated Histidines in the pH Dependence of the Reduction Potential
journal, August 2006

The Cytochrome bc ₁ Complex: Function in the Context of Structure
journal, March 2004

The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides A modified Q-cycle mechanism
journal, May 1983

An ONIOM study of the QA site semiquinone in the Rhodobacter sphaeroides photosynthetic reaction centre
journal, December 2008

Exploring by Pulsed EPR the Electronic Structure of Ubisemiquinone Bound at the QH Site of Cytochrome bo3 from Escherichia coli with in Vivo 13C-Labeled Methyl and Methoxy Substituents*
journal, March 2011