The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition

Turco, Michelle Marian; Sousa, Marcelo Carlos

doi:10.1021/bi500593e

Title: The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition

Authors:

Turco, Michelle Marian ^[1]; Sousa, Marcelo Carlos ^[1]

Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309-0596, United States

Publication Date:: Mon Jul 28 00:00:00 EDT 2014

Sponsoring Org.:: USDOE

OSTI Identifier:: 1158714

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Biochemistry

Additional Journal Information:: Journal Name: Biochemistry Journal Volume: 53 Journal Issue: 31; Journal ID: ISSN 0006-2960

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Citation Formats


                    Turco, Michelle Marian, and Sousa, Marcelo Carlos. The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition.  United States: N. p., 2014. 
Web.  doi:10.1021/bi500593e.

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                    Turco, Michelle Marian, & Sousa, Marcelo Carlos. The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition.  United States.  https://doi.org/10.1021/bi500593e

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                    Turco, Michelle Marian, and Sousa, Marcelo Carlos. Mon .  
"The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition".  United States.  https://doi.org/10.1021/bi500593e.

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@article{osti_1158714,

  title        = {The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition},

  author       = {Turco, Michelle Marian and Sousa, Marcelo Carlos},

  abstractNote = {},

  doi          = {10.1021/bi500593e},

  journal      = {Biochemistry},

  number       = 31,

  volume       = 53,

  place        = {United States},

  year         = {Mon Jul 28 00:00:00 EDT 2014},

  month        = {Mon Jul 28 00:00:00 EDT 2014}

}

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Works referenced in this record:

New tools for structure refinement in PHENIX
journal, August 2010

Adams, Paul; Afonine, Pavel; Echols, Nat
Acta Crystallographica Section A Foundations of Crystallography, Vol. 66, Issue a1
DOI: 10.1107/S010876731009968X

The type III secretion injectisome
journal, November 2006

Cornelis, Guy R.
Nature Reviews Microbiology, Vol. 4, Issue 11
DOI: 10.1038/nrmicro1526

An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination
journal, September 2006

Tobe, T.; Beatson, S. A.; Taniguchi, H.
Proceedings of the National Academy of Sciences, Vol. 103, Issue 40
DOI: 10.1073/pnas.0604891103

Electrostatics of nanosystems: Application to microtubules and the ribosome
journal, August 2001

Baker, N. A.; Sept, D.; Joseph, S.
Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041
DOI: 10.1073/pnas.181342398

The X-ray Crystal Structure of the NF-κB p50·p65 Heterodimer Bound to the Interferon β-κB Site
journal, April 2002

Berkowitz, Benjamin; Huang, De-Bin; Chen-Park, Frances E.
Journal of Biological Chemistry, Vol. 277, Issue 27
DOI: 10.1074/jbc.M200006200

A type III effector protease NleC from enteropathogenic Escherichia coli targets NF-κB for degradation: NleC targets inflammatory signalling
journal, February 2011

Pearson, Jaclyn S.; Riedmaier, Patrice; Marchès, Olivier
Molecular Microbiology, Vol. 80, Issue 1
DOI: 10.1111/j.1365-2958.2011.07568.x

A standard orientation for metallopeptidases
journal, January 2012

Gomis-Rüth, F. Xavier; Botelho, Tiago O.; Bode, Wolfram
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1824, Issue 1
DOI: 10.1016/j.bbapap.2011.04.014

The Apoptogenic Toxin AIP56 Is a Metalloprotease A-B Toxin that Cleaves NF-κb P65
journal, February 2013

Silva, Daniela S.; Pereira, Liliana M. G.; Moreira, Ana R.
PLoS Pathogens, Vol. 9, Issue 2
DOI: 10.1371/journal.ppat.1003128

A novel DNA recognition mode by the NF-κB p65 homodimer
journal, January 1998

Chen, Yong-Qing; Ghosh, Sankar; Ghosh, Gourisankar
Nature Structural Biology, Vol. 5, Issue 1
DOI: 10.1038/nsb0198-67

Proteasome-independent Degradation of Canonical NFκB Complex Components by the NleC Protein of Pathogenic Escherichia coli
journal, February 2011

Mühlen, Sabrina; Ruchaud-Sparagano, Marie-Hélène; Kenny, Brendan
Journal of Biological Chemistry, Vol. 286, Issue 7
DOI: 10.1074/jbc.M110.172254

A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.
journal, February 1995

McDaniel, T. K.; Jarvis, K. G.; Donnenberg, M. S.
Proceedings of the National Academy of Sciences, Vol. 92, Issue 5
DOI: 10.1073/pnas.92.5.1664

Enteropathogenic and enterohaemorrhagic Escherichia coli : more subversive elements
journal, December 1998

Frankel, Gad; Phillips, Alan D.; Rosenshine, Ilan
Molecular Microbiology, Vol. 30, Issue 5
DOI: 10.1046/j.1365-2958.1998.01144.x

Dali server: conservation mapping in 3D
journal, May 2010

Holm, Liisa; Rosenstrï¿½m, Pï¿½ivi
Nucleic Acids Research, Vol. 38, Issue suppl_2
DOI: 10.1093/nar/gkq366

MEROPS: the peptidase database
journal, November 2009

Rawlings, Neil D.; Barrett, Alan J.; Bateman, Alex
Nucleic Acids Research, Vol. 38, Issue suppl_1
DOI: 10.1093/nar/gkp971

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase
journal, July 2013

Cole, A. R.; Ofer, S.; Ryzhenkova, K.
Nucleic Acids Research, Vol. 41, Issue 18
DOI: 10.1093/nar/gkt633

The pathogenic Escherichia coli type III secreted protease NleC degrades the host acetyltransferase p300: NleC targets host p300
journal, August 2011

Shames, Stephanie R.; Bhavsar, Amit P.; Croxen, Matthew A.
Cellular Microbiology, Vol. 13, Issue 10
DOI: 10.1111/j.1462-5822.2011.01640.x

Bacterial Regulation of Intestinal Immune Responses
journal, January 2000

MacDonald, Thomas T.; Pettersson, Sven
Inflammatory Bowel Diseases, Vol. 6, Issue 2
DOI: 10.1097/00054725-200005000-00008

Analysis of the structural consensus of the zinc coordination centers of metalloprotein structures
journal, October 2007

Patel, Kirti; Kumar, Anil; Durani, Susheel
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1774, Issue 10
DOI: 10.1016/j.bbapap.2007.07.010

Attachment of a noninvasive enteric pathogen, enteropathogenic Escherichia coli, to cultured human intestinal epithelial monolayers induces transmigration of neutrophils
journal, November 1996

Savkovic, S. D.; Koutsouris, A.; Hecht, G.
Infection and Immunity, Vol. 64, Issue 11
DOI: 10.1128/iai.64.11.4480-4487.1996

Structure of NF-κB p50/p65 Heterodimer Bound to the PRDII DNA Element from the Interferon-β Promoter
journal, March 2002

Escalante, Carlos R.; Shen, Leyi; Thanos, Dimitris
Structure, Vol. 10, Issue 3
DOI: 10.1016/S0969-2126(02)00723-2

Protein delivery into eukaryotic cells by type III secretion machines
journal, November 2006

Galán, Jorge E.; Wolf-Watz, Hans
Nature, Vol. 444, Issue 7119
DOI: 10.1038/nature05272

Assembly of the Type III Secretion Apparatus of Enteropathogenic Escherichia coli
journal, April 2006

Ogino, Tomoaki; Ohno, Ryuta; Sekiya, Kachiko
Journal of Bacteriology, Vol. 188, Issue 8
DOI: 10.1128/JB.188.8.2801-2811.2006

The κB DNA Sequence from the HIV Long Terminal Repeat Functions as an Allosteric Regulator of HIV Transcription
journal, July 2002

Chen-Park, Frances E.; Huang, De-Bin; Noro, Barbara
Journal of Biological Chemistry, Vol. 277, Issue 27
DOI: 10.1074/jbc.M200007200

Structural Basis of HIV-1 Activation by NF-κB—A Higher-Order Complex of p50:RelA Bound to the HIV-1 LTR
journal, October 2009

Stroud, James C.; Oltman, Amy; Han, Aidong
Journal of Molecular Biology, Vol. 393, Issue 1
DOI: 10.1016/j.jmb.2009.08.023

Solution Structure of a TBP–TAFII230 Complex
journal, September 1998

Liu, Dingjiang; Ishima, Rieko; Tong, Kit I.
Cell, Vol. 94, Issue 5
DOI: 10.1016/S0092-8674(00)81599-8

Protein mimicry of DNA and pathway regulation
journal, December 2005

Putnam, Christopher D.; Tainer, John A.
DNA Repair, Vol. 4, Issue 12
DOI: 10.1016/j.dnarep.2005.08.007

Chaperone release and unfolding of substrates in type III secretion
journal, October 2005

Akeda, Yukihiro; Galán, Jorge E.
Nature, Vol. 437, Issue 7060
DOI: 10.1038/nature03992

Catalytic Domain Architecture of Metzincin Metalloproteases
journal, June 2009

Gomis-Rüth, F. Xavier
Journal of Biological Chemistry, Vol. 284, Issue 23
DOI: 10.1074/jbc.R800069200

Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase 1 1Edited by D. C. Rees
journal, March 1999

Putnam, Christopher D.; Shroyer, Mary Jane N.; Lundquist, Amy J.
Journal of Molecular Biology, Vol. 287, Issue 2
DOI: 10.1006/jmbi.1999.2605

The draft genome sequence of Arsenophonus nasoniae , son-killer bacterium of Nasonia vitripennis , reveals genes associated with virulence and symbiosis
journal, February 2010

Wilkes, T. E.; Darby, A. C.; Choi, J. -H.
Insect Molecular Biology, Vol. 19
DOI: 10.1111/j.1365-2583.2009.00963.x

Attaching and Effacing Bacterial Effector NleC Suppresses Epithelial Inflammatory Responses by Inhibiting NF-κB and p38 Mitogen-Activated Protein Kinase Activation
journal, September 2011

Sham, Ho Pan; Shames, Stephanie R.; Croxen, Matthew A.
Infection and Immunity, Vol. 79, Issue 9
DOI: 10.1128/IAI.05033-11

The Pfam protein families database
journal, November 2011

Punta, M.; Coggill, P. C.; Eberhardt, R. Y.
Nucleic Acids Research, Vol. 40, Issue D1
DOI: 10.1093/nar/gkr1065

High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
journal, July 2013

Anandapadamanaban, Madhanagopal; Andresen, Cecilia; Helander, Sara
Nature Structural & Molecular Biology, Vol. 20, Issue 8
DOI: 10.1038/nsmb.2611

NleC, a Type III Secretion Protease, Compromises NF-κB Activation by Targeting p65/RelA
journal, December 2010

Yen, Hilo; Ooka, Tadasuke; Iguchi, Atsushi
PLoS Pathogens, Vol. 6, Issue 12
DOI: 10.1371/journal.ppat.1001231

Expanded Roles for Multicargo and Class 1B Effector Chaperones in Type III Secretion
journal, May 2012

Thomas, N. A.; Ma, I.; Prasad, M. E.
Journal of Bacteriology, Vol. 194, Issue 15
DOI: 10.1128/JB.00406-12

Structure of Ocr from Bacteriophage T7, a Protein that Mimics B-Form DNA
journal, January 2002

Walkinshaw, M. D.; Taylor, P.; Sturrock, S. S.
Molecular Cell, Vol. 9, Issue 1
DOI: 10.1016/S1097-2765(02)00435-5

Enteropathogenic Escherichia coli
journal, October 1992

Donnenberg, M. S.; Kaper, J. B.
Infection and Immunity, Vol. 60, Issue 10
DOI: 10.1128/iai.60.10.3953-3961.1992

3D structure of EspA filaments from enteropathogenic Escherichia coli: 3D structure of EspA filaments
journal, June 2003

Daniell, Sarah J.; Kocsis, Eva; Morris, Edward
Molecular Microbiology, Vol. 49, Issue 2
DOI: 10.1046/j.1365-2958.2003.03555.x

Convergent evolution: the need to be explicit
journal, January 1994

Doolittle, Russell F.
Trends in Biochemical Sciences, Vol. 19, Issue 1
DOI: 10.1016/0968-0004(94)90167-8

Comparison of the structures of carboxypeptidase A and thermolysin.
journal, November 1977

Kester, W. R.; Matthews, B. W.
Journal of Biological Chemistry, Vol. 252, Issue 21
DOI: 10.1016/S0021-9258(17)41025-8

Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements: EHEC and EHEC T3SS effectors
journal, May 2011

Wong, Alexander R. C.; Pearson, Jaclyn S.; Bright, Michael D.
Molecular Microbiology, Vol. 80, Issue 6
DOI: 10.1111/j.1365-2958.2011.07661.x

Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast
journal, May 2000

Mossessova, Elena; Lima, Christopher D.
Molecular Cell, Vol. 5, Issue 5
DOI: 10.1016/S1097-2765(00)80326-3

Enteropathogenic Escherichia coli (EPEC) inactivate innate immune responses prior to compromising epithelial barrier function
journal, August 2007

Ruchaud-Sparagano, Marie-Hélène; Maresca, Marc; Kenny, Brendan
Cellular Microbiology, Vol. 9, Issue 8
DOI: 10.1111/j.1462-5822.2007.00923.x

An improved SUMO fusion protein system for effective production of native proteins
journal, July 2008

Lee, Chien-Der; Sun, Hui-Chien; Hu, Su-Ming
Protein Science, Vol. 17, Issue 7
DOI: 10.1110/ps.035188.108

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Title: The Structure and Specificity of the Type III Secretion System Effector NleC Suggest a DNA Mimicry Mechanism of Substrate Recognition

Citation Formats

New tools for structure refinement in PHENIX journal, August 2010

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Electrostatics of nanosystems: Application to microtubules and the ribosome journal, August 2001

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A type III effector protease NleC from enteropathogenic Escherichia coli targets NF-κB for degradation: NleC targets inflammatory signalling journal, February 2011

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A novel DNA recognition mode by the NF-κB p65 homodimer journal, January 1998

Proteasome-independent Degradation of Canonical NFκB Complex Components by the NleC Protein of Pathogenic Escherichia coli journal, February 2011

A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. journal, February 1995

Enteropathogenic and enterohaemorrhagic Escherichia coli : more subversive elements journal, December 1998

Dali server: conservation mapping in 3D journal, May 2010

MEROPS: the peptidase database journal, November 2009

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase journal, July 2013

The pathogenic Escherichia coli type III secreted protease NleC degrades the host acetyltransferase p300: NleC targets host p300 journal, August 2011

Bacterial Regulation of Intestinal Immune Responses journal, January 2000

Analysis of the structural consensus of the zinc coordination centers of metalloprotein structures journal, October 2007

Attachment of a noninvasive enteric pathogen, enteropathogenic Escherichia coli, to cultured human intestinal epithelial monolayers induces transmigration of neutrophils journal, November 1996

Structure of NF-κB p50/p65 Heterodimer Bound to the PRDII DNA Element from the Interferon-β Promoter journal, March 2002

Protein delivery into eukaryotic cells by type III secretion machines journal, November 2006

Assembly of the Type III Secretion Apparatus of Enteropathogenic Escherichia coli journal, April 2006

The κB DNA Sequence from the HIV Long Terminal Repeat Functions as an Allosteric Regulator of HIV Transcription journal, July 2002

Structural Basis of HIV-1 Activation by NF-κB—A Higher-Order Complex of p50:RelA Bound to the HIV-1 LTR journal, October 2009

Solution Structure of a TBP–TAFII230 Complex journal, September 1998

Protein mimicry of DNA and pathway regulation journal, December 2005

Chaperone release and unfolding of substrates in type III secretion journal, October 2005

Catalytic Domain Architecture of Metzincin Metalloproteases journal, June 2009

Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase 1 1Edited by D. C. Rees journal, March 1999

The draft genome sequence of Arsenophonus nasoniae , son-killer bacterium of Nasonia vitripennis , reveals genes associated with virulence and symbiosis journal, February 2010

Attaching and Effacing Bacterial Effector NleC Suppresses Epithelial Inflammatory Responses by Inhibiting NF-κB and p38 Mitogen-Activated Protein Kinase Activation journal, September 2011

The Pfam protein families database journal, November 2011

High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation journal, July 2013

NleC, a Type III Secretion Protease, Compromises NF-κB Activation by Targeting p65/RelA journal, December 2010

Expanded Roles for Multicargo and Class 1B Effector Chaperones in Type III Secretion journal, May 2012

Structure of Ocr from Bacteriophage T7, a Protein that Mimics B-Form DNA journal, January 2002

Enteropathogenic Escherichia coli journal, October 1992

3D structure of EspA filaments from enteropathogenic Escherichia coli: 3D structure of EspA filaments journal, June 2003

Convergent evolution: the need to be explicit journal, January 1994

Comparison of the structures of carboxypeptidase A and thermolysin. journal, November 1977

Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements: EHEC and EHEC T3SS effectors journal, May 2011

Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast journal, May 2000

Enteropathogenic Escherichia coli (EPEC) inactivate innate immune responses prior to compromising epithelial barrier function journal, August 2007

An improved SUMO fusion protein system for effective production of native proteins journal, July 2008

New tools for structure refinement in PHENIX
journal, August 2010

The type III secretion injectisome
journal, November 2006

An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination
journal, September 2006

Electrostatics of nanosystems: Application to microtubules and the ribosome
journal, August 2001

The X-ray Crystal Structure of the NF-κB p50·p65 Heterodimer Bound to the Interferon β-κB Site
journal, April 2002

A type III effector protease NleC from enteropathogenic Escherichia coli targets NF-κB for degradation: NleC targets inflammatory signalling
journal, February 2011

A standard orientation for metallopeptidases
journal, January 2012

The Apoptogenic Toxin AIP56 Is a Metalloprotease A-B Toxin that Cleaves NF-κb P65
journal, February 2013

A novel DNA recognition mode by the NF-κB p65 homodimer
journal, January 1998

Proteasome-independent Degradation of Canonical NFκB Complex Components by the NleC Protein of Pathogenic Escherichia coli
journal, February 2011

A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.
journal, February 1995

Enteropathogenic and enterohaemorrhagic Escherichia coli : more subversive elements
journal, December 1998

Dali server: conservation mapping in 3D
journal, May 2010

MEROPS: the peptidase database
journal, November 2009

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase
journal, July 2013

The pathogenic Escherichia coli type III secreted protease NleC degrades the host acetyltransferase p300: NleC targets host p300
journal, August 2011

Bacterial Regulation of Intestinal Immune Responses
journal, January 2000

Analysis of the structural consensus of the zinc coordination centers of metalloprotein structures
journal, October 2007

Attachment of a noninvasive enteric pathogen, enteropathogenic Escherichia coli, to cultured human intestinal epithelial monolayers induces transmigration of neutrophils
journal, November 1996

Structure of NF-κB p50/p65 Heterodimer Bound to the PRDII DNA Element from the Interferon-β Promoter
journal, March 2002

Protein delivery into eukaryotic cells by type III secretion machines
journal, November 2006

Assembly of the Type III Secretion Apparatus of Enteropathogenic Escherichia coli
journal, April 2006

The κB DNA Sequence from the HIV Long Terminal Repeat Functions as an Allosteric Regulator of HIV Transcription
journal, July 2002

Structural Basis of HIV-1 Activation by NF-κB—A Higher-Order Complex of p50:RelA Bound to the HIV-1 LTR
journal, October 2009

Solution Structure of a TBP–TAFII230 Complex
journal, September 1998

Protein mimicry of DNA and pathway regulation
journal, December 2005

Chaperone release and unfolding of substrates in type III secretion
journal, October 2005

Catalytic Domain Architecture of Metzincin Metalloproteases
journal, June 2009

Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase 1 1Edited by D. C. Rees
journal, March 1999

The draft genome sequence of Arsenophonus nasoniae , son-killer bacterium of Nasonia vitripennis , reveals genes associated with virulence and symbiosis
journal, February 2010

Attaching and Effacing Bacterial Effector NleC Suppresses Epithelial Inflammatory Responses by Inhibiting NF-κB and p38 Mitogen-Activated Protein Kinase Activation
journal, September 2011

The Pfam protein families database
journal, November 2011

High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
journal, July 2013

NleC, a Type III Secretion Protease, Compromises NF-κB Activation by Targeting p65/RelA
journal, December 2010

Expanded Roles for Multicargo and Class 1B Effector Chaperones in Type III Secretion
journal, May 2012

Structure of Ocr from Bacteriophage T7, a Protein that Mimics B-Form DNA
journal, January 2002

Enteropathogenic Escherichia coli
journal, October 1992

3D structure of EspA filaments from enteropathogenic Escherichia coli: 3D structure of EspA filaments
journal, June 2003

Convergent evolution: the need to be explicit
journal, January 1994

Comparison of the structures of carboxypeptidase A and thermolysin.
journal, November 1977

Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements: EHEC and EHEC T3SS effectors
journal, May 2011

Ulp1-SUMO Crystal Structure and Genetic Analysis Reveal Conserved Interactions and a Regulatory Element Essential for Cell Growth in Yeast
journal, May 2000

Enteropathogenic Escherichia coli (EPEC) inactivate innate immune responses prior to compromising epithelial barrier function
journal, August 2007

An improved SUMO fusion protein system for effective production of native proteins
journal, July 2008