A Comparison of Nannochloropsis salina Growth Performance in Two Outdoor Pond Designs: Conventional Raceways versus the ARID Pond with Superior Temperature Management
Abstract
The present study examines how climatic conditions and pond design affect the growth performance of microalgae. From January to April of 2011, outdoor batch cultures of Nannochloropsis salina were grown in three replicate 780 L conventional raceways, as well as in an experimental 7500 L algae raceway integrated design (ARID) pond. The ARID culture system utilizes a series of 8-20 cm deep basins and a 1.5 m deep canal to enhance light exposure and mitigate temperature variations and extremes. The ARID culture reached the stationary phase 27 days earlier than the conventional raceways, which can be attributed to its superior temperature management and shallower basins. On a night when the air temperature dropped to -9°C, the water temperature was 18°C higher in the ARID pond than in the conventional raceways. Lipid and fatty acid content ranged from 16 to 25% and from 5 to15%, respectively, as a percentage of AFDW. Palmitic, palmitoleic, and eicosapentaenoic acids comprised the majority of fatty acids. While the ARID culture system achieved nearly double the volumetric productivity relative to the conventional raceways (0.023 versus 0.013 g L-1day-1), areal biomass productivities were of similar magnitude in both pond systems (3.47 versus 3.34 g m-2day-1), suggesting thatmore »
- Authors:
-
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Univ. of Arizona, Tucson, AZ (United States) Dept. of Agriculture and Biosystems Engineering
- Univ. of Arizona, Tucson, AZ (United States) Dept. of Chemical and Environmental Engineering
- Univ. of Arizona, Tucson, AZ (United States) College of Agriculture
- Univ. of Arizona, Tucson, AZ (United States) Dept. of Chemitstry and Biochemistry
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1093708
- Report Number(s):
- DOE-DANF-0003046-Q6
Journal ID: ISSN 1687-806X; 28302-Q
- Grant/Contract Number:
- EE0003046
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Chemical Engineering
- Additional Journal Information:
- Journal Volume: 2012; Journal ID: ISSN 1687-806X
- Publisher:
- Hindawi
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; MICROALGAE; RACEWAY; NANNOCHLOROPSIS SALINA; OUTDOOR CULTIVATION; MASS CULTURE MICROALGE
Citation Formats
Crowe, Braden, Attalah, Said, Agrawal, Shweta, Waller, Peter, Ryan, Randy, Van Wagenen, Jon, Chavis, Aaron, Kyndt, John, Kacira, Murat, Ogden, Kim L., and Huesemann, Michael. A Comparison of Nannochloropsis salina Growth Performance in Two Outdoor Pond Designs: Conventional Raceways versus the ARID Pond with Superior Temperature Management. United States: N. p., 2012.
Web. doi:10.1155/2012/920608.
Crowe, Braden, Attalah, Said, Agrawal, Shweta, Waller, Peter, Ryan, Randy, Van Wagenen, Jon, Chavis, Aaron, Kyndt, John, Kacira, Murat, Ogden, Kim L., & Huesemann, Michael. A Comparison of Nannochloropsis salina Growth Performance in Two Outdoor Pond Designs: Conventional Raceways versus the ARID Pond with Superior Temperature Management. United States. https://doi.org/10.1155/2012/920608
Crowe, Braden, Attalah, Said, Agrawal, Shweta, Waller, Peter, Ryan, Randy, Van Wagenen, Jon, Chavis, Aaron, Kyndt, John, Kacira, Murat, Ogden, Kim L., and Huesemann, Michael. Sun .
"A Comparison of Nannochloropsis salina Growth Performance in Two Outdoor Pond Designs: Conventional Raceways versus the ARID Pond with Superior Temperature Management". United States. https://doi.org/10.1155/2012/920608. https://www.osti.gov/servlets/purl/1093708.
@article{osti_1093708,
title = {A Comparison of Nannochloropsis salina Growth Performance in Two Outdoor Pond Designs: Conventional Raceways versus the ARID Pond with Superior Temperature Management},
author = {Crowe, Braden and Attalah, Said and Agrawal, Shweta and Waller, Peter and Ryan, Randy and Van Wagenen, Jon and Chavis, Aaron and Kyndt, John and Kacira, Murat and Ogden, Kim L. and Huesemann, Michael},
abstractNote = {The present study examines how climatic conditions and pond design affect the growth performance of microalgae. From January to April of 2011, outdoor batch cultures of Nannochloropsis salina were grown in three replicate 780 L conventional raceways, as well as in an experimental 7500 L algae raceway integrated design (ARID) pond. The ARID culture system utilizes a series of 8-20 cm deep basins and a 1.5 m deep canal to enhance light exposure and mitigate temperature variations and extremes. The ARID culture reached the stationary phase 27 days earlier than the conventional raceways, which can be attributed to its superior temperature management and shallower basins. On a night when the air temperature dropped to -9°C, the water temperature was 18°C higher in the ARID pond than in the conventional raceways. Lipid and fatty acid content ranged from 16 to 25% and from 5 to15%, respectively, as a percentage of AFDW. Palmitic, palmitoleic, and eicosapentaenoic acids comprised the majority of fatty acids. While the ARID culture system achieved nearly double the volumetric productivity relative to the conventional raceways (0.023 versus 0.013 g L-1day-1), areal biomass productivities were of similar magnitude in both pond systems (3.47 versus 3.34 g m-2day-1), suggesting that the ARID pond design has to be further optimized, most likely by increasing the culture depth or operating at higher cell densities while maintaining adequate mixing.},
doi = {10.1155/2012/920608},
journal = {International Journal of Chemical Engineering},
number = ,
volume = 2012,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2012},
month = {Sun Jan 01 00:00:00 EST 2012}
}
Works referenced in this record:
Factors affecting lipid accumulation by Nannochloropsis oculata in a two-stage cultivation process
journal, October 2010
- Su, Chia-Hung; Chien, Liang-Jung; Gomes, James
- Journal of Applied Phycology, Vol. 23, Issue 5
As integrated culture system for outdoor production of microalgae and cyanobacteria
journal, January 1997
- Pushparaj, Benjamin; Pelosi, Elio; Tredici, Mario R.
- Journal of Applied Phycology, Vol. 9, Issue 2, p. 113-119
Studies of Marine Planktonic Diatoms: i. Cyclotella nana Hustedt, and Detonula Confervacea (Cleve) Gran.
journal, April 1962
- Guillard, Robert R. L.; Ryther, John H.
- Canadian Journal of Microbiology, Vol. 8, Issue 2
Biomass Productivities in Wild Type and Pigment Mutant of Cyclotella sp. (Diatom)
journal, July 2008
- Huesemann, Michael H.; Hausmann, Tom S.; Bartha, Richard
- Applied Biochemistry and Biotechnology, Vol. 157, Issue 3
A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8
Lipid and biomass production by the halotolerant microalga Nannochloropsis salina
journal, January 1987
- Boussiba, Sammy; Vonshak, Avigad; Cohen, Zvi
- Biomass, Vol. 12, Issue 1
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
journal, May 2008
- Hu, Qiang; Sommerfeld, Milton; Jarvis, Eric
- The Plant Journal, Vol. 54, Issue 4
Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration
journal, June 2006
- Hu, Hanhua; Gao, Kunshan
- Biotechnology Letters, Vol. 28, Issue 13
Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor
journal, January 2009
- Rodolfi, Liliana; Chini Zittelli, Graziella; Bassi, Niccolò
- Biotechnology and Bioengineering, Vol. 102, Issue 1
Effect of light intensity on the proximate biochemical and fatty acid composition of Isochrysis sp. and Nannochloropsis oculata for use in tropical aquaculture
journal, March 1991
- Renaud, S. M.; Parry, D. L.; Thinh, Luong-Van
- Journal of Applied Phycology, Vol. 3, Issue 1
Regulation of Fatty acid Composition by Irradiance Level in the Eustigmatophyte Nannochloropsis sp.1
journal, December 1989
- Sukenik, Assaf; Carmeli, Yael; Berner, Tamar
- Journal of Phycology, Vol. 25, Issue 4
The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp.
journal, March 2011
- Pal, Dipasmita; Khozin-Goldberg, Inna; Cohen, Zvi
- Applied Microbiology and Biotechnology, Vol. 90, Issue 4
Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production
journal, June 2009
- Converti, Attilio; Casazza, Alessandro A.; Ortiz, Erika Y.
- Chemical Engineering and Processing: Process Intensification, Vol. 48, Issue 6
The Biochemical Composition of Marine Microalgae from the Class Eustigma Tophyceae1
journal, February 1993
- Volkman, John K.; Brown, Malcolm R.; Dunstan, Graeme A.
- Journal of Phycology, Vol. 29, Issue 1
Concerns over the reporting of inconsistent data on fatty acid composition for microalgae of the genus Nannochloropsis (Eustigmatophyceae)
journal, February 2011
- Khozin-Goldberg, Inna; Boussiba, Sammy
- Journal of Applied Phycology, Vol. 23, Issue 5
Patterns of variation in the lipid class and fatty acid composition of Nannochloropsis oculata (Eustigmatophyceae) during batch culture: I. The growth cycle
journal, June 1991
- Hodgson, Paul A.; Henderson, R. James; Sargent, John R.
- Journal of Applied Phycology, Vol. 3, Issue 2
ALTERATIONS IN LIPID MOLECULAR SPECIES OF THE MARINE EUSTIGMA TOPHYTE NANNOCHLOROSIS SP. 1
journal, October 1993
- Sukenik, Assaf; Yamaguchi, Yuji; Livne, Alexander
- Journal of Phycology, Vol. 29, Issue 5
Biotechnology of algal biomass production: a review of systems for outdoor mass culture
journal, December 1993
- Chaumont, Daniel
- Journal of Applied Phycology, Vol. 5, Issue 6
Biochemical quality of marine unicellular algae with special emphasis on lipid composition. II. Nannochloropsis sp.
journal, November 1993
- Sukenik, Assaf; Zmora, Odi; Carmeli, Yael
- Aquaculture, Vol. 117, Issue 3-4
Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production
journal, June 2009
- Converti, Attilio; Casazza, Alessandro A.; Ortiz, Erika Y.
- Chemical Engineering and Processing: Process Intensification, Vol. 48, Issue 6
ALTERATIONS IN LIPID MOLECULAR SPECIES OF THE MARINE EUSTIGMA TOPHYTE NANNOCHLOROSIS SP. 1
journal, October 1993
- Sukenik, Assaf; Yamaguchi, Yuji; Livne, Alexander
- Journal of Phycology, Vol. 29, Issue 5
Works referencing / citing this record:
Technologies for Biofuel Production: Current Development, Challenges, and Future Prospects
book, January 2019
- Kour, Divjot; Rana, Kusam Lata; Yadav, Neelam
- Biofuel and Biorefinery Technologies
Minimising losses to predation during microalgae cultivation
journal, March 2017
- Flynn, Kevin J.; Kenny, Philip; Mitra, Aditee
- Journal of Applied Phycology, Vol. 29, Issue 4
Physiology limits commercially viable photoautotrophic production of microalgal biofuels
journal, July 2017
- Kenny, Philip; Flynn, Kevin J.
- Journal of Applied Phycology, Vol. 29, Issue 6
Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana
journal, October 2018
- Steichen, Seth A.; Brown, Judith K.
- Journal of Applied Phycology, Vol. 31, Issue 2
Dependency of Microalgal Production on Biomass and the Relationship to Yield and Bioreactor Scale-up for Biofuels: a Statistical Analysis of 60+ Years of Algal Bioreactor Data
journal, August 2016
- Granata, Tim
- BioEnergy Research, Vol. 10, Issue 1
Response surface methodology as a decision-making tool for optimization of culture conditions of green microalgae Chlorella spp. for biodiesel production
journal, December 2013
- Kirrolia, Anita; Bishnoi, Narsi R.; Singh, Rajesh
- Annals of Microbiology, Vol. 64, Issue 3
Detailed design and optimization of a sustainable micro-algal biofuel process plant
journal, February 2018
- Eloka-Eboka, Andrew; Onunka, Chiemela; Inambao, Freddie
- International Journal of Low-Carbon Technologies, Vol. 13, Issue 2
Dependency Of Microalgal Production On Biomass And The Relationship To Yield And Bioreactor Scale-Up For Biofuels: A Statistical Analysis Of 60+ Years Of Algal Bioreactor Data
text, January 2016
- Granata, Timothy (Autor/in)
- Zenodo