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Title: Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*

Microalgae hold promise for yielding a biofuel feedstock that is sustainable, carbon-neutral, distributed, and only minimally disruptive for the production of food and feed by traditional agriculture. Amongst oleaginous eukaryotic algae, the B race of Botryococcus braunii is unique in that it produces large amounts of liquid hydrocarbons of terpenoid origin. These are comparable to fossil crude oil, and are sequestered outside the cells in a communal extracellular polymeric matrix material. The biosynthetic engineering of terpenoid bio-crude production requires identification of genes and reconstruction of metabolic pathways responsible for production of both hydrocarbons and other metabolites of the alga that compete for photosynthetic carbon and energy.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [3] ;  [2] ;  [1]
  1. Univ. of Arizona, Tucson, AZ (United States)
  2. Univ. of California Los Angeles, Los Angeles, CA (United States)
  3. Texas A & M Univ., College Station, TX (United States)
Publication Date:
OSTI Identifier:
1093677
Grant/Contract Number:
EE0003046
Type:
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 13; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Research Org:
Univ. of Arizona, Tucson, AZ (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS biofuel; terpene biosynthesis; fatty acid biosynthesis; start biosynthesis; transcriptome; autophagy; ABC transporter; botryococcus braunii; botryococcene