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Title: Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices

This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e.,DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d > 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.
Authors:
 [1] ;  [2] ;  [3]
  1. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry; Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
  2. Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
  3. Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering, Dept. of Chemistry and Biochemistry
Publication Date:
OSTI Identifier:
1076433
Grant/Contract Number:
FG02-07ER15851
Type:
Accepted Manuscript
Journal Name:
Biomicrofluidics
Additional Journal Information:
Journal Volume: 3; Journal Issue: 1; Journal ID: ISSN 1932-1058
Publisher:
American Institute of Physics (AIP)
Research Org:
University of Notre Dame, Notre Dame, IN (United States)
Sponsoring Org:
USDOE SC Office of Biological and Environmental Research (SC-23)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 42 ENGINEERING