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Sample records for wv mi vt

  1. VT Nuclear Services ltd | Open Energy Information

    Open Energy Info (EERE)

    VT Nuclear Services ltd Jump to: navigation, search Name: VT Nuclear Services ltd Place: Warrington, United Kingdom Zip: WA4 4BP Sector: Services Product: VT Nuclear Services...

  2. Category:Burlington, VT | Open Energy Information

    Open Energy Info (EERE)

    Burlington VT Central Vermont Pub Serv Corp.png SVMidriseApartment Bur... 68 KB SVQuickServiceRestaurant Burlington VT Central Vermont Pub Serv Corp.png...

  3. Category:Elkins, WV | Open Energy Information

    Open Energy Info (EERE)

    16 files are in this category, out of 16 total. SVFullServiceRestaurant Elkins WV Harrison Rural Elec Assn Inc.png SVFullServiceRestauran... 59 KB SVQuickServiceRestaurant...

  4. DOE - Office of Legacy Management -- Reduction Pilot Plant - WV 01

    Office of Legacy Management (LM)

    Reduction Pilot Plant - WV 01 FUSRAP Considered Sites Site: REDUCTION PILOT PLANT (WV.01 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: International Nickel Company WV.01-1 Location: Cole Street at Alterizer Ave. , Huntington , West Virginia WV.01-2 Evaluation Year: 1987 WV.01-1 Site Operations: Manufactured powdered Nickel for use at Paducah and Portsmouth gaseous diffusion plants and Nickel plated a small quantity of Uranium slugs. WV.01-2

  5. Boynton v. Gilman, 53 Vt. 17 (1880) | Open Energy Information

    Open Energy Info (EERE)

    v. Gilman, 53 Vt. 17 (1880) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Boynton v. Gilman, 53 Vt. 17 (1880)Legal Abstract Riparian rights...

  6. State v. Morse, 84 Vt. 387 (1911) | Open Energy Information

    Open Energy Info (EERE)

    v. Morse, 84 Vt. 387 (1911) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: State v. Morse, 84 Vt. 387 (1911)Legal Hearing State v. Morse, 84...

  7. VA VT CT RI MT WY CO ID UT OR NV CA AZ NM WA TN WV NC AR OK

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 1 Locations of Smart Grid Demonstration and Large-Scale Energy Storage Projects NH 32 Awards Support Projects in 24 States 6 11 MA

  8. VA VT CT RI MT WY CO ID UT OR NV CA AZ NM WA TN WV NC AR OK

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 1 Smart Grid Demonstration Project Locations NH MA 16 Awards Support Projects in 21 States

  9. VA VT CT RI MT WY CO ID UT OR NV CA AZ NM WA TN WV NC AR OK

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7 2 1 Energy Storage Demonstration Project Locations NH 16 Awards Support Projects in 9 States MA

  10. VA VT CT RI MT WY CO ID UT OR NV CA AZ NM WA TN WV NC AR OK

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 1 Locations of Smart Grid Demonstration and Large-Scale Energy Storage Projects NH 32 Awards Support Projects in 24 States 6 11 MA

  11. ARM - Campaign Instrument - mfrsr-wv1mich

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    mich Comments? We would love to hear from you Send us a note below or call us at 1-888-ARM-DATA. Send Campaign Instrument : MFRSR-WV-Michalsky1 (MFRSR-WV1MICH) Instrument...

  12. Vermont Instructions for Preparing the VT Hazardous Waste Handler...

    Open Energy Info (EERE)

    Instructions for Preparing the VT Hazardous Waste Handler Site ID Form Jump to: navigation, search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance -...

  13. RAPID/Roadmap/19-VT-a | Open Energy Information

    Open Energy Info (EERE)

    RAPIDRoadmap19-VT-a < RAPID | Roadmap Jump to: navigation, search RAPID Regulatory and Permitting Information Desktop Toolkit BETA About Bulk Transmission Geothermal...

  14. RAPID/Roadmap/7-VT-a | Open Energy Information

    Open Energy Info (EERE)

    RAPIDRoadmap7-VT-a < RAPID | Roadmap Jump to: navigation, search RAPID Regulatory and Permitting Information Desktop Toolkit BETA About Bulk Transmission Geothermal Hydropower...

  15. RAPID/Roadmap/8-VT-c | Open Energy Information

    Open Energy Info (EERE)

    RAPIDRoadmap8-VT-c < RAPID | Roadmap Jump to: navigation, search RAPID Regulatory and Permitting Information Desktop Toolkit BETA About Bulk Transmission Geothermal Hydropower...

  16. RAPID/Roadmap/1-VT-a | Open Energy Information

    Open Energy Info (EERE)

    RAPIDRoadmap1-VT-a < RAPID | Roadmap Jump to: navigation, search RAPID Regulatory and Permitting Information Desktop Toolkit BETA About Bulk Transmission Geothermal Hydropower...

  17. RAPID/Roadmap/6-VT-a | Open Energy Information

    Open Energy Info (EERE)

    Roadmap6-VT-a < RAPID | Roadmap Jump to: navigation, search RAPID Regulatory and Permitting Information Desktop Toolkit BETA About Bulk Transmission Geothermal Hydropower Solar...

  18. RAPID/Roadmap/13-VT-a | Open Energy Information

    Open Energy Info (EERE)

    RAPIDRoadmap13-VT-a < RAPID | Roadmap Jump to: navigation, search RAPID Regulatory and Permitting Information Desktop Toolkit BETA About Bulk Transmission Geothermal...

  19. RAPID/Roadmap/3-VT-c | Open Energy Information

    Open Energy Info (EERE)

    Flowchart Narrative 3-VT-c.1 - Contact VTrans or Local Area Agency Transportation Maintenance District The developer should contact VTrans Utilities and Permits Unit or a local...

  20. RAPID/Roadmap/12-VT-a | Open Energy Information

    Open Energy Info (EERE)

    Toolkit related to hydropower are currently under development and subject to change ANR Fish and Wildlife Conditions and Recommendations (12-VT-a) The Federal Power Act (FPA)...

  1. DOE - Office of Legacy Management -- Food Machinery and Chemical Co - WV 04

    Office of Legacy Management (LM)

    Food Machinery and Chemical Co - WV 04 FUSRAP Considered Sites Site: Food Machinery and Chemical Co. (WV.04 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Food Machining Corp WV.04-1 Location: Nitro , West Virginia WV.04-1 Evaluation Year: 1994 WV.04-1 Site Operations: Proposed research and development on treatment of calcium and aluminum phosphate materials to extract uranium. WV.04-2 WV.04-3 Site Disposition: Eliminated - Operations were

  2. North Troy, VT Natural Gas Pipeline Imports From Canada (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Million Cubic Feet) North Troy, VT Natural Gas Pipeline Imports From Canada (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's ...

  3. North Troy, VT Natural Gas Pipeline Imports From Canada (Dollars...

    U.S. Energy Information Administration (EIA) Indexed Site

    Dollars per Thousand Cubic Feet) North Troy, VT Natural Gas Pipeline Imports From Canada (Dollars per Thousand Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 ...

  4. ARM - Campaign Instrument - mfrsr-wv1barn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    barn Comments? We would love to hear from you Send us a note below or call us at 1-888-ARM-DATA. Send Campaign Instrument : MFRSR Water-Vapor Barnard Algorithm (MFRSR-WV1BARN)...

  5. DOE - Office of Legacy Management -- Morgantown Ordnance Works - WV 03

    Office of Legacy Management (LM)

    Morgantown Ordnance Works - WV 03 FUSRAP Considered Sites Site: Morgantown Ordnance Works (WV.03 ) Regulated by the U.S. Environmental Protection Agency. More information at http://cumulis.epa.gov/supercpad/cursites/csitinfo.cfm?id=0302884&msspp=med Designated Name: Not Designated under FUSRAP Alternate Name: Ordnance Works Disposal Areas Location: Monongalia County, West Virginia Evaluation Year: Not considered for FUSRAP - in another program Site Operations: Industrial Site Disposition:

  6. DOE - Office of Legacy Management -- The Carborundum Co Inc - WV 02

    Office of Legacy Management (LM)

    - WV 02 FUSRAP Considered Sites Site: THE CARBORUNDUM CO., INC (WV.02 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: AMAX Inc WV.02-1 Location: Wood County , West Virginia WV.02-1 Evaluation Year: 1982 WV.02-1 Site Operations: Produced high-grade Zirconium metal for use in construction of nuclear reactors for the Navy circa late-1950s and 1960s; Conducted small scale Zirconium and Uranium testing in the mid-1970s. WV.02-2 Site Disposition:

  7. Davis v. Fuller, 12 Vt. 178, 198 (1840) | Open Energy Information

    Open Energy Info (EERE)

    v. Fuller, 12 Vt. 178, 198 (1840) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Davis v. Fuller, 12 Vt. 178, 198 (1840)Legal Hearing Davis...

  8. Magoon v. Harris, 46 Vt. 264, 269 (1863) | Open Energy Information

    Open Energy Info (EERE)

    Magoon v. Harris, 46 Vt. 264, 269 (1863) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Magoon v. Harris, 46 Vt. 264, 269 (1863)Legal...

  9. Lawrie v. Silsby, 76 Vt. 240, 253 (1904) | Open Energy Information

    Open Energy Info (EERE)

    Lawrie v. Silsby, 76 Vt. 240, 253 (1904) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Lawrie v. Silsby, 76 Vt. 240, 253 (1904)Legal...

  10. Chatfield v. Wilson, 31 Vt. 358, 262-63 (1858) | Open Energy...

    Open Energy Info (EERE)

    Chatfield v. Wilson, 31 Vt. 358, 262-63 (1858) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Chatfield v. Wilson, 31 Vt. 358, 262-63...

  11. Johns v. Stevens, 3 Vt. 308, 315-16 (1830) | Open Energy Information

    Open Energy Info (EERE)

    v. Stevens, 3 Vt. 308, 315-16 (1830) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Johns v. Stevens, 3 Vt. 308, 315-16 (1830)Legal Abstract...

  12. Snow v. Parsons, 28 Vt. 459, 460-64 (1856) | Open Energy Information

    Open Energy Info (EERE)

    v. Parsons, 28 Vt. 459, 460-64 (1856) Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal CaseHearing: Snow v. Parsons, 28 Vt. 459, 460-64 (1856)Legal...

  13. Highgate Springs, VT Natural Gas Liquefied Natural Gas Imports (Million

    U.S. Energy Information Administration (EIA) Indexed Site

    Cubic Feet) (Million Cubic Feet) Highgate Springs, VT Natural Gas Liquefied Natural Gas Imports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 109 2014 41 23 2015 46 39 34 41 41 39 40 41 43 37 2016 41 38 43 55 110 112 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Imports by Point of

  14. Highgate Springs, VT Natural Gas Pipeline Imports From Canada (Million

    Gasoline and Diesel Fuel Update (EIA)

    Cubic Feet) (Million Cubic Feet) Highgate Springs, VT Natural Gas Liquefied Natural Gas Imports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 109 2014 41 23 2015 46 39 34 41 41 39 40 41 43 37 2016 41 38 43 55 110 112 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Imports by Point of

  15. West Virginia Smart Grid Implementation Plan (WV SGIP) Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    West Virginia Smart Grid Implementation Plan (WV SGIP) Project APERC Report on Assessment of As-Is Grid by Non-Utility Stakeholders Introduction One goal of this grid modernization project is to assess the current status of the electric power grid in West Virginia in order to define the potential to implement smart grid technologies. Thus, an initial task of this project was to define the current state or "As-Is" grid in West Virginia. Financial and time constraints prohibited the

  16. Duration Test Report for the Ventera VT10 Wind Turbine

    SciTech Connect (OSTI)

    Smith, J.; Huskey, A.; Jager, D.; Hur, J.

    2013-06-01

    This project was established to help reduce the barriers of wind energy expansion by providing independent testing results for small wind turbines. Five turbines were tested at the National Wind Technology Center (NWTC) at the National Renewable Energy Laboratory (NREL) as a part of round one of this project. Duration testing is one of up to five tests that may be performed on the turbines, including power performance, safety and function, noise, and power quality. Test results will provide manufacturers with reports that can be used to fulfill part of the requirements for small wind turbine certification. The test equipment included a grid-connected Ventera Energy Corporation VT10 wind turbine mounted on an 18.3-m (60-ft) self-supporting lattice tower manufactured by Rohn.

  17. Hawley v. Sheldon, 64 Vt. 491, 493-94 (1892) | Open Energy Information

    Open Energy Info (EERE)

    as a "distinct channel...with well-defined banks, cut through the turf, and into the soil by the flowing of the water..." Hawley v. Sheldon, 64 Vt. 491, 493-94 (1892). Hearing...

  18. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Joshua Hull Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-0906 joshua.hull@netl.doe.gov Dr. Brenda Bowen Principal Investigator Associate Director, Global Change and Sustainability Center Associate Research Professor, Geology and Geophysics

  19. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CONTACTS Traci Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Andrea McNemar Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-2024 andrea.mcnemar@netl.doe.gov Ruben Juanes Principal Investigator Massachusetts Institute of Technology 77 Massachusetts Avenue Room 48-319 Cambridge, MA 02139

  20. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    GSRA CONTACTS Traci Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Andrea McNemar Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-2024 andrea.mcnemar@netl.doe.gov Constantin Cranganu Principal Investigator Brooklyn College 2900 Bedford Avenue 4415 Ingersoll Hall Brooklyn, NY 11210 718-951-5000

  1. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CONTACTS Traci Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road PO Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Joshua Hull Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P. O. Box 880 Morgantown, WV 26507-0880 304-285-0906 joshua.hull@netl.doe.gov William Lawson Principal Investigator Petroleum Technology Transfer Council P.O. Box 8531 Tulsa, OK 74101-8531 918-629-1056 wlawson@appg.org

  2. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    GSRA CONTACTS Traci Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Darin Damiani Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-4398 darin.damiani@netl.doe.gov Vivak Malhotra Principal Investigator Southern Illinois University Neckers 483A Mailcode: 4401 Carbondale, IL 62901 618-453-2643 Fax:

  3. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Darin Damiani Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-4398 darin.damiani@netl.doe.gov Robert J. Finley Principal Investigator Illinois State Geological Survey 615 E. Peabody Drive Champaign, IL 61820 217-244-8389 finley@illinois.edu PARTNERS Ameren American Air

  4. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Program Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Dawn Deel Project Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507 304-285-4133 dawn.deel@netl.doe.gov Sherry Mediati Business Contact California Energy Commission 1516 9th Street, MS 1 Sacramento, CA 95814 916-654-4204 smediati@energy.state.ca.us Mike Gravely Principal

  5. Wind Turbine Generator System Safety and Function Test Report for the Ventera VT10 Wind Turbine

    SciTech Connect (OSTI)

    Smith, J.; Huskey, A.; Jager, D.; Hur, J.

    2012-11-01

    This report summarizes the results of a safety and function test that NREL conducted on the Ventera VT10 wind turbine. This test was conducted in accordance with the International Electrotechnical Commissions' (IEC) standard, Wind Turbine Generator System Part 2: Design requirements for small wind turbines, IEC 61400-2 Ed.2.0, 2006-03.

  6. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    SciTech Connect (OSTI)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  7. Transportation Energy Data Book, Vehicle Technologies Market Report, and VT Fact of the Week

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transportation Data Programs: Transportation Energy Data Book, Vehicle Technologies Market Report, and VT Fact of the Week This presentation does not contain any proprietary, confidential, or otherwise restricted information Project ID # VAN009 2015 U.S. DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting June 8-12, 2015 Principal Investigator: Stacy Davis June 11, 2015 2 Overview * Project start date: October 2014 * Project end

  8. Scoping Study for Demand Respose DFT II Project in Morgantown, WV

    SciTech Connect (OSTI)

    Lu, Shuai; Kintner-Meyer, Michael CW

    2008-06-06

    This scoping study describes the underlying data resources and an analysis tool for a demand response assessment specifically tailored toward the needs of the Modern Grid Initiatives Demonstration Field Test in Phase II in Morgantown, WV. To develop demand response strategies as part of more general distribution automation, automated islanding and feeder reconfiguration schemes, an assessment of the demand response resource potential is required. This report provides the data for the resource assessment for residential customers and describes a tool that allows the analyst to estimate demand response in kW for each hour of the day, by end-use, season, day type (weekday versus weekend) with specific saturation rates of residential appliances valid for the Morgantown, WV area.

  9. Local Teams from PA, WV Travel to Washington D.C. for National Science Bowl

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    | netl.doe.gov Regional News Local Teams from PA, WV Travel to Washington D.C. for National Science Bowl Pittsburgh, Pa. - The National Energy Technology Laboratory have sent the regional winners of the southwestern Pennsylvania (SWPA) and West Virginia Science Bowls off to compete in the U.S. Department of Energy National Science Bowl April 28-May 2, 2016, in Washington, D.C. By winning their regional tournaments, the Marshall Middle School (Wexford, PA), Morgantown High School (Morgantown,

  10. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Training Center CONTACTS Traci Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road PO Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Andrea Dunn Project Manager National Energy Technology Laboratory 626 Cochrans Mill Road P.O. Box 10940 Pittsburgh, PA 15236 412-386-7594 andrea.dunn@netl.doe.gov Hilary Olson Project Director/Principal Investigator University of Texas at Austin 1 University Station, C0300 Austin, TX

  11. Highgate Springs, VT Natural Gas Liquefied Natural Gas Imports from Canada

    U.S. Energy Information Administration (EIA) Indexed Site

    (Million Cubic Feet) Liquefied Natural Gas Imports from Canada (Million Cubic Feet) Highgate Springs, VT Natural Gas Liquefied Natural Gas Imports from Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 88 139 139 79 109 2014 41 23 2015 46 39 34 41 41 39 40 41 43 37 2016 41 38 43 55 110 112 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date:

  12. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    O G R A M FAC T S Strategic Center for Natural Gas & Oil LOCATION Arctic Energy Office National Energy Technology Laboratory 420 L Street, Suite 305 Anchorage, Alaska 99501-5901 CONTACTS Albert B. Yost II Sr. Management Technical Advisor Strategic Center for Natural Gas & Oil National Energy Technology Laboratory 3610 Collins Ferry Road Morgantown, WV 26507-0880 304-285-4479 albert.yost@netl.doe.gov Maria Vargas Deputy Director Strategic Center for Natural Gas & Oil National Energy

  13. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CONTACTS Traci Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road P.O. Box 880 Morgantown, WV 26507-0880 304-285-1345 traci.rodosta@netl.doe.gov Andrea Dunn Project Manager National Energy Technology Laboratory 626 Cochrans Mill Road P.O. Box 10940 Pittsburgh, PA 15236 412-386-7594 andrea.dunn@netl.doe.gov Marte Gutierrez Principal Investigator Colorado School of Mines 1600 Illinois Street Golden, CO 80401 303-273-3468 Fax: 303-273-3602

  14. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Rodosta Carbon Storage Technology Manager National Energy Technology Laboratory 3610 Collins Ferry Road PO Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Bruce Brown Project Manager National Energy Technology Laboratory 626 Cochrans Mill Road P.O. Box 10940 Pittsburgh, PA 15236 412-386-7313 bruce.brown@netl.doe.gov Kathryn Baskin Principal Investigator Managing Director Southern States Energy Board 6325 Amherst Court Norcross, GA 30092 770-242-7712 baskin@sseb.org PARTNERS

  15. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA * Sugar Land, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PO Box 880 Morgantown, WV 26507 304-285-1345 traci.rodosta@netl.doe.gov Andrea Dunn Project Manager National Energy Technology Laboratory 626 Cochrans Mill Road PO Box 10940 Pittsburgh, PA 15236-0940 412-386-7594 andrea.dunn@netl.doe.gov Charles D. Gorecki Technical Contact Deputy Associate Director for Research Energy & Environmental Research Center University of North Dakota 15 North 23 rd Street, Stop 9018 Grand Forks, ND 58202-9018 701-777-5355 cgorecki@undeerc.org Edward N. Steadman

  16. If you reside in WASHINGTON, DC - MD -VA- WV your salary will range from:

    National Nuclear Security Administration (NNSA)

    If you are employed in the WASHINGTON, DC Metropolitan Area (D.C., Baltimore, Northern VA, Eastern WV, and Southern PA) your salary will range from: Pay Band Pay Plan(s) Minimum Maximum Developmental EN $49,246 $74,872 01 EK/EJ $34,075 $58,511 02 EK/EJ $51,630 $84,855 03 EK/EJ/EN $74,872 $119,238 04 EK/EJ/EN $105,211 $165,300 05 EK/EJ/EN $148,510 $165,300 If you are employed in OAKLAND/LIVERMORE, CA your salary will range from: Pay Band Pay Plan(s) Minimum Maximum Developmental EN $53,579

  17. Transportation Data Programs:Transportation Energy Data Book,Vehicle Technologies Market Report, and VT Fact of the Week

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Data Book, Vehicle Technologies Market Report, and VT Fact of the Week Stacy C. Davis, P.I. Oak Ridge National Laboratory May 16, 2013 This presentation does not contain any proprietary, confidential, or otherwise restricted information Project ID# VAN009 2 ORNL - Stacy C. Davis | Project ID# VAN009 Overview * Project start date: October 2012 * Project end date: September 2013 * Percent complete: 80% * Barriers addressed - Multi-Year Program Plan 2011 - 2015 Section 2.6 Outreach,

  18. F-1 U.S. Energy Information Administration | Annual Energy Outlook...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Central West North Central East North Central Mountain AK WA MT WY ID NV UT CO AZ NM TX OK IA KS MO IL IN KY TN MS AL FL GA SC NC WV PA NJ MD DE NY CT VT ME RI MA NH VA WI MI OH...

  19. Chapter V

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... AL LA GA AR NC NY MI PA IN VA MS TN KY OH ME SC WV MD VT MA NH CT NJ DE R I DC b Coal Fired Power Plant Supplied by the Powder River Basin Powder River Basin 0 220 110 Miles The ...

  20. Real-time sub-<mi>>ngstrom...

    Office of Scientific and Technical Information (OSTI)

    Real-time sub-<mi>>ngstrom imaging of reversible and irreversible conformations in rhodium catalysts and graphene Kisielowski, Christian; Wang,...

  1. Mi GmbH | Open Energy Information

    Open Energy Info (EERE)

    Mi GmbH Jump to: navigation, search Name: Mi GmbH Place: Switzerland Zip: CH-6340 Sector: Solar Product: Baar-based manufacturer and distributor of fruit juices. The firm is also...

  2. miRNAs in brain development

    SciTech Connect (OSTI)

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan

    2014-02-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.

  3. DOE - Office of Legacy Management -- Michigan Velsicol Chemical Corp - MI

    Office of Legacy Management (LM)

    03 Michigan Velsicol Chemical Corp - MI 03 FUSRAP Considered Sites Site: MICHIGAN [VELSICOL] CHEMICAL CORP. (MI.03 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Velsicol Chemical Corp. MI.03-1 Location: St. Louis , Michigan MI.03-2 Evaluation Year: Circa 1987 MI.03-3 Site Operations: Rare earth processing facility. MI.03-2 Site Disposition: Eliminated - No Authority - NRC survey MI.03-3 Radioactive Materials Handled: Yes Primary Radioactive

  4. DOE - Office of Legacy Management -- Star Cutter Corp - MI 15

    Office of Legacy Management (LM)

    Star Cutter Corp - MI 15 FUSRAP Considered Sites Site: STAR CUTTER CORP. (MI.15) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Farmington , Michigan MI.15-1 Evaluation Year: 1991 MI.15-2 Site Operations: Performed a one time uranium slug drilling operation test in 1956. MI.15-3 MI.15-1 Site Disposition: Eliminated - Potential for contamination considered remote based on limited scope and quantity of materials handled MI.15-2 Radioactive

  5. DOE - Office of Legacy Management -- Wolverine Tube Division - MI 05

    Office of Legacy Management (LM)

    Wolverine Tube Division - MI 05 FUSRAP Considered Sites Site: Wolverine Tube Division (MI.05) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Wolverine Tube Division of Calumet & Hecla Consolidated Copper Co. Star Tool Hermes Automotive Manufacturing Corporation MI.05-1 MI.05-2 Location: 1411 Central Avenue , Detroit , Michigan MI.05-3 Evaluation Year: 1990 MI.05-2 Site Operations: 1943 - Conducted research and development of methods for spinning

  6. DOE - Office of Legacy Management -- Adrian - MI 01

    Office of Legacy Management (LM)

    Adrian - MI 01 FUSRAP Considered Sites Adrian, MI Alternate Name(s): Bridgeport Brass Co. Special Metals Extrusion Plant Bridgeport Brass Company General Motors General Motors Company, Adrian MI.01-1 Location: 1450 East Beecher Street, Adrian, Michigan MI.01-3 Historical Operations: Performed uranium extrusion research and development and metal fabrication work for the AEC using uranium, thorium, and plutonium. MI.01-2 Eligibility Determination: Eligible MI.01-1 Radiological Survey(s):

  7. DOE - Office of Legacy Management -- Carboloy Co - MI 12

    Office of Legacy Management (LM)

    Carboloy Co - MI 12 FUSRAP Considered Sites Site: Carboloy Co. (MI.12 ) Eliminated from further consideration under FUSRAP - AEC licensed facility Designated Name: Not Designated Alternate Name: General Electric MI.12-1 Location: 11177 E. Eight Mile Road , Detroit , Michigan MI.12-1 MI.12-2 Evaluation Year: 1987-1991 MI.12-3 MI.12-4 MI.12-6 Site Operations: Turned-down the outer diameter of uranium metal slugs and conducted pilot plant scale operations for hot pressing uranium dioxide pellets

  8. DOE - Office of Legacy Management -- Oliver Corp - MI 11

    Office of Legacy Management (LM)

    Oliver Corp - MI 11 FUSRAP Considered Sites Site: OLIVER CORP. (MI.11 ) Eliminated from further consideration under FUSRAP - Referred to NRC Designated Name: Not Designated Alternate Name: Behnke Warehousing Incorporated MI.11-1 Location: 433 East Michigan Avenue , Battle Creek , Michigan MI.11-1 Evaluation Year: 1986 MI.11-4 Site Operations: Conducted production scale briquetting of green salt and magnesium blend under AEC license Nos. SNM-591, SUB-579, and C-3725. MI.11-1 MI.11-3 Site

  9. The NuMI Neutrino Beam

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; et al

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  10. DOE - Office of Legacy Management -- Detrex Corp - MI 10

    Office of Legacy Management (LM)

    Detrex Corp - MI 10 FUSRAP Considered Sites Site: Detrex Corp. (MI.10 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Detroit , Michigan MI.10-1 Evaluation Year: 1987 MI.10-2 Site Operations: Conducted experimental runs relative to pickling/degreasing of one handful of uranium turnings MI.10-1 Site Disposition: Eliminated - Potential for contamination considered remote due to small quantity of material handled - There is no

  11. Analysis of natural gases, AL, AR, FL, GA, IL, IN, IA, KY, LA, MD, MI, MS, MO, NJ, NY, NC, OH, PA, TN, VA, and WV; 1951-1991 (for microcomputers). Data file

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    The U.S. Bureau of Mines diskette contains analysis and related source data for 2,357 natural gas samples collected from miscellaneous states, which include the following states: Alabama, Arkansas (except Arkoma Basin), Florida, Georgia, Illinois, Indiana, Iowa, Kentucky, Louisiana, Maryland, Michigan, Mississippi, Missouri, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Tennessee, Virginia, and West Virginia. All samples were obtained and analyzed as part of the Bureau's investigations of occurrences of helium in natural gases of countries with free market economies. The survey has been conducted since 1917. The analysis contained on the diskette contain the full range of component analysis data. Five files are on the diskette: READ.ME, MISC.TXT, MISC.DBF, USHEANAL.DBF, and BASINCDE.TXT.

  12. DOE - Office of Legacy Management -- Naval Ordnance Plant - MI...

    Office of Legacy Management (LM)

    Eliminated from further consideration under FUSRAP - Referred to DoD for action Designated ... MI.0-03-1 Site Disposition: Eliminated - No Authority - Referred to DoD MI.0-03-1 ...

  13. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    SciTech Connect (OSTI)

    Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R.; Postier, Russell G.; Brackett, Daniel J.; Schmittgen, Thomas D.

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  14. “Nodal Gap” induced by the incommensurate diagonal spin density modulation in underdoped high- <mi>Tmi>c> superconductors

    SciTech Connect (OSTI)

    Zhou, Tao; Gao, Yi; Zhu, Jian -Xin

    2015-03-07

    Recently it was revealed that the whole Fermi surface is fully gapped for several families of underdoped cuprates. The existence of the finite energy gap along the <mi>d>-wave nodal lines (nodal gap) contrasts the common understanding of the <mi>d>-wave pairing symmetry, which challenges the present theories for the high-<mi>Tmi><mi>c>superconductors. Here we propose that the incommensurate diagonal spin-density-wave order can account for the above experimental observation. The Fermi surface and the local density of states are also studied. Our results are in good agreement with many important experiments in high-<mi>Tmi><mi>c>superconductors.

  15. Characterization of function and regulation of miR-24-1 and miR-31

    SciTech Connect (OSTI)

    Sun Fenyong; Wang Jiayi; Pan Qiuhui; Yu Yongchun; Zhang Yue; Wan Yang; Wang Ju; Li Xiaoyan; Hong An

    2009-03-13

    To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b{approx}27b{approx}24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.

  16. DOE - Office of Legacy Management -- Dow Chemical Co - Midland - MI 06

    Office of Legacy Management (LM)

    Midland - MI 06 FUSRAP Considered Sites Site: Dow Chemical Co. - Midland (MI.06 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Midland , Michigan MI.06-1 Evaluation Year: Circa 1987 MI.06-2 Site Operations: Conducted development work for production of magnesium-thorium alloys. MI.06-1 Site Disposition: Eliminated - AEC licensed site MI.06-1 MI.06-2 Radioactive Materials Handled: Yes Primary Radioactive Materials Handled:

  17. DOE - Office of Legacy Management -- General Motors Co - Flint - MI 07

    Office of Legacy Management (LM)

    Motors Co - Flint - MI 07 FUSRAP Considered Sites Site: GENERAL MOTORS CO. (MI.07 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: A.C. Spark Plug Dort Highway Plant MI.07-1 MI.07-2 Location: Flint , Michigan MI.07-1 Evaluation Year: 1987 MI.07-3 Site Operations: Processed thorium oxide, uranium oxide, and beryllium oxide into crucibles for the Chicago Area. MI.07-3 MI.07-4 MI.07-5 Site Disposition: Eliminated - Potential for contamination

  18. MINOS Experiment and NuMI Beam Home Page

    Broader source: All U.S. Department of Energy (DOE) Office Webpages

    NuMI-MINOS Neutrino Logo NuMI Beamline and MINOS Experiment Neutrino Logo The MINOS Experiment and NuMI Beamline Fermilab Logo MINOS Experiment Links ◊ MINOS for the Public ◊ Scientific Results ◊ MINOS at Work ◊ NuMI at Work ◊ MINOS+ Experiment Fermilab Neutrino Links ◊ Neutrino FAQ ◊ MINOS Underground Areas at Fermilab ◊ PPD Intensity Frontier Dept Back to - - - ◊ Fermilab at Work ◊ Fermilab Home the MINOS Far Detector in the Soudan Mine MINOS collaborators assembling the

  19. Magnetocrystalline anisotropy in <mi>UMn>2<mi>Ge>2 and related Mn-based actinide ferromagnets

    SciTech Connect (OSTI)

    Parker, David S.; Ghimire, Nirmal; Singleton, John; Thompson, J. D.; Bauer, Eric D.; Baumbach, Ryan; Mandrus, David; Li, Ling; Singh, David J.

    2015-05-04

    We present magnetization isotherms in pulsed magnetic fields up to 62 Tesla, supported by first principles calculations, demonstrating a huge uniaxial magnetocrystalline anisotropy energy - approximately 20 MJ/m3 - in <mi>UMn>2<mi>Ge>2. This large anisotropy results from the extremely strong spin-orbit coupling affecting the uranium 5 f electrons, which in the calculations exhibit a substantial orbital moment exceeding 2 μB. Finally, we also find from theoretical calculations that a number of isostructural Mn-actinide compounds are expected to have similarly large anisotropy.

  20. ,"Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    ,"Worksheet Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2014 ,"Release...

  1. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    SciTech Connect (OSTI)

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation of the

  2. miR-92a family and their target genes in tumorigenesis and metastasis

    SciTech Connect (OSTI)

    Li, Molin; Guan, Xingfang; Sun, Yuqiang; Mi, Jun; Shu, Xiaohong; Liu, Fang; Li, Chuangang

    2014-04-15

    The miR-92a family, including miR-25, miR-92a-1, miR-92a-2 and miR-363, arises from three different paralog clusters miR-17-92, miR-106a-363, and miR-106b-25 that are highly conservative in the process of evolution, and it was thought as a group of microRNAs (miRNAs) correlated with endothelial cells. Aberrant expression of miR-92a family was detected in multiple cancers, and the disturbance of miR-92a family was related with tumorigenesis and tumor development. In this review, the progress on the relationship between miR-92a family and their target genes and malignant tumors will be summarized. - Highlights: Aberrant expression of miR-92a, miR-25 and miR-363 can be observed in many kinds of malignant tumors. The expression of miR-92a family is regulated by LOH, epigenetic alteration, transcriptional factors such as SP1, MYC, E2F, wild-type p53 etc. Roles of miR-92a family in tumorigenesis and development: promoting cell proliferation, invasion and metastasis, inhibiting cell apoptosis.

  3. DOE - Office of Legacy Management -- Baker-Perkins Co - MI 13

    Office of Legacy Management (LM)

    Baker-Perkins Co - MI 13 FUSRAP Considered Sites Site: Baker-Perkins Co (MI 13) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Saginaw , Michigan MI.13-1 Evaluation Year: 1991 MI.13-1 MI.13-2 Site Operations: Small scale oxide mixing demonstrations and testing in May, 1956. MI.13-2 Site Disposition: Eliminated - Potential for contamination remote based on limited scope of activities at the site MI.13-3 Radioactive Materials Handled: Yes

  4. DOE - Office of Legacy Management -- Revere Copper and Brass Co - MI 04

    Office of Legacy Management (LM)

    Revere Copper and Brass Co - MI 04 FUSRAP Considered Sites Site: REVERE COPPER AND BRASS CO. ( MI.04 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Revere Copper and Brass MI.04-1 Location: 5851 West Jefferson Street , Detroit , Michigan MI.04-1 Evaluation Year: 1990 MI.04-2 Site Operations: Extrusion of tuballoy rods, myrnalloy rods and beryllium shapes in the 1940s. MI.04-3 MI.04-4 Site Disposition: Eliminated - Radiation levels below criteria

  5. miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression

    SciTech Connect (OSTI)

    Gao, Yong; Luo, Ling-hui; Li, Shuai; Yang, Cao

    2014-02-07

    Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.

  6. MiR-218 Mediates tumorigenesis and metastasis: Perspectives and implications

    SciTech Connect (OSTI)

    Lu, Ying-fei; Zhang, Li; Waye, Mary Miu Yee; Fu, Wei-ming; Zhang, Jin-fang

    2015-05-15

    MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. As a highly conserved miRNA across a variety of species, microRNA-218 (miR-218) was found to play pivotal roles in tumorigenesis and progression. A group of evidence has demonstrated that miR-218 acts as a tumor suppressor by targeting many oncogenes related to proliferation, apoptosis and invasion. In this review, we provide a complex overview of miR-218, including its regulatory mechanisms, known functions in cancer and future challenges as a potential therapeutic target in human cancers. - Highlights: • miR-218 is frequently down regulated in multiple cancers. • miR-218 plays pivotal roles in carcinogenesis. • miR-218 mediates proliferation, apoptosis, metastasis, invasion, etc. • miR-218 mediates tumorigenesis and metastasis via multiple pathways.

  7. padd map

    U.S. Energy Information Administration (EIA) Indexed Site

    FL PADD 4: Rocky Mountain PADD 5: West Coast PADD 2: Midwest PADD 1: East Coast PADD 3: Gulf Coast PADD1A: New England PADD1B: Central Atlantic PADD1C: Lower Atlantic Petroleum Administration for Defense Districts AK HI WA OR CA NV AZ MT WY CO UT ID ND SD NE KS OK MO MN WI MI IL IN OH KY TN IA NM TX AR LA AL MS WV VA NC SC GA FL ME NH VT NY PA NJ MD DE MA CT RI

  8. printer_friendly_org_chart

    Gasoline and Diesel Fuel Update (EIA)

    FL PADD 4: Rocky Mountain PADD 5: West Coast PADD 2: Midwest PADD 1: East Coast PADD 3: Gulf Coast PADD1A: New England PADD1B: Central Atlantic PADD1C: Lower Atlantic Petroleum Administration for Defense Districts AK HI WA OR CA NV AZ MT WY CO UT ID ND SD NE KS OK MO MN WI MI IL IN OH KY TN IA NM TX AR LA AL MS WV VA NC SC GA FL ME NH VT NY PA NJ MD DE MA CT RI

    Administrator U.S. Energy Information Administration Adam Sieminski Deputy Administrator Howard Gruenspecht Assistant Administrator

  9. paper-LSPP16

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FL PADD 4: Rocky Mountain PADD 5: West Coast PADD 2: Midwest PADD 1: East Coast PADD 3: Gulf Coast PADD1A: New England PADD1B: Central Atlantic PADD1C: Lower Atlantic Petroleum Administration for Defense Districts AK HI WA OR CA NV AZ MT WY CO UT ID ND SD NE KS OK MO MN WI MI IL IN OH KY TN IA NM TX AR LA AL MS WV VA NC SC GA FL ME NH VT NY PA NJ MD DE MA CT RI

    pantex On Womens Equality Day, we celebrate NNSA's talented Women in STEM NNSA's systems administrators keep the computers running

  10. Department of Energy (DOE) OpenNet documents

    Office of Scientific and Technical Information (OSTI)

    Account Request *First Name: *Last Name: *Organization: *Phone: *Email: Fax: *Address: *City: *State: Select AL AK AZ AR CA CO CT DE DC FL GA HI ID IL IN IA KS KY LA ME MD MA MI MN MS MO MT NE NV NH NJ NM NY NC ND OH OK OR PA RI SC SD TN TX UT VT VA WA WV WI WY Zip Code: *Affiliation: Select Federal Contractor *Access Level: Select View Release Official Releasing Official: release *Site Input Code: Select A2EDAP - Atmosphere to Electrons (A2e) Data Archive and Portal, Pacific Northwest National

  11. DOE Hydrogen and Fuel Cell Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE Hydrogen & Fuel Cell Overview Dr. Sunita Satyapal Program Manager U.S. Department of Energy Fuel Cell Technologies Program DOE/CESA/TTC Hydrogen and Fuel Cells Webinar December 14, 2010 2 Examples of DOE-funded Partners and Locations - Fuel Cell Technologies Program TX NM AZ NC AR CA CO HI WA IL KY MA MN MO MS AL NV TN UT WV ID FL MI ND OR OH IN MT WY IO NE KS OK AK LA GA WI SC VA PA DE MD DC NJ NY RI CT VT NH ME SD Source: US DOE 12/2010 2 3 Fuel Cells: Addressing Energy Challenges 4

  12. Print

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    < 5k 0 < 50k < 100k < 250k < 500k < 1M > 1M > 5M > 10M DE MD DC MA RI NJ AZ UT WY ID OR WA CA TX OK KS CO NE SD ND MN WI IL IA MO AR LA MS AL FL GA TN KY IN OH MI ME NH CT VT NY PA WV VA NC SC MT AK HI NV NM Princeton Plasma Physics Laboratory Procured Materials and Services 2015 (> $35M) Small business procurements in US: $14.73M

  13. Buildings Energy Data Book: 3.9 Educational Facilities

    Buildings Energy Data Book [EERE]

    6 2010 Regional New Construction and Renovations Expenditures for Public K-12 Schools ($Million) Region New Schools Additions Renovation Total Region 1 (CT, MA, ME, NH, RI, VT) Region 2 (NJ, NY, PA) Region 3 (DE, MD, VA, WV) Region 4 (KY, NC, SC, TN) Region 5 (AL, FL, GA, MS) Region 6 (IN, MI, OH) Region 7 (IL, MN, WI) Region 8 (IA, KS, MO, NE) Region 9 (AR, LA, OK, TX) Region 10 (CO, MT, ND, NM, SD, UT, WY) Region 11 (AZ, CA, HI, NV) Region 12 (AK, ID, OR, WA) Total Source(s): School Planning

  14. DOE - Office of Legacy Management -- Dow-Detroit Edison Project - MI 0-02

    Office of Legacy Management (LM)

    Dow-Detroit Edison Project - MI 0-02 FUSRAP Considered Sites Site: Dow-Detroit Edison Project (MI.0-02 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Detroit , Michigan MI.0-02-1 Evaluation Year: 1987 MI.0-02-1 Site Operations: Performed reference design work for a special fast breeder type reactor. MI.0-02-1 Site Disposition: Eliminated - No radioactive material handled at the site MI.0-02-1 Radioactive Materials Handled: No

  15. DOE - Office of Legacy Management -- Mitts-Merrill Co - MI 14

    Office of Legacy Management (LM)

    Mitts-Merrill Co - MI 14 FUSRAP Considered Sites Site: MITTS and MERRILL CO. (MI.14 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Genessee Packing Co. MI.14-1 Location: Saginaw, Michigan MI.14-1 Evaluation Year: 1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces on a small test scale during the mid-1950s. MI.14-1 Site Disposition: Eliminated - Potential for contamination considered remote based on limited quantity

  16. miRNA-205 affects infiltration and metastasis of breast cancer

    SciTech Connect (OSTI)

    Wang, Zhouquan; Department of Tumor, SenGong Hospital of Shaanxi, Xian 710300 ; Liao, Hehe; Deng, Zhiping; Yang, Po; Du, Ning; Zhanng, Yunfeng; Ren, Hong

    2013-11-08

    Highlights: We detected expression of miR-205 in breast cancer cell lines and tissue samples. We suggest miR-205 is downregulated in human breast cancer tissues and MCF7 cells. We suggest the lower expression of miR-205 play a role in breast cancer onset. These data suggest that miR-205 directly targets HER3 in human breast cancer. -- Abstract: Background: An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy. Methods: The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. Results: miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3? untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression. Conclusion: miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.

  17. NuMI Low Energy Flux Prediction Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NuMI Low Energy Flux Prediction Release Neutrino Flux Predictions for the NuMI Beam hep-ex/1607.00704 Data Ancillary data files for this result are available on arXiv at http://arxiv.org/src/1607.00704/anc.< /li> Among the available data files are: pdf file describing format of all the available files root file of all the available fluxes python code to read and process MINERvA's flux predictions Text Files of the flux, uncertainties, and covariance matrix, with units of neutrinos/m^2/POT,

  18. Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 1 2014 1 1 1 1 2 1 1 1 1 1 2015 1 1 1 1 1 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Exports by Point of Exit Port Huron, MI LNG Exports to All Countries

  19. Ground Motion Studies at NuMI

    SciTech Connect (OSTI)

    Mayda M. Velasco; Michal Szleper

    2012-02-20

    Ground motion can cause significant deterioration in the luminosity of a linear collider. Vibration of numerous focusing magnets causes continuous misalignments, which makes the beam emittance grow. For this reason, understanding the seismic vibration of all potential LC sites is essential and related efforts in many sites are ongoing. In this document we summarize the results from the studies specific to Fermilab grounds as requested by the LC project leader at FNAL, Shekhar Mishra in FY04-FY06. The Northwestern group focused on how the ground motion effects vary with depth. Knowledge of depth dependence of the seismic activity is needed in order to decide how deep the LC tunnel should be at sites like Fermilab. The measurements were made in the NuMI tunnel, see Figure 1. We take advantage of the fact that from the beginning to the end of the tunnel there is a height difference of about 350 ft and that there are about five different types of dolomite layers. The support received allowed to pay for three months of salary of Michal Szleper. During this period he worked a 100% of his time in this project. That include one week of preparation: 2.5 months of data taking and data analysis during the full period of the project in order to guarantee that we were recording high quality data. We extended our previous work and made more systematic measurements, which included detailed studies on stability of the vibration amplitudes at different depths over long periods of time. As a consequence, a better control and more efficient averaging out of the daytime variation effects were possible, and a better study of other time dependences before the actual depth dependence was obtained. Those initial measurements were made at the surface and are summarized in Figure 2. All measurements are made with equipment that we already had (two broadband seismometers KS200 from GEOTECH and DL-24 portable data recorder). The offline data analysis took advantage of the full Fourier spectra

  20. DOE - Office of Legacy Management -- Mitts-Merrel Co - MI 14

    Office of Legacy Management (LM)

    1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces ... Primary Radioactive Materials Handled: Thorium MI.14-1 Radiological Survey(s): Yes - ...

  1. MiR-125a TNF receptor-associated factor 6 to inhibit osteoclastogenesis

    SciTech Connect (OSTI)

    Guo, Li-Juan; Liao, Lan; Yang, Li; Li, Yu; Jiang, Tie-Jian

    2014-02-15

    MicroRNAs (miRNAs) play important roles in osteoclastogenesis and bone resorption. In the present study, we found that miR-125a was dramatically down-regulated during macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) induced osteoclastogenesis of circulating CD14+ peripheral blood mononuclear cells (PBMCs). Overexpression of miR-125a in CD14+ PBMCs inhibited osteoclastogenesis, while inhibition of miR-125a promoted osteoclastogenesis. TNF receptor-associated factor 6 (TRAF6), a transduction factor for RANKL/RANK/NFATc1 signal, was confirmed to be a target of miR-125a. EMSA and ChIP assays confirmed that NFATc1 bound to the promoter of the miR-125a. Overexpression of NFATc1 inhibited miR-125a transcription, and block of NFATc1 expression attenuated RANKL-regulated miR-125a transcription. Here, we reported that miR-125a played a biological function in osteoclastogenesis through a novel TRAF6/ NFATc1/miR-125a regulatory feedback loop. It suggests that regulation of miR-125a expression may be a potential strategy for ameliorating metabolic disease. - Highlights: • MiR-125a was significantly down-regulated in osteoclastogenesis of CD14+ PBMCs. • MiR-125a inhibited osteoclast differentiation by targeting TRAF6. • NFATc1 inhibited miR-125a transciption by binding to the promoter of miR-125a. • TRAF6/NFATc1 and miR-125a form a regulatory feedback loop in osteoclastogenesis.

  2. DOE - Office of Legacy Management -- Amex Specialty Metal Corp - MI 0-01

    Office of Legacy Management (LM)

    Amex Specialty Metal Corp - MI 0-01 FUSRAP Considered Sites Site: Amex Specialty Metal Corp (MI.0-01 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Coldwater , Michigan MI.0-01-1 Evaluation Year: 1987 MI.0-01-1 Site Operations: No indication that AMEX performed work for MED or AEC activities. Originally included on FUSRAP list due to fact that AMEX purchased milling equipment from a company that had done uranium milling.

  3. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma

    SciTech Connect (OSTI)

    Xiong, Xinkui; Sun, Daoyi; Chai, Hao; Shan, Wengang; Yu, Yue; Pu, Liyong; Cheng, Feng

    2015-09-18

    The dysregulation of micro (mi)RNAs is associated with cancer development. The miRNA miR-145 is downregulated in intrahepatic cholangiocarcinoma (ICC); however, its precise role in tumor progression has not yet been elucidated. Novel (nua) kinase family (NUAK)1 functions as an oncogene in various cancers and is a putative target of miR-145 regulation. In this study, we investigated the regulation of NUAK1 by miR-145 in ICC. We found that miR-145 level was significantly decreased in ICC tissue and cell lines, which corresponded with an increase in NUAK1 expression. NUAK1 was found to be a direct target of miR-145 regulation. The overexpression of miR-145 in ICC cell lines inhibited proliferation, growth, and invasion by suppressing NUAK1 expression, which was associated with a decrease in Akt signaling and matrix metalloproteinase protein expression. Similar results were observed by inhibiting NUAK1 expression. These results demonstrate that miR-145 can prevent ICC progression by targeting NUAK1 and its downstream effectors, and can therefore be useful for clinical diagnosis and targeted therapy of ICC. - Highlights: • MiR-145 suppresses ICC proliferation and invasion abilities. • We demonstrated that miR-145 directly targets NUAK1 in ICC. • MiR-145 expression in ICC was associated with Akt signaling and MMPs expression.

  4. Neutron scattering study of spin ordering and stripe pinning in superconducting <mi>La>1.93<mi>Sr>0.07<mi>CuO>4

    SciTech Connect (OSTI)

    Jacobsen, H.; Zaliznyak, I. A.; Savici, A. T.; Winn, B. L.; Chang, S.; Hücker, M.; Gu, G. D.; Tranquada, J. M.

    2015-11-20

    The relationships among charge order, spin fluctuations, and superconductivity in underdoped cuprates remain controversial. We use neutron scattering techniques to study these phenomena in <mi>La>1.93<mi>Sr>0.07<mi>CuO>4 a superconductor with a transition temperature of Tc = 20 K. At T<< Tc, we find incommensurate spin fluctuations with a quasielastic energy spectrum and no sign of a gap within the energy range from 0.2 to 15 meV. A weak elastic magnetic component grows below ~ 10 K, consistent with results from local probes. Regarding the atomic lattice, we have discovered unexpectedly strong fluctuations of the CuO6 octahedra about Cu-O bonds, which are associated with inequivalent O sites within the CuO2 planes. Moreover, we observed a weak elastic (3 30) superlattice peak that implies a reduced lattice symmetry. The presence of inequivalent O sites rationalizes various pieces of evidence for charge stripe order in underdoped La2-xSrxCuO4. The coexistence of superconductivity with quasi-static spin-stripe order suggests the presence of intertwined orders; however, the rotation of the stripe orientation away from the Cu-O bonds might be connected with evidence for a finite gap at the nodal points of the superconducting gap function.

  5. Radiosensitizing Effects of Ectopic miR-101 on Non-Small-Cell Lung Cancer Cells Depend on the Endogenous miR-101 Level

    SciTech Connect (OSTI)

    Chen, Susie; Wang Hongyan; Ng, Wooi Loon; Curran, Walter J.; Wang Ya

    2011-12-01

    Purpose: Previously, we showed that ectopic miR-101 could sensitize human tumor cells to radiation by targeting ATM and DNA-PK catalytic subunit (DNA-PKcs) to inhibit DNA repair, as the endogenous miR-101 levels are low in tumors in general. However, the heterogeneity of human cancers may result in an exception. The purpose of this study was to test the hypothesis that a few tumor cell lines with a high level of endogenous miR-101 would prove less response to ectopic miR-101. Methods and Materials: Fourteeen non-small-cell lung cancer (NSCLC) cell lines and one immortalized non-malignant lung epithelial cell line (NL20) were used for comparing endogenous miR-101 levels by real-time reverse transcription-polymerase chain reaction. Based on the different miR-101 levels, four cell lines with different miR-101 levels were chosen for transfection with a green fluorescent protein-lentiviral plasmid encoding miR-101. The target protein levels were measured by using Western blotting. The radiosensitizing effects of ectopic miR-101 on these NSCLC cell lines were determined by a clonogenic assay and xenograft mouse model. Results: The endogenous miR-101 level was similar or lower in 13 NSCLC cell lines but was 11-fold higher in one cell line (H157) than in NL20 cells. Although ectopic miR-101 efficiently decreased the ATM and DNA-PKcs levels and increased the radiosensitization level in H1299, H1975, and A549 cells, it did not change the levels of the miR-101 targets or radiosensitivity in H157 cells. Similar results were observed in xenograft mice. Conclusions: A small number of NSCLC cell lines could have a high level of endogenous miR-101. The ectopic miR-101 was able to radiosensitize most NSCLC cells, except for the NSCLC cell lines that had a much higher endogenous miR-101 level. These results suggest that when we choose one miRNA as a therapeutic tool, the endogenous level of the miRNA in each tumor should be considered.

  6. Microsoft Word - figure_14.doc

    U.S. Energy Information Administration (EIA) Indexed Site

    42 Figure 14. Net interstate movements, imports, and exports of natural gas in the United States, 2014 (million cubic feet) Norway Trinidad/ Tobago Yemen Trinidad/ Tobago Interstate Movements Not Shown on Map From Volume To From Volume To CT RI Other TX IN MA RI MA MA CT VA DC MD DC VT MA NH MA WA M T I D O R W Y ND SD C A N V U T CO NE KS A Z NM OK TX MN WI MI IA I L IN OH MO AR M S AL GA T N KY FL SC NC WV MD DE VA PA NJ NY CT RI MA VT NH ME LA HI A K Mexico C a n a d a C a n a d a Canada

  7. DLEU2, frequently deleted in malignancy, functions as a critical host gene of the cell cycle inhibitory microRNAs miR-15a and miR-16-1

    SciTech Connect (OSTI)

    Lerner, Mikael; Harada, Masako; Loven, Jakob; Castro, Juan; Davis, Zadie; Oscier, David; Henriksson, Marie; Sangfelt, Olle; Grander, Dan; Corcoran, Martin M.

    2009-10-15

    The microRNAs miR-15a and miR-16-1 are downregulated in multiple tumor types and are frequently deleted in chronic lymphocytic leukemia (CLL), myeloma and mantle cell lymphoma. Despite their abundance in most cells the transcriptional regulation of miR-15a/16-1 remains unclear. Here we demonstrate that the putative tumor suppressor DLEU2 acts as a host gene of these microRNAs. Mature miR-15a/miR-16-1 are produced in a Drosha-dependent process from DLEU2 and binding of the Myc oncoprotein to two alterative DLEU2 promoters represses both the host gene transcript and levels of mature miR-15a/miR-16-1. In line with a functional role for DLEU2 in the expression of the microRNAs, the miR-15a/miR-16-1 locus is retained in four CLL cases that delete both promoters of this gene and expression analysis indicates that this leads to functional loss of mature miR-15a/16-1. We additionally show that DLEU2 negatively regulates the G1 Cyclins E1 and D1 through miR-15a/miR-16-1 and provide evidence that these oncoproteins are subject to miR-15a/miR-16-1-mediated repression under normal conditions. We also demonstrate that DLEU2 overexpression blocks cellular proliferation and inhibits the colony-forming ability of tumor cell lines in a miR-15a/miR-16-1-dependent way. Together the data illuminate how inactivation of DLEU2 promotes cell proliferation and tumor progression through functional loss of miR-15a/miR-16-1.

  8. Genome-Wide Analysis of miRNA targets in Brachypodium and Biomass Energy Crops

    SciTech Connect (OSTI)

    Green, Pamela J.

    2015-08-11

    MicroRNAs (miRNAs) contribute to the control of numerous biological processes through the regulation of specific target mRNAs. Although the identities of these targets are essential to elucidate miRNA function, the targets are much more difficult to identify than the small RNAs themselves. Before this work, we pioneered the genome-wide identification of the targets of Arabidopsis miRNAs using an approach called PARE (German et al., Nature Biotech. 2008; Nature Protocols, 2009). Under this project, we applied PARE to Brachypodium distachyon (Brachypodium), a model plant in the Poaceae family, which includes the major food grain and bioenergy crops. Through in-depth global analysis and examination of specific examples, this research greatly expanded our knowledge of miRNAs and target RNAs of Brachypodium. New regulation in response to environmental stress or tissue type was found, and many new miRNAs were discovered. More than 260 targets of new and known miRNAs with PARE sequences at the precise sites of miRNA-guided cleavage were identified and characterized. Combining PARE data with the small RNA data also identified the miRNAs responsible for initiating approximately 500 phased loci, including one of the novel miRNAs. PARE analysis also revealed that differentially expressed miRNAs in the same family guide specific target RNA cleavage in a correspondingly tissue-preferential manner. The project included generation of small RNA and PARE resources for bioenergy crops, to facilitate ongoing discovery of conserved miRNA-target RNA regulation. By associating specific miRNA-target RNA pairs with known physiological functions, the research provides insights about gene regulation in different tissues and in response to environmental stress. This, and release of new PARE and small RNA data sets should contribute basic knowledge to enhance breeding and may suggest new strategies for improvement of biomass energy crops.

  9. miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A

    SciTech Connect (OSTI)

    Shao, Mingchen; Geng, Yiwei; Lu, Peng; Xi, Ying; Wei, Sidong; Wang, Liuxing; Fan, Qingxia; Ma, Wang

    2015-09-04

    MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention. - Highlights: • miR-4295 mimics promote the proliferation and invasion of ATC cells. • miR-4295 inhibitors inhibit the proliferation and invasion of ATC cells. • miR-4295 targets 3′UTR of CDKN1A in ATC cells. • miR-4295 negatively regulates CDKN1A in ATC cells.

  10. Microfluidic Molecular Assay Platform for the Detection of miRNAs...

    Office of Scientific and Technical Information (OSTI)

    Article: Microfluidic Molecular Assay Platform for the Detection of miRNAs, mRNAs, Proteins, and Post-translational Modifications at Single-cell Resolution. Citation Details...

  11. Groundwater protection for the NuMI project

    SciTech Connect (OSTI)

    Wehmann, A.; Smart, W.; Menary, S.; Hylen, J.; Childress, S.

    1997-10-01

    The physics requirements for the long base line neutrino oscillation experiment MINOS dictate that the NuMI beamline be located in the aquifer at Fermilab. A methodology is described for calculating the level of radioactivation of groundwater caused by operation of this beamline. A conceptual shielding design for the 750 meter long decay pipe is investigated which would reduce radioactivation of the groundwater to below government standards. More economical shielding designs to meet these requirements are being explored. Also, information on local geology, hydrogeology, government standards, and a glossary have been included.

  12. Highgate Springs, VT LNG Imports from Canada

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Data Series Area 2009 2010 2011 2012 2013...

  13. Highgate Springs, VT LNG Imports from Canada

    Gasoline and Diesel Fuel Update (EIA)

    Cubic Feet) Dollars per Thousand Cubic Feet) Hidalgo, TX Natural Gas Pipeline Imports From Mexico (Dollars per Thousand Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 2.26 2.31 2.03 2.09 2000's 5.85 4.61 2.26 -- -- 8.10 5.53 6.23 5.55 4.40 2010's 4.21 -- -- -- -- - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016

  14. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma

    SciTech Connect (OSTI)

    Zhai, Jian; Qu, Shuping; Li, Xiaowei; Zhong, Jiaming; Chen, Xiaoxia; Qu, Zengqiang; Wu, Dong

    2015-08-14

    Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights: • Decreased of miR-129 is found in HCC and associated with advanced stage and metastasis. • miR-129 suppresses proliferation and invasion of HCC cells. • miR-129 directly targets the 3′ UTR of PAK5 and diminishes PAK5 expression. • PAK5 is involved in miR-129 mediated suppression functions.

  15. MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19

    SciTech Connect (OSTI)

    Shan, Nianxi; Shen, Liangfang; Wang, Jun; He, Dan; Duan, Chaojun

    2015-01-02

    Highlights: • Decreased miR-153 and up-regulated ADAM19 are correlated with NSCLC pathology. • MiR-153 inhibits the proliferation and migration and invasion of NSCLC cells in vitro. • ADAM19 is a direct target of miR-153. • ADAM19 is involved in miR-153-suppressed migration and invasion of NSCLC cells. - Abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3′UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which was similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.

  16. Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells

    SciTech Connect (OSTI)

    Li, Junxia; Shan, Fabo; Xiong, Gang; Wang, Ju-Ming; Wang, Wen-Lin; Xu, Xueqing; Bai, Yun

    2014-03-28

    Highlights: • MiR-146b promotes esophageal cancer cell proliferation. • MiR-146b inhibits esophageal cancer cell apoptosis. • C/EBPβ directly binds to miR-146b promoter conserved region. • MiR-146b is up-regulated by C/EBPβ LAP2 transcriptional activation. - Abstract: Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.

  17. Oil-shale utilization at Morgantown, WV

    SciTech Connect (OSTI)

    Shang, J.Y.; Notestein, J.E.; Mei, J.S.; Romanosky, R.R.; King, J.A.; Zeng, L.W.

    1982-01-01

    Fully aware of the nation's need to develop high-risk and long-term research in eastern oil-shale and low-grade oil-shale utilization in general, the US DOE/METC initiated an eastern oil-shale characterization program. In less than 3 months, METC produced shale oil from a selected eastern-US oil shale with a Fischer assay of 8.0 gallons/ton. In view of the relatively low oil yield from this particular oil shale, efforts were directed to determine the process conditions which give the highest oil yield. A 2-inch-diameter electrically heated fluidized-bed retort was constructed, and Celina oil shale from Tennessee was selected to be used as a representative eastern oil shale. After more than 50 runs, the retorting data were analyzed and reviewed and the best oil-yield operating condition was determined. In addition, while conducting the oil-shale retorting experiments, a number of technical problems were identified, addressed, and overcome. Owing to the inherent high rates of heat and mass transfers inside the fluidized bed, the fluidized-bed combustor and retorting appear to be a desirable process technology for an effective and efficient means for oil-shale utilization. The fluidized-bed operation is a time-tested, process-proven, high-throughput, solid-processing operation which may contribute to the efficient utilization of oil-shale energy.

  18. miR-30a suppresses breast cancer cell proliferation and migration by targeting Eya2

    SciTech Connect (OSTI)

    Fu, Jing; Xu, Xiaojie; Kang, Lei; Zhou, Liying; Wang, Shibin; Lu, Juming; Cheng, Long; Fan, Zhongyi; Yuan, Bin; Tian, Peirong; Zheng, Xiaofei; Yu, Chengze; Ye, Qinong; Lv, Zhaohui

    2014-03-07

    Highlights: • miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. • The miR-30a/EYA2 axis regulates breast cancer cell proliferation and migration. • The miR-30a/EYA2 axis modulates G1/S cell cycle progression. • The miR-30a/EYA2 axis is dysregulated in breast cancer patients. - Abstract: Eye absent (Eya) proteins are involved in cell fate determination in a broad spectrum of cells and tissues. Aberrant expression of Eya2 has been documented in a variety of cancers and correlates with clinical outcome. However, whether microRNAs (miRNAs) can regulate Eya2 expression remains unknown. Here, we show that miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. Overexpression of Eya2 in miR-30a-transfected breast cancer cells effectively rescued the inhibition of cell proliferation and migration caused by miR-30a. Knockdown of Eya2 by small-interfering RNA (siRNA) in breast cancer cells mimicked the effect induced by miR-30a and abolished the ability of miR-30a to regulate breast cancer cell proliferation and migration. The miR-30a/Eya2 axis could regulate G1/S cell cycle progression, accompanied by the modulation of expression of cell cycle-related proteins, including cyclin A, cyclin D1, cyclin E, and c-Myc. Moreover, miR-30a expression was downregulated in breast cancer patients, and negatively correlated with Eya2, which was upregulated in breast cancer patients. These data suggest that the miR-30a/Eya2 axis may play an important role in breast cancer development and progression and that miR-30a activation or Eya2 inhibition may be a useful strategy for cancer treatment.

  19. miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7

    SciTech Connect (OSTI)

    Zhou, Chuanyi; Shen, Liangfang; Mao, Lei; Wang, Bing; Li, Yang; Yu, Huizhi

    2015-02-27

    MicroRNAs (miRNAs) are involved in the cervical carcinogenesis and progression. In this study, we investigated the role of miR-92a in progression and invasion of cervical cancer. MiR-92a was significantly upregulated in cervical cancer tissues and cell lines. Overexpression of miR-92a led to remarkably enhanced proliferation by promoting cell cycle transition from G1 to S phase and significantly enhanced invasion of cervical cancer cells, while its knockdown significantly reversed these cellular events. Bioinformatics analysis suggested F-box and WD repeat domain-containing 7 (FBXW7) as a novel target of miR-92a, and miR-92a suppressed the expression level of FBXW7 mRNA by direct binding to its 3′-untranslated region (3′UTR). Expression of miR-92a was negatively correlated with FBXW7 in cervical cancer tissues. Furthermore, Silencing of FBXW7 counteracted the effects of miR-92a suppression, while its overexpression reversed oncogenic effects of miR-92a. Together, these findings indicate that miR-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, suggesting miR-92a as a potential novel diagnostic and therapeutic target of cervical cancer. - Highlights: • miR-92a is elevated in cervical cancer tissues and cell lines. • miR-92a promotes cervical cancer cell proliferation, cell cycle transition from G1 to S phase and invasion. • FBXW7 is a direct target of miR-92a. • FBXW7 counteracts the oncogenic effects of miR-92a on cervical cancer cells.

  20. miR-182 targets CHL1 and controls tumor growth and invasion in papillary thyroid carcinoma

    SciTech Connect (OSTI)

    Zhu, Hongling; Fang, Jin; Zhang, Jichen; Zhao, Zefei; Liu, Lianyong; Wang, Jingnan; Xi, Qian; Gu, Mingjun

    2014-07-18

    Highlights: miR-182 and CHL1 expression patterns are negatively correlated. CHL1 is a direct target of miR-182 in PTC cells. miR-182 suppression inhibits PTC cell growth and invasion. CHL1 is involved in miR-182-mediated cell behavior. - Abstract: In this study, we investigated the role and underlying mechanism of action of miR-182 in papillary thyroid carcinoma (PTC). Bioinformatics analysis revealed close homolog of LI (CHL1) as a potential target of miR-182. Upregulation of miR-182 was significantly correlated with CHL1 downregulation in human PTC tissues and cell lines. miR-182 suppressed the expression of CHL1 mRNA through direct targeting of the 3?-untranslated region (3?-UTR). Downregulation of miR-182 suppressed growth and invasion of PTC cells. Silencing of CHL1 counteracted the effects of miR-182 suppression, while its overexpression mimicked these effects. Our data collectively indicate that miR-182 in PTC promotes cell proliferation and invasion through direct suppression of CHL1, supporting the potential utility of miR-182 inhibition as a novel therapeutic strategy against PTC.

  1. Non-canonical microRNAs miR-320 and miR-702 promote proliferation in Dgcr8-deficient embryonic stem cells

    SciTech Connect (OSTI)

    Kim, Byeong-Moo; Choi, Michael Y.

    2012-09-21

    Highlights: Black-Right-Pointing-Pointer Embryonic stem cells (ESCs) lacking non-canonical miRNAs proliferate slower. Black-Right-Pointing-Pointer miR-320 and miR-702 are two non-canonical miRNAs expressed in ESCs. Black-Right-Pointing-Pointer miR-320 and miR-702 promote proliferation of Dgcr8-deficient ESCs. Black-Right-Pointing-Pointer miR-320 targets p57 and helps to release Dgcr8-deficient ESCs from G1 arrest. Black-Right-Pointing-Pointer miR-702 targets p21 and helps to release Dgcr8-deficient ESCs from G1 arrest. -- Abstract: MicroRNAs are known to contribute significantly to stem cell phenotype by post-transcriptionally regulating gene expression. Most of our knowledge of microRNAs comes from the study of canonical microRNAs that require two sequential cleavages by the Drosha/Dgcr8 heterodimer and Dicer to generate mature products. In contrast, non-canonical microRNAs bypass the cleavage by the Drosha/Dgcr8 heterodimer within the nucleus but still require cytoplasmic cleavage by Dicer. The function of non-canonical microRNAs in embryonic stem cells (ESCs) remains obscure. It has been hypothesized that non-canonical microRNAs have important roles in ESCs based upon the phenotypes of ESC lines that lack these specific classes of microRNAs; Dicer-deficient ESCs lacking both canonical and non-canonical microRNAs have much more severe proliferation defect than Dgcr8-deficient ESCs lacking only canonical microRNAs. Using these cell lines, we identified two non-canonical microRNAs, miR-320 and miR-702, that promote proliferation of Dgcr8-deficient ESCs by releasing them from G1 arrest. This is accomplished by targeting the 3 Prime -untranslated regions of the cell cycle inhibitors p57 and p21 and thereby inhibiting their expression. This is the first report of the crucial role of non-canonical microRNAs in ESCs.

  2. NuMI proton kicker extraction magnet termination resistor system

    SciTech Connect (OSTI)

    Reeves, S.R.; Jensen, C.C.; /Fermilab

    2005-05-01

    The temperature stability of the kicker magnet termination resistor assembly directly affects the field flatness and amplitude stability. Comprehensive thermal enhancements were made to the existing Main Injector resistor assembly design to satisfy NuMI performance specifications. Additionally, a fluid-processing system utilizing Fluorinert{reg_sign} FC-77 high-voltage dielectric was built to precisely control the setpoint temperature of the resistor assembly from 70 to 120F, required to maintain constant resistance during changing operational modes. The Fluorinert{reg_sign} must be continually processed to remove hazardous breakdown products caused by radiation exposure to prevent chemical attack of system components. Design details of the termination resistor assembly and Fluorinert{reg_sign} processing system are described. Early performance results will be presented.

  3. Downregulation of miRNA-30c and miR-203a is associated with hepatitis C virus core protein-induced epithelial–mesenchymal transition in normal hepatocytes and hepatocellular carcinoma cells

    SciTech Connect (OSTI)

    Liu, Dongjing; Wu, Jilin; Liu, Meizhou; Yin, Hui; He, Jiantai; Zhang, Bo

    2015-09-04

    Hepatitis C virus (HCV) Core protein has been demonstrated to induce epithelial–mesenchymal transition (EMT) and is associated with cancer progression of hepatocellular carcinoma (HCC). However, how the Core protein regulates EMT is still unclear. In this study, HCV Core protein was overexpressed by an adenovirus. The protein levels of EMT markers were measured by Western blot. The xenograft animal model was established by inoculation of HepG2 cells. Results showed that ectopic expression of HCV core protein induced EMT in L02 hepatocytes and HepG2 tumor cells by upregulating vimentin, Sanl1, and Snal2 expression and downregulating E-cadherin expression. Moreover, Core protein downregulated miR-30c and miR-203a levels in L02 and HepG2 cells, but artificial expression of miR-30c and miR-203a reversed Core protein-induced EMT. Further analysis showed that ectopic expression of HCV core protein stimulated cell proliferation, inhibited apoptosis, and increased cell migration, whereas artificial expression of miR-30c and miR-203a significantly reversed the role of Core protein in these cell functions in L02 and HepG2 cells. In the HepG2 xenograft tumor models, artificial expression of miR-30c and miR-203a inhibited EMT and tumor growth. Moreover, L02 cells overexpressing Core protein can form tumors in nude mice. In HCC patients, HCV infection significantly shortened patients' survival time, and loss of miR-30c and miR-203 expression correlated with poor survival. In conclusion, HCV core protein downregulates miR-30c and miR-203a expression, which results in activation of EMT in normal hepatocytes and HCC tumor cells. The Core protein-activated-EMT is involved in the carcinogenesis and progression of HCC. Loss of miR-30c and miR-203a expression is a marker for the poor prognosis of HCC. - Highlights: • HCV core protein downregulates miR-30c and miR-203a expression. • Downregulation of miR-30c and miR-203a activates EMT. • Activated-EMT is involved in the

  4. miR-128 and its target genes in tumorigenesis and metastasis

    SciTech Connect (OSTI)

    Li, Molin, E-mail: molin_li@hotmail.com [Dalian Medical University, Dalian 116044 (China); Fu, Weiming [Center for Food Safety and Environmental Technology, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458 (China); Wo, Lulu; Shu, Xiaohong [Dalian Medical University, Dalian 116044 (China); Liu, Fang [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China); Li, Chuangang, E-mail: li_chuangang@sina.com [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China)

    2013-12-10

    MicroRNAs (miRNAs) are a class of endogenous, non-coding, 1824 nucleotide length single-strand RNAs that could modulate gene expression at post-transcriptional level. Previous studies have shown that miR-128 enriched in the brain plays an important role in the development of nervous system and the maintenance of normal physical functions. Aberrant expression of miR-128 has been detected in many types of human tumors and its validated target genes are involved in cancer-related biological processes such as cell proliferation, differentiation and apoptosis. In this review, we will summarize the roles of miR-128 and its target genes in tumorigenesis and metastasis. - Highlights: Aberrant expression of miR-128 can be observed in many kinds of malignant tumors. The molecular mechanisms regulating miR-128 expression are elucidated. Roles of miR-128 and its target genes in tumorigenesis and metastasis are summarized.

  5. miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

    SciTech Connect (OSTI)

    Mizuno, Yosuke; Yagi, Ken; Tokuzawa, Yoshimi; Kanesaki-Yatsuka, Yukiko; Suda, Tatsuo; Katagiri, Takenobu; Fukuda, Toru; Maruyama, Masayoshi; Okuda, Akihiko; Amemiya, Tomoyuki; Kondoh, Yasumitsu; Tashiro, Hideo; Okazaki, Yasushi

    2008-04-04

    Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

  6. Overexpression of miR-206 suppresses glycolysis, proliferation and migration in breast cancer cells via PFKFB3 targeting

    SciTech Connect (OSTI)

    Ge, Xin; Lyu, Pengwei; Cao, Zhang; Li, Jingruo; Guo, Guangcheng; Xia, Wanjun; Gu, Yuanting

    2015-08-07

    miRNAs, sorting as non-coding RNAs, are differentially expressed in breast tumor and act as tumor promoters or suppressors. miR-206 could suppress the progression of breast cancer, the mechanism of which remains unclear. The study here was aimed to investigate the effect of miR-206 on human breast cancers. We found that miR-206 was down-regulated while one of its predicted targets, 6-Phosphofructo-2-kinase (PFKFB3) was up-regulated in human breast carcinomas. 17β-estradiol dose-dependently decreased miR-206 expression as well as enhanced PFKFB3 mRNA and protein expression in estrogen receptor α (ERα) positive breast cancer cells. Furthermore, we identified that miR-206 directly interacted with 3′-untranslated region (UTR) of PFKFB3 mRNA. miR-206 modulated PFKFB3 expression in MCF-7, T47D and SUM159 cells, which was influenced by 17β-estradiol depending on ERα expression. In addition, miR-206 overexpression impeded fructose-2,6-bisphosphate (F2,6BP) production, diminished lactate generation and reduced cell proliferation and migration in breast cancer cells. In conclusion, our study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration. - Highlights: • miR-206 was down-regulated and PFKFB3 was up-regulated in human breast carcinomas. • 17β-estradiol regulated miR-206 and PFKFB3 expression in ERα+ cancer cells. • miR-206directly interacted with 3′-UTR of PFKFB3 mRNA. • miR-206 fructose-2,6-bisphosphate (F2,6BP) impeded production and lactate generation. • miR-206 reduced cell proliferation and migration in breast cancer cells.

  7. AMENDMENT OF SOLICITATION/MODIFICATlON OF CONTRACT MI54 I See...

    National Nuclear Security Administration (NNSA)

    MI54 I See Block 16C I REQ. NO. Babcock & Wilcox Technical Services Pantex, LLC PO Box 30020 Amarillo, TX 79120 2. AMENDMENTIMODIFICATION NO. 1 3. EFFECTIVE DATE 1 4. ...

  8. File:USDA-CE-Production-GIFmaps-MI.pdf | Open Energy Information

    Open Energy Info (EERE)

    MI.pdf Jump to: navigation, search File File history File usage Michigan Ethanol Plant Locations Size of this preview: 463 599 pixels. Other resolution: 464 600 pixels. Full...

  9. Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Sault Ste. Marie Tribe of Chippewa Indians, MI Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI The Sault Ste. Marie Tribe of Chippewa Indians is a 44,000-strong federally recognized Indian tribe that is an economic, social and cultural force in its community across the eastern Upper Peninsula counties of Chippewa, Luce, Mackinac, Schoolcraft, Alger, Delta and Marquette, with housing and tribal centers, casinos, and other enterprises that employ

  10. miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells

    SciTech Connect (OSTI)

    Zhang, Jie; Zheng, Fangxia; Yu, Gang; Yin, Yanhua; Lu, Qingyang

    2013-11-01

    Highlights: miR-196a was overexpressed in cervical cancer tissue compared to normal tissue. miR-196a expression elevated proliferation and migration of cervical cancer cells. miR-196a inhibited NTN4 expression by binding 3?-UTR region of NTN4 mRNA. NTN4 inversely correlated with miR-196a expression in cervical tissue and cell line. NTN4 expression was low in cervical cancer tissue compared to normal tissue. -- Abstract: Recent research has uncovered tumor-suppressive and oncogenic potential of miR-196a in various tumors. However, the expression and mechanism of its function in cervical cancer remains unclear. In this study, we assess relative expression of miR-196a in cervical premalignant lesions, cervical cancer tissues, and four cancer cell lines using quantitative real-time PCR. CaSki and HeLa cells were treated with miR-196a inhibitors, mimics, or pCDNA/miR-196a to investigate the role of miR-196a in cancer cell proliferation and migration. We demonstrated that miR-196a was overexpressed in cervical intraepithelial neoplasia 23 and cervical cancer tissue. Moreover, its expression contributes to the proliferation and migration of cervical cancer cells, whereas inhibiting its expression led to a reduction in proliferation and migration. Five candidate targets of miR-196a chosen by computational prediction and Cervical Cancer Gene Database search were measured for their mRNA in both miR-196a-overexpressing and -depleted cancer cells. Only netrin 4 (NTN4) expression displayed an inverse association with miR-196a. Fluorescent reporter assays revealed that miR-196a inhibited NTN4 expression by targeting one binding site in the 3?-untranslated region (3?-UTR) of NTN4 mRNA. Furthermore, qPCR and Western blot assays verified NTN4 expression was downregulated in cervical cancer tissues compared to normal controls, and in vivo mRNA level of NTN4 inversely correlated with miR-196a expression. In summary, our findings provide new insights about the functional role of

  11. miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1

    SciTech Connect (OSTI)

    Xu, Zhengyu; Wang, Tao

    2014-06-27

    Highlights: • miR-214 is upregulated in human OS tissues and inversely correlated with LZTS1 expression. • miR-214 directly targets LZTS1 by binding to its 3′-UTR. • miR-214 promotes OS cell proliferation, invasion and tumor growth. • Overexpression of LZTS1 reverses miR-214-induced proliferation and invasion of OS cells. - Abstract: Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3′-untranslated region (3′-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.

  12. Microsoft Word - figure_03.doc

    U.S. Energy Information Administration (EIA) Indexed Site

    Oil and Gas Reserves"; PointLogic Energy; Ventyx; and the Bureau of Safety and Environmental Enforcement, and predecessor agencies. IN OH TN WV VA KY MD PA NY VT NH MA CT ME RI ...

  13. miR-421 induces cell proliferation and apoptosis resistance in human nasopharyngeal carcinoma via downregulation of FOXO4

    SciTech Connect (OSTI)

    Chen, Liang; Department of Otolaryngology, Guangzhou General Hospital of PLA Guangzhou Command, Guangzhou 510010 ; Tang, Yanping; Wang, Jian; Yan, Zhongjie; Xu, Ruxiang

    2013-06-14

    Highlights: •miR-421 is upregulated in nasopharyngeal carcinoma. •miR-421 induces cell proliferation and apoptosis resistance. •FOXO4 is a direct and functional target of miR-421. -- Abstract: microRNAs have been demonstrated to play important roles in cancer development and progression. Hence, identifying functional microRNAs and better understanding of the underlying molecular mechanisms would provide new clues for the development of targeted cancer therapies. Herein, we reported that a microRNA, miR-421 played an oncogenic role in nasopharyngeal carcinoma. Upregulation of miR-421 induced, whereas inhibition of miR-421 repressed cell proliferation and apoptosis resistance. Furthermore, we found that upregulation of miR-421 inhibited forkhead box protein O4 (FOXO4) signaling pathway following downregulation of p21, p27, Bim and FASL expression by directly targeting FOXO4 3′UTR. Additionally, we demonstrated that FOXO4 expression is critical for miR-421-induced cell growth and apoptosis resistance. Taken together, our findings not only suggest that miR-421 promotes nasopharyngeal carcinoma cell proliferation and anti-apoptosis, but also uncover a novel regulatory mechanism for inactivation of FOXO4 in nasopharyngeal carcinoma.

  14. Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

    SciTech Connect (OSTI)

    Zhou, Yuan; Wang, Hui; Wang, Cong; Qiu, Xuefeng; Benson, Mikael; Yin, Xiaoqin; Xiang, Zou; Li, Dongmei; and others

    2015-08-15

    Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system. - Highlights: • miRNAs were altered in Sertoli cells exposed to MC-LR. • Alerted genes were involved in different cell functions including the cell morphology. • MC-LR adversely affected Sertoli cell junction formation through the regulating miRNAs.

  15. Ionizing Radiation–Inducible miR-27b Suppresses Leukemia Proliferation via Targeting Cyclin A2

    SciTech Connect (OSTI)

    Wang, Bo; Li, Dongping; Kovalchuk, Anna; Litvinov, Dmitry; Kovalchuk, Olga

    2014-09-01

    Purpose: Ionizing radiation is a common carcinogen that is important for the development of leukemia. However, the underlying epigenetic mechanisms remain largely unknown. The goal of the study was to explore microRNAome alterations induced by ionizing radiation (IR) in murine thymus, and to determine the role of IR-inducible microRNA (miRNA/miR) in the development of leukemia. Methods and Materials: We used the well-established C57BL/6 mouse model and miRNA microarray profiling to identify miRNAs that are differentially expressed in murine thymus in response to irradiation. TIB152 human leukemia cell line was used to determine the role of estrogen receptor–α (ERα) in miR-27b transcription. The biological effects of ectopic miR-27b on leukemogenesis were measured by western immunoblotting, cell viability, apoptosis, and cell cycle analyses. Results: Here, we have shown that IR triggers the differential expression of miR-27b in murine thymus tissue in a dose-, time- and sex-dependent manner. miR-27b was significantly down-regulated in leukemia cell lines CCL119 and TIB152. Interestingly, ERα was overexpressed in those 2 cell lines, and it was inversely correlated with miR-27b expression. Therefore, we used TIB152 as a model system to determine the role of ERα in miR-27b expression and the contribution of miR-27b to leukemogenesis. β-Estradiol caused a rapid and transient reduction in miR-27b expression reversed by either ERα-neutralizing antibody or ERK1/2 inhibitor. Ectopic expression of miR-27b remarkably suppressed TIB152 cell proliferation, at least in part, by inducing S-phase arrest. In addition, it attenuated the expression of cyclin A2, although it had no effect on the levels of PCNA, PPARγ, CDK2, p21, p27, p-p53, and cleaved caspase-3. Conclusion: Our data reveal that β-estradiol/ERα signaling may contribute to the down-regulation of miR-27b in acute leukemia cell lines through the ERK1/2 pathway, and that miR-27b may function as a tumor

  16. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    SciTech Connect (OSTI)

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  17. Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression

    SciTech Connect (OSTI)

    Xiao, Can; Wang, Lili; Zhu, Lifang; Zhang, Chenping; Zhou, Jianhua

    2014-11-28

    Highlights: • miR-9 expression level was significantly decreased in OSCC tissues. • Curcumin significantly inhibited SCC-9 cells proliferation. • miR-9 mediates the inhibition of SCC-9 proliferation by curcumin. • Curcumin suppresses Wnt/β-catenin signaling in SCC-9 cells. • miR-9 mediates the suppression of Wnt/β-catenin signaling by curcumin. - Abstract: Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells, but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future.

  18. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  19. Word Pro - Untitled1

    U.S. Energy Information Administration (EIA) Indexed Site

    State-Level Energy Consumption Estimates and Estimated Consumption per Capita, 2010 Consumption Consumption per Capita 14 U.S. Energy Information Administration / Annual Energy Review 2011 TX CA FL LA IL OH PA NY GA IN MI NC VA NJ TN WA KY AL MO MN WI SC OK CO IA MD AZ MA MS KS AR OR NE UT CT WV NM NV AK WY ID ND ME MT SD NH HI DE RI DC VT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 0 2 4 6 8 10

  20. Port Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    to Canada (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 1 2014 1 1 1 1 2 1 1 1 1 1 2015 1 1 1 1 1 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Exports by Point of Exit Port Huron, MI Natural Gas Exports to Canad

  1. Post Mortem of 120k mi Light-Duty Urea SCR and DPF System | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Post Mortem of 120k mi Light-Duty Urea SCR and DPF System Post Mortem of 120k mi Light-Duty Urea SCR and DPF System Presentation given at the 2007 Diesel Engine-Efficiency & Emissions Research Conference (DEER 2007). 13-16 August, 2007, Detroit, Michigan. Sponsored by the U.S. Department of Energy's (DOE) Office of FreedomCAR and Vehicle Technologies (OFCVT). deer07_lambert.pdf (649.68 KB) More Documents & Publications Urea SCR and DPF System for Tier 2 Diesel Light-Duty

  2. MicroRNAs expression in ox-LDL treated HUVECs: MiR-365 modulates apoptosis and Bcl-2 expression

    SciTech Connect (OSTI)

    Qin, Bing; Xiao, Bo; Liang, Desheng; Xia, Jian; Li, Ye; Yang, Huan

    2011-06-24

    Highlights: {yields} We evaluated the role of miRNAs in ox-LDL induced apoptosis in ECs. {yields} We found 4 up-regulated and 11 down-regulated miRNAs in apoptotic ECs. {yields} Target genes of the dysregulated miRNAs regulate ECs apoptosis and atherosclerosis. {yields} MiR-365 promotes ECs apoptosis via suppressing Bcl-2 expression. {yields} MiR-365 inhibitor alleviates ECs apoptosis induced by ox-LDL. -- Abstract: Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play a critical role in atherosclerosis. MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. However, whether miRNAs are associated with ox-LDL induced apoptosis and their effect on ECs is still unknown. Therefore, this study evaluated potential miRNAs and their involvement in ECs apoptosis in response to ox-LDL stimulation. Microarray and qRT-PCR analysis performed on human umbilical vein endothelial cells (HUVECs) exposed to ox-LDL identified 15 differentially expressed (4 up- and 11 down-regulated) miRNAs. Web-based query tools were utilized to predict the target genes of the differentially expressed miRNAs, and the potential target genes were classified into different function categories with the gene ontology (GO) term and KEGG pathway annotation. In particular, bioinformatics analysis suggested that anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl-2) is a target gene of miR-365, an apoptomir up-regulated by ox-LDL stimulation in HUVECs. We further showed that transfection of miR-365 inhibitor partly restored Bcl-2 expression at both mRNA and protein levels, leading to a reduction of ox-LDL-mediated apoptosis in HUVECs. Taken together, our findings indicate that miRNAs participate in ox-LDL-mediated apoptosis in HUVECs. MiR-365 potentiates ox-LDL-induced ECs apoptosis by regulating the

  3. MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ

    SciTech Connect (OSTI)

    Yuan, Jian; Xiao, Gelei; Peng, Gang; Liu, Dingyang; Wang, Zeyou; Liao, Yiwei; Liu, Qing; Wu, Minghua; Yuan, Xianrui

    2015-02-06

    Highlights: • Expression of miR-125a-5p is inversely correlated with that of TAZ in glioma cells. • MiR-125a-5p represses TAZ expression in glioma cells. • MiR-125a-5p directly targets the 3′ UTR of TAZ mRNA and promotes its degradation. • MiR-125a-5p represses CTGF and survivin via TAZ, and inhibits glioma cell growth. • MiR-125a-5p inhibits the stem cell features of HFU-251 MG cells. - Abstract: Glioblastoma (GBM) is the most lethal brain tumor due to the resistance to conventional therapies, such as radiotherapy and chemotherapy. TAZ, an important mediator of the Hippo pathway, was found to be up-regulated in diverse cancers, including in GBM, and plays important roles in tumor initiation and progression. However, little is known about the regulation of TAZ expression in tumors. In this study, we found that miR-125a-5p is an important regulator of TAZ in glioma cells by directly targeting the TAZ 3′ UTR. MiR-125a-5p levels are inversely correlated with that of TAZ in normal astrocytes and a panel of glioma cell lines. MiR-125a-5p represses the expression of TAZ target genes, including CTGF and survivin, and inhibits cell proliferation and induces the differentiation of GBM cells; whereas over-expression of TAZ rescues the effects of miR-125a-5p. This study revealed a mechanism for TAZ deregulation in glioma cells, and also demonstrated a tumor suppressor role of miR-125a-5p in glioblastoma cells.

  4. The NuMI proton beam at Fermilab successes and challenges

    SciTech Connect (OSTI)

    Childress, S.; /Fermilab

    2008-11-01

    The NuMI beam at Fermilab has delivered over 5 x 10{sup 20} 120 GeV protons to the neutrino production target since the start for MINOS [1] neutrino oscillation experiment operation in 2005. We report on proton beam commissioning and operation status, including successes and challenges with this beam.

  5. DOE Zero Energy Ready Home Case Study: Cobblestone Homes, Midland, MI

    Broader source: Energy.gov [DOE]

    Case study of a DOE Zero Energy Ready home in Midland, MI, that scored HERS 49 without PV or HERS 44 with 1.4 kW of PV. The custom home served as a prototype and energy efficiency demonstration...

  6. MiR-18a regulates the proliferation, migration and invasion of human glioblastoma cell by targeting neogenin

    SciTech Connect (OSTI)

    Song, Yichen; Wang, Ping; Zhao, Wei; Yao, Yilong; Liu, Xiaobai; Ma, Jun; Xue, Yixue; Liu, Yunhui

    2014-05-15

    MiR-17-92 cluster has recently been reported as an oncogene in some tumors. However, the association of miR-18a, an important member of this cluster, with glioblastoma remains unknown. Therefore, this study aims to investigate the expression of miR-18a in glioblastoma and its role in biological behavior of U87 and U251 human glioblastoma cell lines. Quantitative RT-PCR results showed that miR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines compared with that in human brain tissues and primary normal human astrocytes, and the expression levels were increased along with the rising pathological grades of glioblastoma. Neogenin was identified as the target gene of miR-18a by dual-luciferase reporter assays. RT-PCR and western blot results showed that its expression levels were decreased along with the rising pathological grades of glioblastoma. Inhibition of miR-18a expression was established by transfecting exogenous miR-18a inhibitor into U87 and U251 cells, and its effects on the biological behavior of glioblastoma cells were studied using CCK-8 assay, transwell assay and flow cytometry. Inhibition of miR-18a expression in U87 and U251 cells significantly up-regulated neogenin, and dramatically suppressed the abilities of cell proliferation, migration and invasion, induced cell cycle arrest and promoted cellular apoptosis. Collectively, these results suggest that miR-18a may regulate biological behavior of human glioblastoma cells by targeting neogenin, and miR-18a can serve as a potential target in the treatment of glioblastoma. - Highlights: • MiR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines. • Neogenin was identified as the target gene of miR-18a. • Neogenin expressions were decreased along with the rising pathological grades of glioblastoma. • Inhibition of miR-18a suppressed biological behavior of glioma cells by up-regulating neogenin.

  7. miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer

    SciTech Connect (OSTI)

    Li, Qiaoyan; Zhu, Fufan; Chen, Puxiang

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Both miR-7 and miR-218 down-regulates HoxB3 expression by targeting the 3 Prime -UTR of HoxB3 mRNA. Black-Right-Pointing-Pointer A reverse correlation between the levels of endogenous miR-7, miR218 and HoxB3 expression. Black-Right-Pointing-Pointer Epigenetic changes involve in the reactivation of HoxB3. Black-Right-Pointing-Pointer Both miRNAs inhibits the cell cycle and clone formation of breast cancer cells. -- Abstract: Many microRNAs have been implicated as key regulators of cellular growth and differentiation and have been found to dysregulate proliferation in human tumors, including breast cancer. Cancer-linked microRNAs also alter the epigenetic landscape by way of DNA methylation and post-translational modifications of histones. Aberrations in Hox gene expression are important for oncogene or tumor suppressor during abnormal development and malignancy. Although recent studies suggest that HoxB3 is critical in breast cancer, the putative role(s) of microRNAs impinging on HoxB3 is not yet fully understood. In this study, we found that the expression levels of miR-7 and miR-218 were strongly and reversely associated with HoxB3 expression. Stable overexpression of miR-7 and miR-218 was accompanied by reactivation of tumor suppressor genes including RASSF1A and Claudin-6 by means of epigenetic switches in DNA methylation and histone modification, giving rise to inhibition of the cell cycle and clone formation of breast cancer cells. The current study provides a novel link between overexpression of collinear Hox genes and multiple microRNAs in human breast malignancy.

  8. Loss of expression of miR-335 is implicated in hepatic stellate cell migration and activation

    SciTech Connect (OSTI)

    Chen, Chao; Wu, Chao-Qun; Zhang, Zong-Qi; Yao, Ding-Kang; Zhu, Liang

    2011-07-15

    Activation and migration of resident stellate cells (HSCs) within the hepatic space of Disse play an important role in hepatic fibrosis, which accounts for the increased numbers of activated HSCs in areas of inflammation during hepatic fibrosis. Currently, microRNAs have been found to play essential roles in HSC differentiation, proliferation, apoptosis, fat accumulation and collagen production. However, little is known about microRNA mediated HSC activation and migration. In this study, the miRNA expression profiles of quiescent HSCs, partially activated HSCs and fully activated HSCs were compared in pairs. Gene ontology (GO) and GO-Map network analysis indicated that the activation of HSCs was regulated by microRNAs. Among them miR-335 was confirmed to be significantly reduced during HSC activation by qRT-PCR, and restoring expression of miR-335 inhibited HSC migration and reduced {alpha}-SMA and collagen type I. Previous study revealed that tenascin-C (TNC), an extracellular matrix glycoprotein involved in cell migration, might be a target of miR-335. Therefore, we further studied the TNC expression in miR-335 over-expressed HSCs. Our data showed that exogenous TNC could enhance HSC migration in vitro and miR-335 restoration resulted in a significant inhibition of TNC expression. These results demonstrated that miR-335 restoration inhibited HSC migration, at least in part, via downregulating the TNC expression.

  9. PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

    SciTech Connect (OSTI)

    Zheng, Peihao; Guo, Honggang; Li, Guangchao; Han, Siqi; Luo, Fei; Liu, Yi

    2015-03-06

    Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.

  10. Aryl hydrocarbon receptor-dependent regulation of miR-196a expression controls lung fibroblast apoptosis but not proliferation

    SciTech Connect (OSTI)

    Hecht, Emelia; Zago, Michela; Sarill, Miles; Rico de Souza, Angela; Gomez, Alvin; Matthews, Jason; Hamid, Qutayba; Eidelman, David H.; Baglole, Carolyn J.

    2014-11-01

    The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor implicated in the regulation of apoptosis and proliferation. Although activation of the AhR by xenobiotics such as dioxin inhibits the cell cycle and control apoptosis, paradoxically, AhR expression also promotes cell proliferation and survival independent of exogenous ligands. The microRNA (miRNA) miR-196a has also emerged as a regulator of proliferation and apoptosis but a relationship between the AhR and miR-196a is not known. Therefore, we hypothesized that AhR-dependent regulation of endogenous miR-196a expression would promote cell survival and proliferation. Utilizing lung fibroblasts from AhR deficient (AhR{sup −/−}) and wild-type (AhR{sup +/+}) mice, we show that there is ligand-independent regulation of miRNA, including low miR-196a in AhR{sup −/−} cells. Validation by qRT-PCR revealed a significant decrease in basal expression of miR-196a in AhR{sup −/−} compared to AhR{sup +/+} cells. Exposure to AhR agonists benzo[a]pyrene (B[a]P) and FICZ as well as AhR antagonist CH-223191 decreased miR-196a expression in AhR{sup +/+} fibroblasts concomitant with decreased AhR protein levels. There was increased proliferation only in AhR{sup +/+} lung fibroblasts in response to serum, corresponding to a decrease in p27{sup KIP1} protein, a cyclin-dependent kinase inhibitor. Increasing the cellular levels of miR-196a had no effect on proliferation or expression of p27{sup KIP1} in AhR{sup −/−} fibroblasts but attenuated cigarette smoke-induced apoptosis. This study provides the first evidence that AhR expression is essential for the physiological regulation of cellular miRNA levels- including miR-196a. Future experiments designed to elucidate the functional relationship between the AhR and miR-196a may delineate additional novel ligand-independent roles for the AhR. - Highlights: • The AhR controls proliferation and apoptosis in lung cells. • The AhR regulates the

  11. RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR...

    Office of Scientific and Technical Information (OSTI)

    in prostate cancer Citation Details In-Document Search Title: RLIP76-dependent suppression of PI3KAKTBcl-2 pathway by miR-101 induces apoptosis in prostate cancer MicroRNA-101 ...

  12. Material Activation Benchmark Experiments at the NuMI Hadron Absorber Hall in Fermilab

    SciTech Connect (OSTI)

    Matsumura, H.; Matsuda, N.; Kasugai, Y.; Toyoda, A.; Yashima, H.; Sekimoto, S.; Iwase, H.; Oishi, K.; Sakamoto, Y.; Nakashima, H.; Leveling, A.; Boehnlein, D.; Lauten, G.; Mokhov, N.; Vaziri, K.

    2014-06-15

    In our previous study, double and mirror symmetric activation peaks found for Al and Au arranged spatially on the back of the Hadron absorber of the NuMI beamline in Fermilab were considerably higher than those expected purely from muon-induced reactions. From material activation bench-mark experiments, we conclude that this activation is due to hadrons with energy greater than 3 GeV that had passed downstream through small gaps in the hadron absorber.

  13. Port Huron, MI Natural Gas Pipeline Imports From Canada (Million Cubic

    U.S. Energy Information Administration (EIA) Indexed Site

    Feet) Million Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 262 278 16 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S.

  14. Targeting miR-21 enhances the sensitivity of human colon cancer HT-29 cells to chemoradiotherapy in vitro

    SciTech Connect (OSTI)

    Deng, Jun; Lei, Wan; Fu, Jian-Chun; Zhang, Ling; Li, Jun-He; Xiong, Jian-Ping

    2014-01-17

    Highlight: MiR-21 plays a significant role in 5-FU resistance. This role might be attributed to targeting of hMSH2 as well as TP and DPD via miR-21 targeted hMSH2. Indirectly targeted TP and DPD to influence 5-FU chemotherapy sensitivity. -- Abstract: 5-Fluorouracil (5-FU) is a classic chemotherapeutic drug that has been widely used for colorectal cancer treatment, but colorectal cancer cells are often resistant to primary or acquired 5-FU therapy. Several studies have shown that miR-21 is significantly elevated in colorectal cancer. This suggests that this miRNA might play a role in this resistance. In this study, we investigated this possibility and the possible mechanism underlying this role. We showed that forced expression of miR-21 significantly inhibited apoptosis, enhanced cell proliferation, invasion, and colony formation ability, promoted G1/S cell cycle transition and increased the resistance of tumor cells to 5-FU and X radiation in HT-29 colon cancer cells. Furthermore, knockdown of miR-21 reversed these effects on HT-29 cells and increased the sensitivity of HT-29/5-FU to 5-FU chemotherapy. Finally, we showed that miR-21 targeted the human mutS homolog2 (hMSH2), and indirectly regulated the expression of thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD). These results demonstrate that miR-21 may play an important role in the 5-FU resistance of colon cancer cells.

  15. miR-21 modulates tumor outgrowth induced by human adipose tissue-derived mesenchymal stem cells in vivo

    SciTech Connect (OSTI)

    Shin, Keun Koo; Lee, Ae Lim; Kim, Jee Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870; BK21 Medical Science Education Center, School of Medicine, Pusan National University, Yangsan, Gyeongnam 626-870 ; Lee, Sun Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870 ; Bae, Yong Chan; Jung, Jin Sup

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer miR-21 modulates hADSC-induced increase of tumor growth. Black-Right-Pointing-Pointer The action is mostly mediated by the modulation of TGF-{beta} signaling. Black-Right-Pointing-Pointer Inhibition of miR-21 enhances the blood flow recovery in hindlimb ischemia. -- Abstract: Mesenchymal stem cells (MSCs) have generated a great deal of interest in clinical situations, due principally to their potential use in regenerative medicine and tissue engineering applications. However, the therapeutic application of MSCs remains limited, unless the favorable effects of MSCs on tumor growth in vivo, and the long-term safety of the clinical applications of MSCs, can be more thoroughly understood. In this study, we determined whether microRNAs can modulate MSC-induced tumor outgrowth in BALB/c nude mice. Overexpression of miR-21 in human adipose-derived stem cells (hADSCs) inhibited hADSC-induced tumor growth, and inhibition of miR-21 increased it. Downregulation of transforming growth factor beta receptor II (TGFBR2), but not of signal transducer and activator of transcription 3, in hADSCs showed effects similar to those of miR-21 overexpression. Downregulation of TGFBR2 and overexpression of miR21 decreased tumor vascularity. Inhibition of miR-21 and the addition of TGF-{beta} increased the levels of vascular endothelial growth factor and interleukin-6 in hADSCs. Transplantation of miR-21 inhibitor-transfected hADSCs increased blood flow recovery in a hind limb ischemia model of nude mice, compared with transplantation of control oligo-transfected cells. These findings indicate that MSCs might favor tumor growth in vivo. Thus, it is necessary to study the long-term safety of this technique before MSCs can be used as therapeutic tools in regenerative medicine and tissue engineering.

  16. Measurement of Pi-K Ratios from the NuMI Target

    SciTech Connect (OSTI)

    Seun, Sin Man; /Harvard U.

    2007-07-01

    Interactions of protons (p) with the NuMI (Neutrinos at the Main Injector) target are used to create the neutrino beam for the MINOS (Main Injector Neutrino Oscillation Search) Experiment. Using the MIPP (Main Injector Particle Production) experimental apparatus, the production of charged pions and kaons in p+NuMI interactions is studied. The data come from a sample of 2 x 10{sup 6} events obtained by MIPP using the 120 GeV/c proton beam from the Main Injector at Fermi National Accelerator Laboratory in Illinois, USA. Pions and kaons are identified by measurement in a Ring Imaging Cherenkov detector. Presented are measurements of {pi}{sup -}/{pi}{sup +}, K{sup -}/K{sup +}, {pi}{sup +}/K{sup +} and {pi}{sup -}/K{sup -} production ratios in the momentum range p{sub T} < 2 GeV/c transversely and 20 GeV/c < p{sub z} < 90 GeV/c longitudinally. Also provided are detailed comparisons of the MIPP NuMI data with the MIPP Thin Carbon data, the MIPP Monte Carlo simulation and the current MINOS models in the relevant momentum ranges.

  17. miR-502 inhibits cell proliferation and tumor growth in hepatocellular carcinoma through suppressing phosphoinositide 3-kinase catalytic subunit gamma

    SciTech Connect (OSTI)

    Chen, Suling; Li, Fang; Chai, Haiyun; Tao, Xin; Wang, Haili; Ji, Aifang

    2015-08-21

    MicroRNAs (miRNAs) play a key role in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). In the present study, we demonstrated that miR-502 significantly inhibits HCC cell proliferation in vitro and tumor growth in vivo. G1/S cell cycle arrest and apoptosis of HCC cells were induced by miR-502. Phosphoinositide 3-kinase catalytic subunit gamma (PIK3CG) was identified as a direct downstream target of miR-502 in HCC cells. Notably, overexpression of PIK3CG reversed the inhibitory effects of miR-502 in HCC cells. Our findings suggest that miR-502 functions as a tumor suppressor in HCC via inhibition of PI3KCG, supporting its utility as a promising therapeutic gene target for this tumor type. - Highlights: • miR-502 suppresses HCC cell proliferation in vitro and tumorigenicity in vivo. • miR-502 regulates cell cycle and apoptosis in HCC cells. • PIK3CG is a direct target of miR-502. • miR-502 and PIK3CG expression patterns are inversely correlated in HCC tissues.

  18. MiR-145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1

    SciTech Connect (OSTI)

    Wu, Huijuan; Xiao, ZhengHua; Wang, Ke; Liu, Wenxin; Hao, Quan

    2013-11-29

    Highlights: MiR-145 is downregulated in human ovarian cancer. MiR-145 targets p70S6K1 and MUC1. p70S6K1 and MUC1 are involved in miR-145 mediated tumor cell growth and cell invasion, respectively. -- Abstract: MicroRNAs (miRNAs) are a family of small non-coding RNA molecules that regulate gene expression at post-transcriptional levels. Previous studies have shown that miR-145 is downregulated in human ovarian cancer; however, the roles of miR-145 in ovarian cancer growth and invasion have not been fully demonstrated. In the present study, Northern blot and qRT-PCR analysis indicate that miR-145 is downregulated in ovarian cancer tissues and cell lines, as well as in serum samples of ovarian cancer, compared to healthy ovarian tissues, cell lines and serum samples. Functional studies suggest that miR-145 overexpression leads to the inhibition of colony formation, cell proliferation, cell growth viability and invasion, and the induction of cell apoptosis. In accordance with the effect of miR-145 on cell growth, miR-145 suppresses tumor growth in vivo. MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3?UTRs. Importantly, the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145, respectively. MiR-145 expression is increased after 5-aza-CdR treatment, and 5-aza-CdR treatment results in the same phenotype as the effect of miR-145 overexpression. Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1, functioning as a tumor suppressor. Moreover, our data imply that miR-145 has potential as a miRNA-based therapeutic target for ovarian cancer.

  19. Testing CPT conservation using the NuMI neutrino beam with the MINOS experiment

    SciTech Connect (OSTI)

    Auty, David John

    2010-05-01

    The MINOS experiment was designed to measure neutrino oscillation parameters with muon neutrinos. It achieves this by measuring the neutrino energy spectrum and flavor composition of the man-made NuMI neutrino beam 1km after the beam is formed and again after 735 km. By comparing the two spectra it is possible to measure the oscillation parameters. The NuMI beam is made up of 7.0% {bar {nu}}{sub {mu}}, which can be separated from the {nu}{sub {mu}} because the MINOS detectors are magnetized. This makes it possible to study {bar {nu}}{sub {mu}} oscillations separately from those of muon neutrinos, and thereby test CPT invariance in the neutrino sector by determining the {bar {nu}}{sub {mu}} oscillation parameters and comparing them with those for {nu}{sub {mu}}, although any unknown physics of the antineutrino would appear as a difference in oscillation parameters. Such a test has not been performed with beam {bar {nu}}{sub {mu}} before. It is also possible to produce an almost pure {bar {nu}}{sub {mu}} beam by reversing the current through the magnetic focusing horns of the NuMI beamline, thereby focusing negatively, instead of positively charged particles. This thesis describes the analysis of the 7% {bar {nu}}{sub {mu}} component of the forward horn current NuMI beam. The {bar {nu}}{sub {mu}} of a data sample of 3.2 x 10{sup 20} protons on target analysis found 42 events, compared to a CPT conserving prediction of 58.3{sub -7.6}{sup +7.6}(stat.){sub -3.6}{sup +3.6}(syst.) events. This corresponds to a 1.9 {sigma} deficit, and a best fit value of {Delta}{bar m}{sub 32}{sup 2} = 18 x 10{sup -3} eV{sup 2} and sin{sup 2} 2{bar {theta}}{sub 23} = 0.55. This thesis focuses particularly on the selection of {bar {nu}}{sub {mu}} events, and investigates possible improvements of the selection algorithm. From this a different selector was chosen, which corroborated the findings of the original selector. The thesis also investigates how the systematic errors affect the

  20. Port Huron, MI Natural Gas Pipeline Imports From Canada (Dollars per

    U.S. Energy Information Administration (EIA) Indexed Site

    Thousand Cubic Feet) Dollars per Thousand Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Dollars per Thousand Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 2.07 2.06 2.21 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Price of

  1. DOE Zero Ready Home Case Study: Cobblestone Homes, 2014 Model Home, Midland, MI

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Cobblestone Homes 2014 Model Home Midland, MI DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced technologies are designed

  2. miR-340 inhibits glioblastoma cell proliferation by suppressing CDK6, cyclin-D1 and cyclin-D2

    SciTech Connect (OSTI)

    Li, Xuesong; Gong, Xuhai; Chen, Jing; Zhang, Jinghui; Sun, Jiahang; Guo, Mian

    2015-05-08

    Glioblastoma development is often associated with alteration in the activity and expression of cell cycle regulators, such as cyclin-dependent kinases (CKDs) and cyclins, resulting in aberrant cell proliferation. Recent studies have highlighted the pivotal roles of miRNAs in controlling the development and growth of glioblastoma. Here, we provide evidence for a function of miR-340 in the inhibition of glioblastoma cell proliferation. We found that miR-340 is downregulated in human glioblastoma tissue samples and several established glioblastoma cell lines. Proliferation and neurosphere formation assays revealed that miR-340 plays an oncosuppressive role in glioblastoma, and that its ectopic expression causes significant defect in glioblastoma cell growth. Further, using bioinformatics, luciferase assay and western blot, we found that miR-340 specifically targets the 3′UTRs of CDK6, cyclin-D1 and cyclin-D2, leading to the arrest of glioblastoma cells in the G0/G1 cell cycle phase. Confirming these results, we found that re-introducing CDK6, cyclin-D1 or cyclin-D2 expression partially, but significantly, rescues cells from the suppression of cell proliferation and cell cycle arrest mediated by miR-340. Collectively, our results demonstrate that miR-340 plays a tumor-suppressive role in glioblastoma and may be useful as a diagnostic biomarker and/or a therapeutic avenue for glioblastoma. - Highlights: • miR-340 is downregulated in glioblastoma samples and cell lines. • miR-340 inhibits glioblastoma cell proliferation. • miR-340 directly targets CDK6, cyclin-D1, and cyclin-D2. • miR-340 regulates glioblastoma cell proliferation via CDK6, cyclin-D1 and cyclin-D2.

  3. Assessment of radiological releases from the NuMI facility during MINOS and NOvA operations

    SciTech Connect (OSTI)

    Martens, Mike; /Fermilab

    2007-04-01

    This report makes projections of the radiological releases from the NuMI facility during operations for the MINOS and NO ?A experiments. It includes an estimate of the radionuclide levels released into the atmosphere and the estimated tritium and sodium-22 concentrations in the NuMI sump water and Fermilab pond system. The analysis was performed for NuMI operations with a beam power on target increased from the present 400 kW design up to a possible 1500 kW with future upgrades. The total number of protons on target was assumed to be 18 x 10{sup 20} after the completion of MINOS and 78 x 10{sup 20} after the completion of NO ?A.

  4. RAPID/Roadmap/7-VT-c | Open Energy Information

    Open Energy Info (EERE)

    the Board, the applicable utility, local planning commission, the municipal legislative body of the town in which the system is to be installed, the Planning Division, Agency of...

  5. North Troy, VT Natural Gas Imports by Pipeline from Canada

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Lease Separation 73 98 82 179 192 1979-2014 Adjustments -15 33 33 -2 2 25 1979-2014 Revision Increases 6 10 12 11 87 60 1979-2014 Revision Decreases 9 6 5 13 8 65 1979-2014 Sales 2 6 51 1 0 2 2000-2014 Acquisitions 0 0 51 0 31 12 2000-2014 Extensions 0 0 0 2 1 1 1979-2014 New Field Discoveries 0 0 0 0 0 0 1979-2014 New Reservoir Discoveries in Old Fields 2 1 0 0 0 0 1979-2014 Estimated Production 7 12 15 13 16 18 Production

    8 0 0 0 0 0 2009-2014 Acquisitions 0 0 0 0 0 0 2009-2014

  6. RAPID/Roadmap/6-VT-b | Open Energy Information

    Open Energy Info (EERE)

    a total land disturbance of equal to or greater than one acre, where those discharges enter waters of Vermont or a conveyance leading to waters of Vermont. 10 V.S.A. ...

  7. Microsoft Word - Allco Comments and Motion to Intervene VT Green...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... OCS under the No Action Alternative, consumers could obtain oil and gas from other ... For example, BOEM detailed how "Coal consumed in place of gas under the No Action ...

  8. RAPID/Roadmap/18-VT-b | Open Energy Information

    Open Energy Info (EERE)

    and Rebuttable presumption for used oil. Used oil containing more than 1000 ppm total halogens is presumed to be a hazardous waste because it has been mixed with halogenated...

  9. RAPID/Roadmap/3-VT-b | Open Energy Information

    Open Energy Info (EERE)

    controlled by the Department of Forests, Parks and Recreation (FPR), the Department of Fish and Wildlife (DFW), and the Department of Environmental Conservation (DEC). These lands...

  10. Highgate Springs, VT Natural Gas Pipeline Imports From Canada (Million

    U.S. Energy Information Administration (EIA) Indexed Site

    Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 7,711 8,136 7,680 8,141 2000's 9,980 7,815 8,421 8,272 8,761 8,392 8,404 8,021 8,106 9,319 2010's 8,895 10,319 8,247 9,769 10,557 12,445

  11. Highgate Springs, VT Natural Gas Pipeline Imports From Canada (Million

    U.S. Energy Information Administration (EIA) Indexed Site

    Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2011 1,477 1,317 1,172 1,059 690 503 462 433 520 894 706 1,086 2012 1,322 1,116 858 681 409 309 319 323 357 509 914 1,130 2013 1,385 1,223 1,070 752 457 386 401 426 467 663 1,078 1,461 2014 1,588 1,335 1,441 872 574 431 433 435 476 616 995 1,360 2015 1,882 1,615 1,501 874 501 575 581 547 572 1,056 1,299 1,441 2016 1,866 1,651 1,493 1,440 1,156 577

  12. Climate Action Champions: Montpelier, VT | Department of Energy

    Office of Environmental Management (EM)

    - including electricity, heat and transportation - by the year 2030. The city has demonstrated its leadership and innovation in climate mitigation and resilience by creating a ...

  13. Microsoft Word - FY 16 VT Program-Wide Selection Table

    Energy Savers [EERE]

    Ex Parte Memo.docx Microsoft Word - Ex Parte Memo.docx Microsoft Word - Ex Parte Memo.docx (25.34 KB) More Documents & Publications Ex Parte Communication, July 18, 2012 Meeting Ex Parte Memorandum - Natural Resources Defense Council Ex Parte Communication Memorandum

    8 Department of Energy No. 2003-03R Financial Assistance Regulation Date 10/28/03 FINANCIAL ASSISTANCE LETTER Financial Assistance Letter is issued under the authority of the Procurement Executives of DOE and NNSA Subject:

  14. North Troy, VT Natural Gas Imports by Pipeline from Canada

    Gasoline and Diesel Fuel Update (EIA)

    17,273 26,136 27,411 18,467 17,112 19,837 1979-2014 Natural Gas Nonassociated, Wet After Lease Separation 17,220 26,063 27,313 18,385 16,933 19,645 1979-2014 Natural Gas Associated-Dissolved, Wet After Lease Separation 53 73 98 82 179 192 1979-2014 Dry Natural Gas 17,143 26,030 27,337 18,418 17,044 19,722 Separation

    17,220 26,063 27,313 18,385 16,933 19,645 1979-2014 Adjustments 154 -484 144 124 224 177 1979-2014 Revision Increases 1,168 2,594 3,093 2,913 2,527 2,378 1979-2014 Revision

  15. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    samples. With scan times lasting only seconds, the system can capture, in real time, the migration of fluids and changes in rock material at in-situ petroleum and CO 2 storage ...

  16. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Successful implementation of portfolio research objectives will ultimately increase America's domestic oil and gas supply, reduce our nation's dependency on foreign imports, and ...

  17. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of recoverable petroleum within a reservoir, as well as the modeling of the flow of these fluids within the porous media and in wellbore. These properties are also used to design...

  18. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Conversion Model allows for the transfer of elements from the JetPlume and Transport models, taking care to best amalgamate the two contrasting approaches in each, while...

  19. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Geomechanical Impacts of Shale Gas Activities Background Hydraulic fracturing of gas shale is the injection of large volumes of fluid at high pressures in low permeability shale to ...

  20. EIS-0361: Western Greenbrier Co-Production Demonstration Project, WV

    Broader source: Energy.gov [DOE]

    This EIS is about the potential environmental impacts of the U.S. Department of Energy’s (DOE’s) proposal to provide federal financial assistance for the construction and demonstration of a 98 megawatt (MWe) net power plant and cement manufacturing facility to be located in the municipality of Rainelle, Greenbrier County, West Virginia.

  1. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    which can be then used in an inexpensive "breathalyzer" to test for and monitor diabetes. The NETLSC has also greatly accelerated progress on the development of...

  2. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The facility was originally used to study the fate of CO 2 in the deep ocean, released ... Goals and Objectives The goal of the current research is to obtain fundamental, ...

  3. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Oil and natural gas fuel America's economy and account for more than 60 percent of the energy ... The results can also be used to identify requirements for new materials with superior ...

  4. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technology Transfer at NETL Carbon capture, quantum mechanical simulations, integrated gasification, and clean power-words like these mean the future of energy to NETL's in-house...

  5. Argus Energy WV, LLC wins 2007 Wetlands West Virginia Award

    SciTech Connect (OSTI)

    2007-07-15

    Argus Energy's Kiah Creek Operation has received the 2007 Wetlands West Virginia Award presented by the West Virginian Coal Association. The operation was originally a 1267 acre underground mine in the Coalburg seam. Underground mining commenced in 2000 until the end of 2003 with more than two million tons of coal being produced. The creation of the wetlands was achieved during the operations. 8 photos.

  6. Microsoft PowerPoint - WV SGIP 101810 rev1.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Smart Grid is "transactive" and will: * Enable active participation by consumers * ... electricity grid and * Address the role of coal in Smart Grid * Support economic ...

  7. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    lower heat capacity, and reduced heat of reaction. The result is a lower overall cost for CO 2 capture and separation. Many different types of solid materials have been...

  8. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    kelly.rose@netl.doe.gov Jennifer Bauer Geospatial Researcher 541-918-4507 ... These interpretations support geospatial and geo-statistical evaluations associated with ...

  9. Albany, OR * Archorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Jennifer Bauer Geospatial Researcher 541-918-4507 jennifer.bauer@contr.netl.doe.gov ... The original databook was started by NETL's Geology and Geospatial (G&G) team for the U.S. ...

  10. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    kelly.rose@netl.doe.gov Jennifer Bauer Geospatial Researcher 541-918-4507 ... The original databook was started by NETL's Geology and Geospatial (G&G) team for the U.S. ...

  11. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and model data on high performance computers with pre-loaded software, such as ArcGIS, Petra, EarthVision, GoldSim, MATLAB, and other advanced analytical, statistical and...

  12. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    their datasets using top-of-the-line research computers with key software, such as ArcGIS, Petra, GoldSim, and Earthvision, among other advanced geostatistical and analytical...

  13. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    quantifiable and relevant para- meters, while leaving the sample available for further testing. Facilities Medical CT Scanner Core-scale Characterization and Fluid Flow The...

  14. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Inc. Equitable Resources Exxon Mobile Florida Municipal ... to monitor injection operations and to ensure public safety. ... to collect data on the performance of the lower part of the ...

  15. Albany, OR * Archorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cynthia Powell Director 541-967-5803 cynthia.powell@netl.doe.gov Grant Bromhal Technical Portfolio Lead National Risk Assessment Program 304-285-4688 grant.bromhal@netl.doe.gov ...

  16. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    John Baltrus Acting Division Director Molecular Science Division 412-386-4570 john.baltrus@netl.doe.gov Paul Turner Division Director Materials Characterization Division...

  17. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Carbon capture, quantum mechanical simulations, integrated gasification, and clean ... And, as technology transfer cutting-edge inventions to present a wide energy research ...

  18. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Structural Materials are being developed for use in extreme environments associated with combustion, turbine, gasification, drilling, and other applications. Research focuses on ...

  19. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the exploration and production of deepwater and ultra-deepwater resources. Adequate definition of materials performance and properties is critical to this effort. The outcome...

  20. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Chemistry and Surface Science CONTACTS OFFICE OF RESEARCH AND DEVELOPMENT Madhava Syamlal ... Computational Chemistry Research in Support of Future Energy Technologies Background ...

  1. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    activities to test and evaluate technologies; validate and couple geomechanical and flow reservoir models to provide accurate and reliable simulations in fractured reservoirs...

  2. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    * Life prediction based on environmental and ...recipitation-strengthened nickel superalloys for oil and gas ... for these alloys as a function of borehole conditions. ...

  3. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    are an important target for studies seeking to positively affect both the efficiency and environmental impact of U.S. energy production. The diversity of available sources for...

  4. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Advanced Combustion Project addresses fundamental issues of fire-side and steam-side corrosion in oxy-fuel combustion environments. NETL's advanced ultra-supercritical (A-USC)...

  5. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and ultimately CO 2 capture cost. The NETL-ORD is also conducting system and economic studies to R& D FAC T S Carbon Capture OFFICE OF RESEARCH AND DEVELOPMENT David Alman...

  6. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Optimal Model Complexity in Geological Carbon Sequestration: A Response Surface Uncertainty Analysis Background The goal of the Department of Energy's (DOE) Carbon Storage Program...

  7. West Virginia Smart Grid Implementation Plan (WV SGIP) Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    West Virginia Natural Gas Gross Withdrawals (Million Cubic Feet) West Virginia Natural Gas Gross Withdrawals (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 17,794 15,880 17,289 16,401 16,256 15,660 15,973 15,968 15,638 17,037 16,994 17,715 1992 16,181 14,439 15,437 14,931 15,051 14,580 14,882 14,715 14,553 15,620 15,473 16,137 1993 15,164 14,540 14,678 13,676 13,828 13,984 13,701 14,144 14,031 14,506 13,568 15,205 1994 16,294 15,623 15,772 14,695 14,859 15,027

  8. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fossil Energy Plants estimated that the use of MEA to capture 90% of CO 2 in a pulverized coal power plant would impose a 30% energy penalty and ultimately result in an 85%...

  9. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computational Science and Engineering 304-285-4685 madhava.syamlal@netl.doe.gov David Miller Technical Director Carbon Capture Simulation Initiative 412-386-6555...

  10. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    are cheap and easy to process but are limited by an inherent tradeoff between permeability and selectivity - polymeric membranes can have high permeability or high...

  11. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Enhanced Analytical Simulation Tool for CO2 Storage Capacity Estimation and Uncertainty Quantification Background The goal of the Department of Energy's (DOE) Carbon Storage...

  12. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and minimal soot formation. The syngas reformate will be used as fuel for solid oxide fuel cells developed in the Solid State Energy Conversion Alliance (SECA) program....

  13. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of State Model Development for Extreme Temperatures and Pressures Background The density and viscosity of natural gas and crude oil at reservoir conditions are critical...

  14. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Turbine Thermal Management The gas turbine is the workhorse of power generation, and technology advances to current land-based turbines are directly linked to our country's...

  15. Albany, OR * Archorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    require the production of clean hydrogen to fuel innovative combustion turbines and fuel cells. This research will focus on development and assessment of membranes tailored...

  16. West Virginia Smart Grid Implementation Plan (WV SGIP) Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... For instance, computers would detect transmission bottlenecks and direct power around them. Power outages are now monitored as customers call local utilities to report them. Smart ...

  17. West Virginia Smart Grid Implementation Plan (WV SGIP) Project

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in Table 1 and the graphs shown in Figure 2 that the total of customer reported problems for voltage quality and power outages have approximately doubled in the past ten years. ...

  18. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 emissions from power plants that use fossil fuels ... first be overcome by methods that do not ... for repowering existing coal-fired facilities to capture CO ...

  19. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    gas turbine is the workhorse of power generation, and technology advances to current land-based turbines are directly linked to our country's economic and energy security....

  20. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and a burner) is coupled to heat exchangers and a turbine in order to evaluate the dynamics of a fully integrated system. R& D FAC T S Energy Systems Dynamics OFFICE OF...

  1. Albany, OR * Fairbanks, AK * Morgantown, WV * Pittsburgh, PA * Houston, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NETL R&D Tackles Technological Challenges of the Williston Basin's Bakken Formation Recent development of the Bakken Formation in the Williston Basin of western North Dakota and eastern Montana is a good example of persistent analysis of geologic data and adaptation of new completion technologies overcoming the challenges posed by unconventional reservoirs. However, as with most unconventional plays, as Bakken development continues, questions regarding exactly how to refine newly applied

  2. Albany, OR * Fairbanks, AK * Morgantown, WV * Pittsburgh, PA * Sugarland, TX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sugarland, TX Website: www.netl.doe.gov Customer Service: 1-800-553-7681 Enhanced Oil Recovery Program The mission of the Enhanced Oil Recovery Program is to provide information and technologies that will assure sustainable, reliable, affordable, and environmentally sound supplies of domestic oil resources. The Strategic Center for Natural Gas and Oil (SCNGO) seeks to accomplish this critical mission by advancing environmentally responsible technological solutions that enhance recovery of oil

  3. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    or moved into other parts of the capture portfolio for further development. Among the materials currently being examined are advanced polymers based on inorganic phosphazines and...

  4. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Deleterious Events Associated with Drilling and Production Background Increasingly, ... prediction of potential deleterious events in extreme offshore drilling and production. ...

  5. Microsoft PowerPoint - WV SGIP 101810 rev1.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Virginia Smart Grid Implementation Plan - Roadmap Framework GridWeek 2010 Steve Pullins October 18, 2010, Washington, DC This material is based upon work supported by the Department of Energy under Award Number DE AC26 04NT41817 Number DE-AC26-04NT41817 This presentation was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States g y Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or

  6. Albany, OR * Archorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... This recognition sparked a desire within NETL to leverage rapidly evolving technology, capabilities, and approaches to information sharing, big data, and computational resources, ...

  7. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... The goal is to simulate the conditions found in all of the major potential geological ... Objectives * Obtain representative strata samples per programproject constraints, working ...

  8. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... and differential scanning calori- metry, NETL researchers test geological and environmental material samples to determine degradation and decom- position temperatures, absorbed ...

  9. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of efficient and economical approaches to carbon capture. A typical coal gasification process produces H 2 , CO 2 , and steam at about 260 C and 25 bar after...

  10. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in High Pressure, High Temperature (HPHT) Ultra-Deep Drilling Environments Background Oil and natural gas fuel America's economy-accounting for more than 60 percent of the...

  11. SBOT WEST VIRGINIA NATIONAL ENERGY TECHNOLOGY LAB -WV POC Larry...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... 928120 REAL ESTATE & EQUIPMENT LEASING RENTAL Lessors of Residential Buildings and Dwellings 531110 Lessors of Nonresidential Buildings (except Miniwarehouses) 531120 Lessors of ...

  12. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... (a GC-FID with sample introduction via thermal desorption) to measure VOC's; * Air Pollution Instruments gaseous monitors for NO x and O 3 ; * A Davis Instruments ...

  13. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... via thermal desorption) to measure volatile organic compounds (VOCs); * Air Pollution Instruments gaseous monitors for NO x and O 3 ; * R.M. Young and Davis Instruments ...

  14. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to focus on only the most promising materials. Substances designed using fundamental approaches are synthesized and characterized in NETL-ORD's fully equipped synthetic...

  15. Albany, OR * Anchorage, AK * Morgantown, WV * Pittsburgh, PA...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    methods, limited variability is available in the final cathode structures. New approaches focus on generation of advanced microstructures that are more conducive to...

  16. MiR-138 promotes smooth muscle cells proliferation and migration in db/db mice through down-regulation of SIRT1

    SciTech Connect (OSTI)

    Xu, Juan; Li, Li; Yun, Hui-fang; Han, Ye-shan

    2015-08-07

    Background: Diabetic vascular smooth muscle cells (VSMCs) exhibit significantly increased rates of proliferation and migration, which was the most common pathological change in atherosclerosis. In addition, the study about the role for miRNAs in the regulation of VSMC proliferation is just beginning to emerge and additional miRNAs involved in VSMC proliferation modulation should be identified. Methods: The expression of miR-138 and SIRT1 were examined in SMCs separated from db/db mice and in SMC lines C-12511 exposed to high glucose with qRT-PCR and western blot. The regulation of miR-138 on the expression of SMCs was detected with luciferase report assay. VSMCs proliferation and migration assays were performed to examine the effect of miR-138 inhibitor on VSMCs proliferation and migration. Results: We discovered that higher mRNA level of miR-138 and reduced expression of SIRT1 were observed in SMCs separated from db/db mice and in SMC lines C-12511. Moreover, luciferase report assay showed that the activity of SIRT1 3′-UTR was highly increased by miR-138 inhibitor and reduced by miR-138 mimic. In addition, we examined that the up-regulation of NF-κB induced by high glucose in SMCs was reversed by resveratrol and miR-138 inhibitor. MTT and migration assays showed that miR-138 inhibitor attenuated the proliferation and migration of smooth muscle cells. Conclusion: In this study, we revealed that miR-138 might promote proliferation and migration of SMC in db/db mice through suppressing the expression of SIRT1. - Highlights: • Higher mRNA level of miR-138 was observed in SMCs from db/db mice. • The mRNA and protein level of SIRT1 in SMCs from db/db mice were greatly reduced. • miR-138 could regulate the expression of SIRT1 in SMCs. • SIRT1 overexpression reversed the up-regulation of acetylized p65 and NF-κB induced by high glucose. • MiR-138 inhibitor reversed VSMCs proliferation and migration induced by high glucose.

  17. miR-206 is down-regulated in breast cancer and inhibits cell proliferation through the up-regulation of cyclinD2

    SciTech Connect (OSTI)

    Zhou, Jing; Tian, Ye; Li, Juan; Lu, Binbin; Sun, Ming; Zou, Yanfen; Kong, Rong; Luo, Yanhong; Shi, Yongguo; Wang, Keming; Ji, Guozhong

    2013-04-05

    Highlights: ? miR-206 was downexpressed in tumor samples compared with matched normal samples. ? Enhanced expression of miR-206 could inhibit breast cancer growth in vitro. ? Luciferase confirmed miR-206 functions as an anti-oncogene by targeting cyclinD2. ? A reverse correlation between miR-206 and cyclinD2 in breast cancer was found. -- Abstract: MicroRNAs act as important gene regulators in human genomes, and their aberrant expression is linked to many malignancies. Aberrant expression of miR-206 has been frequently reported in cancer studies; however, the role and mechanism of its function in breast cancer remains unclear. Quantitative real-time PCR was performed to detect the relative expression levels of miR-206 in breast cancer and normal breast tissues. Lower expression of miR-206 in breast cancer tissues was associated with larger tumour size and a more advanced clinical stage. Further in vitro observations showed that the enforced expression of miR-206 in MCF-7 breast cancer cells inhibited cell growth by blocking the G1/S transition and suppressed cell proliferation and colony formation, implying that miR-206 functions as a tumour suppressor in the progression of breast cancer. Interestingly, Luciferase assays first revealed that miR-206 inhibited cyclinD2 expression by targeting two binding sites in the 3?-untranslated region of cyclinD2 mRNA. qRT-PCR and Western blot assays verified that miR-206 reduced cyclinD2 expression at both the mRNA and protein levels. A reverse correlation between miR-206 and cyclinD2 expression was noted in breast cancer tissues. Altogether, our results identify a crucial tumour suppressive role of miR-206 in the progression of breast cancer, at least partly via up-regulation of the expression of cyclinD2, and suggest that miR-206 might be a candidate prognostic predictor or an anticancer therapeutic target for breast cancer patients.

  18. Validation of the MCNPX-PoliMi Code to Design a Fast-Neutron Multiplicity Counter

    SciTech Connect (OSTI)

    J. L. Dolan; A. C. Kaplan; M. Flaska; S. A. Pozzi; D. L. Chichester

    2012-07-01

    Many safeguards measurement systems used at nuclear facilities, both domestically and internationally, rely on He-3 detectors and well established mathematical equations to interpret coincidence and multiplicity-type measurements for verifying quantities of special nuclear material. Due to resource shortages alternatives to these existing He-3 based systems are being sought. Work is also underway to broaden the capabilities of these types of measurement systems in order to improve current multiplicity analysis techniques. As a part of a Material Protection, Accounting, and Control Technology (MPACT) project within the U.S. Department of Energy's Fuel Cycle Technology Program we are designing a fast-neutron multiplicity counter with organic liquid scintillators to quantify important quantities such as plutonium mass. We are also examining the potential benefits of using fast-neutron detectors for multiplicity analysis of advanced fuels in comparison with He-3 detectors and testing the performance of such designs. The designs are being developed and optimized using the MCNPX-PoliMi transport code to study detector response. In the full paper, we will discuss validation measurements used to justify the use of the MCNPX-PoliMi code paired with the MPPost multiplicity routine to design a fast neutron multiplicity counter with liquid scintillators. This multiplicity counter will be designed with the end goal of safeguarding advanced nuclear fuels. With improved timing qualities associated with liquid scintillation detectors, we can design a system that is less limited by nuclear materials of high activities. Initial testing of the designed system with nuclear fuels will take place at Idaho National Laboratory in a later stage of this collaboration.

  19. T-1025 IU SciBath-768 detector tests in MI-12

    SciTech Connect (OSTI)

    Tayloe, Rex; Cooper, R.; Garrison, L.; Thornton, T.; Rebenitsch, L.; DeJongh, Fritz; Loer, Benjamin; Ramberg, Erik; Yoo, Jonghee; /Fermilab

    2012-02-11

    This is a memorandum of understanding between the Fermi National Accelerator Laboratory (Fermilab) and the experimenters of Department of Physics and Center for Exploration of Energy and Matter, Indiana University, who have committed to participate in detector tests to be carried out during the 2012 Fermilab Neutrino program. The memorandum is intended solely for the purpose of recording expectations for budget estimates and work allocations for Fermilab, the funding agencies and the participating institutions. it reflects an arrangement that currently is satisfactory to the parties; however, it is recognized and anticipated that changing circumstances of the evolving research program will necessitate revisions. The parties agree to modify this memorandum to reflect such required adjustments. Actual contractual obligations will be set forth in separate documents. The experimenters propsoe to test their prototype 'SciBat-768' detector in the MI-12 building for 3 months (February-April) in Spring 2012. The major goal of this effort is to measure or limit the flux of beam-induced neutrons in a far-off-axis (> 45{sup o}) location of the Booster Neutrino Beamline (BNB). This flux is of interest for a proposed coherent neutral-current neutrino-argon elastic scattering experiment. A second goal is to collect more test data for the SciBath-768 to enable better understanding and calibration of the device. The SciBath-768 detector successfully ran for 3 months in the MINOS Underground Area in Fall 2011 as testbeam experiment T-1014 and is currently running above ground in the MINOS service building. For the run proposed here, the experiments are requesting: space in MI-12 in which to run the SciBath detector during February-April 2012 while the BNB is operating; technical support to help with moving the equipment on site; access to power, internet, and accelerator signals; and a small office space from which to run and monitor the experiment.

  20. Ginsenoside-Rg{sub 1} induces angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a

    SciTech Connect (OSTI)

    Kwok, Hoi-Hin; Chan, Lai-Sheung; Poon, Po-Ying; Yue, Patrick Ying-Kit; Wong, Ricky Ngok-Shun

    2015-09-15

    Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. Ginsenoside-Rg{sub 1} (Rg{sub 1}), one of the most abundant active components of ginseng, has been demonstrated as an angiogenesis-stimulating compound in different models. There is increasing evidence implicating microRNAs (miRNAs), a group of non-coding RNAs, as important regulators of angiogenesis, but the role of microRNAs in Rg{sub 1}-induced angiogenesis has not been fully explored. In this report, we found that stimulating endothelial cells with Rg{sub 1} could reduce miR-23a expression. In silico experiments predicted hepatocyte growth factor receptor (MET), a well-established mediator of angiogenesis, as the target of miR-23a. Transfection of the miR-23a precursor or inhibitor oligonucleotides validated the inverse relationship of miR-23a and MET expression. Luciferase reporter assays further confirmed the interaction between miR-23a and the MET mRNA 3′-UTR. Intriguingly, ginsenoside-Rg{sub 1} was found to increase MET protein expression in a time-dependent manner. We further demonstrated that ginsenoside-Rg{sub 1}-induced angiogenic activities were indeed mediated through the down-regulation of miR-23a and subsequent up-regulation of MET protein expression, as confirmed by gain- and loss-of-function angiogenic experiments. In summary, our results demonstrated that ginsenoside-Rg{sub 1} could induce angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a. This study has broadened our understanding of the non-genomic effects of ginsenoside-Rg{sub 1,} and provided molecular evidence that warrant further development of natural compound as novel angiogenesis-promoting therapy. - Highlights: • Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. • Ginsenoside-Rg{sub 1} (Rg{sub 1}) has been demonstrated as an angiogenesis-stimulating compound. • We found that Rg{sub 1} induces angiogenesis by

  1. Analysis of the hydraulic data from the MI fracture zone at the Grimsel Rock Laboratory, Switzerland

    SciTech Connect (OSTI)

    Davey, A.; Karasaki, K.; Long, J.C.S.; Landsfeld, M.; Mensch, A.; Martel, S.J.

    1989-10-01

    One of the major problems in analyzing flow and transport in fractured rock is that the flow may be largely confined to a poorly connected network of fractures. In order to overcome some of this problem, Lawrence Berkeley Laboratory (LBL) has been developing a new type of fracture hydrology model called an equivalent discontinuum model. In this model the authors represent the discontinuous nature of the problem through flow on a partially filled lattice. A key component in constructing an equivalent discontinuum model from this lattice is removing some of the conductive elements such that the system is partially connected in the same manner as the fracture network. This is done through a statistical inverse technique called simulated annealing. The fracture network model is annealed by continually modifying a base model, or template such that the modified systems behave more and more like the observed system. In order to see how the simulated annealing algorithm works, the authors have developed a series of synthetic real cases. In these cases, the real system is completely known so that the results of annealing to steady state data can be evaluated absolutely. The effect of the starting configuration has been studied by varying the percent of conducting elements in the initial configuration. Results have shown that the final configurations converge to about the same percentage of conducting elements. An example using Nagra field data from the Migration Experiment (MI) at Grimsel Rock Laboratory in Switzerland is also analyzed. 24 refs., 33 figs., 3 tabs.

  2. Executive summary of major NuMI lessons learned: a review of relevant meetings of Fermilab's DUSEL Beamline Working Group

    SciTech Connect (OSTI)

    Andrews, Mike; Appel, Jeffrey A.; Bogert, Dixon; Childress, Sam; Cossairt, Don; Griffing, William; Grossman, Nancy; Harding, David; Hylen, Jim; Kuchler, Vic; Laughton, Chris; /Fermilab /Argonne /Brookhaven /LBL, Berkeley

    2009-05-01

    We have gained tremendous experience with the NuMI Project on what was a new level of neutrino beams from a high power proton source. We expect to build on that experience for any new long baseline neutrino beam. In particular, we have learned about some things which have worked well and/or where the experience is fairly directly applicable to the next project (e.g., similar civil construction issues including: tunneling, service buildings, outfitting, and potential claims/legal issues). Some things might be done very differently (e.g., decay pipe, windows, target, beam dump, and precision of power supply control/monitoring). The NuMI experience does lead to identification of critical items for any future such project, and what issues it will be important to address. The DUSEL Beamline Working Group established at Fermilab has been meeting weekly to collect and discuss information from that NuMI experience. This document attempts to assemble much of that information in one place. In this Executive Summary, we group relevant discussion of some of the major issues and lessons learned under seven categories: (1) Differences Between the NuMI Project and Any Next Project; (2) The Process of Starting Up the Project; (3) Decision and Review Processes; (4) ES&H: Environment, Safety, and Health; (5) Local Community Buy-In; (6) Transition from Project Status to Operation; and (7) Some Lessons on Technical Elements. We concentrate here on internal project management issues, including technical areas that require special attention. We cannot ignore, however, two major external management problems that plagued the NuMI project. The first problem was the top-down imposition of an unrealistic combination of scope, cost, and schedule. This situation was partially corrected by a rebaselining. However, the full, desirable scope was never achievable. The second problem was a crippling shortage of resources. Critical early design work could not be done in a timely fashion, leading to

  3. Mitsubishi iMiEV: An Electric Mini-Car in NREL's Advanced Technology Vehicle Fleet (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2011-10-01

    This fact sheet highlights the Mitsubishi iMiEV, an electric mini-car in the advanced technology vehicle fleet at the National Renewable Energy Laboratory (NREL). In support of the U.S. Department of Energy's fast-charging research efforts, NREL engineers are conducting charge and discharge performance testing on the vehicle. NREL's advanced technology vehicle fleet features promising technologies to increase efficiency and reduce emissions without sacrificing safety or comfort. The fleet serves as a technology showcase, helping visitors learn about innovative vehicles that are available today or are in development. Vehicles in the fleet are representative of current, advanced, prototype, and emerging technologies.

  4. shaleusa5.pdf

    Gasoline and Diesel Fuel Update (EIA)

    ... NY PA NJ OH DC DE WV MD KY VA NC TN SC MI CANADA 2 0 0 1 0 0 Marcellus Shale Gas Play, Appalachian Basin Source: US Energy Information Administration based on data from WVGES , PA ...

  5. Evaluation of Multiplexed 16S rRNA Microbial Population Surveys Using Illumina MiSeq Platform (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema (OSTI)

    Tremblay, Julien [DOE JGI

    2013-01-25

    Julien Tremblay from DOE JGI presents "Evaluation of Multiplexed 16S rRNA Microbial Population Surveys Using Illumina MiSeq Platorm" at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  6. A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nagarkar-Jaiswal, Sonal; Lee, Pei-Tseng; Campbell, Megan E.; Chen, Kuchuan; Anguiano-Zarate, Stephanie; Cantu Gutierrez, Manuel; Busby, Theodore; Lin, Wen-Wen; He, Yuchun; Schulze, Karen L.; et al

    2015-03-31

    Here, we document a collection of ~7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstratemore » reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.« less

  7. A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

    SciTech Connect (OSTI)

    Nagarkar-Jaiswal, Sonal; Lee, Pei-Tseng; Campbell, Megan E.; Chen, Kuchuan; Anguiano-Zarate, Stephanie; Cantu Gutierrez, Manuel; Busby, Theodore; Lin, Wen-Wen; He, Yuchun; Schulze, Karen L.; Booth, Benjamin W.; Evans-Holm, Martha; Venken, Koen J.T.; Levis, Robert W.; Spradling, Allan C.; Hoskins, Roger A.; Bellen, Hugo J.

    2015-03-31

    Here, we document a collection of ~7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.

  8. Repression of miR-17-5p with elevated expression of E2F-1 and c-MYC in non-metastatic hepatocellular carcinoma and enhancement of cell growth upon reversing this expression pattern

    SciTech Connect (OSTI)

    El Tayebi, H.M.; Omar, K.; Hegy, S.; El Maghrabi, M.; El Brolosy, M.; Hosny, K.A.; Esmat, G.; Abdelaziz, A.I.

    2013-05-10

    Highlights: The oncogenic miR-17-5p is downregulated in non-metastatic hepatocellular carcinoma patients. E2F-1 and c-MYC transcripts are upregulated in non-metastatic HCC patients. miR-17-5p forced overexpression inhibited E2F-1 and c-MYC expression in HuH-7 cells. miR-17-5p mimicking increased HuH-7 cell growth, proliferation, migration and colony formation. miR-17-5p is responsible for HCC progression among the c-MYC/E2F-1/miR-17-5p triad members. -- Abstract: E2F-1, c-MYC, and miR-17-5p is a triad of two regulatory loops: a negative and a positive loop, where c-MYC induces the expression of E2F-1 that induces the expression of miR-17-5p which in turn reverses the expression of E2F-1 to close the loop. In this study, we investigated this triad for the first time in hepatocellular carcinoma (HCC), where miR-17-5p showed a significant down-regulation in 23 non-metastatic HCC biopsies compared to 10 healthy tissues; however, E2F-1 and c-MYC transcripts were markedly elevated. Forced over-expression of miR-17-5p in HuH-7 cells resulted in enhanced cell proliferation, growth, migration and clonogenicity with concomitant inhibition of E2F-1 and c-MYC transcripts expressions, while antagomirs of miR-17-5p reversed these events. In conclusion, this study revealed a unique pattern of expression for miR-17-5p in non-metastatic HCC patients in contrast to metastatic HCC patients. In addition we show that miR-17-5p is the key player among the triad that tumor growth and spread.

  9. Resonances in Coupled <mimi><mi>Kmi>-<mi>ηK> Scattering from Quantum Chromodynamics

    SciTech Connect (OSTI)

    Dudek, Jozef J.; Edwards, Robert G.; Thomas, Christopher E.; Wilson, David J.

    2014-10-01

    Using first-principles calculation within Quantum Chromodynamics, we are able to reproduce the pattern of experimental strange resonances which appear as complex singularities within coupled πK, ηK scattering amplitudes. We make use of numerical computation within the lattice discretized approach to QCD, extracting the energy dependence of scattering amplitudes through their relation- ship to the discrete spectrum of the theory in a finite-volume, which we map out in unprecedented detail.

  10. A study of muon neutrino disappearance with the MINOS detectors and the NuMI neutrino beam

    SciTech Connect (OSTI)

    Marshall, John Stuart; /Cambridge U.

    2008-06-01

    This thesis presents the results of an analysis of {nu}{sub {mu}} disappearance with the MINOS experiment, which studies the neutrino beam produced by the NuMI facility at Fermi National Accelerator Laboratory. The rates and energy spectra of charged current {nu}{sub {mu}} interactions are measured in two similar detectors, located at distances of 1 km and 735 km along the NuMI beamline. The Near Detector provides accurate measurements of the initial beam composition and energy, while the Far Detector is sensitive to the effects of neutrino oscillations. The analysis uses data collected between May 2005 and March 2007, corresponding to an exposure of 2.5 x 10{sup 20} protons on target. As part of the analysis, sophisticated software was developed to identify muon tracks in the detectors and to reconstruct muon kinematics. Events with reconstructed tracks were then analyzed using a multivariate technique to efficiently isolate a pure sample of charged current {nu}{sub {mu}} events. An extrapolation method was also developed, which produces accurate predictions of the Far Detector neutrino energy spectrum, based on data collected at the Near Detector. Finally, several techniques to improve the sensitivity of an oscillation measurement were implemented, and a full study of the systematic uncertainties was performed. Extrapolating from observations at the Near Detector, 733 {+-} 29 Far Detector events were expected in the absence of oscillations, but only 563 events were observed. This deficit in event rate corresponds to a significance of 4.3 standard deviations. The deficit is energy dependent and clear distortion of the Far Detector energy spectrum is observed. A maximum likelihood analysis, which fully accounts for systematic uncertainties, is used to determine the allowed regions for the oscillation parameters and identifies the best fit values as {Delta}m{sub 32}{sup 2} = 2.29{sub -0.14}{sup +0.14} x 10{sup -3} eV{sup 2} and sin{sup 2} 2{theta}{sub 23} > 0