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  1. Fossil Gulch Wind Park | Open Energy Information

    Open Energy Info (EERE)

    Gulch Wind Park Jump to: navigation, search Name Fossil Gulch Wind Park Facility Fossil Gulch Wind Park Sector Wind energy Facility Type Commercial Scale Wind Facility Status In...

  2. DOE - Office of Legacy Management -- Estes Gulch Disposal Cell...

    Office of Legacy Management (LM)

    Estes Gulch Disposal Cell - 010 FUSRAP Considered Sites Site: Estes Gulch Disposal Cell (010) Designated Name: Alternate Name: Location: Evaluation Year: Site Operations: Site ...

  3. hare-98.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Diurnal Variability of Turbulent Fluxes in the Equatorial Pacific J. E. Hare Cooperative Institute for Research in Environmental Sciences University of Colorado Boulder, Colorado C. W. Fairall NOAA-Environmental Technology Laboratory Boulder, Colorado Introduction During March and April of 1996, scientists from 13 national research institutions participated in the Combined Sensor Program (CSP) experiment. This program is a collaborative effort of the U.S. Department of Energy (DOE) and the

  4. HARE: Final Report

    SciTech Connect (OSTI)

    Mckie, Jim

    2012-01-09

    This report documents the results of work done over a 6 year period under the FAST-OS programs. The first effort was called Right-Weight Kernels, (RWK) and was concerned with improving measurements of OS noise so it could be treated quantitatively; and evaluating the use of two operating systems, Linux and Plan 9, on HPC systems and determining how these operating systems needed to be extended or changed for HPC, while still retaining their general-purpose nature. The second program, HARE, explored the creation of alternative runtime models, building on RWK. All of the HARE work was done on Plan 9. The HARE researchers were mindful of the very good Linux and LWK work being done at other labs and saw no need to recreate it. Even given this limited funding, the two efforts had outsized impact: _ Helped Cray decide to use Linux, instead of a custom kernel, and provided the tools needed to make Linux perform well _ Created a successor operating system to Plan 9, NIX, which has been taken in by Bell Labs for further development _ Created a standard system measurement tool, Fixed Time Quantum or FTQ, which is widely used for measuring operating systems impact on applications _ Spurred the use of the 9p protocol in several organizations, including IBM _ Built software in use at many companies, including IBM, Cray, and Google _ Spurred the creation of alternative runtimes for use on HPC systems _ Demonstrated that, with proper modifications, a general purpose operating systems can provide communications up to 3 times as effective as user-level libraries Open source was a key part of this work. The code developed for this project is in wide use and available at many places. The core Blue Gene code is available at https://bitbucket.org/ericvh/hare. We describe details of these impacts in the following sections. The rest of this report is organized as follows: First, we describe commercial impact; next, we describe the FTQ benchmark and its impact in more detail; operating

  5. AmeriFlux US-Wkg Walnut Gulch Kendall Grasslands

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Scott, Russell [United States Department of Agriculture

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-Wkg Walnut Gulch Kendall Grasslands. Site Description - This site is located in a small, intensively-studied, experimental watershed within USDA-ARS's Walnut Gulch Experimental Watershed. Eddy covariance measurements of energy, water and CO2 fluxes began in the spring of 2004, though meteorological (including Bowen ratio) and hydrological measurements are available much further back.

  6. DOE - Office of Legacy Management -- Estes Gulch Disposal Cell - 010

    Office of Legacy Management (LM)

    Estes Gulch Disposal Cell - 010 FUSRAP Considered Sites Site: Estes Gulch Disposal Cell (010) Remediated; managed by DOE LM. More information at http://www.lm.doe.gov/Rifle/Disposal/Sites.aspx Designated Name: Not Designated under FUSRAP Alternate Name: Rifle, CO, Disposal Site Location: Rifle, Colorado Evaluation Year: Not considered for FUSRAP - in another program Site Operations: Uranium mill tailings disposal Site Disposition: Remediated under UMTRCA Title I Radioactive Materials Handled:

  7. Floodplain and Wetlands Assessment for Construction of a Second Full Service Access Road over Lena Gulch

    Broader source: Energy.gov [DOE]

    Floodplain and Wetlands Assessment for Construction of a Second Full Service Access Road over Lena Gulch from South Golden Road to the National Renewable Energy Laboratory’s South Table Mountain Complex, Golden, Colorado.

  8. Geologic map and coal resources of the Easton Gulch Quadrangle, Moffat County, Colorado

    SciTech Connect (OSTI)

    Reheis, M.C.

    1981-01-01

    This map of the Easton Gulch Quadrangle, Moffat County, Colorado is color coded to show the location of different age geologic formations. Various thickness coal bed are indicated as are abandoned coal mines or prospects, US Geologic Survey (USGS) test holes, abandoned oil and gas test holes, and USGS Mesozoic fossil localities. Various depth coal beds and other types of geologic structures are indicated on the cross-section geologic map. (BLM)

  9. Preliminary assessment report for Waiawa Gulch, Installation 15080, Pearl City, Oahu, Hawaii. Installation Restoration Program

    SciTech Connect (OSTI)

    Not Available

    1993-08-01

    This report presents the results of the preliminary assessment (PA) conducted by Argonne National Laboratory at the Hawaii Army National Guard (HIARNG) property near Pearl City, Oahu, Hawaii. Preliminary assessments of federal facilities are being conducted to compile the information necessary for completing preremedial activities and to provide a basis for establishing corrective actions in response to releases of hazardous substances. The principal objective of the PA is to characterize the site accurately and determine the need for further action by examining site activities, quantities of hazardous substances present, and potential pathways by which contamination could affect public health and the environment. This PA satisfies, for the Waiawa Gulch property, phase I of the Department of Defense Installation Restoration Program (IRP).

  10. Environmental assessment for the granting of US Navy easements near Pearl Harbor and Waikele Gulch Oahu, Hawaii to Hawaiian Electric Company, Inc. Final report

    SciTech Connect (OSTI)

    Luersen, P.; Wiley, M.; Hart, J.; Kanehiro, C.; Soloway, M.

    1994-09-23

    This document was prepared pursuant to the National Environmental Policy Act (NEPA: and Navy regulations (OPNAVINST 5090.1B)). The document addresses environmental impacts from the proposed granting of three easements to the Hawaiian Electric Company, Inc. (HECO) for the use of Navy property near Waikele Gulch, Pearl City and the Waiau Power Plant. HECO`s proposed Waiau-CIP, Part 2, Project requires installation, operation, and maintenance of steel transmission line poles and associated equipment on three Navy parcels crossed by the 7.8 mile project. Health risks from exposure to the electric and magnetic fields of 138-kV transmission lines are discussed.

  11. Wild Rose Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    Wild Rose Geothermal Area Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Wild Rose Geothermal Area Contents 1 Area Overview 2 History and Infrastructure 3 Regulatory...

  12. WILD PIG ATTACKS ON HUMANS

    SciTech Connect (OSTI)

    Mayer, J.

    2013-04-12

    Attacks on humans by wild pigs (Sus scrofa) have been documented since ancient times. However, studies characterizing these incidents are lacking. In an effort to better understand this phenomenon, information was collected from 412 wild pig attacks on humans. Similar to studies of large predator attacks on humans, data came from a variety of sources. The various attacks compiled occurred in seven zoogeographic realms. Most attacks occurred within the species native range, and specifically in rural areas. The occurrence was highest during the winter months and daylight hours. Most happened under non-hunting circumstances and appeared to be unprovoked. Wounded animals were the chief cause of these attacks in hunting situations. The animals involved were typically solitary, male and large in size. The fate of the wild pigs involved in these attacks varied depending upon the circumstances, however, most escaped uninjured. Most human victims were adult males traveling on foot and alone. The most frequent outcome for these victims was physical contact/mauling. The severity of resulting injuries ranged from minor to fatal. Most of the mauled victims had injuries to only one part of their bodies, with legs/feet being the most frequent body part injured. Injuries were primarily in the form of lacerations and punctures. Fatalities were typically due to blood loss. In some cases, serious infections or toxemia resulted from the injuries. Other species (i.e., pets and livestock) were also accompanying some of the humans during these attacks. The fates of these animals varied from escaping uninjured to being killed. Frequency data on both non-hunting and hunting incidents of wild pig attacks on humans at the Savannah River Site, South Carolina, showed quantitatively that such incidents are rare.

  13. NREL: Wind-Wildlife Impacts Literature Database (WILD) Home Page

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NREL - National Renewable Energy Laboratory Wind-Wildlife Impacts Literature Database (WILD) Wind Research WILD WILD Browse By Reset All Geography Africa (45) Apply Africa filter...

  14. Hog wild | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hog wild Hog wild Posted: May 7, 2014 - 5:28pm | Y-12 Report | Volume 10, Issue 2 | 2014 Tennessee inventor Jake Gish, winner of the third annual Y-12 Innovation Competition, has it out for feral hogs, and with good reason. Originally found across much of Europe and Asia, the beasts, which are the size of small bears, have wreaked havoc on humans since at least the Middle Ages. The wild boars have since expanded their territory and in the past few decades have become increasingly prevalent - and

  15. Interagency Wild and Scenic Rivers Coordinating Council's WSRA...

    Open Energy Info (EERE)

    to use the flowcharts created by the Interagency Wild and Scenic Rivers Coordinating Council. Author The Interagency Wild and Scenic Rivers Coordinating Council Published The...

  16. Wild Horse Wind Power Project | Open Energy Information

    Open Energy Info (EERE)

    Wind Power Project Jump to: navigation, search Name Wild Horse Wind Power Project Facility Wild Horse Wind Power Project Sector Wind energy Facility Type Commercial Scale Wind...

  17. Chemical analysis of Wild-2 samples returned by Stardust (Conference...

    Office of Scientific and Technical Information (OSTI)

    Conference: Chemical analysis of Wild-2 samples returned by Stardust Citation Details In-Document Search Title: Chemical analysis of Wild-2 samples returned by Stardust Authors: ...

  18. Wild and Scenic Rivers Act (1968) | Department of Energy

    Office of Environmental Management (EM)

    Wild and Scenic Rivers Act (1968) The National Wild and Scenic Rivers System was created by Congress in 1968 (Public Law 90-542; 16 U.S.C. 1271 et seq.) to preserve certain rivers ...

  19. Wild Horse II Wind Farm | Open Energy Information

    Open Energy Info (EERE)

    II Wind Farm Jump to: navigation, search Name Wild Horse II Wind Farm Facility Wild Horse II Sector Wind energy Facility Type Commercial Scale Wind Facility Status In Service Owner...

  20. DOE - NNSA/NFO -- Photo Library Wild Flowers

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wild Flowers NNSA/NFO Language Options U.S. DOE/NNSA - Nevada Field Office Photo Library - Wild Flowers Wild flowers such as Cane Spring suncup and White bearpoppy are included among the 750 varieties of plants located on the Nevada National Security Site. Instructions: Click the photograph THUMBNAIL to view the photograph details Thumbnail Category http://www.nv.doe.gov/library/photos/thumbs/CaneSpringsuncup.jpg Wild Flowers http://www.nv.doe.gov/library/photos/thumbs/Clokeys.jpg Wild Flowers

  1. Wild and Scenic Rivers Act | Open Energy Information

    Open Energy Info (EERE)

    as wild, scenic, or recreational. These classifications necessitate differing levels of environmental protection under the Act. Published NA Year Signed or Took Effect 1968...

  2. Technical Report of the Wild and Scenic Rivers Coordinating Council...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: Technical Report of the Wild and Scenic Rivers Coordinating...

  3. V-143: Fresh Java issues being exploited in the wild

    Broader source: Energy.gov [DOE]

    Java issues are being exploited in the wild by exploit kits, with Cool and Redkit specifically being known to use these bugs, and others likely to follow shortly.

  4. The antitrust wild card and electricity restructuring

    SciTech Connect (OSTI)

    Adelberg, A.W.; Ongman, J.W.

    1997-03-01

    If competitive policy issues in electricity restructuring are not addressed soon--preferably in federal legislation--it`s likely that someone will use the antitrust wild card to achieve its ends. Experience teaches that this may not be the best way to make public policy. As the electric utility industry restructures, it is widely assumed that Congress, the state legislatures, and regulators will set the ground rules for the restructured markets. Experience to date would seem to confirm this view: California`s restructuring legislation, FERC`s Order 888, and the restructuring proceedings in numerous states are all examples. And yet, there remains another player whose role could be equally important: The federal judiciary. While court decisions under the antitrust laws have had little influence to date on the industry`s direction, there is reason to believe that their role could increase dramatically. Certainly this is the history of other industries that have undergone similar transformations. The authors expect that forces at work in the electric utility industry could lead to antitrust actions playing a far greater role in the industry`s future than most observers currently expect. The electric utility industry has already experienced a close brush with the potential for antitrust rulings to unravel critical elements of regulatory policy on restructuring. The DC Circuit`s now famous (or infamous) dicta in the Cajun Electric Power case illustrated how a simple antitrust principle--the prohibition on so-called tying arrangements--could defeat the FERC`s policy with respect to utilities` recovery of billions of dollars of stranded costs. The FERC rebutted that dicta in its remand decisions and elsewhere, and it appears that the issue in now moot in the Cajun litigation itself. But the tying arrangement argument is far from dead.

  5. WILD PIGS: BIOLOGY, DAMAGE, CONTROL TECHINQUES AND MANAGEMENT

    SciTech Connect (OSTI)

    Mayer, John; Brisbin, I. Lehr

    2009-12-31

    The existence of problems with wild pigs (Sus scrofa) is nothing new to the Western Hemisphere. Damage by these introduced animals was reported as far back as 1505 by the early Spanish colonies in the Caribbean, where wild pigs were killing the colonists cattle. Droves of these animals also ravaged cultivated crops of maize and sugarcane on islands in the West Indies during this same time period. These wild pigs reportedly were very aggressive and often attacked Spanish soldiers hunting rebellious Indians or escaped slaves on these islands, especially when these animals were cornered. The documentation of such impacts by introduced populations of this species in the United States has subsequently increased in recent years, and continued up through the present (Towne and Wentworth. 1950, Wood and Barrett 1979, Mayer and Brisbin 1991, Dickson et al. 2001). In spite of a fairly constant history in this country since the early 1900s, wild pigs have had a dramatic recent increase in both distribution and numbers in the United States. Between 1989 and 2009, the number of states reporting the presence of introduced wild pigs went from 19 up to as many as 44. This increase, in part natural, but largely manmade, has caused an increased workload and cost for land and resource managers in areas where these new populations are found. This is the direct result of the damage that these introduced animals do. The cost of both these impacts and control efforts has been estimated to exceed a billion dollars annually (Pimentel 2007). The complexity of this problem has been further complicated by the widespread appeal and economic potential of these animals as a big game species (Tisdell 1982, Degner 1989). Wild pigs are a controversial problem that is not going away and will likely only get worse with time. Not only do they cause damage, but wild pigs are also survivors. They reproduce at a rate faster than any other mammal of comparable size, native or introduced; they can eat just

  6. Wind-Wildlife Impacts Literature Database (WILD)(Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2015-01-01

    The Wind-Wildlife Impacts Literature Database (WILD), developed and maintained by the National Wind Technology Center (NWTC) at the National Renewable Energy Laboratory (NREL), is comprised of over 1,000 citations pertaining to the effects of land-based wind, offshore wind, marine and hydrokinetic, power lines, and communication and television towers on wildlife.

  7. COLLOQUIUM: A Wild Solution for Climate Change | Princeton Plasma Physics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lab 15, 2015, 2:00pm to 3:30pm Colloquia MBG Auditorium COLLOQUIUM: A Wild Solution for Climate Change Professor Thomas E. Lovejoy George Mason University Presentation: PDF icon WC15APR2015_TELovejoy.pdf The impacts of climate change on biodiversity and nature is increasingly prevalent. Projected ahead climate change has increasingly greater impacts beyond what the Climate Convention refers to as allowing systems to adapt naturally. The presentation will examine the two degree target for

  8. Wind-Wildlife Impacts Literature Database (WILD)(Fact Sheet), NREL (National Renewable Energy Laboratory)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wind-Wildlife Impacts Literature Database (WILD) What is WILD? The Wind-Wildlife Impacts Literature Database (WILD), developed and main- tained by the National Wind Technology Center (NWTC) at the National Renewable Energy Laboratory (NREL), is comprised of over 1,000 citations pertaining to the effects of land-based wind, offshore wind, marine and hydrokinetic power systems, power lines, and communication and television towers on wildlife. For the wind energy sector, WILD serves as an

  9. Transgenic crops get a test in the wild

    SciTech Connect (OSTI)

    Cherfas, J.

    1991-02-22

    A novel British research program called PROSAMO - Planned Release of Selected and Modified Organisms - has just produced its first batch of results on the ecological behavior of a genetically manipulated variety of oil seed rape (known to Americans as canola). As expected, the preliminary data indicate that these plants do not outgrow their competitors in the wild, nor is there any evidence that they pass on their foreign genes to other species. PROSAMO is moving on to test other crops with other foreign genes. If these results are as reassuring, scientists around the world will have solid evidence with which to soothe fears.

  10. Wild Horse 69-kV transmission line environmental assessment

    SciTech Connect (OSTI)

    1996-12-01

    Hill County Electric Cooperative Inc. (Hill County) proposes to construct and operate a 69-kV transmission line from its North Gildford Substation in Montana north to the Canadian border. A vicinity project area map is enclosed as a figure. TransCanada Power Corporation (TCP), a Canadian power-marketing company, will own and construct the connecting 69-kV line from the international border to Express Pipeline`s pump station at Wild Horse, Alberta. This Environmental Assessment is prepared for the Department of Energy (DOE) as lead federal agency to comply with the requirements of the National Environmental Policy Act (NEPA), as part of DOE`s review and approval process of the applications filed by Hill County for a DOE Presidential Permit and License to Export Electricity to a foreign country. The purpose of the proposed line is to supply electric energy to a crude oil pump station in Canada, owned by Express Pipeline Ltd. (Express). The pipeline would transport Canadian-produced oil from Hardisty, Alberta, Canada, to Caster, Wyoming. The Express Pipeline is scheduled to be constructed in 1996--97 and will supply crude oil to refineries in Wyoming and the midwest.

  11. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print Wednesday, 30 May 2007 00:00 NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination

  12. WAC - 232-12-064 Live Wildlife-Taking from the wild | Open Energy...

    Open Energy Info (EERE)

    32-12-064 Live Wildlife-Taking from the wild Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 232-12-064 Live...

  13. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wild 2 Viewed by ALS Microscopes Print NASA's 200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81PWild...

  14. Title 36 CFR 297 Wild and Scenic Rivers | Open Energy Information

    Open Energy Info (EERE)

    Abstract Section 7 of the Wild and Scenic Rivers Act provides for the protection of the free-flowing, scenic, and natural values of rivers designated as components or potential...

  15. Title 36 C.F.R. 297 Wild and Scenic Rivers | Open Energy Information

    Open Energy Info (EERE)

    Abstract Section 7 of the Wild and Scenic Rivers Act provides for the protection of the free-flowing, scenic, and natural values of rivers designated as components or potential...

  16. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  17. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  18. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  19. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  20. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  1. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  2. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes Print NASA's $200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81P/Wild 2. Four ALS beamlines and the researchers using them were among the hundreds of scientists and dozens of experimental techniques in facilities around the world that contributed to the preliminary examination of the first samples. Adding to recent advances in cometary science showing the

  3. Stability of Iowa mutant and wild type Aβ-peptide aggregates

    SciTech Connect (OSTI)

    Alred, Erik J.; Scheele, Emily G.; Berhanu, Workalemahu M.; Hansmann, Ulrich H. E.

    2014-11-07

    Recent experiments indicate a connection between the structure of amyloid aggregates and their cytotoxicity as related to neurodegenerative diseases. Of particular interest is the Iowa Mutant, which causes early-onset of Alzheimer's disease. While wild-type Amyloid β-peptides form only parallel beta-sheet aggregates, the mutant also forms meta-stable antiparallel beta sheets. Since these structural variations may cause the difference in the pathological effects of the two Aβ-peptides, we have studied in silico the relative stability of the wild type and Iowa mutant in both parallel and antiparallel forms. We compare regular molecular dynamics simulations with such where the viscosity of the samples is reduced, which, we show, leads to higher sampling efficiency. By analyzing and comparing these four sets of all-atom molecular dynamics simulations, we probe the role of the various factors that could lead to the structural differences. Our analysis indicates that the parallel forms of both wild type and Iowa mutant aggregates are stable, while the antiparallel aggregates are meta-stable for the Iowa mutant and not stable for the wild type. The differences result from the direct alignment of hydrophobic interactions in the in-register parallel oligomers, making them more stable than the antiparallel aggregates. The slightly higher thermodynamic stability of the Iowa mutant fibril-like oligomers in its parallel organization over that in antiparallel form is supported by previous experimental measurements showing slow inter-conversion of antiparallel aggregates into parallel ones. Knowledge of the mechanism that selects between parallel and antiparallel conformations and determines their relative stability may open new avenues for the development of therapies targeting familial forms of early-onset Alzheimer's disease.

  4. Ecological interactions between hatchery summer steelhead and wild Oncorhynchus mykiss in the Willamette River basin, 2014

    SciTech Connect (OSTI)

    Harnish, Ryan A.; Green, Ethan D.; Vernon, Christopher R.; Mcmichael, Geoffrey A.

    2014-12-23

    The purpose of this study was to determine the extent to which juvenile hatchery summer steelhead and wild winter steelhead overlap in space and time, to evaluate the extent of residualism among hatchery summer steelhead in the South Santiam River, and to evaluate the potential for negative ecological interactions among hatchery summer steelhead and wild winter steelhead. Because it is not possible to visually discern juvenile winter steelhead from resident rainbow trout, we treated all adipose-intact juvenile O. mykiss as one group that represented juvenile wild winter steelhead. The 2014 study objectives were to 1) estimate the proportion of hatchery summer steelhead that residualized in the South Santiam River in 2014, 2) determine the extent to which hatchery and naturally produced O. mykiss overlapped in space and time in the South Santiam River, and 3) characterize the behavioral interactions between hatchery-origin juvenile summer steelhead and naturally produced O. mykiss. We used a combination of radio telemetry and direct observations (i.e., snorkeling) to determine the potential for negative interactions between hatchery summer and wild winter steelhead juveniles in the South Santiam River. Data collected from these two independent methods indicated that a significant portion of the hatchery summer steelhead released as smolts did not rapidly emigrate from the South Santiam River in 2014. Of the 164 radio-tagged steelhead that volitionally left the hatchery, only 66 (40.2%) were detected outside of the South Santiam River. Forty-four (26.8% of 164) of the radio-tagged hatchery summer steelhead successfully emigrated to Willamette Falls. Thus, the last known location of the majority of the tagged fish (98 of 164 = 59.8%) was in the South Santiam River. Thirty-three of the tagged hatchery steelhead were detected in the South Santiam River during mobile-tracking surveys. Of those, 21 were found to be alive in the South Santiam River over three months after

  5. Wood-Producing Sunflower? Mining Genetic Diversity in Desert-Dwelling Wild Species (2010 JGI User Meeting)

    ScienceCinema (OSTI)

    Knapp, Steve

    2011-04-26

    Steve Knapp from Monsanto on "Wood-Producing Sunflower? Mining Genetic Diversity in Desert-Dwelling Wild Species" on March 25, 2010 at the 5th Annual DOE JGI User Meeting

  6. Studies Concerning the Accumulation of Minerals and Heavy Metals in Fruiting Bodies of Wild Mushrooms

    SciTech Connect (OSTI)

    Stihi, Claudia; Radulescu, Cristiana; Gheboianu, Anca; Bancuta, Iulian; Popescu, Ion V.; Busuioc, Gabriela

    2011-10-03

    The minerals and heavy metals play an important role in the metabolic processes, during the growth and development of mushrooms, when they are available in appreciable concentration. In this work the concentrations of Cr, Mn, Fe, Ni, Cu, Zn, Se, Cd and Pb were analyzed using the Flame Atomic Absorption spectrometry (FAAS) together with Energy Dispersive X-ray Fluorescence spectrometry (EDXRF) in 3 wild mushrooms species and their growing substrate, collected from various forestry fields in Dambovita County, Romania. The analyzed mushrooms were: Amanita phalloides, Amanita rubescens and Armillariella mellea. The accumulation coefficients were calculated to assess the mobility of minerals and heavy metals from substrate to mushrooms [1].

  7. Science on Saturday: The Wild and Wacky World of Epigenetics | Princeton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plasma Physics Lab 6, 2016, 9:30am Science On Saturday PPPL, MBG Auditorium Science on Saturday: The Wild and Wacky World of Epigenetics Professor Shirley Tilghman Princeton University Abstract: PDF icon 02 Tilghman.pdf LIVE STREAMING LINK: https://mediacentral.princeton.edu/id/1_wdp1m3et Tweet us your questions @PPPLSciEd and/or #scionsat Science_on_Saturday16Jan2016_STilghman Contact Information Coordinator(s): Ms. Deedee Ortiz-Arias dortiz@pppl.gov Host(s): Dr. Andrew Zwicker

  8. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E.

    2015-11-28

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimummore » detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg-1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg-1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.« less

  9. Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

    SciTech Connect (OSTI)

    Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E.

    2015-11-28

    In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimum detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg-1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg-1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation

  10. Digital Mapping and Environmental Characterization of National Wild and Scenic River Systems

    SciTech Connect (OSTI)

    McManamay, Ryan A; Bosnall, Peter; Hetrick, Shelaine L; Smith, Brennan T

    2013-09-01

    Spatially accurate geospatial information is required to support decision-making regarding sustainable future hydropower development. Under a memorandum of understanding among several federal agencies, a pilot study was conducted to map a subset of National Wild and Scenic Rivers (WSRs) at a higher resolution and provide a consistent methodology for mapping WSRs across the United States and across agency jurisdictions. A subset of rivers (segments falling under the jurisdiction of the National Park Service) were mapped at a high resolution using the National Hydrography Dataset (NHD). The spatial extent and representation of river segments mapped at NHD scale were compared with the prevailing geospatial coverage mapped at a coarser scale. Accurately digitized river segments were linked to environmental attribution datasets housed within the Oak Ridge National Laboratory s National Hydropower Asset Assessment Program database to characterize the environmental context of WSR segments. The results suggest that both the spatial scale of hydrography datasets and the adherence to written policy descriptions are critical to accurately mapping WSRs. The environmental characterization provided information to deduce generalized trends in either the uniqueness or the commonness of environmental variables associated with WSRs. Although WSRs occur in a wide range of human-modified landscapes, environmental data layers suggest that they provide habitats important to terrestrial and aquatic organisms and recreation important to humans. Ultimately, the research findings herein suggest that there is a need for accurate, consistent, mapping of the National WSRs across the agencies responsible for administering each river. Geospatial applications examining potential landscape and energy development require accurate sources of information, such as data layers that portray realistic spatial representations.

  11. The Origin of Refractory Minerals in Comet 81P/Wild 2

    SciTech Connect (OSTI)

    Chi, M; Ishii, H A; Simon, S B; Bradley, J P; Dai, Z R; Joswiak, D J; Browning, N D; Matrajt, G

    2008-11-20

    Refractory Ti-bearing minerals in the calcium-, aluminium-rich inclusion (CAI) Inti, recovered from the comet 81P/Wild 2 sample, were examined using analytical (scanning) transmission electron microscopy (STEM) methods including imaging, nanodiffraction, energy dispersive spectroscopy (EDX) and electron energy loss spectroscopy (EELS). Inti fassaite (Ca(Mg,Ti,Al)(Si,Al){sub 2}O{sub 6}) was found to have a Ti{sup 3+}/Ti{sup 4+} ratio of 2.0 {+-} 0.2, consistent with fassaite in other solar system CAIs. The oxygen fugacity (log f{sub O{sub 2}}) of formation estimated from this ratio, assuming equilibration among phases at 1509K, is -19.4 {+-} 1.3. This value is near the canonical solar nebula value (-18.1 {+-} 0.3) and in close agreement with that reported for fassaite-bearing Allende CAIs (-19.8 {+-} 0.9) by other researchers using the same assumptions. Nanocrystals of osbornite (Ti(V)N), 2-40 nm in diameter, are embedded as inclusions within anorthite, spinel and diopside in Inti. Vanadium is heterogeneously distributed within some osbornite crystals. Compositions range from pure TiN to Ti{sub 0.36}V{sub 0.64}N. The possible presence of oxide and carbide in solid solution with the osbornite was evaluated. The osbornite may contain O but does not contain C. The presence of osbornite, likely a refractory early condensate, together with the other refractory minerals in Inti, indicates that the parent comet contains solids that condensed closer to the proto-sun than the distance at which the parent comet itself accreted. The estimated oxygen fugacity and the reported isotopic and chemical compositions are consistent with Inti originating in the inner solar system as opposed to it being a surviving CAI from an extrasolar source. These results provide insight for evaluating the validity of models of radial mass transport dynamics in the early solar system. The oxidation environments inferred for the Inti mineral assemblage are inconsistent with an X-wind formation

  12. A Refractory Inclusion Returned by Stardust from Comet 81P/Wild 2

    SciTech Connect (OSTI)

    Simon, S B; Joswiak, D J; Ishii, H A; Bradley, J P; Chi, M; Grossman, L; Al?on, J; Brownlee, D E; Fallon, S; Hutcheon, I D; Matrajt, G; McKeegan, K D

    2008-05-20

    Among the samples returned from comet 81P/Wild 2 by the Stardust spacecraft is a suite of particles from one impact track (Track 25) that are Ca-, Al-rich and FeO-free. We studied three particles from this track that range in size from 5.3 x 3.2 {micro}m to 15 x 10 {micro}m. Scanning and transmission electron microscopy show that they consist of very fine-grained (from {approx}0.5 to {approx}2 {micro}m) Al-rich, Ti-bearing and Ti-free clinopyroxene, Mg-Al spinel, anorthite, perovskite, and osbornite (TiN). In addition to these phases, the terminal particle, named 'Inti', also contains melilite. All of these phases, with the exception of osbornite, are common in refractory inclusions and are predicted to condense at high temperature from a gas of solar composition. Osbornite, though very rare, has also been found in meteoritic refractory inclusions, and could have formed in a region of the nebula where carbon became enriched relative to oxygen compared to solar composition. Compositions of Ti-pyroxene in Inti are similar, but not identical, to those of fassaite from Allende inclusions. Electron energy loss spectroscopy shows that Ti-rich pyroxene in Inti has Ti{sup 3+}/Ti{sup 4+} within the range of typical meteoritic fassaite, consistent with formation under reducing conditions comparable to those of a system of solar composition. Inti is {sup 16}O-rich, with {delta}{sup 18}O {approx} {delta}{sup 17}O {approx} 40{per_thousand}, like unaltered phases in refractory inclusions and refractory IDPs. With grain sizes, mineralogy, mineral chemistry, and an oxygen isotopic composition like those of refractory inclusions, we conclude that Inti is a refractory inclusion that formed in the inner solar nebula. Identification of a particle that formed in the inner Solar System among the comet samples demonstrates that there was transport of materials from the inner to the outer nebula, probably either in a bipolar outflow or by turbulence.

  13. Comparison of the oxidation state of Fe in comet 81P/Wild 2 and chondritic-porous interplanetary dust particles

    SciTech Connect (OSTI)

    Ogliore, Ryan C.; Butterworth, Anna L.; Fakra, Sirine C.; Gainsforth, Zack; Marcus, Matthew A.; Westphal, Andrew J.

    2010-07-16

    The fragile structure of chondritic-porous interplanetary dust particles (CP-IDPs) and their minimal parent-body alteration have led researchers to believe these particles originate in comets rather than asteroids where aqueous and thermal alterations have occurred. The solar elemental abundances and atmospheric entry speed of CP-IDPs also suggest a cometary origin. With the return of the Stardust samples from Jupiter-family comet 81P/Wild 2, this hypothesis can be tested. We have measured the Fe oxidation state of 15 CP-IDPs and 194 Stardust fragments using a synchrotron-based x-ray microprobe. We analyzed {approx}300 ng of Wild 2 material - three orders of magnitude more material than other analyses comparing Wild 2 and CP-IDPs. The Fe oxidation state of these two samples of material are > 2{sigma} different: the CP-IDPs are more oxidized than the Wild 2 grains. We conclude that comet Wild 2 contains material that formed at a lower oxygen fugacity than the parent-body, or parent bodies, of CP-IDPs. If all Jupiter-family comets are similar, they do not appear to be consistent with the origin of CP-IDPs. However, comets that formed from a different mix of nebular material and are more oxidized than Wild 2 could be the source of CP-IDPs.

  14. Hood River Steelhead Genetics Study; Relative Reproductive Success of Hatchery and Wild Steelhead in the Hood River, Final Report 2002-2003.

    SciTech Connect (OSTI)

    Blouin, Michael

    2003-05-01

    There is a considerable interest in using hatcheries to speed the recovery of wild populations. The Bonneville Power Administration (BPA), under the authority of the Northwest Power Planning Act, is currently funding several hatchery programs in the Columbia Basin as off-site mitigation for impacts to salmon and steelhead caused by the Columbia River federal hydropower system. One such project is located on the Hood River, an Oregon tributary of the Columbia. These hatchery programs cost the region millions of dollars. However, whether such programs actually improve the status of wild fish remains untested. The goal of this project was to evaluate the effectiveness of the Hood River hatchery program as required by the Northwest Power Planning Council Fish and Wildlife Program, by the Oregon Plan for Coastal Salmonids, by NMFS ESA Section 4(d) rulings, and by the Oregon Department of Fish and Wildlife (ODFW) Wild Fish Management Policy (OAR 635-07-525 through 529) and the ODFW Hatchery Fish Gene Resource Management Policy (OAR 635-07-540 through 541). The Hood River supports two populations of steelhead, a summer run and a winter run. They spawn only above the Powerdale Dam, which is a complete barrier to all salmonids. Since 1991 every adult passed above the dam has been measured, cataloged and sampled for scales. Therefore, we have a DNA sample from every adult steelhead that went over the dam to potentially spawn in the Hood River from 1991 to the present. Similar numbers of hatchery and wild fish have been passed above the dam during the last decade. During the 1990's 'old' domesticated hatchery stocks of each run (multiple generations in the hatchery, out-of-basin origin; hereafter H{sub old}) were phased out, and conservation hatchery programs were started for the purpose of supplementing the two wild populations (hereafter 'new' hatchery stocks, H{sub new}). These samples gave us the unprecedented ability to estimate, via microsatellite-based pedigree

  15. Draft Genome Sequences for Clostridium thermocellum Wild-Type Strain YS and Derived Cellulose Adhesion-Defective Mutant Strain AD2

    SciTech Connect (OSTI)

    Brown, Steven D; Lamed, Raphael; Morag, Ely; Borovok, Ilya; Shoham, Yuval; Klingeman, Dawn Marie; Johnson, Courtney M; Yang, Zamin; Land, Miriam L; Utturkar, Sagar M; Keller, Martin; Bayer, Edward A

    2012-01-01

    Clostridium thermocellum wild-type strain YS is an anaerobic, thermophilic, cellulolytic bacterium capable of directly converting cellulosic substrates into ethanol. Strain YS and a derived cellulose adhesion-defective mutant strain AD2 played pivotal roles in describing the original cellulosome concept. We present their draft genome sequences.

  16. Category:Energy Generation Facilities | Open Energy Information

    Open Energy Info (EERE)

    " title"Fossil Gulch Wind Park">Fossil Gulch Wind Park","title":"Fossil Gulch Wind Park","link":null,"lat":42.814261,"lon":-114.996665,"alt":0,"address":""...

  17. Genome sequence and analysis of a stress-tolerant, wild-derived strain of Saccharomyces cerevisiae used in biofuels research

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    McIlwain, Sean J.; Peris, Davis; Sardi, Maria; Moskvin, Oleg V.; Zhan, Fujie; Myers, Kevin S.; Riley, Nicholas M.; Buzzell, Alyssa; Parreiras, Lucas S.; Ong, Irene M.; et al

    2016-04-20

    The genome sequences of more than 100 strains of the yeast Saccharomyces cerevisiae have been published. Unfortunately, most of these genome assemblies contain dozens to hundreds of gaps at repetitive sequences, including transposable elements, tRNAs, and subtelomeric regions, which is where novel genes generally reside. Relatively few strains have been chosen for genome sequencing based on their biofuel production potential, leaving an additional knowledge gap. Here, we describe the nearly complete genome sequence of GLBRCY22-3 (Y22-3), a strain of S. cerevisiae derived from the stress-tolerant wild strain NRRL YB-210 and subsequently engineered for xylose metabolism. After benchmarking several genome assemblymore » approaches, we developed a pipeline to integrate Pacific Biosciences (PacBio) and Illumina sequencing data and achieved one of the highest quality genome assemblies for any S. cerevisiae strain. Specifically, the contig N50 is 693 kbp, and the sequences of most chromosomes, the mitochondrial genome, and the 2-micron plasmid are complete. Our annotation predicts 92 genes that are not present in the reference genome of the laboratory strain S288c, over 70% of which were expressed. We predicted functions for 43 of these genes, 28 of which were previously uncharacterized and unnamed. Remarkably, many of these genes are predicted to be involved in stress tolerance and carbon metabolism and are shared with a Brazilian bioethanol production strain, even though the strains differ dramatically at most genetic loci. Lastly, the Y22-3 genome sequence provides an exceptionally high-quality resource for basic and applied research in bioenergy and genetics.« less

  18. Recovering the Elemental Composition of Comet Wild 2 Dust in Five Stardust Impact Tracks and Terminal Particles in Aerogel

    SciTech Connect (OSTI)

    Ishii, H A; Brennan, S; Bradley, J P; Luening, K; Ignatyev, K; Pianetta, P

    2007-01-04

    The elemental (non-volatile) composition of five Stardust impact tracks and terminal particles left from capture of Comet 81P/Wild 2 dust were mapped in a synchrotron x-ray scanning microprobe with full fluorescence spectra at each pixel. Because aerogel includes background levels of several elements of interest, we employ a novel 'dual threshold' approach to discriminate against background contaminants: an upper threshold, above which a spectrum contains cometary material plus aerogel and a lower threshold below which it contains only aerogel. The difference between normalized cometary-plus-background and background-only spectra is attributable to cometary material. The few spectra in between are discarded since misallocation is detrimental: cometary material incorrectly placed in the background spectrum is later subtracted from the cometary spectrum, doubling the loss of reportable cometary material. This approach improves precision of composition quantification. We present the refined whole impact track and terminal particle elemental abundances for the five impact tracks. One track shows mass increases in Cr and Mn (1.4x), Cu, As and K (2x), Zn (4x) and total mass (13%) by dual thresholds compared to a single threshold. Major elements Fe and Ni are not significantly affected. The additional Cr arises from cometary material containing little Fe. We exclude Au intermixed with cometary material because it is found to be a localized surface contaminant carried by comet dust into an impact track. The dual threshold technique can be used in other situations where elements of interest in a small sample embedded in a matrix are also present in the matrix itself.

  19. AFM Imaging Reveals Topographic Diversity of Wild Type and Z Variant Polymers of Human α1-Proteinase Inhibitor

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gaczynska, Maria; Karpowicz, Przemyslaw; Stuart, Christine E.; Norton, Malgorzata G.; Teckman, Jeffrey H.; Marszal, Ewa; Osmulski, Pawel A.

    2016-03-23

    α1-Proteinase inhibitor (antitrypsin) is a canonical example of the serpin family member that binds and inhibits serine proteases. The natural metastability of serpins is crucial to carry out structural rearrangements necessary for biological activity. However, the enhanced metastability of the mutant Z variant of antitrypsin, in addition to folding defect, may substantially contribute to its polymerization, a process leading to incurable serpinopathy. The metastability also impedes structural studies on the polymers. There are no crystal structures of Z monomer or any kind of polymers larger than engineered wild type (WT) trimer. Our understanding of polymerization mechanisms is based on biochemicalmore » data using in vitro generated WT oligomers and molecular simulations. Here we applied atomic force microscopy (AFM) to compare topography of monomers, in vitro formed WT oligomers, and Z type polymers isolated from transgenic mouse liver. We found the AFM images of monomers closely resembled an antitrypsin outer shell modeled after the crystal structure. We confirmed that the Z variant demonstrated higher spontaneous propensity to dimerize than WT monomers. We also detected an unexpectedly broad range of different types of polymers with periodicity and topography depending on the applied method of polymerization. Short linear oligomers of unit arrangement similar to the Z polymers were especially abundant in heat-treated WT preparations. Long linear polymers were a prominent and unique component of liver extracts. However, the liver preparations contained also multiple types of oligomers of topographies undistinguishable from those found inWT samples polymerized with heat, low pH or guanidine hydrochloride treatments. In conclusion, we established that AFM is an excellent technique to assess morphological diversity of antitrypsin polymers, which is important for etiology of serpinopathies. These data also support previous, but controversial models of in vivo

  20. Optimization of scat detection methods for a social ungulate, the wild pig, and experimental evaluation of factors affecting detection of scat

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Keiter, David A.; Cunningham, Fred L.; Rhodes, Jr., Olin E.; Irwin, Brian J.; Beasley, James C.

    2016-05-25

    Collection of scat samples is common in wildlife research, particularly for genetic capture-mark-recapture applications. Due to high degradation rates of genetic material in scat, large numbers of samples must be collected to generate robust estimates. Optimization of sampling approaches to account for taxa-specific patterns of scat deposition is, therefore, necessary to ensure sufficient sample collection. While scat collection methods have been widely studied in carnivores, research to maximize scat collection and noninvasive sampling efficiency for social ungulates is lacking. Further, environmental factors or scat morphology may influence detection of scat by observers. We contrasted performance of novel radial search protocolsmore » with existing adaptive cluster sampling protocols to quantify differences in observed amounts of wild pig (Sus scrofa) scat. We also evaluated the effects of environmental (percentage of vegetative ground cover and occurrence of rain immediately prior to sampling) and scat characteristics (fecal pellet size and number) on the detectability of scat by observers. We found that 15- and 20-m radial search protocols resulted in greater numbers of scats encountered than the previously used adaptive cluster sampling approach across habitat types, and that fecal pellet size, number of fecal pellets, percent vegetative ground cover, and recent rain events were significant predictors of scat detection. Our results suggest that use of a fixed-width radial search protocol may increase the number of scats detected for wild pigs, or other social ungulates, allowing more robust estimation of population metrics using noninvasive genetic sampling methods. Further, as fecal pellet size affected scat detection, juvenile or smaller-sized animals may be less detectable than adult or large animals, which could introduce bias into abundance estimates. In conclusion, knowledge of relationships between environmental variables and scat detection may allow

  1. Transportation as a Means of Increasing Wild Juvenile Salmon Survival : Recovery Issues for Threatened and Endangered Snake River Salmon : Technical Report 4 of 11.

    SciTech Connect (OSTI)

    Park, Donn L.

    1993-06-01

    Smolt transportation on the Snake and Columbia Rivers has been under nearly continuous study for 25 years. Most controversy surrounds transport of spring/summer chinook, so most analyses and discussion are devoted to that species. Sockeye migrate at the same time as spring/summer chinook as do the earliest of the fall chinook. Therefore, action taken o spring/summer chinook will also affect sockeye and fall chinook. Many factors influenced transportation study results including population structure change -- the shift from nearly all wild fish to nearly all hatchery fish; new dams; the number of turbines at Snake River dams alone increased from 3 in 1968 to 24 by 1979; installation of juvenile fish pass facilities; and calamitous natural events such as the 1977 drought. All the above had negative effects on the survival of wild fish in general and on transport test results specifically, except that when smolts were transported from the upper dam their survival was not influenced by new or existing structures downstream from the transport site.

  2. Crystal Structures of Wild-type and Mutant Methicillin-resistant Staphylococcus aureus Dihydrofolate Reductase Reveal an Alternative Conformation of NADPH that may be Linked to Trimethoprim Resistance

    SciTech Connect (OSTI)

    Frey, K.; Liu, J; Lombardo, M; Bolstad, D; Wright, D; Anderson, A

    2009-01-01

    Both hospital- and community-acquired Staphylococcus aureus infections have become major health concerns in terms of morbidity, suffering and cost. Trimethoprim-sulfamethoxazole (TMP-SMZ) is an alternative treatment for methicillin-resistant S. aureus (MRSA) infections. However, TMP-resistant strains have arisen with point mutations in dihydrofolate reductase (DHFR), the target for TMP. A single point mutation, F98Y, has been shown biochemically to confer the majority of this resistance to TMP. Using a structure-based approach, we have designed a series of novel propargyl-linked DHFR inhibitors that are active against several trimethoprim-resistant enzymes. We screened this series against wild-type and mutant (F98Y) S. aureus DHFR and found that several are active against both enzymes and specifically that the meta-biphenyl class of these inhibitors is the most potent. In order to understand the structural basis of this potency, we determined eight high-resolution crystal structures: four each of the wild-type and mutant DHFR enzymes bound to various propargyl-linked DHFR inhibitors. In addition to explaining the structure-activity relationships, several of the structures reveal a novel conformation for the cofactor, NADPH. In this new conformation that is predominantly associated with the mutant enzyme, the nicotinamide ring is displaced from its conserved location and three water molecules complete a network of hydrogen bonds between the nicotinamide ring and the protein. In this new position, NADPH has reduced interactions with the inhibitor. An equilibrium between the two conformations of NADPH, implied by their occupancies in the eight crystal structures, is influenced both by the ligand and the F98Y mutation. The mutation induced equilibrium between two NADPH-binding conformations may contribute to decrease TMP binding and thus may be responsible for TMP resistance.

  3. Infrared Spectroscopy of Wild 2 Particle Hypervelocity Tracks in Stardust Aerogel: Evidence for the presence of Volatile Organics in Comet Dust

    SciTech Connect (OSTI)

    Bajt, S; Sandford, S A; Flynn, G J; Matrajt, G; Snead, C J; Westphal, A J; Bradley, J P

    2007-08-28

    Infrared spectroscopy maps of some tracks, made by cometary dust from 81P/Wild 2 impacting Stardust aerogel, reveal an interesting distribution of volatile organic material. Out of six examined tracks three show presence of volatile organic components possibly injected into the aerogel during particle impacts. When particle tracks contained excess volatile organic material, they were found to be -CH{sub 2}-rich. Off-normal particle tracks could indicate impacts by lower velocity particles that could have bounced off the Whipple shield, therefore carry off some contamination from it. However, this theory is not supported by data that show excess organic-rich material in normal and off-normal particle tracks. It is clear that the population of cometary particles impacting the Stardust aerogel collectors also include grains that contained little or none of this volatile organic component. This observation is consistent with the highly heterogeneous nature of the collected grains, as seen by a multitude of other analytical techniques. We propose that at least some of the volatile organic material might be of cometary origin based on supporting data shown in this paper. However, we also acknowledge the presence of carbon (primarily as -CH{sub 3}) in the original aerogel, which complicates interpretation of these results.

  4. Metabolic Engineering of Light and Dark Biochemical Pathways in Wild-Type and Mutant Strains of Synechocystis PCC 6803 for Maximal, 24-Hour Production of Hydrogen Gas

    SciTech Connect (OSTI)

    Ely, Roger L.; Chaplen, Frank W.R.

    2014-03-11

    This project used the cyanobacterial species Synechocystis PCC 6803 to pursue two lines of inquiry, with each line addressing one of the two main factors affecting hydrogen (H2) production in Synechocystis PCC 6803: NADPH availability and O2 sensitivity. H2 production in Synechocystis PCC 6803 requires a very high NADPH:NADP+ ratio, that is, the NADP pool must be highly reduced, which can be problematic because several metabolic pathways potentially can act to raise or lower NADPH levels. Also, though the [NiFe]-hydrogenase in PCC 6803 is constitutively expressed, it is reversibly inactivated at very low O2 concentrations. Largely because of this O2 sensitivity and the requirement for high NADPH levels, a major portion of overall H2 production occurs under anoxic conditions in the dark, supported by breakdown of glycogen or other organic substrates accumulated during photosynthesis. Also, other factors, such as N or S limitation, pH changes, presence of other substances, or deletion of particular respiratory components, can affect light or dark H2 production. Therefore, in the first line of inquiry, under a number of culture conditions with wild type (WT) Synechocystis PCC 6803 cells and a mutant with impaired type I NADPH-dehydrogenase (NDH-1) function, we used H2 production profiling and metabolic flux analysis, with and without specific inhibitors, to examine systematically the pathways involved in light and dark H2 production. Results from this work provided rational bases for metabolic engineering to maximize photobiological H2 production on a 24-hour basis. In the second line of inquiry, we used site-directed mutagenesis to create mutants with hydrogenase enzymes exhibiting greater O2 tolerance. The research addressed the following four tasks: 1. Evaluate the effects of various culture conditions (N, S, or P limitation; light/dark; pH; exogenous organic carbon) on H2 production profiles of WT cells and an NDH-1 mutant; 2. Conduct metabolic flux analyses for

  5. Evaluation of the 1998 Predictions of the Run-Timing of Wild Migrant Yearling Chinook and Water Quality at Multiple Locations on the Snake and Columbia Rivers using CRiSP/RealTime, 1998 Technical Report.

    SciTech Connect (OSTI)

    Beer, W. Nicholas; Hayes, Joshua A.; Shaw, Pamela

    1999-07-21

    Since 1988, wild salmon have been PIT-tagged through monitoring and research programs conducted by the Columbia River fisheries agencies and Tribes. Workers at the University of Washington have used detection data at Lower Granite Dam to generate predictions of arrival distributions for various stocks at the dam. The prediction tool is known as RealTime. In 1996, RealTime predictions were linked to a downstream migration model, CRiSP.1. The composite model, known as CRiSP/RealTime, predicts the arrival distribution and fraction transported at downriver locations.

  6. Interpretation of Wild 2 Dust Fine Structure: Comparison of Stardust Aluminium Foil Craters to the Three-Dimensional Shape of Experimental Impacts by Artificial Aggregate Particles and Meteorite Powders

    SciTech Connect (OSTI)

    Kearsley, A T; Burchell, M J; Price, M C; Graham, G A; Wozniakiewicz, P J; Cole, M J; Foster, N J; Teslich, N

    2009-12-10

    New experimental results show that Stardust crater morphology is consistent with interpretation of many larger Wild 2 dust grains being aggregates, albeit most of low porosity and therefore relatively high density. The majority of large Stardust grains (i.e. those carrying most of the cometary dust mass) probably had density of 2.4 g cm{sup -3} (similar to soda-lime glass used in earlier calibration experiments) or greater, and porosity of 25% or less, akin to consolidated carbonaceous chondrite meteorites, and much lower than the 80% suggested for fractal dust aggregates. Although better size calibration is required for interpretation of the very smallest impacting grains, we suggest that aggregates could have dense components dominated by {micro}m-scale and smaller sub-grains. If porosity of the Wild 2 nucleus is high, with similar bulk density to other comets, much of the pore-space may be at a scale of tens of micrometers, between coarser, denser grains. Successful demonstration of aggregate projectile impacts in the laboratory now opens the possibility of experiments to further constrain the conditions for creation of bulbous (Type C) tracks in aerogel, which we have observed in recent shots. We are also using mixed mineral aggregates to document differential survival of pristine composition and crystalline structure in diverse fine-grained components of aggregate cometary dust analogues, impacted onto both foil and aerogel under Stardust encounter conditions.

  7. Monitoring and Evaluation of Smolt Migration in the Columbia Basin : Volume XV : Evaluation of the 2007 Predictions of the Run-Timing of Wild and Hatchery-Reared Salmon and Steelhead Smolts to Rock Island, Lower Granite, McNary, John Day, and Bonneville Dams using Program RealTime.

    SciTech Connect (OSTI)

    Griswold, Jim; Townsend, Richard L.; Skalski, John R.

    2008-12-01

    Program RealTime provided monitoring and forecasting of the 2007 inseason outmigrations via the internet for 26 PIT-tagged stocks of wild ESU Chinook salmon and steelhead to Lower Granite and/or McNary dams, one PIT-tagged hatchery-reared ESU of sockeye salmon to Lower Granite Dam, one PIT-tagged wild stock of sockeye salmon to McNary Dam, and 20 passage-indexed runs-at-large, five each to Rock Island, McNary, John Day, and Bonneville dams. Nineteen stocks are of wild yearling Chinook salmon which were captured, PIT-tagged, and released at sites above Lower Granite Dam in 2007 and have at least one year's historical migration data previous to the 2007 migration. These stocks originate in 19 tributaries of the Salmon, Grande Ronde and Clearwater Rivers, all tributaries to the Snake River, and are subsequently detected through tag identification and monitored at Lower Granite Dam. Seven wild PIT-tagged runs-at-large of Snake or Upper Columbia River ESU salmon and steelhead were monitored at McNary Dam. Three wild PIT-tagged runs-at-large were monitored at Lower Granite Dam, consisting of the yearling and subyearling Chinook salmon and the steelhead runs. The hatchery-reared PIT-tagged sockeye salmon stock from Redfish Lake was monitored outmigrating through Lower Granite Dam. Passage-indexed stocks (stocks monitored by FPC passage indices) included combined wild and hatchery runs-at-large of subyearling and yearling Chinook, coho, and sockeye salmon, and steelhead forecasted to Rock Island, McNary, John Day, and Bonneville dams.

  8. Insulin-Like Growth Factor-Type 1 Receptor Inhibitor NVP-AEW541 Enhances Radiosensitivity of PTEN Wild-Type but Not PTEN-Deficient Human Prostate Cancer Cells

    SciTech Connect (OSTI)

    Isebaert, Sofie F.; Swinnen, Johannes V.; McBride, William H.; Haustermans, Karin M.

    2011-09-01

    Purpose: During the past decade, many clinical trials with both monoclonal antibodies and small molecules that target the insulin-like growth factor-type 1 receptor (IGF-1R) have been launched. Despite the important role of IGF-1R signaling in radioresistance, studies of such agents in combination with radiotherapy are lagging behind. Therefore, the aim of this study was to investigate the effect of the small molecule IGF-1R kinase inhibitor NVP-AEW541 on the intrinsic radioresistance of prostate cancer cells. Methods and Materials: The effect of NVP-AEW541 on cell proliferation, cell viability, IGF-1R signaling, radiosensitivity, cell cycle distribution, and double strand break repair was determined in three human prostate cancer cell lines (PC3, DU145, 22Rv1). Moreover, the importance of the PTEN pathway status was explored by means of transfection experiments with constitutively active Akt or inactive kinase-dead Akt. Results: NVP-AEW541 inhibited cell proliferation and decreased cell viability in a time-and dose-dependent manner in all three cell lines. Radiosensitization was observed in the PTEN wild-type cell lines DU145 and 22Rv1 but not in the PTEN-deficient PC3 cell line. NVP-AEW541-induced radiosensitization coincided with downregulation of phospho-Akt levels and high levels of residual double strand breaks. The importance of PTEN status in the radiosensitization effect was confirmed by transfection experiments with constitutively active Akt or inactive kinase-dead Akt. Conclusions: NVP-AEW541 enhances the effect of ionizing radiation in PTEN wild-type, but not in PTEN-deficient, prostate cancer cells. Proper patient selection based on the PTEN status of the tumor will be critical to the achievement of optimal results in clinical trials in which the combination of radiotherapy and this IGF-1R inhibitor is being explored.

  9. Monitoring and Evaluation of Smolt Migration in the Columbia Basin, Volume XIV; Evaluation of 2006 Prediction of the Run-Timing of Wild and Hatchery-Reared Salmon and Steelhead at Rock Island, Lower Granite, McNary, John Day and Bonneville Dams using Program Real Time, Technical Report 2006.

    SciTech Connect (OSTI)

    Griswold, Jim

    2007-01-01

    Program RealTime provided monitoring and forecasting of the 2006 inseason outmigrations via the internet for 32 PIT-tagged stocks of wild ESU chinook salmon and steelhead to Lower Granite and/or McNary dams, one PIT-tagged hatchery-reared ESU of sockeye salmon to Lower Granite Dam, and 20 passage-indexed runs-at-large, five each to Rock Island, McNary, John Day, and Bonneville Dams. Twenty-four stocks are of wild yearling chinook salmon which were captured, PIT-tagged, and released at sites above Lower Granite Dam in 2006, and have at least one year's historical migration data previous to the 2006 migration. These stocks originate in drainages of the Salmon, Grande Ronde and Clearwater Rivers, all tributaries to the Snake River, and are subsequently detected through the tag identification and monitored at Lower Granite Dam. In addition, seven wild PIT-tagged runs-at-large of Snake or Upper Columbia River ESU salmon and steelhead were monitored at McNary Dam. Three wild PIT-tagged runs-at-large were monitored at Lower Granite Dam, consisting of the yearling and subyearling chinook salmon and the steelhead trout runs. The hatchery-reared PIT-tagged sockeye salmon stock from Redfish Lake was monitored outmigrating through Lower Granite Dam. Passage-indexed stocks (stocks monitored by FPC passage indices) included combined wild and hatchery runs-at-large of subyearling and yearling chinook, coho, and sockeye salmon, and steelhead trout forecasted to Rock Island, McNary, John Day, and Bonneville Dams.

  10. Rocky Mountain Basins Produced Water Database

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Historical records for produced water data were collected from multiple sources, including Amoco, British Petroleum, Anadarko Petroleum Corporation, United States Geological Survey (USGS), Wyoming Oil and Gas Commission (WOGC), Denver Earth Resources Library (DERL), Bill Barrett Corporation, Stone Energy, and other operators. In addition, 86 new samples were collected during the summers of 2003 and 2004 from the following areas: Waltman-Cave Gulch, Pinedale, Tablerock and Wild Rose. Samples were tested for standard seven component "Stiff analyses", and strontium and oxygen isotopes. 16,035 analyses were winnowed to 8028 unique records for 3276 wells after a data screening process was completed. [Copied from the Readme document in the zipped file available at http://www.netl.doe.gov/technologies/oil-gas/Software/database.html] Save the Zipped file to your PC. When opened, it will contain four versions of the database: ACCESS, EXCEL, DBF, and CSV formats. The information consists of detailed water analyses from basins in the Rocky Mountain region.

  11. Wild and Scenic Rivers | Open Energy Information

    Open Energy Info (EERE)

    Scenic Rivers Jump to: navigation, search Retrieved from "http:en.openei.orgwindex.php?titleWildandScenicRivers&oldid612228" Feedback Contact needs updating Image...

  12. Wild Fire Computer Model Helps Firefighters

    ScienceCinema (OSTI)

    Canfield, Jesse

    2014-06-02

    A high-tech computer model called HIGRAD/FIRETEC, the cornerstone of a collaborative effort between U.S. Forest Service Rocky Mountain Research Station and Los Alamos National Laboratory, provides insights that are essential for front-line fire fighters. The science team is looking into levels of bark beetle-induced conditions that lead to drastic changes in fire behavior and how variable or erratic the behavior is likely to be.

  13. Proposed Wild Horse Pass Substation Option

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    LAK E C ITY, U T PAN EL 2 ELEC TR IC ITY D ISTR IBU TION AN D EN D -U SE: H OW D O W E MAN AGE C H ALLEN GES AN D OPPOR TU N ITIES? AP R I L 2 5 , 2 0 1 6 Gila River Indian ...

  14. Stop Codon Reassignment in the Wild

    SciTech Connect (OSTI)

    Ivanova, Natalia; Schwientek, Patrick; Tripp, H. James; Rinke, Christian; Pati, Amrita; Huntemann, Marcel; Visel, Axel; Woyke, Tanja; Kyrpides, Nikos; Rubin, Edward

    2014-03-21

    Since the discovery of the genetic code and protein translation mechanisms (1), a limited number of variations of the standard assignment between unique base triplets (codons) and their encoded amino acids and translational stop signals have been found in bacteria and phages (2-3). Given the apparent ubiquity of the canonical genetic code, the design of genomically recoded organisms with non-canonical codes has been suggested as a means to prevent horizontal gene transfer between laboratory and environmental organisms (4). It is also predicted that genomically recoded organisms are immune to infection by viruses, under the assumption that phages and their hosts must share a common genetic code (5). This paradigm is supported by the observation of increased resistance of genomically recoded bacteria to phages with a canonical code (4). Despite these assumptions and accompanying lines of evidence, it remains unclear whether differential and non-canonical codon usage represents an absolute barrier to phage infection and genetic exchange between organisms. Our knowledge of the diversity of genetic codes and their use by viruses and their hosts is primarily derived from the analysis of cultivated organisms. Advances in single-cell sequencing and metagenome assembly technologies have enabled the reconstruction of genomes of uncultivated bacterial and archaeal lineages (6). These initial findings suggest that large scale systematic studies of uncultivated microorganisms and viruses may reveal the extent and modes of divergence from the canonical genetic code operating in nature. To explore alternative genetic codes, we carried out a systematic analysis of stop codon reassignments from the canonical TAG amber, TGA opal, and TAA ochre codons in assembled metagenomes from environmental and host-associated samples, single-cell genomes of uncultivated bacteria and archaea, and a collection of phage sequences

  15. Manufacturing Demonstration Facilities Workshop Agenda, March...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Manufacturing Demonstration Facilities Workshop Marriott Springhill Suites O'Hare - ... mechanics of the Manufacturing Demonstration Facility (MDF) concept and the ...

  16. Remedial action plan and site design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado: Final report

    SciTech Connect (OSTI)

    Not Available

    1992-02-01

    This volume contains Appendix F, bid schedule and specifications for remedial action on three sites: Old Rifle processing site; New Rifle processing site and Estes Gulch disposal site.

  17. Remedial action plan and site design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado: Final report. Volume 3, Appendix F, Final design, specifications, and drawings

    SciTech Connect (OSTI)

    Not Available

    1992-02-01

    This volume contains Appendix F, bid schedule and specifications for remedial action on three sites: Old Rifle processing site; New Rifle processing site and Estes Gulch disposal site.

  18. Nevada Site Office News News Media Contact: For Immediate...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    GRITTY GULCH FIRE CONTAINED AT THE NEVADA NATIONAL SECURITY SITE North Las Vegas - Current information on the wildfire at the Nevada National Security Site (NNSS): NEW ...

  19. First Direct Evidence of Dirac Fermions in Graphite

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of finite effective mass and defect-induced localized states. Goodbye Silicon Valley, Hello Graphite Gulch? Why are scientists suddenly interested in graphite? It is, after all,...

  20. Floodplain and Wetlands Assessment for Construction of a Second...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Floodplain and Wetlands Assessment for Construction of a Second Full Service Access Road over Lena Gulch Floodplain and Wetlands Assessment for Construction of a Second Full ...

  1. 1 Advisory, Consultative, Deliberative, Draft SANDIA REPORT

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... for Wind (CREW) Program Update 11 5 Data Sources The transition of the CREW Program to ... Windy GulchAll Turbine Group Potential Energy Production Turbine XXX SUSPENDED ...

  2. Taming the Wild World of Management, Performance and Communication - 13459

    SciTech Connect (OSTI)

    Ford, Laurie

    2013-07-01

    Management has evolved a long way from its original meaning of 'governing a horse'. The industrial revolution fostered 'scientific management'; 1930's Hawthorne studies discovered that people's social interactions could alter productivity; and the dawn of the computer age in the post-war 1950's brought general systems theory into management thinking. Today, mobile wireless connectivity aims to transform ever-changing networks of players, mandates, and markets into something that can be 'managed'. So why is there no clear and simple recipe for how to practice management? We talk about financial management, safety management, and operations management, but surely the 'management' part of those endeavors will share the same set of practices. Instead, we are still arguing for 'management' to include everything from developing people to negotiating contracts. A manager's job may include many things, but one of them, the job of management, needs to be nailed down. Three standard practices for managing in a network are developed: (a) support the dialogues that connect people vital to accomplishing a goal or objective; (b) develop and sustain the scoreboards that serve as a road-map to reach the goal; and (c) control the feedback to 'govern the horse'. These three practices are useful for more than reaching goals, as they also support coordinating across boundaries and running productive meetings. The dialogues for productive relationships, scoreboards for goals and deliverables, and feedback for performance together constitute a recipe for managing in a networked world. (authors)

  3. Dosage compensation can buffer copy-number variation in wild...

    Office of Scientific and Technical Information (OSTI)

    Publisher: eLife Sciences Publications, Ltd. Sponsoring Org: USDOE Country of Publication: United States Language: English Word Cloud More Like This Free Publicly Accessible Full ...

  4. EV Everywhere Challenge Battery Workshop

    Broader source: Energy.gov [DOE]

    Backsplash for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  5. EV Everywhere Grand Challenge- Battery Status and Cost Reduction Prospects

    Office of Energy Efficiency and Renewable Energy (EERE)

    Presentation given by technology manager David Howell at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  6. EV Everywhere EV Everywhere Grand Challenge- Electric Drive (Power Electronics and Electric Machines) Workshop Agenda

    Broader source: Energy.gov [DOE]

    Agenda for the EV Everywhere Grand Challenge - Electric Drive Workshop on July 24, 2012 at the Doubletree O'Hare, Chicago, IL

  7. EV Everywhere Batteries Workshop- Beyond Lithium Ion Breakout Session Report

    Office of Energy Efficiency and Renewable Energy (EERE)

    Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  8. Department of Energy Seeks Public Comment on Designation of Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    MT Holiday Inn 22 North Last Chance Gulch Helena, MT 59601 November 1, 2005 Boise, ID Harrison Plaza Suite Hotel 409 S. Cole Road Boise, ID 83709 November 1, 2005 Sacramento, CA ...

  9. ARM - Logistics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Silver Gulch 2195 Old Steese Highway (great beer selection) Reservations: 907.452.2739 Turtle Club 10-Mile Old Steese Highway (great prime rib) Reservations: 907.457.3883 Zach's ...

  10. EV Everywhere Grand Challenge - Battery Status and Cost Reduction...

    Broader source: Energy.gov (indexed) [DOE]

    Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. 5howellb.pdf (1.24 MB) More Documents & Publications PHEV Battery Cost Assessment ...

  11. David Turner! NERSC User Services Group NERSC Data Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Data Management --- 1 --- September 10, 2013 Overview * File S ystem R eview, D ata S haring, D ata T ransfer - David T urner * GlobusOnline D emo - Shreyas C holia * HPSS - Lisa G ...

  12. EV Everywhere Workshop: Electric Motors and Critical Materials Breakout Group Report

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  13. A Look at MEMS Applications | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    this video on www.youtube.com, or enable JavaScript if it is disabled in your browser. hare This Article Click to email this to a friend (Opens in new window) Share on Facebook...

  14. ARM - Publications: Science Team Meeting Documents

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    on the TOGA-COARE Bulk Algorithm for Computing the Air-Sea Fluxes Fairall, C.W., and Hare, J.E., National Oceanic and Atmospheric Administration- Environmental Technology...

  15. EV Everywhere Battery Workshop: Preliminary Target-Setting Framework

    Broader source: Energy.gov [DOE]

    Presentation given by Vehicle Technologies Office analyst Jacob Ward at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL.

  16. EV Everywhere Electric Drive Workshop: Preliminary Target-Setting Framework

    Broader source: Energy.gov [DOE]

    Presentation given by Vehicle Technologies Office analyst Jake Ward at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  17. EV Everywhere Grand Challenge Introduction for Electric Drive Workshop

    Broader source: Energy.gov [DOE]

    Presentation given by EERE Assistant Secretary David Danielson at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  18. This list does not imply DOE endorsement of the individuals or...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Jeff jeff.hare@cascadeenergy.com 801-770-4332 UT Hash IV, Robert bhash@airsystemsales.com 615-373-0039 TN Hunter, Bill bhunter@aircleantech.com 206-860-4930 WA J Jackson, ...

  19. Electric Motors and Critical Materials

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  20. Traction Drive Systems Breakout

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  1. EV Everywhere Workshop: Traction Drive Systems Breakout Group Report

    Broader source: Energy.gov [DOE]

    Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL.

  2. Shreyas Cholia! Data and Analytics Services, NERSC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Design Patterns in Web Gateways for Scientific Data --- 1 --- Joint F acili+es U ser F ... --- 2 --- Overview * Mo+va+on * PaDerns - Web D ata S haring - End---to---End F rameworks ...

  3. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    U.S. Energy Information Administration (EIA) Indexed Site

    BIG PINEY TIP TOP BIR D CANYON SWAN FONTEN ELL E LABARGE HOGSBACK CHIMNEY BUT TE BIG PINEY AREA TIP TOP UNI T LINCOLN ROAD BLU E FOREST DEER HILL FOGART Y CREEK GREEN RIVER BEND ...

  4. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    U.S. Energy Information Administration (EIA) Indexed Site

    BIG PINEY TIP TOP BIR D CANYON SWAN FONTEN ELL E LABARGE HOGSBACK CHIMNEY BUT TE BIG PINEY AREA TIP TOP UNI T LINCOLN ROAD BLU E FOREST SWAN DEER HILL FOGART Y CREEK GREEN RIVER ...

  5. Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Nayak, Jaladhi; Gastonguay, Adam J.; Talipov, Marat R.; Vakeel, Padmanabhan; Span, Elise A.; Kalous, Kelsey S.; Kutty, Raman G.; Jensen, Davin R.; Pokkuluri, Phani Raj; Sem, Daniel S.; et al

    2014-12-18

    Background: The mitogen-activated protein kinases (MAPKs) pathway is critical for cellular signaling, and proteins such as phosphatases that regulate this pathway are important for normal tissue development. Based on our previous work on dual specificity phosphatase-5 (DUSP5), and its role in embryonic vascular development and disease, we hypothesized that mutations in DUSP5 will affect its function. Results: In this study, we tested this hypothesis by generating full-length glutathione-S-transferase-tagged DUSP5 and serine 147 proline mutant (S147P) proteins from bacteria. Light scattering analysis, circular dichroism, enzymatic assays and molecular modeling approaches have been performed to extensively characterize the protein form and function.more » We demonstrate that both proteins are active and, interestingly, the S147P protein is hypoactive as compared to the DUSP5 WT protein in two distinct biochemical substrate assays. Furthermore, due to the novel positioning of the S147P mutation, we utilize computational modeling to reconstruct full-length DUSP5 and S147P to predict a possible mechanism for the reduced activity of S147P. Conclusion: Taken together, this is the first evidence of the generation and characterization of an active, full-length, mutant DUSP5 protein which will facilitate future structure-function and drug development-based studies.« less

  6. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    U.S. Energy Information Administration (EIA) Indexed Site

    Authors: Sam Limerick (1), Lucy Luo (1), Gary Long (2), David Morehouse (2), Jack Perrin (1), Steve Jackson (1) and Robert King (2) (1) Z, Inc., (2) Energy Information ...

  7. Selective progressive response of soil microbial community to wild oat roots

    SciTech Connect (OSTI)

    DeAngelis, K.M.; Brodie, E.L.; DeSantis, T.Z.; Andersen, G.L.; Lindow, S.E.; Firestone, M.K.

    2008-10-01

    Roots moving through soil enact physical and chemical changes that differentiate rhizosphere from bulk soil, and the effects of these changes on soil microorganisms have long been a topic of interest. Use of a high-density 16S rRNA microarray (PhyloChip) for bacterial and archaeal community analysis has allowed definition of the populations that respond to the root within the complex grassland soil community; this research accompanies previously reported compositional changes, including increases in chitinase and protease specific activity, cell numbers and quorum sensing signal. PhyloChip results showed a significant change in 7% of the total rhizosphere microbial community (147 of 1917 taxa); the 7% response value was confirmed by16S rRNA T-RFLP analysis. This PhyloChip-defined dynamic subset was comprised of taxa in 17 of the 44 phyla detected in all soil samples. Expected rhizosphere-competent phyla, such as Proteobacteria and Firmicutes, were well represented, as were less-well-documented rhizosphere colonizers including Actinobacteria, Verrucomicrobia and Nitrospira. Richness of Bacteroidetes and Actinobacteria decreased in soil near the root tip compared to bulk soil, but then increased in older root zones. Quantitative PCR revealed {beta}-Proteobacteria and Actinobacteria present at about 10{sup 8} copies of 16S rRNA genes g{sup -1} soil, with Nitrospira having about 10{sup 5} copies g{sup -1} soil. This report demonstrates that changes in a relatively small subset of the soil microbial community are sufficient to produce substantial changes in function in progressively more mature rhizosphere zones.

  8. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    this tangle, the picture that emerged is of cometary particles containing primarily silicate materials formed within the Solar System, including some grains born in the high...

  9. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    them contributed to many of the findings that are shedding new light on how the Solar System was formed. The Stardust results show that the mixing of materials throughout the...

  10. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Particles from Comet 81PWild 2 Viewed by ALS Microscopes Print NASA's 200-million, seven-year-long Stardust mission returned to Earth thousands of tiny particles snagged from the...

  11. Comet 81P/Wild 2 Under a Microscope (Journal Article) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Comet 81PWild 2 Under a Microscope Citation Details In-Document Search Title: Comet ... Publication Date: 2007-01-16 OSTI Identifier: 897746 Report Number(s): ...

  12. Particles from Comet 81P/Wild 2 Viewed by ALS Microscopes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Stardust mission returned to Earth thousands of tiny particles snagged from the coma of comet 81PWild 2. Four ALS beamlines and the researchers using them were among the...

  13. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Reserve Class No 2001 gas reserves 0.1 - 10 MMCF 10.1 - 100 MMCF 100.1 - 1,000 MMCF 1,000.1 - 10,000 MMCF 10,000.1 - 100,000 MMCF > 100,000 MMCF Basin Outline ID The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). The boundaries are not informed by subsurface

  14. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    U.S. Energy Information Administration (EIA) Indexed Site

    (1) and Robert King (2) (1) Z, Inc., (2) Energy Information Administration BIG PINEY TIP ... LINCOLN ROAD BLU E FOREST DEER HILL FOGART Y CREEK GREEN RIVER BEND DRY PINEY SWAN S ...

  15. BR UFF BIG PINEY WILD ROSE BLU E GAP BR UFF UNIT WAMSUT TER

    U.S. Energy Information Administration (EIA) Indexed Site

    (1) and Robert King (2) (1) Z, Inc., (2) Energy Information Administration BIG PINEY TIP ... BLU E FOREST SWAN DEER HILL FOGART Y CREEK GREEN RIVER BEND DRY PINEY SWAN S HOGSBACK AREA ...

  16. Ankit Bhagatwala! NERSC User Services Group! New User Training!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ankit Bhagatwala! NERSC User Services Group! ! New User Training! March 21, 2016 Computing Environment Node Types * Login nodes - Shared w ith o ther u sers - Code c ompila3on, j ob p repara3on a nd s ubmission * Compute n odes - Not s hared ( except s hared q ueue o n C ori) * Service nodes - File s ystem a ccess ( lustre), d ata m ovement ( HPSS), network c onnec3ons t o o utside w orld, e tc. - Not a ccessed d irectly --- 2 --- Login Node Configuration * Edison - Twelve n odes * 16 c ores, 2

  17. Radiation-induced DNA damage and the relative biological effectiveness of 18F-FDG in wild-type mice

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taylor, Kristina; Lemon, Jennifer A.; Boreham, Douglas R.

    2014-05-28

    Clinically, the most commonly used positron emission tomography (PET) radiotracer is the glucose analog 2-[18F] fluoro-2-deoxy-d-glucose (18F-FDG), however little research has been conducted on the biological effects of 18F-FDG injections. The induction and repair of DNA damage and the relative biological effectiveness (RBE) of radiation from 18F-FDG relative to 662 keV γ-rays were investigated. The study also assessed whether low-dose radiation exposure from 18F-FDG was capable of inducing an adaptive response. DNA damage to the bone marrow erythroblast population was measured using micronucleus formation and lymphocyte γH2A.X levels. To test the RBE of 18F-FDG, mice were injected with a rangemore » of activities of 18F-FDG (0–14.80 MBq) or irradiated with Cs-137 γ-rays (0–100 mGy). The adaptive response was investigated 24 h after the 18F-FDG injection by 1 Gy in vivo challenge doses for micronucleated reticulocyte (MN-RET) formation or 1, 2 and 4 Gy in vitro challenges doses for γH2A.X formation. A significant increase in MN-RET formation above controls occurred following injection activities of 3.70, 7.40 or 14.80 MBq (P < 0.001) which correspond to bone marrow doses of ~35, 75 and 150 mGy, respectively. Per unit dose, the Cs-137 radiation exposure induced significantly more damage than the 18F-FDG injections (RBE = 0.79 ± 0.04). A 20% reduction in γH2A.X fluorescence was observed in mice injected with a prior adapting low dose of 14.80 MBq 18F-FDG relative to controls (P < 0.019). A 0.74 MBq 18F-FDG injection, which gives mice a dose approximately equal to a typical human PET scan, did not cause a significant increase in DNA damage nor did it generate an adaptive response. Typical 18F-FDG injection activities used in small animal imaging (14.80 MBq) resulted in a decrease in DNA damage, as measured by γH2A.X formation, below spontaneous levels observed in control mice. Lastly, the 18F-FDG RBE was <1.0, indicating that the mixed radiation quality and/or low dose rate from PET scans is less damaging than equivalent doses of gamma radiation.« less

  18. BPA-2013-01256-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    M1C1-Ti SSQcJTES, RC. AttojrneyN at.Law RECEIVED BY tWA FOIA OFFICE TillS DATE - 1143 DUE DATE: OF COUNS 6L 0014 5. KATES bATneonow4b. WA RuTh P HARING IL&Tri5w fri. HC'RDCZIcO Ws...

  19. Fujifilm_NERSC_StorageOutlook.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - Workload c an s aturate s ystem a nd c ause i ssues f or s hared u sers - Beam l ine o r e nd s taon w ork s chedule d rives b andwidth n eeds --- 6 --- Addressing large...

  20. Oct_2015_IM_Response_to_Public_Comments.xlsx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    e nrollment a nd p rior t o a ny S EM a ctivity, u tilities w ill r eceive a " user n ame" a nd "password" t o a ccess t he s ecure o nline E SI S ecure F ile S hare (...

  1. EV Everywhere Framing Workshop - Report Out & Lessons Learned | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy - Report Out & Lessons Learned EV Everywhere Framing Workshop - Report Out & Lessons Learned Presentation given at the EV Everywhere Grand Challenge : Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. 2_davis_ed.pdf (589.87 KB) More Documents & Publications EV Everywhere Framing Workshop

  2. EV Everywhere Framing Workshop Report Out & Lessons Learned | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Report Out & Lessons Learned EV Everywhere Framing Workshop Report Out & Lessons Learned Presentation given at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. 3_davis_b.pdf (588.37 KB) More Documents & Publications EV Everywhere Framing Workshop -

  3. Richard Gerber! NERSC User Services NUG Teleconference

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... 4 --- Fair Share Scheduling Experiment on Edison * What a re c urrent u sage g oals? - DARPA M ission P artners: 2 5% - Each D OE O ffice g ets a s hare o f t he r emaining 7 5% b ...

  4. Nevada Site Office News News Media Contact: For Immediate Release:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3, 2011 702-630-0120 (afterhours) 2:39:15 PM (PST) 702-683-0601 (afterhours) ***UPDATE*** CREWS FIGHTING WILDFIRE AT THE NEVADA NATIONAL SECURITY SITE North Las Vegas - Current information on the wildfire at the Nevada National Security Site (NNSS): NEW INFORMATION * Fire named Gritty Gulch Fire * Approximately 29 acres burned * Bureau of Land Management is conducting slurry air drops to surround the fire * The fire does not appear to be advancing PREVIOUS INFORMATION * Located in a remote area

  5. Utilization of ash from municipal solid waste combustion

    SciTech Connect (OSTI)

    Jones, C.; Hahn, J.; Magee, B.; Yuen, N.; Sandefur, K.; Tom, J.; Yap, C.

    1999-09-01

    This ash study investigated the beneficial use of municipal waste combustion combined ash from the H-POWER facility in Oahu. These uses were grouped into intermediate cover for final closure of the Waipahu landfill, daily cover at the Waimanalo Gulch Landfill, and partial replacement for aggregate in asphalt for road paving. All proposed uses examine combined fly and bottom ash from a modern waste-to-energy facility that meets requirements of the Clean Air Act Amendments for Maximum Achievable Control Technology.

  6. Remedial action plan and site design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado

    SciTech Connect (OSTI)

    Not Available

    1992-02-01

    This appendix assesses the present conditions and data gathered about the two inactive uranium mill tailings sites near Rifle, Colorado, and the designated disposal site six miles north of Rifle in the area of Estes Gulch. It consolidates available engineering, radiological, geotechnical, hydrological, meteorological, and other information pertinent to the design of the Remedial Action Plan (RAP). The data characterize conditions at the mill, tailings, and disposal site so that the Remedial Action Contractor (RAC) may complete final designs for the remedial actions.

  7. Remedial action plan and site design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado. Volume 2, Appendices D and E: Final report

    SciTech Connect (OSTI)

    Not Available

    1992-02-01

    This appendix assesses the present conditions and data gathered about the two inactive uranium mill tailings sites near Rifle, Colorado, and the designated disposal site six miles north of Rifle in the area of Estes Gulch. It consolidates available engineering, radiological, geotechnical, hydrological, meteorological, and other information pertinent to the design of the Remedial Action Plan (RAP). The data characterize conditions at the mill, tailings, and disposal site so that the Remedial Action Contractor (RAC) may complete final designs for the remedial actions.

  8. Significant Increase in Hydrogen Photoproduction Rates and Yields by Wild-Type Algae is Detected at High Photobioreactor Gas Phase Volume (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2012-07-01

    This NREL Hydrogen and Fuel Cell Technical Highlight describes how hydrogen photoproduction activity in algal cultures can be improved dramatically by increasing the gas-phase to liquid-phase volume ratio of the photobioreactor. NREL, in partnership with subcontractors from the Institute of Basic Biological Problems in Pushchino, Russia, demonstrated that the hydrogen photoproduction rate in algal cultures always decreases exponentially with increasing hydrogen partial pressure above the culture. The inhibitory effect of high hydrogen concentrations in the photobioreactor gas phase on hydrogen photoproduction by algae is significant and comparable to the effect observed with some anaerobic bacteria.

  9. Radiation-induced DNA damage and the relative biological effectiveness of 18F-FDG in wild-type mice

    SciTech Connect (OSTI)

    Taylor, Kristina; Lemon, Jennifer A.; Boreham, Douglas R.

    2014-05-28

    Clinically, the most commonly used positron emission tomography (PET) radiotracer is the glucose analog 2-[18F] fluoro-2-deoxy-d-glucose (18F-FDG), however little research has been conducted on the biological effects of 18F-FDG injections. The induction and repair of DNA damage and the relative biological effectiveness (RBE) of radiation from 18F-FDG relative to 662 keV γ-rays were investigated. The study also assessed whether low-dose radiation exposure from 18F-FDG was capable of inducing an adaptive response. DNA damage to the bone marrow erythroblast population was measured using micronucleus formation and lymphocyte γH2A.X levels. To test the RBE of 18F-FDG, mice were injected with a range of activities of 18F-FDG (0–14.80 MBq) or irradiated with Cs-137 γ-rays (0–100 mGy). The adaptive response was investigated 24 h after the 18F-FDG injection by 1 Gy in vivo challenge doses for micronucleated reticulocyte (MN-RET) formation or 1, 2 and 4 Gy in vitro challenges doses for γH2A.X formation. A significant increase in MN-RET formation above controls occurred following injection activities of 3.70, 7.40 or 14.80 MBq (P < 0.001) which correspond to bone marrow doses of ~35, 75 and 150 mGy, respectively. Per unit dose, the Cs-137 radiation exposure induced significantly more damage than the 18F-FDG injections (RBE = 0.79 ± 0.04). A 20% reduction in γH2A.X fluorescence was observed in mice injected with a prior adapting low dose of 14.80 MBq 18F-FDG relative to controls (P < 0.019). A 0.74 MBq 18F-FDG injection, which gives mice a dose approximately equal to a typical human PET scan, did not cause a significant increase in DNA damage nor did it generate an adaptive response. Typical 18F-FDG injection activities used in small animal imaging (14.80 MBq) resulted in a decrease in DNA damage, as measured by γH2A.X formation, below spontaneous levels observed in control mice. Lastly, the 18F-FDG RBE was <1.0, indicating that the mixed radiation quality and/or low dose rate from PET scans is less damaging than equivalent doses of gamma radiation.

  10. NERSC User Services Group Hybrid MPI/ OpenMP Programming

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    He! NERSC User Services Group Hybrid MPI/ OpenMP Programming --- 1 --- NUG 2 013, F ebruary 1 5, 2 013 Outline * Architecture T rend * Brief R eview o f O penMP B asics * Benefits o f H ybrid M PI/OpenMP * Hybrid M PI/OpenMP P rogramming M odel * Hybrid M PI/OpenMP I ssues * Compile a nd R un h ybrid M PI/OpenMP a t N ERSC Common Architectures * Shared M emory A rchitecture - Mul'ple C PUs s hare g lobal m emory, c ould h ave l ocal c ache - Uniform M emory A ccess ( UMA) - Typical S hared M

  11. Yun (Helen) He, NERSC!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NERSC! Mar 23, 2015 OpenMP Basics and MPI/OpenMP Scaling --- 1 --- Outline * Introduc)ons t o O penMP * Features i n O penMP 3 .1 * What's n ew i n O penMP 4 .0 * Adding O penMP t o y our c ode u sing C ray R eveal * Hybrid M PI/OpenMP S caling --- 2 --- Introductions to OpenMP --- 3 --- Common Architectures * Shared M emory A rchitecture - Mul)ple C PUs s hare g lobal m emory, c ould h ave l ocal c ache - Uniform M emory A ccess ( UMA) - Typical S hared M emory P rogramming M odel: O penMP,

  12. Lisa Gerhardt! NERSC User Services Group! NUG Training!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    February 23, 2015 Data Management at NERSC Where Do I Put My Data? --- 2 --- * Overview o f N ERSC fi le s ystems - Local v s. G lobal - Permanent v s. P urged - Personal v s. S hared * HPSS A rchive S ystem - What i s i t a nd h ow t o u se i t * Data S haring NERSC File Systems --- 3 --- The compute and storage systems 2015 Produc=on C lusters Carver, P DSF, J GI, MatComp, Planck /global/ scratch 4 PB /project 5 PB /home 250 TB 70 P B s tored, 2 40 P B capacity, 4 0 y ears o f community d ata

  13. CCM_NUG20120202.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hopper c ompute n odes r un _ ______ A. L ight v ersion o f O pera9ng S ystem B. S tandard ( full) L inux O pera9ng S ystem * Hopper c ompute n odes d on't s upport _ _____ A. D ynamic s hared l ibraries B. s sh C. B oth A a nd B Hopper q uiz * Hopper c ompute n odes r un _ ______ A. L ight v ersion o f O pera9ng S ystem B. S tandard ( full) L inux O pera9ng S ystem * Hopper c ompute n odes d on't s upport _ _____ A. D ynamic s hared l ibraries B. s sh C. B oth A a nd B Hopper q uiz A B This t

  14. Blue Gene/Q: Sequoia and Mira | Argonne Leadership Computing Facility

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Blue Gene/Q: Sequoia and Mira Authors: Bill Allcock, Anna Maria Bailey, Ray Bair, Charles Bacon, Ramesh Balakrishnan, Adam Bertsch, Barna Bihari, Brian Carnes, Dong Chen, George Chiu, Richard Coffey, Susan Coghlan, Paul Coteus, Kim Cupps, Erik W. Draeger, Thomas W. Fox, Larry Fried, Mark Gary, Jim Glosli, Thomas Gooding, John Gunnels, John Gyllenhaal, Jeff Hammond, Ruud Haring, Philip Heidelberger, Mark Hereld, Todd Inglett, K.H. Kim, Kalyan Kumaran, Steve Langer, Amith Mamidala, Rose McCallen,

  15. EVMS Training Snippet: 1.1 DOE Order 413.3B EVM Requirements | Department

    Office of Environmental Management (EM)

    Report | Department of Energy Batteries Workshop - Beyond Lithium Ion Breakout Session Report EV Everywhere Batteries Workshop - Beyond Lithium Ion Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. report_out-beyond_lithium_ion_b.pdf (138.93 KB) More Documents & Publications EV Everywhere Batteries Workshop - Next Generation Lithium Ion Batteries Breakout Session

  16. EV Everywhere Batteries Workshop - Materials Processing and Manufacturing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakout Session Report | Department of Energy Materials Processing and Manufacturing Breakout Session Report EV Everywhere Batteries Workshop - Materials Processing and Manufacturing Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. report_out-manufacturing_b.pdf (117.4 KB) More Documents & Publications EV Everywhere Batteries Workshop - Next Generation Lithium Ion

  17. EV Everywhere Batteries Workshop - Next Generation Lithium Ion Batteries

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Breakout Session Report | Department of Energy Next Generation Lithium Ion Batteries Breakout Session Report EV Everywhere Batteries Workshop - Next Generation Lithium Ion Batteries Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. report_out-next-generation_li-ion_b.pdf (136.48 KB) More Documents & Publications EV Everywhere Batteries Workshop - Beyond Lithium Ion

  18. EV Everywhere Batteries Workshop - Pack Design and Optimization Breakout

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Session Report | Department of Energy Pack Design and Optimization Breakout Session Report EV Everywhere Batteries Workshop - Pack Design and Optimization Breakout Session Report Breakout session presentation for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. report_out-pack_design_b.pdf (141.92 KB) More Documents & Publications EV Everywhere Batteries Workshop - Beyond Lithium Ion Breakout Session Report EV Everywhere

  19. EV Everywhere Battery Workshop Introduction | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Workshop Introduction EV Everywhere Battery Workshop Introduction Presentation given at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. 1_sandalow_b.pdf (3.21 MB) More Documents & Publications EV Everywhere Framing Workshop Overview EV Everywhere Consumer Acceptance and Charging Infrastructure Workshop Introduction Battery and Electric Drive Awardee List from American Recovery and Reinvestment Act funding

  20. EV Everywhere Battery Workshop: Setting the Stage for the EV Everywhere

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Grand Challenge | Department of Energy Setting the Stage for the EV Everywhere Grand Challenge EV Everywhere Battery Workshop: Setting the Stage for the EV Everywhere Grand Challenge Presentation given at the EV Everywhere Grand Challenge: Battery Workshop by EERE Assistant Secretary David Danielson on July 26, 2012 at the Doubletree O'Hare, Chicago, Illinois. 2 Danielson EV Everywhere Battery presentation [Read-Only].pdf (408.26 KB) More Documents & Publications EV Everywhere Grand

  1. EV Everywhere Grand Challenge - Battery Workshop Agenda | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Agenda EV Everywhere Grand Challenge - Battery Workshop Agenda Agenda for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree O'Hare, Chicago, IL. agenda_b.pdf (196.97 KB) More Documents & Publications EV Everywhere Grand Challenge - Charge to the Breakout Groups EV Everywhere Grand Challenge Introduction for Electric Drive Workshop EV Everywhere Grand Challenge Kick-Off

  2. EV Everywhere Grand Challenge - Battery Workshop attendees list |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy attendees list EV Everywhere Grand Challenge - Battery Workshop attendees list Attendance list for the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. companies_in_attendance_b.pdf (149.45 KB) More Documents & Publications EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop EV Everywhere Grand Challenge Overview EV Everywhere Grand Challenge Introduction for

  3. EV Everywhere Grand Challenge - Charge to the Breakout Groups | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Charge to the Breakout Groups EV Everywhere Grand Challenge - Charge to the Breakout Groups Presentation given at the EV Everywhere Grand Challenge: Battery Workshop on July 26, 2012 held at the Doubletree OHare, Chicago, IL. 7_howell_b.pdf (796.65 KB) More Documents & Publications EV Everywhere - Charge to Breakout Sessions EV Everywhere Grand Challenge - Battery Workshop Agenda EV Everywhere EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric

  4. EV Everywhere Grand Challenge - Electric Drive (Power Electronics and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electric Machines) Workshop | Department of Energy - Electric Drive (Power Electronics and Electric Machines) Workshop EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop List of companies in attendance at the Electric Drive Workshop held on July 24, 2012 at the Doubletree O'Hare, Chicago, IL companies_in_attendance_ed.pdf (145.65 KB) More Documents & Publications EV Everywhere Grand Challenge Introduction for Electric Drive Workshop EV

  5. EV Everywhere Workshop: Power Electronics and Thermal Management Breakout

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Session Report | Department of Energy Power Electronics and Thermal Management Breakout Session Report EV Everywhere Workshop: Power Electronics and Thermal Management Breakout Session Report Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. 9b_traction_drive_systems_ed.pdf (122.88 KB) More Documents & Publications EV Everywhere Batteries Workshop -

  6. Research Highlight

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Modification of the Atmospheric Boundary Layer by a Small Island: Observations from Nauru Submitter: Long, C. N., NOAA Global Monitoring Division/CIRES Area of Research: Cloud Distributions/Characterizations Working Group(s): Radiative Processes Journal Reference: Matthews, S., J. M. Hacker, J. Cole, J. Hare, C. N. Long, and R. M. Reynolds, (2007): Modification of the atmospheric boundary layer by a small island: observations from Nauru, MWR, Vol. 135, No. 3, pages 891–905. Figure 1.

  7. Electric Vehicle Safety Training for Emergency Responders | Department of

    Broader source: Energy.gov (indexed) [DOE]

    Presentation given at the EV Everywhere Grand Challenge … Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. 7b_electric_motors-and_critical_materials_ed.pdf (154.22 KB) More Documents & Publications EV Everywhere Workshop: Power Electronics and Thermal Management Breakout Session Report EV Everywhere Workshop: Electric Motors and Critical Materials Breakout Group Report EV Everywhere - Charge to Breakout Sessions

  8. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Surface Energy Budget Measurements from Nauru99 C. W. Fairall, J. E. Hare, A. A. Grachev, and A. B. White National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The National Oceanic and Atmospheric Administration (NOAA) research vessel (R/V) Ronald H. Brown conducted a series of measurements in transit to and in the vicinity of the U.S. Department of Energy (DOE) Cloud and Radiation Testbed (CART) site on Nauru in June-July 1999 as part

  9. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Examination of Island Effects on Near-Surface Bulk Meteorology and Air-Sea Fluxes from the Nauru99 Field Program C. W. Fairall and M. J. Post National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado J. E. Hare, A. B. White, and A. A. Grachev Cooperative Institute for Research in Environmental Sciences University of Colorado and National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The

  10. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nauru99 Ship and Buoy Intercomparison J. E. Hare and A. A. Grachev Cooperative Institute for Research in Environmental Sciences, University of Colorado National Oceanic and Atmospheric Administration, Environmental Technology Laboratory Boulder, Colorado C. W. Fairall and M. J. Post National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado R. M. Reynolds and S. Smith Brookhaven National Laboratory Brookhaven, New York H. Ishida Kobe University of

  11. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Turbulent Surface Flux Measurements from Nauru99 C. W. Fairall, J. E. Hare, and A. A. Grachev National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The National Oceanic and Atmospheric Administration (NOAA) Research Vessel (R/V) Ronald H. Brown conducted a series of measurements in transit to and in the vicinity of the U.S. Department of Energy (DOE) Cloud and Radiation Testbed (CART) site on Nauru in June-July 1999 as part of a joint

  12. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Observation of 4-5 Day Meridional Wind and Surface Stress Oscillations During Nauru99 A. A. Grachev and J. E. Hare University of Colorado Cooperative Institute for Research in Environmental Sciences National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado C. W. Fairall National Oceanic and Atmospheric Administration Environmental Technology Laboratory Boulder, Colorado Introduction The existence of the easterly wave disturbances in the Tropical

  13. QA Corporate Board Meeting - June 2010 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    June 2010 QA Corporate Board Meeting - June 2010 7th EM Quality Assurance Corporate Board Meeting Meeting Location: Marriott Chicago O'Hare, 8535 West Higgins Road, Chicago, IL 60631 Room: TBD Draft Agenda for June 9, 2010 Documents Available for Download Meeting and Presentation Materials (6.16 MB) Meeting Minutes (173.99 KB) More Documents & Publications 2010 Quality Assurance Improvement Project Plan 2012 Quality Assurance Improvement Project Plan QA Corporate Board Meeting - September

  14. Fermilab | Visit Fermilab | Transportation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Transportation Transportation to and from Chicago O'Hare Airport or Midway Airport is available by limousine, taxi or car rental. Transportation to and from the Geneva local commuter Metra train station on the Union Pacific West line is available by taxi or Pace Call-n-Ride. Car rental All of the usual rental companies (such as Hertz, Avis, Budget and National) are located at the airports. Limousine service Reservations for limousine service should be made in advance when possible. West Suburban

  15. Nevada Site Office News News Media Contact: For Immediate Release:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4, 2011 11:56:36 AM (PST) ***UPDATE*** GRITTY GULCH FIRE CONTAINED AT THE NEVADA NATIONAL SECURITY SITE North Las Vegas - Current information on the wildfire at the Nevada National Security Site (NNSS): NEW INFORMATION * The fire is 100 percent contained * Bureau of Land Management completed 24 slurry air drops during the duration of the fire * Approximately 29 acres burned PREVIOUS INFORMATION * Located in a remote area (Area 19) of the 1,360-square-mile site * Fire most likely started from

  16. Microsoft Word - GUN 2005 CR.doc

    Office of Legacy Management (LM)

    Gunnison, Colorado Page 8-1 8.0 Gunnison, Colorado, Disposal Site 8.1 Compliance Summary The Gunnison Disposal Site, inspected on June 21, 2005, was in excellent condition. Six perimeter sign and the entrance sign were missing and bullets had damaged several others. All former erosion areas were stable. Areas reseeded in 2004 along the former Chance Gulch haul road require further monitoring, and therefore, the BLM right-of-way permit is still active. Revegetation of reseeded areas on Tenderfoot

  17. Table 2. Ten largest plants by generation capacity, 2014

    U.S. Energy Information Administration (EIA) Indexed Site

    Idaho" ,"Plant","Primary energy source","Operating company","Net summer capacity (MW)" 1,"Brownlee","Hydroelectric","Idaho Power Co",744 2,"Dworshak","Hydroelectric","USACE Northwestern Division",400 3,"Langley Gulch Power Plant","Natural gas","Idaho Power Co",299.7 4,"Evander Andrews Power Complex","Natural gas","Idaho Power

  18. Remedial action plan and site conceptual design for stabilization of the inactive uranium mill tailings sites at Rifle, Colorado. Appendix D, Final report

    SciTech Connect (OSTI)

    1992-02-01

    This appendix assesses the present conditions and data gathered about the two designated inactive uranium mill tailings sites near Rifle, Colorado, and the proposed disposal site six miles north of Rifle in the area of Estes Gulch. It consolidates available engineering, radiological, geotechnical, hydrological, meteorological, and other information pertinent to the design of the Remedial Action Plan (RAP). The data characterize conditions at the mill, tailings, and disposal site so that the Remedial Action Contractor (RAC) may complete final designs for the remedial actions.

  19. Significance of cyclic Pennsylvanian-Permian coral/algal buildups Snaky Canyon

    SciTech Connect (OSTI)

    Canter, K.L. ); Isaacson, P.E. )

    1991-02-01

    Five cyclic algal, hydrozoan, and coral buildups occur within a thick sequence of Pennsylvanian-Permian (Virgilian through Wolfcampain) carbonates in south-central Idaho. The Juniper Gulch Member of the Snaky Canyon Formation, as described by Skipp and coworkers, is approximately 600 m thick and contains four depositional facies, including: (1) open circulation outer( ) platform, (2) hydrozoan and phylloid algal mound-dominated carbonate buildup, (3) backmound, restricted platform/lagoon, and (4) restricted inner platform facies. Several microlithofacies, including lime mud-rich bafflestone, diversely fossiliferous packstone and grainstone, bryozoan lime floatstone, and phylloid algal and hydrozoan (Palaeoaplysina) lime bindstone are described within the phylloid algal mounds. Successional faunal assemblage stages are recognized within the buildups. Colonial rugose corals comprise a stabilization stage. When the algal communities of the diversification stage reached wave base, because of their rapid upward growth, cross-bedded oolitic grainstone and occasional cross-bedded dolomite shoals developed. Supratidal to high intertidal platform sedimentation is represented by dolomitic Palaeoaplysina bindstone, algal mat bindstone, and vuggy dolomite. Five vertical sequences of buildup development, each terminate by intertidal, supratidal, or erosional events, are seen in the Juniper Gulch Member in the North Howe stratigraphic section of the southern Lost River Range. The carbonate platform was constructed within a depositional basin that includes an eroded highland to the west, and a mixed siliciclastic-carbonate inner platform with craton uplifts to the east.

  20. E

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    E nergy S ciences N etwork: Overview a nd I mpact NERSC U sers G roup 2 016 Berkeley, CA March 2 1, 2 016 Kate P etersen M ace Energy S ciences N etwork ( ESnet) Science E ngagement E ngineer Lawrence B erkeley N aNonal L aboratory Overview Impact 2 ESnet is a dedicated m ission n etwork e ngineered to accelerate a broad range of science outcomes. We d o t his b y o ffering u nique c apabiliNes, a nd opNmizing t he n etwork f or d ata a cquisiNon, d ata placement, d ata s haring, d ata m

  1. David Turner! NERSC User Services Group!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computing Environment Node Types * Login nodes - Shared - Job p repara,on a nd s ubmission * MOM n odes - Shared - Where b atch s cript e xecutes * Parallel j ob l auncher - Hopper/Edison: a prun - Carver: m pirun * Compute n odes - Not s hared * Except s erial q ueue --- 2 --- Login Nodes * Edison - Six n odes * 16 c ores, 2 .0GHz I ntel S andy B ridge * 512GB * Hopper - Eight n odes * 16 c ores, 2 .4GHz A MD O pteron - Four n odes * 32 c ores, 2 .0GHz A MD O pteron - 128GB * Carver - Four n

  2. David Turner! NERSC User Services Group! NUG New User Training!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computing Environment Node Types * Login nodes - Shared - Job p repara,on a nd s ubmission * MOM n odes - Shared - Where b atch s cript e xecutes * Parallel j ob l auncher - Hopper/Edison: a prun - Carver: m pirun * Compute n odes - Not s hared * Except s erial q ueue --- 2 --- Login Nodes * Edison - Twelve n odes * 16 c ores, 2 .0 G Hz I ntel S andy B ridge, 5 12 G B * Hopper - Eight n odes * 16 c ores, 2 .4 G Hz A MD O pteron, 1 28GB - Four n odes * 32 c ores, 2 .0 G Hz A MD O pteron, 1 28GB *

  3. David Turner! NERSC User Services Group! New User Training!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computing Environment Node Types * Login nodes - Shared - Job p repara,on a nd s ubmission * MOM n odes - Shared - Where b atch s cript e xecutes * Parallel j ob l auncher - Hopper/Edison: a prun - Carver: m pirun * Compute n odes - Not s hared * Except s erial q ueue --- 2 --- Login Nodes * Edison - Twelve n odes * 16 c ores, 2 .0 G Hz I ntel S andy B ridge, 5 12 G B * Hopper - Eight n odes * 16 c ores, 2 .4 G Hz A MD O pteron, 1 28GB - Four n odes * 32 c ores, 2 .0 G Hz A MD O pteron, 1 28GB *

  4. Scott French! NERSC User Services Group! New User Training!

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    French! NERSC User Services Group! ! New User Training! August 13, 2015 Computing Environment Node Types * Login nodes - Shared w ith o ther u sers - Code c ompila3on, j ob p repara3on a nd s ubmission * MOM n odes ( Cray m achines) - Shared w ith o ther u sers - Where b atch s cript e xecutes - Will g o a way w hen w e t ransi3on t o S LURM * Compute n odes - Not s hared ( except s erial q ueues) --- 2 --- Login Node Configuration * Edison - Twelve n odes * 16 c ores, 2 .0 G Hz I ntel S andy B

  5. MLM-462 Contract Number AT-33-l-GtiN-53 MOUND LABORATCRY Operatsd By

    Office of Legacy Management (LM)

    This Doeumr;t Consists of 116 ?zges This is COPY of i.3 k MLM-462 Contract Number AT-33-l-GtiN-53 MOUND LABORATCRY Operatsd By MONSAmOC~ALGoMPANY MIAMISBURG, OHIO REPORT NO. 3 OF STIEUING CaW'M'B FOR DISPUSAL CF UNITS 111 AND IV (Completion Report for Disposal of UrLt IV, Runnymeade Road sr,d Dixon live. Dayton, Ohlo) . Date: April 17, 1950 Prepared By: -36L254- F. L. Halbach Chairnan., St33ricg Gcicmittes 1ssue.d: DISTRIHJTION copy 1. - Dr. Carroll A. Hochwalt copy 2. - Dr. M. M. Haring copy 3.

  6. Electric Drive Transportation Association Conference | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Presentation given at the EV Everywhere Grand Challenge - Electric Drive (Power Electronics and Electric Machines) Workshop on July 24, 2012 held at the Doubletree O'Hare, Chicago, IL. 4_rogers_ed.pdf (1.3 MB) More Documents & Publications Power Electronics and Thermal Management Breakout Session Advanced Power Electronics and Electric Motors (APEEM) R&D Program Overview Advanced Power Electronics and Electric Motors (APEEM) R&D Program Overview

    Test Drive 1 of 5 Test Drive Deputy

  7. 04-Environment-Turner.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computing Environment Node Types * Login nodes - Shared - Job p repara,on a nd s ubmission * MOM n odes - Shared - Where b atch s cript e xecutes * Parallel j ob l auncher - Hopper/Edison: a prun - Carver: m pirun * Compute n odes - Not s hared * Except s erial q ueue --- 2 --- Login Nodes * Edison - Twelve n odes * 16 c ores, 2 .0GHz I ntel S andy B ridge * 512GB * Hopper - Eight n odes * 16 c ores, 2 .4GHz A MD O pteron - Four nodes * 32 c ores, 2 .0GHz A MD O pteron - 128GB * Carver - Four

  8. 06-DataManagement-Gerhardt.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    New User Training! July 15, 2014 Data Management at NERSC Where Do I Put My Data? --- 2 --- * Overview o f N ERSC fi le s ystems - Local v s. G lobal - Permanent v s. P urged * HPSS A rchive S ystem - What i s i t a nd h ow t o u se i t * Data S haring NERSC File Systems --- 3 --- The compute and storage systems 2014 Produc<on C lusters Carver, P DSF, J GI, MatComp, Planck /global/ scratch 4 PB /project 5 PB /home 250 TB 65 PB stored, 240 PB capacity, 4 0 y ears o f community d ata HPSS 16 x

  9. 08-2-SharingDataWeb-Cholia.pptx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NERSC User Training, Aug 2015! Sharing Data at NERSC Sharing Data Internally --- 2 --- The /project directory * Your p roject w ill h ave a s hared p roject d irectory at /global/project/projectdirs/<dirname> * Shared group permissions for your project repo * h=ps://www.nersc.gov/users/storage---and---file--- systems/sharing---data/ --- 3 --- Give/take * Give a fi le t o a u ser: - give --- u < recipient---username> < file---or---directory> - This s ends i s i nto a h olding a

  10. CMN

    Office of Legacy Management (LM)

    sl CMN - t%1UB SECRET Copy 15 MI.-396 L a SCIOTO LABORATORY MARION, OHIO ~S5_ Ct-Jt4 0 This Document Consists of 57 Pages This is Copy / o rf 23A ~S~~g.^a^~T CAMLM-396 !eo T /xY/ 'INITIALS i Contract Number AT-33-1-CEN-53 RHTG # I 1- BOX ry scIOTr LABORATORY C Operated By MONSANTO CHEMICAL COMPANY MARION, OHIO Er M. M. Haring ,u Laboratory Director rq - -- r - Er OUTLINE FOR "COLD STAND-BY" OPERATION OF SCIOTO LABORATORY * (Limited Operation and Maintenance) CAUTION This document

  11. Microsoft Word - Document11

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    S cheinker Can s tudying i nfinite d imensional s pace h elp u s i mprove h ealthcare? Abstract: Could a hotel with a guest in each room give a new guest her own room without asking a nyone t o l eave o r s hare r ooms? C ould a s urgeon w ith a p erfect s calpel c ut a pea into 5 pieces and reassemble them into a sphere the size of the sun? Could the dough lying in a pizza pan be pushed out of the middle to form a ring of crust without being ripped? In our world, the answer to each of these

  12. Microsoft Word - appxa

    Office of Legacy Management (LM)

    GS05: Woman Creek at West Fenceline A.1.4 GS08: South Walnut Creek at Pond B-5 Outlet A.1.5 GS10: South Walnut Creek at B-1 Bypass A.1.6 GS11: North Walnut Creek at Pond A-4 Outlet A.1.7 GS12: North Walnut Creek at Pond A-3 Outlet A.1.8 GS13: North Walnut Creek Above Pond A-1 A.1.9 GS31: Woman Creek at Pond C-2 Outlet A.1.10 GS33: No Name Gulch at Walnut Creek A.1.11 GS51: Ditch South of Former 903 Pad A.1.12 GS59: Woman Creek Upstream of Antelope Springs Confluence A.1.13 B5INFLOW: South Walnut

  13. Microwave digestion techniques in the sequential extraction of calcium, iron, chromium, manganese, lead, and zinc in sediments

    SciTech Connect (OSTI)

    Mahan, K.I.; Foderaro, T.A.; Garza, T.L.; Martinez, R.M.; Maroney, G.A.; Trivisonno, M.R.; Willging, E.M.

    1987-04-01

    The sequential extraction scheme of Tessier partitions metals in sediments into exchangeable, carbonate bound iron-manganese oxide bound, organic bound, and residual binding fractions. Extraction rate experiments using conventional and microwave heating showed that microwave heating produces results comparable to the conventional procedure. Sequential microwave extraction procedures were established from the results of the extraction rate experiments. Recoveries of total metals from NBS SRM 1645 ranged from 76% to 120% for the conventional procedure and 62% to 120% for the microwave procedure. Recoveries of total metals using the microwave and conventional techniques were reasonably comparable except for iron (62% by microwave vs. 76% by conventional). Substitution of an aqua regia/HF extraction for total/residual metals results in essentially complete recovery of metals. Precision obtained from 31 replicate samples of the California Gulch, Colorado, sediment yielded about an average 11% relative standard deviation excluding the exchangeable fraction which was more variable.

  14. Gas-and water-saturated conditions in the Piceance Basin, Western Colorado: Implications for fractured reservoir detection in a gas-centered coal basin

    SciTech Connect (OSTI)

    Hoak, T.E.; Decker, A.D.

    1995-10-01

    Mesaverde Group reservoirs in the Piceance Basin, Western Colorado contain a large reservoir base. Attempts to exploit this resource base are stymied by low permeability reservoir conditions. The presence of abundant natural fracture systems throughout this basin, however, does permit economic production. Substantial production is associated with fractured reservoirs in Divide Creek, Piceance Creek, Wolf Creek, White River Dome, Plateau, Shire Gulch, Grand Valley, Parachute and Rulison fields. Successful Piceance Basin gas production requires detailed information about fracture networks and subsurface gas and water distribution in an overall gas-centered basin geometry. Assessment of these three parameters requires an integrated basin analysis incorporating conventional subsurface geology, seismic data, remote sensing imagery analysis, and an analysis of regional tectonics. To delineate the gas-centered basin geometry in the Piceance Basin, a regional cross-section spanning the basin was constructed using hydrocarbon and gamma radiation logs. The resultant hybrid logs were used for stratigraphic correlations in addition to outlining the trans-basin gas-saturated conditions. The magnitude of both pressure gradients (paludal and marine intervals) is greater than can be generated by a hydrodynamic model. To investigate the relationships between structure and production, detailed mapping of the basin (top of the Iles Formation) was used to define subtle subsurface structures that control fractured reservoir development. The most productive fields in the basin possess fractured reservoirs. Detailed studies in the Grand Valley-Parachute-Rulison and Shire Gulch-Plateau fields indicate that zones of maximum structural flexure on kilometer-scale structural features are directly related to areas of enhanced production.

  15. Richard Gerber! NERSC Senior Science Advisor! User Services Group Lead

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    User Services Group Lead NUG Webinar January 2014 --- 1 --- January 9 , 2 014 Agenda * Edison C onfigura6on f or A Y 2 014 * Alloca6on Y ear R ollover I ssues * Project D irectories p er P roject * NUG 2 014 U ser G roup M ee6ng * Automa6c I /O P rofiling f or Y our C ode * Give / T ake U 6lity f or S haring F iles * NUG Q ueue C ommiRee U pdate * User Survey Needs Your Input * Open D iscussion --- 2 --- Edison Update --- 3 --- Jeff B roughton NERSC D eputy f or O pera6ons Systems D epartment H

  16. H

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    H ybrid M PI/OpenMP, UPC, a nd C AF a t N ERSC Yun ( Helen) H e a nd W oo---Sun Y ang NERSC U ser G roup M eeFng February 2 , 2 012 2 Outline * Architecture T rend * Benefits o f H ybrid M PI/OpenMP * Hybrid M PI/OpenMP P rogramming M odel * Hybrid M PI/OpenMP I ssues * Compile a nd R un h ybrid M PI/OpenMP * Using U PC a nd C AF o n H opper 3 Common A rchitectures * Shared M emory A rchitecture - MulFple C PUs s hare g lobal m emory, c ould h ave l ocal c ache - Uniform M emory A ccess ( UMA) -

  17. Shreyas Cholia! Data and Analytics Services, NERSC How To Share

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    How To Share Data With Your Collaborators --- 1 --- Joint F acili+es U ser F orum o n D ata---Intensive Compu+ng, O akland, J une 1 8, 2 014 Overview * Introduc+on * Data S haring o ver H TTP * APIs * High P erformance D ata A ccess * Data M anagement S ystems * We'll t ry t o d o a f ew l ive d emos a long t he w ay --- 2 --- Introduction --- 3 --- Data Islands * Data i s s +ll h eavily s iloed a nd i naccessible. * Data s its o n a m achine s omewhere, a nd y ou g ive people l ocal a ccounts t

  18. Title 16 U.S.C. 1278(a) Restrictions on Water Resources Projects...

    Open Energy Info (EERE)

    under the Federal Power Act on or directly affecting any river which is designated as a component of the national wild and scenic rivers system under the Wild and Scenic Rivers...

  19. Advocate- Issue 47- July 2012

    Broader source: Energy.gov [DOE]

    Here are some of the topics from this issue: Wild West Tour, EM SSAB Chairs’ Meeting, and Reservation Updates.

  20. Stock Assessment of Columbia River Anadromous Salmonids : Final Report, Volume II, Steelhead Stock Summaries, Stock Transfer Guidelines, Information Needs.

    SciTech Connect (OSTI)

    Howell, Philip J.

    1985-07-01

    This report presents brief descriptions of wild and hatchery-raised steelhead trout stocks in the Columbia River Basin. (ACR)

  1. Late glacial aridity in southern Rocky Mountains

    SciTech Connect (OSTI)

    Davis, O.K.; Pitblado, B.L.

    1995-09-01

    While the slopes of the present-day Colorado Rocky Mountains are characterized by large stands of subalpine and montane conifers, the Rockies of the late glacial looked dramatically different. Specifically, pollen records suggest that during the late glacial, Artemisia and Gramineae predominated throughout the mountains of Colorado. At some point between 11,000 and 10,000 B.P., however, both Artemisia and grasses underwent a dramatic decline, which can be identified in virtually every pollen diagram produced for Colorado mountain sites, including Como Lake (Sangre de Cristo Mountains), Copley Lake and Splains; Gulch (near Crested Butte), Molas Lake (San Juan Mountains), and Redrock Lake (Boulder County). Moreover, the same pattern seems to hold for pollen spectra derived for areas adjacent to Colorado, including at sites in the Chuska Mountains of New Mexico and in eastern Wyoming. The implications of this consistent finding are compelling. The closest modem analogues to the Artemisia- and Gramineae-dominated late-glacial Colorado Rockies are found in the relatively arid northern Great Basin, which suggests that annual precipitation was much lower in the late-glacial southern Rocky Mountains than it was throughout the Holocene.

  2. Delineation of Piceance Basin basement structures using multiple source data: Implications for fractured reservoir exploration

    SciTech Connect (OSTI)

    Hoak, T.E.; Klawitter, A.L.

    1995-10-01

    Fractured production trends in Piceance Basin Cretaceous-age Mesaverde Group gas reservoirs are controlled by subsurface structures. Because many of the subsurface structures are controlled by basement fault trends, a new interpretation of basement structure was performed using an integrated interpretation of Landsat Thematic Mapper (TM), side-looking airborne radar (SLAR), high altitude, false color aerial photography, gas and water production data, high-resolution aeromagnetic data, subsurface geologic information, and surficial fracture maps. This new interpretation demonstrates the importance of basement structures on the nucleation and development of overlying structures and associated natural fractures in the hydrocarbon-bearing section. Grand Valley, Parachute, Rulison, Plateau, Shire Gulch, White River Dome, Divide Creek and Wolf Creek fields all produce gas from fractured tight gas sand and coal reservoirs within the Mesaverde Group. Tectonic fracturing involving basement structures is responsible for development of permeability allowing economic production from the reservoirs. In this context, the significance of detecting natural fractures using the intergrated fracture detection technique is critical to developing tight gas resources. Integration of data from widely-available, relatively inexpensive sources such as high-resolution aeromagnetics, remote sensing imagery analysis and regional geologic syntheses provide diagnostic data sets to incorporate into an overall methodology for targeting fractured reservoirs. The ultimate application of this methodology is the development and calibration of a potent exploration tool to predict subsurface fractured reservoirs, and target areas for exploration drilling, and infill and step-out development programs.

  3. Using Experience to Train the Next Generation of Workers | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Using Experience to Train the Next Generation of Workers Using Experience to Train the Next Generation of Workers July 5, 2011 - 1:46pm Addthis Bob Wilds working at a winding machine. | Photo Courtesy of Waukesha Electric Systems Bob Wilds working at a winding machine. | Photo Courtesy of Waukesha Electric Systems Lindsey Geisler Lindsey Geisler Public Affairs Specialist, Office of Public Affairs Bob Wilds has worked at Waukesha Electric Systems for the last 12 years as a coil winder.

  4. ckm4195.tmp

    Office of Scientific and Technical Information (OSTI)

    ... Threshold effects concentration Threshold effect level U.S. Fish and Wildlife Service . ... Protection Agency (EPA), U.S. Fish and Wild- life Service (USFWS), ...

  5. Derivative-free optimization for parameter estimation in computational...

    Office of Scientific and Technical Information (OSTI)

    nuclear physics Citation Details In-Document Search Title: Derivative-free optimization for parameter estimation in computational nuclear physics Authors: Wild, S ; ...

  6. Once nearly extinct, Idaho sockeye regaining fitness advantage

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in the wild once more. A newly published analysis by the Idaho Department of Fish and Game and the Northwest Fisheries Science Center shows endangered Snake River...

  7. Beasley Lab | Savannah River Ecology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Aging an anesthetized bobcat Wildlife studies in the Chernobyl exclusion zone Chernobyl wolf Amanda attaching satellite transmitter to a vulture Anesthetizing a wild pig ...

  8. SREL Reprint #3246

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    We artificially incubated wood duck (Aix sponsa) eggs at three temperature regimes (low, 35.0; ... ducklings from each temperature treatment were placed with wild foster mothers ...

  9. SREL Reprint #3203

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science, Amherst, Massachusetts, USA 4Bulmer and Associates, Auckland, New Zealand Abstract: In 1957, Troughton described the wild dog of New Guinea, naming it Canis hallstromi. ...

  10. RAPID/Roadmap/12-ID-a | Open Energy Information

    Open Energy Info (EERE)

    Contact Us State Biological Resource Considerations (12-ID-a) The Idaho Department of Fish & Game preserves wildlife against any direct take, including wild animals, birds, and...

  11. Structure of the DUF2233 Domain in Bacteria and the Stuttering...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    importance of most of these conserved residues as judged by comparison with the activity of wild-type UCE and its influence on trafficking to the Golgi. Similar...

  12. Fifteenmile Basin Habitat Enhancement Project: Annual Report...

    Office of Scientific and Technical Information (OSTI)

    wild winter steelhead in the Fifteenmile Creek Basin under the Columbia River Basin Fish and Wildlife Program. The project is funded by through the Bonneville Power...

  13. Appliance Rebates: Frequently Asked Questions | Department of...

    Energy Savers [EERE]

    Spikes Former Communicator at DOE's National Renewable Energy Laboratory The appliance rebate program has been wildly successful in many states. So successful, in fact, that people...

  14. RAPID/Roadmap/20-FD-b | Open Energy Information

    Open Energy Info (EERE)

    on one of the following: Indian lands; Wilderness areas; Wild and scenic rivers; Wetlands; Units of the National Park System, National Refuges, or National Fish Hatcheries;...

  15. RAPID/Roadmap/7-FD-f | Open Energy Information

    Open Energy Info (EERE)

    on one of the following: Indian lands; Wilderness areas; Wild and scenic rivers; Wetlands; Units of the National Park System, National Refuges, or National Fish Hatcheries;...

  16. Technical Sessions S. Lovejoy, A. Davis, Y. Tessier Physics Department

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    C. Gautier, D. Lavallee Earth Space Research Group University of California Santa Barbara, ... and wild statistics which require an infinite number of exponents to characterize. ...

  17. Social behavior and the microbiome (Journal Article) | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Social behavior and the microbiome Prev Next Title: Social behavior and the microbiome Social interactions influence the communities of microbes that live in wild baboons. ...

  18. The ADVANCE project: Insights and achievments

    SciTech Connect (OSTI)

    1996-12-31

    ADVANCE [Advanced Driver and Vehicle Advisory Navigation ConcEpt] was a public/private partnership conceived and developed by four founding parties. The founding parties include the Federal Highway Administration (FHWA), the Illinois Department of Transportation (IDOT), the University of Illinois at Chicago and Northwestern University operating together under the auspices of the Illinois Universities Transportation Research Consortium (IUTRC), and Motorola, Inc. The major responsibilities of each party are fully described in the Project agreement. Subsequently, these four were joined on the Steering Committee by the American Automobile Association (AAA). This unique blending of public sector, private sector and university interests, augmented by more than two dozen other private sector participants, provided a strong set of resources for ADVANCE. The ADVANCE test area covered over 300 square miles including portions of the City of Chicago and 40 northwest suburban communities. The Project encompasses the high growth areas adjacent to O`Hare International Airport, the Schaumburg/Hoffman Estates office and retail complexes, and the Lake-Cook Road development corridor. It also includes major sports and entertainment complexes such as the Arlington International Racecourse and the Rosemont Horizon. The population in the area is more than 750,000. The Insights and Perspectives Compendium is intended to provide useful information to project managers, system developers, and system integrators of future similar ITS implementations. It is intended for those that are technically interested in the ADVANCE Project and have a basic understanding of the project.

  19. The ADVANCE project: Formal evaluation of the targeted deployment. Volume 3

    SciTech Connect (OSTI)

    1997-01-01

    ADVANCE [Advanced Driver and Vehicle Advisory Navigation ConcEpt] was a public/private partnership conceived and developed by four founding parties. The founding parties include the Federal Highway Administration (FHWA), the Illinois Department of Transportation (IDOT), the University of Illinois at Chicago and Northwestern University operating together under the auspices of the Illinois Universities Transportation Research Consortium (IUTRC), and Motorola, Inc. The major responsibilities of each party are fully described in the Project agreement. Subsequently, these four were joined on the Steering Committee by the American Automobile Association (AAA). This unique blending of public sector, private sector and university interests, augmented by more than two dozen other private sector participants, provided a strong set of resources for ADVANCE. The ADVANCE test area covered over 300 square miles including portions of the City of Chicago and 40 northwest suburban communities. The Project encompasses the high growth areas adjacent to O`Hare International Airport, the Schaumbura/Hoffman Estates office and retail complexes, and the Lake-Cook Road development corridor. It also includes major sports and entertainment complexes such as the Arlington International Racecourse and the Rosemont Horizon. The population in the area is more than 750,000. This volume provides a summary of the insights and achievements made as a result of this field test, and selected appendices containing more detailed information.

  20. Retractable barrier strip

    DOE Patents [OSTI]

    Marts, Donna J.; Barker, Stacey G.; Wowczuk, Andrew; Vellenoweth, Thomas E.

    2002-01-01

    A portable barrier strip having retractable tire-puncture spikes for puncturing a vehicle tire. The tire-puncture spikes have an armed position for puncturing a tire and a retracted position for not puncturing a tire. The strip comprises a plurality of barrier blocks having the tire-puncture spikes removably disposed in a shaft that is rotatably disposed in each barrier block. The plurality of barrier blocks hare hingedly interconnected by complementary hinges integrally formed into the side of each barrier block which allow the strip to be rolled for easy storage and retrieval, but which prevent irregular or back bending of the strip. The shafts of adjacent barrier blocks are pivotally interconnected via a double hinged universal joint to accommodate irregularities in a roadway surface and to transmit torsional motion of the shaft from block to block. A single flexshaft cable is connected to the shaft of an end block to allow a user to selectively cause the shafts of a plurality of adjacently connected barrier blocks to rotate the tire-puncture spikes to the armed position for puncturing a vehicle tire, and to the retracted position for not puncturing the tire. The flexshaft is provided with a resiliently biased retracting mechanism, and a release latch for allowing the spikes to be quickly retracted after the intended vehicle tire is punctured.

  1. Blue light photoreceptors and methods of using the same

    DOE Patents [OSTI]

    Cashmore, Anthony Robert; Ahmad, Margaret; Lin, Chentao

    1998-01-01

    The invention features a substantially pure preparation of a nucleic acid encoding a HY4 or a HY4-related gene. The invention further features transgenic plants encoding a HY4 gene having a shorter stem than substantially homozygous wild type nontransgenic plants; and, transgenic plants comprising complementary HY4 sequences having a longer stem than substantially homozygous wild type nontransgenic plants.

  2. CYP2E1-dependent elevation of serum cholesterol, triglycerides, and hepatic bile acids by isoniazid

    SciTech Connect (OSTI)

    Cheng, Jie; Krausz, Kristopher W.; Li, Feng; Ma, Xiaochao; Gonzalez, Frank J.

    2013-01-15

    Isoniazid is the first-line medication in the prevention and treatment of tuberculosis. Isoniazid is known to have a biphasic effect on the inhibitioninduction of CYP2E1 and is also considered to be involved in isoniazid-induced hepatotoxicity. However, the full extent and mechanism of involvement of CYP2E1 in isoniazid-induced hepatotoxicity remain to be thoroughly investigated. In the current study, isoniazid was administered to wild-type and Cyp2e1-null mice to investigate the potential toxicity of isoniazid in vivo. The results revealed that isoniazid caused no hepatotoxicity in wild-type and Cyp2e1-null mice, but produced elevated serum cholesterol and triglycerides, and hepatic bile acids in wild-type mice, as well as decreased abundance of free fatty acids in wild-type mice and not in Cyp2e1-null mice. Metabolomic analysis demonstrated that production of isoniazid metabolites was elevated in wild-type mice along with a higher abundance of bile acids, bile acid metabolites, carnitine and carnitine derivatives; these were not observed in Cyp2e1-null mice. In addition, the enzymes responsible for bile acid synthesis were decreased and proteins involved in bile acid transport were significantly increased in wild-type mice. Lastly, treatment of targeted isoniazid metabolites to wild-type mice led to similar changes in cholesterol, triglycerides and free fatty acids. These findings suggest that while CYP2E1 is not involved in isoniazid-induced hepatotoxicity, while an isoniazid metabolite might play a role in isoniazid-induced cholestasis through enhancement of bile acid accumulation and mitochondria ?-oxidation. -- Highlights: ? Isoniazid metabolites were elevated only in wild-type mice. ? Isoniazid caused no hepatotoxicity in wild-type and Cyp2e1-null mice. ? Isoniazid elevated serum cholesterol and triglycerides, and hepatic bile acids. ? Bile acid transporters were significantly decreased in isoniazid-treated mice.

  3. Streaked x-ray spectrometer having a discrete selection of Bragg geometries for Omega

    SciTech Connect (OSTI)

    Millecchia, M.; Regan, S. P.; Bahr, R. E.; Romanofsky, M.; Sorce, C.

    2012-10-15

    The streaked x-ray spectrometer (SXS) is used with streak cameras [D. H. Kalantar, P. M. Bell, R. L. Costa, B. A. Hammel, O. L. Landen, T. J. Orzechowski, J. D. Hares, and A. K. L. Dymoke-Bradshaw, in 22nd International Congress on High-Speed Photography and Photonics, edited by D. L. Paisley and A. M. Frank (SPIE, Bellingham, WA, 1997), Vol. 2869, p. 680] positioned with a ten-inch manipulator on OMEGA [T. R. Boehly et al., Opt. Commun. 133, 495 (1997)] and OMEGA EP [L. J. Waxer et al., Presented at CLEO/QELS 2008, San Jose, CA, 4-9 May 2008 (Paper JThB1)] for time-resolved, x-ray spectroscopy of laser-produced plasmas in the 1.4- to 20-keV photon-energy range. These experiments require measuring a portion of this photon-energy range to monitor a particular emission or absorption feature of interest. The SXS relies on a pinned mechanical reference system to create a discrete set of Bragg reflection geometries for a variety of crystals. A wide selection of spectral windows is achieved accurately and efficiently using this technique. It replaces the previous spectrometer designs that had a continuous Bragg angle adjustment and required a tedious alignment calibration procedure. The number of spectral windows needed for the SXS was determined by studying the spectral ranges selected by OMEGA users over the last decade. These selections are easily configured in the SXS using one of the 25 discrete Bragg reflection geometries and one of the six types of Bragg crystals, including two curved crystals.

  4. Idaho Habitat/Natural Production Monitoring Part I, 1994 Annual Report.

    SciTech Connect (OSTI)

    Hall-Griswold, Judy A.; Leitzinger, Eric J.; Petrosky, C.E. (Idaho Department of Fish and Game, Boise, ID

    1995-11-01

    A total of 333 stream sections were sampled in 1994 to monitor in chinook salmon and steelhead trout parr populations in Idaho. Percent carry capacity and density estimates were summarized by different classes of fish: wild A-run steelhead trout, wild B-run steelhead trout, natural A-run steelhead trout, natural B-run steelhead trout, wild spring and summer chinook salmon. These data were also summarized by cells and subbasins as defined in Idaho Department of Fish and Game`s 1992-1996 Anadromous Fish Management Plan.

  5. Distribution of Clokey's Eggvetch

    SciTech Connect (OSTI)

    David C. Anderson

    1998-12-01

    The Environment, Safety and Health Division of the U.S. Department of Energy, Nevada Operations Office implements the Ecological Monitoring and Compliance Program on the Nevada Test Site (NTS). This program ensures compliance with applicable environmental laws and regulations, delineates and describes NTS ecosystems, and provides ecological information for predicting and evaluating potential impacts of proposed projects on those ecosystems. Over the last several decades, has taken an active role in providing information on the tatus of plant species proposed for protection under the Endangered Species Act(ESA). One such species is Clokey's eggvetch (Astragalus oophorus var. clokeyanus), which is a candidate species under the listing guidelines of the ESA. Surveys for this species were conducted on the NTS in 1996, 1997, and 1998. Field surveys focused on potential habitat for this species in the southern Belted range and expanded to other areas with similar habitat. Over 30 survey day s were completed; five survey days in 1996, 25 survey days in 1997, and three survey days in 1998. Clokey's eggvetch was located at several sites in the southern Belted Range. It was found through much of the northern section of Kawich Canyon, one site at the head of Gritty Gulch, and a rather extensive location in Lambs Canyon. It was also located further south at Captain Jack Springs in the Eleana Range, in much of Falcon Canyon and around Echo Peak on Pahute Mesa, and was also found in the Timber and Shoshone Mountains. Overall, the locations of Clokey's eggvetch on the NTS appears to form a distinct bridge between populations of the species located further north in the Belted and Kawich Ranges and the population located in the Spring Mountains. Clokey's eggvetch was commonly found along washes and small draws, and typically in sandy loam soils with a covering of light tuffaceous rock. It occurs primarily above 1830 meters (6000 feet) in association with single-leaf pinyon (Pinus

  6. Spotlights Archive | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    hosted a Northern Saw-Whet Owl banding demonstration by master bander, Tim Tolford. Bird banding is a technique used to study wild birds by attaching a tag to their leg to...

  7. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... K. ; Ishikawa, Tetsuya ; La Trobe) ; RIKEN) ; ESRF) June 2016 , Advances in X-Ray Optics;SPIE;129-139 Chemical analysis of Wild-2 samples returned by Stardust Flynn, G.J. ; ...

  8. 180C

    Office of Environmental Management (EM)

    Fragua Council of State Governments-ERC Conrad Smith P. Edwards R. Pinney M. Mulhare C. Richardson M. Vyenielo W. Sherman C. Smith C. Smith E. Wilds C. Smith W. Sherman J. ...

  9. SREL Reprint #3331

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    deer and wild boar within the Chernobyl exclusion zone are similar to those in four (uncontaminated) nature reserves in the region and wolf abundance is more than 7 times higher. ...

  10. Coal keeps the home fires burning, at a price

    SciTech Connect (OSTI)

    O'Connell, J.

    2007-11-15

    The wild ride of 2007 thermal and coking coal and freight prices does not show any signs of abating as 2008 nears, leaving consumers coping with historic high costs, except in the US. 3 figs.

  11. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... with a "wild" annual grass, Avena fatua, which is commonly a dominant plant in Mediterranean-type annual grasslands around the world; the plant was grown in its grassland soil. ...

  12. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    ... with a "wild" annual grass, Avena fatua, which is commonly a dominant plant in Mediterranean-type annual grasslands around the world; the plant was grown in its grassland soil. ...

  13. BPA-2014-01120-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Wild Salmon is a 501(c)(3) tax-exempt non-profit organization of more than 50 sport fishing, commercial fishing, and conservation organizations - local, regional, and...

  14. DOE/EIS-0005-FS-2

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Indian Affairs; Bureau of Land Management; Fish and Wildlife Service; and Geological ... The U . S . Fish and Wildli fe Service and several state agenc ies al so manage land in ...

  15. RAPID/Roadmap/12-WA-a | Open Energy Information

    Open Energy Info (EERE)

    take wildlife from the wild without permission from the Washington State Department of Fish and Wildlife (WDFW). The WDFW issues Live Wildlife Taking Permits under WAC 232-12-064....

  16. DOE - NNSA/NFO -- Plants and Animals

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plants & Animals NNSANFO Language Options U.S. DOENNSA - Nevada Field Office Click to subscribe to NNSS News Plants and Animals Wild Horses at the NNSS Because of its physical ...

  17. President Obama's Visit to Alaska: Closing Thoughts | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Guard cutters and the edges of ice fields, I've had the opportunity to see some wild and beautiful things in Alaska - and I've enjoyed sharing them with the rest of the country. ...

  18. Beasley Lab | Savannah River Ecology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AND DISEASE DYNAMICS OF WILD PIGS ON THE SAVANNAH RIVER SITE SREL Collaborators: Jim ... At the Savannah River Site alone between 30 and 40 pigs are struck by vehicles each year, ...

  19. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Lamarque, J F (3) Szopa, S (3) Wild, O (3) Atherton, C (2) Bergmann, D. (2) Cameron-Smith, P (2) Cameron-Smith, Philip (2) Cuvelier, C (2) Dalsoren, S. (2) Save Results Save this ...

  20. 2013-02 "Review Material Disposal Areas at LANL in Addition to...

    Broader source: Energy.gov (indexed) [DOE]

    2 is to ensure that the appropriate level of review has been given to MDAs other than MDA G at LANL that may be at similar risk in the event of wild fires. Rec 2013-02 - March 20,...

  1. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    We analyzed the response of soil bacteria to roots of the common annual grass Avena fatua ... associated with a "wild" annual grass, Avena fatua, which is commonly a dominant plant ...

  2. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... By the year 2500, there are complete recoveries of the globally averaged surface air ... Source-Receptor Relationships for Ozone Pollution Fiore, A M ; Dentener, F J ; Wild, O ; ...

  3. Form:Reference | Open Energy Information

    Open Energy Info (EERE)

    The reference title should match the book title. e.g.- Where the Wild Things Are Book Review Used when citing a review of a book. The reference title should include "Review of the...

  4. November 12, 2010

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Cafs November 12, 2010 November 12, 2010 Print Friday, 12 November 2010 00:00 christine beavers Having a ball in the wild world of fullerenes Christine Beavers, Beamline 11.3.1 ...

  5. DOI-BLM-NV-C010-2013-0037-DNA | Open Energy Information

    Open Energy Info (EERE)

    37-DNA Jump to: navigation, search NEPA Document Collection for: DOI-BLM-NV-C010-2013-0037-DNA DNA at Gabbs Valley Geothermal Area for GeothermalWell Field, DNA for Wild Rose Unit...

  6. User Data Forum Presentations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    M02: The Future of Data and Scientific Workflow June 16, 2014 | Author(s): Michael Wilde, ... MB M03: The Future of Large Scale Visual Data Analysis June 16, 2014 | Author(s): E. Wes ...

  7. User Data Forum Presentations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Presentations User Data Forum Presentations Sort by: Default | Name | Date (low-high) | ... M02: The Future of Data and Scientific Workflow June 16, 2014 | Author(s): Michael Wilde, ...

  8. BPA-2015-01485-FCorrespondence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FREEDOM OF INFORMATION ACT PROGRAM In reply refer to: FOIA BPA-2015-01485-F Arlene Montgomery Friends of the Wild Swan PO Box 103 Bigfork, MT 59911 Ms. Montgomery: The Department...

  9. Fermilab Today | Tip of the Week Archive

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sept. 28, 2015 Actions to avoid Sept. 21, 2015 Clean, green restrooms Sept. 14, 2015 Influenza 2015-16 Aug. 31, 2015 Our children depend on our safe driving Aug. 24, 2015 Wild...

  10. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three...

  11. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents,...

  12. Mar_13Times.indd

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Y-12 Employees' Soci- ety sport event, employees participated in outside-of-work activities: "Wild and Woolly Games Staged in Plant Loop," The Y-12 Bulletin, June 3, 1947, pg. 4. ...

  13. http://frwebgate.access.gpo.gov/cgi-bin/usc.cgi?ACTION=RETRIEVE

    National Nuclear Security Administration (NNSA)

    Congressional findings and declaration of policy Congress finds and declares that wild free-roaming horses and burros are living symbols of the historic and pioneer spirit of the...

  14. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    ... "VT","Chittenden",7601,"Green Mountain Power Corp",3737,"Essex Junction ... "WA","Kittitas",15500,"Puget Sound Energy Inc",56322,"Wild Horse",22,"WHS1",0.5,0.5...

  15. Cellobiohydrolase I enzymes

    DOE Patents [OSTI]

    Adney, William S; Himmel, Michael E; Decker, Stephen R; Knoshaug, Eric P; Nimlos, Mark R; Crowley, Michael F; Jeoh, Tina

    2014-01-28

    Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The mutations in the catalytic domain reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. The addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide increases O-linked glycosylation of the isolated polypeptide. Further provided are compositions comprising such polypeptides and nucleic acids encoding such polypeptides. Still further provided are methods for making such polypeptides.

  16. Mutant HNF-1{alpha} and mutant HNF-1{beta} identified in MODY3 and MODY5 downregulate DPP-IV gene expression in Caco-2 cells

    SciTech Connect (OSTI)

    Gu Ning [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Laboratory of Neurochemistry, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Adachi, Tetsuya [Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto (Japan); Matsunaga, Tetsuro [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Takeda, Jun [Department of Endocrinology Diabetes and Rheumatology, Graduate School of Medicine, Gifu University School of Medicine, Gifu (Japan); Tsujimoto, Gozoh [Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto (Japan); Ishihara, Akihiko [Laboratory of Neurochemistry, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Yasuda, Koichiro [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan); Diabetic Center, Tsunashimakai-Kosei Hospital, Himeji (Japan); Tsuda, Kinsuke [Laboratory of Metabolism, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto (Japan)]. E-mail: jinkan@tom.life.h.kyoto-u.ac.jp

    2006-08-04

    Dipeptidylpeptidase IV (DPP-IV) is a well-documented drug target for the treatment of type 2 diabetes. Hepatocyte nuclear factors (HNF)-1{alpha} and HNF-1{beta}, known as the causal genes of MODY3 and MODY5, respectively, have been reported to be involved in regulation of DPP-IV gene expression. But, it is not completely clear (i) that they play roles in regulation of DPP-IV gene expression, and (ii) whether DPP-IV gene activity is changed by mutant HNF-1{alpha} and mutant HNF-1{beta} in MODY3 and MODY5. To explore these questions, we investigated transactivation effects of wild HNF-1{alpha} and 13 mutant HNF-1{alpha}, as well as wild HNF-1{beta} and 2 mutant HNF-1{beta}, on DPP-IV promoter luciferase gene in Caco-2 cells by means of a transient experiment. Both wild HNF-1{alpha} and wild HNF-1{beta} significantly transactivated DPP-IV promoter, but mutant HNF-1{alpha} and mutant HNF-1{beta} exhibited low transactivation activity. Moreover, to study whether mutant HNF-1{alpha} and mutant HNF-1{beta} change endogenous DPP-IV enzyme activity, we produced four stable cell lines from Caco-2 cells, in which wild HNF-1{alpha} or wild HNF-1{beta}, or else respective dominant-negative mutant HNF-1{alpha}T539fsdelC or dominant-negative mutant HNF-1{beta}R177X, was stably expressed. We found that DPP-IV gene expression and enzyme activity were significantly increased in wild HNF-1{alpha} cells and wild HNF-1{beta} cells, whereas they decreased in HNF-1{alpha}T539fsdelC cells and HNF-1{beta}R177X cells, compared with DPP-IV gene expression and enzyme activity in Caco-2 cells. These results suggest that both wild HNF-1{alpha} and wild HNF-1{beta} have a stimulatory effect on DPP-IV gene expression, but that mutant HNF-1{alpha} and mutant HNF-1{beta} attenuate the stimulatory effect.

  17. Arsenic (+ 3 oxidation state) methyltransferase genotype affects steady-state distribution and clearance of arsenic in arsenate-treated mice

    SciTech Connect (OSTI)

    Hughes, Michael F.; Edwards, Brenda C.; Herbin-Davis, Karen M.; Saunders, Jesse; Styblo, Miroslav; Thomas, David J.

    2010-12-15

    Arsenic (+ 3 oxidation state) methyltransferase (As3mt) catalyzes formation of mono-, di-, and tri-methylated metabolites of inorganic arsenic. Distribution and retention of arsenic were compared in adult female As3mt knockout mice and wild-type C57BL/6 mice using a regimen in which mice received daily oral doses of 0.5 mg of arsenic as arsenate per kilogram of body weight. Regardless of genotype, arsenic body burdens attained steady state after 10 daily doses. At steady state, arsenic body burdens in As3mt knockout mice were 16 to 20 times greater than in wild-type mice. During the post dosing clearance period, arsenic body burdens declined in As3mt knockout mice to {approx} 35% and in wild-type mice to {approx} 10% of steady-state levels. Urinary concentration of arsenic was significantly lower in As3mt knockout mice than in wild-type mice. At steady state, As3mt knockout mice had significantly higher fractions of the body burden of arsenic in liver, kidney, and urinary bladder than did wild-type mice. These organs and lung had significantly higher arsenic concentrations than did corresponding organs from wild-type mice. Inorganic arsenic was the predominant species in tissues of As3mt knockout mice; tissues from wild-type mice contained mixtures of inorganic arsenic and its methylated metabolites. Diminished capacity for arsenic methylation in As3mt knockout mice prolongs retention of inorganic arsenic in tissues and affects whole body clearance of arsenic. Altered retention and tissue tropism of arsenic in As3mt knockout mice could affect the toxic or carcinogenic effects associated with exposure to this metalloid or its methylated metabolites.

  18. WILDFIRE IGNITION RESISTANCE ESTIMATOR WIZARD SOFTWARE DEVELOPMENT REPORT

    SciTech Connect (OSTI)

    Phillips, M.; Robinson, C.; Gupta, N.; Werth, D.

    2012-10-10

    This report describes the development of a software tool, entitled “WildFire Ignition Resistance Estimator Wizard” (WildFIRE Wizard, Version 2.10). This software was developed within the Wildfire Ignition Resistant Home Design (WIRHD) program, sponsored by the U. S. Department of Homeland Security, Science and Technology Directorate, Infrastructure Protection & Disaster Management Division. WildFIRE Wizard is a tool that enables homeowners to take preventive actions that will reduce their home’s vulnerability to wildfire ignition sources (i.e., embers, radiant heat, and direct flame impingement) well in advance of a wildfire event. This report describes the development of the software, its operation, its technical basis and calculations, and steps taken to verify its performance.

  19. Structure of adenovirus bound to cellular receptor car

    DOE Patents [OSTI]

    Freimuth, Paul I.

    2007-01-02

    Disclosed is a mutant CAR-DI-binding adenovirus which has a genome comprising one or more mutations in sequences which encode the fiber protein knob domain wherein the mutation causes the encoded viral particle to have a significantly weakened binding affinity for CAR-DI relative to wild-type adenovirus. Such mutations may be in sequences which encode either the AB loop, or the HI loop of the fiber protein knob domain. Specific residues and mutations are described. Also disclosed is a method for generating a mutant adenovirus which is characterized by a receptor binding affinity or specificity which differs substantially from wild type.

  20. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public

  1. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public

  2. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public

  3. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure and Receptor Specificity of an Avian Flu Antigen Structure and Receptor Specificity of an Avian Flu Antigen Print Wednesday, 25 July 2007 00:00 To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of

  4. CONSEQUENCES OF ALTERING RUBISCO REGULATION

    SciTech Connect (OSTI)

    Salvucci, Michael

    2013-12-31

    Research examined the thermal stability and propensity for aggregation of wild type and the C- and N-terminally modified forms of activase to determine if loss of activity under heat stress is dependent on protein aggregation. The results showed that 1) loss of activity at high temperature is independent of aggregation; 2) activase with both C- and N-terminal S-Tags are more susceptible to aggregation than wild type activase, 3) aggregation is highly dependent on the concentration of Mg2+ and 4) the ATP analog, ATPgammaS, protects against both thermal inactivation and aggregation.

  5. Divinyl ether synthase gene and protein, and uses thereof

    SciTech Connect (OSTI)

    Howe, Gregg A.; Itoh, Aya

    2011-09-13

    The present invention relates to divinyl ether synthase genes, proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also relates to methods of using divinyl ether synthase genes and proteins, including in their expression in transgenic organisms and in the production of divinyl ether fatty acids, and to methods of suing divinyl ether fatty acids, including in the protection of plants from pathogens.

  6. Divinyl ether synthase gene, and protein and uses thereof

    DOE Patents [OSTI]

    Howe, Gregg A.; Itoh, Aya

    2006-12-26

    The present invention relates to divinyl ether synthase genes, proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also relates to methods of using divinyl ether synthase genes and proteins, including in their expression in transgenic organisms and in the production of divinyl ether fatty acids, and to methods of suing divinyl ether fatty acids, including in the protection of plants from pathogens.

  7. Structure and Receptor Specificity of an Avian Flu Antigen

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Receptor Specificity of an Avian Flu Antigen Print To date, the H5N1 avian influenza viruses, which are currently circulating in domestic and wild birds on three continents, have only a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either by reassortment (the mixing of genetic material from similar viruses) or mutation, is seen as a major threat to public health

  8. Using weeds to fight wastes

    SciTech Connect (OSTI)

    1992-10-01

    Researchers at Los Alamos National Laboratory and New Mexico State University have discovered that jimson weed and wild tomato plants can remove the toxic wastes in wastewater associated with the production of trinitrotoluene (TNT). According to Wolfgang F. Mueller of New Mexico State, tissue-cultured cells of jimson weed rapidly absorb and break down toxic and carcinogenic elements in {open_quotes}pink water,{close_quotes} a by-product of the manufacture of TNT. Mueller and his colleagues have found similar results with the wild tomato plant.

  9. EA-1895: Lolo Creek Permanent Weir Construction near town of Weippe, Clearwater County, Idaho

    Broader source: Energy.gov [DOE]

    DOE’s Bonneville Power Administration was preparing this EA to evaluate the potential environmental impacts of replacing an existing seasonal fish weir with a permanent weir, which would have been used to monitor federally-listed Snake River steelhead and collect spring Chinook salmon adults to support ongoing supplementation programs in the watershed. The Bureau of Land Management, a cooperating agency, preliminarily determined Lolo Creek to be suitable for Congressional designation into the Wild and Scenic River System. The EA included a Wild and Scenic River Section 7 analysis. This project was canceled.

  10. Keep in mind, that with formality, often comes more of a standing presence of a committee within DOE-it gets into DOE's system and becomes a budget line item as well

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ICEIWG May 29, 2013 WILD HORSE PASS HOTEL & CASINO 5040 Wild Horse Pass Blvd., Chandler, AZ AGENDA CONFERENCE ROOM: ACACIA CD WEDNESDAY, MAY 29, 2013 8:00-9:00am REGISTRATION & BREAKFAST ** Continental breakfast will be provided on-site. ** 9:00-11:00am WELCOME & INTRODUCTIONS Opening Prayer by ICEIWG Tribal Representative Tracey A. LeBeau, Director, U.S. DOE Office of Indian Energy (IE) & DOE ICEIWG Co-Chair Jim Manion, Warm Springs Delegate & Tribal ICEIWG Co-Chair Pilar