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  1. Refugio County, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Refugio County, Texas: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 28.3465339, -97.1359377 Show Map Loading map... "minzoom":false,"mapping...

  2. Nebraska Nuclear Profile - Fort Calhoun

    U.S. Energy Information Administration (EIA) Indexed Site

    Fort Calhoun" "Unit","Summer Capacity (MW)","Net Generation (Thousand MWh)","Summer Capacity Factor (Percent)","Type","Commercial Operation Date","License Expiration Date" 1,478,"3,701",88.4,"PWR","application/vnd.ms-excel","application/vnd.ms-excel" ,478,"3,701",88.4

  3. Calhoun County, South Carolina: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Climate Zone Number 3 Climate Zone Subtype A. Places in Calhoun County, South Carolina Cameron, South Carolina St. Matthews, South Carolina Retrieved from "http:en.openei.orgw...

  4. Calhoun County Elec Coop Assn | Open Energy Information

    Open Energy Info (EERE)

    Facebook: https:www.facebook.compagesCalhoun-County-REC173498466069004?skwall Outage Hotline: 800-821-4879 Outage Map: www.iowarec.orgoutages References: EIA Form...

  5. Calhoun County, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Number 2 Climate Zone Subtype A. Places in Calhoun County, Texas Point Comfort, Texas Port Lavaca, Texas Seadrift, Texas Retrieved from "http:en.openei.orgw...

  6. Victoria Transport Policy Institute | Open Energy Information

    Open Energy Info (EERE)

    Transport Policy Institute Jump to: navigation, search Name: Victoria Transport Policy Institute Address: 1250 Rudlin Street, Place: Victoria, British Columbia Website:...

  7. Victoria, Australia: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Victoria, Australia: Energy Resources Jump to: navigation, search Name Victoria, Australia Equivalent URI DBpedia GeoNames ID 2145234 Coordinates -37, 145 Show Map Loading...

  8. Selection of area and specific site for drilling a horizontal well in Calhoun County, West Virginia

    SciTech Connect (OSTI)

    Reeves, T.K.; Overbey, W.K. Jr.; Salamy, S.P.; Locke, C.D.

    1992-03-01

    This report discusses the data collection and analysis procedures used to establish criteria for geologic and engineering studies conducted by BDM to select a general area for more detailed study and a specific site for the drilling of a cooperative well with an industry partner, the Consolidated Natural Gas Development Company (CNGD). The results of detailed geologic studies are presented for two areas in Calhoun County, West Virginia, and one area along the Logan-Boone County line in West Virginia. The effects of Appalachian Basin tectonics and the Rome Trough Rift system were identified on seismic lines made available by (CNGD). These helped to identify and define the trapping mechanisms which had been effective in each area. Engineering analyses of past production histories provided data to support selection of target areas and then to select a specific site that met the project requirements for production, reservoir pressure, and risk. A final site was selected in Lee District at the southwestern margin of the Sand Ridge gas field based on the combination of a geologic trapping mechanism and reservoir pressures which were projected as 580 psi with a stress ratio of 0.53.

  9. Recent plant studies using Victoria 2.0

    SciTech Connect (OSTI)

    BIXLER,NATHAN E.; GASSER,RONALD D.

    2000-03-08

    VICTORIA 2.0 is a mechanistic computer code designed to analyze fission product behavior within the reactor coolant system (RCS) during a severe nuclear reactor accident. It provides detailed predictions of the release of radioactive and nonradioactive materials from the reactor core and transport and deposition of these materials within the RCS and secondary circuits. These predictions account for the chemical and aerosol processes that affect radionuclide behavior. VICTORIA 2.0 was released in early 1999; a new version VICTORIA 2.1, is now under development. The largest improvements in VICTORIA 2.1 are connected with the thermochemical database, which is being revised and expanded following the recommendations of a peer review. Three risk-significant severe accident sequences have recently been investigated using the VICTORIA 2.0 code. The focus here is on how various chemistry options affect the predictions. Additionally, the VICTORIA predictions are compared with ones made using the MELCOR code. The three sequences are a station blackout in a GE BWR and steam generator tube rupture (SGTR) and pump-seal LOCA sequences in a 3-loop Westinghouse PWR. These sequences cover a range of system pressures, from fully depressurized to full system pressure. The chief results of this study are the fission product fractions that are retained in the core, RCS, secondary, and containment and the fractions that are released into the environment.

  10. Drilling, completion, stimulation, and testing of BDM/CNGD Well 3997, Lee District, Calhoun County, West Virginia

    SciTech Connect (OSTI)

    Overbey, W.K. Jr.; Carden, R.S.; Salamy, S.P.; Locke, C.D.; Johnson, H.R.

    1992-03-01

    This report discusses the detailed field operations in drilling, casing, completing, and stimulating the Hunter Bennett No. 3997 well located in Lee District, Calhoun County West Virginia. The project was designed and managed by BDM in cooperation with CNG Development Company. The well was spudded on November 9, 1990, and drilling was completed on December 14, 1990. The well was drilled on an average asmuth of 312 degrees with a total horizontal displacement of 2459 feet. The well was turned to a 90 degree inclination from the vertical over a measured course length of 1216 feet. Approximately 1381 feet of the well had an inclination higher than 86 degrees, while 2179 feet had an inclination greater than 62 degrees. The well was partitioned into five zones for stimulation purposes. Each zone is a little more than 300 feet long. The well was stimulated with nitrogen gas in zones one and two. Early production results are encouraging. The BDM/CNGD horizontal well averaged 147 mcfd of gas over the first week of production and, in week five, began to produce oil at a rate of about 2 bbl/day.

  11. Drilling, completion, stimulation, and testing of BDM/CNGD Well 3997, Lee District, Calhoun County, West Virginia. Final report

    SciTech Connect (OSTI)

    Overbey, W.K. Jr.; Carden, R.S.; Salamy, S.P.; Locke, C.D.; Johnson, H.R.

    1992-03-01

    This report discusses the detailed field operations in drilling, casing, completing, and stimulating the Hunter Bennett No. 3997 well located in Lee District, Calhoun County West Virginia. The project was designed and managed by BDM in cooperation with CNG Development Company. The well was spudded on November 9, 1990, and drilling was completed on December 14, 1990. The well was drilled on an average asmuth of 312 degrees with a total horizontal displacement of 2459 feet. The well was turned to a 90 degree inclination from the vertical over a measured course length of 1216 feet. Approximately 1381 feet of the well had an inclination higher than 86 degrees, while 2179 feet had an inclination greater than 62 degrees. The well was partitioned into five zones for stimulation purposes. Each zone is a little more than 300 feet long. The well was stimulated with nitrogen gas in zones one and two. Early production results are encouraging. The BDM/CNGD horizontal well averaged 147 mcfd of gas over the first week of production and, in week five, began to produce oil at a rate of about 2 bbl/day.

  12. VICTORIA: A mechanistic model of radionuclide behavior in the reactor coolant system under severe accident conditions

    SciTech Connect (OSTI)

    Heames, T.J. ); Williams, D.A.; Johns, N.A.; Chown, N.M. ); Bixler, N.E.; Grimley, A.J. ); Wheatley, C.J. )

    1990-10-01

    This document provides a description of a model of the radionuclide behavior in the reactor coolant system (RCS) of a light water reactor during a severe accident. This document serves as the user's manual for the computer code called VICTORIA, based upon the model. The VICTORIA code predicts fission product release from the fuel, chemical reactions between fission products and structural materials, vapor and aerosol behavior, and fission product decay heating. This document provides a detailed description of each part of the implementation of the model into VICTORIA, the numerical algorithms used, and the correlations and thermochemical data necessary for determining a solution. A description of the code structure, input and output, and a sample problem are provided. The VICTORIA code was developed upon a CRAY-XMP at Sandia National Laboratories in the USA and a CRAY-2 and various SUN workstations at the Winfrith Technology Centre in England. 60 refs.

  13. Victorias energy efficiency and cogeneration project. Final report

    SciTech Connect (OSTI)

    1998-10-31

    This report describes a two-phase energy project currently contemplated for joint implementation at the Victorias Milling Company, a large sugar mill and refinery on the island of Negros in the Visayas region of the Philippines. The Energy Efficiency (EE) phase is expected to reduce of eliminate VMC`s fossil fuel consumption, which will have a direct and substantial impact on carbon emissions. Phase I is an EE project which involves the installation of equipment to reduce steam and electricity demand in the factories. Phase II, will involve retrofitting and increasing the capacity of the steam and power generation systems, and selling power to the grid. By increasing efficiency and output, the cogeneration project will allow the factory to use only bagasse sugar cane fiber waste as fuel for energy needs. The cogeneration project will also eliminate VMC`s electricity purchases and supply additional power for the island, which will offset generation capacity expansion on the island and the Visayas region.

  14. Nuclear forensic analysis of uranium oxide powders interdicted in Victoria, Australia

    SciTech Connect (OSTI)

    Kristo, Michael Joseph; Keegan, Elizabeth; Colella, Michael; Williams, Ross; Lindvall, Rachel; Eppich, Gary; Roberts, Sarah; Borg, Lars; Gaffney, Amy; Plaue, Jonathan; Knight, Kim; Loi, Elaine; Hotchkis, Michael; Moody, Kenton; Singleton, Michael; Robel, Martin; Hutcheon, Ian

    2015-04-13

    Abstract

    Nuclear forensic analysis was conducted on two uranium samples confiscated during a police investigation in Victoria, Australia. The first sample, designated NSR-F-270409-1, was a depleted uranium powder of moderate purity (∼ 1000 μg/g total elemental impurities). The chemical form of the uranium was a compound similar to K

  15. VICTORIA: A mechanistic model of radionuclide behavior in the reactor coolant system under severe accident conditions. Revision 1

    SciTech Connect (OSTI)

    Heams, T J; Williams, D A; Johns, N A; Mason, A; Bixler, N E; Grimley, A J; Wheatley, C J; Dickson, L W; Osborn-Lee, I; Domagala, P; Zawadzki, S; Rest, J; Alexander, C A; Lee, R Y

    1992-12-01

    The VICTORIA model of radionuclide behavior in the reactor coolant system (RCS) of a light water reactor during a severe accident is described. It has been developed by the USNRC to define the radionuclide phenomena and processes that must be considered in systems-level models used for integrated analyses of severe accident source terms. The VICTORIA code, based upon this model, predicts fission product release from the fuel, chemical reactions involving fission products, vapor and aerosol behavior, and fission product decay heating. Also included is a detailed description of how the model is implemented in VICTORIA, the numerical algorithms used, and the correlations and thermochemical data necessary for determining a solution. A description of the code structure, input and output, and a sample problem are provided.

  16. Nuclear forensic analysis of uranium oxide powders interdicted in Victoria, Australia

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kristo, Michael Joseph; Keegan, Elizabeth; Colella, Michael; Williams, Ross; Lindvall, Rachel; Eppich, Gary; Roberts, Sarah; Borg, Lars; Gaffney, Amy; Plaue, Jonathan; et al

    2015-04-13

    Nuclear forensic analysis was conducted on two uranium samples confiscated during a police investigation in Victoria, Australia. The first sample, designated NSR-F-270409-1, was a depleted uranium powder of moderate purity (~1000 μg/g total elemental impurities). The chemical form of the uranium was a compound similar to K2(UO2)3O4·4H2O. While aliquoting NSR-F-270409-1 for analysis, the body and head of a Tineid moth was discovered in the sample. The second sample, designated NSR-F-270409-2, was also a depleted uranium powder. It was of reasonably high purity (~380 μg/g total elemental impurities). The chemical form of the uranium was primarily UO3·2H2O, with minor phases ofmore » U3O8 and UO2. While aliquoting NSR-F-270409-2 for analysis, a metal staple of unknown origin was discovered in the sample. The presence of 236U and 232U in both samples indicates that the uranium feed stocks for these samples experienced a neutron flux at some point in their history. The reactor burn-up calculated from the isotopic composition of the uranium is consistent with that of spent fuel from natural uranium (NU) fueled Pu production. These nuclear forensic conclusions allow us to categorically exclude Australia as the origin of the material and greatly reduce the number of candidate sources.« less

  17. Nuclear forensic analysis of uranium oxide powders interdicted in Victoria, Australia

    SciTech Connect (OSTI)

    Kristo, Michael Joseph; Keegan, Elizabeth; Colella, Michael; Williams, Ross; Lindvall, Rachel; Eppich, Gary; Roberts, Sarah; Borg, Lars; Gaffney, Amy; Plaue, Jonathan; Knight, Kim; Loi, Elaine; Hotchkis, Michael; Moody, Kenton; Singleton, Michael; Robel, Martin; Hutcheon, Ian

    2015-04-13

    Nuclear forensic analysis was conducted on two uranium samples confiscated during a police investigation in Victoria, Australia. The first sample, designated NSR-F-270409-1, was a depleted uranium powder of moderate purity (~1000 μg/g total elemental impurities). The chemical form of the uranium was a compound similar to K2(UO2)3O4·4H2O. While aliquoting NSR-F-270409-1 for analysis, the body and head of a Tineid moth was discovered in the sample. The second sample, designated NSR-F-270409-2, was also a depleted uranium powder. It was of reasonably high purity (~380 μg/g total elemental impurities). The chemical form of the uranium was primarily UO3·2H2O, with minor phases of U3O8 and UO2. While aliquoting NSR-F-270409-2 for analysis, a metal staple of unknown origin was discovered in the sample. The presence of 236U and 232U in both samples indicates that the uranium feed stocks for these samples experienced a neutron flux at some point in their history. The reactor burn-up calculated from the isotopic composition of the uranium is consistent with that of spent fuel from natural uranium (NU) fueled Pu production. These nuclear forensic conclusions allow us to categorically exclude Australia as the origin of the material and greatly reduce the number of candidate sources.

  18. Fluvial sedimentology and basin analyses of the Permian Fairchild and Buckley formations, Beardmore Glacier region, and the Weller Coal Measures, southern Victoria Land, Antarctica

    SciTech Connect (OSTI)

    Isbell, J.L.

    1990-01-01

    The Beardmore Glacier region contains a 1-km-thick Permian fluvial sequence that was deposited in an elongate basin along the margin of the East Antarctica craton. Fluvial architecture, sandstone composition and paleocurrents within the basin record a change from an early Permian cratonic to a late Permian foreland basin. The Lower Permian Fairchild Formation consists entirely of overlapping channel-form sandstone bodies deposited by braided streams. Arkosic sandstone was deposited by SE flowing streams. Fairchild strata record slow subsidence within a broad cratonic basin. The Lower to Upper Permian Buckley Formation consists of an arkosic lower member and a volcaniclastic upper member. Paleocurrents which consist of transverse and longitudinal paleocurrents, suggest a cratonward migration of the basin axis through time. The Buckley Formation was deposited within a braided stream setting and is an important unit because it contains interstratified channel-sandstone sheets, shale and coal, along with evidence of channel-belt avulsions. Sandstone sheets predominate at the base of the formation, while flood-plain deposits thicken and increase in abundance upward. The interaction between fluvial processes and subsidence rates produced this alluvial stratigraphy. The Lower Permian Weller Coal Measures in southern Victoria Land were deposited within a narrow basin located cratonward of the foreland basin. Basin geometry and depositional patterns are similar to those of fault-bounded basins. Although basin formation is not constrained, deposition of the Weller was contemporaneous with the development of the foreland basin. This suggests a relationship between subsidence within the two basins.

  19. Calhoun County, Iowa: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Jolley, Iowa Knierim, Iowa Lake City, Iowa Lohrville, Iowa Lytton, Iowa Manson, Iowa Pomeroy, Iowa Rinard, Iowa Rockwell City, Iowa Somers, Iowa Yetter, Iowa Retrieved from...

  20. Calhoun County, Alabama: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Alabama Glencoe, Alabama Hobson City, Alabama Jacksonville, Alabama Ohatchee, Alabama Oxford, Alabama Piedmont, Alabama Saks, Alabama Southside, Alabama Weaver, Alabama West...

  1. Calhoun County, Illinois: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    lse,"poi":true,"imageoverlays":,"markercluster":false,"searchmarkers":"","locations":"text":"","title":"","link":null,"lat":39.1733216,"lon":-90.658333,"alt":0,"address":"","ic...

  2. Calhoun County, Florida: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    lse,"poi":true,"imageoverlays":,"markercluster":false,"searchmarkers":"","locations":"text":"","title":"","link":null,"lat":30.3475302,"lon":-85.1894045,"alt":0,"address":"","i...

  3. Calhoun County, West Virginia: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    lse,"poi":true,"imageoverlays":,"markercluster":false,"searchmarkers":"","locations":"text":"","title":"","link":null,"lat":38.857973,"lon":-81.1196075,"alt":0,"address":"","ic...

  4. EIS-0494: Excelerate Liquefaction Solutions Lavaca Bay LNG Project, Calhoun and Jackson Counties, Texas

    Broader source: Energy.gov [DOE]

    The Federal Energy Regulatory Commission (FERC) is preparing, with DOE as a cooperating agency, an EIS to analyze the potential environmental impacts of a proposal to construct and operate a liquefied natural gas terminal consisting of two floating liquefaction, storage and offloading units and a 29-mile pipeline header system to transport natural gas from existing pipeline systems to the LNG terminal facilities.

  5. Uganda-Demonstrating Wind and Solar Energy on Lake Victoria ...

    Open Energy Info (EERE)

    programs, Background analysis, Technology characterizations Resource Type Guidemanual, Lessons learnedbest practices Website http:sgp.undp.orgdownloadS Country Uganda UN...

  6. Resumption of exploratory drilling operations by Texaco, Inc. : state oil and gas lesses - PRC 2725. 1 (Jade Prospect), PRC 2206. 1 (Anita Prospect), PRC 2955. 1 (Refugio Prospect), Santa Barbara County. Final environmental impact report

    SciTech Connect (OSTI)

    Not Available

    1982-04-01

    An update is presented on air quality conditions in Santa Barbara County, Califorina. Data on photochemical oxidants (measured as ozone), nitrogen dioxide, sulfide dioxide, carbon monoxide, and total suspended particulates are included. Also included are the comments received during the public review and during the public hearing on the Draft Environmental Impact Report on Texaco, Inc.'s proposed Resumption of Exploratory Drilling Operations, State Oil and Gas Leases PRC 2725.1, PRC 2206.1 and PRC 2955.1, responses to these written and oral (hearing) comments, and a modification to the Draft EIR Air Quality Setting section, incorporating updated ambient air quality data. This volume, when added to the Draft Environmental Impact Report, constitutes the Final Environmental Impact Report in accordance with the California Environmental Quality Act (CEQA) and the State Lands Commission's regulations implementing CEQA.

  7. Washington County, Nebraska: Energy Resources | Open Energy Informatio...

    Open Energy Info (EERE)

    Washington County, Nebraska Arlington, Nebraska Blair, Nebraska Fort Calhoun, Nebraska Herman, Nebraska Kennard, Nebraska Washington, Nebraska Retrieved from "http:en.openei.org...

  8. Alabama Residential Energy Code Field Study

    Broader source: Energy.gov [DOE]

    Lead Performer: Institute for Market Transformation – Washington, DCPartners: Alabama Center for Excellence in Clean Energy Technology, Calhoun Community College – Decatur, ALDOE Total Funding: ...

  9. EIS-0494: Notice of Intent to Prepare an Environmental Impact...

    Office of Environmental Management (EM)

    Excelerate Liquefaction Solutions Lavaca Bay LNG Project, Calhoun and Jackson Counties, Texas The Federal Energy Regulatory Commission (FERC) is preparing, with DOE as a ...

  10. Future Energy Pty Ltd | Open Energy Information

    Open Energy Info (EERE)

    Pty Ltd Jump to: navigation, search Name: Future Energy Pty Ltd Place: Victoria, Australia Zip: 3121 Sector: Wind energy Product: Victoria based community wind project developer....

  11. Centre for Energy and Greenhouse Technologies | Open Energy Informatio...

    Open Energy Info (EERE)

    200px Name: Centre for Energy and Greenhouse Technologies Address: 50 Northways Rd, Churchill Victoria 3842, Australia Place: Churchill Victoria, Australia Website:...

  12. CX-008412: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Alabama-County-Calhoun CX(s) Applied: A9, A11, B2.5, B5.1 Date: 07/03/2012 Location(s): Alabama Offices(s): Energy Efficiency and Renewable Energy

  13. North Georgia Elec Member Corp | Open Energy Information

    Open Energy Info (EERE)

    navigation, search Name: North Georgia Elec Member Corp Place: Georgia Phone Number: Dalton: (706) 259-9441; Fort Oglethorpe: (706) 866-2231; Calhoun: (706) 629-3160; Trion:...

  14. CX-005731: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Seadrift Wind TurbineCX(s) Applied: B5.1Date: 04/21/2011Location(s): Calhoun County, TexasOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  15. Atmos. Meas. Tech., 9, 1653-1669, ...

    Office of Scientific and Technical Information (OSTI)

    ... Soc., 94, 883-902, doi:10.1175BAMS-b-11-00057.1,2013. Barthelmie, R. J., Crippa, P., Wang, H., Smith, C. M., Krish- namurthy, R., Choukulkar, A., Calhoun, R., Valyou, b., Mar- ...

  16. DOE Webinar: Public Relations for Energy Sustainability-A Framework for Engaging Your Stakeholders Productively Around New Energy Projects

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE Webinar Public Relations for Energy Sustainability A framework for engaging your stakeholders productively around new energy projects Lisa Calhoun CEO, Write2Market   Leads a team of experts in getting energy and tech companies the reputations they deserve  Invented Triple A Industry Leadership methodology  University of Texas (MBA), Baylor (BA) @lisa_calhoun or @write2market Why are sustainability projects different? Be the forum for debate Reach out to both sides and

  17. CX-007044: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Texas-City-VictoriaCX(s) Applied: A1, B2.5, B5.1Date: 04/05/2010Location(s): Victoria, TexasOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  18. Carver County, Minnesota: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Cologne, Minnesota Hamburg, Minnesota Mayer, Minnesota New Germany, Minnesota Norwood Young America, Minnesota Victoria, Minnesota Waconia, Minnesota Watertown, Minnesota...

  19. CX-003773: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    State Energy Program - Clean Energy Property RebateCX(s) Applied: B4.11, B5.1Date: 09/07/2010Location(s): Calhoun, GeorgiaOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  20. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Darwin ARCS3 P. T. May, T. D. Keenan, and C. J. Jakob Bureau of Meteorological Research Center Melbourne 3001, Victoria, Australia B. Forgan Australian Bureau of Meteorology Melbourne 3001, Victoria, Australia R. Mitchell, S. A. Young, and M. Platt Commonwealth Scientific and Industrial Research Organization Aspendale, Victoria, Australia Introduction Darwin is located near 12°S, 131°E and the northern coast of Australia and is affected by a classic mon- soonal environment within the

  1. SREL Reprint #3073

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in Ugandan P. m. victoriae. Keywords: African cichlid, enrichment, population genetics, primer design, sequencing, tetranucleotide SREL Reprint 3073 Crispo, E., C. Hagen,...

  2. International Air Transport Association (IATA) | Open Energy...

    Open Energy Info (EERE)

    Name: International Air Transport Association (IATA) Address: 800 Place Victoria PO Box 113 Place: Montreal, Quebec Phone Number: 1 514 874 0202 Website: www.iata.org...

  3. Developing Adnectins That Target SRC Co-Activator Binding to...

    Office of Scientific and Technical Information (OSTI)

    ; Tebben, Andrew J. ; Wensel, David L. ; Wright, Martin C. ; Su, Julie ; Jenny, Victoria ; Gupta, Ruchira Das ; Ruzanov, Max ; Russo, Katie A. ; Bell, Aneka ; An, Yongmi ; Bryson, ...

  4. PREDICTION OF 4nu=1 RESONANCE OF A HIGH INTENSITY LINAC (Conference...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Conference Resource Relation: Conference: LINAC 08 conference, Victoria, Canada, 20080929, 20081003 Research Org: Oak Ridge National Laboratory (ORNL); Spallation ...

  5. TRANSVERSE MATCHING OF THE SNS LINAC BASED ON PROFILE MEASUREMENTS...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Conference Resource Relation: Conference: LINAC 08 conference, Victoria, Canada, 20080929, 20081003 Research Org: Oak Ridge National Laboratory (ORNL); Spallation ...

  6. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Victoria (3) Iversen, Colleen M (2) Norby, Richard (2) Sullivan, Patrick F. (2) Allen, Michael F. (1) Brooks, Jonathan (1) Childs, Joanne (1) Curtis, Bryan (1) Eissenstat, David...

  7. Patterns of Nitrogen Utilization in Deep-Sea Syntrophic Consortia (2010 JGI User Meeting)

    ScienceCinema (OSTI)

    Wiegel, Detlef

    2011-04-26

    Victoria Orphan from Caltech discusses "Patterns of nitrogen utilization in deep-sea syntrophic consortia" on March 25, 2010 at the 5th Annual DOE JGI User Meeting

  8. Transportation Demand Management (TDM) Encyclopedia | Open Energy...

    Open Energy Info (EERE)

    Implementation Resource Type: Guidemanual Website: www.vtpi.orgtdmtdm12.htm Cost: Free Language: English References: Victoria Transport Policy Institute1 "The Online TDM...

  9. seliger | The Ames Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    seliger Ames Laboratory Profile Victoria Seliger Custodian I Facilities Services 241C Metals Development Phone Number: 515-294-4360 Email Address: seliger

  10. Feb. 7 Science Series Lecturer to Discuss Living & Working in...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - the extreme cold of the Arctic. Guest speaker Victoria Hill, an oceanographer with Old Dominion University's bio-optics group, will talk about the work she and colleagues...

  11. EA-1602: Finding of No Significant Impact | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Under the Proposed Action, Western a would provide electric service to the AIP power substation for operating AIP project facilities, including the new pump station on Victoria ...

  12. Diamond Energy Pty Ltd | Open Energy Information

    Open Energy Info (EERE)

    Pty Ltd Jump to: navigation, search Name: Diamond Energy Pty Ltd Place: Melbourne, Australia Zip: 3124 Product: Victoria based clean energy project developer. Coordinates:...

  13. NUE Pty Ltd aka NU Energy | Open Energy Information

    Open Energy Info (EERE)

    Energy Jump to: navigation, search Name: NUE Pty Ltd (aka NU Energy) Place: Victoria, Australia Product: Australia-based energy systems integrator with the capabaility to design...

  14. Offshore Wind Market and Economic Analysis Report 2013

    Office of Environmental Management (EM)

    Jeff Anthony and Chris Long Great Lakes Wind Collaborative Victoria Pebbles Green Giraffe Energy Bankers Marie de Graaf, Jrme Guillet, and Niels Jongste National Renewable ...

  15. Plant Root Characteristics and Dynamics in Arctic Tundra Ecosystems...

    Office of Scientific and Technical Information (OSTI)

    and dynamics, and their role in key ecosystem processes in the Arctic. Authors: Sullivan, Paddy ; Sloan, Victoria ; Warren, Jeff ; McGuire, Dave ; Euskirchen, Eugenie ;...

  16. Molecular Foundry

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Synthesis LMKlivansky@lbl.gov 510.486.4199 Biography Education B.Sc.(HONS) Chemistry, Monash University, Victoria, Australia, 1990 Previous Professional Positions...

  17. Secretary Chu Meets with South Carolina Small Businesses | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy with South Carolina Small Businesses Secretary Chu Meets with South Carolina Small Businesses August 24, 2011 - 3:15pm Addthis Congressman James Clyburn speaks to South Carolina small business owners | Courtesy of Damien LaVera Congressman James Clyburn speaks to South Carolina small business owners | Courtesy of Damien LaVera Niketa Kumar Niketa Kumar Public Affairs Specialist, Office of Public Affairs South Carolina's Orangeburg-Calhoun Technical College is helping to leverage local

  18. FY 2012 Budget Request Advanced Research Projects Agency - Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nuclear Energy Improving the Economics of America's Nuclear Power Plants June 17, 2016 NEAC Meeting Bradley Williams Senior Advisor, Office of Nuclear Energy U.S. Department of Energy Challenges and Changes to U.S. Nuclear Fleet * Fitzpatrick, Pilgrim, Oyster Creek, Fort Calhoun slated to close * Clinton, Quad Cities, Nine Mile Point-1, Ginna recently announced plans to close * Several others at "at-risk" especially in deregulated markets * NEI estimates another 15-20 plants at risk

  19. Nebraska Nuclear Profile - Power Plants

    U.S. Energy Information Administration (EIA) Indexed Site

    Nebraska nuclear power plants, summer capacity and net generation, 2010" "Plant name/total reactors","Summer capacity (mw)","Net generation (thousand mwh)","Share of State nuclear net generation (percent)","Owner" "Cooper Unit 1",767,"6,793",61.4,"Nebraska Public Power District" "Fort Calhoun Unit 1",478,"4,261",38.6,"Omaha Public Power District" "2 Plants 2

  20. TEC/WG Tribal Topic Group

    Office of Environmental Management (EM)

    November 15, 2004 Participants: Nancy Bennett (UNM/ATRI), Vicki Best (YMPO), Kevin Blackwell (FRA), Tony Bouve (FRA), Mike Calhoun (FRA), Greg Fasano (SAIC), Ed Gonzales (ELG), Elizabeth Helvey (BSC), Dennis Hurtt (DOE/CBFO), Jay Jones (DOE/RW), Dan King (Oneida Nation), Bob Lupton (DOE/OCRWM), Julie Offner (BAH), Ellen Ott (DOE/GC), Jennifer Patric (BAH),Wilda Portner (SAIC), Lisa Sattler (CSG/MW), Linda Sikkema (NCSL), Steve White (NAEMT) Jay Jones (DOE/RW) chaired the call, which focused on

  1. Sandia National Laboratories and Internships

    Broader source: Energy.gov (indexed) [DOE]

    Sandra Begay-Campbell SNL Roger Taylor NREL Lizana Pierce DOE, Project Manager Victoria DeHerrera Navarro, Sr. Project Analyst Scott Haase NREL Sandra Begay-Campbell *Navajo ...

  2. BPA-2010-02131-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Atterbury,Laura M - DK-7 From: victoria leuba@ecy.wa.gov Sent: Friday, September 24, 2010 10:53 AM To: FOIA Subject: FOIA Request RECEWFU KN hi' 1 FOIA OFF't('E THIS DUE DATE: The...

  3. Soltek Powersource Ltd SPS Energy | Open Energy Information

    Open Energy Info (EERE)

    search Name: Soltek Powersource Ltd (SPS Energy) Place: Victoria, British Columbia, Canada Zip: V8M 2A4 Sector: Solar Product: Manufacturer and supplier of solar power systems...

  4. MHK Projects/bioWAVE Pilot Plant | Open Energy Information

    Open Energy Info (EERE)

    Project Phase Phase 1 Project Details A 250kW pilot project is being developed at Port Fairy Victoria, Australia,which will be connected to the national power distribution...

  5. BPA-2013-01582-FOIA Response

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Srinivasan Kailasam Zinfra Group 610 Heatherton Road Clayton South, Victoria 3169 Australia FOIA BPA-2013-01582-F Dear Mr. Kailasam: Thank you for your request for records that...

  6. The unseen iceberg: Plant roots in arctic tundra (Journal Article...

    Office of Scientific and Technical Information (OSTI)

    ecosystems in the Arctic. Authors: Iversen, Colleen M 1 ; Sloan, Victoria L 1 ; Sullivan, Patrick F. 2 ; Euskirchen, Eugenie S 2 ; McGuire, A. David 2 ; Norby, Richard...

  7. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    (1) Iversen, Colleen (1) McGuire, Dave (1) Norby, Richard (1) Sloan, Victoria (1) Sullivan, Paddy (1) Walker, Anthony (1) Warren, Jeff (1) Wullschleger, Stan (1) Save Results...

  8. Physiochemical Evidence of Faulting Processes and Modeling of Fluid in Evolving Fault Systems in Southern California

    SciTech Connect (OSTI)

    Boles, James

    2013-05-24

    Our study targets recent (Plio-Pleistocene) faults and young (Tertiary) petroleum fields in southern California. Faults include the Refugio Fault in the Transverse Ranges, the Ellwood Fault in the Santa Barbara Channel, and most recently the Newport- Inglewood in the Los Angeles Basin. Subsurface core and tubing scale samples, outcrop samples, well logs, reservoir properties, pore pressures, fluid compositions, and published structural-seismic sections have been used to characterize the tectonic/diagenetic history of the faults. As part of the effort to understand the diagenetic processes within these fault zones, we have studied analogous processes of rapid carbonate precipitation (scaling) in petroleum reservoir tubing and manmade tunnels. From this, we have identified geochemical signatures in carbonate that characterize rapid CO2 degassing. These data provide constraints for finite element models that predict fluid pressures, multiphase flow patterns, rates and patterns of deformation, subsurface temperatures and heat flow, and geochemistry associated with large fault systems.

  9. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2015-07-14

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  10. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  11. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2011-06-07

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  12. Hydrogen behavior in ice condenser containments

    SciTech Connect (OSTI)

    Lundstroem, P.; Hongisto, O.; Theofanous, T.G.

    1995-09-01

    A new hydrogen management strategy is being developed for the Loviisa ice condenser containment. The strategy relies on containment-wide natural circulations that develop, once the ice condenser doors are forced open, to effectively produce a well-mixed behavior, and a correspondingly slow rise in hydrogen concentration. Levels can then be kept low by a distributed catalytic recombiner system, and (perhaps) an igniter system as a backup, while the associated energy releases can be effectively dissipated in the ice bed. Verification and fine-tuning of the approach is carried out experimentally in the VICTORIA facility and by associated scaling/modelling studies. VICTORIA represents an 1/15th scale model of the Loviisa containment, hydrogen is simulated by helium, and local concentration measurements are obtained by a newly developed instrument specifically for this purpose, called SPARTA. This paper is focused on experimental results from several key experiments that provide a first delineation of key behaviors.

  13. Section 110

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    3 The Optical Properties of Tropical Cirrus Clouds in the Maritime Continent Thunderstorm Experiment from Lidar and Infrared Radiometer Retrievals C.M.R. Platt, S. A. Young, R. T. Austin, G. R. Patterson and S. C. Marsden CSIRO, Division of Atmospheric Research Aspendale, Victoria, Australia Introduction Observations of the optical and structural properties of equa- torial cirrus clouds were made previously in the Atmospheric Radiation Measurement (ARM) Program's Pilot Radiation Observation

  14. North American Energy Ministers Take Further Action on Energy Security and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the Environment | Department of Energy Take Further Action on Energy Security and the Environment North American Energy Ministers Take Further Action on Energy Security and the Environment July 23, 2007 - 2:55pm Addthis Joint Communiqué VICTORIA - Energy ministers for Canada, Mexico and the United States took another step toward enhancing North American energy security and environmental protection, announcing concrete actions on energy science and technology, energy efficiency, deployment

  15. News Item

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0, 2016 Time: 11:00 am Speaker: Frank van Veggel, University of Victoria Title: On the Optical and Magnetic Properties of Ln3+ Based Nanoparticles for Bio-Imaging Location: 67-3111 Chemla Room Abstract: One of our main research thrusts in the field of colloidal nanomaterials and their applications is based on the trivalent lanthanide ions, which have unique optical and magnetic properties. We cover the range from synthesis and characterisation to (in vivo) applications, with a recent focus on

  16. Schneider Electric Boasts Ten Facilities Certified to Superior Energy Performance in North America

    Broader source: Energy.gov [DOE]

    Schneider Electric has doubled the number of sites earning SEP certification since announcing their first five SEP-certified facilities in October 2014. The company’s most recent SEP certifications include two Platinum level facilities in Victoria, British Columbia, Canada and Clovis, California, which improved their energy performance by more than 15% over three years. Facilities in Peru, Indiana and Columbia, South Carolina earned Gold level certification, while the Rojo Gomez, Mexico location earned Silver level certification.

  17. Evolution of the design of a silicon tracker for the Linear Collider

    SciTech Connect (OSTI)

    Cooper, W.E.

    2005-10-01

    A design for the silicon tracker for SiD was proposed at the Victoria Linear Collider Workshop [1]. This paper describes development of that design by the SiD group into a baseline model for simulation studies. The design has been modified to take into account detector fabrication and servicing requirements, features specific to the vertex chamber, and detector elements in the region surrounding the silicon tracker.

  18. Microsoft Word - P04-00147.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ER/ARM-0401 Tropical Warm Pool International Cloud Experiment (TWP-ICE): Cloud and Rain Characteristics in the Australian Monsoon May 2004 P. T. May and C. Jakob Bureau of Meteorology Research Centre Victoria 3001, Australia J. H. Mather Pacific Northwest National Laboratory Richland, Washington Work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research DOE/ER/ARM-0401 iii SUMMARY The impact of oceanic convection on its environment and the

  19. 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Preliminary Investigations of the Role of Smoke Aerosols on Carbon Uptake of a Northern Australian Tropical Savanna K.D. Kanniah, N. Tapper, J Beringer, and X. Zhu School of Geography and Environmental Science Monash University Clayton, Victoria, 3800, Australia C. Long Atmospheric Radiation Measurement Programme Pacific Northwest National Laboratory Richland, Washington L. Hutley Tropical Savanna CRC and School of Science and Primary Industries Charles Darwin University Darwin, Australia

  20. Remotely Controlled, Continuous Observations of Infrared Radiance with the CSIRO/ARM Mark II Radiometer at the SGP CART Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Remotely Controlled, Continuous Observations of Infrared Radiance with the CSIRO/ARM Mark II Radiometer at the SGP CART Site C. M. R. Platt and R. T. Austin Department of Atmospheric Science Colorado State University Fort Collins, Colorado C. M. R. Platt and J. A. Bennett Commonwealth Scientific and Industrial Research Organization Atmospheric Research Aspendale, Victoria, Australia Abstract The Commonwealth Scientific and Industrial Research Organization/Atmospheric Radiation Measurement

  1. Posters

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    7 Posters Observations of Tropical Cirrus Properties in the Pilot Radiation Observation Experiment Using Lidar and the CSIRO ARM Filter Radiometer C.M.R. Platt, S. A. Young, P. J. Manson, and G. R. Patterson CSIRO Division of Atmospheric Research Station Street, Aspendale, Victoria, Australia A narrow beam fast filter radiometer has been developed for the Atmospheric Radiation Measurement (ARM) Program. The radiometer is intended to operate alongside a lidar at ARM sites in a lidar/radiometer

  2. Section 87

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9 Figure 1. The Tiwi Islands (Melville and Bathurst) lie off Australia's northern coast. The instruments were situated at Pularumpi, on the west coast of Melville Island. Retrieval of Tropical Cirrus Cloud Properties from Ground- Based Lidar and Millimeter-Wave Radar Sensing at the Maritime Continent Thunderstorm Experiment R. T. Austin, S. A. Young, C.M.R. Platt and G. R. Patterson CSIRO, Division of Atmospheric Research Aspendale, Victoria, Australia S. M. Sekelsky and R. E. McIntosh Microwave

  3. Cloud and Precipitation Fields Around Darwin in the Transition Season

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Precipitation Fields Around Darwin in the Transition Season P. T. May Bureau of Meteorology Research Centre Melbourne, 3001, Victoria, Australia Introduction An interesting, and very relevant question, for the Atmospheric Radiation Measurement (ARM) Program is how cloud characteristics and their seasonal and diurnal variation changes across the tropics. In particular, how does he cloud field around the new SRCS site compare with nearby regions. Thus, the aim of this study is to look at the

  4. Fission-track tectonic studies of the Transantarctic Mountains, Beardmore Glacier area

    SciTech Connect (OSTI)

    Fitzgerald, P.G.

    1986-01-01

    The Transantarctic Mountains are a major transcontinental range stretching for some 4000 kilometers, varying from 200-400 kilometers in width, and having elevations up to 4500 meters. The uplift and formation of the Transantarctic Mountains have always been something of an enigma, but recent apatite fission-track analysis is providing important new information not only about their uplift history but also about the implications of that uplift history for the glacial history of Antarctica as a whole. The main field objective of this project was to collect samples for fission-track analysis to determine the timing and rate of uplift of the Transantarctic Mountains and measure relative vertical displacements across faults within the range. Results from southern Victoria Land indicate that uplift of the Transantarctic Mountains was initiated at about 50 million years ago and since that time the mountains have undergone some 5 kilometers of uplift at an average rate of 100 meters per million years. It is important to realize, however, that this is an average rate and may well conceal pulses of faster and slower uplift or even periods of subsidence. The amount of uplift across the mountain range is differential; from the axis of maximum uplift about 30 kilometers inland of the Victoria Land coast, the mountains dip gently westward under the polar ice cap. The study was extended to the Beardmore Glacier area to see whether the uplift history and tectonics varies from that observed in southern Victoria Land.

  5. Evaluation and demonstration of methods for improved fuel utilization. First semi-annual progress report, September 1979-March 1980

    SciTech Connect (OSTI)

    Decher, U.

    1980-06-25

    Demonstrations of improved fuel management and burnup are being performed in the Fort Calhoun reactor. More efficient fuel management will be achieved through the implementation of a low leakage concept called SAVFUEL (Shimmed And Very Flexible Uranium Element Loading), which is expected to reduce uranium requirements by 2 to 4%. The burnup will be increased sufficiently to reduce uranium requirements by 5 to 15%. Four fuel assemblies scheduled to demonstrate the SAVFUEL duty cycle and loaded into the core in December 1978 were inspected visually prior to their second exposure cycle. In addition, seventeen fuel assemblies were inspected after their fourth exposure cycle having achieved assembly average burnup up to 36 GWD/T. One assembly has been reinserted into Cycle 6 for a fifth exposure cycle. The preliminary results of all visual fuel inspections which appear to show excellent fuel rod performance are presented in this report. This report also contains the results of a licensing activity which was performed to allow insertion of a highly burned assembly into the reactor for a fifth irradiation cycle.

  6. Rsdometty C.M.R. Platt, J. W. Bennett, S. A. Young, M. D. Fenwick, P. J. Manson, G. R. Patterson, and B. Petraitis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Rsdometty C.M.R. Platt, J. W. Bennett, S. A. Young, M. D. Fenwick, P. J. Manson, G. R. Patterson, and B. Petraitis CSIRO Division of Atmospheric Research Station Street Aspendale, Victoria, Australia Introduction a suitable detector, such a radiometer can yield a response time of about one second, which will follow most cl6ud fluctuations with minimum lag. The LIRAD Method The use of narrow beam filter radiometers with lidars goes back some considerable time. The CSIRO Marl< I radiometer was

  7. Summary report for subcontract No. 0412J0004-3Y, task order 23. Final report

    SciTech Connect (OSTI)

    1997-10-02

    This task covers work performed by Carlton S. Young for various projects in Group P-22, Los Alamos National Lab from September 1996 to September 22, 1997. Projects worked on and charged to contract: (1) rebound, 80%; (2) reports on Nevada events Divider and Victoria, 10%; (3) FORTRAN modifications to a Macintosh version of the ACCEPT code and calculations on GAAs PCDs and Cherenkov detectors, 0%; (4) design of time and space-resolved burn measurements for the National Ignition Facility (NIF) based on a gas Cherenkov gamma-ray detector and proposals for more money to do the same, 10%. A summary of work on each project is included.

  8. Design report on SCDAP/RELAP5 model improvements - debris bed and molten pool behavior

    SciTech Connect (OSTI)

    Allison, C.M.; Rempe, J.L.; Chavez, S.A.

    1994-11-01

    the SCDAP/RELAP5/MOD3 computer code is designed to describe the overall reactor coolant system thermal-hydraulic response, core damage progression, and in combination with VICTORIA, fission product release and transport during severe accidents. Improvements for existing debris bed and molten pool models in the SCDAP/RELAP5/MOD3.1 code are described in this report. Model improvements to address (a) debris bed formation, heating, and melting; (b) molten pool formation and growth; and (c) molten pool crust failure are discussed. Relevant data, existing models, proposed modeling changes, and the anticipated impact of the changes are discussed. Recommendations for the assessment of improved models are provided.

  9. PowerPoint Presentation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    RapiDx Victoria VanderNoot, Ph.D. February 25, 2015 Sandia MedTech Showcase Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2015-1392C Brief Technology Overview - RapiDx Rapid, Automated Point-of-Care System (RapiDx) * Portable microfluidic in vitro diagnostic instrument

  10. Section 61

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Retrieval of Cloud Properties from Ground-Based Lidar/Radiometer Data C.M.R. Platt, S.A. Young, R.T. Austin, S.C. Marsden, G.R. Patterson, J.A. Bennett, B. Petraitis, and B. Turner CSIRO, Division of Atmospheric Research Aspendale, Victoria, Australia Abstract The retrieval of cloud properties from Lidar/Radiometer data by the LIRAD method is described. Several data sets taken by the CSIRO Division of Atmospheric Research are now available for such retrievals as a result of several major field

  11. Correlation between precision gravity and subsidence measurements at Cerro Prieto

    SciTech Connect (OSTI)

    Zelwer, R.; Grannell, R.B.

    1982-10-01

    Precision gravity measurements were made in the region of the Cerro Prieto geothermal field at yearly intervals from 1977 to 1981 to assess the feasibility of using gravity to determine subsurface reservoir changes with time. The extent of mass recharge in response to the continued production of fluids from this field was studied. Changes in gravity and ground elevation were observed throughout the region for the period of observation. Results indicate that the largest changes observed were the result of the Magnitude 6.1 (Caltech) Victoria earthquake of 8 June 1980. The epicenter of this earthquake was located 25 km southeast of the field on the Cerro Prieto Fault, which bounds the field on the southwest. Subsidence of up to 55 cm was measured east of the power plant, in the region between the northern end of the Cerro Prieto Fault and the southern end of the Imperial Fault. This area has been postulated to be the site of an active spreading center or pull-apart basin, and has been characterized by a high level of seismic activity during the last 10 years. Minor subsidence and small related gravity changes for the period preceeding the Victoria earthquake suggest that in spite of large fluid production rates, the reservoir is being almost completely recharged and that a measurable increase in subsurface density may be taking place. The results of measurements of horizontal ground motions made in this area are discussed in relation to the gravity and subsidence observations.

  12. Barwood CNG Cab Fleet Study: Final Results

    SciTech Connect (OSTI)

    Whalen, P.; Kelly, K.; John, M.

    1999-05-03

    This report describes a fleet study conducted over a 12-month period to evaluate the operation of dedicated compress natural gas (CNG) Ford Crown Victoria sedans in a taxicab fleet. In the study, we assess the performance and reliability of the vehicles and the cost of operating the CNG vehicles compared to gasoline vehicles. The study results reveal that the CNG vehicles operated by this fleet offer both economic and environmental advantages. The total operating costs of the CNG vehicles were about 25% lower than those of the gasoline vehicles. The CNG vehicles performed as well as the gasoline vehicles, and were just as reliable. Barwood representatives and drivers have come to consider the CNG vehicles an asset to their business and to the air quality of the local community.

  13. Oil and gas developments in South Africa, Central America, Caribbean Area, and Mexico in 1984

    SciTech Connect (OSTI)

    Tappmeyer, D.M.

    1985-10-01

    For the 25 countries described in the region, exploration activity continued to be centered in proven petroleum provinces in 1984. Exploration activity increased in Brazil, Colombia, and Venezuela. Important, successful exploration efforts continued in areas around Cano Limon field in Colombia, and Guafita and La Victoria fields in Venezuela. Notable discoveries in South America included the 42-46-1X San Martin Cretaceous discovery by Shell in the Ucayali basin in Peru, the significant oil and gas discovery at Palmar Largo ES-1 by YPF in Argentina, and the first offshore gas discovery, the Pecten 1-SPS-20 wildcat in the Santo basin in Brazil. Oil production increased in Barbados, Brazil, Colombia, Ecuador, Mexico, Peru, Trinidad and Tobago, and Venezuela. A notable increase (40%) in oil production occurred in Brazil. Gas production increased in Argentina, Barbados, Colombia, Peru, and Trinidad and Tobago. 8 figures, 8 tables.

  14. New pipe-lay method proposed for under water

    SciTech Connect (OSTI)

    Not Available

    1980-03-31

    The ''ice-hole bottom pull'' technique of pipelaying, developed by Polar Gas Ltd. for the laying of pipe across M'Clure Strait between Melville and Victoria Islands, Can., since the ice at some points is too thick to allow installation of a continuous trench, is described in detail, including the drilling of holes 2 km apart and insulating them to prevent refreezing; pulling the pipe from hole to hole via a series of increasingly heavy cables; undersea welding of the pipe after it is pulled into place; and the need to lay pipe in a tunnel at least 45 m below the sea bottom near shore and in other areas subject to ice scour, where the pipe could be damaged by exceptionally thick ice.

  15. Development of a simple 5-15 litre per hour LNG refueling system

    SciTech Connect (OSTI)

    Corless, A.J.; Sarangi, S.; Hall, J.L.; Barclay, J.A.

    1994-12-31

    A variable capacity, small-scale liquefied natural gas (LNG) refueling system has been designed, built, and tested at the Cryofuel Systems` Laboratory, University of Victoria, Canada. The system, designed to continuously liquefy between 5 and 15 litres of NG, utilizes liquid nitrogen (LN{sub 2}) as its cold source and contains most of the components found in a typical commercial refueling system; i.e. purification system, liquefier, LNG storage, automatic control and monitoring system. This paper describes the design of the system as well as the results of a set of LNG production trials. The performance of the system exceeded expected LNG production rates, but at levels of efficiency somewhat less than predicted. Cryofuel Systems expects to use this system to implement an LNG vehicle demonstration program and to gain experience in the integration of LNG refueling systems which exploit advanced liquefaction technology such as magnetic refrigeration.

  16. Arsenic microdistribution and speciation in toenail clippings of children living in a historic gold mining area

    SciTech Connect (OSTI)

    Pearce, Dora C.; Dowling, Kim; Gerson, Andrea R.; Sim, Malcolm R.; Sutton, Stephen R.; Newville, Matthew; Russell, Robert; McOrist, Gordon

    2010-05-04

    Arsenic is naturally associated with gold mineralization and elevated in some soils and mine waste around historical gold mining activity in Victoria, Australia. To explore uptake, arsenic concentrations in children's toenail clippings and household soils were measured, and the microdistribution and speciation of arsenic in situ in toenail clipping thin sections investigated using synchrotron-based X-ray microprobe techniques. The ability to differentiate exogenous arsenic was explored by investigating surface contamination on cleaned clippings using depth profiling, and direct diffusion of arsenic into incubated clippings. Total arsenic concentrations ranged from 0.15 to 2.1 {micro}g/g (n = 29) in clipping samples and from 3.3 to 130 {micro}g/g (n = 22) in household soils, with significant correlation between transformed arsenic concentrations (Pearson's r = 0.42, P = 0.023) when household soil was treated as independent. In clipping thin sections (n = 2), X-ray fluorescence (XRF) mapping showed discrete layering of arsenic consistent with nail structure, and irregular arsenic incorporation along the nail growth axis. Arsenic concentrations were heterogeneous at 10 x 10 {micro}m microprobe spot locations investigated (< 0.1 to 13.3 {micro}g/g). X-ray absorption near-edge structure (XANES) spectra suggested the presence of two distinct arsenic species: a lower oxidation state species, possibly with mixed sulphur and methyl coordination (denoted As{sub (-S, -ch3)}{sup {approx}III}); and a higher oxidation state species (denoted As{sub (-O)}{sup {approx}V}). Depth profiling suggested that surface contamination was unlikely (n = 4), and XRF and XANES analyses of thin sections of clippings incubated in dry or wet mine waste, or untreated, suggested direct diffusion of arsenic occurred under moist conditions. These findings suggest that arsenic in soil contributes to some systemic absorption associated with periodic exposures among children resident in areas of historic

  17. Optimization of Sparse Matrix-Vector Multiplication on Emerging Multicore Platforms

    SciTech Connect (OSTI)

    Williams, Samuel; Oliker, Leonid; Vuduc, Richard; Shalf, John; Yelick, Katherine; Demmel, James

    2008-10-16

    We are witnessing a dramatic change in computer architecture due to the multicore paradigm shift, as every electronic device from cell phones to supercomputers confronts parallelism of unprecedented scale. To fully unleash the potential of these systems, the HPC community must develop multicore specific-optimization methodologies for important scientific computations. In this work, we examine sparse matrix-vector multiply (SpMV) - one of the most heavily used kernels in scientific computing - across a broad spectrum of multicore designs. Our experimental platform includes the homogeneous AMD quad-core, AMD dual-core, and Intel quad-core designs, the heterogeneous STI Cell, as well as one of the first scientific studies of the highly multithreaded Sun Victoria Falls (a Niagara2 SMP). We present several optimization strategies especially effective for the multicore environment, and demonstrate significant performance improvements compared to existing state-of-the-art serial and parallel SpMV implementations. Additionally, we present key insights into the architectural trade-offs of leading multicore design strategies, in the context of demanding memory-bound numerical algorithms.

  18. PERI - Auto-tuning Memory Intensive Kernels for Multicore

    SciTech Connect (OSTI)

    Bailey, David H; Williams, Samuel; Datta, Kaushik; Carter, Jonathan; Oliker, Leonid; Shalf, John; Yelick, Katherine; Bailey, David H

    2008-06-24

    We present an auto-tuning approach to optimize application performance on emerging multicore architectures. The methodology extends the idea of search-based performance optimizations, popular in linear algebra and FFT libraries, to application-specific computational kernels. Our work applies this strategy to Sparse Matrix Vector Multiplication (SpMV), the explicit heat equation PDE on a regular grid (Stencil), and a lattice Boltzmann application (LBMHD). We explore one of the broadest sets of multicore architectures in the HPC literature, including the Intel Xeon Clovertown, AMD Opteron Barcelona, Sun Victoria Falls, and the Sony-Toshiba-IBM (STI) Cell. Rather than hand-tuning each kernel for each system, we develop a code generator for each kernel that allows us to identify a highly optimized version for each platform, while amortizing the human programming effort. Results show that our auto-tuned kernel applications often achieve a better than 4X improvement compared with the original code. Additionally, we analyze a Roofline performance model for each platform to reveal hardware bottlenecks and software challenges for future multicore systems and applications.

  19. Hazelwood 2030

    SciTech Connect (OSTI)

    Rich, G.; Hayes, B.; Heinz, G.

    2007-12-15

    Funding made available by both the Victoria State and Australian Federal Governments for the demonstration of low emission technologies is enabling the implementation of pilot brown coal drying technology at unit 1 of the International Power managed Hazelwood power station. This will dry the run of mine brown coal from above 60% moisture content to approximately 12% moisture content in an initial 50% feed train. It is the core component of a major upgrade programme, Hazelwood 2030 described in this article. Key features of the WTA and retrofit project can be summarised as follow: Unit 1: Installation of new turbines. Steam extraction for WTA supplying 23 kg/s. Unit output increased to between 215 and 217 MWe, with the same steam flow as the original design; Installation of boiler feed water heaters providing 3% improvement in unit boiler efficiency; Installation of a WTA fluidized bed drier sufficient to provide the fuel for 50% of the thermal heat input required for 50% cocombusiton of dried brown coal (DBC); Conversion of boiler and adaptation of the firing system to 50% co-combustion of dried brown coal and ROM brown coal; Implementation outage scheduled for 4Q, 2009; Unit 2: Installation of new turbines, unit output increased to 220 MWe, again with the same steam flow as the original design; Installation of boiler feed water heaters providing 5% improvement on unit boiler efficiency; Implementation outage scheduled for 2Q 2009. 11 figs.

  20. Temporal Changes in Gene Expression in Rainbow Trout Exposed to Ethynyl Estradiol

    SciTech Connect (OSTI)

    Hook, Sharon E.; Skillman, Ann D.; Small, Jack A.; Schultz, Irv R.

    2006-11-25

    We examined changes in the genomic response during continuous exposure to the xenoestrogen ethynylestradiol. Isogenic rainbow trout Onorhyncus mykiss were exposed to nominal concentrations of 100 ng/L ethynyl estradiol (EE2) for a period 3 weeks. At fixed time points within the exposure fish were euthanized, livers harvested and RNA extracted. Fluorescently labeled cDNA were generated and hybridized against a commercially available Salmonid array (GRASP project, University of Victoria) spotted with 16,000 cDNA's. The slides were scanned to measure abundance of a given transcript in each sample relative to controls. Data were analyzed via Genespring (Silicon Genetics) to identify a list of up and down regulated genes, and to determine gene clustering patterns that can be used as ''expression signatures''. Gene ontology was determined using the annotation available from the GRASP website. Our analysis indicates each exposure time period generated specific gene expression profiles. Changes in gene expression were best understood by grouping genes by their gene expression profiles rather than examining fold change at a particular time point. Many of the genes commonly used as biomarkers of exposure to xenoestrogens were not induced initially and did not have gene expression profiles typical of the majority of genes with altered expression.

  1. Living in the Nuclear Age: Families and the threat of nuclear war

    SciTech Connect (OSTI)

    Demorest, D.M.

    1991-01-01

    The main interest of this research was to add to the body of knowledge about the possible psychological impact of the nuclear threat on the family unit. Data were utilized from the Family Interaction, Stress and Nuclear War study conducted by Jules Riskin, M.D. and Victoria Dickerson, Ph.D. at the Mental Research Institute in Palo Alto, California. The sample consisted of ten families who were recruited for this study. In order to examine family-interaction variables and the impact of the threat of nuclear war, a standardized semi-structured family interview was conducted. Topics ranged from ordinary activities to external, non-nuclear stresses such as landslides or hurricanes, to the topic of nuclear war. A distinction is drawn between a family's level of nuclear concern while they discuss nuclear issues and a family's level of nuclear concern when viewed in the context of their overall pattern of family communication. In terms of family coping, family nuclear concern was found to be significantly related to two family-coping strategies. Families who utilized the coping strategies of seeking spiritual support and mobilizing the family to acquire and accept help were significantly less concerned about the threat of nuclear war.

  2. Structure of the Newcastle disease virus F protein in the post-fusion conformation

    SciTech Connect (OSTI)

    Swanson, Kurt; Wen, Xiaolin; Leser, George P.; Paterson, Reay G.; Lamb, Robert A.; Jardetzky, Theodore S. (Stanford-MED); (NWU); (HHMI)

    2010-11-17

    The paramyxovirus F protein is a class I viral membrane fusion protein which undergoes a significant refolding transition during virus entry. Previous studies of the Newcastle disease virus, human parainfluenza virus 3 and parainfluenza virus 5 F proteins revealed differences in the pre- and post-fusion structures. The NDV Queensland (Q) F structure lacked structural elements observed in the other two structures, which are key to the refolding and fusogenic activity of F. Here we present the NDV Australia-Victoria (AV) F protein post-fusion structure and provide EM evidence for its folding to a pre-fusion form. The NDV AV F structure contains heptad repeat elements missing in the previous NDV Q F structure, forming a post-fusion six-helix bundle (6HB) similar to the post-fusion hPIV3 F structure. Electrostatic and temperature factor analysis of the F structures points to regions of these proteins that may be functionally important in their membrane fusion activity.

  3. Optimization of a Lattice Boltzmann Computation on State-of-the-Art Multicore Platforms

    SciTech Connect (OSTI)

    Williams, Samuel; Carter, Jonathan; Oliker, Leonid; Shalf, John; Yelick, Katherine

    2009-04-10

    We present an auto-tuning approach to optimize application performance on emerging multicore architectures. The methodology extends the idea of search-based performance optimizations, popular in linear algebra and FFT libraries, to application-specific computational kernels. Our work applies this strategy to a lattice Boltzmann application (LBMHD) that historically has made poor use of scalar microprocessors due to its complex data structures and memory access patterns. We explore one of the broadest sets of multicore architectures in the HPC literature, including the Intel Xeon E5345 (Clovertown), AMD Opteron 2214 (Santa Rosa), AMD Opteron 2356 (Barcelona), Sun T5140 T2+ (Victoria Falls), as well as a QS20 IBM Cell Blade. Rather than hand-tuning LBMHD for each system, we develop a code generator that allows us to identify a highly optimized version for each platform, while amortizing the human programming effort. Results show that our auto-tuned LBMHD application achieves up to a 15x improvement compared with the original code at a given concurrency. Additionally, we present detailed analysis of each optimization, which reveal surprising hardware bottlenecks and software challenges for future multicore systems and applications.

  4. Formulating a VET roadmap for the waste and recycling sector: A case study from Queensland, Australia

    SciTech Connect (OSTI)

    Davis, G.

    2012-10-15

    Highlights: Black-Right-Pointing-Pointer Existing qualifications do not meet the needs of the sector in Queensland. Black-Right-Pointing-Pointer Businesses may not be best positioned to identify training needs. Black-Right-Pointing-Pointer Companies are developing training internally to meet their own specific needs. Black-Right-Pointing-Pointer Smaller companies lack the resources to develop internal training are disadvantaged. Black-Right-Pointing-Pointer There is industry support for an entry-level, minimum industry qualification. - Abstract: Vocational Education and Training (VET) is an essential tool for providing waste management and recycling workers with the necessary skills and knowledge needed to beneficially influence their own employment and career development; and to also ensure productivity and safe working conditions within the organisations in which they are employed. Current training opportunities within Queensland for the sector are limited and not widely communicated or marketed; with other States, particularly Victoria and New South Wales, realising higher numbers of VET enrollments for waste management courses. This paper presents current VET opportunities and trends for the Queensland waste management sector. Results from a facilitated workshop to identify workforce requirements and future training needs organised by the Waste Contractors and Recyclers Association of Queensland (WCRAQ) are also presented and discussion follows on the future training needs of the industry within Queensland.

  5. Simion 3D Version 6.0 User`s Manual

    SciTech Connect (OSTI)

    Dahl, D.A.

    1995-11-01

    The original SIMION was an electrostatic lens analysis and design program developed by D.C. McGilvery at Latrobe University, Bundoora Victoria, Australia, 1977. SIMION for the PC, developed at the Idaho National Engineering Laboratory, shares little more than its name with the original McGilvery version. INEL`s fifth major SIMION release, version 6.0, represents a quantum improvement over previous versions. This C based program can model complex problems using an ion optics workbench that can hold up to 200 2D and/or 3D electrostatic/magnetic potential arrays. Arrays can have up to 10,000,000 points. SIMION 3D`s 32 bit virtual Graphics User Interface provides a highly interactive advanced user environment. All potential arrays are visualized as 3D objects that the user can cut away to inspect ion trajectories and potential energy surfaces. User programs have been greatly extended in versatility and power. A new geometry file option supports the definition of highly complex array geometry. Extensive algorithm modifications have dramatically improved this version`s computational speed and accuracy.

  6. Comparative Genome Structure, Secondary Metabolite, and Effector Coding Capacity across Cochliobolus Pathogens

    SciTech Connect (OSTI)

    Condon, Bradford J.; Leng, Yueqiang; Wu, Dongliang; Bushley, Kathryn E.; Ohm, Robin A.; Otillar, Robert; Martin, Joel; Schackwitz, Wendy; Grimwood, Jane; MohdZainudin, NurAinlzzati; Xue, Chunsheng; Wang, Rui; Manning, Viola A.; Dhillon, Braham; Tu, Zheng Jin; Steffenson, Brian J.; Salamov, Asaf; Sun, Hui; Lowry, Steve; LaButti, Kurt; Han, James; Copeland, Alex; Lindquist, Erika; Barry, Kerrie; Schmutz, Jeremy; Baker, Scott E.; Ciuffetti, Lynda M.; Grigoriev, Igor V.; Zhong, Shaobin; Turgeon, B. Gillian

    2013-01-24

    The genomes of five Cochliobolus heterostrophus strains, two Cochliobolus sativus strains, three additional Cochliobolus species (Cochliobolus victoriae, Cochliobolus carbonum, Cochliobolus miyabeanus), and closely related Setosphaeria turcica were sequenced at the Joint Genome Institute (JGI). The datasets were used to identify SNPs between strains and species, unique genomic regions, core secondary metabolism genes, and small secreted protein (SSP) candidate effector encoding genes with a view towards pinpointing structural elements and gene content associated with specificity of these closely related fungi to different cereal hosts. Whole-genome alignment shows that three to five of each genome differs between strains of the same species, while a quarter of each genome differs between species. On average, SNP counts among field isolates of the same C. heterostrophus species are more than 25 higher than those between inbred lines and 50 lower than SNPs between Cochliobolus species. The suites of nonribosomal peptide synthetase (NRPS), polyketide synthase (PKS), and SSP encoding genes are astoundingly diverse among species but remarkably conserved among isolates of the same species, whether inbred or field strains, except for defining examples that map to unique genomic regions. Functional analysis of several strain-unique PKSs and NRPSs reveal a strong correlation with a role in virulence.

  7. Exploration for stratigraphic traps in a foreland basin using a sequence stratigraphic simulation: Examples from the Eocene/Oligocene of the Apure-Llanos basin, Venezuela

    SciTech Connect (OSTI)

    Reistroffer, J.; Levine, P.A.; Kendall, C.G.; Finno, A.

    1996-12-31

    Foreland basin depositional sequences provide a sensitive record of the interaction between tectonism, eustatic sea level fluctuations, and sedimentation rates. Interplay between these controlling factors creates sedimentary geometries which are unique to this tectonic setting and form excellent stratigraphic hydrocarbon traps. Incised valley fill deposits, {open_quote}forced regression{close_quote} deposits, and combination structure-stratigraphic traps are the predominant reservoir types. In an effort to extend our understanding of the development of these traps, the sequence stratigraphy of a regional seismic transact through the Apure-Llanos basin was simulated. From the Late Eocene through Oligocene, the Apure-Llanos basin was Characterized by multiple phases of compression and a southeast migrating depocenter. Sands of the Mirador and Carbonera formations, which onlap the Arauca Arch to the southeast, were shed from the Guyana craton and were Cannibalized from sediments along the deformation front to the northwest. These sands comprise the principal reservoirs in the study area. Shales of the Leon Formation, which act as a regional seal, were deposited during rapid flexural subsidence and eustatic sea level rise during the early Oligocene. The Arauca Arch acted as a focal mechanism for east and southeast migrating hydrocarbons. Simulation results predict an important stratigraphic pinchout of the Mirador Formation sands against the Arauca Arch, which correlates with the Arauca Reid in Colombia to the southwest. Also, modeling indicates that minimal Tertiary oil production In the La Victoria Field to the east is due to the lack of an adequate seal. Our results provide a conceptual model which predicts hydrocarbon reservoir and seal relationships in a foreland basin setting with limited data control.

  8. Ultrastructural and Single-Cell-Level Characterization Reveals Metabolic Versatility in a Microbial Eukaryote Community from an Ice-Covered Antarctic Lake

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Wei; Podar, Mircea; Morgan-Kiss, Rachael M.

    2016-04-15

    The McMurdo Dry Valleys (MCM) of southern Victoria Land, Antarctica, harbor numerous ice-covered bodies of water that provide year-round liquid water oases for isolated food webs dominated by the microbial loop. Single-cell microbial eukaryotes (protists) occupy major trophic positions within this truncated food web, ranging from primary producers (e.g., chlorophytes, haptophytes, and cryptophytes) to tertiary predators (e.g., ciliates, dinoflagellates, and choanoflagellates). To advance the understanding of MCM protist ecology and the roles of MCM protists in nutrient and energy cycling, we investigated potential metabolic strategies and microbial interactions of key MCM protists isolated from a well-described lake (Lake Bonney). Fluorescence-activatedmore » cell sorting (FACS) of enrichment cultures, combined with single amplified genome/amplicon sequencing and fluorescence microscopy, revealed that MCM protists possess diverse potential metabolic capabilities and interactions. Two metabolically distinct bacterial clades (FlavobacteriaandMethylobacteriaceae) were independently associated with two key MCM lake microalgae (IsochrysisandChlamydomonas, respectively). We also report on the discovery of two heterotrophic nanoflagellates belonging to the Stramenopila supergroup, one of which lives as a parasite ofChlamydomonas, a dominate primary producer in the shallow, nutrient-poor layers of the lake. Single-cell eukaryotes called protists play critical roles in the cycling of organic matter in aquatic environments. In the ice-covered lakes of Antarctica, protists play key roles in the aquatic food web, providing the majority of organic carbon to the rest of the food web (photosynthetic protists) and acting as the major consumers at the top of the food web (predatory protists). In this study, we utilized a combination of techniques (microscopy, cell sorting, and genomic analysis) to describe the trophic abilities of Antarctic lake protists and their potential

  9. Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine

    SciTech Connect (OSTI)

    Pletnev, Vladimir Z. Pletneva, Nadya V.; Lukyanov, Konstantin A.; Souslova, Ekaterina A.; Fradkov, Arkady F.; Chudakov, Dmitry M.; Chepurnykh, Tatyana; Yampolsky, Ilia V.; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei

    2013-09-01

    The crystal structure of the novel red emitting fluorescent protein from lancelet Branchiostoma lanceolatum (Chordata) revealed an unusual five residues cyclic unit comprising Gly58-Tyr59-Gly60 chromophore, the following Phe61 and Tyr62 covalently bound to chromophore Tyr59. A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the ‘core’ structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (λ{sub ex}/λ{sub em} = 502/511 nm) and red laRFP (λ{sub ex}/λ{sub em} ≃ 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-ΔS83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ∼20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C{sup β} atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (

  10. Single-well experimental design for studying residual trapping of superciritcal carbon dioxide

    SciTech Connect (OSTI)

    Zhang, Y.; Freifeld, B.; Finsterle, S.; Leahy, M.; Ennis-King, J.; Paterson, L.; Dance, T.

    2010-06-15

    The objective of our research is to design a single-well injection-withdrawal test to evaluate residual phase trapping at potential CO{sub 2} geological storage sites. Given the significant depths targeted for CO{sub 2} storage and the resulting high costs associated with drilling to those depths, it is attractive to develop a single-well test that can provide data to assess reservoir properties and reduce uncertainties in the appraisal phase of site investigation. The main challenges in a single-well test design include (1) difficulty in quantifying the amount of CO{sub 2} that has dissolved into brine or migrated away from the borehole; (2) non-uniqueness and uncertainty in the estimate of the residual gas saturation (S{sub gr}) due to correlations among various parameters; and (3) the potential biased S{sub gr} estimate due to unaccounted heterogeneity of the geological medium. To address each of these challenges, we propose (1) to use a physical-based model to simulation test sequence and inverse modeling to analyze data information content and to quantify uncertainty; (2) to jointly use multiple data types generated from different kinds of tests to constrain the Sgr estimate; and (3) to reduce the sensitivity of the designed tests to geological heterogeneity by conducting the same test sequence in both a water-saturated system and a system with residual gas saturation. To perform the design calculation, we build a synthetic model and conduct a formal analysis for sensitivity and uncertain quantification. Both parametric uncertainty and geological uncertainty are considered in the analysis. Results show (1) uncertainty in the estimation of Sgr can be reduced by jointly using multiple data types and repeated tests; and (2) geological uncertainty is essential and needs to be accounted for in the estimation of S{sub gr} and its uncertainty. The proposed methodology is applied to the design of a CO{sub 2} injection test at CO2CRC's Otway Project Site, Victoria

  11. Gene expression patterns in Rainbow Trout, Oncorhynchus mykiss, exposed to a suite of model toxicants

    SciTech Connect (OSTI)

    Hook, Sharon E.; Skillman, Ann D.; Small, Jonathan A.; Schultz, Irv R.

    2006-05-25

    The increased availability and use of DNA microarrays has allowed the characterization of gene expression patterns associated with different toxicants. An important question is whether toxicant induced changes in gene expression in fish are sufficiently diverse to allow for identification of specific modes of action and/or specific contaminants. In theory, each class of toxicant may generate a gene expression profile unique to its mode of toxic action. We exposed isogenic (cloned) rainbow trout Oncorhyncus mykiss, to sublethal levels of a series of model toxicants with varying modes of action, including ethynylestradiol (xeno-estrogen), trenbolone (anabolic steroid; model androgen), 2,2,4,4´tetrabromodiphenyl ether (BDE-47, thyroid active), diquat (oxidant stressor), chromium VI, and benzo[a]pyrene (BaP) for a period of 1-3 weeks. Following exposure, fish were euthanized, livers harvested and RNA extracted. Fluorescently labeled cDNA were generated and hybridized against a commercially available Atlantic Salmon / Trout array (GRASP project, University of Victoria) spotted with 16,000 cDNA’s. The slides were scanned to measure abundance of a given transcript in each sample relative to controls. Data were analyzed via Genespring (Silicon Genetics) to identify a list of up and down regulated genes, as well as to determine gene clustering patterns that can be used as “expression signatures”. Our analysis indicates each toxicant generated specific gene expression profiles. Most genes exhibiting altered expression responded to only one of the toxicants. Relatively few genes are co-expressed in multiple treatments. For example, BaP and Diquat, both of which exert toxicity via oxidative stress, up-regulated 28 of the same genes, of over 100 genes altered by ether treatment. Other genes associated with steroidogenesis, p450 and estrogen responsive genes appear to be useful for selectively identifying toxicant mode of in fish, suggesting a link between gene expression

  12. Zambia : long-term generation expansion study - executive summary.

    SciTech Connect (OSTI)

    Conzelmann, G.; Koritarov, V.; Buehring, W.; Veselka, T.; Decision and Information Sciences

    2008-02-28

    and optimization of hydro cascades, resulting in the better utilization of available hydro potential. Numerous analyses were performed for different stages of system development. These include system configurations that correspond to years 1997, 2001, 2015 and 2020. Additional simulations were performed in order to determine the operational parameters of the three existing hydro power stations Victoria Falls, Kariba, and Kafue Gorge Upper, that correspond to the situation before and after their rehabilitation. The rehabilitation works for these three major power stations, that would bring their operational parameters and availability back to the design level, are planned to be carried out in the period until 2000. The main results of the hydro operations studies are presented in Table ES-1. These results correspond to VALORAGUA simulations of system configurations in the years 2001 and 2015. The minimum, average, and maximum electricity generation is based on the simulation of monthly water inflows that correspond to the chronological series of unregulated water inflows at each hydro profile in the period from April 1961 to March 1990. The recommended hydrology dataset provided in the Hydrology Report of the SADC Energy Project AAA 3.8 was used for this study.