National Library of Energy BETA

Sample records for venezuela regulation induced

  1. THE PEARL FISHERY OF VENEZUELA

    E-Print Network [OSTI]

    THE PEARL FISHERY OF VENEZUELA Marine Biological Lafi'ir-toiy X.I B K. A. R TT JUN 2 41950 WOODS Albert M. Day, Director Special Scientific Report - Fisheries Ho. 26 THE PEARL FISHERY OF VENEZUELA Paul to Venezuela, made travel arrangements, arranged for the cooperation of Venecuelan agencies, and otheri

  2. Cephalopod Resources of Venezuela Introduction

    E-Print Network [OSTI]

    Cephalopod Resources of Venezuela Introduction The cephalopods in the Western Central Atlantic producing nations in the Western Central Atlantic are Mexico and Ven ezuela. While Venezuela has the lead in production of octopus (mainly Octopus maya). In Venezuela, both squid and octopus are produced as a by

  3. LPG in Venezuela

    SciTech Connect (OSTI)

    Romero, O.

    1986-01-01

    The use of LPG for domestic consumption in Venezuela began in late 1929 when LPG was imported in lots of 500 cylinders. These cylinders were then returned to the U.S. for refilling. Total consumption at that time was some 40M/sup 3/ (250 barrels) per year and by 1937 had grown to some 540M/sup 3/ (3,400 barrels) per year. Local production of LPG from gas began in the mid thirties with a small cooling plant in the Mene Grande Field in the Lake Maracaibo area, the first field to produce oil in Venezuela (1914). This plant produced gasoline for a refinery and some of the first LPG used in Venezuela for domestic consumption. The capacity of this plant was insufficient to satisfy the growing demand for LPG which was supplied from refinery production until the development of the natural gas processing industry. At the present time, Venezuelan refineries are net consumers of LPG.

  4. ¿Qué pasa con el teatro de Venezuela?

    E-Print Network [OSTI]

    Castillo, Susana D.

    1973-01-01

    PASA CON EL TEATRO El\\l VENEZUELA? Lü nueva diaiiiatiirgiaRico contó con Cámara de Venezuela, grupo que presentó diuoallí que, a pesar de que Venezuela sea probablemente el país

  5. Un teatro campesino en Venezuela

    E-Print Network [OSTI]

    Korn, Guillermo

    1976-10-01

    102 LATIN AMERICAN THEATRE REVIEW Un teatro campesino en Venezuela GUILLERMO KORN Producto espontáneo de la participación colectiva nació el grupo de teatro del Asentamiento Campesino Sabaneta, en el Estado Aragua de Venezuela. Las obras de su..., despertó interés. En la Universidad Central de Venezuela quieren saber más de ustedes. Para eso he venido. . . ? ¿Podrían eventualmente hacer una representación en la Escuela de Comuni cación Social? Almeida: {Claro que sí! Si nos dan un medio para...

  6. The Price of Political Opposition: Evidence from Venezuela's Maisanta

    E-Print Network [OSTI]

    Hsieh, Chang-Tai; Miguel, Edward; Ortega, Daniel; Rodriguez, Francisco

    2009-01-01

    Spending in Chavez’s Venezuela”, unpublished manuscript,de la discriminación política en Venezuela. Caracas: Súmate.Producto Interno Bruto de Venezuela, 1957-2001. ” Revista

  7. Hctor L. Palacios Verdes Caracas, Venezuela

    E-Print Network [OSTI]

    Palacios, Héctor

    Héctor L. Palacios Verdes Caracas, Venezuela Phone: ask Email: hectorpal@gmail.com Web page: http://www.ldc.usb.ve/~hlp Citizen: Venezuela Residence: Venezuela Education Ph. D. Informatics and Digital Communication December. Caracas, Venezuela. Thesis: Planning as Branch and Bound: a Constraint Programming implementation. Advisor

  8. State and Power after Neoliberalism in Bolivarian Venezuela

    E-Print Network [OSTI]

    Kingsbury, Donald V.

    2012-01-01

    Gobierno Bolivariano de Venezuela. ———. 2005. Understandingp. 3–15. ———. 2007b. Venezuela: Hugo Chávez and the DeclineA Closed Bridge Mirrors Venezuela’s Many Woes. ” The New

  9. Hypochlorous acid and hydrogen peroxide-induced negative regulation of Salmonella enterica serovar Typhimurium ompW by the response regulator ArcA

    E-Print Network [OSTI]

    2012-01-01

    induced negative regulation of Salmonella enterica serovarsuperoxide dismutase in Salmonella. FEMS Microbiol LettAdaptation Sensitizes Salmonella Typhimurium to Hypochlorous

  10. Universidad Central de Venezuela Facultad de Ciencias

    E-Print Network [OSTI]

    Coto, Ernesto

    Universidad Central de Venezuela Facultad de Ciencias Escuela de Computación Lecturas en Ciencias ecoto@strix.ciens.ucv.ve ecoto@opalo.ciens.ucv.ve Universidad Central de Venezuela. Facultad de Ciencias. Escuela de Computación. Laboratorio de Computación Gráfica (LCG) Venezuela. Caracas Apdo. 47002, 1041-A

  11. Universidad Central de Venezuela Facultad de Ciencias

    E-Print Network [OSTI]

    Coto, Ernesto

    Universidad Central de Venezuela Facultad de Ciencias Escuela de Computación Lecturas en Ciencias ecoto@strix.ciens.ucv.ve ecoto@opalo.ciens.ucv.ve Universidad Central de Venezuela. Facultad de Ciencias. Escuela de Computación. Laboratorio de Computación Gráfica (LCG) Venezuela. Caracas Apdo. 47002, 1041-A ND

  12. Universidad Central de Venezuela Facultad de Ciencias

    E-Print Network [OSTI]

    Coto, Ernesto

    Universidad Central de Venezuela Facultad de Ciencias Escuela de Computación Una herramienta para. Universidad Central de Venezuela. {ecoto, hnavarro, omaira}@opalo.ciens.ucv.ve **Laboratorio TOOLS. Escuela de Computación. Facultad de Ciencias. Universidad Central de Venezuela. amatteo@isys.ciens.ucv.ve Caracas, Enero

  13. The Importance of Regulation-Induced Innovation for Sustainable Development

    E-Print Network [OSTI]

    Ashford, Nicholas A.

    This article explores the complex relationship between environmental regulation, innovation, and sustainable development within the context of an increasingly globalizing economy. The economic development, environment, and ...

  14. Engineered mRNA regulation using an inducible protein-RNA aptamer interaction

    E-Print Network [OSTI]

    Belmont, Brian J. (Brian Joshua)

    2012-01-01

    The importance and pervasiveness of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects ...

  15. Engineering a Direct and Inducible Protein-RNA Interaction To Regulate RNA Biology

    E-Print Network [OSTI]

    Belmont, Brian Joshua

    The importance and pervasiveness of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common mechanism uses inducible protein?RNA interactions to shape diverse aspects of cellular ...

  16. Retino-hypothalamic regulation of light-induced murine sleep

    E-Print Network [OSTI]

    Muindi, Fanuel

    The temporal organization of sleep is regulated by an interaction between the circadian clock and homeostatic processes. Light indirectly modulates sleep through its ability to phase shift and entrain the circadian clock. ...

  17. MAGI-3 regulates LPA-induced activation of Erk and RhoA Huanchun Zhang a

    E-Print Network [OSTI]

    Hall, Randy A

    MAGI-3 regulates LPA-induced activation of Erk and RhoA Huanchun Zhang a , Dongsheng Wang a , Hong of Erk and Akt. In contrast, silencing of MAGI-3 expression by siRNA drastically inhibited LPA-induced Erk activation, suggesting that the lack of an effect by overexpression was due to the high endogenous

  18. Plenia Locatel Group: Globalizing from Venezuela

    E-Print Network [OSTI]

    Gibson, Cyrus

    2009-05-01

    In 2009 the founders and top executives of Plenia Locatel Group, a retail business in Venezuela specializing in health care products and services, were planning a global

  19. MicroRNA Regulation of Ionizing Radiation-Induced Premature Senescence

    SciTech Connect (OSTI)

    Wang Yong; Scheiber, Melissa N.; Neumann, Carola; Calin, George A.; Zhou Daohong

    2011-11-01

    Purpose: MicroRNAs (miRNAs) have emerged as critical regulators of many cellular pathways. Ionizing radiation (IR) exposure causes DNA damage and induces premature senescence. However, the role of miRNAs in IR-induced senescence has not been well defined. Thus, the purpose of this study was to identify and characterize senescence-associated miRNAs (SA-miRNAs) and to investigate the role of SA-miRNAs in IR-induced senescence. Methods and Materials: In human lung (WI-38) fibroblasts, premature senescence was induced either by IR or busulfan (BU) treatment, and replicative senescence was accomplished by serial passaging. MiRNA microarray were used to identify SA-miRNAs, and real-time reverse transcription (RT)-PCR validated the expression profiles of SA-miRNAs in various senescent cells. The role of SA-miRNAs in IR-induced senescence was characterized by knockdown of miRNA expression, using anti-miRNA oligonucleotides or by miRNA overexpression through the transfection of pre-miRNA mimics. Results: We identified eight SA-miRNAs, four of which were up-regulated (miR-152, -410, -431, and -493) and four which were down-regulated (miR-155, -20a, -25, and -15a), that are differentially expressed in both prematurely senescent (induced by IR or BU) and replicatively senescent WI-38 cells. Validation of the expression of these SA-miRNAs indicated that down-regulation of miR-155, -20a, -25, and -15a is a characteristic miRNA expression signature of cellular senescence. Functional analyses revealed that knockdown of miR-155 or miR-20a, but not miR-25 or miR-15a, markedly enhanced IR-induced senescence, whereas ectopic overexpression of miR-155 or miR-20a significantly inhibited senescence induction. Furthermore, our studies indicate that miR-155 modulates IR-induced senescence by acting downstream of the p53 and p38 mitogen-activated protein kinase (MAPK) pathways and in part via regulating tumor protein 53-induced nuclear protein 1 (TP53INP1) expression. Conclusion: Our results suggest that SA-miRNAs are involved in the regulation of IR-induced senescence, so targeting these miRNAs may be a novel approach for modulating cellular response to radiation exposure.

  20. La República y las Letras — literatura y carácter nacional en Brasil y Venezuela

    E-Print Network [OSTI]

    Oliveira, Emanuelle K. F.

    1995-01-01

    nacional en Brasil y Venezuela* Introducción La Segundaes la nación (Brasil y Venezuela), pero este adentro sePor lo en esta nueva Venezuela es fundamental. Ésta será

  1. The Ambiguous Transition: Building State Capacity and Expanding Popular Participation in Venezuela's Agrarian Reform

    E-Print Network [OSTI]

    Page, Tiffany Linton

    2011-01-01

    El Universal, Caracas, Venezuela. Bengelsdorf, Carollee.Crisis and Reform in Venezuela. ” Journal of Interamerican2):27-61. Datamonitor. (2004). “Venezuela Country Profile. ”

  2. Reflections on relationships between national and world events and development of dermatology. Venezuela as a model

    E-Print Network [OSTI]

    Goihman-Yahr, Mauricio

    2013-01-01

    development of dermatology. Venezuela as a model MauricioVargas School of Medicine, Central University of Venezuela,Caracas Venezuela. ABSTRACT From a personal vantage, the

  3. Equal rights for equal action : women's mobilization for suffrage in Venezuela

    E-Print Network [OSTI]

    Skog, Erica Lynn

    2008-01-01

    and Dictatorship in Venezuela, 1945-1959. Hamden, CT: Archonand Political Change in Venezuela. Princeton, NJ: Princetonand the Church in Venezuela. ” Journal of Interamerican

  4. Hacia una redefinición del desarraigo: diálogos narrativos entre Mozambique y Venezuela

    E-Print Network [OSTI]

    López, Magdalena

    2014-01-01

    Banco Central de Venezuela. “Indicadores”. Internet. 20entre Mozambique y Venezuela MAGDALENA LÓPEZ UNIVERSIDADE DEen pedazos” (86). Venezuela, como la aldea de Tizangara,

  5. A soil bacterium regulates plant acquisition of iron via deficiency-inducible mechanisms

    E-Print Network [OSTI]

    Paré, Paul W.

    A soil bacterium regulates plant acquisition of iron via deficiency-inducible mechanisms Huiming in most soils. While certain soil microbes produce chelating agents that enhance the solubility of iron understanding that select soil microbes play a signaling role in activating growth and stress responses

  6. Autophagy induced by Alexander disease-mutant GFAP accumulation is regulated by p38/MAPK

    E-Print Network [OSTI]

    Sulzer, David

    and 2 Department of Neurology, Center for Parkinson's Disease and Other Movement Disorders, Columbia Disease, polyglutamine disease (CAG repeat), and Parkinson disease (10). Ã To whom correspondence shouldAutophagy induced by Alexander disease-mutant GFAP accumulation is regulated by p38/MAPK and m

  7. Reformulated gasoline deal with Venezuela draws heat

    SciTech Connect (OSTI)

    Begley, R.

    1994-04-06

    A fight is brewing in Congress over a deal to let Venezuela off the hook in complying with the Clean Air Act reformulated gasoline rule. When Venezuela threatened to call for a GATT panel to challenge the rule as a trade barrier, the Clinton Administration negotiated to alter the rule, a deal that members of Congress are characterizing as {open_quotes}secret{close_quotes} and {open_quotes}back door.{close_quotes}

  8. Festival Report: Experimental Theatre in Venezuela

    E-Print Network [OSTI]

    1985-10-01

    4 LATIN AMERICAN THEATRE REVIEW Experimental Theatre in Venezuela The I Jornadas de Búsqueda y Experimentación Teatral took place March 21-May 19, 1985 in Caracas. Organized by the Centro Latinoamericano de Creación e Investigación Teatral... of the festival was to give an opportunity for experimental theatre groups to present their work and thus to show the current tendencies of unconventional theatre in Venezuela. The participating groups (among them Shock Teatral, Contradanza, Taller...

  9. Massive Variability Surveys from Venezuela

    E-Print Network [OSTI]

    Cesar Briceno

    2003-04-03

    At the Venezuela National Astronomical Observatory we are carrying out variability surveys spanning many hundreds of square degrees near the celestial equator, using an 8k x 8k CCD Mosaic Camera optimized for drift-scanning on a 1m Schmidt telescope. Among the initial efforts was a project to obtain the first moderately deep, homogeneous sample of young stars over an area of ~180sqr.deg. encompassing the entire Orion OB1 association, one of the nearest and most active regions of star formation. The results show that variability is a powerful technique to identify pre-main sequence populations, specially in sparse areas devoid of gas and dust. We are currently developing a massive database, equipped with web-based data mining tools, that will make our data and results available to the astronomical community.

  10. PI3K/AKT and ERK regulate retinoic acid-induced neuroblastoma cellular differentiation

    SciTech Connect (OSTI)

    Qiao, Jingbo [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States)] [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Paul, Pritha; Lee, Sora [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States) [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Qiao, Lan; Josifi, Erlena; Tiao, Joshua R. [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States)] [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Chung, Dai H., E-mail: dai.chung@vanderbilt.edu [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232 (United States)

    2012-08-03

    Highlights: Black-Right-Pointing-Pointer Retinoic acid (RA) induces neuroblastoma cells differentiation, which is accompanied by G0/G1 cell cycle arrest. Black-Right-Pointing-Pointer RA resulted in neuroblastoma cell survival and inhibition of DNA fragmentation; this is regulated by PI3K pathway. Black-Right-Pointing-Pointer RA activates PI3K and ERK1/2 pathway; PI3K pathway mediates RA-induced neuroblastoma cell differentiation. Black-Right-Pointing-Pointer Upregulation of p21 is necessary for RA-induced neuroblastoma cell differentiation. -- Abstract: Neuroblastoma, the most common extra-cranial solid tumor in infants and children, is characterized by a high rate of spontaneous remissions in infancy. Retinoic acid (RA) has been known to induce neuroblastoma differentiation; however, the molecular mechanisms and signaling pathways that are responsible for RA-mediated neuroblastoma cell differentiation remain unclear. Here, we sought to determine the cell signaling processes involved in RA-induced cellular differentiation. Upon RA administration, human neuroblastoma cell lines, SK-N-SH and BE(2)-C, demonstrated neurite extensions, which is an indicator of neuronal cell differentiation. Moreover, cell cycle arrest occurred in G1/G0 phase. The protein levels of cyclin-dependent kinase inhibitors, p21 and p27{sup Kip}, which inhibit cell proliferation by blocking cell cycle progression at G1/S phase, increased after RA treatment. Interestingly, RA promoted cell survival during the differentiation process, hence suggesting a potential mechanism for neuroblastoma resistance to RA therapy. Importantly, we found that the PI3K/AKT pathway is required for RA-induced neuroblastoma cell differentiation. Our results elucidated the molecular mechanism of RA-induced neuroblastoma cellular differentiation, which may be important for developing novel therapeutic strategy against poorly differentiated neuroblastoma.

  11. Four New Hypancistrus (Siluriformes: Loricariidae) from Amazonas, Venezuela

    E-Print Network [OSTI]

    Armbruster, Jonathan W.

    Four New Hypancistrus (Siluriformes: Loricariidae) from Amazonas, Venezuela JONATHAN W. ARMBRUSTER. lunaorum are described based on specimens from the upper Ri´o Orinoco of southern Venezuela. Hypancistrus la base de especi´menes provenientes de la cuenca alta del ri´o Orinoco en el sur de Venezuela

  12. Light-induced nuclear import of phytochrome-A:GFP fusion proteins is differentially regulated in transgenic tobacco

    E-Print Network [OSTI]

    Schäfer, Eberhard

    Light-induced nuclear import of phytochrome-A:GFP fusion proteins is differentially regulated of the fusion protein was observed. By contrast, light-dependent nuclear import of the same fusion protein have recently shown that nuclear import of rice phyA:GFP is regulated by VLFR in transgenic tobacco

  13. Involvement of Extracellular Signal-Regulated Kinase (ERK) in Pardaxin-Induced Dopamine Release from PC12 Cells

    E-Print Network [OSTI]

    Linial, Michal

    Involvement of Extracellular Signal-Regulated Kinase (ERK) in Pardaxin-Induced Dopamine Release kinase (ERK)/mitogen-activated protein kinase (MAPK) in pardaxin-induced dopamine (DA) release DA release. Time course exper- iments indicated that PX, at nontoxic concentrations, stimu- lated ERK

  14. PKC{eta} is a negative regulator of AKT inhibiting the IGF-I induced proliferation

    SciTech Connect (OSTI)

    Shahaf, Galit; Rotem-Dai, Noa; Koifman, Gabriela; Raveh-Amit, Hadas; Frost, Sigal A.; Livneh, Etta, E-mail: etta@bgu.ac.il

    2012-04-15

    The PI3K-AKT pathway is frequently activated in human cancers, including breast cancer, and its activation appears to be critical for tumor maintenance. Some malignant cells are dependent on activated AKT for their survival; tumors exhibiting elevated AKT activity show sensitivity to its inhibition, providing an Achilles heel for their treatment. Here we show that the PKC{eta} isoform is a negative regulator of the AKT signaling pathway. The IGF-I induced phosphorylation on Ser473 of AKT was inhibited by the PKC{eta}-induced expression in MCF-7 breast adenocarcinoma cancer cells. This was further confirmed in shRNA PKC{eta}-knocked-down MCF-7 cells, demonstrating elevated phosphorylation on AKT Ser473. While PKC{eta} exhibited negative regulation on AKT phosphorylation it did not alter the IGF-I induced ERK phosphorylation. However, it enhanced ERK phosphorylation when stimulated by PDGF. Moreover, its effects on IGF-I/AKT and PDGF/ERK pathways were in correlation with cell proliferation. We further show that both PKC{eta} and IGF-I confer protection against UV-induced apoptosis and cell death having additive effects. Although the protective effect of IGF-I involved activation of AKT, it was not affected by PKC{eta} expression, suggesting that PKC{eta} acts through a different route to increase cell survival. Hence, our studies show that PKC{eta} provides negative control on AKT pathway leading to reduced cell proliferation, and further suggest that its presence/absence in breast cancer cells will affect cell death, which could be of therapeutic value.

  15. Venezuela natural gas for vehicles project

    SciTech Connect (OSTI)

    Marsicobetre, D.; Molero, T. [Corpoven S.A., Miami, FL (United States)

    1998-12-31

    The Natural Gas for Vehicles (NGV) Project in Venezuela describes the development and growth of the NGV project in the country. Venezuela is a prolific oil producer with advanced exploration, production, refining and solid marketing infrastructure. Gas production is 5.2 Bscfd. The Venezuelan Government and the oil state owned company Petroleos de Venezuela (PDVSA), pursued the opportunity of using natural gas for vehicles based on the huge amounts of gas reserves present and produced every day associated with the oil production. A nationwide gas pipeline network crosses the country from south to west reaching the most important cities and serving domestic and industrial purposes but there are no facilities to process or export liquefied natural gas. NGV has been introduced gradually in Venezuela over the last eight years by PDVSA. One hundred forty-five NGV stations have been installed and another 25 are under construction. Work done comprises displacement or relocation of existing gasoline equipment, civil work, installation and commissioning of equipment. The acceptance and usage of the NGV system is reflected in the more than 17,000 vehicles that have been converted to date using the equivalent of 2,000 bbl oil/day.

  16. Benzodiazepine Withdrawal-Induced Glutamatergic Plasticity Involves Up-Regulation of GluR1-Containing -Amino-3-

    E-Print Network [OSTI]

    Abraham, Nader G.

    Benzodiazepine Withdrawal-Induced Glutamatergic Plasticity Involves Up-Regulation of GluR1 and addiction. Benzodiazepine withdrawal results in increased glutamatergic strength, but whether -amino-3 remodeled during benzodiazepine withdrawal is uncertain. Whole-cell recordings of rat hippocampal CA1

  17. Human Glucocorticoid-Induced TNF Receptor Ligand Regulates its Signaling Activity Through Multiple Oligomerization States

    SciTech Connect (OSTI)

    Zhou,Z.; Dong, X.; Berezov, A.; Zhang, G.; Li, Y.; Zhang, H.; Murali, R.; Li, B.; Greene, M.

    2008-01-01

    Ligation between glucocorticoid-induced tumor necrosis factor receptor (GITR) and its ligand (GITRL) provides an undefined signal that renders CD4+CD25- effector T cells resistant to the inhibitory effects of CD4+CD25+ regulatory T cells. To understand the structural basis of GITRL function, we have expressed and purified the extracellular domain of human GITR ligand in Escherichia coli. Chromotography and cross-linking studies indicate that human GITRL (hGITRL) exists as dimers and trimers in solution and also can form a supercluster. To gain insight into the nature of GITRL oligomerization, we determined the crystallographic structures of hGITRL, which revealed a loosely associated open trimer with a deep cavity at the molecular center and a flexible C-terminal tail bent for trimerization. Moreover, a tetramer of trimers (i.e., supercluster) has also been observed in the crystal, consistent with the cross-linking analysis. Deletion of the C-terminal distal three residues disrupts the loosely assembled trimer and favors the formation of a dimer that has compromised receptor binding and signaling activity. Collectively, our studies identify multiple oligomeric species of hGITRL that possess distinct kinetics of ERK activation. The studies address the functional implications and structural models for a process by which hGITRL utilizes multiple oligomerization states to regulate GITR-mediated signaling during T cell costimulation.

  18. Martinez, Carlos, Michael Fox and JoJo Farrell. Venezuela Speaks! Voices from the Grassroots. Oakland, CA: PM Press, 2010. 343 pp.

    E-Print Network [OSTI]

    Farrell, Michelle Leigh

    2010-01-01

    well read specialists on Venezuela. This book represents anthe grassroots movements in Venezuela today. Michelle LeighFOX and JOJO FARRELL. Venezuela Speaks! Voices from the

  19. Late Quaternary climate change from delta 13 O records of multiple species of planktonic foraminifera: High-resolution records from the Anoxic Cariaco Basin, Venezuela

    E-Print Network [OSTI]

    Lin, Hui-Ling; Peterson, Larry C; Overpeck, Jonathan T; Trumbore, Susan E; Murray, David W

    1997-01-01

    the Lake Valencia Basin, Venezuela, Ecology, 66, 1279-1295,1954. waters of eastern Venezuela, Bol. Inst. Oceanogr.Los foraminiferos de Venezuela (resumen), Acta Geol. Hisp. ,

  20. e-Science perspectives in Venezuela

    E-Print Network [OSTI]

    Díaz, G; Hamar, V; Hoeger, H; Mendoza, C; Mendez, Z; Núñez, L A; Ruiz, N; Torrens, R; Uzcategui, M

    2008-01-01

    We describe the e-Science strategy in Venezuela, in particular initiatives by the Centro Nacional de Calculo Cientifico Universidad de Los Andes (CECALCULA), Merida, the Universidad de Los Andes (ULA), Merida, and the Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas. We present the plans for the Venezuelan Academic Grid and the current status of Grid ULA supported by Internet2. We show different web-based scientific applications that are being developed in quantum chemistry, atomic physics, structural damage analysis, biomedicine and bioclimate within the framework of the E-Infrastructure shared between Europe and Latin America (EELA)

  1. The Difference Between Certainly and Perhaps: The Bargaining Strategies of Venezuela and Argentina In Commercial Debt Negotiations

    E-Print Network [OSTI]

    Doherty, E.

    1995-01-01

    n.a. n.a. Brazil Mexico Venezuela Chile Total Note: BankThe Renegotiation of Venezuela's Foreign Debt During 1982of repayment was whether Venezuela would have to accept an

  2. A reservoir management strategy for multilayered reservoirs in eastern Venezuela 

    E-Print Network [OSTI]

    Espinel Diaz, Arnaldo Leopoldo

    1998-01-01

    A reservoir management strategy has been developed for a field located in eastern Venezuela. The field contains deep, high pressure, multilayer reservoirs. A thorough formation evaluation was accomplished using the log data, core data, PVT data...

  3. Tax effects upon oil field development in Venezuela

    E-Print Network [OSTI]

    Manzano, Osmel

    2000-01-01

    Important reforms have been made to the oil sector tax code in Venezuela. Given its diversity of oil resources, there was a concern that some resources were not being exploited because of the structure of the tax code. ...

  4. e-Science initiatives in Venezuela

    E-Print Network [OSTI]

    Chaves, J L; Hamar, V; Isea, R; Rojas, F; Ruiz, N; Torrens, R; Uzcategui, M; Florez-Lopez, J; Hoeger, H; Mendoza, C; Núñez, L A

    2007-01-01

    Within the context of the nascent e-Science infrastructure in Venezuela, we describe several web-based scientific applications developed at the Centro Nacional de Calculo Cientifico Universidad de Los Andes (CeCalCULA), Merida, and at the Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas. The different strategies that have been followed for implementing quantum chemistry and atomic physics applications are presented. We also briefly discuss a damage portal based on dynamic, nonlinear, finite elements of lumped damage mechanics and a biomedical portal developed within the framework of the \\textit{E-Infrastructure shared between Europe and Latin America} (EELA) initiative for searching common sequences and inferring their functions in parasitic diseases such as leishmaniasis, chagas and malaria.

  5. Ecology of large piscivorous fishes in Guri Reservoir, Venezuela, with notes on fish community structure 

    E-Print Network [OSTI]

    Williams, John David

    1995-01-01

    Venezuela's growing human population is accompanied by a growing need for electricity which has largely been met with hydroelectric power, and yet the full effects of river impoundment on river ecosystems are not known. Venezuela currently has...

  6. REGULATION OF CARBON METABOLISM IN BACTERIA 541 Smith, C.P. & Chater, K.F. (1988a), Cloning and tran-

    E-Print Network [OSTI]

    Redfield, Rosemary J. "Rosie"

    of a Streptomyces a-amylase gene. Micro- biology, 140, 1059-1067. Virolle, M.J., Long, C.M.. Chang, S., Bibb, M.J. (199"~. Cloning, characterisation and regulation of an ,,x- amylase gene from Streptomyces venezuela

  7. Residential energy use and conservation in Venezuela: Results and implications of a household survey in Caracas

    SciTech Connect (OSTI)

    Figueroa, M.J.; Ketoff, A.; Masera, O.

    1992-10-01

    This document presents the final report of a study of residential energy use in Caracas, the capital of Venezuela. It contains the findings of a household energy-use survey held in Caracas in 1988 and examines options for introducing energy conservation measures in the Venezuelan residential sector. Oil exports form the backbone of the Venezuelan economy. Improving energy efficiency in Venezuela will help free domestic oil resources that can be sold to the rest of the world. Energy conservation will also contribute to a faster recovery of the economy by reducing the need for major investments in new energy facilities, allowing the Venezuelan government to direct its financial investments towards other areas of development. Local environmental benefits will constitute an important additional by-product of implementing energy-efficiency policies in Venezuela. Caracas`s residential sector shows great potential for energy conservation. The sector is characterized by high saturation levels of major appliances, inefficiency of appliances available in the market, and by careless patterns of energy use. Household energy use per capita average 6.5 GJ/per year which is higher than most cities in developing countries; most of this energy is used for cooking. Electricity accounts for 41% of all energy use, while LPG and natural gas constitute the remainder. Specific options for inducing energy conservation and energy efficiency in Caracas`s residential sector include energy-pricing policies, fuel switching, particularly from electricity to gas, improving the energy performance of new appliances and customer information. To ensure the accomplishment of an energy-efficiency strategy, a concerted effort by energy users, manufacturers, utility companies, government agencies, and research institutions will be needed.

  8. Nitrogen isotope dynamics of the Cariaco Basin, Venezuela Robert C. Thunell,1

    E-Print Network [OSTI]

    Sigman, Daniel M.

    Nitrogen isotope dynamics of the Cariaco Basin, Venezuela Robert C. Thunell,1 Daniel M. Sigman,2 of Venezuela. Water column denitrification occurring in the basin has only a very small isotopic imprint-Karger, Y. Astor, and R. Varela (2004), Nitrogen isotope dynamics of the Cariaco Basin, Venezuela, Global

  9. POTENTIAL EFFECTS OF GLOBAL CLIMATIC CHANGE ON THE PHENOLOGY AND YIELD OF MAIZE IN VENEZUELA *

    E-Print Network [OSTI]

    Robock, Alan

    POTENTIAL EFFECTS OF GLOBAL CLIMATIC CHANGE ON THE PHENOLOGY AND YIELD OF MAIZE IN VENEZUELA de Ciencias, Universidad de Los Andes, Mdrida 5101, Venezuela 21nstitute of Applied Sciences, Venezuela 4Centro de Estudios Avanzados del Clima Tropical (CEACT), Ministerio deI Ambiente y de los

  10. BREEDING BIOLOGY OF THE GOLDEN-FACED TYRANNULET (ZIMMERIUS CHRYSOPS) IN VENEZUELA

    E-Print Network [OSTI]

    Martin, Thomas E.

    BREEDING BIOLOGY OF THE GOLDEN-FACED TYRANNULET (ZIMMERIUS CHRYSOPS) IN VENEZUELA WILLIAM GOULDING1 Park, Venezuela during the 2002 to 2008 breeding seasons. The enclosed nest was similar to descriptions, it is broadly distributed with a range that extends from Peru, north through the Andes to Colombia and Venezuela

  11. Receiver function study of the crustal structure of the southeastern Caribbean plate boundary and Venezuela

    E-Print Network [OSTI]

    Niu, Fenglin

    and Venezuela Fenglin Niu,1 Tammy Bravo,2 Gary Pavlis,2 Frank Vernon,3 Herbert Rendon,4 Maximiliano Bezada,1 broadband array deployed under the BOLIVAR project and the permanent national seismic network of Venezuela northeastern Venezuela and the Venezuelan Andes. There is a good correlation between crustal structure

  12. BREEDING BIOLOGY AND NATURAL HISTORY OF THE SLATE-THROATED WHITESTART IN VENEZUELA

    E-Print Network [OSTI]

    Martin, Thomas E.

    BREEDING BIOLOGY AND NATURAL HISTORY OF THE SLATE-THROATED WHITESTART IN VENEZUELA ROMA´ N A´ National Park, Venezuela. Nesting activity peaked in late April and May. Only the female built the nest is the most common race in the Andes of Venezuela, Colombia, and part of Ecuador. Genetic information for our

  13. TWO NEW SPECIES OF LEPIDOCYRTUS FROM PARAMO DE MUCUBAJI MERIDA, VENEZUELA

    E-Print Network [OSTI]

    Mari-Mutt, José A.

    TWO NEW SPECIES OF LEPIDOCYRTUS FROM PARAMO DE MUCUBAJI MERIDA, VENEZUELA (COLLEMBOLA ejemplares colectados en Paramo de Mucubaji, Estado de Merida, Venezuela. Los individuos fueron encontrados in Venezuela but only one, L. summersii (MacGillivray) 1894, has been described or reported from this country

  14. NATURAL HAZARDS ON ALLUVIAL FANS: THE VENEZUELA DEBRIS FLOW AND FLASH FLOOD DISASTER

    E-Print Network [OSTI]

    Eaton, L. Scott

    NATURAL HAZARDS ON ALLUVIAL FANS: THE VENEZUELA DEBRIS FLOW AND FLASH FLOOD DISASTER In December Venezuela. Rainfall on December 2-3 totaled 200 millimeters (8 inches) and was followed by a major storm, Denver, Colorado, Naples, Italy, and Vargas, Venezuela. In time scales spanning thousands of years

  15. Mantle flow beneath northwestern Venezuela: Seismic evidence for a deep origin of the Mrida Andes

    E-Print Network [OSTI]

    Niu, Fenglin

    Mantle flow beneath northwestern Venezuela: Seismic evidence for a deep origin of the Mérida Andes University, Houston, TX, USA b Fundación de Investigaciones Simológicas (FUNVISIS), Caracas, Venezuela a b wave splitting from SKS data recorded by the national seismic network of Venezuela and a linear

  16. Political Bots and the Manipulation of Public Opinion in Venezuela

    E-Print Network [OSTI]

    Forelle, Michelle; Monroy-Hernández, Andrés; Savage, Saiph

    2015-01-01

    Social and political bots have a small but strategic role in Venezuelan political conversations. These automated scripts generate content through social media platforms and then interact with people. In this preliminary study on the use of political bots in Venezuela, we analyze the tweeting, following and retweeting patterns for the accounts of prominent Venezuelan politicians and prominent Venezuelan bots. We find that bots generate a very small proportion of all the traffic about political life in Venezuela. Bots are used to retweet content from Venezuelan politicians but the effect is subtle in that less than 10 percent of all retweets come from bot-related platforms. Nonetheless, we find that the most active bots are those used by Venezuela's radical opposition. Bots are pretending to be political leaders, government agencies and political parties more than citizens. Finally, bots are promoting innocuous political events more than attacking opponents or spreading misinformation.

  17. Inhibition of the ERK phosphorylation plays a role in terbinafine-induced p21 up-regulation and DNA synthesis inhibition in human vascular endothelial cells

    SciTech Connect (OSTI)

    Ho, P.-Y. [Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei, Taiwan (China); Hsu, S.-P. [Institute of Physiology, College of Medicine, National Taiwan University, Taiwan (China); Liang, Y.-C. [Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan (China); Kuo, M.-L. [Institute of Toxicology, College of Medicine, National Taiwan University, Taiwan (China); Ho, Y.-S. [Graduate Institute of Biomedical Technology, Taipei Medical University, Taipei, Taiwan (China); Lee, W.-S. [Graduate Institute of Neuroscience, Taipei Medical University, Taipei, Taiwan (China); Graduate Institute of Medical Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan (China); Department of Physiology, Medical College, Taipei Medical University, Taipei, Taiwan (China)], E-mail: wslee@tmu.edu.tw

    2008-05-15

    Previously, we showed that terbinafine (TB) induces cell-cycle arrest in cultured human umbilical vein endothelial cells (HUVEC) through an up-regulation of the p21 protein. The aim of this study is to delineate the molecular mechanisms underlying TB-induced increase of p21 protein. RT-PCR analysis demonstrated that the mRNA levels of p21 and p53 were increased in the TB-treated HUVEC. The p21 promoter activity was also increased by TB treatment. Transfection of HUVEC with p53 dominant negative (DN) abolished the TB-induced increases of p21 promoter activity and protein level, suggesting that the TB-induced increase of p21 is p53-dependent. Western blot analysis demonstrated that TB decreased the levels of phosphorylated extracellular signal-regulated kinase (ERK). Over-expression of mitogen-activated protein kinase (MEK)-1, the immediate upstream activator kinase of ERK, abolished the TB-induced increases of p21 and p53 protein and decrease of thymidine incorporation. The ERK inhibitor (PD98059) enhanced the TB-induced inhibition of thymidine incorporation into HUVEC. Taken together, these data suggest that the decrease of ERK activity plays a role in the TB-induced up-regulation of p21 in HUVEC. On the other hand, pretreatment of the cells with geranylgeraniol (GGOH), farnesol (FOH), or Ras inhibitor peptide did not affect the TB-induced decrease of thymidine incorporation. Taken together, our results suggest that TB might cause a decrease of MEK, which in turn up-regulates p53 through the inhibition of ERK phosphorylation, and finally causes an increase of p21 expression and cell-cycle arrest.

  18. TRPM7 channel regulates PDGF-BB-induced proliferation of hepatic stellate cells via PI3K and ERK pathways

    SciTech Connect (OSTI)

    Fang, Ling, E-mail: fangling_1984@126.com; Zhan, Shuxiang; Huang, Cheng; Cheng, Xi; Lv, Xiongwen; Si, Hongfang; Li, Jun, E-mail: lj@ahmu.edu.cn

    2013-11-01

    TRPM7, a non-selective cation channel of the TRP channel superfamily, is implicated in diverse physiological and pathological processes including cell proliferation. Recently, TRPM7 has been reported in hepatic stellate cells (HSCs). Here, we investigated the contribution role of TRPM7 in activated HSC-T6 cell (a rat hepatic stellate cell line) proliferation. TRPM7 mRNA and protein were measured by RT-PCR and Western blot in rat model of liver fibrosis in vivo and PDGF-BB-activated HSC-T6 cells in vitro. Both mRNA and protein of TRPM7 were dramatically increased in CCl{sub 4}-treated rat livers. Stimulation of HSC-T6 cells with PDGF-BB resulted in a time-dependent increase of TRPM7 mRNA and protein. However, PDGF-BB-induced HSC-T6 cell proliferation was inhibited by non-specific TRPM7 blocker 2-aminoethoxydiphenyl borate (2-APB) or synthetic siRNA targeting TRPM7, and this was accompanied by downregulation of cell cycle proteins, cyclin D1, PCNA and CDK4. Blockade of TRPM7 channels also attenuated PDGF-BB induced expression of myofibroblast markers as measured by the induction of ?-SMA and Col1?1. Furthermore, the phosphorylation of ERK and AKT, associated with cell proliferation, decreased in TRPM7 deficient HSC-T6 cells. These observations suggested that TRPM7 channels contribute to perpetuated fibroblast activation and proliferation of PDGF-BB induced HSC-T6 cells via the activation of ERK and PI3K pathways. Therefore, TRPM7 may constitute a useful target for the treatment of liver fibrosis. - Highlights: • Upregulation of TRPM7 mRNA and protein in the fibrotic livers from CCl{sub 4}-treated rats. • Increasing expression of TRPM7 mRNA and protein during HSC activation. • Blockade of TRPM7 inhibited the PDGF-BB induced proliferation of HSC-T6 cells. • Blockade of TRPM7 decreased ?-SMA and Col1?1 expressions in activated HSC-T6 cells. • TRPM7 up-regulation contributes to the activation of ERK and AKT pathways.

  19. An investigation of rainfall variability and distribution in Venezuela 

    E-Print Network [OSTI]

    Gibson, Donald Reid

    1968-01-01

    '966. Grosske, F. , Sabre I a Pluviosidad en Caracas, Universidad Central De Venezuela, Facultad De Ingenieria, Depart- amento De Meteorogia e Hidrologia, 144 pp. , Marzo 1967. Henry, tI. K. , Gr if'ithes, 3- F . , Cobb, G. L. and Coligado, M. C. , "Research...

  20. Human p38{delta} MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14

    SciTech Connect (OSTI)

    Ozawa, Shigeyuki [Oral Health Science Research Center (Japan) [Oral Health Science Research Center (Japan); Department of Biochemistry and Molecular Biology (Japan); Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka 238-8580 (Japan); Ito, Shin; Kato, Yasumasa [Oral Health Science Research Center (Japan) [Oral Health Science Research Center (Japan); Department of Biochemistry and Molecular Biology (Japan); Kubota, Eiro [Department of Biochemistry and Molecular Biology (Japan) [Department of Biochemistry and Molecular Biology (Japan); Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka 238-8580 (Japan); Hata, Ryu-Ichiro, E-mail: ryuhata@gmail.com [Oral Health Science Research Center (Japan) [Oral Health Science Research Center (Japan); Department of Biochemistry and Molecular Biology (Japan)

    2010-06-11

    The mitogen-activated protein kinase (MAPK) family comprises ERK, JNK, p38 and ERK5 (big-MAPK, BMK1). UV irradiation of squamous cell carcinoma cells induced up-regulation of gene expression of chemokine BRAK/CXCL14, stimulated p38 phosphorylation, and down-regulated the phosphorylation of ERK. Human p38 MAPKs exist in 4 isoforms: p38{alpha}, {beta}, {gamma} and {delta}. The UV stimulation of p38 phosphorylation was not inhibited by the presence of SB203580 or PD169316, inhibitors of p38{alpha} and {beta}, suggesting p38 phosphorylation was not dependent on these 2 isoforms and that p38{gamma} and/or {delta} was responsible for the phosphorylation. In fact, inhibition of each of these 4 p38 isoforms by the introduction of short hairpin (sh) RNAs for respective isoforms revealed that only shRNA for p38{delta} attenuated the UV-induced up-regulation of BRAK/CXCL14 gene expression. In addition, over-expression of p38 isoforms in the cells showed the association of p38{delta} with ERK1 and 2, concomitant with down-regulation of ERK phosphorylation. The usage of p38{delta} isoform by UV irradiation is not merely due to the abundance of this p38 isoform in the cells. Because serum deprivation of the cells also induced an increase in BRAK/CXCL14 gene expression, and in this case p38{alpha} and/or {beta} isoform is responsible for up-regulation of BRAK/CXCL14 gene expression. Taken together, the data indicate that the respective stress-dependent action of p38 isoforms is responsible for the up-regulation of the gene expression of the chemokine BRAK/CXCL14.

  1. Spleen tyrosine kinase mediates high glucose-induced transforming growth factor-{beta}1 up-regulation in proximal tubular epithelial cells

    SciTech Connect (OSTI)

    Yang, Won Seok; Chang, Jai Won [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); Han, Nam Jeong [Department of Cell Biology, Asan Institute for Life Sciences, Seoul (Korea, Republic of)] [Department of Cell Biology, Asan Institute for Life Sciences, Seoul (Korea, Republic of); Lee, Sang Koo [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); Park, Su-Kil, E-mail: skpark@amc.seoul.kr [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)

    2012-09-10

    The role of spleen tyrosine kinase (Syk) in high glucose-induced intracellular signal transduction has yet to be elucidated. We investigated whether Syk is implicated in high glucose-induced transforming growth factor-{beta}1 (TGF-{beta}1) up-regulation in cultured human proximal tubular epithelial cells (HK-2 cell). High glucose increased TGF-{beta}1 gene expression through Syk, extracellular signal-regulated kinase (ERK), AP-1 and NF-{kappa}B. High glucose-induced AP-1 DNA binding activity was decreased by Syk inhibitors and U0126 (an ERK inhibitor). Syk inhibitors suppressed high glucose-induced ERK activation, whereas U0126 had no effect on Syk activation. High glucose-induced NF-{kappa}B DNA binding activity was also decreased by Syk inhibitors. High glucose increased nuclear translocation of p65 without serine phosphorylation of I{kappa}B{alpha} and without degradation of I{kappa}B{alpha}, but with an increase in tyrosine phosphorylation of I{kappa}B{alpha} that may account for the activation of NF-{kappa}B. Both Syk inhibitors and Syk-siRNA attenuated high glucose-induced I{kappa}B{alpha} tyrosine phosphorylation and p65 nuclear translocation. Depletion of p21-activated kinase 2 (Pak2) by transfection of Pak2-siRNA abolished high glucose-induced Syk activation. In summary, high glucose-induced TGF-{beta}1 gene transcription occurred through Pak2, Syk and subsequent ERK/AP-1 and NF-{kappa}B pathways. This suggests that Syk might be implicated in the diabetic kidney disease.

  2. The Ambiguous Transition: Building State Capacity and Expanding Popular Participation in Venezuela's Agrarian Reform

    E-Print Network [OSTI]

    Page, Tiffany Linton

    2011-01-01

    the Democracy? Crisis and Reform in Venezuela. ” Journal ofLaura. 1997. Agrarian Reform and Class Consciousness inCristóbal. 1978. “Agrarian Reform and the Class Struggle in

  3. Clay mineralogy of surface sediments as a tool for deciphering river contributions to the Cariaco Basin (Venezuela)

    E-Print Network [OSTI]

    Meyers, Steven D.

    Basin (Venezuela) V. Bout-Roumazeilles,1 A. Riboulleau,1 E. Armynot du Châtelet,1 L. Lorenzoni,3 N for deciphering river contributions to the Cariaco Basin (Venezuela), J. Geophys. Res. Oceans, 118, doi:10

  4. Comparison of species-specific oxygen isotope paleotemperature equations: Sensitivity analysis using planktonic foraminifera from the Cariaco Basin, Venezuela

    E-Print Network [OSTI]

    Meyers, Steven D.

    using planktonic foraminifera from the Cariaco Basin, Venezuela Katherine E. Wejnert a, , Robert C, Venezuela a b s t r a c ta r t i c l e i n f o Article history: Received 6 December 2011 Received in revised biweekly sediment trap samples collected in Cariaco Basin, Venezuela between 2003 and 2008. Sixteen

  5. Development, Antibiotic Production, and Ribosome Assembly in Streptomyces venezuelae Are Impacted by RNase J and RNase III

    E-Print Network [OSTI]

    Elliot, Marie A.

    Development, Antibiotic Production, and Ribosome Assembly in Streptomyces venezuelae Are Impacted model system Streptomyces venezuelae. We show that each enzyme makes a unique contribution to the growth and development of S. venezuelae and further affects the secondary metabolism and antibiotic production

  6. Two new Fusicoccum species from Acacia and Eucalyptus in Venezuela, based on morphology and DNA sequence data

    E-Print Network [OSTI]

    Two new Fusicoccum species from Acacia and Eucalyptus in Venezuela, based on morphology and DNA in Venezuela, based on morphological features in culture and comparisons of DNA se- quence data. The two taxa y Ambientales, Universidad de los Andes, Me´rida 5101-A, Venezuela. available at www

  7. Pathogenicity of seven species of the Botryosphaeriaceae on Eucalyptus clones in Venezuela

    E-Print Network [OSTI]

    Pathogenicity of seven species of the Botryosphaeriaceae on Eucalyptus clones in Venezuela S. R. The Botryosphaeriaceae include several well recognised Eucalyptus pathogens of which various species have recently been found on Eucalyptus spp. in Venezuela. An initial inoculation trial was conducted using seven species

  8. JBP485 improves gentamicin-induced acute renal failure by regulating the expression and function of Oat1 and Oat3 in rats

    SciTech Connect (OSTI)

    Guo, Xinjin [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Meng, Qiang; Liu, Qi; Wang, Changyuan; Sun, Huijun; Peng, Jinyong; Ma, Xiaochi [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Dalian Medical University, Liaoning (China); Kaku, Taiichi [Japan Bioproducts Industry Co. Ltd., Tomigaya, Shibuya-ku, Tokyo (Japan); Liu, Kexin, E-mail: kexinliu@dlmedu.edu.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Dalian Medical University, Liaoning (China)

    2013-09-01

    We investigated the effects of JBP485 (an anti-inflammatory dipeptide and a substrate of OAT) on regulation of the expression and function of renal Oat1 and Oat3, which can accelerate the excretion of accumulated uremic toxins (e.g. indoxyl sulfate) in the kidney to improve gentamicin-induced ARF in rats. JBP485 caused a significant decrease in the accumulation of endogenous substances (creatinine, blood urea nitrogen and indoxyl sulfate) in vivo, an increase in the excretion of exogenous compounds (lisinopril and inulin) into urine, and up-regulation of the expressions of renal Oat1 and Oat3 in the kidney tissues and slices via substrate induction. To determine the effect of JBP485 on the accelerated excretion of uremic toxins mediated by Oat1 and Oat3, the mRNA and protein expression levels of renal basolateral Oats were assessed by quantitative real-time PCR, western blot, immunohistochemical analysis and an immunofluorescence method. Gentamicin down-regulated the expression of Oats mRNA and protein in rat kidney, and these effects were reversed after administration of JBP485. In addition, JBP485 caused a significant decrease in MPO and MDA levels in the kidney, and improved the pathological condition of rat kidney. These results indicated that JBP485 improved acute renal failure by increasing the expression and function of Oat1 and Oat3, and by decreasing overoxidation of the kidney in gentamicin-induced ARF rats. - Highlights: • JBP485 could up-regulate function and expression of Oat1 and Oat3 in kidney. • Effects of JBP485 on ARF are mediated by stimulating excretion of uremic toxins. • JBP485 protected against gentamicin-induced ARF by decreasing MPO and MDA.

  9. Lactoferrin inhibits dexamethasone-induced chondrocyte impairment from osteoarthritic cartilage through up-regulation of extracellular signal-regulated kinase 1/2 and suppression of FASL, FAS, and Caspase 3

    SciTech Connect (OSTI)

    Tu, Yihui [Department of Orthopaedics, Yangpu District Central Hospital Affiliated to Tongji University School of Medicine, 450 Tengyue Road, Shanghai (China)] [Department of Orthopaedics, Yangpu District Central Hospital Affiliated to Tongji University School of Medicine, 450 Tengyue Road, Shanghai (China); Xue, Huaming [Department of Orthopaedics, Yangpu District Central Hospital Affiliated to Tongji University School of Medicine, 450 Tengyue Road, Shanghai (China) [Department of Orthopaedics, Yangpu District Central Hospital Affiliated to Tongji University School of Medicine, 450 Tengyue Road, Shanghai (China); Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom); Francis, Wendy [Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom)] [Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom); Davies, Andrew P. [Department of Orthopaedics and Trauma, Moriston Hospital, Swansea (United Kingdom)] [Department of Orthopaedics and Trauma, Moriston Hospital, Swansea (United Kingdom); Pallister, Ian; Kanamarlapudi, Venkateswarlu [Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom)] [Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom); Xia, Zhidao, E-mail: zhidao.xia@gmail.com [Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom)] [Institute of Life Science, College of Medicine, Swansea University, Singleton Park (United Kingdom)

    2013-11-08

    Highlights: •Dex exerts dose-dependant inhibition of HACs viability and induction of apoptosis. •Dex-induced impairment of chondrocytes was attenuated by rhLF. •ERK and FASL/FAS signaling are involved in the effects of rhLF. •OA patients with glucocorticoid-induced cartilage damage may benefit from treatment with rhLF. -- Abstract: Dexamethasone (Dex) is commonly used for osteoarthritis (OA) with excellent anti-inflammatory and analgesic effect. However, Dex also has many side effects following repeated use over prolonged periods mainly through increasing apoptosis and inhibiting proliferation. Lactoferrin (LF) exerts significantly anabolic effect on many cells and little is known about its effect on OA chondrocytes. Therefore, the aim of this study is to investigate whether LF can inhibit Dex-induced OA chondrocytes apoptosis and explore its possible molecular mechanism involved in. MTT assay was used to determine the optimal concentration of Dex and recombinant human LF (rhLF) on chondrocytes at different time and dose points. Chondrocytes were then stimulated with Dex in the absence or presence of optimal concentration of rhLF. Cell proliferation and viability were evaluated using MTT and LIVE/DEAD assay, respectively. Cell apoptosis was evaluated by multi-parameter apoptosis assay kit using both confocal microscopy and flow cytometry, respectively. The expression of extracellular signal-regulated kinase (ERK), FAS, FASL, and Caspase-3 (CASP3) at the mRNA and protein levels were examined by real-time polymerase chain reaction (PCR) and immunocytochemistry, respectively. The optimal concentration of Dex (25 ?g/ml) and rhLF (200 ?g/ml) were chosen for the following experiments. rhLF significantly reversed the detrimental effect of Dex on chondrocytes proliferation, viability, and apoptosis. In addition, rhLF significantly prevented Dex-induced down-regulation of ERK and up-regulation of FAS, FASL, and CASP3. These findings demonstrated that rhLF acts as an anabolic effect on chondrocytes through significantly reversing Dex-induced chondrocytes apoptosis. This study may contribute to further investigating the clinical application of LF on OA.

  10. Moderate extracellular acidification inhibits capsaicin-induced cell death through regulating calcium mobilization, NF-{kappa}B translocation and ROS production in synoviocytes

    SciTech Connect (OSTI)

    Hu, Fen; Yang, Shuang; Zhao, Dan; Zhu, Shuyan; Wang, Yuxiang; Li, Junying

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Moderate extracellular acidification regulates intracellular Ca{sup 2+} mobilization. Black-Right-Pointing-Pointer Moderate acidification activates NF-{kappa}B nuclear translocation in synoviocytes. Black-Right-Pointing-Pointer Moderate acidification depresses the ROS production induced by capsaicin. Black-Right-Pointing-Pointer Moderate acidification inhibits capsaicin-caused synoviocyte death. -- Abstract: We previously show the expression of transient receptor potential vanilloid 1 (TRPV1) in primary synoviocytes from collagen-induced arthritis (CIA) rats. Capsaicin and lowered extracellular pH from 7.4 to 5.5 induce cell death through TRPV1-mediated Ca{sup 2+} entry and reactive oxygen species (ROS) production. However, under the pathological condition in rheumatoid arthritis, the synovial fluid is acidified to a moderate level (about pH 6.8). In the present study, we examined the effects of pH 6.8 on the TRPV1-mediated cell death. Our finding is different or even opposite from what was observed at pH 5.5. We found that the moderate extracellular acidification (from pH 7.4 to 6.8) inhibited the capsaicin-induced Ca{sup 2+} entry through attenuating the activity of TRPV1. In the mean time, it triggered a phospholipse C (PLC)-related Ca{sup 2+} release from intracellular stores. The nuclear translocation of NF-{kappa}B was found at pH 6.8, and this also depends on PLC activation. Moreover, the capsaicin-evoked massive ROS production and cell death were depressed at pH 6.8, both of which are dependent on the activation of PLC and NF-{kappa}B. Taken together, these results suggested that the moderate extracellular acidification inhibited the capsaicin-induced synoviocyte death through regulating Ca{sup 2+} mobilization, activating NF-{kappa}B nuclear translocation and depressing ROS production.

  11. Identification of Significantly Regulated Genes in the Estrogen Induced Gallus gallus Liver Over a 24-Hour Time Course 

    E-Print Network [OSTI]

    Trojacek, Erica

    2012-02-14

    In birds, estrogen is a strong stimulator of gene programs that regulate the formation of very low density lipoproteins (VLDL). Apolipoprotein-B (ApoB) is an integral part of very low density lipoproteins. In mammals, ...

  12. Regulation of Obesity-Induced Adipose Tissue Inflammation and Insulin Resistance through Modulation of Immune Cell Function 

    E-Print Network [OSTI]

    Ying, Wei

    2015-02-02

    The recruitment of immune cells into adipose tissue is a hallmark of obesity and a key event contributing to the development of adipose tissue inflammation and insulin resistance. However, the mechanisms regulating functions ...

  13. Identification and characterization of Hydraulic Flow Units in the San Juan Formation, Orocual Field, Venezuela 

    E-Print Network [OSTI]

    Deghirmandjian, Odilia

    2001-01-01

    This thesis focuses on the integration of core and well log data in order to provide a petrophysical characterization of the Hydraulic Flow Units (HFU) in the San Juan Formation, Orocual Field, Venezuela. We used three separate approaches...

  14. A revision of the Larainae (Coleoptera, Elmidae) of Venezuela, with description of nine new species

    E-Print Network [OSTI]

    Maier, Crystal A.

    2013-09-05

    The species of the riffle beetle subfamily Larainae occurring in Venezuela are revised. Examination of 756 specimens yielded 22 species in nine genera occurring throughout the country. Seven species are newly recorded from the country: Phanoceroides...

  15. Ecology of the river dolphin, Inia geoffrensis, in the Cinaruco River, Venezuela 

    E-Print Network [OSTI]

    McGuire, Tamara Lee

    1995-01-01

    The Cinaruco River is a tributary of the Orinoco River, and forms the southern boundary of Venezuela's newest national park, Santos Luzardo. Like other rivers of this region, the Cinaruco River undergoes an extreme seasonal flood cycle. River...

  16. Characterization of gas condensate reservoirs using pressure transient and production data - Santa Barbara Field, Monagas, Venezuela 

    E-Print Network [OSTI]

    Medina Tarrazzi, Trina Mercedes

    2003-01-01

    This thesis presents a field case history of the integrated analysis and interpretation developed using all of the available petrophysical, production, and well test data from the condensate zone of Block A, Santa Barbara Field (Monagas, Venezuela...

  17. Different methodologies for obtaining a permeability distribution in the Misoa Formation, Venezuela 

    E-Print Network [OSTI]

    Garcia, Ivette

    1998-01-01

    correlation as a function of porosity and grain diameter based on systematic packing. These two methodologies have been used to determine a permeability profile in the Misoa Formation, Venezuela. Formation permeability controls the strategies involving well...

  18. Roles of Sensory Nerves in the Regulation of Radiation-Induced Structural and Functional Changes in the Heart

    SciTech Connect (OSTI)

    Sridharan, Vijayalakshmi; Tripathi, Preeti [Department of Pharmaceutical Sciences, Division of Radiation Health, University of Arkansas for Medical Sciences, Little Rock, Arkansas (United States); Sharma, Sunil [Department of Radiation Oncology, Division of Radiation Health, University of Arkansas for Medical Sciences, Little Rock, Arkansas (United States); Moros, Eduardo G. [Department of Radiation Oncology, Moffitt Cancer Center and Research Institute, Tampa, Florida (United States); Zheng, Junying [Department of Pharmaceutical Sciences, Division of Radiation Health, University of Arkansas for Medical Sciences, Little Rock, Arkansas (United States); Hauer-Jensen, Martin [Department of Pharmaceutical Sciences, Division of Radiation Health, University of Arkansas for Medical Sciences, Little Rock, Arkansas (United States); Surgical Service, Central Arkansas Veterans Healthcare System, Little Rock, Arkansas (United States); Boerma, Marjan, E-mail: mboerma@uams.edu [Department of Pharmaceutical Sciences, Division of Radiation Health, University of Arkansas for Medical Sciences, Little Rock, Arkansas (United States)

    2014-01-01

    Purpose: Radiation-induced heart disease (RIHD) is a chronic severe side effect of radiation therapy of intrathoracic and chest wall tumors. The heart contains a dense network of sensory neurons that not only are involved in monitoring of cardiac events such as ischemia and reperfusion but also play a role in cardiac tissue homeostasis, preconditioning, and repair. The purpose of this study was to examine the role of sensory nerves in RIHD. Methods and Materials: Male Sprague-Dawley rats were administered capsaicin to permanently ablate sensory nerves, 2 weeks before local image-guided heart x-ray irradiation with a single dose of 21 Gy. During the 6 months of follow-up, heart function was assessed with high-resolution echocardiography. At 6 months after irradiation, cardiac structural and molecular changes were examined with histology, immunohistochemistry, and Western blot analysis. Results: Capsaicin pretreatment blunted the effects of radiation on myocardial fibrosis and mast cell infiltration and activity. By contrast, capsaicin pretreatment caused a small but significant reduction in cardiac output 6 months after irradiation. Capsaicin did not alter the effects of radiation on cardiac macrophage number or indicators of autophagy and apoptosis. Conclusions: These results suggest that sensory nerves, although they play a predominantly protective role in radiation-induced cardiac function changes, may eventually enhance radiation-induced myocardial fibrosis and mast cell activity.

  19. Library Regulations Library Regulations

    E-Print Network [OSTI]

    Birmingham, University of

    Library Regulations 2012-13 Library Regulations UNIVERSITY OF BIRMINGHAM REGULATIONS LIBRARY REGULATIONS Preamble: The Library Regulations apply to all users of library facilities managed on behalf of the University by Library Services, and thus there are sections that apply also to non- members of the University

  20. Proteasome inhibition-induced p38 MAPK/ERK signaling regulates autophagy and apoptosis through the dual phosphorylation of glycogen synthase kinase 3{beta}

    SciTech Connect (OSTI)

    Choi, Cheol-Hee [Research Center for Resistant Cells, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759 (Korea, Republic of) [Research Center for Resistant Cells, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759 (Korea, Republic of); Department of Pharmacology, College of Medicine, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759 (Korea, Republic of); Lee, Byung-Hoon [College of Pharmacy and Multiscreening Center for Drug Development, Seoul National University, Seoul 151-742 (Korea, Republic of)] [College of Pharmacy and Multiscreening Center for Drug Development, Seoul National University, Seoul 151-742 (Korea, Republic of); Ahn, Sang-Gun [Department of Pathology, College of Dentistry, Chosun University, Gwangju 501-759 (Korea, Republic of)] [Department of Pathology, College of Dentistry, Chosun University, Gwangju 501-759 (Korea, Republic of); Oh, Seon-Hee, E-mail: oshccw@hanmail.net [Research Center for Resistant Cells, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759 (Korea, Republic of)] [Research Center for Resistant Cells, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759 (Korea, Republic of)

    2012-02-24

    Highlights: Black-Right-Pointing-Pointer MG132 induces the phosphorylation of GSK3{beta}{sup Ser9} and, to a lesser extent, of GSK3{beta}{sup Thr390}. Black-Right-Pointing-Pointer MG132 induces dephosphorylation of p70S6K{sup Thr389} and phosphorylation of p70S6K{sup Thr421/Ser424}. Black-Right-Pointing-Pointer Inactivation of p38 dephosphorylates GSK3{beta}{sup Ser9} and phosphorylates GSK3{beta}{sup Thr390}. Black-Right-Pointing-Pointer Inactivation of p38 phosphorylates p70S6K{sup Thr389} and increases the phosphorylation of p70S6K{sup Thr421/Ser424}. Black-Right-Pointing-Pointer Inactivation of p38 decreases autophagy and increases apoptosis induced by MG132. -- Abstract: Proteasome inhibition is a promising approach for cancer treatment; however, the underlying mechanisms involved have not been fully elucidated. Here, we show that proteasome inhibition-induced p38 mitogen-activated protein kinase regulates autophagy and apoptosis by modulating the phosphorylation status of glycogen synthase kinase 3{beta} (GSK3{beta}) and 70 kDa ribosomal S6 kinase (p70S6K). The treatment of MDA-MB-231 cells with MG132 induced endoplasmic reticulum stress through the induction of ATF6a, PERK phosphorylation, and CHOP, and apoptosis through the cleavage of Bax and procaspase-3. MG132 caused the phosphorylation of GSK3{beta} at Ser{sup 9} and, to a lesser extent, Thr{sup 390}, the dephosphorylation of p70S6K at Thr{sup 389}, and the phosphorylation of p70S6K at Thr{sup 421} and Ser{sup 424}. The specific p38 inhibitor SB203080 reduced the p-GSK3{beta}{sup Ser9} and autophagy through the phosphorylation of p70S6K{sup Thr389}; however, it augmented the levels of p-ERK, p-GSK3{beta}{sup Thr390}, and p-70S6K{sup Thr421/Ser424} induced by MG132, and increased apoptotic cell death. The GSK inhibitor SB216763, but not lithium, inhibited the MG132-induced phosphorylation of p38, and the downstream signaling pathway was consistent with that in SB203580-treated cells. Taken together, our data show that proteasome inhibition regulates p38/GSK{sup Ser9}/p70S6K{sup Thr380} and ERK/GSK3{beta}{sup Thr390}/p70S6K{sup Thr421/Ser424} kinase signaling, which is involved in cell survival and cell death.

  1. Comparison of Petrophysical Rock Types from Core and Well-logs using Post-stack 3D Seismic Data: Field Example from Maracaibo-Venezuela

    E-Print Network [OSTI]

    Ramachandran, Kumar

    : Field Example from Maracaibo-Venezuela Francisco Cheng* and Kumar Ramachandran, The University of Tulsa in an area located on the West side of Lake Maracaibo-Venezuela. The calculated petrophysical rock types were Description The study area is located on the West side of Lake Maracaibo-Venezuela, in shallow, transition

  2. To appear in the Proceedings of the World Multiconference on Systemics, Cybernetics, and Informatics (CSI'97), Caracas, Venezuela, July 1997. Learning of ParameterAdaptive Reactive Controllers for Robotic

    E-Print Network [OSTI]

    , and Informatics (CSI'97), Caracas, Venezuela, July 1997. Learning of Parameter­Adaptive Reactive Controllers

  3. Regulation XVI: GENERAL UNIVERSITY REGULATIONS

    E-Print Network [OSTI]

    Regulation XVI: GENERAL UNIVERSITY REGULATIONS APPLICATION AND INTERPRETATION 1. Unless stated otherwise, these and the following Regulations apply to students in all Faculties, including the International Faculty: General Regulations for First Degrees; General Regulations for Higher Degrees

  4. Misión Madres Del Barrio: A Bolivarian Social Program Recognizing Housework and Creating A Caring Economy in Venezuela

    E-Print Network [OSTI]

    Fischer-Hoffman, Cory

    2008-08-20

    ABSTRACT: This thesis began as a project about Misión Madres del Barrio; and it quickly transformed into a glimpse of the Bolivarian Revolution underway in Venezuela. Misión Madres del Barrio (MMB) is one of the pioneering social programs...

  5. Risk prevention and policy formulation : responding to the 1999 mud-floods catastrophe in El Litoral Central, Venezuela

    E-Print Network [OSTI]

    Parisca-Blanco, Sonia

    2005-01-01

    Fifteen days of constant and intense rainfall in Venezuela culminated on December 16 1999, in catastrophic landslides and flooding along 25 miles of the Vargas State coastal strip. This catastrophe ravaged the Caracas ...

  6. Reservoir characterization of the upper Merecure and lower Oficina Formations sands in the Leona Este Field, Eastern Venezuela Basin 

    E-Print Network [OSTI]

    Flores Millan, Maria Carolina

    2001-01-01

    Field, which is located in the southern portion of the Eastern Venezuela Basin. Two or more of these reservoir sands, which are interbedded with shales, have been simultaneously produced pursuing an increase in the oil production rate, but an unexpected...

  7. Household energy use in urban Venezuela: Implications from surveys in Maracaibo, Valencia, Merida, and Barcelona-Puerto La Cruz

    SciTech Connect (OSTI)

    Figueroa, M.J.; Sathaye, J.

    1993-08-01

    This report identifies the most important results of a comparative analysis of household commercial energy use in Venezuelan urban cities. The use of modern fuels is widespread among all cities. Cooking consumes the largest share of urban household energy use. The survey documents no use of biomass and a negligible use of kerosene for cooking. LPG, natural gas, and kerosene are the main fuels available. LPG is the fuel choice of low-income households in all cities except Maracaibo, where 40% of all households use natural gas. Electricity consumption in Venezuela`s urban households is remarkably high compared with the levels used in households in comparable Latin American countries and in households of industrialized nations which confront harsher climatic conditions and, therefore, use electricity for water and space heating. The penetration of appliances in Venezuela`s urban households is very high. The appliances available on the market are inefficient, and there are inefficient patterns of energy use among the population. Climate conditions and the urban built form all play important roles in determining the high level of energy consumption in Venezuelan urban households. It is important to acknowledge the opportunities for introducing energy efficiency and conservation in Venezuela`s residential sector, particularly given current economic and financial constraints, which may hamper the future provision of energy services.

  8. Estradiol-Induced Object Memory Consolidation in Middle-Aged Female Mice Requires Dorsal Hippocampal Extracellular Signal-Regulated Kinase and Phosphatidylinositol 3-Kinase Activation

    E-Print Network [OSTI]

    Lewis, Michael C.

    We previously demonstrated that dorsal hippocampal extracellular signal-regulated kinase (ERK) activation is necessary for 17?[beta]-estradiol (E2[E subscript 2]) to enhance novel object recognition in young ovariectomized ...

  9. FRONTISPIECE. Three-striped Warblers (Basileuterus tristriatus) were studied in the northern Andes of Venezuela. Temperate and tropical parulids differ strongly in life histories. Three-striped Warblers have smaller clutches, longer

    E-Print Network [OSTI]

    Martin, Thomas E.

    of Venezuela. Temperate and tropical parulids differ strongly in life histories. Three-striped Warblers have(4):667­678, 2009 BREEDING BIOLOGY OF THE THREE-STRIPED WARBLER IN VENEZUELA: A CONTRAST BETWEEN TROPICAL traits of the Three-striped Warbler (Basileuterus tristriatus) from 146 nests in Venezuela and compare

  10. Lysyl oxidase like 4, a novel target gene of TGF-{beta}1 signaling, can negatively regulate TGF-{beta}1-induced cell motility in PLC/PRF/5 hepatoma cells

    SciTech Connect (OSTI)

    Kim, Dong Joon [Medical Genomics Research Center, KRIBB, P.O. Box 115, Yusong, Daejeon 305-806 (Korea, Republic of); College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Lee, Dong Chul; Yang, Suk-Jin; Lee, Jung Ju; Bae, Eun Mi; Kim, Dong Min; Min, Sang Hyun; Kim, Soo Jung [Medical Genomics Research Center, KRIBB, P.O. Box 115, Yusong, Daejeon 305-806 (Korea, Republic of); Kang, Dong Chul [Ilsong Institute of Life Science, Hallym University, Anyang 431-060 (Korea, Republic of); Sang, Byung Chan [College of Agricultural Life and Sciences, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Myung, Pyung Keun [College of Pharmacy, Chungnam National University, Daejeon 305-764 (Korea, Republic of); Park, Kyung Chan [Medical Genomics Research Center, KRIBB, P.O. Box 115, Yusong, Daejeon 305-806 (Korea, Republic of)], E-mail: kpark@kribb.re.kr; Yeom, Young Il [Medical Genomics Research Center, KRIBB, P.O. Box 115, Yusong, Daejeon 305-806 (Korea, Republic of)], E-mail: yeomyi@kribb.re.kr

    2008-09-05

    Transforming growth factor-{beta}1 (TGF-{beta}1) is a multi-functional cytokine involved in the regulation of cell proliferation, differentiation and extracellular matrix formation. In search for novel genes mediating the TGF-{beta}1 function at downstream signaling, we performed a cDNA microarray analysis and identified 60 genes whose expression is regulated by TGF-{beta}1 in the liver cancer cell line PLC/PRF/5. Among them, we report here lysyl oxidase like 4 (LOXL4) as a novel target of TGF-{beta}1 signaling, and provide experimental evidence for its expression regulation and function. LOXL4 was found to be the only member of LOX family whose expression is induced by TGF-{beta}1 in hepatoma cells. Deletion mapping of the LOXL4 promoter indicated that the TGF-{beta}1 regulation of LOXL4 expression is mediated through the binding of AP1 transcription factor to a conserved region of the promoter. This was confirmed by the chromatin immunoprecipitation assay that captured c-Fos-bound chromatin from TGF-{beta}1-treated cells. Forced expression of LOXL4 in PLC/PRF/5 cells resulted in inhibition of cell motility through Matrigel in the presence of TGF-{beta}1 treatment. In parallel, LOXL4 suppressed the expression of laminins and {alpha}3 integrin and the activity of MMP2. These results suggest that LOXL4 may function as a negative feedback regulator of TGF-{beta}1 in cell invasion by inhibiting the metabolism of extracellular matrix (ECM) components.

  11. Fertility and rate of growth in a beef cattle herd in the 0rient of Venezuela 

    E-Print Network [OSTI]

    Gonzalez Crespo, Justo Nicolas

    1978-01-01

    status upon pregnancy rate in Venezuela. They calculated adjusted means of lactating and nonlactating cows to be 54. 3~~ and 89. 2~ respect. ively. Such results have been re- ported by . , everal authors in Bolivia (Plasse et aL. , 1975 and Bauer et a... and Huertas, 1974, in Colombia and Bauer, 1973, in Bolivia). Thc negative effect of lactation upon ferti. lity seems to be related to the plane of nutrition (IViltbank et al. , 1961, 1964; Villar et al, 1975; Stonaker et al, 1975 and Bazan et al. , 1975...

  12. Involvement of a chromatin modifier in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell injury: Probably an indirect action via the regulation of NF?B/FasL circuitry

    SciTech Connect (OSTI)

    Chen, Shiwei [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China)] [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China); Dong, Yushu [Department of Neurosurgery, 463rd Hospital of PLA, Shenyang 110042 (China)] [Department of Neurosurgery, 463rd Hospital of PLA, Shenyang 110042 (China); Xu, Chun; Jiang, Liming; Chen, Yongjie; Jiang, Cheng [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China)] [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China); Hou, Wugang, E-mail: gangwuhou@163.com [Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032 (China)] [Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032 (China); Li, Wei, E-mail: liweipepeyato@163.com [Department of Human Anatomy, Histology and Embryology, Fourth Military Medical University, Xi’an 710032 (China)] [Department of Human Anatomy, Histology and Embryology, Fourth Military Medical University, Xi’an 710032 (China)

    2013-11-01

    Highlights: •MTA1 expression is upregulated in SCs upon MEHP treatment. •Knockdown of MTA1 in SCs impairs the MEHP-induced NF?B signaling activation. •Knockdown of MTA1 inhibits recruitment of NF?B onto FasL promoter in MEHP-treated SCs. -- Abstract: The Fas/FasL signaling pathway, controlled by nuclear factor-?B (NF?B) at the transcriptional level, is critical for triggering germ cell apoptosis in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell (SC) injury, but the exact regulation mechanism remain unknown. Here, we discovered that expression level of Metastasis associated protein 1 (MTA1), a component of the Mi-2/nucleosome remodeling and deacetylase complex, was upregulated in SCs during the early recovery after MEHP exposure. This expression change was in line with the dynamic changes in germ cell apoptosis in response to MEHP treatment. Furthermore, a knockdown of MTA1 by RNAi in SCs was found to impair the MEHP-induced early activation of NF?B pathway and abolish the recruitment of NF?B onto FasL promoter, which consequently diminished the MEHP-triggered FasL induction. Considering that Fas/FasL is a well characterized apoptosis initiating signaling during SCs injury, our results point to a potential “switch on” effect of MTA1, which may govern the activation of NF?B/FasL cascade in MEHP-insulted SCs. Overall, the MTA1/NF?B/FasL circuit may serve as an important defensive/repairing mechanism to help to control the germ cell quality after SCs injury.

  13. Settlement patterns in the eastern coast of Maracaibo Lake, Venezuela : evaluation of existing settlements and model for a low income sector of El Menito new town

    E-Print Network [OSTI]

    Rodriguez Navas, Humberto José

    1983-01-01

    This thesis is divided in two parts; The First part is the physical analysis of residential settlements in the Eastern Coast of Maracaibo Lake, Venezuela. The work is based on surveys, evaluations and comparisons of four ...

  14. Training Hybrid Neuro-Fuzzy System to Infer Permeability in Wells on Maracaibo Lake, Venezuela

    E-Print Network [OSTI]

    Hurtado, Nuri; Torres, Julio

    2014-01-01

    The high accuracy on inferrring of rocks properties, such as permeability ($k$), is a very useful study in the analysis of wells. This has led to development and use of empirical equations like Tixier, Timur, among others. In order to improve the inference of permeability we used a hybrid Neuro-Fuzzy System (NFS). The NFS allowed us to infer permeability of well, from data of porosity ($\\phi$) and water saturation ($Sw$). The work was performed with data from wells VCL-1021 (P21) and VCL-950 (P50), Block III, Maracaibo Lake, Venezuela. We evaluated the NFS equations ($k_{P50,i}(\\phi_i,Sw_i)$) with neighboring well data ($P21$), in order to verify the validity of the equations in the area. We have used ANFIS in MatLab.

  15. Regulation XVII: GENERAL REGULATIONS FOR FIRST DEGREES

    E-Print Network [OSTI]

    Regulation XVII: GENERAL REGULATIONS FOR FIRST DEGREES SCOPE OF THESE REGULATIONS4 1. These Regulations apply, subject to any different provision in the Regulations for a particular programme of study programme of study is designated as a non-modular programme, Regulation 14 and subsequent Regulations

  16. Government Regulation

    E-Print Network [OSTI]

    Ashford, Nicholas

    2005-01-01

    Abstract. Interest in the use of so-called voluntary approaches to supplement or replace formal environmental regulation is on the rise, both in Europe and in the United States. These approaches fall into two general ...

  17. Uptake of donor lymphocytes treated with 8-methoxypsoralen and ultraviolet A light by recipient dendritic cells induces CD4{sup +}CD25{sup +}Foxp3{sup +} regulatory T cells and down-regulates cardiac allograft rejection

    SciTech Connect (OSTI)

    Zheng, De-Hua; Dou, Li-Ping; Wei, Yu-Xiang; Du, Guo-Sheng; Zou, Yi-Ping; Song, Ji-Yong; Zhu, Zhi-Dong; Cai, Ming; Qian, Ye-Yong; Shi, Bing-Yi

    2010-05-14

    Extracorporeal photopheresis (ECP) is an effective immunomodulatory therapy and has been demonstrated to be beneficial for graft-vs-host disease and solid-organ allograft rejection. ECP involves reinfusion of a patient's autologous peripheral blood leukocytes treated ex vivo with 8-methoxypsoralen and UVA light radiation (PUVA). Previous studies focused only on ECP treatment of recipient immune cells. Our study is the first to extend the target of ECP treatment to donor immune cells. The results of in vitro co-culture experiments demonstrate uptake of donor PUVA-treated splenic lymphocytes (PUVA-SPs) by recipient immature dendritic cells (DCs). Phagocytosis of donor PUVA-SPs does not stimulate phenotype maturation of recipient DCs. In the same co-culture system, donor PUVA-SPs enhanced production of interleukin-10 and interferon-{gamma} by recipient DCs and impaired the subsequent capability of recipient DCs to stimulate recipient naive T cells. Phagocytosis of donor PUVA-SP (PUVA-SP DCs) by recipient DCs shifted T-cell responses in favor of T helper 2 cells. Infusion of PUVA-SP DCs inhibited cardiac allograft rejection in an antigen-specific manner and induced CD4{sup +}CD25{sup high}Foxp3{sup +} regulatory T cells. In conclusion, PUVA-SP DCs simultaneously deliver the donor antigen and the regulatory signal to the transplant recipient, and thus can be used to develop a novel DC vaccine for negative immune regulation and immune tolerance induction.

  18. Regulation Theory

    E-Print Network [OSTI]

    Bouvet, F

    2015-01-01

    This paper reviews the design of regulation loops for power converters. Power converter control being a vast domain, it does not aim to be exhaustive. The objective is to give a rapid overview of the main synthesis methods in both continuous- and discrete-time domains.

  19. Regulation 29: Other Regulations concerning the University site and buildings, Computing Regulations and Miscellaneous Administrative Regulations

    E-Print Network [OSTI]

    Sussex, University of

    Regulation 29: Other Regulations concerning the University site and buildings, Computing Regulations and Miscellaneous Administrative Regulations 190 nuisance to occupants, except in designated (Vehicle Operators and Penalty Notices) Regulations; (iii) allow smoking in designated study bedrooms

  20. Regulation XVIII: GENERAL REGULATIONS FOR HIGHER DEGREES,

    E-Print Network [OSTI]

    Regulation XVIII: GENERAL REGULATIONS FOR HIGHER DEGREES, POSTGRADUATE DIPLOMAS AND POSTGRADUATE CERTIFICATES SCOPE OF THESE REGULATIONS 1. These Regulations apply to the Degree of PhD in all Faculties in all Faculties Postgraduate Certificates in all Faculties. 2. These Regulations are subject

  1. Regulation 28: Library REGULATION 28: LIBRARY

    E-Print Network [OSTI]

    Sussex, University of

    Regulation 28: Library 180 REGULATION 28: LIBRARY The purpose of this Regulation is to safeguard the common interests of all Library users. All persons are admitted on the understanding that they have read and agreed to observe the Library Regulations. Breach of this Regulation could result in membership being

  2. A study of beef cattle marketing in Venezuela and the marketing margins between the farm and retail levels of prices 

    E-Print Network [OSTI]

    Acosta, Sady Ines Borjas-Paez

    1975-01-01

    partial fulf i 1 lment of the recu'. rement i or rhe degree of MASTEF, OF SCIENCE December 1975 Major Subject: Agricultural Economics A STUDY OF B EF' CATTLE M. 'RKEl'lNG :N VENEZUELA AND THE MARKETING MAPGINS BETWEEN THE FVARM AND RETAIL LEVELS Ot... for the period 1960-1972 were studied from the farm through 'retail levels, the variations in price spread were large. Lack of knowledge concerning share of marketing margins has brought inadequate govern- ment policies. The structural demand equation...

  3. Charge regulation circuit

    DOE Patents [OSTI]

    Ball, Don G. (Livermore, CA)

    1992-01-01

    A charge regulation circuit provides regulation of an unregulated voltage supply in the range of 0.01%. The charge regulation circuit is utilized in a preferred embodiment in providing regulated voltage for controlling the operation of a laser.

  4. Hypsilara royi gen. n. and sp. n. (Coleoptera, Elmidae, Larainae) from Southern Venezuela, with a revised key to Larainae of the Western Hemisphere

    E-Print Network [OSTI]

    Maier, Crystal A.; Spangler, Paul J.

    2011-07-07

    Here we describe a new genus, for a new species of riffle beetle, Hypsilara royi gen. n. and sp. n., from the tepui Cerro de la Neblina in southern Venezuela. This new genus can be distinguished from all other laraine genera by its small size (ca. 4...

  5. Venezuela-MEM/USA-DOE Fossil Energy Report IV-11: Supporting technology for enhanced oil recovery - EOR thermal processes

    SciTech Connect (OSTI)

    Venezuela

    2000-04-06

    This report contains the results of efforts under the six tasks of the Tenth Amendment anti Extension of Annex IV, Enhanced Oil Recovery Thermal Processes of the Venezuela/USA Energy Agreement. This report is presented in sections (for each of the six Tasks) and each section contains one or more reports that were prepared to describe the results of the effort under each of the Tasks. A statement of each Task, taken from the Agreement Between Project Managers, is presented on the first page of each section. The Tasks are numbered 68 through 73. The first through tenth report on research performed under Annex IV Venezuela MEM/USA-DOE Fossil Energy Report Number IV-1, IV-2, IV-3, IV-4, IV-5, IV-6, IV-7, IV-8, IV-9, IV-10 contain the results of the first 67 Tasks. These reports are dated April 1983, August 1984, March 1986, July 1987, November 1988, December 1989, October 1991, February 1993, March 1995, and December 1997, respectively.

  6. Modelling regulations Completing an incomplete regulation

    E-Print Network [OSTI]

    van der Torre, Leon

    Objectives Modelling regulations Completing an incomplete regulation Examples Discussion Consistency and Completeness of Regulations Laurence Cholvy1 St´ephanie Roussel1,2 1ONERA Centre de Toulouse 2ISAE, Toulouse NorMAS 2008, Luxembourg, July 2008 cholvy Consistency and Completeness of Regulations

  7. Studies on gene expression profiling in JB6 cells susceptible and resistant to tumor promoter induced neoplastic transformation and regulation of gene expression at the AP-1 DNA binding site 

    E-Print Network [OSTI]

    Samuel, Shaija

    2005-11-01

    Gene expression underlies all important biological processes in a cell and mis-regulated gene expression plays a causal or contributory role in several diseases including cancers. Towards identifying molecular determinants ...

  8. Regulators, Requirements, Statutes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regulations and the New Mexico Administrative Code. Federal Requirements The Environmental Protection Agency (EPA) enforces the federal regulations. Code of Federal...

  9. Tryptophan Regulation of the Escherichia coli Tryptophanase (TNA) Operon 

    E-Print Network [OSTI]

    Martinez, Allyson Kendall

    2013-10-22

    Free L-tryptophan induces the expression of the Escherichia coli tna operon that specifies proteins necessary for catabolizing tryptophan. Regulation is effected by a transcriptional attenuation mechanism requiring ...

  10. Natural Gas Regulations (Kentucky)

    Broader source: Energy.gov [DOE]

    Kentucky Administrative Regulation title 805 promulgates the rules and regulations pertaining to natural gas production in Kentucky. In addition to KAR title 405, chapter 30, which pertains to any...

  11. Emotion Regulation CONCEPTUAL FOUNDATIONS

    E-Print Network [OSTI]

    Gross, James J.

    CHAPTER 1 Emotion Regulation CONCEPTUAL FOUNDATIONS JAMES J. GROSS ROSS A. THOMPSON Standing, paper or plastic are made. Quotidian acts of emotion regulation such as this constitute one important- changes that require us to regulate how emotions are experienced and expressed. But what do people do

  12. 3 Library Regulations Definitions

    E-Print Network [OSTI]

    Mottram, Nigel

    3 Library Regulations Definitions In Regulation 3: 'Library' means the University Library as defined in Regulation 3.1; 'Library staff' means the staff of the University Library; 'Librarian' means the University Librarian and Head of Information Resources Directorate or nominee; `Library Committee' means

  13. Approaches to identifying reservoir heterogeneity and reserve growth opportunities from subsurface data: The Oficina Formation, Budare field, Venezuela

    SciTech Connect (OSTI)

    Hamilton, D.S.; Raeuchle, S.K.; Holtz, M.H.

    1997-08-01

    We applied an integrated geologic, geophysical, and engineering approach devised to identify heterogeneities in the subsurface that might lead to reserve growth opportunities in our analysis of the Oficina Formation at Budare field, Venezuela. The approach involves 4 key steps: (1) Determine geologic reservoir architecture; (2) Investigate trends in reservoir fluid flow; (3) Integrate fluid flow trends with reservoir architecture; and (4) Estimate original oil-in-place, residual oil saturation, and remaining mobile oil, to identify opportunities for reserve growth. There are three main oil-producing reservoirs in the Oficina Formation that were deposited in a bed-load fluvial system, an incised valley-fill, and a barrier-strandplain system. Reservoir continuity is complex because, in addition to lateral facies variability, the major Oficina depositional systems were internally subdivided by high-frequency stratigraphic surfaces. These surfaces define times of intermittent lacustrine and marine flooding events that punctuated the fluvial and marginal marine sedimentation, respectively. Syn and post depositional faulting further disrupted reservoir continuity. Trends in fluid flow established from initial fluid levels, response to recompletion workovers, and pressure depletion data demonstrated barriers to lateral and vertical fluid flow caused by a combination of reservoir facies pinchout, flooding shale markers, and the faults. Considerable reserve growth potential exists at Budare field because the reservoir units are highly compartment by the depositional heterogeneity and structural complexity. Numerous reserve growth opportunities were identified in attics updip of existing production, in untapped or incompletely drained compartments, and in field extensions.

  14. Load regulating expansion fixture

    DOE Patents [OSTI]

    Wagner, L.M.; Strum, M.J.

    1998-12-15

    A free standing self contained device for bonding ultra thin metallic films, such as 0.001 inch beryllium foils is disclosed. The device will regulate to a predetermined load for solid state bonding when heated to a bonding temperature. The device includes a load regulating feature, whereby the expansion stresses generated for bonding are regulated and self adjusting. The load regulator comprises a pair of friction isolators with a plurality of annealed copper members located therebetween. The device, with the load regulator, will adjust to and maintain a stress level needed to successfully and economically complete a leak tight bond without damaging thin foils or other delicate components. 1 fig.

  15. Load regulating expansion fixture

    DOE Patents [OSTI]

    Wagner, Lawrence M. (San Jose, CA); Strum, Michael J. (San Jose, CA)

    1998-01-01

    A free standing self contained device for bonding ultra thin metallic films, such as 0.001 inch beryllium foils. The device will regulate to a predetermined load for solid state bonding when heated to a bonding temperature. The device includes a load regulating feature, whereby the expansion stresses generated for bonding are regulated and self adjusting. The load regulator comprises a pair of friction isolators with a plurality of annealed copper members located therebetween. The device, with the load regulator, will adjust to and maintain a stress level needed to successfully and economically complete a leak tight bond without damaging thin foils or other delicate components.

  16. How nutrients, energy state, and hormones reprogram metabolism : transcriptional regulators controlled by the LKB1/AMPK pathway

    E-Print Network [OSTI]

    Mihaylova, Maria Milkova

    2012-01-01

    Acetylation Regulates Gluconeogenesis by Promoting PEPCK1Induced Hepatic Gluconeogenesis. Diabetes 61, 513- 523.switch modulates gluconeogenesis via activator/coactivator

  17. Emotion Regulation and Vulnerability to Depression: Spontaneous Versus Instructed Use of Emotion Suppression and Reappraisal

    E-Print Network [OSTI]

    Gross, James J.

    Emotion Regulation and Vulnerability to Depression: Spontaneous Versus Instructed Use of Emotion vulnerability is related to difficulties with emotion regulation by comparing recovered-depressed and never of emotion regulation strategies. In the second phase, sad mood was induced using a film clip, and the degree

  18. Pressure reducing regulator

    DOE Patents [OSTI]

    Whitehead, John C. (Davis, CA); Dilgard, Lemoyne W. (Willits, CA)

    1995-01-01

    A pressure reducing regulator that controls its downstream or outlet pressure to a fixed fraction of its upstream or inlet pressure. The regulator includes a housing which may be of a titanium alloy, within which is located a seal or gasket at the outlet end which may be made of annealed copper, a rod, and piston, each of which may be made of high density graphite. The regulator is insensitive to temperature by virtue of being without a spring or gas sealed behind a diaphragm, and provides a reference for a system in which it is being used. The rod and piston of the regulator are constructed, for example, to have a 1/20 ratio such that when the downstream pressure is less than 1/20 of the upstream pressure the regulator opens and when the downstream pressure exceeds 1/20 of the upstream pressure the regulator closes.

  19. Pressure reducing regulator

    DOE Patents [OSTI]

    Whitehead, J.C.; Dilgard, L.W.

    1995-10-10

    A pressure reducing regulator that controls its downstream or outlet pressure to a fixed fraction of its upstream or inlet pressure is disclosed. The regulator includes a housing which may be of a titanium alloy, within which is located a seal or gasket at the outlet end which may be made of annealed copper, a rod, and piston, each of which may be made of high density graphite. The regulator is insensitive to temperature by virtue of being without a spring or gas sealed behind a diaphragm, and provides a reference for a system in which it is being used. The rod and piston of the regulator are constructed, for example, to have a 1/20 ratio such that when the downstream pressure is less than 1/20 of the upstream pressure the regulator opens and when the downstream pressure exceeds 1/20 of the upstream pressure the regulator closes. 10 figs.

  20. Cenozoic oblique collision of South American and Caribbean plates: New evidence in the Coastal Cordillera of Venezuela and Trinidad

    SciTech Connect (OSTI)

    Speed, R.C. (Northwestern Univ., Evanston, IL (United States)); Russo, R.M. (Carnegie Institute of Washington (United States)); Foland, K.A. (Ohio State Univ., Columbus (United States))

    1993-02-01

    The hinterland of the Caribbean Mts. orogen in Trinidad and Venezuela contains schist and gneiss whole protoliths are wholly or partly of continental provenance. The hinterland lies between the foreland thrust belt and terranes. The terranes are alien to continental South America (SA) and may have proto-Caribbean or Caribbean plate origins. The hinterland rocks were widely thought to come from sediments and granitoids of Mesozoic protolithic ages and to be of Cretaceous metamorphic age. Such rocks are now know to be of at least two or more types, as follows: (1) low grade, protoliths of pre-Mesozoic basement and shelfal cover of uncertain age range, inboard locus, Oligocene to mid-Miocene metamorphic ages younging eastward (Caracas, Paria, and Northern Range belts), and (2) higher grade including high P/T, varies protoliths of uncertain age range, Cretaceous and ( )early Paleogene metamorphic ages (Tacagua, Araya, Margarita). The geometry, protoliths, structures, and metamorphic ages of type 1 parautochthoneity and an origin as a thickened wedge of crust-cored passive margin cover. The wedge grew by accretion between about 35 and 20 Ma during oblique transport toward the foreland. The diachroneity of metamorphism implies, as does the timing of foreland deformation, that the wedge evolved in a right-oblique collision between northern SA and terranes moving wholly or partly with the Caribbean plate since the Eocene. Type 2 rocks probably came with the terranes and are products of convergent zone tectonics, either in the proto-Caribbean plate. The hinterland boundaries are brittle thrusts that are out of sequence and imply progressive contraction from mid-Cenozoic to the present.

  1. Resources for Utility Regulators

    SciTech Connect (OSTI)

    SEE Action

    2012-06-01

    Provides a summary of State and Local Energy Efficiency Action Network (SEE Action) information resources available to utility regulators, organized by topic.

  2. Academic Regulations Academic Regulations and University Policies

    E-Print Network [OSTI]

    Fletcher, Robin

    dignity of all persons" depends on an adherence to academic integrity in all its actions. In support An academic community of integrity upholds personal accountability and depends upon action in the face of the Academic Regulations with references to University Policies: 1 Academic Integrity 2 Enrolment

  3. REGULATION I: Responsibility for Creation and Amendment of Regulations

    E-Print Network [OSTI]

    REGULATION I: Responsibility for Creation and Amendment of Regulations 1. In accordance with Article 14 of the Charter, the Council shall have the power to make, amend or repeal Regulations. 2 of the Regulations and the delegation of such power pursuant to Regulation II (7.2) by the Council to Senate

  4. Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems

    SciTech Connect (OSTI)

    Majer, Ernie; Nelson, James; Robertson-Tait, Ann; Savy, Jean; Wong, Ivan

    2012-01-01

    This Protocol is a living guidance document for geothermal developers, public officials, regulators and the general public that provides a set of general guidelines detailing useful steps to evaluate and manage the effects of induced seismicity related to EGS projects.

  5. Post-translational regulation enables robust p53 regulation

    E-Print Network [OSTI]

    Shin, Yong-Jun; Chen, Kai-Yuan; Sayed, Ali H; Hencey, Brandon; Shen, Xiling

    2013-01-01

    PS, Elowitz MB: Gene regulation at the single-cell level.JC, Song B, Kudo K, Chu E: Regulation of p53 expression inChen L, Li Z, et al: Regulation of MDM2 E3 ligase activity

  6. Army Regulation 690600 Civilian Personnel

    E-Print Network [OSTI]

    US Army Corps of Engineers

    Army Regulation 690­600 Civilian Personnel Equal Employment Opportunity Discrimination Complaints Civilian Personnel Equal Employment Opportunity Discrimination Complaints *Army Regulation 690­600 Effective 9 March 2004 History. This publication is a major revision. Summary. This regulation establishes

  7. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit...

  8. Federal Acquisition Regulation; Federal Acquisition Circular...

    Energy Savers [EERE]

    Federal Acquisition Regulation; Federal Acquisition Circular Federal Acquisition Regulation; Federal Acquisition Circular Federal Acquisition Regulation; Federal Acquisition...

  9. North America: Regulation of International Electricity Trade...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    North America: Regulation of International Electricity Trade North America: Regulation of International Electricity Trade North America: Regulation of International Electricity...

  10. Federal Acquisition Regulation; Federal Acquisition Circular...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation; Federal Acquisition Circular Federal Acquisition Regulation; Federal Acquisition Circular Federal Acquisition Regulation; Federal Acquisition Circular More Documents &...

  11. Regulation 2: Student Discipline REGULATION 2: STUDENT DISCIPLINE

    E-Print Network [OSTI]

    Sussex, University of

    Regulation 2: Student Discipline 6 REGULATION 2: STUDENT DISCIPLINE 1. Definitions In this Regulation: The University means the University of Sussex. Council means Council of the University. Senate to the regulations of the affiliated institution, and excluding students in attendance at the Brighton and Sussex

  12. Dirty and perverse: regulation-induced pollution substitution

    E-Print Network [OSTI]

    Gibson, Matthew

    2014-01-01

    plants increase their ratio of water to air emissions by 42plants increase their ratio of water to air emissions by 42an increase in the ratio of water to air pollution V%*. This

  13. Estrogen induced {beta}-1,4-galactosyltransferase 1 expression regulates

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article) | SciTech(Journal(Patent)pressure in Ba ( Fe 1 - x Coproliferation of

  14. MINIREVIEW / MINISYNTHE` SE The role of chromatin structure in regulating

    E-Print Network [OSTI]

    Krebs, Jocelyn E.

    within many stress-induced promoters, such as stress re- sponse elements and heat shock elements, allow. Key words: chromatin, transcription, stress response, heat shock. Re´sume´ : Toutes les cellules, quMINIREVIEW / MINISYNTHE` SE The role of chromatin structure in regulating stress

  15. Regulation of Glycosaminoglycan Function by Osmotic Potentials MEASUREMENT OF WATER TRANSFER DURING ANTITHROMBIN ACTIVATION BY HEPARIN*

    E-Print Network [OSTI]

    Dai, Yang

    Regulation of Glycosaminoglycan Function by Osmotic Potentials MEASUREMENT OF WATER TRANSFER DURING glycosaminoglycans can be regulated by osmotic potentials. Volume of water trans- ferred upon heparin binding was measured from differ- ences in free energy change, ( G), with osmotic stress, . Osmotic stress was induced

  16. Proactive Price Regulation for Upgrading Telecommunications Infrastructure and Jon M. Peha

    E-Print Network [OSTI]

    Peha, Jon M.

    1 Proactive Price Regulation for Upgrading Telecommunications Infrastructure Qiong Wang 1 and Jon M that upgrade occurs. This paper discusses a proactive pricing approach in which the regulator defines a pricing, and specify a proactive pricing policy to induce that timing. We also demonstrate a tradeoff between promoting

  17. Design of Optimal Regulators

    E-Print Network [OSTI]

    Alexander Bolonkin; Robert Sierakowski

    2007-01-19

    Current research suggests the use of a liner quadratic performance index for optimal control of regulators in various applications. Some examples include correcting the trajectory of rocket and air vehicles, vibration suppression of flexible structures, and airplane stability. In all these cases, the focus is in suppressing/decreasing system deviations rapidly. However, if one compares the Linear Quadratic Regulator (LQR) solution with optimal solutions (minimum time), it is seen that the LQR solution is less than optimal in some cases indeed (3-6) times that obtained using a minimum time solution. Moreover, the LQR solution is sometimes unacceptable in practice due to the fact that values of control extend beyond admissible limits and thus the designer must choose coefficients in the linear quadratic form, which are unknown. The authors suggest methods which allow finding a quasi-optimal LQR solution with bounded control which is closed to the minimum time solution. They also remand the process of the minimum time decision. Keywords: Optimal regulator, minimum time controller, Linear Quadratic Regulator (LQR). -- This paper is declared a work of the U.S. Government and not subject to copyright protection in the USA. The manuscript is accepted as paper AIAA-2003-6638 by 2nd AIAA Unmanned Unlimited Systems, Technologies, and Operations-Aerospace, Land, and See Conference and Workshop - Exhibit, San Diego, California, USA, 15-18 Sep. 2003.

  18. Cascaded'' pilot regulators help reduce LPG loss in hot weather

    SciTech Connect (OSTI)

    Not Available

    1994-08-08

    Fina Oil and Chemical Co. and Fisher Controls International used engineering resourcefulness to overcome heat-induced product loss from LPG storage bullets at Fina's Port Arthur, Tex., refinery. Fina had installed Fisher's Easy Joe 399A-6365, a pilot-operated, back-pressure-type regulator, on its LPG storage facility in 1991 as part of a fuel products modernization project. The regulators helped control the accumulation of noncondensible vapors, which collect in the storage bullets above the LPG. But summer heat extremes and surges in the tanks and lines made it possible for the operating pressure to increase so that the safety relief valve was activated before the pilot regulator was able to stabilize the pressure. The installation of pilot-type regulators, in cascaded, or series, formation, reduced product venting through relief valves.

  19. Regulation of Meiotic Recombination

    SciTech Connect (OSTI)

    Gregory p. Copenhaver

    2011-11-09

    Meiotic recombination results in the heritable rearrangement of DNA, primarily through reciprocal exchange between homologous chromosome or gene conversion. In plants these events are critical for ensuring proper chromosome segregation, facilitating DNA repair and providing a basis for genetic diversity. Understanding this fundamental biological mechanism will directly facilitate trait mapping, conventional plant breeding, and development of genetic engineering techniques that will help support the responsible production and conversion of renewable resources for fuels, chemicals, and the conservation of energy (1-3). Substantial progress has been made in understanding the basal recombination machinery, much of which is conserved in organisms as diverse as yeast, plants and mammals (4, 5). Significantly less is known about the factors that regulate how often and where that basal machinery acts on higher eukaryotic chromosomes. One important mechanism for regulating the frequency and distribution of meiotic recombination is crossover interference - or the ability of one recombination event to influence nearby events. The MUS81 gene is thought to play an important role in regulating the influence of interference on crossing over. The immediate goals of this project are to use reverse genetics to identify mutants in two putative MUS81 homologs in the model plant Arabidopsis thaliana, characterize those mutants and initiate a novel forward genetic screen for additional regulators of meiotic recombination. The long-term goal of the project is to understand how meiotic recombination is regulated in higher eukaryotes with an emphasis on the molecular basis of crossover interference. The ability to monitor recombination in all four meiotic products (tetrad analysis) has been a powerful tool in the arsenal of yeast geneticists. Previously, the qrt mutant of Arabidopsis, which causes the four pollen products of male meiosis to remain attached, was developed as a facile system for assaying recombination using tetrad analysis in a higher eukaryotic system (6). This system enabled the measurement of the frequency and distribution of recombination events at a genome wide level in wild type Arabidopsis (7), construction of genetic linkage maps which include positions for each centromere (8), and modeling of the strength and pattern of interference (9). This proposal extends the use of tetrad analysis in Arabidopsis by using it as the basis for assessing the phenotypes of mutants in genes important for recombination and the regulation of crossover interference and performing a novel genetic screen. In addition to broadening our knowledge of a classic genetic problem - the regulation of recombination by crossover interference - this proposal also provides broader impact by: generating pedagogical tools for use in hands-on classroom experience with genetics, building interdisciplinary collegial partnerships, and creating a platform for participation by junior scientists from underrepresented groups. There are three specific aims: (1) Isolate mutants in Arabidopsis MUS81 homologs using T-DNA and TILLING (2) Characterize recombination levels and interference in mus81 mutants (3) Execute a novel genetic screen, based on tetrad analysis, for genes that regulate meiotic recombination

  20. Intrinsically Disordered Proteins: Regulation and Disease Regulation of IDPs

    E-Print Network [OSTI]

    Babu, M. Madan

    31st Jan 2011 Intrinsically Disordered Proteins: Regulation and Disease Regulation of IDPs M. Madan.....................................................................................................................................2 3.1. IDPs are tightly regulated from transcript synthesis to protein degradation ......................................................................................................................................8 #12;1 Abstract Intrinsically disordered proteins (IDPs) are often enriched in signaling

  1. Interrelationship of Program Regulations and Financial Assistance Regulations

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1980-04-24

    The purpose of this Order is to set forth the interrelationship between all program regulations which will result in assistance awards (Program Regulations) and the Department of Energy Assistance Regulations (DOE-AR, 10 CRF Part 600), including procedures for exceptions, deviations or waivers.

  2. Improving CS regulations.

    SciTech Connect (OSTI)

    Nesse, R.J.; Scheer, R.M.; Marasco, A.L.; Furey, R.

    1980-10-01

    President Carter issued Executive Order 12044 (3/28/78) that required all Federal agencies to distinguish between significant and insignificant regulations, and to determine whether a regulation will result in major impacts. This study gathered information on the impact of the order and the guidelines on the Office of Conservation and Solar Energy (CS) regulatory practices, investigated problems encountered by the CS staff when implementing the order and guidelines, and recommended solutions to resolve these problems. Major tasks accomplished and discussed are: (1) legislation, Executive Orders, and DOE Memoranda concerning Federal administrative procedures relevant to the development and analysis of regulations within CS reviewed; (2) relevant DOE Orders and Memoranda analyzed and key DOE and CS staff interviewed in order to accurately describe the current CS regulatory process; (3) DOE staff from the Office of the General Counsel, the Office of Policy and Evaluation, the Office of the Environment, and the Office of the Secretary interviewed to explore issues and problems encountered with current CS regulatory practices; (4) the regulatory processes at five other Federal agencies reviewed in order to see how other agencies have approached the regulatory process, dealt with specific regulatory problems, and responded to the Executive Order; and (5) based on the results of the preceding four tasks, recommendations for potential solutions to the CS regulatory problems developed. (MCW)

  3. Regulation 8: Responsibility for Creation and Amendment of Regulations: REGULATION 8: RESPONSIBILITY FOR CREATION AND AMENDMENT OF

    E-Print Network [OSTI]

    Sussex, University of

    Regulation 8: Responsibility for Creation and Amendment of Regulations: 36 REGULATION 8: RESPONSIBILITY FOR CREATION AND AMENDMENT OF REGULATIONS This Regulation may only be amended at a meeting and revoke Regulations. Regulations may be created, amended and revoked at any meeting of Council. 2

  4. Emotion Regulation JAMES J. GROSS

    E-Print Network [OSTI]

    Gross, James J.

    CHAPTER 31 ·Emotion Regulation JAMES J. GROSS Have you ever gotten so angry that you've done). Although the topic of emotion regulation is a relatively late addition to the field of emotion, a concern with emotion regulation is anything but new. Emotion regu lation has been a focus in the study of psycho

  5. Army Regulation 2001 Environmental Quality

    E-Print Network [OSTI]

    US Army Corps of Engineers

    Army Regulation 200­1 Environmental Quality Environmental Protection and Enhancement Headquarters Environmental Protection and Enhancement *Army Regulation 200­1 Effective 27 December 2007 History in the summary of change. Summary. This regulation covers envi- ronmental protection and enhancement and provides

  6. REGULATION AND DISTRUST Philippe AGHION

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    REGULATION AND DISTRUST Philippe AGHION Yann ALGAN Pierre CAHUC Andrei SHLEIFER January 2009 Cahier://www.enseignement.polytechnique.fr/economie/ mailto:chantal.poujouly@polytechnique.edu hal-00396268,version1-17Jun2009 #12;REGULATION AND DISTRUST1: In a cross-section of countries, government regulation is strongly negatively correlated with social capital

  7. COVARIANCE PLASTICITY AND REGULATED CRITICALITY

    E-Print Network [OSTI]

    Lehmann, Daniel

    COVARIANCE PLASTICITY AND REGULATED CRITICALITY Elie Bienenstock Division of Applied Mathematics plasticity may cause the brain to operate near criticality. We analyze the effect of such a regulation of Hebbian covariance plasticity. Such a regulation may bring the system near criticality. We suggest

  8. Temperature controlled high voltage regulator

    DOE Patents [OSTI]

    Chiaro, Jr., Peter J. (Clinton, TN); Schulze, Gerald K. (Knoxville, TN)

    2004-04-20

    A temperature controlled high voltage regulator for automatically adjusting the high voltage applied to a radiation detector is described. The regulator is a solid state device that is independent of the attached radiation detector, enabling the regulator to be used by various models of radiation detectors, such as gas flow proportional radiation detectors.

  9. Land Use Regulation with Durable Capital

    E-Print Network [OSTI]

    Quigley, John M.; Swoboda, Aaron

    2009-01-01

    Manhattan so expensive? Regulation and the rise of housingmotive for restrictive regulation by local home owners.the impacts of these regulations vary across owner- occupied

  10. Prenatal maternal stress programs infant stress regulation.

    E-Print Network [OSTI]

    Davis, Elysia Poggi; Glynn, Laura M; Waffarn, Feizal; Sandman, Curt A

    2011-01-01

    Programs Infant Stress Regulation Elysia Poggi Davis, PhDglucocorticoids disrupts the regulation of physiological andstress alters circadian regulation and laboratory levels of

  11. Price regulation for waste hauling franchises in California: an examination of how regulators regulate pricing and the effects of competition on regulated markets

    E-Print Network [OSTI]

    Seltzer, Steven A.

    2011-01-01

    Thomadakis, Stavros. “Price Regulation Under Uncertainty inin the Theory of Regulation. ” Handbook of IndustrialMark and David Sappington. “Regulation, Competition and

  12. Essays on the politics of regulation

    E-Print Network [OSTI]

    Weymouth, Stephen

    2010-01-01

    F. , 2002: Does entry regulation hinder job creation? evi-Macroeconomic effects of regulation and dereg- ulation inand Shleifer, A. , 2004: The regulation of labor. Quarterly

  13. Nanotechnology Regulation: A Study in Claims Making

    E-Print Network [OSTI]

    Malloy, Timothy F.

    2011-01-01

    Nanomaterials: Principles, Regulation, and Renegotiating theJoseph Rees, Industry Self-Regulation: An InstitutionalDarren Sinclair, Self-Regulation Versus Command and Control?

  14. Regulation, Unemployment, and Cost-Benefit Analysis

    E-Print Network [OSTI]

    Posner, Eric; Masur, Jonathan S.

    2011-01-01

    and Eric A. Posner, Regulation, Unemployment, and Cost-effects of environmental regulations for other industries.Paper Collection.   Regulation, Unemployment, and Cost-

  15. Self-regulating valve

    DOE Patents [OSTI]

    Humphreys, D.A.

    1982-07-20

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  16. Interviewee Travel Regulations Scope

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACT EVALUATION PLANIsProcessRegulationRadiative Transfer Model3/2012

  17. Aire unleashes stalled RNA polymerase to induce ectopic gene expression in thymic epithelial cells

    E-Print Network [OSTI]

    Giraud, Matthieu

    Aire is a transcriptional regulator that induces expression of peripheral tissue antigens (PTA) in thymic medullary epithelial cells (MECs), driving immunological self-tolerance in differentiating T cells. To elucidate its ...

  18. Dysregulation of nuclear factor kappa B activity and osteopontin expression in oxidant-induced atherogenesis 

    E-Print Network [OSTI]

    Williams, Edward Spencer

    2004-09-30

    NF-?B activity is critical in the regulation of atherosclerotic vascular smooth muscle cell (vSMC) phenotypes induced following oxidative injury by allylamine. The present studies were designed to detail dysregulation of NF-?B activity...

  19. Nebraska Air Quality Regulations (Nebraska)

    Broader source: Energy.gov [DOE]

    These regulations, promulgated by the Department of Environmental Quality, contain provisions pertaining to ambient air quality standards, pollution source operating permits, emissions reporting,...

  20. Environmental Regulation, Globalization, and Innovation

    E-Print Network [OSTI]

    Ashford, Nicholas

    2009-01-01

    This essay explores the complex relationship between environmental regulation, innovation, and sustainable development within the context of an increasingly globalizing economy. It will be argued that industrial policy, ...

  1. Lighting and Dark Sky Regulations

    E-Print Network [OSTI]

    Radcliffe, David

    Lighting and Dark Sky Regulations Marjorie Palmer Spring 2008 #12;The UGA Land Use Clinic provides Road, Room 101 Athens, GA 30602-1510 (706) 583-0373 · Fax (706) 583-0612 jroskie@uga.edu #12;Lighting......................................................................................................................................1 II. Approaches to Lighting Regulation

  2. REGULATION POLITIQUE ET REGULATION D'USAGE DANS LE TEMPS DE TRAVAIL1

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 REGULATION POLITIQUE ET REGULATION D'USAGE DANS LE TEMPS DE TRAVAIL1 : par G. de Terssac, J REGULATION AND REGULATION OF USE OVER WORKING TIME In this paper we discuss « social regulations » as defined by Reynaud (1999). These regulations, in our case (working)-time regulations, are multiple. Their combination

  3. Hypoxia-Inducible Factor Directs POMC Gene to Mediate Hypothalamic Glucose Sensing and Energy Balance

    E-Print Network [OSTI]

    Hayar, Abdallah

    nutrient sensing in regulation of energy and body weight balance. Citation: Zhang H, Zhang G, Gonzalez FJ Sensing and Energy Balance Regulation. PLoS Biol 9(7): e1001112. doi:10.1371/journal.pbio.1001112 AcademicHypoxia-Inducible Factor Directs POMC Gene to Mediate Hypothalamic Glucose Sensing and Energy

  4. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, Charlton (Calabasas, CA); Subbaraman, Maria R. (Canoga Park, CA)

    1989-01-01

    An improvement in an inducer for a pump wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs.

  5. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, C.; Subbaraman, M.R.

    1989-06-13

    An improvement in an inducer for a pump is disclosed wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs. 11 figs.

  6. Competitive niche: Way of population regulation

    E-Print Network [OSTI]

    Meszéna, Géza

    1 / 27 Competitive niche: Way of population regulation G´eza Mesz´ena1, Andr´as Szil´agyi1, Kalle of coexistence Theory Regulating loop Results Examples Spatial segregation Functional & spatial segregation Regulating loop y Results Examples Conclusions 7 / 27 #12;Regulating loop Introduction Theory y Regulating

  7. Output-Based Regulations: A Handbook for Air Regulators (U.S...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004 Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004 The U.S....

  8. Feedback regulation in gene networks

    E-Print Network [OSTI]

    Acar, Murat

    2007-01-01

    Cellular genetic information is encoded in DNA. The passage of this information from DNA to proteins is regulated at multiple levels and each level gives cells the chance to control the structure and function of their ...

  9. Private Regulation of Consumer Arbitration

    E-Print Network [OSTI]

    Drahozal, Christopher R.; Zyontz, Samantha

    2012-01-01

    Arbitration providers, such as the American Arbitration Association (“AAA”) and JAMS, have promulgated due process protocols to regulate the fairness of consumer and employment arbitration agreements. A common criticism of these due process...

  10. Endocrine regulation of placental phenotype

    E-Print Network [OSTI]

    Fowden, A. L.; Forhead, A. J.; Sferruzzi-Perri, A. N.; Burton, G. J.; Vaughan, O. R.

    2014-12-03

    regulates trophoblast differentiation and chorionic gonadotrophin secretion. 490 Endocrinol 2014;en20132116. 491 36 Posner BI. Insulin receptors in human and animal placental tissue. Diabetes 1974; 492 23:209-217. 493 37 Hiden U, Glitzner E, Hartmann M...

  11. Multidrug resistant protein-three gene regulation

    E-Print Network [OSTI]

    Mahaffey, Christopher M.

    2010-01-01

    H. R. ;Mulcahy, R. T. Up-regulation of the human gamma-H. R. ; Mulcahy, R. T. Regulation of gamma- glutamylcysteine19] Gerk, P. M. ; Vore, M. Regulation of expression of the

  12. MANEUVER REGULATION, TRANSVERSE FEEDBACK LINEARIZATION, AND ZERO

    E-Print Network [OSTI]

    Maggiore, Manfredi

    MANEUVER REGULATION, TRANSVERSE FEEDBACK LINEARIZATION, AND ZERO DYNAMICS Chris Nielsen,1 Manfredi focus is on output maneuver regulation where stabilizing transverse dynamics is a key requirement. Keywords: Maneuver regulation, path following, feedback linearization, zero dynamics, non-square systems

  13. Army Regulation 2550 Information Management: Records

    E-Print Network [OSTI]

    US Army Corps of Engineers

    Army Regulation 25­50 Information Management: Records Management Preparing and Managing Information Management: Records Management Preparing and Managing Correspondence *Army Regulation 25. This regulation on the prepa- ration and management of Army corre- spondence has been revised. This revision

  14. Post regulation circuit with energy storage

    DOE Patents [OSTI]

    Ball, Don G. (Livermore, CA); Birx, Daniel L. (Oakley, CA); Cook, Edward G. (Livermore, CA)

    1992-01-01

    A charge regulation circuit provides regulation of an unregulated voltage supply and provides energy storage. The charge regulation circuit according to the present invention provides energy storage without unnecessary dissipation of energy through a resistor as in prior art approaches.

  15. Searchable Electronic Department of Energy Acquisition Regulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Searchable Electronic Department of Energy Acquisition Regulation Searchable Electronic Department of Energy Acquisition Regulation Updated July 2, 2013. The EDEAR is current...

  16. The Nuclear Installations (Dangerous Occurrences) Regulations 1965 

    E-Print Network [OSTI]

    Lee, Fred; Ross, William

    1965-01-01

    STATUTORY INSTRUMENTS 1965 No. 1824 ATOMIC ENERGY AND RADIOACTIVE SUBSTANCES LICENSING AND REGULATION OF SITES The Nuclear Installations (Dangerous Occurrences) Regulations 1965...

  17. Collegiate Wind Competition Rules and Regulations | Department...

    Energy Savers [EERE]

    Rules and Regulations Collegiate Wind Competition Rules and Regulations The competition challenges interdisciplinary teams of undergraduate students from a variety of academic...

  18. Small Molecule Regulation of Alternative Splicing

    E-Print Network [OSTI]

    Anderson, Erik Steven

    2012-01-01

    digitoxin regulates alternative splicing through depletion2003. Mechanisms of alternative pre-messenger RNA splicing.digitoxin regulates alternative splicing through depletion

  19. International Electricity Regulation | Department of Energy

    Energy Savers [EERE]

    Regulation International Electricity Regulation U.S. trade in electric energy with Canada and Mexico is rising, bringing economic and reliability benefits to the United States and...

  20. Price regulation for waste hauling franchises in California: an examination of how regulators regulate pricing and the effects of competition on regulated markets

    E-Print Network [OSTI]

    Seltzer, Steven A.

    2011-01-01

    Thomadakis, Stavros. “Price Regulation Under Uncertainty in698. Bös, Dieter. Pricing and Price Regulation. Elsevier.Optimal Structure of Public Prices. ” The American Economic

  1. Negative feedback regulation of Homer 1a on norepinephrine-dependent cardiac hypertrophy

    SciTech Connect (OSTI)

    Chiarello, Carmelina; Bortoloso, Elena; Carpi, Andrea; Furlan, Sandra; Volpe, Pompeo, E-mail: pompeo.volpe@unipd.it

    2013-07-15

    Homers are scaffolding proteins that modulate diverse cell functions being able to assemble signalling complexes. In this study, the presence, sub-cellular distribution and function of Homer 1 was investigated. Homer 1a and Homer 1b/c are constitutively expressed in cardiac muscle of both mouse and rat and in HL-1 cells, a cardiac cell line. As judged by confocal immunofluorescence microscopy, Homer 1a displays sarcomeric and peri-nuclear localization. In cardiomyocytes and cultured HL-1 cells, the hypertrophic agonist norepinephrine (NE) induces ?{sub 1}-adrenergic specific Homer 1a over-expression, with a two-to-three-fold increase within 1 h, and no up-regulation of Homer 1b/c, as judged by Western blot and qPCR. In HL-1 cells, plasmid-driven over-expression of Homer 1a partially antagonizes activation of ERK phosphorylation and ANF up-regulation, two well-established, early markers of hypertrophy. At the morphometric level, NE-induced increase of cell size is likewise and partially counteracted by exogenous Homer 1a. Under the same experimental conditions, Homer 1b/c does not have any effect on ANF up-regulation nor on cell hypertrophy. Thus, Homer 1a up-regulation is associated to early stages of cardiac hypertrophy and appears to play a negative feedback regulation on molecular transducers of hypertrophy. -- Highlights: • Homer 1a is constitutively expressed in cardiac tissue. • In HL-1 cells, norepinephrine activates signaling pathways leading to hypertrophy. • Homer 1a up-regulation is an early event of norepinephrine-induced hypertrophy. • Homer 1a plays a negative feedback regulation modulating pathological hypertrophy. • Over-expression of Homer 1a per se does not induce hypertrophy.

  2. Noise-induced transitions vs. noise-induced phase transitions

    E-Print Network [OSTI]

    Toral, Raúl

    Noise-induced transitions vs. noise-induced phase transitions Raul Toral IFISC (Instituto de Física the field of noise-induced phase transitions, emphasizing the main differences with the phase-induced transitions and showing that they appear in different systems. I will show that a noise-induced transition can

  3. The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana W

    E-Print Network [OSTI]

    Pauly, Markus

    influence on its ability to protect the plant against various forms of biotic and abiotic stress. WAX INDUCER1 (WIN1) and related transcription factors have recently been shown to trigger wax production of genes associated with wax biosynthesis, suggesting that the regulation of cutin and wax production

  4. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway [Richland, WA; Skeen, Rodney S [Pendleton, OR; Hooker, Brian S [Kennewick, WA; Anderson, Daniel B [Pasco, WA

    2003-04-01

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  5. Mechanical regulation of signaling pathways in bone, William R. Thompson a,

    E-Print Network [OSTI]

    , cyclooxygenase 2; LMMS, low magnitude mechanical stimulation; LIV, low intensity vibration; CT, micro compReview Mechanical regulation of signaling pathways in bone, William R. Thompson a, , Clinton T A wide range of cell types depend on mechanically induced signals to enable appropriate physiological re

  6. SITE PLAN REVIEW REGULATIONS DURHAM, NEW HAMPSHIRE

    E-Print Network [OSTI]

    SITE PLAN REVIEW REGULATIONS of DURHAM, NEW HAMPSHIRE SECTION 1: Authority and Purpose 1 as the SITE PLAN REVIEW REGULATIONS OF DURHAM, NEW HAMPSHIRE, and supercede the Site Plan Regulations, Town of Durham, New Hampshire, Adopted December 12, 1990, as amended prior hereto, and such prior regulations

  7. OUTPUT REGULATION OF NONLINEAR NEUTRAL SYSTEMS

    E-Print Network [OSTI]

    Fridman, Emilia

    OUTPUT REGULATION OF NONLINEAR NEUTRAL SYSTEMS Emilia Fridman1 Department of Electrical Engineering, Tel-Aviv University Ramat-Aviv, Tel-Aviv 69978, Israel emilia@eng.tau.ac.il Summary. Output regulation regulation, regulator equations, center manifold 1 Introduction One of the most important problems in control

  8. The Building (First Amendment) Regulations 1965 

    E-Print Network [OSTI]

    Pannell, Charles

    1965-01-01

    STATUTORY INSTRUMENTS 1965 No. 2184 BUILDING AND BUILDINGS The Building (First Amendment) Regulations 1965

  9. Regulations and Basic Information Table of Contents

    E-Print Network [OSTI]

    Liskiewicz, Maciej

    Regulations and Basic Information Table of Contents Safe and Effective Use.) for Various Quantities of Water

  10. SB 4 Well Stimulation Treatment Regulations Text of Proposed Regulations

    E-Print Network [OSTI]

    . CHAPTER 4. DEVELOPMENT, REGULATION, AND CONSERVATION OF OIL AND GAS RESOURCES Subchapter 2. Environmental for those operations from the Division. NOTE: Authority cited: Sections 3013 and 3160, Public Resources Code. Reference: Sections 3106, 3160, and 3203 Public Resources Code. Article 2. Definitions 1761. Well

  11. Dynamics of the Oil Transition: Modeling Capacity, Costs, and Emissions

    E-Print Network [OSTI]

    Brandt, Adam R.; Farrell, Alexander E.

    2008-01-01

    entirely from steam-induced heavy oil production in Cali-from Venezuela’s extra-heavy oil reached about 0.6 Mbbl/d inliquid fuels. Heavy and extra-heavy oil are very viscous and

  12. cis-and trans-Regulation of miR163 and Target Genes Confers Natural Variation of Secondary Metabolites in Two Arabidopsis

    E-Print Network [OSTI]

    Tholl, Dorothea

    involved in secondary metabolite biosynthetic pathways that are inducible by a fungal elicitor, alamethicin and secondary metabolite profiles. We suggest that cis- and trans-regulation of miRNA and other genes providescis- and trans-Regulation of miR163 and Target Genes Confers Natural Variation of Secondary

  13. Metal specificity and the mechanism of allosteric regulation in metal-sensing metal-responsive transcriptional repressors Staphylococcus aureus CzrA and Mycobacterium tuberculosis NmtR 

    E-Print Network [OSTI]

    Pennella, Mario Antonio

    2005-08-29

    . In contrast, NmtR adopts 5- or 6-coordinate complexes with Ni(II) and Co(II), the strongest allosteric regulators of nmt O/P binding in vitro and de-repression in vivo. Zn(II), a non-inducer in vivo and poor regulator in vitro, binds NmtR with high affinity...

  14. Reciprocal regulation of LXR? activity by ASXL1 and ASXL2 in lipogenesis

    SciTech Connect (OSTI)

    Park, Ui-Hyun; Seong, Mi-ran [Department of Bioscience and Biotechnology, Institute of Bioscience, BK21 Graduate Program, Sejong University, Seoul 143-747 (Korea, Republic of)] [Department of Bioscience and Biotechnology, Institute of Bioscience, BK21 Graduate Program, Sejong University, Seoul 143-747 (Korea, Republic of); Kim, Eun-Joo; Hur, Wonhee; Kim, Sung Woo [Department of Molecular Biology, BK21 Graduate Program, Dankook University, Gyeonggi-do 448-701 (Korea, Republic of)] [Department of Molecular Biology, BK21 Graduate Program, Dankook University, Gyeonggi-do 448-701 (Korea, Republic of); Yoon, Seung Kew [The Catholic University Liver Research Center and WHO Collaborating Center of Viral Hepatitis, The Catholic University, College of Medicine, Seoul 137-701 (Korea, Republic of)] [The Catholic University Liver Research Center and WHO Collaborating Center of Viral Hepatitis, The Catholic University, College of Medicine, Seoul 137-701 (Korea, Republic of); Um, Soo-Jong, E-mail: umsj@sejong.ac.kr [Department of Bioscience and Biotechnology, Institute of Bioscience, BK21 Graduate Program, Sejong University, Seoul 143-747 (Korea, Republic of)] [Department of Bioscience and Biotechnology, Institute of Bioscience, BK21 Graduate Program, Sejong University, Seoul 143-747 (Korea, Republic of)

    2014-01-10

    Highlights: •ASXL1 and ASXL2 directly interact with ligand-bound LXR?. •Ligand-induced LXR? activity is repressed by ASXL1 and activated by ASXL2. •ASXL1 and ASXL2 bind to the LXRE of the LXR? target promoter. •ASXL1 and ASXL2 reciprocally regulate lipogenesis in liver cells. -- Abstract: Liver X receptor alpha (LXR?), a member of the nuclear receptor superfamily, plays a pivotal role in hepatic cholesterol and lipid metabolism, regulating the expression of genes associated with hepatic lipogenesis. The additional sex comb-like (ASXL) family was postulated to regulate chromatin function. Here, we investigate the roles of ASXL1 and ASXL2 in regulating LXR? activity. We found that ASXL1 suppressed ligand-induced LXR? transcriptional activity, whereas ASXL2 increased LXR? activity through direct interaction in the presence of the ligand. Chromatin immunoprecipitation (ChIP) assays showed ligand-dependent recruitment of ASXLs to ABCA1 promoters, like LXR?. Knockdown studies indicated that ASXL1 inhibits, while ASXL2 increases, lipid accumulation in H4IIE cells, similar to their roles in transcriptional regulation. We also found that ASXL1 expression increases under fasting conditions, and decreases in insulin-treated H4IIE cells and the livers of high-fat diet-fed mice. Overall, these results support the reciprocal role of the ASXL family in lipid homeostasis through the opposite regulation of LXR?.

  15. Administrative Regulation 9:1 Page 1 of 6 Administrative Regulation 9:1

    E-Print Network [OSTI]

    MacAdam, Keith

    Administrative Regulation 9:1 Page 1 of 6 Administrative Regulation 9:1 Responsible Office: Vice/3/1985 Regulations Governing Time, Place, and Manner of Meetings, Demonstrations, and Other Assemblies Regulation of their exercise and the rights of others in the effective use of University facilities, the following regulations

  16. California Energy Commission ADOPTED REGULATIONS

    E-Print Network [OSTI]

    and Energy Division 2. State Energy Resources Conservation and Development Commission Chapter 4. Energy Resources Code Section 25402.10, for disclosing energy use data and ENERGY STAR® Energy Performance ScoresCalifornia Energy Commission ADOPTED REGULATIONS FEBRUARY 2013 CEC-400-2010-004-CMF

  17. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Purchase and Sale of Real Property 6C7-7.202 SUMMARY OF REGULATION REPEAL: This regulation is not necessary and does REGULATION REPEAL: Regulations Administrator COMMENTS CONCERNING THE REGULATION REPEAL SHOULD BE SUBMITTED

  18. NOTICE OF REGULATION REPEAL Date: June 9, 2014

    E-Print Network [OSTI]

    Pattanaik, Sumanta N.

    NOTICE OF REGULATION REPEAL Date: June 9, 2014 REGULATION TITLE: REGULATION NO.: Tenure UCF-3.011 SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal because the material contained in this regulation has been moved to regulation UCF-3.015. AUTHORITY: BOG Regulations 1.001 NAME OF PERSON WHO

  19. NOTICE OF REGULATION REPEAL Date: May 24, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: May 24, 2010 REGULATION TITLE: REGULATION NO.: Conflict of Interest 6C7-3.008 SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal, because the material contained in this regulation has been moved to regulation UCF-3.018 AUTHORITY: BOG Regulation 1

  20. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  1. TL1A induces the expression of TGF-b-inducible gene h3 (big-h3) through PKC, PI3K, and ERK in THP-1 cells

    E-Print Network [OSTI]

    Lee, Won-Ha

    TL1A induces the expression of TGF-b-inducible gene h3 (big-h3) through PKC, PI3K, and ERK in THP-1 of protein kinase C (PKC), extracellular signal-regulated kinase (ERK), phosphoinositide kinase-3 (PI3K blocked the phos- phorylation of AKT without affecting ERK phosphorylation. On the other hand, suppression

  2. Involvement of caspase-12-dependent apoptotic pathway in ionic radiocontrast urografin-induced renal tubular cell injury

    SciTech Connect (OSTI)

    Wu, Cheng Tien [Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China)] [Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China); Weng, Te I. [Department of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan (China)] [Department of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan (China); Chen, Li Ping [Department of Dentistry, Chang Gang Memorial Hospital, Chang Gang University, Taoyuan, Taiwan (China)] [Department of Dentistry, Chang Gang Memorial Hospital, Chang Gang University, Taoyuan, Taiwan (China); Chiang, Chih Kang [Department of Integrated Diagnostics and Therapeutics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan (China) [Department of Integrated Diagnostics and Therapeutics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan (China); Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan (China); Liu, Shing Hwa, E-mail: shinghwaliu@ntu.edu.tw [Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China); Department of Urology, National Taiwan University Hospital, Taipei, Taiwan (China)

    2013-01-01

    Contrast medium (CM) induces a direct toxic effect on renal tubular cells. This toxic effect subjects in the disorder of CM-induced nephropathy. Our previous work has demonstrated that CM shows to activate the endoplasmic reticulum (ER)-related adaptive unfolding protein response (UPR) activators. Glucose-regulated protein 78 (GRP78)/eukaryotic initiation factor 2? (eIF2?)-related pathways play a protective role during the urografin (an ionic CM)-induced renal tubular injury. However, the involvement of ER stress-related apoptotic signals in the urografin-induced renal tubular cell injury remains unclear. Here, we examined by the in vivo and in vitro experiments to explore whether ER stress-regulated pro-apoptotic activators participate in urografin-induced renal injury. Urografin induced renal tubular dilation, tubular cells detachment, and necrosis in the kidneys of rats. The tubular apoptosis, ER stress-related pro-apoptotic transcriptional factors, and kidney injury marker-1 (kim-1) were also conspicuously up-regulated in urografin-treated rats. Furthermore, treatment of normal rat kidney (NRK)-52E tubular cells with urografin augmented the expressions of activating transcription factor-6 (ATF-6), C/EBP homologous protein (CHOP), Bax, caspase-12, JNK, and inositol-requiring enzyme (IRE) 1 signals. Urografin-induced renal tubular cell apoptosis was not reversed by the inhibitors of ATF-6, JNK signals or CHOP siRNA transfection, but it could be partially reversed by the inhibitor of caspase-12. Taken together, the present results and our previous findings suggest that exposure of CM/urografin activates the ER stress-regulated survival- and apoptosis-related signaling pathways in renal tubular cells. Caspase-12-dependent apoptotic pathway may be partially involved in the urografin-induced nephropathy. -- Highlights: ? Ionic contrast medium-urografin induces renal tubular cell apoptosis. ? Urografin induces the ER stress-regulated survival and apoptosis signals. ? Caspase-12 signaling participates in urografin-induced renal cell apoptosis.

  3. Genomic neighbourhood and the regulation of gene expression Genomic neighbourhood and transcriptional regulation

    E-Print Network [OSTI]

    Babu, M. Madan

    Genomic neighbourhood and the regulation of gene expression Genomic neighbourhood and transcriptional regulation Subhajyoti De and M. Madan Babu MRC Laboratory of Molecular Biology, Hills Road..................................................................................................................................................................................1 2. Genomic neighbourhood and its influence on gene regulation

  4. Naturalistic emotion regulation: The measurement and social consequences of spontaneous emotion regulation during marital conflict.

    E-Print Network [OSTI]

    Bloch, Lian Michal

    2011-01-01

    On the Nature of Emotion Regulation. Child Development, 75(2011). Reconceptualizing emotion regulation. Emotion Review.L.L. (2007). Emotion regulation and aging. In J.J. Gross (

  5. Implementation Regulations 3TU MSc Sustainable Energy Technology Delft, 2014-2015 THE IMPLEMENTATION REGULATIONS

    E-Print Network [OSTI]

    van Vliet, Lucas J.

    Implementation Regulations 3TU MSc Sustainable Energy Technology ­ Delft, 2014-2015 THE IMPLEMENTATION REGULATIONS 2014 - 2015 3TU MASTER'S DEGREE PROGRAMME Sustainable Energy Technology - Delft (SET) DELFT UNIVERSITY OF TECHNOLOGY #12;Implementation Regulations MSc Sustainable Energy Technology SET

  6. Cell, Vol. 90, 4353, July 11, 1997, Copyright 1997 by Cell Press A Small, Stable RNA Induced by Oxidative

    E-Print Network [OSTI]

    Mekalanos, John

    suggest that the oxyS RNA acts as a regulator thatintegrates adaptation to hydro- A Small RNA InducedCell, Vol. 90, 43­53, July 11, 1997, Copyright ©1997 by Cell Press A Small, Stable RNA Induced-Fischer, While unique functions have been discovered for a subset of the small RNA population, the roles of other

  7. Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004

    Office of Energy Efficiency and Renewable Energy (EERE)

    Handbook providing practical information to help regulators decide if they want to use output-based regulations and explains how to develop an output-based emission standard

  8. Voltage Regulation PHYS 309 Name

    E-Print Network [OSTI]

    Herman, Rhett

    an regulated output of 5. It will also put out up to 1.5 yielding a power of 7.5. Obviously there are some LED circuits is to be used as a switch. Use 6 identical LEDs in this circuit. Use a multimeter to check that the LEDs all activate at the same voltage. Record the value of the LED voltage and your Zener

  9. Frequency regulator for synchronous generators

    DOE Patents [OSTI]

    Karlicek, R.F.

    1982-08-10

    The present invention is directed to a novel frequency regulator which controls a generator output frequency for variations in both the input power to the generator and the power supplied to an uncontrolled external load. The present invention further includes over current and current balance protection devices which are relatively inexpensive to manufacture, which may be encapsulated to provide protection from the operating environment and which respond more quickly than previously known electromechanical devices. 11 figs.

  10. Frequency regulator for synchronous generators

    DOE Patents [OSTI]

    Karlicek, Robert F. (1920 Camino Centroloma, Fullerton, CA 92633)

    1982-01-01

    The present invention is directed to a novel frequency regulator which controls a generator output frequency for variations in both the input power to the generator and the power supplied to an uncontrolled external load. The present invention further includes over current and current balance protection devices which are relatively inexpensive to manufacture, which may be encapsulated to provide protection from the operating environment and which respond more quickly than previously known electromechanical devices.

  11. La enseñanza teatral en Venezuela

    E-Print Network [OSTI]

    Rodrí guez, Orlando

    1988-04-01

    cursos y talleres breves sobre otras áreas. Una sola escuela, la Nacional de Artes Escénicas César Rengifo tiene, además de la preparación actoral, dos especialidades fundamentales para el teatro venezolano: formación de diseñadores... extremas del país. Un solo instituto, la Universidad Central de Vene zuela, mediante su Escuela de Artes, en la Mención Artes Escénicas, forma a nivel superior críticos, historiadores y teóricos del teatro. En otras palabras, la única formación...

  12. NRC - regulator of nuclear safety

    SciTech Connect (OSTI)

    1997-05-01

    The U.S. Nuclear Regulatory Commission (NRC) was formed in 1975 to regulate the various commercial and institutional uses of nuclear energy, including nuclear power plants. The agency succeeded the Atomic Energy Commission, which previously had responsibility for both developing and regulating nuclear activities. Federal research and development work for all energy sources, as well as nuclear weapons production, is now conducted by the U.S. Department of Energy. Under its responsibility to protect public health and safety, the NRC has three principal regulatory functions: (1) establish standards and regulations, (2) issue licenses for nuclear facilities and users of nuclear materials, and (3) inspect facilities and users of nuclear materials to ensure compliance with the requirements. These regulatory functions relate to both nuclear power plants and to other uses of nuclear materials - like nuclear medicine programs at hospitals, academic activities at educational institutions, research work, and such industrial applications as gauges and testing equipment. The NRC places a high priority on keeping the public informed of its work. The agency recognizes the interest of citizens in what it does through such activities as maintaining public document rooms across the country and holding public hearings, public meetings in local areas, and discussions with individuals and organizations.

  13. Petroleum: The Petroleum (Production) (Amendment) Regulations, 1957 

    E-Print Network [OSTI]

    Her Majesty's Stationary Office

    1957-01-01

    These Regulations amend the Petroleum (Production) Regulations, 1935, which set out the requirements for applications for, and the model clauses to be incorporated in, prospecting and mining licences issued under the ...

  14. University of Massachusetts Fair Information Practices Regulations

    E-Print Network [OSTI]

    Massachusetts at Lowell, University of

    Information Under the University's Fair Information Practices Regulations, I have the right to requestUniversity of Massachusetts Fair Information Practices Regulations Directive Regarding Directory that certain personal information which is regarded by the University as "Directory Information

  15. The Building Standard (Scotland) Amendment Regulations 1964 

    E-Print Network [OSTI]

    Noble, Michael

    1964-01-01

    STATUTORY INSTRUMENTS 1964 No. 802 (S. 50) BUILDING AND BUILDINGS The Building Standards (Scotland) Amendment Regulations 1964

  16. Regulations for beneficial use changing nationwide

    SciTech Connect (OSTI)

    Goss, D.

    2008-07-01

    Regulations on the use of coal combustion products (CCPs) that are being considered by various US states are reviewed.

  17. Final Report for Regulation of Embryonic Development in Higher Plants

    SciTech Connect (OSTI)

    Harada, John J.

    2013-10-22

    The overall goal of the project was to define the cellular processes that underlie embryo development in plants at a mechanistic level. Our studies focused on a critical transcriptional regulator, Arabidopsis LEAFY COTYLEDON (LEC1), that is necessary and sufficient to induce processes required for embryo development. Because LEC1 regulates lipid accumulation during the maturation phase of embryo development, information about LEC1 may be useful in designing approaches to enhance biofuel production in plants. During the tenure of this project, we determined the molecular mechanisms by which LEC1 acts as a transcription factor in embryos. We also identified genes directly regulated by LEC1 and showed that many of these genes are involved in maturation processes. This information has been useful in dissecting the gene regulatory networks controlling embryo development. Finally, LEC1 is a novel isoform of a transcription factor that is conserved among eukaryotes, and LEC1 is active primarily in seeds. Therefore, we determined that the LEC1-type transcription factors first appeared in lycophytes during land plant evolution. Together, this study provides basic information that has implications for biofuel production.

  18. Physiology and Regulation of Calcium Channels in Stomatal Guard Cells

    SciTech Connect (OSTI)

    Schroeder, Julian I.

    2007-05-02

    Stomatal pores in the epidermis of leaves regulate the diffusion of CO2 into leaves for photosynthetic carbon fixation and control water loss of plants during drought periods. Guard cells sense CO2, water status, light and other environmental conditions to regulate stomatal apertures for optimization of CO2 intake and plant growth under drought stress. The cytosolic second messenger calcium contributes to stomatal movements by transducing signals and regulating ion channels in guard cells. Studies suggest that both plasma membrane Ca2+ influx channels and vacuolar/organellar Ca2+ release channels contribute to ABA-induced Ca2+ elevations in guard cells. Recent research in the P.I.'s laboratory has led to identification of a novel major cation-selective Ca2+-permeable influx channel (Ica) in the plasma membrane of Arabidopsis guard cells. These advances will allow detailed characterization of Ica plasma membrane Ca2+ influx channels in guard cells. The long term goal of this research project is to gain a first detailed characterization of these novel plasma membrane Ca2+-permeable channel currents in Arabidopsis guard cells. The proposed research will investigate the hypothesis that Ica represents an important Ca2+ influx pathway for ABA and CO2 signal transduction in Arabidopsis guard cells. These studies will lead to elucidation of key signal transduction mechanisms by which plants balance CO2 influx into leaves and transpirational water loss and may contribute to future strategies for manipulating gas exchange for improved growth of crop plants and for biomass production.

  19. Regulation of Hydraulic Fracturing in California

    E-Print Network [OSTI]

    Kammen, Daniel M.

    APRIL 2013 Regulation of Hydraulic Fracturing in California: A WAsteWAteR And WAteR QuAlity Pe | Regulation of Hydraulic Fracturing in California Wheeler Institute for Water Law & Policy Center for Law #12;Regulation of Hydraulic Fracturing in California | 3Berkeley law | wheeler InstItute for water law

  20. A Computational Model for Adaptive Emotion Regulation

    E-Print Network [OSTI]

    Treur, Jan

    A Computational Model for Adaptive Emotion Regulation Tibor Bosse, Matthijs Pontier, and Jan Treur} Abstract. Emotion regulation describes how a subject can use certain strategies to affect emotion response levels. Usually, models for emotion regulation as- sume mechanisms based on feedback loops that indicate

  1. Army Regulation 2555 Information Management: Records

    E-Print Network [OSTI]

    US Army Corps of Engineers

    Army Regulation 25­55 Information Management: Records Management The Department of the Army Freedom Incorporates the Interim Change No. I01, which expires 30 June 1996 into the baseline regulation dated 10 of Information Act Program *Army Regulation 25­55 Effective 1 December 1997 History. This revision was originally

  2. 2013-2014 Case Summaries Regulation 1

    E-Print Network [OSTI]

    Royer, Dana

    2013-2014 Case Summaries Regulation 1 Disturbance of the Peace ­ the infringement upon the right that repeated violation of this regulation could result in administrative reassignment to another residential violated Section II, Regulations 1 and 14 of the Code of Non-Academic Conduct. The board found the group

  3. Optically Induced Transparency

    E-Print Network [OSTI]

    Zheng, Yuanlin; Shen, Zhenhua; Cao, Jianjun; Chen, Xianfeng; Liang, Xiaogan; Wan, Wenjie

    2015-01-01

    Light-matter-light interactions serve as the backbone technology of all-optical information processing for both on-chip and long-haul communication purposes. The representative example of electromagnetically induced transparency has its unique ability of optically controlling transparency windows with relative low light in atomic systems, though its practical applications are limited due to rigid experimental requirements. Here we demonstrate a new form of optically induced transparency in a micro-cavity by introducing four-wave mixing gain in order to couple nonlinearly two separated resonances of the micro-cavity in ambient environment. A signature Fano-like resonance is also observed owing to the nonlinear interference of two coupled resonances. Moreover, we show that the unidirectional gain of four-wave mixing can lead to non-reciprocal transmission at the transparency windows. Optically induced transparency may offer a unique platform for a compact, integrated solution to all-optical processing and quant...

  4. NOTICE OF REGULATION REPEAL Date: May 5, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: May 5, 2009 REGULATION TITLE: REGULATION NO.: Student Rights and Responsibilities 6C7-5.003 SUMMARY OF REGULATION REPEAL: This regulation is being repealed. New regulations OF PERSON WHO INITIATED REGULATION REPEAL: Patricia MacKown, Director, Office of Student Rights

  5. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Rental Rates 6C7-7.219 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal because the subject matter of the regulation is not needed and is out of date. Florida Board of Governors Regulation 17.001 requires

  6. NOTICE OF REGULATION REPEAL Date: June 9, 2014

    E-Print Network [OSTI]

    Pattanaik, Sumanta N.

    NOTICE OF REGULATION REPEAL Date: June 9, 2014 REGULATION TITLE: REGULATION NO.: Promotion of Tenured and Tenure-earning faculty UCF-3.017 SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal because the material contained in this regulation has been moved to regulation UCF-3

  7. Homeobox A7 stimulates breast cancer cell proliferation by up-regulating estrogen receptor-alpha

    SciTech Connect (OSTI)

    Zhang, Yu; Department of Obstetrics and Gynaecology, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4 ; Cheng, Jung-Chien; Huang, He-Feng; Leung, Peter C.K.

    2013-11-01

    Highlights: •HOXA7 regulates MCF7 cell proliferation. •HOXA7 up-regulates ER? expression. •HOXA7 mediates estrogen-induced MCF7 cell proliferation. -- Abstract: Breast cancer is the most common hormone-dependent malignancy in women. Homeobox (HOX) transcription factors regulate many cellular functions, including cell migration, proliferation and differentiation. The aberrant expression of HOX genes has been reported to be associated with human reproductive cancers. Estradiol (E2) and its nuclear receptors, estrogen receptor (ER)-alpha and ER-beta, are known to play critical roles in the regulation of breast cancer cell growth. However, an understanding of the potential relationship between HOXA7 and ER in breast cancer cells is limited. In this study, our results demonstrate that knockdown of HOXA7 in MCF7 cells significantly decreased cell proliferation and ER? expression. In addition, HOXA7 knockdown attenuated E2-induced cell proliferation as well as progesterone receptor (PR) expression. The stimulatory effects of E2 on cell proliferation and PR expression were abolished by co-treatment with ICI 182780, a selective ER? antagonist. In contrast, overexpression of HOXA7 significantly stimulated cell proliferation and ER? expression. Moreover, E2-induced cell proliferation, as well as PR expression, was enhanced by the overexpression of HOXA7. Neither knockdown nor overexpression of HOXA7 affected the ER-beta levels. Our results demonstrate a novel mechanistic role for HOXA7 in modulating breast cancer cell proliferation via regulation of ER? expression. This finding contributes to our understanding of the role HOXA7 plays in regulating the proliferation of ER-positive cancer cells.

  8. Neutron-induced nucleosynthesis

    E-Print Network [OSTI]

    H. Oberhummer; H. Herndl; T. Rauscher; H. Beer

    1996-08-20

    Neutron--induced nucleosynthesis plays an important role in astrophysical scenarios like in primordial nucleosynthesis in the early universe, in the s--process occurring in Red Giants, and in the $\\alpha$--rich freeze--out and r--process taking place in supernovae of type II. A review of the three important aspects of neutron--induced nucleosynthesis is given: astrophysical background, experimental methods and theoretical models for determining reaction cross sections and reaction rates at thermonuclear energies. Three specific examples of neutron capture at thermal and thermonuclear energies are discussed in some detail.

  9. DEPARTMENT OF PESTICIDE REGULATION http://www.clemson.edu/public/regulatory/pesticide_regulation/

    E-Print Network [OSTI]

    Stuart, Steven J.

    DEPARTMENT OF PESTICIDE REGULATION http://www.clemson.edu/public/regulatory/pesticide_regulation/ The Department of Pesticide Regulation (DPR) is the South Carolina regulatory and investigative agency Westinghouse Road Pendleton, SC 29670 TEL: 864-646-2151 FAX: 864-646-2179 Department of Pesticide Regulation

  10. Assessing Emotion Regulation in Social Anxiety Disorder: The Emotion Regulation Interview

    E-Print Network [OSTI]

    Gross, James J.

    Assessing Emotion Regulation in Social Anxiety Disorder: The Emotion Regulation Interview Kelly H dysregulation in SAD has not been well characterized. In the present study, the Emotion Regulation Interview (ERI) was developed to quantify the frequency and self-efficacy of five emotion regulation strategies

  11. Emotion Regulation as Situated Conceptualizations 1 RUNNING HEAD: EMOTION REGULATION AS SITUATED CONCEPTUALIZATIONS

    E-Print Network [OSTI]

    Barsalou, Lawrence W.

    Emotion Regulation as Situated Conceptualizations 1 RUNNING HEAD: EMOTION REGULATION AS SITUATED CONCEPTUALIZATIONS TITLE: A psychological construction account of emotion regulation and dysregulation: The role Boston, MA 02115 Phone: 617-373-2044 Fax: 617-373-8714 Email: l.barrett@neu.edu #12;Emotion Regulation

  12. IMPLICIT EVALUATION OF EMOTION REGULATION 1 Running Head: IMPLICIT EVALUATION OF EMOTION REGULATION

    E-Print Network [OSTI]

    Gross, James J.

    IMPLICIT EVALUATION OF EMOTION REGULATION 1 Running Head: IMPLICIT EVALUATION OF EMOTION REGULATION How to Bite Your Tongue without Blowing Your Top: Implicit Evaluation of Emotion Regulation Predicts EVALUATION OF EMOTION REGULATION 2 Abstract People frequently have to control their emotions to function

  13. Administrative Regulation 6:4 Page 1 of 6 Administrative Regulation 6:4

    E-Print Network [OSTI]

    MacAdam, Keith

    Administrative Regulation 6:4 Page 1 of 6 Administrative Regulation 6:4 Responsible Office: EVPFA. Introduction This regulation establishes the University's policies and procedures for using, serving, local ordinances, and University regulations. All members of the University community shall abide

  14. Regulation and the High Cost of Housing in California

    E-Print Network [OSTI]

    Quigley, John M.; Raphael, Steven

    2006-01-01

    C. Tsuriel. “Land Use Regulations and New Construc- tion. ”local government land use regulation. Cam- “The Effects offrequently the target of regulation. REFERENCES Glickfeld,

  15. Land use regulation and intraregional population-employment interaction

    E-Print Network [OSTI]

    Kim, JH; Hewings, GJD

    2013-01-01

    Levine J (2006) Zoned out: Regulation, markets, and choicesprices, externalities, and regulation in U.S. metropolitanCT (2000) Land use regulation and new construction. Regional

  16. Collegiate Wind Competition 2014 Rules and Regulations | Department...

    Office of Environmental Management (EM)

    Collegiate Wind Competition 2014 Rules and Regulations Collegiate Wind Competition 2014 Rules and Regulations This document outlines the rules and regulations for the inaugural...

  17. Chapter 9 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    of the Wyoming Public Service Commission Regulations: General Forms Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  18. Improving Regulation Through Incremental Adjustment

    E-Print Network [OSTI]

    Glicksman, Robert L.; Shapiro, Sidney A.

    2004-03-01

    if it oc curred"). 46. Schuck, supra note 44, at 283 ("By reducing the hardships and the sense of injustice suf· fered by those to whom a rule applies, exceptions diminish the pressure to challenge the rule it self."). 47. WAIT Radio v. FCC, 418 F.2d 1153... C.P.R. § 125.85(a)(2». . 1194 b. Evaluation KANSAS LAW REVIEW [Vol. 52 A regulated entity is entitled to a harm-based adjustment if it can establish that the adjustment would not lead to decreased protection for people or the environment. When...

  19. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, S.Y.

    1989-08-08

    An improvement in a pump is described including a shrouded inducer, the improvement comprising first and second sealing means which cooperate with a first vortex cell and a series of secondary vortex cells to remove any tangential velocity components from the recirculation flow. 3 figs.

  20. Genetic analysis of the stationary-phase induced and [sigma]S-independent promoter of the mcb operon in Escherichia coli 

    E-Print Network [OSTI]

    Mao, Weimin

    1997-01-01

    , or enter stationary phase in rich medium. This induction is independent of all regulatory pathways currently known to be involved in regulating expression of other stationary-phase induced genes. To understand the molecular mechanisms controlling...

  1. Regulation of the PDK4 Isozyme by the Rb-E2F1 Complex*S Received for publication,March 27, 2008, and in revised form, July 24, 2008 Published, JBC Papers in Press,July 30, 2008, DOI 10.1074/jbc.M802418200

    E-Print Network [OSTI]

    Regulation of the PDK4 Isozyme by the Rb-E2F1 Complex*S Received for publication,March 27, 2008-induced diabetes. Here, we dem- onstrate that E2F1 directly regulates the gene encoding PDK4 (pyruvate sen- sitivity to insulin stimulation. Consistently, enforced E2F1 expression up-regulates PDK4 levels

  2. Volume regulation by human lymphocytes. Role of calcium

    SciTech Connect (OSTI)

    Grinstein, S.; Dupre, A.; Rothstein, A.

    1982-05-01

    Human peripheral blood lymphocytes regulate their volumes in hypotonic solutions. In hypotonic media in which Na+ is the predominant cation, an initial swelling phase is followed by a regulatory volume decrease (RVD) associated with a net loss of cellular K+. In media in which K+ is the predominant cation, the rapid initial swelling is followed by a slower second swelling phase. /sup 86/Rb+ fluxes increased during RVD and returned to normal when the original volume was approximately regained. Effects similar to those induced by hypotonic stress could also be produced by raising the intracellular Ca++ level. In isotonic, Ca++-containing media cells were found to shrink upon addition of the Ca++ ionophore A23187 in K+-free media, but to swell in K+-rich media. Exposure to Ca++ plus A23187 also increased /sup 86/Rb+ fluxes. Quinine (75 microM), an inhibitor of the Ca++-activated K+ pathway in other systems blocked RVD, the associated K+ loss, and the increase in /sup 86/Rb+ efflux. Quinine also inhibited the volume changes and the increased /sup 86/Rb fluxes induced by Ca++ plus ionophore. The calmodulin inhibitors trifluoperazine, pimozide and chlorpromazine blocked RVD as well as Ca++ plus A23187-induced volume changes. Trifluoperazine also prevented the increase in /sup 86/Rb+ fluxes and K+ loss induced by hypotonicity. Chlorpromazine sulfoxide, a relatively ineffective calmodulin antagonist, was considerably less potent as an inhibitor of RVD than chlorpromazine. It is suggested than an elevation in cytoplasmic (Ca++), triggered by cell swelling, increases the plasma membrane permeability to K+, the ensuing increased efflux of K+, associated anions, and osmotically obliged water, leading to cell shrinking (RVD).

  3. Forms Supplement page -1 Export Administration Regulations January 2001

    E-Print Network [OSTI]

    Bernstein, Daniel

    Forms Supplement page - 1 Export Administration Regulations January 2001 #12;Forms Supplement page - 2 Export Administration Regulations January 2001 #12;Forms Supplement page - 3 Export Administration Regulations January 2001 #12;Forms Supplement page - 4 Export Administration Regulations January 2001 #12

  4. Geometrically induced magnetic catalysis and critical dimensions

    E-Print Network [OSTI]

    Antonino Flachi; Kenji Fukushima; Vincenzo Vitagliano

    2015-04-27

    We discuss the combined effect of magnetic fields and geometry in interacting fermionic systems. At leading order in the heat-kernel expansion, the infrared singularity (that in flat space leads to the magnetic catalysis) is regulated by the chiral gap effect, and the catalysis is deactivated by the effect of the scalar curvature. We discover that an infrared singularity is found in higher-order terms that mix the magnetic field with curvature, and these lead to a novel form of geometrically induced magnetic catalysis. The dynamical mass squared is then modified not only due to the chiral gap effect by an amount proportional to the curvature, but also by a magnetic shift $\\propto (4-D)eB$, where $D$ represents the number of space-time dimensions. We argue that $D=4$ is a critical dimension across which the behavior of the magnetic shift changes qualitatively.

  5. The Arabidopsis blue light receptor cryptochrome 2 is a nuclear protein regulated by a blue light-dependent post-

    E-Print Network [OSTI]

    Lin, Chentao

    The Arabidopsis blue light receptor cryptochrome 2 is a nuclear protein regulated by a blue light Charles E. Young Dr. South, Los Angeles, CA 90095-1606, USA Summary Cryptochrome 2 is a ¯avin-type blue light receptor mediating ¯oral induction in response to photoperiod and a blue light-induced hypocotyl

  6. Comparing Emissions Benefits from Regulating Heavy Vehicle Idling...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    from Regulating Heavy Vehicle Idling Comparing Emissions Benefits from Regulating Heavy Vehicle Idling 2005 Diesel Engine Emissions Reduction (DEER) Conference...

  7. Laser-Induced Breakdown Spectroscopy

    E-Print Network [OSTI]

    Nizkorodov, Sergey

    LIBS-1 Laser-Induced Breakdown Spectroscopy LIBS ANALYSIS OF METAL SURFACES Last updated: June 17, 2014 #12;LIBS-2 Laser­Induced Breakdown Spectroscopy (LIBS) LIBS ANALYSIS OF METAL SURFACES of species at a distance or in hard­to­reach or hazardous environments. Laser­Induced Breakdown Spectroscopy

  8. Electromagnetically Induced Entanglement

    E-Print Network [OSTI]

    Xihua Yang; Min Xiao

    2015-05-18

    We present a novel quantum phenomenon named electromagnetically induced entanglement in the conventional Lambda-type three-level atomic system driven by a strong pump field and a relatively weak probe field. Nearly perfect entanglement between the pump and probe fields can be achieved with a low coherence decay rate between the two lower levels, high pump-field intensity, and large optical depth of the atomic ensemble. The physical origin is quantum coherence between the lower doublet produced by the pump and probe fields, similar to the well-known electromagnetically induced transparency. This method would greatly facilitate the generation of nondegenerate narrow-band continuous-variable entanglement between bright light beams by using only coherent laser fields, and may find potential and broad applications in realistic quantum information processing.

  9. Shear-Induced Chaos

    E-Print Network [OSTI]

    Kevin K. Lin; Lai-Sang Young

    2007-05-23

    Guided by a geometric understanding developed in earlier works of Wang and Young, we carry out some numerical studies of shear-induced chaos. The settings considered include periodic kicking of limit cycles, random kicks at Poisson times, and continuous-time driving by white noise. The forcing of a quasi-periodic model describing two coupled oscillators is also investigated. In all cases, positive Lyapunov exponents are found in suitable parameter ranges when the forcing is suitably directed.

  10. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Certification of Compliance - Leases 6C7-7.216 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal because the subject matter of the regulation is not needed and is out of date. AUTHORITY: BOG Resolution dated January

  11. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Code Compliance in Lease Space 6C7-7.207 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.203. AUTHORITY: BOG

  12. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Approval of Real Property Leases 6C7-7.205 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.203. AUTHORITY: BOG

  13. NOTICE OF REGULATION REPEAL Date: July 1, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 1, 2010 REGULATION TITLE: REGULATION NO.: Fees for Testing, Certification, Inspection and UCF-8.004 Services SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal because the material contained in this regulation has been updated and adopted as rule 6C7

  14. NOTICE OF REGULATION REPEAL Date: July 9, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 9, 2009 REGULATION TITLE: REGULATION NO.: Grievance Procedures for Non-unit Faculty and UCF-3.0132 A&P Staff Members SUMMARY OF REGULATION REPEAL: This regulation is being repealed. New regulations are being adopted that will cover the subject matter. AUTHORITY

  15. NOTICE OF REGULATION REPEAL Date: July 1, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 1, 2010 REGULATION TITLE: REGULATION NO.: Florida Solar Energy Center Operations UCF-8.001 SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal, because the material contained in this regulation has been updated and adopted as rule 6C7-8.006, F

  16. NOTICE OF REGULATION REPEAL Date: February 26, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: February 26, 2009 REGULATION TITLE: REGULATION NO.: Space Allocation 6C7-7.218 SUMMARY OF REGULATION REPEAL: This regulation is being repealed. The subject matter of regulation 6C7-7.218 is addressed by the Board of Governors. AUTHORITY: BOG Resolution dated January 7, 2003

  17. NOTICE OF REGULATION REPEAL Date: July 31, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 31, 2009 REGULATION TITLE: REGULATION NO.: Exception to Competitive Bidding for Leased Space 6C7-7.220 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7

  18. NOTICE OF REGULATION REPEAL Date: July 1, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 1, 2010 REGULATION TITLE: REGULATION NO.: Solar Thermal and Photovoltaic System UCF-8.005 Standards and Certification SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal because the material contained in this regulation has been updated and adopted as rule

  19. NOTICE OF REGULATION REPEAL Date: July 31, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 31, 2009 REGULATION TITLE: REGULATION NO.: Evaluation of Lease Proposals 6C7-7.212 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.209. AUTHORITY: BOG

  20. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Standard Lease Agreement Form 6C7-7.206 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.203. AUTHORITY: BOG

  1. NOTICE OF REGULATION REPEAL Date: May 8, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: May 8, 2009 REGULATION TITLE: REGULATION NO.: Confidential Information Policy for Faculty 6C73.030 SUMMARY OF REGULATION REPEAL: This regulation is being repealed NAME OF PERSON WHO INITIATED REGULATION REPEAL: Mark Roberts, Director, Human Resources COMMENTS

  2. NOTICE OF REGULATION REPEAL Date: July 31, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: July 31, 2009 REGULATION TITLE: REGULATION NO.: Proposals to Lease 6C7-7.211 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.209. AUTHORITY: BOG Resolution dated

  3. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Space Measurement 6C7-7.217 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.203. AUTHORITY: BOG Resolution dated

  4. NOTICE OF REGULATION REPEAL Date: August 6, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    NOTICE OF REGULATION REPEAL Date: August 6, 2009 REGULATION TITLE: REGULATION NO.: Definitions 6C7-7.204 SUMMARY OF REGULATION REPEAL: This regulation is noticed for repeal due to the inclusion of the relevant information in the proposed amendment to regulation UCF-7.203. AUTHORITY: BOG Resolution dated January 7, 2003

  5. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  6. Air Quality Regulations (Pennsylvania) | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    as pollution control devices, boilers, fuels and paints at 3,650 facilities that produce air pollution in Pennsylvania. The program administers the rules and regulations of the...

  7. Massachusetts Endangered Species Act Regulations (Massachusetts)

    Broader source: Energy.gov [DOE]

    The regulations that accompany the Massachusetts Endangered Species Act list three categories of animals and plants in need of protection: endangered, threatened, and species of special concern....

  8. Exporting licensing regulations affecting US geothermal firms

    SciTech Connect (OSTI)

    Not Available

    1988-08-01

    This document presents a brief introduction and overview of the Department of Commerce's Export Administration Regulations which might affect potential US geothermal goods exporters. It is intended to make US geothermal firms officials aware of the existence of such regulations and to provide them with references, contacts and phone numbers where they can obtain specific and detailed information and assistance. It must be stressed however, that the ultimate responsibility for complying with the above mentioned regulations lies with the exporter who must consult the complete version of the regulations.

  9. Securities Regulation in a Virtual World

    E-Print Network [OSTI]

    Thompson, Shannon L.

    2009-01-01

    Regulation in a Virtual World Shannon L. Thompson* I.A. What Is a Virtual World? . B.The Virtual World of Second Life .. 1. The

  10. Council on Environmental Quality - Regulations for Implementing...

    Open Energy Info (EERE)

    Council on Environmental Quality - Regulations for Implementing the Procedural Provisions of the NEPA Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal...

  11. Bank Regulation and Mortgage Market Reform

    E-Print Network [OSTI]

    Jaffee, Dwight M.

    2011-01-01

    Regulation and Mortgage Market Reform Dwight M. Jaffee Boothfinancial sector regulatory reform initiatives are currentlyset concerns bank regulatory reform as embedded in the 2010

  12. 2011 Revised Regulation FCOI Webinar for Grantees

    E-Print Network [OSTI]

    Bandettini, Peter A.

    2011 Revised Regulation FCOI Webinar for Grantees Provided by the National Institutes of Health Overview of Other Changes Grantee Institution Responsibilities Noncompliance Submitting FCOI Reports

  13. Risk, Uncertainty and Environmental Regulation 1. Introduction

    E-Print Network [OSTI]

    Callender, Craig

    and Silent Spring 3. Toxins 4. Regulations #12;"Poison is in everything, and no thing is without poison: plastics, refrigerants, esp. nylon. · DDT (Dichloro-Diphe

  14. Self-regulation in Self-Propelled Nematic Fluids

    E-Print Network [OSTI]

    Aparna Baskaran; M. Cristina Marchetti

    2012-04-15

    We consider the hydrodynamic theory of an active fluid of self-propelled particles with nematic aligning interactions. This class of materials has polar symmetry at the microscopic level, but forms macrostates of nematic symmetry. We highlight three key features of the dynamics. First, as in polar active fluids, the control parameter for the order-disorder transition, namely the density, is dynamically convected by active currents, resulting in a generic, model independent dynamical self-regulation that destabilizes the uniform nematic state near the mean-field transition. Secondly, curvature driven currents render the system unstable deep in the nematic state, as found previously. Finally, and unique to self-propelled nematics, nematic order induces local polar order that in turn leads to the growth of density fluctuations. We propose this as a possible mechanism for the smectic order of polar clusters seen in numerical simulations.

  15. Arsenic-induced alteration in intracellular calcium homeostasis induces head kidney macrophage apoptosis involving the activation of calpain-2 and ERK in Clarias batrachus

    SciTech Connect (OSTI)

    Banerjee, Chaitali [Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007 (India); Goswami, Ramansu [Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007 (India); Centre for Environmental Studies, Visva-Bharati University, Santiniketan 731 235 (India); Datta, Soma [Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007 (India); Rajagopal, R. [Gut Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007 (India); Mazumder, Shibnath, E-mail: shibnath1@yahoo.co.in [Immunobiology Laboratory, Department of Zoology, University of Delhi, Delhi 110 007 (India)

    2011-10-01

    We had earlier shown that exposure to arsenic (0.50 {mu}M) caused caspase-3 mediated head kidney macrophage (HKM) apoptosis involving the p38-JNK pathway in Clarias batrachus. Here we examined the roles of calcium (Ca{sup 2+}) and extra-cellular signal-regulated protein kinase (ERK), the other member of MAPK-pathway on arsenic-induced HKM apoptosis. Arsenic-induced HKM apoptosis involved increased expression of ERK and calpain-2. Nifedipine, verapamil and EGTA pre-treatment inhibited the activation of calpain-2, ERK and reduced arsenic-induced HKM apoptosis as evidenced from reduced caspase-3 activity, Annexin V-FITC-propidium iodide and Hoechst 33342 staining. Pre-incubation with ERK inhibitor U 0126 inhibited the activation of calpain-2 and interfered with arsenic-induced HKM apoptosis. Additionally, pre-incubation with calpain-2 inhibitor also interfered with the activation of ERK and inhibited arsenic-induced HKM apoptosis. The NADPH oxidase inhibitor apocynin and diphenyleneiodonium chloride also inhibited ERK activation indicating activation of ERK in arsenic-exposed HKM also depends on signals from NADPH oxidase pathway. Our study demonstrates the critical role of Ca{sup 2+} homeostasis on arsenic-induced HKM apoptosis. We suggest that arsenic-induced alteration in intracellular Ca{sup 2+} levels initiates pro-apoptotic ERK and calpain-2; the two pathways influence each other positively and induce caspase-3 mediated HKM apoptosis. Besides, our study also indicates the role of ROS in the activation of ERK pathway in arsenic-induced HKM apoptosis in C. batrachus. - Highlights: > Altered Ca{sup 2+} homeostasis leads to arsenic-induced HKM apoptosis. > Calpain-2 plays a critical role in the process. > ERK is pro-apoptotic in arsenic-induced HKM apoptosis. > Arsenic-induced HKM apoptosis involves cross talk between calpain-2 and ERK.

  16. Use of geostatistic techniques to describe a reservoir to be submitted into a secondary recovery process field case: {open_quotes}Eocene B-Inferior/VLG-3659, Ceuta, Venezuela{close_quotes}

    SciTech Connect (OSTI)

    Hernandez, T.; Poquioma, W.

    1997-08-01

    This study presents the results of an integrated reservoir study of the Eocene B-Inferior/VLG-3659, Area 7, Ceuta filed. This field located in the Maracaibo Lake in the western side of Venezuela. The objective was to evaluating the feasibility to implement a secondary recovery project by means of water flooding. Core information was used for this study (194 ft), PVT analysis, RFI, build-up and statistic`s pressure analysis, modem logs and production history data. Using geostatistical techniques (Kriging) it was defined a low uncertainty geological model that was validated by means of a black oil simulator (Eclipse). The results showed a good comparison of historical pressure of the reservoir against those obtained from the model, without the need of {open_quotes}history matching{close_quotes}. It means without modifying neither the initial rock properties nor reservoir fluids. The results of this study recommended drilling in two new locations, also the reactivation of four producing wells and water flooding under peripherical array by means of four injection wells, with the recovery of an additional 30.2 MMSTB. The economical evaluation shows an internal return rate of 31.4%.

  17. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ø.; Voulgarakis, Nikolaos K.

    2015-03-12

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore »melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  18. Balance of Irgm protein activities determines IFN--induced host defense

    E-Print Network [OSTI]

    Starnbach, Michael

    of IFN- regulated resistance to intracellular bacteria and protozoa. Among the IRG proteins, loss of Irgm1 has the most profound impact on IFN- -induced host resistance at the physiological level. Surpris- ingly, the losses of host resistance seen in the ab- sence of Irgm1 are sometimes more striking than

  19. ERK2: a logical AND gate critical for drug-induced plasticity? Truncated title

    E-Print Network [OSTI]

    1 Title: ERK2: a logical AND gate critical for drug-induced plasticity? Truncated title: ERK2 signal-regulated kinase (ERK) plays an important role in the underlying molecular mechanisms. ERK. Blockade of ERK activation prevents long- lasting behavioral changes, including psychomotor sensitization

  20. Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK/JNK-dependent activation of NF-jB in THP-1 cells

    E-Print Network [OSTI]

    Lee, Won-Ha

    Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK is mediated by mitogen activated protein kinases (MAPKs) including extracellular-signal-regulated kinase (ERK inducing molecule through interaction with its counterpart and Fas-mediate events are mediated by ERK

  1. Implementation Regulations 3TU MSc Sustainable Energy Technology Delft, 2015-2016 THE IMPLEMENTATION REGULATIONS

    E-Print Network [OSTI]

    van Vliet, Lucas J.

    Implementation Regulations 3TU MSc Sustainable Energy Technology ­ Delft, 2015-2016 THE IMPLEMENTATION REGULATIONS 2015 - 2016 3TU MASTER'S DEGREE PROGRAMME Sustainable Energy Technology - Delft (SET) DELFT UNIVERSITY OF TECHNOLOGY Administrative data Nomenclature in CROHO M Sustainable Energy Technology

  2. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B. (Chicago, IL); Hoek, Terry Vanden (Chicago, IL); Kasza, Kenneth E. (Palos Park, IL)

    2003-04-15

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  3. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B. (Chicago, IL); Hoek, Terry Vanden (Chicago, IL); Kasza, Kenneth E. (Palos Park, IL)

    2008-09-09

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  4. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2005-11-08

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  5. Electromagnetic Induced Gravitational Perturbations

    E-Print Network [OSTI]

    T. M. Adamo; E. T. Newman

    2008-07-23

    We study the physical consequences of two diffferent but closely related perturbation schemes applied to the Einstein-Maxwell equations. In one case the starting space-time is flat while in the other case it is Schwarzschild. In both cases the perturbation is due to a combined electric and magnetic dipole field. We can see, within the Einstein-Maxwell equations a variety of physical consequences. They range from induced gravitational energy-momentum loss, to a well defined spin angular momentum with its loss and a center-of-mass with its equations of motion.

  6. Transient Uncoupling Induces Synchronization

    E-Print Network [OSTI]

    Malte Schröder; Manu Mannattil; Debabrata Dutta; Sagar Chakraborty; Marc Timme

    2015-08-26

    Finding conditions that support synchronization is a fertile and active area of research with applications across multiple disciplines. Here we present and analyze a scheme for synchronizing chaotic dynamical systems by transiently uncoupling them. Specifically, systems coupled only in a fraction of their state space may synchronize even if fully coupled they do not. Although, for many standard systems, coupling strengths need to be bounded to ensure synchrony, transient uncoupling removes this bound and thus enables synchronization in an infinite range of effective coupling strengths. The presented coupling scheme thus opens up the possibility to induce synchrony in (biological or technical) systems whose parameters are fixed and cannot be modified continuously.

  7. Steam Condensation Induced Waterhammer 

    E-Print Network [OSTI]

    Kirsner, W.

    2000-01-01

    mer-- i.e. fast moving steam picking up a slug of condensate and hurling it downstream against an elbow or a valve. Condensation Induced Waterham mer can be 100 times more powerful than this type of waterhammer. Because it does not require flowing... to seek relief from the Owner. A compromise was negotiated after the first week- steam would be de-energized at midnight before each workday, asbestos abators would start work at 4:00 a.m. and finish by noontime at which time steam would be restored...

  8. Major Changes to the 1995 Regulations Topic 1995 Regulations 2011 Final Rule

    E-Print Network [OSTI]

    Major Changes to the 1995 Regulations Topic 1995 Regulations 2011 Final Rule Significant Financial was managed, reduced, or eliminated INITIAL REPORT Requirements in 1995 reg, plus: · Name of the entity for the #12;Topic 1995 Regulations 2011 Final Rule Institution's determination that the financial interest

  9. Academic regulations for the Master's degree in Journalism The framework provisions of the academic regulations

    E-Print Network [OSTI]

    Bataillon, Thomas

    Academic regulations for the Master's degree in Journalism The framework provisions of the academic regulations The academic regulations for the Bachelor's degree in Journalism (2007) were prepared by the Board's degree in Journalism entitles the holder to use the title of MA in Journalism (Master of Journalism

  10. Scope of the Export Administration Regulations Part 734-page 1 Export Administration Regulations August 2001

    E-Print Network [OSTI]

    Bernstein, Daniel

    Scope of the Export Administration Regulations Part 734-page 1 Export Administration Regulations August 2001 §734.1 INTRODUCTION (a) In this part, references to the Export Adminis- tration Regulations (EAR) are references to 15 CFR chapter VII, subchapter C. This part de- scribes the scope of the Export

  11. Code of Regulations State of California

    E-Print Network [OSTI]

    , Regulation, and Conservation of Oil and Gas Resources (includes Subchapter 4. Statewide Geothermal, and Geothermal Resources Elena M. Miller, State Oil and Gas Supervisor #12;CALIFORNIA CODE OF REGULATIONS California Department of Conservation Division of Oil, Gas, and Geothermal Resources January 2011 Sacramento

  12. UNIVERSITY OF FLORIDA MILTON CAMPUS PARKING REGULATIONS

    E-Print Network [OSTI]

    Ma, Lena

    of the vehicle must be visible from parking lot throughways at all times. 2. Student parking decals allow parkingUNIVERSITY OF FLORIDA MILTON CAMPUS PARKING REGULATIONS PARKING REGULATIONS 1. Backing into or pulling through a campus parking space is prohibited. The vehicle decal displayed on the rear

  13. Engineer Regulation 1110-2-1150

    E-Print Network [OSTI]

    US Army Corps of Engineers

    CECW-EP Engineer Regulation 1110-2-1150 Department of the Army U.S. Army Corps of Engineers Washington, DC 20314-1000 ER 1110-2-1150 31 August 1999 Engineering and Design ENGINEERING AND DESIGN 1110-2-1150 U.S. Army Corps of Engineers CECW-EP Washington, D.C. 20314-1000 Regulation No. 1110

  14. Regulation mechanisms in spatial stochastic development models

    E-Print Network [OSTI]

    Dmitri Finkelshtein; Yuri Kondratiev

    2008-09-04

    The aim of this paper is to analyze different regulation mechanisms in spatial continuous stochastic development models. We describe the density behavior for models with global mortality and local establishment rates. We prove that the local self-regulation via a competition mechanism (density dependent mortality) may suppress a unbounded growth of the averaged density if the competition kernel is superstable.

  15. Advanced Clean Cars Zero Emission Vehicle Regulation

    E-Print Network [OSTI]

    California at Davis, University of

    Advanced Clean Cars Zero Emission Vehicle Regulation ZEV #12;Advanced Clean Cars ZEV Program.4% of Annual Sales in 2025 Projected: ZEVs #12;Advanced Clean Cars Hydrogen Infrastructure · Without infrastructure, the cars won't come · Complementary Policies to support ZEV regulation ­ Clean Fuels Outlet

  16. Food Safety Regulations for Farmers'Markets

    E-Print Network [OSTI]

    safety regulations. Classification of a Farmers'Market Food safety issues are governed by the retail food for all retail food establishments,including farmers' markets; however,each local county health departmentFood Safety Regulations for Farmers'Markets Christa Hofmann and Jennifer Dennis Department

  17. Export Control Regulations Colorado School of Mines

    E-Print Network [OSTI]

    1 Export Control Regulations Colorado School of Mines Office of Research Administration Ralph from whom much of this material was adapted #12;2 What are Export Controls? US laws that regulate and information for reasons of foreign policy and national security. #12;3 US Export Controls and Responsible

  18. Oklahoma State University RULES AND REGULATIONS

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    Oklahoma State University RULES AND REGULATIONS Effective date: 8/1/2013 through 8/31/2014 What page i) Effective May 6, 2013 OrangeRide Bicycle Rental and Repair (see page 52) #12;OKLAHOMA STATE UNIVERSITY PARKING RULES AND REGULATIONS © 2014 Department of Parking and Transportation Services Oklahoma

  19. University Library AMSTERDAM UNIVERSITY LIBRARY USER REGULATIONS

    E-Print Network [OSTI]

    van Rooij, Robert

    University Library AMSTERDAM UNIVERSITY LIBRARY USER REGULATIONS Regulations for the users of the libraries of the University of Amsterdam General 1. The Amsterdam University Library (from hereon: the library) is understood to mean the whole body of faculty libraries and facilities of the Library

  20. Molecular Cell Hydroxyurea Induces Hydroxyl

    E-Print Network [OSTI]

    Collins, James J.

    Molecular Cell Article Hydroxyurea Induces Hydroxyl Radical-Mediated Cell Death in Escherichia coli superoxide production, together with the increased iron uptake, fuels the formation of hydroxyl radicals

  1. CHAPTER 11: CONTROLLING MANAGERS' `BECOMING': THE PRACTICE OF IDENTITY REGULATION

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 CHAPTER 11: CONTROLLING MANAGERS' `BECOMING': THE PRACTICE OF IDENTITY REGULATION Stéphan Pezé regulation. My focus is on how identity regulation is performed through individuals' concrete actions regulation discourses are performed through micro- practices showing that identity regulation is far from

  2. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: April 29, 2008 REGULATION TITLE approval FAU Regulation 4.013, Exceptional Circumstance Withdrawals. This regulation outlines the process, and staff when it is appropriate to use this regulation. The committee, composed of representatives from all

  3. Magnetically-Regulated Fragmentation Induced by Nonlinear Flows and Ambipolar Diffusion

    E-Print Network [OSTI]

    Basu, Shantanu

    that a power spectrum with equal energy on all scales also accelerates collapse, but by a lesser amount magnetic field causes a rebound from the initial compres- sion, and the system undergoes several

  4. pH-induced Structural Changes Regulate Histidine Decarboxylase Activity in Lactobacillus 30a

    E-Print Network [OSTI]

    Elisabeth Schelp, Scott Worley, Arthur F. Monzingo, Stephen Ernst and Jon D. Robertus* Institute of Cellular monomers and relatively little or no effect on quaternary structure. # 2001 Academic Press Keywords

  5. Torsional regulation of hRPA-induced unwinding of double-stranded DNA

    E-Print Network [OSTI]

    Dekker, Cees

    and mechanism of the unwinding and rewinding reaction through single-molecule experiments. Human RPA (h Department of Cell Biology and Genetics, Cancer Genomic Center and 3 Department of Radiation OncologyDNA. Here, we study the dynamics of human RPA (hRPA) activity on topolog- ically constrained ds

  6. microRNA-223 Regulates Macrophage Polarization and Diet-induced Insulin Resistance 

    E-Print Network [OSTI]

    Meng, Cong

    2013-05-01

    network for macrophage activation and adipose tissue function. We have examined the activity of microRNA-223 (miR-223) and its role in controlling macrophage functions in adipose tissue inflammation and systemic insulinresistance. miR-223-/- mice on a...

  7. Genetic regulation of dietary restriction-induced longevity in Caenorhabditis elegans

    E-Print Network [OSTI]

    Bishop, Nicholas A

    2007-01-01

    Dietary restriction (DR), the limitation of food intake below the ad libidum (AL) level without malnutrition, extends mean and maximum lifespan in every organism in which it has been tested. Perhaps even more significantly, ...

  8. Regulation of Notch by Itch AIP4/Itch Regulates Notch Receptor Degradation in the Absence of Ligand

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Regulation of Notch by Itch 1 AIP4/Itch Regulates Notch Receptor Degradation in the AbsenceS ONE 3, 7 (2008) e2735" #12;Regulation of Notch by Itch 2 ABSTRACT Background. The regulation of Notch of ubiquitin-ligases, has been described as a negative regulator of Notch signaling, acting on the post

  9. Induced Currents, Eddy Currents and

    E-Print Network [OSTI]

    Tobar, Michael

    Lecture 11 Induced Currents, Eddy Currents and Maxwell's Equations 1 Faraday Disk Dynamo F = q Eddy Currents We have learnt that changing magnetic fields can induce electric fields in conductors 108 ms-1 16 #12;In this lecture and the next Eddy Currents: We have covered Sect. 29

  10. Modeling microbially induced calcite precipitation

    E-Print Network [OSTI]

    Cirpka, Olaf Arie

    Modeling microbially induced calcite precipitation Erlangen, November 27th 2014 Johannes Hommel and storage uses require separation. sealing of leakage pathways is important sealing = mineral precipitation Induced Calcite Precipitation (MICP) is investigated as sealing technology. It is used as an exemplary

  11. Hazardous Waste Management Standards and Regulations (Kansas)

    Broader source: Energy.gov [DOE]

    This act states the standards and regulations for the management of hazardous waste. No person shall construct, modify or operate a hazardous waste facility or otherwise dispose of hazardous waste...

  12. Is Conservation a Legitimate Goal of Regulation

    E-Print Network [OSTI]

    Goble, G. L.

    1984-01-01

    of these other regulatory precepts. The discussion also provides a forum for reconciling conflicting regulatory goals. A logical basis for determining whether energy conservation is a legitimate goal of regulation is set forth for consideration....

  13. Creating Sustainable Regulation of the Open Internet

    E-Print Network [OSTI]

    Hantover, Lixian

    2013-01-01

    in the midst of a rapid Internet sea change. Every day, newfor any new regulation of the Internet is sustainability: toL.J. 1. 49 2 The Pew Internet and American Life project

  14. Adhesion gene regulation in mammary cell proliferation

    E-Print Network [OSTI]

    Lau, Eric HonYui

    2011-01-01

    A. , et al. , Nectin: an adhesion molecule involved in OF CALIFORNIA, MERCED Adhesion Gene Regulation in Mammary1 1.2 Role of Adhesion Molecules in Development and

  15. Essays on entry regulation, institutions, and development

    E-Print Network [OSTI]

    Bruhn, Miriam

    2007-01-01

    This thesis is a collection of three essays on entry regulation, institutions, and development.Chapter 1 examines the effect of a business registration reform in Mexico on economic activity. This reform made registration ...

  16. Regulating new construction in historic areas

    E-Print Network [OSTI]

    Sellers-Garcia, Oliver

    2006-01-01

    This study is an examination of how the restrictiveness of different design regulations impacts the process of new construction in historic areas. The North End, South End, and Back Bay neighborhoods of Boston were identified ...

  17. Self-Regulation as Two-Stage Rent-Seeking

    E-Print Network [OSTI]

    von Wangemheim, Georg

    2009-01-01

    interests and self-regulation; an economic approach (pp.Jose; Llobet, Gerard (2007): “Regulation, Corporate SocialA Signaling Motive for Self-Regulation in the Shadow of

  18. Emotion Regulation and Attachment: Unpacking Two Constructs and Their Association

    E-Print Network [OSTI]

    Waters, Sara F.; Virmani, Elita A.; Thompson, Ross A.; Meyer, Sara; Raikes, H. Abigail; Jochem, Rachel

    2010-01-01

    The socialization of emotion regulation in the family. In J.Handbook of emotion regulation (pp. 249–268). New York:J. L. (2002). Anger regulation in disadvantaged preschool

  19. Business or Pleasure? Utilitarian Versus Hedonic Considerations in Emotion Regulation

    E-Print Network [OSTI]

    Gross, James J.

    Business or Pleasure? Utilitarian Versus Hedonic Considerations in Emotion Regulation Maya Tamir, it is typically assumed that individuals regulate emotions to obtain hedonic, rather than utilitarian, benefits predicted motives for emotion regulation. When anticipating a threatening task, participants who viewed

  20. Emotion Regulation in Children and Adolescents With Autism Spectrum Disorder

    E-Print Network [OSTI]

    Gross, James J.

    Emotion Regulation in Children and Adolescents With Autism Spectrum Disorder Andrea C. Samson group- matched typically developing (TD) controls completed a Reactivity and Regulation Situation Task. This task assesses emotional reactivity and spontaneous use of emotion regulation strategies (problem

  1. Factories: The Shipbuilding and Ship-repairing Regulations 1960 

    E-Print Network [OSTI]

    Hare, John

    1960-01-01

    These Regulations revoke the Shipbuilding Regulations, 1931, which were designed for the safety, health and welfare of persons employed in the construction and repair of ships in sbipbuilding yards. The present Regulations, ...

  2. REGULATIONS ON PHOTOVOLTAIC MODULE DISPOSAL AND RECYCLING.

    SciTech Connect (OSTI)

    FTHENAKIS,V.

    2001-01-29

    Environmental regulations can have a significant impact on product use, disposal, and recycling. This report summarizes the basic aspects of current federal, state and international regulations which apply to end-of-life photovoltaic (PV) modules and PV manufacturing scrap destined for disposal or recycling. It also discusses proposed regulations for electronics that may set the ground of what is to be expected in this area in the near future. In the US, several states have started programs to support the recycling of electronic equipment, and materials destined for recycling often are excepted from solid waste regulations during the collection, transfer, storage and processing stages. California regulations are described separately because they are different from those of most other states. International agreements on the movement of waste between different countries may pose barriers to cross-border shipments. Currently waste moves freely among country members of the Organization of Economic Cooperation and Development (OECD), and between the US and the four countries with which the US has bilateral agreements. However, it is expected, that the US will adopt the rules of the Basel Convention (an agreement which currently applies to 128 countries but not the US) and that the Convection's waste classification system will influence the current OECD waste-handling system. Some countries adopting the Basel Convention consider end-of-life electronics to be hazardous waste, whereas the OECD countries consider them to be non-hazardous. Also, waste management regulations potentially affecting electronics in Germany and Japan are mentioned in this report.

  3. Air Pollution Control Regulations: No. 13 - Particulate Emissions...

    Broader source: Energy.gov (indexed) [DOE]

    Environmental Regulations Provider Department of Environmental Management The purpose of this regulation is to limit emissions of particulate matter from fossil fuel fired and...

  4. Air Pollution Control Regulations: No. 41 - Nox Budget Trading...

    Broader source: Energy.gov (indexed) [DOE]

    Savings Category Fuel Cells Program Info State Rhode Island Program Type Environmental Regulations Provider Department of Environmental Management These regulations establish a...

  5. Guide to Federal Regulation of Sales of Imported Electricity...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Guide to Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States Guide to Federal Regulation of Sales of Imported Electricity in Canada, Mexico...

  6. Transport Regulation from Theory to Practice: General Observations...

    Open Energy Info (EERE)

    Transport Regulation from Theory to Practice: General Observations and a Case Study Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Transport Regulation from Theory to...

  7. Guide to Federal Regulation of Sales of Imported Electricity...

    Office of Environmental Management (EM)

    Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States Guide to Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the...

  8. The Greenhouse Gases, Regulated Emissions, and Energy Use in...

    Open Energy Info (EERE)

    The Greenhouse Gases, Regulated Emissions, and Energy Use in Transportation Model (GREET) Jump to: navigation, search Tool Summary LAUNCH TOOL Name: The Greenhouse Gases, Regulated...

  9. Contacts for the Deputy General Counsel for Litigation, Regulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Litigation, Regulation and Enforcement (GC-30) Contacts for the Deputy General Counsel for Litigation, Regulation and Enforcement (GC-30) Anne Harkavy, Deputy General Counsel for...

  10. EA-1631: Beacon Power Corporation Frequency Regulation Facility...

    Office of Environmental Management (EM)

    1: Beacon Power Corporation Frequency Regulation Facility in Stephentown, NY EA-1631: Beacon Power Corporation Frequency Regulation Facility in Stephentown, NY February 2, 2009...

  11. How Much Do Local Regulations Matter? Exploring the Impact of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations Matter? Exploring the Impact of Permitting and Local Regulatory Processes on PV Prices in the United States How Much Do Local Regulations Matter? Exploring the Impact...

  12. The Nuclear Installations (Dangerous Occurrences )(Amendment) Regulations 1963 

    E-Print Network [OSTI]

    Wood, Richard; Noble, Michael

    1963-01-01

    STATUTORY INSTRUMENTS 1963 No. 1358 ATOMIC ENERGY AND RADIOACTIVE SUBSTANCES LICENSING AND REGULATION OF SITES The Nuclear Installations (Dangerous Occurrences) (Amendment) Regulations 1963...

  13. Update on Diesel Exhaust Emission Control Technology and Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Control Technology and Regulations Update on Diesel Exhaust Emission Control Technology and Regulations 2004 Diesel Engine Emissions Reduction (DEER) Conference Presentation:...

  14. POLICY FLASH 2015-22 - Federal Acquisition Regulation Class Deviation...

    Office of Environmental Management (EM)

    2 - Federal Acquisition Regulation Class Deviation POLICY FLASH 2015-22 - Federal Acquisition Regulation Class Deviation DATE: May 8, 2015 TO: Procurement DirectorsContracting...

  15. EO 13211: Regulations That Significantly Affect Energy Supply...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EO 13211: Regulations That Significantly Affect Energy Supply, Distribution, or Use EO 13211: Regulations That Significantly Affect Energy Supply, Distribution, or Use I am...

  16. Department of Energy Finalizes Regulations to Increase Energy...

    Energy Savers [EERE]

    Finalizes Regulations to Increase Energy Efficiency in New Federal Buildings by 30% Department of Energy Finalizes Regulations to Increase Energy Efficiency in New Federal...

  17. Regulation of the assembly and function of the nuclear pore

    E-Print Network [OSTI]

    Delmar, Valerie Anne

    2008-01-01

    negatively regulates nuclear membrane fusion and nuclearExcess transportin blocks nuclear membrane fusion in a Ran-regulating nuclear membrane fusion and pore assembly. (The

  18. Chapter 2 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    Chapter 2 of the Wyoming Public Service Commission Regulations: General Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  19. The procedures and regulation pertaining to the internal examiner First: Aim of the regulation pertaining to the internal examiner

    E-Print Network [OSTI]

    The procedures and regulation pertaining to the internal examiner First: Aim of the regulation pertaining to the internal examiner: The aims of the internal examiner regulation is: 1- To ensure meticulous

  20. Electromagnetically Induced Flows Michiel de Reus

    E-Print Network [OSTI]

    Vuik, Kees

    Electromagnetically Induced Flows in Water Michiel de Reus 8 maart 2013 () Electromagnetically Conclusion and future research () Electromagnetically Induced Flows 2 / 56 #12;1 Introduction 2 Maxwell Navier Stokes equations 5 Simulations 6 Conclusion and future research () Electromagnetically Induced

  1. Regulation of apoptosis in human cancer cells

    E-Print Network [OSTI]

    Lloyd, S. Julie-Ann (Simone Julie-Ann)

    2005-01-01

    Nitric oxide is postulated to protect cancer cells from the death-inducing effects of tumour necrosis factor alpha by S-nitrosating the active site cysteines, inhibiting cleavage of caspase-9. We aimed to test this hypothesis ...

  2. Signaling pathways that regulate cellular senescence

    E-Print Network [OSTI]

    Freund, Adam Mark

    2010-01-01

    M) p53 inactivation increases DDR signaling but not p38MAPKexpression does not induce a DDR. Cells were infected asthat DNA damage response (DDR) signaling is essential but

  3. The accumulations of HIF-1? and HIF-2? by JNK and ERK are involved in biphasic effects induced by different levels of arsenite in human bronchial epithelial cells

    SciTech Connect (OSTI)

    Xu, Yuan; Li, Yuan [Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China) [Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China); The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China); Li, Huiqiao [Qujing Center for Disease Control and Prevention, Qujing 655000, Yunnan (China)] [Qujing Center for Disease Control and Prevention, Qujing 655000, Yunnan (China); Pang, Ying; Zhao, Yue; Jiang, Rongrong; Shen, Lu [Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China) [Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China); The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China); Zhou, Jianwei; Wang, Xinru [The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China)] [The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China); Liu, Qizhan, E-mail: drqzliu@hotmail.com [Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China) [Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China); The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 210029, Jiangsu (China)

    2013-01-15

    The biphasic effects of arsenite, in which low levels of arsenite induce cell proliferation and high levels of arsenite induce DNA damage and apoptosis, apparently contribute to arsenite-induced carcinogenesis. However, the mechanisms underlying this phenomenon are not well understood. In this study, we investigated the effects of different levels of arsenite on cell proliferation, DNA damage and apoptosis as well as on signal transduction pathways in human bronchial epithelial (HBE) cells. Our results show that a low level of arsenite activates extracellular signal-regulated kinases (ERK), which probably mediate arsenite-inhibited degradation of ubiquitinated hypoxia-inducible factor-2? (HIF-2?) in HBE cells. ERK inhibition blocks cell proliferation induced by a low level of arsenite, in part via HIF-2?. In contrast, a high level of arsenite activates c-Jun N-terminal kinases (JNK), which provoke a response to suppress ubiquitinated HIF-1? degradation. Down-regulation of HIF-1? by inhibiting JNK, however, increases the DNA damage but decreases the apoptosis induced by a high level of arsenite. Thus, data in the present study suggest that the accumulations of HIF-1? and HIF-2? by JNK and ERK are involved in different levels of arsenite-induced biphasic effects, with low levels of arsenite inducing cell proliferation and high levels of arsenite inducing DNA damage and apoptosis in HBE cells. -- Highlights: ? Biphasic effects induced by different concentrations of arsenite. ? Different regulation of ERK or JNK signal pathway by arsenite. ? Different regulation of HIF1? or HIF 2? by arsenite.

  4. Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation

    SciTech Connect (OSTI)

    Reed, J.C.; Tsujimoto, Y.; Alpers, J.D.; Croce, C.M.; Nowell, P.C.

    1987-06-05

    The bcl-2 and c-myc proto-oncogenes are brought into juxtaposition with the immuno-globulin heavy chain locus in particular B-cell lymphomas, resulting in high levels of constitutive accumulation of their messenger RNAs. Precisely how the products of the bcl-2 and c-myc genes contribute to tumorigenesis is unknown, but observations that c-myc expression is rapidly induced in nonneoplastic lymphocytes upon stimulation of proliferation raise the possibility that this proto-oncogene is involved in the control of normal cellular growth. In addition to c-myc, the bcl-2 proto-oncogene also was expressed in normal human B and T lymphocytes after stimulation with appropriate mitogens. Comparison of the regulation of the expression of these proto-oncogenes demonstrated marked differences and provided evidence that, in contrast to c-myc, levels of bcl-2 messenger RNA are regulated primarily though transcriptional mechanisms. 10 references, 3 figures.

  5. Transcriptional regulation of human RANK ligand gene expression by E2F1

    SciTech Connect (OSTI)

    Hu Yan [Department of Stress Biology, Roswell Park Cancer Institute, Buffalo, NY 14263 (United States); Sun Meng [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Graduate Program of Pharmacology and Toxicology, University of California at Davis, Sacramento, CA 95817 (United States); Nadiminty, Nagalakshmi; Lou Wei [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Pinder, Elaine [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Graduate Program of Pharmacology and Toxicology, University of California at Davis, Sacramento, CA 95817 (United States); Gao, Allen C. [Department of Urology and Cancer Center, University of California at Davis, School of Medicine, 4860 Y Street, Suite 3500, Sacramento, CA 95817 (United States); Graduate Program of Pharmacology and Toxicology, University of California at Davis, Sacramento, CA 95817 (United States)], E-mail: acgao@ucdavis.edu

    2008-06-06

    Receptor activator of nuclear factor kappa B ligand (RANKL) is a critical osteoclastogenic factor involved in the regulation of bone resorption, immune function, the development of mammary gland and cardiovascular system. To understand the transcriptional regulation of RANKL, we amplified and characterized a 1890 bp 5'-flanking sequence of human RANKL gene (-1782 bp to +108 bp relative to the transcription start site). Using a series of deletion mutations of the 1890 bp RANKL promoter, we identified a 72 bp region (-172 to -100 bp) mediating RANKL basal transcriptional activity. Sequence analysis revealed a putative E2F binding site within this 72 bp region in the human RANKL promoter. Overexpression of E2F1 increased RANKL promoter activity, while down-regulation of E2F1 expression by small interfering RNA decreased RANKL promoter activity. RT-PCR and enzyme linked immunosorbent assays (ELISA) further demonstrated that E2F1 induced the expression of RANKL. Electrophoretic gel mobility shift assays (EMSA) and antibody competition assays confirmed that E2F1 proteins bind to the consensus E2F binding site in the RANKL promoter. Mutation of the E2F consensus binding site in the RANKL promoter profoundly reduced the basal promoter activity and abolished the transcriptional modulation of RANKL by E2F1. These results suggest that E2F1 plays an important role in regulating RANKL transcription through binding to the E2F consensus binding site.

  6. Differential regulation of cyclin-dependent kinase inhibitors in neuroblastoma cells

    SciTech Connect (OSTI)

    Qiao, Lan [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States) [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Department of Pharmaceutical Sciences, Jilin University, Changchun 130021 (China); Paul, Pritha; Lee, Sora [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States) [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Qiao, Jingbo [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States)] [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Wang, Yongsheng [Department of Pharmaceutical Sciences, Jilin University, Changchun 130021 (China)] [Department of Pharmaceutical Sciences, Jilin University, Changchun 130021 (China); Chung, Dai H., E-mail: dai.chung@vanderbilt.edu [Department of Pediatric Surgery, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232 (United States)

    2013-05-31

    Highlights: •GRP-R signaling differentially regulated the expression of p21 and p27. •Silencing GRP/GRP-R downregulated p21, while p27 expression was upregulated. •Inhibition of GRP/GRP-R signaling enhanced PTEN expression, correlative to the increased expression of p27. •PTEN and p27 co-localized in cytoplasm and silencing PTEN decreased p27 expression. -- Abstract: Gastrin-releasing peptide (GRP) and its receptor (GRP-R) are highly expressed in undifferentiated neuroblastoma, and they play critical roles in oncogenesis. We previously reported that GRP activates the PI3K/AKT signaling pathway to promote DNA synthesis and cell cycle progression in neuroblastoma cells. Conversely, GRP-R silencing induces cell cycle arrest. Here, we speculated that GRP/GRP-R signaling induces neuroblastoma cell proliferation via regulation of cyclin-dependent kinase (CDK) inhibitors. Surprisingly, we found that GRP/GRP-R differentially induced expressions of p21 and p27. Silencing GRP/GRP-R decreased p21, but it increased p27 expressions in neuroblastoma cells. Furthermore, we found that the intracellular localization of p21 and p27 in the nuclear and cytoplasmic compartments, respectively. In addition, we found that GRP/GRP-R silencing increased the expression and accumulation of PTEN in the cytoplasm of neuroblastoma cells where it co-localized with p27, thus suggesting that p27 promotes the function of PTEN as a tumor suppressor by stabilizing PTEN in the cytoplasm. GRP/GRP-R regulation of CDK inhibitors and tumor suppressor PTEN may be critical for tumoriogenesis of neuroblastoma.

  7. Rules and Regulations for Hazardous Waste Management (Rhode Island)

    Broader source: Energy.gov [DOE]

    These regulations establish permitting and operational requirements for hazardous waste facilities. They are designed to minimize...

  8. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: October 9, 2007 REGULATION Board of Governors ("BOG") promulgated new regulations that relate to new program termination (6C-8.012, Academic Program Termination). The Regulations require that each university board of trustees adopt

  9. Self-Regulation and Negotiated Agreements: Complements or Substitutes?

    E-Print Network [OSTI]

    Lyon, Thomas P.

    Self-Regulation and Negotiated Agreements: Complements or Substitutes? Thomas P. Lyon and John W the possibility of corporate self-regulation. Yet in practice firms may have incentives to self-regulate in order arises whether self-regulation and negotiated agreements are complements or substitutes. We present

  10. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: October 13, 2011 REGULATION-support organizations (DSOs) are governed by Florida Statute s. 1004.28 and Board of Governors Regulation 6 conditions, controls and requirements as each board deems appropriate for oversight. The proposed regulation

  11. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: February 11, 2011 REGULATION.009). SUMMARY: The University administration seeks approval to amend FAU Regulation 4.009, Major Medical Insurance Requirements for Foreign Students. This proposed amended regulation, renamed Health Insurance

  12. Vohs.indb 407 The Effects of Self-Regulation

    E-Print Network [OSTI]

    Reber, Paul J.

    Vohs.indb 407 The Effects of Self-Regulation on Social Relationships GRAINNE M. FITZSIMONS ELI J consequences of self-regulation? A large body of research within personality, organizational, and social psychology has demonstrated that self-regulation has significant consequences for the individual. Good self-regulators

  13. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: October 9, 2007 REGULATION: At its March 2007 meeting, the Florida Board of Governors ("BOG") promulgated new regulations that relate to new program authorization (6C-8.011, Academic Program Authorization). The Regulations require

  14. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: April 29, 2008 REGULATION TITLE for the proposed FAU Regulation 4.014, Medical Advisory Committee. This committee will advise and make to themselves or others in the University. Approval of this proposed regulation allows the University to have

  15. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: June 18, 2009 REGULATION TITLE of Governors is required to promulgate a regulation to implement the Textbook Affordability Act of 2008. The BOG promulgated Regulation 8.003 on March 26, 2009, which requires each Board of Trustees

  16. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    -1- FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: April 5, 2010 REGULATION of Academic Affairs seeks approval of the proposed FAU Regulation 4.002, Student Academic Grievance Procedures for Grade Reviews. Approval is dependent on the repeal of the current FAU Regulation 4.002 and the approval

  17. Regulation and Execution of Meiosis in Drosophh Males

    E-Print Network [OSTI]

    9 Regulation and Execution of Meiosis in Drosophh Males lean Maines and Steven Wasserm. Identification of Male Meiotic Mutant\\ IT. Regulation of the Meiotic Cell Divisions A. Entry into Meiosis B. Regulation of the Cell Cycle Machinery C. Coordination of Meio\\i\\ with Diflerentiation D. Regulation

  18. Emotion Regulation Deficits in Frontotemporal Lobar Degeneration and Alzheimer's Disease

    E-Print Network [OSTI]

    Levenson, Robert W.

    Emotion Regulation Deficits in Frontotemporal Lobar Degeneration and Alzheimer's Disease Madeleine instructed and spontaneous emotion regulation in patients with frontotemporal lobar degeneration (FTLD, N 32) unwarned without instructions to down-regulate, (b) warned without instructions to down-regulate, and (c

  19. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: April 14, 2011 REGULATION TITLE College of Medicine seeks approval of the proposed FAU Regulation 9.001, Faculty Practice Plan and administration of the Faculty Practice Plan. FULL TEXT OF THE REGULATION: The full text of the proposed

  20. FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION

    E-Print Network [OSTI]

    Richman, Fred

    FLORIDA ATLANTIC UNIVERSITY NOTICE OF PROPOSED NEW REGULATION Date: April 16, 2009 REGULATION TITLE AND NUMBER: Institutes and Centers (2.006). SUMMARY: Board of Governors Regulation 10.015(3), University with Board of Governors criteria. Proposed Regulation 2.006 responds to this requirement and provides

  1. NOTICE OF REGULATION REPEAL Date: July 1, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    Collector and PV Module UCF-8.003 Certification SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal because the material contained in this regulation has been updated and adopted as 6C7-8.008, F REGULATION REPEAL: UCF-8.003 Solar Thermal Collector and PV Module Certification. (1) The Center shall

  2. NOTICE OF REGULATION REPEAL Date: July 1, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    Collector and PV Module UCF-8.002 Testing Standards SUMMARY OF REGULATION REPEAL: This regulation is proposed for repeal because the material contained in this regulation has been updated and adopted as rule OF THE PROPOSED REGULATION REPEAL: UCF-8.002 Solar Thermal Collector and PV Module Testing Standards. (1

  3. The Smoke Control Area (Authorised Fuels) Regulations 1963 

    E-Print Network [OSTI]

    Joseph, Keith

    1963-01-01

    STATUTORY INSTRUMENTS 1963 No. 1275 CLEAN AIR The Smoke Control Areas (Authorised Fuels) Regulations 1963

  4. NOTICE OF PROPOSED REGULATION AMENDMENT Date: November 8, 2011

    E-Print Network [OSTI]

    Wu, Shin-Tson

    .: Solar Thermal Collector and PV Module Certification UCF-8.008 SUMMARY OF REGULATION: This regulation-mail: regulations@mail.ucf.edu FULL TEXT OF THE PROPOSED REGULATION AMENDMENT: UCF-8.008 Solar Thermal Collector to the Seller of a solar thermal collector that has met the requirements of FSEC Standard 101-10 and for which

  5. Environmental regulations handbook for enhanced oil recovery

    SciTech Connect (OSTI)

    Madden, M.P. [National Inst. for Petroleum and Energy Research, Bartlesville, OK (United States); Blatchford, R.P.; Spears, R.B. [Spears and Associates, Inc., Tulsa, OK (United States)

    1991-12-01

    This handbook is intended to assist owners and operators of enhanced oil recovery (EOR) operations in acquiring some introductory knowledge of the various state agencies, the US Environmental Protection Agency, and the many environmental laws, rules and regulations which can have jurisdiction over their permitting and compliance activities. It is a compendium of summarizations of environmental rules. It is not intended to give readers specific working details of what is required from them, nor can it be used in that manner. Readers of this handbook are encouraged to contact environmental control offices nearest to locations of interest for current regulations affecting them.

  6. Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras–Raf–ERK–Sp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: •Nestin expression in C6 glioma cells is induced by FGF-2. •Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. •The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. •The mRNA of FGFR1 and 3 are detected in C6 glioma cells. •Ras–Raf–ERK–Sp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFR–MAPK–ERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  7. Hedgehog pathway regulators influence cervical cancer cell proliferation, survival and migration

    SciTech Connect (OSTI)

    Samarzija, Ivana; Beard, Peter

    2012-08-17

    Highlights: Black-Right-Pointing-Pointer Unknown cellular mutations complement papillomavirus-induced carcinogenesis. Black-Right-Pointing-Pointer Hedgehog pathway components are expressed by cervical cancer cells. Black-Right-Pointing-Pointer Hedgehog pathway activators and inhibitors regulate cervical cancer cell biology. Black-Right-Pointing-Pointer Cell immortalization by papillomavirus and activation of Hedgehog are independent. -- Abstract: Human papillomavirus (HPV) infection is considered to be a primary hit that causes cervical cancer. However, infection with this agent, although needed, is not sufficient for a cancer to develop. Additional cellular changes are required to complement the action of HPV, but the precise nature of these changes is not clear. Here, we studied the function of the Hedgehog (Hh) signaling pathway in cervical cancer. The Hh pathway can have a role in a number of cancers, including those of liver, lung and digestive tract. We found that components of the Hh pathway are expressed in several cervical cancer cell lines, indicating that there could exists an autocrine Hh signaling loop in these cells. Inhibition of Hh signaling reduces proliferation and survival of the cervical cancer cells and induces their apoptosis as seen by the up-regulation of the pro-apoptotic protein cleaved caspase 3. Our results indicate that Hh signaling is not induced directly by HPV-encoded proteins but rather that Hh-activating mutations are selected in cells initially immortalized by HPV. Sonic Hedgehog (Shh) ligand induces proliferation and promotes migration of the cervical cancer cells studied. Together, these results indicate pro-survival and protective roles of an activated Hh signaling pathway in cervical cancer-derived cells, and suggest that inhibition of this pathway may be a therapeutic option in fighting cervical cancer.

  8. The cAMP Response Element Binding protein (CREB) is activated by Insulin-like Growth Factor-1 (IGF-1) and regulates myostatin gene expression in skeletal myoblast

    SciTech Connect (OSTI)

    Zuloaga, R. [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile)] [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile); Fuentes, E.N.; Molina, A. [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile) [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile); Interdisciplinary Center for Aquaculture Research (INCAR), Víctor Lamas 1290, PO Box 160-C, Concepción (Chile); Valdés, J.A., E-mail: jvaldes@unab.cl [Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago (Chile); Interdisciplinary Center for Aquaculture Research (INCAR), Víctor Lamas 1290, PO Box 160-C, Concepción (Chile)

    2013-10-18

    Highlights: •IGF-1 induces the activation of CREB via IGF-1R/PI3K/PLC signaling pathway. •Calcium dependent signaling pathways regulate myostatin gene expression. •IGF-1 regulates myostatin gene expression via CREB transcription in skeletal myoblast. -- Abstract: Myostatin, a member of the Transforming Growth Factor beta (TGF-?) superfamily, plays an important role as a negative regulator of skeletal muscle growth and differentiation. We have previously reported that IGF-1 induces a transient myostatin mRNA expression, through the activation of the Nuclear Factor of Activated T cells (NFAT) in an IP{sub 3}/calcium-dependent manner. Here we examined the activation of CREB transcription factor as downstream targets of IGF-1 during myoblast differentiation and its role as a regulator of myostatin gene expression. In cultured skeletal myoblast, IGF-1 induced the phosphorylation and transcriptional activation of CREB via IGF-1 Receptor/Phosphatidylinositol 3-Kinase (PI3K)/Phospholipase C gamma (PLC ?), signaling pathways. Also, IGF-1 induced calcium-dependent molecules such as Calmodulin Kinase II (CaMK II), Extracellular signal-regulated Kinases (ERK), Protein Kinase C (PKC). Additionally, we examined myostatin mRNA levels and myostatin promoter activity in differentiated myoblasts stimulated with IGF-1. We found a significant increase in mRNA contents of myostatin and its reporter activity after treatment with IGF-1. The expression of myostatin in differentiated myoblast was downregulated by the transfection of siRNA–CREB and by pharmacological inhibitors of the signaling pathways involved in CREB activation. By using pharmacological and genetic approaches together these data demonstrate that IGF-1 regulates the myostatin gene expression via CREB transcription factor during muscle cell differentiation.

  9. Regulation No. Department of the Army

    E-Print Network [OSTI]

    US Army Corps of Engineers

    authorities and storm sewer systems to be accomplished by local interests. 2. Applicability. This regulation waters due to severe storms. c. "Storm sewer systems" are the facilities in urban areas designed, sewers and sewer appurtenances, on-site storage and detention basins, curbs and gutters, and other small

  10. NONPROLIFERATION PROMOTED BY INDUSTRY SELF-REGULATION

    E-Print Network [OSTI]

    , Mohamed Elbaradei's calls for an increased role of the nuclear industry in combating nuclear proliferation Mohamed Elbaradei's call for an increased role by the nuclear industry to combat nuclear proliferation). The need for an industry self-regulation approach results from the nexus between terrorism and nuclear

  11. Regulation No. Department of the Army

    E-Print Network [OSTI]

    US Army Corps of Engineers

    , DC 20314-1000 ER 1130-2-510 12 Dec 96 Project Operations HYDROELECTRIC POWER OPERATIONS.C. 20314-1000 Project Operations ER 1130-2-510 Change 1 16 December 2002 HYDROELECTRIC POWER OPERATIONS-OM Washington, D.C. 20314-1000 Regulation No. 1130-2-510 13 December 1996 Project Operations HYDROELECTRIC POWER

  12. Regulation No. 1110-2-1806

    E-Print Network [OSTI]

    US Army Corps of Engineers

    for the seismic design and evaluation for all civil works projects. 2. Applicability This regulation is applicable design for new projects and the seismic evaluation or reevaluation for existing projects should are not critical features. b. Design. Seismic design for new projects shall include assessments of the potential

  13. Commitments to Regulate Social Web Services Operation

    E-Print Network [OSTI]

    Sheng, Michael

    Commitments to Regulate Social Web Services Operation Zakaria Maamar, Noura Faci, Khouloud Boukadi, Quan Z. Sheng, Member, IEEE, and Lina Yao Abstract--This paper discusses how social Web services are held responsible for the actions they take at run time. Compared to (regular) Web services, social Web

  14. Voltage regulation in linear induction accelerators

    DOE Patents [OSTI]

    Parsons, W.M.

    1992-12-29

    Improvement in voltage regulation in a linear induction accelerator wherein a varistor, such as a metal oxide varistor, is placed in parallel with the beam accelerating cavity and the magnetic core is disclosed. The non-linear properties of the varistor result in a more stable voltage across the beam accelerating cavity than with a conventional compensating resistance. 4 figs.

  15. Voltage regulation in linear induction accelerators

    DOE Patents [OSTI]

    Parsons, William M. (Santa Fe, NM)

    1992-01-01

    Improvement in voltage regulation in a Linear Induction Accelerator wherein a varistor, such as a metal oxide varistor, is placed in parallel with the beam accelerating cavity and the magnetic core. The non-linear properties of the varistor result in a more stable voltage across the beam accelerating cavity than with a conventional compensating resistance.

  16. Method for regulation of plant lignin composition

    DOE Patents [OSTI]

    Chapple, Clint (West Lafayette, IN)

    1999-01-01

    A method is disclosed for the regulation of lignin composition in plant tissue. Plants are transformed with a gene encoding an active F5H gene. The expression of the F5H gene results in increased levels of syringyl monomer providing a lignin composition more easily degraded with chemicals and enzymes.

  17. TGS COMMONS REGULATIONS & POLICIES FOR RESERVED EVENTS

    E-Print Network [OSTI]

    TGS COMMONS REGULATIONS & POLICIES FOR RESERVED EVENTS Hour of Operation 8:00am-12:00am TGS the Registrar's 25 Live Scheduling System (Link available on the TGS Commons webpage). Students will also be required to fill out TGS-related forms before reservation confirmations will be sent. The Graduate Student

  18. Coping with uncertainties of mercury regulation

    SciTech Connect (OSTI)

    Reich, K.

    2006-09-15

    The thermometer is rising as coal-fired plants cope with the uncertainties of mercury regulation. The paper deals with a diagnosis and a suggested cure. It describes the state of mercury emission rules in the different US states, many of which had laws or rules in place before the Clean Air Mercury Rule (CAMR) was promulgated.

  19. Neurofibromin Regulation of ERK Signaling Modulates

    E-Print Network [OSTI]

    Silva, Alcino

    Neurofibromin Regulation of ERK Signaling Modulates GABA Release and Learning Yijun Cui,1 Rui M@mednet.ucla.edu DOI 10.1016/j.cell.2008.09.060 SUMMARY We uncovered a role for ERK signaling in GABA re- lease, long demonstrate that neurofibromin modulates ERK/synapsin I-dependent GABA re- lease, which in turn modulates

  20. Public utility regulation and national energy policy

    SciTech Connect (OSTI)

    Navarro, P.

    1980-09-01

    The linkage between Public Utility Commission (PUC) regulation, the deteriorating financial health of the electric utility industry, and implementation of national energy policy, particularly the reduction of foreign petroleum consumption in the utility sector is examined. The role of the Nation's utilities in the pursuit of national energy policy goals and postulates a linkage between PUC regulation, the poor financial health of the utility industry, and the current and prospective failure to displace foreign petroleum in the utility sector is discussed. A brief history of PUC regulation is provided. The concept of regulatory climate and how the financial community has developed a system of ranking regulatory climate in the various State jurisdictions are explained. The existing evidence on the hypothesis that the cost of capital to a utility increases and its availability is reduced as regulatory climate grows more unfavorable from an investor's point of view is analyzed. The implications of this cost of capital effect on the electric utilities and collaterally on national energy policy and electric ratepayers are explained. Finally various State, regional and Federal regulatory responses to problems associated with PUC regulation are examined.

  1. MicroRNA Regulation of IGF-1 

    E-Print Network [OSTI]

    McEnery, Kayla Anne Filan

    2013-02-04

    an incredible learning experience. Finally, I would like to thank my friends and family for their continuous love and support. 4 ABSTRACT MicroRNA Regulation of IGF. (May 2013) Kayla Anne Filan McEnery Department of Biochemistry & Biophysics Texas A...

  2. LIBRARY REGULATIONS 1. THE LIBRARY POLICY COMMITTEE

    E-Print Network [OSTI]

    Schellekens, Michel P.

    1 LIBRARY REGULATIONS 1. THE LIBRARY POLICY COMMITTEE 1.1 The Library Policy Committee shall shall be: [i] to formulate proposals in regard to policy matters relating to the Library for consideration by the Academic Council and the Governing Body; [ii] to monitor the implementation of Library

  3. The effect of praying on emotion regulation 

    E-Print Network [OSTI]

    Kossurok, Anke

    2012-11-28

    Regulation of Others and Self (EROS) scale, the Satisfaction with Life scale (SwLS) and the Positive Affect and Negative Affect Scale (PANAS-X) before as well as after engaging in prayer, coping or neutral activities for a period of one week. Outcomes showed...

  4. Regional Focus on GM Crop Regulation

    E-Print Network [OSTI]

    Church, George M.

    Regional Focus on GM Crop Regulation THE RECENT MEDIA COVERAGE OF THE DEVEL- opments in Brazil for com- mercial genetically modified (GM) crops in both the scientific and regulatory arena. The release of GM crops in these coun- tries might result in the unintentional entry of GM seeds into neighboring

  5. LP3853/LP3856 3A Fast Response Ultra Low Dropout Linear Regulators

    E-Print Network [OSTI]

    Berns, Hans-Gerd

    terminator n Post regulators n High efficiency linear regulators n Battery chargers n Other battery powered

  6. Phenylbutyric acid protects against carbon tetrachloride-induced hepatic fibrogenesis in mice

    SciTech Connect (OSTI)

    Wang, Jian-Qing [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China) [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China); Second Affiliated Hospital, Anhui Medical University, Hefei 230601 (China); Chen, Xi [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China)] [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China); Zhang, Cheng [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); Tao, Li [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China)] [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China); Zhang, Zhi-Hui; Liu, Xiao-Qian [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); Xu, Yuan-Bao [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China) [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China); Wang, Hua [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); Li, Jun, E-mail: lijun@ahmu.edu.cn [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China)] [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China); Xu, De-Xiang, E-mail: xudex@126.com [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)

    2013-01-15

    A recent report showed that the unfolded protein response (UPR) signaling was activated in the pathogenesis of carbon tetrachloride (CCl{sub 4})-induced hepatic fibrosis. Phenylbutyric acid (PBA) is a well-known chemical chaperone that inhibits endoplasmic reticulum (ER) stress and unfolded protein response (UPR) signaling. In the present study, we investigated the effects of PBA on CCl{sub 4}-induced hepatic fibrosis in mice. All mice were intraperitoneally (i.p.) injected with CCl{sub 4} (0.15 ml/kg BW, twice per week) for 8 weeks. In CCl{sub 4} + PBA group, mice were i.p. injected with PBA (150 mg/kg, twice per day) from the beginning of CCl{sub 4} injection to the end. As expected, PBA significantly attenuated CCl{sub 4}-induced hepatic ER stress and UPR activation. Although PBA alleviated, only to a less extent, hepatic necrosis, it obviously inhibited CCl{sub 4}-induced tumor necrosis factor alpha (TNF-?) and transforming growth factor beta (TGF-?). Moreover, PBA inhibited CCl{sub 4}-induced hepatic nuclear factor kappa B (NF-?B) p65 translocation and extracellular signal-regulated kinase (ERK) and c-Jun N-terminal Kinase (JNK) phosphorylation. Interestingly, CCl{sub 4}-induced ?-smooth muscle actin (?-SMA), a marker for the initiation phase of HSC activation, was significantly attenuated in mice pretreated with PBA. Correspondingly, CCl{sub 4}-induced hepatic collagen (Col)1?1 and Col1?2, markers for the perpetuation phase of HSC activation, were inhibited in PBA-treated mice. Importantly, CCl{sub 4}-induced hepatic fibrosis, as determined using Sirius red staining, was obviously attenuated by PBA. In conclusion, PBA prevents CCl{sub 4}-induced hepatic fibrosis through inhibiting hepatic inflammatory response and HSC activation. Highlights: ? CCl{sub 4} induces hepatic ER stress, inflammation, HSC activation and hepatic fibrosis. ? PBA alleviates CCl{sub 4}-induced hepatic ER stress and UPR signaling activation. ? PBA inhibits CCl{sub 4}-induced hepatic NF-?B activation and ERK and JNK phosphorylation. ? PBA effectively protects against CCl{sub 4}-induced HSC activation and hepatic fibrosis. ? ER stress is involved in CCl{sub 4}-induced hepatic inflammation and fibrogenesis.

  7. Static behaviour of induced seismicity

    E-Print Network [OSTI]

    Mignan, Arnaud

    2015-01-01

    The standard paradigm to describe seismicity induced by fluid injection is to apply nonlinear diffusion dynamics in a poroelastic medium. I show that the spatiotemporal behaviour and rate evolution of induced seismicity can, instead, be expressed by geometric operations on a static stress field produced by volume change at depth. I obtain laws similar in form to the ones derived from poroelasticity while requiring a lower description length. Although fluid flow is known to occur in the ground, it is not pertinent to the behaviour of induced seismicity. The proposed model is equivalent to the static stress model for tectonic foreshocks generated by the Non- Critical Precursory Accelerating Seismicity Theory. This study hence verifies the explanatory power of this theory outside of its original scope.

  8. Atomic Energy and Radioactive Substances: Licensing and Regulation of Sites: The Nuclear Installations Regulations, 1962 

    E-Print Network [OSTI]

    Wood, Richard; Maclay, John. S.

    1962-01-01

    These regulations, made by the Minister of Power and the Secretary of State for Scotland acting jointly, prescribe for the purpose of subsection (1)(b) of section one of the Nuclear Installations (Licensing and Insurance) ...

  9. SirT1 regulates bone mass in vivo through regulation of osteoblast and osteoclast differentiation

    E-Print Network [OSTI]

    Zainabadi, Kayvan

    2009-01-01

    As mammals age, osteoblast numbers decrease while osteoclast numbers increase, resulting in age-related or post-menopausal osteoporosis. SirT1, the mammalian orthologue of yeast Sir2, has been shown to be a negative regulator ...

  10. Interacting Induced Dark Energy Model

    E-Print Network [OSTI]

    Bahrehbakhsh, Amir F

    2016-01-01

    Similar to the idea of the brane world scenarios, but based on the approach of the induced matter theory, for a non--vacuum five--dimensional version of general relativity, we propose a model in which the conventional matter sources considered as all kind of the matter (the baryonic and dark) and the induced terms emerging from the extra dimension supposed to be as dark energy. Then we investigate the FLRW type cosmological equations and illustrate that the model is capable to explain respectively the deceleration and then acceleration eras of the universe expansion with an interacting term between the matter and dark energy.

  11. Graphene with geometrically induced vorticity

    E-Print Network [OSTI]

    Jiannis K. Pachos; Michael Stone; Kristan Temme

    2008-03-26

    At half filling, the electronic structure of graphene can be modelled by a pair of free two-dimensional Dirac fermions. We explicitly demonstrate that in the presence of a geometrically induced gauge field, an everywhere-real Kekule modulation of the hopping matrix elements can correspond to a non-real Higgs field with non-trivial vorticity. This provides a natural setting for fractionally charged vortices with localized zero modes. For fullerene-like molecules we employ the index theorem to demonstrate the existence of six low-lying states that do not depend strongly on the Kekule-induced mass gap.

  12. Radioadaptive Response Induced by Alpha-Particle-Induced Stress

    E-Print Network [OSTI]

    Yu, Peter K.N.

    of radioadaptive response (RAR) by com- munication of radiation-induced bystander signals. RAR is a kind of low-dose in the unirradiated embryos. Introduction Recent research have demonstrated the communication of radiation radiation effect, which occurs when a small preceding priming dose decreases the biological effectiveness

  13. Mechano-growth factor induces migration of rat mesenchymal stem cells by altering its mechanical properties and activating ERK pathway

    SciTech Connect (OSTI)

    Wu, Jiamin; Wu, Kewen; Lin, Feng; Luo, Qing; Yang, Li; Shi, Yisong [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China)] [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China); Song, Guanbin, E-mail: song@cqu.edu.cn [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China)] [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China); Sung, Kuo-Li Paul [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China) [Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044 (China); Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412 (United States)

    2013-11-08

    Highlights: •MGF induced the migration of rat MSC in a concentration-dependent manner. •MGF enhanced the mechanical properties of rMSC in inducing its migration. •MGF activated the ERK 1/2 signaling pathway of rMSC in inducing its migration. •rMSC mechanics may synergy with ERK 1/2 pathway in MGF-induced rMSC migration. -- Abstract: Mechano-growth factor (MGF) generated by cells in response to mechanical stimulation has been identified as a mechano effector molecule, playing a key role in regulating mesenchymal stem cell (MSC) function, including proliferation and migration. However, the mechanism(s) underlying how MGF-induced MSC migration occurs is still unclear. In the present study, MGF motivated migration of rat MSCs (rMSCs) in a concentration-dependent manner and optimal concentration of MGF at 50 ng/mL (defined as MGF treatment in this paper) was demonstrated. Notably, enhancement of mechanical properties that is pertinent to cell migration, such as cell traction force and cell stiffness were found to respond to MGF treatment. Furthermore, MGF increased phosphorylation of extracellular signal-regulated kinase (ERK), ERK inhibitor (i.e., PD98059) suppressed ERK phosphorylation, and abolished MGF-induced rMSC migration were found, demonstrating that ERK is involved molecule for MGF-induced rMSC migration. These in vitro evidences of MGF-induced rMSC migration and its direct link to altering rMSC mechanics and activating the ERK pathway, uncover the underlying biomechanical and biological mechanisms of MGF-induced rMSC migration, which may help find MGF-based application of MSC in clinical therapeutics.

  14. Liver X Receptor (LXR) activation negatively regulates visfatin expression in macrophages

    SciTech Connect (OSTI)

    Mayi, Therese Hervee; Rigamonti, Elena; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Pattou, Francois; Department of Endocrine Surgery, University Hospital, Lille; U859 Biotherapies for Diabetes, INSERM, Lille ; Staels, Bart; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Chinetti-Gbaguidi, Giulia; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille

    2011-01-07

    Research highlights: {yields} Synthetic LXR ligands decreased visfatin expression in human macrophages. {yields} LXR activation leads to a modest and transient decrease of NAD{sup +} concentration. {yields} LXR activation decreased PPAR{gamma}-induced visfatin in human macrophages. -- Abstract: Adipose tissue macrophages (ATM) are the major source of visfatin, a visceral fat adipokine upregulated during obesity. Also known to play a role in B cell differentiation (pre-B cell colony-enhancing factor (PBEF)) and NAD biosynthesis (nicotinamide phosphoribosyl transferase (NAMPT)), visfatin has been suggested to play a role in inflammation. Liver X Receptor (LXR) and Peroxisome Proliferator-Activated Receptor (PPAR){gamma} are nuclear receptors expressed in macrophages controlling the inflammatory response. Recently, we reported visfatin as a PPAR{gamma} target gene in human macrophages. In this study, we examined whether LXR regulates macrophage visfatin expression. Synthetic LXR ligands decreased visfatin gene expression in a LXR-dependent manner in human and murine macrophages. The decrease of visfatin mRNA was paralleled by a decrease of protein secretion. Consequently, a modest and transient decrease of NAD{sup +} concentration was observed. Interestingly, LXR activation decreased the PPAR{gamma}-induced visfatin gene and protein secretion in human macrophages. Our results identify visfatin as a gene oppositely regulated by the LXR and PPAR{gamma} pathways in human macrophages.

  15. cAMP-response-element-binding protein positively regulates breast cancer metastasis and subsequent bone destruction

    SciTech Connect (OSTI)

    Son, Jieun; Lee, Jong-Ho; Kim, Ha-Neui; Ha, Hyunil Lee, Zang Hee

    2010-07-23

    Research highlights: {yields} CREB is highly expressed in advanced breast cancer cells. {yields} Tumor-related factors such as TGF-{beta} further elevate CREB expression. {yields} CREB upregulation stimulates metastatic potential of breast cancer cells. {yields} CREB signaling is required for breast cancer-induced bone destruction. -- Abstract: cAMP-response-element-binding protein (CREB) signaling has been reported to be associated with cancer development and poor clinical outcome in various types of cancer. However, it remains to be elucidated whether CREB is involved in breast cancer development and osteotropism. Here, we found that metastatic MDA-MB-231 breast cancer cells exhibited higher CREB expression than did non-metastatic MCF-7 cells and that CREB expression was further increased by several soluble factors linked to cancer progression, such as IL-1, IGF-1, and TGF-{beta}. Using wild-type CREB and a dominant-negative form (K-CREB), we found that CREB signaling positively regulated the proliferation, migration, and invasion of MDA-MB-231 cells. In addition, K-CREB prevented MDA-MB-231 cell-induced osteolytic lesions in a mouse model of cancer metastasis. Furthermore, CREB signaling in cancer cells regulated the gene expression of PTHrP, MMPs, and OPG, which are closely involved in cancer metastasis and bone destruction. These results indicate that breast cancer cells acquire CREB overexpression during their development and that this CREB upregulation plays an important role in multiple steps of breast cancer bone metastasis.

  16. Diffusion, dimensionality and noise in transcriptional regulation

    E-Print Network [OSTI]

    Gasper Tkacik; William Bialek

    2007-12-12

    The precision of biochemical signaling is limited by randomness in the diffusive arrival of molecules at their targets. For proteins binding to the specific sites on the DNA and regulating transcription, the ability of the proteins to diffuse in one dimension by sliding along the length of the DNA, in addition to their diffusion in bulk solution, would seem to generate a larger target for DNA binding, consequently reducing the noise in the occupancy of the regulatory site. Here we show that this effect is largely cancelled by the enhanced temporal correlations in one dimensional diffusion. With realistic parameters, sliding along DNA has surprisingly little effect on the physical limits to the precision of transcriptional regulation.

  17. HIF-1 and NDRG2 contribute to hypoxia-induced radioresistance of cervical cancer Hela cells

    SciTech Connect (OSTI)

    Liu, Junye [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China) [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China); Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China); Zhang, Jing [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China)] [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China); Wang, Xiaowu [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China)] [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China); Li, Yan [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China)] [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China); Chen, Yongbin; Li, Kangchu [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China)] [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China); Zhang, Jian [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China)] [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China); Yao, Libo, E-mail: bioyao@fmmu.edu.cn [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China)] [Department of Biochemistry and Molecular Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an (China); Guo, Guozhen, E-mail: guozhengg@hotmail.com [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China)] [Department of Radiation Medicine, Fourth Military Medical University, Xi'an (China)

    2010-07-15

    Hypoxia inducible factor 1 (HIF-1), the key mediator of hypoxia signaling pathways, has been shown involved in hypoxia-induced radioresistance. However, the underlying mechanisms are unclear. The present study demonstrated that both hypoxia and hypoxia mimetic cobalt chloride could increase the radioresistance of human cervical cancer Hela cells. Meanwhile, ectopic expression of HIF-1 could enhance the resistance of Hela cells to radiation, whereas knocking-down of HIF-1 could increase the sensitivity of Hela cells to radiation in the presence of hypoxia. N-Myc downstream-regulated gene 2 (NDRG2), a new HIF-1 target gene identified in our lab, was found to be upregulated by hypoxia and radiation in a HIF-1-dependent manner. Overexpression of NDRG2 resulted in decreased sensitivity of Hela cells to radiation while silencing NDRG2 led to radiosensitization. Moreover, NDRG2 was proved to protect Hela cells from radiation-induced apoptosis and abolish radiation-induced upregulation of Bax. Taken together, these data suggest that both HIF-1 and NDRG2 contribute to hypoxia-induced tumor radioresistance and that NDRG2 acts downstream of HIF-1 to promote radioresistance through suppressing radiation-induced Bax expression. It would be meaningful to further explore the clinical application potential of HIF-1 and NDRG2 blockade as radiosensitizer for tumor therapy.

  18. Spred2 is involved in imatinib-induced cytotoxicity in chronic myeloid leukemia cells

    SciTech Connect (OSTI)

    Liu, Xiao-Yun; Yang, Yue-Feng; Wu, Chu-Tse; Xiao, Feng-Jun; Zhang, Qun-Wei [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Ma, Xiao-Ni [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Li, Qing-Fang; Yan, Jun [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Wang, Hua, E-mail: wanghualjh@gmail.com [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Wang, Li-Sheng, E-mail: wangls@nic.bmi.ac.cn [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)

    2010-03-19

    Spreds, a recently established class of negative regulators of the Ras-ERK (extracellular signal-regulated kinase) pathway, are involved in hematogenesises, allergic disorders and tumourigenesis. However, their role in hematologic neoplasms is largely unknown. Possible effects of Spreds on other signal pathways closely related to Ras-ERK have been poorly investigated. In this study, we investigated the in vitro effects of Spred2 on chronic myeloid leukemia (CML) cells. In addition to inhibiting the well-established Ras-ERK cascade, adenovirus-mediated Spred2 over-expression inhibits constitutive and stem cell factor (SCF)-stimulated sphingosine kinase-1 (SPHK1) and Mcl-1 expression, as well as inhibiting proliferation and inducing apoptosis in CML cells. In K562 cells and primary CML cells, imatinib induces endogenous Spred2 expression. Spred2 silencing by stable RNA interference partly protects K562 cells against imatinib-induced apoptosis. Together, these data implicate Spred2 in imatinib-induced cytotoxicity in CML cells, possibly by inhibiting the Ras-ERK cascade and the pro-survival signaling molecules SPHK1 and Mcl-1. These findings reveal potential targets for selective therapy of CML.

  19. Regulation of flexible arms under gravity

    SciTech Connect (OSTI)

    De Luca, A.; Siciliano, B.

    1993-08-01

    A simple controller is presented for the regulation problem of robot arms with flexible links under gravity. It consists of a joint PD feedback plus a constant feedforward. Global asymptotic stability of the reference equilibrium state is shown under a structural assumption about link elasticity and a mild condition on the proportional gain. The result holds also in the absence of internal damping of the flexible arm. A numerical case study is presented.

  20. International Electricity Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuelsof EnergyApril 2014 | DepartmentDepartmentInternational CooperationRegulation

  1. Induced geometry from disformal transformation

    E-Print Network [OSTI]

    Fang-Fang Yuan; Peng Huang

    2015-02-08

    In this note, we use the disformal transformation to induce a geometry from the manifold which is originally Riemannian. The new geometry obtained here can be considered as a generalization of Weyl integrable geometry. Based on these results, we further propose a geometry which is naturally a generalization of Weyl geometry.

  2. Linear noise approximation of noise-induced oscillation in NF-?B signaling network

    E-Print Network [OSTI]

    Jaewook Joo

    2010-10-08

    NF-{\\kappa}B, one of key regulators of inflammation, apoptosis, and differentiation, was found to have noisy oscillatory shuttling between the nucleus and the cytoplasm in single cells when cells are stimulated by cytokine TNF{\\alpha}. We present the analytical analysis which uncovers the underlying physical mechanisms of this spectacular noise-induced transition in biological networks. Starting with the master equation describing both signaling and transcription events in NF-{\\kappa}B signaling network, we derived the macroscopic and the Fokker-Planck equations by using van Kampen's sysem size expansion. Using the noise-induced oscillatory signatures present in the power spectrum, we constructed the two-dimensional phase diagram where the noise-induced oscillation emerges in the dynamically stable parameter space.

  3. Rac1 and Cdc42 GTPases regulate shear stress-driven ?-catenin signaling in osteoblasts

    SciTech Connect (OSTI)

    Wan, Qiaoqiao; Cho, Eunhye; Yokota, Hiroki; Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN 46202 ; Na, Sungsoo

    2013-04-19

    Highlights: •Shear stress increased TCF/LEF activity and stimulated ?-catenin nuclear localization. •Rac1, Cdc42, and RhoA displayed distinct dynamic activity patterns under flow. •Rac1 and Cdc42, but not RhoA, regulate shear stress-driven TCF/LEF activation. •Cytoskeleton did not significantly affect shear stress-induced TCF/LEF activation. -- Abstract: Beta-catenin-dependent TCF/LEF (T-cell factor/lymphocyte enhancing factor) is known to be mechanosensitive and an important regulator for promoting bone formation. However, the functional connection between TCF/LEF activity and Rho family GTPases is not well understood in osteoblasts. Herein we investigated the molecular mechanisms underlying oscillatory shear stress-induced TCF/LEF activity in MC3T3-E1 osteoblast cells using live cell imaging. We employed fluorescence resonance energy transfer (FRET)-based and green fluorescent protein (GFP)-based biosensors, which allowed us to monitor signal transduction in living cells in real time. Oscillatory (1 Hz) shear stress (10 dynes/cm{sup 2}) increased TCF/LEF activity and stimulated translocation of ?-catenin to the nucleus with the distinct activity patterns of Rac1 and Cdc42. The shear stress-induced TCF/LEF activity was blocked by the inhibition of Rac1 and Cdc42 with their dominant negative mutants or selective drugs, but not by a dominant negative mutant of RhoA. In contrast, constitutively active Rac1 and Cdc42 mutants caused a significant enhancement of TCF/LEF activity. Moreover, activation of Rac1 and Cdc42 increased the basal level of TCF/LEF activity, while their inhibition decreased the basal level. Interestingly, disruption of cytoskeletal structures or inhibition of myosin activity did not significantly affect shear stress-induced TCF/LEF activity. Although Rac1 is reported to be involved in ?-catenin in cancer cells, the involvement of Cdc42 in ?-catenin signaling in osteoblasts has not been identified. Our findings in this study demonstrate that both Rac1 and Cdc42 GTPases are critical regulators in shear stress-driven ?-catenin signaling in osteoblasts.

  4. RhoE interferes with Rb inactivation and regulates the proliferation and survival of the U87 human glioblastoma cell line

    SciTech Connect (OSTI)

    Poch, Enric [Departamento de Quimica, Bioquimica y Biologia Molecular, Universidad Cardenal Herrera-CEU, Valencia (Spain); Minambres, Rebeca [Laboratorio de Patologia Celular, Centro de Investigacion Principe Felipe, Valencia (Spain); Mocholi, Enric [Departamento de Quimica, Bioquimica y Biologia Molecular, Universidad Cardenal Herrera-CEU, Valencia (Spain); Ivorra, Carmen [Departamento de Quimica, Bioquimica y Biologia Molecular, Universidad Cardenal Herrera-CEU, Valencia (Spain); Perez-Arago, Amparo [Laboratorio de Patologia Celular, Centro de Investigacion Principe Felipe, Valencia (Spain); Guerri, Consuelo [Laboratorio de Patologia Celular, Centro de Investigacion Principe Felipe, Valencia (Spain); Perez-Roger, Ignacio [Departamento de Quimica, Bioquimica y Biologia Molecular, Universidad Cardenal Herrera-CEU, Valencia (Spain)]. E-mail: iperez@uch.ceu.es; Guasch, Rosa M. [Laboratorio de Patologia Celular, Centro de Investigacion Principe Felipe, Valencia (Spain)]. E-mail: guasch@cipf.es

    2007-02-15

    Rho GTPases are important regulators of actin cytoskeleton, but they are also involved in cell proliferation, transformation and oncogenesis. One of this proteins, RhoE, inhibits cell proliferation, however the mechanism that regulates this effect remains poorly understood. Therefore, we undertook the present study to determine the role of RhoE in the regulation of cell proliferation. For this purpose we generated an adenovirus system to overexpress RhoE in U87 glioblastoma cells. Our results show that RhoE disrupts actin cytoskeleton organization and inhibits U87 glioblastoma cell proliferation. Importantly, RhoE expressing cells show a reduction in Rb phosphorylation and in cyclin D1 expression. Furthermore, RhoE inhibits ERK activation following serum stimulation of quiescent cells. Based in these findings, we propose that RhoE inhibits ERK activation, thereby decreasing cyclin D1 expression and leading to a reduction in Rb inactivation, and that this mechanism is involved in the RhoE-induced cell growth inhibition. Moreover, we also demonstrate that RhoE induces apoptosis in U87 cells and also in colon carcinoma and melanoma cells. These results indicate that RhoE plays an important role in the regulation of cell proliferation and survival, and suggest that this protein may be considered as an oncosupressor since it is capable to induce apoptosis in several tumor cell lines.

  5. Designing an ultra low quiescent current buck switching regulator

    E-Print Network [OSTI]

    Gardner, John Underhill

    2008-01-01

    The new buck regulator proposed in this thesis was designed to operate with only a few micro-amps of supply current during no load output conditions, while maintaining low output voltage ripple. The regulator also has high ...

  6. Transcriptional Regulation of Pregnane X Receptor by Protein Arginine Methyltransferase 

    E-Print Network [OSTI]

    Xie, Ying

    2011-08-08

    histone modifications such as the acetylation of histone H4. These modifications are believed to be important for transcriptional and epigenetic regulation of gene expression. We hypothesize that PRMT1 plays a role in the epigenetic changes regulated...

  7. Governing the gap: Forging safe science through relational regulation

    E-Print Network [OSTI]

    Huising, Ruthanne

    Designed to close the ubiquitous gap between law on the books and law in action, management systems locate the standard setting and implementation of regulation within the regulated organization itself. Despite efforts to ...

  8. A Dynamic Hobbesian Approach to Aggression and Self-Regulation

    E-Print Network [OSTI]

    Geldhof, Gordon John

    2008-06-18

    solid theoretical framework for examining self-regulation, aggression, and resource control. This paradigm encourages researchers to consider the functions of aggression (reactive vs. instrumental) and forms of self-regulation (active vs. automatic...

  9. Regulation Indentifier Number Title/Subject/Purpose Rule Type

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of a specific rule, please see the Federal Register at https:www.regulations.gov. Regulation Indentifier Number TitleSubjectPurpose Rule Type Status 1991-AB82 Acquisition...

  10. UNIVERSITY OF SOUTH FLORIDA STATEMENT ON EXPORT CONTROL REGULATIONS

    E-Print Network [OSTI]

    Arslan, Hüseyin

    **DRAFT** UNIVERSITY OF SOUTH FLORIDA STATEMENT ON EXPORT CONTROL REGULATIONS A. Export Control the export of certain technologies for reasons of national security or for the protection of trade (ITAR); the Department of Commerce through its Export Administration Regulations (EAR

  11. Transcriptional regulation and chromatin remodeling mechanisms at PHO5 

    E-Print Network [OSTI]

    Carvin, Christopher Dumas

    2005-08-29

    Regulation of gene expression is vital for proper growth and prevention of disease states. In eukaryotes this regulation occurs in the context of chromatin which creates an inherent barrier for the binding of trans-acting ...

  12. Effortful control and spontaneous regulation of emotional behavior in children 

    E-Print Network [OSTI]

    Kieras, Jessica E

    2013-02-22

    The development of Effortful Control and Regulation of Emotion in children was examined in this study. Of particular interest was the relationship between self-regulation and responses to social norms. Participants were between ages three and five...

  13. Energy storage for frequency regulation on the electric grid

    E-Print Network [OSTI]

    Leitermann, Olivia

    2012-01-01

    Ancillary services such as frequency regulation are required for reliable operation of the electric grid. Currently, the same traditional thermal generators that supply bulk power also perform nearly all frequency regulation. ...

  14. Petroleum Conveyance: The Petroleum-Spirit (Conveyance) Regulations 1953 

    E-Print Network [OSTI]

    Her Majesty's Stationary Office

    1953-09-09

    The Petroleum-Spirit (Conveyance) Regulations, 1939, do not make special provision for tank wagons and tank trailers designed to be filled through a filling opening in a manhole and without a fixed filling pipe. Regulations ...

  15. Improving Traffic Congestion By Regulating Employee Travel: A Phoenix Survey

    E-Print Network [OSTI]

    Burns, Elizabeth K.

    1991-01-01

    suburbancharacter. The City of Phoenix’s UrbanVillage PlanBy Regulating Employee Travel: A Phoenix Survey Elizabeth K.by Regulating Employee Travel: A Phoenix Survey Elizabeth K.

  16. From Biophysics to Evolutionary Genetics: Statistical Aspects of Gene Regulation

    E-Print Network [OSTI]

    Lässig, Michael

    , that it is not the only mode of gene interactions. Especially in eukaryotes, additional regulation mechanims involving

  17. The London Gas Undertakings (Regulations) Act 1939 (Modification) Order 1965 

    E-Print Network [OSTI]

    Lee, Fred

    1965-01-01

    STATUTORY INSTRUMENTS 1965 No. 1560 LONDON GOVERNMENT The London Gas Undertakings (Regulations) Act 1939 (Modification) Order 1965

  18. QB1 - Stochastic Gene Regulation Munsky, Brian [Los Alamos National

    Office of Scientific and Technical Information (OSTI)

    FACILITIES; FLUCTUATIONS; GENE REGULATION; KINETICS; QUANTIZATION; SIMULATION; SWITCHES Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present...

  19. The relationship between regulation and competition policy for network utilities

    E-Print Network [OSTI]

    Newbery, David

    regulation is powerful. The US evidence suggests that regulation evolves in response to pressures from special interest groups. On this view regulators are invariably captured, and use their regulatory powers to redistribute rents rather than to drive down... prospect of competition in supply, well- head gas prices were regulated, creating huge inefficiencies after the oil price rises of the 1970s. The subsequent collapse of the market as long-term contracts were signed at the peak of the oil price boom...

  20. A Utility Regulator's Guide to Data Access for Commercial Building...

    Broader source: Energy.gov (indexed) [DOE]

    benchmarking. A Utility Regulator's Guide to Data Access for Commercial Building Energy Performance Benchmarking More Documents & Publications Better Buildings Challenge...

  1. The Coal and Other Mines (Mechanics and Electricians) Regulations 1965 

    E-Print Network [OSTI]

    Lee, Fred

    1965-01-01

    STATUTORY INSTRUMENTS 1965 No. 1559 MINES AND QUARRIES The Coal and Other Mines (Mechanics and Electricians) Regulations 1965

  2. CAPTURE OR CONTRACT?: THE EARLY YEARS OF ELECTRIC UTILITY REGULATION

    E-Print Network [OSTI]

    Lyon, Thomas P.

    (forthcoming) study the historical origins of governance institutions for natural gas and water, respectively prices rose in states that adopted state regulation before 1917, suggesting that regulators were Friedland later found that the paper had underestimated the impact of regulation on prices by a factor

  3. Control of Wind Turbines for Power Regulation and

    E-Print Network [OSTI]

    Control of Wind Turbines for Power Regulation and Load Reduction Juan Jose Garcia Quirante Kongens@imm.dtu.dk www.imm.dtu.dk #12;#12;#12;Abstract This thesis describes the design of controllers for power regulation and load reduction and their ensemble in a variable-speed wind turbine. The power regulation

  4. Computational Molecular Biology of Genome Expression and Regulation

    E-Print Network [OSTI]

    Computational Molecular Biology of Genome Expression and Regulation Michael Q. Zhang, Ph.D. Cold in genome expression and regulation network in which better modern statistical and machine learning referring to the cellular processes that lead to pro- tein production, is controlled and regulated

  5. Environmental Regulation in Oligopoly Markets: A Study of Electricity Restructuring

    E-Print Network [OSTI]

    California at Berkeley. University of

    PWP-088 Environmental Regulation in Oligopoly Markets: A Study of Electricity Restructuring Erin T Regulation (POWER). POWER is a program of the University of California Energy Institute, a multicampus Regulation in Oligopoly Markets: A Study of Electricity Restructuring Erin T. Mansur April 6, 2004 Abstract

  6. Emotion Regulation Choice: A Conceptual Framework and Supporting Evidence

    E-Print Network [OSTI]

    Gross, James J.

    Emotion Regulation Choice: A Conceptual Framework and Supporting Evidence Gal Sheppes Tel Aviv that remains virtually unexplored is that of emotion regulation. This is surprising given that healthy adaptation requires flexibly choosing between regulation strategies in a manner that is responsive

  7. Supply Regulation Techniques for Phase-Locked Loops

    E-Print Network [OSTI]

    Palermo, Sam

    Supply Regulation Techniques for Phase-Locked Loops Vivekananth Gurumoorthy and Samuel Palermo-- Phase-locked loops (PLLs) which employ voltage regulators for low supply-noise sensitivity often rely. This paper compares various supply regulation techniques on the basis of their ability to reject noise from

  8. Regulation and controlled synchronization for complex dynamical systems

    E-Print Network [OSTI]

    Eindhoven, Technische Universiteit

    Regulation and controlled synchronization for complex dynamical systems H.J.C. Huijberts #3; H of controlled synchronization as a regulator problem. In controlled synchronization one is given autonomous of the problem where the standard solvability assumptions for the regulator problem are not met turn out to have

  9. Density regulation in annual plant communities under variable resource levels

    E-Print Network [OSTI]

    Novoplansky, Ariel

    Density regulation in annual plant communities under variable resource levels Hagit Shilo. E. and Turkington, R. 2005. Density regulation in annual plant communities under variable resource levels. Á/ Oikos 108: 241Á/252. Density regulation is assumed to be common, but is very rarely tested

  10. hal-00154748,version1-14Jun2007 Attenuation Regulation

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    hal-00154748,version1-14Jun2007 Attenuation Regulation as a Term Rewriting System Eugene Asarin1,lyubetsk@iitp.ru Abstract The classical attenuation regulation of gene expression in bac- teria is considered. We propose a probabilistic term rewriting system modeling the whole process of such a regulation. 1 Introduction Modeling

  11. Is My Operation Affected By This New Regulation?

    E-Print Network [OSTI]

    Bolding, M. Chad

    Is My Operation Affected By This New Regulation? This provision applies to all livestock operations that feed ruminants. The regulation applies to "establishments and individuals that are responsible operations, are subject to the regulation. ! Examples include dairies, cattle feedlots, calf and lamb raising

  12. Metabolic Regulation and Anticancer Drug Resistance in the Yeast

    E-Print Network [OSTI]

    Metabolic Regulation and Anticancer Drug Resistance in the Yeast Saccharomyces cerevisiae Mattias-91-576-8059-4 © 2014 Mattias Carlsson, Uppsala Print: SLU Service/Repro, Uppsala 2014 #12;Metabolic Regulation regulation by the yeast Rph1p protein, a zinc finger transcription factor which also contains a jmjC domain

  13. Frequency Regulation from Flexible Loads: Potential, Economics, and Implementation

    E-Print Network [OSTI]

    Sanandaji, Borhan M.

    Frequency Regulation from Flexible Loads: Potential, Economics, and Implementation He Hao1,, Borhan potential for providing regulation reserve to the grid. This paper aims to provide a foundation for a practical method of enabling TCLs to provide regulation service. We study the economic, regulatory

  14. Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin Biosynthesis

    E-Print Network [OSTI]

    Ismagilov, Rustem F.

    Article Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin Biosynthesis Graphical Abstract Highlights d Gut microbes regulate levels of 5-HT in the colon and blood d Spore-forming bacteria.cell.2015.02.047 #12;Article Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin

  15. NOTICE OF PROPOSED REGULATION AMENDMENT Date: August 18, 2005

    E-Print Network [OSTI]

    Wu, Shin-Tson

    and photovoltaic modules. The regulation provides updated information to the public to get details regarding. THE PERSON TO BE CONTACTED REGARDING THE PROPOSED REGULATION IS: Regulations Administrator 4000 Central as photovoltaic (herein called "PV") modules. The procedures and requirements for participating in the program

  16. NOTICE OF PROPOSED REGULATION AMENDMENT Date: August 18, 2005

    E-Print Network [OSTI]

    Wu, Shin-Tson

    THE PROPOSED REGULATION IS: Regulations Administrator 4000 Central Florida Blvd. Millican Hall, Suite 360 TEXT OF THE PROPOSED REGULATION AMENDMENT: 6C7-8.005 SolarThermal and Photovoltaic Domestic Water and Installation of Solar Thermal Systems" (May 2005) or FSEC Standard 203-05, "Procedures for Photovoltaic System

  17. Legal Authority 1 Export Administration Regulations EARB 298 -October 2001

    E-Print Network [OSTI]

    Bernstein, Daniel

    Legal Authority 1 Export Administration Regulations EARB 298 - October 2001 Part I PRINCIPAL STATUTORY AUTHORITY FOR THE EXPORT ADMINISTRATION REGULATIONS #12;Legal Authority 2 1 The Act expired Presidential notices, continued in effect the provisions of the Export Administration Regulations. The Act

  18. Regulation Summary Export Controls are Overseen by Three Federal Agencies

    E-Print Network [OSTI]

    Bordenstein, Seth

    Regulation Summary Export Controls are Overseen by Three Federal Agencies 29May2014 Federal Agency Name of Regulations/Division Exports Covered Description Department of State International Traffic on the USML requiring a license Department of Commerce Export Administration Regulations (EAR) 15 CFR § 730

  19. General Information Part 730page 1 Export Administration Regulations

    E-Print Network [OSTI]

    Bernstein, Daniel

    General Information Part 730­page 1 Export Administration Regulations §730.1 WHAT THESE REGULATIONS COVER In this part, references to the Export Administra- tion Regulations (EAR) are references to 15 CFR of Export Administration (BXA) under laws relating to the control of certain exports, reexports

  20. NOTICE OF NEW REGULATION Date: September 16, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    Conduct Review Process; Sanctions; UCF-5.013 Appeals SUMMARY OF REGULATION: The purpose of this new regulation is to outline the review process for student organization conduct violations and the sanctions OF THE PROPOSED REGULATION: UCF-5.013 Organization Conduct Review Process; Sanctions; Appeals (1) Violation

  1. NOTICE OF REGULATION REPEAL Date: July 1, 2010

    E-Print Network [OSTI]

    Wu, Shin-Tson

    and Photovoltaic System UCF-8.005 Standards and Certification SUMMARY OF REGULATION REPEAL: This regulation OF THE PROPOSED REGULATION REPEAL: UCF-8.005 Solar Thermal and Photovoltaic System Standards and Certification Thermal Systems" (May 2005) or FSEC Standard 203-05, "Procedures for Photovoltaic System Design Review

  2. Mapping Regulations to Industry-Specific Taxonomies Chin Pang Cheng

    E-Print Network [OSTI]

    Stanford University

    for regulations. In this paper, we present a systematic approach to mapping regulations to industryMapping Regulations to Industry-Specific Taxonomies Chin Pang Cheng Stanford University Dept For each industry, there exist many taxonomies that are intended for various applications. There are also

  3. NOTICE OF PROPOSED REGULATION AMENDMENT Date: April 15, 2009

    E-Print Network [OSTI]

    Wu, Shin-Tson

    .: Solar Thermal Collector and PV Module Certification UCF-8.003 SUMMARY OF REGULATION AMENDMENT-mail: regulations@mail.ucf.edu FULL TEXT OF THE PROPOSED REGULATION AMENDMENT: UCF6C7-8.003 Solar Thermal Collector of a solar thermal collector that has met the requirements of FSEC Standard 101-095 and for which

  4. Up-regulation of K{sub ir}2.1 by ER stress facilitates cell death of brain capillary endothelial cells

    SciTech Connect (OSTI)

    Kito, Hiroaki; Yamazaki, Daiju; Department of Biological Chemistry, Kyoto University, Graduate School of Pharmaceutical Sciences, Kyoto; Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Nagoya ; Ohya, Susumu; Yamamura, Hisao; Asai, Kiyofumi; Imaizumi, Yuji

    2011-07-29

    Highlights: {yields} We found that application of endoplasmic reticulum (ER) stress with tunicamycin to brain capillary endothelial cells (BCECs) induced cell death. {yields} The ER stress facilitated the expression of inward rectifier K{sup +} channel (K{sub ir}2.1) and induced sustained membrane hyperpolarization. {yields} The membrane hyperpolarization induced sustained Ca{sup 2+} entry through voltage-independent nonspecific cation channels and consequently facilitated cell death. {yields} The K{sub ir}2.1 up-regulation by ER stress is, at least in part, responsible for cell death of BCECs under pathological conditions. -- Abstract: Brain capillary endothelial cells (BCECs) form blood brain barrier (BBB) to maintain brain homeostasis. Cell turnover of BCECs by the balance of cell proliferation and cell death is critical for maintaining the integrity of BBB. Here we found that stimuli with tunicamycin, endoplasmic reticulum (ER) stress inducer, up-regulated inward rectifier K{sup +} channel (K{sub ir}2.1) and facilitated cell death in t-BBEC117, a cell line derived from bovine BCECs. The activation of K{sub ir} channels contributed to the establishment of deeply negative resting membrane potential in t-BBEC117. The deep resting membrane potential increased the resting intracellular Ca{sup 2+} concentration due to Ca{sup 2+} influx through non-selective cation channels and thereby partly but significantly regulated cell death in t-BBEC117. The present results suggest that the up-regulation of K{sub ir}2.1 is, at least in part, responsible for cell death/cell turnover of BCECs induced by a variety of cellular stresses, particularly ER stress, under pathological conditions.

  5. PES1 regulates sensitivity of colorectal cancer cells to anticancer drugs

    SciTech Connect (OSTI)

    Xie, Wei; Qu, Like; Meng, Lin; Liu, Caiyun; Wu, Jian; Shou, Chengchao

    2013-02-15

    Highlights: ? PES1 was overexpressed in diverse cancer cell lines. ? PES1-ablation enhances DNA damage response by decreasing DNA repair. ? PES1-ablation increases the sensitivity of HCT116 cells to chemotherapeutic agents. ? PES1-ablation is associated with diminished nuclear entry of RAD51. -- Abstract: PES1 (also known as Pescadillo), a nucleolar protein, was involved in biogenesis of ribosomal RNA. Up-regulation of PES1 has been documented in some human cancers, indicating that PES1 may play some crucial roles in tumorigenesis. In our previous study, it was found that silencing of PES1 resulted in decreased proliferation of colorectal cancer cells. We also noticed that depletion of PES1 altered expression profiles of diverse genes. In the present study, we validated the expression changes of a subset of genotoxic stress-related genes in PES1-silenced HCT116 cells by quantitative RT-PCR. The steady and etoposide-induced phosphorylated H2AX (?-H2AX) were higher in PES1-silenced cells than in control cells. Besides, etoposide-induced ?-H2AX persisted longer in PES1-silenced cells after removing the etoposide. Next, results of comet assay revealed decreased DNA repair after PES1-ablation. PES1-ablated cells were more sensitive to chemotherapeutic agents, which could be reversed by reconstitution with exogenous PES1. Furthermore, deletion of PES1 diminished steady and DNA damage-induced levels of nuclear RAD51. Our results uncover a potential role of PES1 in chemoresistance by regulating DNA damage response in colorectal cancer cells.

  6. Evolving Role of the Power Sector Regulator: A Clean Energy Regulators Initiative Report

    SciTech Connect (OSTI)

    Zinaman, O.; Miller, M.; Bazilian, M.

    2014-04-01

    This paper seeks to briefly characterize the evolving role of power sector regulation. Given current global dynamics, regulation of the power sector is undergoing dramatic changes. This transformation is being driven by various factors including technological advances and cost reductions in renewable energy, energy efficiency, and demand management; increasing air pollution and climate change concerns; and persistent pressure for ensuring sustainable economic development and increased access to energy services by the poor. These issues add to the already complex task of power sector regulation, of which the fundamental remit remains to objectively and transparently ensure least-cost service delivery at high quality. While no single regulatory task is trivial to undertake, it is the prioritization and harmonization of a multitude of objectives that exemplifies the essential challenge of power sector regulation. Evolving regulatory roles can be understood through the concept of existing objectives and an additional layer of emerging objectives. Following this categorization, we describe seven existing objectives of power sector regulators and nine emerging objectives, highlighting key challenges and outlining interdependencies. This essay serves as a preliminary installment in the Clean Energy Regulatory Initiative (CERI) series, and aims to lay the groundwork for subsequent reports and case studies that will explore these topics in more depth.

  7. INPP4B-mediated tumor resistance is associated with modulation of glucose metabolism via hexokinase 2 regulation in laryngeal cancer cells

    SciTech Connect (OSTI)

    Min, Joong Won; Kim, Kwang Il; Kim, Hyun-Ah; Kim, Eun-Kyu; Noh, Woo Chul; Jeon, Hong Bae; Cho, Dong-Hyung; Oh, Jeong Su; Park, In-Chul; Hwang, Sang-Gu; Kim, Jae-Sung

    2013-10-11

    Highlights: •HIF-1?-regulated INPP4B enhances glycolysis. •INPP4B regulates aerobic glycolysis by inducing HK2 via Akt-mTOR pathway. •Blockage of INPP4B and HK2 sensitizes radioresistant laryngeal cancer cells to radiation and anticancer drug. •INPP4B is associated with HK2 in human laryngeal cancer tissues. -- Abstract: Inositol polyphosphate 4-phosphatase type II (INPP4B) was recently identified as a tumor resistance factor in laryngeal cancer cells. Herein, we show that INPP4B-mediated resistance is associated with increased glycolytic phenotype. INPP4B expression was induced by hypoxia and irradiation. Intriguingly, overexpression of INPP4B enhanced aerobic glycolysis. Of the glycolysis-regulatory genes, hexokinase 2 (HK2) was mainly regulated by INPP4B and this regulation was mediated through the Akt-mTOR pathway. Notably, codepletion of INPP4B and HK2 markedly sensitized radioresistant laryngeal cancer cells to irradiation or anticancer drug. Moreover, INPP4B was significantly associated with HK2 in human laryngeal cancer tissues. Therefore, these results suggest that INPP4B modulates aerobic glycolysis via HK2 regulation in radioresistant laryngeal cancer cells.

  8. Omentin inhibits TNF-{alpha}-induced expression of adhesion molecules in endothelial cells via ERK/NF-{kappa}B pathway

    SciTech Connect (OSTI)

    Zhong, Xia, E-mail: zhongxia1977@126.com [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China); Li, Xiaonan; Liu, Fuli; Tan, Hui [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China); Shang, Deya, E-mail: wenhuashenghuo1@163.com [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)

    2012-08-24

    Highlights: Black-Right-Pointing-Pointer Omentin inhibited TNF-{alpha}-induced adhesion of THP-1 cells to HUVECs. Black-Right-Pointing-Pointer Omentin reduces expression of ICAM-1 and VCAM-1 induced by TNF-{alpha} in HUVECs. Black-Right-Pointing-Pointer Omentin inhibits TNF-{alpha}-induced ERK and NF-{kappa}B activation in HUVECs. Black-Right-Pointing-Pointer Omentin supreeses TNF-{alpha}-induced expression of ICAM-1 and VCAM-1 via ERK/NF-{kappa}B pathway. -- Abstract: In the present study, we investigated whether omentin affected the expression of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in tumor necrosis factor-{alpha} (TNF-{alpha}) induced human umbilical vein endothelial cells (HUVECs). Our data showed that omentin decreased TNF-{alpha}-induced expression of ICAM-1 and VCAM-1 in HUVECs. In addition, omentin inhibited TNF-{alpha}-induced adhesion of THP-1 cells to HUVECs. Further, we found that omentin inhibited TNF-{alpha}-activated signal pathway of nuclear factor-{kappa}B (NF-{kappa}B) by preventing NF-{kappa}B inhibitory protein (I{kappa}B{alpha}) degradation and NF-{kappa}B/DNA binding activity. Omentin pretreatment significantly inhibited TNF-{alpha}-induced ERK activity and ERK phosphorylation in HUVECs. Pretreatment with PD98059 suppressed TNF-{alpha}-induced NF-{kappa}B activity. Omentin, NF-kB inhibitor (BAY11-7082) and ERK inhibitor (PD98059) reduced the up-regulation of ICAM-1 and VCAM-1 induced by TNF-{alpha}. These results suggest that omentin may inhibit TNF-{alpha}-induced expression of adhesion molecules in endothelial cells via blocking ERK/NF-{kappa}B pathway.

  9. Nickel-regulated heart rate variability: The roles of oxidative stress and inflammation

    SciTech Connect (OSTI)

    Chuang, Hsiao-Chi; Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan ; Hsueh, Tzu-Wei; Chang, Chuen-Chau; Hwang, Jing-Shiang; Chuang, Kai-Jen; School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan ; Yan, Yuan-Horng; Department of Medical Research, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi City, Taiwan ; Cheng, Tsun-Jen; Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan

    2013-01-15

    Heart rate variability (HRV) has been reported to be a putative marker of cardiac autonomic imbalance caused by exposure to ambient particulate matter (PM). Our objective in this study was to determine the effects on HRV from exposure to nickel, an important chemical component of ambient PM that results in oxidative stress and inflammation. HRV data were collected for 72 h before lung exposure (baseline) and 72 h after intratracheal exposure (response) to nickel sulphate (NiSO{sub 4}; 526 ?g) in Wistar Kyoto (WKY) and spontaneously hypertensive (SH) rats. The antioxidant N-acetyl-L-cysteine (NAC) and the anti-inflammatory celecoxib were intraperitoneally injected to examine post-exposure oxidative and inflammatory responses. Self-controlled experiments examined the effects of NiSO{sub 4} exposure on average normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN) and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD); the resulting data were sequentially analysed using the generalised estimating equation model. HRV effects on NiSO{sub 4}-exposed SH rats were greater than those on NiSO{sub 4}-exposed WKY rats. After adjusted the HRV responses in the WKY rats as control, ANN and LnRMSSD were found to be quadratically increased over 72 h after exposure to NiSO{sub 4}. Both NAC and celecoxib mitigated the NiSO{sub 4}-induced alterations in HRV during the exposure period. The results suggest that concurrent Ni-induced oxidative stress and inflammatory responses play important roles in regulating HRV. These findings help bridge the gap between epidemiological and clinical studies on the plausible mechanisms of the cardiovascular consequences induced by chemical components in ambient PM. -- Highlights: ? To determine the effects on HRV from exposure to nickel. ? ANN and LnRMSSD were found to be quadratically increased after exposure to Ni. ? NAC and celecoxib mitigated the Ni-induced alterations in HRV. ? Ni-induced oxidative stress and inflammation play the roles in regulating HRV.

  10. Aromatic hydrocarbon receptor inhibits lysophosphatidic acid-induced vascular endothelial growth factor-A expression in PC-3 prostate cancer cells

    SciTech Connect (OSTI)

    Wu, Pei-Yi; Lin, Yueh-Chien; Lan, Shun-Yan [Institute of Zoology, National Taiwan University, Taipei, Taiwan (China)] [Institute of Zoology, National Taiwan University, Taipei, Taiwan (China); Huang, Yuan-Li [Department of Biotechnology, Asia University, Taichung, Taiwan (China)] [Department of Biotechnology, Asia University, Taichung, Taiwan (China); Lee, Hsinyu, E-mail: hsinyu@ntu.edu.tw [Institute of Zoology, National Taiwan University, Taipei, Taiwan (China) [Institute of Zoology, National Taiwan University, Taipei, Taiwan (China); Department of Life Science, National Taiwan University, Taipei, Taiwan (China)

    2013-08-02

    Highlights: •LPA-induced VEGF-A expression was regulated by HIF-1? and ARNT. •PI3K mediated LPA-induced VEGF-A expression. •AHR signaling inhibited LPA-induced VEGF-A expression in PC-3 cells. -- Abstract: Lysophosphatidic acid (LPA) is a lipid growth factor with multiple biological functions and has been shown to stimulate cancer cell secretion of vascular endothelial growth factor-A (VEGF-A) and trigger angiogenesis. Hypoxia-inducible factor-1 (HIF-1), a heterodimer consisting of HIF-1? and HIF-1? (also known as aromatic hydrocarbon receptor nuclear translocator (ARNT)) subunits, is an important regulator of angiogenesis in prostate cancer (PC) through the enhancement of VEGF-A expression. In this study, we first confirmed the ability of LPA to induce VEGF-A expression in PC-3 cells and then validated that LPA-induced VEGF-A expression was regulated by HIF-1? and ARNT through phosphatidylinositol 3-kinase activation. Aromatic hydrocarbon receptor (AHR), a receptor for dioxin-like compounds, functions as a transcription factor through dimerization with ARNT and was found to inhibit prostate carcinogenesis and vanadate-induced VEGF-A production. Since ARNT is a common dimerization partner of AHR and HIF-1?, we hypothesized that AHR might suppress LPA-induced VEGF-A expression in PC-3 cells by competing with HIF-1? for ARNT. Here we demonstrated that overexpression and ligand activation of AHR inhibited HIF-1-mediated VEGF-A induction by LPA treatment of PC-3 cells. In conclusion, our results suggested that AHR activation may inhibit LPA-induced VEGF-A expression in PC-3 cells by attenuating HIF-1? signaling, and subsequently, suppressing angiogenesis and metastasis of PC. These results suggested that AHR presents a potential therapeutic target for the prevention of PC metastasis.

  11. Metformin induces differentiation in acute promyelocytic leukemia by activating the MEK/ERK signaling pathway

    SciTech Connect (OSTI)

    Huai, Lei; Wang, Cuicui; Zhang, Cuiping; Li, Qihui; Chen, Yirui; Jia, Yujiao; Li, Yan; Xing, Haiyan; Tian, Zheng; Rao, Qing; Wang, Min [State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 (China)] [State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 (China); Wang, Jianxiang, E-mail: wangjx@ihcams.ac.cn [State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 (China)] [State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 (China)

    2012-06-08

    Highlights: Black-Right-Pointing-Pointer Metformin induces differentiation in NB4 and primary APL cells. Black-Right-Pointing-Pointer Metformin induces activation of the MEK/ERK signaling pathway in APL cells. Black-Right-Pointing-Pointer Metformin synergizes with ATRA to trigger maturation of NB4 and primary APL cells. Black-Right-Pointing-Pointer Metformin induces the relocalization and degradation of the PML-RAR{alpha} fusion protein. Black-Right-Pointing-Pointer The study may be applicable for new differentiation therapy in cancer treatment. -- Abstract: Recent studies have shown that metformin, a widely used antidiabetic agent, may reduce the risk of cancer development. In this study, we investigated the antitumoral effect of metformin on both acute myeloid leukemia (AML) and acute promyelocytic leukemia (APL) cells. Metformin induced apoptosis with partial differentiation in an APL cell line, NB4, but only displayed a proapoptotic effect on several non-M3 AML cell lines. Further analysis revealed that a strong synergistic effect existed between metformin and all-trans retinoic acid (ATRA) during APL cell maturation and that metformin induced the hyperphosphorylation of extracellular signal-regulated kinase (ERK) in APL cells. U0126, a specific MEK/ERK activation inhibitor, abrogated metformin-induced differentiation. Finally, we found that metformin induced the degradation of the oncoproteins PML-RAR{alpha} and c-Myc and activated caspase-3. In conclusion, these results suggest that metformin treatment may contribute to the enhancement of ATRA-induced differentiation in APL, which may deepen the understanding of APL maturation and thus provide insight for new therapy strategies.

  12. Superthreshold Behavior of Ultrasound-Induced Lung

    E-Print Network [OSTI]

    Illinois at Urbana-Champaign, University of

    Superthreshold Behavior of Ultrasound-Induced Lung Hemorrhage in Adult Rats Role of Pulse and reevaluate the ultra- sound-induced lung hemorrhage findings of a previous 5 × 3 factorial design study, 250, and 500 Hz) and exposure duration (ED; 5, 10, and 20 s) on ultrasound-induced lung hemorrhage

  13. Regulation of human hepatocellular carcinoma cells by Spred2 and correlative studies on its mechanism

    SciTech Connect (OSTI)

    Ma, Xiao-Ni [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Liu, Xiao-Yun [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China) [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Center for Disease Control and Prevention, Lanzhou Military Command, Lanzhou 730020 (China); Yang, Yue-Feng [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Xiao, Feng-Jun [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Li, Qing-Fang [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Yan, Jun [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Zhang, Qun-Wei; Wang, Li-Sheng [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China); Li, Xue-Yan, E-mail: llglixueyan@163.com [Lanzhou University of Technology, Lanzhou 730050 (China)] [Lanzhou University of Technology, Lanzhou 730050 (China); Wang, Hua, E-mail: wanghua@bmi.ac.cn [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)] [Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)

    2011-07-15

    Highlights: {yields} Hepatocellular carcinoma is inhibited by Spred2 through as yet unclear mechanisms. {yields} We studied the overexpression of Spred2 in cell line and murine tumor models of HCC. {yields} Spred2 inhibited cell proliferation and migration via attenuating ERK signaling. {yields} Spred2 overexpression induced apoptosis via caspase-3 and downregulated Mcl-1. {yields} A Spred2 knockdown markedly induced tumor growth in vivo. -- Abstract: Members of the Spred gene family are negative regulators of the Ras/Raf-1/ERK pathway, which has been associated with several features of the tumor malignancy. However, the effect of Spred genes on hepatocellular carcinoma (HCC) remains uninvestigated. In the present work, we analyzed the in vitro and in vivo effects of Spred2 expression on the hepatic carcinoma cell line, SMMC-7721. In addition to attenuated ERK activation, which inhibited the proliferation and migration of unstimulated and HGF-stimulated SMMC-7721 cells. Adenovirus-mediated Spred2 overexpression induced the activation of caspase-3 and apoptosis, as well as reduced the expression level of Mcl-1. Most importantly, the knockdown of Spred2 markedly enhanced tumor growth in vivo. In conclusion, these results suggest that Spred2 could qualify as a potential therapeutic target in HCC.

  14. The future of financial markets and regulation: What Strategy for Europe? The Future of Financial Markets and Regulation

    E-Print Network [OSTI]

    Boyer, Edmond

    The future of financial markets and regulation: What Strategy for Europe? 1 The Future of Financial Markets and Regulation: What Strategy for Europe? Jean-Baptiste Gossé1 Dominique Plihon2 Abstract This article provides insight into the future of financial markets and regulation in order to define what would

  15. AUTOMATIC EMOTION REGULATION 1 Mauss, I. B., Bunge, S. A., & Gross, J. J. (2007). Automatic emotion regulation. Social and

    E-Print Network [OSTI]

    Gross, James J.

    2007-01-01

    AUTOMATIC EMOTION REGULATION 1 Mauss, I. B., Bunge, S. A., & Gross, J. J. (2007). Automatic emotion://www.blackwell-compass.com/subject/socialpsychology/ Automatic Emotion Regulation Iris B. Mauss1 , Silvia A. Bunge2 , & James J. Gross3 1 University of Denver 2 University of California, Berkeley 3 Stanford University #12;AUTOMATIC EMOTION REGULATION 2 Abstract How do

  16. Regulation and Property Values in the United States: The High Cost of Monopoly

    E-Print Network [OSTI]

    Quigley, John M.

    2007-01-01

    F. T. Ventre. 1971. Local regulation of building: Agencies,local government land use regulation. Cambridge, MA: LincolnManhattan so expensive? Regulation and the rise in housing

  17. Beyond control - The Uncertainties and Diverging Images of Swedish Chemicals Regulation

    E-Print Network [OSTI]

    Haikola, Simon

    2015-01-01

    and European chemicals regulation. Journal of EuropeanSwedish chemicals regulation: An overview and analysis. Riskprinciple in chemical regulation: A constructive critique.

  18. The Regulation of E-learning: New National and International Policy Perspectives

    E-Print Network [OSTI]

    Harley, Diane; Lawrence, Shannon

    2007-01-01

    Colleges (WASC) THE REGULATION OF E-LEARNING: NEW NATIONALcshe.berkeley.edu/research/regulation/documents/dla_interim_and institutional norms. Regulation is generally driven by

  19. Energy Regulation, Roll Call Votes and Regional Resources: Evidence from Russia

    E-Print Network [OSTI]

    Grigoriadis, Theocharis N; Torgler, Benno

    2007-01-01

    Problems of the North 20. Regulation and Work organizationV. "Reform and Regulation of Natural Monopolies." (InEnergy Regulation, Roll Call Votes and Regional Resources:

  20. Shaping the terms of competition : environmental regulation and corporate strategies to reduce diesel vehicle emissions

    E-Print Network [OSTI]

    Ng, Christine Bik-Kay, 1979-

    2006-01-01

    Environmental regulations are typically portrayed as an outside force stimulating development of environmental technologies in regulated industries. In reality, firms influence regulation by communicating their technological ...

  1. Genetic analysis of the regulation of TCH gene expression, Final Report

    SciTech Connect (OSTI)

    Braam, Janet

    2008-10-28

    The Arabidopsis TCH genes, originally isolated as a consequence of their upregulation in response to the mechanical stimulus of touch, are also upregulated by a variety of seemingly disparate environmental and hormonal stimuli. To gain insight into the complexities of TCH gene regulation, a number of approaches were taken. Regulatory elements responsible for regulation were identified and characteristics of the regulation were evaluated. Reporter genes were used to monitor expression localization and dynamics. Microarray analyses of genome-wide expression behavior indicated that touch-inducible gene expression is more widespread than generally appreciated. Identification of all touch-regulated genes shed light on the types of cellular processes that may be altered in response to mechanical stress perturbations. Expression of the TCH2 gene, also called CML24, encoding a calmodulin (CaM)-like (CML) protein, was evaluated. CML24 shares over 40% amino acid sequence identity with CaM, has 4 EF hands and undergoes a Ca2+-dependent change in migration rate through denaturing gel electrophoresis, indicating that CML24 binds Ca2+ and, as a consequence, undergoes conformational changes. CML24 expression occurs in all major organs and is induced from 2- to 15-fold in plants subjected to touch, darkness, heat, cold, hydrogen peroxide, abscisic acid (ABA) and indole-3-acetic acid. The putative CML24 regulatory region confers reporter expression at sites of predicted mechanical stress, in regions undergoing growth, in vascular tissues and various floral organs and in stomata, trichomes and hydathodes. CML24 underexpressing transgenics are resistant to ABA inhibition of germination and seedling growth, defective in long-day induction of flowering, and have enhanced tolerance to CoCl2, molybdic acid, ZnSO4 and MgCl2. These data present evidence that CML24 encodes a potential Ca2+ sensor that may function to enable responses to ABA, day length and presence of various salts. Further investigation of CML24 function and regulation led to the finding that CML24 has a critical role in nitric oxide regulation. Distinct tilling mutant alleles demonstrated that CML24 can act as a switch in the response to day length perception. Because of potential redundancy with the related CML23 gene, CML23 T-DNA insertion mutants were identified and characterized. Together, CML23 and CML24 impact the autonomous regulatory pathway of the transition to flowering. Nitric oxide levels are elevated in cml23/cml24 double mutants. Therefore, CML23 and CML24 are potential calcium sensors regulate nitric oxide accumulation. In collaboration with Drs. McCann and Carpita, fourier transform infrared spectroscopy (FTIR) was used to assess, verify and classify wall architectural changes that occur as a result of single XTH insertion mutations. Thirty-four homozygous mutant lines of Arabidopsis representing 21 members of the xyloglucan endotransglucosylase/hydrolase gene family provided a set of mutants to characterize. Kohonen networks classified cell wall architectures of xth mutant lines and previously characterized cell wall mutants. The xth mutants were found to have chemical changes in their cell walls not detectable as phenotypic growth and development changes, consistent with the existence of feed-back loops that modify wall composition in response to a life-long deficiency of a cell wall enzyme. To gain insight into the potential physiological relevance of the distinct members of the XTH family, GUS reporter fusion genes were constructed, and plants expressing these transgenes were characterized to reveal spatial and temporal patterns of expression. In addition, Genevestigator sources were mined for comprehensive and comparative XTH expression regulation analysis. These data revealed that the Arabidopsis XTHs are likely expressed in every developmental stage from seed germination through flowering. All organs showed XTH::GUS expression and most, if not all, are found to express multiple XTH::GUS genes suggesting that XTHs may contribute to morphogenesis at every d

  2. Porcine circovirus type 2 replication is impaired by inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway

    SciTech Connect (OSTI)

    Wei Li [Institute of Animal Husbandry and Veterinary Medicine, Beijing Municipal Academy of Agriculture and Forestry Sciences, No.9 Shuguang Garden Central Road, Haidian District, Beijing 100097 (China); Liu Jue [Institute of Animal Husbandry and Veterinary Medicine, Beijing Municipal Academy of Agriculture and Forestry Sciences, No.9 Shuguang Garden Central Road, Haidian District, Beijing 100097 (China)], E-mail: liujue@263.net

    2009-03-30

    Postweaning multisystemic wasting syndrome, which is primarily caused by porcine circovirus type 2 (PCV2), is an emerging and important swine disease. We have recently shown that PCV2 induces nuclear factor kappa B activation and its activation is required for active replication, but the other cellular factors involved in PCV2 replication are not well defined. The extracellular signal-regulated kinase (ERK) which served as an important component of cellular signal transduction pathways has been shown to regulate many viral infections. In this report, we show that PCV2 activates ERK1/2 in PCV2-infected PK15 cells dependent on viral replication. The PCV2-induced ERK1/2 leads to phosphorylation of the ternary complex factor Elk-1, which kinetically paralleled ERK1/2 activation. Inhibition of ERK activation with U0126, a specific MEK1/2 inhibitor, significantly reduced viral progeny release. Investigations into the mechanism of ERK1/2 regulation revealed that inhibition of ERK activation leads to decreased viral transcription and lower virus protein expression. These data indicate that the ERK signaling pathway is involved in PCV2 infection and beneficial to PCV2 replication in the cultured cells.

  3. Caveolin-2 regulation of the cell cycle in response to insulin in Hirc-B fibroblast cells

    SciTech Connect (OSTI)

    Kim, Sangmin [Department of Biochemistry, Division of Life Science, College of Natural Sciences, Graduate School of Applied Life Science, Gyeongsang National University, Jinju 660-701 (Korea, Republic of); Pak, Yunbae [Department of Biochemistry, Division of Life Science, College of Natural Sciences, Graduate School of Applied Life Science, Gyeongsang National University, Jinju 660-701 (Korea, Republic of)]. E-mail: ybpak@nongae.gsnu.ac.kr

    2005-04-29

    The regulatory function of caveolin-2 in cell cycle regulation by insulin was investigated in human insulin receptor-overexpressed rat 1 fibroblast (Hirc-B) cells. Insulin increased induction of the caveolin-2 gene in a time-dependent manner. Direct interaction between ERK and caveolin-2 was confirmed by immunoprecipitation and phosphorylated ERK increased the specific interaction in response to insulin. That insulin induced their nuclear co-localization over time was demonstrated by immunofluorescence microscopy. Insulin increased the S phase in the cell cycle by 6-fold. When recombinant caveolin-1 was transiently expressed, a decrease in the S phase was detected by flow-cytometry. The results indicate that the up-regulation of caveolin-2 in response to insulin activates the downstream signal cascades in the cell cycle, chiefly the increased phosphorylation of ERK, the nuclear translocation of phosphorylated ERK, and the subsequent activation of G0/G1 to S phase transition of the cell cycle. The results also suggest that DNA synthesis and the activation of the cell cycle by insulin are achieved concomitantly with an increase in the interaction between caveolin-2 and phosphorylated ERK, and the nuclear translocation of that complex. Taken together, we conclude that caveolin-2 positively regulates the insulin-induced cell cycle through activation of and direct interaction with ERK in Hirc-B cells.

  4. Self-regulating flow control device

    DOE Patents [OSTI]

    Humphreys, Duane A. (Pittsburgh, PA)

    1984-01-01

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  5. Environmental Regulation Impacts on Eastern Interconnection Performance

    SciTech Connect (OSTI)

    Markham, Penn N; Liu, Yilu; Young II, Marcus Aaron

    2013-07-01

    In the United States, recent environmental regulations will likely result in the removal of nearly 30 GW of oil and coal-fired generation from the power grid, mostly in the Eastern Interconnection (EI). The effects of this transition on voltage stability and transmission line flows have previously not been studied from a system-wide perspective. This report discusses the results of power flow studies designed to simulate the evolution of the EI over the next few years as traditional generation sources are replaced with environmentally friendlier ones such as natural gas and wind.

  6. Property:Regulations | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource HistoryPotentialRuralUtilityScalePVGeneration Jump to: navigation,Power TransferProjectType JumpRegulations

  7. Alaska ADEC Wetlands Regulation | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EAand DaltonSolarOpen5 - Applications Jump to:Wetlands Regulation Jump to:

  8. Policy and Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuels Data CenterFinancialInvesting inServices »About UsAbout theRegulations Policy

  9. SIRT1 inactivation induces inflammation through the dysregulation of autophagy in human THP-1 cells

    SciTech Connect (OSTI)

    Takeda-Watanabe, Ai; Kitada, Munehiro; Kanasaki, Keizo; Koya, Daisuke

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer SIRT1 inactivation decreases autophagy in THP-1 cell. Black-Right-Pointing-Pointer Inhibition of autophagy induces inflammation. Black-Right-Pointing-Pointer SIRT1 inactivation induces inflammation through NF-{kappa}B activation. Black-Right-Pointing-Pointer The p62/Sqstm1 accumulation by impairment of autophagy is related to NF-{kappa}B activation. Black-Right-Pointing-Pointer SIRT1 inactivation is involved in the activation of mTOR and decreased AMPK activation. -- Abstract: Inflammation plays a crucial role in atherosclerosis. Monocytes/macrophages are some of the cells involved in the inflammatory process in atherogenesis. Autophagy exerts a protective effect against cellular stresses like inflammation, and it is regulated by nutrient-sensing pathways. The nutrient-sensing pathway includes SIRT1, a NAD{sup +}-dependent histone deacetylase, which is implicated in the regulation of a variety of cellular processes including inflammation and autophagy. The mechanism through which the dysfunction of SIRT1 contributes to the regulation of inflammation in relation to autophagy in monocytes/macrophages is unclear. In the present study, we demonstrate that treatment with 2-[(2-Hydroxynaphthalen-1-ylmethylene)amino]-N-(1-phenethyl)benzamide (Sirtinol), a chemical inhibitor of SIRT1, induces the overexpression of inflammation-related genes such as tumor necrosis factor (TNF)-{alpha} and interleukin (IL)-6 through nuclear factor (NF)-{kappa}B signaling activation, which is associated with autophagy dysfunction, as shown through p62/Sqstm1 accumulation and decreased expression of light chain (LC) 3 II in THP-1 cells. The autophagy inhibitor, 3-methyladenine, also induces inflammation-related NF-{kappa}B activation. In p62/Sqstm1 knockdown cells, Sirtinol-induced inflammation through NF-{kappa}B activation is blocked. In addition, inhibition of SIRT1 is involved in the activation of the mammalian target of rapamycin (mTOR) pathway and is implicated in decreased 5 Prime -AMP activated kinase (AMPK) activation, leading to the impairment of autophagy. The mTOR inhibitor, rapamycin, abolishes Sirtinol-induced inflammation and NF-{kappa}B activation associated with p62/Sqstm1 accumulation. In summary, SIRT1 inactivation induces inflammation through NF-{kappa}B activation and dysregulates autophagy via nutrient-sensing pathways such as the mTOR and AMPK pathways, in THP-1 cells.

  10. Universidad Central de Venezuela Facultad de Ciencias

    E-Print Network [OSTI]

    Coto, Ernesto

    problemas más complejos como los de flujo en redes. Es recomendable que el lector tenga conocimientos 10. COMPONENTES FUERTEMENTE CONEXAS 22 11. TOUR HAMILTONIANO Y TSP 23 12. FLUJO EN REDES 23 13 que contienen algún tipo de información y las líneas o flechas son conexiones o relaciones entre estos

  11. El teatro de corte histórico en Venezuela

    E-Print Network [OSTI]

    Herrera, Carlos E.

    1988-04-01

    hecho de su tiempo a través de la utilización de una refe rencia histórica? ¿Cómo puede ser entendida la función de este tipo de teatro? Del tipo de aproximación o de separación que el dramaturgo establezca con las relaciones teatro/historia, se... histórico comporta una verosimilitud escénica dependiente del tipo de lenguaje utilizado, podrá proyectar ese hecho histórico- real o no-a un nivel de interpretación conveniente para promover un nuevo juicio crítico. Pero la historia como punto de...

  12. Universidad Central de Venezuela Facultad de Ciencias

    E-Print Network [OSTI]

    Coto, Ernesto

    las construcciones pueden ser traducidas fácilmente a la mayoría de los lenguajes de programación

  13. Universidad Central de Venezuela Facultad de Ciencias

    E-Print Network [OSTI]

    Cardinale Villarreal, Yudith C.

    #12;La Tecnología Verde, Green Computing o Green IT Algunos conceptos Huella energética (energy de la Computación Composición de Servicios Web La Tecnología Verde, Green Computing o Green tóxicos sin posibilidad de reciclaje) Green Computing o Computación Verde(informática ecológicamente

  14. Caracas, Venezuela: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar Energy LLC JumpBiossenceBrunswick,CalendarFork ElectricCaparaOpen

  15. Venezuela: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION JEnvironmental Jump to:EA EISTJThin FilmUnitedVairex Corporation JumpVaronManagement Inc Jump

  16. Protocadherin-7 induces bone metastasis of breast cancer

    SciTech Connect (OSTI)

    Li, Ai-Min; Tian, Ai-Xian; Zhang, Rui-Xue; Ge, Jie; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Sun, Xuan; Cao, Xu-Chen; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin

    2013-07-05

    Highlights: •PCDH7 is overexpression in high bone metastatic MDA-MB-231 cells. •PCDH7 is up-regulation in bone metastatic breast cancer tissues. •Suppression of PCDH7 inhibits cell proliferation, migration, and invasion in vitro. •PCDH7 induces breast cancer bone metastasis in vivo. -- Abstract: Breast cancer had a propensity to metastasize to bone, resulting in serious skeletal complications associated with poor outcome. Previous study showed that Protocadherin-7 (PCDH7) play an important role in brain metastatic breast cancer, however, the role of PCDH7 in bone metastatic breast cancer has never been explored. In the present study, we found that PCDH7 expression was up-regulation in bone metastatic breast cancer tissues by real-time PCR and immunohistochemistry assays. Furthermore, suppression of PCDH7 inhibits breast cancer cell proliferation, migration, and invasion in vitro by MTT, scratch, and transwell assays. Most importantly, overexpression of PCDH7 promotes breast cancer cell proliferation and invasion in vitro, and formation of bone metastasis in vivo. These data provide an important insight into the role of PCDH7 in bone metastasis of breast cancer.

  17. P70S6K 1 regulation of angiogenesis through VEGF and HIF-1{alpha} expression

    SciTech Connect (OSTI)

    Bian, Chuan-Xiu; Shi, Zhumei; Meng, Qiao; Jiang, Yue; Liu, Ling-Zhi; Jiang, Bing-Hua; Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107

    2010-07-30

    Research highlights: {yields} P70S6K1 regulates VEGF expression; {yields} P70S6K1 induces transcriptional activation through HIF-1{alpha} binding site; {yields} P70S6K1 regulates HIF-1{alpha}, but not HIF-1{beta} protein expression; {yields} P70S6K1 mediates tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression. -- Abstract: The 70 kDa ribosomal S6 kinase 1 (p70S6K1), a downstream target of phosphoinositide 3-kinase (PI3K) and ERK mitogen-activated protein kinase (MAPK), is an important regulator of cell cycle progression, and cell proliferation. Recent studies indicated an important role of p70S6K1 in PTEN-negative and AKT-overexpressing tumors. However, the mechanism of p70S6K1 in tumor angiogenesis remains to be elucidated. In this study, we specifically inhibited p70S6K1 activity in ovarian cancer cells using vector-based small interfering RNA (siRNA) against p70S6K1. We found that knockdown of p70S6K1 significantly decreased VEGF protein expression and VEGF transcriptional activation through the HIF-1{alpha} binding site at its enhancer region. The expression of p70S6K1 siRNA specifically inhibited HIF-1{alpha}, but not HIF-1{beta} protein expression. We also found that p70S6K1 down-regulation inhibited ovarian tumor growth and angiogenesis, and decreased cell proliferation and levels of VEGF and HIF-1{alpha} expression in tumor tissues. Our results suggest that p70S6K1 is required for tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression, providing a molecular mechanism of human ovarian cancer mediated by p70S6K1 signaling.

  18. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion

    E-Print Network [OSTI]

    Huang, Yu-Hwa

    T-cell immunoglobulin domain and mucin domain-3 (TIM-3, also known as HAVCR2) is an activation-induced inhibitory molecule involved in tolerance and shown to induce T-cell exhaustion in chronic viral infection and ...

  19. Hormonal regulation of FSH beta-subunit gene expression

    E-Print Network [OSTI]

    Hambarchyan, Arpi

    2011-01-01

    of development by directed oncogenesis in transgenic mice.induced by targeted oncogenesis using the SV40 T-antigen

  20. Dancing with the regulations - Part Deux

    SciTech Connect (OSTI)

    Nitschke, R.L.

    1995-12-31

    The disposal of low-level radioactive waste (LLW) in the United States has long been subjected to two very similar regulations depending upon the location. Disposal sites located on Department of Energy (DOE) Reservations are subject to DOE Order 5820.2A {open_quotes}Radioactive Waste Management,{close_quotes} while disposal sites located elsewhere are subject to the Nuclear Regulatory Commission regulation 10 CFR 61 {open_quotes}Licensing Requirements for Land Disposal of Radioactive Waste.{close_quotes} While life was not necessarily good, there was only one sheet of music to dance to. Recently a new player, named CERCLA (Comprehensive Environmental Response, Compensation, and Liability Act), has ridden into those DOE towns, and for those whose disposal facilities lie within or adjacent to Superfund sites, she has brought along a different drum to dance to. This paper discusses the differences and similarities between the different dance partners and their associated musical scores (i.e., the performance assessment (PA) required by the DOE order and the baseline risk assessment (BRA) required by CERCLA). The paper then provides a brief discussion on the latest dancer to cut in: the Defense Nuclear Facilities Safety Board (DNFSB). This discussion should help to alleviate the confusion while dancing on the LLW disposal regulatory ballroom floor.

  1. PI(5)P Regulates Autophagosome Biogenesis

    E-Print Network [OSTI]

    Vicinanza, Mariella; Korolchuk, Viktor I.; Ashkenazi, Avraham; Puri, Claudia; Menzies, Fiona M.; Clarke, Jonathan H.; Rubinsztein, David C.

    2015-01-08

    , 2015 ª2015 The Authors 227 (legend on next page) 228 Molecular Cell 57, 219–234, January 22, 2015 ª2015 The Authors and MTMR3 is the relevant lipid species for autophagosome generation during glucose starvation. Resveratrol induces auto- phagy... in noncanonical manner, independent of Beclin1 and partially resistant to Wm (Mauthe et al., 2011). The autophagy stimulation of resveratrol was impaired upon PI(5)P depletion due to overexpression of PI5P4K2a, b, and g (but not the catalytic-dead mutant of PI5P4K...

  2. Towards the Understanding of Induced Seismicity in Enhanced Geothermal...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Documents & Publications Towards the Understanding of Induced Seismicity in Enhanced Geothermal Systems Protocol for Addressing Induced Seismicity Associated with Enhanced...

  3. Interference of silibinin with IGF-1R signalling pathways protects human epidermoid carcinoma A431 cells from UVB-induced apoptosis

    SciTech Connect (OSTI)

    Liu, Weiwei; Otkur, Wuxiyar; Li, Lingzhi; Wang, Qiong; He, Hao; Zang, Linghe; Hayashi, Toshihiko [China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016 (China)] [China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016 (China); Tashiro, Shin-ichi [Institute for Clinical and Biomedical Sciences, Kyoto 603-8072 (Japan)] [Institute for Clinical and Biomedical Sciences, Kyoto 603-8072 (Japan); Onodera, Satoshi [Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo 194-8543 (Japan)] [Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo 194-8543 (Japan); Xia, Mingyu [China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016 (China)] [China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016 (China); Ikejima, Takashi, E-mail: ikejimat@vip.sina.com [China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016 (China)] [China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016 (China)

    2013-03-08

    Highlights: ? Silibinin protects A431 cells from UVB irradiation-induced apoptosis. ? Up-regulation of the IGF-1R-JNK/ERK pathways by UVB induces cell apoptosis. ? Silibinin inhibits IGF-1R pathways to repress caspase-8-mediated apoptosis. -- Abstract: Ultraviolet B (UVB) from sunlight is a major cause of cutaneous lesion. Silibinin, a traditional hepatic protectant, elicits protective effects against UVB-induced cellular damage. In A431 cells, the insulin-like growth factor-1 receptor (IGF-1R) was markedly up-regulated by UVB irradiation. The activation of the IGF-1R signalling pathways contributed to apoptosis of the cells rather than rescuing the cells from death. Up-regulated IGF-1R stimulated downstream mitogen-activated protein kinases (MAPKs), such as c-Jun N-terminal kinases (JNK) and extracellular signal-regulated protein kinases 1/2 (ERK1/2). The subsequent activation of caspase-8 and caspase-3 led to apoptosis. The activation of IGF-1R signalling pathways is the cause of A431 cell death. The pharmacological inhibitors and the small interfering RNA (siRNA) targeting IGF-1R suppressed the downstream activation of JNK/ERK-caspases to help the survival of the UVB-irradiated A431 cells. Indeed, silibinin treatment suppressed the IGF-1R-JNK/ERK pathways and thus protected the cells from UVB-induced apoptosis.

  4. Stimulation of CD107 affects LPS-induced cytokine secretion and cellular adhesion through the ERK signaling pathway in the human macrophage-like

    E-Print Network [OSTI]

    Lee, Won-Ha

    Stimulation of CD107 affects LPS-induced cytokine secretion and cellular adhesion through the ERK107 ERK Inflammation Signal transduction a b s t r a c t Lysosome-associated membrane proteins (LAMPs that extracellular signal-regulated kinase (ERK) mediates the regulatory action of CD107. These results suggest

  5. Effect of taurine on advanced glycation end products-induced hypertrophy in renal tubular epithelial cells

    SciTech Connect (OSTI)

    Huang, J.-S. [Department of Biological Science and Technology, Chung Hwa University of Medical Technology, Tainan 717, Taiwan (China)], E-mail: jaushyang12@hotmail.com; Chuang, L.-Y. [Department of Biochemistry, Kaohsiung Medical University, Kaohsiung, Taiwan (China); Guh, J.-Y. [Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (China); Yang, Y.-L.; Hsu, M.-S. [Department of Biological Science and Technology, Chung Hwa University of Medical Technology, Tainan 717, Taiwan (China)

    2008-12-01

    Mounting evidence indicates that advanced glycation end products (AGE) play a major role in the development of diabetic nephropathy (DN). Taurine is a well documented antioxidant agent. To explore whether taurine was linked to altered AGE-mediated renal tubulointerstitial fibrosis in DN, we examined the molecular mechanisms of taurine responsible for inhibition of AGE-induced hypertrophy in renal tubular epithelial cells. We found that AGE (but not non-glycated BSA) caused inhibition of cellular mitogenesis rather than cell death by either necrosis or apoptosis. There were no changes in caspase 3 activity, bcl-2 protein expression, and mitochondrial cytochrome c release in BSA, AGE, or the antioxidant taurine treatments in these cells. AGE-induced the Raf-1/extracellular signal-regulated kinase (ERK) activation was markedly blocked by taurine. Furthermore, taurine, the Raf-1 kinase inhibitor GW5074, and the ERK kinase inhibitor PD98059 may have the ability to induce cellular proliferation and cell cycle progression from AGE-treated cells. The ability of taurine, GW5074, or PD98059 to inhibit AGE-induced hypertrophy was verified by the observation that it significantly decreased cell size, cellular hypertrophy index, and protein levels of RAGE, p27{sup Kip1}, collagen IV, and fibronectin. The results obtained in this study suggest that taurine may serve as the potential anti-fibrotic activity in DN through mechanism dependent of its Raf-1/ERK inactivation in AGE-induced hypertrophy in renal tubular epithelial cells.

  6. The endocrine disruptor diethylstilbestrol induces adipocyte differentiation and promotes obesity in mice

    SciTech Connect (OSTI)

    Hao, Chan-Juan; Cheng, Xue-Jia; Xia, Hong-Fei, E-mail: hongfeixia@yahoo.com.cn; Ma, Xu

    2012-08-15

    Epidemiology studies indicate that exposure to endocrine disruptors during developmental “window” contributes to adipogenesis and the development of obesity. Implication of endocrine disruptor such as diethylstilbestrol (DES) on adipose tissue development has been poorly investigated. Here we evaluated the effects of DES on adipocyte differentiation in vitro and in vivo, and explored potential mechanism involved in its action. DES induced 3T3-L1 preadipocyte differentiation in a dose-dependent manner, and activated the expression of estrogen receptor (ER) and peroxisome proliferator-acivated receptor (PPAR) ? as well as its target genes required for adipogenesis in vitro. ER mediated the enhancement of DES-induced PPAR? activity. Moreover, DES perturbed key regulators of adipogenesis and lipogenic pathway in vivo. In utero exposure to low dose of DES significantly increased body weight, liver weight and fat mass in female offspring at postnatal day (PND) 60. In addition, serum triglyceride and glucose levels were also significantly elevated. These results suggest that perinatal exposure to DES may be expected to increase the incidence of obesity in a sex-dependent manner and can act as a potential chemical stressor for obesity and obesity-related disorders. -- Highlights: ? DES induced adipocyte differentiation in a dose-dependent manner in 3T3-L1 cells. ? DES activated adipogenic critical regulators and markers in vitro and in vivo. ? Perinatal exposure to DES led to the obese phenotype in female offspring. ? DES might be a potential chemical stressor for obesity and obesity-related disorders.

  7. A Nonperturbative Regulator for Chiral Gauge Theories

    E-Print Network [OSTI]

    Dorota M. Grabowska; David B. Kaplan

    2015-11-30

    We propose a nonperturbative gauge invariant regulator for $d$-dimensional chiral gauge theories on the lattice. The method involves simulating domain wall fermions in $d+1$ dimensions with quantum gauge fields that reside on one $d$-dimensional surface and are extended into the bulk via gradient flow. The result is a theory of gauged fermions plus mirror fermions, where the mirror fermions couple to the gauge fields via a form factor that becomes exponentially soft with the separation between domain walls. The resultant theory has a local $d$-dimensional interpretation only if the chiral fermion representation is anomaly free. A physical realization of this construction leads to mirror fermions in the Standard Model with soft form factors for gauge fields and possibly gravity. These mirror particles could evade detection and yet still be sensitive to vacuum topology, and could gravitate differently than conventional matter.

  8. Coherent regulation in yeast cell cycle network

    E-Print Network [OSTI]

    Nese Aral; Alkan Kabakcioglu

    2014-12-14

    We define a measure of coherent activity for gene regulatory networks, a property that reflects the unity of purpose between the regulatory agents with a common target. We propose that such harmonious regulatory action is desirable under a demand for energy efficiency and may be selected for under evolutionary pressures. We consider two recent models of the cell-cycle regulatory network of the budding yeast, Saccharomyces cerevisiae, as a case study and calculate their degree of coherence. A comparison with random networks of similar size and composition reveals that the yeast's cell-cycle regulation is wired to yield and exceptionally high level of coherent regulatory activity. We also investigate the mean degree of coherence as a function of the network size, connectivity and the fraction of repressory/activatory interactions.

  9. System and method for regulating resonant inverters

    DOE Patents [OSTI]

    Stevanovic, Ljubisa Dragoljub (Clifton Park, NY); Zane, Regan Andrew (Superior, CO)

    2007-08-28

    A technique is provided for direct digital phase control of resonant inverters based on sensing of one or more parameters of the resonant inverter. The resonant inverter control system includes a switching circuit for applying power signals to the resonant inverter and a sensor for sensing one or more parameters of the resonant inverter. The one or more parameters are representative of a phase angle. The resonant inverter control system also includes a comparator for comparing the one or more parameters to a reference value and a digital controller for determining timing of the one or more parameters and for regulating operation of the switching circuit based upon the timing of the one or more parameters.

  10. A Test Bed for Self-regulating Distribution Systems: Modeling Intergrated Renewable Energy and Demand Response in the GridLAB-D/MATLAB Environment

    SciTech Connect (OSTI)

    Wang, Dan; de Wit, Braydon; Parkinson, Simon; Fuller, Jason C.; Chassin, David P.; Crawford, Curran; Djilali, Ned

    2012-01-16

    This paper discusses the development of a simulation test bed permitting the study of integrated renewable energy generators and controlled distributed heat pumps operating within distribution systems. The test bed is demonstrated in this paper by addressing the important issue of the self-regulating effect of consumer-owned air-source heat pumps on the variability induced by wind power integration, particularly when coupled with increased access to demand response realized through a centralized load control strategy.

  11. Ion-induced nuclear radiotherapy

    DOE Patents [OSTI]

    Horn, Kevin M. (Albuquerque, NM); Doyle, Barney L. (Albuquerque, NM)

    1996-01-01

    Ion-induced Nuclear Radiotherapy (INRT) is a technique for conducting radiosurgery and radiotherapy with a very high degree of control over the spatial extent of the irradiated volume and the delivered dose. Based upon the concept that low energy, ion induced atomic and nuclear reactions can be used to produce highly energetic reaction products at the site of a tumor, the INRT technique is implemented through the use of a conduit-needle or tube which conducts a low energy ion beam to a position above or within the intended treatment area. At the end of the conduit-needle or tube is a specially fabricated target which, only when struck by the ion beam, acts as a source of energetic radiation products. The inherent limitations in the energy, and therefore range, of the resulting reaction products limits the spatial extent of irradiation to a pre-defined volume about the point of reaction. Furthermore, since no damage is done to tissue outside this irradiated volume, the delivered dose may be made arbitrarily large. INRT may be used both as a point-source of radiation at the site of a small tumor, or as a topical bath of radiation to broad areas of diseased tissue.

  12. Ion-induced nuclear radiotherapy

    DOE Patents [OSTI]

    Horn, K.M.; Doyle, B.L.

    1996-08-20

    Ion-induced Nuclear Radiotherapy (INRT) is a technique for conducting radiosurgery and radiotherapy with a very high degree of control over the spatial extent of the irradiated volume and the delivered dose. Based upon the concept that low energy, ion induced atomic and nuclear reactions can be used to produce highly energetic reaction products at the site of a tumor, the INRT technique is implemented through the use of a conduit-needle or tube which conducts a low energy ion beam to a position above or within the intended treatment area. At the end of the conduit-needle or tube is a specially fabricated target which, only when struck by the ion beam, acts as a source of energetic radiation products. The inherent limitations in the energy, and therefore range, of the resulting reaction products limits the spatial extent of irradiation to a pre-defined volume about the point of reaction. Furthermore, since no damage is done to tissue outside this irradiated volume, the delivered dose may be made arbitrarily large. INRT may be used both as a point-source of radiation at the site of a small tumor, or as a topical bath of radiation to broad areas of diseased tissue. 25 figs.

  13. Extracellular signal-regulated kinase 2 (ERK-2) mediated phosphorylation regulates nucleo-cytoplasmic shuttling and cell growth control of Ras-associated tumor suppressor protein, RASSF2

    SciTech Connect (OSTI)

    Kumari, Gita [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India)] [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India); Mahalingam, S., E-mail: mahalingam@iitm.ac.in [Laboratory of Molecular Virology, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500076 (India); Department of Biotechnology, Laboratory of Molecular Virology and Cell Biology, Indian Institute of Technology-Madras, Chennai 600 036 (India)

    2009-10-01

    Ras GTPase controls the normal cell growth through binding with an array of effector molecules, such as Raf and PI3-kinase in a GTP-dependent manner. RASSF2, a member of the Ras association domain family, is known to be involved in the suppression of cell growth and is frequently down-regulated in various tumor tissues by promoter hypermethylation. In the present study, we demonstrate that RASSF2 shuttles between nucleus and cytoplasm by a signal-mediated process and its export from the nucleus is sensitive to leptomycin B. Amino acids between 240 to 260 in the C-terminus of RASSF2 harbor a functional nuclear export signal (NES), which is necessary and sufficient for efficient export of RASSF2 from the nucleus. Substitution of conserved Ile254, Val257 and Leu259 within the minimal NES impaired RASSF2 export from the nucleus. In addition, wild type but not the nuclear export defective RASSF2 mutant interacts with export receptor, CRM-1 and exported from the nucleus. Surprisingly, we observed nucleolar localization for the nuclear export defective mutant suggesting the possibility that RASSF2 may localize in different cellular compartments transiently in a cell cycle dependent manner and the observed nuclear localization for wild type protein may be due to faster export kinetics from the nucleolus. Furthermore, our data suggest that RASSF2 is specifically phosphorylated by MAPK/ERK-2 and the inhibitors of MAPK pathway impair the phosphorylation and subsequently block the export of RASSF2 from the nucleus. These data clearly suggest that ERK-2 mediated phosphorylation plays an important role in regulating the nucleo-cytoplasmic shuttling of RASSF2. Interestingly, nuclear import defective mutant of RASSF2 failed to induce cell cycle arrest at G1/S phase and apoptosis suggesting that RASSF2 regulates cell growth in a nuclear localization dependent manner. Collectively, these data provided evidence for the first time that MAPK/ERK-2 mediated phosphorylation regulates nucleo-cytoplasmic transport and cell growth arrest activity of RASSF2. Taken together, the present study suggests that active transport between nucleus and cytoplasm may constitute an important regulatory mechanism for RASSF2 function.

  14. Air Pollution Control Regulations: No.27 - Control of Nitrogen...

    Broader source: Energy.gov (indexed) [DOE]

    Commercial Industrial Investor-Owned Utility MunicipalPublic Utility Rural Electric Cooperative Utility Program Info State Rhode Island Program Type Environmental Regulations...

  15. Air Pollution Control Regulations: No. 6 - Continuous Emissions...

    Broader source: Energy.gov (indexed) [DOE]

    Commercial Industrial Investor-Owned Utility MunicipalPublic Utility Rural Electric Cooperative Utility Program Info State Rhode Island Program Type Environmental Regulations...

  16. Air Pollution Control Regulations: No. 7 - Emission of Air Contaminant...

    Broader source: Energy.gov (indexed) [DOE]

    with the enjoyment of life and property. The criteria for determining compliance is listed in the regulations, and is based on other air pollution and ambient air standards...

  17. Economics of Lifecycle analysis and greenhouse gas regulations

    E-Print Network [OSTI]

    Rajagopal, Deepak

    2009-01-01

    4 Greenhouse gas regulation of fuels: intensity standardsfor biofuels increases greenhouse gases through emissionsI. de Carvalho Macedo. Greenhouse gas emissions and energy

  18. Regulated Emissions from Diesel and Compressed Natural Gas Transit...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Emissions from Diesel and Compressed Natural Gas Transit Buses Regulated Emissions from Diesel and Compressed Natural Gas Transit Buses Poster presentaiton at the 2007 Diesel...

  19. Rules and Regulations Pertaining to the Management of Wastes (Nebraska)

    Broader source: Energy.gov [DOE]

    These regulations, promulgated by the Department of Environmental Quality, contain provisions pertaining to waste management permits and licenses, wastewater, and the release of hazardous substances.

  20. Air Emission Regulations for the Prevention, Abatement, and Control...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Siting and Permitting Provider Department of Environmental Quality The Air Emission Regulation for the Prevention, Abatement and Control of Air Contaminants is relevant to all...