National Library of Energy BETA

Sample records for venezuela regulation induced

  1. LPG in Venezuela

    SciTech Connect (OSTI)

    Romero, O.

    1986-01-01

    The use of LPG for domestic consumption in Venezuela began in late 1929 when LPG was imported in lots of 500 cylinders. These cylinders were then returned to the U.S. for refilling. Total consumption at that time was some 40M/sup 3/ (250 barrels) per year and by 1937 had grown to some 540M/sup 3/ (3,400 barrels) per year. Local production of LPG from gas began in the mid thirties with a small cooling plant in the Mene Grande Field in the Lake Maracaibo area, the first field to produce oil in Venezuela (1914). This plant produced gasoline for a refinery and some of the first LPG used in Venezuela for domestic consumption. The capacity of this plant was insufficient to satisfy the growing demand for LPG which was supplied from refinery production until the development of the natural gas processing industry. At the present time, Venezuelan refineries are net consumers of LPG.

  2. Venezuela`s gas industry poised for long term growth

    SciTech Connect (OSTI)

    Croft, G.D.

    1995-06-19

    Venezuela`s enormous gas resource, combined with a new willingness to invite outside investment, could result in rapid growth in that industry into the next century. The development of liquefied natural gas exports will depend on the future course of gas prices in the US and Europe, but reserves are adequate to supply additional projects beyond the proposed Cristobal Colon project. Venezuela`s gas reserves are likely to increase if exploration for nonassociated gas is undertaken on a larger scale. The paper discusses gas reserves in Venezuela, internal gas markets, the potential for exports, competition from Trinidad, LNG export markets, and the encouragement of foreign investment in the gas industry of Venezuela.

  3. Caracas, Venezuela: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Caracas, Venezuela: Energy Resources Jump to: navigation, search Name Caracas, Venezuela Equivalent URI DBpedia GeoNames ID 3646738 Coordinates 10.5, -66.916667 Show Map...

  4. Estrogen induced {beta}-1,4-galactosyltransferase 1 expression regulates

    Office of Scientific and Technical Information (OSTI)

    proliferation of human breast cancer MCF-7 cells (Journal Article) | SciTech Connect Estrogen induced {beta}-1,4-galactosyltransferase 1 expression regulates proliferation of human breast cancer MCF-7 cells Citation Details In-Document Search Title: Estrogen induced {beta}-1,4-galactosyltransferase 1 expression regulates proliferation of human breast cancer MCF-7 cells Highlights: Black-Right-Pointing-Pointer We examined the regulation and biological functions of B4GALT1 expression induced

  5. Reformulated gasoline deal with Venezuela draws heat

    SciTech Connect (OSTI)

    Begley, R.

    1994-04-06

    A fight is brewing in Congress over a deal to let Venezuela off the hook in complying with the Clean Air Act reformulated gasoline rule. When Venezuela threatened to call for a GATT panel to challenge the rule as a trade barrier, the Clinton Administration negotiated to alter the rule, a deal that members of Congress are characterizing as {open_quotes}secret{close_quotes} and {open_quotes}back door.{close_quotes}

  6. MicroRNA Regulation of Ionizing Radiation-Induced Premature Senescence

    SciTech Connect (OSTI)

    Wang Yong; Scheiber, Melissa N.; Neumann, Carola; Calin, George A.; Zhou Daohong

    2011-11-01

    Purpose: MicroRNAs (miRNAs) have emerged as critical regulators of many cellular pathways. Ionizing radiation (IR) exposure causes DNA damage and induces premature senescence. However, the role of miRNAs in IR-induced senescence has not been well defined. Thus, the purpose of this study was to identify and characterize senescence-associated miRNAs (SA-miRNAs) and to investigate the role of SA-miRNAs in IR-induced senescence. Methods and Materials: In human lung (WI-38) fibroblasts, premature senescence was induced either by IR or busulfan (BU) treatment, and replicative senescence was accomplished by serial passaging. MiRNA microarray were used to identify SA-miRNAs, and real-time reverse transcription (RT)-PCR validated the expression profiles of SA-miRNAs in various senescent cells. The role of SA-miRNAs in IR-induced senescence was characterized by knockdown of miRNA expression, using anti-miRNA oligonucleotides or by miRNA overexpression through the transfection of pre-miRNA mimics. Results: We identified eight SA-miRNAs, four of which were up-regulated (miR-152, -410, -431, and -493) and four which were down-regulated (miR-155, -20a, -25, and -15a), that are differentially expressed in both prematurely senescent (induced by IR or BU) and replicatively senescent WI-38 cells. Validation of the expression of these SA-miRNAs indicated that down-regulation of miR-155, -20a, -25, and -15a is a characteristic miRNA expression signature of cellular senescence. Functional analyses revealed that knockdown of miR-155 or miR-20a, but not miR-25 or miR-15a, markedly enhanced IR-induced senescence, whereas ectopic overexpression of miR-155 or miR-20a significantly inhibited senescence induction. Furthermore, our studies indicate that miR-155 modulates IR-induced senescence by acting downstream of the p53 and p38 mitogen-activated protein kinase (MAPK) pathways and in part via regulating tumor protein 53-induced nuclear protein 1 (TP53INP1) expression. Conclusion: Our results suggest that SA-miRNAs are involved in the regulation of IR-induced senescence, so targeting these miRNAs may be a novel approach for modulating cellular response to radiation exposure.

  7. Pros and cons of power combined cycle in Venezuela

    SciTech Connect (OSTI)

    Alvarez, C.; Hernandez, S.

    1997-09-01

    In Venezuela combined cycle power has not been economically attractive to electric utility companies, mainly due to the very low price of natural gas. Savings in cost of natural gas due to a higher efficiency, characteristic of this type of cycle, does not compensate additional investments required to close the simple cycle (heat recovery steam generator (HRSG) and steam turbine island). Low gas prices have contributed to create a situation characterized by investors` reluctance to commit capital in gas pipe lines and associated equipment. The Government is taking measures to improve economics. Recently (January 1, 1997), the Ministry of Energy and Mines raised the price of natural gas, and established a formula to tie its price to the exchange rate variation (dollar/bolivar) in an intent to stimulate investments in this sector. This is considered a good beginning after a price freeze for about three years. Another measure that has been announced is the implementation of a corporate policy of outsourcing to build new gas facilities such as pipe lines and measuring and regulation stations. Under these new circumstances, it seems that combined cycle will play an important role in the power sector. In fact, some power generation projects are considering building new plants using this technology. An economical comparative study is presented between simple and combined cycles power plant. Screening curves are showed with a gas price forecast based on the government decree recently issued, as a function of plant capacity factor.

  8. Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization

    SciTech Connect (OSTI)

    Fairman, James Wesley; Wijerathna, Sanath Ranjan; Ahmad, Md Faiz; Xu, Hai; Nakano, Ryo; Jha, Shalini; Prendergast, Jay; Welin, R. Martin; Flodin, Susanne; Roos, Annette; Nordlund, Pr; Li, Zongli; Walz, Thomas; Dealwis, Chris Godfrey

    2011-07-25

    Ribonucleotide reductase (RR) is an {alpha}{sub n}{beta}{sub n} (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the {alpha}{sub 6}-{beta}{beta}'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

  9. Structural Basis for Allosteric Regulation of Human Ribonucleotide Reductase by Nucleotide-induced Oligomerization

    SciTech Connect (OSTI)

    J Fairman; S Wijerathna; M Ahmad; H Xu; R nakano; S jha; J Prendergast; R Welin; S Flodin; et al.

    2011-12-31

    Ribonucleotide reductase (RR) is an {alpha}{sub n}{beta}{sub n} (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the {alpha}{sub 6}-{beta}{beta}'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

  10. Residential energy use and conservation in Venezuela: Results and implications of a household survey in Caracas

    SciTech Connect (OSTI)

    Figueroa, M.J.; Ketoff, A.; Masera, O.

    1992-10-01

    This document presents the final report of a study of residential energy use in Caracas, the capital of Venezuela. It contains the findings of a household energy-use survey held in Caracas in 1988 and examines options for introducing energy conservation measures in the Venezuelan residential sector. Oil exports form the backbone of the Venezuelan economy. Improving energy efficiency in Venezuela will help free domestic oil resources that can be sold to the rest of the world. Energy conservation will also contribute to a faster recovery of the economy by reducing the need for major investments in new energy facilities, allowing the Venezuelan government to direct its financial investments towards other areas of development. Local environmental benefits will constitute an important additional by-product of implementing energy-efficiency policies in Venezuela. Caracas`s residential sector shows great potential for energy conservation. The sector is characterized by high saturation levels of major appliances, inefficiency of appliances available in the market, and by careless patterns of energy use. Household energy use per capita average 6.5 GJ/per year which is higher than most cities in developing countries; most of this energy is used for cooking. Electricity accounts for 41% of all energy use, while LPG and natural gas constitute the remainder. Specific options for inducing energy conservation and energy efficiency in Caracas`s residential sector include energy-pricing policies, fuel switching, particularly from electricity to gas, improving the energy performance of new appliances and customer information. To ensure the accomplishment of an energy-efficiency strategy, a concerted effort by energy users, manufacturers, utility companies, government agencies, and research institutions will be needed.

  11. Radioisotope Concentration in Lake Sediments of Maracaibo, Venezuela

    SciTech Connect (OSTI)

    Salas, A. Rangel; Viloria, T.; Sajo-Bohus, L.; Barros, H.; Greaves, E. D.; Palacios, D.

    2007-10-26

    Maracaibo Lake is one of the most important water basing and oil producing regions in Venezuela. Changes in the local environment have been monitored for chemical pollution in the past. For this study we selected a set of sediment samples collected in the shore and analyzed for its radioisotope content. Results show the gamma emitting isotopes distribution. Isotopes concentrations have been determined within the natural K, Th and U families.

  12. Venezuela No. 1 oil import source in S. America

    SciTech Connect (OSTI)

    Not Available

    1992-08-10

    This paper reports that with the exception of Venezuela, the U.S. is likely to import much oil from South American countries through 2010, the General Accounting Office reports. GAO, a congressional watchdog agency, noted the U.S. imports about 4% of its oil from Colombia, Ecuador, and Trinidad and Tobago and possibly could import from Argentina, Bolivia, Brazil, Chile, and Peru in the future. It the the eight countries' crude oil reserves are expected to increase about 30% by 2000, then slide about 2% by 2010. Their oil production is expected to climb about 21% over 1990 by 2000, then level off until 2010.

  13. Geology and geochemistry of crude oils, Bolivar coastal fields, Venezuela

    SciTech Connect (OSTI)

    Bockmeulen, H.; Barker, C.; Dickey, P.A.

    1983-02-01

    The Bolivar Coastal Fields (BCF) are located on the eastern margin of Lake Maracaibo, Venezuela. They form the largest oil field outside of the Middle East and contain mostly heavy oil with a gravity less than 22/sup 0/ API. Thirty crude oils from the BCF were collected along two parallel and generally southwest-northeast trends. These oils were characterized by their API gravity, percent saturates, aromatics, NSO and asphalitic compounds, gas chromatograms for whole oils, C/sub 4/-C/sub 7/ fractions, and aromatics. Also, 24 associated waters were sampled and analyzed for Ca/sup + +/, Mg/sup + +/, Na/sup +/, HCO/sub 3//sup -/, CO/sub 3//sup - -/, SO/sub 4//sup - -/, pH, and total dissolved solids (TDS). The geological and geochemical significances of these analyses are discussed with particular emphasis on the genesis of the petroleum.

  14. Five Years of Cyclotron Radioisotope Production Experiences at the First PET-CT in Venezuela

    SciTech Connect (OSTI)

    Colmenter, L.; Coelho, D.; Esteves, L. M.; Ruiz, N.; Morales, L.; Lugo, I.; Sajo-Bohus, L.; Liendo, J. A.; Greaves, E. D.; Barros, H.; Castillo, J.

    2007-10-26

    Five years operation of a compact cyclotron installed at PET-CT facility in Caracas, Venezuela is given. Production rate of {sup 18}F labeled FDG, operation and radiation monitoring experience are included. We conclude that {sup 18}FDG CT-PET is the most effective technique for patient diagnosis.

  15. Household energy use in urban Venezuela: Implications from surveys in Maracaibo, Valencia, Merida, and Barcelona-Puerto La Cruz

    SciTech Connect (OSTI)

    Figueroa, M.J.; Sathaye, J.

    1993-08-01

    This report identifies the most important results of a comparative analysis of household commercial energy use in Venezuelan urban cities. The use of modern fuels is widespread among all cities. Cooking consumes the largest share of urban household energy use. The survey documents no use of biomass and a negligible use of kerosene for cooking. LPG, natural gas, and kerosene are the main fuels available. LPG is the fuel choice of low-income households in all cities except Maracaibo, where 40% of all households use natural gas. Electricity consumption in Venezuela`s urban households is remarkably high compared with the levels used in households in comparable Latin American countries and in households of industrialized nations which confront harsher climatic conditions and, therefore, use electricity for water and space heating. The penetration of appliances in Venezuela`s urban households is very high. The appliances available on the market are inefficient, and there are inefficient patterns of energy use among the population. Climate conditions and the urban built form all play important roles in determining the high level of energy consumption in Venezuelan urban households. It is important to acknowledge the opportunities for introducing energy efficiency and conservation in Venezuela`s residential sector, particularly given current economic and financial constraints, which may hamper the future provision of energy services.

  16. JBP485 improves gentamicin-induced acute renal failure by regulating the expression and function of Oat1 and Oat3 in rats

    SciTech Connect (OSTI)

    Guo, Xinjin [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Meng, Qiang; Liu, Qi; Wang, Changyuan; Sun, Huijun; Peng, Jinyong; Ma, Xiaochi [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Dalian Medical University, Liaoning (China); Kaku, Taiichi [Japan Bioproducts Industry Co. Ltd., Tomigaya, Shibuya-ku, Tokyo (Japan); Liu, Kexin, E-mail: kexinliu@dlmedu.edu.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Dalian Medical University, Liaoning (China)

    2013-09-01

    We investigated the effects of JBP485 (an anti-inflammatory dipeptide and a substrate of OAT) on regulation of the expression and function of renal Oat1 and Oat3, which can accelerate the excretion of accumulated uremic toxins (e.g. indoxyl sulfate) in the kidney to improve gentamicin-induced ARF in rats. JBP485 caused a significant decrease in the accumulation of endogenous substances (creatinine, blood urea nitrogen and indoxyl sulfate) in vivo, an increase in the excretion of exogenous compounds (lisinopril and inulin) into urine, and up-regulation of the expressions of renal Oat1 and Oat3 in the kidney tissues and slices via substrate induction. To determine the effect of JBP485 on the accelerated excretion of uremic toxins mediated by Oat1 and Oat3, the mRNA and protein expression levels of renal basolateral Oats were assessed by quantitative real-time PCR, western blot, immunohistochemical analysis and an immunofluorescence method. Gentamicin down-regulated the expression of Oats mRNA and protein in rat kidney, and these effects were reversed after administration of JBP485. In addition, JBP485 caused a significant decrease in MPO and MDA levels in the kidney, and improved the pathological condition of rat kidney. These results indicated that JBP485 improved acute renal failure by increasing the expression and function of Oat1 and Oat3, and by decreasing overoxidation of the kidney in gentamicin-induced ARF rats. - Highlights: JBP485 could up-regulate function and expression of Oat1 and Oat3 in kidney. Effects of JBP485 on ARF are mediated by stimulating excretion of uremic toxins. JBP485 protected against gentamicin-induced ARF by decreasing MPO and MDA.

  17. Moderate extracellular acidification inhibits capsaicin-induced cell death through regulating calcium mobilization, NF-{kappa}B translocation and ROS production in synoviocytes

    SciTech Connect (OSTI)

    Hu, Fen; Yang, Shuang; Zhao, Dan; Zhu, Shuyan; Wang, Yuxiang; Li, Junying

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Moderate extracellular acidification regulates intracellular Ca{sup 2+} mobilization. Black-Right-Pointing-Pointer Moderate acidification activates NF-{kappa}B nuclear translocation in synoviocytes. Black-Right-Pointing-Pointer Moderate acidification depresses the ROS production induced by capsaicin. Black-Right-Pointing-Pointer Moderate acidification inhibits capsaicin-caused synoviocyte death. -- Abstract: We previously show the expression of transient receptor potential vanilloid 1 (TRPV1) in primary synoviocytes from collagen-induced arthritis (CIA) rats. Capsaicin and lowered extracellular pH from 7.4 to 5.5 induce cell death through TRPV1-mediated Ca{sup 2+} entry and reactive oxygen species (ROS) production. However, under the pathological condition in rheumatoid arthritis, the synovial fluid is acidified to a moderate level (about pH 6.8). In the present study, we examined the effects of pH 6.8 on the TRPV1-mediated cell death. Our finding is different or even opposite from what was observed at pH 5.5. We found that the moderate extracellular acidification (from pH 7.4 to 6.8) inhibited the capsaicin-induced Ca{sup 2+} entry through attenuating the activity of TRPV1. In the mean time, it triggered a phospholipse C (PLC)-related Ca{sup 2+} release from intracellular stores. The nuclear translocation of NF-{kappa}B was found at pH 6.8, and this also depends on PLC activation. Moreover, the capsaicin-evoked massive ROS production and cell death were depressed at pH 6.8, both of which are dependent on the activation of PLC and NF-{kappa}B. Taken together, these results suggested that the moderate extracellular acidification inhibited the capsaicin-induced synoviocyte death through regulating Ca{sup 2+} mobilization, activating NF-{kappa}B nuclear translocation and depressing ROS production.

  18. Copper deficiency alters cell bioenergetics and induces mitochondrial fusion through up-regulation of MFN2 and OPA1 in erythropoietic cells

    SciTech Connect (OSTI)

    Bustos, Rodrigo I.; Jensen, Erik L.; Ruiz, Lina M.; Rivera, Salvador; Ruiz, Sebastin; Simon, Felipe; Riedel, Claudia; Millennium Institute of Immunology and Immunotherapy, Santiago ; Ferrick, David; Elorza, Alvaro A.

    2013-08-02

    Highlights: In copper deficiency, cell proliferation is not affected. In turn, cell differentiation is impaired. Enlarged mitochondria are due to up-regulation of MNF2 and OPA1. Mitochondria turn off respiratory chain and ROS production. Energy metabolism switch from mitochondria to glycolysis. -- Abstract: Copper is essential in cell physiology, participating in numerous enzyme reactions. In mitochondria, copper is a cofactor for respiratory complex IV, the cytochrome c oxidase. Low copper content is associated with anemia and the appearance of enlarged mitochondria in erythropoietic cells. These findings suggest a connection between copper metabolism and bioenergetics, mitochondrial dynamics and erythropoiesis, which has not been explored so far. Here, we describe that bathocuproine disulfonate-induced copper deficiency does not alter erythropoietic cell proliferation nor induce apoptosis. However it does impair erythroid differentiation, which is associated with a metabolic switch between the two main energy-generating pathways. That is, from mitochondrial function to glycolysis. Switching off mitochondria implies a reduction in oxygen consumption and ROS generation along with an increase in mitochondrial membrane potential. Mitochondrial fusion proteins MFN2 and OPA1 were up-regulated along with the ability of mitochondria to fuse. Morphometric analysis of mitochondria did not show changes in total mitochondrial biomass but rather bigger mitochondria because of increased fusion. Similar results were also obtained with human CD34+, which were induced to differentiate into red blood cells. In all, we have shown that adequate copper levels are important for maintaining proper mitochondrial function and for erythroid differentiation where the energy metabolic switch plus the up-regulation of fusion proteins define an adaptive response to copper deprivation to keep cells alive.

  19. Aspirin-triggered resolvin D1 down-regulates inflammatory responses and protects against endotoxin-induced acute kidney injury

    SciTech Connect (OSTI)

    Chen, Jiao; Shetty, Sreerama; Zhang, Ping; Gao, Rong; Hu, Yuxin; Wang, Shuxia; Li, Zhenyu; Fu, Jian

    2014-06-01

    The presence of endotoxin in blood can lead to acute kidney injury (AKI) and septic shock. Resolvins, the endogenous lipid mediators derived from docosahexaenoic acid, have been reported to exhibit potent anti-inflammatory action. Using a mouse model of lipopolysaccharide (LPS)-induced AKI, we investigated the effects of aspirin-triggered resolvin D1 (AT-RvD1) on inflammatory kidney injury. Administration of AT-RvD1 1 h after LPS challenge protected the mice from kidney injury as indicated by the measurements of blood urea nitrogen, serum creatinine, and morphological alterations associated with tubular damage. The protective effects were evidenced by decreased neutrophil infiltration in the kidney indicating reduction in inflammation. AT-RvD1 treatment restored kidney cell junction protein claudin-4 expression, which was otherwise reduced after LPS challenge. AT-RvD1 treatment inhibited endotoxin-induced NF-κB activation and suppressed LPS-induced ICAM-1 and VCAM-1 expression in the kidney. Moreover, AT-RvD1 treatment markedly decreased LPS-induced IL-6 level in the kidney and blocked IL-6-mediated signaling including STAT3 and ERK phosphorylation. Our findings demonstrate that AT-RvD1 is a potent anti-inflammatory mediator in LPS-induced kidney injury, and AT-RvD1 has therapeutic potential against AKI during endotoxemia.

  20. Lipid rafts regulate PCB153-induced disruption of occludin and brain endothelial barrier function through protein phosphatase 2A and matrix metalloproteinase-2

    SciTech Connect (OSTI)

    Eum, Sung Yong Jaraki, Dima; András, Ibolya E.; Toborek, Michal

    2015-09-15

    Occludin is an essential integral transmembrane protein regulating tight junction (TJ) integrity in brain endothelial cells. Phosphorylation of occludin is associated with its localization to TJ sites and incorporation into intact TJ assembly. The present study is focused on the role of lipid rafts in polychlorinated biphenyl (PCB)-induced disruption of occludin and endothelial barrier function. Exposure of human brain endothelial cells to 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB153) induced dephosphorylation of threonine residues of occludin and displacement of occludin from detergent-resistant membrane (DRM)/lipid raft fractions within 1 h. Moreover, lipid rafts modulated the reduction of occludin level through activation of matrix metalloproteinase 2 (MMP-2) after 24 h PCB153 treatment. Inhibition of protein phosphatase 2A (PP2A) activity by okadaic acid or fostriecin markedly protected against PCB153-induced displacement of occludin and increased permeability of endothelial cells. The implication of lipid rafts and PP2A signaling in these processes was further defined by co-immunoprecipitation of occludin with PP2A and caveolin-1, a marker protein of lipid rafts. Indeed, a significant MMP-2 activity was observed in lipid rafts and was increased by exposure to PCB153. The pretreatment of MMP-2 inhibitors protected against PCB153-induced loss of occludin and disruption of lipid raft structure prevented the increase of endothelial permeability. Overall, these results indicate that lipid raft-associated processes, such as PP2A and MMP-2 activation, participate in PCB153-induced disruption of occludin function in brain endothelial barrier. This study contributes to a better understanding of the mechanisms leading to brain endothelial barrier dysfunction in response to exposure to environmental pollutants, such as ortho-substituted PCBs. - Highlights: • PCB153 disturbed human brain endothelial barrier through disruption of occludin. • Lipid raft-associated PP2A/MMP-2 induced PCB153-induced dysfunction of occludin. • Disrupted lipid rafts modulated PCB153-induced increase of permeability. • Lipid rafts act as a signaling platform for PCB153-induced dysfunction of occludin.

  1. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  2. EGCG protects endothelial cells against PCB 126-induced inflammation through inhibition of AhR and induction of Nrf2-regulated genes

    SciTech Connect (OSTI)

    Han, Sung Gu; Department of Animal and Food Sciences, College of Agriculture, University of Kentucky, Lexington, KY 40536 ; Han, Seong-Su; Toborek, Michal; Hennig, Bernhard; Department of Animal and Food Sciences, College of Agriculture, University of Kentucky, Lexington, KY 40536

    2012-06-01

    Tea flavonoids such as epigallocatechin gallate (EGCG) protect against vascular diseases such as atherosclerosis via their antioxidant and anti-inflammatory functions. Persistent and widespread environmental pollutants, including polychlorinated biphenyls (PCB), can induce oxidative stress and inflammation in vascular endothelial cells. Even though PCBs are no longer produced, they are still detected in human blood and tissues and thus considered a risk for vascular dysfunction. We hypothesized that EGCG can protect endothelial cells against PCB-induced cell damage via its antioxidant and anti-inflammatory properties. To test this hypothesis, primary vascular endothelial cells were pretreated with EGCG, followed by exposure to the coplanar PCB 126. Exposure to PCB 126 significantly increased cytochrome P450 1A1 (Cyp1A1) mRNA and protein expression and superoxide production, events which were significantly attenuated following pretreatment with EGCG. Similarly, EGCG also reduced DNA binding of NF-κB and downstream expression of inflammatory markers such as monocyte chemotactic protein-1 (MCP-1) and vascular cell adhesion protein-1 (VCAM-1) after PCB exposure. Furthermore, EGCG decreased endogenous or base-line levels of Cyp1A1, MCP-1 and VCAM-1 in endothelial cells. Most of all, treatment of EGCG upregulated expression of NF-E2-related factor 2 (Nrf2)-controlled antioxidant genes, including glutathione S transferase (GST) and NAD(P)H:quinone oxidoreductase 1 (NQO1), in a dose-dependent manner. In contrast, silencing of Nrf2 increased Cyp1A1, MCP-1 and VCAM-1 and decreased GST and NQO1 expression, respectively. These data suggest that EGCG can inhibit AhR regulated genes and induce Nrf2-regulated antioxidant enzymes, thus providing protection against PCB-induced inflammatory responses in endothelial cells. -- Highlights: ► PCBs cause endothelial inflammation and subsequent atherosclerosis. ► Nutrition can modulate toxicity by environmental pollutants. ► We demonstrated that EGCG can decrease PCB-induced inflammation. ► EGCG protection was via inhibition of AhR and induction of Nrf2 regulatory genes.

  3. Radiation-Induced Upregulation of Gene Expression From Adenoviral Vectors Mediated by DNA Damage Repair and Regulation

    SciTech Connect (OSTI)

    Nokisalmi, Petri; Rajecki, Maria; Pesonen, Sari; Escutenaire, Sophie; Soliymani, Rabah; Tenhunen, Mikko; Ahtiainen, Laura; Hemminki, Akseli

    2012-05-01

    Purpose: In the present study, we evaluated the combination of replication-deficient adenoviruses and radiotherapy in vitro. The purpose of the present study was to analyze the mechanism of radiation-mediated upregulation of adenoviral transgene expression. Methods and Materials: Adenoviral transgene expression (luciferase or green fluorescent protein) was studied with and without radiation in three cell lines: breast cancer M4A4-LM3, prostate cancer PC-3MM2, and lung cancer LNM35/enhanced green fluorescent protein. The effect of the radiation dose, modification of the viral capsid, and five different transgene promoters were studied. The cellular responses were studied using mass spectrometry and immunofluorescence analysis. Double strand break repair was modulated by inhibitors of heat shock protein 90, topoisomerase-I, and DNA protein kinase, and transgene expression was measured. Results: We found that a wide range of radiation doses increased adenoviral transgene expression regardless of the cell line, transgene, promoter, or viral capsid modification. Treatment with adenovirus, radiation, and double strand break repair inhibitors resulted in persistence of double strand breaks and subsequent increases in adenovirus transgene expression. Conclusions: Radiation-induced enhancement of adenoviral transgene expression is linked to DNA damage recognition and repair. Radiation induces a global cellular response that results in increased production of RNA and proteins, including adenoviral transgene products. This study provides a mechanistic rationale for combining radiation with adenoviral gene delivery.

  4. Activation of PPAR{delta} up-regulates fatty acid oxidation and energy uncoupling genes of mitochondria and reduces palmitate-induced apoptosis in pancreatic {beta}-cells

    SciTech Connect (OSTI)

    Wan, Jun; Jiang, Li; Lue, Qingguo; Ke, Linqiu; Li, Xiaoyu; Tong, Nanwei

    2010-01-15

    Recent evidence indicates that decreased oxidative capacity, lipotoxicity, and mitochondrial aberrations contribute to the development of insulin resistance and type 2 diabetes. The goal of this study was to investigate the effects of peroxisome proliferator-activated receptor {delta} (PPAR{delta}) activation on lipid oxidation, mitochondrial function, and insulin secretion in pancreatic {beta}-cells. After HIT-T15 cells (a {beta}-cell line) were exposed to high concentrations of palmitate and GW501516 (GW; a selective agonist of PPAR{delta}), we found that administration of GW increased the expression of PPAR{delta} mRNA. GW-induced activation of PPAR{delta} up-regulated carnitine palmitoyltransferase 1 (CPT1), long-chain acyl-CoA dehydrogenase (LCAD), pyruvate dehydrogenase kinase 4 (PDK4), and uncoupling protein 2 (UCP2); alleviated mitochondrial swelling; attenuated apoptosis; and reduced basal insulin secretion induced by increased palmitate in HIT cells. These results suggest that activation of PPAR{delta} plays an important role in protecting pancreatic {beta}-cells against aberrations caused by lipotoxicity in metabolic syndrome and diabetes.

  5. Venezuela offshore oil and gas production development: Past, present and future

    SciTech Connect (OSTI)

    Perez La Salvia, H.; Schwartz, E.; Contreras, M.; Rodriguez, J.I.; Febres, G.; Gajardo, E.

    1995-12-01

    This paper presents a short history of offshore oil and gas production in Venezuela starting in Lake Maracaibo in 1923. The main emphasis has been the results of the recent R and D and the exploratory offshore programs in areas like Orinoco Delta located in the Atlantic Ocean, Northeast and Northwest Venezuela in the Caribbean sea. In the R and D offshore program the main objectives were: (1) To establish the local environmental, oceanographical, geotechnical and seismicity conditions for the Venezuelan Continental Platform. (2) To give a technical support to the PDVSA Operating Affiliates during the exploratory programs including: (a) to develop accurate drilling vessel positioning systems; (b) evaluation of sea bottom geotechnical conditions for safely operating the jack-ups and drilling vessels involved in the exploratory wells and (c) to identify those areas which because of their special nature require further investigation to establish preliminary type of platforms required for the areas to be developed or to evaluate other solutions proposed by Foreign Consultant Engineering Companies to the PDVSA Operating Affiliated Companies. The main objective of PDVSA for the coming future will be to develop the North of Paria Gas Field through the initially named Christopher Columbus Project now Sucre Gas, S.A., a consortium conformed by LaGoven, S.A. Shell, Exxon and Mitsubishi. objective of this paper is to give an idea of the history of the Venezuelan Oil and Gas Offshore development giving emphasis to the results of the INTEVEP S.A. Red offshore program and to show some results of the particular characteristics of oceanographical, environmental, geotechnical and seismic conditions in the main areas evaluated during the exploratory program: Orinoco Delta, Gulf of Paria and North of Paria.

  6. Cyanidin-3-glucoside inhibits UVB-induced oxidative damage and inflammation by regulating MAP kinase and NF-κB signaling pathways in SKH-1 hairless mice skin

    SciTech Connect (OSTI)

    Pratheeshkumar, Poyil; Son, Young-Ok; Wang, Xin; Divya, Sasidharan Padmaja; Joseph, Binoy; Hitron, John Andrew; Wang, Lei; Kim, Donghern; Yin, Yuanqin; Roy, Ram Vinod; Lu, Jian; Zhang, Zhuo; Wang, Yitao; and others

    2014-10-01

    Skin cancer is one of the most commonly diagnosed cancers in the United States. Exposure to ultraviolet-B (UVB) radiation induces inflammation and photocarcinogenesis in mammalian skin. Cyanidin-3-glucoside (C3G), a member of the anthocyanin family, is present in various vegetables and fruits especially in edible berries, and displays potent antioxidant and anticarcinogenic properties. In this study, we have assessed the in vivo effects of C3G on UVB irradiation induced chronic inflammatory responses in SKH-1 hairless mice, a well-established model for UVB-induced skin carcinogenesis. Here, we show that C3G inhibited UVB-induced skin damage and inflammation in SKH-1 hairless mice. Our results indicate that C3G inhibited glutathione depletion, lipid peroxidation and myeloperoxidation in mouse skin by chronic UVB exposure. C3G significantly decreased the production of UVB-induced pro-inflammatory cytokines, such as IL-6 and TNF-α, associated with cutaneous inflammation. Likewise, UVB-induced inflammatory responses were diminished by C3G as observed by a remarkable reduction in the levels of phosphorylated MAP kinases, Erk1/2, p38, JNK1/2 and MKK4. Furthermore, C3G also decreased UVB-induced cyclooxygenase-2 (COX-2), PGE{sub 2} and iNOS levels, which are well-known key mediators of inflammation and cancer. Treatment with C3G inhibited UVB-induced nuclear translocation of NF-κB and degradation of IκBα in mice skin. Immunofluorescence assay revealed that topical application of C3G inhibited the expression of 8-hydroxy-2′-deoxyguanosine, proliferating cell nuclear antigen, and cyclin D1 in chronic UVB exposed mouse skin. Collectively, these data indicates that C3G can provide substantial protection against the adverse effects of UVB radiation by modulating UVB-induced MAP kinase and NF-κB signaling pathways. - Highlights: • C3G inhibited UVB-induced oxidative damage and inflammation. • C3G inhibited UVB-induced COX-2, iNOS and PGE{sub 2} production. • C3G inhibited UVB-induced elevated proinflammatory cytokine level. • C3G inhibited UVB-induced p38 MAP kinase signaling. • C3G inhibited UVB-induced NF-κB activation.

  7. Venezuela-MEM/USA-DOE Fossil Energy Report IV-11: Supporting technology for enhanced oil recovery - EOR thermal processes

    SciTech Connect (OSTI)

    Venezuela

    2000-04-06

    This report contains the results of efforts under the six tasks of the Tenth Amendment anti Extension of Annex IV, Enhanced Oil Recovery Thermal Processes of the Venezuela/USA Energy Agreement. This report is presented in sections (for each of the six Tasks) and each section contains one or more reports that were prepared to describe the results of the effort under each of the Tasks. A statement of each Task, taken from the Agreement Between Project Managers, is presented on the first page of each section. The Tasks are numbered 68 through 73. The first through tenth report on research performed under Annex IV Venezuela MEM/USA-DOE Fossil Energy Report Number IV-1, IV-2, IV-3, IV-4, IV-5, IV-6, IV-7, IV-8, IV-9, IV-10 contain the results of the first 67 Tasks. These reports are dated April 1983, August 1984, March 1986, July 1987, November 1988, December 1989, October 1991, February 1993, March 1995, and December 1997, respectively.

  8. Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Information Center Regulations Regulations Personnel Security Regulations Click Here FOIA Regulations Click Here Privacy Act Regulations Click Here Whistleblower Protection ...

  9. VOLTAGE REGULATOR

    DOE Patents [OSTI]

    Von Eschen, R.L.; Scheele, P.F.

    1962-04-24

    A transistorized voltage regulator which provides very close voitage regulation up to about 180 deg F is described. A diode in the positive line provides a constant voltage drop from the input to a regulating transistor emitter. An amplifier is coupled to the positive line through a resistor and is connected between a difference circuit and the regulating transistor base which is negative due to the difference in voltage drop across thc diode and the resistor so that a change in the regulator output causes the amplifier to increase or decrease the base voltage and current and incrcase or decrease the transistor impedance to return the regulator output to normal. (AEC)

  10. {gamma}-aminobutyric acid{sub A} (GABA{sub A}) receptor regulates ERK1/2 phosphorylation in rat hippocampus in high doses of Methyl Tert-Butyl Ether (MTBE)-induced impairment of spatial memory

    SciTech Connect (OSTI)

    Zheng Gang; Zhang Wenbin; Zhang Yun; Chen Yaoming; Liu Mingchao; Yao Ting; Yang Yanxia; Zhao Fang; Li Jingxia; Huang Chuanshu; Luo Wenjing Chen Jingyuan

    2009-04-15

    Experimental and occupational exposure to Methyl Tert-Butyl Ether (MTBE) has been reported to induce neurotoxicological and neurobehavioral effects, such as headache, nausea, dizziness, and disorientation, etc. However, the molecular mechanisms involved in MTBE-induced neurotoxicity are still not well understood. In the present study, we investigated the effects of MTBE on spatial memory and the expression and function of GABA{sub A} receptor in the hippocampus. Our results demonstrated that intraventricular injection of MTBE impaired the performance of the rats in a Morris water maze task, and significantly increased the expression of GABA{sub A} receptor {alpha}1 subunit in the hippocampus. The phosphorylation of ERK1/2 decreased after the MTBE injection. Furthermore, the decreased ability of learning and the reduction of phosphorylated ERK1/2 level of the MTBE-treated rats was partly reversed by bicuculline injected 30 min before the training. These results suggested that MTBE exposure could result in impaired spatial memory. GABA{sub A} receptor may play an important role in the MTBE-induced impairment of learning and memory by regulating the phosphorylation of ERK in the hippocampus.

  11. Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function During Nanoparticle-Induced Oxidative Stress

    SciTech Connect (OSTI)

    Duan, Jicheng; Kodali, Vamsi K.; Gaffrey, Matthew J.; Guo, Jia; Chu, Rosalie K.; Camp, David G.; Smith, Richard D.; Thrall, Brian D.; Qian, Weijun

    2015-12-23

    Engineered nanoparticles (ENPs) are emerging functional materials increasingly utilized for commercial and medical applications. Due to the potential hazard effects of ENPs to human health, it is significant to assess and understand the underlying mechanisms of nanotoxicity. Herein, we investigate protein S-glutathionylation (SSG) as an underlying regulatory mechanism for ENP-induced oxidative stress in macrophages by applying a recently developed quantitative redox proteomics approach for site-specific measurements of SSG. Three high-volume production ENPs (SiO2, Fe3O4 and CoO) were selected as representative ENPs with low, moderate, and high reactive oxygen species (ROS) activity, respectively. Among these nanoparticles, we observe that CoO led to the most significant dose-dependent oxidative stress and increase of protein SSG modifications in macrophages. Our site-specific SSG changes highlighted a broad set of redox sensitive proteins and their specific Cys residues potentially implicated in stress response. Functional analysis revealed that the most significantly enriched functional categories for SSG-modified proteins were stress response, cellular structure change, and cell death or survival. Moreover, ENPs-induce oxidative stress levels (CoO > Fe3O4 > SiO2) were found to correlate well with the levels of impairment of macrophage phagocytic activity and the overall degrees of increases in SSG. RNA silencing knockdown experiment of glutaredoxin 1 (Grx1) also led to a decreased phagocytic activity in macrophages, which suggested a regulatory role of SSG in phagocytosis. Together, the results provided valuable insights of protein SSG as a potential regulatory mechanism in response to nanomaterial-induced oxidative stress and immunity dysfunction.

  12. Reciprocal Regulation of Hypoxia-Inducible Factor 2α and GLI1 Expression Associated With the Radioresistance of Renal Cell Carcinoma

    SciTech Connect (OSTI)

    Zhou, Jiancheng; Wu, Kaijie; Gao, Dexuan; Zhu, Guodong; Wu, Dapeng; Wang, Xinyang; Chen, Yule; Du, Yuefeng; Song, Wenbin; Ma, Zhenkun; Authement, Craig; Saha, Debabrata; Hsieh, Jer-Tsong; He, Dalin

    2014-11-15

    Purpose: Renal cell carcinoma (RCC) is often considered a radioresistant tumor, but the molecular mechanism underlying its radioresistance is poorly understood. This study explored the roles of hypoxia-inducible factor 2α (HIF2α) and sonic hedgehog (SHH)-GLI1 signaling in mediating the radioresistance of RCC cells and to unveil the interaction between these 2 signaling pathways. Methods and Materials: The activities of SHH-GLI1 signaling pathway under normoxia and hypoxia in RCC cells were examined by real-time polymerase chain reaction, Western blot, and luciferase reporter assay. The expression of HIF2α and GLI1 in RCC patients was examined by immunohistochemistry, and their correlation was analyzed. Furthermore, RCC cells were treated with HIF2α-specific shRNA (sh-HIF2α), GLI1 inhibitor GANT61, or a combination to determine the effect of ionizing radiation (IR) on RCC cells based on clonogenic assay and double-strand break repair assay. Results: RCC cells exhibited elevated SHH-GLI1 activities under hypoxia, which was mediated by HIF2α. Hypoxia induced GLI1 activation through SMO-independent pathways that could be ablated by PI3K inhibitor or MEK inhibitor. Remarkably, the SHH-GLI1 pathway also upregulated HIF2α expression in normoxia. Apparently, there was a positive correlation between HIF2α and GLI1 expression in RCC patients. The combination of sh-HIF2α and GLI1 inhibitor significantly sensitized RCC cells to IR. Conclusions: Cross-talk between the HIF2α and SHH-GLI1 pathways was demonstrated in RCC. Cotargeting these 2 pathways, significantly sensitizing RCC cells to IR, provides a novel strategy for RCC treatment.

  13. Acquisition Regulation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... for DOE until such time as the DOE Acquisition Regulation (DEAR) is amended to ... 2015. The clause provides the necessary language to require M&O contractors (to include ...

  14. Approaches to identifying reservoir heterogeneity and reserve growth opportunities from subsurface data: The Oficina Formation, Budare field, Venezuela

    SciTech Connect (OSTI)

    Hamilton, D.S.; Raeuchle, S.K.; Holtz, M.H.

    1997-08-01

    We applied an integrated geologic, geophysical, and engineering approach devised to identify heterogeneities in the subsurface that might lead to reserve growth opportunities in our analysis of the Oficina Formation at Budare field, Venezuela. The approach involves 4 key steps: (1) Determine geologic reservoir architecture; (2) Investigate trends in reservoir fluid flow; (3) Integrate fluid flow trends with reservoir architecture; and (4) Estimate original oil-in-place, residual oil saturation, and remaining mobile oil, to identify opportunities for reserve growth. There are three main oil-producing reservoirs in the Oficina Formation that were deposited in a bed-load fluvial system, an incised valley-fill, and a barrier-strandplain system. Reservoir continuity is complex because, in addition to lateral facies variability, the major Oficina depositional systems were internally subdivided by high-frequency stratigraphic surfaces. These surfaces define times of intermittent lacustrine and marine flooding events that punctuated the fluvial and marginal marine sedimentation, respectively. Syn and post depositional faulting further disrupted reservoir continuity. Trends in fluid flow established from initial fluid levels, response to recompletion workovers, and pressure depletion data demonstrated barriers to lateral and vertical fluid flow caused by a combination of reservoir facies pinchout, flooding shale markers, and the faults. Considerable reserve growth potential exists at Budare field because the reservoir units are highly compartment by the depositional heterogeneity and structural complexity. Numerous reserve growth opportunities were identified in attics updip of existing production, in untapped or incompletely drained compartments, and in field extensions.

  15. Uptake of donor lymphocytes treated with 8-methoxypsoralen and ultraviolet A light by recipient dendritic cells induces CD4{sup +}CD25{sup +}Foxp3{sup +} regulatory T cells and down-regulates cardiac allograft rejection

    SciTech Connect (OSTI)

    Zheng, De-Hua; Dou, Li-Ping; Wei, Yu-Xiang; Du, Guo-Sheng; Zou, Yi-Ping; Song, Ji-Yong; Zhu, Zhi-Dong; Cai, Ming; Qian, Ye-Yong; Shi, Bing-Yi

    2010-05-14

    Extracorporeal photopheresis (ECP) is an effective immunomodulatory therapy and has been demonstrated to be beneficial for graft-vs-host disease and solid-organ allograft rejection. ECP involves reinfusion of a patient's autologous peripheral blood leukocytes treated ex vivo with 8-methoxypsoralen and UVA light radiation (PUVA). Previous studies focused only on ECP treatment of recipient immune cells. Our study is the first to extend the target of ECP treatment to donor immune cells. The results of in vitro co-culture experiments demonstrate uptake of donor PUVA-treated splenic lymphocytes (PUVA-SPs) by recipient immature dendritic cells (DCs). Phagocytosis of donor PUVA-SPs does not stimulate phenotype maturation of recipient DCs. In the same co-culture system, donor PUVA-SPs enhanced production of interleukin-10 and interferon-{gamma} by recipient DCs and impaired the subsequent capability of recipient DCs to stimulate recipient naive T cells. Phagocytosis of donor PUVA-SP (PUVA-SP DCs) by recipient DCs shifted T-cell responses in favor of T helper 2 cells. Infusion of PUVA-SP DCs inhibited cardiac allograft rejection in an antigen-specific manner and induced CD4{sup +}CD25{sup high}Foxp3{sup +} regulatory T cells. In conclusion, PUVA-SP DCs simultaneously deliver the donor antigen and the regulatory signal to the transplant recipient, and thus can be used to develop a novel DC vaccine for negative immune regulation and immune tolerance induction.

  16. Simultaneous determination of thorium, niobium, lead, and zinc by photon-induced x-ray fluorescence of lateritic material

    SciTech Connect (OSTI)

    LaBrecque, J.J.; Adames, D.; Parker, W.C.

    1981-01-01

    A rapid method is presented for the simultaneous determinations of thorium, niobium, lead, and zinc in lateritic material from Cerro Impacto, Estado Bolivar, Venezuela. This technique uses a PDP - 11/05 processor - based photon induced x-ray fluorescence system. The total variations of approximately 5% for concentrations of approximately 1 and 10% for concentrations of approximately 0.1% were obtained with only 500 s of fluorescent time. The values obtained by this method were in agreement with values measured by conventional flame atomic absorption spectroscopy for lead and zinc. The values for thorium measured were in agreement with the reported values for the reference materials supplied by NBL.

  17. Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations Regulations Security Reviews Click Here FOIA Appeals Click Here Whistleblower Protection Click Here Procedural Regulations Click Here Browse Title 10 Energy Click Here

  18. Charge regulation circuit

    DOE Patents [OSTI]

    Ball, Don G. (Livermore, CA)

    1992-01-01

    A charge regulation circuit provides regulation of an unregulated voltage supply in the range of 0.01%. The charge regulation circuit is utilized in a preferred embodiment in providing regulated voltage for controlling the operation of a laser.

  19. Regulators, Requirements, Statutes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regulators, Requirements, Statutes Regulators, Requirements, Statutes The Laboratory must comply with environmental laws and regulations that apply to Laboratory operations. Contact Environmental Communication & Public Involvement P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505) 667-0216 Email Environmental laws and regulations LANL complies with more than 30 state and federal regulations and policies designed to protect human health and the environment. Regulators Regulators Environmental

  20. Multimedia regulated chemicals

    SciTech Connect (OSTI)

    Lee, C.C.; Huffman, G.L.; Mao, Y.L.

    1999-10-01

    This article examines those chemicals that are listed in either environmental laws or regulations. Its objective is to help readers determine which laws regulate what types of chemicals and which types of chemicals are regulated by what laws. It is multimedia in scope, describing the various chemicals that are regulated in the different media (i.e., air, water, or land).

  1. Experiences in the design of CRA`s for erosion/corrosion control in the production facilities of eastern Venezuela oil fields

    SciTech Connect (OSTI)

    Romero, N.; Palacios, C.A.

    1997-08-01

    It is a well known fact that CRA`s are used in the oil industry as one way to control erosion/corrosion effects. Many fields in the eastern region of Venezuela are considered corrosive due to the presence of CO{sub 2} (5 to 20%), H{sub 2}S (up to 5 ppm), and water (50% water cut) contained in the produced hydrocarbons (condensated). For some areas, the hydrocarbon is accompanied by sand, making them erosive as well. These conditions and frequent failures experienced in the field, led to the use of CRA`s. For the wells, 13% Cr and bimetallic (carbon steel/13% Cr) tubing was used for 51 condensate wells containing 5 to 20% CO{sub 2}. For the surface equipment (valves, reducers, expanders and other types of fittings) tungsten carbide hard facing were used, for some of the valves, a epoxi-phenolic coating was used. This article describes the different design criteria used for the installation of the tubing, the logistics involved during field inspections and handling tips to avoid galling during workovers. It also, presents results from the bi-metallic tubing and the hard facings used for the surface equipment.

  2. CO{sub 2} emissions from developing countries: Better understanding the role of Energy in the long term. Volume 2, Argentina, Brazil, Mexico, and Venezuela

    SciTech Connect (OSTI)

    Ketoff, A.; Sathaye, J.; Goldman, N.

    1991-07-01

    Recent years have witnessed a growing recognition of the link between emissions of carbon dioxide (CO{sub 2}) and changes in the global climate. Of all anthropogenic activities, energy production and use generate the single largest portion of these greenhouse gases. Although developing countries currently account for a small share of global carbon emissions, their contribution is increasing rapidly. Due to the rapid expansion of energy demand in these nations, the developing world`s share in global modern energy use rose from 16 to 27 percent between 1970 and 1990. If the growth rates observed over the past 20 years persist energy demand in developing will surpass that in the countries of the Organization for Economic Cooperation and Development (OECD) early in the 21st century. The study seeks to examine the forces that galvanize the growth of energy use and carbon emissions, to assess the likely future levels of energy and CO{sub 2} in selected developing nations and to identify opportunities for restraining this growth. The purpose of this report is to provide the quantitative information needed to develop effective policy options, not to identify the options themselves. These individual studies were conducted fro Argentina, Brazil, Mexico and Venezuela in Latin America.

  3. Load regulating expansion fixture

    DOE Patents [OSTI]

    Wagner, L.M.; Strum, M.J.

    1998-12-15

    A free standing self contained device for bonding ultra thin metallic films, such as 0.001 inch beryllium foils is disclosed. The device will regulate to a predetermined load for solid state bonding when heated to a bonding temperature. The device includes a load regulating feature, whereby the expansion stresses generated for bonding are regulated and self adjusting. The load regulator comprises a pair of friction isolators with a plurality of annealed copper members located therebetween. The device, with the load regulator, will adjust to and maintain a stress level needed to successfully and economically complete a leak tight bond without damaging thin foils or other delicate components. 1 fig.

  4. Load regulating expansion fixture

    DOE Patents [OSTI]

    Wagner, Lawrence M.; Strum, Michael J.

    1998-01-01

    A free standing self contained device for bonding ultra thin metallic films, such as 0.001 inch beryllium foils. The device will regulate to a predetermined load for solid state bonding when heated to a bonding temperature. The device includes a load regulating feature, whereby the expansion stresses generated for bonding are regulated and self adjusting. The load regulator comprises a pair of friction isolators with a plurality of annealed copper members located therebetween. The device, with the load regulator, will adjust to and maintain a stress level needed to successfully and economically complete a leak tight bond without damaging thin foils or other delicate components.

  5. Pressure reducing regulator

    DOE Patents [OSTI]

    Whitehead, John C. (Davis, CA); Dilgard, Lemoyne W. (Willits, CA)

    1995-01-01

    A pressure reducing regulator that controls its downstream or outlet pressure to a fixed fraction of its upstream or inlet pressure. The regulator includes a housing which may be of a titanium alloy, within which is located a seal or gasket at the outlet end which may be made of annealed copper, a rod, and piston, each of which may be made of high density graphite. The regulator is insensitive to temperature by virtue of being without a spring or gas sealed behind a diaphragm, and provides a reference for a system in which it is being used. The rod and piston of the regulator are constructed, for example, to have a 1/20 ratio such that when the downstream pressure is less than 1/20 of the upstream pressure the regulator opens and when the downstream pressure exceeds 1/20 of the upstream pressure the regulator closes.

  6. Pressure reducing regulator

    DOE Patents [OSTI]

    Whitehead, J.C.; Dilgard, L.W.

    1995-10-10

    A pressure reducing regulator that controls its downstream or outlet pressure to a fixed fraction of its upstream or inlet pressure is disclosed. The regulator includes a housing which may be of a titanium alloy, within which is located a seal or gasket at the outlet end which may be made of annealed copper, a rod, and piston, each of which may be made of high density graphite. The regulator is insensitive to temperature by virtue of being without a spring or gas sealed behind a diaphragm, and provides a reference for a system in which it is being used. The rod and piston of the regulator are constructed, for example, to have a 1/20 ratio such that when the downstream pressure is less than 1/20 of the upstream pressure the regulator opens and when the downstream pressure exceeds 1/20 of the upstream pressure the regulator closes. 10 figs.

  7. Resources for Utility Regulators

    SciTech Connect (OSTI)

    SEE Action

    2012-06-01

    Provides a summary of State and Local Energy Efficiency Action Network (SEE Action) information resources available to utility regulators, organized by topic.

  8. Policy and Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations Policy and Regulations map-1019833_960_720.jpg

  9. WIPP Documents - Federal Regulations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Federal Regulations 40 CFR Part 191 Environmental radiation protection standards for management and disposal of spent nuclear fuel, high-level and transuranic radioactive wastes. 40 CFR Part 194 Criteria for the certification and re-certification of the Waste Isolation Pilot Plant's compliance with the 40 CFR Part 191 disposal regulations.

  10. Of Refrigerators & Regulations

    Broader source: Energy.gov [DOE]

    Editor's Note: This entry has been cross-posted from The White House Blog. The President took a moment during his speech to put the debate over regulation in a different perspective.

  11. HIGH PRESSURE GAS REGULATOR

    DOE Patents [OSTI]

    Ramage, R.W.

    1962-05-01

    A gas regulator operating on the piston and feedback principle is described. The device is particularly suitable for the delicate regulation of high pressure, i.e., 10,000 psi and above, gas sources, as well as being perfectly adaptable for use on gas supplies as low as 50 psi. The piston is adjustably connected to a needle valve and the movement of the piston regulates the flow of gas from the needle valve. The gas output is obtained from the needle valve. Output pressure is sampled by a piston feedback means which, in turn, regulates the movement of the main piston. When the output is other than the desired value, the feedback system initiates movement of the main piston to allow the output pressure to be corrected or to remain constant. (AEC)

  12. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit...

  13. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Auxin Regulation Mechanism Discovered Print Wednesday, 29 August 2007 00:00 The plant hormone auxin regulates many plant growth and development processes, including shoot growth,...

  14. Reviving regulation - and antitrust

    SciTech Connect (OSTI)

    Shepherd, W.G.

    1994-06-01

    The ills of utility regulation have been overstated by interested players and at least some academics. One can have the best features of both competition and regulation by a paradigm that combines some regulation with stronger antitrust enforcement. It is now obvious that the success of deregulation actually depends crucially on whether antitrust policy is effective in preventing monopoly. In fact, antitrust policies have been extremely weak since 1980, under a deliberate Reagan administration withdrawal of enforcement. Where deregulation has led to market dominance, which antitrust cannot well cure, the policy changes of the 1980`s may have created costly and irreversible mistakes in a number of major sectors. Yet fast-paced events are currently creating a frenzy of even more deregulation, supposedly to fit new {open_quotes}revolutions{close_quotes} in technology and the impacts of large mergers. Instead it is time for particular caution in electricity.

  15. Litigation, Regulation and Enforcement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    General Counsel for Legislation, Regulation and Energy Efficiency (GC-33) Litigation, Regulation and Enforcement Litigation Enforcement Legislation, Regulation and Energy ...

  16. Categorical Exclusion Determinations: Natural Gas Regulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Natural Gas Regulation Categorical Exclusion Determinations: Natural Gas Regulation Categorical Exclusion Determinations issued by Natural Gas Regulation. DOCUMENTS AVAILABLE FOR ...

  17. Regulation of Meiotic Recombination

    SciTech Connect (OSTI)

    Gregory p. Copenhaver

    2011-11-09

    Meiotic recombination results in the heritable rearrangement of DNA, primarily through reciprocal exchange between homologous chromosome or gene conversion. In plants these events are critical for ensuring proper chromosome segregation, facilitating DNA repair and providing a basis for genetic diversity. Understanding this fundamental biological mechanism will directly facilitate trait mapping, conventional plant breeding, and development of genetic engineering techniques that will help support the responsible production and conversion of renewable resources for fuels, chemicals, and the conservation of energy (1-3). Substantial progress has been made in understanding the basal recombination machinery, much of which is conserved in organisms as diverse as yeast, plants and mammals (4, 5). Significantly less is known about the factors that regulate how often and where that basal machinery acts on higher eukaryotic chromosomes. One important mechanism for regulating the frequency and distribution of meiotic recombination is crossover interference - or the ability of one recombination event to influence nearby events. The MUS81 gene is thought to play an important role in regulating the influence of interference on crossing over. The immediate goals of this project are to use reverse genetics to identify mutants in two putative MUS81 homologs in the model plant Arabidopsis thaliana, characterize those mutants and initiate a novel forward genetic screen for additional regulators of meiotic recombination. The long-term goal of the project is to understand how meiotic recombination is regulated in higher eukaryotes with an emphasis on the molecular basis of crossover interference. The ability to monitor recombination in all four meiotic products (tetrad analysis) has been a powerful tool in the arsenal of yeast geneticists. Previously, the qrt mutant of Arabidopsis, which causes the four pollen products of male meiosis to remain attached, was developed as a facile system for assaying recombination using tetrad analysis in a higher eukaryotic system (6). This system enabled the measurement of the frequency and distribution of recombination events at a genome wide level in wild type Arabidopsis (7), construction of genetic linkage maps which include positions for each centromere (8), and modeling of the strength and pattern of interference (9). This proposal extends the use of tetrad analysis in Arabidopsis by using it as the basis for assessing the phenotypes of mutants in genes important for recombination and the regulation of crossover interference and performing a novel genetic screen. In addition to broadening our knowledge of a classic genetic problem - the regulation of recombination by crossover interference - this proposal also provides broader impact by: generating pedagogical tools for use in hands-on classroom experience with genetics, building interdisciplinary collegial partnerships, and creating a platform for participation by junior scientists from underrepresented groups. There are three specific aims: (1) Isolate mutants in Arabidopsis MUS81 homologs using T-DNA and TILLING (2) Characterize recombination levels and interference in mus81 mutants (3) Execute a novel genetic screen, based on tetrad analysis, for genes that regulate meiotic recombination

  18. ELECTRON EMISSION REGULATING MEANS

    DOE Patents [OSTI]

    Brenholdt, I.R.

    1957-11-19

    >An electronic regulating system is described for controlling the electron emission of a cathode, for example, the cathode in a mass spectrometer. The system incorporates a transformer having a first secondary winding for the above-mentioned cathode and a second secondary winding for the above-mentioned cathode and a second secondary winding load by grid controlled vacuum tubes. A portion of the electron current emitted by the cathode is passed through a network which develops a feedback signal. The system arrangement is completed by using the feedback signal to control the vacuum tubes in the second secondary winding through a regulator tube. When a change in cathode emission occurs, the feedback signal acts to correct this change by adjusting the load on the transformer.

  19. Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems

    Broader source: Energy.gov [DOE]

    This Protocol is a living guidance document for geothermal developers, public officials, regulators and the general public that provides a set of general guidelines detailing useful steps to evaluate and manage the effects of induced seismicity related to EGS projects.

  20. Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems

    SciTech Connect (OSTI)

    Majer, Ernie; Nelson, James; Robertson-Tait, Ann; Savy, Jean; Wong, Ivan

    2012-01-01

    This Protocol is a living guidance document for geothermal developers, public officials, regulators and the general public that provides a set of general guidelines detailing useful steps to evaluate and manage the effects of induced seismicity related to EGS projects.

  1. Interrelationship of Program Regulations and Financial Assistance Regulations

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1980-04-24

    The purpose of this Order is to set forth the interrelationship between all program regulations which will result in assistance awards (Program Regulations) and the Department of Energy Assistance Regulations (DOE-AR, 10 CRF Part 600), including procedures for exceptions, deviations or waivers.

  2. Isolated yeast promoter sequence and a method of regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway; Skeen, Rodney S.; Hooker, Brian S.; Anderson, Daniel B.

    2005-05-31

    The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  3. Self-regulating valve

    DOE Patents [OSTI]

    Humphreys, D.A.

    1982-07-20

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  4. Temperature controlled high voltage regulator

    DOE Patents [OSTI]

    Chiaro, Jr., Peter J. (Clinton, TN); Schulze, Gerald K. (Knoxville, TN)

    2004-04-20

    A temperature controlled high voltage regulator for automatically adjusting the high voltage applied to a radiation detector is described. The regulator is a solid state device that is independent of the attached radiation detector, enabling the regulator to be used by various models of radiation detectors, such as gas flow proportional radiation detectors.

  5. Cascaded'' pilot regulators help reduce LPG loss in hot weather

    SciTech Connect (OSTI)

    Not Available

    1994-08-08

    Fina Oil and Chemical Co. and Fisher Controls International used engineering resourcefulness to overcome heat-induced product loss from LPG storage bullets at Fina's Port Arthur, Tex., refinery. Fina had installed Fisher's Easy Joe 399A-6365, a pilot-operated, back-pressure-type regulator, on its LPG storage facility in 1991 as part of a fuel products modernization project. The regulators helped control the accumulation of noncondensible vapors, which collect in the storage bullets above the LPG. But summer heat extremes and surges in the tanks and lines made it possible for the operating pressure to increase so that the safety relief valve was activated before the pilot regulator was able to stabilize the pressure. The installation of pilot-type regulators, in cascaded, or series, formation, reduced product venting through relief valves.

  6. Effects of acteoside on lipopolysaccharide-induced inflammation in acute

    Office of Scientific and Technical Information (OSTI)

    lung injury via regulation of NF-κB pathway in vivo and in vitro (Journal Article) | SciTech Connect Effects of acteoside on lipopolysaccharide-induced inflammation in acute lung injury via regulation of NF-κB pathway in vivo and in vitro Citation Details In-Document Search Title: Effects of acteoside on lipopolysaccharide-induced inflammation in acute lung injury via regulation of NF-κB pathway in vivo and in vitro The purpose of the present study was to investigate the protective role

  7. Vermont Hazardous Waste Management Regulations | Open Energy...

    Open Energy Info (EERE)

    Hazardous Waste Management Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Vermont Hazardous Waste Management...

  8. Use of geostatistic techniques to describe a reservoir to be submitted into a secondary recovery process field case: {open_quotes}Eocene B-Inferior/VLG-3659, Ceuta, Venezuela{close_quotes}

    SciTech Connect (OSTI)

    Hernandez, T.; Poquioma, W.

    1997-08-01

    This study presents the results of an integrated reservoir study of the Eocene B-Inferior/VLG-3659, Area 7, Ceuta filed. This field located in the Maracaibo Lake in the western side of Venezuela. The objective was to evaluating the feasibility to implement a secondary recovery project by means of water flooding. Core information was used for this study (194 ft), PVT analysis, RFI, build-up and statistic`s pressure analysis, modem logs and production history data. Using geostatistical techniques (Kriging) it was defined a low uncertainty geological model that was validated by means of a black oil simulator (Eclipse). The results showed a good comparison of historical pressure of the reservoir against those obtained from the model, without the need of {open_quotes}history matching{close_quotes}. It means without modifying neither the initial rock properties nor reservoir fluids. The results of this study recommended drilling in two new locations, also the reactivation of four producing wells and water flooding under peripherical array by means of four injection wells, with the recovery of an additional 30.2 MMSTB. The economical evaluation shows an internal return rate of 31.4%.

  9. Regulators, Boards, Councils - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    About Hanford Cleanup Regulators, Boards, Councils About Us About Hanford Cleanup Regulators, Boards, Councils Hanford Advisory Board Hanford Natural Resource Trustee Council Environmental Protection Agency Washington State Department of Ecology Defense Nuclear Facilities Safety Board Hanford History Hanford Site Wide Programs Contact Us Regulators, Boards, Councils Email Email Page | Print Print Page |Text Increase Font Size Decrease Font Size There are numerous entities involved in Hanford

  10. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Unique Auxin Regulation Mechanism Discovered Print Wednesday, 29 August 2007 00:00 The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction

  11. Collegiate Wind Competition Rules and Regulations | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Collegiate Wind Competition Rules and Regulations Collegiate Wind Competition Rules and Regulations The competition challenges interdisciplinary teams of undergraduate students ...

  12. Street sweeping and stormwater regulations

    SciTech Connect (OSTI)

    Not Available

    1993-10-01

    This article examines the role of street sweeping in meeting the requirements of the Clean Water Act stormwater regulations. The article identifies those industrial and municipal activities which are covered by the regulations and cites frequent sweeping of site surfaces for industry and street sweeping for municipalities as an integral part of compliance plans.

  13. Insight in the multilevel regulation of NER

    SciTech Connect (OSTI)

    Dijk, Madelon; Typas, Dimitris; Mullenders, Leon; Pines, Alex

    2014-11-15

    Nucleotide excision repair (NER) is a key component of the DNA damage response (DDR) and it is essential to safeguard genome integrity against genotoxic insults. The regulation of NER is primarily mediated by protein post-translational modifications (PTMs). The NER machinery removes a wide spectrum of DNA helix distorting lesions, including those induced by solar radiation, through two sub-pathways: global genome nucleotide excision repair (GG-NER) and transcription coupled nucleotide excision repair (TC-NER). Severe clinical consequences associated with inherited NER defects, including premature ageing, neurodegeneration and extreme cancer-susceptibility, underscore the biological relevance of NER. In the last two decades most of the core NER machinery has been elaborately described, shifting attention to molecular mechanisms that either facilitate NER in the context of chromatin or promote the timely and accurate interplay between NER factors and various post-translational modifications. In this review, we summarize and discuss the latest findings in NER. In particular, we focus on emerging factors and novel molecular mechanisms by which NER is regulated.

  14. Alternative Fuels Data Center: Conversion Regulations

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Conversion Regulations to someone by E-mail Share Alternative Fuels Data Center: Conversion Regulations on Facebook Tweet about Alternative Fuels Data Center: Conversion Regulations on Twitter Bookmark Alternative Fuels Data Center: Conversion Regulations on Google Bookmark Alternative Fuels Data Center: Conversion Regulations on Delicious Rank Alternative Fuels Data Center: Conversion Regulations on Digg Find More places to share Alternative Fuels Data Center: Conversion Regulations on

  15. APPARATUS FOR REGULATING HIGH VOLTAGE

    DOE Patents [OSTI]

    Morrison, K.G.

    1951-03-20

    This patent describes a high-voltage regulator of the r-f type wherein the modulation of the r-f voltage is accomplished at a high level, resulting in good stabilization over a large range of load conditions.

  16. Environmental Regulations | Open Energy Information

    Open Energy Info (EERE)

    Regulations Jump to: navigation, search To do: add table This article is a stub. You can help OpenEI by expanding it. Retrieved from "http:en.openei.orgwindex.php?titleEnviron...

  17. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  18. Federal Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Federal Regulations Federal Regulations keyboard-621831_960_720.jpg Cybersecurity Atomic Energy Act of 1954 (pdf) Computer Fraud and Abuse Act of 1986 Electronic Communications Privacy Act of 1986 Federal Information Security Management Act of 2002 (Title III of E-Gov) Homeland Security Act of 2002 (includes Cyber Security Act of 2002 and Critical Infrastructure Act of 2002) Uniting and Strengthening America by Providing Appropriate Tools Required to Intercept and Obstruct Terrorism Act Cyber

  19. Federal Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Federal Regulations Federal Regulations NOTE: Adobe Acrobat Reader may be necessary to view PDF documents listed below. Accessibility Section 508 of the Rehabilitation Act - Electronic and Information Technology Accessibility Standards Section 504 of the Rehabilitation Act - Nondiscrimination Under Federal Grants and Programs Federal Records Act Freedom of Information Act (as amended) Freedom of Information Act Updates Privacy Act of 1974 (as amended) Privacy Act Overview Cyber Security Atomic

  20. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  1. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  2. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  3. Post regulation circuit with energy storage

    DOE Patents [OSTI]

    Ball, Don G.; Birx, Daniel L.; Cook, Edward G.

    1992-01-01

    A charge regulation circuit provides regulation of an unregulated voltage supply and provides energy storage. The charge regulation circuit according to the present invention provides energy storage without unnecessary dissipation of energy through a resistor as in prior art approaches.

  4. Output-Based Regulations: A Handbook for Air Regulators (U.S...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004 Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004 The U.S. ...

  5. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, Charlton; Subbaraman, Maria R.

    1989-01-01

    An improvement in an inducer for a pump wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs.

  6. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, C.; Subbaraman, M.R.

    1989-06-13

    An improvement in an inducer for a pump is disclosed wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs. 11 figs.

  7. Regulations, Guidelines and Codes and Standards | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Safety, Codes & Standards Current Approaches to Safety, Codes & Standards Regulations, Guidelines and Codes and Standards Regulations, Guidelines and Codes and Standards Many ...

  8. Alaska ADEC Wetlands Regulation | Open Energy Information

    Open Energy Info (EERE)

    Wetlands Regulation Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Alaska ADEC Wetlands Regulation Author Alaska Division of Water Published Alaska...

  9. Fish Habitat Regulations | Open Energy Information

    Open Energy Info (EERE)

    Fish Habitat Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Fish Habitat Regulations Author Alaska Department of Fish & Game Published...

  10. International Electricity Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation International Electricity Regulation U.S. trade in electric energy with Canada and Mexico is rising, bringing economic and reliability benefits to the United States and ...

  11. ISOs: The new antitrust regulators?

    SciTech Connect (OSTI)

    Raskin, D.B.

    1998-04-01

    Fear of seller market power in emerging electricity markets has led regulators to sanction use of independent system operators as private market police. A more restrained approach is likely to yield better results without the chilling effects of private regulation. This new industry regulatory paradigm has received little critical attention to date. This is unfortunate because ISO antitrust regulation raises serious legal and policy concerns. The California and New England Power Pool (NEPOOL) plans are quite intrusive. They require the ISO to make difficult distinctions between acceptable and unacceptable market behavior. They create considerable risk that desirable competitive behavior will be chilled and that market participants will incur significant explicit and implicit costs to meet regulatory requirements.

  12. Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems (EGS)

    Broader source: Energy.gov [DOE]

    This document is intended to assist industry and regulators identify important issues and parameters that may be necessary for the evaluation and mitigation of adverse effects of induced seismicity.

  13. Chapter 2 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    2 of the Wyoming Public Service Commission Regulations: General Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  14. Frequency regulator for synchronous generators

    DOE Patents [OSTI]

    Karlicek, Robert F.

    1982-01-01

    The present invention is directed to a novel frequency regulator which controls a generator output frequency for variations in both the input power to the generator and the power supplied to an uncontrolled external load. The present invention further includes over current and current balance protection devices which are relatively inexpensive to manufacture, which may be encapsulated to provide protection from the operating environment and which respond more quickly than previously known electromechanical devices.

  15. Photomultiplier tube gain regulating system

    DOE Patents [OSTI]

    Johnson, Wayne F.

    1976-01-01

    This invention relates to an improved system for regulating the gain of a photomultiplier tube, and was designed for use with the photomultiplier tubes of a GeMSAEC fast analyzers. It has the following advantages over the prior system: noise is virtually eliminated; sample analysis can begin after 3 to 4 revolutions of the rotor; fluorescent and light scattering solutions can be used as a reference; and the reference solution can be in any cuvette on the rotor.

  16. GP-8 LAWS AND REGULATIONS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    40-DOP (5-2011) Supersedes (7-2010) issue DECLARATION of OCCUPATIONAL MEDICINE PROVIDER GENERAL INFORMATION PURPOSE All Contractors and their lower-tier subcontractors must comply with the Department of Energy's Worker Safety and Health Program regulation, 10 CFR 851, "Worker Safety and Health Program (WSHP). The WSHP enforces worker safety and health requirements including, but not limited to, standards of the Occupational Safety and Health Administration (OSHA), American National

  17. Frequency regulator for synchronous generators

    DOE Patents [OSTI]

    Karlicek, R.F.

    1982-08-10

    The present invention is directed to a novel frequency regulator which controls a generator output frequency for variations in both the input power to the generator and the power supplied to an uncontrolled external load. The present invention further includes over current and current balance protection devices which are relatively inexpensive to manufacture, which may be encapsulated to provide protection from the operating environment and which respond more quickly than previously known electromechanical devices. 11 figs.

  18. NRC - regulator of nuclear safety

    SciTech Connect (OSTI)

    1997-05-01

    The U.S. Nuclear Regulatory Commission (NRC) was formed in 1975 to regulate the various commercial and institutional uses of nuclear energy, including nuclear power plants. The agency succeeded the Atomic Energy Commission, which previously had responsibility for both developing and regulating nuclear activities. Federal research and development work for all energy sources, as well as nuclear weapons production, is now conducted by the U.S. Department of Energy. Under its responsibility to protect public health and safety, the NRC has three principal regulatory functions: (1) establish standards and regulations, (2) issue licenses for nuclear facilities and users of nuclear materials, and (3) inspect facilities and users of nuclear materials to ensure compliance with the requirements. These regulatory functions relate to both nuclear power plants and to other uses of nuclear materials - like nuclear medicine programs at hospitals, academic activities at educational institutions, research work, and such industrial applications as gauges and testing equipment. The NRC places a high priority on keeping the public informed of its work. The agency recognizes the interest of citizens in what it does through such activities as maintaining public document rooms across the country and holding public hearings, public meetings in local areas, and discussions with individuals and organizations.

  19. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway [Richland, WA; Skeen, Rodney S [Pendleton, OR; Hooker, Brian S [Kennewick, WA; Anderson, Daniel B [Pasco, WA

    2003-04-01

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  20. Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004

    Broader source: Energy.gov [DOE]

    Handbook providing practical information to help regulators decide if they want to use output-based regulations and explains how to develop an output-based emission standard

  1. Colorado Code of Regulations 4 CCR 723-3, Rules Regulating Electric...

    Open Energy Info (EERE)

    Colorado Code of Regulations 4 CCR 723-3, Rules Regulating Electric Utilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  2. Code of Colorado Regulations 4 CCR 723-3, Rules Regulating Electric...

    Open Energy Info (EERE)

    Code of Colorado Regulations 4 CCR 723-3, Rules Regulating Electric Utilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  3. Surface mine regulations complicate reclamation

    SciTech Connect (OSTI)

    Seltz-Patrash, A.

    1980-09-01

    The Surface Mining Control and Reclamation Act of 1977 is a landmark environmental law intended to protect U.S. lands from stripmining effects. However, coal mine operators claim that some SMCRA regulations are misguidedcosting time and money, but yielding no substantial environmental benefit. Unlike other environmental acts, SMCRA details specifically the goals of reclamation and the methods that must be implemented to meet these goals. Coal industry representatives believe that this discourages innovation, promotes inefficiency by ignoring regional differences among sites, and results in unnecessary expense to the industry. Reclamation practices and progress among western coal mining companies are evaluated. (1 map, 5 photos)

  4. Clean Water Act and Regulations (EPA)

    Broader source: Energy.gov [DOE]

    The Clean Water Act (CWA; 33 U.S.C. §1251 et seq.) establishes the basic structure for regulating discharges of pollutants into the waters of the United States and regulating quality standards for surface waters.

  5. Investment and regulation: the Dutch experience

    SciTech Connect (OSTI)

    Haffner, Robert; Helmer, Dorine; van Til, Harry

    2010-06-15

    Theoretical studies on the relationship between incentive regulation and investment in network industries generally point out that incentive regulation has a negative impact on investment. However, empirical evidence in this area is scarce. An analysis suggests that in the Dutch electricity and gas networks since 2001, incentive regulation has ensured a more rational and professional approach towards investments, with investment levels coming down somewhat at the start of the regulation but picking up later on. (author)

  6. certification, compliance and enforcement regulations for Commercial...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) More Documents & Publications Regulatory Burden RFI Executive Order ...

  7. Regulations for beneficial use changing nationwide

    SciTech Connect (OSTI)

    Goss, D.

    2008-07-01

    Regulations on the use of coal combustion products (CCPs) that are being considered by various US states are reviewed.

  8. Environmental Laws and Regulations | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Record Files Natural Resource Damage Assessments Environmental Laws and Regulations More Documents & Publications Paducah Public Involvement Plan Portsmouth Removal Actions...

  9. certification, compliance and enforcement regulations for Commercial

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Refrigeration Equipment (CRE) | Department of Energy certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) The current certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) "simply makes no sense". The regulations define the basic model as any product that has a different energy use or efficiency

  10. Reciprocal regulation of LXRα activity by ASXL1 and ASXL2 in lipogenesis

    SciTech Connect (OSTI)

    Park, Ui-Hyun; Seong, Mi-ran; Kim, Eun-Joo; Hur, Wonhee; Kim, Sung Woo; Yoon, Seung Kew; Um, Soo-Jong

    2014-01-10

    Highlights: •ASXL1 and ASXL2 directly interact with ligand-bound LXRα. •Ligand-induced LXRα activity is repressed by ASXL1 and activated by ASXL2. •ASXL1 and ASXL2 bind to the LXRE of the LXRα target promoter. •ASXL1 and ASXL2 reciprocally regulate lipogenesis in liver cells. -- Abstract: Liver X receptor alpha (LXRα), a member of the nuclear receptor superfamily, plays a pivotal role in hepatic cholesterol and lipid metabolism, regulating the expression of genes associated with hepatic lipogenesis. The additional sex comb-like (ASXL) family was postulated to regulate chromatin function. Here, we investigate the roles of ASXL1 and ASXL2 in regulating LXRα activity. We found that ASXL1 suppressed ligand-induced LXRα transcriptional activity, whereas ASXL2 increased LXRα activity through direct interaction in the presence of the ligand. Chromatin immunoprecipitation (ChIP) assays showed ligand-dependent recruitment of ASXLs to ABCA1 promoters, like LXRα. Knockdown studies indicated that ASXL1 inhibits, while ASXL2 increases, lipid accumulation in H4IIE cells, similar to their roles in transcriptional regulation. We also found that ASXL1 expression increases under fasting conditions, and decreases in insulin-treated H4IIE cells and the livers of high-fat diet-fed mice. Overall, these results support the reciprocal role of the ASXL family in lipid homeostasis through the opposite regulation of LXRα.

  11. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Twitter Google + Vimeo GovDelivery SlideShare Laser-induced incandescence (LII) HomeClimate & Earth SystemsClimate Measurement & ModelingLaser-induced incandescence (LII) ...

  12. Venezuela: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    in the country has fallen, while domestic consumption has risen, causing a decline in net oil exports. EIA estimates the Venezuelan net exports fell again in 2010 to 1.59...

  13. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  14. Physiology and Regulation of Calcium Channels in Stomatal Guard Cells

    SciTech Connect (OSTI)

    Schroeder, Julian I.

    2007-05-02

    Stomatal pores in the epidermis of leaves regulate the diffusion of CO2 into leaves for photosynthetic carbon fixation and control water loss of plants during drought periods. Guard cells sense CO2, water status, light and other environmental conditions to regulate stomatal apertures for optimization of CO2 intake and plant growth under drought stress. The cytosolic second messenger calcium contributes to stomatal movements by transducing signals and regulating ion channels in guard cells. Studies suggest that both plasma membrane Ca2+ influx channels and vacuolar/organellar Ca2+ release channels contribute to ABA-induced Ca2+ elevations in guard cells. Recent research in the P.I.'s laboratory has led to identification of a novel major cation-selective Ca2+-permeable influx channel (Ica) in the plasma membrane of Arabidopsis guard cells. These advances will allow detailed characterization of Ica plasma membrane Ca2+ influx channels in guard cells. The long term goal of this research project is to gain a first detailed characterization of these novel plasma membrane Ca2+-permeable channel currents in Arabidopsis guard cells. The proposed research will investigate the hypothesis that Ica represents an important Ca2+ influx pathway for ABA and CO2 signal transduction in Arabidopsis guard cells. These studies will lead to elucidation of key signal transduction mechanisms by which plants balance CO2 influx into leaves and transpirational water loss and may contribute to future strategies for manipulating gas exchange for improved growth of crop plants and for biomass production.

  15. Final Report for Regulation of Embryonic Development in Higher Plants

    SciTech Connect (OSTI)

    Harada, John J.

    2013-10-22

    The overall goal of the project was to define the cellular processes that underlie embryo development in plants at a mechanistic level. Our studies focused on a critical transcriptional regulator, Arabidopsis LEAFY COTYLEDON (LEC1), that is necessary and sufficient to induce processes required for embryo development. Because LEC1 regulates lipid accumulation during the maturation phase of embryo development, information about LEC1 may be useful in designing approaches to enhance biofuel production in plants. During the tenure of this project, we determined the molecular mechanisms by which LEC1 acts as a transcription factor in embryos. We also identified genes directly regulated by LEC1 and showed that many of these genes are involved in maturation processes. This information has been useful in dissecting the gene regulatory networks controlling embryo development. Finally, LEC1 is a novel isoform of a transcription factor that is conserved among eukaryotes, and LEC1 is active primarily in seeds. Therefore, we determined that the LEC1-type transcription factors first appeared in lycophytes during land plant evolution. Together, this study provides basic information that has implications for biofuel production.

  16. Staff Listing - Office of Regulation and International Engagement, Division

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Natural Gas Regulation, Division of International Engagement | Department of Energy Staff Listing - Office of Regulation and International Engagement, Division of Natural Gas Regulation, Division of International Engagement Staff Listing - Office of Regulation and International Engagement, Division of Natural Gas Regulation, Division of International Engagement Office of Regulation and International Engagement Mailing Address: Office of Regulation and International Engagement Office of

  17. 18 CFR 380 Regulations Implementing the National Environmental...

    Open Energy Info (EERE)

    380 Regulations Implementing the National Environmental Policy Act Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 18 CFR...

  18. Chapter 9 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    9 of the Wyoming Public Service Commission Regulations: General Forms Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  19. Key Publications - Natural Gas Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Services Natural Gas Regulation Key Publications - Natural Gas Regulation Key Publications - Natural Gas Regulation Natural Gas Imports and Exports - Quarterly Reports May ...

  20. Title 27 California Code of Regulations: Environmental Protection...

    Open Energy Info (EERE)

    7 California Code of Regulations: Environmental Protection Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Title 27...

  1. Staff Listing - Office of Regulation and International Engagement...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Staff Listing - Office of Regulation and International Engagement, Division of Natural Gas Regulation, Division of International Engagement Staff Listing - Office of Regulation and ...

  2. Rules and Regulations of the Wyoming Industrial Siting Council...

    Open Energy Info (EERE)

    Document- RegulationRegulation: Rules and Regulations of the Wyoming Industrial Siting Council - Chapter 1Legal Abstract Industrial development information and siting rules and...

  3. Advisory Council on Historic Preservation - Section 106 Regulations...

    Open Energy Info (EERE)

    OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Advisory Council on Historic Preservation - Section 106 RegulationsLegal Abstract Section 106 requires...

  4. Rules and Regulations of the Wyoming Industrial Siting Council...

    Open Energy Info (EERE)

    Document- RegulationRegulation: Rules and Regulations of the Wyoming Industrial Siting Council - Chapter 2Legal Abstract Rules of practice and proceedures of the Industrial Siting...

  5. Rules and Regulations of the Industrial Siting Council, Chapter...

    Open Energy Info (EERE)

    Industrial Siting Council, Chapter 1 Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Rules and Regulations of the...

  6. WAC - 173-303 Dangerous Waste Regulations | Open Energy Information

    Open Energy Info (EERE)

    03 Dangerous Waste Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 173-303 Dangerous Waste...

  7. 2011 Air Quality Regulations Report | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Air Quality Regulations Report 2011 Air Quality Regulations Report PDF icon 2011 Air Quality Regulations Report120111.pdf More Documents & Publications 2011:...

  8. 2011: Air Quality Regulations Report | Department of Energy

    Office of Environmental Management (EM)

    : Air Quality Regulations Report 2011: Air Quality Regulations Report PDF icon 2011 Air Quality Regulations ReportA120911.pdf More Documents & Publications 2011...

  9. Regulations for Implementing the Procedural Provisions of NEPA...

    Energy Savers [EERE]

    Regulations for Implementing the Procedural Provisions of NEPA Regulations for Implementing the Procedural Provisions of NEPA PDF icon CEQ Regulations for Implementing the...

  10. Homeobox A7 stimulates breast cancer cell proliferation by up-regulating estrogen receptor-alpha

    SciTech Connect (OSTI)

    Zhang, Yu; Department of Obstetrics and Gynaecology, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4 ; Cheng, Jung-Chien; Huang, He-Feng; Leung, Peter C.K.

    2013-11-01

    Highlights: HOXA7 regulates MCF7 cell proliferation. HOXA7 up-regulates ER? expression. HOXA7 mediates estrogen-induced MCF7 cell proliferation. -- Abstract: Breast cancer is the most common hormone-dependent malignancy in women. Homeobox (HOX) transcription factors regulate many cellular functions, including cell migration, proliferation and differentiation. The aberrant expression of HOX genes has been reported to be associated with human reproductive cancers. Estradiol (E2) and its nuclear receptors, estrogen receptor (ER)-alpha and ER-beta, are known to play critical roles in the regulation of breast cancer cell growth. However, an understanding of the potential relationship between HOXA7 and ER in breast cancer cells is limited. In this study, our results demonstrate that knockdown of HOXA7 in MCF7 cells significantly decreased cell proliferation and ER? expression. In addition, HOXA7 knockdown attenuated E2-induced cell proliferation as well as progesterone receptor (PR) expression. The stimulatory effects of E2 on cell proliferation and PR expression were abolished by co-treatment with ICI 182780, a selective ER? antagonist. In contrast, overexpression of HOXA7 significantly stimulated cell proliferation and ER? expression. Moreover, E2-induced cell proliferation, as well as PR expression, was enhanced by the overexpression of HOXA7. Neither knockdown nor overexpression of HOXA7 affected the ER-beta levels. Our results demonstrate a novel mechanistic role for HOXA7 in modulating breast cancer cell proliferation via regulation of ER? expression. This finding contributes to our understanding of the role HOXA7 plays in regulating the proliferation of ER-positive cancer cells.

  11. EPA Regulation Compliance | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EPA Regulation Compliance EPA Regulation Compliance OE offers technical assistance on implementing the new and pending EPA air rules affecting the electric utility industry. Examples of typical assistance include technical information on cost and performance of the various power plant pollution retrofit control technologies; technical information on generation, demand-side or transmission alternatives for any replacement power needed for retiring generating units; and assistance to regulators

  12. Small Self-Regulating Fission Reactor System

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    359 This document is approved for public release; further dissemination unlimited Small Self-Regulating Fission Reactor System ANTICIPATED IMPACT PATH FORWARD DESCRIPTION BACKGROUND & MOTIVATION INNOVATION A power system for special government applications Point of Contact: Patrick McClure, NEN-5, pmcclure@lanl.gov (505)667-9534 Small Self-Regulating Fission Reactor System A small self- regulating fission reactor made with U 235 . LANL and NASA with the support of NSTec performed a proof of

  13. WYDOT - Utility Accommodation Regulation Manual | Open Energy...

    Open Energy Info (EERE)

    Regulation ManualPermittingRegulatory GuidanceGuideHandbook Abstract This manual sets forth the processes and procedures for utility accommodation on WYDOT rights-of-way. Author...

  14. Council on Environmental Quality - Regulations for Implementing...

    Open Energy Info (EERE)

    Implementing the Procedural Provisions of the NEPALegal Abstract These regulations set forth the processes and procedures of the National Environmental Policy Act. Published NA...

  15. Exporting licensing regulations affecting US geothermal firms

    SciTech Connect (OSTI)

    Not Available

    1988-08-01

    This document presents a brief introduction and overview of the Department of Commerce's Export Administration Regulations which might affect potential US geothermal goods exporters. It is intended to make US geothermal firms officials aware of the existence of such regulations and to provide them with references, contacts and phone numbers where they can obtain specific and detailed information and assistance. It must be stressed however, that the ultimate responsibility for complying with the above mentioned regulations lies with the exporter who must consult the complete version of the regulations.

  16. ACHP - Section 106 Regulations Flowchart Explanatory Material...

    Open Energy Info (EERE)

    Explanatory Material Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: ACHP - Section 106 Regulations Flowchart Explanatory Material Abstract This...

  17. EPA Regulations Overview Website | Open Energy Information

    Open Energy Info (EERE)

    Overview Website Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: EPA Regulations Overview Website Abstract This website contains information about...

  18. Alaska Special Area Regulations | Open Energy Information

    Open Energy Info (EERE)

    to library Web Site: Alaska Special Area Regulations Author Alaska Department of Fish & Game Published Publisher Not Provided, 2014 DOI Not Provided Check for DOI...

  19. Texas Railroad Commission - Pollution Discharge Regulations ...

    Open Energy Info (EERE)

    Not Provided DOI Not Provided Check for DOI availability: http:crossref.org Online Internet link for Texas Railroad Commission - Pollution Discharge Regulations Citation...

  20. Controlling Wind Turbines for Secondary Frequency Regulation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Controlling Wind Turbines for Secondary Frequency Regulation: An Analysis of AGC ... Workshop on Large-Scale Integration of Wind Power Into Power Systems as Well as on ...

  1. Volume regulation by human lymphocytes. Role of calcium

    SciTech Connect (OSTI)

    Grinstein, S.; Dupre, A.; Rothstein, A.

    1982-05-01

    Human peripheral blood lymphocytes regulate their volumes in hypotonic solutions. In hypotonic media in which Na+ is the predominant cation, an initial swelling phase is followed by a regulatory volume decrease (RVD) associated with a net loss of cellular K+. In media in which K+ is the predominant cation, the rapid initial swelling is followed by a slower second swelling phase. /sup 86/Rb+ fluxes increased during RVD and returned to normal when the original volume was approximately regained. Effects similar to those induced by hypotonic stress could also be produced by raising the intracellular Ca++ level. In isotonic, Ca++-containing media cells were found to shrink upon addition of the Ca++ ionophore A23187 in K+-free media, but to swell in K+-rich media. Exposure to Ca++ plus A23187 also increased /sup 86/Rb+ fluxes. Quinine (75 microM), an inhibitor of the Ca++-activated K+ pathway in other systems blocked RVD, the associated K+ loss, and the increase in /sup 86/Rb+ efflux. Quinine also inhibited the volume changes and the increased /sup 86/Rb fluxes induced by Ca++ plus ionophore. The calmodulin inhibitors trifluoperazine, pimozide and chlorpromazine blocked RVD as well as Ca++ plus A23187-induced volume changes. Trifluoperazine also prevented the increase in /sup 86/Rb+ fluxes and K+ loss induced by hypotonicity. Chlorpromazine sulfoxide, a relatively ineffective calmodulin antagonist, was considerably less potent as an inhibitor of RVD than chlorpromazine. It is suggested than an elevation in cytoplasmic (Ca++), triggered by cell swelling, increases the plasma membrane permeability to K+, the ensuing increased efflux of K+, associated anions, and osmotically obliged water, leading to cell shrinking (RVD).

  2. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, Sen Y.

    1989-01-01

    An improvement in a pump including a shrouded inducer, the improvement comprising first and second sealing means 32,36 which cooperate with a first vortex cell 38 and a series of secondary vortex cells 40 to remove any tangential velocity components from the recirculation flow.

  3. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, S.Y.

    1989-08-08

    An improvement in a pump is described including a shrouded inducer, the improvement comprising first and second sealing means which cooperate with a first vortex cell and a series of secondary vortex cells to remove any tangential velocity components from the recirculation flow. 3 figs.

  4. 5 CCR 1002-62 Colorado Regulations for Effluent Limitations ...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 5 CCR 1002-62 Colorado Regulations for Effluent LimitationsLegal Abstract Regulations...

  5. UAC R307 - Air Quality Regulations | Open Energy Information

    Open Energy Info (EERE)

    07 - Air Quality Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: UAC R307 - Air Quality RegulationsLegal...

  6. Confusion reigns over industrial stormwater regulations

    SciTech Connect (OSTI)

    Bishop, J.

    1993-01-01

    More than five years after Congress mandated controls for industrial and municipal stormwater discharges, many in the regulated community remain unclear about how the rules apply to them. The regulations' progress since the 1987 CWA amendments opened the door for their development often has been shaky and marked by setbacks. According to a federal appeals court decision issued last summer, that struggle is likely to continue. Although the original stormwater rules issued by EPA in November 1990 appeared to focus on heavy manufacturing facilities, as well as municipalities, the appellate court cleared a path that could draw light industrial plants and small construction sites within the regulations' domain. The rules in dispute bring under their umbrella any stormwater discharges associated with industrial activity to surface waters or municipal storm sewer systems. However, the regulations distinguish between facilities engaged in heavy industry and light, or enclosed industrial facilities, based on the probability that stormwater discharges will be contaminated and require regulation.

  7. Laser-Induced Fluorescence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Induced Fluorescence - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs Advanced

  8. Rainwater harvesting state regulations and technical resources

    SciTech Connect (OSTI)

    Loper, Susan A.

    2015-06-01

    Pacific Northwest National Laboratory (PNNL) conducted in-depth research of state-level rainwater harvesting regulations for the Federal Energy Management Program (FEMP) to help federal agencies strategically identify locations conducive to rainwater harvesting projects. Currently, rainwater harvesting is not regulated by the federal government but rather it is up to individual states to regulate the collection and use of rainwater. There is no centralized information on state-level regulations on rainwater harvesting maintained by a federal agency or outside organization. To fill this information gap, PNNL performed detailed internet searches for each state, which included state agencies, universities, Cooperative Extension Offices, city governments, and related organizations. The state-by-state information on rainwater harvesting regulations was compiled and assembled into an interactive map that is color coded by state regulations. The map provides a visual representation of the general types of rainwater harvesting policies across the country as well as general information on the state programs if applicable. The map allows the user to quickly discern where rainwater harvesting is supported and regulated by the state. This map will be available on the FEMP website by September 2015.

  9. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  10. Cell shape regulates global histone acetylation in human mammaryepithelial cells

    SciTech Connect (OSTI)

    Le Beyec, Johanne; Xu, Ren; Lee, Sun-Young; Nelson, Celeste M.; Rizki, Aylin; Alcaraz, Jordi; Bissell, Mina J.

    2007-02-28

    Extracellular matrix (ECM) regulates cell morphology and gene expression in vivo; these relationships are maintained in three-dimensional (3D) cultures of mammary epithelial cells. In the presence of laminin-rich ECM (lrECM), mammary epithelial cells round up and undergo global histone deacetylation, a process critical for their functional differentiation. However, it remains unclear whether lrECM-dependent cell rounding and global histone deacetylation are indeed part of a common physical-biochemical pathway. Using 3D cultures as well as nonadhesive and micropatterned substrata, here we showed that the cell 'rounding' caused by lrECM was sufficient to induce deacetylation of histones H3 and H4 in the absence of biochemical cues. Microarray and confocal analysis demonstrated that this deacetylation in 3D culture is associated with a global increase in chromatin condensation and a reduction in gene expression. Whereas cells cultured on plastic substrata formed prominent stress fibers, cells grown in 3D lrECM or on micropatterns lacked these structures. Disruption of the actin cytoskeleton with cytochalasin D phenocopied the lrECM-induced cell rounding and histone deacetylation. These results reveal a novel link between ECM-controlled cell shape and chromatin structure, and suggest that this link is mediated by changes in the actin cytoskeleton.

  11. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2003-04-15

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  12. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2008-09-09

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  13. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2005-11-08

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  14. Searchable Electronic Department of Energy Acquisition Regulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon EDEAR July 2 2013 final.pdf More Documents & Publications Microsoft Word - PART 970.doc Department of Energy Acquisition Regulation (DEAR) Final Rule for Subchapters A, B, ...

  15. Regulation of geothermal energy development in Colorado

    SciTech Connect (OSTI)

    Coe, B.A.; Forman, N.A.

    1980-01-01

    The regulatory system is presented in a format to help guide geothermal energy development. State, local, and federal agencies, legislation, and regulations are presented. Information sources are listed. (MHR)

  16. Policy, Regulation, and Codes Technical Assistance

    Broader source: Energy.gov [DOE]

    Policy, regulation, and codes technical assistance includes information and guidance for Indian tribes and Alaska Native villages on energy governance issues. Below is a list of the various options for this type of technical assistance.

  17. Exceptions to DOE Rules and Regulations

    Broader source: Energy.gov [DOE]

    The Department of Energy (DOE) issues test procedures and regulations covering a number of products, but does provide for exceptions to these rules under certain circumstances. Any person, as...

  18. Statutes, Regulations, and Directives for Classification Program |

    Energy Savers [EERE]

    Department of Energy Statutes, Regulations, and Directives for Classification Program Statutes, Regulations, and Directives for Classification Program Classification Atomic Energy Act of 1954 - Establishes Government-wide policies for classifying, safeguarding, and declassifying Restricted Data information. 10 CFR Part 1045, Nuclear Classification and Declassification - Establishes the Government-wide policies and procedures for implementing sections 141 and 142 of the Atomic Energy Act of

  19. Department of Energy Acquisition Regulation (DEAR)

    Broader source: Energy.gov [DOE]

    To ensure that you are using the most recent version of the Department of Energy Acquisition Regulation (DEAR), use one of the electronic versions at one of the following websites: DOE Procurement - http://management.energy.gov/DEAR.htm; Electronic Code of Federal Regulations - http://ecfr.gpoaccess.gov/cgi/t/text/text-idx?c=ecfr&tpl=/ecfrbrowse/Tit... or Hill Air Force Base - http://farsite.hill.af.mil/vfdoea.htm.

  20. International Electricity Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation International Electricity Regulation U.S. trade in electric energy with Canada and Mexico is rising, bringing economic and reliability benefits to the United States and its trading partners. Within the Office of Electricity Delivery & Energy Reliability, the Permitting, Siting and Analysis Division is responsible for authorizing exports of electric energy and issuing Presidential permits for the construction, operation, maintenance and connection of electric transmission

  1. Searchable Electronic Department of Energy Acquisition Regulation |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Searchable Electronic Department of Energy Acquisition Regulation Searchable Electronic Department of Energy Acquisition Regulation Updated July 2, 2013. The EDEAR is current through the Final Rule published May 3, 2013 at 78 FR 25817 If you have any questions concerning this page, please contact Barbara Binney in the Contract and Financial Assistance Policy Division, Office of Acquisition and Project Management. PDF icon EDEAR July 2 2013 final.pdf More Documents &

  2. REGULATIONS ON PHOTOVOLTAIC MODULE DISPOSAL AND RECYCLING.

    SciTech Connect (OSTI)

    FTHENAKIS,V.

    2001-01-29

    Environmental regulations can have a significant impact on product use, disposal, and recycling. This report summarizes the basic aspects of current federal, state and international regulations which apply to end-of-life photovoltaic (PV) modules and PV manufacturing scrap destined for disposal or recycling. It also discusses proposed regulations for electronics that may set the ground of what is to be expected in this area in the near future. In the US, several states have started programs to support the recycling of electronic equipment, and materials destined for recycling often are excepted from solid waste regulations during the collection, transfer, storage and processing stages. California regulations are described separately because they are different from those of most other states. International agreements on the movement of waste between different countries may pose barriers to cross-border shipments. Currently waste moves freely among country members of the Organization of Economic Cooperation and Development (OECD), and between the US and the four countries with which the US has bilateral agreements. However, it is expected, that the US will adopt the rules of the Basel Convention (an agreement which currently applies to 128 countries but not the US) and that the Convection's waste classification system will influence the current OECD waste-handling system. Some countries adopting the Basel Convention consider end-of-life electronics to be hazardous waste, whereas the OECD countries consider them to be non-hazardous. Also, waste management regulations potentially affecting electronics in Germany and Japan are mentioned in this report.

  3. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ø.; Voulgarakis, Nikolaos K.

    2015-03-12

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore » melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  4. Troglitazone induces differentiation in Trypanosoma brucei

    SciTech Connect (OSTI)

    Denninger, Viola; Figarella, Katherine; Schoenfeld, Caroline; Brems, Stefanie; Busold, Christian; Lang, Florian; Hoheisel, Joerg; Duszenko, Michael . E-mail: michael.duszenko@uni-tuebingen.de

    2007-05-15

    Trypanosoma brucei, a protozoan parasite causing sleeping sickness, is transmitted by the tsetse fly and undergoes a complex lifecycle including several defined stages within the insect vector and its mammalian host. In the latter, differentiation from the long slender to the short stumpy form is induced by a yet unknown factor of trypanosomal origin. Here we describe that some thiazolidinediones are also able to induce differentiation. In higher eukaryotes, thiazolidinediones are involved in metabolism and differentiation processes mainly by binding to the intracellular receptor peroxisome proliferator activated receptor {gamma}. Our studies focus on the effects of troglitazone on bloodstream form trypanosomes. Differentiation was monitored using mitochondrial markers (membrane potential, succinate dehydrogenase activity, inhibition of oxygen uptake by KCN, amount of cytochrome transcripts), morphological changes (Transmission EM and light microscopy), and transformation experiments (loss of the Variant Surface Glycoprotein coat and increase of dihydroliponamide dehydrogenase activity). To further investigate the mechanisms responsible for these changes, microarray analyses were performed, showing an upregulation of expression site associated gene 8 (ESAG8), a potential differentiation regulator.

  5. Smart Grid Newsletter ? The Regulators Role in Grid Modernization...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Title: "The Regulator's Role in Grid Modernization" Sponsor: The Modern Grid Strategy is a DOE-funded project conducted by the National Energy Technology Laboratory Leadership from...

  6. Form:Federal Oil and Gas Regulation | Open Energy Information

    Open Energy Info (EERE)

    Federal Oil and Gas Regulation Jump to: navigation, search Federal Oil and Gas Regulation This is the "Federal Oil and Gas Regulation" form. To create a page with this form, enter...

  7. Title 48 CFR 1 Federal Acquisition Regulations System | Open...

    Open Energy Info (EERE)

    8 CFR 1 Federal Acquisition Regulations System Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- Federal RegulationFederal Regulation: Title 48 CFR...

  8. WAC - 173-360 Underground Storage Tank Regulations | Open Energy...

    Open Energy Info (EERE)

    60 Underground Storage Tank Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 173-360 Underground Storage...

  9. Rules and Regulations of the Board of Land Commissioners, Chapter...

    Open Energy Info (EERE)

    Board of Land Commissioners, Chapter 3 - Easements Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Rules and Regulations of...

  10. WAC 173-400 - General Regulations for Air Pollution Sources ...

    Open Energy Info (EERE)

    400 - General Regulations for Air Pollution Sources Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC 173-400 - General...

  11. WAC - 173-400 General Regulations for Air Pollution Sources ...

    Open Energy Info (EERE)

    400 General Regulations for Air Pollution Sources Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 173-400 General...

  12. Rictor regulates FBXW7-dependent c-Myc and cyclin E degradation in colorectal cancer cells

    SciTech Connect (OSTI)

    Guo, Zheng; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Dadao Bei, Guangzhou 510515 ; Zhou, Yuning; Evers, B. Mark; Department of Surgery, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 ; Wang, Qingding; Department of Surgery, The University of Kentucky, 800 Rose Street, Lexington, KY 40536

    2012-02-10

    Highlights: Black-Right-Pointing-Pointer Rictor associates with FBXW7 to form an E3 complex. Black-Right-Pointing-Pointer Knockdown of rictor decreases ubiquitination of c-Myc and cylin E. Black-Right-Pointing-Pointer Knockdown of rictor increases protein levels of c-Myc and cylin E. Black-Right-Pointing-Pointer Overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Black-Right-Pointing-Pointer Rictor regulation of c-Myc and cyclin E requires FBXW7. -- Abstract: Rictor (Rapamycin-insensitive companion of mTOR) forms a complex with mTOR and phosphorylates and activates Akt. Activation of Akt induces expression of c-Myc and cyclin E, which are overexpressed in colorectal cancer and play an important role in colorectal cancer cell proliferation. Here, we show that rictor associates with FBXW7 to form an E3 complex participating in the regulation of c-Myc and cyclin E degradation. The Rictor-FBXW7 complex is biochemically distinct from the previously reported mTORC2 and can be immunoprecipitated independently of mTORC2. Moreover, knocking down of rictor in serum-deprived colorectal cancer cells results in the decreased ubiquitination and increased protein levels of c-Myc and cyclin E while overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Genetic knockout of FBXW7 blunts the effects of rictor, suggesting that rictor regulation of c-Myc and cyclin E requires FBXW7. Our findings identify rictor as an important component of FBXW7 E3 ligase complex participating in the regulation of c-Myc and cyclin E protein ubiquitination and degradation. Importantly, our results suggest that elevated growth factor signaling may contribute to decrease rictor/FBXW7-mediated ubiquitination of c-Myc and cyclin E, thus leading to accumulation of cyclin E and c-Myc in colorectal cancer cells.

  13. Category:Federal Oil and Gas Regulations | Open Energy Information

    Open Energy Info (EERE)

    Regulations Jump to: navigation, search Add a new Federal Oil and Gas Regulation This category currently contains no pages or media. Retrieved from "http:en.openei.orgw...

  14. Idaho IS 61 Public Utility Regulation | Open Energy Information

    Open Energy Info (EERE)

    Regulation (2013). Retrieved from "http:en.openei.orgwindex.php?titleIdahoIS61PublicUtilityRegulation&oldid722586" Feedback Contact needs updating Image needs...

  15. Regulation Indentifier Number Title/Subject/Purpose Rule Type

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1991-AB73 Property Management Regulation: Update and eliminate inconsistencies and redundancies in DOE's personal property management regulations at 41 CFR 109. NOPR Drafting 1991...

  16. 5 CCR 1002-61 Colorado Discharge Permit System Regulations |...

    Open Energy Info (EERE)

    Discharge Permit System RegulationsLegal Abstract Regulations implementing the Colorado Water Quality Control Act applying to all operations discharging to waters of the state...

  17. SERN Policy and Regulation Database | Open Energy Information

    Open Energy Info (EERE)

    SERN Policy and Regulation Database Jump to: navigation, search Tool Summary Name: SERN Policy and Regulation Database AgencyCompany Organization: Renewable Energy and Energy...

  18. California Regulations on Renewble Hydrogen and Low Carbon Technologie...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations on Renewble Hydrogen and Low Carbon Technologies California Regulations on ... Transportation and Stationary Power Integration Workshop: A California Perspective Vision ...

  19. EO 13211: Regulations That Significantly Affect Energy Supply...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EO 13211: Regulations That Significantly Affect Energy Supply, Distribution, or Use EO 13211: Regulations That Significantly Affect Energy Supply, Distribution, or Use I am...

  20. Class Deviation from the Federal Acquisition Regulation (FAR...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation (FAR) 13.5, Test Program for Certain Commercial Items Class Deviation from the Federal Acquisition Regulation (FAR) 13.5, Test Program for Certain Commercial ...

  1. Memorandum: Participation of the EM SSAB, Stakeholders, and Regulators...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Participation of the EM SSAB, Stakeholders, and Regulators in EM Budget Requests Memorandum: Participation of the EM SSAB, Stakeholders, and Regulators in EM Budget Requests From: ...

  2. SUMMARY OF REGULATIONS IMPLEMENTING FEDERAL POWER ACT SECTION...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SUMMARY OF REGULATIONS IMPLEMENTING FEDERAL POWER ACT SECTION 216 SUMMARY OF REGULATIONS IMPLEMENTING FEDERAL POWER ACT SECTION 216 The Energy Policy Act of 2005 added section ...

  3. New Mexico Public Regulation Commission | Open Energy Information

    Open Energy Info (EERE)

    Public Regulation Commission Name: New Mexico Public Regulation Commission Address: 33 Plaza La Prensa Place: Santa Fe, New Mexico Zip: 87507 Website: www.nmprc.state.nm.us...

  4. Natural Gas Regulation - Other Gas-Related Information Sources...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Natural Gas Regulation - Other Gas-Related Information Sources Natural Gas Regulation - Other Gas-Related Information Sources The single largest source of energy information...

  5. Sandia Energy - Self-Regulated Fabrication of Size-Controlled...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Self-Regulated Fabrication of Size-Controlled Quantum Nanostructures Home Highlights - Energy Research Self-Regulated Fabrication of Size-Controlled Quantum Nanostructures Previous...

  6. Building Codes and Regulations for Solar Water Heating Systems...

    Office of Environmental Management (EM)

    Building Codes and Regulations for Solar Water Heating Systems Building Codes and Regulations for Solar Water Heating Systems June 24, 2012 - 1:50pm Addthis Photo Credit:...

  7. Update on Diesel Exhaust Emission Control Technology and Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Control Technology and Regulations Update on Diesel Exhaust Emission Control Technology and Regulations 2004 Diesel Engine Emissions Reduction (DEER) Conference Presentation: ...

  8. Notification of Regulated Waste Activity | Open Energy Information

    Open Energy Info (EERE)

    Notification of Regulated Waste Activity Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- OtherOther: Notification of Regulated Waste...

  9. Guide to Federal Regulation of Sales of Imported Electricity...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States Guide to Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the ...

  10. June 24 Webinar Will Discuss How Environmental Laws and Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Will Discuss How Environmental Laws and Regulations Affect Tribal Energy Projects June 24 Webinar Will Discuss How Environmental Laws and Regulations Affect Tribal Energy...

  11. Renewable Energy Terms of Reference: Laws, Policies and Regulations...

    Open Energy Info (EERE)

    Laws, Policies and Regulations Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Renewable Energy Terms of Reference: Laws, Policies and Regulations AgencyCompany...

  12. Federal Register Notices for DOE NEPA Guidelines and Regulations...

    Office of Environmental Management (EM)

    Notices for DOE NEPA Guidelines and Regulations Federal Register Notices for DOE NEPA Guidelines and Regulations Historical compilation of Federal Register notices for DOE NEPA...

  13. Historical impacts of environmental regulation of silver

    SciTech Connect (OSTI)

    Purcell, T.W.; Peters, J.J.

    1999-01-01

    Exposure to silver, and hence its possible environmental effects, has been regulated since the 1960s. Rules were first developed in the United States to protect against potential negative effects to humans due to ingestion of drinking water containing silver. As regulations for other environmental media were developed, silver was generally included because of its designation as a drinking water chemical of concern. Ambient water quality criteria were developed based on the toxic action of ionic silver. In the early 1990s the US Environmental Protection Agency`s Office of Drinking Water downgraded silver from a primary to a secondary maximum contaminant level because the effects of exposure to silver were judged to be cosmetic. Regulations pertaining to solid waste, surface waters, and occupational exposure have not been changed, but may be changed in the near future as the fate and toxic mechanisms of silver become better understood.

  14. Environmental regulations handbook for enhanced oil recovery

    SciTech Connect (OSTI)

    Madden, M.P. ); Blatchford, R.P.; Spears, R.B. )

    1991-12-01

    This handbook is intended to assist owners and operators of enhanced oil recovery (EOR) operations in acquiring some introductory knowledge of the various state agencies, the US Environmental Protection Agency, and the many environmental laws, rules and regulations which can have jurisdiction over their permitting and compliance activities. It is a compendium of summarizations of environmental rules. It is not intended to give readers specific working details of what is required from them, nor can it be used in that manner. Readers of this handbook are encouraged to contact environmental control offices nearest to locations of interest for current regulations affecting them.

  15. Coping with uncertainties of mercury regulation

    SciTech Connect (OSTI)

    Reich, K.

    2006-09-15

    The thermometer is rising as coal-fired plants cope with the uncertainties of mercury regulation. The paper deals with a diagnosis and a suggested cure. It describes the state of mercury emission rules in the different US states, many of which had laws or rules in place before the Clean Air Mercury Rule (CAMR) was promulgated.

  16. Src regulates the activity of SIRT2

    SciTech Connect (OSTI)

    Choi, You Hee; Kim, Hangun; Lee, Sung Ho; Jin, Yun-Hye; Lee, Kwang Youl

    2014-07-25

    Highlights: Src decreases the protein levels of Sirt2. Src inhibitor and knockdown of Src increase the protein levels of Sirt2. Src interacts with and phosphorylates Sirt2. Src regulate the activity of Sirt2. - Abstract: SIRT2 is a mammalian member of the Sirtuin family of NAD{sup +}-dependent protein deacetylases. The tyrosine kinase Src is involved in a variety of cellular signaling pathways, leading to the induction of DNA synthesis, cell proliferation, and cytoskeletal reorganization. The function of SIRT2 is modulated by post-translational modifications; however, the precise molecular signaling mechanism of SIRT2 through interactions with c-Src has not yet been established. In this study, we investigated the potential regulation of SIRT2 function by c-Src. We found that the protein levels of SIRT2 were decreased by c-Src, and subsequently rescued by the addition of a Src specific inhibitor, SU6656, or by siRNA-mediated knockdown of c-Src. The c-Src interacts with and phosphorylates SIRT2 at Tyr104. c-Src also showed the ability to regulate the deacetylation activity of SIRT2. Investigation on the phosphorylation of SIRT2 suggested that this was the method of c-Src-mediated SIRT2 regulation.

  17. State-federal interactions in nuclear regulation

    SciTech Connect (OSTI)

    Pasternak, A.D.; Budnitz, R.J.

    1987-12-01

    The Atomic Energy Act of 1954 established, and later Congressional amendments have confirmed, that except in areas which have been explicitly granted to the states, the federal government possesses preemptive authority to regulate radiation hazards associated with the development and use of atomic energy. Since the passage of the original Act, numerous decisions by the courts have reaffirmed the legitimacy of federal preemption, and have defined and redefined its scope. In this study, the aim is to explore the underlying issues involved in federal preemption of radiation-hazard regulation, and to recommend actions that the Department of Energy and other agencies and groups should consider undertaking in the near term to protect the preemption principle. Appropriate roles of the states are discussed, as well as recent state-level activities and their rationale, and several current arenas in which state-federal conflicts about regulation of hazards are being played out. The emphasis here is on four particular arenas that are now important arenas of conflict, but the issues discussed are far broader in scope. These four arenas are: state-level moratorium activity; emergency planning for reactors; conflicts arising from state financial regulation; and inroads in federal preemption through litigation under state law.

  18. Method for regulation of plant lignin composition

    DOE Patents [OSTI]

    Chapple, Clint (West Lafayette, IN)

    1999-01-01

    A method is disclosed for the regulation of lignin composition in plant tissue. Plants are transformed with a gene encoding an active F5H gene. The expression of the F5H gene results in increased levels of syringyl monomer providing a lignin composition more easily degraded with chemicals and enzymes.

  19. Voltage regulation in linear induction accelerators

    DOE Patents [OSTI]

    Parsons, William M.

    1992-01-01

    Improvement in voltage regulation in a Linear Induction Accelerator wherein a varistor, such as a metal oxide varistor, is placed in parallel with the beam accelerating cavity and the magnetic core. The non-linear properties of the varistor result in a more stable voltage across the beam accelerating cavity than with a conventional compensating resistance.

  20. Voltage regulation in linear induction accelerators

    DOE Patents [OSTI]

    Parsons, W.M.

    1992-12-29

    Improvement in voltage regulation in a linear induction accelerator wherein a varistor, such as a metal oxide varistor, is placed in parallel with the beam accelerating cavity and the magnetic core is disclosed. The non-linear properties of the varistor result in a more stable voltage across the beam accelerating cavity than with a conventional compensating resistance. 4 figs.

  1. 49 CFR Parts 171-177: Hazardous Materials Regulations (DOT)

    Broader source: Energy.gov [DOE]

    The U.S. Department of Transportation Pipeline and Hazardous Materials Safety Administration regulates the transport of hazardous materials through Title 49 of the Code of Federal Regulations (49 CFR), Subchapter C, "Hazardous Materials Regulations." Parts 171-177 provide general information on hazardous materials and regulation for their packaging and their shipment by rail, air, vessel, and public highway.

  2. Federal Regulations | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Federal Regulations Human Subjects Protection Program (HSPP) HSPP Home About Institutional Review Boards (IRBs) Education and Resources Regulations and Requirements Ethical Principles Federal Regulations DOE Special Requirements Health Insurance Portability and Accountability Act (HIPAA) Glossary Abbreviations Contact BER Home Contact Information Human Subjects Protection Program U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 Regulations and

  3. Oxidative stress-induced autophagy: Role in pulmonary toxicity

    SciTech Connect (OSTI)

    Malaviya, Rama; Laskin, Jeffrey D.; Laskin, Debra L.

    2014-03-01

    Autophagy is an evolutionarily conserved catabolic process important in regulating the turnover of essential proteins and in elimination of damaged organelles and protein aggregates. Autophagy is observed in the lung in response to oxidative stress generated as a consequence of exposure to environmental toxicants. Whether autophagy plays role in promoting cell survival or cytotoxicity is unclear. In this article recent findings on oxidative stress-induced autophagy in the lung are reviewed; potential mechanisms initiating autophagy are also discussed. A better understanding of autophagy and its role in pulmonary toxicity may lead to the development of new strategies to treat lung injury associated with oxidative stress. - Highlights: Exposure to pulmonary toxicants is associated with oxidative stress. Oxidative stress is known to induce autophagy. Autophagy is upregulated in the lung following exposure to pulmonary toxicants. Autophagy may be protective or pathogenic.

  4. Collegiate Wind Competition 2014 Rules and Regulations | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy 2014 Rules and Regulations Collegiate Wind Competition 2014 Rules and Regulations This document outlines the rules and regulations for the inaugural Collegiate Wind Competition in 2014. PDF icon U.S. Department of Energy Collegiate Wind Competition 2014 Rules and Regulations More Documents & Publications Collegiate Wind Competition Rules and Regulations Team roster: Andrew Dallas, Aerospace Engineering; Mario Mondal, Aerospace Engineering; Atif Salahudeen, Aerospace Engineering;

  5. Notice of 2009 Guidance on Energy Efficiency Enforcement Regulations |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 2009 Guidance on Energy Efficiency Enforcement Regulations Notice of 2009 Guidance on Energy Efficiency Enforcement Regulations Notice - DOE Guidance on Energy Efficiency Enforcement Regulations, Federal Register Vol. 74, No. 197 October 14, 2009. This notice sets forth the Department of Energy's (DOE's) interpretation of its energy efficiency enforcement regulations. These regulations provide for manufacturer submission of compliance statements and certification reports

  6. Apigenin suppresses migration and invasion of transformed cells through down-regulation of C-X-C chemokine receptor 4 expression

    SciTech Connect (OSTI)

    Wang, Lei; Kuang, Lisha; Hitron, John Andrew; Son, Young-Ok; Wang, Xin; Budhraja, Amit; Lee, Jeong-Chae; Pratheeshkumar, Poyil; Chen, Gang; Zhang, Zhuo; Luo, Jia; Shi, Xianglin

    2013-10-01

    Environmental exposure to arsenic is known to cause various cancers. There are some potential relationships between cell malignant transformation and C-X-C chemokine receptor type 4 (CXCR4) expressions. Metastasis, one of the major characteristics of malignantly transformed cells, contributes to the high mortality of cells. CXCR4 and its natural chemokine ligand C-X-C motif ligand 12 (CXCL12) play a critical role in metastasis. Therefore, identification of nutritional factors which are able to inhibit CXCR4 is important for protection from environmental arsenic-induced carcinogenesis and for abolishing metastasis of malignantly transformed cells. The present study demonstrates that apigenin (4′,5,7-trihydroxyflavone), a natural dietary flavonoid, suppressed CXCR4 expression in arsenic-transformed Beas-2B cells (B-AsT) and several other types of transformed/cancer cells in a dose- and time-dependent manner. Neither proteasome nor lysosome inhibitor had any effect in reducing the apigenin-induced down-regulation of CXCR4, indicating that apigenin-induced down-regulation of CXCR4 is not due to proteolytic degradation. The down-regulation of CXCR4 is mainly due to the inhibition of nuclear factor κB (NF-κB) transcriptional activity. Apigenin also abolished migration and invasion of transformed cells induced by CXCL12. In a xenograft mouse model, apigenin down-regulated CXCR4 expression and suppressed tumor growth. Taken together, our results show that apigenin is a novel inhibitor of CXCR4 expression. This dietary flavonoid has the potential to suppress migration and invasion of transformed cells and prevent environmental arsenic-induced carcinogenesis. - Highlights: • Apigenin has a potential in preventing environmental arsenic induced carcinogenesis. • Apigenin suppresses CXCR4 in malignant transformed cells in vitro and in vivo. • The down-regulation of CXCR4 is mainly due to inhibition of NF-κB activity.

  7. Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation

    SciTech Connect (OSTI)

    Reed, J.C.; Tsujimoto, Y.; Alpers, J.D.; Croce, C.M.; Nowell, P.C.

    1987-06-05

    The bcl-2 and c-myc proto-oncogenes are brought into juxtaposition with the immuno-globulin heavy chain locus in particular B-cell lymphomas, resulting in high levels of constitutive accumulation of their messenger RNAs. Precisely how the products of the bcl-2 and c-myc genes contribute to tumorigenesis is unknown, but observations that c-myc expression is rapidly induced in nonneoplastic lymphocytes upon stimulation of proliferation raise the possibility that this proto-oncogene is involved in the control of normal cellular growth. In addition to c-myc, the bcl-2 proto-oncogene also was expressed in normal human B and T lymphocytes after stimulation with appropriate mitogens. Comparison of the regulation of the expression of these proto-oncogenes demonstrated marked differences and provided evidence that, in contrast to c-myc, levels of bcl-2 messenger RNA are regulated primarily though transcriptional mechanisms. 10 references, 3 figures.

  8. Regulation of flexible arms under gravity

    SciTech Connect (OSTI)

    De Luca, A.; Siciliano, B.

    1993-08-01

    A simple controller is presented for the regulation problem of robot arms with flexible links under gravity. It consists of a joint PD feedback plus a constant feedforward. Global asymptotic stability of the reference equilibrium state is shown under a structural assumption about link elasticity and a mild condition on the proportional gain. The result holds also in the absence of internal damping of the flexible arm. A numerical case study is presented.

  9. Laser Induced Spectroscopy - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laser Induced Spectroscopy Idaho National Laboratory Contact INL About This Technology Technology Marketing Summary INL's Laser Induced Spectroscopy technology detects and measures ...

  10. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Disorder-Induced Microscopic Magnetic Memory Print Wednesday, 26 October 2005 00:00 The magnetic-recording industry deliberately...

  11. Mechanisms of the hepatoprotective effects of tamoxifen against drug-induced and chemical-induced acute liver injuries

    SciTech Connect (OSTI)

    Yoshikawa, Yukitaka; Miyashita, Taishi; Higuchi, Satonori; Tsuneyama, Koichi; Endo, Shinya; Tsukui, Tohru; Toyoda, Yasuyuki; Fukami, Tatsuki; Nakajima, Miki; Yokoi, Tsuyoshi

    2012-10-01

    Although estrogen receptor (ER)? agonists, such as estradiol and ethinylestradiol (EE2), cause cholestasis in mice, they also reduce the degree of liver injury caused by hepatotoxicants as well as ischemiareperfusion. The functional mechanisms of ER? have yet to be elucidated in drug-induced or chemical-induced liver injury. The present study investigated the effects of an ER? agonist, selective ER modulators (SERMs) and an ER antagonist on drug-induced and chemical-induced liver injuries caused by acetaminophen, bromobenzene, diclofenac, and thioacetamide (TA). We observed hepatoprotective effects of EE2, tamoxifen (TAM) and raloxifene pretreatment in female mice that were exposed to a variety of hepatotoxic compounds. In contrast, the ER antagonist did not show any hepatoprotective effects. DNA microarray analyses suggested that monocyte to macrophage differentiation-associated 2 (Mmd2) protein, which has an unknown function, is commonly increased by TAM and RAL pretreatment, but not by pretreatment with the ER antagonist. In ER?-knockout mice, the hepatoprotective effects of TAM and the increased expression of Mmd2 mRNA were not observed in TA-induced liver injury. To investigate the function of Mmd2, the expression level of Mmd2 mRNA was significantly knocked down to approximately 30% in mice by injection of siRNA for Mmd2 (siMmd2). Mmd2 knockdown resulted in a reduction of the protective effects of TAM on TA-induced liver injury in mice. This is the first report of the involvement of ER? in drug-induced or chemical-induced liver injury. Upregulation of Mmd2 protein in the liver was suggested as the mechanism of the hepatoprotective effects of EE2 and SERMs. -- Highlights: ? Liver injury induced by drugs or chemicals was investigated in mice. ? Liver injury was suppressed by pretreatment with tamoxifen in female mice. ? Mmd2, whose function was unknown, could be a candidate gene for liver protection. ? Tamoxifen up-regulated Mmd2 mRNA expression via ER?.

  12. Hedgehog pathway regulators influence cervical cancer cell proliferation, survival and migration

    SciTech Connect (OSTI)

    Samarzija, Ivana; Beard, Peter

    2012-08-17

    Highlights: Black-Right-Pointing-Pointer Unknown cellular mutations complement papillomavirus-induced carcinogenesis. Black-Right-Pointing-Pointer Hedgehog pathway components are expressed by cervical cancer cells. Black-Right-Pointing-Pointer Hedgehog pathway activators and inhibitors regulate cervical cancer cell biology. Black-Right-Pointing-Pointer Cell immortalization by papillomavirus and activation of Hedgehog are independent. -- Abstract: Human papillomavirus (HPV) infection is considered to be a primary hit that causes cervical cancer. However, infection with this agent, although needed, is not sufficient for a cancer to develop. Additional cellular changes are required to complement the action of HPV, but the precise nature of these changes is not clear. Here, we studied the function of the Hedgehog (Hh) signaling pathway in cervical cancer. The Hh pathway can have a role in a number of cancers, including those of liver, lung and digestive tract. We found that components of the Hh pathway are expressed in several cervical cancer cell lines, indicating that there could exists an autocrine Hh signaling loop in these cells. Inhibition of Hh signaling reduces proliferation and survival of the cervical cancer cells and induces their apoptosis as seen by the up-regulation of the pro-apoptotic protein cleaved caspase 3. Our results indicate that Hh signaling is not induced directly by HPV-encoded proteins but rather that Hh-activating mutations are selected in cells initially immortalized by HPV. Sonic Hedgehog (Shh) ligand induces proliferation and promotes migration of the cervical cancer cells studied. Together, these results indicate pro-survival and protective roles of an activated Hh signaling pathway in cervical cancer-derived cells, and suggest that inhibition of this pathway may be a therapeutic option in fighting cervical cancer.

  13. Fibroblast growth factor-2 up-regulates the expression of nestin through the RasRafERKSp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: Nestin expression in C6 glioma cells is induced by FGF-2. Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. The mRNA of FGFR1 and 3 are detected in C6 glioma cells. RasRafERKSp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFRMAPKERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  14. Evolving Role of the Power Sector Regulator: A Clean Energy Regulators Initiative Report

    SciTech Connect (OSTI)

    Zinaman, O.; Miller, M.; Bazilian, M.

    2014-04-01

    This paper seeks to briefly characterize the evolving role of power sector regulation. Given current global dynamics, regulation of the power sector is undergoing dramatic changes. This transformation is being driven by various factors including technological advances and cost reductions in renewable energy, energy efficiency, and demand management; increasing air pollution and climate change concerns; and persistent pressure for ensuring sustainable economic development and increased access to energy services by the poor. These issues add to the already complex task of power sector regulation, of which the fundamental remit remains to objectively and transparently ensure least-cost service delivery at high quality. While no single regulatory task is trivial to undertake, it is the prioritization and harmonization of a multitude of objectives that exemplifies the essential challenge of power sector regulation. Evolving regulatory roles can be understood through the concept of existing objectives and an additional layer of emerging objectives. Following this categorization, we describe seven existing objectives of power sector regulators and nine emerging objectives, highlighting key challenges and outlining interdependencies. This essay serves as a preliminary installment in the Clean Energy Regulatory Initiative (CERI) series, and aims to lay the groundwork for subsequent reports and case studies that will explore these topics in more depth.

  15. Environmental Regulation Impacts on Eastern Interconnection Performance

    SciTech Connect (OSTI)

    Markham, Penn N; Liu, Yilu; Young II, Marcus Aaron

    2013-07-01

    In the United States, recent environmental regulations will likely result in the removal of nearly 30 GW of oil and coal-fired generation from the power grid, mostly in the Eastern Interconnection (EI). The effects of this transition on voltage stability and transmission line flows have previously not been studied from a system-wide perspective. This report discusses the results of power flow studies designed to simulate the evolution of the EI over the next few years as traditional generation sources are replaced with environmentally friendlier ones such as natural gas and wind.

  16. Self-regulating flow control device

    DOE Patents [OSTI]

    Humphreys, Duane A.

    1984-01-01

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  17. Ligand-specific regulation of the extracellular surface of a...

    Office of Scientific and Technical Information (OSTI)

    Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor Citation Details In-Document Search Title: Ligand-specific regulation of the extracellular ...

  18. 5 CCR 1002-64 Biosolids Regulation | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- OtherOther: 5 CCR 1002-64 Biosolids RegulationLegal Abstract Regulations implementing the Colorado...

  19. RCRA Notification of Regulated Waste Activity (EPA Form 8700...

    Open Energy Info (EERE)

    Notification of Regulated Waste Activity (EPA Form 8700-12) Jump to: navigation, search OpenEI Reference LibraryAdd to library Form: RCRA Notification of Regulated Waste Activity...

  20. Colorado Parks and Wildlife Rules and Regulations | Open Energy...

    Open Energy Info (EERE)

    Rules and Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Colorado Parks and Wildlife Rules and Regulations Abstract This web page lists...

  1. Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY The regulations in this part are issued for the protection and security of facilities, installations and real property subject to the jurisdiction or administration, or in the custody of, the Department of Energy. PDF icon Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY More Documents & Publications Code of Federal Regulations

  2. Contacts for the Assistant General Counsel for Legislation, Regulation, and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Efficiency | Department of Energy Legislation, Regulation, and Energy Efficiency Contacts for the Assistant General Counsel for Legislation, Regulation, and Energy Efficiency Office of the Assistant General Counsel for Legislation, Regulation & Energy Efficiency (GC-33) Daniel Cohen, Assistant General Counsel for Legislation, Regulation, & Energy Efficiency 202-586-9523 daniel.cohen@hq.doe.gov Catherine Goshe, Attorney-Adviser 202-586-7053 Preeti Chaudhari, Attorney-Adviser

  3. Method to determine transcriptional regulation pathways in organisms

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Method to determine transcriptional regulation pathways in organisms Citation Details In-Document Search Title: Method to determine transcriptional regulation pathways in organisms The invention relates to computer-implemented methods and systems for identifying regulatory relationships between expressed regulating polypeptides and targets of the regulatory activities of such regulating polypeptides. More specifically, the invention provides a new method for

  4. Using regulations.gov to find dockets and documents

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    regulations.gov to find dockets and documents Regulations.gov is a web-based repository of Federal regulatory documents. Publicly accessible documents associated with DOE Appliance and Equipment Standards rulemakings and related Federal Register notices are now accessible on regulations.gov On regulations.gov, you can: View Proposed and Final Rules and other regulatory documents or notices Read public comments, as well as public meeting presentations and transcripts These documents are part of a

  5. North America: Regulation of International Electricity Trade | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy America: Regulation of International Electricity Trade North America: Regulation of International Electricity Trade North America: Regulation of International Electricity Trade prepared by North American Energy Working Group on December 2002. PDF icon North America: Regulation of International Electricity Trade More Documents & Publications Regulatory Side-by-Side Governing Permitting of Cross-Border Electricity Transmission Facilities Between the United States and Canada Guide

  6. DOE Review of Regulations - Center for Regulatory Effectiveness Comments |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Review of Regulations - Center for Regulatory Effectiveness Comments DOE Review of Regulations - Center for Regulatory Effectiveness Comments The Center for Regulatory Effectiveness (CRE) is pleased to submit these comments to the Department of Energy (DOE) on how DOE can best review its existing regulations and to identify whether any of its existing regulations should be modified, streamlined, expanded or repealed. PDF icon

  7. EA-1631: Beacon Power Corporation Frequency Regulation Facility in

    Energy Savers [EERE]

    Stephentown, NY | Department of Energy 1: Beacon Power Corporation Frequency Regulation Facility in Stephentown, NY EA-1631: Beacon Power Corporation Frequency Regulation Facility in Stephentown, NY February 2, 2009 EA-1631: Final Environmental Assessment Loan Guarantee for Beacon Power Corporation Frequency Regulation Facility in Stephentown, New York February 27, 2009 EA-1631: Finding of No Significant Impact Loan Guarantee for Beacon Power Corporation Frequency Regulation Facility in

  8. QB1 - Stochastic Gene Regulation Munsky, Brian [Los Alamos National

    Office of Scientific and Technical Information (OSTI)

    FACILITIES; FLUCTUATIONS; GENE REGULATION; KINETICS; QUANTIZATION; SIMULATION; SWITCHES Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present...

  9. NARUC Releases Cybersecurity Primer for Utility Regulators (June 2012) |

    Energy Savers [EERE]

    Department of Energy NARUC Releases Cybersecurity Primer for Utility Regulators (June 2012) NARUC Releases Cybersecurity Primer for Utility Regulators (June 2012) June 14, 2012 - 4:50pm Addthis The National Association of Regulatory Utility Commissioners (NARUC) has released "Cybersecurity for State Regulators," a primer that explains conceptual cybersecurity basics and points to additional resources that can help regulators develop internal cybersecurity expertise, ask questions

  10. Guidance Regarding NEPA Regulations (CEQ, 1983) | Department of Energy

    Energy Savers [EERE]

    NEPA Regulations (CEQ, 1983) Guidance Regarding NEPA Regulations (CEQ, 1983) This document provides Council on Environmental Quality guidance on several topics: scoping, categorical exclusions, adoption procedures, contracting provisions, selection of alternatives in licensing and permitting situations, and tiering. PDF icon Guidance Regarding NEPA Regulations More Documents & Publications Final Guidance for Effective Use of Programmatic NEPA Review Forty Most Asked Questions Concerning

  11. Regulation of Viable and Optimal Cohorts

    SciTech Connect (OSTI)

    Aubin, Jean-Pierre

    2015-10-15

    This study deals with the evolution of (scalar) attributes (resources or income in evolutionary demography or economics, position in traffic management, etc.) of a population of “mobiles” (economic agents, vehicles, etc.). The set of mobiles sharing the same attributes is regarded as an instantaneous cohort described by the number of its elements. The union of instantaneous cohorts during a mobile window between two attributes is a cohort. Given a measure defining the number of instantaneous cohorts, the accumulation of the mobile attributes on a evolving mobile window is the measure of the cohort on this temporal mobile window. Imposing accumulation constraints and departure conditions, this study is devoted to the regulation of the evolutions of the attributes which are1.viable in the sense that the accumulations constraints are satisfied at each instant;2.and, among them, optimal, in the sense that both the duration of the temporal mobile window is maximum and that the accumulation on this temporal mobile window is the largest viable one. This value is the “accumulation valuation” function. Viable and optimal evolutions under accumulation constraints are regulated by an “implicit Volterra integro-differential inclusion” built from the accumulation valuation function, solution to an Hamilton–Jacobi–Bellman partial differential equation under constraints which is constructed for this purpose.

  12. Dancing with the regulations - Part Deux

    SciTech Connect (OSTI)

    Nitschke, R.L.

    1995-12-31

    The disposal of low-level radioactive waste (LLW) in the United States has long been subjected to two very similar regulations depending upon the location. Disposal sites located on Department of Energy (DOE) Reservations are subject to DOE Order 5820.2A {open_quotes}Radioactive Waste Management,{close_quotes} while disposal sites located elsewhere are subject to the Nuclear Regulatory Commission regulation 10 CFR 61 {open_quotes}Licensing Requirements for Land Disposal of Radioactive Waste.{close_quotes} While life was not necessarily good, there was only one sheet of music to dance to. Recently a new player, named CERCLA (Comprehensive Environmental Response, Compensation, and Liability Act), has ridden into those DOE towns, and for those whose disposal facilities lie within or adjacent to Superfund sites, she has brought along a different drum to dance to. This paper discusses the differences and similarities between the different dance partners and their associated musical scores (i.e., the performance assessment (PA) required by the DOE order and the baseline risk assessment (BRA) required by CERCLA). The paper then provides a brief discussion on the latest dancer to cut in: the Defense Nuclear Facilities Safety Board (DNFSB). This discussion should help to alleviate the confusion while dancing on the LLW disposal regulatory ballroom floor.

  13. cAMP-response-element-binding protein positively regulates breast cancer metastasis and subsequent bone destruction

    SciTech Connect (OSTI)

    Son, Jieun; Lee, Jong-Ho; Kim, Ha-Neui; Ha, Hyunil Lee, Zang Hee

    2010-07-23

    Research highlights: {yields} CREB is highly expressed in advanced breast cancer cells. {yields} Tumor-related factors such as TGF-{beta} further elevate CREB expression. {yields} CREB upregulation stimulates metastatic potential of breast cancer cells. {yields} CREB signaling is required for breast cancer-induced bone destruction. -- Abstract: cAMP-response-element-binding protein (CREB) signaling has been reported to be associated with cancer development and poor clinical outcome in various types of cancer. However, it remains to be elucidated whether CREB is involved in breast cancer development and osteotropism. Here, we found that metastatic MDA-MB-231 breast cancer cells exhibited higher CREB expression than did non-metastatic MCF-7 cells and that CREB expression was further increased by several soluble factors linked to cancer progression, such as IL-1, IGF-1, and TGF-{beta}. Using wild-type CREB and a dominant-negative form (K-CREB), we found that CREB signaling positively regulated the proliferation, migration, and invasion of MDA-MB-231 cells. In addition, K-CREB prevented MDA-MB-231 cell-induced osteolytic lesions in a mouse model of cancer metastasis. Furthermore, CREB signaling in cancer cells regulated the gene expression of PTHrP, MMPs, and OPG, which are closely involved in cancer metastasis and bone destruction. These results indicate that breast cancer cells acquire CREB overexpression during their development and that this CREB upregulation plays an important role in multiple steps of breast cancer bone metastasis.

  14. Liver X Receptor (LXR) activation negatively regulates visfatin expression in macrophages

    SciTech Connect (OSTI)

    Mayi, Therese Hervee; Rigamonti, Elena; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Pattou, Francois; Department of Endocrine Surgery, University Hospital, Lille; U859 Biotherapies for Diabetes, INSERM, Lille ; Staels, Bart; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Chinetti-Gbaguidi, Giulia; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille

    2011-01-07

    Research highlights: {yields} Synthetic LXR ligands decreased visfatin expression in human macrophages. {yields} LXR activation leads to a modest and transient decrease of NAD{sup +} concentration. {yields} LXR activation decreased PPAR{gamma}-induced visfatin in human macrophages. -- Abstract: Adipose tissue macrophages (ATM) are the major source of visfatin, a visceral fat adipokine upregulated during obesity. Also known to play a role in B cell differentiation (pre-B cell colony-enhancing factor (PBEF)) and NAD biosynthesis (nicotinamide phosphoribosyl transferase (NAMPT)), visfatin has been suggested to play a role in inflammation. Liver X Receptor (LXR) and Peroxisome Proliferator-Activated Receptor (PPAR){gamma} are nuclear receptors expressed in macrophages controlling the inflammatory response. Recently, we reported visfatin as a PPAR{gamma} target gene in human macrophages. In this study, we examined whether LXR regulates macrophage visfatin expression. Synthetic LXR ligands decreased visfatin gene expression in a LXR-dependent manner in human and murine macrophages. The decrease of visfatin mRNA was paralleled by a decrease of protein secretion. Consequently, a modest and transient decrease of NAD{sup +} concentration was observed. Interestingly, LXR activation decreased the PPAR{gamma}-induced visfatin gene and protein secretion in human macrophages. Our results identify visfatin as a gene oppositely regulated by the LXR and PPAR{gamma} pathways in human macrophages.

  15. Irreversible inhibition of RANK expression as a possible mechanism for IL-3 inhibition of RANKL-induced osteoclastogenesis

    SciTech Connect (OSTI)

    Khapli, Shruti M.; Tomar, Geetanjali B.; Barhanpurkar, Amruta P.; Gupta, Navita; Yogesha, S.D.; Pote, Satish T.; Wani, Mohan R.

    2010-09-03

    Research highlights: {yields} IL-3 inhibits receptor activator of NF-{kappa}B ligand (RANKL)-induced osteoclastogenesis. {yields} IL-3 inhibits RANKL-induced JNK activation. {yields} IL-3 down-regulates expression of c-Fos and NFATc1 transcription factors. {yields} IL-3 down-regulates RANK expression posttranscriptionally and irreversibly. {yields} IL-3 inhibits in vivo RANK expression. -- Abstract: IL-3, a cytokine secreted by activated T lymphocytes, stimulates the proliferation, differentiation and survival of pluripotent hematopoietic stem cells. In this study, we investigated the mechanism of inhibitory action of IL-3 on osteoclast differentiation. We show here that IL-3 significantly inhibits receptor activator of NF-{kappa}B (RANK) ligand (RANKL)-induced activation of c-Jun N-terminal kinase (JNK). IL-3 down-regulates expression of c-Fos and nuclear factor of activated T cells (NFATc1) transcription factors. In addition, IL-3 down-regulates RANK expression posttranscriptionally in both purified osteoclast precursors and whole bone marrow cells. Furthermore, the inhibitory effect of IL-3 on RANK expression was irreversible. Interestingly, IL-3 inhibits in vivo RANK expression in mice. Thus, we provide the first evidence that IL-3 irreversibly inhibits RANK expression that results in inhibition of important signaling molecules induced by RANKL.

  16. Rac1 and Cdc42 GTPases regulate shear stress-driven ?-catenin signaling in osteoblasts

    SciTech Connect (OSTI)

    Wan, Qiaoqiao; Cho, Eunhye; Yokota, Hiroki; Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN 46202 ; Na, Sungsoo

    2013-04-19

    Highlights: Shear stress increased TCF/LEF activity and stimulated ?-catenin nuclear localization. Rac1, Cdc42, and RhoA displayed distinct dynamic activity patterns under flow. Rac1 and Cdc42, but not RhoA, regulate shear stress-driven TCF/LEF activation. Cytoskeleton did not significantly affect shear stress-induced TCF/LEF activation. -- Abstract: Beta-catenin-dependent TCF/LEF (T-cell factor/lymphocyte enhancing factor) is known to be mechanosensitive and an important regulator for promoting bone formation. However, the functional connection between TCF/LEF activity and Rho family GTPases is not well understood in osteoblasts. Herein we investigated the molecular mechanisms underlying oscillatory shear stress-induced TCF/LEF activity in MC3T3-E1 osteoblast cells using live cell imaging. We employed fluorescence resonance energy transfer (FRET)-based and green fluorescent protein (GFP)-based biosensors, which allowed us to monitor signal transduction in living cells in real time. Oscillatory (1 Hz) shear stress (10 dynes/cm{sup 2}) increased TCF/LEF activity and stimulated translocation of ?-catenin to the nucleus with the distinct activity patterns of Rac1 and Cdc42. The shear stress-induced TCF/LEF activity was blocked by the inhibition of Rac1 and Cdc42 with their dominant negative mutants or selective drugs, but not by a dominant negative mutant of RhoA. In contrast, constitutively active Rac1 and Cdc42 mutants caused a significant enhancement of TCF/LEF activity. Moreover, activation of Rac1 and Cdc42 increased the basal level of TCF/LEF activity, while their inhibition decreased the basal level. Interestingly, disruption of cytoskeletal structures or inhibition of myosin activity did not significantly affect shear stress-induced TCF/LEF activity. Although Rac1 is reported to be involved in ?-catenin in cancer cells, the involvement of Cdc42 in ?-catenin signaling in osteoblasts has not been identified. Our findings in this study demonstrate that both Rac1 and Cdc42 GTPases are critical regulators in shear stress-driven ?-catenin signaling in osteoblasts.

  17. Is anyone regulating naturally occurring radioactive material? A state survey

    SciTech Connect (OSTI)

    Gross, E.M.; Barisas, S.G.

    1993-08-01

    As far as we know, naturally occurring radioactive material (NORM) has surrounded humankind since the beginning of time. However, recent data demonstrating that certain activities concentrate NORM have increased concern regarding its proper handling and disposal and precipitated the development of new NORM-related regulations. The regulation of NORM affects the management of government facilities as well as a broad range of industrial processes. Recognizing that NORM regulation at the federal level is extremely limited, Argonne National Laboratory (ANL) conducted a 50-state survey to determine the extent to which states have assumed the responsibility for regulating NORM as well as the NORM standards that are currently being applied at the state level. Though the survey indicates that NORM regulation comprises a broad spectrum of controls from full licensing requirements to virtually no regulation at afl, a trend is emerging toward recognition of the need for increased regulation of potential NORM hazards, particularly in the absence of federal standards.

  18. When regulations go too far the taking of private property by environmental regulations

    SciTech Connect (OSTI)

    Plott, D.M.

    1995-12-01

    Seventy years ago Justice Oliver Wendell Homes wrote in an Opinion of the United States Supreme Court that{open_quotes}...while property may be regulated to a certain extent, if regulation goes too far it will be recognized as a taking.{close_quotes} Pennsylvania Coal Co. v. Mahon, 260 U.S. 393 (1992) The Fifth Amendment of the United States Constitution provides that private property shall not be {open_quotes}taken for public use, without just compensation{close_quotes}. After lying dormant for half a century, the {open_quotes}takings{close_quotes} clause of the United States constitution is being invoked to challenge environmental regulations throughout the country. In essence, property owners are saying that if the government wants to regulate to protect wetlands, wildlife habitat and other natural resources it must pay just compensation to the property owners for the restrictions imposed upon the use of their land. During the past ten years, the U.S. Supreme Court has decided several takings cases. While the judicial branch of government has become increasingly active in takings cases, the U.S. Congress and State legislatures have also been extremely active in efforts to protect private property rights. Typically, these legislative requires a takings impact analysis by the government to determine if its actions are likely to result in the compensable taking of private property or require compensation if property values are reduced by a certain percentage. This paper reviews the judicial standards that have been established for reviewing taking claims and sets forth a framework for analyzing when a regulation goes {open_quotes}too far{close_quotes} so as to require payment of compensation.

  19. System and method for regulating resonant inverters

    DOE Patents [OSTI]

    Stevanovic, Ljubisa Dragoljub (Clifton Park, NY); Zane, Regan Andrew (Superior, CO)

    2007-08-28

    A technique is provided for direct digital phase control of resonant inverters based on sensing of one or more parameters of the resonant inverter. The resonant inverter control system includes a switching circuit for applying power signals to the resonant inverter and a sensor for sensing one or more parameters of the resonant inverter. The one or more parameters are representative of a phase angle. The resonant inverter control system also includes a comparator for comparing the one or more parameters to a reference value and a digital controller for determining timing of the one or more parameters and for regulating operation of the switching circuit based upon the timing of the one or more parameters.

  20. Body temperature regulation during euthermia and hyperthermia

    SciTech Connect (OSTI)

    Shimada, S.G.; Stitt, J.T.

    1981-10-01

    In this paper we have presented a few fundamental principles which are essential for an understanding of how microwaves might affect body temperature regulation. Application of the heat balance equation requires an accounting of all heat losses and heat gains. Thus, experimental measurements of these two processes are usually required to explain any thermoregulatory response. Knowledge of the individual elements of the thermoregulatory system and their integrative operation is imperative. Without such information one cannot hope to analyze or predict the consequences of microwave irradiation, or to separate simple heating effects from possible nonthermal effects. Finally, we have tried to emphasize that hyperthermia can be produced in different ways. Thus, there may be several forms of microwave hyperthermia, differing in their causality. Deposition of microwave energy in deep tissues might produce a response similar to exercise hyperthermia.

  1. Economics and regulation of petroleum futures markets

    SciTech Connect (OSTI)

    Not Available

    1980-08-01

    Because the futures market in petroleum products is a relatively recent phenomenon, the implications of public policies formulated for that market have not yet been fully explored. To provide the Office of Competition of the Department of Energy (DOE) with sufficient information to assess policy alternatives, Resource Planning Associates, Inc. (RPA) was asked to analyze the development of the futures market in No. 2 oil, assess the potential for futures markets in other petroleum products, and identify policy alternatives available to DOE. To perform this analysis, the criteria for a viable futures market was established first. Then, the experience to date with the 18-month-old futures market in No. 2 oil was examined, and the potential for viable futures markets in No. 6 oil, gasoline, jet fuel, and crude oil was assessed. Finally, how existing DOE regulations and prospective actions might affect petroleum futures market development was investigated.

  2. PES1 regulates sensitivity of colorectal cancer cells to anticancer drugs

    SciTech Connect (OSTI)

    Xie, Wei; Qu, Like; Meng, Lin; Liu, Caiyun; Wu, Jian; Shou, Chengchao

    2013-02-15

    Highlights: ? PES1 was overexpressed in diverse cancer cell lines. ? PES1-ablation enhances DNA damage response by decreasing DNA repair. ? PES1-ablation increases the sensitivity of HCT116 cells to chemotherapeutic agents. ? PES1-ablation is associated with diminished nuclear entry of RAD51. -- Abstract: PES1 (also known as Pescadillo), a nucleolar protein, was involved in biogenesis of ribosomal RNA. Up-regulation of PES1 has been documented in some human cancers, indicating that PES1 may play some crucial roles in tumorigenesis. In our previous study, it was found that silencing of PES1 resulted in decreased proliferation of colorectal cancer cells. We also noticed that depletion of PES1 altered expression profiles of diverse genes. In the present study, we validated the expression changes of a subset of genotoxic stress-related genes in PES1-silenced HCT116 cells by quantitative RT-PCR. The steady and etoposide-induced phosphorylated H2AX (?-H2AX) were higher in PES1-silenced cells than in control cells. Besides, etoposide-induced ?-H2AX persisted longer in PES1-silenced cells after removing the etoposide. Next, results of comet assay revealed decreased DNA repair after PES1-ablation. PES1-ablated cells were more sensitive to chemotherapeutic agents, which could be reversed by reconstitution with exogenous PES1. Furthermore, deletion of PES1 diminished steady and DNA damage-induced levels of nuclear RAD51. Our results uncover a potential role of PES1 in chemoresistance by regulating DNA damage response in colorectal cancer cells.

  3. Ginsenoside Rg3 regulates S-nitrosylation of the NLRP3 inflammasome via suppression of iNOS

    SciTech Connect (OSTI)

    Yoon, Sung-Jin; Park, Jun-Young; Choi, Song; Lee, Jin-Bong; Jung, Haiyoung; Kim, Tae-Don; Yoon, Suk Ran; Choi, Inpyo; Shim, Sungbo; Park, Young-Jun

    2015-08-07

    Ginsenoside Rg3, a specific biological effector, is well-known as a major bioactive ingredient of Panax ginseng. However, its role in the inflammasome activation process remains unclear. In this report, we demonstrate that ginsenosides 20(R)-Rg3 and 20(S)-Rg3 are capable of suppressing both lethal endotoxic shock and the S-nitrosylation of the NLRP3 inflammasome by inhibiting nitric oxide (NO) production through the regulation of inducible nitric oxide synthase (iNOS) expression. In response to lipopolysaccharide (LPS), the reducing effect of 20(S)-Rg3 and 20(R)-Rg3 on nitric oxide led to an increase in the survival time of mice after lethal endotoxin-induced shock, and excess levels of NO inhibited IL-1β production via the S-nitrosylation of the NLRP3 inflammasome. In addition, ginsenosides 20(R)-Rg3 and 20(S)-Rg3 had suppressive effects on the LPS- or UV-irradiation-induced reactive oxygen species (ROS) levels in macrophage and HaCaT cells and thereby prevented apoptosis of spleen cells in mice. Altogether, these results demonstrate that ginsenoside 20(R)-Rg3 and 20(S)-Rg3, a naturally occurring compound, might act as a dual therapeutic regulator for the treatment of inflammatory and oxidative stress-related diseases. - Highlights: • Ginsenosides Rg3 inhibits NO production through the regulation of iNOS expression. • Ginsenosides Rg3 inhibits the S-nitrosylation of the NLRP3 inflammasome. • Ginsenosides Rg3 suppress on the LPS- or UV-irradiation-induced ROS levels in cells.

  4. Nickel-regulated heart rate variability: The roles of oxidative stress and inflammation

    SciTech Connect (OSTI)

    Chuang, Hsiao-Chi; Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan ; Hsueh, Tzu-Wei; Chang, Chuen-Chau; Hwang, Jing-Shiang; Chuang, Kai-Jen; School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan ; Yan, Yuan-Horng; Department of Medical Research, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi City, Taiwan ; Cheng, Tsun-Jen; Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan

    2013-01-15

    Heart rate variability (HRV) has been reported to be a putative marker of cardiac autonomic imbalance caused by exposure to ambient particulate matter (PM). Our objective in this study was to determine the effects on HRV from exposure to nickel, an important chemical component of ambient PM that results in oxidative stress and inflammation. HRV data were collected for 72 h before lung exposure (baseline) and 72 h after intratracheal exposure (response) to nickel sulphate (NiSO{sub 4}; 526 ?g) in Wistar Kyoto (WKY) and spontaneously hypertensive (SH) rats. The antioxidant N-acetyl-L-cysteine (NAC) and the anti-inflammatory celecoxib were intraperitoneally injected to examine post-exposure oxidative and inflammatory responses. Self-controlled experiments examined the effects of NiSO{sub 4} exposure on average normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN) and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD); the resulting data were sequentially analysed using the generalised estimating equation model. HRV effects on NiSO{sub 4}-exposed SH rats were greater than those on NiSO{sub 4}-exposed WKY rats. After adjusted the HRV responses in the WKY rats as control, ANN and LnRMSSD were found to be quadratically increased over 72 h after exposure to NiSO{sub 4}. Both NAC and celecoxib mitigated the NiSO{sub 4}-induced alterations in HRV during the exposure period. The results suggest that concurrent Ni-induced oxidative stress and inflammatory responses play important roles in regulating HRV. These findings help bridge the gap between epidemiological and clinical studies on the plausible mechanisms of the cardiovascular consequences induced by chemical components in ambient PM. -- Highlights: ? To determine the effects on HRV from exposure to nickel. ? ANN and LnRMSSD were found to be quadratically increased after exposure to Ni. ? NAC and celecoxib mitigated the Ni-induced alterations in HRV. ? Ni-induced oxidative stress and inflammation play the roles in regulating HRV.

  5. Colorado geothermal institutional handbook: a user's guide of agencies regulations, permits, and aids for geothermal development

    SciTech Connect (OSTI)

    Coe, B.A.; Forman, N.A.

    1980-01-01

    The following are included: principal state agencies, applicable state legislation, applicable state regulations, local agencies and regulations, federal agencies and regulations, information sources, and agencies and individuals. (MHR)

  6. Genetic analysis of the regulation of TCH gene expression, Final Report

    SciTech Connect (OSTI)

    Braam, Janet

    2008-10-28

    The Arabidopsis TCH genes, originally isolated as a consequence of their upregulation in response to the mechanical stimulus of touch, are also upregulated by a variety of seemingly disparate environmental and hormonal stimuli. To gain insight into the complexities of TCH gene regulation, a number of approaches were taken. Regulatory elements responsible for regulation were identified and characteristics of the regulation were evaluated. Reporter genes were used to monitor expression localization and dynamics. Microarray analyses of genome-wide expression behavior indicated that touch-inducible gene expression is more widespread than generally appreciated. Identification of all touch-regulated genes shed light on the types of cellular processes that may be altered in response to mechanical stress perturbations. Expression of the TCH2 gene, also called CML24, encoding a calmodulin (CaM)-like (CML) protein, was evaluated. CML24 shares over 40% amino acid sequence identity with CaM, has 4 EF hands and undergoes a Ca2+-dependent change in migration rate through denaturing gel electrophoresis, indicating that CML24 binds Ca2+ and, as a consequence, undergoes conformational changes. CML24 expression occurs in all major organs and is induced from 2- to 15-fold in plants subjected to touch, darkness, heat, cold, hydrogen peroxide, abscisic acid (ABA) and indole-3-acetic acid. The putative CML24 regulatory region confers reporter expression at sites of predicted mechanical stress, in regions undergoing growth, in vascular tissues and various floral organs and in stomata, trichomes and hydathodes. CML24 underexpressing transgenics are resistant to ABA inhibition of germination and seedling growth, defective in long-day induction of flowering, and have enhanced tolerance to CoCl2, molybdic acid, ZnSO4 and MgCl2. These data present evidence that CML24 encodes a potential Ca2+ sensor that may function to enable responses to ABA, day length and presence of various salts. Further investigation of CML24 function and regulation led to the finding that CML24 has a critical role in nitric oxide regulation. Distinct tilling mutant alleles demonstrated that CML24 can act as a switch in the response to day length perception. Because of potential redundancy with the related CML23 gene, CML23 T-DNA insertion mutants were identified and characterized. Together, CML23 and CML24 impact the autonomous regulatory pathway of the transition to flowering. Nitric oxide levels are elevated in cml23/cml24 double mutants. Therefore, CML23 and CML24 are potential calcium sensors regulate nitric oxide accumulation. In collaboration with Drs. McCann and Carpita, fourier transform infrared spectroscopy (FTIR) was used to assess, verify and classify wall architectural changes that occur as a result of single XTH insertion mutations. Thirty-four homozygous mutant lines of Arabidopsis representing 21 members of the xyloglucan endotransglucosylase/hydrolase gene family provided a set of mutants to characterize. Kohonen networks classified cell wall architectures of xth mutant lines and previously characterized cell wall mutants. The xth mutants were found to have chemical changes in their cell walls not detectable as phenotypic growth and development changes, consistent with the existence of feed-back loops that modify wall composition in response to a life-long deficiency of a cell wall enzyme. To gain insight into the potential physiological relevance of the distinct members of the XTH family, GUS reporter fusion genes were constructed, and plants expressing these transgenes were characterized to reveal spatial and temporal patterns of expression. In addition, Genevestigator sources were mined for comprehensive and comparative XTH expression regulation analysis. These data revealed that the Arabidopsis XTHs are likely expressed in every developmental stage from seed germination through flowering. All organs showed XTH::GUS expression and most, if not all, are found to express multiple XTH::GUS genes suggesting that XTHs may contribute to morphogenesis at every d

  7. Nickel differentially regulates NFAT and NF-{kappa}B activation in T cell signaling

    SciTech Connect (OSTI)

    Saito, Rumiko; Hirakawa, Satoshi; Ohara, Hiroshi; Yasuda, Makoto; Yamazaki, Tomomi; Nishii, Shigeaki; Aiba, Setsuya

    2011-08-01

    Nickel is a potent hapten that induces contact hypersensitivity in human skin. While nickel induces the maturation of dendritic cells via NF-{kappa}B and p38 MAPK activation, it also exerts immunosuppressive effects on T cells through an unknown mechanism. To elucidate the molecular mechanisms of its effects on T cells, we examined the effects of NiCl{sub 2} on mRNA expression in human CD3+ T cells stimulated with CD3 and CD28 antibodies. Using a DNA microarray and Gene Ontology, we identified 70 up-regulated (including IL-1{beta}, IL-6 and IL-8) and 61 down-regulated (including IL-2, IL-4, IL-10 and IFN-{gamma}) immune responsive genes in NiCl{sub 2}-treated T cells. The DNA microarray results were verified using real-time PCR and a Bio-Plex{sup TM} suspension protein array. Suppression of IL-2 and IFN-{gamma} gene transcription by NiCl{sub 2} was also confirmed using Jurkat T cells transfected with IL-2 or IFN-{gamma} luciferase reporter genes. To explore the NiCl{sub 2}-regulated signaling pathway, we examined the binding activity of nuclear proteins to NFAT, AP-1, and NF-{kappa}B consensus sequences. NiCl{sub 2} significantly and dose-dependently suppressed NFAT- and AP-1-binding activity, but augmented NF-{kappa}B-binding activity. Moreover, NiCl{sub 2} decreased nuclear NFAT expression in stimulated T cells. Using Jurkat T cells stimulated with PMA/ionomycin, we demonstrated that NiCl{sub 2} significantly suppressed stimulation-evoked cytosolic Ca{sup 2+} increases, suggesting that NiCl{sub 2} regulates NFAT signals by acting as a blocker of Ca{sup 2+} release-activated Ca{sup 2+} (CRAC) channels. These data showed that NiCl{sub 2} decreases NFAT and increases NF-{kappa}B signaling in T cells. These results shed light on the effects of nickel on the molecular regulation of T cell signaling. - Graphical Abstract: Nickel suppresses stimulation-evoked cytosolic Ca{sup 2+} increase, which results in the suppression of NFAT signals. On the other hand, Ni rather stimulates NF-{kappa}B signaling. The effects of Nickel on these transcription factors modulate the expression of various immune related genes. Display Omitted Research Highlights: > Using DNA microarray and Gene Ontology, we identified 70 up-regulated and 61 down-regulated immune responsive genes in NiCl{sub 2}-treated T cells. > NiCl{sub 2} significantly suppressed the binding activity of nuclear proteins from stimulated T cells to NFAT and AP-1 consensus binding sites, while it rather augmented that from non-stimulated T cells to NF-{kappa}B. > NiCl{sub 2} significantly suppressed stimulation-evoked cytosolic Ca{sup 2+} increases, suggesting that NiCl{sub 2} regulates NFAT signals by acting as a blocker of CRAC channels.

  8. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    induced incandescence (LII) - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel Cycle Defense Waste Management Programs

  9. P70S6K 1 regulation of angiogenesis through VEGF and HIF-1{alpha} expression

    SciTech Connect (OSTI)

    Bian, Chuan-Xiu; Shi, Zhumei; Meng, Qiao; Jiang, Yue; Liu, Ling-Zhi; Jiang, Bing-Hua; Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107

    2010-07-30

    Research highlights: {yields} P70S6K1 regulates VEGF expression; {yields} P70S6K1 induces transcriptional activation through HIF-1{alpha} binding site; {yields} P70S6K1 regulates HIF-1{alpha}, but not HIF-1{beta} protein expression; {yields} P70S6K1 mediates tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression. -- Abstract: The 70 kDa ribosomal S6 kinase 1 (p70S6K1), a downstream target of phosphoinositide 3-kinase (PI3K) and ERK mitogen-activated protein kinase (MAPK), is an important regulator of cell cycle progression, and cell proliferation. Recent studies indicated an important role of p70S6K1 in PTEN-negative and AKT-overexpressing tumors. However, the mechanism of p70S6K1 in tumor angiogenesis remains to be elucidated. In this study, we specifically inhibited p70S6K1 activity in ovarian cancer cells using vector-based small interfering RNA (siRNA) against p70S6K1. We found that knockdown of p70S6K1 significantly decreased VEGF protein expression and VEGF transcriptional activation through the HIF-1{alpha} binding site at its enhancer region. The expression of p70S6K1 siRNA specifically inhibited HIF-1{alpha}, but not HIF-1{beta} protein expression. We also found that p70S6K1 down-regulation inhibited ovarian tumor growth and angiogenesis, and decreased cell proliferation and levels of VEGF and HIF-1{alpha} expression in tumor tissues. Our results suggest that p70S6K1 is required for tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression, providing a molecular mechanism of human ovarian cancer mediated by p70S6K1 signaling.

  10. Up-regulation of K{sub ir}2.1 by ER stress facilitates cell death of brain capillary endothelial cells

    SciTech Connect (OSTI)

    Kito, Hiroaki; Yamazaki, Daiju; Department of Biological Chemistry, Kyoto University, Graduate School of Pharmaceutical Sciences, Kyoto; Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Nagoya ; Ohya, Susumu; Yamamura, Hisao; Asai, Kiyofumi; Imaizumi, Yuji

    2011-07-29

    Highlights: {yields} We found that application of endoplasmic reticulum (ER) stress with tunicamycin to brain capillary endothelial cells (BCECs) induced cell death. {yields} The ER stress facilitated the expression of inward rectifier K{sup +} channel (K{sub ir}2.1) and induced sustained membrane hyperpolarization. {yields} The membrane hyperpolarization induced sustained Ca{sup 2+} entry through voltage-independent nonspecific cation channels and consequently facilitated cell death. {yields} The K{sub ir}2.1 up-regulation by ER stress is, at least in part, responsible for cell death of BCECs under pathological conditions. -- Abstract: Brain capillary endothelial cells (BCECs) form blood brain barrier (BBB) to maintain brain homeostasis. Cell turnover of BCECs by the balance of cell proliferation and cell death is critical for maintaining the integrity of BBB. Here we found that stimuli with tunicamycin, endoplasmic reticulum (ER) stress inducer, up-regulated inward rectifier K{sup +} channel (K{sub ir}2.1) and facilitated cell death in t-BBEC117, a cell line derived from bovine BCECs. The activation of K{sub ir} channels contributed to the establishment of deeply negative resting membrane potential in t-BBEC117. The deep resting membrane potential increased the resting intracellular Ca{sup 2+} concentration due to Ca{sup 2+} influx through non-selective cation channels and thereby partly but significantly regulated cell death in t-BBEC117. The present results suggest that the up-regulation of K{sub ir}2.1 is, at least in part, responsible for cell death/cell turnover of BCECs induced by a variety of cellular stresses, particularly ER stress, under pathological conditions.

  11. Incentive regulation of investor-owned nuclear power plants by public utility regulators. Revision 1

    SciTech Connect (OSTI)

    McKinney, M.D.; Seely, H.E.; Merritt, C.R.; Baker, D.C.

    1995-04-01

    The US Nuclear Regulatory Commission (NRC) periodically surveys the Federal Energy Regulatory Commission (FERC) and state regulatory commissions that regulate utility owners of nuclear power plants. The NRC is interested in identifying states that have established economic or performance incentive programs applicable to nuclear power plants, how the programs are being implemented, and in determining the financial impact of the programs on the utilities. The NRC interest stems from the fact that such programs have the potential to adversely affect the safety of nuclear power plants. The current report is an update of NUREG/CR-5975, Incentive Regulation of Investor-Owned Nuclear Power Plants by Public Utility Regulators, published in January 1993. The information in this report was obtained from interviews conducted with each state regulatory agency that administers an incentive program and each utility that owns at least 10% of an affected nuclear power plant. The agreements, orders, and settlements that form the basis for each incentive program were reviewed as required. The interviews and supporting documentation form the basis for the individual state reports describing the structure and financial impact of each incentive program.

  12. INPP4B-mediated tumor resistance is associated with modulation of glucose metabolism via hexokinase 2 regulation in laryngeal cancer cells

    SciTech Connect (OSTI)

    Min, Joong Won; Kim, Kwang Il; Kim, Hyun-Ah; Kim, Eun-Kyu; Noh, Woo Chul; Jeon, Hong Bae; Cho, Dong-Hyung; Oh, Jeong Su; Park, In-Chul; Hwang, Sang-Gu; Kim, Jae-Sung

    2013-10-11

    Highlights: HIF-1?-regulated INPP4B enhances glycolysis. INPP4B regulates aerobic glycolysis by inducing HK2 via Akt-mTOR pathway. Blockage of INPP4B and HK2 sensitizes radioresistant laryngeal cancer cells to radiation and anticancer drug. INPP4B is associated with HK2 in human laryngeal cancer tissues. -- Abstract: Inositol polyphosphate 4-phosphatase type II (INPP4B) was recently identified as a tumor resistance factor in laryngeal cancer cells. Herein, we show that INPP4B-mediated resistance is associated with increased glycolytic phenotype. INPP4B expression was induced by hypoxia and irradiation. Intriguingly, overexpression of INPP4B enhanced aerobic glycolysis. Of the glycolysis-regulatory genes, hexokinase 2 (HK2) was mainly regulated by INPP4B and this regulation was mediated through the Akt-mTOR pathway. Notably, codepletion of INPP4B and HK2 markedly sensitized radioresistant laryngeal cancer cells to irradiation or anticancer drug. Moreover, INPP4B was significantly associated with HK2 in human laryngeal cancer tissues. Therefore, these results suggest that INPP4B modulates aerobic glycolysis via HK2 regulation in radioresistant laryngeal cancer cells.

  13. Chapter 1 - Acquisition Regulations System | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    - Acquisition Regulations System Chapter 1 - Acquisition Regulations System PDF icon 1.1_Acquisition_Regulation_System_0.pdf PDF icon 1.2_Head_of_Contracting_Activity_(HCA)_Authority,_Functions,_and_Responsibilities.pdf PDF icon DOE Procurement Directors' Directory PDF icon Other DOE HCA List May 26 2015.pdf PDF icon 1.2_Attachment_Tables_I-IV_0.pdf PDF icon 1.3_Balanced_Scorecard_Assessment_Program_0.pdf PDF icon

  14. Code of Federal Regulations OCCUPATIONAL RADIATION PROTECTION | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy OCCUPATIONAL RADIATION PROTECTION Code of Federal Regulations OCCUPATIONAL RADIATION PROTECTION The rules in this part establish radiation protection standards, limits, and program requirements for protecting individuals from ionizing radiation resulting from the conduct of DOE activities. PDF icon Code of Federal Regulations OCCUPATIONAL RADIATION PROTECTION More Documents & Publications Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION Subpart A - General

  15. Contacts for the Deputy General Counsel for Litigation, Regulation and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Enforcement (GC-30) | Department of Energy Litigation, Regulation and Enforcement (GC-30) Contacts for the Deputy General Counsel for Litigation, Regulation and Enforcement (GC-30) Anne Harkavy, Deputy General Counsel for Litigation, Regulation, and Enforcement 202-586-8700 anne.harkavy@hq.doe.gov Margie L. Washington, Administrative Support Specialist 202-586-8700 202-586-3437 (fax) margie.washington@hq.doe.gov

  16. Natural Gas Regulation - Other Gas-Related Information Sources | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Natural Gas Regulation - Other Gas-Related Information Sources Natural Gas Regulation - Other Gas-Related Information Sources The single largest source of energy information available is the Department of Energy's Energy Information Administration (EIA). The EIA publishes extensive reports on natural gas and other energy sources. Domestic natural gas markets are regulated in part by the Federal Energy Regulatory Commission. The commission's chief area of concern is the interstate

  17. Building Codes and Regulations for Solar Water Heating Systems | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Codes and Regulations for Solar Water Heating Systems Building Codes and Regulations for Solar Water Heating Systems Photo Credit: iStockphoto Photo Credit: iStockphoto Before installing a solar water heating system, you should investigate local building codes, zoning ordinances, and subdivision covenants, as well as any special regulations pertaining to the site. You will probably need a building permit to install a solar energy system onto an existing building. Not every

  18. Harmonization of Federal and International Regulations | Department of

    Energy Savers [EERE]

    Energy Harmonization of Federal and International Regulations Harmonization of Federal and International Regulations Update of the U.S. Department of Transportation (DOT) Pipeline and Hazardous Materials Safety Administration (PHMSA). PDF icon Harmonization of Federal and International Regulations More Documents & Publications DOE-STD-5507-2013 Department of Transportation Pipeline and Hazardous Materials Safety Administration Activities FAQS Reference Guide - NNSA Package Certification

  19. Vehicle Technologies Office: Regulated Fleets | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Alternative Fuels » Vehicle Technologies Office: Regulated Fleets Vehicle Technologies Office: Regulated Fleets The Office of Energy Efficiency and Renewable Energy (EERE) manages several programs designed to fulfill the requirements of the original and amended versions of the Energy Policy Act of 1992 (EPAct) that regulate and guide specific types of fleets with the goal of reducing the United States' petroleum consumption. EERE's Vehicle Technologies Office (VTO) implements the EPAct

  20. Office of the Assistant General Counsel for Legislation, Regulation and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Efficiency | Department of Energy Legislation, Regulation and Energy Efficiency Office of the Assistant General Counsel for Legislation, Regulation and Energy Efficiency The Office of the Assistant General Counsel for Legislation, Regulation and Energy Efficiency (GC-33) provides legal support and advice on legislative matters throughout the Department. It participates in the development of DOE views on pending legislation, drafts DOE legislative proposals and supporting documents,

  1. Energy Department Revises NEPA Regulations to Improve Efficiency |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Revises NEPA Regulations to Improve Efficiency Energy Department Revises NEPA Regulations to Improve Efficiency October 3, 2011 - 12:39pm Addthis Washington, D.C. -- The U.S. Department of Energy will save time and money in its environmental reviews of many proposed energy projects under revised regulations approved September 27, 2011, to implement the National Environmental Policy Act (NEPA). The revisions focus on the Department's categorical exclusion provisions, and

  2. UAP56 is a novel interacting partner of Bcr in regulating vascular smooth muscle cell DNA synthesis

    SciTech Connect (OSTI)

    Sahni, Abha; Wang, Nadan; Alexis, Jeffrey D.

    2012-04-13

    Highlights: Black-Right-Pointing-Pointer UAP56 is an important regulator of DNA synthesis in vascular smooth muscle cells. Black-Right-Pointing-Pointer UAP56 binds to Bcr. Black-Right-Pointing-Pointer Interaction between Bcr and UAP56 is critical for Bcr induced DNA synthesis. -- Abstract: Bcr is a serine/threonine kinase that is a critical regulator of vascular smooth muscle cell inflammation and proliferation. We have previously demonstrated that Bcr acts in part via phosphorylation and inhibition of PPAR{gamma}. We have identified the RNA helicase UAP56 as another substrate of Bcr. In this report we demonstrate that knockdown of UAP56 blocks Bcr induced DNA synthesis in vascular smooth muscle cells (VSMC). We also found that over expression of Bcr increased the expression of cyclin E and decreased the expression of p27. Knockdown of UAP56 reversed the effect of Bcr on cyclin E and p27 expression. Furthermore, we found that Bcr binds to UAP56 and demonstrate that binding of UAP56 to Bcr is critical for Bcr induced DNA synthesis in VSMC. Our data identify UAP56 as an important binding partner of Bcr and a novel target for inhibiting vascular smooth muscle cell proliferation.

  3. 50 CFR 216 - Regulations Governing the Taking and Importing of...

    Open Energy Info (EERE)

    50 CFR 216 - Regulations Governing the Taking and Importing of Marine Mammals Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  4. A Utility Regulator's Guide to Data Access for Commercial Building...

    Broader source: Energy.gov (indexed) [DOE]

    use data to help commercial customers manage energy costs through building energy benchmarking. A Utility Regulator's Guide to Data Access for Commercial Building Energy...

  5. DOE's Program Regulating Liquefied Natural Gas Export Applications...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE's Program Regulating Liquefied Natural Gas Export Applications June 18, 2013 - 10:15am Addthis Statement of Christopher Smith, Acting Assistant Secretary for Fossil Energy ...

  6. USACE Engineer Regulation 1110-2-1454 Corps Responsibilities...

    Open Energy Info (EERE)

    USACE Engineer Regulation 1110-2-1454 Corps Responsibilities for Non-Federal Hydroelectric Power Development under the Federal Power Act Jump to: navigation, search OpenEI...

  7. Chapter 1 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    of the Wyoming Public Service Commission Regulations: Rules of Practice and Procedure Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  8. Title 22 California Code of Regulations | Open Energy Information

    Open Energy Info (EERE)

    Abstract Title 22 California Code of Regulations, current through August 7, 2014. Published NA Year Signed or Took Effect 2014 Legal Citation Title 22 California Code...

  9. Rules and Regulations of the Department of Transportation, Chapter...

    Open Energy Info (EERE)

    of Transportation, Chapter 13 - Access FacilitiesLegal Abstract This chapter sets forth Department of Transportation rules and regulations governing access facilities....

  10. TEEIC Laws and Regulations Applicable to Energy Transmission...

    Open Energy Info (EERE)

    TEEIC Laws and Regulations Applicable to Energy Transmission Development Website Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: TEEIC Laws and...

  11. Geothermal Regulations in Colorado - Land Ownership is the Key...

    Open Energy Info (EERE)

    Jump to: navigation, search OpenEI Reference LibraryAdd to library Conference Paper: Geothermal Regulations in Colorado - Land Ownership is the Key Abstract Geothermal resources in...

  12. Biomass Boiler and Furnace Emissions and Safety Regulations in...

    Open Energy Info (EERE)

    in the Northeast States Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Biomass Boiler and Furnace Emissions and Safety Regulations in the Northeast States Agency...

  13. Applicable EPA Regulations and Description Website | Open Energy...

    Open Energy Info (EERE)

    provides information regarding EPA regulations that apply to brownfields grants. Author Environmental Protection Agency Published EPA, Date Not Provided DOI Not Provided Check...

  14. A Regulator's Perspective on Interpretation of Performance and...

    Office of Environmental Management (EM)

    Interpretation of Performance and Risk Assessment Results A Regulator's Perspective on Interpretation of Performance and Risk Assessment Results Presentation from the 2015 Annual...

  15. Comments Received on Proposed Rulemaking for regulation implementing...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    section 216(h): Coordination of Federal Transmission Permitting on Federal Lands Comments Received on Proposed Rulemaking for regulation implementing section 216(h): Coordination ...

  16. EO 13211: Regulations That Significantly Affect Energy Supply...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Executive Order 13211 of May 18, 2001 Actions Concerning Regulations That Significantly ... present the related submission under Executive Order 12866 of September 30, 1993, or ...

  17. ADR Provisions in Federal Acquisition Regulation (FAR) | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation (FAR) More Documents & Publications Microsoft Word - ADR-Provisions-FederalAcquisitionReg.doc ACQUISITION LETTER DEAR Part 933 Microsoft Word - ACQUISITION LETTER.doc...

  18. Providing Minute-to-Minute Regulation from Wind Plants: Preprint

    SciTech Connect (OSTI)

    Kirby, B.; Milligan, M.; Ela, E.

    2010-10-01

    In this paper, we extend the previous analysis using time series data from existing wind plants, system loads, and regulation and energy markets.

  19. Control and regulation of modern distribution system, ForskEL...

    Open Energy Info (EERE)

    system, ForskEL (Smart Grid Project) Jump to: navigation, search Project Name Control and regulation of modern distribution system, ForskEL Country Denmark Coordinates...

  20. Distribution System Voltage Regulation by Distributed Energy Resources

    SciTech Connect (OSTI)

    Ceylan, Oguzhan; Liu, Guodong; Xu, Yan; Tomsovic, Kevin

    2014-01-01

    This paper proposes a control method to regulate voltages in 3 phase unbalanced electrical distribution systems. A constrained optimization problem to minimize voltage deviations and maximize distributed energy resource (DER) active power output is solved by harmony search algorithm. IEEE 13 Bus Distribution Test System was modified to test three different cases: a) only voltage regulator controlled system b) only DER controlled system and c) both voltage regulator and DER controlled system. The simulation results show that systems with both voltage regulators and DER control provide better voltage profile.

  1. Council on Environmental Quality (CEQ) NEPA Regulations: 40 CFR...

    Open Energy Info (EERE)

    LibraryAdd to library Legal Document- Secondary Legal SourceSecondary Legal Source: Council on Environmental Quality (CEQ) NEPA Regulations: 40 CFR 1500 - 1518Legal Author CEQ...

  2. Effective Regulatory Institutions: The Regulator's Role in the...

    Open Energy Info (EERE)

    Regulatory Institutions: The Regulator's Role in the Policy Process, Including Issues of Regulatory Independence Jump to: navigation, search Tool Summary LAUNCH TOOL Name:...

  3. Strategic Energy Management for Regulators of Ratepayer-Funded Programs

    SciTech Connect (OSTI)

    Existing Commercial Buildings Working Group

    2012-05-23

    Provides regulators with information on how to design and implement whole-building, continuous-improvement energy management programs for commercial buildings.

  4. Policy Flash 2013-23 Department of Energy Acquisition Regulation...

    Broader source: Energy.gov (indexed) [DOE]

    Attached is Policy Flash 2013-23 Department of Energy Acquisition Regulation (DEAR) Final Rule for changes to Parts 908, 945, 952, and 970 regarding Government Property. Questions...

  5. Activation of 200 MW refusegenerated CHP upward regulation effect...

    Open Energy Info (EERE)

    regulation effect Country Denmark Headquarters Location Thisted, Denmark Coordinates 56.959167, 8.703492 Loading map... "minzoom":false,"mappingservice":"googlemaps3","typ...

  6. Standards for Municipal Small Wind Regulations and Model Ordinance

    Broader source: Energy.gov [DOE]

    In July 2008, New Hampshire enacted legislation designed to prevent municipalities from adopting ordinances or regulations that place unreasonable limits on or hinder the performance of wind energy...

  7. Acquisition Guide Chapter 1.0- Acquisition Regulations System

    Broader source: Energy.gov [DOE]

    This section discusses the Civilian Agency Acquisition Council, agency acquisition regulations, deviations from the FAR and DEAR, ratification of unauthorized commitments, and Contracting Officers and their representatives.

  8. Re: Request for Information on Regulatory Burden of DOE Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon Re: Request for Information on Regulatory Burden of DOE Regulations More Documents & Publications Edison Electric Institute (EEI) Reducing Regulatory Burden RFI, 77 Fed. ...

  9. 5 CCR 1002-82 401 Certification Regulation | Open Energy Information

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 5 CCR 1002-82 401 Certification RegulationLegal Abstract Implementing regulations that...

  10. 10 V.S.A. Chapter 41 Regulation of Stream Flow | Open Energy...

    Open Energy Info (EERE)

    1 Regulation of Stream Flow Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 10 V.S.A. Chapter 41 Regulation of Stream...

  11. Title 10 Chapter 41 Regulation of Stream Flow | Open Energy Informatio...

    Open Energy Info (EERE)

    41 Regulation of Stream Flow Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Title 10 Chapter 41 Regulation of Stream...

  12. Emerging Latin American air quality regulation

    SciTech Connect (OSTI)

    Hosmer, A.W.; Vitale, E.M.; Guerrero, C.R.; Solorzano-Vincent, L.

    1998-12-31

    Latin America is the most urbanized region in the developing world. In recent years, significant economic growth has resulted in population migration from rural areas to urban centers, as well as in a substantial rise in the standard of living within the Region. These changes have impacted the air quality of Latin American countries as increased numbers of industrial facilities and motor vehicles release pollutants into the air. With the advent of new free trade agreements such as MERCOSUR and NAFTA, economic activity and associated pollutant levels can only be expected to continue to expand in the future. In order to address growing air pollution problems, many Latin America countries including Argentina, Brazil, Chile, Columbia, Costa Rica, and Mexico have passed, or will soon pass, new legislation to develop and strengthen their environmental frameworks with respect to air quality. As a first step toward understanding the impacts that this increased environmental regulation will have, this paper will examine the regulatory systems in six Latin American countries with respect to ambient air quality and for each of these countries: review a short history of the air quality problems within the country; outline the legal and institutional framework including key laws and implementing institutions; summarize in brief the current status of the country in terms of program development and implementation; and identify projected future trends. In addition, the paper will briefly review the international treaties that have bearing on Latin American air quality. Finally, the paper will conclude by identifying and exploring emerging trends in individual countries and the region as a whole.

  13. Protocadherin-7 induces bone metastasis of breast cancer

    SciTech Connect (OSTI)

    Li, Ai-Min; Tian, Ai-Xian; Zhang, Rui-Xue; Ge, Jie; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Sun, Xuan; Cao, Xu-Chen; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin

    2013-07-05

    Highlights: PCDH7 is overexpression in high bone metastatic MDA-MB-231 cells. PCDH7 is up-regulation in bone metastatic breast cancer tissues. Suppression of PCDH7 inhibits cell proliferation, migration, and invasion in vitro. PCDH7 induces breast cancer bone metastasis in vivo. -- Abstract: Breast cancer had a propensity to metastasize to bone, resulting in serious skeletal complications associated with poor outcome. Previous study showed that Protocadherin-7 (PCDH7) play an important role in brain metastatic breast cancer, however, the role of PCDH7 in bone metastatic breast cancer has never been explored. In the present study, we found that PCDH7 expression was up-regulation in bone metastatic breast cancer tissues by real-time PCR and immunohistochemistry assays. Furthermore, suppression of PCDH7 inhibits breast cancer cell proliferation, migration, and invasion in vitro by MTT, scratch, and transwell assays. Most importantly, overexpression of PCDH7 promotes breast cancer cell proliferation and invasion in vitro, and formation of bone metastasis in vivo. These data provide an important insight into the role of PCDH7 in bone metastasis of breast cancer.

  14. Mechanically stimulated bone cells secrete paracrine factors that regulate

    Office of Scientific and Technical Information (OSTI)

    osteoprogenitor recruitment, proliferation, and differentiation (Journal Article) | SciTech Connect Mechanically stimulated bone cells secrete paracrine factors that regulate osteoprogenitor recruitment, proliferation, and differentiation Citation Details In-Document Search Title: Mechanically stimulated bone cells secrete paracrine factors that regulate osteoprogenitor recruitment, proliferation, and differentiation Bone formation requires the recruitment, proliferation and osteogenic

  15. Federal Regulations for Natural Gas Imports and Exports | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Federal Regulations for Natural Gas Imports and Exports Federal Regulations for Natural Gas Imports and Exports PDF icon Section 3 of the Natural Gas Act More Documents & Publications Appendix B Patent and copyright cases 42 USC 17013

  16. Sox5 induces epithelial to mesenchymal transition by transactivation of Twist1

    SciTech Connect (OSTI)

    Pei, Xin-Hong; Lv, Xin-Quan; Li, Hui-Xiang

    2014-03-28

    Highlights: • Depletion of Sox5 inhibits breast cancer proliferation, migration, and invasion. • Sox5 transactivates Twist1 expression. • Sox5 induces epithelial to mesenchymal transition through transactivation of Twist1 expression. - Abstract: The epithelial to mesenchymal transition (EMT), a highly conserved cellular program, plays an important role in normal embryogenesis and cancer metastasis. Twist1, a master regulator of embryonic morphogenesis, is overexpressed in breast cancer and contributes to metastasis by promoting EMT. In exploring the mechanism underlying the increased Twist1 in breast cancer cells, we found that the transcription factor SRY (sex-determining region Y)-box 5(Sox5) is up-regulation in breast cancer cells and depletion of Sox5 inhibits breast cancer cell proliferation, migration, and invasion. Furthermore, depletion of Sox5 in breast cancer cells caused a dramatic decrease in Twist1 and chromosome immunoprecipitation assay showed that Sox5 can bind directly to the Twist1 promoter, suggesting that Sox5 transactivates Twist1 expression. We further demonstrated that knockdown of Sox5 up-regulated epithelial phenotype cell biomarker (E-cadherin) and down-regulated mesenchymal phenotype cell biomarkers (N-cadherin, Vimentin, and Fibronectin 1), resulting in suppression of EMT. Our study suggests that Sox5 transactivates Twist1 expression and plays an important role in the regulation of breast cancer progression.

  17. Ion-induced nuclear radiotherapy

    DOE Patents [OSTI]

    Horn, K.M.; Doyle, B.L.

    1996-08-20

    Ion-induced Nuclear Radiotherapy (INRT) is a technique for conducting radiosurgery and radiotherapy with a very high degree of control over the spatial extent of the irradiated volume and the delivered dose. Based upon the concept that low energy, ion induced atomic and nuclear reactions can be used to produce highly energetic reaction products at the site of a tumor, the INRT technique is implemented through the use of a conduit-needle or tube which conducts a low energy ion beam to a position above or within the intended treatment area. At the end of the conduit-needle or tube is a specially fabricated target which, only when struck by the ion beam, acts as a source of energetic radiation products. The inherent limitations in the energy, and therefore range, of the resulting reaction products limits the spatial extent of irradiation to a pre-defined volume about the point of reaction. Furthermore, since no damage is done to tissue outside this irradiated volume, the delivered dose may be made arbitrarily large. INRT may be used both as a point-source of radiation at the site of a small tumor, or as a topical bath of radiation to broad areas of diseased tissue. 25 figs.

  18. Ion-induced nuclear radiotherapy

    DOE Patents [OSTI]

    Horn, Kevin M.; Doyle, Barney L.

    1996-01-01

    Ion-induced Nuclear Radiotherapy (INRT) is a technique for conducting radiosurgery and radiotherapy with a very high degree of control over the spatial extent of the irradiated volume and the delivered dose. Based upon the concept that low energy, ion induced atomic and nuclear reactions can be used to produce highly energetic reaction products at the site of a tumor, the INRT technique is implemented through the use of a conduit-needle or tube which conducts a low energy ion beam to a position above or within the intended treatment area. At the end of the conduit-needle or tube is a specially fabricated target which, only when struck by the ion beam, acts as a source of energetic radiation products. The inherent limitations in the energy, and therefore range, of the resulting reaction products limits the spatial extent of irradiation to a pre-defined volume about the point of reaction. Furthermore, since no damage is done to tissue outside this irradiated volume, the delivered dose may be made arbitrarily large. INRT may be used both as a point-source of radiation at the site of a small tumor, or as a topical bath of radiation to broad areas of diseased tissue.

  19. Voltage Regulator Chip: Power Supplies on a Chip

    SciTech Connect (OSTI)

    2010-09-01

    ADEPT Project: CPES at Virginia Tech is finding ways to save real estate on a computer's motherboard that could be used for other critical functions. Every computer processor today contains a voltage regulator that automatically maintains a constant level of electricity entering the device. These regulators contain bulky components and take up about 30% of a computer's motherboard. CPES at Virginia Tech is developing a voltage regulator that uses semiconductors made of gallium nitride on silicon (GaN-on-Si) and high-frequency soft magnetic material. These materials are integrated on a small, 3D chip that can handle the same amount of power as traditional voltage regulators at 1/10 the size and with improved efficiency. The small size also frees up to 90% of the motherboard space occupied by current voltage regulators.

  20. Immunities from the antitrust laws for regulated entities

    SciTech Connect (OSTI)

    Jones, M.B.

    1985-05-30

    Just as regulation is an ever-present and crucial factor in most of the day-to-day operating decisions of regulated natural gas transmission and distribution companies, it is also an important factor to consider in assessing the application of the antitrust laws to the conduct of these regulated entities. The legal guidelines are far from clear and are evolving. However, as regulated entities find themselves in an ever more competitive environment, it becomes necessary for their legal counsel to monitor closely the developments in the law concerning immunities from the antitrust laws by virtue of regulation imposed by government. The author hopes that this article will provide a useful starting point for that analysis.

  1. SIRT1 inactivation induces inflammation through the dysregulation of autophagy in human THP-1 cells

    SciTech Connect (OSTI)

    Takeda-Watanabe, Ai; Kitada, Munehiro; Kanasaki, Keizo; Koya, Daisuke

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer SIRT1 inactivation decreases autophagy in THP-1 cell. Black-Right-Pointing-Pointer Inhibition of autophagy induces inflammation. Black-Right-Pointing-Pointer SIRT1 inactivation induces inflammation through NF-{kappa}B activation. Black-Right-Pointing-Pointer The p62/Sqstm1 accumulation by impairment of autophagy is related to NF-{kappa}B activation. Black-Right-Pointing-Pointer SIRT1 inactivation is involved in the activation of mTOR and decreased AMPK activation. -- Abstract: Inflammation plays a crucial role in atherosclerosis. Monocytes/macrophages are some of the cells involved in the inflammatory process in atherogenesis. Autophagy exerts a protective effect against cellular stresses like inflammation, and it is regulated by nutrient-sensing pathways. The nutrient-sensing pathway includes SIRT1, a NAD{sup +}-dependent histone deacetylase, which is implicated in the regulation of a variety of cellular processes including inflammation and autophagy. The mechanism through which the dysfunction of SIRT1 contributes to the regulation of inflammation in relation to autophagy in monocytes/macrophages is unclear. In the present study, we demonstrate that treatment with 2-[(2-Hydroxynaphthalen-1-ylmethylene)amino]-N-(1-phenethyl)benzamide (Sirtinol), a chemical inhibitor of SIRT1, induces the overexpression of inflammation-related genes such as tumor necrosis factor (TNF)-{alpha} and interleukin (IL)-6 through nuclear factor (NF)-{kappa}B signaling activation, which is associated with autophagy dysfunction, as shown through p62/Sqstm1 accumulation and decreased expression of light chain (LC) 3 II in THP-1 cells. The autophagy inhibitor, 3-methyladenine, also induces inflammation-related NF-{kappa}B activation. In p62/Sqstm1 knockdown cells, Sirtinol-induced inflammation through NF-{kappa}B activation is blocked. In addition, inhibition of SIRT1 is involved in the activation of the mammalian target of rapamycin (mTOR) pathway and is implicated in decreased 5 Prime -AMP activated kinase (AMPK) activation, leading to the impairment of autophagy. The mTOR inhibitor, rapamycin, abolishes Sirtinol-induced inflammation and NF-{kappa}B activation associated with p62/Sqstm1 accumulation. In summary, SIRT1 inactivation induces inflammation through NF-{kappa}B activation and dysregulates autophagy via nutrient-sensing pathways such as the mTOR and AMPK pathways, in THP-1 cells.

  2. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  3. A regulator`s perspective on NRC`s participation in the operations & maintenance committees

    SciTech Connect (OSTI)

    Wessman, R.H.

    1996-12-01

    As a regulator fairly new to the American Society of Mechanical Engineers (ASME) Operations and Maintenance (O&M) Committee process, the author does not have a personal historical perspective as do many of the longer-term, and highly respected, members of the O&M Committee. However, as Branch Chief of the Mechanical Engineering Branch, Division of Engineering, in the Office of Nuclear Reactor Regulation at the NRC for just over two years, he has responsibility for the regulatory agency`s review of licensee actions involving the products that come from the efforts of the O&M Committee, as well as responsibility for portions of the activities of interest to other ASME Code groups such as Section III, Section XI, and Qualification of Mechanical Equipment. As a result, the author has learned a great deal about the code process in a short time. Here he gives his perspectives on the process and provides a few thoughts on the direction for the future.

  4. A Test Bed for Self-regulating Distribution Systems: Modeling Intergrated Renewable Energy and Demand Response in the GridLAB-D/MATLAB Environment

    SciTech Connect (OSTI)

    Wang, Dan; de Wit, Braydon; Parkinson, Simon; Fuller, Jason C.; Chassin, David P.; Crawford, Curran; Djilali, Ned

    2012-01-16

    This paper discusses the development of a simulation test bed permitting the study of integrated renewable energy generators and controlled distributed heat pumps operating within distribution systems. The test bed is demonstrated in this paper by addressing the important issue of the self-regulating effect of consumer-owned air-source heat pumps on the variability induced by wind power integration, particularly when coupled with increased access to demand response realized through a centralized load control strategy.

  5. Regulation of MDA-MB-231 cell proliferation by GSK-3? involves epigenetic modifications under high glucose conditions

    SciTech Connect (OSTI)

    Gupta, Chanchal; Kaur, Jasmine; Tikoo, Kulbhushan

    2014-05-15

    Hyperglycemia is a critical risk factor for development and progression of breast cancer. We have recently reported that high glucose induces phosphorylation of histone H3 at Ser 10 as well as de-phosphorylation of GSK-3? at Ser 9 in MDA-MB-231 cells. Here, we elucidate the mechanism underlying hyperglycemia-induced proliferation in MDA-MB-231 breast cancer cells. We provide evidence that hyperglycemia led to increased DNA methylation and DNMT1 expression in MDA-MB-231 cells. High glucose condition led to significant increase in the expression of PCNA, cyclin D1 and decrease in the expression of PTPN 12, p21 and PTEN. It also induced hypermethylation of DNA at the promoter region of PTPN 12, whereas hypomethylation at Vimentin and Snail. Silencing of GSK-3? by siRNA prevented histone H3 phosphorylation and reduced DNMT1 expression. We show that chromatin obtained after immunoprecipitation with phospho-histone H3 was hypermethylated under high glucose condition, which indicates a cross-talk between DNA methylation and histone H3 phosphorylation. ChIP-qPCR analysis revealed up-regulation of DNMT1 and metastatic genes viz. Vimentin, Snail and MMP-7 by phospho-histone H3, which were down-regulated upon GSK-3? silencing. To the best of our knowledge, this is the first report which shows that interplay between GSK-3? activation, histone H3 phosphorylation and DNA methylation directs proliferation of breast cancer cells. - Highlights: High glucose induces phosphorylation of histone H3 and dephosphorylation of GSK-3?. Moreover, hyperglycemia also leads to increased DNA methylation in MDA-MB-231 cells. Inhibition of GSK-3? prevented histone H3 phosphorylation and reduced DNMT1 levels. Interplay exists between GSK-3?, histone H3 phosphorylation and DNA methylation.

  6. Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening

    SciTech Connect (OSTI)

    Xu, Hongbo; Chen, Xiaohong; Huang, Junwei [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Deng, Weiwei [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China)] [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Zhong, Qi [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Yue, Changli [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China)] [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China); Wang, Pingzhang, E-mail: wangpzh@bjmu.edu.cn [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China) [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China); Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Huang, Zhigang, E-mail: enthuangzhigang@sohu.com [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)

    2013-06-21

    Highlights: A novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors was defined. GPR65 was identified to induce the MMP3 expression. GPR65 mediated MMP induction under acidic conditions. AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. GPR65 overexpression can accelerate the invision of A549 cells. -- Abstract: Matrix metalloproteinases (MMPs) are over-expressed in nearly all cancers. To study novel regulatory factors of MMP expression in head and neck cancer (HNC), we screened a total of 636 candidate genes encoding putative human transmembrane proteins using MMP promoter reporter in a dual luciferase assay system. Three genes GPR65, AXL and TNFRSF10B dramatically activated the induction of MMP3 expression. The induction of MMP expression by GPR65 was further confirmed in A549 and/or FaDu cells. GPR65 mediated MMP induction under acidic conditions. The AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. Moreover, the A549 cells infected by recombinant adenovirus of GPR65 showed accelerated cell invasion. In conclusion, we validate that GPR65 is vital regulatory genes upstream of MMP3, and define a novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors.

  7. Extracellular Matrix-Regulated Gene Expression RequiresCooperation of SWI/SNF and Transcription Factors

    SciTech Connect (OSTI)

    Xu, Ren; Spencer, Virginia A.; Bissell, Mina J.

    2006-05-25

    Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation (ChIP) assays and mammary-specific genes as models, we show here that extracellular matrix (ECM) molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the {beta}- and ?-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/SNF complex, significantly reduced both {beta}- and ?-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. ChIP analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Coimmunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, C/EBP{beta}, and glucocorticoid receptor (GR). Thus, ECM- and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.

  8. Regulation of the human interleukin-2/interleukin-2 receptor system: A role for immunosuppression

    SciTech Connect (OSTI)

    Kaempfer, R.

    1994-12-31

    The strength of the cellular immune response is regulated to a large extent by the amount of interleukin-2 (IL-2) produced in response to a stimulus. The ability of lymphocytes and other cells to respond to IL-2 depends upon the expression of cell surface IL-2 receptors. Formation of a high-affinity IL-2 receptor is regulated primarily through induction of its {alpha} subunit, IL-2R{alpha}. Once formed, the IL-2R{alpha} chain turns over rapidly, rendering expression of high-affinity IL-2 receptors during the immune response dependent upon continuous activity of the IL-2R{alpha} gene. The induced expression of both human IL-2 and IL-2R{alpha} chains is sensitive to cell-mediated suppression by CD8 cells; depletion of CD8 cells leads to extensive superinduction. This coupled suppression of IL-2 and IL-2R{alpha} genes greatly increases the extend of control, and strongly limits the strength, of the signal transduced by this ligand/receptor system during an immune response. 29 refs., 3 figs.

  9. SENP1 inhibition induces apoptosis and growth arrest of multiple myeloma cells through modulation of NF-κB signaling

    SciTech Connect (OSTI)

    Xu, Jun; Sun, Hui-Yan; Xiao, Feng-Jun; Wang, Hua; Yang, Yang; Wang, Lu; Gao, Chun-Ji; Guo, Zi-Kuan; Wu, Chu-Tse; Wang, Li-Sheng

    2015-05-01

    SUMO/sentrin specific protease 1 (Senp1) is an important regulation protease in the protein sumoylation, which affects the cell cycle, proliferation and differentiation. The role of Senp1 mediated protein desumoylation in pathophysiological progression of multiple myeloma is unknown. In this study, we demonstrated that Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. Lentivirus-mediated Senp1 knockdown triggers apoptosis and reduces viability, proliferation and colony forming ability of MM cells. The NF-κB family members including P65 and inhibitor protein IkBα play important roles in regulation of MM cell survival and proliferation. We further demonstrated that Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation, leading to inactivation of NF-kB signaling in MM cells. These results delineate a key role for Senp1in IL-6 induced proliferation and survival of MM cells, suggesting it may be a potential new therapeutic target in MM. - Highlights: • Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. • Senp1 knockdown triggers apoptosis and reduces proliferation of MM cells. • Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation.

  10. Researchers modeling the potential for induced seismicity

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Researchers modeling the potential for induced seismicity Researchers modeling the potential for induced seismicity Researchers conducted modeling studies to better understand triggers of induced earthquake activity caused by injection of large volumes of liquid CO2. October 8, 2014 Summary of multiple injection well simulations after 10 years. Stars represent injection wells. Summary of multiple injection well simulations after 10 years. Stars represent injection wells. The team used the LANL

  11. C-MYC REGULATES SUBSTRATE OXIDATION PATTERNS DURING EARLY PRESSURE-OVERLOAD HYPERTROPHY

    SciTech Connect (OSTI)

    Ledee, Dolena R.; Smith, Lincoln; Kajimoto, Masaki; Bruce, Margaret; Isern, Nancy G.; Xu, Chun; Portman, Michael A.; Olson, Aaron

    2013-11-26

    Pressure overload cardiac hypertrophy alters substrate metabolism. Prior work showed that myocardial inactivation of c-Myc (Myc) attenuated hypertrophy and decreased expression of glycolytic genes after aortic constriction. Accordingly, we hypothesize that Myc regulates substrate preferences for the citric acid cycle during pressure overload hypertrophy from transverse aortic constriction (TAC) and that these metabolic changes impact cardiac function and growth. To test this hypothesis, we subjected FVB mice with cardiac specific, inducible Myc inactivation (MycKO-TAC) and non-transgenic littermates (Cont-TAC) to transverse aortic constriction (n=7/group). A separate group underwent sham surgery (Sham, n=5). After two weeks, function was measured in isolated working hearts along with substrate fractional contributions to the citric acid cycle by using perfusate with 13C labeled mixed fatty acids, lactate, ketones and unlabeled glucose and insulin. Western blots were used to evaluate metabolic enzymes. Cardiac function was similar between groups after TAC although +dP/dT and -dP/dT trended towards improvement in MycKO-TAC versus Cont-TAC. Compared to Sham, Cont-TAC had increased free fatty acid fractional contribution with a concurrent decrease in unlabeled (presumably glucose) contribution. Myc inactivation (MycKO-TAC) inhibited these metabolic changes. Hypertrophy in general increased protein levels of PKM2; however this change was not linked to Myc status. Protein post-translation modification by O-GlcNAc was significantly greater in Cont-TAC versus both Sham and MycKO-TAC. In conclusion, Myc regulates substrate utilization during early pressure overload hypertrophy. Our results show that the metabolic switch during hypertrophy is not necessary to maintain cardiac function, but it may be important mechanism to promote cardiomyocyte growth. Myc also regulates protein O-GlcNAcylation during hypertrophy.

  12. Experimental validation of advanced regulations for superconducting magnet cooling undergoing periodic heat loads

    SciTech Connect (OSTI)

    Lagier, B.; Rousset, B.; Hoa, C.; Bonnay, P.

    2014-01-29

    Superconducting magnets used in tokamaks undergo periodic heat load caused by cycling plasma operations inducing AC losses, neutrons fluxes and eddy currents in magnet structures. In the cryogenic system of JT60-SA tokamak, the Auxiliary Cold Box (ACB) distributes helium from the refrigerator to the cryogenic users and in particular to the superconducting magnets. ACB comprises a saturated helium bath with immersed heat exchangers, extracting heat from independent cooling loops. The supercritical helium flow in each cooling loop is driven by a cold circulator. In order to safely operate the refrigerator during plasma pulses, the interface between the ACB and the refrigerator shall be as stable as possible, with well-balanced bath inlet and outlet mass flows during cycling operation. The solution presented in this paper relies on a combination of regulations to smooth pulsed heat loads and to keep a constant refrigeration power during all the cycle. Two smoothing strategies are presented, both regulating the outlet mass flow of the bath: the first one using the bath as a thermal buffer and the second one storing energy in the loop by varying the cold circulator speed. The bath outlet mass flow is also controlled by an immersed resistive heater which enables a constant evaporation rate in the bath when power coming from the loops is decreasing. The refrigeration power is controlled so that the compensating power remains within an acceptable margin. Experimental validation is achieved using the HELIOS facility. This facility running at CEA Grenoble since 2010 is a scaled down model of the ACB bath and Central Solenoid magnet cooling loop of the JT60-SA tokamak. Test results show performances and robustness of the regulations.

  13. A primer on incentive regulation for electric utilities

    SciTech Connect (OSTI)

    Hill, L.J.

    1995-10-01

    In contemplating a regulatory approach, the challenge for regulators is to develop a model that provides incentives for utilities to engage in socially desirable behavior. In this primer, we provide guidance on this process by discussing (1) various models of economic regulation, (2) problems implementing these models, and (3) the types of incentives that various models of regulation provide electric utilities. We address five regulatory models in depth. They include cost-of-service regulation in which prudently incurred costs are reflected dollar-for-dollar in rates and four performance-based models: (1) price-cap regulation, in which ceilings are placed on the average price that a utility can charge its customers; (2) revenue-cap regulation, in which a ceiling is placed on revenues; (3) rate-of-return bandwidth regulation, in which a utility`s rates are adjusted if earnings fall outside a {open_quotes}band{close_quotes} around equity returns; and (4) targeted incentives, in which a utility is given incentives to improve specific components of its operations. The primary difference between cost-of-service and performance-based approaches is the latter sever the tie between costs and prices. A sixth, {open_quotes}mixed approach{close_quotes} combines two or more of the five basic ones. In the recent past, a common mixed approach has been to combine targeted incentives with cost-of-service regulation. A common example is utilities that are subject to cost-of-service regulation are given added incentives to increase the efficiency of troubled electric-generating units.

  14. Improved Laser Induced Breakdown Spectroscopy (LIBS) Elemental...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find More Like This Return to Search Improved Laser Induced Breakdown Spectroscopy (LIBS) ... NETL has developed a CO2 injection basin leakage detection system using miniaturized laser ...

  15. Interface Induced Carbonate Mineralization: A Fundamental Geochemical

    Office of Scientific and Technical Information (OSTI)

    Interface Induced Carbonate Mineralization: A Fundamental Geochemical Process Relevant to Carbon Sequestration Teng, H. Henry PI, The George Washington University PI, The George...

  16. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Print The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired...

  17. Induced Seismicity Impact | Open Energy Information

    Open Energy Info (EERE)

    Seismicity Impact Jump to: navigation, search Retrieved from "http:en.openei.orgwindex.php?titleInducedSeismicityImpact&oldid612409" Feedback Contact needs updating...

  18. Hydrogen Adsorption Induces Interlayer Carbon Bond Formation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydrogen Adsorption Induces Interlayer Carbon Bond Formation in Supported Few-Layer Graphene Friday, February 28, 2014 Among the allotropes of carbon, diamond has some of the most...

  19. State and federal regulation of OTEC plants in Hawaii

    SciTech Connect (OSTI)

    Keith, K.M.

    1980-09-01

    The advantages of ocean thermal energy conversion (OTEC) for Hawaii, its institutional support, projected contributions of OTEC in the future, and environmental concerns are discussed. Three experimental OTEC facilities in Hawaii are described, and the many regulations that must be observed and permits needed are described. Applicability of existing federal laws in regulating commercial-scale OTEC plants is examined, and applicable Coast Guard regulations and maritime laws are discussed briefly. Questions of state-federal relations, particularly regarding Hawaii's archipelagic claims and coastal zone, are addressed. (LEW)

  20. Enforcement Regulations and Directives - Worker Safety and Health |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Worker Safety and Health Enforcement Regulations and Directives - Worker Safety and Health Regulations 10 C.F.R. Part 851 - Worker Safety and Health Program; Final Rule 10 C.F.R. Part 850 - Chronic Beryllium Disease Prevention Program; Final Rule 29 C.F.R. Part 1910 - Occupational Safety and Health Standards (OSHA) 29 C.F.R. Part 1926 - Safety and Health Regulations for Construction (OSHA) 29 C.F.R. Part 1904 - Reporting and Reporting Occupational Injuries and Illnesses

  1. Industry Self-Regulation as a Means to Promote Nonproliferation

    SciTech Connect (OSTI)

    Hund, Gretchen; Elkhamri, Oksana O.

    2005-10-01

    Companies within numerous industries that have been “early adopters” of self-regulation concept, considering the environment and society alongside business issues, have realized several benefits and some competitive advantage while substantially improving their environmental performance. Given that proliferation prevention is also a public good, our premise is that the experience gained and lessons learned from the self-regulation initiative in other industries and more broadly in the arena of sustainable development provide a basis for examining the feasibility of developing self-regulation mechanisms applicable to industries involved with sensitive technologies (nuclear, radiological source, and other dual-use industries)

  2. Environmental regulation of coal mining, SMCRA`s second decade

    SciTech Connect (OSTI)

    McElfish, J.M.; Beier, A.E.

    1990-12-31

    This controversial book examines environmental regulation of coal mining in the United States over the 1979-1989 period. The Surface Mining Control and Reclamation Act of 1977 (SMCRA) established federal regulation of the environmental effects of coal mining. The permanent regulatory program implementing SMCRA was initiated in 1979. This book looks at the first ten years of SMCRA, seeking to draw lessons from experience that will improve regulation, so that the objectives of the law may be achieved in SMCRA`s second decade.

  3. Regulating for the long term: SMCRA and acid mine drainage

    SciTech Connect (OSTI)

    Shea, C.W.

    1995-12-31

    With the passage of the Surface Mining Control and Reclamation Act of 1977 (SMCRA), regulators and industry representatives expected to solve the problem of pollution of the Nation`s waterways caused by acidic discharges from coal mines. Eighteen years after the passage of SMCRA, hard issues of predicting, regulating and treating acid mine drainage remain. Acid mine drainage is most common in the coal seams of the Midwest and Appalachia: Pennsylvania, West Virginia, Maryland, Ohio, Illinois, and Tennessee. This article discusses regulation of coal mines and acid mine drainage for the long term.

  4. AG490 inhibits NFATc1 expression and STAT3 activation during RANKL induced osteoclastogenesis

    SciTech Connect (OSTI)

    Li, Chang-hong; Zhao, Jin-xia; Sun, Lin; Yao, Zhong-qiang; Deng, Xiao-li; Liu, Rui; Liu, Xiang-yuan

    2013-06-14

    Highlights: AG490 inhibits RANKL-induced osteoclastogenesis in RAW264.7 cells. AG490 affects cell proliferation and cell cycle distribution. AG490 reduces NFATc1 expression during RANKL-induced osteoclastogenesis. AG490 disrupts the activation of RANKL-mediated JAK2/STAT3 signaling pathway. STAT3 depletion partly mimics the effect of AG490 on RANKL-induced osteoclastogenesis. -- Abstract: Commonly, JAK/STAT relays cytokine signals for cell activation and proliferation, and recent studies have shown that the elevated expression of JAK/STAT is associated with the immune rejection of allografts and the inflammatory processes of autoimmune disease. However, the role which JAK2/STAT3 signaling plays in the receptor activator of nuclear factor-?B ligand (RANKL)-mediated osteoclastogenesis is unknown. In this study, we investigated the effects of AG490, specific JAK2 inhibitor, on osteoclast differentiation in vitro. AG490 significantly inhibited osteoclastogenesis in murine osteoclast precursor cell line RAW264.7 induced by RANKL. AG490 suppressed cell proliferation and delayed the G1 to S cell cycle transition. Furthermore, AG490 also suppressed the expression of nuclear factor of activated T cells (NFAT) c1 but not c-Fos in RAW264.7. Subsequently, we investigated various intracellular signaling components associated with osteoclastogenesis. AG490 had no effects on RANKL-induced activation of Akt, ERK1/2. Interestingly, AG490 partly inhibited RANKL-induced phosphorylation of Ser{sup 727} in STAT3. Additionally, down-regulation of STAT3 using siRNA resulted in suppression of TRAP, RANK and NFATc1 expression. In conclusion, we demonstrated that AG490 inhibited RANKL-induced osteoclastogenesis by suppressing NFATc1 production and cell proliferation via the STAT3 pathway. These results suggest that inhibition of JAK2 may be useful for the treatment of bone diseases characterized by excessive osteoclastogenesis.

  5. Global protein phosphorylation dynamics during deoxynivalenol-induced ribotoxic stress response in the macrophage

    SciTech Connect (OSTI)

    Pan, Xiao; Whitten, Douglas A.; Wu, Ming; Chan, Christina; Wilkerson, Curtis G.; Pestka, James J.

    2013-04-15

    Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium that commonly contaminates food, is capable of activating mononuclear phagocytes of the innate immune system via a process termed the ribotoxic stress response (RSR). To encapture global signaling events mediating RSR, we quantified the early temporal (≤ 30 min) phosphoproteome changes that occurred in RAW 264.7 murine macrophage during exposure to a toxicologically relevant concentration of DON (250 ng/mL). Large-scale phosphoproteomic analysis employing stable isotope labeling of amino acids in cell culture (SILAC) in conjunction with titanium dioxide chromatography revealed that DON significantly upregulated or downregulated phosphorylation of 188 proteins at both known and yet-to-be functionally characterized phosphosites. DON-induced RSR is extremely complex and goes far beyond its prior known capacity to inhibit translation and activate MAPKs. Transcriptional regulation was the main target during early DON-induced RSR, covering over 20% of the altered phosphoproteins as indicated by Gene Ontology annotation and including transcription factors/cofactors and epigenetic modulators. Other biological processes impacted included cell cycle, RNA processing, translation, ribosome biogenesis, monocyte differentiation and cytoskeleton organization. Some of these processes could be mediated by signaling networks involving MAPK-, NFκB-, AKT- and AMPK-linked pathways. Fuzzy c-means clustering revealed that DON-regulated phosphosites could be discretely classified with regard to the kinetics of phosphorylation/dephosphorylation. The cellular response networks identified provide a template for further exploration of the mechanisms of trichothecenemycotoxins and other ribotoxins, and ultimately, could contribute to improved mechanism-based human health risk assessment. - Highlights: ► Mycotoxin deoxynivalenol (DON) induces immunotoxicity via ribotoxic stress response. ► SILAC phosphoproteomics using TiO{sub 2} was applied to DON-treated RAW 264.7 cells. ► DON induces extensive protein phosphorylation changes involving 188 phosphoproteins. ► The main target of early DON-induced RSR is transcriptional regulation. ► Early DON-induced RSR is mediated by MAPK-, NFκB-, AKT- and AMPK-linked pathways.

  6. Activation of 200 MW refusegenerated CHP upward regulation effect...

    Open Energy Info (EERE)

    EU Smart Grid Projects Map1 Overview Waste CHP plants can be used in the electricity market for upward regulation by bypassing the steam turbine. The technical design for this...

  7. Y-12 and emerging environmental regulations in 1985

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    emerging environmental regulations in 1985 In Y-12's early days of its first mission, to separate Uranium 235 from natural uranium, the peak population hit 22,482 on August 21,...

  8. Title 14 CCR, Division 2, Chapter 4 - Development, Regulation...

    Open Energy Info (EERE)

    4 CCR, Division 2, Chapter 4 - Development, Regulation, and Conservation of Oil and Gas Resources Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  9. Structural Insight into the Regulation of the SNARE Assembly...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Insight into the Regulation of the SNARE Assembly by the Cell Polarity Protein Sro7 The cells that comprise many tissues are polarized, meaning that they have distinct 'sides' with...

  10. Ligand-specific regulation of the extracellular surface of a...

    Office of Scientific and Technical Information (OSTI)

    surface of a G-protein-coupled receptor Citation Details In-Document Search Title: Ligand-specific regulation of the extracellular surface of a G-protein-coupled ...

  11. Microsoft PowerPoint - Regulating Energy Commodities.ppt [Compatibilit...

    U.S. Energy Information Administration (EIA) Indexed Site

    Deanna Newcomb, MWE Regulatory & Compliance Analyst g y p y April 6, 2010 Where do you ... have a Compliance Program, do a review. y p g , The regulators are going to ask - What ...

  12. OAR 632-020 - Geothermal Regulations | Open Energy Information

    Open Energy Info (EERE)

    - Geothermal RegulationsLegal Published NA Year Signed or Took Effect 1972 Legal Citation OAR 632-020 (1972) DOI Not Provided Check for DOI availability: http:crossref.org...

  13. Staff Listing - Office of Regulation and International Engagement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DC 20585 Director of the Office of Regulation and International Engagement John A. Anderson, Director Room 3E-052 Telephone (202) 586-0521 FAX (202) 586-6050 Division of...

  14. Groundwater Quantity Regulation in Vermont: A Path Forward |...

    Open Energy Info (EERE)

    Groundwater Quantity Regulation in Vermont: A Path Forward Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- Secondary Legal SourceSecondary Legal...

  15. Issues in federal preemption of state appliance energy efficiency regulations

    SciTech Connect (OSTI)

    Fang, J.M.; Balistocky, S.; Schaefler, A.M.

    1982-12-01

    The findings and conclusions of the analysis of the various issues involved in the federal preemption of state regulations for the DOE no standard rule on covered appliances are summarized. The covered products are: refrigerators, refrigerator-freezers, freezers, clothes dryers, kitchen ranges and ovens, water heaters (excluding heat pump water heaters), room air conditioners, central air conditioners (excluding heat pumps), and furnaces. A detailed discussion of the rationale for the positions of groups offering comment for the record is presneted. The pertinent categories of state and local regulations and programs are explained, then detailed analysis is conducted on the covered products and regulations. Issues relating to the timing of preemption of state regulations are discussed, as well as issues relating to burden of proof, contents of petitions for exemptions from preemption, criteria for evaluating petitions, and procedural and other issues. (LEW)

  16. Rules and Regulations for the Management and Control of Designated...

    Open Energy Info (EERE)

    the Management and Control of Designated Ground Water Jump to: navigation, search OpenEI Reference LibraryAdd to library Reference: Rules and Regulations for the Management and...

  17. Title 40 Code of Federal Regulations Chapter I, Subchapter C...

    Open Energy Info (EERE)

    Page Edit with form History Title 40 Code of Federal Regulations Chapter I, Subchapter C - Air Programs Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal...

  18. Arrangement between the Office for Nuclear Regulation of Great...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Arrangement between the Office for Nuclear Regulation of Great Britain and the United States Department of Energy for the Exchange of Information and Co-operation in the Area of ...

  19. Oak Ridge EM Program Collaborates with Regulators on Groundwater Strategy

    Broader source: Energy.gov [DOE]

    OAK RIDGE, Tenn. – The Oak Ridge EM program has joined state and federal regulators in a series of workshops to address contaminated groundwater on the Oak Ridge Reservation.

  20. Elucidating the Molecular Basis and Regulation of Chromium (VI...

    Office of Scientific and Technical Information (OSTI)

    by Shewanella oneidensis MR-1. We have identified and characterized (in the case of DNA-binding response regulator SO2426 and a putative azoreductase SO3585) the genes and ...

  1. Contacts for the Assistant General Counsel for Legislation, Regulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Daniel Cohen, Assistant General Counsel for Legislation, Regulation, & Energy Efficiency 202-586-9523 daniel.cohen@hq.doe.gov Catherine Goshe, Attorney-Adviser 202-586-7053 Preeti ...

  2. Current and proposed regulations for salt water disposal wells

    SciTech Connect (OSTI)

    Moody, T.

    1994-09-01

    In recent years, all aspects of hydrocarbon exploration and production (E & P) activities have drawn closer scrutiny in terms of existing and potential impairment of the environment. In addition to drilling, production, and transportation activities, the USEPA has focused on the nature of E & P generated wastes, and the subsequent management of both hazardous and nonhazardous E & P wastes. Approximately 98% of all of the volume of wastes generated by E & P activities is salt water associated with the recovery of hydrocarbons. By far the majority of this waste is disposed of in class II salt water disposal wells. Due to the tremendous volume of salt water generated, the USEPA continues to reevaluate the federal class II salt water injection well program, offering comments, revising its interpretation of existing regulations, and promulgating new regulations. The purpose of the presentation will be to provide a review of existing class II federal regulations, and to provide an overview of potential or newly promulgated regulations.

  3. Current and proposed regulations for salt-water disposal wells

    SciTech Connect (OSTI)

    Moody, T.

    1994-12-31

    In recent years, all aspects of hydrocarbon exploration and production (E&P) activities have drawn closer scrutiny in terms of existing and potential impairment of the environment. In addition to drilling, production, and transportation activities, the United States Environmental Protection Agency (USEPA) has focused on the nature of E&P wastes. Approximately 98% of the volume of wastes generated by E&P activities is salt water associated with the recovery of hydrocarbons. By far the majority of this waste is reinjected in Class II wells as a nonhazardous waste. Due to the tremendous volume of salt water disposed of in Class II injection wells, the USEPA continues to reevaluate the Federal salt-water injection well program, offering comments, revising its interpretation of existing regulations, and promulgating new regulations. The purpose of this paper is to provide a review of existing Federal Class II injection well regulations and to provide an overview of potential of newly promulgated regulations.

  4. Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells

    SciTech Connect (OSTI)

    Sassoli, Chiara; Nosi, Daniele; Tani, Alessia; Chellini, Flaminia; Mazzanti, Benedetta; Quercioli, Franco; Zecchi-Orlandini, Sandra; Formigli, Lucia

    2014-05-01

    Recent studies indicate that mesenchymal stromal cell (MSC) transplantation improves healing of injured and diseased skeletal muscle, although the mechanisms of benefit are poorly understood. In the present study, we investigated whether MSCs and/or their trophic factors were able to regulate matrix metalloproteinase (MMP) expression and activity in different cells of the muscle tissue. MSCs in co-culture with C2C12 cells or their conditioned medium (MSC-CM) up-regulated MMP-2 and MMP-9 expression and function in the myoblastic cells; these effects were concomitant with the down-regulation of the tissue inhibitor of metalloproteinases (TIMP)-1 and -2 and with increased cell motility. In the single muscle fiber experiments, MSC-CM administration increased MMP-2/9 expression in Pax-7{sup +} satellite cells and stimulated their mobilization, differentiation and fusion. The anti-fibrotic properties of MSC-CM involved also the regulation of MMPs by skeletal fibroblasts and the inhibition of their differentiation into myofibroblasts. The treatment with SB-3CT, a potent MMP inhibitor, prevented in these cells, the decrease of ?-smooth actin and type-I collagen expression induced by MSC-CM, suggesting that MSC-CM could attenuate the fibrogenic response through mechanisms mediated by MMPs. Our results indicate that growth factors and cytokines released by these cells may modulate the fibrotic response and improve the endogenous mechanisms of muscle repair/regeneration. - Highlights: MSC-CM contains paracrine factors that up-regulate MMP expression and function in different skeletal muscle cells. MSC-CM promotes myoblast and satellite cell migration, proliferation and differentiation. MSC-CM negatively interferes with fibroblast-myoblast transition in primary skeletal fibroblasts. Paracrine factors from MSCs modulate the fibrotic response and improve the endogenous mechanisms of muscle regeneration.

  5. Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy PART 835-OCCUPATIONAL RADIATION PROTECTION Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION General. The rules in this part establish radiation protection standards, limits, and program requirements for protecting individuals from ionizing radiation resulting from the conduct of DOE activities. PDF icon Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION More Documents & Publications Order Module--NNSA OCCUPATIONAL RADIATION

  6. Comments Received on Proposed Rulemaking for regulation implementing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    section 216(h): Coordination of Federal Transmission Permitting on Federal Lands | Department of Energy Received on Proposed Rulemaking for regulation implementing section 216(h): Coordination of Federal Transmission Permitting on Federal Lands Comments Received on Proposed Rulemaking for regulation implementing section 216(h): Coordination of Federal Transmission Permitting on Federal Lands Section 1221(a) of EPAct 2005, which added section 216(h) to the Federal Power Act, stated that the

  7. Method to determine transcriptional regulation pathways in organisms

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Method to determine transcriptional regulation pathways in organisms Citation Details In-Document Search Title: Method to determine transcriptional regulation pathways in organisms × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy science and

  8. Code of Federal Regulations Nuclear Activities | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Code of Federal Regulations Nuclear Activities Code of Federal Regulations Nuclear Activities April 24,2010 This part sets forth the procedures to govern the conduct of persons involved in DOE nuclear activities and, in particular, to achieve compliance with the DOE Nuclear Safety Requirements by all persons subject to those requirements. 10 C.F.R. 820, Procedural Rules for DOE Nuclear Activities, sets forth the procedures to implement the provisions of the Price-Anderson Amendments Act of 1988

  9. Collegiate Wind Competition Rules and Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Collegiate Wind Competition Rules and Regulations Collegiate Wind Competition Rules and Regulations The competition challenges interdisciplinary teams of undergraduate students from a variety of academic programs to offer unique solutions to complex wind-energy-related problems. To fulfill the requirements, each team must perform the following multifaceted tasks: Develop and deliver a market-research-supported business plan that shapes the design and development of the team's turbine and load

  10. DOE Proposes Regulations for Loan Guarantee Program | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations for Loan Guarantee Program DOE Proposes Regulations for Loan Guarantee Program May 10, 2007 - 12:45pm Addthis Program to spur clean, innovative energy technologies WASHINGTON, DC - The U.S. Department of Energy (DOE) today issued a Notice of Proposed Rulemaking (NOPR) for its Loan Guarantee program, which will help spur investment in projects that employ new, clean energy technologies. Projects within DOE's Loan Guarantee program will help sustain economic growth, yield environmental

  11. QB1 - Stochastic Gene Regulation (Conference) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Conference: QB1 - Stochastic Gene Regulation Citation Details In-Document Search Title: QB1 - Stochastic Gene Regulation Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present in the cell - Random motion and competition between reactants, Low copy, quantization of reactants, Upstream processes; (2) Fluctuations may be very important - Cell-to-cell variability, Cell fate decisions (switches), Signal amplification or damping, stochastic resonances; and (3) Some tools

  12. RNA-guided transcriptional regulation (Patent) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Patent: RNA-guided transcriptional regulation Citation Details In-Document Search Title: RNA-guided transcriptional regulation Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided

  13. New methods for tightly regulated gene expression and highly efficient

    Office of Scientific and Technical Information (OSTI)

    chromosomal integration of cloned genes for Methanosarcina species (Journal Article) | SciTech Connect New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species Citation Details In-Document Search Title: New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species A highly efficient method for chromosomal integration of cloned DNA into

  14. Regulations.gov - Proposed Rule Document | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations.gov - Proposed Rule Document Regulations.gov - Proposed Rule Document In response to your phone call and e-mail below the proposed rule that you reference below regarding energy efficiency standards for manufactured housing has not been issued yet by the Department of Energy (DOE) and therefore we are still in the process of drafting a proposed rule and any necessary definitions. PDF icon Proposed Rule Email More Documents & Publications ISSUANCE 2015-02-04: Energy Conservation

  15. Department of Energy Acquisition Regulation (DEAR) Subpart 903.9:

    Energy Savers [EERE]

    Whistleblower Protections for Contractor Employees | Department of Energy Department of Energy Acquisition Regulation (DEAR) Subpart 903.9: Whistleblower Protections for Contractor Employees Department of Energy Acquisition Regulation (DEAR) Subpart 903.9: Whistleblower Protections for Contractor Employees Stakeholders: DOE Contractor employees Scope: This subpart of DEAR implements the DOE Contractor Employee Protection Program as set forth at 10 CFR part 708. This establishes the criteria

  16. Medical and biofuel advances possible with new gene regulation tool

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Medical and biofuel advances possible with new gene regulation tool Medical and biofuel advances possible with new gene regulation tool The key is a tunable switch made from a small non-coding RNA molecule that could have value for medical and even biofuel production purposes. August 20, 2015 Scientists at Los Alamos National Laboratory have manufactured molecular "dimmer" switches to control cellular metabolism with exquisite precision. The research has potential widespread

  17. NNSA Updates Export Control Regulation | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration Updates Export Control Regulation February 24, 2015 WASHINGTON, D.C. - The National Nuclear Security Administration (NNSA) updated the Federal rule (10 CFR Part 810, or Part 810) that regulates the export of unclassified nuclear technology and assistance. The final rule was published in the Federal Register on Feb. 23 and will go into effect on March 25, 2015. Part 810 enables civil nuclear trade by ensuring that nuclear technology and assistance exported from the United

  18. Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Subpart A - General Provisions | Department of Energy PART 835-OCCUPATIONAL RADIATION PROTECTION Subpart A - General Provisions Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION Subpart A - General Provisions The rules in this part establish radiation protection standards, limits, and program requirements for protecting individuals from ionizing radiation resulting from the conduct of DOE activities. PDF icon Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION

  19. Synthesis of segmented silica rods by growth temperature regulation

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect segmented silica rods by growth temperature regulation Citation Details In-Document Search Title: Synthesis of segmented silica rods by growth temperature regulation Authors: Sharma, Jaswinder K [1] ; Datskos, Panos G [1] + Show Author Affiliations ORNL Publication Date: 2014-01-01 OSTI Identifier: 1115377 DOE Contract Number: DE-AC05-00OR22725 Resource Type: Journal Article Resource Relation: Journal Name: Angewandte Chemie International Edition; Journal

  20. A Simpler, Smarter and More Cost Effective Approach to Regulation |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy A Simpler, Smarter and More Cost Effective Approach to Regulation A Simpler, Smarter and More Cost Effective Approach to Regulation May 26, 2011 - 9:17am Addthis Sean Lev Sean Lev Acting General Counsel & Deputy General Counsel, Environment & Nuclear Programs What does this mean for me? The Department projects a more than 90% reduction in the paperwork burden imposed on recipients of the Department of Energy's financial assistance. The Department is also working

  1. California Regulations on Renewble Hydrogen and Low Carbon Technologies |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Regulations on Renewble Hydrogen and Low Carbon Technologies California Regulations on Renewble Hydrogen and Low Carbon Technologies Presentation at the Renewable Hydrogen Workshop, Nov. 16, 2009, in Palm Springs, CA PDF icon renewable_hydrogen_workshop_nov16_achtelik.pdf More Documents & Publications Transportation and Stationary Power Integration Workshop: A California Perspective Vision for Rollout of Fuel Cell Vehicles and Hydrogen Fuel Stations State of the

  2. Compensated gain control circuit for buck regulator command charge circuit

    DOE Patents [OSTI]

    Barrett, David M.

    1996-01-01

    A buck regulator command charge circuit includes a compensated-gain control signal for compensating for changes in the component values in order to achieve optimal voltage regulation. The compensated-gain control circuit includes an automatic-gain control circuit for generating a variable-gain control signal. The automatic-gain control circuit is formed of a precision rectifier circuit, a filter network, an error amplifier, and an integrator circuit.

  3. Compensated gain control circuit for buck regulator command charge circuit

    DOE Patents [OSTI]

    Barrett, D.M.

    1996-11-05

    A buck regulator command charge circuit includes a compensated-gain control signal for compensating for changes in the component values in order to achieve optimal voltage regulation. The compensated-gain control circuit includes an automatic-gain control circuit for generating a variable-gain control signal. The automatic-gain control circuit is formed of a precision rectifier circuit, a filter network, an error amplifier, and an integrator circuit. 5 figs.

  4. Interface Induced Carbonate Mineralization: A Fundamental Geochemical

    Office of Scientific and Technical Information (OSTI)

    Process Relevant to Carbon Sequestration (Technical Report) | SciTech Connect Interface Induced Carbonate Mineralization: A Fundamental Geochemical Process Relevant to Carbon Sequestration Citation Details In-Document Search Title: Interface Induced Carbonate Mineralization: A Fundamental Geochemical Process Relevant to Carbon Sequestration Mica, biotite, muscovite, diopside, tremolite, ultramafic rock, hematite, Ca-Mg-carbonate, calcite, aragonite, dolomite, crystal nucleation,

  5. Solvent-induced forces in protein folding

    SciTech Connect (OSTI)

    Ben-Naim, A. (Hebrew Univ., Jerusalem (Israel))

    1990-08-23

    The solvent-induced forces between various groups on the protein are examined. It is found that the intramolecular hydrophilic forces are likely to be the strongest forces mediated through the solvent. It is argued that these are probably the most important solvent-induced driving forces in the process of protein folding.

  6. Induced topological pressure for topological dynamical systems

    SciTech Connect (OSTI)

    Xing, Zhitao; Chen, Ercai

    2015-02-15

    In this paper, inspired by the article [J. Jaerisch et al., Stochastics Dyn. 14, 1350016, pp. 1-30 (2014)], we introduce the induced topological pressure for a topological dynamical system. In particular, we prove a variational principle for the induced topological pressure.

  7. Rab proteins: The key regulators of intracellular vesicle transport

    SciTech Connect (OSTI)

    Bhuin, Tanmay; Roy, Jagat Kumar

    2014-10-15

    Vesicular/membrane trafficking essentially regulates the compartmentalization and abundance of proteins within the cells and contributes in many signalling pathways. This membrane transport in eukaryotic cells is a complex process regulated by a large and diverse array of proteins. A large group of monomeric small GTPases; the Rabs are essential components of this membrane trafficking route. Most of the Rabs are ubiquitously expressed proteins and have been implicated in vesicle formation, vesicle motility/delivery along cytoskeleton elements and docking/fusion at target membranes through the recruitment of effectors. Functional impairments of Rabs affecting transport pathways manifest different diseases. Rab functions are accompanied by cyclical activation and inactivation of GTP-bound and GDP-bound forms between the cytosol and membranes which is regulated by upstream regulators. Rab proteins are characterized by their distinct sub-cellular localization and regulate a wide variety of endocytic, transcytic and exocytic transport pathways. Mutations of Rabs affect cell growth, motility and other biological processes. - Highlights: Rab proteins regulate different signalling pathways. Deregulation of Rabs is the fundamental causes of a variety of human diseases. This paper gives potential directions in developing therapeutic targets. This paper also gives ample directions for modulating pathways central to normal physiology. These are the huge challenges for drug discovery and delivery in near future.

  8. Hazardous waste identification: A guide to changing regulations

    SciTech Connect (OSTI)

    Stults, R.G. )

    1993-03-01

    The Resource Conservation and Recovery Act (RCRA) was enacting in 1976 and amended in 1984 by the Hazardous and Solid Waste Amendments (HSWA). Since then, federal regulations have generated a profusion of terms to identify and describe hazardous wastes. Regulations that5 define and govern management of hazardous wastes are codified in Title 40 of the code of Federal Regulations, Protection of the environment''. Title 40 regulations are divided into chapters, subchapters and parts. To be defined as hazardous, a waste must satisfy the definition of solid waste any discharged material not specifically excluded from regulation or granted a regulatory variance by the EPA Administrator. Some wastes and other materials have been identified as non-hazardous and are listed in 40 CFR 261.4(a) and 261.4(b). Certain wastes that satisfy the definition of hazardous waste nevertheless are excluded from regulation as hazardous if they meet specific criteria. Definitions and criteria for their exclusion are found in 40 CFR 261.4(c)-(f) and 40 CFR 261.5.

  9. Consumer attitudes toward potentially restrictive energy conservation regulations

    SciTech Connect (OSTI)

    Merfeld, M.K.

    1984-01-01

    A sample of 893 Oregon individuals from a Western Regional Agricultural Experiment Station Project (W-159 Consequences of Energy Conservation Policies for Western Region Households) was used to analyze consumer attitudes toward potentially restrictive energy conservation regulations. Belief in the seriousness of the energy problem, a psychological measure of internal control (internality), and eight socio-demographic characteristics were examined in relation to consumer attitudes toward these regulations. Those who did not believe in the seriousness of the energy problem were over 50 years of age, male, had less than a college degree, and opposed mandatory energy conservation regulations related to home thermostat settings. Homeowners opposed the regulation requiring their homes to pass an energy audit. Opposition to regulations appeared to be related only to an individual's perception of the consequences of the regulation, and not to belief in the enregy problem or locus of control. Those with low internality scores included females, rural residents, respondents over 50, and those with less than a college degree.

  10. Global identification of genes regulated by estrogen signaling and demethylation in MCF-7 breast cancer cells

    SciTech Connect (OSTI)

    Putnik, Milica; Zhao, Chunyan; Gustafsson, Jan-Ake; Department of Biology and Biochemistry, Science and Engineering Research Center Bldg, University of Houston, Houston, TX 77204-5056 ; Dahlman-Wright, Karin

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer Estrogen signaling and demethylation can both control gene expression in breast cancers. Black-Right-Pointing-Pointer Cross-talk between these mechanisms is investigated in human MCF-7 breast cancer cells. Black-Right-Pointing-Pointer 137 genes are influenced by both 17{beta}-estradiol and demethylating agent 5-aza-2 Prime -deoxycytidine. Black-Right-Pointing-Pointer A set of genes is identified as targets of both estrogen signaling and demethylation. Black-Right-Pointing-Pointer There is no direct molecular interplay of mediators of estrogen and epigenetic signaling. -- Abstract: Estrogen signaling and epigenetic modifications, in particular DNA methylation, are involved in regulation of gene expression in breast cancers. Here we investigated a potential regulatory cross-talk between these two pathways by identifying their common target genes and exploring underlying molecular mechanisms in human MCF-7 breast cancer cells. Gene expression profiling revealed that the expression of approximately 140 genes was influenced by both 17{beta}-estradiol (E2) and a demethylating agent 5-aza-2 Prime -deoxycytidine (DAC). Gene ontology (GO) analysis suggests that these genes are involved in intracellular signaling cascades, regulation of cell proliferation and apoptosis. Based on previously reported association with breast cancer, estrogen signaling and/or DNA methylation, CpG island prediction and GO analysis, we selected six genes (BTG3, FHL2, PMAIP1, BTG2, CDKN1A and TGFB2) for further analysis. Tamoxifen reverses the effect of E2 on the expression of all selected genes, suggesting that they are direct targets of estrogen receptor. Furthermore, DAC treatment reactivates the expression of all selected genes in a dose-dependent manner. Promoter CpG island methylation status analysis revealed that only the promoters of BTG3 and FHL2 genes are methylated, with DAC inducing demethylation, suggesting DNA methylation directs repression of these genes in MCF-7 cells. In a further analysis of the potential interplay between estrogen signaling and DNA methylation, E2 treatment showed no effect on the methylation status of these promoters. Additionally, we show that the ER{alpha} recruitment occurs at the FHL2 promoter in an E2- and DAC-independent fashion. In conclusion, we identified a set of genes regulated by both estrogen signaling and DNA methylation. However, our data does not support a direct molecular interplay of mediators of estrogen and epigenetic signaling at promoters of regulated genes.

  11. Arsenite evokes IL-6 secretion, autocrine regulation of STAT3 signaling, and miR-21 expression, processes involved in the EMT and malignant transformation of human bronchial epithelial cells

    SciTech Connect (OSTI)

    Luo, Fei; Xu, Yuan; Ling, Min; Zhao, Yue; Xu, Wenchao; Liang, Xiao; Jiang, Rongrong; Wang, Bairu; Bian, Qian; Liu, Qizhan

    2013-11-15

    Arsenite is an established human carcinogen, and arsenite-induced inflammation contributes to malignant transformation of cells, but the molecular mechanisms by which cancers are produced remain to be established. The present results showed that, evoked by arsenite, secretion of interleukin-6 (IL-6), a pro-inflammatory cytokine, led to the activation of STAT3, a transcription activator, and to increased levels of a microRNA, miR-21. Blocking IL-6 with anti-IL-6 antibody and inhibiting STAT3 activation reduced miR-21 expression. For human bronchial epithelial cells, cultured in the presence of anti-IL-6 antibody for 3 days, the arsenite-induced EMT and malignant transformation were reversed. Thus, IL-6, acting on STAT3 signaling, which up-regulates miR-21in an autocrine manner, contributes to the EMT induced by arsenite. These data define a link from inflammation to EMT in the arsenite-induced malignant transformation of HBE cells. This link, mediated through miRNAs, establishes a mechanism for arsenite-induced lung carcinogenesis. - Highlights: Arsenite evokes IL-6 secretion. IL-6 autocrine mediates STAT3 signaling and up-regulates miR-21expression. Inflammation is involved in arsenite-induced EMT.

  12. O.A.R. 340-045 - Regulations Pertaining to NPDES and WPCF Permits...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: O.A.R. 340-045 - Regulations Pertaining to NPDES and WPCF PermitsLegal Abstract These...

  13. NMAC 1.2.2 Public Regulations Commission Rules of Procedure ...

    Open Energy Info (EERE)

    NMAC 1.2.2 Public Regulations Commission Rules of Procedure Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: NMAC 1.2.2...

  14. 1 NMAC 2.2 - Public Regulation Commission Rules of Procedure...

    Open Energy Info (EERE)

    NMAC 2.2 - Public Regulation Commission Rules of Procedure Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 1 NMAC 2.2 -...

  15. Code of Colorado Regulations 2 CCR 601-1, State Highway Access...

    Open Energy Info (EERE)

    Code of Colorado Regulations 2 CCR 601-1, State Highway Access Code Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Code of...

  16. Colorado Code of Regulations 2 CCR 601-18, State Utility Accommodation...

    Open Energy Info (EERE)

    Colorado Code of Regulations 2 CCR 601-18, State Utility Accommodation Code Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  17. WAC 332-17 Geothermal Drilling Rules and Regulations | Open Energy...

    Open Energy Info (EERE)

    17 Geothermal Drilling Rules and Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC 332-17 Geothermal Drilling...

  18. Colorado 4 CCR 723-3001, Definitions for Rules Regulating Electric...

    Open Energy Info (EERE)

    4 CCR 723-3001, Definitions for Rules Regulating Electric Utilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  19. 4 Code of Colorado Regulations (CCR) 723-3 | Open Energy Information

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 4 Code of Colorado Regulations (CCR) 723-3Legal Abstract Colorado Public Utilities...

  20. H.A.R. 13-171 - Designation and Regulation of Water Management...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: H.A.R. 13-171 - Designation and Regulation of Water Management AreasLegal Published NA Year...

  1. Naphthazarin protects against glutamate-induced neuronal death via activation of the Nrf2/ARE pathway

    SciTech Connect (OSTI)

    Son, Tae Gen; Kawamoto, Elisa M.; Yu, Qian-Sheng; Greig, Nigel H.; Mattson, Mark P.; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD ; Camandola, Simonetta

    2013-04-19

    Highlights: Naphthazarin activates the Nrf2/ARE pathway. Naphthazarin induces Nrf2-driven genes in neurons and astrocytes. Naphthazarin protects neurons against excitotoxicity. -- Abstract: Nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway is an important cellular stress response pathway involved in neuroprotection. We previously screened several natural phytochemicals and identified plumbagin as a novel activator of the Nrf2/ARE pathway that can protect neurons against ischemic injury. Here we extended our studies to natural and synthetic derivatives of plumbagin. We found that 5,8-dimethoxy-1,4-naphthoquinone (naphthazarin) is a potent activator of the Nrf2/ARE pathway, up-regulates the expression of Nrf2-driven genes in primary neuronal and glial cultures, and protects neurons against glutamate-induced excitotoxicity.

  2. Inducible secretion of a cellulase from Clostridium thermocellum in Bacillus subtilis

    SciTech Connect (OSTI)

    Joliff, G.; Edelman, A., Klier, A.; Rapoport, G. )

    1989-11-01

    A host-vector system for inducible secretion during the logarithmic growth phase in Bacillus subtilis has been developed. The B. subtilis levansucrase gene promoter and the region encoding its signal sequence have been used. The endoglucanase A of Clostridium thermocellum was used as a model protein to test the efficiency of the system. Effective inducible secretion of the endoglucanase A was observed when either the levansucrase signal sequence or its own signal sequence was used. Expression of the endoglucanase A in different genetic backgrounds of B. subtilis showed that its regulation was similar to that of levansucrase, and high enzyme activity was recovered from the culture supernatant of a hyperproducing B. subtilis sacU(Hy) strain. The molecular weight of 46,000 estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the secreted endoglucanase A is compatible with the calculated molecular weight of the mature polypeptide.

  3. Cell recognition molecule L1 promotes embryonic stem cell differentiation through the regulation of cell surface glycosylation

    SciTech Connect (OSTI)

    Li, Ying; Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian 116023 ; Huang, Xiaohua; Department of Clinical Biochemistry, College of Laboratory Medicine, Dalian Medical University, Dalian 116044 ; An, Yue; Ren, Feng; Yang, Zara Zhuyun; Zhu, Hongmei; Zhou, Lei; Department of Anatomy and Developmental Biology, Monash University, Clayton 3800 ; He, Xiaowen; Schachner, Melitta; Xiao, Zhicheng; Department of Anatomy and Developmental Biology, Monash University, Clayton 3800 ; Ma, Keli; Li, Yali; Department of Anatomy, National University of Singapore, Singapore 119078

    2013-10-25

    Highlights: Down-regulating FUT9 and ST3Gal4 expression blocks L1-induced neuronal differentiation of ESCs. Up-regulating FUT9 and ST3Gal4 expression in L1-ESCs depends on the activation of PLC?. L1 promotes ESCs to differentiate into neuron through regulating cell surface glycosylation. -- Abstract: Cell recognition molecule L1 (CD171) plays an important role in neuronal survival, migration, differentiation, neurite outgrowth, myelination, synaptic plasticity and regeneration after injury. Our previous study has demonstrated that overexpressing L1 enhances cell survival and proliferation of mouse embryonic stem cells (ESCs) through promoting the expression of FUT9 and ST3Gal4, which upregulates cell surface sialylation and fucosylation. In the present study, we examined whether sialylation and fucosylation are involved in ESC differentiation through L1 signaling. RNA interference analysis showed that L1 enhanced differentiation of ESCs into neurons through the upregulation of FUT9 and ST3Gal4. Furthermore, blocking the phospholipase C? (PLC?) signaling pathway with either a specific PLC? inhibitor or knockdown PLC? reduced the expression levels of both FUT9 and ST3Gal4 mRNAs and inhibited L1-mediated neuronal differentiation. These results demonstrate that L1 promotes neuronal differentiation from ESCs through the L1-mediated enhancement of FUT9 and ST3Gal4 expression.

  4. Prediction of epigenetically regulated genes in breast cancer cell lines

    SciTech Connect (OSTI)

    Loss, Leandro A; Sadanandam, Anguraj; Durinck, Steffen; Nautiyal, Shivani; Flaucher, Diane; Carlton, Victoria EH; Moorhead, Martin; Lu, Yontao; Gray, Joe W; Faham, Malek; Spellman, Paul; Parvin, Bahram

    2010-05-04

    Methylation of CpG islands within the DNA promoter regions is one mechanism that leads to aberrant gene expression in cancer. In particular, the abnormal methylation of CpG islands may silence associated genes. Therefore, using high-throughput microarrays to measure CpG island methylation will lead to better understanding of tumor pathobiology and progression, while revealing potentially new biomarkers. We have examined a recently developed high-throughput technology for measuring genome-wide methylation patterns called mTACL. Here, we propose a computational pipeline for integrating gene expression and CpG island methylation profles to identify epigenetically regulated genes for a panel of 45 breast cancer cell lines, which is widely used in the Integrative Cancer Biology Program (ICBP). The pipeline (i) reduces the dimensionality of the methylation data, (ii) associates the reduced methylation data with gene expression data, and (iii) ranks methylation-expression associations according to their epigenetic regulation. Dimensionality reduction is performed in two steps: (i) methylation sites are grouped across the genome to identify regions of interest, and (ii) methylation profles are clustered within each region. Associations between the clustered methylation and the gene expression data sets generate candidate matches within a fxed neighborhood around each gene. Finally, the methylation-expression associations are ranked through a logistic regression, and their significance is quantified through permutation analysis. Our two-step dimensionality reduction compressed 90% of the original data, reducing 137,688 methylation sites to 14,505 clusters. Methylation-expression associations produced 18,312 correspondences, which were used to further analyze epigenetic regulation. Logistic regression was used to identify 58 genes from these correspondences that showed a statistically signifcant negative correlation between methylation profles and gene expression in the panel of breast cancer cell lines. Subnetwork enrichment of these genes has identifed 35 common regulators with 6 or more predicted markers. In addition to identifying epigenetically regulated genes, we show evidence of differentially expressed methylation patterns between the basal and luminal subtypes. Our results indicate that the proposed computational protocol is a viable platform for identifying epigenetically regulated genes. Our protocol has generated a list of predictors including COL1A2, TOP2A, TFF1, and VAV3, genes whose key roles in epigenetic regulation is documented in the literature. Subnetwork enrichment of these predicted markers further suggests that epigenetic regulation of individual genes occurs in a coordinated fashion and through common regulators.

  5. Classical and alternative macrophage activation in the lung following ozone-induced oxidative stress

    SciTech Connect (OSTI)

    Sunil, Vasanthi R.; Patel-Vayas, Kinal; Shen, Jianliang; Laskin, Jeffrey D.; Laskin, Debra L.

    2012-09-01

    Ozone is a pulmonary irritant known to cause oxidative stress, inflammation and tissue injury. Evidence suggests that macrophages play a role in the pathogenic response; however, their contribution depends on the mediators they encounter in the lung which dictate their function. In these studies we analyzed the effects of ozone-induced oxidative stress on the phenotype of alveolar macrophages (AM). Exposure of rats to ozone (2 ppm, 3 h) resulted in increased expression of 8-hydroxy-2?-deoxyguanosine (8-OHdG), as well as heme oxygenase-1 (HO-1) in AM. Whereas 8-OHdG was maximum at 24 h, expression of HO-1 was biphasic increasing after 3 h and 4872 h. Cleaved caspase-9 and beclin-1, markers of apoptosis and autophagy, were also induced in AM 24 h post-ozone. This was associated with increased bronchoalveolar lavage protein and cells, as well as matrix metalloproteinase (MMP)-2 and MMP-9, demonstrating alveolar epithelial injury. Ozone intoxication resulted in biphasic activation of the transcription factor, NF?B. This correlated with expression of monocyte chemotactic protein?1, inducible nitric oxide synthase and cyclooxygenase?2, markers of proinflammatory macrophages. Increases in arginase-1, Ym1 and galectin-3 positive anti-inflammatory/wound repair macrophages were also observed in the lung after ozone inhalation, beginning at 24 h (arginase-1, Ym1), and persisting for 72 h (galectin-3). This was associated with increased expression of pro-surfactant protein-C, a marker of Type II cell proliferation and activation, important steps in wound repair. These data suggest that both proinflammatory/cytotoxic and anti-inflammatory/wound repair macrophages are activated early in the response to ozone-induced oxidative stress and tissue injury. -- Highlights: ? Lung macrophages are highly sensitive to ozone induced oxidative stress. ? Ozone induces autophagy and apoptosis in lung macrophages. ? Proinflammatory and wound repair macrophages are activated early after ozone. ? Oxidative stress may contribute to regulating macrophage phenotype and function.

  6. Schisantherin A suppresses osteoclast formation and wear particle-induced osteolysis via modulating RANKL signaling pathways

    SciTech Connect (OSTI)

    He, Yi; Zhang, Qing; Shen, Yi; Chen, Xia; Zhou, Feng; Peng, Dan

    2014-07-04

    Highlights: • Schisantherin A suppresses osteoclasts formation and function in vitro. • Schisantherin A impairs RANKL signaling pathway. • Schisantherin A suppresses osteolysis in vivo. • Schisantherin A may be used for treating osteoclast related diseases. - Abstract: Receptor activator of NF-κB ligand (RANKL) plays critical role in osteoclastogenesis. Targeting RANKL signaling pathways has been a promising strategy for treating osteoclast related bone diseases such as osteoporosis and aseptic prosthetic loosening. Schisantherin A (SA), a dibenzocyclooctadiene lignan isolated from the fruit of Schisandra sphenanthera, has been used as an antitussive, tonic, and sedative agent, but its effect on osteoclasts has been hitherto unknown. In the present study, SA was found to inhibit RANKL-induced osteoclast formation and bone resorption. The osteoclastic specific marker genes induced by RANKL including c-Src, SA inhibited OSCAR, cathepsin K and TRAP in a dose dependent manner. Further signal transduction studies revealed that SA down-regulate RANKL-induced nuclear factor-kappaB (NF-κB) signaling activation by suppressing the phosphorylation and degradation of IκBα, and subsequently preventing the NF-κB transcriptional activity. Moreover, SA also decreased the RANKL-induced MAPKs signaling pathway, including JNK and ERK1/2 posphorylation while had no obvious effects on p38 activation. Finally, SA suppressed the NF-κB and MAPKs subsequent gene expression of NFATc1 and c-Fos. In vivo studies, SA inhibited osteoclast function and exhibited bone protection effect in wear-particle-induced bone erosion model. Taken together, SA could attenuate osteoclast formation and wear particle-induced osteolysis by mediating RANKL signaling pathways. These data indicated that SA is a promising therapeutic natural compound for the treatment of osteoclast-related prosthesis loosening.

  7. Implications of Carbon Regulation for Green Power Markets

    SciTech Connect (OSTI)

    Bird, Lori; Holt, Ed; Carroll, Ghita

    2007-04-01

    This paper examines the potential effects that emerging mandatory carbon markets have for voluntary markets for renewable energy, or green power markets. In an era of carbon regulation, green power markets will continue to play an important role because many consumers may be interested in supporting renewable energy development beyond what is supported through mandates or other types of policy support. The paper examines the extent to which GHG benefits motivate consumers to make voluntary renewable energy purchases and summarizes key issues emerging as a result of these overlapping markets, such as the implications of carbon regulation for renewable energy marketing claims, the demand for and price of renewable energy certificates (RECs), and the use of RECs in multiple markets (disaggregation of attributes). It describes carbon regulation programs under development in the Northeast and California, and how these might affect renewable energy markets in these regions, as well as the potential interaction between voluntary renewable energy markets and voluntary carbon markets, such as the Chicago Climate Exchange (CCX). It also briefly summarizes the experience in the European Union, where carbon is already regulated. Finally, the paper presents policy options for policymakers and regulators to consider in designing carbon policies to enable carbon markets and voluntary renewable energy markets to work together.

  8. Environmental regulations and changes in petroleum refining operations

    SciTech Connect (OSTI)

    Lidderdale, T.C.M.

    1996-03-01

    The U.S. petroleum industry has responded to four major new federal rules on motor gasoline product quality in the last 6 years: Phase 1 Summer Volatility (Rvp) Regulation, June 1989; Phase 2 Summer Volatility (Rvp) regulation, May 1992; oxygenated Gasoline, November 1992; and reformulated Gasoline Phase I Simple Model, December 1994. These regulations have generated significant changes in domestic refinery operations, affecting marginal production costs and market prices, refinery yields, and the seasonality of production. Some changes have been dramatic. The price of motor gasoline has increased by as much as 60 {cents}/gal due to regulations. Refinery yields of motor gasoline (refinery output of motor gasoline as a fraction of refinery inputs or total refinery output), which historically peaked in the early summer to meet high summer driving demand, now are highest during the winter months. These changes in domestic refining operations are identified and related to the vapor pressure, oxygenated and reformulated gasoline (RFG) product quality regulations. This analysis uses linear regression equations from the Energy Information Administration`s (EIA) Short-Term Integrated Forecasting System (STIFS). The STIFS model is used for producing forecasts appearing in the EIA`s Short-Term Energy Outlook.

  9. Air-flow regulation system for a coal gasifier

    DOE Patents [OSTI]

    Fasching, George E.

    1984-01-01

    An improved air-flow regulator for a fixed-bed coal gasifier is provided which allows close air-flow regulation from a compressor source even though the pressure variations are too rapid for a single primary control loop to respond. The improved system includes a primary controller to control a valve in the main (large) air supply line to regulate large slow changes in flow. A secondary controller is used to control a smaller, faster acting valve in a secondary (small) air supply line parallel to the main line valve to regulate rapid cyclic deviations in air flow. A low-pass filter with a time constant of from 20 to 50 seconds couples the output of the secondary controller to the input of the primary controller so that the primary controller only responds to slow changes in the air-flow rate, the faster, cyclic deviations in flow rate sensed and corrected by the secondary controller loop do not reach the primary controller due to the high frequency rejection provided by the filter. This control arrangement provides at least a factor of 5 improvement in air-flow regulation for a coal gasifier in which air is supplied by a reciprocating compressor through a surge tank.

  10. Polymeric black tea polyphenols inhibit 1,2-dimethylhydrazine induced colorectal carcinogenesis by inhibiting cell proliferation via Wnt/{beta}-catenin pathway

    SciTech Connect (OSTI)

    Patel, Rachana; Ingle, Arvind; Maru, Girish B.

    2008-02-15

    Tea polyphenols like epigallocatechin gallate and theaflavins are established chemopreventive agents for colorectal carcinogenesis. However, studies on evaluating similar chemopreventive properties of thearubigins or polymeric black tea polyphenols (PBPs), the most abundant polyphenols in black tea, are limited. Hence, in the present study we aim to investigate chemopreventive effects along with probable mechanisms of action of PBP extract employing 1,2-dimethylhydrazine (DMH)-induced colorectal carcinogenesis in Sprague-Dawley rats as experimental model. The present study suggests that PBPs, like other tea polyphenols, also inhibit DMH-induced colorectal tumorigenesis by decreasing tumor volume and multiplicity. This study also shows that although the pretreatment with PBP extract could induce detoxifying enzymes in hepatic and colorectal tissue, it did not show any additional chemopreventive effects when compared to treatments with PBP extract after initiation with DMH. Mechanistically, PBP extract may inhibit colorectal carcinogenesis by decreasing DMH-induced cell proliferation via Wnt/{beta}-catenin pathway. Treatments with PBP extract showed decreased levels of COX-2, c-MYC and cyclin D1 proteins which aid cell proliferation probably by regulating {beta}-catenin by maintaining expression of APC and decreasing inactivation of GSK3{beta}. DMH-induced activation of MAP kinases such as ERK and JNK was also found to be inhibited by treatments with PBP extract. In conclusion, the protective effects of PBP extract could be attributed to inhibition of DMH-induced cellular proliferation probably through {beta}-catenin regulation.

  11. Norathyriol Suppresses Skin Cancers Induced by Solar Ultraviolet Radiation by Targeting ERK Kinases

    SciTech Connect (OSTI)

    Li, Jixia; Malakhova, Margarita; Mottamal, Madhusoodanan; Reddy, Kanamata; Kurinov, Igor; Carper, Andria; Langfald, Alyssa; Oi, Naomi; Kim, Myoung Ok; Zhu, Feng; Sosa, Carlos P.; Zhou, Keyuan; Bode, Ann M.; Dong, Zigang

    2012-06-27

    Ultraviolet (UV) irradiation is the leading factor in the development of skin cancer, prompting great interest in chemopreventive agents for this disease. In this study, we report the discovery of norathyriol, a plant-derived chemopreventive compound identified through an in silico virtual screening of the Chinese Medicine Library. Norathyriol is a metabolite of mangiferin found in mango, Hypericum elegans, and Tripterospermum lanceolatum and is known to have anticancer activity. Mechanistic investigations determined that norathyriol acted as an inhibitor of extracellular signal-regulated kinase (ERK)1/2 activity to attenuate UVB-induced phosphorylation in mitogen-activated protein kinases signaling cascades. We confirmed the direct and specific binding of norathyriol with ERK2 through a cocrystal structural analysis. The xanthone moiety in norathyriol acted as an adenine mimetic to anchor the compound by hydrogen bonds to the hinge region of the protein ATP-binding site on ERK2. Norathyriol inhibited in vitro cell growth in mouse skin epidermal JB6 P+ cells at the level of G{sub 2}-M phase arrest. In mouse skin tumorigenesis assays, norathyriol significantly suppressed solar UV-induced skin carcinogenesis. Further analysis indicated that norathyriol mediates its chemopreventive activity by inhibiting the ERK-dependent activity of transcriptional factors AP-1 and NF-{kappa}B during UV-induced skin carcinogenesis. Taken together, our results identify norathyriol as a safe new chemopreventive agent that is highly effective against development of UV-induced skin cancer.

  12. Sirt2 suppresses inflammatory responses in collagen-induced arthritis

    SciTech Connect (OSTI)

    Lin, Jiangtao; Department of Orthopaedics, Yantaishan Hospital, 91 Jiefang Road, Yantai, Shandong 264001 ; Sun, Bing; Jiang, Chuanqiang; Hong, Huanyu; Zheng, Yanping

    2013-11-29

    Highlights: Sirt2 expression decreases in collagen-induced arthritis (CIA). Sirt2 knockout aggravates severity of arthritis in mice with CIA. Sirt2 knockout increases levels of pro-inflammatory factors in the serum. Sirt2 deacetylates p65 and inhibits pro-inflammatory factors expression. Sirt2 rescue abates severity of arthritis in mice with CIA. -- Abstract: Arthritis is a common autoimmune disease that is associated with progressive disability, systemic complications and early death. However, the underling mechanisms of arthritis are still unclear. Sirtuins are a NAD{sup +}-dependent class III deacetylase family, and regulate cellular stress, inflammation, genomic stability, carcinogenesis, and energy metabolism. Among the sirtuin family members, Sirt1 and Sirt6 are critically involved in the development of arthritis. It remains unknown whether other sirtuin family members participate in arthritis. Here in this study, we demonstrate that Sirt2 inhibits collagen-induced arthritis (CIA) using in vivo and in vitro evidence. The protein and mRNA levels of Sirt2 significantly decreased in joint tissues of mice with CIA. When immunized with collagen, Sirt2-KO mice showed aggravated severity of arthritis based on clinical scores, hind paw thickness, and radiological and molecular findings. Mechanically, Sirt2 deacetylated p65 subunit of nuclear factor-kappa B (NF-?B) at lysine 310, resulting in reduced expression of NF-?B-dependent genes, including interleukin 1? (IL-1?), IL-6, monocyte chemoattractant protein 1(MCP-1), RANTES, matrix metalloproteinase 9 (MMP-9) and MMP-13. Importantly, our rescue experiment showed that Sirt2 re-expression abated the severity of arthritis in Sirt2-KO mice. Those findings strongly indicate Sirt2 as a considerably inhibitor of the development of arthritis.

  13. Ion-acoustic shocks with self-regulated ion reflection and acceleratio...

    Office of Scientific and Technical Information (OSTI)

    Ion-acoustic shocks with self-regulated ion reflection and acceleration Citation Details ... Title: Ion-acoustic shocks with self-regulated ion reflection and acceleration Authors: ...

  14. Montana Rule 36.25.4 Geothermal Rules and Regulations | Open...

    Open Energy Info (EERE)

    Geothermal Rules and RegulationsLegal Abstract Montana regulation governing administration of geothermal resources in the state. Published NA Year Signed or Took Effect...

  15. Mangiferin exerts antitumor activity in breast cancer cells by regulating matrix metalloproteinases, epithelial to mesenchymal transition, and ?-catenin signaling pathway

    SciTech Connect (OSTI)

    Li, Hongzhong; Huang, Jing; Yang, Bing; Xiang, Tingxiu; Yin, Xuedong; Peng, Weiyan; Cheng, Wei; Wan, Jingyuan; Luo, Fuling; Li, Hongyuan; Ren, Guosheng

    2013-10-01

    Although mangiferin which is a naturally occurring glucosylxanthone has exhibited promising anticancer activities, the detailed molecular mechanism of mangiferin on cancers still remains enigmatic. In this study, the anticancer activity of mangiferin was evaluated in breast cancer cell line-based in vitro and in vivo models. We showed that mangiferin treatment resulted in decreased cell viability and suppression of metastatic potential in breast cancer cells. Further mechanistic investigation revealed that mangiferin induced decreased matrix metalloproteinase (MMP)-7 and -9, and reversal of epithelialmesenchymal transition (EMT). Moreover, it was demonstrated that mangiferin significantly inhibited the activation of ?-catenin pathway. Subsequent experiments showed that inhibiting ?-catenin pathway might play a central role in mangiferin-induced anticancer activity through modulation of MMP-7 and -9, and EMT. Consistent with these findings in vitro, the antitumor potential was also verified in mangiferin-treated MDA-MB-231 xenograft mice where significantly decreased tumor volume, weight and proliferation, and increased apoptosis were obtained, with lower expression of MMP-7 and -9, vimentin and active ?-catenin, and higher expression of E-cadherin. Taken together, our study suggests that mangiferin might be used as an effective chemopreventive agent against breast cancer. - Highlights: Mangiferin inhibits growth and metastatic potential in breast cancer cells. Mangiferin down-regulates MMP-7 and -9 in breast cancer cells. Mangiferin induces the reversal of EMT in metastatic breast cancer cells. Mangiferin inhibits the activation of ?-catenin pathway in breast cancer cells. Inhibiting ?-catenin is responsible for the antitumor activity of mangiferin.

  16. Feedback regulated induction heater for a flowing fluid

    DOE Patents [OSTI]

    Migliori, A.; Swift, G.W.

    1984-06-13

    A regulated induction heater for heating a stream of flowing fluid to a predetermined desired temperature. The heater includes a radiofrequency induction coil which surrounds a glass tube through which the fluid flows. A heating element consisting of a bundle of approximately 200 stainless steel capillary tubes located within the glass tube couples the output of the induction coil to the fluid. The temperature of the fluid downstream from the heating element is sensed with a platinum resistance thermometer, the output of which is applied to an adjustable porportional and integral feedback control circuit which regulates the power applied to the induction coil. The heater regulates the fluid temperature to within 0.005/sup 0/C at a flow rate of 50 cm/sup 3//sec with a response time of less than 0.1 second, and can accommodate changes in heat load up to 1500 watts.

  17. Feedback regulated induction heater for a flowing fluid

    DOE Patents [OSTI]

    Migliori, Albert; Swift, Gregory W.

    1985-01-01

    A regulated induction heater for heating a stream of flowing fluid to a predetermined desired temperature. The heater includes a radiofrequency induction coil which surrounds a glass tube through which the fluid flows. A heating element consisting of a bundle of approximately 200 stainless steel capillary tubes located within the glass tube couples the output of the induction coil to the fluid. The temperature of the fluid downstream from the heating element is sensed with a platinum resistance thermometer, the output of which is applied to an adjustable proportional and integral feedback control circuit which regulates the power applied to the induction coil. The heater regulates the fluid temperature to within 0.005.degree. C. at a flow rate of 50 cm.sup.3 /second with a response time of less than 0.1 second, and can accommodate changes in heat load up to 1500 watts.

  18. Interface Induced Carbonate Mineralization: A Fundamental Geochemical

    Office of Scientific and Technical Information (OSTI)

    Process Relevant to Carbon Sequestration (Technical Report) | SciTech Connect Interface Induced Carbonate Mineralization: A Fundamental Geochemical Process Relevant to Carbon Sequestration Citation Details In-Document Search Title: Interface Induced Carbonate Mineralization: A Fundamental Geochemical Process Relevant to Carbon Sequestration We have approached the long-standing geochemical question why anhydrous high-Mg carbonate minerals (i.e., magnesite and dolomite) cannot be formed at

  19. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Disorder-Induced Microscopic Magnetic Memory Print Wednesday, 26 October 2005 00:00 The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired magnetic properties. But as the density of magnetic disks climbs, the size of the magnetic domains responsible for storage must decrease, posing new challenges. Beautiful theories based on random microscopic disorder have been developed over

  20. Atmospheric Radiation Measurement Convective and Orographically Induced

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Convective and Orographically Induced Precipitation Study The U.S. Department of Energy's Atmospheric Radiation Measurement (ARM) Climate Research Facility is providing the ARM Mobile Facility (AMF) to support a long-term precipitation study in the Black Forest region of Germany. Requested by researchers from the University of Hohenheim, the AMF will be deployed as one of four heav- ily instrumented supersites established for the Convective and Orographically Induced Precipita- tion Study

  1. MTBreg: The Database of Conditionally Regulated Proteins in Mycobacterium Tuberculosis

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kaufman, Markus; Pal, Debnath; Eisenberg, David

    Proteins up- and down- regulated in Mycobacterium tuberculosis grown under conditions mimicking infection are included in this database. It also includes information on proteins that are regulated by selected transcription factors or other regulatory proteins. The literature data provided here is complimentary to the databases provided by Michael Strong that include recent TB computational functional linkages and the Prolinks Database by Peter Bowers. The experimental condition, the experimental dataset and a literature reference will be displayed, including links to the computationally linked proteins in the Prolinks Database and the entry in the Mycobacterium tuberculosis Structural Genomics Database.[Copied from information at http://www.doe-mbi.ucla.edu/Services/MTBreg/

  2. Will regulation impede the market for emissions allowances

    SciTech Connect (OSTI)

    Anderson, B.S.; Casey, W.T.

    1994-03-15

    Title IV of the Clean Air Act Amendments of 1990 allow for trading of allowances for SO[sub 2] emissions. For this process to be efficient and to achieve the goal of reducing emissions by 10 million tons from 1980 levels, a freely functioning market in allowance trading needs to exist. This market could be threatened by some state regulations that require utilities to obtain prior approval from state commissions before selling property. There are several solutions for state regulators to employ to avoid inadvertently interfering with the allowance trading market. This article describes some of those possible solutions.

  3. Emission Regulations Reduced Impact of Climate Change in CA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Emission Regulations Reduced Impact of Climate Change in CA Emission Regulations Reduced Impact of Climate Change in CA Study shows clean diesel programs slashed black carbon, a powerful short-term contributor to global warming June 13, 2013 Jon Weiner 510-486-4014 jrweiner@lbl.gov CA-BC-graphic.jpg Sacramento - Reductions in emissions of black carbon since the late 1980s, mostly from diesel engines as a result of air quality programs, have resulted in a measurable reduction of concentrations of

  4. DOE - Office of Legacy Management -- Minneapolis-Honeywell Regulator Co -

    Office of Legacy Management (LM)

    PA 30 Minneapolis-Honeywell Regulator Co - PA 30 FUSRAP Considered Sites Site: MINNEAPOLIS-HONEYWELL REGULATOR CO (PA.30 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: Brown Instrument Division PA.30-1 Location: Wayne and Roberts Avenue , Philadelphia , Pennsylvania PA.30-2 Evaluation Year: 1987 PA.30-3 Site Operations: Research/Development and Testing - small-scale thermal conductivity test on uranium rods - early 1950s. PA.30-1 PA.30-2

  5. Multi-Pollutant Legislation and Regulations (released in AEO2005)

    Reports and Publications (EIA)

    2005-01-01

    The 108th Congress proposed and debated a variety of bills addressing pollution control at electric power plants but did not pass any of them into law. In addition, the Environmental Protection Agency (EPA) currently is preparing two regulations-a proposed Clean Air Interstate Rule (pCAIR) and a Clean Air Mercury Rule (CAMR)-to address emissions from coal-fired power plants. Several states also have taken legislative actions to limit pollutants from power plants in their jurisdictions. This section discusses three Congressional air pollution bills and the EPA's pCAIR and CAMR regulations.

  6. Protocol for Addressing Induced Seismicity Associated with Enhanced...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems (EGS) Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems...

  7. Spectral changes in layered f -electron systems induced by Kondo...

    Office of Scientific and Technical Information (OSTI)

    Spectral changes in layered f -electron systems induced by Kondo hole substitution in the boundary layer Title: Spectral changes in layered f -electron systems induced by Kondo ...

  8. Magnus-induced ratchet effects for skyrmions interacting with...

    Office of Scientific and Technical Information (OSTI)

    Published Article: Magnus-induced ratchet effects for skyrmions interacting with asymmetric substrates Title: Magnus-induced ratchet effects for skyrmions interacting with ...

  9. Magnus-induced ratchet effects for skyrmions interacting with...

    Office of Scientific and Technical Information (OSTI)

    Magnus-induced ratchet effects for skyrmions interacting with asymmetric substrates Citation Details In-Document Search Title: Magnus-induced ratchet effects for skyrmions ...

  10. Radiation-induced mechanical property changes in filled rubber...

    Office of Scientific and Technical Information (OSTI)

    Radiation-induced mechanical property changes in filled rubber Citation Details In-Document Search Title: Radiation-induced mechanical property changes in filled rubber You are ...

  11. Radiation-induced mechanical property changes in filled rubber...

    Office of Scientific and Technical Information (OSTI)

    Radiation-induced mechanical property changes in filled rubber Citation Details In-Document Search Title: Radiation-induced mechanical property changes in filled rubber Authors: ...

  12. Behavior of tritium permeation induced by water corrosion of...

    Office of Scientific and Technical Information (OSTI)

    induced by water corrosion of alpha iron around room temperature Citation Details In-Document Search Title: Behavior of tritium permeation induced by water corrosion of ...

  13. Bending-induced Symmetry Breaking of Lithiation in Germanium...

    Office of Scientific and Technical Information (OSTI)

    Bending-induced Symmetry Breaking of Lithiation in Germanium Nanowires Citation Details In-Document Search Title: Bending-induced Symmetry Breaking of Lithiation in Germanium ...

  14. Sustainability of Shear-Induced Permeability for EGS Reservoirs...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sustainability of Shear-Induced Permeability for EGS Reservoirs A Laboratory Study Sustainability of Shear-Induced Permeability for EGS Reservoirs A Laboratory Study ...

  15. Potential Induced Degradation (PID) Tests for Commercially Available...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Potential Induced Degradation (PID) Tests for Commercially Available PV Modules Potential Induced Degradation (PID) Tests for Commercially Available PV Modules Presented at the PV ...

  16. " A Heterodyne Laser-induced Fluorescence Technique to Determine...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Heterodyne Laser-induced Fluorescence Technique to Determine Simultaneously the Bulk ... molecule velocity distribution using a heterodyne laser induced fluorescence technique. ...

  17. Detection and Characterization of Natural and Induced Fractures...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Detection and Characterization of Natural and Induced Fractures for the Development of Enhanced Geothermal Systems Detection and Characterization of Natural and Induced Fractures for ...

  18. Sandia Energy - BES Highlight: Stress-Induced NanoparticleCrystalliza...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Highlight: Stress-Induced Nanoparticle Crystallization Home Highlights - Energy Research BES Highlight: Stress-Induced Nanoparticle Crystallization Previous Next BES Highlight:...

  19. Description of Induced Nuclear Fission with Skyrme Energy Functionals...

    Office of Scientific and Technical Information (OSTI)

    of Induced Nuclear Fission with Skyrme Energy Functionals: II. Finite Temperature Effects Citation Details In-Document Search Title: Description of Induced Nuclear Fission with ...

  20. Proximity-induced magnetism in transition-metal substituted graphene...

    Office of Scientific and Technical Information (OSTI)

    Proximity-induced magnetism in transition-metal substituted graphene Title: Proximity-induced magnetism in transition-metal substituted graphene We investigate the interactions ...

  1. Fault-induced delayed voltage recovery in a long inhomogeneous...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Fault-induced delayed voltage recovery in a long inhomogeneous power-distribution feeder Citation Details In-Document Search Title: Fault-induced delayed voltage ...

  2. Direct optical detection of current induced spin accumulation...

    Office of Scientific and Technical Information (OSTI)

    harmonic generation Citation Details In-Document Search Title: Direct optical detection of current induced spin accumulation in metals by magnetization-induced second harmonic ...

  3. Pressure-Induced Amorphization and Phase Transformations in...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Pressure-Induced Amorphization and Phase Transformations in beta-LiAlSiOsubscript 4 Citation Details In-Document Search Title: Pressure-Induced Amorphization ...

  4. Description of induced nuclear fission with Skyrme energy functionals...

    Office of Scientific and Technical Information (OSTI)

    Description of induced nuclear fission with Skyrme energy functionals: Static potential ... Citation Details In-Document Search Title: Description of induced nuclear fission with ...

  5. Description of Induced Nuclear Fission with Skyrme Energy Functionals...

    Office of Scientific and Technical Information (OSTI)

    Description of Induced Nuclear Fission with Skyrme Energy Functionals: II. Finite Temperature Effects Citation Details In-Document Search Title: Description of Induced Nuclear ...

  6. Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice

    SciTech Connect (OSTI)

    Kawai, Ikuma; Hisaki, Tomoka; Sugiura, Koji; Naito, Kunihiko; Kano, Kiyoshi; Biomedical Science Center for Translational Research , The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515

    2012-10-26

    Highlights: Black-Right-Pointing-Pointer Discoidin domain receptor 2 (DDR2) is a receptor tyrosine kinase. Black-Right-Pointing-Pointer DDR2 regulates cell proliferation, cell adhesion, migration, and extracellular matrix remodeling. Black-Right-Pointing-Pointer We produced in vitro and in vivo model to better understand the role of DDR2. Black-Right-Pointing-Pointer DDR2 might play an inhibitory role in the proliferation of chondrocyte. -- Abstract: Discoidin domain receptor 2 (DDR2) is a receptor tyrosine kinase that is activated by fibrillar collagens. DDR2 regulates cell proliferation, cell adhesion, migration, and extracellular matrix remodeling. The decrement of endogenous DDR2 represses osteoblastic marker gene expression and osteogenic differentiation in murine preosteoblastic cells, but the functions of DDR2 in chondrogenic cellular proliferation remain unclear. To better understand the role of DDR2 signaling in cellular proliferation in endochondral ossification, we inhibited Ddr2 expression via the inhibitory effect of miRNA on Ddr2 mRNA (miDdr2) and analyzed the cellular proliferation and differentiation in the prechondrocyte ATDC5 cell lines. To investigate DDR2's molecular role in endochondral cellular proliferation in vivo, we also produced transgenic mice in which the expression of truncated, kinase dead (KD) DDR2 protein is induced, and evaluated the DDR2 function in cellular proliferation in chondrocytes. Although the miDdr2-transfected ATDC5 cell lines retained normal differentiation ability, DDR2 reduction finally promoted cellular proliferation in proportion to the decreasing ratio of Ddr2 expression, and it also promoted earlier differentiation to cartilage cells by insulin induction. The layer of hypertrophic chondrocytes in KD Ddr2 transgenic mice was not significantly thicker than that of normal littermates, but the layer of proliferative chondrocytes in KD-Ddr2 transgenic mice was significantly thicker than that of normal littermates. Taken together, our data demonstrated that DDR2 might play a local and essential role in the proliferation of chondrocytes.

  7. Harmonization - Two Years' of Transportation Regulation Lessons Learned

    SciTech Connect (OSTI)

    Colborn, K.

    2007-07-01

    The U.S. Department of Transportation issued modifications to the Hazardous Materials Regulations in October, 2004 as part of an ongoing effort to 'harmonize' U.S. regulations with those of the International Atomic Energy Agency. The harmonization effort had several predictable effects on low level radioactive materials shipment that were anticipated even prior to their implementation. However, after two years' experience with the new regulations, transporters have identified several effects on transportation which were not entirely apparent when the regulations were first implemented. This paper presents several case studies in the transportation of low level radioactive materials since the harmonization rules took effect. In each case, an analysis of the challenge posed by the regulatory revision is provided. In some cases, more than one strategy for compliance was considered, and the advantages and disadvantages of each are discussed. In several cases, regulatory interpretations were sought and obtained, and these are presented to clarify the legitimacy of the compliance approach. The presentation of interpretations will be accompanied by reports of clarifying discussions with the U.S. DOT about the interpretation and scope of the regulatory change. Specific transportation issues raised by the revised hazardous materials regulations are reviewed, including: The new definition of radioactive material in accordance with isotope-specific concentration and total activity limits. The new hazardous materials regulations (HMR) created a new definition for radioactive material. A case study is presented for soils contaminated with low levels of Th-230. These soils had been being shipped for years as exempt material under the old 2,000 pCi/g concentration limit. Under the new HMR, these same soils were radioactive material. Further, in rail-car quantities their activity exceeded an A2 value, so shipment of the material in gondolas appeared to require an IP-2 package. Interpretations, discussions, and an exemption were obtained to secure the continued shipment of this material. A provision to allow 'natural' radioactive materials to be exempt from the requirements of the HMR at up to 10x the listed isotopic concentrations. The revised HMR exempts certain natural materials and ores from regulation as radioactive material at concentrations up to 10x that allowed if the materials are not natural. The term 'natural' is not well defined, and initial attempts to qualify for this exemption were thwarted by concerns over what degree of material processing, if any, materials could experience and still be considered 'natural'. The presentation includes an example from a project involving post-processed tungsten ore, and includes interpretations from the US DOT as well as clarifying language from current and drafted IAEA regulation and guidance. New packaging descriptions allowing the use of cargo containers as IP-2 and IP-3 packages in some applications. The revised HMR provides an alternate certification procedure under which standard cargo containers can be used as IP-2 and IP-3 containers. There has been some confusion about how this high level of certification can apply to standard cargo containers when other sections of the regulations make this certification available only to considerably more stout containers after rigorous testing. The discussion includes interpretive guidance from the US DOT, and from the UK Department of Transport clarifying the same provision in IAEA regulations. A new definition of contamination with apparently broad impact on the shipment of empty containers and conveyances. The revised HMR presented a definition of contamination not referenced by any other part of the HMR. The preamble to the revised HMR provides confusing guidance on the application of the definition to shipment of empty containers, and subsequent interpretive guidance letters appear to conflict with the preamble as well as with each other. The definition also has the effect of regulating materials for transport as radioactive even when US

  8. Insulin like growth factor 2 regulation of aryl hydrocarbon receptor in MCF-7 breast cancer cells

    SciTech Connect (OSTI)

    Tomblin, Justin K.; Salisbury, Travis B.

    2014-01-17

    Highlights: IGF-2 stimulates concurrent increases in AHR and CCND1 expression. IGF-2 promotes the binding of AHR to the endogenous cyclin D1 promoter. AHR knockdown inhibits IGF-2 stimulated increases in CCND1 mRNA and protein. AHR knockdown inhibits IGF-2 stimulated increases in MCF-7 proliferation. -- Abstract: Insulin like growth factor (IGF)-1 and IGF-2 stimulate normal growth, development and breast cancer cell proliferation. Cyclin D1 (CCND1) promotes cell cycle by inhibiting retinoblastoma protein (RB1). The aryl hydrocarbon receptor (AHR) is a major xenobiotic receptor that also regulates cell cycle. The purpose of this study was to investigate whether IGF-2 promotes MCF-7 breast cancer proliferation by inducing AHR. Western blot and quantitative real time PCR (Q-PCR) analysis revealed that IGF-2 induced an approximately 2-fold increase (P < .001) in the expression of AHR and CCND1. Chromatin immunoprecipitation (ChIP), followed by Q-PCR indicated that IGF-2 promoted (P < .001) a 7-fold increase in AHR binding on the CCND1 promoter. AHR knockdown significantly (P < .001) inhibited IGF-2 stimulated increases in CCND1 mRNA and protein. AHR knockdown cells were less (P < .001) responsive to the proliferative effects of IGF-2 than control cells. Collectively, our findings have revealed a new regulatory mechanism by which IGF-2 induction of AHR promotes the expression of CCND1 and the proliferation of MCF-7 cells. This previously uncharacterized pathway could be important for the proliferation of IGF responsive cancer cells that also express AHR.

  9. Inhibition of IRAK-4 activity for rescuing endotoxin LPS-induced septic mortality in mice by lonicerae flos extract

    SciTech Connect (OSTI)

    Park, Sun Hong; Roh, Eunmiri; Kim, Hyun Soo; Baek, Seung-Il; Choi, Nam Song; Kim, Narae; Hwang, Bang Yeon; Han, Sang-Bae; Kim, Youngsoo

    2013-12-13

    Highlights: •Lonicerae flos extract (HS-23) is a clinical candidate, Phase I for sepsis treatment. •Here, HS-23 or its major constituents rescued LPS-induced septic mortality in mice. •As a mechanism, they directly inhibited IRAK-4-catalyzed kinase activity. •Thus, they suppressed LPS-induced expression of NF-κB/AP-1-target inflammatory genes. -- Abstract: Lonicerae flos extract (HS-23) is a clinical candidate currently undergoing Phase I trial in lipopolysaccharide (LPS)-injected healthy human volunteers, but its molecular basis remains to be defined. Here, we investigated protective effects of HS-23 or its major constituents on Escherichia coli LPS-induced septic mortality in mice. Intravenous treatment with HS-23 rescued LPS-intoxicated C57BL/6J mice under septic conditions, and decreased the levels of cytokines such as tumor necrosis factor α (TNF-α), interleukin (IL)-1β and high-mobility group box-1 (HMGB-1) in the blood. Chlorogenic acid (CGA) and its isomers were assigned as major constituents of HS-23 in the protection against endotoxemia. As a molecular mechanism, HS-23 or CGA isomers inhibited endotoxin LPS-induced autophosphorylation of the IL-1 receptor-associated kinase 4 (IRAK-4) in mouse peritoneal macrophages as well as the kinase activity of IRAK-4 in cell-free reactions. HS-23 consequently suppressed downstream pathways critical for LPS-induced activation of nuclear factor (NF)-κB or activating protein 1 (AP-1) in the peritoneal macrophages. HS-23 also inhibited various toll-like receptor agonists-induced nitric oxide (NO) production, and down-regulated LPS-induced expression of NF-κB/AP-1-target inflammatory genes in the cells. Taken together, HS-23 or CGA isomers exhibited anti-inflammatory therapy against LPS-induced septic mortality in mice, at least in part, mediated through the inhibition of IRAK-4.

  10. Radiation-Induced Notch Signaling in Breast Cancer Stem Cells

    SciTech Connect (OSTI)

    Lagadec, Chann; Vlashi, Erina; Alhiyari, Yazeed; Phillips, Tiffany M.; Bochkur Dratver, Milana; Pajonk, Frank

    2013-11-01

    Purpose: To explore patterns of Notch receptor and ligand expression in response to radiation that could be crucial in defining optimal dosing schemes for ?-secretase inhibitors if combined with radiation. Methods and Materials: Using MCF-7 and T47D breast cancer cell lines, we used real-time reverse transcriptionpolymerase chain reaction to study the Notch pathway in response to radiation. Results: We show that Notch receptor and ligand expression during the first 48 hours after irradiation followed a complex radiation dosedependent pattern and was most pronounced in mammospheres, enriched for breast cancer stem cells. Additionally, radiation activated the Notch pathway. Treatment with a ?-secretase inhibitor prevented radiation-induced Notch family gene expression and led to a significant reduction in the size of the breast cancer stem cell pool. Conclusions: Our results indicate that, if combined with radiation, ?-secretase inhibitors may prevent up-regulation of Notch receptor and ligand family members and thus reduce the number of surviving breast cancer stem cells.

  11. Differences in the regulation of chemicals and radionuclides

    SciTech Connect (OSTI)

    Travis, C.C.

    1993-12-01

    Government regulations limiting public exposure to radionuclides and chemicals have historically been developed by regulatory agencies using different approaches with the result that levels of protection vary for the two classes of contaminants. These differences create difficulties in determining equitable regulatory measures when both radionuclides and chemical pollutants are involved. Generally, radiation exposure is not regulated as stringently as chemical exposure (Travis et al, 1989). The International commission on Radiological Protection (ICRP) recommends limiting excess environmental radiation exposure to the general public to 100 millirem per year (mrem/yr) (ICRP, 1991), a lifetime cancer risk of about 3.5E-3. An acceptable level of risk for chemical exposures is generally considered to be below 1E-6. Differences in regulatory approach for radionuclides and chemicals evoke debate over why they are different and which regulation strategy is better. Because these pollutants often coexist (mixed waste sites, contaminated metals and facilities, etc.), it is important to analyze inconsistencies in the regulation of chemicals and radionuclides and establish a more consistent approach to defining an acceptable level of exposure for these contaminants.

  12. Crafting regulations in emerging geothermal countries: The Peru example

    SciTech Connect (OSTI)

    Armstrong, A.J.

    1996-12-31

    Conventional wisdom holds that no prudent investor or lender will ante up a penny of investment money in a geothermal project unless and until there is a geothermal resources law in place. Since every law depends on a regulatory regime to make the law work in actual practice, implemented regulations are equally important conditions precedent for geothermal development. In recognition of the importance of assisting geothermal regulatory development in the emerging geothermal countries of Latin America, during the 1995 to 1996 timeframe, the Geothermal Energy Association has acted in an advisory capacity to the Ministry of Energy and Mines of Peru, in the drafting of geothermal regulations for Peru. These regulations are designed to promote developmental investment in the geothermal resources of Peru, while simultaneously establishing reasonable standards for the protection of the people and the environment of the country. While these regulations are specific to Peru, they may well serve as a model for other countries of Latin America. Thus, the lessons learned in crafting the Peru regulatory regime may have applicability in other countries in which the geothermal industry is now working or may work in the future.

  13. Cell fate regulation governed by a repurposed bacterial histidine kinase

    SciTech Connect (OSTI)

    Childers, W. Seth; Xu, Qingping; Mann, Thomas H.; Mathews, Irimpan I.; Blair, Jimmy A.; Deacon, Ashley M.; Shapiro, Lucy; Stock, Ann M.

    2014-10-28

    One of the simplest organisms to divide asymmetrically is the bacterium Caulobacter crescentus. The DivL pseudo-histidine kinase, positioned at one cell pole, regulates cell-fate by controlling the activation of the global transcription factor CtrA via an interaction with the response regulator (RR) DivK. DivL uniquely contains a tyrosine at the histidine phosphorylation site, and can achieve these regulatory functions in vivo without kinase activity. Determination of the DivL crystal structure and biochemical analysis of wild-type and site-specific DivL mutants revealed that the DivL PAS domains regulate binding specificity for DivK~P over DivK, which is modulated by an allosteric intramolecular interaction between adjacent domains. We discovered that DivL's catalytic domains have been repurposed as a phosphospecific RR input sensor, thereby reversing the flow of information observed in conventional histidine kinase (HK)-RR systems and coupling a complex network of signaling proteins for cell-fate regulation.

  14. NUCLEAR REGULATOR%'-C~fiMls6N REGION I

    Office of Legacy Management (LM)

    jNrTED ST&ye I ) r P, NUCLEAR REGULATOR%'-CfiMls6N REGION I 63I PIIRK AVENUE Wcs:in;kcse Elecric Coroorrzion ATTiN: 2. F:. jhaw I-- Zivision Piant Manaaer wm.1.1 I SJeszi ...

  15. OSHA safety regulation calls for step-by-step approach

    SciTech Connect (OSTI)

    Bellomo, P.J. (Arthur D. Little Inc., Houston, TX (US))

    1992-06-01

    The U.S. Occupational Safety and Health Administration's long-awaited process safety management (PSM) regulation mandates the implementation of a PSM program at facilities handling highly hazardous materials, including oil refineries and petrochemical plants. This article presents a step-by-step PSM program compliance strategy, delineated and explored through practical examples.

  16. State Regulators Promote Consumer Choice in Retail Gas Markets

    Reports and Publications (EIA)

    1996-01-01

    Restructuring of interstate pipeline companies has created new choices and challenges for local distribution companies (LDCs), their regulators, and their customers. The process of separating interstate pipeline gas sales from transportation service has been completed and has resulted in greater gas procurement options for LDCs.

  17. Cell fate regulation governed by a repurposed bacterial histidine kinase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Childers, W. Seth; Xu, Qingping; Mann, Thomas H.; Mathews, Irimpan I.; Blair, Jimmy A.; Deacon, Ashley M.; Shapiro, Lucy; Stock, Ann M.

    2014-10-28

    One of the simplest organisms to divide asymmetrically is the bacterium Caulobacter crescentus. The DivL pseudo-histidine kinase, positioned at one cell pole, regulates cell-fate by controlling the activation of the global transcription factor CtrA via an interaction with the response regulator (RR) DivK. DivL uniquely contains a tyrosine at the histidine phosphorylation site, and can achieve these regulatory functions in vivo without kinase activity. Determination of the DivL crystal structure and biochemical analysis of wild-type and site-specific DivL mutants revealed that the DivL PAS domains regulate binding specificity for DivK~P over DivK, which is modulated by an allosteric intramolecular interactionmore » between adjacent domains. We discovered that DivL's catalytic domains have been repurposed as a phosphospecific RR input sensor, thereby reversing the flow of information observed in conventional histidine kinase (HK)-RR systems and coupling a complex network of signaling proteins for cell-fate regulation.« less

  18. Mandatory petroleum price and allocation regulations: a history and analysis

    SciTech Connect (OSTI)

    Lane, W.C. Jr.

    1981-05-05

    This study is a history and analysis of Federal controls over petroleum price and allocation decisions throughout the 1970's. It relies in large part on the many analyses and comments written by academics, government officials and others who have studied the topic over the past nine years. The first part of this study outlines the conditions in the domestic petroleum market that led to the imposition of first price, and then allocation controls on the oil industry. The second part explores the operation and effectiveness of the regulations during periods of oil supply disruptions - the Arab embargo of 1973-1974 and the 1979 shortfall attendant to the revolution in Iran. The third part describes many of the more important changes made to the regulations during non-disrupted periods, and examines the effect the regulations had on the operation and structure of the domestic oil industry. The last part analyzes the overall effects of the regulations on consumer prices and national welfare.

  19. Marine Hydrokinetic Energy Regulators Workshop: Lessons from Wind

    SciTech Connect (OSTI)

    Baring-Gould, E. Ian

    2015-09-03

    Ian Baring-Gould presented these lessons learned from wind energy to an audience of marine hydrokinetic regulators. Lessons learned spanned the areas of technology advances, using collaborative approaches to involve key stakeholders; using baseline studies to measure and prioritize wildlife impacts, and look at avoidance and mitigation options early in the process.

  20. Effects of new environmental regulations on coal-fired generation

    SciTech Connect (OSTI)

    LaCount, R.

    1999-07-01

    As restructuring of the electricity industry places downward pressure on power production costs, new environmental regulations are having the opposite effect. Although power plants may be subject to a variety of environmental regulations over the next ten years including reductions in mercury, toxics, and carbon dioxide, new regulations for sulfur dioxide (SO2) and nitrogen oxides (NOX) are poised to impact the electricity industry in the very short term. The cost for coal-fired power plants to comply with these new regulations has the potential to alter their competitive position. January 1, 2000 marks the beginning of Phase II for the Environmental Protection Agency's SO2 allowance market. Starting in January, all coal and oil plants above 25 MW will be required to comply with the federal SO2 provisions. Regulatory deadlines for NOX are also fast approaching; though the ultimate requirements are still subject to change. On May 1, 1999, a NOX allowance market began for states within the Northeast Ozone Transport Commission (OTC). A second phase of this program is scheduled to begin in 2003 that will lower the overall cap for allowable NOX emissions in the participating states. EPA is also working to expand the reach of regional NOX reductions in 2003 through its NOX SIP call. This program, which is currently subject to litigation, would require NOX reductions in 14 states outside of the OTC. A new study by Resource Data International (RDI), Coal-Fired Generation in Competitive Power Markets, assessed the potential impact that the new SO2 and NOX regulations may have on the competitiveness of coal-fired generation. Overall, the study shows that coal-fired generation will continue to grow despite significant environmental costs and competition from natural gas-fired units. The new environmental regulations have the effect of increasing the dispatch cost of coal-fired units from $0.65/MWh on average in the WSCC to $4.14/MWh on average in the MAAC region. The addition of environmental adders for SO2 and NOX pushes some coal-fired units above the fuel cost of combined cycle gas-fired units, especially those coal-fired units with poor heat rates and/or high delivered fuel costs. This presentation will review the current regulatory status of both the new SO2 and NOX regulations and address the key findings from RDI's new study.

  1. Arsenic-induced cutaneous hyperplastic lesions are associated with the dysregulation of Yap, a Hippo signaling-related protein

    SciTech Connect (OSTI)

    Li, Changzhao; Srivastava, Ritesh K.; Elmets, Craig A.; Afaq, Farrukh; Athar, Mohammad

    2013-09-06

    Highlights: Arsenic activates canonical Hippo signaling pathway and up-regulates ?Catenin in the skin. Arsenic activates transcriptional activity of Yap by its nuclear translocation. Yap is involved in the disruption of tight/adherens junctions in arsenic-exposed animals. -- Abstract: Arsenic exposure in humans causes a number of toxic manifestations in the skin including cutaneous neoplasm. However, the mechanism of these alterations remains elusive. Here, we provide novel observations that arsenic induced Hippo signaling pathway in the murine skin. This pathway plays crucial roles in determining organ size during the embryonic development and if aberrantly activated in adults, contributes to the pathogenesis of epithelial neoplasm. Arsenic treatment enhanced phosphorylation-dependent activation of LATS1 kinase and other Hippo signaling regulatory proteins Sav1 and MOB1. Phospho-LATS kinase is known to catalyze the inactivation of a transcriptional co-activator, Yap. However, in arsenic-treated epidermis, we did not observed its inactivation. Thus, as expected, unphosphorylated-Yap was translocated to the nucleus in arsenic-treated epidermis. Yap by binding to the transcription factors TEADs induces transcription of its target genes. Consistently, an up-regulation of Yap-dependent target genes Cyr61, Gli2, Ankrd1 and Ctgf was observed in the skin of arsenic-treated mice. Phosphorylated Yap is important in regulating tight and adherens junctions through its binding to ?Catenin. We found disruption of these junctions in the arsenic-treated mouse skin despite an increase in ?Catenin. These data provide evidence that arsenic-induced canonical Hippo signaling pathway and Yap-mediated disruption of tight and adherens junctions are independently regulated. These effects together may contribute to the carcinogenic effects of arsenic in the skin.

  2. Photoreactive synthetic regulator of protein function and methods of use thereof

    DOE Patents [OSTI]

    Trauner, Dirk; Isacoff, Ehud Y; Kramer, Richard H; Banghart, Matthew R; Fortin, Doris L; Mourot, Alexandre

    2015-03-31

    The present disclosure provides a photoreactive synthetic regulator of protein function. The present disclosure further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present disclosure further provides methods of modulating protein function, involving use of light.

  3. 49 CFR Subchapter C, Parts 171-177: Hazardous Materials Regulations

    Broader source: Energy.gov [DOE]

    The U.S. Department of Transportation Pipeline and Hazardous Materials Safety Administration regulates the transport of hazardous materials through Title 49 of the Code of Federal Regulations (49 CFR), Subchapter C, "Hazardous Materials Regulations." Parts 171-177 provide general information on hazardous materials and regulation for their packaging and their shipment by rail, air, vessel, and public highway.

  4. PKA regulates calcineurin function through the phosphorylation of RCAN1: Identification of a novel phosphorylation site

    SciTech Connect (OSTI)

    Kim, Seon Sook; Lee, Eun Hye; Lee, Kooyeon; Jo, Su-Hyun; Seo, Su Ryeon

    2015-04-17

    Calcineurin is a calcium/calmodulin-dependent phosphatase that has been implicated in T cell activation through the induction of nuclear factors of activated T cells (NFAT). We have previously suggested that endogenous regulator of calcineurin (RCAN1, also known as DSCR1) is targeted by protein kinase A (PKA) for the control of calcineurin activity. In the present study, we characterized the PKA-mediated phosphorylation site in RCAN1 by mass spectrometric analysis and revealed that PKA directly phosphorylated RCAN1 at the Ser 93. PKA-induced phosphorylation and the increase in the half-life of the RCAN1 protein were prevented by the substitution of Ser 93 with Ala (S93A). Furthermore, the PKA-mediated phosphorylation of RCAN1 at Ser 93 potentiated the inhibition of calcineurin-dependent pro-inflammatory cytokine gene expression by RCAN1. Our results suggest the presence of a novel phosphorylation site in RCAN1 and that its phosphorylation influences calcineurin-dependent inflammatory target gene expression. - Highlights: • We identify novel phosphorylation sites in RCAN1 by LC-MS/MS analysis. • PKA-dependent phosphorylation of RCAN1 at Ser 93 inhibits calcineurin-mediated intracellular signaling. • We show the immunosuppressive function of RCAN1 phosphorylation at Ser 93 in suppressing cytokine expression.

  5. Photoperiodic Regulation of the Seasonal Pattern of Photosynthetic Capacity and the Implications for Carbon Cycling

    SciTech Connect (OSTI)

    Bauerle, William L.; Oren, Ram; Way, Danielle A.; Qian, Song S.; Stoy, Paul C.; Thornton, Peter E; Bowden, Joseph D.; Hoffman, Forrest M; Reynolds, Robert F.

    2012-01-01

    Although temperature is an important driver of seasonal changes in photosynthetic physiology, photoperiod also regulates leaf activity. Climate change will extend growing seasons if temperature cues predominate, but photoperiod-controlled species will show limited responsiveness to warming. We show that photoperiod explains more seasonal variation in photosynthetic activity across 23 tree species than temperature. Although leaves remain green, photosynthetic capacity peaks just after summer solstice and declines with decreasing photoperiod, before air temperatures peak. In support of these findings, saplings grown at constant temperature but exposed to an extended photoperiod maintained high photosynthetic capacity, but photosynthetic activity declined in saplings experiencing a naturally shortening photoperiod; leaves remained equally green in both treatments. Incorporating a photoperiodic correction of photosynthetic physiology into a global-scale terrestrial carbon-cycle model significantly improves predictions of seasonal atmospheric CO{sub 2} cycling, demonstrating the benefit of such a function in coupled climate system models. Accounting for photoperiod-induced seasonality in photosynthetic parameters reduces modeled global gross primary production 2.5% ({approx}4 PgC y{sup -1}), resulting in a >3% ({approx}2 PgC y{sup -1}) decrease of net primary production. Such a correction is also needed in models estimating current carbon uptake based on remotely sensed greenness. Photoperiod-associated declines in photosynthetic capacity could limit autumn carbon gain in forests, even if warming delays leaf senescence.

  6. Magnetomechanically induced long period fiber gratings

    SciTech Connect (OSTI)

    Causado-Buelvas, Jesus D.; Gomez-Cardona, Nelson D.; Torres, Pedro

    2008-04-15

    In this work, we report a simple, flexible method to create long period fiber gratings mechanically by controlling the repulsion/attraction force between two magnets that pressing a plate with a periodic array of small glass cylinders to a short length of optical fiber. Via the photoelastic effect, the pressure points induce the required periodic refractive index modulation to create the LPFG. We found that the induced device exhibits spectral characteristics similar to those of other types of LPFG. As the optical properties of LPFGs are directly related to the nature of the applied perturbations, we show, to our knowledge for the frrst time, how is the evolution of birefringence effects in mechanically induced LPFGs.

  7. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |; Panozzo, J.; Libertin, C.R.

    1996-02-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.

  8. Apelin-13-induced proliferation and migration induced of rat vascular smooth muscle cells is mediated by the upregulation of Egr-1

    SciTech Connect (OSTI)

    Liu, Qi-Feng; Yu, Hong-Wei; You, Lu; Liu, Ming-Xin; Li, Ke-Yan; Department of Cardiology, Jinzhou Central Hospital, Jinzhou 121001 ; Tao, Gui-Zhou

    2013-09-20

    Highlights: The mechanism underlying the effects of Apelin-13 on VSMC was investigated. Apelin-13 induced VSMC migration, proliferation and Egr-1 and OPN upregulation. These effects were inhibited by the Egr-1 specific deoxyribozyme, ED5. The effects of Apelin-13 on VSMC are mediated via Egr-1 upregulation. These data will help in attempts to prevent and treat vascular remodeling diseases. -- Abstract: Apelin-13 plays an important role in the migration and proliferation of vascular smooth muscle cells (VSMCs); however, the underlying mechanisms are still unclear. Egr-1 is a nuclear transcription factor, which is considered to be the critical initiating factor of the processes of VSMC proliferation and migration. Egr-1 is known to regulate the expression of osteopontin (OPN), which is a marker of the phenotypic modulation that is a necessary condition of VSMC proliferation and migration. We hypothesized that the role of Apelin-13 is mediated via upregulation of Egr-1. To test this hypothesis, we analyzed the effects of Apelin-13 treatment on Egr-1 mRNA and protein expression in A10 rat aortic VSMCs by RT-PCR and Western blotting, respectively. Results showed that, Apelin-13 upregulated the expression of Egr-1. Furthermore, treatment with the extracellular-regulated protein kinase (ERK) inhibitor, PD98059, inhibited the upregulation of Egr-1 by Apelin-13. In addition, this upregulation was inhibited by treatment of VSMCs with the Egr-1 specific deoxyribozyme ED5 (DNAenzyme/10-23 DRz). Furthermore, ED5 treatment was found to significantly inhibit Apelin-13-induced migration and proliferation of VSMCs using transwell and MTT assays, respectively. The evaluation of OPN mRNA and protein expression levels by RT-PCR and Western blot analyses revealed that ED5 treatment also inhibited Apelin-13-induced OPN upregulation. The results of this study indicated that Apelin-13 upregulates Egr-1 via ERK. Furthermore, Apelin-13 induced the proliferation and migration of VSMCs as well as the upregulation of OPN via the upregulation of Egr-1. These results will provide an important theoretical and experimental basis for the control of inappropriate remodeling of vessel walls, and will hopefully lead to the prevention and treatment of vascular remodeling diseases.

  9. A conserved serine residue regulates the stability of Drosophila Salvador and human WW domain-containing adaptor 45 through proteasomal degradation

    SciTech Connect (OSTI)

    Wu, Di Wu, Shian

    2013-04-19

    Highlights: Ser-17 is key for the stability of Drosophila Sav. Ala mutation of Ser-17 promotes the proteasomal degradation of Sav. Ser-17 residue is not the main target of Hpo-induced Sav stabilization. Hpo-dependent and -independent mechanisms regulate Sav stability. This mechanism is conserved in the homologue of Sav, human WW45. -- Abstract: The Hippo (Hpo) pathway is a conserved tumor suppressor pathway that controls organ size through the coordinated regulation of apoptosis and proliferation. Drosophila Salvador (Sav), which limits organ size, is a core component of the Hpo pathway. In this study, Ser-17 was shown to be important for the stability of Sav. Alanine mutation of Ser-17 promoted the proteasomal degradation of Sav. Destabilization and stabilization of the Sav protein mediated by alanine mutation of Ser-17 and by Hpo, respectively, were independent of each other. This implies that the stability of Sav is controlled by two mechanisms, one that is Ser-17-dependent and Hpo-independent, and another that is Ser-17-independent and Hpo-dependent. These dual mechanisms also regulated the human counterpart of Drosophila Sav, WW domain-containing adaptor 45 (WW45). The conservation of this regulation adds to its significance in normal physiology and tumorigenesis.

  10. Down-regulation of kelch domain-containing F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to ultraviolet radiation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhang, Xuebin; Liu, Chang -Jun; Gou, Mingyue; Guo, Chunrong; Yang, Huijun

    2014-12-01

    Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis Kelch-domain containing F-box proteins, AtKFB01, -20, and -50, function as the negative regulators controlling phenylpropanoid biosynthesis via mediating PAL’s ubiquitination and subsequent degradation. Here, we reveal that Arabidopsis KFB39, a close homolog of AtKFB50, also interacts physically with PAL isozymes and modulates PALs' stability and activity. Disturbing the expression of KFB39 reciprocally affects the accumulation/deposition of a set of phenylpropanoid end products, suggesting thatmore » KFB39 is an additional post-translational regulator responsible for the turnover of PAL and negatively controlling phenylpropanoid biosynthesis. Furthermore, we discover that exposure of Arabidopsis to UV-B radiation suppresses the expression of all four KFB genes while inducing the transcription of PAL isogenes; these data suggest that Arabidopsis consolidates both transcriptional and post-translational regulation mechanisms to maximize its responses to UV stress. Simultaneous down-regulation of all four identified KFBs significantly enhances the production of (poly)phenols and the plant’s tolerance to UV irradiation. This study offers a biotechnological approach for engineering the production of useful phenolic chemicals and for increasing a plant’s resistance to environmental stress.« less

  11. Down-regulation of kelch domain-containing F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to ultraviolet radiation

    SciTech Connect (OSTI)

    Zhang, Xuebin [Brookhaven National Lab. (BNL), Upton, NY (United States); Shanxi Agriculture Univ., Taigu, Shanxi (People's Republic of China); Stony Brook Univ., Stony Brook, NY (United States); Liu, Chang -Jun [Brookhaven National Lab. (BNL), Upton, NY (United States); Shanxi Agriculture Univ., Taigu, Shanxi (People's Republic of China); Stony Brook Univ., Stony Brook, NY (United States); Gou, Mingyue [Brookhaven National Lab. (BNL), Upton, NY (United States); Shanxi Agriculture Univ., Taigu, Shanxi (People's Republic of China); Stony Brook Univ., Stony Brook, NY (United States); Guo, Chunrong [Brookhaven National Lab. (BNL), Upton, NY (United States); Shanxi Agriculture Univ., Taigu, Shanxi (People's Republic of China); Stony Brook Univ., Stony Brook, NY (United States); Yang, Huijun [Brookhaven National Lab. (BNL), Upton, NY (United States); Shanxi Agriculture Univ., Taigu, Shanxi (People's Republic of China); Stony Brook Univ., Stony Brook, NY (United States)

    2014-12-01

    Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis Kelch-domain containing F-box proteins, AtKFB01, -20, and -50, function as the negative regulators controlling phenylpropanoid biosynthesis via mediating PALs ubiquitination and subsequent degradation. Here, we reveal that Arabidopsis KFB39, a close homolog of AtKFB50, also interacts physically with PAL isozymes and modulates PALs' stability and activity. Disturbing the expression of KFB39 reciprocally affects the accumulation/deposition of a set of phenylpropanoid end products, suggesting that KFB39 is an additional post-translational regulator responsible for the turnover of PAL and negatively controlling phenylpropanoid biosynthesis. Furthermore, we discover that exposure of Arabidopsis to UV-B radiation suppresses the expression of all four KFB genes while inducing the transcription of PAL isogenes; these data suggest that Arabidopsis consolidates both transcriptional and post-translational regulation mechanisms to maximize its responses to UV stress. Simultaneous down-regulation of all four identified KFBs significantly enhances the production of (poly)phenols and the plants tolerance to UV irradiation. This study offers a biotechnological approach for engineering the production of useful phenolic chemicals and for increasing a plants resistance to environmental stress.

  12. Down-regulation of kelch domain-containing F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to ultraviolet radiation

    SciTech Connect (OSTI)

    Zhang, Xuebin; Liu, Chang -Jun; Gou, Mingyue; Guo, Chunrong; Yang, Huijun

    2014-12-01

    Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis Kelch-domain containing F-box proteins, AtKFB01, -20, and -50, function as the negative regulators controlling phenylpropanoid biosynthesis via mediating PAL’s ubiquitination and subsequent degradation. Here, we reveal that Arabidopsis KFB39, a close homolog of AtKFB50, also interacts physically with PAL isozymes and modulates PALs' stability and activity. Disturbing the expression of KFB39 reciprocally affects the accumulation/deposition of a set of phenylpropanoid end products, suggesting that KFB39 is an additional post-translational regulator responsible for the turnover of PAL and negatively controlling phenylpropanoid biosynthesis. Furthermore, we discover that exposure of Arabidopsis to UV-B radiation suppresses the expression of all four KFB genes while inducing the transcription of PAL isogenes; these data suggest that Arabidopsis consolidates both transcriptional and post-translational regulation mechanisms to maximize its responses to UV stress. Simultaneous down-regulation of all four identified KFBs significantly enhances the production of (poly)phenols and the plant’s tolerance to UV irradiation. This study offers a biotechnological approach for engineering the production of useful phenolic chemicals and for increasing a plant’s resistance to environmental stress.

  13. The endocrine disruptor diethylstilbestrol induces adipocyte differentiation and promotes obesity in mice

    SciTech Connect (OSTI)

    Hao, Chan-Juan; Cheng, Xue-Jia; Xia, Hong-Fei Ma, Xu

    2012-08-15

    Epidemiology studies indicate that exposure to endocrine disruptors during developmental “window” contributes to adipogenesis and the development of obesity. Implication of endocrine disruptor such as diethylstilbestrol (DES) on adipose tissue development has been poorly investigated. Here we evaluated the effects of DES on adipocyte differentiation in vitro and in vivo, and explored potential mechanism involved in its action. DES induced 3T3-L1 preadipocyte differentiation in a dose-dependent manner, and activated the expression of estrogen receptor (ER) and peroxisome proliferator-acivated receptor (PPAR) γ as well as its target genes required for adipogenesis in vitro. ER mediated the enhancement of DES-induced PPARγ activity. Moreover, DES perturbed key regulators of adipogenesis and lipogenic pathway in vivo. In utero exposure to low dose of DES significantly increased body weight, liver weight and fat mass in female offspring at postnatal day (PND) 60. In addition, serum triglyceride and glucose levels were also significantly elevated. These results suggest that perinatal exposure to DES may be expected to increase the incidence of obesity in a sex-dependent manner and can act as a potential chemical stressor for obesity and obesity-related disorders. -- Highlights: ► DES induced adipocyte differentiation in a dose-dependent manner in 3T3-L1 cells. ► DES activated adipogenic critical regulators and markers in vitro and in vivo. ► Perinatal exposure to DES led to the obese phenotype in female offspring. ► DES might be a potential chemical stressor for obesity and obesity-related disorders.

  14. Legal nature of LPG (liquefied petroleum gas) regulation

    SciTech Connect (OSTI)

    Liddell, G.

    1986-08-01

    The commercial exploitation of Liquefied Petroleum Gas (LPG) in New Zealand has occurred without a particular and comprehensive concern for any legal implications. The paper in Part I examines definitional questions, assesses in Part II the ability of courts and quasi-courts to evaluate risks associated with the product, examines in Part III the utility of common law remedies for injuries or associated with or arising from LPG, analyzes in Part IV the statutory regulation of LPG, concentrating particularly on the Dangerous Goods (Class 2 - Gases) Regulations 1980, discusses in Part V recent planning case-law concerning LPG development, and concludes that some reform is necessary to produce a more-coherent and precise regulatory regime that takes into account both the needs of developers and those affected by the development of LPG.

  15. The potassium ion channel opener NS1619 inhibits proliferation and induces apoptosis in A2780 ovarian cancer cells

    SciTech Connect (OSTI)

    Han Xiaobing; Xi Ling; Wang Hui; Huang Xiaoyuan; Ma Xiangyi; Han Zhiqiang; Wu Peng; Ma Xiaoli; Lu Yunping; Wang, Gang Zhou Jianfeng; Ma Ding

    2008-10-17

    Diverse types of voltage-gated potassium (K{sup +}) channels have been shown to be involved in regulation of cell proliferation. The maxi-conductance Ca{sup 2+}-activated K{sup +} channels (BK channels) may play an important role in the progression of human cancer. To explore the role of BK channels in regulation of apoptosis in human ovarian cancer cells, the effects of the specific BK channel activator NS1619 on induction of apoptosis in A2780 cells were observed. Following treatment with NS1619, cell proliferation was measured by MTT assay. Apoptosis of A2780 cells pretreated with NS1619 was detected by agarose gel electrophoresis of cellular DNA and flow cytometry. Our data demonstrate that NS1619 inhibits the proliferation of A2780 cells in a dosage and time dependent manner IC{sub 50} = 31.1 {mu}M, for 48 h pretreatment and induces apoptosis. Western blot analyses showed that the anti-proliferation effect of NS1619 was associated with increased expression of p53, p21, and Bax. These results indicate that BK channels play an important role in regulating proliferation of human ovarian cancer cells and may induce apoptosis through induction of p21{sup Cip1} expression in a p53-dependent manner.

  16. Regulators caught in fierce crossfire on new generation

    SciTech Connect (OSTI)

    2008-07-15

    Efforts of several utilities to construct new power plants not based on conventional coal but rather coal-gasification technology or nuclear fission are described. Several have been canceled because of rising costs. State regulators are faced with trying to protect customers from high rates and unsound investments by utilities. Critics maintain that ratepayers should not be forced to pay higher rates for plants that may never be built.

  17. Enforcement Regulations and Directives - Security | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Security Enforcement Regulations and Directives - Security Classified Information Security 10 C.F.R Part 824 - Procedural Rules for the Assessment of Civil Penalties for Classified Information Security Violations 10 C.F.R Part 1016 - Safeguarding of Restricted Data 10 C.F.R Part 1045 - Nuclear Classification and Declassification DOE Directives and NNSA Policy Letters Enforceable under 10 C.F.R. Part 824

  18. New insights into regulating the gut's microbial community | Argonne

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    National Laboratory insights into regulating the gut's microbial community August 27, 2015 Tweet EmailPrint The human body is home to billions of microbial cells, a number roughly three to 10 times greater than the number of human cells. Many of these microbes reside in the gastrointestinal system, or gut, and form a symbiotic relationship with their human hosts. These "commensal" organisms do not causes illness and in fact provide us with crucial metabolic and nutritional benefits

  19. NREL: Technology Deployment - Fleets Regulated by the Energy Policy Act

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fleets Regulated by the Energy Policy Act NREL supports the U.S. Department of Energy's (DOE) regulatory activities involving requirements under the Energy Policy Act (EPAct) for federal, state, and alternative fuel provider fleets. Congress passed EPAct in 1992 to reduce U.S. petroleum consumption through the use of alternative fuels, alternative fuel vehicles (AFVs), and other petroleum-displacement methods. DOE's primary means of achieving this goal in the transportation sector has been to

  20. DOE's Program Regulating Liquefied Natural Gas Export Applications |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 19, 2013 - 2:52pm Addthis Statement of Christopher Smith, Acting Assistant Secretary for Fossil Energy before the House Committee on Oversight and Government Reform, Subcommittee on Energy Policy, Health Care, and Entitlements View the archived Congressional Hearing on YouTube Thank you Chairman Lankford, Ranking Member Speier, and members of the Committee; I appreciate the opportunity to be here today to discuss the Department of Energy's (DOE) program regulating the

  1. DOE's Program Regulating Liquefied Natural Gas Export Applications |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 25, 2014 - 2:49pm Addthis Statement of Paula Gant, Deputy Assistant Secretary for Oil and Natural Gas, Before the House Subcommittee on Energy and Power, Committee on Energy and Commerce Thank you Chairman Whitfield, Ranking Member Rush, and members of the Subcommittee; I appreciate the opportunity to be here today to discuss the Department of Energy's (DOE) program regulating the export of liquefied natural gas (LNG), and to answer questions about H.R. 6, the

  2. Application of EPA regulations to low-level radioactive waste

    SciTech Connect (OSTI)

    Bowerman, B.S.; Piciulo, P.L.

    1985-01-01

    The survey reported here was conducted with the intent of identifying categories of low-level radioactive wastes which would be classified under EPA regulations 40 CFR Part 261 as hazardous due to the chemical properties of the waste. Three waste types are identified under these criteria as potential radioactive mixed wastes: wastes containing organic liquids; wastes containing lead metal; and wastes containing chromium. The survey also indicated that certain wastes, specific to particular generators, may also be radioactive mixed wastes. Ultimately, the responsibility for determining whether a facility's wastes are mixed wastes rest with the generator. However, the uncertainties as to which regulations are applicable, and the fact that no legal definition of mixed wastes exists, make such a determination difficult. In addition to identifying mixed wastes, appropriate methods for the management of mixed wastes must be defined. In an ongoing study, BNL is evaluating options for the management of mixed wastes. These options will include segregation, substitution, and treatments to reduce or eliminate chemical hazards associated with the wastes listed above. The impacts of the EPA regulations governing hazardous wastes on radioactive mixed waste cannot be assessed in detail until the applicability of these regulations is agreed upon. This issue is still being discussed by EPA and NRC and should be resolved in the near future. Areas of waste management which may affect generators of mixed wastes include: monitoring/tracking of wastes before shipment; chemical testing of wastes; permits for treatment of storage of wastes; and additional packaging requirements. 3 refs., 1 fig., 2 tabs.

  3. Regulation Services with Demand Response - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regulation Services with Demand Response Pacific Northwest National Laboratory Contact PNNL About This Technology Using grid frequency information, researchers have created algorithms that intelligently control power demand while meeting consumer objectives (i.e. target pricing). Using grid frequency information, researchers have created algorithms that intelligently control power demand while meeting consumer objectives (i.e. target pricing). Technology Marketing Summary Grid Friendly(tm)

  4. Code of Federal Regulations Occupational Radiation Protection; Final Rule |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Occupational Radiation Protection; Final Rule Code of Federal Regulations Occupational Radiation Protection; Final Rule The Department of Energy (DOE) is amending its primary standards for occupational radiation protection. This final rule is the culmination of a systematic analysis to identify the elements of a comprehensive radiation protection program and determine those elements of such a program that should be codified as DOE continues its transition from a system

  5. Environmental regulation of coal mining. SMCRA`s Second Decade

    SciTech Connect (OSTI)

    McElfish, J.M.; Beier, A.E.

    1990-12-31

    If you find yourself involved in the workings of the Surface Mining Control and Reclamation Act of 1977 (SMCRA), let ELI`s guide take you through SMCRA`s second decade. Environmental Regulation of Coal mining: SMCRA`s Second Decade gives you a clear picture of SMCRA`s statutory and regulatory requirements, and identifies the key policy disputes and problems that will be confronted in the years ahead.

  6. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific andmore » genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.« less

  7. DC switching regulated power supply for driving an inductive load

    DOE Patents [OSTI]

    Dyer, G.R.

    1983-11-29

    A dc switching regulated power supply for driving an inductive load is provided. The regulator basic circuit is a bridge arrangement of diodes and transistors. First and second opposite legs of the bridge are formed by first and second parallel-connected transistor arrays, respectively, while the third and fourth legs of the bridge are formed by appropriately connected first and second parallel connected diode arrays, respectively. A dc power supply is connected to the input of the bridge and the output is connected to the load. A servo controller is provided to control the switching rate of the transistors to maintain a desired current to the load. The regulator may be operated in three stages or modes: (1) for current runup in the load, both first and second transistor switch arrays are turned on and current is supplied to the load through both transistor arrays. (2) When load current reaches the desired level, the first switch is turned off, and load current flywheels through the second switch array and the fourth leg diode array connecting the second switch array in series with the load. Current is maintained by alternating between modes 1 and 2 at a suitable duty cycle and switching rate set by the controller. (3) Rapid current rundown is accomplished by turning both switch arrays off, allowing load current to be dumped back into the source through the third and fourth diode arrays connecting the source in series opposition with the load to recover energy from the inductive load.

  8. DC switching regulated power supply for driving an inductive load

    DOE Patents [OSTI]

    Dyer, George R.

    1986-01-01

    A power supply for driving an inductive load current from a dc power supply hrough a regulator circuit including a bridge arrangement of diodes and switching transistors controlled by a servo controller which regulates switching in response to the load current to maintain a selected load current. First and second opposite legs of the bridge are formed by first and second parallel-connected transistor arrays, respectively, while the third and fourth legs of the bridge are formed by appropriately connected first and second parallel connected diode arrays, respectively. The regulator may be operated in three "stages" or modes: (1) For current runup in the load, both first and second transistor switch arrays are turned "on" and current is supplied to the load through both transistor arrays. (2) When load current reaches the desired level, the first switch is turned "off", and load current "flywheels" through the second switch array and the fourth leg diode array connecting the second switch array in series with the load. Current is maintained by alternating between modes 1 and 2 at a suitable duty cycle and switching rate set by the controller. (3) Rapid current rundown is accomplished by turning both switch arrays "off", allowing load current to be dumped back into the source through the third and fourth diode arrays connecting the source in series opposition with the load to recover energy from the inductive load. The three operating states are controlled automatically by the controller.

  9. Comparative genomics of transcriptional regulation of methionine metabolism in proteobacteria

    SciTech Connect (OSTI)

    Leyn, Semen A.; Suvorova, Inna A.; Kholina, Tatiana D.; Sherstneva, Sofia S.; Novichkov, Pavel S.; Gelfand, Mikhail S.; Rodionov, Dmitry A.; Kuipers, Oscar P.

    2014-11-20

    Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ~200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.

  10. Soluble FGFR4 extracellular domain inhibits FGF19-induced activation of FGFR4 signaling and prevents nonalcoholic fatty liver disease

    SciTech Connect (OSTI)

    Chen, Qiang; The First Affiliated Hospital of Xiamen University, Xiamen ; Jiang, Yuan; An, Yuan; Zhao, Na; Zhao, Yang; Yu, Chundong

    2011-06-17

    Highlights: {yields} Soluble FGFR4 extracellular domain (FGFR4-ECD) was effectively expressed. {yields} FGFR4-ECD inhibited FGF19-induced activation of FGFR4 signaling. {yields} FGFR4-ECD reduced palmitic acid-induced steatosis of HepG2 cells. {yields} FGFR4-ECD reduced tetracycline-induced fatty liver in mice. {yields} FGFR4-ECD partially restored tetracycline-repressed PPAR{alpha} expression. -- Abstract: Fibroblast growth factor receptor 4 (FGFR4) is a transmembrane tyrosine kinase receptor that plays a crucial role in the regulation of hepatic bile acid and lipid metabolism. FGFR4 underlies high-fat diet-induced hepatic steatosis, suggesting that inhibition of FGFR4 activation may be an effective way to prevent or treat nonalcoholic fatty liver disease (NAFLD). To determine whether neutralization of FGFR4 ligands by soluble FGFR4 extracellular domain (FGFR4-ECD) can inhibit the activation of FGFR4, we constructed FGFR4-ECD expression vector and showed that FGFR4-ECD was effectively expressed in cells and secreted into culture medium. FGFR4-ECD inhibited FGF19-induced activation of FGFR4 signaling and reduced steatosis of HepG2 induced by palmitic acid in vitro. Furthermore, in a tetracycline-induced fatty liver model, expression of FGFR4-ECD in mouse liver reduced the accumulation of hepatic lipids and partially restored the expression of peroxisome proliferator-activated receptor {alpha} (PPAR{alpha}), which promotes the mitochondrial fatty acid beta-oxidation but is repressed by tetracycline. Taken together, these results demonstrate that FGFR4-ECD can block FGFR4 signaling and prevent hepatic steatosis, highlighting the potential value of inhibition of FGFR4 signaling as a method for therapeutic intervention against NAFLD.

  11. Low doses ionizing radiation enhances the invasiveness of breast cancer cells by inducing epithelial-mesenchymal transition

    SciTech Connect (OSTI)

    Zhang, Xin; Li, Xiaoyan; Zhang, Ning; Yang, Qifeng; Moran, Meena S.

    2011-08-19

    Highlights: {yields} Low doses ionizing irradiation would enhance the invasiveness of breast cancer cells by inducing EMT. {yields} Low doses ionizing radiation induced morphologic changes in breast cancer cells. {yields} Low doses ionizing radiation led to upregulation of mesenchymal markers and down-regulation of epithelial markers. {yields} Low doses ionizing radiation increased migration and invasion of breast cancer cells. -- Abstract: Epithelial-mesenchymal transition (EMT) is a process cellular morphologic and molecular alterations facilitate cell invasion. We hypothesized that low dose ionizing irradiation (LDIR) enhances the invasiveness of breast cancer cells by inducing EMT. The effects of LDIR on cellular morphology and the EMT markers of MCF-7 breast cancer cells were analyzed by western blot/RT-PCR and migration/invasion was examined using the transwell assay. We found that LDIR led to the phenotypic changes of EMT in MCF-7 cells and down-regulation of epithelial differentiation markers and transcriptional induction of mesenchymal markers. Furthermore, the radiated cells demonstrated enhanced migration/invasion MCF-7 cells compared with non-radiated cells. In summary, LDIR promotes the invasiveness of breast cancer cells through epithelial to mesenchymal transition. These findings may ultimately provide a new targeted approach for improving the therapeutic effectiveness of radiation in breast cancer.

  12. A biphasic endothelial stress-survival mechanism regulates the cellular response to vascular endothelial growth factor A

    SciTech Connect (OSTI)

    Latham, Antony M.; Odell, Adam F.; Mughal, Nadeem A.; Issitt, Theo; Ulyatt, Clare; Walker, John H.; Homer-Vanniasinkam, Shervanthi; Ponnambalam, Sreenivasan

    2012-11-01

    Vascular endothelial growth factor A (VEGF-A) is an essential cytokine that regulates endothelial function and angiogenesis. VEGF-A binding to endothelial receptor tyrosine kinases such as VEGFR1 and VEGFR2 triggers cellular responses including survival, proliferation and new blood vessel sprouting. Increased levels of a soluble VEGFR1 splice variant (sFlt-1) correlate with endothelial dysfunction in pathologies such as pre-eclampsia; however the cellular mechanism(s) underlying the regulation and function of sFlt-1 are unclear. Here, we demonstrate the existence of a biphasic stress response in endothelial cells, using serum deprivation as a model of endothelial dysfunction. The early phase is characterized by a high VEGFR2:sFlt-1 ratio, which is reversed in the late phase. A functional consequence is a short-term increase in VEGF-A-stimulated intracellular signaling. In the late phase, sFlt-1 is secreted and deposited at the extracellular matrix. We hypothesized that under stress, increased endothelial sFlt-1 levels reduce VEGF-A bioavailability: VEGF-A treatment induces sFlt-1 expression at the cell surface and VEGF-A silencing inhibits sFlt-1 anchorage to the extracellular matrix. Treatment with recombinant sFlt-1 inhibits VEGF-A-stimulated in vitro angiogenesis and sFlt-1 silencing enhances this process. In this response, increased VEGFR2 levels are regulated by the phosphatidylinositol-3-kinase and PKB/Akt signaling pathways and increased sFlt-1 levels by the ERK1/2 signaling pathway. We conclude that during serum withdrawal, cellular sensing of environmental stress modulates sFlt-1 and VEGFR2 levels, regulating VEGF-A bioavailability and ensuring cell survival takes precedence over cell proliferation and migration. These findings may underpin an important mechanism contributing to endothelial dysfunction in pathological states. -- Highlights: Black-Right-Pointing-Pointer Endothelial cells mount a stress response under conditions of low serum. Black-Right-Pointing-Pointer Endothelial VEGFR levels are modulated during this response. Black-Right-Pointing-Pointer The cell regulates VEGF-A bioavailability and cell survival. Black-Right-Pointing-Pointer This may partly underlie endothelial dysfunction seen in many pathologies.

  13. Structurally distinct polycyclic aromatic hydrocarbons induce differential transcriptional responses in developing zebrafish

    SciTech Connect (OSTI)

    Goodale, Britton C.; Tilton, Susan C.; Corvi, Margaret M.; Wilson, Glenn R.; Janszen, Derek B.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-11-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment as components of fossil fuels and by-products of combustion. These multi-ring chemicals differentially activate the aryl hydrocarbon receptor (AHR) in a structurally dependent manner, and induce toxicity via both AHR-dependent and -independent mechanisms. PAH exposure is known to induce developmental malformations in zebrafish embryos, and recent studies have shown cardiac toxicity induced by compounds with low AHR affinity. Unraveling the potentially diverse molecular mechanisms of PAH toxicity is essential for understanding the hazard posed by complex PAH mixtures present in the environment. We analyzed transcriptional responses to PAH exposure in zebrafish embryos exposed to benz(a)anthracene (BAA), dibenzothiophene (DBT) and pyrene (PYR) at concentrations that induced developmental malformations by 120 h post-fertilization (hpf). Whole genome microarray analysis of mRNA expression at 24 and 48 hpf identified genes that were differentially regulated over time and in response to the three PAH structures. PAH body burdens were analyzed at both time points using GCMS, and demonstrated differences in PAH uptake into the embryos. This was important for discerning dose-related differences from those that represented unique molecular mechanisms. While BAA misregulated the least number of transcripts, it caused strong induction of cyp1a and other genes known to be downstream of the AHR, which were not induced by the other two PAHs. Analysis of functional roles of misregulated genes and their predicted regulatory transcription factors also distinguished the BAA response from regulatory networks disrupted by DBT and PYR exposure. These results indicate that systems approaches can be used to classify the toxicity of PAHs based on the networks perturbed following exposure, and may provide a path for unraveling the toxicity of complex PAH mixtures. - Highlights: Defined global mRNA expression changes induced by developmental exposure to PAHs Determined PAH body burdens following developmental exposure Genes uniquely induced by benz(a)anthracene included targets of the AHR and RELA Dibenzothiophene and pyrene perturbed a distinct RELA network Transcriptional networks reveal differential mechanisms of PAH toxicity.

  14. DOE Releases Updated Induced Seismicity Protocol

    Broader source: Energy.gov [DOE]

    At the 37th Stanford Geothermal Workshop in Stanford, California, the Geothermal Technologies Program at the U.S. Department of Energy (DOE) released an updated Induced Seismicity Protocol. This document supplements the existing International Energy Agency (IEA) protocol of 2009, and is intended to be a living document kept up-to-date with state-of-the-art knowledge and practices.

  15. RNA Helicase DDX5 Regulates MicroRNA Expression and Contributes to Cytoskeletal Reorganization in Basal Breast Cancer Cells

    SciTech Connect (OSTI)

    Wang, Daojing; Huang, Jing; Hu, Zhi

    2011-11-15

    RNA helicase DDX5 (also p68) is involved in all aspects of RNA metabolism and serves as a transcriptional co-regulator, but its functional role in breast cancer remains elusive. Here, we report an integrative biology study of DDX5 in breast cancer, encompassing quantitative proteomics, global MicroRNA profiling, and detailed biochemical characterization of cell lines and human tissues. We showed that protein expression of DDX5 increased progressively from the luminal to basal breast cancer cell lines, and correlated positively with that of CD44 in the basal subtypes. Through immunohistochemistry analyses of tissue microarrays containing over 200 invasive human ductal carcinomas, we observed that DDX5 was upregulated in the majority of malignant tissues, and its expression correlated strongly with those of Ki67 and EGFR in the triple-negative tumors. We demonstrated that DDX5 regulated a subset of MicroRNAs including miR-21 and miR-182 in basal breast cancer cells. Knockdown of DDX5 resulted in reorganization of actin cytoskeleton and reduction of cellular proliferation. The effects were accompanied by upregulation of tumor suppressor PDCD4 (a known miR-21 target); as well as upregulation of cofilin and profilin, two key proteins involved in actin polymerization and cytoskeleton maintenance, as a consequence of miR-182 downregulation. Treatment with miR-182 inhibitors resulted in morphologic phenotypes resembling those induced by DDX5 knockdown. Using bioinformatics tools for pathway and network analyses, we confirmed that the network for regulation of actin cytoskeleton was predominantly enriched for the predicted downstream targets of miR-182. Our results reveal a new functional role of DDX5 in breast cancer via the DDX5?miR-182?actin cytoskeleton pathway, and suggest the potential clinical utility of DDX5 and its downstream MicroRNAs in the theranostics of breast cancer.

  16. Cobalt chloride decreases fibroblast growth factor-21 expression dependent on oxidative stress but not hypoxia-inducible factor in Caco-2 cells

    SciTech Connect (OSTI)

    Liu, Yanlong; Department of Medicine, University of Louisville, Louisville, KY ; Wang, Chunhong; Department of Medicine, University of Louisville, Louisville, KY ; Wang, Yuhua; Department of Medicine, University of Louisville, Louisville, KY ; Ma, Zhenhua; Department of Medicine, University of Louisville, Louisville, KY ; Xiao, Jian; McClain, Craig; Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY; Alcohol Research Center, University of Louisville, Louisville, KY; Robley Rex Veterans Affairs Medical Center, Louisville, KY ; Li, Xiaokun; Feng, Wenke; Department of Medicine, University of Louisville, Louisville, KY; Alcohol Research Center, University of Louisville, Louisville, KY

    2012-10-15

    Fibroblast growth factor-21 (FGF21) is a potential metabolic regulator with multiple beneficial effects on metabolic diseases. FGF21 is mainly expressed in the liver, but is also found in other tissues including the intestine, which expresses β-klotho abundantly. The intestine is a unique organ that operates in a physiologically hypoxic environment, and is responsible for the fat absorption processes including triglyceride breakdown, re-synthesis and absorption into the portal circulation. In the present study, we investigated the effects of hypoxia and the chemical hypoxia inducer, cobalt chloride (CoCl{sub 2}), on FGF21 expression in Caco-2 cells and the consequence of fat accumulation. Physical hypoxia (1% oxygen) and CoCl{sub 2} treatment decreased both FGF21 mRNA and secreted protein levels. Gene silence and inhibition of hypoxia-inducible factor-α (HIFα) did not affect the reduction of FGF21 mRNA and protein levels by hypoxia. However, CoCl{sub 2} administration caused a significant increase in oxidative stress. The addition of n-acetylcysteine (NAC) suppressed CoCl{sub 2}-induced reactive oxygen species (ROS) formation and completely negated CoCl{sub 2}-induced FGF21 loss. mRNA stability analysis demonstrated that the CoCl{sub 2} administration caused a remarkable reduction in FGF21 mRNA stability. Furthermore, CoCl{sub 2} increased intracellular triglyceride (TG) accumulation, along with a reduction in mRNA levels of lipid lipase, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), and an increase of sterol regulatory element-binding protein-1c (SREBP1c) and stearoyl-coenzyme A (SCD1). Addition of both NAC and recombinant FGF21 significantly attenuated the CoCl{sub 2}-induced TG accumulation. In conclusion, the decrease of FGF21 in Caco-2 cells by chemical hypoxia is independent of HIFα, but dependent on an oxidative stress-mediated mechanism. The regulation of FGF21 by hypoxia may contribute to intestinal lipid metabolism and absorption. -- Graphical abstract: Physical and chemical hypoxia decrease FGF-21 expression, which is inhibited by antioxidant, N-acetyl cysteine (NAC), in Caco-2 cells. Highlights: ► Hypoxia down-regulates FGF21 expression in Caco-2 cells. ► FGF21 down-regulation is HIF-α independent. ► FGF21 down-regulation is modulated by oxidative stress-mediated mRNA stability. ► FGF21 is involved in hypoxia‐induced triglyceride accumulation in Caco-2 cells.

  17. Sulphoraphane, a naturally occurring isothiocyanate induces apoptosis in breast cancer cells by targeting heat shock proteins

    SciTech Connect (OSTI)

    Sarkar, Ruma; Mukherjee, Sutapa [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India); Biswas, Jaydip [Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India); Roy, Madhumita, E-mail: mitacnci@yahoo.co.in [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)] [Department of Environmental Carcinogenesis and Toxicology, Chittaranjan National Cancer Institute, 37, SP Mukherjee Road, Kolkata 700 026 (India)

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer HSPs (27, 70 and 90) and HSF1 are overexpressed in MCF-7 and MDA-MB-231 cells. Black-Right-Pointing-Pointer Sulphoraphane, a natural isothiocyanate inhibited HSPs and HSF1 expressions. Black-Right-Pointing-Pointer Inhibition of HSPs and HSF1 lead to regulation of apoptotic proteins. Black-Right-Pointing-Pointer Alteration of apoptotic proteins activate of caspases particularly caspase 3 and 9 leading to induction of apoptosis. Black-Right-Pointing-Pointer Alteration of apoptotic proteins induce caspases leading to induction of apoptosis. -- Abstract: Heat shock proteins (HSPs) are involved in protein folding, aggregation, transport and/or stabilization by acting as a molecular chaperone, leading to inhibition of apoptosis by both caspase dependent and/or independent pathways. HSPs are overexpressed in a wide range of human cancers and are implicated in tumor cell proliferation, differentiation, invasion and metastasis. HSPs particularly 27, 70, 90 and the transcription factor heat shock factor1 (HSF1) play key roles in the etiology of breast cancer and can be considered as potential therapeutic target. The present study was designed to investigate the role of sulphoraphane, a natural isothiocyanate on HSPs (27, 70, 90) and HSF1 in two different breast cancer cell lines MCF-7 and MDA-MB-231 cells expressing wild type and mutated p53 respectively, vis-a-vis in normal breast epithelial cell line MCF-12F. It was furthermore investigated whether modulation of HSPs and HSF1 could induce apoptosis in these cells by altering the expressions of p53, p21 and some apoptotic proteins like Bcl-2, Bax, Bid, Bad, Apaf-1 and AIF. Sulphoraphane was found to down-regulate the expressions of HSP70, 90 and HSF1, though the effect on HSP27 was not pronounced. Consequences of HSP inhibition was upregulation of p21 irrespective of p53 status. Bax, Bad, Apaf-1, AIF were upregulated followed by down-regulation of Bcl-2 and this effect was prominent in MCF-7 than in MDA-MB-231. However, very little change in the expression of Bid was observed. Alteration in Bcl-2 Bax ratio resulted in the release of cytochrome c from mitochondria and activation of caspases 3 and 9 which are in agreement with apoptotic index values. Sulphoraphane therefore can be regarded as a potent inducer of apoptosis due to HSP modulation in breast cancer cells.

  18. Price Incentivised Electric Vehicle Charge Control for Community Voltage Regulation

    SciTech Connect (OSTI)

    Kelly, Damian; Baroncelli, Fabio; Fowler, Christopher; Boundy, David; Pratt, Annabelle

    2014-11-03

    With the growing availability of Electric Vehicles, there is a significant opportunity to use battery 'smart-charging' for voltage regulation. This work designs and experimentally evaluates a system for price-incentivised electric vehicle charging. The system is designed to eliminate negative impacts to the user while minimising the cost of charging and achieving a more favourable voltage behaviour throughout the local grid over time. The practical issues associated with a real-life deployment are identified and resolved. The efficacy of the system is evaluated in the challenging scenario in which EVs are deployed in six closely distributed homes, serviced by the same low voltage residential distribution feeder.

  19. Enforcement Regulations and Directives - Nuclear Safety | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Nuclear Safety Enforcement Regulations and Directives - Nuclear Safety 10 C.F.R. Part 820 and Amendments 10 C.F.R. Part 820 - Procedural Rules for DOE Nuclear Activities 10 C.F.R. Part 820 - Procedural Rules for DOE Nuclear Activities; General Statement of Enforcement Policy; Final rule; amendment of enforcement policy statement and confirmation of interim rule Enforcement Guidance Supplements 10 C.F.R. Part 830 10 C.F.R. Part 830 - Nuclear Safety Management; Final Rule Office of

  20. Department of Energy Federal Acquisition Regulation Clause Usage Guide

    Broader source: Energy.gov [DOE]

    Attached for your information is a corrected Department of Energy Federal Acquisition Regulation Clause Usage Guide. This corrected clause matrix is also being posted to the Stripes library. The earlier edition incorrectly designated 52.223-4 Recovered Material Certification and 52.223-9 Estimate of Percentage of Recovered Material Content for EPA-Designated Items, as Not Applicable under management and operating contracts and other facility management contracts. They are actually required clauses as 52.223-17 requires the use of such products in service and construction contracts. They are also being designated as required under the service and construction contract columns.

  1. Structure, Function and Regulation of the Clostridium cellulovorans Cellulosome

    SciTech Connect (OSTI)

    Doi, Roy H

    2008-06-01

    Our major goal for this project (2004-2008) was to obtain an understanding ofthe structure, function, and regulation of the Clostridium cellulovorans cellulosomes. Our specific goals were to select genes for cellulosomal and non-cellulosomal enzymes and characterize their products, to study the synergistic action between cellulosomal and non-cellulosomal enzymes, to study the composition of cellulosomes when cells were grown with different carbon sources, continue our studies on the scaffolding protein and examine heterologous expression of cellulosomal genes in Bacillus subtilis. We fulfilled the specific goals of our proposal.

  2. REGULATION OF CARBON SEQUESTRATION AND WATER USE IN A OZARK FOREST...

    Office of Scientific and Technical Information (OSTI)

    REGULATION OF CARBON SEQUESTRATION AND WATER USE IN A OZARK FOREST: PROPOSING A NEW ... Title: REGULATION OF CARBON SEQUESTRATION AND WATER USE IN A OZARK FOREST: PROPOSING A NEW ...

  3. 2 CCR 402-10 - Rules and Regulations for Geothermal Well Permitting...

    Open Energy Info (EERE)

    2 CCR 402-10 - Rules and Regulations for Geothermal Well PermittingLegal Abstract Sets forth regulation for issuance of geothermal well permits by the Division of Water Resources....

  4. Division of Natural Gas Regulation (Import/Export) - E-Filing...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Division of Natural Gas Regulation (ImportExport) - E-Filing Division of Natural Gas Regulation (ImportExport) - E-Filing E-File Your Application Welcome to the Division of ...

  5. State Air Emission Regulations That Affect Electric Power Producers (Update) (released in AEO2006)

    Reports and Publications (EIA)

    2006-01-01

    Several states have recently enacted air emission regulations that will affect the electricity generation sector. The regulations govern emissions of NOx, SO2, CO2, and mercury from power plants.

  6. "A Regulator's Privacy Guide to Third-Party Data Access for Energy...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    "A Regulator's Privacy Guide to Third-Party Data Access for Energy Efficiency" Now Available "A Regulator's Privacy Guide to Third-Party Data Access for Energy Efficiency" Now...

  7. DOE-SPEC-3019-96; Valve-Regulated Type Lead-Acid Storage Batteries

    Office of Environmental Management (EM)

    DOE SPECIFICATION VALVE-REGULATED TYPE LEAD-ACID STORAGE BATTERIES U.S. Department of ... valve-regulated type lead-acid storage batteries, organized as follows: &21; Parts 1 through ...

  8. NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin...

    Office of Scientific and Technical Information (OSTI)

    NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin Citation Details In-Document Search Title: NMR Characterization of C3H and HCT Down-Regulated Alfalfa Lignin ...

  9. A novel synthetic derivative of melatonin, 5-hydroxy-2’-isobutyl-streptochlorin (HIS), inhibits inflammatory responses via regulation of TRIF-dependent signaling and inflammasome activation

    SciTech Connect (OSTI)

    Shim, Do-Wan; Shin, Hee Jae; Han, Ji-Won; Ji, Young-Eun; Jang, Cheol-Hun; Koppula, Sushruta; Kang, Tae-Bong; Lee, Kwang-Ho

    2015-04-15

    Melatonin is substantially reported to possess anti-inflammatory properties. In the present study, we synthesized a novel melatonin derivative, 5-hydroxy-2′-isobutyl-streptochlorin (HIS), which displayed superior anti-inflammatory properties to its parent compound. Further, we explored its underlying mechanisms in cellular and experimental animal models. Lipopolysaccharide was used to induce in vitro inflammatory responses in RAW 264.7 macrophages. LPS-primed macrophages were pulsed with biologically unrelated toxic molecules to evaluate the role of HIS on inflammasome activation. In vivo verifications were carried out using acute lung injury (ALI) and Escherichia coli-induced septic shock mouse models. HIS inhibited the production of proinflammatory mediators and cytokines such as nitric oxide, cyclooxygenase 2, IL-1β, IL-6 and TNF-α in LPS-stimulated RAW 264.7 macrophages. HIS suppressed the infiltration of immune cells into the lung and the production of pro-inflammatory cytokines such as IL-6 and TNF-α in broncho-alveolar lavage fluid in the ALI mouse model. Mechanistic studies revealed that the inhibitory effects of HIS were mediated through the regulation of the TIR domain-containing, adaptor-inducing, interferon-β (TRIF)-dependent signaling pathway from toll-like receptors. Further, HIS attenuated IL-1β secretion via the inhibition of NLRP3 inflammasome activation independent of mitochondrial ROS production. Furthermore, HIS suppressed IL-1β, IL-6 and interferon-β production in peritoneal lavage in the Escherichia coli-induced sepsis mouse model. In conclusion, HIS exerted potent anti-inflammatory effects via the regulation of TRIF-dependent signaling and inflammasome activation. Notably, the superior anti-inflammatory properties of this derivative compared with its parent compound could be a promising lead for treating various inflammatory-mediated diseases. - Highlights: • Νovel compound, 5-hydroxy-2′-isobutyl-streptochlorin (HIS) was synthesized. • HIS inhibited TRIF-dependent signaling induced by LPS stimulation. • HIS inhibited NLRP3 inflammasome activation. • HIS inhibited acute lung injury and Escherichia coli-induced septic shock.

  10. Femtosecond laser induced breakdown for combustion diagnostics

    SciTech Connect (OSTI)

    Kotzagianni, M.; Couris, S.

    2012-06-25

    The focused beam of a 100 fs, 800 nm laser is used to induce a spark in some laminar premixed air-methane flames operating with variable fuel content (equivalence ratio). The analysis of the light escaping from the plasma revealed that the Balmer hydrogen lines, H{sub {alpha}} and H{sub {beta}}, and some molecular origin emissions were the most prominent spectral features, while the CN ({Beta}{sup 2}{Sigma}{sup +}-{Chi}{sup 2}{Sigma}{sup +}) band intensity was found to depend linearly with methane content, suggesting that femtosecond laser induced breakdown spectroscopy can be a useful tool for the in-situ determination and local mapping of fuel content in hydrocarbon-air combustible mixtures.

  11. Impurity-induced moments in underdoped cuprates

    SciTech Connect (OSTI)

    Khaliullin, G.; Kilian, R.; Krivenko, S.; Fulde, P.

    1997-11-01

    We examine the effect of a nonmagnetic impurity in a two-dimensional spin liquid in the spin-gap phase, employing a drone-fermion representation of spin-1/2 operators. The properties of the local moment induced in the vicinity of the impurity are investigated and an expression for the nuclear-magnetic-resonance Knight shift is derived, which we compare with experimental results. Introducing a second impurity into the spin liquid an antiferromagnetic interaction between the moments is found when the two impurities are located on different sublattices. The presence of many impurities leads to a screening of this interaction as is shown by means of a coherent-potential approximation. Further, the Kondo screening of an impurity-induced local spin by charge carriers is discussed. {copyright} {ital 1997} {ital The American Physical Society}

  12. HIV transcription is induced in dying cells

    SciTech Connect (OSTI)

    Woloschak, G.E.; Chang-Liu, Chin-Mei; Schreck, S. |

    1995-06-01

    Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

  13. 2012 CERTS LAAR Program Peer Review - Scoping Study on Industrial Regulation - Nasr Alkadi, ORNL

    Energy Savers [EERE]

    Nasr Alkadi, Ph.D. Michael Starke, PhD Oak Ridge National Laboratory CERTS Internal Program Review FY12 DOE-CERTS Transmission Reliability R&D at Lawrence Berkeley National Laboratory. Scoping Study on Industrial Regulation September 20, 2012 2 Managed by UT-Battelle for the U.S. Department of Energy Project objective: Scoping of Regulation Reserves in Industrial Sector 2 Alcoa's Smelting Process is a significant regulation asset at Warrick, IN. * Alcoa has demonstrated Regulation Band in

  14. DOE Seeks Public Views On How Best To Streamline Existing Regulations |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Public Views On How Best To Streamline Existing Regulations DOE Seeks Public Views On How Best To Streamline Existing Regulations January 31, 2011 - 5:53pm Addthis The Department of Energy today announced two immediate steps to implement the Obama Administration's Executive Order on Improving Regulation and Regulatory Review, which directs federal agencies to review existing regulations and determine whether they are still necessary and crafted effectively to solve

  15. "Cybersecurity for State Regulators" - NARUC Primer (June 2012) |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy "Cybersecurity for State Regulators" - NARUC Primer (June 2012) "Cybersecurity for State Regulators" - NARUC Primer (June 2012) The National Association of Regulatory Utility Commissioners (NARUC) released "Cybersecurity for State Regulators," a primer that explains conceptual cybersecurity basics and points to additional resources that can help regulators develop internal cybersecurity expertise, ask questions of their utilities, engage in

  16. 40 CFR 1500-1508: CEQ Regulations for Implementing the Procedural

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Provisions of NEPA | Department of Energy 40 CFR 1500-1508: CEQ Regulations for Implementing the Procedural Provisions of NEPA 40 CFR 1500-1508: CEQ Regulations for Implementing the Procedural Provisions of NEPA PDF icon CEQ Regulations for Implementing the Procedural Provisions of NEPA (40 CFR 1500-1508) More Documents & Publications Effective Public Participation (DOE, 1998) Forty Most Asked Questions Concerning CEQ's National Environmental Policy Act Regulations (CEQ, 1986) A

  17. Guide to Federal Regulation of Sales of Imported Electricity in Canada,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Mexico and the United States | Department of Energy Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States Guide to Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States A Publication of The North American Energy Working Group on January 2005 PDF icon Guide to Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States More Documents & Publications North America: Regulation of

  18. Di(2-ethylhexyl) phthalate inhibits antral follicle growth, induces atresia, and inhibits steroid hormone production in cultured mouse antral follicles

    SciTech Connect (OSTI)

    Hannon, Patrick R. Brannick, Katherine E. Wang, Wei Gupta, Rupesh K. Flaws, Jodi A.

    2015-04-01

    Di(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental toxicant found in consumer products that causes ovarian toxicity. Antral follicles are the functional ovarian units and must undergo growth, survival from atresia, and proper regulation of steroidogenesis to ovulate and produce hormones. Previous studies have determined that DEHP inhibits antral follicle growth and decreases estradiol levels in vitro; however, the mechanism by which DEHP elicits these effects is unknown. The present study tested the hypothesis that DEHP directly alters regulators of the cell cycle, apoptosis, and steroidogenesis to inhibit antral follicle functionality. Antral follicles from adult CD-1 mice were cultured with vehicle control or DEHP (1–100 μg/ml) for 24–96 h to establish the temporal effects of DEHP on the follicle. Following 24–96 h of culture, antral follicles were subjected to gene expression analysis, and media were subjected to measurements of hormone levels. DEHP increased the mRNA levels of cyclin D2, cyclin dependent kinase 4, cyclin E1, cyclin A2, and cyclin B1 and decreased the levels of cyclin-dependent kinase inhibitor 1A prior to growth inhibition. Additionally, DEHP increased the mRNA levels of BCL2-associated agonist of cell death, BCL2-associated X protein, BCL2-related ovarian killer protein, B-cell leukemia/lymphoma 2, and Bcl2-like 10, leading to an increase in atresia. Further, DEHP decreased the levels of progesterone, androstenedione, and testosterone prior to the decrease in estradiol levels, with decreased mRNA levels of side-chain cleavage, 17α-hydroxylase-17,20-desmolase, 17β-hydroxysteroid dehydrogenase, and aromatase. Collectively, DEHP directly alters antral follicle functionality by inhibiting growth, inducing atresia, and inhibiting steroidogenesis. - Highlights: • DEHP inhibits antral follicle growth by dysregulating cell cycle regulators. • DEHP induces antral follicle atresia by dysregulating apoptosis regulators. • DEHP inhibits the production of antral follicle produced sex steroid hormones.

  19. UAP56 is an important mediator of Angiotensin II/platelet derived growth factor induced vascular smooth muscle cell DNA synthesis and proliferation

    SciTech Connect (OSTI)

    Sahni, Abha; Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 ; Wang, Nadan; Center for Translational Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107 ; Alexis, Jeffrey

    2013-02-15

    Highlights: ► Knockdown of UAP56 inhibits Angiotensin II/PDGF induced vascular smooth muscle cell proliferation. ► UAP56 is a positive regulator of E2F transcriptional activation. ► UAP56 is present in the vessel wall of low flow carotid arteries. -- Abstract: Angiotensin (Ang) II and platelet-derived growth factor (PDGF) are important mediators of pathologic vascular smooth muscle cell (VSMC) proliferation. Identifying downstream mediators of Ang II and PDGF signaling may provide insights for therapies to improve vascular proliferative diseases. We have previously demonstrated that breakpoint cluster region (Bcr) is an important mediator of Ang II/PDGF signaling in VSMC. We have recently reported that the DExD/H box protein UAP56 is an interacting partner of Bcr in regulating VSMC DNA synthesis. We hypothesized that UAP56 itself is an important regulator of VSMC proliferation. In this report we demonstrate that knockdown of UAP56 inhibits Ang II/PDGF induced VSMC DNA synthesis and proliferation, and inhibits E2F transcriptional activity. In addition, we demonstrate that UAP56 is present in the vessel wall of low-flow carotid arteries. These findings suggest that UAP56 is a regulator of VSMC proliferation and identify UAP56 as a target for preventing vascular proliferative disease.

  20. Pressure-Induced Structural Transformations of ZnO Nanowires...

    Office of Scientific and Technical Information (OSTI)

    Journal Article: Pressure-Induced Structural Transformations of ZnO Nanowires Probed by X-ray Diffraction Citation Details In-Document Search Title: Pressure-Induced Structural ...