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Sample records for venezuela regulation induced

  1. Venezuela Country Analysis Brief

    Reports and Publications (EIA)

    2015-01-01

    Venezuela is one of the world’s largest producers and exporters of crude oil. The country has been one of the largest exporters of crude oil in the Americas. As a founding member of the Organization of the Petroleum Exporting Countries (OPEC), Venezuela is an important player in the global oil market. Although oil production has declined since its peak in the late 1990s, Venezuela has been among the top exporters of crude oil to the United States have been among the largest in the world. In recent years, through significant upfront investment, an increasing share of Venezuela’s exports has been delivered to China. While Venezuela is important to the global oil market, the government’s reinvestment of oil revenues into social programs instead of reinvestment into exploration, production, and refining has led to declines in output. In 2014, Venezuela consumed 3.3 quadrillion British thermal units (Btu) of total energy.1 Oil continues to represent most of the country’s total energy consumed, and natural gas consumption has increased in the past five years. Hydroelectric power meets less than 25% of total demand, and coal represents less than 1%.

  2. LPG in Venezuela

    SciTech Connect (OSTI)

    Romero, O.

    1986-01-01

    The use of LPG for domestic consumption in Venezuela began in late 1929 when LPG was imported in lots of 500 cylinders. These cylinders were then returned to the U.S. for refilling. Total consumption at that time was some 40M/sup 3/ (250 barrels) per year and by 1937 had grown to some 540M/sup 3/ (3,400 barrels) per year. Local production of LPG from gas began in the mid thirties with a small cooling plant in the Mene Grande Field in the Lake Maracaibo area, the first field to produce oil in Venezuela (1914). This plant produced gasoline for a refinery and some of the first LPG used in Venezuela for domestic consumption. The capacity of this plant was insufficient to satisfy the growing demand for LPG which was supplied from refinery production until the development of the natural gas processing industry. At the present time, Venezuelan refineries are net consumers of LPG.

  3. Venezuela`s gas industry poised for long term growth

    SciTech Connect (OSTI)

    Croft, G.D.

    1995-06-19

    Venezuela`s enormous gas resource, combined with a new willingness to invite outside investment, could result in rapid growth in that industry into the next century. The development of liquefied natural gas exports will depend on the future course of gas prices in the US and Europe, but reserves are adequate to supply additional projects beyond the proposed Cristobal Colon project. Venezuela`s gas reserves are likely to increase if exploration for nonassociated gas is undertaken on a larger scale. The paper discusses gas reserves in Venezuela, internal gas markets, the potential for exports, competition from Trinidad, LNG export markets, and the encouragement of foreign investment in the gas industry of Venezuela.

  4. Caracas, Venezuela: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Caracas, Venezuela: Energy Resources Jump to: navigation, search Name Caracas, Venezuela Equivalent URI DBpedia GeoNames ID 3646738 Coordinates 10.5, -66.916667 Show Map...

  5. Reformulated gasoline deal with Venezuela draws heat

    SciTech Connect (OSTI)

    Begley, R.

    1994-04-06

    A fight is brewing in Congress over a deal to let Venezuela off the hook in complying with the Clean Air Act reformulated gasoline rule. When Venezuela threatened to call for a GATT panel to challenge the rule as a trade barrier, the Clinton Administration negotiated to alter the rule, a deal that members of Congress are characterizing as {open_quotes}secret{close_quotes} and {open_quotes}back door.{close_quotes}

  6. Ceuta-Tomoporo field, Venezuela

    SciTech Connect (OSTI)

    Puig, E.R.; Fernando Marcano, R. )

    1990-09-01

    Ceuta field is located in the southeastern part of the Maracaibo basin, western Venezuela. The field is a conspicuous high belonging to the Pueblo Viejo trend, a set of strike-slip faults trending northwest. The field has an area of 320 km and is divided into eight fault-bounded blocks where light- or medium-grade oil is being produced. The structural framework is characterized by a major left-slip fault and oil accumulations are associated with compressive and extensive structural features. The deposition of Eocene sediments in some areas may have been controlled by normal faulting and a period of shortening may have altered the character of some of the faults during the late Eocene or later. The main producing intervals are shallow-water marine or fluviodeltaic Miocene and Eocene sands with porosities ranging from 8 to 15% and a production potential of up to 3,500 BOPD from depths averaging 5,182 m (17,000 ft). The reservoirs seem to contain mixtures of hydrocarbons, probably due to the generation of oil in more than one oil kitchen and/or at different migration times from a common drainage area.

  7. Venezuela slates second oil field revival round

    SciTech Connect (OSTI)

    Not Available

    1992-12-07

    This paper reports that Venezuela will accept bids under a second round next year from private foreign and domestic companies for production contracts to operate marginal active as well as inactive oil fields. The first such round came earlier this year, involving about 55 other marginal, inactive fields. It resulted in two contractors signed with domestic and foreign companies. It represented the first time since nationalization of the petroleum industry in Venezuela in 1976 that private companies were allowed to produce oil in the country. A public bid tender was expected at presstime last week.

  8. Venezuela natural gas for vehicles project

    SciTech Connect (OSTI)

    Marsicobetre, D.; Molero, T.

    1998-12-31

    The Natural Gas for Vehicles (NGV) Project in Venezuela describes the development and growth of the NGV project in the country. Venezuela is a prolific oil producer with advanced exploration, production, refining and solid marketing infrastructure. Gas production is 5.2 Bscfd. The Venezuelan Government and the oil state owned company Petroleos de Venezuela (PDVSA), pursued the opportunity of using natural gas for vehicles based on the huge amounts of gas reserves present and produced every day associated with the oil production. A nationwide gas pipeline network crosses the country from south to west reaching the most important cities and serving domestic and industrial purposes but there are no facilities to process or export liquefied natural gas. NGV has been introduced gradually in Venezuela over the last eight years by PDVSA. One hundred forty-five NGV stations have been installed and another 25 are under construction. Work done comprises displacement or relocation of existing gasoline equipment, civil work, installation and commissioning of equipment. The acceptance and usage of the NGV system is reflected in the more than 17,000 vehicles that have been converted to date using the equivalent of 2,000 bbl oil/day.

  9. Plans to revive oil fields in Venezuela on track

    SciTech Connect (OSTI)

    Not Available

    1992-02-24

    This paper reports on the three operating units of Venezuela's state owned oil company Petroleos de Venezuela SA which will begin receiving bids Feb. 28 from companies interested in operating 55 inactive oil fields in nine producing areas of Venezuela. Francisco Pradas, Pdvsa executive in charge of the program, the the company expects 88 companies or combines of foreign and domestic private companies to participate in the bidding. The program, announced last year, aims to reactivate production in marginal oil fields. It will involve the first direct participation by private companies in Venezuela's oil production since nationalization in 1976.

  10. Petroleum resources of Venezuela and Trinidad and Tobago

    SciTech Connect (OSTI)

    Not Available

    1983-07-01

    The status of known and ultimately recoverable crude oil and natural gas resources of the Federal Republics of Venezuela, and Trinidad and Tobago (hereafter referred to as Trinidad) is set forth in this report. The rates that oil resources may be available to world markets are also covered in the report. A section on the petroleum geology of the region is included. The Republics of Venezuela and Trinidad share a common and ancient petroleum history. Over a century of exploration and development have resulted in the cumulative production of nearly 39 billion barrels of oil from Venezuela and over 2 billion barrels from Trinidad. Both republics have passed their peak status as oil producers. Venezuela reached its peak as the second largest producer in the world in the mid-fifties, and Trinidad attained its highest status as the eighth largest oil producer in the early forties. The report concludes that Venezuela and Trinidad have depleted slightly less than one-half of their ultimately recoverable crude oil resources. Based on feasible production rates and estimates of remaining recoverable resources, nearly two-thirds of Venezuela's oil resources and about three-fourths of Trinidad's oil resources may be depleted by the year 2000. The natural gas resources of both countries are underutilized and underdeveloped.

  11. Pros and cons of power combined cycle in Venezuela

    SciTech Connect (OSTI)

    Alvarez, C.; Hernandez, S.

    1997-09-01

    In Venezuela combined cycle power has not been economically attractive to electric utility companies, mainly due to the very low price of natural gas. Savings in cost of natural gas due to a higher efficiency, characteristic of this type of cycle, does not compensate additional investments required to close the simple cycle (heat recovery steam generator (HRSG) and steam turbine island). Low gas prices have contributed to create a situation characterized by investors` reluctance to commit capital in gas pipe lines and associated equipment. The Government is taking measures to improve economics. Recently (January 1, 1997), the Ministry of Energy and Mines raised the price of natural gas, and established a formula to tie its price to the exchange rate variation (dollar/bolivar) in an intent to stimulate investments in this sector. This is considered a good beginning after a price freeze for about three years. Another measure that has been announced is the implementation of a corporate policy of outsourcing to build new gas facilities such as pipe lines and measuring and regulation stations. Under these new circumstances, it seems that combined cycle will play an important role in the power sector. In fact, some power generation projects are considering building new plants using this technology. An economical comparative study is presented between simple and combined cycles power plant. Screening curves are showed with a gas price forecast based on the government decree recently issued, as a function of plant capacity factor.

  12. MicroRNA Regulation of Ionizing Radiation-Induced Premature Senescence

    SciTech Connect (OSTI)

    Wang Yong; Scheiber, Melissa N.; Neumann, Carola; Calin, George A.; Zhou Daohong

    2011-11-01

    Purpose: MicroRNAs (miRNAs) have emerged as critical regulators of many cellular pathways. Ionizing radiation (IR) exposure causes DNA damage and induces premature senescence. However, the role of miRNAs in IR-induced senescence has not been well defined. Thus, the purpose of this study was to identify and characterize senescence-associated miRNAs (SA-miRNAs) and to investigate the role of SA-miRNAs in IR-induced senescence. Methods and Materials: In human lung (WI-38) fibroblasts, premature senescence was induced either by IR or busulfan (BU) treatment, and replicative senescence was accomplished by serial passaging. MiRNA microarray were used to identify SA-miRNAs, and real-time reverse transcription (RT)-PCR validated the expression profiles of SA-miRNAs in various senescent cells. The role of SA-miRNAs in IR-induced senescence was characterized by knockdown of miRNA expression, using anti-miRNA oligonucleotides or by miRNA overexpression through the transfection of pre-miRNA mimics. Results: We identified eight SA-miRNAs, four of which were up-regulated (miR-152, -410, -431, and -493) and four which were down-regulated (miR-155, -20a, -25, and -15a), that are differentially expressed in both prematurely senescent (induced by IR or BU) and replicatively senescent WI-38 cells. Validation of the expression of these SA-miRNAs indicated that down-regulation of miR-155, -20a, -25, and -15a is a characteristic miRNA expression signature of cellular senescence. Functional analyses revealed that knockdown of miR-155 or miR-20a, but not miR-25 or miR-15a, markedly enhanced IR-induced senescence, whereas ectopic overexpression of miR-155 or miR-20a significantly inhibited senescence induction. Furthermore, our studies indicate that miR-155 modulates IR-induced senescence by acting downstream of the p53 and p38 mitogen-activated protein kinase (MAPK) pathways and in part via regulating tumor protein 53-induced nuclear protein 1 (TP53INP1) expression. Conclusion: Our

  13. Residential energy use and conservation in Venezuela: Results and implications of a household survey in Caracas

    SciTech Connect (OSTI)

    Figueroa, M.J.; Ketoff, A.; Masera, O.

    1992-10-01

    This document presents the final report of a study of residential energy use in Caracas, the capital of Venezuela. It contains the findings of a household energy-use survey held in Caracas in 1988 and examines options for introducing energy conservation measures in the Venezuelan residential sector. Oil exports form the backbone of the Venezuelan economy. Improving energy efficiency in Venezuela will help free domestic oil resources that can be sold to the rest of the world. Energy conservation will also contribute to a faster recovery of the economy by reducing the need for major investments in new energy facilities, allowing the Venezuelan government to direct its financial investments towards other areas of development. Local environmental benefits will constitute an important additional by-product of implementing energy-efficiency policies in Venezuela. Caracas`s residential sector shows great potential for energy conservation. The sector is characterized by high saturation levels of major appliances, inefficiency of appliances available in the market, and by careless patterns of energy use. Household energy use per capita average 6.5 GJ/per year which is higher than most cities in developing countries; most of this energy is used for cooking. Electricity accounts for 41% of all energy use, while LPG and natural gas constitute the remainder. Specific options for inducing energy conservation and energy efficiency in Caracas`s residential sector include energy-pricing policies, fuel switching, particularly from electricity to gas, improving the energy performance of new appliances and customer information. To ensure the accomplishment of an energy-efficiency strategy, a concerted effort by energy users, manufacturers, utility companies, government agencies, and research institutions will be needed.

  14. Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization

    SciTech Connect (OSTI)

    Fairman, James Wesley; Wijerathna, Sanath Ranjan; Ahmad, Md Faiz; Xu, Hai; Nakano, Ryo; Jha, Shalini; Prendergast, Jay; Welin, R. Martin; Flodin, Susanne; Roos, Annette; Nordlund, Pr; Li, Zongli; Walz, Thomas; Dealwis, Chris Godfrey

    2011-07-25

    Ribonucleotide reductase (RR) is an {alpha}{sub n}{beta}{sub n} (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the {alpha}{sub 6}-{beta}{beta}'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

  15. Structural Basis for Allosteric Regulation of Human Ribonucleotide Reductase by Nucleotide-induced Oligomerization

    SciTech Connect (OSTI)

    J Fairman; S Wijerathna; M Ahmad; H Xu; R nakano; S jha; J Prendergast; R Welin; S Flodin; et al.

    2011-12-31

    Ribonucleotide reductase (RR) is an {alpha}{sub n}{beta}{sub n} (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the {alpha}{sub 6}-{beta}{beta}'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

  16. Pedernales oilfield, eastern Venezuela: The first 100 years

    SciTech Connect (OSTI)

    Gluyas, J.; Oliver, J.; Wilson, W.

    1996-08-01

    Petroleum seeps and surface tar mats attracted oil explorers to Pedernales in eastern Venezuela 100 years ago. Commercial production from the Pedernales Field was established by Creole in 1933. In three production periods, broken by WWII and the end of the Creole-Texaco refining contract, Creole and Lagoven produced about 60 MMSTB from about 60 wells in about 60 years. Peak production was in the late 1950s, when the field delivered 12,000 BOPD. Production was stopped in 1986. In March 1993, BP Venezuela acquired the license to reactivate Pedernales on behalf of Lagoven, and BP`s first well in the field was drilled in August 1994. A second was completed in early 1995. The production from each well was sufficiently encouraging for commerciality to be declared in March 1995. Phase 1 of the field reactivation demanded a production rate of 11,500 BOPD. As of now (September, 1995) six wells, including one gas disposal well, have been completed. Wells have been placed using a combination of old well data and mapping based on a close spaced 2D seismic survey shot in early 1994. Results from these first few wells indicate that the required production rate will be achieved despite severely depleted reservoir pressures. This paper tells the story of reactivation and re-evaluation of one of eastern Venezuela`s oldest oilfields.

  17. Record of source-generated overpressures, Venezuela

    SciTech Connect (OSTI)

    Vrolijk, P.J.; Pottorf, R.J.; Maze, W.B.

    1996-12-31

    Fluid pressures affect migration of oil, gas, and water in continental margins. Burial and thermal history models describe the degree to which indercompaction or thermal expansion of fluids contribute to fluid pressure histories, but it is more difficult to evaluate how source-terms, such as oil yield or mineral dehydration reactions, impact paleo-fluid pressures. In this study, we document how a thick, maturing source rock helped create near-lithostatic fluid pressures that generated overpressures in reservoir rocks. We analyzed abundant oil-filled and rare aqueous fluid inclusions in calcite-filled fractures in the La Luna Fm. source rock and in the underlying Cogollo Gp. carbonate reservoir in the W. Maracaibo Basin, Venezuela. Homogenization temperatures (Th) of oil-filled inclusions range from 25-42{degrees}C in the La Luna Fm. and from 25-105{degrees}C in the Cogollo Gp., and associated gravities (determined from fluorescence properties) range from 28-43{degrees}API and 17-45{degrees}API, respectively. Integration of Th with the burial and thermal history of the sampled horizons leads to the conclusion that fractures in the La Luna Fm. formed under near-lithostatic fluid pressure conditions in the presence of a gas-charged oil. The values from fractures in the Cogollo Gp. are higher than in the La Luna Fm and become more variable with increasing depth below La Luna. We interpret those fractures to have formed under lower fluid pressure conditions and/or with a less gas-charged oil than for La Luna. This interpretation of the distribution of paleo-fluid pressures is supported by the observation of modern inverted fluid pressure gradients between upper and lower Cogollo Gp. reservoirs. Thus late expulsion of a gas-charged oil created near-lithostatic fluid pressures in the La Luna Fm. source rock, and those fluid pressures bled downward through fractures into the adjoining reservoir rocks, contributing to the overpressures we observe today.

  18. PKC{eta} is a negative regulator of AKT inhibiting the IGF-I induced proliferation

    SciTech Connect (OSTI)

    Shahaf, Galit; Rotem-Dai, Noa; Koifman, Gabriela; Raveh-Amit, Hadas; Frost, Sigal A.; Livneh, Etta

    2012-04-15

    The PI3K-AKT pathway is frequently activated in human cancers, including breast cancer, and its activation appears to be critical for tumor maintenance. Some malignant cells are dependent on activated AKT for their survival; tumors exhibiting elevated AKT activity show sensitivity to its inhibition, providing an Achilles heel for their treatment. Here we show that the PKC{eta} isoform is a negative regulator of the AKT signaling pathway. The IGF-I induced phosphorylation on Ser473 of AKT was inhibited by the PKC{eta}-induced expression in MCF-7 breast adenocarcinoma cancer cells. This was further confirmed in shRNA PKC{eta}-knocked-down MCF-7 cells, demonstrating elevated phosphorylation on AKT Ser473. While PKC{eta} exhibited negative regulation on AKT phosphorylation it did not alter the IGF-I induced ERK phosphorylation. However, it enhanced ERK phosphorylation when stimulated by PDGF. Moreover, its effects on IGF-I/AKT and PDGF/ERK pathways were in correlation with cell proliferation. We further show that both PKC{eta} and IGF-I confer protection against UV-induced apoptosis and cell death having additive effects. Although the protective effect of IGF-I involved activation of AKT, it was not affected by PKC{eta} expression, suggesting that PKC{eta} acts through a different route to increase cell survival. Hence, our studies show that PKC{eta} provides negative control on AKT pathway leading to reduced cell proliferation, and further suggest that its presence/absence in breast cancer cells will affect cell death, which could be of therapeutic value.

  19. Radioisotope Concentration in Lake Sediments of Maracaibo, Venezuela

    SciTech Connect (OSTI)

    Salas, A. Rangel; Viloria, T.; Sajo-Bohus, L.; Barros, H.; Greaves, E. D.; Palacios, D.

    2007-10-26

    Maracaibo Lake is one of the most important water basing and oil producing regions in Venezuela. Changes in the local environment have been monitored for chemical pollution in the past. For this study we selected a set of sediment samples collected in the shore and analyzed for its radioisotope content. Results show the gamma emitting isotopes distribution. Isotopes concentrations have been determined within the natural K, Th and U families.

  20. Venezuela recasts itself as a new frontier in the Americas

    SciTech Connect (OSTI)

    Reinsch, A.E.

    1996-09-01

    In January of this year, Venezuela captured the attention of the international energy community by welcoming back the foreign oil companies that, 20 years earlier, it had shut out of the country by nationalizing the hydrocarbon sector. The tool used to attract that attention, a new exploration bidding round, is the most publicized event staged to date in the country`s aperture process. However, it is only the latest in a series of steps taken by officials to bring international oil and gas companies back to Caracas. Venezuela`s physical attraction is easily understood. The country possesses roughly one-half of Latin America`s (including Mexico) 125 billion bbl of established, conventional crude oil reserves, plus an estimated 300 billion bbl of additional, nonconventional reserves in the ultra-heavy crude belt of the Orinoco basin. Averaging 2.8 million bpd in 1996, Venezuelan crude production represents over 35% of regional oil output. Natural gas reserves total 138 Tcf, or just over one-half of the region`s total reserves of 274 Tcfg. Annual gas output averages just under 5 Tcf, of which roughly 30% is reinjected as part of tertiary oil recovery schemes. This paper reviews the incentives, deregulation, and government policies to restore the oil and gas industry to the country.

  1. Epigenetic regulation leading to induced pluripotency drives cancer development in vivo

    SciTech Connect (OSTI)

    Ohnishi, Kotaro; Semi, Katsunori; Yamada, Yasuhiro

    2014-12-05

    Highlights: • Epigenetic regulation of failed reprogramming-associated cancer cells is discussed. • Similarity between pediatric cancer and reprogramming-associated cancer is discussed. • Concept for epigenetic cancer is discussed. - Abstract: Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by the transient expression of reprogramming factors. During the reprogramming process, somatic cells acquire the ability to undergo unlimited proliferation, which is also an important characteristic of cancer cells, while their underlying DNA sequence remains unchanged. Based on the characteristics shared between pluripotent stem cells and cancer cells, the potential involvement of the factors leading to reprogramming toward pluripotency in cancer development has been discussed. Recent in vivo reprogramming studies provided some clues to understanding the role of reprogramming-related epigenetic regulation in cancer development. It was shown that premature termination of the in vivo reprogramming result in the development of tumors that resemble pediatric cancers. Given that epigenetic modifications play a central role during reprogramming, failed reprogramming-associated cancer development may have provided a proof of concept for epigenetics-driven cancer development in vivo.

  2. IL-33 inhibits RANKL-induced osteoclast formation through the regulation of Blimp-1 and IRF-8 expression

    SciTech Connect (OSTI)

    Kiyomiya, Hiroyasu; Ariyoshi, Wataru; Okinaga, Toshinori; Kaneuji, Takeshi; Mitsugi, Sho; Sakurai, Takuma; Habu, Manabu; Yoshioka, Izumi; Tominaga, Kazuhiro; and others

    2015-05-01

    Interleukin (IL)-33 is a recently discovered proinflammatory cytokine that belongs to the IL-1 family. Several studies have reported that IL-33 inhibits osteoclast differentiation. However, the mechanism of IL-33 regulation of osteoclastogenesis remains unclear. In the present study, we examined the effect of IL-33 on osteoclast formation in vitro. IL-33 suppressed osteoclast formation in both mouse bone marrow cells and monocyte/macrophage cell line RAW264.7 cells induced by receptor activator of NF-κB ligand (RANKL) and/or macrophage stimulating factor (M-CSF). IL-33 also inhibited the expression of RANKL-induced nuclear factor of activated T-cell cytoplasmic 1 (NFATc1), thereby decreasing the expression of osteoclastogenesis-related marker genes, including Cathepsin K, Osteoclast stimulatory transmembrane protein (Oc-stamp) and Tartrate-resistant acid phosphatase (Trap). Blockage of IL-33-ST2 binding suppressed the IL-33-mediated inhibition of NFATc1. RANKL-induced B-lymphocyte-induced maturation protein-1 (Blimp-1) expression was also suppressed by IL-33, which was followed by the stimulation of anti-osteoclastic genes such as interferon regulatory factor-8 (IRF-8). These results suggest that IL-33-ST2 interactions down-regulate both RANKL-induced NFATc1 activation and osteoclast differentiation via the regulation of Blimp-1 and IRF-8 expression. - Highlights: • IL-33 inhibits RANKL-induced osteoclast formation. • IL-33 has inhibitory effect on the RANKL-induced NFATc1 expression. • IL-33-induced NFATc1 suppression depends on the regulation of Blimp-1 and IRF-8.

  3. A new approach to the oil business in Venezuela

    SciTech Connect (OSTI)

    Pradas, F.; Valdes, G. )

    1993-02-01

    Petroleos de Venezuela S.A. (PDVSA) has been meeting during the past two years with a considerable number of companies (groups) with a view to possible associations for the reactivation of known fields, joint activities in barely explored areas and the exploitation, refining, upgrading and marketing of crudes from the Orinoco Belt holder of immense reserves of extra-heavy crudes and bitumens. In Venezuela, a new approach to the oil business is under way. On January 1976, the law nationalizing the Venezuelan oil industry became effective establishing that the government retains the sole rights to the country's petroleum industry. However, Article 5 of the law made provision for associations in cases considered to be of [open quotes]National Interest.[close quotes] PDVSA became the wholly government-owned holding entity, encompassing 26 subsidiaries and affiliates. Other energy segments, particularly coal and petrochemicals, were not included in the law and since 1984 several associations with non-governmental entities have been established in Venezuela and very valuable experience has been garnered. Similarly, partnerships and acquisitions have been made in Europe and USA, mostly in refining, through PDVSA. The international petroleum industry foresees a modest, but steady increase in production during the coming decade, particularly in the second half of the nineties. This effect, added to the globalization concept, is making the traditional vertically integrated oil companies look for diverse [open quotes]lateral[close quotes] associations between groups, private and/or state owned with common interests, that will give greater security to sources of supply and market share.

  4. Venezuela No. 1 oil import source in S. America

    SciTech Connect (OSTI)

    Not Available

    1992-08-10

    This paper reports that with the exception of Venezuela, the U.S. is likely to import much oil from South American countries through 2010, the General Accounting Office reports. GAO, a congressional watchdog agency, noted the U.S. imports about 4% of its oil from Colombia, Ecuador, and Trinidad and Tobago and possibly could import from Argentina, Bolivia, Brazil, Chile, and Peru in the future. It the the eight countries' crude oil reserves are expected to increase about 30% by 2000, then slide about 2% by 2010. Their oil production is expected to climb about 21% over 1990 by 2000, then level off until 2010.

  5. Geology and geochemistry of crude oils, Bolivar coastal fields, Venezuela

    SciTech Connect (OSTI)

    Bockmeulen, H.; Barker, C.; Dickey, P.A.

    1983-02-01

    The Bolivar Coastal Fields (BCF) are located on the eastern margin of Lake Maracaibo, Venezuela. They form the largest oil field outside of the Middle East and contain mostly heavy oil with a gravity less than 22/sup 0/ API. Thirty crude oils from the BCF were collected along two parallel and generally southwest-northeast trends. These oils were characterized by their API gravity, percent saturates, aromatics, NSO and asphalitic compounds, gas chromatograms for whole oils, C/sub 4/-C/sub 7/ fractions, and aromatics. Also, 24 associated waters were sampled and analyzed for Ca/sup + +/, Mg/sup + +/, Na/sup +/, HCO/sub 3//sup -/, CO/sub 3//sup - -/, SO/sub 4//sup - -/, pH, and total dissolved solids (TDS). The geological and geochemical significances of these analyses are discussed with particular emphasis on the genesis of the petroleum.

  6. Potential options to reduce GHG emissions in Venezuela

    SciTech Connect (OSTI)

    Pereira, N.; Bonduki, Y.; Perdomo, M.

    1996-12-31

    The Government of Venezuela ratified the United Nations Framework Convention on Climate Change (UNFCCC) in December, 1994. The Convention requires all parties to develop and publish national inventories of anthropogenic greenhouse gas emissions (GHG) as well as national plans to reduce or control emissions, taking into account their common but differentiated responsibilities and their specific national and regional development priorities, objectives, and circumstances. Within this context, the Ministry of Environment and Renewable Natural Resources and the Ministry of Energy and Mines developed the `Venezuelan Case-Study to Address Climate Change`. The study was initiated in October 1993, with the financial and technical assistance of the Government of United States, through the U.S. Country Studies Program (USCSP), and the Global Environment Facility (GEF), through the United Nations Environment Programme (UNEP).

  7. Venezuela bets on heavy crude for long term

    SciTech Connect (OSTI)

    Abraham, K.S.

    1997-01-01

    In the heart of eastern Venezuela lies the Orinoco Belt, a vast reserve of heavy crudes and bitumen that equate to only 8{degree} to 10{degree} API. At the beginning of the 1920s, a number of foreign companies explored this area. However, they realized that this crude was too heavy to be produced commercially and abandoned their exploratory sites. In 1978--1980, state firm PDVSA made a large effort to quantify the resources. Geologists finally estimated the in-place reserves at 1.2 trillion bbl, of which 267 billion bbl (41 billion t) were considered recoverable. If produced at a rate of 1.5 million bopd, these reserves would last nearly 500 years. PDVSA experimented with various methods to produce the bitumen. Finally, in the mid-1980s, a breakthrough of sorts was achieved, almost by accident. Lab technicians discovered that bitumen will continue to burn effectively when emulsified with water. Company officials describe the flame as resembling burning gas. This discovery began the rapidly accelerating process to develop what is called the now-patented Orimulsion production. PDVSA managers discarded their plans to supply refineries with bitumen and adopted a new strategy of targeting Orimulsion as an alternative boiler fuel for electric power-generating stations. To oversee this project, a new subsidiary, Bitor (a compressed combination of the terms, bitumen and Orinoco), was created. Bitor operations are described.

  8. A modern look at the petroleum geology of the Maracaibo basin, Venezuela

    SciTech Connect (OSTI)

    Stauffer, K.W.; Croft, G.D.

    1995-06-05

    The Maracaibo basin of western Venezuela is one of the world`s most important oil producing basins, with a cumulative production of more than 35 billion bbl. The reasons for this great wealth of hydrocarbons are a combination of source beds of excellent quality, thick reservoirs with high porosity and permeability, and a series of sealing shales, faults, and unconformities, which provide large and numerous traps. Recent discoveries combined with Venezuela`s opening to international investment suggest that the story of this basin is far from over. Surprisingly little exploration has taken place in large parts of the basin, especially southwest of Lake Maracaibo and in the southern part of the lake. This paper describes the history of the basin, stratigraphy, structure, oil fields, and its future prospects.

  9. Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling

    SciTech Connect (OSTI)

    Liu, Chang; Chen, Lin; Zeng, Jing; Cui, Jian; Ning, Jiao-nin; Wang, Guan-song; Belguise, Karine; Wang, Xiaobo; Qian, Gui-sheng; Lu, Kai-zhi; Yi, Bin

    2015-08-01

    Hepatopulmonary syndrome (HPS) is characterized by an arterial oxygenation defect induced by intrapulmonary vasodilation (IPVD) that increases morbidity and mortality. In our previous study, it was determined that both the proliferation and the myogenic differentiation of pulmonary microvascular endothelial cells (PMVECs) play a key role in the development of IPVD. However, the molecular mechanism underlying the relationship between IPVD and the myogenic differentiation of PMVECs remains unknown. Additionally, it has been shown that bone morphogenic protein-2 (BMP2), via the control of protein expression, may regulate cell differentiation including cardiomyocyte differentiation, neuronal differentiation and odontoblastic differentiation. In this study, we observed that common bile duct ligation (CBDL)-rat serum induced the upregulation of the expression of several myogenic proteins (SM-α-actin, calponin, SM-MHC) and enhanced the expression levels of BMP2 mRNA and protein in PMVECs. We also observed that both the expression levels of Smad1/5 and the activation of phosphorylated Smad1/5 were significantly elevated in PMVECs following exposure to CBDL-rat serum, which was accompanied by the down-regulation of Smurf1. The blockage of the BMP2/Smad signaling pathway with Noggin inhibited the myogenic differentiation of PMVECs, a process that was associated with relatively low expression levels of both SM-α-actin and calponin in the setting of CBDL-rat serum exposure, although SM-MHC expression was not affected. These findings suggested that the BMP2/Smad signaling pathway is involved in the myogenic differentiation of the PMVECs. In conclusion, our data highlight the pivotal role of BMP2 in the CBDL-rat serum-induced myogenic differentiation of PMVECs via the activation of both Smad1 and Smad5 and the down-regulation of Smurf1, which may represent a potential therapy for HPS-induced pulmonary vascular remodeling. - Highlights: • CBDL-rat serum promotes the myogenic

  10. Coal bed methane potential in Venezuela-The forgotten resource

    SciTech Connect (OSTI)

    Vasquez-Herrera, A.R.; Bereskin, S.R.; McLennan, J.D.

    1996-08-01

    In nations already possessing riches of hydrocarbons situated in conventional reservoirs, evaluation of coal-bearing sequences for potential gas is logically delayed or ignored. Nonetheless, Venezuelan coals have long been recognized as stratigraphically associated with oil accumulations, but because coalbed methane (CBM) is a relatively new worldwide phenomenon, CBM potential has not been widely assessed in the country. Two general areas contain vast accumulations of coal for potential CBM activity: (1) the Maracaibo basin, containing the Guasare (northwest), Lobatera-Santo Domingo (southwest) and Urumaco (northeast) districts; and (2) the Oficina basin in eastern Venezuela possessing abundant accumulations related to the Faja Petrolifera de Orinoco (Orinoco Oil Belt). In both basins, high volatile bituminous and lignitic coals of mostly Oligo-Miocene age are abundantly found. Older coals are also present especially in the Maracaibo area. Two factors represent powerful incentives for CBM exploitation: addition of known reserves for economic considerations, and aid in bringing heavy crude oil to the surface by additional gas lift and oil viscosity reduction. Other favorable factors important for CBM methodology include: (1) abundant coals lying above known conventional reservoir targets; (2).6 - 1% vitrinite reflectance measurements in the Orinoco Oil Belt; (3) many coals occurring above 1500 m; (4) documented mine explosions especially in the 1920s and 1930s; (5) a strong tectonic overprint to perhaps add shear fractures to already cleated coals; (6) individual coal thickness up to 12 m with averages in the .8 m range; and (7) gas shows while drilling coal-rich intervals.

  11. Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells

    SciTech Connect (OSTI)

    Yang, Bin; Li, Wei; Zheng, Qichang; Qin, Tao; Wang, Kun; Li, Jinjin; Guo, Bing; Yu, Qihong; Wu, Yuzhe; Gao, Yang; Cheng, Xiang; Hu, Shaobo; Kumar, Stanley Naveen; Liu, Sanguang; Song, Zifang

    2015-07-17

    Stromal-derived Factor-1 (SDF-1) derived from vascular smooth muscle cells (VSMCs) contributes to vascular repair and remodeling in various vascular diseases. In this study, the mechanism underlying regulation of SDF-1 expression by interleukin-1α (IL-1α) was investigated in primary rat VSMCs. We found IL-1α promotes SDF-1 expression by up-regulating CCAAT-enhancer-binding protein β (C/EBPβ) in an IκB kinase β (IKKβ) signaling-dependent manner. Moreover, IL-1α-induced expression of C/EBPβ and SDF-1 was significantly potentiated by knockdown of transforming growth factor β-activated kinase 1 (TAK1), an upstream activator of IKKβ signaling. In addition, we also demonstrated that TAK1/p38 mitogen-activated protein kinase (p38 MAPK) signaling exerted negative effect on IL-1α-induced expression of C/EBPβ and SDF-1 through counteracting ROS-dependent up-regulation of nuclear factor erythroid 2-related factor 2 (NRF2). In conclusion, TAK1 acts as an important regulator of IL-1α-induced SDF-1 expression in VSMCs, and modulating activity of TAK1 may serve as a potential strategy for modulating vascular repair and remodeling. - Highlights: • IL-1α induces IKKβ signaling-dependent SDF-1 expression by up-regulating C/EBPβ. • Activation of TAK1 by IL-1α negatively regulates C/EBPβ-dependent SDF-1 expression. • IL-1α-induced TAK1/p38 MAPK signaling counteracts ROS-dependent SDF-1 expression. • TAK1 counteracts IL-1α-induced SDF-1 expression by attenuating NRF2 up-regulation.

  12. Five Years of Cyclotron Radioisotope Production Experiences at the First PET-CT in Venezuela

    SciTech Connect (OSTI)

    Colmenter, L.; Coelho, D.; Esteves, L. M.; Ruiz, N.; Morales, L.; Lugo, I.; Sajo-Bohus, L.; Liendo, J. A.; Greaves, E. D.; Barros, H.; Castillo, J.

    2007-10-26

    Five years operation of a compact cyclotron installed at PET-CT facility in Caracas, Venezuela is given. Production rate of {sup 18}F labeled FDG, operation and radiation monitoring experience are included. We conclude that {sup 18}FDG CT-PET is the most effective technique for patient diagnosis.

  13. Household energy use in urban Venezuela: Implications from surveys in Maracaibo, Valencia, Merida, and Barcelona-Puerto La Cruz

    SciTech Connect (OSTI)

    Figueroa, M.J.; Sathaye, J.

    1993-08-01

    This report identifies the most important results of a comparative analysis of household commercial energy use in Venezuelan urban cities. The use of modern fuels is widespread among all cities. Cooking consumes the largest share of urban household energy use. The survey documents no use of biomass and a negligible use of kerosene for cooking. LPG, natural gas, and kerosene are the main fuels available. LPG is the fuel choice of low-income households in all cities except Maracaibo, where 40% of all households use natural gas. Electricity consumption in Venezuela`s urban households is remarkably high compared with the levels used in households in comparable Latin American countries and in households of industrialized nations which confront harsher climatic conditions and, therefore, use electricity for water and space heating. The penetration of appliances in Venezuela`s urban households is very high. The appliances available on the market are inefficient, and there are inefficient patterns of energy use among the population. Climate conditions and the urban built form all play important roles in determining the high level of energy consumption in Venezuelan urban households. It is important to acknowledge the opportunities for introducing energy efficiency and conservation in Venezuela`s residential sector, particularly given current economic and financial constraints, which may hamper the future provision of energy services.

  14. Alisol B 23-acetate protects against ANIT-induced hepatotoxity and cholestasis, due to FXR-mediated regulation of transporters and enzymes involved in bile acid homeostasis

    SciTech Connect (OSTI)

    Meng, Qiang; Chen, Xin-li; Wang, Chang-yuan; Liu, Qi; Sun, Hui-jun; Sun, Peng-yuan; Huo, Xiao-kui; Liu, Zhi-hao; Yao, Ji-hong; Liu, Ke-xin

    2015-03-15

    Intrahepatic cholestasis is a clinical syndrome with systemic and intrahepatic accumulation of excessive toxic bile acids that ultimately cause hepatobiliary injury. Appropriate regulation of bile acids in hepatocytes is critically important for protection against liver injury. In the present study, we characterized the protective effect of alisol B 23-acetate (AB23A), a natural triterpenoid, on alpha-naphthylisothiocyanate (ANIT)-induced liver injury and intrahepatic cholestasis in mice and further elucidated the mechanisms in vivo and in vitro. AB23A treatment dose-dependently protected against liver injury induced by ANIT through reducing hepatic uptake and increasing efflux of bile acid via down-regulation of hepatic uptake transporters (Ntcp) and up-regulation of efflux transporter (Bsep, Mrp2 and Mdr2) expression. Furthermore, AB23A reduced bile acid synthesis through repressing Cyp7a1 and Cyp8b1, increased bile acid conjugation through inducing Bal, Baat and bile acid metabolism through an induction in gene expression of Sult2a1. We further demonstrate the involvement of farnesoid X receptor (FXR) in the hepatoprotective effect of AB23A. The changes in transporters and enzymes, as well as ameliorative liver histology in AB23A-treated mice were abrogated by FXR antagonist guggulsterone in vivo. In vitro evidences also directly demonstrated the effect of AB23A on FXR activation in a dose-dependent manner using luciferase reporter assay in HepG2 cells. In conclusion, AB23A produces protective effect against ANIT-induced hepatotoxity and cholestasis, due to FXR-mediated regulation of transporters and enzymes. - Highlights: • AB23A has at least three roles in protection against ANIT-induced liver injury. • AB23A decreases Ntcp, and increases Bsep, Mrp2 and Mdr2 expression. • AB23A represses Cyp7a1 and Cyp8b1 through inducing Shp and Fgf15 expression. • AB23A increases bile acid metabolism through inducing Sult2a1 expression. • FXR activation is involved

  15. Regulation of ozone-induced lung inflammation and injury by the β-galactoside-binding lectin galectin-3

    SciTech Connect (OSTI)

    Sunil, Vasanthi R.; Francis, Mary; Vayas, Kinal N.; Cervelli, Jessica A.; Choi, Hyejeong; Laskin, Jeffrey D.; Laskin, Debra L.

    2015-04-15

    Macrophages play a dual role in ozone toxicity, contributing to both pro- and anti-inflammatory processes. Galectin-3 (Gal-3) is a lectin known to regulate macrophage activity. Herein, we analyzed the role of Gal-3 in the response of lung macrophages to ozone. Bronchoalveolar lavage (BAL) and lung tissue were collected 24–72 h after exposure (3 h) of WT and Gal-3{sup -/-} mice to air or 0.8 ppm ozone. In WT mice, ozone inhalation resulted in increased numbers of proinflammatory (Gal-3{sup +}, iNOS{sup +}) and anti-inflammatory (MR-1{sup +}) macrophages in the lungs. While accumulation of iNOS{sup +} macrophages was attenuated in Gal-3{sup -/-} mice, increased numbers of enlarged MR-1{sup +} macrophages were noted. This correlated with increased numbers of macrophages in BAL. Flow cytometric analysis showed that these cells were CD11b{sup +} and consisted mainly (> 97%) of mature (F4/80{sup +}CD11c{sup +}) proinflammatory (Ly6GLy6C{sup hi}) and anti-inflammatory (Ly6GLy6C{sup lo}) macrophages. Increases in both macrophage subpopulations were observed following ozone inhalation. Loss of Gal-3 resulted in a decrease in Ly6C{sup hi} macrophages, with no effect on Ly6C{sup lo} macrophages. CD11b{sup +}Ly6G{sup +}Ly6C{sup +} granulocytic (G) and monocytic (M) myeloid derived suppressor cells (MDSC) were also identified in the lung after ozone. In Gal-3{sup -/-} mice, the response of G-MDSC to ozone was attenuated, while the response of M-MDSC was heightened. Changes in inflammatory cell populations in the lung of ozone treated Gal-3{sup -/-} mice were correlated with reduced tissue injury as measured by cytochrome b5 expression. These data demonstrate that Gal-3 plays a role in promoting proinflammatory macrophage accumulation and toxicity in the lung following ozone exposure. - Highlights: • Multiple monocytic-macrophage subpopulations accumulate in the lung after ozone inhalation. • Galectin-3 plays a proinflammatory role in ozone-induced lung injury. • In the

  16. Norisoboldine ameliorates collagen-induced arthritis through regulating the balance between Th17 and regulatory T cells in gut-associated lymphoid tissues

    SciTech Connect (OSTI)

    Tong, Bei; Dou, Yannong; Wang, Ting; Yu, Juntao; Wu, Xin; Lu, Qian; Chou, Guixin; Wang, Zhengtao; Kong, Lingyi; Dai, Yue; Xia, Yufeng

    2015-01-01

    Norisoboldine (NOR), the main active ingredient of the dry root of Lindera aggregata, was previously proven to have substantial therapeutic effects on collagen-induced arthritis (CIA) in mice by oral administration. However, it exhibited a very poor bioavailability in normal rats. The pharmacokinetic–pharmacodynamics disconnection attracts us to explore its anti-arthritic mechanism in more detail. In this study, NOR, administered orally, markedly attenuated the pathological changes in CIA rats, which was accompanied by the down-regulation of pro-inflammatory cytokines and the up-regulation of anti-inflammatory cytokine IL-10. Pharmacokinetic studies demonstrated that the plasma concentration of NOR was moderately elevated in CIA rats compared with normal rats, but it was still far lower than the minimal effective concentration required for inhibiting the proliferation and activation of T lymphocytes in vitro. Interestingly, NOR was shown to regulate the balance between Th17 and regulatory T (Treg) cells in the intestinal lymph nodes more strikingly than in other tissues. It could increase the expression of Foxp3 mRNA in both gut and joints, and markedly up-regulate the number of integrin α4β7 (a marker of gut source)-positive Foxp3{sup +} cells in the joints of CIA rats. These results suggest that the gut might be the primary action site of NOR, and NOR exerts anti-arthritis effect through regulating the balance between Th17 and Treg cells in intestinal lymph nodes and yielding a trafficking of lymphocytes (especially Treg cells) from the gut to joint. The findings of the present study also provide a plausible explanation for the anti-arthritic effects of poorly absorbed compounds like NOR. - Highlights: • Norisoboldine, administered orally, markedly attenuates the clinical signs of CIA. • Norisoboldine regulates the balance of Th17/Treg cells in the intestinal lymph node. • Norisoboldine induces the migration of Treg cells from the gut to joint.

  17. Ginsenoside-Rg{sub 1} induces angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a

    SciTech Connect (OSTI)

    Kwok, Hoi-Hin; Chan, Lai-Sheung; Poon, Po-Ying; Yue, Patrick Ying-Kit; Wong, Ricky Ngok-Shun

    2015-09-15

    Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. Ginsenoside-Rg{sub 1} (Rg{sub 1}), one of the most abundant active components of ginseng, has been demonstrated as an angiogenesis-stimulating compound in different models. There is increasing evidence implicating microRNAs (miRNAs), a group of non-coding RNAs, as important regulators of angiogenesis, but the role of microRNAs in Rg{sub 1}-induced angiogenesis has not been fully explored. In this report, we found that stimulating endothelial cells with Rg{sub 1} could reduce miR-23a expression. In silico experiments predicted hepatocyte growth factor receptor (MET), a well-established mediator of angiogenesis, as the target of miR-23a. Transfection of the miR-23a precursor or inhibitor oligonucleotides validated the inverse relationship of miR-23a and MET expression. Luciferase reporter assays further confirmed the interaction between miR-23a and the MET mRNA 3′-UTR. Intriguingly, ginsenoside-Rg{sub 1} was found to increase MET protein expression in a time-dependent manner. We further demonstrated that ginsenoside-Rg{sub 1}-induced angiogenic activities were indeed mediated through the down-regulation of miR-23a and subsequent up-regulation of MET protein expression, as confirmed by gain- and loss-of-function angiogenic experiments. In summary, our results demonstrated that ginsenoside-Rg{sub 1} could induce angiogenesis by the inverse regulation of MET tyrosine kinase receptor expression through miR-23a. This study has broadened our understanding of the non-genomic effects of ginsenoside-Rg{sub 1,} and provided molecular evidence that warrant further development of natural compound as novel angiogenesis-promoting therapy. - Highlights: • Therapeutic angiogenesis has been implicated in ischemic diseases and wound healing. • Ginsenoside-Rg{sub 1} (Rg{sub 1}) has been demonstrated as an angiogenesis-stimulating compound. • We found that Rg{sub 1} induces angiogenesis by

  18. JBP485 improves gentamicin-induced acute renal failure by regulating the expression and function of Oat1 and Oat3 in rats

    SciTech Connect (OSTI)

    Guo, Xinjin; Meng, Qiang; Liu, Qi; Wang, Changyuan; Sun, Huijun; Peng, Jinyong; Ma, Xiaochi; Kaku, Taiichi; Liu, Kexin

    2013-09-01

    We investigated the effects of JBP485 (an anti-inflammatory dipeptide and a substrate of OAT) on regulation of the expression and function of renal Oat1 and Oat3, which can accelerate the excretion of accumulated uremic toxins (e.g. indoxyl sulfate) in the kidney to improve gentamicin-induced ARF in rats. JBP485 caused a significant decrease in the accumulation of endogenous substances (creatinine, blood urea nitrogen and indoxyl sulfate) in vivo, an increase in the excretion of exogenous compounds (lisinopril and inulin) into urine, and up-regulation of the expressions of renal Oat1 and Oat3 in the kidney tissues and slices via substrate induction. To determine the effect of JBP485 on the accelerated excretion of uremic toxins mediated by Oat1 and Oat3, the mRNA and protein expression levels of renal basolateral Oats were assessed by quantitative real-time PCR, western blot, immunohistochemical analysis and an immunofluorescence method. Gentamicin down-regulated the expression of Oats mRNA and protein in rat kidney, and these effects were reversed after administration of JBP485. In addition, JBP485 caused a significant decrease in MPO and MDA levels in the kidney, and improved the pathological condition of rat kidney. These results indicated that JBP485 improved acute renal failure by increasing the expression and function of Oat1 and Oat3, and by decreasing overoxidation of the kidney in gentamicin-induced ARF rats. - Highlights: • JBP485 could up-regulate function and expression of Oat1 and Oat3 in kidney. • Effects of JBP485 on ARF are mediated by stimulating excretion of uremic toxins. • JBP485 protected against gentamicin-induced ARF by decreasing MPO and MDA.

  19. Moderate extracellular acidification inhibits capsaicin-induced cell death through regulating calcium mobilization, NF-{kappa}B translocation and ROS production in synoviocytes

    SciTech Connect (OSTI)

    Hu, Fen; Yang, Shuang; Zhao, Dan; Zhu, Shuyan; Wang, Yuxiang; Li, Junying

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Moderate extracellular acidification regulates intracellular Ca{sup 2+} mobilization. Black-Right-Pointing-Pointer Moderate acidification activates NF-{kappa}B nuclear translocation in synoviocytes. Black-Right-Pointing-Pointer Moderate acidification depresses the ROS production induced by capsaicin. Black-Right-Pointing-Pointer Moderate acidification inhibits capsaicin-caused synoviocyte death. -- Abstract: We previously show the expression of transient receptor potential vanilloid 1 (TRPV1) in primary synoviocytes from collagen-induced arthritis (CIA) rats. Capsaicin and lowered extracellular pH from 7.4 to 5.5 induce cell death through TRPV1-mediated Ca{sup 2+} entry and reactive oxygen species (ROS) production. However, under the pathological condition in rheumatoid arthritis, the synovial fluid is acidified to a moderate level (about pH 6.8). In the present study, we examined the effects of pH 6.8 on the TRPV1-mediated cell death. Our finding is different or even opposite from what was observed at pH 5.5. We found that the moderate extracellular acidification (from pH 7.4 to 6.8) inhibited the capsaicin-induced Ca{sup 2+} entry through attenuating the activity of TRPV1. In the mean time, it triggered a phospholipse C (PLC)-related Ca{sup 2+} release from intracellular stores. The nuclear translocation of NF-{kappa}B was found at pH 6.8, and this also depends on PLC activation. Moreover, the capsaicin-evoked massive ROS production and cell death were depressed at pH 6.8, both of which are dependent on the activation of PLC and NF-{kappa}B. Taken together, these results suggested that the moderate extracellular acidification inhibited the capsaicin-induced synoviocyte death through regulating Ca{sup 2+} mobilization, activating NF-{kappa}B nuclear translocation and depressing ROS production.

  20. Production optimization of sucker rod pumping wells producing viscous oil in Boscan field, Venezuela

    SciTech Connect (OSTI)

    Guirados, C.; Sandoval, J.; Rivas, O.; Troconis, H.

    1995-12-31

    Boscan field is located in the western coast of Maracaibo lake and is operated by Maraven S.A., affiliate of Petroleos de Venezuela S.A. It has 315 active wells, 252 of which are produced with sucker rod pumping. Other artificial lift methods currently applied in this field are hydraulic (piston) pumping (39 wells) and ESP (24 wells). This paper presents the results of the production optimization of two sucker rod pumping wells of Boscan field producing viscous oil. This optimization has been possible due to the development of a new production scheme and the application of system analysis in completion design. The new production scheme involves the utilization of a subsurface stuffing box assembly and a slotted housing, both designed and patented by Intevep S.A., affiliate of Petroleos de Venezuela S.A. The completion design method and software used in the optimization study were also developed by Intevep S.A. The new production scheme and design method proved to be effective in preventing the causes of the above mentioned problems, allowing the increase of oil production under better operating conditions.

  1. Copper deficiency alters cell bioenergetics and induces mitochondrial fusion through up-regulation of MFN2 and OPA1 in erythropoietic cells

    SciTech Connect (OSTI)

    Bustos, Rodrigo I.; Jensen, Erik L.; Ruiz, Lina M.; Rivera, Salvador; Ruiz, Sebastin; Simon, Felipe; Riedel, Claudia; Millennium Institute of Immunology and Immunotherapy, Santiago ; Ferrick, David; Elorza, Alvaro A.

    2013-08-02

    Highlights: In copper deficiency, cell proliferation is not affected. In turn, cell differentiation is impaired. Enlarged mitochondria are due to up-regulation of MNF2 and OPA1. Mitochondria turn off respiratory chain and ROS production. Energy metabolism switch from mitochondria to glycolysis. -- Abstract: Copper is essential in cell physiology, participating in numerous enzyme reactions. In mitochondria, copper is a cofactor for respiratory complex IV, the cytochrome c oxidase. Low copper content is associated with anemia and the appearance of enlarged mitochondria in erythropoietic cells. These findings suggest a connection between copper metabolism and bioenergetics, mitochondrial dynamics and erythropoiesis, which has not been explored so far. Here, we describe that bathocuproine disulfonate-induced copper deficiency does not alter erythropoietic cell proliferation nor induce apoptosis. However it does impair erythroid differentiation, which is associated with a metabolic switch between the two main energy-generating pathways. That is, from mitochondrial function to glycolysis. Switching off mitochondria implies a reduction in oxygen consumption and ROS generation along with an increase in mitochondrial membrane potential. Mitochondrial fusion proteins MFN2 and OPA1 were up-regulated along with the ability of mitochondria to fuse. Morphometric analysis of mitochondria did not show changes in total mitochondrial biomass but rather bigger mitochondria because of increased fusion. Similar results were also obtained with human CD34+, which were induced to differentiate into red blood cells. In all, we have shown that adequate copper levels are important for maintaining proper mitochondrial function and for erythroid differentiation where the energy metabolic switch plus the up-regulation of fusion proteins define an adaptive response to copper deprivation to keep cells alive.

  2. Aspirin-triggered resolvin D1 down-regulates inflammatory responses and protects against endotoxin-induced acute kidney injury

    SciTech Connect (OSTI)

    Chen, Jiao; Shetty, Sreerama; Zhang, Ping; Gao, Rong; Hu, Yuxin; Wang, Shuxia; Li, Zhenyu; Fu, Jian

    2014-06-01

    The presence of endotoxin in blood can lead to acute kidney injury (AKI) and septic shock. Resolvins, the endogenous lipid mediators derived from docosahexaenoic acid, have been reported to exhibit potent anti-inflammatory action. Using a mouse model of lipopolysaccharide (LPS)-induced AKI, we investigated the effects of aspirin-triggered resolvin D1 (AT-RvD1) on inflammatory kidney injury. Administration of AT-RvD1 1 h after LPS challenge protected the mice from kidney injury as indicated by the measurements of blood urea nitrogen, serum creatinine, and morphological alterations associated with tubular damage. The protective effects were evidenced by decreased neutrophil infiltration in the kidney indicating reduction in inflammation. AT-RvD1 treatment restored kidney cell junction protein claudin-4 expression, which was otherwise reduced after LPS challenge. AT-RvD1 treatment inhibited endotoxin-induced NF-κB activation and suppressed LPS-induced ICAM-1 and VCAM-1 expression in the kidney. Moreover, AT-RvD1 treatment markedly decreased LPS-induced IL-6 level in the kidney and blocked IL-6-mediated signaling including STAT3 and ERK phosphorylation. Our findings demonstrate that AT-RvD1 is a potent anti-inflammatory mediator in LPS-induced kidney injury, and AT-RvD1 has therapeutic potential against AKI during endotoxemia.

  3. Lipid rafts regulate PCB153-induced disruption of occludin and brain endothelial barrier function through protein phosphatase 2A and matrix metalloproteinase-2

    SciTech Connect (OSTI)

    Eum, Sung Yong Jaraki, Dima; András, Ibolya E.; Toborek, Michal

    2015-09-15

    Occludin is an essential integral transmembrane protein regulating tight junction (TJ) integrity in brain endothelial cells. Phosphorylation of occludin is associated with its localization to TJ sites and incorporation into intact TJ assembly. The present study is focused on the role of lipid rafts in polychlorinated biphenyl (PCB)-induced disruption of occludin and endothelial barrier function. Exposure of human brain endothelial cells to 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB153) induced dephosphorylation of threonine residues of occludin and displacement of occludin from detergent-resistant membrane (DRM)/lipid raft fractions within 1 h. Moreover, lipid rafts modulated the reduction of occludin level through activation of matrix metalloproteinase 2 (MMP-2) after 24 h PCB153 treatment. Inhibition of protein phosphatase 2A (PP2A) activity by okadaic acid or fostriecin markedly protected against PCB153-induced displacement of occludin and increased permeability of endothelial cells. The implication of lipid rafts and PP2A signaling in these processes was further defined by co-immunoprecipitation of occludin with PP2A and caveolin-1, a marker protein of lipid rafts. Indeed, a significant MMP-2 activity was observed in lipid rafts and was increased by exposure to PCB153. The pretreatment of MMP-2 inhibitors protected against PCB153-induced loss of occludin and disruption of lipid raft structure prevented the increase of endothelial permeability. Overall, these results indicate that lipid raft-associated processes, such as PP2A and MMP-2 activation, participate in PCB153-induced disruption of occludin function in brain endothelial barrier. This study contributes to a better understanding of the mechanisms leading to brain endothelial barrier dysfunction in response to exposure to environmental pollutants, such as ortho-substituted PCBs. - Highlights: • PCB153 disturbed human brain endothelial barrier through disruption of occludin. • Lipid raft-associated PP

  4. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  5. EGCG protects endothelial cells against PCB 126-induced inflammation through inhibition of AhR and induction of Nrf2-regulated genes

    SciTech Connect (OSTI)

    Han, Sung Gu; Department of Animal and Food Sciences, College of Agriculture, University of Kentucky, Lexington, KY 40536 ; Han, Seong-Su; Toborek, Michal; Hennig, Bernhard; Department of Animal and Food Sciences, College of Agriculture, University of Kentucky, Lexington, KY 40536

    2012-06-01

    Tea flavonoids such as epigallocatechin gallate (EGCG) protect against vascular diseases such as atherosclerosis via their antioxidant and anti-inflammatory functions. Persistent and widespread environmental pollutants, including polychlorinated biphenyls (PCB), can induce oxidative stress and inflammation in vascular endothelial cells. Even though PCBs are no longer produced, they are still detected in human blood and tissues and thus considered a risk for vascular dysfunction. We hypothesized that EGCG can protect endothelial cells against PCB-induced cell damage via its antioxidant and anti-inflammatory properties. To test this hypothesis, primary vascular endothelial cells were pretreated with EGCG, followed by exposure to the coplanar PCB 126. Exposure to PCB 126 significantly increased cytochrome P450 1A1 (Cyp1A1) mRNA and protein expression and superoxide production, events which were significantly attenuated following pretreatment with EGCG. Similarly, EGCG also reduced DNA binding of NF-κB and downstream expression of inflammatory markers such as monocyte chemotactic protein-1 (MCP-1) and vascular cell adhesion protein-1 (VCAM-1) after PCB exposure. Furthermore, EGCG decreased endogenous or base-line levels of Cyp1A1, MCP-1 and VCAM-1 in endothelial cells. Most of all, treatment of EGCG upregulated expression of NF-E2-related factor 2 (Nrf2)-controlled antioxidant genes, including glutathione S transferase (GST) and NAD(P)H:quinone oxidoreductase 1 (NQO1), in a dose-dependent manner. In contrast, silencing of Nrf2 increased Cyp1A1, MCP-1 and VCAM-1 and decreased GST and NQO1 expression, respectively. These data suggest that EGCG can inhibit AhR regulated genes and induce Nrf2-regulated antioxidant enzymes, thus providing protection against PCB-induced inflammatory responses in endothelial cells. -- Highlights: ► PCBs cause endothelial inflammation and subsequent atherosclerosis. ► Nutrition can modulate toxicity by environmental pollutants. ► We

  6. Petroleum-processing projects in Venezuela: Definitional-Mission report. Export trade information

    SciTech Connect (OSTI)

    Grady, R.B.; Shrivastava, V.K.

    1992-02-01

    The U.S. Trade and Development Program (TDP) contracted a Definitional Mission to evaluate the prospects of TDP funding feasibility studies of three petroleum refining projects for Maraven, an integrated oil subsidiary of Petroleos de Venezuela, S.A. (PDVSA). Maraven has requested TDP support for feasibility studies of the following projects: Zuata (Orinoco Belt) Extra Heavy Crude Upgrading, Boscan Heavy Crude Gasification Complex, and High Quality Lube Oil Base Manufacting Project. The ratio of U.S. export potential to the cost of the feasibility studies is very high (350:1 for the lube project to 1600:1 for the heavy crude projects). Even assuming relatively low probabilities of timely implementation, the projects meet TDP potential export benefit/cost requirements. Therefore, the study recommends that TDP support all three projects at a level of 50 percent of the estimated cost.

  7. Pyrolysis kinetics applied to prediction of oil generation in the Maracaibo Basin, Venezuela

    SciTech Connect (OSTI)

    Sweeney, J.J.; Talukdar, S.; Burnham, A.K.; Vallejos, C.; DGSI, The Woodlands, TX; Lawrence Livermore National Lab., CA; INTEVEP, Filial de Petroleos de Venezuela, SA, Caracas )

    1989-09-01

    We use chemical kinetic parameters for oil generation derived from modified Rock-Eval and Pyromat instruments, coupled with thermal history models, to predict the timing and extent of oil generation in the Maracaibo Basin of Venezuela. The vitrinite reflectance model developed at Lawrence Livermore National Laboratory is used to calibrate thermal history models with measured vitrinite reflectance profiles. We examine the way differences in the kinetic parameters affect predictions of oil maturation in several parts of the basin with different thermal histories. Maturity indicators, such as H/C atomic ratio and API gravity, are compared to the calculated extent of oil generation. We use the comparison to check the accuracy of the coupled oil generation and thermal history models. 20 refs., 13 figs.

  8. Management of a giant deep field: El Furrial Field, Eastern Venezuela

    SciTech Connect (OSTI)

    Pinto, N.; Mengual, R.; Anz, J.; Rodney, C.

    1996-08-01

    The Furrial Field is located in Eastern Venezuela and produces a 25 to 30{degrees} API asphaltenic crude oil from a 1500 ft thick Oligocene sand interval at a depth of 15,000 ft. The bubble point is about 4500 psi as compared to an original pressure of 11,000 psi. Oil in place is approximately 6800 million STB. Currently production is 350 MB/D from 77 well streams (Naricual Formation), and water is being injected at 400 MB/D to maintain pressure. The combination of a volumetric reservoir and asphaltenic nature of crude oil resulted in a rapid decrease in well productivity and reservoir pressure, creating the necessity to maintain reservoir pressure to maximize recovery. Discussed in this paper are the reservoir management techniques and strategies used by Lagoven to develop and operate these complex reservoirs. Acquisition and analysis of these data adequate to properly implement these management techniques are covered in detail.

  9. Venezuela offshore oil and gas production development: Past, present and future

    SciTech Connect (OSTI)

    Perez La Salvia, H.; Schwartz, E.; Contreras, M.; Rodriguez, J.I.; Febres, G.; Gajardo, E.

    1995-12-01

    This paper presents a short history of offshore oil and gas production in Venezuela starting in Lake Maracaibo in 1923. The main emphasis has been the results of the recent R and D and the exploratory offshore programs in areas like Orinoco Delta located in the Atlantic Ocean, Northeast and Northwest Venezuela in the Caribbean sea. In the R and D offshore program the main objectives were: (1) To establish the local environmental, oceanographical, geotechnical and seismicity conditions for the Venezuelan Continental Platform. (2) To give a technical support to the PDVSA Operating Affiliates during the exploratory programs including: (a) to develop accurate drilling vessel positioning systems; (b) evaluation of sea bottom geotechnical conditions for safely operating the jack-ups and drilling vessels involved in the exploratory wells and (c) to identify those areas which because of their special nature require further investigation to establish preliminary type of platforms required for the areas to be developed or to evaluate other solutions proposed by Foreign Consultant Engineering Companies to the PDVSA Operating Affiliated Companies. The main objective of PDVSA for the coming future will be to develop the North of Paria Gas Field through the initially named Christopher Columbus Project now Sucre Gas, S.A., a consortium conformed by LaGoven, S.A. Shell, Exxon and Mitsubishi. objective of this paper is to give an idea of the history of the Venezuelan Oil and Gas Offshore development giving emphasis to the results of the INTEVEP S.A. Red offshore program and to show some results of the particular characteristics of oceanographical, environmental, geotechnical and seismic conditions in the main areas evaluated during the exploratory program: Orinoco Delta, Gulf of Paria and North of Paria.

  10. Role of p53–fibrinolytic system cross-talk in the regulation of quartz-induced lung injury

    SciTech Connect (OSTI)

    Bhandary, Yashodhar P.; Shetty, Shwetha K.; Marudamuthu, Amarnath S.; Fu, Jian; Pinson, Barbara M.; Levin, Jeffrey; Shetty, Sreerama

    2015-03-01

    Silica is the major component of airborne dust generated by wind, manufacturing and/or demolition. Chronic occupational inhalation of silica dust containing crystalline quartz is by far the predominant form of silicosis in humans. Silicosis is a progressive lung disease that typically arises after a very long latency and is a major occupational concern with no known effective treatment. The mechanism of silicosis is not clearly understood. However, silicosis is associated with increased cell death, expression of redox enzymes and pro-fibrotic cytokines and chemokines. Since alveolar epithelial cell (AEC) death and disruption of alveolar fibrinolysis is often associated with both acute and chronic lung injuries, we explored whether p53-mediated changes in the urokinase-type plasminogen activator (uPA) system contributes to silica-induced lung injury. We further sought to determine whether caveolin-1 scaffolding domain peptide (CSP), which inhibits p53 expression, mitigates lung injury associated with exposure to silica. Lung tissues and AECs isolated from wild-type (WT) mice exposed to silica exhibit increased apoptosis, p53 and PAI-1, and suppression of uPA expression. Treatment of WT mice with CSP inhibits PAI-1, restores uPA expression and prevents AEC apoptosis by suppressing p53, which is otherwise induced in mice exposed to silica. The process involves CSP-mediated inhibition of serine-15 phosphorylation of p53 by inhibition of protein phosphatase 2A-C (PP2A-C) interaction with silica-induced caveolin-1 in AECs. These observations suggest that changes in the p53–uPA fibrinolytic system cross-talk contribute to lung injury caused by inhalation of silica dust containing crystalline quartz and is protected by CSP by targeting this pathway. - Highlights: • Chronic exposure to quartz dusts is a major cause of lung injury and silicosis. • The survival of patients with silicosis is bleak due to lack of effective treatments. • This study defines a new role of

  11. Radiation-Induced Upregulation of Gene Expression From Adenoviral Vectors Mediated by DNA Damage Repair and Regulation

    SciTech Connect (OSTI)

    Nokisalmi, Petri; Rajecki, Maria; Pesonen, Sari; Escutenaire, Sophie; Soliymani, Rabah; Tenhunen, Mikko; Ahtiainen, Laura; Hemminki, Akseli

    2012-05-01

    Purpose: In the present study, we evaluated the combination of replication-deficient adenoviruses and radiotherapy in vitro. The purpose of the present study was to analyze the mechanism of radiation-mediated upregulation of adenoviral transgene expression. Methods and Materials: Adenoviral transgene expression (luciferase or green fluorescent protein) was studied with and without radiation in three cell lines: breast cancer M4A4-LM3, prostate cancer PC-3MM2, and lung cancer LNM35/enhanced green fluorescent protein. The effect of the radiation dose, modification of the viral capsid, and five different transgene promoters were studied. The cellular responses were studied using mass spectrometry and immunofluorescence analysis. Double strand break repair was modulated by inhibitors of heat shock protein 90, topoisomerase-I, and DNA protein kinase, and transgene expression was measured. Results: We found that a wide range of radiation doses increased adenoviral transgene expression regardless of the cell line, transgene, promoter, or viral capsid modification. Treatment with adenovirus, radiation, and double strand break repair inhibitors resulted in persistence of double strand breaks and subsequent increases in adenovirus transgene expression. Conclusions: Radiation-induced enhancement of adenoviral transgene expression is linked to DNA damage recognition and repair. Radiation induces a global cellular response that results in increased production of RNA and proteins, including adenoviral transgene products. This study provides a mechanistic rationale for combining radiation with adenoviral gene delivery.

  12. Hypoxia induced E-cadherin involving regulators of Hippo pathway due to HIF-1α stabilization/nuclear translocation in bone metastasis from breast carcinoma

    SciTech Connect (OSTI)

    Maroni, Paola; Matteucci, Emanuela; Drago, Lorenzo; Banfi, Giuseppe; Bendinelli, Paola; Desiderio, Maria Alfonsina

    2015-01-15

    The present study deals with the molecular mechanisms involved in the regulation of E-cadherin expression under hypoxia, because the adjustment of the amount of E-cadherin due to physical stimuli of the microenvironment might influence the colonization of metastasis to skeleton. We analyzed the effect of 1% oxygen tension, that is similar to that encountered in the bone marrow by metastatic cells spreading from breast carcinoma. The purpose was to evaluate the hypoxia-orchestrated control of E-cadherin transactivation via hypoxia inducible factor-1 (HIF-1) and peroxisome proliferator activated receptor-γ (PPARγ), and the involvement of Hippo pathway members, as regulators of transcription factors. To give a translational significance to the study, we took into consideration human pair-matched ductal breast carcinoma and bone metastasis: E-cadherin and Wwox were expressed in bone metastasis but not in breast carcinoma, while HIF-1α and TAZ seemed localized principally in nuclei of metastasis and were found in all cell compartments of breast carcinoma. A close examination of the regulatory mechanisms underlying E-cadherin expression in bone metastasis was done in 1833 clone derived from MDA-MB231 cells. Hypoxia induced E-cadherin only in 1833 clone, but not in parental cells, through HIF-1 and PPARγ activities, while Wwox decreased. Since Wwox was highly expressed in bone metastasis, the effect of ectopic Wwox was evaluated, and we showed E-cadherin transactivation and enhanced invasiveness in WWOX transfected 1833 cells. Also, hypoxia was additive with ectopic Wwox remarkably enhancing HIF-1α nuclear shuttle and accumulation due to the lengthening of the half-life of HIF-1α protein; under this experimental condition HIF-1α appeared as a slower migrated band compared with control, in agreement with the phosphorylation state. The in vitro data strongly supported the almost exclusive presence of HIF-1α in nuclei of human-bone metastasis. Thus, we identified

  13. The Venezuelan gas industry. Venezuela and other South American countries: Impact on imports into the U.S.

    SciTech Connect (OSTI)

    Mantellini, R.

    1995-11-01

    The role of Venezuela as a supplier of natural gas and derivative products to international markets will experience significant growth in the medium to long term, in the context of expected market opportunities and the development plans envisaged regarding crude oil production. Venezuela has a very large natural gas resource base, which presently amounts to 287 trillion cubic feet (TCF) in terms of proven, probable and possible reserves. Local consumption is highly concentrated in the oil, petrochemicals, aluminum, steel and electricity generation sectors. At the current consumption level of 1.1 TCF/year, proven reserves would supply the country`s requirements for over 120 years. Probable and possible reserves would more than double this figure. Certainly, this is an indication that one is dealing with a gas surplus country with significant potential for growth towards the exports markets. In this regard, Venezuela`s competitive position is further strengthened by the fact that a large portion of its reserves are associated to crude oil, which allows for low production and handling costs, and a relatively high liquid content. It is expected that the natural gas industry will grow rapidly over the coming years. A significant number of gas projects will be developed, including the expansion of existing ones and the construction of new facilities for recovery of natural gas liquids, the expansion of city methane networks replacing LPG as a domestic and industrial fuel, the construction of ethane recovery units for petrochemical uses, etc., all of which represent an additional liquids production of more than 100 {times} 10{sup 3} bbl/d that could be exported to the US and The Caribbean.

  14. Modeling of horizontal well and lifting mechanisms to improve ultimate recovery in a depleted field in Lake Maracaibo, Venezuela

    SciTech Connect (OSTI)

    Saputelli, L.; Mata, T.; Jimenez, Z.

    1995-12-31

    Recovery of the remaining reserve of millions of oil barrels is inhibited by depleted reservoir pressures and existing exploitation policies in Lower Lagunillas Reservoir in Lake Maracaibo, Venezuela. Numerical simulation results indicated that proper production and reservoir management policies such as, controlled drawdown, producing wells at rates below the critical rates, low gas-oil-ratio production will promote efficient gravity segregation process, and subsequent optimum final recovery. Combination of infill horizontal wells and adequate lifting mechanisms yielded the recovery of additional reserves.

  15. Activation of PPAR{delta} up-regulates fatty acid oxidation and energy uncoupling genes of mitochondria and reduces palmitate-induced apoptosis in pancreatic {beta}-cells

    SciTech Connect (OSTI)

    Wan, Jun; Jiang, Li; Lue, Qingguo; Ke, Linqiu; Li, Xiaoyu; Tong, Nanwei

    2010-01-15

    Recent evidence indicates that decreased oxidative capacity, lipotoxicity, and mitochondrial aberrations contribute to the development of insulin resistance and type 2 diabetes. The goal of this study was to investigate the effects of peroxisome proliferator-activated receptor {delta} (PPAR{delta}) activation on lipid oxidation, mitochondrial function, and insulin secretion in pancreatic {beta}-cells. After HIT-T15 cells (a {beta}-cell line) were exposed to high concentrations of palmitate and GW501516 (GW; a selective agonist of PPAR{delta}), we found that administration of GW increased the expression of PPAR{delta} mRNA. GW-induced activation of PPAR{delta} up-regulated carnitine palmitoyltransferase 1 (CPT1), long-chain acyl-CoA dehydrogenase (LCAD), pyruvate dehydrogenase kinase 4 (PDK4), and uncoupling protein 2 (UCP2); alleviated mitochondrial swelling; attenuated apoptosis; and reduced basal insulin secretion induced by increased palmitate in HIT cells. These results suggest that activation of PPAR{delta} plays an important role in protecting pancreatic {beta}-cells against aberrations caused by lipotoxicity in metabolic syndrome and diabetes.

  16. THAP5 is a DNA-binding transcriptional repressor that is regulated in melanoma cells during DNA damage-induced cell death

    SciTech Connect (OSTI)

    Balakrishnan, Meenakshi P.; Cilenti, Lucia; Ambivero, Camilla; Goto, Yamafumi; Takata, Minoru; Turkson, James; Li, Xiaoman Shawn; Zervos, Antonis S.

    2011-01-07

    Research highlights: {yields} THAP5 is a DNA-binding protein and a transcriptional repressor. {yields} THAP5 is induced in melanoma cells upon exposure to UV or treatment with cisplatin. {yields} THAP5 induction correlates with the degree of apoptosis in melanoma cell population. {yields} THAP5 is a pro-apoptotic protein involved in melanoma cell death. -- Abstract: THAP5 was originally isolated as a specific interactor and substrate of the mitochondrial pro-apoptotic Omi/HtrA2 protease. It is a human zinc finger protein characterized by a restricted pattern of expression and the lack of orthologs in mouse and rat. The biological function of THAP5 is unknown but our previous studies suggest it could regulate G2/M transition in kidney cells and could be involved in human cardiomyocyte cell death associated with coronary artery disease (CAD). In this report, we expanded our studies on the properties and function of THAP5 in human melanoma cells. THAP5 was expressed in primary human melanocytes as well as in all melanoma cell lines that were tested. THAP5 protein level was significantly induced by UV irradiation or cisplatin treatment, conditions known to cause DNA damage. The induction of THAP5 correlated with a significant increase in apoptotic cell death. In addition, we show that THAP5 is a nuclear protein that could recognize and bind a specific DNA motif. THAP5 could also repress the transcription of a reporter gene in a heterologous system. Our work suggests that THAP5 is a DNA-binding protein and a transcriptional repressor. Furthermore, THAP5 has a pro-apoptotic function and it was induced in melanoma cells under conditions that promoted cell death.

  17. Rejuvenation of a giant oil field-Quiriquire Field, Venezuela: A team approach

    SciTech Connect (OSTI)

    Friestad, H.; Hull, R.; Miller, D.

    1996-08-01

    Quiriquire field is located in the Maturin basin of eastern Venezuela, at the southeastern corner of the Serrania del Interior mountain range. Since its discovery in 1928, the field has produced over 750 MMBO from a stratigraphically trapped, shallow, Pliocene alluvial fan (Quiriquire Formation). A deep oil zone, the Los Jabillos sand of Oligocene age, was discovered in 1952 on a deep thrust anticline situated below the shallow oil field. Both zones potentially have significant reserves yet to be recovered. In 1994, Maxus, BP, and Otepi began working with Lagoven to rejuvenate oil production from both zones. Maxus, as operator, has been utilizing geologists, geophysicists, petrophysicists, and reservoir, drilling, construction, and environmental engineers, working together as a team, to fully evaluate the most economic methods of developing these remaining reserves. A program of reactivation and recompletion of old wells, as well as the drilling of deviated infill wells, stepouts, and new exploration plays has been formulated by the team. A staged approach to the broad program will enable Maxus to prioritize those projects which have the best economic return. The use of new technology in seismic, drilling, logging, and completions is expected to improve the recovery of oil over previous conventional methods. Using modern technology to enhance the understanding of the structural and stratigraphic trapping mechanisms, the team has defined and built structural and seismic models to help identify possible productive reservoirs.

  18. Influence of Mesozoic age structure on Miocene tectonic development in NE Anzoategui, Eastern Venezuela Basin

    SciTech Connect (OSTI)

    Sadler, P.; White, S.

    1996-08-01

    Structure within and surrounding the Quiamare-La Ceiba region, Eastern Venezuela Basin, is dominated by two major thrust fault systems. They were generated during Early-Middle Miocene time in response to oblique convergence of the Caribbean and South American plates. They are. respectively, the SE vergent NE-SW oriented Anaco fault system, and the SSE vergent ENE-WSW oriented Pirital fault system. The major structural feature associated with each fault system is a basement cored ramp anticline. New seismic data provides evidence that contributes to a better understanding of the sequence of tectonic development within and surrounding the Quiamare-La Ceiba region. Compressional structures in both the hanging wall and the footwall of the Pirital fault system appear to be inverted normal faults, that were previously active during Mesozoic time along the northern South America passive margin. A conjugate set of strike-slip faults is also present. They are oriented NNW-SSE, parallel to the Urica lineation, and SSW-NNE, respectively. A Mesozoic origin for these faults is suggested. Post-compressional relaxation during Plio-Pleistocene time resulted in the development of shallow, small scale normal faults. These normal faults appear to be localized by structural adjustments along the strike-slip fault sets. Existing oil and gas production within the Quiamare-La Ceiba region is from localized structural closures. Strike-slip faults dissect the prevailing structural grain, and may provide an additional hydrocarbon trapping mechanism.

  19. Geological and geophysical evaluation of the Naricual Formation, Musipan-El Carito area, eastern Venezuela basin

    SciTech Connect (OSTI)

    Abud, J.; Oviedo, P.; Hernandex, J.; Garcia, E.; Escalona, N. )

    1990-05-01

    The recent giant oil discoveries in the lower Tertiary sedimentary sequence of the Monagas overthrust belt, Eastern Venezuela basin require short- and medium-term development plans based on technical production geological studies and models. The present study consists of a detailed geological and geophysical evaluation to define the reservoir in the producing Naricual Formation in the Musipan-El Carito areas, located west of El Furrial oil field. Due to its geological and reservoir characteristics, the northern Monagas area is considered as the top priority production alternative for the Venezuelan oil industry for the next 20 yr. The structural pattern of the area is related to major compressional stress applied northwest and from the northeast. Two fault systems are associated with this compression: (1) first-order, east-west-trending reverse faults dipping to the north, and (2) second-order, north-south-trending right-lateral strike-slip faults. The proposed sedimentological model is that of a paleodelta prograding from north to south. The depositional environment ranges from internal to continental shelf (barrier bars, tidal and distributary channels, lagoonal and associated facies). The STOIP in the Naricual Formation is 4.8 billion bbls, 52% of which is light-grade oil and 48% is medium-grade oil. The results of reservoir pressure analyses suggest lateral communication within the Naricual Formation between the Furrial and Musipan areas and give evidence of a permeability barrier between the Furrial-Musipan and El Carito areas. Depth vs. API gravity plots indicate a direct relationship between crude oil type and depth. The application of the results of the integrated reservoir studies gives precise answers to the production behavior of wells. An adjusted development plan is now under way to guarantee a rational, optimum recovery of the oil reserves.

  20. Developing a marginal field using new techniques-South Monagas Unit, Venezuela

    SciTech Connect (OSTI)

    Skirvin, T.M.; Sven Hagen, E.; McGee, R.A.; Hinrichs, P.D.; Medina, P.A.

    1996-12-31

    In 1992 the Venezuelan national oil company, PDVSA, awarded operating service agreements to foreign oil companies for reactivation of marginal oil fields. The South Monagas Unit contains three oil and gas fields, Uracoa, Bombal, and Tucupita, that were not producing prior to the award of the contract As of October 1995, production from Uraroa had exceeded 20 MBbls/day of heavy oil from 26 vertical and 11 horizontal wells. Initial uncertainties about heavy oil treatment capability, water and gas production, oil flow rates, and ultimate recoverable reserves led to a phased development plan that has incrementally reduced the risk of financial exposure over time. The first phase of development utilized conventional geologic techniques and vertical wells to test treatment facilities, mud and gravel-pack technologies, and flow rates. Positive results led to the next phase of development which focused on reservoir performance and well optimization. A horizontal well drilling program was implemented in December 1993. A milestone in this program was the first gravel-pack horizontal well in Venezuela, completed in February, 1995. A pilot 2-D seismic program in late 1994 confined that high-quality seismic could be acquired to significantly enhance the development of Uracoa. A 175 W 3-D survey was shot and processed in mid-1995. Concurrently, borehole imaging logs were acquired in vertical wells to determine internal reservoir heterogeneity and sand depositional models. The sequence stratigraphic model that evolved, based on outcrop field analogs, 3-D seismic stratigraphy, and regional well control, is being used to optimize field development. In addition, new exploration concepts are being tested without risk using strategically located water injection wells as test wells.

  1. Sedimentary and tectonic controls on oil occurrences in the traditional producing area, Barinas Subbasin, Western Venezuela

    SciTech Connect (OSTI)

    Daal, J.; Martinez, G.; Salas, J. )

    1996-01-01

    A Stratigraphic and Tectonic model explains the oil-field locations in the Traditional Producing Area of the Barinas Subbasin, Western Venezuela. The database for the model includes a 585-km[sup 2] 3-D seismic survey, as well as petrophysical, lithologic and biostratigraphic data from Cretaceous and Tertiary sediments. A long-term relative sea level rise from Albian through Campanian (Cretaceous) time, coincident with passive-margin basin subsidence, resulted in onlap of marginal marine sands and marine-shelf limestones and shales over crystalline metamorphic rocks of the Guayana Shield Basement. Facies changes in the Cretaceous Aguardiente, Escandalosa, and Navay Formations are related mainly to eustatic sea level changes. A tectonic pulse deformed these sediments in Late Maastrichtian to Paleocene time. An erosional unconformity that developed atop this deformed Cretaceous section relates to tectonic uplift and not to sea-level change. Onlap of Middle Eocene marine transgressive Gobernador Fm. sands and Masparrito Fm. limestones over this unconformity was driven by increased tectonic subsidence. Accelerated tectonic subsidence drowned the Masparrito carbonate platform and led to deposition of a condensed section within the lower Paguey Formation; this condensed section marks a tectonic Maximum Flooding Surface not related to eustatic sea level change. After deposition of the Eocene Paguey, and just prior to deposition of the Oligo-Miocene Parangula Formation, a second tectonic event reactivated older faults and led to growth of structural traps for Cretaceous and Eocene reservoirs. Both tectonic and eustatic events have combined to control oil occurrence in the Barinas Subbasin.

  2. Sedimentary and tectonic controls on oil occurrences in the traditional producing area, Barinas Subbasin, Western Venezuela

    SciTech Connect (OSTI)

    Daal, J.; Martinez, G.; Salas, J.

    1996-12-31

    A Stratigraphic and Tectonic model explains the oil-field locations in the Traditional Producing Area of the Barinas Subbasin, Western Venezuela. The database for the model includes a 585-km{sup 2} 3-D seismic survey, as well as petrophysical, lithologic and biostratigraphic data from Cretaceous and Tertiary sediments. A long-term relative sea level rise from Albian through Campanian (Cretaceous) time, coincident with passive-margin basin subsidence, resulted in onlap of marginal marine sands and marine-shelf limestones and shales over crystalline metamorphic rocks of the Guayana Shield Basement. Facies changes in the Cretaceous Aguardiente, Escandalosa, and Navay Formations are related mainly to eustatic sea level changes. A tectonic pulse deformed these sediments in Late Maastrichtian to Paleocene time. An erosional unconformity that developed atop this deformed Cretaceous section relates to tectonic uplift and not to sea-level change. Onlap of Middle Eocene marine transgressive Gobernador Fm. sands and Masparrito Fm. limestones over this unconformity was driven by increased tectonic subsidence. Accelerated tectonic subsidence drowned the Masparrito carbonate platform and led to deposition of a condensed section within the lower Paguey Formation; this condensed section marks a tectonic Maximum Flooding Surface not related to eustatic sea level change. After deposition of the Eocene Paguey, and just prior to deposition of the Oligo-Miocene Parangula Formation, a second tectonic event reactivated older faults and led to growth of structural traps for Cretaceous and Eocene reservoirs. Both tectonic and eustatic events have combined to control oil occurrence in the Barinas Subbasin.

  3. Developing a marginal field using new techniques-South Monagas Unit, Venezuela

    SciTech Connect (OSTI)

    Skirvin, T.M.; Sven Hagen, E.; McGee, R.A.; Hinrichs, P.D. ); Medina, P.A. )

    1996-01-01

    In 1992 the Venezuelan national oil company, PDVSA, awarded operating service agreements to foreign oil companies for reactivation of marginal oil fields. The South Monagas Unit contains three oil and gas fields, Uracoa, Bombal, and Tucupita, that were not producing prior to the award of the contract As of October 1995, production from Uraroa had exceeded 20 MBbls/day of heavy oil from 26 vertical and 11 horizontal wells. Initial uncertainties about heavy oil treatment capability, water and gas production, oil flow rates, and ultimate recoverable reserves led to a phased development plan that has incrementally reduced the risk of financial exposure over time. The first phase of development utilized conventional geologic techniques and vertical wells to test treatment facilities, mud and gravel-pack technologies, and flow rates. Positive results led to the next phase of development which focused on reservoir performance and well optimization. A horizontal well drilling program was implemented in December 1993. A milestone in this program was the first gravel-pack horizontal well in Venezuela, completed in February, 1995. A pilot 2-D seismic program in late 1994 confined that high-quality seismic could be acquired to significantly enhance the development of Uracoa. A 175 W 3-D survey was shot and processed in mid-1995. Concurrently, borehole imaging logs were acquired in vertical wells to determine internal reservoir heterogeneity and sand depositional models. The sequence stratigraphic model that evolved, based on outcrop field analogs, 3-D seismic stratigraphy, and regional well control, is being used to optimize field development. In addition, new exploration concepts are being tested without risk using strategically located water injection wells as test wells.

  4. Inhibition of SH2-domain-containing inositol 5-phosphatase (SHIP2) ameliorates palmitate induced-apoptosis through regulating Akt/FOXO1 pathway and ROS production in HepG2 cells

    SciTech Connect (OSTI)

    Gorgani-Firuzjaee, Sattar; Adeli, Khosrow; Meshkani, Reza

    2015-08-21

    The serine–threonine kinase Akt regulates proliferation and survival by phosphorylating a network of protein substrates; however, the role of a negative regulator of the Akt pathway, the SH2-domain-containing inositol 5-phosphatase (SHIP2) in apoptosis of the hepatocytes, remains unknown. In the present study, we studied the molecular mechanisms linking SHIP2 expression to apoptosis using overexpression or suppression of SHIP2 gene in HepG2 cells exposed to palmitate (0.5 mM). Overexpression of the dominant negative mutant SHIP2 (SHIP2-DN) significantly reduced palmitate-induced apoptosis in HepG2 cells, as these cells had increased cell viability, decreased apoptotic cell death and reduced the activity of caspase-3, cytochrome c and poly (ADP-ribose) polymerase. Overexpression of the wild-type SHIP2 gene led to a massive apoptosis in HepG2 cells. The protection from palmitate-induced apoptosis by SHIP2 inhibition was accompanied by a decrease in the generation of reactive oxygen species (ROS). In addition, SHIP2 inhibition was accompanied by an increased Akt and FOXO-1 phosphorylation, whereas overexpression of the wild-type SHIP2 gene had the opposite effects. Taken together, these findings suggest that SHIP2 expression level is an important determinant of hepatic lipoapotosis and its inhibition can potentially be a target in treatment of hepatic lipoapoptosis in diabetic patients. - Highlights: • Lipoapoptosis is the major contributor to the development of NAFLD. • The PI3-K/Akt pathway regulates apoptosis in different cells. • The role of negative regulator of this pathway, SHIP2 in lipoapoptosis is unknown. • SHIP2 inhibition significantly reduces palmitate-induced apoptosis in HepG2 cells. • SHIP2 inhibition prevents palmitate induced-apoptosis by regulating Akt/FOXO1 pathway.

  5. Quinacrine induces apoptosis in human leukemia K562 cells via p38 MAPK-elicited BCL2 down-regulation and suppression of ERK/c-Jun-mediated BCL2L1 expression

    SciTech Connect (OSTI)

    Changchien, Jung-Jung; Chen, Ying-Jung; Huang, Chia-Hui; Cheng, Tian-Lu; Lin, Shinne-Ren; Chang, Long-Sen

    2015-04-01

    Although previous studies have revealed the anti-cancer activity of quinacrine, its effect on leukemia is not clearly resolved. We sought to explore the cytotoxic effect and mechanism of quinacrine action in human leukemia K562 cells. Quinacrine induced K562 cell apoptosis accompanied with ROS generation, mitochondrial depolarization, and down-regulation of BCL2L1 and BCL2. Upon exposure to quinacrine, ROS-mediated p38 MAPK activation and ERK inactivation were observed in K562 cells. Quinacrine-induced cell death and mitochondrial depolarization were suppressed by the p38MAPK inhibitor SB202190 and constitutively active MEK1 over-expression. Activation of p38 MAPK was shown to promote BCL2 degradation. Further, ERK inactivation suppressed c-Jun-mediated transcriptional expression of BCL2L1. Over-expression of BCL2L1 and BCL2 attenuated quinacrine-evoked mitochondrial depolarization and rescued the viability of quinacrine-treated cells. Taken together, our data indicate that quinacrine-induced K562 cell apoptosis is mediated through mitochondrial alterations triggered by p38 MAPK-mediated BCL2 down-regulation and suppression of ERK/c-Jun-mediated BCL2L1 expression. - Highlights: • Quinacrine induces K562 cell apoptosis via down-regulation of BCL2 and BCL2L1. • Quinacrine induces p38 MAPK activation and ERK inactivation in K562 cells. • Quinacrine elicits p38 MAPK-mediated BCL2 down-regulation. • Quinacrine suppresses ERK/c-Jun-mediated BCL2L1 expression.

  6. Venezuela-MEM/USA-DOE Fossil Energy Report IV-11: Supporting technology for enhanced oil recovery - EOR thermal processes

    SciTech Connect (OSTI)

    Venezuela

    2000-04-06

    This report contains the results of efforts under the six tasks of the Tenth Amendment anti Extension of Annex IV, Enhanced Oil Recovery Thermal Processes of the Venezuela/USA Energy Agreement. This report is presented in sections (for each of the six Tasks) and each section contains one or more reports that were prepared to describe the results of the effort under each of the Tasks. A statement of each Task, taken from the Agreement Between Project Managers, is presented on the first page of each section. The Tasks are numbered 68 through 73. The first through tenth report on research performed under Annex IV Venezuela MEM/USA-DOE Fossil Energy Report Number IV-1, IV-2, IV-3, IV-4, IV-5, IV-6, IV-7, IV-8, IV-9, IV-10 contain the results of the first 67 Tasks. These reports are dated April 1983, August 1984, March 1986, July 1987, November 1988, December 1989, October 1991, February 1993, March 1995, and December 1997, respectively.

  7. Oil- and gas-in-place assessment under geological uncertainty, Jobal-Zuron area, Guarico subbasin, central Venezuela

    SciTech Connect (OSTI)

    Martinez, G.

    1989-03-01

    Oil- and gas-in-place assessment has been a major problem in the evaluation of the Jobal-Zuron area of the Guarico subbasin of the Eastern Venezuela basin. The area is now undergoing an integrated appraisal in order to determine its economic viability for supplying the Venezuelan domestic gas market. The area has been known as a gas producer from the Miocene Chaguaramas Formation. Currently stated reserves are estimated at 21.7 million bbl of condensate and 313.8 billion ft/sup 3/ of gas; daily production is 23 million ft/sup 3/ of gas from nine wells. Recent tests and appaisals would increase reserves to 60 million bbl of condensate and 888.6 billion ft/sup 3/ of gas and daily production up to 4.2 million ft/sup 3/ of gas per well. Although the Venezuelan domestic gas market is increasing, the Jobal-Zuron area is still lacking deep geological and reserve definition. The area is, geologically speaking, one of the most complex in Venezuela from both the stratigraphic and structural points of view. The Guarico subbasin was tectonically very active during the upper Miocene-Pliocene; compaction and diagenesis are the main factors affecting porosity.

  8. Cyanidin-3-glucoside inhibits UVB-induced oxidative damage and inflammation by regulating MAP kinase and NF-κB signaling pathways in SKH-1 hairless mice skin

    SciTech Connect (OSTI)

    Pratheeshkumar, Poyil; Son, Young-Ok; Wang, Xin; Divya, Sasidharan Padmaja; Joseph, Binoy; Hitron, John Andrew; Wang, Lei; Kim, Donghern; Yin, Yuanqin; Roy, Ram Vinod; Lu, Jian; Zhang, Zhuo; Wang, Yitao; and others

    2014-10-01

    Skin cancer is one of the most commonly diagnosed cancers in the United States. Exposure to ultraviolet-B (UVB) radiation induces inflammation and photocarcinogenesis in mammalian skin. Cyanidin-3-glucoside (C3G), a member of the anthocyanin family, is present in various vegetables and fruits especially in edible berries, and displays potent antioxidant and anticarcinogenic properties. In this study, we have assessed the in vivo effects of C3G on UVB irradiation induced chronic inflammatory responses in SKH-1 hairless mice, a well-established model for UVB-induced skin carcinogenesis. Here, we show that C3G inhibited UVB-induced skin damage and inflammation in SKH-1 hairless mice. Our results indicate that C3G inhibited glutathione depletion, lipid peroxidation and myeloperoxidation in mouse skin by chronic UVB exposure. C3G significantly decreased the production of UVB-induced pro-inflammatory cytokines, such as IL-6 and TNF-α, associated with cutaneous inflammation. Likewise, UVB-induced inflammatory responses were diminished by C3G as observed by a remarkable reduction in the levels of phosphorylated MAP kinases, Erk1/2, p38, JNK1/2 and MKK4. Furthermore, C3G also decreased UVB-induced cyclooxygenase-2 (COX-2), PGE{sub 2} and iNOS levels, which are well-known key mediators of inflammation and cancer. Treatment with C3G inhibited UVB-induced nuclear translocation of NF-κB and degradation of IκBα in mice skin. Immunofluorescence assay revealed that topical application of C3G inhibited the expression of 8-hydroxy-2′-deoxyguanosine, proliferating cell nuclear antigen, and cyclin D1 in chronic UVB exposed mouse skin. Collectively, these data indicates that C3G can provide substantial protection against the adverse effects of UVB radiation by modulating UVB-induced MAP kinase and NF-κB signaling pathways. - Highlights: • C3G inhibited UVB-induced oxidative damage and inflammation. • C3G inhibited UVB-induced COX-2, iNOS and PGE{sub 2} production. • C3G

  9. Acquisition Regulation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Career Development, Contracting Authority and Responsibilities Acquisition Regulation ... file, except for any documents required by regulation to be maintained in paper copy. ...

  10. Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Information Center Regulations Regulations Personnel Security Regulations Click Here FOIA Regulations Click Here Privacy Act Regulations Click Here Whistleblower Protection ...

  11. VOLTAGE REGULATOR

    DOE Patents [OSTI]

    Von Eschen, R.L.; Scheele, P.F.

    1962-04-24

    A transistorized voltage regulator which provides very close voitage regulation up to about 180 deg F is described. A diode in the positive line provides a constant voltage drop from the input to a regulating transistor emitter. An amplifier is coupled to the positive line through a resistor and is connected between a difference circuit and the regulating transistor base which is negative due to the difference in voltage drop across thc diode and the resistor so that a change in the regulator output causes the amplifier to increase or decrease the base voltage and current and incrcase or decrease the transistor impedance to return the regulator output to normal. (AEC)

  12. {gamma}-aminobutyric acid{sub A} (GABA{sub A}) receptor regulates ERK1/2 phosphorylation in rat hippocampus in high doses of Methyl Tert-Butyl Ether (MTBE)-induced impairment of spatial memory

    SciTech Connect (OSTI)

    Zheng Gang; Zhang Wenbin; Zhang Yun; Chen Yaoming; Liu Mingchao; Yao Ting; Yang Yanxia; Zhao Fang; Li Jingxia; Huang Chuanshu; Luo Wenjing Chen Jingyuan

    2009-04-15

    Experimental and occupational exposure to Methyl Tert-Butyl Ether (MTBE) has been reported to induce neurotoxicological and neurobehavioral effects, such as headache, nausea, dizziness, and disorientation, etc. However, the molecular mechanisms involved in MTBE-induced neurotoxicity are still not well understood. In the present study, we investigated the effects of MTBE on spatial memory and the expression and function of GABA{sub A} receptor in the hippocampus. Our results demonstrated that intraventricular injection of MTBE impaired the performance of the rats in a Morris water maze task, and significantly increased the expression of GABA{sub A} receptor {alpha}1 subunit in the hippocampus. The phosphorylation of ERK1/2 decreased after the MTBE injection. Furthermore, the decreased ability of learning and the reduction of phosphorylated ERK1/2 level of the MTBE-treated rats was partly reversed by bicuculline injected 30 min before the training. These results suggested that MTBE exposure could result in impaired spatial memory. GABA{sub A} receptor may play an important role in the MTBE-induced impairment of learning and memory by regulating the phosphorylation of ERK in the hippocampus.

  13. Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function During Nanoparticle-Induced Oxidative Stress

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Duan, Jicheng; Kodali, Vamsi K.; Gaffrey, Matthew J.; Guo, Jia; Chu, Rosalie K.; Camp, David G.; Smith, Richard D.; Thrall, Brian D.; Qian, Wei-Jun

    2015-12-23

    Engineered nanoparticles (ENPs) are emerging functional materials increasingly utilized for commercial and medical applications. Due to the potential hazard effects of ENPs to human health, it is significant to assess and understand the underlying mechanisms of nanotoxicity. Here, we investigate protein S-glutathionylation (SSG) as an underlying regulatory mechanism for ENP-induced oxidative stress in macrophages by applying a recently developed quantitative redox proteomics approach for site-specific measurements of SSG. Three high-volume production ENPs (SiO2, Fe3O4 and CoO) were selected as representative ENPs with low, moderate, and high reactive oxygen species (ROS) activity, respectively. Among these nanoparticles, we observe that CoO ledmore » to the most significant dose-dependent oxidative stress and increase of protein SSG modifications in macrophages. Our site-specific SSG changes highlighted a broad set of redox sensitive proteins and their specific Cys residues potentially implicated in stress response. Functional analysis revealed that the most significantly enriched functional categories for SSG-modified proteins were stress response, cellular structure change, and cell death or survival. Moreover, ENPs-induce oxidative stress levels (CoO > Fe3O4 > SiO2) were found to correlate well with the levels of impairment of macrophage phagocytic activity and the overall degrees of increases in SSG. RNA silencing knockdown experiment of glutaredoxin 1 (Grx1) also led to a decreased phagocytic activity in macrophages, which suggested a regulatory role of SSG in phagocytosis. Together, the results provided valuable insights of protein SSG as a potential regulatory mechanism in response to nanomaterial-induced oxidative stress and immunity dysfunction.« less

  14. Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function During Nanoparticle-Induced Oxidative Stress

    SciTech Connect (OSTI)

    Duan, Jicheng; Kodali, Vamsi K.; Gaffrey, Matthew J.; Guo, Jia; Chu, Rosalie K.; Camp, David G.; Smith, Richard D.; Thrall, Brian D.; Qian, Weijun

    2015-12-23

    Engineered nanoparticles (ENPs) are emerging functional materials increasingly utilized for commercial and medical applications. Due to the potential hazard effects of ENPs to human health, it is significant to assess and understand the underlying mechanisms of nanotoxicity. Herein, we investigate protein S-glutathionylation (SSG) as an underlying regulatory mechanism for ENP-induced oxidative stress in macrophages by applying a recently developed quantitative redox proteomics approach for site-specific measurements of SSG. Three high-volume production ENPs (SiO2, Fe3O4 and CoO) were selected as representative ENPs with low, moderate, and high reactive oxygen species (ROS) activity, respectively. Among these nanoparticles, we observe that CoO led to the most significant dose-dependent oxidative stress and increase of protein SSG modifications in macrophages. Our site-specific SSG changes highlighted a broad set of redox sensitive proteins and their specific Cys residues potentially implicated in stress response. Functional analysis revealed that the most significantly enriched functional categories for SSG-modified proteins were stress response, cellular structure change, and cell death or survival. Moreover, ENPs-induce oxidative stress levels (CoO > Fe3O4 > SiO2) were found to correlate well with the levels of impairment of macrophage phagocytic activity and the overall degrees of increases in SSG. RNA silencing knockdown experiment of glutaredoxin 1 (Grx1) also led to a decreased phagocytic activity in macrophages, which suggested a regulatory role of SSG in phagocytosis. Together, the results provided valuable insights of protein SSG as a potential regulatory mechanism in response to nanomaterial-induced oxidative stress and immunity dysfunction.

  15. Evaluating oil quality and monitoring production from heavy oil reservoirs using geochemical methods: Application to the Boscan Field, Venezuela

    SciTech Connect (OSTI)

    Kaufman, R.L.; Noguera, V.H.; Bantz, D.M.; Rodriguez, R.

    1996-08-01

    Many oil fields worldwide contain heavy oil in one or more reservoir units. The low gravity of these oils is most frequently due to biodegradation and/or low maturity. The challenge is to find ways to economically recover this oil. Methods which reduce the operating costs of producing heavy oil add significant value to such projects. Geochemical techniques which use the composition of the reservoir fluids as natural tracers offer cost effective methods to assist with reservoir management. The low viscosity and gravity of heavy oil, combined with frequent high water cuts, low flow rates, and the presence of downhole artificial lift equipment, make many conventional production logging methods difficult to apply. Therefore, monitoring production, especially if the produced oil is commingled from multiple reservoirs, can be difficult. Geochemical methods can be used to identify oil/water contacts, tubing string leaks and to allocate production to individual zones from commingled production. An example of a giant heavy oil field where geochemical methods may be applicable is the Boscan Field in Venezuela. Low maturity oil, averaging 10{degrees} API gravity, is produced from the Eocene Upper and Lower Boscan (Miosa) Sands. Geochemical, stratigraphic and engineering data have helped to better define the controls on oil quality within the field, identified new reservoir compartments and defined unique characteristics of the Upper and Lower Boscan oils. This information can be used to identify existing wells in need of workovers due to mechanical problems and to monitor production from new infill wells.

  16. Venezuela-MEM/USA-DOE Fossil Energy Report XIII-1, Supporting Technology for Enhanced Oil Recovery, Microbial EOR

    SciTech Connect (OSTI)

    Ziritt, Jose Luis

    1999-11-03

    The results from Annex XIII of the Cooperative Agreement between the United States Department of Energy (DOE) and the Ministry of Energy and Mines of the Republic of Venezuela (MEMV) have been documented and published with many researchers involved. Integrate comprehensive research programs in the area of Microbial Enhanced Oil Recovery (MEOR) ranged from feasibility laboratory studies to full-scale multi-well field pilots. The objective, to cooperate in a technical exchange of ideas and information was fully met throughout the life of the Annex. Information has been exchanged between the two countries through published reports and technical meetings between experts in both country's research communities. The meetings occurred every two years in locations coincident with the International MEOR conferences & workshops sponsored by DOE (June 1990, University of Oklahoma, September 1992, Brookhaven, September 1995, National Institute of Petroleum and Energy Research). Reports and publications produced during these years are listed in Appendix B. Several Annex managers have guided the exchange through the years. They included Luis Vierma, Jose Luis Zirritt, representing MEMV and E. B. Nuckols, Edith Allison, and Rhonda Lindsey, representing the U.S. DOE. Funding for this area of research remained steady for a few years but decreased in recent years. Because both countries have reduced research programs in this area, future exchanges on this topic will occur through ANNEX XV. Informal networks established between researchers through the years should continue to function between individuals in the two countries.

  17. Approaches to identifying reservoir heterogeneity and reserve growth opportunities from subsurface data: The Oficina Formation, Budare field, Venezuela

    SciTech Connect (OSTI)

    Hamilton, D.S.; Raeuchle, S.K.; Holtz, M.H.

    1997-08-01

    We applied an integrated geologic, geophysical, and engineering approach devised to identify heterogeneities in the subsurface that might lead to reserve growth opportunities in our analysis of the Oficina Formation at Budare field, Venezuela. The approach involves 4 key steps: (1) Determine geologic reservoir architecture; (2) Investigate trends in reservoir fluid flow; (3) Integrate fluid flow trends with reservoir architecture; and (4) Estimate original oil-in-place, residual oil saturation, and remaining mobile oil, to identify opportunities for reserve growth. There are three main oil-producing reservoirs in the Oficina Formation that were deposited in a bed-load fluvial system, an incised valley-fill, and a barrier-strandplain system. Reservoir continuity is complex because, in addition to lateral facies variability, the major Oficina depositional systems were internally subdivided by high-frequency stratigraphic surfaces. These surfaces define times of intermittent lacustrine and marine flooding events that punctuated the fluvial and marginal marine sedimentation, respectively. Syn and post depositional faulting further disrupted reservoir continuity. Trends in fluid flow established from initial fluid levels, response to recompletion workovers, and pressure depletion data demonstrated barriers to lateral and vertical fluid flow caused by a combination of reservoir facies pinchout, flooding shale markers, and the faults. Considerable reserve growth potential exists at Budare field because the reservoir units are highly compartment by the depositional heterogeneity and structural complexity. Numerous reserve growth opportunities were identified in attics updip of existing production, in untapped or incompletely drained compartments, and in field extensions.

  18. Reciprocal Regulation of Hypoxia-Inducible Factor 2α and GLI1 Expression Associated With the Radioresistance of Renal Cell Carcinoma

    SciTech Connect (OSTI)

    Zhou, Jiancheng; Wu, Kaijie; Gao, Dexuan; Zhu, Guodong; Wu, Dapeng; Wang, Xinyang; Chen, Yule; Du, Yuefeng; Song, Wenbin; Ma, Zhenkun; Authement, Craig; Saha, Debabrata; Hsieh, Jer-Tsong; He, Dalin

    2014-11-15

    Purpose: Renal cell carcinoma (RCC) is often considered a radioresistant tumor, but the molecular mechanism underlying its radioresistance is poorly understood. This study explored the roles of hypoxia-inducible factor 2α (HIF2α) and sonic hedgehog (SHH)-GLI1 signaling in mediating the radioresistance of RCC cells and to unveil the interaction between these 2 signaling pathways. Methods and Materials: The activities of SHH-GLI1 signaling pathway under normoxia and hypoxia in RCC cells were examined by real-time polymerase chain reaction, Western blot, and luciferase reporter assay. The expression of HIF2α and GLI1 in RCC patients was examined by immunohistochemistry, and their correlation was analyzed. Furthermore, RCC cells were treated with HIF2α-specific shRNA (sh-HIF2α), GLI1 inhibitor GANT61, or a combination to determine the effect of ionizing radiation (IR) on RCC cells based on clonogenic assay and double-strand break repair assay. Results: RCC cells exhibited elevated SHH-GLI1 activities under hypoxia, which was mediated by HIF2α. Hypoxia induced GLI1 activation through SMO-independent pathways that could be ablated by PI3K inhibitor or MEK inhibitor. Remarkably, the SHH-GLI1 pathway also upregulated HIF2α expression in normoxia. Apparently, there was a positive correlation between HIF2α and GLI1 expression in RCC patients. The combination of sh-HIF2α and GLI1 inhibitor significantly sensitized RCC cells to IR. Conclusions: Cross-talk between the HIF2α and SHH-GLI1 pathways was demonstrated in RCC. Cotargeting these 2 pathways, significantly sensitizing RCC cells to IR, provides a novel strategy for RCC treatment.

  19. Uptake of donor lymphocytes treated with 8-methoxypsoralen and ultraviolet A light by recipient dendritic cells induces CD4{sup +}CD25{sup +}Foxp3{sup +} regulatory T cells and down-regulates cardiac allograft rejection

    SciTech Connect (OSTI)

    Zheng, De-Hua; Dou, Li-Ping; Wei, Yu-Xiang; Du, Guo-Sheng; Zou, Yi-Ping; Song, Ji-Yong; Zhu, Zhi-Dong; Cai, Ming; Qian, Ye-Yong; Shi, Bing-Yi

    2010-05-14

    Extracorporeal photopheresis (ECP) is an effective immunomodulatory therapy and has been demonstrated to be beneficial for graft-vs-host disease and solid-organ allograft rejection. ECP involves reinfusion of a patient's autologous peripheral blood leukocytes treated ex vivo with 8-methoxypsoralen and UVA light radiation (PUVA). Previous studies focused only on ECP treatment of recipient immune cells. Our study is the first to extend the target of ECP treatment to donor immune cells. The results of in vitro co-culture experiments demonstrate uptake of donor PUVA-treated splenic lymphocytes (PUVA-SPs) by recipient immature dendritic cells (DCs). Phagocytosis of donor PUVA-SPs does not stimulate phenotype maturation of recipient DCs. In the same co-culture system, donor PUVA-SPs enhanced production of interleukin-10 and interferon-{gamma} by recipient DCs and impaired the subsequent capability of recipient DCs to stimulate recipient naive T cells. Phagocytosis of donor PUVA-SP (PUVA-SP DCs) by recipient DCs shifted T-cell responses in favor of T helper 2 cells. Infusion of PUVA-SP DCs inhibited cardiac allograft rejection in an antigen-specific manner and induced CD4{sup +}CD25{sup high}Foxp3{sup +} regulatory T cells. In conclusion, PUVA-SP DCs simultaneously deliver the donor antigen and the regulatory signal to the transplant recipient, and thus can be used to develop a novel DC vaccine for negative immune regulation and immune tolerance induction.

  20. Simultaneous determination of thorium, niobium, lead, and zinc by photon-induced x-ray fluorescence of lateritic material

    SciTech Connect (OSTI)

    LaBrecque, J.J.; Adames, D.; Parker, W.C.

    1981-01-01

    A rapid method is presented for the simultaneous determinations of thorium, niobium, lead, and zinc in lateritic material from Cerro Impacto, Estado Bolivar, Venezuela. This technique uses a PDP - 11/05 processor - based photon induced x-ray fluorescence system. The total variations of approximately 5% for concentrations of approximately 1 and 10% for concentrations of approximately 0.1% were obtained with only 500 s of fluorescent time. The values obtained by this method were in agreement with values measured by conventional flame atomic absorption spectroscopy for lead and zinc. The values for thorium measured were in agreement with the reported values for the reference materials supplied by NBL.

  1. Acquisition Regulation

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Rev. 1 Date 09/09/2015 Department of Energy No. FAL 2015-04 Rev. 1 Date 09/09/2015 Financial Assistance Regulations ACQUISITION/FINANCIAL ASSISTANCE LETTER This Acquisition/Financial Assistance Letter is issued under the authority of the Senior Procurement Executive of DOE. It is intended for use by procurement professionals of DOE, primarily Contracting Officers, and other officials of DOE that are involved in the acquisition process. Other parties are welcome to its information, but

  2. Venezuela to double Supermetanol

    SciTech Connect (OSTI)

    1997-04-23

    Pequiven, the petrochemical arm of Venezuelan state oil company PDVSA, is conducting feasibility studies to double the size of its 750,000-m.t./year Supermetanol methanol joint venture with Ecofuel at Jose. The twin unit would be onstream by the end of the decade and would increase Pequiven`s capacity to 2.3 million m.t./year.

  3. Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations Regulations Security Reviews Click Here FOIA Appeals Click Here Whistleblower Protection Click Here Procedural Regulations Click Here Browse Title 10 Energy Click Here

  4. Charge regulation circuit

    DOE Patents [OSTI]

    Ball, Don G.

    1992-01-01

    A charge regulation circuit provides regulation of an unregulated voltage supply in the range of 0.01%. The charge regulation circuit is utilized in a preferred embodiment in providing regulated voltage for controlling the operation of a laser.

  5. Regulators, Requirements, Statutes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regulators, Requirements, Statutes Regulators, Requirements, Statutes The Laboratory must comply with environmental laws and regulations that apply to Laboratory operations. Contact Environmental Communication & Public Involvement P.O. Box 1663 MS M996 Los Alamos, NM 87545 (505) 667-0216 Email Environmental laws and regulations LANL complies with more than 30 state and federal regulations and policies designed to protect human health and the environment. Regulators Regulators Environmental

  6. CO{sub 2} emissions from developing countries: Better understanding the role of Energy in the long term. Volume 2, Argentina, Brazil, Mexico, and Venezuela

    SciTech Connect (OSTI)

    Ketoff, A.; Sathaye, J.; Goldman, N.

    1991-07-01

    Recent years have witnessed a growing recognition of the link between emissions of carbon dioxide (CO{sub 2}) and changes in the global climate. Of all anthropogenic activities, energy production and use generate the single largest portion of these greenhouse gases. Although developing countries currently account for a small share of global carbon emissions, their contribution is increasing rapidly. Due to the rapid expansion of energy demand in these nations, the developing world`s share in global modern energy use rose from 16 to 27 percent between 1970 and 1990. If the growth rates observed over the past 20 years persist energy demand in developing will surpass that in the countries of the Organization for Economic Cooperation and Development (OECD) early in the 21st century. The study seeks to examine the forces that galvanize the growth of energy use and carbon emissions, to assess the likely future levels of energy and CO{sub 2} in selected developing nations and to identify opportunities for restraining this growth. The purpose of this report is to provide the quantitative information needed to develop effective policy options, not to identify the options themselves. These individual studies were conducted fro Argentina, Brazil, Mexico and Venezuela in Latin America.

  7. Experiences in the design of CRA`s for erosion/corrosion control in the production facilities of eastern Venezuela oil fields

    SciTech Connect (OSTI)

    Romero, N.; Palacios, C.A.

    1997-08-01

    It is a well known fact that CRA`s are used in the oil industry as one way to control erosion/corrosion effects. Many fields in the eastern region of Venezuela are considered corrosive due to the presence of CO{sub 2} (5 to 20%), H{sub 2}S (up to 5 ppm), and water (50% water cut) contained in the produced hydrocarbons (condensated). For some areas, the hydrocarbon is accompanied by sand, making them erosive as well. These conditions and frequent failures experienced in the field, led to the use of CRA`s. For the wells, 13% Cr and bimetallic (carbon steel/13% Cr) tubing was used for 51 condensate wells containing 5 to 20% CO{sub 2}. For the surface equipment (valves, reducers, expanders and other types of fittings) tungsten carbide hard facing were used, for some of the valves, a epoxi-phenolic coating was used. This article describes the different design criteria used for the installation of the tubing, the logistics involved during field inspections and handling tips to avoid galling during workovers. It also, presents results from the bi-metallic tubing and the hard facings used for the surface equipment.

  8. Sedimentary evolution of the upper Cretaceous and late Oligocene sequences, and its relation to oil production, North Monagas area, Eastern Venezuela

    SciTech Connect (OSTI)

    Sambrano, J.; Rojas, B.; Rendon, J.; Chigne, R.; Maguregui, J.

    1996-08-01

    The most important oil reservoirs of the Eastern Venezuela Basin are located in the North Monagas Area. These reservoirs are contained within a 3500 ft Cretaceous to Late Oligocene sedimentary section. Daily production is rated at about 350 MBO and 1000 MMCFG. At this moment, these reservoirs are undergoing special studies, in order to establish enhanced recovery projects, for which heterogeneity definition is very important. The database consisted of log analyses of 136 wells, sedimentological and biostratigraphic interpretation of 10,200 ft of cores, and biostratigraphic interpretation of ditch samples from 13 wells. Sedimentary models, based on facies analyses and deltaic conceptual models of 31 separate genetic units were defined. The models allowed for the interpretation of paleoenvironments, sedimentary facies architecture, direction of sedimentation and depocenters. The preferred sediment orientation was determined to be West-East. In the Santa Barbara and Pirital reservoirs the Late Oligocene sediments are composed of fluvial deposits, and the Cretaceous sediments of estuarine deposits. In the Carito-Mulata reservoirs, the Late Oligocene sediments are composed of fluvial to marine deposits, and the Upper Cretaceous sediments of estuarine deposits. Possible preferred transmissibility pathways for fluid injection were described, providing a great support for the enhanced recovery phases of these reservoirs.

  9. Multimedia regulated chemicals

    SciTech Connect (OSTI)

    Lee, C.C.; Huffman, G.L.; Mao, Y.L.

    1999-10-01

    This article examines those chemicals that are listed in either environmental laws or regulations. Its objective is to help readers determine which laws regulate what types of chemicals and which types of chemicals are regulated by what laws. It is multimedia in scope, describing the various chemicals that are regulated in the different media (i.e., air, water, or land).

  10. Estrogen induced {beta}-1,4-galactosyltransferase 1 expression...

    Office of Scientific and Technical Information (OSTI)

    cancer MCF-7 cells Citation Details In-Document Search Title: Estrogen induced beta-1,4-galactosyltransferase 1 expression regulates proliferation of human breast cancer ...

  11. Load regulating expansion fixture

    DOE Patents [OSTI]

    Wagner, L.M.; Strum, M.J.

    1998-12-15

    A free standing self contained device for bonding ultra thin metallic films, such as 0.001 inch beryllium foils is disclosed. The device will regulate to a predetermined load for solid state bonding when heated to a bonding temperature. The device includes a load regulating feature, whereby the expansion stresses generated for bonding are regulated and self adjusting. The load regulator comprises a pair of friction isolators with a plurality of annealed copper members located therebetween. The device, with the load regulator, will adjust to and maintain a stress level needed to successfully and economically complete a leak tight bond without damaging thin foils or other delicate components. 1 fig.

  12. Load regulating expansion fixture

    DOE Patents [OSTI]

    Wagner, Lawrence M.; Strum, Michael J.

    1998-01-01

    A free standing self contained device for bonding ultra thin metallic films, such as 0.001 inch beryllium foils. The device will regulate to a predetermined load for solid state bonding when heated to a bonding temperature. The device includes a load regulating feature, whereby the expansion stresses generated for bonding are regulated and self adjusting. The load regulator comprises a pair of friction isolators with a plurality of annealed copper members located therebetween. The device, with the load regulator, will adjust to and maintain a stress level needed to successfully and economically complete a leak tight bond without damaging thin foils or other delicate components.

  13. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Unique Auxin Regulation Mechanism Discovered Print Wednesday, 29 August 2007 00:00 The plant hormone auxin regulates many plant growth ...

  14. Pressure reducing regulator

    DOE Patents [OSTI]

    Whitehead, John C. (Davis, CA); Dilgard, Lemoyne W. (Willits, CA)

    1995-01-01

    A pressure reducing regulator that controls its downstream or outlet pressure to a fixed fraction of its upstream or inlet pressure. The regulator includes a housing which may be of a titanium alloy, within which is located a seal or gasket at the outlet end which may be made of annealed copper, a rod, and piston, each of which may be made of high density graphite. The regulator is insensitive to temperature by virtue of being without a spring or gas sealed behind a diaphragm, and provides a reference for a system in which it is being used. The rod and piston of the regulator are constructed, for example, to have a 1/20 ratio such that when the downstream pressure is less than 1/20 of the upstream pressure the regulator opens and when the downstream pressure exceeds 1/20 of the upstream pressure the regulator closes.

  15. Pressure reducing regulator

    DOE Patents [OSTI]

    Whitehead, J.C.; Dilgard, L.W.

    1995-10-10

    A pressure reducing regulator that controls its downstream or outlet pressure to a fixed fraction of its upstream or inlet pressure is disclosed. The regulator includes a housing which may be of a titanium alloy, within which is located a seal or gasket at the outlet end which may be made of annealed copper, a rod, and piston, each of which may be made of high density graphite. The regulator is insensitive to temperature by virtue of being without a spring or gas sealed behind a diaphragm, and provides a reference for a system in which it is being used. The rod and piston of the regulator are constructed, for example, to have a 1/20 ratio such that when the downstream pressure is less than 1/20 of the upstream pressure the regulator opens and when the downstream pressure exceeds 1/20 of the upstream pressure the regulator closes. 10 figs.

  16. Resources for Utility Regulators

    SciTech Connect (OSTI)

    SEE Action

    2012-06-01

    Provides a summary of State and Local Energy Efficiency Action Network (SEE Action) information resources available to utility regulators, organized by topic.

  17. Policy and Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations Policy and Regulations map-1019833_960_720.jpg

  18. WIPP Documents - Federal Regulations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Federal Regulations 40 CFR Part 191 Environmental radiation protection standards for management and disposal of spent nuclear fuel, high-level and transuranic radioactive wastes. 40 CFR Part 194 Criteria for the certification and re-certification of the Waste Isolation Pilot Plant's compliance with the 40 CFR Part 191 disposal regulations.

  19. Of Refrigerators & Regulations

    Broader source: Energy.gov [DOE]

    Editor's Note: This entry has been cross-posted from The White House Blog. The President took a moment during his speech to put the debate over regulation in a different perspective.

  20. HIGH PRESSURE GAS REGULATOR

    DOE Patents [OSTI]

    Ramage, R.W.

    1962-05-01

    A gas regulator operating on the piston and feedback principle is described. The device is particularly suitable for the delicate regulation of high pressure, i.e., 10,000 psi and above, gas sources, as well as being perfectly adaptable for use on gas supplies as low as 50 psi. The piston is adjustably connected to a needle valve and the movement of the piston regulates the flow of gas from the needle valve. The gas output is obtained from the needle valve. Output pressure is sampled by a piston feedback means which, in turn, regulates the movement of the main piston. When the output is other than the desired value, the feedback system initiates movement of the main piston to allow the output pressure to be corrected or to remain constant. (AEC)

  1. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ...

  2. Reviving regulation - and antitrust

    SciTech Connect (OSTI)

    Shepherd, W.G.

    1994-06-01

    The ills of utility regulation have been overstated by interested players and at least some academics. One can have the best features of both competition and regulation by a paradigm that combines some regulation with stronger antitrust enforcement. It is now obvious that the success of deregulation actually depends crucially on whether antitrust policy is effective in preventing monopoly. In fact, antitrust policies have been extremely weak since 1980, under a deliberate Reagan administration withdrawal of enforcement. Where deregulation has led to market dominance, which antitrust cannot well cure, the policy changes of the 1980`s may have created costly and irreversible mistakes in a number of major sectors. Yet fast-paced events are currently creating a frenzy of even more deregulation, supposedly to fit new {open_quotes}revolutions{close_quotes} in technology and the impacts of large mergers. Instead it is time for particular caution in electricity.

  3. Use of geostatistic techniques to describe a reservoir to be submitted into a secondary recovery process field case: {open_quotes}Eocene B-Inferior/VLG-3659, Ceuta, Venezuela{close_quotes}

    SciTech Connect (OSTI)

    Hernandez, T.; Poquioma, W.

    1997-08-01

    This study presents the results of an integrated reservoir study of the Eocene B-Inferior/VLG-3659, Area 7, Ceuta filed. This field located in the Maracaibo Lake in the western side of Venezuela. The objective was to evaluating the feasibility to implement a secondary recovery project by means of water flooding. Core information was used for this study (194 ft), PVT analysis, RFI, build-up and statistic`s pressure analysis, modem logs and production history data. Using geostatistical techniques (Kriging) it was defined a low uncertainty geological model that was validated by means of a black oil simulator (Eclipse). The results showed a good comparison of historical pressure of the reservoir against those obtained from the model, without the need of {open_quotes}history matching{close_quotes}. It means without modifying neither the initial rock properties nor reservoir fluids. The results of this study recommended drilling in two new locations, also the reactivation of four producing wells and water flooding under peripherical array by means of four injection wells, with the recovery of an additional 30.2 MMSTB. The economical evaluation shows an internal return rate of 31.4%.

  4. ELECTRON EMISSION REGULATING MEANS

    DOE Patents [OSTI]

    Brenholdt, I.R.

    1957-11-19

    >An electronic regulating system is described for controlling the electron emission of a cathode, for example, the cathode in a mass spectrometer. The system incorporates a transformer having a first secondary winding for the above-mentioned cathode and a second secondary winding for the above-mentioned cathode and a second secondary winding load by grid controlled vacuum tubes. A portion of the electron current emitted by the cathode is passed through a network which develops a feedback signal. The system arrangement is completed by using the feedback signal to control the vacuum tubes in the second secondary winding through a regulator tube. When a change in cathode emission occurs, the feedback signal acts to correct this change by adjusting the load on the transformer.

  5. Regulation of Meiotic Recombination

    SciTech Connect (OSTI)

    Gregory p. Copenhaver

    2011-11-09

    Meiotic recombination results in the heritable rearrangement of DNA, primarily through reciprocal exchange between homologous chromosome or gene conversion. In plants these events are critical for ensuring proper chromosome segregation, facilitating DNA repair and providing a basis for genetic diversity. Understanding this fundamental biological mechanism will directly facilitate trait mapping, conventional plant breeding, and development of genetic engineering techniques that will help support the responsible production and conversion of renewable resources for fuels, chemicals, and the conservation of energy (1-3). Substantial progress has been made in understanding the basal recombination machinery, much of which is conserved in organisms as diverse as yeast, plants and mammals (4, 5). Significantly less is known about the factors that regulate how often and where that basal machinery acts on higher eukaryotic chromosomes. One important mechanism for regulating the frequency and distribution of meiotic recombination is crossover interference - or the ability of one recombination event to influence nearby events. The MUS81 gene is thought to play an important role in regulating the influence of interference on crossing over. The immediate goals of this project are to use reverse genetics to identify mutants in two putative MUS81 homologs in the model plant Arabidopsis thaliana, characterize those mutants and initiate a novel forward genetic screen for additional regulators of meiotic recombination. The long-term goal of the project is to understand how meiotic recombination is regulated in higher eukaryotes with an emphasis on the molecular basis of crossover interference. The ability to monitor recombination in all four meiotic products (tetrad analysis) has been a powerful tool in the arsenal of yeast geneticists. Previously, the qrt mutant of Arabidopsis, which causes the four pollen products of male meiosis to remain attached, was developed as a facile system

  6. Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems

    Broader source: Energy.gov [DOE]

    This Protocol is a living guidance document for geothermal developers, public officials, regulators and the general public that provides a set of general guidelines detailing useful steps to evaluate and manage the effects of induced seismicity related to EGS projects.

  7. Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems

    SciTech Connect (OSTI)

    Majer, Ernie; Nelson, James; Robertson-Tait, Ann; Savy, Jean; Wong, Ivan

    2012-01-01

    This Protocol is a living guidance document for geothermal developers, public officials, regulators and the general public that provides a set of general guidelines detailing useful steps to evaluate and manage the effects of induced seismicity related to EGS projects.

  8. Interrelationship of Program Regulations and Financial Assistance Regulations

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1980-04-24

    The purpose of this Order is to set forth the interrelationship between all program regulations which will result in assistance awards (Program Regulations) and the Department of Energy Assistance Regulations (DOE-AR, 10 CRF Part 600), including procedures for exceptions, deviations or waivers.

  9. Isolated yeast promoter sequence and a method of regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway; Skeen, Rodney S.; Hooker, Brian S.; Anderson, Daniel B.

    2005-05-31

    The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  10. Self-regulating valve

    DOE Patents [OSTI]

    Humphreys, D.A.

    1982-07-20

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  11. Weatherization Rules and Regulations Resources

    Broader source: Energy.gov [DOE]

    Federal regulations provide the framework for the Weatherization Assistance Program. These rules and regulations give state and local weatherization programs guidelines to provide the energy efficiency improvements to low-income dwellings.

  12. Regulators, Boards, Councils - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Regulators, Boards, Councils Hanford Advisory Board Hanford Natural Resource Trustee Council Environmental Protection Agency Washington State Department of Ecology Defense...

  13. Temperature controlled high voltage regulator

    DOE Patents [OSTI]

    Chiaro, Jr., Peter J.; Schulze, Gerald K.

    2004-04-20

    A temperature controlled high voltage regulator for automatically adjusting the high voltage applied to a radiation detector is described. The regulator is a solid state device that is independent of the attached radiation detector, enabling the regulator to be used by various models of radiation detectors, such as gas flow proportional radiation detectors.

  14. Cascaded'' pilot regulators help reduce LPG loss in hot weather

    SciTech Connect (OSTI)

    Not Available

    1994-08-08

    Fina Oil and Chemical Co. and Fisher Controls International used engineering resourcefulness to overcome heat-induced product loss from LPG storage bullets at Fina's Port Arthur, Tex., refinery. Fina had installed Fisher's Easy Joe 399A-6365, a pilot-operated, back-pressure-type regulator, on its LPG storage facility in 1991 as part of a fuel products modernization project. The regulators helped control the accumulation of noncondensible vapors, which collect in the storage bullets above the LPG. But summer heat extremes and surges in the tanks and lines made it possible for the operating pressure to increase so that the safety relief valve was activated before the pilot regulator was able to stabilize the pressure. The installation of pilot-type regulators, in cascaded, or series, formation, reduced product venting through relief valves.

  15. Protocol for Addressing Induced Seismicity Associated with Enhanced

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Geothermal Systems (EGS) | Department of Energy Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems (EGS) Protocol for Addressing Induced Seismicity Associated with Enhanced Geothermal Systems (EGS) This document is intended to assist industry and regulators identify important issues and parameters that may be necessary for the evaluation and mitigation of adverse effects of induced seismicity. egs-is-protocol-final-draft-20110531.pdf (218.87 KB) More

  16. Vermont Hazardous Waste Management Regulations | Open Energy...

    Open Energy Info (EERE)

    Hazardous Waste Management Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Vermont Hazardous Waste Management...

  17. ELECTRICAL REGULATING APPARATUS INCLUDING AN IONIC CURRENT REGULATOR

    DOE Patents [OSTI]

    Brackney, H.W.

    1958-08-12

    An apparatus is described for regulating the operation of an electromagmetic charged particle separator lt consists of an electrical circuit for innproving the regulation of the accelerating voltage of a calutron when the ionic current regulator control means is disconnected. The novel circuit arrangement connects the input of the ionic current regulator to a voltage divider. in association with a second voltage regulatora to furnish an accelerating voltage output which remains constant at a mean value instead of zero as has been the practice.

  18. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Unique Auxin Regulation Mechanism Discovered Print Wednesday, 29 August 2007 00:00 The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction

  19. Collegiate Wind Competition Rules and Regulations | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Collegiate Wind Competition Rules and Regulations Collegiate Wind Competition Rules and Regulations The competition challenges interdisciplinary teams of undergraduate students ...

  20. Litigation, Regulation and Enforcement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Litigation, Regulation and Enforcement Litigation, Regulation and Enforcement Litigation, Regulation and Enforcement Offices of the Deputy General Counsel for Litigation, Regulation and Enforcement Office of the Assistant General Counsel for Litigation (GC-31) Office of the Assistant General Counsel for Enforcement (GC-32) Office of the Assistant General Counsel for Legislation, Regulation and Energy Efficiency (GC-33) Litigation, Regulation and Enforcement Litigation Enforcement Legislation,

  1. Street sweeping and stormwater regulations

    SciTech Connect (OSTI)

    Not Available

    1993-10-01

    This article examines the role of street sweeping in meeting the requirements of the Clean Water Act stormwater regulations. The article identifies those industrial and municipal activities which are covered by the regulations and cites frequent sweeping of site surfaces for industry and street sweeping for municipalities as an integral part of compliance plans.

  2. RNA-guided transcriptional regulation

    DOE Patents [OSTI]

    Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

    2016-02-23

    Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

  3. Insight in the multilevel regulation of NER

    SciTech Connect (OSTI)

    Dijk, Madelon; Typas, Dimitris; Mullenders, Leon; Pines, Alex

    2014-11-15

    Nucleotide excision repair (NER) is a key component of the DNA damage response (DDR) and it is essential to safeguard genome integrity against genotoxic insults. The regulation of NER is primarily mediated by protein post-translational modifications (PTMs). The NER machinery removes a wide spectrum of DNA helix distorting lesions, including those induced by solar radiation, through two sub-pathways: global genome nucleotide excision repair (GG-NER) and transcription coupled nucleotide excision repair (TC-NER). Severe clinical consequences associated with inherited NER defects, including premature ageing, neurodegeneration and extreme cancer-susceptibility, underscore the biological relevance of NER. In the last two decades most of the core NER machinery has been elaborately described, shifting attention to molecular mechanisms that either facilitate NER in the context of chromatin or promote the timely and accurate interplay between NER factors and various post-translational modifications. In this review, we summarize and discuss the latest findings in NER. In particular, we focus on emerging factors and novel molecular mechanisms by which NER is regulated.

  4. Down-regulation of PERK enhances resistance to ionizing radiation

    SciTech Connect (OSTI)

    Oommen, Deepu Prise, Kevin M.

    2013-11-08

    Highlights: •PERK enhances the sensitivity of cancer cells to ionizing radiation. •Down-regulation of PERK results in enhanced DNA repair. •Ionizing radiation-induced apoptosis is inhibited in PERK-down regulated cancer cells. -- Abstract: Although, ionizing radiation (IR) has been implicated to cause stress in endoplasmic reticulum (ER), how ER stress signaling and major ER stress sensors modulate cellular response to IR is unclear. Protein kinase RNA-like endoplasmic reticulum kinase (PERK) is an ER transmembrane protein which initiates unfolded protein response (UPR) or ER stress signaling when ER homeostasis is disturbed. Here, we report that down-regulation of PERK resulted in increased clonogenic survival, enhanced DNA repair and reduced apoptosis in irradiated cancer cells. Our study demonstrated that PERK has a role in sensitizing cancer cells to IR.

  5. Alternative Fuels Data Center: Conversion Regulations

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Conversion Regulations to someone by E-mail Share Alternative Fuels Data Center: Conversion Regulations on Facebook Tweet about Alternative Fuels Data Center: Conversion Regulations on Twitter Bookmark Alternative Fuels Data Center: Conversion Regulations on Google Bookmark Alternative Fuels Data Center: Conversion Regulations on Delicious Rank Alternative Fuels Data Center: Conversion Regulations on Digg Find More places to share Alternative Fuels Data Center: Conversion Regulations on

  6. APPARATUS FOR REGULATING HIGH VOLTAGE

    DOE Patents [OSTI]

    Morrison, K.G.

    1951-03-20

    This patent describes a high-voltage regulator of the r-f type wherein the modulation of the r-f voltage is accomplished at a high level, resulting in good stabilization over a large range of load conditions.

  7. Environmental Regulations | Open Energy Information

    Open Energy Info (EERE)

    Regulations Jump to: navigation, search To do: add table This article is a stub. You can help OpenEI by expanding it. Retrieved from "http:en.openei.orgwindex.php?titleEnviron...

  8. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  9. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  10. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  11. Unique Auxin Regulation Mechanism Discovered

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unique Auxin Regulation Mechanism Discovered Print The plant hormone auxin regulates many plant growth and development processes, including shoot growth, root branching, fruit ripening, tropisms, and flowering. But how such a simple molecule elicits such a variety of cellular responses has been a mystery. An important breakthrough came in 2005, wh en a conserved plant protein known as TIR1 (part of a protein destruction machinery system) was identified as a receptor for auxin. Now, an

  12. Federal Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Federal Regulations Federal Regulations NOTE: Adobe Acrobat Reader may be necessary to view PDF documents listed below. Accessibility Section 508 of the Rehabilitation Act - Electronic and Information Technology Accessibility Standards Section 504 of the Rehabilitation Act - Nondiscrimination Under Federal Grants and Programs Federal Records Act Freedom of Information Act (as amended) Freedom of Information Act Updates Privacy Act of 1974 (as amended) Privacy Act Overview Cyber Security Atomic

  13. Post regulation circuit with energy storage

    DOE Patents [OSTI]

    Ball, Don G.; Birx, Daniel L.; Cook, Edward G.

    1992-01-01

    A charge regulation circuit provides regulation of an unregulated voltage supply and provides energy storage. The charge regulation circuit according to the present invention provides energy storage without unnecessary dissipation of energy through a resistor as in prior art approaches.

  14. Output-Based Regulations: A Handbook for Air Regulators (U.S...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004 Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004 The U.S. ...

  15. Progress toward risk informed regulation

    SciTech Connect (OSTI)

    Rogers, K.C.

    1997-01-01

    For the last several years, the NRC, with encouragement from the industry, has been moving in the direction of risk informed regulation. This is consistent with the regulatory principle of efficiency, formally adopted by the Nuclear Regulatory Commission in 1991, which requires that regulatory activities be consistent with the degree of risk reduction they achieve. Probabilistic risk analysis has become the tool of choice for selecting the best of several alternatives. Closely related to risk informed regulation is the development of performance based rules. Such rules focus on the end result to be achieved. They do not specify the process, but instead establish the goals to be reached and how the achievement of those goals is to be judged. The inspection and enforcement activity is based on whether or not the goals have been met. The author goes on to offer comments on the history of the development of this process and its probable development in the future. He also addresses some issues which must be resolved or at least acknowledged. The success of risk informed regulation ultimately depends on having sufficiently reliable data to allow quantification of regulatory alternatives in terms of relative risk. Perhaps the area of human reliability and organizational performance has the greatest potential for improvement in reactor safety. The ability to model human performance is significantly less developed that the ability to model mechanical or electrical systems. The move toward risk informed, performance based regulation provides an unusual, perhaps unique, opportunity to establish a more rational, more effective basis for regulation.

  16. International Electricity Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation International Electricity Regulation U.S. trade in electric energy with Canada and Mexico is rising, bringing economic and reliability benefits to the United States and ...

  17. Searchable Electronic Department of Energy Acquisition Regulation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Searchable Electronic Department of Energy Acquisition Regulation Searchable Electronic Department of Energy Acquisition Regulation Updated July 2, 2013. The EDEAR is current ...

  18. Department of Energy Acquisition Regulation (DEAR) Technical...

    Broader source: Energy.gov (indexed) [DOE]

    of Energy Acquisition Regulation (DEAR) Technical Amendment-Final Rule (172.78 KB) More Documents & Publications Department of Energy Acquisition Regulation (DEAR) Technical ...

  19. Fish Habitat Regulations | Open Energy Information

    Open Energy Info (EERE)

    Fish Habitat Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Fish Habitat Regulations Author Alaska Department of Fish & Game Published...

  20. Alaska ADEC Wetlands Regulation | Open Energy Information

    Open Energy Info (EERE)

    Wetlands Regulation Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Alaska ADEC Wetlands Regulation Author Alaska Division of Water Published Alaska...

  1. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, Charlton; Subbaraman, Maria R.

    1989-01-01

    An improvement in an inducer for a pump wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs.

  2. Cavitation-resistant inducer

    DOE Patents [OSTI]

    Dunn, C.; Subbaraman, M.R.

    1989-06-13

    An improvement in an inducer for a pump is disclosed wherein the inducer includes a hub, a plurality of radially extending substantially helical blades and a wall member extending about and encompassing an outer periphery of the blades. The improvement comprises forming adjacent pairs of blades and the hub to provide a substantially rectangular cross-sectional flow area which cross-sectional flow area decreases from the inlet end of the inducer to a discharge end of the inducer, resulting in increased inducer efficiency improved suction performance, reduced susceptibility to cavitation, reduced susceptibility to hub separation and reduced fabrication costs. 11 figs.

  3. ISOs: The new antitrust regulators?

    SciTech Connect (OSTI)

    Raskin, D.B.

    1998-04-01

    Fear of seller market power in emerging electricity markets has led regulators to sanction use of independent system operators as private market police. A more restrained approach is likely to yield better results without the chilling effects of private regulation. This new industry regulatory paradigm has received little critical attention to date. This is unfortunate because ISO antitrust regulation raises serious legal and policy concerns. The California and New England Power Pool (NEPOOL) plans are quite intrusive. They require the ISO to make difficult distinctions between acceptable and unacceptable market behavior. They create considerable risk that desirable competitive behavior will be chilled and that market participants will incur significant explicit and implicit costs to meet regulatory requirements.

  4. Mara Field, a unique giant in Venezuela

    SciTech Connect (OSTI)

    Young, G.A. )

    1993-02-01

    The Mara field is located in Venzuela, 45 km northwest of Maracaibo, on the Mara-La Paz anticlinal trend. Discovered in 1945 by the Caribbean Petroleum Co. (Shell group), the field has produced 407 MMB as of 1991 and has remaining proven reserves of 60 MMB, and probable and possible reserves of 58 MMB, for an ultimate potential recovery of 525 MMB. In addition to being a giant field, Mara is also unique in that it produces from fractures igneous basement rocks as well as from fractured Cretaceous limestones, which are the source rocks of the region, and Paleocene/Eocene sandstone and carbonate reservoirs. The sedimentary stratigraphic section comprises beds ranging from early Cretaceous to middle Eocene, which suffered considerable erosion, and overlying Plio-Pleistocene sediments, all of which were involved in the latest strong deformation. The structure of the field is complex; a main thrust zone (consisting of numerous individual faults) borders the northwest flank of the elongated anticline and an opposing minor thrust zone cuts the southeast flank, forming a thrusted horst. Oblique transverse faults also cut the structure. By studying the patterns of cumulative production and lost circulation, it was possible to derive relationships between the accumulations of oil and the faulting and conceptual patterns of related fracturing in the different types of reservoir rocks. The study indicates that one can prognosticate the more prospective drilling locations on this or similar structures involving basement and limestone. It is felt that this information may be applicable to other plays in other regions.

  5. Venezuela: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    in the country has fallen, while domestic consumption has risen, causing a decline in net oil exports. EIA estimates the Venezuelan net exports fell again in 2010 to 1.59...

  6. Chapter 2 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    2 of the Wyoming Public Service Commission Regulations: General Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  7. Rainwater Harvesting Regulations Map | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations Map Rainwater Harvesting Regulations Map Rainwater collection is currently regulated by individual states. There was no centralized information source on state-level regulations on rainwater harvesting maintained by a federal agency. To fill this information gap, the Federal Energy Management Program compiled state-level information and provided it in this map tool. How to Use the Map This map gives federal agencies key information on how rainwater is regulated across the U.S. to

  8. Negative feedback regulation of Homer 1a on norepinephrine-dependent cardiac hypertrophy

    SciTech Connect (OSTI)

    Chiarello, Carmelina; Bortoloso, Elena; Carpi, Andrea; Furlan, Sandra; Volpe, Pompeo

    2013-07-15

    Homers are scaffolding proteins that modulate diverse cell functions being able to assemble signalling complexes. In this study, the presence, sub-cellular distribution and function of Homer 1 was investigated. Homer 1a and Homer 1b/c are constitutively expressed in cardiac muscle of both mouse and rat and in HL-1 cells, a cardiac cell line. As judged by confocal immunofluorescence microscopy, Homer 1a displays sarcomeric and peri-nuclear localization. In cardiomyocytes and cultured HL-1 cells, the hypertrophic agonist norepinephrine (NE) induces α{sub 1}-adrenergic specific Homer 1a over-expression, with a two-to-three-fold increase within 1 h, and no up-regulation of Homer 1b/c, as judged by Western blot and qPCR. In HL-1 cells, plasmid-driven over-expression of Homer 1a partially antagonizes activation of ERK phosphorylation and ANF up-regulation, two well-established, early markers of hypertrophy. At the morphometric level, NE-induced increase of cell size is likewise and partially counteracted by exogenous Homer 1a. Under the same experimental conditions, Homer 1b/c does not have any effect on ANF up-regulation nor on cell hypertrophy. Thus, Homer 1a up-regulation is associated to early stages of cardiac hypertrophy and appears to play a negative feedback regulation on molecular transducers of hypertrophy. -- Highlights: • Homer 1a is constitutively expressed in cardiac tissue. • In HL-1 cells, norepinephrine activates signaling pathways leading to hypertrophy. • Homer 1a up-regulation is an early event of norepinephrine-induced hypertrophy. • Homer 1a plays a negative feedback regulation modulating pathological hypertrophy. • Over-expression of Homer 1a per se does not induce hypertrophy.

  9. Renewable energy and utility regulation

    SciTech Connect (OSTI)

    Not Available

    1991-04-10

    This report summarizes the results of a joint project on renewable energy of the National Association of Regulatory Utility Commissioners (NARUC) and the US DOE. NARUC'S Task Force on Renewable Energy conducted a review of the current state of renewable energy technologies to evaluate their potential and extract key policy lessons from experience already gained in deployment of these technologies in numerous states. The main focus of this effort has been to clarify how utility regulators affect the development of renewable energy resources. The goal of the project was twofold: (1) identify the factors that have led to success or failure or renewable energy technologies in various energy markets, and (2) to develop an agenda on renewable energy and utility regulation for NARUC and the DOE. This report consists of three sections: renewable energy contributions, costs and potential; factors affecting development of renewable energy resources; and a renewable energy agenda for NARUC.

  10. Renewable energy and utility regulation

    SciTech Connect (OSTI)

    Not Available

    1991-04-10

    This report summarizes the results of a joint project on renewable energy of the National Association of Regulatory Utility Commissioners (NARUC) and the US DOE. NARUC`S Task Force on Renewable Energy conducted a review of the current state of renewable energy technologies to evaluate their potential and extract key policy lessons from experience already gained in deployment of these technologies in numerous states. The main focus of this effort has been to clarify how utility regulators affect the development of renewable energy resources. The goal of the project was twofold: (1) identify the factors that have led to success or failure or renewable energy technologies in various energy markets, and (2) to develop an agenda on renewable energy and utility regulation for NARUC and the DOE. This report consists of three sections: renewable energy contributions, costs and potential; factors affecting development of renewable energy resources; and a renewable energy agenda for NARUC.

  11. Photomultiplier tube gain regulating system

    DOE Patents [OSTI]

    Johnson, Wayne F.

    1976-01-01

    This invention relates to an improved system for regulating the gain of a photomultiplier tube, and was designed for use with the photomultiplier tubes of a GeMSAEC fast analyzers. It has the following advantages over the prior system: noise is virtually eliminated; sample analysis can begin after 3 to 4 revolutions of the rotor; fluorescent and light scattering solutions can be used as a reference; and the reference solution can be in any cuvette on the rotor.

  12. Frequency regulator for synchronous generators

    DOE Patents [OSTI]

    Karlicek, R.F.

    1982-08-10

    The present invention is directed to a novel frequency regulator which controls a generator output frequency for variations in both the input power to the generator and the power supplied to an uncontrolled external load. The present invention further includes over current and current balance protection devices which are relatively inexpensive to manufacture, which may be encapsulated to provide protection from the operating environment and which respond more quickly than previously known electromechanical devices. 11 figs.

  13. GP-8 LAWS AND REGULATIONS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    40-DOP (5-2011) Supersedes (7-2010) issue DECLARATION of OCCUPATIONAL MEDICINE PROVIDER GENERAL INFORMATION PURPOSE All Contractors and their lower-tier subcontractors must comply with the Department of Energy's Worker Safety and Health Program regulation, 10 CFR 851, "Worker Safety and Health Program (WSHP). The WSHP enforces worker safety and health requirements including, but not limited to, standards of the Occupational Safety and Health Administration (OSHA), American National

  14. Frequency regulator for synchronous generators

    DOE Patents [OSTI]

    Karlicek, Robert F.

    1982-01-01

    The present invention is directed to a novel frequency regulator which controls a generator output frequency for variations in both the input power to the generator and the power supplied to an uncontrolled external load. The present invention further includes over current and current balance protection devices which are relatively inexpensive to manufacture, which may be encapsulated to provide protection from the operating environment and which respond more quickly than previously known electromechanical devices.

  15. Improving Regulation and Regulatory Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Alton Strategic Environmental Group New Port Richey, FL charles.alton@earthlink.net April 4, 2011 Daniel Cohen, Assistant General Counsel Legislation, Regulation, and Energy Efficiency Office of the General Counsel U.S. Department of Energy 1000 Independence Avenue, SW Washington, DC 20585 Dear Mr. Cohen: I have reviewed the Request For Information regarding Reducing Regulatory Reform issued February 3, 2011 (Federal Register /Vol. 76, No. 23 /Thursday, February 3, 2011 /Notices). In the

  16. NRC - regulator of nuclear safety

    SciTech Connect (OSTI)

    1997-05-01

    The U.S. Nuclear Regulatory Commission (NRC) was formed in 1975 to regulate the various commercial and institutional uses of nuclear energy, including nuclear power plants. The agency succeeded the Atomic Energy Commission, which previously had responsibility for both developing and regulating nuclear activities. Federal research and development work for all energy sources, as well as nuclear weapons production, is now conducted by the U.S. Department of Energy. Under its responsibility to protect public health and safety, the NRC has three principal regulatory functions: (1) establish standards and regulations, (2) issue licenses for nuclear facilities and users of nuclear materials, and (3) inspect facilities and users of nuclear materials to ensure compliance with the requirements. These regulatory functions relate to both nuclear power plants and to other uses of nuclear materials - like nuclear medicine programs at hospitals, academic activities at educational institutions, research work, and such industrial applications as gauges and testing equipment. The NRC places a high priority on keeping the public informed of its work. The agency recognizes the interest of citizens in what it does through such activities as maintaining public document rooms across the country and holding public hearings, public meetings in local areas, and discussions with individuals and organizations.

  17. Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

    DOE Patents [OSTI]

    Gao, Johnway [Richland, WA; Skeen, Rodney S [Pendleton, OR; Hooker, Brian S [Kennewick, WA; Anderson, Daniel B [Pasco, WA

    2003-04-01

    The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

  18. Surface mine regulations complicate reclamation

    SciTech Connect (OSTI)

    Seltz-Patrash, A.

    1980-09-01

    The Surface Mining Control and Reclamation Act of 1977 is a landmark environmental law intended to protect U.S. lands from stripmining effects. However, coal mine operators claim that some SMCRA regulations are misguidedcosting time and money, but yielding no substantial environmental benefit. Unlike other environmental acts, SMCRA details specifically the goals of reclamation and the methods that must be implemented to meet these goals. Coal industry representatives believe that this discourages innovation, promotes inefficiency by ignoring regional differences among sites, and results in unnecessary expense to the industry. Reclamation practices and progress among western coal mining companies are evaluated. (1 map, 5 photos)

  19. Colorado Code of Regulations 4 CCR 723-3, Rules Regulating Electric...

    Open Energy Info (EERE)

    Colorado Code of Regulations 4 CCR 723-3, Rules Regulating Electric Utilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  20. Code of Colorado Regulations 4 CCR 723-3, Rules Regulating Electric...

    Open Energy Info (EERE)

    Code of Colorado Regulations 4 CCR 723-3, Rules Regulating Electric Utilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  1. Output-Based Regulations: A Handbook for Air Regulators (U.S. EPA), August 2004

    Office of Energy Efficiency and Renewable Energy (EERE)

    Handbook providing practical information to help regulators decide if they want to use output-based regulations and explains how to develop an output-based emission standard

  2. Clean Water Act and Regulations (EPA)

    Broader source: Energy.gov [DOE]

    The Clean Water Act (CWA; 33 U.S.C. §1251 et seq.) establishes the basic structure for regulating discharges of pollutants into the waters of the United States and regulating quality standards for surface waters.

  3. Categorical Exclusion Determinations: Natural Gas Regulation | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Natural Gas Regulation Categorical Exclusion Determinations: Natural Gas Regulation Categorical Exclusion Determinations issued by Natural Gas Regulation. DOCUMENTS AVAILABLE FOR DOWNLOAD May 25, 2016 CX-200007 Categorical Exclusion Determination Cheniere Marketing, LLC CX(s) Applied: B5.7 Date: 05/25/2016 Location(s): Texas Office(s): Fossil Energy, Natural Gas Regulation May 18, 2016 CX-200008 Categorical Exclusion Determination Flint Hills Resources, LP CX(s) Applied: B5.7 Date:

  4. certification, compliance and enforcement regulations for Commercial

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Refrigeration Equipment (CRE) | Department of Energy certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) The current certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) "simply makes no sense". The regulations define the basic model as any product that has a different energy use or efficiency

  5. Department of Energy Acquisition Regulation (DEAR) Technical

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Amendment-Final Rule | Department of Energy Technical Amendment-Final Rule Department of Energy Acquisition Regulation (DEAR) Technical Amendment-Final Rule PF2009-66.pdf (171.29 KB) PF2009-66a - Attachment-Department of Energy Acquisition Regulation (DEAR) Technical Amendment-Final Rule (172.78 KB) More Documents & Publications Department of Energy Acquisition Regulation (DEAR) Technical Amendment-Final Rule Department of Energy Acquisition Regulation (DEAR) Final Rule for Subchapters

  6. Investment and regulation: the Dutch experience

    SciTech Connect (OSTI)

    Haffner, Robert; Helmer, Dorine; van Til, Harry

    2010-06-15

    Theoretical studies on the relationship between incentive regulation and investment in network industries generally point out that incentive regulation has a negative impact on investment. However, empirical evidence in this area is scarce. An analysis suggests that in the Dutch electricity and gas networks since 2001, incentive regulation has ensured a more rational and professional approach towards investments, with investment levels coming down somewhat at the start of the regulation but picking up later on. (author)

  7. Regulations for beneficial use changing nationwide

    SciTech Connect (OSTI)

    Goss, D.

    2008-07-01

    Regulations on the use of coal combustion products (CCPs) that are being considered by various US states are reviewed.

  8. Environmental Laws and Regulations | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Record Files Natural Resource Damage Assessments Environmental Laws and Regulations More Documents & Publications Paducah Public Involvement Plan Portsmouth Removal Actions...

  9. certification, compliance and enforcement regulations for Commercial...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon certification, compliance and enforcement regulations for Commercial Refrigeration Equipment (CRE) More Documents & Publications Regulatory Burden RFI Executive Order ...

  10. Reciprocal regulation of LXRα activity by ASXL1 and ASXL2 in lipogenesis

    SciTech Connect (OSTI)

    Park, Ui-Hyun; Seong, Mi-ran; Kim, Eun-Joo; Hur, Wonhee; Kim, Sung Woo; Yoon, Seung Kew; Um, Soo-Jong

    2014-01-10

    Highlights: •ASXL1 and ASXL2 directly interact with ligand-bound LXRα. •Ligand-induced LXRα activity is repressed by ASXL1 and activated by ASXL2. •ASXL1 and ASXL2 bind to the LXRE of the LXRα target promoter. •ASXL1 and ASXL2 reciprocally regulate lipogenesis in liver cells. -- Abstract: Liver X receptor alpha (LXRα), a member of the nuclear receptor superfamily, plays a pivotal role in hepatic cholesterol and lipid metabolism, regulating the expression of genes associated with hepatic lipogenesis. The additional sex comb-like (ASXL) family was postulated to regulate chromatin function. Here, we investigate the roles of ASXL1 and ASXL2 in regulating LXRα activity. We found that ASXL1 suppressed ligand-induced LXRα transcriptional activity, whereas ASXL2 increased LXRα activity through direct interaction in the presence of the ligand. Chromatin immunoprecipitation (ChIP) assays showed ligand-dependent recruitment of ASXLs to ABCA1 promoters, like LXRα. Knockdown studies indicated that ASXL1 inhibits, while ASXL2 increases, lipid accumulation in H4IIE cells, similar to their roles in transcriptional regulation. We also found that ASXL1 expression increases under fasting conditions, and decreases in insulin-treated H4IIE cells and the livers of high-fat diet-fed mice. Overall, these results support the reciprocal role of the ASXL family in lipid homeostasis through the opposite regulation of LXRα.

  11. Physiology and Regulation of Calcium Channels in Stomatal Guard Cells

    SciTech Connect (OSTI)

    Schroeder, Julian I.

    2007-05-02

    Stomatal pores in the epidermis of leaves regulate the diffusion of CO2 into leaves for photosynthetic carbon fixation and control water loss of plants during drought periods. Guard cells sense CO2, water status, light and other environmental conditions to regulate stomatal apertures for optimization of CO2 intake and plant growth under drought stress. The cytosolic second messenger calcium contributes to stomatal movements by transducing signals and regulating ion channels in guard cells. Studies suggest that both plasma membrane Ca2+ influx channels and vacuolar/organellar Ca2+ release channels contribute to ABA-induced Ca2+ elevations in guard cells. Recent research in the P.I.'s laboratory has led to identification of a novel major cation-selective Ca2+-permeable influx channel (Ica) in the plasma membrane of Arabidopsis guard cells. These advances will allow detailed characterization of Ica plasma membrane Ca2+ influx channels in guard cells. The long term goal of this research project is to gain a first detailed characterization of these novel plasma membrane Ca2+-permeable channel currents in Arabidopsis guard cells. The proposed research will investigate the hypothesis that Ica represents an important Ca2+ influx pathway for ABA and CO2 signal transduction in Arabidopsis guard cells. These studies will lead to elucidation of key signal transduction mechanisms by which plants balance CO2 influx into leaves and transpirational water loss and may contribute to future strategies for manipulating gas exchange for improved growth of crop plants and for biomass production.

  12. Final Report for Regulation of Embryonic Development in Higher Plants

    SciTech Connect (OSTI)

    Harada, John J.

    2013-10-22

    The overall goal of the project was to define the cellular processes that underlie embryo development in plants at a mechanistic level. Our studies focused on a critical transcriptional regulator, Arabidopsis LEAFY COTYLEDON (LEC1), that is necessary and sufficient to induce processes required for embryo development. Because LEC1 regulates lipid accumulation during the maturation phase of embryo development, information about LEC1 may be useful in designing approaches to enhance biofuel production in plants. During the tenure of this project, we determined the molecular mechanisms by which LEC1 acts as a transcription factor in embryos. We also identified genes directly regulated by LEC1 and showed that many of these genes are involved in maturation processes. This information has been useful in dissecting the gene regulatory networks controlling embryo development. Finally, LEC1 is a novel isoform of a transcription factor that is conserved among eukaryotes, and LEC1 is active primarily in seeds. Therefore, we determined that the LEC1-type transcription factors first appeared in lycophytes during land plant evolution. Together, this study provides basic information that has implications for biofuel production.

  13. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  14. Chapter 9 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    9 of the Wyoming Public Service Commission Regulations: General Forms Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  15. Rules and Regulations of the Wyoming Industrial Siting Council...

    Open Energy Info (EERE)

    Document- RegulationRegulation: Rules and Regulations of the Wyoming Industrial Siting Council - Chapter 1Legal Abstract Industrial development information and siting rules and...

  16. Advisory Council on Historic Preservation - Section 106 Regulations...

    Open Energy Info (EERE)

    OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Advisory Council on Historic Preservation - Section 106 RegulationsLegal Abstract Section 106 requires...

  17. Rules and Regulations of the Wyoming Industrial Siting Council...

    Open Energy Info (EERE)

    Document- RegulationRegulation: Rules and Regulations of the Wyoming Industrial Siting Council - Chapter 2Legal Abstract Rules of practice and proceedures of the Industrial Siting...

  18. Rules and Regulations of the Industrial Siting Council, Chapter...

    Open Energy Info (EERE)

    Industrial Siting Council, Chapter 1 Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Rules and Regulations of the...

  19. Staff Listing - Office of Regulation and International Engagement...

    Office of Environmental Management (EM)

    Staff Listing - Office of Regulation and International Engagement Staff Listing - Office of Regulation and International Engagement Office of Regulation and International ...

  20. Title 27 California Code of Regulations: Environmental Protection...

    Open Energy Info (EERE)

    7 California Code of Regulations: Environmental Protection Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Title 27...

  1. WAC - 173-303 Dangerous Waste Regulations | Open Energy Information

    Open Energy Info (EERE)

    03 Dangerous Waste Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 173-303 Dangerous Waste...

  2. 2011 Air Quality Regulations Report | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Air Quality Regulations Report 2011 Air Quality Regulations Report PDF icon 2011 Air Quality Regulations Report120111.pdf More Documents & Publications 2011:...

  3. 2011: Air Quality Regulations Report | Department of Energy

    Office of Environmental Management (EM)

    : Air Quality Regulations Report 2011: Air Quality Regulations Report PDF icon 2011 Air Quality Regulations ReportA120911.pdf More Documents & Publications 2011...

  4. Regulations for Implementing the Procedural Provisions of NEPA...

    Energy Savers [EERE]

    Regulations for Implementing the Procedural Provisions of NEPA Regulations for Implementing the Procedural Provisions of NEPA PDF icon CEQ Regulations for Implementing the...

  5. 18 CFR 380 Regulations Implementing the National Environmental...

    Open Energy Info (EERE)

    380 Regulations Implementing the National Environmental Policy Act Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 18 CFR...

  6. Homeobox A7 stimulates breast cancer cell proliferation by up-regulating estrogen receptor-alpha

    SciTech Connect (OSTI)

    Zhang, Yu; Department of Obstetrics and Gynaecology, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4 ; Cheng, Jung-Chien; Huang, He-Feng; Leung, Peter C.K.

    2013-11-01

    Highlights: HOXA7 regulates MCF7 cell proliferation. HOXA7 up-regulates ER? expression. HOXA7 mediates estrogen-induced MCF7 cell proliferation. -- Abstract: Breast cancer is the most common hormone-dependent malignancy in women. Homeobox (HOX) transcription factors regulate many cellular functions, including cell migration, proliferation and differentiation. The aberrant expression of HOX genes has been reported to be associated with human reproductive cancers. Estradiol (E2) and its nuclear receptors, estrogen receptor (ER)-alpha and ER-beta, are known to play critical roles in the regulation of breast cancer cell growth. However, an understanding of the potential relationship between HOXA7 and ER in breast cancer cells is limited. In this study, our results demonstrate that knockdown of HOXA7 in MCF7 cells significantly decreased cell proliferation and ER? expression. In addition, HOXA7 knockdown attenuated E2-induced cell proliferation as well as progesterone receptor (PR) expression. The stimulatory effects of E2 on cell proliferation and PR expression were abolished by co-treatment with ICI 182780, a selective ER? antagonist. In contrast, overexpression of HOXA7 significantly stimulated cell proliferation and ER? expression. Moreover, E2-induced cell proliferation, as well as PR expression, was enhanced by the overexpression of HOXA7. Neither knockdown nor overexpression of HOXA7 affected the ER-beta levels. Our results demonstrate a novel mechanistic role for HOXA7 in modulating breast cancer cell proliferation via regulation of ER? expression. This finding contributes to our understanding of the role HOXA7 plays in regulating the proliferation of ER-positive cancer cells.

  7. EPA Regulation Compliance | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EPA Regulation Compliance EPA Regulation Compliance OE offers technical assistance on implementing the new and pending EPA air rules affecting the electric utility industry. Examples of typical assistance include technical information on cost and performance of the various power plant pollution retrofit control technologies; technical information on generation, demand-side or transmission alternatives for any replacement power needed for retiring generating units; and assistance to regulators

  8. Small Self-Regulating Fission Reactor System

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Small Self-Regulating Fission Reactor System ANTICIPATED IMPACT PATH FORWARD DESCRIPTION BACKGROUND & MOTIVATION INNOVATION A power system for special government applications Point of Contact: Patrick McClure, NEN-5, pmcclure@lanl.gov (505)667-9534 Small Self-Regulating Fission Reactor System A small self- regulating fission reactor made with U 235 . LANL and NASA with the support of NSTec performed a proof of concept test at the Nevada Test Site. Test apparatus - Nuclear material on left

  9. Differential regulation of HIF-1α and HIF-2α in neuroblastoma: Estrogen-related receptor alpha (ERRα) regulates HIF2A transcription and correlates to poor outcome

    SciTech Connect (OSTI)

    Hamidian, Arash; Stedingk, Kristoffer von; Munksgaard Thorén, Matilda; Mohlin, Sofie; Påhlman, Sven

    2015-06-05

    Hypoxia-inducible factors (HIFs) are differentially regulated in tumor cells. While the current paradigm supports post-translational regulation of the HIF-α subunits, we recently showed that hypoxic HIF-2α is also transcriptionally regulated via insulin-like growth factor (IGF)-II in the childhood tumor neuroblastoma. Here, we demonstrate that transcriptional regulation of HIF-2α seems to be restricted to neural cell-derived tumors, while HIF-1α is canonically regulated at the post-translational level uniformly across different tumor forms. Enhanced expression of HIF2A mRNA at hypoxia is due to de novo transcription rather than increased mRNA stability, and chemical stabilization of the HIF-α proteins at oxygen-rich conditions unexpectedly leads to increased HIF2A transcription. The enhanced HIF2A levels do not seem to be dependent on active HIF-1. Using a transcriptome array approach, we identified members of the Peroxisome proliferator-activated receptor gamma coactivator (PGC)/Estrogen-related receptor (ERR) complex families as potential regulators of HIF2A. Knockdown or inhibition of one of the members, ERRα, leads to decreased expression of HIF2A, and high expression of the ERRα gene ESRRA correlates with poor overall and progression-free survival in a clinical neuroblastoma material consisting of 88 tumors. Thus, targeting of ERRα and pathways regulating transcriptional HIF-2α are promising therapeutic avenues in neuroblastoma. - Highlights: • Transcriptional control of HIF-2α is restricted to neural cell-derived tumors. • Enhanced transcription of HIF2A is not due to increased mRNA stability. • Chemical stabilization of the HIF-α subunits leads to increased HIF2A transcription. • ERRα regulates HIF2A mRNA expression in neuroblastoma. • High expression of ESRRA correlates to poor outcome in neuroblastoma.

  10. Volume regulation by human lymphocytes. Role of calcium

    SciTech Connect (OSTI)

    Grinstein, S.; Dupre, A.; Rothstein, A.

    1982-05-01

    Human peripheral blood lymphocytes regulate their volumes in hypotonic solutions. In hypotonic media in which Na+ is the predominant cation, an initial swelling phase is followed by a regulatory volume decrease (RVD) associated with a net loss of cellular K+. In media in which K+ is the predominant cation, the rapid initial swelling is followed by a slower second swelling phase. /sup 86/Rb+ fluxes increased during RVD and returned to normal when the original volume was approximately regained. Effects similar to those induced by hypotonic stress could also be produced by raising the intracellular Ca++ level. In isotonic, Ca++-containing media cells were found to shrink upon addition of the Ca++ ionophore A23187 in K+-free media, but to swell in K+-rich media. Exposure to Ca++ plus A23187 also increased /sup 86/Rb+ fluxes. Quinine (75 microM), an inhibitor of the Ca++-activated K+ pathway in other systems blocked RVD, the associated K+ loss, and the increase in /sup 86/Rb+ efflux. Quinine also inhibited the volume changes and the increased /sup 86/Rb fluxes induced by Ca++ plus ionophore. The calmodulin inhibitors trifluoperazine, pimozide and chlorpromazine blocked RVD as well as Ca++ plus A23187-induced volume changes. Trifluoperazine also prevented the increase in /sup 86/Rb+ fluxes and K+ loss induced by hypotonicity. Chlorpromazine sulfoxide, a relatively ineffective calmodulin antagonist, was considerably less potent as an inhibitor of RVD than chlorpromazine. It is suggested than an elevation in cytoplasmic (Ca++), triggered by cell swelling, increases the plasma membrane permeability to K+, the ensuing increased efflux of K+, associated anions, and osmotically obliged water, leading to cell shrinking (RVD).

  11. WYDOT - Utility Accommodation Regulation Manual | Open Energy...

    Open Energy Info (EERE)

    Regulation ManualPermittingRegulatory GuidanceGuideHandbook Abstract This manual sets forth the processes and procedures for utility accommodation on WYDOT rights-of-way. Author...

  12. Council on Environmental Quality - Regulations for Implementing...

    Open Energy Info (EERE)

    Implementing the Procedural Provisions of the NEPALegal Abstract These regulations set forth the processes and procedures of the National Environmental Policy Act. Published NA...

  13. Alaska Special Area Regulations | Open Energy Information

    Open Energy Info (EERE)

    to library Web Site: Alaska Special Area Regulations Author Alaska Department of Fish & Game Published Publisher Not Provided, 2014 DOI Not Provided Check for DOI...

  14. EPA Regulations Overview Website | Open Energy Information

    Open Energy Info (EERE)

    Overview Website Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: EPA Regulations Overview Website Abstract This website contains information about...

  15. ACHP - Section 106 Regulations Flowchart Explanatory Material...

    Open Energy Info (EERE)

    Explanatory Material Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: ACHP - Section 106 Regulations Flowchart Explanatory Material Abstract This...

  16. Controlling Wind Turbines for Secondary Frequency Regulation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Controlling Wind Turbines for Secondary Frequency Regulation: An Analysis of AGC ... Workshop on Large-Scale Integration of Wind Power Into Power Systems as Well as on ...

  17. Texas Railroad Commission - Pollution Discharge Regulations ...

    Open Energy Info (EERE)

    Not Provided DOI Not Provided Check for DOI availability: http:crossref.org Online Internet link for Texas Railroad Commission - Pollution Discharge Regulations Citation...

  18. Exporting licensing regulations affecting US geothermal firms

    SciTech Connect (OSTI)

    Not Available

    1988-08-01

    This document presents a brief introduction and overview of the Department of Commerce's Export Administration Regulations which might affect potential US geothermal goods exporters. It is intended to make US geothermal firms officials aware of the existence of such regulations and to provide them with references, contacts and phone numbers where they can obtain specific and detailed information and assistance. It must be stressed however, that the ultimate responsibility for complying with the above mentioned regulations lies with the exporter who must consult the complete version of the regulations.

  19. UAC R307 - Air Quality Regulations | Open Energy Information

    Open Energy Info (EERE)

    07 - Air Quality Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: UAC R307 - Air Quality RegulationsLegal...

  20. 5 CCR 1002-62 Colorado Regulations for Effluent Limitations ...

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 5 CCR 1002-62 Colorado Regulations for Effluent LimitationsLegal Abstract Regulations...

  1. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, S.Y.

    1989-08-08

    An improvement in a pump is described including a shrouded inducer, the improvement comprising first and second sealing means which cooperate with a first vortex cell and a series of secondary vortex cells to remove any tangential velocity components from the recirculation flow. 3 figs.

  2. Shrouded inducer pump

    DOE Patents [OSTI]

    Meng, Sen Y.

    1989-01-01

    An improvement in a pump including a shrouded inducer, the improvement comprising first and second sealing means 32,36 which cooperate with a first vortex cell 38 and a series of secondary vortex cells 40 to remove any tangential velocity components from the recirculation flow.

  3. Regulation and policy working group

    SciTech Connect (OSTI)

    1997-03-01

    The potential environmental impact of offshore platform disposal can be illustrated by both the numbers of platforms and the complexity of their abandonment options. Some 7,000 platforms are in place worldwide. In the US, approximately a quarter of the platforms are more than 25 years old and in sight of their end of service. In addition, 22,000 miles of pipeline are located on the Outer Continental Shelf (OCS) in the United States. There are more offshore platforms in the U.S. Gulf of Mexico than in any other single area in the world. It is estimated that between October 1995 and December 2000, approximately 665 of the nearly 3,800 existing structures will be removed. Couple this with the mammoth size, the vagaries of the ocean, and the levels of sometimes conflicting international and federal laws, and the magnitude of the challenge to protect the environment becomes clear. The Offshore International Newsletter (11/06/95) stated, {open_quotes}In three of the last four years, annual Gulf of Mexico platform removals have exceeded installations, a trend that will likely continue.{close_quotes} Between 100 and 150 platforms have been removed from the OCS each year for the past six or seven years. As increasing numbers of wells, pipelines, and platforms are decommissioned and disposed of, it is important that the relevant techniques, policies, and regulations be discussed and evaluated. The goal of this workshop is to facilitate and document this discussion in an open, objective, and inclusive way. Since U.S. practices and policies provide precedents for other countries, international participation is encouraged and anticipated.

  4. Confusion reigns over industrial stormwater regulations

    SciTech Connect (OSTI)

    Bishop, J.

    1993-01-01

    More than five years after Congress mandated controls for industrial and municipal stormwater discharges, many in the regulated community remain unclear about how the rules apply to them. The regulations' progress since the 1987 CWA amendments opened the door for their development often has been shaky and marked by setbacks. According to a federal appeals court decision issued last summer, that struggle is likely to continue. Although the original stormwater rules issued by EPA in November 1990 appeared to focus on heavy manufacturing facilities, as well as municipalities, the appellate court cleared a path that could draw light industrial plants and small construction sites within the regulations' domain. The rules in dispute bring under their umbrella any stormwater discharges associated with industrial activity to surface waters or municipal storm sewer systems. However, the regulations distinguish between facilities engaged in heavy industry and light, or enclosed industrial facilities, based on the probability that stormwater discharges will be contaminated and require regulation.

  5. Polarization induced doped transistor

    DOE Patents [OSTI]

    Xing, Huili; Jena, Debdeep; Nomoto, Kazuki; Song, Bo; Zhu, Mingda; Hu, Zongyang

    2016-06-07

    A nitride-based field effect transistor (FET) comprises a compositionally graded and polarization induced doped p-layer underlying at least one gate contact and a compositionally graded and doped n-channel underlying a source contact. The n-channel is converted from the p-layer to the n-channel by ion implantation, a buffer underlies the doped p-layer and the n-channel, and a drain underlies the buffer.

  6. Laser-Induced Fluorescence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Induced Fluorescence - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced Nuclear

  7. Procyanidin dimer B2-mediated IRAK-M induction negatively regulates TLR4 signaling in macrophages

    SciTech Connect (OSTI)

    Sung, Nak-Yun; Yang, Mi-So; Song, Du-Sub; School of life sciences and Biotechnology, Korea University 5-ka, Anam-Dong, Sungbuk-ku, Seoul 136-701 ; Kim, Jae-Kyung; Park, Jong-Heum; Song, Beom-Seok; Park, Sang-Hyun; Lee, Ju-Woon; Park, Hyun-Jin; Kim, Jae-Hun; Byun, Eui-Baek; Byun, Eui-Hong

    2013-08-16

    Highlights: •Pro B2 elevated the expression of IRAK-M, a negative regulator of TLR signaling. •LPS-induced expression of cell surface molecules was inhibited by Pro B2. •LPS-induced production of pro-inflammatory cytokines was inhibited by Pro B2. •Pro B2 inhibited LPS-induced activation of MAPKs and NF-κB through IRAK-M. •Pro B2 inactivated naïve T cells by inhibiting LPS-induced cytokines via IRAK-M. -- Abstract: Polyphenolic compounds have been found to possess a wide range of physiological activities that may contribute to their beneficial effects against inflammation-related diseases; however, the molecular mechanisms underlying this anti-inflammatory activity are not completely characterized, and many features remain to be elucidated. In this study, we investigated the molecular basis for the down-regulation of toll-like receptor 4 (TLR4) signal transduction by procyanidin dimer B2 (Pro B2) in macrophages. Pro B2 markedly elevated the expression of the interleukin (IL)-1 receptor-associated kinase (IRAK)-M protein, a negative regulator of TLR signaling. Lipopolysaccharide (LPS)-induced expression of cell surface molecules (CD80, CD86, and MHC class I/II) and production of pro-inflammatory cytokines (tumor necrosis factor-α, IL-1β, IL-6, and IL-12p70) were inhibited by Pro B2, and this action was prevented by IRAK-M silencing. In addition, Pro B2-treated macrophages inhibited LPS-induced activation of mitogen-activated protein kinases such as extracellular signal-regulated kinase 1/2, p38, and c-Jun N-terminal kinase and the translocation of nuclear factor κB and p65 through IRAK-M. We also found that Pro B2-treated macrophages inactivated naïve T cells by inhibiting LPS-induced interferon-γ and IL-2 secretion through IRAK-M. These novel findings provide new insights into the understanding of negative regulatory mechanisms of the TLR4 signaling pathway and the immune-pharmacological role of Pro B2 in the immune response against the development

  8. Rainwater harvesting state regulations and technical resources

    SciTech Connect (OSTI)

    Loper, Susan A.

    2015-06-01

    Pacific Northwest National Laboratory (PNNL) conducted in-depth research of state-level rainwater harvesting regulations for the Federal Energy Management Program (FEMP) to help federal agencies strategically identify locations conducive to rainwater harvesting projects. Currently, rainwater harvesting is not regulated by the federal government but rather it is up to individual states to regulate the collection and use of rainwater. There is no centralized information on state-level regulations on rainwater harvesting maintained by a federal agency or outside organization. To fill this information gap, PNNL performed detailed internet searches for each state, which included state agencies, universities, Cooperative Extension Offices, city governments, and related organizations. The state-by-state information on rainwater harvesting regulations was compiled and assembled into an interactive map that is color coded by state regulations. The map provides a visual representation of the general types of rainwater harvesting policies across the country as well as general information on the state programs if applicable. The map allows the user to quickly discern where rainwater harvesting is supported and regulated by the state. This map will be available on the FEMP website by September 2015.

  9. Tight regulation of plant immune responses by combining promoter and suicide exon elements

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gonzalez, Tania L.; Liang, Yan; Nguyen, Bao N.; Staskawicz, Brian J.; Loqué, Dominique; Hammond, Ming C.

    2015-07-02

    Effector-triggered immunity (ETI) is activated when plant disease resistance (R) proteins recognize the presence of pathogen effector proteins delivered into host cells. The ETI response generally encompasses a defensive ‘hypersensitive response’ (HR) that involves programmed cell death at the site of pathogen recognition. While many R protein and effector protein pairs are known to trigger HR, other components of the ETI signaling pathway remain elusive. Effector genes regulated by inducible promoters cause background HR due to leaky protein expression, preventing the generation of relevant transgenic plant lines. By employing the HyP5SM suicide exon, we have developed a strategy to tightlymore » regulate effector proteins such that HR is chemically inducible and non-leaky. This alternative splicing-based gene regulation system was shown to successfully control Bs2/AvrBs2-dependent and RPP1/ATR1Δ51-dependent HR in Nicotiana benthamiana and Nicotiana tabacum, respectively. It was also used to generate viable and healthy transgenic Arabidopsis thaliana plants that inducibly initiate HR. In conclusion, beyond enabling studies on the ETI pathway, our regulatory strategy is generally applicable to reduce or eliminate undesired background expression of transgenes.« less

  10. Tight regulation of plant immune responses by combining promoter and suicide exon elements

    SciTech Connect (OSTI)

    Gonzalez, Tania L.; Liang, Yan; Nguyen, Bao N.; Staskawicz, Brian J.; Loqué, Dominique; Hammond, Ming C.

    2015-07-02

    Effector-triggered immunity (ETI) is activated when plant disease resistance (R) proteins recognize the presence of pathogen effector proteins delivered into host cells. The ETI response generally encompasses a defensive ‘hypersensitive response’ (HR) that involves programmed cell death at the site of pathogen recognition. While many R protein and effector protein pairs are known to trigger HR, other components of the ETI signaling pathway remain elusive. Effector genes regulated by inducible promoters cause background HR due to leaky protein expression, preventing the generation of relevant transgenic plant lines. By employing the HyP5SM suicide exon, we have developed a strategy to tightly regulate effector proteins such that HR is chemically inducible and non-leaky. This alternative splicing-based gene regulation system was shown to successfully control Bs2/AvrBs2-dependent and RPP1/ATR1Δ51-dependent HR in Nicotiana benthamiana and Nicotiana tabacum, respectively. It was also used to generate viable and healthy transgenic Arabidopsis thaliana plants that inducibly initiate HR. In conclusion, beyond enabling studies on the ETI pathway, our regulatory strategy is generally applicable to reduce or eliminate undesired background expression of transgenes.

  11. Chk2 regulates transcription-independent p53-mediated apoptosis in response to DNA damage

    SciTech Connect (OSTI)

    Chen Chen [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan); Shimizu, Shigeomi [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Tsujimoto, Yoshihide [Department of Post-Genomics Diseases, Osaka University Medical School, Suita, Osaka 565-0871 (Japan); Motoyama, Noboru [Department of Geriatric Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522 (Japan)]. E-mail: motoyama@nils.go.jp

    2005-07-29

    The tumor suppressor protein p53 plays a central role in the induction of apoptosis in response to genotoxic stress. The protein kinase Chk2 is an important regulator of p53 function in mammalian cells exposed to ionizing radiation (IR). Cells derived from Chk2-deficient mice are resistant to the induction of apoptosis by IR, and this resistance has been thought to be a result of the defective transcriptional activation of p53 target genes. It was recently shown, however, that p53 itself and histone H1.2 translocate to mitochondria and thereby induces apoptosis in a transcription-independent manner in response to IR. We have now examined whether Chk2 also regulates the transcription-independent induction of apoptosis by p53 and histone H1.2. The reduced ability of IR to induce p53 stabilization in Chk2-deficient thymocytes was associated with a marked impairment of p53 and histone H1 translocation to mitochondria. These results suggest that Chk2 regulates the transcription-independent mechanism of p53-mediated apoptosis by inducing stabilization of p53 in response to IR.

  12. Oxygen-­dependent regulation of bacterial lipid production

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lemmer, Kimberly C.; Dohnalkova, Alice C.; Noguera, Daniel R.; Donohue, Timothy J.

    2015-05-02

    Understanding the mechanisms of lipid accumulation in microorganisms is important for several reasons. In addition to providing insight into assembly of biological membranes, lipid accumulation has important applications in the production of renewable fuels and chemicals. The photosynthetic bacterium Rhodobacter sphaeroides is an attractive organism to study lipid accumulation, as it has the somewhat unique ability to increase membrane production at low O₂ tensions. Under these conditions, R. sphaeroides develops invaginations of the cytoplasmic membrane to increase its membrane surface area for housing of the membrane-bound components of its photosynthetic apparatus. Here we use fatty acid levels as a reportermore » of membrane lipid content. We show that, under low-O₂ and anaerobic conditions, the total fatty acid content per cell increases 3-fold. We also find that the increases in the amount of fatty acid and photosynthetic pigment per cell are correlated as O₂ tensions or light intensity are changed. To ask if lipid and pigment accumulation were genetically separable, we analyzed strains with mutations in known photosynthetic regulatory pathways. While a strain lacking AppA failed to induce photosynthetic pigment-protein complex accumulation, it increased fatty acid content under low O2 conditions. We also found that an intact PrrBA pathway is required for low O2-induced fatty acid accumulation. In conclusion, our findings suggest a previously unknown role of R. sphaeroides transcriptional regulators in increasing fatty acid and phospholipid accumulation in response to decreased O₂ tension.« less

  13. Cell shape regulates global histone acetylation in human mammaryepithelial cells

    SciTech Connect (OSTI)

    Le Beyec, Johanne; Xu, Ren; Lee, Sun-Young; Nelson, Celeste M.; Rizki, Aylin; Alcaraz, Jordi; Bissell, Mina J.

    2007-02-28

    Extracellular matrix (ECM) regulates cell morphology and gene expression in vivo; these relationships are maintained in three-dimensional (3D) cultures of mammary epithelial cells. In the presence of laminin-rich ECM (lrECM), mammary epithelial cells round up and undergo global histone deacetylation, a process critical for their functional differentiation. However, it remains unclear whether lrECM-dependent cell rounding and global histone deacetylation are indeed part of a common physical-biochemical pathway. Using 3D cultures as well as nonadhesive and micropatterned substrata, here we showed that the cell 'rounding' caused by lrECM was sufficient to induce deacetylation of histones H3 and H4 in the absence of biochemical cues. Microarray and confocal analysis demonstrated that this deacetylation in 3D culture is associated with a global increase in chromatin condensation and a reduction in gene expression. Whereas cells cultured on plastic substrata formed prominent stress fibers, cells grown in 3D lrECM or on micropatterns lacked these structures. Disruption of the actin cytoskeleton with cytochalasin D phenocopied the lrECM-induced cell rounding and histone deacetylation. These results reveal a novel link between ECM-controlled cell shape and chromatin structure, and suggest that this link is mediated by changes in the actin cytoskeleton.

  14. Federal Acquisition Regulation Federal Acquisition Circular 2005...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Acquisition Regulation Federal Acquisition Circular 2005-86 Summary of Rules FAC 2005-86 Item Subject FAR Case I Definition of "Multiple-Award Contract" 2015-019 II Sole Source ...

  15. Regulation of geothermal energy development in Colorado

    SciTech Connect (OSTI)

    Coe, B.A.; Forman, N.A.

    1980-01-01

    The regulatory system is presented in a format to help guide geothermal energy development. State, local, and federal agencies, legislation, and regulations are presented. Information sources are listed. (MHR)

  16. Department of Energy Acquisition Regulation (DEAR)

    Office of Energy Efficiency and Renewable Energy (EERE)

    To ensure that you are using the most recent version of the Department of Energy Acquisition Regulation (DEAR), use one of the electronic versions at one of the following websites: DOE Procurement - http://management.energy.gov/DEAR.htm; Electronic Code of Federal Regulations - http://ecfr.gpoaccess.gov/cgi/t/text/text-idx?c=ecfr&tpl=/ecfrbrowse/Tit... or Hill Air Force Base - http://farsite.hill.af.mil/vfdoea.htm.

  17. Searchable Electronic Department of Energy Acquisition Regulation |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Searchable Electronic Department of Energy Acquisition Regulation Searchable Electronic Department of Energy Acquisition Regulation Updated July 2, 2013. The EDEAR is current through the Final Rule published May 3, 2013 at 78 FR 25817 If you have any questions concerning this page, please contact Barbara Binney in the Contract and Financial Assistance Policy Division, Office of Acquisition and Project Management. EDEAR July 2 2013 final.pdf (3.36 MB) More Documents &

  18. International Electricity Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation International Electricity Regulation U.S. trade in electric energy with Canada and Mexico is rising, bringing economic and reliability benefits to the United States and its trading partners. Within the Office of Electricity Delivery & Energy Reliability, the Permitting, Siting and Analysis Division is responsible for authorizing exports of electric energy and issuing Presidential permits for the construction, operation, maintenance and connection of electric transmission

  19. Statutes, Regulations, and Directives for Classification Program |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Statutes, Regulations, and Directives for Classification Program Statutes, Regulations, and Directives for Classification Program Classification Atomic Energy Act of 1954 - Establishes Government-wide policies for classifying, safeguarding, and declassifying Restricted Data information. 10 CFR Part 1045, Nuclear Classification and Declassification - Establishes the Government-wide policies and procedures for implementing sections 141 and 142 of the Atomic Energy Act of

  20. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2008-09-09

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  1. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2003-04-15

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  2. Method for inducing hypothermia

    DOE Patents [OSTI]

    Becker, Lance B.; Hoek, Terry Vanden; Kasza, Kenneth E.

    2005-11-08

    Systems for phase-change particulate slurry cooling equipment and methods to induce hypothermia in a patient through internal and external cooling are provided. Subcutaneous, intravascular, intraperitoneal, gastrointestinal, and lung methods of cooling are carried out using saline ice slurries or other phase-change slurries compatible with human tissue. Perfluorocarbon slurries or other slurry types compatible with human tissue are used for pulmonary cooling. And traditional external cooling methods are improved by utilizing phase-change slurry materials in cooling caps and torso blankets.

  3. REGULATIONS ON PHOTOVOLTAIC MODULE DISPOSAL AND RECYCLING.

    SciTech Connect (OSTI)

    FTHENAKIS,V.

    2001-01-29

    Environmental regulations can have a significant impact on product use, disposal, and recycling. This report summarizes the basic aspects of current federal, state and international regulations which apply to end-of-life photovoltaic (PV) modules and PV manufacturing scrap destined for disposal or recycling. It also discusses proposed regulations for electronics that may set the ground of what is to be expected in this area in the near future. In the US, several states have started programs to support the recycling of electronic equipment, and materials destined for recycling often are excepted from solid waste regulations during the collection, transfer, storage and processing stages. California regulations are described separately because they are different from those of most other states. International agreements on the movement of waste between different countries may pose barriers to cross-border shipments. Currently waste moves freely among country members of the Organization of Economic Cooperation and Development (OECD), and between the US and the four countries with which the US has bilateral agreements. However, it is expected, that the US will adopt the rules of the Basel Convention (an agreement which currently applies to 128 countries but not the US) and that the Convection's waste classification system will influence the current OECD waste-handling system. Some countries adopting the Basel Convention consider end-of-life electronics to be hazardous waste, whereas the OECD countries consider them to be non-hazardous. Also, waste management regulations potentially affecting electronics in Germany and Japan are mentioned in this report.

  4. Troglitazone induces differentiation in Trypanosoma brucei

    SciTech Connect (OSTI)

    Denninger, Viola; Figarella, Katherine; Schoenfeld, Caroline; Brems, Stefanie; Busold, Christian; Lang, Florian; Hoheisel, Joerg; Duszenko, Michael . E-mail: michael.duszenko@uni-tuebingen.de

    2007-05-15

    Trypanosoma brucei, a protozoan parasite causing sleeping sickness, is transmitted by the tsetse fly and undergoes a complex lifecycle including several defined stages within the insect vector and its mammalian host. In the latter, differentiation from the long slender to the short stumpy form is induced by a yet unknown factor of trypanosomal origin. Here we describe that some thiazolidinediones are also able to induce differentiation. In higher eukaryotes, thiazolidinediones are involved in metabolism and differentiation processes mainly by binding to the intracellular receptor peroxisome proliferator activated receptor {gamma}. Our studies focus on the effects of troglitazone on bloodstream form trypanosomes. Differentiation was monitored using mitochondrial markers (membrane potential, succinate dehydrogenase activity, inhibition of oxygen uptake by KCN, amount of cytochrome transcripts), morphological changes (Transmission EM and light microscopy), and transformation experiments (loss of the Variant Surface Glycoprotein coat and increase of dihydroliponamide dehydrogenase activity). To further investigate the mechanisms responsible for these changes, microarray analyses were performed, showing an upregulation of expression site associated gene 8 (ESAG8), a potential differentiation regulator.

  5. Allostery through protein-induced DNA bubbles

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Traverso, Joseph J.; Manoranjan, Valipuram S.; Bishop, A. R.; Rasmussen, Kim Ø.; Voulgarakis, Nikolaos K.

    2015-03-12

    Allostery through DNA is increasingly recognized as an important modulator of DNA functions. Here, we show that the coalescence of protein-induced DNA bubbles can mediate allosteric interactions that drive protein aggregation. We propose that such allostery may regulate DNA's flexibility and the assembly of the transcription machinery. Mitochondrial transcription factor A (TFAM), a dual-function protein involved in mitochondrial DNA (mtDNA) packaging and transcription initiation, is an ideal candidate to test such a hypothesis owing to its ability to locally unwind the double helix. Numerical simulations demonstrate that the coalescence of TFAM-induced bubbles can explain experimentally observed TFAM oligomerization. The resultingmore » melted DNA segment, approximately 10 base pairs long, around the joints of the oligomers act as flexible hinges, which explains the efficiency of TFAM in compacting DNA. Since mitochondrial polymerase (mitoRNAP) is involved in melting the transcription bubble, TFAM may use the same allosteric interaction to both recruit mitoRNAP and initiate transcription.« less

  6. WAC - 173-360 Underground Storage Tank Regulations | Open Energy...

    Open Energy Info (EERE)

    60 Underground Storage Tank Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 173-360 Underground Storage...

  7. Title 48 CFR 1 Federal Acquisition Regulations System | Open...

    Open Energy Info (EERE)

    8 CFR 1 Federal Acquisition Regulations System Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- Federal RegulationFederal Regulation: Title 48 CFR...

  8. WAC 173-400 - General Regulations for Air Pollution Sources ...

    Open Energy Info (EERE)

    400 - General Regulations for Air Pollution Sources Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC 173-400 - General...

  9. WAC - 173-400 General Regulations for Air Pollution Sources ...

    Open Energy Info (EERE)

    400 General Regulations for Air Pollution Sources Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC - 173-400 General...

  10. Form:Federal Oil and Gas Regulation | Open Energy Information

    Open Energy Info (EERE)

    Federal Oil and Gas Regulation Jump to: navigation, search Federal Oil and Gas Regulation This is the "Federal Oil and Gas Regulation" form. To create a page with this form, enter...

  11. Rules and Regulations of the Board of Land Commissioners, Chapter...

    Open Energy Info (EERE)

    Board of Land Commissioners, Chapter 3 - Easements Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Rules and Regulations of...

  12. Rictor regulates FBXW7-dependent c-Myc and cyclin E degradation in colorectal cancer cells

    SciTech Connect (OSTI)

    Guo, Zheng; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, 1838 Guangzhou Dadao Bei, Guangzhou 510515 ; Zhou, Yuning; Evers, B. Mark; Department of Surgery, The University of Kentucky, 800 Rose Street, Lexington, KY 40536 ; Wang, Qingding; Department of Surgery, The University of Kentucky, 800 Rose Street, Lexington, KY 40536

    2012-02-10

    Highlights: Black-Right-Pointing-Pointer Rictor associates with FBXW7 to form an E3 complex. Black-Right-Pointing-Pointer Knockdown of rictor decreases ubiquitination of c-Myc and cylin E. Black-Right-Pointing-Pointer Knockdown of rictor increases protein levels of c-Myc and cylin E. Black-Right-Pointing-Pointer Overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Black-Right-Pointing-Pointer Rictor regulation of c-Myc and cyclin E requires FBXW7. -- Abstract: Rictor (Rapamycin-insensitive companion of mTOR) forms a complex with mTOR and phosphorylates and activates Akt. Activation of Akt induces expression of c-Myc and cyclin E, which are overexpressed in colorectal cancer and play an important role in colorectal cancer cell proliferation. Here, we show that rictor associates with FBXW7 to form an E3 complex participating in the regulation of c-Myc and cyclin E degradation. The Rictor-FBXW7 complex is biochemically distinct from the previously reported mTORC2 and can be immunoprecipitated independently of mTORC2. Moreover, knocking down of rictor in serum-deprived colorectal cancer cells results in the decreased ubiquitination and increased protein levels of c-Myc and cyclin E while overexpression of rictor induces the degradation of c-Myc and cyclin E proteins. Genetic knockout of FBXW7 blunts the effects of rictor, suggesting that rictor regulation of c-Myc and cyclin E requires FBXW7. Our findings identify rictor as an important component of FBXW7 E3 ligase complex participating in the regulation of c-Myc and cyclin E protein ubiquitination and degradation. Importantly, our results suggest that elevated growth factor signaling may contribute to decrease rictor/FBXW7-mediated ubiquitination of c-Myc and cyclin E, thus leading to accumulation of cyclin E and c-Myc in colorectal cancer cells.

  13. Spontaneous Pressure Regulation within Artificial Cells | U.S...

    Office of Science (SC) Website

    Spontaneous Pressure Regulation within Artificial Cells Simple human-made cellular analogues both sense and regulate in response to externally created stress. Print Text Size: A A ...

  14. Contacts for the Assistant General Counsel for Legislation, Regulation...

    Broader source: Energy.gov (indexed) [DOE]

    Office of the Assistant General Counsel for Legislation, Regulation & Energy Efficiency (GC-33) Daniel Cohen, Assistant General Counsel for Legislation, Regulation, & Energy ...

  15. Guide to Federal Regulation of Sales of Imported Electricity...

    Broader source: Energy.gov (indexed) [DOE]

    to Federal Regulation of Sales of Imported Electricity in Canada, Mexico and the United States (155.3 KB) More Documents & Publications North America: Regulation of ...

  16. Class Deviation from the Federal Acquisition Regulation (FAR...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Class Deviation from the Federal Acquisition Regulation (FAR) 13.5, Test Program for Certain Commercial Items Class Deviation from the Federal Acquisition Regulation (FAR) 13.5, ...

  17. EA-1631: Beacon Power Corporation Frequency Regulation Facility...

    Broader source: Energy.gov (indexed) [DOE]

    Frequency Regulation Facility in Stephentown, New York February 27, 2009 EA-1631: Finding of No Significant Impact Loan Guarantee for Beacon Power Corporation Frequency Regulation ...

  18. Arrangement between the Office for Nuclear Regulation of Great...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Arrangement between the Office for Nuclear Regulation of Great Britain and the United ... Arrangement between the Office for Nuclear Regulation of Great Britain and the United ...

  19. Department of Energy Acquisition Regulation (DEAR) Final Rule...

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy Acquisition Regulation (DEAR) Final Rule for changes to Parts 908, ... More Documents & Publications Department of Energy Acquisition Regulation (DEAR) Technical ...

  20. Comments Received on Proposed Rulemaking for regulation implementing...

    Office of Environmental Management (EM)

    for regulation implementing section 216(h): Coordination of Federal Transmission Permitting on Federal Lands Comments Received on Proposed Rulemaking for regulation ...

  1. Regulation Identifier Number Title/Subject/Purpose Rule Type

    Broader source: Energy.gov (indexed) [DOE]

    Regulation Identifier Number TitleSubjectPurpose Rule Type Status 1990-AA40 ... Amend DOE's statutory prescribed regulation, which set forth the procedural rules ...

  2. Fact Sheet: Beacon Power 20 MW Flywheel Frequency Regulation...

    Office of Environmental Management (EM)

    Beacon Power 20 MW Flywheel Frequency Regulation Plant (August 2013) Fact Sheet: Beacon Power 20 MW Flywheel Frequency Regulation Plant (August 2013) Beacon Power will design, ...

  3. Department of Energy Acquisition Regulation (DEAR) Final Rule...

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy Acquisition Regulation (DEAR) Final Rule for Subchapters A, B, and C ... Department of Energy Acquisition Regulation (DEAR) Final Rule for Subchapters A, B, ...

  4. Contacts for the Deputy General Counsel for Litigation, Regulation...

    Office of Environmental Management (EM)

    Litigation, Regulation and Enforcement (GC-30) Contacts for the Deputy General Counsel for Litigation, Regulation and Enforcement (GC-30) Anne Harkavy, Deputy General Counsel for ...

  5. Department of Energy Acquisition Regulation (DEAR) Subpart 903...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Acquisition Regulation (DEAR) Subpart 903.9: Whistleblower Protections for Contractor Employees Department of Energy Acquisition Regulation (DEAR) Subpart ...

  6. NNSA Updates Export Control Regulation | National Nuclear Security...

    National Nuclear Security Administration (NNSA)

    Updates Export Control Regulation February 24, 2015 WASHINGTON, D.C. - The National ... society, NNSA has made Part 810 into a regulation that both meets the requirement to ...

  7. Guide to Federal Regulation of Sales of Imported Electricity...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    GUIDE TO FEDERAL REGULATION OF SALES OF IMPORTED ELECTRICITY IN CANADA, MEXICO, AND THE ... Introduction This Guide to Federal Regulation of Sales of Imported Electricity in Canada, ...

  8. Sandia Energy - Self-Regulated Fabrication of Size-Controlled...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Self-Regulated Fabrication of Size-Controlled Quantum Nanostructures Home Highlights - Energy Research Self-Regulated Fabrication of Size-Controlled Quantum Nanostructures Previous...

  9. Regulation Indentifier Number Title/Subject/Purpose Rule Type

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1991-AB73 Property Management Regulation: Update and eliminate inconsistencies and redundancies in DOE's personal property management regulations at 41 CFR 109. NOPR Drafting 1991...

  10. 5 CCR 1002-61 Colorado Discharge Permit System Regulations |...

    Open Energy Info (EERE)

    Discharge Permit System RegulationsLegal Abstract Regulations implementing the Colorado Water Quality Control Act applying to all operations discharging to waters of the state...

  11. Notification of Regulated Waste Activity | Open Energy Information

    Open Energy Info (EERE)

    Notification of Regulated Waste Activity Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- OtherOther: Notification of Regulated Waste...

  12. California Regulations on Renewble Hydrogen and Low Carbon Technologie...

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    Regulations on Renewble Hydrogen and Low Carbon Technologies California Regulations on ... Transportation and Stationary Power Integration Workshop: A California Perspective Vision ...

  13. June 24 Webinar Will Discuss How Environmental Laws and Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Will Discuss How Environmental Laws and Regulations Affect Tribal Energy Projects June 24 Webinar Will Discuss How Environmental Laws and Regulations Affect Tribal Energy...

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    Open Energy Info (EERE)

    Laws, Policies and Regulations Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Renewable Energy Terms of Reference: Laws, Policies and Regulations AgencyCompany...

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    Participation of the EM SSAB, Stakeholders, and Regulators in EM Budget Requests Memorandum: Participation of the EM SSAB, Stakeholders, and Regulators in EM Budget Requests From: ...

  16. Self-Regulation Mechanism for Charged Point Defects in Hybrid...

    Office of Scientific and Technical Information (OSTI)

    Accepted Manuscript: Self-Regulation Mechanism for Charged Point Defects in Hybrid Halide Perovskites Title: Self-Regulation Mechanism for Charged Point Defects in Hybrid Halide ...

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    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Demonstration of 2010 Emissions Regulations over Transient Operation Technical Demonstration of 2010 Emissions Regulations over Transient Operation Presentation given at DEER 2006, ...

  18. Federal Register Notices for DOE NEPA Guidelines and Regulations...

    Office of Environmental Management (EM)

    Notices for DOE NEPA Guidelines and Regulations Federal Register Notices for DOE NEPA Guidelines and Regulations Historical compilation of Federal Register notices for DOE NEPA...

  19. SERN Policy and Regulation Database | Open Energy Information

    Open Energy Info (EERE)

    SERN Policy and Regulation Database Jump to: navigation, search Tool Summary Name: SERN Policy and Regulation Database AgencyCompany Organization: Renewable Energy and Energy...

  20. New Tax Regulations Help Further Low Income Solar | Department...

    Energy Savers [EERE]

    The final regulations state that utility costs paid by a tenant are treated the same as submitting payment directly to the utility company. The temporary regulations extend the ...

  1. EO 13211: Regulations That Significantly Affect Energy Supply...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EO 13211: Regulations That Significantly Affect Energy Supply, Distribution, or Use EO 13211: Regulations That Significantly Affect Energy Supply, Distribution, or Use I am...

  2. Natural Gas Regulation - Other Gas-Related Information Sources...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Natural Gas Regulation - Other Gas-Related Information Sources Natural Gas Regulation - Other Gas-Related Information Sources The single largest source of energy information...

  3. New Mexico Public Regulation Commission | Open Energy Information

    Open Energy Info (EERE)

    Public Regulation Commission Name: New Mexico Public Regulation Commission Address: 33 Plaza La Prensa Place: Santa Fe, New Mexico Zip: 87507 Website: www.nmprc.state.nm.us...

  4. Category:Federal Oil and Gas Regulations | Open Energy Information

    Open Energy Info (EERE)

    Regulations Jump to: navigation, search Add a new Federal Oil and Gas Regulation This category currently contains no pages or media. Retrieved from "http:en.openei.orgw...

  5. Idaho IS 61 Public Utility Regulation | Open Energy Information

    Open Energy Info (EERE)

    Regulation (2013). Retrieved from "http:en.openei.orgwindex.php?titleIdahoIS61PublicUtilityRegulation&oldid722586" Feedback Contact needs updating Image needs...

  6. Building Codes and Regulations for Solar Water Heating Systems...

    Office of Environmental Management (EM)

    Building Codes and Regulations for Solar Water Heating Systems Building Codes and Regulations for Solar Water Heating Systems June 24, 2012 - 1:50pm Addthis Photo Credit:...

  7. Non-fibrillar amyloid-{beta} peptide reduces NMDA-induced neurotoxicity, but not AMPA-induced neurotoxicity

    SciTech Connect (OSTI)

    Niidome, Tetsuhiro; Goto, Yasuaki; Kato, Masaru; Wang, Pi-Lin; Goh, Saori; Tanaka, Naoki; Akaike, Akinori; Kihara, Takeshi; Sugimoto, Hachiro

    2009-09-04

    Amyloid-{beta} peptide (A{beta}) is thought to be linked to the pathogenesis of Alzheimer's disease. Recent studies suggest that A{beta} has important physiological roles in addition to its pathological roles. We recently demonstrated that A{beta}42 protects hippocampal neurons from glutamate-induced neurotoxicity, but the relationship between A{beta}42 assemblies and their neuroprotective effects remains largely unknown. In this study, we prepared non-fibrillar and fibrillar A{beta}42 based on the results of the thioflavin T assay, Western blot analysis, and atomic force microscopy, and examined the effects of non-fibrillar and fibrillar A{beta}42 on glutamate-induced neurotoxicity. Non-fibrillar A{beta}42, but not fibrillar A{beta}42, protected hippocampal neurons from glutamate-induced neurotoxicity. Furthermore, non-fibrillar A{beta}42 decreased both neurotoxicity and increases in the intracellular Ca{sup 2+} concentration induced by N-methyl-D-aspartate (NMDA), but not by {alpha}-amino-3-hydrozy-5-methyl-4-isoxazole propionic acid (AMPA). Our results suggest that non-fibrillar A{beta}42 protects hippocampal neurons from glutamate-induced neurotoxicity through regulation of the NMDA receptor.

  8. Honduras geothermal development: Regulations and opportunities

    SciTech Connect (OSTI)

    Goff, S.J.; Winchester, W.W.

    1994-09-01

    The US Department of Energy (DOE) through the Assistant Secretary for Policy, Planning, and Evaluation funded a project to review and evaluate existing power sector laws and regulations in Honduras. Also included in the scope of the project was a review of regulations pertaining to the privatization of state-run companies. We paid particular attention to regulations which might influence opportunities to develop and commercialize Honduras` geothermal resources. We believe that Honduras is well on the road to attracting foreign investment and has planned or has already in place much of the infrastructure and legal guarantees which encourage the influx of private funds from abroad. In addition, in light of current power rationing and Honduras` new and increasing awareness of the negative effects of power sector development on the environment, geothermal energy development is even more attractive. Combined, these factors create a variety of opportunities. The potential for private sector development of geothermal positive.

  9. Historical impacts of environmental regulation of silver

    SciTech Connect (OSTI)

    Purcell, T.W.; Peters, J.J.

    1999-01-01

    Exposure to silver, and hence its possible environmental effects, has been regulated since the 1960s. Rules were first developed in the United States to protect against potential negative effects to humans due to ingestion of drinking water containing silver. As regulations for other environmental media were developed, silver was generally included because of its designation as a drinking water chemical of concern. Ambient water quality criteria were developed based on the toxic action of ionic silver. In the early 1990s the US Environmental Protection Agency`s Office of Drinking Water downgraded silver from a primary to a secondary maximum contaminant level because the effects of exposure to silver were judged to be cosmetic. Regulations pertaining to solid waste, surface waters, and occupational exposure have not been changed, but may be changed in the near future as the fate and toxic mechanisms of silver become better understood.

  10. Environmental regulations handbook for enhanced oil recovery

    SciTech Connect (OSTI)

    Madden, M.P. ); Blatchford, R.P.; Spears, R.B. )

    1991-12-01

    This handbook is intended to assist owners and operators of enhanced oil recovery (EOR) operations in acquiring some introductory knowledge of the various state agencies, the US Environmental Protection Agency, and the many environmental laws, rules and regulations which can have jurisdiction over their permitting and compliance activities. It is a compendium of summarizations of environmental rules. It is not intended to give readers specific working details of what is required from them, nor can it be used in that manner. Readers of this handbook are encouraged to contact environmental control offices nearest to locations of interest for current regulations affecting them.

  11. Oxygen-­dependent regulation of bacterial lipid production

    SciTech Connect (OSTI)

    Lemmer, Kimberly C.; Dohnalkova, Alice C.; Noguera, Daniel R.; Donohue, Timothy J.

    2015-05-02

    Understanding the mechanisms of lipid accumulation in microorganisms is important for several reasons. In addition to providing insight into assembly of biological membranes, lipid accumulation has important applications in the production of renewable fuels and chemicals. The photosynthetic bacterium Rhodobacter sphaeroides is an attractive organism to study lipid accumulation, as it has the somewhat unique ability to increase membrane production at low O₂ tensions. Under these conditions, R. sphaeroides develops invaginations of the cytoplasmic membrane to increase its membrane surface area for housing of the membrane-bound components of its photosynthetic apparatus. Here we use fatty acid levels as a reporter of membrane lipid content. We show that, under low-O₂ and anaerobic conditions, the total fatty acid content per cell increases 3-fold. We also find that the increases in the amount of fatty acid and photosynthetic pigment per cell are correlated as O₂ tensions or light intensity are changed. To ask if lipid and pigment accumulation were genetically separable, we analyzed strains with mutations in known photosynthetic regulatory pathways. While a strain lacking AppA failed to induce photosynthetic pigment-protein complex accumulation, it increased fatty acid content under low O2 conditions. We also found that an intact PrrBA pathway is required for low O2-induced fatty acid accumulation. In conclusion, our findings suggest a previously unknown role of R. sphaeroides transcriptional regulators in increasing fatty acid and phospholipid accumulation in response to decreased O₂ tension.

  12. Voltage regulation in linear induction accelerators

    DOE Patents [OSTI]

    Parsons, William M.

    1992-01-01

    Improvement in voltage regulation in a Linear Induction Accelerator wherein a varistor, such as a metal oxide varistor, is placed in parallel with the beam accelerating cavity and the magnetic core. The non-linear properties of the varistor result in a more stable voltage across the beam accelerating cavity than with a conventional compensating resistance.

  13. Voltage regulation in linear induction accelerators

    DOE Patents [OSTI]

    Parsons, W.M.

    1992-12-29

    Improvement in voltage regulation in a linear induction accelerator wherein a varistor, such as a metal oxide varistor, is placed in parallel with the beam accelerating cavity and the magnetic core is disclosed. The non-linear properties of the varistor result in a more stable voltage across the beam accelerating cavity than with a conventional compensating resistance. 4 figs.

  14. Method for regulation of plant lignin composition

    DOE Patents [OSTI]

    Chapple, Clint

    1999-01-01

    A method is disclosed for the regulation of lignin composition in plant tissue. Plants are transformed with a gene encoding an active F5H gene. The expression of the F5H gene results in increased levels of syringyl monomer providing a lignin composition more easily degraded with chemicals and enzymes.

  15. Coping with uncertainties of mercury regulation

    SciTech Connect (OSTI)

    Reich, K.

    2006-09-15

    The thermometer is rising as coal-fired plants cope with the uncertainties of mercury regulation. The paper deals with a diagnosis and a suggested cure. It describes the state of mercury emission rules in the different US states, many of which had laws or rules in place before the Clean Air Mercury Rule (CAMR) was promulgated.

  16. State-federal interactions in nuclear regulation

    SciTech Connect (OSTI)

    Pasternak, A.D.; Budnitz, R.J.

    1987-12-01

    The Atomic Energy Act of 1954 established, and later Congressional amendments have confirmed, that except in areas which have been explicitly granted to the states, the federal government possesses preemptive authority to regulate radiation hazards associated with the development and use of atomic energy. Since the passage of the original Act, numerous decisions by the courts have reaffirmed the legitimacy of federal preemption, and have defined and redefined its scope. In this study, the aim is to explore the underlying issues involved in federal preemption of radiation-hazard regulation, and to recommend actions that the Department of Energy and other agencies and groups should consider undertaking in the near term to protect the preemption principle. Appropriate roles of the states are discussed, as well as recent state-level activities and their rationale, and several current arenas in which state-federal conflicts about regulation of hazards are being played out. The emphasis here is on four particular arenas that are now important arenas of conflict, but the issues discussed are far broader in scope. These four arenas are: state-level moratorium activity; emergency planning for reactors; conflicts arising from state financial regulation; and inroads in federal preemption through litigation under state law.

  17. Src regulates the activity of SIRT2

    SciTech Connect (OSTI)

    Choi, You Hee; Kim, Hangun; Lee, Sung Ho; Jin, Yun-Hye; Lee, Kwang Youl

    2014-07-25

    Highlights: Src decreases the protein levels of Sirt2. Src inhibitor and knockdown of Src increase the protein levels of Sirt2. Src interacts with and phosphorylates Sirt2. Src regulate the activity of Sirt2. - Abstract: SIRT2 is a mammalian member of the Sirtuin family of NAD{sup +}-dependent protein deacetylases. The tyrosine kinase Src is involved in a variety of cellular signaling pathways, leading to the induction of DNA synthesis, cell proliferation, and cytoskeletal reorganization. The function of SIRT2 is modulated by post-translational modifications; however, the precise molecular signaling mechanism of SIRT2 through interactions with c-Src has not yet been established. In this study, we investigated the potential regulation of SIRT2 function by c-Src. We found that the protein levels of SIRT2 were decreased by c-Src, and subsequently rescued by the addition of a Src specific inhibitor, SU6656, or by siRNA-mediated knockdown of c-Src. The c-Src interacts with and phosphorylates SIRT2 at Tyr104. c-Src also showed the ability to regulate the deacetylation activity of SIRT2. Investigation on the phosphorylation of SIRT2 suggested that this was the method of c-Src-mediated SIRT2 regulation.

  18. Federal Regulations | U.S. DOE Office of Science (SC)

    Office of Science (SC) Website

    Federal Regulations Human Subjects Protection Program (HSPP) HSPP Home About Institutional Review Boards (IRBs) Education and Resources Regulations and Requirements Ethical Principles Federal Regulations DOE Special Requirements Health Insurance Portability and Accountability Act (HIPAA) Glossary Abbreviations Contact BER Home Contact Information Human Subjects Protection Program U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 Regulations and

  19. 49 CFR Parts 171-177: Hazardous Materials Regulations (DOT)

    Broader source: Energy.gov [DOE]

    The U.S. Department of Transportation Pipeline and Hazardous Materials Safety Administration regulates the transport of hazardous materials through Title 49 of the Code of Federal Regulations (49 CFR), Subchapter C, "Hazardous Materials Regulations." Parts 171-177 provide general information on hazardous materials and regulation for their packaging and their shipment by rail, air, vessel, and public highway.

  20. Oxidative stress-induced autophagy: Role in pulmonary toxicity

    SciTech Connect (OSTI)

    Malaviya, Rama; Laskin, Jeffrey D.; Laskin, Debra L.

    2014-03-01

    Autophagy is an evolutionarily conserved catabolic process important in regulating the turnover of essential proteins and in elimination of damaged organelles and protein aggregates. Autophagy is observed in the lung in response to oxidative stress generated as a consequence of exposure to environmental toxicants. Whether autophagy plays role in promoting cell survival or cytotoxicity is unclear. In this article recent findings on oxidative stress-induced autophagy in the lung are reviewed; potential mechanisms initiating autophagy are also discussed. A better understanding of autophagy and its role in pulmonary toxicity may lead to the development of new strategies to treat lung injury associated with oxidative stress. - Highlights: • Exposure to pulmonary toxicants is associated with oxidative stress. • Oxidative stress is known to induce autophagy. • Autophagy is upregulated in the lung following exposure to pulmonary toxicants. • Autophagy may be protective or pathogenic.

  1. Notice of 2009 Guidance on Energy Efficiency Enforcement Regulations |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy 2009 Guidance on Energy Efficiency Enforcement Regulations Notice of 2009 Guidance on Energy Efficiency Enforcement Regulations Notice - DOE Guidance on Energy Efficiency Enforcement Regulations, Federal Register Vol. 74, No. 197 October 14, 2009. This notice sets forth the Department of Energy's (DOE's) interpretation of its energy efficiency enforcement regulations. These regulations provide for manufacturer submission of compliance statements and certification reports

  2. Policy and Regulation Technical Assistance | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Policy and Regulation Technical Assistance Policy and Regulation Technical Assistance Policy and regulation technical assistance includes information and guidance for Indian tribes and Alaska Native villages on energy governance issues. Below is a list of the various options for this type of technical assistance. To apply for policy, regulation, and codes technical assistance, complete the online technical assistance request form. Policy and Regulation Technical Assistance Options Tribal energy

  3. Collegiate Wind Competition 2014 Rules and Regulations | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy 2014 Rules and Regulations Collegiate Wind Competition 2014 Rules and Regulations This document outlines the rules and regulations for the inaugural Collegiate Wind Competition in 2014. U.S. Department of Energy Collegiate Wind Competition 2014 Rules and Regulations (1.62 MB) More Documents & Publications Collegiate Wind Competition 2016 Rules and Regulations Team roster: Andrew Dallas, Aerospace Engineering; Mario Mondal, Aerospace Engineering; Atif Salahudeen, Aerospace

  4. Apigenin suppresses migration and invasion of transformed cells through down-regulation of C-X-C chemokine receptor 4 expression

    SciTech Connect (OSTI)

    Wang, Lei; Kuang, Lisha; Hitron, John Andrew; Son, Young-Ok; Wang, Xin; Budhraja, Amit; Lee, Jeong-Chae; Pratheeshkumar, Poyil; Chen, Gang; Zhang, Zhuo; Luo, Jia; Shi, Xianglin

    2013-10-01

    Environmental exposure to arsenic is known to cause various cancers. There are some potential relationships between cell malignant transformation and C-X-C chemokine receptor type 4 (CXCR4) expressions. Metastasis, one of the major characteristics of malignantly transformed cells, contributes to the high mortality of cells. CXCR4 and its natural chemokine ligand C-X-C motif ligand 12 (CXCL12) play a critical role in metastasis. Therefore, identification of nutritional factors which are able to inhibit CXCR4 is important for protection from environmental arsenic-induced carcinogenesis and for abolishing metastasis of malignantly transformed cells. The present study demonstrates that apigenin (4′,5,7-trihydroxyflavone), a natural dietary flavonoid, suppressed CXCR4 expression in arsenic-transformed Beas-2B cells (B-AsT) and several other types of transformed/cancer cells in a dose- and time-dependent manner. Neither proteasome nor lysosome inhibitor had any effect in reducing the apigenin-induced down-regulation of CXCR4, indicating that apigenin-induced down-regulation of CXCR4 is not due to proteolytic degradation. The down-regulation of CXCR4 is mainly due to the inhibition of nuclear factor κB (NF-κB) transcriptional activity. Apigenin also abolished migration and invasion of transformed cells induced by CXCL12. In a xenograft mouse model, apigenin down-regulated CXCR4 expression and suppressed tumor growth. Taken together, our results show that apigenin is a novel inhibitor of CXCR4 expression. This dietary flavonoid has the potential to suppress migration and invasion of transformed cells and prevent environmental arsenic-induced carcinogenesis. - Highlights: • Apigenin has a potential in preventing environmental arsenic induced carcinogenesis. • Apigenin suppresses CXCR4 in malignant transformed cells in vitro and in vivo. • The down-regulation of CXCR4 is mainly due to inhibition of NF-κB activity.

  5. Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation

    SciTech Connect (OSTI)

    Reed, J.C.; Tsujimoto, Y.; Alpers, J.D.; Croce, C.M.; Nowell, P.C.

    1987-06-05

    The bcl-2 and c-myc proto-oncogenes are brought into juxtaposition with the immuno-globulin heavy chain locus in particular B-cell lymphomas, resulting in high levels of constitutive accumulation of their messenger RNAs. Precisely how the products of the bcl-2 and c-myc genes contribute to tumorigenesis is unknown, but observations that c-myc expression is rapidly induced in nonneoplastic lymphocytes upon stimulation of proliferation raise the possibility that this proto-oncogene is involved in the control of normal cellular growth. In addition to c-myc, the bcl-2 proto-oncogene also was expressed in normal human B and T lymphocytes after stimulation with appropriate mitogens. Comparison of the regulation of the expression of these proto-oncogenes demonstrated marked differences and provided evidence that, in contrast to c-myc, levels of bcl-2 messenger RNA are regulated primarily though transcriptional mechanisms. 10 references, 3 figures.

  6. Mammalian aPKC/Par polarity complex mediated regulation of epithelial division orientation and cell fate

    SciTech Connect (OSTI)

    Vorhagen, Susanne; Niessen, Carien M.

    2014-11-01

    Oriented cell division is a key regulator of tissue architecture and crucial for morphogenesis and homeostasis. Balanced regulation of proliferation and differentiation is an essential property of tissues not only to drive morphogenesis but also to maintain and restore homeostasis. In many tissues orientation of cell division is coupled to the regulation of differentiation producing daughters with similar (symmetric cell division, SCD) or differential fate (asymmetric cell division, ACD). This allows the organism to generate cell lineage diversity from a small pool of stem and progenitor cells. Division orientation and/or the ratio of ACD/SCD need to be tightly controlled. Loss of orientation or an altered ratio can promote overgrowth, alter tissue architecture and induce aberrant differentiation, and have been linked to morphogenetic diseases, cancer and aging. A key requirement for oriented division is the presence of a polarity axis, which can be established through cell intrinsic and/or extrinsic signals. Polarity proteins translate such internal and external cues to drive polarization. In this review we will focus on the role of the polarity complex aPKC/Par3/Par6 in the regulation of division orientation and cell fate in different mammalian epithelia. We will compare the conserved function of this complex in mitotic spindle orientation and distribution of cell fate determinants and highlight common and differential mechanisms in which this complex is used by tissues to adapt division orientation and cell fate to the specific properties of the epithelium.

  7. miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

    SciTech Connect (OSTI)

    Mizuno, Yosuke; Yagi, Ken; Tokuzawa, Yoshimi; Kanesaki-Yatsuka, Yukiko; Suda, Tatsuo; Katagiri, Takenobu; Fukuda, Toru; Maruyama, Masayoshi; Okuda, Akihiko; Amemiya, Tomoyuki; Kondoh, Yasumitsu; Tashiro, Hideo; Okazaki, Yasushi

    2008-04-04

    Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

  8. ADR Provisions in Federal Acquisition Regulation (FAR)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ADR Provisions in Federal Acquisition Regulation (FAR) d. Alternative means of dispute resolution. Notwithstanding any other provision of this chapter, a contractor and a contracting officer may use any alternative means of dispute resolution under subchapter IV of chapter 5 of title 5, or other mutually agreeable procedures, for resolving claims. In a case in which such alternative means of dispute resolution or other mutually agreeable procedures are used, the contractor shall certify that the

  9. Regulation of flexible arms under gravity

    SciTech Connect (OSTI)

    De Luca, A.; Siciliano, B.

    1993-08-01

    A simple controller is presented for the regulation problem of robot arms with flexible links under gravity. It consists of a joint PD feedback plus a constant feedforward. Global asymptotic stability of the reference equilibrium state is shown under a structural assumption about link elasticity and a mild condition on the proportional gain. The result holds also in the absence of internal damping of the flexible arm. A numerical case study is presented.

  10. Competition law and British natural gas regulation

    SciTech Connect (OSTI)

    Black, A.J.

    1992-01-01

    British Gas (BG) is a seriously successful monopolist which, since its 1986 privatisation, is acing increased regulation by the Office of Gas Supply (OFGAS). OFGAS is the first public body specifically created to regulate a European gas industry. It employs a rate-capping formula instead of the more labour intensive rate-of-retum method favoured in North America. Despite initial criticisms, OFGAS has surprised industry observers with efficacious results. This article succinctly discusses the process of natural gas industry privatisation in the United Kingdom (U.K.) and the development of a British type of [open quotes]open access.[close quotes] Contemporary British gas regulation is a distinct paradigm involving the privatisation of a vertically integrated pipeline system coupled with an altemative regulatory method. These regulatory results include lower prices for core customers and the promotion of third party direct sales within the U.K. Since Britain leads the European Community (E.C.) in common carriage provisions, the regulatory r6gime here provides a benchmark for the other Member States.

  11. Microscopic Description of Induced Nuclear Fission (Conference...

    Office of Scientific and Technical Information (OSTI)

    Microscopic Description of Induced Nuclear Fission Citation Details In-Document Search Title: Microscopic Description of Induced Nuclear Fission You are accessing a document ...

  12. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Disorder-Induced Microscopic Magnetic Memory Print Wednesday, 26 October 2005 00:00 The magnetic-recording industry deliberately...

  13. Solitosynthesis induced phase transitions (Journal Article) ...

    Office of Scientific and Technical Information (OSTI)

    Solitosynthesis induced phase transitions Citation Details In-Document Search Title: Solitosynthesis induced phase transitions Authors: Pearce, Lauren Publication Date: 2012-06-19 ...

  14. Solitosynthesis induced phase transitions (Journal Article) ...

    Office of Scientific and Technical Information (OSTI)

    Solitosynthesis induced phase transitions Prev Next Title: Solitosynthesis induced phase transitions Authors: Pearce, Lauren Publication Date: 2012-06-19 OSTI Identifier: ...

  15. Condensation induced water hammer safety (Technical Report) ...

    Office of Scientific and Technical Information (OSTI)

    Condensation induced water hammer safety Citation Details In-Document Search Title: Condensation induced water hammer safety You are accessing a document from the Department of ...

  16. Laser Induced Spectroscopy - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laser Induced Spectroscopy Idaho National Laboratory Contact INL About This Technology Technology Marketing Summary INL's Laser Induced Spectroscopy technology detects and measures ...

  17. Mechanisms of the hepatoprotective effects of tamoxifen against drug-induced and chemical-induced acute liver injuries

    SciTech Connect (OSTI)

    Yoshikawa, Yukitaka; Miyashita, Taishi; Higuchi, Satonori; Tsuneyama, Koichi; Endo, Shinya; Tsukui, Tohru; Toyoda, Yasuyuki; Fukami, Tatsuki; Nakajima, Miki; Yokoi, Tsuyoshi

    2012-10-01

    Although estrogen receptor (ER)? agonists, such as estradiol and ethinylestradiol (EE2), cause cholestasis in mice, they also reduce the degree of liver injury caused by hepatotoxicants as well as ischemiareperfusion. The functional mechanisms of ER? have yet to be elucidated in drug-induced or chemical-induced liver injury. The present study investigated the effects of an ER? agonist, selective ER modulators (SERMs) and an ER antagonist on drug-induced and chemical-induced liver injuries caused by acetaminophen, bromobenzene, diclofenac, and thioacetamide (TA). We observed hepatoprotective effects of EE2, tamoxifen (TAM) and raloxifene pretreatment in female mice that were exposed to a variety of hepatotoxic compounds. In contrast, the ER antagonist did not show any hepatoprotective effects. DNA microarray analyses suggested that monocyte to macrophage differentiation-associated 2 (Mmd2) protein, which has an unknown function, is commonly increased by TAM and RAL pretreatment, but not by pretreatment with the ER antagonist. In ER?-knockout mice, the hepatoprotective effects of TAM and the increased expression of Mmd2 mRNA were not observed in TA-induced liver injury. To investigate the function of Mmd2, the expression level of Mmd2 mRNA was significantly knocked down to approximately 30% in mice by injection of siRNA for Mmd2 (siMmd2). Mmd2 knockdown resulted in a reduction of the protective effects of TAM on TA-induced liver injury in mice. This is the first report of the involvement of ER? in drug-induced or chemical-induced liver injury. Upregulation of Mmd2 protein in the liver was suggested as the mechanism of the hepatoprotective effects of EE2 and SERMs. -- Highlights: ? Liver injury induced by drugs or chemicals was investigated in mice. ? Liver injury was suppressed by pretreatment with tamoxifen in female mice. ? Mmd2, whose function was unknown, could be a candidate gene for liver protection. ? Tamoxifen up-regulated Mmd2 mRNA expression via ER?.

  18. Platelet-derived growth factor regulates vascular smooth muscle phenotype via mammalian target of rapamycin complex 1

    SciTech Connect (OSTI)

    Ha, Jung Min; Yun, Sung Ji; Kim, Young Whan; Jin, Seo Yeon; Lee, Hye Sun; Song, Sang Heon; Shin, Hwa Kyoung; Bae, Sun Sik

    2015-08-14

    Mammalian target of rapamycin complex (mTORC) regulates various cellular processes including proliferation, growth, migration and differentiation. In this study, we showed that mTORC1 regulates platelet-derived growth factor (PDGF)-induced phenotypic conversion of vascular smooth muscle cells (VSMCs). Stimulation of contractile VSMCs with PDGF significantly reduced the expression of contractile marker proteins in a time- and dose-dependent manner. In addition, angiotensin II (AngII)-induced contraction of VSMCs was completely blocked by the stimulation of VSMCs with PDGF. PDGF-dependent suppression of VSMC marker gene expression was significantly blocked by inhibition of phosphatidylinositol 3-kinase (PI3K), extracellular signal-regulated kinase (ERK), and mTOR whereas inhibition of p38 MAPK had no effect. In particular, inhibition of mTORC1 by rapamycin or by silencing of Raptor significantly blocked the PDGF-dependent phenotypic change of VSMCs whereas silencing of Rictor had no effect. In addition, loss of AngII-dependent contraction by PDGF was significantly retained by silencing of Raptor. Inhibition of mTORC1 by rapamycin or by silencing of Raptor significantly blocked PDGF-induced proliferation of VSMCs. Taken together, we suggest that mTORC1 plays an essential role in PDGF-dependent phenotypic changes of VSMCs. - Graphical abstract: Regulation of VSMC phenotype by PDGF-dependent activation of mTORC1. - Highlights: • The expression of contractile marker proteins was reduced by PDGF stimulation. • PDGF-dependent phenotypic conversion of VSMCs was blocked by inhibition of mTOR. • PDGF-induced proliferation of VSMCs was attenuated by inhibition of mTORC1. • mTORC1 plays a critical role in PDGF-dependent phenotypic conversion of VSMCs.

  19. Hedgehog pathway regulators influence cervical cancer cell proliferation, survival and migration

    SciTech Connect (OSTI)

    Samarzija, Ivana; Beard, Peter

    2012-08-17

    Highlights: Black-Right-Pointing-Pointer Unknown cellular mutations complement papillomavirus-induced carcinogenesis. Black-Right-Pointing-Pointer Hedgehog pathway components are expressed by cervical cancer cells. Black-Right-Pointing-Pointer Hedgehog pathway activators and inhibitors regulate cervical cancer cell biology. Black-Right-Pointing-Pointer Cell immortalization by papillomavirus and activation of Hedgehog are independent. -- Abstract: Human papillomavirus (HPV) infection is considered to be a primary hit that causes cervical cancer. However, infection with this agent, although needed, is not sufficient for a cancer to develop. Additional cellular changes are required to complement the action of HPV, but the precise nature of these changes is not clear. Here, we studied the function of the Hedgehog (Hh) signaling pathway in cervical cancer. The Hh pathway can have a role in a number of cancers, including those of liver, lung and digestive tract. We found that components of the Hh pathway are expressed in several cervical cancer cell lines, indicating that there could exists an autocrine Hh signaling loop in these cells. Inhibition of Hh signaling reduces proliferation and survival of the cervical cancer cells and induces their apoptosis as seen by the up-regulation of the pro-apoptotic protein cleaved caspase 3. Our results indicate that Hh signaling is not induced directly by HPV-encoded proteins but rather that Hh-activating mutations are selected in cells initially immortalized by HPV. Sonic Hedgehog (Shh) ligand induces proliferation and promotes migration of the cervical cancer cells studied. Together, these results indicate pro-survival and protective roles of an activated Hh signaling pathway in cervical cancer-derived cells, and suggest that inhibition of this pathway may be a therapeutic option in fighting cervical cancer.

  20. Fibroblast growth factor-2 up-regulates the expression of nestin through the RasRafERKSp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: Nestin expression in C6 glioma cells is induced by FGF-2. Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. The mRNA of FGFR1 and 3 are detected in C6 glioma cells. RasRafERKSp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFRMAPKERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  1. Evolving Role of the Power Sector Regulator: A Clean Energy Regulators Initiative Report

    SciTech Connect (OSTI)

    Zinaman, O.; Miller, M.; Bazilian, M.

    2014-04-01

    This paper seeks to briefly characterize the evolving role of power sector regulation. Given current global dynamics, regulation of the power sector is undergoing dramatic changes. This transformation is being driven by various factors including technological advances and cost reductions in renewable energy, energy efficiency, and demand management; increasing air pollution and climate change concerns; and persistent pressure for ensuring sustainable economic development and increased access to energy services by the poor. These issues add to the already complex task of power sector regulation, of which the fundamental remit remains to objectively and transparently ensure least-cost service delivery at high quality. While no single regulatory task is trivial to undertake, it is the prioritization and harmonization of a multitude of objectives that exemplifies the essential challenge of power sector regulation. Evolving regulatory roles can be understood through the concept of existing objectives and an additional layer of emerging objectives. Following this categorization, we describe seven existing objectives of power sector regulators and nine emerging objectives, highlighting key challenges and outlining interdependencies. This essay serves as a preliminary installment in the Clean Energy Regulatory Initiative (CERI) series, and aims to lay the groundwork for subsequent reports and case studies that will explore these topics in more depth.

  2. Wnt/{beta}-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression

    SciTech Connect (OSTI)

    Zhang, Long; Shi, Songting; Zhang, Juan; Zhou, Fangfang; Dijke, Peter ten

    2012-03-02

    Highlights: Black-Right-Pointing-Pointer Expression of Id3 but not Id1 is induced by Wnt3a stimulation in C2C12 cells. Black-Right-Pointing-Pointer Wnt3a induces Id3 expression via canonical Wnt/{beta}-catenin pathway. Black-Right-Pointing-Pointer Wnt3a-induced Id3 expression does not depend on BMP signaling activation. Black-Right-Pointing-Pointer Induction of Id3 expression is critical determinant in Wnt3a-induced cell proliferation and differentiation. -- Abstract: Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a {beta}-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/{beta}-catenin induced gene in myoblast cell fate determination.

  3. EGA urges regulators to rethink utility structure

    SciTech Connect (OSTI)

    O'Driscoll, M.

    1994-03-04

    State and federal regulators need to rethink the existing structure of the electric power industry because the continued application of traditional processes to its emerging competitive nature is creating a conflict between market-driven generators and regulated utilities, the Electric Generation Association says. Indeed, because of the current regulatory structure, many utilities have been forced to actively resist the development of a competitive market place, the group says in a paper published for this week's National Association of Regulatory Utility Commissioners winter meetings. In place of the existing structure, the industry needs a [open quotes]new, more discerning model of regulation[close quotes] that unbundles generation from transmission and realizes that, at least during the transition, all generation facilities are at risk of being considered stranded assets. A transition policy must minimize costs overall by achieving an early and smooth resolution of the stranded investment issue. One approach looks promising: Utilities that spin off high-cost assets would be preauthorized to enter into a binding contract to buy the output of the facility for an established period at rates slightly below what the cost of power would have been, assuming continued rate base treatment of the facility. Another alternative would reflect the rate design mechanisms used in the unbundling of gas supply from transportation service: A utility calculates the differential between the book value and market value of a high-cost asset, and then converts it from a generation-related charge into a form of transition surcharge. This is added to the inelastic portion of its system rates, which most logically is the distribution charge for retail and wholesale requirements customers. The charge would be applied over a specific period of time or to a specific volume of sales.

  4. Regulation and policy: International trends and issues

    SciTech Connect (OSTI)

    Griffin, W.S.

    1997-03-01

    As offshore oil and gas resources become exhausted, the associated production platforms and facilities will be decommissioned. The world-wide oil and gas industry is strictly regulated by global, regional and national guidelines which have been developed by governments to find the most responsible framework to perform the decommissioning. In the summer of 1995, the Brent Spar incident brought uncertainty to decommissioning world-wide. In June of 1995, a moratorium prohibiting sea disposal within the North East Atlantic was imposed by the Oslo Commission, and an unsuccessful attempt was made in December of 1995 to impose a world-wide moratorium on sea disposal at the London Convention.

  5. Self-regulating flow control device

    DOE Patents [OSTI]

    Humphreys, Duane A.

    1984-01-01

    A variable, self-regulating valve having a hydraulic loss coefficient proportional to a positive exponential power of the flow rate. The device includes two objects in a flow channel and structure which assures that the distance between the two objects is an increasing function of the flow rate. The range of spacing between the objects is such that the hydraulic resistance of the valve is an increasing function of the distance between the two objects so that the desired hydraulic loss coefficient as a function of flow rate is obtained without variation in the flow area.

  6. Environmental Regulation Impacts on Eastern Interconnection Performance

    SciTech Connect (OSTI)

    Markham, Penn N; Liu, Yilu; Young II, Marcus Aaron

    2013-07-01

    In the United States, recent environmental regulations will likely result in the removal of nearly 30 GW of oil and coal-fired generation from the power grid, mostly in the Eastern Interconnection (EI). The effects of this transition on voltage stability and transmission line flows have previously not been studied from a system-wide perspective. This report discusses the results of power flow studies designed to simulate the evolution of the EI over the next few years as traditional generation sources are replaced with environmentally friendlier ones such as natural gas and wind.

  7. RCRA Notification of Regulated Waste Activity (EPA Form 8700...

    Open Energy Info (EERE)

    Notification of Regulated Waste Activity (EPA Form 8700-12) Jump to: navigation, search OpenEI Reference LibraryAdd to library Form: RCRA Notification of Regulated Waste Activity...

  8. Colorado Parks and Wildlife Rules and Regulations | Open Energy...

    Open Energy Info (EERE)

    Rules and Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: Colorado Parks and Wildlife Rules and Regulations Abstract This web page lists...

  9. 5 CCR 1002-64 Biosolids Regulation | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- OtherOther: 5 CCR 1002-64 Biosolids RegulationLegal Abstract Regulations implementing the Colorado...

  10. North America: Regulation of International Electricity Trade | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy America: Regulation of International Electricity Trade North America: Regulation of International Electricity Trade North America: Regulation of International Electricity Trade prepared by North American Energy Working Group on December 2002. North America: Regulation of International Electricity Trade (68.28 KB) More Documents & Publications Regulatory Side-by-Side Governing Permitting of Cross-Border Electricity Transmission Facilities Between the United States and Canada

  11. EA-1631: Beacon Power Corporation Frequency Regulation Facility in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Stephentown, NY | Department of Energy 1: Beacon Power Corporation Frequency Regulation Facility in Stephentown, NY EA-1631: Beacon Power Corporation Frequency Regulation Facility in Stephentown, NY February 2, 2009 EA-1631: Final Environmental Assessment Loan Guarantee for Beacon Power Corporation Frequency Regulation Facility in Stephentown, New York February 27, 2009 EA-1631: Finding of No Significant Impact Loan Guarantee for Beacon Power Corporation Frequency Regulation Facility in

  12. QB1 - Stochastic Gene Regulation Munsky, Brian [Los Alamos National

    Office of Scientific and Technical Information (OSTI)

    FACILITIES; FLUCTUATIONS; GENE REGULATION; KINETICS; QUANTIZATION; SIMULATION; SWITCHES Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present...

  13. Contacts for the Assistant General Counsel for Legislation, Regulation, and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Efficiency | Department of Energy Legislation, Regulation, and Energy Efficiency Contacts for the Assistant General Counsel for Legislation, Regulation, and Energy Efficiency Office of the Assistant General Counsel for Legislation, Regulation & Energy Efficiency (GC-33) Daniel Cohen, Assistant General Counsel for Legislation, Regulation, & Energy Efficiency 202-586-9523 daniel.cohen@hq.doe.gov Catherine Goshe, Attorney-Adviser 202-586-7053 Preeti Chaudhari, Attorney-Adviser

  14. DOE Review of Regulations - Center for Regulatory Effectiveness Comments |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Review of Regulations - Center for Regulatory Effectiveness Comments DOE Review of Regulations - Center for Regulatory Effectiveness Comments The Center for Regulatory Effectiveness (CRE) is pleased to submit these comments to the Department of Energy (DOE) on how DOE can best review its existing regulations and to identify whether any of its existing regulations should be modified, streamlined, expanded or repealed. Center_for_Regulatory_Effectiveness_Comments.pdf

  15. DOE Revises its NEPA Regulations, Including Categorical Exclusions |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Revises its NEPA Regulations, Including Categorical Exclusions DOE Revises its NEPA Regulations, Including Categorical Exclusions September 30, 2011 - 2:30pm Addthis On September 27, 2011, the Department of Energy (DOE) approved revisions to its National Environmental Policy Act (NEPA) regulations, and on September 28th, submitted the revisions to the Federal Register. The final regulations, which become effective 30 days after publication in the Federal Register, are

  16. NARUC Releases Cybersecurity Primer for Utility Regulators (June 2012) |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy NARUC Releases Cybersecurity Primer for Utility Regulators (June 2012) NARUC Releases Cybersecurity Primer for Utility Regulators (June 2012) June 14, 2012 - 4:50pm Addthis The National Association of Regulatory Utility Commissioners (NARUC) has released "Cybersecurity for State Regulators," a primer that explains conceptual cybersecurity basics and points to additional resources that can help regulators develop internal cybersecurity expertise, ask questions

  17. Regulation of Viable and Optimal Cohorts

    SciTech Connect (OSTI)

    Aubin, Jean-Pierre

    2015-10-15

    This study deals with the evolution of (scalar) attributes (resources or income in evolutionary demography or economics, position in traffic management, etc.) of a population of “mobiles” (economic agents, vehicles, etc.). The set of mobiles sharing the same attributes is regarded as an instantaneous cohort described by the number of its elements. The union of instantaneous cohorts during a mobile window between two attributes is a cohort. Given a measure defining the number of instantaneous cohorts, the accumulation of the mobile attributes on a evolving mobile window is the measure of the cohort on this temporal mobile window. Imposing accumulation constraints and departure conditions, this study is devoted to the regulation of the evolutions of the attributes which are1.viable in the sense that the accumulations constraints are satisfied at each instant;2.and, among them, optimal, in the sense that both the duration of the temporal mobile window is maximum and that the accumulation on this temporal mobile window is the largest viable one. This value is the “accumulation valuation” function. Viable and optimal evolutions under accumulation constraints are regulated by an “implicit Volterra integro-differential inclusion” built from the accumulation valuation function, solution to an Hamilton–Jacobi–Bellman partial differential equation under constraints which is constructed for this purpose.

  18. Dancing with the regulations - Part Deux

    SciTech Connect (OSTI)

    Nitschke, R.L.

    1995-12-31

    The disposal of low-level radioactive waste (LLW) in the United States has long been subjected to two very similar regulations depending upon the location. Disposal sites located on Department of Energy (DOE) Reservations are subject to DOE Order 5820.2A {open_quotes}Radioactive Waste Management,{close_quotes} while disposal sites located elsewhere are subject to the Nuclear Regulatory Commission regulation 10 CFR 61 {open_quotes}Licensing Requirements for Land Disposal of Radioactive Waste.{close_quotes} While life was not necessarily good, there was only one sheet of music to dance to. Recently a new player, named CERCLA (Comprehensive Environmental Response, Compensation, and Liability Act), has ridden into those DOE towns, and for those whose disposal facilities lie within or adjacent to Superfund sites, she has brought along a different drum to dance to. This paper discusses the differences and similarities between the different dance partners and their associated musical scores (i.e., the performance assessment (PA) required by the DOE order and the baseline risk assessment (BRA) required by CERCLA). The paper then provides a brief discussion on the latest dancer to cut in: the Defense Nuclear Facilities Safety Board (DNFSB). This discussion should help to alleviate the confusion while dancing on the LLW disposal regulatory ballroom floor.

  19. cAMP-response-element-binding protein positively regulates breast cancer metastasis and subsequent bone destruction

    SciTech Connect (OSTI)

    Son, Jieun; Lee, Jong-Ho; Kim, Ha-Neui; Ha, Hyunil Lee, Zang Hee

    2010-07-23

    Research highlights: {yields} CREB is highly expressed in advanced breast cancer cells. {yields} Tumor-related factors such as TGF-{beta} further elevate CREB expression. {yields} CREB upregulation stimulates metastatic potential of breast cancer cells. {yields} CREB signaling is required for breast cancer-induced bone destruction. -- Abstract: cAMP-response-element-binding protein (CREB) signaling has been reported to be associated with cancer development and poor clinical outcome in various types of cancer. However, it remains to be elucidated whether CREB is involved in breast cancer development and osteotropism. Here, we found that metastatic MDA-MB-231 breast cancer cells exhibited higher CREB expression than did non-metastatic MCF-7 cells and that CREB expression was further increased by several soluble factors linked to cancer progression, such as IL-1, IGF-1, and TGF-{beta}. Using wild-type CREB and a dominant-negative form (K-CREB), we found that CREB signaling positively regulated the proliferation, migration, and invasion of MDA-MB-231 cells. In addition, K-CREB prevented MDA-MB-231 cell-induced osteolytic lesions in a mouse model of cancer metastasis. Furthermore, CREB signaling in cancer cells regulated the gene expression of PTHrP, MMPs, and OPG, which are closely involved in cancer metastasis and bone destruction. These results indicate that breast cancer cells acquire CREB overexpression during their development and that this CREB upregulation plays an important role in multiple steps of breast cancer bone metastasis.

  20. Liver X Receptor (LXR) activation negatively regulates visfatin expression in macrophages

    SciTech Connect (OSTI)

    Mayi, Therese Hervee; Rigamonti, Elena; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Pattou, Francois; Department of Endocrine Surgery, University Hospital, Lille; U859 Biotherapies for Diabetes, INSERM, Lille ; Staels, Bart; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille ; Chinetti-Gbaguidi, Giulia; INSERM UR1011, F-59000 Lille; UDSL, F-59000 Lille; Institut Pasteur de Lille, F-59019 Lille

    2011-01-07

    Research highlights: {yields} Synthetic LXR ligands decreased visfatin expression in human macrophages. {yields} LXR activation leads to a modest and transient decrease of NAD{sup +} concentration. {yields} LXR activation decreased PPAR{gamma}-induced visfatin in human macrophages. -- Abstract: Adipose tissue macrophages (ATM) are the major source of visfatin, a visceral fat adipokine upregulated during obesity. Also known to play a role in B cell differentiation (pre-B cell colony-enhancing factor (PBEF)) and NAD biosynthesis (nicotinamide phosphoribosyl transferase (NAMPT)), visfatin has been suggested to play a role in inflammation. Liver X Receptor (LXR) and Peroxisome Proliferator-Activated Receptor (PPAR){gamma} are nuclear receptors expressed in macrophages controlling the inflammatory response. Recently, we reported visfatin as a PPAR{gamma} target gene in human macrophages. In this study, we examined whether LXR regulates macrophage visfatin expression. Synthetic LXR ligands decreased visfatin gene expression in a LXR-dependent manner in human and murine macrophages. The decrease of visfatin mRNA was paralleled by a decrease of protein secretion. Consequently, a modest and transient decrease of NAD{sup +} concentration was observed. Interestingly, LXR activation decreased the PPAR{gamma}-induced visfatin gene and protein secretion in human macrophages. Our results identify visfatin as a gene oppositely regulated by the LXR and PPAR{gamma} pathways in human macrophages.

  1. Overpressure history of fractures, West Maracaibo Basin, Venezuela

    SciTech Connect (OSTI)

    Vrolijk, P.J.; Pottorf, R.J.; Maze, W.B.

    1996-08-01

    Prediction of density of natural fractures in reservoir rocks requires evaluation of many factors, including the effective stress and thus fluid pressure conditions through time. In this study we use analyses of fluid inclusions in calcite-filled fractures with burial and thermal history models to assess the fluid pressure history and the causes of overpressure in the West Maracaibo Basin. We analyzed abundant oil-filled and rare aqueous fluid inclusions in calcite-filled fractures in the La Luna Formation source rock and in the underlying Cogollo Gp. carbonate reservoir. Our analyses of fluid inclusion homogenization temperatures and fluorescence properties lead us to the interpretation of near-lithostatic paleo-fluid pressures in La Luna Formation and near-lithostatic to hydrostatic fluid pressures in the Cogollo, Gp. fractures. Maturation and expulsion of oil from the La Luna Formation source rock is required to generate the large inferred excess pressures as compaction disequilibrium and thermal expansion of pore fluids from rapid Miocene burial are insufficient to achieve near-lithostatic conditions. This hypothesis is supported by the observation of decreasing paleo- and modern fluid pressures with depth beneath the La Luna Formation. Thus based on the wide occurrence of oil-filled inclusions in calcite-filled fractures and the high fluid pressures associated with trapped oils, we infer extensive rock fracture under overpressured conditions near maximum Miocene burial, during inferred late source rock yield, and during Miocene growth of structural traps.

  2. Hydrocarbon segregation from well logs, Northern Monagas, Venezuela

    SciTech Connect (OSTI)

    Garcia, E. )

    1993-02-01

    A methodology is described to determine vertical hydrocarbon density variations in recent oil discoveries in Northern Monagas state, using well logs. Similarities and differences are established for models obtained in El Carito and El Tejero fault block-s. These models were confirmed using independent information, such as PVT analysis, RFT pressures, oil gravity and GOR's from initial production tests. To explain differences between the models, an hypothesis is proposed for the migration/segregation/deformation sequence in these two blocks, which accounts for the presence of lighter hydrocarbons in El Tejero block, even though it is 1700 ft structurally lower than El Carito. Based on this hypothesis, westward projection of the models predicts lighter hydrocarbons and similar porosities for Casupal-Mata Granda and Tonoro blocks, at depths considerably greater than in El Tejero block.

  3. Arsenic exposure disrupts epigenetic regulation of SIRT1 in human keratinocytes

    SciTech Connect (OSTI)

    Herbert, Katharine J.; Holloway, Adele; Cook, Anthony L.; Chin, Suyin P.; Snow, Elizabeth T.

    2014-11-15

    Arsenic is an environmental toxin which increases skin cancer risk for exposed populations worldwide; however the underlying biomolecular mechanism for arsenic-induced carcinogenesis is complex and poorly defined. Recent investigations show that histone deacetylase and DNA methyltransferase activity is impaired, and epigenetic patterns of gene regulation are consistently altered in cancers associated with arsenic exposure. Expression of the histone deacetylase SIRT1 is altered in solid tumours and haematological malignancies; however its role in arsenic-induced pathology is unknown. In this study we investigated the effect of arsenic on epigenetic regulation of SIRT1 and its targeting microRNA, miR-34a in primary human keratinocytes. Acetylation of histone H4 at lysine 16 (H4K16) increased in keratinocytes exposed to 0.5 μM arsenite [As(III)]; and this was associated with chromatin remodelling at the miR-34a promoter. Moreover, although SIRT1 protein initially increased in these As(III)-exposed cells, after 24 days expression was not significantly different from untreated controls. Extended exposure to low-dose As(III) (0.5 μM; > 5 weeks) compromised the pattern of CpG methylation at SIRT1 and miR-34a gene promoters, and this was associated with altered expression for both genes. We have found that arsenic alters epigenetic regulation of SIRT1 expression via structural reorganisation of chromatin at the miR-34a gene promoter in the initial 24 h of exposure; and over time, through shifts in miR-34a and SIRT1 gene methylation. Taken together, this investigation demonstrates that arsenic produces cumulative disruptions to epigenetic regulation of miR-34a expression, and this is associated with impaired coordination of SIRT1 functional activity. - Highlights: • Submicromolar arsenic concentrations disrupt SIRT1 activity and expression in human keratinocytes. • Arsenic-induced chromatin remodelling at the miR-34a gene promoter is associated with hyperacetylation

  4. Rac1 and Cdc42 GTPases regulate shear stress-driven ?-catenin signaling in osteoblasts

    SciTech Connect (OSTI)

    Wan, Qiaoqiao; Cho, Eunhye; Yokota, Hiroki; Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN 46202 ; Na, Sungsoo

    2013-04-19

    Highlights: Shear stress increased TCF/LEF activity and stimulated ?-catenin nuclear localization. Rac1, Cdc42, and RhoA displayed distinct dynamic activity patterns under flow. Rac1 and Cdc42, but not RhoA, regulate shear stress-driven TCF/LEF activation. Cytoskeleton did not significantly affect shear stress-induced TCF/LEF activation. -- Abstract: Beta-catenin-dependent TCF/LEF (T-cell factor/lymphocyte enhancing factor) is known to be mechanosensitive and an important regulator for promoting bone formation. However, the functional connection between TCF/LEF activity and Rho family GTPases is not well understood in osteoblasts. Herein we investigated the molecular mechanisms underlying oscillatory shear stress-induced TCF/LEF activity in MC3T3-E1 osteoblast cells using live cell imaging. We employed fluorescence resonance energy transfer (FRET)-based and green fluorescent protein (GFP)-based biosensors, which allowed us to monitor signal transduction in living cells in real time. Oscillatory (1 Hz) shear stress (10 dynes/cm{sup 2}) increased TCF/LEF activity and stimulated translocation of ?-catenin to the nucleus with the distinct activity patterns of Rac1 and Cdc42. The shear stress-induced TCF/LEF activity was blocked by the inhibition of Rac1 and Cdc42 with their dominant negative mutants or selective drugs, but not by a dominant negative mutant of RhoA. In contrast, constitutively active Rac1 and Cdc42 mutants caused a significant enhancement of TCF/LEF activity. Moreover, activation of Rac1 and Cdc42 increased the basal level of TCF/LEF activity, while their inhibition decreased the basal level. Interestingly, disruption of cytoskeletal structures or inhibition of myosin activity did not significantly affect shear stress-induced TCF/LEF activity. Although Rac1 is reported to be involved in ?-catenin in cancer cells, the involvement of Cdc42 in ?-catenin signaling in osteoblasts has not been identified. Our findings in this study demonstrate that both

  5. Irreversible inhibition of RANK expression as a possible mechanism for IL-3 inhibition of RANKL-induced osteoclastogenesis

    SciTech Connect (OSTI)

    Khapli, Shruti M.; Tomar, Geetanjali B.; Barhanpurkar, Amruta P.; Gupta, Navita; Yogesha, S.D.; Pote, Satish T.; Wani, Mohan R.

    2010-09-03

    Research highlights: {yields} IL-3 inhibits receptor activator of NF-{kappa}B ligand (RANKL)-induced osteoclastogenesis. {yields} IL-3 inhibits RANKL-induced JNK activation. {yields} IL-3 down-regulates expression of c-Fos and NFATc1 transcription factors. {yields} IL-3 down-regulates RANK expression posttranscriptionally and irreversibly. {yields} IL-3 inhibits in vivo RANK expression. -- Abstract: IL-3, a cytokine secreted by activated T lymphocytes, stimulates the proliferation, differentiation and survival of pluripotent hematopoietic stem cells. In this study, we investigated the mechanism of inhibitory action of IL-3 on osteoclast differentiation. We show here that IL-3 significantly inhibits receptor activator of NF-{kappa}B (RANK) ligand (RANKL)-induced activation of c-Jun N-terminal kinase (JNK). IL-3 down-regulates expression of c-Fos and nuclear factor of activated T cells (NFATc1) transcription factors. In addition, IL-3 down-regulates RANK expression posttranscriptionally in both purified osteoclast precursors and whole bone marrow cells. Furthermore, the inhibitory effect of IL-3 on RANK expression was irreversible. Interestingly, IL-3 inhibits in vivo RANK expression in mice. Thus, we provide the first evidence that IL-3 irreversibly inhibits RANK expression that results in inhibition of important signaling molecules induced by RANKL.

  6. Human mitochondrial transcription factor A functions in both nuclei and mitochondria and regulates cancer cell growth

    SciTech Connect (OSTI)

    Han, Bin; Izumi, Hiroto; Yasuniwa, Yoshihiro; Akiyama, Masaki; Yamaguchi, Takahiro; Fujimoto, Naohiro; Matsumoto, Tetsuro; Wu, Bin; Tanimoto, Akihide; Sasaguri, Yasuyuki; Kohno, Kimitoshi

    2011-04-29

    Highlights: {yields} Mitochondrial transcription factor A (mtTFA) localizes in nuclei and binds tightly to the nuclear chromatin. {yields} mtTFA contains two putative nuclear localization signals (NLS) in the HMG-boxes. {yields} Overexpression of mtTFA enhances the growth of cancer cells, whereas downregulation of mtTFA inhibits their growth by regulating mtTFA target genes, such as baculoviral IAP repeat-containing 5 (BIRC5; also known as survivin). {yields} Knockdown of mtTFA expression induces p21-dependent G1 cell cycle arrest. -- Abstract: Mitochondrial transcription factor A (mtTFA) is one of the high mobility group protein family and is required for both transcription from and maintenance of mitochondrial genomes. However, the roles of mtTFA have not been extensively studied in cancer cells. Here, we firstly reported the nuclear localization of mtTFA. The proportion of nuclear-localized mtTFA varied among different cancer cells. Some mtTFA binds tightly to the nuclear chromatin. DNA microarray and chromatin immunoprecipitation assays showed that mtTFA can regulate the expression of nuclear genes. Overexpression of mtTFA enhanced the growth of cancer cell lines, whereas downregulation of mtTFA inhibited their growth by regulating mtTFA target genes, such as baculoviral IAP repeat-containing 5 (BIRC5; also known as survivin). Knockdown of mtTFA expression induced p21-dependent G1 cell cycle arrest. These results imply that mtTFA functions in both nuclei and mitochondria to promote cell growth.

  7. Is anyone regulating naturally occurring radioactive material? A state survey

    SciTech Connect (OSTI)

    Gross, E.M.; Barisas, S.G.

    1993-08-01

    As far as we know, naturally occurring radioactive material (NORM) has surrounded humankind since the beginning of time. However, recent data demonstrating that certain activities concentrate NORM have increased concern regarding its proper handling and disposal and precipitated the development of new NORM-related regulations. The regulation of NORM affects the management of government facilities as well as a broad range of industrial processes. Recognizing that NORM regulation at the federal level is extremely limited, Argonne National Laboratory (ANL) conducted a 50-state survey to determine the extent to which states have assumed the responsibility for regulating NORM as well as the NORM standards that are currently being applied at the state level. Though the survey indicates that NORM regulation comprises a broad spectrum of controls from full licensing requirements to virtually no regulation at afl, a trend is emerging toward recognition of the need for increased regulation of potential NORM hazards, particularly in the absence of federal standards.

  8. ABT737 enhances cholangiocarcinoma sensitivity to cisplatin through regulation of mitochondrial dynamics

    SciTech Connect (OSTI)

    Fan, Zhongqi; Yu, Huimei; Cui, Ni; Kong, Xianggui; Liu, Xiaomin; Chang, Yulei; Wu, Yao; Sun, Liankun; Wang, Guangyi

    2015-07-01

    Cholangiocarcinoma responses weakly to cisplatin. Mitochondrial dynamics participate in the response to various stresses, and mainly involve mitophagy and mitochondrial fusion and fission. Bcl-2 family proteins play critical roles in orchestrating mitochondrial dynamics, and are involved in the resistance to cisplatin. Here we reported that ABT737, combined with cisplatin, can promote cholangiocarcinoma cells to undergo apoptosis. We found that the combined treatment decreased the Mcl-1 pro-survival form and increased Bak. Cells undergoing cisplatin treatment showed hyperfused mitochondria, whereas fragmentation was dominant in the mitochondria of cells exposed to the combined treatment, with higher Fis1 levels, decreased Mfn2 and OPA1 levels, increased ratio of Drp1 60 kD to 80 kD form, and more Drp1 located on mitochondria. More p62 aggregates were observed in cells with fragmented mitochondria, and they gradually translocated to mitochondria. Mitophagy was induced by the combined treatment. Knockdown p62 decreased the Drp1 ratio, increased Tom20, and increased cell viability. Our data indicated that mitochondrial dynamics play an important role in the response of cholangiocarcinoma to cisplatin. ABT737 might enhance cholangiocarcinoma sensitivity to cisplatin through regulation of mitochondrial dynamics and the balance within Bcl-2 family proteins. Furthermore, p62 seems to be critical in the regulation of mitochondrial dynamics. - Highlights: • Cholangiocarcinoma may adapt to cisplatin through mitochondrial fusion. • ABT737 sensitizes cholangiocarcinoma to cisplatin by promoting fission and mitophagy. • p62 might participate in the regulation of mitochondrial fission and mitophagy.

  9. When regulations go too far the taking of private property by environmental regulations

    SciTech Connect (OSTI)

    Plott, D.M.

    1995-12-01

    Seventy years ago Justice Oliver Wendell Homes wrote in an Opinion of the United States Supreme Court that{open_quotes}...while property may be regulated to a certain extent, if regulation goes too far it will be recognized as a taking.{close_quotes} Pennsylvania Coal Co. v. Mahon, 260 U.S. 393 (1992) The Fifth Amendment of the United States Constitution provides that private property shall not be {open_quotes}taken for public use, without just compensation{close_quotes}. After lying dormant for half a century, the {open_quotes}takings{close_quotes} clause of the United States constitution is being invoked to challenge environmental regulations throughout the country. In essence, property owners are saying that if the government wants to regulate to protect wetlands, wildlife habitat and other natural resources it must pay just compensation to the property owners for the restrictions imposed upon the use of their land. During the past ten years, the U.S. Supreme Court has decided several takings cases. While the judicial branch of government has become increasingly active in takings cases, the U.S. Congress and State legislatures have also been extremely active in efforts to protect private property rights. Typically, these legislative requires a takings impact analysis by the government to determine if its actions are likely to result in the compensable taking of private property or require compensation if property values are reduced by a certain percentage. This paper reviews the judicial standards that have been established for reviewing taking claims and sets forth a framework for analyzing when a regulation goes {open_quotes}too far{close_quotes} so as to require payment of compensation.

  10. System and method for regulating resonant inverters

    DOE Patents [OSTI]

    Stevanovic, Ljubisa Dragoljub (Clifton Park, NY); Zane, Regan Andrew (Superior, CO)

    2007-08-28

    A technique is provided for direct digital phase control of resonant inverters based on sensing of one or more parameters of the resonant inverter. The resonant inverter control system includes a switching circuit for applying power signals to the resonant inverter and a sensor for sensing one or more parameters of the resonant inverter. The one or more parameters are representative of a phase angle. The resonant inverter control system also includes a comparator for comparing the one or more parameters to a reference value and a digital controller for determining timing of the one or more parameters and for regulating operation of the switching circuit based upon the timing of the one or more parameters.

  11. Economics and regulation of petroleum futures markets

    SciTech Connect (OSTI)

    Not Available

    1980-08-01

    Because the futures market in petroleum products is a relatively recent phenomenon, the implications of public policies formulated for that market have not yet been fully explored. To provide the Office of Competition of the Department of Energy (DOE) with sufficient information to assess policy alternatives, Resource Planning Associates, Inc. (RPA) was asked to analyze the development of the futures market in No. 2 oil, assess the potential for futures markets in other petroleum products, and identify policy alternatives available to DOE. To perform this analysis, the criteria for a viable futures market was established first. Then, the experience to date with the 18-month-old futures market in No. 2 oil was examined, and the potential for viable futures markets in No. 6 oil, gasoline, jet fuel, and crude oil was assessed. Finally, how existing DOE regulations and prospective actions might affect petroleum futures market development was investigated.

  12. Body temperature regulation during euthermia and hyperthermia

    SciTech Connect (OSTI)

    Shimada, S.G.; Stitt, J.T.

    1981-10-01

    In this paper we have presented a few fundamental principles which are essential for an understanding of how microwaves might affect body temperature regulation. Application of the heat balance equation requires an accounting of all heat losses and heat gains. Thus, experimental measurements of these two processes are usually required to explain any thermoregulatory response. Knowledge of the individual elements of the thermoregulatory system and their integrative operation is imperative. Without such information one cannot hope to analyze or predict the consequences of microwave irradiation, or to separate simple heating effects from possible nonthermal effects. Finally, we have tried to emphasize that hyperthermia can be produced in different ways. Thus, there may be several forms of microwave hyperthermia, differing in their causality. Deposition of microwave energy in deep tissues might produce a response similar to exercise hyperthermia.

  13. Transcription regulation of the vegf gene by the BMP/Smad pathway in the angioblast of zebrafish embryos

    SciTech Connect (OSTI)

    He Chen; Chen Xiaozhuo . E-mail: chenx@ohiou.edu

    2005-04-01

    Vascular endothelial growth factor (VEGF) is a mitogen that is critically involved in vasculogenesis, angiogenesis, and hematopoiesis. However, what and how transcription factors participate in the regulation of vegf gene expression are not fully understood. Here we report the cloning and sequencing of the zebrafish vegf promoter which revealed that the promoter contains a number of bone morphogenetic protein (BMP)-activated Smad binding elements (SBE), implicating Smad1 and Smad5 in the regulation of BMP-induced expression of vegf. Electrophoretic mobility shift assays of adding recombinant Smad proteins to the SBE-containing DNA oligonucleotides that represent portions of zebrafish vegf promoter resulted in mobility shift of the oligonucleotides. These changes demonstrate potential interactions between Smad1/5 and the vegf promoter. Reporter activity assays using the wild-type or SBE-deleted vegf promoters to drive the luciferase reporter gene expression revealed that Smad1 stimulated while Smad5 repressed the vegf promoter activity in zebrafish embryos. These data indicate that the BMP/Smad signaling pathway is involved in the regulation of zebrafish vegf transcription. In addition, we demonstrate that transgenic expression of human BMP4 in zebrafish embryos induced an expansion of the posterior intermediate cell mass (ICM, also commonly called blood island), a population of cells containing endothelial and hematopoietic precursors. In the expanded ICM, vegf and VEGF receptor 2 (flk-1) were ectopically co-expressed, suggesting that an autocrine/paracrine regulation of vegf expression may exist and contribute to the BMP-induced hemangiogenic cell proliferation.

  14. PES1 regulates sensitivity of colorectal cancer cells to anticancer drugs

    SciTech Connect (OSTI)

    Xie, Wei; Qu, Like; Meng, Lin; Liu, Caiyun; Wu, Jian; Shou, Chengchao

    2013-02-15

    Highlights: ? PES1 was overexpressed in diverse cancer cell lines. ? PES1-ablation enhances DNA damage response by decreasing DNA repair. ? PES1-ablation increases the sensitivity of HCT116 cells to chemotherapeutic agents. ? PES1-ablation is associated with diminished nuclear entry of RAD51. -- Abstract: PES1 (also known as Pescadillo), a nucleolar protein, was involved in biogenesis of ribosomal RNA. Up-regulation of PES1 has been documented in some human cancers, indicating that PES1 may play some crucial roles in tumorigenesis. In our previous study, it was found that silencing of PES1 resulted in decreased proliferation of colorectal cancer cells. We also noticed that depletion of PES1 altered expression profiles of diverse genes. In the present study, we validated the expression changes of a subset of genotoxic stress-related genes in PES1-silenced HCT116 cells by quantitative RT-PCR. The steady and etoposide-induced phosphorylated H2AX (?-H2AX) were higher in PES1-silenced cells than in control cells. Besides, etoposide-induced ?-H2AX persisted longer in PES1-silenced cells after removing the etoposide. Next, results of comet assay revealed decreased DNA repair after PES1-ablation. PES1-ablated cells were more sensitive to chemotherapeutic agents, which could be reversed by reconstitution with exogenous PES1. Furthermore, deletion of PES1 diminished steady and DNA damage-induced levels of nuclear RAD51. Our results uncover a potential role of PES1 in chemoresistance by regulating DNA damage response in colorectal cancer cells.

  15. Ginsenoside Rg3 regulates S-nitrosylation of the NLRP3 inflammasome via suppression of iNOS

    SciTech Connect (OSTI)

    Yoon, Sung-Jin; Park, Jun-Young; Choi, Song; Lee, Jin-Bong; Jung, Haiyoung; Kim, Tae-Don; Yoon, Suk Ran; Choi, Inpyo; Shim, Sungbo; Park, Young-Jun

    2015-08-07

    Ginsenoside Rg3, a specific biological effector, is well-known as a major bioactive ingredient of Panax ginseng. However, its role in the inflammasome activation process remains unclear. In this report, we demonstrate that ginsenosides 20(R)-Rg3 and 20(S)-Rg3 are capable of suppressing both lethal endotoxic shock and the S-nitrosylation of the NLRP3 inflammasome by inhibiting nitric oxide (NO) production through the regulation of inducible nitric oxide synthase (iNOS) expression. In response to lipopolysaccharide (LPS), the reducing effect of 20(S)-Rg3 and 20(R)-Rg3 on nitric oxide led to an increase in the survival time of mice after lethal endotoxin-induced shock, and excess levels of NO inhibited IL-1β production via the S-nitrosylation of the NLRP3 inflammasome. In addition, ginsenosides 20(R)-Rg3 and 20(S)-Rg3 had suppressive effects on the LPS- or UV-irradiation-induced reactive oxygen species (ROS) levels in macrophage and HaCaT cells and thereby prevented apoptosis of spleen cells in mice. Altogether, these results demonstrate that ginsenoside 20(R)-Rg3 and 20(S)-Rg3, a naturally occurring compound, might act as a dual therapeutic regulator for the treatment of inflammatory and oxidative stress-related diseases. - Highlights: • Ginsenosides Rg3 inhibits NO production through the regulation of iNOS expression. • Ginsenosides Rg3 inhibits the S-nitrosylation of the NLRP3 inflammasome. • Ginsenosides Rg3 suppress on the LPS- or UV-irradiation-induced ROS levels in cells.

  16. Nickel-regulated heart rate variability: The roles of oxidative stress and inflammation

    SciTech Connect (OSTI)

    Chuang, Hsiao-Chi; Hsueh, Tzu-Wei; Chang, Chuen-Chau; Hwang, Jing-Shiang; Chuang, Kai-Jen; Yan, Yuan-Horng; Cheng, Tsun-Jen

    2013-01-15

    Heart rate variability (HRV) has been reported to be a putative marker of cardiac autonomic imbalance caused by exposure to ambient particulate matter (PM). Our objective in this study was to determine the effects on HRV from exposure to nickel, an important chemical component of ambient PM that results in oxidative stress and inflammation. HRV data were collected for 72 h before lung exposure (baseline) and 72 h after intratracheal exposure (response) to nickel sulphate (NiSO{sub 4}; 526 μg) in Wistar Kyoto (WKY) and spontaneously hypertensive (SH) rats. The antioxidant N-acetyl-L-cysteine (NAC) and the anti-inflammatory celecoxib were intraperitoneally injected to examine post-exposure oxidative and inflammatory responses. Self-controlled experiments examined the effects of NiSO{sub 4} exposure on average normal-to-normal intervals (ANN), natural logarithm-transformed standard deviation of the normal-to-normal intervals (LnSDNN) and root mean square of successive differences of adjacent normal-to-normal intervals (LnRMSSD); the resulting data were sequentially analysed using the generalised estimating equation model. HRV effects on NiSO{sub 4}-exposed SH rats were greater than those on NiSO{sub 4}-exposed WKY rats. After adjusted the HRV responses in the WKY rats as control, ANN and LnRMSSD were found to be quadratically increased over 72 h after exposure to NiSO{sub 4}. Both NAC and celecoxib mitigated the NiSO{sub 4}-induced alterations in HRV during the exposure period. The results suggest that concurrent Ni-induced oxidative stress and inflammatory responses play important roles in regulating HRV. These findings help bridge the gap between epidemiological and clinical studies on the plausible mechanisms of the cardiovascular consequences induced by chemical components in ambient PM. -- Highlights: ► To determine the effects on HRV from exposure to nickel. ► ANN and LnRMSSD were found to be quadratically increased after exposure to Ni. ► NAC and

  17. Nickel differentially regulates NFAT and NF-{kappa}B activation in T cell signaling

    SciTech Connect (OSTI)

    Saito, Rumiko; Hirakawa, Satoshi; Ohara, Hiroshi; Yasuda, Makoto; Yamazaki, Tomomi; Nishii, Shigeaki; Aiba, Setsuya

    2011-08-01

    Nickel is a potent hapten that induces contact hypersensitivity in human skin. While nickel induces the maturation of dendritic cells via NF-{kappa}B and p38 MAPK activation, it also exerts immunosuppressive effects on T cells through an unknown mechanism. To elucidate the molecular mechanisms of its effects on T cells, we examined the effects of NiCl{sub 2} on mRNA expression in human CD3+ T cells stimulated with CD3 and CD28 antibodies. Using a DNA microarray and Gene Ontology, we identified 70 up-regulated (including IL-1{beta}, IL-6 and IL-8) and 61 down-regulated (including IL-2, IL-4, IL-10 and IFN-{gamma}) immune responsive genes in NiCl{sub 2}-treated T cells. The DNA microarray results were verified using real-time PCR and a Bio-Plex{sup TM} suspension protein array. Suppression of IL-2 and IFN-{gamma} gene transcription by NiCl{sub 2} was also confirmed using Jurkat T cells transfected with IL-2 or IFN-{gamma} luciferase reporter genes. To explore the NiCl{sub 2}-regulated signaling pathway, we examined the binding activity of nuclear proteins to NFAT, AP-1, and NF-{kappa}B consensus sequences. NiCl{sub 2} significantly and dose-dependently suppressed NFAT- and AP-1-binding activity, but augmented NF-{kappa}B-binding activity. Moreover, NiCl{sub 2} decreased nuclear NFAT expression in stimulated T cells. Using Jurkat T cells stimulated with PMA/ionomycin, we demonstrated that NiCl{sub 2} significantly suppressed stimulation-evoked cytosolic Ca{sup 2+} increases, suggesting that NiCl{sub 2} regulates NFAT signals by acting as a blocker of Ca{sup 2+} release-activated Ca{sup 2+} (CRAC) channels. These data showed that NiCl{sub 2} decreases NFAT and increases NF-{kappa}B signaling in T cells. These results shed light on the effects of nickel on the molecular regulation of T cell signaling. - Graphical Abstract: Nickel suppresses stimulation-evoked cytosolic Ca{sup 2+} increase, which results in the suppression of NFAT signals. On the other hand, Ni rather

  18. Genetic analysis of the regulation of TCH gene expression, Final Report

    SciTech Connect (OSTI)

    Braam, Janet

    2008-10-28

    The Arabidopsis TCH genes, originally isolated as a consequence of their upregulation in response to the mechanical stimulus of touch, are also upregulated by a variety of seemingly disparate environmental and hormonal stimuli. To gain insight into the complexities of TCH gene regulation, a number of approaches were taken. Regulatory elements responsible for regulation were identified and characteristics of the regulation were evaluated. Reporter genes were used to monitor expression localization and dynamics. Microarray analyses of genome-wide expression behavior indicated that touch-inducible gene expression is more widespread than generally appreciated. Identification of all touch-regulated genes shed light on the types of cellular processes that may be altered in response to mechanical stress perturbations. Expression of the TCH2 gene, also called CML24, encoding a calmodulin (CaM)-like (CML) protein, was evaluated. CML24 shares over 40% amino acid sequence identity with CaM, has 4 EF hands and undergoes a Ca2+-dependent change in migration rate through denaturing gel electrophoresis, indicating that CML24 binds Ca2+ and, as a consequence, undergoes conformational changes. CML24 expression occurs in all major organs and is induced from 2- to 15-fold in plants subjected to touch, darkness, heat, cold, hydrogen peroxide, abscisic acid (ABA) and indole-3-acetic acid. The putative CML24 regulatory region confers reporter expression at sites of predicted mechanical stress, in regions undergoing growth, in vascular tissues and various floral organs and in stomata, trichomes and hydathodes. CML24 underexpressing transgenics are resistant to ABA inhibition of germination and seedling growth, defective in long-day induction of flowering, and have enhanced tolerance to CoCl2, molybdic acid, ZnSO4 and MgCl2. These data present evidence that CML24 encodes a potential Ca2+ sensor that may function to enable responses to ABA, day length and presence of various salts. Further

  19. Colorado geothermal institutional handbook: a user's guide of agencies regulations, permits, and aids for geothermal development

    SciTech Connect (OSTI)

    Coe, B.A.; Forman, N.A.

    1980-01-01

    The following are included: principal state agencies, applicable state legislation, applicable state regulations, local agencies and regulations, federal agencies and regulations, information sources, and agencies and individuals. (MHR)

  20. Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina

    SciTech Connect (OSTI)

    Tokuda, Kazuhiro; Kuramitsu, Yasuhiro; Byron, Baron; Kitagawa, Takao; Tokuda, Nobuko; Kobayashi, Daiki; Nagayama, Megumi; Araki, Norie; Sonoda, Koh-Hei; Nakamura, Kazuyuki

    2015-08-07

    Glutamate has been shown to induce neural progenitor cells in the adult vertebrate retina. However, protein dynamics during progenitor cell induction by glutamate are not fully understood. To identify specific proteins involved in the process, we employed two-dimensional electrophoresis-based proteomics on glutamate untreated and treated retinal ex vivo sections. Rat retinal tissues were incubated with 1 mM glutamate for 1 h, followed by incubation in glutamate-free media for a total of 24 h. Consistent with prior reports, it was found that mitotic cells appeared in the outer nuclear layer without any histological damage. Immunohistological evaluations and immunoblotting confirmed the emergence of neuronal progenitor cells in the mature retina treated with glutamate. Proteomic analysis revealed the up-regulation of dihydropyrimidinase-related protein 3 (DRP-3), DRP-2 and stress-induced-phosphoprotein 1 (STIP1) during neural progenitor cell induction by glutamate. Moreover, mRNA expression of DRP-3, especially, its long isoform, robustly increased in the treated retina compared to that in the untreated retina. These results may indicate that glutamate induces neural progenitor cells in the mature rat retina by up-regulating the proteins which mediate cell mitosis and neurite growth. - Highlights: • Glutamate induced neuronal progenitor cells in the mature rat retina. • Proteomic analysis revealed the up-regulation of DRP-3, DRP-2 and STIP1. • mRNA expression of DRP-3, especially, its long isoform, robustly increased.

  1. P70S6K 1 regulation of angiogenesis through VEGF and HIF-1{alpha} expression

    SciTech Connect (OSTI)

    Bian, Chuan-Xiu; Shi, Zhumei; Meng, Qiao; Jiang, Yue; Liu, Ling-Zhi; Jiang, Bing-Hua; Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107

    2010-07-30

    Research highlights: {yields} P70S6K1 regulates VEGF expression; {yields} P70S6K1 induces transcriptional activation through HIF-1{alpha} binding site; {yields} P70S6K1 regulates HIF-1{alpha}, but not HIF-1{beta} protein expression; {yields} P70S6K1 mediates tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression. -- Abstract: The 70 kDa ribosomal S6 kinase 1 (p70S6K1), a downstream target of phosphoinositide 3-kinase (PI3K) and ERK mitogen-activated protein kinase (MAPK), is an important regulator of cell cycle progression, and cell proliferation. Recent studies indicated an important role of p70S6K1 in PTEN-negative and AKT-overexpressing tumors. However, the mechanism of p70S6K1 in tumor angiogenesis remains to be elucidated. In this study, we specifically inhibited p70S6K1 activity in ovarian cancer cells using vector-based small interfering RNA (siRNA) against p70S6K1. We found that knockdown of p70S6K1 significantly decreased VEGF protein expression and VEGF transcriptional activation through the HIF-1{alpha} binding site at its enhancer region. The expression of p70S6K1 siRNA specifically inhibited HIF-1{alpha}, but not HIF-1{beta} protein expression. We also found that p70S6K1 down-regulation inhibited ovarian tumor growth and angiogenesis, and decreased cell proliferation and levels of VEGF and HIF-1{alpha} expression in tumor tissues. Our results suggest that p70S6K1 is required for tumor growth and angiogenesis through HIF-1{alpha} and VEGF expression, providing a molecular mechanism of human ovarian cancer mediated by p70S6K1 signaling.

  2. JAZF1 can regulate the expression of lipid metabolic genes and inhibit lipid accumulation in adipocytes

    SciTech Connect (OSTI)

    Ming, Guang-feng; Xiao, Di; Gong, Wei-jing; Liu, Hui-xia; Liu, Jun; Zhou, Hong-hao; Liu, Zhao-qian

    2014-03-14

    Highlights: • JAZF1 was significantly upregulated during the differentiation of 3T3-L1 preadipocytes. • JAZF1 overexpression inhibited lipid accumulation in differentiated mature 3T3-L1 adipocytes. • JAZF1 overexpression inhibited the expression of SREBP1, ACC, and FAS. • JAZF1 overexpression upregulated the expression of HSL and ATGL. • SREBP1 and JAZF1 could regulate each other in adipocytes. - Abstract: JAZF1 is a newly identified gene with unknown functions. A recent genome-wide association study showed that JAZF1 is associated with type 2 diabetes and is highly expressed in liver and adipose tissue. Studies have demonstrated that JAZF1 is the co-repressor for nuclear orphan receptor TAK1, whereas most nuclear orphan receptor family members are involved in the regulation of lipid metabolism. Therefore, JAZF1 could be closely related to glycolipid metabolism. In this study, JAZF1 was significantly upregulated during the induced differentiation process of 3T3-L1 preadipocytes. The overexpression of JAZF1 inhibited lipid accumulation in differentiated mature 3T3-L1 adipocytes and significantly inhibited the expression of SREBPl, ACC, and FAS, which were important in lipid synthesis, while upregulating the expression of key enzyme hormone-sensitive lipase in lipoclasis. Moreover, SREBPl exhibited an inhibitory function on the expression of JAZF1. SREBP1 reversed the inhibitory action on lipid accumulation of JAZF1. SREBP1 and JAZF1 were observed to regulate each other in adipocytes. Therefore, JAZF1 could regulate the expression of particular genes related to lipid metabolism and inhibit lipid accumulation in adipocytes. This result suggests that JAZF1 may be a potential target for the treatment of diseases, such as obesity and lipid metabolism disorders.

  3. Up-regulation of K{sub ir}2.1 by ER stress facilitates cell death of brain capillary endothelial cells

    SciTech Connect (OSTI)

    Kito, Hiroaki; Yamazaki, Daiju; Department of Biological Chemistry, Kyoto University, Graduate School of Pharmaceutical Sciences, Kyoto; Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Nagoya ; Ohya, Susumu; Yamamura, Hisao; Asai, Kiyofumi; Imaizumi, Yuji

    2011-07-29

    Highlights: {yields} We found that application of endoplasmic reticulum (ER) stress with tunicamycin to brain capillary endothelial cells (BCECs) induced cell death. {yields} The ER stress facilitated the expression of inward rectifier K{sup +} channel (K{sub ir}2.1) and induced sustained membrane hyperpolarization. {yields} The membrane hyperpolarization induced sustained Ca{sup 2+} entry through voltage-independent nonspecific cation channels and consequently facilitated cell death. {yields} The K{sub ir}2.1 up-regulation by ER stress is, at least in part, responsible for cell death of BCECs under pathological conditions. -- Abstract: Brain capillary endothelial cells (BCECs) form blood brain barrier (BBB) to maintain brain homeostasis. Cell turnover of BCECs by the balance of cell proliferation and cell death is critical for maintaining the integrity of BBB. Here we found that stimuli with tunicamycin, endoplasmic reticulum (ER) stress inducer, up-regulated inward rectifier K{sup +} channel (K{sub ir}2.1) and facilitated cell death in t-BBEC117, a cell line derived from bovine BCECs. The activation of K{sub ir} channels contributed to the establishment of deeply negative resting membrane potential in t-BBEC117. The deep resting membrane potential increased the resting intracellular Ca{sup 2+} concentration due to Ca{sup 2+} influx through non-selective cation channels and thereby partly but significantly regulated cell death in t-BBEC117. The present results suggest that the up-regulation of K{sub ir}2.1 is, at least in part, responsible for cell death/cell turnover of BCECs induced by a variety of cellular stresses, particularly ER stress, under pathological conditions.

  4. The fluctuation induced Hall effect

    SciTech Connect (OSTI)

    Shen, W.; Prager, S.C.

    1993-02-01

    The fluctuation induced Hall term, [le][approximately][ovr J] [times] [approximately][ovr B][ge], has been measured in the MST reversed field pinch. The term is of interest as a possible source of current self-generation (dynamo). It is found to be non-negligible, but small in that it can account for less than 25% of the dynamo driven current.

  5. The fluctuation induced Hall effect

    SciTech Connect (OSTI)

    Shen, W.; Prager, S.C.

    1993-02-01

    The fluctuation induced Hall term, {le}{approximately}{ovr J} {times} {approximately}{ovr B}{ge}, has been measured in the MST reversed field pinch. The term is of interest as a possible source of current self-generation (dynamo). It is found to be non-negligible, but small in that it can account for less than 25% of the dynamo driven current.

  6. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    induced incandescence (LII) - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced

  7. Incentive regulation of investor-owned nuclear power plants by public utility regulators. Revision 1

    SciTech Connect (OSTI)

    McKinney, M.D.; Seely, H.E.; Merritt, C.R.; Baker, D.C.

    1995-04-01

    The US Nuclear Regulatory Commission (NRC) periodically surveys the Federal Energy Regulatory Commission (FERC) and state regulatory commissions that regulate utility owners of nuclear power plants. The NRC is interested in identifying states that have established economic or performance incentive programs applicable to nuclear power plants, how the programs are being implemented, and in determining the financial impact of the programs on the utilities. The NRC interest stems from the fact that such programs have the potential to adversely affect the safety of nuclear power plants. The current report is an update of NUREG/CR-5975, Incentive Regulation of Investor-Owned Nuclear Power Plants by Public Utility Regulators, published in January 1993. The information in this report was obtained from interviews conducted with each state regulatory agency that administers an incentive program and each utility that owns at least 10% of an affected nuclear power plant. The agreements, orders, and settlements that form the basis for each incentive program were reviewed as required. The interviews and supporting documentation form the basis for the individual state reports describing the structure and financial impact of each incentive program.

  8. INPP4B-mediated tumor resistance is associated with modulation of glucose metabolism via hexokinase 2 regulation in laryngeal cancer cells

    SciTech Connect (OSTI)

    Min, Joong Won; Kim, Kwang Il; Kim, Hyun-Ah; Kim, Eun-Kyu; Noh, Woo Chul; Jeon, Hong Bae; Cho, Dong-Hyung; Oh, Jeong Su; Park, In-Chul; Hwang, Sang-Gu; Kim, Jae-Sung

    2013-10-11

    Highlights: •HIF-1α-regulated INPP4B enhances glycolysis. •INPP4B regulates aerobic glycolysis by inducing HK2 via Akt-mTOR pathway. •Blockage of INPP4B and HK2 sensitizes radioresistant laryngeal cancer cells to radiation and anticancer drug. •INPP4B is associated with HK2 in human laryngeal cancer tissues. -- Abstract: Inositol polyphosphate 4-phosphatase type II (INPP4B) was recently identified as a tumor resistance factor in laryngeal cancer cells. Herein, we show that INPP4B-mediated resistance is associated with increased glycolytic phenotype. INPP4B expression was induced by hypoxia and irradiation. Intriguingly, overexpression of INPP4B enhanced aerobic glycolysis. Of the glycolysis-regulatory genes, hexokinase 2 (HK2) was mainly regulated by INPP4B and this regulation was mediated through the Akt-mTOR pathway. Notably, codepletion of INPP4B and HK2 markedly sensitized radioresistant laryngeal cancer cells to irradiation or anticancer drug. Moreover, INPP4B was significantly associated with HK2 in human laryngeal cancer tissues. Therefore, these results suggest that INPP4B modulates aerobic glycolysis via HK2 regulation in radioresistant laryngeal cancer cells.

  9. Environmental controls: Market incentives v. direct regulation

    SciTech Connect (OSTI)

    Kosobud, R.F.; Atallah, D.S.

    1996-12-31

    Cap-and-trade environmental markets, where the commodities are tradable pollution rights, are being introduced in several closely watched applications as a potentially more cost-effective way of cleaning up the environment than direct or command-and-control (CAC) regulation. In this study, we examine the evidence on control cost savings provided by price and transactions data from the first few years of activity in two markets designed to reduce atmospheric pollution. Some observers of both markets have argued that prices for tradable permits lower than expected, and transactions fewer than expected, are evidence that the markets are not achieving the hoped for savings. It was found, on the contrary, that observed prices point toward more flexible and improved pollution control choices and that the number of transactions has been steadily increasing as market incentives are incorporated into enterprise decisions. These new markets during their first few years are generating, according to our estimates, control cost savings in the neighborhood of one to two billion dollars annually. However, there is evidence that the markets have not yet reached their full potential. In the course of this study, several obstacles to market performance were found that are worthy of attention by policy makers. 13 refs., 4 figs., 1 tab.

  10. Natural Gas Regulation - Other Gas-Related Information Sources | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Natural Gas Regulation - Other Gas-Related Information Sources Natural Gas Regulation - Other Gas-Related Information Sources The single largest source of energy information available is the Department of Energy's Energy Information Administration (EIA). The EIA publishes extensive reports on natural gas and other energy sources. Domestic natural gas markets are regulated in part by the Federal Energy Regulatory Commission. The commission's chief area of concern is the interstate

  11. Code of Federal Regulations NUCLEAR SAFETY MANAGEMENT | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy NUCLEAR SAFETY MANAGEMENT Code of Federal Regulations NUCLEAR SAFETY MANAGEMENT This part governs the conduct of DOE contractors, DOE personnel, and other persons conducting activities (including providing items and services) that affect, or may affect, the safety of DOE nuclear facilities. Code of Federal Regulations NUCLEAR SAFETY MANAGEMENT (167.52 KB) More Documents & Publications Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY Code of Federal

  12. Key Publications - Natural Gas Regulation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Services » Natural Gas Regulation » Key Publications - Natural Gas Regulation Key Publications - Natural Gas Regulation Natural Gas Imports and Exports - Quarterly Reports May 11, 2016 Natural Gas Imports and Exports Fourth Quarter Report 2015 February 3, 2016 Natural Gas Imports and Exports Third Quarter Report 2015 October 30, 2015 Natural Gas Imports and Exports Second Quarter Report 2015 August 13, 2015 Natural Gas Imports and Exports First Quarter Report 2015 More Quarterly Reports LNG

  13. Regulations, Guidelines and Codes and Standards | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Current Approaches to Safety, Codes & Standards » Regulations, Guidelines and Codes and Standards Regulations, Guidelines and Codes and Standards Many regulations, guidelines, and codes and standards have already been established through years of hydrogen use in industrial and aerospace applications. In addition, systems and organizations are already in place to establish codes and standards that facilitate hydrogen and fuel cell commercialization. Standards Development Organizations

  14. Harmonization of Federal and International Regulations | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Harmonization of Federal and International Regulations Harmonization of Federal and International Regulations Update of the U.S. Department of Transportation (DOT) Pipeline and Hazardous Materials Safety Administration (PHMSA). Harmonization of Federal and International Regulations (636.69 KB) More Documents & Publications DOE-STD-5507-2013 Department of Transportation Pipeline and Hazardous Materials Safety Administration Activities FAQS Reference Guide - NNSA Package

  15. Contacts for the Deputy General Counsel for Litigation, Regulation and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Enforcement (GC-30) | Department of Energy Litigation, Regulation and Enforcement (GC-30) Contacts for the Deputy General Counsel for Litigation, Regulation and Enforcement (GC-30) Anne Harkavy, Deputy General Counsel for Litigation, Regulation, and Enforcement 202-586-8700 anne.harkavy@hq.doe.gov Margie L. Washington, Administrative Support Specialist 202-586-8700 202-586-3437 (fax) margie.washington@hq.doe.gov

  16. Environmental Regulations and Changes in Petroleum Refining Operations

    Gasoline and Diesel Fuel Update (EIA)

    Environmental Regulations and Changes in Petroleum Refining Operations By Tancred C.M. Lidderdale Contents * Introduction * Motor Gasoline Summer Volatility (RVP) Regulations o Table 1. Summer Volatility Regulations for Motor Gasoline o Table 2. Refinery Inputs and Production of Normal Butane o Figure 1. Refinery Inputs and Production of Normal Butane o Table 3. Price Relationship Between Normal Butane and Motor Gasoline o Table 4. Market Price Premium for Low Vapor Pressure (RVP) Gasoline *

  17. Utility Regulation and Business Model Reforms for Addressing the Financial

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Impacts of Distributed Solar on Utilities | Department of Energy Utility Regulation and Business Model Reforms for Addressing the Financial Impacts of Distributed Solar on Utilities Utility Regulation and Business Model Reforms for Addressing the Financial Impacts of Distributed Solar on Utilities Utility Regulation and Business Model Reforms for Addressing the Financial Impacts of Distributed Solar on Utilities Implementing a range of alternative utility-rate reforms could minimize solar

  18. Vehicle Technologies Office: Regulated Fleets | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Alternative Fuels » Vehicle Technologies Office: Regulated Fleets Vehicle Technologies Office: Regulated Fleets The Office of Energy Efficiency and Renewable Energy (EERE) manages several programs designed to fulfill the requirements of the original and amended versions of the Energy Policy Act of 1992 (EPAct) that regulate and guide specific types of fleets with the goal of reducing the United States' petroleum consumption. EERE's Vehicle Technologies Office (VTO) implements the EPAct

  19. Building Codes and Regulations for Solar Water Heating Systems | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Codes and Regulations for Solar Water Heating Systems Building Codes and Regulations for Solar Water Heating Systems Photo Credit: iStockphoto Photo Credit: iStockphoto Before installing a solar water heating system, you should investigate local building codes, zoning ordinances, and subdivision covenants, as well as any special regulations pertaining to the site. You will probably need a building permit to install a solar energy system onto an existing building. Not every

  20. Office of the Assistant General Counsel for Legislation, Regulation and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Efficiency | Department of Energy Legislation, Regulation and Energy Efficiency Office of the Assistant General Counsel for Legislation, Regulation and Energy Efficiency The Office of the Assistant General Counsel for Legislation, Regulation and Energy Efficiency (GC-33) provides legal support and advice on legislative matters throughout the Department. It participates in the development of DOE views on pending legislation, drafts DOE legislative proposals and supporting documents,

  1. Department of Energy Acquisition Regulation (DEAR) Final Rule for changes

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    to Parts 908, 945, 952, and 970 regarding Government Property | Department of Energy Energy Acquisition Regulation (DEAR) Final Rule for changes to Parts 908, 945, 952, and 970 regarding Government Property Department of Energy Acquisition Regulation (DEAR) Final Rule for changes to Parts 908, 945, 952, and 970 regarding Government Property Department of Energy Acquisition Regulation (DEAR) Final Rule for changes to Parts 908, 945, 952, and 970 regarding Government Property was published

  2. Department of Energy Finalizes Regulations to Increase Energy Efficiency in

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    New Federal Buildings by 30% | Department of Energy Regulations to Increase Energy Efficiency in New Federal Buildings by 30% Department of Energy Finalizes Regulations to Increase Energy Efficiency in New Federal Buildings by 30% December 21, 2007 - 4:58pm Addthis WASHINGTON DC - The U.S. Department of Energy (DOE) today announced it has established regulations that require new Federal buildings to achieve at least 30% greater energy efficiency over prevailing building codes. Mandated by

  3. Code of Federal Regulations OCCUPATIONAL RADIATION PROTECTION | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy OCCUPATIONAL RADIATION PROTECTION Code of Federal Regulations OCCUPATIONAL RADIATION PROTECTION The rules in this part establish radiation protection standards, limits, and program requirements for protecting individuals from ionizing radiation resulting from the conduct of DOE activities. Code of Federal Regulations OCCUPATIONAL RADIATION PROTECTION (329.58 KB) More Documents & Publications Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION Subpart A -

  4. Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY The regulations in this part are issued for the protection and security of facilities, installations and real property subject to the jurisdiction or administration, or in the custody of, the Department of Energy. Code of Federal Regulations TRESPASSING ON DEPARTMENT OF ENERGY PROPERTY (171.25 KB) More Documents & Publications Code of Federal

  5. UAP56 is a novel interacting partner of Bcr in regulating vascular smooth muscle cell DNA synthesis

    SciTech Connect (OSTI)

    Sahni, Abha; Wang, Nadan; Alexis, Jeffrey D.

    2012-04-13

    Highlights: Black-Right-Pointing-Pointer UAP56 is an important regulator of DNA synthesis in vascular smooth muscle cells. Black-Right-Pointing-Pointer UAP56 binds to Bcr. Black-Right-Pointing-Pointer Interaction between Bcr and UAP56 is critical for Bcr induced DNA synthesis. -- Abstract: Bcr is a serine/threonine kinase that is a critical regulator of vascular smooth muscle cell inflammation and proliferation. We have previously demonstrated that Bcr acts in part via phosphorylation and inhibition of PPAR{gamma}. We have identified the RNA helicase UAP56 as another substrate of Bcr. In this report we demonstrate that knockdown of UAP56 blocks Bcr induced DNA synthesis in vascular smooth muscle cells (VSMC). We also found that over expression of Bcr increased the expression of cyclin E and decreased the expression of p27. Knockdown of UAP56 reversed the effect of Bcr on cyclin E and p27 expression. Furthermore, we found that Bcr binds to UAP56 and demonstrate that binding of UAP56 to Bcr is critical for Bcr induced DNA synthesis in VSMC. Our data identify UAP56 as an important binding partner of Bcr and a novel target for inhibiting vascular smooth muscle cell proliferation.

  6. PARM-1 promotes cardiomyogenic differentiation through regulating the BMP/Smad signaling pathway

    SciTech Connect (OSTI)

    Nakanishi, Naohiko; Takahashi, Tomosaburo; Ogata, Takehiro; Adachi, Atsuo; Imoto-Tsubakimoto, Hiroko; Ueyama, Tomomi; Matsubara, Hiroaki

    2012-11-30

    Highlights: Black-Right-Pointing-Pointer PARM-1 expression is induced during cardiomyogenesis. Black-Right-Pointing-Pointer PARM-1 expression precedes Nkx2.5 and Tbx5 during cardiomyogenesis. Black-Right-Pointing-Pointer PARM-1 activates BMP/Smad signaling. Black-Right-Pointing-Pointer PARM-1 enhances cardiac specification, resulting in promoted cardiomyogenesis. -- Abstract: PARM-1, prostatic androgen repressed message-1, is an endoplasmic reticulum (ER) molecule that is involved in ER stress-induced apoptosis in cardiomyocytes. In this study, we assessed whether PARM-1 plays a role in the differentiation of stem cells into cardiomyocytes. While PARM-1 was not expressed in undifferentiated P19CL6 embryonic carcinoma cells, PARM-1 expression was induced during cardiomyogenic differentiation. This expression followed expression of mesodermal markers, and preceded expression of cardiac transcription factors. PARM-1 overexpression did not alter the expression of undifferentiated markers and the proliferative property in undifferentiated P19CL6 cells. Expression of cardiac transcription factors during cardiomyogenesis was markedly enhanced by overexpression of PARM-1, while expression of mesodermal markers was not altered, suggesting that PARM-1 is involved in the differentiation from the mesodermal lineage to cardiomyocytes. Furthermore, overexpression of PARM-1 induced BMP2 mRNA expression in undifferentiated P19CL6 cells and enhanced both BMP2 and BMP4 mRNA expression in the early phase of cardiomyogenesis. PARM-1 overexpression also enhanced phosphorylation of Smads1/5/8. Thus, PARM-1 plays an important role in the cardiomyogenic differentiation of P19CL6 cells through regulating BMP/Smad signaling pathways, demonstrating a novel role of PARM-1 in the cardiomyogenic differentiation of stem cells.

  7. Staff Listing- Office of Regulation and International Engagement

    Broader source: Energy.gov [DOE]

    Mailing Address:Office of Regulation and International EngagementOffice of Fossil EnergyFE-341000 Independence Ave. S.W.Washington, DC 20585

  8. Title 22 California Code of Regulations | Open Energy Information

    Open Energy Info (EERE)

    Abstract Title 22 California Code of Regulations, current through August 7, 2014. Published NA Year Signed or Took Effect 2014 Legal Citation Title 22 California Code...

  9. Providing Minute-to-Minute Regulation from Wind Plants: Preprint

    SciTech Connect (OSTI)

    Kirby, B.; Milligan, M.; Ela, E.

    2010-10-01

    In this paper, we extend the previous analysis using time series data from existing wind plants, system loads, and regulation and energy markets.

  10. Chapter 1 of the Wyoming Public Service Commission Regulations...

    Open Energy Info (EERE)

    of the Wyoming Public Service Commission Regulations: Rules of Practice and Procedure Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  11. Rules and Regulations of the Department of Transportation, Chapter...

    Open Energy Info (EERE)

    of Transportation, Chapter 13 - Access FacilitiesLegal Abstract This chapter sets forth Department of Transportation rules and regulations governing access facilities....

  12. USACE Engineer Regulation 1110-2-1454 Corps Responsibilities...

    Open Energy Info (EERE)

    USACE Engineer Regulation 1110-2-1454 Corps Responsibilities for Non-Federal Hydroelectric Power Development under the Federal Power Act Jump to: navigation, search OpenEI...

  13. Council on Environmental Quality (CEQ) NEPA Regulations: 40 CFR...

    Open Energy Info (EERE)

    LibraryAdd to library Legal Document- Secondary Legal SourceSecondary Legal Source: Council on Environmental Quality (CEQ) NEPA Regulations: 40 CFR 1500 - 1518Legal Author CEQ...

  14. Standards for Municipal Small Wind Regulations and Model Ordinance

    Office of Energy Efficiency and Renewable Energy (EERE)

    In July 2008, New Hampshire enacted legislation designed to prevent municipalities from adopting ordinances or regulations that place unreasonable limits on or hinder the performance of wind energy...

  15. Exceptions to DOE Rules and Regulations | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Exceptions to DOE Rules and Regulations Exceptions to DOE Rules and Regulations The Department of Energy (DOE) issues test procedures and regulations covering a number of products, but does provide for exceptions to these rules under certain circumstances. Any person, as defined by 10 CFR 1003.2, can apply to the DOE for an exception from a rule, regulation, or DOE action having the effect of a rule, as provided for in section 504 of 42 U.S.C. 7194 and codified in 10 CFR 1003 subpart B. The DOE

  16. Biomass Boiler and Furnace Emissions and Safety Regulations in...

    Open Energy Info (EERE)

    in the Northeast States Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Biomass Boiler and Furnace Emissions and Safety Regulations in the Northeast States Agency...

  17. Update on Diesel Exhaust Emission Control Technology and Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Control Technology and Regulations Update on Diesel Exhaust Emission Control Technology ... Light Duty Diesels in the United States - Some Perspectives Review of Diesel Emission ...

  18. A Regulator's Perspective on Interpretation of Performance and...

    Office of Environmental Management (EM)

    Interpretation of Performance and Risk Assessment Results A Regulator's Perspective on Interpretation of Performance and Risk Assessment Results Presentation from the 2015 Annual...

  19. EO 13211: Regulations That Significantly Affect Energy Supply...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Executive Order 13211 of May 18, 2001 Actions Concerning Regulations That Significantly ... present the related submission under Executive Order 12866 of September 30, 1993, or ...

  20. Applicable EPA Regulations and Description Website | Open Energy...

    Open Energy Info (EERE)

    provides information regarding EPA regulations that apply to brownfields grants. Author Environmental Protection Agency Published EPA, Date Not Provided DOI Not Provided Check...

  1. Effective Regulatory Institutions: The Regulator's Role in the...

    Open Energy Info (EERE)

    Regulatory Institutions: The Regulator's Role in the Policy Process, Including Issues of Regulatory Independence Jump to: navigation, search Tool Summary LAUNCH TOOL Name:...

  2. Strategic Energy Management for Regulators of Ratepayer-Funded Programs

    SciTech Connect (OSTI)

    Existing Commercial Buildings Working Group

    2012-05-23

    Provides regulators with information on how to design and implement whole-building, continuous-improvement energy management programs for commercial buildings.

  3. Activation of 200 MW refusegenerated CHP upward regulation effect...

    Open Energy Info (EERE)

    regulation effect Country Denmark Headquarters Location Thisted, Denmark Coordinates 56.959167, 8.703492 Loading map... "minzoom":false,"mappingservice":"googlemaps3","typ...

  4. ADR Provisions in Federal Acquisition Regulation (FAR) | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulation (FAR) More Documents & Publications Microsoft Word - ADR-Provisions-FederalAcquisitionReg.doc ACQUISITION LETTER DEAR Part 933 Microsoft Word - ACQUISITION LETTER.doc...

  5. Policy Flash 2013-23 Department of Energy Acquisition Regulation...

    Broader source: Energy.gov (indexed) [DOE]

    Attached is Policy Flash 2013-23 Department of Energy Acquisition Regulation (DEAR) Final Rule for changes to Parts 908, 945, 952, and 970 regarding Government Property. Questions...

  6. Distribution System Voltage Regulation by Distributed Energy Resources

    SciTech Connect (OSTI)

    Ceylan, Oguzhan; Liu, Guodong; Xu, Yan; Tomsovic, Kevin

    2014-01-01

    This paper proposes a control method to regulate voltages in 3 phase unbalanced electrical distribution systems. A constrained optimization problem to minimize voltage deviations and maximize distributed energy resource (DER) active power output is solved by harmony search algorithm. IEEE 13 Bus Distribution Test System was modified to test three different cases: a) only voltage regulator controlled system b) only DER controlled system and c) both voltage regulator and DER controlled system. The simulation results show that systems with both voltage regulators and DER control provide better voltage profile.

  7. Control and regulation of modern distribution system, ForskEL...

    Open Energy Info (EERE)

    system, ForskEL (Smart Grid Project) Jump to: navigation, search Project Name Control and regulation of modern distribution system, ForskEL Country Denmark Coordinates...

  8. Acquisition Guide Chapter 1.0- Acquisition Regulations System

    Broader source: Energy.gov [DOE]

    This section discusses the Civilian Agency Acquisition Council, agency acquisition regulations, deviations from the FAR and DEAR, ratification of unauthorized commitments, and Contracting Officers and their representatives.

  9. Re: Request for Information on Regulatory Burden of DOE Regulations...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PDF icon Re: Request for Information on Regulatory Burden of DOE Regulations More Documents & Publications Edison Electric Institute (EEI) Reducing Regulatory Burden RFI, 77 Fed. ...

  10. Geothermal Regulations in Colorado - Land Ownership is the Key...

    Open Energy Info (EERE)

    Jump to: navigation, search OpenEI Reference LibraryAdd to library Conference Paper: Geothermal Regulations in Colorado - Land Ownership is the Key Abstract Geothermal resources in...

  11. TEEIC Laws and Regulations Applicable to Energy Transmission...

    Open Energy Info (EERE)

    TEEIC Laws and Regulations Applicable to Energy Transmission Development Website Jump to: navigation, search OpenEI Reference LibraryAdd to library Web Site: TEEIC Laws and...

  12. 50 CFR 216 - Regulations Governing the Taking and Importing of...

    Open Energy Info (EERE)

    50 CFR 216 - Regulations Governing the Taking and Importing of Marine Mammals Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  13. A Utility Regulator's Guide to Data Access for Commercial Building...

    Broader source: Energy.gov (indexed) [DOE]

    use data to help commercial customers manage energy costs through building energy benchmarking. A Utility Regulator's Guide to Data Access for Commercial Building Energy...

  14. DOE's Program Regulating Liquefied Natural Gas Export Applications...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE's Program Regulating Liquefied Natural Gas Export Applications June 18, 2013 - 10:15am Addthis Statement of Christopher Smith, Acting Assistant Secretary for Fossil Energy ...

  15. 10 V.S.A. Chapter 41 Regulation of Stream Flow | Open Energy...

    Open Energy Info (EERE)

    1 Regulation of Stream Flow Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 10 V.S.A. Chapter 41 Regulation of Stream...

  16. Title 10 Chapter 41 Regulation of Stream Flow | Open Energy Informatio...

    Open Energy Info (EERE)

    41 Regulation of Stream Flow Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Title 10 Chapter 41 Regulation of Stream...

  17. 5 CCR 1002-82 401 Certification Regulation | Open Energy Information

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 5 CCR 1002-82 401 Certification RegulationLegal Abstract Implementing regulations that...

  18. Emerging Latin American air quality regulation

    SciTech Connect (OSTI)

    Hosmer, A.W.; Vitale, E.M.; Guerrero, C.R.; Solorzano-Vincent, L.

    1998-12-31

    Latin America is the most urbanized region in the developing world. In recent years, significant economic growth has resulted in population migration from rural areas to urban centers, as well as in a substantial rise in the standard of living within the Region. These changes have impacted the air quality of Latin American countries as increased numbers of industrial facilities and motor vehicles release pollutants into the air. With the advent of new free trade agreements such as MERCOSUR and NAFTA, economic activity and associated pollutant levels can only be expected to continue to expand in the future. In order to address growing air pollution problems, many Latin America countries including Argentina, Brazil, Chile, Columbia, Costa Rica, and Mexico have passed, or will soon pass, new legislation to develop and strengthen their environmental frameworks with respect to air quality. As a first step toward understanding the impacts that this increased environmental regulation will have, this paper will examine the regulatory systems in six Latin American countries with respect to ambient air quality and for each of these countries: review a short history of the air quality problems within the country; outline the legal and institutional framework including key laws and implementing institutions; summarize in brief the current status of the country in terms of program development and implementation; and identify projected future trends. In addition, the paper will briefly review the international treaties that have bearing on Latin American air quality. Finally, the paper will conclude by identifying and exploring emerging trends in individual countries and the region as a whole.

  19. Regulation of the P2X7R by microRNA-216b in human breast cancer

    SciTech Connect (OSTI)

    Zheng, Luming; Zhang, Xukui; Yang, Feng; Zhu, Jian; Zhou, Peng; Yu, Fang; Hou, Lei; Xiao, Lei; He, Qingqing; Wang, Baocheng

    2014-09-12

    Highlights: • We suggest the expression level of miR-216b and P2X7R in breast cancer tissues and cell lines. • We demonstrated that miR-216b directly targets and inhibits P2X7R. • We suggested miR-216b can attenuate ATP/P2X7R signaling pathways and induced Bcl-2/caspase-3 pathway. - Abstract: Breast cancer is the most common cancer in women around the world. However, the molecular mechanisms underlying breast cancer pathogenesis are only partially understood. Here, in this study, we found that P2X7R was up-regulated and miR-216b was down-regulated in breast cancer cell lines and tissues. Using bioinformatic analysis and 3′UTR luciferase reporter assay, we determined P2X7R can be directly targeted by miR-216b, which can down-regulate endogenous P2X7R mRNA and protein levels. Ectopic expression of miR-216b mimics leads to inhibited cell growth and apoptosis, while blocking expression of the miR-216b results in increased cell proliferation. Furthermore, our findings demonstrate that knockdown of P2X7R promotes apoptosis in breast cancer cells through down-regulating Bcl-2 and increasing the cleavage caspase-3 protein level. Finally, we confirmed that down-regulation of miR-216b in breast cancer is inversely associated with P2X7R expression level. Together, these findings establish miR-216b as a novel regulator of P2X7R and a potential therapeutic target for breast cancer.

  20. Tunicamycin-induced unfolded protein response in the developing mouse brain

    SciTech Connect (OSTI)

    Wang, Haiping; Wang, Xin; Ke, Zun-Ji; Comer, Ashley L.; Xu, Mei; Frank, Jacqueline A.; Zhang, Zhuo; Shi, Xianglin; Luo, Jia

    2015-03-15

    Accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) causes ER stress, resulting in the activation of the unfolded protein response (UPR). ER stress and UPR are associated with many neurodevelopmental and neurodegenerative disorders. The developing brain is particularly susceptible to environmental insults which may cause ER stress. We evaluated the UPR in the brain of postnatal mice. Tunicamycin, a commonly used ER stress inducer, was administered subcutaneously to mice of postnatal days (PDs) 4, 12 and 25. Tunicamycin caused UPR in the cerebral cortex, hippocampus and cerebellum of mice of PD4 and PD12, which was evident by the upregulation of ATF6, XBP1s, p-eIF2α, GRP78, GRP94 and MANF, but failed to induce UPR in the brain of PD25 mice. Tunicamycin-induced UPR in the liver was observed at all stages. In PD4 mice, tunicamycin-induced caspase-3 activation was observed in layer II of the parietal and optical cortex, CA1–CA3 and the subiculum of the hippocampus, the cerebellar external germinal layer and the superior/inferior colliculus. Tunicamycin-induced caspase-3 activation was also shown on PD12 but to a much lesser degree and mainly located in the dentate gyrus of the hippocampus, deep cerebellar nuclei and pons. Tunicamycin did not activate caspase-3 in the brain of PD25 mice and the liver of all stages. Similarly, immature cerebellar neurons were sensitive to tunicamycin-induced cell death in culture, but became resistant as they matured in vitro. These results suggest that the UPR is developmentally regulated and the immature brain is more susceptible to ER stress. - Highlights: • Tunicamycin caused a development-dependent UPR in the mouse brain. • Immature brain was more susceptible to tunicamycin-induced endoplasmic reticulum stress. • Tunicamycin caused more neuronal death in immature brain than mature brain. • Tunicamycin-induced neuronal death is region-specific.

  1. Federal Regulations for Natural Gas Imports and Exports | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Federal Regulations for Natural Gas Imports and Exports Federal Regulations for Natural Gas Imports and Exports Section 3 of the Natural Gas Act (61.35 KB) More Documents & Publications Appendix B Patent and copyright cases 42 USC 17013

  2. Update on Diesel Exhaust Emission Control Technology and Regulations |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Control Technology and Regulations Update on Diesel Exhaust Emission Control Technology and Regulations 2004 Diesel Engine Emissions Reduction (DEER) Conference Presentation: Corning 2004_deer_johnson2.pdf (1.48 MB) More Documents & Publications Light Duty Diesels in the United States - Some Perspectives Review of Diesel Emission Control Technology Update on Diesel Exhaust Emission Control

  3. Protocadherin-7 induces bone metastasis of breast cancer

    SciTech Connect (OSTI)

    Li, Ai-Min; Tian, Ai-Xian; Zhang, Rui-Xue; Ge, Jie; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin ; Sun, Xuan; Cao, Xu-Chen; Key Laboratory of Breast Cancer Prevention and Treatment of the Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin

    2013-07-05

    Highlights: PCDH7 is overexpression in high bone metastatic MDA-MB-231 cells. PCDH7 is up-regulation in bone metastatic breast cancer tissues. Suppression of PCDH7 inhibits cell proliferation, migration, and invasion in vitro. PCDH7 induces breast cancer bone metastasis in vivo. -- Abstract: Breast cancer had a propensity to metastasize to bone, resulting in serious skeletal complications associated with poor outcome. Previous study showed that Protocadherin-7 (PCDH7) play an important role in brain metastatic breast cancer, however, the role of PCDH7 in bone metastatic breast cancer has never been explored. In the present study, we found that PCDH7 expression was up-regulation in bone metastatic breast cancer tissues by real-time PCR and immunohistochemistry assays. Furthermore, suppression of PCDH7 inhibits breast cancer cell proliferation, migration, and invasion in vitro by MTT, scratch, and transwell assays. Most importantly, overexpression of PCDH7 promotes breast cancer cell proliferation and invasion in vitro, and formation of bone metastasis in vivo. These data provide an important insight into the role of PCDH7 in bone metastasis of breast cancer.

  4. Voltage Regulator Chip: Power Supplies on a Chip

    SciTech Connect (OSTI)

    2010-09-01

    ADEPT Project: CPES at Virginia Tech is finding ways to save real estate on a computer's motherboard that could be used for other critical functions. Every computer processor today contains a voltage regulator that automatically maintains a constant level of electricity entering the device. These regulators contain bulky components and take up about 30% of a computer's motherboard. CPES at Virginia Tech is developing a voltage regulator that uses semiconductors made of gallium nitride on silicon (GaN-on-Si) and high-frequency soft magnetic material. These materials are integrated on a small, 3D chip that can handle the same amount of power as traditional voltage regulators at 1/10 the size and with improved efficiency. The small size also frees up to 90% of the motherboard space occupied by current voltage regulators.

  5. Immunities from the antitrust laws for regulated entities

    SciTech Connect (OSTI)

    Jones, M.B.

    1985-05-30

    Just as regulation is an ever-present and crucial factor in most of the day-to-day operating decisions of regulated natural gas transmission and distribution companies, it is also an important factor to consider in assessing the application of the antitrust laws to the conduct of these regulated entities. The legal guidelines are far from clear and are evolving. However, as regulated entities find themselves in an ever more competitive environment, it becomes necessary for their legal counsel to monitor closely the developments in the law concerning immunities from the antitrust laws by virtue of regulation imposed by government. The author hopes that this article will provide a useful starting point for that analysis.

  6. Sox5 induces epithelial to mesenchymal transition by transactivation of Twist1

    SciTech Connect (OSTI)

    Pei, Xin-Hong; Lv, Xin-Quan; Li, Hui-Xiang

    2014-03-28

    Highlights: • Depletion of Sox5 inhibits breast cancer proliferation, migration, and invasion. • Sox5 transactivates Twist1 expression. • Sox5 induces epithelial to mesenchymal transition through transactivation of Twist1 expression. - Abstract: The epithelial to mesenchymal transition (EMT), a highly conserved cellular program, plays an important role in normal embryogenesis and cancer metastasis. Twist1, a master regulator of embryonic morphogenesis, is overexpressed in breast cancer and contributes to metastasis by promoting EMT. In exploring the mechanism underlying the increased Twist1 in breast cancer cells, we found that the transcription factor SRY (sex-determining region Y)-box 5(Sox5) is up-regulation in breast cancer cells and depletion of Sox5 inhibits breast cancer cell proliferation, migration, and invasion. Furthermore, depletion of Sox5 in breast cancer cells caused a dramatic decrease in Twist1 and chromosome immunoprecipitation assay showed that Sox5 can bind directly to the Twist1 promoter, suggesting that Sox5 transactivates Twist1 expression. We further demonstrated that knockdown of Sox5 up-regulated epithelial phenotype cell biomarker (E-cadherin) and down-regulated mesenchymal phenotype cell biomarkers (N-cadherin, Vimentin, and Fibronectin 1), resulting in suppression of EMT. Our study suggests that Sox5 transactivates Twist1 expression and plays an important role in the regulation of breast cancer progression.

  7. SIRT1 inactivation induces inflammation through the dysregulation of autophagy in human THP-1 cells

    SciTech Connect (OSTI)

    Takeda-Watanabe, Ai; Kitada, Munehiro; Kanasaki, Keizo; Koya, Daisuke

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer SIRT1 inactivation decreases autophagy in THP-1 cell. Black-Right-Pointing-Pointer Inhibition of autophagy induces inflammation. Black-Right-Pointing-Pointer SIRT1 inactivation induces inflammation through NF-{kappa}B activation. Black-Right-Pointing-Pointer The p62/Sqstm1 accumulation by impairment of autophagy is related to NF-{kappa}B activation. Black-Right-Pointing-Pointer SIRT1 inactivation is involved in the activation of mTOR and decreased AMPK activation. -- Abstract: Inflammation plays a crucial role in atherosclerosis. Monocytes/macrophages are some of the cells involved in the inflammatory process in atherogenesis. Autophagy exerts a protective effect against cellular stresses like inflammation, and it is regulated by nutrient-sensing pathways. The nutrient-sensing pathway includes SIRT1, a NAD{sup +}-dependent histone deacetylase, which is implicated in the regulation of a variety of cellular processes including inflammation and autophagy. The mechanism through which the dysfunction of SIRT1 contributes to the regulation of inflammation in relation to autophagy in monocytes/macrophages is unclear. In the present study, we demonstrate that treatment with 2-[(2-Hydroxynaphthalen-1-ylmethylene)amino]-N-(1-phenethyl)benzamide (Sirtinol), a chemical inhibitor of SIRT1, induces the overexpression of inflammation-related genes such as tumor necrosis factor (TNF)-{alpha} and interleukin (IL)-6 through nuclear factor (NF)-{kappa}B signaling activation, which is associated with autophagy dysfunction, as shown through p62/Sqstm1 accumulation and decreased expression of light chain (LC) 3 II in THP-1 cells. The autophagy inhibitor, 3-methyladenine, also induces inflammation-related NF-{kappa}B activation. In p62/Sqstm1 knockdown cells, Sirtinol-induced inflammation through NF-{kappa}B activation is blocked. In addition, inhibition of SIRT1 is involved in the activation of the mammalian target of rapamycin (mTOR) pathway and

  8. Ion-induced nuclear radiotherapy

    DOE Patents [OSTI]

    Horn, K.M.; Doyle, B.L.

    1996-08-20

    Ion-induced Nuclear Radiotherapy (INRT) is a technique for conducting radiosurgery and radiotherapy with a very high degree of control over the spatial extent of the irradiated volume and the delivered dose. Based upon the concept that low energy, ion induced atomic and nuclear reactions can be used to produce highly energetic reaction products at the site of a tumor, the INRT technique is implemented through the use of a conduit-needle or tube which conducts a low energy ion beam to a position above or within the intended treatment area. At the end of the conduit-needle or tube is a specially fabricated target which, only when struck by the ion beam, acts as a source of energetic radiation products. The inherent limitations in the energy, and therefore range, of the resulting reaction products limits the spatial extent of irradiation to a pre-defined volume about the point of reaction. Furthermore, since no damage is done to tissue outside this irradiated volume, the delivered dose may be made arbitrarily large. INRT may be used both as a point-source of radiation at the site of a small tumor, or as a topical bath of radiation to broad areas of diseased tissue. 25 figs.

  9. Ion-induced nuclear radiotherapy

    DOE Patents [OSTI]

    Horn, Kevin M.; Doyle, Barney L.

    1996-01-01

    Ion-induced Nuclear Radiotherapy (INRT) is a technique for conducting radiosurgery and radiotherapy with a very high degree of control over the spatial extent of the irradiated volume and the delivered dose. Based upon the concept that low energy, ion induced atomic and nuclear reactions can be used to produce highly energetic reaction products at the site of a tumor, the INRT technique is implemented through the use of a conduit-needle or tube which conducts a low energy ion beam to a position above or within the intended treatment area. At the end of the conduit-needle or tube is a specially fabricated target which, only when struck by the ion beam, acts as a source of energetic radiation products. The inherent limitations in the energy, and therefore range, of the resulting reaction products limits the spatial extent of irradiation to a pre-defined volume about the point of reaction. Furthermore, since no damage is done to tissue outside this irradiated volume, the delivered dose may be made arbitrarily large. INRT may be used both as a point-source of radiation at the site of a small tumor, or as a topical bath of radiation to broad areas of diseased tissue.

  10. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  11. A regulator`s perspective on NRC`s participation in the operations & maintenance committees

    SciTech Connect (OSTI)

    Wessman, R.H.

    1996-12-01

    As a regulator fairly new to the American Society of Mechanical Engineers (ASME) Operations and Maintenance (O&M) Committee process, the author does not have a personal historical perspective as do many of the longer-term, and highly respected, members of the O&M Committee. However, as Branch Chief of the Mechanical Engineering Branch, Division of Engineering, in the Office of Nuclear Reactor Regulation at the NRC for just over two years, he has responsibility for the regulatory agency`s review of licensee actions involving the products that come from the efforts of the O&M Committee, as well as responsibility for portions of the activities of interest to other ASME Code groups such as Section III, Section XI, and Qualification of Mechanical Equipment. As a result, the author has learned a great deal about the code process in a short time. Here he gives his perspectives on the process and provides a few thoughts on the direction for the future.

  12. A Test Bed for Self-regulating Distribution Systems: Modeling Intergrated Renewable Energy and Demand Response in the GridLAB-D/MATLAB Environment

    SciTech Connect (OSTI)

    Wang, Dan; de Wit, Braydon; Parkinson, Simon; Fuller, Jason C.; Chassin, David P.; Crawford, Curran; Djilali, Ned

    2012-01-16

    This paper discusses the development of a simulation test bed permitting the study of integrated renewable energy generators and controlled distributed heat pumps operating within distribution systems. The test bed is demonstrated in this paper by addressing the important issue of the self-regulating effect of consumer-owned air-source heat pumps on the variability induced by wind power integration, particularly when coupled with increased access to demand response realized through a centralized load control strategy.

  13. Regulation of MDA-MB-231 cell proliferation by GSK-3? involves epigenetic modifications under high glucose conditions

    SciTech Connect (OSTI)

    Gupta, Chanchal; Kaur, Jasmine; Tikoo, Kulbhushan

    2014-05-15

    Hyperglycemia is a critical risk factor for development and progression of breast cancer. We have recently reported that high glucose induces phosphorylation of histone H3 at Ser 10 as well as de-phosphorylation of GSK-3? at Ser 9 in MDA-MB-231 cells. Here, we elucidate the mechanism underlying hyperglycemia-induced proliferation in MDA-MB-231 breast cancer cells. We provide evidence that hyperglycemia led to increased DNA methylation and DNMT1 expression in MDA-MB-231 cells. High glucose condition led to significant increase in the expression of PCNA, cyclin D1 and decrease in the expression of PTPN 12, p21 and PTEN. It also induced hypermethylation of DNA at the promoter region of PTPN 12, whereas hypomethylation at Vimentin and Snail. Silencing of GSK-3? by siRNA prevented histone H3 phosphorylation and reduced DNMT1 expression. We show that chromatin obtained after immunoprecipitation with phospho-histone H3 was hypermethylated under high glucose condition, which indicates a cross-talk between DNA methylation and histone H3 phosphorylation. ChIP-qPCR analysis revealed up-regulation of DNMT1 and metastatic genes viz. Vimentin, Snail and MMP-7 by phospho-histone H3, which were down-regulated upon GSK-3? silencing. To the best of our knowledge, this is the first report which shows that interplay between GSK-3? activation, histone H3 phosphorylation and DNA methylation directs proliferation of breast cancer cells. - Highlights: High glucose induces phosphorylation of histone H3 and dephosphorylation of GSK-3?. Moreover, hyperglycemia also leads to increased DNA methylation in MDA-MB-231 cells. Inhibition of GSK-3? prevented histone H3 phosphorylation and reduced DNMT1 levels. Interplay exists between GSK-3?, histone H3 phosphorylation and DNA methylation.

  14. Extracellular Matrix-Regulated Gene Expression RequiresCooperation of SWI/SNF and Transcription Factors

    SciTech Connect (OSTI)

    Xu, Ren; Spencer, Virginia A.; Bissell, Mina J.

    2006-05-25

    Extracellular cues play crucial roles in the transcriptional regulation of tissue-specific genes, but whether and how these signals lead to chromatin remodeling is not understood and subject to debate. Using chromatin immunoprecipitation (ChIP) assays and mammary-specific genes as models, we show here that extracellular matrix (ECM) molecules and prolactin cooperate to induce histone acetylation and binding of transcription factors and the SWI/SNF complex to the {beta}- and ?-casein promoters. Introduction of a dominant negative Brg1, an ATPase subunit of SWI/SNF complex, significantly reduced both {beta}- and ?-casein expression, suggesting that SWI/SNF-dependent chromatin remodeling is required for transcription of mammary-specific genes. ChIP analyses demonstrated that the ATPase activity of SWI/SNF is necessary for recruitment of RNA transcriptional machinery, but not for binding of transcription factors or for histone acetylation. Coimmunoprecipitation analyses showed that the SWI/SNF complex is associated with STAT5, C/EBP{beta}, and glucocorticoid receptor (GR). Thus, ECM- and prolactin-regulated transcription of the mammary-specific casein genes requires the concerted action of chromatin remodeling enzymes and transcription factors.

  15. Regulation of the human interleukin-2/interleukin-2 receptor system: A role for immunosuppression

    SciTech Connect (OSTI)

    Kaempfer, R.

    1994-12-31

    The strength of the cellular immune response is regulated to a large extent by the amount of interleukin-2 (IL-2) produced in response to a stimulus. The ability of lymphocytes and other cells to respond to IL-2 depends upon the expression of cell surface IL-2 receptors. Formation of a high-affinity IL-2 receptor is regulated primarily through induction of its {alpha} subunit, IL-2R{alpha}. Once formed, the IL-2R{alpha} chain turns over rapidly, rendering expression of high-affinity IL-2 receptors during the immune response dependent upon continuous activity of the IL-2R{alpha} gene. The induced expression of both human IL-2 and IL-2R{alpha} chains is sensitive to cell-mediated suppression by CD8 cells; depletion of CD8 cells leads to extensive superinduction. This coupled suppression of IL-2 and IL-2R{alpha} genes greatly increases the extend of control, and strongly limits the strength, of the signal transduced by this ligand/receptor system during an immune response. 29 refs., 3 figs.

  16. Identification of GPR65, a novel regulator of matrix metalloproteinases using high through-put screening

    SciTech Connect (OSTI)

    Xu, Hongbo; Chen, Xiaohong; Huang, Junwei [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Deng, Weiwei [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China)] [Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Zhong, Qi [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China); Yue, Changli [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China)] [Department of Pathology, Beijing Tongren Hospital, Capital Medical University, Beijing (China); Wang, Pingzhang, E-mail: wangpzh@bjmu.edu.cn [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China) [Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Peking University Center for Human Disease Genomics, Key Laboratory of Medical Immunology, Ministry of Health (China); Functional Genomics Group, Chinese National Human Genome Center (CHGB) at Beijing (China); Huang, Zhigang, E-mail: enthuangzhigang@sohu.com [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)] [Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Key Laboratory of Otolaryngology Head and Neck Surgery, Beijing (China)

    2013-06-21

    Highlights: A novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors was defined. GPR65 was identified to induce the MMP3 expression. GPR65 mediated MMP induction under acidic conditions. AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. GPR65 overexpression can accelerate the invision of A549 cells. -- Abstract: Matrix metalloproteinases (MMPs) are over-expressed in nearly all cancers. To study novel regulatory factors of MMP expression in head and neck cancer (HNC), we screened a total of 636 candidate genes encoding putative human transmembrane proteins using MMP promoter reporter in a dual luciferase assay system. Three genes GPR65, AXL and TNFRSF10B dramatically activated the induction of MMP3 expression. The induction of MMP expression by GPR65 was further confirmed in A549 and/or FaDu cells. GPR65 mediated MMP induction under acidic conditions. The AP-1 binding site in MMP3 promoter was crucial for MMP3 induction. Moreover, the A549 cells infected by recombinant adenovirus of GPR65 showed accelerated cell invasion. In conclusion, we validate that GPR65 is vital regulatory genes upstream of MMP3, and define a novel mechanism of MMP3 regulation by proton-sensing G-protein-coupled receptors.

  17. Inhibitory effect of cucurbitacin B on imiquimod-induced skin inflammation

    SciTech Connect (OSTI)

    Li, Zheng Jun; Shin, Jung-Min; Choi, Dae-Kyoung; Lim, Seul Ki; Yoon, Tae-Jin; Lee, Young Ho; Sohn, Kyung-Cheol; Im, Myung; Lee, Young; Seo, Young-Joon; Kim, Chang Deok; Lee, Jeung-Hoon

    2015-04-17

    Psoriasis is a common skin disease, of which pathogenesis involves the increase of inflammatory reaction in epidermal cells. In an attempt to find therapeutics for psoriasis, we found that cucurbitacin B has an inhibitory potential on imiquimod-induced inflammation of keratinocytes. Cucurbitacin B significantly inhibited imiquimod-induced expression of crucial psoriatic cytokines, such as IL-8 and CCL20, via down-regulation of NF-κB and STAT3 signaling pathway in human keratinocytes. In addition, keratinocyte proliferation was markedly inhibited by cucurbitacin B. The potential beneficial effect of cucurbitacin B on psoriasis was further validated in imiquimod-induced psoriasiform dermatitis of experimental animal. Topical application of cucurbitacin B resulted in significant reduction of epidermal hyperplasia and inflammatory cytokines production, and ameliorated the psoriatic symptom. Taken together, these results suggest that cucurbitacin B may be a potential candidate for the treatment of psoriasis. - Highlights: • Cucurbitacin B has a potential for inhibiting the growth of keratinocytes. • Cucurbitacin B inhibits imiquimod-induced inflammatory reaction in keratinocytes. • Cucurbitacin B inhibits imiquimod-induced psoriasiform dermatitis in experimental animal.

  18. TRIF promotes angiotensin II-induced cross-talk between fibroblasts and macrophages in atrial fibrosis

    SciTech Connect (OSTI)

    Chen, Xiao-Qing; Zhang, Dao-Liang; Zhang, Ming-Jian; Guo, Meng; Zhan, Yang-Yang; Liu, Fang; Jiang, Wei-Feng; Zhou, Li; Zhao, Liang; Wang, Quan-Xing; Liu, Xu

    2015-08-14

    Aims: Atrial fibroblasts and macrophages have long been thought to participate in atrial fibrillation (AF). However, which specific mediator may regulate the interaction between them remains unclear. Methods and results: We provided the evidence for the involvement of Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF), an important inflammation-related molecule, in the pathophysiology of AF. Patients with AF showed higher levels of angiotensin II (AngII) and TRIF expression and larger number of macrophages infiltration in left atria appendage than individuals with sinus rhythm (SR). In the cell study, AngII induced chemokines expressions in mouse atrial fibroblasts and AngII-stimulated atrial fibroblasts induced the chemotaxis of macrophages, which were reduced by losartan and TRIF siRNA. Meanwhile, AngII-stimulated atrial fibroblasts proliferation was enhanced by macrophages. Conclusions: Our data demonstrated that TRIF may be a crucial factor promoting the interaction between atrial fibroblasts and macrophages, leading to atrial fibrosis. - Highlights: • Compared with SR, AF showed higher TRIF expression in left atrial appendage. • TRIF siRNA reversed macrophage chemotaxis induced by AngII-treated fibroblast. • TRIF siRNA reversed chemokines expressions induced by AngII in fibroblast. • AngII-stimulated atrial fibroblast proliferation was enhanced by macrophage.

  19. SENP1 inhibition induces apoptosis and growth arrest of multiple myeloma cells through modulation of NF-κB signaling

    SciTech Connect (OSTI)

    Xu, Jun; Sun, Hui-Yan; Xiao, Feng-Jun; Wang, Hua; Yang, Yang; Wang, Lu; Gao, Chun-Ji; Guo, Zi-Kuan; Wu, Chu-Tse; Wang, Li-Sheng

    2015-05-01

    SUMO/sentrin specific protease 1 (Senp1) is an important regulation protease in the protein sumoylation, which affects the cell cycle, proliferation and differentiation. The role of Senp1 mediated protein desumoylation in pathophysiological progression of multiple myeloma is unknown. In this study, we demonstrated that Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. Lentivirus-mediated Senp1 knockdown triggers apoptosis and reduces viability, proliferation and colony forming ability of MM cells. The NF-κB family members including P65 and inhibitor protein IkBα play important roles in regulation of MM cell survival and proliferation. We further demonstrated that Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation, leading to inactivation of NF-kB signaling in MM cells. These results delineate a key role for Senp1in IL-6 induced proliferation and survival of MM cells, suggesting it may be a potential new therapeutic target in MM. - Highlights: • Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. • Senp1 knockdown triggers apoptosis and reduces proliferation of MM cells. • Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation.

  20. Identification of a chemical inhibitor for nuclear speckle formation: Implications for the function of nuclear speckles in regulation of alternative pre-mRNA splicing

    SciTech Connect (OSTI)

    Kurogi, Yutaro; Matsuo, Yota; Mihara, Yuki; Yagi, Hiroaki; Shigaki-Miyamoto, Kaya; Toyota, Syukichi; Azuma, Yuko; Igarashi, Masayuki; Tani, Tokio

    2014-03-28

    Highlights: • We identified tubercidin as a compound inducing aberrant formation of the speckles. • Tubercidin causes delocalization of poly (A){sup +}RNAs from nuclear speckles. • Tubercidin induces dispersion of splicing factors from nuclear speckles. • Tubercidin affects alternative pre-mRNA splicing. • Nuclear speckles play a role in regulation of alternative pre-mRNA splicing. - Abstract: Nuclear speckles are subnuclear structures enriched with RNA processing factors and poly (A){sup +} RNAs comprising mRNAs and poly (A){sup +} non-coding RNAs (ncRNAs). Nuclear speckles are thought to be involved in post-transcriptional regulation of gene expression, such as pre-mRNA splicing. By screening 3585 culture extracts of actinomycetes with in situ hybridization using an oligo dT probe, we identified tubercidin, an analogue of adenosine, as an inhibitor of speckle formation, which induces the delocalization of poly (A){sup +} RNA and dispersion of splicing factor SRSF1/SF2 from nuclear speckles in HeLa cells. Treatment with tubercidin also decreased steady-state MALAT1 long ncRNA, thought to be involved in the retention of SRSF1/SF2 in nuclear speckles. In addition, we found that tubercidin treatment promoted exon skipping in the alternative splicing of Clk1 pre-mRNA. These results suggest that nuclear speckles play a role in modulating the concentration of splicing factors in the nucleoplasm to regulate alternative pre-mRNA splicing.

  1. C-MYC REGULATES SUBSTRATE OXIDATION PATTERNS DURING EARLY PRESSURE-OVERLOAD HYPERTROPHY

    SciTech Connect (OSTI)

    Ledee, Dolena R.; Smith, Lincoln; Kajimoto, Masaki; Bruce, Margaret; Isern, Nancy G.; Xu, Chun; Portman, Michael A.; Olson, Aaron

    2013-11-26

    Pressure overload cardiac hypertrophy alters substrate metabolism. Prior work showed that myocardial inactivation of c-Myc (Myc) attenuated hypertrophy and decreased expression of glycolytic genes after aortic constriction. Accordingly, we hypothesize that Myc regulates substrate preferences for the citric acid cycle during pressure overload hypertrophy from transverse aortic constriction (TAC) and that these metabolic changes impact cardiac function and growth. To test this hypothesis, we subjected FVB mice with cardiac specific, inducible Myc inactivation (MycKO-TAC) and non-transgenic littermates (Cont-TAC) to transverse aortic constriction (n=7/group). A separate group underwent sham surgery (Sham, n=5). After two weeks, function was measured in isolated working hearts along with substrate fractional contributions to the citric acid cycle by using perfusate with 13C labeled mixed fatty acids, lactate, ketones and unlabeled glucose and insulin. Western blots were used to evaluate metabolic enzymes. Cardiac function was similar between groups after TAC although +dP/dT and -dP/dT trended towards improvement in MycKO-TAC versus Cont-TAC. Compared to Sham, Cont-TAC had increased free fatty acid fractional contribution with a concurrent decrease in unlabeled (presumably glucose) contribution. Myc inactivation (MycKO-TAC) inhibited these metabolic changes. Hypertrophy in general increased protein levels of PKM2; however this change was not linked to Myc status. Protein post-translation modification by O-GlcNAc was significantly greater in Cont-TAC versus both Sham and MycKO-TAC. In conclusion, Myc regulates substrate utilization during early pressure overload hypertrophy. Our results show that the metabolic switch during hypertrophy is not necessary to maintain cardiac function, but it may be important mechanism to promote cardiomyocyte growth. Myc also regulates protein O-GlcNAcylation during hypertrophy.

  2. Experimental validation of advanced regulations for superconducting magnet cooling undergoing periodic heat loads

    SciTech Connect (OSTI)

    Lagier, B.; Rousset, B.; Hoa, C.; Bonnay, P.

    2014-01-29

    Superconducting magnets used in tokamaks undergo periodic heat load caused by cycling plasma operations inducing AC losses, neutrons fluxes and eddy currents in magnet structures. In the cryogenic system of JT60-SA tokamak, the Auxiliary Cold Box (ACB) distributes helium from the refrigerator to the cryogenic users and in particular to the superconducting magnets. ACB comprises a saturated helium bath with immersed heat exchangers, extracting heat from independent cooling loops. The supercritical helium flow in each cooling loop is driven by a cold circulator. In order to safely operate the refrigerator during plasma pulses, the interface between the ACB and the refrigerator shall be as stable as possible, with well-balanced bath inlet and outlet mass flows during cycling operation. The solution presented in this paper relies on a combination of regulations to smooth pulsed heat loads and to keep a constant refrigeration power during all the cycle. Two smoothing strategies are presented, both regulating the outlet mass flow of the bath: the first one using the bath as a thermal buffer and the second one storing energy in the loop by varying the cold circulator speed. The bath outlet mass flow is also controlled by an immersed resistive heater which enables a constant evaporation rate in the bath when power coming from the loops is decreasing. The refrigeration power is controlled so that the compensating power remains within an acceptable margin. Experimental validation is achieved using the HELIOS facility. This facility running at CEA Grenoble since 2010 is a scaled down model of the ACB bath and Central Solenoid magnet cooling loop of the JT60-SA tokamak. Test results show performances and robustness of the regulations.

  3. A primer on incentive regulation for electric utilities

    SciTech Connect (OSTI)

    Hill, L.J.

    1995-10-01

    In contemplating a regulatory approach, the challenge for regulators is to develop a model that provides incentives for utilities to engage in socially desirable behavior. In this primer, we provide guidance on this process by discussing (1) various models of economic regulation, (2) problems implementing these models, and (3) the types of incentives that various models of regulation provide electric utilities. We address five regulatory models in depth. They include cost-of-service regulation in which prudently incurred costs are reflected dollar-for-dollar in rates and four performance-based models: (1) price-cap regulation, in which ceilings are placed on the average price that a utility can charge its customers; (2) revenue-cap regulation, in which a ceiling is placed on revenues; (3) rate-of-return bandwidth regulation, in which a utility`s rates are adjusted if earnings fall outside a {open_quotes}band{close_quotes} around equity returns; and (4) targeted incentives, in which a utility is given incentives to improve specific components of its operations. The primary difference between cost-of-service and performance-based approaches is the latter sever the tie between costs and prices. A sixth, {open_quotes}mixed approach{close_quotes} combines two or more of the five basic ones. In the recent past, a common mixed approach has been to combine targeted incentives with cost-of-service regulation. A common example is utilities that are subject to cost-of-service regulation are given added incentives to increase the efficiency of troubled electric-generating units.

  4. Metformin suppresses CYP1A1 and CYP1B1 expression in breast cancer cells by down-regulating aryl hydrocarbon receptor expression

    SciTech Connect (OSTI)

    Do, Minh Truong; Kim, Hyung Gyun; Tran, Thi Thu Phuong; Khanal, Tilak; Choi, Jae Ho; Chung, Young Chul; Jeong, Tae Cheon; Jeong, Hye Gwang

    2014-10-01

    Induction of cytochrome P450 (CYP) 1A1 and CYP1B1 by environmental xenobiotic chemicals or endogenous ligands through the activation of the aryl hydrocarbon receptor (AhR) has been implicated in a variety of cellular processes related to cancer, such as transformation and tumorigenesis. Here, we investigated the effects of the anti-diabetes drug metformin on expression of CYP1A1 and CYP1B1 in breast cancer cells under constitutive and inducible conditions. Our results indicated that metformin down-regulated the expression of CYP1A1 and CYP1B1 in breast cancer cells under constitutive and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced conditions. Down-regulation of AhR expression was required for metformin-mediated decreases in CYP1A1 and CYP1B1 expression, and the metformin-mediated CYP1A1 and CYP1B1 reduction is irrelevant to estrogen receptor α (ERα) signaling. Furthermore, we found that metformin markedly down-regulated Sp1 protein levels in breast cancer cells. The use of genetic and pharmacological tools revealed that metformin-mediated down-regulation of AhR expression was mediated through the reduction of Sp1 protein. Metformin inhibited endogenous AhR ligand-induced CYP1A1 and CYP1B1 expression by suppressing tryptophan-2,3-dioxygenase (TDO) expression in MCF-7 cells. Finally, metformin inhibits TDO expression through a down-regulation of Sp1 and glucocorticoid receptor (GR) protein levels. Our findings demonstrate that metformin reduces CYP1A1 and CYP1B1 expression in breast cancer cells by down-regulating AhR signaling. Metformin would be able to act as a potential chemopreventive agent against CYP1A1 and CYP1B1-mediated carcinogenesis and development of cancer. - Graphical abstract: Schematic of the CYP1A1 and CYP1B1 gene regulation by metformin. - Highlights: • Metformin inhibits CYP1A1 and CYP1B1 expression. • Metformin down-regulates the AhR signaling. • Metformin reduces Sp1 protein expression. • Metformin suppresses TDO expression.

  5. Industry Self-Regulation as a Means to Promote Nonproliferation

    SciTech Connect (OSTI)

    Hund, Gretchen; Elkhamri, Oksana O.

    2005-10-01

    Companies within numerous industries that have been “early adopters” of self-regulation concept, considering the environment and society alongside business issues, have realized several benefits and some competitive advantage while substantially improving their environmental performance. Given that proliferation prevention is also a public good, our premise is that the experience gained and lessons learned from the self-regulation initiative in other industries and more broadly in the arena of sustainable development provide a basis for examining the feasibility of developing self-regulation mechanisms applicable to industries involved with sensitive technologies (nuclear, radiological source, and other dual-use industries)

  6. Enforcement Regulations and Directives - Worker Safety and Health |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Worker Safety and Health Enforcement Regulations and Directives - Worker Safety and Health Regulations 10 C.F.R. Part 851 - Worker Safety and Health Program; Final Rule 10 C.F.R. Part 850 - Chronic Beryllium Disease Prevention Program; Final Rule 29 C.F.R. Part 1910 - Occupational Safety and Health Standards (OSHA) 29 C.F.R. Part 1926 - Safety and Health Regulations for Construction (OSHA) 29 C.F.R. Part 1904 - Reporting and Reporting Occupational Injuries and Illnesses

  7. Environmental regulation of coal mining, SMCRA`s second decade

    SciTech Connect (OSTI)

    McElfish, J.M.; Beier, A.E.

    1990-12-31

    This controversial book examines environmental regulation of coal mining in the United States over the 1979-1989 period. The Surface Mining Control and Reclamation Act of 1977 (SMCRA) established federal regulation of the environmental effects of coal mining. The permanent regulatory program implementing SMCRA was initiated in 1979. This book looks at the first ten years of SMCRA, seeking to draw lessons from experience that will improve regulation, so that the objectives of the law may be achieved in SMCRA`s second decade.

  8. Regulating for the long term: SMCRA and acid mine drainage

    SciTech Connect (OSTI)

    Shea, C.W.

    1995-12-31

    With the passage of the Surface Mining Control and Reclamation Act of 1977 (SMCRA), regulators and industry representatives expected to solve the problem of pollution of the Nation`s waterways caused by acidic discharges from coal mines. Eighteen years after the passage of SMCRA, hard issues of predicting, regulating and treating acid mine drainage remain. Acid mine drainage is most common in the coal seams of the Midwest and Appalachia: Pennsylvania, West Virginia, Maryland, Ohio, Illinois, and Tennessee. This article discusses regulation of coal mines and acid mine drainage for the long term.

  9. State and federal regulation of OTEC plants in Hawaii

    SciTech Connect (OSTI)

    Keith, K.M.

    1980-09-01

    The advantages of ocean thermal energy conversion (OTEC) for Hawaii, its institutional support, projected contributions of OTEC in the future, and environmental concerns are discussed. Three experimental OTEC facilities in Hawaii are described, and the many regulations that must be observed and permits needed are described. Applicability of existing federal laws in regulating commercial-scale OTEC plants is examined, and applicable Coast Guard regulations and maritime laws are discussed briefly. Questions of state-federal relations, particularly regarding Hawaii's archipelagic claims and coastal zone, are addressed. (LEW)

  10. Chapter 1 - Acquisition Regulations System | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 - Acquisition Regulations System Chapter 1 - Acquisition Regulations System 1.1_Acquisition_Regulation_System_0.pdf (80.8 KB) 1.2_Head_of_Contracting_Activity_(HCA)_Authority,_Functions,_and_Responsibilities.pdf (25.78 KB) DOE Procurement Directors' Directory (24.94 KB) Other DOE HCA List May 26 2015.pdf (13.16 KB) 1.2_Attachment_Tables_I-IV_0.pdf (29.11 KB) 1.3_Balanced_Scorecard_Assessment_Program_0.pdf (31.8 KB) PF2015-39a - Attachment - Acq Guide Chapter

  11. Disorder-Induced Microscopic Magnetic Memory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disorder-Induced Microscopic Magnetic Memory Print The magnetic-recording industry deliberately introduces carefully controlled disorder into its materials to obtain the desired...

  12. Interface Induced Carbonate Mineralization: A Fundamental Geochemical

    Office of Scientific and Technical Information (OSTI)

    Interface Induced Carbonate Mineralization: A Fundamental Geochemical Process Relevant to Carbon Sequestration Teng, H. Henry PI, The George Washington University PI, The George...

  13. Universal solvent restructuring induced by colloidal nanoparticles...

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Search Results Journal Article: Universal solvent restructuring induced by ... Publication Date: 2015-01-15 OSTI Identifier: 1168535 Resource Type: Journal Article ...

  14. Hydrogen Adsorption Induces Interlayer Carbon Bond Formation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydrogen Adsorption Induces Interlayer Carbon Bond Formation in Supported Few-Layer ... that only a sub-monolayer amount of hydrogen adsorption on the topmost layer results ...

  15. Induced Seismicity Impact | Open Energy Information

    Open Energy Info (EERE)

    Seismicity Impact Jump to: navigation, search Retrieved from "http:en.openei.orgwindex.php?titleInducedSeismicityImpact&oldid612409" Feedback Contact needs updating...

  16. electrochemical battery stress-induced degradation mechanisms

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    electrochemical battery stress-induced degradation mechanisms - Sandia Energy Energy ... Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Fuel ...

  17. AG490 inhibits NFATc1 expression and STAT3 activation during RANKL induced osteoclastogenesis

    SciTech Connect (OSTI)

    Li, Chang-hong; Zhao, Jin-xia; Sun, Lin; Yao, Zhong-qiang; Deng, Xiao-li; Liu, Rui; Liu, Xiang-yuan

    2013-06-14

    Highlights: AG490 inhibits RANKL-induced osteoclastogenesis in RAW264.7 cells. AG490 affects cell proliferation and cell cycle distribution. AG490 reduces NFATc1 expression during RANKL-induced osteoclastogenesis. AG490 disrupts the activation of RANKL-mediated JAK2/STAT3 signaling pathway. STAT3 depletion partly mimics the effect of AG490 on RANKL-induced osteoclastogenesis. -- Abstract: Commonly, JAK/STAT relays cytokine signals for cell activation and proliferation, and recent studies have shown that the elevated expression of JAK/STAT is associated with the immune rejection of allografts and the inflammatory processes of autoimmune disease. However, the role which JAK2/STAT3 signaling plays in the receptor activator of nuclear factor-?B ligand (RANKL)-mediated osteoclastogenesis is unknown. In this study, we investigated the effects of AG490, specific JAK2 inhibitor, on osteoclast differentiation in vitro. AG490 significantly inhibited osteoclastogenesis in murine osteoclast precursor cell line RAW264.7 induced by RANKL. AG490 suppressed cell proliferation and delayed the G1 to S cell cycle transition. Furthermore, AG490 also suppressed the expression of nuclear factor of activated T cells (NFAT) c1 but not c-Fos in RAW264.7. Subsequently, we investigated various intracellular signaling components associated with osteoclastogenesis. AG490 had no effects on RANKL-induced activation of Akt, ERK1/2. Interestingly, AG490 partly inhibited RANKL-induced phosphorylation of Ser{sup 727} in STAT3. Additionally, down-regulation of STAT3 using siRNA resulted in suppression of TRAP, RANK and NFATc1 expression. In conclusion, we demonstrated that AG490 inhibited RANKL-induced osteoclastogenesis by suppressing NFATc1 production and cell proliferation via the STAT3 pathway. These results suggest that inhibition of JAK2 may be useful for the treatment of bone diseases characterized by excessive osteoclastogenesis.

  18. Cytochrome P450 2A5 and bilirubin: Mechanisms of gene regulation and cytoprotection

    SciTech Connect (OSTI)

    Kim, Sangsoo Daniel; Antenos, Monica; Squires, E. James; Kirby, Gordon M.

    2013-07-15

    Bilirubin (BR) has recently been identified as the first endogenous substrate for cytochrome P450 2A5 (CYP2A5) and it has been suggested that CYP2A5 plays a major role in BR clearance as an alternative mechanism to BR conjugation by uridine-diphosphate glucuronyltransferase 1A1. This study investigated the mechanisms of Cyp2a5 gene regulation by BR and the cytoprotective role of CYP2A5 in BR hepatotoxicity. BR induced CYP2A5 expression at the mRNA and protein levels in a dose-dependent manner in primary mouse hepatocytes. BR treatment also caused nuclear translocation of Nuclear factor-E2 p45-related factor 2 (Nrf2) in hepatocytes. In reporter assays, BR treatment of primary hepatocytes transfected with a Cyp2a5 promoter-luciferase reporter construct resulted in a 2-fold induction of Cyp2a5 reporter activity. Furthermore, cotransfection of the hepatocytes with a Nrf2 expression vector without BR treatment resulted in an increase in Cyp2a5 reporter activity of approximately 2-fold and BR treatment of Nrf2 cotransfectants further increased reporter activity by 4-fold. In addition, site-directed mutation of the ARE in the reporter construct completely abolished both the BR- and Nrf2-mediated increases in reporter activity. The cytoprotective role of CYP2A5 against BR-mediated apoptosis was also examined in Hepa 1–6 cells that lack endogenous CYP2A5. Transient overexpression of CYP2A5 partially blocked BR-induced caspase-3 cleavage in Hepa 1–6 cells. Furthermore, in vitro degradation of BR was increased by microsomes from Hepa 1–6 cells overexpressing CYP2A5 compared to control cells transfected with an empty vector. Collectively, these results suggest that Nrf2-mediated CYP2A5 transactivation in response to BR may provide an additional mechanism for adaptive cytoprotection against BR hepatotoxicity. - Highlights: • The mechanism of Cyp2a5 gene regulation by BR was investigated. • The cytoprotective role of CYP2A5 in BR hepatotoxicity was determined. • BR

  19. OAR 632-020 - Geothermal Regulations | Open Energy Information

    Open Energy Info (EERE)

    - Geothermal RegulationsLegal Published NA Year Signed or Took Effect 1972 Legal Citation OAR 632-020 (1972) DOI Not Provided Check for DOI availability: http:crossref.org...

  20. Staff Listing - Office of Regulation and International Engagement...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DC 20585 Director of the Office of Regulation and International Engagement John A. Anderson, Director Room 3E-052 Telephone (202) 586-0521 FAX (202) 586-6050 Division of...

  1. Oak Ridge EM Program Collaborates with Regulators on Groundwater Strategy

    Office of Energy Efficiency and Renewable Energy (EERE)

    OAK RIDGE, Tenn. – The Oak Ridge EM program has joined state and federal regulators in a series of workshops to address contaminated groundwater on the Oak Ridge Reservation.

  2. Title 40 Code of Federal Regulations Chapter I, Subchapter C...

    Open Energy Info (EERE)

    Page Edit with form History Title 40 Code of Federal Regulations Chapter I, Subchapter C - Air Programs Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal...

  3. Rules and Regulations for the Management and Control of Designated...

    Open Energy Info (EERE)

    the Management and Control of Designated Ground Water Jump to: navigation, search OpenEI Reference LibraryAdd to library Reference: Rules and Regulations for the Management and...

  4. Elucidating the Molecular Basis and Regulation of Chromium (VI...

    Office of Scientific and Technical Information (OSTI)

    by Shewanella oneidensis MR-1. We have identified and characterized (in the case of DNA-binding response regulator SO2426 and a putative azoreductase SO3585) the genes and ...

  5. Activation of 200 MW refusegenerated CHP upward regulation effect...

    Open Energy Info (EERE)

    EU Smart Grid Projects Map1 Overview Waste CHP plants can be used in the electricity market for upward regulation by bypassing the steam turbine. The technical design for this...

  6. Groundwater Quantity Regulation in Vermont: A Path Forward |...

    Open Energy Info (EERE)

    Groundwater Quantity Regulation in Vermont: A Path Forward Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- Secondary Legal SourceSecondary Legal...

  7. Ligand-specific regulation of the extracellular surface of a...

    Office of Scientific and Technical Information (OSTI)

    surface of a G-protein-coupled receptor Citation Details In-Document Search Title: Ligand-specific regulation of the extracellular surface of a G-protein-coupled ...

  8. Issues in federal preemption of state appliance energy efficiency regulations

    SciTech Connect (OSTI)

    Fang, J.M.; Balistocky, S.; Schaefler, A.M.

    1982-12-01

    The findings and conclusions of the analysis of the various issues involved in the federal preemption of state regulations for the DOE no standard rule on covered appliances are summarized. The covered products are: refrigerators, refrigerator-freezers, freezers, clothes dryers, kitchen ranges and ovens, water heaters (excluding heat pump water heaters), room air conditioners, central air conditioners (excluding heat pumps), and furnaces. A detailed discussion of the rationale for the positions of groups offering comment for the record is presneted. The pertinent categories of state and local regulations and programs are explained, then detailed analysis is conducted on the covered products and regulations. Issues relating to the timing of preemption of state regulations are discussed, as well as issues relating to burden of proof, contents of petitions for exemptions from preemption, criteria for evaluating petitions, and procedural and other issues. (LEW)

  9. Title 14 CCR, Division 2, Chapter 4 - Development, Regulation...

    Open Energy Info (EERE)

    4 CCR, Division 2, Chapter 4 - Development, Regulation, and Conservation of Oil and Gas Resources Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document-...

  10. Y-12 and emerging environmental regulations in 1985

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    emerging environmental regulations in 1985 In Y-12's early days of its first mission, to separate Uranium 235 from natural uranium, the peak population hit 22,482 on August 21,...

  11. Current and proposed regulations for salt water disposal wells

    SciTech Connect (OSTI)

    Moody, T.

    1994-09-01

    In recent years, all aspects of hydrocarbon exploration and production (E & P) activities have drawn closer scrutiny in terms of existing and potential impairment of the environment. In addition to drilling, production, and transportation activities, the USEPA has focused on the nature of E & P generated wastes, and the subsequent management of both hazardous and nonhazardous E & P wastes. Approximately 98% of all of the volume of wastes generated by E & P activities is salt water associated with the recovery of hydrocarbons. By far the majority of this waste is disposed of in class II salt water disposal wells. Due to the tremendous volume of salt water generated, the USEPA continues to reevaluate the federal class II salt water injection well program, offering comments, revising its interpretation of existing regulations, and promulgating new regulations. The purpose of the presentation will be to provide a review of existing class II federal regulations, and to provide an overview of potential or newly promulgated regulations.

  12. Current and proposed regulations for salt-water disposal wells

    SciTech Connect (OSTI)

    Moody, T.

    1994-12-31

    In recent years, all aspects of hydrocarbon exploration and production (E&P) activities have drawn closer scrutiny in terms of existing and potential impairment of the environment. In addition to drilling, production, and transportation activities, the United States Environmental Protection Agency (USEPA) has focused on the nature of E&P wastes. Approximately 98% of the volume of wastes generated by E&P activities is salt water associated with the recovery of hydrocarbons. By far the majority of this waste is reinjected in Class II wells as a nonhazardous waste. Due to the tremendous volume of salt water disposed of in Class II injection wells, the USEPA continues to reevaluate the Federal salt-water injection well program, offering comments, revising its interpretation of existing regulations, and promulgating new regulations. The purpose of this paper is to provide a review of existing Federal Class II injection well regulations and to provide an overview of potential of newly promulgated regulations.

  13. DOE - Office of Legacy Management -- Minneapolis-Honeywell Regulator...

    Office of Legacy Management (LM)

    Also see Documents Related to MINNEAPOLIS-HONEYWELL REGULATOR CO PA.30-1 - AEC Letter; Smith to Hughes; Subject: Uranium billet for Brown Instrument; April 11, 1952 PA.30-2 - AEC ...

  14. Global protein phosphorylation dynamics during deoxynivalenol-induced ribotoxic stress response in the macrophage

    SciTech Connect (OSTI)

    Pan, Xiao; Whitten, Douglas A.; Wu, Ming; Chan, Christina; Wilkerson, Curtis G.; Pestka, James J.

    2013-04-15

    Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium that commonly contaminates food, is capable of activating mononuclear phagocytes of the innate immune system via a process termed the ribotoxic stress response (RSR). To encapture global signaling events mediating RSR, we quantified the early temporal (≤ 30 min) phosphoproteome changes that occurred in RAW 264.7 murine macrophage during exposure to a toxicologically relevant concentration of DON (250 ng/mL). Large-scale phosphoproteomic analysis employing stable isotope labeling of amino acids in cell culture (SILAC) in conjunction with titanium dioxide chromatography revealed that DON significantly upregulated or downregulated phosphorylation of 188 proteins at both known and yet-to-be functionally characterized phosphosites. DON-induced RSR is extremely complex and goes far beyond its prior known capacity to inhibit translation and activate MAPKs. Transcriptional regulation was the main target during early DON-induced RSR, covering over 20% of the altered phosphoproteins as indicated by Gene Ontology annotation and including transcription factors/cofactors and epigenetic modulators. Other biological processes impacted included cell cycle, RNA processing, translation, ribosome biogenesis, monocyte differentiation and cytoskeleton organization. Some of these processes could be mediated by signaling networks involving MAPK-, NFκB-, AKT- and AMPK-linked pathways. Fuzzy c-means clustering revealed that DON-regulated phosphosites could be discretely classified with regard to the kinetics of phosphorylation/dephosphorylation. The cellular response networks identified provide a template for further exploration of the mechanisms of trichothecenemycotoxins and other ribotoxins, and ultimately, could contribute to improved mechanism-based human health risk assessment. - Highlights: ► Mycotoxin deoxynivalenol (DON) induces immunotoxicity via ribotoxic stress response. ► SILAC phosphoproteomics using

  15. Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells

    SciTech Connect (OSTI)

    Sassoli, Chiara; Nosi, Daniele; Tani, Alessia; Chellini, Flaminia; Mazzanti, Benedetta; Quercioli, Franco; Zecchi-Orlandini, Sandra; Formigli, Lucia

    2014-05-01

    Recent studies indicate that mesenchymal stromal cell (MSC) transplantation improves healing of injured and diseased skeletal muscle, although the mechanisms of benefit are poorly understood. In the present study, we investigated whether MSCs and/or their trophic factors were able to regulate matrix metalloproteinase (MMP) expression and activity in different cells of the muscle tissue. MSCs in co-culture with C2C12 cells or their conditioned medium (MSC-CM) up-regulated MMP-2 and MMP-9 expression and function in the myoblastic cells; these effects were concomitant with the down-regulation of the tissue inhibitor of metalloproteinases (TIMP)-1 and -2 and with increased cell motility. In the single muscle fiber experiments, MSC-CM administration increased MMP-2/9 expression in Pax-7{sup +} satellite cells and stimulated their mobilization, differentiation and fusion. The anti-fibrotic properties of MSC-CM involved also the regulation of MMPs by skeletal fibroblasts and the inhibition of their differentiation into myofibroblasts. The treatment with SB-3CT, a potent MMP inhibitor, prevented in these cells, the decrease of ?-smooth actin and type-I collagen expression induced by MSC-CM, suggesting that MSC-CM could attenuate the fibrogenic response through mechanisms mediated by MMPs. Our results indicate that growth factors and cytokines released by these cells may modulate the fibrotic response and improve the endogenous mechanisms of muscle repair/regeneration. - Highlights: MSC-CM contains paracrine factors that up-regulate MMP expression and function in different skeletal muscle cells. MSC-CM promotes myoblast and satellite cell migration, proliferation and differentiation. MSC-CM negatively interferes with fibroblast-myoblast transition in primary skeletal fibroblasts. Paracrine factors from MSCs modulate the fibrotic response and improve the endogenous mechanisms of muscle regeneration.

  16. Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Subpart A - General Provisions | Department of Energy PART 835-OCCUPATIONAL RADIATION PROTECTION Subpart A - General Provisions Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION Subpart A - General Provisions The rules in this part establish radiation protection standards, limits, and program requirements for protecting individuals from ionizing radiation resulting from the conduct of DOE activities. Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION

  17. Comparing Emissions Benefits from Regulating Heavy Vehicle Idling |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Emissions Benefits from Regulating Heavy Vehicle Idling Comparing Emissions Benefits from Regulating Heavy Vehicle Idling 2005 Diesel Engine Emissions Reduction (DEER) Conference Presentations and Posters 2005_deer_gaines.pdf (2.42 MB) More Documents & Publications Future Diesel Engine Thermal Efficiency Improvement andn Emissions Control Technology 3-D Printed Molds Hold Promise for Enhanced Wind Energy Manufacturing 3-D Printed Molds Hold Promise for Enhanced Wind

  18. Department of Energy Acquisition Regulation (DEAR) Subpart 903.9:

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Whistleblower Protections for Contractor Employees | Department of Energy Department of Energy Acquisition Regulation (DEAR) Subpart 903.9: Whistleblower Protections for Contractor Employees Department of Energy Acquisition Regulation (DEAR) Subpart 903.9: Whistleblower Protections for Contractor Employees Stakeholders: DOE Contractor employees Scope: This subpart of DEAR implements the DOE Contractor Employee Protection Program as set forth at 10 CFR part 708. This establishes the criteria

  19. Compensated gain control circuit for buck regulator command charge circuit

    DOE Patents [OSTI]

    Barrett, David M.

    1996-01-01

    A buck regulator command charge circuit includes a compensated-gain control signal for compensating for changes in the component values in order to achieve optimal voltage regulation. The compensated-gain control circuit includes an automatic-gain control circuit for generating a variable-gain control signal. The automatic-gain control circuit is formed of a precision rectifier circuit, a filter network, an error amplifier, and an integrator circuit.

  20. Compensated gain control circuit for buck regulator command charge circuit

    DOE Patents [OSTI]

    Barrett, D.M.

    1996-11-05

    A buck regulator command charge circuit includes a compensated-gain control signal for compensating for changes in the component values in order to achieve optimal voltage regulation. The compensated-gain control circuit includes an automatic-gain control circuit for generating a variable-gain control signal. The automatic-gain control circuit is formed of a precision rectifier circuit, a filter network, an error amplifier, and an integrator circuit. 5 figs.

  1. A Simpler, Smarter and More Cost Effective Approach to Regulation |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy A Simpler, Smarter and More Cost Effective Approach to Regulation A Simpler, Smarter and More Cost Effective Approach to Regulation May 26, 2011 - 9:17am Addthis Sean Lev Sean Lev Acting General Counsel & Deputy General Counsel, Environment & Nuclear Programs What does this mean for me? The Department projects a more than 90% reduction in the paperwork burden imposed on recipients of the Department of Energy's financial assistance. The Department is also working

  2. Acquisition Guide Chapter 1.1 - Acquisition Regulations System - (March

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2004) | Department of Energy 1 - Acquisition Regulations System - (March 2004) Acquisition Guide Chapter 1.1 - Acquisition Regulations System - (March 2004) Guiding Principles * Authority is delegated to the maximum practical extent. * Reviews and approvals are minimized and the layering of review is avoided. * Participants in the acquisition process work together as a team and are empowered to make decisions in the areas of responsibility. OPAM Policy Acquisition Guides (80.8 KB) More

  3. Ligand-specific regulation of the extracellular surface of a

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    G-protein-coupled receptor (Journal Article) | SciTech Connect Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor Citation Details In-Document Search Title: Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor G-protein-coupled receptors (GPCRs) are seven-transmembrane proteins that mediate most cellular responses to hormones and neurotransmitters. They are the largest group of therapeutic targets for a broad spectrum of

  4. Plants found to regulate leaf temperature to boost carbon uptake

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Plants found to regulate leaf temperature to boost carbon uptake Plants found to regulate leaf temperature to boost carbon uptake The research offers promise for refining Earth system models that help predict climate change impacts and feedbacks. August 30, 2016 The thermal traits of a leaf, critical for photosynthesis, may be under strong evolutionary selection that occurs in response to environmental temperatures. Here a thermal leaf image details temperature variation, which greatly affects

  5. Regulation Identifier Number Title/Subject/Purpose Rule Type

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7/21/2016. Highlighted areas denote changes from the last update. Regulation Identifier Number Title/Subject/Purpose Rule Type Status 1990-AA40 Adminstrative Requirements for Other Transactions: revise requirements for technology investment agreements to broaden to support all types of other transactions. NOPR Drafting Notice of Proposed Rule-making for Federal Register 1901-AB37 Enforcement of Classified Information Security Requirements: Amend DOE's statutory prescribed regulation, which set

  6. Regulations.gov - Proposed Rule Document | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations.gov - Proposed Rule Document Regulations.gov - Proposed Rule Document In response to your phone call and e-mail below the proposed rule that you reference below regarding energy efficiency standards for manufactured housing has not been issued yet by the Department of Energy (DOE) and therefore we are still in the process of drafting a proposed rule and any necessary definitions. Proposed Rule Email (197.68 KB) More Documents & Publications ISSUANCE 2015-02-04: Energy

  7. Report an Appliance Regulation Violation | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Report an Appliance Regulation Violation Report an Appliance Regulation Violation The Appliance & Equipment Standards Program in the Office of Energy Efficiency and Renewable Energy sets performance standards for the energy efficiency of many types of consumer products and industrial equipment. Report Violations To report potential violations of those standards, send an email to: energyefficiencyenforcement@hq.doe.gov or call 202-287-6997. Include in your message the type of product and the

  8. A Regulator's Perspective on Interpretation of Performance and Risk

    Office of Environmental Management (EM)

    Assessment Results | Department of Energy Interpretation of Performance and Risk Assessment Results A Regulator's Perspective on Interpretation of Performance and Risk Assessment Results Presentation from the 2015 Annual Performance and Risk Assessment (P&RA) Community of Practice (CoP) Technical Exchange Meeting held in Richland, Washington on December 15-16, 2015. A Regulator's Perspective on Interpretation of Performance and Risk Assessment Results (2.19 MB) More Documents &

  9. NNSA Updates Export Control Regulation | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration | (NNSA) Updates Export Control Regulation February 24, 2015 WASHINGTON, D.C. - The National Nuclear Security Administration (NNSA) updated the Federal rule (10 CFR Part 810, or Part 810) that regulates the export of unclassified nuclear technology and assistance. The final rule was published in the Federal Register on Feb. 23 and will go into effect on March 25, 2015. Part 810 enables civil nuclear trade by ensuring that nuclear technology and assistance exported from the

  10. DOE Proposes Regulations for Loan Guarantee Program | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Regulations for Loan Guarantee Program DOE Proposes Regulations for Loan Guarantee Program May 10, 2007 - 12:45pm Addthis Program to spur clean, innovative energy technologies WASHINGTON, DC - The U.S. Department of Energy (DOE) today issued a Notice of Proposed Rulemaking (NOPR) for its Loan Guarantee program, which will help spur investment in projects that employ new, clean energy technologies. Projects within DOE's Loan Guarantee program will help sustain economic growth, yield environmental

  11. Medical and biofuel advances possible with new gene regulation tool

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Medical and biofuel advances possible with new gene regulation tool Medical and biofuel advances possible with new gene regulation tool The key is a tunable switch made from a small non-coding RNA molecule that could have value for medical and even biofuel production purposes. August 20, 2015 Scientists at Los Alamos National Laboratory have manufactured molecular "dimmer" switches to control cellular metabolism with exquisite precision. The research has potential widespread

  12. Technical Demonstration of 2010 Emissions Regulations over Transient

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Operation | Department of Energy Demonstration of 2010 Emissions Regulations over Transient Operation Technical Demonstration of 2010 Emissions Regulations over Transient Operation Presentation given at DEER 2006, August 20-24, 2006, Detroit, Michigan. Sponsored by the U.S. DOE's EERE FreedomCar and Fuel Partnership and 21st Century Truck Programs. 2006_deer_aneja.pdf (722.19 KB) More Documents & Publications Heavy-Duty Engine Technology for High Thermal Efficiency at EPA 2010 Emissions

  13. Code of Federal Regulations Nuclear Activities | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Code of Federal Regulations Nuclear Activities Code of Federal Regulations Nuclear Activities April 24,2010 This part sets forth the procedures to govern the conduct of persons involved in DOE nuclear activities and, in particular, to achieve compliance with the DOE Nuclear Safety Requirements by all persons subject to those requirements. 10 C.F.R. 820, Procedural Rules for DOE Nuclear Activities, sets forth the procedures to implement the provisions of the Price-Anderson Amendments Act of 1988

  14. Collegiate Wind Competition 2016 Rules and Regulations | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Collegiate Wind Competition 2016 Rules and Regulations Collegiate Wind Competition 2016 Rules and Regulations The competition challenges interdisciplinary teams of undergraduate students from a variety of academic programs to offer unique solutions to complex wind-energy-related problems. To fulfill the requirements, each team must perform the following multifaceted tasks: Develop and deliver a market-research-supported business plan that shapes the design and development of the

  15. QB1 - Stochastic Gene Regulation (Conference) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    Conference: QB1 - Stochastic Gene Regulation Citation Details In-Document Search Title: QB1 - Stochastic Gene Regulation Summaries of this presentation are: (1) Stochastic fluctuations or 'noise' is present in the cell - Random motion and competition between reactants, Low copy, quantization of reactants, Upstream processes; (2) Fluctuations may be very important - Cell-to-cell variability, Cell fate decisions (switches), Signal amplification or damping, stochastic resonances; and (3) Some tools

  16. California Regulations on Renewble Hydrogen and Low Carbon Technologies |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Regulations on Renewble Hydrogen and Low Carbon Technologies California Regulations on Renewble Hydrogen and Low Carbon Technologies Presentation at the Renewable Hydrogen Workshop, Nov. 16, 2009, in Palm Springs, CA renewable_hydrogen_workshop_nov16_achtelik.pdf (561.04 KB) More Documents & Publications Transportation and Stationary Power Integration Workshop: A California Perspective Vision for Rollout of Fuel Cell Vehicles and Hydrogen Fuel Stations State of the

  17. Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy PART 835-OCCUPATIONAL RADIATION PROTECTION Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION General. The rules in this part establish radiation protection standards, limits, and program requirements for protecting individuals from ionizing radiation resulting from the conduct of DOE activities. Code of Federal Regulations PART 835-OCCUPATIONAL RADIATION PROTECTION (127.39 KB) More Documents & Publications Order Module--NNSA OCCUPATIONAL

  18. Comments Received on Proposed Rulemaking for regulation implementing

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    section 216(h): Coordination of Federal Transmission Permitting on Federal Lands | Department of Energy Received on Proposed Rulemaking for regulation implementing section 216(h): Coordination of Federal Transmission Permitting on Federal Lands Comments Received on Proposed Rulemaking for regulation implementing section 216(h): Coordination of Federal Transmission Permitting on Federal Lands Section 1221(a) of EPAct 2005, which added section 216(h) to the Federal Power Act, stated that the

  19. Hazardous waste identification: A guide to changing regulations

    SciTech Connect (OSTI)

    Stults, R.G. )

    1993-03-01

    The Resource Conservation and Recovery Act (RCRA) was enacting in 1976 and amended in 1984 by the Hazardous and Solid Waste Amendments (HSWA). Since then, federal regulations have generated a profusion of terms to identify and describe hazardous wastes. Regulations that5 define and govern management of hazardous wastes are codified in Title 40 of the code of Federal Regulations, Protection of the environment''. Title 40 regulations are divided into chapters, subchapters and parts. To be defined as hazardous, a waste must satisfy the definition of solid waste any discharged material not specifically excluded from regulation or granted a regulatory variance by the EPA Administrator. Some wastes and other materials have been identified as non-hazardous and are listed in 40 CFR 261.4(a) and 261.4(b). Certain wastes that satisfy the definition of hazardous waste nevertheless are excluded from regulation as hazardous if they meet specific criteria. Definitions and criteria for their exclusion are found in 40 CFR 261.4(c)-(f) and 40 CFR 261.5.

  20. Rab proteins: The key regulators of intracellular vesicle transport

    SciTech Connect (OSTI)

    Bhuin, Tanmay; Roy, Jagat Kumar

    2014-10-15

    Vesicular/membrane trafficking essentially regulates the compartmentalization and abundance of proteins within the cells and contributes in many signalling pathways. This membrane transport in eukaryotic cells is a complex process regulated by a large and diverse array of proteins. A large group of monomeric small GTPases; the Rabs are essential components of this membrane trafficking route. Most of the Rabs are ubiquitously expressed proteins and have been implicated in vesicle formation, vesicle motility/delivery along cytoskeleton elements and docking/fusion at target membranes through the recruitment of effectors. Functional impairments of Rabs affecting transport pathways manifest different diseases. Rab functions are accompanied by cyclical activation and inactivation of GTP-bound and GDP-bound forms between the cytosol and membranes which is regulated by upstream regulators. Rab proteins are characterized by their distinct sub-cellular localization and regulate a wide variety of endocytic, transcytic and exocytic transport pathways. Mutations of Rabs affect cell growth, motility and other biological processes. - Highlights: Rab proteins regulate different signalling pathways. Deregulation of Rabs is the fundamental causes of a variety of human diseases. This paper gives potential directions in developing therapeutic targets. This paper also gives ample directions for modulating pathways central to normal physiology. These are the huge challenges for drug discovery and delivery in near future.

  1. Consumer attitudes toward potentially restrictive energy conservation regulations

    SciTech Connect (OSTI)

    Merfeld, M.K.

    1984-01-01

    A sample of 893 Oregon individuals from a Western Regional Agricultural Experiment Station Project (W-159 Consequences of Energy Conservation Policies for Western Region Households) was used to analyze consumer attitudes toward potentially restrictive energy conservation regulations. Belief in the seriousness of the energy problem, a psychological measure of internal control (internality), and eight socio-demographic characteristics were examined in relation to consumer attitudes toward these regulations. Those who did not believe in the seriousness of the energy problem were over 50 years of age, male, had less than a college degree, and opposed mandatory energy conservation regulations related to home thermostat settings. Homeowners opposed the regulation requiring their homes to pass an energy audit. Opposition to regulations appeared to be related only to an individual's perception of the consequences of the regulation, and not to belief in the enregy problem or locus of control. Those with low internality scores included females, rural residents, respondents over 50, and those with less than a college degree.

  2. Induced topological pressure for topological dynamical systems

    SciTech Connect (OSTI)

    Xing, Zhitao; Chen, Ercai

    2015-02-15

    In this paper, inspired by the article [J. Jaerisch et al., Stochastics Dyn. 14, 1350016, pp. 1-30 (2014)], we introduce the induced topological pressure for a topological dynamical system. In particular, we prove a variational principle for the induced topological pressure.

  3. Solvent-induced forces in protein folding

    SciTech Connect (OSTI)

    Ben-Naim, A. (Hebrew Univ., Jerusalem (Israel))

    1990-08-23

    The solvent-induced forces between various groups on the protein are examined. It is found that the intramolecular hydrophilic forces are likely to be the strongest forces mediated through the solvent. It is argued that these are probably the most important solvent-induced driving forces in the process of protein folding.

  4. Metallothionein deficiency aggravates depleted uranium-induced nephrotoxicity

    SciTech Connect (OSTI)

    Hao, Yuhui; Huang, Jiawei; Gu, Ying; Liu, Cong; Li, Hong; Liu, Jing; Ren, Jiong; Yang, Zhangyou; Peng, Shuangqing; Wang, Weidong; Li, Rong

    2015-09-15

    Depleted uranium (DU) has been widely used in both civilian and military activities, and the kidney is the main target organ of DU during acute high-dose exposures. In this study, the nephrotoxicity caused by DU in metallothionein-1/2-null mice (MT −/−) and corresponding wild-type (MT +/+) mice was investigated to determine any associations with MT. Each MT −/− or MT +/+ mouse was pretreated with a single dose of DU (10 mg/kg, intraperitoneal injection) or an equivalent volume of saline. After 4 days of DU administration, kidney changes were assessed. After DU exposure, serum creatinine and serum urea nitrogen in MT −/− mice significantly increased than in MT +/+ mice, with more severe kidney pathological damage. Moreover, catalase and superoxide dismutase (SOD) decreased, and generation of reactive oxygen species and malondialdehyde increased in MT −/− mice. The apoptosis rate in MT −/− mice significantly increased, with a significant increase in both Bax and caspase 3 and a decrease in Bcl-2. Furthermore, sodium-glucose cotransporter (SGLT) and sodium-phosphate cotransporter (NaPi-II) were significantly reduced after DU exposure, and the change of SGLT was more evident in MT −/− mice. Finally, exogenous MT was used to evaluate the correlation between kidney changes induced by DU and MT doses in MT −/− mice. The results showed that, the pathological damage and cell apoptosis decreased, and SOD and SGLT levels increased with increasing dose of MT. In conclusion, MT deficiency aggravated DU-induced nephrotoxicity, and the molecular mechanisms appeared to be related to the increased oxidative stress and apoptosis, and decreased SGLT expression. - Highlights: • MT −/− and MT +/+ mice were used to evaluate nephrotoxicity of DU. • Renal damage was more evident in the MT −/− mice after exposure to DU. • Exogenous MT also protects against DU-induced nephrotoxicity. • MT deficiency induced more ROS and apoptosis after exposure to

  5. Traf2 interacts with Smad4 and regulates BMP signaling pathway in MC3T3-E1 osteoblasts

    SciTech Connect (OSTI)

    Shimada, Koichi; Ikeda, Kyoko; Ito, Koichi

    2009-12-18

    Bone morphogenetic proteins (BMPs) play important roles in osteoblast differentiation and maturation. In mammals, the BMP-induced receptor-regulated Smads form complexes with Smad4. These complexes translocate and accumulate in the nucleus, where they regulate the transcription of various target genes. However, the function of Smad4 remains unclear. We performed a yeast two-hybrid screen using Smad4 as bait and a cDNA library derived from bone marrow, to indentify the proteins interacting with Smad4. cDNA clones for Tumor necrosis factor (TNF) receptor-associated factor 2 (Traf2) were identified, and the interaction between the endogenous proteins was confirmed in the mouse osteoblast cell line MC3T3-E1. To investigate the function of Traf2, we silenced it with siRNA. The level of BMP-2 protein in the medium, the expression levels of the Bmp2 gene and BMP-induced transcription factor genes, including Runx2, Dlx5, Msx2, and Sp7, and the phosphorylated-Smad1 protein level were increased in cells transfected with Traf2 siRNA. The nuclear accumulation of Smad1 increased with TNF-{alpha} stimulation for 30 min at Traf2 silencing. These results suggest that the TNF-{alpha}-stimulated nuclear accumulation of Smad1 may be dependent on Traf2. Thus, the interaction between Traf2 and Smad4 may play a role in the cross-talk between TNF-{alpha} and BMP signaling pathways.

  6. Global identification of genes regulated by estrogen signaling and demethylation in MCF-7 breast cancer cells

    SciTech Connect (OSTI)

    Putnik, Milica; Zhao, Chunyan; Gustafsson, Jan-Ake; Department of Biology and Biochemistry, Science and Engineering Research Center Bldg, University of Houston, Houston, TX 77204-5056 ; Dahlman-Wright, Karin

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer Estrogen signaling and demethylation can both control gene expression in breast cancers. Black-Right-Pointing-Pointer Cross-talk between these mechanisms is investigated in human MCF-7 breast cancer cells. Black-Right-Pointing-Pointer 137 genes are influenced by both 17{beta}-estradiol and demethylating agent 5-aza-2 Prime -deoxycytidine. Black-Right-Pointing-Pointer A set of genes is identified as targets of both estrogen signaling and demethylation. Black-Right-Pointing-Pointer There is no direct molecular interplay of mediators of estrogen and epigenetic signaling. -- Abstract: Estrogen signaling and epigenetic modifications, in particular DNA methylation, are involved in regulation of gene expression in breast cancers. Here we investigated a potential regulatory cross-talk between these two pathways by identifying their common target genes and exploring underlying molecular mechanisms in human MCF-7 breast cancer cells. Gene expression profiling revealed that the expression of approximately 140 genes was influenced by both 17{beta}-estradiol (E2) and a demethylating agent 5-aza-2 Prime -deoxycytidine (DAC). Gene ontology (GO) analysis suggests that these genes are involved in intracellular signaling cascades, regulation of cell proliferation and apoptosis. Based on previously reported association with breast cancer, estrogen signaling and/or DNA methylation, CpG island prediction and GO analysis, we selected six genes (BTG3, FHL2, PMAIP1, BTG2, CDKN1A and TGFB2) for further analysis. Tamoxifen reverses the effect of E2 on the expression of all selected genes, suggesting that they are direct targets of estrogen receptor. Furthermore, DAC treatment reactivates the expression of all selected genes in a dose-dependent manner. Promoter CpG island methylation status analysis revealed that only the promoters of BTG3 and FHL2 genes are methylated, with DAC inducing demethylation, suggesting DNA methylation directs repression of

  7. Arsenite evokes IL-6 secretion, autocrine regulation of STAT3 signaling, and miR-21 expression, processes involved in the EMT and malignant transformation of human bronchial epithelial cells

    SciTech Connect (OSTI)

    Luo, Fei; Xu, Yuan; Ling, Min; Zhao, Yue; Xu, Wenchao; Liang, Xiao; Jiang, Rongrong; Wang, Bairu; Bian, Qian; Liu, Qizhan

    2013-11-15

    Arsenite is an established human carcinogen, and arsenite-induced inflammation contributes to malignant transformation of cells, but the molecular mechanisms by which cancers are produced remain to be established. The present results showed that, evoked by arsenite, secretion of interleukin-6 (IL-6), a pro-inflammatory cytokine, led to the activation of STAT3, a transcription activator, and to increased levels of a microRNA, miR-21. Blocking IL-6 with anti-IL-6 antibody and inhibiting STAT3 activation reduced miR-21 expression. For human bronchial epithelial cells, cultured in the presence of anti-IL-6 antibody for 3 days, the arsenite-induced EMT and malignant transformation were reversed. Thus, IL-6, acting on STAT3 signaling, which up-regulates miR-21in an autocrine manner, contributes to the EMT induced by arsenite. These data define a link from inflammation to EMT in the arsenite-induced malignant transformation of HBE cells. This link, mediated through miRNAs, establishes a mechanism for arsenite-induced lung carcinogenesis. - Highlights: Arsenite evokes IL-6 secretion. IL-6 autocrine mediates STAT3 signaling and up-regulates miR-21expression. Inflammation is involved in arsenite-induced EMT.

  8. Regulation of myosin IIA and filamentous actin during insulin-stimulated glucose uptake in 3T3-L1 adipocytes

    SciTech Connect (OSTI)

    Stall, Richard; Ramos, Joseph; Kent Fulcher, F.; Patel, Yashomati M.

    2014-03-10

    Insulin stimulated glucose uptake requires the colocalization of myosin IIA (MyoIIA) and the insulin-responsive glucose transporter 4 (GLUT4) at the plasma membrane for proper GLUT4 fusion. MyoIIA facilitates filamentous actin (F-actin) reorganization in various cell types. In adipocytes F-actin reorganization is required for insulin-stimulated glucose uptake. What is not known is whether MyoIIA interacts with F-actin to regulate insulin-induced GLUT4 fusion at the plasma membrane. To elucidate the relationship between MyoIIA and F-actin, we examined the colocalization of MyoIIA and F-actin at the plasma membrane upon insulin stimulation as well as the regulation of this interaction. Our findings demonstrated that MyoIIA and F-actin colocalized at the site of GLUT4 fusion with the plasma membrane upon insulin stimulation. Furthermore, inhibition of MyoII with blebbistatin impaired F-actin localization at the plasma membrane. Next we examined the regulatory role of calcium in MyoIIA-F-actin colocalization. Reduced calcium or calmodulin levels decreased colocalization of MyoIIA and F-actin at the plasma membrane. While calcium alone can translocate MyoIIA it did not stimulate F-actin accumulation at the plasma membrane. Taken together, we established that while MyoIIA activity is required for F-actin localization at the plasma membrane, it alone is insufficient to localize F-actin to the plasma membrane. - Highlights: • Insulin induces colocalization of MyoIIA and F-actin at the cortex in adipocytes. • MyoIIA is necessary but not sufficient to localize F-actin at the cell cortex. • MyoIIA-F-actin colocalization is regulated by calcium and calmodulin.

  9. Colorado 4 CCR 723-3001, Definitions for Rules Regulating Electric...

    Open Energy Info (EERE)

    4 CCR 723-3001, Definitions for Rules Regulating Electric Utilities Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  10. 4 Code of Colorado Regulations (CCR) 723-3 | Open Energy Information

    Open Energy Info (EERE)

    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 4 Code of Colorado Regulations (CCR) 723-3Legal Abstract Colorado Public Utilities...

  11. NMAC 1.2.2 Public Regulations Commission Rules of Procedure ...

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    NMAC 1.2.2 Public Regulations Commission Rules of Procedure Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: NMAC 1.2.2...

  12. 1 NMAC 2.2 - Public Regulation Commission Rules of Procedure...

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    NMAC 2.2 - Public Regulation Commission Rules of Procedure Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: 1 NMAC 2.2 -...

  13. Code of Colorado Regulations 2 CCR 601-1, State Highway Access...

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    Code of Colorado Regulations 2 CCR 601-1, State Highway Access Code Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: Code of...

  14. Colorado Code of Regulations 2 CCR 601-18, State Utility Accommodation...

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    Colorado Code of Regulations 2 CCR 601-18, State Utility Accommodation Code Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation:...

  15. H.A.R. 13-171 - Designation and Regulation of Water Management...

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    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: H.A.R. 13-171 - Designation and Regulation of Water Management AreasLegal Published NA Year...

  16. WAC 332-17 Geothermal Drilling Rules and Regulations | Open Energy...

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    17 Geothermal Drilling Rules and Regulations Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC 332-17 Geothermal Drilling...

  17. O.A.R. 340-045 - Regulations Pertaining to NPDES and WPCF Permits...

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    search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: O.A.R. 340-045 - Regulations Pertaining to NPDES and WPCF PermitsLegal Abstract These...

  18. Cell recognition molecule L1 promotes embryonic stem cell differentiation through the regulation of cell surface glycosylation

    SciTech Connect (OSTI)

    Li, Ying; Department of Clinical Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian 116023 ; Huang, Xiaohua; Department of Clinical Biochemistry, College of Laboratory Medicine, Dalian Medical University, Dalian 116044 ; An, Yue; Ren, Feng; Yang, Zara Zhuyun; Zhu, Hongmei; Zhou, Lei; Department of Anatomy and Developmental Biology, Monash University, Clayton 3800 ; He, Xiaowen; Schachner, Melitta; Xiao, Zhicheng; Department of Anatomy and Developmental Biology, Monash University, Clayton 3800 ; Ma, Keli; Li, Yali; Department of Anatomy, National University of Singapore, Singapore 119078

    2013-10-25

    Highlights: Down-regulating FUT9 and ST3Gal4 expression blocks L1-induced neuronal differentiation of ESCs. Up-regulating FUT9 and ST3Gal4 expression in L1-ESCs depends on the activation of PLC?. L1 promotes ESCs to differentiate into neuron through regulating cell surface glycosylation. -- Abstract: Cell recognition molecule L1 (CD171) plays an important role in neuronal survival, migration, differentiation, neurite outgrowth, myelination, synaptic plasticity and regeneration after injury. Our previous study has demonstrated that overexpressing L1 enhances cell survival and proliferation of mouse embryonic stem cells (ESCs) through promoting the expression of FUT9 and ST3Gal4, which upregulates cell surface sialylation and fucosylation. In the present study, we examined whether sialylation and fucosylation are involved in ESC differentiation through L1 signaling. RNA interference analysis showed that L1 enhanced differentiation of ESCs into neurons through the upregulation of FUT9 and ST3Gal4. Furthermore, blocking the phospholipase C? (PLC?) signaling pathway with either a specific PLC? inhibitor or knockdown PLC? reduced the expression levels of both FUT9 and ST3Gal4 mRNAs and inhibited L1-mediated neuronal differentiation. These results demonstrate that L1 promotes neuronal differentiation from ESCs through the L1-mediated enhancement of FUT9 and ST3Gal4 expression.

  19. Inducible secretion of a cellulase from Clostridium thermocellum in Bacillus subtilis

    SciTech Connect (OSTI)

    Joliff, G.; Edelman, A., Klier, A.; Rapoport, G. )

    1989-11-01

    A host-vector system for inducible secretion during the logarithmic growth phase in Bacillus subtilis has been developed. The B. subtilis levansucrase gene promoter and the region encoding its signal sequence have been used. The endoglucanase A of Clostridium thermocellum was used as a model protein to test the efficiency of the system. Effective inducible secretion of the endoglucanase A was observed when either the levansucrase signal sequence or its own signal sequence was used. Expression of the endoglucanase A in different genetic backgrounds of B. subtilis showed that its regulation was similar to that of levansucrase, and high enzyme activity was recovered from the culture supernatant of a hyperproducing B. subtilis sacU(Hy) strain. The molecular weight of 46,000 estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the secreted endoglucanase A is compatible with the calculated molecular weight of the mature polypeptide.

  20. Naphthazarin protects against glutamate-induced neuronal death via activation of the Nrf2/ARE pathway

    SciTech Connect (OSTI)

    Son, Tae Gen; Kawamoto, Elisa M.; Yu, Qian-Sheng; Greig, Nigel H.; Mattson, Mark P.; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD ; Camandola, Simonetta

    2013-04-19

    Highlights: Naphthazarin activates the Nrf2/ARE pathway. Naphthazarin induces Nrf2-driven genes in neurons and astrocytes. Naphthazarin protects neurons against excitotoxicity. -- Abstract: Nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway is an important cellular stress response pathway involved in neuroprotection. We previously screened several natural phytochemicals and identified plumbagin as a novel activator of the Nrf2/ARE pathway that can protect neurons against ischemic injury. Here we extended our studies to natural and synthetic derivatives of plumbagin. We found that 5,8-dimethoxy-1,4-naphthoquinone (naphthazarin) is a potent activator of the Nrf2/ARE pathway, up-regulates the expression of Nrf2-driven genes in primary neuronal and glial cultures, and protects neurons against glutamate-induced excitotoxicity.