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Sample records for union gdp gross

  1. Fact #564: March 30, 2009 Transportation and the Gross Domestic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Housing, health care, and food are the only categories with greater shares of the GDP. GDP ... Gross Domestic Product, 2007 Housing 24.3% Health Care 17.4% Food 11.6% ...

  2. Fact #564: March 30, 2009 Transportation and the Gross Domestic Product,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2007 | Department of Energy 4: March 30, 2009 Transportation and the Gross Domestic Product, 2007 Fact #564: March 30, 2009 Transportation and the Gross Domestic Product, 2007 Transportation plays a major role in the U.S. economy. About 10% of the U.S. Gross Domestic Product (GDP) in 2007 is related to transportation. Housing, health care, and food are the only categories with greater shares of the GDP. GDP by Category, 2007 Graph showing the Gross Domestic Product (GDP) for various

  3. Fact# 904: December 21, 2015 Gross Domestic Product and Vehicle...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    With the growth of VMT in 2015, the gap between the two series has narrowed for the first time since the Great Recession. GDP and VMT Trends, 1960-2015 Graph showing gross national ...

  4. Samantha Gross

    Broader source: Energy.gov [DOE]

    Samantha Gross is the Director for International Climate and Clean Energy at the Office of International Affairs in the U.S. Department of Energy. She directs U.S. activities under the Clean Energy...

  5. New Contract Helps Portsmouth GDP Cleanup

    Broader source: Energy.gov [DOE]

    To accelerate the Portsmouth GDP cleanup efforts left over from the Cold War, the Department of Energy made a huge step forward in our nuclear environmental cleanup efforts.

  6. Accommodation of GDP-Linked Sugars in the Active Site of GDP-Perosamine Synthase

    SciTech Connect (OSTI)

    Cook, Paul D.; Carney, Amanda E.; Holden, Hazel M.

    2009-01-12

    Perosamine (4-amino-4,6-dideoxy-d-mannose), or its N-acetylated form, is one of several dideoxy sugars found in the O-antigens of such infamous Gram-negative bacteria as Vibrio cholerae O1 and Escherichia coli O157:H7. It is added to the bacterial O-antigen via a nucleotide-linked version, namely GDP-perosamine. Three enzymes are required for the biosynthesis of GDP-perosamine starting from mannose 1-phosphate. The focus of this investigation is GDP-perosamine synthase from Caulobacter crescentus, which catalyzes the final step in GDP-perosamine synthesis, the conversion of GDP-4-keto-6-deoxymannose to GDP-perosamine. The enzyme is PLP-dependent and belongs to the aspartate aminotransferase superfamily. It contains the typically conserved active site lysine residue, which forms a Schiff base with the PLP cofactor. Two crystal structures were determined for this investigation: a site-directed mutant protein (K186A) complexed with GDP-perosamine and the wild-type enzyme complexed with an unnatural ligand, GDP-3-deoxyperosamine. These structures, determined to 1.6 and 1.7 {angstrom} resolution, respectively, revealed the manner in which products, and presumably substrates, are accommodated within the active site pocket of GDP-perosamine synthase. Additional kinetic analyses using both the natural and unnatural substrates revealed that the K{sub m} for the unnatural substrate was unperturbed relative to that of the natural substrate, but the k{sub cat} was lowered by a factor of approximately 200. Taken together, these studies shed light on why GDP-perosamine synthase functions as an aminotransferase whereas another very similar PLP-dependent enzyme, GDP-4-keto-6-deoxy-d-mannose 3-dehydratase or ColD, catalyzes a dehydration reaction using the same substrate.

  7. Fact# 904: December 21, 2015 Gross Domestic Product and Vehicle Travel:

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Both Increased during 2015 | Department of Energy 4: December 21, 2015 Gross Domestic Product and Vehicle Travel: Both Increased during 2015 Fact# 904: December 21, 2015 Gross Domestic Product and Vehicle Travel: Both Increased during 2015 SUBSCRIBE to the Fact of the Week The nation's highway vehicle miles of travel (VMT) and the U.S. gross domestic product (GDP) reflect strikingly similar patterns, indicating the strong relationship between the nation's economy and its travel. Beginning in

  8. What is Gross Up?

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the payment if we had not paid you the additional amount. For example: If the Relocation reimbursement request submitted 5668. Without a gross up the net payment received ...

  9. grossWCI.dvi

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear multifragmentation, Its relation to general physics A rich test-ground of the fundamentals of statistical mechanics. D.H.E. Gross 1 Hahn-Meitner Institute Glienickerstr. 100 14109 Berlin, Germany gross@hmi.de; http://www.hmi.de/people/gross/ 2 Freie Universit¨ at Berlin, Fachbereich Physik. Received: date / Revised version: date Abstract. Heat can flow from cold to hot at any phase separation, even in macroscopic systems. Therefore also Lynden-Bell's famous gravo-thermal catastrophe [1]

  10. Fact #768: February 25, 2013 New Light Vehicle Sales and Gross Domestic

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Product | Department of Energy 8: February 25, 2013 New Light Vehicle Sales and Gross Domestic Product Fact #768: February 25, 2013 New Light Vehicle Sales and Gross Domestic Product Over the last four decades, new light vehicle sales have gone from a low of 9.9 million vehicles in 1970 to a high of 17.1 million vehicles sold in 2001, but along the way, there have been significant ups and downs. Those ups and downs are also reflected in the change in Gross Domestic Product (GDP) over time

  11. Agreement Type Union

    National Nuclear Security Administration (NNSA)

    Union of North America 872 14 International Union of Oprating Engineers (Maintenance Engineers) 501 22 Local Joint Executive Board of Las vegas Culinary Workers 226 49 ...

  12. Enel Union Fenosa Renovables EUFER formerly Union Fenosa Energia...

    Open Energy Info (EERE)

    Fenosa Renovables EUFER formerly Union Fenosa Energia Especiales Jump to: navigation, search Name: Enel Union Fenosa Renovables (EUFER) (formerly Union Fenosa Energia Especiales)...

  13. Structures of NodZ ?1,6-fucosyltransferase in complex with GDP and GDP-fucose

    SciTech Connect (OSTI)

    Brzezinski, Krzysztof; Dauter, Zbigniew; Jaskolski, Mariusz

    2012-02-01

    Crystal structures of the bacterial ?1,6-fucosyltransferase NodZ in complex with GDP and GDP-fucose are presented. Rhizobial NodZ ?1,6-fucosyltransferase (?1,6-FucT) catalyzes the transfer of the fucose (Fuc) moiety from guanosine 5?-diphosphate-?-l-fucose to the reducing end of the chitin oligosaccharide core during Nod-factor (NF) biosynthesis. NF is a key signalling molecule required for successful symbiosis with a legume host for atmospheric nitrogen fixation. To date, only two ?1,6-FucT structures have been determined, both without any donor or acceptor molecule that could highlight the structural background of the catalytic mechanism. Here, the first crystal structures of ?1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the enzymatic reaction, are presented. The crystal of the complex with GDP-Fuc was obtained through soaking of native NodZ crystals with the ligand and its structure has been determined at 2.35 Ĺ resolution. The fucose residue is exposed to solvent and is disordered. The enzyme–product complex crystal was obtained by cocrystallization with GDP and an acceptor molecule, penta-N-acetyl-l-glucosamine (penta-NAG). The structure has been determined at 1.98 Ĺ resolution, showing that only the GDP molecule is present in the complex. In both structures the ligands are located in a cleft formed between the two domains of NodZ and extend towards the C-terminal domain, but their conformations differ significantly. The structures revealed that residues in three regions of the C-terminal domain, which are conserved among ?1,2-, ?1,6- and protein O-fucosyltransferases, are involved in interactions with the sugar-donor molecule. There is also an interaction with the side chain of Tyr45 in the N-terminal domain, which is very unusual for a GT-B-type glycosyltransferase. Only minor conformational changes of the protein backbone are observed upon ligand binding. The only exception is a movement of the loop located between

  14. ,"Alaska Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    "Back to Contents","Data 1: Alaska Natural Gas Gross Withdrawals and Production" ... "Date","Alaska Natural Gas Gross Withdrawals (MMcf)","Alaska Natural ...

  15. Oil-Consumption-Weighted GDP: Description, Calculation, and Comparison

    U.S. Energy Information Administration (EIA) Indexed Site

    Oil-Consumption-Weighted GDP: Description, Calculation, and Comparison Vipin Arora, Tyler Hodge, and Tancred Lidderdale May 2016 Independent Statistics & Analysis www.eia.gov U.S. Energy Information Administration Washington, DC 20585 This paper is released to encourage discussion and critical comment. The analysis and conclusions expressed here are those of the authors and not necessarily those of the U.S. Energy Information Administration. Our thanks to Vinay Tripathi, summer intern from

  16. Structures of NodZ [alpha]1,6-fucosyltransferase in complex with GDP and GDP-fucose

    SciTech Connect (OSTI)

    Brzezinski, Krzysztof; Dauter, Zbigniew; Jaskolski, Mariusz

    2012-03-26

    Rhizobial NodZ {alpha}1,6-fucosyltransferase ({alpha}1,6-FucT) catalyzes the transfer of the fucose (Fuc) moiety from guanosine 5'-diphosphate-{beta}-L-fucose to the reducing end of the chitin oligosaccharide core during Nod-factor (NF) biosynthesis. NF is a key signaling molecule required for successful symbiosis with a legume host for atmospheric nitrogen fixation. To date, only two {alpha}1,6-FucT structures have been determined, both without any donor or acceptor molecule that could highlight the structural background of the catalytic mechanism. Here, the first crystal structures of {alpha}1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the enzymatic reaction, are presented. The crystal of the complex with GDP-Fuc was obtained through soaking of native NodZ crystals with the ligand and its structure has been determined at 2.35 {angstrom} resolution. The fucose residue is exposed to solvent and is disordered. The enzyme-product complex crystal was obtained by cocrystallization with GDP and an acceptor molecule, penta-N-acetyl-L-glucosamine (penta-NAG). The structure has been determined at 1.98 {angstrom} resolution, showing that only the GDP molecule is present in the complex. In both structures the ligands are located in a cleft formed between the two domains of NodZ and extend towards the C-terminal domain, but their conformations differ significantly. The structures revealed that residues in three regions of the C-terminal domain, which are conserved among {alpha}1,2-, {alpha}1,6- and protein O-fucosyltransferases, are involved in interactions with the sugar-donor molecule. There is also an interaction with the side chain of Tyr45 in the N-terminal domain, which is very unusual for a GT-B-type glycosyltransferase. Only minor conformational changes of the protein backbone are observed upon ligand binding. The only exception is a movement of the loop located between strand {beta}C2 and helix {alpha}C3. In addition, there is

  17. MEETINGS WITH UNION OFFICIALS

    Broader source: Energy.gov [DOE]

    Guidance as to how to handle requests from union officials to meet with Department officials so that there is no undue legal risk.

  18. ,"West Virginia Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    AM" "Back to Contents","Data 1: West Virginia Natural Gas Gross Withdrawals (MMcf)" "Sourcekey","N9010WV2" "Date","West Virginia Natural Gas Gross Withdrawals (MMcf)" ...

  19. ,"New York Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...2016 10:10:10 AM" "Back to Contents","Data 1: New York Natural Gas Gross Withdrawals (MMcf)" "Sourcekey","N9010NY2" "Date","New York Natural Gas Gross Withdrawals (MMcf)" ...

  20. ,"New Mexico Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...2016 10:10:09 AM" "Back to Contents","Data 1: New Mexico Natural Gas Gross Withdrawals (MMcf)" "Sourcekey","N9010NM2" "Date","New Mexico Natural Gas Gross Withdrawals (MMcf)" ...

  1. ,"New Mexico Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    9:51:59 AM" "Back to Contents","Data 1: New Mexico Natural Gas Gross Withdrawals (MMcf)" "Sourcekey","N9010NM2" "Date","New Mexico Natural Gas Gross Withdrawals (MMcf)" ...

  2. ,"North Dakota Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...2016 9:51:58 AM" "Back to Contents","Data 1: North Dakota Natural Gas Gross Withdrawals (MMcf)" "Sourcekey","N9010ND2" "Date","North Dakota Natural Gas Gross Withdrawals (MMcf)" ...

  3. Agreement Type Union

    National Nuclear Security Administration (NNSA)

    Type Union Local #/Name Number of Employees Project Labor Agreement International Association of Heat and Frost Insulators and Allied Workers 135 2 International Brothehood of Boilermakers, Iron Ship Builders, Blacksmith Forgers and Helpers 92 0 International Union of Bricklayers & Allied Craftsmen 13 0 Regional Council of Carpenters 1780 & 1977 13 Operative Plasterers and Cement Mason International Association Operative Plasterers and Cement Mason International Association 1

  4. Michael Gross | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Michael Gross Michael Gross Michael Gross Principal Investigator E-mail: mgross@wustl.edu Phone: (314) 935-4814 Website: Washington University in St. Louis Principal Investigator Dr. Gross's research interests include analytical chemistry, biological chemistry, biophysical chemistry, FT-ICR instrument development, MALDI matrix development, mass spectrometry for protein biochemistry and biophysics, modified DNA and cancer, physical organic chemistry, protein and peptide analysis, and proteomics.

  5. Roughness Optimization at High Modes for GDP CHx Microshells

    SciTech Connect (OSTI)

    Theobald, M.; Dumay, B.; Chicanne, C.; Barnouin, J.; Legaie, O.; Baclet, P.

    2004-03-15

    For the ''Megajoule'' Laser (LMJ) facility of the CEA, amorphous hydrogenated carbon (a-C:H) is the nominal ablator to be used for inertial confinement fusion (ICF) experiments. These capsules contain the fusible deuterium-tritium mixture to achieve ignition. Coatings are prepared by glow discharge polymerization (GDP) with trans-2-butene and hydrogen. The films properties have been investigated. Laser fusion targets must have optimized characteristics: a diameter of about 2.4 mm for LMJ targets, a thickness up to 175 {mu}m, a sphericity and a thickness concentricity better than 99% and an outer and an inner roughness lower than 20 nm at high modes. The surface finish of these laser fusion targets must be extremely smooth to minimize hydrodynamic instabilities.Movchan and Demchishin, and later Thornton introduced a structure zone model (SZM) based on both evaporated and sputtered metals. They investigated the influence of base temperature and the sputtering gas pressure on structure and properties of thick polycrystalline coatings of nickel, titanium, tungsten, aluminum oxide. An original cross-sectional analysis by atomic force microscopy (AFM) allows amorphous materials characterization and permits to make an analogy between the amorphous GDP material and the existing model (SZM). The purpose of this work is to understand the relationship between the deposition parameters, the growing structures and the surface roughness.The coating structure as a function of deposition parameters was first studied on plane silicon substrates and then optimized on PAMS shells. By adjusting the coating parameters, the structures are modified, and in some case, the high modes roughness decreases dramatically.

  6. Alaska--State Offshore Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Alaska--State Offshore Natural Gas Gross ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  7. US--Federal Offshore Natural Gas Gross Withdrawals (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) US--Federal Offshore Natural Gas Gross Withdrawals ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  8. Louisiana--State Offshore Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Louisiana--State Offshore Natural Gas Gross ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  9. Federal Offshore--Alabama Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Federal Offshore--Alabama Natural Gas Gross ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  10. Texas--State Offshore Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Texas--State Offshore Natural Gas Gross Withdrawals ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  11. Federal Offshore--Texas Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Federal Offshore--Texas Natural Gas Gross ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  12. US--State Offshore Natural Gas Gross Withdrawals (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) US--State Offshore Natural Gas Gross Withdrawals ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  13. Alabama--State Offshore Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Alabama--State Offshore Natural Gas Gross ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  14. Federal Offshore--Louisiana Natural Gas Gross Withdrawals (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Federal Offshore--Louisiana Natural Gas Gross ... Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Offshore Gross ...

  15. Motor vehicle output and GDP, 1968-2007.

    SciTech Connect (OSTI)

    Santini, D. J.; Poyer, D. A.

    2008-01-01

    In this paper, we assess the performance of the BEA series 'value of motor vehicle output' as an indicator of the business cycle over the period 1968-2007. We statistically assess the causal relationship between real motor vehicle output (RMVO) and real gross domestic product (RGDP). This is accomplished by standard estimation and statistical methods used to assess vector autoregressive models. This assessment represents the initial results of a more encompassing research project, the intent of which is to determine the dynamic interaction of the transport sector with the overall economy. It's a start to a more comprehensive assessment of how transport and economic activity interrelate.

  16. Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro

    SciTech Connect (OSTI)

    Zhao Guohui; Liu Jun; Liu Xiang; Chen Min; Zhang Houcheng Wang, Peng George

    2007-11-23

    GDP-perosamine synthetase (Per, E.C. not yet classified) is important to the synthesis of Escherichia coli O157:H7 O-antigen. The mutant in per gene can disrupt the synthesis of O157 O-antigen. In this study, GDP-perosamine synthetase was cloned from E. coli O157:H7 and over-expressed in E. coli BL21 (DE3). The recombinant His-tagged Per fusion protein was a decamer with molecular weight of 431 kDa. The optimal pH value of this recombinant protein was 7.5. The divalent ions had no significant effect on Per-catalyzed reaction. The K{sub m} and K{sub cat}/K{sub m} for GDP-4-keto-6-deoxy-D-mannose were 0.09 mM and 2.1 x 10{sup 5} M{sup -1} S{sup -1}, and those for L-glutamate were 2 mM and 0.52 x 10{sup 5} M{sup -1}S{sup -1}, respectively. Per was used to synthesize GDP-perosamine from GDP-mannose together with recombinant GDP-mannose dehydratase (GMD, E.C. 4.2.1.47). The purified GDP-perosamine was identified by MS and NMR. In summary, this work provided a feasible approach for the synthesis of GDP-perosamine which can lead to the study of LPS biosynthesis of pathogenic E. coli O157:H7.

  17. Mechanical Properties of Thin GDP Shells Used as Cryogenic Direct Drive Targets at OMEGA

    SciTech Connect (OSTI)

    Nikroo, A.; Czechowicz, D.G.; Chen, K.C.; Dicken, M.; Morris, C.; Andrews, R.; Greenwood, A.; Castillo, E.

    2004-03-15

    Thin glow discharge polymer (GDP) shells are currently used as the targets for cryogenic direct drive laser fusion experiments. These shells need to be filled with nearly 1000 atm of D{sub 2} and cooled to cryogenic temperatures without failing due to buckling and bursting pressures they experience in this process. Therefore, the mechanical and permeation properties of these shells are of utmost importance in successful and rapid filling with D{sub 2}. In this paper, we present an overview of buckle and burst pressures of several different types of GDP shells. These include those made using traditional GDP deposition parameters ('standard GDP') using a high deposition pressure and using modified parameters ('strong GDP') of low deposition pressure that leads to more robust shells.

  18. MECHANICAL PROPERTIES OF THIN GDP SHELLS USED AS CRYOGENIC DIRECT DRIVE TARGETS AT OMEGA

    SciTech Connect (OSTI)

    NIKROO,A; CZECHOWICZ,D; CHEN,K.C; DICKEN,M; MORRIS,C; ANDREWS,R; GREENWOOD,A.L; CASTILLO,E

    2003-06-01

    OAK-B135 Thin glow discharge polymer (GDP) shells are currently used as the targets for cryogenic direct drive laser fusion experiments. These shells need to be filled with nearly 1000 atm of D{sub 2} and cooled to cryogenic temperatures without failing due to buckling and bursting pressures they experience in this process. Therefore, the mechanical and permeation properties of these shells are of utmost importance in successful and rapid filling with D{sub 2}. In this paper, they present an overview of buckle and burst pressures of several different types of GDP shells. These include those made using traditional GDP deposition parameters (standard GDP) using a high deposition pressure and using modified parameters (strong GDP) of low deposition pressure that leads to more robust shells.

  19. Biochemical characterization of GDP-L-fucose de novo synthesis pathway in fungus Mortierella alpina

    SciTech Connect (OSTI)

    Ren, Yan; Perepelov, Andrei V.; Wang, Haiyan; Zhang, Hao; Knirel, Yuriy A.; Wang, Lei; Chen, Wei

    2010-01-22

    Mortierella alpina is a filamentous fungus commonly found in soil, which is able to produce large amount of polyunsaturated fatty acids. L-Fucose is an important sugar found in a diverse range of organisms, playing a variety of biological roles. In this study, we characterized the de novo biosynthetic pathway of GDP-L-fucose (the nucleotide-activated form of L-fucose) in M. alpina. Genes encoding GDP-D-mannose 4,6-dehydratase (GMD) and GDP-keto-6-deoxymannose 3,5-epimerase/4-reductase (GMER) were expressed heterologously in Escherichia coli. The recombinant enzymes were produced as His-tagged fusion proteins. Conversion of GDP-mannose to GDP-4-keto-6-deoxy mannose by GMD and GDP-4-keto-6-deoxy mannose to GDP-L-fucose by GMER were analyzed by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy. The k{sub m} values of GMD for GDP-mannose and GMER for GDP-4-keto-6-deoxy mannose were determined to be 0.77 mM and 1.047 mM, respectively. Both NADH and NADPH may be used by GMER as the coenzyme. The optimum temperature and pH were determined to be 37 {sup o}C and pH 9.0 (GMD) or pH 7.0 (GMER). Divalent cations are not required for GMD and GMER activity, and the activities of both enzymes may be enhanced by DTT. To our knowledge this is the first report on the characterization of GDP-L-fucose biosynthetic pathway in fungi.

  20. Total Natural Gas Gross Withdrawals (Summary)

    U.S. Energy Information Administration (EIA) Indexed Site

    Pipeline and Distribution Use Price Citygate Price Residential Price Commercial Price Industrial Price Vehicle Fuel Price Electric Power Price Proved Reserves as of 12/31 Reserves Adjustments Reserves Revision Increases Reserves Revision Decreases Reserves Sales Reserves Acquisitions Reserves Extensions Reserves New Field Discoveries New Reservoir Discoveries in Old Fields Estimated Production Number of Producing Gas Wells Gross Withdrawals Gross Withdrawals From Gas Wells Gross Withdrawals From

  1. ,"Texas Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Texas Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  2. ,"Kansas Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Kansas Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  3. ,"Pennsylvania Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Pennsylvania Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  4. ,"Kentucky Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Kentucky Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  5. ,"Oregon Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Oregon Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301979" ,"Release...

  6. ,"Virginia Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Virginia Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  7. ,"Missouri Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Missouri Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  8. ,"Illinois Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Illinois Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  9. ,"Florida Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Florida Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  10. ,"Utah Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Utah Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  11. ,"Indiana Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Indiana Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  12. ,"Nevada Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Nevada Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301991" ,"Release...

  13. ,"Montana Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Montana Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  14. ,"Ohio Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Ohio Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  15. ,"California Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","California Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  16. ,"Mississippi Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Mississippi Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  17. ,"Nebraska Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Nebraska Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  18. ,"Michigan Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Michigan Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  19. ,"Tennessee Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Tennessee Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  20. ,"Oklahoma Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Oklahoma Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  1. ,"Wyoming Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Wyoming Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  2. ,"Maryland Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Maryland Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  3. ,"Louisiana Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Louisiana Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  4. ,"Colorado Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Colorado Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  5. David J. Gross and the Strong Force

    Office of Scientific and Technical Information (OSTI)

    from Cal Alum David Gross (PhD '66) Shares Nobel Prize in Physics, University of California Berkeley Resources with Additional Information Additional information about David ...

  6. ,"Illinois Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...ame","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Illinois Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151991" ,"Release ...

  7. ,"Oregon Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Oregon Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151991" ,"Release ...

  8. ,"California Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Of Series","Frequency","Latest Data for" ,"Data 1","California Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  9. ,"Louisiana Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Of Series","Frequency","Latest Data for" ,"Data 1","Louisiana Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  10. ,"Oklahoma Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...ame","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Oklahoma Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  11. ,"Pennsylvania Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Of Series","Frequency","Latest Data for" ,"Data 1","Pennsylvania Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151991" ,"Release ...

  12. ,"Colorado Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...ame","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Colorado Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  13. ,"Florida Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Florida Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  14. ,"Utah Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Utah Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  15. ,"Montana Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Montana Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  16. ,"Kansas Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Kansas Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  17. ,"Texas Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Texas Natural Gas Gross Withdrawals and Production",10,"Monthly","42016","01151989" ,"Release ...

  18. David J. Gross and the Strong Force

    Office of Scientific and Technical Information (OSTI)

    published their proposal simultaneously with H. David Politzer, a graduate student at Harvard University who independently came up with the same idea. ... The discovery of Gross,...

  19. ,"Wyoming Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Wyoming Natural Gas Gross Withdrawals and Production",10,"Monthly","32016","01151989" ,"Release ...

  20. Unions, LANL sign labor agreements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unions, LANL sign labor agreements Unions, LANL sign labor agreements The New Mexico Building and Construction Trades Council and Los Alamos National Security LLC, announced the signing of a series of labor agreements that affect nearly 750 construction and maintenance workers. September 21, 2012 Jay Johnson renews the contracts with the Laboratory's union workers. Jay Johnson (left) associate director for maintenance and infrastructure planning, renews the contracts with the Laboratory's union

  1. Crystal structure of a tetrameric GDP-D-mannose 4,6-dehydratase from a bacterial GDP-D-rhamnose biosynthetic pathway

    SciTech Connect (OSTI)

    Webb, N.A.; Mulichak, A.M.; Lam, J.S.; Rocchetta, H.L.; Garavito, R.M.

    2010-03-08

    D-Rhamnose is a rare 6-deoxy monosaccharide primarily found in the lipopolysaccharide of pathogenic bacteria, where it is involved in host-bacterium interactions and the establishment of infection. The biosynthesis of D-rhamnose proceeds through the conversion of GDP-D-mannose by GDP-D-mannose 4,6-dehydratase (GMD) to GDP-4-keto-6-deoxymannose, which is subsequently reduced to GDP-D-rhamnose by a reductase. We have determined the crystal structure of GMD from Pseudomonas aeruginosa in complex with NADPH and GDP. GMD belongs to the NDP-sugar modifying subfamily of the short-chain dehydrogenase/reductase (SDR) enzymes, all of which exhibit bidomain structures and a conserved catalytic triad (Tyr-XXX-Lys and Ser/Thr). Although most members of this enzyme subfamily display homodimeric structures, this bacterial GMD forms a tetramer in the same fashion as the plant MUR1 from Arabidopsis thaliana. The cofactor binding sites are adjoined across the tetramer interface, which brings the adenosyl phosphate moieties of the adjacent NADPH molecules to within 7 {angstrom} of each other. A short peptide segment (Arg35-Arg43) stretches into the neighboring monomer, making not only protein-protein interactions but also hydrogen bonding interactions with the neighboring cofactor. The interface hydrogen bonds made by the Arg35-Arg43 segment are generally conserved in GMD and MUR1, and the interacting residues are highly conserved among the sequences of bacterial and eukaryotic GMDs. Outside of the Arg35-Arg43 segment, residues involved in tetrameric contacts are also quite conserved across different species. These observations suggest that a tetramer is the preferred, and perhaps functionally relevant, oligomeric state for most bacterial and eukaryotic GMDs.

  2. ,"Arizona Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,"(202) 586-8800",,,"06292016 10:51:24 AM" "Back to Contents","Data 1: Arizona Natural Gas Gross Withdrawals and Production" "Sourcekey","N9010AZ2","N9011AZ2","N9012AZ2","NGME...

  3. ,"Arkansas Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,"(202) 586-8800",,,"06292016 10:51:23 AM" "Back to Contents","Data 1: Arkansas Natural Gas Gross Withdrawals and Production" "Sourcekey","N9010AR2","N9011AR2","N9012AR2","NGME...

  4. ,"Arizona Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,"(202) 586-8800",,,"06292016 10:51:23 AM" "Back to Contents","Data 1: Arizona Natural Gas Gross Withdrawals and Production" "Sourcekey","N9010AZ2","N9011AZ2","N9012AZ2","NGME...

  5. ,"Alabama Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,"(202) 586-8800",,,"06292016 10:51:21 AM" "Back to Contents","Data 1: Alabama Natural Gas Gross Withdrawals and Production" "Sourcekey","N9010AL2","N9011AL2","N9012AL2","NGME...

  6. ,"New York Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,"(202) 586-8800",,,"12152015 12:10:48 PM" "Back to Contents","Data 1: New York Natural Gas Gross Withdrawals (MMcf)" "Sourcekey","N9010NY2" "Date","New York...

  7. ,"Alaska Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,"(202) 586-8800",,,"4292016 6:48:19 AM" "Back to Contents","Data 1: Alaska Natural Gas Gross Withdrawals and Production" "Sourcekey","N9010AK2","N9011AK2","N9012AK2","NGME...

  8. Quantification of the Potential Gross Economic Impacts of Five...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Quantification of the Potential Gross Economic Impacts of Five Methane Reduction Scenarios Quantification of the Potential Gross Economic Impacts of Five Methane Reduction Scenarios ...

  9. ,"Texas Natural Gas Gross Withdrawals Total Offshore (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Texas Natural Gas Gross Withdrawals Total ... 7:03:02 AM" "Back to Contents","Data 1: Texas Natural Gas Gross Withdrawals Total ...

  10. Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals...

    U.S. Energy Information Administration (EIA) Indexed Site

    Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Natural Gas Gross Withdrawals from Shale Gas Wells Federal Offshore Gulf of Mexico Natural Gas Gross ...

  11. ,"US--Federal Offshore Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    Federal Offshore Natural Gas Gross Withdrawals (MMcf)" ,"Click worksheet name or tab at ... Data for" ,"Data 1","US--Federal Offshore Natural Gas Gross Withdrawals ...

  12. ,"Texas--State Offshore Natural Gas Gross Withdrawals (MMcf)...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Texas--State Offshore Natural Gas Gross Withdrawals ... "Back to Contents","Data 1: Texas--State Offshore Natural Gas Gross Withdrawals (MMcf)" ...

  13. ,"Federal Offshore--Texas Natural Gas Gross Withdrawals (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Federal Offshore--Texas Natural Gas Gross Withdrawals ... AM" "Back to Contents","Data 1: Federal Offshore--Texas Natural Gas Gross Withdrawals ...

  14. ,"US--State Offshore Natural Gas Gross Withdrawals (MMcf)"

    U.S. Energy Information Administration (EIA) Indexed Site

    State Offshore Natural Gas Gross Withdrawals (MMcf)" ,"Click worksheet name or tab at ... Data for" ,"Data 1","US--State Offshore Natural Gas Gross Withdrawals ...

  15. ,"Federal Offshore--Alabama Natural Gas Gross Withdrawals (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Federal Offshore--Alabama Natural Gas Gross Withdrawals ... AM" "Back to Contents","Data 1: Federal Offshore--Alabama Natural Gas Gross Withdrawals ...

  16. ,"Louisiana--State Offshore Natural Gas Gross Withdrawals (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Louisiana--State Offshore Natural Gas Gross Withdrawals ... to Contents","Data 1: Louisiana--State Offshore Natural Gas Gross Withdrawals (MMcf)" ...

  17. ,"Alaska--State Offshore Natural Gas Gross Withdrawals (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Alaska--State Offshore Natural Gas Gross Withdrawals ... to Contents","Data 1: Alaska--State Offshore Natural Gas Gross Withdrawals (MMcf)" ...

  18. Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals...

    U.S. Energy Information Administration (EIA) Indexed Site

    Release Date: 06302016 Next Release Date: 07292016 Referring Pages: Natural Gas Gross Withdrawals from Coalbed Wells Federal Offshore Gulf of Mexico Natural Gas Gross ...

  19. ,"Federal Offshore--Louisiana Natural Gas Gross Withdrawals ...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Federal Offshore--Louisiana Natural Gas Gross Withdrawals ... AM" "Back to Contents","Data 1: Federal Offshore--Louisiana Natural Gas Gross Withdrawals ...

  20. ,"Alabama--State Offshore Natural Gas Gross Withdrawals (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Alabama--State Offshore Natural Gas Gross Withdrawals ... to Contents","Data 1: Alabama--State Offshore Natural Gas Gross Withdrawals (MMcf)" ...

  1. ,"Federal Offshore Gulf of Mexico Natural Gas Gross Withdrawals...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gulf of Mexico Natural Gas Gross Withdrawals and Production" ,"Click worksheet name or tab ... for" ,"Data 1","Federal Offshore Gulf of Mexico Natural Gas Gross Withdrawals and ...

  2. Property:DailyOpWaterUseGross | Open Energy Information

    Open Energy Info (EERE)

    Property Name DailyOpWaterUseGross Property Type Number Description Daily Operation Water Use (afday) Gross. Retrieved from "http:en.openei.orgwindex.php?titleProperty:...

  3. Table 2. Real Gross Domestic Product Growth Trends, Projected vs. Actual

    U.S. Energy Information Administration (EIA) Indexed Site

    Real Gross Domestic Product Growth Trends, Projected vs. Actual Projected Real GDP Growth Trend (cumulative average percent growth in projected real GDP from first year shown for each AEO) 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 AEO 1994 3.09 3.15 2.86 2.78 2.73 2.65 2.62 2.60 2.56 2.53 2.52 2.49 2.45 2.41 2.40 2.36 2.32 2.29 AEO 1995 3.66 2.77 2.53 2.71 2.67 2.61 2.55 2.48 2.46 2.45 2.45 2.43 2.39 2.35 2.31 2.27 2.24 AEO 1996 2.61

  4. Unions, LANL sign labor agreements

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the contracts with the Laboratory's union workers. To the right of Johnson are Ray Baca of the New Mexico Building and Construction Trades Council and Tim Babicke and Gerard...

  5. A Rac1--GDP trimer complex binds zinc with tetrahedral and octahedral coordination, displacing magnesium

    SciTech Connect (OSTI)

    Prehna, G.; Stebbins, C

    2007-01-01

    The Rho family of small GTPases represent well characterized signaling molecules that regulate many cellular functions such as actin cytoskeletal arrangement and the cell cycle by acting as molecular switches. A Rac1-GDP-Zn complex has been crystallized in space group P3221 and its crystal structure has been solved at 1.9 {angstrom} resolution. These trigonal crystals reveal the unexpected ability of Rac1 to coordinate Zn atoms in a tetrahedral fashion by use of its biologically relevant switch I and switch II regions. Upon coordination of zinc, the switch I region is stabilized in the GDP-bound conformation and contributes to a Rac1 trimer in the asymmetric unit. Zinc coordination causes switch II to adopt a novel conformation with a symmetry-related molecule. Additionally, zinc was found to displace magnesium from its octahedral coordination at switch I, although GDP binding remained stable. This structure represents the first reported Rac1-GDP-Zn complex, which further underscores the conformational flexibility and versatility of the small GTPase switch regions.

  6. A Rac1-GDP Trimer Complex Binds Zinc with Tetrahedral and Octahedral Coordination, Displacing Magnesium

    SciTech Connect (OSTI)

    Prehna,G.; Stebbins, E.

    2007-01-01

    The Rho family of small GTPases represent well characterized signaling molecules that regulate many cellular functions such as actin cytoskeletal arrangement and the cell cycle by acting as molecular switches. A Rac1-GDP-Zn complex has been crystallized in space group P3{sub 2}21 and its crystal structure has been solved at 1.9 {angstrom} resolution. These trigonal crystals reveal the unexpected ability of Rac1 to coordinate Zn atoms in a tetrahedral fashion by use of its biologically relevant switch I and switch II regions. Upon coordination of zinc, the switch I region is stabilized in the GDP-bound conformation and contributes to a Rac1 trimer in the asymmetric unit. Zinc coordination causes switch II to adopt a novel conformation with a symmetry-related molecule. Additionally, zinc was found to displace magnesium from its octahedral coordination at switch I, although GDP binding remained stable. This structure represents the first reported Rac1-GDP-Zn complex, which further underscores the conformational flexibility and versatility of the small GTPase switch regions.

  7. Oregon Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    2012 2013 2014 View History Gross Withdrawals 821 1,407 1,344 770 770 950 1979-2014 From Gas Wells 821 1,407 1,344 770 770 950 1979-2014 From Oil Wells 0 0 0 0 0 0 1996-2014 From ...

  8. FORT UNION DEEP

    SciTech Connect (OSTI)

    Lyle A. Johnson Jr.

    2002-03-01

    Coalbed methane (CBM) is currently the hottest area of energy development in the Rocky Mountain area. The Powder River Basin (PRB) is the largest CBM area in Wyoming and has attracted the majority of the attention because of its high permeability and relatively shallow depth. Other Wyoming coal regions are also being targeted for development, but most of these areas have lower permeability and deeper coal seams. This project consists of the development of a CBM stimulation system for deep coal resources and involves three work areas: (1) Well Placement, (2) Well Stimulation, and (3) Production Monitoring and Evaluation. The focus of this project is the Washakie Basin. Timberline Energy, Inc., the cosponsor, has a project area in southern Carbon County, Wyoming, and northern Moffat County, Colorado. The target coal is found near the top of the lower Fort Union formation. The well for this project, Evans No.1, was drilled to a depth of 2,700 ft. Three coal seams were encountered with sandstone and some interbedded shale between seams. Well logs indicated that the coal seams and the sandstone contained gas. For the testing, the upper seam at 2,000 ft was selected. The well, drilled and completed for this project, produced very little water and only occasional burps of methane. To enhance the well, a mild severity fracture was conducted to fracture the coal seam and not the adjacent sandstone. Fracturing data indicated a fracture half-length of 34 ft, a coal permeability of 0.2226 md, and permeability of 15.3 md. Following fracturing, the gas production rate stabilized at 10 Mscf/day within water production of 18 bpd. The Western Research Institute (WRI) CBM model was used to design a 14-day stimulation cycle followed by a 30-day production period. A maximum injection pressure of 1,200 psig to remain well below the fracture pressure was selected. Model predictions were 20 Mscf/day of air injection for 14 days, a one-day shut-in, then flowback. The predicted flowback

  9. FORT UNION DEEP

    SciTech Connect (OSTI)

    Lyle A. Johnson Jr.

    2002-09-01

    Coalbed methane (CBM) is currently the hottest area of energy development in the Rocky Mountain area. The Powder River Basin (PRB) is the largest CBM area in Wyoming and has attracted the majority of the attention because of its high permeability and relatively shallow depth. Other Wyoming coal regions are also being targeted for development, but most of these areas have lower permeability and deeper coal seams. This project consists of the development of a CBM stimulation system for deep coal resources and involves three work areas: (1) Well Placement, (2) Well Stimulation, and (3) Production Monitoring and Evaluation. The focus of this project is the Washakie Basin. Timberline Energy, Inc., the cosponsor, has a project area in southern Carbon County, Wyoming, and northern Moffat County, Colorado. The target coal is found near the top of the lower Fort Union formation. The well for this project, Evans No.1, was drilled to a depth of 2,700 ft. Three coal seams were encountered with sandstone and some interbedded shale between seams. Well logs indicated that the coal seams and the sandstone contained gas. For the testing, the upper seam at 2,000 ft was selected. The well, drilled and completed for this project, produced very little water and only occasional burps of methane. To enhance the well, a mild severity fracture was conducted to fracture the coal seam and not the adjacent sandstone. Fracturing data indicated a fracture half-length of 34 ft, a coal permeability of 0.2226 md, and permeability of 15.3 md. Following fracturing, the gas production rate stabilized at 10 Mscf/day within water production of 18 bpd. The Western Research Institute (WRI) CBM model was used to design a 14-day stimulation cycle followed by a 30-day production period. A maximum injection pressure of 1,200 psig to remain well below the fracture pressure was selected. Model predictions were 20 Mscf/day of air injection for 14 days, a one-day shut-in, then flowback. The predicted flowback

  10. Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals...

    Gasoline and Diesel Fuel Update (EIA)

    Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals (Million Cubic Feet per...

  11. ,"Kansas Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Kansas Natural Gas Gross Withdrawals from Shale ... 7:12:26 AM" "Back to Contents","Data 1: Kansas Natural Gas Gross Withdrawals from Shale ...

  12. ,"Nevada Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Nevada Natural Gas Gross Withdrawals from Shale ... 1:29:33 AM" "Back to Contents","Data 1: Nevada Natural Gas Gross Withdrawals from Shale ...

  13. ,"U.S. Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    6:48:07 AM" "Back to Contents","Data 1: U.S. Natural Gas Gross Withdrawals and ...2","N9030US2","N9050US2","N9070US2" "Date","U.S. Natural Gas Gross Withdrawals ...

  14. ,"U.S. Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    1:00:33 PM" "Back to Contents","Data 1: U.S. Natural Gas Gross Withdrawals and ...2","N9030US2","N9050US2","N9070US2" "Date","U.S. Natural Gas Gross Withdrawals ...

  15. West Virginia Natural Gas Gross Withdrawals (Million Cubic Feet...

    Gasoline and Diesel Fuel Update (EIA)

    Gross Withdrawals (Million Cubic Feet per Day) West Virginia Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006...

  16. Montana Natural Gas Gross Withdrawals (Million Cubic Feet per...

    Gasoline and Diesel Fuel Update (EIA)

    Gross Withdrawals (Million Cubic Feet per Day) Montana Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006 317 313...

  17. California Natural Gas Gross Withdrawals (Million Cubic Feet...

    Gasoline and Diesel Fuel Update (EIA)

    Gross Withdrawals (Million Cubic Feet per Day) California Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006 998...

  18. Missouri Natural Gas Gross Withdrawals from Oil Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Oil Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 0 ... Referring Pages: Natural Gas Gross Withdrawals from Oil Wells Missouri Natural Gas Gross ...

  19. Virginia Natural Gas Gross Withdrawals (Million Cubic Feet per...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Gross Withdrawals (Million Cubic Feet per Day) Virginia Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006 271 275...

  20. ,"Texas Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","Texas Natural Gas Gross Withdrawals from Shale ... 7:12:29 AM" "Back to Contents","Data 1: Texas Natural Gas Gross Withdrawals from Shale ...

  1. New York Natural Gas Gross Withdrawals (Million Cubic Feet per...

    Gasoline and Diesel Fuel Update (EIA)

    Gross Withdrawals (Million Cubic Feet per Day) New York Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006 149 147...

  2. ,"New Mexico Natural Gas Gross Withdrawals from Oil Wells (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals from ... 10:10:49 AM" "Back to Contents","Data 1: New Mexico Natural Gas Gross Withdrawals from ...

  3. ,"New Mexico Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals from ... 10:13:23 AM" "Back to Contents","Data 1: New Mexico Natural Gas Gross Withdrawals from ...

  4. ,"New Mexico Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    ...","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals and ... 8:15:20 AM" "Back to Contents","Data 1: New Mexico Natural Gas Gross Withdrawals and ...

  5. ,"New Mexico Natural Gas Gross Withdrawals from Gas Wells (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals from ... 10:10:30 AM" "Back to Contents","Data 1: New Mexico Natural Gas Gross Withdrawals from ...

  6. ,"New York Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    ...","Frequency","Latest Data for" ,"Data 1","New York Natural Gas Gross Withdrawals from ... 10:13:24 AM" "Back to Contents","Data 1: New York Natural Gas Gross Withdrawals from ...

  7. Kansas Natural Gas Gross Withdrawals (Million Cubic Feet per...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Gross Withdrawals (Million Cubic Feet per Day) Kansas Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006 1,049...

  8. Arizona Natural Gas Gross Withdrawals (Million Cubic Feet per...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Arizona Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Arizona Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct...

  9. ,"North Dakota Natural Gas Gross Withdrawals from Oil Wells ...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","North Dakota Natural Gas Gross Withdrawals from ... 9:52:34 AM" "Back to Contents","Data 1: North Dakota Natural Gas Gross Withdrawals from ...

  10. ,"North Dakota Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","North Dakota Natural Gas Gross Withdrawals and ... 10:51:41 AM" "Back to Contents","Data 1: North Dakota Natural Gas Gross Withdrawals and ...

  11. ,"North Dakota Natural Gas Gross Withdrawals from Gas Wells ...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","North Dakota Natural Gas Gross Withdrawals from ... 9:52:18 AM" "Back to Contents","Data 1: North Dakota Natural Gas Gross Withdrawals from ...

  12. ,"North Dakota Natural Gas Gross Withdrawals from Shale Gas ...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data for" ,"Data 1","North Dakota Natural Gas Gross Withdrawals from ... 9:55:03 AM" "Back to Contents","Data 1: North Dakota Natural Gas Gross Withdrawals from ...

  13. New Mexico Natural Gas Gross Withdrawals (Million Cubic Feet...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Gross Withdrawals (Million Cubic Feet per Day) New Mexico Natural Gas Gross Withdrawals (Million Cubic Feet per Day) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2006 4,406...

  14. Cloning, purification and preliminary crystallographic analysis of the Bacillus subtilis GTPase YphC–GDP complex

    SciTech Connect (OSTI)

    Xu, Ling; Muench, Stephen P.; Roujeinikova, Anna; Sedelnikova, Svetlana E.; Rice, David W.

    2006-05-01

    Crystals of a selenomethionine-incorporated YphC–GDP complex have been grown using the hanging-drop vapour-diffusion method and polyethylene glycol as a precipitating agent. The Bacillus subtilis YphC gene encodes an essential GTPase thought to be involved in ribosome binding and whose protein product may represent a target for the development of a novel antibacterial agent. Sequence analysis reveals that YphC belongs to the EngA family of GTPases, which uniquely contain two adjacent GTP-binding domains. Crystals of a selenomethionine-incorporated YphC–GDP complex have been grown using the hanging-drop vapour-diffusion method and polyethylene glycol as a precipitating agent. The crystals belong to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 62.71, b = 65.05, c = 110.61 Ĺ, and have one molecule in the asymmetric unit. Data sets at three different wavelengths were collected on a single crystal to 2.5 Ĺ resolution at the Daresbury SRS in order to solve the structure by MAD. Ultimately, analysis of YphC in complex with GDP may allow a greater understanding of the EngA family of essential GTPases.

  15. Characterization of a Ras Mutant with Identical GDP- and GTP-Bound Structures

    SciTech Connect (OSTI)

    Ford, B.; Boykevisch, S; Zhao, C; Kunzelmann, S; Bar-Sagi, D; Herrmann, C; Nassar, N

    2009-01-01

    We previously characterized the G60A mutant of Ras and showed that the switch regions of the GTP-bound but not the GDP-bound form of this mutant adopt an 'open conformation' similar to that seen in nucleotide-free Ras. Here, we mutate Lys147 of the conserved {sup 145}SAK{sup 147} motif in the G60A background and characterize the resulting double mutant (DM). We show that RasDM is the first structure of a Ras protein with identical GDP- and GTP-bound structures. Both structures adopt the open conformation of the active form of RasG60A. The increase in the accessible surface area of the nucleotide is consistent with a 4-fold increase in its dissociation rate. Stopped-flow experiments show no major difference in the two-step kinetics of association of GDP or GTP with the wild type, G60A, or RasDM. Addition of Sos fails to accelerate nucleotide exchange. Overexpression of the G60A or double mutant of Ras in COS-1 cells fails to activate Erk and shows a strong dominant negative effect. Our data suggest that flexibility at position 60 is required for proper Sos-catalyzed nucleotide exchange and that structural information is somehow shared among the switch regions and the different nucleotide binding motifs.

  16. International Union for Conservation of Nature | Open Energy...

    Open Energy Info (EERE)

    Union for Conservation of Nature Jump to: navigation, search Logo: International Union for Conservation of Nature Name: International Union for Conservation of Nature Address: Rue...

  17. Agro Unione Ltda | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: Agro Unione Ltda Place: Brazil Product: Sugar and ethanol producer. References: Agro Unione Ltda1 This article is a stub. You can help OpenEI...

  18. Virginia Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    09 2010 2011 2012 2013 2014 View History Gross Withdrawals 140,738 147,255 151,094 146,405 139,382 131,885 1967-2014 From Gas Wells 16,046 23,086 20,375 21,802 26,815 27,052 1967-2014 From Oil Wells 0 0 0 9 9 9 2006-2014 From Shale Gas Wells 18,284 16,433 18,501 17,212 13,016 12,226 2007-2014 From Coalbed Wells 106,408 107,736 112,219 107,383 99,542 92,599 2006-2014 Repressuring 0 0 0 0 0 0 2003-2014 Vented and Flared NA NA NA 0 NA NA 1967-2014 Nonhydrocarbon Gases Removed 0 0 0 0 0 0 1997-2014

  19. Kansas Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    10 2011 2012 2013 2014 2015 View History Gross Withdrawals 325,591 309,952 296,299 292,467 286,080 292,450 1967-2015 From Gas Wells 247,651 236,834 264,610 264,223 260,715 1967-2014 From Oil Wells 39,071 37,194 0 0 0 1967-2014 From Shale Gas Wells 0 0 0 0 0 2007-2014 From Coalbed Wells 38,869 35,924 31,689 28,244 25,365 2002-2014 Repressuring 548 521 0 NA NA 1967-2014 Vented and Flared 323 307 0 NA NA 1967-2014 Nonhydrocarbon Gases Removed 0 0 0 0 0 2002-2014 Marketed Production 324,720 309,124

  20. Kentucky Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    09 2010 2011 2012 2013 2014 View History Gross Withdrawals 113,300 135,330 124,243 106,122 94,665 78,737 1967-2014 From Gas Wells 111,782 133,521 122,578 106,122 94,665 78,737 1967-2014 From Oil Wells 1,518 1,809 1,665 0 0 0 1967-2014 From Shale Gas Wells 0 0 0 0 0 0 2007-2014 From Coalbed Wells 0 0 0 0 0 0 2006-2014 Repressuring 0 0 0 0 0 0 2006-2014 Vented and Flared 0 0 0 0 0 0 1967-2014 Nonhydrocarbon Gases Removed 0 0 0 0 0 0 2006-2014 Marketed Production 113,300 135,330 124,243 106,122

  1. Louisiana Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    10 2011 2012 2013 2014 2015 View History Gross Withdrawals 2,218,283 3,040,523 2,955,437 2,366,943 1,987,630 1,941,727 1967-2015 From Gas Wells 911,967 883,712 775,506 780,623 737,185 1967-2014 From Oil Wells 63,638 68,505 49,380 51,948 50,638 1967-2014 From Shale Gas Wells 1,242,678 2,088,306 2,130,551 1,534,372 1,199,807 2007-2014 From Coalbed Wells 0 0 0 0 0 2002-2014 Repressuring 3,606 5,015 0 2,829 3,199 1967-2014 Vented and Flared 4,578 6,302 0 3,912 4,143 1967-2014 Nonhydrocarbon Gases

  2. Nebraska Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    09 2010 2011 2012 2013 2014 View History Gross Withdrawals 2,916 2,255 1,980 1,328 1,032 402 1967-2014 From Gas Wells 2,734 2,092 1,854 1,317 1,027 400 1967-2014 From Oil Wells 182 163 126 11 5 1 1967-2014 From Shale Gas Wells 0 0 0 0 0 0 2007-2014 From Coalbed Wells 0 0 0 0 0 0 2006-2014 Repressuring 0 0 0 0 0 0 1967-2014 Vented and Flared 9 24 21 0 NA NA 1967-2014 Nonhydrocarbon Gases Removed 0 0 0 0 0 0 2006-2014 Marketed Production 2,908 2,231 1,959 1,328 1,032 402 1967-2014 Dry Production

  3. Pennsylvania Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    10 2011 2012 2013 2014 2015 View History Gross Withdrawals 572,902 1,310,592 2,256,696 3,259,042 4,214,643 4,768,848 1967-2015 From Gas Wells 173,450 242,305 210,609 207,872 174,576 1967-2014 From Oil Wells 0 0 3,456 2,987 3,564 1967-2014 From Shale Gas Wells 399,452 1,068,288 2,042,632 3,048,182 4,036,504 2007-2014 From Coalbed Wells 0 0 0 0 0 2006-2014 Repressuring 0 0 0 0 0 1967-2014 Vented and Flared 0 0 0 0 0 1967-2014 Nonhydrocarbon Gases Removed 0 0 0 0 0 1997-2014 Marketed Production

  4. ,"New Mexico Natural Gas Gross Withdrawals from Oil Wells (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals from Oil Wells (MMcf)",1,"Annual",2014 ,"Release...

  5. ,"New Mexico Natural Gas Gross Withdrawals from Gas Wells (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals from Gas Wells (MMcf)",1,"Annual",2014 ,"Release...

  6. ,"Federal Offshore Gulf of Mexico Natural Gas Gross Withdrawals...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gulf of Mexico Natural Gas Gross Withdrawals and Production" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description"," Of Series","Frequency","Latest...

  7. ,"New Mexico Natural Gas Gross Withdrawals and Production"

    U.S. Energy Information Administration (EIA) Indexed Site

    Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","New Mexico Natural Gas Gross Withdrawals and Production",10,"Annual",2014,"06301967" ,"Release...

  8. ,"West Virginia Natural Gas Gross Withdrawals from Shale Gas...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  9. ,"Tennessee Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  10. ,"Missouri Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  11. ,"Louisiana Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  12. ,"Wyoming Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  13. ,"Michigan Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  14. ,"Mississippi Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  15. ,"Virginia Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  16. ,"Oregon Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  17. ,"Oklahoma Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  18. ,"Utah Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  19. ,"Ohio Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  20. ,"Montana Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  1. ,"South Dakota Natural Gas Gross Withdrawals from Shale Gas ...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet)" ,"Click worksheet name or tab at bottom for data" ... Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet)",1,"Monthly","1...

  2. Gross Gamma-Ray Calibration Blocks (May 1978) | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    for, and the Procedures Currently Utilized in, Gross Gamma-Ray Log Calibration (October 1976) Parameter Assignments for Spectral Gamma-Ray Borehole Calibration Models (April 1984

  3. ,"West Virginia Natural Gas Gross Withdrawals and Production...

    U.S. Energy Information Administration (EIA) Indexed Site

    Of Series","Frequency","Latest Data for" ,"Data 1","West Virginia Natural Gas Gross Withdrawals and Production",10,"Monthly","22016","1151991" ,"Release ...

  4. ,"Other States Total Natural Gas Gross Withdrawals and Production...

    U.S. Energy Information Administration (EIA) Indexed Site

    Total Natural Gas Gross Withdrawals and Production" ,"Click worksheet name or tab at bottom for data" ,"Worksheet Name","Description"," Of Series","Frequency","Latest Data for" ...

  5. Union Eolica Panamena UEP | Open Energy Information

    Open Energy Info (EERE)

    Eolica Panamena UEP Jump to: navigation, search Name: Union Eolica Panamena (UEP) Place: Granada, Spain Sector: Wind energy Product: Granada-based wind project developer company,...

  6. GC GUIDANCE ON MEETINGS WITH UNION OFFICIALS

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    matter, the program official should obtain the individual's name, the contract and contractor of interest, the identity of the union and the subject of the requested meeting. ...

  7. Structural basis of GDP release and gating in G protein coupled Fe[superscript 2+] transport

    SciTech Connect (OSTI)

    Guilfoyle, Amy; Maher, Megan J.; Rapp, Mikaela; Clarke, Ronald; Harrop, Stephen; Jormakka, Mika

    2009-09-29

    G proteins are key molecular switches in the regulation of membrane protein function and signal transduction. The prokaryotic membrane protein FeoB is involved in G protein coupled Fe{sup 2+} transport, and is unique in that the G protein is directly tethered to the membrane domain. Here, we report the structure of the soluble domain of FeoB, including the G protein domain, and its assembly into an unexpected trimer. Comparisons between nucleotide free and liganded structures reveal the closed and open state of a central cytoplasmic pore, respectively. In addition, these data provide the first observation of a conformational switch in the nucleotide-binding G5 motif, defining the structural basis for GDP release. From these results, structural parallels are drawn to eukaryotic G protein coupled membrane processes.

  8. June 11, 2009, HSS/Union Task Meeting on 2009 HSS/Union Task Progress - Agenda

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    06-05-09 HSS/Union Working Group Meeting June 11, 2009 1:00 - 2:30 pm EST FORS 7E-069 Call-in: 301-903-0688 SUBJECT: 2009 HSS/Union Task Progress Union Working Group Participants: 2008 Topical Union Leads Ron Ault/Tom Schaffer..................AFL-CIO Metal Trades Department Pete Stafford...................................... Building and Construction Trades Department Center for Construction Research & Training (BCTD CPWR) Chico McGill/Dennis Phelps............International Brotherhood of

  9. June 11, 2009, HSS/Union Task Meeting on 2009 HSS/Union Task Progress - Summary

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    23-09 HSS/Union Working Group Meeting 2009 HSS/Union Task Progress and Next Steps June 11, 2009 In introductory remarks, Glenn Podonsky, Chief Health, Safety and Security Officer, underscored HSS's commitment to engage in this collective effort with the Unions and stakeholders to improve worker health, safety and security across the DOE Complex. He provided an overview of the past several years of the HSS Focus Group effort in which the worker, through Union representatives and stakeholders,

  10. RĂ©union publique HR

    ScienceCinema (OSTI)

    None

    2011-10-06

    Chers Collègues,Je me permets de vous rappeler qu'une réunion publique organisée par le Département HR se tiendra aujourd'hui:Vendredi 30 avril 2010 à 9h30 dans l'Amphithéâtre principal (café offert dès 9h00).Durant cette réunion, des informations générales seront données sur:le CERN Admin e-guide, qui est un nouveau guide des procédures administratives du CERN ayant pour but de faciliter la recherche d'informations pratiques et d'offrir un format de lecture convivial;le régime d'Assurance Maladie de l'Organisation (présentation effectuée par Philippe Charpentier, Président du CHIS Board) et;la Caisse de Pensions (présentation effectuée par Théodore Economou, Administrateur de la Caisse de Pensions du CERN).Une transmission simultanée de cette réunion sera assurée dans l'Amphithéâtre BE de Prévessin et également disponible à l'adresse suivante: http://webcast.cern.chJe me réjouis de votre participation!Meilleures salutations,Anne-Sylvie CatherinChef du Département des Ressources humaines__________________________________________________________________________________Dear Colleagues,I should like to remind you that a plublic meeting organised by HR Department will be held today:Friday 30 April 2010 at 9:30 am in the Main Auditorium (coffee from 9:00 am).During this meeting, general information will be given about:the CERN Admin e-guide which is a new guide to the Organization's administrative procedures, drawn up to facilitate the retrieval of practical information and to offer a user-friendly format;the CERN Health Insurance System (presentation by Philippe Charpentier, President of the CHIS Board) and;the Pension Fund (presentation by Theodore Economou, Administrator of the CERN Pension Fund).A simultaneous transmission of this meeting will be broadcast in the BE Auditorium at Prévessin and will also be available at the following address. http://webcast.cern.chI look forward to your participation!Best regards,Anne-Sylvie Catherin

  11. South Dakota Natural Gas Gross Withdrawals from Coalbed Wells (Million

    U.S. Energy Information Administration (EIA) Indexed Site

    Cubic Feet) Coalbed Wells (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 2000's 0 0 0 0 2010's 0 0 0 0 0 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 8/31/2016 Next Release Date: 9/30/2016 Referring Pages: Natural Gas Gross Withdrawals from Coalbed Wells South Dakota Natural Gas Gross Withdrawals and Production Natural Gas Gross Withdrawals from

  12. Other States Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Other States Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 72,328 ...

  13. Other States Natural Gas Gross Withdrawals from Coalbed Wells...

    U.S. Energy Information Administration (EIA) Indexed Site

    Coalbed Wells (Million Cubic Feet) Other States Natural Gas Gross Withdrawals from Coalbed Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2002 0 0 ...

  14. Other States Natural Gas Gross Withdrawals from Oil Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Oil Wells (Million Cubic Feet) Other States Natural Gas Gross Withdrawals from Oil Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 3,459 3,117 ...

  15. Missouri Natural Gas Gross Withdrawals from Oil Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    from Oil Wells (Million Cubic Feet) Missouri Natural Gas Gross Withdrawals from Oil Wells (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 ...

  16. Physics Nobel winner David Gross gives public lecture at Jefferson...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nobel winner David Gross gives public lecture at Jefferson Lab on June 12 (Monday) Physics ... "The Coming Revolutions in Fundamental Physics" beginning at 8 p.m. at Jefferson Lab on ...

  17. Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Federal Offshore--Gulf of Mexico Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov ...

  18. Montana Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Montana Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 4,561 3,826 4,106 ...

  19. Utah Natural Gas Gross Withdrawals from Gas Wells (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Utah Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 21,638 18,808 21,037 ...

  20. Oklahoma Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Oklahoma Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 7,051 6,368 ...

  1. Louisiana Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Louisiana Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 425,704 369,500 ...

  2. Florida Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Florida Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1996 - - - - - - - - - ...

  3. Ohio Natural Gas Gross Withdrawals from Shale Gas (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Ohio Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 1 1 1 1 1 1 1 1 1 1 ...

  4. Michigan Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Michigan Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 9,579 8,593 ...

  5. Montana Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Montana Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 1,239 1,119 1,239 ...

  6. Michigan Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Michigan Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 11,582 10,461 ...

  7. Virginia Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Virginia Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 1,622 1,465 ...

  8. Louisiana Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Louisiana Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 1,273 1,150 ...

  9. Oregon Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    from Gas Wells (Million Cubic Feet) Oregon Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 246 244 232 ...

  10. Colorado Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Colorado Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 11,749 10,612 ...

  11. Mississippi Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Mississippi Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 14,797 13,076 ...

  12. Wyoming Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Wyoming Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 58,111 51,244 ...

  13. Utah Natural Gas Gross Withdrawals from Shale Gas (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Utah Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 0 0 0 0 0 0 0 0 0 0 ...

  14. Wyoming Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Wyoming Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 331 299 331 320 ...

  15. Colorado Natural Gas Gross Withdrawals from Gas Wells (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Wells (Million Cubic Feet) Colorado Natural Gas Gross Withdrawals from Gas Wells (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 1991 15,390 18,697 ...

  16. Pennsylvania Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    from Shale Gas (Million Cubic Feet) Pennsylvania Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 0 0 0 0 ...

  17. Texas Natural Gas Gross Withdrawals from Shale Gas (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Shale Gas (Million Cubic Feet) Texas Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 107,415 97,020 ...

  18. Property:CoolingTowerWaterUseSummerGross | Open Energy Information

    Open Energy Info (EERE)

    Property Name CoolingTowerWaterUseSummerGross Property Type Number Description Cooling Tower Water use (summer average) (afday) Gross. Retrieved from "http:en.openei.orgw...

  19. Iowa: West Union Green Transformation Project | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    West Union Green Transformation Project Iowa: West Union Green Transformation Project ... These grant funds will be used to close up their buildings-making them more energy ...

  20. Union Temporal de Empresas UTE | Open Energy Information

    Open Energy Info (EERE)

    Temporal de Empresas UTE Jump to: navigation, search Name: Union Temporal de Empresas (UTE) Place: Spain Sector: Solar Product: Developer of PV solar parks. References: Union...

  1. Mutational, Structural, and Kinetic Evidence for a Dissociative Mechanism in the GDP-mannose Mannosyl Hydrolase Reaction

    SciTech Connect (OSTI)

    Xia,Z.; Azurmendi, H.; lairson, L.; Withers, S.; Gabelli, S.; Bianchet, M.; Amzel, L.; Mildvan, A.

    2005-01-01

    GDP-mannose hydrolase (GDPMH) catalyzes the hydrolysis of GDP-{alpha}-D-sugars by nucleophilic substitution with inversion at the anomeric C1 atom of the sugar, with general base catalysis by H124. Three lines of evidence indicate a mechanism with dissociative character. First, in the 1.3 Angstrom X-ray structure of the GDPMH-Mg{sup 2+}-GDP{center_dot}Tris{sup +} complex, the GDP leaving group interacts with five catalytic components: R37, Y103, R52, R65, and the essential Mg{sup 2+}. As determined by the effects of site-specific mutants on k{sub cat}, these components contribute factors of 24-, 100-, 309-, 24-, and {ge}10{sup 5}-fold, respectively, to catalysis. Both R37 and Y103 bind the {beta}-phosphate of GDP and are only 5.0 Angstroms apart. Accordingly, the R37Q/Y103F double mutant exhibits partially additive effects of the two single mutants on k{sub cat}, indicating cooperativity of R37 and Y103 in promoting catalysis, and antagonistic effects on K{sub m}. Second, the conserved residue, D22, is positioned to accept a hydrogen bond from the C2-OH group of the sugar undergoing substitution at C1, as was shown by modeling an {alpha}-D-mannosyl group into the sugar binding site. The D22A and D22N mutations decreased k{sub cat} by factors of 10{sup 2.1} and 10{sup 2.6}, respectively, for the hydrolysis of GDP-{alpha}-D-mannose, and showed smaller effects on K{sub m}, suggesting that the D22 anion stabilizes a cationic oxocarbenium transition state. Third, the fluorinated substrate, GDP-2F-{alpha}-D-mannose, for which a cationic oxocarbenium transition state would be destabilized by electron withdrawal, exhibited a 16-fold decrease in k{sub cat} and a smaller, 2.5-fold increase in K{sub m}. The D22A and D22N mutations further decreased the k{sub cat} with GDP-2F-{alpha}-D-mannose to values similar to those found with GDP-{alpha}-D-mannose, and decreased the K{sub m} of the fluorinated substrate. The choice of histidine as the general base over glutamate, the

  2. Deputy Secretary Roundtable Meeting with Unions | Department...

    Broader source: Energy.gov (indexed) [DOE]

    Meeting Agenda (39.25 KB) Meeting Summary (99.77 KB) Safety and Security Reform Plan (353.65 KB) Union Contact List (89.2 KB) More Documents & Publications Technical Standards ...

  3. AVIC Changli Union Energy | Open Energy Information

    Open Energy Info (EERE)

    Beijing Municipality, China Product: China-based JV to manufacture and sell zinc-air batteries. References: AVIC Changli Union Energy1 This article is a stub. You can help...

  4. Union, Ohio: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    is a stub. You can help OpenEI by expanding it. Union is a city in Miami County and Montgomery County, Ohio. It falls under Ohio's 8th congressional district and Ohio's 3rd...

  5. Union of Concerned Scientists | Open Energy Information

    Open Energy Info (EERE)

    http:www.ucsusa.org About "The Union of Concerned Scientists is the leading science-based nonprofit working for a healthy environment and a safer world. UCS combines...

  6. April 30, 2008; HSS/Union Working Group Meeting to address training - Union Participants

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    HSS/UNION MEETING: TRAINING HSS/Union Participants/Attendees HSS Participants: 1. Glenn Podonsky - Chief Health, Safety & Security Officer 2. Mari-Jo Campagnone - Senior Advisor to Chief HSS Officer 3. Bill Roege - Director, Corporate Safety Analysis 4. Pat Worthington - Director, Health and Safety 5. Bill McArthur - Director, Worker Safety & Health Policy 6. Tom Traceski - Director, Environmental Policy and Assistance 7. Jeff Harrell - Director, National Training Center [Call-in] UNION

  7. Helix Dipole Movement and Conformational Variability Contribute to Allosteric GDP Release in G[alpha] Subunits

    SciTech Connect (OSTI)

    Preininger, Anita M.; Funk, Michael A.; Oldham, William M.; Meier, Scott M.; Johnston, Christopher A.; Adhikary, Suraj; Kimple, Adam J.; Siderovski, David P.; Hamm, Heidi E.; Iverson, Tina M.

    2009-06-01

    Heterotrimeric G proteins (Galphabetagamma) transmit signals from activated G protein-coupled receptors (GPCRs) to downstream effectors through a guanine nucleotide signaling cycle. Numerous studies indicate that the carboxy-terminal alpha5 helix of Galpha subunits participates in Galpha-receptor binding, and previous EPR studies suggest this receptor-mediated interaction induces a rotation and translation of the alpha5 helix of the Galpha subunit [Oldham, W. M., et al. (2006) Nat. Struct. Mol. Biol. 13, 772-777]. On the basis of this result, an engineered disulfide bond was designed to constrain the alpha5 helix of Galpha(i1) into its EPR-measured receptor-associated conformation through the introduction of cysteines at position 56 in the alpha1 helix and position 333 in the alpha5 helix (I56C/Q333C Galpha(i1)). A functional mimetic of the EPR-measured alpha5 helix dipole movement upon receptor association was additionally created by introduction of a positive charge at the amino terminus of this helix, D328R Galpha(i1). Both proteins exhibit a dramatically elevated level of basal nucleotide exchange. The 2.9 A resolution crystal structure of I56C/Q333C Galpha(i1) in complex with GDP-AlF(4)(-) reveals the shift of the alpha5 helix toward the guanine nucleotide binding site that is anticipated by EPR measurements. The structure of the I56C/Q333C Galpha(i1) subunit further revealed altered positions for the switch regions and throughout the Galpha(i1) subunit, accompanied by significantly elevated crystallographic temperature factors. Combined with previous evidence in the literature, the structural analysis supports the critical role of electrostatics of the alpha5 helix dipole and overall conformational variability during nucleotide release.

  8. Gross alpha analytical modifications that improve wastewater treatment compliance

    SciTech Connect (OSTI)

    Tucker, B.J.; Arndt, S.

    2007-07-01

    This paper will propose an improvement to the gross alpha measurement that will provide more accurate gross alpha determinations and thus allow for more efficient and cost-effective treatment of site wastewaters. To evaluate the influence of salts that may be present in wastewater samples from a potentially broad range of environmental conditions, two types of efficiency curves were developed, each using a thorium-230 (Th-230) standard spike. Two different aqueous salt solutions were evaluated, one using sodium chloride, and one using salts from tap water drawn from the Bergen County, New Jersey Publicly Owned Treatment Works (POTW). For each curve, 13 to 17 solutions were prepared, each with the same concentration of Th-230 spike, but differing in the total amount of salt in the range of 0 to 100 mg. The attenuation coefficients were evaluated for the two salt types by plotting the natural log of the counted efficiencies vs. the weight of the sample's dried residue retained on the planchet. The results show that the range of the slopes for each of the attenuation curves varied by approximately a factor of 2.5. In order to better ensure the accuracy of results, and thus verify compliance with the gross alpha wastewater effluent criterion, projects depending on gross alpha measurements of environmental waters and wastewaters should employ gross alpha efficiency curves prepared with salts that mimic, as closely as possible, the salt content of the aqueous environmental matrix. (authors)

  9. Structure of the protein core of translation initiation factor 2 in apo, GTP-bound and GDP-bound forms

    SciTech Connect (OSTI)

    Simonetti, Angelita; Fabbretti, Attilio; Hazemann, Isabelle; Jenner, Lasse; Gualerzi, Claudio O.; Klaholz, Bruno P.

    2013-06-01

    The crystal structures of the eubacterial translation initiation factor 2 in apo form and with bound GDP and GTP reveal conformational changes upon nucleotide binding and hydrolysis, notably of the catalytically important histidine in the switch II region. Translation initiation factor 2 (IF2) is involved in the early steps of bacterial protein synthesis. It promotes the stabilization of the initiator tRNA on the 30S initiation complex (IC) and triggers GTP hydrolysis upon ribosomal subunit joining. While the structure of an archaeal homologue (a/eIF5B) is known, there are significant sequence and functional differences in eubacterial IF2, while the trimeric eukaryotic IF2 is completely unrelated. Here, the crystal structure of the apo IF2 protein core from Thermus thermophilus has been determined by MAD phasing and the structures of GTP and GDP complexes were also obtained. The IF2–GTP complex was trapped by soaking with GTP in the cryoprotectant. The structures revealed conformational changes of the protein upon nucleotide binding, in particular in the P-loop region, which extend to the functionally relevant switch II region. The latter carries a catalytically important and conserved histidine residue which is observed in different conformations in the GTP and GDP complexes. Overall, this work provides the first crystal structure of a eubacterial IF2 and suggests that activation of GTP hydrolysis may occur by a conformational repositioning of the histidine residue.

  10. Federal Offshore Louisiana Natural Gas Gross Withdrawals and...

    U.S. Energy Information Administration (EIA) Indexed Site

    Data Series Area 2009 2010 2011 2012 2013 2014 View History Gross Withdrawals NA NA NA 0 0 0 1977-2014 From Gas Wells NA NA NA 0 0 0 1977-2014 From Oil Wells NA NA NA 0 0 0 ...

  11. New Mexico Natural Gas Gross Withdrawals and Production

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals 1,341,475 1,287,682 1,276,296 1,247,394 1,265,579 1,289,908 1967-2015 From Gas Wells 616,134 556,024 653,057 588,127 535,181 1967-2014 From Oil Wells 238,580 ...

  12. Kansas Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 0 0 0 0 0 0 0 0 0 0 0 0 2008 0 0 0 0 0 0 0 0 0 0 0 0 ...

  13. Mississippi Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Mississippi Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 0 0 0 0 0 0 0 0 0 0 0 0 2008 0 0 0 0 0 0 0 0 ...

  14. Nebraska Natural Gas Gross Withdrawals from Shale Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Natural Gas Gross Withdrawals from Shale Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2007 0 0 0 0 0 0 0 0 0 0 0 0 2008 0 0 0 0 0 0 0 0 0 0 0 0 ...

  15. X-ray, NMR, and Mutational Studies of the Catalytic Cycle of the GDP-Mannose Mannosyl Hydrolase Reaction

    SciTech Connect (OSTI)

    Gabelli,S.; Azurmendi, H.; Bianchet, M.; Amzel, L.; Mildva, A.

    2006-01-01

    GDP-mannose hydrolase catalyzes the hydrolysis with inversion of GDP-{alpha}-D-hexose to GDP and {beta}-D-hexose by nucleophilic substitution by water at C1 of the sugar. Two new crystal structures (free enzyme and enzyme-substrate complex), NMR, and site-directed mutagenesis data, combined with the structure of the enzyme-product complex reported earlier, suggest a four-stage catalytic cycle. An important loop (L6, residues 119-125) contains a ligand to the essential Mg{sup 2+} (Gln-123), the catalytic base (His-124), and three anionic residues. This loop is not ordered in the X-ray structure of the free enzyme due to dynamic disorder, as indicated by the two-dimensional 1H-15N HMQC spectrum, which shows selective exchange broadening of the imidazole nitrogen resonances of His-124 (k{sub ex} = 6.6 x 10{sup 4} s{sup -1}). The structure of the enzyme-Mg{sup 2+}-GDP-mannose substrate complex of the less active Y103F mutant shows loop L6 in an open conformation, while the structure of the enzyme-Mg{sup 2+}-GDP product complex showed loop L6 in a closed, 'active' conformation. 1H-15N HMQC spectra show the imidazole N of His-124 to be unprotonated, appropriate for general base catalysis. Substituting Mg{sup 2+} with the more electrophilic metal ions Mn{sup 2+} or Co{sup 2+} decreases the pK{sub a} in the pH versus k{sub cat} rate profiles, showing that deprotonation of a metal-bound water is partially rate-limiting. The H124Q mutation, which decreases k{sub cat} 103.4-fold and largely abolishes its pH dependence, is rescued by the Y103F mutation, which increases k{sub cat} 23-fold and restores its pH dependence. The structural basis of the rescue is the fact that the Y103F mutation shifts the conformational equilibrium to the open form moving loop L6 out of the active site, thus permitting direct access of the specific base hydroxide from the solvent. In the proposed dissociative transition state, which occurs in the closed, active conformation of the enzyme, the

  16. Union Helps Produce Women Workers | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Union Helps Produce Women Workers Union Helps Produce Women Workers January 21, 2010 - 3:15pm Addthis Joshua DeLung At the Community Environmental Center in Long Island City, N.Y. ...

  17. Clay-Union Electric Coop | Open Energy Information

    Open Energy Info (EERE)

    Clay-Union Electric Coop Jump to: navigation, search Name: Clay-Union Electric Coop Place: South Dakota Phone Number: 605-624-2673 Website: www.clayunionelectric.coop Facebook:...

  18. EERE Success Story-Iowa: West Union Green Transformation Project...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    West Union Green Transformation Project EERE Success Story-Iowa: West Union Green ... These grant funds will be used to close up their buildings-making them more energy ...

  19. Property:CoolingTowerWaterUseAnnlAvgGross | Open Energy Information

    Open Energy Info (EERE)

    Property Name CoolingTowerWaterUseAnnlAvgGross Property Type Number Description Cooling Tower Water use (annual average) (afday) Gross. Retrieved from "http:en.openei.orgw...

  20. Property:CoolingTowerWaterUseWinterGross | Open Energy Information

    Open Energy Info (EERE)

    lingTowerWaterUseWinterGross Property Type Number Description Cooling Tower Water use (winter average) (afday) Gross. Retrieved from "http:en.openei.orgwindex.php?titleProper...

  1. U.S.-European Union Joint Statement | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    European Union Joint Statement U.S.-European Union Joint Statement U.S.-European Union Joint Statement prepared as part of the International Partnership for a Hydrogen Economy us_eu_hydrogen_summit_statement.pdf (61.18 KB) More Documents & Publications Microsoft Word - Document1 International Partnerships for the Hydrogen Economy Fact Sheet International Partnerships for the Hydrogen Economy Fact Sheet

  2. Louisiana Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals Total Offshore (Million Cubic Feet) Louisiana Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1970's 3,838,521 4,600,197 4,750,119 1980's 4,617,585 4,584,491 4,246,464 3,635,942 4,070,279 3,542,827 3,279,165 3,610,041 3,633,594 3,577,685 1990's 3,731,764 3,550,230 3,442,437 3,508,112 3,673,494 3,554,147 3,881,697 3,941,802 3,951,997 3,896,569 2000's 3,812,991 153,871 137,192 133,456

  3. Louisiana--onshore Natural Gas Gross Withdrawals (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Louisiana--onshore Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 1,535,033 1,538,511 1,552,603 1,608,633 1,469,698 1,357,155 1,386,478 1,434,389 2000's 1,342,963 1,370,802 1,245,270 1,244,672 1,248,050 1,202,328 1,280,758 1,309,960 1,301,523 1,482,252 2010's 2,148,447 2,969,297 2,882,193 2,289,193 1,925,968 - = No Data Reported; -- = Not Applicable; NA = Not Available;

  4. Alabama Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals Total Offshore (Million Cubic Feet) Alabama Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1980's 0 9 13 1990's 19,861 32,603 191,605 218,023 349,380 356,598 361,068 409,091 392,320 376,435 2000's 361,289 200,862 202,002 194,339 165,630 152,902 145,762 134,451 125,502 109,214 2010's 101,487 84,270 87,398 75,660 70,827 - = No Data Reported; -- = Not Applicable; NA = Not Available; W =

  5. Alabama--onshore Natural Gas Gross Withdrawals (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Alabama--onshore Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 222,009 228,298 229,483 223,527 221,233 220,674 212,470 207,863 2000's 200,255 191,119 184,500 176,571 173,106 164,304 160,381 155,167 152,051 146,751 2010's 139,215 134,305 128,312 120,666 110,226 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual

  6. Alaska Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals Total Offshore (Million Cubic Feet) Alaska Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1970's 72,813 71,946 1980's 63,355 71,477 66,852 68,776 68,315 62,454 63,007 69,656 101,440 122,595 1990's 144,064 171,665 216,377 233,198 224,301 113,552 126,051 123,854 133,111 125,841 2000's 263,958 262,937 293,580 322,010 334,125 380,568 354,816 374,204 388,188 357,490 2010's 370,148 364,702

  7. Alaska--onshore Natural Gas Gross Withdrawals (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Alaska--onshore Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 2,409,336 2,545,144 2,861,599 3,256,352 3,247,533 3,257,096 3,245,736 3,236,241 2000's 3,265,436 3,164,843 3,183,857 3,256,295 3,309,960 3,262,379 2,850,934 3,105,086 3,027,696 2,954,896 2010's 2,826,952 2,798,220 2,857,485 2,882,956 2,803,429 - = No Data Reported; -- = Not Applicable; NA = Not Available; W =

  8. Calif--onshore Natural Gas Gross Withdrawals (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Calif--onshore Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 386,382 346,733 334,987 322,544 326,919 317,137 315,701 347,667 2000's 334,983 336,629 322,138 303,480 287,205 291,271 301,921 286,584 281,088 258,983 2010's 273,136 237,388 214,509 219,386 218,512 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual

  9. California Natural Gas Gross Withdrawals Total Offshore (Million Cubic

    U.S. Energy Information Administration (EIA) Indexed Site

    Feet) Gross Withdrawals Total Offshore (Million Cubic Feet) California Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1970's 5,417 19,929 20,394 1980's 19,980 26,692 31,904 38,084 60,207 84,062 77,355 67,835 60,308 59,889 1990's 58,055 59,465 62,473 58,635 60,765 60,694 73,092 80,516 81,868 84,547 2000's 83,882 78,209 74,884 64,961 61,622 60,773 47,217 52,805 51,931 47,281 2010's 46,755 41,742

  10. Federal Offshore California Natural Gas Gross Withdrawals (Million Cubic

    U.S. Energy Information Administration (EIA) Indexed Site

    Feet) Gross Withdrawals (Million Cubic Feet) Federal Offshore California Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1970's 5,417 5,166 5,431 1980's 5,900 12,763 17,751 24,168 46,363 64,558 59,078 54,805 49,167 50,791 1990's 49,972 51,855 55,231 52,150 53,561 54,790 66,784 73,345 74,985 77,809 2000's 76,075 70,947 67,816 58,095 54,655 54,088 40,407 45,516 44,902 41,229 2010's 41,200 36,579 27,262 27,454

  11. Texas Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals Total Offshore (Million Cubic Feet) Texas Natural Gas Gross Withdrawals Total Offshore (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1970's 88,258 418,474 760,566 1980's 949,177 1,010,772 1,120,830 992,041 1,021,260 942,413 1,169,038 1,330,604 1,376,093 1,457,841 1990's 1,555,568 1,494,494 1,411,147 1,355,333 1,392,727 1,346,674 1,401,753 1,351,067 1,241,264 1,206,045 2000's 1,177,257 53,649 57,063 53,569 44,946 36,932 24,785

  12. Texas--onshore Natural Gas Gross Withdrawals (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    Gross Withdrawals (Million Cubic Feet) Texas--onshore Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1990's 5,296,865 5,461,594 5,518,978 5,525,982 5,626,448 5,665,074 5,738,595 5,526,033 2000's 5,681,726 5,698,798 5,603,941 5,737,755 5,688,972 5,969,905 6,301,649 6,931,629 7,753,869 7,615,836 2010's 7,565,123 7,910,898 8,127,004 8,285,436 8,652,111 - = No Data Reported; -- = Not Applicable; NA = Not Available; W =

  13. U.S.-European Union Joint Statement

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    JOINT STATEMENT BY PRESIDENT GEORGE W. BUSH, EUROPEAN COUNCIL PRESIDENT KONSTANDINOS SIMITIS, AND EUROPEAN COMMISSION PRESIDENT ROMANO PRODI ON THE HYDROGEN ECONOMY We affirm our commitment, on behalf of the United States and the European Union, to collaborate on accelerating the development of the hydrogen economy as part of our broadening cooperation on energy. We aim to enhance the security of energy supply, increase diversity of energy sources, and improve local and global environmental

  14. US - Former Soviet Union environmental management activities

    SciTech Connect (OSTI)

    1995-09-01

    The Office of Environmental Management (EM) has been delegated the responsibility for US DOE`s cleanup of nuclear weapons complex. The nature and the magnitude of the waste management and environmental remediation problem requires the identification of technologies and scientific expertise from domestic and foreign sources. This booklet makes comparisons and describes coordinated projects and workshops between the USA and the former Soviet Union.

  15. Conversion and enrichment in the Soviet Union

    SciTech Connect (OSTI)

    1991-04-01

    In the Soviet Union, just as in the West, the civilian nuclear industry emerged from research work undertaken for nuclear weapons development. At first, researchers tried various techniques for physical separation of uranium isotopes: electromagnetic and molecular-kinetic thermo-diffusion methods; gaseous diffusion; and centrifuge methods. All of those methods, which are based primarily on differences in the atomic mass of uranium isotopes, called for extensive research and the development of new, technically unprecedented equipment. Gradually gaseous diffusion and gas centrifuge technology became recognized as most feasible for industrial use, so research on other methods was terminated. Industrial-scale uranium enrichment in the Soviet Union began in 1949 using the gaseous diffusion method; by the early 1960s, centrifuge technology was in use on an industrial scale. All Soviet production of highly-enriched, weapons-grade uranium was halted in 1987. The Soviet Union now has four enrichment plants in operation (at classified locations), solely for civilian nuclear power needs. All four enrichment plants have centrifuge modules, and enrichment provided by gaseous diffusion accounts for less than 5% of their total output. Two of the four enrichment plants also incorporate facilities for conversion to uranium hexafluoride (UF{sub 6}).

  16. Physics Nobel winner David Gross gives public lecture at Jefferson Lab on

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    June 12 (Monday) | Jefferson Lab Nobel winner David Gross gives public lecture at Jefferson Lab on June 12 (Monday) Physics Nobel winner David Gross gives public lecture at Jefferson Lab on June 12 (Monday) June 6, 2006 David Gross David Gross, Nobel Prize recipient and lecturer David Gross, Nobel Prize recipient is scheduled to give a free, public lecture titled "The Coming Revolutions in Fundamental Physics" beginning at 8 p.m. at Jefferson Lab on (Monday) June 12. He is one of

  17. The Structure of the MUR1 GDP-mannose 4,67-deydratase from A. thaliana: Implications for Ligand Binding Specificity

    SciTech Connect (OSTI)

    Mulichak, A.M.; Bonin, C.P.; Reiter, W.-D.; Garavito, R.M.

    2010-03-08

    GDP-D-mannose 4,6-dehydratase catalyzes the first step in the de novo synthesis of GDP-L-fucose, the activated form of L-fucose, which is a component of glycoconjugates in plants known to be important to the development and strength of stem tissues. We have determined the three-dimensional structure of the MUR1 dehydratase isoform from Arabidopsis thaliana complexed with its NADPH cofactor as well as with the ligands GDP and GDP-D-rhamnose. MUR1 is a member of the nucleoside-diphosphosugar modifying subclass of the short-chain dehydrogenase/reductase enzyme family, having homologous structures and a conserved catalytic triad of Lys, Tyr, and Ser/Thr residues. MUR1 is the first member of this subfamily to be observed as a tetramer, the interface of which reveals a close and intimate overlap of neighboring NADP{sup +}-binding sites. The GDP moiety of the substrate also binds in an unusual syn conformation. The protein-ligand interactions around the hexose moiety of the substrate support the importance of the conserved triad residues and an additional Glu side chain serving as a general base for catalysis. Phe and Arg side chains close to the hexose ring may serve to confer substrate specificity at the O2 position. In the MUR1/GDP-D-rhamnose complex, a single unique monomer within the protein tetramer that has an unoccupied substrate site highlights the conformational changes that accompany substrate binding and may suggest the existence of negative cooperativity in MUR1 function.

  18. Gross Input to Atmospheric Crude Oil Distillation Units

    U.S. Energy Information Administration (EIA) Indexed Site

    Day) Process: Gross Input to Atmospheric Crude Oil Dist. Units Operable Capacity (Calendar Day) Operating Capacity Idle Operable Capacity Operable Utilization Rate Period: Monthly Annual Download Series History Download Series History Definitions, Sources & Notes Definitions, Sources & Notes Show Data By: Process Area Jan-16 Feb-16 Mar-16 Apr-16 May-16 Jun-16 View History U.S. 16,365 16,167 16,261 16,222 16,477 16,803 1985-2016 PADD 1 1,136 1,080 1,052 1,148 1,174 1,155 1985-2016 East

  19. California--State Offshore Natural Gas Gross Withdrawals (Million Cubic

    U.S. Energy Information Administration (EIA) Indexed Site

    Feet) Gross Withdrawals (Million Cubic Feet) California--State Offshore Natural Gas Gross Withdrawals (Million Cubic Feet) Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 1970's 14,763 14,963 1980's 14,080 13,929 14,153 13,916 13,844 19,504 18,277 13,030 11,141 9,098 1990's 8,083 7,610 7,242 6,484 7,204 5,904 6,309 7,171 6,883 6,738 2000's 7,808 7,262 7,068 6,866 6,966 6,685 6,809 7,289 7,029 6,052 2010's 5,554 5,163 5,051 5,470 5,961 - = No Data Reported; -- =

  20. Novel protein and Mg[superscript 2+] configurations in the Mg[superscript 2+] GDP Complex of the SRP GTPase ffh

    SciTech Connect (OSTI)

    Focia, Pamela J.; Alam, Hena; Lu, Thanh; Ramirez, Ursula D.; Freymann, Douglas M.

    2010-03-05

    Ffh is the signal sequence recognition and targeting subunit of the prokaryotic signal recognition particle (SRP). Previous structural studies of the NG GTPase domain of Ffh demonstrated magnesium-dependent and magnesium-independent binding conformations for GDP and GMPPNP that are believed to reflect novel mechanisms for exchange and activation in this member of the GTPase superfamily. The current study of the NG GTPase bound to Mg{sup 2+} GDP reveals two new binding conformations - in the first the magnesium interactions are similar to those seen previously, however, the protein undergoes a conformational change that brings a conserved aspartate into its second coordination sphere. In the second, the protein conformation is similar to that seen previously, but the magnesium coordination sphere is disrupted so that only five oxygen ligands are present. The loss of the coordinating water molecule, at the position that would be occupied by the oxygen of the {gamma}-phosphate of GTP, is consistent with that position being privileged for exchange during phosphate release. The available structures of the GDP-bound protein provide a series of structural snapshots that illuminate steps along the pathway of GDP release following GTP hydrolysis.

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