National Library of Energy BETA

Sample records for type incandescent fluorescent

  1. A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lights | Department of Energy A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent Lights A Bright Idea: New Efficiency Standards for Incandescent and Fluorescent Lights July 21, 2009 - 5:18pm Addthis John Lippert Pretty soon, lighting is going to get a lot more efficient. New standards for incandescent reflector bulbs, general purpose fluorescent bulbs, and regular incandescent bulbs are going into effect beginning in approximately three years. You may be curious about

  2. CALiPER Benchmark Report: Performance of Incandescent A Type and Decorative Lamps and LED Replacements

    SciTech Connect (OSTI)

    Lingard, R. D.; Myer, M. A.; Paget, M. L.

    2008-11-01

    This benchmark report addresses common omnidirectional incandescent lamps - A-type and small decorative, candelabra-type lamps - and their commercially available light-emitting diode (LED) replacements.

  3. Incandescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lighting » Incandescent Lighting Incandescent Lighting Incandescent lighting is the most common, and least energy efficient, type of lighting used in homes. | Photo courtesy of ©iStockphoto/TokenPhoto. Incandescent lighting is the most common, and least energy efficient, type of lighting used in homes. | Photo courtesy of ©iStockphoto/TokenPhoto. Incandescent lamps are often considered the least energy efficient type of electric lighting commonly found in residential buildings. Although

  4. ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen"

    U.S. Energy Information Administration (EIA) Indexed Site

    B39. Lighting Equipment, Floorspace, 1999" ,"Total Floorspace (million square feet)" ,"All Buildings","All Lit Buildings","Lighting Equipment (more than one may apply)" ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen" "All Buildings ................",67338,64321,38156,60344,20666,19223,17926 "Building Floorspace" "(Square

  5. ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen"

    U.S. Energy Information Administration (EIA) Indexed Site

    8. Lighting Equipment, Number of Buildings, 1999" ,"Number of Buildings (thousand)" ,"All Buildings","All Lit Buildings","Lighting Equipment (more than one may apply)" ,,,"Incandescent","Standard Fluorescent","Compact Fluorescent","High-Intensity Discharge","Halogen" "All Buildings ................",4657,4172,2193,3778,607,430,572 "Building Floorspace" "(Square Feet)"

  6. 2014-04-11 Issuance: Energy Conservation Standards for General Service Fluorescent Lamps and Incandescent Reflector Lamps; Notice of Proposed Rulemaking

    Broader source: Energy.gov [DOE]

    This document is a pre-publication Federal Register notice of proposed rulemaking regarding energy conservation standards for general service fluorescent lamps and incandescent reflectors lamps, as issued by the Assistant Secretary for Energy Efficiency and Renewable Energy on April 11, 2014.

  7. Incandescent Lighting | Department of Energy

    Office of Environmental Management (EM)

    courtesy of iStockphotoTokenPhoto. Incandescent lamps are often considered the least energy efficient type of electric lighting commonly found in residential buildings....

  8. Types of Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    selection. Types of lighting include: Fluorescent Incandescent Outdoor solar Light-emitting diode (LED) Also learn how energy-efficient lightbulbs compare to traditional...

  9. Photonically Engineered Incandescent Emitter

    DOE Patents [OSTI]

    Gee, James M.; Lin, Shawn-Yu; Fleming, James G.; Moreno, James B.

    2005-03-22

    A photonically engineered incandescence is disclosed. The emitter materials and photonic crystal structure can be chosen to modify or suppress thermal radiation above a cutoff wavelength, causing the emitter to selectively emit in the visible and near-infrared portions of the spectrum. An efficient incandescent lamp is enabled thereby. A method for fabricating a three-dimensional photonic crystal of a structural material, suitable for the incandescent emitter, is also disclosed.

  10. Photonically engineered incandescent emitter

    DOE Patents [OSTI]

    Gee, James M.; Lin, Shawn-Yu; Fleming, James G.; Moreno, James B.

    2003-08-26

    A photonically engineered incandescence is disclosed. The emitter materials and photonic crystal structure can be chosen to modify or suppress thermal radiation above a cutoff wavelength, causing the emitter to selectively emit in the visible and near-infrared portions of the spectrum. An efficient incandescent lamp is enabled thereby. A method for fabricating a three-dimensional photonic crystal of a structural material, suitable for the incandescent emitter, is also disclosed.

  11. High efficiency incandescent lighting

    DOE Patents [OSTI]

    Bermel, Peter; Ilic, Ognjen; Chan, Walker R.; Musabeyoglu, Ahmet; Cukierman, Aviv Ruben; Harradon, Michael Robert; Celanovic, Ivan; Soljacic, Marin

    2014-09-02

    Incandescent lighting structure. The structure includes a thermal emitter that can, but does not have to, include a first photonic crystal on its surface to tailor thermal emission coupled to, in a high-view-factor geometry, a second photonic filter selected to reflect infrared radiation back to the emitter while passing visible light. This structure is highly efficient as compared to standard incandescent light bulbs.

  12. Fluorescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Electricity & Fuel » Lighting » Fluorescent Lighting Fluorescent Lighting Fluorescent Lighting Fluorescent lamps use 25%-35% of the energy used by incandescent products to provide a similar amount of light. They also last about 10 times longer (7,000-24,000 hours). The two general types of fluorescent lamps are: Compact fluorescent lamps (CFLs) -- commonly found with integral ballasts and screw bases, these are popular lamps often used in household fixtures Fluorescent tube and circline

  13. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    induced incandescence (LII) - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced

  14. Incandescent Lighting Basics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Incandescent Lighting Basics Incandescent Lighting Basics August 16, 2013 - 10:00am Addthis Incandescent lamps consist of a wire filament inside a glass bulb that is usually filled with inert gas, and they produce light when an electric current heats the filament to a high temperature. Incandescent lamps have a low efficacy (10-17 lumens per watt) compared with other lighting options-because most of the energy released is in the form of heat rather than light-and a short average operating life

  15. Energy-Saving Incandescents | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    lightbulbs - high-resolution EPS (2.15 MB) More Documents & Publications Energy-Saving Incandescents CFL Lightbulbs CFL Lightbulbs

  16. How Energy-Efficient Light Bulbs Compare with Traditional Incandescent...

    Office of Environmental Management (EM)

    Energy-Efficient Light Bulbs Compare with Traditional Incandescents How Energy-Efficient Light Bulbs Compare with Traditional Incandescents November 5, 2014 - 11:39pm Addthis By...

  17. Fluorescent Lighting | Department of Energy

    Office of Environmental Management (EM)

    - by far the most common form of fluorescent lighting but rarely found in residential buildings -- are much more energy efficient than incandescent lamps and are ideally suited...

  18. Dual LED/incandescent security fixture

    DOE Patents [OSTI]

    Gauna, Kevin Wayne

    2005-06-21

    A dual LED and incandescent security lighting system uses a hybrid approach to LED illumination. It combines an ambient LED illuminator with a standard incandescent lamp on a motion control sensor. The LED illuminator will activate with the onset of darkness (daylight control) and typically remain on during the course of the night ("always on"). The LED illumination, typically amber, is sufficient to provide low to moderate level lighting coverage to the wall and ground area adjacent to and under the fixture. The incandescent lamp is integrated with a motion control circuit and sensor. When movement in the field of view is detected (after darkness), the incandescent lamp is switched on, providing an increased level of illumination to the area. Instead of an "always on" LED illuminator, the LEDs may also be switched off when the incandescent lamp is switched on.

  19. Energy-Saving Incandescents | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    lighbulbs - high-resolution JPG (3.85 MB) More Documents & Publications Energy-Saving Incandescents CFL Lightbulbs Which Bulb Is Right for You? (High-Resolution JPG Billboard)

  20. Energy Cost Calculator for Compact Fluorescent Lamps | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Compact Fluorescent Lamps Energy Cost Calculator for Compact Fluorescent Lamps This tool calculates the payback period for your calc retrofit project. Modify the default values to suit your project requirements. Existing incandescent lamp wattage Watts Incandescent lamp cost dollars Incandescent lamp life 1000 hours calc wattage Watts calc cost dollars calc life (6000 hours for moderate use, 10000 hours for high use) 8000 hours Number of lamps in retrofit project Hours operating per week hours

  1. Operating temperatures for a convectively cooled recessed incandescent light fixture

    SciTech Connect (OSTI)

    Yarbrough, D.W.; Toor, I.

    1980-12-01

    Test results are given for the operation of a recessed incandescent light fixture intended for residential use. The fixture is labeled for use in direct contact with attic thermal insulation. Temperature control of the powered fixture is provided by convective heat transfer from the ceiling side of the fixture. The fixture was operated at power levels up to two times the rated power of 75 watts and under thermal insulations up to R-40. In all operating configurations tested the fixture surface in contact with attic insulation was found to be less than 175/sup 0/C. The observed surface temperatures are judged to be safe for operation in contact with loose-fill or batt-type insulations. It was observed that the power leads inside one fixture configuration are exposed to temperatures as high as 168/sup 0/C. The electrical insulation could, therefore, have a limited life. The properties of the internal fixture wiring were not, however, studied in detail.

  2. How Energy-Efficient Light Bulbs Compare with Traditional Incandescents |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Electricity & Fuel » Lighting » How Energy-Efficient Light Bulbs Compare with Traditional Incandescents How Energy-Efficient Light Bulbs Compare with Traditional Incandescents By replacing your home's five most frequently used light fixtures or bulbs with models that have earned the ENERGY STAR, you can save $75 each year. By replacing your home's five most frequently used light fixtures or bulbs with models that have earned the ENERGY STAR, you can save $75 each

  3. Fluorescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    They also last about 10 times longer (7,000-24,000 hours). The two general types of ... Although CFLs cost a bit more than comparable incandescent bulbs, they last 6-15 times as ...

  4. Convection venting lensed reflector-type compact fluorescent lamp system

    DOE Patents [OSTI]

    Pelton, Bruce A.; Siminovitch, Michael

    1997-01-01

    Disclosed herein is a fluorescent lamp housing assembly capable of providing convection cooling to the lamp and the ballast. The lens of the present invention includes two distinct portions, a central portion and an apertured portion. The housing assembly further includes apertures so that air mass is able to freely move up through the assembly and out ventilation apertures.

  5. Convection venting lensed reflector-type compact fluorescent lamp system

    DOE Patents [OSTI]

    Pelton, B.A.; Siminovitch, M.

    1997-07-29

    Disclosed herein is a fluorescent lamp housing assembly capable of providing convection cooling to the lamp and the ballast. The lens of the present invention includes two distinct portions, a central portion and an apertured portion. The housing assembly further includes apertures so that air mass is able to freely move up through the assembly and out ventilation apertures. 12 figs.

  6. EA-1911: Energy Conservation Standards for Certain Reflector, Elliptical Reflector, and Bulged Reflector Incandescent Lamps

    Broader source: Energy.gov [DOE]

    This EA will evaluate the environmental impacts of a proposal to amend energy conservation standards for Certain Reflector, Elliptical Reflector, and Bulged Reflector Incandescent Lamps.

  7. DOE Publishes Final Rule for the Request for Exclusion of 100 Watt R20 Short Incandescent Reflector Lamps from Energy Conservation Standards

    Broader source: Energy.gov [DOE]

    The Department of Energy has published a final rule regarding the request for exclusion of 100 Watt R20 short incandescent reflector lamps from energy conservation standards.

  8. Effects of aggregate morphology and size on laser-induced incandescence and scattering from black carbon (mature soot)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bambha, Ray P.; Michelsen, Hope A.

    2015-07-03

    We have used a Single-Particle Soot Photometer (SP2) to measure time-resolved laser-induced incandescence (LII) and laser scatter from combustion-generated mature soot with a fractal dimension of 1.88 extracted from a burner. We have also made measurements on restructured mature-soot particles with a fractal dimension of 2.3–2.4. We reproduced the LII and laser-scatter temporal profiles with an energy- and mass-balance model, which accounted for heating of particles passed through a CW-laser beam over laser–particle interaction times of ~10 μs. Furthermore, the results demonstrate a strong influence of aggregate size and morphology on LII and scattering signals. Conductive cooling competes with absorptivemore » heating on these time scales; the effects are reduced with increasing aggregate size and fractal dimension. These effects can lead to a significant delay in the onset of the LII signal and may explain an apparent low bias in the SP2 measurements for small particle sizes, particularly for fresh, mature soot. The results also reveal significant perturbations to the measured scattering signal from LII interference and suggest rapid expansion of the aggregates during sublimation.« less

  9. Effects of repetitive pulsing on multi-kHz planar laser-induced incandescence imaging in laminar and turbulent flames

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Michael, James B.; Venkateswaran, Prabhakar; Shaddix, Christopher R.; Meyer, Terrence R.

    2015-04-08

    Planar laser-induced incandescence (LII) imaging is reported at repetition rates up to 100 kHz using a burst-mode laser system to enable studies of soot formation dynamics in highly turbulent flames. Furthermore, to quantify the accuracy and uncertainty of relative soot volume fraction measurements, the temporal evolution of the LII field in laminar and turbulent flames is examined at various laser operating conditions. Under high-speed repetitive probing, it is found that LII signals are sensitive to changes in soot physical characteristics when operating at high laser fluences within the soot vaporization regime. For these laser conditions, strong planar LII signals aremore » observed at measurement rates up to 100 kHz but are primarily useful for qualitative tracking of soot structure dynamics. However, LII signals collected at lower fluences allow sequential planar measurements of the relative soot volume fraction with a sufficient signal-to-noise ratio at repetition rates of 10–50 kHz. Finally, guidelines for identifying and avoiding the onset of repetitive probe effects in the LII signals are discussed, along with other potential sources of measurement error and uncertainty.« less

  10. Quantification of online removal of refractory black carbon using laser-induced incandescence in the single particle soot photometer

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Aiken, Allison C.; McMeeking, Gavin R.; Levin, Ezra J. T.; Dubey, Manvendra K.; DeMott, Paul J.; Kreidenweis, Sonia M.

    2016-04-05

    Refractory black carbon (rBC) is an aerosol that has important impacts on climate and human health. rBC is often mixed with other species, making it difficult to isolate and quantify its important effects on physical and optical properties of ambient aerosol. To solve this measurement challenge, a new method to remove rBC was developed using laser-induced incandescence (LII) by Levin et al. in 2014. Application of the method with the Single Particle Soot Photometer (SP2) is used to determine the effects of rBC on ice nucleating particles (INP). Here, we quantify the efficacy of the method in the laboratory usingmore » the rBC surrogate Aquadag. Polydisperse and mobility-selected samples (100–500 nm diameter, 0.44–36.05 fg), are quantified by a second SP2. Removal rates are reported by mass and number. For the mobility-selected samples, the average percentages removed by mass and number of the original size are 88.9 ± 18.6% and 87.3 ± 21.9%, respectively. Removal of Aquadag is efficient for particles >100 nm mass-equivalent diameter (dme), enabling application for microphysical studies. However, the removal of particles ≤100 nm dme is less efficient. Absorption and scattering measurements are reported to assess its use to isolate brown carbon (BrC) absorption. Scattering removal rates for the mobility-selected samples are >90% on average, yet absorption rates are 53% on average across all wavelengths. Therefore, application to isolate effects of microphysical properties determined by larger sizes is promising, but will be challenging for optical properties. Lastly, the results reported also have implications for other instruments employing internal LII, e.g., the Soot Particle Aerosol Mass Spectrometer (SP-AMS).« less

  11. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, Richard I.; Edwards, Bradley C.; Buchwald, Melvin I.; Gosnell, Timothy R.

    1995-01-01

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement.

  12. Fluorescent refrigeration

    DOE Patents [OSTI]

    Epstein, R.I.; Edwards, B.C.; Buchwald, M.I.; Gosnell, T.R.

    1995-09-05

    Fluorescent refrigeration is based on selective radiative pumping, using substantially monochromatic radiation, of quantum excitations which are then endothermically redistributed to higher energies. Ultimately, the populated energy levels radiatively deexcite emitting, on the average, more radiant energy than was initially absorbed. The material utilized to accomplish the cooling must have dimensions such that the exciting radiation is strongly absorbed, but the fluorescence may exit the material through a significantly smaller optical pathlength. Optical fibers and mirrored glasses and crystals provide this requirement. 6 figs.

  13. Untitled

    Gasoline and Diesel Fuel Update (EIA)

    0. Number of Lights by Bulb Type by Room, 1993 Bulb Type Incandescent Fluorescent Other Room Total Low Medium High Unknown Short Long Compact Halogen Other unknown Total 4,196 431...

  14. Fluorescent Nanoparticles for Radiation DetectionFluorescent...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Find More Like This Return to Search Fluorescent Nanoparticles for Radiation DetectionFluorescent Nanoparticles for Radiation Detection Oak Ridge National Laboratory Contact ORNL ...

  15. Compact Fluorescent Plug-In Ballast-in-a-Socket

    SciTech Connect (OSTI)

    Rebecca Voelker

    2001-12-21

    The primary goal of this program was to develop a ballast system for plug-in CFLs (compact fluorescent lamps) that will directly replace standard metal shell, medium base incandescent lampholders (such as Levition No. 6098) for use with portable lamp fixtures, such as floor, table and desk lamps. A secondary goal was to identify a plug-in CFL that is optimized for use with this ballast. This Plug-in CFL Ballastin-a-Socket system will allow fixture manufacturers to easily manufacture CFL-based high-efficacy portable fixtures that provide residential and commercial consumers with attractive, cost-effective, and energy-efficient fixtures for use wherever portable incandescent fixtures are used today. The advantages of this proposed system over existing CFL solutions are that the fixtures can only be used with high-efficacy CFLs, and they will be more attractive and will have lower life-cycle costs than screw-in or adapter-based CFL retrofit solutions. These features should greatly increase the penetration of CFL's into the North American market. Our work has shown that using integrated circuits it is quite feasible to produce a lamp-fixture ballast of a size comparable to the current Edison-screw 3-way incandescent fixtures. As for price points for BIAS-based fixtures, end-users polled by the Lighting Research Institute at RPI indicated that they would pay as much as an additional $10 for a lamp containing such a ballast. The ballast has been optimized to run with a 26 W amalgam triple biax lamp in the base-down position, yet can accept non-amalgam versions of the lamp. With a few part alterations, the ballast can be produced to support 32 W lamps as well. The ballast uses GE's existing L-Comp[1] power topology in the circuit so that the integrated circuit design would be a design that could possibly be used by other CFL and EFL products with minor modifications. This gives added value by reducing cost and size of not only the BIAS, but also possibly other integral

  16. Adoption of Light-Emitting Diodes in Common Lighting Applications

    SciTech Connect (OSTI)

    Yamada, Mary; Chwastyk, Dan

    2013-05-01

    Report estimating LED energy savings in nine applications where LEDs compete with traditional lighting sources such as incandescent, halogen, high-pressure sodium, and certain types of fluorescent. The analysis includes indoor lamp, indoor luminaire, and outdoor luminaire applications.

  17. PowerPoint Presentation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    41 51 61 INCANDESCENT HALOGEN CFL LED 0 500 1,000 1,500 2,000 2,500 COUNCIL BASELINE MARKET AVERAGE TYPE: CURRENT PRACTICE BASELINE EFFICACY Linear Fluorescent Lamps, 4ft...

  18. Fluorescence detection: SPIE volume 743

    SciTech Connect (OSTI)

    Menzel, E.R.

    1987-01-01

    This book contains proceedings arranged into four sessions. They are: Fluorescence spectroscopic techniques; Fluorescence in analysis and materials characterization; Fluorescence in medicine and biochemistry; and Fluorescence in criminalistics.

  19. EA-1664: Final Environmental Assessment

    Office of Energy Efficiency and Renewable Energy (EERE)

    10 CFR Part 430 Energy Conservation Standards: Energy Conservation Standards for Fluorescent and Incandescent Lamps

  20. Energy 101: Lighting Choices | Department of Energy

    Energy Savers [EERE]

    but with higher efficiencies-so they save you money. Upgrading 15 of the inefficient incandescent light bulbs in your home to energy-saving incandescent, compact fluorescent ...

  1. Sustainable LED Fluorescent Light Replacement Technology

    SciTech Connect (OSTI)

    2011-06-30

    Ilumisys and the National Center for Manufacturing Sciences (NCMS) partnered on a three-year project awarded by the United States (U.S.) Department of Energy (DOE), to quantify the impacts of LED lamps, incandescent lamps and fluorescent benchmark lamps over a product lifecycle – i.e. to develop a sustainable design and manufacturing strategy that addresses product manufacturing, use, recycling and disposal scenarios for LED-based lighting. Based on the knowledge gained from extensive product tear-down studies of fluorescent and screw-in lighting products, lifecycle assessment tools, and accelerated lifecycle testing protocols, an interactive Sustainable LED Design Guide has been developed to aid architectural and lighting designers and engineers in making design decisions that consider three important environmental impacts (greenhouse gas emissions, energy use and mercury emission) across all phases of the life of an LED lighting product. Critical information developed for the lifecycle analysis and product feature comparisons is the useful life of the lighting product as well as its performance. The Design Guide is available at www.ncms.org, and was developed based on operational and durability testing of a variety of lighting products including power consumption, light output, and useful life of a lamp in order to allow a more realistic comparison of lamp designs. This report describes the main project tasks, results and innovative features of the lifecycle assessment (LCA)-based design tools, and the key considerations driving the sustainable design of LED lighting systems. The Design Guide incorporates the following three novel features for efficiently evaluating LED lighting features in value-chains: • Bill-of-Materials (BOM) Builder – Designers may import process data for each component and supply functional data for the product, including power, consumption, lumen output and expected useful life. • Environmental Impact Review – Designs are

  2. Fluorescent optical position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  3. Safe biodegradable fluorescent particles

    DOE Patents [OSTI]

    Martin, Sue I.; Fergenson, David P.; Srivastava, Abneesh; Bogan, Michael J.; Riot, Vincent J.; Frank, Matthias

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  4. Types of Lighting in Commercial Buildings - Lighting Types

    U.S. Energy Information Administration (EIA) Indexed Site

    is termed fluorescence). A ballast is required to regulate and control the current and voltage. Two types of ballasts are used, magnetic and electronic. Electronic ballasts have...

  5. Laser-induced incandescence (LII)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Mountain Gondola to see the amazing views of the mountain and the lake at the top. ... folklore, statistics, landmarks, beaches, movies, and secret sports of Lake Tahoe. ...

  6. Incandescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    These lamps produce visible light by heating a tiny coil or filament of tungsten wire that glows when it is heated by an electrical current. "Long-life" lamps are an example of ...

  7. Fluorescent fiber diagnostics

    DOE Patents [OSTI]

    Toeppen, John S.

    1994-10-04

    A fluorescent fiber (13) having a doped core (16) is pumped (11) by light (18) of a relatively short wavelength to produce fluorescence at a longer wavelength that is detected by detector (24). The level of fluorescence is monitored (26) and evaluated to provide information as to the excitation of the fiber (13) or the environment thereof. In particular, the level of intensity of the detected fluorescence may be used to measure the intensity of a light beam (18) passing axially through an optical fiber system (12) (FIG. 1 ), or the intensity of a light beam (46) passing radially through a fluorescent fiber (13) (FIG. 2 ), or the level of a fluid (32) in a tank (31) (FIG. 3 ), or a scintillation event (37) in a fluorescent fiber (13) pumped to produce amplification of the scintillation event (FIG. 4 ).

  8. Fluorescent fiber diagnostics

    DOE Patents [OSTI]

    Toeppen, John S.

    1994-01-01

    A fluorescent fiber (13) having a doped core (16) is pumped (11) by light (18) of a relatively short wavelength to produce fluorescence at a longer wavelength that is detected by detector (24). The level of fluorescence is monitored (26) and evaluated to provide information as to the excitation of the fiber (13) or the environment thereof. In particular, the level of intensity of the detected fluorescence may be used to measure the intensity of a light beam (18) passing axially through an optical fiber system (12) (FIG. 1 ), or the intensity of a light beam (46) passing radially through a fluorescent fiber (13) (FIG. 2 ), or the level of a fluid (32) in a tank (31) (FIG. 3 ), or a scintillation event (37) in a fluorescent fiber (13) pumped to produce amplification of the scintillation event (FIG. 4 ).

  9. Organimetallic Fluorescent Complex Polymers For Light Emitting Applications

    DOE Patents [OSTI]

    Shi, Song Q.; So, Franky

    1997-10-28

    A fluorescent complex polymer with fluorescent organometallic complexes connected by organic chain spacers is utilized in the fabrication of light emitting devices on a substantially transparent planar substrate by depositing a first conductive layer having p-type conductivity on the planar surface of the substrate, depositing a layer of a hole transporting and electron blocking material on the first conductive layer, depositing a layer of the fluorescent complex polymer on the layer of hole transporting and electron blocking material as an electron transporting emissive layer and depositing a second conductive layer having n-type conductivity on the layer of fluorescent complex polymer.

  10. Fluorescent Tube Lamps

    Broader source: Energy.gov [DOE]

    FEMP temporarily suspended its energy efficiency requirements for fluorescent tube lamps as it evaluates the market impact of the pending 2012 minimum efficiency standards for fluorescent lamps. The program will issue updated energy efficiency requirements when the market distribution of this product category stabilizes and when doing so has the potential to result in significant Federal energy savings.

  11. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  12. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-11-29

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  13. Highly thermostable fluorescent proteins

    DOE Patents [OSTI]

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2012-05-01

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  14. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen; Sun, Yiru; Giebink, Noel; Thompson, Mark E.

    2010-08-03

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  15. Fluorescent filtered electrophosphorescence

    DOE Patents [OSTI]

    Forrest, Stephen R.; Sun, Yiru; Giebink, Noel; Thompson, Mark E.

    2009-01-06

    The present invention relates to organic light emitting devices (OLEDs), and more specifically to OLEDS that emit light using a combination of fluorescent emitters and phosphorescent emitters for the efficient utilization of all of the electrically generated excitons.

  16. Recombinant fluorescent protein microsphere calibration standard

    DOE Patents [OSTI]

    Nolan, John P.; Nolan, Rhiannon L.; Ruscetti, Teresa; Lehnert, Bruce E.

    2001-01-01

    A method for making recombinant fluorescent protein standard particles for calibration of fluorescence instruments.

  17. Fluorescent image tracking velocimeter

    DOE Patents [OSTI]

    Shaffer, Franklin D.

    1994-01-01

    A multiple-exposure fluorescent image tracking velocimeter (FITV) detects and measures the motion (trajectory, direction and velocity) of small particles close to light scattering surfaces. The small particles may follow the motion of a carrier medium such as a liquid, gas or multi-phase mixture, allowing the motion of the carrier medium to be observed, measured and recorded. The main components of the FITV include: (1) fluorescent particles; (2) a pulsed fluorescent excitation laser source; (3) an imaging camera; and (4) an image analyzer. FITV uses fluorescing particles excited by visible laser light to enhance particle image detectability near light scattering surfaces. The excitation laser light is filtered out before reaching the imaging camera allowing the fluoresced wavelengths emitted by the particles to be detected and recorded by the camera. FITV employs multiple exposures of a single camera image by pulsing the excitation laser light for producing a series of images of each particle along its trajectory. The time-lapsed image may be used to determine trajectory and velocity and the exposures may be coded to derive directional information.

  18. Electric Power | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    From incandescent bulbs to fluorescents to LEDs, learn more about the long history of the light bulb. From incandescent bulbs to fluorescents to LEDs,...

  19. Simultaneous neuron- and astrocyte-specific fluorescent marking

    SciTech Connect (OSTI)

    Schulze, Wiebke; Hayata-Takano, Atsuko; Kamo, Toshihiko; Nakazawa, Takanobu; Nagayasu, Kazuki; Kasai, Atsushi; Seiriki, Kaoru; Shintani, Norihito; Ago, Yukio; Farfan, Camille; and others

    2015-03-27

    Systematic and simultaneous analysis of multiple cell types in the brain is becoming important, but such tools have not yet been adequately developed. Here, we aimed to generate a method for the specific fluorescent labeling of neurons and astrocytes, two major cell types in the brain, and we have developed lentiviral vectors to express the red fluorescent protein tdTomato in neurons and the enhanced green fluorescent protein (EGFP) in astrocytes. Importantly, both fluorescent proteins are fused to histone 2B protein (H2B) to confer nuclear localization to distinguish between single cells. We also constructed several expression constructs, including a tandem alignment of the neuron- and astrocyte-expression cassettes for simultaneous labeling. Introducing these vectors and constructs in vitro and in vivo resulted in cell type-specific and nuclear-localized fluorescence signals enabling easy detection and distinguishability of neurons and astrocytes. This tool is expected to be utilized for the simultaneous analysis of changes in neurons and astrocytes in healthy and diseased brains. - Highlights: • We develop a method for the specific fluorescent labeling of neurons and astrocytes. • Neuron-specific labeling is achieved using Scg10 and synapsin promoters. • Astrocyte-specific labeling is generated using the minimal GFAP promoter. • Nuclear localization of fluorescent proteins is achieved with histone 2B protein.

  20. Integrated fluorescence analysis system

    DOE Patents [OSTI]

    Buican, Tudor N.; Yoshida, Thomas M.

    1992-01-01

    An integrated fluorescence analysis system enables a component part of a sample to be virtually sorted within a sample volume after a spectrum of the component part has been identified from a fluorescence spectrum of the entire sample in a flow cytometer. Birefringent optics enables the entire spectrum to be resolved into a set of numbers representing the intensity of spectral components of the spectrum. One or more spectral components are selected to program a scanning laser microscope, preferably a confocal microscope, whereby the spectrum from individual pixels or voxels in the sample can be compared. Individual pixels or voxels containing the selected spectral components are identified and an image may be formed to show the morphology of the sample with respect to only those components having the selected spectral components. There is no need for any physical sorting of the sample components to obtain the morphological information.

  1. Magnetic fluorescent lamp

    DOE Patents [OSTI]

    Berman, S.M.; Richardson R.W.

    1983-12-29

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  2. Laser-Induced Fluorescence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Induced Fluorescence - Sandia Energy Energy Search Icon Sandia Home Locations Contact Us Employee Locator Energy & Climate Secure & Sustainable Energy Future Stationary Power Energy Conversion Efficiency Solar Energy Wind Energy Water Power Supercritical CO2 Geothermal Natural Gas Safety, Security & Resilience of the Energy Infrastructure Energy Storage Nuclear Power & Engineering Grid Modernization Battery Testing Nuclear Energy Defense Waste Management Programs Advanced Nuclear

  3. Types of Lighting in Commercial Buildings - Changes

    U.S. Energy Information Administration (EIA) Indexed Site

    of lit buildings to more than 20 percent (Figure 17 and Table 5). The use of halogen lamps nearly doubled, from 7 percent to 13 percent of lit buildings. Use of incandescent...

  4. Fluorescent microthermographic imaging

    SciTech Connect (OSTI)

    Barton, D.L.

    1993-09-01

    In the early days of microelectronics, design rules and feature sizes were large enough that sub-micron spatial resolution was not needed. Infrared or IR thermal techniques were available that calculated the object`s temperature from infrared emission. There is a fundamental spatial resolution limitation dependent on the wavelengths of light being used in the image formation process. As the integrated circuit feature sizes began to shrink toward the one micron level, the limitations imposed on IR thermal systems became more pronounced. Something else was needed to overcome this limitation. Liquid crystals have been used with great success, but they lack the temperature measurement capabilities of other techniques. The fluorescent microthermographic imaging technique (FMI) was developed to meet this need. This technique offers better than 0.01{degrees}C temperature resolution and is diffraction limited to 0.3 {mu}m spatial resolution. While the temperature resolution is comparable to that available on IR systems, the spatial resolution is much better. The FMI technique provides better spatial resolution by using a temperature dependent fluorescent film that emits light at 612 nm instead of the 1.5 {mu}m to 12 {mu}m range used by IR techniques. This tutorial starts with a review of blackbody radiation physics, the process by which all heated objects emit radiation to their surroundings, in order to understand the sources of information that are available to characterize an object`s surface temperature. The processes used in infrared thermal imaging are then detailed to point out the limitations of the technique but also to contrast it with the FMI process. The FMI technique is then described in detail, starting with the fluorescent film physics and ending with a series of examples of past applications of FMI.

  5. Fluorescent temperature sensor

    DOE Patents [OSTI]

    Baker, Gary A [Los Alamos, NM; Baker, Sheila N [Los Alamos, NM; McCleskey, T Mark [Los Alamos, NM

    2009-03-03

    The present invention is a fluorescent temperature sensor or optical thermometer. The sensor includes a solution of 1,3-bis(1-pyrenyl)propane within a 1-butyl-1-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ionic liquid solvent. The 1,3-bis(1-pyrenyl)propane remains unassociated when in the ground state while in solution. When subjected to UV light, an excited state is produced that exists in equilibrium with an excimer. The position of the equilibrium between the two excited states is temperature dependent.

  6. Fluorescence analyzer for lignin

    DOE Patents [OSTI]

    Berthold, John W.; Malito, Michael L.; Jeffers, Larry

    1993-01-01

    A method and apparatus for measuring lignin concentration in a sample of wood pulp or black liquor comprises a light emitting arrangement for emitting an excitation light through optical fiber bundles into a probe which has an undiluted sensing end facing the sample. The excitation light causes the lignin concentration to produce fluorescent emission light which is then conveyed through the probe to analyzing equipment which measures the intensity of the emission light. Measures a This invention was made with Government support under Contract Number DOE: DE-FC05-90CE40905 awarded by the Department of Energy (DOE). The Government has certain rights in this invention.

  7. X-ray fluorescence mapping

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    X-Ray Microscopy and Imaging: X-ray Fluorescence Mapping Of increasing scientific interest is the detection, quantification and mapping of elemental content of samples, often down...

  8. Fluorescent fluid interface position sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D.

    2004-02-17

    A new fluid interface position sensor has been developed, which is capable of optically determining the location of an interface between an upper fluid and a lower fluid, the upper fluid having a larger refractive index than a lower fluid. The sensor functions by measurement, of fluorescence excited by an optical pump beam which is confined within a fluorescent waveguide where that waveguide is in optical contact with the lower fluid, but escapes from the fluorescent waveguide where that waveguide is in optical contact with the upper fluid.

  9. Untitled

    U.S. Energy Information Administration (EIA) Indexed Site

    position of the fixture affect the actual output of compact fluorescent bulbs, and the quality of the light may differ between compact fluorescents and incandescents....

  10. Types of Lighting in Commercial Buildings - Table L3

    U.S. Energy Information Administration (EIA) Indexed Site

    Any Lighting Lighted Area Only Area Lit by Each Type of Light Incan- descent Standard Fluor-escent Compact Fluor- escent High Intensity Discharge Halogen All...

  11. Types of Lighting in Commercial Buildings - Table L1

    U.S. Energy Information Administration (EIA) Indexed Site

    Any Lighting Lighted Area Only Area Lit by Each Type of Light Incan- descent Standard Fluor-escent Compact Fluor- escent High Intensity Discharge Halogen All...

  12. Types of Lighting in Commercial Buildings - Table L2

    U.S. Energy Information Administration (EIA) Indexed Site

    Any Lighting Lighted Area Only Area Lit by Each Type of Light Incan- descent Standard Fluor-escent Compact Fluor- escent High Intensity Discharge Halogen All Buildings*...

  13. Fluorescent Lighting Basics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lighting Basics Fluorescent Lighting Basics October 17, 2013 - 5:39pm Addthis Light from a fluorescent lamp is first created by an electric current conducted through an inert gas producing ultraviolet light that is invisible to the human eye. The ultraviolet light in turn interacts with special blends of phosphors coating the interior surface of the fluorescent lamp tube that efficiently converts the invisible light into useful white light. Fluorescent lamps require a special power

  14. Fluorescent Lighting Basics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lighting Basics Fluorescent Lighting Basics October 17, 2013 - 5:39pm Addthis Light from a fluorescent lamp is first created by an electric current conducted through an inert gas producing ultraviolet light that is invisible to the human eye. The ultraviolet light in turn interacts with special blends of phosphors coating the interior surface of the fluorescent lamp tube that efficiently converts the invisible light into useful white light. Fluorescent lamps require a special power

  15. Covered Product Category: Fluorescent Luminaires

    Broader source: Energy.gov [DOE]

    FEMP provides acquisition guidance and Federal efficiency requirements across a variety of product categories, including fluorescent luminaires. Federal laws and requirements mandate that agencies meet these efficiency requirements in all procurement and acquisition actions that are not specifically exempted by law.

  16. 2014-12-30 Issuance: Energy Conservation Standard for General Service

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Lamps and Incandescent Reflector Lamps; Final Rule | Department of Energy 30 Issuance: Energy Conservation Standard for General Service Fluorescent Lamps and Incandescent Reflector Lamps; Final Rule 2014-12-30 Issuance: Energy Conservation Standard for General Service Fluorescent Lamps and Incandescent Reflector Lamps; Final Rule This document is a pre-publication Federal Register final rule regarding energy conservation standards for general service fluorescent lamps and

  17. Covered Product Category: Fluorescent Luminaires | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Luminaires," using IESNA LM-41, ANSI C82.2-2002 for fluorescent ballasts, and ANSI C78.81-2005 for fluorescent lamps. Finding the Luminaire Efficacy Rating LERs should be...

  18. Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers

    DOE Patents [OSTI]

    Walsh, Joseph C.; Heiss, Aaron M.; Noronha, Glenn; Vachon, David J.; Lane, Stephen M.; Satcher, Jr., Joe H.; Peyser, Thomas A.; Van Antwerp, William Peter; Mastrototaro, John Joseph

    2004-07-20

    Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

  19. Fluorescent optical liquid level sensor

    DOE Patents [OSTI]

    Weiss, Jonathan D. (Albuquerque, NM)

    2001-01-01

    A liquid level sensor comprising a transparent waveguide containing fluorescent material that is excited by light of a first wavelength and emits at a second, longer wavelength. The upper end of the waveguide is connected to a light source at the first wavelength through a beveled portion of the waveguide such that the input light is totally internally reflected within the waveguide above an air/liquid interface in a tank but is transmitted into the liquid below this interface. Light is emitted from the fluorescent material only in those portions of the waveguide that are above the air/liquid interface, to be collected at the upper end of the waveguide by a detector that is sensitive only to the second wavelength. As the interface moves down in the tank, the signal strength from the detector will increase.

  20. Fluorescence based chemical sensors for corrosion detection

    SciTech Connect (OSTI)

    Johnson, R.E.; Agarwala, V.S.

    1997-12-01

    Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal surfaces for the detection of corrosion. Oxine reacts with aluminum oxide on corroded aluminum to give a fluorescence that can be photographed in UV light. Several other materials were found to have good fluorescence but cannot be reversibly oxidized or reduced at the present time. More work will be done with these compounds as well as with Schiff bases to develop new fluorescent chemical sensing materials for smart coating on alloy surfaces.

  1. Compact fast analyzer of rotary cuvette type

    DOE Patents [OSTI]

    Thacker, Louis H.

    1976-01-01

    A compact fast analyzer of the rotary cuvette type is provided for simultaneously determining concentrations in a multiplicity of discrete samples using either absorbance or fluorescence measurement techniques. A rigid, generally rectangular frame defines optical passageways for the absorbance and fluorescence measurement systems. The frame also serves as a mounting structure for various optical components as well as for the cuvette rotor mount and drive system. A single light source and photodetector are used in making both absorbance and fluorescence measurements. Rotor removal and insertion are facilitated by a swing-out drive motor and rotor mount. BACKGROUND OF THE INVENTION The invention relates generally to concentration measuring instruments and more specifically to a compact fast analyzer of the rotary cuvette type which is suitable for making either absorbance or fluorescence measurements. It was made in the course of, or under, a contract with the U.S. Atomic Energy Commission.

  2. Chromosome characterization using single fluorescent dye

    DOE Patents [OSTI]

    Crissman, Harry A.; Hirons, Gregory T.

    1995-01-01

    Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.

  3. Saccharide sensing molecules having enhanced fluorescent properties

    DOE Patents [OSTI]

    Satcher Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-06

    The present invention provides formulae for fluorescent compounds that have a number of properties which make them uniquely suited for use in sensors of analytes such as saccharides. The advantageous fluorescent properties include favorable excitation wavelengths, emission wavelengths, fluorescence lifetimes, and photostability. Additional advantageous properties include enhanced aqueous solubility, as well as temperature and pH sensitivity. The compound comprises an aryl or a substituted phenyl botonic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  4. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, Daniel L.; Tangyunyong, Paiboon

    1998-01-01

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC.

  5. Scanning fluorescent microthermal imaging apparatus and method

    DOE Patents [OSTI]

    Barton, D.L.; Tangyunyong, P.

    1998-01-06

    A scanning fluorescent microthermal imaging (FMI) apparatus and method is disclosed, useful for integrated circuit (IC) failure analysis, that uses a scanned and focused beam from a laser to excite a thin fluorescent film disposed over the surface of the IC. By collecting fluorescent radiation from the film, and performing point-by-point data collection with a single-point photodetector, a thermal map of the IC is formed to measure any localized heating associated with defects in the IC. 1 fig.

  6. Optical gating of perylene bisimide fluorescence usingdithienylcyclop...

    Office of Scientific and Technical Information (OSTI)

    The emission of millions of fluorescence photons from a chromophore is controlled by the absorption of a few tens of photons in a photochromic molecule. The parameters that ...

  7. Portable spotter for fluorescent contaminants on surfaces

    DOE Patents [OSTI]

    Schuresko, Daniel D.

    1980-01-01

    A portable fluorescence-based spotter for polynuclear aromatic hydrocarbon contamination on personnel and work area surfaces under ambient lighting conditions is provided. This instrument employs beam modulation and phase sensitive detection for discriminating between fluorescence from organic materials from reflected background light and inorganic fluorescent material. The device uses excitation and emission filters to provide differentiation between classes of aromatic organic compounds. Certain inorganic fluorescent materials, including heavy metal compounds, may also be distinguished from the organic compounds, despite both having similar optical properties.

  8. Fluorescent Optical Position Sensor - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technologies Find More Like This Return to Search Fluorescent Optical Position Sensor Sandia National Laboratories Contact SNL About This Technology Publications: PDF...

  9. Fermentative Method for Making Nonoxide Fluorescent Nanoparticles...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fermentative Method for Making Nonoxide Fluorescent Nanoparticles (Quantum Dots) Oak Ridge National Laboratory Contact ORNL About This Technology Incubation of quantum dots...

  10. LED Replacements for Linear Fluorescent Lamps Webcast

    Broader source: Energy.gov [DOE]

    In this June 20, 2011 webcast on LED products marketed as replacements for linear fluorescent lamps, Jason Tuenge of the Pacific Northwest National Laboratory (PNNL) discussed current Lighting...

  11. 2014-04-11 Issuance: Energy Conservation Standards for General...

    Energy Savers [EERE]

    Energy Conservation Standards for General Service Fluorescent Lamps and Incandescent Reflector Lamps; Notice of Proposed Rulemaking 2014-04-11 Issuance: Energy Conservation ...

  12. Buying an Appliance this Holiday Season? ENERGY STAR Products...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Refrigerators Freezers Room air conditioners Televisions Clothes washers Dishwashers Battery chargers Water heaters Fluorescent lamp ballasts Incandescent reflector lamps If your ...

  13. Save Energy With A Picnic! | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    the physical effort it takes to power incandescent, compact fluorescent and LED light bulbs. Students from Churchill Road Elementary School in Virginia recently pedaled for...

  14. APPLIANCE STANDARDS

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Equipment General Service Incandescent Lamps General Service Fluorescent Lamps Small Electric Motors Electric Motors Water Heaters Furnace Fans Distribution Transformers Water...

  15. Peering into a Quantum Well | U.S. DOE Office of Science (SC...

    Office of Science (SC) Website

    to this potentially energy-saving alternative to conventional incandescent and fluorescent lighting. At issue, in particular, are the blue (and green) nitride-based ...

  16. National Lighting Energy Consumption

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lighting Energy National Lighting Energy Consumption Consumption 390 Billion kWh used for lighting in all 390 Billion kWh used for lighting in all commercial buildings in commercial buildings in 2001 2001 LED (<.1% ) Incandescent 40% HID 22% Fluorescent 38% Lighting Energy Consumption by Lighting Energy Consumption by Breakdown of Lighting Energy Breakdown of Lighting Energy Major Sector and Light Source Type Major Sector and Light Source Type Source: Navigant Consulting, Inc., U.S. Lighting

  17. Lighting In the Library: A Student Energy Audit

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Homes & Buildings » Lighting & Daylighting » Lighting Basics Lighting Basics August 15, 2013 - 5:12pm Addthis Text Version There are many different types of artificial lights (formally called "lamps" in the lighting industry,) which have different applications and uses. Types of lighting include: Fluorescent Lighting High-intensity Discharge Lighting Incandescent Lighting LED Lighting. New lamp designs that use energy-efficient technology are now readily available in the

  18. Lighting Basics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Homes & Buildings » Lighting & Daylighting » Lighting Basics Lighting Basics August 15, 2013 - 5:12pm Addthis Text Version There are many different types of artificial lights (formally called "lamps" in the lighting industry,) which have different applications and uses. Types of lighting include: Fluorescent Lighting High-intensity Discharge Lighting Incandescent Lighting LED Lighting. New lamp designs that use energy-efficient technology are now readily available in the

  19. Applications of Nuclear Resonance Fluorescence

    SciTech Connect (OSTI)

    Warren, Glen A.; Detwiler, Rebecca S.; Peplowski, Patrick N.

    2010-11-11

    Nuclear Resonance Fluorescence (NRF) has the potential of addressing a wide variety of applications, which require isotopic and/or elemental information about a sample. We have investigated a variety of non-proliferation applications that may be addressed by NRF. From these applications, we have selected two, measuring uranium enrichment in UF6 cylinders and material verification in dismantlement, to investigate in more detail. Analytical models have been developed to evaluate these applications, and test measurements have been conducted to validate those models. We found that it is unlikely with current technology to address the requirements for UF6 cylinder enrichment measurements. In contrast, NRF is a very promising approach for material verification for dismantlement.

  20. Fiber optical assembly for fluorescence spectrometry

    DOE Patents [OSTI]

    Carpenter, II, Robert W.; Rubenstein, Richard; Piltch, Martin; Gray, Perry

    2010-12-07

    A system for analyzing a sample for the presence of an analyte in a sample. The system includes a sample holder for containing the sample; an excitation source, such as a laser, and at least one linear array radially disposed about the sample holder. Radiation from the excitation source is directed to the sample, and the radiation induces fluorescent light in the sample. Each linear array includes a plurality of fused silica optical fibers that receive the fluorescent light and transmits a fluorescent light signal from the first end to an optical end port of the linear array. An end port assembly having a photo-detector is optically coupled to the optical end port. The photo-detector detects the fluorescent light signal and converts the fluorescent light signal into an electrical signal.

  1. Glucose sensing molecules having selected fluorescent properties

    DOE Patents [OSTI]

    Satcher, Jr., Joe H.; Lane, Stephen M.; Darrow, Christopher B.; Cary, Douglas R.; Tran, Joe Anh

    2004-01-27

    An analyte sensing fluorescent molecule that employs intramolecular electron transfer is designed to exhibit selected fluorescent properties in the presence of analytes such as saccharides. The selected fluorescent properties include excitation wavelength, emission wavelength, fluorescence lifetime, quantum yield, photostability, solubility, and temperature or pH sensitivity. The compound comprises an aryl or a substituted phenyl boronic acid that acts as a substrate recognition component, a fluorescence switch component, and a fluorophore. The fluorophore and switch component are selected such that the value of the free energy for electron transfer is less than about 3.0 kcal mol.sup.-1. Fluorescent compounds are described that are excited at wavelengths greater than 400 nm and emit at wavelengths greater than 450 nm, which is advantageous for optical transmission through skin. The fluorophore is typically selected from transition metal-ligand complexes and thiazine, oxazine, oxazone, or oxazine-one as well as anthracene compounds. The fluorescent compound can be immobilized in a glucose permeable biocompatible polymer matrix that is implantable below the skin.

  2. Simultaneous cryo X-ray ptychographic and fluorescence microscopy...

    Office of Scientific and Technical Information (OSTI)

    Accepted Manuscript: Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae Prev Next Title: Simultaneous cryo X-ray ptychographic and fluorescence ...

  3. Fluorescent single walled nanotube/silica composite materials...

    Office of Scientific and Technical Information (OSTI)

    Title: Fluorescent single walled nanotubesilica composite materials Fluorescent composites of surfactant-wrapped single-walled carbon nanotubes (SWNTs) were prepared by exposing ...

  4. Fluorescent Pigments for High-Performance Cool Roofing and Facades...

    Energy Savers [EERE]

    After boiling and then combustion, a fluorescent red pigment is formed. Image: University ... After boiling and then combustion, a fluorescent red pigment is formed. Image: University ...

  5. Rigidifying Fluorescent Linkers by Metal-Organic Framework Formation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Rigidifying Fluorescent Linkers by Metal-Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement Previous Next List Zhangwen Wei, Zhi-Yuan Gu, Ravi K. ...

  6. Product Standards for Fluorescent Lighting (Japan) | Open Energy...

    Open Energy Info (EERE)

    Fluorescent Lighting (Japan) Jump to: navigation, search Tool Summary LAUNCH TOOL Name: Product Standards for Fluorescent Lighting (Japan) Focus Area: Appliances & Equipment...

  7. 2014-10-14 Issuance: Test Procedures Correction for Fluorescent...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Issuance: Test Procedures Correction for Fluorescent Lamp Ballasts; Notice of Proposed Rulemaking 2014-10-14 Issuance: Test Procedures Correction for Fluorescent Lamp Ballasts; ...

  8. " A Heterodyne Laser-induced Fluorescence Technique to Determine...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Heterodyne Laser-induced Fluorescence Technique to Determine Simultaneously the Bulk ... molecule velocity distribution using a heterodyne laser induced fluorescence technique. ...

  9. Development of fluorescence lifetime diagnostic. Project accomplishments

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    summary (Attachment 1), Revision 1 (Technical Report) | SciTech Connect Technical Report: Development of fluorescence lifetime diagnostic. Project accomplishments summary (Attachment 1), Revision 1 Citation Details In-Document Search Title: Development of fluorescence lifetime diagnostic. Project accomplishments summary (Attachment 1), Revision 1 × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and

  10. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, Edward S.; Taylor, John A.

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  11. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, Edward S.; Taylor, John A.

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis.

  12. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1996-03-12

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.

  13. Multiplexed fluorescence detector system for capillary electrophoresis

    DOE Patents [OSTI]

    Yeung, E.S.; Taylor, J.A.

    1994-06-28

    A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figures.

  14. Laser excited confocal microscope fluorescence scanner and method

    DOE Patents [OSTI]

    Mathies, Richard A.; Peck, Konan

    1992-01-01

    A fluorescent scanner for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier including a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from said volume to provide a display of the separated sample.

  15. Laser excited confocal microscope fluorescence scanner and method

    DOE Patents [OSTI]

    Mathies, R.A.; Peck, K.

    1992-02-25

    A fluorescent scanner is designed for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier. The scanner includes a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from the volume to provide a display of the separated sample. 8 figs.

  16. Ultratrace analysis of transuranic actinides by laser-induced fluorescence

    DOE Patents [OSTI]

    Miller, S.M.

    1983-10-31

    Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

  17. Ultratrace analysis of transuranic actinides by laser-induced fluorescence

    DOE Patents [OSTI]

    Miller, Steven M.

    1988-01-01

    Ultratrace quantities of transuranic actinides are detected indirectly by their effect on the fluorescent emissions of a preselected fluorescent species. Transuranic actinides in a sample are coprecipitated with a host lattice material containing at least one preselected fluorescent species. The actinide either quenches or enhances the laser-induced fluorescence of the preselected fluorescent species. The degree of enhancement or quenching is quantitatively related to the concentration of actinide in the sample.

  18. Award Types

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Awards Team (505) 667-7824 Email Types of Awards The Awards Office, sponsored by the Technology Transfer Division and the Science and Technology Base Program Office, coordinates...

  19. Ultrabright fluorescent OLEDS using triplet sinks

    DOE Patents [OSTI]

    Zhang, Yifan; Forrest, Stephen R; Thompson, Mark

    2013-06-04

    A first device is provided. The first device further comprises an organic light emitting device. The organic light emitting device further comprises an anode, a cathode, and an emissive layer disposed between the anode and the cathode. The emissive layer further comprises an organic host compound, an organic emitting compound capable of fluorescent emission at room temperature, and an organic dopant compound. The triplet energy of the dopant compound is lower than the triplet energy of the host compound. The dopant compound does not strongly absorb the fluorescent emission of the emitting compound.

  20. Buildings Energy Data Book: 5.6 Lighting

    Buildings Energy Data Book [EERE]

    7 2003 Lighted Floorspace for the Stock of Commercial Buildings, by Type of Lamp (1) Type of Lamp (Billion SF) (2) Standard Fluorescent 59.7 96% Incandescent 38.5 62% Compact Fluorescent 27.6 44% High-Intensity Discharge 20.6 33% Halogen 17.7 29% Note(s): Source(s): EIA, 2003 Commercial Buildings Energy Consumption Survey: Building Characteristics Tables, June 2006, Table B44, p. 220. Lighted Floorspace Percent of Total Lighted Floorspace: 62.06 Billion SF Lighted Floorspace 1) Mall buildings

  1. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, K.D.; Chu, T.J.; Pitt, W.G.

    1992-05-12

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through amino groups contained on the surface. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to the target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membranes may be reprobed numerous times. No Drawings

  2. DNA sequencing using fluorescence background electroblotting membrane

    DOE Patents [OSTI]

    Caldwell, Karin D.; Chu, Tun-Jen; Pitt, William G.

    1992-01-01

    A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

  3. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are aminomore » acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.« less

  4. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria

    SciTech Connect (OSTI)

    Hill, Steven C.; Williamson, Chatt C.; Doughty, David C.; Pan, Yong-Le; Santarpia, Joshua L.; Hill, Hanna H.

    2015-02-02

    This paper uses a mathematical model of fluorescent biological particles composed of bacteria and/or proteins (mostly as in Hill et al., 2013 [23]) to investigate the size-dependence of the total fluorescence emitted in all directions. The model applies to particles which have negligible reabsorption of fluorescence within the particle. The specific particles modeled here are composed of ovalbumin and of a generic Bacillus. The particles need not be spherical, and in some cases need not be homogeneous. However, the results calculated in this paper are for spherical homogeneous particles. Light absorbing and fluorescing molecules included in the model are amino acids, nucleic acids, and several coenzymes. Here the excitation wavelength is 266 nm. The emission range, 300 to 370 nm, encompasses the fluorescence of tryptophan. The fluorescence cross section (CF) is calculated and compared with one set of published measured values. We investigate power law (Ady) approximations to CF, where d is diameter, and A and y are parameters adjusted to fit the data, and examine how y varies with d and composition, including the fraction as water. The particle's fluorescence efficiency (QF=CF/geometric-cross-section) can be written for homogeneous particles as QabsRF, where Qabs is the absorption efficiency, and RF, the fraction of the absorbed light emitted as fluorescence, is independent of size and shape. When QF is plotted vs. mid or mi(mr-1)d, where m=mr+imi is the complex refractive index, the plots for different fractions of water in the particle tend to overlap.

  5. Apparatus for eliminating background interference in fluorescence measurements

    DOE Patents [OSTI]

    Martin, J.C.; Jett, J.H.

    1986-03-04

    The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle. 8 figs.

  6. Apparatus for eliminating background interference in fluorescence measurements

    DOE Patents [OSTI]

    Martin, John C.; Jett, James H.

    1986-01-01

    The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser, which excites a different stained component of the same biological particle.

  7. Apparatus for eliminating background interference in fluorescence measurements

    DOE Patents [OSTI]

    Martin, J.C.; Jett, J.H.

    1984-01-06

    The disclosure is directed to an apparatus for eliminating background interference during fluorescence measurements in a multiple laser flow cytometer. A biological particle stained with fluorescent dyes is excited by a laser. A fluorescence detector detects the fluorescence. The particle scatters light and a gate signal is generated and delayed until the biological particle reaches the next laser. The delayed signal turns on this next laser which excites a different stained component of the same biological particle.

  8. A Bright Idea: New Efficiency Standards for Incandescent and...

    Office of Environmental Management (EM)

    July 21, 2009 - 5:18pm Addthis John Lippert Pretty soon, lighting is going to get a lot ... trying to adopt more energy-efficient lighting; it has also spurred media articles ...

  9. How Energy-Efficient Light Bulbs Compare with Traditional Incandescent...

    Energy Savers [EERE]

    Light Bulbs Compare with Traditional ... The table below compares a 60 watt (W) traditional ... *Based on 2 hrsday of usage, an electricity rate of 11 ...

  10. DOE Requires Manufacturer and Labeler to Cease Sale of Incandescent...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Non-Compliance Determination to Westinghouse Lighting Corporation and Fuzhou Sunlight Lighting Electrical Appliance Company requiring that they halt the sale of 8 basic...

  11. Type: Renewal

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 INCITE Awards Type: Renewal Title: -Ab Initio Dynamical Simulations for the Prediction of Bulk Properties‖ Principal Investigator: Theresa Windus, Iowa State University Co-Investigators: Brett Bode, Iowa State University Graham Fletcher, Argonne National Laboratory Mark Gordon, Iowa State University Monica Lamm, Iowa State University Michael Schmidt, Iowa State University Scientific Discipline: Chemistry: Physical INCITE Allocation: 10,000,000 processor hours Site: Argonne National

  12. Facility Type!

    Office of Legacy Management (LM)

    ITY: --&L~ ----------- srct-r~ -----------~------~------- if yee, date contacted ------------- cl Facility Type! i I 0 Theoretical Studies Cl Sample 84 Analysis ] Production 1 Diepasal/Storage 'YPE OF CONTRACT .--------------- 1 Prime J Subcontract&- 1 Purchase Order rl i '1 ! Other information (i.e., ---------~---~--~-------- :ontrait/Pirchaee Order # , I C -qXlJ- --~-------~~-------~~~~~~ I I ~~~---~~~~~~~T~~~ FONTRACTING PERIODi IWNERSHIP: ,I 1 AECIMED AECMED GOVT GOUT &NTtiAC+OR

  13. Invisible-fluorescent identification tags for materials

    SciTech Connect (OSTI)

    Lewis, Linda A.; Allgood, Glenn O.; Smithwick, III, Robert W.

    2013-03-26

    A taggant composition including a taggant material that is invisible in light of the visible spectrum and fluoresces under a non-visible excitation energy, a binder, and a solvent in which the taggant material and the binder are dissolved. The taggant composition can be printed or otherwise applied to a material such as fabric to provide a detectable and identifiable indicium. A method and apparatus for detecting and decoding the taggant indicium are also provided.

  14. Fluorescent lighting with aluminum nitride phosphors

    DOE Patents [OSTI]

    Cherepy, Nerine J.; Payne, Stephen A.; Seeley, Zachary M.; Srivastava, Alok M.

    2016-05-10

    A fluorescent lamp includes a glass envelope; at least two electrodes connected to the glass envelope; mercury vapor and an inert gas within the glass envelope; and a phosphor within the glass envelope, wherein the phosphor blend includes aluminum nitride. The phosphor may be a wurtzite (hexagonal) crystalline structure Al.sub.(1-x)M.sub.xN phosphor, where M may be drawn from beryllium, magnesium, calcium, strontium, barium, zinc, scandium, yttrium, lanthanum, cerium, praseodymium, europium, gadolinium, terbium, ytterbium, bismuth, manganese, silicon, germanium, tin, boron, or gallium is synthesized to include dopants to control its luminescence under ultraviolet excitation. The disclosed Al.sub.(1-x)M.sub.xN:Mn phosphor provides bright orange-red emission, comparable in efficiency and spectrum to that of the standard orange-red phosphor used in fluorescent lighting, Y.sub.2O.sub.3:Eu. Furthermore, it offers excellent lumen maintenance in a fluorescent lamp, and does not utilize "critical rare earths," minimizing sensitivity to fluctuating market prices for the rare earth elements.

  15. Type here

    Office of Environmental Management (EM)

    Injury at the Savannah River National Laboratory | Department of Energy January 10, 2006, Flash Fire and Injury at the Savannah River National Laboratory Type B Accident Investigation of the January 10, 2006, Flash Fire and Injury at the Savannah River National Laboratory February 1, 2006 On January 10, 2006, at approximately 7:47 a.m., a first-line manager (FLM) at the Savannah River National Laboratory (SRNL) received first- and second-degree burns to his head, face, neck, and left hand

  16. Preliminary evaluation of a fluorescence and radioisotope nuclear smuggling deterrence tag - final report (IL500E)

    SciTech Connect (OSTI)

    Hartenstein, S.D.; Aryaeinejad, R.; Delmastro, J.R.

    1997-04-01

    This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials, (2) inventory control of long-term stored items containing special nuclear materials, and (3) tracking materials transferred between facilities. The system uses three types of materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. This report also summarizes the efforts completed in identifying hardware that will be used for the tagging system. This hardware includes the devices for applying the tagging materials, the commercially available fluorescence detection systems, and gamma ray detection systems assembled from existing, commercially available technologies.

  17. Superior optical nonlinearity of an exceptional fluorescent stilbene dye

    SciTech Connect (OSTI)

    He, Tingchao; Sreejith, Sivaramapanicker; Zhao, Yanli; Gao, Yang; Grimsdale, Andrew C.; Lin, Xiaodong E-mail: hdsun@ntu.edu.sg; Sun, Handong E-mail: hdsun@ntu.edu.sg

    2015-03-16

    Strong multiphoton absorption and harmonic generation in organic fluorescent chromophores are, respectively, significant in many fields of research. However, most of fluorescent chromophores fall short of the full potential due to the absence of the combination of such different nonlinear upconversion behaviors. Here, we demonstrate that an exceptional fluorescent stilbene dye could exhibit efficient two- and three-photon absorption under the excitation of femtosecond pulses in solution phase. Benefiting from its biocompatibility and strong excited state absorption behavior, in vitro two-photon bioimaging and superior optical limiting have been exploited, respectively. Simultaneously, the chromophore could generate efficient three-photon excited fluorescence and third-harmonic generation (THG) when dispersed into PMMA film, circumventing the limitations of classical fluorescent chromophores. Such chromophore may find application in the production of coherent light sources of higher photon energy. Moreover, the combination of three-photon excited fluorescence and THG can be used in tandem to provide complementary information in biomedical studies.

  18. Solid state laser media driven by remote nuclear powered fluorescence

    DOE Patents [OSTI]

    Prelas, Mark A.

    1992-01-01

    An apparatus is provided for driving a solid state laser by a nuclear powered fluorescence source which is located remote from the fluorescence source. A nuclear reaction produced in a reaction chamber generates fluorescence or photons. The photons are collected from the chamber into a waveguide, such as a fiber optic waveguide. The waveguide transports the photons to the remote laser for exciting the laser.

  19. Lighting the Way with Compact Fluorescent Lighting | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lighting the Way with Compact Fluorescent Lighting Lighting the Way with Compact Fluorescent Lighting April 28, 2009 - 5:00am Addthis John Lippert There is a major push today to get homeowners to adopt compact fluorescent lamp (CFL) light bulbs. They have been on the market for nearly three decades, and many homeowners still do not use them widely. But the tide is definitely turning. Their availability and the percentage of homeowners familiar with the technology and purchasing them for their

  20. Purchasing Energy-Efficient Ceiling-Mounted Fluorescent Luminaires |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Ceiling-Mounted Fluorescent Luminaires Purchasing Energy-Efficient Ceiling-Mounted Fluorescent Luminaires The Federal Energy Management Program (FEMP) provides acquisition guidance for ceiling-mounted fluorescent luminaires, a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR-qualified or FEMP-designated products in all product categories covered by these programs and in any acquisition

  1. Purchasing Energy-Efficient Fluorescent Ballasts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fluorescent Ballasts Purchasing Energy-Efficient Fluorescent Ballasts The Federal Energy Management Program (FEMP) provides acquisition guidance for fluorescent ballasts, a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR-qualified or FEMP-designated products in all product categories covered by these programs and in any acquisition actions that are not specifically exempted by law. FEMP's acquisition guidance and

  2. Purchasing Energy-Efficient General Service Fluorescent Lamps | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Purchasing Energy-Efficient General Service Fluorescent Lamps Purchasing Energy-Efficient General Service Fluorescent Lamps The Federal Energy Management Program (FEMP) provides acquisition guidance for general service fluorescent lamps (GSFLs), a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR qualified or FEMP designated products in all product categories covered by these programs and in any

  3. Purchasing Energy-Efficient Suspended Fluorescent Luminaires | Department

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Energy Products & Technologies » Energy-Efficient Products » Purchasing Energy-Efficient Suspended Fluorescent Luminaires Purchasing Energy-Efficient Suspended Fluorescent Luminaires The Federal Energy Management Program (FEMP) provides acquisition guidance for suspended fluorescent luminaires, a product category covered by FEMP efficiency requirements. Federal laws and requirements mandate that agencies purchase ENERGY STAR-qualified or FEMP-designated products in all product

  4. Yellow fluorescent protein phiYFPv (Phialidium): structure and structure-based mutagenesis

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z. Souslova, Ekaterina; Chudakov, Dmitry M.; Lukyanov, Sergey; Martynov, Vladimir I.; Arhipova, Svetlena; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei

    2013-06-01

    The yellow fluorescent protein phiYFPv with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The yellow fluorescent protein phiYFPv (λ{sub em}{sup max} ≃ 537 nm) with improved folding has been developed from the spectrally identical wild-type phiYFP found in the marine jellyfish Phialidium. The latter fluorescent protein is one of only two known cases of naturally occurring proteins that exhibit emission spectra in the yellow–orange range (535–555 nm). Here, the crystal structure of phiYFPv has been determined at 2.05 Å resolution. The ‘yellow’ chromophore formed from the sequence triad Thr65-Tyr66-Gly67 adopts the bicyclic structure typical of fluorophores emitting in the green spectral range. It was demonstrated that perfect antiparallel π-stacking of chromophore Tyr66 and the proximal Tyr203, as well as Val205, facing the chromophore phenolic ring are chiefly responsible for the observed yellow emission of phiYFPv at 537 nm. Structure-based site-directed mutagenesis has been used to identify the key functional residues in the chromophore environment. The obtained results have been utilized to improve the properties of phiYFPv and its homologous monomeric biomarker tagYFP.

  5. CMI-funded research creates better fluorescent lighting | The...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CMI-funded research creates better fluorescent lighting General Electric (GE), Lawrence Livermore National Laboratory (LLNL) and Oak Ridge National Laboratory (ORNL) have created...

  6. Site-Specific Synthesis and In Situ Immobilization of Fluorescent...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Site-Specific Synthesis and In Situ Immobilization of Fluorescent Silver Nanoclusters on DNA Nanoscaffolds by Use of the Tollens Reaction Authors: Pal, S., Varghese, R., Deng, Z.,...

  7. Stable and responsive fluorescent carbon nanotube silica gels...

    Office of Scientific and Technical Information (OSTI)

    silica nanocomposite gels doped with fluorescent single walled carbon nanotubes (SWNT). ... of highly luminescent SWNTsilica composite materials that are potentially useful ...

  8. Simultaneous cryo X-ray ptychographic and fluorescence microscopy...

    Office of Scientific and Technical Information (OSTI)

    and fluorescence microscopy of green algae Citation Details In-Document Search ... Visit OSTI to utilize additional information resources in energy science and technology. A ...

  9. Better Fluorescent Lighting Through Physics | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    National Labs are working with industry to make fluorescent lighting more environmentally friendly by reducing the need for rare earth elements. | iStock photo. National Labs are ...

  10. Simultaneous cryo X-ray ptychographic and fluorescence microscopy...

    Office of Scientific and Technical Information (OSTI)

    ptychographic and fluorescence microscopy of green algae You are accessing a document from the Department of Energy's (DOE) Public Access Gateway for Energy & Science (PAGES). ...

  11. Fluorescent tracking of nickel ions in human cultured cells ...

    Office of Scientific and Technical Information (OSTI)

    Fluorescent tracking of nickel ions in human cultured cells Citation Details In-Document ... B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International ...

  12. Time-resolved fluorescence decay measurements for flowing particles

    DOE Patents [OSTI]

    Deka, Chiranjit; Steinkamp, John A.

    1999-01-01

    Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.

  13. Time-resolved fluorescence decay measurements for flowing particles

    DOE Patents [OSTI]

    Deka, C.; Steinkamp, J.A.

    1999-06-01

    Time-resolved fluorescence decay measurements are disclosed for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated CW laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes. 12 figs.

  14. Mapping protein collapse with single molecule fluorescence and...

    Office of Scientific and Technical Information (OSTI)

    spectroscopy Citation Details In-Document Search Title: Mapping protein collapse with single molecule fluorescence and kinetic synchrotron radiation circular dichroism spectroscopy ...

  15. Max Tech and Beyond: Fluorescent Lamps

    SciTech Connect (OSTI)

    Scholand, Michael

    2012-04-01

    Fluorescent lamps are the most widely used artificial light source today, responsible for approximately 70% of the lumens delivered to our living spaces globally. The technology was originally commercialized in the 1930's, and manufacturers have been steadily improving the efficacy of these lamps over the years through modifications to the phosphors, cathodes, fill-gas, operating frequency, tube diameter and other design attributes. The most efficient commercially available fluorescent lamp is the 25 Watt T5 lamp. This lamp operates at 114-116 lumens per watt while also providing good color rendering and more than 20,000 hours of operating life. Industry experts interviewed indicated that while this lamp is the most efficient in the market today, there is still a further 10 to 14% of potential improvements that may be introduced to the market over the next 2 to 5 years. These improvements include further developments in phosphors, fill-gas, cathode coatings and ultraviolet (UV) reflective glass coatings. The commercialization of these technology improvements will combine to bring about efficacy improvements that will push the technology up to a maximum 125 to 130 lumens per watt. One critical issue raised by researchers that may present a barrier to the realization of these improvements is the fact that technology investment in fluorescent lamps is being reduced in order to prioritize research into light emitting diodes (LEDs) and ceramic metal halide high intensity discharge (HID) lamps. Thus, it is uncertain whether these potential efficacy improvements will be developed, patented and commercialized. The emphasis for premium efficacy will continue to focus on T5 lamps, which are expected to continue to be marketed along with the T8 lamp. Industry experts highlighted the fact that an advantage of the T5 lamp is the fact that it is 40% smaller and yet provides an equivalent lumen output to that of a T8 or T12 lamp. Due to its smaller form factor, the T5 lamp

  16. Radioiodine detector based on laser induced fluorescence

    DOE Patents [OSTI]

    McDonald, Jimmie R.; Baronavski, Andrew P.

    1980-01-01

    The invention involves the measurement of the concentration of the radioisotope .sup.129 I.sub.2 in the presence of a gas. The invention uses a laser to excite a sample of the .sup.129 I.sub.2 in a sample gas chamber and a reference sample of a known concentration of .sup.129 I.sub.2 in a reference gas chamber. The .sup.129 I.sub.2 in the sample and reference gas chamber each gives off fluorescence emissions which are received by photomultipliers which provide signals to a detector. The detector uses a ratioing technique to determine the concentration of .sup.129 I.sub.2 in the sample gas chamber.

  17. Nuclear Resonance Fluorescence for Materials Assay

    SciTech Connect (OSTI)

    Quiter, Brian J.; Ludewigt, Bernhard; Mozin, Vladimir; Prussin, Stanley

    2009-06-29

    This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has ben performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX?s photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

  18. Nuclear Resonance Fluorescence for Materials Assay

    SciTech Connect (OSTI)

    Quiter, Brian; Ludewigt, Bernhard; Mozin, Vladimir; Prussin, Stanley

    2009-06-05

    This paper discusses the use of nuclear resonance fluorescence (NRF) techniques for the isotopic and quantitative assaying of radioactive material. Potential applications include age-dating of an unknown radioactive source, pre- and post-detonation nuclear forensics, and safeguards for nuclear fuel cycles Examples of age-dating a strong radioactive source and assaying a spent fuel pin are discussed. The modeling work has ben performed with the Monte Carlo radiation transport computer code MCNPX, and the capability to simulate NRF has bee added to the code. Discussed are the limitations in MCNPX's photon transport physics for accurately describing photon scattering processes that are important contributions to the background and impact the applicability of the NRF assay technique.

  19. Untitled

    U.S. Energy Information Administration (EIA) Indexed Site

    descriptions of the amount of electricity used for lighting and variations in households' consumption of electricity for lighting. Types of Lights Dominance of Incandescent Lights...

  20. Optical Control of Fluorescence through plasmonic eigenmode extinction

    SciTech Connect (OSTI)

    Xu, Xiaoying; Lin, Shih-Che; Li, Quanshui; Zhang, Zhili; Ivanov, Ilia N.; Li, Yuan; Wang, Wenbin; Gu, Baohua; Zhang, Zhenyu; Hsueh, C. H.; Snijders, Paul C.; Seal, Katyayani

    2015-04-30

    We introduce the concept of optical control of the fluorescence yield of CdSe quantum dots through plasmon-induced structural changes in random semicontinuous nanostructured gold films. We demonstrate that the wavelength- and polarization dependent coupling between quantum dots and the semicontinuous films, and thus the fluorescent emission spectrum, can be controlled and significantly increased through the optical extinction of a selective band of eigenmodes in the films. This optical method of effecting controlled changes in the metal nanostructure allows for versatile functionality in a single sample and opens a pathway to in situ control over the fluorescence spectrum.

  1. Fluorescent lamp unit with magnetic field generating means

    DOE Patents [OSTI]

    Grossman, Mark W.; George, William A.

    1989-01-01

    A fluorescent lamp unit having a magnetic field generating means for improving the performance of the fluorescent lamp is disclosed. In a preferred embodiment the fluorescent lamp comprises four longitudinally extending leg portions disposed in substantially quadrangular columnar array and joined by three generally U-shaped portions disposed in different planes. In another embodiment of the invention the magnetic field generating means comprises a plurality of permanent magnets secured together to form a single columnar structure disposed within a centrally located region defined by the shape of lamp envelope.

  2. Fluorescent lamp unit with magnetic field generating means

    DOE Patents [OSTI]

    Grossman, M.W.; George, W.A.

    1989-08-08

    A fluorescent lamp unit having a magnetic field generating means for improving the performance of the fluorescent lamp is disclosed. In a preferred embodiment the fluorescent lamp comprises four longitudinally extending leg portions disposed in substantially quadrangular columnar array and joined by three generally U-shaped portions disposed in different planes. In another embodiment of the invention the magnetic field generating means comprises a plurality of permanent magnets secured together to form a single columnar structure disposed within a centrally located region defined by the shape of lamp envelope. 4 figs.

  3. Ultrahigh resolution multicolor colocalization of single fluorescent probes

    DOE Patents [OSTI]

    Weiss, Shimon; Michalet, Xavier; Lacoste, Thilo D.

    2005-01-18

    A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.

  4. System and method for measuring fluorescence of a sample

    DOE Patents [OSTI]

    Riot, Vincent J

    2015-03-24

    The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage.

  5. Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

    SciTech Connect (OSTI)

    Bernassau, A. L.; Al-Rawhani, M.; Beeley, J.; Cumming, D. R. S. [School of Engineering, University of Glasgow, Glasgow, G12 8LT (United Kingdom)] [School of Engineering, University of Glasgow, Glasgow, G12 8LT (United Kingdom)

    2013-12-09

    A compact hybrid system has been developed to position and detect fluorescent micro-particles by combining a Single Photon Avalanche Diode (SPAD) imager with an acoustic manipulator. The detector comprises a SPAD array, light-emitting diode (LED), lenses, and optical filters. The acoustic device is formed of multiple transducers surrounding an octagonal cavity. By stimulating pairs of transducers simultaneously, an acoustic landscape is created causing fluorescent micro-particles to agglomerate into lines. The fluorescent pattern is excited by a low power LED and detected by the SPAD imager. Our technique combines particle manipulation and visualization in a compact, low power, portable setup.

  6. Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore

    SciTech Connect (OSTI)

    Pletneva, Nadya V.; Pletnev, Vladimir Z.; Shemiakina, Irina I.; Chudakov, Dmitriy M.; Artemyev, Igor; Wlodawer, Alexander; Dauter, Zbigniew; Pletnev, Sergei

    2012-08-10

    The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, {lambda}{sub ex} = 552 nm, {lambda}{sub em} = 578 nm) and its bright far-red fluorescent variant Katushka ({lambda}{sub ex} = 588 nm, {lambda}{sub em} = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 {angstrom} at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f.

  7. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Dougalas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2003-12-09

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  8. Protein subcellular localization assays using split fluorescent proteins

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2009-09-08

    The invention provides protein subcellular localization assays using split fluorescent protein systems. The assays are conducted in living cells, do not require fixation and washing steps inherent in existing immunostaining and related techniques, and permit rapid, non-invasive, direct visualization of protein localization in living cells. The split fluorescent protein systems used in the practice of the invention generally comprise two or more self-complementing fragments of a fluorescent protein, such as GFP, wherein one or more of the fragments correspond to one or more beta-strand microdomains and are used to "tag" proteins of interest, and a complementary "assay" fragment of the fluorescent protein. Either or both of the fragments may be functionalized with a subcellular targeting sequence enabling it to be expressed in or directed to a particular subcellular compartment (i.e., the nucleus).

  9. Calibration of fluorescence resonance energy transfer in microscopy

    DOE Patents [OSTI]

    Youvan, Douglas C.; Silva, Christopher M.; Bylina, Edward J.; Coleman, William J.; Dilworth, Michael R.; Yang, Mary M.

    2002-09-24

    Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

  10. Method and apparatus for detection of fluorescently labeled materials

    DOE Patents [OSTI]

    Stern, David; Fiekowsky, Peter

    2004-05-25

    Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

  11. Assessment of Nuclear Resonance Fluorescence for Spent Nuclear Fuel Assay

    SciTech Connect (OSTI)

    Quiter, Brian; Ludewigt, Bernhard; Ambers, Scott

    2011-06-30

    In nuclear resonance fluorescence (NRF) measurements, resonances are excited by an external photon beam leading to the emission of gamma rays with specific energies that are characteristic of the emitting isotope. NRF promises the unique capability of directly quantifying a specific isotope without the need for unfolding the combined responses of several fissile isotopes as is required in other measurement techniques. We have analyzed the potential of NRF as a non-destructive analysis technique for quantitative measurements of Pu isotopes in spent nuclear fuel (SNF). Given the low concentrations of 239Pu in SNF and its small integrated NRF cross sections, the main challenge in achieving precise and accurate measurements lies in accruing sufficient counting statistics in a reasonable measurement time. Using analytical modeling, and simulations with the radiation transport code MCNPX that has been experimentally tested recently, the backscatter and transmission methods were quantitatively studied for differing photon sources and radiation detector types. Resonant photon count rates and measurement times were estimated for a range of photon source and detection parameters, which were used to determine photon source and gamma-ray detector requirements. The results indicate that systems based on a bremsstrahlung source and present detector technology are not practical for high-precision measurements of 239Pu in SNF. Measurements that achieve the desired uncertainties within hour-long measurements will either require stronger resonances, which may be expressed by other Pu isotopes, or require quasi-monoenergetic photon sources with intensities that are approximately two orders of magnitude higher than those currently being designed or proposed.This work is part of a larger effort sponsored by the Next Generation Safeguards Initiative to develop an integrated instrument, comprised of individual NDA techniques with complementary features, that is fully capable of

  12. Life-Cycle Assessment of Energy and Environmental Impacts of...

    Energy Savers [EERE]

    Life-Cycle Assessment of Energy and Environmental Impacts of LED Lighting Products Part I: Review of the Life-Cycle Energy Consumption of Incandescent, Compact Fluorescent, and LED ...

  13. Energy Savings Forecast of Solid-State Lighting in General Illumination Applications

    Broader source: Energy.gov [DOE]

    Report forecasting the U.S. energy savings of LED white-light sources compared to conventional white-light sources (i.e., incandescent, halogen, fluorescent, and high-intensity discharge) over the...

  14. School Energy Survey Student Guide

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of 40 new power plants. INCANDESCENT BULB HALOGEN COMPACT FLUORESCENT (CFL) LIGHT EMITTING DIODE (LED) Brightness 850 lumens 850 lumens 850 lumens 850 lumens Life of Bulb 1,000...

  15. School Energy Survey Teacher Guide

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Key Student Guide Page 20 INCANDESCENT BULB HALOGEN COMPACT FLUORESCENT (CFL) LIGHT EMITTING DIODE (LED) Number of bulbs to get 25,000 hours 25 8.3 2.5 1 Cost of bulbs for 25,000...

  16. LucY: A versatile new fluorescent reporter protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Auldridge, Michele E.; Cao, Hongnan; Sen, Saurabh; Franz, Laura P.; Bingman, Craig A.; Yennamalli, Ragothaman M.; Phillips, Jr., George N.; Mead, David; Steinmetz, Eric J.; Michnick, Stephen W.

    2015-04-23

    We report on the discovery, isolation, and use of a novel yellow fluorescent protein. Lucigen Yellow (LucY) binds one FAD molecule within its core, thus shielding it from water and maintaining its structure so that fluorescence is 10-fold higher than freely soluble FAD. LucY displays excitation and emission spectra characteristic of FAD, with 3 excitation peaks at 276nm, 377nm, and 460nm and a single emission peak at 530nm. These excitation and emission maxima provide the large Stokes shift beneficial to fluorescence experimentation. LucY belongs to the MurB family of UDP-N-acetylenolpyruvylglucosamine reductases. The high resolution crystal structure shows that in contrastmore » to other structurally resolved MurB enzymes, LucY does not contain a potentially quenching aromatic residue near the FAD isoalloxazine ring, which may explain its increased fluorescence over related proteins. Using E. coli as a system in which to develop LucY as a reporter, we show that it is amenable to circular permutation and use as a reporter of protein-protein interaction. Fragmentation between its distinct domains renders LucY non-fluorescent, but fluorescence can be partially restored by fusion of the fragments to interacting protein domains. Thus, LucY may find application in Protein-fragment Complementation Assays for evaluating protein-protein interactions.« less

  17. Rigidifying Fluorescent Linkers by Metal-Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement

    SciTech Connect (OSTI)

    Wei, ZW; Gu, ZY; Arvapally, RK; Chen, YP; McDougald, RN; Ivy, JF; Yakovenko, AA; Feng, DW; Omary, MA; Zhou, HC

    2014-06-11

    We demonstrate that rigidifying the structure of fluorescent linkers by structurally constraining them in metal-organic frameworks (MOFs) to control their conformation effectively tunes the fluorescence energy and enhances the quantum yield. Thus, a new tetraphenylethylene-based zirconium MOF exhibits a deep-blue fluorescent emission at 470 nm with a unity quantum yield (99.9 +/- 0.5%) under Ar, representing ca. 3600 cm(-1) blue shift and doubled radiative decay efficiency vs the linker precursor. An anomalous increase in the fluorescence lifetime and relative intensity takes place upon heating the solid MOF from cryogenic to ambient temperatures. The origin of these unusual photoluminescence properties is attributed to twisted linker conformation, intramolecular hindrance, and framework rigidity.

  18. Rigidifying Fluorescent Linkers by Metal–Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement

    SciTech Connect (OSTI)

    Wei, Zhangwen; Gu, Zhi-Yuan; Arvapally, Ravi K.; Chen, Ying-Pin; Ivy, Joshua F.; Yakovenko, Andrey A.; Feng, Dawei; Omary, Mohammad A.; Zhou, Hong-Cai

    2014-06-11

    We demonstrate that rigidifying the structure of fluorescent linkers by structurally constraining them in metal–organic frameworks (MOFs) to control their conformation effectively tunes the fluorescence energy and enhances the quantum yield. Thus, a new tetraphenylethylene-based zirconium MOF exhibits a deep-blue fluorescent emission at 470 nm with a unity quantum yield (99.9 ± 0.5%) under Ar, representing ca. 3600 cm⁻¹ blue shift and doubled radiative decay efficiency vs the linker precursor. An anomalous increase in the fluorescence lifetime and relative intensity takes place upon heating the solid MOF from cryogenic to ambient temperatures. The origin of these unusual photoluminescence properties is attributed to twisted linker conformation, intramolecular hindrance, and framework rigidity.

  19. Technical description of candidate fluorescence compounds and radioisotopes for a nuclear smuggling deterrence tag (IL500E)

    SciTech Connect (OSTI)

    Hartenstein, S.D.; Aryaeinejad, R.

    1996-03-01

    This report summarizes the efforts completed in identifying candidate fluorescence compounds and radioisotopes for a developing tagging system. The tagging system is being developed as a deterrent to nuclear smuggling, by providing a means of: (1) tracing materials and pilferers to the facility of origin for any recovered special nuclear materials; (2) inventory control of long-term stored items containing special nuclear materials; and (3) tracking materials transferred between facilities. The tagging system uses four types of tagging materials to cover a range of applications intended to prevent the pilfering of special nuclear materials. One material, fluorescent compounds which are invisible without ultraviolet or near-infrared detection systems, is marked on controlled items with a tracking pattern that corresponds to a specified item in a specified location in the data control system. The tagging system uses an invisible, fluorescent dusting powder to mark equipment and personnel who inappropriately handle the tagged material. The tagging system also uses unique combinations of radionuclides to identify the facility of origin for any special nuclear material. Currently, 18 long-lived radioisotopes, 38 short-live radioisotopes and 10 fluorescent compounds have been selected as candidate materials for the tagging system.

  20. Total reflection x-ray fluorescence: Determination of an optimum geometry

    SciTech Connect (OSTI)

    Koo, Y.M.; Chang, C.H.; Padmore, H.A.

    1997-04-01

    Total reflection X-Ray Fluorescence (TXRF) is a widely used technique in which the normal trace element detection capability of hard x-ray fluorescence (XRF) is enhanced by use of an x-ray reflective substrate. TXRF is more sensitive than normal photon induced XRF due to the reduction of the substrate scattering and fluorescence signals. This reduction comes about because in total external reflection, the photon field only penetrates about 20 {angstrom} into the surface, instead of typically 50 {mu}m for a silicon substrate at normal incidence for 10 KeV photons. The technique is used in many fields of trace element analysis, and is widely used in the determination of metal impurity concentrations on and in the surface of silicon wafers. The Semiconductor Industry Association roadmap (SIA) indicates a need for wafer contamination detection at the 10{sup 7}atoms/cm{sup 2} level in the next few years. Current commercial systems using rotating anode x-ray sources presently routinely operate with a sensitivity level of around 10{sup 10} atoms/cm{sup 2} and this has led to interest in the use of synchrotron radiation to extend the sensitivity by three orders of magnitude. The pioneering work of Pianetta and co-workers at SSRL has clearly shown that this should be possible, using a fully optimized source and detector. The purpose of this work is to determine whether ALS would be a suitable source for this type of highly sensitive wafer TXRF. At first look it appears improbable as the SSRL work used a high flux multipole wiggler source, and it is clear that the detected fluorescence for relevant concentrations is small. In addition, SSRL operates at 3.0 GeV rather than 1.9 GeV, and is therefore more naturally suited to hard x-ray experiments. The aim of this work was therefore to establish a theoretical model for the scattering and fluorescence processes, so that one could predict the differences between alternative geometries and select an optimum configuration.

  1. Study: Environmental Benefits of LEDs Greater Than CFLs | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Study: Environmental Benefits of LEDs Greater Than CFLs Study: Environmental Benefits of LEDs Greater Than CFLs December 9, 2013 - 4:13pm Addthis A three-part Energy Department-funded study indicates LEDs are more environmentally friendly than compact fluorescent and incandescent lights. | Energy Department graphic A three-part Energy Department-funded study indicates LEDs are more environmentally friendly than compact fluorescent and incandescent lights. | Energy Department graphic

  2. Cyanine-based probe\\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

    DOE Patents [OSTI]

    Mayer-Cumblidge, M. Uljana; Cao, Haishi

    2013-01-15

    A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

  3. ARM - Measurement - Cloud type

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Measurement : Cloud type Cloud type such as cirrus, stratus, cumulus etc Categories Cloud Properties Instruments The above measurement is considered scientifically relevant for the...

  4. Nuclear Resonance Fluorescence for Safeguards Applications

    SciTech Connect (OSTI)

    Ludewigt, Bernhard A; Quiter, Brian J; Ambers, Scott D

    2011-02-04

    In nuclear resonance fluorescence (NRF) measurements, resonances are excited by an external photon beam leading to the emission of {gamma} rays with specific energies that are characteristic of the emitting isotope. The promise of NRF as a non-destructive analysis technique (NDA) in safeguards applications lies in its potential to directly quantify a specific isotope in an assay target without the need for unfolding the combined responses of several fissile isotopes as often required by other NDA methods. The use of NRF for detection of sensitive nuclear materials and other contraband has been researched in the past. In the safeguards applications considered here one has to go beyond mere detection and precisely quantify the isotopic content, a challenge that is discussed throughout this report. Basic NRF measurement methods, instrumentation, and the analytical calculation of NRF signal strengths are described in Section 2. Well understood modeling and simulation tools are needed for assessing the potential of NRF for safeguards and for designing measurement systems. All our simulations were performed with the radiation transport code MCNPX, a code that is widely used in the safeguards community. Our initial studies showed that MCNPX grossly underestimated the elastically scattered background at backwards angles due to an incorrect treatment of Rayleigh scattering. While new, corrected calculations based on ENDF form factors showed much better agreement with experimental data for the elastic scattering of photons on an uranium target, the elastic backscatter is still not rigorously treated. Photonuclear scattering processes (nuclear Thomson, Delbruck and Giant Dipole Resonance scattering), which are expected to play an important role at higher energies, are not yet included. These missing elastic scattering contributions were studied and their importance evaluated evaluated against data found in the literature as discussed in Section 3. A transmission experiment

  5. Fluorescent single walled nanotube/silica composite materials

    DOE Patents [OSTI]

    Dattelbaum, Andrew M.; Gupta, Gautam; Duque, Juan G.; Doorn, Stephen K.; Hamilton, Christopher E.; DeFriend Obrey, Kimberly A.

    2013-03-12

    Fluorescent composites of surfactant-wrapped single-walled carbon nanotubes (SWNTs) were prepared by exposing suspensions of surfactant-wrapped carbon nanotubes to tetramethylorthosilicate (TMOS) vapor. Sodium deoxycholate (DOC) and sodium dodecylsulphate (SDS) were the surfactants. No loss in emission intensity was observed when the suspension of DOC-wrapped SWNTs were exposed to the TMOS vapors, but about a 50% decrease in the emission signal was observed from the SDS-wrapped SWNTs nanotubes. The decrease in emission was minimal by buffering the SDS/SWNT suspension prior to forming the composite. Fluorescent xerogels were prepared by adding glycerol to the SWNT suspensions prior to TMOS vapor exposure, followed by drying the gels. Fluorescent aerogels were prepared by replacing water in the gels with methanol and then exposing them to supercritical fluid drying conditions. The aerogels can be used for gas sensing.

  6. High lumen compact fluorescents boost light output in new fixtures

    SciTech Connect (OSTI)

    1992-12-31

    Some compact fluorescent lamps aren`t so compact. General Electric (GE), OSRAM, and Philips have been expanding offerings in longer, more powerful, hard wired CFLs that generate enough light to serve applications once limited to conventional fluorescents and metal halide systems. All three of these manufacturers have for some time offered 18- to 40-watt high-output CFLs, which use a fluorescent tube doubled back on itself to produce a lot of light in a compact source. Now GE has introduced an even larger, more powerful 50-watt unit, and OSRAM is soon to follow suit with a 55-watt lamp. These new entries to the field of turbocharged CFLs can provide general lighting at ceiling heights of 12 feet or more as well as indirect lighting, floodlighting, and wall washing. They are such a concentrated source of light that they can provide the desired illumination using fewer lamps and fixtures than would be needed with competing sources.

  7. Laser-induced differential normalized fluorescence method for cancer diagnosis

    DOE Patents [OSTI]

    Vo-Dinh, Tuan; Panjehpour, Masoud; Overholt, Bergein F.

    1996-01-01

    An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample.

  8. Laser-induced differential normalized fluorescence method for cancer diagnosis

    DOE Patents [OSTI]

    Vo-Dinh, T.; Panjehpour, M.; Overholt, B.F.

    1996-12-03

    An apparatus and method for cancer diagnosis are disclosed. The diagnostic method includes the steps of irradiating a tissue sample with monochromatic excitation light, producing a laser-induced fluorescence spectrum from emission radiation generated by interaction of the excitation light with the tissue sample, and dividing the intensity at each wavelength of the laser-induced fluorescence spectrum by the integrated area under the laser-induced fluorescence spectrum to produce a normalized spectrum. A mathematical difference between the normalized spectrum and an average value of a reference set of normalized spectra which correspond to normal tissues is calculated, which provides for amplifying small changes in weak signals from malignant tissues for improved analysis. The calculated differential normalized spectrum is correlated to a specific condition of a tissue sample. 5 figs.

  9. Biochip Image Grid Normalization Absolute Signal Fluorescence Measurement Using

    Energy Science and Technology Software Center (OSTI)

    2001-04-17

    This software was developed to measure absolute fluorescent intensities of gel pads on a microchip in units defined by a standard fluorescent slide. It can accomodate varying measurement conditions (e.g. exposure time, sensitivity of detector, resolution of detector, etc.) as well as fluorescent microscopes with non-uniform sensitivity across their field of view allowing the user to compare measurements done on different detectors with varying exposure times, sensitivities, and resolutions. The software is designed both tomore » operate Roper Scientific, Inc. cameras and to use image files produced by the program supplied with that equipment for its calculations. the intensity of the gel pad signal is computed so as to reduce background influence.« less

  10. Engineering and Characterization of a Superfolder Green Fluorescent Protein

    SciTech Connect (OSTI)

    Pedelacq,J.; Cabantous, S.; Tran, T.; Terwilliger, T.; Waldo, G.

    2006-01-01

    Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.

  11. Fluorescence spectra and biological activity of aerosolized bacillus spores and MS2 bacteriophage exposed to ozone at different relative humidities in a rotating drum

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ratnesar-Shumate, Shanna; Pan, Yong-Le; Hill, Steven C.; Kinahan, Sean; Corson, Elizabeth; Eshbaugh, Jonathan; Santarpia, Joshua L.

    2015-10-14

    Biological aerosols (bioaerosols) released into the environment may undergo physical and chemical transformations when exposed to atmospheric constituents such as solar irradiation, reactive oxygenated species, ozone, free radicals, water vapor and pollutants. Aging experiments were performed in a rotating drum chamber subjecting bioaerosols, Bacillus thuringiensis Al Hakam (BtAH) spores and MS2 bacteriophages to ozone at 0 and 150 ppb, and relative humidities (RH) at 10%, 50%, and 80+%. Fluorescence spectra and intensities of the aerosols as a function of time in the reaction chamber were measured with a single particle fluorescence spectrometer (SPFS) and an Ultra-Violet Aerodynamic Particle Sizer® Spectrometermore » (UV-APS). Losses in biological activity were measured by culture and quantitative polymerase chain reaction (q-PCR) assay. For both types of aerosols the largest change in fluorescence emission was between 280 and 400 nm when excited at 263 nm followed by fluorescence emission between 380 and 700 nm when excited at 351 nm. The fluorescence for both BtAH and MS2 were observed to decrease significantly at high ozone concentration and high RH when excited at 263 nm excitation. The decreases in 263 nm excited fluorescence are indicative of hydrolysis and oxidation of tryptophan in the aerosols. Fluorescence measured with the UV-APS (355-nm excitation) increased with time for both BtAH and MS2 aerosols. A two log loss of MS2 bacteriophage infectivity was observed in the presence of ozone at ~50% and 80% RH when measured by culture and normalized for physical losses by q-PCR. Viability of BtAH spores after exposure could not be measured due to the loss of genomic material during experiments, suggesting degradation of extracelluar DNA attributable to oxidation. The results of these studies indicate that the physical and biological properties of bioaerosols change significantly after exposure to ozone and water vapor.« less

  12. Fluorescence spectra and biological activity of aerosolized bacillus spores and MS2 bacteriophage exposed to ozone at different relative humidities in a rotating drum

    SciTech Connect (OSTI)

    Ratnesar-Shumate, Shanna; Pan, Yong-Le; Hill, Steven C.; Kinahan, Sean; Corson, Elizabeth; Eshbaugh, Jonathan; Santarpia, Joshua L.

    2015-10-14

    Biological aerosols (bioaerosols) released into the environment may undergo physical and chemical transformations when exposed to atmospheric constituents such as solar irradiation, reactive oxygenated species, ozone, free radicals, water vapor and pollutants. Aging experiments were performed in a rotating drum chamber subjecting bioaerosols, Bacillus thuringiensis Al Hakam (BtAH) spores and MS2 bacteriophages to ozone at 0 and 150 ppb, and relative humidities (RH) at 10%, 50%, and 80+%. Fluorescence spectra and intensities of the aerosols as a function of time in the reaction chamber were measured with a single particle fluorescence spectrometer (SPFS) and an Ultra-Violet Aerodynamic Particle Sizer® Spectrometer (UV-APS). Losses in biological activity were measured by culture and quantitative polymerase chain reaction (q-PCR) assay. For both types of aerosols the largest change in fluorescence emission was between 280 and 400 nm when excited at 263 nm followed by fluorescence emission between 380 and 700 nm when excited at 351 nm. The fluorescence for both BtAH and MS2 were observed to decrease significantly at high ozone concentration and high RH when excited at 263 nm excitation. The decreases in 263 nm excited fluorescence are indicative of hydrolysis and oxidation of tryptophan in the aerosols. Fluorescence measured with the UV-APS (355-nm excitation) increased with time for both BtAH and MS2 aerosols. A two log loss of MS2 bacteriophage infectivity was observed in the presence of ozone at ~50% and 80% RH when measured by culture and normalized for physical losses by q-PCR. Viability of BtAH spores after exposure could not be measured due to the loss of genomic material during experiments, suggesting degradation of extracelluar DNA attributable to oxidation. The results of these studies indicate that the physical and biological properties of bioaerosols change significantly after exposure to ozone and water vapor.

  13. Heat transfer assembly for a fluorescent lamp and fixture

    DOE Patents [OSTI]

    Siminovitch, M.J.; Rubenstein, F.M.; Whitman, R.E.

    1992-12-29

    In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure. 11 figs.

  14. Heat transfer assembly for a fluorescent lamp and fixture

    DOE Patents [OSTI]

    Siminovitch, Michael J.; Rubenstein, Francis M.; Whitman, Richard E.

    1992-01-01

    In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure.

  15. Fluorescence dye tagging scheme for mercury quantification and speciation

    DOE Patents [OSTI]

    Jiao, Hong; Catterall, Hannah

    2015-09-22

    A fluorescent dye or fluorophore capable of forming complexes with mercury comprises 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate amide, wherein the amide is formed by reacting the succinimidyl ester (Pacific Blue.TM.) with an amino acid containing a thiol group, such as cysteine or glutathione. Mercury complexes of the fluorophore fluoresce when excited by a UV or violet laser diode, and the detected intensity can be calibrated to quantify the concentration of mercury in a sample reacted with the fluorophore.

  16. Digital optical phase conjugation of fluorescence in turbid tissue

    SciTech Connect (OSTI)

    Vellekoop, Ivo M.; Cui Meng; Yang Changhuei

    2012-08-20

    We demonstrate a method for phase conjugating fluorescence. Our method, called reference free digital optical phase conjugation, can conjugate extremely weak, incoherent optical signals. It was used to phase conjugate fluorescent light originating from a bead covered with 0.5 mm of light-scattering tissue. The phase conjugated beam refocuses onto the bead and causes a local increase of over two orders of magnitude in the light intensity. Potential applications are in imaging, optical trapping, and targeted photochemical activation inside turbid tissue.

  17. Synchrotron Radiation {mu}-X Ray Fluorescence on Multicellular Tumor Spheroids

    SciTech Connect (OSTI)

    Burattini, E.; Cinque, G.; Bellisola, G.; Fracasso, G.; Colombatti, M.; Monti, F.

    2003-01-24

    Synchrotron Radiation micro X-Ray Fluorescence (SR {mu}-XRF) was applied for the first time to map the trace element content on Multicellular Tumor Spheroids (MTS), i.e. human cell clusters used as an in vitro model for testing micrometastases responses to antitumoral drugs. In particular, immunotoxin molecules composed of a carrier protein (Transferrin) bound to a powerful cytotoxin (Ricin A), were here considered as representatives of a class of therapheutic macromolecules used in cancer theraphy. Spheroids included in polyacrylamide gel and placed inside quartz capillaries were studied at the ESRF ID22 beamline using a 15 keV monochromatic photon microbeam. Elemental maps (of Fe, Cu, Zn and Pb) on four groups of spheroids grown under different conditions were studied: untreated, treated only with the carrier molecule or with the toxin alone, and with the complete immunotoxin molecule (carrier+toxin). The results indicate that the distribution of Zn and, to some extent, Cu in the spheroid cells is homogeneous and independent of the treatment type. Total Reflection X-Ray Fluorescence (TR-XRF) was also applied to quantify the average trace element content in the spheroids. Future developments of the technique are finally outlined on the basis of these preliminary results.

  18. 3D optical sectioning with a new hyperspectral confocal fluorescence imaging system.

    SciTech Connect (OSTI)

    Nieman, Linda T.; Sinclair, Michael B.; Davidson, George S.; Van Benthem, Mark Hilary; Haaland, David Michael; Timlin, Jerilyn Ann; Sasaki, Darryl Yoshio; Bachand, George David; Jones, Howland D. T.

    2007-02-01

    A novel hyperspectral fluorescence microscope for high-resolution 3D optical sectioning of cells and other structures has been designed, constructed, and used to investigate a number of different problems. We have significantly extended new multivariate curve resolution (MCR) data analysis methods to deconvolve the hyperspectral image data and to rapidly extract quantitative 3D concentration distribution maps of all emitting species. The imaging system has many advantages over current confocal imaging systems including simultaneous monitoring of numerous highly overlapped fluorophores, immunity to autofluorescence or impurity fluorescence, enhanced sensitivity, and dramatically improved accuracy, reliability, and dynamic range. Efficient data compression in the spectral dimension has allowed personal computers to perform quantitative analysis of hyperspectral images of large size without loss of image quality. We have also developed and tested software to perform analysis of time resolved hyperspectral images using trilinear multivariate analysis methods. The new imaging system is an enabling technology for numerous applications including (1) 3D composition mapping analysis of multicomponent processes occurring during host-pathogen interactions, (2) monitoring microfluidic processes, (3) imaging of molecular motors and (4) understanding photosynthetic processes in wild type and mutant Synechocystis cyanobacteria.

  19. Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

    DOE Patents [OSTI]

    Weiss, R.B.; Kimball, A.W.; Gesteland, R.F.; Ferguson, F.M.; Dunn, D.M.; Di Sera, L.J.; Cherry, J.L.

    1995-11-28

    A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, the enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots. 9 figs.

  20. Automated hybridization/imaging device for fluorescent multiplex DNA sequencing

    DOE Patents [OSTI]

    Weiss, Robert B.; Kimball, Alvin W.; Gesteland, Raymond F.; Ferguson, F. Mark; Dunn, Diane M.; Di Sera, Leonard J.; Cherry, Joshua L.

    1995-01-01

    A method is disclosed for automated multiplex sequencing of DNA with an integrated automated imaging hybridization chamber system. This system comprises an hybridization chamber device for mounting a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber device, imaging apparatus for light delivery to the membrane and image recording of fluorescence emanating from the membrane while in the chamber device, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme (such as alkaline phosphatase) is bound to a binding moiety on the probe, and a fluorogenic substrate (such as a benzothiazole derivative) is introduced into the chamber device by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber device with a beam of light from the imaging apparatus, excites fluorescence of the fluorescent product to produce a pattern of hybridization. The pattern of hybridization is imaged by a CCD camera component of the imaging apparatus to obtain a series of digital signals. These signals are converted by the controller apparatus into a string of nucleotides corresponding to the nucleotide sequence an automated sequence reader. The method and apparatus are also applicable to other membrane-based applications such as colony and plaque hybridization and Southern, Northern, and Western blots.

  1. Fluorescent lamp with static magnetic field generating means

    DOE Patents [OSTI]

    Moskowitz, P.E.; Maya, J.

    1987-09-08

    A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed. 2 figs.

  2. An Analog Filter Approach to Frequency Domain Fluorescence Spectroscopy

    SciTech Connect (OSTI)

    Trainham, Clifford P.; O'Neill, Mary D.; McKenna, Ian J.

    2015-04-24

    The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence terms can be modeled as circuit components and cascaded with drive and detection electronics to produce a global transfer function. Electronics design tools such as Spicea can be used to model fluorescence problems. In applications, such as remote sensing, where detection electronics are operated at high gain and limited bandwidth, a global modeling of the entire system is important, since the filter terms of the drive and detection electronics affect the measured response of the fluorescence signals. The techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response.

  3. Fluorescence photon migration by the boundary element method

    SciTech Connect (OSTI)

    Fedele, Francesco; Eppstein, Margaret J. . E-mail: maggie.eppstein@uvm.edu; Laible, Jeffrey P.; Godavarty, Anuradha; Sevick-Muraca, Eva M.

    2005-11-20

    The use of the boundary element method (BEM) is explored as an alternative to the finite element method (FEM) solution methodology for the elliptic equations used to model the generation and transport of fluorescent light in highly scattering media, without the need for an internal volume mesh. The method is appropriate for domains where it is reasonable to assume the fluorescent properties are regionally homogeneous, such as when using highly specific molecularly targeted fluorescent contrast agents in biological tissues. In comparison to analytical results on a homogeneous sphere, BEM predictions of complex emission fluence are shown to be more accurate and stable than those of the FEM. Emission fluence predictions made with the BEM using a 708-node mesh, with roughly double the inter-node spacing of boundary nodes as in a 6956-node FEM mesh, match experimental frequency-domain fluorescence emission measurements acquired on a 1087 cm{sup 3} breast-mimicking phantom at least as well as those of the FEM, but require only 1/8 to 1/2 the computation time.

  4. Fluorescent lamp with static magnetic field generating means

    DOE Patents [OSTI]

    Moskowitz, Philip E.; Maya, Jakob

    1987-01-01

    A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed.

  5. An analog filter approach to frequency domain fluorescence spectroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Trainham, Clifford P.; O'Neill, Mary D.; McKenna, Ian J.

    2015-10-01

    The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence terms can be modeled as circuit components and cascaded with drive and detection electronics to produce a global transfer function. Electronics design tools such as Spicea can be used to model fluorescence problems. In applications, such as remote sensing, where detection electronics are operated at high gain and limited bandwidth, a global modeling of the entiremore » system is important, since the filter terms of the drive and detection electronics affect the measured response of the fluorescence signals. Furthermore, the techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response.« less

  6. Types of Reuse

    Broader source: Energy.gov [DOE]

    The following provides greater detail regarding the types of reuse pursued for LM sites. It should be noted that many actual reuses combine several types of the uses listed below.

  7. Types of Radiation Exposure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    External Irradiation Contamination Incorporation Biological Effects of Acute, Total Body Irradiation Managing Radiation Emergencies Procedure Demonstration Types of radiation ...

  8. Postdoc Appointment Types

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Appointment Types Postdoc Appointment Types Most postdocs will be offered a postdoctoral research associate appointment. Each year, approximately 30 Postdoctoral Fellow appointments, including the Distinguished Fellows, are awarded. Postdoc appointment types offer world of possibilities Meet the current LANL Distinguished Postdocs Research Associates Research Associates pursue research as part of ongoing LANL science and engineering programs. Sponsored postdoctoral candidate packages are

  9. Ruby and Sm:YAG fluorescence pressure gauges up to 120 GPa and...

    Office of Scientific and Technical Information (OSTI)

    Ruby and Sm:YAG fluorescence pressure gauges up to 120 GPa and 700 K Citation Details In-Document Search Title: Ruby and Sm:YAG fluorescence pressure gauges up to 120 GPa and 700 K ...

  10. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, Alexander N.; Benson, Scott C.

    1997-01-01

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

  11. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, Alexander M.; Benson, Scott C.

    1999-01-01

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

  12. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, Alexander N.; Benson, Scott C.

    1995-01-01

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

  13. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, A.N.; Benson, S.C.

    1997-07-08

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated. 4 figs.

  14. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, A.M.; Benson, S.C.

    1998-06-16

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated. 4 figs.

  15. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, Alexander M.; Benson, Scott C.

    1998-01-01

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

  16. DNA complexes with dyes designed for energy transfer as fluorescent markers

    DOE Patents [OSTI]

    Glazer, A.N.; Benson, S.C.

    1995-03-28

    Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated. 4 figures.

  17. Exchange of Type II Dockerin-Containing Subunits of the C. thermocellum Cellulosome as Revealed by SNAP-tags

    SciTech Connect (OSTI)

    Waller, Benjamin; Olson, Daniel G.; Currie, Devin; Guss, Adam M; Lynd, Lee R

    2013-01-01

    Clostridium thermocellum is a thermophilic anaerobic bacterium which efficiently hydrolyzes and metabolizes cellulose to ethanol through the action of its cellulosome, a multiprotein enzymatic complex. A fluorescent protein probe, consisting of a type II dockerin module fused to a SNAP-tag, was developed in order to gain insight into the quaternary configuration of the cellulosome and to investigate the effect of deleting cipA, the protein scaffold on which the cellulosome is built. Fluorescence microscopy suggested that the probe had localized to polycellulosomal protuberances on the cell surface. Surprisingly, fluorescence intensity did not substantially change in the cipA deletion mutants. Sequential labeling experiments suggested that this was a result of bound type II dockerins from CipA being replaced by unbound type II dockerins from the fluorophore-SNAP-XDocII probe. This mechanism of dockerin exchange could represent an efficient means for modifying cellulosome composition.

  18. Development of real time detector for fluorescent particles

    SciTech Connect (OSTI)

    Prevost, C.; Vendel, J.; Seigneur, A.

    1997-08-01

    Aerosols tagged by a fluorescent dye are a worthwhile tool within the framework of ventilation and filtration studies. The detection in real time of a specific particulate tracer allows characterization of ventilation behaviour such as air change rate, the determination of a good or bad mixing zone and transfer coefficient, or the determination of the decontamination factor for High Efficiency Particulate Air (HEPA) filters. Generally, these tests require specific aerosols in order to get rid of the atmospheric aerosol background. Until now the principle of fluorescent aerosol concentration measuring has only allowed an integral response with a time lag by means of sampling on filters and a fluorimetric analysis after specific conditioning of these filters. 5 refs., 13 figs.

  19. Fluorescence-based video profile beam diagnostics: Theory and experience

    SciTech Connect (OSTI)

    Sandoval, D.; Gilpatrick, D.; Shinas, M.; Garcia, R.; Yuan, V.; Zander, M.

    1994-05-01

    Inelastic collisions between accelerated particles and residual gas in the accelerator vessel can cause the residual gas to fluoresce. The gas fluorescence intensity is proportional to the current density of the particle beam. This process provides the foundation for a video diagnostic system to measure the profile and position of accelerated particle beams. This, in fact, has proven to be a useful diagnostic at several installations. This paper describes the light production process resulting from beam -- residual gas interactions and gives formulas for estimating the beam radiance for various conditions. Ground Test Accelerator (GTA) radiance calculations will be used as an example. In addition, measurement experiences with the GTA video diagnostics system will be discussed.

  20. Fluorescence-based video profile beam diagnostics: Theory and experience

    SciTech Connect (OSTI)

    Sandoval, D.P.; Garcia, R.C.; Gilpatrick, J.D.; Shinas, M.A.; Wright, R.; Yuan, V.; Zander, M.E. )

    1994-10-10

    Inelastic collisions between accelerated particles and residual gas in the accelerator vessel can cause the residual gas to fluoresce. The gas fluorescence intensity is proportional to the current density of the particle beam. This process provides the foundation for a video diagnostics system to measure the profile and position of accelerated particle beams. This, in fact, has proven to be a useful diagnostic at several installations. This paper describes the light production process resulting from beam-residual gas interactions and gives formulas for estimating the beam radiance for various conditions. Ground Test Accelerator (GTA) radiance calculations will be used as an example. In addition, measurement experiences with the GTA video diagnostics system will be discussed.

  1. DNA fragment sizing and sorting by laser-induced fluorescence

    DOE Patents [OSTI]

    Hammond, Mark L.; Jett, James H.; Keller, Richard A.; Marrone, Babetta L.; Martin, John C.

    1996-01-01

    A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.

  2. Rotational order–disorder structure of fluorescent protein FP480

    SciTech Connect (OSTI)

    Pletnev, Sergei; Morozova, Kateryna S.; Verkhusha, Vladislav V.; Dauter, Zbigniew

    2009-09-01

    An analysis of the rotational order–disorder structure of fluorescent protein FP480 is presented. In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order–disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

  3. Magnetic fluorescent lamp having reduced ultraviolet self-absorption

    DOE Patents [OSTI]

    Berman, Samuel M.; Richardson, Robert W.

    1985-01-01

    The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly (10) is enhanced by providing means (30) for establishing a magnetic field with lines of force along the path of electron flow through the bulb (12) of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

  4. Operating temperatures of recessed fluorescent fixtures with thermal insulation

    SciTech Connect (OSTI)

    Yarbrough, D.W.; Toor, I.A.

    1981-05-01

    Tests were performed to determine steady state surface temperatures for recessed fluorescent fixtures operated with and without thermal insulation on the top side of the fixture and to identify potential problems associated with the installation of thermal insulation. In addition to measuring temperatures, means were sought by which the fixtures can be thermally insulated and operated without fire hazards or damage to the fixture. (MCW)

  5. Contraband Detection with Nuclear Resonance Fluorescence: Feasibility and Impact

    SciTech Connect (OSTI)

    Pruet, J; Lange, D

    2007-01-03

    In this report they show that cargo interrogation systems developed to thwart trafficking of illicit nuclear materials could also be powerful tools in the larger fight against contraband smuggling. In particular, in addition to detecting special nuclear materials, cargo scanning systems that exploit nuclear resonance fluorescence to detect specific isotopes can be used to help find: chemical weapons; some drugs as well as some chemicals regulated under the controlled substances act; precious metals; materials regulated under export control laws; and commonly trafficked fluorocarbons.

  6. Enhanced detection of fluorescence quenching in labeled cells

    DOE Patents [OSTI]

    Crissman, Harry A.; Steinkamp, John A.

    1992-01-01

    A method is provided for quantifying BrdU labeled DNA in cells. The BrdU is incorporated into the DNA and the DNA is stained with a first fluorochrome having a fluorescence which is quenchable by BrdU. The first fluorochrome is preferably a thymidine base halogen analogue, such as a Hoechst fluorochrome. The DNA is then stained with a second fluorochrome having a fluorescence that is substantially uneffected by BrdU. The second fluorochrome may be selected from the group consisting of mithramycin, chromomycin A3, olivomycin, propidium iodide and ethidium bromine. The fluorescence from the first and second fluorochromes is then measured to obtain first and second output signals, respectively. The first output signal is substracted from the second output signal to obtain a difference signal which is functionally related to the quantity of BrdU incorporated into DNA. The technique is particularly useful for quantifying the synthesis of DNA during the S-phase of the cell cycle.

  7. Enhanced detection of fluorescence quenching in labeled cells

    DOE Patents [OSTI]

    Crissman, H.A.; Steinkamp, J.A.

    1987-11-30

    A method is provided for quantifying BrdU labeled DNA in cells. The BrdU is substituted onto the DNA and the DNA is stained with a first fluorochrome having a fluorescence which is quenchable by BrdU. The first fluorochrome is preferably a thymidine base halogen analogue, such as a Hoechst fluorochrome. The DNA is then stained with a second fluorochrome having a fluorescence which is substantially uneffected by BrdU. The second fluorochrome may be selected from the group consisting of mithramycin, chromomycin A3, olivomycin, propidium iodide and ethidium bromine. The fluorescence from the first and second fluorochromes is then measured to obtain first and second output signals, respectively. The first output signal is subtracted from the second output signal to obtain a difference signal which is functionally related to the quantity of BrdU incorporated into DNA. The technique is particularly useful for quantifying the synthesis of DNA during the S-phase of the cell cycle. 2 figs.

  8. The use of fluorescent compounds and complexes of metals as early warning detectors for corrosion

    SciTech Connect (OSTI)

    Johnson, R.E.; Agarwala, V.S.

    1994-12-31

    Several compounds have been identified which change their fluorescent behavior upon oxidation or reduction. These compounds have been investigated to determine the effects of temperature, pH, reduction conditions and re-oxidation behavior on their fluorescence. They have been incorporated into primer paint coatings for aluminum alloys and were found to fluoresce if the coating was scratched and exposed to air and moisture. Another compound was found to fluoresce with aluminum ions and its fluorescence behavior with varying conditions was also investigated. This compound, if added to a primer coating on an aluminum alloy, should fluoresce when aluminum ions begin to form as the alloy corrodes. Both of these methods appear to have potential to be early warning sensors for corrosion. Large surface areas of alloy material, such as are used in aircraft, could be scanned with UV light to detect the fluorescence of these materials indicating possible corrosion sites.

  9. Proton-induced x-ray fluorescence CT imaging

    SciTech Connect (OSTI)

    Bazalova-Carter, Magdalena Xing, Lei; Ahmad, Moiz; Matsuura, Taeko; Takao, Seishin; Shirato, Hiroki; Umegaki, Kikuo; Matsuo, Yuto; Fahrig, Rebecca

    2015-02-15

    Purpose: To demonstrate the feasibility of proton-induced x-ray fluorescence CT (pXFCT) imaging of gold in a small animal sized object by means of experiments and Monte Carlo (MC) simulations. Methods: First, proton-induced gold x-ray fluorescence (pXRF) was measured as a function of gold concentration. Vials of 2.2 cm in diameter filled with 0%–5% Au solutions were irradiated with a 220 MeV proton beam and x-ray fluorescence induced by the interaction of protons, and Au was detected with a 3 × 3 mm{sup 2} CdTe detector placed at 90° with respect to the incident proton beam at a distance of 45 cm from the vials. Second, a 7-cm diameter water phantom containing three 2.2-diameter vials with 3%–5% Au solutions was imaged with a 7-mm FWHM 220 MeV proton beam in a first generation CT scanning geometry. X-rays scattered perpendicular to the incident proton beam were acquired with the CdTe detector placed at 45 cm from the phantom positioned on a translation/rotation stage. Twenty one translational steps spaced by 3 mm at each of 36 projection angles spaced by 10° were acquired, and pXFCT images of the phantom were reconstructed with filtered back projection. A simplified geometry of the experimental data acquisition setup was modeled with the MC TOPAS code, and simulation results were compared to the experimental data. Results: A linear relationship between gold pXRF and gold concentration was observed in both experimental and MC simulation data (R{sup 2} > 0.99). All Au vials were apparent in the experimental and simulated pXFCT images. Specifically, the 3% Au vial was detectable in the experimental [contrast-to-noise ratio (CNR) = 5.8] and simulated (CNR = 11.5) pXFCT image. Due to fluorescence x-ray attenuation in the higher concentration vials, the 4% and 5% Au contrast were underestimated by 10% and 15%, respectively, in both the experimental and simulated pXFCT images. Conclusions: Proton-induced x-ray fluorescence CT imaging of 3%–5% gold solutions in a

  10. Parents and Kids: Energize Your Summer | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Did you know: Incandescent light bulbs only convert about 10 percent of the energy they consume into light and the rest is released as heat. The Energy Department's Energy Bike demonstrates the physical effort it takes to power incandescent, compact fluorescent and LED light bulbs. Students from Churchill Road Elementary School in Virginia recently pedaled for power at their Earth Day assembly, learning firsthand about energy efficiency. | Photo courtesy of the Energy Department. Did you know:

  11. Office Energy Checklist | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Office Energy Checklist Office Energy Checklist This checklist outlines actions that conserve energy at the office. Checkbox Replace incandescent lights with compact fluorescent lights (CFLs) or light-emitting diodes (LEDs) for desk lamps and overhead lighting. Using CFLs instead of comparable incandescent bulbs can save about 50% on your lighting costs. CFLs use only one-fourth the energy and last up to 10 times longer. Checkbox Switch off all unnecessary lights. Use dimmers, motion sensors, or

  12. ISSUANCE 2014-12-29: Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

    Office of Energy Efficiency and Renewable Energy (EERE)

    Energy Conservation Program: Clarification for Energy Conservation Standards and Test Procedures for Fluorescent Lamp Ballasts

  13. ISSUANCE 2016-03-24: Energy Conservation Program: Clarification of Test Procedures for Fluorescent Lamps Ballasts, Final Rule

    Office of Energy Efficiency and Renewable Energy (EERE)

    Energy Conservation Program: Clarification of Test Procedures for Fluorescent Lamps Ballasts, Final Rule

  14. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

    SciTech Connect (OSTI)

    Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka

    2015-07-31

    Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS{sup SV40}) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesis takes place. GFP-NLS{sup SV40} in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS{sup SV40} formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS{sup SV40} likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS{sup SV40} can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus.

  15. Occupational exposure in the fluorescent lamp recycling sector in France

    SciTech Connect (OSTI)

    Zimmermann, François Lecler, Marie-Thérèse; Clerc, Frédéric; Chollot, Alain; Silvente, Eric; Grosjean, Jérome

    2014-07-15

    Highlights: • Chemical risks were assessed in the five fluorescent lamp recycling facilities. • The main hazardous agents are mercury vapors and dust containing lead and yttrium. • Exposure and pollutant levels were correlated with steps and processes. • All the stages and processes are concerned by worrying levels of pollutants. • We suggest recommendations to reduce chemical risk. - Abstract: The fluorescent lamp recycling sector is growing considerably in Europe due to increasingly strict regulations aimed at inciting the consumption of low energy light bulbs and their end-of-life management. Chemical risks were assessed in fluorescent lamp recycling facilities by field measurement surveys in France, highlighting that occupational exposure and pollutant levels in the working environment were correlated with the main recycling steps and processes. The mean levels of worker exposure are 4.4 mg/m{sup 3}, 15.4 μg/m{sup 3}, 14.0 μg/m{sup 3}, 247.6 μg/m{sup 3}, respectively, for total inhalable dust, mercury, lead and yttrium. The mean levels of airborne pollutants are 3.1 mg/m{sup 3}, 9.0 μg/m{sup 3}, 9.0 μg/m{sup 3}, 219.2 μg/m{sup 3}, respectively, for total inhalable dust, mercury, lead and yttrium. The ranges are very wide. Surface samples from employees’ skin and granulometric analysis were also carried out. The overview shows that all the stages and processes involved in lamp recycling are concerned by the risk of hazardous substances penetrating into the bodies of employees, although exposure of the latter varies depending on the processes and tasks they perform. The conclusion of this study strongly recommends the development of a new generation of processes in parallel with more information sharing and regulatory measures.

  16. Blue-green phosphor for fluorescent lighting applications

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  17. Evaluating the variability of ceramics with x-ray fluorescence

    SciTech Connect (OSTI)

    Crown, P.L.; Schwalbe, L.A.; London, J.R.

    1984-01-01

    Our assessment of prehistoric trade in ceramics depends on our ability to identify and distinguish different sources of manufacture. For the American Southwest, archaeologists have proposed various models of ceramic manufacture and exchange. Until recently, conflicting hypotheses were tested mainly on the basis of petrographic analysis of nonplastic tempering materials. We have extended these analyses to include x-ray fluorescence (XRF). XRF provides a fast and inexpensive means of analyzing large numbers of samples. Since 1982, approximately 500 prehistoric sherds and 40 prepared clay and mineral samples have been examined with XRF. Multivariate statistical techniques have been applied to help identify groupings of samples with possible archaeological significance.

  18. A light diet for a giant appetite: An assessment of China's proposed fluorescent lamp standard

    SciTech Connect (OSTI)

    Lin, Jiang

    2002-04-11

    Lighting has been one of the fastest growing electric end-uses in China over the last twenty years, with an average annual growth rate of 14%. Fluorescent lighting provides a significant portion of China's lighting need. In 1998, China produced 680 million fluorescent lamps, of which 420 million were linear fluorescent lamps of various diameters (T8 to T12). There are substantial variations both in energy efficiency and lighting performance among locally produced fluorescent lamps. Such variations present a perfect opportunity for policy intervention through efficiency standards to promote the adoption of more efficient fluorescent lamps in China. This paper analyzes China's proposed minimum efficiency standard for fluorescent lamps and presents an assessment of its likely impacts on China's lighting energy consumption and GHG emissions.

  19. Calculation of K-shell fluorescence yields for low-Z elements

    SciTech Connect (OSTI)

    Nekkab, M.; Kahoul, A.; Deghfel, B.; Aylikci, N. Küp; Aylikçi, V.

    2015-03-30

    The analytical methods based on X-ray fluorescence are advantageous for practical applications in a variety of fields including atomic physics, X-ray fluorescence surface chemical analysis and medical research and so the accurate fluorescence yields (ω{sub K}) are required for these applications. In this contribution we report a new parameters for calculation of K-shell fluorescence yields (ω{sub K}) of elements in the range of 11≤Z≤30. The experimental data are interpolated by using the famous analytical function (ω{sub k}/(1−ω{sub k})){sup 1/q} (were q=3, 3.5 and 4) vs Z to deduce the empirical K-shell fluorescence yields. A comparison is made between the results of the procedures followed here and those theoretical and other semi-empirical fluorescence yield values. Reasonable agreement was typically obtained between our result and other works.

  20. 2014-10-14 Issuance: Test Procedures Correction for Fluorescent Lamp

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ballasts; Notice of Proposed Rulemaking | Department of Energy 4 Issuance: Test Procedures Correction for Fluorescent Lamp Ballasts; Notice of Proposed Rulemaking 2014-10-14 Issuance: Test Procedures Correction for Fluorescent Lamp Ballasts; Notice of Proposed Rulemaking This document is a pre-publication Federal Register notice of proposed rulemaking regarding test procedures for fluorescent lamp ballasts, as issued by the Deputy Assistant Secretary for Energy Efficiency on October 14,

  1. Cerenkov emission induced by external beam radiation stimulates molecular fluorescence

    SciTech Connect (OSTI)

    Axelsson, Johan; Davis, Scott C.; Gladstone, David J.; Pogue, Brian W.

    2011-07-15

    Purpose: Cerenkov emission is induced when a charged particle moves faster than the speed of light in a given medium. Both x-ray photons and electrons produce optical Cerenkov photons in everyday radiation therapy of tissue; yet, this phenomenon has never been fully documented. This study quantifies the emissions and also demonstrates that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms. Methods: In this study, Cerenkov emission induced by radiation from a clinical linear accelerator is investigated. Biological mimicking phantoms were irradiated with x-ray photons, with energies of 6 or 18 MV, or electrons at energies 6, 9, 12, 15, or 18 MeV. The Cerenkov emission and the induced molecular fluorescence were detected by a camera or a spectrometer equipped with a fiber optic cable. Results: It is shown that both x-ray photons and electrons, at MeV energies, produce optical Cerenkov photons in tissue mimicking media. Furthermore, we demonstrate that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms. Conclusions: The results here indicate that molecular fluorescence monitoring during external beam radiotherapy is possible.

  2. Red phosphors for use in high CRI fluorescent lamps

    DOE Patents [OSTI]

    Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

    2005-11-15

    Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

  3. Protein- protein interaction detection system using fluorescent protein microdomains

    DOE Patents [OSTI]

    Waldo, Geoffrey S.; Cabantous, Stephanie

    2010-02-23

    The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

  4. INITIAL OPERATION OF THE LEDA BEAM-INDUCED FLUORESCENCE DIAGNOSTIC

    SciTech Connect (OSTI)

    J. KAMPERSCHROER; ET AL

    2000-06-01

    A diagnostic based on beam-induced fluorescence has been developed and used to examine the expanded beam in the High-Energy Beam Transport (HEBT) section of the Low Energy Demonstration Accelerator (LEDA). The system consists of a camera, a gas injector, a spectrometer, and a control system. Gas is injected to provide a medium for the beam to excite, the camera captures the resulting image of the fluorescing gas, and the spectrometer measures the spectrum of the emitted light. EPICS was used to control the camera and acquire and store images. Data analysis is presently being performed offline. A Kodak DCS420m professional CCD camera is the primary component of the optical system. InterScience, Inc. modified the camera with the addition of a gain of 4000 image intensifier, thereby producing an intensified camera with a sensitivity of {approximately}0.5 milli-lux. Light is gathered with a 1 inch format, 16-160 mm, Computar zoom lens. This lens is attached to the camera via a Century Precision Optics relay lens. Images obtained using only hydrogen from the beam stop exhibited features not yet understood. Images with good signal-to-noise ratio were obtained with the injection of sufficient nitrogen to raise the HEBT pressure to 2-8x10{sup {minus}6} torr. Two strong nitrogen lines, believed to be of the first negative group of N{sub 2}{sup +}, were identified at 391 and 428 nm.

  5. Analysis of the influence of subcellular localization of the HIV Rev protein on Rev-dependent gene expression by multi-fluorescence live-cell imaging

    SciTech Connect (OSTI)

    Wolff, Horst; Hadian, Kamyar; Ziegler, Manja; Weierich, Claudia; Kramer-Hammerle, Susanne; Kleinschmidt, Andrea; Erfle, Volker; Brack-Werner, Ruth . E-mail: brack@gsf.de

    2006-02-15

    The human immunodeficiency virus Rev protein is a post-transcriptional activator of HIV gene expression. Rev is a nucleocytoplasmic shuttle protein that displays characteristic nuclear/nucleolar subcellular localization in various cell lines. Cytoplasmic localization of Rev occurs under various conditions disrupting Rev function. The goal of this study was to investigate the relationship between localization of Rev and its functional activity in living cells. A triple-fluorescent imaging assay, called AQ-FIND, was established for automatic quantitative evaluation of nucleocytoplasmic distribution of fluorescently tagged proteins. This assay was used to screen 500 rev genes generated by error-prone PCR for Rev mutants with different localization phenotypes. Activities of the Rev mutants were determined with a second quantitative, dual-fluorescent reporter assay. In HeLa cells, the majority of nuclear Rev mutants had activities similar to wild-type Rev. The activities of Rev mutants with abnormal cytoplasmic localization ranged from moderately impaired to nonfunctional. There was no linear correlation between subcellular distribution and levels of Rev activity. In astrocytes, nuclear Rev mutants showed similar impaired activities as the cytoplasmic wild-type Rev. Our data suggest that steady-state subcellular localization is not a primary regulator of Rev activity but may change as a secondary consequence of altered Rev function. The methodologies described here have potential for studying the significance of subcellular localization for functions of other regulatory factors.

  6. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, R.J.; Small, G.J.; Shields, P.A.

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.

  7. Capillary electrophoresis-fluorescence line narrowing system (CE-FLNS) for on-line structural characterization

    DOE Patents [OSTI]

    Jankowiak, Ryszard J.; Small, Gerald J.; Shields, Peter A.

    1999-04-27

    Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes.

  8. Nuclear Resonance Fluorescence at MIT | U.S. DOE Office of Science...

    Office of Science (SC) Website

    Resonance Fluorescence at MIT Nuclear Physics (NP) NP Home About Research Facilities Science Highlights Benefits of NP Applications of Nuclear Science Applications of Nuclear Science ...

  9. Max Tech and Beyond: Fluorescent Lamps (Technical Report) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    In addition to offering the market a high-quality efficacious light source, another strong value proposition of fluorescent lighting is its long operating life. In today's market, ...

  10. Excitation-emission spectra and fluorescence quantum yields for fresh and aged biogenic secondary organic aerosols

    SciTech Connect (OSTI)

    Lee, Hyun Ji; Laskin, Alexander; Laskin, Julia; Nizkorodov, Sergey A.

    2013-05-10

    Certain biogenic secondary organic aerosols (SOA) become absorbent and fluorescent when exposed to reduced nitrogen compounds such as ammonia, amines and their salts. Fluorescent SOA may potentially be mistaken for biological particles by detection methods relying on fluorescence. This work quantifies the spectral distribution and effective quantum yields of fluorescence of SOA generated from two monoterpenes, limonene and a-pinene, and two different oxidants, ozone (O3) and hydroxyl radical (OH). The SOA was generated in a smog chamber, collected on substrates, and aged by exposure to ~100 ppb ammonia vapor in air saturated with water vapor. Absorption and excitation-emission matrix (EEM) spectra of aqueous extracts of aged and control SOA samples were measured, and the effective absorption coefficients and fluorescence quantum yields (~0.005 for 349 nm excitation) were determined from the data. The strongest fluorescence for the limonene-derived SOA was observed for excitation = 420+- 50 nm and emission = 475 +- 38 nm. The window of the strongest fluorescence shifted to excitation = 320 +- 25 nm and emission = 425 +- 38 nm for the a-pinene-derived SOA. Both regions overlap with the excitation-emission matrix (EEM) spectra of some of the fluorophores found in primary biological aerosols. Our study suggests that, despite the low quantum yield, the aged SOA particles should have sufficient fluorescence intensities to interfere with the fluorescence detection of common bioaerosols.