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  1. MHK Technologies/TUVALU | Open Energy Information

    Open Energy Info (EERE)

    to the MHK database homepage TUVALU.jpg Technology Profile Primary Organization Arlas Invest Technology Resource Click here Wave Technology Description The chain or cable coils in...

  2. Tuvalu: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Country Profile Name Tuvalu Population 10,837 GDP Unavailable Energy Consumption Quadrillion Btu 2-letter ISO code TV 3-letter ISO code TUV Numeric ISO code...

  3. Immosolar Spain | Open Energy Information

    Open Energy Info (EERE)

    Spain Jump to: navigation, search Logo: Immosolar Spain Name: Immosolar Spain Address: C Sigfrido 1, pl.2 of.21 Place: Barcelona, Spain Sector: Solar Product: Solar energy systems...

  4. TME Spain | Open Energy Information

    Open Energy Info (EERE)

    TME Spain Jump to: navigation, search Name: TME Spain Place: Spain Sector: Wind energy Product: Spanish firm TME, a privately owned company, currently holds equity investments in...

  5. SP Newsprint

    SciTech Connect (OSTI)

    2009-11-01

    This is a combined heat and power (CHP) project profile on a 45 MW steam turbine at SP Newsprint in Dublin, Georgia.

  6. Barcelona, Spain: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    is city in Spain. US Recovery Act Smart Grid Projects in Barcelona, Spain DERIREC 22@Microgrid (Smart Grid Project) Registered Energy Companies in Barcelona, Spain Agrupacio...

  7. Association of Renewable Energy Producers Spain | Open Energy...

    Open Energy Info (EERE)

    Renewable Energy Producers Spain Jump to: navigation, search Name: Association of Renewable Energy Producers (Spain) Place: Barcelona, Spain Zip: 8008 Sector: Renewable Energy...

  8. Renewable Energies and Photovoltaics Spain S L REPS | Open Energy...

    Open Energy Info (EERE)

    and Photovoltaics Spain S L REPS Jump to: navigation, search Name: Renewable Energies and Photovoltaics Spain S.L. (REPS) Place: Spain Sector: Solar Product: Spanish solar project...

  9. ARM - Instrument - sp2

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    govInstrumentssp2 Documentation ARM Data Discovery Browse Data Comments? We would love to hear from you! Send us a note below or call us at 1-888-ARM-DATA. Send Instrument : Single Particle Soot Photometer (SP2) Instrument Categories Aerosols The SP2 measures the soot (black carbon) mass of individual aerosol particles by laser-induced incandescence down to concentrations as low as 10 ng/m^3. The SP2 is part of the Aerosol Observing System (AOS). Output Datastreams sp2 : Single Particle Soot

  10. Extensible Argonne SP Scheduling System

    Energy Science and Technology Software Center (OSTI)

    1996-01-24

    The Argonne SP Scheduler provides deterministic queuing of jobs for exclusive access to the SP nodes for either batch or interactive use.

  11. Spain: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Spain: Energy Resources Jump to: navigation, search Loading map... "minzoom":false,"mappingservice":"googlemaps3","type":"TERRAIN","zoom":5,"types":"ROADMAP","SATELLITE","HYBRID"...

  12. RP Global Spain S A Minicentrales | Open Energy Information

    Open Energy Info (EERE)

    RP Global Spain S A Minicentrales Jump to: navigation, search Name: RP Global Spain S.A. (Minicentrales) Place: Madrid, Spain Zip: E - 28036 Product: The Spanish arm of RP Global,...

  13. EWIS European wind integration study (Smart Grid Project) (Spain...

    Open Energy Info (EERE)

    Spain) Jump to: navigation, search Project Name EWIS European wind integration study Country Spain Coordinates 40.522152, -4.163818 Loading map... "minzoom":false,"mappingserv...

  14. Earth plus Mars: Los Alamos National Lab partners with Spain...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Earth plus Mars: Los Alamos National Lab partners with Spain and France Earth plus Mars: Los Alamos National Lab partners with Spain and France New Mexico's role in the next...

  15. BeyWatch (Smart Grid Project) (Spain) | Open Energy Information

    Open Energy Info (EERE)

    BeyWatch (Smart Grid Project) (Spain) Jump to: navigation, search Project Name BeyWatch Country Spain Coordinates 40.65564, -4.075928 Loading map... "minzoom":false,"mappingse...

  16. Cameron, LA Liquefied Natural Gas Exports to Spain (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Spain (Million Cubic Feet) Cameron, LA Liquefied Natural Gas Exports to Spain (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2011 2,911 - No Data ...

  17. Sabine Pass, LA Exports to Spain Liquefied Natural Gas (Million...

    U.S. Energy Information Administration (EIA) Indexed Site

    Spain Liquefied Natural Gas (Million Cubic Feet) Sabine Pass, LA Exports to Spain Liquefied Natural Gas (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec ...

  18. Enel Green Power SpA formerly Erga SpA | Open Energy Information

    Open Energy Info (EERE)

    SpA formerly Erga SpA Jump to: navigation, search Name: Enel Green Power SpA (formerly Erga SpA) Place: Pisa, Italy Zip: 56122 Sector: Geothermal energy, Hydro, Renewable Energy,...

  19. Solarcell SpA | Open Energy Information

    Open Energy Info (EERE)

    SpA Jump to: navigation, search Name: Solarcell SpA Place: Brugherio, Italy Product: Italian manufacturer of PV cells. References: Solarcell SpA1 This article is a stub. You...

  20. Cyanobacterium sp. for production of compounds

    DOE Patents [OSTI]

    Piven, Irina; Friedrich, Alexandra; Duhring, Ulf; Uliczka, Frank; Baier, Kerstin; Inaba, Masami; Shi, Tuo; Wang, Kui; Enke, Heike; Kramer, Dan

    2015-10-13

    A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.

  1. Innova SpA | Open Energy Information

    Open Energy Info (EERE)

    to: navigation, search Name: Innova SpA Place: Italy Sector: Services Product: General Financial & Legal Services ( Private family-controlled ) References: Innova SpA1 This...

  2. ARM - Campaign Instrument - sp2

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    would love to hear from you Send us a note below or call us at 1-888-ARM-DATA. Send Campaign Instrument : Single Particle Soot Photometer (SP2) Instrument Categories Aerosols...

  3. Major tanker spill off Spain under control

    SciTech Connect (OSTI)

    Not Available

    1992-12-14

    This paper reports that a 23 sq mile oil slick along Spain's northwest coast, spreading form the wreckage of the Greek oil tanker Aegean Sea, was for the most part under control as of Dec. 10, Spanish authorities reported. Various press reports put the total spill volume at 490,000 bbl, about double that leaked by the Exxon Valdez supertanker off Alaska in 1989. If initial reports of the spill volume are borne out, the Aegean Sea spill would rank at least as one of the 10 biggest tanker spills.

  4. Argonne's SpEC Module

    SciTech Connect (OSTI)

    Harper, Jason

    2014-05-05

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  5. Argonne's SpEC Module

    ScienceCinema (OSTI)

    Harper, Jason

    2014-06-05

    Jason Harper, an electrical engineer in Argonne National Laboratory's EV-Smart Grid Interoperability Center, discusses his SpEC Module invention that will enable fast charging of electric vehicles in under 15 minutes. The module has been licensed to BTCPower.

  6. Fortesa SpA | Open Energy Information

    Open Energy Info (EERE)

    Fortesa SpA Place: Italy Sector: Solar Product: Italy-based developer of PV solar plants. References: Fortesa SpA1 This article is a stub. You can help OpenEI by expanding...

  7. Metalco SpA | Open Energy Information

    Open Energy Info (EERE)

    Metalco SpA Jump to: navigation, search Name: Metalco SpA Place: Italy Product: Italy-based active metal construction company offering solutions for PV applications. References:...

  8. Almeco SpA | Open Energy Information

    Open Energy Info (EERE)

    Almeco SpA Jump to: navigation, search Name: Almeco SpA Place: Milan, Italy Zip: 20098 Product: Milan-based lighting company. Manufactures daylight saving systems and light pipes...

  9. Enia SpA | Open Energy Information

    Open Energy Info (EERE)

    SpA Jump to: navigation, search Name: Enia SpA Place: Parma, Italy Zip: 43100 Product: Italian utility company. Coordinates: 44.79864, 10.339925 Show Map Loading map......

  10. Argonne's SpEC Module | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SpEC Module Share Topic Energy Energy efficiency Vehicles

  11. Enel SpA | Open Energy Information

    Open Energy Info (EERE)

    Enel SpA Place: Roma, Italy Zip: 198 Sector: Renewable Energy Product: Rome-based electricity distributor and generator, also supplies gas and is active in telecommunications....

  12. SP Energy Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    Energy Co Ltd Jump to: navigation, search Name: SP Energy Co Ltd Place: Moongyeong, North Gyeongsang, Korea (Republic) Product: Korea-based project developer involved with building...

  13. ,"Liquefied U.S. Natural Gas Re-Exports to Spain (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Natural Gas Re-Exports to Spain (Million Cubic Feet)" "Sourcekey","NGMEPG0ERENUS-NSPMMCF" "Date","Liquefied U.S. Natural Gas Re-Exports to Spain (Million Cubic Feet)" ...

  14. SunTergrid SpA | Open Energy Information

    Open Energy Info (EERE)

    SunTergrid SpA Jump to: navigation, search Name: SunTergrid SpA Place: Roma, Italy Sector: Solar Product: Italy-based solar project developer and subsidiary of Terna SpA....

  15. Solar Utility SpA | Open Energy Information

    Open Energy Info (EERE)

    Utility SpA Jump to: navigation, search Name: Solar Utility SpA Place: Italy Sector: Solar Product: Italy-based solar PV project developer. References: Solar Utility SpA1 This...

  16. Compagnia Italiana Energia SpA CIE | Open Energy Information

    Open Energy Info (EERE)

    Compagnia Italiana Energia SpA CIE Jump to: navigation, search Name: Compagnia Italiana Energia SpA (CIE) Place: Torino, Italy Zip: 10143 Sector: Renewable Energy Product:...

  17. SP-100, the US Space Nuclear Reactor Power Program. Technical...

    Office of Scientific and Technical Information (OSTI)

    Technical Report: SP-100, the US Space Nuclear Reactor Power Program. Technical information report Citation Details In-Document Search Title: SP-100, the US Space Nuclear Reactor ...

  18. Enel Produzione SpA | Open Energy Information

    Open Energy Info (EERE)

    Produzione SpA Jump to: navigation, search Name: Enel Produzione SpA Place: Roma, Italy Zip: 198 Sector: Biomass, Geothermal energy, Hydro, Services, Wind energy Product:...

  19. EASY-DOS. Extensible Argonne SP Scheduling sYstem

    SciTech Connect (OSTI)

    Lifka, D.; Henderson, M.; Rayl, K.

    1995-05-01

    The Argonne SP Scheduler provides deterministic queuing of jobs for exclusive access to the SP nodes for either batch or interactive use.

  20. EASY-MAC. Extensible Argonne SP Scheduling sYstem

    SciTech Connect (OSTI)

    Lifka, D.; Henderson, M.; Rayl, K.

    1995-05-01

    The Argonne SP Scheduler provides deterministic queuing of jobs for exclusive access to the SP nodes for either batch or interactive use.

  1. Launch vehicle integration requirements for SP-100

    SciTech Connect (OSTI)

    Shaw, L.T. Jr.; Womack, J.R.

    1984-01-31

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 gives rise to a set of issues that require special attention to assure that payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compability between SP-100 systems and the NSTS.

  2. Edison SpA | Open Energy Information

    Open Energy Info (EERE)

    SpA Place: Milan, Italy Zip: 20121 Sector: Hydro, Wind energy Product: Independent power producer with approximately 9,300MW of hydro, fossil fuel and wind generation assets....

  3. ARM - Campaign Instrument - sp2-air

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    -air Comments? We would love to hear from you Send us a note below or call us at 1-888-ARM-DATA. Send Campaign Instrument : Single Particle Soot Photometer - Airborne (SP2-AIR)...

  4. Pramac SpA | Open Energy Information

    Open Energy Info (EERE)

    Italy Zip: 53031 Product: Pramac is predominately a manufacturer of handling systems and power systems, entered the PV industry in December 2007. References: Pramac SpA1 This...

  5. HERA SpA | Open Energy Information

    Open Energy Info (EERE)

    HERA SpA Place: Bologna, Italy Zip: 40127 Sector: Services Product: Hera is a large Italian multiple-service group, born as the result of the first significant aggregation of...

  6. Ecological compensation and Environmental Impact Assessment in Spain

    SciTech Connect (OSTI)

    Villarroya, Ana; Puig, Jordi

    2010-11-15

    To achieve meaningful sustainable development, Environmental Impact Assessment (EIA) should avoid the net losses in the environment resource base. But EIA practice does not always avoid the losses caused by the implementation of the projects under EIA regulation. Some environmental impacts are, simply, admitted, even without enforcing any form of compensation. When applied, compensation is sometimes just a monetary payment to offset the environmental loss. This paper looks for evidence on the role that compensation is given at present in EIA practice in Spain, and for some of its conceptual and regulatory roots. Specifically, it explores how compensation is addressed in 1302 records of decision (RODs) on those projects subject to the Spanish EIA regulation published during the years 2006 and 2007, to know how far Spain is from preserving the environmental resource base managed through this particular aspect of EIA practice. As a result, it is concluded that the practice of ecological compensation in EIA in Spain is much lower than it could be expected in a theoretical sustainability context committed to avoid net losses in the environment resource base, mainly due to an EIA practice focused on on-site mitigation that allows these net losses.

  7. U.S. and Spain to Develop Solar Decathlon Europe | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Spain to Develop Solar Decathlon Europe U.S. and Spain to Develop Solar Decathlon Europe October 18, 2007 - 3:21pm Addthis WASHINGTON, DC - Today U.S. Department of Energy (DOE) Assistant Secretary for Energy Efficiency and Renewable Energy Alexander Karsner and Spain's Undersecretary of Housing Fernando Magro Fernández signed a Memorandum of Understanding (MOU) to collaborate in the development of a Solar Decathlon Europe competition in 2010. The Solar Decathlon is a competition launched by

  8. TerniEnergia SpA | Open Energy Information

    Open Energy Info (EERE)

    TerniEnergia SpA Jump to: navigation, search Name: TerniEnergia SpA Place: Terni, Italy Zip: 5100 Sector: Renewable Energy Product: Italy-based developer of renewable energy...

  9. Fintel Energy Group SpA | Open Energy Information

    Open Energy Info (EERE)

    Fintel Energy Group SpA Jump to: navigation, search Name: Fintel Energy Group SpA Place: Pollenza, Italy Zip: 62010 Sector: Renewable Energy Product: Italy-based energy operator...

  10. Microsoft Word - FY 2015 SP Performance Appraisal System Guidance - Final

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Office of Executive Resources Office of the Chief Human Capital Officer U.S. Department of Energy FY 2015 Senior Professional (SP) Performance Appraisal System Opening Guidance 2 Table of Contents Contents I. SP PERFORMANCE APPRAISAL TEMPLATE and TIMELINE ....................................................................... 3 II. PERFORMANCE PLAN - CRITICAL ELEMENTS .......................................................................................... 3 III. SP SUMMARY RATING LEVEL

  11. U.S. Liquefied Natural Gas Exports to Spain (Million Cubic Feet...

    U.S. Energy Information Administration (EIA) Indexed Site

    Exports to Spain (Million Cubic Feet) U.S. Liquefied Natural Gas Exports to Spain (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2010 0 3,149 0 0 0 0 0 0 ...

  12. ARM - Campaign Instrument - sp-ams

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    govInstrumentssp-ams Comments? We would love to hear from you! Send us a note below or call us at 1-888-ARM-DATA. Send Campaign Instrument : Soot particle aerosol mass spectrometer (SP-AMS) Instrument Categories Aerosols Primary Measurements Taken The following measurements are those considered scientifically relevant. Refer to the datastream (netcdf) file headers for the list of all available measurements, including those recorded for diagnostic or quality assurance purposes. Inorganic chemical

  13. FY 2015 SENIOR EXECUTIVE SERVICE (SES) AND SENIOR PROFESSIONAL (SP)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PERFORMANCE APPRAISAL CYCLE - CLOSEOUT GUIDANCE | Department of Energy 5 SENIOR EXECUTIVE SERVICE (SES) AND SENIOR PROFESSIONAL (SP) PERFORMANCE APPRAISAL CYCLE - CLOSEOUT GUIDANCE FY 2015 SENIOR EXECUTIVE SERVICE (SES) AND SENIOR PROFESSIONAL (SP) PERFORMANCE APPRAISAL CYCLE - CLOSEOUT GUIDANCE The FY 2015 performance appraisal cycle for both SES and SP members, which includes Senior Level (SL) and Scientific or Professional (ST) employees, concluded on September 30, 2015. In order to

  14. FY 2016 SENIOR EXECUTIVE SERVICE (SES) AND SENIOR PROFESSIONAL (SP)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PERFORMANCE APPRAISAL OPENING GUIDANCE | Department of Energy 6 SENIOR EXECUTIVE SERVICE (SES) AND SENIOR PROFESSIONAL (SP) PERFORMANCE APPRAISAL OPENING GUIDANCE FY 2016 SENIOR EXECUTIVE SERVICE (SES) AND SENIOR PROFESSIONAL (SP) PERFORMANCE APPRAISAL OPENING GUIDANCE FY 2016 performance appraisal cycle for both SES and SP members, which includes Senior Level (SL) and Scientific or Professional (ST) employees, concludes on December 15, 2015. These documents provide a detailed overview of

  15. Mineralization of a Malaysian crude oil by Pseudomonas sp. and Achromabacter sp. isolated from coastal waters

    SciTech Connect (OSTI)

    Ahmad, J.; Ahmad, M.F.

    1995-12-31

    Regarded as being a potentially effective tool to combat oil pollution, bioremediation involves mineralization, i.e., the conversion of complex hydrocarbons into harmless CO{sub 2} and water by action of microorganisms. Therefore, in achieving optimum effectiveness from the application of these products on crude oil in local environments, the capability of the bacteria to mineralize hydrocarbons was evaluated. The microbial laboratory testing of mineralization on local oil degraders involved, first, isolation of bacteria found at a port located on the west coast of Peninsular Malaysia. Subsequently, these bacteria were identified by means of Biomereux`s API 20E and 20 NE systems and later screened by their growth on a Malaysian crude oil. Selected strains of Pseudomonas sp. and Achromabacter sp. were then exposed individually to a similar crude oil in a mineralization unit and monitored for 16 days for release of CO{sub 2}. Pseudomonas paucimobilis was found to produce more CO{sub 2} than Achromobacter sp. When tested under similar conditions, mixed populations of these two taxa produced more CO{sub 2} than that produced by any individual strain. Effective bioremediation of local crude in Malaysian waters can therefore be achieved from biochemically developed Pseudomonas sp. strains.

  16. ,"Liquefied U.S. Natural Gas Re-Exports to Spain (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    2016 11:48:54 AM" "Back to Contents","Data 1: Liquefied U.S. Natural Gas Re-Exports to Spain (Million Cubic Feet)" "Sourcekey","NGMEPG0ERENUS-NSPMMCF" "Date","Liquefied U.S....

  17. Current status of the radioactive waste management programme in Spain

    SciTech Connect (OSTI)

    Lang-Lenton Leon, Jorge; Garcia Neri, Emilio

    2007-07-01

    Since 1984, ENRESA is responsible of the radioactive waste management and the decommissioning of nuclear installations in Spain. The major recent challenge has been the approval of the Sixth General Radioactive Waste Plan (GRWP) as 'master plan' of the activities to be performed by ENRESA. Regarding the LILW programme, the El Cabril LILW disposal facility will be described highlighting the most relevant events especially focused on optimizing the existing capacity and the start-up of a purpose -built disposal area for VLLW. Concerning the HLW programme, two aspects may be distinguished in the direct management of spent fuel: temporary storage and long-term management. In this regards, a major challenge has been the decision adopted by the Spanish Government to set up a Inter-ministerial Committee for the establishment of the criteria that must be met by the site of the Centralized Intermediate Storage (CTS) facility as the first and necessary step for the process. Also the developments of the long-term management programme will be presented in the frame of the ENRESA's R and D programme. Finally, in the field of decommissioning they will be presented the PIMIC project at the CIEMAT centre and the activities in course for the decommissioning of Jose Cabrera NPP. (authors)

  18. Preliminary SP-100/Stirling heat exchanger designs

    SciTech Connect (OSTI)

    Schmitz, P.; Tower, L.; Dawson, R.; Blue, B.; Dunn, P.

    1994-09-01

    Analytic modeling of several heat exchanger concepts to couple the SP-100 nuclear reactor lithium loop and the Space Stirling Power Convertor (SSPC) was performed. Four 25 kWe SSPC`s are used to produce the required 100 kW of electrical power. This design work focused on the interface between a single SSPC and the primary lithium loop. Manifolding to separate and collect the four channel flow was not modeled. This work modeled two separate types of heat exchanger interfaces (conductive coupling and radiative coupling) to explore their relative advantages and disadvantages. The minimum mass design of the conductively coupled concepts was 18 kg or 0.73 kg/kWe for a single 25 kWe convertor. The minimum mass radiatively coupled concept was 41 kg or 1.64 kg/kWe. The direct conduction heat exchanger provides a lighter weight system because of its ability to operate the Stirling convertor evaporator at higher heat fluxes than those attainable by the radiatively coupled systems. Additionally the conductively coupled concepts had relatively small volumes and provide potentially simpler assembly. Their disadvantages were the tight tolerances and material joining problems associated with this refractory to superalloy interface. The advantages of the radiatively coupled designs were the minimal material interface problems.

  19. PaSol Italia SpA | Open Energy Information

    Open Energy Info (EERE)

    Name: PaSol Italia SpA Place: Varallo Pombia, Italy Zip: 28040 Sector: Solar Product: PA.SOL was formed by local private investors in order to initiate local PV module...

  20. Launch vehicle integration requirements for SP-100. Technical information report

    SciTech Connect (OSTI)

    Shaw, L.T. Jr.; Womack, J.R.

    1984-03-01

    SP-100 is the designation for a nuclear reactor-based power plant being developed for both civil and military missions beginning in the 1990s for such potential space applications as communication satellites, space radar, electric propulsion and space stations. Typically, a system using the SP-100 along with a selected upper stage system would be launched by the National Space Transportation System (NSTS) Space Shuttle System into a near-earth orbit, deployed, and through upper stage propulsion burn(s) be inserted/transferred to its mission orbit. The nature of the advanced design SP-100 payloads using this power plant are physically and functionally compatible with the NSTS and meet the safety requirements thereof. The purpose of this document is to define and present the requirements and interface provisions that, when satisfied, will ensure technical compatibility between SP-100 systems and the NSTS.

  1. MX Group SpA | Open Energy Information

    Open Energy Info (EERE)

    SpA Place: Villasanta, Italy Zip: 20058 Product: MX group is a turnkey provider of manufacturing plants for PV and other electronic equipment. Coordinates: 45.606895, 9.3066...

  2. Shewanella loihica sp. nov., isolated from iron-rich microbial...

    Office of Scientific and Technical Information (OSTI)

    loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean A novel marine bacterial strain, PV-4T, isolated from a microbial mat located at a hydrothermal vent...

  3. Earth plus Mars: Los Alamos National Lab partners with Spain and France

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Week

    Earth plus Mars: Los Alamos National Lab partners with Spain and France Earth plus Mars: Los Alamos National Lab partners with Spain and France New Mexico's role in the next mission to Mars will also be part of a tri-national Earthly collaboration, officials say. June 26, 2015 New Mexico's role in the next mission to Mars will also be part of a tri-national Earthly collaboration, officials say. New Mexico's role in the next mission to Mars will also be part of a tri-national Earthly

  4. Three-dimensional carbon allotropes comprising phenyl rings and acetylenic chains in sp+sp2 hybrid networks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wang, Jian -Tao; Chen, Changfeng; Li, Han -Dong; Mizuseki, Hiroshi; Kawazoe, Yoshiyuki

    2016-04-18

    Here, we here identify by ab initio calculations a new type of three-dimensional (3D) carbon allotropes that consist of phenyl rings connected by linear acetylenic chains in sp+sp2 bonding networks. These structures are constructed by inserting acetylenic or diacetylenic bonds into an all sp2-hybridized rhombohedral polybenzene lattice, and the resulting 3D phenylacetylene and phenyldiacetylene nets comprise a 12-atom and 18-atom rhombohedral primitive unit cells R-3m symmetry, which are characterized as the 3D chiral crystalline modification of 2D graphyne and graphdiyne, respectively. Simulated phonon spectra reveal that these structures are dynamically stable. Electronic band calculations indicate that phenylacetylene is metallic, whilemore » phenyldiacetylene is a semiconductor with an indirect band gap of 0.58 eV. The present results establish a new type of carbon phases and offer insights into their outstanding structural and electronic properties.« less

  5. ErgyCapital SpA formerly Greenergy Capital | Open Energy Information

    Open Energy Info (EERE)

    SpA formerly Greenergy Capital Jump to: navigation, search Name: ErgyCapital SpA (formerly Greenergy Capital) Place: Milan, Italy Zip: 20121 Sector: Efficiency, Renewable Energy...

  6. NSS5/SP-STM2 Joint International Conference

    SciTech Connect (OSTI)

    Saw-Wai Hla

    2009-05-03

    The NSS5/SP-STM2 conference was held in Athens, Ohio July 15-19, 2008. The conference brought together a prestigious group of scientists from all over the globe to focus for 3 days on a variety of nanoscience topics, particularly on nanoscale spectroscopy and spin-polarized scanning tunneling microscopy. The conference was attended by many young scientists as well as senior scientists. Attendees to the conference were drawn from more than 10 countries and included 28 invited speakers, who are the leading scientists in their respective research areas. Included among the invited speakers were 4 plenary speakers - eminent scientists in their fields. The conference was divided into two parallel sessions the NSS5 session and the SP-STM2 session.

  7. The transputer based GA. SP data acquisition system

    SciTech Connect (OSTI)

    Colombo, D.; Avano, B.; DePoli, M.; Maron, G. ); Negro, A.; Parlati, G. )

    1992-04-01

    In this paper, the new data acquisition for the GA.SP detector is presented. It is a distributed system based on a network of 40 T800 and T222 transputers linked to a VME system used for histogram storage. A 100 MBit/s FDDI ring connects the system to UNIX workstations used for the experiment control, histogram display and second level data analysis.

  8. Multiplexer/amplifier test results for SP-100

    SciTech Connect (OSTI)

    King, D.B.; Luker, S.M. ); Ryan, R. )

    1990-01-01

    Multiplexer and amplifier systems must be designed with transistors that can perform satisfactorily over ten years to a total gamma dose of 120E6 rads and a total neutron fluence of 1.6E15 nvt for the SP-100 reactor system. Series of gamma and neutron tests have been completed to measure transistor degradation as a function of total dose, fluence, and temperature. Test results indicate that modest increases in temperature result in substantial improvement of transistor performance at a neutron flux of 8E8 n/cm{sup 2}/s. 2 refs., 3 figs.

  9. Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability presentation at the April 2013 peer review meeting held in Denver, Colorado. hawaii_geophysics_planetology_peer2013.pdf (439.06 KB) More Documents & Publications Blind Geothermal System Exploration in Active Volcanic Environments; Multi-phase Geophysical and

  10. Global climate change and maize production in Spain: Risk assessment and impacts of weather on yields

    SciTech Connect (OSTI)

    Feijoo, M.L.; Mestre, F.; Iglesias, A.; Rosenzweig, C.

    1996-12-31

    The study evaluates the potential effect of climate change on maize production in Spain, combining climate models, a crop productivity model, a decision support system and a yield-response model. The study was carried out for two agricultural regions that include the largest areas of Spain where maize is grown as a high input crop. The paper combines the output from a crop model with different techniques of analysis. The scenarios used in this study were generated from the output of two General Circulation Models (GCMs): the Goddard Institute for Space Studies model (GISS) and the Canadian Climate Change Model (CCCM). The study also includes a preliminary evaluation of the potential changes in monetary returns taking into account the possible variability of grain yields and prices, using mean-Gini stochastic dominance (MGSD). A yield response model was estimated using simulated data from the crop model. Weather variables are included. Typically, temperature and precipitation are the only weather variables included in these models. However, solar radiation is another important climate factor for plant growth and development and were included in the yield response model.

  11. A proposal to improve ecological compensation practice in road and railway projects in Spain

    SciTech Connect (OSTI)

    Villarroya, Ana Puig, Jordi

    2013-09-15

    To reduce ecological impacts caused by development projects, avoidance, minimization and compensation techniques have to be taken together into consideration along Environmental Impact Assessment (EIA) procedures. This paper explores the particular role that ecological compensation has had in recent road and railway EIA procedures in Spain, as seen through the review of a set of recent EIA Records of Decision (RODs) that confirms precedent findings. Noticing that residual impacts are not paid much attention, and that there is no evidence of a solid public participation in ecological impact evaluation, it proposes to increase the awareness on residual impacts, as a way to make easier public access to the allegedly most sensitive moment of EIA implementation: (residual) impact evaluation. -- Highlights: ► Ecological compensation practice in Spain is much lower than avoidance or mitigation. ► Residual impacts are overlooked in EIA processes and public participation is low. ► An increased awareness of residual impacts may also promote public participation. ► Current context needs these small steps to move towards better compensation practice.

  12. Lessons learned from the Siting Process of an Interim Storage Facility in Spain - 12024

    SciTech Connect (OSTI)

    Lamolla, Meritxell Martell

    2012-07-01

    On 29 December 2009, the Spanish government launched a site selection process to host a centralised interim storage facility for spent fuel and high-level radioactive waste. It was an unprecedented call for voluntarism among Spanish municipalities to site a controversial facility. Two nuclear municipalities, amongst a total of thirteen municipalities from five different regions, presented their candidatures to host the facility in their territories. For two years the government did not make a decision. Only in November 30, 2011, the new government elected on 20 November 2011 officially selected a non-nuclear municipality, Villar de Canas, for hosting this facility. This paper focuses on analysing the factors facilitating and hindering the siting of controversial facilities, in particular the interim storage facility in Spain. It demonstrates that involving all stakeholders in the decision-making process should not be underestimated. In the case of Spain, all regional governments where there were candidate municipalities willing to host the centralised interim storage facility, publicly opposed to the siting of the facility. (author)

  13. Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability presentation at the April 2013 peer review meeting held in Denver, Colorado. PDF icon hawaiigeophysicsplane...

  14. DOE ARM SP2 Deployment at Boston College_Aerodyne led Coated...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... The population mixing states were measured using the SP2 and ScanningTransmission ... reactor (providing controlled oxidative aging of such particles via OH and O 3 reactions ...

  15. FY 2015 Senior Executive Service (SES) and Senior Professional (SP) Performance Appraisal Opening Guidance

    Broader source: Energy.gov [DOE]

    The FY 2015 performance appraisal cycle for both SES and SP members, which includes Senior Level (SL) and Scientific or Professional (ST) employees, concludes on September 30, 2015.

  16. High-quality permanent draft genome sequence of Bradyrhizobium sp. strain WSM1743 - an effective microsymbiont of an Indigofera sp. growing in Australia

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Eshraghi, Leila; De Meyer, Sofie E.; Tian, Rui; Seshadri, Rekha; Ivanova, Natalia; Pati, Amrita; Markowitz, Victor; Woyke, Tanja; Kyrpides, Nikos C.; Tiwari, Ravi; et al

    2015-10-26

    Bradyrhizobium sp. strain WSM1743 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of an Indigofera sp. WSM1743 was isolated from a nodule recovered from the roots of an Indigofera sp. growing 20 km north of Carnarvon in Australia. It is slow growing, tolerates up to 1 % NaCl and is capable of growth at 37 °C. Here we describe the features of Bradyrhizobium sp. strain WSM1743, together with genome sequence information and its annotation. Finally, the 8,341,956 bp high-quality permanent draft genome is arranged into 163 scaffolds and 167more » contigs, contains 7908 protein-coding genes and 75 RNA-only encoding genes and was sequenced as part of the Root Nodule Bacteria chapter of the Genomic Encyclopedia of Bacteria and Archaea project.« less

  17. Evaluation of models for estimating solar irradiation on vertical surfaces at Valencia, Spain

    SciTech Connect (OSTI)

    Utrillas, M.P.; Martinez-Lozano, J.A.; Casanovas, A.J. )

    1991-01-01

    Hourly irradiation data recorded on vertical surfaces at north, east, south, and west orientations during the winter period going from December 1989 to March 1990 in Valencia, Spain, have been compared with estimated solar irradiation from several tilted-surface models. The isotropic-, Temps' and Coulson's-, Klucher's-, Hay's-, Shartveit's and Olseth's, Gueymard's-, and Perez' (simplified) models have been considered for this comparison. Root-mean-square-difference (RMSD), mean-bias-difference (MBD) and mean-absolute-difference (MAD) estimators have been used to measure the departure of models from experimental data. Modeled values are evaluated with the original coefficients proposed by the authors. Results of comparison show that the south orientation is less prone to modeling errors. Perez' 25{degree} circumsolar simplified model being the less affected by errors in north, eat and south orientations; for west orientation Klucher's model gives a lesser error. These results are consistent with those reported in previous studies.

  18. PAH bioconcentration in Mytilus sp from Sinclair Inlet, WA

    SciTech Connect (OSTI)

    Frazier, J.; Young, D.; Ozretich, R.; Echols, S.

    1995-12-31

    Approximately 20 polynuclear aromatic hydrocarbons (PAH) were measured by GC/MS in seawater and whole soft tissues of the intertidal mussel Mytilus sp. collected in July 1991 within and around Puget Sound`s Sinclair Inlet. Low variability was observed in the water concentrations collected over three days at control sites, yielding reliable values for the exposure levels experienced by this bioindicator mollusk. Mean water concentrations of acenaphthene, phenanthrene, and fluoranthene in the control region were 2.7 {+-} 0.8, 2.8 {+-} 0.8, and 3.1 {+-} 0.7 ng/liter, respectively. Levels measured near sites of vessel activity were higher but much more variable; this reduced the reliability of the tissue/water bioconcentration factors (BCF) obtained from these samples. An empirical model relating values of Log BCF and Log Kow for the control zone samples supports the utility of this estuarine bioindicator for monitoring general levels of PAH in nearshore surface waters.

  19. Complete genome sequence of Arthrobacter sp. strain FB24

    SciTech Connect (OSTI)

    Nakatsu, C. H.; Barabote, Ravi; Thompson, Sue; Bruce, David; Detter, Chris; Brettin, T.; Han, Cliff F.; Beasley, Federico; Chen, Weimin; Konopka, Allan; Xie, Gary

    2013-09-30

    Arthrobacter sp. strain FB24 is a species in the genus Arthrobacter Conn and Dimmick 1947, in the family Micrococcaceae and class Actinobacteria. A number of Arthrobacter genome sequences have been completed because of their important role in soil, especially bioremediation. This isolate is of special interest because it is tolerant to multiple metals and it is extremely resistant to elevated concentrations of chromate. The genome consists of a 4,698,945 bp circular chromosome and three plasmids (96,488, 115,507, and 159,536 bp, a total of 5,070,478 bp), coding 4,536 proteins of which 1,257 are without known function. This genome was sequenced as part of the DOE Joint Genome Institute Program.

  20. Nuclear facility licensing, documentaion, and reviews, and the SP-100 test site experience

    SciTech Connect (OSTI)

    Cornwell, B.C.; Deobald, T.L.; Bitten, E.J.

    1991-06-01

    The required approvals and permits to test a nuclear facility are extensive. Numerous regulatory requirements result in the preparation of documentation to support the approval process. The principal regulations for the SP-100 Ground Engineering System (GES) include the National Environmental Policy Act, Clean Air Act, and Atomic Energy Act. The documentation prepared for the SP-100 Nuclear Assembly Test (NAT) included an Environmental Assessment, state permit applications, and Safety Analysis Reports. This paper discusses the regulation documentation requirements and the SP-100 NAT Test Site experience. 12 refs., 2 figs., 2 tabs.

  1. Development and operativity of a real-time radiological monitoring network centered on the nuclear power plant of Almaraz (Spain)

    SciTech Connect (OSTI)

    Baeza, A.; Miro, C.; Puerto, J.A. del; Rio, M. del; Ortiz, F.; Paniagua, J.M.

    1993-12-01

    This work presents the hardware and software characteristics of the environmental surveillance radiological network that has been installed around the nuclear power station of Almaraz (Spain). A description is given of the program RADLINE which allows radiological data to be logged in real time, and a study is made of the operativity of the network and the methodology followed in establishing the radiological pre-alert and alert levels.

  2. An analysis of markets for small-scale, advanced coal-combustion technology in Spain, Italy, and Turkey

    SciTech Connect (OSTI)

    Not Available

    1989-09-01

    This report describes the results of an in-depth analysis of markets for US-developed, advanced coal-combustion technology (ACT) in the residential, commercial, and industrial sectors of three countries -- Spain, Italy, and Turkey. These countries were chosen in a previous study, in which member countries of the Organization for Economic Cooperation and Development (OECD) were rated on eight factors influencing their propensity to use small-scale, US-developed ACT. 76 refs., 16 figs., 14 tabs.

  3. FY 2014 Senior Executive Service (SES) and Senior Professional (SP) Performance Appraisal Cycle- Closeout Guidance

    Broader source: Energy.gov [DOE]

    The FY 2014 performance appraisal cycle for both SES and SP members, which includes Senior Level (SL) and Scientific or Professional (ST) employees, concludes on September 30, 2014.  In order to...

  4. Cyanobacterium sp. host cell and vector for production of chemical compounds in Cyanobacterial cultures

    DOE Patents [OSTI]

    Piven, Irina; Friedrich, Alexandra; Duhring, Ulf; Uliczka, Frank; Baier, Kerstin; Inaba, Masami; Shi, Tuo; Wang, Kui; Enke, Heike; Kramer, Dan

    2016-04-19

    A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

  5. ARM - Field Campaign - SP2 Deployment at Boston College-Aerodyne...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Deployment at Boston College-Aerodyne led Coated Black Carbon Study (BC4) ARM Data ... Campaign : SP2 Deployment at Boston College-Aerodyne led Coated Black Carbon Study (BC4) ...

  6. The conjugate gradient NAS parallel benchmark on the IBM SP1

    SciTech Connect (OSTI)

    Trefethen, A.E.; Zhang, T.

    1994-12-31

    The NAS Parallel Benchmarks are a suite of eight benchmark problems developed at the NASA Ames Research Center. They are specified in such a way that the benchmarkers are free to choose the language and method of implementation to suit the system in which they are interested. In this presentation the authors will discuss the Conjugate Gradient benchmark and its implementation on the IBM SP1. The SP1 is a parallel system which is comprised of RS/6000 nodes connected by a high performance switch. They will compare the results of the SP1 implementation with those reported for other machines. At this time, such a comparison shows the SP1 to be very competitive.

  7. Cyanobacterium sp. host cell and vector for production of chemical compounds in cyanobacterial cultures

    SciTech Connect (OSTI)

    Piven, Irina; Friedrich, Alexandra; Duhring, Ulf; Uliczka, Frank; Baier, Kerstin; Inaba, Masami; Shi, Tuo; Wang, Kui; Enke, Heike; Kramer, Dan

    2014-09-30

    A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.

  8. Energia Servizi Engineering SpA E S Engineering | Open Energy...

    Open Energy Info (EERE)

    SpA (E&S Engineering) Place: Rome, Italy Sector: Hydro, Wind energy Product: Italian developer of biogas, wind, mini-hydro and PV projects. Coordinates: 41.90311,...

  9. SP2 Deployment at Boston College-Aerodyne-Led Coated Black Carbon...

    Office of Scientific and Technical Information (OSTI)

    Coated Black Carbon Study (BC4) Final Campaign Summary Citation Details In-Document Search Title: SP2 Deployment at Boston College-Aerodyne-Led Coated Black Carbon Study (BC4) ...

  10. Fractionation of heavy metals in sludge from anaerobic wastewater stabilization ponds in southern Spain

    SciTech Connect (OSTI)

    Alonso, E.

    2006-07-01

    The analysis of heavy metals is a very important task to assess the potential environmental and health risk associated with the sludge coming from wastewater treatment plants (WWTPs). However, it is necessary to apply sequential extraction techniques to obtain suitable information about their bioavailability or toxicity. In this paper, a sequential extraction scheme according to the Standard, Measurements and Testing Programme of the European Commission was applied to sludge samples collected from ten anaerobic wastewater stabilization ponds (WSPs) located in southern Spain. Al, Cd, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Ti and Zn were determined in the sludge extracts by inductively coupled plasma atomic emission spectrometry. In relation to current international legislation for the use of sludge for agricultural purposes, none of the metal concentrations exceeded maximum permitted levels. Overall, heavy metals were mainly associated with the two less-available fractions (34% oxidizable metal and 55% residual metal). Only Mn and Zn showed the highest share of the available (exchangeable and reducible) fractions (25-48%)

  11. Road-corridor planning in the EIA procedure in Spain. A review of case studies

    SciTech Connect (OSTI)

    Loro, Manuel [Department of Urban and Regional Planning and Environment, Civil Engineering School, Universidad Politcnica de Madrid, Prof. Aranguren s Transport Research Centre Universidad Politcnica de Madrid, ETSI Caminos, Canales y Puertos, Prof. Aranguren s Centro de investigacin del transporte, TRANSyT-UPM, ETSI Caminos, Canales y Puertos, Universidad Politcnica de Madrid, Prof. Aranguren s Arce, Rosa M. [Department of Urban and Regional Planning and Environment, Civil Engineering School, Universidad Politcnica de Madrid, Prof. Aranguren s Transport Research Centre Universidad Politcnica de Madrid, ETSI Caminos, Canales y Puertos, Prof. Aranguren s Centro de investigacin del transporte, TRANSyT-UPM, ETSI Caminos, Canales y Puertos, Universidad Politcnica de Madrid, Prof. Aranguren s Ortega, Emilio [Transport Research Centre Universidad Politcnica de Madrid, ETSI Caminos, Canales y Puertos, Prof. Aranguren s Centro de investigacin del transporte, TRANSyT-UPM, ETSI Caminos, Canales y Puertos, Universidad Politcnica de Madrid, Prof. Aranguren s Department of Construction and Rural Roads, Forestry Engineering School, Universidad Politcnica de Madrid, Ciudad Universitaria s and others

    2014-01-15

    The assessment of different alternatives in road-corridor planning must be based on a number of well-defined territorial variables that serve as decision making criteria, and this requires a high-quality preliminary environmental assessment study. In Spain the formal specifications for the technical requirements stipulate the constraints that must be considered in the early stages of defining road corridors, but not how they should be analyzed and ranked. As part of the feasibility study of a new road definition, the most common methodology is to establish different levels of Territorial Carrying Capacity (TCC) in the study area in order to summarize the territorial variables on thematic maps and to ease the tracing process of road-corridor layout alternatives. This paper explores the variables used in 22 road-construction projects conducted by the Ministry of Public Works that were subject to the Spanish EIA regulation and published between 2006 and 2008. The aim was to evaluate the quality of the methods applied and the homogeneity and suitability of the variables used for defining the TCC. The variables were clustered into physical, environmental, land-use and cultural constraints for the purpose of comparing the TCC values assigned in the studies reviewed. We found the average quality of the studies to be generally acceptable in terms of the justification of the methodology, the weighting and classification of the variables, and the creation of a synthesis map. Nevertheless, the methods for assessing the TCC are not sufficiently standardized; there is a lack of uniformity in the cartographic information sources and methodologies for the TCC valuation. -- Highlights: We explore 22 road-corridor planning studies subjected to the Spanish EIA regulation. We analyze the variables selected for defining territorial carrying capacity. The quality of the studies is acceptable (methodology, variable weighting, mapping). There is heterogeneity in the methods for

  12. Bio sorption of strontium from aqueous solution by New Strain Bacillus sp. GTG-83

    SciTech Connect (OSTI)

    Tajer Mohammad Ghazvini, P.; Ghorbanzadeh Mashkani, S.; Ghafourian, H.

    2007-07-01

    Attempt was made to isolate bacterial strains capable of removing Sr biologically. In this study we collected ten different water samples from naturally radioactive spring Neydasht in Iran and bacterial strains samples isolated. Initial screening of a total of 50 bacterial isolates resulted in selection of one strain. The strain showed maximum adsorption capacity with 55 mg Sr/g dry wt. It was tentatively identified as Bacillus sp. according to morphological and biochemical properties and called strain GTG-83. Studies indicated that Bacillus sp. GTG-83 was able to grow aerobically in the presence of 50 mM SrCl{sub 2} but showed severe growth inhibition at levels above that concentration. The bio-sorption capacity of Bacillus sp. GTG-83 strongly depends on solution pH, and the maximum Sr sorption capacity of Bacillus sp. GTG-83 were obtained at pH 10 independent of the absence or the presence of increasing concentrations of salt (MgCl{sub 2}). Sr-salt bio-sorption studies were also performed at this pH values. Equilibrium uptakes of Sr increased with increasing Sr concentrations up to 250 mg/l for Bacillus sp. GTG-83. Maximum bio-sorption of Sr was obtained at temperatures in the range of 30-35 deg. C. Bacillus sp. GTG-83 bio-sorbed 97 mg Sr/g dry wt at 100 mg/l initial Sr concentration without salt medium (MgCl{sub 2}). When salt concentration (MgCl{sub 2}) increased to 15% (w/v), these values dropped to 23.6 mg Sr/g dry wt at the same conditions. Uptake of Sr within 5 min of incubation was relatively rapid and the absorption continued slowly thereafter. (authors)

  13. SP-100 planetary mission/system preliminary design study. Final report, technical information report

    SciTech Connect (OSTI)

    Jones, R.M.

    1986-02-01

    This report contains a discussion on many aspects of a nuclear electric propulsion planetary science mission and spacecraft using the proposed SP-100 nuclear power subsystem. A review of the science rationale for such missions is included. A summary of eleven nuclear electric propulsion planetary missions is presented. A conceptual science payload, mission design, and spacecraft design is included for the Saturn Ring Rendezvous mission. Spacecraft and mission costs have been estimated for two potential sequences of nuclear electric propulsion planetary missions. The integration issues and requirements on the proposed SP-100 power subsystems are identified.

  14. ARM - Field Campaign - Colorado: SP2 Deployment at StormVEx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SP2 Deployment at StormVEx ARM Data Discovery Browse Data Related Campaigns Colorado: The Storm Peak Lab Cloud Property Validation Experiment (STORMVEX) 2010.11.15, Mace, AMF Comments? We would love to hear from you! Send us a note below or call us at 1-888-ARM-DATA. Send Campaign : Colorado: SP2 Deployment at StormVEx 2010.11.15 - 2011.04.25 Lead Scientist : Arthur Sedlacek For data sets, see below. Abstract How does the boundary layer influence BC encapsulation? Is there a correlation between

  15. Cadmium exposure and neuropsychological development in school children in southwestern Spain

    SciTech Connect (OSTI)

    Rodríguez-Barranco, Miguel; Lacasaña, Marina; Gil, Fernando; Lorca, Andres; Alguacil, Juan; Rohlman, Diane S.; González-Alzaga, Beatriz; Molina-Villalba, Isabel; Mendoza, Ramón; Aguilar-Garduño, Clemente

    2014-10-15

    This study assessed the association between cadmium exposure and neuropsychological development in children from a region with high industrial and mining activities in southwestern Spain. We conducted a cross-sectional study with 261 children aged 6–9 years between January and March 2012. Cadmium exposure was measured in urine and hair of children, and neuropsychological development was assessed with the Wechsler Intelligence Scale for Children-Fourth Edition (WISC-IV) and with three computerized tests from the Behavioral Assessment and Research System (BARS): Reaction Time Test (RTT), Continuous Performance Test (CPT) and Selective Attention Test (SAT). Multivariate linear regression models, adjusted for potential confounders, were used to estimate the association between neuropsychological development and cadmium exposure measured in urine and hair samples. Geometric means of urine and hair cadmium levels were 0.75 μg/g creatinine and 0.01 μg/g, respectively. We observed that doubling of levels of cadmium in urine was associated with a reduction of two points (95% CI: −3.8 to −0.4) in the Full-Scale intelligence quotient (IQ) in boys. By domains, association was statistically significant for Verbal Comprehension (β=−2.0; p=0.04) and close to the significance level for Perceptual Reasoning (β=−1.8; p=0.06). Among girls, only Verbal Comprehension showed suggestive associations with cadmium exposure (β=−1.7; p=0.06). Cadmium exposure is associated with cognitive delays in boys in our region. Our results provide additional evidence of the neurotoxic effect of low-level postnatal cadmium exposure among children, and support the hypothesis of differences between sexes in the neurotoxic effect of metals on children. - Highlights: • This study associates Cd exposure and neuropsychological development in children. • Cd exposure was associated with cognitive delay in boys, but not in girls. • Intellectual quotient of boys decreased two points for a

  16. Heavy metal transfers between trophic compartments in different ecosystems in Galicia (Northwest Spain): Essential elements

    SciTech Connect (OSTI)

    Gonzalez, X.I.; Aboal, J.R.; Fernandez, J.A.; Carballeira, A.

    2008-11-15

    In the present study, we determined the concentrations of Cu, Fe, Mn, and Zn in soil and several trophic compartments at a total of 16 sampling stations. The trophic compartments studied were primary producers, represented by two species of terrestrial mosses (Pseudoescleropodium purum and Hypnum cupressiforme) and oak trees (Quercus robur or Q. pyrenaica); primary consumers, represented by the wood mouse (Apodemus sylvaticus) and the yellow necked mouse (A. flavicollis); secondary consumers, represented by the shrew (Sorex granarius); and finally, detritivores, represented by slugs (Arion ater). Thirteen of the sampling stations were located in mature oak woodlands (Quercus sp.); two of the sampling stations were located in the area surrounding a restored lignite mine dump, and the other in an ultrabasic area. The analytical determinations revealed a lack of significant correlations among trophic compartments, possibly caused by effective regulation of metals by organisms and/or spatial variation in availability of metals from soil or food. Furthermore, the only element that showed a clear pattern of biomagnification was Cu; as for the other elements, there was always some divergence from such a pattern. Finally, the patterns of bioaccumulation in contaminated and woodland sampling stations were very similar, although there was enrichment of the concentrations of Cu, Mn, and Zn in the mice viscera, which, except for Mn, were related to higher edaphic concentrations.

  17. An analysis of markets for small-scale, advanced coal-combustion technology in Spain, Italy, and Turkey

    SciTech Connect (OSTI)

    Placet, M.; Gerry, P.A.; Kenski, D.M.; Kern, D.M.; Nehring, J.L.; Szpunar, C.B.

    1989-09-01

    This report discusses the examination of potential overseas markets for using small-scale, US-developed, advanced coal-combustion technologies (ACTs). In previous work, member countries of the Organization for Economic Cooperation and Development (OECD) were rated on their potential for using ACTs through a comprehensive screening methodology. The three most promising OECD markets were found to be Spain, Italy, and Turkey. This report provides in-depth analyses of these three selected countries. First, it addresses changes in the European Community with particular reference to the 1992 restructuring and its potential effect on the energy situation in Europe, specifically in the three subject countries. It presents individual country studies that examine demographics, economics, building infrastructures, and energy-related factors. Potential niches for ACTs are explored for each country through regional analyses. Marketing channels, strategies, and the trading environments in each country are also discussed. The information gathered indicates that Turkey is a most promising market, Spain is a fairly promising market, and Italy appears to be a somewhat limited market for US ACTs. 76 refs., 16 figs., 14 tabs.

  18. Genome Sequence of the Alkaline-Tolerant Cellulomonas sp. Strain FA1

    SciTech Connect (OSTI)

    Cohen, Michael F.; Hu, Ping; Nguyen, My Vu; Kamennaya, Nina; Brown, Natasha; Woyke, Tanja; Kyrpides, Nikos; Holman, Hoi-Ying; Torok, Tamas

    2015-06-18

    We present the genome of the cellulose-degrading Cellulomonas sp. strain FA1 isolated from an actively serpentinizing highly alkaline spring. Knowledge of this genome will enable studies into the molecular basis of plant material degradation in alkaline environments and inform the development of lignocellulose bioprocessing procedures for biofuel production.

  19. Genome Sequence of Amycolatopsis sp Strain ATCC 39116, a Plant Biomass-Degrading Actinomycete

    SciTech Connect (OSTI)

    Davis, Jennifer R.; Goodwin, Lynne A.; Woyke, Tanja; Teshima, Hazuki; Bruce, David; Detter, J. Chris; Tapia, Roxanne; Han, Shunsheng; Han, James; Pitluck, Sam; Nolan, Matt; Mikhailova, Natalia; Land, Miriam L; Sello, Jason K.

    2012-01-01

    We announce the availability of a high-quality draft of the genome sequence of Amycolatopsis sp. strain 39116, one of few bacterial species that are known to consume the lignin component of plant biomass. This genome sequence will further ongoing efforts to use microorganisms for the conversion of plant biomass into fuels and high-value chemicals.

  20. Overview of the 1985 NASA Lewis Research Center SP-100 free-piston Stirling engine activities

    SciTech Connect (OSTI)

    Slaby, J.G.

    1985-01-01

    An overview of the 1985 (NASA) Lewis Research Center free-piston Stirling engine activities in support of the SP-100 Program is presented. The SP-100 program is being conducted in support of the Department of Advanced Research Projects Agency (DARPA) and the Department of Energy (DOE), and NASA. This effort is keyed on the design, fabrication, assembly, and testing of a 25 kW(e) Stirling space-power technology-feasibility demonstrator engine. Another facet of the SP-100 project covers the status of a 9000-h goal endurance test conducted on a 2 kW(e) free-piston Stirling/linear alternator system employing hydrostatic gas bearings. Dynamic balancing of the RE-1000 engine (a 1 kW(e) free-piston Stirling engine) using a passive dynamic absorber is discussed, along with the results of a parametric study showing the relationships of Stirling power converter specific weight and efficiency as functions of Stirling engine heater to cooler temperature ratio. Planned tests are described covering a hydrodynamic gas bearing concept for potential SP-100 application.

  1. Genome Sequence of the Alkaline-Tolerant Cellulomonas sp. Strain FA1

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cohen, Michael F.; Hu, Ping; Nguyen, My Vu; Kamennaya, Nina; Brown, Natasha; Woyke, Tanja; Kyrpides, Nikos; Holman, Hoi-Ying; Torok, Tamas

    2015-06-18

    We present the genome of the cellulose-degrading Cellulomonas sp. strain FA1 isolated from an actively serpentinizing highly alkaline spring. Knowledge of this genome will enable studies into the molecular basis of plant material degradation in alkaline environments and inform the development of lignocellulose bioprocessing procedures for biofuel production.

  2. AmeriFlux US-SP1 Slashpine-Austin Cary- 65yrs nat regen

    SciTech Connect (OSTI)

    Martin, Tim

    2016-01-01

    This is the AmeriFlux version of the carbon flux data for the site US-SP1 Slashpine-Austin Cary- 65yrs nat regen. Site Description - The ACMF site is a 67 hectare naturally regenerated Pinus palustris and Pinus elliottii mixed stand.

  3. Climatic implications of correlated upper Pleistocene glacial and fluvial deposits on the Cinca and Gallego rivers, NE Spain

    SciTech Connect (OSTI)

    Lewis, Claudia J; Mcdonald, Eric; Sancho, Carlos; Pena, Jose- Luis

    2008-01-01

    We correlate Upper Pleistocene glacial and fluvial deposits of the Cinca and Gallego River valleys (south central Pyrenees and Ebro basin, Spain) using geomorphic position, luminescence dates, and time-related trends in soil development. The ages obtained from glacial deposits indicate glacial periods at 85 {+-} 5 ka, 64 {+-} 11 ka, and 36 {+-} 3 ka (from glacial till) and 20 {+-} 3 ka (from loess). The fluvial drainage system, fed by glaciers in the headwaters, developed extensive terrace systems in the Cinca River valley at 178 {+-} 21 ka, 97 {+-} 16 ka, 61 {+-} 4 ka, 47 {+-} 4 ka, and 11 {+-} 1 ka, and in the Gallego River valley at 151 {+-} 11 ka, 68 {+-} 7 ka, and 45 {+-} 3 ka. The times of maximum geomorphic activity related to cold phases coincide with Late Pleistocene marine isotope stages and heinrich events. The maximum extent of glaciers during the last glacial occurred at 64 {+-} 11 ka, and the terraces correlated with this glacial phase are the most extensive in both the Cinca (61 {+-} 4 ka) and Gallego (68 {+-} 7 ka) valleys, indicating a strong increase in fluvial discharge and availability of sediments related to the transition to deglaciation. The global Last Glacial Maximum is scarcely represented in the south central Pyrenees owing to dominantly dry conditions at that time. Precipitation must be controlled by the position of the Iberian Peninsula with respect to the North Atlantic atmospheric circulation system. The glacial systems and the associated fluvial dynamic seem sensitive to (1) global climate changes controlled by insolation, (2) North Atlantic thermohaline circulation influenced by freshwater pulses into the North Atlantic, and (3) anomalies in atmospheric circulation in the North Atlantic controlling precipitation on the Iberian peninsula. The model of glacial and fluvial evolution during the Late Pleistocene in northern Spain could be extrapolated to other glaciated mountainous areas in southern Europe.

  4. Environmental assessment of SP-100 ground engineering system test site: Hanford Site, Richland, Washington

    SciTech Connect (OSTI)

    Not Available

    1988-12-01

    The US Department of Energy (DOE) proposes to modify an existing reactor containment building (decommissioned Plutonium Recycle Test Reactor (PRTR) 309 Building) to provide ground test capability for the prototype SP-100 reactor. The 309 Building (Figure 1.1) is located in the 300 Area on the Hanford Site in Washington State. The National Environmental Policy Act (NEPA) requires that Federal agencies assess the potential impacts that their actions may have on the environment. This Environmental Assessment describes the consideration given to environmental impacts during reactor concept and test site selection, examines the environmental effects of the DOE proposal to ground test the nuclear subsystem, describes alternatives to the proposed action, and examines radiological risks of potential SP-100 use in space. 73 refs., 19 figs., 7 tabs.

  5. PySP : modeling and solving stochastic mixed-integer programs in Python.

    SciTech Connect (OSTI)

    Woodruff, David L.; Watson, Jean-Paul

    2010-08-01

    Although stochastic programming is a powerful tool for modeling decision-making under uncertainty, various impediments have historically prevented its widespread use. One key factor involves the ability of non-specialists to easily express stochastic programming problems as extensions of deterministic models, which are often formulated first. A second key factor relates to the difficulty of solving stochastic programming models, particularly the general mixed-integer, multi-stage case. Intricate, configurable, and parallel decomposition strategies are frequently required to achieve tractable run-times. We simultaneously address both of these factors in our PySP software package, which is part of the COIN-OR Coopr open-source Python project for optimization. To formulate a stochastic program in PySP, the user specifies both the deterministic base model and the scenario tree with associated uncertain parameters in the Pyomo open-source algebraic modeling language. Given these two models, PySP provides two paths for solution of the corresponding stochastic program. The first alternative involves writing the extensive form and invoking a standard deterministic (mixed-integer) solver. For more complex stochastic programs, we provide an implementation of Rockafellar and Wets Progressive Hedging algorithm. Our particular focus is on the use of Progressive Hedging as an effective heuristic for approximating general multi-stage, mixed-integer stochastic programs. By leveraging the combination of a high-level programming language (Python) and the embedding of the base deterministic model in that language (Pyomo), we are able to provide completely generic and highly configurable solver implementations. PySP has been used by a number of research groups, including our own, to rapidly prototype and solve difficult stochastic programming problems.

  6. Direct synthesis of sp-bonded carbon chains on graphite surface by femtosecond laser irradiation

    SciTech Connect (OSTI)

    Hu, A.; Rybachuk, M.; Lu, Q.-B.; Duley, W. W.

    2007-09-24

    Microscopic phase transformation from graphite to sp-bonded carbon chains (carbyne) and nanodiamond has been induced by femtosecond laser pulses on graphite surface. UV/surface enhanced Raman scattering spectra and x-ray photoelectron spectra displayed the local synthesis of carbyne in the melt zone while nanocrystalline diamond and trans-polyacetylene chains form in the edge area of gentle ablation. These results evidence possible direct 'writing' of variable chemical bonded carbons by femtosecond laser pulses for carbon-based applications.

  7. Metal Catalyzed sp2 Bonded Carbon - Large-scale Graphene Synthesis and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Beyond | MIT-Harvard Center for Excitonics Metal Catalyzed sp2 Bonded Carbon - Large-scale Graphene Synthesis and Beyond December 1, 2009 at 3pm/36-428 Peter Sutter Center for Functional Nanomaterials sutter abstract: Carbon honeycomb lattices have shown a number of remarkable properties. When wrapped up into fullerenes, for instance, superconductivity with high transition temperatures can be induced by alkali intercalation. Rolling carbon sheets up into 1-dimensional nanotubes generates the

  8. Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Spectral SP: A New Approach to Mapping Reservoir Flow and Permeability Donald Thomas University of Hawaii Hawaii Institute of Geophysics and Planetology Research and Development/Geophysics Project Officer: Mark Ziegenbein Total Project Funding: $ $1,106,271 April 24, 2013 This presentation does not contain any proprietary confidential, or otherwise restricted information. 2 | US DOE Geothermal Office eere.energy.gov Relevance/Impact of Research Statement of the Problem * Surface exploration

  9. Gas production and behavior in the coolant of the SP-100 Space Nuclear Power System

    SciTech Connect (OSTI)

    McGhee, J.M.

    1989-08-01

    The radiologic generation and subsequent behavior of helium gas in the lithium coolant of SP-100 class space nuclear power reactors was investigated analytically in a two part study. Part One of the study consisted of a calculation of coolant radiologic helium gas production rates in a SP-100 class reactor using the discrete ordinates code TWODANT. Cross sections were developed from ENDF/B-V data via the MATXS6s master cross section library. Cross sections were self shielded assuming one homogeneous core region, and doppler broadened to 1300 K using the cross section preparation code TRANSX. Calculations were performed using an S{sub 4}/P{sub 1} approximation and 80 neutron energy groups. Part Two of the study consisted of a theoretical investigation into the behavior of helium gas in the primary loop of lithium cooled space reactors. The SP-100 space power system was used as a representative of such a system. Topics investigated included: (1) heterogeneous and homogeneous nucleation; (2) bubble growth/collapse by diffusion, mechanical temperature/pressure effects, and coalescence; and, (3) the effects on bubble distribution of microgravity, magnetic fields, and inertially induced buoyancy. 104 refs., 78 figs., 28 tabs.

  10. Complete genome sequences of Geobacillus sp. WCH70, a thermophilic strain isolated from wood compost

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Brumm, Phillip; Land, Miriam L.; Mead, David

    2016-04-27

    Geobacillus sp. WCH70 was one of several thermophilic organisms isolated from hot composts in the Middleton, WI area. Comparison of 16 S rRNA sequences showed the strain may be a new species, and is most closely related to G. galactosidasius and G. toebii. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2009 (CP001638). The genome of Geobacillus species WCH70 consists of one circular chromosome of 3,893,306 bp with an average G + C content of 43 %, and two circular plasmids of 33,899 and 10,287 bp with anmore » average G + C content of 40 %. Among sequenced organisms, Geobacillus sp. WCH70 shares highest Average Nucleotide Identity (86 %) with G. thermoglucosidasius strains, as well as similar genome organization. Geobacillus sp. WCH70 appears to be a highly adaptable organism, with an exceptionally high 125 annotated transposons in the genome. The organism also possesses four predicted restriction-modification systems not found in other Geobacillus species.« less

  11. Overview of free-piston Stirling SP-100 activities at the NASA Lewis Research Center

    SciTech Connect (OSTI)

    Slaby, J.G.

    1986-01-01

    An overview of the National Aeronautics and Space Administration (NASA) Lewis Research Center (LeRC) SP-100 free-piston Stirling engine activities is presented. These activities are being conducted in support of the Department of Defense (DOD), Department of Energy (DOE), and NASA. The space-power technology effort, under SP-100, addresses the status of the 25 kWe Space Power Demonstrator Engine (SPDE). Another facet of the SP-100 project covers the status of an endurance test. Dynamic balancing of the SPDE engine will be discussed along with a summary covering the parametric results of a study showing the relationship between power-converter specific weight and efficiency both as a function of Stirling engine heater to cooler temperature ratio. Design parameters and conceptual design features will be presented for a 25 kWe, single-cylinder free-piston Stirling space-power converter. And finally, a description of a hydrodynamic gas bearing concept will be presented.

  12. Upgraded biogas from municipal solid waste for natural gas substitution and CO{sub 2} reduction – A case study of Austria, Italy, and Spain

    SciTech Connect (OSTI)

    Starr, Katherine; Villalba, Gara; Gabarrell, Xavier

    2015-04-15

    Highlights: • Biogas can be upgraded to create biomethane, a substitute to natural gas. • Biogas upgrading was applied to landfills and anaerobic digestors in 3 countries. • Up to 0.6% of a country’s consumption of natural gas could be replaced by biomethane. • Italy could save 46% of the national CO{sub 2} emissions attributed to the waste sector. • Scenarios were created to increase biomethane production. - Abstract: Biogas is rich in methane and can be further purified through biogas upgrading technologies, presenting a viable alternative to natural gas. Landfills and anaerobic digestors treating municipal solid waste are a large source of such biogas. They therefore offer an attractive opportunity to tap into this potential source of natural gas while at the same time minimizing the global warming impact resulting from methane emissions in waste management schemes (WMS) and fossil fuel consumption reduction. This study looks at the current municipal solid waste flows of Spain, Italy, and Austria over one year (2009), in order to determine how much biogas is generated. Then it examines how much natural gas could be substituted by using four different biogas upgrading technologies. Based on current waste generation rates, exploratory but realistic WMS were created for each country in order to maximize biogas production and potential for natural gas substitution. It was found that the potential substitution of natural gas by biogas resulting from the current WMS seems rather insignificant: 0.2% for Austria, 0.6% for Italy and 0.3% for Spain. However, if the WMS is redesigned to maximize biogas production, these figures can increase to 0.7% for Austria, 1% for Italy and 2% for Spain. Furthermore, the potential CO{sub 2} reduction as a consequence of capturing the biogas and replacing fossil fuel can result in up to a 93% reduction of the annual national waste greenhouse gas emissions of Spain and Italy.

  13. Roof-top solar energy potential under performance-based building energy codes: The case of Spain

    SciTech Connect (OSTI)

    Izquierdo, Salvador; Montanes, Carlos; Dopazo, Cesar; Fueyo, Norberto

    2011-01-15

    The quantification at regional level of the amount of energy (for thermal uses and for electricity) that can be generated by using solar systems in buildings is hindered by the availability of data for roof area estimation. In this note, we build on an existing geo-referenced method for determining available roof area for solar facilities in Spain to produce a quantitative picture of the likely limits of roof-top solar energy. The installation of solar hot water systems (SHWS) and photovoltaic systems (PV) is considered. After satisfying up to 70% (if possible) of the service hot water demand in every municipality, PV systems are installed in the remaining roof area. Results show that, applying this performance-based criterion, SHWS would contribute up to 1662 ktoe/y of primary energy (or 68.5% of the total thermal-energy demand for service hot water), while PV systems would provide 10 T W h/y of electricity (or 4.0% of the total electricity demand). (author)

  14. High-quality permanent draft genome sequence of the Parapiptadenia rigida-nodulating Burkholderia sp. strain UYPR1.413

    SciTech Connect (OSTI)

    De Meyer, Sofie E.; Fabiano, Elena; Tian, Rui; Van Berkum, Peter; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Howieson, John; Kyrpides, Nikos; Reeve, Wayne

    2015-06-04

    We report that Burkholderia sp. strain UYPR1.413 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Parapiptadenia rigida collected at the Angico plantation, Mandiyu, Uruguay, in December 2006. A survey of symbionts of P. rigida in Uruguay demonstrated that this species is nodulated predominantly by Burkholderia microsymbionts. Moreover, Burkholderia sp. strain UYPR1.413 is a highly efficient nitrogen fixing symbiont with this host. Currently, the only other sequenced isolate to fix with this host is Cupriavidus sp. UYPR2.512. Therefore, Burkholderia sp. strain UYPR1.413 was selected for sequencing on the basis of its environmental and agricultural relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the GEBA-RNB project. Here we describe the features of Burkholderia sp. strain UYPR1.413, together with sequence and annotation. The 10,373,764 bp high-quality permanent draft genome is arranged in 336 scaffolds of 342 contigs, contains 9759 protein-coding genes and 77 RNA-only encoding genes.

  15. High-quality permanent draft genome sequence of the Parapiptadenia rigida-nodulating Burkholderia sp. strain UYPR1.413

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    De Meyer, Sofie E.; Fabiano, Elena; Tian, Rui; Van Berkum, Peter; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; et al

    2015-06-04

    We report that Burkholderia sp. strain UYPR1.413 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Parapiptadenia rigida collected at the Angico plantation, Mandiyu, Uruguay, in December 2006. A survey of symbionts of P. rigida in Uruguay demonstrated that this species is nodulated predominantly by Burkholderia microsymbionts. Moreover, Burkholderia sp. strain UYPR1.413 is a highly efficient nitrogen fixing symbiont with this host. Currently, the only other sequenced isolate to fix with this host is Cupriavidus sp. UYPR2.512. Therefore, Burkholderia sp. strain UYPR1.413 was selected for sequencing on the basis of its environmental and agriculturalmore » relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the GEBA-RNB project. Here we describe the features of Burkholderia sp. strain UYPR1.413, together with sequence and annotation. The 10,373,764 bp high-quality permanent draft genome is arranged in 336 scaffolds of 342 contigs, contains 9759 protein-coding genes and 77 RNA-only encoding genes.« less

  16. Investigation of refractory black carbon-containing particle morphologies using the single-particle soot photometer (SP2)

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sedlacek, III, Arthur J.; Lewis, Ernie R.; Onasch, Timothy B.; Lambe, Andrew T.; Davidovits, Paul

    2015-07-24

    An important source of uncertainty in radiative forcing by absorbing aerosol particles is the uncertainty in their morphologies (i.e., the location of the absorbing substance on/in the particles). To examine the effects of particle morphology on the response of an individual black carbon-containing particle in a Single-Particle Soot Photometer (SP2), a series of experiments was conducted to investigate black carbon-containing particles of known morphology using Regal black (RB), a proxy for collapsed soot, as the light-absorbing substance. Particles were formed by coagulation of RB with either a solid substance (sodium chloride or ammonium sulfate) or a liquid substance (dioctyl sebacate),more » and by condensation with dioctyl sebacate, the latter experiment forming particles in a core-shell configuration. Each particle type experienced fragmentation (observed as negative lagtimes), and each yielded similar lagtime responses in some instances, confounding attempts to differentiate particle morphology using current SP2 lagtime analysis. SP2 operating conditions, specifically laser power and sample flow rate, which in turn affect the particle heating and dissipation rates, play an important role in the behavior of particles in the SP2, including probability of fragmentation. This behavior also depended on the morphology of the particles and on the thermo-chemical properties of the non-RB substance. Although these influences cannot currently be unambiguously separated, the SP2 analysis may still provide useful information on particle mixing states and black carbon particle sources.« less

  17. Investigation of refractory black carbon-containing particle morphologies using the single-particle soot photometer (SP2)

    SciTech Connect (OSTI)

    Sedlacek, III, Arthur J.; Lewis, Ernie R.; Onasch, Timothy B.; Lambe, Andrew T.; Davidovits, Paul

    2015-07-24

    An important source of uncertainty in radiative forcing by absorbing aerosol particles is the uncertainty in their morphologies (i.e., the location of the absorbing substance on/in the particles). To examine the effects of particle morphology on the response of an individual black carbon-containing particle in a Single-Particle Soot Photometer (SP2), a series of experiments was conducted to investigate black carbon-containing particles of known morphology using Regal black (RB), a proxy for collapsed soot, as the light-absorbing substance. Particles were formed by coagulation of RB with either a solid substance (sodium chloride or ammonium sulfate) or a liquid substance (dioctyl sebacate), and by condensation with dioctyl sebacate, the latter experiment forming particles in a core-shell configuration. Each particle type experienced fragmentation (observed as negative lagtimes), and each yielded similar lagtime responses in some instances, confounding attempts to differentiate particle morphology using current SP2 lagtime analysis. SP2 operating conditions, specifically laser power and sample flow rate, which in turn affect the particle heating and dissipation rates, play an important role in the behavior of particles in the SP2, including probability of fragmentation. This behavior also depended on the morphology of the particles and on the thermo-chemical properties of the non-RB substance. Although these influences cannot currently be unambiguously separated, the SP2 analysis may still provide useful information on particle mixing states and black carbon particle sources.

  18. Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Billings, Andrew F.; Fortney, Julian L.; Hazen, Terry C.; Simmons, Blake; Davenport, Karen W.; Goodwin, Lynne; Ivanova, Natalia; Kyrpides, Nikos C.; Mavromatis, Konstantinos; Woyke, Tanja; et al

    2015-11-19

    Tolumonas lignolytica BRL6-1T sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1T are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The genome for this isolate was sequenced and returned in seven unique contigs totaling 3.6Mbp, enabling the characterization of several putative pathways for lignin breakdown. Particularly, we found an extracellular peroxidase involved in lignin depolymerization, as well as several enzymes involvedmore » in β-aryl ether bond cleavage, which is the most abundant linkage between lignin monomers. We also found genes for enzymes involved in ferulic acid metabolism, which is a common product of lignin breakdown. Finally, by characterizing pathways and enzymes employed in the bacterial breakdown of lignin in anaerobic environments, this work should assist in the efficient engineering of biofuel production from lignocellulosic material.« less

  19. Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.

    SciTech Connect (OSTI)

    Billings, Andrew F.; Fortney, Julian L.; Hazen, Terry C.; Simmons, Blake; Davenport, Karen W.; Goodwin, Lynne; Ivanova, Natalia; Kyrpides, Nikos C.; Mavromatis, Konstantinos; Woyke, Tanja; DeAngelis, Kristen M.

    2015-11-19

    Tolumonas lignolytica BRL6-1T sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1T are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The genome for this isolate was sequenced and returned in seven unique contigs totaling 3.6Mbp, enabling the characterization of several putative pathways for lignin breakdown. Particularly, we found an extracellular peroxidase involved in lignin depolymerization, as well as several enzymes involved in β-aryl ether bond cleavage, which is the most abundant linkage between lignin monomers. We also found genes for enzymes involved in ferulic acid metabolism, which is a common product of lignin breakdown. Finally, by characterizing pathways and enzymes employed in the bacterial breakdown of lignin in anaerobic environments, this work should assist in the efficient engineering of biofuel production from lignocellulosic material.

  20. Genomes of three facultatively symbiotic Frankia sp. strainsreflect host plant biogeography

    SciTech Connect (OSTI)

    Normand, Philippe; Lapierre, Pascal; Tisa, Louis S.; Gogarten, J.Peter; Alloisio, Nicole; Bagnarol, Emilie; Bassi, Carla A.; Berry,Alison; Bickhart, Derek M.; Choisne, Nathalie; Couloux, Arnaud; Cournoyer, Benoit; Cruveiller, Stephane; Daubin, Vincent; Demange, Nadia; Francino, M. Pilar; Ggoltsman, Eugene; Huang, Ying; Kopp, Olga; Labarre,Laurent; Lapidus, Alla; Lavire, Celine; Marechal, Joelle; Martinez,Michele; Mastronunzio, Juliana E.; Mullin, Beth; Niemann, James; Pujic,Pierre; Rawnsley, Tania; Rouy, Zoe; Schenowitz, Chantal; Sellstedt,Anita; Tavares, Fernando; Tomkins, Jeffrey P.; Vallenet, David; Valverde,Claudio; Wall, Luis; Wang, Ying; Medigue, Claudine; Benson, David R.

    2006-02-01

    Filamentous actinobacteria from the genus Frankia anddiverse woody trees and shrubs together form N2-fixing actinorhizal rootnodule symbioses that are a major source of new soil nitrogen in widelydiverse biomes 1. Three major clades of Frankia sp. strains are defined;each clade is associated with a defined subset of plants from among theeight actinorhizal plant families 2,3. The evolution arytrajectoriesfollowed by the ancestors of both symbionts leading to current patternsof symbiont compatibility are unknown. Here we show that the competingprocesses of genome expansion and contraction have operated in differentgroups of Frankia strains in a manner that can be related to thespeciation of the plant hosts and their geographic distribution. Wesequenced and compared the genomes from three Frankia sp. strains havingdifferent host plant specificities. The sizes of their genomes variedfrom 5.38 Mbp for a narrow host range strain (HFPCcI3) to 7.50Mbp for amedium host range strain (ACN14a) to 9.08 Mbp for a broad host rangestrain (EAN1pec.) This size divergence is the largest yet reported forsuch closely related bacteria. Since the order of divergence of thestrains is known, the extent of gene deletion, duplication andacquisition could be estimated and was found to be inconcert with thebiogeographic history of the symbioses. Host plant isolation favoredgenome contraction, whereas host plant diversification favored genomeexpansion. The results support the idea that major genome reductions aswell as expansions can occur in facultatively symbiotic soil bacteria asthey respond to new environments in the context of theirsymbioses.

  1. High-quality permanent draft genome sequence of the Parapiptadenia rigida-nodulating Cupriavidus sp. strain UYPR2.512

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    De Meyer, Sofie E.; Fabiano, Elena; Tian, Rui; Van Berkum, Peter; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia N.; Pati, Amrita; Woyke, Tanja; et al

    2015-04-11

    Cupriavidus sp. strain UYPR2.512 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Parapiptadenia rigida grown in soils from a native forest of Uruguay. Here we describe the features of Cupriavidus sp. strain UYPR2.512, together with sequence and annotation. We find the 7,858,949 bp high-quality permanent draft genome is arranged in 365 scaffolds of 369 contigs, contains 7,411 protein-coding genes and 76 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.

  2. A reliability and mass perspective of SP-100 Stirling cycle lunar-base powerplant designs

    SciTech Connect (OSTI)

    Bloomfield, H.S.

    1991-06-01

    The purpose was to obtain reliability and mass perspectives on selection of space power system conceptual designs based on SP-100 reactor and Stirling cycle power-generation subsystems. The approach taken was to: (1) develop a criterion for an acceptable overall reliability risk as a function of the expected range of emerging technology subsystem unit reliabilities; (2) conduct reliability and mass analyses for a diverse matrix of 800-kWe lunar-base design configurations employing single and multiple powerplants with both full and partial subsystem redundancy combinations; and (3) derive reliability and mass perspectives on selection of conceptual design configurations that meet an acceptable reliability criterion with the minimum system mass increase relative to reference powerplant design. The developed perspectives provided valuable insight into the considerations required to identify and characterize high-reliability and low-mass lunar-base powerplant conceptual design.

  3. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  4. Additive synthesis with DIASS-M4C on Argonne National Laboratory`s IBM POWERparallel System (SP)

    SciTech Connect (OSTI)

    Kaper, H.; Ralley, D.; Restrepo, J.; Tiepei, S.

    1995-12-31

    DIASS-M4C, a digital additive instrument was implemented on the Argonne National Laboratory`s IBM POWER parallel System (SP). This paper discusses the need for a massively parallel supercomputer and shows how the code was parallelized. The resulting sounds and the degree of control the user can have justify the effort and the use of such a large computer.

  5. Draft genome sequence of Sulfurospirillum sp. strain MES, reconstructed from the metagenome of a microbial electrosynthesis system

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ross, Daniel E.; Marshall, Christopher W.; May, Harold D.; Norman, R. Sean

    2015-01-15

    A draft genome of Sulfurospirillum sp. strain MES was isolated through taxonomic binning of a metagenome sequenced from a microbial electrosynthesis system (MES) actively producing acetate and hydrogen. The genome contains the nosZDFLY genes, which are involved in nitrous oxide reduction, suggesting the potential role of this strain in denitrification.

  6. Draft genome sequence of Sulfurospirillum sp. strain MES, reconstructed from the metagenome of a microbial electrosynthesis system

    SciTech Connect (OSTI)

    Ross, Daniel E.; Marshall, Christopher W.; May, Harold D.; Norman, R. Sean

    2015-01-15

    A draft genome of Sulfurospirillum sp. strain MES was isolated through taxonomic binning of a metagenome sequenced from a microbial electrosynthesis system (MES) actively producing acetate and hydrogen. The genome contains the nosZDFLY genes, which are involved in nitrous oxide reduction, suggesting the potential role of this strain in denitrification.

  7. Draft genome sequence of Thauera sp. strain SWB20, isolated from a Singapore wastewater treatment facility using gel microdroplets

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dichosa, Armand E. K.; Davenport, Karen W.; Li, Po-E; Ahmed, Sanaa A.; Daligault, Hajnalka; Gleasner, Cheryl D.; Kunde, Yuliya; McMurry, Kim; Lo, Chien -Chi; Reitenga, Krista G.; et al

    2015-03-19

    In this study, we report here the genome sequence of Thauera sp. strain SWB20, isolated from a Singaporean wastewater treatment facility using gel microdroplets (GMDs) and single-cell genomics (SCG). This approach provided a single clonal microcolony that was sufficient to obtain a 4.9-Mbp genome assembly of an ecologically relevant Thauera species.

  8. Collection efficiency of the soot-particle aerosol mass spectrometer (SP-AMS) for internally mixed particulate black carbon

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Willis, M. D.; Lee, A. K. Y.; Onasch, T. B.; Fortner, E. C.; Williams, L. R.; Lambe, A. T.; Worsnop, D. R.; Abbatt, J. P. D.

    2014-12-18

    The soot-particle aerosol mass spectrometer (SP-AMS) uses an intra-cavity infrared laser to vaporize refractory black carbon (rBC) containing particles, making the particle beam–laser beam overlap critical in determining the collection efficiency (CE) for rBC and associated non-refractory particulate matter (NR-PM). This work evaluates the ability of the SP-AMS to quantify rBC and NR-PM mass in internally mixed particles with different thicknesses of organic coating. Using apparent relative ionization efficiencies for uncoated and thickly coated rBC particles, we report measurements of SP-AMS sensitivity to NR-PM and rBC, for Regal Black, the recommended particulate calibration material. Beam width probe (BWP) measurements aremore » used to illustrate an increase in sensitivity for highly coated particles due to narrowing of the particle beam, which enhances the CE of the SP-AMS by increasing the laser beam–particle beam overlap. Assuming complete overlap for thick coatings, we estimate CE for bare Regal Black particles of 0.6 ± 0.1, which suggests that previously measured SP-AMS sensitivities to Regal Black were underestimated by up to a factor of 2. The efficacy of the BWP measurements is highlighted by studies at a busy road in downtown Toronto and at a non-roadside location, which show particle beam widths similar to, but greater than that of bare Regal Black and coated Regal Black, respectively. Further BWP measurements at field locations will help to constrain the range of CE for fresh and aged rBC-containing particles. The ability of the SP-AMS to quantitatively assess the composition of internally mixed particles is validated through measurements of laboratory-generated organic coated particles, which demonstrate that the SP-AMS can quantify rBC and NR-PM over a wide range of particle compositions and rBC core sizes.« less

  9. Collection efficiency of the Soot-Particle Aerosol Mass Spectrometer (SP-AMS) for internally mixed particulate black carbon

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Willis, M. D.; Lee, A. K. Y.; Onasch, T. B.; Fortner, E. C.; Williams, L. R.; Lambe, A. T.; Worsnop, D. R.; Abbatt, J. P. D.

    2014-05-26

    The soot-particle aerosol mass spectrometer (SP-AMS) uses an intra-cavity infrared laser to vaporize refractory black carbon (rBC) containing particles, making the particle beam–laser beam overlap critical in determining the collection efficiency (CE) for rBC and associated non-refractory particulate matter (NR-PM). This work evaluates the ability of the SP-AMS to quantify rBC and NR-PM mass in internally mixed particles with different thicknesses of organic coating. Using apparent relative ionization efficiencies for uncoated and thickly coated rBC particles, we report measurements of SP-AMS sensitivity to NR-PM and rBC, for Regal Black, the recommended particulate calibration material. Beam width probe (BWP) measurements aremore » used to illustrate an increase in sensitivity for highly coated particles due to narrowing of the particle beam, which enhances the CE of the SP-AMS by increasing the laser beam–particle beam overlap. Assuming complete overlap for thick coatings, we estimate CE for bare Regal Black particles of 0.6 ± 0.1, which suggests that previously measured SP-AMS sensitivities to Regal Black were underestimated by up to a factor of two. The efficacy of the BWP measurements is highlighted by studies at a busy road in downtown Toronto and at a non-roadside location, which show particle beam widths similar to, but greater than that of bare Regal Black and coated Regal Black, respectively. Further BWP measurements at field locations will help to constrain the range of CE for fresh and aged rBC-containing particles. The ability of the SP-AMS to quantitatively assess the composition of internally mixed particles is validated through measurements of laboratory-generated organic coated particles, which demonstrate that the SP-AMS can quantify rBC and NR-PM over a wide range of particle compositions and rBC core sizes.« less

  10. Raman chemical imaging of the rhizosphere bacterium Pantoea sp. YR343 and its co-culture with Arabidopsis thaliana

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Polisetti, Sneha; Bible, Amber N.; Morrell-Falvey, Jennifer L.; Bohn, Paul W.

    2016-02-29

    Chemical imaging of plant-bacteria co-cultures renders it possible to characterize bacterial populations and behaviors and their interactions with proximal organisms, under conditions closest to the environment in the rhizosphere. Here Raman micro-spectroscopy and confocal Raman imaging are used as minimally invasive probes to study the rhizosphere bacterial isolate, Pantoea sp. YR343, and its co-culture with model plant Arabidopsis thaliana by combining enhanced Raman spectroscopies with electron microscopy and principal component analysis (PCA). The presence of carotenoid pigments in the wild type Pantoea sp. YR343 was characterized using resonance Raman scattering, which was also used to confirm successful disruption of themore » crtB gene in an engineered carotenoid mutant strain. Other components of the Pantoea sp. YR343 cells were imaged in the presence of resonantly enhanced pigments using a combination of surface enhanced Raman imaging and PCA. Pantoea sp. YR343 cells decorated with Ag colloid synthesized ex situ gave spectra dominated by carotenoid scattering, whereas colloids synthesized in situ produced spectral signatures characteristic of flavins in the cell membrane. Scanning electron microscopy (SEM) of whole cells and transmission electron microscopy (TEM) images of thinly sliced cross-sections were used to assess structural integrity of the coated cells and to establish the origin of spectral signatures based on the position of Ag nanoparticles in the cells. Finally, raman imaging was also used to characterize senescent green Arabidopsis thaliana plant roots inoculated with Pantoea sp. YR343, and PCA was used to distinguish spectral contributions from plant and bacterial cells, thereby establishing the potential of Raman imaging to visualize the distribution of rhizobacteria on plant roots.« less

  11. Substrate doping: A strategy for enhancing reactivity on gold nanocatalysts by tuning sp bands

    SciTech Connect (OSTI)

    Mammen, Nisha; Narasimhan, Shobhana; Gironcoli, Stefano de

    2015-10-14

    We suggest that the reactivity of Au nanocatalysts can be greatly increased by doping the oxide substrate on which they are placed with an electron donor. To demonstrate this, we perform density functional theory calculations on a model system consisting of a 20-atom gold cluster placed on a MgO substrate doped with Al atoms. We show that not only does such substrate doping switch the morphology of the nanoparticles from the three-dimensional tetrahedral form to the two-dimensional planar form, but it also significantly lowers the barrier for oxygen dissociation by an amount proportional to the dopant concentration. At a doping level of 2.78%, the dissociation barrier is reduced by more than half, which corresponds to a speeding up of the oxygen dissociation rate by five orders of magnitude at room temperature. This arises from a lowering in energy of the s and p states of Au. The d states are also lowered in energy, however, this by itself would have tended to reduce reactivity. We propose that a suitable measure of the reactivity of Au nanoparticles is the difference in energy of sp and d states.

  12. Lunar electric power systems utilizing the SP-100 reactor coupled to dynamic conversion systems. Final report

    SciTech Connect (OSTI)

    Harty, R.B.; Durand, R.E.

    1993-03-01

    An integration study was performed by Rocketdyne under contract to NASA-LeRC. The study was concerned with coupling an SP-0100 reactor to either a Brayton or Stirling power conversion system. The application was for a surface power system to supply power requirements to a lunar base. A power level of 550 kWe was selected based on the NASA Space Exploration Initiative 90-day study. Reliability studies were initially performed to determine optimum power conversion redundancy. This study resulted in selecting three operating engines and one stand-by unit. Integration design studies indicated that either the Brayton or Stirling power conversion systems could be integrated with the PS-100 reactor. The Stirling system had an integration advantage because of smaller piping size and fewer components. The Stirling engine, however, is more complex and heavier than the Brayton rotating unit, which tends to off-set the Stirling integration advantage. From a performance consideration, the Brayton had a 9 percent mass advantage, and the Stirling had a 50 percent radiator advantage.

  13. Isolation, purification and spectrometric analysis of PSP toxins from moraxella sp., a bacterium associated with a toxic dinoflagellate

    SciTech Connect (OSTI)

    Boyce, S.D.; Doucette, G.J.

    1994-12-31

    Paralytic shellfish poisoning (PSP) is a seafood intoxication syndrome caused by the injestion of shellfish contaminated with toxins produced by algae known as dinoflagellates. The PSP toxins, saxitoxin and its derivatives, act to block voltage-dependent sodium channels and can cause paralysis and even death at higher doses. It is well documented that bacteria coexist with many harmful or toxic algal species, though the exact nature of the association in relation to toxin production is unknown. Recently, the bacterium Moraxella sp. was isolated from the PSP toxin producing dinoflagellate Alexandrium tamarense. Through HPLC analysis and saxitoxin receptor binding assays performed on crude bacterial extracts, it appears that Moraxella sp. is capable of producing saxitoxin and several of its derivatives. However, physical confirmation (e.g. mass spectrometry) of these results is still needed.

  14. High-quality permanent draft genome sequence of the Lebeckia ambigua-nodulating Burkholderia sp. strain WSM4176

    SciTech Connect (OSTI)

    De Meyer, Sofie E.; Tian, Rui; Seshadri, Rekha; Reddy, TBK; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Kyrpides, Nikos; Yates, Ron; Howieson, John; Reeve, Wayne

    2015-10-16

    We report that Burkholderia sp. strain WSM4176 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective N2-fixing root nodule of Lebeckia ambigua collected in Nieuwoudtville, Western Cape of South Africa, in October 2007. This plant persists in infertile, acidic and deep sandy soils, and is therefore an ideal candidate for a perennial based agriculture system in Western Australia. Here we describe the features of Burkholderia sp. strain WSM4176, which represents a potential inoculant quality strain for L. ambigua, together with sequence and annotation. The 9,065,247 bp high-quality-draft genome is arranged in 13 scaffolds of 65 contigs, contains 8369 protein-coding genes and 128 RNA-only encoding genes, and is part of the GEBA-RNB project proposal (Project ID 882).

  15. High-quality permanent draft genome sequence of the Lebeckia ambigua-nodulating Burkholderia sp. strain WSM4176

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    De Meyer, Sofie E.; Tian, Rui; Seshadri, Rekha; Reddy, TBK; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Kyrpides, Nikos; Yates, Ron; et al

    2015-10-16

    We report that Burkholderia sp. strain WSM4176 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective N2-fixing root nodule of Lebeckia ambigua collected in Nieuwoudtville, Western Cape of South Africa, in October 2007. This plant persists in infertile, acidic and deep sandy soils, and is therefore an ideal candidate for a perennial based agriculture system in Western Australia. Here we describe the features of Burkholderia sp. strain WSM4176, which represents a potential inoculant quality strain for L. ambigua, together with sequence and annotation. The 9,065,247 bp high-quality-draft genome is arranged in 13 scaffolds of 65 contigs,more » contains 8369 protein-coding genes and 128 RNA-only encoding genes, and is part of the GEBA-RNB project proposal (Project ID 882).« less

  16. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involvedmore » in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.« less

  17. Transcriptional Responses to Sucrose Mimic the Plant-Associated Life Style of the Plant Growth Promoting Endophyte Enterobacter sp. 638

    SciTech Connect (OSTI)

    Taghavi, Safiyh; Wu, Xiao; Ouyang, Liming; Zhang, Yian Biao; Stadler, Andrea; McCorkle, Sean; Zhu, Wei; Maslov, Sergei; van der Lelie, Daniel

    2015-01-21

    Growth in sucrose medium was previously found to trigger the expression of functions involved in the plant associated life style of the endophytic bacterium Enterobacter sp. 638. Therefore, comparative transcriptome analysis between cultures grown in sucrose or lactate medium was used to gain insights in the expression levels of bacterial functions involved in the endophytic life style of strain 638. Growth on sucrose as a carbon source resulted in major changes in cell physiology, including a shift from a planktonic life style to the formation of bacterial aggregates. This shift was accompanied by a decrease in transcription of genes involved in motility (e.g. flagella biosynthesis) and an increase in the transcription of genes involved in colonization, adhesion and biofilm formation. The transcription levels of functions previously suggested as being involved in endophytic behavior and functions responsible for plant growth promoting properties, including the synthesis of indole-acetic acid, acetoin and 2,3-butanediol, also increased significantly for cultures grown in sucrose medium. Interestingly, despite an abundance of essential nutrients transcription levels of functions related to uptake and processing of nitrogen and iron became increased for cultures grown on sucrose as sole carbon source. Transcriptome data were also used to analyze putative regulatory relationships. In addition to the small RNA csrABCD regulon, which seems to play a role in the physiological adaptation and possibly the shift between free-living and plant-associated endophytic life style of Enterobacter sp. 638, our results also pointed to the involvement of rcsAB in controlling responses by Enterobacter sp. 638 to a plant-associated life style. Lastly, targeted mutagenesis was used to confirm this role and showed that compared to wild-type Enterobacter sp. 638 a ΔrcsB mutant was affected in its plant growth promoting ability.

  18. Tectonic denudation of upper mantle along passive margins: a model based on drilling (ODP Leg 103) and diving (Galinaute cruise) results, western Galicia Margin, Spain

    SciTech Connect (OSTI)

    Boillot, G.; Winterer, E.L.; Recq, M.; Girardeau, J.; Kornprobst, J.; Loreau, J.P.; Malod, J.; Mougenot, D.

    1987-05-01

    During ODP Leg 103 (April-June 1985) and the Galinaute cruise (June-July 1986), serpentinized peridotite (clinopyroxene-spinel harzburgite) was recovered within the basement approximately at the boundary between the North Atlantic ocean crust to the west and the thinned continental crust of the Galicia passive margin (Spain) to the east. The exposure of mantle-derived peridotite on the sea floor occurred at the end of the period of rifting, roughly 110 Ma. Ductile shear zones observed in the peridotite are consistent with movements along a deep, low-angle normal fault rooted within the upper mantle and dipping eastward beneath the Galicia margin. To explain the tectonic denudation of the mantle at the ocean-continent boundary, they use a nonuniform stretching model for the lithosphere, set up from Wernicke's model.

  19. Overview of the 1986 free-piston Stirling SP-100 activities at the NASA Lewis Research Center

    SciTech Connect (OSTI)

    Slaby, J.G.

    1986-01-01

    An overview of the National Aeronautics and Space Administration (NASA) Lewis Research Center SP-100 free-piston Stirling engine activities is presented. These activities include a free-piston Stirling space-power technology feasibility demonstration project as part of the SP-100 program being conducted in support of the Department of Defense (DOD), Department of Energy (DOE), and NASA. The space-power Stirling advanced technology effort, under SP-100, addresses the status of the 25 kWe Space Power Demonstrator Engine (SPDE) including test results. Future space-power projections are presented along with a description of a study that will investigate the feasibility of scaling a single-cylinder free-piston Stirling space-power module to the 150 kW power range. Design parameters and conceptual design features will be presented for a 25 kWe, single-cylinder free-piston Stirling space-power converter. A description of a hydrodynamic gas bearing concept will be presented whereby the displacer of a 1 kWe free-piston Stirling engine is modified to demonstrate the bearing concept. And finally the goals of a conceptual design for a 25 kWe Solar Advanced Stirling Conversion System capable of delivering electric power to an electric utility grid will be discussed. The solar work is under an interagency agreement between DOE/Sandia National Laboratory and NASA Lewis.

  20. Elastic properties, sp³ fraction, and Raman scattering in low and high pressure synthesized diamond-like boron rich carbides

    SciTech Connect (OSTI)

    Zinin, Pavel V.; Burgess, Katherine; Jia, Ruth; Sharma, Shiv; Ming, Li-Chung; Liu, Yongsheng; Ciston, Jim; Hong, Shiming

    2014-10-07

    Dense BC{sub x} phases with high boron concentration are predicted to be metastable, superhard, and conductors or superconductors depending on boron concentration. However, up to this point, diamond-like boron rich carbides BC{sub x} (dl-BC{sub x}) phases have been thought obtainable only through high pressure and high temperature treatment, necessitating small specimen volume. Here, we use electron energy loss spectroscopy combined with transmission electron microscopy, Raman spectroscopy, surface Brillouin scattering, laser ultrasonics (LU) technique, and analysis of elastic properties to demonstrate that low pressure synthesis (chemical vapor deposition) of BC{sub x} phases may also lead to the creation of diamond-like boron rich carbides. The elastic properties of the dl-BC{sub x} phases depend on the carbon sp²versus sp³ content, which decreases with increasing boron concentration, while the boron bonds determine the shape of the Raman spectra of the dl-BC{sub x} after high pressure-high temperature treatment. Using the estimation of the density value based on the sp³ fraction, the shear modulus μ of dl-BC₄, containing 10% carbon atoms with sp³ bonds, and dl-B₃C₂, containing 38% carbon atoms with sp³ bonds, were found to be μ = 19.3 GPa and μ = 170 GPa, respectively. The presented experimental data also imply that boron atoms lead to a creation of sp³ bonds during the deposition processes.

  1. Description of Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer

    SciTech Connect (OSTI)

    Prakash, Om; Green, Stefan; Jasrotia, Puja; Overholt, Will; Canion, Andy; Watson, David B; Brooks, Scott C; Kostka,

    2012-01-01

    Bacterial strains 2APBS1T and 116-2 were isolated from the subsurface of a nuclear legacy waste site where sediments are co-contaminated with large amounts of acidity, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains represent the first members of the Rhodanobacter genus shown to be capable of complete denitrification. Cells of strain 2APBS1T and 116-2 were Gram negative, non-spore-forming, rods, 3-5 micro;m long and 0.25-0.5 m in diameter. The isolates were facultative anaerobes, and had temperature and pH optima for growth at 30 C and pH 6.5, respectively, and could tolerate up to 2.0 % NaCl, though growth improved in its absence. Strains 2APBS1T and 116-2 contained fatty acid profiles and 100 % Q-8 ubiquinone, that are characteristic features of the genus Rhodanobacter. Although strains 2APBS1T and 116-2 share high SSU rRNA gene sequence similarity to R. thiooxydans (>99%), DNA-DNA hybridization values were substantially below the 70% threshold used to designate novel species. Thus, based on genotypic, phylogenetic, chemotaxonomic and physiological differences, strains 2APBS1T and 116-2 are considered to represent a novel species of the genus Rhodanobacter, for which the name Rhodanobacter denitrificans sp. nov is proposed. The type strain is 2APBS1T (=DSM 23569T =JCM 17641T). Strain 116-2 (=DSM 24678 = JCM 17642) is a reference strain.

  2. Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2

    SciTech Connect (OSTI)

    Blum, D.L.; Li, X.L.; Chen, H.; Ljungdahl, L.G.

    1999-09-01

    A 1,067-bp cDNA, designated axeA, coding for an acetyl xylan esterase (AxeA) was cloned from the anaerobic rumen fungus Orpinomyces sp. strain PC-2. The gene had an open reading frame of 939 bp encoding a polypeptide of 313 amino acid residues with a calculated mass of 34,845 Da. An active esterase using the original start codon of the cDNA was synthesized in Escherichia coli. Two active forms of the esterase were purified from recombinant E. coli cultures. The size difference of 8 amino acids was a result of cleavages at two different sites within the signal peptide. The enzyme released acetate from several acetylated substrates, including acetylated xylan. The activity toward acetylated xylan was tripled in the presence of recombinant xylanase A from the same fungus. Using p-nitrophenyl acetate as a substrate, the enzyme had a K{sub m} of 0.9 mM and a V{sub max} of 785 {micro}mol min{sup {minus}} mg{sup {minus}1}. It had temperature and pH optima of 30 C and 9.0, respectively. AxeA had 56% amino acid identity with BnaA, an acetyl xylan esterase of Neocallimastix patriciarum, but the Orpinomyces AxeA was devoid of a noncatalytic repeated peptide domain (NCRPD) found at the carboxy terminus of the Neocallimastix BnaA. The NCRPD found in many glycosyl hydrolases and esterases of anaerobic fungi has been postulated to function as a docking domain for cellulase-hemicellulase complexes, similar to the dockerin of the cellulosome of Clostridium thermocellum.

  3. SpArcFiRe: Scalable automated detection of spiral galaxy arm segments

    SciTech Connect (OSTI)

    Davis, Darren R.; Hayes, Wayne B. E-mail: whayes@uci.edu

    2014-08-01

    Given an approximately centered image of a spiral galaxy, we describe an entirely automated method that finds, centers, and sizes the galaxy (possibly masking nearby stars and other objects if necessary in order to isolate the galaxy itself) and then automatically extracts structural information about the spiral arms. For each arm segment found, we list the pixels in that segment, allowing image analysis on a per-arm-segment basis. We also perform a least-squares fit of a logarithmic spiral arc to the pixels in that segment, giving per-arc parameters, such as the pitch angle, arm segment length, location, etc. The algorithm takes about one minute per galaxies, and can easily be scaled using parallelism. We have run it on all ?644,000 Sloan objects that are larger than 40 pixels across and classified as 'galaxies'. We find a very good correlation between our quantitative description of a spiral structure and the qualitative description provided by Galaxy Zoo humans. Our objective, quantitative measures of structure demonstrate the difficulty in defining exactly what constitutes a spiral 'arm', leading us to prefer the term 'arm segment'. We find that pitch angle often varies significantly segment-to-segment in a single spiral galaxy, making it difficult to define the pitch angle for a single galaxy. We demonstrate how our new database of arm segments can be queried to find galaxies satisfying specific quantitative visual criteria. For example, even though our code does not explicitly find rings, a good surrogate is to look for galaxies having one long, low-pitch-angle armwhich is how our code views ring galaxies. SpArcFiRe is available at http://sparcfire.ics.uci.edu.

  4. Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002

    SciTech Connect (OSTI)

    Bernstein, Hans C.; Konopka, Allan; Melnicki, Matthew R.; Hill, Eric A.; Kucek, Leo A.; Zhang, Shuyi; Shen, Gaozhong; Bryant, Donald A.; Beliaev, Alex S.

    2014-09-19

    Synechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality was systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily by chlorophyll (680 nm) or phycocyanin (630 nm) as the organism was subjected to four distinct mono- and dichromatic regimes. During cultivation with dichromatic light, growth rates displayed by Synechococcus sp. PCC 7002 were generally proportional to the total incident irradiance at values < 275 mol photons m-2 s-1 and were not affected by the ratio of 630:680 nm wavelengths. Notably, under monochromatic light conditions, cultures exhibited similar growth rates only when they were irradiated with 630 nm light; cultures irradiated with only 680 nm light grew at rates that were 60 70% of those under other light quality regimes at equivalent irradiances. The functionality of photosystem II and associated processes such as maximum rate of photosynthetic electron transport, rate of cyclic electron flow, and rate of dark respiration generally increased as a function of growth rate. Nonetheless, some of the photophysiological parameters measured here displayed distinct patterns with respect to growth rate of cultures adapted to a single wavelength including phycobiliprotein content, which increased under severely light-limited growth conditions. Additionally, the ratio of photosystem II to photosystem I increased approximately 40% over the range of growth rates, although cells grown with 680 nm light only had the highest ratios. These results suggest the presence of effective mechanisms which allow acclimation of Synechococcus sp. PCC 7002 acclimation to different irradiance conditions.

  5. Reversible elastic deformation of functionalized sp{sup 2} carbon at pressures of up to 33?GPa

    SciTech Connect (OSTI)

    Soignard, Emmanuel; Hochheimer, Hans D.; Yarger, Jeff; Raj, Rishi

    2014-10-06

    We show that sp{sup 2} carbon bonded to silicon and oxygen can withstand reversible elastic deformation at pressures of up to 33?GPa. These experiments were carried out in a diamond anvil cell. In-situ Raman spectroscopy was employed to record the reversibility of elastic deformation by measuring the movement in the D and G peaks of carbon. Above 33?GPa the material, a silicon oxycarbide, transforms into an unidentified state which is retained upon unloading down to ambient pressure. Thermodynamical analysis suggests that the material could have transformed into a crystalline state at these ultrahigh pressures, driven by mechanical work.

  6. Evidence regarding the UV sunscreen role of a mycosporine-like compound in the cyanobacterium Gloeocapsa sp

    SciTech Connect (OSTI)

    Garcia-Pichel, F.; Wingard, C.E.; Castenholz, R.W. )

    1993-01-01

    The mycosporine-like amino acids (MAAs) have been thought to serve a UV sunscreen role in organisms that produce or contain them because MAAs present strong absorbance in the UV region and because there is no other apparent biological function. The researchers used the cyanobacterium Gloeocapsa sp. to assess the possible sunscreen role of MAAs. Five conditions are evaluated: (1) absorption of radiation high enough to provide benefit to the organisms; (2) correlation of presence of the compound with enhansed fitness under UV; (3) concentration of the compound and resistance to UV still present under physiological inactivity; (4) effect maximal at wavelengths of maximal absorption; (5) loss of protection after artificial removal of compound. The results indicate that only a small sunscreen effect can be ascribed to the MAA in the Gloecapsa sp. under these experimental conditions. It is possible however, that in the typical undisturbed colonial growth form, MAAs and their screening action may become major factors in resistance to UV radiation. 25 refs., 7 figs., 1 tab.

  7. High-quality permanent draft genome sequence of the Mimosa asperata - nodulating Cupriavidus sp. strain AMP6

    SciTech Connect (OSTI)

    De Meyer, Sofie E.; Parker, Matthew; Van Berkum, Peter; Tian, Rui; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Kyrpides, Nikos; Howieson, John; Reeve, Wayne

    2015-10-16

    Cupriavidus sp. strain AMP6 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Mimosa asperata collected in Santa Ana National Wildlife Refuge, Texas, in 2005. Mimosa asperata is the only legume described so far to exclusively associates with Cupriavidus symbionts. Furthermore, strain AMP6 represents an early-diverging lineage within the symbiotic Cupriavidus group and has the capacity to develop an effective nitrogen-fixing symbiosis with three other species of Mimosa. Here, we describe the genome of Cupriavidus sp. strain AMP6 which enables comparative analyses of symbiotic trait evolution in this genus; the general features, together with sequence and annotation are further discussed. Finally, the 7,579,563 bp high-quality permanent draft genome is arranged in 260 scaffolds of 262 contigs, contains 7,033 protein-coding genes and 97 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.

  8. Expression, purification and preliminary X-ray characterization of dl-2-haloacid dehalogenase from Methylobacterium sp. CPA1

    SciTech Connect (OSTI)

    Omi, Rie; Jitsumori, Keiji; Yamauchi, Takahiro; Ichiyama, Susumu; Kurihara, Tatsuo; Esaki, Nobuyoshi; Kamiya, Nobuo; Hirotsu, Ken Miyahara, Ikuko

    2007-07-01

    A recombinant form of dl-2-haloacid dehalogenase from Methylobacterium sp. CPA1 has been expressed in E. coli, purified and crystallized. The crystal belongs to space group P6{sub 3}. Diffraction data have been collected to 1.75 Å resolution. dl-2-Haloacid dehalogenase from Methylobacterium sp. CPA1 (dl-DEX Mb) is a unique enzyme that catalyzes the dehalogenation reaction without the formation of an ester intermediate. A recombinant form of dl-DEX Mb has been expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal belongs to the hexagonal space group P6{sub 3}, with unit-cell parameters a = b = 186.2, c = 114.4 Å. The crystals are likely to contain between four and eight monomers in the asymmetric unit, with a V{sub M} value of 4.20–2.10 Å{sup 3} Da{sup −1}. A self-rotation function revealed peaks on the χ = 180° section. X-ray data have been collected to 1.75 Å resolution.

  9. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  10. Nucleotide sequence of a chromosomal mercury resistance determinant from a Bacillus sp. with broad-spectrum mercury resistance. [Mercury reductase

    SciTech Connect (OSTI)

    Wang, Y.; Levinson, H.S.; Mahler, I. ); Moore, M.; Walsh, C. ); Silver, S. )

    1989-01-01

    A 13.5-kilobase HindIII fragment, bearing an intact mercury resistance (mer) operon, was isolated from chromosomal DNA of broad-spectrum mercury-resistant Bacillus sp. strain RC607 by using as a probe a clone containing the mercury reductase (merA) gene. The new clone, pYW33, expressed broad-spectrum mercury resistance both in Escherichia coli and in Bacillus subtilis, but only in B. subtilis was the mercuric reductase activity inducible. Sequencing of a 1.8-kilobase mercury hypersensitivity-producing fragment revealed four open reading frames (ORFs). ORF1 may code for a regulatory protein (MerR). ORF2 and ORF4 were associated with cellular transport function and the hypersensitivity phenotype. DNA fragments encompassing the merA and the merB genes were sequenced. The predicted Bacillus sp. strain RC607 MerA (mercuric reductase) and MerB (organomercurial lyase) were similar to those predicted from Staphylococcus aureus plasmid pI258 (67 and 73% amino acid identities, respectively); however, only 40% of the amino acid residues of RC607 MerA were identical to those of the mercuric reductase from gram-negative bacteria. A 69-kilodalton polypeptide was isolated and identified as the merA gene product by examination of its amino-terminal sequence.

  11. High-quality permanent draft genome sequence of the Mimosa asperata - nodulating Cupriavidus sp. strain AMP6

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    De Meyer, Sofie E.; Parker, Matthew; Van Berkum, Peter; Tian, Rui; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; et al

    2015-10-16

    Cupriavidus sp. strain AMP6 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Mimosa asperata collected in Santa Ana National Wildlife Refuge, Texas, in 2005. Mimosa asperata is the only legume described so far to exclusively associates with Cupriavidus symbionts. Furthermore, strain AMP6 represents an early-diverging lineage within the symbiotic Cupriavidus group and has the capacity to develop an effective nitrogen-fixing symbiosis with three other species of Mimosa. Here, we describe the genome of Cupriavidus sp. strain AMP6 which enables comparative analyses of symbiotic trait evolution in this genus; the general features, together withmore » sequence and annotation are further discussed. Finally, the 7,579,563 bp high-quality permanent draft genome is arranged in 260 scaffolds of 262 contigs, contains 7,033 protein-coding genes and 97 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.« less

  12. High-quality permanent draft genome sequence of the Parapiptadenia rigida-nodulating Cupriavidus sp. strain UYPR2.512

    SciTech Connect (OSTI)

    De Meyer, Sofie E.; Fabiano, Elena; Tian, Rui; Van Berkum, Peter; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia N.; Pati, Amrita; Woyke, Tanja; Howieson, John; Kyrpides, Nikos C.; Reeve, Wayne

    2015-04-11

    Cupriavidus sp. strain UYPR2.512 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Parapiptadenia rigida grown in soils from a native forest of Uruguay. Here we describe the features of Cupriavidus sp. strain UYPR2.512, together with sequence and annotation. We find the 7,858,949 bp high-quality permanent draft genome is arranged in 365 scaffolds of 369 contigs, contains 7,411 protein-coding genes and 76 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.

  13. Genome Sequence of Thermotoga sp Strain RQ2, a Hyperthermophilic Bacterium Isolated from a Geothermally Heated Region of the Seafloor near Ribeira Quente, the Azores

    SciTech Connect (OSTI)

    Swithers, Kristen S; DiPippo, Jonathan L; Bruce, David; Detter, J. Chris; Tapia, Roxanne; Han, Cliff; Woyke, Tanja; Pitluck, Sam; Pennacchio, Len; Nolan, Matt; Mikhailova, Natalia; Lykidis, A; Land, Miriam L; Stetter, Karl O; Nelson, Karen E; Gogarten, Peter; Noll, Kenneth M

    2011-01-01

    Thermotoga sp. strain RQ2 is probably a strain of Thermotoga maritima. Its complete genome sequence allows for an examination of the extent and consequences of gene flow within Thermotoga species and strains. Thermotoga sp. RQ2 differs from T. maritima in its genes involved in myo-inositol metabolism. Its genome also encodes an apparent fructose phosphotransferase system (PTS) sugar transporter. This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Thermotogales. These are the first reported PTS transporters in the Thermotogales.

  14. Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos

    SciTech Connect (OSTI)

    Tiwari, Ravi; Howieson, John; Yates, Ron; Tian, Rui; Held, Britanny; Tapia, Roxanne; Han, Cliff; Seshadri, Rekha; Reddy, T. B. K.; Huntemann, Marcel; Pati, Amrita; Woyke, Tanja; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Reeve, Wayne

    2015-11-30

    Bradyrhizobium sp. WSM1253 is a novel N2-fixing bacterium isolated from a root nodule of the herbaceous annual legume Ornithopus compressus that was growing on the Greek Island of Sifnos. WSM1253 emerged as a strain of interest in an Australian program that was selecting inoculant quality bradyrhizobial strains for inoculation of Mediterranean species of lupins ( Lupinus angustifolius, L. princei, L. atlanticus, L. pilosus ). In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 8,719,808 bp genome has a G + C content of 63.09 % with 71 contigs arranged into two scaffolds. The assembled genome contains 8,432 protein-coding genes, 66 RNA genes and a single rRNA operon. In conclusion, this improved-high-quality draft rhizobial genome is one of 20 sequenced through a DOE Joint Genome Institute 2010 Community Sequencing Project.

  15. Genome sequence of Bradyrhizobium sp. WSM1253; a microsymbiont of Ornithopus compressus from the Greek Island of Sifnos

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tiwari, Ravi; Howieson, John; Yates, Ron; Tian, Rui; Held, Britanny; Tapia, Roxanne; Han, Cliff; Seshadri, Rekha; Reddy, T. B. K.; Huntemann, Marcel; et al

    2015-11-30

    Bradyrhizobium sp. WSM1253 is a novel N2-fixing bacterium isolated from a root nodule of the herbaceous annual legume Ornithopus compressus that was growing on the Greek Island of Sifnos. WSM1253 emerged as a strain of interest in an Australian program that was selecting inoculant quality bradyrhizobial strains for inoculation of Mediterranean species of lupins ( Lupinus angustifolius, L. princei, L. atlanticus, L. pilosus ). In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 8,719,808 bp genome has a G + C content of 63.09 % with 71 contigs arrangedmore » into two scaffolds. The assembled genome contains 8,432 protein-coding genes, 66 RNA genes and a single rRNA operon. In conclusion, this improved-high-quality draft rhizobial genome is one of 20 sequenced through a DOE Joint Genome Institute 2010 Community Sequencing Project.« less

  16. Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bernstein, Hans C.; Charania, Moiz A.; McClure, Ryan S.; Sadler, Natalie C.; Melnicki, Matthew R.; Hill, Eric A.; Markillie, Lye Meng; Nicora, Carrie D.; Wright, Aaron T.; Romine, Margaret F.; et al

    2015-11-03

    This study combines transcriptomic and proteomic profiling to provide new insights on the metabolic relationship between oxygenic photosynthesis and nitrogenase-mediated H2 production in the model cyanobacterium, Cyanothece sp. ATCC 51142. To date, the proposed mechanisms used to describe the energy metabolism processes that support H2 production in Cyanothece 51142 have assumed that ATP and reductant requirements are derived solely from glycogen oxidation and/or cyclic-electron flow around photosystem I. The results from this study present and test an alternative hypothesis by showing that net-positive rates of oxygenic photosynthesis and increased expression of photosystem II reaction centers correspond and are synchronized withmore » nitrogenase expression and H2 production. These findings provide a new and more complete view on the metabolic processes contributing to the energy budget of photosynthetic H2 production and highlight the likely role of photocatalytic H2O oxidation as a major participating process.« less

  17. Mutations that affect structure and assembly of light-harvesting proteins in the cyanobacterium Synechocystis sp. strain 6701

    SciTech Connect (OSTI)

    Anderson, L.K.; Rayner, M.C.; Eiserling, F.A.

    1987-01-01

    The unicellular cyanobacterium Synechocystis sp. strain 6701 was mutagenized with UV irradiation and screened for pigment changes that indicated genetic lesions involving the light-harvesting proteins of the phycobilisome. A previous examination of the pigment mutant UV16 showed an assembly defect in the phycocyanin component of the phycobilisome. Mutagenesis of UV16 produced an additional double mutant, UV16-40, with decreased phycoerythrin content. Phycocyanin and phycoerythrin were isolated from UV16-40 and compared with normal biliproteins. The results suggested that the UV16 mutation affected the alpha subunit of phycocyanin, while the phycoerythrin beta subunit from UV16-40 had lost one of its three chromophores. Characterization of the unassembled phycobilisome components in these mutants suggests that these strains will be useful for probing in vivo the regulated expression and assembly of phycobilisomes.

  18. Draft Genome sequence of Frankia sp. Strain QA3, a nitrogen-fixing actinobacterium isolated from the root nodule of Alnus nitida

    SciTech Connect (OSTI)

    Sen, Arnab; Beauchemin, Nicholas; Bruce, David; Chain, Patrick S. G.; Chen, Amy; Davenport, Karen W.; Deshpande, Shweta; Detter, J. Chris; Furnholm, Teal; Ghodhbane-Gtari, Faten; Goodwin, Lynne A.; Gtari, Maher; Han, James; Huntemann, Marcel; Ivanova, N; Kyrpides, Nikos C; Land, Miriam L; Markowitz, Victor; Mavromatis, K; Nolan, Matt; Nouioui, Imen; Pagani, Ioanna; Pati, Amrita; Pitluck, Sam; Santos, Catarina; Sur, Saubashya; Szeto, Ernest; Tavares, Fernando; Teshima, Hazuki; Thakur, Subarna; Wall, Luis; Woyke, Tanja; Wishart, Jessie; Tisa, Louis S.

    2013-01-01

    Members of actinomycete genus Frankia form a nitrogen-fixing symbiosis with 8 different families of actinorhizal plants. We report a high-quality draft genome sequence for Frankia sp. stain QA3, a nitrogen-fixing actinobacterium isolated from root nodules of Alnus nitida.

  19. Draft Genome sequence of Frankia sp. strains CN3 , an atypical, non-infective (Nod-) ineffective (Fix-) isolate from Coriaria nepalensis

    SciTech Connect (OSTI)

    Ghodhbane-Gtari, Faten; Beauchemin, Nicholas; Bruce, David; Chain, Patrick S. G.; Chen, Amy; Davenport, Karen W.; Deshpande, Shweta; Detter, J. Chris; Furnholm, Teal; Goodwin, Lynne A.; Gtari, Maher; Han, Cliff; Han, James; Huntemann, Marcel; Ivanova, N; Kyrpides, Nikos C; Land, Miriam L; Markowitz, Victor; Mavromatis, K; Nolan, Matt; Nouioui, Imen; Pagani, Ioanna; Pati, Amrita; Pitluck, Sam; Santos, Catarina; Sen, Arnab; Sur, Saubashya; Szeto, Ernest; Tavares, Fernando; Hazuki, Teshima; Thakur, Subarna; Wall, Luis; Woyke, Tanja; Tisa, Louis S.

    2013-01-01

    We report here the genome sequence of Frankia sp. strain CN3, which was isolated from Coriaria nepalensis. This genome sequence is the first from the fourth lineage of Frankia, that are unable to re-infect actinorhizal plants. At 10 Mb, it represents the largest Frankia genome sequenced to date.

  20. The impact of carbon sp{sup 2} fraction of reduced graphene oxide on the performance of reduced graphene oxide contacted organic transistors

    SciTech Connect (OSTI)

    Kang, Narae; Khondaker, Saiful I.

    2014-12-01

    One of the major bottlenecks in fabricating high performance organic field effect transistors (OFETs) is a large interfacial contact barrier between metal electrodes and organic semiconductors (OSCs) which makes the charge injection inefficient. Recently, reduced graphene oxide (RGO) has been suggested as an alternative electrode material for OFETs. RGO has tunable electronic properties and its conductivity can be varied by several orders of magnitude by varying the carbon sp{sup 2} fraction. However, whether the sp{sup 2} fraction of RGO in the electrode affects the performance of the fabricated OFETs is yet to be investigated. In this study, we demonstrate that the performance of OFETs with pentacene as OSC and RGO as electrode can be continuously improved by increasing the carbon sp{sup 2} fraction of RGO. When compared to control palladium electrodes, the mobility of the OFETs shows an improvement of ∼200% for 61% sp{sup 2} fraction RGO, which further improves to ∼500% for 80% RGO electrode. Similar improvements were also observed in current on-off ratio, on-current, and transconductance. Our study suggests that, in addition to π-π interaction at RGO/pentacene interface, the tunable electronic properties of RGO electrode have a significant role in OFETs performance.

  1. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.; Bryant, Donald A.; Peters, John W.

    2014-10-18

    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.

  2. Complete genome sequence of Anaeromyxobacter sp. Fw109-5, an Anaerobic, Metal-Reducing Bacterium Isolated from a Contaminated Subsurface Environment

    SciTech Connect (OSTI)

    Hwang, C.; Copeland, A.; Lucas, Susan; Lapidus, Alla; Barry, Kerrie W.; Glavina del Rio, T.; Dalin, Eileen; Tice, Hope; Pitluck, S.; Sims, David R.; Brettin, T.; Bruce, David; Detter, J. C.; Han, Cliff F.; Schmutz, Jeremy; Larimer, F.; Land, M.; Hauser, L.; Kyrpides, Nikos C.; Lykidis, Athanasios; Richardson, P. M.; Beliaev, Alex S.; Sanford, Robert A.; Loeffler, Frank E.; Fields, Matthew W.

    2015-01-22

    We report the genome sequence of Anaeromyxobacter sp. Fw109-5, isolated from nitrate- and uranium-contaminated subsurface sediment of the Oak Ridge Integrated Field-Scale Subsurface Research Challenge (IFC) site, Oak Ridge Reservation, TN. The bacteriums genome sequence will elucidate its physiological potential in subsurface sediments undergoing in situ uranium bioremediation and natural attenuation.

  3. Draft genome sequence of Streptomyces sp. strain Wb2n-11, a desert isolate with broad-spectrum antagonism against soilborne phytopathogens

    SciTech Connect (OSTI)

    Koeberl, Martina; White, Richard A.; Erschen, Sabine; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-08-06

    Streptomyces sp. strain Wb2n-11, isolated from native desert soil, exhibited broad-spectrum antagonism against plant pathogenic fungi, bacteria and nematodes. The 8.2 Mb draft genome reveals genes putatively responsible for its promising biocontrol activity and genes which enable the soil bacterium to directly interact beneficially with plants.

  4. Genome Sequence of Halomonas sp. Strain KO116, an Ionic Liquid- Tolerant Marine Bacterium Isolated from a Lignin-Enriched Seawater Microcosm

    SciTech Connect (OSTI)

    O'Dell, Kaela; Woo, Hannah L.; Utturkar, Sagar M.; Klingeman, Dawn Marie; Brown, Steven D.; Hazen, Terry C.

    2015-05-07

    Halomonas sp. strain KO116 was isolated from Nile Delta Mediterranean Sea surface water enriched with insoluble organosolv lignin. It was further screened for growth on alkali lignin minimal salts medium agar. The strain tolerates the ionic liquid 1-ethyl-3-methylimidazolium acetate. Its complete genome sequence is presented in this report.

  5. Complete genome sequence of Anaeromyxobacter sp. Fw109-5, an Anaerobic, Metal-Reducing Bacterium Isolated from a Contaminated Subsurface Environment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hwang, C.; Copeland, A.; Lucas, Susan; Lapidus, Alla; Barry, Kerrie W.; Glavina del Rio, T.; Dalin, Eileen; Tice, Hope; Pitluck, S.; Sims, David R.; et al

    2015-01-22

    We report the genome sequence of Anaeromyxobacter sp. Fw109-5, isolated from nitrate- and uranium-contaminated subsurface sediment of the Oak Ridge Integrated Field-Scale Subsurface Research Challenge (IFC) site, Oak Ridge Reservation, TN. The bacterium’s genome sequence will elucidate its physiological potential in subsurface sediments undergoing in situ uranium bioremediation and natural attenuation.

  6. Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Brumm, Phillip; Land, Miriam L.; Hauser, Loren J.; Jeffries, Cynthia D.; Chang, Yun-Juan; Mead, David A.

    2015-10-19

    Geobacillus sp. Y412MC52 was isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). Based on 16S rRNA genes and average nucleotide identity, Geobacillus sp. Y412MC52 and the related Geobacillus sp. Y412MC61 appear to be members of a new species of Geobacillus. The genome of Geobacillus sp. Y412MC52 consists of one circular chromosome of 3,628,883 bp, an average G+C content of 52 % and one circular plasmid of 45,057 bp andmore » an average G+C content of 45 %. Y412MC52 possesses arabinan, arabinoglucuronoxylan, and aromatic acid degradation clusters for degradation of hemicellulose from biomass. Lastly, transport and utilization clusters are also present for other carbohydrates including starch, cellobiose, and α- and β-galactooligosaccharides.« less

  7. A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bible, Amber; Fletcher, Sarah J; Pelletier, Dale A; Schadt, Christopher Warren; Jawdy, Sara; Weston, David; Engle, Nancy L.; Tschaplinski, Timothy J.; Masyuko, Rachel; Polisetti, Sneha; et al

    2016-04-18

    The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically-important plants. Pantoea sp. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid. Pantoea sp. YR343 readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. Tomore » better understand the molecular mechanisms involved in plant association and rhizosphere survival by Pantoea sp. YR343, we constructed a mutant in which the crtB gene encoding phytoene synthase was deleted. Phytoene synthase is responsible for converting geranylgeranyl pyrophosphate to phytoene, an important precursor to the production of carotenoids. As predicted, the ΔcrtB mutant is defective in carotenoid production, and shows increased sensitivity to oxidative stress. Moreover, we find that the ΔcrtB mutant is impaired in biofilm formation and production of indole-3-acetic acid. Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots. Taken together, these data suggest that carotenoids are important for plant association and/or rhizosphere survival in Pantoea sp. YR343.« less

  8. Draft Genome Sequence of Streptomyces sp. Strain Wb2n-11, a Desert Isolate with Broad-Spectrum Antagonism against Soilborne Phytopathogens

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Köberl, Martina; White, Richard A.; Erschen, Sabine; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-08-06

    Streptomyces sp. strain Wb2n-11, isolated from native desert soil, exhibited broad-spectrum antagonism against plant pathogenic fungi, bacteria, and nematodes. The 8.2-Mb draft genome reveals genes putatively responsible for its promising biocontrol activity and genes which enable the soil bacterium to directly interact beneficially with plants.

  9. Draft Genome Sequence of Streptomyces sp. Strain Wb2n-11, a Desert Isolate with Broad-Spectrum Antagonism against Soilborne Phytopathogens

    SciTech Connect (OSTI)

    Köberl, Martina; White, Richard A.; Erschen, Sabine; El-Arabi, Tarek F.; Jansson, Janet K.; Berg, Gabriele

    2015-08-06

    Streptomyces sp. strain Wb2n-11, isolated from native desert soil, exhibited broad-spectrum antagonism against plant pathogenic fungi, bacteria, and nematodes. The 8.2-Mb draft genome reveals genes putatively responsible for its promising biocontrol activity and genes which enable the soil bacterium to directly interact beneficially with plants.

  10. Complete genome sequence of Anaeromyxobacter sp. Fw109-5, an anaerobic, metal-reducing bacterium isolated from a contaminated subsurface environment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hwang, C.; Copeland, A.; Lucas, S.; Lapidus, A.; Barry, K.; Glavina del Rio, T.; Dalin, E.; Tice, H.; Pitluck, S.; Sims, D.; et al

    2015-01-22

    We report the genome sequence of Anaeromyxobacter sp. Fw109-5, isolated from nitrate- and uranium-contaminated subsurface sediment of the Oak Ridge Integrated Field-Scale Subsurface Research Challenge (IFC) site, Oak Ridge Reservation, TN. The bacteriums genome sequence will elucidate its physiological potential in subsurface sediments undergoing in situ uranium bioremediation and natural attenuation.

  11. Draft genome sequence of Thauera sp. strain SWB20, isolated from a Singapore wastewater treatment facility using gel microdroplets

    SciTech Connect (OSTI)

    Dichosa, Armand E. K.; Davenport, Karen W.; Li, Po-E; Ahmed, Sanaa A.; Daligault, Hajnalka; Gleasner, Cheryl D.; Kunde, Yuliya; McMurry, Kim; Lo, Chien -Chi; Reitenga, Krista G.; Daughton, Ashlynn R.; Shen, Xiaohong; Frietze, Seth; Wang, Dongping; Johnson, Shannon L.; Drautz-Moses, Daniela I.; Schuster, Stephan; Chain, Patrick S.; Han, Cliff

    2015-03-19

    In this study, we report here the genome sequence of Thauera sp. strain SWB20, isolated from a Singaporean wastewater treatment facility using gel microdroplets (GMDs) and single-cell genomics (SCG). This approach provided a single clonal microcolony that was sufficient to obtain a 4.9-Mbp genome assembly of an ecologically relevant Thauera species.

  12. Office of Communication - Brochures Available

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tadjikistan Taiwan Tanzania Thailand Togo Tokelau Tonga Trinidad and Tobago Tunisia Turkey Turkmenistan Turks & Caicos Islands Tuvalu Uganda Ukraine United Arab Emirates United...

  13. Oil country tubular goods from Argentina, Austria, Italy, Japan, Korea, Mexico, and Spain. Investigations Nos. 701-ta-363 and 364 (final) and investigations nos. 731-ta-711-717 (final)

    SciTech Connect (OSTI)

    1995-08-01

    Based on the record in these final investigations, we determine that an industry in the United States is materially injured by reason of imports of oil country tubular goods excluding drill pipe (also referred to herein as `casing and tubing`) from Argentina, Italy, Japan, Korea, and Mexico that are sold in the United States is materially injured by (`LTFV`). We also determine that an industry in the United States is materially injured by reason of subsidized imports of casing and tubing from Italy. We find that an industry in the United States is not materially injured or threatened with material injury by reason of imports of casing and tubing from Austria and Spain that are sold at LTFV or subsidized. We further determine that an industry in the United States is threatened with material injury by reason of imports of drill pipe from Argentina, Japan, and Mexico that are sold at LTFV. We determine that we would not have made an affirmative material injury determination but for the suspension of liquidation. Finally, we unanimously determine that an industry in the United States is neither materially injured nor threatened with material injury by reason of LFTV or subsidized imports of drill pipe from Austria, Italy, Korea, or Spain.

  14. Hybrid radiosity-SP{sub 3} equation based bioluminescence tomography reconstruction for turbid medium with low- and non-scattering regions

    SciTech Connect (OSTI)

    Chen, Xueli E-mail: jimleung@mail.xidian.edu.cn; Zhang, Qitan; Yang, Defu; Liang, Jimin E-mail: jimleung@mail.xidian.edu.cn

    2014-01-14

    To provide an ideal solution for a specific problem of gastric cancer detection in which low-scattering regions simultaneously existed with both the non- and high-scattering regions, a novel hybrid radiosity-SP{sub 3} equation based reconstruction algorithm for bioluminescence tomography was proposed in this paper. In the algorithm, the third-order simplified spherical harmonics approximation (SP{sub 3}) was combined with the radiosity equation to describe the bioluminescent light propagation in tissues, which provided acceptable accuracy for the turbid medium with both low- and non-scattering regions. The performance of the algorithm was evaluated with digital mouse based simulations and a gastric cancer-bearing mouse based in situ experiment. Primary results demonstrated the feasibility and superiority of the proposed algorithm for the turbid medium with low- and non-scattering regions.

  15. High-quality permanent draft genome sequence of Bradyrhizobium sp. Th.b2, a microsymbiont of Amphicarpaea bracteata collected in Johnson City, New York

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tian, Rui; Parker, Matthew; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Baeshen, Mohammed N.; Baeshen, Nabih A.; et al

    2015-05-16

    Bradyrhizobium sp. Th.b2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing root nodule of Amphicarpaea bracteata collected in Johnson City, New York. Here we describe the features of Bradyrhizobium sp. Th.b2, together with high-quality permanent draft genome sequence information and annotation. The 10,118,060 high-quality draft genome is arranged in 266 scaffolds of 274 contigs, contains 9,809 protein-coding genes and 108 RNA-only encoding genes. In conclusion, this rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

  16. Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light–dark cycle

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Welkie, David; Zhang, Xiaohui; Markillie, Meng; Taylor, Ronald; Orr, Galya; Jacobs, Jon; Bhide, Ketaki; Thimmapuram, Jyothi; Gritsenko, Marina; Mitchell, Hugh; et al

    2014-12-29

    Cyanothece sp. PCC 7822 is an excellent cyanobacterial model organism with great potential to be applied as a biocatalyst for the production of high value compounds. Like other unicellular diazotrophic cyanobacterial species, it has a tightly regulated metabolism synchronized to the light-dark cycle. Utilizing transcriptomic and proteomic methods, we were able to quantify the relationships between transcription and translation underlying central and secondary metabolism in response to nitrogen free, 12 hour light and 12 hour dark conditions.

  17. Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal lightdark cycle

    SciTech Connect (OSTI)

    Welkie, David; Zhang, Xiaohui; Markillie, Meng; Taylor, Ronald; Orr, Galya; Jacobs, Jon; Bhide, Ketaki; Thimmapuram, Jyothi; Gritsenko, Marina; Mitchell, Hugh; Smith, Richard D; Sherman, Louis A

    2014-12-29

    Cyanothece sp. PCC 7822 is an excellent cyanobacterial model organism with great potential to be applied as a biocatalyst for the production of high value compounds. Like other unicellular diazotrophic cyanobacterial species, it has a tightly regulated metabolism synchronized to the light-dark cycle. Utilizing transcriptomic and proteomic methods, we were able to quantify the relationships between transcription and translation underlying central and secondary metabolism in response to nitrogen free, 12 hour light and 12 hour dark conditions.

  18. Modulation of medium-chain fatty acid synthesis in Synechococcus sp. PCC 7002 by replacing FabH with a Chaetoceros Ketoacyl-ACP synthase

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gu, Huiya; Jinkerson, Robert E.; Davies, Fiona K.; Sisson, Lyle A.; Schneider, Philip E.; Posewitz, Matthew C.

    2016-05-26

    The isolation or engineering of algal cells synthesizing high levels of medium-chain fatty acids (MCFAs) is attractive to mitigate the high clouding point of longer chain fatty acids in algal based biodiesel. To develop a more informed understanding of MCFA synthesis in photosynthetic microorganisms, we isolated several algae from Great Salt Lake and screened this collection for MCFA accumulation to identify strains naturally accumulating high levels of MCFA. A diatom, Chaetoceros sp. GSL56, accumulated particularly high levels of C14 (up to 40%), with the majority of C14 fatty acids allocated in triacylglycerols. Using whole cell transcriptome sequencing and de novomore » assembly, putative genes encoding fatty acid synthesis enzymes were identified. Enzymes from this Chaetoceros sp. were expressed in the cyanobacterium Synechococcus sp. PCC 7002 to validate gene function and to determine whether eukaryotic enzymes putatively lacking bacterial evolutionary control mechanisms could be used to improve MCFA production in this promising production strain. Replacement of the Synechococcus 7002 native FabH with a Chaetoceros ketoacyl-ACP synthase Ill increased MCFA synthesis up to fivefold. In conclusion, the level of increase is dependent on promoter strength and culturing conditions.« less

  19. ARM - Datastreams - sp2

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Datastreamssp2 Documentation Data Quality Plots Citation DOI: 10.5439/1182118

  20. Written testimony SP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Written Testimony of Stewart Prager Director, Princeton Plasma Physics Laboratory Professor of Astrophysical Sciences Princeton University Delivered to the Committee on Science, Space and Technology Subcommittee on Energy For the hearing on April 20, 2016 Thank you for the opportunity to appear before this subcommittee and to offer testimony on the Princeton Plasma Physics Laboratory, PPPL (its mission, current activities, and future opportunities) and the ITER project (its importance and

  1. Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici

    SciTech Connect (OSTI)

    Duplessis, Sebastien; Cuomo, Christina A.; Lin, Yao-Cheng; Aerts, Andrea; Tisserant, Emilie; Veneault-Fourrey, Claire; Joly, David L.; Hacquard, Stephane; Amselem, Joelle; Cantarel, Brandi; Chiu, Readman; Couthinho, Pedro; Feau, Nicolas; Field, Matthew; Frey, Pascal; Gelhaye, Eric; Goldberg, Jonathan; Grabherr, Manfred; Kodira, Chinnappa; Kohler, Annegret; Kues, Ursula; Lindquist, Erika; Lucas, Susan; Mago, Rohit; Mauceli, Evan; Morin, Emmanuelle; Murat, Claude; Pangilinan, Jasmyn L.; Park, Robert; Pearson, Matthew; Quesneville, Hadi; Rouhier, Nicolas; Sakthikumar, Sharadha; Salamov, Asaf A.; Schmutz, Jeremy; Selles, Benjamin; Shapiro, Harris; Tangay, Philippe; Tuskan, Gerald A.; Peer, Yves Van de; Henrissat, Bernard; Rouze, Pierre; Ellis, Jeffrey G.; Dodds, Peter N.; Schein, Jacqueline E.; Zhong, Shaobin; Hamelin, Richard C.; Grigoriev, Igor V.; Szabo, Les J.; Martin1, Francis

    2011-04-27

    Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101 mega base pair genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89 mega base pair genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,841 predicted proteins of M. larici-populina to the 18,241 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic life-style include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins (SSPs), impaired nitrogen and sulfur assimilation pathways, and expanded families of amino-acid, oligopeptide and hexose membrane transporters. The dramatic upregulation of transcripts coding for SSPs, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergent adaptation to a biotrophic existence inside plant cells

  2. Integrated in silico analyses of regulatory and metabolic networks of Synechococcus sp. PCC 7002 reveal relationships between gene centrality and essentiality

    SciTech Connect (OSTI)

    Song, Hyun-Seob; McClure, Ryan S.; Bernstein, Hans C.; Overall, Christopher C.; Hill, Eric A.; Beliaev, Alex S.

    2015-03-27

    Cyanobacteria dynamically relay environmental inputs to intracellular adaptations through a coordinated adjustment of photosynthetic efficiency and carbon processing rates. The output of such adaptations is reflected through changes in transcriptional patterns and metabolic flux distributions that ultimately define growth strategy. To address interrelationships between metabolism and regulation, we performed integrative analyses of metabolic and gene co-expression networks in a model cyanobacterium, Synechococcus sp. PCC 7002. Centrality analyses using the gene co-expression network identified a set of key genes, which were defined here as ‘topologically important.’ Parallel in silico gene knock-out simulations, using the genome-scale metabolic network, classified what we termed as ‘functionally important’ genes, deletion of which affected growth or metabolism. A strong positive correlation was observed between topologically and functionally important genes. Functionally important genes exhibited variable levels of topological centrality; however, the majority of topologically central genes were found to be functionally essential for growth. Subsequent functional enrichment analysis revealed that both functionally and topologically important genes in Synechococcus sp. PCC 7002 are predominantly associated with translation and energy metabolism, two cellular processes critical for growth. This research demonstrates how synergistic network-level analyses can be used for reconciliation of metabolic and gene expression data to uncover fundamental biological principles.

  3. Integrated in silico analyses of regulatory and metabolic networks of Synechococcus sp. PCC 7002 reveal relationships between gene centrality and essentiality

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Song, Hyun-Seob; McClure, Ryan S.; Bernstein, Hans C.; Overall, Christopher C.; Hill, Eric A.; Beliaev, Alex S.

    2015-03-27

    Cyanobacteria dynamically relay environmental inputs to intracellular adaptations through a coordinated adjustment of photosynthetic efficiency and carbon processing rates. The output of such adaptations is reflected through changes in transcriptional patterns and metabolic flux distributions that ultimately define growth strategy. To address interrelationships between metabolism and regulation, we performed integrative analyses of metabolic and gene co-expression networks in a model cyanobacterium, Synechococcus sp. PCC 7002. Centrality analyses using the gene co-expression network identified a set of key genes, which were defined here as ‘topologically important.’ Parallel in silico gene knock-out simulations, using the genome-scale metabolic network, classified what we termedmore » as ‘functionally important’ genes, deletion of which affected growth or metabolism. A strong positive correlation was observed between topologically and functionally important genes. Functionally important genes exhibited variable levels of topological centrality; however, the majority of topologically central genes were found to be functionally essential for growth. Subsequent functional enrichment analysis revealed that both functionally and topologically important genes in Synechococcus sp. PCC 7002 are predominantly associated with translation and energy metabolism, two cellular processes critical for growth. This research demonstrates how synergistic network-level analyses can be used for reconciliation of metabolic and gene expression data to uncover fundamental biological principles.« less

  4. Overexpression, purification, crystallization and preliminary X-ray cystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1

    SciTech Connect (OSTI)

    Akioka, Makoto [Department of Applied Biochemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Kyoto 606-8522 (Japan); Nakano, Hiroaki [Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo, Kyoto 606-8501 (Japan); Horikiri, Aya; Tsujimoto, Yoshiyuki; Matsui, Hiroshi [Department of Applied Biochemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Kyoto 606-8522 (Japan); Shimizu, Tetsuya; Nakatsu, Toru; Kato, Hiroaki [Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo, Kyoto 606-8501 (Japan); Watanabe, Kunihiko, E-mail: kwatanab@kpu.ac.jp [Department of Applied Biochemistry, Kyoto Prefectural University, Shimogamo, Sakyo, Kyoto 606-8522 (Japan)

    2006-12-01

    Preliminary X-ray crystallographic study of a proline-specific aminopepitdase from Aneurinibacillus sp, strain AM-1 was carried out. To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2{sub 1}2{sub 1}2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 . A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 202.1 from the MAD data set from the SeMet-substituted crystal were used for phase determination.

  5. Evaluation of the 1998 Predictions of the Run-Timing of Wild Migrant Yearling Chinook and Water Quality at Multiple Locations on the Snake and Columbia Rivers using CRiSP/RealTime, 1998 Technical Report.

    SciTech Connect (OSTI)

    Beer, W. Nicholas; Hayes, Joshua A.; Shaw, Pamela

    1999-07-21

    Since 1988, wild salmon have been PIT-tagged through monitoring and research programs conducted by the Columbia River fisheries agencies and Tribes. Workers at the University of Washington have used detection data at Lower Granite Dam to generate predictions of arrival distributions for various stocks at the dam. The prediction tool is known as RealTime. In 1996, RealTime predictions were linked to a downstream migration model, CRiSP.1. The composite model, known as CRiSP/RealTime, predicts the arrival distribution and fraction transported at downriver locations.

  6. High-quality permanent draft genome sequence of Bradyrhizobium sp. Ai1a-2; a microsymbiont of Andira inermis discovered in Costa Rica

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tian, Rui; Parker, Matthew; Seshadri, Rekha; Reddy, T. B. K.; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Woyke, Tanja; Baeshen, Mohammed; Baeshen, Nabih; et al

    2015-06-14

    Bradyrhizobium sp. Ai1a-2 is is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen fixing root nodule of Andira inermis collected from Tres Piedras in Costa Rica. In this report we describe, for the first time, the genome sequence information and annotation of this legume microsymbiont. The 9,029,266 bp genome has a GC content of 62.56% with 247 contigs arranged into 246 scaffolds. The assembled genome contains 8,482 protein-coding genes and 102 RNA-only encoding genes. Lastly, this rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Rootmore » Nodule Bacteria (GEBA-RNB) project proposal.« less

  7. Fibroblast growth factor-2 up-regulates the expression of nestin through the RasRafERKSp1 signaling axis in C6 glioma cells

    SciTech Connect (OSTI)

    Chang, Kai-Wei; Huang, Yuan-Li; Wong, Zong-Ruei; Su, Peng-Han; Huang, Bu-Miin; Ju, Tsai-Kai; Technology Commons, College of Life Science, National Taiwan University, Taipei 106, Taiwan ; Yang, Hsi-Yuan

    2013-05-17

    Highlights: Nestin expression in C6 glioma cells is induced by FGF-2. Nestin expression is induced by FGF-2 via de novo RNA and protein synthesis. The FGFR inhibitor SU5402 blocks the FGF-2-induced nestin expression. The mRNA of FGFR1 and 3 are detected in C6 glioma cells. RasRafERKSp1 signaling pathway is responsibe for FGF-2-induced nestin expression. -- Abstract: Nestin is a 240-kDa intermediate filament protein expressed mainly in neural and myogenic stem cells. Although a substantial number of studies have focused on the expression of nestin during development of the central nervous system, little is known about the factors that induce and regulate its expression. Fibroblast growth factor-2 (FGF-2) is an effective mitogen and stimulates the proliferation and differentiation of a subset of nestin-expressing cells, including neural progenitor cells, glial precursor cells, and smooth muscle cells. To assess whether FGF-2 is a potent factor that induces the expression of nestin, C6 glioma cells were used. The results showed that nestin expression was up-regulated by FGF-2 via de novo RNA and protein synthesis. Our RT-PCR results showed that C6 glioma cells express FGFR1/3, and FGFRs is required for FGF-2-induced nestin expression. Further signaling analysis also revealed that FGF-2-induced nestin expression is mediated through FGFRMAPKERK signaling axis and the transcriptional factor Sp1. These findings provide new insight into the regulation of nestin in glial system and enable the further studies on the function of nestin in glial cells.

  8. Structure of a highly acidic β-lactamase from the moderate halophile Chromohalobacter sp. 560 and the discovery of a Cs{sup +}-selective binding site

    SciTech Connect (OSTI)

    Arai, Shigeki; Yonezawa, Yasushi; Okazaki, Nobuo; Matsumoto, Fumiko; Shibazaki, Chie; Shimizu, Rumi; Yamada, Mitsugu; Adachi, Motoyasu; Tamada, Taro; Kawamoto, Masahide; Tokunaga, Hiroko; Ishibashi, Matsujiro; Blaber, Michael; Tokunaga, Masao; Kuroki, Ryota

    2015-03-01

    The tertiary structure of a β-lactamase derived from the halobacterium Chromohalobacter sp. 560 (HaBLA) was determined by X-ray crystallography. Three unique Sr{sup 2+}-binding sites and one Cs{sup +}-binding site were discovered in the HaBLA molecule. Environmentally friendly absorbents are needed for Sr{sup 2+} and Cs{sup +}, as the removal of the radioactive Sr{sup 2+} and Cs{sup +} that has leaked from the Fukushima Nuclear Power Plant is one of the most important problems in Japan. Halophilic proteins are known to have many acidic residues on their surface that can provide specific binding sites for metal ions such as Cs{sup +} or Sr{sup 2+}. The crystal structure of a halophilic β-lactamase from Chromohalobacter sp. 560 (HaBLA) was determined to resolutions of between 1.8 and 2.9 Å in space group P3{sub 1} using X-ray crystallography. Moreover, the locations of bound Sr{sup 2+} and Cs{sup +} ions were identified by anomalous X-ray diffraction. The location of one Cs{sup +}-specific binding site was identified in HaBLA even in the presence of a ninefold molar excess of Na{sup +} (90 mM Na{sup +}/10 mM Cs{sup +}). From an activity assay using isothermal titration calorimetry, the bound Sr{sup 2+} and Cs{sup +} ions do not significantly affect the enzymatic function of HaBLA. The observation of a selective and high-affinity Cs{sup +}-binding site provides important information that is useful for the design of artificial Cs{sup +}-binding sites that may be useful in the bioremediation of radioactive isotopes.

  9. Draft Genome Sequence of Pseudoalteromonas sp. Strain ND6B, an Oil-Degrading Isolate from Eastern Mediterranean Sea Water Collected at a Depth of 1,210 Meters

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Harris, Austin P.; Techtmann, Stephen M.; Stelling, Savannah C.; Utturkar, Sagar M.; Alshibli, Noor K.; Brown, Steven D.; Hazen, Terry C.

    2014-11-26

    We report the draft genome of Pseudoalteromonas sp. strain ND6B, which is able to grow with crude oil as a carbon source. Strain ND6B was isolated from eastern Mediterranean Sea deep water at a depth of 1,210 m. The genome of strain ND6B provides insight into the oil-degrading ability of the Pseudoalteromonas species.

  10. The Dark Side of Algae Cultivation. Characterizing night biomass loss in three photosynthetic algae, Chlorella sorokiniana, Nannochloropsis salina and Picochlorum sp

    SciTech Connect (OSTI)

    Edmundson, Scott J.; Huesemann, Michael H.

    2015-10-28

    Night biomass loss in photosynthetic algae is an essential parameter that is often overlooked when modeling or optimizing biomass productivities. Night respiration acts as a tax on daily biomass gains and has not been well characterized in the context of biofuel production. We examined the night biomass loss in three algae strains that may have potential for commercial biomass production (Nannochloropsis salina- CCMP1776, Chlorella sorokiniana- DOE1412, and Picochlorum sp. LANL-WT). Biomass losses were monitored by ash free dry weight (AFDW mg/L-1) and optical density (OD750) on a thermal-gradient incubator. Night biomass loss rates were highly variable (ranging from -0.006 to -0.59 day -1), species-specific, and dependent on both culture growth phase prior to the dark period and night pond temperature. In general, the fraction of biomass lost over a 10 hour dark period, which ranged from ca. 1 to 22% in our experiments, was positively correlated with temperature and declined as the culture transitioned from exponential to linear to stationary phase. The dynamics of biomass loss should be taken into consideration in algae strain selection, are critical in predictive modeling of biomass production based on geographic location and can influence the net productivity of photosynthetic cultures used for bio-based fuels or products.

  11. Enhancing photo-catalytic production of organic acids in the cyanobacterium S ynechocystis sp. PCC 6803 Δ glg C , a strain incapable of glycogen storage

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Carrieri, Damian; Broadbent, Charlie; Carruth, David; Paddock, Troy; Ungerer, Justin; Maness, Pin-Ching; Ghirardi, Maria; Yu, Jianping

    2015-01-23

    We describe how a key objective in microbial biofuels strain development is to maximize carbon flux to target products while minimizing cell biomass accumulation, such that ideally the algae and bacteria would operate in a photo-catalytic state. A brief period of such a physiological state has recently been demonstrated in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC strain incapable of glycogen storage. When deprived of nitrogen, the ΔglgC excretes the organic acids alpha-ketoglutarate and pyruvate for a number of days without increasing cell biomass. This study examines the relationship between the growth state and the photo-catalytic state, and characterizes themore » metabolic adaptability of the photo-catalytic state to increasing light intensity. It is found that the culture can transition naturally from the growth state into the photo-catalytic state when provided with limited nitrogen supply during the growth phase. Photosynthetic capacity and pigments are lost over time in the photo-catalytic state. Reversal to growth state is observed with re-addition of nitrogen nutrient, accompanied by restoration of photosynthetic capacity and pigment levels in the cells. While the overall productivity increased under high light conditions, the ratio of alpha-ketoglutarate/pyruvate is altered, suggesting that carbon partition between the two products is adaptable to environmental conditions.« less

  12. Multi-omic dynamics associate oxygenic photosynthesis with nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142

    SciTech Connect (OSTI)

    Bernstein, Hans C.; Charania, Moiz A.; McClure, Ryan S.; Sadler, Natalie C.; Melnicki, Matthew R.; Hill, Eric A.; Markillie, Lye Meng; Nicora, Carrie D.; Wright, Aaron T.; Romine, Margaret F.; Beliaev, Alexander S.

    2015-11-03

    This study combines transcriptomic and proteomic profiling to provide new insights on the metabolic relationship between oxygenic photosynthesis and nitrogenase-mediated H2 production in the model cyanobacterium, Cyanothece sp. ATCC 51142. To date, the proposed mechanisms used to describe the energy metabolism processes that support H2 production in Cyanothece 51142 have assumed that ATP and reductant requirements are derived solely from glycogen oxidation and/or cyclic-electron flow around photosystem I. The results from this study present and test an alternative hypothesis by showing that net-positive rates of oxygenic photosynthesis and increased expression of photosystem II reaction centers correspond and are synchronized with nitrogenase expression and H2 production. These findings provide a new and more complete view on the metabolic processes contributing to the energy budget of photosynthetic H2 production and highlight the likely role of photocatalytic H2O oxidation as a major participating process.

  13. Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002

    SciTech Connect (OSTI)

    Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.; Bryant, Donald A.; Peters, John W.

    2014-10-18

    The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel production. We demonstrate the growth of C. reinhardtii on acetate provided by mutant strains of the cyanobacterium Synechococcus sp. PCC7002.

  14. Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia

    SciTech Connect (OSTI)

    Cole, Jesse; Gieler, Brandon; Heisler, Devon; Palisoc, Maryknoll; Williams, Amanda; Dohnalkova, Alice; Ming, Hong; Yu, Tian T.; Dodsworth, Jeremy A.; Li, Wen J.; Hedlund, Brian P.

    2013-08-15

    Several closely-related, thermophilic, and cellulolytic bacterial strains, designated JKG1T, JKG2, JKG3, JKG4, and JKG5, were isolated from a cellulolytic enrichment (corn stover) incubated in the water column of Great Boiling Spring, NV. Strain JKG1T had cells of a diameter of 0.7 - 0.9 ?m and length of ~2.0 ?m that formed non-branched multicellular filaments reaching >300 ?m. Spores were not formed and dense liquid cultures were red. The temperature range for growth was 45-65 C, with an optimum of 55 C. The pH range for growth was 5.6-9.0, with an optimum of 7.5. JKG1T grew as an aerobic heterotroph, utilizing glucose, sucrose, xylose, arabinose, cellobiose, carboxymethylcellulose, filter paper, microcrystalline cellulose, xylan, starch, casamino acids, tryptone, peptone, yeast extract, acetate, citrate, lactate, pyruvate, and glycerol as sole carbon sources, and was not observed to photosynthesize. The cells stained Gram-negative. Phylogenetic analysis using 16S rRNA gene sequences placed the new isolates in the class Chloroflexia, but distant from other cultivated members, with the highest sequence identity of 82.5% to Roseiflexus castenholzii. The major quinone was menaquinone-9; no ubiquinones were detected. The major cellular fatty acids (>5%) were C18:0, anteiso-C17:0, iso-C18:0, and iso-C17:0. C16:0, iso-C16:0, and C17:0. The peptidoglycan amino acids were alanine, ornithine, glutamic acid, serine, and asparagine. Whole-cell sugars included mannose, rhamnose, glucose, galactose, ribose, arabinose, and xylose. Morphological, phylogenetic, and chemotaxonomic results suggest that JKG1T is representative of a new lineage within the class Chloroflexia, which we propose to designate Kallotenue papyrolyticum gen. nov., sp. nov., Kallotenuaceae fam. nov., Kallotenuales ord. nov.

  15. MOU_DOE_US_SPAIN.pdf

    Office of Environmental Management (EM)

    MA-60 Org chart MA-60 Org chart Updated May 5, 2016 OAM Org Chart (MA-60) 053016.png OAM Org Chart (MA-60) 053016.pdf (148.11 KB) Every Region | Department of Energy

    MAP: How Climate Change Threatens America's Energy Infrastructure in Every Region MAP: How Climate Change Threatens America's Energy Infrastructure in Every Region October 9, 2015 - 10:28am Addthis This interactive map is not viewable in your browser. Please view it in a modern browser. Daniel Wood Daniel Wood Data

  16. Madrid, Spain: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    S A Control Y Montages Industriales CYMI SA Corporacion Eolica CESA SA DAVID Fuel Cell Components SL Dhamma Energy E ON Renovables Iberia formerly Energi E2 Renovables...

  17. Fig. 20.1.sp

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  18. Fig. 20.2.sp

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  19. Fig. 20.3.sp

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  20. Fig. 20.5.sp

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  1. Titan_SP-1.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Tips For Getting Hired Tips For Getting Hired Successful candidates know it takes a little extra effort to get to the top. This one hour workshop introduces you to the Department of Energy, in addition to guiding you in creating a competitive Federal resume, it also covers veteran hiring options, and offers important tips to help prepare for the interview. So, If you are new to the Federal government hiring process or just want to improve your odds of getting that critical interview, this

  2. Accumulation of Pb and Cu heavy metals in sea water, sediment, and leaf and root tissue of Enhalus sp. in the seagrass bed of Banten Bay

    SciTech Connect (OSTI)

    Fauziah, Faiza Choesin, Devi N.

    2014-03-24

    Banten Bay in Indonesia is a coastal area which has been highly affected by human activity. Previous studies have reported the presence of lead (Pb) and copper (Cu) heavy metals in the seawater of this area. This study was conducted to measure the accumulation of Pb and Cu in seawater, sediment, leaf tissue, and root tissue of the seagrass species Enhalus sp. Sampling was conducted at two observation stations in Banten Bay: Station 1 (St.1) was located closer to the coastline and to industrial plants as source of pollution, while Station 2 (St.2) was located farther away offshore. At each station, three sampling points were established by random sampling. Field sampling was conducted at two different dates, i.e., on 29 May 2012 and 30 June 2012. Samples were processed by wet ashing using concentrated HNO{sub 3} acid and measured using Atomic Absorption Spectrometry (AAS). Accumulation of Pb was only detected in sediment samples in St.1, while Cu was detected in all samples. Average concentrations of Cu in May were as follows: sediment St.1 = 0.731 ppm, sediment St.2 = 0.383 ppm, seawater St.1 = 0.163 ppm, seawater St.2 = 0.174 ppm, leaf St.1 = 0.102 ppm, leaf St.2 = 0.132 ppm, root St.1= 0.139 ppm, and root St.2 = 0.075 ppm. Average measurements of Cu in June were: sediment St.1 = 0.260 ppm, leaf St.1 = 0.335 ppm, leaf St.2 = 0.301 ppm, root St.1= 0.047 ppm, and root St.2 = 0.060 ppm. In June, Cu was undetected in St.2 sediment and seawater at both stations. In May, Cu concentration in seawater exceeded the maximum allowable threshold for water as determined by the Ministry of the Environment. Spatial and temporal variation in Pb and Cu accumulation were most probably affected by distance from source and physical conditions of the environment (e.g., water current and mixing)

  3. Spain-Chile and Spain-Ecuador cooperation in the field of research nuclear reactors

    SciTech Connect (OSTI)

    Avendano, G.; Rodriguez, M.L.; Manas, L.; Masalleras, E.; Montes, J.

    1981-01-01

    The Spanish Board of Nuclear Energy (JEN) has been cooperating for the last several years with the Comision Chilena de Energia Nuclear (Chilean Commission of Nuclear Energy (CCHEN)), on the one hand, and with the Comision Ecuatoriana de Energia Atomica (Ecuadorian Commission of Atomic Energy (CEEA)), on the other. The result of this cooperation has been the implementation of projects in both countries to create research centers around a nuclear reactor as the main working tool: the Lo Aguirre reactor in Chile and the Ruminahui reactor in Ecuador.

  4. Cis-Chlorobenzene dihydrodiol dehydrogenase (TcbB) from Pseudomonas sp. strain P51, expressed in Escherichia coli DH5{alpha}(pTCB149), catalyzes enantioselective dehydrogenase reactions

    SciTech Connect (OSTI)

    Raschke, H.; Fleischmann, T.; Meer, J.R. van der; Kohler, H.P.E.

    1999-12-01

    cis-Chlorobenzene dihydrodiol dehydrogenase (CDD) from Pseudomonas sp. strain P51, cloned into Escherichia coli DH5{alpha}(pTCB149) was able to oxidize cis-dihydrodihydroxy derivatives (cis-dihydrodiols) of dihydronaphthalene, indene, and four para-substituted toluenes to the corresponding catechols. During the incubation of a nonracemic mixture of cis-1,2-indandiol, only the (+)-cis-(1R,2S) enantiomer was oxidized; the (-)-cis-(S,2R) enantiomer remained unchanged, CDD oxidized both enantiomers of cis-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene, but oxidation of the (+)-cis-(1S,2R) enantiomer was delayed until the (-)-cis-(1R,2S) enantiomer was completely depleted. When incubated with nonracemic mixtures of para-substituted cis-toluene dihydrodiols, CDD always oxidized the major enantiomer at a higher rate than the minor enantiomer. When incubated with racemic 1-indanol, CDD enantioselectively transformed the (+)-(1S) enatiomer to 1-indanone. This stereoselective transformation shows that CDD also acted as an alcohol dehydrogenase. Additionally, CDD was able to oxidize (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene, (+)-cis-monochlorobiphenyl dihydrodiols, and (+)-cis-toluene dihydrodiol to the corresponding catechols.

  5. Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov.

    SciTech Connect (OSTI)

    Dodsworth, Jeremy A.; Gevorkian, Jonathan; Despujos, Fairuz; Cole, Jesse; Murugapiran, Senthil K.; Ming, Hong; Li, Wen J.; Zhang, Gengxin; Dohnalkova, Alice; Hedlund, Brian P.

    2014-06-06

    A thermophilic, filamentous, heterotrophic bacterium designated strain JAD2T was isolated from sediment of Great Boiling Spring in Nevada, USA. Cells had an average diameter of 0.3 m and length of 4.0 m, and formed filaments typically ranging in length from 20 m to 200 m. Filaments were negative for the Gram stain reaction, spores were not formed, and motility was not observed. The optimum temperature for growth was 75 C with a range from 67.5-75 C, and the optimum pH for growth was 6.75 with a range from 6.5-7.75. Peptone, tryptone or yeast extract were able to support growth when supplemented with a vitamin solution, but no growth was observed using a variety of defined organic substrates. Strain JAD2T was a facultative microaerophile, with optimal growth at 1% v/v O2 and an upper limit of 8% O2, and anaerobic growth was stimulated by fumarate but inhibited by sulfite and elemental sulfur. The major cellular fatty acids (>5%) were C16:0, C19:0, C18:0, C20:0, and C19:1. The genomic DNA G+C content was 69.3%. Phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and other conserved genes placed JAD2T and other members of the yet-uncultivated GAL35 group within the phylum Chloroflexi, but not within any existing class in this phylum. These results indicate that strain JAD2T is the first cultivated representative of a new lineage within the phylum Chloroflexi, for which we propose the name Thermoflexus hugenholtzii gen. nov., sp. nov., type strain JAD2T, within Thermoflexia classis nov., Thermoflexales ord. nov., and Thermoflexaceae fam. nov.

  6. Sorgenia SpA | Open Energy Information

    Open Energy Info (EERE)

    Services Product: Sorgenia produces electricity from both renewable energy sources and natural gas. It also buys and sells electricity and gas in the market and provides energy...

  7. IMAT SpA | Open Energy Information

    Open Energy Info (EERE)

    33074 Sector: Renewable Energy, Solar Product: Italy-based company specializing in manufacturing of components for household refrigeration. Their main renewable energy focus is...

  8. Microsoft Word - 2012_sp_04.docx

    U.S. Energy Information Administration (EIA) Indexed Site

    ... For example, even though the population of the East North Central Census region increased from 41 million to 46 million between 1975 (the start of the STEO database) and 2011, that ...

  9. Microsoft Word - 2013_sp_05.docx

    U.S. Energy Information Administration (EIA) Indexed Site

    Estimates of Crude Oil and Liquid Fuels Supply Disruptions 1 September 10, 2013 Short-Term Energy Outlook Supplement: EIA Estimates of Crude Oil and Liquid Fuels Supply Disruptions 1 Unplanned crude oil and liquid fuels supply disruptions may occur frequently in many countries and for a variety of reasons, including conflicts, natural disasters, and technical difficulties. Although crude oil and liquid fuels supply disruptions may occur at any time, recent outages have particularly unsettled the

  10. Microsoft Word - 2011_sp_02.docx

    Gasoline and Diesel Fuel Update (EIA)

    Independent Statistics & Analysis U.S. Energy Information Administration June 2011 Short-Term Energy Outlook Supplement: 2011 Outlook for Hurricane-Related Production Outages in the Gulf of Mexico Highlights  The National Oceanic and Atmospheric Administration's (NOAA) Atlantic Hurricane Season Outlook, released on May 19, 2011, predicts that the Atlantic basin likely will experience above-normal tropical weather activity during this year's hurricane season (June 1 - November 30). 1 NOAA

  11. Microsoft Word - 2012_sp_02.docx

    Gasoline and Diesel Fuel Update (EIA)

    Brent Crude Oil Spot Price Forecast 1 July 10, 2012 Short-Term Energy Outlook Supplement: Brent Crude Oil Spot Price Forecast Since the mid-1980s, benchmark crude oil prices such as West Texas Intermediate (WTI) in the United States and Brent crude oil in Europe have served as reference points that the market uses for pricing other crude oils. The historically close relationships between the major benchmarks made WTI prices a reliable indicator of the average cost of crude oil to U.S. refiners,

  12. Microsoft Word - 2013_sp_02.docx

    Gasoline and Diesel Fuel Update (EIA)

    Change in Regional and U.S. Degree-Day Calculations 1 September 11, 2012 Short-Term Energy Outlook Supplement: Change in Regional and U.S. Degree-Day Calculations Average daily temperatures during the summer and winter months, reported as population- weighted cooling degree days (CDDs) and heating degree days (HDDs), respectively, are important in explaining and forecasting the consumption of energy for space cooling and heating in the U.S. Energy Information Administration's Short-Term Energy

  13. Microsoft Word - 2011_sp_01.docx

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Notice - June 2011 Independent Statistics & Analysis U.S. Energy Information Administration June 2011 Short-Term Energy Outlook Notice: Suspension of Regional Residential Heating ...

  14. HiperDNO (Smart Grid Project) (Spain) | Open Energy Information

    Open Energy Info (EERE)

    is to develop a new generation of distribution network management systems that exploits novel near to realtime HPC solutions with inherent security and intelligent communications...

  15. Spain Installed Wind Capacity Website | Open Energy Information

    Open Energy Info (EERE)

    URI: cleanenergysolutions.orgcontentspain-installed-wind-capacity-website Language: English Policies: Regulations Regulations: Feed-in Tariffs This website presents an...

  16. Cameron, LA Liquefied Natural Gas Exports Price to Spain (Dollars...

    U.S. Energy Information Administration (EIA) Indexed Site

    Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 2000's -- -- -- 2010's -- 7.58 -- -- --

  17. Once perks' poster child, Spain slashes subsidies for solar energy

    SciTech Connect (OSTI)

    2009-11-15

    Spanish solar subsidies peaked at $1.6 billion in 2008 from $310 million in 2007. Towards the end of 2008, the government slashed the subsidies and put a 500 MW annual limit on how much new solar installations it would take.

  18. EUDEEP (Smart Grid Project) (Spain) | Open Energy Information

    Open Energy Info (EERE)

    technical and nontechnical barriers that prevent a massive deployment of distributed energy resources (DER) in Europe. In partnership with manufacturers, research organizations,...

  19. FY 2016 SES and SP Opening Guidance Memo_0.pdf

    Office of Environmental Management (EM)

  20. Solution Package Scope Definition, Report 72, Salt Waste (SP #72)

    Broader source: Energy.gov [DOE]

    Supporting Technical Document for the Radiological Release Accident Investigation Report (Phase II Report)

  1. The [NiFe]-hydrogenase of the cyanobacterium Synechocystis sp...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PCC 6803 works bidirectionally with a bias to H2 production Authors: McIntosh, C. L., ... can be easily monitored as current under rapidly changing experimental conditions. ...

  2. SP-100 Program: space reactor system and subsystem investigations

    SciTech Connect (OSTI)

    Harty, R.B.

    1983-09-30

    For a space reactor power system, a comprehensive safety program will be required to assure that no undue risk is present. This report summarizes the nuclear safety review/approval process that will be required for a space reactor system. The documentation requirements are presented along with a summary of the required contents of key documents. Finally, the aerospace safety program conducted for the SNAP-10A reactor system is summarized. The results of this program are presented to show the type of program that can be expected and to provide information that could be usable in future programs.

  3. sp3-hybridized framework structure of group-14 elements discovered...

    Office of Scientific and Technical Information (OSTI)

    with energy band gaps close to the optimal values for optoelectronic or photovoltaic applications. With metal-atom encapsulation, the P42mnm structure would also be a...

  4. Solar Ventures SpA | Open Energy Information

    Open Energy Info (EERE)

    Zip: 20122 Sector: Solar Product: Solar PV project developer investor, focused on the Italian market and considering projects in Greece and France. References: Solar Ventures...

  5. Caloramator sp. Tolerance of Pretreatment Inhibitors from Lignocellulosics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Calling All Fuel Cells Calling All Fuel Cells December 7, 2012 - 4:31pm Addthis Altergy had more than 60 fuel cells in the immediate Hurricane Sandy disaster area that acted as backup power for cell phone towers. | Photo courtesy of Altergy. Altergy had more than 60 fuel cells in the immediate Hurricane Sandy disaster area that acted as backup power for cell phone towers. | Photo courtesy of Altergy. Sunita Satyapal Director, Fuel Cell Technologies Office What is a fuel cell? A fuel cell is a

  6. Microsoft Word - FY 2015 SP Performance Appraisal System Guidance...

    Broader source: Energy.gov (indexed) [DOE]

    Office of Executive Resources Office of the Chief Human Capital Officer U.S. Department of ... (5% - 10 %) o Acquires and administers human, financial, material, and information ...

  7. AHSS Stamping Project … A/SP 050; Nonlinear Strain Paths Project … A/SP 061

    Broader source: Energy.gov [DOE]

    2011 DOE Hydrogen and Fuel Cells Program, and Vehicle Technologies Program Annual Merit Review and Peer Evaluation

  8. Current and anticipated uses of thermal-hydraulic codes in Spain

    SciTech Connect (OSTI)

    Pelayo, F.; Reventos, F.

    1997-07-01

    Spanish activities in the field of Applied Thermal-Hydraulics are steadily increasing as the codes are becoming practicable enough to efficiently sustain engineering decision in the Nuclear Power industry. Before reaching this point, a lot of effort has been devoted to achieve this goal. This paper briefly describes this process, points at the current applications and draws conclusions on the limitations. Finally it establishes the applications where the use of T-H codes would be worth in the future, this in turn implies further development of the codes to widen the scope of application and improve the general performance. Due to the different uses of the codes, the applications mainly come from the authority, industry, universities and research institutions. The main conclusion derived from this paper establishes that further code development is justified if the following requisites are considered: (1) Safety relevance of scenarios not presently covered is established. (2) A substantial gain in margins or the capability to use realistic assumptions is obtained. (3) A general consensus on the licensability and methodology for application is reached. The role of Regulatory Body is stressed, as the most relevant outcome of the project may be related to the evolution of the licensing frame.

  9. Price of Liquefied U.S. Natural Gas Re-Exports to Spain (Dollars...

    U.S. Energy Information Administration (EIA) Indexed Site

    Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 2000's -- -- -- 2010's 6.32 7.77 --

  10. Liquefied U.S. Natural Gas Re-Exports to Spain (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 2000's 0 0 0 2010's 4,117 5,918 0

  11. Liquefied U.S. Natural Gas Re-Exports to Spain (Million Cubic...

    U.S. Energy Information Administration (EIA) Indexed Site

    Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2010 0 3,149 0 0 0 0 0 0 0 0 968 0 2011 3,007 0 0 2,911 0 0 0 0 0 0 0 0 2012 0 0 0 0 0 0 0 0 0 0 0 0 2014 0 0 0 0 0 0 0 0 0 0 0 ...

  12. Price of U.S. Liquefied Natural Gas Exports to Spain (Dollars...

    U.S. Energy Information Administration (EIA) Indexed Site

    Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2010 -- 5.69 -- -- -- -- -- -- -- -- 8.39 -- 2011 7.96 -- -- 7.58 -- -- -- -- -- -- -- -- 2015 0.00 0.00 0.00 0.00 0.00 0.00 ...

  13. Price of U.S. Liquefied Natural Gas Exports to Spain (Dollars...

    U.S. Energy Information Administration (EIA) Indexed Site

    Decade Year-0 Year-1 Year-2 Year-3 Year-4 Year-5 Year-6 Year-7 Year-8 Year-9 2000's -- -- -- 2010's 6.32 7.77 -- -- -- --

  14. Cameron, LA Liquefied Natural Gas Exports Price to Spain (Dollars per

    U.S. Energy Information Administration (EIA) Indexed Site

    Thousand Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2011 7.58

  15. U.S. and Spain Equip Second Megaport With Radiation Detectors...

    National Nuclear Security Administration (NNSA)

    implementing President Obama's nuclear security agenda and supports the U.S. global effort to secure vulnerable nuclear material and prevent nuclear proliferation and terrorism." ...

  16. Price Liquefied Sabine Pass, LA Natural Gas Exports Price to Spain (Dollars

    U.S. Energy Information Administration (EIA) Indexed Site

    per Thousand Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2011 7.96

  17. DTOcean 4th General Meeting, Madrid, Spain (10/27-29/2015)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Sandia Labs Participated in CEM7 as Part of a Smart Grid Research Network Permalink International Conference on Integration of Renewable and Distributed Energy Resources (IRED): ...

  18. Price of Liquefied U.S. Natural Gas Re-Exports to Spain (Dollars per

    U.S. Energy Information Administration (EIA) Indexed Site

    Thousand Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2010 -- 5.69 -- -- -- -- -- -- -- -- 8.39 -- 2011 7.96 -- -- 7.58 -- -- -- -- -- -- -- -- 2012 -- -- -- -- -- -- -- -- -- -- -- -- 2013 -- -- -- -- -- -- -- -- -- -- -- -- 2014 -- -- -- -- -- -- -- -- -- -- -- -- 2015 -- -- -- -- -- -- -- -- -- -- -- -- 2016 -- --

  19. Photo-z optimization for measurements of the BAO radial scale...

    Office of Scientific and Technical Information (OSTI)

    i Estudis Avancats, Barcelona (Spain) Instituto de Fisica Corpuscular (CSIC-UVEG), Valencia (Spain) Instituto de Fisica Corpuscular (CSIC-UVEG), Valencia (Spain) Publication ...

  20. A new reversal mode in exchange coupled antiferromagnetic/ferromagneti...

    Office of Scientific and Technical Information (OSTI)

    Universitat Autnoma de Barcelona (Spain). Departament de Fisica Universitat Autnoma de Barcelona (Spain). Departament de Fisica; Universitat Autnoma de Barcelona (Spain). ...

  1. Vehicle Technologies Office Merit Review 2016: E. Coli Derived Spider Silk MaSp1 and MaSp2 Proteins as Carbon Fiber Precursors

    Broader source: Energy.gov [DOE]

    Presentation given by Utah State University at the 2016 DOE Vehicle Technologies Office and Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting about Lightweighting

  2. Environmental screening tools for assessment of infrastructure plans based on biodiversity preservation and global warming (PEIT, Spain)

    SciTech Connect (OSTI)

    Garcia-Montero, Luis G.

    2010-04-15

    Most Strategic Environmental Assessment (SEA) research has been concerned with SEA as a procedure, and there have been relatively few developments and tests of analytical methodologies. The first stage of the SEA is the 'screening', which is the process whereby a decision is taken on whether or not SEA is required for a particular programme or plan. The effectiveness of screening and SEA procedures will depend on how well the assessment fits into the planning from the early stages of the decision-making process. However, it is difficult to prepare the environmental screening for an infrastructure plan involving a whole country. To be useful, such methodologies must be fast and simple. We have developed two screening tools which would make it possible to estimate promptly the overall impact an infrastructure plan might have on biodiversity and global warming for a whole country, in order to generate planning alternatives, and to determine whether or not SEA is required for a particular infrastructure plan.

  3. Depositional sequences and fluvial architecture in the Cameros extension basin, north-central Spain, upper Jurassic-lower cretaceous

    SciTech Connect (OSTI)

    Clemente, P. )

    1993-09-01

    In the Tithonian-Berriasian and Aptian, basin fill of the Cameros basin is formed by a depositional megasequence of fluvial and lacustrine sediments. Basin evolution is related the second state of rifting in the North Atlantic. In the first stages of extension, the basin is compartmentalized due to differential subsidence. As the extension continues, the subbasins merge to form one large basin. The megasequence is subdivided into five unconformity-bounded depositional sequences (SD1-SD5). Each sequence has a duration of 2.5-10 m.y. and a thickness of 400-1200 m. the internal sequence architecture is formed by a thick fluvial depositional system, which toward the top is overlapped by an expanding lacustrine facies. The architecture of the fluvial systems in depositional sequences SD1-SD3 consists of small, isolated sandstone bodies in a mudstone matrix, and results from the evolution of distal, high-sinuosity fluvio-lacustrine coastal plains. Depositional mixed (conglomeratic sandstone) and sandstone bodies. They originate from wider and nonconfined fluvial systems (conglomeratic and sandy braid plains). In SD5 this facies interfingers with a second fluvial system dominated by ephemeral streams. The evolution of fluvial architecture is controlled by the balance between subsidence, sediment supply, and relative sea level change. In a reservoir-equivalent setting, the understanding of this evolutionary process and its resultant architecture provides a better insight in reservoir distribution and interconnectedness.

  4. Smartgrid EV Communication module (SpEC) SAE DC Charging Digital...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DC fast charging enables rapid recharging of electric vehicles along heavy traffic corridors and at public stations. A DC fast charge can add 60 to 80 miles of range to an EV in ...

  5. Carbonaceous Aerosols and Radiative Effects Study (CARES), g1-aircraft, sedlacek sp2

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Sedlacek, Art

    2011-08-30

    The primary objective of the Carbonaceous Aerosol and Radiative Effects Study (CARES) in 2010 was to investigate the evolution of carbonaceous aerosols of different types and their optical and hygroscopic properties in central California, with a focus on the Sacramento urban plume.

  6. Treatment Facility D Krauter, P.W.; Harrar, J.E.; Orloff, S.P...

    Office of Scientific and Technical Information (OSTI)

    Lab., CA (United States) USDOE Assistant Secretary for Environment, Safety, and Health, Washington, DC (United States) United States 1998-01-01 English Technical Report...

  7. FY15 SES SP Performance Appraisal Cycle_Memo to Heads of Departmental _0.pdf

    Office of Environmental Management (EM)

    FY 2016-2018 Action Agenda Framework Webinar FY 2016-2018 Action Agenda Framework Webinar Learn about the EJ IWG's Draft Action Agenda Framework. The Framework advances greater Federal agency collaboration to support holistic community-based solutions to address environmental justice issues in environmental protection, housing, transportation, economic development, energy policy, natural and cultural resources management and health disparities. FY 2016-2018 Action Agenda Framework Webinar

  8. Biofuels from Microalgae: Review of Products, Processes and Potential, with Special Focus on Dunaliella sp.

    SciTech Connect (OSTI)

    Huesemann, Michael H.; Benemann, John R.

    2009-12-31

    There is currently great interest in using microalgae for the production of biofuels, mainly due to the fact that microalgae can produce biofuels at a much higher productivity than conventional plants and that they can be cultivated using water, in particular seawater, and land not competing for resources with conventional agriculture. However, at present such microalgae-based technologies are not yet developed and the economics of such processes are uncertain. We review power generation by direct combustion, production of hydrogen and other fuel gases and liquids by gasification and pyrolysis, methane generation by anaerobic digestion, ethanol fermentations, and hydrogen production by dark and light-driven metabolism. We in particular discuss the production of lipids, vegetable oils and hydrocarbons, which could be converted to biodiesel. Direct combustion for power generation has two major disadvantages in that the high N-content of algal biomass causes unacceptably high NOx emissions and losses of nitrogen fertilizer. Thus, the use of sun-dried microalgal biomass would not be cost-competitive with other solid fuels such as coal and wood. Thermochemical conversion processes such as gasification and pyrolysis have been successfully demonstrated in the laboratory but will be difficult to scale up commercially and suffers from similar, though sometimes not as stringent, limitations as combustion. Anaerobic digestion of microalgal cells yields only about 0.3 L methane per g volatile solids destroyed, about half of the maximum achievable, but yields can be increased by adding carbon rich substrates to circumvent ammonia toxicity caused by the N-rich algal biomass. Anaerobic digestion would be best suited for the treatment of algal biomass waste after value-added products have been separated. Algae can also be grown to accumulate starches or similar fermentable products, and ethanol or similar (e.g., butanol) fermentations could be applied to such biomass, but research is required on increasing solvent yields. Dark fermentation of algal biomass can also produce hydrogen, but, as for other fermentations, only at low yields. Hydrogen can also be generated by algae in the light, however, this process has not yet been demonstrated in any way that could be scaled up and, in any event, Dunaliella, is not known to produce hydrogen. In response to nutrient deficiency (nitrogen or silicon), some microalgae accumulate neutral lipids which, after physical extraction, could be converted, via transesterification with methanol, to biodiesel. Nitrogen-limitation does not appear to increase either cellular lipid content or lipid productivity in Dunaliella. Results from life cycle energy analyses indicate that cultivation of microalgal biomass in open raceway ponds has a positive energy output ratio (EOR), approaching up to 10 (i.e., the caloric energy output from the algae is 10 times greater than the fossil energy inputs), but EOR are less than 1 for biomass grown in engineered photobioreactors. Thus, from both an energetic as well as economic perspective, only open ponds systems can be considered. Significant long-term R&D will be required to make microalgal biofuels processes economically competitive. Specifically, future research should focus on (a) the improvement of biomass productivities (i.e., maximizing solar conversion efficiencies), (b) the selection and isolation of algal strains that can be mass cultured and maintained stably for long periods, (c) the production of algal biomass with a high content of lipids, carbohydrates, and co-products, at high productivity, (d) the low cost harvesting of the biomass, and (e) the extraction and conversion processes to actually derive the biofuels. For Dunaliella specifically, the highest potential is in the co-production of biofuels with high-value animal feeds based on their carotenoid content.

  9. Carbonaceous Aerosols and Radiative Effects Study (CARES), g1-aircraft, sedlacek sp2

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Sedlacek, Art

    The primary objective of the Carbonaceous Aerosol and Radiative Effects Study (CARES) in 2010 was to investigate the evolution of carbonaceous aerosols of different types and their optical and hygroscopic properties in central California, with a focus on the Sacramento urban plume.

  10. DOE ARM SP2 Deployment at Boston College_Aerodyne led Coated...

    Office of Scientific and Technical Information (OSTI)

    ... UC Davis: Chris Cappa and Taylor Helgestad Instrumentation: Photo-acoustic Spectrometer ... Onasch, Andrew Freedman, Leah Williams, Taylor Helgestad, Christopher Cappa, Al Fischer, ...