National Library of Energy BETA

Sample records for tsca toxic substances

  1. Toxic Substances Control Act (TSCA) Polychlorinated Biphenyl (PCB)/Radioactive Waste Annual Inventory for Calendar Year 2014

    SciTech Connect (OSTI)

    Layton, Deborah L.

    2015-06-01

    The Toxic Substances Control Act, 40 CFR 761.65(a)(1) provides an exemption from the one year storage time limit for PCB/radioactive waste. PCB/radioactive waste may exceed the one year time limit provided that the provisions at 40 CFR 761.65(a)(2)(ii) and 40 CFR 761.65(a)(2)(iii) are followed. These two subsections require, (ii) "A written record documenting all continuing attempts to secure disposal is maintained until the waste is disposed of" and (iii) "The written record required by subsection (ii) of this section is available for inspection or submission if requested by EPA." EPA Region 10 has requested the Department of Energy (DOE) to submit an inventory of radioactive-contaminated PCB waste in storage at the Idaho National Laboratory (INL) for the previous calendar year. The annual inventory is separated into two parts, INL without Advanced Mixed Waste Treatment Project (AMWTP) (this includes Battelle Energy Alliance, LLC, CH2M-WG Idaho, LLC, and the Naval Reactors Facility), and AMWTP.

  2. Toxic Substances Control Act (TSCA) Polychlorinated Biphenyl (PCB)/Radioactive Waste Annual Inventory for Calendar Year 2013

    SciTech Connect (OSTI)

    no author on report

    2014-06-01

    The Toxic Substances Control Act, 40 CFR 761.65(a)(1) provides an exemption from the one year storage time limit for PCB/radioactive waste. PCB/radioactive waste may exceed the one year time limit provided that the provisions at 40 CFR 761.65(a)(2)(ii) and 40 CFR 761.65(a)(2)(iii) are followed. These two subsections require, (ii) "A written record documenting all continuing attempts to secure disposal is maintained until the waste is disposed of" and (iii) "The written record required by subsection (ii) of this section is available for inspection or submission if requested by EPA." EPA Region 10 has requested the Department of Energy (DOE) to submit an inventory of radioactive-contaminated PCB waste in storage at the Idaho National Laboratory (INL) for the previous calendar year. The annual inventory is separated into two parts, INL without Advanced Mixed Waste Treatment Project (AMWTP) (this includes Battelle Energy Alliance, LLC, CH2M-WG Idaho, LLC, and the Naval Reactors Facility), and AMWTP.

  3. Toxic Substances Control Act Uranium Enrichment Federal Facility Compliance

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Agreement | Department of Energy Toxic Substances Control Act Uranium Enrichment Federal Facility Compliance Agreement Toxic Substances Control Act Uranium Enrichment Federal Facility Compliance Agreement Toxic Substances Control Act (TSCA) Uranium Enrichment Federal Facility Compliance Agreement establishes a plan to bring DOE's Uranium Enrichment Plants (and support facilities) located in Portsmouth, Ohio and Paducah, Kentucky and DOE's former Uranium Enrichment Plant (and support

  4. Toxic Substances Control Act Uranium Enrichment Federal Facilities Compliance Agreement, February 20, 1992 Summary

    Office of Environmental Management (EM)

    Toxic Substance Control Act Uranium Enrichment Federal Facilities Compliance Agreement (TSCA-UE- FFCA), February 20, 1992 State Kentucky Agreement Type Compliance Agreement Legal Driver(s) TSCA Scope Summary Establishes responsibilities and commitments for bringing DOE's former and active Uranium Enrichment Plants in Paducah, Portsmouth, and Oak Ridge into compliance with TSCA and PCB Regulations Parties DOE; U.S. EPA Date 2/20/1992 SCOPE * Establish a plan and the responsibilities and

  5. Toxic Substances Control Act Uranium Enrichment Federal Facilities Compliance Agreement, February 20, 1992 Summary

    Office of Environmental Management (EM)

    Toxic Substance Control Act Uranium Enrichment Federal Facilities Compliance Agreement (TSCA-UE- FFCA), February 20, 1992 State Ohio Agreement Type Compliance Agreement Legal Driver(s) TSCA Scope Summary Establishes responsibilities and commitments for bringing DOE's former and active Uranium Enrichment Plants in Paducah, Portsmouth, and Oak Ridge into compliance with TSCA and PCB Regulations Parties DOE; U.S. EPA Date 2/20/1992 SCOPE * Establish a plan and the responsibilities and commitments

  6. Toxic Substances Control Act

    SciTech Connect (OSTI)

    Not Available

    1992-05-15

    This Reference Book contains a current copy of the Toxic Substances Control Act and those regulations that implement the statute and appear to be most relevant to DOE activities. The document is provided to DOE and contractor staff for informational purposes only and should not be interpreted as legal guidance. Questions concerning this Reference Book may be directed to Mark Petts, EH-231 (202/586-2609).

  7. Toxic Substances Control Act Uranium Enrichment Federal Facility...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Toxic Substances Control Act Uranium Enrichment Federal Facility Compliance Agreement Toxic Substances Control Act Uranium Enrichment Federal Facility Compliance Agreement Toxic...

  8. Pollution prevention opportunity assessment for the K-25 Site Toxic Substances Control Act Incinerator Operations, Level III

    SciTech Connect (OSTI)

    1995-09-01

    A Level III pollution prevention opportunity assessment (PPOA) was performed for the Oak Ridge K-25 Site Toxic Substances Control Act (TSCA) Incinerator to evaluate pollution prevention (P2) options for various waste streams: The main objective of this study was to identify and evaluate options to reduce the quantities of each waste stream generated by the TSCA Incinerator operations to realize significant environmental and/or economic benefits from P2. For each of the waste streams, P2 options were evaluated following the US Environmental Protection Agency (EPA) hierarchy to (1) reduce the quantity of waste generated, (2) recycle the waste, and/or (3) use alternate waste treatment or segregation methods. This report provides process descriptions, identification and evaluation of P2 options, and final recommendations.

  9. Court decision dropping toxic substance rules stands

    SciTech Connect (OSTI)

    Bryant, C.R.

    1993-06-01

    In a somewhat surprising move, the U.S. Department of Labor has decided not to appeal a court decision essentially dropping regulations established by the Occupational Safety and Health Administration (OSHA) for about 400 hazardous substances. The decision leaves unregulated or subject to reduced standards substances that range from carbon monoxide to perchloroethylene. The Labor Department had until March 22, 1993, to appeal the court decision. On July 8, 1992, the U.S. Court of Appeals for the 11th Circuit overturned OSHA's final Air Contaminants Standard, which was promulgated in 1989. The standard established permissible exposure limits (PELs) for 428 toxic substances. In AFL-CIO vs. OSHA, the Court ruled that OSHA failed to make a separate scientific case for evaluating health risks of each chemical. Because of the decision not to appeal, PELs for more than half of the substances regulated by OSHA now are removed from the books or revert to the voluntary industry standards adopted by OSHA in 1970 and in force prior to the 1989 final rule.

  10. Toxic Substances Control Act Uranium Enrichment Federal Facilities...

    Office of Environmental Management (EM)

    of Pesticides and Toxic Substances U.S. Environmental Protection Agency William H. Young Assistant Secretary for Nuclear Energy U.S. Department of Energy Leo P. Duffy...

  11. Glossary of CERCLA, RCRA and TSCA related terms and acronyms. Environmental Guidance

    SciTech Connect (OSTI)

    Not Available

    1993-10-01

    This glossary contains CERCLA, RCRA and TSCA related terms that are most often encountered in the US Department of Energy (DOE) Environmental Restoration and Emergency Preparedness activities. Detailed definitions are included for key terms. The CERCLA definitions included in this glossary are taken from the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA), as amended and related federal rulemakings. The RCRA definitions included in this glossary are taken from the Resource Conservation and Recovery Act (RCRA) and related federal rulemakings. The TSCA definitions included in this glossary are taken from the Toxic Substances and Control Act (TSCA) and related federal rulemakings. Definitions related to TSCA are limited to those sections in the statute and regulations concerning PCBs and asbestos.Other sources for definitions include additional federal rulemakings, assorted guidance documents prepared by the US Environmental Protection Agency (EPA), guidance and informational documents prepared by the US Department of Energy (DOE), and DOE Orders. The source of each term is noted beside the term. Terms presented in this document reflect revised and new definitions published before July 1, 1993.

  12. Acute aquatic toxicity of biodiesel fuels

    SciTech Connect (OSTI)

    Wright, B.; Haws, R.; Little, D.; Reese, D.; Peterson, C.; Moeller, G.

    1995-12-31

    This study develops data on the acute aquatic toxicity of selected biodiesel fuels which may become subject to environmental effects test regulations under the US Toxic Substances Control Act (TSCA). The test substances are Rape Methyl Ester (RME), Rape Ethyl Ester (REE), Methyl Soyate (MS), a biodiesel mixture of 20% REE and 80% Diesel, a biodiesel mixture of 50% REE and diesel, and a reference substance of Phillips D-2 Reference Diesel. The test procedure follows the Daphnid Acute Toxicity Test outlined in 40 CFR {section} 797.1300 of the TSCA regulations. Daphnia Magna are exposed to the test substance in a flow-through system consisting of a mixing chamber, a proportional diluter, and duplicate test chambers. Novel system modifications are described that accommodate the testing of oil-based test substances with Daphnia. The acute aquatic toxicity is estimated by an EC50, an effective concentration producing immobility in 50% of the test specimen.

  13. Mutation assays involving blood cells that metabolize toxic substances

    DOE Patents [OSTI]

    Crespi, Charles L. (Downers Grove, IL); Thilly, William G. (Winchester, MA)

    1985-01-01

    A line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity) is disclosed. Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. Mutation assays using these cells, and other cells with similar characteristics, are also disclosed.

  14. Mutation assays involving blood cells that metabolize toxic substances

    DOE Patents [OSTI]

    Crespi, Charles L. (Marblehead, MA); Thilly, William G. (Winchester, MA)

    1999-01-01

    The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics.

  15. Mutation assays involving blood cells that metabolize toxic substances

    DOE Patents [OSTI]

    Crespi, C.L.; Thilly, W.G.

    1999-08-10

    The present invention pertains to a line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity). Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. The invention also includes mutation assays using these cells, and other cells with similar characteristics. 3 figs.

  16. Structure activity relationships to assess new chemicals under TSCA

    SciTech Connect (OSTI)

    Auletta, A.E.

    1990-12-31

    Under Section 5 of the Toxic Substances Control Act (TSCA), manufacturers must notify the US Environmental Protection Agency (EPA) 90 days before manufacturing, processing, or importing a new chemical substance. This is referred to as a premanufacture notice (PMN). The PMN must contain certain information including chemical identity, production volume, proposed uses, estimates of exposure and release, and any health or environmental test data that are available to the submitter. Because there is no explicit statutory authority that requires testing of new chemicals prior to their entry into the market, most PMNs are submitted with little or no data. As a result, EPA has developed special techniques for hazard assessment of PMN chemicals. These include (1) evaluation of available data on the chemical itself, (2) evaluation of data on analogues of the PMN, or evaluation of data on metabolites or analogues of metabolites of the PMN, (3) use of quantitative structure activity relationships (QSARs), and (4) knowledge and judgement of scientific assessors in the interpretation and integration of the information developed in the course of the assessment. This approach to evaluating potential hazards of new chemicals is used to identify those that are most in need of addition review of further testing. It should not be viewed as a replacement for testing. 4 tabs.

  17. Toxic Substances Control Act Uranium Enrichment Federal Facilities Compliance Agreement, February 20, 1992

    Office of Environmental Management (EM)

    Thomas L. McCall, Jr. http://www.em.doe.gov/ffaa/ortsca.html 4/25/2001 Toxic Substances Control Act Uranium Enrichment Federal Facilities Compliance Agree.. Page 12 of 26 Deputy Assistant Administrator for Federal Facilities Enforcement Office of Enforcement U.S. Environmental Protection Agency Michael F. Wood, Director Compliance Division Office of Compliance Monitoring Office of Pesticides and Toxic Substances U.S. Environmental Protection Agency William H. Young Assistant Secretary for

  18. TOXIC SUBSTANCES FROM COAL COMBUSTION-A COMPREHENSIVE ASSESSMENT

    SciTech Connect (OSTI)

    C.L. Senior; F. Huggins; G.P. Huffman; N. Shah; N. Yap; J.O.L. Wendt; W. Seames; M.R. Ames; A.F. Sarofim; S. Swenson; J.S. Lighty; A. Kolker; R. Finkelman; C.A. Palmer; S.J. Mroczkowski; J.J. Helble; R. Mamani-Paco; R. Sterling; G. Dunham; S. Miller

    2001-06-30

    The Clean Air Act Amendments of 1990 identify a number of hazardous air pollutants (HAPs) as candidates for regulation. Should regulations be imposed on HAP emissions from coal-fired power plants, a sound understanding of the fundamental principles controlling the formation and partitioning of toxic species during coal combustion will be needed. With support from the National Energy Technology Laboratory (NETL), the Electric Power Research Institute, and VTT (Finland), Physical Sciences Inc. (PSI) has teamed with researchers from USGS, MIT, the University of Arizona (UA), the University of Kentucky (UK), the University of Connecticut (UC), the University of Utah (UU) and the University of North Dakota Energy and Environmental Research Center (EERC) to develop a broadly applicable emissions model useful to regulators and utility planners. The new Toxics Partitioning Engineering Model (ToPEM) will be applicable to all combustion conditions including new fuels and coal blends, low-NOx combustion systems, and new power generation plants. Development of ToPEM will be based on PSI's existing Engineering Model for Ash Formation (EMAF). The work discussed in this report covers the Phase II program. Five coals were studied (three in Phase I and two new ones in Phase II). In this work UK has used XAFS and Moessbauer spectroscopies to characterize elements in project coals. For coals, the principal use was to supply direct information about certain hazardous and other key elements (iron) to complement the more complete indirect investigation of elemental modes of occurrence being carried out by colleagues at USGS. Iterative selective leaching using ammonium acetate, HCl, HF, and HNO3, used in conjunction with mineral identification/quantification, and microanalysis of individual mineral grains, has allowed USGS to delineate modes of occurrence for 44 elements. The Phase II coals show rank-dependent systematic differences in trace-element modes of occurrence. The work at UU focused on the behavior of trace metals in the combustion zone by studying vaporization from single coal particles. The coals were burned at 1700 K under a series of fuel-rich and oxygen-rich conditions. The data collected in this study will be applied to a model that accounts for the full equilibrium between carbon monoxide and carbon dioxide. The model also considers many other reactions taking place in the combustion zone, and involves the diffusion of gases into the particle and combustion products away from the particle. A comprehensive study has been conducted at UA to investigate the post-combustion partitioning of trace elements during large-scale combustion of pulverized coal combustion. For many coals, there are three distinct particle regions developed by three separate mechanisms: (1) a submicron fume, (2) a micron-sized fragmentation region, and (3) a bulk (>3 {micro}m) fly ash region. The controlling partitioning mechanisms for trace elements may be different in each of the three particle regions. A substantial majority of semi-volatile trace elements (e.g., As, Se, Sb, Cd, Zn, Pb) volatilize during combustion. The most common partitioning mechanism for semi-volatile elements is reaction with active fly ash surface sites. Experiments conducted under this program at UC focused on measuring mercury oxidation under cooling rates representative of the convective section of a coal-fired boiler to determine the extent of homogeneous mercury oxidation under these conditions. In fixed bed studies at EERC, five different test series were planned to evaluate the effects of temperature, mercury concentration, mercury species, stoichiometric ratio of combustion air, and ash source. Ash samples generated at UA and collected from full-scale power plants were evaluated. Extensive work was carried out at UK during this program to develop new methods for identification of mercury species in fly ash and sorbents. We demonstrated the usefulness of XAFS spectroscopy for the speciation of mercury captured on low-temperature sorbents from combustion flue gases and dev

  19. Environmental Guidance Program reference book: Toxic substances control act. Revision 7

    SciTech Connect (OSTI)

    1994-12-01

    This Reference Book contains a current copy of the Toxic Substances Control Act and those regulations that implement the statute and appear to be most relevant to DOE activities. The document is provided to DOE staff for informational purposes only and should not be interpreted as legal guidance. Questions concerning this Reference Book may be directed to Mark Petts, EH-231 (202/586-2609).

  20. Toxic Substances Control Act. Environmental Guidance Program Reference Book: Revision 6

    SciTech Connect (OSTI)

    Not Available

    1992-05-15

    This Reference Book contains a current copy of the Toxic Substances Control Act and those regulations that implement the statute and appear to be most relevant to DOE activities. The document is provided to DOE and contractor staff for informational purposes only and should not be interpreted as legal guidance. Questions concerning this Reference Book may be directed to Mark Petts, EH-231 (202/586-2609).

  1. Canadian Environmental Protection Act, strategic options for the management of toxic substances: Electric power generation (fossil fuel) sector, report of stakeholder consultations

    SciTech Connect (OSTI)

    1997-12-31

    The Electric Power Generation Sector Issue Table was formed to assess the management of toxic substances released from that sector, and more specifically, to develop (where warranted) goals, targets, and effective and efficient options for managing toxic releases in order to reduce potential risks to human health and the environment. This strategic options report sets out the recommendations of Issue Table members for the management of toxic substances. The introduction includes an industry profile and a review of the provincial management of electric power sector strategic options priority (SOP) substances. Chapter 2 discusses what substances are toxic, estimates releases of SOP substances from the sector, and reviews Issue Table approaches to risk assessment. Chapter 3 outlines Issue Table activities. Chapter 4 screens toxic substance management options, with evaluation of options against 13 groups of criteria. Chapter 5 presents toxic substances management proposals made to the Issue Table by the electric power generation industry, environmental groups, and Environment Canada.

  2. Acute aquatic toxicity and biodegradation potential of biodiesel fuels

    SciTech Connect (OSTI)

    Haws, R.A.; Zhang, X.; Marshall, E.A.; Reese, D.L.; Peterson, C.L.; Moeller, G.

    1995-12-31

    Recent studies on the biodegradation potential and aquatic toxicity of biodiesel fuels are reviewed. Biodegradation data were obtained using the shaker flask method observing the appearance of CO{sub 2} and by observing the disappearance of test substance with gas chromatography. Additional BOD{sub 5} and COD data were obtained. The results indicate the ready biodegradability of biodiesel fuels as well as the enhanced co-metabolic biodegradation of biodiesel and petroleum diesel fuel mixtures. The study examined reference diesel, neat soy oil, neat rape oil, and the methyl and ethyl esters of these vegetable oils as well as various fuel blends. Acute toxicity tests on biodiesel fuels and blends were performed using Oncorhynchus mykiss (Rainbow Trout) in a static non-renewal system and in a proportional dilution flow replacement system. The study is intended to develop data on the acute aquatic toxicity of biodiesel fuels and blends under US EPA Good Laboratory Practice Standards. The test procedure is designed from the guidelines outlined in Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms and the Fish Acute Aquatic Toxicity Test guideline used to develop aquatic toxicity data for substances subject to environmental effects test regulations under TSCA. The acute aquatic toxicity is estimated by an LC50, a lethal concentration effecting mortality in 50% of the test population.

  3. California Environmental Protection Agency Department of Toxic...

    Open Energy Info (EERE)

    Department of Toxic Substances Control Jump to: navigation, search Name: California Environmental Protection Agency Department of Toxic Substances Control Place: Sacramento,...

  4. Acquisition News | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Toxic Substances Control Act (TSCA) regulated waste, such as polychlorinated biphenyls (PCBs) and asbestos. May 28, 2015 DOE Awards Contract for Hanford Site 222-S Laboratory...

  5. OREM Press Releases | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    18 years of operation and more than 35 million pounds of waste safely incinerated, the Toxic Substances Control Act (TSCA) Incinerator will shut down operations on December 2....

  6. Update to agency for toxic substances and disease registry 2012 report on assessment of biota exposure to mercury originating from Savannah River Site.

    SciTech Connect (OSTI)

    Kuhne, W.

    2015-08-10

    The purpose of this report is to 1) update previous Savannah River National Laboratory (SRNL) assessment reports (Kvartek et al. 1994 and Halverson et al. 2008) on the fate of mercury in the Savannah River Site (SRS) environment and 2) address comments and recommendations from the review of SRS by the Agency for Toxic Substances and Disease Registry (ATSDR) concerning the evaluation of exposures to contaminants in biota originating from the SRS. The ATSDR reviewed and evaluated data from SRS, South Carolina Department of Health & Environmental Control (SCDHEC) and the Georgia Department of Natural Resources (GDNR) concerning the non-radioactive contaminant mercury. This report will provide a response and update to conclusions and recommendations made by the ATSDR.

  7. The TSCA interagency testing committee`s approaches to screening and scoring chemicals and chemical groups: 1977-1983

    SciTech Connect (OSTI)

    Walker, J.D.

    1990-12-31

    This paper describes the TSCA interagency testing committee`s (ITC) approaches to screening and scoring chemicals and chemical groups between 1977 and 1983. During this time the ITC conducted five scoring exercises to select chemicals and chemical groups for detailed review and to determine which of these chemicals and chemical groups should be added to the TSCA Section 4(e) Priority Testing List. 29 refs., 1 fig., 2 tabs.

  8. K-1435 Wastewater Treatment System for the Toxic Substances Control Act Incinerator Wastewater at the East Tennessee Technology Park, Oak Ridge, TN

    SciTech Connect (OSTI)

    Beck, Ch.A.; Tiepel, E.W.; Swientoniewski, M.D.; Crow, K.R.

    2008-07-01

    This paper will discuss the design and performance of a wastewater treatment system installed to support the operation of a hazardous waste incinerator. The Oak Ridge Toxic Substances Control Act Incinerator (TSCAI), located at the East Tennessee Technology Park (ETTP), is designed and permitted to treat Resource Conservation and Recovery Act (RCRA) wastes including characteristic and listed wastes and polychlorinated biphenyl (PCB)-contaminated mixed waste. The incinerator process generates acidic gases and particulates which consist of salts, metals, and radionuclides. These off-gases from the incinerator are treated with a wet off-gas scrubber system. The recirculated water is continuously purged (blow down), resulting in a wastewater to be treated. Additional water sources are also collected on the site for treatment, including storm water that infiltrates into diked areas and fire water from the incinerator's suppression system. To meet regulatory requirements for discharge, a wastewater treatment system (WWTS) was designed, constructed, and operated to treat these water sources. The WWTS was designed to provide for periodic fluctuation of contaminant concentrations due to various feed streams to the incinerator. Blow down consists of total suspended solids (TSS) and total dissolved solids (TDS), encompassing metals, radionuclide contamination and trace organics. The system design flow rate range is 7.95 to 17 cubic meters per hour (m3/hr) (35 to 75 gallons per minute; gpm). The system is designed with redundancy to minimize time off-line and to reduce impacts to the TSCAI operations. A novel treatment system uses several unit operations, including chemical feed systems, two-stage chemical reaction treatment, micro-filtration, sludge storage and dewatering, neutralization, granular activated carbon, effluent neutralization, and a complete programmable logic controller (PLC) and human-machine interface (HMI) control system. To meet the space requirements and to provide portability of the WWTS to other applications, the system was installed in three, over-the-road semi trailers, and interconnected with piping and power. Trailers were oriented on a small site footprint to facilitate ease of installation. A remote sump pump skid was provided to convey water from two holding sumps adjacent to the treatment process. An accumulation tank and pump were also provided to receive miscellaneous wastewaters for treatment if they meet the waste acceptance criteria. The paper will include details of the technology used in the design, the requirements for compliance, and the initial performance demonstration and jar testing results. The WWTS successfully allowed for highly efficient, high-volume treatment with compliant discharge to off-site surface water. (authors)

  9. K-1435 Wastewater Treatment System for the Toxic Substances Control Act Incinerator Wastewater at the East Tennessee Technology Park, Oak Ridge, TN

    SciTech Connect (OSTI)

    Swientoniewski M.D.

    2008-02-24

    This paper discusses the design and performance of a wastewater treatment system installed to support the operation of a hazardous waste incinerator. The Oak Ridge Toxic Substances Control Act Incinerator (TSCAI), located at the East Tennessee Technology Park (ETTP), is designed and permitted to treat Resource ConservatioN and Recovery Act (RCRA) wastes including characteristic and listed wastes and polychlorinated biphenyl (PCB)-contaminated mixed waste. the incinerator process generates acidic gases and particulates which consist of salts, metals, and radionuclides. These off-gases from the incinerator are treated with a wet off-gas scrubber system. The recirculated water is continuously purged (below down), resulting in a wastewater to be treated. Additional water sources are also collected on the site for treatment, including storm water that infiltrates into diked areas and fire water from the incinerator's suppression system. To meet regulatory requirements for discharge, a wastewater treatment system (WWTS) was designed, constructed, and operated to treat these water sources. The WWTS was designed to provide for periodic fluctuation of contaminant concentrations due to various feed streams to the incinverator. Blow down consists of total suspended solids (TSS) and total dissolved solids (TDS), encompassing metals, radionuclide contamination and trace organics. The system design flow rate range is 35 to 75 gallons per minute (gpm). The system is designed with redundancy to minimize time off-line and to reduce impacts to the TSCAI operations. A novel treatment system uses several unit operations, including chemical feed systems, two-stage chemical reaction treatment, microfiltration, sludge storage and dewatering, neutralization, granular activated carbon, effluent neutralization, and a complete programmable logic controller (PLC) and human-machine interface (HMI) control system. To meet the space requirements and to provide portability of the WWTS to other applications, the system was installed in three, over-the-road semi trailers, and interconnected with piping and power. Trailers were oriented on a small site footprint to facilitate ease of installation. A remote sump pump skid was provided to convey water from two holding sumps adjacent to the treatment process. An accumulation tank and pump were also provided to receive miscellaneous wastewaters for treatment if they meet the waste acceptance criteria. The paper includes details of the technology used in the design, the requirements for compliance, and the initial performance demonstration and jar testing results. The WWTS successfully allowed for highly efficient, high-volume treatment with compliant discharge to off-site surface water.

  10. Toxic remediation

    DOE Patents [OSTI]

    Matthews, Stephen M. (Alamed County, CA); Schonberg, Russell G. (Santa Clara County, CA); Fadness, David R. (Santa Clara County, CA)

    1994-01-01

    What is disclosed is a novel toxic waste remediation system designed to provide on-site destruction of a wide variety of hazardous organic volatile hydrocarbons, including but not limited to halogenated and aromatic hydrocarbons in the vapor phase. This invention utilizes a detoxification plenum and radiation treatment which transforms hazardous organic compounds into non-hazardous substances.

  11. The Oak Ridge Reservation PCB Federal Facilities Compliance Agreement, October 28, 1996 Summary

    Office of Environmental Management (EM)

    Compliance Agreement Between The United States Department of Energy and The United States Environmental Protection Agency Region 4 - Toxic Substances Control Act (Also Known As The Oak Ridge Reservation Polychlorinated Biphenyl Federal Facilities Compliance Agreement (ORR-PCB-FFCA)) State Tennessee Agreement Type Federal Facility Agreement Legal Driver(s) TSCA Scope Summary Bring DOE's Oak Ridge Reservation (ORR) into compliance with TSCA and its implementing regulations at 40 CFR Part 761,

  12. Method of removing and detoxifying a phosphorus-based substance

    DOE Patents [OSTI]

    Vandegrift, G.F.; Steindler, M.J.

    1985-05-21

    A method of removing a phosphorus-based poisonous substance from water contaminated is presented. In addition, the toxicity of the phosphorus-based substance is also subsequently destroyed. A water-immiscible organic solvent is first immobilized on a supported liquid membrane before the contaminated water is contacted with one side of the supported liquid membrane to absorb the phosphorus-based substance in the organic solvent. The other side of the supported liquid membrane is contacted with a hydroxy-affording strong base to react with phosphorus-based solvated species to form a non-toxic product.

  13. Toxic Remediation System And Method

    DOE Patents [OSTI]

    Matthews, Stephen M. (Alameda County, CA); Schonberg, Russell G. (Santa Clara County, CA); Fadness, David R. (Santa Clara County, CA)

    1996-07-23

    What is disclosed is a novel toxic waste remediation system designed to provide on-site destruction of a wide variety of hazardous organic volatile hydrocarbons, including but not limited to halogenated and aromatic hydrocarbons in the vapor phase. This invention utilizes a detoxification plenum and radiation treatment which transforms hazardous organic compounds into non-hazardous substances.

  14. Method of removing and detoxifying a phosphorus-based substance

    DOE Patents [OSTI]

    Vandegrift, George F. (Bolingbrook, IL); Steindler, Martin J. (Park Forest, IL)

    1989-01-01

    A method of removing organic phosphorus-based poisonous substances from water contaminated therewith and of subsequently destroying the toxicity of the substance is disclosed. Initially, a water-immiscible organic is immobilized on a supported liquid membrane. Thereafter, the contaminated water is contacted with one side of the supported liquid membrane to selectively dissolve the phosphorus-based substance in the organic extractant. At the same time, the other side of the supported liquid membrane is contacted with a hydroxy-affording strong base to react the phosphorus-based substance dissolved by the organic extractant with a hydroxy ion. This forms a non-toxic reaction product in the base. The organic extractant can be a water-insoluble trialkyl amine, such as trilauryl amine. The phosphorus-based substance can be phosphoryl or a thiophosphoryl.

  15. Toxic substances from coal combustion -- A comprehensive assessment

    SciTech Connect (OSTI)

    Senior, C.L.; Huggins, F.E.; Huffman, G.P.; Shan, N.; Yap, N.; Wendt, J.O.L.; Seames, W.; Ames, M.R.; Sarofim, A.F.; Swenson, S.; Lighty, J.; Kolker, A.; Finkelman, R.; Palmer, C.; Mroczkowski, S.; Helble, J.; Mamani-Paco, R.; Sterling, R.; Dunham, G.; Miller, S.

    2000-08-17

    The final program review meeting of Phase II was held on June 22 in Salt Lake City. The goals of the meeting were to present work in progress and to identify the remaining critical experiments or analyses, particularly those involving collaboration among various groups. The information presented at the meeting is summarized in this report. Remaining fixed bed, bench-scale experiments at EERC were discussed. There are more ash samples which can be run. Of particular interest are high carbon ash samples to be generated by the University of Arizona this summer and some ash-derived sorbents that EERC has evaluated on a different program. The use of separation techniques (electrostatic or magnetic) was also discussed as a way to understand the active components in the ash with respect to mercury. XAFS analysis of leached and unleached ash samples from the University of Arizona was given a high priority. In order to better understand the fixed bed test results, CCSEM and Moessbauer analyses of those ash samples need to be completed. Utah plans to analyze the ash from the single particle combustion experiments for those major elements not measured by INAA. USGS must still complete mercury analyses on the whole coals and leaching residues. Priorities for further work at the SHRIMP-RG facility include arsenic on ash surfaces and mercury in sulfide minerals. Moessbauer analyses of coal samples from the University of Utah were completed; samples from the top and bottom layers of containers of five different coals showed little oxidation of pyrite in the top relative to the bottom except for Wyodak.

  16. Field Evaluation of MERCEM Mercury Emission Analyzer System at the Oak Ridge TSCA Incinerator East Tennessee Technology Park Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    2000-03-01

    The authors reached the following conclusions: (1) The two-month evaluation of the MERCEM total mercury monitor from Perkin Elmer provided a useful venue in determining the feasibility of using a CEM to measure total mercury in a saturated flue gas. (2) The MERCEM exhibited potential at a mixed waste incinerator to meet requirements proposed in PS12 under conditions of operation with liquid feeds only at stack mercury concentrations in the range of proposed MACT standards. (3) Performance of the MERCEM under conditions of incinerating solid and liquid wastes simultaneously was less reliable than while feeding liquid feeds only for the operating conditions and configuration of the host facility. (4) The permeation tube calibration method used in this test relied on the CEM internal volumetric and time constants to relate back to a concentration, whereas a compressed gas cylinder concentration is totally independent of the analyzer mass flowmeter and flowrates. (5) Mercury concentration in the compressed gas cylinders was fairly stable over a 5-month period. (6) The reliability of available reference materials was not fully demonstrated without further evaluation of their incorporation into routine operating procedures performed by facility personnel. (7) The degree of mercury control occurring in the TSCA Incinerator off-gas cleaning system could not be quantified from the data collected in this study. (8) It was possible to conduct the demonstration at a facility incinerating radioactively contaminated wastes and to release the equipment for later unrestricted use elsewhere. (9) Experience gained by this testing answered additional site-specific and general questions regarding the operation and maintenance of CEMs and their use in compliance monitoring of total mercury emissions from hazardous waste incinerators.

  17. Federal Substance Abuse Testing Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2014-01-30

    The Order establishes the requirements and responsibilities for the DOE Federal Substance Abuse Testing Program which covers drug and alcohol testing. Supersedes DOE O 3792.3 Chg 1.

  18. Nanoparticle toxicity testing

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nanoparticle toxicity testing 1663 Los Alamos science and technology magazine Latest Issue:October 2015 past issues All Issues » submit Nanoparticle toxicity testing Assessing the potential health hazards of nanotechnology March 25, 2013 Robot In the search for more accurate and efficient techniques to evaluate the health hazards of nanoparticles, Los Alamos researchers are developing artificial human tissues and organs to replace animal test subjects. A new approach to toxicity testing under

  19. Audit Report: IG-0588 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8 Audit Report: IG-0588 March 3, 2003 Treatment of Mixed Incinerable Waste We found the Department of Energy (Department) was not treating its mixed incinerable solid waste expeditiously or cost-effectively. Specifically, the Department: * Did not fully utilize the Toxic Substances Control Act (TSCA) Incinerator or the Vacuum-Assisted Thermal Desorption (VTD) System; * Stored about 2.5 million pounds of incinerable waste at seven sites rather than treating the waste in Oak Ridge; and, * Approved

  20. C:\Program Files\Microsoft Publisher\ig0451.PDF

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    OAK RIDGE RESERVATION DOE/IG-0451 U.S. DEPARTMENT OF ENERGY OFFICE OF INSPECTOR GENERAL OFFICE OF AUDIT SERVICES AUGUST 1999 AUDIT REPORT August 13, 1999 MEMORANDUM FOR THE SECRETARY FROM: Gregory H. Friedman (Signed) Inspector General SUBJECT: INFORMATION: Audit Report on "Waste Incineration at the Oak Ridge Reservation" BACKGROUND The Toxic Substances Control Act (TSCA) Incinerator is located at the East Tennessee Technology Park (ETTP) in Oak Ridge, Tennessee. It is the only

  1. Substance Abuse Testing Program | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Substance Abuse Testing Program Substance Abuse Testing Program Executive Order 12564, Drug-Free Federal Workplace, states in part that "the use of illegal drugs or the use of legal drugs illegally by Federal employees impairs the efficiency of Federal Agencies, jeopardizes public health and safety, and violates the public trust." Substance abuse increases the burden on other employees who do not use illegal drugs and also is an economic problem due to lost productivity in the

  2. Methods of contacting substances and microsystem contactors

    DOE Patents [OSTI]

    TeGrotenhuis, Ward E [Kennewick, WA; Rassat, Scot D [Benton City, WA; Stenkamp, Victoria S [Richland, WA

    2005-05-22

    A microchannel contactor and methods of contacting substances in microchannel apparatus are described. Some preferred embodiments are combined with microchannel heat exchange.

  3. Potential applications of artificial intelligence in computer-based management systems for mixed waste incinerator facility operation

    SciTech Connect (OSTI)

    Rivera, A.L.; Singh, S.P.N.; Ferrada, J.J.

    1991-01-01

    The Department of Energy/Oak Ridge Field Office (DOE/OR) operates a mixed waste incinerator facility at the Oak Ridge K-25 Site, designed for the thermal treatment of incinerable liquid, sludge, and solid waste regulated under the Toxic Substances Control Act (TSCA) and the Resource Conversion and Recovery Act (RCRA). Operation of the TSCA Incinerator is highly constrained as a result of the regulatory, institutional, technical, and resource availability requirements. This presents an opportunity for applying computer technology as a technical resource for mixed waste incinerator operation to facilitate promoting and sustaining a continuous performance improvement process while demonstrating compliance. This paper describes mixed waste incinerator facility performance-oriented tasks that could be assisted by Artificial Intelligence (AI) and the requirements for AI tools that would implement these algorithms in a computer-based system. 4 figs., 1 tab.

  4. Guidance on the management of polychlorinated biphenyls (PCBs)

    SciTech Connect (OSTI)

    Not Available

    1993-11-01

    Polychlorinated biphenyls (PCBs) are a class of synthetic organic chemicals including 209 known isomers, each with from 1 to 10 chlorine atoms on a biphenyl ring. PCBs have a number of desirable properties for industrial applications including thermal stability, flame retardance, and low vapor pressure. Because of these properties, PCBs were widely used as dielectric fluid in electrical equipment such as utility transformers and capacitors. PCBs were also extensively used in hydraulic fluid and heat transfer fluid, in gaskets, as additives in cutting oils and lubricant, and in a variety of other uses. The Toxic Substances Control Act (TSCA) banned the manufacture of PCBs after 1978 in response to emerging information about the adverse health effects of PCBs and their persistence in the environment. In addition, TSCA directed the Administrator of the Environmental Protection Agency (EPA) to prescribe methods for disposal of PCBS, require marking of PCBs with warning labels, and control their use. The TSCA regulations allow continued use of PCBs provided that the use is totally enclosed and does not pose a risk to human health or the environment. However, at the end of their useful life, all PCB materials must be disposed of according to the TSCA regulations. This guidance document uses graphics and flow charts where possible to present the TSCA regulations according to management activities such as use, storage, disposal, and spill cleanup. The document is designed to be read on an as-needed basis; that is, each chapter can stand alone or may be read in combination with others to help the reader determine the regulations relevant to his or her individual situation and needs. Every attempt has been made to include the requirements of other statutes and regulations that apply to PCB materials and provide references for the reader to consult for additional information.

  5. Analyzing health risks due to trace substance emissions from utility fossil-fired plants. Final report

    SciTech Connect (OSTI)

    1997-12-01

    EPRI has undertaken a number of project addressing a range of issues on the potential health effects due to long-term inhalation exposure to trace substances emitted from utility stacks. This report describes particular studies conducted to assess the health risks due to emissions from groups of power plants operated by individual utility companies. Researchers conducted specialized risk assessments for each of the power plants involved by incorporating utility-specific data into a modeling framework developed as part of EPRI`s Comprehensive Risk Evaluation (CORE) project to tailor the analysis for the individual utility. The results indicated the value of using more up-to-date, precise data in conducting risk assessments, rather than default assumptions. The report also describes CRAFT, the Comprehensive Risk Assessment Framework for Toxics software package, developed to perform these utility-wide air toxics risk assessments.

  6. The possibility of garbage, medical and other toxic waste treatment by plasma chemical method

    SciTech Connect (OSTI)

    Rutberg, P.G.; Safronov, A.A.; Bratsev, A.N.; Kuznetsov, V.E.

    1998-12-31

    This paper describes the creation of plasma facility for treatment of toxic waste. All industrialized countries are greatly interested in plasma chemical technology application for the destruction of different types of industrial, building, purification works toxic waste and waste of plants for garbage treatment. On the basis of three-phase plasma generators with power 0.1--1 MW intended for work in air a row of pilot facilities were created for carrying out of experiments on destruction of medical waste and fluorine-chlorine containing substances. The obtained results allow to design and create pilot-commercial plants with treatment productivity of 200 t/24 hours.

  7. Toxicity of Uranium Adsorbent Materials using the Microtox Toxicity Test

    SciTech Connect (OSTI)

    Park, Jiyeon; Jeters, Robert T.; Gill, Gary A.; Kuo, Li-Jung; Bonheyo, George T.

    2015-10-01

    The Marine Sciences Laboratory at the Pacific Northwest National Laboratory evaluated the toxicity of a diverse range of natural and synthetic materials used to extract uranium from seawater. The uranium adsorbent materials are being developed as part of the U. S. Department of Energy, Office of Nuclear Energy, Fuel Resources Program. The goal of this effort was to identify whether deployment of a farm of these materials into the marine environment would have any toxic effects on marine organisms.

  8. Incinerator Completes Mission in Oak Ridge | Department of Energy

    Energy Savers [EERE]

    Incinerator Completes Mission in Oak Ridge Incinerator Completes Mission in Oak Ridge December 1, 2009 - 12:00pm Addthis OAK RIDGE, Tenn. - After more than 18 years of operation and more than 35 million pounds of waste safely incinerated, the U.S. Department of Energy's (DOE) Toxic Substances Control Act (TSCA) Incinerator at the East Tennessee Technology Park (former K-25 site) in Oak Ridge shut down operations on December 2. During its operating lifetime, the one-of-a-kind thermal treatment

  9. Title 40 CFR 300 National Oil and Hazardous Substances Pollution...

    Open Energy Info (EERE)

    0 National Oil and Hazardous Substances Pollution Contingency Plan Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- Federal RegulationFederal...

  10. Page 9, Department of Energy Substance Abuse Testing Program

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Executive Order 12564, Drug-Free Federal Workplace, applies to all employees, even if your ... Substance Abuse Testing Program covers all Federal employees of the Department of Energy. ...

  11. Toxicity evaluation and hazard review Cold Smoke

    SciTech Connect (OSTI)

    Archuleta, M.M.; Stocum, W.E.

    1993-12-01

    Cold Smoke is a dense white smoke produced by the reaction of titanium tetrachloride and aqueous ammonia aerosols. Early studies on the toxicity of this nonpyrotechnically generated smoke indicated that the smoke itself is essentially non-toxic (i.e. exhibits to systemic toxicity or organ damage due to exposure) under normal deployment conditions. The purpose of this evaluation was to review and summarize the recent literature data available on the toxicity of Cold Smoke, its chemical constituents, and its starting materials.

  12. Process and apparatus for detecting presence of plant substances

    DOE Patents [OSTI]

    Kirby, John A.

    1991-01-01

    An apparatus and process for detecting the presence of plant substances in a particular environment which comprises the steps of: measuring the background K40 gamma ray radiation level in a particular environment with a 1.46 MeV gamma ray counter system; measuring the amount of K40 gamma ray radiation emanating from a package containing a plant substance being passed through an environment with a counter; and generating an alarm signal when the total K40 gamma ray radiation reaches a predetermined level over and above the background level.

  13. Enhanced toxic cloud knockdown spray system for decontamination applications

    DOE Patents [OSTI]

    Betty, Rita G. (Rio Rancho, NM); Tucker, Mark D. (Albuquerque, NM); Brockmann, John E. (Albuquerque, NM); Lucero, Daniel A. (Albuquerque, NM); Levin, Bruce L. (Tijeras, NM); Leonard, Jonathan (Albuquerque, NM)

    2011-09-06

    Methods and systems for knockdown and neutralization of toxic clouds of aerosolized chemical or biological warfare (CBW) agents and toxic industrial chemicals using a non-toxic, non-corrosive aqueous decontamination formulation.

  14. Molecular structure in soil humic substances: The new view

    SciTech Connect (OSTI)

    Sutton, Rebecca; Sposito, Garrison

    2005-04-21

    A critical examination of published data obtained primarily from recent nuclear magnetic resonance spectroscopy, X-ray absorption near-edge structure spectroscopy, electrospray ionization-mass spectrometry, and pyrolysis studies reveals an evolving new view of the molecular structure of soil humic substances. According to the new view, humic substances are collections of diverse, relatively low molecular mass components forming dynamic associations stabilized by hydrophobic interactions and hydrogen bonds. These associations are capable of organizing into micellar structures in suitable aqueous environments. Humic components display contrasting molecular motional behavior and may be spatially segregated on a scale of nanometers. Within this new structural context, these components comprise any molecules intimately associated with a humic substance, such that they cannot be separated effectively by chemical or physical methods. Thus biomolecules strongly bound within humic fractions are by definition humic components, a conclusion that necessarily calls into question key biogeochemical pathways traditionally thought to be required for the formation of humic substances. Further research is needed to elucidate the intermolecular interactions that link humic components into supramolecular associations and to establish the pathways by which these associations emerge from the degradation of organic litter.

  15. Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers Breakout Session 1-D: The Pitch Renewable,...

  16. Relationship Between Composition and Toxicity of Engine Emission...

    Broader source: Energy.gov (indexed) [DOE]

    More Documents & Publications Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed Natural Gas (CNG)-Powered Vehicles Comparative Toxicity of Combined Particle ...

  17. Assessing the toxic effects of ethylene glycol ethers using Quantitative Structure Toxicity Relationship models

    SciTech Connect (OSTI)

    Ruiz, Patricia; Mumtaz, Moiz; Gombar, Vijay

    2011-07-15

    Experimental determination of toxicity profiles consumes a great deal of time, money, and other resources. Consequently, businesses, societies, and regulators strive for reliable alternatives such as Quantitative Structure Toxicity Relationship (QSTR) models to fill gaps in toxicity profiles of compounds of concern to human health. The use of glycol ethers and their health effects have recently attracted the attention of international organizations such as the World Health Organization (WHO). The board members of Concise International Chemical Assessment Documents (CICAD) recently identified inadequate testing as well as gaps in toxicity profiles of ethylene glycol mono-n-alkyl ethers (EGEs). The CICAD board requested the ATSDR Computational Toxicology and Methods Development Laboratory to conduct QSTR assessments of certain specific toxicity endpoints for these chemicals. In order to evaluate the potential health effects of EGEs, CICAD proposed a critical QSTR analysis of the mutagenicity, carcinogenicity, and developmental effects of EGEs and other selected chemicals. We report here results of the application of QSTRs to assess rodent carcinogenicity, mutagenicity, and developmental toxicity of four EGEs: 2-methoxyethanol, 2-ethoxyethanol, 2-propoxyethanol, and 2-butoxyethanol and their metabolites. Neither mutagenicity nor carcinogenicity is indicated for the parent compounds, but these compounds are predicted to be developmental toxicants. The predicted toxicity effects were subjected to reverse QSTR (rQSTR) analysis to identify structural attributes that may be the main drivers of the developmental toxicity potential of these compounds.

  18. Method for warning of radiological and chemical substances using detection paints on a vehicle surface

    DOE Patents [OSTI]

    Farmer, Joseph C. (Tracy, CA)

    2012-03-13

    A system for warning of corrosion, chemical, or radiological substances. The system comprises painting a surface with a paint or coating that includes an indicator material and monitoring the surface for indications of the corrosion, chemical, or radiological substances.

  19. Production of Giant magnetocaloric Substances Using Commercially Available

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Raw Materials - Energy Innovation Portal Building Energy Efficiency Building Energy Efficiency Advanced Materials Advanced Materials Find More Like This Return to Search Production of Giant magnetocaloric Substances Using Commercially Available Raw Materials Ames Laboratory Contact AMES About This Technology Technology Marketing SummaryThe giant magnetocaloric material Gd5(SixGe1-x)4, useful for various types of refrigeration applications, from liquifaction of helium (4K) to room temperature

  20. Forceful Fluid: Scientists Discover a Starchy Substance with Oily Applications

    Broader source: Energy.gov [DOE]

    Researchers at the Energy Department’s Lawrence Livermore National Laboratory (LLNL) set out to find the proper mix of fluids needed to cap the powerful flow of oil that can occur during a spill, an objective that was principally driven by the failure of the top-kill method during last year's oil spill in the Gulf of Mexico. You'll be surprised what starchy substance made the grade.

  1. Paths and ionization losses of proton energy in different substances

    SciTech Connect (OSTI)

    Vasilovskiy, I.M.; Karpov, I.I.; Petrukhin, V.I.; Prokoshkin, Yu.D.

    1986-02-14

    Ionization energy losses of charged particles in a substance are described by the well-known Bethe-Bloch formula. However, the magnitudes of the ionization potentials in region of low proton energies (E < 100 MeV) for heavy elements prove to be considerably larger than those at high energies. Thus, studies of ionization losses in the region of high energies are the main source of the experimental information necessary for the correction of the Bethe-Bloch formula and determination of magnitudes of ionization potentials I. The purpose of this work was to measure the magnitudes of ionization losses dE/ds, paths R and ionization potentials I at a proton energy of E 670 MeV. The measurements were taken by the relative method for different substances of x, and the magnitudes of q sub x=(dE/ds) sub x/(dE/ds) sub Al and px=R sub x/R sub Al were found. Quantities qx and px weakly depend on the energy E where at E=200-600 MeV, a=(2-4).10-2 for different substances. The proton energy was determined with an accuracy of 2 MeV.

  2. Ozone-depleting-substance control and phase-out plan

    SciTech Connect (OSTI)

    Nickels, J.M.; Brown, M.J.

    1994-07-01

    Title VI of the Federal Clean Air Act Amendments of 1990 requires regulation of the use and disposal of ozone-depleting substances (ODSs) (e.g., Halon, Freon). Several important federal regulations have been promulgated that affect the use of such substances at the Hanford Site. On April 23, 1993, Executive Order (EO) 12843, Procurement Requirements and Policies for Federal Agencies for Ozone-Depleting Substances (EPA 1993) was issued for Federal facilities to conform to the new US Environmental Protection Agency (EPA) regulations implementing the Clean Air Act of 1963 (CAA), Section 613, as amended. To implement the requirements of Title VI the US Department of Energy, Richland Operations Office (RL), issued a directive to the Hanford Site contractors on May 25, 1994 (Wisness 1994). The directive assigns Westinghouse Hanford Company (WHC) the lead in coordinating the development of a sitewide comprehensive implementation plan to be drafted by July 29, 1994 and completed by September 30, 1994. The implementation plan will address several areas where immediate compliance action is required. It will identify all current uses of ODSs and inventories, document the remaining useful life of equipment that contains ODS chemicals, provide a phase-out schedule, and provide a strategy that will be implemented consistently by all the Hanford Site contractors. This plan also addresses the critical and required elements of Federal regulations, the EO, and US Department of Energy (DOE) guidance. This plan is intended to establish a sitewide management system to address the clean air requirements.

  3. Hazard index for underground toxic material

    SciTech Connect (OSTI)

    Smith, C.F.; Cohen, J.J.; McKone, T.E.

    1980-06-01

    To adequately define the problem of waste management, quantitative measures of hazard must be used. This study reviews past work in the area of hazard indices and proposes a geotoxicity hazard index for use in characterizing the hazard of toxic material buried underground. Factors included in this index are: an intrinsic toxicity factor, formulated as the volume of water required for dilution to public drinking-water levels; a persistence factor to characterize the longevity of the material, ranging from unity for stable materials to smaller values for shorter-lived materials; an availability factor that relates the transport potential for the particular material to a reference value for its naturally occurring analog; and a correction factor to accommodate the buildup of decay progeny, resulting in increased toxicity.

  4. DOE contractor's meeting on chemical toxicity

    SciTech Connect (OSTI)

    Not Available

    1987-01-01

    The Office of Health and Environmental Research (OHER) is required to determine the potential health and environmental effects associated with energy production and use. To ensure appropriate communication among investigators and scientific disciplines that these research studies represent, OHER has sponsored workshops. This document provides a compilation of activities at the Third Annual DOE/OHER Workshop. This year's workshop was broadened to include all OHER activities identified as within the chemical effects area. The workshop consisted of eight sessions entitled Isolation and Detection of Toxic chemicals; Adduct Formation and Repair; Chemical Toxicity (Posters); Metabolism and Genotoxicity; Inhalation Toxicology; Gene Regulation; Metals Toxicity; and Biological Mechanisms. This document contains abstracts of the information presented by session.

  5. Acute inhalation toxicity of carbonyl sulfide

    SciTech Connect (OSTI)

    Benson, J.M.; Hahn, F.F.; Barr, E.B. [and others

    1995-12-01

    Carbonyl sulfide (COS), a colorless gas, is a side product of industrial procedures sure as coal hydrogenation and gasification. It is structurally related to and is a metabolite of carbon disulfide. COS is metabolized in the body by carbonic anhydrase to hydrogen sulfide (H{sub 2}S), which is thought to be responsible for COS toxicity. No threshold limit value for COS has been established. Results of these studies indicate COS (with an LC{sub 50} of 590 ppm) is slightly less acutely toxic than H{sub 2}S (LC{sub 50} of 440 ppm).

  6. Separation of a target substance from a fluid or mixture using encapsulated sorbents

    DOE Patents [OSTI]

    Aines, Roger D; Spadaccini, Christopher M; Stolaroff, Joshuah K; Bourcier, William L; Lewis, Jennifer A; Duoss, Eric B; Vericella, John J

    2014-09-16

    Method and apparatus for separating a target substance from a fluid or mixture. Capsules having a coating and stripping solvents encapsulated in the capsules are provided. The coating is permeable to the target substance. The capsules having a coating and stripping solvents encapsulated in the capsules are exposed to the fluid or mixture. The target substance migrates through the coating and is taken up by the stripping solvents. The target substance is separated from the fluid or mixture by driving off the target substance from the capsules.

  7. Method and apparatus for injecting a substance into the bloodstream of a subject

    DOE Patents [OSTI]

    Lambrecht, Richard M. (Quogue, NY); Bennett, Gerald W. (East Moriches, NY); Duncan, Charles C. (New Haven, CT); Ducote, Louis W. (Shoreham, NY)

    1983-10-18

    An apparatus and method for injecting a substance, such as a radiopharmaceutical, into the bloodstream of a subject. The apparatus comprises an injection means, such as a servo controlled syringe, a means for measuring the concentration of that substance in the subject's bloodstream, and means for controlling the injection in response to the measurement so that the concentration of the substance follows a predetermined function of time. The apparatus of the subject invention functions to inject a substance into a subject's bloodstream at a rate controlled by an error signal proportional to the difference between the concentration of the substance in the subject's bloodstream and the predetermined function.

  8. Method and apparatus for injecting a substance into the bloodstream of a subject

    DOE Patents [OSTI]

    Lambrecht, R.M.; Bennett, G.W.; Duncan, C.C.; Ducote, L.W.

    1983-10-18

    An apparatus and method is disclosed for injecting a substance, such as a radiopharmaceutical, into the bloodstream of a subject. The apparatus comprises an injection means, such as a servo controlled syringe, a means for measuring the concentration of that substance in the subject's bloodstream, and means for controlling the injection in response to the measurement so that the concentration of the substance follows a predetermined function of time. The apparatus of the subject invention functions to inject a substance into a subject's bloodstream at a rate controlled by an error signal proportional to the difference between the concentration of the substance in the subject's bloodstream and the predetermined function. 2 figs.

  9. Reference manual for toxicity and exposure assessment and risk characterization. CERCLA Baseline Risk Assessment

    SciTech Connect (OSTI)

    1995-03-01

    The Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA, 1980) (CERCLA or Superfund) was enacted to provide a program for identifying and responding to releases of hazardous substances into the environment. The Superfund Amendments and Reauthorization Act (SARA, 1986) was enacted to strengthen CERCLA by requiring that site clean-ups be permanent, and that they use treatments that significantly reduce the volume, toxicity, or mobility of hazardous pollutants. The National Oil and Hazardous Substances Pollution Contingency Plan (NCP) (USEPA, 1985; USEPA, 1990) implements the CERCLA statute, presenting a process for (1) identifying and prioritizing sites requiring remediation and (2) assessing the extent of remedial action required at each site. The process includes performing two studies: a Remedial Investigation (RI) to evaluate the nature, extent, and expected consequences of site contamination, and a Feasibility Study (FS) to select an appropriate remedial alternative adequate to reduce such risks to acceptable levels. An integral part of the RI is the evaluation of human health risks posed by hazardous substance releases. This risk evaluation serves a number of purposes within the overall context of the RI/FS process, the most essential of which is to provide an understanding of ``baseline`` risks posed by a given site. Baseline risks are those risks that would exist if no remediation or institutional controls are applied at a site. This document was written to (1) guide risk assessors through the process of interpreting EPA BRA policy and (2) help risk assessors to discuss EPA policy with regulators, decision makers, and stakeholders as it relates to conditions at a particular DOE site.

  10. Automated process for solvent separation of organic/inorganic substance

    DOE Patents [OSTI]

    Schweighardt, F.K.

    1986-07-29

    There is described an automated process for the solvent separation of organic/inorganic substances that operates continuously and unattended and eliminates potential errors resulting from subjectivity and the aging of the sample during analysis. In the process, metered amounts of one or more solvents are passed sequentially through a filter containing the sample under the direction of a microprocessor control apparatus. The mixture in the filter is agitated by ultrasonic cavitation for a timed period and the filtrate is collected. The filtrate of each solvent extraction is collected individually and the residue on the filter element is collected to complete the extraction process. 4 figs.

  11. Automated process for solvent separation of organic/inorganic substance

    DOE Patents [OSTI]

    Schweighardt, Frank K. (Upper Macungie, PA)

    1986-01-01

    There is described an automated process for the solvent separation of organic/inorganic substances that operates continuously and unattended and eliminates potential errors resulting from subjectivity and the aging of the sample during analysis. In the process, metered amounts of one or more solvents are passed sequentially through a filter containing the sample under the direction of a microprocessor control apparatus. The mixture in the filter is agitated by ultrasonic cavitation for a timed period and the filtrate is collected. The filtrate of each solvent extraction is collected individually and the residue on the filter element is collected to complete the extraction process.

  12. Toxicity Data to Determine Refrigerant Concentration Limits

    SciTech Connect (OSTI)

    Calm, James M.

    2000-09-30

    This report reviews toxicity data, identifies sources for them, and presents resulting exposure limits for refrigerants for consideration by qualified parties in developing safety guides, standards, codes, and regulations. It outlines a method to calculate an acute toxicity exposure limit (ATEL) and from it a recommended refrigerant concentration limit (RCL) for emergency exposures. The report focuses on acute toxicity with particular attention to lethality, cardiac sensitization, anesthetic and central nervous system effects, and other escape-impairing effects. It addresses R-11, R-12, R-22, R-23, R-113, R-114, R-116, R-123, R-124, R-125, R-134, R-134a, R-E134, R-141b, R-142b, R-143a, R-152a, R-218, R-227ea, R-236fa, R-245ca, R-245fa, R-290, R-500, R-502, R-600a, R-717, and R-744. It summarizes additional data for R-14, R-115, R-170 (ethane), R-C318, R-600 (n-butane), and R-1270 (propylene) to enable calculation of limits for blends incorporating them. The report summarizes the data a nd related safety information, including classifications and flammability data. It also presents a series of tables with proposed ATEL and RCL concentrations-in dimensionless form and the latter also in both metric (SI) and inch-pound (IP) units of measure-for both the cited refrigerants and 66 zerotropic and azeotropic blends. They include common refrigerants, such as R-404A, R-407C, R-410A, and R-507A, as well as others in commercial or developmental status. Appendices provide profiles for the cited single-compound refrigerants and for R-500 and R-502 as well as narrative toxicity summaries for common refrigerants. The report includes an extensive set of references.

  13. Hydrogen and Gaseous Fuel Safety and Toxicity

    SciTech Connect (OSTI)

    Lee C. Cadwallader; J. Sephen Herring

    2007-06-01

    Non-traditional motor fuels are receiving increased attention and use. This paper examines the safety of three alternative gaseous fuels plus gasoline and the advantages and disadvantages of each. The gaseous fuels are hydrogen, methane (natural gas), and propane. Qualitatively, the overall risks of the four fuels should be close. Gasoline is the most toxic. For small leaks, hydrogen has the highest ignition probability and the gaseous fuels have the highest risk of a burning jet or cloud.

  14. Environmental Compliance at the West Valley Demonstration Project: The Vitrification Permitting Program

    SciTech Connect (OSTI)

    L. C. Salvatori; C. B. Banzer; W. T. Watters

    1996-05-28

    The major environmental laws that apply to the West Valley Demonstration Project (WVDP) are the: Resource Conservation and Recovery Act (RCRA), Clean Air Act (CAA), Clean Water Act (CWA), Safe Drinking Water Act (SDWA), Toxic Substances Control Act (TSCA), National Environmental Policy Act (NEPA), and Emergency Planning and Community Right-To-Know Act (EPCRA). Regulations developed in accordance with these laws are administered by the New York State Department of Environmental Conservation (NYSDEC) and the U.S. Environmental Protection Agency (EPA) through state and federal programs, and regulatory requirements such as permitting. The Environmental Permits & Reports (EP&R) Group of the Environmental Affairs (EA) Department has the primary responsibility for developing a site-wide permitting program for the WVDP and obtaining the necessary permits. This report discusses the permits and the permitting process associated with the Vitrification Facility (VF).

  15. Radiation dose assessment methodology and preliminary dose estimates to support US Department of Energy radiation control criteria for regulated treatment and disposal of hazardous wastes and materials

    SciTech Connect (OSTI)

    Aaberg, R.L.; Baker, D.A.; Rhoads, K.; Jarvis, M.F.; Kennedy, W.E. Jr.

    1995-07-01

    This report provides unit dose to concentration levels that may be used to develop control criteria for radionuclide activity in hazardous waste; if implemented, these criteria would be developed to provide an adequate level of public and worker health protection, for wastes regulated under U.S, Environmental Protection Agency (EPA) requirements (as derived from the Resource Conservation and Recovery Act [RCRA] and/or the Toxic Substances Control Act [TSCA]). Thus, DOE and the US Nuclear Regulatory Commission can fulfill their obligation to protect the public from radiation by ensuring that such wastes are appropriately managed, while simultaneously reducing the current level of dual regulation. In terms of health protection, dual regulation of very small quantities of radionuclides provides no benefit.

  16. Separation of a target substance from a fluid or mixture using...

    Office of Scientific and Technical Information (OSTI)

    the fluid or mixture by driving off the target substance from the capsules. Authors: Aines, Roger D ; Spadaccini, Christopher M ; Stolaroff, Joshuah K ; Bourcier, William L ;...

  17. Yellow phosphorus process to convert toxic chemicals to non-toxic products

    DOE Patents [OSTI]

    Chang, S.G.

    1994-07-26

    The present invention relates to a process for generating reactive species for destroying toxic chemicals. This process first contacts air or oxygen with aqueous emulsions of molten yellow phosphorus. This contact results in rapid production of abundant reactive species such as O, O[sub 3], PO, PO[sub 2], etc. A gaseous or liquid aqueous solution organic or inorganic chemicals is next contacted by these reactive species to reduce the concentration of toxic chemical and result in a non-toxic product. The final oxidation product of yellow phosphorus is phosphoric acid of a quality which can be recovered for commercial use. A process is developed such that the byproduct, phosphoric acid, is obtained without contamination of toxic species in liquids treated. A gas stream containing ozone without contamination of phosphorus containing species is also obtained in a simple and cost-effective manner. This process is demonstrated to be effective for destroying many types of toxic organic, or inorganic, compounds, including polychlorinated biphenyls (PCB), aromatic chlorides, amines, alcohols, acids, nitro aromatics, aliphatic chlorides, polynuclear aromatic compounds (PAH), dyes, pesticides, sulfides, hydroxyamines, ureas, dithionates and the like. 20 figs.

  18. Yellow phosphorus process to convert toxic chemicals to non-toxic products

    DOE Patents [OSTI]

    Chang, Shih-Ger (El Cerrito, CA)

    1994-01-01

    The present invention relates to a process for generating reactive species for destroying toxic chemicals. This process first contacts air or oxygen with aqueous emulsions of molten yellow phosphorus. This contact results in rapid production of abundant reactive species such as O, O.sub.3, PO, PO.sub.2, etc. A gaseous or liquid aqueous solution organic or inorganic chemicals is next contacted by these reactive species to reduce the concentration of toxic chemical and result in a non-toxic product. The final oxidation product of yellow phosphorus is phosphoric acid of a quality which can be recovered for commercial use. A process is developed such that the byproduct, phosphoric acid, is obtained without contamination of toxic species in liquids treated. A gas stream containing ozone without contamination of phosphorus containing species is also obtained in a simple and cost-effective manner. This process is demonstrated to be effective for destroying many types of toxic organic, or inorganic, compounds, including polychlorinated biphenyls (PCB), aromatic chlorides, amines, alcohols, acids, nitro aromatics, aliphatic chlorides, polynuclear aromatic compounds (PAH), dyes, pesticides, sulfides, hydroxyamines, ureas, dithionates and the like.

  19. U.S. Army Toxic and Hazardous

    Office of Legacy Management (LM)

    JUL 2 3 1982 Col. John 0. Spence - U.S. Army Toxic and Hazardous Katerials Agency (DRxTH-AS) Department of the Army Aberdeen Roving Ground, Kd. 21010 .I' r\. Dear Col. Spence: In response to your letter of May 17, 1982. requesting the identification of sites where radiological materials amy have been left or burl& on U.S. Anny Installations, I am enclosing littlngs of military installatlans that may have been involved with the Manhattan Engineer District/Atomic Energy Camnlssion (KED/AEC)

  20. Thin film composition with biological substance and method of making

    SciTech Connect (OSTI)

    Campbell, A.A.; Song, L.

    1999-09-28

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphous structures, organic crystalline structures, and organic amorphous structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobial, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflammatory, steroid, nonsteroid anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor consisting of the compositions listed above.

  1. Thin film composition with biological substance and method of making

    DOE Patents [OSTI]

    Campbell, Allison A. (Kennewick, WA); Song, Lin (Richland, WA)

    1999-01-01

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphus structures, organic crystalline structures, and organic amorphus structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobal, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflamatory, steriod, nonsteriod anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor the compositions listed above.

  2. Relationship Between Composition and Toxicity of Engine Emission Samples |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Emission Samples Relationship Between Composition and Toxicity of Engine Emission Samples 2003 DEER Conference Presentation: Lovelace Respiratory Research Institute PDF icon 2003_deer_mauderly.pdf More Documents & Publications Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed Natural Gas (CNG)-Powered Vehicles Comparative Toxicity of Combined Particle and Semi-Volatile Organic Fractioins of Gasoline and Diesel Emissions Relationship Between

  3. Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers Breakout Session 1-D: The Pitch Renewable, Non-Toxic and Cost Competitive Solvents and Plasticizers Len Rand, Chief Executive Officer, Chairman, xF Technologies PDF icon rand_bioenergy_2015.pdf More Documents & Publications Vehicle Technologies Office Merit Review 2015: Development of Industrially Viable Battery Electrode Coatings

  4. Comparative Toxicity of Combined Particle and Semi-Volatile Organic

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fractioins of Gasoline and Diesel Emissions | Department of Energy Toxicity of Combined Particle and Semi-Volatile Organic Fractioins of Gasoline and Diesel Emissions Comparative Toxicity of Combined Particle and Semi-Volatile Organic Fractioins of Gasoline and Diesel Emissions 2002 DEER Conference Presentation: Lovelace Respiratory Research Institute PDF icon 2002_deer_mauderly.pdf More Documents & Publications Relationship Between Composition and Toxicity of Engine Emission Samples

  5. Comparative Toxicity of Combined Particle and Semi-Volatile Organic...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Components Responsible for the Health Effects of Inhaled Engine Emissions Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed Natural Gas (CNG)-Powered Vehicles

  6. Early Proctoscopy is a Surrogate Endpoint of Late Rectal Toxicity...

    Office of Scientific and Technical Information (OSTI)

    Prostate Cancer Treated With Radiotherapy Citation Details In-Document Search Title: Early Proctoscopy is a Surrogate Endpoint of Late Rectal Toxicity in Prostate Cancer Treated ...

  7. Toxic chemical considerations for tank farm releases

    SciTech Connect (OSTI)

    Van Keuren, J.C.; Davis, J.S., Westinghouse Hanford

    1996-08-01

    This topical report contains technical information used to determine the accident consequences of releases of toxic chemical and gases for the Tank Farm Final Safety Analysis report (FSAR).It does not provide results for specific accident scenarios but does provide information for use in those calculations including chemicals to be considered, chemical concentrations, chemical limits and a method of summing the fractional contributions of each chemical. Tank farm composites evaluated were liquids and solids for double shell tanks, single shell tanks, all solids,all liquids, headspace gases, and 241-C-106 solids. Emergency response planning guidelines (ERPGs) were used as the limits.Where ERPGs were not available for the chemicals of interest, surrogate ERPGs were developed. Revision 2 includes updated sample data, an executive summary, and some editorial revisions.

  8. System and method for preconcentrating, identifying, and quantifying chemical and biological substances

    DOE Patents [OSTI]

    Yu, Conrad M. (Antioch, CA); Koo, Jackson C. (San Ramon, CA)

    2000-01-01

    A system and method for preconcentrating, identifying, and quantifying chemical and biological substances is disclosed. An input valve directs a first volume of a sample gas to a surface acoustic wave (SAW) device. The SAW device preconcentrates and detects a mass of a substance within the sample gas. An output valve receives a second volume of the sample gas containing the preconcentrated substance from the SAW device and directs the second volume to a gas chromatograph (GC). The GC identifies the preconcentrated substance within the sample gas. A shunt valve exhausts a volume of the sample gas equal to the first volume minus the second volume away from the SAW device and the GC. The method of the present invention includes the steps of opening an input valve for passing a first volume of a sample gas to a SAW device; preconcentrating and detecting a mass of a substance within the sample gas using the SAW device; opening an output valve for passing a second volume of the sample gas containing the preconcentrated substance to a gas chromatograph (GC); and then identifying the preconcentrated substance within the sample gas using the GC.

  9. Nanoporous Materials Can Tune the Critical Point of a Pure Substance |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Center for Gas SeparationsRelevant to Clean Energy Technologies | Blandine Jerome Nanoporous Materials Can Tune the Critical Point of a Pure Substance Previous Next List Braun, Efrem; Chen, Joseph J.; Schnell, Sondre K.; Lin, Li-Chiang; Reimer, Jeffrey A.; and Smit, Berend. Nanoporous Materials Can Tune the Critical Point of a Pure Substance. Angew. Chem., Int. Ed., 48, 114349-14352 (2015). DOI: 10.1002/anie.201506865 NanoporousMaterialsCanTune Abstract: Molecular simulations and NMR

  10. Contractor Workplace Substance Abuse Program at DOE Sites (10 CFR 707) |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Contractor Workplace Substance Abuse Program at DOE Sites (10 CFR 707) Contractor Workplace Substance Abuse Program at DOE Sites (10 CFR 707) This rule establishes policies, criteria, and procedures for developing and implementing programs to maintain a workplace free from the use of illegal drugs. It applies to DOE contractors performing work at sites owned or controlled by DOE and to individuals with unescorted access to the control areas of certain DOE reactors.

  11. Sol-gel deposition of buffer layers on biaxially textured metal substances

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Sol-gel deposition of buffer layers on biaxially textured metal substances Citation Details In-Document Search Title: Sol-gel deposition of buffer layers on biaxially textured metal substances A method is disclosed for forming a biaxially textured buffer layer on a biaxially oriented metal substrate by using a sol-gel coating technique followed by pyrolyzing/annealing in a reducing atmosphere. This method is advantageous for providing substrates for depositing

  12. Program to monitor Department of Energy workers exposed to hazardous and radioactive substances

    Energy Savers [EERE]

    50 CHAPTER 42 SUBCHAPTER VI Part C 5 2733 Program to monitor Department of Energy workers exposed to hazardous and radioactive substances (a) In general The Secretary shall establish and carry out a program for the identification and on-going medical evaluation of current and former Department of Energy employees who are subject to significant health risks as a result of the exposure of such employees to hazardous or radioactive substances during such empIoyment. (b) Implementation of program (

  13. Guidelines for Transportation, Handling, and Use of Fast Pyrolysis Bio-Oil. Part 1. Flammability and Toxicity

    SciTech Connect (OSTI)

    Oasmaa, Anja; Kalli, Anssi; Lindfors, Christian; Elliott, Douglas C.; Springer, David L.; Peacocke, Cordner; Chiaramonti, David

    2012-05-04

    An alternative sustainable fuel, biomass-derived fast pyrolysis oil or 'bio-oil', is coming into the market. Fast pyrolysis pilot and demonstration plants for fuel applications producing tonnes of bio-oil are in operation, and commercial plants are under design. There will be increasingly larger amounts of bio-oil transportation on water and by land, leading to a need for specifications and supporting documentation. Bio-oil is different from conventional liquid fuels, and therefore must overcome both technical and marketing hurdles for its acceptability in the fuels market. A comprehensive Material Safety Data Sheet (MSDS) is required, backed with independent testing and certification. In order to standardise bio-oil quality specifications are needed. The first bio-oil burner fuel standard in ASTM (D7544) was approved in 2009. CEN standardisation has been initiated in Europe. In the EU a new chemical regulation system, REACH (Registration, Evaluation and Authorisation of Chemicals) is being applied. Registration under REACH has to be made if bio-oil is produced or imported to the EU. In the USA and Canada, bio-oil has to be filed under TOSCA (US Toxic Substances Control Act). In this paper the state of the art on standardisation is discussed, and new data for the transportation guidelines is presented. The focus is on flammability and toxicity.

  14. Oxidative stress-induced autophagy: Role in pulmonary toxicity

    SciTech Connect (OSTI)

    Malaviya, Rama; Laskin, Jeffrey D.; Laskin, Debra L.

    2014-03-01

    Autophagy is an evolutionarily conserved catabolic process important in regulating the turnover of essential proteins and in elimination of damaged organelles and protein aggregates. Autophagy is observed in the lung in response to oxidative stress generated as a consequence of exposure to environmental toxicants. Whether autophagy plays role in promoting cell survival or cytotoxicity is unclear. In this article recent findings on oxidative stress-induced autophagy in the lung are reviewed; potential mechanisms initiating autophagy are also discussed. A better understanding of autophagy and its role in pulmonary toxicity may lead to the development of new strategies to treat lung injury associated with oxidative stress. - Highlights: Exposure to pulmonary toxicants is associated with oxidative stress. Oxidative stress is known to induce autophagy. Autophagy is upregulated in the lung following exposure to pulmonary toxicants. Autophagy may be protective or pathogenic.

  15. Toxic Chemical Release Inventory reporting ``Qs & As``. Environmental Guidance

    SciTech Connect (OSTI)

    Not Available

    1994-03-01

    This document offers guidance on toxic chemical release inventory reporting, pursuant to Section 313 of the Emergency Planning and Community Right-to-Know Act (EPCRA) at DOE sites.

  16. Multiple Species of Bacteria Convert Elemental Mercury to Toxic...

    Office of Science (SC) Website

    Researchers are studying how bacteria transform mercury into a toxic form in the environment that can accumulate in the food web, posing a threat to wildlife and people. The ...

  17. Risk management of undesirable substances in feed following updated risk assessments

    SciTech Connect (OSTI)

    Verstraete, Frans

    2013-08-01

    Directive 2002/32/EC of 7 May 2002 of the European Parliament and of the Council on undesirable substances in animal feed is the framework for the EU action on undesirable substances in feed. This framework Directive provides: ?that products intended for animal feed may enter for use in the Union from third countries, be put into circulation and/or used in the Union only if they are sound, genuine and of merchantable quality and therefore when correctly used do not represent any danger to human health, animal health or to the environment or could adversely affect livestock production. ?that in order to protect animal and public health and the environment, maximum levels for specific undesirable substances shall be established where necessary. ?for mandatory consultation of a scientific body (EFSA) for all provisions which may have an effect upon public health or animal health or on the environment. ?that products intended for animal feed containing levels of an undesirable substance that exceed the established maximum level may not be mixed for dilution purposes with the same, or other, products intended for animal feed and may not be used for the production of compound feed. Based on the provisions and principles laid down in this framework Directive, maximum levels for a whole range of undesirable substances have been established at EU level. During the discussions in view of the adoption of Directive 2002/32/EC, the European Commission made the commitment to review all existing provisions on undesirable substances on the basis of updated scientific risk assessments. Following requests of the European Commission, the Panel on Contaminants in the Food Chain (CONTAM) from the European Food Safety Authority (EFSA) has completed a series of 30 risk assessments undertaken over the last 5 years on undesirable substances in animal feed reviewing the possible risks for animal and human health due to the presence of these substances in animal feed. EU legislation on undesirable substances has undergone recently several changes to take account of these most recent scientific opinions. Furthermore EFSA has assessed the risks for public and animal health following the unavoidable carry-over of coccidiostats into non target feed. Maximum levels for the unavoidable carry-over have been established for the non-target feed and the food of animal origin from non-target animal species.

  18. The hazardous priority substances in Italy: National rules and environmental quality standard in marine environment

    SciTech Connect (OSTI)

    Maggi, Chiara Onorati, Fulvio Lamberti, Claudia Virno Cicero, Anna Maria

    2008-01-15

    Article number 16 of the Water Framework Directive (Directive 2000/60/EC) lays down the community strategy for establishment of harmonised quality standards for the priority substances and other substances posing a significant risk to the aquatic environment. In order to achieve the protection objectives of the Directive 2000/60/EC, the Italian Ministry of the Environment proposed the quality standards for surface water, sediments and biota related to the priority substances listed in the decision No. 2455/2001/EC of the European Parliament and of the Council of November 20 (2001) [Decision N. 2455/2001/EC of the European Parliament and of the Council of 20 November 2001. The list of priority substances in the field of water policy and amending Directive 2000/60/EC. Official Journal of the European Communities, 15.12.2001, p. 5]. Particularly, for the protection of the marine environment, the proposed Italian rules state that, from 1 January 2021, the concentrations of the hazardous priority substances in Italian marine and lagoon waters must be near the natural background for natural substances, like metals, and near zero for the anthropogenic one. According to Directive 2000/60/EC, the Italian Ministry of Environment issued in 2003 Decree 367 in which has derived 160 Environmental Quality Standard (EQS) for water and 27 Environmental Quality Objective (EQO) for sediment of marine coastal area, lagoons and coastal ponds. Biota quality standards have still to be fixed. The paper illustrates the criteria applied for the definition of the quality standards and some comments are presented.

  19. Sol-gel deposition of buffer layers on biaxially textured metal substances

    Office of Scientific and Technical Information (OSTI)

    (Patent) | SciTech Connect Sol-gel deposition of buffer layers on biaxially textured metal substances Citation Details In-Document Search Title: Sol-gel deposition of buffer layers on biaxially textured metal substances × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional information resources in energy

  20. REGULATORY STRATEGIES TO MINIMIZE GENERATION OF REGULATED WASTES FROM CLEANUP, CONTINUED USE OR DECOMMISSIONING OF NUCLEAR FACILITIES CONTAMINATED WITH POLYCHLORINATED BIPHENYLS (PCBS) - 11198

    SciTech Connect (OSTI)

    Lowry, N.

    2010-11-05

    Disposal costs for liquid PCB radioactive waste are among the highest of any category of regulated waste. The high cost is driven by the fact that disposal options are extremely limited. Toxic Substances Control Act (TSCA) regulations require most liquids with PCBs at concentration of {ge} 50 parts-per-million to be disposed by incineration or equivalent destructive treatment. Disposal fees can be as high as $200 per gallon. This figure does not include packaging and the cost to transport the waste to the disposal facility, or the waste generator's labor costs for managing the waste prior to shipment. Minimizing the generation of liquid radioactive PCB waste is therefore a significant waste management challenge. PCB spill cleanups often generate large volumes of waste. That is because the removal of PCBs typically requires the liberal use of industrial solvents followed by a thorough rinsing process. In a nuclear facility, the cleanup process may be complicated by the presence of radiation and other occupational hazards. Building design and construction features, e.g., the presence of open grating or trenches, may also complicate cleanup. In addition to the technical challenges associated with spill cleanup, selection of the appropriate regulatory requirements and approach may be challenging. The TSCA regulations include three different sections relating to the cleanup of PCB contamination or spills. EPA has also promulgated a separate guidance policy for fresh PCB spills that is published as Subpart G of 40 CFR 761 although it is not an actual regulation. Applicability is based on the circumstances of each contamination event or situation. Other laws or regulations may also apply. Identification of the allowable regulatory options is important. Effective communication with stakeholders, particularly regulators, is just as important. Depending on the regulatory path that is taken, cleanup may necessitate the generation of large quantities of regulated waste. Allowable options must be evaluated carefully in order to reduce compliance risks, protect personnel, limit potential negative impacts on facility operations, and minimize the generation of wastes subject to TSCA. This paper will identify critical factors in selecting the appropriate TSCA regulatory path in order to minimize the generation of radioactive PCB waste and reduce negative impacts to facilities. The importance of communicating pertinent technical issues with facility staff, regulatory personnel, and subsequently, the public, will be discussed. Key points will be illustrated by examples from five former production reactors at the DOE Savannah River Site. In these reactors a polyurethane sealant was used to seal piping penetrations in the biological shield walls. During the intense neutron bombardment that occurred during reactor operation, the sealant broke down into a thick, viscous material that seeped out of the piping penetrations over adjacent equipment and walls. Some of the walls were painted with a PCB product. PCBs from the paint migrated into the degraded sealant, creating PCB 'spill areas' in some of these facilities. The regulatory cleanup approach selected for each facility was based on its operational status, e.g., active, inactive or undergoing decommissioning. The selected strategies served to greatly minimize the generation of radioactive liquid PCB waste. It is expected that this information would be useful to other DOE sites, DOD facilities, and commercial nuclear facilities constructed prior to the 1979 TSCA ban on most manufacturing and uses of PCBs.

  1. New method of determining the current carrier concentration in a substance in the state of superconductivity

    SciTech Connect (OSTI)

    Golotyuk, F.P.

    1985-08-02

    This report proposes a fundamentally new solution to the problem of determining the current carrier concentration in a substance. The basis of the corresponding experimental method is a theory that considers the electron drift energy when considering the oscillatory processes in certain circuits.

  2. Self-filling and self-purging apparatus for detecting spontaneous radiation from substances in fluids

    DOE Patents [OSTI]

    Larson, I. Lauren (Oak Ridge, TN); Chiles, Marion M. (Knoxville, TN); Miller, V. Clint (Concord, TN)

    1993-01-01

    Disclosed herein is a radiation detector providing for the in situ automatic sampling of fluids containing substances emitting radiation, especially Cerenkov radiation. The detector permits sampling within well casings and is self-purging such that no additional provisions must be established for the storage and disposal of contaminated fluids.

  3. Intelligent emissions controller for substance injection in the post-primary combustion zone of fossil-fired boilers

    DOE Patents [OSTI]

    Reifman, Jaques (Western Springs, IL); Feldman, Earl E. (Willowbrook, IL); Wei, Thomas Y. C. (Downers Grove, IL); Glickert, Roger W. (Pittsburgh, PA)

    2003-01-01

    The control of emissions from fossil-fired boilers wherein an injection of substances above the primary combustion zone employs multi-layer feedforward artificial neural networks for modeling static nonlinear relationships between the distribution of injected substances into the upper region of the furnace and the emissions exiting the furnace. Multivariable nonlinear constrained optimization algorithms use the mathematical expressions from the artificial neural networks to provide the optimal substance distribution that minimizes emission levels for a given total substance injection rate. Based upon the optimal operating conditions from the optimization algorithms, the incremental substance cost per unit of emissions reduction, and the open-market price per unit of emissions reduction, the intelligent emissions controller allows for the determination of whether it is more cost-effective to achieve additional increments in emission reduction through the injection of additional substance or through the purchase of emission credits on the open market. This is of particular interest to fossil-fired electrical power plant operators. The intelligent emission controller is particularly adapted for determining the economical control of such pollutants as oxides of nitrogen (NO.sub.x) and carbon monoxide (CO) emitted by fossil-fired boilers by the selective introduction of multiple inputs of substances (such as natural gas, ammonia, oil, water-oil emulsion, coal-water slurry and/or urea, and combinations of these substances) above the primary combustion zone of fossil-fired boilers.

  4. Survey of toxicity and carcinogenity of mineral deposits

    SciTech Connect (OSTI)

    Furst, A.; Harding-Barlow, I.

    1981-11-03

    The toxicities and biogeochemical cycles of arsenic, cadmium, chromium, lead and nickel are reviewed in some detail, and other trace elements briefly mentioned. These heavy metals are used as a framework within which the problem of low-level radioactive waste disposal can be compared. (ACR)

  5. System and method for identifying, reporting, and evaluating presence of substance

    DOE Patents [OSTI]

    Smith, Maurice; Lusby, Michael; Van Hook, Arthur; Cook, Charles J.; Wenski, Edward G.; Solyom, David

    2006-10-24

    A system and method for identifying, reporting, and evaluating a presence of a solid, liquid, gas, or other substance of interest, particularly a dangerous, hazardous, or otherwise threatening chemical, biological, or radioactive substance. The system comprises one or more substantially automated, location self-aware remote sensing units; a control unit; and one or more data processing and storage servers. Data is collected by the remote sensing units and transmitted to the control unit; the control unit generates and uploads a report incorporating the data to the servers; and thereafter the report is available for review by a hierarchy of responsive and evaluative authorities via a wide area network. The evaluative authorities include a group of relevant experts who may be widely or even globally distributed.

  6. System And Method For Identifying, Reporting, And Evaluating Presence Of Substance

    DOE Patents [OSTI]

    Smith, Maurice; Lusby, Michael; Hook, Arthur Van; Cook, Charles J.; Wenski, Edward G.; Solyom, David

    2005-09-20

    A system and method for identifying, reporting, and evaluating a presence of a solid, liquid, gas, or other substance of interest, particularly a dangerous, hazardous, or otherwise threatening chemical, biological, or radioactive substance. The system comprises one or more substantially automated, location self-aware remote sensing units; a control unit; and one or more data processing and storage servers. Data is collected by the remote sensing units and transmitted to the control unit; the control unit generates and uploads a report incorporating the data to the servers; and thereafter the report is available for review by a hierarchy of responsive and evaluative authorities via a wide area network. The evaluative authorities include a group of relevant experts who may be widely or even globally distributed.

  7. System and method for identifying, reporting, and evaluating presence of substance

    DOE Patents [OSTI]

    Smith, Maurice; Lusby, Michael; Van Hook, Arthur; Cook, Charles J.; Wenski, Edward G.; Solyom, David

    2012-02-14

    A system and method for identifying, reporting, and evaluating a presence of a solid, liquid, gas, or other substance of interest, particularly a dangerous, hazardous, or otherwise threatening chemical, biological, or radioactive substance. The system comprises one or more substantially automated, location self-aware remote sensing units; a control unit; and one or more data processing and storage servers. Data is collected by the remote sensing units and transmitted to the control unit; the control unit generates and uploads a report incorporating the data to the servers; and thereafter the report is available for review by a hierarchy of responsive and evaluative authorities via a wide area network. The evaluative authorities include a group of relevant experts who may be widely or even globally distributed.

  8. An assessment of alternatives and technologies for replacing ozone- depleting substances at DOE facilities

    SciTech Connect (OSTI)

    Purcell, C.W.; Miller, K.B.; Friedman, J.R.; Rapoport, R.D.; Conover, D.R.; Hendrickson, P.L.; Koss, T.C.

    1992-10-01

    Title VI of the Clean Air Act, as amended, mandates a production phase-out for ozone-depleting substances (ODSs). These requirements will have a significant impact on US Department of Energy (DOE) facilities. Currently, DOE uses ODSs in three major activities: fire suppression (halon), refrigeration and cooling (chlorofluorocarbons [CFCs]), and cleaning that requires solvents (CFCs, methyl chloroform, and carbon tetrachloride). This report provides basic information on methods and strategies to phase out use of ODSs at DOE facilities.

  9. A study of toxic emissions from a coal-fired power plant utilizing an ESP while demonstrating the ICCT CT-121 FGD Project. Final report

    SciTech Connect (OSTI)

    Not Available

    1994-06-16

    The US Department of Energy is performing comprehensive assessments of toxic emissions from eight selected coal-fired electric utility units. This program responds to the Clean Air Act Amendments of 1990, which require the US Environmental Protection Agency (EPA) to evaluate emissions of hazardous air pollutants (HAPs) from electric utility power plants for Potential health risks. The resulting data will be furnished to EPA utility power plants and health risk determinations. The assessment of emissions involves the collection and analysis of samples from the major input, process, and output streams of each of the eight power plants for selected hazardous Pollutants identified in Title III of the Clean Air Act. Additional goals are to determine the removal efficiencies of pollution control subsystems for these selected pollutants and the Concentrations associated with the particulate fraction of the flue gas stream as a function of particle size. Material balances are being performed for selected pollutants around the entire power plant and several subsystems to identify the fate of hazardous substances in each utility system. Radian Corporation was selected to perform a toxics assessment at a plant demonstrating an Innovative Clean Coal Technology (ICCT) Project. The site selected is Plant Yates Unit No. 1 of Georgia Power Company, which includes a Chiyoda Thoroughbred-121 demonstration project.

  10. Reliability of Quantitative Ultrasonic Assessment of Normal-Tissue Toxicity in Breast Cancer Radiotherapy

    SciTech Connect (OSTI)

    Yoshida, Emi J.; Chen Hao; Torres, Mylin; Andic, Fundagul; Liu Haoyang; Chen Zhengjia; Sun, Xiaoyan; Curran, Walter J.; Liu Tian

    2012-02-01

    Purpose: We have recently reported that ultrasound imaging, together with ultrasound tissue characterization (UTC), can provide quantitative assessment of radiation-induced normal-tissue toxicity. This study's purpose is to evaluate the reliability of our quantitative ultrasound technology in assessing acute and late normal-tissue toxicity in breast cancer radiotherapy. Method and Materials: Our ultrasound technique analyzes radiofrequency echo signals and provides quantitative measures of dermal, hypodermal, and glandular tissue toxicities. To facilitate easy clinical implementation, we further refined this technique by developing a semiautomatic ultrasound-based toxicity assessment tool (UBTAT). Seventy-two ultrasound studies of 26 patients (720 images) were analyzed. Images of 8 patients were evaluated for acute toxicity (<6 months postradiotherapy) and those of 18 patients were evaluated for late toxicity ({>=}6 months postradiotherapy). All patients were treated according to a standard radiotherapy protocol. To assess intraobserver reliability, one observer analyzed 720 images in UBTAT and then repeated the analysis 3 months later. To assess interobserver reliability, three observers (two radiation oncologists and one ultrasound expert) each analyzed 720 images in UBTAT. An intraclass correlation coefficient (ICC) was used to evaluate intra- and interobserver reliability. Ultrasound assessment and clinical evaluation were also compared. Results: Intraobserver ICC was 0.89 for dermal toxicity, 0.74 for hypodermal toxicity, and 0.96 for glandular tissue toxicity. Interobserver ICC was 0.78 for dermal toxicity, 0.74 for hypodermal toxicity, and 0.94 for glandular tissue toxicity. Statistical analysis found significant changes in dermal (p < 0.0001), hypodermal (p = 0.0027), and glandular tissue (p < 0.0001) assessments in the acute toxicity group. Ultrasound measurements correlated with clinical Radiation Therapy Oncology Group (RTOG) toxicity scores of patients in the late toxicity group. Patients with RTOG Grade 1 or 2 had greater ultrasound-assessed toxicity percentage changes than patients with RTOG Grade 0. Conclusion: Early and late radiation-induced effects on normal tissue can be reliably assessed using quantitative ultrasound.

  11. Saltone 2QCY15 TCLP toxicity and UTS results

    SciTech Connect (OSTI)

    Miller, D. H.

    2015-08-01

    A Saltstone Disposal Facility (SDF) waste form was prepared in the Savannah River National Laboratory (SRNL) from a Tank 50H sample and Z-Area premix material for the second quarter of calendar year 2015 (2QCY15). After a 28 day cure, a sample of the SDF waste form was collected, and shipped to a certified laboratory for Toxic Characteristic and Universal Treatment Standards (UTS) analysis. The metals analysis is performed using the Toxic Characteristic Leaching Procedure (TCLP)1. The 2QCY15 saltstone sample results meet South Carolina Hazardous Waste Management Regulations (SCHWMR) R.61-79.261.24 and R.61-79.268.48(a) requirements for a nonhazardous waste form with respect to RCRA metals and underlying hazardous constituents (UHC).

  12. Saltstone 1QCY15 TCLP Toxicity and UTS Results

    SciTech Connect (OSTI)

    Miller, D.

    2015-07-29

    A Saltstone Disposal Facility (SDF) waste form was prepared in the Savannah River National Laboratory (SRNL) from a Tank 50H sample and Z-Area premix material for the first quarter of calendar year 2015 (1QCY15). After a 28 day cure, a sample of the SDF waste form was collected, and shipped to a certified laboratory for Toxic Characteristic and Universal Treatment Standards (UTS) analysis. The metals analysis is performed using the Toxic Characteristic Leaching Procedure (TCLP). The 1QCY15 saltstone sample results meet South Carolina Hazardous Waste Management Regulations (SCHWMR) R.61-79.261.24 and R.61-79.268.48(a) requirements for a nonhazardous waste form with respect to RCRA metals and underlying hazardous constituents (UHC).

  13. Saltstone 2QCY15 TCLP toxicity and UTS results

    SciTech Connect (OSTI)

    Miller, D. H.

    2015-07-31

    A Saltstone Disposal Facility (SDF) waste form was prepared in the Savannah River National Laboratory (SRNL) from a Tank 50H sample and Z-Area premix material for the second quarter of calendar year 2015 (2QCY15). After a 28 day cure, a sample of the SDF waste form was collected, and shipped to a certified laboratory for Toxic Characteristic and Universal Treatment Standards (UTS) analysis. The metals analysis is performed using the Toxic Characteristic Leaching Procedure (TCLP). The 2QCY15 saltstone sample results meet South Carolina Hazardous Waste Management Regulations (SCHWMR) R.61-79.261.24 and R.61-79.268.48(a) requirements for a nonhazardous waste form with respect to RCRA metals and underlying hazardous constituents (UHC).

  14. Saltstone 3QCY15 TCLP Toxicity and UTS Results

    SciTech Connect (OSTI)

    Miller, D.

    2015-12-09

    A Saltstone Disposal Facility (SDF) waste form was prepared in the Savannah River National Laboratory (SRNL) from a Tank 50H sample and Z-Area premix material for the third quarter of calendar year 2015 (3QCY15). After a 28 day cure, a sample of the SDF waste form was collected, and shipped to a certified laboratory for Toxic Characteristic and Universal Treatment Standards (UTS) analysis. The metals analysis is performed using the Toxic Characteristic Leaching Procedure (TCLP).1 The 3QCY15 saltstone sample results meet South Carolina Hazardous Waste Management Regulations (SCHWMR) R.61-79.261.24 and R.61-79.268.48(a) requirements for a nonhazardous waste form with respect to RCRA metals and underlying hazardous constituents (UHC).

  15. Surviving the toxics in south Louisiana: A minority perspective

    SciTech Connect (OSTI)

    Wright, B.

    1995-12-01

    The Louisiana industrial corridor along the Mississippi River is lined with 136 petro-chemical plants and serves oil refineries. This approximates nearly one plant or refinery for every half mile of the river. The air, ground, and water along this corridor are so full or carcinogens that it has been described as a massive human experiment. Poor blacks live in river towns near the brunt of this discharge. Total mortality rates and cancer mortality rates in counties along the Mississippi River are significantly higher than in the rest of the nation`s counties. Moreover, the areas of greatest toxic discharge. Findings of disproportionately high mortality rates along the Mississippi, especially in communities on the lower river where toxic discharge minority and poor communities along the Mississippi River chemical corridor.

  16. Reactive formulations for a neutralization of toxic industrial chemicals

    DOE Patents [OSTI]

    Tucker, Mark D.; Betty, Rita G.

    2006-10-24

    Decontamination formulations for neutralization of toxic industrial chemicals, and methods of making and using same. The formulations are effective for neutralizing malathion, hydrogen cyanide, sodium cyanide, butyl isocyanate, carbon disulfide, phosgene gas, capsaicin in commercial pepper spray, chlorine gas, anhydrous ammonia gas; and may be effective at neutralizing hydrogen sulfide, sulfur dioxide, formaldehyde, ethylene oxide, methyl bromide, boron trichloride, fluorine, tetraethyl pyrophosphate, phosphorous trichloride, arsine, and tungsten hexafluoride.

  17. Low toxicity method of inhibiting sickling of sickle erythrocytes

    DOE Patents [OSTI]

    Packer, Lester; Bymun, Edwin N.

    1977-01-01

    A low toxicity method of inhibiting sickling of sickle erythrocytes which comprises intermixing the erythrocytes with an effective anti-sickling amount of a water-soluble imidoester of the formula RC(=NH)OR' wherein R is an alkyl group of 1 - 8 carbon atoms, particularly 1 - 4 carbon atoms, and R' is an alkyl group of 1 - 4 carbon atoms, specifically methyl or ethyl acetimidate.

  18. Interaction Between Toxic Metals and Complex Biofilm/Mineral/Solution

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Interfaces highlights title by Alexis S. Templeton, Thomas P. Trainor, and Gordon E. Brown, Jr., Stanford University Sorption reactions on particle surfaces can dramatically affect the speciation, cycling and bioavailability of essential micronutrients (i.e. PO43-, Cu, Zn etc.) and toxic metals and metalloids (i.e. Pb, Hg, Se, As) in soils and aquatic environments. Considerable attention has been focused on understanding metal sorption reactions at a molecular/mechanistic level and the

  19. Relationship Between Composition and Toxicity of Engine Emissions |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Emissions Relationship Between Composition and Toxicity of Engine Emissions 2004 Diesel Engine Emissions Reduction (DEER) Conference Presentation: Lovelace Respiratory Research Instiitute PDF icon 2004_deer_mcdonald.pdf More Documents & Publications The Effect of Changes in Diesel Exhaust Composition and After-Treatment Technology on Lung Inflammation and Resistance to Viral Infection Components Responsible for the Health Effects of Inhaled Engine Emissions

  20. Measurement of nitrogen content in a gas mixture by transforming the nitrogen into a substance detectable with nondispersive infrared detection

    DOE Patents [OSTI]

    Owen, Thomas E. (Helotes, TX); Miller, Michael A. (San Antonio, TX)

    2010-08-24

    A method of determining the amount of nitrogen in a gas mixture. The constituent gases of the mixture are dissociated and transformed to create a substance that may measured using nondispersive infrared adsorption techniques.

  1. Measurement of nitrogen content in a gas mixture by transforming the nitrogen into a substance detectable with nondispersive infrared detection

    DOE Patents [OSTI]

    Owen, Thomas E.; Miller, Michael A.

    2007-03-13

    A method of determining the amount of nitrogen in a gas mixture. The constituent gases of the mixture are dissociated and transformed to create a substance that may measured using nondispersive infrared adsorption techniques.

  2. Comparative developmental toxicity of environmentally relevant oxygenated PAHs

    SciTech Connect (OSTI)

    Knecht, Andrea L.; Goodale, Britton C.; Truong, Lisa; Simonich, Michael T.; Swanson, Annika J.; Matzke, Melissa M.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert L.

    2013-09-01

    Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are byproducts of combustion and photo-oxidation of parent PAHs. OPAHs are widely present in the environment and pose an unknown hazard to human health. The developing zebrafish was used to evaluate a structurally diverse set of 38 OPAHs for malformation induction, gene expression changes and mitochondrial function. Zebrafish embryos were exposed from 6 to 120 h post fertilization (hpf) to a dilution series of 38 different OPAHs and evaluated for 22 developmental endpoints. AHR activation was determined via CYP1A immunohistochemistry. Phenanthrenequinone (9,10-PHEQ), 1,9-benz-10-anthrone (BEZO), xanthone (XAN), benz(a)anthracene-7,12-dione (7,12-B[a]AQ), and 9,10-anthraquinone (9,10-ANTQ) were evaluated for transcriptional responses at 48 hpf, prior to the onset of malformations. qRT-PCR was conducted for a number of oxidative stress genes, including the glutathione transferase(gst), glutathione peroxidase(gpx), and superoxide dismutase(sod) families. Bioenergetics was assayed to measure in vivo oxidative stress and mitochondrial function in 26 hpf embryos exposed to OPAHs. Hierarchical clustering of the structure-activity outcomes indicated that the most toxic of the OPAHs contained adjacent diones on 6-carbon moieties or terminal, para-diones on multi-ring structures. 5-carbon moieties with adjacent diones were among the least toxic OPAHs while the toxicity of multi-ring structures with more centralized para-diones varied considerably. 9,10-PHEQ, BEZO, 7,12-B[a]AQ, and XAN exposures increased expression of several oxidative stress related genes and decreased oxygen consumption rate (OCR), a measurement of mitochondrial respiration. Comprehensive in vivo characterization of 38 structurally diverse OPAHs indicated differential AHR dependency and a prominent role for oxidative stress in the toxicity mechanisms. - Highlights: OPAHs are byproducts of combustion present in the environment. OPAHs pose a largely unknown hazard to human health. We assayed the developmental toxicology of 39 different OPAHs in zebrafish. The most toxic OPAHs contained adjacent diones or terminal, para-diones. AHR dependency varied among OPAHs, and oxidative stress influenced their toxicology.

  3. Evaluation of Chemical Warfare Agent Percutaneous Vapor Toxicity: Derivation of Toxicity Guidelines for Assessing Chemical Protective Ensembles.

    SciTech Connect (OSTI)

    Watson, A.P.

    2003-07-24

    Percutaneous vapor toxicity guidelines are provided for assessment and selection of chemical protective ensembles (CPEs) to be used by civilian and military first responders operating in a chemical warfare agent vapor environment. The agents evaluated include the G-series and VX nerve agents, the vesicant sulfur mustard (agent HD) and, to a lesser extent, the vesicant Lewisite (agent L). The focus of this evaluation is percutaneous vapor permeation of CPEs and the resulting skin absorption, as inhalation and ocular exposures are assumed to be largely eliminated through use of SCBA and full-face protective masks. Selection of appropriately protective CPE designs and materials incorporates a variety of test parameters to ensure operability, practicality, and adequacy. One aspect of adequacy assessment should be based on systems tests, which focus on effective protection of the most vulnerable body regions (e.g., the groin area), as identified in this analysis. The toxicity range of agent-specific cumulative exposures (Cts) derived in this analysis can be used as decision guidelines for CPE acceptance, in conjunction with weighting consideration towards more susceptible body regions. This toxicity range is bounded by the percutaneous vapor estimated minimal effect (EME{sub pv}) Ct (as the lower end) and the 1% population threshold effect (ECt{sub 01}) estimate. Assumptions of exposure duration used in CPE certification should consider that each agent-specific percutaneous vapor cumulative exposure Ct for a given endpoint is a constant for exposure durations between 30 min and 2 hours.

  4. Air toxics evaluation of ABB Combustion Engineering Low-Emission Boiler Systems

    SciTech Connect (OSTI)

    Wesnor, J.D.

    1993-10-26

    The specific goals of the program are to identify air toxic compounds that might be emmitted from the new boiler with its various Air Pollution Control device for APCD alternatives in levels of regulatory concern. For the compounds thought to be of concern, potential air toxic control methodologies will be suggested and a Test Protocol will be written to be used in the Proof of Concept and full scale tests. The following task was defined: Define Replations and Standards; Identify Air Toxic Pollutants of Interest to Interest to Utility Boilers; Assesment of Air Toxic By-Products; State of the Art Assessment of Toxic By-Product Control Technologies; and Test Protocol Definition.

  5. ROLE OF TOXICITY ASSESSMENT AND MONITORING IN MANAGING THE RECOVERY OF A WASTEWATER RECEIVING STREAM

    SciTech Connect (OSTI)

    Greeley Jr, Mark Stephen; Kszos, Lynn A; Stewart, Arthur J; Smith, John G

    2011-01-01

    We evaluate the roles of a long-term comprehensive toxicity assessment and monitoring program in management and for ecological recovery of a freshwater receiving stream impacted by industrial discharges and legacy contamination. National Pollution Discharge Elimination Permit (NPDES)-driven whole effluent toxicity (WET) tests using Ceriodaphnia and fathead minnows were conducted for more than twenty years to characterize wastewaters at the US National Nuclear Security Agency s Y-12 National Security Complex in Oak Ridge, Tennessee. Ambient toxicity tests also were conducted to assess water samples from EFPC, the stream receiving the wastewater discharges. The ambient tests were conducted as part of an extensive biological monitoring program that included routine surveys of fish, invertebrate and periphyton communities. WET testing, associated toxicant identification evaluations (TIEs), and ambient toxicity monitoring were instrumental in identifying toxicants and their sources at the Y-12 Complex, guiding modifications to wastewater treatment procedures, and assessing the success of various pollution-abatement actions. Through time, as requirements changed and water quality improved, the toxicity monitoring program became more focused. Ambient testing with Ceriodaphnia and fathead minnow larvae also was supplemented with less-standardized but more-sensitive alternative laboratory and in situ bioassays. The Y-12 Complex biological monitoring experience demonstrates the significant roles effluent and ambient toxicity testing can have in controlling and managing toxic discharges to receiving waters. It also emphasizes the value of supplementing WET and standardized ambient toxicity tests with alternative laboratory and in situ toxicity tests tailored to address specific problems.

  6. Object Modeling for Transport of Physical Objects or Substances Across a Geograc

    Energy Science and Technology Software Center (OSTI)

    1997-02-12

    TRANSPORTNET, a suite of object classes, addresses the general problem of simulating transport of objects or substances across a geographically distributed network. This highly abstract concept supports subclassed networks of many types, including road, rail, air, and navigation transportation networks, drainage (hydrological) networks, and utility networks of various sorts, such as pipelines, transmission lines, etc. In TRANSPORTNET, transport occurs along transport links which are connected by transport nodes. The concepts of link and node aremore » also highly abstracted and subject to several topological variants (subclasses), subject to the constraint that all transport takes place along links, and links are connected at the nodes.« less

  7. Mobile Source Air Toxics Rule (released in AEO2008)

    Reports and Publications (EIA)

    2008-01-01

    On February 9, 2007, the Environmental Protection Agency (EPA) released its MSAT2 rule, which will establish controls on gasoline, passenger vehicles, and portable fuel containers. The controls are designed to reduce emissions of benzene and other hazardous air pollutants. Benzene is a known carcinogen, and the EPA estimates that mobile sources produced more than 70% of all benzene emissions in 1999. Other mobile source air toxics, including 1,3-butadiene, formaldehyde, acetaldehyde, acrolein, and naphthalene, also are thought to increase cancer rates or contribute to other serious health problems.

  8. Pharmacokinetic drivers of toxicity for basic molecules: Strategy to lower pKa results in decreased tissue exposure and toxicity for a small molecule Met inhibitor

    SciTech Connect (OSTI)

    Diaz, Dolores; Ford, Kevin A.; Hartley, Dylan P.; Harstad, Eric B.; Cain, Gary R.; Achilles-Poon, Kirsten; Nguyen, Trung; Peng, Jing; Zheng, Zhong; Merchant, Mark; Sutherlin, Daniel P.; Gaudino, John J.; Kaus, Robert; Lewin-Koh, Sock C.; Choo, Edna F.; Liederer, Bianca M.; Dambach, Donna M.

    2013-01-01

    Several toxicities are clearly driven by free drug concentrations in plasma, such as toxicities related to on-target exaggerated pharmacology or off-target pharmacological activity associated with receptors, enzymes or ion channels. However, there are examples in which organ toxicities appear to correlate better with total drug concentrations in the target tissues, rather than with free drug concentrations in plasma. Here we present a case study in which a small molecule Met inhibitor, GEN-203, with significant liver and bone marrow toxicity in preclinical species was modified with the intention of increasing the safety margin. GEN-203 is a lipophilic weak base as demonstrated by its physicochemical and structural properties: high LogD (distribution coefficient) (4.3) and high measured pKa (7.45) due to the basic amine (N-ethyl-3-fluoro-4-aminopiperidine). The physicochemical properties of GEN-203 were hypothesized to drive the high distribution of this compound to tissues as evidenced by a moderately-high volume of distribution (Vd > 3 l/kg) in mouse and subsequent toxicities of the compound. Specifically, the basicity of GEN-203 was decreased through addition of a second fluorine in the 3-position of the aminopiperidine to yield GEN-890 (N-ethyl-3,3-difluoro-4-aminopiperidine), which decreased the volume of distribution of the compound in mouse (Vd = 1.0 l/kg), decreased its tissue drug concentrations and led to decreased toxicity in mice. This strategy suggests that when toxicity is driven by tissue drug concentrations, optimization of the physicochemical parameters that drive tissue distribution can result in decreased drug concentrations in tissues, resulting in lower toxicity and improved safety margins. -- Highlights: ? Lower pKa for a small molecule: reduced tissue drug levels and toxicity. ? New analysis tools to assess electrostatic effects and ionization are presented. ? Chemical and PK drivers of toxicity can be leveraged to improve safety.

  9. Respiratory symptoms among glass bottle makers exposed to stannic chloride solution and other potentially hazardous substances

    SciTech Connect (OSTI)

    Levy, B.S.; Davis, F.; Johnson, B.

    1985-04-01

    Concern about upper respiratory tract irritation and other symptoms among workers at a glass bottle manufacturing plant led to an epidemiologic and an industrial hygiene survey. Questionnaire responses from 35 hot end and 53 cold end workers indicated that the incidence of wheezing, chest pain, dyspnea on exertion, and cough was significantly elevated among hot end workers. Among both smokers and nonsmokers, hot end workers reported higher, but not significantly higher, rates of wheezing and chest pain. Among smokers, hot end workers reported significantly higher rates of dyspnea on exertion and cough than did cold end workers. Data suggest that reported exposure to stannic chloride solution likely caused these symptoms. The industrial hygiene survey, conducted when stannic chloride use had been reduced, cleaning had been done, and ventilation improved, focused on measuring air contaminants that might possibly cause symptoms. Levels of hydrogen chloride, which apparently was formed by the combination of stannic chloride and water in the presence of heat, were elevated. The finding of increased prevalence of respiratory symptoms among hot end workers was consistent with this exposure. Recommendations were made to reduce hazardous exposures at this plant. Individuals responsible for occupational health should be aware that relatively benign substances, such as stannic chloride and water, can combine spontaneously to form hazardous substances.

  10. Organic ligands reduce copper toxicity in Pseudomonas syringae

    SciTech Connect (OSTI)

    Azenha, M.; Vasconcelos, M.T.; Cabral, J.P.S.

    1995-03-01

    Pseudomonas syringae cells were exposed to 100 {mu}M copper alone, or to previously equilibrated copper sulfate-ligand solutions. Ligand concentrations were determined experimentally as those that reduced the free copper concentration to 5 {mu}M (determined with a Cu{sup 2+}-selective electrode). These values were in agreement with those calculated by computational equilibrium simulation based on published stability constants. Exposure of P. syringae cells to copper sulfate, chloride, or nitrate resulted in similar high mortality, suggesting that copper was responsible for cell death. Acetate, succinate, proline, lysine, cysteine, and EDTA significantly reduced both the amount of copper bound to the cells and cell death, indicating that not only strong chelating agents but also weak and moderate copper ligands can effectively antagonize copper toxicity. However, cysteine and EDTA were considerably more effective than acetate, succinate, proline, and lysine, indicating that copper toxicity is not simply a function of free copper concentration but depends on the nature of the ligand. The results suggested that a significant fraction of copper bound to acetate, succinate, proline, or lysine was displaced to the bacteria or, alternatively, mixed copper-ligand-cell complexes could be formed. On the contrary, none of these phenomena occurred for the copper complexes with cysteine or EDTA.

  11. Microbial stabilization and mass reduction of wastes containing radionuclides and toxic metals

    DOE Patents [OSTI]

    Francis, A.J.; Dodge, C.J.; Gillow, J.B.

    1991-09-10

    A process is provided to treat wastes containing radionuclides and toxic metals with Clostridium sp. BFGl to release a large fraction of the waste solids into solution and convert the radionuclides and toxic metals to a more concentrated and stable form with concurrent volume and mass reduction. The radionuclides and toxic metals being in a more stable form are available for recovery, recycling and disposal. 18 figures.

  12. Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Natural Gas (CNG)-Powered Vehicles | Department of Energy Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed Natural Gas (CNG)-Powered Vehicles Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed Natural Gas (CNG)-Powered Vehicles 2005 Diesel Engine Emissions Reduction (DEER) Conference Presentations and Posters PDF icon 2005_deer_mauderly.pdf More Documents & Publications Relationship Between Composition and Toxicity of Engine Emission Samples

  13. Early Proctoscopy is a Surrogate Endpoint of Late Rectal Toxicity in

    Office of Scientific and Technical Information (OSTI)

    Prostate Cancer Treated With Radiotherapy (Journal Article) | SciTech Connect Early Proctoscopy is a Surrogate Endpoint of Late Rectal Toxicity in Prostate Cancer Treated With Radiotherapy Citation Details In-Document Search Title: Early Proctoscopy is a Surrogate Endpoint of Late Rectal Toxicity in Prostate Cancer Treated With Radiotherapy Purpose: To predict the grade and incidence of late clinical rectal toxicity through short-term (1 year) mucosal alterations. Methods and Materials:

  14. Microbial stabilization and mass reduction of wastes containing radionuclides and toxic metals

    DOE Patents [OSTI]

    Francis, Arokiasamy J. (Middle Island, NY); Dodge, Cleveland J. (Wading River, NY); Gillow, Jeffrey B. (Valley Cottage, NY)

    1991-01-01

    A process is provided to treat wastes containing radionuclides and toxic metals with Clostridium sp. BFGl to release a large fraction of the waste solids into solutin and convert the radionuclides and toxic metals to a more concentrated and stable form with concurrent volume and mass reduction. The radionuclides and toxic metals being in a more stable form are available for recovery, recycling and disposal.

  15. Summary of EPA Final Rules for Air Toxic Standards for Industrial,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Commercial, and Institutional (ICI) Boilers and Process Heaters, February 2013 | Department of Energy Summary of EPA Final Rules for Air Toxic Standards for Industrial, Commercial, and Institutional (ICI) Boilers and Process Heaters, February 2013 Summary of EPA Final Rules for Air Toxic Standards for Industrial, Commercial, and Institutional (ICI) Boilers and Process Heaters, February 2013 This summary of EPA Final Rules for Air Toxic Standards for Industrial, Commercial, and Institutional

  16. Pentose fermentation of normally toxic lignocellulose prehydrolysate with strain of Pichia stipitis yeast using air

    DOE Patents [OSTI]

    Keller, Jr., Fred A. (Lakewood, CO); Nguyen, Quang A. (Golden, CO)

    2002-01-01

    Strains of the yeast Pichia stipitis NPw9 (ATCC PTA-3717) useful for the production of ethanol using oxygen for growth while fermenting normally toxic lignocellulosic prehydrolysates.

  17. Summary of EPA Final Rules for Air Toxic Standards for Industrial...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Summary of EPA Final Rules for Air Toxic Standards for Industrial, Commercial, and Institutional (ICI) Boilers and Process Heaters, February 2013 Summary of EPA Final Rules for Air ...

  18. On-Line Microbial Whole Effluent Toxicity Monitoring for Industrial Wastewater

    SciTech Connect (OSTI)

    Mathews, S; Hoppes, W; Mascetti, M; Campbell, C G

    2002-09-17

    In this study a respirometer is tested for its ability to act as an early upset warning device and whole effluent toxicity monitor for industrial discharge. Industrial discharge water quality is commonly evaluated by comparing measured chemical concentrations to target values or regulatory limits established by governmental agencies. Unless the regulatory values are based upon empirical data, the actual effect of the discharge on aquatic systems is unknown. At the same time assessing the environmental toxicology of wastewater discharges is complicated by synergistic relationships among chemical constituents producing greater total toxicity. For example, metals may be more toxic in waters with low total hardness or more soluble at lower pH. An alternative approach that we are investigating is whole effluent toxicity testing. This study investigates the measurement of whole effluent toxicity through an on-line respirometer that measures toxicity to microorganisms comprising activated sludge. In this approach the oxygen uptake rate is monitored and used as an indicator of microbial activity or health. This study investigates the use of an online whole effluent toxicity testing system to provide early upset warning and the consistency of measured response to low pH. Repeated exposure of the microorganisms to low pH results in reduced sensitivity of the microbial population. We investigate whether this reduction in sensitivity results from physiological acclimation or changes in species composition. We identify promising applications, where, with proper calibration, respirometry based toxicity monitoring appear to be well suited for relative comparisons of whole effluent toxicity.

  19. Device of dispensing micro doses of aqueous solutions of substances onto a carrier and device for carrying out said method

    DOE Patents [OSTI]

    Ershow, Gennady Moiseevich (Moscow, RU); Kirillov, Evgenii Vladislavovich (gor. Dolgoprudnyi, RU); Mirzabekov, Andrei Darievich (Moscow, RU)

    1998-01-01

    A device for dispensing microdoses of aqueous solutions are provided, whereby the substance is transferred by the free surface end of a rodlike transferring element; the temperature of the transferring element is maintained at essentially the dew point of the ambient air during the transfer. The device may comprise a plate-like base to which are affixed a plurality of rods; the unfixed butt ends of the rods are coplanar. The device further comprises a means for maintaining the temperature of the unfixed butt ends of the rods essentially equal to the dew point of the ambient air during transfer of the aqueous substance.

  20. Method and apparatus for diagnosis of lead toxicity

    DOE Patents [OSTI]

    Rosen, John F. (Riverside, CT); Slatkin, Daniel N. (Bayside, NY); Wielopolski, Lucian (Shirley, NY)

    1989-01-01

    Improved methods and apparatus for in vivo measurement of the skeletal lead burden of a patient and for diagnosis of lead toxicity are disclosed. The apparatus comprises an x-ray tube emitting soft low energy x-rays from a silver anode, a polarizer for polarizing the emitted x-rays, and a detector for detecting photons fluoresced from atoms in the patient's tibia upon irradiation by the polarized x-rays. The fluoresced photons are spectrally analyzed to determine their energy distribution. Peaks indicating the presence of lead are identified if the patient has relatively high bone lead content. The data may be compared to data recorded with respect to a similar test performed on patients having also had the conventional EDTA chelation tests performed thereon in order to correlate the test results with respect to a particular patient to the conventionally accepted EDTA chelation test.

  1. Microcurrent therapeutic technique for treatment of radiation toxicity

    DOE Patents [OSTI]

    Lennox, Arlene (Elburn, IL); Funder, Sandra (Crown Point, IL)

    2000-01-01

    The present technique provides a method of remediating the toxicities associated with radiation therapy. A conductive gel is applied to the affected bodily area. A sinusoidally pulsed biphasic DC current is then applied to the affected bodily area using at least one electrode. The electrode is manipulated using active tactile manipulation by for a predetermined time and the frequency of the sinusoidally pulsed biphasic DC current is decreased during the course of the treatment. The method also includes applying a spiked pulsed biphasic DC current to the affected bodily area using at least one electrode. This electrode is also manipulated using active tactile manipulation by for a predetermined time and the frequency of the spiked pulsed biphasic DC current is also decreased during the course of the treatment.

  2. Physicochemical properties and toxicities of hydrophobicpiperidinium and pyrrolidinium ionic liquids

    SciTech Connect (OSTI)

    Salminen, Justin; Papaiconomou, Nicolas; Kumar, R. Anand; Lee,Jong-Min; Kerr, John; Newman, John; Prausnitz, John M.

    2007-06-25

    Some properties are reported for hydrophobic ionic liquids (IL) containing 1-methyl-1-propyl pyrrolidinium [MPPyrro]{sup +}, 1-methyl-1-butyl pyrrolidinium [MBPyrro]{sup +}, 1-methyl-1-propyl piperidinium [MPPip]{sup +}, 1-methyl-1-butyl piperidinium [MBPip]{sup +}, 1-methyl-1-octylpyrrolidinium [MOPyrro]{sup +} and 1-methyl-1-octylpiperidinium [MOPip]{sup +} cations. These liquids provide new alternatives to pyridinium and imidazolium ILs. High thermal stability of an ionic liquid increases safety in applications like rechargeable lithium-ion batteries and other electrochemical devices. Thermal properties, ionic conductivities, viscosities, and mutual solubilities with water are reported. In addition, toxicities of selected ionic liquids have been measured using a human cancer cell-line. The ILs studied here are sparingly soluble in water but hygroscopic. We show some structure-property relationships that may help to design green solvents for specific applications. While ionic liquids are claimed to be environmentally-benign solvents, as yet few data have been published to support these claims.

  3. The Treatment of Mixed Waste with GeoMelt In-Container Vitrification

    SciTech Connect (OSTI)

    Finucane, K.G.; Campbell, B.E.

    2006-07-01

    AMEC's GeoMelt{sup R} In-Container Vitrification (ICV){sup TM} has been used to treat diverse types of mixed low-level radioactive waste. ICV is effective in the treatment of mixed wastes containing polychlorinated biphenyls (PCBs) and other semi-volatile organic compounds, volatile organic compounds (VOCs) and heavy metals. The GeoMelt vitrification process destroys organic compounds and immobilizes metals and radionuclides in an extremely durable glass waste form. The process is flexible allowing for treatment of aqueous, oily, and solid mixed waste, including contaminated soil. In 2004, ICV was used to treat mixed radioactive waste sludge containing PCBs generated from a commercial cleanup project regulated by the Toxic Substances Control Act (TSCA), and to treat contaminated soil from Rocky Flats Environmental Technology Site. The Rocky Flats soil contained cadmium, PCBs, and depleted uranium. In 2005, AMEC completed a treatability demonstration of the ICV technology on Mock High Explosive from Sandia National Laboratories. This paper summarizes results from these mixed waste treatment projects. (authors)

  4. Basic Engineering Research for D and D of R Reactor Storage Pond Sludge: Electrokinetics, Carbon Dioxide Extraction, and Supercritical Water Oxidation

    SciTech Connect (OSTI)

    Michael A. Matthews; David A. Bruce,; Thomas A. Davis; Mark C. Thies; John W. Weidner; Ralph E. White

    2002-04-01

    Large quantities of mixed low level waste (MLLW) that fall under the Toxic Substances Control Act (TSCA) exist and will continue to be generated during D and D operations at DOE sites across the country. The standard process for destruction of MLLW is incineration, which has an uncertain future. The extraction and destruction of PCBs from MLLW was the subject of this research Supercritical Fluid Extraction (SFE) with carbon dioxide with 5% ethanol as cosolvent and Supercritical Waster Oxidation (SCWO) were the processes studied in depth. The solid matrix for experimental extraction studies was Toxi-dry, a commonly used absorbent made from plant material. PCB surrogates were 1.2,4-trichlorobenzene (TCB) and 2-chlorobiphenyl (2CBP). Extraction pressures of 2,000 and 4,000 psi and temperatures of 40 and 80 C were studied. Higher extraction efficiencies were observed with cosolvent and at high temperature, but pressure little effect. SCWO treatment of the treatment of the PCB surrogates resulted in their destruction below detection limits.

  5. Federal Environmental Permitting Handbook. Environmental Guidance

    SciTech Connect (OSTI)

    Not Available

    1991-05-01

    The handbook consists of eight chapters addressing permitting and licensing requirements under the Resource Conservation and Recovery Act (RCRA), the Comprehensive Environmental Response, Compensation, and Liability Act of 1980, as amended by the Superfund Amendments and Reauthorization Act of 1986 (CERCLA/SARA), the Atomic Energy Act (AEA), the Clean Air Act (CAA), the Clean Water Act (CWA), the Federal Insectide, Fungicide, and Rodenticide Act (FIFRA), the Safe Drinking Water Act (SDWA), and the Toxic Substances Control Act (TSCA). Each chapter consists of: (1) an introduction to the statute and permitting requirements; (2) a diagram illustrating the relationship between permitting requirements under the statute being discussed and permitting requirements from other environmental statutes which may have to be addressed when applying for a particular permit (e.g., when applying for a RCRA permit, permits and permit applications under the CWA, CAA, SDWA, etc. would have to be listed in the RCRA permit application); and, (3) a compilation of the permitting requirements for the regulatory program resulting from the statute. In addition, the Handbook contains a permitting keyword index and a listing of hotline telephone numbers for each of the statutes.

  6. Superfund record of decision (EPA region 10): Eielson Air Force Base, Fairbanks-North Star Borough, AK, September 30, 1996

    SciTech Connect (OSTI)

    1997-10-01

    The decision document presents the final remedial action selected for Eielson Air Force Base (AFB), Alaska. The sitewide investigation at Eielson AFB evaluated basewide contamination that is not confined or attributable to specific source areas identified and addressed in the FFA as well as cumulative risks to human health and the environment posed by contamination on a sitewide basis. Garrison Slough is the only one that poses an unacceptable risk to human health and the environment. Polychlorinated biphenyls (PCBs) were found in the fish tissue and sediments of Garrison Slough. Soils in a trench adjacent to Garrison Slough were contaminated with PCBs and appear to be the source of contamination to slough sediments via surface water runoff. The major components of the selected remedy include: Fishing restrictions in Garrison Slough; Fish control device near the downstream edge of Eielson AFB; Excavation of contaminated soils and sediments with concentrations greater than 10 mg/kg PCBs; Onsite disposal of material with PCB concentrations less than 50 mg/kg; Offsite disposal or treatment of materials with PCB concentrations greater than 50 mg/kg in accordance with the Toxic Substances Control Act (TSCA), 40 CFR part 761; and Environmental monitoring of soils, sediments, surface water, fish, and groundwater.

  7. Waste Isolation Pilot Plant Biennial Environmental Compliance Report

    SciTech Connect (OSTI)

    Washington Regulatory and Environmental Services

    2006-10-12

    This Biennial Environmental Compliance Report (BECR) documents compliance with environmental regulations at the Waste Isolation Pilot Plant (WIPP), a facility designed and authorized for the safe disposal of transuranic (TRU) radioactive waste. This BECR covers the reporting period from April 1, 2004, to March 31, 2006. As required by the WIPP Land Withdrawal Act (LWA) (Public Law [Pub. L.] 102-579, as amended by Pub. L. 104-201), the BECR documents United States (U.S.) Department of Energy (DOE) compliance with regulations and permits issued pursuant to the following: (1) Title 40 Code of Federal Regulations (CFR) Part 191, Subpart A, "Environmental Standards for Management and Storage"; (2) Clean Air Act (CAA) (42 United States Code [U.S.C.] 7401, et seq.); (3) Solid Waste Disposal Act (SWDA) (42 U.S.C. 6901-6992, et seq.); (4) Safe Drinking Water Act (SDWA) (42 U.S.C. 300f, et seq.); (5) Toxic Substances Control Act (TSCA) (15 U.S.C. 2601, et seq.); (6) Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) (42 U.S.C. 9601, et seq.); and all other federal and state of New Mexico laws pertaining to public health and safety or the environment.

  8. Apparatus and methods for monitoring the concentrations of hazardous airborne substances, especially lead

    DOE Patents [OSTI]

    Zaromb, Solomon

    2004-07-13

    Air is sampled at a rate in excess of 100 L/min, preferably at 200-300 L/min, so as to collect therefrom a substantial fraction, i.e., at least 20%, preferably 60-100%, of airborne particulates. A substance of interest (analyte), such as lead, is rapidly solubilized from the the collected particulates into a sample of liquid extractant, and the concentration of the analyte in the extractant sample is determined. The high-rate air sampling and particulate collection may be effected with a high-throughput filter cartridge or with a recently developed portable high-throughput liquid-absorption air sampler. Rapid solubilization of lead is achieved by a liquid extractant comprising 0.1-1 M of acetic acid or acetate, preferably at a pH of 5 or less and preferably with inclusion of 1-10% of hydrogen peroxide. Rapid determination of the lead content in the liquid extractant may be effected with a colorimetric or an electroanalytical analyzer.

  9. Results of Toxicity Studies Conducted on Outfall X-08 and Its Contributing Waste Streams, November 1999 - June 2000

    SciTech Connect (OSTI)

    Specht, W.L.

    2000-06-28

    This interim report summarizes the results of toxicity tests, Toxicity Identification Evaluations, and chemical analyses that have been conducted on SRS's NPDES Outfall X-08 and its contributing waste streams between November 1999 and June 2000.

  10. Effects of water hardness on the toxicity of manganese to developing brown trout (Salmo trutta)

    SciTech Connect (OSTI)

    Stubblefield, W.A.; Garrison, T.D.; Hockett, J.R.; Brinkman, S.F.; Davies, P.H.; McIntyre, M.W.

    1997-10-01

    Manganese is a common constituent of point and nonpoint discharges from mining and smelting activities. Available data indicate that Mn is acutely toxic at relatively high aqueous concentrations, when compared with trace metals, and its toxicity is affected by water hardness. Little information is available regarding the chronic toxicity of manganese. Early-life-stage (ELS) tests were conducted to determine the toxicity of manganese to brown trout (Salmo trutta) and to evaluate the extent to which water hardness (ranging from 30 to 450 mg/L as CaCO{sub 3}) affects the chronic toxicity of Mn. Water hardness of significantly affected Mn chronic toxicity, with toxicity decreasing with increasing hardness. Decreased survival was the predominant effect noted in the 30-mg/L hardness experiment, while significant effects on growth (as measured by changes in body weight) were observed in both the 150- and 450-mg/L hardness experiments. Twenty-five percent inhibition concentration (IC25) values, based on the combined endpoints (i.e., survival and body weight), were 4.67, 5.59, and 8.68 mg Mn/L (based on measured Mn concentration) at hardness levels of approximately 30, 150, and 450 mg/L as CaCO{sub 3}, respectively.

  11. Acute toxicity of selected metals and phenols on RTG-2 and CHSE-214 fish cell lines

    SciTech Connect (OSTI)

    Castano, A. |; Vega, M.M.; Tarazona, J.V.

    1995-08-01

    In vitro toxicity tests with fish cell lines appear as an alternative to single species bioassays and have been used successfully in different applications, both for single chemicals and for environmental samples, including Toxicity Identification Evaluation procedures. Different fish cell lines, such as BF-2, RTG-2, FHM and R1 have been employed in these toxicological studies. The aim of the present study was to compare the sensitivity of two salmonid fish cell lines, RTG-2 and CHSE-214, in evaluating toxicity of seven compounds (three metals and four phenolic chemicals), by measuring three endpoints: cellular mass, cell viability and intracellular ATP content of the cells. 22 refs., 3 figs., 1 tab.

  12. Proteomic analysis of rat cerebral cortex following subchronic acrolein toxicity

    SciTech Connect (OSTI)

    Rashedinia, Marzieh; Lari, Parisa; Abnous, Khalil; Hosseinzadeh, Hossein

    2013-10-01

    Acrolein, a member of reactive ?,?-unsaturated aldehydes, is a major environmental pollutant. Acrolein is also produced endogenously as a toxic by-product of lipid peroxidation. Because of high reactivity, acrolein may mediate oxidative damages to cells and tissues. It has been shown to be involved in a wide variety of pathological states including pulmonary, atherosclerosis and neurodegenerative diseases. In this study we employed proteomics approach to investigate the effects of subchronic oral exposures to 3 mg/kg of acrolein on protein expression profile in the brain of rats. Moreover effects of acrolein on malondialdehyde (MDA) levels and reduced glutathione (GSH) content were investigated. Our results revealed that treatment with acrolein changed levels of several proteins in diverse physiological process including energy metabolism, cell communication and transport, response to stimulus and metabolic process. Interestingly, several differentially over-expressed proteins, including ?-synuclein, enolase and calcineurin, are known to be associated with human neurodegenerative diseases. Changes in the levels of some proteins were confirmed by Western blot. Moreover, acrolein increases the level of MDA, as a lipid peroxidation biomarker and decreased GSH concentrations, as a non-enzyme antioxidant in the brain of acrolein treated rats. These findings suggested that acrolein induces the oxidative stress and lipid peroxidation in the brain, and so that may contribute to the pathophysiology of neurological disorders. - Highlights: Acrolein intoxication increased lipid peroxidation and deplete GSH in rat brain. Effect of acrolein on protein levels of cerebral cortex was analyzed by 2DE-PAGE. Levels of a number of proteins with different biological functions were increased.

  13. Analysis of the toxicity in Rocky Flats Plant surface water through a correlation between the whole effluent toxicity test and the Microtox assay

    SciTech Connect (OSTI)

    Ford, S.M.; Wolaver, H.A.; Figueroa, L.A.

    1992-07-01

    Results were correlated from the Microtox assay and the whole effluent acute toxicity test for effluents from the (1) wastewater treatment plant (WWTP) and (2) terminal ponds located at the Rocky Flats Plant. Literature reviews indicate that Photobacterium phosphoreum (Microtox assay) may be used as screening test for the reaction of Ceriodaphnia dubia and Pimephales promelas to toxins present in effluents. This study indicates that the Microtox is less sensitive to toxins present in the WWTP effluent than other test organisms (Ceriodaphnia dubia and Pimephales promelas). Toxicity appears to be from unionized ammonia. Ten months of data reveal that the surface water effluents which leave Rocky Flats boundaries are non-toxic when judged by all three test organisms.

  14. Analysis of the toxicity in Rocky Flats Plant surface water through a correlation between the whole effluent toxicity test and the Microtox assay

    SciTech Connect (OSTI)

    Ford, S.M.; Wolaver, H.A. ); Figueroa, L.A. )

    1992-01-01

    Results were correlated from the Microtox assay and the whole effluent acute toxicity test for effluents from the (1) wastewater treatment plant (WWTP) and (2) terminal ponds located at the Rocky Flats Plant. Literature reviews indicate that Photobacterium phosphoreum (Microtox assay) may be used as screening test for the reaction of Ceriodaphnia dubia and Pimephales promelas to toxins present in effluents. This study indicates that the Microtox is less sensitive to toxins present in the WWTP effluent than other test organisms (Ceriodaphnia dubia and Pimephales promelas). Toxicity appears to be from unionized ammonia. Ten months of data reveal that the surface water effluents which leave Rocky Flats boundaries are non-toxic when judged by all three test organisms.

  15. Tubular structured hierarchical mesoporous titania material derived from natural cellulosic substances and application as photocatalyst for degradation of methylene blue

    SciTech Connect (OSTI)

    Huang, Haiqing; Liu, Xiaoyan; Huang, Jianguo

    2011-11-15

    Graphical abstract: Bio-inspired, tubular structured hierarchical mesoporous titania material with high photocatalytic activity under UV light was fabricated employing natural cellulosic substance (cotton) as hard template and cetyltrimethylammonium bromide (CTAB) surfactant as soft template using a one-pot sol-gel method. Highlights: {yields} Tubular structured mesoporous titania material was fabricated by sol-gel method. {yields} The titania material faithfully recorded the hierarchical structure of the template substrate (cotton). {yields} The titania material exhibited high photocatalytic activity in decomposition of methylene blue. -- Abstract: Bio-inspired, tubular structured hierarchical mesoporous titania material was designed and fabricated employing natural cellulosic substance (cotton) as hard template and cetyltrimethylammonium bromide (CTAB) surfactant as soft template by one-pot sol-gel method. The tubular structured hierarchical mesoporous titania material processes large specific surface area (40.23 m{sup 2}/g) and shows high photocatalytic activity in the photodegradation of methylene blue under UV light irradiation.

  16. On using rational enzyme redesign to improve enzyme-mediated microbial dehalogenation of recalcitrant substances in deep-subsurface environments

    SciTech Connect (OSTI)

    Ornstein, R.L.

    1993-06-01

    Heavily halogenated hydrocarbons are one of the most prevalent classes of man-made recalcitrant environmental contaminants and often make their way into subsurface environments. Biodegradation of heavily chlorinated compounds in the deep subsurface often occurs at extremely slow rates because native enzymes of indigenous microbes are unable to efficiently metabolize such synthetic substances. Cost-effective engineering solutions do not exist for dealing with disperse and recalcitrant pollutants in the deep subsurface (i.e., ground water, soils, and sediments). Timely biodegradation of heavily chlorinated compounds in the deep subsurface may be best accomplished by rational redesign of appropriate enzymes that enhance the ability of indigenous microbes to metabolize these substances. The isozyme family cytochromes P450 are catalytically very robust and are found in all aerobic life forms and may be active in may anaerobes as well. The author is attempting to demonstrate proof-of-principle rational enzyme redesign of cytochromes P450 to enhance biodehalogenation.

  17. WAC 173-460 - Controls for New Sources of Toxic Air Pollutants...

    Open Energy Info (EERE)

    73-460 - Controls for New Sources of Toxic Air Pollutants Jump to: navigation, search OpenEI Reference LibraryAdd to library Legal Document- RegulationRegulation: WAC 173-460 -...

  18. Microsoft PowerPoint - Marcinowski April 2010 (Rev3).ppt [Compatibilit...

    Office of Environmental Management (EM)

    to ensure all disposal alternatives are evaluated Recent developments and changes... * Toxic Substances Control Act Incinerator at Oak * Toxic Substances Control Act Incinerator...

  19. A Review of Toxicity and Use and Handling Considerations for Guanidine, Guanidine Hydrochloride, and Urea.

    SciTech Connect (OSTI)

    Ertell, Katherine GB

    2006-03-27

    This is a technical report prepared for Oregon Sustainable Energy, LLC, under Agreement 06-19 with PNNL's Office of Small Business Programs. The request was to perform a review of the toxicity and safe handling of guanidine. The request was later amended to add urea. This report summarizes the toxicity data available in the scientific literature and provides an interpretation of the results and recommendations for handling these compounds.

  20. Time-series product and substance flow analyses of end-of-life electrical and electronic equipment in China

    SciTech Connect (OSTI)

    Habuer, Nakatani, Jun; Moriguchi, Yuichi

    2014-02-15

    Highlights: We estimate the possession and obsolescence of household appliances (HAs) in China. Over 4.85.1 billion units of major HAs will be discarded in the next 20 years. We calculate the amounts of substances contained in end-of-life (EoL) TV sets. Less common metals will tend to decrease in content in generation of EoL TV sets. Precious metals will tend to increase in content in EoL TV sets in 20152030. - Abstract: Given the amounts of end-of-life electrical and electronic equipment (EoL-EEE) being generated and their contents of both harmful and valuable materials, the EoL-EEE issue should be regarded not only as an emerging environmental problem but also as a resource management strategy in China. At present, in order to provide the basis for managing EoL-EEE at both product and substance levels in China, it is necessary to carry out a quantitative analysis on EoL-EEE and to determine how much of it will be generated and how much materials and substances it contains. In this study, the possession and obsolescence amounts of five types of household appliance (HA) including television (TV) sets and the amounts of substances contained in EoL TV sets were estimated using time-series product flow analysis (PFA) and substance flow analysis (SFA). The results of PFA indicated that the total possession amounts of those five types of HAs will exceed 3.1 billion units in 2030, which will be two times higher than those in 2010. In addition, it was estimated that cumulatively over 4.85.1 billion units of these five types of EoL HA would be obsoleted between 20102030. The results of SFA on TV sets indicated that the generated amounts of most of the less common metals and a part of common metals such as copper (Cu) would tend to decrease, whereas those of other common metals such as iron (Fe) as well as precious metals would tend to increase in EoL TV sets in 20152030. The results of this study provide a quantitative basis for helping decision makers develop strategic policies for the management of EoL-EEE considering both environmental and resource aspects. Moreover, a calculation scheme of obsolete HAs presented in this study can be applied to estimate other types of EoL durable good. Meanwhile, the frameworks of this study will help not only the policy decision makers in the Chinese government but also those in developing countries that are facing similar problems.

  1. Modifications to toxic CUG RNAs induce structural stability, rescue mis-splicing in a myotonic dystrophy cell model and reduce toxicity in a myotonic dystrophy zebrafish model

    SciTech Connect (OSTI)

    deLorimier, Elaine; Coonrod, Leslie A.; Copperman, Jeremy; Taber, Alex; Reister, Emily E.; Sharma, Kush; Todd, Peter K.; Guenza, Marina G.; Berglund, J. Andrew

    2014-10-10

    In this study, CUG repeat expansions in the 3' UTR of dystrophia myotonica protein kinase (DMPK) cause myotonic dystrophy type 1 (DM1). As RNA, these repeats elicit toxicity by sequestering splicing proteins, such as MBNL1, into proteinRNA aggregates. Structural studies demonstrate that CUG repeats can form A-form helices, suggesting that repeat secondary structure could be important in pathogenicity. To evaluate this hypothesis, we utilized structure-stabilizing RNA modifications pseudouridine (?) and 2'-O-methylation to determine if stabilization of CUG helical conformations affected toxicity. CUG repeats modified with ? or 2'-O-methyl groups exhibited enhanced structural stability and reduced affinity for MBNL1. Molecular dynamics and X-ray crystallography suggest a potential water-bridging mechanism for ?-mediated CUG repeat stabilization. ? modification of CUG repeats rescued mis-splicing in a DM1 cell model and prevented CUG repeat toxicity in zebrafish embryos. This study indicates that the structure of toxic RNAs has a significant role in controlling the onset of neuromuscular diseases.

  2. Modifications to toxic CUG RNAs induce structural stability, rescue mis-splicing in a myotonic dystrophy cell model and reduce toxicity in a myotonic dystrophy zebrafish model

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    deLorimier, Elaine; Coonrod, Leslie A.; Copperman, Jeremy; Taber, Alex; Reister, Emily E.; Sharma, Kush; Todd, Peter K.; Guenza, Marina G.; Berglund, J. Andrew

    2014-10-10

    In this study, CUG repeat expansions in the 3' UTR of dystrophia myotonica protein kinase (DMPK) cause myotonic dystrophy type 1 (DM1). As RNA, these repeats elicit toxicity by sequestering splicing proteins, such as MBNL1, into protein–RNA aggregates. Structural studies demonstrate that CUG repeats can form A-form helices, suggesting that repeat secondary structure could be important in pathogenicity. To evaluate this hypothesis, we utilized structure-stabilizing RNA modifications pseudouridine (Ψ) and 2'-O-methylation to determine if stabilization of CUG helical conformations affected toxicity. CUG repeats modified with Ψ or 2'-O-methyl groups exhibited enhanced structural stability and reduced affinity for MBNL1. Molecular dynamicsmore » and X-ray crystallography suggest a potential water-bridging mechanism for Ψ-mediated CUG repeat stabilization. Ψ modification of CUG repeats rescued mis-splicing in a DM1 cell model and prevented CUG repeat toxicity in zebrafish embryos. This study indicates that the structure of toxic RNAs has a significant role in controlling the onset of neuromuscular diseases.« less

  3. The underlying toxicological mechanism of chemical mixtures: A case study on mixture toxicity of cyanogenic toxicants and aldehydes to Photobacterium phosphoreum

    SciTech Connect (OSTI)

    Tian, Dayong; Lin, Zhifen; Zhou, Xianghong; Yin, Daqiang

    2013-10-15

    Intracellular chemical reaction of chemical mixtures is one of the main reasons that cause synergistic or antagonistic effects. However, it still remains unclear what the influencing factors on the intracellular chemical reaction are, and how they influence on the toxicological mechanism of chemical mixtures. To reveal this underlying toxicological mechanism of chemical mixtures, a case study on mixture toxicity of cyanogenic toxicants and aldehydes to Photobacterium phosphoreum was employed, and both their joint effects and mixture toxicity were observed. Then series of two-step linear regressions were performed to describe the relationships between joint effects, the expected additive toxicities and descriptors of individual chemicals (including concentrations, binding affinity to receptors, octanol/water partition coefficients). Based on the quantitative relationships, the underlying joint toxicological mechanisms were revealed. The result shows that, for mixtures with their joint effects resulting from intracellular chemical reaction, their underlying toxicological mechanism depends on not only their interaction with target proteins, but also their transmembrane actions and their concentrations. In addition, two generic points of toxicological mechanism were proposed including the influencing factors on intracellular chemical reaction and the difference of the toxicological mechanism between single reactive chemicals and their mixtures. This study provided an insight into the understanding of the underlying toxicological mechanism for chemical mixtures with intracellular chemical reaction. - Highlights: Joint effects of nitriles and aldehydes at non-equitoxic ratios were determined. A novel descriptor, ligandreceptor interaction energy (E{sub binding}), was employed. Quantitative relationships for mixtures were developed based on a novel descriptor. The underlying toxic mechanism was revealed based on quantitative relationships. Two generic points of toxicological mechanism were elucidated.

  4. 2008 Toxic Chemical Release Inventory 2008 Toxic Chemical Release Inventory Community Right-to-Know Act of 1986, Title III, Section 313

    SciTech Connect (OSTI)

    Ecology and Air Quality Group

    2009-10-01

    For reporting year 2008, Los Alamos National Laboratory (LANL) submitted a Form R report for lead as required under the Emergency Planning and Community Right-to- Know Act (EPCRA) Section 313. No other EPCRA Section 313 chemicals were used in 2008 above the reportable thresholds. This document was prepared to provide a description of the evaluation of EPCRA Section 313 chemical use and threshold determinations for LANL for calendar year 2008, as well as to provide background information about data included on the Form R reports. Section 313 of EPCRA specifically requires facilities to submit a Toxic Chemical Release Inventory Report (Form R) to the U.S. Environmental Protection Agency (EPA) and state agencies if the owners and operators manufacture, process, or otherwise use any of the listed toxic chemicals above listed threshold quantities. EPA compiles this data in the Toxic Release Inventory database. Form R reports for each chemical over threshold quantities must be submitted on or before July 1 each year and must cover activities that occurred at the facility during the previous year. In 1999, EPA promulgated a final rule on persistent bioaccumulative toxics (PBTs). This rule added several chemicals to the EPCRA Section 313 list of toxic chemicals and established lower reporting thresholds for these and other PBT chemicals that were already reportable. These lower thresholds became applicable in reporting year 2000. In 2001, EPA expanded the PBT rule to include a lower reporting threshold for lead and lead compounds. Facilities that manufacture, process, or otherwise use more than 100 lb of lead or lead compounds must submit a Form R.

  5. Toxicity of contaminants in lagoons and pannes of the Indiana Dunes National Lakeshore

    SciTech Connect (OSTI)

    Gillespie, R.; Speelman, J. [Indiana Univ.-Purdue Univ., Fort Wayne, IN (United States); Stewart, P.M. [National Biological Service, Porter, IN (United States). Indiana Dunes National Lakeshore

    1995-12-31

    Contaminants in water and sediments of lagoons and pannes were 2--90 times greater at sites adjacent to slag and coal piles than those at reference sites. One site (Lagoon-US5) had sediments with very high concentrations of toxic organics (e.g. naphthalene, phenanthrene, dibenzofuran). Although analyses indicated a gradient of contaminant concentration with distance from their sources, toxicity assays were somewhat equivocal. With the exception of less reproduction in Ceriodaphnia at one lagoon site (US3 = 0.55 of reference), survival of fathead minnows and reproduction in Ceriodaphnia in lagoon and panne waters varied independently of the contaminant concentration. In fact, there was better Ceriodaphnia reproduction in water from two contaminated sites (Lagoon-US5, Panne-WP1) than in water from reference sites. Fathead minnow survival, Ceriodaphnia survival, Ceriodaphnia reproduction, amphipod survival, and amphipod growth varied among sites in toxicity assays with sediments, 100% mortality of fatheads at Lagoon-US5, 100% mortality of Ceriodaphnia at Lagoon-US3, and less survival of fathead minnows at Lagoon-US3 indicate possible toxicity from contaminants in sediments at these sites. Of all organisms and end-points tested, Ceriodaphnia survival seemed to be most closely associated with concentrations of contaminants in lagoon water and sediments. Amphipod survival also varied with contaminants in sediments, however, survival in sediments of contaminated sites ranged only from 0.90--0.93 of reference sites. Although the results are not consistent among organisms, toxicity assays indicate that sediments from the lagoon site with the highest contaminants (Lagoon-US5) and possibly those from another contaminated lagoon site (Lagoon-US3) could be toxic to aquatic organisms. Water and sediments from contaminated panne sites do not appear to be toxic to aquatic test organisms.

  6. Method of in situ retrieval of contaminants or other substances using a barrier system and leaching solutions

    DOE Patents [OSTI]

    Nickelson, Reva A.; Walsh, Stephanie; Richardson, John G.; Dick, John R.; Sloan, Paul A.

    2006-12-26

    Processes and methods relating to treating contaminants and collecting desired substances from a zone of interest using subterranean collection and containment barriers. Tubular casings having interlock structures are used to create subterranean barriers for containing and treating buried waste and its effluents. The subterranean barrier includes an effluent collection system. Treatment solutions provided to the zone of interest pass therethrough and are collected by the barrier and treated or recovered, allowing on-site remediation. Barrier components may be used to in the treatment by collecting or removing contaminants or other materials from the zone of interest.

  7. Acute toxicity and aqueous solubility of some condensed thiophenes and their microbial metabolites

    SciTech Connect (OSTI)

    Seymour, D.T.; Hrudey, S.E.; Fedorak, P.M.; Verbeek, A.G.

    1997-04-01

    Petroleum or creosote contamination of surface waters, soils, or groundwaters introduces countless aromatic compounds to these environments. Among these are condensed thiophenes that were shown to be oxidized to sulfoxides, sulfones, and 2,3-diones by microbial cultures. In this study, the acute toxicities of 12 compounds (benzothiophene, benzothiophene sulfone, benzothiophene-2,3-diones, dibenzothiophene, dibenzothiophene sulfoxide, and dibenzothiophene sulfone) were determined by the Microtox{reg_sign} and Daphnia magna bioassays. To aid in determining the toxicities, the solubilities of many of these compounds were determined, which showed that the oxidized compounds were much more water soluble than the parent thiophenes. In nearly every case, the oxidized compounds were less toxic than their parent thiophenes. The Microtox method was more sensitive than the D. magna bioassay, but in general, there was a good correlation between toxicities measured by the two tests. Samples were removed from batch cultures of a Pseudomonas strain capable of oxidizing the thiophenes, and these samples were subjected to Microtox bioassays. These experiments showed that the toxicities of the culture supernatants decreased with incubation time.

  8. Old, the new, the states, the evolution of the regulation of air toxics. Master's thesis

    SciTech Connect (OSTI)

    Vecera, D.R.

    1993-02-14

    The activism associated with America in the 1960s spilled over into many areas, one of which was a new environmental movement. A product of that movement was the Clean Air Act passed in 1970. The new law included a selection aimed specifically at controlling emissions of hazardous or toxic air pollutants. However, over the next 20 years there was very little government regulation of air toxics, and this section of the Clean Air Act was considered to be a resounding failure. What went wrong. How did this lofty goal to protect human health and the environment end up on the back burner. The article will address the idealism that led to the Clean Air Act legislation, in particular the air toxics program, and explore the realities that scuttled those ideals when it came time to implement the law.

  9. In silico prediction of toxicity of non-congeneric industrial chemicals using ensemble learning based modeling approaches

    SciTech Connect (OSTI)

    Singh, Kunwar P. Gupta, Shikha

    2014-03-15

    Ensemble learning approach based decision treeboost (DTB) and decision tree forest (DTF) models are introduced in order to establish quantitative structuretoxicity relationship (QSTR) for the prediction of toxicity of 1450 diverse chemicals. Eight non-quantum mechanical molecular descriptors were derived. Structural diversity of the chemicals was evaluated using Tanimoto similarity index. Stochastic gradient boosting and bagging algorithms supplemented DTB and DTF models were constructed for classification and function optimization problems using the toxicity end-point in T. pyriformis. Special attention was drawn to prediction ability and robustness of the models, investigated both in external and 10-fold cross validation processes. In complete data, optimal DTB and DTF models rendered accuracies of 98.90%, 98.83% in two-category and 98.14%, 98.14% in four-category toxicity classifications. Both the models further yielded classification accuracies of 100% in external toxicity data of T. pyriformis. The constructed regression models (DTB and DTF) using five descriptors yielded correlation coefficients (R{sup 2}) of 0.945, 0.944 between the measured and predicted toxicities with mean squared errors (MSEs) of 0.059, and 0.064 in complete T. pyriformis data. The T. pyriformis regression models (DTB and DTF) applied to the external toxicity data sets yielded R{sup 2} and MSE values of 0.637, 0.655; 0.534, 0.507 (marine bacteria) and 0.741, 0.691; 0.155, 0.173 (algae). The results suggest for wide applicability of the inter-species models in predicting toxicity of new chemicals for regulatory purposes. These approaches provide useful strategy and robust tools in the screening of ecotoxicological risk or environmental hazard potential of chemicals. - Graphical abstract: Importance of input variables in DTB and DTF classification models for (a) two-category, and (b) four-category toxicity intervals in T. pyriformis data. Generalization and predictive abilities of the constructed (c) DTB and (d) DTF regression models to predict the T. pyriformis toxicity of diverse chemicals. - Highlights: Ensemble learning (EL) based models constructed for toxicity prediction of chemicals Predictive models used a few simple non-quantum mechanical molecular descriptors. EL-based DTB/DTF models successfully discriminated toxic and non-toxic chemicals. DTB/DTF regression models precisely predicted toxicity of chemicals in multi-species. Proposed EL based models can be used as tool to predict toxicity of new chemicals.

  10. Predictive Factors for Acute and Late Urinary Toxicity After Permanent Prostate Brachytherapy: Long-Term Outcome in 712 Consecutive Patients

    SciTech Connect (OSTI)

    Keyes, Mira Miller, Stacy; Moravan, Veronika; Pickles, Tom; McKenzie, Michael; Pai, Howard; Liu, Mitchell; Kwan, Winkle; Agranovich, Alexander; Spadinger, Ingrid; Lapointe, Vincent; Halperin, Ross; Morris, W. James

    2009-03-15

    Purpose: To describe the frequency of acute and late Radiation Therapy Oncology Group (RTOG) urinary toxicity, associated predictive factors, and resolution of International Prostate Symptom Score (IPSS) in 712 consecutive prostate brachytherapy patients. Methods and Materials: Patients underwent implantation between 1998 and 2003 (median follow-up, 57 months). The IPSS and RTOG toxicity data were prospectively collected. The patient, treatment, and implant factors were examined for an association with urinary toxicity. The time to IPSS resolution was examined using Kaplan-Meier curves, and multivariate modeling of IPSS resolution was done using Cox proportional hazards regression analysis. Logistic regression analysis was used to examine the factors associated with urinary toxicity. Results: The IPSS returned to baseline at a median of 12.6 months. On multivariate analysis, patients with a high baseline IPSS had a quicker resolution of their IPSS. Higher prostate D90 (dose covering 90% of the prostate), maximal postimplant IPSS, and urinary retention slowed the IPSS resolution time. The rate of the actuarial 5-year late urinary (>12 months) RTOG Grade 0, 1, 2, 3, and 4 was 32%, 36%, 24%, 6.2%, and 0.1%, respectively. At 7 years, the prevalence of RTOG Grade 0-1 was 92.5%. Patients with a larger prostate volume, greater number of needles, greater baseline IPSS, and use of hormonal therapy had more acute toxicity. On multivariate analysis, the significant predictors for late greater than or equal to RTOG toxicity 2 were a greater baseline IPSS, maximal postimplant IPSS, presence of acute toxicity, and higher prostate V150 (volume of the prostate covered by 150% of the dose). More recently implanted patients had less acute urinary toxicity and patients given hormonal therapy had less late urinary toxicity (all p < 0.02). Conclusion: Most urinary symptoms resolved within 12 months after prostate brachytherapy, and significant long-term urinary toxicity was very low. Refined patient selection and greater technical experience in prostate brachytherapy were associated with less urinary toxicity.

  11. Lethal body concentrations and accumulation patterns determine time-dependent toxicity of cadmium in soil arthropods

    SciTech Connect (OSTI)

    Crommentuijn, T.; Doodeman, C.J.A.M.; Doornekamp, A.; Pol, J.J.C. van der; Bedaux, J.J.M.; Gestel, C.A.M. van )

    1994-11-01

    Time-dependent toxicity in bioassays is usually explained in terms of uptake and elimination kinetics of the toxicant. By comparing different species with essentially different accumulation kinetics, a firm test of this concept may be made. This article compares the sensitivity of six soil arthropods, the collembolans Orchesella cincta and Tomocerus minor, the oribatid mite Platynothrus peltifer, the isopods Porcellio scaber and Oniscus asellus, and the diplopod Cylindroiulus britannicus, when exposed to cadmium in the food. Survival was determined at various time intervals; accumulation of cadmium in the animals was measured at one time interval. Kinetic-based toxicity models were fitted to the data, and estimates were obtained for lethal body concentration, uptake rate constant, elimination rate constant, and ultimate LC50. Two different accumulation patterns could be discerned; these were correlated with time-survival relationships. One, species that have the possibility to eliminate cadmium will reach an equilibrium for the internal concentration and also an ultimate LC50. Two, species that are unable to eliminate cadmium but store it in the body will have an ultimate LC50 equal to zero. For these species the time in which the lethal body concentration is reached is more important. Taxonomically related species appeared to have comparable accumulation patterns, but lethal body concentrations differed. It is concluded that knowledge of the accumulation pattern is indispensable for the evaluation of species' sensitivities to toxicants.

  12. Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yang, Shihui; Franden, M. A.; Brown, S. D.; Chou, Y. C.; Pienkos, P. T.; Zhang, Min

    2014-09-30

    The lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. Likewise, an understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.

  13. The risk of lead toxicity in homes with lead paint hazard

    SciTech Connect (OSTI)

    Schwartz, J.; Levin, R. )

    1991-02-01

    While lead paint has long been known to be a major source of lead poisoning, only a few small epidemiologic studies have attempted to assess directly the relative risk of lead poisoning due to the presence of lead paint. Using data from over 200,000 screening tests of children in the city of Chicago performed between 1976 and 1980, the relative risks can be quantified for children living in a major urban area. Lead paint was found to be a significant predictor of the probability of a child having lead toxicity. As expected, the reduction in leaded gasoline sales during the period reduced mean blood lead levels and increased the percentage of lead toxic children whose toxicity could be attributed to paint lead. Poisson regression models indicated that with the elimination of leaded gasoline, the relative risk of lead toxicity given lead paint exposure was 5.70 (95% CI, 4.13-7.86) during the winter and fall. The relative risk rose to 12.81 (95% CI, 7.33-22.4) in the spring and 15.8 (95% CI, 8.90-28.1) in the summer, probably due to increased exposure to window wells.

  14. Hypopharyngeal Dose Is Associated With Severe Late Toxicity in Locally Advanced Head-and-Neck Cancer: An RTOG Analysis

    SciTech Connect (OSTI)

    Machtay, Mitchell; Moughan, Jennifer; Farach, Andrew; University of Texas Health Science Center Martin-O'Meara, Elizabeth; Galvin, James; Thomas Jefferson University, Philadelphia, Pennsylvania ; Garden, Adam S.; Weber, Randal S.; Cooper, Jay S.; Forastiere, Arlene; Ang, K. Kian

    2012-11-15

    Purpose: Concurrent chemoradiation therapy (CCRT) for squamous cell carcinoma of the head and neck (SCCHN) increases local tumor control but at the expense of increased toxicity. We recently showed that several clinical/pretreatment factors were associated with the occurrence of severe late toxicity. This study evaluated the potential relationship between radiation dose delivered to the pharyngeal wall and toxicity. Methods and Materials: This was an analysis of long-term survivors from 3 previously reported Radiation Therapy Oncology Group (RTOG) trials of CCRT for locally advanced SCCHN (RTOG trials 91-11, 97-03, and 99-14). Severe late toxicity was defined in this secondary analysis as chronic grade 3-4 pharyngeal/laryngeal toxicity and/or requirement for a feeding tube {>=}2 years after registration and/or potential treatment-related death (eg, pneumonia) within 3 years. Radiation dosimetry (2-dimensional) analysis was performed centrally at RTOG headquarters to estimate doses to 4 regions of interest along the pharyngeal wall (superior oropharynx, inferior oropharynx, superior hypopharynx, and inferior hypopharynx). Case-control analysis was performed with a multivariate logistic regression model that included pretreatment and treatment potential factors. Results: A total of 154 patients were evaluable for this analysis, 71 cases (patients with severe late toxicities) and 83 controls; thus, 46% of evaluable patients had a severe late toxicity. On multivariate analysis, significant variables correlated with the development of severe late toxicity, including older age (odds ratio, 1.062 per year; P=.0021) and radiation dose received by the inferior hypopharynx (odds ratio, 1.023 per Gy; P=.016). The subgroup of patients receiving {<=}60 Gy to the inferior hypopharynx had a 40% rate of severe late toxicity compared with 56% for patients receiving >60 Gy. Oropharyngeal dose was not associated with this outcome. Conclusions: Severe late toxicity following CCRT is common in long-term survivors. Age is the most significant factor, but hypopharyngeal dose also was associated.

  15. Resistance and resilience of pond and stream ecosystems to toxicant stress: Project summary

    SciTech Connect (OSTI)

    Boston, H.L.; Stewart, A.J.; Johnson, A.R.; Bartell, S.M.

    1987-01-01

    This project will evaluate hypotheses concerning the resistance and resilience of aquatic ecosystems exposed to toxic chemicals. Our goals are to develop diagnostic criteria for ecosystem classification and to improve existing methods of ecological risk estimation. The development of models that predict ecosystem level effects requires quantifying the relationships between the underlying control structure of ecosystems (patterns of energy and material flux) and the contributions of thos structures to ecosystem resistance and resilience. We address these problems through an integration of manipulative experiments, multidimensional state space analysis, and ecosystem modeling. These studies will quantify the underlying rate structure in pond and stream systems (including, production, herbivory, nutrient uptake and recycling) and will measure changes in their structures in response to perturbations by toxicants.

  16. Attempt to estimate measurement uncertainty in the Air Force Toxic Chemical Dispersion (AFTOX) model. Master's thesis

    SciTech Connect (OSTI)

    Zettlemoyer, M.D.

    1990-01-01

    The Air Force Toxic Chemical Dispersion (AFTOX) model is a Gaussian puff dispersion model that predicts plumes, concentrations, and hazard distances of toxic chemical spills. A measurement uncertainty propagation formula derived by Freeman et al. (1986) is used within AFTOX to estimate resulting concentration uncertainties due to the effects of data input uncertainties in wind speed, spill height, emission rate, and the horizontal and vertical Gaussian dispersion parameters, and the results are compared to true uncertainties as estimated by standard deviations computed by Monte Carlo simulations. The measurement uncertainty uncertainty propagation formula was found to overestimate measurement uncertainty in AFTOX-calculated concentrations by at least 350 percent, with overestimates worsening with increasing stability and/or increasing measurement uncertainty.

  17. New Tool for Studying Toxic Metals in the Environment | U.S. DOE Office of

    Office of Science (SC) Website

    Science (SC) New Tool for Studying Toxic Metals in the Environment Biological and Environmental Research (BER) BER Home About Research Facilities Science Highlights Searchable Archive of BER Highlights External link Benefits of BER Funding Opportunities Biological & Environmental Research Advisory Committee (BERAC) Community Resources Contact Information Biological and Environmental Research U.S. Department of Energy SC-23/Germantown Building 1000 Independence Ave., SW Washington, DC

  18. Lethal and sublethal measures of chronic copper toxicity in the eastern narrowmouth toad, Gastrophryne carolinensis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LETHAL AND SUBLETHAL MEASURES OF CHRONIC COPPER TOXICITY IN THE EASTERN NARROWMOUTH TOAD, GASTROPHRYNE CAROLINENSIS R. WESLEY FLYNN,*y DAVID E. SCOTT,y WENDY KUHNE,zx DIANA SOTEROPOULOS,y and STACEY L. LANCEx ySavannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina, USA zSavannah River National Laboratory, Department of Energy, University of Georgia,, Aiken, South Carolina, USA xDepartment of Biology, University of South Carolina-Aiken, Aiken, South Carolina, USA

  19. Identifying Biomarkers and Mechanisms of Toxic Metal Stress with Global Proteomics

    SciTech Connect (OSTI)

    Miller, Susan M.

    2012-04-16

    Hg is a wide-spread contaminant in the environment and is toxic in all of its various forms. Data suggest that RHg+ and Hg2+ are toxic in two ways. At low levels, Hg species appear to disrupt membrane-bound respiration causing a burst of reactive oxygen species (ROS) that further damage the cell. At higher Hg concentrations, RHg+ and Hg2+ may form adducts with cysteine- and selenocysteine-containing proteins in all cellular compartments resulting in their inactivation. Although these mechansims for toxicity are generally accepted, the most sensitive targets associated with these mechanisms are not well understood. In this collaborative project involving three laboratories at three institutions, the overall goal was to develop of a mass spectrometry-based global proteomics methodology that could be used to identify Hg-adducted (and ideally, ROS-damaged) proteins in order to address these types of questions. The two objectives of this overall collaborative project were (1) to identify, quantify, and compare ROS- and Hg-damaged proteins in cells treated with various Hg species and concentrations to test this model for two mechanisms of Hg toxicity, and (2) to define the cellular roles of the ubiquitous bacterial mercury resistance (mer) locus with regards to how the proteins of this pathway interact to protect other cell proteins from Hg damage. The specific objectives and accomplishments of the Miller lab in this project included: (1) Development of algorithms for analysis of the Hg-proteomic mass spectrometry data to identify mercury adducted peptides and other trends in the data. (2) Investigation of the role of mer operon proteins in scavenging Hg(II) from other mer pathway proteins as a means of protecting cellular proteins from damage.

  20. Ligand-specific transcriptional mechanisms underlie aryl hydrocarbon receptor-mediated developmental toxicity of oxygenated PAHs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Goodale, B. C.; Geisel School of Medicine at Dartmouth, Hanover, NH; La Du, J.; Tilton, S. C.; Pacific Northwest National Lab.; Sullivan, C. M.; Bisson, W. H.; Waters, K. M.; Tanguay, R. L.

    2015-07-03

    Polycyclic aromatic hydrocarbons (PAHs) are priority environmental contaminants that exhibit mutagenic, carcinogenic, proinflammatory, and teratogenic properties. Oxygen-substituted PAHs (OPAHs) are formed during combustion processes and via phototoxidation and biological degradation of parent (unsubstituted) PAHs. Despite their prevalence both in contaminated industrial sites and in urban air, OPAH mechanisms of action in biological systems are relatively understudied. Like parent PAHs, OPAHs exert structure-dependent mutagenic activities and activation of the aryl hydrocarbon receptor (AHR) and cytochrome p450 metabolic pathway. Four-ring OPAHs 1,9-benz-10-anthrone (BEZO) and benz(a)anthracene-7,12-dione (7,12-B[a]AQ) cause morphological aberrations and induce markers of oxidative stress in developing zebrafish with similar potency, butmore » only 7,12-B[a]AQ induces robust Cyp1a protein expression. We investigated the role of the AHR in mediating the toxicity of BEZO and 7,12-B[a]AQ, and found that knockdown of AHR2 rescued developmental effects caused by both compounds. Using RNA-seq and molecular docking, we identified transcriptional responses that precede developmental toxicity induced via differential interaction with AHR2. Redox-homeostasis genes were affected similarly by these OPAHs, while 7,12-B[a]AQ preferentially activated phase 1 metabolism and BEZO uniquely decreased visual system genes. Analysis of biological functions and upstream regulators suggests that BEZO is a weak AHR agonist, but interacts with other transcriptional regulators to cause developmental toxicity in an AHR-dependent manner. Furthermore, identifying ligand-dependent AHR interactions and signaling pathways is essential for understanding toxicity of this class of environmentally relevant compounds.« less

  1. Predictors of Severe Acute and Late Toxicities in Patients With Localized Head-and-Neck Cancer Treated With Radiation Therapy

    SciTech Connect (OSTI)

    Meyer, Francois, E-mail: francois.meyer@chuq.qc.ca [Laval University Cancer Research Center, Centre hospitalier universitaire de Quebec - L'Hotel-Dieu de Quebec, Quebec (Canada); Fortin, Andre; Wang, Chang Shu [Radiation Therapy Department, Centre hospitalier universitaire de Quebec - L'Hotel-Dieu de Quebec, Quebec (Canada); Liu, Geoffrey [Applied Molecular Oncology, Ontario Cancer Institute/Princess Margaret Hospital, Toronto (Canada); Bairati, Isabelle [Laval University Cancer Research Center, Centre hospitalier universitaire de Quebec - L'Hotel-Dieu de Quebec, Quebec (Canada)

    2012-03-15

    Purpose: Radiation therapy (RT) causes acute and late toxicities that affect various organs and functions. In a large cohort of patients treated with RT for localized head and neck cancer (HNC), we prospectively assessed the occurrence of RT-induced acute and late toxicities and identified characteristics that predicted these toxicities. Methods and Materials: We conducted a randomized trial among 540 patients treated with RT for localized HNC to assess whether vitamin E supplementation could improve disease outcomes. Adverse effects of RT were assessed using the Radiation Therapy Oncology Group Acute Radiation Morbidity Criteria during RT and one month after RT, and the Radiation Therapy Oncology Group/European Organization for Research and Treatment of Cancer Late Radiation Morbidity Scoring Scheme at six and 12 months after RT. The most severe adverse effect among the organs/tissues was selected as an overall measure of either acute or late toxicity. Grade 3 and 4 toxicities were considered as severe. Stepwise multivariate logistic regression models were used to identify all independent predictors (p < 0.05) of acute or late toxicity and to estimate odds ratios (OR) for severe toxicity with their 95% confidence intervals (CI). Results: Grade 3 or 4 toxicity was observed in 23% and 4% of patients, respectively, for acute and late toxicity. Four independent predictors of severe acute toxicity were identified: sex (female vs. male: OR = 1.72, 95% confidence interval [CI]: 1.06-2.80), Karnofsky Performance Status (OR = 0.67 for a 10-point increment, 95% CI: 0.52-0.88), body mass index (above 25 vs. below: OR = 1.88, 95% CI: 1.22-2.90), TNM stage (Stage II vs. I: OR = 1.91, 95% CI: 1.25-2.92). Two independent predictors were found for severe late toxicity: female sex (OR = 3.96, 95% CI: 1.41-11.08) and weight loss during RT (OR = 1.26 for a 1 kg increment, 95% CI: 1.12-1.41). Conclusions: Knowledge of these predictors easily collected in a clinical setting could help tailoring therapies to reduce toxicities among patients treated with RT for HNC.

  2. Air toxics provisions of the Clean Air Act: Potential impacts on energy

    SciTech Connect (OSTI)

    Hootman, H.A.; Vernet, J.E.

    1991-11-01

    This report provides an overview of the provisions of the Clean Air Act and its Amendments of 1990 that identify hazardous air pollutant (HAP) emissions and addresses their regulation by the US Environmental Protection Agency (EPA). It defines the major energy sector sources of these HAPs that would be affected by the regulations. Attention is focused on regulations that would cover coke oven emissions; chromium emission from industrial cooling towers and the electroplating process; HAP emissions from tank vessels, asbestos-related activities, organic solvent use, and ethylene oxide sterilization; and emissions of air toxics from municipal waste combustors. The possible implications of Title III regulations for the coal, natural gas, petroleum, uranium, and electric utility industries are examined. The report discusses five major databases of HAP emissions: (1) TRI (EPA`s Toxic Release Inventory); (2) PISCES (Power Plant Integrated Systems: Chemical Emissions Studies developed by the Electric Power Research Institute); (3) 1985 Emissions Inventory on volatile organic compounds (used for the National Acid Precipitation Assessment Program); (4) Particulate Matter Species Manual (EPA); and (5) Toxics Emission Inventory (National Aeronautics and Space Administration). It also offers information on emission control technologies for municipal waste combustors.

  3. Air toxics provisions of the Clean Air Act: Potential impacts on energy

    SciTech Connect (OSTI)

    Hootman, H.A.; Vernet, J.E.

    1991-11-01

    This report provides an overview of the provisions of the Clean Air Act and its Amendments of 1990 that identify hazardous air pollutant (HAP) emissions and addresses their regulation by the US Environmental Protection Agency (EPA). It defines the major energy sector sources of these HAPs that would be affected by the regulations. Attention is focused on regulations that would cover coke oven emissions; chromium emission from industrial cooling towers and the electroplating process; HAP emissions from tank vessels, asbestos-related activities, organic solvent use, and ethylene oxide sterilization; and emissions of air toxics from municipal waste combustors. The possible implications of Title III regulations for the coal, natural gas, petroleum, uranium, and electric utility industries are examined. The report discusses five major databases of HAP emissions: (1) TRI (EPA's Toxic Release Inventory); (2) PISCES (Power Plant Integrated Systems: Chemical Emissions Studies developed by the Electric Power Research Institute); (3) 1985 Emissions Inventory on volatile organic compounds (used for the National Acid Precipitation Assessment Program); (4) Particulate Matter Species Manual (EPA); and (5) Toxics Emission Inventory (National Aeronautics and Space Administration). It also offers information on emission control technologies for municipal waste combustors.

  4. Nano/bio treatment of polychlorinated biphenyls with evaluation of comparative toxicity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Le, Thao Thanh; Francis, Arokiasamy J.; Nguyen, Hoang Khanh; Jeon, Jong -Rok; Chang, Yoon -Seok

    2015-02-03

    The persistence of polychlorinated biphenyl (PCB) Aroclor 1248 in soils and sediments is a major concern because of its toxicity and presence at high concentrations. In this study, we developed an integrated remediation system for PCBs using chemical catalysis and biodegradation. The dechlorination of Aroclor 1248 was achieved by treatment with bimetallic nanoparticles Pd/nFe under anoxic conditions. Among the 32 PCB congeners of Aroclor 1248 examined, our process dechlorinated 99%, 92%, 84%, and 28% of tri-, tetra-, penta-, and hexachlorinated biphenyls, respectively. The resulting biphenyl was biodegraded rapidly by Burkholderia xenovorans LB400. Benzoic acid was detected as an intermediate duringmore » the biodegradation process. The toxicity of the residual PCBs after nano-bio treatment was evaluated in terms of toxic equivalent values which decreased from 33.8 × 10-5 μg g-1 to 9.5 × 10-5 μg g-1. The residual PCBs also had low cytotoxicity toward Escherichia coli as demonstrated by lower reactive oxygen species levels, lower glutathione peroxidase activity, and a reduced number of dead bacteria.« less

  5. Inhalation developmental toxicology studies: Developmental toxicity of chloroprene vapors in New Zealand white rabbits. Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Evanoff, J.J.; Westerberg, R.B.; Rommereim, R.L.; Weigel, R.J.

    1994-04-01

    Chloroprene, 2-chloro-1,3-butadiene, is a colorless liquid with a pungent ethereal odor that is primarily used as an intermediate in the manufacture of neoprene rubber, and has been used as such since about 1930. This study addressed the potential for chloroprene to cause developmental toxicity in New Zealand white rabbits following gestational exposure to 0, 10, 40, or 175 ppm chloroprene vapors, 6h/dy, 7dy/wk. Each treatment group consisted of 15 artificially inseminated females exposed on 6 through 28 days of gestation (dg). Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice on 29 dg. Implants were enumerated and their status recorded and live fetuses were examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. There were no overt signs of maternal toxicity and the change in maternal body weight over the course of the study was not affected. Exposure of pregnant rabbits to chloroprene vapors on 6-28 dg had no effect on the number of implantation, the mean percent of live pups per litter, or on the incidence of resorptions per litter. The incidence of fetal malformations was not increased by exposure to chloroprene. Results of this study indicate that gestational exposure of New Zealand white rabbits to 10, 40, or 175 ppm chloroprene did not result in observable toxicity to either the dam or the offspring.

  6. Quality of Life and Toxicity From Passively Scattered and Spot-Scanning Proton Beam Therapy for Localized Prostate Cancer

    SciTech Connect (OSTI)

    Pugh, Thomas J. [Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Munsell, Mark F. [Department of Biostatistics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Choi, Seungtaek; Nguyen, Quyhn Nhu; Mathai, Benson [Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Zhu, X. Ron; Sahoo, Narayan; Gillin, Michael; Johnson, Jennifer L.; Amos, Richard A. [Department of Radiation Physics, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Dong, Lei [Scripps Proton Therapy Center, San Diego, California (United States); Mahmood, Usama; Kuban, Deborah A.; Frank, Steven J.; Hoffman, Karen E.; McGuire, Sean E. [Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Lee, Andrew K., E-mail: aklee@mdanderson.org [Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas (United States)

    2013-12-01

    Purpose: To report quality of life (QOL)/toxicity in men treated with proton beam therapy for localized prostate cancer and to compare outcomes between passively scattered proton therapy (PSPT) and spot-scanning proton therapy (SSPT). Methods and Materials: Men with localized prostate cancer enrolled on a prospective QOL protocol with a minimum of 2 years' follow-up were reviewed. Comparative groups were defined by technique (PSPT vs SSPT). Patients completed Expanded Prostate Cancer Index Composite questionnaires at baseline and every 3-6 months after proton beam therapy. Clinically meaningful differences in QOL were defined as ?0.5 baseline standard deviation. The cumulative incidence of modified Radiation Therapy Oncology Group grade ?2 gastrointestinal (GI) or genitourinary (GU) toxicity and argon plasma coagulation were determined by the Kaplan-Meier method. Results: A total of 226 men received PSPT, and 65 received SSPT. Both PSPT and SSPT resulted in statistically significant changes in sexual, urinary, and bowel Expanded Prostate Cancer Index Composite summary scores. Only bowel summary, function, and bother resulted in clinically meaningful decrements beyond treatment completion. The decrement in bowel QOL persisted through 24-month follow-up. Cumulative grade ?2 GU and GI toxicity at 24 months were 13.4% and 9.6%, respectively. There was 1 grade 3 GI toxicity (PSPT group) and no other grade ?3 GI or GU toxicity. Argon plasma coagulation application was infrequent (PSPT 4.4% vs SSPT 1.5%; P=.21). No statistically significant differences were appreciated between PSPT and SSPT regarding toxicity or QOL. Conclusion: Both PSPT and SSPT confer low rates of grade ?2 GI or GU toxicity, with preservation of meaningful sexual and urinary QOL at 24 months. A modest, yet clinically meaningful, decrement in bowel QOL was seen throughout follow-up. No toxicity or QOL differences between PSPT and SSPT were identified. Long-term comparative results in a larger patient cohort are warranted.

  7. WE-D-BRE-03: Late Toxicity Following Photon Or Proton Radiotherapy in Patients with Brain Tumors

    SciTech Connect (OSTI)

    Munbodh, R; Ding, X; Yin, L; Anamalayil, S; Dorsey, J; Lustig, R; Alonso-Basanta, M

    2014-06-15

    Purpose: To identify indicators of Late Grade 3 (LG3) toxicity, late vision and hearing changes in patients treated for primary brain tumors with photon (XRT) or proton radiotherapy (PRT). Methods: We retrospectively reviewed 102 patients who received brain XRT or PRT to doses of 54 or 59.6 Gy in daily fractions of 1.8–2 Gy. Of the 80 patients (34 XRT, 39 PRT and 7 both modalities) reviewed for indicators of LG3 toxicity, 25 developed LG3 toxicity 90 to 500 days after radiotherapy completion. 55 patients had less than LG3 toxicity > 500 days after treatment. In that time, late vision and hearing changes were seen in 44 of 75 and 25 of 78 patients, respectively. The correlation between late toxicity and prescription dose, planning target volume (PTV) size, and doses to the brainstem, brain, optic chiasm, optic nerves, eyes and cochlea was evaluated. A two-tailed Fisher's exact test and Wilcoxon rank sum test were used for the statistical analysis for XRT, PRT and all patients combined. Results: Exceeding the 54 Gy-5% dose-volume brainstem constraint, but not the optic structure constraints, was significantly correlated (p < 0.05) with late vision changes in all three groups. Exceeding maximum and mean cochlear doses of 45 and 30 Gy, respectively, was a significant indicator of hearing changes (p < 0.05) in PRT patients and all patients combined. In a sub-group of 52 patients in whom the brain was contoured, the absolute brain volume receiving ≤ 50 Gy and > 60 Gy was significantly larger in patients with LG3 toxicity for all patients combined (p < 0.05). Prescription dose, brainstem dose and PTV volume were not correlated to LG3 toxicity. Conclusion: Our results indicate the importance of minimizing the brain volume irradiated, and brainstem and cochlea doses to reduce the risk of late toxicities following brain radiotherapy.

  8. CHALLENGES WITH RETRIEVING TRANSURANIC WASTE FROM THE HANFORD BURIAL GROUNDS

    SciTech Connect (OSTI)

    SWAN, R.J.; LAKES, M.E.

    2007-08-06

    The U.S. DOE's Hanford Reservation produced plutonium and other nuclear materials for the nation's defense starting in World War II. The defense mission generated wastes that were either retrievably stored (i.e. retrievably stored waste) and/or disposed of in burial grounds. Challenges have emerged from retrieving suspect TRU waste including adequacy of records, radiological concerns, container integrity, industrial hygiene and safety issues, the lack of processing/treatment facilities, and the integration of regulatory requirements. All retrievably stored waste is managed as mixed waste and assumed to be TRU waste, unless documented otherwise. Mixed waste is defined as radioactive waste that contains hazardous constituents. The Atomic Energy Act governs waste with radionuclides, and the Resource Conservation and Recovery Act (RCRA) governs waste with hazardous constituents. Waste may also be governed by the Toxic Substances Control Act (TSCA), and a portion may be managed under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA). In 1970, TRU waste was required to be placed in 20-year retrievable storage and segregated from other Waste. Prior to that date, segregation did not occur. Because of the changing definition of TRU over the years, and the limitations of early assay equipment, all retrievably stored waste in the burial grounds is managed as suspect TRU. Experience has shown that some of this waste will be characterized as low-level (non-TRU) waste after assay. The majority of the retrieved waste is not amenable to sampling due to waste type and/or radiological issues. Key to waste retrieval and disposition are characterization, historical investigation and research, knowledge of past handling and packaging, as well as a broad understanding and application of the regulations.

  9. Migration and Retardation of Chemical Toxic Components from Radioactive Waste - Hydrochemical Aspects

    SciTech Connect (OSTI)

    Jedinakova-Krizova, V.; Hanslik, E.

    2003-02-24

    A systematic analysis of nuclear power plant (NPP) operation and radioactive wastes disposal (near-surface disposal and geologic disposal) in underground repositories has provided the basis for a comparison between the radiotoxicity and chemotoxicity as part of an EIA (environmental impact assessment) procedure. This contribution summarizes the hydrochemical mechanisms of transport and retardation processes, chemistry and migration behavior of radionuclides and chemical toxics in natural sorbents, especially bentonites. The effect of solubility and dissolution reactions, diffusion and sorption/desorption, complexation and variations in the aqueous phase composition, pH-value and oxidation-reduction properties and other phenomena affecting distribution coefficients (Kd values) is discussed.

  10. Acute environmental toxicity and persistence of methyl salicylate: A chemical agent simulant. Final report

    SciTech Connect (OSTI)

    Cataldo, D.A.; Ligotke, M.W.; Harvey, S.D.; Fellows, R.J.; Li, S.W.

    1994-06-01

    The interactions of methyl salicylate with plant foliage and soils were assessed using aerosol/vapor exposure methods. Measurements of deposition velocity and residence times for soils and foliar surfaces are reported. Severe plant contact toxicity was observed at foliar mass-loading levels above 4 {mu}g/cm{sup 2} leaf; however, recovery was noted after four to fourteen days. Methyl salicylate has a short-term effect on soil dehydrogenase activity, but not phosphatase activity. Results of the earthworm bioassay indicated only minimal effects on survival.

  11. Molecular Mimicry of SUMO by Rad60 Helps Protect the Genome from Toxic

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Stress Molecular Mimicry of SUMO by Rad60 Helps Protect the Genome from Toxic Stress DNA is subject to multiple forms of damage that can occur either spontaneously, through sources such as reactive oxygen species produced by cellular metabolism, or through exogenous sources such as UV light and X-rays. Different cellular pathways exist to detect and repair the distinct forms of damage, and thus maintain the stability of the genome. The importance of the DNA repair pathways is highlighted by

  12. Portsmouth TSCA UE FFCA 02-20-1992 Compliance Agreement Modification 07-17-97_1.pdf

    Energy Savers [EERE]

  13. Hazardous Substance Release Reporting Under CERCLA, EPCR {section}304 and DOE Emergency Management System (EMS) and DOE Occurrence Reporting Requirements. Environmental Guidance

    SciTech Connect (OSTI)

    Traceski, T.T.

    1994-06-01

    Releases of various substances from DOE facilities may be subject to reporting requirements under the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and the Emergency Planning and Community Right-to-Know Act (EPCRA), as well as DOE`s internal ``Occurrence Reporting and Processing of Operations Information`` and the ``Emergency Management System`` (EMS). CERCLA and EPCPA are Federal laws that require immediate reporting of a release of a Hazardous Substance (HS) and an Extremely Hazardous Substance (EHS), respectively, in a Reportable Quantity (RQ) or more within a 24-hour period. This guidance uses a flowchart, supplemental information, and tables to provide an overview of the process to be followed, and more detailed explanations of the actions that must be performed, when chemical releases of HSs, EHSs, pollutants, or contaminants occur at DOE facilities. This guidance should be used in conjunction with, rather than in lieu of, applicable laws, regulations, and DOE Orders. Relevant laws, regulations, and DOE Orders are referenced throughout this guidance.

  14. Control of Toxic Chemicals in Puget Sound, Phase 3: Study of Atmospheric Deposition of Air Toxics to the Surface of Puget Sound

    SciTech Connect (OSTI)

    Brandenberger, Jill M.; Louchouarn, Patrick; Kuo, Li-Jung; Crecelius, Eric A.; Cullinan, Valerie I.; Gill, Gary A.; Garland, Charity R.; Williamson, J. B.; Dhammapala, R.

    2010-07-05

    The results of the Phase 1 Toxics Loading study suggested that runoff from the land surface and atmospheric deposition directly to marine waters have resulted in considerable loads of contaminants to Puget Sound (Hart Crowser et al. 2007). The limited data available for atmospheric deposition fluxes throughout Puget Sound was recognized as a significant data gap. Therefore, this study provided more recent or first reported atmospheric deposition fluxes of PAHs, PBDEs, and select trace elements for Puget Sound. Samples representing bulk atmospheric deposition were collected during 2008 and 2009 at seven stations around Puget Sound spanning from Padilla Bay south to Nisqually River including Hood Canal and the Straits of Juan de Fuca. Revised annual loading estimates for atmospheric deposition to the waters of Puget Sound were calculated for each of the toxics and demonstrated an overall decrease in the atmospheric loading estimates except for polybrominated diphenyl ethers (PBDEs) and total mercury (THg). The median atmospheric deposition flux of total PBDE (7.0 ng/m2/d) was higher than that of the Hart Crowser (2007) Phase 1 estimate (2.0 ng/m2/d). The THg was not significantly different from the original estimates. The median atmospheric deposition flux for pyrogenic PAHs (34.2 ng/m2/d; without TCB) shows a relatively narrow range across all stations (interquartile range: 21.2- 61.1 ng/m2/d) and shows no influence of season. The highest median fluxes for all parameters were measured at the industrial location in Tacoma and the lowest were recorded at the rural sites in Hood Canal and Sequim Bay. Finally, a semi-quantitative apportionment study permitted a first-order characterization of source inputs to the atmosphere of the Puget Sound. Both biomarker ratios and a principal component analysis confirmed regional data from the Puget Sound and Straits of Georgia region and pointed to the predominance of biomass and fossil fuel (mostly liquid petroleum products such as gasoline and/or diesel) combustion as source inputs of combustion by-products to the atmosphere of the region and subsequently to the waters of Puget Sound.

  15. Determination of the toxicity characteristic for metals in soil: A comparison of the toxicity characteristic leaching procedure and total metal determination

    SciTech Connect (OSTI)

    Bass, D.A.; Taylor, J.D.

    1994-12-01

    A comparison is made of the concentrations of metals extracted from soils using the Toxicity Characteristic Leaching Procedure (TCLP) and a total determination method. This information is of interest in two ways. First, it is hoped that a relationship might be established between the amount of each metal determined after extraction by the TCLP and the amount determined using a total determination method. And second, data are also presented which indicate the general extractability of various metals in soil samples using the TCLP. This study looks specifically at inorganic elements (Sb, As, Ba, Cd, Cu, Cr, Pb, Mg, Hg, Se, Ag, Sn, and Zn) in soils from a firing range. Results show that total determination methods for metals can not generally be used for heterogeneous samples, such as soil samples from a firing range. Some correlation between a total determination method and TCLP was observed when Ba and Cd were present in the samples at lower concentrations (less than 80 mg/kg for Ba and less than 25 mg/kg for Cd); however, additional data are necessary to verify this correlation.

  16. Hyperfractionated Accelerated Radiotherapy (HART) for Anaplastic Thyroid Carcinoma: Toxicity and Survival Analysis

    SciTech Connect (OSTI)

    Dandekar, Prasad; Rhys-Evans, Peter; Harrington, Kevin; Nutting, Christopher; Newbold, Kate

    2009-06-01

    Purpose: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive cancers, and the current protocol of hyperfractionated accelerated radiotherapy was initiated to improve survival while limiting toxicities. Methods and Materials: All patients with ATC from 1991 to 2002 were accrued and received megavoltage radiotherapy from the mastoid processes to the carina up to 60 Gy in twice-daily fractions of 1.8 and 2 Gy, 6 hours apart. Results: Thirty-one patients were accrued with a median age of 69 years, and 55% were women. Debulking was performed in 26%, and total thyroidectomy, in 6%, whereas 68% received radical radiotherapy alone. Local control data were available for 27 patients: 22% had a complete response, 26% had a partial response, 15% showed progressive disease, and 37% showed static disease. Median overall survival for all 31 patients was 70 days (95% confidence interval, 40-99). There was no significant difference in median survival between patients younger (70 days) and older than 70 years (42 days), between men (70 days) and women (49days), and between patients receiving postoperative radiotherapy (77 days) and radical radiotherapy alone (35 days). Grade III or higher skin erythema was seen in 56% patients; desquamation in 21%; dysphagia in 74%; and esophagitis in 79%. Conclusion: The current protocol failed to offer a significant survival benefit, was associated with severe toxicities, and thus was discontinued. There is a suggestion that younger patients with operable disease have longer survival, but this would require a larger study to confirm it.

  17. Toxicity of Select Organic Acids to the Slightly Thermophilic Acidophile Acidithiobaccillus Caldus

    SciTech Connect (OSTI)

    John E Aston; William A Apel; Brady D Lee; Brent M Peyton

    2009-02-01

    Acidithiobacillus caldus is a thermophilic acidophile found in commercial biomining, acid mine drainage systems, and natural environments. Previous work has characterized A. caldus as a chemolithotrophic autotroph capable of utilizing reduced sulfur compounds under aerobic conditions. Organic acids are especially toxic to chemolithotrophs in low-pH environments, where they diffuse more readily into the cell and deprotonate within the cytoplasm. In the present study, the toxic effects of oxaloacetate, pyruvate, 2-ketoglutarate, acetate, malate, succinate, and fumarate on A. caldus strain BC13 were examined under batch conditions. All tested organic acids exhibited some inhibitory effect. Oxaloacetate was observed to inhibit growth completely at a concentration of 250 M, whereas other organic acids were completely inhibitory at concentrations of between 1,000 and 5,000 M. In these experiments, the measured concentrations of organic acids decreased with time, indicating uptake or assimilation by the cells. Phospholipid fatty acid analyses indicated an effect of organic acids on the cellular envelope. Notable differences included an increase in cyclic fatty acids in the presence of organic acids, indicating possible instability of the cellular envelope. This was supported by field emission scanning-electron micrographs showing blebbing and sluffing in cells grown in the presence of organic acids.

  18. ABB`s investigations into air toxic emissions from fossil fuel and MSW combustion

    SciTech Connect (OSTI)

    Wesnor, J.D.

    1994-12-31

    Since passage of the Clean Air Act, Asea Brown Boveri (ABB) has been actively developing a knowledge base on the Title 3 hazardous air pollutants, more commonly called air toxics. As ABB is a multinational company, US operating companies are able to call upon work performed by European counterparts, who have faced similar legislation several years ago. In addition to the design experience and database acquired in Europe, ABB Inc. has been pursuing several other avenues to expand its air toxics knowledge. ABB Combustion Engineering (ABB CE) is presently studying the formation of organic pollutants within the combustion furnace and partitioning of trace metals among the furnace outlet streams. ABB Environmental Systems (ABBES) has reviewed available and near-term control technologies and methods. Also, both ABB CE and ABBES have conducted source sampling and analysis at commercial installations for hazardous air pollutants to determine the emission rates and removal performance of various types of equipment. Several different plants hosted these activities, allowing for variation in fuel type and composition, boiler configuration, and air pollution control equipment. This paper discusses the results of these investigations.

  19. Mechanisms of carbon nanotube-induced toxicity: Focus on oxidative stress

    SciTech Connect (OSTI)

    Shvedova, Anna A.; Pietroiusti, Antonio; Fadeel, Bengt; Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA ; Kagan, Valerian E.

    2012-06-01

    Nanotechnologies are emerging as highly promising technologies in many sectors in the society. However, the increasing use of engineered nanomaterials also raises concerns about inadvertent exposure to these materials and the potential for adverse effects on human health and the environment. Despite several years of intensive investigations, a common paradigm for the understanding of nanoparticle-induced toxicity remains to be firmly established. Here, the so-called oxidative stress paradigm is scrutinized. Does oxidative stress represent a secondary event resulting inevitably from disruption of biochemical processes and the demise of the cell, or a specific, non-random event that plays a role in the induction of cellular damage e.g. apoptosis? The answer to this question will have important ramifications for the development of strategies for mitigation of adverse effects of nanoparticles. Recent examples of global lipidomics studies of nanoparticle-induced tissue damage are discussed along with proteomics and transcriptomics approaches to achieve a comprehensive understanding of the complex and interrelated molecular changes in cells and tissues exposed to nanoparticles. We also discuss instances of non-oxidative stress-mediated cellular damage resulting from direct physical interference of nanomaterials with cellular structures. -- Highlights: ? CNT induced non-random oxidative stress associated with apoptosis. ? Non-oxidative mechanisms for cellular toxicity of carbon nanotubes. ? Biodegradation of CNT by cells of innate immune system. ? Omics-based biomarkers of CNT exposures.

  20. Structure-toxicity assessment of metabolites of the aerobic bacterial transformation of substituted naphthalenes

    SciTech Connect (OSTI)

    LeBlond, J.D.; Applegate, B.M.; Menn, F.M.; Schultz, T.W.; Sayler, G.S.

    2000-05-01

    Pseudomonas fluorescens 5R, a naphthalene-degrading bacterium isolated from manufactured gas plant soil contaminated with polycyclic aromatic hydrocarbons, was examined for its degradative capacity of a number of substituted naphthalenes. In general, those compounds substituted on only one ring with an electrically neutral substituent were found to be transformed primarily to substituted salicylic acids according to the classical (NAH7) naphthalene dioxygenase-initiated upper pathway reactions of the naphthalene degradative pathway (i.e., the NAH system). Dimethylnaphthalenes with a substituent on each ring, and certain halogenated naphthalenes, were transformed via a monohydroxylation reaction to form hydroxylated dead-end products. Of the substituted salicylic acids examined, only 3- and 4-methylsalicylic acid, the respective products of the degradation of 1- and 2-methylnaphthalene, were further degraded by salicylate hydroxylase and catechol 2,3-dioxygenase, the first two enzymes of the NAH lower pathway. Using the Tetrahymena pyriformis acute toxicity assay, many of the monohydroxylated products of incomplete biodegradation were found to be polar narcotics. Substituted salicylic acids that are not further degraded by the NAH lower pathway were found to be toxic via carboxylic acid narcosis.

  1. Small heat shock proteins protect against {alpha}-synuclein-induced toxicity and aggregation

    SciTech Connect (OSTI)

    Outeiro, Tiago Fleming [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); Klucken, Jochen [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); Strathearn, Katherine E. [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-2091 (United States); Liu Fang [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-2091 (United States); Nguyen, Paul [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); Rochet, Jean-Christophe [Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-2091 (United States); Hyman, Bradley T. [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States); McLean, Pamela J. [Alzheimer's Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH, Harvard Medical School, CNY 114, 16th Street, Charlestown, MA 02129 (United States)]. E-mail: touteiro@partners.org

    2006-12-22

    Protein misfolding and inclusion formation are common events in neurodegenerative diseases, such as Parkinson's disease (PD), Alzheimer's disease (AD) or Huntington's disease (HD). {alpha}-Synuclein (aSyn) is the main protein component of inclusions called Lewy bodies (LB) which are pathognomic of PD, Dementia with Lewy bodies (DLB), and other diseases collectively known as LB diseases. Heat shock proteins (HSPs) are one class of the cellular quality control system that mediate protein folding, remodeling, and even disaggregation. Here, we investigated the role of the small heat shock proteins Hsp27 and {alpha}B-crystallin, in LB diseases. We demonstrate, via quantitative PCR, that Hsp27 messenger RNA levels are {approx}2-3-fold higher in DLB cases compared to control. We also show a corresponding increase in Hsp27 protein levels. Furthermore, we found that Hsp27 reduces aSyn-induced toxicity by {approx}80% in a culture model while {alpha}B-crystallin reduces toxicity by {approx}20%. In addition, intracellular inclusions were immunopositive for endogenous Hsp27, and overexpression of this protein reduced aSyn aggregation in a cell culture model.

  2. Chronomodulation of topotecan or X-radiation treatment increases treatment efficacy without enhancing acute toxicity

    SciTech Connect (OSTI)

    Mullins, Dana; Proulx, Denise; Saoudi, A.; Ng, Cheng E. . E-mail: cng@ohri.ca

    2005-05-01

    Purpose: Topotecan (TPT), a camptothecin analog, is currently used to treat human ovarian and small-cell lung cancer and is in clinical trials for other tumor sites. However, it is unknown whether chronomodulation of TPT treatment is beneficial. We examined the effects of administering TPT or X-radiation (XR) alone at different times of the day or night. Methods: We treated mice bearing human colorectal tumor xenografts at four different times representing the early rest period (9 AM or 3 HALO [hours after light onset]), late rest period (3 PM or 9 HALO), early active period (9 PM or 15 HALO), and late active period (3 AM or 21 HALO) of the mice. We gave either TPT (12 mg/kg, injected i.p.) or XR (4 Gy, directed to the tumor) twice weekly on Days 0, 4, 7, 10 within 2 weeks. Results: Treatment with either TPT or XR at 3 AM demonstrated the greatest efficacy (measured by a tumor regrowth assay) without significantly increasing acute toxicity (assessed by a decrease in leukocyte counts or body weight). Conversely, treatment at 3 PM, in particular, showed increased toxicity without any enhanced efficacy. Conclusions: Our study provided the first evidence that chronomodulation of TPT treatments, consistent with the findings of other camptothecin analogs, is potentially clinically beneficial. Additionally, our findings suggest that chronomodulation of fractionated XR treatments is also potentially clinically beneficial.

  3. Comparative toxicity of pentachlorophenol to three earthworm species in artificial soil

    SciTech Connect (OSTI)

    Fitzgerald, D.; Lanno, R.P.; Farwell, A.; Dixon, D.G.

    1994-12-31

    Although methods for standardized toxicity tests with earthworms exist, many of the test parameters and conditions have not been validated in actual tests and with different species of worms. This study evaluated the toxicity of pentachlorophenol (PCP) to three species of earthworms, Lumbricus terrestris, Eisenia fetida, and Eudrilus eugeniae using various methods of data analysis and body residues. Tests were conducted in artificial soil for a period of 28 days or until an Acute Lethality Threshold (ALT) was reached. An intensive temporal sampling regime was applied to generate sufficient data for the accurate estimation of ALTs using both LC50/time and time-to-death/soil concentration methods of data analysis. L. terrestris was tested at 15 C, E. eugeniae at 24 C, and E. fetida at both temperatures. Total body residues of PCP were measured by GC following cryogenic separation of the lipid fraction of the worm. ALTs were significantly different between E. fetida and the two larger species of worms. No effect of temperature on the ALT for E. fetida was observed, although the time taken to reach the ALT increased at the lower temperature. The relationship of PCP residues at mortality will be discussed in terms of the effects of species, body size and temperature. Limitations of the artificial soil based upon growth curves of worms will also be examined.

  4. Evaluation of the effects of coal fly ash amendments on the toxicity of a contaminated marine sediment

    SciTech Connect (OSTI)

    Burgess, R.M.; Perron, M.M.; Friedman, C.L.; Suuberg, E.M.; Pennell, K.G.; Cantwell, M.G.; Pelletier, M.C.; Ho, K.T.; Serbst, J.R.; Ryba, S.A.

    2009-01-15

    Approaches for cleaning up contaminated sediments range from dredging to in situ treatment. In this study, we discuss the effects of amending reference and contaminated sediments with coal fly ash to reduce the bioavailability and toxicity of a field sediment contaminated with polycyclic aromatic hydrocarbons (PAHs). Six fly ashes and a coconut charcoal were evaluated in 7-d whole sediment toxicity tests with a marine amphipod (Ampelisca abdita) and mysid (Americamysis bahia). Fly ashes with high carbon content and the coconut charcoal showed proficiency at reducing toxicity. Some of the fly ashes demonstrated toxicity in the reference treatments. It is suspected that some of this toxicity is related to the presence of ammonia associated with fly ashes as a result of postoxidation treatment to reduce nitrous oxide emissions. Relatively simple methods exist to remove ammonia from fly ash before use, and fly ashes with low ammonia content are available. Fly ashes were also shown to effectively reduce overlying water concentrations of several PAHs. No evidence was seen of the release of the metals cadmium, copper, nickel, or lead from the fly ashes. A preliminary 28-d polychaete bioaccumulation study with one of the high-carbon fly ashes and a reference sediment was also performed. Although preliminary, no evidence was seen of adverse effects to worm growth or lipid content or of accumulation of PAHs or mercury from exposure to the fly ash. These data show fly ashes with high carbon content could represent viable remedial materials for reducing the bioavailability of organic contaminants in sediments.

  5. Comparative hazard analysis and toxicological modeling of diverse nanomaterials using the embryonic zebrafish (EZ) metric of toxicity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Harper, Bryan; Thomas, Dennis G.; Chikkagoudar, Satish; Baker, Nathan A.; Tang, Kaizhi; Heredia-Langner, Alejandro; Lins, Roberto D.; Harper, Stacey

    2015-06-04

    The integration of rapid assays, large data sets, informatics and modeling can overcome current barriers in understanding nanomaterial structure-toxicity relationships by providing a weight-of-the-evidence mechanism to generate hazard rankings for nanomaterials. Here we present the use of a rapid, low-cost assay to perform screening-level toxicity evaluations of nanomaterials in vivo. Calculated EZ Metric scores, a combined measure of morbidity and mortality, were established at realistic exposure levels and used to develop a predictive model of nanomaterial toxicity. Hazard ranking and clustering analysis of 68 diverse nanomaterials revealed distinct patterns of toxicity related to both core composition and outermost surface chemistrymore » of nanomaterials. The resulting clusters guided the development of a predictive model of gold nanoparticle toxicity to embryonic zebrafish. In addition, our findings suggest that risk assessments based on the size and core composition of nanomaterials alone may be wholly inappropriate, especially when considering complex engineered nanomaterials. These findings reveal the need to expeditiously increase the availability of quantitative measures of nanomaterial hazard and broaden the sharing of that data and knowledge to support predictive modeling. In addition, research should continue to focus on methodologies for developing predictive models of nanomaterial hazard based on sub-lethal responses to low dose exposures.« less

  6. Comparative hazard analysis and toxicological modeling of diverse nanomaterials using the embryonic zebrafish (EZ) metric of toxicity

    SciTech Connect (OSTI)

    Harper, Bryan; Thomas, Dennis G.; Chikkagoudar, Satish; Baker, Nathan A.; Tang, Kaizhi; Heredia-Langner, Alejandro; Lins, Roberto D.; Harper, Stacey

    2015-06-04

    The integration of rapid assays, large data sets, informatics and modeling can overcome current barriers in understanding nanomaterial structure-toxicity relationships by providing a weight-of-the-evidence mechanism to generate hazard rankings for nanomaterials. Here we present the use of a rapid, low-cost assay to perform screening-level toxicity evaluations of nanomaterials in vivo. Calculated EZ Metric scores, a combined measure of morbidity and mortality, were established at realistic exposure levels and used to develop a predictive model of nanomaterial toxicity. Hazard ranking and clustering analysis of 68 diverse nanomaterials revealed distinct patterns of toxicity related to both core composition and outermost surface chemistry of nanomaterials. The resulting clusters guided the development of a predictive model of gold nanoparticle toxicity to embryonic zebrafish. In addition, our findings suggest that risk assessments based on the size and core composition of nanomaterials alone may be wholly inappropriate, especially when considering complex engineered nanomaterials. These findings reveal the need to expeditiously increase the availability of quantitative measures of nanomaterial hazard and broaden the sharing of that data and knowledge to support predictive modeling. In addition, research should continue to focus on methodologies for developing predictive models of nanomaterial hazard based on sub-lethal responses to low dose exposures.

  7. Toxicity of polychlorinated diphenyl ethers in hydra attenuata and in rat whole-embryo culture. Master's thesis

    SciTech Connect (OSTI)

    Becker, M.C.

    1991-05-01

    Polychlorinated diphenyl ethers (PCDEs) are a class of biaryl compounds that have little commercial application, but appear to be widespread in the environment. They have been found in wood preservative waste dumpsites and in fly ash from municipal waste incinerators. They have been detected in bird eggs and tissues, fish, and other edible marine organisms in the United States, Canada, and Europe. There are limited reports in the extant literature on the toxicity of PCDEs. This study was designed to evaluate the toxicity of selected PCDEs in cultures of Hydra attenuata and post-implantation rat whole embryos. The toxicity of several closely related polychlorinated biphenyls (PCBs) was evaluated in both cultures and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was evaluated in whole embryo culture. Embryonic growth and development parameters (yolk sac diameter, crown-rump length, somite count, and DNA and protein content) and gross morphology were determined. Findings indicated that these chemicals were neither embryotoxic nor teratogenic. Thus, the PCDEs, which elicit other diverse toxic and biochemical responses in rodents, are relatively inactive in these bioassays for developmental toxicity.

  8. Intensity-Modulated Radiotherapy as Primary Therapy for Prostate Cancer: Report on Acute Toxicity After Dose Escalation With Simultaneous Integrated Boost to Intraprostatic Lesion

    SciTech Connect (OSTI)

    Fonteyne, Valerie Villeirs, Geert; Speleers, Bruno; Neve, Wilfried de; Wagter, Carlos de; Lumen, Nicolas; Meerleer, Gert de

    2008-11-01

    Purpose: To report on the acute toxicity of a third escalation level using intensity-modulated radiotherapy for prostate cancer (PCa) and the acute toxicity resulting from delivery of a simultaneous integrated boost (SIB) to an intraprostatic lesion (IPL) detected on magnetic resonance imaging (MRI), with or without spectroscopy. Methods and Materials: Between January 2002 and March 2007, we treated 230 patients with intensity-modulated radiotherapy to a third escalation level as primary therapy for prostate cancer. If an IPL (defined by MRI or MRI plus spectroscopy) was present, a SIB was delivered to the IPL. To report on acute toxicity, patients were seen weekly during treatment and 1 and 3 months after treatment. Toxicity was scored using the Radiation Therapy Oncology Group toxicity scale, supplemented by an in-house-developed scoring system. Results: The median dose to the planning target volume was 78 Gy. An IPL was found in 118 patients. The median dose to the MRI-detected IPL and MRI plus spectroscopy-detected IPL was 81 Gy and 82 Gy, respectively. No Grade 3 or 4 acute gastrointestinal toxicity developed. Grade 2 acute gastrointestinal toxicity was present in 26 patients (11%). Grade 3 genitourinary toxicity was present in 15 patients (7%), and 95 patients developed Grade 2 acute genitourinary toxicity (41%). No statistically significant increase was found in Grade 2-3 acute gastrointestinal or genitourinary toxicity after a SIB to an IPL. Conclusion: The results of our study have shown that treatment-induced acute toxicity remains low when intensity-modulated radiotherapy to 80 Gy as primary therapy for prostate cancer is used. In addition, a SIB to an IPL did not increase the severity or incidence of acute toxicity.

  9. Comparing Metal Leaching and Toxicity from High pH, Low pH, and High Ammonia Fly Ash

    SciTech Connect (OSTI)

    Palumbo, Anthony Vito; Phillips, Jana Randolph; Fagan, Lisa Anne; Drake, Meghan M; Ruther, Rose Emily; Fisher, L. Suzanne; Amonette, J. E.

    2007-01-01

    Previous work with both class F and class C fly ash indicated minimal leaching from most fly ashes tested. However, the addition of NOx removal equipment might result in higher levels of ammonia in the fly ash. We have recently been testing fly ash with a wide range of pH (3.7-12.4) originating from systems with NOx removal equipment. Leaching experiments were done using dilute CaCl2 solutions in batch and columns and a batch nitric acid method. All methods indicated that the leaching of heavy metals was different in the highest ammonia sample tested and the high pH sample. However, toxicity testing with the Microtox system has indicated little potential toxicity in leachates except for the fly ash at the highest pH (12.4). When the leachate from the high pH fly ash was neutralized, toxicity was eliminated.

  10. Comparing metal leaching and toxicity from high pH, low pH, and high ammonia fly ash

    SciTech Connect (OSTI)

    Palumbo, Anthony V.; Tarver, Jana R.; Fagan, Lisa A.; McNeilly, Meghan S.; Ruther, Rose; Fisher, L. S.; Amonette, James E.

    2007-07-01

    Previous work with both class F and class C fly ash indicated minimal leaching from most fly ashes tested. However, the addition of NOx removal equipment might result in higher levels of ammonia in the fly ash. We have recently been testing fly ash with a wide range of pH (3.712.4) originating from systems with NOx removal equipment. Leaching experiments were done using dilute CaCl2 solutions in batch and columns and a batch nitric acid method. All methods indicated that the leaching of heavy metals was different in the highest ammonia sample tested and the high pH sample. However, toxicity testing with the Microtox* system has indicated little potential toxicity in leachates except for the fly ash at the highest pH (12.4). When the leachate from the high pH fly ash was neutralized, toxicity was eliminated.

  11. Toxicity mitigation and solidification of municipal solid waste incinerator fly ash using alkaline activated coal ash

    SciTech Connect (OSTI)

    Ivan Diaz-Loya, E.; Allouche, Erez N.; Eklund, Sven; Joshi, Anupam R.; Kupwade-Patil, Kunal

    2012-08-15

    Highlights: Black-Right-Pointing-Pointer Incinerator fly ash (IFA) is added to an alkali activated coal fly ash (CFA) matrix. Black-Right-Pointing-Pointer Means of stabilizing the incinerator ash for use in construction applications. Black-Right-Pointing-Pointer Concrete made from IFA, CFA and IFA-CFA mixes was chemically characterized. Black-Right-Pointing-Pointer Environmentally friendly solution to IFA disposal by reducing its toxicity levels. - Abstract: Municipal solid waste (MSW) incineration is a common and effective practice to reduce the volume of solid waste in urban areas. However, the byproduct of this process is a fly ash (IFA), which contains large quantities of toxic contaminants. The purpose of this research study was to analyze the chemical, physical and mechanical behaviors resulting from the gradual introduction of IFA to an alkaline activated coal fly ash (CFA) matrix, as a mean of stabilizing the incinerator ash for use in industrial construction applications, where human exposure potential is limited. IFA and CFA were analyzed via X-ray fluorescence (XRF), X-ray diffraction (XRD) and Inductive coupled plasma (ICP) to obtain a full chemical analysis of the samples, its crystallographic characteristics and a detailed count of the eight heavy metals contemplated in US Title 40 of the Code of Federal Regulations (40 CFR). The particle size distribution of IFA and CFA was also recorded. EPA's Toxicity Characteristic Leaching Procedure (TCLP) was followed to monitor the leachability of the contaminants before and after the activation. Also images obtained via Scanning Electron Microscopy (SEM), before and after the activation, are presented. Concrete made from IFA, CFA and IFA-CFA mixes was subjected to a full mechanical characterization; tests include compressive strength, flexural strength, elastic modulus, Poisson's ratio and setting time. The leachable heavy metal contents (except for Se) were below the maximum allowable limits and in many cases even below the reporting limit. The leachable Chromium was reduced from 0.153 down to 0.0045 mg/L, Arsenic from 0.256 down to 0.132 mg/L, Selenium from 1.05 down to 0.29 mg/L, Silver from 0.011 down to .001 mg/L, Barium from 2.06 down to 0.314 mg/L and Mercury from 0.007 down to 0.001 mg/L. Although the leachable Cd exhibited an increase from 0.49 up to 0.805 mg/L and Pd from 0.002 up to 0.029 mg/L, these were well below the maximum limits of 1.00 and 5.00 mg/L, respectively.

  12. Advanced regenerative thermal oxidation (RTO) technology for air toxics control - selected case histories

    SciTech Connect (OSTI)

    Seiwert, J.J. Jr.

    1997-12-31

    Advanced design regenerative thermal oxidation (RTO) systems have been developed and are in commercial scale use for control of process emissions containing air toxics (HAPs) and VOCs. High operating temperatures coupled with high thermal energy recovery efficiencies inherent with RTO technology provide for high destruction efficiencies while minimizing formation of objectionable combustion byproducts. These results are achieved with low system operating costs. This paper covers development of advanced design commercial RTO systems for control of air emissions from several important commercial processes: total reduced sulfur (TRS) and other HAPs/VOC emissions from pulp mill processes. Chlorinated organics and other HAPs/VOC emissions from pharmaceutical manufacturing operations. The data presented represent the first commercial scale application of RTO technology to abate emissions from these processes. Particular design features required for each specific process, in order to provide reliable, safe and effective systems, are reviewed. Emissions abatement performance, as well as operational data, are presented for the systems.

  13. Mass-transport models to predict toxicity of inhaled gases in the upper respiratory tract

    SciTech Connect (OSTI)

    Hubal, E.A.C.; Fedkiw, P.S.; Kimbell, J.S. [North Carolina State Univ., Raleigh, NC (United States)

    1996-04-01

    Mass-transport (the movement of a chemical species) plays an important role in determining toxic responses of the upper respiratory tract (URT) to inhaled chemicals. Mathematical dosimetry models incorporate physical characteristics of mass transport and are used to predict quantitative uptake (absorption rate) and distribution of inhaled gases and vapors in the respiratory tract. Because knowledge of dose is an essential component of quantitative risk assessment, dosimetry modeling plays an important role in extrapolation of animal study results to humans. A survey of existing mathematical dosimetry models for the URT is presented, limitations of current models are discussed, and adaptations of existing models to produce a generally applicable model are suggested. Reviewed URT dosimetry models are categorized as early, lumped-parameter, and distributed-parameter models. Specific examples of other relevant modeling work are also presented. 35 refs., 11 figs., 1 tab.

  14. Proton Beam Craniospinal Irradiation Reduces Acute Toxicity for Adults With Medulloblastoma

    SciTech Connect (OSTI)

    Brown, Aaron P.; Barney, Christian L.; Grosshans, David R.; McAleer, Mary Frances; Groot, John F. de; Puduvalli, Vinay K.; Tucker, Susan L.; Crawford, Cody N.; Khan, Meena; Khatua, Soumen; Gilbert, Mark R.; Brown, Paul D.; Mahajan, Anita

    2013-06-01

    Purpose: Efficacy and acute toxicity of proton craniospinal irradiation (p-CSI) were compared with conventional photon CSI (x-CSI) for adults with medulloblastoma. Methods and Materials: Forty adult medulloblastoma patients treated with x-CSI (n=21) or p-CSI (n=19) at the University of Texas MD Anderson Cancer Center from 2003 to 2011 were retrospectively reviewed. Median CSI and total doses were 30.6 and 54 Gy, respectively. The median follow-up was 57 months (range 4-103) for x-CSI patients and 26 months (range 11-63) for p-CSI. Results: p-CSI patients lost less weight than x-CSI patients (1.2% vs 5.8%; P=.004), and less p-CSI patients had >5% weight loss compared with x-CSI (16% vs 64%; P=.004). p-CSI patients experienced less grade 2 nausea and vomiting compared with x-CSI (26% vs 71%; P=.004). Patients treated with x-CSI were more likely to have medical management of esophagitis than p-CSI patients (57% vs 5%, P<.001). p-CSI patients had a smaller reduction in peripheral white blood cells, hemoglobin, and platelets compared with x-CSI (white blood cells 46% vs 55%, P=.04; hemoglobin 88% vs 97%, P=.009; platelets 48% vs 65%, P=.05). Mean vertebral doses were significantly associated with reductions in blood counts. Conclusions: This report is the first analysis of clinical outcomes for adult medulloblastoma patients treated with p-CSI. Patients treated with p-CSI experienced less treatment-related morbidity including fewer acute gastrointestinal and hematologic toxicities.

  15. Intervention of D-glucose ameliorates the toxicity of streptozotocin in accessory sex organs of rat

    SciTech Connect (OSTI)

    Vikram, A.; Tripathi, D.N.; Ramarao, P. [Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab-160062 (India); Jena, G.B. [Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab-160062 (India)], E-mail: gbjena@gmail.com

    2008-01-01

    Streptozotocin (STZ) is a naturally occurring compound isolated from Streptomyces achromogens. It is used extensively for inducing diabetes in experimental animals. Diabetes mellitus is known to have proven adverse effects on male sexual organs and their reproductive functions. The atrophy of prostate gland and other organs of the genitourinary tract were observed in experimental diabetic animals. STZ exhibits a structural resemblance to D-glucose due to the presence of sugar moiety in its structure. Pancreatic {beta}-cells mainly contain GLUT1 and GLUT2 glucose transporters. Possibly due to structural resemblance, STZ and D-glucose, share a common recognition site for entry into the {beta}-cells. The objective of the present study is to evaluate the effect of D-glucose on STZ-induced toxicity in accessory sex organs of male rats. Animals were kept on overnight fasting. One group received vehicle and served as negative control, while all other groups were given STZ (45 mg/kg). Animals that received only STZ served as positive control. The effect of D-glucose was studied on STZ treated animals with different dosage of D-glucose (250, 500, 1000 and 2000 mg/kg). Restoration of body weight, plasma glucose and plasma insulin was evident only at 1000 and 2000 mg/kg of D-glucose. The protective effect of D-glucose is evident only when it is administered simultaneously with STZ. In the present investigation, we report that simultaneous administration of D-glucose along with STZ ameliorates STZ-induced toxicity. This is evident from the restoration of accessory sex organ's weight, cellular morphology as well as insulin level.

  16. In situ retreival of contaminants or other substances using a barrier system and leaching solutions and components, processes and methods relating thereto

    DOE Patents [OSTI]

    Nickelson, Reva A.; Walsh, Stephanie; Richardson, John G.; Dick, John R.; Sloan, Paul A.

    2005-06-28

    Processes and methods relating to treating contaminants and collecting desired substances from a zone of interest using subterranean collection and containment barriers. Tubular casings having interlock structures are used to create subterranean barriers for containing and treating buried waste and its effluents. The subterranean barrier includes an effluent collection system. Treatment solutions provided to the zone of interest pass therethrough and are collected by the barrier and treated or recovered, allowing on-site remediation. Barrier components may be used to in the treatment by collecting or removing contaminants or other materials from the zone of interest.

  17. SU-E-J-149: Establishing the Relationship Between Pre-Treatment Lung Ventilation, Dose, and Toxicity Outcome

    SciTech Connect (OSTI)

    Mistry, N; D'Souza, W; Sornsen de Koste, J; Senan, S

    2014-06-01

    Purpose: Recently, there has been an interest in incorporating functional information in treatment planning especially in thoracic tumors. The rationale is that healthy lung regions need to be spared from radiation if possible to help achieve better control on toxicity. However, it is still unclear whether high functioning regions need to be spared or have more capacity to deal with the excessive radiation as compared to the compromised regions of the lung. Our goal with this work is to establish the tools by which we can establish a relationship between pre-treatment lung function, dose, and radiographic outcomes of lung toxicity. Methods: Treatment planning was performed using a single phase of a 4DCT scan, and follow-up anatomical CT scans were performed every 3 months for most patients. In this study, we developed the pipeline of tools needed to analyze such a large dataset, while trying to establish a relationship between function, dose, and outcome. Pre-treatment lung function was evaluated using a recently published technique that evaluates Fractional Regional Ventilation (FRV). All images including the FRV map and the individual follow-up anatomical CT images were all spatially matched to the planning CT using a diffusion based Demons image registration algorithm. Change in HU value was used as a metric to capture the effects of lung toxicity. To validate the findings, a radiologist evaluated the follow-up anatomical CT images and scored lung toxicity. Results: Initial experience in 1 patient shows a relationship between the pre-treatment lung function, dose and toxicity outcome. The results are also correlated to the findings by the radiologist who was blinded to the analysis or dose. Conclusion: The pipeline we have established to study this enables future studies in large retrospective studies. However, the tools are dependent on the fidelity of 4DCT reconstruction for accurate evaluation of regional ventilation. Patent Pending for the technique presented in this work to evaluate FRV incorporating mass correction.

  18. Building Toxic Metal Characterization and Decontamination Report: Area 6, Building 914

    SciTech Connect (OSTI)

    NSTec Industrial Hygiene

    2011-08-15

    The purpose of this report is to outline the toxic metal characterization and decontamination efforts in Area 6, Building 914. This includes the initial building inspection, the hotspot sampling, results/findings, building cleanup, and the verification sampling. Building 914 is a steel light frame building that was constructed in 1992. It is about 16,454 square feet, and five employees are assigned to this building. According to the building's floor plan blueprints, it could be inferred that this building was once a Wiremen/Lineman shop. In 2002-2004, the National Nuclear Security Administration Nevada Site Office embarked on a broad characterization of beryllium (Be) surface concentrations throughout the North Las Vegas Facility, the Nevada National Security Site (NNSS), and ancillary facilities like the Special Technologies Laboratory, Remote Sensing Laboratory, etc. Building 914 was part of this characterization. The results of the 2002 study illustrated that the metal housekeeping limits were within acceptable limits and from a Be standpoint, the building was determined to be fit for occupancy. On March 2, 2011, based on a request from Building 914 users, National Security Technologies, LLC (NSTec) Industrial Hygiene (IH) collected bulk samples from the southwest corner of Building 914 at heights above 6 feet where black dust had been noticed on this particular wall. IH conducted surface swipe sampling of the area and analyzed the samples for toxic metals, namely, beryllium (Be), cadmium (Cd), chromium (Cr), lead (Pb), and manganese (Mn). The sample results indicated values two to four times above the housekeeping threshold for Be, Cd, Cr, Pb, and Mn. Subsequently, the facility was closed and posted; the necessary personnel were notified; and controls were instituted for ingress and egress of the building. On March 17, 2011, IH performed an extensive sampling event involving the entire warehouse in accordance with NSTec Organization Procedure OP-P250.004, Sampling Procedures. Analysis of the results from this exercise illustrated that toxic metal contamination was ubiquitous throughout the warehouse section of this building but did not extend into the office, restroom, and break room areas. On March 22, 2011, a planning meeting was held with Environment, Safety, Health & Quality management; Operations & Infrastructure (O&I) mangement; Facility Management; Occupational Medicine; O&I Operations; and IH. After a brief discussion concerning the salient facts of the surface sample results, it was agreed that the facility and its contents required cleaning. The facility would then be re-sampled to verify cleanliness and suitability for re-occupancy. On April 18, 2011, warehouse cleanup activites began. On July 5, 2011, upon receipt of the results from the last cleaned section, the cleanup operations were concluded. The building was statistically determined to be clean; thus, it could be reoccupied and the warehouse operations could resume immediately.

  19. Multi-parameter in vitro toxicity testing of crizotinib, sunitinib, erlotinib, and nilotinib in human cardiomyocytes

    SciTech Connect (OSTI)

    Doherty, Kimberly R.; Wappel, Robert L.; Talbert, Dominique R.; Trusk, Patricia B.; Moran, Diarmuid M.; Kramer, James W.; Brown, Arthur M.; Shell, Scott A.; Bacus, Sarah

    2013-10-01

    Tyrosine kinase inhibitors (TKi) have greatly improved the treatment and prognosis of multiple cancer types. However, unexpected cardiotoxicity has arisen in a subset of patients treated with these agents that was not wholly predicted by pre-clinical testing, which centers around animal toxicity studies and inhibition of the human Ether--go-go-Related Gene (hERG) channel. Therefore, we sought to determine whether a multi-parameter test panel assessing the effect of drug treatment on cellular, molecular, and electrophysiological endpoints could accurately predict cardiotoxicity. We examined how 4 FDA-approved TKi agents impacted cell viability, apoptosis, reactive oxygen species (ROS) generation, metabolic status, impedance, and ion channel function in human cardiomyocytes. The 3 drugs clinically associated with severe cardiac adverse events (crizotinib, sunitinib, nilotinib) all proved to be cardiotoxic in our in vitro tests while the relatively cardiac-safe drug erlotinib showed only minor changes in cardiac cell health. Crizotinib, an ALK/MET inhibitor, led to increased ROS production, caspase activation, cholesterol accumulation, disruption in cardiac cell beat rate, and blockage of ion channels. The multi-targeted TKi sunitinib showed decreased cardiomyocyte viability, AMPK inhibition, increased lipid accumulation, disrupted beat pattern, and hERG block. Nilotinib, a second generation Bcr-Abl inhibitor, led to increased ROS generation, caspase activation, hERG block, and an arrhythmic beat pattern. Thus, each drug showed a unique toxicity profile that may reflect the multiple mechanisms leading to cardiotoxicity. This study demonstrates that a multi-parameter approach can provide a robust characterization of drug-induced cardiomyocyte damage that can be leveraged to improve drug safety during early phase development. - Highlights: TKi with known adverse effects show unique cardiotoxicity profiles in this panel. Crizotinib increases ROS, apoptosis, and cholesterol as well as alters beat rate. Sunitinib inhibits AMPK, increases lipids and alters the cardiac beat pattern. Nilotinib causes ROS and caspase activation, decreased lipids and arrhythmia. Erlotinib did not impact ROS, caspase, or lipid levels or affect the beat pattern.

  20. Unexpected gender difference in sensitivity to the acute toxicity of dioxin in mice

    SciTech Connect (OSTI)

    Pohjanvirta, Raimo; Miettinen, Hanna; Sankari, Satu; Hegde, Nagabhooshan; Lindn, Jere; Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, FI-00014 University of Helsinki

    2012-07-15

    The acute toxicity of the ubiquitous environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) varies widely among species and strains. Previous studies in rats have established that females are approximately 2-fold more sensitive to TCDD lethality than males. However, there is a surprising gap in the literature regarding possible gender-related sensitivity differences in mice. In the present study, by using three substrains of TCDD-sensitive C57BL/6 mice and transgenic mice on this background, we demonstrated that: 1) in contrast to the situation in rats, female mice are the more resistant gender; 2) the magnitude of the divergence between male and female mice depends on the substrain, but can amount to over 10-fold; 3) AH receptor protein expression levels or mutations in the primary structure of this receptor are not involved in the resistance of female mice of a C57BL/6 substrain, despite their acute LD{sub 50} for TCDD being over 5000 ?g/kg; 4) transgenic mice that globally express the rat wildtype AH receptor follow the mouse type of gender difference; 5) in gonadectomized mice, ovarian estrogens appear to enhance TCDD resistance, whereas testicular androgens seem to augment TCDD susceptibility; and 6) the gender difference correlates best with the severity of liver damage, which is also reflected in hepatic histopathology and the expression of pro-inflammatory cytokines, especially IL-6. Hence, the two closely related rodent species most often employed in toxicological risk characterization studies, rat and mouse, represent opposite examples of the influence of gender on dioxin sensitivity, further complicating the risk assessment of halogenated aromatic hydrocarbons. -- Highlights: ? In contrast to rats, male mice are more sensitive to TCDD toxicity than female mice. ? The resistance of female C57BL/6Kuo mice matches or exceeds that of male DBA/2 mice. ? The resistance of female C57BL/6Kuo mice is not based on AHR structure or abundance. ? Both androgens and estrogens appear to influence TCDD sensitivity. ? TCDD sensitivity correlates best with the severity of lesions in the liver.

  1. Hematologic Toxicity in RTOG 0418: A Phase 2 Study of Postoperative IMRT for Gynecologic Cancer

    SciTech Connect (OSTI)

    Klopp, Ann H.; Moughan, Jennifer; Portelance, Lorraine; Miller, Brigitte E.; Salehpour, Mohammad R.; Hildebrandt, Evangeline; Nuanjing, Jenny; D'Souza, David; Souhami, Luis; Small, William; Gaur, Rakesh; Jhingran, Anuja

    2013-05-01

    Purpose: Intensity modulated radiation therapy (IMRT), compared with conventional 4-field treatment, can reduce the volume of bone marrow irradiated. Pelvic bone marrow sparing has produced a clinically significant reduction in hematologic toxicity (HT). This analysis investigated HT in Radiation Therapy Oncology Group (RTOG) 0418, a prospective study to test the feasibility of delivering postoperative IMRT for cervical and endometrial cancer in a multiinstitutional setting. Methods and Materials: Patients in the RTOG 0418 study were treated with postoperative IMRT to 50.4 Gy to the pelvic lymphatics and vagina. Endometrial cancer patients received IMRT alone, whereas patients with cervical cancer received IMRT and weekly cisplatin (40 mg/m{sup 2}). Pelvic bone marrow was defined within the treatment field by using a computed tomography density-based autocontouring algorithm. The volume of bone marrow receiving 10, 20, 30, and 40 Gy and the median dose to bone marrow were correlated with HT, graded by Common Terminology Criteria for Adverse Events, version 3.0, criteria. Results: Eighty-three patients were eligible for analysis (43 with endometrial cancer and 40 with cervical cancer). Patients with cervical cancer treated with weekly cisplatin and pelvic IMRT had grades 1-5 HT (23%, 33%, 25%, 0%, and 0% of patients, respectively). Among patients with cervical cancer, 83% received 5 or more cycles of cisplatin, and 90% received at least 4 cycles of cisplatin. The median percentage volume of bone marrow receiving 10, 20, 30, and 40 Gy in all 83 patients, respectively, was 96%, 84%, 61%, and 37%. Among cervical cancer patients with a V40 >37%, 75% had grade 2 or higher HT compared with 40% of patients with a V40 less than or equal to 37% (P =.025). Cervical cancer patients with a median bone marrow dose of >34.2 Gy also had higher rates of grade ?2 HT than did those with a dose of ?34.2 Gy (74% vs 43%, P=.049). Conclusions: Pelvic IMRT with weekly cisplatin is associated with low rates of HT and high rates of weekly cisplatin use. The volume of bone marrow receiving 40 Gy and the median dose to bone marrow correlated with higher rates of grade ?2 toxicity among patients receiving weekly cisplatin (cervical cancer patients). Evaluation and limitation of the volume of bone marrow treated with pelvic IMRT is warranted in patients receiving concurrent chemotherapy.

  2. Fluoride-elicited developmental testicular toxicity in rats: Roles of endoplasmic reticulum stress and inflammatory response

    SciTech Connect (OSTI)

    Zhang, Shun; Jiang, Chunyang; Liu, Hongliang; Guan, Zhizhong; Zeng, Qiang; Zhang, Cheng; Lei, Rongrong; Xia, Tao; Gao, Hui; Yang, Lu; Chen, Yihu; Wu, Xue; Zhang, Xiaofei; Cui, Yushan; Yu, Linyu; Wang, Zhenglun; Wang, Aiguo

    2013-09-01

    Long-term excessive fluoride intake is known to be toxic and can damage a variety of organs and tissues in the human body. However, the molecular mechanisms underlying fluoride-induced male reproductive toxicity are not well understood. In this study, we used a rat model to simulate the situations of human exposure and aimed to evaluate the roles of endoplasmic reticulum (ER) stress and inflammatory response in fluoride-induced testicular injury. SpragueDawley rats were administered with sodium fluoride (NaF) at 25, 50 and 100 mg/L via drinking water from pre-pregnancy to gestation, birth and finally to post-puberty. And then the testes of male offspring were studied at 8 weeks of age. Our results demonstrated that fluoride treatment increased MDA accumulation, decreased SOD activity, and enhanced germ cell apoptosis. In addition, fluoride elevated mRNA and protein levels of glucose-regulated protein 78 (GRP78), inositol requiring ER-to-nucleus signal kinase 1 (IRE1), and C/EBP homologous protein (CHOP), indicating activation of ER stress signaling. Furthermore, fluoride also induced testicular inflammation, as manifested by gene up-regulation of tumor necrosis factor-? (TNF-?), interleukin-1? (IL-1?), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in a nuclear factor-?B (NF-?B)-dependent manner. These were associated with marked histopathological lesions including injury of spermatogonia, decrease of spermatocytes and absence of elongated spermatids, as well as severe ultrastructural abnormalities in testes. Taken together, our results provide compelling evidence that ER stress and inflammation would be novel and significant mechanisms responsible for fluoride-induced disturbance of spermatogenesis and germ cell loss in addition to oxidative stress. - Highlights: We used a rat model to simulate the situations of human fluoride (F) exposure. Developmental F exposure induces testicular damage related with oxidative stress. Endoplasmic reticulum stress is involved in testis disorder and germ cell apoptosis. Inflammatory response is implicated in impaired spermatogenesis and germ cell loss.

  3. Biofiltration control of VOC and air toxic emissions: n-Butane and benzene

    SciTech Connect (OSTI)

    Allen, E.R.

    1996-12-31

    n-Butane and benzene vapors are routinely observed in urban atmospheres. Their presence in urban airsheds is of concern because of their ozone production potential as volatile organic compounds (VOCs) and/or potential toxicity. Also, these saturated hydrocarbons are representative of airborne aliphatic and aromatic compounds. Separate laboratory studies have been conducted on the biological elimination of n-butane (n-C{sub 4}H{sub 10}) and benzene (C{sub 6}H{sub 6}) from airstreams using treated compost biofilters. The removal efficiencies were found to exceed 90% for a conditioned biofilter medium and pollutant low concentrations (< 25 ppm) and zeroth order kinetics at higher concentrations (> 100 ppm), whereas benzene vapor elimination followed zeroth order kinetics at concentrations up to 200 ppm. The maximum n-butane and benzene elimination capacities observed for the compost biofilters and conditions employed were 25 and 70 g pollutant m{sup -3} h{sup -1}, respectively. 13 refs., 6 figs., 2 tabs.

  4. A novel nanoparticle-based disposable electrochemical immunosensor for diagnosis of exposure to toxic organophosphorus agents

    SciTech Connect (OSTI)

    Lu, Donglai; Wang, Jun; Wang, Limin; Du, Dan; Timchalk, Charles; Barry, Richard C.; Lin, Yuehe

    2011-11-15

    We present a novel disposable electrochemical immunosensor for highly selective and sensitive detection of organophosphorylated butyrylcholinesterase (OP-BChE), a specific biomarker for exposure to toxic organophosphorus agents. In our new approach, the zirconia nanoparticles (ZrO-2) were employed to selectively capture the OP moiety of OP-BChE adducts, and followed by quantum dot (QD)-tagged anti-BChE antibodies for amplified quantification. The captured CdSe-QD tags can be sensitively detected by stripping voltammetry using in situ bismuth-plating method. The OP agent, diisopropylfluorophosphate (DFP), was selected to prepare OP-BChE adducts in various matrices. The formation of OP-BChE adducts in plasma sample was confirmed using mass spectroscopy. The developed electrochemical immunosensor demonstrates a highly linear voltammetric response over the range of 0.1 to 30 nM OP-BChE. Moreover, the immunosensor has been successfully applied for the detection of OP-BChE adducts in the plasma samples. This novel nanoparticle-based electrochemical immunosensor thus provides an alternative way for designing simple, fast, sensitive, and cost-effective sensing platform for on-site screening/evaluating exposure to a variety of OP agents.

  5. [Toxicity studies of mild gasification products]. [Quarterly report, October 1, 1992--December 31, 1992

    SciTech Connect (OSTI)

    Not Available

    1992-12-31

    Mild gasification of coal is a technology being developed by the United States Department of Energy and private industry with the hope that a cleaner method of coal use can help meet future energy needs. As the technology develops and its commercial use becomes a more viable possibility, efforts are being made to study the safety or possible toxicity of the mild gasification products. DOE and the National Institute for occupational Safety and Health (NIOSH) are cooperating through an interagency agreement to examine some of these products for their genotoxic potential. NIOSH has studied the mutagenicity of several mild gasification product samples using the Ames Salmonella/microsomal assay. As reported earlier PSIS{number_sign}830331 failed to demonstrate genotoxic activity in the Ames assay under all conditions tested. Since the mild gasification products are complex mixtures, interactions between various components are likely to occur. Such interactions between various components of complex mixtures may increase or decrease genotoxic activity in short-term assays like the Ames test. Although all synergistic interactions may not be detailed, the separate analysis of those components in several classes provides a more accurate view of the genotoxicity of each component and better allows for chemical characterization of the possible mutagens in the mixture. NIOSH has performed mutagenicity studies on the subfractions of PSIS{number_sign}830331. The results of those studies are detailed in this report.

  6. Application of powder metallurgy techniques for the development of non-toxic ammunition. Final CRADA report

    SciTech Connect (OSTI)

    Lowden, R.; Kelly, R.

    1997-05-30

    The purpose of the Cooperative Research and Development Agreement (CRADA) between Martin Marietta Energy Systems, Inc., and Delta Frangible Ammunition (DFA), was to identify and evaluate composite materials for the development of small arms ammunition. Currently available small arms ammunition utilizes lead as the major component of the projectile. The introduction of lead into the environment by these projectiles when they are expended is a rapidly increasing environmental problem. At certain levels, lead is a toxic metal to the environment and a continual health and safety concern for firearm users as well as those who must conduct lead recovery operations from the environment. DFA is a leading supplier of high-density mixtures, which will be used to replace lead-based ammunition in specific applications. Current non-lead ammunition has several limitations that prevent it from replacing lead-based ammunition in many applications (such as applications that require ballistics, weapon recoil, and weapon function identical to that of lead-based ammunition). The purpose of the CRADA was to perform the research and development to identify cost-effective materials to be used in small arms ammunition that eventually will be used in commercially viable, environmentally conscious, non-lead, frangible and/or non-frangible, ammunition.

  7. Balboa Pacific Corporation | Open Energy Information

    Open Energy Info (EERE)

    continuous feed waste treatment technology that causes the destructive distillation of toxic or non-toxic organic material, either solid or liquid substances, reducing the...

  8. Mini-lidar sensor for the remote stand-off sensing of chemical/biological substances and method for sensing same

    DOE Patents [OSTI]

    Ray, Mark D.; Sedlacek, Arthur J.

    2003-08-19

    A method and apparatus for remote, stand-off, and high efficiency spectroscopic detection of biological and chemical substances. The apparatus including an optical beam transmitter which transmits a beam having an axis of transmission to a target, the beam comprising at least a laser emission. An optical detector having an optical detection path to the target is provided for gathering optical information. The optical detection path has an axis of optical detection. A beam alignment device fixes the transmitter proximal to the detector and directs the beam to the target along the optical detection path such that the axis of transmission is within the optical detection path. Optical information gathered by the optical detector is analyzed by an analyzer which is operatively connected to the detector.

  9. Reducing the Risks. In the aftermath of a terrorist attack, wastewater utilities may have to contend with decontamination water containing chemical, biological, or radiological substances

    SciTech Connect (OSTI)

    Warren, Linda P.; Hornback, Chris; Strom, Daniel J.

    2006-08-01

    In the aftermath of a chemical, biological, or radiological (CBR) attack, decontamination of people and infrastructure will be needed. Decontamination inevitably produces wastewater, and wastewater treatment plants (WTPs) need to know how to handle decontamination wastewater. This article describes CBR substances; planning, coordinating, and communicating responses across agencies; planning within a utility; coordination with local emergency managers and first responders; mitigating effects of decontamination wastewater; and mitigating effects on utility personnel. Planning for Decontamination Wastewater: A Guide for Utilities, the document on which this article is based, was developed under a cooperative agreement from the U.S. Environmental Protection Agency by the National Association of Clean Water Agencies (NACWA) and its contractor, CH2MHILL, Inc.

  10. Transcriptomics-based identification of developmental toxicants through their interference with cardiomyocyte differentiation of embryonic stem cells

    SciTech Connect (OSTI)

    Dartel, Dorien A.M. van; Pennings, Jeroen L.A.; Schooten, Frederik J. van; Piersma, Aldert H.

    2010-03-15

    The embryonic stem cell test (EST) predicts developmental toxicity based on the inhibition of cardiomyocyte differentiation of embryonic stem cells (ESC). The subjective endpoint, the long culture duration together with the undefined applicability domain and related predictivity need further improvement to facilitate implementation of the EST into regulatory strategies. These aspects may be improved by studying gene expression changes in the ESC differentiation cultures and their modulation by compound exposure using transcriptomics. Here, we tested the developmental toxicants monobutyl phthalate and 6-aminonicotinamide. ESC were allowed to differentiated, and cardiomyocyte differentiation was assessed after 10 days of culture. RNA of solvent controls was collected after 0, 24, 48, 72 and 96 h of exposure, and RNA of developmental-toxicant-exposed cultures was collected after 24 and 96 h. Samples were hybridized to DNA microarrays, and 1355 genes were found differentially expressed among the unexposed experimental groups. These regulated genes were involved in differentiation-related processes, and Principal Component Analysis (PCA) based on these genes showed that the unexposed experimental groups appeared in chronological order in the PCA, which can therefore be regarded as a continuous representation of the differentiation track. The developmental-toxicant-exposed cultures appeared to deviate significantly from this differentiation track, which confirms the compound-modulating effects on the differentiation process. The incorporation of transcriptomics in the EST is expected to provide a more informative and improved endpoint in the EST as compared with morphology, allowing early detection of differentiation modulation. Furthermore, this approach may improve the definition of the applicability domain and predictivity of the EST.

  11. EVALUATION OF BEST AVAILABLE CONTROL TECHNOLOGY FOR TOXICS (TBACT) DOUBLE SHELL TANK FARMS PRIMARY VENTILATION SYSTEM SUPPORTING WASTE TRANSFER OPERATIONS

    SciTech Connect (OSTI)

    KELLY SE; HAASS CC; KOVACH JL; TURNER DA

    2010-06-03

    This report is an evaluation of Best Available Control Technology for Toxics (tBACT) for installation and operation of the Hanford double shell (DST) tank primary ventilation systems. The DST primary ventilation systems are being modified to support Hanford's waste retrieval, mixing, and delivery of single shell tank (SST) and DST waste throught the DST storage system to the Waste Treatment and Immobilization Plant (WTP).

  12. EVALUATION OF BEST AVAILABLE CONTROL TECHNOLOGY FOR TOXICS -TBACT- DOUBLE SHELL TANK FARMS PRIMARY VENTILATION SYSTEMS SUPPORTING WASTE TRANSFER OPERATIONS

    SciTech Connect (OSTI)

    HAAS CC; KOVACH JL; KELLY SE; TURNER DA

    2010-06-24

    This report is an evaluation of Best Available Control Technology for Toxics (tBACT) for installation and operation of the Hanford double shell (DST) tank primary ventilation systems. The DST primary ventilation systems are being modified to support Hanford's waste retrieval, mixing, and delivery of single shell tank (SST) and DST waste through the DST storage system to the Waste Treatment and Immobilizaiton Plant (WTP).

  13. SU-E-T-206: Improving Radiotherapy Toxicity Based On Artificial Neural Network (ANN) for Head and Neck Cancer Patients

    SciTech Connect (OSTI)

    Cho, Daniel D; Wernicke, A Gabriella; Nori, Dattatreyudu; Chao, KSC; Parashar, Bhupesh; Chang, Jenghwa [Weill Cornell Medical College, NY, NY (United States)

    2014-06-01

    Purpose/Objective(s): The aim of this study is to build the estimator of toxicity using artificial neural network (ANN) for head and neck cancer patients Materials/Methods: An ANN can combine variables into a predictive model during training and considered all possible correlations of variables. We constructed an ANN based on the data from 73 patients with advanced H and N cancer treated with external beam radiotherapy and/or chemotherapy at our institution. For the toxicity estimator we defined input data including age, sex, site, stage, pathology, status of chemo, technique of external beam radiation therapy (EBRT), length of treatment, dose of EBRT, status of post operation, length of follow-up, the status of local recurrences and distant metastasis. These data were digitized based on the significance and fed to the ANN as input nodes. We used 20 hidden nodes (for the 13 input nodes) to take care of the correlations of input nodes. For training ANN, we divided data into three subsets such as training set, validation set and test set. Finally, we built the estimator for the toxicity from ANN output. Results: We used 13 input variables including the status of local recurrences and distant metastasis and 20 hidden nodes for correlations. 59 patients for training set, 7 patients for validation set and 7 patients for test set and fed the inputs to Matlab neural network fitting tool. We trained the data within 15% of errors of outcome. In the end we have the toxicity estimation with 74% of accuracy. Conclusion: We proved in principle that ANN can be a very useful tool for predicting the RT outcomes for high risk H and N patients. Currently we are improving the results using cross validation.

  14. Dosimetric and Late Radiation Toxicity Comparison Between Iodine-125 Brachytherapy and Stereotactic Radiation Therapy for Juxtapapillary Choroidal Melanoma

    SciTech Connect (OSTI)

    Krema, Hatem

    2013-07-01

    Purpose: To compare the dose distributions and late radiation toxicities for {sup 125}I brachytherapy (IBT) and stereotactic radiation therapy (SRT) in the treatment of juxtapapillary choroidal melanoma. Methods: Ninety-four consecutive patients with juxtapapillary melanoma were reviewed: 30 have been treated with IBT and 64 with SRT. Iodine-125 brachytherapy cases were modeled with plaque simulator software for dosimetric analysis. The SRT dosimetric data were obtained from the Radionics XKnife RT3 software. Mean doses at predetermined intraocular points were calculated. Kaplan-Meier estimates determined the actuarial rates of late toxicities, and the logrank test compared the estimates. Results: The median follow-up was 46 months in both cohorts. The 2 cohorts were balanced with respect to pretreatment clinical and tumor characteristics. Comparisons of radiation toxicity rates between the IBT and SRT cohorts yielded actuarial rates at 50 months for cataracts of 62% and 75% (P=.1), for neovascular glaucoma 8% and 47% (P=.002), for radiation retinopathy 59% and 89% (P=.0001), and for radiation papillopathy 39% and 74% (P=.003), respectively. Dosimetric comparisons between the IBT and SRT cohorts yielded mean doses of 12.8 and 14.1 Gy (P=.56) for the lens center, 17.6 and 19.7 Gy (P=.44) for the lens posterior pole, 13.9 and 10.8 Gy (P=.30) for the ciliary body, 61.9 and 69.7 Gy (P=.03) for optic disc center, and 48.9 and 60.1 Gy (P<.0001) for retina at 5-mm distance from tumor margin, respectively. Conclusions: Late radiation-induced toxicities were greater with SRT, which is secondary to the high-dose exposure inherent to the technique as compared with IBT. When technically feasible, IBT is preferred to treat juxtapapillary choroidal melanoma.

  15. VirtualToxLab A platform for estimating the toxic potential of drugs, chemicals and natural products

    SciTech Connect (OSTI)

    Vedani, Angelo; Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056 Basel ; Dobler, Max; Smieko, Martin

    2012-06-01

    The VirtualToxLab is an in silico technology for estimating the toxic potential (endocrine and metabolic disruption, some aspects of carcinogenicity and cardiotoxicity) of drugs, chemicals and natural products. The technology is based on an automated protocol that simulates and quantifies the binding of small molecules towards a series of proteins, known or suspected to trigger adverse effects. The toxic potential, a non-linear function ranging from 0.0 (none) to 1.0 (extreme), is derived from the individual binding affinities of a compound towards currently 16 target proteins: 10 nuclear receptors (androgen, estrogen ?, estrogen ?, glucocorticoid, liver X, mineralocorticoid, peroxisome proliferator-activated receptor ?, progesterone, thyroid ?, and thyroid ?), four members of the cytochrome P450 enzyme family (1A2, 2C9, 2D6, and 3A4), a cytosolic transcription factor (aryl hydrocarbon receptor) and a potassium ion channel (hERG). The interface to the technology allows building and uploading molecular structures, viewing and downloading results and, most importantly, rationalizing any prediction at the atomic level by interactively analyzing the binding mode of a compound with its target protein(s) in real-time 3D. The VirtualToxLab has been used to predict the toxic potential for over 2500 compounds: the results are posted on (http://www.virtualtoxlab.org). The free platform the OpenVirtualToxLab is accessible (in clientserver mode) over the Internet. It is free of charge for universities, governmental agencies, regulatory bodies and non-profit organizations. -- Highlights: ? In silico technology for estimating the toxic potential of drugs and chemicals. ? Simulation of binding towards 16 proteins suspected to trigger adverse effects. ? Mechanistic interpretation and real-time 3D visualization. ? Accessible over the Internet. ? Free of charge for universities, governmental agencies, regulatory bodies and NPOs.

  16. Dosimetric Predictors of Duodenal Toxicity After Intensity Modulated Radiation Therapy for Treatment of the Para-aortic Nodes in Gynecologic Cancer

    SciTech Connect (OSTI)

    Verma, Jonathan [Department of Radiation Oncology, University of Miami Miller School of Medicine, Miami, Florida (United States); Sulman, Erik P.; Jhingran, Anuja [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Tucker, Susan L. [Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Rauch, Gaiane M. [Department of Radiology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Eifel, Patricia J. [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Klopp, Ann H., E-mail: aklopp@mdanderson.org [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States)

    2014-02-01

    Purpose: To determine the incidence of duodenal toxicity in patients receiving intensity modulated radiation therapy (IMRT) for treatment of para-aortic nodes and to identify dosimetric parameters predictive of late duodenal toxicity. Methods and Materials: We identified 105 eligible patients with gynecologic malignancies who were treated with IMRT for gross metastatic disease in the para-aortic nodes from January 1, 2005, through December 31, 2009. Patients were treated to a nodal clinical target volume to 45 to 50.4 Gy with a boost to 60 to 66 Gy. The duodenum was contoured, and dosimetric data were exported for analysis. Duodenal toxicity was scored according to Radiation Therapy Oncology Group criteria. Univariate Cox proportional hazards analysis and recursive partitioning analysis were used to determine associations between dosimetric variables and time to toxicity and to identify the optimal threshold that separated patients according to risk of toxicity. Results: Nine of the 105 patients experienced grade 2 to grade 5 duodenal toxicity, confirmed by endoscopy in all cases. The 3-year actuarial rate of any duodenal toxicity was 11.7%. A larger volume of the duodenum receiving 55 Gy (V55) was associated with higher rates of duodenal toxicity. The 3-year actuarial rates of duodenal toxicity with V55 above and below 15 cm{sup 3} were 48.6% and 7.4%, respectively (P<.01). In Cox univariate analysis of dosimetric variables, V55 was associated with duodenal toxicity (P=.029). In recursive partitioning analysis, V55 less than 13.94% segregated all patients with duodenal toxicity. Conclusions: Dose-escalated IMRT can safely and effectively treat para-aortic nodal disease in gynecologic malignancies, provided that care is taken to limit the dose to the duodenum to reduce the risk of late duodenal toxicity. Limiting V55 to below 15 cm{sup 3} may reduce the risk of duodenal complications. In cases where the treatment cannot be delivered within these constraints, consideration should be given to other treatment approaches such as resection or initial chemotherapy.

  17. Sulfanegen sodium treatment in a rabbit model of sub-lethal cyanide toxicity

    SciTech Connect (OSTI)

    Brenner, Matthew; Kim, Jae G.; Lee, Jangwoen; Mahon, Sari B.; Lemor, Daniel; Ahdout, Rebecca; Boss, Gerry R.; Blackledge, William; Jann, Lauren; Nagasawa, Herbert T.; Patterson, Steven E.

    2010-11-01

    The aim of this study is to investigate the ability of intramuscular and intravenous sulfanegen sodium treatment to reverse cyanide effects in a rabbit model as a potential treatment for mass casualty resulting from cyanide exposure. Cyanide poisoning is a serious chemical threat from accidental or intentional exposures. Current cyanide exposure treatments, including direct binding agents, methemoglobin donors, and sulfur donors, have several limitations. Non-rhodanese mediated sulfur transferase pathways, including 3-mercaptopyruvate sulfurtransferase (3-MPST) catalyze the transfer of sulfur from 3-MP to cyanide, forming pyruvate and less toxic thiocyanate. We developed a water-soluble 3-MP prodrug, 3-mercaptopyruvatedithiane (sulfanegen sodium), with the potential to provide a continuous supply of substrate for CN detoxification. In addition to developing a mass casualty cyanide reversal agent, methods are needed to rapidly and reliably diagnose and monitor cyanide poisoning and reversal. We use non-invasive technology, diffuse optical spectroscopy (DOS) and continuous wave near infrared spectroscopy (CWNIRS) to monitor physiologic changes associated with cyanide exposure and reversal. A total of 35 animals were studied. Sulfanegen sodium was shown to reverse the effects of cyanide exposure on oxyhemoglobin and deoxyhemoglobin rapidly, significantly faster than control animals when administered by intravenous or intramuscular routes. RBC cyanide levels also returned to normal faster following both intramuscular and intravenous sulfanegen sodium treatment than controls. These studies demonstrate the clinical potential for the novel approach of supplying substrate for non-rhodanese mediated sulfur transferase pathways for cyanide detoxification. DOS and CWNIRS demonstrated their usefulness in optimizing the dose of sulfanegen sodium treatment.

  18. Protective effects and mechanisms of curcumin on podophyllotoxin toxicity in vitro and in vivo

    SciTech Connect (OSTI)

    Li, Juan; Dai, Cai-Xia; Sun, Hua; Jin, Lu; State Key Laboratory of New Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203 ; Guo, Chong-Yi; Cao, Wei; Wu, Jie; Tian, Hai-Yan; Luo, Cheng; Ye, Wen-Cai; Jiang, Ren-Wang

    2012-12-01

    Podophyllotoxin (POD) is a naturally occurring lignan with pronounced antineoplastic and antiviral properties. POD binds to tubulin and prevents the formation of mitotic spindle. Although cases of overdose or accidental ingestion are quite often, no specific therapy is currently available to treat the POD intoxication. In the current investigation, the protective effects and mechanisms of curcumin (CUR) on podophyllotoxin toxicity were evaluated in vitro and in vivo. The results showed that CUR could protect POD-induced cytotoxicity by recovering the G2/M arrest and decrease the changes of membrane potential and microtubule structure in Vero cells. A significant decrease of mortality rates was observed in Swiss mice treated by intragastrical administration of POD + CUR as compared with POD alone. The POD + CUR group also exhibited decreases in plasma transaminases, alkaline phosphatase, lactate dehydrogenase, plasma urea, creatinine and malondialdehyde level but elevated superoxide dismutase and glutathione levels as compared to the POD group. Histological examination of the liver and kidney demonstrated less morphological changes in the treatment of POD + CUR as compared with POD alone. The mechanism of the protective effects might be due to the competitive binding of CUR with POD in the same colchicines binding site as revealed by the tubulin polymerization assay and the molecular docking analysis, and the antioxidant activity against the oxidative stress induced by POD. In summary, both in vitro and in vivo data indicated the promising role of CUR as a protective agent against the POD poisoning. Highlights: ? A potential antidote to treat the podophyllotoxin (POD) intoxication is found. ? Curcumin showed promising effects against POD poisoning in vitro and in vivo. ? The mechanisms lie in the antioxidant activity and competitive binding with tubulin.

  19. FINE PARTICAL AND TOXIC METAL EMISSIONS FROM THE COMBUSTION OF SEWAGE SLUDGE/COAL MIXTURES: A SYSTEMATIC ASSESSMENT

    SciTech Connect (OSTI)

    Jost O.L. Wendt; Wayne S. Seames; Art Fernandez

    2003-09-21

    This research project focuses on pollutants from the combustion of mixtures of dried municipal sewage sludge (MSS) and pulverized coal. The objective was to determine potential tradeoffs between CO{sub 2} mitigation through using a CO{sub 2} neutral fuel, such as municipal sewage sludge, and the emergence of other potential problems such as the emission of toxic fly ash particles. The work led to new insight into mechanisms governing the partitioning of major and trace metals from the combustion of sewage sludge, and mixtures of coal and sewage sludge. The research also showed that the co-combustion of coal and sewage sludge emitted fine particulate matter that might potentially cause greater lung injury than that from the combustion of either coal alone or municipal sewage sludge alone. The reason appeared to be that the toxicity measured required the presence of large amounts of both zinc and sulfur in particles that were inhaled. MSS provided the zinc while coal provided the sulfur. Additional research showed that the toxic effects could most likely be engineered out of the process, through the introduction of kaolinite sorbent downstream of the combustion zone, or removing the sulfur from the fuel. These results are consequences of applying ''Health Effects Engineering'' to this issue. Health Effects Engineering is a new discipline arising out of this work, and is derived from using a collaboration of combustion engineers and toxicologists to mitigate the potentially bad health effects from combustion of this biomass fuel.

  20. MicroCantilever (MC) based Robust Sensing Approach for Controlled...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    sensitivity for forensic detection and identification of controlled substance, toxic species, biological molecules, and DNA matching. Current sensor systems require...

  1. Mercury and Air Toxic Element Impacts of Coal Combustion By-Product Disposal and Utilizaton

    SciTech Connect (OSTI)

    David Hassett; Loreal Heebink; Debra Pflughoeft-Hassett; Tera Buckley; Erick Zacher; Mei Xin; Mae Sexauer Gustin; Rob Jung

    2007-03-31

    The University of North Dakota Energy & Environmental Research Center (EERC) conducted a multiyear study to evaluate the impact of mercury and other air toxic elements (ATEs) on the management of coal combustion by-products (CCBs). The ATEs evaluated in this project were arsenic, cadmium, chromium, lead, nickel, and selenium. The study included laboratory tasks to develop measurement techniques for mercury and ATE releases, sample characterization, and release experiments. A field task was also performed to measure mercury releases at a field site. Samples of fly ash and flue gas desulfurization (FGD) materials were collected preferentially from full-scale coal-fired power plants operating both without and with mercury control technologies in place. In some cases, samples from pilot- and bench-scale emission control tests were included in the laboratory studies. Several sets of 'paired' baseline and test fly ash and FGD materials collected during full-scale mercury emission control tests were also included in laboratory evaluations. Samples from mercury emission control tests all contained activated carbon (AC) and some also incorporated a sorbent-enhancing agent (EA). Laboratory release experiments focused on measuring releases of mercury under conditions designed to simulate CCB exposure to water, ambient-temperature air, elevated temperatures, and microbes in both wet and dry conditions. Results of laboratory evaluations indicated that: (1) Mercury and sometimes selenium are collected with AC used for mercury emission control and, therefore, present at higher concentrations than samples collected without mercury emission controls present. (2) Mercury is stable on CCBs collected from systems both without and with mercury emission controls present under most conditions tested, with the exception of vapor-phase releases of mercury exposed to elevated temperatures. (3) The presence of carbon either from added AC or from unburned coal can result in mercury being sorbed onto the CCB when exposed to ambient-temperature air. The environmental performance of the mercury captured on AC used as a sorbent for mercury emission control technologies indicated that current CCB management options will continue to be sufficiently protective of the environment, with the potential exception of exposure to elevated temperatures. The environmental performance of the other ATEs investigated indicated that current management options will be appropriate to the CCBs produced using AC in mercury emission controls.

  2. Removal potential of toxic 2378-substituted PCDD/F from incinerator flue gases by waste-derived activated carbons

    SciTech Connect (OSTI)

    Hajizadeh, Yaghoub; Onwudili, Jude A.; Williams, Paul T.

    2011-06-15

    The application of activated carbons has become a commonly used emission control protocol for the removal or adsorption of persistent organic pollutants from the flue gas streams of waste incinerators. In this study, the 2378-substituted PCDD/F removal efficiency of three types of activated carbons derived from the pyrolysis of refuse derived fuel, textile waste and scrap tyre was investigated and compared with that of a commercial carbon. Experiments were carried out in a laboratory scale fixed-bed reactor under a simulated flue gas at 275 deg. C with a reaction period of four days. The PCDD/F in the solid matrices and exhaust gas, were analyzed using gas chromatography coupled with a triple quadrupole mass spectrometer. In the absence of activated carbon adsorbent, there was a significant increase in the concentration of toxic PCDD/F produced in the reacted flyash, reaching up to 6.6 times higher than in the raw flyash. In addition, there was a substantial release of PCDD/F into the gas phase, which was found in the flue gas trapping system. By application of the different commercial, refuse derived fuel, textile and tyre activated carbons the total PCDD/F toxic equivalent removal efficiencies in the exhaust gas stream were 58%, 57%, 64% and 52%, respectively. In general, the removal of the PCDDs was much higher with an average of 85% compared to PCDFs at 41%. Analysis of the reacted activated carbons showed that there was some formation of PCDD/F, for instance, a total of 60.6 {mu}g I-TEQ kg{sup -1} toxic PCDD/F was formed in the refuse derived fuel activated carbon compared to 34 {mu}g I-TEQ kg{sup -1} in the commercial activated carbon. The activated carbons derived from the pyrolysis of waste, therefore, showed good potential as a control material for PCDD/F emissions in waste incinerator flue gases.

  3. Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Dickinson, Quinn; Bottoms, Scott; Hinchman, Li; McIlwain, Sean; Li, Sheena; Myers, Chad L.; Boone, Charles; Coon, Joshua J.; Hebert, Alexander; Sato, Trey K.; et al

    2016-01-20

    In this study, imidazolium ionic liquids (IILs) underpin promising technologies that generate fermentable sugars from lignocellulose for future biorefineries. However, residual IILs are toxic to fermentative microbes such as Saccharomyces cerevisiae, making IIL-tolerance a key property for strain engineering. To enable rational engineering, we used chemical genomic profiling to understand the effects of IILs on S. cerevisiae. As a result, we found that IILs likely target mitochondria as their chemical genomic profiles closely resembled that of the mitochondrial membrane disrupting agent valinomycin. Further, several deletions of genes encoding mitochondrial proteins exhibited increased sensitivity to IIL. High-throughput chemical proteomics confirmed effectsmore » of IILs on mitochondrial protein levels. IILs induced abnormal mitochondrial morphology, as well as altered polarization of mitochondrial membrane potential similar to valinomycin. Deletion of the putative serine/threonine kinase PTK2 thought to activate the plasma-membrane proton efflux pump Pma1p conferred a significant IIL-fitness advantage. Conversely, overexpression of PMA1 conferred sensitivity to IILs, suggesting that hydrogen ion efflux may be coupled to influx of the toxic imidazolium cation. PTK2 deletion conferred resistance to multiple IILs, including [EMIM]Cl, [BMIM]Cl, and [EMIM]Ac. An engineered, xylose-converting ptk2Δ S. cerevisiae (Y133-IIL) strain consumed glucose and xylose faster and produced more ethanol in the presence of 1 % [BMIM]Cl than the wild-type PTK2 strain. We propose a model of IIL toxicity and resistance. In conclusion, this work demonstrates the utility of chemical genomics-guided biodesign for development of superior microbial biocatalysts for the ever-changing landscape of fermentation inhibitors.« less

  4. A Phase 2 Trial of Once-Weekly Hypofractionated Breast Irradiation: First Report of Acute Toxicity, Feasibility, and Patient Satisfaction

    SciTech Connect (OSTI)

    Dragun, Anthony E.; Quillo, Amy R.; Riley, Elizabeth C.; Roberts, Teresa L.; Hunter, Allison M.; Rai, Shesh N.; Callender, Glenda G.; Jain, Dharamvir; McMasters, Kelly M.; Spanos, William J.

    2013-03-01

    Purpose: To report on early results of a single-institution phase 2 trial of a 5-fraction, once-weekly radiation therapy regimen for patients undergoing breast-conserving surgery (BCS). Methods and Materials: Patients who underwent BCS for American Joint Committee on Cancer stage 0, I, or II breast cancer with negative surgical margins were eligible to receive whole breast radiation therapy to a dose of 30 Gy in 5 weekly fractions of 6 Gy with or without an additional boost. Elective nodal irradiation was not permitted. There were no restrictions on breast size or the use of cytotoxic chemotherapy for otherwise eligible patients. Patients were assessed at baseline, treatment completion, and at first posttreatment follow-up to assess acute toxicity (Common Terminology Criteria for Adverse Events, version 3.0) and quality of life (European Organization for Research and Treatment of Cancer QLQ-BR23). Results: Between January and September 2011, 42 eligible patients underwent weekly hypofractionated breast irradiation immediately following BCS (69.0%) or at the conclusion of cytotoxic chemotherapy (31.0%). The rates of grade ?2 radiation-induced dermatitis, pain, fatigue, and breast edema were 19.0%, 11.9%, 9.5%, and 2.4%, respectively. Only 1 grade 3 toxicitypain requiring a course of narcotic analgesicswas observed. One patient developed a superficial cellulitis (grade 2), which resolved with the use of oral antibiotics. Patient-reported moderate-to-major breast symptoms (pain, swelling, and skin problems), all decreased from baseline through 1 month, whereas breast sensitivity remained stable over the study period. Conclusions: The tolerance of weekly hypofractionated breast irradiation compares well with recent reports of daily hypofractionated whole-breast irradiation schedules. The regimen appears feasible and cost-effective. Additional follow-up with continued accrual is needed to assess late toxicity, cosmesis, and disease-specific outcomes.

  5. Arsenic toxicity induced endothelial dysfunction and dementia: Pharmacological interdiction by histone deacetylase and inducible nitric oxide synthase inhibitors

    SciTech Connect (OSTI)

    Sharma, Bhupesh Sharma, P.M.

    2013-11-15

    Arsenic toxicity has been reported to damage all the major organs including the brain and vasculature. Dementia including Alzheimer's disease (AD) and vascular dementia (VaD) are posing greater risk to the world population as it is now increasing at a faster rate. We have investigated the role of sodium butyrate, a selective histone deacetylase (HDAC) inhibitor and aminoguanidine, a selective inducible nitric oxide synthase (iNOS) inhibitor in pharmacological interdiction of arsenic toxicity induced vascular endothelial dysfunction and dementia in rats. Arsenic toxicity was done by administering arsenic drinking water to rats. Morris water-maze (MWM) test was used for assessment of learning and memory. Endothelial function was assessed using student physiograph. Oxidative stress (aortic superoxide anion, serum and brain thiobarbituric acid reactive species, brain glutathione) and nitric oxide levels (serum nitrite/nitrate) were also measured. Arsenic treated rats have shown impairment of endothelial function, learning and memory, reduction in serum nitrite/nitrate and brain GSH levels along with increase in serum and brain TBARS. Sodium butyrate as well as aminoguanidine significantly convalesce arsenic induced impairment of learning, memory, endothelial function, and alterations in various biochemical parameters. It may be concluded that arsenic induces endothelial dysfunction and dementia, whereas, sodium butyrate, a HDAC inhibitor as well as aminoguanidine, a selective iNOS inhibitor may be considered as potential agents for the management of arsenic induced endothelial dysfunction and dementia. - Highlights: • As has induced endothelial dysfunction (Edf) and vascular dementia (VaD). • As has increased oxidative stress, AChE activity and decreased serum NO. • Inhibitors of HDAC and iNOS have attenuated As induced Edf and VaD. • Both the inhibitors have attenuated As induced biochemical changes. • Inhibitor of HDAC and iNOS has shown good potential in As induced VaD.

  6. Separation of toxic metal ions, hydrophilic hydrocarbons, hydrophobic fuel and halogenated hydrocarbons and recovery of ethanol from a process stream

    DOE Patents [OSTI]

    Kansa, Edward J. (Livermore, CA); Anderson, Brian L. (Lodi, CA); Wijesinghe, Ananda M. (Tracy, CA); Viani, Brian E. (Oakland, CA)

    1999-01-01

    This invention provides a process to tremendously reduce the bulk volume of contaminants obtained from an effluent stream produced subsurface remediation. The chemicals used for the subsurface remediation are reclaimed for recycling to the remediation process. Additional reductions in contaminant bulk volume are achieved by the ultra-violet light destruction of halogenated hydrocarbons, and the complete oxidation of hydrophobic fuel hydrocarbons and hydrophilic hydrocarbons. The contaminated bulk volume will arise primarily from the disposal of the toxic metal ions. The entire process is modular, so if there are any technological breakthroughs in one or more of the component process modules, such modules can be readily replaced.

  7. Air toxic emissions from the combustion of coal: Identifying and quantifying hazardous air pollutants from US coals

    SciTech Connect (OSTI)

    Szpunar, C.B.

    1992-09-01

    This report addresses the key air toxic emissions likely to emanate from continued and expanded use of domestic coal. It identifies and quantifies those trace elements specified in the US 1990 Clean Air Act Amendments, by tabulating selected characterization data on various source coals by region, state, and rank. On the basis of measurements by various researchers, this report also identifies those organic compounds likely to be derived from the coal combustion process (although their formation is highly dependent on specific boiler configurations and operating conditions).

  8. Separation of toxic metal ions, hydrophilic hydrocarbons, hydrophobic fuel and halogenated hydrocarbons and recovery of ethanol from a process stream

    DOE Patents [OSTI]

    Kansa, E.J.; Anderson, B.L.; Wijesinghe, A.M.; Viani, B.E.

    1999-05-25

    This invention provides a process to tremendously reduce the bulk volume of contaminants obtained from an effluent stream produced subsurface remediation. The chemicals used for the subsurface remediation are reclaimed for recycling to the remediation process. Additional reductions in contaminant bulk volume are achieved by the ultra-violet light destruction of halogenated hydrocarbons, and the complete oxidation of hydrophobic fuel hydrocarbons and hydrophilic hydrocarbons. The contaminated bulk volume will arise primarily from the disposal of the toxic metal ions. The entire process is modular, so if there are any technological breakthroughs in one or more of the component process modules, such modules can be readily replaced. 3 figs.

  9. A Novel Method for Predicting Late Genitourinary Toxicity After Prostate Radiation Therapy and the Need for Age-Based Risk-Adapted Dose Constraints

    SciTech Connect (OSTI)

    Ahmed, Awad A.; Egleston, Brian; Alcantara, Pino; Li, Linna; Pollack, Alan; Horwitz, Eric M.; Buyyounouski, Mark K.

    2013-07-15

    Background: There are no well-established normal tissue sparing dosevolume histogram (DVH) criteria that limit the risk of urinary toxicity from prostate radiation therapy (RT). The aim of this study was to determine which criteria predict late toxicity among various DVH parameters when contouring the entire solid bladder and its contents versus the bladder wall. The area under the histogram curve (AUHC) was also analyzed. Methods and Materials: From 1993 to 2000, 503 men with prostate cancer received 3-dimensional conformal RT (median follow-up time, 71 months). The whole bladder and the bladder wall were contoured in all patients. The primary endpoint was grade ?2 genitourinary (GU) toxicity occurring ?3 months after completion of RT. Cox regressions of time to grade ?2 toxicity were estimated separately for the entire bladder and bladder wall. Concordance probability estimates (CPE) assessed model discriminative ability. Before training the models, an external random test group of 100 men was set aside for testing. Separate analyses were performed based on the mean age (? 68 vs >68 years). Results: Age, pretreatment urinary symptoms, mean dose (entire bladder and bladder wall), and AUHC (entire bladder and bladder wall) were significant (P<.05) in multivariable analysis. Overall, bladder wall CPE values were higher than solid bladder values. The AUHC for bladder wall provided the greatest discrimination for late bladder toxicity when compared with alternative DVH points, with CPE values of 0.68 for age ?68 years and 0.81 for age >68 years. Conclusion: The AUHC method based on bladder wall volumes was superior for predicting late GU toxicity. Age >68 years was associated with late grade ?2 GU toxicity, which suggests that risk-adapted dose constraints based on age should be explored.

  10. Critical dose and toxicity index of organs at risk in radiotherapy: Analyzing the calculated effects of modified dose fractionation in nonsmall cell lung cancer

    SciTech Connect (OSTI)

    Pedicini, Piernicola; Strigari, Lidia; Benassi, Marcello; Caivano, Rocchina; Fiorentino, Alba; Nappi, Antonio; Salvatore, Marco; Storto, Giovanni

    2014-04-01

    To increase the efficacy of radiotherapy for nonsmall cell lung cancer (NSCLC), many schemes of dose fractionation were assessed by a new toxicity index (I), which allows one to choose the fractionation schedules that produce less toxic treatments. Thirty-two patients affected by non resectable NSCLC were treated by standard 3-dimensional conformal radiotherapy (3DCRT) with a strategy of limited treated volume. Computed tomography datasets were employed to re plan by simultaneous integrated boost intensity-modulated radiotherapy (IMRT). The dose distributions from plans were used to test various schemes of dose fractionation, in 3DCRT as well as in IMRT, by transforming the dose-volume histogram (DVH) into a biological equivalent DVH (BDVH) and by varying the overall treatment time. The BDVHs were obtained through the toxicity index, which was defined for each of the organs at risk (OAR) by a linear quadratic model keeping an equivalent radiobiological effect on the target volume. The less toxic fractionation consisted in a severe/moderate hyper fractionation for the volume including the primary tumor and lymph nodes, followed by a hypofractionation for the reduced volume of the primary tumor. The 3DCRT and IMRT resulted, respectively, in 4.7% and 4.3% of dose sparing for the spinal cord, without significant changes for the combined-lungs toxicity (p < 0.001). Schedules with reduced overall treatment time (accelerated fractionations) led to a 12.5% dose sparing for the spinal cord (7.5% in IMRT), 8.3% dose sparing for V{sub 20} in the combined lungs (5.5% in IMRT), and also significant dose sparing for all the other OARs (p < 0.001). The toxicity index allows to choose fractionation schedules with reduced toxicity for all the OARs and equivalent radiobiological effect for the tumor in 3DCRT, as well as in IMRT, treatments of NSCLC.

  11. Prospective Study of Local Control and Late Radiation Toxicity After Intraoperative Radiation Therapy Boost for Early Breast Cancer

    SciTech Connect (OSTI)

    Chang, David W.; Marvelde, Luc te; Chua, Boon H.

    2014-01-01

    Purpose: To report the local recurrence rate and late toxicity of intraoperative radiation therapy (IORT) boost to the tumor bed using the Intrabeam System followed by external-beam whole-breast irradiation (WBI) in women with early-stage breast cancer in a prospective single-institution study. Methods and Materials: Women with breast cancer ?3 cm were recruited between February 2003 and May 2005. After breast-conserving surgery, a single dose of 5 Gy IORT boost was delivered using 50-kV x-rays to a depth of 10 mm from the applicator surface. This was followed by WBI to a total dose of 50 Gy in 25 fractions. Patients were reviewed at regular, predefined intervals. Late toxicities were recorded using the Radiation Therapy Oncology Group/European Organization for Research and Treatment of Cancer Late Radiation Morbidity Scoring systems. Results: Fifty-five patients completed both IORT boost and external-beam WBI. Median follow-up was 3.3 years (range, 1.4-4.1 years). There was no reported locoregional recurrence or death. One patient developed distant metastases. Grade 2 and 3 subcutaneous fibrosis was detected in 29 (53%) and 8 patients (15%), respectively. Conclusions: The use of IORT as a tumor bed boost using kV x-rays in breast-conserving therapy was associated with good local control but a clinically significant rate of grade 2 and 3 subcutaneous fibrosis.

  12. Use of genomic data in risk assessment case study: I. Evaluation of the dibutyl phthalate male reproductive development toxicity data set

    SciTech Connect (OSTI)

    Makris, Susan L.; Euling, Susan Y.; Gray, L. Earl; Benson, Robert; Foster, Paul M.D.

    2013-09-15

    A case study was conducted, using dibutyl phthalate (DBP), to explore an approach to using toxicogenomic data in risk assessment. The toxicity and toxicogenomic data sets relative to DBP-related male reproductive developmental outcomes were considered conjointly to derive information about mode and mechanism of action. In this manuscript, we describe the case study evaluation of the toxicological database for DBP, focusing on identifying the full spectrum of male reproductive developmental effects. The data were assessed to 1) evaluate low dose and low incidence findings and 2) identify male reproductive toxicity endpoints without well-established modes of action (MOAs). These efforts led to the characterization of data gaps and research needs for the toxicity and toxicogenomic studies in a risk assessment context. Further, the identification of endpoints with unexplained MOAs in the toxicity data set was useful in the subsequent evaluation of the mechanistic information that the toxicogenomic data set evaluation could provide. The extensive analysis of the toxicology data set within the MOA context provided a resource of information for DBP in attempts to hypothesize MOAs (for endpoints without a well-established MOA) and to phenotypically anchor toxicogenomic and other mechanistic data both to toxicity endpoints and to available toxicogenomic data. This case study serves as an example of the steps that can be taken to develop a toxicological data source for a risk assessment, both in general and especially for risk assessments that include toxicogenomic data.

  13. 1997 Toxic Chemical Release Inventory Report for the Emergency Planning and Community Right-to-Know Act of 1986, Title III, Section 313

    SciTech Connect (OSTI)

    Heather McBride

    1997-07-01

    The Emergency Planning and Community Right-to-Know Act of 1986 (EPCIL4), Title III, Section 313 [also known as the Superfund Amendment and Reauthorization Act (SARA)], as modified by Executive Order 12856, requires all federal facilities to submit an annual Toxic Chemical Release Inventory report every July for the preceding calendar year. Owners and operators of manufacturing, processing, or production facilities are required to report their toxic chemical releases to all environmental mediums (air, water, soil, etc.). At Los Alamos National Laboratory (LANL), nitric acid was the only toxic chemical used in 1997 that met the reportable threshold limit of 10,000 lb. Form R is the only documentation required by the Environmental Protection Agency, and it is included in the appendix of this report. This report, as requested by DOE, is provided for documentation purposes. In addition, a detailed description of the evaluation and reporting process for chemicals and processes at LANL has been included.

  14. substance-abuse-policy.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  15. Predictors of Grade 3 or Higher Late Bowel Toxicity in Patients Undergoing Pelvic Radiation for Cervical Cancer: Results From a Prospective Study

    SciTech Connect (OSTI)

    Chopra, Supriya; Dora, Tapas; Chinnachamy, Anand N.; Thomas, Biji; Kannan, Sadhna; Engineer, Reena; Mahantshetty, Umesh; Phurailatpam, Reena; Paul, Siji N.; Shrivastava, Shyam Kishore

    2014-03-01

    Purpose: The present study investigates relationship between dosevolume parameters and severe bowel toxicity after postoperative radiation treatment (PORT) for cervical cancer. Methods and Materials: From June 2010 to December 2012, a total of 71 patients undergoing PORT were included. Small bowel (SB) and large bowel (LB) loops were contoured 2cm above the target volume. The volume of SB and LB that received 15Gy, 30Gy, and 40Gy was calculated (V15 SB, V15 LB, V30 SB, V30 LB, V40 SB, V 40 LB). On follow-up, bowel toxicity was scored using Common Terminology Criteria for Adverse Events (CTCAE), version 3.0. A reciever operating characteristic (ROC) curve identified volume thresholds that predicted for grade 3 or higher toxicity with highest specificity. All data was dichotomized across these identified cut-off values. Univariate and multivariate analysis was performed using SPSS, version15. Results: The median patient age was 47years (range, 35-65years). Of the 71 patients, 46 received image-guided intensity modulated radiation therapy, and 25 received conformal radiation (50Gy in 25 fractions for 5weeks). Overall, 63 of 71 patients received concurrent chemotherapy. On a median follow-up of 18months (range, 8-29months), grade 2 or higher bowel toxicity was seen in 22 of 71 patients (30.9%) and grade 3 or higher bowel toxicity was seen in 9 patients (12.6%). On univariate analysis, V15 SB <275 cc (P=.01), V30 SB <190 cc (P=.02), V40 SB <150 cc (P=.01), and V15 LB <250 cc (P=.03), and V40 LB <90 cc (P=.04) predicted for absence of grade 3 or higher toxicity. No other patient- or treatment-related factors were statistically significant. On multivariate analysis, only V15 SB (P=.002) and V15 LB (P=.03) were statistically significant. Conclusions: V 15Gy SB and LB are independent predictors of late grade 3 or higher toxicity. Restricting V15 SB and V15 LB to <275 cc and <250 cc can reduce grade 3 or higher toxicity to less than 5%.

  16. Reduced Toxicity With Intensity Modulated Radiation Therapy (IMRT) for Desmoplastic Small Round Cell Tumor (DSRCT): An Update on the Whole Abdominopelvic Radiation Therapy (WAP-RT) Experience

    SciTech Connect (OSTI)

    Desai, Neil B.; Stein, Nicholas F.; LaQuaglia, Michael P.; Alektiar, Kaled M.; Kushner, Brian H.; Modak, Shakeel; Magnan, Heather M.; Goodman, Karyn; Wolden, Suzanne L.

    2013-01-01

    Purpose: Desmoplastic small round cell tumor (DSRCT) is a rare malignancy typically involving the peritoneum in young men. Whole abdominopelvic radiation therapy (WAP-RT) using conventional 2-dimensional (2D) radiation therapy (RT) is used to address local recurrence but has been limited by toxicity. Our objectives were to assess the benefit of intensity modulated radiation therapy (IMRT) on toxicity and to update the largest series on radiation for DSRCT. Methods and Materials: The records of 31 patients with DSRCT treated with WAP-RT (22 with 2D-RT and 9 with IMRT) between 1992 and 2011 were retrospectively reviewed. All received multi-agent chemotherapy and maximal surgical debulking followed by 30 Gy of WAP-RT. A further focal boost of 12 to 24 Gy was used in 12 cases. Boost RT and autologous stem cell transplantation were nearly exclusive to patients treated with 2D-RT. Toxicities were assessed with the Common Terminology Criteria for Adverse Events. Dosimetric analysis compared IMRT and simulated 2D-RT dose distributions. Results: Of 31 patients, 30 completed WAP-RT, with a median follow-up after RT of 19 months. Acute toxicity was reduced with IMRT versus 2D-RT: P=.04 for gastrointestinal toxicity of grade 2 or higher (33% vs 77%); P=.02 for grade 4 hematologic toxicity (33% vs 86%); P=.01 for rates of granulocyte colony-stimulating factor; and P=.04 for rates of platelet transfusion. Post treatment red blood cell and platelet transfusion rates were also reduced (P=.01). IMRT improved target homogeneity ([D05-D95]/D05 of 21% vs 46%) and resulted in a 21% mean bone dose reduction. Small bowel obstruction was the most common late toxicity (23% overall). Updated 3-year overall survival and progression-free survival rates were 50% and 24%, respectively. Overall survival was associated with distant metastasis at diagnosis on multivariate analysis. Most failures remained intraperitoneal (88%). Conclusions: IMRT for consolidative WAP-RT in DSRCT improves hematologic toxicity in particular. Although the long-term efficacy of current treatment options remains disappointing, the improved therapeutic index of IMRT may aid in generalizing its use and allowing the addition of novel approaches such as intraperitoneal immunotherapy.

  17. Gender-specific reduction of hepatic Mrp2 expression by high-fat diet protects female mice from ANIT toxicity

    SciTech Connect (OSTI)

    Kong, Bo; Csanaky, Ivn L.; Aleksunes, Lauren M.; Patni, Meghan; Chen, Qi; Ma, Xiaochao; Jaeschke, Hartmut; Weir, Scott; Broward, Melinda; Klaassen, Curtis D.; University of Kansas Cancer Center, Kansas City, KS ; Guo, Grace L.

    2012-06-01

    Emerging evidence suggests that feeding a high-fat diet (HFD) to rodents affects the expression of genes involved in drug transport. However, gender-specific effects of HFD on drug transport are not known. The multidrug resistance-associated protein 2 (Mrp2, Abcc2) is a transporter highly expressed in the hepatocyte canalicular membrane and is important for biliary excretion of glutathione-conjugated chemicals. The current study showed that hepatic Mrp2 expression was reduced by HFD feeding only in female, but not male, C57BL/6J mice. In order to determine whether down-regulation of Mrp2 in female mice altered chemical disposition and toxicity, the biliary excretion and hepatotoxicity of the Mrp2 substrate, ?-naphthylisothiocyanate (ANIT), were assessed in male and female mice fed control diet or HFD for 4 weeks. ANIT-induced biliary injury is a commonly used model of experimental cholestasis and has been shown to be dependent upon Mrp2-mediated efflux of an ANIT glutathione conjugate that selectively injures biliary epithelial cells. Interestingly, HFD feeding significantly reduced early-phase biliary ANIT excretion in female mice and largely protected against ANIT-induced liver injury. In summary, the current study showed that, at least in mice, HFD feeding can differentially regulate Mrp2 expression and function and depending upon the chemical exposure may enhance or reduce susceptibility to toxicity. Taken together, these data provide a novel interaction between diet and gender in regulating hepatobiliary excretion and susceptibility to injury. -- Highlights: ? High-fat diet decreases hepatic Mrp2 expression only in female but not in male mice. ? HFD significantly reduces early-phase biliary ANIT excretion in female mice. ? HFD protects female mice against ANIT-induced liver injury.

  18. Corrective Action Investigation Plan for Corrective Action Unit 552: Area 12 Muckpile and Ponds, Nevada Test Site, Nevada, Rev. 1

    SciTech Connect (OSTI)

    Robert F. Boehlecke

    2005-01-01

    Corrective Action Unit 552 is being investigated because man-made radionuclides and chemical contaminants may be present in concentrations that could potentially pose an unacceptable risk to human health and/or the environment. The CAI will be conducted following the data quality objectives (DQOs) developed by representatives of the Nevada Division of Environmental Protection (NDEP) and the DOE National Nuclear Security Administration Nevada Site Office (NNSA/NSO). The DQOs are used to identify the type, amount, and quality of data needed to define the nature and extent of contamination and identify and evaluate the most appropriate corrective action alternatives for CAU 552. The primary problem statement for the investigation is: ''Existing information on the nature and extent of potential contamination is insufficient to evaluate and recommend corrective action alternatives for CAS 12-23-05.'' To address this problem statement, the resolution of the following two decision statements is required: (1) The Decision I statement is: ''Is a contaminant present within the CAU at a concentration that could pose an unacceptable risk to human health and the environment?'' Any site-related contaminant detected at a concentration exceeding the corresponding preliminary action level (PAL), as defined in Section A.1.4.2, will be considered a contaminant of concern (COC). A COC is defined as a site-related constituent that exceeds the screening criteria (PAL). The presence of a contaminant within each CAS is defined as the analytical detection of a COC. (2) The Decision II statement is: ''Determine the extent of contamination identified above PALs.'' This decision will be achieved by the collection of data that are adequate to define the extent of COCs. Decision II samples are used to determine the lateral and vertical extent of the contamination as well as the likelihood of COCs to migrate outside of the site boundaries. The migration pattern can be derived from the Decision II samples, since the analytical results of those samples will show how far the contamination has travelled in the time period since activities at the site ended. Most of the data necessary to resolve the decisions will be generated from the analysis of environmental samples collected during the CAI for CAU 552. The general purpose of the investigation is to: (1) Identify the presence and nature of COCs. (2) Determine the vertical and lateral extent of identified COCs. (3) Ensure sufficient data is collected to support the selection of a corrective action compliant with all NDEP, ''Resource Conservation and Recovery Act (RCRA), Toxic Substance Control Act (TSCA)'', and DOE requirements. In addition, data will be obtained to support (IDW) disposal and potential future waste management decisions.

  19. Application for Permit to Operate a Class II Solid Waste Disposal Site at the Nevada Test Site - U10c Disposal Site

    SciTech Connect (OSTI)

    NSTec Environmental Programs

    2010-03-31

    The Nevada Test Site (NTS) is located approximately 105 km (65 mi) northwest of Las Vegas, Nevada. National Nuclear Security Administration Nevada Site Office (NNSA/NSO) is the federal lands management authority for the NTS and National Security Technologies LLC (NSTec) is the Management and Operations contractor. Access on and off the NTS is tightly controlled, restricted, and guarded on a 24-hour basis. The NTS is posted with signs along its entire perimeter. NSTec is the operator of all solid waste disposal sites on the NTS. The site will be used for the disposal of refuse, rubbish, garbage, sewage sludge, pathological waste, Asbestos-Containing Material (ACM), industrial solid waste, hydrocarbon-burdened soil, hydrocarbon-burdened demolition and construction waste, and other inert waste (hereafter called permissible waste). Waste containing free liquids or regulated under Subtitle C of the Resource Conservation and Recovery Act (RCRA) will not be accepted for disposal at the site. Waste regulated under the Toxic Substance Control Act (TSCA), excluding Polychlorinated Biphenyl [PCB], Bulk Product Waste (see Section 6.2.5) and ACM (see Section 6.2.2.2) will not be accepted for disposal at the site. The disposal site will be used as the sole depository of permissible waste which is: (1) Generated by entities covered under the U.S. Environmental Protection Agency (EPA) Hazardous Waste Generator Identification Number for the NTS; (2) Generated at sites identified in the Federal Facilities Agreement and Consent Order (FFACO); (3) Sensitive records and media, including documents, vugraphs, computer disks, typewriter ribbons, magnetic tapes, etc., generated by NNSA/NSO or its contractors; (4) ACM generated by NNSA/NSO or its contractors according to Section 6.2.2.2, as necessary; (5) Hydrocarbon-burdened soil and solid waste from areas covered under the EPA Hazardous Waste Generator Identification Number for the NTS; (6) Other waste on a case-by-case concurrence by NDEP/BFF. The generator of permissible waste is responsible for preparing documentation related to waste acceptance criteria, waste characterization, and load verification. Waste and Water (WW) personnel are responsible for operating the disposal site and reviewing documentation to determine if the waste is acceptable.

  20. 1998 Toxic Chemical Release Inventory Report for the Emergency Planning and Community Right-to-Know Act of 1986, Title III

    SciTech Connect (OSTI)

    Marjorie B. Stockton

    1999-11-01

    The Emergency Planning and Community Right-to-Know Act (EPCRA) of 1986 [also known as the Superfund Amendment and Reauthorization Act (SARA), Title III], as modified by Executive Order 12856, requires that all federal facilities evaluate the need to submit an annual Toxic Chemical Release Inventory report as prescribed in Title III, Section 313 of this Act. This annual report is due every July for the preceding calendar year. Owners and operators who manufacture, process, or otherwise use certain toxic chemicals above listed threshold quantities are required to report their toxic chemical releases to all environmental mediums (air, water, soil, etc.). At Los Alamos National Laboratory (LANL), no EPCRA Section 313 chemicals were used in 1998 above the reportable threshold limits of 10,000 lb or 25,000 lb. Therefore LANL was not required to submit any Toxic Chemical Release Inventory reports (Form Rs) for 1998. This document was prepared to provide a detailed description of the evaluation on chemical usage and EPCRA Section 313 threshold determinations for LANL for 1998.

  1. 2002 Toxic Chemical Release Inventory Report for the Emergency Planning and Community Right-to-Know Act of 1986, Title III, Section 313

    SciTech Connect (OSTI)

    M. Stockton

    2003-11-01

    For reporting year 2002, Los Alamos National Laboratory (LANL or the Laboratory) submitted Form R reports for lead compounds and mercury as required under the Emergency Planning and Community Right-to-Know Act (EPCRA), Section 313. No other EPCRA Section 313 chemicals were used in 2002 above the reportable thresholds. This document was prepared to provide a description of the evaluation of EPCRA Section 313 chemical usage and threshold determinations for LANL for calendar year 2002 as well as provide background information about the data included on the Form R reports. Section 313 of EPCRA specifically requires facilities to submit a Toxic Chemical Release Inventory report (Form R) to the U.S. Environmental Protection Agency (EPA) and state agencies if the owners and operators manufacture, process, or otherwise use any of the listed toxic chemicals above listed threshold quantities. EPA compiles this data in the Toxic Release Inventory database. Form R reports for each chemical over threshold quantities must be submitted on or before July 1 each year and must cover activities that occurred at the facility during the previous year. In 1999 EPA promulgated a final rule on Persistent Bioaccumulative Toxics (PBTs). This rule added several chemicals to the EPCRA Section 313 list of toxic chemicals and established lower reporting thresholds for these and other PBT chemicals that were already reportable under EPCRA Section 313. These lower thresholds became applicable in reporting year 2000. In 2001, EPA expanded the PBT rule to include a lower reporting threshold for lead and lead compounds. Facilities that manufacture, process, or otherwise use more than 100 lb of lead or lead compounds must submit a Form R.

  2. 2006 Toxic Chemical Release Inventory Report for the Emergency Planning and Community Right-to-Know Act of 1986, Title III, Section 313

    SciTech Connect (OSTI)

    Ecology and Air Quality Group (ENV-EAQ)

    2007-12-12

    For reporting year 2006, Los Alamos National Laboratory (LANL or the Laboratory) submitted Form R reports for lead as required under the Emergency Planning and Community Right-to-Know Act (EPCRA) Section 313. No other EPCRA Section 313 chemicals were used in 2006 above the reportable thresholds. This document was prepared to provide a description of the evaluation of EPCRA Section 313 chemical use and threshold determinations for LANL for calendar year 2006, as well as to provide background information about data included on the Form R reports. Section 313 of EPCRA specifically requires facilities to submit a Toxic Chemical Release Inventory Report (Form R) to the U.S. Environmental Protection Agency (EPA) and state agencies if the owners and operators manufacture, process, or otherwise use any of the listed toxic chemicals above listed threshold quantities. EPA compiles this data in the Toxic Release Inventory database. Form R reports for each chemical over threshold quantities must be submitted on or before July 1 each year and must cover activities that occurred at the facility during the previous year. In 1999, EPA promulgated a final rule on persistent bioaccumulative toxics (PBTs). This rule added several chemicals to the EPCRA Section 313 list of toxic chemicals and established lower reporting thresholds for these and other PBT chemicals that were already reportable. These lower thresholds became applicable in reporting year 2000. In 2001, EPA expanded the PBT rule to include a lower reporting threshold for lead and lead compounds. Facilities that manufacture, process, or otherwise use more than 100 lb of lead or lead compounds must submit a Form R.

  3. Waste Isolation Pilot Plant Initial Report for PCB Disposal Authorization (40 CFR {section} 761.75[c])

    SciTech Connect (OSTI)

    Westinghouse TRU Solutions

    2002-03-19

    This initial report is being submitted pursuant to Title 40 Code of Federal Regulations (CFR) {section} 761.75(c) to request authorization to allow the disposal of transuranic (TRU) wastes containing polychlorinated biphenyls (PCBs) which are duly regulated under the Toxic Substances Control Act (TSCA). Approval of this initial report will not affect the disposal of TRU or TRU mixed wastes that do not contain PCBs. This initial report also demonstrates how the Waste Isolation Pilot Plant (WIPP) meets or exceeds the technical standards for a Chemical Waste Landfill. Approval of this request will allow the U.S. Department of Energy (DOE) to dispose of approximately 88,000 cubic feet (ft3) (2,500 cubic meters [m3]) of TRU wastes containing PCBs subject to regulation under the TSCA. This approval will include only those PCB/TRU wastes, which the TSCA regulations allow for disposal of the PCB component in municipal solid waste facilities or chemical waste landfills (e.g., PCB remediation waste, PC B articles, and bulk PCB product waste). Disposal of TRU waste by the DOE is congressionally mandated in Public Law 102-579 (as amended by the National Defense Authorization Act for Fiscal Year 1997, Pub. L. 104-201, referred to as the WIPP Land Withdrawal Act [LWA]). Portions of the TRU waste inventory contain hazardous waste constituents regulated under 40 CFR Parts 260 through 279, and/or PCBs and PCB Items regulated under 40 CFR Part 761. Therefore, the DOE TRU waste program must address the disposal requirements for these hazardous waste constituents and PCBs. To facilitate the disposal of TRU wastes containing hazardous waste constituents, the owner/operators received a Hazardous Waste Facility Permit (HWFP) from the New Mexico Environment Department (NMED) on October 27, 1999. The permit allows the disposal of TRU wastes subject to hazardous waste disposal requirements (TRU mixed waste). Informational copies of this permit and other referenced documents are available from the WIPP website. To facilitate the disposal of TRU wastes containing PCBs, the owner/operators are hereby submitting this initial report containing information required pursuant to the Chemical Waste Landfill Approval requirements in 40 CFR {section} 761.75(c). Although WIPP is defined as a miscellaneous unit and not a landfill by the New Mexico Hazardous Waste Act, WIPP meets or exceeds all applicable technical standards for chemical waste landfills by virtue of its design and programs as indicated in the Engineering Report (Attachment B). The layout of this initial report is consistent with requirements (i.e., Sections 2.0 through 12.0 following the sequence of 40 CFR {section} 761.75[c][i] -[ix] with sections added to discuss the Contingency and Training Plans; and Attachment B of this initial report addresses the requirements of 40 CFR {section} 761.75[b][1] through [9] in this order). This initial report includes a description of three proposed changes that will be subject to ''conditional approval.'' The first will allow the disposal of remote-handled (RH) PCB/TRU waste at WIPP. The second will allow the establishment of a central confirmation facility at WIPP. The third will allow for an increase in contact-handled Working Copy Waste Isolation Pilot Plant Initial Report for PCB Disposal Authorization DOE/WIPP 02-3196 (CH) waste storage capacities. These proposed changes are discussed further in Section 3.3 of this initial report. ''Conditional approval'' of these requests would allow these activities at WIPP contingent upon: - Approval of the HWFP modification (NMED) and Compliance Certification Application (CCA) change request (Environmental Protection Agency [EPA]) - Inspection of facility prior to implementing the change (if deemed necessary by the EPA) - Written approval from the EPA This initial report also includes the following three requests for waivers to the technical requirements for Chemical Waste Landfills pursuant to 40 CFR {section} 761.75(c)(4): - Hydrologic Conditions (40 CFR {section} 761.75[b][3]) - Monitoring Systems (40 CFR {sect

  4. Projections of air toxic emissions from coal-fired utility combustion: Input for hazardous air pollutant regulators

    SciTech Connect (OSTI)

    Szpunar, C.B.

    1993-08-01

    The US Environmental Protection Agency (EPA) is required by the 1990 CAAA to promulgate rules for all ``major`` sources of any of these HAPs. According to the HAPs section of the new Title III, any stationary source emitting 10 tons per year (TPY) of one HAP or 25 TPY of a combination of HAPs will be considered and designated a major source. In contrast to the original National Emission Standards for Hazardous Air Pollutants (NESHAP), which were designed to protect public health to ``an ample margin of safety,`` the new Title III, in its first phase, will regulate by industrial category those sources emitting HAPs in excess of the 10/25-TPY threshold levels, regardless of health risks. The trace elements normally associated with coal mineral matter and the various compounds formed during coal combustion have the potential to produce hazardous air toxic emissions from coal-fired electric utilities. Under Title III, the EPA is required to perform certain studies, prior to any regulation of electric utilities; these studies are currently underway. Also, the US Department of Energy (DOE) maintains a vested interest in addressing those energy policy questions affecting electric utility generation, coal mining, and steel producing critical to this country`s economic well-being, where balancing the costs to the producers and users of energy with the benefits of environmental protection to the workers and the general populace remains of significant concern.

  5. Technical evaluation of topsoil substitution practices and handling of potential acid/toxic-forming materials in Texas. Special study report

    SciTech Connect (OSTI)

    Not Available

    1985-09-01

    The Texas State program approved by the Office of Surface Mining (OSM) under the Surface Mining Control and Reclamation Act of 1977 (SMCRA) allows that selected overburden materials may, if justified, be substituted for topsoil in mined land reclamation. The report presents the Office of Surface Mining Reclamation and Enforcement's (OSM's) findings regarding the practice of topsoil substitution under the approved Texas program and related reclamation problems with potential minesoil acidification. The purpose of the study was not to determine whether the substitution of overburden for topsoil should be approved or disapproved on any specific mine or soil series in Texas. The report presents a summary of pertinent technical considerations that need to be addressed in permit approvals for surface coal mines which (1) may encounter potentially acid/toxic-forming materials during mining or (2) intend to substitute overburden for topsoil as a plant growth material. The report summarizes the results of a special study OSM conducted to evaluate the technical basis and justification for reclamation plans and the substitution of overburden for topsoil as a plant growth material suitable for the reclamation of coal mines.

  6. Advances in exposure and toxicity assessment of particulate matter: An overview of presentations at the 2009 Toxicology and Risk Assessment Conference

    SciTech Connect (OSTI)

    Gunasekar, Palur G.; Stanek, Lindsay W.

    2011-07-15

    The 2009 Toxicology and Risk Assessment Conference (TRAC) session on 'Advances in Exposure and Toxicity Assessment of Particulate Matter' was held in April 2009 in West Chester, OH. The goal of this session was to bring together toxicology, geology and risk assessment experts from the Department of Defense and academia to examine issues in exposure assessment and report on recent epidemiological findings of health effects associated with particulate matter (PM) exposure. Important aspects of PM exposure research are to detect and monitor low levels of PM with various chemical compositions and to assess the health risks associated with these exposures. As part of the overall theme, some presenters discussed collection methods for sand and dust from Iraqi and Afghanistan regions, health issues among deployed personnel, and future directions for risk assessment research among these populations. The remaining speakers focused on the toxicity of ultrafine PM and the characterization of aerosols generated during ballistic impacts of tungsten heavy alloys.

  7. Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenal

    SciTech Connect (OSTI)

    Tsirulnikov, Kirill; Abuladze, Natalia; Bragin, Anatol; Brain Research Institute, University of California at Los Angeles, CA 90095 ; Faull, Kym; Department of Psychiatry and Biobehavioral Sciences, University of California at Los Angeles, CA 90095; Pasarow Mass Spectrometry Laboratory, University of California at Los Angeles, CA 90095 ; Cascio, Duilio; Damoiseaux, Robert; Schibler, Matthew J.; Pushkin, Alexander

    2012-09-15

    4-Hydroxy-2-nonenal (4HNE) and acrolein (ACR) are highly reactive neurotoxic products of lipid peroxidation that are implicated in the pathogenesis and progression of Alzheimer's and Parkinson's diseases. Conjugation with glutathione (GSH) initiates the 4HNE and ACR detoxification pathway, which generates the mercapturates of 4HNE and ACR that can be excreted. Prior work has shown that the efficiency of the GSH-dependent renal detoxification of haloalkene derived mercapturates is significantly decreased upon their deacetylation because of rapid transformation of the deacetylated products into toxic compounds mediated by ?-lyase. The enzymes of the GSH-conjugation pathway and ?-lyases are expressed in the brain, and we hypothesized that a similar toxicity mechanism may be initiated in the brain by the deacetylation of 4HNE- and ACR-mercapturate. The present study was performed to identify an enzyme(s) involved in 4HNE- and ACR-mercapturate deacetylation, characterize the brain expression of this enzyme and determine whether its inhibition decreases 4HNE and 4HNE-mercapturate neurotoxicity. We demonstrated that of two candidate deacetylases, aminoacylases 1 (AA1) and 3 (AA3), only AA3 efficiently deacetylates both 4HNE- and ACR-mercapturate. AA3 was further localized to neurons and blood vessels. Using a small molecule screen we generated high-affinity AA3 inhibitors. Two of them completely protected rat brain cortex neurons expressing AA3 from the toxicity of 4HNE-mercapturate. 4HNE-cysteine (4HNE-Cys) was also neurotoxic and its toxicity was mostly prevented by a ?-lyase inhibitor, aminooxyacetate. The results suggest that the AA3 mediated deacetylation of 4HNE-mercapturate may be involved in the neurotoxicity of 4HNE.

  8. TOXICITY CHARACTERISTIC LEACHING PROCEDURE APPLIED TO RADIOACTIVE SALTSTONE CONTAINING TETRAPHENYLBORATE: DEVELOPMENT OF A MODIFIED ZERO-HEADSPACE EXTRACTOR

    SciTech Connect (OSTI)

    Crapse, K.; Cozzi, A.; Crawford, C.; Jurgensen, A.

    2006-09-30

    In order to assess the effect of extended curing times at elevated temperatures on saltstone containing Tank 48H waste, saltstone samples prepared as a part of a separate study were analyzed for benzene using a modification of the United States Environmental Protection Agency (USEPA) method 1311 Toxicity Characteristic Leaching Procedure (TCLP). To carry out TCLP for volatile organic analytes (VOA), such as benzene, in the Savannah River National Laboratory (SRNL) shielded cells (SC), a modified TCLP Zero-Headspace Extractor (ZHE) was developed. The modified method was demonstrated to be acceptable in a side by side comparison with an EPA recommended ZHE using nonradioactive saltstone containing tetraphenylborate (TPB). TCLP results for all saltstone samples tested containing TPB (both simulant and actual Tank 48H waste) were below the regulatory limit for benzene (0.5 mg/L). In general, higher curing temperatures corresponded to higher concentrations of benzene in TCLP extract. The TCLP performed on the simulant samples cured under the most extreme conditions (3000 mg/L TPB in salt and cured at 95 C for at least 144 days) resulted in benzene values that were greater than half the regulatory limit. Taking into account that benzene in TCLP extract was measured on the same order of magnitude as the regulatory limit, that these experimental conditions may not be representative of actual curing profiles found in the saltstone vault and that there is significant uncertainty associated with the precision of the method, it is recommended that to increase confidence in TCLP results for benzene, the maximum curing temperature of saltstone be less than 95 C. At this time, no further benzene TCLP testing is warranted. Additional verification would be recommended, however, should future processing strategies result in significant changes to salt waste composition in saltstone as factors beyond the scope of this limited study may influence the decomposition of TPB in saltstone.

  9. Resources for global risk assessment: The International Toxicity Estimates for Risk (ITER) and Risk Information Exchange (RiskIE) databases

    SciTech Connect (OSTI)

    Wullenweber, Andrea Kroner, Oliver; Kohrman, Melissa; Maier, Andrew; Dourson, Michael; Rak, Andrew; Wexler, Philip; Tomljanovic, Chuck

    2008-11-15

    The rate of chemical synthesis and use has outpaced the development of risk values and the resolution of risk assessment methodology questions. In addition, available risk values derived by different organizations may vary due to scientific judgments, mission of the organization, or use of more recently published data. Further, each organization derives values for a unique chemical list so it can be challenging to locate data on a given chemical. Two Internet resources are available to address these issues. First, the International Toxicity Estimates for Risk (ITER) database ( (www.tera.org/iter)) provides chronic human health risk assessment data from a variety of organizations worldwide in a side-by-side format, explains differences in risk values derived by different organizations, and links directly to each organization's website for more detailed information. It is also the only database that includes risk information from independent parties whose risk values have undergone independent peer review. Second, the Risk Information Exchange (RiskIE) is a database of in progress chemical risk assessment work, and includes non-chemical information related to human health risk assessment, such as training modules, white papers and risk documents. RiskIE is available at (http://www.allianceforrisk.org/RiskIE.htm), and will join ITER on National Library of Medicine's TOXNET ( (http://toxnet.nlm.nih.gov/)). Together, ITER and RiskIE provide risk assessors essential tools for easily identifying and comparing available risk data, for sharing in progress assessments, and for enhancing interaction among risk assessment groups to decrease duplication of effort and to harmonize risk assessment procedures across organizations.

  10. 2004 Toxic Chemical Release Inventory Report for the Emergency Planning and Community Right-to-Know Act of 1986, Title III, Section 313

    SciTech Connect (OSTI)

    M. Stockton

    2006-01-15

    Section 313 of Emergency Planning and Community Right-to-Know Act (EPCRA) specifically requires facilities to submit a Toxic Chemical Release Inventory Report (Form R) to the U.S. Environmental Protection Agency (EPA) and state agencies if the owners and operators manufacture, process, or otherwise use any of the listed toxic chemicals above listed threshold quantities. EPA compiles this data in the Toxic Release Inventory database. Form R reports for each chemical over threshold quantities must be submitted on or before July 1 each year and must cover activities that occurred at the facility during the previous year. For reporting year 2004, Los Alamos National Laboratory (LANL or the Laboratory) submitted Form R reports for lead compounds, nitric acid, and nitrate compounds as required under the EPCRA Section 313. No other EPCRA Section 313 chemicals were used in 2004 above the reportable thresholds. This document provides a description of the evaluation of EPCRA Section 313 chemical use and threshold determinations for LANL for calendar year 2004, as well as background information about data included on the Form R reports.

  11. Sun light mediated synthesis of gold nanoparticles as carrier for 6-mercaptopurine: Preparation, characterization and toxicity studies in zebrafish embryo model

    SciTech Connect (OSTI)

    Ganeshkumar, Moorthy; Sastry, Thotapalli Parvathaleswara; Sathish Kumar, Muniram; Dinesh, Murugan Girija; Kannappan, Sudalyandi; Suguna, Lonchin

    2012-09-15

    Highlights: ? Gold nanoparticles prepared using eco-friendly method with good in vitro stability. ? Can be used as drug delivery system. ? Did not show any toxicity in zebrafish embryo. ? More toxic to cancer cells when compared to N-Au-Mp and Mp. -- Abstract: The objective of this study is to synthesize green chemistry based gold nanoparticles by sun light irradiation method. The prepared gold nanoparticles (AuNPs) were modified using folic acid and then coupled with 6-mercaptopurine. These modified nanoparticles were used as a tool for targeted drug delivery to treat laryngeal cancer. In the present study, novel bionanocomposites containing nutrient agar coated gold nano particles (N-AuNPs) coupled with 6-mercaptopurine (drug) (N-AuNPs-Mp), folic acid (ligand) (N-AuNPs-Mp-Fa) and rhodamine (dye) (N-AuNPs-Rd), a fluorescent agent, were prepared and characterized by IR, UV, TEM, Particle size analysis and in vitro stability. The toxicity and fluorescence of N-Au was studied using zebrafish embryo model. The in vitro cytotoxicity of free Mp, N-Au-Mp and N-Au-Mp-Fa against HEp-2 cells was compared and found that the amount of Mp required to achieve 50% of growth of inhibition (IC{sub 50}) was much lower in N-Au-Mp-Fa than in free Mp and N-Au-Mp.

  12. Improving in vitro Sertoli cell/gonocyte co-culture model for assessing male reproductive toxicity: Lessons learned from comparisons of cytotoxicity versus genomic responses to phthalates

    SciTech Connect (OSTI)

    Yu Xiaozhong; Hong, Sung Woo; Moreira, Estefania G.; Faustman, Elaine M.

    2009-09-15

    Gonocytes exist in the neonatal testis and represent a transient population of male germ-line stem cells. It has been shown that stem cell self-renewal and progeny production is probably controlled by the neighboring differentiated cells and extracellular matrix (ECM) in vivo known as niches. Recently, we developed an in vitro three-dimensional (3D) Sertoli cell/gonocyte co-culture (SGC) model with ECM overlay, which creates an in vivo-like niche and supports germ-line stem cell functioning within a 3D environment. In this study, we applied morphological and cytotoxicity evaluations, as well as microarray-based gene expression to examine the effects of different phthalate esters (PE) on this model. Known in vivo male developmentally toxic PEs (DTPE) and developmentally non-toxic PEs (DNTPE) were evaluated. We observed that DTPE induced significantly greater dose-dependent morphological changes, a decrease in cell viability and an increase in cytotoxicity compared to those treated with DNTPE. Moreover, the gene expression was more greatly altered by DTPE than by DNTPE and non-supervised cluster analysis allowed the discrimination of DTPE from the DNTPE. Our systems-based GO-Quant analysis showed significant alterations in the gene pathways involved in cell cycle, phosphate transport and apoptosis regulation with DTPE but not with DNTPE treatment. Disruptions of steroidogenesis related-gene expression such as Star, Cyp19a1, Hsd17b8, and Nr4a3 were observed in the DTPE group, but not in the DNTPE group. In summary, our observation on cell viability, cytotoxicity, and microarray-based gene expression analysis induced by PEs demonstrate that our in vitro 3D-SGC system mimicked in vivo responses for PEs and suggests that the 3D-SGC system might be useful in identifying developmental reproductive toxicants.

  13. Sprague-Dawley rats display metabolism-mediated sex differences in the acute toxicity of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)

    SciTech Connect (OSTI)

    Fonsart, Julien [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France); Menet, Marie-Claude [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[Universite Paris Descartes, Faculte de Pharmacie, Plateau Spectrometrie de Masse (IFR 71), Service de Chimie Analytique, Paris F-75006 (France); Decleves, Xavier [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France); Galons, Herve [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[INSERM, U648, Paris F-75006 (France); Crete, Dominique; Debray, Marcel; Scherrmann, Jean-Michel [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France); Noble, Florence [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France)], E-mail: florence.noble@univ-paris5.fr

    2008-07-01

    The use of the amphetamine derivative 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) has been associated with unexplained deaths. Male humans and rodents are more sensitive to acute toxicity than are females, including a potentially lethal hyperthermia. MDMA is highly metabolized to five main metabolites, by the enzymes CYP1A2 and CYP2D. The major metabolite in rats, 3,4-methylenedioxyamphetamine (MDA), also causes hyperthermia. We postulated that the reported sex difference in rats is due to a sexual dimorphism(s). We therefore determined (1) the LD50 of MDMA and MDA, (2) their hyperthermic effects, (3) the activities of liver CYP1A2 and CYP2D, (4) the liver microsomal metabolism of MDMA and MDA, (5) and the plasma concentrations of MDMA and its metabolites 3 h after giving male and female Sprague-Dawley (SD) rats MDMA (5 mg.kg{sup -1} sc). The LD50 of MDMA was 2.4-times lower in males than in females. MDMA induced greater hyperthermia (0.9 deg. C) in males. The plasma MDA concentration was 1.3-fold higher in males, as were CYP1A2 activity (twice) and N-demethylation to MDA (3.3-fold), but the plasma MDMA concentration (1.4-fold) and CYP2D activity (1.3-fold) were higher in females. These results suggest that male SD rats are more sensitive to MDMA acute toxicity than are females, probably because their CYP1A2 is more active, leading to higher N-demethylation and plasma MDA concentration. This metabolic pathway could be responsible for the lethality of MDMA, as the LD50 of MDA is the same in both sexes. These data strongly suggest that the toxicity of amphetamine-related drugs largely depends on metabolic differences.

  14. Local Control, Toxicity, and Cosmesis in Women >70 Years Enrolled in the American Society of Breast Surgeons Accelerated Partial Breast Irradiation Registry Trial

    SciTech Connect (OSTI)

    Khan, Atif J.; Vicini, Frank A.; Beitsch, Peter; Goyal, Sharad; Kuerer, Henry M.; Keisch, Martin; Quiet, Coral; Zannis, Victor; Keleher, Angela; Snyder, Howard; Gittleman, Mark; Whitworth, Pat; Fine, Richard; Lyden, Maureen; Haffty, Bruce G.; American Society of Breast Surgeons, Columbia, MD

    2012-10-01

    Purpose: The American Society of Breast Surgeons enrolled women in a registry trial to prospectively study patients treated with the MammoSite Radiation Therapy System breast brachytherapy device. The present report examined the outcomes in women aged >70 years enrolled in the trial. Methods and Materials: A total of 1,449 primary early stage breast cancers were treated in 1,440 women. Of these, 537 occurred in women >70 years old. Fisher's exact test was performed to correlate age ({<=}70 vs. >70 years) with toxicity and with cosmesis. The association of age with local recurrence (LR) failure times was investigated by fitting a parametric model. Results: Older women were less likely to develop telangiectasias than younger women (7.9% vs. 12.4%, p = 0.0083). The incidence of other toxicities was similar. Cosmesis was good or excellent in 92% of the women >70 years old. No significant difference was found in LR as a function of age. The 5-year actuarial LR rate with invasive disease for the older vs. younger population was 2.79% and 2.92%, respectively (p = 0.5780). In women >70 years with hormone-sensitive tumors {<=}2 cm who received hormonal therapy (n = 195), the 5-year actuarial rate of LR, overall survival, disease-free survival, and cause-specific survival was 2.06%, 89.3%, 87%, and 97.5%, respectively. These outcomes were similar in women who did not receive hormonal therapy. Women with small, estrogen receptor-negative disease had worse LR, overall survival, and disease-free survival compared with receptor-positive patients. Conclusions: Accelerated partial breast irradiation with the MammoSite radiation therapy system resulted in low toxicity and produced similar cosmesis and local control at 5 years in women >70 years compared with younger women. This treatment should be considered as an alternative to omitting adjuvant radiotherapy for older women with small-volume, early-stage breast cancer.

  15. Hypoxia inducible factor-1 (HIF-1)flavin containing monooxygenase-2 (FMO-2) signaling acts in silver nanoparticles and silver ion toxicity in the nematode, Caenorhabditis elegans

    SciTech Connect (OSTI)

    Eom, Hyun-Jeong; Ahn, Jeong-Min; Kim, Younghun; Choi, Jinhee

    2013-07-15

    In the present study, nanotoxicity mechanism associated with silver nanoparticles (AgNPs) exposure was investigated on the nematode, Caenorhabditis elegans focusing on the hypoxia response pathway. In order to test whether AgNPs-induced hypoxia inducible factor-1 (HIF-1) activation was due to hypoxia or to oxidative stress, depletion of dissolved oxygen (DO) in the test media and a rescue effect using an antioxidant were investigated, respectively. The results suggested that oxidative stress was involved in activation of the HIF-1 pathway. We then investigated the toxicological implications of HIF-1 activation by examining the HIF-1 mediated transcriptional response. Of the genes tested, increased expression of the flavin containing monooxygenase-2 (FMO-2) gene was found to be the most significant as induced by AgNPs exposure. We found that AgNPs exposure induced FMO-2 activation in a HIF-1 and p38 MAPK PMK-1 dependent manner, and oxidative stress was involved in it. We conducted all experiments to include comparison of AgNPs and AgNO{sub 3} in order to evaluate whether any observed toxicity was due to dissolution or particle specific. The AgNPs and AgNO{sub 3} did not produce any qualitative differences in terms of exerting toxicity in the pathways observed in this study, however, considering equal amount of silver mass, in every endpoint tested the AgNPs were found to be more toxic than AgNO{sub 3}. These results suggest that Ag nanotoxicity is dependent not only on dissolution of Ag ion but also on particle specific effects and HIF-1FMO-2 pathway seems to be involved in it. - Highlights: HIF-1 signaling was investigated in C. elegans exposed to AgNPs and AgNO{sub 3}. HIF-1 and PMK-1 were needed for AgNPs- and AgNO{sub 3}-induced fmo-2 gene expression. PMK-1HIF-1FMO-2 pathway was dependent on oxidative stress. AgNPs and AgNO{sub 3} did not produce any qualitative differences in HIF-1 signaling. AgNPs were more toxic than an equal amount of silver mass contained in AgNO{sub 3}.

  16. Long-term Survival and Toxicity in Patients Treated With High-Dose Intensity Modulated Radiation Therapy for Localized Prostate Cancer

    SciTech Connect (OSTI)

    Spratt, Daniel E.; Pei, Xin; Yamada, Josh; Kollmeier, Marisa A.; Cox, Brett; Zelefsky, Michael J.

    2013-03-01

    Purpose: To report long-term survival and toxicity outcomes with the use of high-dose intensity modulated radiation therapy (IMRT) to 86.4 Gy for patients with localized prostate cancer. Methods and Materials: Between August 1997 and December 2008, 1002 patients were treated to a dose of 86.4 Gy using a 5-7 field IMRT technique. Patients were stratified by prognostic risk group based on National Comprehensive Cancer Network risk classification criteria. A total of 587 patients (59%) were treated with neoadjuvant and concurrent androgen deprivation therapy. The median follow-up for the entire cohort was 5.5 years (range, 1-14 years). Results: For low-, intermediate-, and high-risk groups, 7-year biochemical relapse-free survival outcomes were 98.8%, 85.6%, and 67.9%, respectively (P<.001), and distant metastasis-free survival rates were 99.4%, 94.1%, and 82.0% (P<.001), respectively. On multivariate analysis, T stage (P<.001), Gleason score (P<.001), and >50% of initial biopsy positive core (P=.001) were predictive for distant mestastases. No prostate cancer-related deaths were observed in the low-risk group. The 7-year prostate cancer-specific mortality (PCSM) rates, using competing risk analysis for intermediate- and high-risk groups, were 3.3% and 8.1%, respectively (P=.008). On multivariate analysis, Gleason score (P=.004), percentage of biopsy core positivity (P=.003), and T-stage (P=.033) were predictive for PCSM. Actuarial 7-year grade 2 or higher late gastrointestinal and genitourinary toxicities were 4.4% and 21.1%, respectively. Late grade 3 gastrointestinal and genitourinary toxicity was experienced by 7 patients (0.7%) and 22 patients (2.2%), respectively. Of the 427 men with full potency at baseline, 317 men (74%) retained sexual function at time of last follow-up. Conclusions: This study represents the largest cohort of patients treated with high-dose radiation to 86.4 Gy, using IMRT for localized prostate cancer, with the longest follow-up to date. Our findings indicate that this treatment results in excellent clinical outcomes with acceptable toxicity.

  17. Five-Year Outcomes, Cosmesis, and Toxicity With 3-Dimensional Conformal External Beam Radiation Therapy to Deliver Accelerated Partial Breast Irradiation

    SciTech Connect (OSTI)

    Rodrguez, Nria; Sanz, Xavier; Dengra, Josefa; Foro, Palmira; Membrive, Ismael; Reig, Anna; Quera, Jaume; Fernndez-Velilla, Enric; Pera, scar; Lio, Jackson; Lozano, Joan; Algara, Manuel

    2013-12-01

    Purpose: To report the interim results from a study comparing the efficacy, toxicity, and cosmesis of breast-conserving treatment with accelerated partial breast irradiation (APBI) or whole breast irradiation (WBI) using 3-dimensional conformal external beam radiation therapy (3D-CRT). Methods and Materials: 102 patients with early-stage breast cancer who underwent breast-conserving surgery were randomized to receive either WBI (n=51) or APBI (n=51). In the WBI arm, 48 Gy was delivered to the whole breast in daily fractions of 2 Gy, with or without additional 10 Gy to the tumor bed. In the APBI arm, patients received 37.5 Gy in 3.75 Gy per fraction delivered twice daily. Toxicity results were scored according to the Radiation Therapy Oncology Group Common Toxicity Criteria. Skin elasticity was measured using a dedicated device (Multi-Skin-Test-Center MC-750-B2, CKelectronic-GmbH). Cosmetic results were assessed by the physician and the patients as good/excellent, regular, or poor. Results: The median follow-up time was 5 years. No local recurrences were observed. No significant differences in survival rates were found. APBI reduced acute side effects and radiation doses to healthy tissues compared with WBI (P<.01). Late skin toxicity was no worse than grade 2 in either group, without significant differences between the 2 groups. In the ipsilateral breast, the areas that received the highest doses (ie, the boost or quadrant) showed the greatest loss of elasticity. WBI resulted in a greater loss of elasticity in the high-dose area compared with APBI (P<.05). Physician assessment showed that >75% of patients in the APBI arm had excellent or good cosmesis, and these outcomes appear to be stable over time. The percentage of patients with excellent/good cosmetic results was similar in both groups. Conclusions: APBI delivered by 3D-CRT to the tumor bed for a selected group of early-stage breast cancer patients produces 5-year results similar to those achieved with conventional WBI.

  18. foal

    National Nuclear Security Administration (NNSA)

    and dispose of many different hazardous substances, including radioactive materials, toxic chemicals, and biological agents and toxins.

    There are a few programs NNSA uses...

  19. Federal Facility Agreement for the Laboratory for Energy-Related...

    Office of Environmental Management (EM)

    appropriate response actions at LEHR Parties DOE; USEPA; California Department of Toxic Substances Control; Central Valley Regional Water Quality Control Board; California...

  20. General Atomics Compliance Order, October 6, 1995 Summary

    Office of Environmental Management (EM)

    Atomics Parties DOE; State of California Environmental Protection Agency (Department of Toxic Substances Control) Date 1061995 SCOPE * Address LDR requirements pertaining to...

  1. Job Safety

    Broader source: Energy.gov (indexed) [DOE]

    health poster; Copies of their medical records and records of their exposures to toxic and harmful substances or conditions; and Results of inspections and accident...

  2. Energy Technology and Engineering Center Compliance Order, October...

    Office of Environmental Management (EM)

    at ETEC Parties DOE; State of California Environmental Protection Agency (Department of Toxic Substances Control) Date 1061995 SCOPE * Address LDR requirements pertaining to...

  3. emt

    National Nuclear Security Administration (NNSA)

    and dispose of many different hazardous substances, including radioactive materials, toxic chemicals, and biological agents and toxins.

    There are a few programs NNSA uses...

  4. exercise program

    National Nuclear Security Administration (NNSA)

    and dispose of many different hazardous substances, including radioactive materials, toxic chemicals, and biological agents and toxins.

    There are a few programs NNSA uses...

  5. file://L:\\DOE-hanford.gov\\public\\boards\\hab\\advice\\advice27.htm

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    substances to meet the residential use cleanup standards of the Washington Model Toxics Control Act. There is no apparent residual radioactive contamination above background...

  6. Permit by Rule Guide | Open Energy Information

    Open Energy Info (EERE)

    Permit by Rule GuideLegal Abstract California Department of Toxic Substance Control Permit by Rule Guide, current through August 7, 2014. Published NA Year Signed or...

  7. A modified inflammatory bowel disease questionnaire and the Vaizey Incontinence questionnaire are more sensitive measures of acute gastrointestinal toxicity during pelvic radiotherapy than RTOG grading

    SciTech Connect (OSTI)

    Khalid, Usman; McGough, Camilla; Hackett, Claire; Blake, Peter; Harrington, Kevin J.; Khoo, Vincent S.; Tait, Diana; Norman, Andrew R.; Andreyev, H. Jervoise N. . E-mail: j@andreyev.demon.co.uk

    2006-04-01

    Purpose: Simple scales with greater sensitivity than Radiation Therapy Oncology Group (RTOG) grading to detect acute gastrointestinal toxicity during pelvic radiotherapy, could be clinically useful. Methods and Materials: Do questionnaires used in benign gastrointestinal diseases detect toxicity in patients undergoing radiotherapy? The patient-completed Inflammatory Bowel Disease (IBDQ) and Vaizey Incontinence questionnaires were compared prospectively at baseline and at Week 5 to physician-completed RTOG grading. Results: A total of 107 patients, median age 63 years, were recruited. After 5 weeks of treatment, patients with gynecologic and gastrointestinal cancer were more symptomatic than urologic patients (p 0.012; p = 0.014). Overall, 94% had altered bowel habits, 80% loose stool, 74% frequency, 65% difficult gas, 60% pain, >48% distress, 44% tenesmus, >40% restrictions in daily activity, 39% urgency, 37% fecal incontinence, and 40% required antidiarrheal medication. The median RTOG score was 1 (range, 0-2), median IBDQ score 204.5 (range, 74-224), and median Vaizey score 5 (range, 0-20). Chemotherapy preceding radiotherapy increased fecal incontinence (p 0.002). RTOG scores stabilized after 3 weeks, IBDQ scores peaked at Week 4, and Vaizey scores worsened throughout treatment. IBDQ and Vaizey scores distinguished between groups with different RTOG scores. Conclusion: The IBDQ and Vaizey questionnaires are reliable and sensitive, offering greater insight into the severity and range of symptoms compared with RTOG grading.

  8. Environmental assessment for the construction and operation of waste storage facilities at the Paducah Gaseous Diffusion Plant, Paducah, Kentucky

    SciTech Connect (OSTI)

    1994-06-01

    DOE is proposing to construct and operate 3 waste storage facilities (one 42,000 ft{sup 2} waste storage facility for RCRA waste, one 42,000 ft{sup 2} waste storage facility for toxic waste (TSCA), and one 200,000 ft{sup 2} mixed (hazardous/radioactive) waste storage facility) at Paducah. This environmental assessment compares impacts of this proposed action with those of continuing present practices aof of using alternative locations. It is found that the construction, operation, and ultimate closure of the proposed waste storage facilities would not significantly affect the quality of the human environment within the meaning of NEPA; therefore an environmental impact statement is not required.

  9. Sensitivity to methylmercury toxicity is enhanced in oxoguanine glycosylase 1 knockout murine embryonic fibroblasts and is dependent on cellular proliferation capacity

    SciTech Connect (OSTI)

    Ondovcik, Stephanie L.; Tamblyn, Laura; McPherson, John Peter; Wells, Peter G.

    2013-07-01

    Methylmercury (MeHg) is a persistent environmental contaminant with potent neurotoxic action for which the underlying molecular mechanisms remain to be conclusively delineated. Our objectives herein were twofold: first, to corroborate our previous findings of an increased sensitivity of spontaneously-immortalized oxoguanine glycosylase 1-null (Ogg1{sup ?/?}) murine embryonic fibroblasts (MEFs) to MeHg through generation of Simian virus 40 (SV40) large T antigen-immortalized wild-type and Ogg1{sup ?/?} MEFs; and second, to determine whether MeHg toxicity is proliferation-dependent. As with the spontaneously-immortalized cells used previously, the SV40 large T antigen-immortalized cells exhibited similar tendencies to undergo MeHg-initiated cell cycle arrest, with increased sensitivity in the Ogg1{sup ?/?} MEFs as measured by clonogenic survival and DNA damage. Compared to exponentially growing cells, those seeded at a higher density exhibited compromised proliferation, which proved protective against MeHg-mediated cell cycle arrest and induction of DNA double strand breaks (DSBs), measured by phosphorylation of the core histone H2A variant (H2AX) on serine 139 (?H2AX), and by its functional confirmation by micronucleus assessment. This enhanced sensitivity of Ogg1{sup ?/?} MEFs to MeHg toxicity using discrete SV40 immortalization corroborates our previous studies, and suggests a novel role for OGG1 in minimizing MeHg-initiated DNA lesions that trigger replication-associated DSBs. Furthermore, proliferative capacity may determine MeHg toxicity in vivo and in utero. Accordingly, variations in cellular proliferative capacity and interindividual variability in repair activity may modulate the risk of toxicological consequences following MeHg exposure. - Highlights: SV40 large T antigen-immortalized Ogg1{sup ?/?} cells are more sensitive to MeHg. Sensitivity to MeHg is dependent on cellular proliferation capacity. OGG1 maintains genomic integrity following MeHg-initiated DNA damage. OGG1 may limit MeHg-initiated DNA lesions that trigger replication-associated DSBs. Variations in proliferation and repair activity may modulate toxicological risk.

  10. The role of intrahepatic CD3 +/CD4 −/CD8 − double negative T (DN T) cells in enhanced acetaminophen toxicity

    SciTech Connect (OSTI)

    Getachew, Yonas; Cusimano, Frank A.; James, Laura P.; Thiele, Dwain L.

    2014-10-15

    The role of the immune system, specifically NK, NKT and CD3 cells, in acetaminophen (APAP) induced liver injury remains inconsistently defined. In the present study, wild type (C57BL/6J) mice and granzyme B deficient (GrB −/−) mice were treated with acetaminophen to assess the role of the immune system in acute liver injury. Doses of acetaminophen that induced sub lethal liver injury in wild type mice unexpectedly produced fatal hepatotoxicity in granzyme B deficient (GrB −/−) mice. Analysis revealed that GrB −/− mice had an increased population of intrahepatic CD3 (+), CD4 (−), and CD8 (−) lymphocytes expressing the CD69 activation marker and Fas ligand. Depletion of these cells in the GrB −/− and wild type mice made them less susceptible to APAP injury, while depletion of NK1.1 (+) cells or both CD4 (+) and CD8 (+) T cells failed to provide the same hepatoprotection. Transfer of the GrB −/− IHLs further exacerbated liver injury and increased mortality in wild type mice but not in LRP/LPR mice, lacking fas expression. Conclusions: Acetaminophen toxicity is enhanced by the presence of activated, FasL expressing intrahepatic CD3 (+), CD4 (−), CD8 (−), NK1.1 (−) T cells. Depletion of these cells from GrB −/− mice and wild type mice greatly reduces mortality and improves the course of liver injury recovery. - Highlights: • Intrahepatic lymphocytes (IHLs) from GrB −/− mice harbor activated DNT cells. • IHLs from GrB −/− mice exhibit enhanced Fas ligand expression. • Acetaminophen toxicity is enhanced by activated, FasL expressing DNT cells.

  11. Late Patient-Reported Toxicity After Preoperative Radiotherapy or Chemoradiotherapy in Nonresectable Rectal Cancer: Results From a Randomized Phase III Study

    SciTech Connect (OSTI)

    Braendengen, Morten, E-mail: mortbrae@medisin.uio.no [Oslo University Hospital, Ulleval, Cancer Centre, Oslo (Norway); Department of Oncology and Pathology, Karolinska Institutet, Stockholm (Sweden); Tveit, Kjell Magne [Oslo University Hospital, Ulleval, Cancer Centre, Oslo (Norway); Faculty of Medicine, University of Oslo, Oslo (Norway); Bruheim, Kjersti [Oslo University Hospital, Ulleval, Cancer Centre, Oslo (Norway); Cvancarova, Milada [Department of Clinical Cancer Research, Oslo University Hospital, Radiumhospitalet, Oslo (Norway); Berglund, Ake [Department of Oncology, Radiology and Clinical Immunology, University of Uppsala, Uppsala (Sweden); Glimelius, Bengt [Department of Oncology and Pathology, Karolinska Institutet, Stockholm (Sweden); Department of Oncology, Radiology and Clinical Immunology, University of Uppsala, Uppsala (Sweden)

    2011-11-15

    Purpose: Preoperative chemoradiotherapy (CRT) is superior to radiotherapy (RT) in locally advanced rectal cancer, but the survival gain is limited. Late toxicity is, therefore, important. The aim was to compare late bowel, urinary, and sexual functions after CRT or RT. Methods and Materials: Patients (N = 207) with nonresectable rectal cancer were randomized to preoperative CRT or RT (2 Gy Multiplication-Sign 25 {+-} 5-fluorouracil/leucovorin). Extended surgery was often required. Self-reported late toxicity was scored according to the LENT SOMA criteria in a structured telephone interview and with questionnaires European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire (QLQ-C30), International Index of Erectile Function (IIEF), and sexual function -vaginal changes questionnaire (SVQ). Results: Of the 105 patients alive in Norway and Sweden after 4 to 12 years of follow-up, 78 (74%) responded. More patients in the CRT group had received a stoma (73% vs. 52%, p = 0.09). Most patients without a stoma (7 of 12 in CRT group and 9 of 16 in RT group) had incontinence for liquid stools or gas. No stoma and good anal function were seen in 5 patients (11%) in the CRT group and in 11 (30%) in the RT group (p = 0.046). Of 44 patients in the CRT group, 12 (28%) had had bowel obstruction compared with 5 of 33 (15%) in the RT group (p = 0.27). One-quarter of the patients reported urinary incontinence. The majority of men had severe erectile dysfunction. Few women reported sexual activity during the previous month. However, the majority did not have concerns about their sex life. Conclusions: Fecal incontinence and erectile dysfunction are frequent after combined treatment for locally advanced rectal cancer. There was a clear tendency for the problems to be more common after CRT than after RT.

  12. Androgenic endocrine disruptors in wastewater treatment plant effluents in India: Their influence on reproductive processes and systemic toxicity in male rats

    SciTech Connect (OSTI)

    Kumar, Vikas; Chakraborty, Ajanta; Viswanath, Gunda; Roy, Partha

    2008-01-01

    Endocrine-disrupting chemicals (EDC) are linked to human health and diseases as they mimic or block the normal functioning of endogenous hormones. The present work dealt with a comparative study of the androgenic potential of wastewater treatment plant (WWTP) influents and effluents in Northern region of India, well known for its polluted water. Water samples were screened for their androgenic potential using the Hershberger assay and when they were found positive for androgenicity, we studied their mode of action in intact rats. The data showed a significant change in the weight and structure of sex accessory tissues (SATs) of castrated and intact rats. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis demonstrated a significant change in the expression patterns of the major steroidogenic enzymes in adrenal and testis: cytochrome P450{sub SCC}, cytochrome P450{sub C17}, 3{beta}-hydroxysteroid dehydrogenase, 17{beta}-hydroxysteroid dehydrogenase. This was further supported by increased enzymatic activities measured in vitro spectrophotometrically. Serum hormone profile showed a decreased level of gonadotrophic hormones and increased testosterone level. Further, increase in the serum level of alkaline phosphatase, SGPT and SGOT and histopathological changes in kidney and liver of treated animals, confirmed the toxic effects of contaminating chemicals. Analysis of water samples using HPLC and GC-MS showed the presence of various compounds and from them, four prominent aromatic compounds viz. nonylphenol, hexachlorobenzene and two testosterone equivalents, were identified. Our data suggest that despite rigorous treatment, the final treated effluent from WWTP still has enough androgenic and toxic compounds to affect general health.

  13. Toxicity studies on Agents GB and GD (Phase 2): 90-day subchronic study of GB (Sarin, Type I) in CD rats. Final report, Jul 85-Aug 91

    SciTech Connect (OSTI)

    Bucci, T.J.; Parker, R.M.; Crowell, J.A.; Thurman, J.D.; Gosnell, P.A.

    1991-08-01

    A two-phase Dose Range finding study and a 90-Day Subchronic study were conducted in CD rats using the organophosphate ester Sarin (Agent GB, Type I, CAS Number 107-44-8). The highest dose level without lethality in the second phase of the range finding study was designated the maximum tolerated dose (MTD). The doses selected for the subchronic study were the MTD (300 micron GBI/Kg/day), MTD/2 (150, micron GBI/Kg/day), MTD/4 (75 micron GBI/Kg/day), and a vehicle control (O micron /Kg/day). Forty-eight male and forty-eight female CD rats were randomly allocated at 11-12 weeks of age into four treatment groups (12 per sex per group). The animals were gavaged Monday through Friday for 13 weeks and euthanized with carbon dioxide at the beginning of the fourteenth week. Animals were observed daily for clinical signs of toxicity and were weighed weekly. The rats were bled (6 rats/sex/dose) during weeks -1, 1, 3, 7, and at necropsy. Necropsy examination was performed on all animals. Microscopic evaluation was performed on all high-dose and control animals, and on those tissues of lower dose animals that were abnormal at necropsy. All gross lesions and all animals dying or removed early received histological examination. A cause of death or morbidity for animals removed before the end of the study, determined from histopathological examination, was established in four of the eight cases. There were several statistically significant effects in the clinical chemistry and hematology data. These effects were scattered among the treatment groups and were not numerous enough to develop a pattern of organ toxicity.

  14. Evaluations in support of regulatory and research decisions by the U. S. Environmental Protection Agency for the control of toxic hazards from hazardous wastes, glyphosate, dalapon, and synthetic fuels

    SciTech Connect (OSTI)

    Scofield, R.

    1984-01-01

    This report includes toxicological and regulatory evaluations performed in support of U.S. EPA regulation of toxic materials and hazardous wastes. The first section of the report describes evaluations which support: (a) the regulation of small-volume generators of hazardous wastes, (b) the regulation of hazardous wastes from pesticide manufacturing, and (c) the disposal of the herbicide, silvex. The second section describes the environmental fate, transport, and effect of glyphosate and dalapon. The third section deals with synthetic fuels, including evaluations of synfuel-product toxicity, uncontrolled air emissions, and particular focus on the toxicity of products from several indirect coal liquefaction processes including methanol synthesis, Fischer-Tropsch, Mobil M-Gasoline, and Lurgi gasification technologies. Three direct coal liquefaction processes were examined for product toxicity and air emissions: Solvent Refined Coal (I and II) and the Exxon Donor Solvent Process. Also described in the third section is an evaluation of environmental and health hazards associated with the use of synthetic fuels from indirect coal liquefaction, direct coal liquefaction, and shale oil. Finally, the fourth section discusses some problems associated with performing, on a contractual basis, scientific and technical evaluations in support of U.S. EPA regulatory and research decisions.

  15. One-of-a-Kind Facility Now in Safe Shutdown | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The TSCA incinerator began operations in 1991, when it was the only facility in the nation capable of incinerating hazardous and radioactive wastes containing PCBs. The facility's ...

  16. Extended (5-year) Outcomes of Accelerated Partial Breast Irradiation Using MammoSite Balloon Brachytherapy: Patterns of Failure, Patient Selection, and Dosimetric Correlates for Late Toxicity

    SciTech Connect (OSTI)

    Vargo, John A.; Verma, Vivek; Kim, Hayeon; Kalash, Ronny; Heron, Dwight E.; Johnson, Ronald; Beriwal, Sushil

    2014-02-01

    Purpose: Accelerated partial breast irradiation (APBI) with balloon and catheter-based brachytherapy has gained increasing popularity in recent years and is the subject of ongoing phase III trials. Initial data suggest promising local control and cosmetic results in appropriately selected patients. Long-term data continue to evolve but are limited outside of the context of the American Society of Breast Surgeons Registry Trial. Methods and Materials: A retrospective review of 157 patients completing APBI after breast-conserving surgery and axillary staging via high-dose-rate {sup 192}Ir brachytherapy from June 2002 to December 2007 was made. APBI was delivered with a single-lumen MammoSite balloon-based applicator to a median dose of 34 Gy in 10 fractions over a 5-day period. Tumor coverage and critical organ dosimetry were retrospectively collected on the basis of computed tomography completed for conformance and symmetry. Results: At a median follow-up time of 5.5 years (range, 0-10.0 years), the 5-year and 7-year actuarial incidences of ipsilateral breast control were 98%/98%, of nodal control 99%/98%, and of distant control 99%/99%, respectively. The crude rate of ipsilateral breast recurrence was 2.5% (n=4); of nodal failure, 1.9% (n=3); and of distant failure, 0.6% (n=1). The 5-year and 7-year actuarial overall survival rates were 89%/86%, with breast cancerspecific survival of 100%/99%, respectively. Good to excellent cosmetic outcomes were achieved in 93.4% of patients. Telangiectasia developed in 27% of patients, with 1-year, 3-year, and 5-year actuarial incidence of 7%/24%/33%; skin dose >100% significantly predicted for the development of telangiectasia (50% vs 14%, P<.0001). Conclusions: Long-term single-institution outcomes suggest excellent tumor control, breast cosmesis, and minimal late toxicity. Skin toxicity is a function of skin dose, which may be ameliorated with dosimetric optimization afforded by newer multicatheter brachytherapy applicators and a more rigorous skin dose constraint of ?100%.

  17. Final report on the developmental toxicity of ethylenediamine (CAS No. 107-15-3) in New Zealand white rabbits. Report for November-February 1992

    SciTech Connect (OSTI)

    Not Available

    1993-03-01

    Ethylenediamine (EDA) is a major industrial chemical with an estimated U.S. production of 64 million pounds in 1985. EDA is used as a chemical intermediate or solvent in manufacturing, as a paint thinner, and as a constituent of certain cosmetic, pharmaceutical and veterinary products. Based upon its widespread applications and the potential for exposure in pregnant women, EDA was evaluated for maternal and developmental toxicity using a laboratory animal model. Artificially-inseminated New Zealand White rabbits (26/group) were administered ethylenediamine (0, 10, 40 or 80 mg/kg/day) by gavage on gestational days (gd) 6 through 19. The doses administered were equivalent to 0, 22, 89 or 178 of EDA.2HC1. Maternal clinical signs, body weight, and food consumption were monitored at regular intervals throughout gestation. At termination (gd 30), the uterus was removed and examined to determine pregnancy status and to evaluate the number of resorptions, and dead or live fetuses. Dead or live fetuses were weighed, and live fetuses examined for external, visceral and skeletal defects.

  18. A CHRONIC INHALATION STUDY OF METHYL BROMIDE TOXICITY IN B6C3F1 MICE. (FINAL REPORT TO THE NATIONAL TOXICOLOGY PROGRAM)

    SciTech Connect (OSTI)

    HABER, S.B.

    1987-06-26

    This report provides a detailed account of a two year chronic inhalation study of methyl bromide toxicity in B6C3Fl mice conducted for the National Toxicology Program. Mice were randomized into three dose groups (10, 33 and 100 ppm methyl bromide) and one control group (0 ppm) per sex and exposed 5 days/week, 6 hours/day, for a total of 103 weeks. Endpoints included body weight; clinical signs and mortality, and at 6, 15 and 24 months of exposure, animals were sacrificed for organ weights, hematology and histopathology. In addition, a subgroup of animals in each dosage group was monitored for neurobehavioral and neuropathological changes. After only 20 weeks of exposure, 48% of the males and 12% of the females in the 100 ppm group had died. Exposures were terminated in that group and the surviving mice were observed for the duration of the study. Exposure of B6C3Fl mice to methyl bromide, even for only 20 weeks, produced significant changes in growth rate, mortality, organ weights and neurobehavioral functioning. These changes occurred in both males and females, but were more pronounced in males.

  19. Characterizing toxic emissions from a coal-fired power plant demonstrating the AFGD ICCT Project and a plant utilizing a dry scrubber/baghouse system: Bailly Station Units 7 and 8 and AFGD ICCT Project. Final report. Final report

    SciTech Connect (OSTI)

    Dismukes, E.B.

    1994-10-20

    This report describes results of assessment of the risk of emissions of hazardous air pollutants at one of the electric power stations, Bailly Station, which is also the site of a Clean Coal Technology project demonstrating the Pure Air Advanced Flue Gas Desulfurization process (wet limestone). This station represents the configuration of no NO{sub x} reduction, particulate control with electrostatic precipitators, and SO{sub 2} control with a wet scrubber. The test was conducted September 3--6, 1993. Sixteen trace metals were determined along with 5 major metals. Other inorganic substances and organic compounds were also determined.

  20. Application for a Permit to Operate a Class III Solid Waste Disposal Site at the Nevada Test Site Area 5 Asbestiform Low-Level Solid Waste Disposal Site

    SciTech Connect (OSTI)

    NSTec Environmental Programs

    2010-09-14

    The NTS solid waste disposal sites must be permitted by the state of Nevada Solid Waste Management Authority (SWMA). The SWMA for the NTS is the Nevada Division of Environmental Protection, Bureau of Federal Facilities (NDEP/BFF). The U.S. Department of Energy's National Nuclear Security Administration Nevada Site Office (NNSA/NSO) as land manager (owner), and National Security Technologies (NSTec), as operator, will store, collect, process, and dispose all solid waste by means that do not create a health hazard, a public nuisance, or cause impairment of the environment. NTS disposal sites will not be included in the Nye County Solid Waste Management Plan. The NTS is located approximately 105 kilometers (km) (65 miles [mi]) northwest of Las Vegas, Nevada (Figure 1). The U.S. Department of Energy (DOE) is the federal lands management authority for the NTS, and NSTec is the Management and Operations contractor. Access on and off the NTS is tightly controlled, restricted, and guarded on a 24-hour basis. The NTS has signs posted along its entire perimeter. NSTec is the operator of all solid waste disposal sites on the NTS. The Area 5 RWMS is the location of the permitted facility for the Solid Waste Disposal Site (SWDS). The Area 5 RWMS is located near the eastern edge of the NTS (Figure 2), approximately 26 km (16 mi) north of Mercury, Nevada. The Area 5 RWMS is used for the disposal of low-level waste (LLW) and mixed low-level waste. Many areas surrounding the RWMS have been used in conducting nuclear tests. A Notice of Intent to operate the disposal site as a Class III site was submitted to the state of Nevada on January 28, 1994, and was acknowledged as being received in a letter to the NNSA/NSO on August 30, 1994. Interim approval to operate a Class III SWDS for regulated asbestiform low-level waste (ALLW) was authorized on August 12, 1996 (in letter from Paul Liebendorfer to Runore Wycoff), with operations to be conducted in accordance with the ''Management Plan for the Disposal of Low-Level Waste with Regulated Asbestos Waste.'' A requirement of the authorization was that on or before October 9, 1999, a permit was required to be issued. Because of NDEP and NNSA/NSO review cycles, the final permit was issued on April 5, 2000, for the operation of the Area 5 Low-Level Waste Disposal Site, utilizing Pit 7 (P07) as the designated disposal cell. The original permit applied only to Pit 7, with a total design capacity of 5,831 cubic yards (yd{sup 3}) (157,437 cubic feet [ft{sup 3}]). NNSA/NSO is expanding the SWDS to include the adjacent Upper Cell of Pit 6 (P06), with an additional capacity of 28,037 yd{sup 3} (756,999 ft{sup 3}) (Figure 3). The proposed total capacity of ALLW in Pit 7 and P06 will be approximately 33,870 yd{sup 3} (0.9 million ft{sup 3}). The site will be used for the disposal of regulated ALLW, small quantities of low-level radioactive hydrocarbon-burdened (LLHB) media and debris, LLW, LLW that contains PCB Bulk Product Waste greater than 50 ppm that leaches at a rate of less than 10 micrograms of PCB per liter of water, and small quantities of LLHB demolition and construction waste (hereafter called permissible waste). Waste containing free liquids, or waste that is regulated as hazardous waste under the Resource Conservation and Recovery Act (RCRA) or state-of-generation hazardous waste regulations, will not be accepted for disposal at the site. The only waste regulated under the Toxic Substances Control Act (TSCA) that will be accepted at the disposal site is regulated asbestos-containing materials (RACM). The term asbestiform is used throughout this document to describe this waste. Other TSCA waste (i.e., polychlorinated biphenyls [PCBs]) will not be accepted for disposal at the SWDS. The disposal site will be used as a depository of permissible waste generated both on site and off site. All generators designated by NNSA/NSO will be eligible to dispose regulated ALLW at the Asbestiform Low-Level Waste Disposal Site in accordance with the U.S. Department of Energy, Nevada Operations Office (DOE/NV) 325

  1. Authorized limits for disposal of PCB capacitors from Buildings 361 and 391 at Argonne National Laboratory, Argonne, Illinois.

    SciTech Connect (OSTI)

    Cheng, J.-J.; Chen, S.-Y.; Environmental Science Division

    2009-12-22

    This report contains data and analyses to support the approval of authorized release limits for the clearance from radiological control of polychlorinated biphenyl (PCB) capacitors in Buildings 361 and 391 at Argonne National Laboratory, Argonne, Illinois. These capacitors contain PCB oil that must be treated and disposed of as hazardous waste under the Toxic Substances Control Act (TSCA). However, they had been located in radiological control areas where the potential for neutron activation existed; therefore, direct release of these capacitors to a commercial facility for PCB treatment and landfill disposal is not allowable unless authorized release has been approved. Radiological characterization found no loose contamination on the exterior surface of the PCB capacitors; gamma spectroscopy analysis also showed the radioactivity levels of the capacitors were either at or slightly above ambient background levels. As such, conservative assumptions were used to expedite the analyses conducted to evaluate the potential radiation exposures of workers and the general public resulting from authorized release of the capacitors; for example, the maximum averaged radioactivity levels measured for capacitors nearest to the beam lines were assumed for the entire batch of capacitors. This approach overestimated the total activity of individual radionuclide identified in radiological characterization by a factor ranging from 1.4 to 640. On the basis of this conservative assumption, the capacitors were assumed to be shipped from Argonne to the Clean Harbors facility, located in Deer Park, Texas, for incineration and disposal. The Clean Harbors facility is a state-permitted TSCA facility for treatment and disposal of hazardous materials. At this facility, the capacitors are to be shredded and incinerated with the resulting incineration residue buried in a nearby landfill owned by the company. A variety of receptors that have the potential of receiving radiation exposures were analyzed. Based on the dose assessment results, it is indicated that, if the disposition activities are completed within a year, the maximum individual dose would be about 0.021 mrem/yr, which is about 0.02% of the primary dose limit of 100 mrem/yr set by U.S. Department of Energy (DOE) for members of the public. The maximum individual dose was associated with a conservative and unlikely scenario involving a hypothetical farmer who intruded the landfill area to set up a subsistence living above the disposal area 30 years after burial of the incineration residue. Potential collective dose for worker and the general public combined was estimated to be less than 4 x 10{sup -4} person-rem/yr, about 0.004% of the DOE authorized release objective of 10 person-rem/yr for collective exposure. In reality, the actual radiation doses incurred by workers and the general public are expected to be at least two orders of magnitude lower than the estimated values. To follow the ALARA (as low as reasonably achievable) principle of reducing potential radiation exposures associated with authorized release of the PCB capacitors, a dose constraint of 1 mrem/yr, corresponding to a small fraction of the 25 mrem/yr limit set by DOE, was initially used as a reference to derive the authorized release limits. On the basis of the dose assessment results, the following authorized release limits are proposed - 0.6 pCi/g for Mn-54, 0.6 pCi/g for Na-22, 0.1 pCi/g for Co-57, and 2.3 pCi/g for Co-60, with a corresponding maximum individual dose of 0.21 mrem/yr. This maximum dose, about 0.2% of the DOE primary dose limit of 100 mrem/yr for members of the public from all sources and exposure pathways, was then selected as the final dose constraint for releasing the PCB capacitors through the authorized process. The proposed authorized release limits would satisfy the DOE requirements for the release of non-real properties to a commercial treatment and disposal facility. In addition, due to the relatively short half-lives (< 5.27 years) of radionuclides of concern, there will be no long-term buil

  2. Late Toxicity and Patient Self-Assessment of Breast Appearance/Satisfaction on RTOG 0319: A Phase 2 Trial of 3-Dimensional Conformal Radiation Therapy-Accelerated Partial Breast Irradiation Following Lumpectomy for Stages I and II Breast Cancer

    SciTech Connect (OSTI)

    Chafe, Susan; Moughan, Jennifer; McCormick, Beryl; Wong, John; Pass, Helen; Rabinovitch, Rachel; Arthur, Douglas W.; Petersen, Ivy; White, Julia; Vicini, Frank A.

    2013-08-01

    Purpose: Late toxicities and cosmetic analyses of patients treated with accelerated partial breast irradiation (APBI) on RTOG 0319 are presented. Methods and Materials: Patients with stages I to II breast cancer ?3 cm, negative margins, and ?3 positive nodes were eligible. Patients received three-dimensional conformal external beam radiation therapy (3D-CRT; 38.5 Gy in 10 fractions twice daily over 5 days). Toxicity and cosmesis were assessed by the patient (P), the radiation oncologist (RO), and the surgical oncologist (SO) at 3, 6, and 12 months from the completion of treatment and then annually. National Cancer Institute Common Terminology Criteria for Adverse Events, version 3.0, was used to grade toxicity. Results: Fifty-two patients were evaluable. Median follow-up was 5.3 years (range, 1.7-6.4 years). Eighty-two percent of patients rated their cosmesis as good/excellent at 1 year, with rates of 64% at 3 years. At 3 years, 31 patients were satisfied with the treatment, 5 were not satisfied but would choose 3D-CRT again, and none would choose standard radiation therapy. The worst adverse event (AE) per patient reported as definitely, probably, or possibly related to radiation therapy was 36.5% grade 1, 50% grade 2, and 5.8% grade 3 events. Grade 3 AEs were all skin or musculoskeletal-related. Treatment-related factors were evaluated to potentially establish an association with observed toxicity. Surgical bed volume, target volume, the number of beams used, and the use of bolus were not associated with late cosmesis. Conclusions: Most patients enrolled in RTOG 0319 were satisfied with their treatment, and all would choose to have the 3D-CRT APBI again.

  3. Ultrasonic Nakagami-parameter characterization of parotid-gland injury following head-and-neck radiotherapy: A feasibility study of late toxicity

    SciTech Connect (OSTI)

    Yang, Xiaofeng; Wu, Ning; Wang, Yuefeng; Tridandapani, Srini; School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332; Winship Cancer Institute, Emory University, Atlanta, Georgia 30322 ; Beitler, Jonathan J.; Yu, David S.; Curran, Walter J.; Liu, Tian; Bruner, Deborah W.; Winship Cancer Institute, Emory University, Atlanta, Georgia 30322; School of Nursing, Emory University, Atlanta, Georgia 30322

    2014-02-15

    Purpose: The study aims to investigate whether Nakagami parametersestimated from the statistical distribution of the backscattered ultrasound radio-frequency (RF) signalscould provide a means for quantitative characterization of parotid-gland injury resulting from head-and-neck radiotherapy. Methods: A preliminary clinical study was conducted with 12 postradiotherapy patients and 12 healthy volunteers. Each participant underwent one ultrasound study in which ultrasound scans were performed in the longitudinal, i.e., vertical orientation on the bilateral parotids. For the 12 patients, the mean radiation dose to the parotid glands was 37.7 9.5 Gy, and the mean follow-up time was 16.3 4.8 months. All enrolled patients experienced grade 1 or 2 late salivary-gland toxicity (RTOG/EORTC morbidity scale). The normal parotid glands served as the control group. The Nakagami-scaling and Nakagami-shape parameters were computed from the RF data to quantify radiation-induced parotid-gland changes. Results: Significant differences in Nakagami parameters were observed between the normal and postradiotherapy parotid glands. Compared with the control group, the Nakagami-scaling parameter of the postradiotherapy group decreased by 25.8% (p < 0.001), and the Nakagami-shape parameter decreased by 31.3% (p < 0.001). The area under the receiver operating characteristic curve was 0.85 for the Nakagami-scaling parameter and was 0.95 for the Nakagami-shape parameter, which further demonstrated the diagnostic efficiency of the Nakagami parameters. Conclusions: Nakagami parameters could be used to quantitatively measure parotid-gland injury following head-and-neck radiotherapy. Moreover, the clinical feasibility was demonstrated and this study provides meaningful preliminary data for future clinical investigation.

  4. Drug safety evaluation through biomarker analysis-A toxicity study in the cynomolgus monkey using an antibody-cytotoxic conjugate against ovarian cancer

    SciTech Connect (OSTI)

    Hsieh, Frank Y. Tengstrand, Elizabeth; Lee, J.-W.; Li, Lily Y.; Silverman, Lee; Riordan, Bill; Miwa, Gerald; Milton, Mark; Alden, Carl; Lee, Frank

    2007-10-01

    Antibody-cytotoxin conjugates are complex novel therapeutic agents whose toxicological properties are not presently well understood. The objective of this study was to identify serum biomarkers that correlate with MLN8866 (an Antibody-Cytotoxic Conjugate, mAb8866-CT) pathological events in monkeys and to predict the maximal tolerated dose (MTD) level using biomarkers. Cynomolgus monkeys were administered a single dose MLN8666 (5, 15 or 30 mg/kg) by intravenous infusion and evaluated over a 7-day period. Exposure levels were determined by quantifying MLN8866 levels (C{sub max} and AUC{sub 0-96h}) in serum. The increase in MLN8866 C{sub max} and AUC{sub 0-96h} was approximately dose proportional. Two biomarkers in serum (m/z 316 and m/z 368) were identified to be correlated with MLN8866 toxicological outcomes. The predicted MTD, 11.4 mg/kg, was within the MTD range set by pathology results (5-15 mg/kg). Administration of MLN8866 at 15 mg/kg and 30 mg/kg dose levels resulted in changes in hematology parameters associated with impaired hematopoiesis and bone marrow toxicity. The projected MLN8866 MTD exposure level was integrated with toxicokinetic analysis and showed C{sub max} = 236 {mu}g/mL and AUC{sub 0-96h} = 7246 h mg/mL. The safety of three different MLN8866 dosing regimens with three dosing schedules was explored with pharmacokinetic modeling.

  5. SU-E-T-501: Normal Tissue Toxicities of Pulsed Low Dose Rate Radiotherapy and Conventional Radiotherapy: An in Vivo Total Body Irradiation Study

    SciTech Connect (OSTI)

    Cvetkovic, D; Zhang, P; Wang, B; Chen, L; Ma, C

    2014-06-01

    Purpose: Pulsed low dose rate radiotherapy (PLDR) is a re-irradiation technique for therapy of recurrent cancers. We have previously shown a significant difference in the weight and survival time between the mice treated with conventional radiotherapy (CRT) and PLDR using total body irradiation (TBI). The purpose of this study was to investigate the in vivo effects of PLDR on normal mouse tissues.Materials and Methods: Twenty two male BALB/c nude mice, 4 months of age, were randomly assigned into a PLDR group (n=10), a CRT group (n=10), and a non-irradiated control group (n=2). The Siemens Artiste accelerator with 6 MV photon beams was used. The mice received a total of 18Gy in 3 fractions with a 20day interval. The CRT group received the 6Gy dose continuously at a dose rate of 300 MU/min. The PLDR group was irradiated with 0.2Gyx20 pulses with a 3min interval between the pulses. The mice were weighed thrice weekly and sacrificed 2 weeks after the last treatment. Brain, heart, lung, liver, spleen, gastrointestinal, urinary and reproductive organs, and sternal bone marrow were removed, formalin-fixed, paraffin-embedded and stained with H and E. Morphological changes were observed under a microscope. Results: Histopathological examination revealed atrophy in several irradiated organs. The degree of atrophy was mild to moderate in the PLDR group, but severe in the CRT group. The most pronounced morphological abnormalities were in the immune and hematopoietic systems, namely spleen and bone marrow. Brain hemorrhage was seen in the CRT group, but not in the PLDR group. Conclusions: Our results showed that PLDR induced less toxicity in the normal mouse tissues than conventional radiotherapy for the same dose and regimen. Considering that PLDR produces equivalent tumor control as conventional radiotherapy, it would be a good modality for treatment of recurrent cancers.

  6. Evaluation of 2,3,7,8 specific congener and toxic potency of persistent polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in cetaceans from the Mediterranean Sea, Italy

    SciTech Connect (OSTI)

    Jimenez, B.; Gonzalez, M.J.; Jimenez, O.; Reich, S.; Eljarrat, E.; Rivera, J.

    2000-03-01

    The present study investigates individual 2,3,7,8-substituted polychlorinated dibenzo-p-dioxin (PCDD) and polychlorinated dibenzofuran (PCDF) concentrations in cetaceans and assesses the PCDD and PCDF contributions to the total TCDD equivalent concentration. Liver samples of a variety of cetaceans species found stranded along the Italian coasts during the period of 1987--1992 were analyzed for PCDDs and PCDFs. The main aims were to scrutinize levels and patterns of PCDDs and PCDFs for the first time in cetaceans from the Mediterranean Sea and to estimate whether chlorinated compounds mentioned are implicated in the exceptionally high mortality of dolphins that occurred in the Mediterranean sea during 1990--1992. PCDD and PCDF profiles were dominated by congeners OCCD and OCDF. The new toxic equivalency factors recommended by WHO in 1997 were used for calculation of dioxin-like toxicity. On the basis of their previous study of PCBs, the overall TEQ calculated from the 2,3,7,8-substituted PCDDs and PCDFs do not contribute as much dioxin-like toxicity as PCBs.

  7. Method for detecting toxic gases

    DOE Patents [OSTI]

    Stetter, Joseph R. (Naperville, IL); Zaromb, Solomon (Hinsdale, IL); Findlay, Jr., Melvin W. (Bolingbrook, IL)

    1991-01-01

    A method capable of detecting low concentrations of a pollutant or other component in air or other gas, utilizing a combination of a heating filament having a catalytic surface of a noble metal for exposure to the gas and producing a derivative chemical product from the component, and an electrochemical sensor responsive to the derivative chemical product for providing a signal indicative of the product. At concentrations in the order of about 1-100 ppm of tetrachloroethylene, neither the heating filament nor the electrochemical sensor is individually capable of sensing the pollutant. In the combination, the heating filament converts the benzyl chloride to one or more derivative chemical products which may be detected by the electrochemical sensor.

  8. Precombustion control of air toxics

    SciTech Connect (OSTI)

    Akers, D.J.; Harrison, C.; Nowak, M.; Toole-O`Neil, B.

    1996-12-31

    If regulation of hazardous air pollutant emissions from utility boilers occurs in the next few years, the least-cost, lowest-risk control method for many utilities is likely to be some form of coal cleaning. Approximately 75 percent of coal mined east of the Mississippi River is already cleaned before it is used by the electric utility industry. Current methods of coal cleaning reduce ash and sulfur content by removing ash-forming and sulfur-bearing minerals; these same methods have the capability to remove large amounts of most of the 14 elements named as hazardous air pollutants (HAPs) in Title III of the 1990 Amendments to the Clean Air Act.

  9. Method for detecting toxic gases

    DOE Patents [OSTI]

    Stetter, J.R.; Zaromb, S.; Findlay, M.W. Jr.

    1991-10-08

    A method is disclosed which is capable of detecting low concentrations of a pollutant or other component in air or other gas. This method utilizes a combination of a heating filament having a catalytic surface of a noble metal for exposure to the gas and producing a derivative chemical product from the component. An electrochemical sensor responds to the derivative chemical product for providing a signal indicative of the product. At concentrations in the order of about 1-100 ppm of tetrachloroethylene, neither the heating filament nor the electrochemical sensor is individually capable of sensing the pollutant. In the combination, the heating filament converts the benzyl chloride to one or more derivative chemical products which may be detected by the electrochemical sensor. 6 figures.

  10. Criteria and Processes for the Certification of Non-Radioactive Hazardous and Non-Hazardous Wastes

    SciTech Connect (OSTI)

    Dominick, J

    2008-12-18

    This document details Lawrence Livermore National Laboratory's (LLNL) criteria and processes for determining if potentially volumetrically contaminated or potentially surface contaminated wastes are to be managed as material containing residual radioactivity or as non-radioactive. This document updates and replaces UCRL-AR-109662, Criteria and Procedures for the Certification of Nonradioactive Hazardous Waste (Reference 1), also known as 'The Moratorium', and follows the guidance found in the U.S. Department of Energy (DOE) document, Performance Objective for Certification of Non-Radioactive Hazardous Waste (Reference 2). The 1992 Moratorium document (UCRL-AR-109662) is three volumes and 703 pages. The first volume provides an overview of the certification process and lists the key radioanalytical methods and their associated Limits of Sensitivities. Volumes Two and Three contain supporting documents and include over 30 operating procedures, QA plans, training documents and organizational charts that describe the hazardous and radioactive waste management system in place in 1992. This current document is intended to update the previous Moratorium documents and to serve as the top-tier LLNL institutional Moratorium document. The 1992 Moratorium document was restricted to certification of Resource Conservation and Recovery Act (RCRA), State and Toxic Substances Control Act (TSCA) hazardous waste from Radioactive Material Management Areas (RMMA). This still remains the primary focus of the Moratorium; however, this document increases the scope to allow use of this methodology to certify other LLNL wastes and materials destined for off-site disposal, transfer, and re-use including non-hazardous wastes and wastes generated outside of RMMAs with the potential for DOE added radioactivity. The LLNL organization that authorizes off-site transfer/disposal of a material or waste stream is responsible for implementing the requirements of this document. The LLNL Radioactive and Hazardous Waste Management (RHWM) organization is responsible for the review and maintenance of this document. It should be noted that the DOE metal recycling moratorium is still in effect and is implemented as outlined in reference 17 when metals are being dispositioned for disposal/re-use/recycling off-site. This document follows the same methodology as described in the previously approved 1992 Moratorium document. Generator knowledge and certification are the primary means of characterization. Sampling and analysis are used when there is insufficient knowledge of a waste to determine if it contains added radioactivity. Table 1 (page 12) presents a list of LLNL's analytical methods for evaluating volumetrically contaminated waste and updates the reasonably achievable analytical-method-specific Minimum Detectable Concentrations (MDCs) for various matrices. Results from sampling and analysis are compared against the maximum MDCs for the given analytical method and the sample specific MDC to determine if the sample contains DOE added volumetric radioactivity. The evaluation of an item that has a physical form, and history of use, such that accessible surfaces may be potentially contaminated, is based on DOE Order 5400.5 (Reference 3), and its associated implementation guidance document DOE G 441.1-XX, Control and Release of Property with Residual Radioactive Material (Reference 4). The guidance document was made available for use via DOE Memorandum (Reference 5). Waste and materials containing residual radioactivity transferred off-site must meet the receiving facilities Waste Acceptance Criteria (if applicable) and be in compliance with other applicable federal or state requirements.

  11. Application for a Permit to Operate a Class III Solid Waste Disposal Site at the Nevada National Security Site Area 5 Asbestiform Low-Level Solid Waste Disposal Site

    SciTech Connect (OSTI)

    NSTec Environmental Programs

    2010-10-04

    The Nevada National Security Site (NNSS) is located approximately 105 km (65 mi) northwest of Las Vegas, Nevada. The U.S. Department of Energy National Nuclear Security Administration Nevada Site Office (NNSA/NSO) is the federal lands management authority for the NNSS and National Security Technologies, LLC (NSTec) is the Management and Operations contractor. Access on and off the NNSS is tightly controlled, restricted, and guarded on a 24-hour basis. The NNSS is posted with signs along its entire perimeter. NSTec is the operator of all solid waste disposal sites on the NNSS. The Area 5 Radioactive Waste Management Site (RWMS) is the location of the permitted facility for the Solid Waste Disposal Site (SWDS). The Area 5 RWMS is located near the eastern edge of the NNSS (Figure 1), approximately 26 km (16 mi) north of Mercury, Nevada. The Area 5 RWMS is used for the disposal of low-level waste (LLW) and mixed low-level waste. Many areas surrounding the RWMS have been used in conducting nuclear tests. The site will be used for the disposal of regulated Asbestiform Low-Level Waste (ALLW), small quantities of low-level radioactive hydrocarbon-burdened (LLHB) media and debris, LLW, LLW that contains Polychlorinated Biphenyl (PCB) Bulk Product Waste greater than 50 ppm that leaches at a rate of less than 10 micrograms of PCB per liter of water, and small quantities of LLHB demolition and construction waste (hereafter called permissible waste). Waste containing free liquids, or waste that is regulated as hazardous waste under the Resource Conservation and Recovery Act (RCRA) or state-of-generation hazardous waste regulations, will not be accepted for disposal at the site. Waste regulated under the Toxic Substances Control Act (TSCA) that will be accepted at the disposal site is regulated asbestos-containing materials (RACM) and PCB Bulk Product Waste greater than 50 ppm that leaches at a rate of less than 10 micrograms of PCB per liter of water. The term asbestiform is used throughout this document to describe RACM. The disposal site will be used as a depository of permissible waste generated both on site and off site. All generators designated by NNSA/NSO will be eligible to dispose regulated ALLW at the Asbestiform Low-Level Waste Disposal Site in accordance with the DOE/NV-325, Nevada National Security Site Waste Acceptance Criteria (NNSSWAC, current revision). Approval will be given by NNSA/NSO to generators that have successfully demonstrated through process knowledge (PK) and/or sampling and analysis that the waste is low-level, contains asbestiform material, or contains PCB Bulk Product Waste greater than 50 ppm that leaches at a rate of less than 10 micrograms of PCB per liter of water, or small quantities of LLHB demolition and construction waste and does not contain prohibited waste materials. Each waste stream will be approved through the Radioactive Waste Acceptance Program (RWAP), which ensures that the waste meets acceptance requirements outlined in the NNSSWAC.

  12. Streamlined Approach for Environmental Restoration Plan for Corrective Action Unit 121: Storage Tanks and Miscellaneous Sites, Nevada Test Site, Nevada

    SciTech Connect (OSTI)

    NSTec Environmental Restoration

    2007-06-01

    This Streamlined Approach for Environmental Restoration (SAFER) Plan identifies the activities required for the closure of Corrective Action Unit (CAU) 121, Storage Tanks and Miscellaneous Sites. CAU 121 is currently listed in Appendix III of the ''Federal Facility Agreement and Consent Order'' (FFACO, 1996) and consists of three Corrective Action Sites (CASs) located in Area 12 of the Nevada Test Site (NTS): CAS 12-01-01, Aboveground Storage Tank; CAS 12-01-02, Aboveground Storage Tank; and CAS 12-22-26, Drums; 2 AST's. CASs 12-01-01 and 12-01-02 are located to the west of the Area 12 Camp, and CAS 12-22-26 is located near the U-12g Tunnel, also known as G-tunnel, in Area 12 (Figure 1). The aboveground storage tanks (ASTs) present at CASs 12-01-01 and 12-01-02 will be removed and disposed of at an appropriate facility. Soil below the ASTs will be sampled to identify whether it has been impacted with chemicals or radioactivity above action levels. If impacted soil above action levels is present, the soil will be excavated and disposed of at an appropriate facility. The CAS 12-22-26 site is composed of two overlapping areas, one where drums had formerly been stored, and the other where an AST was used to dispense diesel for locomotives used at G-tunnel. This area is located above an underground radioactive materials area (URMA), and within an area that may have elevated background radioactivity because of containment breaches during nuclear tests and associated tunnel reentry operations. CAS 12-22-26 does not include the URMA or the elevated background radioactivity. An AST that had previously been used to store liquid magnesium chloride (MgCl) was properly disposed of several years ago, and releases from this tank are not an environmental concern. The diesel AST will be removed and disposed of at an appropriate facility. Soil at the former drum area and the diesel AST area will be sampled to identify whether it has been impacted by releases, from the drums or the AST, with chemicals or radioactivity above action levels. CAS 12-22-26 has different potential closure pathways that are dependent upon the concentrations and chemicals detected. If only petroleum hydrocarbons are detected above action levels, then the area will be use-restricted. It will not be excavated because of the more significant hazard of excavating within a URMA. Similarly, polychlorinated biphenyls (PCBs) will only be excavated for concentrations of 50 parts per million (ppm) or greater, if there are no other factors that require excavation. For PCBs at concentrations above 1 ppm, the area will be use-restricted as required by Title 40, Code of Federal Regulations (CFR) Part 761 for PCBs (CFR, 2006), in the ''Toxic Substances Control Act'' (TSCA). Other chemicals at concentrations above the final action levels (FALs) will be excavated. If radioactivity is above action levels, then the soil will be excavated only to a depth of 1 foot (ft) below ground surface (bgs) and replaced with clean fill. This action is intended to remove the ''hot spot'' on the surface caused by leakage from a drum, and not to remediate the URMA.

  13. H. R. 1726: A bill to amend the Internal Revenue Code of 1986 to deny any deduction for certain oil and hazard substance cleanup costs, introduced in the US House of Representatives, One Hundred Second Congress, First Session, April 11, 1991

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    This bill was introduced into the US House of Representatives on April 11, 1991 to amend the Internal Revenue Code of 1986 to deny any deduction for certain oil and hazardous substance cleanup costs. These discharge costs will apply if the taxpayer has a complete liability defense of if the taxpayer qualifies for a liability limitation with respect to the discharge and is not liable for any punitive damages.The amendments shall apply in the case of any applicable discharge costs paid on or after January 1, 1991.

  14. MicroCantilever (MC) based Robust Sensing Approach for Controlled

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Substances | The Ames Laboratory MicroCantilever (MC) based Robust Sensing Approach for Controlled Substances FWP/Project Description: MicroCantilever (MC) based sensors can provide revolutionary sensitivity for forensic detection and identification of controlled substance, toxic species, biological molecules, and DNA matching. Current sensor systems require extensive sample preparation and/or specialized instrumentation to identify molecules of controlled substances such as cocaine with

  15. Guidance document publications list - Office of Environmental Policy and Assistance RCRA/CERCLA Division (EH-413)

    SciTech Connect (OSTI)

    1995-08-01

    This document provides a listing of Guidance Documents from the RCRA/CERCLA Division for August 1995. Documents are listed under the following categories: RCRA Guidance Manuals; RCRA Information Briefs; CERCLA Guidance Manuals; CERCLA Regulatory Bulletins; RCRA/CERCLA Guidance Manuals; TSCA Guidance Manuals; TSCA Information Briefs; and, Cross Cut Manuals.

  16. Use of porcine vaginal tissue ex-vivo to model environmental effects on vaginal mucosa to toxic shock syndrome toxin-1

    SciTech Connect (OSTI)

    Davis, Catherine C.; Baccam, Mekhine; Mantz, Mary J.; Osborn, Thomas W.; Hill, Donna R.; Squier, Christopher A.

    2014-01-15

    Menstrual toxic shock syndrome (mTSS) is a rare, recognizable, and treatable disease that has been associated with tampon use epidemiologically. It involves a confluence of microbial risk factors (Staphylococcus aureus strains that produce the superantigenTSST-1), as well as environmental characteristics of the vaginal ecosystem during menstruation and host susceptibility factors. This paper describes a series of experiments using the well-characterized model of porcine vaginal mucosa ex-vivo to assess the effect of these factors associated with tampon use on the permeability of the mucosa. The flux of radiolabeled TSST-1 and tritiated water ({sup 3}H{sub 2}O) through porcine vaginal mucosa was determined at various temperatures, after mechanical disruption of the epithelial surface by tape stripping, after treatment with surfactants or other compounds, and in the presence of microbial virulence factors. Elevated temperatures (42, 47 and 52 C) did not significantly increase flux of {sup 3}H{sub 2}O. Stripping of the epithelial layers significantly increased the flux of labeled toxin in a dose-dependent manner. Addition of benzalkonium chloride (0.1 and 0.5%) and glycerol (4%) significantly increased the flux of {sup 3}H{sub 2}O but sodium lauryl sulfate at any concentration tested did not. The flux of the labeled toxin was significantly increased in the presence of benzalkonium chloride but not Pluronic L92 and Tween 20 and significantly increased with addition of ?-hemolysin but not endotoxin. These results show that the permeability of porcine vagina ex-vivo to labeled toxin or water can be used to evaluate changes to the vaginal environment and modifications in tampon materials, and thus aid in risk assessment. - Highlights: Model assessed local effects of tampon use on vaginal mucosa. Risks were evaluated using two tracers to assess permeability in an ex vivo model. Mechanical damage to the epithelial surface increased tracer penetration. Surfactants increased penetration of tracers across vaginal mucosa. Alpha-hemolysin increased the penetration of bacterial toxin across vaginal mucosa.

  17. Individualized 3D Reconstruction of Normal Tissue Dose for Patients With Long-term Follow-up: A Step Toward Understanding Dose Risk for Late Toxicity

    SciTech Connect (OSTI)

    Ng, Angela; Brock, Kristy K.; Sharpe, Michael B.; Department of Radiation Oncology, University of Toronto, Toronto, Ontario ; Moseley, Joanne L.; Craig, Tim; Department of Radiation Oncology, University of Toronto, Toronto, Ontario ; Hodgson, David C.

    2012-11-15

    Purpose: Understanding the relationship between normal tissue dose and delayed radiation toxicity is an important component of developing more effective radiation therapy. Late outcome data are generally available only for patients who have undergone 2-dimensional (2D) treatment plans. The purpose of this study was to evaluate the accuracy of 3D normal tissue dosimetry derived from reconstructed 2D treatment plans in Hodgkin's lymphoma (HL) patients. Methods and Materials: Three-dimensional lung, heart, and breast volumes were reconstructed from 2D planning radiographs for HL patients who received mediastinal radiation therapy. For each organ, a reference 3D organ was modified with patient-specific structural information, using deformable image processing software. Radiation therapy plans were reconstructed by applying treatment parameters obtained from patient records to the reconstructed 3D volumes. For each reconstructed organ mean dose (D{sub mean}) and volumes covered by at least 5 Gy (V{sub 5}) and 20Gy (V{sub 20}) were calculated. This process was performed for 15 patients who had both 2D and 3D planning data available to compare the reconstructed normal tissue doses with those derived from the primary CT planning data and also for 10 historically treated patients with only 2D imaging available. Results: For patients with 3D planning data, the normal tissue doses could be reconstructed accurately using 2D planning data. Median differences in D{sub mean} between reconstructed and actual plans were 0.18 Gy (lungs), -0.15 Gy (heart), and 0.30 Gy (breasts). Median difference in V{sub 5} and V{sub 20} were less than 2% for each organ. Reconstructed 3D dosimetry was substantially higher in historical mantle-field treatments than contemporary involved-field mediastinal treatments: average D{sub mean} values were 15.2 Gy vs 10.6 Gy (lungs), 27.0 Gy vs 14.3 Gy (heart), and 8.0 Gy vs 3.2 Gy (breasts). Conclusions: Three-dimensional reconstruction of absorbed dose to organs at risk can be estimated accurately many years after exposure by using limited 2D data. Compared to contemporary involved-field treatments, normal tissue doses were significantly higher in historical mantle-field treatments. These methods build capacity to quantify the relationship between 3D normal tissue dose and observed late effects.

  18. Advocate- Issue 50- April 2013

    Broader source: Energy.gov [DOE]

    Here are some stories from this issue: Legacy Waste on the Oak Ridge Reservation, K-25's North Tower Demolished, Agency for Toxic Substances and Disease Registry Meets in Oak Ridge, and a Profile on Dave Hemelright.

  19. Soil Gas Sampling | Open Energy Information

    Open Energy Info (EERE)

    "normal" background soil gas contents of a particular geothermal area. References Toxic Substances Hydrology Program 2.0 2.1 The Application of Soil-Gas Technique to...

  20. 15. international conference on plant growth substances: Program -- Abstracts

    SciTech Connect (OSTI)

    1995-12-31

    Since the 14th Conference in Amsterdam in 1991, progress in plant hormone research and developmental plant biology has been truly astonishing. The five ``classical`` plant hormones, auxin, gibberellin, cytokinin, ethylene, and abscisic acid, have been joined by a number of new signal molecules, e.g., systemin, jasmonic acid, salicylic acid, whose biosynthesis and functions are being understood in ever greater detail. Molecular genetics has opened new vistas in an understanding of transduction pathways that regulate developmental processes in response to hormonal and environmental signals. The program of the 15th Conference includes accounts of this progress and brings together scientists whose work focuses on physiological, biochemical, and chemical aspects of plant growth regulation. This volume contains the abstracts of papers presented at this conference.

  1. Microwaving of normally opaque and semi-opaque substances

    DOE Patents [OSTI]

    Sheinberg, H.; Meek, T.T.; Blake, R.D.

    1990-07-17

    Disclosed is a method of heating small particles using microwave radiation which are not normally capable of being heated by microwaves. The surfaces of the particles are coated with a material which is transparent to microwave radiation in order to cause microwave coupling to the particles and thus accomplish heating of the particles.

  2. Method and apparatus for optimized sampling of volatilizable target substances

    DOE Patents [OSTI]

    Lindgren, Eric R.; Phelan, James M.

    2004-10-12

    An apparatus for capturing, from gases such as soil gas, target analytes. Target analytes may include emanations from explosive materials or from residues of explosive materials. The apparatus employs principles of sorption common to solid phase microextraction, and is best used in conjunction with analysis means such as a gas chromatograph. To sorb target analytes, the apparatus functions using various sorptive structures to capture target analyte. Depending upon the embodiment, those structures may include a capillary tube including an interior surface on which sorptive material (similar to that on the surface of a SPME fiber) is supported (along with means for moving gases through the capillary tube so that the gases come into close proximity to the sorptive material). In one disclosed embodiment, at least one such sorptive structure is associated with an enclosure including an opening in communication with the surface of a soil region potentially contaminated with buried explosive material such as unexploded ordnance. Emanations from explosive materials can pass into and accumulate in the enclosure where they are sorbed by the sorptive structures. Also disclosed is the use of heating means such as microwave horns to drive target analytes into the soil gas from solid and liquid phase components of the soil.

  3. Method and apparatus for optimized sampling of volatilizable target substances

    DOE Patents [OSTI]

    Lindgren, Eric R. (Albuquerque, NM); Phelan, James M. (Bosque Farms, NM)

    2002-01-01

    An apparatus for capturing, from gases such as soil gas, target analytes. Target analytes may include emanations from explosive materials or from residues of explosive materials. The apparatus employs principles of sorption common to solid phase microextraction, and is best used in conjunction with analysis means such as a gas chromatograph. To sorb target analytes, the apparatus functions using various sorptive structures to capture target analyte. Depending upon the embodiment, those structures may include 1) a conventional solid-phase microextraction (SPME) fiber, 2) a SPME fiber suspended in a capillary tube (with means provided for moving gases through the capillary tube so that the gases come into close proximity to the suspended fiber), and 3) a capillary tube including an interior surface on which sorptive material (similar to that on the surface of a SPME fiber) is supported (along with means for moving gases through the capillary tube so that the gases come into close proximity to the sorptive material). In one disclosed embodiment, at least one such sorptive structure is associated with an enclosure including an opening in communication with the surface of a soil region potentially contaminated with buried explosive material such as unexploded ordnance. Emanations from explosive materials can pass into and accumulate in the enclosure where they are sorbed by the sorptive structures. Also disclosed is the use of heating means such as microwave horns to drive target analytes into the soil gas from solid and liquid phase components of the soil.

  4. Sensor array for toxic gas detection

    DOE Patents [OSTI]

    Stetter, Joseph R. (Naperville, IL); Zaromb, Solomon (Hinsdale, IL); Penrose, William R. (Naperville, IL)

    1987-01-01

    A portable instrument for use in the field in detecting and identifying a hazardous component in air or other gas including an array of small sensors which upon exposure to the gas from a pattern of electrical responses, a source of standard response patterns characteristic of various components, and microprocessor means for comparing the sensor-formed response pattern with one or more standard patterns to thereby identify the component on a display. The number of responses may be increased beyond the number of sensors by changing the operating voltage, temperature or other condition associated with one or more sensors to provide a plurality of responses from each of one or more of the sensors. In one embodiment, the instrument is capable of identifying anyone of over 50-100 hazardous components.

  5. How information resources are used by federal agencies in risk assessment application: Rapporteur summary

    SciTech Connect (OSTI)

    Fenner-Crisp, P.

    1990-12-31

    The application of information available for risk assessment from the federal perspective is described. Different federal agencies conduct varying degrees of hazard evaluation, and some also generate empirical data. The role of the Agency for Toxic Substances and Disease Registry in hazard assessments of potential public health impacts of Superfund sites includes identification of the 275 most significant substances. ATSDR is responsible for preparing toxicological profiles. ATSDR also identifies data gaps and needs critical to adequately assessing human health impacts.

  6. Microsoft Word - S06584StSoil.doc

    Office of Legacy Management (LM)

    University of Arizona Letter This page intentionally left blank December 17, 2009 Memo to: Jody Waugh Regarding: Uranium in Former Evaporation Pond Area at Monument Valley We analyzed the stained surface soils in the former evaporation pond area and extended field west in the subpile soil area for a suite of heavy metals to determine if potential toxic substances were associated with the chemical stains observed in some areas of the site. This was part of Task 6, Mn Toxicity Field Study. Our

  7. Procedural guidance for reviewing exposure information under RCRA (Resource Conservation and Recovery Act) section 3019. Final report

    SciTech Connect (OSTI)

    Grogan, T.; Kayser, R.

    1986-09-26

    This guidance manual describes the procedures for permit writers in evaluating exposure information submitted under Resource Conservation and Recovery Act (RCRA) Section 3019. The integration of the review with existing RCRA permitting activities is also discussed. The document outlines procedures to follow in referring sites to the Agency for Toxic Substances and Disease Registry (ATSDR) for health evaluations.

  8. Environmental Sciences Division annual progress report for period ending September 30, 1981

    SciTech Connect (OSTI)

    Auerbach, S.I.; Reichle, D.E.

    1982-04-01

    Research programs from the following sections and programs are summarized: aquatic ecology, environmental resources, earth sciences, terrestrial ecology, advanced fossil energy program, toxic substances program, environmental impacts program, biomass, low-level waste research and development program, US DOE low-level waste management program, and waste isolation program.

  9. In the forefront of the fight for our environment

    SciTech Connect (OSTI)

    Not Available

    1990-01-01

    This booklet publicizes the work of the Quebec Regional Office of Environment Canada's Environmental Protection Branch. It answers the most frequently asked questions on enforcing the law; controlling toxic substances, water and air pollution; Environment Canada's responsibilities in waste management and contaminated sites; and supporting the St. Lawrence action plan.

  10. Refinery siting workbook: appendices C to O

    SciTech Connect (OSTI)

    Not Available

    1980-07-01

    Applicable laws and permits available for the selection and building of petroleum refineries are enclosed. A glossary of pertinent terms is also included. References related to the National Environmental Policy Act, the Clean Air Act, the Federal Water Pollution Control Act, Resource Conservation and Recovery Act, Toxic Substance Control Act, and Wetlands and Coastal Zone are included. Permit information is also presented. (DC)

  11. Environmental Sciences Division annual progress report for period ending September 30, 1982. Environmental Sciences Division Publication No. 2090. [Lead abstract

    SciTech Connect (OSTI)

    Not Available

    1983-04-01

    Separate abstracts were prepared for 12 of the 14 sections of the Environmental Sciences Division annual progress report. The other 2 sections deal with educational activities. The programs discussed deal with advanced fuel energy, toxic substances, environmental impacts of various energy technologies, biomass, low-level radioactive waste management, the global carbon cycle, and aquatic and terrestrial ecology. (KRM)

  12. Relationship Between Composition and Toxicity of Engine Emissions...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Effect of Changes in Diesel Exhaust ... for the Health Effects of Inhaled Engine Emissions Assessment of Health Hazards of Repeated Inhalation of Diesel Emissions, with ...

  13. Method for screening inhibitors of the toxicity of Bacillus anthracis

    DOE Patents [OSTI]

    Cirino, Nick M. (Los Alamos, NM); Jackson, Paul J. (Los Alamos, NM); Lehnert, Bruce E. (Los Alamos, NM)

    2001-01-01

    The protective antigen (PA) of Bacillus anthracis is integral to the mechanism of anthrax poisoning. The cloning, expression and purification of a 32 kDa B. anthracis PA fragment (PA32) is described. This fragment has also been expressed as a fusion construct to stabilized green fluorescent protein (EGFP-PA32). Both proteins were capable of binding to specific cell surface receptors as determined by fluorescent microscopy and a flow cytometric assay. To confirm binding specificity in the flow cytometric assay, non-fluorescent PA83 or PA32 was used to competitively inhibit fluorescent EGFP-PA32 binding to cell receptors. This assay can be employed as a rapid screen for compounds which disrupts binding of PA to cells. Additionally, the high intracellular expression levels and ease of purification make this recombinant protein an attractive vaccine candidate or therapeutic treatment for anthrax poisoning.

  14. Comparative Developmental Toxicity of Environmentally Relevant Oxygenated PAHs

    SciTech Connect (OSTI)

    Knecht, Andrea; Goodale, Britton; Truong, Lisa; Simonich, Michael; Swanson, Annika; Matzke, Melissa M.; Anderson, Kim A.; Waters, Katrina M.; Tanguay, Robert

    2013-09-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants in urban air, dust and in the soil of most industrial coal gassification, coal burning, coke production and wood preservation sites (Howsam and Jones 1998). It is widely recognized that PAHs pose risks to human health,having been associated with increased risks of systemic inflammation (Delfino et al. 2010), cardiopulmonary mortality (Lee et al. 2011; Lewtas 2007) and lung cancer mortality (Grant 2009; Hoshuyama et al. 2006). The potential risks may be especially acute for the developing fetus and infant where PAH exposures have been linked to low birth weight, intrauterine growth retardation, in-utero mortality and lower intelligence (Dejmek et al. 1999; Dejmek et al. 2000; Perera et al. 1999; Perera et al. 2009; Perera et al. 2006; Perera et al. 1998; Wu et al. 2010). Despite the more than two decades of intensive study devoted to parent PAHs, they are only part of the hazard spectrum from PAH contamination.

  15. Method and apparatus for enhanced detection of toxic agents

    DOE Patents [OSTI]

    Greenbaum, Elias (Knoxville, TN); Rodriguez, Jr., Miguel (Oak Ridge, TN); Wu, Jie Jayne (Knoxville, TN); Qi, Hairong (Knoxville, TN)

    2012-06-12

    A water quality analyzer for real-time detection according to the invention comprises a biased AC electro-osmosis (ACEO) cell for receiving a fluid to be analyzed having a plurality photosynthetic organisms therein, and concentrating the plurality photosynthetic organisms into at least one concentrated region. A photodetector is provided for obtaining a measured photosynthetic activity of the plurality of photosynthetic organisms in the concentrated region, wherein chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. An electronics package analyzes the measured photosynthetic activity to indicate a presence of the chemical, biological or radiological agents in the fluid.

  16. Catalytic process for removing toxic gases from gas streams

    SciTech Connect (OSTI)

    Baglio, J.A.; Gaudet, G.G.; Palilla, F.C.

    1983-02-22

    A multi-stage process for reducing the content of sulfurcontaining gases-notably hydrogen sulfide, sulfur dioxide, carbonyl sulfide and carbon disulfide-in waste gas streams is provided. In the first stage, the gas stream is passed through a reaction zone at a temperature between about 150 and 350/sup 0/C in the presence of a pretreated novel catalyst of the formula xLn/sub 2/O/sub 3/ in which Ln is yttrium or a rare earth element and T is cobalt, iron or nickel, and each of x and y is independently a number from 0 to 3, said catalyst being substantially non-crystalline and having a surface area of from about 10 m/sup 2//g to about 40 m/sup 2//g. The preferred catalyst is one in which Ln is lanthanum, T is cobalt, and x and y range from 1 to 3, including non-integers. The first stage yields a product stream having a reduced content of sulfur-containing gases, including specifically, substantial reduction of carbonyl sulfide and virtual elimination of carbon disulfide. An intermediate stage is a claus reaction, which may take place in one or more reaction zones, at temperatures less than about 130/sup 0/ C, in the presence of known catalysts such as bauxite, alumina or cobalt molybdates. The final stage is the air oxidation of hydrogen sulfide at a temperature between about 150 and 300/sup 0/ C in the presence of a catalyst usable in first stage.

  17. Method and apparatus for enhanced detection of toxic agents

    DOE Patents [OSTI]

    Greenbaum, Elias; Rodriguez, Jr., Miguel; Wu, Jie Jayne; Qi, Hairong

    2013-10-01

    A biosensor based detection of toxins includes enhancing a fluorescence signal by concentrating a plurality of photosynthetic organisms in a fluid into a concentrated region using biased AC electro-osmosis. A measured photosynthetic activity of the photosynthetic organisms is obtained in the concentrated region, where chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. A presence of the chemical, biological and/or radiological agents or precursors thereof, is determined in the fluid based on the measured photosynthetic activity of the concentrated plurality of photosynthetic organisms. A lab-on-a-chip system is used for the concentrating step. The presence of agents is determined from feature vectors, obtained from processing a time dependent signal using amplitude statistics and/or time-frequency analysis, relative to a control signal. A linear discriminant method including support vector machine classification (SVM) is used to identify the agents.

  18. On emissions trading, toxic debt and the Australian power market

    SciTech Connect (OSTI)

    Simshauser, Paul

    2009-03-15

    Implementation of emissions trading will have profound effects on the financial stability of coal generators. While the impact on equity capital is well understood, the potential fallout in the market for project finance is not. During the current global financial crisis, the form and quantum of transitional assistance to coal generators will be crucial to ensure ongoing participation of domestic and foreign project banks in the power markets. (author)

  19. Enhanced Detection of Toxic Agents - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of chemical, biological, or radiological agents in the fluid. Integration of biased AC electroosmosis with the AquaSentinel technology provides earlier detection than...

  20. Reproductive toxicity of low-level lead exposure in men

    SciTech Connect (OSTI)

    Telisman, Spomenka Colak, Bozo; Pizent, Alica; Jurasovic, Jasna; Cvitkovic, Petar

    2007-10-15

    Parameters of semen quality, seminal plasma indicators of secretory function of the prostate and seminal vesicles, sex hormones in serum, and biomarkers of lead, cadmium, copper, zinc, and selenium body burden were measured in 240 Croatian men 19-52 years of age. The subjects had no occupational exposure to metals and no known other reasons suspected of influencing male reproductive function or metal metabolism. After adjusting for age, smoking, alcohol, blood cadmium, and serum copper, zinc, and selenium by multiple regression, significant (P<0.05) associations of blood lead (BPb), {delta}-aminolevulinic acid dehydratase (ALAD), and/or erythrocyte protoporphyrin (EP) with reproductive parameters indicated a lead-related increase in immature sperm concentration, in percentages of pathologic sperm, wide sperm, round sperm, and short sperm, in serum levels of testosterone and estradiol, and a decrease in seminal plasma zinc and in serum prolactin. These reproductive effects were observed at low-level lead exposure (BPb median 49 {mu}g/L, range 11-149 {mu}g/L in the 240 subjects) common for general populations worldwide. The observed significant synergistic effect of BPb and blood cadmium on increasing serum testosterone, and additive effect of a decrease in serum selenium on increasing serum testosterone, may have implications on the initiation and development of prostate cancer because testosterone augments the progress of prostate cancer in its early stages.

  1. Sol-gel deposition of buffer layers on biaxially textured metal substances

    DOE Patents [OSTI]

    Shoup, Shara S. (Woodstock, GA); Paranthamam, Mariappan (Knoxville, TN); Beach, David B. (Knoxville, TN); Kroeger, Donald M. (Knoxville, TN); Goyal, Amit (Knoxville, TN)

    2000-01-01

    A method is disclosed for forming a biaxially textured buffer layer on a biaxially oriented metal substrate by using a sol-gel coating technique followed by pyrolyzing/annealing in a reducing atmosphere. This method is advantageous for providing substrates for depositing electronically active materials thereon.

  2. Multiple sample characterization of coals and other substances by controlled-atmosphere programmed temperature oxidation

    DOE Patents [OSTI]

    LaCount, Robert B.

    1993-01-01

    A furnace with two hot zones holds multiple analysis tubes. Each tube has a separable sample-packing section positioned in the first hot zone and a catalyst-packing section positioned in the second hot zone. A mass flow controller is connected to an inlet of each sample tube, and gas is supplied to the mass flow controller. Oxygen is supplied through a mass flow controller to each tube to either or both of an inlet of the first tube and an intermediate portion between the tube sections to intermingle with and oxidize the entrained gases evolved from the sample. Oxidation of those gases is completed in the catalyst in each second tube section. A thermocouple within a sample reduces furnace temperature when an exothermic condition is sensed within the sample. Oxidized gases flow from outlets of the tubes to individual gas cells. The cells are sequentially aligned with an infrared detector, which senses the composition and quantities of the gas components. Each elongated cell is tapered inward toward the center from cell windows at the ends. Volume is reduced from a conventional cell, while permitting maximum interaction of gas with the light beam. Reduced volume and angulation of the cell inlets provide rapid purgings of the cell, providing shorter cycles between detections. For coal and other high molecular weight samples, from 50% to 100% oxygen is introduced to the tubes.

  3. Contractor Workplace Substance Abuse Program at DOE Sites (10 CFR 707)

    Broader source: Energy.gov [DOE]

    This rule establishes policies, criteria, and procedures for developing and implementing programs to maintain a workplace free from the use of illegal drugs. It applies to DOE contractors performing work at sites owned or controlled by DOE and to individuals with unescorted access to the control areas of certain DOE reactors.

  4. Separation of a target substance from a fluid or mixture using...

    Office of Scientific and Technical Information (OSTI)

    Roger D ; Spadaccini, Christopher M ; Stolaroff, Joshuah K ; Bourcier, William L ; Lewis, Jennifer A ; Duoss, Eric B ; Vericella, John J Publication Date: 2014-09-16 OSTI...

  5. Safety Requirements for the Packaging and Transportation of Hazardous Materials, Hazardous Substances, and Hazardous Wastes

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1985-07-09

    Cancels Chapter 3 of DOE 5480.1A. Canceled by DOE O 460.1 of 9-27-1995 and by DOE N 251.4 & Para. 9c canceled by DOE O 231.1 of 9-30-1995.

  6. Annual research plan, 1983-84. [Organic compounds derived from fossil substances

    SciTech Connect (OSTI)

    1984-05-01

    The National Institute for Petroleum and Energy Research (NIPER) resulted from efforts by the Department of Energy (DOE) to ensure the continuity of the unique energy research capabilities that had been developed at the Bartlesville Energy Technology Center (BETC) over the past 65 years. This was accomplished by a Cooperative Agreement between DOE and IIT Research Institute (IITRI). The agreement to operate NIPER for the five fiscal years 1984-88 became effective October 1, 1983. The NIPER Annual Research Plan for 1983-84 consists of eight projects in the Base Program and 13 projects in the Optional Program. A sampling of potential Work for Others projects is also presented. The Base Program consists of five EOR and three Fundamental Petroleum Chemistry projects. The Optional Program has three EOR projects, one Unconventional Gas Recovery project, five APT projects, and four Advanced Utilization Research projects.

  7. Surface preparation of substances for continuous convective assembly of fine particles

    DOE Patents [OSTI]

    Rossi, Robert (Rochester, MN)

    2003-01-01

    A method for producing periodic nanometer-scale arrays of metal or semiconductor junctions on a clean semiconductor substrate surface is provided comprising the steps of: etching the substrate surface to make it hydrophilic, forming, under an inert atmosphere, a crystalline colloid layer on the substrate surface, depositing a metal or semiconductor material through the colloid layer onto the surface of the substrate, and removing the colloid from the substrate surface. The colloid layer is grown on the clean semiconductor surface by withdrawing the semiconductor substrate from a sol of colloid particles.

  8. Laboratory for Energy-Related Health Research Compliance Order, October 6, 1995

    Office of Environmental Management (EM)

    Laboratory for Energy-Related Health Research (LEHR) Compliance Order, October 6, 1.. Page 1 of 11 EM Home | Regulatory Compliance | Environmental Compliance Agreements Laboratory for Energy-Related Health Research (LEHR) Compliance Order, October 6, 1995 STATE OF CALIFORNIA ENVIRONMENTAL PROTECTION AGENCY DEPARTMENT OF TOXIC SUBSTANCES CONTROL _________________________________ In the Matter of: LABORATORY FOR ENERGY-RELATED HEALTH RESEARCH (LEHR). HWCA U.S. DEPARTMENT OF ENERGY, Respondent. ) )

  9. Lawrence Berkeley National Laboratory Compliance Order, October 6, 1995 Summary

    Office of Environmental Management (EM)

    Berkeley National Laboratory Agreement Name Lawrence Berkeley National Laboratory Compliance Order, October 6, 1995 HWCA # 95/96-016 State California Agreement Type Compliance Agreement Legal Driver(s) FFCAct Scope Summary Address LDR requirements pertaining to storage and treatment of covered waste at LBNL Parties DOE; State of California Environmental Protection Agency (Department of Toxic Substances Control) Date 10/6/1995 SCOPE * Address LDR requirements pertaining to storage and treatment

  10. Lawrence Livermore National Laboratory Federal Facility Compliance Order, February 24, 1997 Summary

    Office of Environmental Management (EM)

    Federal Facility Compliance Act Order for Lawrence Livermore National Laboratory Compliance Order HWCA 96/97-5002 State California Agreement Type Federal Facility Agreement Legal Driver(s) FFCAct Scope Summary Require compliance by the DOE with a Site Treatment Plan for the treatment of mixed waste at Lawrence Livermore National Laboratory Parties DOE; State of California Environmental Protection Agency (Department of Toxic Substances Control) Date 2/24/1997 SCOPE * Require compliance by the DOE

  11. EEOICPA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    You are here: DOE-ID Home > Energy Employees' Compensation Program INL Communication Energy Employees Occupational Illness Compensation Program Department of Labor Logo During the period of the Cold War, thousands of workers were employed in the nation's atomic weapons programs. The work was dangerous and consequently many workers may have been exposed to radioactive and toxic substances. Recognizing that workers at these facilities may be suffering from illnesses caused by their work,

  12. Structural analysis of thermostabilizing mutations of cocaine esterase

    Office of Scientific and Technical Information (OSTI)

    (Journal Article) | SciTech Connect Structural analysis of thermostabilizing mutations of cocaine esterase Citation Details In-Document Search Title: Structural analysis of thermostabilizing mutations of cocaine esterase Cocaine is considered to be the most addictive of all substances of abuse and mediates its effects by inhibiting monoamine transporters, primarily the dopamine transporters. There are currently no small molecules that can be used to combat its toxic and addictive properties,

  13. Microsoft Word - S05993_CY2009 Annual Rpt.doc

    Office of Legacy Management (LM)

    4-1 4.0 References ATSDR (Agency for Toxic Substances and Disease Registry), 2007. Analyses of Groundwater Flow, Contaminant Fate and Transport, and Distribution of Drinking Water at Tarawa Terrace and Vicinity, U.S. Marine Corps Base Camp Lejeune, North Carolina: Historical Reconstruction and Present Day Conditions, Chapter D, "Properties and Degradation Pathways of Common Organic Compounds in Groundwater," Atlanta, Georgia, September. Carter, R.W. and J. Davidian, 1968. General

  14. Microsoft Word - S07121_CY2010 Annual Rpt

    Office of Legacy Management (LM)

    401 4.0 References ATSDR (Agency for Toxic Substances and Disease Registry), 2007. Analyses of Groundwater Flow, Contaminant Fate and Transport, and Distribution of Drinking Water at Tarawa Terrace and Vicinity, U.S. Marine Corps Base Camp Lejeune, North Carolina: Historical Reconstruction and Present Day Conditions, Chapter D, "Properties and Degradation Pathways of Common Organic Compounds in Groundwater," Atlanta, Georgia, September. Burke, Patricia M., Steven Hill, Nenad Iricanin,

  15. Microsoft Word - S07834_2011 Ann Rpt_ag comment revs

    Office of Legacy Management (LM)

    7 6.0 References ATSDR (Agency for Toxic Substances and Disease Registry), 2004. Toxicological Profile for Strontium, U.S. Department of Health and Human Services, Public Health Service, April. DOE (U.S. Department of Energy), 1994. Baseline Risk Assessment of Ground Water Contamination at the Uranium Mill Tailings Site at Shiprock, New Mexico, DOE/AL/62350-48F, Rev. 1, Albuquerque Operations Office, Albuquerque, New Mexico, April. DOE (U.S. Department of Energy), 2000. Final Site Observational

  16. Microsoft Word - S08568_CY2011 Annual Rpt

    Office of Legacy Management (LM)

    423 4.0 References ATSDR (Agency for Toxic Substances and Disease Registry), 2007. Analyses of Groundwater Flow, Contaminant Fate and Transport, and Distribution of Drinking Water at Tarawa Terrace and Vicinity, U.S. Marine Corps Base Camp Lejeune, North Carolina: Historical Reconstruction and Present Day Conditions, Chapter D, "Properties and Degradation Pathways of Common Organic Compounds in Groundwater," Atlanta, Georgia, September. Carter, R.W. and J. Davidian, 1968. General

  17. Microsoft Word - S09641_2012Annual.docx

    Office of Legacy Management (LM)

    439 4.0 References ATSDR (Agency for Toxic Substances and Disease Registry), 2007. Analyses of Groundwater Flow, Contaminant Fate and Transport, and Distribution of Drinking Water at Tarawa Terrace and Vicinity, U.S. Marine Corps Base Camp Lejeune, North Carolina: Historical Reconstruction and Present Day Conditions, Chapter D, "Properties and Degradation Pathways of Common Organic Compounds in Groundwater," Atlanta, Georgia, September. Carter, R.W. and J. Davidian, 1968. General

  18. Microsoft Word - S11432_2013Annual Rpt.docx

    Office of Legacy Management (LM)

    425 4.0 References Adrian, L. and H. Gorisch, 2002." Microbial transformation of chlorinated benzenes under anaerobic conditions," in Research in Microbiology 153, pp. 131-137; Elsevier, February 14. ATSDR (Agency for Toxic Substances and Disease Registry), 2007. Analyses of Groundwater Flow, Contaminant Fate and Transport, and Distribution of Drinking Water at Tarawa Terrace and Vicinity, U.S. Marine Corps Base Camp Lejeune, North Carolina: Historical Reconstruction and Present Day

  19. Paducah | Department of Energy

    Energy Savers [EERE]

    Paducah Paducah Below are compliance agreements for the Paducah site, and summaries of the agreements are included. These agreements help in promoting cooperation between EM, Department organizations, and state agencies. PDF icon Federal Facility Agreement for the Paducah Gaseous Diffusion Plant PDF icon Federal Facility Agreement for the Paducah Gaseous Diffusion Plant Summary PDF icon Toxic Substances Control Act Uranium Enrichment Federal Facilities Compliance Agreement, February 20, 1992 PDF

  20. Job Safety

    Office of Environmental Management (EM)

    Job Safety and Health It's the law! EMPLOYEES: Must have access to:  DOE safety and health publications;  The worker safety and health program for their location;  This safety and health poster;  Copies of their medical records and records of their exposures to toxic and harmful substances or conditions; and  Results of inspections and accident investigations. Must be able to:  Express concerns related to worker safety and health;  Decline to perform an assigned task because

  1. Grant Awarded to California for Oversight of Santa Susana Field Laboratory

    Office of Environmental Management (EM)

    Cleanup | Department of Energy Contacts Bill Taylor bill.taylor@srs.gov 803-952-8564 Cincinnati - The Department of Energy today awarded a grant to the State of California Department of Toxic Substance Control (DTSC) for regulatory functions necessary to oversee investigation and cleanup at the Energy Technology Engineering Center (ETEC) project at the Santa Susana Field Laboratory (SSFL), Canoga Park, California. The $5.6 million grant has five one-year performance periods. DOE must comply

  2. Grant Awarded to California for Oversight of Santa Susana Field Laboratory

    Office of Environmental Management (EM)

    Cleanup | Department of Energy Contact Bill Taylor, 803-952-8564 bill.taylor@srs.gov Cincinnati - The Department of Energy today awarded a grant to the State of California Department of Toxic Substance Control (DTSC) for regulatory functions necessary to oversee investigation and cleanup at the Energy Technology Engineering Center (ETEC) project at the Santa Susana Field Laboratory (SSFL), Canoga Park, California. The $5.6 million grant has five one-year performance periods. DOE must comply

  3. DRAFT REMEDIAL DESIGN REPORT/REMEDIAL ACTION WORK PLAN

    Office of Legacy Management (LM)

    5 Annual Land-Use Covenant Inspection Report for DOE Areas at the Laboratory for Energy-Related Health Research/Old Campus Landfill Superfund Site University of California, Davis January 2016 LMS/LEH/S13715 This page intentionally left blank REPORT DISTRIBUTION John Bystra State of California Department of Toxic Substances Control 8800 Cal Center Drive Sacramento, CA 95826 Scott Surovchak U.S. Department of Energy Office of Legacy Management 11025 Dover Street, Suite 1000 Westminster, CO 80021

  4. Microsoft Word - S12552_2014_Annual_LUC_Report_elh.docx

    Office of Legacy Management (LM)

    Land-Use Covenant Inspection Report for DOE Areas at the Laboratory for Energy-Related Health Research/Old Campus Landfill Superfund Site University of California, Davis January 2015 LMS/LEH/S12552 This page intentionally left blank REPORT DISTRIBUTION John Bystra State of California Department of Toxic Substances Control 8800 Cal Center Drive Sacramento, CA 95826 Cliff Carpenter U. S. Department of Energy Office of Legacy Management 99 Research Park Road Morgantown, WV 26505 Sue Fields

  5. EPIcode | Department of Energy

    Office of Environmental Management (EM)

    EPIcode EPIcode Central Registry Toolbox Code Version(s): V7.0 Code Owner: Homann Associates, Inc. Description: The Emergency Prediction Information code, or EPIcode, program was developed to provide emergency response personnel and emergency planners with a software tool to help evaluate the atmospheric release of toxic substances. EPIcode allows fast estimation and assessment of chemical release scenarios associated with accidents from industry and transportation. The software can also be used

  6. Microsoft Word - 01a - Cover.doc

    National Nuclear Security Administration (NNSA)

    Environmental Assessment for Increased Depleted Uranium Use on Target 63-10, Nevada Test and Training Range Prepared for Headquarters Air Combat Command and Nellis Air Force Base, NV September 2006 ACRONYMS AND ABBREVIATIONS AFB Air Force Base AFI Air Force Instruction AFIOH Air Force Institute for Operational Health Air Force United States Air Force API Armor-Piercing Incendiary ATSDR Agency for Toxic Substances and Disease Registry BRAC Base Realignment and Closure CAA Clean Air Act CAF Combat

  7. A laboratory approach for determining the effect of oils and dispersants on mangroves

    SciTech Connect (OSTI)

    Baca, B.J.

    1982-10-01

    An experimental approach was developed and applied to testing the effects of oil and dispersant combinations on the growth of mangrove seedlings (trees of the intertidal tropics). A controlled growth chamber was employed to test the effects of different oils and dispersed oils in an array of dosages applied to different parts of the plants. Preliminary test results are reported for two species of mangroves collected from five localities, including both oiled and unoiled estuaries. Differences occurred between species, substances, dosages, the part of the plant dosed, and the presence of chronic oil pollution at localities from which the stocks were collected. Avicennia germinans (L.) L. (black mangrove) was more sensitive than Rhizophora mangle L. (red mangrove) when exposed to almost all substances tested. Light Arabian crude oil (LA) and light Arabian crude oil dispersed (LAD) were the most toxic substances tested. No. 2 fuel oil (N2) and No. 2 fuel oil dispersed (N2D) were as toxic as LA and LAD, except for an increase (an enhancement effect) in foliage and stem growth in Avicennia at lower dosages. Bunker C oil (BC) was the least toxic of the oils tested, resulting in the reduction of foliage and stem growth only at the highest dosage tested in Avicennia. Bunker C oil dispersed (BCD) failed to show effects in either species at any dosage tested. The leaves of Rhizophora were the most sensitive part of the plant tested.

  8. Application and results of whole-body autoradiography in distribution studies of organic solvents

    SciTech Connect (OSTI)

    Bergman, K.

    1983-01-01

    With the growing concern for the health hazards of occupational exposure to toxic substances attention has been focused on the organic solvents, which are associated with both deleterious nervous system effects and specific tissue injuries. Relatively little is known about the distribution of organic solvents and their metabolites in the living organism. Knowledge of the specific tissue localizations and retention of solvents and solvent metabolites is of great value in revealing and understanding the sites and mechanisms of organic solvent toxicity. Whole-body autoradiography has been modified and applied to distribution studies of benzene, toluene, m-xylene, styrene, methylene chloride, chloroform, carbon tetrachloride, trichloroethylene and carbon disulfide. The high volatility of these substances has led to the development of cryo-techniques. Whole-body autoradiographic techniques applicable to the study of volatile substances are reviewed. The localizations of nonvolatile solvent metabolites and firmly bound metabolites have also been examined. The obtained results are discussed in relation to toxic effects and evaluated by comparison with other techniques used in distribution studies of organic solvents and their metabolites.

  9. The flux and recovery of bioactive substances in the surface sediments of deep basins off southern California

    SciTech Connect (OSTI)

    Jahnke, R.A.

    1990-06-11

    Sediment microbial community biomass and activity in Santa Monica Basin, a nearshore basin in the California Continental Borderland, were examined in October 1985, 1986 and 1987, May 1986, April 1987 and January 1990. Millimeter-scale ATP profiles and incubation of intact cores with {sup 3}H-adenine indicated a high-biomass interface microbial population in the low-oxygen central basin, which was absent in samples from the basin slope sediments. A majority of microbial activity and organic matter mineralization occurred in the top cm of sediment. Comparison of measured ATP and total organic carbon profiles suggest that the C:ATP ratio (wt:wt) ranges between 47:1 and 77:1 in central basin interfacial populations, substantially lower than reported for other aquatic environments. Carbon production estimated from DNA synthesis measurements via {sup 3}H-adenine incorporation was compared with TCO{sub 2} fluxes measured by in situ benthic chamber experiments. Within the uncertainty of the C:ATP ratio, an overall microbial carbon assimilation efficiency of 75--90% was indicated. The low C:ATP ratios and high carbon assimilation efficiencies significantly affect estimates of microbial growth and respiration and are substantially different than those often assumed in the literature. These results suggest that without independent knowledge of these ratios, the uncertainty in tracer-derived microbial growth and respiration rates may be larger than previously reported. 66 refs., 8 figs., 3 tabs.

  10. Comprehensive assessment of toxic emissions from coal-fired power plants

    SciTech Connect (OSTI)

    1996-09-01

    The 1990 Clean Air Act Amendments (CAAA) have two primary goals: pollution prevention and a market-based least-cost approach to emission control. To address air quality issues as well as permitting and enforcement, the 1990 CAAA contain 11 sections or titles. The individual amendment titles are as follows: Title I - National Ambient Air Quality Standards Title II - Mobile Sources Title III - Hazardous Air Pollutants Title IV - Acid Deposition Control Title V - Permits Title VI - Stratospheric Ozone Protection Chemicals Title VII - Enforcement Title VIII - Miscellaneous Provisions Title IX - Clean Air Research Title X - Disadvantaged Business Concerns Title XI - Clean Air Employment Transition Assistance Titles I, III, IV, and V will change or have the potential to change how operators of coal-fired utility boilers control, monitor, and report emissions. For the purpose of this discussion, Title III is the primary focus.

  11. Development of protein based bioremediation and drugs for heavy metal toxicity

    SciTech Connect (OSTI)

    Opella, Stanley J.

    2001-09-18

    Structural studies were performed on several proteins of the bacterial detoxification system. These proteins are responsible for binding (MerP) and transport of heavy metals, including mercury, across membranes. The structural information obtained from NMR experiments provides insight into the selectivity and sequestration processes towards heavy metal toxins.

  12. A role for glutathione, independent of oxidative stress, in the developmental toxicity of methanol

    SciTech Connect (OSTI)

    Siu, Michelle T.; Shapiro, Aaron M.; Wiley, Michael J.; Wells, Peter G.

    2013-12-15

    Oxidative stress and reactive oxygen species (ROS) have been implicated in the teratogenicity of methanol (MeOH) in rodents, both in vivo and in embryo culture. We explored the ROS hypothesis further in vivo in pregnant C57BL/6J mice. Following maternal treatment with a teratogenic dose of MeOH, 4 g/kg via intraperitoneal (ip) injection on gestational day (GD) 12, there was no increase 6 h later in embryonic ROS formation, measured by 2?,7?-dichlorodihydrofluorescin diacetate (DCFH-DA) fluorescence, despite an increase observed with the positive control ethanol (EtOH), nor was there an increase in embryonic oxidatively damaged DNA, quantified as 8-oxo-2?-deoxyguanosine (8-oxodG) formation. MeOH teratogenicity (primarily ophthalmic anomalies, cleft palate) also was not altered by pre- and post-treatment with varying doses of the free radical spin trapping agent alpha-phenyl-N-tert-butylnitrone (PBN). In contrast, pretreatment with L-buthionine-(S,R)-sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, depleted maternal hepatic and embryonic GSH, and enhanced some new anomalies (micrognathia, agnathia, short snout, fused digits, cleft lip, low set ears), but not the most common teratogenic effects of MeOH (ophthalmic anomalies, cleft palate) in this strain. These results suggest that ROS did not contribute to the teratogenic effects of MeOH in this in vivo mouse model, in contrast to results in embryo culture from our laboratory, and that the protective effect of GSH in this model may arise from its role as a cofactor for formaldehyde dehydrogenase in the detoxification of formaldehyde. - Highlights: In vivo, a free radical scavenger did not block methanol (MeOH) teratogenesis. MeOH did not increase embryonic reactive oxygen species formation or DNA oxidation. MeOH teratogenesis was enhanced by glutathione (GSH) depletion. GSH may protect as the cofactor for formaldehyde dehydrogenase (ADH3). Formaldehyde may be a ROS-independent proximate teratogenic species in vivo.

  13. Emission factors for several toxic air pollutants from fluidized-bed combustion of coal

    SciTech Connect (OSTI)

    Smith, A.E.

    1986-03-01

    Clean coal technologies such as fluidized-bed combustion have the potential to emit the same trace elements as conventional combustors. Since the US Environmental Protection Agency (EPA) is likely to promulgate National Emission Standards for Hazardous Air Pollutants for several trace elements, the feasibility of using fluidized-bed combustors to reduce sulfur dioxide emissions may depend in part on the relative amounts of trace elements emitted by fluidized-bed and conventional combustors. Emissions of trace elements from both atmospheric and pressurized fluidized-bed combustors were compared with those from conventional combustors by developing fluidized-bed emission factors from information available in the literature and comparing them with the emission factors for conventional combustors recommended in a literature search conducted for EPA. The comparisons are based on the mass of emission per unit of heat input for antimony, arsenic, beryllium, cadmium, chromium, cobalt, copper, lead, manganese, mercury, nickel, vanadium, and zinc. When inaccuracies in the data were taken into account, the trace element emissions from atmospheric fluidized-bed combustion seem to be somewhat higher than those from a conventional utility boiler burning pulverized coal and somewhat lower than those from pressurized fluidized-bed combustion.

  14. Heterogeneous photocatalytic oxidation of toxic organic waste waters with supported semiconductor

    SciTech Connect (OSTI)

    Renzi, C.; Mazzarino, I.; Baldi, G.

    1996-12-31

    The photocatalytic oxidation of coumaric acid by dissolved oxygen in aqueous solution was carried using supported Titania catalysts. Thin catalytic layers on inert supports were obtained by Physical Vapor Deposition. The oxidation process was performed in two experimental reactors: a small-scale batch apparatus and a pilot-size continuous photocatalytic reactor (CPR). 6 refs., 6 figs., 1 tab.

  15. Cardiopulmonary Toxicity Induced by Ambient Particulate Matter (BI City Concentrated Ambient Particle Study)

    SciTech Connect (OSTI)

    Annette Rohr; James Wagner Masako Morishita; Gerald Keeler; Jack Harkema

    2010-06-30

    Alterations in heart rate variability (HRV) have been reported in rodents exposed to concentrated ambient particles (CAPs) from different regions of the United States. The goal of this study was to compare alterations in cardiac function induced by CAPs in two distinct regional atmospheres. AirCARE 1, a mobile laboratory with an EPA/Harvard fine particle (particulate matter <2.5 {micro}m; PM{sub 2.5}) concentrator was located in urban Detroit, MI, where the PM mixture is heavily influenced by motor vehicles, and in Steubenville, OH, where PM is derived primarily from long-range transport and transformation of power plant emissions, as well as from local industrial operations. Each city was studied during both winter and summer months, for a total of four sampling periods. Spontaneously hypertensive rats instrumented for electrocardiogram (ECG) telemetry were exposed to CAPs 8 h/day for 13 consecutive days during each sampling period. Heart rate (HR), and indices of HRV (standard deviation of the average normal-to-normal intervals [SDNN]; square root of the mean squared difference of successive normal-to-normal intervals [rMSSD]), were calculated for 30-minute intervals during exposures. A large suite of PM components, including nitrate, sulfate, elemental and organic carbon, and trace elements, were monitored in CAPs and ambient air. In addition, a unique sampler, the Semi-Continuous Elements in Air Sampler (SEAS) was employed to obtain every-30-minute measurements of trace elements. Positive matrix factorization (PMF) methods were applied to estimate source contributions to PM{sub 2.5}. Mixed modeling techniques were employed to determine associations between pollutants/CAPs components and HR and HRV metrics. Mean CAPs concentrations in Detroit were 518 and 357 {micro}g/m{sup 3} (summer and winter, respectively) and 487 and 252 {micro}g/m{sup 3} in Steubenville. In Detroit, significant reductions in SDNN were observed in the summer in association with cement/lime, iron/steel, and gasoline/diesel factors, while associations with the sludge incineration factor and components were less consistent. In winter, increases in HR were associated with a refinery factor and its components. CAPs-associated HR decreases in winter were linked to sludge incineration, cement/lime, and coal/secondary factors and the majority of their associated components. Specific relationships for increased rMSSD in winter were difficult to determine due to lack of consistency between factors and associated constituents. In Steubenville, we observed significant changes in HR (both increases and decreases), SDNN, and rMSSD in the summer, but not in the winter. We examined associations between individual source factors/PM components and HRV metrics segregated by predominant wind direction (NE or SW). Changes in HR (both increases and decreases) were linked with metal processing, waste incineration, and iron/steel factors along with most of their associated elemental constituents. Reductions in SDNN were associated with metal processing, waste incineration, and mobile source factors and the majority of elements loading onto these factors. There were no consistent associations between changes in rMSSD and source factors/components. Despite the large number of coal-fired power plants in the region, and therefore the large contribution of secondary sulfate to overall PM mass, we did not observe any associations with the coal/secondary factor or with the majority of its associated components. There were several inconsistencies in our results which make definitive conclusions difficult. For example, we observed opposing signs of effect estimates with some components depending on season, and with others depending on wind direction. In addition, our extensive dataset clearly would be subject to issues of multiple comparisons, and the 'true' significant results are unknown. Overall, however, our results suggest that acute changes in cardiac function were most strongly associated with local industrial sources. Results for coal-fired power plant-derived PM were

  16. Biochemical solubilization of toxic salts from residual geothermal brines and waste waters

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.S.

    1994-11-22

    A method of solubilizing metal salts such as metal sulfides in a geothermal sludge using mutant Thiobacilli selected for their ability to metabolize metal salts at high temperature is disclosed. The method includes the introduction of mutated Thiobacillus ferrooxidans and Thiobacillus thiooxidans to a geothermal sludge or brine. The microorganisms catalyze the solubilization of metal salts. For instance, in the case of metal sulfides, the microorganisms catalyze the solubilization to form soluble metal sulfates. 54 figs.

  17. Biochemical solubilization of toxic salts from residual geothermal brines and waste waters

    DOE Patents [OSTI]

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow S. (Rocky Point, NY)

    1994-11-22

    A method of solubilizing metal salts such as metal sulfides in a geothermal sludge using mutant Thiobacilli selected for their ability to metabolize metal salts at high temperature is disclosed, The method includes the introduction of mutated Thiobacillus ferrooxidans and Thiobacillus thiooxidans to a geothermal sludge or brine. The microorganisms catalyze the solubilization of metal salts, For instance, in the case of metal sulfides, the microorganisms catalyze the solubilization to form soluble metal sulfates.

  18. Integrated Microfluidics/Electrochemical Sensor System for Field-Monitoring of Toxic Metals

    SciTech Connect (OSTI)

    Lin, Yuehe; Matson, Dean W.; Bennett, Wendy D.; Thrall, K D.; Timchalk, Chuck; W. Ehrfeld

    2000-01-01

    Discusses a miniaturized analytical system based on a microfluidics/electrochemical detection scheme. Individual modules, such as microfabricated piezoelectrically actuated pumps, a micro-membrane separator and a microelectrochemical cell will be integrated onto a portable platform.

  19. Lung Toxicity and Mutagenicity of Emissions From Heavy-Duty Compressed...

    Broader source: Energy.gov (indexed) [DOE]

    5 Diesel Engine Emissions Reduction (DEER) Conference Presentations and Posters PDF icon 2005deermauderly.pdf More Documents & Publications Relationship Between Composition and ...

  20. A vast collection of microbial genes that are toxic to bacteria...

    Office of Scientific and Technical Information (OSTI)

    initiator dnaA. Our results show that cloning gaps, once considered the result of ... Resource Type: Journal Article Resource Relation: Journal Name: Genome Research; Journal ...

  1. Integrated chemical/biological treatment of paint stripper mixed waste: Metals toxicity and separation

    SciTech Connect (OSTI)

    Vanderberg-Twary, L.; Grumbine, R.K.; Foreman, T.; Hanners, J.L.; Brainard, J.R.; Sauer, N.N.; Unkefer, P.J.

    1995-05-01

    The DOE complex has generated vast quantities of complex heterogeneous mixed wastes. Paint stripper waste (PSW) is a complex waste that arose from decontamination and decommissioning activities. It contains paint stripper, cheesecloth, cellulose-based paints with Pb and Cr, and suspect Pu. Los Alamos National Laboratory has 150--200 barrels of PSW and other national laboratories such as Rocky Flats Plant have many more barrels of heterogeneous waste. Few technologies exist that can treat this complex waste. Our approach to solving this problem is the integration of two established technologies: biodegradation and metals chelation.

  2. Green alternatives to toxic release inventory (TRI) chemicals in the process industry

    SciTech Connect (OSTI)

    Ahmed, I.; Baron, J.; Hamilton, C.

    1995-12-01

    Driven by TRI reporting requirements, the chemical process industry is searching for innovative ways to reduce pollution at the source. Distinct environmental advantages of biobased green chemicals (biochemicals) mean are attractive alternatives to petrochemicals. Biochemicals are made from renewable raw materials in biological processes, such as aerobic and anaerobic fermentation, that operate at ambient temperatures and pressures, and produce only nontoxic waste products. Key TRI chemicals and several classes of commodity and intermediate compounds, used on consumer end-products manufacturing, are examined and alternatives are suggested. Specific substitution options for chlorofluorocarbons, industrial solvents, and commodity organic and inorganic chemicals are reviewed. Currently encouraged pollution prevention alternatives in the manufacturing sector are briefly examined for their long-term feasibility such as bioalternatives to bleaching in the pulp & paper industry, solvent cleaning in the electronics and dry cleaning industries, and using petroleum-based feedstocks in the plastics industry. Total life cycle and cost/benefit analyses are employed to determine whether biochemicals are environmentally feasible and commercially viable as pollution prevention tools. Currently available green chemicals along with present and projected costs and premiums are also presented. Functional compatibility of biochemicals with petrochemicals and bioprocessing systems with conventional chemical processing methods are explored. This review demonstrates that biochemicals can be used cost effectively in certain industrial chemical operations due to their added environmental benefits.

  3. Corporate planning for compliance with EPA`s proposed risk management program rule

    SciTech Connect (OSTI)

    Mannan, M.; Keeney, R.C.

    1995-12-31

    On October 20, 1993, the US Environmental Protection Agency (EPA) published in the Federal Register the proposed rule entitled Risk Management Programs for Chemical Accidental Release Prevention (40 CFR 68). Subsequently, on January 31, 1994, EPA published in the Federal Register the finalized list of 77 regulated toxic substances and 63 regulated flammable substances that are to be covered under the rule along with the associated threshold quantities for each substance. This list of substances will dictate which stationary sources will have to comply with the requirements of the proposed risk management program rule. The risk management program rule will most likely be finalized sometime after mid-1995. Covered facilities will then have a total of three years to achieve complete compliance with the requirements of the rule. This paper presents an approach for corporations with multiple sites to develop action plans for implementation of the risk management program rule. The process starts with a determination of which facilities are potentially covered, development of a matrix of requirements that each facility must comply with, and finally, common strategies that may be used in achieving compliance. Thus, a multi-facility corporation can develop a baseline compliance guideline document that individual plants can use in developing and implementing their risk management programs.

  4. Information resources: How they are utilized by Louisiana

    SciTech Connect (OSTI)

    Gardner, S.

    1990-12-31

    Louisiana, now in a developmental stage of policy and planning, has completed a project aimed at reducing hazardous releases of air toxics in thee state. The state is also conducting a Comparative Risk Project and is using risk assessment practices to develop its waste quality standards. In developing an air toxic list, Louisiana incorporated four major criteria into the ranking: emission levels, human health effects, potential population exposure, and persistence or accumulation in the environment. For the human health effects criterion, data for each substance was gathered from numerous sources, although the Integrated Risk Information System (IRIS) database was used as a primary source for toxicological information. Following guidelines established by the Environmental Protection Agency (EPA), the Office of Water Resources, Water Pollution Control Division, has developed numerical criteria for human health protection based on risk assessment procedures in the 1989 Water Quality Standards Revision. Currently over 30 toxic substances have risk-based criteria for th protection of human health in the standards. Numerical criteria were calculated for carcinogenic substances having an EPA Classification of A, B1, B2, or C. Cancer class designations along with cancer potency slopes and reference doses were extracted from the IRIS database, with the exception of those chemicals that had not been assessed in IRIS as of December 1, 1988. The parameters necessary for calculating human health criteria for the missing chemicals were taken from 1980, 1984, and 1985 ambient water quality criteria documents: data on bioconcentration factors were included. Currently, Louisiana is working on a Comparative Risk Project, a ranking of the environmental issues in the state relative to potential risk to the public, which is the basis for a widespread 1991 public outreach effort.

  5. Idaho CERCLA Disposal Facility Complex Compliance Demonstration for DOE Order 435.1

    SciTech Connect (OSTI)

    Simonds, J.

    2007-11-06

    This compliance demonstration document provides an analysis of the Idaho CERCLA Disposal Facility (ICDF) Complex compliance with DOE Order 435.1. The ICDF Complex includes the disposal facility (landfill), evaporation pond, administration facility, weigh scale, and various staging/storage areas. These facilities were designed and constructed to be compliant with DOE Order 435.1, Resource Conservation and Recovery act Subtitle C, and Toxic Substances Control Act polychlorinated biphenyl design and construction standards. The ICDF Complex is designated as the Idaho National Laboratory (INL) facility for the receipt, staging/storage, treatment, and disposal of INL Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) waste streams.

  6. Department of Energy reaches an agreement in principle on the cleanup of

    Energy Savers [EERE]

    the Santa Susana Field Lab site in Simi Valley | Department of Energy reaches an agreement in principle on the cleanup of the Santa Susana Field Lab site in Simi Valley Department of Energy reaches an agreement in principle on the cleanup of the Santa Susana Field Lab site in Simi Valley September 3, 2010 - 12:00am Addthis WASHINGTON, D.C. - The Department of Energy announced today that it has reached an agreement in principle with California's Department of Toxic Substances Control (DTSC)

  7. Ratiometric Sensing of Toxins using Quantum Dots | Argonne National

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory Ratiometric Sensing of Toxins using Quantum Dots December 4, 2015 Tweet EmailPrint Botulinum neurotoxin (BoNT) presents a significant hazard under numerous realistic scenarios. BoNT is the most toxic substance known. Current tests for BoNT are slow (2 days), lab-based, and complex. A new scheme offers quick, specific quantification by non-specialized personnel such as first responders in the field. The standard detection scheme for this fast-acting toxin is a lab-based mouse

  8. Microsoft Word - S06815_2010 Ann Rpt

    Office of Legacy Management (LM)

    6-1 6.0 References ATSDR (Agency for Toxic Substances and Disease Registry), 2004. Toxicological Profile for Strontium, U.S. Department Of Health And Human Services, Public Health Service, April. DOE (U.S. Department of Energy), 1994. Baseline Risk Assessment of Ground Water Contamination at the Uranium Mill Tailings Site at Shiprock, New Mexico, DOE/AL/62350-48F, Rev. 1, April. DOE (U.S. Department of Energy), 2000. Final Site Observational Work Plan for the Shiprock, New Mexico, UMTRA Project

  9. Project of the Month | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project of the Month Project of the Month RSS January 1, 2013 Members of the team that helped safely close the Toxic Substances Control Act Incinerator at Oak Ridge gather for a photo. The team is from URS|CH2M, Oak Ridge, the prime contractor for the Oak Ridge Office of Environmental Management. One-of-a-Kind Facility Now in Safe Shutdown OAK RIDGE, Tenn. - Oak Ridge's East Tennessee Technology Park (ETTP) is home to many unique facilities, each constructed for a specific purpose. Today, almost

  10. EO 12088: Federal Compliance with Pollution Control Standards

    Office of Environmental Management (EM)

    2088-Federal Compliance with Pollution Control Standards SOURCE: The provisions of Executive Order 12088 of October 13,1978, appear at 43 FR 47707, 3 CFR, 1978 Comp., p. 243, unless otherwise noted. By the authority vested in me as President by the Constitution and statutes of the United States of America, including Section 22 of the Toxic Substances Control Act (15 U.S.C. 2621), Section 313 of the Federal Water Pollution Control Act, as amended (33 U.S.C. 1323), Section 1447 of the Public

  11. Health assessment for Cedartown Municipal Landfill NPL Site, Cedartown, Polk County, Georgia, Region 4. CERCLIS No. GAD980495402. Preliminary report

    SciTech Connect (OSTI)

    Not Available

    1990-08-08

    In compliance with the Comprehensive Environmental Response, Compensation, and Liability Act and the Resource Conservation and Recovery Act, as amended, the Agency for Toxic Substances and Disease Registry (ATSDR) has prepared Health Assessment reports for sites currently on, or proposed for, the National Priorities List. In the report, the presence and nature of health hazards at this site are assessed, and the public health implications specific to this site are evaluated. The Health Assessment is based on such factors as the nature, concentration, toxicity, and extent of contamination at the site; the existence of potential pathways for the human exposure; the size and nature of the community likely to be exposed; and any other information available.

  12. Engineering test report: paint waste reduction fluidized-bed process demonstration at Letterkenny Army Depot Chambersburg, Pennsylvania. Final report, May 90-Jul 91

    SciTech Connect (OSTI)

    Murphy, J.P.; Parker, D.

    1991-07-01

    Degreasing and removal of paint from metal parts are processes performed at several Army depots across the country as part of vehicle and equipment rebuilding operations. These processes generate many tons of hazardous waste and release some hazardous materials into the workplace because most of them incorporate toxic chlorinated solvents or caustic soda. These substances also produce sludges that are classified as hazardous waste. U.S. Army Depot Support Command (DESCOM), as part of its hazardous waste minimization program, has established as a goal the elimination of hazardous waste generation from paint stripping operations. Through specific research and development projects, the U.S. Army's Toxic and Hazardous Materials Agency (USATHAMA) assists Army Depots in developing and evaluating methods for minimizing the quantities of hazardous wastes that they generate.

  13. Final Technical Report for project "Factors Controlling In Situ Uranium and Technetium Bio-Reduction and Reoxidation at the NABIR Field Research Center"

    SciTech Connect (OSTI)

    Dr. Jonathan D. Istok , Oregon State University; Dr. Lee Krumholz, University of Oklahoma; Dr. James McKinley, Pacific Northwest National Laboratory; Dr. Baohua Gu, Oak Ridge National Laboratory

    2006-10-31

    The overall goal of this project was to better understand factors and processes controlling microbially-mediated reduction and reoxidation of U and Tc in the unconsolidated residuum overlying the Nolichucky shale at the Field Research Center (FRC) at Oak Ridge National Laboratory. Project activities were designed to test the following hypotheses: 1. The small rates of denitrification and U bio-reduction observed in laboratory incubations of sediments from FRC Area 1 at low pH (< 5) are due to the presence of high concentrations of toxic metals (especially Al and Ni). Rates of Tc reduction will also be small at low pH in the presence of high concentrations of toxic metals. 2. In situ rates of U and perhaps Tc bio-reduction can be increased by increasing system pH and thus precipitating toxic metals from solution. 3. In situ rates of U and Tc bio-reduction can be increased by the addition of humic substances, which complex toxic metals such as Al and Ni, buffer pH, and serve as electron shuttles to facilitate U and Tc reduction. 4. Microbially-reduced U and Tc are rapidly oxidized in the presence of high concentrations of NO3- and the denitrification intermediates NO2-, N2O, and NO. 5. An electron-donor-addition strategy (type and form of donor, with or without pH adjustment and with or without the co-addition of humic substances) can be devised to reduce U and Tc concentrations for an extended period of time in low pH groundwater in the presence of high concentrations of NO3-, Al, and Ni. This strategy operates by removing or complexing these components of FRC groundwater to allow the subsequent reduction of U(VI) and Tc(VII).

  14. Hepatic injury induces contrasting response in liver and kidney to chemicals that are metabolically activated: Role of male sex hormone

    SciTech Connect (OSTI)

    Kim, Young C. [College of Pharmacy, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul (Korea, Republic of)], E-mail: youckim@snu.ac.kr; Yim, Hye K.; Jung, Young S. [College of Pharmacy, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul (Korea, Republic of); Park, Jae H. [College of Veterinary Medicine, Seoul National University, San 56-1 Shinrim-Dong, Kwanak-Ku, Seoul (Korea, Republic of); Kim, Sung Y. [College of Pharmacy, Wonkwang University, 344-2 Shinyong-Dong, Iksan (Korea, Republic of)

    2007-08-15

    Injury to liver, resulting in loss of its normal physiological/biochemical functions, may adversely affect a secondary organ. We examined the response of the liver and kidney to chemical substances that require metabolic activation for their toxicities in mice with a preceding liver injury. Carbon tetrachloride treatment 24 h prior to a challenging dose of carbon tetrachloride or acetaminophen decreased the resulting hepatotoxicity both in male and female mice as determined by histopathological examination and increases in serum enzyme activities. In contrast, the renal toxicity of the challenging toxicants was elevated markedly in male, but not in female mice. Partial hepatectomy also induced similar changes in the hepatotoxicity and nephrotoxicity of a challenging toxicant, suggesting that the contrasting response of male liver and kidney was associated with the reduction of the hepatic metabolizing capacity. Carbon tetrachloride pretreatment or partial hepatectomy decreased the hepatic xenobiotic-metabolizing enzyme activities in both sexes but elevated the renal p-nitrophenol hydroxylase, p-nitroanisole O-demethylase and aminopyrine N-demethylase activities significantly only in male mice. Increases in Cyp2e1 and Cyp2b expression were also evident in male kidney. Castration of males or testosterone administration to females diminished the sex-related differences in the renal response to an acute liver injury. The results indicate that reduction of the hepatic metabolizing capacity induced by liver injury may render secondary target organs susceptible to chemical substances activated in these organs. This effect may be sex-specific. It is also suggested that an integrated approach should be taken for proper assessment of chemical hazards.

  15. Comparison of AB2588 multipathway risk factors for California fossil-fuel power stations

    SciTech Connect (OSTI)

    Gratt, L.B.; Levin, L.

    1997-12-31

    Substances released from power plants may travel through various exposure pathways resulting in human health and environmental risks. The stack air emission`s primary pathway is inhalation from the ambient air. Multipathway factors (adjustment factors to the inhalation risk) are used to evaluate the importance of non-inhalation pathways (such as ingestion and dermal contact). The multipathway factor for a specific substance is the health risk by all pathways divided by the inhalation health risk for that substance. These factors are compared for fossil fuel power stations that submitted regulatory risk assessments in compliance with California Toxic Hot Spots Act (AB2588). Substances representing the largest contributions to the cancer risk are of primary concern: arsenic, beryllium, cadmium, chromium (+6), formaldehyde, nickel, lead, selenium, and PAHs. Comparisons of the chemical-specific multipathway factors show the impacts of regulatory policy decisions on the estimated health risk for trace substances. As an example, point estimates of the soil mixing depth, varying from 1 cm to 15 cm, relate to the relative importance of the pathway. For the deeper mixing depths, the root-zone uptake by homegrown tomato plants (for assumed consumption rate of 15% for San Diego) may result in high multipathway factors for several trace metals. For shallower mixing depths, soil ingestion may become the dominant non-inhalation pathway. These differences may lead to significantly different risk estimates for similar facilities located at different California locations such as to be under local regulatory authorities. The overall multipathway factor for the total cancer risk is about 2, much smaller than some of the chemical-specific factors. Science-based multipathway analysis should reduce much of the concern that may be due to policy-based decisions on pathway selection and high-value point-estimates of the parameters.

  16. In-situ remediation system for groundwater and soils

    DOE Patents [OSTI]

    Corey, J.C.; Kaback, D.S.; Looney, B.B.

    1993-11-23

    A method and system are presented for in-situ remediation of contaminated groundwater and soil where the contaminants, such as toxic metals, are carried in a subsurface plume. The method comprises selection and injection into the soil of a fluid that will cause the contaminants to form stable, non-toxic compounds either directly by combining with the contaminants or indirectly by creating conditions in the soil or changing the conditions of the soil so that the formation of stable, non-toxic compounds between the contaminants and existing substances in the soil are more favorable. In the case of non-toxic metal contaminants, sulfides or sulfates are injected so that metal sulfides or sulfates are formed. Alternatively, an inert gas may be injected to stimulate microorganisms in the soil to produce sulfides which, in turn, react with the metal contaminants. Preferably, two wells are used, one to inject the fluid and one to extract the unused portion of the fluid. The two wells work in combination to create a flow of the fluid across the plume to achieve better, more rapid mixing of the fluid and the contaminants. 4 figures.

  17. In-situ remediation system for groundwater and soils

    DOE Patents [OSTI]

    Corey, John C. (212 Lakeside Dr., Aiken, SC 29803); Kaback, Dawn S. (1932 Cottonwood Dr., Aiken, SC 29803); Looney, Brian B. (1135 Ridgemont Dr., Aiken, SC 29803)

    1993-01-01

    A method and system for in-situ remediation of contaminated groundwater and soil where the contaminants, such as toxic metals, are carried in a subsurface plume. The method comprises selection and injection into the soil of a fluid that will cause the contaminants to form stable, non-toxic compounds either directly by combining with the contaminants or indirectly by creating conditions in the soil or changing the conditions of the soil so that the formation of stable, non-toxic compounds between the contaminants and existing substances in the soil are more favorable. In the case of non-toxic metal contaminants, sulfides or sulfates are injected so that metal sulfides or sulfates are formed. Alternatively, an inert gas may be injected to stimulate microorganisms in the soil to produce sulfides which, in turn, react with the metal contaminants. Preferably, two wells are used, one to inject the fluid and one to extract the unused portion of the fluid. The two wells work in combination to create a flow of the fluid across the plume to achieve better, more rapid mixing of the fluid and the contaminants.

  18. Microsoft PowerPoint - 5 Richard Abitz

    Office of Environmental Management (EM)

    Performance Assessment of the Portsmouth On Site Waste Disposal Facility R.J. Abitz & J.D. Chiou Fluor-BWXT Portsmouth J. Reising DOE Portsmouth P&RA CoP Technical Exchange Meeting Richland, WA 16 December 2015 PORTS End-State Vision 2 Today Post Remediation OSWDF Overview 3 Key Features of OSWDF ~100-acre liner footprint with 10 cells and 2 contingent cells On competent bedrock and 100 feet above the upper most aquifer Over 5 million cubic yards of waste capacity Accept LLW, MLLW, TSCA,

  19. Approaches to the assessment of injuries to soil arising from discharges of hazardous substances and oil: Type B, Technical information document

    SciTech Connect (OSTI)

    Van Voris, P.; Dawson, G.W.; Fredrickson, J.K.; Cataldo, D.A.; Rogers, L.E.; Novich, C.M.; Meuser, J.

    1987-06-01

    Methods for determining the nature and magnitude of injury to the following natural resources are described for: soil chemical characteristics (acidity or pH, cation exchange capacity, percent base saturation, salinity); soil physical characteristics (porosity, water holding capacity, aggregate stability); biological characteristics (microbial activities, invertebrate activities, vegetation); and contaminant transport potential (leaching, food chain). In addition, this document explains how injuries to the soil resource can be translated into a reduction in service provided by that soil and how to determine soil recovery potential. That portion of 43 CFR Part 11 that pertains to the soils portion of the geologic resource is explained.

  20. Evaluation of AFBC co-firing of coal and hospital wastes. Technical report, January 1989--August 1990

    SciTech Connect (OSTI)

    Not Available

    1991-02-01

    The purpose of this program is to expand the use of coal by utilizing CFB (circulating fluidized bed) technology to provide an environmentally safe method for disposing of waste materials. Hospitals are currently experiencing a waste management crisis. In many instances, they are no longer permitted to burn pathological and infectious wastes in incinerators. Older hospital incinerators are not capable of maintaining the stable temperatures and residence times necessary in order to completely destroy toxic substances before release into the atmosphere. In addition, the number of available landfills which can safely handle these substances is decreasing each year. The purpose of this project is to conduct necessary research investigating whether the combustion of the hospital wastes in a coal-fired circulating fluidized bed boiler will effectively destroy dioxins and other hazardous substances before release into the atmosphere. If this is proven feasible, in light of the quantity of hospital wastes generated each year, it would create a new market for coal -- possibly 50 million tons/year.

  1. Evaluation of AFBC co-firing of coal and hospital wastes

    SciTech Connect (OSTI)

    Not Available

    1991-02-01

    The purpose of this program is to expand the use of coal by utilizing CFB (circulating fluidized bed) technology to provide an environmentally safe method for disposing of waste materials. Hospitals are currently experiencing a waste management crisis. In many instances, they are no longer permitted to burn pathological and infectious wastes in incinerators. Older hospital incinerators are not capable of maintaining the stable temperatures and residence times necessary in order to completely destroy toxic substances before release into the atmosphere. In addition, the number of available landfills which can safely handle these substances is decreasing each year. The purpose of this project is to conduct necessary research investigating whether the combustion of the hospital wastes in a coal-fired circulating fluidized bed boiler will effectively destroy dioxins and other hazardous substances before release into the atmosphere. If this is proven feasible, in light of the quantity of hospital wastes generated each year, it would create a new market for coal -- possibly 50 million tons/year.

  2. The use of field methods to evaluate the toxicity of lead to plants at a small arms firing range

    SciTech Connect (OSTI)

    DeShields, B.R.; Meredith, R.W.; Griffin, D.; Laughlin, T. [Harding Lawson Associates, Novato, CA (United States); Collins, W. [Army, Presidio of Monterey, CA (United States). Directorate of Environmental and Natural Resources Management

    1998-12-31

    The beach dunes at Fort Ord, California, were historically used as small arms firing ranges, resulting in the accumulation of spent bullets and varying concentrations of lead in soil. The form of the lead, and thus its bioavailability, is important in assessing associated ecological risks at firing ranges. Of particular interest at the beach firing ranges at Fort Ord are two species of buckwheat plants that provide habitat for an endangered butterfly. Initially, lead concentrations in soil and plant chaff were measured and root elongation bioassays were conducted. A linear correlation between lead in soil and lead in plant chaff was observed. However, the results of the bioassays were highly variable with no clear dose response pattern. Additional field studies were conducted to (1) further characterize lead concentrations in soil and plant tissue and (2) evaluate associations between soil lead concentrations and plant morphometric-variables. A relationship between soil and tissue lead concentrations was demonstrated. No significant associations between soil lead levels and plant health/condition were detected. Significant associations were observed between plant health/condition and factors other than lead.

  3. Green tea polyphenol, (?)-epigallocatechin-3-gallate, induces toxicity in human skin cancer cells by targeting ?-catenin signaling

    SciTech Connect (OSTI)

    Singh, Tripti; Katiyar, Santosh K.

    2013-12-01

    The green tea polyphenol, (?)-epigallocatechin-3-gallate (EGCG), has been shown to have anti-carcinogenic effects in several skin tumor models, and efforts are continued to investigate the molecular targets responsible for its cytotoxic effects to cancer cells. Our recent observation that ?-catenin is upregulated in skin tumors suggested the possibility that the anti-skin carcinogenic effects of EGCG are mediated, at least in part, through its effects on ?-catenin signaling. We have found that treatment of the A431 and SCC13 human skin cancer cell lines with EGCG resulted in reduced cell viability and increased cell death and that these cytotoxic effects were associated with inactivation of ?-catenin signaling. Evidence of EGCG-induced inactivation of ?-catenin included: (i) reduced accumulation of nuclear ?-catenin; (ii) enhanced levels of casein kinase1?, reduced phosphorylation of glycogen synthase kinase-3?, and increased phosphorylation of ?-catenin on critical serine{sup 45,33/37} residues; and (iii) reduced levels of matrix metalloproteinase (MMP)-2 and MMP-9, which are down-stream targets of ?-catenin. Treatment of cells with prostaglandin E2 (PGE{sub 2}) enhanced the accumulation of ?-catenin and enhanced ?-catenin signaling. Treatment with either EGCG or an EP2 antagonist (AH6809) reduced the PGE{sub 2}-enhanced levels of cAMP, an upstream regulator of ?-catenin. Inactivation of ?-catenin by EGCG resulted in suppression of cell survival signaling proteins. siRNA knockdown of ?-catenin in A431 and SCC13 cells reduced cell viability. Collectively, these data suggest that induction of cytotoxicity in skin cancer cells by EGCG is mediated by targeting of ?-catenin signaling and that the ?-catenin signaling is upregulated by inflammatory mediators. - Highlights: EGCG inhibits cancer cell viability through inactivation of ?-catenin signaling. Inactivation of ?-catenin involves the downregulation of inflammatory mediators. EGCG inactivates ?-catenin in skin cancer cells by inhibition of cAMP and PGE{sub 2}. siRNA knockdown of ?-catenin or COX-2 reduces the viability of cancer cells.

  4. Effect of palladium dispersion on the capture of toxic components from fuel gas by palladium-alumina sorbents

    SciTech Connect (OSTI)

    Baltrus, John P.; Granite, Evan J.; Rupp, Erik C.; Stanko, Dennis C.; Howard, Bret; Pennline, Henry W.

    2011-05-01

    The dispersion and location of Pd in alumina-supported sorbents prepared by different methods was found to influence the performance of the sorbents in the removal of mercury, arsine, and hydrogen selenide from a simulated fuel gas. When Pd is well dispersed in the pores of the support, contact interaction with the support is maximized, Pd is less susceptible to poisoning by sulfur, and the sorbent has better long-term activity for adsorption of arsine and hydrogen selenide, but poorer adsorption capacity for Hg. As the contact interaction between Pd and the support is lessened the Pd becomes more susceptible to poisoning by sulfur, resulting in higher capacity for Hg, but poorer long-term performance for adsorption of arsenic and selenium.

  5. Effect of palladium dispersion on the capture of toxic components from fuel gas by palladium-alumina sorbents

    SciTech Connect (OSTI)

    Baltrus, J.P.; Granite, E.J.; Rupp, E.C.; Stanko, D.C.; Howard, B.; Pennline, H.W.

    2011-01-01

    The dispersion and location of Pd in alumina-supported sorbents prepared by different methods was found to influence the performance of the sorbents in the removal of mercury, arsine, and hydrogen selenide from a simulated fuel gas. When Pd is well dispersed in the pores of the support, contact interaction with the support is maximized, Pd is less susceptible to poisoning by sulfur. and the sorbent has better long-term activity for adsorption of arsine and hydrogen selenide. but poorer adsorption capacity for Hg. As the contact interaction between Pd and the support is lessened the Pd becomes more susceptible to poisoning by sulfur. resulting in higher capacity for Hg, but poorer long-term performance for adsorption of arsenic and selenium.

  6. Imprint-coating synthesis of selective functionalized ordered mesoporous sorbents for separation and sensors

    DOE Patents [OSTI]

    Dai, Sheng (Knoxville, TN); Burleigh, Mark C. (Lenoir City, TN); Shin, Yongsoon (Richland, WA)

    2001-01-01

    The present invention relates generally to mesoporous sorbent materials having high capacity, high selectivity, fast kinetics, and molecular recognition capability. The invention also relates to a process for preparing these mesoporous substrates through molecular imprinting techniques which differ from convention techniques in that a template molecule is bound to one end of bifunctional ligands to form a complex prior to binding of the bifunctional ligands to the substrate. The present invention also relates to methods of using the mesoporous sorbent materials, for example, in the separation of toxic metals from process effluents, paints, and other samples; detection of target molecules, such as amino acids, drugs, herbicides, fertilizers, and TNT, in samples; separation and/or detection of substances using chromatography; imaging agents; sensors; coatings; and composites.

  7. Conceptual design report, Hazardous Materials Management and Emergency Response (HAMMER) Training Center

    SciTech Connect (OSTI)

    Kelly, K.E.

    1994-11-09

    For the next 30 years, the main activities at the US Department of Energy (DOE) Hanford Site will involve the management, handling, and cleanup of toxic substances. If the DOE is to meet its high standards of safety, the thousands of workers involved in these activities will need systematic training appropriate to their tasks and the risks associated with these tasks. Furthermore, emergency response for DOE shipments is the primary responsibility of state, tribal, and local governments. A collaborative training initiative with the DOE will strengthen emergency response at the Hanford Site and within the regional communities. Local and international labor has joined the Hazardous Materials Management and Emergency Response (HAMMER) partnership, and will share in the HAMMER Training Center core programs and facilities using their own specialized trainers and training programs. The HAMMER Training Center will provide a centralized regional site dedicated to the training of hazardous material, emergency response, and fire fighting personnel.

  8. Public health assessment for St. Louis Airport, Hazelwood Interim Storage/Futura Coatings Company, St. Louis, St. Louis County, Missouri, Region 7. Cerclis No. MOD980633176. Preliminary report

    SciTech Connect (OSTI)

    Not Available

    1994-01-20

    The St. Louis Airport/Hazelwood Iterim Storage/Futura Coatings Company, a National Priorities List site, is in St. Louis County, Missouri. From 1946 to 1973, the site was used to store radioactive materials resulting from uranium processing. High levels of uranium, thorium, radium, and radon were detected in soil, groundwater, and air. The site is still being used to store radioactive materials. The Agency for Toxic Substances and Disease Registry considers the St. Louis Airport site to be an indeterminate public health hazard. Although there are emissions of radon and the presence of thorium in on-site air and off-site soils and the emission of radiation resulting from the presence of these materials is not currently considered a health hazard. At present conditions, the concentration of radon off-site is indistinguishable from background levels. However, in the past, these contaminants may have been present at levels of health concern.

  9. 1998 Annual Site Environmental Report Tonopah Test Range, Nevada

    SciTech Connect (OSTI)

    Duncan, D.K.; Fink, C.H.; Sanchez, R.V.

    1999-09-01

    Sandia National Laboratories (SNL) operates the Tonopah Test Range (TTR) for the Department of Energy (DOE) Weapons Ordnance Program. This annual report (calendar year 1998) summarizes the compliance status to environmental regulations applicable at the site including those statutes that govern air and water quality, waste management cleanup of contaminated areas, control of toxic substances, and adherence to requirements as related to the National Environmental Policy Act (NEPA). In compliance with DOE orders, SNL also conducts environmental surveillance for radiological and nonradiological contaminants. SNL's responsibility for environmental surveillance at TTR extends only to those areas where SNL activities are carried out. Annual radiological and nonradiological routine releases and unplanned releases (occurrences) are also summarized. This report has been prepared in accordance with DOE Order 5400.1, General Environmental Protection Program (DOE 1990a).

  10. Project plan, Hazardous Materials Management and Emergency Response Training Center: Project 95L-EWT-100

    SciTech Connect (OSTI)

    Borgeson, M.E.

    1994-11-09

    The Hazardous Materials Management and Emergency Response (HAMMER) Training Center will provide for classroom lectures and hands-on practical training in realistic situations for workers and emergency responders who are tasked with handling and cleanup of toxic substances. The primary objective of the HAMMER project is to provide hands-on training and classroom facilities for hazardous material workers and emergency responders. This project will also contribute towards complying with the planning and training provisions of recent legislation. In March 1989 Title 29 Code of Federal Regulations Occupational Safety and Health Administration 1910 Rules and National Fire Protection Association Standard 472 defined professional requirements for responders to hazardous materials incidents. Two general types of training are addressed for hazardous materials: training for hazardous waste site workers and managers, and training for emergency response organizations.

  11. Chemicals identified in human biological media: a data base. Third annual report, October 1981

    SciTech Connect (OSTI)

    Cone, M.V.; Baldauf, M.F.; Martin, F.M.

    1981-12-01

    Part 2 contains the data base in tabular format. There are two sections, the first with records on nondrug substances, and the second with records on drugs. Chemicals in each section are arranged alphabetically by CAS preferred name, CAS registry number, formula, atomic weight, melting point, boiling point, and vapor pressure. Tissues are listed alphabetically with exposure route, analytical method, number of cases, range, and mean - when available in the source document. A variety of information may also be included that is pertinent to the range and mean as well as experimental design, demography, health effects, pathology, morphology, and toxicity. Review articles are included in the data base; however, no data have been extracted from such documents because the original research articles are included.

  12. 1997 annual site environmental report, Tonopah Test Range, Nevada

    SciTech Connect (OSTI)

    Culp, Todd; Duncan, Dianne; Forston, William; Sanchez, Rebecca

    1998-08-01

    Sandia National Laboratories (SNL) operates the Tonopah Test Range for the Department of Energy's (DOE) Weapons Ordnance Program. Thes annual report (calendar year 1997) summarizes the compliance status to environmental regulations applicable at the site including those statutes that govern air and water quality, waste management, cleanup of contaminated areas, control of toxic substances, and adherence to requirements as related to the National Environmental Policy Act. In compliance with DOE orders, SNL also conducts environmental surveillance for radiological and nonradiological contaminants. SNL's responsibility for environmental surveillance extends only to those activities performed by SNL or under its direction. Annual radiological and nonradiological routine releases and unplanned releases (occurrences) are also summarized. This report has been prepared as required by DOE Order 5400.1, General Environmental Protection Program.

  13. Risk-Based Disposal Plan for PCB Paint in the TRA Fluorinel Dissolution Process Mockup and Gamma Facilities Canal

    SciTech Connect (OSTI)

    R. A. Montgomery

    2008-05-01

    This Toxic Substances Control Act Risk-Based Polychlorinated Biphenyl Disposal plan was developed for the Test Reactor Area Fluorinel Dissolution Process Mockup and Gamma Facilities Waste System, located in Building TRA-641 at the Reactor Technology Complex, Idaho National Laboratory Site, to address painted surfaces in the empty canal under 40 CFR 761.62(c) for paint, and under 40 CFR 761.61(c) for PCBs that may have penetrated into the concrete. The canal walls and floor will be painted with two coats of contrasting non-PCB paint and labeled as PCB. The canal is covered with open decking; the access grate is locked shut and signed to indicate PCB contamination in the canal. Access to the canal will require facility manager permission. Protective equipment for personnel and equipment entering the canal will be required. Waste from the canal, generated during ultimate Decontamination and Decommissioning, shall be managed and disposed as PCB Bulk Product Waste.

  14. Characterization and validation of an in silico toxicology model to predict the mutagenic potential of drug impurities*

    SciTech Connect (OSTI)

    Valerio, Luis G.; Cross, Kevin P.

    2012-05-01

    Control and minimization of human exposure to potential genotoxic impurities found in drug substances and products is an important part of preclinical safety assessments of new drug products. The FDA's 2008 draft guidance on genotoxic and carcinogenic impurities in drug substances and products allows use of computational quantitative structureactivity relationships (QSAR) to identify structural alerts for known and expected impurities present at levels below qualified thresholds. This study provides the information necessary to establish the practical use of a new in silico toxicology model for predicting Salmonella t. mutagenicity (Ames assay outcome) of drug impurities and other chemicals. We describe the model's chemical content and toxicity fingerprint in terms of compound space, molecular and structural toxicophores, and have rigorously tested its predictive power using both cross-validation and external validation experiments, as well as case studies. Consistent with desired regulatory use, the model performs with high sensitivity (81%) and high negative predictivity (81%) based on external validation with 2368 compounds foreign to the model and having known mutagenicity. A database of drug impurities was created from proprietary FDA submissions and the public literature which found significant overlap between the structural features of drug impurities and training set chemicals in the QSAR model. Overall, the model's predictive performance was found to be acceptable for screening drug impurities for Salmonella mutagenicity. -- Highlights: ? We characterize a new in silico model to predict mutagenicity of drug impurities. ? The model predicts Salmonella mutagenicity and will be useful for safety assessment. ? We examine toxicity fingerprints and toxicophores of this Ames assay model. ? We compare these attributes to those found in drug impurities known to FDA/CDER. ? We validate the model and find it has a desired predictive performance.

  15. Laboratories for the 21st Century Best Practices: Energy Recovery in Laboratory Facilities

    SciTech Connect (OSTI)

    2012-06-01

    Laboratories typically require 100% outside air for ventilation at higher rates than other commercial buildings. Minimum ventilation is typically provided at air change per hour (ACH) rates in accordance with codes and adopted design standards including Occupational Safety and Health Administration (OSHA) Standard 1910.1450 (4 to 12 ACH non-mandatory) or the 2011 American Society of Heating, Refrigerating and Air Conditioning Engineers (ASHRAE) Applications Handbook, Chapter 16 Laboratories (6 to 12 ACH). While OSHA states this minimum ventilation rate should not be relied on for protection from toxic substances released into the laboratory it specifically indicates that it is intended to provide a source of air for breathing and for input to local ventilation devices (e.g., chemical fume hoods or exhausted bio-safety cabinets), to ensure that laboratory air is continually replaced preventing the increase of air concentrations of toxic substances during the working day, direct air flow into the laboratory from non-laboratory areas and out to the exterior of the building. The heating and cooling energy needed to condition and move this outside air can be 5 to 10 times greater than the amount of energy used in most office buildings. In addition, when the required ventilation rate exceeds the airflow needed to meet the cooling load in low-load laboratories, additional heating energy may be expended to reheat dehumidified supply air from the supply air condition to prevent over cooling. In addition to these low-load laboratories, reheat may also be required in adjacent spaces such as corridors that pro-vide makeup air to replace air being pulled into negative-pressure laboratories.

  16. Process safety management (OSHA) and process risk management (CAA) application. Application to a coke plant

    SciTech Connect (OSTI)

    Graeser, W.C.; Mentzer, W.P.

    1995-12-01

    Risk Management Programs for Chemical Accidental Release Prevention is the name of the proposed rule for the RMP Risk Management Program. The RMP was written in response to several catastrophic releases of hazardous substances. The rule is applicable to facilities that store, process or use greater than threshold quantities of 62 listed flammable chemicals and another 100 listed toxic substances. Additionally, a Risk Management Plan is registered with the EPA, Chemical Safety and Hazardous Investigation Board, state governments and the local emergency planning commission. The Clean Air Act Amendments of 1990 (specifically Section 112r) required the EPA to develop a three phase Risk Management Plan for industry: prevention program; hazard assessment; and emergency response program. The Prevention Program closely follows the OSHA`s Process Safety Management Standard. The Hazard Assessment section requires facilities to develop plans for a worst case scenario. The Emergency Response section defines the steps the facility and each employee will take if a release occurs. This section also needs to be coordinated with the Local Emergency Planning Commission. These regulations are described using Clairton Works as an example of compliance.

  17. An OSHA based approach to safety analysis for nonradiological hazardous materials

    SciTech Connect (OSTI)

    Yurconic, M.

    1992-08-01

    The PNL method for chemical hazard classification defines major hazards by means of a list of hazardous substances (or chemical groups) with associated trigger quantities. In addition, the functional characteristics of the facility being classified is also be factored into the classification. In this way, installations defined as major hazard will only be those which have the potential for causing very serious incidents both on and off site. Because of the diversity of operations involving chemicals, it may not be possible to restrict major hazard facilities to certain types of operations. However, this hazard classification method recognizes that in the industrial sector major hazards are most commonly associated with activities involving very large quantities of chemicals and inherently energetic processes. These include operations like petrochemical plants, chemical production, LPG storage, explosives manufacturing, and facilities which use chlorine, ammonia, or other highly toxic gases in bulk quantities. The basis for this methodology is derived from concepts used by OSHA in its proposed chemical process safety standard, the Dow Fire and Explosion Index Hazard Classification Guide, and the International Labor Office`s program on chemical safety. For the purpose of identifying major hazard facilities, this method uses two sorting criteria, (1) facility function and processes and (2) quantity of substances to identify facilities requiringclassification. Then, a measure of chemical energy potential (material factor) is used to identify high hazard class facilities.

  18. An OSHA based approach to safety analysis for nonradiological hazardous materials

    SciTech Connect (OSTI)

    Yurconic, M.

    1992-08-01

    The PNL method for chemical hazard classification defines major hazards by means of a list of hazardous substances (or chemical groups) with associated trigger quantities. In addition, the functional characteristics of the facility being classified is also be factored into the classification. In this way, installations defined as major hazard will only be those which have the potential for causing very serious incidents both on and off site. Because of the diversity of operations involving chemicals, it may not be possible to restrict major hazard facilities to certain types of operations. However, this hazard classification method recognizes that in the industrial sector major hazards are most commonly associated with activities involving very large quantities of chemicals and inherently energetic processes. These include operations like petrochemical plants, chemical production, LPG storage, explosives manufacturing, and facilities which use chlorine, ammonia, or other highly toxic gases in bulk quantities. The basis for this methodology is derived from concepts used by OSHA in its proposed chemical process safety standard, the Dow Fire and Explosion Index Hazard Classification Guide, and the International Labor Office's program on chemical safety. For the purpose of identifying major hazard facilities, this method uses two sorting criteria, (1) facility function and processes and (2) quantity of substances to identify facilities requiringclassification. Then, a measure of chemical energy potential (material factor) is used to identify high hazard class facilities.

  19. Contamination source review for Building E7995, Edgewood Area, Aberdeen Proving Ground, Maryland

    SciTech Connect (OSTI)

    Booher, M.N.; Miller, G.A.; Draugelis, A.K.; Glennon, M.A.; Rueda, J.; Zimmerman, R.E.

    1995-09-01

    The US Army Aberdeen Proving Ground (APG) commissioned Argonne National Laboratory (ANL) to conduct a contamination source review to identify and define areas of toxic or hazardous contaminants and to assess the physical condition and accessibility of APG buildings. The information obtained from the review may be used to assist the US Army in planning for the future use or disposition, of the buildings. The source contamination review consisted of the following tasks: historical records search, physical inspection, photographic documentation, geophysical investigation, investigation of potential hazardous materials facilities (HMFs), and review of available records regarding underground storage tanks. This report provides the results of the contamination source review for Building E7995. any of the APG facilities constructed between 1917 and the 1960s are no longer used because of obsolescence and their poor state of repair. Because many of these buildings were used for research, development, testing, and/or pilot-scale production of chemical warfare agents and other military substances, the potential exists for portions of the buildings to be contaminated with these substances, their degradation products, and other laboratory or industrial chemicals. These buildings, and associated structures or appurtenances, may contribute to environmental concerns at APG.

  20. Determination of a mutational spectrum

    DOE Patents [OSTI]

    Thilly, William G. (Winchester, MA); Keohavong, Phouthone (Cambridge, MA)

    1991-01-01

    A method of resolving (physically separating) mutant DNA from nonmutant DNA and a method of defining or establishing a mutational spectrum or profile of alterations present in nucleic acid sequences from a sample to be analyzed, such as a tissue or body fluid. The present method is based on the fact that it is possible, through the use of DGGE, to separate nucleic acid sequences which differ by only a single base change and on the ability to detect the separate mutant molecules. The present invention, in another aspect, relates to a method for determining a mutational spectrum in a DNA sequence of interest present in a population of cells. The method of the present invention is useful as a diagnostic or analytical tool in forensic science in assessing environmental and/or occupational exposures to potentially genetically toxic materials (also referred to as potential mutagens); in biotechnology, particularly in the study of the relationship between the amino acid sequence of enzymes and other biologically-active proteins or protein-containing substances and their respective functions; and in determining the effects of drugs, cosmetics and other chemicals for which toxicity data must be obtained.

  1. VAC*TRAX vacuum thermal desorption

    SciTech Connect (OSTI)

    1994-09-01

    Pilot VAC*TRAX treatability tests were conducted on RCRA, TSCA, and RCRA/radioactive mixed wastes, to determine the efficiency in remediating organics` contaminated solids. The process volatilizes organic compounds by indirectly heating the feed material in a vacuum batch dryer and condensing the organics separately from the remaining solids. Contaminants included tetrachloroethene, bis(2-ethylhexyl)phthalate, pentachlorophenol, and PCBs. Treatment specifications were met: a tetrachloroethene removal >99.99% and PCB removal from a starting level of 990 ppM to a final level of <1 ppM. One test run was spiked with MoO{sub 3}, as a uranium simulant; the Mo remained in the treated solids, not transferring to the condensate. In the mixed waste tests, uranium present in a feed soil remained in the soil. Economic viability was demonstrated by achieving excellent treatment on a routine basis with both 4 and 6 hour heating cycles.

  2. Innovative Vitrification for Soil Remediation

    SciTech Connect (OSTI)

    Hnat, James G.; Patten, John S.; Jetta, Norman W.

    1996-12-31

    Vortec has successfully completed Phases 1 and 2 of a technology demonstration program for an ''Innovative Fossil Fuel Fired Vitrification Technology for Soil Remediation.'' The principal objective of the program is to demonstrate the ability of a Vortec Cyclone Melting System (CMS) to remediate DOE contaminated soils and other waste forms containing TM RCRA hazardous materials, low levels of radionuclides and TSCA (PCB) containing wastes. The demonstration program will verify the ability of this vitrification process to produce a chemically stable glass final waste form which passes both TCLP and PCT quality control requirements, while meeting all federal and state emission control regulations. The demonstration system is designed to process 36 ton/day of as-received drummed or bulk wastes. The processing capacity equates to approximately 160 barrels/day of waste materials containing 30% moisture at an average weight of 450 lbs./barrel.

  3. ORISE: Agents of Opportunity for Terrorism Continuing Medical...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Agents of Opportunity for Terrorism On-line CME Course on Toxic Radiological Materials, Toxic Industrial Chemicals, and Toxic Industrial Materials Dates Scheduled Available 247...

  4. Applicability of a field-portable toxic heavy metal detector, using a radioisotope-tagged metalloprotein, to DOE environmental remediation and waste minimization initiatives

    SciTech Connect (OSTI)

    Randles, K.E.; Bragg, D.J.; Bodette, D.E.; Lipinski, R.J.; Luera, T.F.

    1998-08-01

    A system based on the metal-binding kidney protein, metallothionein, bound with a trace quantity of radioactive metal, has been shown to be capable of detecting parts-per-million (ppm) to parts-per-billion (ppb) concentrations of some heavy metals in liquid solution. The main objective of this study was to determine if this type of system has adequate sensitivity and selectivity for application in detecting a number of metallic species of concern to DOE, such as mercury, lead, and chromium. An affinity-displacement study is reported here using the heavy metal radiotracers {sup 65}Zn and {sup 109}Cd bound to metallothionein immobilized on an Affi-Gel 10 filter support. When a heavy metal solution with a greater affinity than the tracer for the protein is poured through the filter the radiotracer is displaced by a mechanism similar to ion exchange. The main objective of this study was to verify previous internal experimental parameters and results, and to determine the specific affinities of metallothionein for the metallic species of most concern to DOE.

  5. A process for containment removal and waste volume reduction to remediate groundwater containing certain radionuclides, toxic metals and organics. Final report

    SciTech Connect (OSTI)

    Buckley, L.P.; Killey, D.R.W.; Vijayan, S.; Wong, P.C.F.

    1992-09-01

    A project to remove groundwater contaminants by an improved treatment process was performed during 1990 October--1992 March by Atomic Energy of Canada Limited for the United States Department of Energy, managed by Argonne National Laboratory. The goal was to generate high-quality effluent while minimizing secondary waste volume. Two effluent target levels, within an order of magnitude, or less than the US Drinking Water Limit, were set to judge the process effectiveness. The program employed mixed waste feeds containing cadmium, uranium, lead, iron, calcium, strontium-85-90, cesium-137, benzene and trichlorethylene in simulated and actual groundwater and soil leachate solutions. A combination of process steps consisting of sequential chemical conditioning, cross-flow microfiltration and dewatering by low temperature-evaporation, or filter pressing were effective for the treatment of mixed waste having diverse physico-chemical properties. A simplified single-stage version of the process was implemented to treat ground and surface waters contaminated with strontium-90 at the Chalk River Laboratories site. Effluent targets and project goals were met successfully.

  6. RCRA Subtitle C TSD facilities and solvent recovery facilities: Section 313 of the Emergency Planning and Community Right-to-Know Act. Toxic chemical release inventory; Industry guidance

    SciTech Connect (OSTI)

    1999-01-01

    The purpose of this guidance document is to assist facilities in SIC code 4953 that are regulated under the Resource Conservation and Recovery Act (RCRA), Subtitle C and facilities in SIC code 7389 that are primarily engaged in solvent recovery services on a contract or fee basis. This document explains the EPCRA Section 313 and PPA Section 6607 reporting requirements (collectively referred to as the EPCRA Section 313) reporting requirements, and discusses specific release and other waste management activities encountered at many facilities in these industries. The objectives of this manual are to: clarify EPCRA Section 313 requirements for industry; increase the accuracy and completeness of the data being reported by RCRA Subtitle C TSD and solvent recovery facilities; and reduce the level of effort expended by those facilities that prepare an EPCRA Section 313 report.

  7. Improved Meteorological Input for Atmospheric Release Decision support Systems and an Integrated LES Modeling System for Atmospheric Dispersion of Toxic Agents: Homeland Security Applications

    SciTech Connect (OSTI)

    Arnold, E; Simpson, M; Larsen, S; Gash, J; Aluzzi, F; Lundquist, J; Sugiyama, G

    2010-04-26

    When hazardous material is accidently or intentionally released into the atmosphere, emergency response organizations look to decision support systems (DSSs) to translate contaminant information provided by atmospheric models into effective decisions to protect the public and emergency responders and to mitigate subsequent consequences. The Department of Homeland Security (DHS)-led Interagency Modeling and Atmospheric Assessment Center (IMAAC) is one of the primary DSSs utilized by emergency management organizations. IMAAC is responsible for providing 'a single piont for the coordination and dissemination of Federal dispersion modeling and hazard prediction products that represent the Federal position' during actual or potential incidents under the National Response Plan. The Department of Energy's (DOE) National Atmospheric Release Advisory Center (NARAC), locatec at the Lawrence Livermore National Laboratory (LLNL), serves as the primary operations center of the IMAAC. A key component of atmospheric release decision support systems is meteorological information - models and data of winds, turbulence, and other atmospheric boundary-layer parameters. The accuracy of contaminant predictions is strongly dependent on the quality of this information. Therefore, the effectiveness of DSSs can be enhanced by improving the meteorological options available to drive atmospheric transport and fate models. The overall goal of this project was to develop and evaluate new meteorological modeling capabilities for DSSs based on the use of NASA Earth-science data sets in order to enhance the atmospheric-hazard information provided to emergency managers and responders. The final report describes the LLNL contributions to this multi-institutional effort. LLNL developed an approach to utilize NCAR meteorological predictions using NASA MODIS data for the New York City (NYC) region and demonstrated the potential impact of the use of different data sources and data parameterizations on IMAAC/NARAC fate and transport predictions. A case study involving coastal sea breeze circulation patterns in the NYC region was used to investigate the sensitivity of atmospheric dispersion results on the source of three-dimensional wind field data.

  8. Mixed ligand chelate therapy for plutonium and toxic metals from energy power production. Final report, April 15, 1977-October 14, 1980. [Mice

    SciTech Connect (OSTI)

    Schubert, J.

    1980-10-14

    The results of experiments are summarized on the ability of combinations of chelating agents to modify the genotoxicity or tissue distributions. The mutagenicities of Cr and of chelating agents were determined. The metals described in the report are Pu(IV), Cd(II), Cr(III), and Cr(VI). Accurate measurements were made of the ability of CaNa/sub 2/EDTA, CaNa/sub 3/DTPA, and DMPS to reduce mortality in mice given doses (i.p.) of CdCl/sub 2/ well above the 100% lethal level. The efficacy in terms of the mmoles/kg needed to reduce the mortality was: DTPA > EDTA > DMPS. The combination of DTPA + DMPS proved most promising though little evidence for mixed complex formation was noted. Potentiometric titration studies the case of Pu(IV) a few combinations proved effective, but only when given shortly after Pu administration and then only in the liver but not the skeleton. It is recommended that metabolically stable chelating agents be used in combinations, especially for those combinations which may form very stable mixed ligand chelates.

  9. 2009 Toxic Chemical Release Inventory Report for the Emergency Planning and Community Right-to-Know Act of 1986, Title III, Section 313

    SciTech Connect (OSTI)

    Environmental Stewardship Group

    2010-11-01

    For reporting year 2009, Los Alamos National Laboratory (LANL) submitted a Form R report for lead as required under the Emergency Planning and Community Right-to- Know Act (EPCRA) Section 313. No other EPCRA Section 313 chemicals were used in 2009 above the reportable thresholds. This document was prepared to provide a description of the evaluation of EPCRA Section 313 chemical use and threshold determinations for LANL for calendar year 2009, as well as to provide background information about data included on the Form R reports.

  10. Mangiferin exerts hepatoprotective activity against D-galactosamine induced acute toxicity and oxidative/nitrosative stress via Nrf2NF?B pathways

    SciTech Connect (OSTI)

    Das, Joydeep; Ghosh, Jyotirmoy; Roy, Anandita; Sil, Parames C.

    2012-04-01

    Mangiferin, a xanthone glucoside, is well known to exhibit antioxidant, antiviral, antitumor, anti-inflammatory and gene-regulatory effects. In the present study, we isolated mangiferin from the bark of Mangifera indica and assessed its beneficial role in galactosamine (GAL) induced hepatic pathophysiology. GAL (400 mg/kg body weight) exposed hepatotoxic rats showed elevation in the activities of serum ALP, ALT, levels of triglycerides, total cholesterol, lipid-peroxidation and reduction in the levels of serum total proteins, albumin and cellular GSH. Besides, GAL exposure (5 mM) in hepatocytes induced apoptosis and necrosis, increased ROS and NO production. Signal transduction studies showed that GAL exposure significantly increased the nuclear translocation of NF?B and elevated iNOS protein expression. The same exposure also elevated TNF-?, IFN-?, IL-1?, IL-6, IL-12, IL-18 and decreased IL-10 mRNA expressions. Furthermore, GAL also decreased the protein expression of Nrf2, NADPH:quinine oxidoreductase-1, heme oxygenase-1 and GST?. However, mangiferin administration in GAL intoxicated rats or coincubation of hepatocytes with mangiferin significantly altered all these GAL-induced adverse effects. In conclusion, the hepatoprotective role of mangiferin was due to induction of antioxidant defense via the Nrf2 pathway and reduction of inflammation via NF?B inhibition. Highlights: ?Galactosamine induces hepatocytes death via oxidative and nitrosative stress. ?Mangiferin exerts hepatoprotective effect/antioxidant defense via Nrf2 pathway. ?Mangiferin exerts anti-inflammatory responses by inhibiting NF-?B. ?Mangiferin suppresses galactosamine-induced repression of IL-10 mRNA.

  11. Revised Emergency Planning and Community Right-to-Know Act (EPCRA), Section 313, Toxic Chemical Release reporting for calendar year 1998

    SciTech Connect (OSTI)

    Evans, R.A.

    2000-01-01

    This report contains forms which contain information on the physical location of the Y-12 Plant and the amount of lead that was released to the East Fork Poplar Creek and amounts transferred to landfills on-site as well as landfills in Texas and South Carolina. Amounts are given in pounds per year.

  12. Using a flame ionization detector (FID) to continuously measure toxic organic vapors in a paint spray booth. Rept. for Jul 91-Jan 92

    SciTech Connect (OSTI)

    Whitfield, J.K.; Howe, G.B.; Pate, B.A.; Wander, J.D.

    1992-01-01

    The paper reports the demonstration of linear and similar responses of a Ratfisch RS-55CA flame ionization detector (FID) to a solvent mixture identical to the volatile organic compounds (VOCs) in the coating and catalyst (NSN 8010-01-336-3036) and to the calibrating gas (propane) used in field calibrations of the FID. Sensitivity and linearity have been shown to extend from 715 to 45 mg/cu m, which brackets the calculated short-term exposure limit (STEL) and lower action thresholds. Monitoring is maintained constantly and, under field conditions, equilibration occurs rapidly (analysis and output transpire in milliseconds). As a trigger for fail-safe conversion from recirculation mode to a straight-through paint spray booth configuration, the FID may confidently be expected to initiate a corrective response before a transient elevation of VOC concentrations overexposes area personnel.

  13. Focused feasibility study for surface soil at the main pits and pushout area, J-field toxic burning pits area, Aberdeen Proving Ground, Maryland

    SciTech Connect (OSTI)

    Patton, T.; Benioff, P.; Biang, C.; Butler, J.

    1996-06-01

    The Environmental Management Division of Aberdeen Proving Ground (APG), Maryland, is conducting a remedial investigation and feasibility study of the J-Field area at APG pursuant to the Comprehensive Environmental Response, Compensation, and Liability Act, as amended (CERCLA). J-Field is located within the Edgewood Area of APG in Harford County, Maryland. Since World War II, activities in the Edgewood Area have included the development, manufacture, testing, and destruction of chemical agents and munitions. These materials were destroyed at J-Field by open burning/open detonation. Portions of J-Field continue to be used for the detonation and disposal of unexploded ordnance (UXO) by open burning/open detonation under authority of the Resource Conservation and Recovery Act.

  14. Chromosome Damage and Cell Proliferation Rates in In Vitro Irradiated Whole Blood as Markers of Late Radiation Toxicity After Radiation Therapy to the Prostate

    SciTech Connect (OSTI)

    Beaton, Lindsay A.; Ferrarotto, Catherine; Marro, Leonora; Samiee, Sara; Malone, Shawn; Grimes, Scott; Malone, Kyle; Wilkins, Ruth C.

    2013-04-01

    Purpose: In vitro irradiated blood samples from prostate cancer patients showing late normal tissue damage were examined for lymphocyte response by measuring chromosomal aberrations and proliferation rate. Methods and Materials: Patients were selected from a randomized trial evaluating the optimal timing of dose-escalated radiation and short-course androgen deprivation therapy. Of 438 patients, 3% experienced grade 3 late radiation proctitis and were considered to be radiosensitive. Blood samples were taken from 10 of these patients along with 20 matched samples from patients with grade 0 proctitis. The samples were irradiated at 6 Gy and, along with control samples, were analyzed for dicentric chromosomes and excess fragments per cell. Cells in first and second metaphase were also enumerated to determine the lymphocyte proliferation rate. Results: At 6 Gy, there were statistically significant differences between the radiosensitive and control cohorts for 3 endpoints: the mean number of dicentric chromosomes per cell (3.26 0.31, 2.91 0.32; P=.0258), the mean number of excess fragments per cell (2.27 0.23, 1.43 0.37; P<.0001), and the proportion of cells in second metaphase (0.27 0.10, 0.46 0.09; P=.0007). Conclusions: These results may be a valuable indicator for identifying radiosensitive patients and for tailoring radiation therapy.

  15. Letter: Recent reports on television and in news papers indicate people are dying from exposures to toxic chemicals, nuclear power plant disasters, drunken drivers, and incompetent health care.

    Office of Legacy Management (LM)

  16. Absorption-emission optrode and methods of use thereof

    DOE Patents [OSTI]

    Hirschfeld, T.B.

    1990-05-29

    A method and apparatus are described for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance. 4 figs.

  17. Absorption-emission optrode and methods of use thereof

    DOE Patents [OSTI]

    Hirschfeld, Tomas B. (Livermore, CA)

    1990-01-01

    A method and apparatus for monitoring the physical and chemical properties of a sample fluid by measuring an optical signal generated by a fluorescent substance and modulated by an absorber substance. The emission band of the fluorescent substance overlaps the absorption band of the absorber substance, and the degree of overlap is dependent on the physical and chemical properties of the sample fluid. The fluorescent substance and absorber substance are immobilized on a substrate so that an effective number of molecules thereof are sufficiently close for resonant energy transfer to occur, thereby providing highly efficient modulation of the fluorescent emissions of the fluorescent substance by the absorber substance.

  18. DOE-STD-1120-2005; Integration of Environment, Safety, and Health...

    Office of Environmental Management (EM)

    ... HAZARD TYPE Hazardous Substances Physical Chemicals Metals Asbestos Radiological ... Identification Environmental Protection Facility Disposition Management ...

  19. Process for determining the polarity of a crude oil

    SciTech Connect (OSTI)

    de Andrade Bruning, I.M.R.

    1989-12-26

    This patent describes a process for determining the relative polarity of a crude oil or fraction thereof. It comprises: contacting a known test substance with a stationary phase of the crude oil or fraction in a gas chromatography column, and measuring the interaction between the test substance and the oil; contacting the known test substance with a stationary phase of a non-polar second substance in a gas chromatography column and measuring the interaction between the test substance and the non-polar second substance; and determining the polarity of the crude oil relative to the non-polar second substance from the measurements obtained.

  20. Self-referencing spectrophotometric measurements

    DOE Patents [OSTI]

    O'Rourke, Patrick E. (Martinez, GA); Van Hare, David R. (Aiken, SC)

    1994-01-01

    A method for measuring the concentration of a chemical substance by spectrophotometry comprising the steps of placing a sample of a photoreactive substance between the light source and a spectrophotometer, obtaining an absorption spectrum of the substance using a fixed amount of light from the light source, obtaining a second absorption spectrum after a short interval, comparing the two to determine the concentration of the chemical substance from the difference in the spectra. If the chemical substance is not photoreactive, a photoreactive mixture can be made with a photoreactive dye that has photoreactive properties unique to the mixture. Alternatively, an optically transparent substrate can absorb the substance or the dye/substance mixture.

  1. OSHA's approach to risk assessment for setting a revised occupational exposure standard for 1,3-butadiene

    SciTech Connect (OSTI)

    Grossman, E.A.; Martonik, J. )

    1990-06-01

    In its 1980 benzene decision (Industrial Union Department, ALF-CIO v. American Petroleum Institute, 448 U.S. 607 (1980)), the Supreme Court ruled that before he can promulgate any permanent health or safety standard, the Secretary (of Labor) is required to make a threshold finding that a place of employment is unsafe--in the sense that significant risks are present and can be lessened by a change in practices (448 U.S. at 642). The Occupational Safety and Health Administration (OSHA) has interpreted this to mean that whenever possible, it must quantify the risk associated with occupational exposure to a toxic substance at the current permissible exposure limit (PEL). If OSHA determines that there is significant risk to workers' health at its current standard, then it must quantify the risk associated with a variety of alternative standards to determine at what level, if any, occupational exposure to a substance no longer poses a significant risk. For rulemaking on occupational exposure to 1,3-butadiene, there are two studies that are suitable for quantitative risk assessment. One is a mouse inhalation bioassay conducted by the National Toxicology Program (NTP), and the other is a rat inhalation bioassay conducted by Hazelton Laboratories Europe. Of the four risk assessments that have been submitted to OSHA, all four have used the mouse and/or rat data with a variety of models to quantify the risk associated with occupational exposure to 1,3-butadiene. In addition, OSHA has performed its own risk assessment using the female mouse and female rat data and the one-hit and multistage models.

  2. Lessons learned from the EG&G consolidated hazardous waste subcontract and ESH&Q liability assessment process

    SciTech Connect (OSTI)

    Fix, N.J.

    1995-03-01

    Hazardous waste transportation, treatment, recycling, and disposal contracts were first consolidated at the Idaho National Engineering Laboratory in 1992 by EG&G Idaho, Inc. At that time, disposition of Resource, Conservation and Recovery Act hazardous waste, Toxic Substance Control Act waste, Comprehensive Environmental Response, Compensation, and Liability Act hazardous substances and contaminated media, and recyclable hazardous materials was consolidated under five subcontracts. The wastes were generated by five different INEL M&O contractors, under the direction of three different Department of Energy field offices. The consolidated contract reduced the number of facilities handling INEL waste from 27 to 8 qualified treatment, storage, and disposal facilities, with brokers specifically prohibited. This reduced associated transportation costs, amount and cost of contractual paperwork, and environmental liability exposure. EG&G reviewed this approach and proposed a consolidated hazardous waste subcontract be formed for the major EG&G managed DOE sites: INEL, Mound, Rocky Flats, Nevada Test Site, and 10 satellite facilities. After obtaining concurrence from DOE Headquarters, this effort began in March 1992 and was completed with the award of two master task subcontracts in October and November 1993. In addition, the effort included a team to evaluate the apparent awardee`s facilities for environment, safety, health, and quality (ESH&Q) and financial liability status. This report documents the evaluation of the process used to prepare, bid, and award the EG&G consolidated hazardous waste transportation, treatment, recycling, and/or disposal subcontracts and associated ESH&Q and financial liability assessments; document the strengths and weaknesses of the process; and propose improvements that would expedite and enhance the process for other DOE installations that used the process and for the re-bid of the consolidated subcontract, scheduled for 1997.

  3. mu-Scale Variations Of Elemental Composition In Individual Atmospheric Particles By Means Of Synchrotron Radiation Based mu-XRF Analysis

    SciTech Connect (OSTI)

    Schleicher, N.; Kramar, U.; Norra, S.; Dietze, V.; Kaminski, U.; Cen, K.; Yu, Y.

    2010-04-06

    Atmospheric pollution poses a huge challenge especially for densely populated urban areas. Although a tremendous knowledge already exists on atmospheric particulate pollution, only very limited knowledge is available on mineral and chemical composition of single atmospheric particles because most studies on air pollution focus on total mass concentrations or bulk elemental analysis. However, it is of particular importance to investigate the properties of single particles since according to their individually composition they differ in their specific impact on climate change, negative environment and health effects, as well as accelerating the weathering of stone buildings in urban areas. Particles with sulfate and nitrate coatings together with sufficient moisture increase metal solubility and possibly catalyze further surface reactions on stone facades of buildings. From the viewpoint of health effects of aerosols it is important to consider agglomerations processes of fine anthropogenic and highly toxic particles with coarse geogenic and less toxic particles. With respect to fundamental research in mineralogy, processes forming composed coarse particles consisting of geogenic and anthropogenic substances are valuable to study since a new type of particle is produced. In this context, the important and still in detail unknown role of geogenic particles as catchers for anthropogenic aerosols can be investigated more closely. Coarse particles can provide a possible sink for fine particles. Moreover, the intermixture of particles from geogenic and anthropogenic sources and the spatial and temporal variations of contributions from different sources, which plays a decisive role in the study area of Beijing, can be clarified with this approach. For this study, particles were collected with the passive sampling device Sigma-2 and analyzed for particles from 3 to 96 {mu}m. The analyzed particles showed a very inhomogeneous distribution in their elemental composition. For this study, synchrotron radiation based mu-X-ray fluorescence analysis (mu-SXRF) proved to be an excellent tool to investigate mu-scalic distributions of main and trace element concentrations within individual airborne particles.

  4. COMPARISON OF CLEAN DIESEL BUSES TO CNG BUSES

    SciTech Connect (OSTI)

    Lowell, D.; Parsley, W.; Bush,C; Zupo, D.

    2003-08-24

    Using previously published data on regulated and unregulated emissions, this paper will compare the environmental performance of current generation transit buses operated on compressed natural gas (CNG) to current generation transit buses operated on ultra low sulfur diesel fuel (ULSD) and incorporating diesel particulate filters (DPF). Unregulated emissions evaluated include toxic compounds associated with adverse health effects (carbonyl, PAH, NPAH, benzene) as well as PM particle count and size distribution. For all regulated and unregulated emissions, both technologies are shown to be comparable. DPF equipped diesel buses and CNG buses have virtually identical levels of PM mass emissions and particle number emissions. DPF-equipped diesel buses have lower HC and CO emissions and lower emissions of toxic substances such as benzene, carbonyls and PAHs than CNG buses. CNG buses have lower NOx emissions than DPF-equipped buses, though CNG bus NOx emissions are shown to be much more variable. In addition, this paper will compare the capital and operating costs of CNG and DPF-equipped buses. The cost comparison is primarily based on the experience of MTA New York City Transit in operating CNG buses since 1995 and DPF-equipped buses fueled with ULSD since 2001. Published data on the experience of other large transit agencies in operating CNG buses is used to validate the NYCT experience. The incremental cost (compared to ''baseline'' diesel) of operating a typical 200-bus depot is shown to be six times higher for CNG buses than for ''clean diesel'' buses. The contributors to this increased cost for CNG buses are almost equally split between increased capital costs for purchase of buses and installation of fueling infrastructure, and increased operating costs for purchase of fuel, bus maintenance, and fuel station maintenance.

  5. Idaho CERCLA Disposal Facility Complex Compliance Demonstration for DOE Order 435.1

    SciTech Connect (OSTI)

    J. Simonds

    2006-09-01

    This compliance demonstration document provides an analysis of the Idaho CERCLA Disposal Facility (ICDF) Complex compliance with DOE Order 435.1. The ICDF Complex includes the disposal facility (landfill), evaporation pond, admin facility, weigh scale, decon building, treatment systems, and various staging/storage areas. These facilities were designed and are being constructed to be compliant with DOE Order 435.1, Resource Conservation and Recovery Act Subtitle C, and Toxic Substances Control Act polychlorinated biphenyl design and construction standards. The ICDF Complex is designated as the central Idaho National Laboratory (INL) facilityyy for the receipt, staging/storage, treatment, and disposal of INL Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) waste streams. This compliance demonstration document discusses the conceptual site model for the ICDF Complex area. Within this conceptual site model, the selection of the area for the ICDF Complex is discussed. Also, the subsurface stratigraphy in the ICDF Complex area is discussed along with the existing contamination beneath the ICDF Complex area. The designs for the various ICDF Complex facilities are also included in this compliance demonstration document. These design discussions are a summary of the design as presented in the Remedial Design/Construction Work Plans for the ICDF landfill and evaporation pond and the Staging, Storage, Sizing, and Treatment Facility. Each of the major facilities or systems is described including the design criteria.

  6. ICDF Complex Remedial Action Work Plan

    SciTech Connect (OSTI)

    W. M. Heileson

    2006-12-01

    This Remedial Action Work Plan provides the framework for operation of the Idaho Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) Disposal Facility Complex (ICDF). This facility includes (a) an engineered landfill that meets the substantial requirements of DOE Order 435.1, Resource Conservation and Recovery Act Subtitle C, Idaho Hazardous Waste Management Act, and Toxic Substances Control Act polychlorinated biphenyl landfill requirements; (b) centralized receiving, inspections, administration, storage/staging, and treatment facilities necessary for CERCLA investigation-derived, remedial, and removal waste at the Idaho National Laboratory (INL) prior to final disposition in the disposal facility or shipment off-Site; and (c) an evaporation pond that has been designated as a corrective action management unit. The ICDF Complex, including a buffer zone, will cover approximately 40 acres, with a landfill disposal capacity of approximately 510,000 yd3. The ICDF Complex is designed and authorized to accept INL CERCLA-generated wastes, and includes the necessary subsystems and support facilities to provide a complete waste management system. This Remedial Action Work Plan presents the operational approach and requirements for the various components that are part of the ICDF Complex. Summaries of the remedial action work elements are presented herein, with supporting information and documents provided as appendixes to this work plan that contain specific detail about the operation of the ICDF Complex. This document presents the planned operational process based upon an evaluation of the remedial action requirements set forth in the Operable Unit 3-13 Final Record of Decision.

  7. Early-warning process/control for anaerobic digestion and biological nitrogen transformation processes: Batch, semi-continuous, and/or chemostat experiments. Final report

    SciTech Connect (OSTI)

    Hickey, R.

    1992-09-01

    The objective of this project was to develop and test an early-warning/process control model for anaerobic sludge digestion (AD). The approach was to use batch and semi-continuously fed systems and to assemble system parameter data on a real-time basis. Specific goals were to produce a real-time early warning control model and computer code, tested for internal and external validity; to determine the minimum rate of data collection for maximum lag time to predict failure with a prescribed accuracy and confidence in the prediction; and to determine and characterize any trends in the real-time data collected in response to particular perturbations to feedstock quality. Trends in the response of trace gases carbon monoxide and hydrogen in batch experiments, were found to depend on toxicant type. For example, these trace gases respond differently for organic substances vs. heavy metals. In both batch and semi-continuously feed experiments, increased organic loading lead to proportionate increases in gas production rates as well as increases in CO and H{sub 2} concentration. An analysis of variance of gas parameters confirmed that CO was the most sensitive indicator variable by virtue of its relatively larger variance compared to the others. The other parameters evaluated including gas production, methane production, hydrogen, carbon monoxide, carbon dioxide and methane concentration. In addition, a relationship was hypothesized between gaseous CO concentration and acetate concentrations in the digester. The data from semicontinuous feed experiments were supportive.

  8. Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. [Salmonella typhimurium; Escherichia coli; Sarcina lutea; Staphylococcus aureus; Streptococcus lactis; Streptococcus faecalis

    SciTech Connect (OSTI)

    Dahl, T.A.; Midden, W.R. ); Hartman, P.E. )

    1989-04-01

    Gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. Killing curves for Salmonella typhimurium and Escherichia coli strains were indicative of multihit killing, whereas curves for Sarcina lutea, Staphylococcus aureus, Streptococcus lactis, and Streptococcus faecalis exhibited single-hit kinetics. The S. typhimurium deep rough strain TA1975, which lacks nearly all of the cell wall lipopolysaccharide coat and manifests concomitant enhancement of penetration by some exogenous substances, responded to singlet oxygen with initially faster inactivation than did the S. typhimurium wild-type strain, although the maximum rates of killing appeared to be quite similar. The structure of the cell wall thus plays an important role in susceptibility to singlet oxygen. The outer membrane-lipopolysaccharide portion of the gram-negative cell wall initially protects the bacteria from extracellular singlet oxygen, although it may also serve as a source for secondary reaction products which accentuate the rates of cell killing. S. typhimurium and E. coli strains lacking the cellular antioxidant, glutathione, showed no difference from strains containing glutathione in response to the toxic effects of singlet oxygen. Strains of Sarcina lutea and Staphylococcus aureus that contained carotenoids, however, were far more resistant to singlet oxygen lethality than were both carotenoidless mutants of the same species and other gram-positive species lacking high levels of protective carotenoids.

  9. Use of information resources by the state of Tennessee in risk assessment applications

    SciTech Connect (OSTI)

    Bashor, B.S.

    1990-12-31

    The major resources used by the Bureau of Environment, and Environmental Epidemiology (EEP) for risk assessment are: the Integrated Risk Information System (IRIS), Health and Environmental Effects Summary Table (HEAST), Agency for Toxic Substances and disease Registry (ATSDR) Toxicological Profiles, databases at the National Library of Medicine (NLM), World Health Organization (WHO) ENvironmental Criteria, and documents that the Environmental Protection Agency (EPA) has published on Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) risk assessment activities. The Risk Assessment Review has been helpful in providing information about availability of new documents or information. No systematic method has been made available to us to locate information resources. IRIS User`s Support has been helpful in making appropriate and timely referrals. Most other EPA resources were located by serendipity and persistence. The CERCLA methodology for risk assessments is being used in environmental programs, and at present, one person is responsible for all risk assessment activities in the department, but plans are underway to train one or two people from each program area. 2 figs.

  10. Role of soil microbial processes in integrated pest management

    SciTech Connect (OSTI)

    Francis, A.J.

    1987-01-01

    Soil microorganisms play a significant role in the carbon, nitrogen, phosphorus, and sulfur cycles in nature and are critical to the functioning of ecosystems. Microorganisms affect plant growth directly by regulating the availability of plant nutrients in soil, or indirectly by affecting the population dynamics of plant pathogens in soil. Any adverse effect on soil microorganisms or on the microbial processes will affect the soil fertility, availability of plant nutrients and the overall biogeochemical cycling of elements in nature. Soil microorganisms are responsible for the degradation and detoxification of pesticides; they control many insect pests, nematodes, and other plant pathogenic microorganisms by parasitism, competition, production of antibiotics and other toxic substances. Also, they regulate the availability of major and minor nutrients as well as essential elements. The long-term effects of continuous and, in some instances, excessive application of pesticides on soil fertility is not fully understood. Although much information is available on the integrated pest management (IPM) system, we have very little understanding of the extent of soil microbial processes which modulate the overall effectiveness of various strategies employed in IPM. The purpose of this paper is to review briefly the key microbial processes and their relationship to the IPM system.

  11. Sulforaphane prevents pulmonary damage in response to inhaled arsenic by activating the Nrf2-defense response

    SciTech Connect (OSTI)

    Zheng, Yi; Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona, 1703 East Mabel Street, Tucson, AZ 85721 ; Tao, Shasha; Lian, Fangru; Chau, Binh T.; Chen, Jie; Sun, Guifan; Fang, Deyu; Lantz, R. Clark; Arizona Cancer Center, University of Arizona, 1515 North Campbell Avenue, Tucson, AZ 85724 ; Zhang, Donna D.

    2012-12-15

    Exposure to arsenic is associated with an increased risk of lung disease. Novel strategies are needed to reduce the adverse health effects associated with arsenic exposure in the lung. Nrf2, a transcription factor that mediates an adaptive cellular defense response, is effective in detoxifying environmental insults and prevents a broad spectrum of diseases induced by environmental exposure to harmful substances. In this report, we tested whether Nrf2 activation protects mice from arsenic-induced toxicity. We used an in vivo arsenic inhalation model that is highly relevant to low environmental human exposure to arsenic-containing dusts. Two-week exposure to arsenic-containing dust resulted in pathological alterations, oxidative DNA damage, and mild apoptotic cell death in the lung; all of which were blocked by sulforaphane (SF) in an Nrf2-dependent manner. Mechanistically, SF-mediated activation of Nrf2 alleviated inflammatory responses by modulating cytokine production. This study provides strong evidence that dietary intervention targeting Nrf2 activation is a feasible approach to reduce adverse health effects associated with arsenic exposure. -- Highlights: ? Exposed to arsenic particles and/or SF have elevated Nrf2 and its target genes. ? Sulforaphane prevents pathological alterations, oxidative damage and cell death. ? Sulforaphane alleviates infiltration of inflammatory cells into the lungs. ? Sulforaphane suppresses arsenic-induced proinflammatory cytokine production.

  12. Risk analyses for disposing of nonhazardous oil field wastes in salt caverns

    SciTech Connect (OSTI)

    Tomasko, D.; Elcock, D.; Veil, J.

    1997-09-01

    Argonne National Laboratory (ANL) has completed an evaluation of the possibility that adverse human health effects (carcinogenic and noncarcinogenic) could result from exposure to contaminants released from nonhazardous oil field wastes (NOW) disposed of in domal salt caverns. In this assessment, several steps were used to evaluate potential human health risks: identifying potential contaminants of concern; determining how humans could be exposed to these contaminants; assessing the contaminants` toxicities; estimating contaminant intakes; and, finally, calculating human cancer and noncancer risks. Potential human health risks associated with hazardous substances (arsenic, benzene, cadmium, and chromium) in NOW were assessed under four postclosure cavern release scenarios: inadvertent cavern intrusion, failure of the cavern seal, failure of the cavern through cracks or leaky interbeds, and a partial collapse of the cavern roof. To estimate potential human health risks for these scenarios, contaminant concentrations at the receptor were calculated using a one-dimensional solution to an advection/dispersion equation that included first order degradation. Even under worst-case conditions, the risks have been found to be within the US EPA target range for acceptable exposure levels. From a human health risk perspective, salt caverns can provide an acceptable disposal method for NOW.

  13. An analysis of the composition and metal contamination of plastics from waste electrical and electronic equipment (WEEE)

    SciTech Connect (OSTI)

    Stenvall, Erik; Tostar, Sandra; Boldizar, Antal; Foreman, Mark R.StJ.; Mller, Kenneth

    2013-04-15

    The compositions of three WEEE plastic batches of different origin were investigated using infrared spectroscopy, and the metal content was determined with inductively coupled plasma. The composition analysis of the plastics was based mainly on 14 samples collected from a real waste stream, and showed that the major constituents were high impact polystyrene (42 wt%), acrylonitrilebutadienestyrene copolymer (38 wt%) and polypropylene (10 wt%). Their respective standard deviations were 21.4%, 16.5% and 60.7%, indicating a considerable variation even within a single batch. The level of metal particle contamination was found to be low in all samples, whereas wood contamination and rubber contamination were found to be about 1 wt% each in most samples. In the metal content analysis, iron was detected at levels up to 700 ppm in the recyclable waste plastics fraction, which is of concern due to its potential to catalyse redox reactions during melt processing and thus accelerate the degradation of plastics during recycling. Toxic metals were found only at very low concentrations, with the exception of lead and cadmium which could be detected at 200 ppm and 70 ppm levels, respectively, but these values are below the current threshold limits of 1000 ppm and 100 ppm set by the Restriction of Hazardous Substances directive.

  14. Federal program for regulating highly hazardous materials finally takes off

    SciTech Connect (OSTI)

    Lessard, P.C. [Block Environmental Services Inc., Pleasant Hill, CA (United States)

    1996-11-01

    The Risk Management Program (RMP) rule, Section 112(r) of the Clean Air Act (CAA), was signed on May 24 and finalized on June 20. RMP is one of the most comprehensive, technically based regulatory programs for preventing, detecting and responding to accidental hazardous materials releases to have been issued in recent times. Although facilities have three years to comply, EPA estimates that the rule will affect an estimated 66,000 facilities that store highly hazardous or acutely toxic materials. The 1990 CAA Amendments are designed to prevent accidental releases of highly hazardous chemicals from stationary sources. Two significant regulatory programs that have emerged from the revised CAA are the Process Safety Management (PSM) standard and RMP. PSM is designed to protect employees and regulated by the Occupational Safety and Health Administration. RMP`s purpose is to protect the public and the environment from highly hazardous chemicals. It authorizes EPA to create a list of substances (distinct from the list generated under PSM) known to cause serious adverse effects and to implement a program for accidental chemical release prevention.

  15. Experimental studies of mitochondrial function in CADASIL vascular smooth muscle cells

    SciTech Connect (OSTI)

    Viitanen, Matti; Department of Geriatrics, Turku City Hospital and University of Turku, Turku ; Sundstrm, Erik; Baumann, Marc; Tikka, Saara; Karolinska Institutet Alzheimer's Disease Research Center, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm

    2013-02-01

    Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is a familiar fatal progressive degenerative disorder characterized by cognitive decline, and recurrent stroke in young adults. Pathological features include a dramatic reduction of brain vascular smooth muscle cells and severe arteriopathy with the presence of granular osmophilic material in the arterial walls. Here we have investigated the cellular and mitochondrial function in vascular smooth muscle cell lines (VSMCs) established from CADASIL mutation carriers (R133C) and healthy controls. We found significantly lower proliferation rates in CADASIL VSMC as compared to VSMC from controls. Cultured CADASIL VSMCs were not more vulnerable than control cells to a number of toxic substances. Morphological studies showed reduced mitochondrial connectivity and increased number of mitochondria in CADASIL VSMCs. Transmission electron microscopy analysis demonstrated increased irregular and abnormal mitochondria in CADASIL VSMCs. Measurements of mitochondrial membrane potential (??{sub m}) showed a lower percentage of fully functional mitochondria in CADASIL VSMCs. For a number of genes previously reported to be changed in CADASIL VSMCs, immunoblotting analysis demonstrated a significantly reduced SOD1 expression. These findings suggest that alteration of proliferation and mitochondrial function in CADASIL VSMCs might have an effect on vital cellular functions important for CADASIL pathology. -- Highlights: ? CADASIL is an inherited disease of cerebral vascular cells. ? Mitochondrial dysfunction has been implicated in the pathogenesis of CADASIL. ? Lower proliferation rates in CADASIL VSMC. ? Increased irregular and abnormal mitochondria and lower mitochondrial membrane potential in CADASIL VSMCs. ? Reduced mitochondrial connectivity and increased number of mitochondria in CADASIL VSMCs.

  16. PINS Spectrum Identification Guide

    SciTech Connect (OSTI)

    A.J. Caffrey

    2012-03-01

    The Portable Isotopic Neutron SpectroscopyPINS, for shortsystem identifies the chemicals inside munitions and containers without opening them, a decided safety advantage if the fill chemical is a hazardous substance like a chemical warfare agent or an explosive. The PINS Spectrum Identification Guide is intended as a reference for technical professionals responsible for the interpretation of PINS gamma-ray spectra. The guide is divided into two parts. The three chapters that constitute Part I cover the science and technology of PINS. Neutron activation analysis is the focus of Chapter 1. Chapter 2 explores PINS hardware, software, and related operational issues. Gamma-ray spectral analysis basics are introduced in Chapter 3. The six chapters of Part II cover the identification of PINS spectra in detail. Like the PINS decision tree logic, these chapters are organized by chemical element: phosphorus-based chemicals, chlorine-based chemicals, etc. These descriptions of hazardous, toxic, and/or explosive chemicals conclude with a chapter on the identification of the inert chemicals, e.g. sand, used to fill practice munitions.

  17. Synthesis of Metal Oxide Nanomaterials for Chemical Sensors by Molecular Beam Epitaxy

    SciTech Connect (OSTI)

    Nandasiri, Manjula I.; Kuchibhatla, Satyanarayana V N T; Thevuthasan, Suntharampillai

    2013-12-01

    Since the industrial revolution, detection and monitoring of toxic matter, chemical wastes, and air pollutants has become an important environmental issue. Thus, it leads to the development of chemical sensors for various environmental applications. The recent disastrous oil spills over the near-surface of ocean due to the offshore drilling emphasize the use of chemical sensors for prevention and monitoring of the processes that might lead to these mishaps.1, 2 Chemical sensors operated on a simple principle that the sensing platform undergoes a detectable change when exposed to the target substance to be sensed. Among all the types of chemical sensors, solid state gas sensors have attracted a great deal of attention due to their advantages such as high sensitivity, greater selectivity, portability, high stability and low cost.3, 4 Especially, semiconducting metal oxides such as SnO2, TiO2, and WO3 have been widely used as the active sensing platforms in solid state gas sensors.5 For the enhanced properties of solid state gas sensors, finding new sensing materials or development of existing materials will be needed. Thus, nanostructured materials such as nanotubes,6-8 nanowires,9-11 nanorods,12-15 nanobelts,16, 17 and nano-scale thin films18-23 have been synthesized and studied for chemical sensing applications.

  18. Health assessment for Cedartown Industries, Cedartown, Polk County, Georgia, Region 4. CERCLIS No. GAD95840674. Preliminary report

    SciTech Connect (OSTI)

    Not Available

    1990-01-29

    The Cedartown Industries, Inc. site has been proposed for the National Priorities List (NPL) by the U.S. Environmental Protection Agency (EPA). The 7-acre site is located in Cedartown, Polk County, Georgia, in the northwest part of the State. In 1986, one sediment sample from an on-site impoundment and two soil samples (one background) were collected on-site and analyzed by EPD. Only Extraction Procedure (EP) Toxicity testing was performed on these samples. The test which measures the concentration of leachate generated from the soil showed leachate with lead concentrations up to 720 parts per million (ppm) and cadmium concentrations up to 1.4 ppm. Based on the information reviewed, ATSDR has concluded that the site is of potential public health concern because humans may be exposed to hazardous substances at concentrations that may result in adverse health effects. As noted in the Human Exposure Pathways Section above, human exposure to lead may occur via ingestion of, inhalation of, and dermal contact with surface water, sediments, soils, ground water, air, and food-chain entities.

  19. Health effects associated with energy conservation measures in commercial buildings

    SciTech Connect (OSTI)

    Stenner, R.D.; Baechler, M.C.

    1990-09-01

    Indoor air quality can be impacted by hundreds of different chemicals. More than 900 different organic compounds alone have been identified in indoor air. Health effects that could arise from exposure to individual pollutants or mixtures of pollutants cover the full range of acute and chronic effects, including largely reversible responses, such as rashes and irritations, to the irreversible toxic and carcinogenic effects. These indoor contaminants are emitted from a large variety of materials and substances that are widespread components of everyday life. Pacific Northwest Laboratory conducted a search of the peer-reviewed literature on health effects associated with indoor air contaminants for the Bonneville Power Administration to aid the agency in the preparation of environmental documents. Results are reported in two volumes. Volume 1 summarizes the results of the search of the peer-reviewed literature on health effects associated with a selected list of indoor air contaminants. In addition, the report discusses potential health effects of polychlorinated biphenyls and chlorofluorocarbons. All references to the literature reviewed are found in this document Volume 2. Volume 2 provides detailed information from the literature reviewed, summarizes potential health effects, reports health hazard ratings, and discusses quantitative estimates of carcinogenic risk in humans and animals. Contaminants discussed in this report are those that; have been measured in the indoor air of a public building; have been measured (significant concentrations) in test situations simulating indoor air quality (as presented in the referenced literature); and have a significant hazard rating. 38 refs., 7 figs., 23 tabs.

  20. Health effects associated with energy conservation measures in commercial buildings

    SciTech Connect (OSTI)

    Stenner, R.D.; Baechler, M.C.

    1990-09-01

    Indoor air quality can conceivably be impacted by hundreds of different chemicals. More than 900 different organic compounds alone have been identified in indoor air. The health effects that could potentially arise from exposure to individual pollutants or mixtures of pollutants cover the full range of acute and chronic effects, including largely reversible responses, such as rashes and irritations, as well as irreversible toxic and carcinogenic effects. These indoor contaminants are emitted from a large variety of materials and substances that are widespread components of everyday life. Pacific Northwest Laboratory conducted a search of the peer-reviewed literature on health effects associated with indoor air contaminants for the Bonneville Power Administration to aid the agency in the preparation of environmental documents. The results of this search are reported in two volumes. Volume 1 is a summary of the results of the literature search; Volume 2 is the complete results of the literature search and contains all references to the material reviewed. 16 tabs.

  1. Selective Filtration of Gadolinium Trichloride for Use in Neutron Detection in Large Water Cherenkov Detectors

    SciTech Connect (OSTI)

    Vagins, Mark R.

    2013-04-10

    Water Cherenkov detectors have been used for many years as inexpensive, effective detectors for neutrino interactions and nucleon decay searches. While many important measurements have been made with these detectors a major drawback has been their inability to detect the absorption of thermal neutrons. We believe an inexpensive, effective technique could be developed to overcome this situation via the addition to water of a solute with a large neutron cross section and energetic gamma daughters which would make neutrons detectable. Gadolinium seems an excellent candidate especially since in recent years it has become very inexpensive, now less than $8 per kilogram in the form of commercially-available gadolinium trichloride, GdCl{sub 3}. This non-toxic, non-reactive substance is highly soluble in water. Neutron capture on gadolinium yields a gamma cascade which would be easily seen in detectors like Super-Kamiokande. We have been investigating the use of GdCl{sub 3} as a possible upgrade for the Super-Kamiokande detector with a view toward improving its performance as a detector for atmospheric neutrinos, supernova neutrinos, wrong-sign solar neutrinos, reactor neutrinos, proton decay, and also as a target for the coming T2K long-baseline neutrino experiment. This focused study of selective water filtration and GdCl{sub 3} extraction techniques, conducted at UC Irvine, followed up on highly promising benchtop-scale and kiloton-scale work previously carried out with the assistance of 2003 and 2005 Advanced Detector Research Program grants.

  2. Wireless Sensor Networks - Node Localization for Various Industry Problems

    SciTech Connect (OSTI)

    Derr, Kurt; Manic, Milos

    2015-06-01

    Fast, effective monitoring following airborne releases of toxic substances is critical to mitigate risks to threatened population areas. Wireless sensor nodes at fixed predetermined locations may monitor such airborne releases and provide early warnings to the public. A challenging algorithmic problem is determining the locations to place these sensor nodes while meeting several criteria: 1) provide complete coverage of the domain, and 2) create a topology with problem dependent node densities, while 3) minimizing the number of sensor nodes. This manuscript presents a novel approach to determining optimal sensor placement, Advancing Front mEsh generation with Constrained dElaunay Triangulation and Smoothing (AFECETS) that addresses these criteria. A unique aspect of AFECETS is the ability to determine wireless sensor node locations for areas of high interest (hospitals, schools, high population density areas) that require higher density of nodes for monitoring environmental conditions, a feature that is difficult to find in other research work. The AFECETS algorithm was tested on several arbitrary shaped domains. AFECETS simulation results show that the algorithm 1) provides significant reduction in the number of nodes, in some cases over 40%, compared to an advancing front mesh generation algorithm, 2) maintains and improves optimal spacing between nodes, and 3) produces simulation run times suitable for real-time applications.

  3. (Low frequency electromagnetic fields and public health)

    SciTech Connect (OSTI)

    Aldrich, T.E.

    1988-05-23

    The traveler participated in the IARC-sponsored workshop entitled Extremely Low Frequency Electromagnetic Fields (EMF) and Public Health'' where he delivered the keynote address. This address set the stage for deliberations among the EMF public health professionals regarding strategies for international collaborative work on this topic. Strong emphasis was placed in explicit exposure monitoring. The traveler also participated in the Tenth Yves Biraud Seminar on rare-event surveillance as a sentinel system for detection potential environmental hazards. He presented an invited paper describing a means for making rapid, preliminary decisions regarding potential health impacts due to contamination of the environment around point sources of toxic substances. He served as the symposium's expert on numerical techniques on the use of spatial and temporal aggregation of rare health events. There is considerable variation among countries in emphasis on application of sentinel systems and application of sentinel systems and data gathering. France has a highly automated, statistically-sophisticated system involving individual physician reporting of specific reportable infectious diseases to a central location. The European Common Market nations are sold on this concept and are supporting the development of an internationally coordinated system.

  4. Surveillance study of health effects associated with cleanup of a hazardous waste site, Ralph Gray Trucking Company (a/k/a Westminster Tract Number 2633), Westminster, Orange County, California, Region 9: CERCLIS number CAD981995947

    SciTech Connect (OSTI)

    Hoshiko, S.; Underwood, M.C.; Smith, D.; DeLorenze, G.; Neuhaus, J.

    1999-04-01

    Excavation of a Superfund site, the Ralph Gray Truncking Company located in Westminster Orange County, California was anticipated to release sulfur dioxide and other chemicals. The California Department of Health Services, under cooperative agreement with the Agency for Toxic Substances and Disease Registry, conducted a surveillance study to assess whether illnesses were associated with cleanup activities. A panel primarily composed of more sensitive persons (n = 36) was selected to report daily respiratory symptoms and odors. Exposures included sulfur dioxide (SO{sub 2}) measurements and daily tonnage of waste removed. Analysis used Conditional Likelihood Regression and Generalized Estimating Equations (GEE) methods. Levels of SO{sub 2} were generally higher than usual ambient air, at times exceeding levels which can cause health effects among asthmatics in laboratory settings. Wheeze and cough were significantly associated with tonnage of waste removed, especially on days when the highest amounts of waste were removed. Upper respiratory symptoms were found to be associated with SO{sub 2}, and weak relationships were found with nausea and burning nose and SO{sub 2}.

  5. Results of the radiological and chemical characterization of surface impoundments 3539 and 3540 at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    Murray, M.E.; Rose, D.A.; Brown, K.S.; Winton, W.; Dean, R.A.; Coe, R.H. III

    1998-03-01

    A radiological and chemical characterization survey of impoundments 3539 and 3540 at the Oak Ridge National Laboratory (ORNL) was conducted during December 1997. Impoundments 3539 and 3540 are located in the Surface Impoundments Operable Unit (SIOU) of Waste Area Group 1. The investigation was performed by the Measurement Applications and Development Group of the Life Sciences Division of ORNL at the request of the Department of Energy (DOE) Office of Environmental Restoration. Sampling was conducted in order to quantify the presence of polychlorinated biphenyls (PCBs), Resource Conservation and Recovery Act (RCRA) constituents, and other contaminants of interest in support of the Comprehensive Environmental Response, Compensation and Liability Act (CERCLA) remediation for the SIOU> The survey included collection of sediment/clay samples, quality control blank water samples and equipment rinsate samples for chemical and radiological analysis. Results show the samples contain traces of various organic, inorganic, and radioactive materials. Of particular interest are PCB values which demonstrate the impoundments are not regulated under the Toxic Substances Control Act.

  6. Wireless Sensor Networks - Node Localization for Various Industry Problems

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Derr, Kurt; Manic, Milos

    2015-06-01

    Fast, effective monitoring following airborne releases of toxic substances is critical to mitigate risks to threatened population areas. Wireless sensor nodes at fixed predetermined locations may monitor such airborne releases and provide early warnings to the public. A challenging algorithmic problem is determining the locations to place these sensor nodes while meeting several criteria: 1) provide complete coverage of the domain, and 2) create a topology with problem dependent node densities, while 3) minimizing the number of sensor nodes. This manuscript presents a novel approach to determining optimal sensor placement, Advancing Front mEsh generation with Constrained dElaunay Triangulation and Smoothingmore » (AFECETS) that addresses these criteria. A unique aspect of AFECETS is the ability to determine wireless sensor node locations for areas of high interest (hospitals, schools, high population density areas) that require higher density of nodes for monitoring environmental conditions, a feature that is difficult to find in other research work. The AFECETS algorithm was tested on several arbitrary shaped domains. AFECETS simulation results show that the algorithm 1) provides significant reduction in the number of nodes, in some cases over 40%, compared to an advancing front mesh generation algorithm, 2) maintains and improves optimal spacing between nodes, and 3) produces simulation run times suitable for real-time applications.« less

  7. Evaluation of bulk paint worker exposure to solvents at household hazardous waste collection events

    SciTech Connect (OSTI)

    Cameron, M.

    1995-09-01

    In fiscal year 93/94, over 250 governmental agencies were involved in the collection of household hazardous wastes in the State of California. During that time, over 3,237,000 lbs. of oil based paint were collected in 9,640 drums. Most of this was in lab pack drums, which can only hold up to 20 one gallon cans. Cost for disposal of such drums is approximately $1000. In contrast, during the same year, 1,228,000 lbs. of flammable liquid were collected in 2,098 drums in bulk form. Incineration of bulked flammable liquids is approximately $135 per drum. Clearly, it is most cost effective to bulk flammable liquids at household hazardous waste events. Currently, this is the procedure used at most Temporary Household Hazardous Waste Collection Facilities (THHWCFs). THHWCFs are regulated by the Department of Toxic Substances Control (DTSC) under the new Permit-by Rule Regulations. These regulations specify certain requirements regarding traffic flow, emergency response notifications and prevention of exposure to the public. The regulations require that THHWCF operators bulk wastes only when the public is not present. [22 CCR, section 67450.4 (e) (2) (A)].Santa Clara County Environmental Health Department sponsors local THHWCF`s and does it`s own bulking. In order to save time and money, a variance from the regulation was requested and an employee monitoring program was initiated to determine actual exposure to workers. Results are presented.

  8. Human and animal health risk assessments of chemicals in the food chain: Comparative aspects and future perspectives

    SciTech Connect (OSTI)

    Dorne, J.L.C.M.; Fink-Gremmels, J.

    2013-08-01

    Chemicals from anthropogenic and natural origins enter animal feed, human food and water either as undesirable contaminants or as part of the components of a diet. Over the last five decades, considerable efforts and progress to develop methodologies to protect humans and animals against potential risks associated with exposure to such potentially toxic chemicals have been made. This special issue presents relevant methodological developments and examples of risk assessments of undesirable substances in the food chain integrating the animal health and the human health perspective and refers to recent Opinions of the Scientific Panel on Contaminants in the Food Chain (CONTAM) of the European Food Safety Authority (EFSA). This introductory review aims to give a comparative account of the risk assessment steps used in human health and animal health risk assessments for chemicals in the food chain and provides a critical view of the data gaps and future perspectives for this cross-disciplinary field. - Highlights: ? Principles of human and animal health risk assessment. ? Data gaps for each step of animal health risk assessment. ? Implications of animal risk assessment on human risk assessment. ? Future perspectives on chemical risk assessment.

  9. An integrated approach for prospectively investigating a mode-of-action for rodent liver effects

    SciTech Connect (OSTI)

    LeBaron, Matthew J.; Geter, David R.; Rasoulpour, Reza J.; Gollapudi, B. Bhaskar; Thomas, Johnson; Murray, Jennifer; Kan, H. Lynn; Wood, Amanda J.; Elcombe, Cliff; Vardy, Audrey; McEwan, Jillian; Terry, Claire; Billington, Richard

    2013-07-15

    Registration of new plant protection products (e.g., herbicide, insecticide, or fungicide) requires comprehensive mammalian toxicity evaluation including carcinogenicity studies in two species. The outcome of the carcinogenicity testing has a significant bearing on the overall human health risk assessment of the substance and, consequently, approved uses for different crops across geographies. In order to understand the relevance of a specific tumor finding to human health, a systematic, transparent, and hypothesis-driven mode of action (MoA) investigation is, appropriately, an expectation by the regulatory agencies. Here, we describe a novel approach of prospectively generating the MoA data by implementing additional end points to the standard guideline toxicity studies with sulfoxaflor, a molecule in development. This proactive MoA approach results in a more robust integration of molecular with apical end points while minimizing animal use. Sulfoxaflor, a molecule targeting sap-feeding insects, induced liver effects (increased liver weight due to hepatocellular hypertrophy) in an initial palatability probe study for selecting doses for subsequent repeat-dose dietary studies. This finding triggered the inclusion of dose-response investigations of the potential key events for rodent liver carcinogenesis, concurrent with the hazard assessment studies. As predicted, sulfoxaflor induced liver tumors in rats and mice in the bioassays. The MoA data available by the time of the carcinogenicity finding supported the conclusion that the carcinogenic potential of sulfoxaflor was due to CAR/PXR nuclear receptor activation with subsequent hepatocellular proliferation. This MoA was not considered to be relevant to humans as sulfoxaflor is unlikely to induce hepatocellular proliferation in humans and therefore would not be a human liver carcinogen. - Highlights: We prospectively generated MoA data into standard guideline toxicity studies. A proactive MoA approach integrates all end points while minimizing animal use. MoA data predicted the rodent carcinogenicity of sulfoxaflor via CAR/PXR. Liver MoA was considered not relevant to humans and hence not a human carcinogen.

  10. Effects of two Asian sand dusts transported from the dust source regions of Inner Mongolia and northeast China on murine lung eosinophilia

    SciTech Connect (OSTI)

    He, Miao; Ichinose, Takamichi; Song, Yuan; Yoshida, Yasuhiro; Arashidani, Keiichi; Yoshida, Seiichi; Liu, Boying; Nishikawa, Masataka; Takano, Hirohisa; and others

    2013-11-01

    The quality and quantity of toxic materials adsorbed onto Asian sand dust (ASD) are different based on dust source regions and passage routes. The aggravating effects of two ASDs (ASD1 and ASD2) transported from the source regions of Inner Mongolia and northeast China on lung eosinophilia were compared to clarify the role of toxic materials in ASD. The ASDs contained different amounts of lipopolysaccharides (LPS) and ?-glucan (ASD1 < ASD2) and SiO{sub 2} (ASD1 > ASD2). CD-1 mice were instilled intratracheally with ASD1, ASD2 and/or ovalbumin (OVA) four times at 2-week intervals. ASD1 and ASD2 enhanced eosinophil recruitment induced by OVA in the submucosa of the airway, with goblet cell proliferation in the bronchial epithelium. ASD1 and ASD2 synergistically increased OVA-induced eosinophil-relevant cytokines interleukin-5 (IL-5), IL-13 (ASD1 < ASD2) and chemokine eotaxin (ASD1 > ASD2) in bronchoalveolar lavage fluid. ASD2 aggravating effects on lung eosinophilia were greater than ASD1. The role of LPS and ?-glucan in ASD2 on the production of pro-inflammatory mediators was assessed using in vitro bone marrow-derived macrophages (BMDMs) from wild type, Toll-like receptor 2-deficient (TLR2 ?/?), TLR4 ?/?, and MyD88 ?/? mice (on Balb/c background). ASD2-stimulated TLR2 ?/? BMDMs enhanced IL-6, IL-12, TNF-?, MCP-1 and MIP-1? secretion compared with ASD2-stimulated TLR4 ?/? BMDMs. Protein expression from ASD2-stimulated MyD88 ?/? BMDM were very low or undetectable. The in vitro results indicate that lung eosinophilia caused by ASD is TLR4 dependent. Therefore, the aggravation of OVA-related lung eosinophilia by ASD may be dependent on toxic substances derived from microbes, such as LPS, rather than SiO{sub 2}. - Highlights: Asian sand dust (ASD) from the deserts of China causes serious respiratory problems. The aggravating effects of two ASDs on lung eosinophilia were compared. The ASDs contained different LPS and ?-glucan (ASD1 < ASD2) and SiO{sub 2} (ASD1 > ASD2). The ASD2 aggravating effects on lung eosinophilia were greater than ASD1. The aggravation of lung eosinophilia may be dependent on LPS in ASD, rather than SiO{sub 2}.

  11. System and method for 100% moisture and basis weight measurement of moving paper

    DOE Patents [OSTI]

    Hernandez, Jose E. (Livermore, CA); Koo, Jackson C. (San Ramon, CA)

    2002-01-01

    A system for characterizing a set of properties for a moving substance are disclosed. The system includes: a first near-infrared linear array; a second near-infrared linear array; a first filter transparent to a first absorption wavelength emitted by the moving substance and juxtaposed between the substance and the first array; a second filter blocking the first absorption wavelength emitted by the moving substance and juxtaposed between the substance and the second array; and a computational device for characterizing data from the arrays into information on a property of the substance. The method includes the steps of: filtering out a first absorption wavelength emitted by a substance; monitoring the first absorption wavelength with a first near-infrared linear array; blocking the first wavelength from reaching a second near-infrared linear array; and characterizing data from the arrays into information on a property of the substance.

  12. Paint for detection of corrosion and warning of chemical and radiological attack

    DOE Patents [OSTI]

    Farmer, Joseph C. (Tracy, CA)

    2010-08-24

    A system for warning of corrosion, chemical, or radiological substances. The system comprises painting a surface with a paint or coating that includes an indicator material and monitoring the surface for indications of the corrosion, chemical, or radiological substances.

  13. 1

    Office of Environmental Management (EM)

    and, as applicable, contaminated media such that they do not exceed a total excess cancer risk of 10 -5 for carcinogenic substances and, for non-carcinogenic substances, a...

  14. Draft Closure Plan

    Office of Environmental Management (EM)

    and, as applicable, contaminated media such that they do not exceed a total excess cancer risk of 10 -5 for carcinogenic substances and, for non-carcinogenic substances, a...

  15. Surface with two paint strips for detection and warning of chemical warfare and radiological agents

    DOE Patents [OSTI]

    Farmer, Joseph C.

    2013-04-02

    A system for warning of corrosion, chemical, or radiological substances. The system comprises painting a surface with a paint or coating that includes an indicator material and monitoring the surface for indications of the corrosion, chemical, or radiological substances.

  16. Superhydrophobic Transparent Glass Thin Films - Energy Innovation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    surfaces require frequent maintenance andor replacement. And some of the processes use toxic chemicals or produce toxic wastes, leading to disposal problems. DescriptionThe basic...

  17. Microsoft Word - DOE-ID-INL-15-052.docx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    efficient, water efficient, are bio- based in content, environmentally preferable, non-ozone depleting, have recycled content, or are non-toxic or less-toxic alternatives. New...

  18. NREL: Biomass Research - Biochemical Conversion Capabilities

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    environment. This process of pH adjustment, known as conditioning, aims to minimize sugar losses and promote low hydrolyzate toxicity by removing toxic byproducts that...

  19. Pro-Inflammatory Cytokine Responses to Exposure to Diesel Soot...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Relationship Between Composition and Toxicity of Engine Emissions Comparative Toxicity of Combined Particle and Semi-Volatile Organic Fractioins of Gasoline and Diesel Emissions

  20. Comparison of direct exposure of human lung cells to modern engine...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Relationship Between Composition and Toxicity of Engine Emissions Comparative Toxicity of Combined Particle and Semi-Volatile Organic Fractioins of Gasoline and Diesel Emissions

  1. Phase 2 Study of Docetaxel, Cisplatin, and Concurrent Radiation...

    Office of Scientific and Technical Information (OSTI)

    whereas myelosuppression and renal injury were minimal. No patients died of toxicity. ... mucositis, was common, late toxicity, such as laryngoesophageal dysfunction, was minimal. ...

  2. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... whereas myelosuppression and renal injury were minimal. No patients died of toxicity. ... mucositis, was common, late toxicity, such as laryngoesophageal dysfunction, was minimal. ...

  3. Biosafety Links | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Infectious Agents Microwave Safety MSDSs for Infectious Substances Organizations Prions Recombinant DNA Regulated Medical Waste Safety Manuals Select Agents Shipping...

  4. Exhibits - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    supplements, healthy hearts, safety awards, massage therapy, hypnosis, acupuncture, diabetes, welding safety, stranger dangers, substance abuse, protective clothing alternatives,...

  5. Exhibitor Information - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    supplements, healthy hearts, safety awards, massage therapy, hypnosis, acupuncture, diabetes, welding safety, stranger dangers, substance abuse, protective clothing alternatives,...

  6. Toxin-eating bacteria and bioremediation

    DOE Patents [OSTI]

    Church, George M; Dantas, Gautam; Sommer, Morten O

    2014-02-25

    Methods are provided for reducing a level of one or more antibiotics from an antibiotic-contaminated substance. An organism that can utilize the one or more antibiotics as a carbon source is cultured in the presence of the antibiotic-contaminated substance for a sufficient amount of time to reduce the level of one or more antibiotics from the antibiotic-contaminated substance.

  7. Annual Report on Waste Generation and Waste Minimization Progress, 1991--1992

    SciTech Connect (OSTI)

    Not Available

    1994-02-01

    This report is DOE`s first annual report on waste generation and waste minimization progress. Data presented in this report were collected from all DOE sites which met minimum threshold criteria established for this report. The fifty-seven site submittals contained herein represent data from over 100 reporting sites within 25 states. Radioactive, hazardous and sanitary waste quantities and the efforts to minimize these wastes are highlighted within the fifty-seven site submittals. In general, sites have made progress in moving beyond the planning phase of their waste minimization programs. This is evident by the overall 28 percent increase in the total amount of materials recycled from 1991 to 1992, as well as individual site initiatives. During 1991 and 1992, DOE generated a total of 279,000 cubic meters of radioactive waste and 243,000 metric tons of non-radioactive waste. These waste amounts include significant portions of process wastewater required to be reported to regulatory agencies in the state of Texas and the state of Tennessee. Specifically, the Pantex Plant in Texas treats an industrial wastewater that is considered by the Texas Water Commission to be a hazardous waste. In 1992, State regulated wastewater from the Pantex Plant represented 3,620 metric tons, 10 percent of the total hazardous waste generated by DOE. Similarly, mixed low-level wastewater from the TSCA Incinerator Facility at the Oak Ridge K-25 Site in Tennessee represented 55 percent of the total radioactive waste generated by DOE in 1992.

  8. INNOVATIVE FOSSIL FUEL FIRED VITRIFICATION TECHNOLOGY FOR SOIL REMEDIATION

    SciTech Connect (OSTI)

    J. Hnat; L.M. Bartone; M. Pineda

    2001-07-13

    This Summary Report summarizes the progress of Phases 3, 3A and 4 of a waste technology Demonstration Project sponsored under a DOE Environmental Management Research and Development Program and administered by the U.S. Department of Energy National Energy Technology Laboratory-Morgantown (DOE-NETL) for an ''Innovative Fossil Fuel Fired Vitrification Technology for Soil Remediation''. The Summary Reports for Phases 1 and 2 of the Program were previously submitted to DOE. The total scope of Phase 3 was to have included the design, construction and demonstration of Vortec's integrated waste pretreatment and vitrification process for the treatment of low level waste (LLW), TSCA/LLW and mixed low-level waste (MLLW). Due to funding limitations and delays in the project resulting from a law suit filed by an environmental activist and the extended time for DOE to complete an Environmental Assessment for the project, the scope of the project was reduced to completing the design, construction and testing of the front end of the process which consists of the Material Handling and Waste Conditioning (MH/C) Subsystem of the vitrification plant. Activities completed under Phases 3A and 4 addressed completion of the engineering, design and documentation of the Material Handling and Conditioning System such that final procurement of the remaining process assemblies can be completed and construction of a Limited Demonstration Project be initiated in the event DOE elects to proceed with the construction and demonstration testing of the MH/C Subsystem.

  9. Innovative vitrification for soil remediation

    SciTech Connect (OSTI)

    Jetta, N.W.; Patten, J.S.; Hnat, J.G. [Vortec Corp., Collegeville, PA (United States)] [and others

    1996-03-01

    The objective of this DOE demonstration program is to validate the performance and operation of the Vortec Cyclone Melting System (CMS{trademark}) for the processing of LLW contaminated soils found at DOE sites. This DOE vitrification demonstration project has successfully progressed through the first two phases. Phase 1 consisted of pilot scale testing with surrogate wastes and the conceptual design of a process plant operating at a generic DOE site. The objective of Phase 2, which is scheduled to be completed the end of FY 95, is to develop a definitive process plant design for the treatment of wastes at a specific DOE facility. During Phase 2, a site specific design was developed for the processing of LLW soils and muds containing TSCA organics and RCRA metal contaminants. Phase 3 will consist of a full scale demonstration at the DOE gaseous diffusion plant located in Paducah, KY. Several DOE sites were evaluated for potential application of the technology. Paducah was selected for the demonstration program because of their urgent waste remediation needs as well as their strong management and cost sharing financial support for the project.

  10. Innovative vitrification for soil remediation

    SciTech Connect (OSTI)

    Jetta, N.W.; Patten, J.S.; Hnat, J.G. [Vortec Corp., Collegeville, PA (United States)

    1995-10-01

    The objective of this DOE demonstration program is to validate the performance and operation of the Vortec Cyclone Melting System (CMS{trademark}) for the processing of LLW contaminated soils found at DOE sites. This DOE vitrification demonstration project has successfully progressed through the first two phases. Phase I consisted of pilot scale testing with surrogate wastes and the conceptual design of a process plant operating at a generic DOE site. The objective of Phase 2, which is scheduled to be completed the end of FY 95, is to develop a definitive process plant design for the treatment of wastes at a specific DOE facility. During Phase 2, a site specific design was developed for the processing of LLW soils and muds containing TSCA organics and RCRA metal contaminants. Phase 3 will consist of a full scale demonstration at the DOE gaseous diffusion plant located in Paducah, KY. Several DOE sites were evaluated for potential application of the technology. Paducah was selected for the demonstration program because of their urgent waste remediation needs as well as their strong management and cost sharing financial support for the project.

  11. Steam Reforming of Low-Level Mixed Waste

    SciTech Connect (OSTI)

    1998-01-01

    Under DOE Contract No. DE-AR21-95MC32091, Steam Reforming of Low-Level Mixed Waste, ThermoChem has successfully designed, fabricated and operated a nominal 90 pound per hour Process Development Unit (PDU) on various low-level mixed waste surrogates. The design construction, and testing of the PDU as well as performance and economic projections for a 500- lb/hr demonstration and commercial system are described. The overall system offers an environmentally safe, non-incinerating, cost-effective, and publicly acceptable method of processing LLMW. The steam-reforming technology was ranked the No. 1 non-incineration technology for destruction of hazardous organic wastes in a study commissioned by the Mixed Waste Focus Area published April 1997.1 The ThermoChem steam-reforming system has been developed over the last 13 years culminating in this successful test campaign on LLMW surrogates. Six surrogates were successfidly tested including a 750-hour test on material simulating a PCB- and Uranium- contaminated solid waste found at the Portsmouth Gaseous Diffusion Plant. The test results indicated essentially total (>99.9999oA) destruction of RCRA and TSCA hazardous halogenated organics, significant levels of volume reduction (> 400 to 1), and retention of radlonuclides in the volume-reduced solids. Cost studies have shown the steam-reforming system to be very cost competitive with more conventional and other emerging technologies.

  12. Steam reforming of low-level mixed waste. Final report

    SciTech Connect (OSTI)

    1998-06-01

    ThermoChem has successfully designed, fabricated and operated a nominal 90 pound per hour Process Development Unit (PDU) on various low-level mixed waste surrogates. The design, construction, and testing of the PDU as well as performance and economic projections for a 300-lb/hr demonstration and commercial system are described. The overall system offers an environmentally safe, non-incinerating, cost-effective, and publicly acceptable method of processing LLMW. The steam-reforming technology was ranked the No. 1 non-incineration technology for destruction of hazardous organic wastes in a study commissioned by the Mixed Waste Focus Area and published in April 1997. The ThermoChem steam-reforming system has been developed over the last 13 years culminating in this successful test campaign on LLMW surrogates. Six surrogates were successfully tested including a 750-hour test on material simulating a PCB- and Uranium-contaminated solid waste found at the Portsmouth Gaseous Diffusion Plant. The test results indicated essentially total (> 99.9999%) destruction of RCRA and TSCA hazardous halogenated organics, significant levels of volume reduction (> 400 to 1), and retention of radionuclides in the volume-reduced solids. Economic evaluations have shown the steam-reforming system to be very cost competitive with more conventional and other emerging technologies.

  13. H. R. 3467: a Bill to amend the Internal Revenue Code of 1954 to impose a surcharge tax on business activities to provide revenues for the trust fund known as the Hazardous Substance Response Superfund. Introduced in the House of Representatives, Ninety-Ninth Congress, First Session, October 1, 1985

    SciTech Connect (OSTI)

    Not Available

    1985-01-01

    The Superfund Revenue Act of 1985 (H.R. 3467) amends the Internal Revenue Code of 1954 and provides income for the Superfund trust fund by imposing a surcharge tax on business activities designated in section 4002 and varying with the number of employees. The bill also defines activities according to category of business and the degree of direct and indirect labor. Later sections describe the tax period and procedures for tax payments, exemptions for government entities and charitable organizations, neutrality for import and export goods, and other limitations and requirements involving payments to the trust fund.

  14. H. R. 1561: A bill to amend the Internal Revenue Code of 1986 to deny any deduction for certain oil and hazardous substance clean-up costs, introduced in the House of Representatives, One Hundred Second Congress, First Session, March 21, 1991

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    This bill would allow deductions for cleanup costs only if a taxpayer has a complete liability defense with respect to such discharges or qualifies for a liability limitation and is not liable for any punitive damages. The effective date of these amendments shall apply to discharges occurring on or after March 23, 1989.

  15. Detection of genotoxic and non-genotoxic carcinogens in Xpc{sup ?/?}p53{sup +/?} mice

    SciTech Connect (OSTI)

    Melis, Joost P.M.; Leiden University Medical Center, Department of Toxicogenetics, Leiden ; Speksnijder, Ewoud N.; Kuiper, Raoul V.; Dutch Molecular Pathology Center, Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht ; Salvatori, Daniela C.F.; Schaap, Mirjam M.; Leiden University Medical Center, Department of Toxicogenetics, Leiden ; Maas, Saskia; Robinson, Joke; Verhoef, Aart; Benthem, Jan van; Luijten, Mirjam; Steeg, Harry van

    2013-01-15

    An accurate assessment of the carcinogenic potential of chemicals and pharmaceutical drugs is essential to protect humans and the environment. Therefore, substances are extensively tested before they are marketed to the public. Currently, the rodent two-year bioassay is still routinely used to assess the carcinogenic potential of substances. However, over time it has become clear that this assay yields false positive results and also has several economic and ethical drawbacks including the use of large numbers of animals, the long duration, and the high cost. The need for a suitable alternative assay is therefore high. Previously, we have proposed the Xpa*p53 mouse model as a very suitable alternative to the two-year bioassay. We now show that the Xpc*p53 mouse model preserves all the beneficial traits of the Xpa*p53 model for sub-chronic carcinogen identification and can identify both genotoxic and non-genotoxic carcinogens. Moreover, Xpc*p53 mice appear to be more responsive than Xpa*p53 mice towards several genotoxic and non-genotoxic carcinogens. Furthermore, Xpc*p53 mice are far less sensitive than Xpa*p53 mice for the toxic activity of DNA damaging agents and as such clearly respond in a similar way as wild type mice do. These advantageous traits of the Xpc*p53 model make it a better alternative for in vivo carcinogen testing than Xpa*p53. This pilot study suggests that Xpc*p53 mice are suited for routine sub-chronic testing of both genotoxic and non-genotoxic carcinogens and as such represent a suitable alternative to possibly replace the murine life time cancer bioassay. Highlights: ? The Xpc*p53 mouse model is able to identify genotoxic and non-genotoxic carcinogens. ? Time, animals and cost can be significantly reduced compared to the 2-year bioassay. ? Xpc*p53 mice are more advantageous for carcinogen identification than Xpa*p53 mice. ? Xpc*p53 mice exhibit a wild type response upon exposure to genotoxicants.

  16. Paint for detection of radiological or chemical agents

    DOE Patents [OSTI]

    Farmer, Joseph C. (Tracy, CA); Brunk, James L. (Martinez, CA); Day, Sumner Daniel (Danville, CA)

    2010-08-24

    A paint that warns of radiological or chemical substances comprising a paint operatively connected to the surface, an indicator material carried by the paint that provides an indication of the radiological or chemical substances, and a thermo-activation material carried by the paint. In one embodiment, a method of warning of radiological or chemical substances comprising the steps of painting a surface with an indicator material, and monitoring the surface for indications of the radiological or chemical substances. In another embodiment, a paint is operatively connected to a vehicle and an indicator material is carried by the paint that provides an indication of the radiological or chemical substances.

  17. Method for warning of radiological and chemical agents using detection paints on a vehicle surface

    DOE Patents [OSTI]

    Farmer, Joseph C. (Tracy, CA); Brunk, James L. (Martinez, CA); Day, S. Daniel (Danville, CA)

    2012-03-27

    A paint that warns of radiological or chemical substances comprising a paint operatively connected to the surface, an indicator material carried by the paint that provides an indication of the radiological or chemical substances, and a thermo-activation material carried by the paint. In one embodiment, a method of warning of radiological or chemical substances comprising the steps of painting a surface with an indicator material, and monitoring the surface for indications of the radiological or chemical substances. In another embodiment, a paint is operatively connected to a vehicle and an indicator material is carried by the paint that provides an indication of the radiological or chemical substances.

  18. Aerial vehicle with paint for detection of radiological and chemical warfare agents

    DOE Patents [OSTI]

    Farmer, Joseph C.; Brunk, James L.; Day, S. Daniel

    2013-04-02

    A paint that warns of radiological or chemical substances comprising a paint operatively connected to the surface, an indicator material carried by the paint that provides an indication of the radiological or chemical substances, and a thermo-activation material carried by the paint. In one embodiment, a method of warning of radiological or chemical substances comprising the steps of painting a surface with an indicator material, and monitoring the surface for indications of the radiological or chemical substances. In another embodiment, a paint is operatively connected to a vehicle and an indicator material is carried by the paint that provides an indication of the radiological or chemical substances.

  19. How information resources are used by federal agencies in risk assessment applications

    SciTech Connect (OSTI)

    Legg, W.E.

    1990-12-31

    This paper discusses the structure and responsibilities of the US Army Toxic and Hazardous Materials Agency.

  20. Self-referencing spectrophotometric measurements

    DOE Patents [OSTI]

    O'Rourke, P.E.; Van Hare, D.R.

    1994-03-29

    A method is described for measuring the concentration of a chemical substance by spectrophotometry comprising the steps of placing a sample of a photoreactive substance between the light source and a spectrophotometer, obtaining an absorption spectrum of the substance using a fixed amount of light from the light source, obtaining a second absorption spectrum after a short interval, comparing the two to determine the concentration of the chemical substance from the difference in the spectra. If the chemical substance is not photoreactive, a photoreactive mixture can be made with a photoreactive dye that has photoreactive properties unique to the mixture. Alternatively, an optically transparent substrate can absorb the substance or the dye/substance mixture. 3 figures.