Sample records for toiyabe nf lessee

  1. A lessee's guide to leasing industrial equipment

    E-Print Network [OSTI]

    Johnson, Jones Eugene

    1959-01-01T23:59:59.000Z

    is included in the agree- ment, the lessee is treading on dangerous ground, The Internal Reve- nue Service will examine such agreements closely and may decide the original transaction was a sale and not a lease. Regardless, whether the lessee actually...A LESSEE'S GUIDE TO LEASING INDUSTRIAL EQUIPMENT A Thesis Submitted to the Graduate School of the Agricultural and Mechanical College of Texas in Partial fulfillment of the requirements for the degree of Master of Business Administration...

  2. Rate of return earned by lessees under cash bonus bidding for OCS oil and gas leases

    SciTech Connect (OSTI)

    Mead, W.J. (Univ. of California, Santa Barbara); Moseidjord, A.; Sorensen, P.E.

    1983-10-01T23:59:59.000Z

    An analysis of 1223 oil and gas leases issued between 1954 and 1969 reveals that lessees earned a 10.74% after-tax rate of return on equity on their lease investments. This is competitive with the average rate of return (11.8%) for all US manufacturing corporations over the 1954 to 1980 period. This record shows that firms bidding jointly pay more for their leases, obtain higher-quality leases, and earn higher rates of return of 11.74%. This indicates that bonus bidding tends to produce rational results since higher bonus bids are correlated with fewer dry holes and higher gross value of production or lease. The findings also show that wildcat leases yielded a 10.04% after-tax return compared to 14.59% for drainage leases, which is apparently due to the drainage lease owners having better information after working on adjacent leases. 12 references, 6 tables.

  3. USFS Humboldt-Toiyabe National Forest | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-fTri Global Energy LLC Place: Dallas,UGIURDBCOSO EGS PROJECT | Open

  4. NF Energy Saving Corp | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF Energy Saving Corp

  5. NF-?B Fans the Flames of Lung Carcinogenesis

    E-Print Network [OSTI]

    Jacks, Tyler E.

    This perspective on Deng et al. (beginning on p. 424 in this issue of the journal) examines the link between NF-?B and lung tumorigenesis. Experiments in genetically engineered mouse models of lung cancers are elucidating ...

  6. Universality of the Nf=2 Running Coupling

    E-Print Network [OSTI]

    K. Murano; S. Aoki; S. Takeda; Y. Taniguchi

    2009-03-06T23:59:59.000Z

    We investigate universality of the Nf=2 running coupling in the Sch\\"odinger functional scheme, by calculating the step scaling function in lattice QCD with the renorm alization group (RG) improved gauge action at both weak(u=0.9796) and strong(u=3.3340) couplings, where u=\\bar{g}^2_SF with \\bar{g}_SF being the running coupling in this scheme. In our main calculations, we use the tree-level value for O(a) improvement coefficients of boundary gauge fields. In addition we employ the 1-loop value for them in order to see how scaling behaviours are affected by them. In the continuum limit, the step scaling function obtained from the RG improved gauge actions agrees with the previous result obtained from the plaquette action within errors at both couplings, though errors of our result are larger. Combined fits using all data with the RG improved action as well as the plaquette action reduce errors in the continuum limit by 2% at the weak coupling and 22% at the strong coupling.

  7. Haploid reporter screens aimed at identification of NF-kappaB regulators

    E-Print Network [OSTI]

    Lee, Clarissa Ci

    2014-01-01T23:59:59.000Z

    Nuclear factor-kappaB (NF-KB) is a family of transcription factors that are essential for execution of both the innate and the adaptive immune response. NF-KB regulates hundreds of genes involved in critical processes such ...

  8. Porcine arterivirus activates the NF-{kappa}B pathway through I{kappa}B degradation

    SciTech Connect (OSTI)

    Lee, Sang-Myeong [Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri-Columbia, MO 65211 (United States); Kleiboeker, Steven B. [Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri-Columbia, MO 65211 (United States) and Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, MO 65211 (United States)]. E-mail: KleiboekerS@Missouri.edu

    2005-11-10T23:59:59.000Z

    Nuclear factor-kappaB (NF-{kappa}B) is a critical regulator of innate and adaptive immune function as well as cell proliferation and survival. The present study demonstrated for the first time that a virus belonging to the Arteriviridae family activates NF-{kappa}B in MARC-145 cells and alveolar macrophages. In porcine reproductive and respiratory syndrome virus (PRRSV)-infected cells, NF-{kappa}B activation was characterized by translocation of NF-{kappa}B from the cytoplasm to the nucleus, increased DNA binding activity, and NF-{kappa}B-regulated gene expression. NF-{kappa}B activation was increased as PRRSV infection progressed and in a viral dose-dependent manner. UV-inactivation of PRRSV significantly reduced the level of NF-{kappa}B activation. Degradation of I{kappa}B protein was detected late in PRRSV infection, and overexpression of the dominant negative form of I{kappa}B{alpha} (I{kappa}B{alpha}DN) significantly suppressed NF-{kappa}B activation induced by PRRSV. However, I{kappa}B{alpha}DN did not affect viral replication and viral cytopathic effect. PRRSV infection induced oxidative stress in cells by generating reactive oxygen species (ROS), and antioxidants inhibited NF-{kappa}B DNA binding activity in PRRSV-infected cells, suggesting ROS as a mechanism by which NF-{kappa}B was activated by PRRSV infection. Moreover, NF-{kappa}B-dependent expression of matrix metalloproteinase (MMP)-2 and MMP-9 was observed in PRRSV-infected cells, an observation which implies that NF-{kappa}B activation is a biologically significant aspect of PRRSV pathogenesis. The results presented here provide a basis for understanding molecular pathways of pathology and immune evasion associated with disease caused by PRRSV.

  9. Smoothing single-crystalline SiC surfaces by reactive ion etching using pure NF{sub 3} and NF{sub 3}/Ar mixture gas plasmas

    SciTech Connect (OSTI)

    Tasaka, Akimasa, E-mail: aki-tasaka-load@yahoo.co.jp [Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, 1-3 Miyako-dani, Tatara, Kyotanabe, Kyoto 610-0321, Japan and Department of Applied Chemistry, Graduate School of Engineering, Doshisha University, 1-3 Miyako-dani, Tatara, Kyotanabe, Kyoto 610-0321 (Japan); Kotaka, Yuki [Department of Applied Chemistry, Graduate School of Engineering, Doshisha University, 1-3 Miyako-dani, Tatara, Kyotanabe, Kyoto 610-0321 (Japan); Oda, Atsushi; Saito, Morihiro [Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, 1-3 Miyako-dani, Tatara, Kyotanabe, Kyoto 610-0321 (Japan); Tojo, Tetsuro [Toyo Tanso Co, Ltd., 5-7-2 Takeshima, Nishi yodogawa-ku, Osaka 555-0011 (Japan); Inaba, Minoru [Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, 1-3 Miyako-dani, Tatara, Kyotanabe, Kyoto 610-0321, Japan and Department of Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, 1-3 Miyako-dani, Tatara, Kyotanabe, Kyoto 610-0321 (Japan)

    2014-09-01T23:59:59.000Z

    In pure NF{sub 3} plasma, the etching rates of four kinds of single-crystalline SiC wafer etched at NF{sub 3} pressure of 2?Pa were the highest and it decreased with an increase in NF{sub 3} pressure. On the other hand, they increased with an increase in radio frequency (RF) power and were the highest at RF power of 200?W. A smooth surface was obtained on the single-crystalline 4H-SiC after reactive ion etching at NF{sub 3}/Ar gas pressure of 2?Pa and addition of Ar to NF{sub 3} plasma increased the smoothness of SiC surface. Scanning electron microscopy observation revealed that the number of pillars decreased with an increase in the Ar-concentration in the NF{sub 3}/Ar mixture gas. The roughness factor (R{sub a}) values were decreased from 51.5?nm to 25.5?nm for the As-cut SiC, from 0.25?nm to 0.20?nm for the Epi-SiC, from 5.0?nm to 0.7?nm for the Si-face mirror-polished SiC, and from 0.20?nm to 0.16?nm for the C-face mirror-polished SiC by adding 60% Ar to the NF{sub 3} gas. Both the R{sub a} values of the Epi- and the C-face mirror-polished wafer surfaces etched using the NF{sub 3}/Ar (40:60) plasma were similar to that treated with mirror polishing, so-called the Catalyst-Referred Etching (CARE) method, with which the lowest roughness of surface was obtained among the chemical mirror polishing methods. Etching duration for smoothing the single-crystalline SiC surface using its treatment was one third of that with the CARE method.

  10. 1 Managed by UT-Battelle for the U.S. Department of Energy NF Splash Mitigation 12 July 2011

    E-Print Network [OSTI]

    McDonald, Kirk

    1 Managed by UT-Battelle for the U.S. Department of Energy NF Splash Mitigation 12 July 2011 by UT-Battelle for the U.S. Department of Energy NF Splash Mitigation 12 July 2011 Splash Mitigation for comparison & final determination #12;3 Managed by UT-Battelle for the U.S. Department of Energy NF Splash

  11. IKK{epsilon} modulates RSV-induced NF-{kappa}B-dependent gene transcription

    SciTech Connect (OSTI)

    Bao Xiaoyong; Indukuri, Hemalatha; Liu Tianshuang; Liao Suiling [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Tian, Bing; Brasier, Allan R. [Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX (United States); Garofalo, Roberto P. [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX (United States); Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX (United States); Casola, Antonella, E-mail: ancasola@utmb.ed [Department of Pediatrics, University of Texas Medical Branch, Galveston, TX (United States); Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX (United States); Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX (United States)

    2010-12-20T23:59:59.000Z

    Respiratory syncytial virus (RSV), a negative-strand RNA virus, is the most common cause of epidemic respiratory disease in infants and young children. RSV infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including Nuclear Factor-{kappa}B (NF-{kappa}B). In this study we have investigated the role of the non canonical I{kappa}B kinase (IKK){epsilon} in modulating RSV-induced NF-{kappa}B activation. Our results show that inhibition of IKK{epsilon} activation results in significant impairment of viral-induced NF-{kappa}B-dependent gene expression, through a reduction in NF-{kappa}B transcriptional activity, without changes in nuclear translocation or DNA-binding activity. Absence of IKK{epsilon} results in a significant decrease of RSV-induced NF-{kappa}B phosphorylation on serine 536, a post-translational modification important for RSV-induced NF-{kappa}B-dependent gene expression, known to regulate NF-{kappa}B transcriptional activity without affecting nuclear translocation. This study identifies a novel mechanism by which IKK{epsilon} regulates viral-induced cellular signaling.

  12. Homeostatic control of IkappaB metabolism determines NF- kappaB responsiveness

    E-Print Network [OSTI]

    O'Dea, Ellen Louise

    2010-01-01T23:59:59.000Z

    19-26. O'Dea, E. and Hoffmann, A. 2009. NF-kappaB signaling.J.D. , Levchenko, A. , and Hoffmann, A. 2007. A homeostaticS.L. , Cheng, C. , and Hoffmann, A. 2007. A temporal code

  13. Response and Resistance to NF-?B Inhibitors in Mouse Models of Lung Adenocarcinoma

    E-Print Network [OSTI]

    Xue, Wen

    Lung adenocarcinoma is a leading cause of cancer death worldwide. We recently showed that genetic inhibition of the NF-?B pathway affects both the initiation and the maintenance of lung cancer, identifying this pathway as ...

  14. Requirement for NF-?B signalling in a mouse model of lung adenocarcinoma

    E-Print Network [OSTI]

    Meylan, Etienne

    NF-?B transcription factors function as crucial regulators of inflammatory and immune responses as well as of cell survival. They have also been implicated in cellular transformation and tumorigenesis. However, despite ...

  15. A test of first order scaling in Nf=2 QCD

    E-Print Network [OSTI]

    G. Cossu; M. D'Elia; A. Di Giacomo; C. Pica

    2007-09-30T23:59:59.000Z

    We complete our analysis of Nf=2 QCD based on the lattice staggered fermion formulation. Using a series of Monte Carlo simulations at fixed (amq*Ls^yh) one is able to test the universality class with given critical exponent yh. This strategy has been used to test the O(4) universality class and it has been presented at the previous Lattice conferences. No agreement was found with simulations in the mass range amq=[0.01335,0.15] using lattices with Ls=16 up to 32 and Lt=4. With the same strategy, we now investigate the possibility of a first order transition using a new set of Monte Carlo data corresponding to yh=3 in the same mass and volume range as the one used for O(4). A substantial agreement is observed both in the specific heat scaling and in the scaling of the chiral condensate, while the chiral susceptibilities still presents visible deviation from scaling in the mass range explored.

  16. Sci. Aging Knowl. Environ., Vol. 2004, Issue 34, pp. nf78, 25 August 2004 [DOI: 10.1126/sageke.2004.34.nf78

    E-Print Network [OSTI]

    Seroude, Laurent

    : They scrubbed fly eggs with alcohol, fed the animals irradiated food, and kept them in sterile containers: http://sageke.sciencemag.org/cgi/content/full/2004/34/nf78 Nasty germs in fast-food burgers can kill direction--from fare that included antibiotics to "dirty" food--extended fly life on day 2 but not on day 4

  17. NF-?B1 Inhibits TLR-Induced IFN-? Production in Macrophages Through TPL-2-dependent ERK Activation

    E-Print Network [OSTI]

    Yang, Huei-Ting

    Although NF-?B1 p50/p105 has critical roles in immunity, the mechanism by which NF-?B1 regulates inflammatory responses is unclear. In this study, we analyzed the gene expression profile of LPS-stimulated Nfkb1?/? macrophages ...

  18. XIAP and cIAP1 amplifications induce Beclin 1-dependent autophagy through NF?B activation

    E-Print Network [OSTI]

    Lin, Fang; Ghislat, Ghita; Luo, Shouqing; Renna, Maurizio; Siddiqi, Farah; Rubinsztein, David C.

    2015-02-10T23:59:59.000Z

    ). In both steady state and stimulated conditions with TGF? that activates NF?B through TAK1 (33), nei- ther the overexpression of XIAP nor its knockdown affected the transcriptional activation of the NF?B promoter (Fig. 3B, top). The effect of TGF?...

  19. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07T23:59:59.000Z

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  20. Instruction sheet for the consolidation of the standard NF EN 693 dated September 2001 and its amendment A1 Machine tools : Safety : Hydraulic presses

    E-Print Network [OSTI]

    Association Franaise de Normalisation. Paris

    2009-01-01T23:59:59.000Z

    Instruction sheet for the consolidation of the standard NF EN 693 dated September 2001 and its amendment A1

  1. 1 Managed by UT-Battelle for the U.S. Department of Energy NF-IDS Videoconference 2 Jun 2009

    E-Print Network [OSTI]

    McDonald, Kirk

    1 Managed by UT-Battelle for the U.S. Department of Energy NF-IDS Videoconference 2 Jun 2009 by UT-Battelle for the U.S. Department of Energy NF-IDS Videoconference 2 Jun 2009 Purpose Compare Compilation of magnet geometry data #12;3 Managed by UT-Battelle for the U.S. Department of Energy NF

  2. Introduction The NF-B/Rel family of transcription factors is composed of

    E-Print Network [OSTI]

    Arnold, Jonathan

    gene expression exhibited a requirement for the host IKK complex. A detailed kinetic analysis of NF- B expression of anti-apoptotic genes and prevent death of infected cells in order to allow replication in a loss of the anti-apoptotic phenotype and a decrease in pro-survival gene expression (Molestina et al

  3. Single-cell NF-kB dynamics reveal digital activation and analogue information processing

    E-Print Network [OSTI]

    Quake, Stephen R.

    LETTERS Single-cell NF-kB dynamics reveal digital activation and analogue information processing and is a digital process at the single-cell level with fewer cells responding at lower doses. Cells also encode that reproduces both the digital and analogue dynamics as well as most gene expression profiles at all measured

  4. Microstructural Evolution in Two Variants of NF709 at 1023 and 1073 K

    E-Print Network [OSTI]

    Cambridge, University of

    and that could invalidate the extrapolations of short-term creep test data, and to identify any precipitates.K.D.H. BHADESHIA The microstructural state of two grades of the creep-resistant austenitic stainless steels NF709 regarded as one of the best austenitic steels for elevated temperature applications because of its creep

  5. Title 43 CFR 3202 Lessee Qualifications | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f <MaintainedInformationThePty LtdOpen EnergyAct | OpenQualifications

  6. Coal dust contiguity-induced changes in the concentration of TNF- and NF- B p65 on the ocular surface

    SciTech Connect (OSTI)

    Sun, Z.Y.; Hong, J.; Liu, Z.Y.; Jin, X.D.; Gu, C.H. [China Medical University, Shenyang (China)

    2009-07-01T23:59:59.000Z

    To observe the influence of coal dust on ocular surface of coal miners and rabbits with coal dust contiguity on expression TNF- and NF- Bp65 and dry eye occurrence. Expression TNF- and NF- Bp65 in ocular surface were determined. Results showed tear production, BUT and lysozyme decreased for coal miners and rabbits with coal dust contiguity. Coal dust exposure was linked to development of xerophthalmia, and induced a higher expression of NF- B p65 and TNF- perhaps as a mechanism to resist coal dust ocular surface injury.

  7. Omentin inhibits TNF-{alpha}-induced expression of adhesion molecules in endothelial cells via ERK/NF-{kappa}B pathway

    SciTech Connect (OSTI)

    Zhong, Xia, E-mail: zhongxia1977@126.com [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China); Li, Xiaonan; Liu, Fuli; Tan, Hui [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China); Shang, Deya, E-mail: wenhuashenghuo1@163.com [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)

    2012-08-24T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Omentin inhibited TNF-{alpha}-induced adhesion of THP-1 cells to HUVECs. Black-Right-Pointing-Pointer Omentin reduces expression of ICAM-1 and VCAM-1 induced by TNF-{alpha} in HUVECs. Black-Right-Pointing-Pointer Omentin inhibits TNF-{alpha}-induced ERK and NF-{kappa}B activation in HUVECs. Black-Right-Pointing-Pointer Omentin supreeses TNF-{alpha}-induced expression of ICAM-1 and VCAM-1 via ERK/NF-{kappa}B pathway. -- Abstract: In the present study, we investigated whether omentin affected the expression of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in tumor necrosis factor-{alpha} (TNF-{alpha}) induced human umbilical vein endothelial cells (HUVECs). Our data showed that omentin decreased TNF-{alpha}-induced expression of ICAM-1 and VCAM-1 in HUVECs. In addition, omentin inhibited TNF-{alpha}-induced adhesion of THP-1 cells to HUVECs. Further, we found that omentin inhibited TNF-{alpha}-activated signal pathway of nuclear factor-{kappa}B (NF-{kappa}B) by preventing NF-{kappa}B inhibitory protein (I{kappa}B{alpha}) degradation and NF-{kappa}B/DNA binding activity. Omentin pretreatment significantly inhibited TNF-{alpha}-induced ERK activity and ERK phosphorylation in HUVECs. Pretreatment with PD98059 suppressed TNF-{alpha}-induced NF-{kappa}B activity. Omentin, NF-kB inhibitor (BAY11-7082) and ERK inhibitor (PD98059) reduced the up-regulation of ICAM-1 and VCAM-1 induced by TNF-{alpha}. These results suggest that omentin may inhibit TNF-{alpha}-induced expression of adhesion molecules in endothelial cells via blocking ERK/NF-{kappa}B pathway.

  8. $Re(A_0)$, $Re(A_2)$ and RG evolution for $N_f=3$

    E-Print Network [OSTI]

    Choi, K; Choi, Keunsu; Lee, Weonjong

    2005-01-01T23:59:59.000Z

    We present results of $Re (A_0)$ and $Re (A_2)$ calculated using HYP staggered fermions on the lattice of $16^3 \\times 64$ at $\\beta=6.0$. These results are obtained using leading order chiral perturbation in quenched QCD. Buras's original RG evolution matrix develops a removable singularity for $N_f=3$. This subtlety is resolved by finding a finite solution to RG equation and the results are presented.

  9. Agreement Execution Process Study: CRADAs and NF-WFO Agreements and the Speed of Business

    SciTech Connect (OSTI)

    Harrer, Bruce J.; Cejka, Cheryl L.; Macklin, Richard; Miksovic, Ann

    2011-02-01T23:59:59.000Z

    This report summarizes the findings of a study on the execution of Cooperative Research and Development Agreements (CRADAs) and Non-Federal Work for Others (NF-WFO) agreements across the U.S. Department of Energy (DOE) laboratory complex. The study provides quantitiative estimates of times required to negotiate and execute these agreements across the DOE complex. It identifies factors impacting on cycle times and describes best practicies used at various laboratories and site offices that reduce cycle times.

  10. NF-{kappa}B suppresses HIF-1{alpha} response by competing for P300 binding

    SciTech Connect (OSTI)

    Mendonca, Daniela B.S., E-mail: daniela_mendonca@dentistry.unc.edu [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil); Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 330 Brauer Hall, CB 7450, Chapel Hill, NC 27599 (United States); Mendonca, Gustavo [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil) [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil); Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 330 Brauer Hall, CB 7450, Chapel Hill, NC 27599 (United States); Aragao, Francisco J.L. [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil) [Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e Biotecnologia, SGAN Quadra 916, Av. W5 Norte, 70790-160 Brasilia, DF (Brazil); Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Introducao e Expressao de Genes, PqEB W5 Norte, 70770-900 Brasilia, DF (Brazil); Cooper, Lyndon F., E-mail: lyndon_cooper@dentistry.unc.edu [Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 330 Brauer Hall, CB 7450, Chapel Hill, NC 27599 (United States)

    2011-01-28T23:59:59.000Z

    Research highlights: {yields} p65 completely blocked HIF-1{alpha} activity at the HRE on different cell lines. {yields} p65 caused minor changes in HIF-1{alpha} and HIF-1{alpha} target genes mRNA expression. {yields} p65 reduced transcription of VEGF promoter. {yields} p65 competes with HIF-1{alpha} for p300. -- Abstract: Hypoxia has emerged as a key determinant of osteogenesis. HIF-1{alpha} is the transcription factor mediating hypoxia responses that include induction of VEGF and related bone induction. Inflammatory signals antagonize bone repair via the NF-{kappa}B pathway. The present investigation explored the functional relationship of hypoxia (HIF-1{alpha} function) and inflammatory signaling (NF-{kappa}B) in stem like and osteoprogenitor cell lines. The potential interaction between HIF-1{alpha} and NF-{kappa}B signaling was explored by co-transfection studies in hFOB with p65, HIF-1{alpha} and 9x-HRE-luc or HIF-1{alpha} target genes reporter plasmids. Nuclear cross-talk was directly tested using the mammalian Gal4/VP16 two-hybrid, and confirmed by co-immunoprecipitation/western blotting assays. The results show that inflammatory stimulation (TNF-{alpha} treatment) causes a marked inhibition of HIF-1{alpha} function at the HRE in all cell lines studied. Also, co-transfection with p65 expression vector leads to reduced hVEGFp transcription after DFO-induced hypoxia. However, TNF-{alpha} treatment had little effect on HIF-1{alpha} mRNA levels. The functional interaction of Gal4-HIF-1{alpha} and VP16-p300 fusion proteins is effectively blocked by expression of p65 in a dose dependent manner. It was concluded that NF-{kappa}B-mediated inflammatory signaling is able to block HIF-1{alpha} transactivation at HRE-encoding genes by direct competition for p300 binding at the promoter. Inflammation may influence the stem cell niche and tissue regeneration by influencing cellular responses to hypoxia.

  11. IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh School footballHydrogen andHypernuclei in Hall CInbydoeCELLIDRMSIDS-NF

  12. FY13 Summary Report on the Augmentation of the Spent Fuel Composition Dataset for Nuclear Forensics: SFCOMPO/NF

    SciTech Connect (OSTI)

    Brady Raap, Michaele C.; Lyons, Jennifer A.; Collins, Brian A.; Livingston, James V.

    2014-03-31T23:59:59.000Z

    This report documents the FY13 efforts to enhance a dataset of spent nuclear fuel isotopic composition data for use in developing intrinsic signatures for nuclear forensics. A review and collection of data from the open literature was performed in FY10. In FY11, the Spent Fuel COMPOsition (SFCOMPO) excel-based dataset for nuclear forensics (NF), SFCOMPO/NF was established and measured data for graphite production reactors, Boiling Water Reactors (BWRs) and Pressurized Water Reactors (PWRs) were added to the dataset and expanded to include a consistent set of data simulated by calculations. A test was performed to determine whether the SFCOMPO/NF dataset will be useful for the analysis and identification of reactor types from isotopic ratios observed in interdicted samples.

  13. ABD56 causes osteoclast apoptosis by inhibiting the NF{kappa}B and ERK pathways

    SciTech Connect (OSTI)

    Idris, Aymen [Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh, EH4 2XU (United Kingdom); Mrak, Emanuela [Bone Metabolic Unit, Scientific Institute H San Raffaele, via Olgettina 60, 20132 Milano (Italy); Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milano, via Vanvitelli 32, 20129 Milano (Italy); Greig, Iain [Department of Medicine and Therapeutics, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD (United Kingdom); Guidobono, Francesca [Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milano, via Vanvitelli 32, 20129 Milano (Italy); Ralston, Stuart H. [Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh, EH4 2XU (United Kingdom); Hof, Rob van 't [Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh, EH4 2XU (United Kingdom)], E-mail: rvanthof@staffmail.ed.ac.uk

    2008-06-20T23:59:59.000Z

    We have previously shown that the biphenylcarboxylic acid butanediol ester (ABD56) inhibits osteoclast formation and activity in vitro and in vivo. However, the mechanism of action of this compound is unknown. ABD56 inhibited osteoclast formation and caused osteoclast apoptosis, but had no effects on osteoblasts or macrophages. As the NF{kappa}B and MAPK pathways are essential for osteoclast formation and survival, we studied the effects of ABD56 on these pathways. ABD56 caused phosphorylation of p38, JNK and nuclear translocation of c-jun in osteoclasts. ABD56-induced apoptosis was prevented by the caspase inhibitor zVAD-fmk but was not prevented by the p38- or JNK-inhibitors. ABD56 completely abolished RANKL-induced I{kappa}B and ERK1/2 phosphorylation. Increasing the amount of RANKL partially rescued ABD56-induced apoptosis, indicating that the apoptosis is most probably due to the inhibition of survival signals such as ERK and NF{kappa}B, rather than activation of the p38 or Jnk MAPK pathways.

  14. Lipid metabolism enzyme 5-LOX and its metabolite LTB4 are capable of activating transcription factor NF-{kappa}B in hepatoma cells

    SciTech Connect (OSTI)

    Zhao, Yu [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China); Wang, Wenhui [Department of Interventional Radiography, First Hospital of Lanzhou University, No.1 Donggang West Road, Lanzhou, Gansu Province 730000 (China)] [Department of Interventional Radiography, First Hospital of Lanzhou University, No.1 Donggang West Road, Lanzhou, Gansu Province 730000 (China); Wang, Qi [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China); Ye, Lihong, E-mail: yelihong@nankai.edu.cn [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071 (China)

    2012-02-24T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer 5-LOX is able to upregulate expression of NF-{kappa}B p65. Black-Right-Pointing-Pointer 5-LOX enhances nuclear translocation of NF-{kappa}B p65 via increasing p-I{kappa}B-{alpha} level. Black-Right-Pointing-Pointer 5-LOX stimulates transcriptional activity of NF-{kappa}B in hepatoma cells. Black-Right-Pointing-Pointer LTB4 activates transcriptional activity of NF-{kappa}B in hepatoma cells. -- Abstract: The issue that lipid metabolism enzyme and its metabolites regulate transcription factors in cancer cell is not fully understood. In this study, we first report that the lipid metabolism enzyme 5-Lipoxygenase (5-LOX) and its metabolite leukotriene B4 (LTB4) are capable of activating nuclear factor-{kappa}B (NF-{kappa}B) in hepatoma cells. We found that the treatment of MK886 (an inhibitor of 5-LOX) or knockdown of 5-LOX was able to downregulate the expression of NF-{kappa}B p65 at the mRNA level and decreased the phosphorylation level of inhibitor {kappa}B{alpha} (I{kappa}B{alpha}) in the cytoplasm of hepatoma HepG2 or H7402 cells, which resulted in the decrease of the level of nuclear NF-{kappa}B p65. These were confirmed by immunofluorescence staining in HepG2 cell. Moreover, the above treatments were able to decrease the transcriptional activity of NF-{kappa}B in the cells. The LTB4, one of metabolites of 5-LOX, is responsible for 5-LOX-activated NF-{kappa}B in a dose-dependent manner. Thus, we conclude that the lipid metabolism enzyme 5-LOX and its metabolite LTB4 are capable of activating transcription factor NF-{kappa}B in hepatoma cells. Our finding provides new insight into the significance of lipid metabolism in activation of transcription factors in cancer.

  15. mm-Wave Op-Amps employing simple-Miller compensation, with OIP3/PDC ratios of 211 (10 dB NF) and 144 (6.0 dB NF) at 2 GHz

    E-Print Network [OSTI]

    Rodwell, Mark J. W.

    mm-Wave Op-Amps employing simple-Miller compensation, with OIP3/PDC ratios of 211 (10 dB NF/PDC ratio at fs = 2-3 GHz, compared to state-of-the-art. We address considerations important 1000 mW power dissipation PDC . This very high ratio of output-referred third-order-intercept power

  16. Modeling green fluorescent protein transcription, translation and modification as a method to obtain NF-kappaB activation profiles

    E-Print Network [OSTI]

    Laible, Allyson Marie

    2009-05-15T23:59:59.000Z

    that fluorescence increases up to 24 hours after an initial delay of approximately four hours. The fluorescence data was also used to develop a model describing significant events leading to NF-?B activation and GFP expression. In addition, a model describing...

  17. TCR and CD28 activate the transcription factor NF- B in T-cells viadistinct adaptor signaling complexes

    E-Print Network [OSTI]

    Thaker, Youg Raj; Schneider, Helga; Rudd, Christopher E.

    2014-10-23T23:59:59.000Z

    of NF-{kappa}B activation in T cells via association of the adapter proteins ADAP and CARMA1. Science 2007;316:7548. [30] Sommer K, Guo B, Pomerantz JL, Bandaranayake AD, Moreno-Garca ME, Ovechkina YL, et al. Phosphorylation of the CARMA1 linker...

  18. Enhancement of CD8+ T-cell memory by removal of a vaccinia virus NF-?B inhibitor

    E-Print Network [OSTI]

    Ren, Hongwei; Ferguson, Brian J.; de Motes, Carlos Maluquer; Sumner, Rebecca P.; Harman, Laura; Smith, Geoffrey L.

    2014-11-07T23:59:59.000Z

    induction of NF-kappaB activation via an ERK2 pathway in virus-infected human embryonic kidney cells. J Virol 2006;80(17):8676-85. 63. Harte MT, Haga IR, Maloney G, et al. The poxvirus protein A52R targets Toll-like receptor signaling complexes...

  19. Mast cell chymase induces smooth muscle cell apoptosis by disrupting NF-{kappa}B-mediated survival signaling

    SciTech Connect (OSTI)

    Leskinen, Markus J. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Heikkilae, Hanna M. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Speer, Mei Y. [Bioengineering Department, University of Washington, Seattle, WA 98195 (United States); Hakala, Jukka K. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Laine, Mika [Department of Medicine, Helsinki University Central Hospital and Minerva Institute for Medical Research, Helsinki (Finland); Kovanen, Petri T. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Lindstedt, Ken A. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland)]. E-mail: ken.lindstedt@wri.fi

    2006-05-01T23:59:59.000Z

    Chymase released from activated mast cells induces apoptosis of vascular smooth muscle cells (SMCs) in vitro by degrading the pericellular matrix component fibronectin, so causing disruption of focal adhesion complexes and Akt dephosphorylation, which are necessary for cell adhesion and survival. However, the molecular mechanisms of chymase-mediated apoptosis downstream of Akt have remained elusive. Here, we show by means of RT-PCR, Western blotting, EMSA, immunocytochemistry and confocal microscopy, that chymase induces SMC apoptosis by disrupting NF-{kappa}B-mediated survival signaling. Following chymase treatment, the translocation of active NF-{kappa}B/p65 to the nucleus was partly abolished and the amount of nuclear p65 was reduced. Pretreatment of SMCs with chymase also inhibited LPS- and IL-1{beta}-induced nuclear translocation of p65. The chymase-induced degradation of p65 was mediated by active caspases. Loss of NF-{kappa}B-mediated transactivation resulted in downregulation of bcl-2 mRNA and protein expression, leading to mitochondrial swelling and release of cytochrome c. The apoptotic process involved activation of both caspase 9 and caspase 8. The results reveal that, by disrupting the NF-{kappa}B-mediated survival-signaling pathway, activated chymase-secreting mast cells can mediate apoptosis of cultured arterial SMCs. Since activated mast cells colocalize with apoptotic SMCs in vulnerable areas of human atherosclerotic plaques, they may participate in the weakening and rupture of atherosclerotic plaques.

  20. Journal of Membrane Science 256 (2005) 134142 Characterization of novel acid-stable NF membranes before and after

    E-Print Network [OSTI]

    Freger, Viatcheslav "Slava"

    2005-01-01T23:59:59.000Z

    to hot 20% sulfuric acid solutions for various times. Swelling and morphological changes measured Nanofiltration (NF) membranes represent a class of mem- branes with capabilities to selectively separate low- sulfone, which support an ultra-thin selective layer a few tens to a few hundred of nanometers thick

  1. D-Dbar Mixing in the Standard Model and Beyond from Nf=2 Twisted Mass QCD

    E-Print Network [OSTI]

    N. Carrasco; M. Ciuchini; P. Dimopoulos; R. Frezzotti; V. Gimenez; V. Lubicz; G. C. Rossi; F. Sanfilippo; L. Silvestrini; S. Simula; C. Tarantino

    2014-06-13T23:59:59.000Z

    We present the first unquenched lattice QCD results for the bag parameters controlling the short distance contribution to D meson oscillations in the Standard Model and beyond. We have used the gauge configurations produced by the European Twisted Mass Collaboration with Nf = 2 dynamical quarks, at four lattice spacings and light meson masses in the range 280-500 MeV. Renormalization is carried out non-perturbatively with the RI-MOM method. The bag-parameter results have been used to constrain New Physics effects in D-Dbar mixing, to put a lower bound to the generic New Physics scale and to constrain off-diagonal squark mass terms for TeV-scale Supersymmetry.

  2. Kaon semileptonic vector form factor with $N_f=2+1+1$ Twisted Mass fermions

    E-Print Network [OSTI]

    Nuria Carrasco; Paolo Lami; Vittorio Lubicz; Eleonora Picca; Lorenzo Riggio; Silvano Simula; Cecilia Tarantino

    2014-10-27T23:59:59.000Z

    We investigate the vector form factor relevant for the $K_{\\ell 3}$ semileptonic decay using maximally twisted-mass fermions with 4 dynamical flavours ($N_f=2+1+1$). Our simulations feature pion masses ranging from $210$ MeV to approximately $450$ MeV and lattice spacing values as small as $0.06$fm. Our main result for the vector form factor at zero 4-momentum transfer is $f_+(0)=0.9683(65)$ where the uncertainty is both statistical and systematic. By combining our result with the experimental value of $f_+(0)|V_{us}|$ we obtain $|V_{us}|=0.2234(16)$, which satisfies the unitarity constraint of the Standard Model at the permille level.

  3. Ascorbic acid suppresses endotoxemia and NF-?B signaling cascade in alcoholic liver fibrosis in guinea pigs: A mechanistic approach

    SciTech Connect (OSTI)

    Abhilash, P.A.; Harikrishnan, R.; Indira, M., E-mail: indiramadambath@gmail.com

    2014-01-15T23:59:59.000Z

    Alcohol consumption increases the small intestinal bacterial overgrowth (SIBO) and intestinal permeability of endotoxin. The endotoxin mediated inflammatory signaling plays a major role in alcoholic liver fibrosis. We evaluated the effect of ascorbic acid (AA), silymarin and alcohol abstention on the alcohol induced endotoxemia and NF-?B activation cascade pathway in guinea pigs (Cavia porcellus). Guinea pigs were administered ethanol at a daily dose of 4 g/kg b.wt for 90 days. After 90 days, ethanol administration was stopped. The ethanol treated animals were divided into abstention, silymarin (250 mg/kg b.wt) and AA (250 mg/kg b.wt) supplemented groups and maintained for 30 days. The SIBO, intestinal permeability and endotoxin were significantly increased in the ethanol group. The mRNA expressions of intestinal proteins claudin, occludin and zona occludens-1 were significantly decreased in ethanol group. The mRNA levels of inflammatory receptors, activity of IKK? and the protein expressions of phospho-I?B?, NF-?B, TNF-?, TGF-?{sub 1} and IL-6 were also altered in ethanol group. The expressions of fibrosis markers ?-SMA, ?{sub 1} (I) collagen and sirius red staining in the liver revealed the induction of fibrosis. But the supplementation of AA could induce greater reduction of ethanol induced SIBO, intestinal barrier defects, NF-?B activation and liver fibrosis than silymarin. The possible mechanism may be the inhibitory effect of AA on SIBO, intestinal barrier defect and IKK?, which decreased the activation of NF-?B and synthesis of cytokines. This might have led to suppression of HSCs activation and liver fibrosis. - Highlights: Alcohol increases intestinal bacterial overgrowth and permeability of endotoxin. Endotoxin mediated inflammation plays a major role in alcoholic liver fibrosis. Ascorbic acid reduces endotoxemia, NF-?B activation and proinflammatory cytokines. AA's action is by inhibition of SIBO, IKK? and alteration of intestinal permeability. This might have led to suppression of HSCs activation and liver fibrosis.

  4. NF-kB activity-dependent P-selectin involved in ox-LDL-induced foam cell formation in U937 cell

    SciTech Connect (OSTI)

    Wang, Yi, E-mail: wangyi2004a@126.com [Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080 (China)] [Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080 (China); Wang, Xiang; Sun, Minghui; Zhang, Zhenyu; Cao, Heng; Chen, Xiaoqing [Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080 (China)] [Department of Cardiology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080 (China)

    2011-08-05T23:59:59.000Z

    Highlights: {yields} Ox-LDL induced foam cell formation in the human U937 promonocytic cell line in a dose- and time-dependent manner. {yields} Ox-LDL induced expression of P-selectin through degradation of IkBa and augment of NF-kB activity and protein level during macrophage-derived foam cell formation. {yields} P-selectin and NF-kB may be identified as pivotal regulators of ox-LDL-induced foam cell formation. {yields} Therapy based on the inhibition of P-selectin and NF-kB may complement conventional treatments to prevent atherosclerosis. -- Abstract: Oxidized low-density lipoprotein (ox-LDL) plays a critical role in regulation of atherosclerosis. However, little is known about the role of Nuclear factor kB (NF-kB) activity-dependent P-selectin in ox-LDL-induced foam cell formation during atherosclerosis. In this study, we first investigated ox-LDL induced foam cell formation in the human U937 promonocytic cell line in a dose- and time-dependent manner. Treatment of U937 cells with ox-LDL increased lipid accumulation as well as intracellular cholesterol content. Next, a comparative analysis of gene expression profiling using cDNA microarray and Real-time-PCR indicated that ox-LDL exposure induced, in three treated groups, an extremely marked increase in the mRNA level of P-selectin. Protein levels of P-selectin and its upstream regulators IkBa and NF-kB showed that NF-kB pathway is involved in the ox-LDL-induced foam cell formation. Finally, overexpression of NF-kB significantly accelerated, whereas, inhibition of NF-kB with siRNA remarkably attenuated ox-LDL-induced macrophage-derived foam cell formation. It was concluded that the activity of NF-kB is augmented during macrophage-derived foam cell formation. Activation of NF-kB increased, whereas, inhibition of NF-kB decreased ox-LDL-induced P-selectin expression and lipid accumulation in macrophages, suggesting ox-LDL induced expression of P-selectin through degradation of IkBa and activation of NF-kB in the regulation of foam cell formation.

  5. Thermal Reactions of Uranium Metal, UO2, U3O8, UF4, and UO2F2 with NF3 to Produce UF6

    SciTech Connect (OSTI)

    McNamara, Bruce K.; Scheele, Randall D.; Kozelisky, Anne E.; Edwards, Matthew K.

    2009-11-01T23:59:59.000Z

    he objective of this paper is to demonstrate that NF3 fluorinates uranium metal, UO2, UF4, UO3, U3O8, and UO2F22H2O to produce the volatile UF6 at temperatures between 100 and 500?C. Thermogravimetric reaction profiles are described that reflect changes in the uranium oxidation state and discrete chemical speciation. Differences in the onset temperatures for each system indicate that NF3-substrate interactions are important for the temperature at which NF3 reacts: U metal > UO3 > UO2 > UO2F2 > UF4 and in fact may indicate different fluorination mechanisms for these various substrates. These studies demonstrate that NF3 is a potential replacement fluorinating agent in the existing nuclear fuel cycle and in oft-proposed actinide volatility reprocessing.

  6. 1 Managed by UT-Battelle for the U.S. Department of Energy NF/IDS Hg Vessel Layout 30 Jun 09

    E-Print Network [OSTI]

    McDonald, Kirk

    1 Managed by UT-Battelle for the U.S. Department of Energy NF/IDS Hg Vessel Layout 30 Jun 09 Cryostat 2 Front Drain Mercury Vessel Concept Matthew F. Glisson Van Graves #12;2 Managed by UT-Battelle;3 Managed by UT-Battelle for the U.S. Department of Energy NF/IDS Hg Vessel Layout 30 Jun 09 Cross Section

  7. Nmero Nome 1T 2T R1T R2T E AC NF 42200 Armando Dias

    E-Print Network [OSTI]

    Baia, Margarida

    Sheet1 Page 1 Nmero Nome 1T 2T R1T R2T E AC NF 42200 Armando Dias 53515 Jorge Branco 55774 Joo 70100 Catarina Ferreira 70146 Mariana Castro 70364 Nuno Gaspar 70524 Antnio Grego 10 2 5 70934 Joo.2 2 15 78167 Afonso Sarmento 13.8 8.3 7.6 2 12 78175 Carlos Martins 14.3 12.6 11 2 14 78183 Ana

  8. Sulforaphane protects against cytokine- and streptozotocin-induced {beta}-cell damage by suppressing the NF-{kappa}B pathway

    SciTech Connect (OSTI)

    Song, Mi-Young; Kim, Eun-Kyung [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk, 561-756 (Korea, Republic of); Moon, Woo-Sung [Department of Pathology, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk, 561-756 (Korea, Republic of); Park, Jin-Woo [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk, 561-756 (Korea, Republic of); Kim, Hyung-Jin; So, Hong-Seob; Park, Raekil [Vestibulocochlear System Research Center, College of Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749 (Korea, Republic of); Kwon, Kang-Beom [Department of Physiology, School of Oriental Medicine, College of Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749 (Korea, Republic of); Vestibulocochlear System Research Center, College of Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749 (Korea, Republic of)], E-mail: desson@wonkwang.ac.kr; Park, Byung-Hyun [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk, 561-756 (Korea, Republic of)], E-mail: bhpark@chonbuk.ac.kr

    2009-02-15T23:59:59.000Z

    Sulforaphane (SFN) is an indirect antioxidant that protects animal tissues from chemical or biological insults by stimulating the expression of several NF-E2-related factor-2 (Nrf2)-regulated phase 2 enzymes. Treatment of RINm5F insulinoma cells with SFN increases Nrf2 nuclear translocation and expression of phase 2 enzymes. In this study, we investigated whether the activation of Nrf2 by SFN treatment or ectopic overexpression of Nrf2 inhibited cytokine-induced {beta}-cell damage. Treatment of RIN cells with IL-1{beta} and IFN-{gamma} induced {beta}-cell damage through a NF-{kappa}B-dependent signaling pathway. Activation of Nrf2 by treatment with SFN and induction of Nrf2 overexpression by transfection with Nrf2 prevented cytokine toxicity. The mechanism by which Nrf2 activation inhibited NF-{kappa}B-dependent cell death signals appeared to involve the reduction of oxidative stress, as demonstrated by the inhibition of cytokine-induced H{sub 2}O{sub 2} production. The protective effect of SFN was further demonstrated by the restoration of normal insulin secreting responses to glucose in cytokine-treated rat pancreatic islets. Furthermore, pretreatment with SFN blocked the development of type 1 diabetes in streptozotocin-treated mice.

  9. Etching of UO{sub 2} in NF{sub 3} RF Plasma Glow Discharge

    SciTech Connect (OSTI)

    John M. Veilleux

    1999-08-01T23:59:59.000Z

    A series of room temperature, low pressure (10.8 to 40 Pa), low power (25 to 210 W) RF plasma glow discharge experiments with UO{sub 2} were conducted to demonstrate that plasma treatment is a viable method for decontaminating UO{sub 2} from stainless steel substrates. Experiments were conducted using NF{sub 3} gas to decontaminate depleted uranium dioxide from stainless-steel substrates. Depleted UO{sub 2} samples each containing 129.4 Bq were prepared from 100 microliter solutions of uranyl nitrate hexahydrate solution. The amorphous UO{sub 2} in the samples had a relatively low density of 4.8 gm/cm{sub 3}. Counting of the depleted UO{sub 2} on the substrate following plasma immersion was performed using liquid scintillation counting with alpha/beta discrimination due to the presence of confounding beta emitting daughter products, {sup 234}Th and {sup 234}Pa. The alpha emission peak from each sample was integrated using a gaussian and first order polynomial fit to improve quantification. The uncertainties in the experimental measurement of the etched material were estimated at about {+-} 2%. Results demonstrated that UO{sub 2} can be completely removed from stainless-steel substrates after several minutes processing at under 200 W. At 180 W and 32.7 Pa gas pressure, over 99% of all UO{sub 2} in the samples was removed in just 17 minutes. The initial etch rate in the experiments ranged from 0.2 to 7.4 {micro}m/min. Etching increased with the plasma absorbed power and feed gas pressure in the range of 10.8 to 40 Pa. A different pressure effect on UO{sub 2} etching was also noted below 50 W in which etching increased up to a maximum pressure, {approximately}23 Pa, then decreased with further increases in pressure.

  10. Renormalization of local quark-bilinear operators for Nf=3 flavors of SLiNC fermions

    E-Print Network [OSTI]

    M. Constantinou; R. Horsley; H. Panagopoulos; H. Perlt; P. E. L. Rakow; G. Schierholz; A. Schiller; J. M. Zanotti

    2014-08-26T23:59:59.000Z

    The renormalization factors of local quark-bilinear operators are computed non-perturbatively for $N_f=3$ flavors of SLiNC fermions, with emphasis on the various procedures for the chiral and continuum extrapolations. The simulations are performed at a lattice spacing $a=0.074$ fm, and for five values of the pion mass in the range of 290-465 MeV, allowing a safe and stable chiral extrapolation. Emphasis is given in the subtraction of the well-known pion pole which affects the renormalization factor of the pseudoscalar current. We also compute the inverse propagator and the Green's functions of the local bilinears to one loop in perturbation theory. We investigate lattice artifacts by computing them perturbatively to second order as well as to all orders in the lattice spacing. The renormalization conditions are defined in the RI$'$-MOM scheme, for both the perturbative and non-perturbative results. The renormalization factors, obtained at different values of the renormalization scale, are translated to the ${\\bar{\\rm MS}}$ scheme and are evolved perturbatively to 2 GeV. Any residual dependence on the initial renormalization scale is eliminated by an extrapolation to the continuum limit. We also study the various sources of systematic errors. Particular care is taken in correcting the non-perturbative estimates by subtracting lattice artifacts computed to one loop perturbation theory using the same action. We test two different methods, by subtracting either the ${\\cal O}(g^2\\,a^2)$ contributions, or the complete (all orders in $a$) one-loop lattice artifacts.

  11. Determination of c_sw in N_f=3+1 Lattice QCD with massive Wilson fermions

    E-Print Network [OSTI]

    Patrick Fritzsch; Rainer Sommer; Felix Stollenwerk; Ulli Wolff

    2015-01-27T23:59:59.000Z

    We develop a strategy for the non-perturbative determination of the O(a)-improvement coefficient c_sw for Wilson fermions with massive sea quarks. The improvement condition is defined via the PCAC relation in the Schr\\"odinger functional. It is imposed along a line of constant physics designed to be close to the correct mass of the charm quark. The numerical work uses the tree-level improved L\\"uscher-Weisz gauge action in N_f=3+1 Lattice QCD.

  12. A (P)HMC algorithm for N_f = 2 + 1 + 1 flavours of twisted mass fermions

    E-Print Network [OSTI]

    T. Chiarappa; R. Frezzotti; C. Urbach

    2005-09-27T23:59:59.000Z

    We present a detailed design of a (P)HMC simulation algorithm for N_f = 2 + 1 + 1 maximally twisted Wilson quark flavours. The algorithm retains even/odd and mass-shift preconditionings combined with multiple Molecular Dynamics time scales for both the light mass degenerate, u and d, quarks and the heavy mass non-degenerate, s and c, quarks. Various non-standard aspects of the algorithm are discussed, among which those connected to the use of a polynomial approximation for the inverse (square root of the squared) Dirac matrix in the s and c quark sector.

  13. Natural Proteasome Inhibitor Celastrol Suppresses Androgen-Independent Prostate Cancer Progression by Modulating Apoptotic Proteins and NF-kappaB

    E-Print Network [OSTI]

    Dai, Yao; Jeffrey, Tang; Wenhua, Xiaojie; Meng, Yang; Burstein, Ezra; Lawrence, Theodore S.; Xu, Liang

    2010-12-10T23:59:59.000Z

    Apoptotic Proteins and NF-kappaB. PLoS ONE 5(12): e14153. doi:10.1371/journal.pone.0014153 Editor: Gen Sheng Wu, Wayne State University, United States of America Received July 10, 2010; Accepted November 10, 2010; Published December 10, 2010 Copyright: #2... through a University of Michigan Cancer Center Support Grant [5 P30 CA46592]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared...

  14. NF-{kappa}B signaling is activated and confers resistance to apoptosis in three-dimensionally cultured EGFR-mutant lung adenocarcinoma cells

    SciTech Connect (OSTI)

    Sakuma, Yuji, E-mail: ysakuma@gancen.asahi.yokohama.jp [Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, Yokohama (Japan)] [Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, Yokohama (Japan); Yamazaki, Yukiko; Nakamura, Yoshiyasu; Yoshihara, Mitsuyo; Matsukuma, Shoichi; Koizume, Shiro; Miyagi, Yohei [Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, Yokohama (Japan)] [Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, Yokohama (Japan)

    2012-07-13T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer EGFR-mutant cells in 3D culture resist EGFR inhibition compared with suspended cells. Black-Right-Pointing-Pointer Degradation of I{kappa}B and activation of NF-{kappa}B are observed in 3D-cultured cells. Black-Right-Pointing-Pointer Inhibiting NF-{kappa}B enhances the efficacy of the EGFR inhibitor in 3D-cultured cells. -- Abstract: Epidermal growth factor receptor (EGFR)-mutant lung adenocarcinoma cells in suspension undergo apoptosis to a greater extent than adherent cells in a monolayer when EGFR autophosphorylation is inhibited by EGFR tyrosine kinase inhibitors (TKIs). This suggests that cell adhesion to a culture dish may activate an anti-apoptotic signaling pathway other than the EGFR pathway. Since the microenvironment of cells cultured in a monolayer are substantially different to that of cells existing in three-dimension (3D) in vivo, we assessed whether two EGFR-mutant lung adenocarcinoma cell lines, HCC827 and H1975, were more resistant to EGFR TKI-induced apoptosis when cultured in a 3D extracellular matrix (ECM) as compared with in suspension. The ECM-adherent EGFR-mutant cells in 3D were significantly less sensitive to treatment with WZ4002, an EGFR TKI, than the suspended cells. Further, a marked degradation of I{kappa}B{alpha}, the inhibitor of nuclear factor (NF)-{kappa}B, was observed only in the 3D-cultured cells, leading to an increase in the activation of NF-{kappa}B. Moreover, the inhibition of NF-{kappa}B with pharmacological inhibitors enhanced EGFR TKI-induced apoptosis in 3D-cultured EGFR-mutant cells. These results suggest that inhibition of NF-{kappa}B signaling would render ECM-adherent EGFR-mutant lung adenocarcinoma cells in vivo more susceptible to EGFR TKI-induced cell death.

  15. Determination of the running coupling constant $?_s$ for Nf=2+1 QCD with the Schroedinger functional scheme

    E-Print Network [OSTI]

    Yusuke Taniguchi; for the PACS-CS collaboration

    2009-10-27T23:59:59.000Z

    We present an evaluation of the running coupling constant and the quark mass renormalization factor for $N_f=2+1$ QCD. The Schr\\"odinger functional scheme is used as the intermediate scheme to carry out non-perturbative running from the low energy region, where physical input is introduced, to deep in the high energy perturbative region, where conversion to the ${\\ovl{\\rm MS}}$ scheme is safely performed. For numerical simulations we adopted Iwasaki gauge action and non-perturbatively improved Wilson fermion action with the clover term. Seven renormalization scales are used to cover from low to high energy region and three lattice spacings to take the continuum limit at each scale. Physical inputs are introduced from the previous $N_f=2+1$ simulation of the CP-PACS/JL-QCD collaboration, which covered the up-down quark mass range heavier than $m_\\pi\\sim 500$ MeV, and that of PACS-CS collaboration for much lighter quark masses down to $m_\\pi=155$ MeV.

  16. Angiotensin II modulates interleukin-1{beta}-induced inflammatory gene expression in vascular smooth muscle cells via interfering with ERK-NF-{kappa}B crosstalk

    SciTech Connect (OSTI)

    Xu, Shanqin [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States)] [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States); Zhi, Hui [Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (United States)] [Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (United States); Hou, Xiuyun [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States)] [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States); Jiang, Bingbing, E-mail: bjiang1@rics.bwh.harvard.edu [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States) [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States); Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (United States)

    2011-07-08T23:59:59.000Z

    Highlights: {yields} We examine how angiotensin II modulates ERK-NF-{kappa}B crosstalk and gene expression. {yields} Angiotensin II suppresses IL-1{beta}-induced prolonged ERK and NF-{kappa}B activation. {yields} ERK-RSK1 signaling is required for IL-1{beta}-induced prolonged NF-{kappa}B activation. {yields} Angiotensin II modulates NF-{kappa}B responsive genes via regulating ERK-NF-{kappa}B crosstalk. {yields} ERK-NF-{kappa}B crosstalk is a novel mechanism regulating inflammatory gene expression. -- Abstract: Angiotensin II is implicated in cardiovascular diseases, which is associated with a role in increasing vascular inflammation. The present study investigated how angiotensin II modulates vascular inflammatory signaling and expression of inducible nitric oxide synthase (iNOS) and vascular cell adhesion molecule (VCAM)-1. In cultured rat aortic vascular smooth muscle cells (VSMCs), angiotensin II suppressed interleukin-1{beta}-induced prolonged phosphorylation of extracellular signal-regulated kinase (ERK) and ribosomal S6 kinase (RSK)-1, and nuclear translocation of nuclear factor (NF)-{kappa}B, leading to decreased iNOS but enhanced VCAM-1 expression, associated with an up-regulation of mitogen-activated protein kinase phosphatase-1 expression. Knock-down of RSK1 selectively down regulated interleukin-1{beta}-induced iNOS expression without influencing VCAM-1 expression. In vivo experiments showed that interleukin-1{beta}, iNOS, and VCAM-1 expression were detectable in the aortic arches of both wild-type and apolipoprotein E-deficient (ApoE{sup -/-}) mice. VCAM-1 and iNOS expression were higher in ApoE{sup -/-} than in wild type mouse aortic arches. Angiotensin II infusion (3.2 mg/kg/day, for 6 days, via subcutaneous osmotic pump) in ApoE{sup -/-} mice enhanced endothelial and adventitial VCAM-1 and iNOS expression, but reduced medial smooth muscle iNOS expression associated with reduced phosphorylation of ERK and RSK-1. These results indicate that angiotensin II can differentially modulate inflammatory gene expression in aortic smooth muscle cells through influencing ERK-NF-{kappa}B crosstalk, which may contribute to angiotensin II-induced inflammatory disorders related to cardiovascular diseases.

  17. Apolipoprotein E inhibits osteoclast differentiation via regulation of c-Fos, NFATc1 and NF-?B

    SciTech Connect (OSTI)

    Kim, Woo-Shin; Kim, Hyung Joon; Lee, Zang Hee [Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749 (Korea, Republic of); Lee, Youngkyun [Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Kim, Hong-Hee, E-mail: hhbkim@snu.ac.kr [Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749 (Korea, Republic of)

    2013-02-15T23:59:59.000Z

    Apolipoprotein E (ApoE) plays a major role in the transport and metabolism of lipid. Other functions of ApoE include modulation of innate and adaptive immune responses. The expression of ApoE in osteoblasts and its relevance with bone formation have also been reported. However, the effect of ApoE on osteoclasts has not yet been examined. Here, we investigated the role of ApoE in osteoclast differentiation using bone marrow-derived macrophages (BMMs) and RAW264.7 cells. We found a down-regulation of ApoE gene expression during osteoclastic differentiation of those cells. Overexpression of ApoE in BMMs and RAW264.7 cells significantly blocked the induction of c-Fos and nuclear factor of activated T cell c1 (NFATc1), transcription factors critical for expression of osteoclast marker genes, by receptor activator of nuclear factor ?B ligand (RANKL), the osteoclast differentiation factor. ApoE inhibited osteoclast differentiation, as measured by decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (MNCs). In addition, ApoE reduced the expression of dendritic cell-specific transmembrane protein (DC-STAMP) and ATPase, H{sup +} transporting, lysosomal 38 kDa, V0 subunit d2 (ATP6v0d2), genes involved in cellcell fusion during osteoclastogenesis. Knock-down of ApoE using a specific siRNA promoted the RANKL-mediated induction of osteoclast differentiation. While ApoE did not affect the activation of ERK, JNK, and p38 MAPK signaling pathways by RANKL, the phosphorylation of p65 trans-activation domain on serine 536 and transcription activity of NF-?B were reduced by ApoE overexpression. These findings suggest that ApoE plays an inhibitory role in osteoclast differentiation via the suppression of RANKL-dependent activation of NF-?B and induction of c-Fos and NFATc1. - Highlights: ? Apolipoprotein E (ApoE) significantly inhibited osteoclast differentiation and activation of NF-?B. ? ApoE decreased the induction of osteoclast marker genes such as c-Fos, NFATc1, TRAP, DC-STAMP and Cathepsin K. ? ApoE reduced number of TRAP-positive multinuclear cells. ? Exogenous ApoE inhibits osteoclast formation by reduction of crucial factor of osteoclast differentiation. ? knock-down of ApoE promoted the RANKL-mediated induction of osteoclast differentiation.

  18. GRP94/gp96 Elicits ERK Activation in Murine Macrophages A ROLE FOR ENDOTOXIN CONTAMINATION IN NF-B ACTIVATION AND NITRIC OXIDE PRODUCTION*

    E-Print Network [OSTI]

    Nicchitta, Chris

    GRP94/gp96 Elicits ERK Activation in Murine Macrophages A ROLE FOR ENDOTOXIN CONTAMINATION IN NF- B signaling and ni- tric oxide production, as well as the MAP kinase p38, JNK, and ERK signaling cascades a marked increase in ERK phosphorylation at protein concentrations as low as 2 g/ml. These results

  19. Differential Effects of ?-catenin and NF-?B Interplay in the Regulation of Cell Proliferation, Inflammation and Tumorigenesis in Response to Bacterial Infection

    E-Print Network [OSTI]

    Chandrakesan, Parthasarathy; Jakkula, Laxmi Uma Maheswar Rao; Ahmed, Ishfaq; Roy, Badal; Anant, Shrikant; Umar, Shahid

    2013-11-21T23:59:59.000Z

    Both ?-catenin and NF-?B have been implicated in our laboratory as candidate factors in driving proliferation in an in vivo model of Citrobacter rodentium (CR)-induced colonic crypt hyper-proliferation and hyperplasia. Herein, we test the hypothesis...

  20. Spontaneous transition of core radial electric field driven by magnetic islands in the H-1NF heliac

    SciTech Connect (OSTI)

    Kumar, S. T. A. [University of Wisconsin, Madison; Blackwell, B. D. [Australian National University, Canberra, Australia; Howard, J. [Australian National University, Canberra, Australia; Harris, J. H. [Oak Ridge National Laboratory (ORNL)

    2011-01-01T23:59:59.000Z

    This paper reports an experimental observation of spontaneous transition of the core radial electric field to a large positive value (E(r) similar to 5 kV m(-1)), with a strong electric-field shear (similar to 700 kV m(-2)) in a low temperature (T(e) similar to 10 eV) radio frequency generated argon plasma in the H-1NF heliac stellarator. The transition, which seems to be driven by a spontaneous excitation of m = 2 magnetic islands near the core, is associated with a localized increase in the plasma density and excitation of coherent low frequency (similar to 3 kHz) oscillations possibly due to unstable E(r) shear driven modes. Evidence suggests development of the core electron-root scenario, which previously has been observed only at high temperature electron cyclotron heated plasmas.

  1. Absolute frequency measurements of 85Rb nF7/2 Rydberg states using purely optical detection

    E-Print Network [OSTI]

    L. A. M. Johnson; H. O. Majeed; B. Sanguinetti; Th. Becker; B. T. H. Varcoe

    2010-02-16T23:59:59.000Z

    A three-step laser excitation scheme is used to make absolute frequency measurements of highly excited nF7/2 Rydberg states in 85Rb for principal quantum numbers n=33-100. This work demonstrates the first absolute frequency measurements of rubidium Rydberg levels using a purely optical detection scheme. The Rydberg states are excited in a heated Rb vapour cell and Doppler free signals are detected via purely optical means. All of the frequency measurements are made using a wavemeter which is calibrated against a GPS disciplined self-referenced optical frequency comb. We find that the measured levels have a very high frequency stability, and are especially robust to electric fields. The apparatus has allowed measurements of the states to an accuracy of 8.0MHz. The new measurements are analysed by extracting the modified Rydberg-Ritz series parameters.

  2. Magnetic susceptibility and equation of state of N_f = 2+1 QCD with physical quark masses

    E-Print Network [OSTI]

    Claudio Bonati; Massimo D'Elia; Marco Mariti; Francesco Negro; Francesco Sanfilippo

    2014-05-13T23:59:59.000Z

    We determine the free energy of strongly interacting matter as a function of an applied constant and uniform magnetic field. We consider N_f = 2+1 QCD with physical quark masses, discretized on a lattice by stout improved staggered fermions and a tree level improved Symanzik pure gauge action, and explore three different lattice spacings. For magnetic fields of the order of those produced in non-central heavy ion collisions (eB ~ 0.1 GeV^2) strongly interacting matter behaves like a medium with a linear response, and is paramagnetic both above and below the deconfinement transition, with a susceptibility which steeply rises in the deconfined phase. We compute the equation of state, showing that the relative increase in the pressure due to the magnetic field gets larger around the transition, and of the order of 10 % for eB ~ 0.1 GeV^2.

  3. Metastatic function of BMP-2 in gastric cancer cells: The role of PI3K/AKT, MAPK, the NF-{kappa}B pathway, and MMP-9 expression

    SciTech Connect (OSTI)

    Kang, Myoung Hee [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Oh, Sang Cheul [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Lee, Hyun Joo [Department of Pathology, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Department of Pathology, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Kang, Han Na; Kim, Jung Lim [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Kim, Jun Suk [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Yoo, Young A., E-mail: ydanbi@korea.ac.kr [Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)

    2011-07-15T23:59:59.000Z

    Bone morphogenetic proteins (BMPs) have been implicated in tumorigenesis and metastatic progression in various types of cancer cells, but the role and cellular mechanism in the invasive phenotype of gastric cancer cells is not known. Herein, we determined the roles of phosphoinositide 3-kinase (PI3K)/AKT, extracellular signal-regulated protein kinase (ERK), nuclear factor (NF)-{kappa}B, and matrix metalloproteinase (MMP) expression in BMP-2-mediated metastatic function in gastric cancer. We found that stimulation of BMP-2 in gastric cancer cells enhanced the phosphorylation of AKT and ERK. Accompanying activation of AKT and ERK kinase, BMP-2 also enhanced phosphorylation/degradation of I{kappa}B{alpha} and the nuclear translocation/activation of NF-{kappa}B. Interestingly, blockade of PI3K/AKT and ERK signaling using LY294002 and PD98059, respectively, significantly inhibited BMP-2-induced motility and invasiveness in association with the activation of NF-{kappa}B. Furthermore, BMP-2-induced MMP-9 expression and enzymatic activity was also significantly blocked by treatment with PI3K/AKT, ERK, or NF-{kappa}B inhibitors. Immunohistochemistry staining of 178 gastric tumor biopsies indicated that expression of BMP-2 and MMP-9 had a significant positive correlation with lymph node metastasis and a poor prognosis. These results indicate that the BMP-2 signaling pathway enhances tumor metastasis in gastric cancer by sequential activation of the PI3K/AKT or MAPK pathway followed by the induction of NF-{kappa}B and MMP-9 activity, indicating that BMP-2 has the potential to be a therapeutic molecular target to decrease metastasis.

  4. Inhibition of inflammatory mediators contributes to the anti-inflammatory activity of KYKZL-1 via MAPK and NF-?B pathway

    SciTech Connect (OSTI)

    Xu, Guang-Lin [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Department of Pharmacology, University of Michigan, Ann Arbor (United States); Du, Yi-Fang; Cheng, Jing; Huan, Lin [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Chen, Shi-Cui [Jinhu Food and Drug Administration, Jiangsu (China); Wei, Shao-Hua [College of Chemistry and Materials Science, Nanjing Normal University, Nanjing (China); Gong, Zhu-Nan, E-mail: biopharmacology@126.com [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Cai, Jie; Qiu, Ting; Wu, Hao; Sun, Ting [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Ao, Gui-Zhen [Department of Medicinal Chemistry, School of Pharmacy, Soochow University, Jiangsu (China)

    2013-10-01T23:59:59.000Z

    KYKZL-1, a newly synthesized compound with COX/5-LOX dual inhibition, was subjected to the anti-inflammatory activity test focusing on its modulation of inflammatory mediators as well as intracellular MAPK and NF-?B signaling pathways. In acute ear edema model, pretreatment with KYKZL-1 (p.o.) dose-dependently inhibited the xylene-induced ear edema in mice with a higher inhibition than diclofenac. In a three-day TPA-induced inflammation, KYKZL-1 also showed significant anti-inflammatory activity with inhibition ranging between 20% and 64%. In gastric lesion test, KYKZL-1 elicited markedly fewer stomach lesions with a low index of ulcer as compared to diclofenac in rats. In further studies, KYKZL-1 was found to significantly inhibit the production of NO, PGE{sub 2}, LTB{sub 4} in LPS challenged RAW264.7, which is parallel to its attenuation of the expression of iNOS, COX-2, 5-LOX mRNAs or proteins and inhibition of phosphorylation of p38 and ERK MAPKs and activation of NF-?B. Taken together, our data indicate that KYKZL-1 comprises dual inhibition of COX and 5-LOX and exerts an obvious anti-inflammatory activity with an enhanced gastric safety profile via simultaneous inhibition of phosphorylation of p38 and ERK MAPKs and activation of NF-?B. - Highlights: KYKZL-1 is designed to exhibit COX/5-LOX dual inhibition. KYKZL-1 inhibits NO, PGE{sub 2} and LTB{sub 4} and iNOS, COX-2 and 5-LOX mRNAs and MAPKs. KYKZL-1 inhibits phosphorylation of MAPKs. KYKZL-1 inactivates NF-?B pathway.

  5. Carbachol ameliorates lipopolysaccharide-induced intestinal epithelial tight junction damage by down-regulating NF-{kappa}{beta} and myosin light-chain kinase pathways

    SciTech Connect (OSTI)

    Zhang, Ying [Department of Anesthesia, Critical Care Medicine and Emergency Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, People's Republic of China (China)] [Department of Anesthesia, Critical Care Medicine and Emergency Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, People's Republic of China (China); Li, Jianguo, E-mail: 2010lijianguo@sina.cn [Department of Anesthesia, Critical Care Medicine and Emergency Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, People's Republic of China (China)] [Department of Anesthesia, Critical Care Medicine and Emergency Medicine Center, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, People's Republic of China (China)

    2012-11-16T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Carbachol reduced the lipopolysaccharide-induced intestinal barrier breakdown. Black-Right-Pointing-Pointer Carbachol ameliorated the lipopolysaccharide-induced ileal tight junction damage. Black-Right-Pointing-Pointer Carbachol prevented the LPS-induced NF-{kappa}{beta} and myosin light-chain kinase activation. Black-Right-Pointing-Pointer Carbachol exerted its beneficial effects in an {alpha}7 nicotinic receptor-dependent manner. -- Abstract: Carbachol is a cholinergic agonist that protects the intestines after trauma or burn injury. The present study determines the beneficial effects of carbachol and the mechanisms by which it ameliorates the lipopolysaccharide (LPS)-induced intestinal barrier breakdown. Rats were injected intraperitoneally with 10 mg/kg LPS. Results showed that the gut barrier permeability was reduced, the ultrastructural disruption of tight junctions (TJs) was prevented, the redistribution of zonula occludens-1 and claudin-2 proteins was partially reversed, and the nuclear factor-kappa beta (NF-{kappa}{beta}) and myosin light-chain kinase (MLCK) activation in the intestinal epithelium were suppressed after carbachol administration in LPS-exposed rats. Pretreatment with the {alpha}7 nicotinic acetylcholine receptor ({alpha}7nAchR) antagonist {alpha}-bungarotoxin blocked the protective action of carbachol. These results suggested that carbachol treatment can protect LPS-induced intestinal barrier dysfunction. Carbachol exerts its beneficial effect on the amelioration of the TJ damage by inhibiting the NF-{kappa}{beta} and MLCK pathways in an {alpha}7nAchR-dependent manner.

  6. Broken Valence Chiral Symmetry and Chiral Polarization of Dirac Spectrum in N$_f$=12 QCD at Small Quark Mass

    E-Print Network [OSTI]

    Andrei Alexandru; Ivan Horvth

    2014-12-04T23:59:59.000Z

    The validity of recently proposed equivalence between valence spontaneous chiral symmetry breaking (vSChSB) and chiral polarization of low energy Dirac spectrum (ChP) in SU(3) gauge theory, is examined for the case of twelve mass-degenerate fundamental quark flavors. We find that the vSChSB-ChP correspondence holds for regularized systems studied. Moreover, our results suggest that vSChSB occurs in two qualitatively different circumstances: there is a quark mass $m_c$ such that for $m > m_c$ the mode condensing Dirac spectrum exhibits standard monotonically increasing density, while for $m_{ch} < m < m_c$ the peak around zero separates from the bulk of the spectrum, with density showing a pronounced depletion at intermediate scales. Valence chiral symmetry restoration may occur at yet smaller masses $m < m_{ch}$, but this has not yet been seen by overlap valence probe, leaving the $m_{ch}=0$ possibility open. The latter option could place massless N$_f$=12 theory outside of conformal window. Anomalous behavior of overlap Dirac spectrum for $m_{ch} < m < m_c$ is qualitatively similar to one observed previously in zero and few-flavor theories as an effect of thermal agitation.

  7. Up, down, strange and charm quark masses with Nf = 2+1+1 twisted mass lattice QCD

    E-Print Network [OSTI]

    N. Carrasco; A. Deuzeman; P. Dimopoulos; R. Frezzotti; V. Gimenez; G. Herdoiza; P. Lami; V. Lubicz; D. Palao; E. Picca; S. Recker; L. Riggio; G. C. Rossi; F. Sanfilippo; L. Scorzato; S. Simula; C. Tarantino; C. Urbach; U. Wenger

    2014-08-21T23:59:59.000Z

    We present a lattice QCD calculation of the up, down, strange and charm quark masses performed using the gauge configurations produced by the European Twisted Mass Collaboration with Nf = 2 + 1 + 1 dynamical quarks, which include in the sea, besides two light mass degenerate quarks, also the strange and charm quarks with masses close to their physical values. The simulations are based on a unitary setup for the two light quarks and on a mixed action approach for the strange and charm quarks. The analysis uses data at three values of the lattice spacing and pion masses in the range 210 - 450 MeV, allowing for accurate continuum limit and controlled chiral extrapolation. The quark mass renormalization is carried out non-perturbatively using the RI-MOM method. The results for the quark masses converted to the bar{MS} scheme are: mud(2 GeV) = 3.70(17) MeV, ms(2 GeV) = 99.6(4.3) MeV and mc(mc) = 1.348(46) GeV. We obtain also the quark mass ratios ms/mud = 26.66(32) and mc/ms = 11.62(16). By studying the mass splitting between the neutral and charged kaons and using available lattice results for the electromagnetic contributions, we evaluate mu/md = 0.470(56), leading to mu = 2.36(24) MeV and md = 5.03(26) MeV.

  8. Rosiglitazone attenuates NF-{kappa}B-dependent ICAM-1 and TNF-{alpha} production caused by homocysteine via inhibiting ERK{sub 1/2}/p38MAPK activation

    SciTech Connect (OSTI)

    Bai, Yong-Ping [Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China); Liu, Yu-Hui [Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006 (China); Chen, Jia [Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China); Song, Tao [Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410078 (China); You, Yu [Department of Digestive Medicine, Xiangya Second Hospital, Central South University, Changsha, Hunan 410078 (China); Tang, Zhen-Yan [Department of Urologic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China); Li, Yuan-Jian [Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410078 (China); Zhang, Guo-Gang [Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China)]. E-mail: xyzgg2006@sina.com

    2007-08-17T23:59:59.000Z

    Previous studies demonstrated an important interaction between nuclear factor-kappaB (NF-{kappa}B) activation and homocysteine (Hcy)-induced cytokines expression in endothelial cells and vascular smooth muscle cells. However, the underlying mechanism remains illusive. In this study, we investigated the effects of Hcy on NF-{kappa}B-mediated sICAM-1, TNF-{alpha} production and the possible involvement of ERK{sub 1/2}/p38MAPK pathway. The effects of rosiglitazone intervention were also examined. Our results show that Hcy increased the levels of sICAM-1 and TNF-{alpha} in cultured human umbilical vein endothelial cells (HUVECs) in a time- and concentration-dependent manner. This effect was significantly depressed by rosiglitazone and different inhibitors (PDTC, NF-{kappa}B inhibitor; PD98059, MEK inhibitor; SB203580, p38MAPK specific inhibitor; and staurosporine, PKC inhibitor). Next, we investigated the effect of Hcy on ERK{sub 1/2}/p38MAPK pathway and NF-{kappa}B activity in HUVECs. The results show that Hcy activated both ERK{sub 1/2}/p38MAPK pathway and NF-{kappa}B-DNA-binding activity. These effects were markedly inhibited by rosiglitazone as well as other inhibitors (SB203580, PD98059, and PDTC). Further, the pretreatment of staurosporine abrogated ERK{sub 1/2}/p38MAPK phosphorylation, suggesting that Hcy-induced ERK{sub 1/2}/p38MAPK activation is associated with PKC activity. Our results provide evidence that Hcy-induced NF-{kappa}B activation was mediated by activation of ERK{sub 1/2}/p38MAPK pathway involving PKC activity. Rosiglitazone reduces the NF-{kappa}B-mediated sICAM-1 and TNF-{alpha} production induced by Hcy via inhibition of ERK{sub 1/2}/p38MAPK pa0011thw.

  9. Anti-neuroinflammatory efficacy of the aldose reductase inhibitor FMHM via phospholipase C/protein kinase C-dependent NF-?B and MAPK pathways

    SciTech Connect (OSTI)

    Zeng, Ke-Wu [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Li, Jun [Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029 (China); Dong, Xin; Wang, Ying-Hong; Ma, Zhi-Zhong; Jiang, Yong; Jin, Hong-Wei [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Tu, Peng-Fei, E-mail: pengfeitu@vip.163.com [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029 (China)

    2013-11-15T23:59:59.000Z

    Aldose reductase (AR) has a key role in several inflammatory diseases: diabetes, cancer and cardiovascular diseases. Therefore, AR inhibition seems to be a useful strategy for anti-inflammation therapy. In the central nervous system (CNS), microglial over-activation is considered to be a central event in neuroinflammation. However, the effects of AR inhibition in CNS inflammation and its underlying mechanism of action remain unknown. In the present study, we found that FMHM (a naturally derived AR inhibitor from the roots of Polygala tricornis Gagnep.) showed potent anti-neuroinflammatory effects in vivo and in vitro by inhibiting microglial activation and expression of inflammatory mediators. Mechanistic studies showed that FMHM suppressed the activity of AR-dependent phospholipase C/protein kinase C signaling, which further resulted in downstream inactivation of the I?B kinase/I?B/nuclear factor-kappa B (NF-?B) inflammatory pathway. Therefore, AR inhibition-dependent NF-?B inactivation negatively regulated the transcription and expression of various inflammatory genes. AR inhibition by FMHM exerted neuroprotective effects in lipopolysaccharide-induced neuronmicroglia co-cultures. These findings suggested that AR is a potential target for neuroinflammation inhibition and that FMHM could be an effective agent for treating or preventing neuroinflammatory diseases. - Highlights: FMHM is a natural-derived aldose reductase (AR) inhibitor. FMHM inhibits various neuroinflammatory mediator productions in vitro and in vivo. FMHM inhibits neuroinflammation via aldose reductase/PLC/PKC-dependent NF-?B pathway. FMHM inhibits neuroinflammation via aldose reductase/PLC/PKC-dependent MAPK pathway. FMHM protects neurons against inflammatory injury in microglia-neuron co-cultures.

  10. Impaired NFAT and NF?B activation are involved in suppression of CD40 ligand expression by ?{sup 9}-tetrahydrocannabinol in human CD4{sup +} T cells

    SciTech Connect (OSTI)

    Ngaotepprutaram, Thitirat [Department of Pharmacology and Toxicology, Michigan State University (United States); Center for Integrative Toxicology, Michigan State University (United States); Kaplan, Barbara L.F. [Department of Pharmacology and Toxicology, Michigan State University (United States); Center for Integrative Toxicology, Michigan State University (United States); Neuroscience Program, Michigan State University (United States); Kaminski, Norbert E., E-mail: kamins11@msu.edu [Department of Pharmacology and Toxicology, Michigan State University (United States); Center for Integrative Toxicology, Michigan State University (United States)

    2013-11-15T23:59:59.000Z

    We have previously reported that ?{sup 9}-tetrahydrocannabinol (?{sup 9}-THC), the main psychoactive cannabinoid in marijuana, suppresses CD40 ligand (CD40L) expression by activated mouse CD4{sup +} T cells. CD40L is involved in pathogenesis of many autoimmune and inflammatory diseases. In the present study, we investigated the molecular mechanism of ?{sup 9}-THC-mediated suppression of CD40L expression using peripheral blood human T cells. Pretreatment with ?{sup 9}-THC attenuated CD40L expression in human CD4{sup +} T cells activated by anti-CD3/CD28 at both the protein and mRNA level, as determined by flow cytometry and quantitative real-time PCR, respectively. Electrophoretic mobility shift assays revealed that ?{sup 9}-THC suppressed the DNA-binding activity of both NFAT and NF?B to their respective response elements within the CD40L promoter. An assessment of the effect of ?{sup 9}-THC on proximal T cell-receptor (TCR) signaling induced by anti-CD3/CD28 showed significant impairment in the rise of intracellular calcium, but no significant effect on the phosphorylation of ZAP70, PLC?1/2, Akt, and GSK3?. Collectively, these findings identify perturbation of the calcium-NFAT and NF?B signaling cascade as a key mechanistic event by which ?{sup 9}-THC suppresses human T cell function. - Highlights: ?{sup 9}-THC attenuated CD40L expression in activated human CD4+ T cells. ?{sup 9}-THC suppressed DNA-binding activity of NFAT and NF?B. ?{sup 9}-THC impaired elevation of intracellular Ca2+. ?{sup 9}-THC did not affect phosphorylation of ZAP70, PLC?1/2, Akt, and GSK3?.

  11. The NSVZ beta-function and the Schwinger-Dyson equations for N=1 SQED with N_f flavors, regularized by higher derivatives

    E-Print Network [OSTI]

    K. V. Stepanyantz

    2014-06-08T23:59:59.000Z

    The effective diagram technique based on the Schwinger-Dyson equations is constructed for N=1 SQED with N_f flavors, regularized by higher derivatives. Using these effective diagrams, it is possible to derive the exact NSVZ relation between the beta-function and the anomalous dimension of the matter superfields exactly in all loops, if the renormalization group functions are defined in terms of the bare coupling constant. In particular, we verify that all integrals which give the beta-function defined in terms of the bare coupling constant are integrals of double total derivatives and prove some identities relating Green functions.

  12. OSU-A9 inhibits angiogenesis in human umbilical vein endothelial cells via disrupting AktNF-?B and MAPK signaling pathways

    SciTech Connect (OSTI)

    Omar, Hany A. [Division of Medicinal Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH 43210 (United States); Department of Pharmacology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514 (Egypt); Arafa, El-Shaimaa A. [Department of Pharmacology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514 (Egypt); Salama, Samir A. [Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Cairo 11511 (Egypt); Arab, Hany H. [Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo 11562 (Egypt); Wu, Chieh-Hsi, E-mail: chhswu@mail.cmu.edu.tw [School of Pharmacy, China Medical University, Taichung 40402, Taiwan (China); Weng, Jing-Ru, E-mail: columnster@gmail.com [Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan (China)

    2013-11-01T23:59:59.000Z

    Since the introduction of angiogenesis as a useful target for cancer therapy, few agents have been approved for clinical use due to the rapid development of resistance. This problem can be minimized by simultaneous targeting of multiple angiogenesis signaling pathways, a potential strategy in cancer management known as polypharmacology. The current study aimed at exploring the anti-angiogenic activity of OSU-A9, an indole-3-carbinol-derived pleotropic agent that targets mainly Aktnuclear factor-kappa B (NF-?B) signaling which regulates many key players of angiogenesis such as vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Human umbilical vein endothelial cells (HUVECs) were used to study the in vitro anti-angiogenic effect of OSU-A9 on several key steps of angiogenesis. Results showed that OSU-A9 effectively inhibited cell proliferation and induced apoptosis and cell cycle arrest in HUVECs. Besides, OSU-A9 inhibited angiogenesis as evidenced by abrogation of migration/invasion and Matrigel tube formation in HUVECs and attenuation of the in vivo neovascularization in the chicken chorioallantoic membrane assay. Mechanistically, Western blot, RT-PCR and ELISA analyses showed the ability of OSU-A9 to inhibit MMP-2 production and VEGF expression induced by hypoxia or phorbol-12-myristyl-13-acetate. Furthermore, dual inhibition of AktNF-?B and mitogen-activated protein kinase (MAPK) signaling, the key regulators of angiogenesis, was observed. Together, the current study highlights evidences for the promising anti-angiogenic activity of OSU-A9, at least in part through the inhibition of AktNF-?B and MAPK signaling and their consequent inhibition of VEGF and MMP-2. These findings support OSU-A9's clinical promise as a component of anticancer therapy. - Highlights: The antiangiogenic activity of OSU-A9 in HUVECs was explored. OSU-A9 inhibited HUVECs proliferation, migration, invasion and tube formation. OSU-A9 targeted signaling pathways mediated by Akt-NF-kB, VEGF, and MMP-2. The anti-angiogenic activity of OSU-A9 supports its clinical promise.

  13. NF_Eder_2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Saleshttp://www.fnal.gov/directorate/nalcal/nalcal02_07_05_files/nalcal.gif Directorate1, Issue 23 NETL NEVIS- 97NEWS RELEASE For3

  14. JANEX-1, a JAK3 inhibitor, protects pancreatic islets from cytokine toxicity through downregulation of NF-{kappa}B activation and the JAK/STAT pathway

    SciTech Connect (OSTI)

    Lv, Na; Kim, Eun-Kyung; Song, Mi-Young [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of); Choi, Ha-Na; Moon, Woo Sung [Department of Pathology, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Pathology, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of); Park, Sung-Joo [Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749 (Korea, Republic of)] [Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749 (Korea, Republic of); Park, Jin-Woo [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of); Kwon, Kang-Beom, E-mail: desson@wonkwang.ac.kr [Department of Physiology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749 (Korea, Republic of)] [Department of Physiology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749 (Korea, Republic of); Park, Byung-Hyun, E-mail: bhpark@chonbuk.ac.kr [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Biochemistry, Medical School and Diabetes Research Center, Chonbuk National University, Jeonju, Jeonbuk 561-756 (Korea, Republic of)

    2009-07-15T23:59:59.000Z

    JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic {beta}-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1{beta} and interferon (IFN)-{gamma}-induced {beta}-cell damage using isolated islets. JANEX-1-pretreated islets showed resistance to cytokine toxicity, namely suppressed nitric oxide (NO) production, reduced inducible form of NO synthase (iNOS) expression, and decreased islet destruction. The molecular mechanism by which JANEX-1 inhibits iNOS expression was mediated through suppression of the nuclear factor {kappa}B (NF-{kappa}B) and JAK/signal transducer and activator of transcription (STAT) pathways. Islets treated with the cytokines downregulated the protein levels of suppressor of cytokine signaling (SOCS)-1 and SOCS-3, but pretreatment with JANEX-1 attenuated these decreases. Additionally, islets from JAK3{sup -/-} mice were more resistant to cytokine toxicity than islets from control mice. These results demonstrate that JANEX-1 protects {beta}-cells from cytokine toxicity through suppression of the NF-{kappa}B and JAK/STAT pathways and upregulation of SOCS proteins, suggesting that JANEX-1 may be used to preserve functional {beta}-cell mass.

  15. Early induction of cytokines/cytokine receptors and Cox2, and activation of NF-?B in 4-nitroquinoline 1-oxide-induced murine oral cancer model

    SciTech Connect (OSTI)

    Liu, Yu-Ching [Department of Medical Research, China Medical University Hospital, Taichung 404, Taiwan (China) [Department of Medical Research, China Medical University Hospital, Taichung 404, Taiwan (China); Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan (China); Ho, Heng-Chien; Lee, Miau-Rong [Department of Biochemistry, China Medical University, Taichung 404, Taiwan (China)] [Department of Biochemistry, China Medical University, Taichung 404, Taiwan (China); Lai, Kuang-Chi [Department of Surgery, China Medical University Beigang Hospital, Yunlin 651, Taiwan (China) [Department of Surgery, China Medical University Beigang Hospital, Yunlin 651, Taiwan (China); School of Medicine, China Medical University, Taichung 404, Taiwan (China); Yeh, Chung-Min; Lin, Yueh-Min [Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan (China)] [Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan (China); Ho, Tin-Yun [School of Chinese Medicine, China Medical University, Taichung 404, Taiwan (China)] [School of Chinese Medicine, China Medical University, Taichung 404, Taiwan (China); Hsiang, Chien-Yun, E-mail: cyhsiang@mail.cmu.edu.tw [Department of Microbiology, China Medical University, Taichung 404, Taiwan (China)] [Department of Microbiology, China Medical University, Taichung 404, Taiwan (China); Chung, Jing-Gung, E-mail: jgchung@mail.cmu.edu.tw [Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan (China) [Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan (China); Department of Biotechnology, Asia University, Taichung 413, Taiwan (China)

    2012-07-15T23:59:59.000Z

    The purpose of this study was to identify the genes induced early in murine oral carcinogenesis. Murine tongue tumors induced by the carcinogen, 4-nitroquinoline 1-oxide (4-NQO), and paired non-tumor tissues were subjected to microarray analysis. Hierarchical clustering of upregulated genes in the tumor tissues revealed an association of induced genes with inflammation. Cytokines/cytokine receptors induced early were subsequently identified, clearly indicating their involvement in oral carcinogenesis. Hierarchical clustering also showed that cytokine-mediated inflammation was possibly linked with Mapk6. Cox2 exhibited the greatest extent (918 fold) of induction in the microarray data, and its early induction was observed in a 2 h painting experiment by RT-PCR. MetaCore analysis showed that overexpressed Cox2 may interact with p53 and transcriptionally inhibit expression of several downstream genes. A painting experiment in transgenic mice also demonstrated that NF-?B activates early independently of Cox2 induction. MetaCore analysis revealed the most striking metabolic alterations in tumor tissues, especially in lipid metabolism resulting from the reduction of Ppar? and Rxrg. Reduced expression of Mapk12 was noted, and MetaCore analysis established its relationship with decreased efficiency of Ppar? phosphorylation. In conclusion, in addition to cytokines/cytokine receptors, the early induction of Cox2 and NF-?B activation is involved in murine oral carcinogenesis.

  16. High-loop perturbative renormalization constants for Lattice QCD (III): three-loop quark currents for Iwasaki gauge action and n_f=4 Wilson fermions

    E-Print Network [OSTI]

    M. Brambilla; F. Di Renzo; M. Hasegawa

    2014-06-23T23:59:59.000Z

    This is the third of a series of papers on three-loop computation of renormalization constants for Lattice QCD. Our main point of interest are results for the regularization defined by Iwasaki gauge action and n_f=4 Wilson fermions. Our results for quark bilinears renormalized according to the RI'-MOM scheme can be compared to non-perturbative results. The latter are available for Twisted Mass QCD: being defined in the chiral limit, renormalization constants must be the same. We also address more general problems. In particular, we discuss a few methodological issues connected to summing the perturbative series such as the effectiveness of Boosted Perturbation Theory and the disentanglement of irrelevant and finite volume contributions. Discussing these issues we consider ont only the new results of this paper, but also those for the regularization defined by tree-level Symanzik improved gauge action and n_f=2 Wilson fermions, which we presented in a recent paper of ours. We finally comment to which extent the techniques we put at work in the NSPT context can provide a fresher look into the lattice version of the RI'-MOM scheme.

  17. A novel shogaol analog suppresses cancer cell invasion and inflammation, and displays cytoprotective effects through modulation of NF-?B and Nrf2-Keap1 signaling pathways

    SciTech Connect (OSTI)

    Gan, Fei-Fei; Ling, Hui; Ang, Xiaohui; Reddy, Shridhivya A.; Lee, Stephanie S-H.; Yang, Hong; Tan, Sock-Hoon [Department of Pharmacy, Faculty of Science, National University of Singapore (Singapore); Hayes, John D. [Jacqui Wood Cancer Centre, Division of Cancer Research, Ninewells Hospital and Medical School, University of Dundee, Dundee, Scotland (United Kingdom); Chui, Wai-Keung [Department of Pharmacy, Faculty of Science, National University of Singapore (Singapore); Chew, Eng-Hui, E-mail: phaceh@nus.edu.sg [Department of Pharmacy, Faculty of Science, National University of Singapore (Singapore)

    2013-11-01T23:59:59.000Z

    Natural compounds containing vanilloid and Michael acceptor moieties appear to possess anti-cancer and chemopreventive properties. The ginger constituent shogaol represents one such compound. In this study, the anti-cancer potential of a synthetic novel shogaol analog 3-phenyl-3-shogaol (3-Ph-3-SG) was assessed by evaluating its effects on signaling pathways. At non-toxic concentrations, 3-Ph-3-SG suppressed cancer cell invasion in MDA-MB-231 and MCF-7 breast carcinoma cells through inhibition of PMA-activated MMP-9 expression. At similar concentrations, 3-Ph-3-SG reduced expression of the inflammatory mediators nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and prostanglandin-E{sub 2} (PGE{sub 2}) in RAW 264.7 macrophage-like cells. Inhibition of cancer cell invasion and inflammation by 3-Ph-3-SG were mediated through suppression of the nuclear factor-kappaB (NF-?B) signaling pathway. The 3-Ph-3-SG also demonstrated cytoprotective effects by inducing the antioxidant response element (ARE)-driven genes NAD(P)H quinone oxidoreductase-1 (NQO1) and heme oxygenase-1 (HO-1). Cytoprotection by 3-Ph-3-SG was achieved at least partly through modification of cysteine residues in the E3 ubiquitin ligase substrate adaptor Kelch-like ECH-associated protein 1 (Keap1), which resulted in accumulation of transcription factor NF-E2 p45-related factor 2 (Nrf2). The activities of 3-Ph-3-SG were comparable to those of 6-shogaol, the most abundant naturally-occurring shogaol, and stronger than those of 4-hydroxyl-null deshydroxy-3-phenyl-3-shogaol, which attested the importance of the 4-hydroxy substituent in the vanilloid moiety for bioactivity. In summary, 3-Ph-3-SG is shown to possess activities that modulate stress-associated pathways relevant to multiple steps in carcinogenesis. Therefore, it warrants further investigation of this compound as a promising candidate for use in chemotherapeutic and chemopreventive strategies. - Highlights: Novel shogaol 3-phenyl-3-shogaol suppressed cancer cell invasion and inflammation. Anti-invasive and anti-inflammatory effects were NF-?B-dependent. 3-Phenyl-3-shogaol induced ARE-driven genes to achieve cytoprotection. Cytoprotective effects were brought about by modification of cysteines in Keap1. Chemopreventive activities of 3-phenyl-3-shogaol and 6-shogaol were comparable.

  18. Microtrenching-free two-step reactive ion etching of 4H-SiC using NF{sub 3}/HBr/O{sub 2} and Cl{sub 2}/O{sub 2}

    SciTech Connect (OSTI)

    Tseng, Yuan-Hung, E-mail: yhtseng.ee99g@nctu.edu.tw; Tsui, Bing-Yue [Department of Electronics Engineering, National Chiao Tung University, No. 1001, Daxue Rd., East Dist., Hsinchu City 30010, Taiwan (China)

    2014-05-15T23:59:59.000Z

    In this paper, the authors performed a reactive ion etch of a 4H-SiC substrate with a gas mixture of NF{sub 3}, HBr, and O{sub 2}, resulting in a microtrenching-free etch. The etch rate was 107.8?nm/min, and the selectivity over the oxide hard mask was ?3.85. Cross-sectional scanning electron microscopy showed no microtrenching compared with etches using plasmas of NF{sub 3}, NF{sub 3}/HBr, and NF{sub 3}/O{sub 2}. Analyzing a variety of HBr/O{sub 2} mixing ratios, the authors discuss the additive effect of each gas and their respective potential mechanisms for alleviating microtrenching. To increase the radius of gyration of the bottom corners, they introduced a second etch step with Cl{sub 2}/O{sub 2} plasma. Fabricating simple metal-oxide-semiconductor capacitors on the two-step etched surface, the authors found that the electrical characteristics of the etched sample were nearly the same as the nonetched sample.

  19. Relation between two-point Green functions of ${\\cal N}=1$ SQED with $N_f$ flavors, regularized by higher derivatives, in the three-loop approximation

    E-Print Network [OSTI]

    A. E. Kazantsev; K. V. Stepanyantz

    2014-10-05T23:59:59.000Z

    We verify the identity which relates the two-point Green functions of ${\\cal N}=1$ SQED with $N_f$ flavors, regularized by higher derivatives, by explicit calculations in the three-loop approximation. This identity explains why in the limit of the vanishing external momentum the two-point Green function of the gauge superfield is given by integrals of double total derivatives in the momentum space. It also allows to derive the NSVZ $\\beta$-function exactly in all loops if the renormalization group functions are defined in terms of the bare coupling constant. In order to verify the considered identity we use it for constructing integrals giving the three-loop $\\beta$-function starting from the two-point Green functions of the matter superfields in the two-loop approximation. Then we demonstrate that the results for these integrals coincide with the sums of the corresponding three-loop supergraphs.

  20. 4-Hydroxyestradiol induces oxidative stress and apoptosis in human mammary epithelial cells: possible protection by NF-{kappa}B and ERK/MAPK

    SciTech Connect (OSTI)

    Chen Zhihua [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of); Na, Hye-Kyung [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of); Hurh, Yeon-Jin [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of); Surh, Young-Joon [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of)]. E-mail: surh@plaza.snu.ac.kr

    2005-10-01T23:59:59.000Z

    Catechol estrogens, the hydroxylated metabolites of 17{beta}-estradiol (E{sub 2}), have been considered to be implicated in estrogen-induced carcinogenesis. 4-Hydroxyestradiol (4-OHE{sub 2}), an oxidized metabolite of E{sub 2} formed preferentially by cytochrome P450 1B1, reacts with DNA to form depurinating adducts thereby exerting genotoxicity and carcinogenicity. 4-OHE{sub 2} undergoes 2-electron oxidation to quinone via semiquinone, and during this process, reactive oxygen species (ROS) can be generated to cause DNA damage and cell death. In the present study, 4-OHE{sub 2} was found to elicit cytotoxicity in cultured human mammary epithelial (MCF-10A) cells, which was blocked by the antioxidant trolox. MCF-10A cells treated with 4-OHE{sub 2} exhibited increased intracellular ROS accumulation and 8-oxo-7,8-dihydroxy-2'-deoxyguanosine formation, and underwent apoptosis as determined by poly(ADP-ribose)polymerase cleavage and disruption of mitochondrial transmembrane potential. The redox-sensitive transcription factor nuclear factor {kappa}B (NF-{kappa}B) was transiently activated by 4-OHE{sub 2} treatment. Cotreatment of MCF-10A cells with the NF-{kappa}B inhibitor, L-1-tosylamido-2-phenylethyl chloromethyl ketone, exacerbated 4-OHE{sub 2}-induced cell death. 4-OHE{sub 2} also caused transient activation of extracellular signal-regulated protein kinases (ERK) involved in transmitting cell survival or death signals. A pharmacological inhibitor of ERK aggravated the 4-OHE{sub 2}-induced cytotoxicity, supporting the pivotal role of ERK in protecting against catechol estrogen-induced oxidative cell death.

  1. Nature of Roberge-Weiss transition end points for heavy quarks in $N_f=2$ lattice QCD with Wilson fermions

    E-Print Network [OSTI]

    Liang-Kai Wu; Xiang-Fei Meng

    2014-10-26T23:59:59.000Z

    The phase structure of QCD with imaginary chemical potential provides information on the phase diagram of QCD with real chemical potential. With imaginary chemical potential $i\\mu_I=i\\pi T$, previous studies show that the Roberge-Weiss (RW) transition end points are triple points at both large and small quark masses, and second order transition points at intermediate quark masses. The triple and second order end points are separated by two tricritical ones. We present simulations with $ N_f=2 $ Wilson fermions to investigate the nature of RW transition end points. The simulations are carried out at 8 values of the hopping parameter $\\kappa$ ranging from 0.020 to 0.140 on different lattice volumes. The Binder cumulant, susceptibility and reweighted distribution of the imaginary part of Polyakov loop are employed to determine the nature of RW transition end points. The simulations show that the two tricritical points are within the range $0.070-0.080$ and $0.120-0.140$, respectively.

  2. Measurement of the F2n/F2p and d/u Ratios in Deep Inelastic Electron Scattering off 3H and 3He

    E-Print Network [OSTI]

    G. G. Petratos; I. R. Afnan; F. Bissey; J. Gomez; A. T. Katramatou; W. Melnitchouk; A. W. Thomas

    2000-10-19T23:59:59.000Z

    We discuss a possible measurement of the ratio of the nucleon structure functions, F2n/F2p, and the ratio of the up to down quark distributions, u/d, at large x, by performing deep inelastic electron scattering from the 3H and 3He mirror nuclei with the 11 GeV upgraded beam of Jefferson Lab. The measurement is expected to be almost free of nuclear effects, which introduce a significant uncertainty in the extraction of these two ratios from deep inelastic scattering off the proton and deuteron. The results are expected to test perturbative and non-perturbative mechanisms of spin-flavor symmetry breaking in the nucleon, and constrain the structure function parameterizations needed for the interpretation of high energy e-p, p-p and p-pbar collider data. The precision of the expected data can also allow for testing competing parameterizations of the nuclear EMC effect and provide valuable constraints on models of its dynamical origin.

  3. Lattice NRQCD study of S- and P-wave bottomonium states in a thermal medium with $N_f=2+1$ light flavors

    E-Print Network [OSTI]

    Seyong Kim; Peter Petreczky; Alexander Rothkopf

    2014-09-12T23:59:59.000Z

    We investigate the properties of S-and P-wave bottomonium states in the vicinity of the deconfinement transition temperature. The light degrees of freedom are represented by dynamical lattice QCD configurations of the HotQCD collaboration with $N_f = 2 + 1$ flavors. Bottomonium correlators are obtained from bottom quark propagators, computed in Non-Relativistic QCD (NRQCD) under the background of these gauge field configurations. The spectral functions for the $^3S_1$ ($\\Upsilon$) and $^3P_1$ ($\\chi_{b1}$) channel are extracted from the Euclidean time correlators using a novel Bayesian approach in the temperature region $140 {\\rm MeV} \\le T \\le 249 {\\rm MeV}$ and the results are contrasted to those from the standard Maximum Entropy Method. We find that the new Bayesian approach is far superior to the Maximum Entropy Method and enables us to study reliably the first peak in the spectral functions even under the limitations present in the finite temperature setup. We find that $\\chi_{b1}$ survives up to $T=249 {\\rm MeV}$, the highest temperature considered in our study and put stringent constraints on the size of the medium modification of $\\Upsilon$ and $\\chi_{b1}$ states.

  4. Leptonic decay constants fK, fD and fDs with Nf = 2+1+1 twisted-mass lattice QCD

    E-Print Network [OSTI]

    N. Carrasco; P. Dimopoulos; R. Frezzotti; P. Lami; V. Lubicz; F. Nazzaro; E. Picca; L. Riggio; G. C. Rossi; F. Sanfilippo; S. Simula; C. Tarantino

    2015-02-25T23:59:59.000Z

    We present a lattice QCD calculation of the pseudoscalar decay constants fK, fD and fDs performed using the gauge configurations produced by the European Twisted Mass Collaboration with Nf = 2 + 1 + 1 dynamical quarks, which include in the sea, besides two light mass degenerate quarks, also the strange and charm quarks with masses close to their values in the real world. The simulations are based on a unitary setup for the two light mass-degenerate quarks and on a mixed action approach for the strange and charm quarks. We use data simulated at three different values of the lattice spacing in the range 0.06 - 0.09 fm and at pion masses in the range 210 - 450 MeV. Our main results are: fK+ / fpi+ = 1.184 (16), fK+ = 154.4 (2.0) MeV, which incorporate the leading strong isospin breaking correction due to the up- and down-quark mass difference, and fK = 155.0 (1.9) MeV, fD = 207.4 (3.8) MeV, fDs = 247.2 (4.1) MeV, fDs / fD = 1.192 (22) and (fDs / fD) / (fK / fpi) = 1.003 (14) obtained in the isospin symmetric limit of QCD. Combined with the experimental measurements of the leptonic decay rates of kaon, pion, D- and Ds-mesons our results lead to the following determination of the CKM matrix elements: |Vus| = 0.2269 (29), |Vcd| = 0.2221 (67) and |Vcs| = 1.014 (24). Using the latest value of |Vud| from superallowed nuclear beta decays the unitarity of the first row of the CKM matrix is fulfilled at the permille level.

  5. The REMOTE SENSlNf

    Office of Legacy Management (LM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA group currentBradleyTableSelling7 AugustAFRICAN3uj: ;;IDEC. i' , i Department_''1

  6. Propofol pretreatment attenuates LPS-induced granulocyte-macrophage colony-stimulating factor production in cultured hepatocytes by suppressing MAPK/ERK activity and NF-{kappa}B translocation

    SciTech Connect (OSTI)

    Jawan, Bruno [Department of Anesthesiology and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Kao, Y.-H. [Department of Anesthesiology and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung 804, Taiwan (China); Goto, Shigeru [Department of Surgery and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Department of Surgery, Iwao Hospital, 3059-1 Kawakami, Yufuin, Oita 879-5102 (Japan); Pan, M.-C. [Department of Anesthesiology and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Lin, Y.-C.; Hsu, L.-W.; Nakano, Toshiaki; Lai, C.-Y.; Sun, C.-K.; Cheng, Y.-F. [Department of Surgery and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Tai, M.-H. [Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung 804, Taiwan (China); Department of Medical Research and Education, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Road, Kaohsiung 813, Taiwan (China)] (and others)

    2008-06-15T23:59:59.000Z

    Propofol (PPF), a widely used intravenous anesthetic for induction and maintenance of anesthesia during surgeries, was found to possess suppressive effect on host immunity. This study aimed at investigating whether PPF plays a modulatory role in the lipopolysaccharide (LPS)-induced inflammatory cytokine expression in a cell line of rat hepatocytes. Morphological observation and viability assay showed that PPF exhibits no cytotoxicity at concentrations up to 300 {mu}M after 48 h incubation. Pretreatment with 100 {mu}M PPF for 24 h prior to LPS stimulation was performed to investigate the modulatory effect on LPS-induced inflammatory gene production. The results of semi-quantitative RT-PCR demonstrated that PPF pretreatment significantly suppressed the LPS-induced toll-like receptor (TLR)-4, CD14, tumor necrosis factor (TNF)-{alpha}, and granulocyte-macrophage colony-stimulating factor (GM-CSF) gene expression. Western blotting analysis showed that PPF pretreatment potentiated the LPS-induced TLR-4 downregulation. Flow cytometrical analysis revealed that PPF pretreatment showed no modulatory effect on the LPS-upregulated CD14 expression on hepatocytes. In addition, PPF pretreatment attenuated the phosphorylation of mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and I{kappa}B{alpha}, as well as the nuclear translocation of NF-{kappa}B primed by LPS. Moreover, addition of PD98059, a MAPK kinase inhibitor, significantly suppressed the LPS-induced NF-{kappa}B nuclear translocation and GM-CSF production, suggesting that the PPF-attenuated GM-CSF production in hepatocytes may be attributed to its suppressive effect on MAPK/ERK signaling pathway. In conclusion, PPF as an anesthetic may clinically benefit those patients who are vulnerable to sepsis by alleviating sepsis-related inflammatory response in livers.

  7. D-Saccharic acid 1,4-lactone protects diabetic rat kidney by ameliorating hyperglycemia-mediated oxidative stress and renal inflammatory cytokines via NF-?B and PKC signaling

    SciTech Connect (OSTI)

    Bhattacharya, Semantee [Department of Life Sciences and Biotechnology, Jadavpur University, 188, Raja S C Mullick Road, Kolkata 700 032 (India); Manna, Prasenjit [Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata-700054 (India); Gachhui, Ratan [Department of Life Sciences and Biotechnology, Jadavpur University, 188, Raja S C Mullick Road, Kolkata 700 032 (India); Sil, Parames C., E-mail: parames@bosemain.boseinst.ac.in [Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata-700054 (India)

    2013-02-15T23:59:59.000Z

    Increasing evidence suggests that oxidative stress is involved in the pathogenesis of diabetic nephropathy (DN) and this can be attenuated by antioxidants. D-Saccharic acid 1,4-lactone (DSL) is known for its detoxifying and antioxidant properties. Our early investigation showed that DSL can ameliorate alloxan (ALX) induced diabetes mellitus and oxidative stress in rats by inhibiting pancreatic ?-cell apoptosis. In the present study we, therefore, investigated the protective role of DSL against renal injury in ALX induced diabetic rats. ALX exposure (at a dose of 120 mg/kg body weight, i. p., once) elevated the blood glucose level, serum markers related to renal injury, the production of reactive oxygen species (ROS), and disturbed the intra-cellular antioxidant machineries. Oral administration of DSL (80 mg/kg body weight) restored all these alterations close to normal. In addition, DSL could also normalize the aldose reductase activity which was found to increase in the diabetic rats. Investigating the mechanism of its protective activity, we observed the activation of different isoforms of PKC along with the accumulation of matrix proteins like collagen and fibronectin. The diabetic rats also showed nuclear translocation of NF-?B and increase in the concentration of inflammatory cytokines in the renal tissue. The activation of mitochondria dependent apoptotic pathway was observed in the diabetic rat kidneys. However, treatment of diabetic rats with DSL counteracted all these changes. These findings, for the first time, demonstrated that DSL could ameliorate renal dysfunction in diabetic rats by suppressing the oxidative stress related signalling pathways. - Highlights: ? Sustained hyperglycemia and oxidative stress lead to diabetic renal injury. ? D-saccharic acid 1,4-lactone prevents renal damage in alloxan-induced diabetes. ? It restores intra-cellular antioxidant machineries and kidney apoptosis. ? DSL reduces hyperglycemia-mediated oxidative stress via NF-kB and PKC signaling. ? DSL may act as a beneficial agent in hyperglycemia induced renal disorder.

  8. Quine's NF---60 years on Thomas Forster

    E-Print Network [OSTI]

    Forster, T.E.

    twentieth century mathematicians used the expression ``The Crisis in Foundations''. This crisis had many in the form of Russell's paradox, appropriately in the heart of set theory. At first blush one might think

  9. Cloning and expression of equine NF-kB2

    E-Print Network [OSTI]

    Mirhosseini, Negin

    2009-05-15T23:59:59.000Z

    ., 2005). EIAV is transmitted mechanically by either an insect vector or an infected needle. EIAV causes a persistent infection which is typically classified in three defined stages: acute, chronic, and long-term asymptomatic (Leroux et al., 2001...). Initial exposure of an animal to a virulent strain usually results in an acute disease (Leroux et al., 2004; Maury, 1998). The acute stage of disease usually resolves within a few days 1...

  10. NF kappa B expression and matrix metalloproteinase activity in hypertension

    E-Print Network [OSTI]

    Wu, Kwan-I

    2009-01-01T23:59:59.000Z

    J. Silica attenuates hypertension in Lyon hypertensive rats.to experimental hypertension. J. Exp. Med. 115(6):1173-1190,Hypertensive Rat. Hypertension 52(2):415-423, 2008. Fabunmi

  11. NF 3 , the greenhouse gas missing from Kyoto

    E-Print Network [OSTI]

    Prather, Michael J; Hsu, Juno

    2008-01-01T23:59:59.000Z

    Moreover, there is an economic incentive, a tradeoff betweenthere are economic as well as environmental incentives not

  12. Distinct functions of negative regulators of NF-kappaB

    E-Print Network [OSTI]

    Kearns, Jeffrey D.

    2009-01-01T23:59:59.000Z

    Kearns JD, Cheong R, Hoffmann A, Levchenko A. (2005) S, Werner SL, Huang CS, Hoffmann A. (2006) I?B? providesWare CF, Ghosh G, Verma IM, Hoffmann A. (2007) A fourth I?B

  13. Distinct functions of negative regulators of NF-kappaB

    E-Print Network [OSTI]

    Kearns, Jeffrey D.

    2009-01-01T23:59:59.000Z

    J Immunol 174: 983-991. Emmerich F, Theurich S, Hummel M,specific (Doerre et al, 2005; Emmerich et al, 2003; Memet etDoerre et al, 2005; Emmerich et al, 2003; Memet et al, 1999;

  14. CMRR-NF Supplemental EIS Scoping Comments | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA groupTubahq.na.gov Office of the

  15. ARM - VAP Product - aerich1nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa-AnomalousProductsabrfcabrfcprecip Documentation abrfc : XDC documentation

  16. ARM - VAP Product - aerich2nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa-AnomalousProductsabrfcabrfcprecip Documentation abrfc : XDC

  17. K&L GATES LLP 1601 K STREET, N.W.

    Broader source: Energy.gov (indexed) [DOE]

    will not enter into any other agreement or document whatsoever concerning any uses of the surface of the Leased Premises without Lessee's prior written consent, which may be...

  18. Insights into the regulation of NF-kappaB and mediation of the cellular stress response by NF-kappaB

    E-Print Network [OSTI]

    Ho, Jessica Q.

    2010-01-01T23:59:59.000Z

    Cancer Res 65, 4273-4281. Hoffmann, A. , Natoli, G. , andS.L. , Huang, C.S. , and Hoffmann, A. (2006). IkappaBepsilonK.I. , DiDonato, J.A. , Hoffmann, A. , Hardwick, J.M. , and

  19. The Aoki phase for N_f=2 Wilson fermions revisited

    E-Print Network [OSTI]

    Sternbeck, A; Kerler, W; Mller-Preussker, M; Stben, H

    2004-01-01T23:59:59.000Z

    We report on a numerical reinvestigation of the Aoki phase in full lattice QCD with two flavors of unimproved Wilson fermions. For zero temperature the Aoki phase can be confirmed at inverse coupling $\\beta=4.0$ and $\\beta=4.3$, but not at $\\beta=4.6$ and $\\beta=5.0$. At non-zero temperature the Aoki phase was found to exist also at $\\beta=4.6$.

  20. A partially quenched analysis of the eta-eta' system in N_f=2 QCD

    E-Print Network [OSTI]

    Neff, H; Negele, John W; Schilling, K; Lippert, Th.

    2004-01-01T23:59:59.000Z

    We report on a first, comprehensive partially quenched study of the eta-eta' problem, based on SESAM configurations on a 16^3x32 lattice at beta=5.6 QCD with two (mass degenerate) active sea quark flavours. By means of the spectral approximation of the two-loop (hairpin) diagrams, we find clear plateau formation in the effective masses which enables us both to determine the eta-eta' mass matrix and the alpha-parameter in the effective chiral Lagrangian for the flavour singlet sector, alpha=0.028 +- 0.013.

  1. Probing the Aoki phase with N_f=2 Wilson fermions at finite temperature

    E-Print Network [OSTI]

    Ilgenfritz, E M; Mller-Preussker, M; Sternbeck, A; Stben, H

    2005-01-01T23:59:59.000Z

    In this letter we report on a numerical investigation of the Aoki phase in the case of finite temperature which continues our former study at zero temperature. We have performed simulations with Wilson fermions at $\\beta=4.6$ using lattices with temporal extension $N_{\\tau}=4$. In contrast to the zero temperature case, the existence of an Aoki phase can be confirmed for a small range in $\\kappa$ at $\\beta=4.6$, however, shifted slightly to lower $\\kappa$. Despite fine-tuning $\\kappa$ we could not separate the thermal transition line from the Aoki phase.

  2. NF-[kappa]B RelB/p52 heterodimer : biogenesis and DNA recognition

    E-Print Network [OSTI]

    Fusco, Amanda J.

    2007-01-01T23:59:59.000Z

    derived factor-1 (SDF-1), B lymphocyte chemoattractant (BLC)Sequence Consensus ELC BLC SDF-1 New Consensus for RelB/p52from the CD39, BLC, ELC and SDF-1 promoters (Figure 3-21).

  3. Gersende Prior NF-FE Meeting November 10th Neutrino Factory

    E-Print Network [OSTI]

    McDonald, Kirk

    long Hg target in 20 T field (SPL+accumulator & compressor ring). Decay: 30 m long in 1.8 T. Rotation the relations: = = = 0 (can we ?) Problems: det(0 ) = 0 2x2 sub to understand the problems or change to a "real" optics code (MAD-X, OPTIM, PATH). - pros: ICOOL not designed

  4. www.nasa.gov/aeronautics NF-2013-05-565-HQ

    E-Print Network [OSTI]

    , emissions and fuel burn. A greener future for aviation holds the promise of new life for airports, because Headquarters 300 E. Street, SW Washington, DC 20546 NASA Facts Green Aviation: A Better Way to Treat the Planet engine emissions, unprecedented traffic jams in the sky, nonstop noise near airports, and more citizens

  5. Effects of water chemistry on NF/RO membrane structure and performance

    E-Print Network [OSTI]

    Mo, Yibing

    2013-01-01T23:59:59.000Z

    contaminants by a membrane bioreactorgranular activatedA combination of a membrane bioreactor (MBR) and GAC for

  6. Effects of water chemistry on NF/RO membrane structure and performance

    E-Print Network [OSTI]

    Mo, Yibing

    2013-01-01T23:59:59.000Z

    methyl tert-butyl ether (MTBE), benzene, ethylbenzene,TCE** Industrial C 2 HCl 3 MTBE** [48] Industrial C 5 H 12 O

  7. Results of radiological measurements taken in the Niagara Falls, New York, area (NF002)

    SciTech Connect (OSTI)

    Williams, J.K.; Berven, B.A.

    1986-11-01T23:59:59.000Z

    The results of a radiological survey of 100 elevated gamma radiation anomalies in the Niagara Falls, New York, area are presented. These radiation anomalies were identified by a mobile gamma scanning survey during the period October 3-16, 1984, and were recommended for an onsite survey to determine if the elevated levels of radiation may be related to the transportation of radioactive waste material to the Lake Ontario Ordnance Works for storage. In this survey, radiological measurements included outdoor gamma exposure rates at 1 m above the surface; outdoor gamma exposure rates at the surface, range of gamma exposure rates during scan; and uranium, radium, and thorium concentrations in biased surface soil samples. The results show 38 anomalies (35 located along Pletcher Road and 3 associated with other unreleated locations) were found to exceed Formerly Utilized Sites Remedial Action Program (FUSRAP) remedial action guidelines and were recommended for formal characterization surveys. (Since the time of this survey, remedial actions have been conducted on the 38 anomalies identified as exceeding FUSRAP guidelines, and the radioactive material above guidelines has been removed.) The remaining 62 anomalies are associated with asphalt driveways and parking lots, which used a phosphate slag material (previously identified as cyclowollastonite, synthetic CaSiO/sub 3/). This rocky-slag waste material was used for bedding under asphalt surfaces and in general gravel applications. Most of the contaminated soil and rock samples collected at the latter anomalies had approximately equal concentrations of /sup 226/Ra and /sup 238/U and, therefore, are not related to materials connected with the Niagara Falls Storage Site (NFSS), including material that was transported to the NFSS. 13 refs., 7 figs., 14 tabs.

  8. University Square Stratford 1 Salway Road, Stratford, London, United Kingdom, E15 1NF

    E-Print Network [OSTI]

    Cocea, Mihaela

    has fully automatic heating, ventilation and lighting controls which require no manual operation. See. There is no smoking permitted in the premises or grounds area of the USS Building including the garden. #12;4 2

  9. Effects of water chemistry on NF/RO membrane structure and performance

    E-Print Network [OSTI]

    Mo, Yibing

    2013-01-01T23:59:59.000Z

    regulated some PAHs in the drinking water, but only Benzo-a-water of7 ppb, 700 ppb, 1ppm, 10 ppm, respectively [45]. PAHs

  10. Identification of NF-kappaB responsive elements in follistatin related gene (FLRG) promoter

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    : electrophoresis mobility-shift assay FLRG: follistatin related gene FSRP: follistatin-related protein FSTL3 lymphocytic leukemia (B-CLL). FLRG (alternative names: follistatin- related protein, FSRP / follistatin-like-3

  11. A NF-kappaB temporal code to ensure specificity in inflammatory signaling

    E-Print Network [OSTI]

    Werner, Shannon L.

    2009-01-01T23:59:59.000Z

    Kearns, J. , Cheong, R. , Hoffmann, A. , and Levchenko, A.G. , Verma, I.M. , and Hoffmann, A. 2007. A fourth IkappaBWerner, S.L. , Regal, J. , Hoffmann, A. , and Levchenko, A.

  12. Temporal encoding and homeostatic control in the IKK- kappaNF-kappaB signaling system

    E-Print Network [OSTI]

    Barken, Derren

    2007-01-01T23:59:59.000Z

    Nature 415(6868): 180-3. Hoffmann, A. and D. Baltimore (Immunol Rev 210: 171-86. Hoffmann, A. , A. Levchenko, etScience 298(5596): 1241-5. Hoffmann, A. , G. Natoli, et

  13. akt-dependent nf-kappab activation: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    particles, which take energy from an internal replenishable energy depot or ambient medium and transduce it into useful work performed on the environment, in addition to...

  14. Review of NF-IDS Targets Work Programme Status C. Densham, RAL, 17 November 2009

    E-Print Network [OSTI]

    McDonald, Kirk

    and services ? e) Cost of mercury system ? f) Cost of remote handling systems including shielding required ? g & containment TD Stalled c) Studies of irradiation, heat loads and cooling of decay solenoid JB, PL Underway d) Heat loads and cooling of shielding PL Stalled e) Solenoid system engineering, magnetic loads PL

  15. attenuates nf-kappab-dependent icam-1: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and borehole acoustic models. The validity of the scheme is established using a 3D homogenous isotropic ... Krasovec, Mary L. 2003-01-01 29 THE ATTENUATED RAY TRANSFORM...

  16. Technical Support Document: Development of the Advanced Energy Design Guide for Grocery Stores--50% Energy Savings

    SciTech Connect (OSTI)

    Hale, E. T.; Macumber, D. L.; Long, N. L.; Griffith, B. T.; Benne, K. S.; Pless, S. D.; Torcellini, P. A.

    2008-09-01T23:59:59.000Z

    This report provides recommendations that architects, designers, contractors, developers, owners, and lessees of grocery store buildings can use to achieve whole-building energy savings of at least 50% over ASHRAE Standard 90.1-2004.

  17. Oil and Gas- Leases to remove or recover (Pennsylvania)

    Broader source: Energy.gov [DOE]

    This act states that a lease or agreement conveying the right to remove or recover oil, natural gas or gas of any other designation from lessor to lessee shall not be valid if such lease does not...

  18. Leasing Texas Rangelands

    E-Print Network [OSTI]

    White, Larry D.; Whitson, Robert E.

    1996-10-25T23:59:59.000Z

    Leasing rangeland for a variety of uses can be a viable alternative to operating the enterprise yourself. This publication gives the landowner sensible guidelines for negotiating a lease, and includes discussions on lessor/lessee objectives....

  19. Oil and Gas Wells: Regulatory Provisions (Kansas)

    Broader source: Energy.gov [DOE]

    It shall be unlawful for any person, firm or corporation having possession or control of any natural gas well, oil well or coalbed natural gas well, whether as a contractor, owner, lessee, agent or...

  20. Third-Party Finance for Commercial Photovoltaic Systems: The Rise of the PPA

    E-Print Network [OSTI]

    Bolinger, Mark A

    2009-01-01T23:59:59.000Z

    lessee/site host. C. Power Purchase Agreement (PPA) Underthrough what is known as a power purchase agreement (PPA). 9from voluntary green power purchases, but to date, the price

  1. A numerical reinvestigation of the Aoki phase with N_f=2 Wilson fermions at zero temperature

    E-Print Network [OSTI]

    Ilgenfritz, E M; Mller-Preussker, M; Sternbeck, A; Stben, H

    2003-01-01T23:59:59.000Z

    We report on a numerical reinvestigation of the Aoki phase in lattice QCD with two flavors of Wilson fermions where the parity-flavor symmetry is spontaneously broken. For this purpose an explicitly symmetry-breaking source term $h\\bar{\\psi} i \\gamma_{5} \\tau^{3}\\psi$ was added to the fermion action. The order parameter $$ was computed with the Hybrid Monte Carlo algorithm at several values of $(\\beta,\\kappa,h)$ on lattices of sizes $4^4$ to $12^4$ and extrapolated to $h=0$. The existence of a parity-flavor breaking phase can be confirmed at $\\beta=4.0$ and $4.3,$ while we do not find parity-flavor breaking at $\\beta=4.6$ and 5.0.

  2. Vector and scalar form factors for K- and D-meson semileptonic decays from twisted mass fermions with Nf = 2

    E-Print Network [OSTI]

    S. Di Vita; B. Haas; F. Mescia; V. Lubicz; S. Simula; C. Tarantino

    2009-10-26T23:59:59.000Z

    We present lattice results for the form factors relevant in the K -> pion and D -> pion semileptonic decays, obtained from simulations with two flavors of dynamical twisted-mass fermions and pion masses as light as 260 MeV. For K -> pion decays we discuss the estimates of the main sources of systematic uncertainties, including the quenching of the strange quark, leading to our final result f+(0) = 0.9560 (57) (62). Combined with the latest experimental data, our value of f+(0) implies for the CKM matrix element |Vus| the value 0.2267 (5) (20) consistent with the first-row CKM unitarity. For D -> pion decays the application of Heavy Meson Chiral Perturbation Theory allows to extrapolate our results for both the scalar and the vector form factors at the physical point with quite good accuracy, obtaining a nice agreement with the experimental data. In particular at zero-momentum transfer we obtain f+(0) = 0.64 (5).

  3. Modulation of host NF-[k??a?p?p?a?]B pathway by the Toxoplasma gondii secreted factor, GRA15

    E-Print Network [OSTI]

    Lu, Diana, Ph. D. Massachusetts Institute of Technology

    2013-01-01T23:59:59.000Z

    The apicomplexan protozoan Toxoplasma gondii is an obligate intracellular pathogen that infects all warm blooded animals, including nearly thirty percent of the human population worldwide. Toxoplasma's success as a parasite ...

  4. On the Use of Thermal NF3 as the Fluorination and Oxidation Agent in Treatment of Used Nuclear Fuels

    SciTech Connect (OSTI)

    Scheele, Randall D.; McNamara, Bruce K.; Casella, Andrew M.; Kozelisky, Anne E.

    2012-05-01T23:59:59.000Z

    This paper presents results of our investigation on the use of nitrogen trifluoride as the fluorination or fluorination/oxidation agent for use in a process for separating valuable constituents from used nuclear fuels by employing the volatility of many transition metal and actinide fluorides. Nitrogen trifluoride is less chemically and reactively hazardous than the hazardous and aggressive fluorinating agents used to prepare uranium hexafluoride and considered for fluoride volatility based nuclear fuels reprocessing. In addition, nitrogen trifluorides less aggressive character may be used to separate the volatile fluorides from used fuel and from themselves based on the fluorination reactions temperature sensitivity (thermal tunability) rather than relying on differences in sublimation/boiling temperature and sorbents. Our thermodynamic calculations found that nitrogen trifluoride has the potential to produce volatile fission product and actinide fluorides from candidate oxides and metals. Our simultaneous thermogravimetric and differential thermal analyses found that the oxides of lanthanum, cerium, rhodium, and plutonium fluorinated but did not form volatile fluorides and that depending on temperature volatile fluorides formed from the oxides of niobium, molybdenum, ruthenium, tellurium, uranium, and neptunium. We also demonstrated near-quantitative removal of uranium from plutonium in a mixed oxide.

  5. Studies of resonantly produced plasmas in the H-1NF heliac using a far-infrared scanning interferometer

    E-Print Network [OSTI]

    Howard, John

    with an electric motor to provide a stable computer controlled rotational speed and to make the system acoustically and the other as a vibration monitor to compensate for wheel induced vibrations. With the grating wheel rotating and signal to noise ratio. We typically choose N 30. A Fourier shifting algorithm is used to correct

  6. QCD thermodynamics with $N_f=2+1$ near the continuum limit at realistic quark masses

    E-Print Network [OSTI]

    Takashi Umeda

    2006-10-03T23:59:59.000Z

    We report on our study of QCD thermodynamics with 2+1 flavors of dynamical quarks. In this proceeding we present several thermodynamic quantities and our recent calculation of the critical temperature. In order to investigate the thermodynamic properties of QCD near the continuum limit we adopt improved staggered (p4) quarks coupled with tree-level Symanzik improved glue on $N_t=4$ and 6 lattices. The simulations are performed with a physical value of the strange quark mass and light quark masses which are in the range of $m_q/m_s=0.05-0.4$. The lightest quark mass corresponds to a pion mass of about 150 MeV.

  7. I kappa B epsilon provides negative feedback to control NF-kappa B oscillations, signaling dynamics, and inflammatory gene expression

    E-Print Network [OSTI]

    Kearns, Jeffrey D; Basak, Soumen; Werner, Shannon L; Huang, Christine S; Hoffmann, Alexander

    2006-01-01T23:59:59.000Z

    Cancer Lett. 206: 193-199. Hoffmann, A. , and D. Baltimore.Immunol. Rev. 210:171-186. Hoffmann, A. , A. Levchenko, M.L.Science. 298:12411245. Hoffmann, A. , T.H. Leung, and D.

  8. P105 and p100 proteins function as the core of heterogeneous NF-kappaB complexes

    E-Print Network [OSTI]

    Savinova, Olga V.

    2009-01-01T23:59:59.000Z

    the p105 mutant protein that lacks a death domain, p105(1-and p105 mutant protein that lacks the C-terminal deaththat YFP-tagged p105 proteins that lack the ANK domain are

  9. I kappa B epsilon provides negative feedback to control NF-kappa B oscillations, signaling dynamics, and inflammatory gene expression

    E-Print Network [OSTI]

    Kearns, Jeffrey D; Basak, Soumen; Werner, Shannon L; Huang, Christine S; Hoffmann, Alexander

    2006-01-01T23:59:59.000Z

    Immunol. 174:983991. Emmerich, F. , S. Theurich, M. Hummel,Spiecker et al. , 2000; Emmerich et al. , 2003; Doerre etSpiecker et al. , 2000; Emmerich et al. , 2003; Doerre et

  10. ineralization of acid and sweet whey. There would be about 150 000200 000 m2 of NF

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    properties [13]. In crossflow filtration of whey using organic membranes, the most important fouling to fouling. Some studies have demonstrated that the hydrodynamic operating conditions of mem- brane crossflow

  11. A R T I C L E p53 stabilization is decreased upon NF B activation: A role for

    E-Print Network [OSTI]

    Wahl, Geoffrey M.

    et al., 1995). Since transcrip-et al., 1996), and hypoxia (Graeber et al., 1994), lead to activation

  12. MODULATION OF THE NF-KAPPA B SIGNALING PATHWAY BY THE BACTERIAL TYPE III SECRETION SYSTEM EFFECTORS

    E-Print Network [OSTI]

    Gao, Xiaofei

    2012-08-31T23:59:59.000Z

    The type III secretion system (T3SS) is a bacterial injection system expressed by many Gram-negative bacteria. During the last two decades, the repertoire of T3SS effectors has been greatly explored, and several mechanisms ...

  13. Nucleon form factors and structure functions with N_f=2+1 dynamical domain wall fermions

    E-Print Network [OSTI]

    RBC-UKQCD Collaborations; :; Takeshi Yamazaki; Shigemi Ohta

    2007-10-02T23:59:59.000Z

    We report isovector form factors and low moments of structure functions of nucleon in numerical lattice quantum chromodynamics (QCD) from the on-going calculations by the RIKEN-BNL-Columbia (RBC) and UKQCD Collaborations with (2+1) dynamical flavors of domain-wall fermion (DWF) quarks. We calculate the matrix elements with four light quark masses, corresponding to pion mass values of m_\\pi = 330-670 MeV, while the dynamical strange mass is fixed at a value close to physical, on (2.7 fm)^3 spatial volume. We found that our axial charge, g_A, at the lightest mass exhibits a large deviation from the heavier mass results. This deviation seems to be a finite-size effect as the g_A value scales with a single parameter, m_\\pi L, the product of pion mass and linear spatial lattice size. The scaling is also seen in earlier 2-flavor dynamical DWF and Wilson quark calculations. Without this lightest point, the three heavier mass results show only very mild mass dependence and linearly extrapolate to g_A=1.16(6). We determined the four form factors, the vector (Dirac), induced tensor (Pauli), axial vector and induced pseudoscalar, at a few finite momentum transfer values as well. At the physical pion mass the form-factors root mean square radii determined from the momentum-transfer dependence %of the form factors are 20-30% smaller than the corresonding experiments. The ratio of the isovector quark momentum to helicity fractions, _{u-d}/_{\\Delta u - \\Delta d} is in agreement with experiment without much mass dependence including the lightest point. We obtain an estimate, 0.81(2), by a constant fit. Although the individual momentum and helicity fractions are yet to be renormalized, they show encouraging trend toward experiment.

  14. Functions of receptor activator of NF-?B ligand (RANKL) and its receptors, RANK and OPG, are evolutionarily conserved

    E-Print Network [OSTI]

    Sutton, Kate Maurice

    2014-11-28T23:59:59.000Z

    The tumour necrosis factor (TNF) superfamily is a group of cytokines that orchestrate a variety of functions, both in the development of the architecture of immune organs and of the immune response. The mammalian TNF ...

  15. DETEW!INATION OF THE NEUTRON MAGNETIC FlONENT G.L. Greene and N.F. Ramsey

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power Administration wouldDECOMPOSITION OF CALCIUM SULFATE: A REVIEW OF THEDETEW!INATION OF THE

  16. Tax advantages of a deferred minimum annual royalty provision in oil and gas leases

    SciTech Connect (OSTI)

    Martin, R.B. Jr.

    1981-12-01T23:59:59.000Z

    This article describes how a deferred minimum annual royalty provision can serve to meet conflicting economic demands without adverse tax consequences. A deferred minimum annual royalty provision is an economic hybrid of a production royalty and a lease bonus. To a lessor, it has the advantage of guaranteeing a minimum return without regard to production. It should also encourage prompt development of the lease since a lessee will desire to void incurring subsequent minimum annual royalties on unproductive acreage. To an accrual-basis lessee, it has greater tax advantages than a lease bonus because it is deductible in the year paid or incurred. Although it exposes the lessee to more economic risk, this risk can be reduced through proper planning. In appropriate circumstances, a deferred minimum annual-royalty provision may therefore be worthy of consideration by either or both parties in an oil- and gas-leasing transaction. 44 references.

  17. Purdue University Cooperative Extension Service West Lafayette, Indiana Agricultural

    E-Print Network [OSTI]

    Agricultural Legal Affairs Introduction Besides the discovery of new accumulations, changes in the price of oil the rights to oil and gas exploration and produc- tion. Granting of rights to test-drill and to produce oil and gas is an important transfer of property rights. One might say that the oil and gas producer/ lessee

  18. MSU Extension Publication Archive Archive copy of publication, do not use for current recommendations. Up-to-date

    E-Print Network [OSTI]

    drainage loss; usually requir- ing that a new well be drilled to prevent such drainage by an adja- cent. Offset Wells It is one thing to get land leased, but quite another thing to initiate drilling, it is ob- viously cheaper for the lessee and the oil company he represents to drill as few wells

  19. Economic Recovery Tax Act of 1981: its effect on negotiating oil lease bonus payments

    SciTech Connect (OSTI)

    Nixon, C.; Fambrough, J.

    1982-09-01T23:59:59.000Z

    The Economic Recovery Tax Act of 1981, with its declining individual income tax rates, has made it more attractive in many cases for both the lessor (landowner) and lessee (oil and gas company) to have bonus lease payments paid and received on the installment basis rather than as a lump-sum. While the reduction in the maximum individual tax rate from 70 percent to 50 percent reduces some of the advantages of installment reporting, many moderate income lessors should still be able to benefit by this strategy. Both the lessor and lessee can utilize equations (1) and (2) when negotiating oil lease bonus payments to maximize their after-tax returns. The equations are flexible enough to allow for analysis of delays rentals, advance royalties, and minimum royalties as well. Since the after-tax discount rate has a significant impact on the resulting present values obtained, caution should be used in determining both the lessor's and lessee's rates. If the lessor and lessee have different discount rates due to alternative investment opportunities, the relative benefits of the lump-sum versus installment strategy may change. (JMT)

  20. You've leased your land, but haven't been paid! Now what?

    E-Print Network [OSTI]

    Boyer, Elizabeth W.

    the lessee 60 to 90 days to pay. (This allows them time to complete a title search.) If you were working the most sense. You entered into a contact. They haven't fulfilled their obligations, so now is the time outlining the problem and state the terms of the lease: they have 30 or 60 (typically) days to make payment

  1. /7 soF'/t,,,,n,re D toPm NT". Using a Co nf=guration Management Tool to CoordInate

    E-Print Network [OSTI]

    Grinter, Rebecca Elizabeth

    for coordination they provide, and their role in supporting a model of work. KEYWORDS: configuration management (CM of coordinating group work. This particular technology, a configuration management (CM) tool, incorporates begins with a description of configuration management, and how it involves coordinating the work

  2. Form factors of the $D \\to ?$ and $D \\to K$ semileptonic decays with $N_f = 2$ twisted mass lattice QCD

    E-Print Network [OSTI]

    S. Di Vita; B. Haas; V. Lubicz; F. Mescia; S. Simula; C. Tarantino; for the ETM Collaboration

    2011-04-05T23:59:59.000Z

    We present lattice results for the vector and scalar form factors of the semileptonic decays D -> \\pi \\ell \

  3. Regulation of cytochrome P450 3A4 gene expression through modulating pregnane X receptor transcriptional activity by NF-? aryl hydrocarbon receptor and xenobiotics

    E-Print Network [OSTI]

    Gu, Xinsheng

    2009-05-15T23:59:59.000Z

    Cytochrome P450 3A4 (CYP3A4) is a key enzyme responsible for the metabolism of drugs and endogenous compounds in human liver and intestine. CYP3A4 gene expression is mainly regulated by Pregnane X receptor (PXR) which is a ligand-dependent nuclear...

  4. Distinct NF-kB activation pathways engaged by T-cell receptor and co-receptor CD28 on T-cells

    E-Print Network [OSTI]

    Thaker, Youg Raj; Rudd, Christopher E

    2015-03-02T23:59:59.000Z

    at the pro-T3 stage [16]. In addition to this, Jurkat T-cell mutant lines lacking either SLP-76 or LAT expression showed a vital requirement of both molecules in mediating TCR-induced PLC?1 phosphorylation, extracellular signal-regulated kinase (ERK...

  5. DU145 human prostate cancer cells express functional Receptor Activator of NF-B: New insights in the prostate cancer bone metastasis process.

    E-Print Network [OSTI]

    Boyer, Edmond

    in the prostate cancer bone metastasis process. Mori K.1, 2, * , Le Goff B. 1, 2 , Charrier C. 1, 2 , Battaglia S cells, thus facilitating prostate cancer metastasis development in bone. We confirm that RANKL is a factor that facilitates metastasis to bone by acting as an activator of both osteoclasts and RANK

  6. Regulation of cytochrome P450 3A4 gene expression through modulating pregnane X receptor transcriptional activity by NF-? aryl hydrocarbon receptor and xenobiotics

    E-Print Network [OSTI]

    Gu, Xinsheng

    2009-05-15T23:59:59.000Z

    by biotransformation alters its biological effects, not only its pharmacokinetic behavior. In the case of pharmacology, some drugs must undergo biotransformation to exert their pharmacodynamic effect. In the case of toxicology, many xenobiotics must undergo...

  7. port reduces NF to 3.76 dB, which is in good agreement with the noise figure predicted without circuit losses at 5.8 GHz.

    E-Print Network [OSTI]

    Jerby, Eli

    monolithic low noise amplifier for wireless applications at 5 GHz, 1996 IEEE MMMICs Dig, pp 8184. 9. I and procedures utilized to design an amplifier that can simultaneously provide high-gain, low-power consumption amplifier for low cost 1.58 GHz commercial applications, 1993 IEEE MMICS Dig, pp 1316. 6. K.R. Gioffi

  8. TWIST1 associates with NF-kappaB subunit RELA via carboxyl-terminal WR domain to promote cell autonomous invasion through IL8 production

    E-Print Network [OSTI]

    2012-01-01T23:59:59.000Z

    subunit RELA via carboxyl-terminal WR domain to promote cellthrough the TWIST1 carboxy-terminal WR (Trp-Arg) domainis dependent on the C- terminal WR domain, through which

  9. Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK/JNK-dependent activation of NF-jB in THP-1 cells

    E-Print Network [OSTI]

    Lee, Won-Ha

    Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK line THP-1 (which expresses both Fas and FasL) was stimulated through treatment with agonistic anti in macrophage function, the human macrophage-like cell line THP-1 was analyzed after treatment with agonistic

  10. Three-gluon running coupling from lattice QCD at $N_f=2+1+1$: a consistency check of the OPE approach

    E-Print Network [OSTI]

    Ph. Boucaud; M. Brinet; F. De Soto; V. Morenas; O. Pne; K. Petrov; J. Rodrguez-Quintero

    2014-01-31T23:59:59.000Z

    We present a lattice calculation of the renormalized running coupling constant in symmetric (MOM) and asymmetric ($\\widetilde{\\rm MOM}$) momentum substraction schemes including $u$, $d$, $s$ and $c$ quarks in the sea. An Operator Product Expansion dominated by the dimension-two $\\langle A^2\\rangle$ condensate is used to fit the running of the coupling. We argue that the agreement in the predicted $\\langle A^2\\rangle$ condensate for both schemes is a strong support for the validity of the OPE approach and the effect of this non-gauge invariant condensate over the running of the strong coupling.

  11. 15-Deoxy-?{sup 12,14}-prostaglandin J{sub 2} inhibits IL-13 production in T cells via an NF-?B-dependent mechanism

    SciTech Connect (OSTI)

    Doyle, Marie-Christine; Tremblay, Sarah [Dpartement de Biologie, Facult des Sciences, Universit de Sherbrooke, Sherbrooke (QC), Canada J1K 2R1 (Canada)] [Dpartement de Biologie, Facult des Sciences, Universit de Sherbrooke, Sherbrooke (QC), Canada J1K 2R1 (Canada); Dumais, Nancy, E-mail: nancy.dumais@usherbrooke.ca [Dpartement de Biologie, Facult des Sciences, Universit de Sherbrooke, Sherbrooke (QC), Canada J1K 2R1 (Canada)] [Dpartement de Biologie, Facult des Sciences, Universit de Sherbrooke, Sherbrooke (QC), Canada J1K 2R1 (Canada)

    2013-02-15T23:59:59.000Z

    Highlights: ? 15d-PGJ{sub 2} decreased IL-13 mRNA transcription and secretion in activated T cells. ? IL-13 inhibition by 15d-PGJ{sub 2} is independent of PPAR-?. ? The nuclear factor-?B mediates the 15d-PGJ{sub 2}-dependent down regulation of IL-13. -- Abstract: Interleukin (IL)-13 is a cytokine produced by activated CD4{sup +} T cells that plays a critical role in promoting allergic responses and tumor cell growth. The 15-deoxy-?{sup 12,14}-prostaglandin J{sub 2} (15d-PGJ{sub 2}) is a natural ligand for the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-?), a known regulator of anti-inflammatory activities. We determined the effects of 15d-PGJ{sub 2} on IL-13 expression in the Jurkat E6.1 T-cell line and in peripheral blood mononuclear cells. Semi-quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay revealed that treatment of activated T cells with 15d-PGJ{sub 2} significantly decreased IL-13 mRNA transcription and secretion, respectively. This inhibition by 15d-PGJ{sub 2} was independent of PPAR-? since treatment with GW9662, an irreversible antagonist of the nuclear receptor, produced no effect. Our data also revealed the involvement of nuclear factor-?B in mediating 15d-PGJ{sub 2}-dependent down regulation of IL-13 expression. Collectively, these results demonstrate the potential of 15d-PGJ{sub 2} in attenuating expression and production of IL-13 in activated T cells.

  12. D O M A I NF A L L 2 0 0 4 55555 How a Top Secret Satellite and Fast-Thinking

    E-Print Network [OSTI]

    Weathermen Saved the Apollo 11 Astronauts from Disaster I t was one of those turning points in history where his "one small step for man," and the Apollo 11 Moon landers were speed ing back towards Earth-thinking weathermen -- Navy CAPT Willard "Sam" Houston and Air Force Maj. Hank Brandli. Saving Apollo 11Saving Apollo

  13. Deregulation of the phosphatidylinositol-3 kinase signaling cascade is associated with neurodegeneration in Npc1-/- mouse brain.

    E-Print Network [OSTI]

    Bi, Xiaoning; Liu, Jihua; Yao, Yueqin; Baudry, Michel; Lynch, Gary

    2005-01-01T23:59:59.000Z

    Inherited Diseases Deregulation of the Phosphatidylinositol-tion, and for NF- ? B deregulation; moreover, these changes3 ? Is Associated with Deregulation of NF- ? B Accumulating

  14. Plant polyphenols differentially modulate inflammatory responses of human keratinocytes by interfering with activation of transcription factors NF{kappa}B and AhR and EGFR-ERK pathway

    SciTech Connect (OSTI)

    Potapovich, Alla I. [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Biology Department, Belarus State University, Skorina Prosp. 10, Minsk 220050 (Belarus); Lulli, Daniela; Fidanza, Paolo [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Kostyuk, Vladimir A. [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Biology Department, Belarus State University, Skorina Prosp. 10, Minsk 220050 (Belarus); De Luca, Chiara; Pastore, Saveria [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Korkina, Liudmila G., E-mail: l.korkina@idi.it [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy)

    2011-09-01T23:59:59.000Z

    Molecular mechanisms underlying modulation of inflammatory responses in primary human keratinocytes by plant polyphenols (PPs), namely the glycosylated phenylpropanoid verbascoside, the stilbenoid resveratrol and its glycoside polydatin, and the flavonoid quercetin and its glycoside rutin were evaluated. As non-lethal stimuli, the prototypic ligand for epidermal growth factor receptor (EGFR) transforming growth factor alpha (TGFalpha), the combination of tumor necrosis factor (TNFalpha) and interferon (IFNgamma) (T/I), UVA + UVB irradiation, and bacterial lipopolysaccharide (LPS) were used. We demonstrated differential modulation of inflammatory responses in keratinocytes at signal transduction, gene transcription, and protein synthesis levels as a function of PP chemical structure, the pro-inflammatory trigger used, and PP interaction with intracellular detoxifying systems. The PPs remarkably inhibited constitutive, LPS- and T/I-induced but not TGFalpha-induced ERK phosphorylation. They also suppressed NFkappaB activation by LPS and T/I. Verbascoside and quercetin invariably impaired EGFR phosphorylation and UV-associated aryl hydrocarbon receptor (AhR)-mediated signaling, while rutin, polydatin and resveratrol did not affect EGFR phosphorylation and further activated AhR machinery in UV-exposed keratinocytes. In general, PPs down-regulated gene expression of pro-inflammatory cytokines/enzymes, except significant up-regulation of IL-8 observed under stimulation with TGFalpha. Both spontaneous and T/I-induced release of IL-8 and IP-10 was suppressed, although 50 {mu}M resveratrol and polydatin up-regulated IL-8. At this concentration, resveratrol activated both gene expression and de novo synthesis of IL-8 and AhR-mediated mechanisms were involved. We conclude that PPs differentially modulate the inflammatory response of human keratinocytes through distinct signal transduction pathways, including AhR and EGFR. - Graphical abstract: Display Omitted Highlights: > Effects of plant polyphenols on inflammatory responses in human keratinocytes. > Inflammatory stimuli used: TGFalpha, TNFalpha+IFNgamma, UVA+UVB, and LPS. > Inflammatory pathways connected with NFB, ERK1/2, EGFR, and AhR were investigated. > Plant polyphenols, flavonoids, stilbenoids, and phenylpropanoids, were studied. > Modulation of inflammation depends on phenolic core structure and glycosylation.

  15. High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53 - NF-kappaB - complex-dependent gene expression in human heart failure

    E-Print Network [OSTI]

    Choy, Mun-Kit; Movassagh, Mehregan; Siggens, Lee; Vujic, Ana; Goddard, Martin; Sanchez, Ana; Perkins, Neil; Figg, Nichola; Bennett, Martin; Carroll, Jason; Foo, Roger S-Y

    2010-06-14T23:59:59.000Z

    00.5 1 1.5 2 miR-15b miR-21 miR-125b -Dox +Dox Additional file 2 (Choy, et al) 40 30 20 1 0 mir-15b mir-21 mir-125b Dox control m iR N A a bu nd an ce [a rb ita ry u ni ts ] 0 0.02 0.04 0.06 0.08 1 2 normoxia hypoxia 30 0 6 90 CF m iR -2 1 ab un... da nc e [a rb ita ry u ni ts ] Hypoxia - + WB: p53 WB: SF2 DFX - -+ + Nuc Cyt WB: p53 WB: SF2 WB: RhoGDI A B C Dox - + WB: p53 WB: SF2 * * ...

  16. Supplementary Fig. 1. Inhibition of TLR2-induced NF-B activation by TirS is comparable to inhibition mediated by DN-hMyD88 or addition of a TLR2 antagonist SSL3. Untreated control

    E-Print Network [OSTI]

    Nizet, Victor

    to inhibition mediated by DN-hMyD88 or addition of a TLR2 antagonist SSL3. Untreated control cells were

  17. antibodies targeting cancer: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of an NF-B responsive element (pNF-B- Luc) encapsulated in a PRb peptide functionalized stealth targeted systems. In addition, we constructed a novel cancer therapeutic plasmid...

  18. akt phosphorylation sites: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 36 Mass spectrometric analysis of bovine neurofilament proteins NF-L, NF-M,...

  19. Naval Petroleum Reserve No. 2: Buena Vista Oil and Gas Field, Kern County, California: Proved reserves, Developed and undeveloped, Sections 6 and 8: Development history and exploitation techniques, Effective July 1, 1987: (Final technical report)

    SciTech Connect (OSTI)

    Carey, K.B.

    1987-09-09T23:59:59.000Z

    The research for the initial Naval Petroleum Reserve No. 2 (NPR-2), study Task Assignment 010, showed the possibility of undeveloped proved reserves in the Shallow Pool on Government leases. Task Assignment 010C included a study to confirm or disprove the possibility. The six-section area, which is highlighted on Exhibit M-2, was chosen as the area for specific study of this subject. The Shallow Oil Zone, as depicted on Exhibit S-1, was the focal point of the study in the area. Competitive development of Government land with adjacent privately held land is an issue which has often been raised regarding NPR-2; however, it has never been formally addressed. Task Assignment 010C commissioned a study of the subject in the same six-section area designated for the study of proved undeveloped reserves. The producing formations in the Buena Vista Field of NPR-2 are very similar to the producing formations in the Elk Hills Field of NPR-1 to the north. It is possible that some of the successful development techniques utilized in NPR-2 by the various operators might enhance production efficiency at NPR-1. Task Assignment 010C included a detailed task of researching techniques used in NPR-2 for possible application in NPR-1. Because the detailed tasks of Task Assignment 010C are divergent in scope, a composite summary of the study's research is not included in this report. Each task's research is detailed in a separate Discussion section. Exhibits for these discussions are contained in an Exhibit section at the end of this volume. The appendices include: task assignment; DOE letters to lessees; Evans, Carey and Crozier letters to lessees; reports and studies from lessees; core analysis data; production data; geologic picks of formation tops; and annotated well logs. 22 figs., 6 tabs.

  20. Impact of geothermal development on stockraising homestead landowners

    SciTech Connect (OSTI)

    Not Available

    1981-04-16T23:59:59.000Z

    Surface use and compensation conflicts have developed at the Geysers in California between owners of surface lands acquired under the Stockraising Homestead Act of 1916 and geothermal lessees with the right to develop the mineral interests reserved to the Federal Government. Several recommendations are made to the Secretary of the Interior concerning the problems identified. The following are discussed: conditions at the Geysers concerning geothermal development on stockraising lands that could be considered in regard to compensation, existence or potential for similar conflicts on this land outside the Geysers, protection and compensation provided surface owners in existence of legislation and the need for amendments, and alternative methods for paying compensation.

  1. AZA-009172 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnualProperty Edit withTianlinPapersWindey Wind6:00-06:00 U.S.ratios inAS 42.05,AXI LLC JumpH Lessee

  2. NVN-065558 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF LAND MGMT Lessee 1

  3. NVN-065561 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF LAND MGMT Lessee

  4. NVN-079104 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee 2 none

  5. NVN-079105 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee 2 none5 Jump

  6. NVN-079106 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee 2 none5

  7. NVN-079276 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee 2 none56

  8. NVN-079291 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee 2

  9. NVN-079292 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee

  10. NVN-079305 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee7/1/2006

  11. NVN-079306 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee7/1/2006

  12. NVN-079307 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee7/1/2006 Case

  13. NVN-079310 | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to: navigation, searchOfRose BendMiasoleTremor(Question) |RenewableBUREAU OF01Lessee7/1/2006

  14. 3D Model of the McGinness Hills Geothermal Area

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Faulds, James E.

    The McGinness Hills geothermal system lies in a ~8.5 km wide, north-northeast trending accommodation zone defined by east-dipping normal faults bounding the Toiyabe Range to the west and west-dipping normal faults bounding the Simpson Park Mountains to the east. Within this broad accommodation zone lies a fault step-over defined by north-northeast striking, west-dipping normal faults which step to the left at roughly the latitude of the McGinness Hills geothermal system. The McGinness Hills 3D model consists of 9 geologic units and 41 faults. The basal geologic units are metasediments of the Ordovician Valmy and Vininni Formations (undifferentiated in the model) which are intruded by Jurassic granitic rocks. Unconformably overlying is a ~100s m-thick section of Tertiary andesitic lava flows and four Oligocene-to-Miocene ash-flow tuffs: The Rattlesnake Canyon Tuff, tuff of Sutcliffe, the Cambell Creek Tuff and the Nine Hill tuff. Overlying are sequences of pre-to-syn-extensional Quaternary alluvium and post-extensional Quaternary alluvium. 10-15 eastward dip of the Tertiary stratigraphy is controlled by the predominant west-dipping fault set. Geothermal production comes from two west dipping normal faults in the northern limb of the step over. Injection is into west dipping faults in the southern limb of the step over. Production and injection sites are in hydrologic communication, but at a deep level, as the northwest striking fault that links the southern and northern limbs of the step-over has no permeability.

  15. 3D Model of the McGinness Hills Geothermal Area

    SciTech Connect (OSTI)

    Faulds, James E.

    2013-12-31T23:59:59.000Z

    The McGinness Hills geothermal system lies in a ~8.5 km wide, north-northeast trending accommodation zone defined by east-dipping normal faults bounding the Toiyabe Range to the west and west-dipping normal faults bounding the Simpson Park Mountains to the east. Within this broad accommodation zone lies a fault step-over defined by north-northeast striking, west-dipping normal faults which step to the left at roughly the latitude of the McGinness Hills geothermal system. The McGinness Hills 3D model consists of 9 geologic units and 41 faults. The basal geologic units are metasediments of the Ordovician Valmy and Vininni Formations (undifferentiated in the model) which are intruded by Jurassic granitic rocks. Unconformably overlying is a ~100s m-thick section of Tertiary andesitic lava flows and four Oligocene-to-Miocene ash-flow tuffs: The Rattlesnake Canyon Tuff, tuff of Sutcliffe, the Cambell Creek Tuff and the Nine Hill tuff. Overlying are sequences of pre-to-syn-extensional Quaternary alluvium and post-extensional Quaternary alluvium. 10-15 eastward dip of the Tertiary stratigraphy is controlled by the predominant west-dipping fault set. Geothermal production comes from two west dipping normal faults in the northern limb of the step over. Injection is into west dipping faults in the southern limb of the step over. Production and injection sites are in hydrologic communication, but at a deep level, as the northwest striking fault that links the southern and northern limbs of the step-over has no permeability.

  16. E-Print Network 3.0 - ankyrin-repeat membrane protein Sample...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    10 Stabilizing IB by "Consensus" Design Diego U. Ferreiro1,3 Summary: Keywords: protein folding; ankyrin repeat protein; NF-B; transcription factor; repeat protein...

  17. E-Print Network 3.0 - ankyrin protein networks Sample Search...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    >> 21 Stabilizing IB by "Consensus" Design Diego U. Ferreiro1,3 Summary: Keywords: protein folding; ankyrin repeat protein; NF-B; transcription factor; repeat protein...

  18. Garden Patch Chili

    E-Print Network [OSTI]

    ... 17-ounce can corn kernels, drained Toppings of your choice (salsa, chopped red onions, chopped cilantro, nf yogurt, [avocado, cheese], etc.) 1. Drain beans.

  19. PROTON BEAM REQUIREMENTS FOR A NEUTRINO FACTORY AND MUON COLLIDER

    E-Print Network [OSTI]

    Zisman, Michael S.

    2010-01-01T23:59:59.000Z

    Table 1 summarizes the NF proton driver parameters obtainedboth facilities. Table 1. Proton driver requirements for arepetition frequency (Hz) Proton energy (GeV) Proton rms

  20. ITREOH building of regional capacity to monitor recreational water: Development of a non-commercial microcystin ELISA and its impact on public health policy

    E-Print Network [OSTI]

    2006-01-01T23:59:59.000Z

    International Center; Uruguay, Rio de la Plata; waterRepublica, Montevideo, Uruguay (BMB, LD, NF); the Laboratoryof Montev- ideo, Uruguay (BMB, DS); the National Direction

  1. Insights into the homeostatic regulation of I kappa B alpha

    E-Print Network [OSTI]

    Lisabeth, Erika Mathes

    2009-01-01T23:59:59.000Z

    Biol, 13, 1572-1582. Emmerich, F. , Meiser, M. , Hummel,fully inhibit NF-?B (Emmerich et al. , 1999; Jungnickel et

  2. amniotic membrane graft: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by grafting of Engineering Websites Summary: of polyamide thin-film composite membranes. The structure of nanofiltration (NF) and reverse osmosis (RO.V. All rights...

  3. advanced membrane filtration: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    available reverse osmosis (RO) and nanofiltration (NF) membranes are based on the thin film composite (TFC) aromatic polyamide membranes. However, they have several disadvantages...

  4. Control of size and charge selectivity in amphiphilic graft copolymer nanofiltration membranes

    E-Print Network [OSTI]

    Lovell, Nathan Gary

    2010-01-01T23:59:59.000Z

    The throughput and efficiency of membrane separations make polymer filtration membranes an important resource for the pharmaceutical, food and wastewater treatment industries. Nanofiltration (NF) membranes fill an important ...

  5. Anti-inflammatory and anti-oxidative effects of corilagin in a rat model of acute cholestasis

    E-Print Network [OSTI]

    2013-01-01T23:59:59.000Z

    MPO), malondialdehyde (MDA), superoxide dismutase (SOD) andintervention, levels of MPO, MDA and translocation of NF-?BMPO), malondialdehyde (MDA), super- oxide dismutase (SOD),

  6. air-stable planar chiral: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in QCD. One of the predictions of orientifold planar equivalence is that the chiral condensates of a theory with Nf flavours of Dirac fermions in the symmetric (or...

  7. Computing Needs Time Edward A. Lee

    E-Print Network [OSTI]

    (CSR-CPS)), the U. S. Army Research Office (ARO #W911NF-07-2-0019), the U. S. Air Force Office essential prop- erties of the physical systems, most particularly the passage of time. It makes a case (CSR-CPS)), the U. S. Army Research Office (ARO #W911NF-07-2-0019), the U. S. Air Force Office

  8. MEETING ABSTRACT Open Access Predominant role of Tax sumoylation in

    E-Print Network [OSTI]

    Boyer, Edmond

    the NF-kB pathway by acting both at cytoplasmic and nuclear levels. In the cytoplasm, Tax binds to and activates the IkB Kinase (IKK) complex while in the nucleus, Tax assembles tran- scriptional active nuclear bodies. Others and we have previously demonstrated that the cytoplasmic/nuclear partition and NF

  9. Relating Interactions between Neurofilaments to the Structure of Axonal Neurofilament Distributions through Polymer Brush Models

    E-Print Network [OSTI]

    Kumar, Sanjay

    ; Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195 with the experimental observation that sidearm phosphor- ylation regulates interfilament spacing. The presence-L, 61 kDa in humans), medium (NF-M, 90 kDa), and heavy (NF-H, 110 kDa) (Lee and Cleveland, 1996

  10. Increasing Cancer-Specific Gene Expression by Targeting Overexpressed 51 Integrin and Upregulated Transcriptional

    E-Print Network [OSTI]

    Kokkoli, Efie

    Engineering and Materials Science, University of Minnesota, 421 Washington Ave SE, Minneapolis, Minnesota the control of an NF-B responsive element (pNF-B- Luc) encapsulated in a PR_b peptide functionalized stealth was seen when transfected with PR_b functionalized stealth liposomes encapsulating the condensed p

  11. Dr. Pete's useful stuff College Physics I*

    E-Print Network [OSTI]

    Nelson, Peter Hugo

    /nelson Hooke's Law & SHM Friction NfNf kkss = kineticstatic Gravitation Quadratic functions ax2 + bx + c = 0 Momentum im= = p v P p rr rr Conservation laws for an isolated system i f i fE E P P= = simple pendulum k g

  12. Reason for Testing (Required-failure to include a diagnosis may delay testing. Include all pertinent diagnoses and ICD9 codes. Ie. Primary Lung Cancer 162.9 with metastasis to brain 198.3 and bone 198.5)

    E-Print Network [OSTI]

    Wang, David

    , NF1, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTCH1, PTEN, PTPN11, RB1, RET, RUNX1, SMO, STK11, TET2, TP53 MAP2K2 MET MPL MTOR MYC MYD88 NF1 NOTCH1 NPM1 NRAS PDGFRA PIK3CA PTCH1 PTEN PTPN11 RB1 RET RUNX1 SMO

  13. TEA: Automatically proving termination of programs in a nonstrict higherorder functional

    E-Print Network [OSTI]

    Schmidt-Schauss, Manfred

    TEA: Automatically proving termination of programs in a non­strict higher­order functional language termination of functions written in a non­strict high­level functional programming language like Haskell kinds of termination: nf­termination and lazy termination. Intuitively, nf­termination of f means

  14. Quadratic and differential forms over function fields of Pfister quadrics in characteristic two*

    E-Print Network [OSTI]

    @inst-mat.utalca.cl Abstract Let F be a field of characteristic 2. Let nFbe the F-space of differ- ential + nV a db, then let nF= 1Fand d : nF ! n+1Fbe the differential operato

  15. The BMM Global-Local bijection for GL(n,q) fhm rinivsn

    E-Print Network [OSTI]

    Srinivasan, Bhama

    prtition of nF heorem @pongErinivsnA D re in the sme `Elok if nd only if D hve the sme eEoreF Bhama of nF heorem @pongErinivsnA D re in the sme `Elok if nd only if D hve the sme eEoreF Bhama Srinivasan

  16. ENEA F. Zonca 1 Multiple space-time structure of low-frequency fluctuations in

    E-Print Network [OSTI]

    Zonca, Fulvio

    techniques based on scale separation. 2 BF introduced by a number of authors in the late 70's (Coppi PRL77, Lee PFBW 77, Glasser PFBW 77, Pegoraro IAEA 78, Connor PRL 78, Dewar NF81) to conveniently treat (Coppi PRL77, Lee PFBW 77, Glasser PFBW 77, Pegoraro IAEA 78, Connor PRL 78, Dewar NF81) to conveniently

  17. A novel derivative of decursin, CSL-32, blocks migration and production of inflammatory mediators and modulates PI3K

    E-Print Network [OSTI]

    Lee, Won-Ha

    and Biotechnology, Kyungpook National University, Daegu 702-701, Korea { College of Pharmacy, Chungnam NationalB), phosphorylation of p65 subunit of NF-kB (nuclear factor-kB) and nuclear translocation of NF-kB, which are required

  18. Dmarche qualit au sein d'un laboratoire de recherche du CNRS: FEMTO-ST

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    galement lancs dans la mise en place de la norme NF EN ISO 9001 afin d'obtenir une certification : recherche, qualit, laboratoires, label Carnot, ISO 9001, ISO/CEI 17025, Universit de Franche-Comt, FEMTO dcennies des laboratoires accrdits par le COFRAC selon le rfrentiel NF EN ISO/CEI 17025. Ainsi, le

  19. Neurofilament Proteins in Avian Auditory Hair Cells

    E-Print Network [OSTI]

    Rubel, Edwin

    Neurofilament Proteins in Avian Auditory Hair Cells ELIZABETH C. OESTERLE,* DIANA I. LURIE avian inner ear by using immunocytochemical techniques. NF-M was detected in auditory hair cells and VIIIth cranial nerve neurons. NF-M-positive hair cells are first detected at embryonic day 11 (E11

  20. ASPECTS OF PARTIAL INFORMATION IN DATABASES Leonid Libkin

    E-Print Network [OSTI]

    Libkin, Leonid

    {c7 38. Kg4{f5 Nf6:d5+ 13. h2{h3? Bc8{d7 39. Kf5{g4 Nd5{f6+ 14. Rb1{c1 g7{g6 40. Kg4{f5 Nf6{g8+ 15. Nf3{d2 Ra8{b8 41. Kf5{g4 Ng8{f6+ 16. Nd2{b3 Nc5:b3 42. Kg4{f5 Nf6{g8+ 17. Qc2:b3 c6{c5 43. Kf5{g4 Be7:g5 18. Kg1{h2 Kg8{h8 44. Kg4:g5 Rf8{f7 19. Qb3{c2 Nf6{g8 45. Bf2{h4 Rh6{g6+ 20. Be2{g4 Ng8{h6 46. Kg

  1. Technical Support Document: Development of the Advanced Energy Design Guide for Medium Box Retail -- 50% Energy Savings

    SciTech Connect (OSTI)

    Hale, E. T.; Macumber, D. L.; Long, N. L.; Griffith, B. T.; Benne, K. S.; Pless, S. D.; Torcellini, P. A.

    2008-09-01T23:59:59.000Z

    This report provides recommendations that architects, designers, contractors, developers, owners, and lessees of medium box retail buildings can use to achieve whole-building energy savings of at least 50% over ASHRAE Standard 90.1-2004. The recommendations are given by climate zone and address building envelope, fenestration, lighting systems, HVAC systems, building automation and controls, outside air treatment, service water heating, plug loads, and photovoltaic systems. The report presents several paths to 50% savings, which correspond to different levels of integrated design. These are recommendations only, and are not part of a code or standard. The recommendations are not exhaustive, but we do try to emphasize the benefits of integrated building design, that is, a design approach that analyzes a building as a whole system, rather than as a disconnected collection of individually engineered subsystems.

  2. Optimum combination of leasing systems on public lands

    SciTech Connect (OSTI)

    McDonald, S.L.

    1984-12-01T23:59:59.000Z

    Bonus bidding for oil and gas leases on public lands has been a reasonably satisfactory system for capturing the average rent available while being neutral with respect to economic decisions such as abandonment. However, it places a heavy burden of risk and uncertainty on the lessee. There is some reason to believe that social gains are to be had from shifting some of the risk and uncertainty to the lessors (governmental units). Profit-share or royalty bidding tends to accomplish such a shift. However, optimality seems to call for a combination of bonus bidding and profit-share (or royalty) bidding, the latter on wildcat tracts and the former on drainage tracts. Profit-share bidding is somewhat superior to royalty bidding, and the best definition of ''profit'' for the purpose is profits in excess of a normal return on investment. The combination suggested above may be especially desirable in a program of accelerated leasing of public lands. 2 figs.

  3. Planning techniques for avoiding sublease treatment on assigning oil and gas leases

    SciTech Connect (OSTI)

    Kells, R.B.

    1983-06-01T23:59:59.000Z

    The negative tax consequences that may occur upon transfer of a nonproducing oil and gas lease are discussed. It is usually assumed that income taxes are computed on their actual economic gain, however, taxes are computed often on income that is far greater an amount than the economic gain. Suggestionss are made for structuring the sales to obtain the most-favorable tax arrangement. It is also suggested that legislation be enacted to provide for sale treatment in the instance when a lessee assigns a lease but still retains a continuing non-operating interest. Taxing discrepancies that now exist between oil/gas properties and those of other minerals would also be lessened by such legislation. 48 references.

  4. Influence of the Torsion Angle in 3,3'-Dimethyl-2,2'-bipyridine on the Intermediate Valence of Yb in (C5Me5)2 Yb(3,3'-Me2-bipy)

    SciTech Connect (OSTI)

    Nocton, Gr& #233; gory; Booth, Corwin H.; Maron, Laurent; Andersen, Richard A.

    2014-06-26T23:59:59.000Z

    The synthesis and X-ray crystal structures of Cp-2*Yb(3,3'-Me(2)bipy) and [Cp-2 Yb(3,3'-Me(2)bipy)][Cp-2 YbCl1.6I0.4]center dot CH2Cl2 are described. In both complexes, the NCCN torsion angles are approximately 40 degrees. The temperature-independent value of n(f) of 0.17 shows that the valence of ytterbium in the neutral adduct is multiconfigurational, in reasonable agreement with a CASSCF calculation that yields a n(f) value of 0.27; that is, the two configurations in the wave function are f(13)(pi(1))(1) and f(14)(pi(1))(0) in a ratio of 0.27:0.73, respectively, and the open-shell singlet lies 0.28 eV below the triplet state (n(f) accounts for f-hole occupancy; that is, n(f) = 1 when the configuration is f(13) and n(f) = 0 when the configuration is f(14)). A correlation is outlined between the value of nf and the individual ytterbocene and bipyridine fragments such that, as the reduction potentials of the ytterbocene cation and the free x,x'-R-2-bipy ligands approach each other, the value of nf and therefore the f(13):f(14) ratio reaches a maximum; conversely, the ratio is minimized as the disparity increases.

  5. Numerical Investigation of Interaction Between Hydraulic Fractures and Natural Fractures

    E-Print Network [OSTI]

    Xue, Wenxu

    2011-02-22T23:59:59.000Z

    Hydraulic fracturing of a naturally-fractured reservoir is a challenge for industry, as fractures can have complex growth patterns when propagating in systems of natural fractures in the reservoir. Fracture propagation near a natural fracture (NF...

  6. after-school program targeting: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Karl R; Wyatt, Mark C 2015-01-01 22 KT McDonald Muon Accelerator Program DoE Review (FNAL) August 30, 2012 1 Target and Absorbers Physics Websites Summary: In the IDS-NF...

  7. ANU OPEN DAY PrOgrAm SAtUrDAY 28 AUgUSt 2010, 9am4pm

    E-Print Network [OSTI]

    -Intensive Computing Intelligent Systems iNfOrmAtiON tEChNOlOgY Information Systems Software Development SOft Business Administration Business Information Systems Corporate Sustainability economics Finance

  8. Chemical Addition prior to Membrane Processes for Natural Organic Matter (NOM) Removal

    E-Print Network [OSTI]

    Schfer, Andrea; Fane, Anthony G.; Waite, T. D.

    1998-01-01T23:59:59.000Z

    Membrane processes for surface water treatment include microfiltration (MF), ultrafiltration (UF) and nanofiltration (NF), depending on the target material to be removed and the limiting process economics. MF will remove ...

  9. Phase Resetting Reveals Network Dynamics Underlying a Bacterial Cell Cycle

    E-Print Network [OSTI]

    Scherer, Norbert F.

    promoter to express the essential transcriptional regulatory gene ctrA in a periodic, pulsed fashion perturbation spectroscopy (CPS). Citation: Lin Y, Li Y, Crosson S, Dinner AR, Scherer NF (2012) Phase Resetting

  10. Systems and methods for treating material

    DOE Patents [OSTI]

    Scheele, Randall D; McNamara, Bruce K

    2014-10-21T23:59:59.000Z

    Systems for treating material are provided that can include a vessel defining a volume, at least one conduit coupled to the vessel and in fluid communication with the vessel, material within the vessel, and NF.sub.3 material within the conduit. Methods for fluorinating material are provided that can include exposing the material to NF.sub.3 to fluorinate at least a portion of the material. Methods for separating components of material are also provided that can include exposing the material to NF.sub.3 to at least partially fluorinate a portion of the material, and separating at least one fluorinated component of the fluorinated portion from the material. The materials exposed to the NF.sub.3 material can include but are not limited to one or more of U, Ru, Rh, Mo, Tc, Np, Pu, Sb, Ag, Am, Sn, Zr, Cs, Th, and/or Rb.

  11. Critical risk points of nanofiltration and reverse osmosis processes in water recycling applications

    E-Print Network [OSTI]

    Nghiem, Long D.; Schfer, Andrea

    2006-01-01T23:59:59.000Z

    NF/RO membrane filtration processes have been recognized as an important technology to facilitate water recycling. Those processes are well-proven technologies, which can be used to remove a wide range of contaminants including trace contaminants...

  12. Dysregulation of nuclear factor kappa B activity and osteopontin expression in oxidant-induced atherogenesis

    E-Print Network [OSTI]

    Williams, Edward Spencer

    2004-09-30T23:59:59.000Z

    NF-?B activity is critical in the regulation of atherosclerotic vascular smooth muscle cell (vSMC) phenotypes induced following oxidative injury by allylamine. The present studies were designed to detail dysregulation of ...

  13. E-Print Network 3.0 - algorithms elucidate nrf2 Sample Search...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Medicine 70 Kinetics and Mechanism of Protein Tyrosine Phosphatase 1B Inactivation by Acrolein Summary: channels (TRPA1) (17), and some transcription factors (NF-B, Keap1Nrf2)...

  14. E-Print Network 3.0 - agent-sensor keap1 protein Sample Search...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Medicine 48 Kinetics and Mechanism of Protein Tyrosine Phosphatase 1B Inactivation by Acrolein Summary: channels (TRPA1) (17), and some transcription factors (NF-B, Keap1Nrf2)...

  15. Synthesis and Characterization of Novel Fluorescent Injectable Micro-Carriers for Tissue Regeneration

    E-Print Network [OSTI]

    Arora, Akshi

    2014-04-28T23:59:59.000Z

    Specific problem: Our previous study showed that the nanofibrous poly-l-lactic acid (NF-PLLA) microspheres are excellent cell carriers for tissue regeneration. However, these injectable microspheres are not fluorescent biomaterials. Incorporation...

  16. Exploration of nucleon structure in lattice QCD with chiral quarks

    E-Print Network [OSTI]

    Syritsyn, Sergey Nikolaevich

    2010-01-01T23:59:59.000Z

    In this work, we calculate various nucleon structure observables using the fundamental theory of quarks and gluons, QCD, simulated on a lattice. In our simulations, we use the full QCD action including Nf = 2+ 1 dynamical ...

  17. Monopole Taxonomy in Three-Dimensional Conformal Field Theories

    E-Print Network [OSTI]

    Dyer, Ethan; Pufu, Silviu S

    2013-01-01T23:59:59.000Z

    We study monopole operators at the infrared fixed points of Abelian and non-Abelian gauge theories with N_f fermion flavors in three dimensions. At large N_f, independent monopole operators can be defined via the state-operator correspondence only for stable monopole backgrounds. In Abelian theories, every monopole background is stable. In the non-Abelian case, we find that many (but not all) backgrounds are stable in each topological class. We calculate the infrared scaling dimensions of the corresponding operators through next-to-leading order in 1/N_f. In the case of U(N_c) QCD with N_f fundamental fermions (and in particular in the QED case, N_c =1), we find that the monopole operators transform as non-trivial irreducible representations of the SU(N_f) flavor symmetry group.

  18. Indirect Determination of the 230Th(n,f) and 231Th(n,f) Cross Sections for Thorium-Based Nuclear Energy Systems

    E-Print Network [OSTI]

    Stroberg, S.R.

    2012-01-01T23:59:59.000Z

    n,f) Cross Sections for Thorium-Based Nuclear Energy Systemsf ) Cross Sections for Thorium-Based Nuclear Energy Systemsbenchmark- ing of advanced thorium-based nuclear reactor

  19. Microsoft Word - DOENV--FY2007.doc

    National Nuclear Security Administration (NNSA)

    CA LL NATIONAL SECURITY TECHNOLOGIES, NV AND OK 3 DP NUCLEAR FUEL SERVICES, TN NF PADUCAH GASEOUS DIFFUSION PLANT, TN PD PANTEX PLANT, TX PX PERMAFIX (M&CE), TN PF PORTSMOUTH...

  20. average mass approach: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    strange and charm quark masses performed with Nf2 twisted mass Wilson fermions. The analysis includes data at four values of the lattice spacing and pion masses as low as...

  1. Bioremediation of contaminated groundwater

    DOE Patents [OSTI]

    Hazen, T.C.; Fliermans, C.B.

    1994-01-01T23:59:59.000Z

    Disclosed is an apparatus and method for in situ remediation of contaminated subsurface soil or groundwater contaminated by chlorinated hydrocarbons. A nutrient fluid (NF) is selected to simulated the growth and reproduction of indigenous subsurface microorganisms capable of degrading the contaminants; an oxygenated fluid (OF) is selected to create an aerobic environment with anaerobic pockets. NF is injected periodically while OF is injected continuously and both are extracted so that both are drawn across the plume. NF stimulates microbial colony growth; withholding it periodically forces the larger, healthy colony of microbes to degrade the contaminants. Treatment is continued until the subsurface concentration of contaminants is acceptable. NF can be methane and OF be air, for stimulating production of methanotrophs to break down chlorohydrocarbons, especially TCE and tetrachloroethylene.

  2. Removal mechanisms of organic and inorganic solutes in raw, upland drinking water by nanofiltration: influence of solute-solute and solute-membrane interactions

    E-Print Network [OSTI]

    De Munari, Annalisa; Munari, Annalisa de

    2012-11-29T23:59:59.000Z

    Nanofiltration (NF) membranes have been applied successfully for the removal of inorganic and organic pollutants, including micropollutants, from drinking water for the past two decades. However, a complete and quantitative ...

  3. Sythhesis and Optimization of Hybrid Membrane Desalination Networks with Value Extraction

    E-Print Network [OSTI]

    AlNouss, Ahmed M

    2014-02-04T23:59:59.000Z

    into account desired process conditions and constraints that are associated with the hybrid RO-NF system. The superstructure captures all the structural and operational options that enable the extraction of a global optimal design, giving a better visualization...

  4. Effects of Dioxin on Clock, Melatonin Biosynthetic, and Redox Sensitive Genes on Chick Pinealocytes In Vitro

    E-Print Network [OSTI]

    Hunt, Jayme

    2006-07-11T23:59:59.000Z

    shown to occur via redox dependent activity of nuclear factor 1 X-type (NF1X) and cellular redox potential can regulate the circadian clock. As such we hypothesized that exposure to dioxin could affect circadian rhythmicity. We explored...

  5. Sythhesis and Optimization of Hybrid Membrane Desalination Networks with Value Extraction

    E-Print Network [OSTI]

    AlNouss, Ahmed M

    2014-02-04T23:59:59.000Z

    Membrane desalination technology has become a valuable advanced water treatment process to purify difficult water sources for potable use. Reverse Osmosis (RO) and Nanofiltration (NF) processes are commonly used desalination technologies. Studies...

  6. Genotoxicity of complex chemical mixtures

    E-Print Network [OSTI]

    Phillips, Tracie Denise

    2009-05-15T23:59:59.000Z

    aromatic hydrocarbon (PAH) fraction. The genotoxicity of these mixtures were assessed via analytical quantification, in vitro (Salmonella microsome and E. coli prophage induction) and in vivo (32P-postlabeling) bioassays. The NF was further tested...

  7. Renewable energy powered membrane technology. 2. The effect of energy fluctuations on performance of a photovoltaic hybrid membrane system

    E-Print Network [OSTI]

    Richards, B.S.; Capo, D.P.S.; Schfer, Andrea

    2008-01-01T23:59:59.000Z

    This paper reports on the performance fluctuations during the operation of a batteryless hybrid ultrafiltration nanofiltration / reverse osmosis (UF-NF/RO) membrane desalination system powered by photovoltaics treating ...

  8. Comparative Analysis of Live, Heat-inactivated, and Electron Beam Inactivated Salmonella Typhimurium Infection in Human Host Cells

    E-Print Network [OSTI]

    Corkill, Carolina

    2013-08-07T23:59:59.000Z

    Function B-actin House-keeping gene Nf-kappab Protein complex that controls the transcription of DNA; involved in cellular responses to stimuli such as stress and bacterial or viral antigens (68) Lbp Binds to bacterial lipopolysaccharide (LPS...

  9. Nitric oxide activation of Keap1/Nrf2 signaling in human colon carcinoma cells

    E-Print Network [OSTI]

    Wogan, Gerald N.

    The transcription factor NF-E2-related nuclear factor 2 (Nrf2) regulates expression of genes that protect cells from oxidative damage. Here, we characterized nitric oxide (NO)-induced Nrf2Kelch-like ECH-associated protein ...

  10. RIS-M-2408 Forsgsanlg RIS Juni 1984

    E-Print Network [OSTI]

    BARSEBACKV?RKET MED ANDRF KERNFKRAFTWRKFR NfR STORRYFR OG LAMEfiRfNSER. ' INIS Descriptors BARSEBAECK-1 REACTOR 0418-6435 INIS Descriptors: BARSEBAECK-1 REACTOR, BAERSEBAECK2 REACTOR, BELGIUM, CANADA, COMPARATIVE

  11. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050

    SciTech Connect (OSTI)

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    2015-01-01T23:59:59.000Z

    MILC asqtad QCD SU(3) gauge ensemble; series=a; a=0.11fm minus 0.0022fm ; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  12. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050b

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    MILC asqtad QCD SU(3) gauge ensemble; series=b; a=0.11fm minus 0.0022fm; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  13. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    MILC asqtad QCD SU(3) gauge ensemble; series=a; a=0.11fm minus 0.0022fm ; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  14. /NlT-2 : eAvsf's ;',,ett>v'soL .^^l ob

    E-Print Network [OSTI]

    del Barco, Enrique

    ."\\;&- o J Z, \\ LL, J^n' 1 y \\ l', = \\/A' \\anrrt hho(lo( \\ y v o ) ,nf* it.uxlx : 27T[1('t-"is) o fr,--)n P

  15. The ecology of coral-microbe interactions

    E-Print Network [OSTI]

    Marhaver, Kristen Laura

    2010-01-01T23:59:59.000Z

    algal symbioses. Molecular Ecology 18:1823-1833. Webster, N.F. Rohwer. 2008. Microbial ecology of four coral atolls inin Caribbean coral reefs. Ecology Letters 9:818-826. Porter,

  16. Drawing Graphs in Euler Diagrams Paul Mutton1, Peter Rodgers1

    E-Print Network [OSTI]

    Kent, University of

    Laboratory, University of Kent, Canterbury, Kent, CT2 7NF, UK {pjm2, P.J.Rodgers}@kent.ac.uk http://www.cs.kent.ac.uk/people/rpg/pjm

  17. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050b

    SciTech Connect (OSTI)

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    2015-01-01T23:59:59.000Z

    MILC asqtad QCD SU(3) gauge ensemble; series=b; a=0.11fm minus 0.0022fm; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  18. CUTTING EDGE Cutting Edge: Innate Immunity Conferred by B Cells Is

    E-Print Network [OSTI]

    Hedrick, Stephen M.

    CUTTING EDGE IMMUNOLOGY THE OF JOURNAL Cutting Edge: Innate Immunity Conferred by B Cells-specific Casp8-deficient mice. The activation of NF- B or IFN regulatory factor 3 was found to be unaffected

  19. An application of matroid theory to the SAT problem

    E-Print Network [OSTI]

    Kullmann, Oliver

    that a clause­set F with 1 Ÿ c(F ) Ÿ n(F ) must have a non­empty autark subset F 0 ` F , that is, F 0

  20. Design considerations for a solar-powered desalination system for remote communities in Australia

    E-Print Network [OSTI]

    Richards, B.S.; Schfer, Andrea

    2002-01-01T23:59:59.000Z

    Water in many areas of Australia is scarce and of poor quality. In some areas high levels of treatment are required either due to contamination of waters or due to high salinity. Nanofiltration (NF) and low-pressure reverse ...

  1. SHUTTLE MENU STS-135 Stow By Day

    E-Print Network [OSTI]

    *Day 1 consists of Meal C only **Day 13 consists of Meals A & B only (B) - Beverage, (FF)- Fresh Food, (I) - Irradiated, (IM) - Intermediate Moisture, (NF) - Natural Form, (R) - Rehydratable, (T

  2. ECR-Etching of Submicron and Nanometer Sized 3C-SiC(100) Mesa Lars Hiller1,a

    E-Print Network [OSTI]

    As, Donat Josef

    ). To achieve reasonable etching rates, a special gas inlet system suitable for injecting SF6 into the high, SF6, ICl, IBr, Cl2, BCl3, CH4/H2, Ar, NF3/O2, NF3/Ar, CF4 or SF6/Ar [1,2,3,4] on silicon carbide, the SF6, O2 and Ar mixing ratio, RF bias and process pressure was investigated to enhance the structure

  3. A 24GHz CMOS RF Transceiver for Car Radar Applications

    E-Print Network [OSTI]

    Nam, Sangwook

    Design In the receiving front, low noise amplifier (LNA) has a role of deciding overall receiver's noise amplifier (PA). Also, two bandgap circuits, low drop-out regulators and a temperature sensor help. Overall simulated results were S21 of 18dB, NF of 4dB and IIP3 of -6.6dBm. For mixer design, low NF

  4. Structural studies of magnesium nitride fluorides by powder neutron diffraction

    SciTech Connect (OSTI)

    Brogan, Michael A. [School of Chemistry, University of Nottingham, Nottingham NG7 2RD (United Kingdom); Hughes, Robert W. [WestCHEM, School of Chemistry, University of Glasgow, Glasgow G12 8QQ (United Kingdom); Smith, Ronald I. [ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell Oxford, Didcot OX11 0QX (United Kingdom); Gregory, Duncan H., E-mail: Duncan.Gregory@glasgow.ac.uk [WestCHEM, School of Chemistry, University of Glasgow, Glasgow G12 8QQ (United Kingdom)

    2012-01-15T23:59:59.000Z

    Samples of ternary nitride fluorides, Mg{sub 3}NF{sub 3} and Mg{sub 2}NF have been prepared by solid state reaction of Mg{sub 3}N{sub 2} and MgF{sub 2} at 1323-1423 K and investigated by powder X-ray and powder neutron diffraction techniques. Mg{sub 3}NF{sub 3} is cubic (space group: Pm3m) and has a structure related to rock-salt MgO, but with one cation site vacant. Mg{sub 2}NF is tetragonal (space group: I4{sub 1}/amd) and has an anti-LiFeO{sub 2} related structure. Both compounds are essentially ionic and form structures in which nitride and fluoride anions are crystallographically ordered. The nitride fluorides show temperature independent paramagnetic behaviour between 5 and 300 K. - Graphical abstract: Definitive structures of the ternary magnesium nitride fluorides Mg{sub 3}NF{sub 3} and the lower temperature polymorph of Mg{sub 2}NF have been determined from powder neutron diffraction data. The nitride halides are essentially ionic and exhibit weak temperature independent paramagnetic behaviour. Highlights: Black-Right-Pointing-Pointer Definitive structures of Mg{sub 3}NF{sub 3} and Mg{sub 2}NF were determined by neutron diffraction. Black-Right-Pointing-Pointer Nitride and fluoride anions are crystallographically ordered in both structures. Black-Right-Pointing-Pointer Both compounds exhibit weak, temperature independent paramagnetic behaviour. Black-Right-Pointing-Pointer The compounds are essentially ionic with ionicity increasing with F{sup -} content.

  5. Inhibition of TNF-{alpha}-mediated inflammatory responses by a benzodioxolylacetylamino-linked benzothiazole analog in human fibroblast-like synoviocytes

    SciTech Connect (OSTI)

    Lee, Young-Rae [Department of Biochemistry, Research Institute for Endocrine Sciences and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Biochemistry, Research Institute for Endocrine Sciences and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756 (Korea, Republic of); Jin, Guo Hua [College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of)] [College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of); Lee, Sang-Myeong [Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752 (Korea, Republic of)] [Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752 (Korea, Republic of); Park, Jin-Woo [Department of Biochemistry, Research Institute for Endocrine Sciences and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Biochemistry, Research Institute for Endocrine Sciences and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756 (Korea, Republic of); Ryu, Jae-Ha [College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of)] [College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of); Jeon, Raok, E-mail: rjeon@sm.ac.kr [College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of)] [College of Pharmacy, Sookmyung Women's University, 52 Hyochangwon-gil, Yongsan-gu, Seoul 140-742 (Korea, Republic of); Park, Byung-Hyun, E-mail: bhpark@jbnu.ac.kr [Department of Biochemistry, Research Institute for Endocrine Sciences and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756 (Korea, Republic of)] [Department of Biochemistry, Research Institute for Endocrine Sciences and Diabetes Research Center, Chonbuk National University Medical School, Jeonju, Jeonbuk 561-756 (Korea, Republic of)

    2011-05-20T23:59:59.000Z

    Highlights: {yields} We synthesized SPA0537, a benzothiazole analog. {yields} SPA0537 is a potent NF-{kappa}B inhibitor. {yields} SPA0537 suppresses the production of proinflammatory mediators in human rheumatoid fibroblast-like synoviocytes. {yields} SPA0537 is effective at suppressing osteoclast differentiation. -- Abstract: The pathologic processes of rheumatoid arthritis are mediated by a number of cytokines, chemokines, and matrix metalloproteinases, the expressions of which are controlled by NF-{kappa}B. This study was performed to explore the effects of a benzothiazole analog, SPA0537, on the control of the NF-{kappa}B activation pathway. We also investigated whether SPA0537 had any anti-inflammatory effects in human rheumatoid fibroblast-like synoviocytes (FLS). SPA0537 inhibited the nuclear translocation and the DNA binding of NF-{kappa}B subunits, which correlated with the inhibitory effects on IKK phosphorylation and I{kappa}B{alpha} degradation in TNF-{alpha}-stimulated rheumatoid FLS. These events further suppressed chemokine production, matrix metalloproteinase secretion, and TNF-{alpha}-induced cell proliferation. In addition, SPA0537 inhibited the osteoclast differentiation induced by macrophage colony-stimulating factor (MCSF) and receptor activator of the NF-{kappa}B ligand (RANKL) in bone marrow macrophages. These findings suggest that SPA0537 exerts anti-inflammatory effects in rheumatoid FLS through the inhibition of the NF-{kappa}B pathway. Therefore, it may have therapeutic value for the treatment of rheumatoid arthritis.

  6. Proposed natural gas protection program for Naval Oil Shale Reserves Nos. 1 and 3, Garfield County, Colorado

    SciTech Connect (OSTI)

    Not Available

    1991-08-01T23:59:59.000Z

    As a result of US Department of Energy (DOE) monitoring activities, it was determined in 1983 that the potential existed for natural gas resources underlying the Naval Oil Shales Reserves Nos. 1 and 3 (NOSrs-1 3) to be drained by privately-owned gas wells that were being drilled along the Reserves borders. In 1985, DOE initiated a limited number of projects to protect the Government's interest in the gas resources by drilling its own offset production'' wells just inside the boundaries, and by formally sharing in the production, revenues and costs of private wells that are drilled near the boundaries ( communitize'' the privately-drilled wells). The scope of these protection efforts must be expanded. DOE is therefore proposing a Natural Gas Protection Program for NOSRs-1 3 which would be implemented over a five-year period that would encompass a total of 200 wells (including the wells drilled and/or communitized since 1985). Of these, 111 would be offset wells drilled by DOE on Government land inside the NOSRs' boundaries and would be owned either entirely by the Government or communitized with adjacent private land owners or lessees. The remainder would be wells drilled by private operators in an area one half-mile wide extending around the NOSRs boundaries and communitized with the Government. 23 refs., 2 figs., 6 tabs.

  7. Naval Petroleum Reserves in California site environmental report for calendar year 1989

    SciTech Connect (OSTI)

    Not Available

    1989-01-01T23:59:59.000Z

    This summary for Naval Petroleum Reserves in California (NPRC) is divided into NPR-1 and NPR-2. Monitoring efforts at NPR-1 include handling and disposal of oilfield wastes; environmental preactivity surveys for the protection of endangered species and archaeological resources; inspections of topsoil stockpiling; monitoring of revegetated sites; surveillance of production facilities for hydrocarbons and oxides of nitrogen (NO{sub x}) emissions; monitoring of oil spill prevention and cleanup; and monitoring of wastewater injection. No major compliance issues existed for NPR-1 during 1989. Oil spills are recorded, reviewed for corrective action, and reported. Environmental preactivity surveys for proposed projects which may disturb or contaminate the land are conducted to prevent damage to the federally protected San Joaquin kit fox, blunt-nosed leopard lizard, Tipton kangaroo rat and the giant kangaroo rat. Projects are adjusted or relocated as necessary to avoid impact to dens, burrows, or flat-bottomed drainages. A major revegetation program was accomplished in 1989 for erosion control enhancement of endangered species habitat. The main compliance issue on NPR-2 was oil and produced water discharges into drainages by lessees. An additional compliance issue on NPR-2 is surface refuse from past oilfield operations. 17 refs.

  8. Endangered Species Program, Naval Petroleum Reserves in California. Annual report FY93

    SciTech Connect (OSTI)

    NONE

    1995-02-01T23:59:59.000Z

    The Naval Petroleum Reserves in California (NPRC) are operated by the US Department of Energy (DOE) and Chevron USA. Production Company (CPDN). Four federally-listed endangered animal species and one federally-threatened plant species are known to occur on NPRC: San Joaquin kit fox, blunt-nosed leopard lizard, giant kangaroo rat, Tipton kangaroo rat, and Hoover`s wooly-star. All five are protected under the Endangered Species Act of 1973, which declares that it is ``...the policy of Congress that all Federal departments and agencies shall seek to conserve endangered species and threatened species and shall utilize their authorities in furtherance of the purposes of the Act.`` DOE is also obliged to determine whether actions taken by their lessees on Naval Petroleum Reserve No. 2 will have any effects on endangered species or their habitats. The major objective of the EG&G Energy Measurements, Inc. Endangered Species Program on NPRC is to provide DOE with the scientific expertise necessary for compliance with the Endangered Species Act. The specific objective of this report is to summarize progress and results of the Endangered Species Program made during fiscal year 1993.

  9. Endangered species and cultural resources program, Naval Petroleum Reserves in California, annual report FY97

    SciTech Connect (OSTI)

    NONE

    1998-05-01T23:59:59.000Z

    The Naval Petroleum Reserves in California (NPRC) are oil fields administered by the DOE in the southern San Joaquin Valley of California. Four federally endangered animal species and one federally threatened plant species are known to occur on NPRC: San Joaquin kit fox (Vulpes macrotis mutica), blunt-nosed leopard lizard (Gambelia silus), giant kangaroo rat (Dipodomys ingens), Tipton kangaroo rat (Dipodomys nitratoides), and Hoover`s wooly-star (Eriastrum hooveri). All five are protected under the Endangered Species Act (ESA) of 1973. The DOE/NPRC is obliged to determine whether actions taken by their lessees on Naval Petroleum Reserve No. 2 (NPR-2) will have any effects on endangered species or their habitats. The primary objective of the Endangered Species and Cultural Resources Program is to provide NPRC with the scientific expertise necessary for compliance with the ESA, the National Environmental Policy Act (NEPA), and the National Historic Preservation Act (NHPA). The specific objective of this report is to summarize progress, results, and accomplishments of the program during fiscal year 1997 (FY97).

  10. Property description and fact-finding report for NPR-2, Buena Vista Hills Field, Kern County, California

    SciTech Connect (OSTI)

    NONE

    1996-06-01T23:59:59.000Z

    The US Department of Energy has asked Gustavson Associates, Inc. to serve as an Independent Petroleum Consultant under contract DE-AC01-96FE64202. This authorizes a study and recommendations regarding future development of Naval Petroleum Reserve No. 2 (NPR-2) in Kern County, California. The report that follows is the Phase 1 fact-finding and property description for that study. The United States of America owns 100 percent of the mineral rights and 96.1 percent of surface rights in 10,447 acres of the 30,182 acres contained within NPR-2. This property comprises the Buena Vista Hills Oil Field. Oil and gas companies have leased out 9,227 acres in 17 separate leases. Discovered in 1909, this field has approximately 435 active wells producing 2,819 gross barrels of oil and 8.6 million cubic feet of gas per day. Net production to the Government royalty interests include 200 barrels of oil per day and 750 thousand cubic feet of gas per day. Royalty revenues are about $1.7 million per year. Remaining recoverable reserves are approximately 407 thousand barrels of oil and 1.8 billion cubic feet of gas. Significant plugging and abandonment (P&A) and environmental liabilities are present, but these should be the responsibility of the lessees. Ultimate liability still rests with the United States and may increase as the leases are sold to smaller and smaller operators.

  11. Study of the impacts of regulations affecting the acceptance of Integrated Community Energy Systems: public utility, energy facility siting and municipal franchising regulatory programs in Ohio. Preliminary background report

    SciTech Connect (OSTI)

    Feurer, D A; Weaver, C L; Gallagher, K C; Hejna, D; Rielley, K J

    1980-01-01T23:59:59.000Z

    The Public Utilities Commission (PUCO) is a body created by the Ohio State legislature to administer the provisions of the Ohio Public Utilities Act. It is composed of three commissioners appointed by the governor with the advice and consent of the senate. Once appointed, a commissioner serves for a six-year period. The PUCO is vested with the power and jurisdiction to supervise and regulate public utilities and railroads... . The term public utility includes every corporation, company, co-partnership, person or association, their lessees, trustees, or receivers, as defined in the Ohio Code. Among the various services enumerated in the Code under the definition of public utility are an electric light company; a gas company; a pipeline company transporting gas, oil or coal; a waterworks company; a heating or cooling company. The power to regulate public utilities is shared by the PUCO and municipal governments. The municipal regulatory authority is derived from the Ohio Constitution, statutory provisions, and municipal franchising authority. Public utility regulatory statutes, energy facility siting programs, and municipal franchising authority are examined to identify how they may impact on the ability of an organization, whether or not it be a regulated utility, to construct and operate an ICES.

  12. Anomalous magnetic behavior in nanocomposite materials of reduced graphene oxide-Ni/NiFe{sub 2}O{sub 4}

    SciTech Connect (OSTI)

    Kollu, Pratap, E-mail: pk419@cam.ac.uk, E-mail: anirmalagrace@vit.ac.in, E-mail: dhirenb@iitb.ac.in [DST-INSPIRE Faculty, Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology Bombay, Mumbai 400076 (India); Prathapani, Sateesh; Varaprasadarao, Eswara K.; Mallick, Sudhanshu; Bahadur, D., E-mail: pk419@cam.ac.uk, E-mail: anirmalagrace@vit.ac.in, E-mail: dhirenb@iitb.ac.in [Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology Bombay, Mumbai 400076 (India); Santosh, Chella; Grace, Andrews Nirmala, E-mail: pk419@cam.ac.uk, E-mail: anirmalagrace@vit.ac.in, E-mail: dhirenb@iitb.ac.in [Centre for Nanotechnology Research, VIT University, Vellore 632014 (India)

    2014-08-04T23:59:59.000Z

    Magnetic Reduced Graphene Oxide-Nickel/NiFe{sub 2}O{sub 4} (RGO-Ni/NF) nanocomposite has been synthesized by one pot solvothermal method. Respective phase formations and their purities in the composite are confirmed by High Resolution Transmission Electron Microscope and X Ray Diffraction, respectively. For the RGO-Ni/NF composite material finite-size effects lead to the anomalous magnetic behavior, which is corroborated in temperature and field dependent magnetization curves. Here, we are reporting the behavior of higher magnetization values for Zero Field Cooled condition to that of Field Cooled for the RGO-Ni/NF nanocomposite. Also, the observed negative and positive moments in Hysteresis loops at relatively smaller applied fields (100?Oe and 200?Oe) are explained on the basis of surface spin disorder.

  13. Delineating role of ubiquitination on nuclear factor-kappa B pathway by a computational modeling approach

    SciTech Connect (OSTI)

    Lee, Jungsul; Choi, Kyungsun [Cell Signaling and BioImaging Laboratory, Department of Bio and Brain Engineering, KAIST, Daejeon 305-701 (Korea, Republic of)] [Cell Signaling and BioImaging Laboratory, Department of Bio and Brain Engineering, KAIST, Daejeon 305-701 (Korea, Republic of); Choi, Chulhee, E-mail: cchoi@kaist.ac.kr [Cell Signaling and BioImaging Laboratory, Department of Bio and Brain Engineering, KAIST, Daejeon 305-701 (Korea, Republic of) [Cell Signaling and BioImaging Laboratory, Department of Bio and Brain Engineering, KAIST, Daejeon 305-701 (Korea, Republic of); Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701 (Korea, Republic of); KI for Bio Century, KAIST, Daejeon 305-701 (Korea, Republic of)

    2010-01-01T23:59:59.000Z

    Mutant ubiquitin found in neurodegenerative diseases has been thought to hamper activation of transcription factor nuclear factor-kappa B (NF-{kappa}B) by inhibiting ubiquitin-proteasome system (UPS). It has been reported that ubiquitin also is involved in signal transduction in an UPS-independent manner. We used a modeling and simulation approach to delineate the roles of ubiquitin on NF-{kappa}B activation. Inhibition of proteasome complex increased maximal activation of IKK mainly by decreasing the UPS efficiency. On the contrary, mutant ubiquitin decreased maximal activity of IKK. Computational modeling showed that the inhibition effect of mutant ubiquitin is mainly attributed to decreased activity of UPS-independent function of ubiquitin. Collectively, our results suggest that mutant ubiquitin affects NF-{kappa}B activation in an UPS-independent manner.

  14. Quantum and stringy corrections to the equation of state of holographic QCD matter and the nature of the chiral transition

    E-Print Network [OSTI]

    T. Alho; M. Jarvinen; K. Kajantie; E. Kiritsis; K. Tuominen

    2015-03-26T23:59:59.000Z

    We consider the finite temperature phase diagram of holographic QCD in the Veneziano limit (Nc large, Nf large with xf=Nf/Nc fixed) and calculate one string-loop corrections to the free energy in certain approximations. Such corrections, especially due to the pion modes are unsuppressed in the Veneziano limit. We find that under some extra assumptions the first order transition following from classical gravity solutions can become second order. If stringy asymptotics are of a special form and there are residual interactions it may even become of third order. Operationally these computations imply modelling the low temperature chiral symmetry breaking phase with a hadron gas containing Nf^2 massless Goldstone bosons and an exponential spectrum of massive hadrons. A third order transition is possible only if repulsive hadron interactions via the excluded volume effect are included.

  15. Marvell NanoLab Member login Lab Manual Contents MercuryWeb Berkeley Microlab Chapter 7.2

    E-Print Network [OSTI]

    Healy, Kevin Edward

    Sf6. b) 3.Nf3 Vg4 4.Le2 Sc6 5.d4 0-0-0. 3.Nc3 Wa5 (D) XABCDEFGHY 8rsnl+kvlntr( 7zppzp-zppzpp' 6-+-+-+-+& 5wq-+-+-+-% 4-+-+-+-+$ 3+-sN-+-+-# 2PzPPzP-zPPzP" 1tR-vLQmKLsNR! xabcdefghy a) 4.Nf3 Sf6 5.Le2 Black to prevent f5 ...Sf6 5.d3 threatening Ng1-e2-g3 and Pf2-f4-f5 pressure on the kingside Sc6 6.Ld2 Wf5. 4.d4 Sf

  16. strategy.doc 17/11/2005 1/28 strategy.doc 17/11/2005 2/28

    E-Print Network [OSTI]

    Rostock, Universitt

    Sf6. b) 3.Nf3 Vg4 4.Le2 Sc6 5.d4 0-0-0. 3.Nc3 Wa5 (D) XABCDEFGHY 8rsnl+kvlntr( 7zppzp-zppzpp' 6-+-+-+-+& 5wq-+-+-+-% 4-+-+-+-+$ 3+-sN-+-+-# 2PzPPzP-zPPzP" 1tR-vLQmKLsNR! xabcdefghy a) 4.Nf3 Sf6 5.Le2 Black to prevent f5 ...Sf6 5.d3 threatening Ng1-e2-g3 and Pf2-f4-f5 pressure on the kingside Sc6 6.Ld2 Wf5. 4.d4 Sf

  17. IEA W-GIFT-6 SMALL SPECIMEN TEST TECHNIQUE

    E-Print Network [OSTI]

    McDonald, Kirk

    IEA W-GIFT-6 SMALL SPECIMEN TEST TECHNIQUE UNDER IFMIF/EVEDA PROJECT OF BA FOR FATIGUE AND FRACTURE,t[%] Number of cycles to failure, Nf F82H-IEA HG-1.25 HG-1.25 (Hirose, Kim) RB-1 RB-7 RB-4, -7 RB-7, -10 of cycles to failure, Nf JLF-1 HG-1.25 HG-1.25 HG-6 (Hirose) RB-1 RB-8 (Nishimura&Li) F82H-IEA (Grain size

  18. Corso di Controlli Automatici Prof. Stefano Di Gennaro

    E-Print Network [OSTI]

    Pola, Giordano

    (s)P(s). Il luogo delle radici descritto dall'equazione DW (s) = K0 (s 1) + (s + 1) (s 5) = s2 + (K0 4) s K0 s 1 (s + 1) (s 5) = K0 s 1 (s 5) (s 6) La funzione di trasferimento del sistema ad anello chiuso W(s) = NW (s) DW (s) = NF0 (s) NF0 (s) + DF0 (s) = K0 (s 1) K0 (s 1) + (s 5) (s 6) Il luogo delle radici

  19. Chemopreventive Potential of Sorghum with Different Phenolic Profiles

    E-Print Network [OSTI]

    Yang, Liyi

    2010-07-14T23:59:59.000Z

    potential damage from free radicals to cells (45). The cancer initiation blockers can also stimulate molecular pathways, such as Nrf2 (nuclear factor-erythroid 2p45 (NF- E2)-related factor 2) to activate the production of protective phase II enzymes... elements (AREs). Basic leucine zipper (bZIP) transcription factors ? including NRF (NF-E2 related factor)? bind to these ARE sequences and modulate expression of stress-response genes. NRF2-KEAP1 (Kelch-like ECH-associated protein 1, a negative regulator...

  20. Report on thermal aging effects on tensile properties of ferritic-martensitic steels.

    SciTech Connect (OSTI)

    Li, M.; Soppet, W.K.; Rink, D.L.; Listwan, J.T.; Natesan, K. (Nuclear Engineering Division)

    2012-05-10T23:59:59.000Z

    This report provides an update on the evaluation of thermal-aging induced degradation of tensile properties of advanced ferritic-martensitic steels. The report is the first deliverable (level 3) in FY11 (M3A11AN04030103), under the Work Package A-11AN040301, 'Advanced Alloy Testing' performed by Argonne National Laboratory, as part of Advanced Structural Materials Program for the Advanced Reactor Concepts. This work package supports the advanced structural materials development by providing tensile data on aged alloys and a mechanistic model, validated by experiments, with a predictive capability on long-term performance. The scope of work is to evaluate the effect of thermal aging on the tensile properties of advanced alloys such as ferritic-martensitic steels, mod.9Cr-1Mo, NF616, and advanced austenitic stainless steel, HT-UPS. The aging experiments have been conducted over a temperature of 550-750 C for various time periods to simulate the microstructural changes in the alloys as a function of time at temperature. In addition, a mechanistic model based on thermodynamics and kinetics has been used to address the changes in microstructure of the alloys as a function of time and temperature, which is developed in the companion work package at ANL. The focus of this project is advanced alloy testing and understanding the effects of long-term thermal aging on the tensile properties. Advanced materials examined in this project include ferritic-martensitic steels mod.9Cr-1Mo and NF616, and austenitic steel, HT-UPS. The report summarizes the tensile testing results of thermally-aged mod.9Cr-1Mo, NF616 H1 and NF616 H2 ferritic-martensitic steels. NF616 H1 and NF616 H2 experienced different thermal-mechanical treatments before thermal aging experiments. NF616 H1 was normalized and tempered, and NF616 H2 was normalized and tempered and cold-rolled. By examining these two heats, we evaluated the effects of thermal-mechanical treatments on material microstructures and associated mechanical properties during long-term aging at elevated temperatures. Thermal aging experiments at different temperatures and periods of time have been completed: 550 C for up to 5000 h, 600 C for up to 7500 h, and 650 C for more than 10,000 h. Tensile properties were measured on thermally aged specimens and aging effect on tensile behavior was assessed. Effects of thermal aging on deformation and failure mechanisms were investigated by using in-situ straining technique with simultaneous synchrotron XRD measurements.

  1. K semileptonic form factor with HISQ fermions at the physical point

    E-Print Network [OSTI]

    E. Gmiz; A. Bazavov; C. Bernard; C. Bouchard; C. DeTar; D. Du; A. X. El-Khadra; J. Foley; E. D. Freeland; Steven Gottlieb; U. M. Heller; J. Kim; A. S. Kronfeld; J. Laiho; L. Levkova; P. B. Mackenzie; E. T. Neil; M. B. Oktay; Si-Wei Qiu; J. N. Simone; R. Sugar; D. Toussaint; R. S. Van de Water; Ran Zhou

    2014-08-17T23:59:59.000Z

    We present results for the form factor $f_+^{K \\pi}(0)$, needed to extract the CKM matrix element $|V_{us}|$ from experimental data on semileptonic $K$ decays, on the HISQ $N_f=2+1+1$ MILC configurations. The HISQ action is also used for the valence sector. The data set used for our final result includes three different values of the lattice spacing and data at the physical light quark masses. We discuss the error budget and how this calculation improves on our previous determination of $f_+^{K \\pi}(0)$ on the asqtad $N_f=2+1$ MILC configurations.

  2. Holographic Viscosity of Fundamental Matter

    E-Print Network [OSTI]

    David Mateos; Robert C. Myers; Rowan M. Thomson

    2006-10-16T23:59:59.000Z

    A holographic dual of a finite-temperature SU(N_c) gauge theory with a small number of flavours N_f viscosity to entropy ratio in these theories saturates the conjectured universal bound eta/s >= 1/4\\pi. The contribution of the fundamental matter eta_fund is therefore enhanced at strong 't Hooft coupling lambda; for example, eta_fund ~ lambda N_c N_f T^3 in four dimensions. Other transport coefficients are analogously enhanced. These results hold with or without a baryon number chemical potential.

  3. Running of the SF-coupling with four massless flavours

    E-Print Network [OSTI]

    Rainer Sommer; Fatih Tekin; Ulli Wolff

    2010-11-10T23:59:59.000Z

    We discuss the status of different determinations of alpha_s, motivating a precise and reliable computation from lattice QCD. In order to suppress perturbative errors, the non-perturbative computation has to reach high energy scales mu. Such results already exist in the SF-scheme for N_f=0,2 and N_f=3. We recently added the running with four massless flavours in a range of alpha from about 0.07 to 0.3 . It is based on our recent determination of the Sheikholeslami Wohlert coefficient in the four-flavour theory.

  4. Nepali Aawaz Volume 1, Issue 9, 24 January - 7 February 2006

    E-Print Network [OSTI]

    Shrestha, Kashish Das

    |:t'tLn] tkfO{x?nfO{ ;Gt'i6 kfg'{ d]/f] p2]Zo xf] eGb} lyP . >j0f s'ln{Pgg, dlng : j/ / uDeL/} d'b|fdf Oltxf;nfO{ lwsfb}{ s'ln{Psf lj;] gurL{sf] jofg pgn] jfrg u/]kl5 >f]tfsf] tfnL u8u8fx6 ;'g]/ wGojfb lbb} d':s'/fP . slj s[i0f e'if0f jnn] hgtfsf] cfjfh...

  5. N-3 FATTY ACIDS AND JUVENILE TRAUMATIC BRAIN INJURY: EFFECTS OF DIETARY N-3 FATTY ACID CONTENT, N-3 FATTY ACID STATUS, AND ORALLY DOSED FISH OIL ON SENSORIMOTOR AND BIOCHEMICAL OUTCOMES

    E-Print Network [OSTI]

    Russell, Kristin L.

    2013-08-31T23:59:59.000Z

    MMP Matrix metalloproteinase MUFA Monounsaturated fatty acid NF-?B Nuclear factor kappa B NMDA N-methyl-D-aspartic acid NPD1 Neuroprotectin D1 xii Abbreviation Full Name PBF Phosphate buffered formalin PBS Phosphate buffered...). Transcription of a pro-MMP-2 and pro-MMP-9 is regulated by nuclear factor kappa B (NF-?B) (Gottschall and Deb, 1996). The pro- forms are cleaved to active forms by each other and other MMPs when necessary (Nagase et al., 2006). MMPs are endogenously...

  6. Use of nanofiltration to reduce cooling tower water usage.

    SciTech Connect (OSTI)

    Sanchez, Andres L.; Everett, Randy L.; Jensen, Richard Pearson; Cappelle, Malynda A.; Altman, Susan Jeanne

    2010-09-01T23:59:59.000Z

    Nanofiltration (NF) can effectively treat cooling-tower water to reduce water consumption and maximize water usage efficiency of thermoelectric power plants. A pilot is being run to verify theoretical calculations. A side stream of water from a 900 gpm cooling tower is being treated by NF with the permeate returning to the cooling tower and the concentrate being discharged. The membrane efficiency is as high as over 50%. Salt rejection ranges from 77-97% with higher rejection for divalent ions. The pilot has demonstrated a reduction of makeup water of almost 20% and a reduction of discharge of over 50%.

  7. Use of nanofiltration to reduce cooling tower water consumption.

    SciTech Connect (OSTI)

    Altman, Susan Jeanne; Ciferno, Jared

    2010-10-01T23:59:59.000Z

    Nanofiltration (NF) can effectively treat cooling-tower water to reduce water consumption and maximize water usage efficiency of thermoelectric power plants. A pilot is being run to verify theoretical calculations. A side stream of water from a 900 gpm cooling tower is being treated by NF with the permeate returning to the cooling tower and the concentrate being discharged. The membrane efficiency is as high as over 50%. Salt rejection ranges from 77-97% with higher rejection for divalent ions. The pilot has demonstrated a reduction of makeup water of almost 20% and a reduction of discharge of over 50%.

  8. Estimating Translational and Orientational Entropies Using the k-Nearest Neighbours Algorithm

    E-Print Network [OSTI]

    Huggins, David J.

    2014-05-19T23:59:59.000Z

    Estimating Translational and Orientational Entropies Using the k?Nearest Neighbors Algorithm David J. Huggins* Theory of Condensed Matter Group, Cavendish Laboratory, University of Cambridge, 19 J J Thomson Avenue, Cambridge CB3 0HE, United Kingdom... = + = = ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? S nR nF d nF V 1 ln 4 3 i F j n i sw,trans 1 1 trans 3 (4) R is the gas constant, F is the number of frames sampled, Vi is the volume of the system in frame i, and ? is Eulers constant, which corrects for the asymptotic bias. In this case the nearest...

  9. Ferroelectric and dielectric properties of ferrite-ferroelectric ceramic composites

    SciTech Connect (OSTI)

    Elena Ciomaga, Cristina; Maria Neagu, Alexandra; Valentin Pop, Mihai; Mitoseriu, Liliana [Faculty of Physics, 'Al. I. Cuza' University of Iasi 700506, Iasi (Romania); Airimioaei, Mirela [Faculty of Chemistry, 'Al. I. Cuza' University of Iasi 700506, Iasi, Romania and Dept. Chemistry and Process Engineering, University of Genoa, P-le Kennedy no. 1, I-16129, Genoa (Italy); Tascu, Sorin [RAMTECH Faculty of Physics, 'Al. I. Cuza' University of Iasi 700506 (Romania); Schileo, Giorgio [Christian Doppler Laboratory for Advanced Ferroic Oxides, Sheffield Hallam University, Howard Street, Sheffield S1 1WB (United Kingdom); Galassi, Carmen [CNR-ISTEC, Via Granarolo no. 64, I-48018 Faenza (Italy)

    2013-02-21T23:59:59.000Z

    Particulate composites of ferrite and ferroelectric phases with xNiFe{sub 2}O{sub 4} (NF) and (1 - x)Pb{sub 0.988}(Zr{sub 0.52}Ti{sub 0.48}){sub 0.976}Nb{sub 0.024}O{sub 3} (where x = 2, 10, 20, 30, 50, 70, and 100 wt. %) were prepared in situ by sol-gel method. The presence of a diphase composition was confirmed by X-ray diffraction while the microstructure of the composites was studied by scanning electron microscopy revealing a good mixing of the two phases and a good densification of the bulk ceramics. The dielectric permittivity shows usual dielectric dispersion behavior with increasing frequency due to Maxwell-Wagner interfacial polarization. AC conductivity measurements made in frequency range 1 Hz-1 MHz suggest that the conduction process is due to mixed polaron hopping. The effect of NF phase concentration on the P-E and M-H hysteresis behavior and dielectric properties of the composites was investigated. At low NF concentration a sharp ferro-paraelectric transition peak can be observed at around 360 Degree-Sign C while for higher NF concentrations a trend to a diffuse phase transition occurs. All the composite samples exhibit typical ferromagnetic hysteresis loops, indicating the presence of ordered magnetic structure.

  10. Infrared finite coupling in Sudakov resummation

    E-Print Network [OSTI]

    Georges Grunberg

    2006-02-06T23:59:59.000Z

    New arguments are presented to emphasize the interest of the infrared finite coupling approach to power corrections in the context of Sudakov resummation. The more regular infrared behavior of some peculiar combinations of Sudakov anomalous dimensions, free of Landau singularities at large Nf, is pointed out. A general conflict between the infrared finite coupling and infrared renormalon approaches to power corrections is explained, and a possible resolution is proposed, which makes use of the arbitrariness of the choice of exponentiated constant terms. A simple ansatz for a 'universal' non-perturbative Sudakov effective coupling at large Nf follows naturally from these considerations. In this last version, a new result is presented: the striking emergence of an infrared finite perturbative effective coupling in the Drell-Yan process at large Nf (at odds with the infrared renormalon argument) within the framework of Sudakov resummation for eikonal cross sections of Laenen, Sterman and Vogelsang. Some suggestions for phenomenology at finite Nf, alternative to the shape function approach, are given.

  11. Exam revision Database Systems Lecture 18

    E-Print Network [OSTI]

    Alechina, Natasha

    ,B) and (A,C), fd B C. #12;3 Exam revision Example (coursework 5) · Relation Listing is not in 2NF because1 Exam revision Database Systems Lecture 18 Natasha Alechina Exam revision In This Lecture · Exam format · Main topics · How to revise Exam revision Exam format · Answer three questions out of five

  12. High-numerical aperture holographic data storage Floris M. H. Crompvoets, Frank Schuurmans, Marcello Balistreri, Teus Tukker, Gert 't Hoofi,

    E-Print Network [OSTI]

    Stallinga, Sjoerd

    ,4 this technology has gained a lot of attention by the optical storage industry. The promise of HDS-layer Blu-ray Disc (ML-BD)5 and 'Near Field' (NF) recording6. The applications aimed at by the next generation optical storage are for instance professional and personal archiving. A comparison of the overall

  13. Neutrino Factory Mercury Flow Loop

    E-Print Network [OSTI]

    McDonald, Kirk

    Could require double containment of mercury Chase will certainly have a drain back into hot cell Decay 2010 #12;Hg Flow Overflow Minimize pressure drops through piping Overflow Mercury Drain drops Gravity Drain Beam Dumptransitioning to 1 cm nozzle Actual NF Hg inventory may reach SNS Gravity Drain

  14. (BBA) is competent for investigations on incidents of poisoning honey bees due to the use of pesti-

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    (BBA) is competent for investigations on incidents of poisoning honey bees due to the use of pesti in the case of honey-bee poisoning by pesticides' [Amtl Pfl Best (Berlin-Dahlem), NF 36/5, 179-182]. An overview of the poisoning incidents shows a clear decline in the last years. The most impor- tant

  15. COMBUSTION DRIVEN ATOMIC FLUORINE GENERATORS FOR DF CHEMICAL LASERS Abstract -Conventional high pressure corabustors delivering atomic fluorine for DF chemical

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    compositions assuming frozen isentropic expansion and with or without heat losses in the combustor. The values production at good overall laser power. 1. INTRODUCTION Available references describing combustors for also reactants. Solid propellant com- high production of F atom by thermal equilibrium positions such as NF

  16. Atmos. Chem. Phys., 12, 36533658, 2012 www.atmos-chem-phys.net/12/3653/2012/

    E-Print Network [OSTI]

    Boyer, Edmond

    phased out. An earlier observation of closely correlated atmospheric abun- dances of SF5CF3 and SF6uhle et al., 2010), c-C4F8 (Oram et al., 2012), NF3 (Weiss et al., 2008) and SF6 (Levin et al., 2010 of CF+ 3 and SF+ 5 ions during measurements of chlorofluorocarbons and SF6 in air samples from

  17. 309.ps.gz - Optimization Online

    E-Print Network [OSTI]

    Keywords: Large scale optimization; Nonlinear programming; Electricity gen- .... voirs and Nu thermal units that can work with Nf di erent fuel types (e.g., gas, nuclear, coal, etc.). 2 ...... where CONT is some limit to the emission of pollutants, i

  18. Effects of atomic hydrogen and deuterium exposure on high polarization GaAs photocathodes M. Baylac,* P. Adderley, J. Brittian, J. Clark, T. Day, J. Grames, J. Hansknecht, M. Poelker, M. Stutzman, and A. T. Wu

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    chambers. The photocathode is formed when cesium (Cs) and nitrogen trifluoride (NF3) gas are applied cleaning remains an effective technique to remove surface contamination from bulk GaAs. A note- worthy. This statement stems from the realization, made over the course of years, that contamination is introduced onto

  19. On Dynamic Vehicle Routing with Time Constraints Shaunak D. Bopardikar Stephen L. Smith Francesco Bullo

    E-Print Network [OSTI]

    Bullo, Francesco

    ] and in [12]. Both of these two references deal with a problem which is essentially a dual of the one we work supported in part by NSF Award CPS- 1035917 and ARO Award W911NF-11-1-0092. S. D. Bopardikar

  20. Kalman Filters to Reduce Noise Effects during External

    E-Print Network [OSTI]

    Mauel, Michael E.

    Kalman Filters to Reduce Noise Effects during External Kink Control M. E. Mauel, J. Farrington, J for the implementation of a Kalman filter that tracks kink mode dynamics as recently described (M. E. Mauel, et al., NF Simple digital filter Kalman filter Key Results Feedback control of HBT-EP and DIII-D requires

  1. Nutrition, digestive system and digestion specificity in phytophagous bats

    E-Print Network [OSTI]

    Boyer, Edmond

    Nutrition, digestive system and digestion specificity in phytophagous bats NF Zhukova Schmalhausen arisen as to how the feeding and digestion specificity is revealed in phytophagous bats, and how the entire order specificity affects these processes. To tackle these issues, the digestive system

  2. OSP WEEKLY FUNDING BULLETIN Volume 5, Issue 33 August 15, 2011

    E-Print Network [OSTI]

    Alabama in Huntsville, University of

    (MRI) (modification) Solicitation No. W911NF-11-R-0011CFDA No. Due Date: White Papers: 14 September Solicitation No. DARPA-BAA-11-65 CFDA No. 12.910 Due Date: 9 August 2012 http Center CFDA Number 84.326J Solicitation No. ED-GRANTS-080911-002 CFDA No. 84.326 Due Date: 8 September

  3. OSP WEEKLY FUNDING BULLETIN Volume 5, Issue 44 October 31, 2011

    E-Print Network [OSTI]

    Alabama in Huntsville, University of

    -BAA-11-60 CFDA No. 12.910 Due Date: 2 December 2011 http://www07.grants and Development Program (modification) Solicitation No. W911NF-12-R-0001 CFDA No. 12.630 Due Date: White Papers-AFOSR-2012-02 CFDA No. 12.800 Due Date: 23 November 2012 http://www07.grants

  4. Adsorption of small organic solutes from beet distillery condensates on reverse-osmosis membranes: consequences on the process performances

    E-Print Network [OSTI]

    Boyer, Edmond

    at the fermentation stage. However, transfer mechanisms through dense NF and RO membranes are still not well of the sorption isotherms of five target inhibitory compounds (acetic and butanoic acids, furfural, 2-phenethyl alone as well as mixed in order to evaluate the competition effects. Results reveal that acetic acid

  5. System level design of power distribution network for mobile computing platforms

    E-Print Network [OSTI]

    Shayan Arani, Amirali

    2011-01-01T23:59:59.000Z

    for Low frequency voltage droop impact on F max for multiplehundred of nF . Figure 2.2 VDD |z| 1st droop Motherboard2 nd droop 3 rd droop 2 nd droop 3 rd droop Log(freq) ~1ns ~

  6. 2012NatureAmerica,Inc.Allrightsreserved. Nature GeNetics ADVANCE ONLINE PUBLICATION

    E-Print Network [OSTI]

    Nyholt, Dale R.

    GWASdatasetidentifiedexoDNase,NF-kBsignalingandmitochondrialdysfunctionasbiologicalprocessesrelatedto timingofmenopause. RESULTS In our discovery stage of 38,968 women with natural menopause aged 4060 (Supplementary in effect size for the 20 SNPs in retrospectively and prospectively col- lected menopause age, and found at natural menopause in women from the Women's Genome Health Study (WGHS). For early meno- pause, we compared

  7. Quercetin and Chlorogenic Acid Mitigate DSS-Induced Changes in Expression of Select Pro-Inflammatory Cytokines and Short Chain Fatty Acid Transporter Genes

    E-Print Network [OSTI]

    Piefer, Leigh

    2012-10-19T23:59:59.000Z

    oxide synthase LPS lipopolysaccharide LS least squares MCT-1 monocarboxylate transporter-1 MyD88 myeloid differentiation factor-88 NF-?B nuclear transcription factor kappa B PBS phosphate buffered solution PFA paraformaldehyde RT-PCR reverse... animals (acetic acid, pMCT-1 and SLC5A8 mRNA levels show that DSS significantly...

  8. Stereoselective Total Synthesis of (+)-Norrisolide Thomas P. Brady, Sun Hee Kim, Ke Wen, and Emmanuel A. Theodorakis*

    E-Print Network [OSTI]

    Theodorakis, Emmanuel

    ) at highest commercial quality and used without further purification except where noted. Air- and moisture2Cl2) and benzene (PhH) were purified by passage through a bed of activated alumina.1 NF-254) using UV light as the visualizing agent and 10% ethanolic phosphomolybdic acid (PMA) or p

  9. ALGEBRA LINEAL I 1er Curso de CC. Fisicas. Examen Final. 4 de Febrero de 2005.

    E-Print Network [OSTI]

    Guijarro, Luis

    ALGEBRA LINEAL I 1er Curso de CC. Fisicas. Examen Final. 4 de Febrero de 2005. Apellidos: Nombre aplicacion lineal f : R3 R3 tal que Kerf = Nf x -y = 0 y -z = 0 Imf x + 2y + 3z = 0. 4. Sean L, U y B

  10. Dynamics of the Dorsal morphogen gradient Jitendra S. Kanodiaa

    E-Print Network [OSTI]

    Shvartsman, Stanislav "Stas"

    Dynamics of the Dorsal morphogen gradient Jitendra S. Kanodiaa , Richa Rikhyb , Yoosik Kima Road, Princeton, NJ 08544; bCell Biology and Metabolism Branch, NIH, Building 32, 18 Library Drive localization gradient of Dorsal (Dl), a protein related to the mammalian NF- B transcription factors. Current

  11. Interchange turbulence simulations for JET relevant parameters

    E-Print Network [OSTI]

    , Culham Science center, Abingdon UK W. Fundamenski et al, subm. NF (2006) IEA Large Tokamak IA Workshop on Edge Transport in Fusion Plasmas #12;IEA Large Tokamak IA Workshop on Edge Transport in Fusion Plasmas from first principal #12;IEA Large Tokamak IA Workshop on Edge Transport in Fusion Plasmas ESEL code

  12. Non-perturbative beta function in three-dimensional electrodynamics

    E-Print Network [OSTI]

    Ohad Raviv; Yigal Shamir; Benjamin Svetitsky

    2014-05-27T23:59:59.000Z

    We apply the Schrodinger functional method to the Abelian gauge theory in three dimensions with Nf=2 four-component fermions. We find that the calculated beta function does not cross zero in the range of coupling we study. This implies that the theory exhibits confinement and mass generation, rather than a conformal infared regime.

  13. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31T23:59:59.000Z

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  14. INSTITUTE OF PHYSICS PUBLISHING and INTERNATIONAL ATOMIC ENERGY AGENCY NUCLEAR FUSION Nucl. Fusion 43 (2003) 16931709 PII: S0029-5515(03)67272-8

    E-Print Network [OSTI]

    Ghoniem, Nasr M.

    2003-01-01T23:59:59.000Z

    INSTITUTE OF PHYSICS PUBLISHING and INTERNATIONAL ATOMIC ENERGY AGENCY NUCLEAR FUSION Nucl. Fusion 43 (2003) 16931709 PII: S0029-5515(03)67272-8 Fusion energy with lasers, direct drive targets.iop.org/NF/43/1693 Abstract A coordinated, focused effort is underway to develop Laser Inertial Fusion Energy

  15. IOP PUBLISHING and INTERNATIONAL ATOMIC ENERGY AGENCY NUCLEAR FUSION Nucl. Fusion 50 (2010) 014004 (14pp) doi:10.1088/0029-5515/50/1/014004

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    IOP PUBLISHING and INTERNATIONAL ATOMIC ENERGY AGENCY NUCLEAR FUSION Nucl. Fusion 50 (2010) 014004.iop.org/NF/50/014004 Abstract Fusion energy research began in the early 1950s as scientists worked to harness at demonstrating fusion energy producing plasmas. PACS numbers: 52.55.-s, 52.57.-z, 28.52.-s, 89.30.Jj (Some

  16. -Page 1 of 5 -Minutes of Meeting

    E-Print Network [OSTI]

    Burton, Geoffrey R.

    of Electronic & Electrical Engineering Prof JB Chaudhuri JBC Associate Dean, Research Dr Jos Darling JD Dept of Electronic & Electrical Engineering Mr Nello Formisano NF PGR Student Rep, Dept of Elec Eng Mr Martin Mitchell NJM Dept of Electronic & Electrical Eng Dr Kevin Paine KP Dept of Architecture & Civil Engineering

  17. -Page 1 of 4 -Minutes of Meeting

    E-Print Network [OSTI]

    Burton, Geoffrey R.

    of Chemical Engineering Dr Ivan Astin IA Dept of Electronic & Electrical Engineering Dr Steve Cayzer SC2 Dept Dr Adrian Evans AE Dept of Electronic & Electrical Engineering Mr Nello Formisano NF PGR Student RepD Dept of Electronic & Electrical Engineering) noted that two colleagues had been promoted Dr Martin

  18. Molecular Cell, Volume 37 Supplemental Information

    E-Print Network [OSTI]

    Bulyk, Martha L.

    Inputs Luca Giorgetti, Trevor Siggers, Guido Tiana, Greta Caprara, Samuele Notarbartolo, Teresa Corona-kB nuclear concentration. (B) Mean Pol II occupancy on the core promoter as a function of NF-kB nuclear 1, while site 0 represents the core promoter where Pol II exchanges), its occupancy is given by 1

  19. Effects of supplementary feed enzymes on the laying performance and fecal moisture of a commercial incrossbred egg stock

    E-Print Network [OSTI]

    Ibarbia, Ramon Amador

    1964-01-01T23:59:59.000Z

    offeots of eater treatment of barley and fungal snmpne suyylsmsntation on Che metabolisahle . nf- %he raMonx-' ai-ref&i+ted bf Cha-:perfc~- of -gi ouing- ohkobd:: ?. The authors reported inoreassi-in the msCaboliga&e energy-as a rema& of the addition...

  20. Literature review Precipitation in creep resistant austenitic

    E-Print Network [OSTI]

    Cambridge, University of

    and titanium additions. Conventional alloys such as 18 8 and 16 10 are included together with the new NF709 of Z phase in nitrogen bearing steels is a further complicating factor, and it is concluded that its and titanium, both of which getter carbon. Other compounds reviewed include x and G phases, which form at high

  1. Engineering Radio resource management for OFDMA systems under practical

    E-Print Network [OSTI]

    Martin, Ralph R.

    in the construction industry / Bin Chen. Ph.D. 2009 NF nanofiltration membrane characterization / Ahmed Saleh Al verification / Neil Pearson. M.Phil. 2008 #12; Design and optimisation of joints in a carbon fibre bicycle reservoir 'A' / Hussain Al-Hassani. M.Res. 2008 Chemical Engineering Tensile properties of polymeric

  2. Sum Rules and Cutoff Effects in Wilson Lattice QCD

    E-Print Network [OSTI]

    Harvey B. Meyer

    2006-09-06T23:59:59.000Z

    We use the transfer matrix formalism to derive non-perturbative sum rules in Wilson's lattice QCD with N_f flavours of quarks. The discretization errors on these identities are treated in detail. As an application, it is shown how the sum rules can be exploited to give improved estimates of the continuum spectrum and static potential.

  3. Asymptotic scaling corrections in QCD with Wilson fermions from the 3-loop average plaquette

    E-Print Network [OSTI]

    B. Alles; A. Feo; H. Panagopoulos

    1998-01-23T23:59:59.000Z

    We calculate the 3-loop perturbative expansion of the average plaquette in lattice QCD with N_f massive Wilson fermions and gauge group SU(N). The corrections to asymptotic scaling in the corresponding energy scheme are also evaluated. We have also improved the accuracy of the already known pure gluonic results at 2 and 3 loops.

  4. IEEE TRANSACTIONS ON MICROWAVE THEORY AND TECHNIQUES, VOL. 60, NO. 5, MAY 2012 1331 A CMOS Distributed Amplifier With Distributed

    E-Print Network [OSTI]

    Heydari, Payam

    to improve BW. A distributed low- noise amplifier (DLNA) is designed for minimum noise figure (NF) for ultra methods have been proposed, as well [16][20]. In [16], a low-frequency amplifier and a high Distributed Amplifier With Distributed Active Input Balun Using GBW and Linearity Enhancing Techniques Amin

  5. A Systematic Approach to CMOS Low Noise Amplifier Design for Ultrawideband Applications

    E-Print Network [OSTI]

    Ha, Dong S.

    A Systematic Approach to CMOS Low Noise Amplifier Design for Ultrawideband Applications Hyung method to design CMOS low noise amplifier (LNA) for ultrawideband (UWB) applications. The proposed method matching. The synthesized LNA achieves up to 14dB power gain with a low noise figure (NF) of 2d

  6. Reflective Cracking Study: First-level Report on Laboratory Shear Testing

    E-Print Network [OSTI]

    Tsai, Bor-Wen; Jones, David; Harvey, John T; Monismith, Carl L.; Guada, I.; Signore, J,

    2008-01-01T23:59:59.000Z

    2 and ? 2 ln? 3 and ? 3 lnG lnkcy5 lnn 1 lnn 2 lnNf lnpct559 Figure 4.28: Residual plots of LnG. (Pooled ShearResilient Shear Modulus, G* (lng) The Resilient Shear

  7. BREAST CANCER GROUP WOMEN'S HEALTH INTERDISCIPLINARY

    E-Print Network [OSTI]

    Spence, Harlan Ernest

    BREAST CANCER GROUP May 2009 WOMEN'S HEALTH INTERDISCIPLINARY RESEARCH CENTER [WHIRC] #12;2 Table: Breast Cancer Research and Treatment 4 Basic/Translational Research Carcinogenesis and Signaling Group 5R) Signaling in Breast Cancer 6 NF-B Family of Transcription Factors in Breast Cancer 7 Transgenic Mouse

  8. Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor

    SciTech Connect (OSTI)

    Yogesha, S.D.; Sharff, Andrew J.; Giovannini, Marco; Bricogne, Gerard; Izard, Tina (House Ear); (Globel Phasing); (Scripps)

    2014-10-02T23:59:59.000Z

    The merlin-1 tumor suppressor is encoded by the Neurofibromatosis-2 (Nf2) gene and loss-of-function Nf2 mutations lead to nervous system tumors in man and to several tumor types in mice. Merlin is an ERM (ezrin, radixin, moesin) family cytoskeletal protein that interacts with other ERM proteins and with components of cell-cell adherens junctions (AJs). Merlin stabilizes the links of AJs to the actin cytoskeleton. Thus, its loss destabilizes AJs, promoting cell migration and invasion, which in Nf2{sup +/-} mice leads to highly metastatic tumors. Paradoxically, the 'closed' conformation of merlin-1, where its N-terminal four-point-one, ezrin, radixin, moesin (FERM) domain binds to its C-terminal tail domain, directs its tumor suppressor functions. Here we report the crystal structure of the human merlin-1 head domain when crystallized in the presence of its tail domain. Remarkably, unlike other ERM head-tail interactions, this structure suggests that binding of the tail provokes dimerization and dynamic movement and unfurling of the F2 motif of the FERM domain. We conclude the 'closed' tumor suppressor conformer of merlin-1 is in fact an 'open' dimer whose functions are disabled by Nf2 mutations that disrupt this architecture.

  9. Corrosion of Ferritic-Martensitic Steels in Steam and Supercritical Water Jeremy Bischoffa, Arthur T. Mottaa, Chad Eichfeldb, Robert J. Comstockc, Guoping Caod, Todd

    E-Print Network [OSTI]

    Motta, Arthur T.

    Corrosion of Ferritic-Martensitic Steels in Steam and Supercritical Water Jeremy Bischoffa, Arthur Corrosion tests were performed in steam and supercritical water at 500°C for two ferritic- martensitic alloys: HCM12A and NF616. The corrosion kinetics for the two alloys are similar in both environments

  10. Corrosion of ferriticmartensitic steels in steam and supercritical water Jeremy Bischoff a,b

    E-Print Network [OSTI]

    Motta, Arthur T.

    Corrosion of ferritic­martensitic steels in steam and supercritical water Jeremy Bischoff a: Available online xxxx a b s t r a c t Corrosion tests were performed in steam and supercritical water at 500 °C for two ferritic­martensitic alloys: HCM12A and NF616. The corrosion kinetics for the two alloys

  11. 12/31/11 Analog to Digital Converter Test Report Page 1 of 5 Created 3/3/08 by Nicole Rodia Modified 5/18/08 11:01 PM by Nicole Rodia

    E-Print Network [OSTI]

    Ruina, Andy L.

    as well as on the Ko Brain board under a variety of conditions. Using the Ko Brain board, tests were wire Soldering iron 1.5 V battery Battery holder 10 k, 100 k resistors 22 nF capacitor Multimeter wrap wire to keep wires short to reduce electromagnetic interference. Soldered connections to battery

  12. Managed by UT-Battelle for the Department of Energy

    E-Print Network [OSTI]

    McDonald, Kirk

    Managed by UT-Battelle for the Department of Energy Neutrino Factory Mercury Containment Concepts V;2 Managed by UT-Battelle for the Department of Energy HPT Workshop, 6 Nov 2008 Outline Neutrino Factory.14 Dimensions in cm #12;6 Managed by UT-Battelle for the Department of Energy HPT Workshop, 6 Nov 2008 NF Hg Jet

  13. Managed by UT-Battelle for the Department of Energy

    E-Print Network [OSTI]

    McDonald, Kirk

    Managed by UT-Battelle for the Department of Energy Design and Operational Features of a Mercury CERN #12;2 Managed by UT-Battelle for the Department of Energy Contributors Tom McManamy, Chief Dec 08 #12;3 Managed by UT-Battelle for the Department of Energy NF-IDS Meeting 15 Dec 08 Outline

  14. W W W. N A T U R E . C O M / N A T U R E | 1 SUPPLEMENTARYINFORMATION doi:10.1038/nature13029

    E-Print Network [OSTI]

    Cai, Long

    M resistor and a 330 nF capacitor that form a low-pass filter. The two 22 M resistors prevent the drive coil in the vicinity of the ferrite inductor and the voltage on the capacitor is probed by the FET) that is known for minimized core and eddy current losses. The inductor is wound with Litz wires of optimal wire

  15. Mar. Drugs 2011, 9, 1293-1306; doi:10.3390/md9071293 Marine Drugs

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis Zografou, Athens 15771 of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-B-dependent luciferase

  16. north-south,100m east-west, 100m

    E-Print Network [OSTI]

    the identification of self-sustaining populations. Use of molecular genetic markers can aid in this identification with increasing age of a stand. Thus spatial genetic bubble Materials and Methods For DNA isolation one leaf position of Bolivar Co. (large triangle) population between the Delta NF and Arkansas populations. Other

  17. Zap! Issue 3

    E-Print Network [OSTI]

    Multiple Contributors

    1979-01-01T23:59:59.000Z

    the output of his 'Highland Mist' dispenser. I mlit;t admit Scott's achievement was a triumph of inver.i:ivE: skil1. It cO/i,bined the innocent ap])earance of an early domestic re.dio with the effectiv'nf'ss of a medieval thumb scrf'VI. Now, how...

  18. $?_S$ from Lattice QCD: progresses and perspectives for a realistic full-QCD determination of the running Strong coupling

    E-Print Network [OSTI]

    B. Blossier; Ph. Boucaud; F. De soto; M. Gravina; V. Morenas; O. Pne; J. Rodrguez-Quintero

    2010-12-14T23:59:59.000Z

    Some very recent computations of $\\alpha_{\\bar{\\rm MS}}(M_Z)$ from $N_f=1+1$ lattice simulations and of the running of the Strong coupling, obtained from the lattice ghost-gluon vertex, over a large momentum window are very briefly reviewed.

  19. Inter-Kingdom Signaling Interactions in Enterohemorrhagic Escherichia coli Infections

    E-Print Network [OSTI]

    Bansal, Tarun

    2011-10-21T23:59:59.000Z

    IL-10, decrease in the pro-inflammatory cytokine IL-8, decrease in the activity of the pro-inflammatory transcription factor NF-?B, and decrease in colonization by EHEC of the indole-pre-treated HCT-8 cells. We established that factors secreted...

  20. Dissipative cryogenic filters with zero dc resistance Hendrik Bluhma

    E-Print Network [OSTI]

    Moler, Kathryn A.

    additional capacitors of 10 nF per line, an attenuation of at least 45 dB above 10 MHz can be obtained. Thus requirements also occur for flux bias lines of super- conducting qubits. Most flux biased qubit experiments cur resistive con- ductor. The low frequency attenuation is increased by adding two discrete capacitors

  1. Physical and Mechanical characterization of soya, colza and rye seeds Cdric Degouet, Blaise Nsom, Eric Lolive and Andr Grohens

    E-Print Network [OSTI]

    Paris-Sud XI, Universit de

    of the contact and Coulomb's friction law are governed by the following relations: 0=Nd ; d and 0N (1) NfT (2 using soil mechanics tests, which are based on Coulomb's laws of friction (Costet and Sanglerat, 1975 This paper presents characterization of the following dry granular materials - soya, colza and rye seeds

  2. ENEA F. Zonca 1 Ballooning Transformation

    E-Print Network [OSTI]

    Zonca, Fulvio

    based on scale separation. 2 BF introduced by a number of authors in the late 70's (Coppi PRL77, Lee PFBW 77, Glasser PFBW 77, Pegoraro IAEA 78, Connor PRL 78, Dewar NF81) to conveniently treat linear of authors in the late 70's (Coppi PRL77, Lee PFBW 77, Glasser PFBW 77, Pegoraro IAEA 78, Connor PRL 78

  3. A proposed safety training program for industrial supervisors

    E-Print Network [OSTI]

    Loveless, Sidney Louis

    1969-01-01T23:59:59.000Z

    were selected from the 1967-68 Directory nf Texas Nanufacturers. Only firmi vhich manu- facture industrial goods were used in the study. It was felt that cnirPanics which employed fever than 100 employees i~auld not nnmally be able to justify nor...

  4. Ancient and Modern Agriculture.

    E-Print Network [OSTI]

    Short, A. K.

    1923-01-01T23:59:59.000Z

    was an expert, and the project leader, in this line of work. When the Children of Israel were sent to spy out (make a survoT7 nf l the land of Canaan (Numbers 13:l-20) each tribe was instructed point a wise man a s leader t o represent them in making...

  5. PARAMETRIC EXCITATION OF PLASMA OSCILLATIONS IN JOSEPHSON JUNCTIONS

    E-Print Network [OSTI]

    Boyer, Edmond

    b. ' FIG. 3. - (a) 03B1c tan oo vs. 2/Q at co = 2 cop. Straight line : theory. Circles : analog harmonic generation with big amplitude may be understood from a discussion of the stability properties frequency FIG. 1. - The junction model. For the analog R = 500 il, Io = 1 mA, C = 100 nF, and k = 2 e

  6. Genetic analyses of bovine CARD15, a putative disease resistance gene

    E-Print Network [OSTI]

    Taylor, Kristen Hawkins

    2004-09-30T23:59:59.000Z

    Through a binding partner the CARD15 gene activates NF-kB, a molecule with a role in the initiation of the inflammatory immune response. The gene is highly conserved in both structure and function in human and mouse and ...

  7. Genetic analyses of bovine CARD15, a putative disease resistance gene

    E-Print Network [OSTI]

    Taylor, Kristen Hawkins

    2004-09-30T23:59:59.000Z

    Through a binding partner the CARD15 gene activates NF-kB, a molecule with a role in the initiation of the inflammatory immune response. The gene is highly conserved in both structure and function in human and mouse and has recently been implicated...

  8. Annual Colorado Rare Plant Symposium: G1 Plants of Colorado

    E-Print Network [OSTI]

    leaves San Juan NF revising Management Plan make sure it is included, although it occurs on the Rio (Tamara Naumann) has no resources to survey for this species, focus is on weed management. CNE population. Can survey for this species almost any time of year. In Arches NP, sand blowing over the plants

  9. Dysregulated Toll-like receptor expression and signaling in bone marrow-derived macrophages at

    E-Print Network [OSTI]

    Toledo, University of

    . McInerney1 1 Department of Medicinal and Biological Chemistry, College of Pharmacy, and 2 Department Center, Walther Cancer Institute, Indianapolis, IN, USA 5 Present address: College of Pharmacy in the diabetic state correlated with increased nuclear factor kappa B (NF-jB) activation in response to the TLR4

  10. Timed CSP and Object-Z John Derrick

    E-Print Network [OSTI]

    Kent, University of

    Timed CSP and Object-Z John Derrick Computing Laboratory, University of Kent, Canterbury, CT2 7NF a simple integration of timed CSP and Object-Z. Following existing work, the components in such an inte- gration are written as either Object-Z classes, or timed CSP processes, and are combined together using

  11. SANDY MAGNUS, MS-1 (BLUE) Meal Day 1* Day 2 Day 3 Day 4 Day 5 Day 6

    E-Print Network [OSTI]

    Beans Fiesta Chicken (T) Broccoli au Gratin (R) Macaroni & Cheese (R) Red Beans & Rice (T) Crackers (NF) x2 Creamed Spinach (R) Black Beans (T) Creamed Spinach (R) Tortillas (FF) x2 Creamed Spinach (R) Black Beans (T) Tortillas (FF) x2 Tomatoes & Eggplant (T) Tortillas (FF) x2 Cauliflower w/ Cheese (R

  12. The effects of ethylenediurea and sodium erythorbate on photosynthetic function of ozone-exposed loblolly pine seedlings

    E-Print Network [OSTI]

    Kuehler, Eric Anthony

    1995-01-01T23:59:59.000Z

    -top chambers in east Texas for one growing season beginning in April 1994 while being exposed to either sub-ambient (CF), approximate ambient (NF), 1.5Y,, 2.OX, or 2.5X ambient ozone levels. Net photosynthesis (A), stomatal conductance (g), and chloroplast...

  13. VALIDATION DE METHODE D'ETALONNAGE ET EVALUATION DES INCERTITUDES DE MESURE EN ANALYSE

    E-Print Network [OSTI]

    Boyer, Edmond

    'INERIS, organisme accrdit ISO/CEI/17025, certifi ISO 9001 et reconnu BPL, a pour mission la matrise des risques mesure exposs dans ce document. Abstract : INERIS, a certified (ISO 9001) and accredited (ISO/CEI 17025 norme NF ISO 17993 [5]. Le diagramme d'Ishikawa de l'analyse du processus de mesure des HAP par CLHP

  14. TLR9 Immune Evasion by Murine Gammaherpesvirus 68

    E-Print Network [OSTI]

    Pezda, Andrea Christiane

    2010-01-01T23:59:59.000Z

    MOI MyD88 NF-?B NLR ODN ORF PAMP pDC PDMS PFU PRRs RIG-I RLRupon infection of cDCs and pDC. The former but not thegenome-positive cells in pDC populations isolated from mice

  15. Interim Design Report

    E-Print Network [OSTI]

    R. J. Abrams; S. K. Agarwalla; A. Alekou; C. Andreopoulos; C. M. Ankenbrandt; S. Antusch; M. Apollonio; M. Aslaninejad; J. Back; P. Ballett; G. Barker; K. B. Beard; E. Benedetto; J. R. J. Bennett; J. S. Berg; S. Bhattacharya; V. Blackmore; M. Blennow; A. Blondel; A. Bogacz; M. Bonesini; C. Bontoiu; C. Booth; C. Bromberg; S. Brooks; A. Bross; O. Caretta; A. Cervera-Villanueva; S. Choubey; D. Cline; J. Cobb; P. Coloma; L. Coney; M. A. C. Cummings; T. Davenne; A. de Gouvea; C. Densham; X. Ding; A. Donini; P. Dornan; M. Dracos; F. Dufour; R. Eccleston; R. Edgecock; I. Efthymiopoulos; M. Ellis; E. Fernandez-Martinez; R. Fernow; G. Flanagan; J. C. Gallardo; R. Gandhi; R. Garoby; B. Gavela; S. Geer; S. Gilardoni; J. J. Gomez-Cadenas; S. Goswami; V. B. Graves; R. Gupta; G. Hanson; P. Harrison; T. Hart; P. Hernandez; P. Huber; D. Indumathi; R. P. Johnson; C. Johnstone; Y. Karadzhov; D. Kelliher; H. Kirk; J. Kopp; Y. Kudenko; Y. Kuno; A. Kurup; P. Kyberd; A. Laing; T. Li; M. Lindner; K. Long; J. Lopez Pavon; P. Loveridge; S. Machida; D. Majumdar; M. Maltoni; J. Martin-Albo; M. Martini; R. Matev; K. T. McDonald; A. McFarland; D. Meloni; M. Mezzetto; P. Migliozzi; S. R. Mishra; N. Mokhov; N. Mondal; J. Morfin; Y. Mori; V. Morozov; D. Neuffer; T. Ota; V. Palladino; S. Parke; S. Pascoli; J. Pasternak; J. Peltoniemi; R. Petti; T. Planche; M. Popovic; J. Pozimski; G. Prior; C. Prior; G. Rees; S. Rigolin; T. J. Roberts; Y. Roblin; C. Rogers; R. Samulyak; T. Schwetz; N. Simos; N. Sinha; G. Skoro; P. Snopok; F. J. P. Soler; N. Souchlas; J. Strait; D. Stratakis; S. Striganov; J. Tang; J. W. G. Thomason; L. Tortora; R. Tsenov; W. Winter; H. Witte; O. Yasuda; C. Y. Yoshikawa; M. Zisman

    2011-12-13T23:59:59.000Z

    The International Design Study for the Neutrino Factory (the IDS-NF) was established by the community at the ninth "International Workshop on Neutrino Factories, super-beams, and beta- beams" which was held in Okayama in August 2007. The IDS-NF mandate is to deliver the Reference Design Report (RDR) for the facility on the timescale of 2012/13. In addition, the mandate for the study [3] requires an Interim Design Report to be delivered midway through the project as a step on the way to the RDR. This document, the IDR, has two functions: it marks the point in the IDS-NF at which the emphasis turns to the engineering studies required to deliver the RDR and it documents baseline concepts for the accelerator complex, the neutrino detectors, and the instrumentation systems. The IDS-NF is, in essence, a site-independent study. Example sites, CERN, FNAL, and RAL, have been identified to allow site-specific issues to be addressed in the cost analysis that will be presented in the RDR. The choice of example sites should not be interpreted as implying a preferred choice of site for the facility.

  16. KT McDonald Muon Accelerator Program DoE Review (FNAL) August 30, 2012 1 Target and Absorbers

    E-Print Network [OSTI]

    McDonald, Kirk

    , 2012 2 Mission Target: Maximum production of of energies ~ 100-400 MeV from a 4-MW proton beam (E: Absorb primary proton beam in liquid-metal pool. Absorb secondary particles in He-gas-cooled tungsten In the IDS-NF costing scenario, the Target System includes the production target and the magnetized pion

  17. HES5 silencing is an early and recurrent change in prostate tumourigenesis

    E-Print Network [OSTI]

    Massie, Charles E; Spiteri, Inmaculada; Ross-Adams, Helen; Luxton, Hayley; Kay, Jonathan; Whitaker, Hayley C; Dunning, Mark J; Lamb, Alastair\tD; Ramos-Montoya, Antonio; Brewer, Daniel\tS; Cooper, Colin S.; Eeles, Rosalind; UK\tProstate\tICGC\tGroup; Warren, Anne\tY; Tavar, Simon; Neal, David\tE.; Lynch, Andy G

    2015-01-05T23:59:59.000Z

    potential effector mechanism of HES5 silencingERC-14-0454. http://erc.endocrinology-journals.org DOI: 10.1530/ERC-14-0454 q 2015 The authors Printed in Great Britainmatics/btu049) Austin DW, Allen MS, McCollum JM, Dar RD, Wilgus JR, Sayler GS, Samatova NF...

  18. High glucose induces inflammatory cytokine through protein kinase C-induced toll-like receptor 2 pathway in gingival fibroblasts

    SciTech Connect (OSTI)

    Jiang, Shao-Yun, E-mail: jiangshaoyun@yahoo.com [School of Dentistry, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin 300070 (China)] [School of Dentistry, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin 300070 (China); Wei, Cong-Cong; Shang, Ting-Ting; Lian, Qi; Wu, Chen-Xuan [School of Dentistry, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin 300070 (China)] [School of Dentistry, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin 300070 (China); Deng, Jia-Yin, E-mail: yazhou2991@126.com [School of Dentistry, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin 300070 (China)] [School of Dentistry, Tianjin Medical University, 12 Qi Xiang Tai Street, Heping District, Tianjin 300070 (China)

    2012-10-26T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer High glucose significantly induced TLR2 expression in gingival fibroblasts. Black-Right-Pointing-Pointer High glucose increased NF-{kappa}B p65 nuclear activity, IL-1{beta} and TNF-{alpha} levels. Black-Right-Pointing-Pointer PKC-{alpha}/{delta}-TLR2 pathway is involved in periodontal inflammation under high glucose. -- Abstract: Toll-like receptors (TLRs) play a key role in innate immune response and inflammation, especially in periodontitis. Meanwhile, hyperglycemia can induce inflammation in diabetes complications. However, the activity of TLRs in periodontitis complicated with hyperglycemia is still unclear. In the present study, high glucose (25 mmol/l) significantly induced TLR2 expression in gingival fibroblasts (p < 0.05). Also, high glucose increased nuclear factor kappa B (NF-{kappa}B) p65 nuclear activity, tumor necrosis factor-{alpha} (TNF-{alpha}) and interleukin-l{beta} (IL-1{beta}) levels. Protein kinase C (PKC)-{alpha} and {delta} knockdown with siRNA significantly decreased TLR2 and NF-{kappa}B p65 expression (p < 0.05), whereas inhibition of PKC-{beta} had no effect on TLR2 and NF-{kappa}B p65 under high glucose (p < 0.05). Additional studies revealed that TLR2 knockdown significantly abrogated high-glucose-induced NF-{kappa}B expression and inflammatory cytokine secretion. Collectively, these data suggest that high glucose stimulates TNF-{alpha} and IL-1{beta} secretion via inducing TLR2 through PKC-{alpha} and PKC-{delta} in human gingival fibroblasts.

  19. Silencing MR-1 attenuates inflammatory damage in mice heart induced by AngII

    SciTech Connect (OSTI)

    Dai, Wenjian [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China) [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China); Hunan Environment-Biological Polytechnic College, Hengyang Hunan 421005 (China); Chen, Haiyang [Hunan Environment-Biological Polytechnic College, Hengyang Hunan 421005 (China)] [Hunan Environment-Biological Polytechnic College, Hengyang Hunan 421005 (China); Jiang, Jiandong [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China)] [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China); Kong, Weijia, E-mail: wjkong894@sohu.com [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China)] [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China); Wang, Yiguang, E-mail: wangyh456@yahoo.com.cn [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China)] [Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Key Lab of Antibiotic Biotechnology, Ministry of Health, Beijing 100050 (China)

    2010-01-15T23:59:59.000Z

    Myofibrillogenesis regulator-1(MR-1) can aggravate cardiac hypertrophy induced by angiotensin(Ang) II in mice through activation of NF-{kappa}B signaling pathway, and nuclear transcription factor (NF)-{kappa}B and activator protein-1(AP-1) regulate inflammatory and immune responses by increasing the expression of specific inflammatory genes in various tissues including heart. Whether inhibition of MR-1 expression will attenuate AngII-induced inflammatory injury in mice heart has not been explored. Herein, we monitored the activation of NF-{kappa}B and AP-1, together with expression of pro-inflammatory of interleukin(IL)-6, tumor necrosis factor(TNF)-{alpha}, vascular-cell adhesion molecule (VCAM)-1, platelet endothelial cell adhesion molecule (PECAM), and inflammatory cell infiltration in heart of mice which are induced firstly by AngII (PBS),then received MR-1-siRNA or control-siRNA injecting. We found that the activation of NF-{kappa}B and AP-1 was inhibited significantly, together with the decreased expression of IL-6, TNF-{alpha}, VCAM-1, and PECAM in AngII-induced mice myocardium in MR-1-siRNA injection groups compared with control-siRNA injecting groups. However, the expression level of MR-1 was not an apparent change in PBS-infused groups than in unoperation groups, and MR-1-siRNA do not affect the expression of MR-1 in PBS-infused mice. Our findings suggest that silencing MR-1 protected mice myocardium against inflammatory injury induced by AngII by suppression of pro-inflammatory transcription factors NF-{kappa}B and AP-1 signaling pathway.

  20. NYPA/TH!NK Clean Commute Program Final Report - Inception through December 2004

    SciTech Connect (OSTI)

    James Francfort; Don Karner

    2005-11-01T23:59:59.000Z

    The Clean Commute Program uses TH!NK city electric vehicles from Ford Motor Companys electric vehicle group, TH!NK Mobility, to demonstrate the feasibility of using electric transportation in urban applications. Suburban New York City railroad commuters use the TH!NK city vehicles to commute from their private residences to railroad stations, where they catch commuter trains into New York City. Electric vehicle charging infrastructure for the TH!NK city vehicles is located at the commuters private residences as well as seven train stations. Ford leased at total of 97 TH!NK city electric vehicles to commuters from Westchester, Putnam, Rockland, Queens, Nassau, and Suffolk counties for $199 per month. First Clean Commute Program vehicle deliveries occurred late in 2001, with data collection commencing in February 2002. Through May, 2004, 24 of the lessees have returned their vehicles to Ford and no longer participate in the Clean Commute Program. Reasons given for leaving the Program include relocation out of the Program area, change in employment status, change in commuting status, and, in a few cases, dissatisfaction with the vehicle. Additionally, 13 vehicles were returned to Ford when the lease was completed. In August 2002, Ford announced that it was ceasing production of the TH!NK city and would not extend any TH!NK city leases. Mileage accumulation dropped in the last quarter of the program as vehicle leases were returned to Ford. The impact of the program overall was significant as participants in the Clean Commute Program drove their vehicles over 406,074 miles, avoiding the use of over 18,887 gallons of gasoline. During the active portion of the program, the TH!NK city vehicles were driven an average of between 180 and 230 miles per month. Over 95% of all trips taken with the TH!NK city vehicles replaced trips previously taken in gasoline vehicles. This report covers the period from Program inception through December 2004.

  1. NYPA/TH!NK Clean Commute Program Report Inception Through May 2004

    SciTech Connect (OSTI)

    Don Karner; James Francfort; Randall Solomon

    2004-11-01T23:59:59.000Z

    The Clean Commute Program uses TH!NK city electric vehicles from Ford Motor Companys electric vehicle group, TH!NK Mobility, to demonstrate the feasibility of using electric vehicles for transportation in urban applications. Suburban New York City railroad commuters use the TH!NK city vehicles to commute from their private residences to railroad stations, where they catch commuter trains into New York City. Electric vehicle charging infrastructure for the TH!NK city vehicles is located at the commuters private residences as well as seven train stations. Ford leased 97 TH!NK city electric vehicles to commuters from Westchester, Putnam, Rockland, Queens, Nassau, and Suffolk counties for $199 per month per vehicle. The first Clean Commute Program vehicle deliveries occurred late in 2001, with data collection commencing in February 2002. Through May 2004, 24 of the lessees have returned their vehicles to Ford and no longer participate in the Clean Commute Program. Reasons given for returning the vehicles include relocation out of the Program area, change in employment status, change in commuting status, and, in a few cases, dissatisfaction with the vehicle. Additionally, 13 vehicles have been returned to Ford as their leases have completed. In August 2002, Ford announced that it was ceasing production of the TH!NK city and would not extend any TH!NK city leases. Through May 2004, participants in the Clean Commute Program have driven their vehicles over 370,000 miles, avoiding the use of over 17,000 gallons of gasoline. The TH!NK city vehicles are driven an average of between 180 and 230 miles per month, and over 95% of all trips taken with the TH!NK city vehicles replace trips previously taken in gasoline vehicles. This report covers the period from Program inception through May 2004.

  2. Baseline Environmental Analysis Report for the K-1251 Barge Facility at the East Tennessee Technology Park, Oak Ridge, Tennessee

    SciTech Connect (OSTI)

    Van Winkle J.E.

    2007-08-24T23:59:59.000Z

    This report documents the baseline environmental conditions of the U. S. Department of Energy's (DOE's) K-1251 Barge Facility, which is located at the East Tennessee Technology Park (ETTP). DOE is proposing to lease the facility to the Community Reuse Organization of East Tennessee (CROET). This report provides supporting information for the use, by a potential lessee, of government-owned facilities at ETTP. This report is based upon the requirements of Sect. 120(h) of the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). The lease footprint is slightly over 1 acre. The majority of the lease footprint is defined by a perimeter fence that surrounds a gravel-covered area with a small concrete pad within it. Also included is a gravel drive with locked gates at each end that extends on the east side to South First Avenue, providing access to the facility. The facility is located along the Clinch River and an inlet of the river that forms its southern boundary. To the east, west, and north, the lease footprint is surrounded by DOE property. Preparation of this report included the review of government records, title documents, historic aerial photos, visual and physical inspections of the property and adjacent properties, and interviews with current and former employees involved in the operations on the real property to identify any areas on the property where hazardous substances and petroleum products or their derivatives and acutely hazardous wastes were known to have been released or disposed. Radiological surveys were conducted and chemical samples were collected to assess the facility's condition.

  3. Nicotine stimulates urokinase-type plasminogen activator receptor expression and cell invasiveness through mitogen-activated protein kinase and reactive oxygen species signaling in ECV304 endothelial cells

    SciTech Connect (OSTI)

    Khoi, Pham Ngoc; Park, Jung Sun; Kim, Nam Ho; Jung, Young Do, E-mail: ydjung@chonnam.ac.kr

    2012-03-01T23:59:59.000Z

    Urokinase-type plasminogen activator receptor (uPAR) expression is elevated during inflammation, tissue remodeling and in many human cancers. This study investigated the effect of nicotine, a major alkaloid in tobacco, on uPAR expression and cell invasiveness in ECV304 endothelial cells. Nicotine stimulated uPAR expression in a dose-dependent manner and activated extracellular signal-regulated kinases-1/2 (Erk-1/2), c-Jun amino-terminal kinase (JNK) and p38 mitogen activated protein kinase (MAPK). Specific inhibitors of MEK-1 (PD98059) and JNK (SP600125) inhibited the nicotine-induced uPAR expression, while the p38 MAPK inhibitor SB203580 did not. Expression vectors encoding dominant negative MEK-1 (pMCL-K97M) and JNK (TAM67) also prevented nicotine-induced uPAR promoter activity. The intracellular hydrogen peroxide (H{sub 2}O{sub 2}) content was increased by nicotine treatment. The antioxidant N-acetylcysteine prevented nicotine-activated production of reactive oxygen species (ROS) and uPAR expression. Furthermore, exogenous H{sub 2}O{sub 2} increased uPAR mRNA expression. Deleted and site-directed mutagenesis demonstrated the involvement of the binding sites of transcription factor nuclear factor-kappaB (NF-?B) and activator protein (AP)-1 in the nicotine-induced uPAR expression. Studies with expression vectors encoding mutated NF-?B signaling molecules and AP-1 decoy confirmed that NF-?B and AP-1 were essential for the nicotine-stimulated uPAR expression. MAPK (Erk-1/2 and JNK) and ROS functioned as upstream signaling molecules in the activation of AP-1 and NF-?B, respectively. In addition, ECV304 endothelial cells treated with nicotine displayed markedly enhanced invasiveness, which was partially abrogated by uPAR neutralizing antibodies. The data indicate that nicotine induces uPAR expression via the MAPK/AP-1 and ROS/NF-?B signaling pathways and, in turn, stimulates invasiveness in human ECV304 endothelial cells. -- Highlights: ? Endothelial cells treated with nicotine displayed enhanced invasiveness. ? Nicotine induces uPAR expression and, in turn, stimulates invasiveness. ? MAPK/AP-1 and ROS/NF-?B signals are involved in nicotine-induced uPAR.

  4. Quantum Critical Behaviour in a Graphene-like Model

    E-Print Network [OSTI]

    Simon Hands; Costas Strouthos

    2008-06-30T23:59:59.000Z

    We present the first results of numerical simulations of a 2+1 dimensional fermion field theory based on a recent proposal for a model of graphene, consisting of N_f four-component Dirac fermions moving in the plane and interacting via an instantaneous Coulomb interaction. In the strong-coupling limit we identify a critical number of flavors N_fc=4.8(2) separating an insulating from a conducting phase. This transition corresponds to the location of a quantum critical point, and we use a fit to the equation of state for the chiral order parameter to estimate the critical exponents. Next we simulate N_f=2 corresponding to real graphene, and approximately locate a transition from strong to weak coupling behaviour. Strong correlations are evident in the weak-coupling regime.

  5. A compact method for matrix decomposition matrix inversion, and solution of simultaneous linear equations

    E-Print Network [OSTI]

    Banker, Donald Henry

    1966-01-01T23:59:59.000Z

    t K)()A (Ks K) ~ 62 ' 61 ~ K)+At Ie I ) )12 ~ K)-A( JsK)%A( I s J) eK)()A(f e I ) 920 TNRU 70 COMPUTES U INVERSE Nf ~N+I DO 70 J~f tN JI +'Nf IF&N J)301 &76&73 DO 71 K~ I ~ N K 1 ~ J I-K IF ( Jl-K ) 301 ~ '70 ~ 72 A(KI ~ Jf )~-A(KI ~ Jl... & i & n; 1 & j & n&1; ai 1 ai 1 where il is any specific choice (1) (0) 1 1 of the integers 1 to n such that ai 1 $0; 1 a ~ / for 1 & j & n ilj i. ll and aj j aij for i p il, 1 & i & n~ 1 & j & n. Let (2. 17) A ~ (aij ) for 1 & i, k & n; and 1...

  6. Mott multicriticality of Dirac electrons in graphene

    E-Print Network [OSTI]

    Classen, Laura; Janssen, Lukas; Scherer, Michael M

    2015-01-01T23:59:59.000Z

    We study the multicritical behavior for the semimetal-insulator transitions on graphene's honeycomb lattice using the Gross-Neveu-Yukawa effective theory with two order parameters: the SO(3) (Heisenberg) order parameter describes the antiferromagnetic transition, and the $\\mathbb{Z}_2$ (Ising) order parameter describes the transition to a staggered density state. Their coupling induces multicritical behavior which determines the structure of the phase diagram close to the multicritical point. Depending on the number of fermion flavors $N_f$ and working in the perturbative regime in vicinity of three (spatial) dimensions, we observe first order or continuous phase transitions at the multicritical point. For the graphene case of $N_f=2$ and within our low order approximation, the phase diagram displays a tetracritical structure.

  7. Design and development of a high-temperature sodium compatibility testing facility

    SciTech Connect (OSTI)

    Hvasta, M. G.; Nolet, B. K.; Anderson, M. H. [Univ. of Wisconsin-Madison, 1500 Engineering Dr., Madison - ERB 841, WI 53705 (United States)

    2012-07-01T23:59:59.000Z

    The use of advanced alloys within sodium-cooled fast reactors (SFRs) has been identified as a means of increasing plant efficiency and reducing construction costs. In particular, alloys such as NF-616, NF-709 and HT-UPS are promising because they exhibit greater strength than traditional structural materials such as 316-SS. However, almost nothing is known about the sodium compatibility of these new alloys. Therefore, research taking place at the Univ. of Wisconsin-Madison is focused on studying the effects of sodium corrosion on these materials under prototypic SFR operating conditions (600 [ deg. C], V Na=10 [m/s], C 0{approx} 1 [wppm]). This paper focuses on the design and construction of the testing facility with an emphasis on moving magnet pumps (MMPs). Corrosion data from a preliminary 500 [hr] natural convection test will also be presented. (authors)

  8. Design and Syntheses of Dyes for Biological Applications

    E-Print Network [OSTI]

    Thivierge, Cliferson

    2012-07-16T23:59:59.000Z

    NF2BN N NH N HN N F2B N 5 N NH N HN NF2B N N F2B N 6 mes mes N NH N HN R R R R R = N B F2 N N F2B N 7 Figure 1.6 Continued. 15 Lindsey investigated the potential of systems like 1 as ?optoelectronic gates... by their inherent hydrophobicities. N BF2 N R = 8 !abs max 504 nm !em max 544 nm ETE 97 % R N BF2 N R N B F2 N O N H HO2C 9 !abs max 504 nm !em max 545 nm ETE 94 % R N B F2 N O SucO OMeMeO 10 !abs max 504 nm !em max 632 nm...

  9. Low-energy neutrino factory design

    SciTech Connect (OSTI)

    Ankenbrandt, C.; /Fermilab /MUONS Inc., Batavia; Bogacz, S.A.; /Jefferson Lab; Bross, A.; Geer, S.; Johnstone, C.; Neuffer, D.; Popovic, M.; /Fermilab

    2009-07-01T23:59:59.000Z

    The design of a low-energy (4 GeV) neutrino factory (NF) is described, along with its expected performance. The neutrino factory uses a high-energy proton beam to produce charged pions. The {pi}{sup {+-}} decay to produce muons ({mu}{sup {+-}}), which are collected, accelerated, and stored in a ring with long straight sections. Muons decaying in the straight sections produce neutrino beams. The scheme is based on previous designs for higher energy neutrino factories, but has an improved bunching and phase rotation system, and new acceleration, storage ring, and detector schemes tailored to the needs of the lower energy facility. Our simulations suggest that the NF scheme we describe can produce neutrino beams generated by {approx} 1.4 x 10{sup 21} {mu}{sup +} per year decaying in a long straight section of the storage ring, and a similar number of {mu}{sup -} decays.

  10. Neutrino Factories

    SciTech Connect (OSTI)

    Geer, Steve; /Fermilab

    2010-01-01T23:59:59.000Z

    Over the last decade there has been significant progress in developing the concepts and technologies needed to produce, capture and accelerate O(10{sup 21}) muons/year. This prepares the way for a Neutrino Factory (NF) in which high energy muons decay within the straight sections of a storage ring to produce a beam of neutrinos and anti-neutrinos. The NF concept was proposed in 1997 at a time when the discovery that the three known types of neutrino ({nu}{sub e}, {nu}{sub {mu}}, {nu}{sub {tau}}) can change their flavor as they propagate through space (neutrino oscillations) was providing a first glimpse of physics beyond the Standard Model. This development prepares the way for a new type of neutrino source: a Neutrino Factory. This article reviews the motivation, design and R&D for a Neutrino Factory.

  11. Webs of Walls

    E-Print Network [OSTI]

    Minoru Eto; Youichi Isozumi; Muneto Nitta; Keisuke Ohashi; Norisuke Sakai

    2005-06-20T23:59:59.000Z

    Webs of domain walls are constructed as 1/4 BPS states in d=4, N=2 supersymmetric U(Nc) gauge theories with Nf hypermultiplets in the fundamental representation. Web of walls can contain any numbers of external legs and loops like (p,q) string/5-brane webs. We find the moduli space M of a 1/4 BPS equation for wall webs to be the complex Grassmann manifold. When moduli spaces of 1/2 BPS states (parallel walls) and the vacua are removed from M, the non-compact moduli space of genuine 1/4 BPS wall webs is obtained. All the solutions are obtained explicitly and exactly in the strong gauge coupling limit. In the case of Abelian gauge theory, we work out the correspondence between configurations of wall web and the moduli space CP^{Nf-1}.

  12. Structure of a NEMO/IKK-Associating Domain Reveals Architecture of the Interaction Site

    SciTech Connect (OSTI)

    Rushe,M.; Silvian, L.; Bixler, S.; Chen, L.; Cheung, A.; Bowes, S.; Cuervo, H.; Berkowitz, S.; Zheng, T.; et al

    2008-01-01T23:59:59.000Z

    The phosphorylation of I{kappa}B by the IKK complex targets it for degradation and releases NF-{kappa}B for translocation into the nucleus to initiate the inflammatory response, cell proliferation, or cell differentiation. The IKK complex is composed of the catalytic IKK{alpha}/{beta} kinases and a regulatory protein, NF-{kappa}B essential modulator (NEMO; IKK{gamma}). NEMO associates with the unphosphorylated IKK kinase C termini and activates the IKK complex's catalytic activity. However, detailed structural information about the NEMO/IKK interaction is lacking. In this study, we have identified the minimal requirements for NEMO and IKK kinase association using a variety of biophysical techniques and have solved two crystal structures of the minimal NEMO/IKK kinase associating domains. We demonstrate that the NEMO core domain is a dimer that binds two IKK fragments and identify energetic hot spots that can be exploited to inhibit IKK complex formation with a therapeutic agent.

  13. Superconducting and Spinning Non-Abelian Flux Tubes

    E-Print Network [OSTI]

    Y. Brihaye; Y. Verbin

    2008-04-11T23:59:59.000Z

    We find new non-Abelian flux tube solutions in a model of $N_f$ scalar fields in the fundamental representation of SU(N)xU(1) with $N \\leq N_f$ (the ``extended non-Abelian Higgs model''), and study their main properties. Among the solutions there are spinning strings as well as superconducting ones. The solutions exist only in a non trivial domain of the parameter space defined by the ratio between the SU(N) and U(1) coupling constants, the scalar self-interaction coupling constants, the magnetic fluxes (Abelian as well as non-Abelian) and the ``twist parameter'' which is a non-trivial relative phase of the Higgs fields.

  14. Quantum Phase Transition in a Graphene Model

    E-Print Network [OSTI]

    Simon Hands; Costas Strouthos

    2008-08-20T23:59:59.000Z

    We present results for the equation of state of a graphene-like model in an effort to understand the properties of its quantum phase transition. The N_f fermion species interact through a three dimensional instantaneous Coulomb potential. Since there are no reliable analytical tools that work for all values of N_f and the coupling constant g, we rely on Monte Carlo simulations to calculate the critical properties of the model near the phase transition. We consider the four-component formulation for the fermion fields, which arises naturally as the continuum limit of the staggered fermion construction in (2+1) dimensions. In the limit of infinitely strong Coulomb interaction, the system undergoes a quantum phase transition at a critical number of fermion species N_fc ~ 4.7. We also calculate the values of the critical exponents at the quantum phase transition.

  15. Field Notes, Idaho, Borell & Orr (1930-1932)

    E-Print Network [OSTI]

    Davis, William B.

    2012-04-09T23:59:59.000Z

    -y nkimsi, ;, Utrsj-y nkimsi, Knn? nkimsi, ?, Lfnjtfsto, w?, )tfonl-y nkimsi, ww, Fkindn?syt imsi, w, L N ? I q-, rnk?ytb?-ffty oaniqklp sl j-ot,,,,,,,,,,,,,,,,,,,,, ,,,,,,,,, w xtf d-l s?sys-f? ?-ffty mnf oaniqklp ,5vp ,5?p nf ,1w?,,,,,,,,,,,,,,,w xtf...-j? utyuti ?-j?qfnklr,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,w )tf )-s Ka-lqslq ?-q,,,,, ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,w )tf )-? K-dtyba-sf ?fkoa,,,,,,,,,,,,,,, w,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,yxtfx-s...

  16. Genetic analysis of equine 2', 5'-oligoadenylate synthetase (OASI) and ribonculclease L (RNASEL) polymorphims and association to severe West Nile Virus disease

    E-Print Network [OSTI]

    Rios, Jonathan Joseph

    2009-05-15T23:59:59.000Z

    dog displaying a low titer viremia [7]. Figure 1.2 Biochemical functions of some IFN-inducible proteins (Source: Samuel, 2002). Interferon activates expression of many antiviral pathways. The OAS/RNASEL and EIF2AK2 (PKR) pathways... of IFN system evasion (Source: Grandvaux, 2002) Virus Viral Protein Mechanism of IFN system inhibition Molluscum contagiosum virus MC159L Blocks EIF2AK2-mediated apoptosis[8] African swine fever virus - Inhibits NF-#1;B: encodes an NFKBIA...

  17. Comments on the U(1) axial symmetry and the chiral transition in QCD

    E-Print Network [OSTI]

    Enrico Meggiolaro

    2014-10-14T23:59:59.000Z

    We analyze (using a chiral effective Lagrangian model) the scalar and pseudoscalar meson mass spectrum of QCD at finite temperature, above the chiral transition at $T_c$, looking, in particular, for signatures of a possible breaking of the $U(1)$ axial symmetry above $T_c$. A detailed comparison between the case with a number of light quark flavors $N_f\\geq 3$ and the (remarkably different) case $N_f=2$ is performed.

  18. EIS-0350-S1: Supplemental Environmental Impact Statement for the Nuclear Facility Portion of the Chemistry and Metallurgy Research Building Replacement Project at Los Alamos National Laboratory, New Mexico

    Broader source: Energy.gov [DOE]

    This Supplemental EIS evaluates the completion of the Chemistry and Metallurgy Research Building Replacement (CMRR) Project, which consists of constructing the nuclear facility portion (CMRR-NF) at Los Alamos National Laboratory (LANL). The CMRR Project provides the analytical chemistry and materials characterization capabilities currently or previously performed in the existing Chemistry and Metallurgy Research (CMR) Building. Because of recent detailed site geotechnical investigations, certain aspects of the CMRR-NR project have changed resulting in change to the environmental impacts.

  19. OSP WEEKLY FUNDING BULLETIN Volume 5, Issue 39 September 26, 2011

    E-Print Network [OSTI]

    Alabama in Huntsville, University of

    -04 CFDA No. 12.431 Due Date: Extends due date to 31 December 2011 http://www07.grants.gov) Solicitation No. W911NF-07-R-0001-05 CFDA No. 12.431 Due Date: Extends due date to 31 December 2011 http. ONRBAA11-031 CFDA No. 12.300 Due Date: White Papers: November 1, 2011 Full Proposals: January 10, 2012

  20. A Multiple Integral Evaluation Inspired by the MultiWZ Akalu Tefera

    E-Print Network [OSTI]

    Tefera, Akalu

    A Multiple Integral Evaluation Inspired by the Multi­WZ Method 1 Akalu Tefera Department will appear in the author's Ph.D. thesis. 2 available from http \\Gamma k X j=1 D x j :R j annihilates F ,i.e., I X i=0 a i (n)F (n + i; m;y; x) \\Gamma k X j=1 D x j (R j

  1. Geometrical structure of excited A? sstate of formaldehyde

    E-Print Network [OSTI]

    Jones, Victor Thomas

    1966-01-01T23:59:59.000Z

    , These coor dinates are mr-pmn x m (A-15 (A &6) where m - m + m +, "m ~ n c Nt' nnw con" l, m&ct. a fixn~l hn!Iy set, nf ram tnsinn axis whn, e nr'l pin is ]neat~ 1 at. the c. m? the x-axis talcen paral lnl in tI? 0-0 t~onrl ~ as shown in Fi p...

  2. Universidad Autonoma de Madrid Algebra Lineal. Curso 2010-11 Matematicas

    E-Print Network [OSTI]

    Gonzlez Dez, Gabino

    Universidad Autonoma de Madrid Algebra Lineal. Curso 2010-11 Matematicas ALGEBRA LINEAL Hoja 7 respecto de B) coincide con la aplicacion lineal f(x, y, z) = x - y. Encontrar bases de Ker(f) = N(f) y de Im f. Comprobar la formula de la dimension. 2. Sea f : M22 R3 la aplicacion lineal dada por f

  3. Universidad Autonoma de Madrid Algebra Lineal. Curso 2011-12 Matematicas

    E-Print Network [OSTI]

    Gonzlez Dez, Gabino

    Universidad Autonoma de Madrid Algebra Lineal. Curso 2011-12 Matematicas ALGEBRA LINEAL Hoja 8 respecto de B) coincide con la aplicacion lineal f(x, y, z) = x - y. Encontrar bases de Ker(f) = N(f) y de Im f. Comprobar la formula de la dimension. 2. Sea f : M22 R3 la aplicacion lineal dada por f

  4. Spleen tyrosine kinase mediates high glucose-induced transforming growth factor-{beta}1 up-regulation in proximal tubular epithelial cells

    SciTech Connect (OSTI)

    Yang, Won Seok; Chang, Jai Won [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); Han, Nam Jeong [Department of Cell Biology, Asan Institute for Life Sciences, Seoul (Korea, Republic of)] [Department of Cell Biology, Asan Institute for Life Sciences, Seoul (Korea, Republic of); Lee, Sang Koo [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); Park, Su-Kil, E-mail: skpark@amc.seoul.kr [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)

    2012-09-10T23:59:59.000Z

    The role of spleen tyrosine kinase (Syk) in high glucose-induced intracellular signal transduction has yet to be elucidated. We investigated whether Syk is implicated in high glucose-induced transforming growth factor-{beta}1 (TGF-{beta}1) up-regulation in cultured human proximal tubular epithelial cells (HK-2 cell). High glucose increased TGF-{beta}1 gene expression through Syk, extracellular signal-regulated kinase (ERK), AP-1 and NF-{kappa}B. High glucose-induced AP-1 DNA binding activity was decreased by Syk inhibitors and U0126 (an ERK inhibitor). Syk inhibitors suppressed high glucose-induced ERK activation, whereas U0126 had no effect on Syk activation. High glucose-induced NF-{kappa}B DNA binding activity was also decreased by Syk inhibitors. High glucose increased nuclear translocation of p65 without serine phosphorylation of I{kappa}B{alpha} and without degradation of I{kappa}B{alpha}, but with an increase in tyrosine phosphorylation of I{kappa}B{alpha} that may account for the activation of NF-{kappa}B. Both Syk inhibitors and Syk-siRNA attenuated high glucose-induced I{kappa}B{alpha} tyrosine phosphorylation and p65 nuclear translocation. Depletion of p21-activated kinase 2 (Pak2) by transfection of Pak2-siRNA abolished high glucose-induced Syk activation. In summary, high glucose-induced TGF-{beta}1 gene transcription occurred through Pak2, Syk and subsequent ERK/AP-1 and NF-{kappa}B pathways. This suggests that Syk might be implicated in the diabetic kidney disease.

  5. Cytotoxicological Response to Engineered Nanomaterials: A Pathway-Driven Process

    E-Print Network [OSTI]

    Romoser, Amelia Antonia

    2012-07-16T23:59:59.000Z

    to deal with the ?insult? of the nanoparticle exposure by activating Nrf-2, a key transcription 10 factor involved in upregulating antioxidant genes, or the exposure may trigger more of an inflammatory response, in which NF-?B plays a role. A... disturbance of the cellular redox equilibrium and subsequent oxidative stress has been shown to trigger multiple stress pathways and transcription factors of redox-sensitive nature, such as nuclear factor-erythroid 2-p45?related factor 2 (Nrf2), which...

  6. Sheep Breeding and Feeding.

    E-Print Network [OSTI]

    Jones, J. M. (John McKinley)

    1917-01-01T23:59:59.000Z

    L. J. HART, Chairman WILL A. MILLER, JR. GOVERNING BOARD, STATE SUBSTATIONS ....................................... P L DOWNS President Temple.. .Term expires 1919 ................................. c~ARLE~ ROAAN. vice-hrerid~nf. Austin. .Term..., Inspector T. H. WOLTERS. Inspecfor S. D. PEARCE, Inspector W. M. WICKES, Inspector SUBSTATION NO. 1: Beeville, Bee Count] E. E. BINFORD, B. S., Super~ntendenf SUBSTATION'NO. 2: Troup, Smith Count: W. S. HOTCHKISS, Superintendent SUBSTATION NO. 3...

  7. Design of a 465 MW Combined Cycle Cogeneration Plant

    E-Print Network [OSTI]

    Leffler, D. W.

    STEAM TUR8JNE GENERAIOR ELECTRICAl, POWER OUIPUI GAS TURBINE GENERAIORS ~==3:=:J PROCESS CONDENSATE TOIAl fUEl 90 MillION BBl./'l'R NEI ELECTRICAl GENERATION 46$.000 KW LOSSES Sl,\\OF JUHINPUI NfTHEAT . 10 PROCESS 43% EFFICIENT... energy efficiency within this operating envelope, the following design .features are incorporated: extraction-induction-condensing steam turbine modulating inlet guide vanes on the gas turbine~ supplementary firing on two boilers steam augmentation...

  8. Assessment of energetic costs of AhR activation by ?-naphthoflavone in rainbow trout (Oncorhynchus mykiss) hepatocytes using metabolic flux analysis

    SciTech Connect (OSTI)

    Nault, Rance, E-mail: naultran@msu.edu [Ottawa-Carleton Institute of Biology, Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, K1N 6N5 (Canada); Abdul-Fattah, Hiba [Ottawa-Carleton Institute of Biology, Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, K1N 6N5 (Canada); Mironov, Gleb G.; Berezovski, Maxim V. [Ottawa-Carleton Institute of Biology, Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, K1N 6N5 (Canada); Department of Chemistry, University of Ottawa, Ottawa, Ontario, K1N 6N5 (Canada); Moon, Thomas W. [Ottawa-Carleton Institute of Biology, Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, K1N 6N5 (Canada)

    2013-08-15T23:59:59.000Z

    Exposure to environmental contaminants such as activators of the aryl hydrocarbon receptor (AhR) leads to the induction of defense and detoxification mechanisms. While these mechanisms allow organisms to metabolize and excrete at least some of these environmental contaminants, it has been proposed that these mechanisms lead to significant energetic challenges. This study tests the hypothesis that activation of the AhR by the model agonist ?-naphthoflavone (?NF) results in increased energetic costs in rainbow trout (Oncorhynchus mykiss) hepatocytes. To address this hypothesis, we employed traditional biochemical approaches to examine energy allocation and metabolism including the adenylate energy charge (AEC), protein synthesis rates, Na{sup +}/K{sup +}-ATPase activity, and enzyme activities. Moreover, we have used for the first time in a fish cell preparation, metabolic flux analysis (MFA) an in silico approach for the estimation of intracellular metabolic fluxes. Exposure of trout hepatocytes to 1 ?M ?NF for 48 h did not alter hepatocyte AEC, protein synthesis, or Na{sup +}/K{sup +}-ATPase activity but did lead to sparing of glycogen reserves and changes in activities of alanine aminotransferase and citrate synthase suggesting altered metabolism. Conversely, MFA did not identify altered metabolic fluxes, although we do show that the dynamic metabolism of isolated trout hepatocytes poses a significant challenge for this type of approach which should be considered in future studies. - Highlights: Energetic costs of AhR activation by ?NF was examined in rainbow trout hepatocytes. Metabolic flux analysis was performed on a fish cell preparation for the first time. Exposure to ?NF led to sparing of glycogen reserves and altered enzyme activities. Adenylate energy charge was maintained despite temporal changes in metabolism.

  9. Newsfront 12-18 March 2007, Issue 7

    E-Print Network [OSTI]

    Ghimire, Yubaraj

    for environment protection, Surya Nepal Asha for community empowerment, Surya Nepal Suswasthya for community health, and Surya Nepal Khelparyatan for promoting sports and tourism. Norway proposes US disposes NF Correspondent The United States clearly disapproved... the Police Chief, that nothing 'that would hamper the peace process should be done', even if that meant turning a blind eye to the crimes; and the message was relayed down the police ranks. It is only but natural for criminals feel a bit safe during...

  10. A New Formula for Prostate Cancer Lymph Node Risk

    SciTech Connect (OSTI)

    Yu, James B., E-mail: james.b.yu@yale.ed [Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT (United States); Yale Cancer Center, New Haven, CT (United States); Makarov, Danil V. [Robert Wood Johnson Clinical Scholars Program, Yale School of Medicine, New Haven, CT (United States); Section of Urology, Yale School of Medicine, New Haven, CT (United States); Department of Veterans Affairs Connecticut Healthcare System, West Haven, CT (United States); Gross, Cary [Robert Wood Johnson Clinical Scholars Program, Yale School of Medicine, New Haven, CT (United States); Department of Internal Medicine, Yale School of Medicine, New Haven, CT (United States)

    2011-05-01T23:59:59.000Z

    Introduction: The successful treatment of prostate cancer depends on the accurate estimation of the risk of regional lymph node (LN) involvement. The Roach formula (RF) has been criticized as overestimating LN risk. A modification of the RF has been attempted by other investigators using simplified adjustment ratios: the Nguyen formula (NF). Methods and Materials: The National Cancer Institute Surveillance, Epidemiology, and End Results database was investigated for patients treated in 2004 through 2006 for whom at least 10 LN were examined at radical prostatectomy, cT1c or cT2 disease, and prostate-specific antigen (PSA) <26 ng/ml (N = 2,930). The Yale formula (YF) was derived from half of the sample (n = 1,460), and validated in the other half (n = 1,470). Results: We identified 2,930 patients. Only 4.6% of patients had LN+, and 72.6% had cT1c disease. Gleason (GS) 8-10 histology was found in 14.4% of patients. The YF for prediction of %LN+ risk is [GS - 5]x [PSA/3 + 1.5 x T], where T = 0, 1, and 2 for cT1c, cT2a, and cT2b/cT2c. Within each strata of predicted %LN+ risk, the actual %LN+ was closest to the YF. Using a >15% risk as an indicator of high-risk disease, the YF had increased sensitivity (39.0% vs. 13.6%) compared with the NF, without a significant reduction in specificity (94.9% vs. 98.8%). The NF was overly restrictive of the high-risk group, with only 2% of patients having a >15% risk of LN+ by that formula. Conclusion: The YF performed better than the RF and NF and was best at differentiating patients at high risk for LN+ disease.

  11. The Texas Cotton Association and the AAA

    E-Print Network [OSTI]

    Chapman, David L

    1973-01-01T23:59:59.000Z

    :o, , oi a ' 4" t i "a~ i, ssssts nf e:: . f" r y!o a ' rg t;, w, soe iig, i o" a' ' ~ aa ~ - nr o isi sg" ay s s 'rgiii. it& Miler earaiaiiaao~:, . 'ii lie''& o ~ ' s "ski i &atarasto ~ . ' root-ra a s ' ~vi1 rel ao eoeal re ":s;g, toe ra:fz&s'ltsa f...

  12. Evaluation of high- and low-protein sorghum grains supplemented with lysine and lysine plus threonine as a feed for finishing swine

    E-Print Network [OSTI]

    Kinslow, William Freddie

    1972-01-01T23:59:59.000Z

    /or methionine content are below the finishing pigs' requirements for these amino acids. Sorghum grain supplemented with these amino acids, either singularly or in combination, plus needed vitamins and minerals should theoretically provide optimum growth..., there was no significant difference in retained nitrogen. The nutritive value of two sorghum grains containing 7. 9 and 11. 8% protein was compared on the basis of rat growth and amino acid analyses by Waggle eX nf. (1966). The high protein sorghum grain had higher...

  13. Fertilizers for Rice in Texas.

    E-Print Network [OSTI]

    Reynolds, E. B. (Elbert Brunner); Wyche, R. H. (Robert Henry)

    1929-01-01T23:59:59.000Z

    LIBRARY, CAMPUS. TEXAS AGRICULTURAL EXPERIMENT STATION 1 BULLETIN NO. 398 , DIVISION OF AGRONOMY FERTILIZERS FOR RICE IN TEXAS GRICULTURAL AND MECHANICAL COLLEGE OF TEXAS T. 0. \\\\TALTON, President STATION DMINISTRATION: A. B. CONNER, M. S... of Agriculture. ***Tm n,,nn-n+;,n "4th thm Cphnnl nf Aon~l~lt~lrp Experiments with fertilizers on rice at Substation No. 4, Texas Agricultural Experiment Station, Beaumont, Texas, show that the soils responded to nitrogen and phosphoric acid, but nitrogen...

  14. Results on the disconnected contributions for hadron structure

    E-Print Network [OSTI]

    Constantia Alexandrou; Martha Constantinou; Vincent Drach; Kyriakos Hadjiyiannakou; Karl Jansen; Giannis Koutsou; Alejandro Vaquero

    2014-12-19T23:59:59.000Z

    We present results on the disconnected contributions to three point functions entering in studies of hadron structure. We use $N_F = 2+1+1$ twisted mass fermions and give a detailed description on the results of the nucleon {\\sigma}-terms, isoscalar axial charge and first moments of bare parton distributions for a range of pions masses. In addition we give the {\\sigma}-terms and the computations are performed using QUDA code implemented on GPUs.

  15. Four-nucleon force in chiral effective field theory

    SciTech Connect (OSTI)

    Evgeny Epelbaum

    2005-10-25T23:59:59.000Z

    We derive the leading contribution to the four--nucleon force within the framework of chiral effective field theory. It is governed by the exchange of pions and the lowest--order nucleon--nucleon contact interaction and includes effects due to the nonlinear pion--nucleon couplings and the pion self interactions constrained by the chiral symmetry of QCD. The resulting 4NF does not contain any unknown parameters and can be tested in future few--and many--nucleon studies.

  16. The trees, shrubs, vines and ground covers of Austin, Texas: a study of the cultivated plant materials in a central Texas landscape

    E-Print Network [OSTI]

    Cole, Deborah Elaine

    1976-01-01T23:59:59.000Z

    of new or existing nursery sites might be evaluated for f'ield or container grown plants by referring to edaphic or climatological data. Landscape planners and park managers might utilize the data obtained to develop more diverse, more stable... Physiograohy nf the Study Area The late B. C. Tharp, botanist, once remarked, "Austin occupies a i7ost strategic position in being situated on a line where eastern and western floras meet" (96). while this statement refers to. natural vegetation...

  17. The nature and formation of coke in the reaction of methanol to hydrocarbons over chabazite

    E-Print Network [OSTI]

    McLaughlin, Kenneth Woot

    1983-01-01T23:59:59.000Z

    45 Figure 9. Relationship between enhanced coke formation with increasing yields of paraffins and diminishing yields of olefins 46 Figure 10. Gas chromatogram of the concentrated carbon tetrachloride extract of spent catalyst pellets . . . 49...THE NATURE ABD FORMATION OF COKE IB THE REACTIOB OF METHANOL TO HIDROCARBOBS OVER CHABAZITE A Thesis KENNETH WOOT MCLAUGHLLN Submitted to the Graduate College of Texas AAM Univers ty partial. fulfillment nf the req~nt fo~he degree of MASTER...

  18. Embeddings of Surfaces, Curves, and Moving Points in Euclidean Space

    E-Print Network [OSTI]

    Agarwal, Pankaj K.

    -86013, EIA-01-31905, CCR-02-04118, and DEB-04-25465, by an ARO grant W911NF-04-1-0278, and by a grant from, BM01]. See also the book [Vem04] and the surveys [Lin02, Ind01, IM04]. It versatility raises applications, such as video databases and complex motion databases (e.g., human motion or protein folding

  19. Design of a 465 MW Combined Cycle Cogeneration Plant

    E-Print Network [OSTI]

    Leffler, D. W.

    1986-01-01T23:59:59.000Z

    STEAM TUR8JNE GENERAIOR ELECTRICAl, POWER OUIPUI GAS TURBINE GENERAIORS ~==3:=:J PROCESS CONDENSATE TOIAl fUEl 90 MillION BBl./'l'R NEI ELECTRICAl GENERATION 46$.000 KW LOSSES Sl,\\OF JUHINPUI NfTHEAT . 10 PROCESS 43% EFFICIENT... energy efficiency within this operating envelope, the following design .features are incorporated: extraction-induction-condensing steam turbine modulating inlet guide vanes on the gas turbine~ supplementary firing on two boilers steam augmentation...

  20. ENEA-UCI Structures of the low frequency Alfven continuous spectrum 1 Structures of the low frequency Alfven

    E-Print Network [OSTI]

    Zonca, Fulvio

    (KTI) gap (L. Chen 2007, NF 47 S727) Diamagnetic drift: KBM (H. Biglari, et al. 1991, PRL 67 3681) Thermal ion compress.: BAE (W.W. Heidbrink, et al. 1993, PRL 71 855) Ti and wave-part. resonances: AITG Alfv´en continuous spectrum 5 R.Nazikian,etal.06,PRL96,105006 R. Nazikian, et al. 06, PRL 96, 105006

  1. miR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohns disease

    SciTech Connect (OSTI)

    Chen, Yu; Wang, Chengxiao; Liu, Ying; Tang, Liwei; Zheng, Mingxia [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China); Xu, Chundi [Department of Pediatrics, Ruijin affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200025 (China)] [Department of Pediatrics, Ruijin affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, 200025 (China); Song, Jian, E-mail: jiansongkxy@126.com [Department of Gastroenterology, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Gastroenterology, Jiangwan Hospital of Shanghai, Shanghai 200434 (China); Meng, Xiaochun [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)] [Department of Pediatrics, Jiangwan Hospital of Shanghai, Shanghai 200434 (China)

    2013-08-16T23:59:59.000Z

    Highlights: NOD2 is a target gene of miR-122. miR-122 inhibits LPS-induced apoptosis by suppressing NOD2 in HT-29 cells. miR-122 reduces the expression of pro-inflammatory cytokines (TNF-? and IFN-?). miR-122 promotes the release of anti-inflammatory cytokines (IL-4 and IL-10). NF-?B signaling pathway is involved in inflammatory response induced by LPS. -- Abstract: Crohns disease (CD) is one of the two major types of inflammatory bowel disease (IBD) thought to be caused by genetic and environmental factors. Recently, miR-122 was found to be deregulated in association with CD progression. However, the underlying molecular mechanisms remain unclear. In the present study, the gene nucleotide-binding oligomerization domain 2 (NOD2/CARD15), which is strongly associated with susceptibility to CD, was identified as a functional target of miR-122. MiR-122 inhibited LPS-induced apoptosis by suppressing NOD2 in HT-29 cells. NOD2 interaction with LPS initiates signal transduction mechanisms resulting in the activation of nuclear factor ?B (NF-?B) and the stimulation of downstream pro-inflammatory events. The activation of NF-?B was inhibited in LPS-stimulated HT-29 cells pretreated with miR-122 precursor or NOD2 shRNA. The expression of the pro-inflammatory cytokines TNF-? and IFN-? was significantly decreased, whereas therelease of the anti-inflammatory cytokines IL-4 and IL-10 was increased in LPS-stimulated HT-29 cells pretreated with miR-122 precursor, NOD2 shRNA or the NF-?B inhibitor QNZ. Taken together, these results indicate that miR-122 and its target gene NOD2 may play an important role in the injury of intestinal epithelial cells induced by LPS.

  2. A study of the point reactor dynamics equations as applied to large nuclear excursions

    E-Print Network [OSTI]

    Perry, Robert Terrell

    1967-01-01T23:59:59.000Z

    =I, NF IF( J-4) 67, 67, 68 FIET. D(1, JW) =F( T) FA ( J) =F ( J) SF=0. O DO 76 I=1, ND SF=SF+FR(I) '4WA(I) OA=(B/EI. L*((RA-1. 0)*ENA+SF+S))/ENA FIET. D(1, 4) =OA H=HI H2=H/2. 0 NPC=1 KKL = DD(376) KKK = DD(374) CONTINUE CAT I. FELBCK NPC...

  3. Evaluation of six cycles of reciprocal recurrent selection in maize

    E-Print Network [OSTI]

    Llorente, Carlos Fernando

    1970-01-01T23:59:59.000Z

    . The progress obtained was measured by the yield nf selections x composites and frequency di. stributions of higher yieldin- elec- tions. Variability was estimated by the standard deviatson, co- efficient of variation, and range of the . elections... ? composites yield trials. Coefficients of inbreeding were also calculated for each composite at th. . beginning of every cycle, Results indicated t?. at reciprocal recurrent selection improved the frequency of higher yielding selections in the Ferguson...

  4. Newsfront 10-16 March 2008, Issue 57

    E-Print Network [OSTI]

    Ghimire, Yubaraj

    of Shivaratri. Two mistakes nf correspondent With just a month left for the polls to the constituent assembly, Prime Minister GP Koirala is giving conflicting messages regarding the party unity and the citizenship issue. According to senior leaders... Koirala and nephew Shekhar Koirala. PM Koiralas request was turned down by MJF leader Upendra Yadav and Bijay Gachchedar who are contest- ing election to the constituent assembly from Sunsari and Morang against Sujata and Shekhar respectively. PM Koirala...

  5. Anti-inflammatory effect of resveratrol on TNF-{alpha}-induced MCP-1 expression in adipocytes

    SciTech Connect (OSTI)

    Zhu Jian [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029 (China); Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Science, Jiangsu Province Diabetes Center, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029 (China); Yong Wei [Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Science, Jiangsu Province Diabetes Center, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029 (China); Wu Xiaohong [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029 (China); Yu Ying; Lv Jinghuan [Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Science, Jiangsu Province Diabetes Center, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029 (China); Liu Cuiping; Mao Xiaodong [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029 (China); Zhu Yunxia [Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Science, Jiangsu Province Diabetes Center, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029 (China); Xu Kuanfeng [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029 (China); Han Xiao [Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Science, Jiangsu Province Diabetes Center, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029 (China)], E-mail: hanxiao@njmu.edu.cn; Liu Chao [Department of Endocrinology, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029 (China)], E-mail: liuchao@nfmcn.com

    2008-05-02T23:59:59.000Z

    Chronic low-grade inflammation characterized by adipose tissue macrophage accumulation and abnormal cytokine production is a key feature of obesity and type 2 diabetes. Adipose-tissue-derived monocyte chemoattractant protein (MCP)-1, induced by cytokines, has been shown to play an essential role in the early events during macrophage infiltration into adipose tissue. In this study we investigated the effects of resveratrol upon both tumor necrosis factor (TNF)-{alpha}-induced MCP-1 gene expression and its underlying signaling pathways in 3T3-L1 adipoctyes. Resveratrol was found to inhibit TNF-{alpha}-induced MCP-1 secretion and gene transcription, as well as promoter activity, which based on down-regulation of TNF-{alpha}-induced MCP-1 transcription. Nuclear factor (NF)-{kappa}B was determined to play a major role in the TNF-{alpha}-induced MCP-1 expression. Further analysis showed that resveratrol inhibited DNA binding activity of the NF-{kappa}B complex and subsequently suppressed NF-{kappa}B transcriptional activity in TNF-{alpha}-stimulated cells. Finally, the inhibition of MCP-1 may represent a novel mechanism of resveratrol in preventing obesity-related pathologies.

  6. Achieving very low mercury levels in refinery wastewater by membrane filtration.

    SciTech Connect (OSTI)

    Urgun Demirtas, M.; Benda, P.; Gillenwater, P. S.; Negri, M. C.; Xiong, H.; Snyder, S. W. (Center for Nanoscale Materials); ( ES)

    2012-05-15T23:59:59.000Z

    Microfiltration (MF), ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO) membranes were evaluated for their ability to achieve the world's most stringent Hg discharge criterion (<1.3 ng/L) in an oil refinery's wastewater. The membrane processes were operated at three different pressures to demonstrate the potential for each membrane technology to achieve the targeted effluent mercury concentrations. The presence of mercury in the particulate form in the refinery wastewater makes the use of MF and UF membrane technologies more attractive in achieving very low mercury levels in the treated wastewater. Both NF and RO were also able to meet the target mercury concentration at lower operating pressures (20.7 bar). However, higher operating pressures ({ge}34.5 bar) had a significant effect on NF and RO flux and fouling rates, as well as on permeate quality. SEM images of the membranes showed that pore blockage and narrowing were the dominant fouling mechanisms for the MF membrane while surface coverage was the dominant fouling mechanism for the other membranes. The correlation between mercury concentration and particle size distribution was also investigated to understand mercury removal mechanisms by membrane filtration. The mean particle diameter decreased with filtration from 1.1 {+-} 0.0 {micro}m to 0.74 {+-} 0.2 {micro}m after UF.

  7. Thymosin beta-4 promotes mesenchymal stem cell proliferation via an interleukin-8-dependent mechanism

    SciTech Connect (OSTI)

    Jeon, Byung-Joon [Department of Plastic and Reconstructive Surgery, Korea University Medical Center, Gojan 1-dong, Danwon-gu, Ansan-si, Gyeonggi-do 425-707 (Korea, Republic of); Yang, Yoolhee; Kyung Shim, Su [Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710 (Korea, Republic of); Yang, Heung-Mo [Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710 (Korea, Republic of); Cho, Daeho, E-mail: cdhkor@sookmyung.ac.kr [Department of Life Science, Sookmyung Women's University, Hyochangwon-gil 52, Yongsan-gu, Seoul 140-742 (Korea, Republic of); Ik Bang, Sa, E-mail: si55.bang@samsung.com [Department of Plastic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710 (Korea, Republic of)

    2013-10-15T23:59:59.000Z

    Mesenchymal stem cells (MSCs) hold great promise for the field of tissue regeneration. Because only a limited number of MSCs can be obtained from each donor site, it is important to establish standard methods for MSC expansion using growth and trophic factors. Thymosin ?4 (T?4) is a novel trophic factor that has antimicrobial effects and the potential to promote tissue repair. T?4 is a ubiquitous, naturally-occurring peptide in the wound bed. Therefore, the relationship between T?4 and MSCs, especially adjacent adipose tissue-derived stem cells (ASCs), merits consideration. Exogenous T?4 treatment enhanced the proliferation of human ASCs, resulting in prominent nuclear localization of PCNA immunoreactivity. In addition, exogenous T?4 also increased IL-8 secretion and blocking of IL-8 with neutralizing antibodies decreased T?4-induced ASC proliferation, suggesting that IL-8 is a critical mediator of T?4-enhanced proliferation. Moreover, T?4 activated phosphorylation of ERK1/2 and increased the nuclear translocation of NF-?B. These observation provide that T?4 promotes the expansion of human ASCs via an IL-8-dependent mechanism that involves the ERK and NF-?B pathways. Therefore, T?4 could be used as a tool for MSC expansion in cell therapeutics. - Highlights: This is fundamental information required to correlate T?4 with MSC expansion. MSC expansion by T?4 is involved in enhancement of IL-8 and ERK/NF-?B pathway. T?4 could be used as a tool for MSC expansion in cell therapeutics.

  8. Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice

    SciTech Connect (OSTI)

    Li, Weifeng, E-mail: liwf@mail.xjtu.edu.cn; Huang, Huimin; Niu, Xiaofeng, E-mail: niuxf@mail.xjtu.edu.cn; Fan, Ting; Mu, Qingli; Li, Huani

    2013-10-01T23:59:59.000Z

    Excessive alcohol consumption can lead to gastric ulcer and the present work was aimed to examine the protective effect of tetrahydrocoptisine (THC) in the model of ethanol-induced gastric ulcer in mice. Fasted mice treated with ethanol 75% (0.5 ml/100 g) were pre-treated with THC (10 or 20 mg/kg, ip), cimetidine (100 mg/kg, ip) or saline in different experimental sets for a period of 3 days, and animals were euthanized 4 h after ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. The results showed that ethanol induced gastric damage, improving nitric oxide (NO) level, increased pro-inflammatory cytokine (TNF-? and IL-6) levels and myeloperoxidase (MPO) activity, as well as the expression of nuclear factor-?B (NF-?B) in the ethanol group. Pretreatment of THC at doses of 10 and 20 mg/kg bodyweight significantly attenuated the gastric lesions as compared to the ethanol group. These results suggest that the gastroprotective activity of THC is attributed to reducing NO production and adjusting the pro-inflammatory cytokine, inhibited neutrophil accumulation and NF-?B expression. - Highlights: THC decreased ethanol-induced pro-inflammatory cytokine release. THC inhibited the production of NO in serum and gastric tissue. THC reduced NF-?B expression and MPO accumulation in ethanol-induced gastric tissue.

  9. Requirement of T-lymphokine-activated killer cell-originated protein kinase for TRAIL resistance of human HeLa cervical cancer cells

    SciTech Connect (OSTI)

    Kwon, Hyeok-Ran [Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon 302-718 (Korea, Republic of)] [Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon 302-718 (Korea, Republic of); Lee, Ki Won [Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701 (Korea, Republic of)] [Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701 (Korea, Republic of); Dong, Zigang [Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912 (United States)] [Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912 (United States); Lee, Kyung Bok [Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon 302-718 (Korea, Republic of)] [Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon 302-718 (Korea, Republic of); Oh, Sang-Muk, E-mail: sangmuk_oh@konyang.ac.kr [Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon 302-718 (Korea, Republic of)] [Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon 302-718 (Korea, Republic of)

    2010-01-01T23:59:59.000Z

    T-lymphokine-activated killer cell-originated protein kinase (TOPK) appears to be highly expressed in various cancer cells and to play an important role in maintaining proliferation of cancer cells. However, the underlying mechanism by which TOPK regulates growth of cancer cells remains elusive. Here we report that upregulated endogenous TOPK augments resistance of cancer cells to apoptosis induced by tumor necrosis factor-related apoptosis inducing ligand (TRAIL). Stable knocking down of TOPK markedly increased TRAIL-mediated apoptosis of human HeLa cervical cancer cells, as compared with control cells. Caspase 8 or caspase 3 activities in response to TRAIL were greatly incremented in TOPK-depleted cells. Ablation of TOPK negatively regulated TRAIL-mediated NF-{kappa}B activity. Furthermore, expression of NF-{kappa}B-dependent genes, FLICE-inhibitory protein (FLIP), inhibitor of apoptosis protein 1 (c-IAP1), or X-linked inhibitor of apoptosis protein (XIAP) was reduced in TOPK-depleted cells. Collectively, these findings demonstrated that TOPK contributed to TRAIL resistance of cancer cells via NF-{kappa}B activity, suggesting that TOPK might be a potential molecular target for successful cancer therapy using TRAIL.

  10. Solvothermal synthesis of NiAl double hydroxide microspheres on a nickel foam-graphene as an electrode material for pseudo-capacitors

    SciTech Connect (OSTI)

    Momodu, Damilola; Bello, Abdulhakeem; Dangbegnon, Julien; Barzeger, Farshad; Taghizadeh, Fatimeh; Fabiane, Mopeli; Manyala, Ncholu, E-mail: ncholu.manyala@up.ac.za [Department of Physics, Institute of Applied Materials, SARChI Chair in Carbon Technology and Materials, University of Pretoria, Pretoria 0028, South Africa. (South Africa); Johnson, A. T. Charlie [Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104 (United States)

    2014-09-15T23:59:59.000Z

    In this paper, we demonstrate excellent pseudo-capacitance behavior of nickel-aluminum double hydroxide microspheres (NiAl DHM) synthesized by a facile solvothermal technique using tertbutanol as a structure-directing agent on nickel foam-graphene (NF-G) current collector as compared to use of nickel foam current collector alone. The structure and surface morphology were studied by X-ray diffraction analysis, Raman spectroscopy and scanning and transmission electron microscopies respectively. NF-G current collector was fabricated by chemical vapor deposition followed by an ex situ coating method of NiAl DHM active material which forms a composite electrode. The pseudocapacitive performance of the composite electrode was investigated by cyclic voltammetry, constant chargedischarge and electrochemical impedance spectroscopy measurements. The composite electrode with the NF-G current collector exhibits an enhanced electrochemical performance due to the presence of the conductive graphene layer on the nickel foam and gives a specific capacitance of 1252 F g{sup ?1} at a current density of 1 A g{sup ?1} and a capacitive retention of about 97% after 1000 chargedischarge cycles. This shows that these composites are promising electrode materials for energy storage devices.

  11. Systematic study of three-nucleon force effects in the cross section of the deuteron-proton breakup at 130 MeV

    E-Print Network [OSTI]

    St. Kistryn; E. Stephan; A. Biegun; K. Bodek; A. Deltuva; E. Epelbaum; K. Ermisch; W. Gloeckle; J. Golak; N. Kalantar-Nayestanaki; H. Kamada; M. Kis; B. Klos; A. Kozela; J. Kuros-Zolnierczuk; M. Mahjour-Shafiei; U. -G. Meissner; A. Micherdzinska; A. Nogga; P. U. Sauer; R. Skibinski; R. Sworst; H. Witala; J. Zejma; W. Zipper

    2005-08-11T23:59:59.000Z

    High precision cross-section data of the deuteron-proton breakup reaction at 130 MeV are presented for 72 kinematically complete configurations. The data cover a large region of the available phase space, divided into a systematic grid of kinematical variables. They are compared with theoretical predictions, in which the full dynamics of the three-nucleon (3N) system is obtained in three different ways: realistic nucleon-nucleon (NN) potentials are combined with model 3N forces (3NF's) or with an effective 3NF resulting from explicit treatment of the Delta-isobar excitation. Alternatively, the chiral perturbation theory approach is used at the next-to-next-to-leading order with all relevant NN and 3N contributions taken into account. The generated dynamics is then applied to calculate cross-section values by rigorous solution of the 3N Faddeev equations. The comparison of the calculated cross sections with the experimental data shows a clear prefernce for the predictions in which the 3NF's are included. The majority of the experimental data points is well reproduced by the theoretical predictions. The remaining discrepancies are investigated by inspecting cross sections integrated over certain kinematical variables. The procedure of global comparisons leads to establishing regularities in disagreements between the experimental data and the theoretically predicted values of the cross sections. They indicate deficiencies still present in the assumed models of the 3N system dynamics.

  12. Macrophage-stimulating protein attenuates gentamicin-induced inflammation and apoptosis in human renal proximal tubular epithelial cells

    SciTech Connect (OSTI)

    Lee, Ko Eun [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Eun Young [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Chang Seong; Choi, Joon Seok; Bae, Eun Hui; Ma, Seong Kwon [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Kyung Keun [Department of Pharmacology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Pharmacology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Lee, Jong Un [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Soo Wan, E-mail: skimw@chonnam.ac.kr [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)

    2013-05-10T23:59:59.000Z

    Highlights: MSP/RON system is activated in rat kidney damaged by gentamicin. MSP inhibits GM-induced cellular apoptosis and inflammation in HK-2 cells. MSP attenuates GM-induced activation of MAPKs and NF-?B pathways in HK-2 cells. -- Abstract: The present study aimed to investigate whether macrophage-stimulating protein (MSP) treatment attenuates renal apoptosis and inflammation in gentamicin (GM)-induced tubule injury and its underlying molecular mechanisms. To examine changes in MSP and its receptor, recepteur dorigine nantais (RON) in GM-induced nephropathy, rats were injected with GM for 7 days. Human renal proximal tubular epithelial (HK-2) cells were incubated with GM for 24 h in the presence of different concentrations of MSP and cell viability was measured by MTT assay. Apoptosis was determined by flow cytometry of cells stained with fluorescein isothiocyanate-conjugated annexin V protein and propidium iodide. Expression of Bcl-2, Bax, caspase-3, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-?B), I?B-?, and mitogen-activated protein kinases (MAPKs) was analyzed by semiquantitative immunoblotting. MSP and RON expression was significantly greater in GM-treated rats, than in untreated controls. GM-treatment reduced HK-2 cell viability, an effect that was counteracted by MSP. Flow cytometry and DAPI staining revealed GM-induced apoptosis was prevented by MSP. GM reduced expression of anti-apoptotic protein Bcl-2 and induced expression of Bax and cleaved caspase 3; these effects and GM-induced expression of COX-2 and iNOS were also attenuated by MSP. GM caused MSP-reversible induction of phospho-ERK, phospho-JNK, and phospho-p38. GM induced NF-?B activation and degradation of I?B-?; the increase in nuclear NF-?B was blocked by inhibitors of ERK, JNK, p-38, or MSP pretreatment. These findings suggest that MSP attenuates GM-induced inflammation and apoptosis by inhibition of the MAPKs/NF-?B signaling pathways.

  13. Functional Toll-like receptor 4 expressed in lactotrophs mediates LPS-induced proliferation in experimental pituitary hyperplasia

    SciTech Connect (OSTI)

    Sabatino, Mara Eugenia; Sosa, Liliana del Valle; Petiti, Juan Pablo; Mukdsi, Jorge Humberto [Centro de Microscopa Electrnica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Mdicas, Universidad Nacional de Crdoba, Av. Enrique Barros y Enfermera Gordillo, Ciudad Universitaria, CP 5000, Crdoba (Argentina); Mascanfroni, Ivn Daro; Pellizas, Claudia Gabriela [Centro de Investigaciones en Bioqumica Clnica e Inmunologa (CIBICI-CONICET), Facultad de Ciencias Qumicas, Universidad Nacional de Crdoba, Av. Haya de la Torre y Medina Allende, Ciudad Universitaria, CP 5000, Crdoba (Argentina); Gutirrez, Silvina; Torres, Alicia Ins [Centro de Microscopa Electrnica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Mdicas, Universidad Nacional de Crdoba, Av. Enrique Barros y Enfermera Gordillo, Ciudad Universitaria, CP 5000, Crdoba (Argentina); De Paul, Ana Luca, E-mail: adepaul@cmefcm.uncor.edu [Centro de Microscopa Electrnica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Mdicas, Universidad Nacional de Crdoba, Av. Enrique Barros y Enfermera Gordillo, Ciudad Universitaria, CP 5000, Crdoba (Argentina)

    2013-11-15T23:59:59.000Z

    Toll like receptor 4 (TLR4) has been characterized for its ability to recognize bacterial endotoxin lipopolysaccharide (LPS). Considering that infections or inflammatory processes might contribute to the progression of pituitary tumors, we analyzed the TLR4 functional role by evaluating the LPS effect on lactotroph proliferation in primary cultures from experimental pituitary tumors, and examined the involvement of PI3K-Akt and NF-?B activation in this effect. In addition, the role of 17?-estradiol as a possible modulator of LPS-induced PRL cell proliferation was further investigated. In estrogen-induced hyperplasic pituitaries, LPS triggered lactotroph cell proliferation. However, endotoxin failed to increase the number of lactotrophs taking up BrdU in normal pituitaries. Moreover, incubation with anti-TLR4 antibody significantly reduced LPS-induced lactotroph proliferation, suggesting a functional role of this receptor. As a sign of TLR4 activation, an LPS challenge increased IL-6 release in normal and tumoral cells. By flow cytometry, TLR4 baseline expression was revealed at the plasma membrane of tumoral lactotrophs, without changes noted in the percentage of double PRL/TLR4 positive cells after LPS stimulus. Increases in TLR4 intracellular expression were detected as well as rises in CD14, p-Akt and NF-?B after an LPS challenge, as assessed by western blotting. The TLR4/PRL and PRL/NF-?B co-localization was also corroborated by immunofluorescence and the involvement of PI3K/Akt signaling in lactotroph proliferation and IL-6 release was revealed through the PI3K inhibitor Ly-294002. In addition, 17?-estradiol attenuated the LPS-evoked increase in tumoral lactotroph proliferation and IL-6 release. Collectively these results demonstrate the presence of functional TLR4 in lactotrophs from estrogen-induced hyperplasic pituitaries, which responded to the proliferative stimulation and IL-6 release induced by LPS through TLR4/CD14, with a contribution of the PI3K-Akt and NF-?B signaling pathways. - Highlights: In hyperplastic pituitaries, LPS triggered the lactotroph cell proliferation and IL-6 release. Functional Toll-like receptor 4 (TLR4) is expressed at the plasma membrane of tumoral lactotrophs. Increases in TLR4 and CD14 intracellular expression levels were detected after an LPS challenge. The proliferative stimulation and IL-6 release involved the PI3K-Akt pathway and NF-?B activation. 17?-estradiol attenuated the LPS-evoked tumoral lactotroph proliferation and IL-6 secretion.

  14. Green tea polyphenol epigallocatechin-3-gallate suppresses melanoma growth by inhibiting inflammasome and IL-1{beta} secretion

    SciTech Connect (OSTI)

    Ellis, Lixia Z.; Liu, Weimin; Luo, Yuchun; Okamoto, Miyako; Qu, Dovina; Dunn, Jeffrey H. [Department of Dermatology, University of Colorado School of Medicine, Aurora, CO 80045 (United States)] [Department of Dermatology, University of Colorado School of Medicine, Aurora, CO 80045 (United States); Fujita, Mayumi, E-mail: mayumi.fujita@ucdenver.edu [Department of Dermatology, University of Colorado School of Medicine, Aurora, CO 80045 (United States) [Department of Dermatology, University of Colorado School of Medicine, Aurora, CO 80045 (United States); Denver Veterans Affairs Medical Center, Denver, CO 80220 (United States)

    2011-10-28T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer EGCG inhibits melanoma cell growth at physiological doses (0.1-1 {mu}M). Black-Right-Pointing-Pointer EGCG inhibits melanoma cell growth via inflammasomes and IL-1{beta} suppression. Black-Right-Pointing-Pointer Inflammasomes and IL-1{beta} could be potential targets for future melanoma therapeutics. -- Abstract: Epigallocatechin-3-gallate (EGCG), the major polyphenolic component of green tea, has been demonstrated to possess anti-inflammatory, antioxidant, anti-mutagenic and anti-carcinogenic properties. The anti-melanoma effect of EGCG has been previously suggested, but no clear mechanism of action has been established. In this study, we demonstrated that EGCG inhibits melanoma cell growth at physiological doses (0.1-1 {mu}M). In the search for mechanisms of EGCG-mediated melanoma cell suppression, we found that NF-{kappa}B was inhibited, and that reduced NF-{kappa}B activity was associated with decreased IL-1{beta} secretion from melanoma cells. Since inflammasomes are involved in IL-1{beta} secretion, we investigated whether IL-1{beta} suppression was mediated by inflammasomes, and found that EGCG treatment led to downregulation of the inflammasome component, NLRP1, and reduced caspase-1 activation. Furthermore, silencing the expression of NLRP1 abolished EGCG-induced inhibition of tumor cell proliferation both in vitro and in vivo, suggesting a key role of inflammasomes in EGCG efficacy. This paper provides a novel mechanism for EGCG-induced melanoma inhibition: inflammasome downregulation {yields} decreased IL-1{beta} secretion {yields} decreased NF-{kappa}B activities {yields} decreased cell growth. In addition, it suggests inflammasomes and IL-1{beta} could be potential targets for future melanoma therapeutics.

  15. Uptake and cytotoxic effects of multi-walled carbon nanotubes in human bronchial epithelial cells

    SciTech Connect (OSTI)

    Hirano, Seishiro, E-mail: seishiro@nies.go.j [Environmental Nanotoxicology Section, RCER, National Institute for Environmental Studies (Japan); Fujitani, Yuji; Furuyama, Akiko [Environmental Nanotoxicology Section, RCER, National Institute for Environmental Studies (Japan); Kanno, Sanae [Photon Medical Research Center, Hamamatsu University School of Medicine (Japan)

    2010-11-15T23:59:59.000Z

    Carbon nanotubes (CNT) are cytotoxic to several cell types. However, the mechanism of CNT toxicity has not been fully studied, and dosimetric analyses of CNT in the cell culture system are lacking. Here, we describe a novel, high throughput method to measure cellular uptake of CNT using turbimetry. BEAS-2B, a human bronchial epithelial cell line, was used to investigate cellular uptake, cytotoxicity, and inflammatory effects of multi-walled CNT (MWCNT). The cytotoxicity of MWCNT was higher than that of crocidolite asbestos in BEAS-2B cells. The IC{sub 50} of MWCNT was 12 {mu}g/ml, whereas that of asbestos (crocidolite) was 678 {mu}g/ml. Over the course of 5 to 8 h, BEAS-2B cells took up 17-18% of the MWCNT when they were added to the culture medium at a concentration of 10 {mu}g/ml. BEAS-2B cells were exposed to 2, 5, or 10 {mu}g/ml of MWCNT, and total RNA was extracted for cytokine cDNA primer array assays. The culture supernatant was collected for cytokine antibody array assays. Cytokines IL-6 and IL-8 increased in a dose dependent manner at both the mRNA and protein levels. Migration inhibitory factor (MIF) also increased in the culture supernatant in response to MWCNT. A phosphokinase array study using lysates from BEAS-2B cells exposed to MWCNT indicated that phosphorylation of p38, ERK1, and HSP27 increased significantly in response to MWCNT. Results from a reporter gene assays using the NF-{kappa}B or AP-1 promoter linked to the luciferase gene in transiently transfected CHO-KI cells revealed that NF-{kappa}B was activated following MWCNT exposure, while AP-1 was not changed. Collectively, MWCNT activated NF-{kappa}B, enhanced phosphorylation of MAP kinase pathway components, and increased production of proinflammatory cytokines in human bronchial epithelial cells.

  16. Molecular mechanism of trichloroethylene-induced hepatotoxicity mediated by CYP2E1

    SciTech Connect (OSTI)

    Ramdhan, Doni Hikmat; Kamijima, Michihiro; Yamada, Naoyasu; Ito, Yuki; Yanagiba, Yukie; Nakamura, Daichi; Okamura, Ai; Ichihara, Gaku [Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine (Japan); Aoyama, Toshifumi [Department of Metabolic Regulation, Institute of Aging and Adaptation, Shinshu University Graduate School of Medicine (Japan); Gonzalez, Frank J. [Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, MD (United States); Nakajima, Tamie [Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine (Japan)], E-mail: tnasu23@med.nagoya-u.ac.jp

    2008-09-15T23:59:59.000Z

    Cytochrome P450 (CYP) 2E1 was suggested to be the major enzyme involved in trichloroethylene (TRI) metabolism and TRI-induced hepatotoxicity, although the latter molecular mechanism is not fully understood. The involvement of CYP2E1 in TRI-induced hepatotoxicity and its underlying molecular mechanism were studied by comparing hepatotoxicity in cyp2e1{sup +/+} and cyp2e1{sup -/-} mice. The mice were exposed by inhalation to 0 (control), 1000, or 2000 ppm of TRI for 8 h a day, for 7 days, and TRI-hepatotoxicity was assessed by measuring plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and histopathology. Urinary metabolites of trichloroethanol and trichloroacetic acid (TCA) were considerably greater in cyp2e1{sup +/+} compared to cyp2e1{sup -/-} mice, suggesting that CYP2E1 is the major P450 involved in the formation of these metabolites. Consistent with elevated plasma ALT and AST activities, cyp2e1{sup +/+} mice in the 2000 ppm group showed histopathological inflammation. TRI significantly upregulated PPAR{alpha}, which might function to inhibit NF{kappa}B p50 and p65 signalling. In addition, TRI-induced NF{kappa}B p52 mRNA, and significantly positive correlation between NF{kappa}B p52 mRNA expression and plasma ALT activity levels were observed, suggesting the involvement of p52 in liver inflammation. Taken together, the current study directly demonstrates that CYP2E1 was the major P450 involved in the first step of the TRI metabolism, and the metabolites produced may have two opposing roles: one inducing hepatotoxicity and the other protecting against the toxicity. Intermediate metabolite(s) from TRI to chloral hydrate produced by CYP2E1-mediated oxidation may be involved in the former, and TCA in the latter.

  17. Insights antifibrotic mechanism of methyl palmitate: Impact on nuclear factor kappa B and proinflammatory cytokines

    SciTech Connect (OSTI)

    Mantawy, Eman M.; Tadros, Mariane G. [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt)] [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt); Awad, Azza S. [Department of Pharmacology and Toxicology, Faulty of Pharmacy, Al-Azhar University, Cairo (Egypt)] [Department of Pharmacology and Toxicology, Faulty of Pharmacy, Al-Azhar University, Cairo (Egypt); Hassan, Dina A.A. [Department of Histology, Faculty of Medicine, Al-Azhar University, Cairo (Egypt)] [Department of Histology, Faculty of Medicine, Al-Azhar University, Cairo (Egypt); El-Demerdash, Ebtehal, E-mail: ebtehal_dm@yahoo.com [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt)] [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt)

    2012-01-01T23:59:59.000Z

    Fibrosis accompanies most chronic liver disorders and is a major factor contributing to hepatic failure. Therefore, the need for an effective treatment is evident. The present study was designed to assess the potential antifibrotic effect of MP and whether MP can attenuate the severity of oxidative stress and inflammatory response in chronic liver injury. Male albino rats were treated with either CCl{sub 4} (1 ml/kg, twice a week) and/or MP (300 mg/kg, three times a week) for six weeks. CCl{sub 4}-intoxication significantly increased liver weight, serum aminotransferases, total cholesterol and triglycerides while decreased albumin level and these effects were prevented by co-treatment with MP. As indicators of oxidative stress, CCl{sub 4}-intoxication caused significant glutathione depletion and lipid peroxidation while MP co-treatment preserved them within normal values. As markers of fibrosis, hydroxyproline content and ?-SMA expression increased markedly in the CCl{sub 4} group and MP prevented these alterations. Histopathological examination by both light and electron microscope further confirmed the protective efficacy of MP. To elucidate the antifibrotic mechanisms of MP, the expression of NF-?B, iNOS and COX-2 and the tissue levels of TNF-? and nitric oxide were assessed; CCl{sub 4} increased the expression of NF-?B and all downstream inflammatory cascade while MP co-treatment inhibited them. Collectively these findings indicate that MP possesses a potent antifibrotic effect which may be partly a consequence of its antioxidant and anti-inflammatory properties. -- Highlights: ? Methyl palmitate is free fatty acid methyl ester. ? It possesses a strong antifibrotic effect. ? It inhibits NF-?B and the consequent proinflammatory and oxidative stress response.

  18. Role of endoplasmic reticulum stress in acrolein-induced endothelial activation

    SciTech Connect (OSTI)

    Haberzettl, Petra; Vladykovskaya, Elena; Srivastava, Sanjay [Institute of Molecular Cardiology, Department of Medicine, University of Louisville, Louisville, KY 40202 (United States); Bhatnagar, Aruni [Institute of Molecular Cardiology, Department of Medicine, University of Louisville, Louisville, KY 40202 (United States)], E-mail: aruni@louisville.edu

    2009-01-01T23:59:59.000Z

    Acrolein is a ubiquitous environmental pollutant and an endogenous product of lipid peroxidation. It is also generated during the metabolism of several drugs and amino acids. In this study, we examined the effects of acrolein on endothelial cells. Treatment of human umbilical vein endothelial cells (HUVECs) with 2 to 10 {mu}M acrolein led to an increase in the phosphorylation of eIF-2{alpha} within 10 to 30 min of exposure. This was followed by alternate splicing of XBP-1 mRNA and an increase in the expression of the endoplasmic reticulum (ER) chaperone genes Grp78 and Herp. Within 2-4 h of treatment, acrolein also increased the abundance and the nuclear transport of the transcription factors ATF3, AFT4, and CHOP. Acrolein-induced increase in ATF3 was prevented by treating the cells with the chemical chaperone - phenylbutyric acid (PBA). Treatment with acrolein increased phosphorylation of ERK1/2, p38, and JNK. The increase in JNK phosphorylation was prevented by PBA. Acrolein treatment led to activation and nuclear translocation of the transcription factor NF-{kappa}B and an increase in TNF-{alpha}, IL-6 and IL-8, but not MCP-1, mRNA. Increased expression of cytokine genes and NF-{kappa}B activation were not observed in cells treated with PBA. These findings suggest that exposure to acrolein induces ER stress and triggers the unfolded protein response and that NF-{kappa}B activation and stimulation of cytokine production by acrolein could be attributed, in part, to ER stress. Chemical chaperones of protein-folding may be useful in treating toxicological and pathological states associated with excessive acrolein exposure or production.

  19. Climax vegetation and soils of the Blackland Prairie of Texas

    E-Print Network [OSTI]

    Collins, Odell Brown

    1972-01-01T23:59:59.000Z

    . I. o? Service whose personnel located potenI! al study sitcrs atrd us I sted with p! ant identif'ication and profile c!escripi iona. Mt. Dnn pard. 'I e- ton and Mr. Gordon McKee are ac:knowledged for. providing tnchr, rr al guidance. on plant... odlged for hi; ano, 'st. - an!e 'n the ii. "'' 1 p!tave of th'e t'ud, ' Mr. . snd Mxt . Te% Co11irs ere grat 1 ui1y aof! owleoged f!? choir moral aou finar! ial support nf this proj cr t! wi thout which th& o study could not have been completed...

  20. HIGH ABUNDANCES OF PRESOLAR SILICATES IN ANTARCTIC MICROMETEORITES; IMPLICATIONS FOR THEIR COMETARY ORIGINS. T. Yada1,2,3

    E-Print Network [OSTI]

    Syowa Station, in East Antarctica [8, 9]. Seven fine-grained AMMs, T98NF2, -H3, -H5, -G6, -G8, TT54B397 silicates in T98H5 (grains 1P1, 4P1 and 15P1), three Group 4 presolar silicates in T98G8 (grains 4P1, 8P1 been revised to be 50 ppm. For three presolar-silicate-bearing AMMs, TT54B397, T98H5, and G8

  1. A preliminary study of the distribution of some copepods in upper Laguna Madre

    E-Print Network [OSTI]

    Henderson, John C

    1958-01-01T23:59:59.000Z

    LIBRARY a a at cniiEr~ nF TExas A PRELIMINARY STUDY OF THE DISTRIBUTION OF SOME COPEPODS IN UPPER LAGUNA MADRE John C. Henderson A Thesis Submitted to the Graduate School of the Agricultural and Mechanical College of Texas in partial... fulfillment of the requirements for the degree of MASTER OF SCIENCE Mlny, io(R Ma/or Sub)ect: Oceanography A PRELIMINARY STUDY OF THE DISTRIBUTION OF SOME COPEPODS IN UPPER LAGUNA MADRE A Thesis John C. Henderson Approved as to style and content by: C...

  2. First results from $2+1$ dynamical quark flavors on an anisotropic lattice: light-hadron spectroscopy and setting the strange-quark mass

    SciTech Connect (OSTI)

    Lin, Huey-Wen; Cohen, Saul; Dudek, Jozef; Edwards, Robert; Joo, Balint; Richards, David; Bulava, John; Foley, Justin; Morningstar, Colin; Engelson, Eric; Wallace, Stephen; Juge, Jimmy; Mathur, Nilmani; Peardon, Michael; Ryan, Sinead

    2009-02-01T23:59:59.000Z

    We present the first light-hadron spectroscopy on a set of $N_f=2+1$ dynamical, anisotropic lattices. A convenient set of coordinates that parameterize the two-dimensional plane of light and strange-quark masses is introduced. These coordinates are used to extrapolate data obtained at the simulated values of the quark masses to the physical light and strange-quark point. A measurement of the Sommer scale on these ensembles is made and the performance of the hybrid Monte Carlo algorithm used for generating the ensembles is estimated.

  3. Life history and status of the olivaceous cormorant

    E-Print Network [OSTI]

    Morrison, Michael Louis

    1977-01-01T23:59:59.000Z

    LIFE HISTORY AND STATUS OF THE OLIVACFOUS CORMORANT A Thesis by MICHAEL LOUIS MORRISON Submi t ted to the Graduate Col '1 ege nf Texas A~M University in par. jal ful f i 1 jm. . nt of the r equi r, ment fc the d gree of MASTER OF SCIENCE... Oecember 1 977 I, ( jo" Sul-, jey, Nj jdj if ayd y js ' yves 5 jane os LIFE HISTORY AND STATUS OF THE OLIVACEOUS CORMORANT A Thesis by MICHAEL LOUIS MORRISON Approved as to style and content by: Chairma of Committee Member ea of Department Me ber...

  4. Approaching Conformality

    E-Print Network [OSTI]

    Maria Paola Lombardo; Kohtaroh Miura; Tiago Nunes da Silva; Elisabetta Pallante

    2014-11-06T23:59:59.000Z

    We investigate the preconformal region of the phase diagram of SU(3) theories with fundamental flavors. We have simulated SU(3) theories with six and eight fundamental flavors at volumes 32^3 x 64. We use the generated configurations to measure the string tension \\sigma and the w0 scale setting quantity extracted from the gradient flow. We show preliminary results on the ratios Tc/\\sqrt{\\sigma} and Tc w0. We compare them to the behavior obtained at smaller Nf and discuss the implications of our results.

  5. Kalman prediction with Pitch Compensating Quantization for ADPCM voice coding

    E-Print Network [OSTI]

    Berglund, Victor Paul

    1978-01-01T23:59:59.000Z

    18 '. 0. 4 532. 12 3802. 6 873, . 6213. SENTENCE N 2 "ADD THE SUN TO THE PRODUCT OF THESE THREE" &FEMALE) z. FILTER 8 ANDN IDTH: 200. HZ SANPLE NIUNGER 0 h I I?Q FREQUENCY 5ii iQ :"'I I QQ :f rr~rrir'~Nf%"~h. "r:". 'Slf..., I 0 4l"'II. . I j! 4. il II I 5l IIJIJ 55QQ C=. QI! 0 . -";5!! I I I IQQ ? g r ;h?4? ; . :;r?v) IP . . . WX4eWC vA0!0)kb?kkh4?X4?L: x:. ". . . . . -. ". ". -. ". -. ". -. ". ::::: x: x x xi::: i...

  6. Anger camera image generation with microcomputers

    E-Print Network [OSTI]

    Williams, Karl Morgan

    1988-01-01T23:59:59.000Z

    ANGER CAMERA IMAGE GENERATJON WITH iMICR0C0MPJJTERS A Thesis R, 'RI. MGVGAN NIJ. J. IAPLS SubiniL', ti tc: tbe ' taNua - CnlILEr nf Hnivetui ty ;-'r' Lal . '. . '. 6i', i. -ent oL th: ie9uite. . ;List. )i Lhe 4 El'. : ol MASTER 09 SC ENCE May... that with the u e of an innovat ive 'nterfsce system and appropriate software, a microcomputer was a). le tc acquire, display, and analyze Anger came;a images. The interface board designed for this projec. includes A ? D convert. era, a wo page memory system...

  7. The matching of polymer solution fast filament stretching, relaxation and break up experimental results with 1D and 2D numerical viscoelastic simulation

    E-Print Network [OSTI]

    Vadillo, D.C.; Tembely, M.; Morrison, N.F.; Harlen, O.G.; Mackley, M.R.; Soucemarianadin, A.

    D. C. Vadillo*1, M. Tembely**2, N.F. Morrison3, O. G. Harlen3, M. R. Mackley1 10 and A. Soucemarianadin***2 11 12 1Department of Chemical Engineering and Biotechnology, University of Cambridge, 13 CB2 3RA, UK 14 2Laboratory for Geophysical... . Rasmussen, P-Y. Longin, and O. Hassager, Growth of non-635 axisymmetric disturbances of the free surface in the filament stretching rheometer: 636 Experiments and simulation, J. Non-Newtonian Fluid Mech. 180, 163186 (2002). 637 Bazilevsky A. V., V. M...

  8. Fission induced by nucleons at intermediate energies

    E-Print Network [OSTI]

    Sergio Lo Meo; Davide Mancusi; Cristian Massimi; Gianni Vannini; Alberto Ventura

    2014-09-17T23:59:59.000Z

    Monte Carlo calculations of fission of actinides and pre-actinides induced by protons and neutrons in the energy range from 100 MeV to 1 GeV are carried out by means of a recent version of the Li\\`ege Intranuclear Cascade Model, INCL++, coupled with two different evaporation-fission codes, GEMINI++ and ABLA07. In order to reproduce experimental fission cross sections, model parameters are usually adjusted on available (p,f) cross sections and used to predict (n,f) cross sections for the same isotopes.

  9. Election in complete asynchronous networks with intermittent link failures

    E-Print Network [OSTI]

    Lokre, Jahnavi V.

    1991-01-01T23:59:59.000Z

    . Initially, no node knows the identifier of any other node. Each node u knows the number of nodes n in the distributed system, the maximum number f of faulty links incident on u, and that the network is complete. A distributed algorithm on a network is a.... The algorithm uses O(n + nf ) messages. We assume that there are at most f faulty links incident on each node, where f & [ "s J. Let I be a link between nodes u and v. By the definition of link failure, link I is considered faulty if I loses at least one...

  10. A study of the cost of producing peanuts in the western cross timbers farming area of Texas

    E-Print Network [OSTI]

    McArthur, Wilmoth Charles

    1947-01-01T23:59:59.000Z

    e XX Labor anl Poem ~nIrcnonta for Produahg Peannbs . o -t . ~ IXXe C0858 nf Ploclnatston eioooeoee'eeeoeooeeoeee?aeoo ~ eteeto ~ ~ eae 32 IVe ~ oat ~ tet ~ ~ oeetettttt ~ tettttte?teeet ~ etteo ~ t ~ ~ ~ ~ ~ ~ oto 1 kverage knnusl Rainfall... ~ sotto ~ oooo ~ et t ~ o ~ o ~ ~ ~ ~ oe 12 Labor and Poma RezZuired Per k. ore To Perfoxzx the Usual Cperatkons ill Prod1zxdng PC4uts With Traoter and Heres Poser sooooesoaseeo ~ 14 6. Labor and Power Required To Prepsxe the Seel Bed + Sifferent...

  11. A comparative study of the mineral content of vitamin containing foods

    E-Print Network [OSTI]

    Houston, James Maurice

    1936-01-01T23:59:59.000Z

    substituted for the spcaified ssmgie, 4, ". 'etsrniantlon nf ?hosphorue by:. epnrntion nn 'ngnesiukc . ', mmniun . hosphste nn4 . sti ?stion of the . hosphnte ns & hosphoi olybdnte Lhls mthod differs fro. " tkint sudkkcnted by yoe in tso ruspucts, 5 i... out, using 85 oc of - in place of ths sui~ostcd ner. i le for the aothnd outline4 hy toe. 11 10 ~ "otrruin~tion of '* nncsium ny . spar. tian es a;"cesium . ~as?, iete and sti! stion of thn, haspi ate as . hosphomrlybdars xcopt far usi, r ' co...

  12. Scion dominance and related factors influencing compatability in citrus graft unions

    E-Print Network [OSTI]

    Cintron, Rafael H.

    1946-01-01T23:59:59.000Z

    SCION DOMINANCE AND RELATED FACTORS INFLUENCING COMPATIBILITY IN CITRUS GRAFT UNIOHS A The?ia SC H? Clntr&i August 194C Approval as to atyl* and content recommended* m t m m m m m m belated factors nfHOTQiua compatibilitt in cithus m m r... Major Subjectt Horticulture By Rafael H* Clntr&n August 1946 SCI01T DOHIH/ffiCB AND RELATED FACTORS INFLUENCING COMPATIBILITY ITT CITRUS GR APT UNIONS 14-f 706 'The writer1 wishes to express M s sincere approeiation to Dr* Guy W? Adrianee# Head...

  13. Swine as an alternative enterprise: Mixed Soils Area of West Texas

    E-Print Network [OSTI]

    Masch, William Ray

    1970-01-01T23:59:59.000Z

    Directed by: Dr. D. F. . Farris Operators of irrigated farms in the Hixed Soils Area of tJest Texas face a very serious decline of Che underground water supply. As this decline conCinues alt. amative crop and/or livestock enter- prises tu s... important change in these cost and/or prier-s would affect swine's contribution to net famn income. ACKNOWLEDGEHENTS I am espccia1. ly indebted to Dr. D, E, Farris, chairman nf my advisory co smittee, for bis guidencr and assistance in the develop- ment...

  14. Determination of the properties of nuclear energy levels using resonance-scattered neutron capture gamma rays

    E-Print Network [OSTI]

    McCormick, Russell A

    1968-01-01T23:59:59.000Z

    GETElc&ii!'iV! cbN O. ' '! IE FROPENT1ES OF NUCI. EAIl El(E"". GY I. EYEc S USING GESOlIANCE-SCATTEIIEG NE'll'HGN . APTUPZ GAIIMA RAYS A !iles ls IU!Sb "ebb A. Mc. COFci!1CK SubmitteU to the Graclua!. e Colleoo or" Texas Afoul&! Uniser ity... RUSSELL A. PicCOPMTCK Approved "s to stylo and content I?: (CiInirman oE Coaaaittoe V (Member plead nf Dep, . tmont) P! v. oer) (Member) Ma&, l968 Detemsination of the properties of Nuclear Bnergy Lese is Osi: 6 Resonance-Scattered deut...

  15. Exposure to As, Cd and Pb-mixture impairs myelin and axon development in rat brain, optic nerve and retina

    SciTech Connect (OSTI)

    Rai, Nagendra Kumar; Ashok, Anushruti [Academy of Scientific and Innovative Research (India); Developmental Toxicology, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR) (India); Rai, Asit; Tripathi, Sachin [Developmental Toxicology, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR) (India); Nagar, Geet Kumar [Endocrinology, CSIR-Central Drug Research Institute (CSIR-CDRI) (India); Mitra, Kalyan [Electron Microscopy Unit, CSIR-CDRI, Lucknow 226001 (India); Bandyopadhyay, Sanghamitra, E-mail: sanghmitra@iitr.res.in [Academy of Scientific and Innovative Research (India); Developmental Toxicology, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR) (India)

    2013-12-01T23:59:59.000Z

    Arsenic (As), lead (Pb) and cadmium (Cd) are the major metal contaminants of ground water in India. We have reported the toxic effect of their mixture (metal mixture, MM), at human relevant doses, on developing rat astrocytes. Astrocyte damage has been shown to be associated with myelin disintegration in CNS. We, therefore, hypothesized that the MM would perturb myelinating white matter in cerebral cortex, optic nerve (O.N.) and retina. We observed modulation in the levels of myelin and axon proteins, such as myelin basic protein (MBP), proteolipid protein, 2?-, 3?-cyclic-nucleotide-3?-phosphodiesterase, myelin-associated glycoprotein and neurofilament (NF) in the brain of developing rats. Dose and time-dependent synergistic toxic effect was noted. The MBP- and NF-immunolabeling, as well as luxol-fast blue (LFB) staining demonstrated a reduction in the area of intact myelin-fiber, and an increase in vacuolated axons, especially in the corpus-callosum. Transmission electron microscopy (TEM) of O.N. revealed a reduction in myelin thickness and axon-density. The immunolabeling with MBP, NF, and LFB staining in O.N. supported the TEM data. The hematoxylin and eosin staining of retina displayed a decrease in the thickness of nerve-fiber, plexiform-layer, and retinal ganglion cell (RGC) count. Investigating the mechanism revealed a loss in glutamine synthetase activity in the cerebral cortex and O.N., and a fall in the brain derived neurotrophic factor in retina. An enhanced apoptosis in MBP, NF and Brn3b-containing cells justified the diminution in myelinating axons in CNS. Our findings for the first time indicate white matter damage by MM, which may have significance in neurodevelopmental-pediatrics, neurotoxicology and retinal-cell biology. - Highlights: As, Cd and Pb-mixture, at human relevant dose, demyelinate developing rat CNS. The attenuation in myelin and axon is synergistic. The optic nerve and brain demonstrate reduced glutamine synthetase. The retina exhibits diminished neurotrophin levels and cellular differentiation. The toxic effect is apoptotic.

  16. Humic acid in drinking well water induces inflammation through reactive oxygen species generation and activation of nuclear factor-?B/activator protein-1 signaling pathways: A possible role in atherosclerosis

    SciTech Connect (OSTI)

    Hseu, You-Cheng [Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan (China); Department of Molecular and Cellular Oncology, University of Texas, MD Anderson Cancer Center, TX 77030 (United States); Senthil Kumar, K.J. [Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan (China); Chen, Chih-Sheng; Cho, Hsin-Ju; Lin, Shu-Wei; Shen, Pei-Chun [Institute of Nutrition, China Medical University, Taichung 40402, Taiwan (China); Lin, Cheng-Wen [Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan (China); Lu, Fung-Jou [Institute of Medicine, Chun Shan Medical University, Taichung 40201, Taiwan (China); Yang, Hsin-Ling, E-mail: hlyang@mail.cmu.edu.tw [Institute of Nutrition, China Medical University, Taichung 40402, Taiwan (China); Department of Molecular and Cellular Oncology, University of Texas, MD Anderson Cancer Center, TX 77030 (United States)

    2014-01-15T23:59:59.000Z

    Humic acid (HA) has been implicated as one of the etiological factors in the peripheral vasculopathy of blackfoot disease (BFD) in Taiwan. However, the underlying pathophysiological mechanisms of BFD are not well defined. In this study, we used an in vitro and in vivo model, in which HA (25200 ?g/mL) activated macrophages to produce pro-inflammatory molecules by activating their transcriptional factors. HA exposure induced NO and PGE{sub 2} production followed by induction of iNOS and COX-2 through NF-?B/AP-1 transactivation in macrophages. In addition, the production of TNF-? and IL-1? was significantly increased by HA. Moreover, HA-induced iNOS and COX-2 expression were down-regulated by the NF-?B and AP-1 inhibitors pyrrolidine dithiocarbamate (PDTC) and Tanshinone, respectively. Furthermore, generations of ROS and nitrotyrosine, as well as activation of the AKT and MAPKs signaling cascades were observed after HA exposure. Specifically, HA-induced NF-?B activation was mediated by ROS and AKT, and that HA-induced AP-1 activation was mediated by JNK and ERK. Notably, HA-mediated AKT, JNK, and ERK activation was ROS-independent. The inflammatory potential of HA was correlated with increased expression of HO-1 and Nrf2. Furthermore, an in vivo study confirms that mice exposed to HA, the serum levels of TNF-? and IL-1? was significantly increased in a dose-dependent manner. This report marks the first confirmation that environmental exposure of HA induces inflammation in macrophages, which may be one of the main causes of early atherogenesis in blackfoot disease. - Highlights: Humic acid (HA) induce pro-inflammatory cytokines and mediators in macrophages. HA-induced inflammation is mediated by ROS and NF-?B/AP-1 signaling pathways. The inflammatory potential of HA correlated with activation of Nrf2/HO-1 genes. HA exposure to mice increased pro-inflammatory cytokines production in vivo. HA may be one of the main causes of early atherogenesis in blackfoot disease.

  17. Oxidation of sewage supernatant liquor by trickling filtration

    E-Print Network [OSTI]

    Styner, Pete

    1956-01-01T23:59:59.000Z

    ', '': . . 195~9 ":, ' '59 9- ", 19. , -: '; '; 15'F9-;: . :: 418 ?: 499'' ', , : P "-. 8'~5, . : ?. ;. 159 9 . -', ::". , $9-. -:. . . : P P$3I8. :;. "-, . -. , :s8138: '439, ": . ::"::14i:, 9', . ::-', , '-- 4N ' -", ';, '. , :" XL' ". . -:, ' 14..., -. ;;. , ';. ;. . . XiqnM, :or es. crest. sn4-;~ss od As jskNsi' ':5xo, nsg4j~s's1'nips ?', :; . , "::, ' ', : digssegos emik mme-, el@, -er&kling-;Blear srs -eaiu nnies=smpg+sk'in ehs bra semsne. ok' ehs 'soles~, shd erssemiame nf ehs 1@n$4, 'rsspsnei'vole A eriohlkng...

  18. A hypothesis concerning the distribution of salt and salt structures in the Gulf of Mexico

    E-Print Network [OSTI]

    Antoine, John Woodworth

    1970-01-01T23:59:59.000Z

    . oils an. l domes on abyssal plain anil in Bay ot' Cainp che as surveyei. '. by iA'orzel et al. (1968). Inse t is cioss-section A-A' bas d cn. seismic refracton me" sure- ments (Eiving et al. , 1960; Antoine and Ev:ing, 1963) 26 Known and inferred...) shows a re tricted oattern in the central and south!vest. rn parts of the G!!1! of !nf xico. Th= v'ell-defined caste" n and s out( em boundari. s o. th-. kr!oil-and-dome province apparently are associated, w"th th cutline of the Can peche Bank. Th...

  19. Germination of yaupon (Ilex vomitoria Ait.) seed

    E-Print Network [OSTI]

    Fleming, Carl Michael

    1970-01-01T23:59:59.000Z

    !naast For. Er. p. Sta. , Asheville, N. C. 3 pp. Hur. . e, H. H. 1953. Ho! lies. The Hacr&illan Co. tier; York, N. Y. 242 pp. Ives, S. A. 1923. ?Iaturat. ion and germinacion of seed= of Iie', ; op&zom. Bot. Gaz. 76:60-77. Jensen, E. R. 1955. A study... of days required for various level nf germination of excised yaupon seed in response to gibberel]ic acid. 37 xiii LISl' OF FIGURES Figure 1. Longitudinal cross-sections ot yaupon seed -embryos harvested 9-15-66 (upper), and 3-15-67 (lower). 450X 2...

  20. Lipid Metabolism, Gene Expression, Substrate Oxidation, and Meat Quality of Growing-finishing Pigs Supplemented with Conjugated Linoleic Acid and Arginine

    E-Print Network [OSTI]

    Go, Gwang-Woong, 1979-

    2012-02-14T23:59:59.000Z

    NRF-1 Nuclear respiratory factor 1 NF?B Nuclear factor-?B NO Nitric oxide NOS Nitric oxide synthase PGC-1 Peroxisome proliferator-activated receptor gamma coactivator 1 PPAR Peroxisome proliferator-activated receptor PSE Pale, soft... is necessary for mitochondrial biogenesis in response to oxidation of substrate. Hela cells, expressing eNOS, displayed increases in mitochondrial DNA content, cytochrome c oxidase, and the mRNA of PGC-1? and NRF-1 (32). Arginine increased CPT-1A and PGC-1...

  1. A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae.

    E-Print Network [OSTI]

    Baker, Dean A; Nolan, Tony; Fischer, Bettina; Pinder, Alex; Crisanti, Andrea; Russell, Steven R

    2011-06-07T23:59:59.000Z

    -wide analysis of gene expression in adult Anopheles gambiae. Insect Mol Biol 2006, 15:1-12. 3. Dana AN, Hong YS, Kern MK, Hillenmeyer ME, Harker BW, Lobo NF, Hogan JR, Romans P, Collins FH: Gene expression patterns associated with blood-feeding in the malaria... 2005, 208:3971-86. 5. Calvo E, Pham VM, Lombardo F, Arc B, Ribeiro JM: The sialotranscriptome of adult male Anopheles gambiae mosquitoes. Insect Biochem Mol Biol 2006, 36:570-5. 6. Koutsos AC, Blass C, Meister S, Schmidt S, MacCallum RM, Soares MB...

  2. Steer Feeding

    E-Print Network [OSTI]

    Burns, John C.

    1915-01-01T23:59:59.000Z

    COMMITTEE L. J. HART. Chairman J. S. WILLIAMS W. A. MILLER, JR. GOVERNING BOARD, STATE SUBSTATIONS ............................................................................ p L. DOWNS, President Temple.. Term expires 191%) C~ARLES ROGAN, Vice... ~fite ~heAist R. H. RIDGELL, S. As~i~f?nf Chemist SUBSTATION NO. 1: Beeville, Bee County FRANK HODGES. B. 3.. As.slstant Chemist E. E. BINFORD, B. S.. Superintendent W. T. P. SPROTT, B. S.. Ass~stant Chemist SUBSTATION NO. 2: T~~~~, smith county...

  3. The chiral transition in two-flavor QCD

    E-Print Network [OSTI]

    M. D'Elia; A. Di Giacomo; C. Pica

    2005-10-03T23:59:59.000Z

    QCD with N_f=2 is a specially interesting system to investigate the chiral transition. The order of the transition has still not been established. We report the results of an in-depth numerical investigation performed with staggered fermions on lattices with L_t=4 and L_s=12,16,20,24,32 and quark masses am_q ranging from 0.01335 to 0.307036. Using finite-size techniques we compare the scaling behavior of a number of thermodynamical susceptibilities with the expectations of O(4) and O(2) universality classes. Clear disagreement is observed. Indications of a first order transition are found.

  4. An analysis of tritium and fissile fuel exchange in fusion-fission systems

    E-Print Network [OSTI]

    Rice, Brent Lee

    1987-01-01T23:59:59.000Z

    power increases with time. The subscript 1 and 2 utilized in Fig. 1 refer to the fusile and fissile fuels, respectively. The subscripts H, F, and T refer to the fusion reactor, the fission power Fissile Trrtium (1-a) (CH CR1) RH Frssion Power... and fission power reactors, respectively, are: 13 d NT2 (C 1 ) R + a (C C 1 ) R dt (7) d NF2 (C -1) R + (1-a) (C -C ) R dt where N denotes the fuel inventory of a particular system component. C&1 and CT1 denote the number of tritium atoms produced...

  5. Thermodynamics using p4-improved staggered fermion action on QCDOC

    E-Print Network [OSTI]

    Chulwoo Jung

    2005-10-05T23:59:59.000Z

    We present an exploratory study of the thermodynamics of $N_f=3$ QCD with an improved staggered fermions using the QCDOC supercomputer. We use a p4 action with MILC-style smeared links (Fat 7). Some details of the implementation of the p4 action on QCDOC are discussed and performance benchmarks are given. We show preliminary results for the quark mass dependence of the pseudo-critical temperature $T_c$ from several lattice volumes . We also make a comparison between p4fat7 and the old p4 action.

  6. Storage effects on desorption efficiencies of methyl ethyl ketone and styrene collected on activated charcoal

    E-Print Network [OSTI]

    Dommer, Richard Alvin

    1978-01-01T23:59:59.000Z

    in the worker ' s breathir ng zone cont ir uously during his working day. Solid ad- sorbents are used almost exclusively I' or this type of sampling. Among the marr, adsorbents avail able (s ! i ca gel, activated alumina, Tenax, etc. } acti&!ated charcoa&1 I... efficier&cy nf methyl etiiy', Ketone and styrene monomer adsorbed on activated charcoal samples, and stored under isotherm&al condit'ions, were investigated as a function of storage time. The dependence of the storage time effects on the storage temp...

  7. Chiral transition in a strong magnetic background

    E-Print Network [OSTI]

    Eduardo S. Fraga; Ana Jlia Mizher

    2008-04-09T23:59:59.000Z

    The presence of a strong magnetic background can modify the nature and the dynamics of the chiral phase transition at finite temperature. We compute the modified effective potential in the linear sigma model with quarks to one loop in the $\\bar{MS}$ scheme for $N_{f}=2$. For fields $eB\\sim 5 m_{\\pi}^{2}$ and larger a crossover is turned into a weak first-order transition. We discuss possible implications for non-central heavy ion collisions at RHIC and LHC, and for the primordial QCD transition.

  8. Nepali Aawaz Volume 1, Issue 19, 7 December 2006

    E-Print Network [OSTI]

    Shrestha, Kashish Das

    O{lnDa"xnfO{ ;fy lng' k/]sf] lyof] . hftLo :jfoQtf / cfTdlg0f{osf] clwsf/ cyf{t\\ 5'} ls/fFt /fHosf] ;kgf b]vfP/ dfcf]jfbLn] vDa"jfg /fli6o df]rf{ / lnDa"jfg /fli6o d'lQm df]rf{ xft lng ;kmn eP . t/ clxn] oxL If]qdf /fO{ / lnDa' :jfoQtfsf nflu lsrnf] v8f e...

  9. Non-perturbative Test of the Witten-Veneziano Formula from Lattice QCD

    E-Print Network [OSTI]

    Cichy, Krzysztof; Jansen, Karl; Ottnad, Konstantin; Urbach, Carsten

    2015-01-01T23:59:59.000Z

    We compute both sides of the Witten-Veneziano formula using lattice techniques. For the one side we perform dedicated quenched simulations and use the spectral projector method to determine the topological susceptibility in the pure Yang-Mills theory. The other side we determine in lattice QCD with $N_f=2+1+1$ dynamical Wilson twisted mass fermions including for the first time also the flavour singlet decay constant. After taking the continuum and the SU$(2)$ chiral limits we compare both sides and find good agreement within uncertainties.

  10. Resistivity of low percentage perylene to iodine complexes and their excess noise spectra

    E-Print Network [OSTI]

    Caruthers, Jerald Wayne

    1966-01-01T23:59:59.000Z

    that pressed prlycrystalline samples and single crystals should yield the same activation energy. & However others have ~btained activation energies nf 1 to 1 . 5 ev i'nr single crystal s. 9 & 3 L511er reports a rapid droop in activation energy from 0. 7... 0. 60 0. 34 0. 13 0. 35 0. 24 Extrinsic 0, 034 0. 034 0. 056 0, 059 (oZ) 240 250 288 Int. 10 3 10 4 0. 2 50 0. 2 106 10 104 10 Table 1. Information Cbtained from Figures e through P, 16 decreasing the gap between them...

  11. Neutron fluence effects on SC coils and comments

    E-Print Network [OSTI]

    McDonald, Kirk

    SH#3 SH#4 SC#1-5 SC#6-10 SC#11-15 SC#1 7.9W 2.0W 1.0W 0.7W 0.9W 1.4W COMET NF/MC Same size SC wires to increase the SC wire temperature (may be up to the room temperature) to recover their property by anneal on SC#6-10 : 3.5 days HTS instead of resistive magnets looks no hope. #12;Anneal Effect: SC -Tc

  12. Vaccinia Virus Protein A49 is an Unexpected Member of the B-cell Lymphoma (Bcl)-2 Protein Family

    E-Print Network [OSTI]

    Neidel, Sarah; Maluquer de Motes, Carlos; Mansur, Daniel S.; Strnadova, Pavla; Smith, Geoffrey L.; Graham, Stephen C.

    2015-01-20T23:59:59.000Z

    regulators of apoptosis (17). While poxvirus Bcl- 2like proteins F1, M11 and N1 all act to inhibit apoptosis, other poxvirus Bcl-2 family proteins have instead evolved to inhibit activation of the transcription factors NF-?B or IRF3 (9,13). Of particular... pipeline. Eight gold sites were identified with occupancy ranging from 0.88 to 0.18. autoSHARP implemented ARP/wARP (28) to build the initial model that was manually improved with COOT (29) and refined using Refmac5 (30). The resultant model was used...

  13. Development and Use of Baseline Monthly Utility Models for Eight Army Installations Around the United States

    E-Print Network [OSTI]

    Reddy, T. A.; Saman, N. F.; Claridge, D. E.; Haberl, J. S.; Turner, W. D.

    1996-01-01T23:59:59.000Z

    3/96-Final Report, p. i ESL-TR-96/03-01 DEVELOPMENT AND USE OF BASELINE MONTHLY UTILITY MODELS FOR EIGHT ARMY INSTALLATIONS AROUND THE UNITED STATES Prepared by: T.A. Reddy, N.F. Saman, D.E. Claridge, J.S. Haberl and W.D. Turner Research Sponsored... and Scope 2 2.1 Objectives 2 2.2 Data provided 3 2.3 Scope of study 7 3.0 Mathematical Basis of Regression Models 8 3.1 Pertinent background 8 3.2 Simple regression models using Emodel 9 3.3 Generation of 95% uncertainty bands for individual months 14 3...

  14. The Oldham Notebooks: an analysis of the development of IVF 1969-1978. IV. Ethical aspects

    E-Print Network [OSTI]

    Johnson, Martin H.; Elder, Kay

    2015-01-01T23:59:59.000Z

    entitled Fertilization of Human Eggs in Vitro: 90 Morals, Ethics and the Law. In this paper, he admits that the MRC had at least a case, 91 saying (p.8): An immediate issue with new clinical methods concerns the ethics of 92 human experimentation... side, and 567 unfortunately the embryo gravitated Into the cornua 568 Whilst understandably in the patients interests, this procedure did make some of 569 the nursing staff uncomfortable (NF, p.30-31). 570 Second, although no paper evidence has...

  15. Delayed neutron yield from fast neutron induced fission of sup 2 sup 3 sup 8 U

    E-Print Network [OSTI]

    Piksaikin, V M; Isaev, S G; Kazakov, L E; Roshchenko, V A; Tertytchnyi, R G

    2001-01-01T23:59:59.000Z

    The measurements of the total delayed neutron yield from fast neutron induced fission of sup 2 sup 3 sup 8 U were made. The experimental method based on the periodic irradiation of the fissionable sample by neutrons from a suitable nuclear reaction had been employed. The preliminary results on the energy dependence of the total delayed neutron yield from fission of sup 2 sup 3 sup 8 U are obtained. According to the comparison of experimental data with our prediction based on correlation properties of delayed neutron characteristics, it is concluded that the value of the total delayed neutron yield near the threshold of (n,f) reaction is not a constant.

  16. Overcoming cancer therapy resistance by targeting inhibitors of apoptosis proteins and nuclear factor-kappa B

    E-Print Network [OSTI]

    Dai, Yao; Lawrence, Theodore S.; Xu, Liang

    2008-10-25T23:59:59.000Z

    potently bind to and inhibit caspase-3, -7 and -9 and function as potent apoptosis inhibitors (Figure 1). The second group of BIRPs includes the mammalian proteins Survivin (BIRC5) and Bruce (BIRC6) as well as BIR-containing proteins in yeasts and C.... The inhibitor of apoptosis proteins family Gene Protein BIRC 1 NAIP BIRC 2 cIAP-1 BIRC 3 cIAP-2 BIRC 4 XIAP (ILP1) BIRC 5 Survivin BIRC 6 Bruce (Apollon) BIRC 7 ML-IAP (livin, K-IAP) BIRC 8 ILP2 (TsIAP) Cancer therapy targeting IAP and NF...

  17. The running coupling of 8 flavors and 3 colors

    E-Print Network [OSTI]

    Zoltan Fodor; Kieran Holland; Julius Kuti; Santanu Mondal; Daniel Nogradi; Chik Him Wong

    2015-03-03T23:59:59.000Z

    We compute the renormalized running coupling of SU(3) gauge theory coupled to N_f = 8 flavors of massless fundamental Dirac fermions. The recently proposed finite volume gradient flow scheme is used. The calculations are performed at several lattice spacings allowing for a controlled continuum extrapolation. The results for the discrete beta-function show that it is monotonic without any sign of a fixed point in the range of couplings we cover. As a cross check the continuum results are compared with the well-known perturbative continuum beta-function for small values of the renormalized coupling and perfect agreement is found.

  18. The gradient flow running coupling scheme

    E-Print Network [OSTI]

    Zoltan Fodor; Kieran Holland; Julius Kuti; Daniel Nogradi; Chik Him Wong

    2012-11-14T23:59:59.000Z

    The Yang-Mills gradient flow in finite volume is used to define a running coupling scheme. As our main result the discrete beta-function, or step scaling function, is calculated for scale change s=3/2 at several lattice spacings for SU(3) gauge theory coupled to N_f = 4 fundamental massless fermions. The continuum extrapolation is performed and agreement is found with the continuum perturbative results for small renormalized coupling. The case of SU(2) gauge group is briefly commented on.

  19. Determination of the mutagenic potential of individual and binary mixtures of polycyclic aromatic and nitroaromatic hydrocarbons

    E-Print Network [OSTI]

    Keller, Tracie A

    1987-01-01T23:59:59.000Z

    98. The chemicals tested were: 1. b nzn(a)pyrene (BAP), 2. benzo(e)pyrene (BEP), 3. 1-nitropyrene (INP), 4. 2-nitrofluorene (2NF), 5. 7, 12-dimethylbenzanthracene (D11BA), 6. 1, 2-henzanthracene (BA), 7. fluoranthene (FA), and 8. Chrysene (CH... the toxicity of the individual and binary mixtures of chemicals is the Ames Reverse Muta- tion Assay. In this assay, bacteria are mixed with the chemical and tested both with and without the presence of metabolic activation (S9 mix). This mixture is plated...

  20. Non-Abelian Webs of Walls

    E-Print Network [OSTI]

    Minoru Eto; Youichi Isozumi; Muneto Nitta; Keisuke Ohashi; Norisuke Sakai

    2005-09-15T23:59:59.000Z

    Domain wall junctions are studied in N=2 supersymmetric U(Nc) gauge theory with Nf(>Nc) flavors. We find that all three possibilities are realized for positive, negative and zero junction charges. The positive junction charge is found to be carried by a topological charge in the Hitchin system of an SU(2) gauge subgroup. We establish rules of the construction of the webs of walls. Webs can be understood qualitatively by grid diagram and quantitatively by associating moduli parameters to web configurations.

  1. Two-dimensional, subsonic, flowfield analysis for arbitrary boundaries with lifting bodies

    E-Print Network [OSTI]

    Noack, Ralph Wayne

    1981-01-01T23:59:59.000Z

    and negative circula- tion strengths, respect1vely INTRODUCTION The use of a wind tunnel to measure the aerodynam1c properties of a two-d1mens 1onal airfoil yields results which differ from those ob- ta1ned 1n free air. For inviscid flow these d1fferences... in that the freestream or the "1nf1nity" condit1ons must be determ1ned in close proxim1ty to the airfoil (Figure I). Since it 1s desirable to test at Reynolds numbers approximat1ng f11ght conditions, the trend has been to increase the model size. For a f1xed wind...

  2. Infrared spectra of hydrogen-bonded systems using a computer controlled color center laser

    E-Print Network [OSTI]

    Eue, William Charles

    1981-01-01T23:59:59.000Z

    . A Micromation 2-64 minicomputer was chosen to provide interactive control of the laser system. An interface board was designed using 4N33 optical isolaters to prevent voltage spikes from entering the computer, and an SAA-1 027 driver for bi... RB +12 volts IC2 RS i IC4 STEPPER MOTOR R6 IC3 Figure 6. Stepper Motor Interface. Rl-3: 150 ohms, R4-6: 1000 ohms, R7: 150 ohms, RB: 390 ohms, Cl: . 1 nf, ICI-3: 4N33, IC4: SAA-1027. 13 precision operational amplifiers, Figure 7...

  3. Multiple personality, a case study

    E-Print Network [OSTI]

    Musick, William Daniel Anderson

    1976-01-01T23:59:59.000Z

    . An exhaustive review of the literature revealed n& previous attempts at ut ilizing videotape therapy in the T. ea n&eni of rriultiple personali ty. Hnwever, via?. toping h&, s he, n !ncreosin&3!y llsea with great success in training therapists (Musan... personolity. Ir was also based on the past successes nf audio topes used in conjur!ation w!tn psychoanaiysis and hypnosis in the treat!nant of mal tip lr personalities. Following tt!e agree!nent to instItute videorape therapy inl. ! Ihe treat!ment plan...

  4. Differential of discounted present values of civilian versus military lifetime earnings

    E-Print Network [OSTI]

    Phillips, Christian

    1972-01-01T23:59:59.000Z

    Navy, Marine orps, Air Force, to Vietnam - 0. 1667 0. 0186 0. 0341 ? 0. 477. Assumed the individual is now in Vietnam his chance of getting killed because of actual combat while a member of the Army is ? 0. 0068 Navy ? 0. 0018 Marine Corps- 0... of 1972 ? 53. Probability of being ordered to Vietnar regardless nf branch ? 0. 037&~. Fro', ability of being placed in the Army ? 0. 381$ Navy ? 0. 2II. 03 Plar ine C orps ? 0. 0821 Air ~ orcc - 0. 2050. Once in the Army, probability of' going...

  5. Chow Wagons

    E-Print Network [OSTI]

    Unknown

    2011-09-05T23:59:59.000Z

    . Horlacher, Ph. D., Genetics D. Scoates, A. E.. Agricultural Engineering J. H. Knox, M. S., Animal Husbandry A. K. Mackey. M. S., Animal Husbandry A. L. Darnell, M. A., Dairy Husbandry *Dean School of Veterinary Medicine. ?As of March 1. 1932. **In coo... difference of $12.17 in favor machine harvesting. !on- ton. ent :ive the 'er - ,C V1 ant a re- . . ars 'ull In nd - YZU In age tad. de. ale ith nf 11Vb ste- nic- BULLETIN NO. 452 AUGUST, 1932 THE MECHANICAL HARVESTING OF COTTON H...

  6. The leading disconnected contribution to the anomalous magnetic moment of the muon

    E-Print Network [OSTI]

    Anthony Francis; Vera Glpers; Benjamin Jger; Harvey Meyer; Georg von Hippel; Hartmut Wittig

    2014-11-27T23:59:59.000Z

    The hadronic vacuum polarization can be determined from the vector correlator in a mixed time-momentum representation. We explicitly calculate the disconnected contribution to the vector correlator, both in the $N_f = 2$ theory and with an additional quenched strange quark, using non-perturbatively $O(a)$-improved Wilson fermions. All-to-all propagators are computed using stochastic sources and a generalized hopping parameter expansion. Combining the result with the dominant connected contribution, we are able to estimate an upper bound for the systematic error that arises from neglecting the disconnected contribution in the determination of $(g-2)_\\mu$.

  7. Microsoft Word - Comments received at White Rock Scoping Mtg.doc

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis and FeedbackProgrammatic5154:9:CMRR-NF

  8. Microsoft Word - Contact List Template 11-5-14- ali notes.doc

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis and FeedbackProgrammatic5154:9:CMRR-NF IAEA

  9. Microsoft Word - Cover Sheet - 020711

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis and FeedbackProgrammatic5154:9:CMRR-NF

  10. Microsoft Word - Cover Sheet.doc

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis and FeedbackProgrammatic5154:9:CMRR-NF1, Book

  11. Microsoft Word - DE-NR0000031-001.rtf

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis and FeedbackProgrammatic5154:9:CMRR-NF1,

  12. Microsoft Word - DE-NR0000031-075.doc

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOn AprilA Approved:AdministrationAnalysis and FeedbackProgrammatic5154:9:CMRR-NF1,ITEM

  13. IEA Wind Task 26: Wind Technology, Cost, and Performance Trends in Denmark, Germany, Ireland, Norway, the European Union, and the United States: 2007…2012

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh School footballHydrogen andHypernuclei in Hall CInbydoeCELLIDRMSIDS-NF

  14. NGP Energy Technology Partners | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF Energy Saving

  15. NIACC John Pappajohn Entrepreneurial Center JPEC | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF Energy SavingNIACC

  16. NIEX Incubator | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF Energy

  17. NM Installation Requirements for UST Systems | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF EnergyInstallation

  18. NM Stat. 62-10 - Hearings Before the Commission | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF

  19. NM Stat. 62-11 - Review of Commission Orders | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF- Review of

  20. NM Stat. 62-9 - The Utility Franchise | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF- Review ofNM Stat.

  1. NM Underground Storage Tank Registration | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF- Review ofNM

  2. NMAC 1.2.2 Public Regulations Commission Rules of Procedure | Open Energy

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF- Review

  3. NMAC 17.1.2 Utility Applications | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF- ReviewApplications

  4. NMAC 17.5.440 Extensions, Improvements, Additions, and Cooperative

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-

  5. NMAC 17.9.568 Interconnection of Generating Facilities with a Rated

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up to and

  6. NMAC 17.9.569 Interconnection of Generating Facilities with a Rated

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up to

  7. NMAC 19.14 Geothermal Power | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up to9.14

  8. NMAC 19.14.1 Geothermal Power General Provisions | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up to9.141

  9. NMAC 19.14.21 Geothermal Power Drilling Permit | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up

  10. NMAC 19.14.23 Geothermal Power Well Spacing | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up23

  11. NMAC 19.14.54 Geothermal Power Sundry Notices and Reports on Geothermal

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity up23Well

  12. NMAC 19.14.55 Certificate of Compliance and Authorization to Produce

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer PlantMunhall, Pennsylvania: Energy Resources JumpNEF Advisors LLC JumpNF-Capacity

  13. Section 27

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF o N e

  14. Section 27

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF o N

  15. Section 27: Peer Review

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF o

  16. Section 28

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF

  17. Section 29

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF5

  18. Section 29

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF57

  19. Section 3

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF578 '

  20. Section 3

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF578 '

  1. Section 30

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over Our Instagram Secretary Moniz is Taking Over OurSecretaryFigureAtmosphericOnNF578

  2. Orientifold Planar Equivalence: The Chiral Condensate

    E-Print Network [OSTI]

    Adi Armoni; Biagio Lucini; Agostino Patella; Claudio Pica

    2008-09-29T23:59:59.000Z

    The recently introduced orientifold planar equivalence is a promising tool for solving non-perturbative problems in QCD. One of the predictions of orientifold planar equivalence is that the chiral condensates of a theory with $N_f$ flavours of Dirac fermions in the symmetric (or antisymmetric) representation and $N_f$ flavours of Majorana fermions in the adjoint representation have the same large $N$ value for any value of the mass of the (degenerate) fermions. Assuming the invariance of the theory under charge conjugation, we prove this statement on the lattice for staggered quenched condensates in SU($N$) Yang-Mills in the large $N$ limit. Then, we compute numerically those quenched condensates for $N$ up to 8. After separating the even from the odd corrections in $1/N$, we are able to show that our data support the equivalence; however, unlike other quenched observables, subleading terms in $1/N$ are needed for describing the data for the symmetric and antisymmetric representation at $N$=3. Possible lessons for the unquenched case are discussed.

  3. Etching process of silicon dioxide with nonequilibrium atmospheric pressure plasma

    SciTech Connect (OSTI)

    Yamakawa, Koji; Hori, Masaru; Goto, Toshio; Den, Shoji; Katagiri, Toshirou; Kano, Hiroyuki [Department of Quantum Engineering, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603 (Japan); Department of Electrical Engineering and Computer Science, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603 (Japan); Department of Quantum Engineering, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603 (Japan); Department of Engineering, Katagiri Engineering Co., Ltd., Minamikase Saiwai-ku, Kawasaki 661-8661 (Japan); NU-EcoEngineering Co., Ltd., Ooaza, Kurozasa Aza, Umazutsumi, Miyoshi-cho, Nishikamo-gun, Aichi 470-0201 (Japan)

    2005-07-01T23:59:59.000Z

    An ultrahigh etch rate (14 {mu}m/min) of SiO{sub 2} and a high selectivity of SiO{sub 2}/Si over 200 were achieved using a microwave-excited nonequilibrium atmospheric pressure plasma source employing He, NF{sub 3}, and H{sub 2}O gases, which have been developed for application to microelectromechanical systems and other bionanotechnology fields. In order to clarify the etching mechanism, two diagnostic methods have been performed: (1) imaging of plasma emission with an intensified charge-coupled device camera, and (2) absorption measurements using Fourier transform infrared spectroscopy. The etching characteristics are discussed in relation to the spatial distributions of the species involved. The etch rate depended considerably on the distance between the plasma and the substrate. Some radicals generated from the feed gases reached the substrate directly, while other radicals recombined into different species, which reached the substrate. An abundance of HF molecules were produced through a reaction between radicals generated by the atmospheric pressure discharge of NF{sub 3} and H{sub 2}O. From these measurements, it has been found that the HF molecules generated played a role in producing the high etch rate of SiO{sub 2} and high etch selectivity of SiO{sub 2}/Si.

  4. A multichannel magnetic probe system for analysing magnetic fluctuations in helical axis plasmas

    SciTech Connect (OSTI)

    Haskey, S. R.; Blackwell, B. D.; Seiwald, B.; Hole, M. J.; Pretty, D. G.; Howard, J.; Wach, J. [Plasma Research Laboratory, Research School of Physical Sciences and Engineering, The Australian National University, Canberra, ACT 0200 (Australia)] [Plasma Research Laboratory, Research School of Physical Sciences and Engineering, The Australian National University, Canberra, ACT 0200 (Australia)

    2013-09-15T23:59:59.000Z

    The need to understand the structure of magnetic fluctuations in H-1NF heliac [S. Hamberger et al., Fusion Technol. 17, 123 (1990)] plasmas has motivated the installation of a sixteen former, tri-axis helical magnetic probe Mirnov array (HMA). The new array complements two existing poloidal Mirnov arrays by providing polarisation information, higher frequency response, and improved toroidal resolution. The helical placement is ideal for helical axis plasmas because it positions the array as close as possible to the plasma in regions of varying degrees of favourable curvature in the magnetohydrodynamic sense, but almost constant magnetic angle. This makes phase variation with probe position near linear, greatly simplifying the analysis of the data. Several of the issues involved in the design, installation, data analysis, and calibration of this unique array are presented including probe coil design, frequency response measurements, mode number identification, orientation calculations, and mapping probe coil positions to magnetic coordinates. Details of specially designed digitally programmable pre-amplifiers, which allow gains and filters to be changed as part of the data acquisition initialisation sequence and stored with the probe signals, are also presented. The low shear heliac geometry [R. Jimnez-Gmez et al., Nucl. Fusion 51, 033001 (2011)], flexibility of the H-1NF heliac, and wealth of information provided by the HMA create a unique opportunity for detailed study of Alfvn eigenmodes, which could be a serious issue for future fusion reactors.

  5. Electron-impact excitation of the Rydberg levels of the oxygen molecule

    SciTech Connect (OSTI)

    Chung, S.; Lin, C.C.; Lee, E.T.

    1990-10-01T23:59:59.000Z

    Electron-impact cross sections for exciting the oxygen molecule from the ground electronic level to the ns sigma sub g, np sigma sub u, nd sigma subg, nf sigma sub u, np pi sub u, nd pi sub g, and nf pi sub u. Rydberg levels associated with the O2+(B2 sigma(-)sub g) and O2(+)(b4 sigma(-) sub g) cores are presented. For each Rydberg series of a given l, m, and O2+ core state, the cross sections exhibit n dependence of the form 1/n to the alpha power with alpha varying from one series to another in the range of 1.47 to 4.60 in contrast to the more universal 1/n-cubed dependence for the hydrogen atom. The potential cusps at the two nuclear sites of the molecule make the Rydberg orbital density near the origin quite different from the hydrogen atom, thereby spoiling the simple 1/n-cubed dependence. (JHD)

  6. A study of the energy evolution of event shape distributions and their means with the DELPHI detector at LEP

    E-Print Network [OSTI]

    The DELPHI Collaboration; J. Abdallah

    2003-07-17T23:59:59.000Z

    Infrared and collinear safe event shape distributions and their mean values are determined in e+e- collisions at centre-of-mass energies between 45 and 202 GeV. A phenomenological analysis based on power correction models including hadron mass effects for both differential distributions and mean values is presented. Using power corrections, alpha_s is extracted from the mean values and shapes. In an alternative approach, renormalisation group invariance (RGI) is used as an explicit constraint, leading to a consistent description of mean values without the need for sizeable power corrections. The QCD beta-function is precisely measured using this approach. From the DELPHI data on Thrust, including data from low energy experiments, one finds beta_0 = 7.86 +/- 0.32 for the one loop coefficient of the beta-function or, assuming QCD, n_f = 4.75 +/- 0.44 for the number of active flavours. These values agree well with the QCD expectation of beta_0=7.67 and n_f=5. A direct measurement of the full logarithmic energy slope excludes light gluinos with a mass below 5 GeV.

  7. Compatibility Assessment of Advanced Stainless Steels in Sodium

    SciTech Connect (OSTI)

    Pawel, Steven J [ORNL

    2012-01-01T23:59:59.000Z

    Type 316L stainless steel capsules containing commercially pure sodium and miniature tensile specimens of HT-UPS (austenitic, 14Cr-16Ni), NF-616 (ferritic/martensitic, 9Cr-2W-0.5Mo), or 316L (austenitic, 17Cr-10Ni-2Mo) stainless steel were exposed at 600 or 700 C for 100 and 400 h as a screening test for compatibility. Using weight change, tensile testing, and metallographic analysis, HT-UPS and 316L were found to be largely immune to changes resulting from sodium exposure, but NF-616 was found susceptible to substantial decarburization at 700 C. Subsequently, two thermal convection loops (TCLs) constructed of 316L and loaded with commercially pure sodium and miniature tensile specimens of HT-UPS and 316L were operated for 2000 h each one between 500 and 650 C, the other between 565 and 725 C at a flow rate of about 1.5 cm/s. Changes in specimen appearance, weight, and tensile properties were observed to be very minor in all cases, and there was no metallographic evidence of microstructure changes, composition gradients, or mass transfer resulting from prolonged exposure in a TCL. Thus, it appears that HT-UPS and 316L stainless steels are similarly compatible with commercially pure sodium under these exposure conditions.

  8. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Cancer Research, Institute for Molecular Biology, College of Life Sciences, Nankai University, Tianjin 300071 (China); Ye, Lihong, E-mail: yelihong@nankai.edu.cn [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China)] [Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071 (China)

    2013-05-03T23:59:59.000Z

    Highlights: HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. HBXIP serves as a coactivator of activating transcription factor Sp1. HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  9. Heavy-quark free energy at finite temperature with 2+1 flavors of improved Wilson quarks in fixed scale approach

    E-Print Network [OSTI]

    Y. Maezawa; S. Aoki; S. Ejiri; T. Hatsuda; K. Kanaya; H. Ohno; T. Umeda

    2009-11-02T23:59:59.000Z

    The free energy between a static quark and an antiquark is studied by using the color-singlet Polyakov-line correlation at finite temperature. We perform simulations on $32^3 \\times 12$, 10, 8, 6, 4 lattices in the high temperature phase with the RG-improved gluon action and 2+1 flavors of the clover-improved Wilson quark action. Since the simulations are based on the fixed scale approach that the temperature can be varied without changing the spatial volume and renormalization factor, it is possible to investigate temperature dependence of the heavy-quark free energy without any adjustment of the overall constant. We find that, the heavy-quark free energies at short distance converge to the heavy-quark potential evaluated from the Wilson-loop operator at zero temperature, in accordance with the expected insensitivity of short distance physics to the temperature. At long distance, the heavy-quark free energies approach to twice the single-quark free energies, implying that the interaction between heavy quarks is screened. The Debye screening mass obtained from the long range behavior of the heavy-quark free energy is compared with results of the thermal perturbation theory and those of $N_f=2$ and $N_f=0$ lattice simulations.

  10. Anti-inflammatory activity of methyl palmitate and ethyl palmitate in different experimental rat models

    SciTech Connect (OSTI)

    Saeed, Noha M. [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Egyptian Russian University, Cairo (Egypt)] [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Egyptian Russian University, Cairo (Egypt); El-Demerdash, Ebtehal [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt)] [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt); Abdel-Rahman, Hanaa M. [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Egyptian Russian University, Cairo (Egypt)] [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Egyptian Russian University, Cairo (Egypt); Algandaby, Mardi M. [Department of Biology (Botany), Faculty of Science, King Abdulaziz University, Jeddah (Saudi Arabia)] [Department of Biology (Botany), Faculty of Science, King Abdulaziz University, Jeddah (Saudi Arabia); Al-Abbasi, Fahad A. [Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah (Saudi Arabia)] [Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah (Saudi Arabia); Abdel-Naim, Ashraf B., E-mail: abnaim@pharma.asu.edu.eg [Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo (Egypt)

    2012-10-01T23:59:59.000Z

    Methyl palmitate (MP) and ethyl palmitate (EP) are naturally occurring fatty acid esters reported as inflammatory cell inhibitors. In the current study, the potential anti-inflammatory activity of MP and EP was evaluated in different experimental rat models. Results showed that MP and EP caused reduction of carrageenan-induced rat paw edema in addition to diminishing prostaglandin E2 (PGE2) level in the inflammatory exudates. In lipopolysaccharide (LPS)-induced endotoxemia in rats, MP and EP reduced plasma levels of tumor necrosis factor-? (TNF-?) and interleukin-6 (IL-6). MP and EP decreased NF-?B expression in liver and lung tissues and ameliorated histopathological changes caused by LPS. Topical application of MP and EP reduced ear edema induced by croton oil in rats. In the same animal model, MP and EP reduced neutrophil infiltration, as indicated by decreased myeloperoxidase (MPO) activity. In conclusion, this study demonstrates the effectiveness of MP and EP in combating inflammation in several experimental models. -- Highlights: ? Efficacy of MP and EP in combating inflammation was displayed in several models. ? MP and EP reduced carrageenan-induced rat paw edema and prostaglandin E2 level. ? MP and EP decreased TNF-? and IL-6 levels in experimental endotoxemia. ? MP and EP reduced NF-?B expression and histological changes in rat liver and lung. ? MP and EP reduced croton oil-induced ear edema and neutrophil infiltration.

  11. Id1 expression promotes peripheral CD4{sup +} T cell proliferation and survival upon TCR activation without co-stimulation

    SciTech Connect (OSTI)

    Liu, Chen; Jin, Rong [Department of Immunology, Peking University Health Science Center, Beijing (China)] [Department of Immunology, Peking University Health Science Center, Beijing (China); Wang, Hong-Cheng [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States)] [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States); Tang, Hui; Liu, Yuan-Feng; Qian, Xiao-Ping; Sun, Xiu-Yuan; Ge, Qing [Department of Immunology, Peking University Health Science Center, Beijing (China)] [Department of Immunology, Peking University Health Science Center, Beijing (China); Sun, Xiao-Hong, E-mail: sunx@omrf.org [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States)] [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States); Zhang, Yu, E-mail: zhangyu007@bjmu.edu.cn [Department of Immunology, Peking University Health Science Center, Beijing (China)] [Department of Immunology, Peking University Health Science Center, Beijing (China)

    2013-06-21T23:59:59.000Z

    Highlights: Id1 expression enables nave T cell proliferation without anti-CD28 co-stimulation. Id1 expression facilitates T cells survival when stimulated with anti-CD3. Elevation of IL-2 production by Id1 contributes increased proliferation and survival. Id1 potentiates NF-?B activation by anti-CD3 stimulation. -- Abstract: Although the role of E proteins in the thymocyte development is well documented, much less is known about their function in peripheral T cells. Here we demonstrated that CD4 promoter-driven transgenic expression of Id1, a naturally occurring dominant-negative inhibitor of E proteins, can substitute for the co-stimulatory signal delivered by CD28 to facilitate the proliferation and survival of nave CD4{sup +} cells upon anti-CD3 stimulation. We next discovered that IL-2 production and NF-?B activity after anti-CD3 stimulation were significantly elevated in Id1-expressing cells, which may be, at least in part, responsible for the augmentation of their proliferation and survival. Taken together, results from this study suggest an important role of E and Id proteins in peripheral T cell activation. The ability of Id proteins to by-pass co-stimulatory signals to enable T cell activation has significant implications in regulating T cell immunity.

  12. Sirtuin inhibition attenuates the production of inflammatory cytokines in lipopolysaccharide-stimulated macrophages

    SciTech Connect (OSTI)

    Fernandes, Claudia A. [Universite catholique de Louvain, Louvain Drug Research Institute (LDRI), Pharmaceutics and Drug Delivery Research Group, Brussels B-1200 (Belgium)] [Universite catholique de Louvain, Louvain Drug Research Institute (LDRI), Pharmaceutics and Drug Delivery Research Group, Brussels B-1200 (Belgium); Fievez, Laurence [University of Liege, GIGA-Research, Laboratory of Cellular and Molecular Immunology, Liege B-4000 (Belgium)] [University of Liege, GIGA-Research, Laboratory of Cellular and Molecular Immunology, Liege B-4000 (Belgium); Neyrinck, Audrey M.; Delzenne, Nathalie M. [Universite catholique de Louvain, LDRI, Metabolism and Nutrition Research Group, Brussels B-1200 (Belgium)] [Universite catholique de Louvain, LDRI, Metabolism and Nutrition Research Group, Brussels B-1200 (Belgium); Bureau, Fabrice [University of Liege, GIGA-Research, Laboratory of Cellular and Molecular Immunology, Liege B-4000 (Belgium)] [University of Liege, GIGA-Research, Laboratory of Cellular and Molecular Immunology, Liege B-4000 (Belgium); Vanbever, Rita, E-mail: rita.vanbever@uclouvain.be [Universite catholique de Louvain, Louvain Drug Research Institute (LDRI), Pharmaceutics and Drug Delivery Research Group, Brussels B-1200 (Belgium)] [Universite catholique de Louvain, Louvain Drug Research Institute (LDRI), Pharmaceutics and Drug Delivery Research Group, Brussels B-1200 (Belgium)

    2012-04-20T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer Lipopolysaccharide-stimulated macrophages were treated with cambinol and sirtinol. Black-Right-Pointing-Pointer Cambinol and sirtinol decreased lipopolysaccharide-induced cytokines. Black-Right-Pointing-Pointer Cambinol decreased NF-{kappa}B activity but had no impact on p38 MAPK activation. Black-Right-Pointing-Pointer Sirtuins are an interesting target for the treatment of inflammatory diseases. -- Abstract: In several inflammatory conditions such as rheumatoid arthritis or sepsis, the regulatory mechanisms of inflammation are inefficient and the excessive inflammatory response leads to damage to the host. Sirtuins are class III histone deacetylases that modulate the activity of several transcription factors that are implicated in immune responses. In this study, we evaluated the impact of sirtuin inhibition on the activation of lipopolysaccharide (LPS)-stimulated J774 macrophages by assessing the production of inflammatory cytokines. The pharmacologic inhibition of sirtuins decreased the production of tumour necrosis factor-alpha (TNF-{alpha}) interleukin 6 (IL-6) and Rantes. The reduction of cytokine production was associated with decreased nuclear factor kappa B (NF-{kappa}B) activity and inhibitor kappa B alpha (I{kappa}B{alpha}) phosphorylation while no impact was observed on the phosphorylation status of p38 mitogen-activated kinase (p38 MAPK). This work shows that sirtuin pharmacologic inhibitors are a promising tool for the treatment of inflammatory conditions.

  13. Structure functions for light nuclei

    SciTech Connect (OSTI)

    S.A. Kulagin, R. Petti

    2010-11-01T23:59:59.000Z

    We discuss the nuclear EMC effect with particular emphasis on recent data for light nuclei including 2H, 3He, 4He, 9Be, 12C and 14N. In order to verify the consistency of available data, we calculate the \\chi^2 deviation between different data sets. We find a good agreement between the results from the NMC, SLAC E139, and HERMES experiments. However, our analysis indicates an overall normalization offset of about 2% in the data from the recent JLab E03-103 experiment with respect to previous data for nuclei heavier than 3He. We also discuss the extraction of the neutron/proton structure function ratio F2n/F2p from the nuclear ratios 3He/2H and 2H/1H. Our analysis shows that the E03-103 data on 3He/2H require a renormalization of about 3% in order to be consistent with the F2n/F2p ratio obtained from the NMC experiment. After such a renormalization, the 3He data from the E03-103 data and HERMES experiments are in a good agreement. Finally, we present a detailed comparison between data and model calculations, which include a description of the nuclear binding, Fermi motion and off-shell corrections to the structure functions of bound proton and neutron, as well as the nuclear pion and shadowing corrections. Overall, a good agreement with the available data for all nuclei is obtained.

  14. Toward a Holographic Model of Superconducting Fermions

    E-Print Network [OSTI]

    Andy O'Bannon

    2008-11-02T23:59:59.000Z

    We use the AdS/CFT correspondence to study N=4 supersymmetric SU(Nc) Yang-Mills theory, in the limits of large Nc and large 't Hooft coupling, coupled to a number Nf of massless hypermultiplet fields in the fundamental representation of the gauge group. We identify a U(1) subgroup of the R-symmetry under which the fermions in the hypermultiplet are charged but the scalars are not. All the hypermultiplet fields are also charged under a U(1) baryon number symmetry. We introduce an external magnetic field for the baryon number U(1), which triggers the spontaneous breaking of the U(1) R-symmetry, and we then introduce a chemical potential for the U(1) R-charge, producing a state with a nonzero density of the U(1) R-charge. The system should then exhibit superconductivity of the U(1) R-charge. The dual supergravity description is a number Nf of D7-branes in AdS5 x S5 with angular momentum on the S5 and a worldvolume magnetic field. We study the zero-temperature thermodynamics of the system, and find that for sufficiently large magnetic field the system prefers to be in the symmetry-broken phase. For smaller magnetic fields we find a discontinuous free energy, indicating that our gravitational setup does not capture all equilibrium states of the field theory.

  15. DRAFT - Design of Radiological Survey and Sampling to Support Title Transfer or Lease of Property on the Department of Energy Oak Ridge Reservation

    SciTech Connect (OSTI)

    Cusick L.T.

    2002-09-25T23:59:59.000Z

    The U.S. Department of Energy (DOE) owns, operates, and manages the buildings and land areas on the Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee. As land and buildings are declared excess or underutilized, it is the intent of DOE to either transfer the title of or lease suitable property to the Community Reuse Organization of East Tennessee (CROET) or other entities for public use. It is DOE's responsibility, in coordination with the U.S. Environmental Protection Agency (EPA), Region 4, and the Tennessee Department of Environment and Conservation (TDEC), to ensure that the land, facilities, and personal property that are to have the title transferred or are to be leased are suitable for public use. Release of personal property must also meet site requirements and be approved by the DOE contractor responsible for site radiological control. The terms title transfer and lease in this document have unique meanings. Title transfer will result in release of ownership without any restriction or further control by DOE. Under lease conditions, the government retains ownership of the property along with the responsibility to oversee property utilization. This includes involvement in the lessee's health, safety, and radiological control plans and conduct of site inspections. It may also entail lease restrictions, such as limiting access to certain areas or prohibiting digging, drilling, or disturbing material under surface coatings. Survey and sampling requirements are generally more rigorous for title transfer than for lease. Because of the accelerated clean up process, there is an increasing emphasis on title transfers of facilities and land. The purpose of this document is to describe the radiological survey and sampling protocols that are being used for assessing the radiological conditions and characteristics of building and land areas on the Oak Ridge Reservation that contain space potentially available for title transfer or lease. After necessary surveys and sampling and laboratory analyses are completed, the data are analyzed and included in an Environmental Baseline Summary (EBS) report for title transfer or in a Baseline Environmental Analysis Report (BEAR) for lease. The data from the BEAR is then used in a Screening-Level Human Health Risk Assessment (SHHRA) or a risk calculation (RC) to assess the potential risks to future owners/occupants. If title is to be transferred, release criteria in the form of specific activity concentrations called Derived Concentration Guideline Levels (DCGLs) will be developed for the each property. The DCGLs are based on the risk model and are used with the data in the EBS to determine, with statistical confidence, that the release criteria for the property have been met. The goal of the survey and sampling efforts is to (1) document the baseline conditions of the property (real or personal) prior to title transfer or lease, (2) obtain enough information that an evaluation of radiological risks can be made, and (3) collect sufftcient data so that areas that contain minimal residual levels of radioactivity can be identified and, following radiological control procedures, be released from radiological control. (It should be noted that release from radiological control does not necessarily mean free release because DOE may maintain institutional control of the site after it is released from radiological control). To meet the goals of this document, a Data Quality Objective (DQO) process will be used to enhance data collection efficiency and assist with decision-making. The steps of the DQO process involve stating the problem, identifying the decision, identifying inputs to the decision, developing study boundaries, developing the decision rule, and optimizing the design. This document describes the DQOs chosen for surveys and sampling efforts performed for the purposes listed above. The previous version to this document focused on the requirements for radiological survey and sampling protocols that are be used for leasing. Because the primary focus at this time is on title transfer, th

  16. Chronic occupational exposure to arsenic induces carcinogenic gene signaling networks and neoplastic transformation in human lung epithelial cells

    SciTech Connect (OSTI)

    Stueckle, Todd A., E-mail: tstueckle@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Lu, Yongju, E-mail: yongju6@hotmail.com [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States); Davis, Mary E., E-mail: mdavis@wvu.edu [Department of Physiology, West Virginia University, Morgantown, WV 26506 (United States); Wang, Liying, E-mail: lmw6@cdc.gov [Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States)] [Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Jiang, Bing-Hua, E-mail: bhjiang@jefferson.edu [Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)] [Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Holaskova, Ida, E-mail: iholaskova@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Schafer, Rosana, E-mail: rschafer@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Barnett, John B., E-mail: jbarnett@hsc.wvu.edu [Department of Microbiology, Immunology and Cell Biology, West Virginia University, Morgantown, WV 26506 (United States); Rojanasakul, Yon, E-mail: yrojan@hsc.wvu.edu [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)] [Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV 26506 (United States)

    2012-06-01T23:59:59.000Z

    Chronic arsenic exposure remains a human health risk; however a clear mode of action to understand gene signaling-driven arsenic carcinogenesis is currently lacking. This study chronically exposed human lung epithelial BEAS-2B cells to low-dose arsenic trioxide to elucidate cancer promoting gene signaling networks associated with arsenic-transformed (B-As) cells. Following a 6 month exposure, exposed cells were assessed for enhanced cell proliferation, colony formation, invasion ability and in vivo tumor formation compared to control cell lines. Collected mRNA was subjected to whole genome expression microarray profiling followed by in silico Ingenuity Pathway Analysis (IPA) to identify lung carcinogenesis modes of action. B-As cells displayed significant increases in proliferation, colony formation and invasion ability compared to BEAS-2B cells. B-As injections into nude mice resulted in development of primary and secondary metastatic tumors. Arsenic exposure resulted in widespread up-regulation of genes associated with mitochondrial metabolism and increased reactive oxygen species protection suggesting mitochondrial dysfunction. Carcinogenic initiation via reactive oxygen species and epigenetic mechanisms was further supported by altered DNA repair, histone, and ROS-sensitive signaling. NF-?B, MAPK and NCOR1 signaling disrupted PPAR?/?-mediated lipid homeostasis. A pro-cancer gene signaling network identified increased survival, proliferation, inflammation, metabolism, anti-apoptosis and mobility signaling. IPA-ranked signaling networks identified altered p21, EF1?, Akt, MAPK, and NF-?B signaling networks promoting genetic disorder, altered cell cycle, cancer and changes in nucleic acid and energy metabolism. In conclusion, transformed B-As cells with their whole genome expression profile provide an in vitro arsenic model for future lung cancer signaling research and data for chronic arsenic exposure risk assessment. Highlights: ? Chronic As{sub 2}O{sub 3} exposure to lung epithelial cells resulted in a cancer-like phenotype. ? Mice injected with arsenic transformed (B-As) cells displayed metastatic tumors. ? Microarray profiling revealed changes in mitochondrial metabolism and ROS response. ? p21, EF1?, Akt, MAPK, PPAR? and NF-?B networks promoted pro-cancer signaling. ? B-As cells represent a lung cancer model to explore As-associated carcinogenesis.

  17. Anti-inflammatory and antifibrotic effects of methyl palmitate

    SciTech Connect (OSTI)

    El-Demerdash, Ebtehal, E-mail: ebtehal_dm@yahoo.com

    2011-08-01T23:59:59.000Z

    Methyl palmitate (MP) has been shown earlier to inhibit Kupffer cells and rat peritoneal macrophages. To evaluate the potential of MP to inhibit the activation of other macrophages, RAW cells (macrophages of alveolar origin) were treated with varying concentrations of MP (0.25, 0.5, 1 mM). Assessment of cytotoxicity using MTT assay revealed that 0.25 and 0.5 mM are not toxic to RAW cells. MP was able to inhibit the phagocytic function of RAW cells. Treatment of cells with MP 24 hours prior to LPS stimulation significantly decreased nitric oxide release and altered the pattern of cytokines release; there was a significant decrease in TNF-{alpha} and a significant increase in IL-10 compared to the controls. However, there is a non-significant change in IL-6 level. Furthermore, phosphorylation of inhibitory kappa B (I{kappa}B{alpha}) protein was significantly decreased in RAW cells treated with 0.5 mM MP after LPS stimulation. Based upon the in-vitro results, it was examined whether MP treatment will be effective in preventing bleomycin-induced lung inflammation and fibrosis in-vivo. Bleomycin given by itself caused destruction of the lung architecture characterized by pulmonary fibrosis with collapse of air alveoli and emphysematous. Bleomycin induced a significant increase in hydroxyproline level and activated NF-{kappa}B, p65 expression in the lung. MP co-treatment significantly ameliorated bleomycin effects. These results suggest that MP has a potential of inhibiting macrophages in general. The present study demonstrated for the first time that MP has anti-inflammatory and antifibrotic effect that could be through NF-kB inhibition. Thus MP like molecule could be a promising anti-inflammatory and antifibrotic drug. - Research Highlights: >Methyl palmitate is a universal macrophage inhibitor. >It could be a promising nucleus of anti-inflammatory and antifibrotic drugs. >The underlying mechanism of these effects could be through NF-kB inhibition.

  18. Ethanol increases matrix metalloproteinase-12 expression via NADPH oxidase-dependent ROS production in macrophages

    SciTech Connect (OSTI)

    Kim, Mi Jin; Nepal, Saroj; Lee, Eung-Seok; Jeong, Tae Cheon [College of Pharmacy, Yeungnam University, Gyeongsanbuk-do 712-749 (Korea, Republic of); Kim, Sang-Hyun [Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Park, Pil-Hoon, E-mail: parkp@yu.ac.kr [College of Pharmacy, Yeungnam University, Gyeongsanbuk-do 712-749 (Korea, Republic of)

    2013-11-15T23:59:59.000Z

    Matrix metalloproteinase-12 (MMP-12), an enzyme responsible for degradation of extracellular matrix, plays an important role in the progression of various diseases, including inflammation and fibrosis. Although most of those are pathogenic conditions induced by ethanol ingestion, the effect of ethanol on MMP-12 has not been explored. In the present study, we investigated the effect of ethanol on MMP-12 expression and its potential mechanisms in macrophages. Here, we demonstrated that ethanol treatment increased MMP-12 expression in primary murine peritoneal macrophages and RAW 264.7 macrophages at both mRNA and protein levels. Ethanol treatment also significantly increased the activity of nicotinamide adenine dinucleotide (NADPH) oxidase and the expression of NADPH oxidase-2 (Nox2). Pretreatment with an anti-oxidant (N-acetyl cysteine) or a selective inhibitor of NADPH oxidase (diphenyleneiodonium chloride (DPI)) prevented ethanol-induced MMP-12 expression. Furthermore, knockdown of Nox2 by small interfering RNA (siRNA) prevented ethanol-induced ROS production and MMP-12 expression in RAW 264.7 macrophages, indicating a critical role for Nox2 in ethanol-induced intracellular ROS production and MMP-12 expression in macrophages. We also showed that ethanol-induced Nox2 expression was suppressed by transient transfection with dominant negative I?B-? plasmid or pretreatment with Bay 11-7082, a selective inhibitor of NF-?B, in RAW 264.7 macrophages. In addition, ethanol-induced Nox2 expression was also attenuated by treatment with a selective inhibitor of p38 MAPK, suggesting involvement of p38 MAPK/NF-?B pathway in ethanol-induced Nox2 expression. Taken together, these results demonstrate that ethanol treatment elicited increase in MMP-12 expression via increase in ROS production derived from Nox2 in macrophages. - Highlights: Ethanol increases ROS production through up-regulation of Nox2 in macrophages. Enhanced oxidative stress contributes to ethanol-induced MMP-12 expression. p38 MAPK/NF-?B signaling pathway modulates ethanol-induced Nox2 expression.

  19. B-meson spectroscopy in HQET at order 1/m

    E-Print Network [OSTI]

    Bernardoni, Fabio; Bulava, John; Della Morte, Michele; Fritzsch, Patrick; Garron, Nicolas; Grardin, Antoine; Heitger, Jochen; von Hippel, Georg; Simma, Hubert

    2015-01-01T23:59:59.000Z

    We present a study of the B spectrum performed in the framework of Heavy Quark Effective Theory expanded to next-to-leading order in 1/m and non-perturbative in the strong coupling. Our analyses have been performed on Nf=2 lattice gauge field ensembles corresponding to three different lattice spacings and a wide range of pion masses. We obtain the Bs-meson mass and hyperfine splittings of the B- and Bs-mesons that are in good agreement with the experimental values and examine the mass difference m_{Bs}-m_B as a further cross-check of our previous estimate of the b-quark mass. We also report on the mass splitting between the first excited state and the ground state in the B and Bs systems.

  20. A fully-integrated aptamer-based affinity assay platform for monitoring astronaut health in space.

    SciTech Connect (OSTI)

    Yang, Xianbin (AM Biotechnologies LLC, Houston, TX); Durland, Ross H. (AM Biotechnologies LLC, Houston, TX); Hecht, Ariel H.; Singh, Anup K.; Sommer, Gregory Jon; Hatch, Anson V.

    2010-07-01T23:59:59.000Z

    Here we demonstrate the suitability of robust nucleic acid affinity reagents in an integrated point-of-care diagnostic platform for monitoring proteomic biomarkers indicative of astronaut health in spaceflight applications. A model thioaptamer targeting nuclear factor-kappa B (NF-{kappa}B) is evaluated in an on-chip electrophoretic gel-shift assay for human serum. Key steps of (i) mixing sample with the aptamer, (ii) buffer exchange, and (iii) preconcentration of sample were successfully integrated upstream of fluorescence-based detection. Challenges due to (i) nonspecific interactions with serum, and (ii) preconcentration at a nanoporous membrane are discussed and successfully resolved to yield a robust, rapid, and fully-integrated diagnostic system.