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Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

Complete Genome of the Cellulolytic Ruminal Bacterium Ruminococcus albus 7  

SciTech Connect (OSTI)

Ruminococcus albus 7 is a highly cellulolytic ruminal bacterium that is a member of the phylum Firmicutes. Here, we describe the complete genome of this microbe. This genome will be useful for rumen microbiology and cellulosome biology and in biofuel production, as one of its major fermentation products is ethanol.

Suen, Garret [University of Wisconsin, Madison; Stevenson, David M [USDA-ARS, Madison WI; Bruce, David [Los Alamos National Laboratory (LANL); Chertkov, Olga [Los Alamos National Laboratory (LANL); Copeland, A [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Hauser, Loren John [ORNL; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Boyum, Julie [University of Wisconsin, Madison; Mead, David [University of Wisconsin, Madison; Weimer, Paul J [USDA-ARS, Madison WI

2011-01-01T23:59:59.000Z

2

Complete Genome Sequence of the Thermophilic, Piezophilic, Heterotrophic Bacterium Marinitoga piezophila KA3  

SciTech Connect (OSTI)

Marinitoga piezophila KA3 is a thermophilic, anaerobic, chemoorganotrophic, sulfur-reducing bacterium isolated from the Grandbonum deep-sea hydrothermal vent site at the East Pacific Rise (13 degrees N, 2,630-m depth). The genome of M. piezophila KA3 comprises a 2,231,407-bp circular chromosome and a 13,386-bp circular plasmid. This genome was sequenced within Department of Energy Joint Genome Institute CSP 2010.

Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Han, James [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Peters, Lin [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Vannier, Pauline [Ifremer, Plouzane, France; Oger, Phil [University of Lyon, France; Bartlett, Douglas [University of California, San Diego & La Jolla; Noll, Kenneth M [University of Connecticut, Storrs; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Jebbar, Mohamed [Ifremer, Plouzane, France

2012-01-01T23:59:59.000Z

3

Glycoside hydrolase inventory drives plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Glycoside Glycoside Hydrolase Inventory Drives Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Amy L. VanFossen, Inci Ozdemir, Samantha L. Zelin, Robert M. Kelly Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905; telephone: 919-515-6396; fax: 919-515-3465; e-mail: rmkelly@eos.ncsu.edu Received 15 November 2010; revision received 22 January 2011; accepted 1 February 2011 Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/bit.23093 ABSTRACT: The genome of Caldicellulosiruptor saccharoly- ticus encodes a range of glycoside hydrolases (GHs) that mediate plant biomass deconstruction by this bacterium. Two GH-based genomic loci that appear to be central to the hydrolysis of hemicellulosic and cellulosic substrates

4

Complete Genome Sequence of the Thermophilic Bacterium Exiguobacterium sp. AT1b  

SciTech Connect (OSTI)

Here we present the genome of strain Exiguobacterium sp. AT1b, a thermophilic member of the genus Exiguobacterium whose representatives were isolated from various environments along a thermal and physicochemical gradient. This genome was sequenced to be a comparative resource for the study of thermal adaptation with a psychroactive representative of the genus, Exiguobacterium sibiricum strain 255-15, that was previously sequenced by the U.S. Department of Energy s (DOE s) Joint Genome Institute (JGI) (http://genome.ornl.gov/microbial/exig/).

Vishnivetskaya, T. [University of Tennessee, Knoxville (UTK); Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L [ORNL; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Saunders, Elizabeth H [Los Alamos National Laboratory (LANL); Brettin, Tom [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Larimer, Frank W [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Kathariou, Sophia [North Carolina State University; Ramaley, Robert F. [University of Nebraska Medical Center; Rodrigues, Debora F. [University of Houston, Houston; Hendrix, Christie [Yellowstone National Park; Richardson, Paul [U.S. Department of Energy, Joint Genome Institute; Tiedje, James M. [Michigan State University, East Lansing

2011-01-01T23:59:59.000Z

5

Complete Genome Sequence of the Thermophilic Bacterium Exiguobacterium sp. AT1b  

SciTech Connect (OSTI)

Here we present the genome of strain Exiguobacterium sp. AT1b, a thermophilic member of the genus Exiguobacterium whose representatives were isolated from various environments along a thermal and physico-chemical gradient. This genome was sequenced to be a comparative resource for study of thermal adaptation with a psychroactive representative of the genus, Exiguobacterium sibiricum strain 255-15, that was previously sequenced by the U.S. Department of Energy's (DOE) Joint Genome Institute (JGI) (http://genome.ornl.gov/microbial/exig/).

Vishnivetskaya, T. [University of Tennessee, Knoxville (UTK); Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Saunders, Elizabeth H [Los Alamos National Laboratory (LANL); Brettin, Thomas S [ORNL; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Larimer, Frank W [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Kathariou, Sophia [North Carolina State University; Ramaley, Robert F. [University of Nebraska Medical Center; Rodrigues, Debora F. [University of Houston, Houston; Hendrix, Christie [Yellowstone National Park; Richardson, Paul [U.S. Department of Energy, Joint Genome Institute; Tiedje, James M. [Michigan State University, East Lansing

2011-01-01T23:59:59.000Z

6

Complete genome sequence of Thermovibrio ammonificans HB-1T, a thermophilic, chemolithoautotrophic bacterium isolated from a deep-sea hydrothermal vent  

SciTech Connect (OSTI)

Thermovibrio ammonificans type strain HB-1T is a thermophilic (Topt: 75 C), strictly anaero- bic, chemolithoautotrophic bacterium that was isolated from an active, high temperature deep-sea hydrothermal vent on the East Pacific Rise. This organism grows on mineral salts medium in the presence of CO2/H2, using NO3- or S0 as electron acceptors, which are re- duced to ammonium or hydrogen sulfide, respectively. T. ammonificans is one of only three species within the genus Thermovibrio, a member of the family Desulfurobacteriaceae, and it forms a deep branch within the phylum Aquificae. Here we report the main features of the genome of T. ammonificans strain HB-1T (DSM 15698T).

Giovannelli, Donato [Rutgers University; Ricci, Jessica [Rutgers University; Perez-Rodriguez, Ileana [Rutgers University; Hugler, Michael [Woods Hole Oceanographic Institution (WHOI), Woods Hole, MA; O'Brien, Charles [Rutgers University; Keddis, Ramaydalis [Rutgers University; Grosche, Ashley [Rutgers University; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Davenport, Karen W. [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Han, James [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Vetriani, Costantino [Rutgers University

2012-01-01T23:59:59.000Z

7

Genome sequence of the thermophilic sulfate-reducing ocean bacterium Thermodesulfatator indicus type strain (CIR29812T)  

SciTech Connect (OSTI)

Thermodesulfatator indicus Moussard et al. 2004 is a member of the genomically so far poorly characterized family Thermodesulfobacteriaceae in the phylum Thermodesulfobacteria. Members of this phylum are of interest because they represent a distinct, deep-branching, Gram-negative lineage. T. indicus is an anaerobic, thermophilic, chemolithoautotrophic sulfate reducer isolated from a deep-sea hydrothermal vent. Here we describe the features of this organism, together with the complete genome sequence, and annotation. The 2,322,224 bp long chromosome with its 2,233 protein-coding and 58 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Anderson, Iain [U.S. Department of Energy, Joint Genome Institute; Saunders, Elizabeth H [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Chang, Yun-Juan [ORNL; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2012-01-01T23:59:59.000Z

8

Genomics of cellulolytic bacteria  

Science Journals Connector (OSTI)

The heterogeneous plant biomass is efficiently decomposed by the interplay of a great number of different enzymes. The enzyme systems in cellulolytic bacteria have been investigated by sequencing and bioinformatic analysis of genomes from plant biomass degrading microorganisms with valuable insights into the variety of the involved enzymes. This broadened our understanding of the biochemical mechanisms of plant polymer degradation and made the enzymes applicable for modern biotechnology. A list of the truly cellulolytic bacteria described and the available genomic information was examined for proteins with cellulolytic and hemicellulolytic capability. The importance of the isolation, characterization and genomic sequencing of cellulolytic microorganisms and their usage for sustainable energy production from biomass and other residues, is emphasized.

Daniela E Koeck; Alexander Pechtl; Vladimir V Zverlov; Wolfgang H Schwarz

2014-01-01T23:59:59.000Z

9

Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production  

Science Journals Connector (OSTI)

This study investigated expression of a thermophilic amylase (arAmyBH) from hyperthermophilic bacterium Thermotoga neapolitana into Chlamydomonas reinhardtii...chloroplast to facilitate in vivo starch hydrolysis ...

Xiaoqing Wang; Zhenhua Ruan; Danielle Boileau; Barbara B. Sears…

2014-10-01T23:59:59.000Z

10

E-Print Network 3.0 - acid-producing bacterium streptococcus...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Ecology 2 Isolation of plasmids present in thermophilic strains from hot springs in Jordan Amjad B. Khalil1, Summary: acid producing bacterium with an optimum growth temperature...

11

Picosecond Energy Transfer Kinetics between Different Pigment Pools in Chlorosomes from the Green Bacterium Chloroflexus Aurantiacus  

Science Journals Connector (OSTI)

Chloroflexus aurantiacus a thermophilic green bacterium, contains at least four different bacteriochlorophyll ... coupled in a specific way to optimize the energy transfer from the main antenna, ...

Kai Griebenow; Marc G. Müller…

1990-01-01T23:59:59.000Z

12

Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov.  

SciTech Connect (OSTI)

A thermophilic, filamentous, heterotrophic bacterium designated strain JAD2T was isolated from sediment of Great Boiling Spring in Nevada, USA. Cells had an average diameter of 0.3 µm and length of 4.0 µm, and formed filaments typically ranging in length from 20 µm to 200 µm. Filaments were negative for the Gram stain reaction, spores were not formed, and motility was not observed. The optimum temperature for growth was 75 °C with a range from 67.5-75 °C, and the optimum pH for growth was 6.75 with a range from 6.5-7.75. Peptone, tryptone or yeast extract were able to support growth when supplemented with a vitamin solution, but no growth was observed using a variety of defined organic substrates. Strain JAD2T was a facultative microaerophile, with optimal growth at 1% v/v O2 and an upper limit of 8% O2, and anaerobic growth was stimulated by fumarate but inhibited by sulfite and elemental sulfur. The major cellular fatty acids (>5%) were C16:0, C19:0, C18:0, C20:0, and C19:1. The genomic DNA G+C content was 69.3%. Phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and other conserved genes placed JAD2T and other members of the yet-uncultivated GAL35 group within the phylum Chloroflexi, but not within any existing class in this phylum. These results indicate that strain JAD2T is the first cultivated representative of a new lineage within the phylum Chloroflexi, for which we propose the name Thermoflexus hugenholtzii gen. nov., sp. nov., type strain JAD2T, within Thermoflexia classis nov., Thermoflexales ord. nov., and Thermoflexaceae fam. nov.

Dodsworth, Jeremy A.; Gevorkian, Jonathan; Despujos, Fairuz; Cole, Jesse; Murugapiran, Senthil K.; Ming, Hong; Li, Wen J.; Zhang, Gengxin; Dohnalkova, Alice; Hedlund, Brian P.

2014-06-06T23:59:59.000Z

13

Pigment organization and energy transfer in the green photosynthetic bacterium Chloroflexus aurantiacus. III. Energy transfer in whole cells  

Science Journals Connector (OSTI)

The transfer of excitation energy in intact cells of the thermophilic green photosynthetic bacterium Chloroflexus aurantiacus was studied both at low temperature and under more physiological conditions. Analysis ...

Rob J. van Dorssen; Jan Amesz

1988-02-01T23:59:59.000Z

14

Polypeptide from a cellulolytic fungus having cellulolytic enhancing activity  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Brown, Kimberly (Elk Grove, CA); Harris, Paul (Carnation, WA); Zaretsky, Elizabeth (Reno, NV); Re, Edward (Davis, CA); Vlasenko, Elena (Davis, CA); McFarland, Keith (Davis, CA); Lopez de Leon, Alfredo (Davis, CA)

2008-04-22T23:59:59.000Z

15

Genome Sequence of Kosmotoga olearia Strain TBF 19.5.1, a Thermophilic Bacterium with a Wide Growth Temperature Range, Isolated from the Troll B Oil Platform in the North Sea  

SciTech Connect (OSTI)

Kosmotoga olearia strain TBF 19.5.1 is a member of the Thermotogales that grows best at 65 degrees C and very well even at 37 degrees C. Information about this organism is important for understanding the evolution of mesophiles from thermophiles. Its genome sequence reveals extensive gene gains and a large content of mobile genetic elements. It also contains putative hydrogenase genes that have no homologs in the other member of the Thermotogales.

Swithers, Kristen S [University of Connecticut, Storrs; DiPippo, Jonathan L [University of Connecticut, Storrs; Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Nesbo, Camilla [University of Oslo, Norway; Gogarten, Peter [University of Connecticut, Storrs; Noll, Kenneth M [University of Connecticut, Storrs

2011-01-01T23:59:59.000Z

16

Activity-based protein profiling of secreted cellulolytic enzyme...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30 Activity-based protein profiling of secreted cellulolytic...

17

Complete Genome Sequence of the Cellulose-Degrading Bacterium Cellulosilyticum lentocellum  

SciTech Connect (OSTI)

Cellulosilyticum lentocellum DSM 5427 is an anaerobic, endospore-forming member of the Firmicutes. We describe the complete genome sequence of this cellulose-degrading bacterium; originally isolated from estuarine sediment of a river that received both domestic and paper mill waste. Comparative genomics of cellulolytic clostridia will provide insight into factors that influence degradation rates.

Miller, David A [Cornell University; Suen, Garret [University of Wisconsin, Madison; Bruce, David [Los Alamos National Laboratory (LANL); Copeland, A [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Hauser, Loren John [ORNL; Land, Miriam L [ORNL; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Meincke, Linda [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Teshima, Hazuki [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Fox, Brian G. [University of Wisconsin, Madison; Angert, Esther R. [Cornell University; Currie, Cameron [University of Wisconsin, Madison

2011-01-01T23:59:59.000Z

18

THE THERMOPHILIC MICROÖRGANISMS  

Science Journals Connector (OSTI)

...apparently in the maximum stationary phase of the cultural cycle. In...rates. The logarithmic growth phase, during which the rate of...enzymes in the medium. The behavior of amylolytic thermophiles...paralleled population data. The behavior of the stenothermophilic bacteria...

Eugene R. L. Gaughran

1947-09-01T23:59:59.000Z

19

Anaerobic thermophilic culture system  

DOE Patents [OSTI]

A mixed culture system of the newly discovered microorganism Thermoanaerobacter ethanolicus ATCC31550 and the microorganism Clostridium thermocellum ATCC31549 is described. In a mixed nutrient culture medium that contains cellulose, these microorganisms have been coupled and cultivated to efficiently ferment cellulose to produce recoverable quantities of ethanol under anaerobic, thermophilic conditions.

Ljungdahl, Lars G. (Athens, GA); Wiegel, Jurgen K. W. (Gottingen, DE)

1981-01-01T23:59:59.000Z

20

Why Sequence Thermophilic Methanoarchaea?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Thermophilic Methanoarchaea? Thermophilic Methanoarchaea? Microbes that reduce carbon dioxide to methane catalyze a significant component of the carbon cycle on Earth and are responsible for most of the methane in the atmospere. Since methane contributes significantly to the greenhouse effect, understanding the biological controls on emissions of methane may provide the opportunity to compensate for the emission of other important trace gases, such as CO2. In addition, methane is a common fuel, and the biological production of methane catalyzed by these bacteria has great potential as an alternative fuel source. This application is of special significance because it is "CO2 neutral", ie., it does not result in the net emissions of greenhouse gases to the earth's atmosphere.

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus  

Science Journals Connector (OSTI)

...transition to a hydrogen economy-to 2030 and beyond. United States Department of Energy, Washington, DC. http://www1.eere.energy.gov/hydrogenandfuelcells/pdfs/vision_doc.pdf . 48 van Niel, E. W. J., M. A. W. Budde, G. G. de...

Harmen J. G. van de Werken; Marcel R. A. Verhaart; Amy L. VanFossen; Karin Willquist; Derrick L. Lewis; Jason D. Nichols; Heleen P. Goorissen; Emmanuel F. Mongodin; Karen E. Nelson; Ed W. J. van Niel; Alfons J. M. Stams; Donald E. Ward; Willem M. de Vos; John van der Oost; Robert M. Kelly; Servé W. M. Kengen

2008-09-05T23:59:59.000Z

22

Hydrogenomics of the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus  

Science Journals Connector (OSTI)

...utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing...utilize a range of carbohydrates associated with biomass feedstocks is a highly desirable feature of this lignocellulose-utilizing...

Harmen J. G. van de Werken; Marcel R. A. Verhaart; Amy L. VanFossen; Karin Willquist; Derrick L. Lewis; Jason D. Nichols; Heleen P. Goorissen; Emmanuel F. Mongodin; Karen E. Nelson; Ed W. J. van Niel; Alfons J. M. Stams; Donald E. Ward; Willem M. de Vos; John van der Oost; Robert M. Kelly; Servé W. M. Kengen

2008-09-05T23:59:59.000Z

23

Complete Genome Sequence of the Cellulolytic Thermophile Clostridium thermocellum DSM1313  

Science Journals Connector (OSTI)

...Davenport 2 David Bruce 2 Chris Detter 3 Roxanne Tapia 2 Cliff Han 2 Alla Lapidus 4 Susan Lucas 3 Jan-Fang Cheng 3 Samuel Pitluck 3 Tanja Woyke 3 Natalia Ivanova 3 Natalia Mikhailova 3 Miriam Land 5 Loren Hauser 5 D. Aaron Argyros 1 Lynne Goodwin 2 David...

Lawrence Feinberg; Justine Foden; Trisha Barrett; Karen Walston Davenport; David Bruce; Chris Detter; Roxanne Tapia; Cliff Han; Alla Lapidus; Susan Lucas; Jan-Fang Cheng; Samuel Pitluck; Tanja Woyke; Natalia Ivanova; Natalia Mikhailova; Miriam Land; Loren Hauser; D. Aaron Argyros; Lynne Goodwin; David Hogsett; Nicky Caiazza

2011-04-01T23:59:59.000Z

24

Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47T  

Science Journals Connector (OSTI)

...Laboratory, Los Alamos, New Mexico 87545 4 DOE Joint Genome...the primary carbon and energy source for cultivation...U.S. Department of Energy (DOE) Joint Genome...different aspects of renewable energy production, genome-enabled...

James G. Elkins; Adriane Lochner; Scott D. Hamilton-Brehm; Karen Walston Davenport; Mircea Podar; Steven D. Brown; Miriam L. Land; Loren J. Hauser; Dawn M. Klingeman; Babu Raman; Lynne A. Goodwin; Roxanne Tapia; Linda J. Meincke; J. Chris Detter; David C. Bruce; Cliff S. Han; Anthony V. Palumbo; Robert W. Cottingham; Martin Keller; David E. Graham

2010-09-17T23:59:59.000Z

25

Characterization of the cellulolytic activity of a bacillus isolate  

SciTech Connect (OSTI)

A group I Bacillus strain, DLG, was isolated and characterized as being most closely related to Bacillus subtilis. When grown on any of a variety of sugars, the culture supernatant of this isolate was found to process cellulolytic activity, as demonstrated by degradation of trinitrophenyl-carboxymethyl cellulose. Growth in medium containing cellobiose or glucose resulted in the greatest production of cellulolytic activity. The cellulolytic activity was not produced until the stationary phase of growth, and the addition of glucose or cellobiose to a culture in this phase had no apparent effect on enzyme production. Fractionation of the culture supernatant showed that the molecular weight of the enzymatic activity was less than 100,000. Maximum cellulolytic activity in assays was observed at pH 4.8 and at 58 degrees C, although maximum thermal stability of the activity was observed only up to 45 to 50 degrees C. Neither glucose nor cellobiose inhibited enzymatic activity. Kinetic experiments suggested that more than one enzyme was acting upon trinitrophenyl-carboxymethyl cellulose. Exocellular protein produced by this Bacillus isolate showed roughly one-fifth the cellulolytic activity displayed by Trichoderma reesei C30 on noncrystalline cellulosic substrates. In contrast to T. reesei cellulase, the Bacillus enzymatic activity showed no ability to degrade crystalline forms of cellulose, nor was cellobiase activity detectable. 32 references.

Robson, L.M.; Chambliss, G.H.

1984-05-01T23:59:59.000Z

26

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Maiyuran, Suchindra; Kramer, Randall; Harris, Paul

2013-10-29T23:59:59.000Z

27

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Maiyuran, Suchindra; Kramer, Randall; Harris, Paul

2014-10-21T23:59:59.000Z

28

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Duan, Junxin (Beijing, CN); Liu, Ye (Beijing, CN); Tang, Lan (Beijing, CN); Wu, Wenping (Beijing, CN); Quinlan, Jason (Albany, CA); Kramer, Randall (Lincoln, CA)

2012-03-27T23:59:59.000Z

29

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding the same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Tang, Lan; Liu, Ye; Duan, Junxin; Wu, Wenping; Kramer, Randall

2013-11-19T23:59:59.000Z

30

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Tang, Lan; Liu, Ye; Duan, Junxin; Zhang, Yu; Joergensen, Christian; Kramer, Randall

2014-09-16T23:59:59.000Z

31

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Tang, Lan (Beijing, CN); Liu, Ye (Beijing, CN); Duan, Junxin (Beijing, CN); Zhang, Yu (Beijing, CN); Jorgensen, Christian Isak (Bagsvaerd, DK); Kramer, Randall (Lincoln, CA)

2012-04-03T23:59:59.000Z

32

Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Tang, Lan; Liu, Ye; Duan, Junxin; Zhang, Yu; Jorgensen, Christian Isak; Kramer, Randall

2013-04-16T23:59:59.000Z

33

Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same  

DOE Patents [OSTI]

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Brown, Kimberly; Harris, Paul; Zaretsky, Elizabeth; Re, Edward; Vlasenko, Elena; McFarland, Keith; Lopez de Leon, Alfredo

2014-09-30T23:59:59.000Z

34

THE THERMOPHILIC AEROBIC SPOREFORMING BACTERIA  

Science Journals Connector (OSTI)

...oxygen was clearly shown by the behavior of this strain; its growth...similar during the logarithmic phase but deviated when growth became...fettsaurer Salze und Kohlen- hydrate durch thermophile Bakterien...127. NwsRA, T. 1897 On the behavior of yeast at a high temperature...

Mary Belle Allen

1953-06-01T23:59:59.000Z

35

Biocorrosive Thermophilic Microbial Communities in Alaskan North Slope Oil Facilities  

E-Print Network [OSTI]

anaerobic thermophilic oil reservoir and well communities.been detected in hot oil reservoirs and production fluids (other thermophilic oil reservoirs and wells suggests that

Duncan, Kathleen E.

2010-01-01T23:59:59.000Z

36

Direct in Situ Observation of Synergism between Cellulolytic Enzymes during the Biodegradation of Crystalline Cellulose Fibers  

E-Print Network [OSTI]

Direct in Situ Observation of Synergism between Cellulolytic Enzymes during the Biodegradation types of T. reesei cellulolytic enzymes TrCel6A, TrCel7A, and TrCel7Band their mixtures. TrCel6A and Tr. When acting alone on native cellulose fibers, each of the three enzymes is incapable of significant

Dutcher, John

37

Compositions for enhancing hydroysis of cellulosic material by cellulolytic enzyme compositions  

DOE Patents [OSTI]

The present invention relates to compositions comprising a GH61 polypeptide having cellulolytic enhancing activity and an organic compound comprising a carboxylic acid moiety, a lactone moiety, a phenolic moiety, a flavonoid moiety, or a combination thereof, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the organic compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme compared to the GH61 polypeptide alone or the organic compound alone. The present invention also relates to methods of using the compositions.

Quinlan, Jason; Xu, Feng; Sweeney, Matthew; Johansen, Katja Salomon

2014-09-30T23:59:59.000Z

38

Why sequence carbon monoxide oxidizing thermophiles?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

carbon monoxide oxidizing thermophiles? carbon monoxide oxidizing thermophiles? Many microbes that use carbon monoxide as an energy source are found in high temperature environments such as geothermal areas. Researchers think that these carboxydotrophs may be involved in reducing potentially toxic carbon monoxide hotspots by combine with water to form hydrogen, carbon dioxide and acetate, which are in turn used for thermophilic energy conservation and carbon sequestration mechanisms. The project focuses on sequencing two closely related microbes, one of which is Carboxydothermus hydrogenformans. A strain of C. hydrogenformans has been grown in hydrogen-enriched synthesis gas (syngas), which contains a mix of hydrogen and carbon monoxide. Researchers are interested in sequencing both microbial strains to track the genome's evolution and

39

Thermophilic Fungi: Their Physiology and Enzymes  

Science Journals Connector (OSTI)

...as the primary mechanism in thermal adaptation, eukaryotic thermophily...fungi may be good sources of a battery of purified enzymes that are...enzymes vis-a-vis their thermal stability. Some thermophilic...figured prominently in the development of biochemistry-suggests...

Ramesh Maheshwari; Girish Bharadwaj; Mahalingeshwara K. Bhat

2000-09-01T23:59:59.000Z

40

Complete Genome Sequence of the Filamentous Anoxygenic Phototrophic Bacterium Chloroflexus aurantiacus  

SciTech Connect (OSTI)

Chloroflexus aurantiacus is a thermophilic filamentous anoxygenic phototrophic (FAP) bacterium, and can grow phototrophically under anaerobic conditions or chemotrophically under aerobic and dark conditions. According to 16S rRNA analysis, Chloroflexi species are the earliest branching bacteria capable of photosynthesis, and Cfl. aurantiacus has been long regarded as a key organism to resolve the obscurity of the origin and early evolution of photosynthesis. Cfl. aurantiacus contains a chimeric photosystem that comprises some characters of green sulfur bacteria and purple photosynthetic bacteria, and also has some unique electron transport proteins compared to other photosynthetic bacteria.

Tang, Kuo-Hsiang [Washington University, St. Louis; Barry, Kerrie [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Hauser, Loren John [ORNL; Honchak, Barbara M [Washington University, St. Louis; Karbach, Lauren E [Washington University, St. Louis; Land, Miriam L [ORNL; Lapidus, Alla L. [Joint Genome Institute, Walnut Creek, California; Larimer, Frank W [ORNL; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [Joint Genome Institute, Walnut Creek, California; Pierson, Beverly K [University of Puget Sound, Tacoma, WA

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Use of pectino-cellulolytic enzymes for improving extrac-tion of phloem-limited plant viruses as exemplified by  

E-Print Network [OSTI]

Use of pectino-cellulolytic enzymes for improving extrac- tion of phloem-limited plant viruses-persistent manner ; it has been successfully purified using pectino-cellulolytic enzymes. The enzymes (driselaseH 6 along with enzyme or a mixture of enzymes and incubated with shaking. The filtrate was adjusted

Paris-Sud XI, Université de

42

Parameter estimation for models of ligninolytic and cellulolytic enzyme kinetics  

SciTech Connect (OSTI)

While soil enzymes have been explicitly included in the soil organic carbon (SOC) decomposition models, there is a serious lack of suitable data for model parameterization. This study provides well-documented enzymatic parameters for application in enzyme-driven SOC decomposition models from a compilation and analysis of published measurements. In particular, we developed appropriate kinetic parameters for five typical ligninolytic and cellulolytic enzymes ( -glucosidase, cellobiohydrolase, endo-glucanase, peroxidase, and phenol oxidase). The kinetic parameters included the maximum specific enzyme activity (Vmax) and half-saturation constant (Km) in the Michaelis-Menten equation. The activation energy (Ea) and the pH optimum and sensitivity (pHopt and pHsen) were also analyzed. pHsen was estimated by fitting an exponential-quadratic function. The Vmax values, often presented in different units under various measurement conditions, were converted into the same units at a reference temperature (20 C) and pHopt. Major conclusions are: (i) Both Vmax and Km were log-normal distributed, with no significant difference in Vmax exhibited between enzymes originating from bacteria or fungi. (ii) No significant difference in Vmax was found between cellulases and ligninases; however, there was significant difference in Km between them. (iii) Ligninases had higher Ea values and lower pHopt than cellulases; average ratio of pHsen to pHopt ranged 0.3 0.4 for the five enzymes, which means that an increase or decrease of 1.1 1.7 pH units from pHopt would reduce Vmax by 50%. (iv) Our analysis indicated that the Vmax values from lab measurements with purified enzymes were 1 2 orders of magnitude higher than those for use in SOC decomposition models under field conditions.

Wang, Gangsheng [ORNL; Post, Wilfred M [ORNL; Mayes, Melanie [ORNL; Frerichs, Joshua T [ORNL; Jagadamma, Sindhu [ORNL

2012-01-01T23:59:59.000Z

43

Purification and Reconstitution of the Glutamate Carrier GltT of the Thermophilic Bacterium Bacillus stearothermophilus  

Science Journals Connector (OSTI)

Department of Microbiology, Groningen Biotechnology and Biomolecular Sciences Institute, University of Groningen, The Netherlands ... Ni2+-NTA resin was obtained from QIAGEN and l-[14C]glutamate from Amersham, U.K.; synthetic oligonucleotides were obtained from Eurosequence, Groningen, The Netherlands. ...

Isabelle Gaillard; Dirk-Jan Slotboom; Jan Knol; Juke S. Lolkema; Wil N. Konings

1996-05-14T23:59:59.000Z

44

Prospecting for cellulolytic activity in insect digestive fluids Cris Oppert a  

E-Print Network [OSTI]

purification and make lignocellulosic biofuels a cost-effective alternative to fossil fuels. Despite the large, University of Tennessee, Knoxville, TN 37996, USA c USDA-ARS Grain Marketing & Production Research Center, cellulolytic activity levels in most head fluids were greater on the MCC substrate. Our data suggests

Jurat-Fuentes, Juan Luis

45

Caminibacter mediatlanticus sp. nov., a thermophilic, chemolithoautotrophic,  

E-Print Network [OSTI]

-ammonifying bacterium isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge James W. Voordeckers,1 strain TB-2T , was isolated from the walls of an active deep-sea hydrothermal vent chimney on the Mid. Growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon

Vetriani, Costantino

46

Characterization of cellulolytic activity from digestive fluids of Dissosteira carolina (Orthoptera: Acrididae)  

E-Print Network [OSTI]

for production of ethanol biofuel as an alternative to fossil fuels (Lynd et al., 1991; Wyman, 1999). The main-ARS Center for Grain and Animal Health Research, Manhattan, KS 66502, USA a b s t r a c ta r t i c l e i n f for the gut-derived protein. Our data demonstrate the presence of cellulolytic activity in the digestive

Jurat-Fuentes, Juan Luis

47

Extreme thermophiles: moving beyond single-enzyme biocatalysis  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

COCHE-51; NO. OF PAGES 10 COCHE-51; NO. OF PAGES 10 Please cite this article in press as: Frock AD, Kelly RM. Extreme thermophiles: moving beyond single-enzyme biocatalysis, Curr Opin Chem Eng (2012), http://dx.doi.org/10.1016/ j.coche.2012.07.003 Available online at www.sciencedirect.com Extreme thermophiles: moving beyond single-enzyme biocatalysis Andrew D Frock and Robert M Kelly Extremely thermophilic microorganisms have been sources of thermostable and thermoactive enzymes for over 30 years. However, information and insights gained from genome sequences, in conjunction with new tools for molecular genetics, have opened up exciting new possibilities for biotechnological opportunities based on extreme thermophiles that go beyond single-step biotransformations. Although the pace for discovering novel microorganisms has slowed over

48

Why sequence thermophiles in Great Basin hot springs?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

thermophiles in Great Basin hot springs? thermophiles in Great Basin hot springs? A thermophile is an organism that thrives in extremely hot temperature conditions. These conditions are found in the Great Basin hot springs, where the organisms have been exposed to unique conditions which guide their lifecycle. High temperature environments often support large and diverse populations of microorganisms, which appear to be hot spots of biological innovation of carbon fixation. Sequencing these microbes that make their home in deadly heat could provide various insights into understanding energy production and carbon cycling. Converting cellulosic biomass to ethanol is one of the most promising strategies to reduce petroleum consumption in the near future. This can only be achieved by enhancing recovery of fermentable sugars from complex

49

Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them  

DOE Patents [OSTI]

The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

Gray, Kevin A. (San Diego, CA); Zhao, Lishan (Emeryville, CA); Cayouette, Michelle H. (San Diego, CA)

2012-01-24T23:59:59.000Z

50

Impact of Cell Wall Acetylation on Corn Stover Hydrolysis by Cellulolytic and Xylanolytic Enzymes  

SciTech Connect (OSTI)

Analysis of variously pretreated corn stover samples showed neutral to mildly acidic pretreatments were more effective at removing xylan from corn stover and more likely to maintain the acetyl to xylopyranosyl ratios present in untreated material than were alkaline treatments. Retention of acetyl groups in the residual solids resulted in greater resistance to hydrolysis by endoxylanase alone, although the synergistic combination of endoxylanase and acetyl xylan esterase enzymes permitted higher xylan conversions to be observed. Acetyl xylan esterase alone did little to improve hydrolysis by cellulolytic enzymes, although a direct relationship was observed between the enzymatic removal of acetyl groups and improvements in the enzymatic conversion of xylan present in substrates. In all cases, effective xylan conversions were found to significantly improve glucan conversions achievable by cellulolytic enzymes. Additionally, acetyl and xylan removal not only enhanced the respective initial rates of xylan and glucan conversion, but also the overall extents of conversion. This work emphasizes the necessity for xylanolytic enzymes during saccharification processes and specifically for the optimization of acetyl esterase and xylanase synergies when biomass processes include milder pretreatments, such as hot water or sulfite steam explosion.

Selig, M. J.; Adney, W. S.; Himmel, M. E.; Decker, S. R.

2009-01-01T23:59:59.000Z

51

Complete Genome Sequence of Desulfurococcus fermentans, a Hyperthermophilic Cellulolytic Crenarchaeon Isolated from a Freshwater Hot Spring in Kamchatka, Russia  

SciTech Connect (OSTI)

Desulfurococcus fermentans is the first known cellulolytic archaeon. This hyperthermophilic and strictly anaerobic crenarchaeon produces hydrogen from fermentation of various carbohydrates and peptides without inhibition by accumulating hydrogen. The complete genome sequence reported here suggested that D. fermentans employs membrane-bound hydrogenases and novel glycohydrolases for hydrogen production from cellulose.

Susanti, Dwi [Virginia Polytechnic Institute and State University (Virginia Tech); Johnson, Eric F [Virginia Polytechnic Institute and State University (Virginia Tech); Rodriquez, Jason [Virginia Polytechnic Institute and State University (Virginia Tech); Anderson, Iain [U.S. Department of Energy, Joint Genome Institute; Perevalova, Anna [Virginia Polytechnic Institute and State University (Virginia Tech); Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Han, James [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Peters, Lin [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Gopapan, Venkay [Ohio State University; Chan, Patricia [University of California, Santa Cruz; Atomi, Haruyuki [Kyoto University, Japan; Bonch-Osmolovskaya, Elizaveta [Russian Academy of Sciences, Moscow; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Mukhopadhyay, Biswarup [Virginia Polytechnic Institute and State University (Virginia Tech)

2012-01-01T23:59:59.000Z

52

Onestep production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

One-step One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges One-step consolidated bioprocessing that integrates cellulase production, cellulose hydrolysis, and product fermentation into a single step for decreasing costly cellulase use, increasing volumetric productivity, and reducing capital investment is widely accepted for low-cost production of biofuels or other value-added biochemicals. Considering the narrow margins between biomass and low-value biocommodities, good physiological performance of industrial microbes is crucial for economically viable production. Bacillus subtilis, the best-characterized Gram-positive microorganism, is a major industrial microorganism with numerous valuable features such as hexose and pentose utilization, low-nutrient needs,

53

Development and Evaluation of Methods to Infer Biosynthesis and Substrate Consumption in Cultures of Cellulolytic Microorganisms  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and and Evaluation of Methods to Infer Biosynthesis and Substrate Consumption in Cultures of Cellulolytic Microorganisms Evert K. Holwerda, Lucas D. Ellis, Lee R. Lynd Thayer School of Engineering at Dartmouth College, 14 Engineering Drive, Hanover, New Hampshire, 03755; telephone: 1-6036462231; fax: 1-6036462277; e-mail: lee.r.lynd@dartmouth.edu ABSTRACT: Concentrations of biosynthate (microbial bio- mass plus extracellular proteins) and residual substrate were inferred using elemental analysis for batch cultures of Clostridium thermocellum. Inferring residual substrate based on elemental analysis for a cellulose (Avicel)-grown culture shows similar results to residual substrate determined by quantitative saccharification using acid hydrolysis. Inference based on elemental analysis is also compared to different on- line measurements: base addition, CO

54

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb.  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Feb. Feb. 2010, p. 1014-1020 Vol. 76, No. 4 0099-2240/10/$12.00 doi:10.1128/AEM.01903-09 Copyright © 2010, American Society for Microbiology. All Rights Reserved. Caldicellulosiruptor obsidiansis sp. nov., an Anaerobic, Extremely Thermophilic, Cellulolytic Bacterium Isolated from Obsidian Pool, Yellowstone National Park ᰔ Scott D. Hamilton-Brehm, Jennifer J. Mosher, Tatiana Vishnivetskaya, Mircea Podar, Sue Carroll, Steve Allman, Tommy J. Phelps, Martin Keller, and James G. Elkins* BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 Received 7 August 2009/Accepted 2 December 2009 A novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47 T , was isolated from Obsidian Pool, Yellowstone National Park, WY. The isolate was a nonmotile, non-spore-forming, Gram- positive

55

Complete genome sequence of the thermophilic sulfur-reducer Desulfurobacterium thermolithotrophum type strain (BSAT) from a deep-sea hydrothermal vent  

SciTech Connect (OSTI)

Desulfurobacterium thermolithotrophum L'Haridon et al. 1998 is the type species of the ge- nus Desulfurobacterium which belongs to the family Desulfurobacteriaceae. The species is of interest because it represents the first thermophilic bacterium that can act as a primary pro- ducer in the temperature range of 45-75 C (optimum 70 C) and is incapable of growing un- der microaerophilic conditions. Strain BSAT preferentially synthesizes high-melting-point fatty acids (C18 and C20) which is hypothesized to be a strategy to ensure the functionality of the membrane at high growth temperatures. This is the second completed genome sequence of a member of the family Desulfurobacteriaceae and the first sequence from the genus Desulfu- robacterium. The 1,541,968 bp long genome harbors 1,543 protein-coding and 51 RNA genes and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Daligault, Hajnalka E. [Los Alamos National Laboratory (LANL); Mwirichia, Romano [Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Pan, Chongle [ORNL; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Sikorski, Johannes [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Wirth, Reinhard [Universitat Regensburg, Regensburg, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2011-01-01T23:59:59.000Z

56

E-Print Network 3.0 - anaerobic thermophilic biogas Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

biogas Search Powered by Explorit Topic List Advanced Search Sample search results for: anaerobic thermophilic biogas Page: << < 1 2 3 4 5 > >> 1 Institute for Renewable Energy Ltd...

57

E-Print Network 3.0 - anaerobic thermophilic bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

MICROBIOLOGY, Apr. 1994, p. 1241-1248 0099-22409404.00+0 Summary: . 1991. Acetate oxidation in a thermophilic anaerobic sewage-sludge digestor: the importance of non... -...

58

E-Print Network 3.0 - aerobic co-oxidizing thermophile Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Life LUCA may be a Thermophile Possibly more complex than some existing life 12;The Tree of Life 12... ) Alkalophiles - inhabit alkaline lakes (pH10) Barophiles -...

59

The adhesion force study of dairy thermophile Anoxybacillus flavithermus CM with atomic force microscopy.  

E-Print Network [OSTI]

??Anoxybacillus flavithermus is a common species of thermophilic bacteria discovered in most milk powder manufacturing plants through out New Zealand. The contamination of it’s spores… (more)

Mohd Saidi, Mohd Salihin

2014-01-01T23:59:59.000Z

60

Nautilia abyssi sp. nov.,1 a novel thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific2  

E-Print Network [OSTI]

isolated from an East Pacific2 Rise hydrothermal vent3 4 Karine Alain1 , Nolwenn Callac1 , Marianne Guégan1 Rise hydrothermal vent (13°N) sample and subjected to a polyphasic27 taxonomic analysis. The cells were° as an electron acceptor and CO2 as a31 carbon source. Alternatively, strain PH1209T was able to use peptone

Boyer, Edmond

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris  

SciTech Connect (OSTI)

Thermostable enzymes and thermophilic cell factories may afford economic advantages in the production of many chemicals and biomass-based fuels. Here we describe and compare the genomes of two thermophilic fungi, Myceliophthora thermophila and Thielavia terrestris. To our knowledge, these genomes are the first described for thermophilic eukaryotes and the first complete telomere-to-telomere genomes for filamentous fungi. Genome analyses and experimental data suggest that both thermophiles are capable of hydrolyzing all major polysaccharides found in biomass. Examination of transcriptome data and secreted proteins suggests that the two fungi use shared approaches in the hydrolysis of cellulose and xylan but distinct mechanisms in pectin degradation. Characterization of the biomass-hydrolyzing activity of recombinant enzymes suggests that these organisms are highly efficient in biomass decomposition at both moderate and high temperatures. Furthermore, we present evidence suggesting that aside from representing a potential reservoir of thermostable enzymes, thermophilic fungi are amenable to manipulation using classical and molecular genetics.

Berka, Randy M.; Grigoriev, Igor V.; Otillar, Robert; Salamov, Asaf; Grimwood, Jane; Reid, Ian; Ishmael, Nadeeza; John, Tricia; Darmond, Corinne; Moisan, Marie-Claude; Henrissat, Bernard; Coutinho, Pedro M.; Lombard, Vincent; Natvig, Donald O.; Lindquist, Erika; Schmutz, Jeremy; Lucas, Susan; Harris, Paul; Powlowski, Justin; Bellemare, Annie; Taylor, David; Butler, Gregory; de Vries, Ronald P.; Allijn, Iris E.; van den Brink, Joost; Ushinsky, Sophia; Storms, Reginald; Powell, Amy J.; Paulsen, Ian T.; Elbourne, Liam D. H.; Baker, Scott. E.; Magnuson, Jon; LaBoissiere, Sylvie; Clutterbuck, A. John; Martinez, Diego; Wogulis, Mark; Lopez de Leon, Alfredo; Rey, Michael W.; Tsang, Adrian

2011-05-16T23:59:59.000Z

62

First Knot Discovered in Ancient Bacterium Protein  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

First Knot Discovered in Ancient Bacterium Protein First Knot Discovered in Ancient Bacterium Protein The first knotted protein from the most ancient type of single-celled organism, an archaebacterium, has been discovered by researchers from Argonne National Laboratory and the University of Toronto using the Advanced Photon Source (APS) at Argonne. It is one of the few times that a knot has been seen in any protein structure. Protein folding theory previously held that forming a knot was beyond the ability of a protein. Image of knotted protein. The newly discovered knotted protein comes from a microorganism called Methanobacterium thermoautotrophicum. This organism is of interest to industry for its ability to break down waste products and produce methane gas. Scientists know which gene codes for the 268-amino acid protein, but

63

E-Print Network 3.0 - alkalophilic thermophilic bacillus Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Medicine ; Biotechnology 14 Life on Earth. II The Archean Era Summary: of Life The LCA may be a Thermophile 12;The Tree of Life 12;The Archaea Archaea often inhabit...

64

E-Print Network 3.0 - autoheated thermophilic aerobic Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collection: Renewable Energy 29 Life on Earth. II The Archean Era Summary: of Life The LCA may be a Thermophile 12;The Tree of Life 12;The Archaea Archaea often inhabit...

65

E-Print Network 3.0 - anaerobic extreme thermophilic Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collection: Renewable Energy 22 Life on Earth. II The Archean Era Summary: of Life The LCA may be a Thermophile 12;The Tree of Life 12;The Archaea Archaea often inhabit...

66

E-Print Network 3.0 - acid-tolerant thermophilic bacteria Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and other micro-organisms 12;Implications Life formed very early... of Life The LCA may be a Thermophile 12;The Tree of Life 12;The Archaea Archaea often ... Source:...

67

Start-up of a thermophilic upflow anaerobic sludge bed (UASB) reactor with mesophilic granular sludge  

Science Journals Connector (OSTI)

Fast start-up of thermophilic upflow anaerobic sludge bed (UASB) reactors was achieved at ... 55 and 64° C, using mesophilic granular sludge as inoculum and fatty acid mixtures as ... temperature optima for aceto...

Jules B. van Lier; Katja C. F. Grolle…

1992-04-01T23:59:59.000Z

68

Pathway engineering to improve ethanol production by thermophilic bacteria  

SciTech Connect (OSTI)

Continuation of a research project jointly funded by the NSF and DOE is proposed. The primary project goal is to develop and characterize strains of C. thermocellum and C. thermosaccharolyticum having ethanol selectivity similar to more convenient ethanol-producing organisms. An additional goal is to document the maximum concentration of ethanol that can be produced by thermophiles. These goals build on results from the previous project, including development of most of the genetic tools required for pathway engineering in the target organisms. As well, we demonstrated that the tolerance of C. thermosaccharolyticum to added ethanol is sufficiently high to allow practical utilization should similar tolerance to produced ethanol be demonstrated, and that inhibition by neutralizing agents may explain the limited concentrations of ethanol produced in studies to date. Task 1 involves optimization of electrotransformation, using either modified conditions or alternative plasmids to improve upon the low but reproducible transformation, frequencies we have obtained thus far.

Lynd, L.R.

1998-12-31T23:59:59.000Z

69

Isolation, Identification, and Characterization of a Feather-Degrading Bacterium  

Science Journals Connector (OSTI)

...steam-sterilized or native wool keratin. Molyneaux con- ducted...S. 1959. The digestion of wool by a keratinolytic Bacillus...N. Tandan. 1950. A new wool degrading fungus-Ctenomyces...in thermophilic poultry waste digesters and enrichment of a feather-degrading...

C. M. Williams; C. S. Richter; J. M. MacKenzie Jr.; Jason C. H. Shih

1990-06-01T23:59:59.000Z

70

Use of thermophilic bacteria for bioremediation of petroleum contaminants  

SciTech Connect (OSTI)

Several strains of thermophilic bacteria were isolated from the environment of the United Arab Emirates. These bacteria show extraordinary resistance to heat and have their maximum growth rate around 60--80 C. This article investigates the potential of using these facultative bacteria for both in situ and ex situ bioremediation of petroleum contaminants. In a series of batch experiments, bacterial growth was observed using a computer image analyzer following a recently developed technique. These experiments showed clearly that the growth rate is enhanced in the presence of crude oil. This is coupled with a rapid degradation of the crude oil. These bacteria were found to be ideal for breaking down long-chain organic molecules at a temperature of 40 C, which is the typical ambient temperature of the Persian Gulf region. The same strains of bacteria are also capable of surviving in the presence of the saline environment that can prevail in both sea water and reservoir connate water. This observation prompted further investigation into the applicability of the bacteria in microbial enhanced oil recovery. In the United Arab Emirates, the reservoirs are typically at a temperature of around 85 C. Finally, the performance of the bacteria is tested in a newly developed bioreactor that uses continuous aeration through a transverse slotted pipe. This reactor also uses mixing without damaging the filamentous bacteria. In this process, the mechanisms of bioremediation are identified.

Al-Maghrabi, I.M.A.; Bin Aqil, A.O.; Chaalal, O. [United Arab Emirates Univ., Al-Ain (United Arab Emirates); Islam, M.R. [Univ. of Regina, Saskatchewan (Canada). Faculty of Engineering

1999-01-01T23:59:59.000Z

71

Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

design design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles Olson and Lynd Olson and Lynd Journal of Biological Engineering 2012, 6:5 http://www.jbioleng.org/content/6/1/5 R E S E A R C H Open Access Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles Daniel G Olson 1,3 and Lee R Lynd 1,2,3* Abstract Background: Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional mutagenesis techniques yield Ts mutants at a low frequency, and therefore requires the development of high-throughput screening protocols, which are also not available for this organism. Recently there has been progress in the development

72

One-step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

step step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis Xiao-Zhou Zhang a , Noppadon Sathitsuksanoh a,b , Zhiguang Zhu a , Y.-H. Percival Zhang a,b,c,n a Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA b Institute for Critical Technology and Applied Science, Virginia Tech, Blacksburg, VA 24061, USA c BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA a r t i c l e i n f o Article history: Received 28 December 2010 Received in revised form 9 April 2011 Accepted 25 April 2011 Keywords: Bacillus subtilis Cellulase engineering Consolidated bioprocessing Endoglucanase Lactate Metabolic engineering Directed evolution a b s t r a c t Although intensive efforts have been made to create recombinant cellulolytic microorganisms,

73

Quantitative Influences of Butyrate or Propionate on Thermophilic Production of Methane from Biomass  

Science Journals Connector (OSTI)

...Propionate on Thermophilic Production of Methane from Biomass...Microbiology and Cell Science, University...Present address: Solar Energy Research Institute...new stable external organic acid pool sizes and new stable gas production rates were observed...Microbiology and Cell Science, University...

J. Michael Henson; F. M. Bordeaux; Christopher J. Rivard; P. H. Smith

1986-02-01T23:59:59.000Z

74

Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass  

Science Journals Connector (OSTI)

...thermophilic conditions using green waste compost as the inoculum...were purchased from a municipal green waste compositing facility...52N, 12117.35W). The green waste consisted of yard trimmings...sole supplemented carbon and energy source in 50 ml of M9 medium...

Stephanie A. Eichorst; Chijioke Joshua; Noppadon Sathitsuksanoh; Seema Singh; Blake A. Simmons; Steven W. Singer

2014-09-26T23:59:59.000Z

75

Sequential two-step conversion of 4-oxoisophorone to 4-hydroxy-2,2,6-trimethylcyclohexanone by thermophilic bacteria  

Science Journals Connector (OSTI)

The sequential two-step conversion of 4-oxoisophorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) via diyhdrooxoisophorone (DOIP) was achieved using two kinds of thermophiles, Thermomonospora curvat...

Kunio Nishii; Koji Sode; Isao Karube

1990-06-01T23:59:59.000Z

76

Differences in the catalytic mechanisms of mesophilic and thermophilic indole-3-glycerol phosphate synthase enzymes at their adaptive temperatures  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Catalytic mechanisms of thermophilic-mesophilic enzymes may differ. Black-Right-Pointing-Pointer Product release is rate-determining for thermophilic IGPS at low temperatures. Black-Right-Pointing-Pointer But at higher temperatures, proton transfer from the general acid is rate-limiting. Black-Right-Pointing-Pointer Rate-determining step is different still for mesophilic IGPS. Black-Right-Pointing-Pointer Both chemical and physical steps of catalysis are important for temperature adaptation. -- Abstract: Thermophilic enzymes tend to be less catalytically-active at lower temperatures relative to their mesophilic counterparts, despite having very similar crystal structures. An often cited hypothesis for this general observation is that thermostable enzymes have evolved a more rigid tertiary structure in order to cope with their more extreme, natural environment, but they are also less flexible at lower temperatures, leading to their lower catalytic activity under mesophilic conditions. An alternative hypothesis, however, is that complementary thermophilic-mesophilic enzyme pairs simply operate through different evolutionary-optimized catalytic mechanisms. In this communication, we present evidence that while the steps of the catalytic mechanisms for mesophilic and thermophilic indole-3-glycerol phosphate synthase (IGPS) enzymes are fundamentally similar, the identity of the rate-determining step changes as a function of temperature. Our findings indicate that while product release is rate-determining at 25 Degree-Sign C for thermophilic IGPS, near its adaptive temperature (75 Degree-Sign C), a proton transfer event, involving a general acid, becomes rate-determining. The rate-determining steps for thermophilic and mesophilic IGPS enzymes are also different at their respective, adaptive temperatures with the mesophilic IGPS-catalyzed reaction being rate-limited before irreversible CO{sub 2} release, and the thermophilic IGPS-catalyzed reaction being rate limited afterwards.

Zaccardi, Margot J.; Mannweiler, Olga [Department of Chemistry, The Pennsylvania State University, University Park, PA 16802 (United States)] [Department of Chemistry, The Pennsylvania State University, University Park, PA 16802 (United States); Boehr, David D., E-mail: ddb12@psu.edu [Department of Chemistry, The Pennsylvania State University, University Park, PA 16802 (United States)

2012-02-10T23:59:59.000Z

77

Genome sequence of the moderately thermophilic, amino-acid-degrading and sulfur-reducing bacterium Thermovirga lienii type strain (Cas60314T)  

SciTech Connect (OSTI)

Thermovirga lienii Dahle and Birkeland 2006 is a member to the genomically so far uncharacterized genus Thermovirga in the phylum 'Synergistetes'. Members of the only recently (2007) proposed phylum 'Synergistetes' are of interest because of their isolated phylogenetic position and their diverse habitats, e.g. from man to oil well. The genome of T. lienii Cas60314T is only the 5th genome sequence (3rd completed) from this phylum to be published. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 1,999,646 bp long genome (including one plasmid) with its 1,914 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Saunders, Elizabeth H [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Chang, Yun-Juan [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2012-01-01T23:59:59.000Z

78

Genome sequence of the moderately thermophilic, amino-acid-degrading and sulfur-reducing bacterium Thermovirga lienii type strain (Cas60314T)  

E-Print Network [OSTI]

Lynne A. Goodwin 2,3 , Sam Pitluck 2 , Konstantinos LioliosM, Tice H, Cheng JF, Pitluck S, Liolios K, et al. PermanentGenomic Sci 2010; 3:85-92. Pitluck S, Yasawong M, Held B,

Goker, Markus

2014-01-01T23:59:59.000Z

79

The HPr Proteins from the Thermophile Bacillus stearothermophilus Can Form Domain-swapped Dimers  

SciTech Connect (OSTI)

The study of proteins from extremophilic organisms continues to generate interest in the field of protein folding because paradigms explaining the enhanced stability of these proteins still elude us and such studies have the potential to further our knowledge of the forces stabilizing proteins. We have undertaken such a study with our model protein HPr from a mesophile, Bacillus subtilis, and a thermophile, Bacillus stearothermophilus. We report here the high-resolution structures of the wild-type HPr protein from the thermophile and a variant, F29W. The variant proved to crystallize in two forms: a monomeric form with a structure very similar to the wild-type protein as well as a domain-swapped dimer. Interestingly, the structure of the domain-swapped dimer for HPr is very different from that observed for a homologous protein, Crh, from B. subtilis. The existence of a domain-swapped dimer has implications for amyloid formation and is consistent with recent results showing that the HPr proteins can form amyloid fibrils. We also characterized the conformational stability of the thermophilic HPr proteins using thermal and solvent denaturation methods and have used the high-resolution structures in an attempt to explain the differences in stability between the different HPr proteins. Finally, we present a detailed analysis of the solution properties of the HPr proteins using a variety of biochemical and biophysical methods.

Sridharan, Sudharsan; Razvi, Abbas; Scholtz, J. Martin; Sacchettini, James C. (TAM)

2010-07-20T23:59:59.000Z

80

Thermophilic Anaerobic Digestion to Increase the Net Energy Balance of Corn Grain Ethanol  

Science Journals Connector (OSTI)

Thermophilic Anaerobic Digestion to Increase the Net Energy Balance of Corn Grain Ethanol ... However, the calculation did not include the energetic costs to physically replace the evaporator with the integrated digester system (this will be a relatively small fraction of the energy input because the percentage of energy input per unit of ethanol energy output for construction of the entire conventional dry mill is 0.2% (4)); the improved quality in animal feed (DDG vs DDGS); nor the available waste heat from circumventing thin stillage evaporation. ...

Matthew T. Agler; Marcelo L. Garcia; Eric S. Lee; Martha Schlicher; Largus T. Angenent

2008-08-05T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus  

Science Journals Connector (OSTI)

The genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus shows numerous distinctive features that may shine light on the origin and early evolution of photosynthesis.

Kuo-Hsiang Tang; Kerrie Barry; Olga Chertkov; Eileen Dalin; Cliff S Han; Loren J Hauser; Barbara M Honchak; Lauren E Karbach; Miriam L Land; Alla Lapidus; Frank W Larimer; Natalia Mikhailova; Samuel Pitluck; Beverly K Pierson; Robert E Blankenship

2011-06-29T23:59:59.000Z

82

E-Print Network 3.0 - ammonia oxidizing bacterium Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

0099-22409804.00 0 Summary: acetate oxidation with electron transfer to an anaerobic partner bacterium in the absence of ferric iron... , a syntrophic coculture of a...

83

Ultrastable phosphoglucose isomerase through immobilization of cellulosebinding moduletagged thermophilic enzyme on lowcost highcapacity cellulosic adsorbent  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Ultra-stable phosphoglucose isomerase through immobilization of cellulose- Ultra-stable phosphoglucose isomerase through immobilization of cellulose- binding module-tagged thermophilic enzyme on low-cost high-capacity cellulosic adsorbent Suwan Myung 1,2 , Xiao-Zhou Zhang 1 , Y.-H. Percival Zhang 1,2,3* Running title: One-step protein purification and immobilization 1 Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA 2 Institute for Critical Technology and Applied Science (ICTAS), Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA 3 DOE BioEnergy Science Center (BESC), Oak Ridge, TN 37831, USA *Corresponding author. Tel: 540-231-7414; Fax: 540-231-7414; Email: ypzhang@vt.edu Biocatalysts and Bioreactor Design

84

Thermophilic Thermotoga maritima ribose-5-phosphate isomerase RpiB: Optimized heat treatment purification and basic characterization  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Thermophilic Thermophilic Thermotoga maritima ribose-5-phosphate isomerase RpiB: Optimized heat treatment purification and basic characterization Fangfang Sun a , Xiao-Zhou Zhang a,b , Suwan Myung a,c , Y.-H. Percival Zhang a,b,c,d,⇑ a Biological Systems Engineering Department, 304 Seitz Hall, Virginia Tech, Blacksburg, VA 24061, USA b Gate Fuels Inc., 3107 Alice Drive, Blacksburg, VA 24060, USA c Institute for Critical Technology and Applied Science (ICTAS), Virginia Tech, Blacksburg, VA 24061, USA d DOE BioEnergy Science Center (BESC), Oak Ridge, TN 37831, USA a r t i c l e i n f o Article history: Received 27 December 2011 and in revised form 26 January 2012 Available online 8 February 2012 Keywords: Biofuel Cascade enzyme factories Heat treatment purification Pentose phosphate isomerase Ribose-5-phosphate isomerase Thermoenzyme a b s t r a c t The open reading frame TM1080 from Thermotoga

85

Bacterium Helps in the Formation of Gold | Advanced Photon Source  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Creating a Precise Atomic-Scale Map of Quantum Dots Creating a Precise Atomic-Scale Map of Quantum Dots Getting to the Roots of Lethal Hairs Stressing Out the Twins in Magnesium Size-Related Behavior of Anatase Nanocrystals under Extreme Pressure Boron-Based Compounds Trick a Biomedical Protein Science Highlights Archives: 2013 | 2012 | 2011 | 2010 2009 | 2008 | 2007 | 2006 2005 | 2004 | 2003 | 2002 2001 | 2000 | 1998 | Subscribe to APS Science Highlights rss feed Bacterium Helps in the Formation of Gold OCTOBER 13, 2009 Bookmark and Share Top: An ultra-thin section of C. metallidurans containing a gold nanoparticle. Bottom: This image shows maps of pure gold with other elements. By determining what elements there are, scientists can see where the gold is located in relation to the cells. These maps are quantitative

86

Why sequence radiation-resistant bacterium Deinococcus grandis?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

radiation-resistant radiation-resistant bacterium Deinococcus grandis? A fifth of the United States' electricity is generated from nuclear power, which can also be used for medical procedures and other applications. The radioactive waste generated by nuclear reactors, hospitals and universities need to be disposed of in specially selected sites. Deinococcus bacteria have the capacity to add electrons to a variety of metals, including uranium, chromium, mercury, technetium, iron and manganese. Due to this unique characteristic, this group of extremely radiation-resistant bacteria has been considered as a prospective candidate to help clean up radioactive waste sites. However, many Deinococcus bacteria require oxygen, which is a problem considering most waste environments are anaerobic.

87

The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics  

Science Journals Connector (OSTI)

...restrictus: lessons from tiered functional genomics Aamani Rupakula 1 Thomas Kruse 2 Sjef...describes a multi-level functional genomics approach on D. restrictus across three...OHR bacterium using a tiered functional genomics approach. 2. Material and methods...

2013-01-01T23:59:59.000Z

88

Thermophilic α-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization and enhanced thermostability  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Molecular Molecular Catalysis B: Enzymatic 65 (2010) 110-116 Contents lists available at ScienceDirect Journal of Molecular Catalysis B: Enzymatic j o u r n a l h o m e p a g e : w w w . e l s e v i e r . c o m / l o c a t e / m o l c a t b Thermophilic ␣-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization and enhanced thermostability Xinhao Ye a , Joe Rollin a , Yi-Heng Percival Zhang a,b,c,∗ a Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA b Institute for Critical Technology and Applied Science (ICTAS), Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA c DOE Bioenergy Science Center, Oak Ridge, TN 37831, USA a r t i c l e i n f o Article history: Available online 25 January 2010 Keywords: ␣-Glucan phosphorylase Biocatalysis Building

89

A single domain thermophilic xylanase can bind insoluble xylan: evidence for surface aromatic clusters  

Science Journals Connector (OSTI)

A clone expressing xylanase activity in Escherichia coli has been selected from a genomic plasmid library of the thermophilic Bacillus strain D3. Subcloning from the 9-kb insert located the xylanase activity to a 2.7-kb HindII/BamHI fragment. The DNA sequence of this clone revealed an ORF of 367 codons encoding a single domain type-F or family 10 enzyme, which was designated as XynA. Purification of the enzyme following over-expression in E. coli produced an enzyme of 42 kDa with a temperature optimum of 75°C which can efficiently bind and hydrolyse insoluble xylan. The pH optimum of the enzyme is 6.5, but it is active over a broad pH range. A homology model of the xylanase has been constructed which reveals a series of surface aromatic residues which form hydrophobic clusters. This unusual structural feature is strikingly similar to the situation observed in the structure determined for the type-G xylanase from the Bacillus D3 strain and may constitute a common evolutionary mechanism imposed on different structural frameworks by which these xylanases may bind potential substrates and exhibit thermostability.

Ian Connerton; Nicola Cummings; Gillian W. Harris; Philippe Debeire; Christelle Breton

1999-01-01T23:59:59.000Z

90

Effect of bacterium Oceanospirillum on the corrosion potential and oxygen reduction of AISI 4340 steel  

E-Print Network [OSTI]

EFFECT OF BACTERIUM OCEANOSPIRILLUM ON THE CORROSION POTENTIAL AND OXYGEN REDUCI1ON OF AISI 4340 STEEL A Thesis by SNEZANA N. POPOVA Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE December 1992 Major Subject: Chemical Engineering EFFECT OF BACTERIUM OCEANOSPIRILLUM ON THE CORROSION POTENTIAL AND OXYGEN REDUCTION OF AISI 4340 STEEL A Thesis by SNEZANA N. POPOVA Appmved as to style...

Popova, Snezana N.

2012-06-07T23:59:59.000Z

91

Obligate autotrophy in the ammonia oxidizing bacterium Nitrosomonas europaea.  

SciTech Connect (OSTI)

Closing report for project DOE-FG02-03ER15436. The project studied obligate autotrophy in the ammonia oxidizing bacterium Nitrosomonas europaea. Nitrosomonas europaea can obtain all of its energy and reductant for growth from the oxidation of ammonia to nitrite and is, therefore, classified as a chemolithotroph. This bacterium is also an autotroph, which can derive all cellular carbon from carbon dioxide. N. europaea seems incapable of growth with other carbon or energy sources. This restricted capability is surprising given that ammonia is a poor energy source. The main goal of the project was to examine the basis of autotrophy in N. europaea or, thought of another way, to determine the barriers to heterotrophy. The approach was enabled by the N. europaea genome sequence, stimulating new ways of thinking about this physiological paradox—an insistence on a single, albeit poor, energy source. Objective 1 was to examine the expression and regulation of the genes coding for alpha-ketoglutarate dehydrogenase, determine if the enzyme’s activity is present, and determine whether alteration of the expression levels influences autotrophic growth. Although Nitrosomonas europaea lacks measurable alpha-ketoglutarate dehydrogenase activity, the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the E1 subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase. The results were published in Hommes N.G., Kurth E. G., Sayavedra-Soto L.A., and Arp D.J. (2006) Disruption of sucA, which encodes a subunit of alpha-ketoglutarate dehydrogenase, affects the survival of Nitrosomonas europaea in stationary phase. Journal of Bacteriology 188:343-347. Objective 2 was to determine the basis of fructose stimulation of growth on ammonia, examine fructose metabolism, and determine the impact of other compounds on growth on ammonia. Previous studies showed that N. europaea can utilize limited amounts of certain organic compounds, including amino acids, pyruvate, and acetate, although no organic compound has been reported to support the growth of N. europaea. The genomic sequence of N. europaea revealed a potential permease for fructose. N. europaea utilized fructose and other compounds as carbon sources to support growth. Cultures were incubated in the presence of fructose or other organic compounds in sealed bottles purged of CO(2). In these cultures, addition of either fructose or pyruvate as the sole carbon source resulted in a two- to threefold increase in optical density and protein content in 3 to 4 days. Studies with [(14)C]fructose showed that >90% of the carbon incorporated by the cells during growth was derived from fructose. Cultures containing mannose, glucose, glycerol, mannitol, citrate, or acetate showed little or no growth. N. europaea was not able to grow with fructose as an energy source, although the presence of fructose did provide an energy benefit to the cells. These results show that N. europaea can be grown in carbon dioxide free medium by using fructose and pyruvate as carbon sources and may now be considered a facultative chemolithoorganotroph. The results were published in Hommes N.G., Sayavedra-Soto L.A. and Arp. D.J. (2003). Chemolithotrophic growth of Nitrosomonas europaea on fructose. Journal of Bacteriology. 185:6809-2773. Objective 3 attempted to grow N. europaea heterotrophically through pathways predicted by the genome. Experiments with mutant strains and complementation studies were performed to test whether N. europaea can utilize other carbon sources. N. europaea was not able to grow heterotrophically in the conditions tested in this objective.

Daniel James Arp; Luis Alberto Sayavedra-Soto

2006-01-01T23:59:59.000Z

92

Complete genome sequence of the moderate thermophile Anaerobaculum mobile type strain (NGAT)  

SciTech Connect (OSTI)

Anaerobaculum mobile Menes and Muxi 2002 is one of three described species of the genus Anaerobaculum, family Synergistaceae, phylum Synergistetes. This anaerobic and motile bac- terium ferments a range of carbohydrates and mono- and dicarboxylic acids with acetate, hy- drogen and CO2 as end products. A. mobile NGAT is the first member of the genus Anaerobaculum and the sixth member of the phylum Synergistetes with a completely se- quenced genome. Here we describe the features of this bacterium, together with the com- plete genome sequence, and annotation. The 2,160,700 bp long single replicon genome with its 2,053 protein-coding and 56 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Stackebrandt, Erko [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Held, Brittany [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute

2013-01-01T23:59:59.000Z

93

Are you protected against Pertussis? Pertussis, or whooping cough, is a highly contagious respiratory infection caused by the bacterium  

E-Print Network [OSTI]

Are you protected against Pertussis? Pertussis, or whooping cough, is a highly contagious respiratory infection caused by the bacterium Bordetella pertussis. It causes severe coughing spells, vomiting

94

A plant growth-promoting bacterium that decreases nickel toxicity in seedlings  

SciTech Connect (OSTI)

A plant growth-promoting bacterium, Kluyvera ascorbata SUD165, that contained high levels of heavy metals was isolated from soil collected near Sudbury, Ontario, Canada. The bacterium was resistant to the toxic effects of Ni{sup 2+}, Pb{sup 2+}, Zn{sup 2+}, and CrO{sub 4}{sup {minus}}, produced a siderophore(s), and displayed 1-aminocyclopropane-1-carboxylic acid deaminase activity. Canola seeds inoculated with this bacterium and then grown under gnotobiotic conditions in the presence of high concentrations of nickel chloride were partially protected against nickel toxicity. In addition, protection by the bacterium against nickel toxicity was evident in pot experiments with canola and tomato seeds. The presence of K. ascorbata SUD165 had no measurable influence on the amount of nickel accumulated per milligram (dry weight) of either roots or shoots of canola plants. Therefore, the bacterial plant growth-promoting effect in the presence of nickel was probably not attributable to the reduction of nickel uptake by seedlings. Rather, it may reflect the ability of the bacterium to lower the level of stress ethylene induced by the nickel.

Burd, G.I.; Dixon, D.G.; Glick, B.R. [Univ. of Waterloo, Ontario (Canada). Dept. of Biology

1998-10-01T23:59:59.000Z

95

1H, 13C, and 15N backbone and side chain resonance assignments of thermophilic Geobacillus kaustophilus cyclophilin-A  

SciTech Connect (OSTI)

Cyclophilins catalyze the reversible peptidyl-prolyl isomerization of their substrates and are present across all kingdoms of life from humans to bacteria. Although numerous biological roles have now been discovered for cyclophilins, their function was initially ascribed to their chaperone-like activity in protein folding where they catalyze the often rate-limiting step of proline isomerization. This chaperone-like activity may be especially important under extreme conditions where cyclophilins are often over expressed, such as in tumors for human cyclophilins {Lee, 2010 #1167}, but also in organisms that thrive under extreme conditions, such as theromophilic bacteria. Moreover, the reversible nature of the peptidyl-prolyl isomerization reaction catalyzed by cyclophilins has allowed these enzymes to serve as model systems for probing the role of conformational changes during catalytic turnover {Eisenmesser, 2002 #20;Eisenmesser, 2005 #203}. Thus, we present here the resonance assignments of a thermophilic cyclophilin from Geobacillus kaustophilus derived from deep-sea sediment {Takami, 2004 #1384}. This thermophilic cyclophilin may now be studied at a variety of temperatures to provide insight into the comparative structure, dynamics, and catalytic mechanism of cyclophilins.

Holliday, Michael; Zhang, Fengli; Isern, Nancy G.; Armstrong, Geoffrey S.; Eisenmesser, Elan Z.

2014-04-01T23:59:59.000Z

96

In Situ Expression of Acidic and Thermophilic Carbohydrate Active Enzymes by Filamentous Fungi (JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)  

ScienceCinema (OSTI)

Annika Mosier, graduate student from Stanford University presents a talk titled "In Situ Expression of Acidic and Thermophilic Carbohydrate Active Enzymes by Filamentous Fungi" at the JGI User 7th Annual Genomics of Energy & Environment Meeting on March 22, 2012 in Walnut Creek, Calif

Mosier, Annika [Stanford University

2013-01-22T23:59:59.000Z

97

Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1  

SciTech Connect (OSTI)

Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 C and pH 5.0 and fer- ments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this spo- rogenic lactic acid bacterium to grow at 50-55 C and pH 5.0 makes this organism an attrac- tive microbial biocatalyst for production of optically pure lactic acid at industrial scale not only from glucose derived from cellulose but also from xylose, a major constituent of hemi- cellulose. This bacterium is also considered as a potential probiotic. Complete genome se- quence of a representative strain, B. coagulans strain 36D1, is presented and discussed.

Rhee, Mun Su [University of Florida, Gainesville; Moritz, Brelan E. [University of Florida, Gainesville; Xie, Gary [Los Alamos National Laboratory (LANL); Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Chertkov, Olga [Los Alamos National Laboratory (LANL); Brettin, Thomas S [ORNL; Han, Cliff [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Patel, Milind [University of Florida, Gainesville; Ou, Mark [University of Florida, Gainesville; Harbrucker, Roberta [University of Florida, Gainesville; Ingram, Lonnie O. [University of Florida; Shanmugam, Keelnathan T. [University of Florida

2011-01-01T23:59:59.000Z

98

Cellulolytic and non-cellulolytic bacteria in rat gastrointestinal tracts.  

Science Journals Connector (OSTI)

...that the addi- tion of bran or corn bean to a diet increased activities...The PMC was prepared by pebble milling 20 g of Whatman no. 1 filter...stick, approximately 1 g (wet weight) of material was transferred...bacteria per gram of contents (wet weight) was approximately five...

J M Macy; J R Farrand; L Montgomery

1982-12-01T23:59:59.000Z

99

Microsoft Word - _FINAL_JB246-09_AT_032609.doc  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

J. Bacteriol. J. Bacteriol. (Revised 03/26/09: JB256-09) Genome Announcement Genome Sequence of the Anaerobic, Thermophilic and Cellulolytic Bacterium Anaerocellum thermophilum DSM 6725 Irina A. Kataeva 1,4 , Sung-Jae Yang 1,4 , Phuongan Dam 1,3,4 , Farris L Poole II 1 , Yanbin Yin 1,3,4 , Fengfeng Zhou 1,3,4 , Wen-chi Chou 1,3,4 , Ying Xu 1,3,4 , Lynne Goodwin 6 , David R. Sims 6 , John C. Detter 6 , Loren J. Hauser 5 , Janet Westpheling 2,4 and Michael W. W. Adams 1,4 * 1 Department of Biochemistry and Molecular Biology, 2 Department of Genetics and 3 Institute of Bioinformatics, University of Georgia, GA 30602; 4 BioEnergy Science Center and 5 Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831;

100

The oxidation of ethylene glycol by Bacterium T-52: mutagenesis studies  

E-Print Network [OSTI]

of the isolated inter. sedis te. DIS USSIOH 19 19 40 40 43 66 SU: il iARY ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ 0 ~ ~ ~ \\ 72 LIT RATUIlE' CITZ3 VITA ~ ~ ~ 73 77 LIST OF TABLES Table 1 ~ Generation times of wild type and mutants grotring on glycerol as carbon... curve relating dry weight of this bacterium to O. D. was constructed for this purpose. Generation time was calculated graphically from growth curves and i'rom the relationship: gt= log10 2(t1-tO)/ log100. D. 1-log100. D. O. Growth oi' Bacterium T-52...

Smith, Frank Judson

1976-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

THE ANAEROBIC MESOPHILIC CELLULOLYTIC BACTERIA  

Science Journals Connector (OSTI)

...forms. The fact that the old rods become spherical suggest that no rigid cell wall is present...tube 3 there developed non-filamentous spherical colonies with an irregular surface...consted of gram negative rods with terminal spherical spores whereas the spherical colonies...

R. E. Hungate

1950-03-01T23:59:59.000Z

102

The Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury  

E-Print Network [OSTI]

Bacterial Mercury Methylation Contact: Cynthia Gilmour (gilmourc@si.edu, 443-482-2498) DOE/Office of Science/Biological & Environmental Research ·The ORNL Mercury Science Focus Area is developing the Hg-methylating bacterium as a model for understanding bacterial mercury methylation. Appl. Environ. Microbiol. 77:3938-3951 (doi:10

103

The biodegradation of olive oil and the treatment of lipid-rich wool scouring wastewater under aerobic thermophilic conditions  

Science Journals Connector (OSTI)

The biodegradation of olive oil and the treatment of the lipid-rich wool scouring wastewater were studied under aerobic thermophilic (65°C) conditions using the newly isolated strain Bacillus thermoleovorans IHI-91. In a continuously operated laboratory-scale stirred-tank reactor olive oil was degraded to more than 90% at a residence time of 2 h with a maximum volumetric degradation rate of 900 mg l?1 h?1. A relatively high maximum biomass yield of 1.05 g dry cell weight per g olive oil consumed was measured and a maintenance coefficient of 0.04 g olive oil (g DCW)?1 h?1 was calculated from steady-state data. A severe growth inhibition was observed when the feed olive oil concentration was increased to more than 4 g l?1. Lipid removal from the highly loaded wool scouring wastewater (COD of 77000 mg l?1) was 20–30% at a residence time of 10–20 h while the COD removal was 15–20%. GC-MS analysis revealed that longchain fatty acids up to C18 were efficiently degraded while degradation of the predominant sterol-fraction of the wool grease was not detected. The high concentrations of volatile fatty acids (60 mM acetic acid, 13 mM propionic acid) present in the wastewater were completely removed even at residence times of 2 h. Compared with data from mesophilic processes the lipid degradation rates obtained under thermophilic conditions are extremely high.

P Becker; D Köster; M.N Popov; S Markossian; G Antranikian; H Märkl

1999-01-01T23:59:59.000Z

104

Scientists decipher genome of bacterium that remediates uranium contamination, generates electricity Public release date: 11-Dec-2003  

E-Print Network [OSTI]

a microbe's capability to generate electricity and to help clean up radioactive contamination, scientistsScientists decipher genome of bacterium that remediates uranium contamination, generates that remediates uranium contamination, generates electricity Analysis of Geobacter sulfurreducens genes reveals

Lovley, Derek

105

A BACTERIUM CAPABLE OF USING PHYTOL AS ITS SOLE CARBON SOURCE, ISOLATED FROM ALGAL SEDIMENT OF MUD LAKE, FLORIDA  

Science Journals Connector (OSTI)

...isoprenoid hydrocarbons found in oil shale of the Green River Formation (Eocene...through geologic time, became oil shale. A bacterium capable of using phytol...isoprenoid hydrocarbons found in oil shale of the Green River Formation (Eocene...

K. B. Hoag; W. H. Bradley; A. J. Tousimis; D. L. Price

1969-01-01T23:59:59.000Z

106

Thermophilic anaerobic digestion of thermal pretreated sludge: Role of microbial community structure and correlation with process performances  

Science Journals Connector (OSTI)

Abstract Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances. In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate. 16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process. The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions.

M.C. Gagliano; C.M. Braguglia; A. Gianico; G. Mininni; K. Nakamura; S. Rossetti

2015-01-01T23:59:59.000Z

107

Thermophilic hydrogen production from starch wastewater using two-phase sequencing batch fermentation coupled with UASB methanogenic effluent recycling  

Science Journals Connector (OSTI)

Abstract The aim of this study was to evaluate the performance of thermophilic hydrogenesis coupled with mesophilic methanogenesis in which the effluent was recycled to the hydrogen reactor for starch wastewater treatment. With this system, the hydrogen production rate and yield were 3.45 ± 0.25 L H2/(L·d) and 5.79 ± 0.41 mmol H2/g \\{CODadded\\} respectively, and thus higher than the values of the control group without methanogenic effluent recycling. In addition, relatively higher contents of acetate and butyrate were obtained in the hydrogen reactor with recirculation. The methane reactors were operated with the effluent from the hydrogen reactor, and methane yield was stabilized at 0.21–0.23 L/g \\{CODremoval\\} in both. Analysis of the microbial communities further showed that methanogenic effluent recirculation enriched microbial communities in the hydrogen reactor. Two species of bacteria effective in hydrogenesis, Thermoanaerobacterium thermosaccharolyticum and Clostridium thermosaccharolyticum, dominated during hydrogen production, whereas archaea belonging to Euryarchaeota were detected and cultured in the methane reactor. The recycled effluent supplied alkaline substrates for the hydrogen producing bacteria. Alkali balance calculations showed that the amount of added alkali was reduced by 88%. This amount, required for hydrogen production from starch wastewater, was contributed by alkali in the methanogenic effluent, (2225 ± 140 mg CaCO3/L), resulting in lower operational costs.

Li Xie; Nanshi Dong; Lei Wang; Qi Zhou

2014-01-01T23:59:59.000Z

108

Response to Comments on "A Bacterium That Can Grow Using Arsenic Instead of Phosphorus"  

SciTech Connect (OSTI)

Concerns have been raised about our recent study describing a bacterium that can grow using arsenic (As) instead of phosphorus (P). Our data suggested that As could act as a substitute for P in major biomolecules in this organism. Although the issues raised are of investigative interest, we contend that they do not invalidate our conclusions. We argue that while no single line of evidence we presented was sufficient to support our interpretation of the data, taken as an entire dataset we find no plausible alternative to our conclusions. Here we reply to the critiques and provide additional arguments supporting the assessment of the data we reported.

Wolfe-Simon, F; Blum, J S; Kulp, T R; Gordon, G W; Hoeft, S E; Pett-Ridge, J; Stolz, J F; Webb, S M; Weber, P K; Davies, P W; Anbar, A D; Oremland, R S

2011-03-07T23:59:59.000Z

109

A bacterium that can grow by using arsenic instead of phosphorus  

SciTech Connect (OSTI)

Life is mostly composed of the elements carbon, hydrogen, nitrogen, oxygen, sulfur and phosphorus. Although these six elements make up nucleic acids, proteins and lipids and thus the bulk of living matter, it is theoretically possible that some other elements in the periodic table could serve the same functions. Here we describe a bacterium, strain GFAJ-1 of the Halomonadaceae, isolated from Mono Lake, CA, which substitutes arsenic for phosphorus to sustain its growth. Our data show evidence for arsenate in macromolecules that normally contain phosphate, most notably nucleic acids and proteins. Exchange of one of the major bio-elements may have profound evolutionary and geochemical significance.

Wolfe-Simon, F; Blum, J S; Kulp, T R; Gordon, G W; Hoeft, S E; Pett-Ridge, J; Stolz, J F; Webb, S M; Weber, P K; Davies, P W; Anbar, A D; Oremland, R S

2010-11-01T23:59:59.000Z

110

Draft Genome Sequence for Microbacterium laevaniformans Strain OR221, a Bacterium Tolerant to Metals, Nitrate, and Low pH  

SciTech Connect (OSTI)

Microbacterium laevaniformans strain OR221 was isolated from subsurface sediments obtained from the Field Research Center (FRC) in Oak Ridge, TN. It was characterized as a bacterium tolerant to heavy metals such as uranium, nickel, cobalt, cadmium, as well as nitrate and low pH. We present its draft genome sequence.

Brown, Steven D [ORNL; Palumbo, Anthony Vito [ORNL; Panikov, Nikolai [ORNL; Ariyawansa, Thilini [Northeastern University; Klingeman, Dawn Marie [ORNL; Johnson, Courtney M [ORNL; Land, Miriam L [ORNL; Utturkar, Sagar M [ORNL; Epstein, Slava [Northeastern University

2012-01-01T23:59:59.000Z

111

From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov.  

Science Journals Connector (OSTI)

...throughout the salinity gradient of a coastal solar saltern. Environ. Microbiol. 4 :349-360...fingerprinting methods in a multipond solar saltern. Environ. Microbiol. 4 :338-348...sulfur-reducing bacterium isolated from a sulfide chimney in Suiyo Seamount. Int. J. Syst. Evol...

María José León; Ana B. Fernández; Rohit Ghai; Cristina Sánchez-Porro; Francisco Rodriguez-Valera; Antonio Ventosa

2014-04-18T23:59:59.000Z

112

Volume exclusion and elasticity driven directional transport: an alternative model for bacterium motility  

E-Print Network [OSTI]

On the basis of a model we capture the role of strong attractive interaction in suppressing the rotational degrees of freedom of the system and volume exclusion in keeping microscopic symmetry-breaking intact to result in super-diffusive transport of small systems in a thermal atmosphere over a large time scale. Our results, characterize such systems on the basis of having a super-diffusive intermediate regime in between a very small and large time scales of diffusive regimes. Although, the Brownian ratchet model fails to account for the origin of motility in actin polymerization propelled directional motion of bacterium like Listeria Monocytogene (LM) and similar bio-mimetic systems due to the presence of strong attractive forces, our model can account for the origin of directional transport in such systems on the basis of the same interactions.

A. Bhattacharyay

2009-05-14T23:59:59.000Z

113

Volume exclusion and elasticity driven directional transport: an alternative model for bacterium motility  

E-Print Network [OSTI]

On the basis of a model we capture the role of strong attractive interaction in suppressing the rotational degrees of freedom of the system and volume exclusion in keeping microscopic symmetry-breaking intact to result in super-diffusive transport of small systems in a thermal atmosphere over a large time scale. Our results, characterize such systems on the basis of having a super-diffusive intermediate regime in between a very small and large time scales of diffusive regimes. Although, the Brownian ratchet model fails to account for the origin of motility in actin polymerization propelled directional motion of bacterium like Listeria Monocytogene (LM) and similar bio-mimetic systems due to the presence of strong attractive forces, our model can account for the origin of directional transport in such systems on the basis of the same interactions.

Bhattacharyay, A

2009-01-01T23:59:59.000Z

114

Enzyme inactivation by ethanol and development of a kinetic model for thermophilic simultaneous saccharification and fermentation at 50C with Thermoanaerobacterium saccharolyticum ALK2  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Enzyme Enzyme Inactivation by Ethanol and Development of a Kinetic Model for Thermophilic Simultaneous Saccharification and Fermentation at 50 -C with Thermoanaerobacterium saccharolyticum ALK2 Kara K. Podkaminer, 1 Xiongjun Shao, 1 David A. Hogsett, 2 Lee R. Lynd 1 1 Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755; telephone: 603-646-2231; fax: 603-646-2277; e-mail: lee.lynd@dartmouth.edu 2 Mascoma Corporation, Lebanon, New Hampshire Received 6 August 2010; revision received 16 November 2010; accepted 14 December 2010 Published online 29 December 2010 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/bit.23050 ABSTRACT: Studies were undertaken to understand phe- nomena operative during simultaneous saccharification and fermentation (SSF) of a model cellulosic substrate (Avicel) at 508C with enzymatic hydrolysis mediated

115

Effects of selected thermophilic microorganisms on crude oils at elevated temperatures and pressures; Quarterly report, January 1, 1990--March 31, 1990  

SciTech Connect (OSTI)

The objective of this program is to determine the chemical and physical effects of thermophilic organisms on crude oils and cores at elevated temperatures and pressures. Ultimately a data base will be generated which will be used in technical and economic feasibility studies leading to field application. Results of the biotreatment of the teapot Naval Petroleum Resume {number_sign}3(PR3) with BNL strain BNL-4-24 at 65{degree}C under 2000 psi of nitrogen and 80 psi of carbon dioxide are presented. Results are also briefly discussed on the biotreatment of Wilmington, CA crude with BNL-4-22 on the reduction of nickel porphyrin complex. 4 refs., 6 figs.

Premuzic, E.T.; Lin, M.S.

1990-03-01T23:59:59.000Z

116

Efficient degradation of lignocellulosic plant biomass without pretreatment by the 9 thermophilic anaerobe, Anaerocellum thermophilum DSM 6725  

SciTech Connect (OSTI)

Very few cultivated microorganisms can degrade lignocellulosic biomass without chemical pretreatment. We show here that 'Anaerocellum thermophilum' DSM 6725, an anaerobic bacterium that grows optimally at 75 C, efficiently utilizes various types of untreated plant biomass, as well as crystalline cellulose and xylan. These include hardwoods such as poplar, low-lignin grasses such as napier and Bermuda grasses, and high-lignin grasses such as switchgrass. The organism did not utilize only the soluble fraction of the untreated biomass, since insoluble plant biomass (as well as cellulose and xylan) obtained after washing at 75 C for 18 h also served as a growth substrate. The predominant end products from all growth substrates were hydrogen, acetate, and lactate. Glucose and cellobiose (on crystalline cellulose) and xylose and xylobiose (on xylan) also accumulated in the growth media during growth on the defined substrates but not during growth on the plant biomass. A. thermophilum DSM 6725 grew well on first- and second-spent biomass derived from poplar and switchgrass, where spent biomass is defined as the insoluble growth substrate recovered after the organism has reached late stationary phase. No evidence was found for the direct attachment of A. thermophilum DSM 6725 to the plant biomass. This organism differs from the closely related strain A. thermophilum Z-1320 in its ability to grow on xylose and pectin. Caldicellulosiruptor saccharolyticus DSM 8903 (optimum growth temperature, 70 C), a close relative of A. thermophilum DSM 6725, grew well on switchgrass but not on poplar, indicating a significant difference in the biomass-degrading abilities of these two otherwise very similar organisms.

Yang, Sung-Jae [University of Georgia, Athens, GA; Kataeva, Irina [University of Georgia, Athens, GA; Hamilton-Brehm, Scott [ORNL; Engle, Nancy L [ORNL; Tschaplinski, Timothy J [ORNL; Doeppke, Crissa [National Renewable Energy Laboratory (NREL); Davis, Dr. Mark F. [National Renewable Energy Laboratory (NREL); Westpheling, Janet [University of Georgia, Athens, GA; Adams, Michael W. W. [University of Georgia, Athens, GA

2009-01-01T23:59:59.000Z

117

Continuous Thermophilic Composting  

Science Journals Connector (OSTI)

...between 120 and 160 F. MATERIALS AND METHODS The composting...Figure 1 is a sectional diagram of the pilot plant and...addition and removal of material. During operation...problems connected with materials handling, and the value of the...

K. L. Schulze

1962-03-01T23:59:59.000Z

118

Complete genome sequence of Nitrosomonas sp. Is79, an ammonia oxidizing bacterium adapted to low ammonium concentrations  

SciTech Connect (OSTI)

Nitrosomonas sp. Is79 is a chemolithoautotrophic ammonia-oxidizing bacterium that belongs to the family Nitrosomonadaceae within the phylum Proteobacteria. Ammonia oxidation is the first step of nitrification, an important process in the global nitrogen cycle ultimately resulting in the production of nitrate. Nitrosomonas sp. Is79 is an ammonia oxidizer of high interest because it is adapted to low ammonium and can be found in freshwater environments around the world. The 3,783,444-bp chromosome with a total of 3,553 protein coding genes and 44 RNA genes was sequenced by the DOE-Joint Genome Institute Program CSP 2006.

Bollmann, Annette [Miami University, Oxford, OH; Sedlacek, Christopher J [Miami University, Oxford, OH; Laanbroek, Hendrikus J [Netherlands Institute of Ecology (NIOO-KNAW); Suwa, Yuichi [Chuo University, Tokyo, Japan; Stein, Lisa Y [University of California, Riverside; Klotz, Martin G [University of Louisville, Louisville; Arp, D J [Oregon State University; Sayavedra-Soto, LA [Oregon State University; Lu, Megan [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Szeto, Ernest [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Peters, Lin [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL)

2013-01-01T23:59:59.000Z

119

Analytical solutions to the free vibration of a double-walled carbon nanotube carrying a bacterium at its tip  

SciTech Connect (OSTI)

We calculate the natural frequencies and mode shapes of a cantilevered double-walled carbon nanotube carrying a rigid body—representative of a bacterium or virus—at the tip of the outer nanotube. By idealizing the nanotubes as Bernoulli-Euler beams, we are able to obtain exact expressions for both the mode shapes and characteristic frequency equation. Separate analyses are performed for the special case of a concentrated tip mass and the more complicated situation where the tip body also exhibits inertia and mass center offset from the beam tip.

Storch, Joel A. [Department of Mechanical Engineering, California State University, Northridge, CA 91330-8348 (United States); Elishakoff, Isaac [Department of Ocean and Mechanical Engineering, Florida Atlantic University, Boca Raton, FL 33431-0991 (United States)

2013-11-07T23:59:59.000Z

120

Direct Conversion of Plant Biomass to Ethanol by Engineered Caldicellulosiruptor bescii  

SciTech Connect (OSTI)

Ethanol is the most widely used renewable transportation biofuel in the United States, with the production of 13.3 billion gallons in 2012 [John UM (2013) Contribution of the Ethanol Industry to the Economy of the United States]. Despite considerable effort to produce fuels from lignocellulosic biomass, chemical pretreatment and the addition of saccharolytic enzymes before microbial bioconversion remain economic barriers to industrial deployment [Lynd LR, et al. (2008) Nat Biotechnol 26(2):169-172]. We began with the thermophilic, anaerobic, cellulolytic bacterium Caldicellulosiruptor bescii, which efficiently uses unpretreated biomass, and engineered it to produce ethanol. Here we report the direct conversion of switchgrass, a nonfood, renewable feedstock, to ethanol without conventional pretreatment of the biomass. This process was accomplished by deletion of lactate dehydrogenase and heterologous expression of a Clostridium thermocellum bifunctional acetaldehyde/alcohol dehydrogenase. Whereas wild-type C. bescii lacks the ability to make ethanol, 70% of the fermentation products in the engineered strain were ethanol [12.8 mM ethanol directly from 2% (wt/vol) switchgrass, a real-world substrate] with decreased production of acetate by 38% compared with wild-type. Direct conversion of biomass to ethanol represents a new paradigm for consolidated bioprocessing, offering the potential for carbon neutral, cost-effective, sustainable fuel production.

Chung, Daehwan [University of Georgia, Athens, GA; Cha, Minseok [University of Georgia, Athens, GA; Guss, Adam M [ORNL; Westpheling, Janet [University of Georgia, Athens, GA

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Adaptation of the Wine Bacterium Oenococcus oeni to Ethanol Stress: Role of the Small Heat Shock Protein Lo18 in Membrane Integrity  

Science Journals Connector (OSTI)

...the Wine Bacterium Oenococcus oeni to Ethanol Stress: Role of the Small Heat Shock...stressful environment for bacteria because ethanol is a toxic compound that impairs the integrity...O. oeni grown in the presence of 8% ethanol (here, ethanol liposomes), one was...

Magali Maitre; Stéphanie Weidmann; Florence Dubois-Brissonnet; Vanessa David; Jacques Covès; Jean Guzzo

2014-02-28T23:59:59.000Z

122

Genome sequence of the thermophilic fresh-water bacterium Spirochaeta caldaria type strain (H1T), reclassification of Spirochaeta caldaria, Spirochaeta stenostrepta, and Spirochaeta zuelzerae in the genus Treponema as Treponema caldaria comb. nov., Treponema stenostrepta comb. nov., and Treponema zuelzerae comb. nov., and emendation of the genus Tr  

SciTech Connect (OSTI)

Spirochaeta caldaria Pohlschroeder et al. 1995 is an obligately anaerobic, spiral-shaped bac- terium that is motile via periplasmic flagella. The type strain, H1T, was isolated in 1990 from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA, and is of in- terest because it enhances the degradation of cellulose when grown in co-culture with Clos- tridium thermocellum. Here we provide a taxonomic re-evaluation for S. caldaria based on phylogenetic analyses of 16S rRNA sequences and whole genomes, and propose the reclassi- fication of S. caldaria and two other Spirochaeta species as members of the emended genus Treponema. Whereas genera such as Borrelia and Sphaerochaeta possess well-distinguished genomic features related to their divergent lifestyles, the physiological and functional ge- nomic characteristics of Spirochaeta and Treponema appear to be intermixed and are of little taxonomic value. The 3,239,340 bp long genome of strain H1T with its 2,869 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Abt, Birte [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Scheuner, Carmen [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Han, Cliff [Los Alamos National Laboratory (LANL); Lu, Megan [Los Alamos National Laboratory (LANL); Misra, Monica [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Gronow, Sabine [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2013-01-01T23:59:59.000Z

123

Isolation, purification and spectrometric analysis of PSP toxins from moraxella sp., a bacterium associated with a toxic dinoflagellate  

SciTech Connect (OSTI)

Paralytic shellfish poisoning (PSP) is a seafood intoxication syndrome caused by the injestion of shellfish contaminated with toxins produced by algae known as dinoflagellates. The PSP toxins, saxitoxin and its derivatives, act to block voltage-dependent sodium channels and can cause paralysis and even death at higher doses. It is well documented that bacteria coexist with many harmful or toxic algal species, though the exact nature of the association in relation to toxin production is unknown. Recently, the bacterium Moraxella sp. was isolated from the PSP toxin producing dinoflagellate Alexandrium tamarense. Through HPLC analysis and saxitoxin receptor binding assays performed on crude bacterial extracts, it appears that Moraxella sp. is capable of producing saxitoxin and several of its derivatives. However, physical confirmation (e.g. mass spectrometry) of these results is still needed.

Boyce, S.D.; Doucette, G.J.

1994-12-31T23:59:59.000Z

124

Reduction of Cr(VI) under acidic conditions by the facultative Fe(III)-reducing bacterium Acidiphilium cryptum  

SciTech Connect (OSTI)

The potential for biological reduction of Cr(VI) under acidic conditions was evaluated with the acidophilic, facultatively metal-reducing bacterium Acidiphilium cryptum strain JF-5 to explore the role of acidophilic microorganisms in the Cr cycle in low-pH environments. An anaerobic suspension of washed A. cryptum cells rapidly reduced 50 M Cr(VI) at pH 3.2; biological reduction was detected from pH 1.7-4.7. The reduction product, confirmed by XANES analysis, was entirely Cr(III) that was associated predominantly with the cell biomass (70-80%) with the residual residing in the aqueous phase. Reduction of Cr(VI) showed a pH optimum similar to that for growth and was inhibited by 5 mM HgCl2, suggesting that the reaction was enzyme-mediated. Introduction of O2 into the reaction medium slowed the reduction rate only slightly, whereas soluble Fe(III) (as ferric sulfate) increased the rate dramatically, presumably by the shuttling of electrons from bioreduced Fe(II) to Cr(VI) in a coupled biotic-abiotic cycle. Starved cells could not reduce Cr(VI) when provided as sole electron acceptor, indicating that Cr(VI) reduction is not an energy-conserving process in A. cryptum. We speculate, rather, that Cr(VI) reduction is used here as a detoxification mechanism.

David E. Cummings; Scott Fendorf; Rajesh K. Sani; Brent M. Peyton; Timothy S. Magnuson

2007-01-01T23:59:59.000Z

125

Methanogens and the diversity of archaebacteria.  

Science Journals Connector (OSTI)

...mesophilic methanogen isolated from marine sediments. Appl. Environ...ization of a thermophilic marine methanogenic bacterium, Methanogenium...Methanogenium, a new genus of marine methanogenic bacteria, and...Stupperich, and G. Fuchs. 1985. Detection of acetyl coenzyme A as an...

W J Jones; D P Nagle Jr; W B Whitman

1987-03-01T23:59:59.000Z

126

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

0, 0, p. 3545-3553 Vol. 76, No. 11 0099-2240/10/$12.00 doi:10.1128/AEM.02689-09 Copyright © 2010, American Society for Microbiology. All Rights Reserved. Diversity of Bacteria and Glycosyl Hydrolase Family 48 Genes in Cellulolytic Consortia Enriched from Thermophilic Biocompost ᰔ Javier A. Izquierdo, 1,2 Maria V. Sizova, 1,2 † and Lee R. Lynd 1,2 * Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, and BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 Received 4 November 2009/Accepted 30 March 2010 The enrichment from nature of novel microbial communities with high cellulolytic activity is useful in the identification of novel organisms and novel functions that enhance the fundamental understanding of micro- bial cellulose degradation. In this work we identify predominant organisms in three cellulolytic

127

CONTRIBUTION OF PROTOZOA TO THE RUMEN CELLULOLYTIC ACTIVITY  

E-Print Network [OSTI]

and embedded in Epon. Ultrathin sections were stained in uranyl ace- tate and postained in lead nitrate

Paris-Sud XI, Université de

128

Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome To Date  

SciTech Connect (OSTI)

Here we describe the genome of Mesotoga prima MesG1.Ag4.2, the first genome of a mesophilic Thermotogales bacterium. Mesotoga prima was isolated from a polychlorinated biphenyl (PCB)-dechlorinating enrichment culture from Baltimore Harbor sediments. Its 2.97 Mb genome is considerably larger than any previously sequenced Thermotogales genomes, which range between 1.86 and 2.30 Mb. This larger size is due to both higher numbers of protein-coding genes and larger intergenic regions. In particular, the M. prima genome contains more genes for proteins involved in regulatory functions, for instance those involved in regulation of transcription. Together with its closest relative, Kosmotoga olearia, it also encodes different types of proteins involved in environmental and cell-cell interactions as compared with other Thermotogales bacteria. Amino acid composition analysis of M. prima proteins implies that this lineage has inhabited low-temperature environments for a long time. A large fraction of the M. prima genome has been acquired by lateral gene transfer (LGT): a DarkHorse analysis suggests that 766 (32%) of predicted protein-coding genes have been involved in LGT after Mesotoga diverged from the other Thermotogales lineages. A notable example of a lineage-specific LGT event is a reductive dehalogenase gene - a key enzyme in dehalorespiration, indicating M. prima may have a more active role in PCB dechlorination than was previously assumed.

Zhaxybayeva, Olga [Dartmouth College; Swithers, Kristen S [University of Connecticut, Storrs; Foght, Julia [University of Alberta, Edmondton, Canada; Green, Anna G. [University of Connecticut; Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Teshima, Hazuki [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Dlutek, Marlena [Dalhousie University, Halifax, Nova Scotia, CANADA; Doolittle, W. Ford [Dalhousie University, Halifax, Nova Scotia, CANADA; Noll, Kenneth M [University of Connecticut, Storrs; Nesbo, Camilla [University of Oslo, Norway

2012-01-01T23:59:59.000Z

129

Phylogeography of the Thermophilic Cyanobacterium Mastigocladus laminosus  

Science Journals Connector (OSTI)

...11/8/9) Group 5 CCMEE 5268 Chena Hot Springs, AK 19 (4/6/9) Group 6 CCMEE 5319 Hot spring near Lake Amatitlan, Guatemala 20 (8/9/10) CCMEE 5267 Chena Hot Springs, AK 21 (9/7/10) CCMEE 5318...

Scott R. Miller; Richard W. Castenholz; Deana Pedersen

2007-06-08T23:59:59.000Z

130

Chromosome arrangement within a bacterium  

E-Print Network [OSTI]

Background: The contour length of the circular chromosome of bacteria is greater than a millimeter but must be accommodated within a cell that is only a few micrometers in length. Bacteria do not have nucleosomes and little ...

Teleman, Aurelio A.

131

JOURNAL OF BACTERIOLOGY, July 2008, p. 45684575 Vol. 190, No. 13 0021-9193/08/$08.00 0 doi:10.1128/JB.00369-08  

E-Print Network [OSTI]

. The thermophilic bacterium Thermus thermophilus, with a growth temperature ranging from 45° to 85°C, belongs to one). The chromosomes are highly conserved with an identity of 94%, but variations are found, predominantly in cell), and the integral membrane components, called TpsB, of the two

Kleinschmidt, Jörg H.

132

deb_pone.0062881 1..10  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Construction Construction of a Stable Replicating Shuttle Vector for Caldicellulosiruptor Species: Use for Extending Genetic Methodologies to Other Members of This Genus Daehwan Chung 1,2 , Minseok Cha 1,2 , Joel Farkas 1,2¤ , Janet Westpheling 1,2 * 1 Department of Genetics, University of Georgia, Athens, Georgia, United States of America, 2 The BioEnergy Science Center, Department of Energy, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America Abstract The recalcitrance of plant biomass is the most important barrier to its economic conversion by microbes to products of interest. Thermophiles have special advantages for biomass conversion and members of the genus Caldicellulosiruptor are the most thermophilic cellulolytic microbes known. In this study, we report the construction of a replicating shuttle vector for Caldicellulosiruptor species based on

133

Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Overcoming Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement Daehwan Chung 1,2 , Joel Farkas 1,2 and Janet Westpheling 1,2* Abstract Background: Thermophilic microorganisms have special advantages for the conversion of plant biomass to fuels and chemicals. Members of the genus Caldicellulosiruptor are the most thermophilic cellulolytic bacteria known. They have the ability to grow on a variety of non-pretreated biomass substrates at or near ~80°C and hold promise for converting biomass to bioproducts in a single step. As for all such relatively uncharacterized organisms with desirable traits, the ability to genetically manipulate them is a prerequisite for making them useful. Metabolic engineering of pathways for product synthesis is relatively simple compared to engineering the ability to utilize

134

Use of Cellulolytic Marine Bacteria for Enzymatic Pretreatment in Microalgal Biogas Production  

Science Journals Connector (OSTI)

...digestion is an alternative to previous...30C), energy costs were...prospect for biofuels. Bioresour...disruption for biofuel development. Appl. Energy 91 :116-121...microalgae for biofuels production...of micro-algae. Ph.D...

Camilo Muñoz; Catalina Hidalgo; Manuel Zapata; David Jeison; Carlos Riquelme; Mariella Rivas

2014-05-02T23:59:59.000Z

135

E-Print Network 3.0 - acidothermus cellulolytics atcc Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

major clade of prokaryotes with ancient adaptations to life on land Summary: , Himmel M, Leighton L, Updegraff DM. 1986. Isolation and characterization of acidothermus......

136

Insertion of Endocellulase Catalytic Domains into Thermostable Consensus Ankyrin Scaffolds: Effects on Stability and Cellulolytic Activity  

Science Journals Connector (OSTI)

...of these microbes produce a battery of secreted cellulases with...consensus ankyrin protein, the thermal denaturation midpoint (Tm...enhancements. We determined the thermal denaturation of these constructs...averaging five wavelength scans. Thermal unfolding transitions were...

Eva S. Cunha; Christine L. Hatem; Doug Barrick

2013-08-23T23:59:59.000Z

137

Use of Cellulolytic Marine Bacteria for Enzymatic Pretreatment in Microalgal Biogas Production  

Science Journals Connector (OSTI)

...inhibit the anaerobic digestion process because of (i) ammonium...Singh, J and S Gu. 2010. Commercialization potential of microalgae for...biodegradability. Chem. Eng. Process. 45 :711-718. doi: 10...yield of methane fermentation process. Pol. J. Environ. Stud...

Camilo Muñoz; Catalina Hidalgo; Manuel Zapata; David Jeison; Carlos Riquelme; Mariella Rivas

2014-05-02T23:59:59.000Z

138

Pathogenesis of the carcinogenic bacterium, Helicobacter pylori  

E-Print Network [OSTI]

Gastric cancer is the second most common malignancy in the digestive system and the second leading cause of cancer-related death worldwide. Epidemiological data and experimental studies have identified several risk factors ...

Lee, Chung-Wei, Ph. D. Massachusetts Institute of Technology

2007-01-01T23:59:59.000Z

139

Genome Streamlining in a Cosmopolitan Oceanic Bacterium  

Science Journals Connector (OSTI)

...no evidence of DNA originating from recent horizontal gene transfer events. The presence of DNA uptake and competence genes (PilC, PilD, PilE, PilF, PilG, PilQ, comL, and cinA) in the genome suggests that P. ubique has the ability to acquire foreign...

Stephen J. Giovannoni; H. James Tripp; Scott Givan; Mircea Podar; Kevin L. Vergin; Damon Baptista; Lisa Bibbs; Jonathan Eads; Toby H. Richardson; Michiel Noordewier; Michael S. Rappé; Jay M. Short; James C. Carrington; Eric J. Mathur

2005-08-19T23:59:59.000Z

140

Cellulose- and Xylan-Degrading Thermophilic Anaerobic Bacteria from Biocompost  

Science Journals Connector (OSTI)

...to utilize both cellulose and xylan, including strains of Clostridium...species reported to utilize xylan, and the rate of cellulose...able to degrade cellulose, xylan, and their mixture with the...locations 40 to 50 cm below the surface and temperatures ranging from...

M. V. Sizova; J. A. Izquierdo; N. S. Panikov; L. R. Lynd

2011-02-11T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Thermophilic blue-green algae and the thermal environment.  

Science Journals Connector (OSTI)

...fairly constant during the exponential phase of growth in 0. terebriformis, with...enoid values for the exponential growth phase varied inversely with the light intensity...53. Castenholz, R. W. 1968. The behavior of Oscillatoria terebri- formis in hot...

R W Castenholz

1969-12-01T23:59:59.000Z

142

Kinetics of inactivation of indicator pathogens during thermophilic anaerobic digestion  

E-Print Network [OSTI]

relationships are necessary. ª 2010 Elsevier Ltd. All rights reserved. 1. Introduction Land application the opportunity to put sewage sludge, which otherwise needs to be disposed of, towards beneficial use such as crop, enteric viruses and Salmonella spp. Land application of Class B biosolids, which require only reduction

143

Glycoside Hydrolase Activities of Thermophilic Bacterial Consortia Adapted to Switchgrass  

Science Journals Connector (OSTI)

...configurations designed to transform lignocellulosic biomass into biofuels (11, 23). The large variety of potential biomass feedstocks and pretreatments available require tailored glycoside hydrolase cocktails that function optimally under diverse...

John M. Gladden; Martin Allgaier; Christopher S. Miller; Terry C. Hazen; Jean S. VanderGheynst; Philip Hugenholtz; Blake A. Simmons; Steven W. Singer

2011-07-01T23:59:59.000Z

144

Thermophilic blue-green algae and the thermal environment.  

Science Journals Connector (OSTI)

...partly derived from magmatic steam, although an ultimate meteoric...common types of thermal waters (based on origin and chemistry) as...partly derived from magmatic steam. The princi- pal solutes are...upper limit with parentheses is based on certain observations of occurrence...

R W Castenholz

1969-12-01T23:59:59.000Z

145

Thermophilic blue-green algae and the thermal environment.  

Science Journals Connector (OSTI)

...since little is known about the natural energy or carbon sources for bacteria that grow...ranging from about 55 to 40 C. The specific energy and carbon sources for growth are unknown...primary producers are probably the main energy sources available to the bacteria, although...

R W Castenholz

1969-12-01T23:59:59.000Z

146

Conversion of sugarcane bagasse to carboxylic acids under thermophilic conditions  

E-Print Network [OSTI]

?????????????. 17 1.4 Project description????????????..?... 25 II MATERIALS AND METHODS???????????? 27 2.1 Biomass feedstock??????????????. 27 2.2 Biomass pretreatment????????????? 29 2.3 Fermentation material and methods.... This is followed by introducing promising lignocellulosic biomass feedstocks and challenges in lignocellulosic biomass conversion. Subsequently, it presents the process description and recent advances of the MixAlco process, a novel and promising biomass...

Fu, Zhihong

2009-05-15T23:59:59.000Z

147

Microbial Ecology of Thermophilic Anaerobic Digestion. Final Report  

DOE R&D Accomplishments [OSTI]

This grant supported research on methanogenic archaea. The two major areas that were supported were conversion of acetic acid to methane and nitrogen fixation by Methanosarcina. Among the achievements of this research were the isolation of novel methanogenic cultures, elucidation of the pathways from acetate to methane, description of a specific DNA-binding complex in nitrogen fixing methanogens, and demonstration of an alternative nitrogenase in Methanosarcina.

Zinder, Stephen H.

2000-04-15T23:59:59.000Z

148

Fermentation method producing ethanol  

DOE Patents [OSTI]

Ethanol is the major end product of an anaerobic, thermophilic fermentation process using a mutant strain of bacterium Clostridium thermosaccharolyticum. This organism is capable of converting hexose and pentose carbohydrates to ethanol, acetic and lactic acids. Mutants of Clostridium thermosaccharolyticum are capable of converting these substrates to ethanol in exceptionally high yield and with increased productivity. Both the mutant organism and the technique for its isolation are provided.

Wang, Daniel I. C. (Belmont, MA); Dalal, Rajen (Chicago, IL)

1986-01-01T23:59:59.000Z

149

Final Technical Report  

SciTech Connect (OSTI)

This grant provided the basic funding that enabled me to carry out a detailed characterization of the proteins used by the aerobic soil bacterium, Thermobifida fusca, to degrade cellulose and to study the mechanisms used by T. fusca to regulate cellulase synthesis. This work resulted in 53 publications and led to the decision by The DOE Joint Genome Institute to sequence the T. fusca genome. T. fusca is now recognized as one of the best studied cellulolytic microorganisms and our work led to the discovery of a novel class of cellulases, processive endoglucanases, which are found in many cellulolytic bacteria including both aerobes and anaerobes. In addition, we were able to determine the mechanism by which Cel9A caused processive hydrolysis of cellulose. This research also helped to explain why many cellulolytic microorganisms produce two different exocellulases, as we showed that these enzymes have different specificities, with one attacking the reducing end of a cellulose chain and the other attacking the nonreducing end. Our work also provided additional evidence for the importance of a cellulose binding domain (carbohydrate binding module) [CBM] in the hydrolysis of crystalline cellulose.

David B. Wilson

2008-04-02T23:59:59.000Z

150

Effect of Lignin Removal by Alkaline Peroxide Pretreatment on the Susceptibility of Corn Stover to Purified Cellulolytic and Xylanolytic Enzymes  

SciTech Connect (OSTI)

Pretreatment of corn stover with alkaline peroxide (AP) at pH 11.5 resulted in reduction of lignin content in the residual solids as a function of increasing batch temperature. Scanning electron microscopy of these materials revealed notably more textured surfaces on the plant cell walls as a result of the delignifying pretreatment. As expected, digestion of the delignified samples with commercial cellulase preparations showed an inverse relationship between the content of lignin present in the residual solids after pretreatment and the extent of both glucan and xylan conversion achievable. Digestions with purified enzymes revealed that decreased lignin content in the pretreated solids did not significantly impact the extent of glucan conversion achievable by cellulases alone. Not until purified xylanolytic activities were included with the cellulases were significant improvements in glucan conversion realized. In addition, an inverse relationship was observed between lignin content after pretreatment and the extent of xylan conversion achievable in a 24-h period with the xylanolytic enzymes in the absence of the cellulases. This observation, coupled with the direct relationship between enzymatic xylan and glucan conversion observed in a number of cases, suggests that the presence of lignins may not directly occlude cellulose present in lignocelluloses but rather impact cellulase action indirectly by its association with xylan.

Selig, M. J.; Vinzant, T. B.; Himmel, M. E.; Decker, S. R.

2009-01-01T23:59:59.000Z

151

Regulation of Expression of Cellulosomes and Noncellulosomal (Hemi)Cellulolytic Enzymes in Clostridium cellulovorans during Growth on Different Carbon Sources  

Science Journals Connector (OSTI)

...improved process for isolation of corn fiber gum. Cereal Chem. 75: 408-411...Isolation of hemicellulose from corn fiber by alkaline hydrogen peroxide...properties of arabinoxylans from discrete corn wet-milling fiber fractions. J. Agric. Food...

Sung Ok Han; Hee-Yeon Cho; Hideaki Yukawa; Masayuki Inui; Roy H. Doi

2004-07-01T23:59:59.000Z

152

Purification and Characterization of Exo-?-d-Glucosaminidase from a Cellulolytic Fungus,Trichoderma reesei PC-3-7  

Science Journals Connector (OSTI)

...against completely deacetylated chitosan (chitosan 10B). It apparently exhibited...proteins containing soybean trypsin inhibitor (20 kDa), carbonic anhydrase...of 4.0 for the hydrolysis of chitosan 10B (Fig.2). The enzyme...

Masahiro Nogawa; Hiroya Takahashi; Aya Kashiwagi; Kenji Ohshima; Hirofumi Okada; Yasushi Morikawa

1998-03-01T23:59:59.000Z

153

November 19, 2011 1  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

9, 2011 1 9, 2011 1 2 S-layer homology (SLH) domain proteins Csac_0678 and Csac_2722 implicated in 3 plant polysaccharide deconstruction by the extremely thermophilic bacterium 4 Caldicellulosiruptor saccharolyticus 5 6 Inci Ozdemir, Sara E. Blumer-Schuette, and Robert M. Kelly* 7 8 Department of Chemical and Biomolecular Engineering, 9 North Carolina State University, Raleigh, NC 27695-7905 10 11 12 Submitted to: Applied and Environmental Microbiology (November, 2011) 13 AEM07031-11 (Revised Version of AEM05791-1) 14 15 Running title: S-layer homology domain proteins in C. saccharolyticus 16 17 Keywords: Caldicellulosiruptor saccharolyticus, extreme thermophile, plant biomass, 18 glycoside hydrolases, S-layer homology domains 19 20 21 *Address correspondence to: Robert M. Kelly 22

154

E-Print Network 3.0 - acidophilic bacterium acidithiobacillus...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Moscow State University Collection: Biology and Medicine 52 Multiple Mechanisms of Uranium Immobilization by Cellulomonas sp. Strain ES6 Summary: released by cells. Cultures...

155

Genome analysis of the Anerobic Thermohalophilic bacterium Halothermothrix orenii  

E-Print Network [OSTI]

and expression of an alpha- amylase gene, amyA, from thepolyextremophilic alpha-amylase AmyB from Halothermothrixand sugar processing (alpha amylases, beta glucosidase) and

Mavromatis, Konstantinos

2009-01-01T23:59:59.000Z

156

E-Print Network 3.0 - antarctica bacterium pseudoalteromonas...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Microbiology and Blackwell Publishing Ltd Summary: % sequence similarity with the CFB group. The main genera of gggg-Proteobacteria were Pseudoalteromonas (14... Vibrio and...

157

Presence of an Unusual Methanogenic Bacterium in Coal Gasification Waste  

Science Journals Connector (OSTI)

...waste were enriched in a mineral salts medium containing hydrogen and acetate as potential...Transfer of the enrichments to methanol medium resulted in the initial growth of a strain...waste were enriched in a mineral salts medium containing hydrogen and acetate as potential...

Francisco A. Tomei; Dwight Rouse; James S. Maki; Ralph Mitchell

1988-12-01T23:59:59.000Z

158

Presence of an Unusual Methanogenic Bacterium in Coal Gasification Waste  

Science Journals Connector (OSTI)

...stoppered bottle and degassing the media at least three times with 80% N2-20% CO2. The media were then reduced with the...using 0.22-p,m- pore-size Millex-GV filter units...autoclave. By equilibrating the media with 0.4 atm (ca. 40...

Francisco A. Tomei; Dwight Rouse; James S. Maki; Ralph Mitchell

1988-12-01T23:59:59.000Z

159

Growth of Bacterium coli and Staphylococcus albus in Heavy Water  

Science Journals Connector (OSTI)

... , prepared by redissolving lyophilized aqueous nutrient broth in 99-8 per cent heavy water (Norsk Hydro), was compared with their growth in aqueous medium and in medium with various ...

ELIZABETH VAN HORN; G. C. WARE

1959-09-12T23:59:59.000Z

160

The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens  

E-Print Network [OSTI]

gas produced in the rumen is CO2 (65.5 mol%), yet the metabolic characteristics of capnophilic (CO2 acid is a major VFA constituting 50%­60% of the total1. A major gas present in the rumen is CO2 (65 acceptor. Genome-scale metabolic flux analysis indicated that CO2 is important for the carboxylation

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

Hydrogen Production by the Photosynthetic Bacterium Rhodospirillum rubrum  

Science Journals Connector (OSTI)

...00/0 Hydrogen Production by the Photosynthetic...Continuous photosynthetic production ofhydrogen by Rhodospirillum...dry weight) of cells with whey as a hydrogen...processing specific organic wastes could be...in large-scale production ofhydrogen together...organisms that can use solar energy offer several...

Hans Zürrer; Reinhard Bachofen

1979-05-01T23:59:59.000Z

162

Physiological Ecology of a Gliding Bacterium Containing Bacteriochlorophyll a  

Science Journals Connector (OSTI)

...at high levels by NIR radiation, which is absorbed...phylogenetic relation- ship to other phototrophs...bacteriochlorophyll and carotenoids in marine bacteria. Plant Cell...M. Howard. 1972. Detection of bacterio- chlorophyll-containing...Hollaender (ed.), Radiation biology. McGraw-Hi4...

Beverly K. Pierson; Stephen J. Giovannoni; Richard W. Castenholz

1984-03-01T23:59:59.000Z

163

Genetic manipulation of the obligate chemolithoautotrophic bacterium Thiobacillus denitrificans  

E-Print Network [OSTI]

water  (18-­?M?  resistance)  obtained  from  a  Milli-­?Q  Biocel  system  (Millipore,   Bedford,  MA)  or  similar  purification  system  

Beller, H.R.

2012-01-01T23:59:59.000Z

164

Genetic manipulation of the obligate chemolithoautotrophic bacterium Thiobacillus denitrificans  

E-Print Network [OSTI]

uranium-­?contaminated  aquifers  by   in  situ   reductive  immobilization  [i.e. ,  microbially   mediated  conversion  

Beller, H.R.

2012-01-01T23:59:59.000Z

165

Ultrastructural Changes in an Obligately Barophilic Marine Bacterium after Decompression  

Science Journals Connector (OSTI)

...for several reasons. First, sensitivity to warming...temperature-pressure regimes of the oceans. Third, warming (19...decompression. (A) Low-power scan (bar, 2 ~Lm...kinetics of inactivation were first order for the first few hours of exposure...

Roger A. Chastain; A. Aristides Yayanos

1991-05-01T23:59:59.000Z

166

Fermentation and Electrohydrogenic Approaches to Hydrogen Production - DOE Hydrogen and Fuel Cells Program FY 2012 Annual Progress Report  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

8 8 DOE Hydrogen and Fuel Cells Program FY 2012 Annual Progress Report Pin-Ching Maness (Primary Contact), Katherine Chou, and Lauren Magnusson National Renewable Energy Laboratory (NREL) 15013 Denver West Parkway Golden, CO 80401 Phone: (303) 384-6114 Email: pinching.maness@nrel.gov DOE Manager HQ: Eric Miller Phone: (202) 287-5829 Email: Eric.Miller@hq.doe.gov Subcontractor: Bruce Logan, Pennsylvania State University, State College, PA Start Date: October 1, 2004 Projected End Date: Project continuation and direction determined annually by DOE Fiscal Year (FY) 2012 Objectives Optimize sequencing fed-batch parameters in converting * cellulose to hydrogen by the cellulolytic bacterium Clostridium thermocellum; aimed at lowering feedstock cost. Improve plasmid stability in * C. thermocellum; aimed

167

Thermophilic and thermoacidophilic sugar transporter genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods  

DOE Patents [OSTI]

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for transporting sugars across cell membranes using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Thompson, David N; Apel, William A; Thompson, Vicki S; Reed, David W; Lacey, Jeffrey A

2013-11-05T23:59:59.000Z

168

Characterization and Evolution of Tetrameric Photosystem I from the Thermophilic Cyanobacterium Chroococcidiopsis sp TS-821  

Science Journals Connector (OSTI)

...attractive for solar energy applications such...contrast to the disadvantage of having a monomeric...somewhat of an advantage, which is supported...higher light energy conversion efficiency...CP43) dissipate energy in cyanobacteria...

Meng Li; Dmitry A. Semchonok; Egbert J. Boekema; Barry D. Bruce

2014-03-28T23:59:59.000Z

169

Hydrogen Production by the Thermophilic Alga Mastigocladus laminosus: Effects of Nitrogen, Temperature, and Inhibition of Photosynthesis  

Science Journals Connector (OSTI)

...production of hydrogen by solar radiation was also...production of hydrogen by solar radiation was also...Although there are both advantages and disadvantages to this approach...Thus, the total solar energy conversion efficiency...

Kazuhisa Miyamoto; Patrick C. Hallenbeck; John R. Benemann

1979-09-01T23:59:59.000Z

170

High-solids enrichment of thermophilic microbial communities and their enzymes on bioenergy feedstocks  

E-Print Network [OSTI]

Progress and Challenges in Enzyme Development for Biomasscommunities and their enzymes on bioenergy feedstocks AmithaStrain selection, enzyme extraction optimization, and

Reddy, A. P.

2012-01-01T23:59:59.000Z

171

Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods  

DOE Patents [OSTI]

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for transporting sugars across cell membranes using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Thompson, David N. (Idaho Falls, ID); Apel, William A. (Jackson, WY); Thompson, Vicki S. (Idaho Falls, ID); Reed, David W. (Idaho Falls, ID); Lacey, Jeffrey A. (Idaho Falls, ID)

2011-06-14T23:59:59.000Z

172

Phylogenetic Analysis of Bacterial Communities in Mesophilic and Thermophilic Bioreactors Treating Pharmaceutical Wastewater  

Science Journals Connector (OSTI)

...a full-scale industrial wastewater treatment facility consisting...AND METHODS Study site. The wastewater treatment facility consists...exceeding 45C without cell recycling, due to poor bacterial flocculation...oxygen demand of the untreated wastewater has historically varied between...

Timothy M. LaPara; Cindy H. Nakatsu; Lisa Pantea; James E. Alleman

2000-09-01T23:59:59.000Z

173

Characterization and Evolution of Tetrameric Photosystem I from the Thermophilic Cyanobacterium Chroococcidiopsis sp TS-821  

Science Journals Connector (OSTI)

...transmission electron microscopy|TEM, transmission electron microscopy...splitting of water with the reduction...ultracentrifugation (AUC), electron microscopy...a similar treatment was applied...gradient after dialysis and concentration...

Meng Li; Dmitry A. Semchonok; Egbert J. Boekema; Barry D. Bruce

2014-03-28T23:59:59.000Z

174

E-Print Network 3.0 - anaerobic thermophiles annual Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Implementation of Summary: ;12;Abstract Anaerobic digestion of dairy manure produces biogas that can be captured and used for fuel while... offering environmental benefits. Dairy...

175

E-Print Network 3.0 - anaerobic thermophiles final Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

for treating this waste stream since it results in two valuable final products, biogas and ... Source: Columbia University - Waste-to-Energy Research and Technology Council...

176

E-Print Network 3.0 - anaerobic thermophilic digestion Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Resources which provide more detailed infor- mation on anaerobic digesters are listed. Biogas... on the source of the organic matter and the management of the anaerobic digestion...

177

E-Print Network 3.0 - anaerobic thermophiles progress Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

due to various... returns from energy and byproduct sales. Keywords: Anaerobic digestion, biogas, cooperatives, carbon Source: Laughlin, Robert B. - Department of Physics, Stanford...

178

High-solids enrichment of thermophilic microbial communities and their enzymes on bioenergy feedstocks  

E-Print Network [OSTI]

and their enzymes on bioenergy feedstocks Amitha P. ReddyVanderGheynst 1,2* Joint BioEnergy Institute, Emeryville, CA2009. The water footprint of bioenergy. Proceedings of the

Reddy, A. P.

2012-01-01T23:59:59.000Z

179

High-solids enrichment of thermophilic microbial communities and their enzymes on bioenergy feedstocks  

E-Print Network [OSTI]

employing plant biomass conversion to fuels (Gerbens-LeenesLaboratory, 94551; Biomass Science and Conversion Technologybiomass for liquid fuel production. Limiting factors to their utilization include sustainable harvest, transportation, storage and conversion

Reddy, A. P.

2012-01-01T23:59:59.000Z

180

Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass  

Science Journals Connector (OSTI)

...N , C Rohde, C Munk, M Nolan, S Lucas, TG Del Rio, H Tice, S Deshpande, JF Cheng, R Tapia, C Han, L Goodwin, S Pitluck, K Liolios, K Mavromatis, N Mikhailova, A Pati, A Chen, K Palaniappan, M Land, L Hauser, YJ Chang, CD Jeffries...

Stephanie A. Eichorst; Chijioke Joshua; Noppadon Sathitsuksanoh; Seema Singh; Blake A. Simmons; Steven W. Singer

2014-09-26T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Genome sequence of the moderately thermophilic halophile Flexistipes sinusarabici strain (MAS10T)  

SciTech Connect (OSTI)

Flexistipes sinusarabici Fiala et al. 2000 is the type species of the genus Flexistipes in the fami- ly Deferribacteraceae. The species is of interest because of its isolated phylogenetic location in a genomically under-characterized region of the tree of life, and because of its origin from a multiply extreme environment; the Atlantis Deep brines of the Red Sea, where it had to struggle with high temperatures, high salinity, and a high concentrations of heavy metals. This is the fourth completed genome sequence to be published of a type strain of the family Deferribacteraceae. The 2,526,590 bp long genome with its 2,346 protein-coding and 53 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Abt, Birte [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute

2011-01-01T23:59:59.000Z

182

Thermophilic and thermoacidophilic metabolism genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods  

DOE Patents [OSTI]

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Thompson, Vicki S; Apel, William A; Reed, David W; Lee, Brady D; Thompson, David N; Roberto, Francisco F; Lacey, Jeffrey A

2014-05-20T23:59:59.000Z

183

Hydrogen Production by the Thermophilic Alga Mastigocladus laminosus: Effects of Nitrogen, Temperature, and Inhibition of Photosynthesis  

Science Journals Connector (OSTI)

...production of hydrogen by solar radiation was also demonstrated...low-cost culture and H2 collector system, as well as...using specially designed collectors, the heat captured...heat. Thus, the total solar energy conversion efficiency...at a 650 angle to the horizontal and facing southwest...

Kazuhisa Miyamoto; Patrick C. Hallenbeck; John R. Benemann

1979-09-01T23:59:59.000Z

184

Ethanol production by thermophilic bacteria: metabolic control of end product formation in Thermoanaerobium brockii.  

Science Journals Connector (OSTI)

...effects of exogenous hydrogen on glucose fermentation...direct consequence of hydrogen consumption by the methanogen...phosphoroclastic activity of cell extracts in that H2...presence of exogenous hydrogen was associated with inhibition...bacteria for chemical and fuel production neces- sitates...

A Ben-Bassat; R Lamed; J G Zeikus

1981-04-01T23:59:59.000Z

185

Isolation from soil and properties of the extreme thermophile Clostridium thermohydrosulfuricum.  

Science Journals Connector (OSTI)

...meadow)' 108 JWlll Straw compost' 120 JW112 Soil near a hot pipeline" 156 JW113 Forest soil" 105 JW120 Mud from alkaline hot spring...fraction of the bases in the DNA are being modified in some wax. DISCUSSION In this report, we have rlescribe(i the isolation...

J Wiegel; L G Ljungdahl; J R Rawson

1979-09-01T23:59:59.000Z

186

Characterization and Evolution of Tetrameric Photosystem I from the Thermophilic Cyanobacterium Chroococcidiopsis sp TS-821  

Science Journals Connector (OSTI)

...Knoxville, Tennessee 37996 c Department of Electron Microscopy, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands d Department of Microbiology, University of Tennessee...

Meng Li; Dmitry A. Semchonok; Egbert J. Boekema; Barry D. Bruce

2014-03-28T23:59:59.000Z

187

2216 Biophysical Journal Volume 84 April 2003 22162222 Electrostatic Contributions to the Stability of a Thermophilic  

E-Print Network [OSTI]

caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-Csp. For 27 mutations that narrow the difference in sequence between Bc-Csp and Bs-CspB, calculated changes in unfolding free energy (DG) and experimental results have a correlation coefficient of 0.98. Bc-Csp appears

Weston, Ken

188

Synergism of Glycoside Hydrolase Secretomes from Two Thermophilic Bacteria Cocultivated on Lignocellulose  

Science Journals Connector (OSTI)

...carbohydrate-binding modules (CBMs). INTRODUCTION Global warming, the energy crisis, and health...of carbon flux on both local and global scales. Understanding of cellulose...2011BAD22B02-01), and China Risun Coal Chemicals Group Ltd. REFERENCES...

Kundi Zhang; Xiaohua Chen; Wolfgang H. Schwarz; Fuli Li

2014-02-14T23:59:59.000Z

189

Process for Generation of Hydrogen Gas from Various Feedstocks Using Thermophilic Bacteria  

SciTech Connect (OSTI)

A method for producing hydrogen gas is provided comprising selecting a bacteria from the Order Thermotogales, subjecting the bacteria to a feedstock and to a suitable growth environment having an oxygen concentration below the oxygen concentration of water in equilibrium with air; and maintaining the environment at a predetermined pH and at a temperature of at least approximately 45 degrees C. for a time sufficient to allow the bacteria to metabolize the feedstock.

Ooteghem Van, Suellen

2005-09-13T23:59:59.000Z

190

Thermophilic Fe(III)-Reducing Bacteria from the Deep Subsurface: The Evolutionary Implications  

Science Journals Connector (OSTI)

...233 ( 1991 ); B. N. Bjornstad et al.,Ground Water Monitoring Remediation 14, 140 (1994). 12 R...1991) ; B.-N.-Bjornstad et al., Ground Water Monitoring Remediation 14, 140-(1994). R. G. Arnold, T...

Shi V. Liu; Jizhong Zhou; Chuanlun Zhang; David R. Cole; M. Gajdarziska-Josifovska; Tommy J. Phelps

1997-08-22T23:59:59.000Z

191

Quantitative Influences of Butyrate or Propionate on Thermophilic Production of Methane from Biomass  

Science Journals Connector (OSTI)

...Gainesville, Florida 32611 * Corresponding...address: Solar Energy Research Institute...Golden, CO 80401. Florida Agricultural Experiment...8. Infusion rates were started at...Corresponding author. t Florida Agricultural Experiment...address: Solar Energy Research Institute...increasing the apparent rate of formation of...

J. Michael Henson; F. M. Bordeaux; Christopher J. Rivard; P. H. Smith

1986-02-01T23:59:59.000Z

192

Ethanol production by thermophilic bacteria: metabolic control of end product formation in Thermoanaerobium brockii.  

Science Journals Connector (OSTI)

...monoculture, T. brockii grew on ethanol as the energy source, and acetate and methane...monoculture, T. brockii grew on ethanol as the energy source, and acetate and methane...monoculture, T. brockii grew on ethanol as the energy source, and acetate and methane...

A Ben-Bassat; R Lamed; J G Zeikus

1981-04-01T23:59:59.000Z

193

Anaerobic Digestion of Renewable Biomass: Thermophilic Temperature Governs Methanogen Population Dynamics  

Science Journals Connector (OSTI)

...maize from a biogas plant in Nordhausen, Germany), 1/3 sewage sludge (from a wastewater treatment plant [WWTP] in Geesthacht, Germany), and 1/3 hot-rot compost suspension (compost from grass and garden greens, without solids). After a couple...

Niclas Krakat; A. Westphal; S. Schmidt; P. Scherer

2010-01-22T23:59:59.000Z

194

A Moderately Thermophilic Mixed Microbial Culture for Bioleaching of Chalcopyrite Concentrate at High Pulp Density  

Science Journals Connector (OSTI)

...chalcopyrite) served as energy sources. After that...about 70% of copper reserves in the world (1). It is also the...chalcopyrite, and high lattice energy (1, 3, 4). Bioleaching...bioleaching of chalcopyrite. World J. Microbiol. Biotechnol...

Yuguang Wang; Weimin Zeng; Guanzhou Qiu; Xinhua Chen; Hongbo Zhou

2013-11-15T23:59:59.000Z

195

Isolation of plasmids present in thermophilic strains from hot springs in Jordan Amjad B. Khalil1,  

E-Print Network [OSTI]

in shaker and agar plate culture in Thermus medium (ATCC 697). Isolation of plasmid DNA-small scale to possess a single site for both plasmids, this enzyme is EcoRI. Introduction Plasmid purification Thermotoga strain (Akimkina et al. 1999). This report describes the development of rapid, reproducible, small

Khalil, Amjad

196

Thermophilic and thermoacidophilic sugar transporter genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods  

DOE Patents [OSTI]

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for transporting sugars across cell membranes using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Reed, David W.; Lacey, Jeffrey A.

2013-01-15T23:59:59.000Z

197

Thermophilic and thermoacidophilic sugar transporter genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods  

DOE Patents [OSTI]

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for transporting sugars across cell membranes using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Thompson, David N.; Apel, William A.; Thompson, Vicki S.; Reed, David W.; Lacey, Jeffrey A.

2013-01-29T23:59:59.000Z

198

Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme  

Science Journals Connector (OSTI)

...fuel sources and most sustainable energy resource and is reproduced...also financed by the New Energy and Industrial Technology...from lignocellulose: a challenge for metabolic engineering and process integration. Appl. Microbiol...

Yasuya Fujita; Junji Ito; Mitsuyoshi Ueda; Hideki Fukuda; Akihiko Kondo

2004-02-01T23:59:59.000Z

199

Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways  

SciTech Connect (OSTI)

Clostridium thermocellum is a thermophilic, cellulolytic anaerobe that is a candidate microorganism for industrial biofuels production. Strains with mutations in genes associated with production of L-lactate (Dldh) and/or acetate (Dpta) were characterized to gain insight into the intracellular processes that convert cellobiose to ethanol and other fermentation end-products. Cellobiose-grown cultures of the Dldh strain had identical biomass accumulation, fermentation end-products, transcription profile, and intracellular metabolite concentrations compared to its parent strain (DSM1313 Dhpt Dspo0A). The Dpta-deficient strain grew slower and had 30 % lower final biomass concentration compared to the parent strain, yet produced 75% more ethanol. A Dldh Dpta double-mutant strain evolved for faster growth had a growth rate and ethanol yield comparable to the parent strain, whereas its biomass accumulation was comparable to Dpta. Free amino acids were secreted by all examined strains, with both Dpta strains secreting higher amounts of alanine, valine, isoleucine, proline, glutamine, and threonine. Valine concentration for Dldh Dpta reached 5 mM by the end of growth, or 2.7 % of the substrate carbon utilized. These secreted amino acid concentrations correlate with increased intracellular pyruvate concentrations, up to sixfold in the Dpta and 16-fold in the Dldh Dpta strain. We hypothesize that the deletions in fermentation end-product pathways result in an intracellular redox imbalance, which the organism attempts to relieve, in part by recycling NADP* through increased production of amino acids.

Van Der Veen, Douwe [ORNL; Lo, Jonathan [Dartmouth College; Brown, Steven D [ORNL; Johnson, Courtney M [ORNL; Tschaplinski, Timothy J [ORNL; Martin, Madhavi Z [ORNL; Engle, Nancy L [ORNL; Van den Berg, Robert A [Katholieke University Leuven, Belgium; Argyros, Aaron [Mascoma Corporation; Caiazza, Nicky [Mascoma Corporation; Guss, Adam M [ORNL; Lynd, Lee R [Thayer School of Engineering at Dartmouth

2013-01-01T23:59:59.000Z

200

Characterization of Clostridium thermocellum strains with disrupted fermentation end product pathways  

SciTech Connect (OSTI)

Clostridium thermocellum is a thermophilic, cellulolytic anaerobe that is a candidate microorganism for industrial biofuels production. Strains with mutations in genes associated with production of Llactate ( ldh) and/or acetate ( pta) were characterized to gain insight into the intracellular processes that convert cellobiose to ethanol and other fermentation end products. Cellobiose-grown cultures of the ldh strain had identical biomass accumulation, fermentation end products, transcription profile and intracellular metabolite concentrations compared to its parent strain (DSM1313 hpt spo0A). The pta-deficient strain grew slower and had 30% lower final biomass concentration compared to the parent strain, yet produced 75% more ethanol. A ldh pta double mutant strain evolved for faster growth had growth rate and ethanol yield comparable to the parent strain, whereas its biomass accumulation was comparable to pta. Free amino acids were secreted by all examined strains, with both pta strains secreting higher amounts of alanine, valine, isoleucine, proline, glutamine, and threonine. Valine concentration for ldh pta reached 5 mM by the end of growth, or 2.7% of the substrate carbon utilized. These secreted amino acid concentrations correlate with increased intracellular pyruvate concentrations, up to 6-fold in the pta and 16-fold in the ldh pta strain. We hypothesize that the deletions in fermentation end product pathways result in an intracellular redox imbalance, which the organism attempts to relieve, in part by recycling NADP+ through increased production of amino acids.

Van Der Veen, Douwe [ORNL; Lo, Jonathan [Dartmouth College; Brown, Steven D [ORNL; Johnson, Courtney M [ORNL; Tschaplinski, Timothy J [ORNL; Martin, Madhavi Z [ORNL; Engle, Nancy L [ORNL; Argyros, Aaron [Mascoma Corporation; Van den Berg, Robert A [Katholieke University Leuven, Belgium; Caiazza, Nicky [Mascoma Corporation; Guss, Adam M [ORNL; Lynd, Lee R [Thayer School of Engineering at Dartmouth

2013-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

6099.pdf  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Nov. Nov. 2010, p. 6099-6100 Vol. 192, No. 22 0021-9193/10/$12.00 doi:10.1128/JB.00950-10 Copyright © 2010, American Society for Microbiology. All Rights Reserved. GENOME ANNOUNCEMENTS Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47 Tᰔ James G. Elkins, 1,2 * Adriane Lochner, 1,2 Scott D. Hamilton-Brehm, 1,2 Karen Walston Davenport, 3 Mircea Podar, 1,2 Steven D. Brown, 1,2 Miriam L. Land, 2 Loren J. Hauser, 1,2 Dawn M. Klingeman, 1,2 Babu Raman, 1,2 Lynne A. Goodwin, 3 Roxanne Tapia, 3 Linda J. Meincke, 3 J. Chris Detter, 4 David C. Bruce, 3 Cliff S. Han, 3 Anthony V. Palumbo, 1,2 Robert W. Cottingham, 1,2 Martin Keller, 1,2 and David E. Graham 2 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 1 ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 2 ; Los Alamos National

202

Extended abstract Fibre degrading enzymes, their origin and diversity  

E-Print Network [OSTI]

of this species is considered to be xylan degradation. Other secondary cellulolytic species (found sporadically- tienzyme complexes at the surface or in the extracellular culture medium of the main cellulolytic bacterial

Boyer, Edmond

203

NREL: Energy Sciences - Kara Podkaminer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Kara Podkaminer Kara Podkaminer Postdoctoral Researcher Photo of Kara Podkaminer Phone: (303) 384-7970 Email: Kara.Podkaminer@nrel.gov At NREL Since: 2011 Kara Podkaminer received her Ph.D. in Engineering Sciences from the Thayer School of Engineering at Dartmouth College in 2011. For her dissertation work, she studied the thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum ALK2 and its application in a thermophliic SSF process. At NREL, Dr. Podkaminer is working on heterologous protein expression in T. reesei, looking to better understand the bottlenecks and increase protein production. This work will serve as the foundation for future expression of NREL's chimera proteins. Printable Version NREL is a national laboratory of the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, operated by the Alliance for Sustainable Energy, LLC.

204

acs_PR_pr-2011-00536j 1..13  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

1, 1, 2011 r 2011 American Chemical Society 5302 dx.doi.org/10.1021/pr200536j | J. Proteome Res. 2011, 10, 5302-5314 ARTICLE pubs.acs.org/jpr Label-free Quantitative Proteomics for the Extremely Thermophilic Bacterium Caldicellulosiruptor obsidiansis Reveal Distinct Abundance Patterns upon Growth on Cellobiose, Crystalline Cellulose, and Switchgrass Adriane Lochner, †,‡,§,|| Richard J. Giannone, ‡,||,^ Martin Keller, †,‡ Garabed Antranikian, § David E. Graham,* ,†,# and Robert L. Hettich* ,‡,^ † Biosciences Division; ^ Chemical Sciences Division; ‡ BioEnergy Science Center at Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, United States § Technical Microbiology, Hamburg University of Technology, Kasernenstrasse 12, D-21073 Hamburg, Germany # Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996,

205

Why Sequence Bacillus coagulans?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Bacillus coagulans? Bacillus coagulans? Bacilus coagulans strain 36D1 and its close relatives are ideal biocatalysts for fermentation of lignocellulosic biomass to fuels and chemicals. This bacterium is an acidophile and a moderate thermophile (grows at pH 5.0 and at 55°C). These characteristics are similar to the optimal conditions for the activity of fungal cellulases, which have been developed with significant support from DOE for simultaneous saccharification and fermentation (SSF) of cellulose to useful products (fuel ethanol, lactic acid, etc.). The optimal growth conditions for the biocatalysts currently used by industry for production of ethanol or lactic acid significantly differ from the optimal conditions for cellulase activity. This mismatch leads to higher usage of expensive cellulases than

206

Complete genome sequence of Thermaerobacter marianensis type strain (7p75aT)  

SciTech Connect (OSTI)

Thermaerobacter marianensis Takai et al. 1999 is the type species of the genus Thermaerobacter, which belongs to the Clostridiales family Incertae Sedis XVII. The species is of special interest because T. marianensis is an aerobic, thermophilic marine bacterium, originally isolated from the deepest part in the western Pacific Ocean (Mariana Trench) at the depth of 10.897m. Interestingly, the taxonomic status of the genus has not been clarified until now. The genus Thermaerobacter may represent a very deep group within the Firmicutes or potentially a novel phylum. The 2,844,696 bp long genome with its 2,375 protein-coding and 60 RNA genes consists of one circular chromosome and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Han, Cliff [Los Alamos National Laboratory (LANL); Gu, Wei [U.S. Department of Energy, Joint Genome Institute; Hackett, M. [University of Washington; Zhang, Xiaojing [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Chang, Yun-Juan [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Schneider, Susan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Pukall, Rudiger [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute

2010-01-01T23:59:59.000Z

207

Environmental genomics reveals a single species ecosystem deep within the Earth  

SciTech Connect (OSTI)

DNA from low biodiversity fracture water collected at 2.8 km depth in a South African gold mine was sequenced and assembled into a single, complete genome. This bacterium, Candidatus Desulforudis audaxviator, comprises>99.9percent of the microorganisms inhabiting the fluid phase of this particular fracture. Its genome indicates a motile, sporulating, sulfate reducing, chemoautotrophic thermophile that can fix its own nitrogen and carbon using machinery shared with archaea. Candidatus Desulforudis audaxviator is capable of an independent lifestyle well suited to long-term isolation from the photosphere deep within Earth?s crust, and offers the first example of a natural ecosystem that appears to have its biological component entirely encoded within a single genome.

Chivian, Dylan; Brodie, Eoin L.; Alm, Eric J.; Culley, David E.; Dehal, Paramvir S.; DeSantis, Todd Z.; Gihring, Thomas M.; Lapidus, Alla; Lin, Li-Hung; Lowry, Stephen R.; Moser, Duane P.; Richardson, Paul; Southam, Gordon; Wanger, Greg; Pratt, Lisa M.; Andersen, Gary L.; Hazen, Terry C.; Brockman, Fred J.; Arkin, Adam P.; Onstott, Tullis C.

2008-09-17T23:59:59.000Z

208

Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge  

Science Journals Connector (OSTI)

...compounds (23). According to the literature, hydrogenotrophic methanogenesis...conversion of biomass to methane: a review. Rev. Environ. Sci. Biotechnol...carbon isotopic signatures: a review and a proposal. Org. Geochem...minimisation and biomaterials/bioenergy recovery technologies. John...

Dang P. Ho; Paul D. Jensen; Damien J. Batstone

2013-08-16T23:59:59.000Z

209

Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge  

Science Journals Connector (OSTI)

...biomass to methane: a review. Rev. Environ. Sci...isotopic signatures: a review and a proposal. Org...marine and freshwater environments: CO2 reduction vs...microbes in methanogenic environments. Microbes Environ...minimisation and biomaterials/bioenergy recovery technologies...

Dang P. Ho; Paul D. Jensen; Damien J. Batstone

2013-08-16T23:59:59.000Z

210

Genome Sequences of Two Thermophilic Bacillus licheniformis Strains, Efficient Producers of Platform Chemical 2,3-Butanediol  

Science Journals Connector (OSTI)

...Efficient Producers of Platform Chemical 2,3-Butanediol...3-BD is a crucial platform compound, which could...antibiotics, and chemicals and have been identified...efficient producers of platform chemical 2,3-butanediol...

Lixiang Li; Fei Su; Yu Wang; Lijie Zhang; Cuicui Liu; Jingwen Li; Cuiqing Ma; Ping Xu

2012-08-01T23:59:59.000Z

211

A newly isolated Bacillus licheniformis strain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical  

Science Journals Connector (OSTI)

...2,3-Butanediol (2,3-BD), a platform and fuel bio-chemical, can be efficiently produced by Klebsiella pneumonia, K. oxytoca, and Serratia marcescens. However, these strains are opportunistic pathogens and not f...

Lixiang Li; Lijie Zhang; Kun Li; Yu Wang; Chao Gao…

2013-08-01T23:59:59.000Z

212

Complete genome of the cellyloytic thermophile Acidothermus cellulolyticus 11B provides insights into its ecophysiological and evloutionary adaptations  

E-Print Network [OSTI]

functions (Table 2). The GH13 alpha amylase (Acel_0679) mayenzyme Trehalose synthase Alpha amylase Glycogen debranching

Barabote, Ravi D.

2009-01-01T23:59:59.000Z

213

Analysis of Metabolic Pathways and Fluxes in a Newly Discovered Thermophilic and Ethanol-Tolerant Geobacillus Strain  

E-Print Network [OSTI]

Bacteria engineered for fuel ethanol production: currentcharacterization of two novel ethanol-tolerant facultative-Lin Y, Tanaka S. 2006. Ethanol fermentation from biomass

Tang, Yinjie J.

2009-01-01T23:59:59.000Z

214

Complete genome sequence of the moderately thermophilic mineral-sulfide-oxidizing firmicute Sulfobacillus acidophilus type strain (NALT)  

SciTech Connect (OSTI)

Sulfobacillus acidophilus Norris et al. 1996 is a member of the genus Sulfobacillus which comprises five species of the order Clostridiales. Sulfobacillus species are of interest for comparison to other sulfur and iron oxidizers and also have biomining applications. This is the first completed genome sequence of a type strain of the genus Sulfobacillus, and the second published genome of a member of the species S. acidophilus. The genome, which consists of one chromosome and one plasmid with a total size of 3,557,831 bp, harbors 3,626 protein-coding and 69 RNA genes, and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Anderson, Iain [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Saunders, Elizabeth H [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Pan, Chongle [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Pukall, Rudiger [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

215

Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes  

DOE Patents [OSTI]

A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

Thompson, David N; Apel, William A; Thompson, Vicki S; Ward, Thomas E

2014-04-08T23:59:59.000Z

216

Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes  

DOE Patents [OSTI]

A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

Thompson, David N; Apel, William A; Thompson, Vicki S; Ward, Thomas E

2013-07-23T23:59:59.000Z

217

The Genetically Remote Pathogenic Strain NVH391-98 of the Bacillus cereus Group Represents the Cluster of Thermophilic Strains  

E-Print Network [OSTI]

and from a B. cereus food-poisoning strain. Microbiology.are known to cause food poisoning. A rare phylogeneticallyK presumably responsible for food poisoning. This pathogenic

Auger, Sandrine

2008-01-01T23:59:59.000Z

218

Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid  

Science Journals Connector (OSTI)

...and the State of Florida, University of Florida Agricultural Experiment...National Renewable Energy Laboratory, Golden...waste cellulosics to fuel ethanol: a review...on transportation fuels. Annu. Rev. Energy Environ. 25: 199-244...

Milind A. Patel; Mark S. Ou; Roberta Harbrucker; Henry C. Aldrich; Marian L. Buszko; Lonnie O. Ingram; K. T. Shanmugam

2006-05-01T23:59:59.000Z

219

Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid  

Science Journals Connector (OSTI)

...TP-510-32438. National Renewable Energy Laboratory, Golden, Colo...potential to reduce the cost of SSF by minimizing the...cellulases, a significant cost component in the use of biomass as a renewable resource, for the production...

Milind A. Patel; Mark S. Ou; Roberta Harbrucker; Henry C. Aldrich; Marian L. Buszko; Lonnie O. Ingram; K. T. Shanmugam

2006-05-01T23:59:59.000Z

220

E-Print Network 3.0 - antagonistic bacterium belonging Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collection: Biology and Medicine ; Environmental Sciences and Ecology 13 Heavy Metal Tolerance in Stenotrophomonas maltophilia Delphine Pages1,2,3 Summary:...

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Biodegradation of triclosan by a triclosan-degrading isolate and an ammonia-oxidizing bacterium.  

E-Print Network [OSTI]

??Triclosan is incorporated in a wide array of medical and consumer products as an antimicrobial agent or preservative. Disposal of these products transport triclosan into… (more)

Zhao, Fuman

2007-01-01T23:59:59.000Z

222

Reconstitution of a Thermostable Xylan-Degrading Enzyme Mixture from the Bacterium Caldicellulosiruptor bescii  

Science Journals Connector (OSTI)

...been described in the literature. Our group has also...depolymerization of xylans in bioenergy feedstock at high temperatures...Glob. Change Biol. Bioenergy 1 :2-17. 9. Kumar...and F Cherchi. 2012. Review of pretreatment processes...innovative method. Biomass Bioenergy 46 :25-35. 11...

Xiaoyun Su; Yejun Han; Dylan Dodd; Young Hwan Moon; Shosuke Yoshida; Roderick I. Mackie; Isaac K. O. Cann

2012-12-21T23:59:59.000Z

223

Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae  

Science Journals Connector (OSTI)

...costs and benefits of biodiesel and ethanol biofuels...Life-cycle assessment of biodiesel production from microalgae...symbiosis involving a green alga (Chlamydomonas), a...aquatic species program: biodiesel from algae. U.S. Department...

Juan Cesar Federico Ortiz-Marquez; Mauro Do Nascimento; Maria de los Angeles Dublan; Leonardo Curatti

2012-01-20T23:59:59.000Z

224

Rapid Aggregation of Biofuel-Producing Algae by the Bacterium Bacillus sp. Strain RP1137  

Science Journals Connector (OSTI)

...switchgrass. Fuels from algae are often listed as the...other biofuels. The term alga-derived biofuels refers to the production of biodiesel from algal lipids, as...second-generation approaches. Biodiesel can also be generated...among the advantages of algae is the productivity that...

Ryan J. Powell; Russell T. Hill

2013-07-26T23:59:59.000Z

225

Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae  

Science Journals Connector (OSTI)

...strategy for the mitigation of the greenhouse...share of the total energy inputs in agriculture...significant input of energy if wastewater is...fuel oil use by renewable sources, even accounting...of all the fossil energy input for large-scale...concerns related to the mitigation of global warming...

Juan Cesar Federico Ortiz-Marquez; Mauro Do Nascimento; Maria de los Angeles Dublan; Leonardo Curatti

2012-01-20T23:59:59.000Z

226

Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation  

Science Journals Connector (OSTI)

...Phylogenetically, ND132 falls into a large group of salt-tolerant...Mercury Science Focus Area Program at Oak Ridge National...Minnesota): importance of atmospheric deposition and ecosystem...Article Research Support, Non-U.S. Gov't Research...Support, U.S. Gov't, Non-P.H.S. | 0 DNA...

Cynthia C. Gilmour; Dwayne A. Elias; Amy M. Kucken; Steven D. Brown; Anthony V. Palumbo; Christopher W. Schadt; Judy D. Wall

2011-04-22T23:59:59.000Z

227

Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae  

Science Journals Connector (OSTI)

...Biofuel alternatives to ethanol...microalgae on the biofuels priority...in solar energy conversion...involving a green alga (Chlamydomonas...Department of Energy's aquatic...biodiesel from algae. U.S. Department of Energy, Golden...Carbon-negative biofuels from low-input...and other alternative sources of...

Juan Cesar Federico Ortiz-Marquez; Mauro Do Nascimento; Maria de los Angeles Dublan; Leonardo Curatti

2012-01-20T23:59:59.000Z

228

E-Print Network 3.0 - antarctic bacterium pseudoalteromonas Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Microbiology and Blackwell Publishing Ltd Summary: % sequence similarity with the CFB group. The main genera of gggg-Proteobacteria were Pseudoalteromonas (14... Vibrio and...

229

Structural characteristics of alkaline phosphatase from the moderately halophilic bacterium Halomonas sp. 593  

Science Journals Connector (OSTI)

In order to clarify the structural basis of the halophilic characteristics of an alkaline phosphatase derived from the moderate halophile Halomonas sp. 593 (HaAP), the tertiary structure of HaAP was determined to 2.1 ? resolution by X-ray crystallography. The structural properties of surface negative charge and core hydrophobicity were shown to be intermediate between those characteristic of halophiles and non-halophiles, and may explain the unique functional adaptation to a wide range of salt concentrations.

Arai, S.

2014-02-22T23:59:59.000Z

230

Genome sequence of the soil bacterium Saccharomonospora azurea type strain (NA-128T)  

E-Print Network [OSTI]

Lucas S, Chen F, Tice H, Pitluck S, et al. Complete genome3 , Hope Tice 3 , Sam Pitluck 3 , David Bruce 2,3 , Lynne

Klenk, Hans-Peter

2014-01-01T23:59:59.000Z

231

Genome sequence of the ocean sediment bacterium Saccharomonospora marina type strain (XMU15T)  

E-Print Network [OSTI]

Lucas S, Chen F, Tice H, Pitluck S, et al. Complete genome3 , Alex Copeland 3 , Sam Pitluck 3 , Lynne Goodwin 2,3 ,

Klenk, Hans-Peter

2014-01-01T23:59:59.000Z

232

Complete Genome Sequence of the Haloalkaliphilic, Hydrogen-Producing Bacterium Halanaerobium hydrogeniformans  

Science Journals Connector (OSTI)

...Steven D. Brown 1 Matthew B. Begemann 2 Melanie R. Mormile 3 Judy D. Wall 4 Cliff S. Han 5 6 Lynne A. Goodwin 5 6 Samuel Pitluck 6 7 Miriam L. Land 1 6 Loren J. Hauser 1 6 Dwayne A. Elias 1 * * Corresponding author. Mailing address: Biosciences Division...

Steven D. Brown; Matthew B. Begemann; Melanie R. Mormile; Judy D. Wall; Cliff S. Han; Lynne A. Goodwin; Samuel Pitluck; Miriam L. Land; Loren J. Hauser; Dwayne A. Elias

2011-05-20T23:59:59.000Z

233

Complete genome sequence of the melanogenic marine bacterium Marinomonas mediterranea type strain (MMB-1T)  

SciTech Connect (OSTI)

Marinomonas mediterranea MMB-1 T Solano & Sanchez-Amat 1999 belongs to the family Oceanospirillaceae within the phylum Proteobacteria. This species is of interest because it is the only species described in the genus Marinomonas to date that can synthesize melanin pigments, which is mediated by the activity of a tyrosinase. M. mediterranea expresses other oxidases of biotechnological interest, such as a multicopper oxidase with laccase activity and a novel L-lysine-epsilon-oxidase. The 4,684,316 bp long genome harbors 4,228 proteincoding genes and 98 RNA genes and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Lucas-Elio, Patricia [University of Murcia, Murcia, Spain; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Detter, J C [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Johnston, Andrew W. B. [University of East Anglia, Norwich, United Kingdom; Sanchez-Amat, Antonio [University of Murcia, Murcia, Spain

2012-01-01T23:59:59.000Z

234

Genome Sequence of Chthoniobacter flavus Ellin428, an aerobic heterotrophic soil bacterium  

SciTech Connect (OSTI)

Chthoniobacter flavusis Ellin428 is the first isolate from subdivision 2 of the bacterial phylum Verrucomicrobia. C. flavusis Ellin428 can metabolize many of the saccharide components of plant biomass but does not grow with amino acids or organic acids other than pyruvate.

Kant, Ravi [University of Helsinki; Van Passel, Mark W.J. [Wageningen University and Research Centre, The Netherlands; Palva, Airi [University of Helsinki; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Larimer, Frank W [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; De Vos, Willem M. [Wageningen University and Research Centre, The Netherlands; Janssen, Peter H. [AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand; Smidt, Hauke [Wageningen University and Research Centre, The Netherlands

2011-01-01T23:59:59.000Z

235

Complete genome sequence of the facultatively anaerobic, appendaged bacterium Muricauda ruestringensis type strain (B1T)  

SciTech Connect (OSTI)

Muricauda ruestringensis Bruns et al. 2001 is the type species of the genus Muricauda, which belongs to the family Flavobacteriaceae in the phylum Bacteroidetes. The species is of interest because of its isolated position in the genomically unexplored genus Muricauda, which is located in a part of the tree of life containing not many organisms with sequenced genomes. The genome, which consists of a circular chromosome of 3,842,422 bp length with a total of 3,478 protein-coding and 47 RNA genes, is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Pan, Chongle [ORNL; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

236

Genome Sequence of Chthoniobacter flavus Ellin428, an Aerobic Heterotrophic Soil Bacterium  

Science Journals Connector (OSTI)

...Susan Lucas 3 4 Alla Lapidus 3 5 Tijana Glavina del Rio 3 5 Eileen Dalin 3 5 Hope Tice 3 5 David Bruce 6 Lynne Goodwin 6 Sam Pitluck 5 Frank W. Larimer 7 Miriam L. Land 7 Loren Hauser 7 Parveen Sangwan 8 Willem M. de Vos 1 2 Peter H. Janssen 9 Hauke Smidt...

Ravi Kant; Mark W. J. van Passel; Airi Palva; Susan Lucas; Alla Lapidus; Tijana Glavina del Rio; Eileen Dalin; Hope Tice; David Bruce; Lynne Goodwin; Sam Pitluck; Frank W. Larimer; Miriam L. Land; Loren Hauser; Parveen Sangwan; Willem M. de Vos; Peter H. Janssen; Hauke Smidt

2011-04-01T23:59:59.000Z

237

Predicting and Manipulating Cardiac Drug Inactivation by the Human Gut Bacterium Eggerthella lenta  

Science Journals Connector (OSTI)

...B. Lapidus A. Glavina Del Rio T. Copeland A. Tice H. Cheng J. F. Lucas S. Chen F. Nolan M. Bruce D. Goodwin L. Pitluck S. Ivanova N. Mavromatis K. Ovchinnikova G. Pati A. Chen A. Palaniappan K. Land M. Hauser L. Chang Y. J. Jeffries...

Henry J. Haiser; David B. Gootenberg; Kelly Chatman; Gopal Sirasani; Emily P. Balskus; Peter J. Turnbaugh

2013-07-19T23:59:59.000Z

238

Genome sequence of the chemoheterotrophic soil bacterium Saccharomonospora cyanea type strain (NA-134(T))  

SciTech Connect (OSTI)

Saccharomonospora cyanea Runmao et al. 1988 is a member of the genus Saccharomonospora in the family Pseudonocardiaceae that is moderately well characterized at the genome level thus far. Members of the genus Saccharomonospora are of interest because they originate from diverse habitats, such as soil, leaf litter, manure, compost, surface of peat, moist, over-heated grain, and ocean sediment, where they probably play a role in the primary degradation of plant material by attacking hemicellulose. Species of the genus Saccharomonospora are usually Gram-positive, non-acid fast, and are classified among the actinomycetes. S. cyanea is characterized by a dark blue (= cyan blue) aerial mycelium. After S. viridis, S. azurea, and S. marina, S. cyanea is only the fourth member in the genus for which a completely sequenced (non-contiguous finished draft status) type strain genome will be published. Here we describe the features of this organism, together with the draft genome sequence, and annotation. The 5,408,301 bp long chromosome with its 5,139 protein-coding and 57 RNA genes was sequenced as part of the DOE funded Community Sequencing Program (CSP) 2010 at the Joint Genome Institute (JGI).

Meier-Kolthoff, Jan P. [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany] [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lu, Megan [Los Alamos National Laboratory (LANL)] [Los Alamos National Laboratory (LANL); Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL)] [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL)] [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL)] [Los Alamos National Laboratory (LANL); Potter, Gabriele [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany] [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Land, Miriam L [ORNL] [ORNL; Ivanova, N [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany] [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany] [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute] [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany] [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2013-01-01T23:59:59.000Z

239

Complete genome sequence of the aquatic bacterium Runella slithyformis type strain (LSU 4T)  

SciTech Connect (OSTI)

Runella slithyformis Larkin and Williams 1978 is the type species of the genus Runella, which belongs to the family Cytophagaceae. The species is of interest because it is able to grow at temperatures as low as 4 C. This is the first completed genome sequence of a member of the genus Runella and the sixth sequence from the family Cytophagaceae. The 6,919,729 bp long genome consists of a 6.6 Mbp circular genome and five circular plasmids of 38.8 to 107.0 kbp length, harboring a total of 5,974 protein-coding and 51 RNA genes and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Copeland, A [U.S. Department of Energy, Joint Genome Institute; Zhang, Xiaojing [Los Alamos National Laboratory (LANL); Misra, Monica [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Pan, Chongle [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Detter, J C [U.S. Department of Energy, Joint Genome Institute; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Ngatchou, Olivier Duplex [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Sikorski, Johannes [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Tindall, Brian [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

240

Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae  

Science Journals Connector (OSTI)

...FIBA, Mar del Plata, Argentina, and Centro de Estudios...CONICET, Mar del Plata, Argentina Concerns regarding the...alternative sources of energy during the last years...result of the fossil energy inputs needed to capture...ponds in Buenos Aires, Argentina, in 2009 and were identified...

Juan Cesar Federico Ortiz-Marquez; Mauro Do Nascimento; Maria de los Angeles Dublan; Leonardo Curatti

2012-01-20T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

A siderophore from a marine bacterium with an exceptional ferric ion affinity constant  

Science Journals Connector (OSTI)

... conformation of alterobactin A and the iron(m)-altero-bactin A complex using HyperChem (Autodesk, Inc). The predicted conformation of alterobactin A (a) was obtained by minimization ...

Richard T. Reid; David H. Livet; D. John Faulkner; Alison Butler

1993-12-02T23:59:59.000Z

242

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus  

Science Journals Connector (OSTI)

...Individual images were labeled with Adobe Illustrator CS5 (Adobe Systems). Animations were generated by using Autodesk Maya 2011 (Autodesk). 1 Schmidt JM Stanier RY ( 1965 ) Isolation and characterization of bacteriophages active against stalked...

Ricardo C. Guerrero-Ferreira; Patrick H. Viollier; Bert Ely; Jeanne S. Poindexter; Maria Georgieva; Grant J. Jensen; Elizabeth R. Wright

2011-01-01T23:59:59.000Z

243

E-Print Network 3.0 - acidophilic heterotrophic bacterium Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

suillum did not grow heterotrophically or lithoautotrophically... capable of Fe(II) oxidation. In the aerobic acidophilic Fe(II) oxidizer Thiobacillus ferrooxi- dans... )...

244

Reconstitution of a Thermostable Xylan-Degrading Enzyme Mixture from the Bacterium Caldicellulosiruptor bescii  

Science Journals Connector (OSTI)

...Reconstitution of a Thermostable Xylan-Degrading Enzyme Mixture from...Xylose, the major constituent of xylans, as well as the side chain...Sun and H Zhao. 2010. Yeast surface display of trifunctional minicellulosomes...Enzymatic deconstruction of xylan for biofuel production. Glob...

Xiaoyun Su; Yejun Han; Dylan Dodd; Young Hwan Moon; Shosuke Yoshida; Roderick I. Mackie; Isaac K. O. Cann

2012-12-21T23:59:59.000Z

245

Bioluminescence in a strain of the human pathogenic bacterium Vibrio vulnificus.  

Science Journals Connector (OSTI)

...soft-tissue infections caused by marine vibrios. Surgery 98:126-130...and other lactose- fermenting marine vibrios in coastal waters of...lactose-fermenting vibrios in the marine environment. Appl. Environ...Oliver, J. D., J. E. Wear, M. B. Thomas, M. Warner...

J D Oliver; D M Roberts; V K White; M A Dry; L M Simpson

1986-11-01T23:59:59.000Z

246

Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia  

Science Journals Connector (OSTI)

... primarily through the microbial degradation of organic matter, but abiogenic methane emitted from seeps and geothermal areas is also a major contribution to the atmospheric budget, estimated at 45–75? ... at 45–75?Tg annually. Hell’s Gate (Tikitere), New Zealand, is a geothermal area rich in abiogenic methane. We studied methane oxidation in an area of woody ...

Peter F. Dunfield; Anton Yuryev; Pavel Senin; Angela V. Smirnova; Matthew B. Stott; Shaobin Hou; Binh Ly; Jimmy H. Saw; Zhemin Zhou; Yan Ren; Jianmei Wang; Bruce W. Mountain; Michelle A. Crowe; Tina M. Weatherby; Paul L. E. Bodelier; Werner Liesack; Lu Feng; Lei Wang; Maqsudul Alam

2007-11-14T23:59:59.000Z

247

Polyphosphate Storage during Sporulation in the Gram-Negative Bacterium Acetonema longum  

Science Journals Connector (OSTI)

...111-129. 30. Parsons AJ , I Ahmed, CD Rudd, GJ Cuello, E Pellegrini, D Richard, and MR Johnson. 2010. Neutron scattering and ab initio molecular dynamics study of cross-linking in biomedical phosphate glasses. J. Phys. Condens...

Elitza I. Tocheva; Anne E. Dekas; Shawn E. McGlynn; Dylan Morris; Victoria J. Orphan; Grant J. Jensen

2013-06-28T23:59:59.000Z

248

Coaggregation by the Freshwater Bacterium Sphingomonas natatoria Alters Dual-Species Biofilm Formation  

Science Journals Connector (OSTI)

...has been isolated from swimming pools, deep-ice boreholes...source of carbon and energy. Another study that...occurs with enhanced efficiency in biofilms and induces...bacteria isolated from a swimming pool. J. Appl. Bacteriol...

K. R. Min; A. H. Rickard

2009-04-17T23:59:59.000Z

249

Novel Electrochemically Active Bacterium Phylogenetically Related to Arcobacter butzleri, Isolated from a Microbial Fuel Cell  

Science Journals Connector (OSTI)

...FJ968636 to FJ968639. RESULTS Acetate consumption and generation of potential in the MFC...were taken and used to measure acetate consumption in flasks with a carbon anode and in...community in the MFC (Fig. 1). This consumption of substrate was accompanied by a decrease...

Viatcheslav Fedorovich; Matthew C. Knighton; Eulyn Pagaling; F. Bruce Ward; Andrew Free; Igor Goryanin

2009-10-02T23:59:59.000Z

250

An insect symbiosis is influenced by bacterium-specific polymorphisms in  

E-Print Network [OSTI]

with Escherichia coli K12 were lethal. In contrast, tsetse were resistant to an E. coli OmpA mutant strain, whereas recombinant Sodalis expressing E. coli OmpA became pathogenic. Profiling of tsetse immunity-related gene infection with otherwise lethal E. coli K12. Our results show that polymorphisms in the exposed loop domains

Aksoy, Serap

251

Genome sequence of the ocean sediment bacterium Saccharomonospora marina type strain (XMU15T)  

SciTech Connect (OSTI)

Saccharomonospora marina Liu et al. 2010 is a member to the genomically so far poorly characterized genus Saccharomonospora in the family Pseudonocardiaceae. Members of the genus Sacharomonospora are of interest because they originate from diverse habitats, such as leaf litter, manure, compost, surface of peat, moist, over-heated grain, and ocean sediment, where they might play a role in the primary degradation of plant material by attacking hemicellulose. Organisms belonging to the genus are usually Gram-positive staining, non-acid fast, and classify among the actinomycetes. Next to S. viridis and S. azurea, S. marina is the third member in the genus Saccharomonospora for with a completely sequenced (permanent draft status) type strain genome will be published. Here we describe the features of this organism, together with the complete genome sequence, and annotation. The 5,965,593 bp long chromosome with its 5,727 protein-coding and 57 RNA genes was sequenced as part of the DOE funded Community Sequencing Program (CSP) 2010 at the Joint Genome Institute (JGI).

Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lu, Megan [Los Alamos National Laboratory (LANL); Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Potter, Gabriele [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Land, Miriam L [ORNL; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Li, Wen-Jun [Yunnan University, Kunming, China; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

252

Genome sequence of the soil bacterium Saccharomonospora azurea type strain (NA-128T)  

SciTech Connect (OSTI)

Saccharomonospora azurea Runmao et al. 1987 is a member to the genomically so far poorly characterized genus Saccharomonospora in the family Pseudonocardiaceae. Members of the genus Sacharomonosoras are of interest because they originate from diverse habitats, such as leaf litter, manure, compost, surface of peat, moist and over-heated grain, where they might play a role in the primary degradation of plant material by attacking hemicellulose. They are Gram-negative staining organisms classified among the usually Gram-positive actinomycetes. Next to S. viridis, S. azurea is only the second member in the genus Saccharomonospora for with a completely sequenced type strain genome will be published. Here we describe the features of this organism, together with the complete genome sequence with project status 'permanent draft', and annotation. The 4,763,832 bp long chromosome with its 4,472 protein-coding and 58 RNA genes was sequenced as part of the DOE funded Community Sequencing Program (CSP) 2010 at the Joint Genome Institute (JGI).

Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Held, Brittany [Los Alamos National Laboratory (LANL); Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Hammon, Nancy [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Potter, Gabriele [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Land, Miriam L [ORNL; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

253

E-Print Network 3.0 - ammonia-oxidizing bacterium nitrosomonas...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Division, NASA Ames Research Center Collection: Environmental Sciences and Ecology 79 Land application of manure may contribute endocrine disrupting compounds (EDCs) such as...

254

E-Print Network 3.0 - anaerobic bacterium isolated Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

colonies with visually distinct morphologies were restreaked several times on R2A agar... plates. Pure bacterial colonies, picked from each isolate, were boiled in 50 l...

255

Complete Genome Sequence of the hyperthermophilic sulfate-reducing bacterium Thermodesulfobacterium geofontis OPF15T  

SciTech Connect (OSTI)

Thermodesulfobacterium geofontis OPF15T was isolated from Obsidian Pool, Yellowstone National Park and grows optimally at 83 oC. The OPF15T genome was finished at the Joint Genome Institute and the 1.6 Mb sequence has been annotated and deposited for future genomic studies aimed at understanding microbial processes and nutrient cycles in high-temperature environments.

Elkins, James G [ORNL; Hamilton-Brehm, Scott [ORNL; Walston Davenport, Karen [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Meincke, Linda [Los Alamos National Laboratory (LANL); Detter, J C [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Cottingham, Robert W [ORNL

2013-01-01T23:59:59.000Z

256

E-Print Network 3.0 - anaerobic acid-resistant bacterium Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Quantification of Methanogenic Groups in Anaerobic... February 1994 The microbial community structure of anaerobic biological reactors was evaluated by using... ,...

257

E-Print Network 3.0 - anaerobic thermohalophilic bacterium Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Quantification of Methanogenic Groups in Anaerobic... February 1994 The microbial community structure of anaerobic biological reactors was evaluated by using... ,...

258

E-Print Network 3.0 - anaerobic bacterium anaerocellum Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Quantification of Methanogenic Groups in Anaerobic... February 1994 The microbial community structure of anaerobic biological reactors was evaluated by using... ,...

259

An Oleaginous Bacterium That Intrinsically Accumulates Long-Chain Free Fatty Acids in its Cytoplasm  

Science Journals Connector (OSTI)

...biodiesel production. INTRODUCTION Fatty acids with medium-length...practicality and yield of biodiesel production. Published...coli: implications for biodiesel production. Metab...fatty-acid-derived fuels and chemicals from plant...BF Pfleger. 2012. Engineering Escherichia coli to...

Taiki Katayama; Manabu Kanno; Naoki Morita; Tomoyuki Hori; Takashi Narihiro; Yasuo Mitani; Yoichi Kamagata

2013-12-02T23:59:59.000Z

260

Coaggregation by the Freshwater Bacterium Sphingomonas natatoria Alters Dual-Species Biofilm Formation  

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...180 min were the occasional larger glass-attached clusters observed...continued to develop to form large stacks of biofilm cells that...diversity in kerosene-based drilling fluid from the deep ice borehole at Vostok, East Antarctica...

K. R. Min; A. H. Rickard

2009-04-17T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Multiple genome sequences reveal adaptations of a phototrophic bacterium to sediment microenvironments  

Science Journals Connector (OSTI)

...flank the cluster of BisB18 genes that codes for a carbon monoxide dehydrogenase...7). 1 Coleman ML Chisholm SW ( 2007 ) Code and context: Prochlorococcus as a model...possible DNA-binding protein hu-alpha (NS2) (HU-2) RPA2954 pepT nha:Nham...

Yasuhiro Oda; Frank W. Larimer; Patrick S. G. Chain; Stephanie Malfatti; Maria V. Shin; Lisa M. Vergez; Loren Hauser; Miriam L. Land; Stephan Braatsch; J. Thomas Beatty; Dale A. Pelletier; Amy L. Schaefer; Caroline S. Harwood

2008-01-01T23:59:59.000Z

262

Complete Genome Sequence of the Marine, Chemolithoautotrophic, Ammonia-Oxidizing Bacterium Nitrosococcus oceani ATCC 19707  

Science Journals Connector (OSTI)

...hypothetical genes; pale green, structural RNAs...electron acceptors (green). The two inner circles...their involvement in energy metabolism (ATP, pmf...Springer Science and Business Media, Inc., New York...oxidation of ammonia as an energy source in bacteria in...

Martin G. Klotz; Daniel J. Arp; Patrick S. G. Chain; Amal F. El-Sheikh; Loren J. Hauser; Norman G. Hommes; Frank W. Larimer; Stephanie A. Malfatti; Jeanette M. Norton; Amisha T. Poret-Peterson; Lisa M. Vergez; Bess B. Ward

2006-09-01T23:59:59.000Z

263

Novel Denitrifying Bacterium Ochrobactrum anthropi YD50.2 Tolerates High Levels of Reactive Nitrogen Oxides  

Science Journals Connector (OSTI)

...was replaced with argon gas by purging for 15 min...production was analyzed by gas chromatography as described...to easily visualize N2 bubbles generated by denitrification...exopolysaccharide in activated sludge. Bioresour. Technol...production in high-strength wastewater. Water Res...

Yuki Doi; Naoki Takaya; Noboru Takizawa

2009-06-19T23:59:59.000Z

264

Reuters AlertNet -Genome map shows how bacterium gobbles radiation Get a password  

E-Print Network [OSTI]

strategies and biotechnologies for cleaning up groundwater at DOE (the Department of Energy) and at industry a comprehensive picture that has led to fundamental changes in how scientists evaluate this microbe," said Barbara

Lovley, Derek

265

Science Blog -Bacterium cleans up uranium, generates electricity Create an account  

E-Print Network [OSTI]

electrons and "reduce" metal ions as part of its energy-generating metabolism. From DOE/U.S. Department and biotechnologies for cleaning up groundwater at DOE and at industry sites." The contamination of groundwater that has led to fundamental changes in how scientists evaluate this microbe," said Barbara Methe, the TIGR

Lovley, Derek

266

The Aquatic Budding Bacterium Blastobacter denitrificans Is a Nitrogen-Fixing Symbiont of Aeschynomene indica  

Science Journals Connector (OSTI)

...replications, and five jars without inoculated bacteria served as controls. The plants were grown in a greenhouse without supplemental lighting for 34 days during July and August. The plants were uprooted, and the tops were cut off to determine...

Peter van Berkum; Bertrand D. Eardly

2002-03-01T23:59:59.000Z

267

E-Print Network 3.0 - acid bacterium granulibacter Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

orientation, o Summary: FoodsAssociated Foods Low-acid canned foods Meat Poultry Seafood Vegetables Herb or garlic infused oil... Potatoes baked in foil Honey and corn syrup...

268

Biodegradation of bisphenol A and other bisphenols by a gram-negative aerobic bacterium.  

Science Journals Connector (OSTI)

...chemical plant's biotreatment...sludge from a wastewater treatment plant at a plastics...Carbon balance analysis...identified by mass spectroscopy...taken from a wastewater treatment facility...manufacturing plant was enriched...

J H Lobos; T K Leib; T M Su

1992-06-01T23:59:59.000Z

269

E-Print Network 3.0 - active bacterium phylogenetically Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Biological Engineering, Ohio State University Collection: Renewable Energy 14 J. theor. Biol. (1999) 196, 251261 Article No. jtbi.1998.0838, available online at...

270

E-Print Network 3.0 - aquatic bacterium caulobacter Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

de Physique du Globe de Paris Collection: Geosciences 38 Diffley, J.F. (2004). Curr. Biol. 14, R778R786. Geng, Y., Yu, Q., Sicinska, E., Das, M., Schneider, J.E., Bhat-...

271

E-Print Network 3.0 - ammonia-oxidizing bacterium nitrosococcus...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Collection: Chemistry 93 General Discussion Chapter 9: General Discussion Summary: . Soil Biol Biochem 23: 717-723. Hermansson A & Lindgren P-E (2001) Quantification of...

272

E-Print Network 3.0 - acid bacterium isolated Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Physics, Wageningen Universiteit Collection: Physics ; Biology and Medicine 11 J. theor. Biol. (1999) 196, 251261 Article No. jtbi.1998.0838, available online at http:...

273

Reconstitution of a Thermostable Xylan-Degrading Enzyme Mixture from the Bacterium Caldicellulosiruptor bescii  

Science Journals Connector (OSTI)

...chains, such as arabinose, glucuronic acid, acetate, and ferulic acid (8). Current methods for pretreatment of biomass feedstocks include dilute acid pretreatment, which destroys much of the hemicellulose (9), and ammonia fiber explosion...

Xiaoyun Su; Yejun Han; Dylan Dodd; Young Hwan Moon; Shosuke Yoshida; Roderick I. Mackie; Isaac K. O. Cann

2012-12-21T23:59:59.000Z

274

Solvent Stress Response of the Denitrifying Bacterium “Aromatoleum aromaticum” Strain EbN1  

Science Journals Connector (OSTI)

...9 AcnA2 Aconitase A2 1.8 4.5 General stress-related proteins BetB Betaine...m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer...in oxidative (e.g., SodB) and general (e.g., ClpB) stress responses...

Kathleen Trautwein; Simon Kühner; Lars Wöhlbrand; Thomas Halder; Kenny Kuchta; Alexander Steinbüchel; Ralf Rabus

2008-02-08T23:59:59.000Z

275

Rapid Aggregation of Biofuel-Producing Algae by the Bacterium Bacillus sp. Strain RP1137  

Science Journals Connector (OSTI)

...fuels have provided the energy to drive development...modern world. Most of the energy used in the United States...natural gas (1). Our energy infrastructure, particularly...has been spurred by the Renewable Fuel Standard, which...disadvantages with respect to cost, throughput, and posttreatment...

Ryan J. Powell; Russell T. Hill

2013-07-26T23:59:59.000Z

276

Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae  

Science Journals Connector (OSTI)

...freshwater ponds in Buenos Aires, Argentina (Fig. 2 and 3, and see...a fellow at the CONICET, Argentina. This work was supported...2009. The water footprint of bioenergy. Proc. Natl. Acad. Sci...Biologicas, FIBA, Mar del Plata, Argentina. | Journal Article Research...

Juan Cesar Federico Ortiz-Marquez; Mauro Do Nascimento; Maria de los Angeles Dublan; Leonardo Curatti

2012-01-20T23:59:59.000Z

277

Rapid Aggregation of Biofuel-Producing Algae by the Bacterium Bacillus sp. Strain RP1137  

Science Journals Connector (OSTI)

...from fossil fuels, including crude oil, coal, and natural gas (1). Our energy infrastructure...that these challenges are technical and economic in nature, as the process of creating...JW , MD Johnson and JL Outlaw. 2012. Economic comparison of open pond raceways to photo...

Ryan J. Powell; Russell T. Hill

2013-07-26T23:59:59.000Z

278

The oxidation of ethylene glycol by a salt-requiring bacterium  

E-Print Network [OSTI]

sources Authentication of 1, 2-14C-ethylene glycol Growth measurements Growth studies Respiration studies Respiration of ethylene glycol, glycolic acid, and glyoxylic acid Respiration of 1, 2-14C-ethylene glycol Enzyme assays Identification... of labelled compounds from cell-free preparations exposed to 14C-ethylene glycol RESULTS AND DISCUSSION Growth studies Respiration studies Respiration of ethylene glycol, glycolic acid, and glyoxylic acid Respiration of 1, 2-14C-ethylene glycol Enzyme...

Caskey, William Horton

2012-06-07T23:59:59.000Z

279

Proteomic Analysis of Stationary Phase in the Marine Bacterium 'Candidatus Pelagibacter ubique'  

SciTech Connect (OSTI)

Candidatus Pelagibacter ubique, an abundant marine alphaproteobacterium, subsists in nature at low ambient nutrient concentrations and may often be exposed to nutrient limitation, but its genome revealed no evidence of global regulatory adaptations to stationary phase. We used high-resolution capillary liquid chromatography (LC) coupled online to an LTQ mass spectrometer to build an Accurate Mass and Time (AMT) tag library, and employed the AMT tag approach to quantitatively examine proteome differences between exponentially growing and stationary phase Cand. P. ubique cells cultivated in a seawater medium. The AMT tag library represented 72% of the predicted protein coding genes. Stationary phase protein abundance increased for OsmC, which mitigates oxidative damage, and for molecular chaperones, enzymes involved in methionine and cysteine biosynthesis, proteins involved in rho-dependent transcription termination, and the signal transduction enzymes CheY-FisH and ChvG. Our findings indicate that Cand. P. ubique responds adaptively to stationary phase by increasing the abundance of a suite of proteins that contribute to homeostasis, but does not undergo major proteome remodeling. We speculate that this limited response may enable Cand. P. ubique to cope with ambient conditions in which nutrients are often insufficient for short periods, and the ability to resume growth overrides the capacity for long term survival afforded by more comprehensive global stationary phase responses.

Sowell, Sarah M.; Norbeck, Angela D.; Lipton, Mary S.; Nicora, Carrie D.; Callister, Stephen J.; Smith, Richard D.; Barofsky, Douglas F.; Giovannoni, Stephen J.

2008-05-01T23:59:59.000Z

280

A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163  

Science Journals Connector (OSTI)

...compounds affecting wine quality or safety such as...response of O. oeni in wine uses principally three...bicarbonate (Merck). To increase the proportion of...acid bacteria in the quality improvement and depreciation of wine. Ant. van Leeuwenhoek...

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Association with an Ammonium-Excreting Bacterium Allows Diazotrophic Culture of Oil-Rich Eukaryotic Microalgae  

Science Journals Connector (OSTI)

...depletion of the world's reserves of oil and...sources of energy during the...sources of energy in the framework...depletion of the world's reserves of oil and...depletion of the world's reserves of oil and...sources of energy during the...

Juan Cesar Federico Ortiz-Marquez; Mauro Do Nascimento; Maria de los Angeles Dublan; Leonardo Curatti

2012-01-20T23:59:59.000Z

282

Memory in Microbes: Quantifying History-Dependent Behavior in a Bacterium  

E-Print Network [OSTI]

4. Casadesus J, D'Ari R (2002) Memory in bacteria and phage.2004) Switches, cross?talk and memory in Escherichia coli2007) Rational design of memory in eukaryotic cells. Genes

Wolf, Denise M.

2009-01-01T23:59:59.000Z

283

Memory in microbes: quantifying history-Dependent behavior in a bacterium.  

E-Print Network [OSTI]

4. Casadesus J, D'Ari R (2002) Memory in bacteria and phage.2004) Switches, cross-talk and memory in Escherichia coli2007) Rational design of memory in eukaryotic cells. Genes

Wolf, Denise M.

2010-01-01T23:59:59.000Z

284

E-Print Network 3.0 - anaerobic bacterium thiobacillus Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

14 Environmental Microbiology (2002) 4(9), 510-516 Multip,le influences of nitrate on uranium solubility Summary: of organic compounds on the growth of the chemolithotrophic...

285

E-Print Network 3.0 - anoxygenic phototrophic bacterium Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Research Group Collection: Biology and Medicine 3 Origin and evolution of photosynthesis: clues from genome comparison Armen Y. Mulkidjanian1,2 Summary: of a reaction...

286

Functional Heterologous Expression of an Engineered Full Length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum  

SciTech Connect (OSTI)

BACKGROUND: Cellulose is highly recalcitrant and thus requires a specialized suite of enzymes to solubilize it into fermentable sugars. In C. thermocellum, these extracellular enzymes are present as a highly active multi-component system known as the cellulosome. This study explores the expression of a critical C. thermocellum cellulosomal component in T. saccharolyticum as a step toward creating a thermophilic bacterium capable of consolidated bioprocessing by employing heterologously expressed cellulosomes. RESULTS: We developed an inducible promoter system based on the native T. saccharolyticum xynA promoter, which was shown to be induced by xylan and xylose. The promoter was used to express the cellulosomal component cipA*, an engineered form of the wild-type cipA from C. thermocellum. Expression and localization to the supernatant were both verified for CipA*. When a cipA mutant C. thermocellum strain was cultured with a CipA*-expressing T. saccharolyticum strain, hydrolysis and fermentation of 10 grams per liter SigmaCell 101, a highly crystalline cellulose, were observed. This trans-species complementation of a cipA deletion demonstrated the ability for CipA* to assemble a functional cellulosome. CONCLUSION: This study is the first example of an engineered thermophile heterologously expressing a structural component of a cellulosome. To achieve this goal we developed and tested an inducible promoter for controlled expression in T. saccharolyticum as well as a synthetic cipA. In addition, we demonstrate a high degree of hydrolysis (up to 93%) on microcrystalline cellulose.

Currie, Devin [Dartmouth College; Herring, Christopher [Mascoma Corporation; Guss, Adam M [ORNL; Olson, Daniel G. [Thayer School of Engineering at Dartmouth; Hogsett, David [Mascoma Corporation; Lynd, Lee R [Thayer School of Engineering at Dartmouth

2013-01-01T23:59:59.000Z

287

Laboratory Directed Research & Development program. Annual report to the Department of Energy  

SciTech Connect (OSTI)

This report briefly discusses the following projects coordinated at Brookhaven National Laboratory: investigation of the utility of max-entropy methods for the analysis of powder diffraction data; analysis of structures and interactions of nucleic acids and proteins by small angle x-ray diffraction; relaxographic MRI and functional MRI; very low temperature infra-red laser absorption as a potential analytical tool; state-resolved measurements of H{sub 2} photodesorption: development of laser probes of H{sub 2} for in-situ accelerator measurements; Siberian snake prototype development for RHIC; synthesis and characterization of novel microporous solids; ozone depletion, chemistry and physics of stratospheric aerosols; understanding the molecular basis for the synthesis of plant fatty acids possessing unusual double bond positions; structure determination of outer surface proteins of the Lyme disease spirochete; low mass, low-cost multi-wire proportional chambers for muon systems of collider experiments; theory of self-organized criticality; development of the PCR-SSCP technique for the detection, at the single cell level, of specific genetic changes; feasibility of SPECT in imaging of F-18 FDG accumulation in tumors; visible free electron laser oscillator experiment; study of possible 2 + 2 TeV muon-muon collider; ultraviolet FEL R & D; precision machining using hard x-rays; new directions in in-vivo enzyme mapping: catechol-O-methyltransferase; proposal to develop a high rate muon polarimeter; development of intense, tunable 20-femtosecond laser systems; use of extreme thermophilic bacterium thermatoga maritima as a source of ribosomal components and translation factors for structural studies; and biochemical and structural studies of Chaperon proteins from thermophilic bacteria and other experiments.

Ogeka, G.J.; Romano, A.J.

1995-12-01T23:59:59.000Z

288

Life and hydrothermal vents  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Life and hydrothermal vents Life and hydrothermal vents Name: williamh Status: N/A Age: N/A Location: N/A Country: N/A Date: Around 1993 Question: Are there biological communities near hydrothermal vents in the ocean? Is there any life inside the hydrothermal vent? Replies: If the presence of microorganisms in hot springs and geysers are any indication, I am certain there is life inside hydrothermal vents. These heat loving organisms are termed "thermophiles" and thrive where other life dies. They are able to survive in extreme heat due to the unique way their proteins are synthesized. The May 1993 Discover has a special article on thermophiles. wizkid Life at high temperature became very interesting to molecular biologists recently. The enormously useful technique known as PCR, (polymerase chain reaction), by which very small amounts of rare DNA can be amplified to large concentrations (Jurassic Park!), depends on having a DNA polymerase (the enzyme that synthesizes complementary DNA strands during replication of chromosomes), that can work at high temperatures, or at least can survive repeated high temperature cycles. PCR depends on synthesis of DNA followed by forced separation of the daughter strands at high temperature, followed by new synthesis, to amplify DNA exponentially. At any rate, normal bacterial polymerase will not work because the high temperature cycles kill it. Enter the now infamous, patented Taq polymerase, isolated from Thermus aquaticus, a hot spring bacterium, which works after heating to up to 94 C! So knowledge of life at high temperature allowed molecular biologists to get PCR to work, with all its benefits in cloning very rare genes and amplifying small amounts of DNA for forensic work etc.

289

High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10  

Science Journals Connector (OSTI)

...Cairo 12622, Egypt. # Alexander Steinbuchel...microorganisms, are energy-efficient processes...and which utilize renewable resources such as...Fornasiero (ed.), Renewable resources and renewable energy: a global challenge...

Mohammad H. A. Ibrahim; Alexander Steinbüchel

2010-10-01T23:59:59.000Z

290

Characterization of a thermophilic sulfur oxidizing enrichment culture dominated by a Sulfolobus sp. obtained from an underground hot spring for use in extreme bioleaching conditions  

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A thermoacidophilic elemental sulfur and chalcopyrite oxidizing enrichment culture VS2 was obtained from hot spring run-off sediments of an underground mine. It contained only archaeal species, namely a Sulfolobu...

Virpi L. A. Salo-Zieman; Tarja Sivonen…

2006-12-01T23:59:59.000Z

291

Ethanol Production by Thermophilic Bacteria: Relationship Between Fermentation Product Yields of and Catabolic Enzyme Activities in Clostridium thermocellum and Thermoanaerobium brockii  

Science Journals Connector (OSTI)

...similar end products (ethanol, H2/CO2, lactate...including the range of energy sources metabolized...thermocellum grows on ethanol as energy source in co-culture...cellulosic biomass to ethanol, p. 61-67. In...3rd Annual Biomass Energy Systems Conference...

R. Lamed; J. G. Zeikus

1980-11-01T23:59:59.000Z

292

Monitoring a genetically engineered bacterium in a freshwater environment by rapid enzymatic amplification of a synthetic DNA “number-plate”  

Science Journals Connector (OSTI)

In order to set up a sensitive and reliable detection method to monitor environmentally released genetically engineered microorganisms (GEMs) a 72-bp, double-stranded DNA fragment has been built by annealing and ...

Augusto Amici; Marco Bazzicalupo; Enzo Gallori…

1991-11-01T23:59:59.000Z

293

Stereospecific Biotransformation of Dihydrodaidzein into (3S)-Equol by the Human Intestinal Bacterium Eggerthella Strain Julong 732  

Science Journals Connector (OSTI)

...respectively obtained at 600 and 150 MHz on an Avance 600 NMR spectrometer...United Kingdom) under an atmosphere of 5 CO2, 10 H2, and 85 N2...in GAM under the anaerobic atmosphere were examined. Under the experimental...Dietary isoflavones reduce plasma cholesterol and atherosclerosis...

Mihyang Kim; Su-Il Kim; Jaehong Han; Xiu-Ling Wang; Dae-Geun Song; Soo-Un Kim

2009-03-20T23:59:59.000Z

294

Functional Genomics with a Comprehensive Library of Transposon Mutants for the Sulfate-Reducing Bacterium Desulfovibrio alaskensis G20  

Science Journals Connector (OSTI)

...not included in the average. In this paper...and Ng TK. 1988. Effect of phosphate on the...Rapp-Giles BJ, Price MN, Lin M, Bruce...ED, Jorgens D, Price MN, Witkowska HE...Wall JD. 2010. Effect of the deletion of...1160446 . 34. Price MN , Deutschbauer...

Jennifer V. Kuehl; Morgan N. Price; Jayashree Ray; Kelly M. Wetmore; Zuelma Esquivel; Alexey E. Kazakov; Michelle Nguyen; Raquel Kuehn; Ronald W. Davis; Terry C. Hazen; Adam P. Arkin; Adam Deutschbauer

2014-06-01T23:59:59.000Z

295

Low Relative Abundances of the Mucolytic Bacterium Akkermansia muciniphila and Bifidobacterium spp. in Feces of Children with Autism  

Science Journals Connector (OSTI)

...participants using: Gluten- and casein-free diet Casein-free...8 a ASD, autism spectrum...children with autism. Future...implementing gluten-free and/or casein-free diets. Also...implementing a gluten-free and/or casein-free diet. The study...resembling autism (e.g...

Lv Wang; Claus T. Christophersen; Michael J. Sorich; Jacobus P. Gerber; Manya T. Angley; Michael A. Conlon

2011-07-22T23:59:59.000Z

296

Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET  

E-Print Network [OSTI]

FoF1-ATP synthase is the ubiquitous membrane-bound enzyme in mitochondria, chloroplasts and bacteria which provides the 'chemical energy currency' adenosine triphosphate (ATP) for cellular processes. In Escherichia coli ATP synthesis is driven by a proton motive force (PMF) comprising a proton concentration difference {\\Delta}pH plus an electric potential {\\Delta}{\\Psi} across the lipid membrane. Single-molecule in vitro experiments have confirmed that proton-driven subunit rotation within FoF1-ATP synthase is associated with ATP synthesis. Based on intramolecular distance measurements by single-molecule fluorescence resonance energy transfer (FRET) the kinetics of subunit rotation and the step sizes of the different rotor parts have been unraveled. However, these experiments were accomplished in the presence of a PMF consisting of a maximum {\\Delta}pH ~ 4 and an unknown {\\Delta}{\\Psi}. In contrast, in living bacteria the maximum {\\Delta}pH across the plasma membrane is likely 0.75, and {\\Delta}{\\Psi} has bee...

Seyfert, Karin; Yaginuma, Hideyuki; Ernst, Stefan; Noji, Hiroyuki; Iino, Ryota; Boersch, Michael

2011-01-01T23:59:59.000Z

297

Diffusion properties of single FoF1-ATP synthases in a living bacterium unraveled by localization microscopy  

E-Print Network [OSTI]

FoF1-ATP synthases in Escherichia coli (E. coli) bacteria are membrane-bound enzymes which use an internal proton-driven rotary double motor to catalyze the synthesis of adenosine triphosphate (ATP). According to the 'chemiosmotic hypothesis', a series of proton pumps generate the necessary pH difference plus an electric potential across the bacterial plasma membrane. These proton pumps are redox-coupled membrane enzymes which are possibly organized in supercomplexes, as shown for the related enzymes in the mitochondrial inner membrane. We report diffusion measurements of single fluorescent FoF1-ATP synthases in living E. coli by localization microscopy and single enzyme tracking to distinguish a monomeric enzyme from a supercomplex-associated form in the bacterial membrane. For quantitative mean square displacement (MSD) analysis, the limited size of the observation area in the membrane with a significant membrane curvature had to be considered. The E. coli cells had a diameter of about 500 nm and a length o...

Renz, Marc; Boersch, Michael

2012-01-01T23:59:59.000Z

298

Complete genome sequence of the filamentous gliding predatory bacterium Herpetosiphon aurantiacus type strain (114-95T)  

SciTech Connect (OSTI)

Herpetosiphon aurantiacus Holt and Lewin 1968 is the type species of the genus Herpetosiphon, which in turn is the type genus of the family Herpetosiphonaceae, type family of the order Herpe- tosiphonales in the phylum Chloroflexi. H. aurantiacus cells are organized in filaments which can rapidly glide. The species is of interest not only because of its rather isolated position in the tree of life, but also because Herpetosiphon ssp. were identified as predators capable of facultative pre- dation by a wolf pack strategy and of degrading the prey organisms by excreted hydrolytic en- zymes. The genome of H. aurantiacus strain 114-95T is the first completely sequenced genome of a member of the family Herpetosiphonaceae. The 6,346,587 bp long chromosome and the two 339,639 bp and 99,204 bp long plasmids with a total of 5,577 protein-coding and 77 RNA genes was sequenced as part of the DOE Joint Genome Institute Program DOEM 2005.

Kiss, Hajnalka [Los Alamos National Laboratory (LANL); Nett, Markus [Hans Knöll Institute, Jena, Germany; Domin, Nicole [Hans Knöll Institute, Jena, Germany; Martin, Karin [Hans Knöll Institute, Jena, Germany; Maresca, Julia A. [Pennsylvania State University, University Park, PA; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Berry, Kerrie W. [United States Department of Energy Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Richardson, P M [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Schmutz, Jeremy [Stanford University; Brettin, Thomas S [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bryant, Donald A. [Pennsylvania State University, University Park, PA

2011-01-01T23:59:59.000Z

299

Genome sequence of the flexirubin-pigmented soil bacterium Niabella soli type strain (JS13-8T)  

SciTech Connect (OSTI)

Niabella soli Weon et al. 2008 is a member of the Chitinophagaceae, a family within the class Sphingobacteriia that is poorly characterized at the genome level, thus far. N. soli strain JS13-8T is of interest for its ability to produce a variety of glycosyl hydrolases. The ge- nome of N. soli strain JS13-8T is only the second genome sequence of a type strain from the family Chitinophagaceae to be published, and the first one from the genus Niabella. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,697,343 bp long chromosome with its 3,931 protein-coding and 49 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Anderson, Iain [U.S. Department of Energy, Joint Genome Institute; Munk, Christine [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Tindall, Brian [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Ivanova, N [U.S. Department of Energy, Joint Genome Institute

2012-01-01T23:59:59.000Z

300

Complete genome sequence of the marine, cellulose and xylan degrading bacterium Glaciecola sp. 4H-3-7+YE-5  

SciTech Connect (OSTI)

Glaciecola sp. 4H-3-7+YE-5 was isolated from deep sea sediments at Suruga Bay in Japan and is capable of efficiently hydrolyzing cellulose and xylan. The complete genome sequence of Glaciecola sp. 4H-3-7+YE-5 revealed several genes encoding putatively novel glycoside hydrolases associated with plant biomass degradation.

Klippel, Dr Barbara [Technische Universitat Hamburg-Harburg (Hamburg University of Technology); Bruce, David [Los Alamos National Laboratory (LANL); Davenport, Karen W. [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Han, Shunsheng [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Wiebusch, Sigrid [Technische Universitat Hamburg-Harburg (Hamburg University of Technology); Basner, Alexander [Technische Universitat Hamburg-Harburg (Hamburg University of Technology); Abe, Fumiyoshi [Japan Agency for Marine-Earth Science and Technology (JAMSTEC); Horikoshi, Koki [Japan Agency for Marine-Earth Science and Technology (JAMSTEC); Antranikian, Garabed [Technische Universitat Hamburg-Harburg (Hamburg University of Technology)

2011-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Evidence for Direct Electron Transfer by a Gram-Positive Bacterium Isolated from a Microbial Fuel Cell  

Science Journals Connector (OSTI)

...vol/vol) from microbial fuel cells. Effects on the rate...experiments with Shewanella algae strain BRY, Geothrix fermentans...glucose in mediatorless microbial fuel cells. Nat. Biotechnol. 21...electricity generation at microbial fuel cell anodes via excretion of...

K. C. Wrighton; J. C. Thrash; R. A. Melnyk; J. P. Bigi; K. G. Byrne-Bailey; J. P. Remis; D. Schichnes; M. Auer; C. J. Chang; J. D. Coates

2011-09-09T23:59:59.000Z

302

Caffeate Respiration in the Acetogenic Bacterium Acetobacterium woodii: a Coenzyme A Loop Saves Energy for Caffeate Activation  

Science Journals Connector (OSTI)

...derived from molecular hydrogen to the synthesis...such as molecular hydrogen, C1 compounds like...lithotrophic metabolism when hydrogen is the electron donor...Reduced ferredoxin then fuels the sodium-motive...by a gene of the car operon, carB (18...containing pET21a-carA. Cells were grown aerobically...

Verena Hess; José M. González; Anutthaman Parthasarathy; Wolfgang Buckel; Volker Müller

2013-01-11T23:59:59.000Z

303

Role of sortase-dependent pili of Bifidobacterium bifidum PRL2010 in modulating bacterium–host interactions  

Science Journals Connector (OSTI)

...full-length forms of microbial surface structures (28). AFM...Furthermore, the cell surface of (nisin-induced...adhesion promoting cell-surface molecules on PRL2010...experiment showed that treatment with Ab pil3 decreased...by the polysaccharide xylan (Fig. 3D...

Francesca Turroni; Fausta Serafini; Elena Foroni; Sabrina Duranti; Mary O’Connell Motherway; Valentina Taverniti; Marta Mangifesta; Christian Milani; Alice Viappiani; Tommaso Roversi; Borja Sánchez; Andrea Santoni; Laura Gioiosa; Alberto Ferrarini; Massimo Delledonne; Abelardo Margolles; Laura Piazza; Paola Palanza; Angelo Bolchi; Simone Guglielmetti; Douwe van Sinderen; Marco Ventura

2013-01-01T23:59:59.000Z

304

Identification and Characterization of a Novel Intracellular Alkaline ?-Amylase from the Hyperthermophilic Bacterium Thermotoga maritima MSB8  

Science Journals Connector (OSTI)

...1-h assay using starch as the substrate...also cleaved soluble starch (68%) and b-limit-dextrin...classified as an a-amylase (EC 3.2.1...which helps to break down a-glucan polysaccharides...this cytoplasmic a-amylase is significantly...

Meike Ballschmiter; Ole Fütterer; Wolfgang Liebl

2006-03-01T23:59:59.000Z

305

Porticoccus hydrocarbonoclasticus sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium Identified in Laboratory Cultures of Marine Phytoplankton  

Science Journals Connector (OSTI)

...and refined fossil fuels. Based on their...adsorption. Stock solutions (ca. 3,000...saturated NaCl solution were added to...which 30% of the air atmosphere was...2000. Oil pollution of marine algae...fractions. Water Air Soil Pollut...

Tony Gutierrez; Peter D. Nichols; William B. Whitman; Michael D. Aitken

2011-12-02T23:59:59.000Z

306

Sodium-dependent transport of neutral amino acids by whole cells and membrane vesicles of Streptococcus bovis, a ruminal bacterium.  

Science Journals Connector (OSTI)

...diluted into either lithium or choline chloride...was inhibited by an excess of threonine, but...diluted into either lithium or choline chloride...was inhibited by an excess of threonine, but...choline chloride, lithium chloride, or sodium...presence of a 70-fold excess of nonlabeled L-threonine...

J B Russell; H J Strobel; A J Driessen; W N Konings

1988-08-01T23:59:59.000Z

307

Differential effects of sodium on hydrogen- and glucose-dependent growth of the acetogenic bacterium Acetogenium kivui.  

Science Journals Connector (OSTI)

...0.012i FIG. 3. Effects of sodium, lithium, and potassium on the H2d dependent growth...represent the amount of biomass formed in excess of the inoculum. d Growth after a 3-day...represent the amount of biomass formed in excess of the inoculum. c Acetate (millimolar...

H C Yang; H L Drake

1990-01-01T23:59:59.000Z

308

Preferential Use of an Anode as an Electron Acceptor by an Acidophilic Bacterium in the Presence of Oxygen  

Science Journals Connector (OSTI)

...microbial fuel cell development, in which bacteria...separated from the cathode compartment, generally...of the microbial electrocatalyst (5, 8, 30...for the further development of microbial fuel...oxygen reduction cathodes. Biosens. Bioelectron...cells and their development. Biosens. Bioelectron...

Moustafa Malki; Antonio L. De Lacey; Nuria Rodríguez; Ricardo Amils; Victor M. Fernandez

2008-05-16T23:59:59.000Z

309

Evidence for Direct Electron Transfer by a Gram-Positive Bacterium Isolated from a Microbial Fuel Cell  

Science Journals Connector (OSTI)

...medium surrounding the anode was also evaluated...current-producing anodes (operated for 8...using BASi's Epsilon potentiostat and...was performed on anode culture medium and...electron transfer to iron(III) oxides by c-type cytochromes...

K. C. Wrighton; J. C. Thrash; R. A. Melnyk; J. P. Bigi; K. G. Byrne-Bailey; J. P. Remis; D. Schichnes; M. Auer; C. J. Chang; J. D. Coates

2011-09-09T23:59:59.000Z

310

Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae  

Science Journals Connector (OSTI)

...VcCD00017918 FLH197666.01F DQ775140 type IV pilus assembly protein PilB VC2425 pilC NP_232055 VcCD00017884 FLH197628.01F DQ775141 type IV pilin biogenesis protein PilC VC2426 pilD NP_232056 VcCD00061226 FLH236445.01F DQ899522 leader peptidase...

Andreas Rolfs; Wagner R. Montor; Sang Sun Yoon; Yanhui Hu; Bhupinder Bhullar; Fontina Kelley; Seamus McCarron; Daniel A. Jepson; Binghua Shen; Elena Taycher; Stephanie E. Mohr; Dongmei Zuo; Janice Williamson; John Mekalanos; Joshua LaBaer

2008-01-01T23:59:59.000Z

311

Isolation and Characterization of a Subsurface Bacterium Capable of Growth on Toluene, Naphthalene, and Other Aromatic Compounds  

Science Journals Connector (OSTI)

...to harbor two plasmids larger than 100 kb. Restricted...to harbor two plasmids larger than 100 kb. Restricted...sediments harbored relatively large (>100 kb) plasmids...were obtained from a borehole designated C-10 drilled...ination of cores during drilling, bacteria were isolated...

J. K. Fredrickson; F. J. Brockman; D. J. Workman; S. W. Li; T. O. Stevens

1991-03-01T23:59:59.000Z

312

From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov.  

Science Journals Connector (OSTI)

...as sole sources of carbon and energy when assayed by the conventional...bacteria in crystallizer ponds from solar salterns. Appl. Environ...fingerprinting methods in a multipond solar saltern. Environ. Microbiol...soda-depositing Lake Natron, East Africa Rift Valley. Int. J. Syst...

María José León; Ana B. Fernández; Rohit Ghai; Cristina Sánchez-Porro; Francisco Rodriguez-Valera; Antonio Ventosa

2014-04-18T23:59:59.000Z

313

A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity  

Science Journals Connector (OSTI)

...SYBYL built-in version of DIANA (Version 2.2.1), which makes use of a distance-geometry algorithm. Two cycles of REDAC (Redundant Dihedral Angle Constraint algorithm) were applied to optimize a total of 500 starting structures to yield the 20...

Claudia A. Blindauer; Mark D. Harrison; John A. Parkinson; Andrea K. Robinson; Jennifer S. Cavet; Nigel J. Robinson; Peter J. Sadler

2001-01-01T23:59:59.000Z

314

Cytochrome c4 Can Be Involved in the Photosynthetic Electron Transfer System in the Purple Bacterium Rubrivivax gelatinosus  

Science Journals Connector (OSTI)

The plasmids introduced are incapable of replication in R. gelatinosus cells; thus, kanamycin resistans means incorporation of the plasmid into their genomic DNAs via a single crossover homologue recombination. ...

Makito Ohmine; Katsumi Matsuura; Keizo Shimada; Jean Alric; André Verméglio; Kenji V. P. Nagashima

2009-08-21T23:59:59.000Z

315

Draft Genome Sequence of Moritella dasanensis Strain ArB 0140, a Psychrophilic Bacterium Isolated from the Arctic Ocean  

Science Journals Connector (OSTI)

...isolated from the Arctic Ocean, exhibited ice-pitting...organism in the Arctic Ocean. Nucleotide sequence...described in this paper is the first version, AKXQ01000000...Delcher AL , KA Bratke, EC Powers, and SL Salzberg. 2007...isolated from the Arctic ocean. Int. J. Syst. Evol...

Sung Gu Lee; Hye Yeon Koh; Jun Hyuck Lee; Sung-Ho Kang; Hak Jun Kim

2012-10-01T23:59:59.000Z

316

Biological consequences of ancient gene acquisition and duplication in the large genome soil bacterium, ""solibacter usitatus"" strain Ellin6076  

SciTech Connect (OSTI)

Bacterial genome sizes range from ca. 0.5 to 10Mb and are influenced by gene duplication, horizontal gene transfer, gene loss and other evolutionary processes. Sequenced genomes of strains in the phylum Acidobacteria revealed that 'Solibacter usistatus' strain Ellin6076 harbors a 9.9 Mb genome. This large genome appears to have arisen by horizontal gene transfer via ancient bacteriophage and plasmid-mediated transduction, as well as widespread small-scale gene duplications. This has resulted in an increased number of paralogs that are potentially ecologically important (ecoparalogs). Low amino acid sequence identities among functional group members and lack of conserved gene order and orientation in the regions containing similar groups of paralogs suggest that most of the paralogs were not the result of recent duplication events. The genome sizes of cultured subdivision 1 and 3 strains in the phylum Acidobacteria were estimated using pulsed-field gel electrophoresis to determine the prevalence of the large genome trait within the phylum. Members of subdivision 1 were estimated to have smaller genome sizes ranging from ca. 2.0 to 4.8 Mb, whereas members of subdivision 3 had slightly larger genomes, from ca. 5.8 to 9.9 Mb. It is hypothesized that the large genome of strain Ellin6076 encodes traits that provide a selective metabolic, defensive and regulatory advantage in the variable soil environment.

Challacombe, Jean F [Los Alamos National Laboratory; Eichorst, Stephanie A [Los Alamos National Laboratory; Xie, Gary [Los Alamos National Laboratory; Kuske, Cheryl R [Los Alamos National Laboratory; Hauser, Loren [ORNL; Land, Miriam [ORNL

2009-01-01T23:59:59.000Z

317

Generation of nanoparticles from technical powder of the insecticidal bacterium Bacillus thuringiensis var. kurstaki for improving efficacy  

Science Journals Connector (OSTI)

The technical powder of a local isolate of Bacillus thuringiensis (Bt) var. kurstaki namely DOR Bt-1 was subjected to high pressure homogenisation (HPH) to obtain nanoparticles (NPs) ranging 32-1,106 nm as determined through dynamic light scattering. NPs of Bt were also characterised for shape and size through scanning electron microscopy, field emission scanning electron microscopy and transmission electron microscopy that helped in visualising the smallest nanoparticles, i.e., less than even 50 nm. The larger particles comprised mainly Bt spores with a length and width of 1,139 and 813 nm respectively. HPH showed little effect on the spore viability. NPs had a zeta potential above +30 and were stable, resulted in higher mortality of Helicoverpa armigera larvae at a lower dose. Alkali solubilisation of the NPs resulted in higher values of protein and endotoxin quantified through Lowry's method and ELISA respectively. This is indicative of an increased solubility of the toxin in the midgut alkaline fluid of the insect leading to higher mortality coupled with quicker kill. Thus Bt as NPs has brighter prospects for insect pest management with increased speed of kill at lower doses.

K. Santha Murthy; V. Vineela; P.S. Vimala Devi

2014-01-01T23:59:59.000Z

318

evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation ans a consolidated bioprocessing approach  

SciTech Connect (OSTI)

Abstract Background: The inherent recalcitrance of lignocellulosic biomass is one of the major economic hurdles for the production of fuels and chemicals from biomass. Additionally, lignin is recognized as having a negative impact on enzymatic hydrolysis of biomass, and as a result much interest has been placed on modifying the lignin pathway to improve bioconversion of lignocellulosic feedstocks. Results: Previous results showed down-regulation of the caffeic acid 3-O-methyl transferase (COMT) gene in the lignin pathway yielded switchgrass (Panicum virgatum) that was more susceptible to bioconversion after dilute acid pretreatment. Here we examined the response of these plant lines to milder pretreatment conditions with yeast-based SSF, CBP with Clostridium thermocellum, and fermentations with the cellulolytic extreme thermophiles, Caldicellulosiruptor bescii and Caldicellulosiruptor obsidiansis. Unlike the S. cerevisiae SSF conversions, fermentations of pretreated down-regulated COMT transgenic switchgrass with C. thermocellum showed an apparent inhibition of fermentation not observed in the wild-type switchgrass. This inhibition can be eliminated by hot water extraction of the pretreated biomass which resulted in superior conversion yield with transgenic versus wild-type switchgrass for C. thermocellum, also exceeding the yeast-based SSF yield. Further fermentation evaluation of the transgenic switchgrass indicated differential inhibition for the Caldicellulosiruptor strains, which could not be rectified by additional processing conditions. Gas chromatography-mass spectrometry metabolite profiling was used to examine the fermentation broth to elucidate the relative abundance of lignin derived aromatic compounds. The types and abundance of fermentation-derived lignin constituents varied between C. thermocellum and each of the Caldicellulosiruptor strains. Conclusions: The down-regulation of the COMT gene improves the bioconversion of switchgrass relative to the wild-type regardless of the pretreatment condition or fermentation microorganism. However, bacterial fermentations demonstrated strain-dependent sensitivity to the COMT transgenic biomass, likely due to additional soluble lignin pathway-derived constituents resulting from the COMT gene disruption. Removal of these inhibitory constituents permitted completion of fermentation by C. thermocellum, but not by the Caldicellulosiruptor strains. The reason for this difference needs to be explored further.

Yee, Kelsey L [ORNL; Rodriguez, Jr., Miguel [ORNL; Tschaplinski, Timothy J [ORNL; Engle, Nancy L [ORNL; Martin, Madhavi Z [ORNL; Fu, Chunxiang [Noble Foundation; Wang, Zeng-Yu [Noble Foundation; Hamilton-Brehm, Scott [ORNL; Mielenz, Jonathan R [ORNL

2012-01-01T23:59:59.000Z

319

Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Redirecting Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum Yu Deng a,c , Daniel G. Olson a,c , Jilai Zhou a,c , Christopher D. Herring a,b,c , A. Joe Shaw d , Lee R. Lynd a,b,c,n a Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA b Mascoma Corporation, Lebanon, NH 03766, USA c BioEnergy Science Center, Oak Ridge, TN 37830, USA d Novogy, Inc. Cambridge, MA 02138, USA a r t i c l e i n f o Article history: Received 24 August 2012 Received in revised form 6 November 2012 Accepted 16 November 2012 Available online 29 November 2012 Keywords: Clostridium thermocellum Ethanol yield Pyruvate kinase Malate shunt a b s t r a c t In Clostridium thermocellum, a thermophilic anaerobic bacterium able to rapidly ferment cellulose to ethanol, pyruvate kinase (EC 2.7.1.40) is absent based on both the genome sequence and enzymatic

320

gkq1281 1..15  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Insights Insights into plant biomass conversion from the genome of the anaerobic thermophilic bacterium Caldicellulosiruptor bescii DSM 6725 Phuongan Dam 1,2,3 , Irina Kataeva 2,3 , Sung-Jae Yang 2,3 , Fengfeng Zhou 1,2,3 , Yanbin Yin 1,2,3 , Wenchi Chou 1,3 , Farris L. Poole II 2,3 , Janet Westpheling 3,4 , Robert Hettich 3 , Richard Giannone 3 , Derrick L. Lewis 3,5 , Robert Kelly 3,5 , Harry J. Gilbert 2,6 , Bernard Henrissat 7 , Ying Xu 1,2,3, * and Michael W. W. Adams 2,3, * 1 Institute of Bioinformatics, 2 Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, 3 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831, 4 Department of Genetics, University of Georgia, Athens, GA 30602, 5 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, 6 Complex Carbohydrate Research Center, University

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Embargoed Deletion  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

PNAS proof PNAS proof Embargoed Deletion of Cel48S Q:1 from Clostridium thermocellum ; 2 Daniel G. Olson a,b,c , Shital A. Tripathi a,c , Richard J. Giannone c,d , Jonathan Lo b,c , Nicky C. Caiazza a,c , David A. Hogsett a,c , Robert Hettich c,d , Adam M. Guss b,c , Genia Dubrovsky b,c , and Lee R. Lynd a,b,c,e,1 a Mascoma Corporation, NH 03766; b Thayer School of Engineering and e Department of Biological Sciences, Dartmouth College, NH 03755; and c BioEnergy Science Center, d Oak Ridge National Laboratory, TN 37830 Q:3 Edited* by Lonnie O'Neal Ingram, University of Florida, Gainesville, FL, and approved August 16, 2010 (received for review April 9, 2010) Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cel- lulosome complex. Creation of knockout mutants for Cel48S (also known as CelS, S S , and S8), the most abundant cellulosome

322

Interactions of Endoglucanases with Amorphous Cellulose Films Resolved by Neutron Reflectometry and Quartz Crystal Microbalance with Dissipation Monitoring  

SciTech Connect (OSTI)

A study of the interaction of four endoglucanases with amorphous cellulose films by neutron reflectometry (NR) and quartz crystal microbalance with dissipation monitoring (QCM-D) is reported. The endoglucanases include a mesophilic fungal endoglucanase (Cel45A from H. insolens), a processive endoglucanase from a marine bacterium (Cel5H from S. degradans), and two from thermophilic bacteria (Cel9A from A. acidocaldarius and Cel5A from T. maritima). The use of amorphous cellulose is motivated by the promise of ionic liquid pretreatment as a second generation technology that disrupts the native crystalline structure of cellulose. The endoglucanases displayed highly diverse behavior. Cel45A and Cel5H, which possess carbohydrate-binding modules (CBMs), penetrated and digested within the bulk of the films to a far greater extent than Cel9A and Cel5A, which lack CBMs. While both Cel45A and Cel5H were active within the bulk of the films, striking differences were observed. With Cel45A, substantial film expansion and interfacial broadening were observed, whereas for Cel5H the film thickness decreased with little interfacial broadening. These results are consistent with Cel45A digesting within the interior of cellulose chains as a classic endoglucanase, and Cel5H digesting predominantly at chain ends consistent with its designation as a processive endoglucanase.

Cheng, Gang [Joint Bioenergy Institute; Liu, Zelin [Virginia Polytechnic Institute and State University (Virginia Tech); Kent, Michael S [Sandia National Laboratories (SNL); Majewski, Jaroslaw [Los Alamos National Laboratory (LANL); Michael, Jablin [Los Alamos National Laboratory (LANL); Jaclyn, Murton K [Sandia National Laboratories (SNL); Halbert, Candice E [ORNL; Datta, Supratim [Joint Bioenergy Institute; Chao, Wang [Virginia Polytechnic Institute and State University (Virginia Tech); Brown, Page [Sandia National Laboratories (SNL)

2012-01-01T23:59:59.000Z

323

Comparison of Chloroflexus aurantiacus strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically  

SciTech Connect (OSTI)

Chloroflexus aurantiacus J-10-fl is a thermophilic green bacterium, a filamentous anoxygenic phototroph, and the model organism of the phylum Chloroflexi. We applied high-throughput, liquid chromatography-mass spectrometry in a global quantitative proteomics investigation of C. aurantiacus cells grown under oxic (chemoorganoheterotrophically) and anoxic (photoorganoheterotrophically) redox states. Our global analysis identified 13,524 high-confidence peptides that matched to 1,286 annotated proteins, 242 of which were either uniquely identified or significantly increased in abundance under anoxic culture conditions. Fifty-three of the 242 proteins are previously characterized photosynthesis-related proteins, including chlorosome proteins, proteins involved in the bacteriochlorophyll biosynthesis, 3-hydroxypropionate (3-OHP) CO2 fixation pathway, and components of electron transport chains. The remaining 190 proteins have not previously been reported. Of these, five proteins were found to be encoded by genes from a novel operon and observed only in photoheterotrophically grown cells. These proteins candidates may prove useful in further deciphering the phototrophic physiology of C. aurantiacus and other filamentous anoxygenic phototrophs.

Cao, Li; Bryant, Donald A.; Schepmoes, Athena A.; Vogl, Kajetan; Smith, Richard D.; Lipton, Mary S.; Callister, Stephen J.

2012-01-17T23:59:59.000Z

324

Cellulase, Clostridia, and Ethanol  

Science Journals Connector (OSTI)

...dependence on petroleum for energy. Coculture of a cellulolytic...promising approach to an alternate energy source from an economic viewpoint...University, Madison, NJ 07940, USA. ademain@drew.edu | Journal...enzymology Coculture Techniques Energy Metabolism Ethanol pharmacology

Arnold L. Demain; Michael Newcomb; J. H. David Wu

2005-03-01T23:59:59.000Z

325

BESC knowledgebase public portal  

Science Journals Connector (OSTI)

......microbes for alternative fuel solutions (Farrell...biomass for cellulolytic degradation by enzymes and organisms...of biomass and plant cell wall formation, degradation and biofuel production...Bio-ethanol - the fuel of tomorrow from the......

Mustafa H. Syed; Tatiana V. Karpinets; Morey Parang; Michael R. Leuze; Byung H. Park; Doug Hyatt; Steven D. Brown; Steve Moulton; Michael D. Galloway; Edward C. Uberbacher

2012-03-01T23:59:59.000Z

326

Microbial Cellulose Utilization: Fundamentals and Biotechnology  

Science Journals Connector (OSTI)

...the cellulolytic enzyme system of the processing organism...223, 348, 678), steam explosion (546, 581...organisms. Important tools for understanding microbial...been developed. Such tools include systems that allow foreign genes...

Lee R. Lynd; Paul J. Weimer; Willem H. van Zyl; Isak S. Pretorius

2002-09-01T23:59:59.000Z

327

Production, purification, and properties of a lipase from a bacterium (Pseudomonas aeruginosa YS-7) capable of growing in water-restricted environments.  

Science Journals Connector (OSTI)

...Article Production, purification, and properties...capable of growing in water-restricted environments...Lipase isolation & purification metabolism Pseudomonas...Substrate Specificity Water metabolism APPLIED...require addi- tional purification to reach ultimate...accompanied the HIC when water was used as final...

Y Shabtai; N Daya-Mishne

1992-01-01T23:59:59.000Z

328

Dynamics of Metabolic Activities and Gene Expression in the Roseobacter Clade Bacterium Phaeobacter sp. Strain MED193 during Growth with Thiosulfate  

Science Journals Connector (OSTI)

...Table 1 were designed using the Primer3Plus web interface (54) on the basis of the genome...using 3H-leucine. Mar. Microb. Food Webs 6 :107-114. 53. Parsons, TR , Y Maita...Leunissen. 2007. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res...

Saraladevi Muthusamy; Federico Baltar; José M. González; Jarone Pinhassi

2014-08-29T23:59:59.000Z

329

Complete Genome Sequence of the Marine Cellulose-and Xylan-Degrading Bacterium Glaciecola sp. Strain 4H-3-7+YE-5  

E-Print Network [OSTI]

of Technology, Hamburg, Germany  Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA   Los Alamos the DOE Office of Science. Oak Ridge National Laboratory is

Klippel, Barbara

2013-01-01T23:59:59.000Z

330

Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1  

Science Journals Connector (OSTI)

The high-resolution crystal structure of FfIBP was determined and the structure showed an intramolecular disulfide bond in the capping head loop region and a T-A/G-X-T/N ice-binding motif. The rigid capping head loop region and greater surface area of the ice-binding site are important for the antifreeze activity of FfIBP.

Do, H.

2014-03-19T23:59:59.000Z

331

Complete Genome Sequence of the Marine Cellulose-and Xylan-Degrading Bacterium Glaciecola sp. Strain 4H-3-7+YE-5  

E-Print Network [OSTI]

Len Pennacchio 4 , Sam Pitluck 4 , Roxanne Tapia 3 , Tanja Len Pennacchio 4 , Sam Pitluck 4 , Roxanne Tapia 3 ,  Tanja

Klippel, Barbara

2013-01-01T23:59:59.000Z

332

Complete genome sequence of the halophilic bacterium Spirochaeta africana type strain (Z-7692T) from the alkaline Lake Magadi in the East African Rift  

SciTech Connect (OSTI)

Spirochaeta africana Zhilina et al. 1996 is an anaerobic, aerotolerant, spiral-shaped bacte- rium that is motile via periplasmic flagella. The type strain of the species, Z-7692T, was iso- lated in 1993 or earlier from a bacterial bloom in the brine under the trona layer in a shallow lagoon of the alkaline equatorial Lake Magadi in Kenya. Here we describe the features of this organism, together with the complete genome sequence, and annotation. Considering the pending reclassification of S. caldaria to the genus Treponema, S. africana is only the second 'true' member of the genus Spirochaeta with a genome-sequenced type strain to be pub- lished. The 3,285,855 bp long genome of strain Z-7692T with its 2,817 protein-coding and 57 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Abt, Birte [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Scheuner, Carmen [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Held, Brittany [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Deshpande, Shweta [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Tindall, Brian [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute

2013-01-01T23:59:59.000Z

333

The Genome Sequence of Psychrobacter arcticus 273-4, a Psychroactive Siberian Permafrost Bacterium, Reveals Mechanisms for Adaptation to Low-Temperature Growth  

Science Journals Connector (OSTI)

...important factor in cold adaptation is the synthesis of wax esters. Wax esters are commonly found in plants and animals (30). Acinetobacter sp. accumulates large amounts of wax esters that are used later as a carbon source for growth...

Héctor L. Ayala-del-Río; Patrick S. Chain; Joseph J. Grzymski; Monica A. Ponder; Natalia Ivanova; Peter W. Bergholz; Genevive Di Bartolo; Loren Hauser; Miriam Land; Corien Bakermans; Debora Rodrigues; Joel Klappenbach; Dan Zarka; Frank Larimer; Paul Richardson; Alison Murray; Michael Thomashow; James M. Tiedje

2010-02-12T23:59:59.000Z

334

Proteome-Based Comparative Analyses of Growth Stages Reveal New Cell Cycle-Dependent Functions in the Predatory Bacterium Bdellovibrio bacteriovorus  

Science Journals Connector (OSTI)

...to totally impair swimming and to abolish predation...increasingly large cytosolic pool of FtsZ short protofilaments...thereby minimizing energy losses. Moreover...between predation efficiency and a facultative...during which free-swimming attack-phase...

Mally Dori-Bachash; Bareket Dassa; Shmuel Pietrokovski; Edouard Jurkevitch

2008-10-03T23:59:59.000Z

335

The ultimate ethanol: Technoeconomic evaluation of ethanol manufacture, comparing yeast vs Zymomonas bacterium fermentations. [Zymomonas mobilis:a5; Saccharomyces cerevisiae:a6  

SciTech Connect (OSTI)

If ethanol could be produced at a low enough price to serve as the precursor to ethylene and butadiene, it and its derivatives could account for 159 billion lb, or 50% of the US production of 316 billion lb of synthetic organic chemicals, presently valued at $113 billion. This use would consume 3.4 billion bu of corn, or {approximately}40% of the corn crop. This study evaluates advance process engineering and genetic engineering techniques that could generate savings and reduce production costs. The most rewarding development strategy appears to be to demonstrate at pilot scale the use of immobilized Zymomonas mobilis bacteria in a fluidized-bed bioreactor operating in a continuous mode over an extended period of time. Throughput should be adjusted to control product concentration at {approximately}100 g/L (i.e., as close to the threshold of inhibition as possible). There appears to be no inherent design limitation to effect the engineering improvements required in the advanced process operation. The above scenario assumes that the presently available, product-inhibited organisms would be used. In a longer-term, more difficult research effort, it might be possible to reduce or eliminate product inhibition. As a result, price would be reduced further to $1.75 for the Zymomonas system or $1.85 for the yeast fermentation. It is recommended that the engineering proveout of the advanced process be continued at a pilot scale and that a laboratory program aimed at reducing product inhibition and/or increasing specific productivity be initiated. 49 refs., 11 figs., 19 tabs.

Busche, R.M. (Bio En-Gene-Er Associates, Inc., Wilmington, DE (United States)); Scott, C.D.; Davison, B.H. (Oak Ridge National Lab., TN (United States)); Lynd, L.R. (Dartmouth Coll., Hanover, NH (United States))

1991-08-01T23:59:59.000Z

336

Genome Sequence of Thermotoga sp Strain RQ2, a Hyperthermophilic Bacterium Isolated from a Geothermally Heated Region of the Seafloor near Ribeira Quente, the Azores  

SciTech Connect (OSTI)

Thermotoga sp. strain RQ2 is probably a strain of Thermotoga maritima. Its complete genome sequence allows for an examination of the extent and consequences of gene flow within Thermotoga species and strains. Thermotoga sp. RQ2 differs from T. maritima in its genes involved in myo-inositol metabolism. Its genome also encodes an apparent fructose phosphotransferase system (PTS) sugar transporter. This operon is also found in Thermotoga naphthophila strain RKU-10 but no other Thermotogales. These are the first reported PTS transporters in the Thermotogales.

Swithers, Kristen S [University of Connecticut, Storrs; DiPippo, Jonathan L [University of Connecticut, Storrs; Bruce, David [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Lykidis, A [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Stetter, Karl O [Universitat Regensburg, Regensburg, Germany; Nelson, Karen E [J. Craig Venter Institute; Gogarten, Peter [University of Connecticut, Storrs; Noll, Kenneth M [University of Connecticut, Storrs

2011-01-01T23:59:59.000Z

337

Cell, Vol. 121, 401409, May 6, 2005, Copyright 2005 by Elsevier Inc. DOI 10.1016/j.cell.2005.02.032 Developmental Commitment in a Bacterium  

E-Print Network [OSTI]

in which to at-Cambridge, Massachusetts 02138 2 Department of Microbiology tempt to identify genes, switches on an unusually large tion to become irreversible (Gilbert, 2000). As Hans regulon consisting

Symington, Lorraine S.

338

CO2 exposure at pressure impacts metabolism and stress responses in the model sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough  

SciTech Connect (OSTI)

Geologic carbon dioxide (CO2) sequestration drives physical and geochemical changes in deep subsurface environments that impact indigenous microbial activities. The combined effects of pressurized CO2 on a model sulfate-reducing microorganism, Desulfovibrio vulgaris, have been assessed using a suite of genomic and kinetic measurements. Novel high-pressure NMR time-series measurements using 13C-lactate were used to track D. vulgaris metabolism. We identified cessation of respiration at CO2 pressures of 10 bar, 25 bar, 50 bar, and 80 bar. Concurrent experiments using N2 as the pressurizing phase had no negative effect on microbial respiration, as inferred from reduction of sulfate to sulfide. Complementary pressurized batch incubations and fluorescence microscopy measurements supported NMR observations, and indicated that non-respiring cells were mostly viable at 50 bar CO2 for at least four hours, and at 80 bar CO2 for two hours. The fraction of dead cells increased rapidly after four hours at 80 bar CO2. Transcriptomic (RNA-Seq) measurements on mRNA transcripts from CO2-incubated biomass indicated that cells up-regulated the production of certain amino acids (leucine, isoleucine) following CO2 exposure at elevated pressures, likely as part of a general stress response. Evidence for other poorly understood stress responses were also identified within RNA-Seq data, suggesting that while pressurized CO2 severely limits the growth and respiration of D. vulgaris cells, biomass retains intact cell membranes at pressures up to 80 bar CO2. Together, these data show that geologic sequestration of CO2 may have significant impacts on rates of sulfate reduction in many deep subsurface environments where this metabolism is a key respiratory process.

Wilkins, Michael J.; Hoyt, David W.; Marshall, Matthew J.; Alderson, Paul A.; Plymale, Andrew E.; Markillie, Lye Meng; Tucker, Abigail E.; Walter, Eric D.; Linggi, Bryan E.; Dohnalkova, Alice; Taylor, Ronald C.

2014-09-01T23:59:59.000Z

339

Complete Genome Sequence of the Marine Cellulose- and Xylan-Degrading Bacterium Glaciecolasp. Strain 4H-3-7+YE-5  

Science Journals Connector (OSTI)

...This study was funded in part by the BioEnergy Science Center, a U.S. Department of Energy Bioenergy Research Centersupported by the Office...Psychrobacterin seawater collected off Ushuaia, Argentina, sub-Antarctica. FEMS Microbiol...

Barbara Klippel; Adriane Lochner; David C. Bruce; Karen Walston Davenport; Chris Detter; Lynne A. Goodwin; James Han; Shunsheng Han; Miriam L. Land; Natalia Mikhailova; Matt Nolan; Len Pennacchio; Sam Pitluck; Roxanne Tapia; Tanja Woyke; Sigrid Wiebusch; Alexander Basner; Fumiyoshi Abe; Koki Horikoshi; Martin Keller; Garabed Antranikian

2011-06-24T23:59:59.000Z

340

Dynamics of Metabolic Activities and Gene Expression in the Roseobacter Clade Bacterium Phaeobacter sp. Strain MED193 during Growth with Thiosulfate  

Science Journals Connector (OSTI)

...metabolic processes used to exploit the energy potentially gained from inorganic sulfur...lithotrophic potential to gain additional energy from sulfur oxidation for both increasing...organic carbon compounds for obtaining energy and cellular building blocks. Nevertheless...

Saraladevi Muthusamy; Federico Baltar; José M. González; Jarone Pinhassi

2014-08-29T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Dynamics of Metabolic Activities and Gene Expression in the Roseobacter Clade Bacterium Phaeobacter sp. Strain MED193 during Growth with Thiosulfate  

Science Journals Connector (OSTI)

...basis of the genome sequence. The SYBR green-based qRT-PCR was carried out by using...inhibitor [20 U/ml]), and 5 mul of 2 SYBR green master mix with 0.45 muM each primer...with these levels of growth stimulation, energy yields for thiosulfate oxidation calculated...

Saraladevi Muthusamy; Federico Baltar; José M. González; Jarone Pinhassi

2014-08-29T23:59:59.000Z

342

Assimilation and respiration of radioactive ethylene glycol, in the presence of high sodium chloride concentrations, by a sodium chloride requiring bacterium  

E-Print Network [OSTI]

containing 2 gm ethylene glycol and salts in 250 ml of distilled H20 which was started with a small inoculum of 2 ml suspension (11. 8 mg dry wt) removed 1. 96 gm glycol in 136 hr. Use of a nitrogen-limited medium allowed most of the ethylene glycol... growth on ethylene glycol or propylene glycol favored oxidation of ethylene glycol over glucose while prior growth on glucose favored oxidation of glucose over the iv glycols. Age of the culture had no dramatic effect on the rate of respiration...

Gonzalez, Carlos Francisco

2012-06-07T23:59:59.000Z

343

Isolation from the Sorghum bicolorMycorrhizosphere of a Bacterium Compatible with Arbuscular Mycorrhiza Development and Antagonistic towards Soilborne Fungal Pathogens  

Science Journals Connector (OSTI)

...surface sterilized as previously described (). Plants were grown in a sterilized clay loam soil-calcined clay (Oil Dry Type III; OILDRI, Limited, Wisbech, United Kingdom) mixture (1:1) in sterile Sunbags (catalog no. 7026; Sigma...

S. W. Budi; D. van Tuinen; G. Martinotti; S. Gianinazzi

1999-11-01T23:59:59.000Z

344

Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli  

Science Journals Connector (OSTI)

...ARTICLE PUBLIC HEALTH MICROBIOLOGY Does the Antibacterial Activity of...complete charge neutralization does not happen, whereas at a higher...P. R. Griffiths (ed.), Handbook of vibrational spectroscopy...Chem. Mat. 11: 545-546. Does the antibacterial activity of...

Sukdeb Pal; Yu Kyung Tak; Joon Myong Song

2007-01-19T23:59:59.000Z

345

Effects of nitrogen source and concentration upon glutamine synthetase and protease activity in the rumen bacterium Prevotella ruminicola strain B1 4  

E-Print Network [OSTI]

(mineral salts, essential volatile fatty acids, vitamin cofactors, 12 mM glucose, cysteine-HCL as a reductant and sodium carbonate) with variable amounts of ammonium chloride, pepticase or casein as the nitrogen source. The results indicated that when...

Kirk, James Michael

1995-01-01T23:59:59.000Z

346

Effect of Butylated Hydroxytoluene (BHT) on the Aerobic Biodegradation of a Model Vegetable Oil in Aquatic Media  

Science Journals Connector (OSTI)

Becker, P.; Koster, D.; Popov, M. N.; Markossian, S.; Antranikian, G.; Markl, H.The biodegradation of olive oil and the treatment of lipid-rich wool scouring wastewater under aerobic thermophilic conditions Water Resour. ... The biodegradation of olive oil and the treatment of lipid-rich wool scouring wastewater under aerobic thermophilic conditions ... The biodegrdn. of olive oil and the treatment of the lipid-rich wool scouring wastewater were studied under aerobic thermophilic (65°) conditions using Bacillus thermoleovorans IHI-91. ...

Darine A. Salam; Makram T. Suidan; Albert D. Venosa

2012-06-01T23:59:59.000Z

347

E-Print Network 3.0 - anaerobic continuously stirred Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

or thermophilic... ;12;Abstract Anaerobic digestion of dairy manure produces biogas that can be captured and used for fuel while... offering environmental benefits. Dairy...

348

E-Print Network 3.0 - altered dna polymerase Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

University in St. Louis Collection: Biology and Medicine 9 Very Efficient TemplatePrimer-Independent DNA Synthesis by Thermophilic DNA Polymerase in the Presence of a...

349

E-Print Network 3.0 - amycolatopsis sp strain Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

thermotolerant methylotrophic Bacillus strains involving a novel catabolic NAD-dependent methanol dehydrogenase... and characterization of a thermophilic sulfate-reducing...

350

Analysis of Bacterial Detachment from Substratum Surfaces by the Passage of Air-Liquid Interfaces  

Science Journals Connector (OSTI)

...collision efficiency, EA is the bubble-bacterium attachment efficiency, and ES is the stability of the bubble-bacterium aggregate. The...determines the bacterium-bubble aggregate stability (). For the dimensions considered...

Cristina Gómez-Suárez; Henk J. Busscher; Henny C. van der Mei

2001-06-01T23:59:59.000Z

351

Bioreduction of hematite nanoparticles by the dissimilatory iron...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

nanoparticles by the dissimilatory iron reducing bacterium Shewanella oneidensis MR-1. Bioreduction of hematite nanoparticles by the dissimilatory iron reducing bacterium...

352

Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 Å resolution  

Science Journals Connector (OSTI)

The three-dimensional structure of the uncomplexed 20K endoglucanase from the thermophilic fungus M. albomyces is mostly similar to that of previously determined family 45 endoglucanases, except that the flexible loop between strands V and VI is well ordered. Thermophilic and mesophilic endoglucanases of this family can now be compared.

Valjakka, J.

2003-03-25T23:59:59.000Z

353

Complete Genome Sequence of the Anaerobic Halophilic Alkalithermophile Natranaerobius thermophilus JW/NM-WN-LFT  

SciTech Connect (OSTI)

The genome of the anaerobic halophilic alkalithermophile Natranaerobius thermophiles consists of one chromosome and two plasmids.The present study is the first to report the completely sequenced genome of polyextremophile and the harboring genes harboring genes associated with roles in regulation of intracellular osmotic pressure, pH homeostasis, and thermophilic stability.

Mesbah, Noha [University of Georgia, Athens, GA; Dalin, Eileen [U.S. Department of Energy, Joint Genome Institute; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Larimer, Frank W [ORNL; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Wiegel, Juergen [University of Georgia, Athens, GA

2011-01-01T23:59:59.000Z

354

A Bioreactor for Growth of Sulfate-Reducing Bacteria: Online Estimation of Specific Growth Rate and Biomass for the Deep-Sea  

E-Print Network [OSTI]

of Specific Growth Rate and Biomass for the Deep-Sea Hydrothermal Vent Thermophile Thermodesulfatator indicus, a chemolithotrophic, thermophilic, sulfate-reducing bac- terium recently isolated from a deep-sea hydrothermal vent donor and CO2 as primary carbon source. These experiments were designed to measure growth kinetics under

Reysenbach, Anna-Louise

355

Three-dimensional structure of the surface protein of Desulfurococcus mobilis.  

Science Journals Connector (OSTI)

...Desulfurococcus mobilis. | The spherical cells of the thermophilic...14 September 1987 The spherical cells of the thermophilic...The bacteria appear spherical in shape, with a diameter...were carried out with crystal fragments exhibiting...since they are somewhat bent near their ends, they...

I Wildhaber; U Santarius; W Baumeister

1987-12-01T23:59:59.000Z

356

Chapter 7 - Consolidated Bioprocessing for Ethanol Production  

Science Journals Connector (OSTI)

Abstract Ethanol production from cellulosic biomass involves five unit operations: pretreatment, cellulase production, enzymatic hydrolysis, microbial fermentation, and product recovery. ­Consolidated bioprocessing (CBP) combines the three biologically mediated steps (cellulase production, enzymatic hydrolysis, and microbial fermentation) into a single operation. CBP has outstanding potential for providing a breakthrough solution for the biological conversion of cellulosic biomass into ethanol. The implementation of CBP requires microbes that can produce a functional cellulase system while generating ethanol at high yields and concentrations. CBP-enabling microorganisms can be developed via two strategies: a native cellulolytic strategy, which involves identifying a naturally occurring cellulolytic microorganism (or a consortium of microorganisms) and then improving its ability to ferment sugars into ethanol at high yields and at high titers, and a recombinant cellulolytic strategy, which involves engineering noncellulolytic organisms so that they can utilize cellulose to produce ethanol at high yields and titers by heterologously expressing cellulases.

Zhiliang Fan

2014-01-01T23:59:59.000Z

358

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity  

Science Journals Connector (OSTI)

...S91 and Zoospores of the Green Alga Ulva australis Independent of...S91 and zoospores of the green alga Ulva australis. The antiadhesive...drag and thereby leads to high fuel consumption (40) and CO2 emissions...furanones produced by the red algae Delisea pulchra (8), but...

Nete Bernbom; Yoke Yin Ng; Staffan Kjelleberg; Tilmann Harder; Lone Gram

2011-10-14T23:59:59.000Z

359

Epidemiologic profile of Staphylococcus aureus-methicilin resistant (MRSA) bacterium in hospital-acquired-infection in neonatal-intensive-care-unit (ICU) from -2000 to 2010 analysis in general hospital  

Science Journals Connector (OSTI)

Awareness of epidemiologic profile of MRSA-hospital-acquired infection in neonatal-ICU might improve...

LF Baqueiro-Freitas; CI Santos; F Ferreira

2011-06-01T23:59:59.000Z

360

Recoding of the stop codon UGA to glycine by a BD1-5/SN-2 bacterium and niche partitioning between Alpha- and Gammaproteobacteria in a tidal sediment microbial community naturally selected in a laboratory chemostat  

SciTech Connect (OSTI)

Sandy coastal sediments are global hot spots for microbial mineralization of organic matter and denitrification. These sediments are characterized by advective pore water flow, tidal cycling and an active and complex microbial community. Metagenomic sequencing of microbial communities sampled from such sediments showed that potential sulfuroxidizing Gammaproteobacteria and members of the enigmaticBD1-5/ SN-2 candidatephylumwereabundantinsitu (>10% and 2% respectively). By mimicking the dynamic oxic/anoxic environmental conditions of the sedimentin a laboratory chemostat, a simplified microbial community was selected from the more complex inoculum. Metagenomics, proteomics and fluorescenceinsituhybridization showed that this simplified community contained both a potential sulfuroxidizing Gamma proteobacteria (at 24 2% abundance) and a member of the BD1-5 / SN-2candidatephylum (at 7 6%abundance). Despite the abundant supply of organic substrates to the chemostat, proteomic analysis suggested that the selected gamma proteobacterium grew partially auto trophically and performed hydrogen/formate oxidation. The enrichment of a member of the BD1-5/SN-2candidatephylum enabled, for the first time, direct microscopic observation by fluorescent insitu hybridization and the experimental validation of the previously predicted translation of the stop codon UGA into glycine.

Hanke, Anna [Max Planck Institute for Marine Microbiology; Hamann, Emmo [Max Planck Institute for Marine Microbiology; Sharma, Ritin [ORNL; Geelhoed, Jeanine [Max Planck Institute for Marine Microbiology; Hargesheimer, Theresa [Max Planck Institute for Marine Microbiology; Kraft, Beate [Max Planck Institute for Marine Microbiology; Meyer, Volker [Max Planck Institute for Marine Microbiology; Lenk, Sabine [Max Planck Institute for Marine Microbiology; Osmers, Harald [Max Planck Institute for Marine Microbiology; Wu, Rong [Delft University of Technology, Delft, Netherlands; Makinwa, Kofi [Delft University of Technology, Delft, Netherlands; Hettich, Robert {Bob} L [ORNL; Banfield, Jillian F. [University of California, Berkeley; Tegetmeyer, Halina [Max Planck Institute for Marine Microbiology; Strouss, Marc [University of Calgary, ALberta, Canada

2014-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

E-Print Network 3.0 - alkaliphilic bacillus sp Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Zhang,J., Zhang,Z., Miller,W., and Lipman,D.J. (1997) Summary: in a recombinant alpha-amylase of thermophilic and alkaliphilic Bacillus sp. strain TS-23. Extremophiles 7:...

362

Characterization and molecular cloning of thermostable alpha-amylase from Streptomyces sp.To1  

Science Journals Connector (OSTI)

A new thermophilic Streptomyces...sp. TO1, isolated from Tunisian soil, produced a thermostable alpha-amylase and pullulanase. The gene encoding for the alpha-amylase activity was cloned into the multicopy clonin...

Lotfi Mellouli; Raoudha Ghorbel; Alya Kammoun; Monia Mezghani…

1996-07-01T23:59:59.000Z

363

E-Print Network 3.0 - alkaliphilic bacillus isolate Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Zhang,J., Zhang,Z., Miller,W., and Lipman,D.J. (1997) Summary: in a recombinant alpha-amylase of thermophilic and alkaliphilic Bacillus sp. strain TS-23. Extremophiles 7:...

364

E-Print Network 3.0 - anaerobic xylose fermentation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

(Glucose... ) Thermophilic Fermentation (Xylose) Wet oxidation 196C, 12 Bar O2 NaCO2 Biogas Ethanol 55C 32C 70C 12... or transport 6.4 Ris Energy Report 2 Introduction...

365

E-Print Network 3.0 - anaerobic ethanol oxidation Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

) Thermophilic Fermentation (Xylose) Wet oxidation 196C, 12 Bar O2 NaCO2 Biogas Ethanol 55C 32C 70C 12... 90% of new cars have engines specially designed to...

366

E-Print Network 3.0 - archaea dominate sedimentary Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and compressed into rock. Metamorphic rock: sedimentary rock that has... of Life The LCA may be a Thermophile 12;The Tree of Life 12;The Archaea Archaea often inhabit...

367

Isolation of a Novel Human Gene, APCDD1, as a Direct Target of the ?-Catenin/T-Cell Factor 4 Complex with Probable Involvement in Colorectal Carcinogenesis  

Science Journals Connector (OSTI)

...Cummings N., Harris G. W., Debeire P., Breton C. A single domain thermophilic xylanase can bind insoluble xylan: evidence for surface aromatic clusters. Biochim. Biophys. Acta, 1433: 110-121, 1999. 21 Liu W., Dong X., Mai M., Seelan...

Meiko Takahashi; Manabu Fujita; Yoichi Furukawa; Ryuji Hamamoto; Takashi Shimokawa; Nobutomo Miwa; Michio Ogawa; and Yusuke Nakamura

2002-10-15T23:59:59.000Z

368

Isolation of a Novel Human Gene, APCDD1, as a Direct Target of the ?-Catenin/T-Cell Factor 4 Complex with Probable Involvement in Colorectal Carcinogenesis  

Science Journals Connector (OSTI)

...development of novel strategies for diagnosis, treatment, and prevention of colorectal cancers. Materials...domain thermophilic xylanase can bind insoluble xylan: evidence for surface aromatic clusters. Biochim. Biophys. Acta, 1433...

Meiko Takahashi; Manabu Fujita; Yoichi Furukawa; Ryuji Hamamoto; Takashi Shimokawa; Nobutomo Miwa; Michio Ogawa; and Yusuke Nakamura

2002-10-15T23:59:59.000Z

369

The use of single tryptophan variants to study protein folding and stability  

E-Print Network [OSTI]

Studies on the kinetics of protein folding of the histidine-containing phosphocarrier protein (HPr) from the thermophile Bacillus stearothermophilus (Bst) will contribute much to the understanding of the origins of its enhanced thermal stability...

Dulin, Jennifer Natalie

2013-02-22T23:59:59.000Z

370

proteinsSTRUCTURE O FUNCTION O BIOINFORMATICS Hydrophobic environment is a key factor for  

E-Print Network [OSTI]

proteinsSTRUCTURE O FUNCTION O BIOINFORMATICS Hydrophobic environment is a key factor, Georgia 30332 INTRODUCTION Elucidating the molecular mechanisms responsible for the stability of thermophilic proteins is an important task for engineering stable proteins. Enhancing protein stability

Gaucher, Eric

371

Ethanol fermentation from molasses at high temperature by thermotolerant yeast Kluyveromyces sp. IIPE453 and energy assessment for recovery  

Science Journals Connector (OSTI)

High temperature ethanol fermentation from sugarcane molasses B using thermophilic...Kluyveromyces...sp. IIPE453 was carried out in batch bioreactor system. Strain was found to have a maximum specific ethanol pr...

Diptarka Dasgupta; Prasenjit Ghosh…

2014-10-01T23:59:59.000Z

372

Altered Fermentative Metabolism in Chlamydomonas reinhardtii Mutants Lacking Pyruvate Formate Lyase and Both Pyruvate Formate Lyase and Alcohol Dehydrogenase  

Science Journals Connector (OSTI)

...was quantified by qRT-PCR using the Engine Opticon system (Bio-Rad). First-strand...A Laboratory Manual (New York: Cold Spring Harbor Laboratory Press). Shimogawara...thermophilic cyanobacteria inhabiting hot spring microbial mats. Proc. Natl. Acad...

Claudia Catalanotti; Alexandra Dubini; Venkataramanan Subramanian; Wenqiang Yang; Leonardo Magneschi; Florence Mus; Michael Seibert; Matthew C. Posewitz; Arthur R. Grossman

2012-02-21T23:59:59.000Z

373

Characterization of the Allosteric Properties of Thermus thermophilus Phosphofructokinase and the Sources of Strong Inhibitor Binding Affinity and Weak Inhibitory Response  

E-Print Network [OSTI]

Characterization of allosteric properties of phosphofructokinase from the extreme thermophile Thermus thermophilus (TtPFK) using thermodynamic linkage analysis revealed several peculiarities. Inhibition and activation of Fru-6-P binding...

Shubina-McGresham, Maria

2012-10-19T23:59:59.000Z

374

A comparative study of HPr proteins from extremophilic organisms  

E-Print Network [OSTI]

of the proteins were derived from moderate thermophiles (Streptococcus thermophilus and Bacillus staerothermophilus) and two from haloalkaliphilic organisms (Bacillus halodurans and Oceanobacillus iheyensis); these proteins were compared with HPr from...

Syed Ali, Abbas Razvi

2006-04-12T23:59:59.000Z

375

Effects of Temperature on Aerobic Treatment of Anaerobically Digested Palm Oil Mill Effluent (POME)  

Science Journals Connector (OSTI)

wool scouring ww ... The TCOD removal efficiencies at thermophilic conditions fall in an acceptable range between 72?80% although the values were lower than the TCOD removal efficiency at mesophilic conditions (95%). ... Figure 4 shows that the efficiency of BOD removal decreased when the temperature increased from mesophilic to thermophilic range; with an operating temperature of 28 °C (97% BOD removal) being the most efficient while the least efficient was at 55 °C (76% BOD removal). ...

Yi Jing Chan; Mei Fong Chong; Chung Lim Law

2010-07-02T23:59:59.000Z

376

Differences in Haemophilus parasuis adherence to and invasion of AOC-45 porcine aorta endothelial cells  

Science Journals Connector (OSTI)

The pathogenesis of Haemophilus parasuis depends on the bacterium’s ability to interact with endothelial cells and invade adjacent tissues. In this study, we investigated the abilities of eight H. parasuis...refe...

Rafael Frandoloso; Mateus Pivato; Sonia Martínez-Martínez…

2013-10-01T23:59:59.000Z

377

Optimized procedures for extractioin, purification and characterization of exopolymeric substances (eps) from two bacteria (sagittula stellata and pseudomonas fluorescens biovar ii) with relevance to the study of actinide binding in aquatic environments  

E-Print Network [OSTI]

The extracellular polymeric substances (EPS) of marine bacterium Sagittula stellata and soil bacterium Pseudomonas fluorescens Biovar II, were extracted by six methods referred to the bibliography, efficacies of which were compared based on the EPS...

Xu, Chen

2009-05-15T23:59:59.000Z

378

Dispersant solutions for dispersing hydrocarbons  

DOE Patents [OSTI]

A dispersant solution includes a hydrocarbon dispersing solution derived from a bacterium from ATCC 75527, ATCC 75529, or ATCC 55638.

Tyndall, R.L.

1997-03-11T23:59:59.000Z

379

Download  

Science Journals Connector (OSTI)

1986. Pigment organization and energy transfer in the green photosynthetic bacterium. Chloroflexus aurantiacus. 1. The cytoplasmic membrane. Photosynth.

2000-03-18T23:59:59.000Z

380

Volatile fatty acid fermentation of AFEX-treated newspaper and bagasse by rumen microorganisms  

E-Print Network [OSTI]

) of cellulolytic bacteria attack the cellulosic materials and hydrolyze them to simple sugars; (ii) the sugars are rapidly fermented largely to VFA's and carbon dioxide, and (iii) methane is formed by slow-growing methanogenic bacteria Anaerobic fermentation..., and on the different acid molar percents. AFEX-treated and non-treated bagasse and newspaper were ground and fermented by a mixed population of rumen microorganisms to produce VFA's and carbon dioxide. The fermentation process was similar to that which occurs...

Blasig, Jorge Dari?o

1991-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

fulltext.pdf  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

I: I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS 2 David Muddiman & Genna Andrews & Derrick Lewis & Jaspreet Notey & Robert Kelly Received: 3 May 2010 / Revised: 13 June 2010 / Accepted: 20 June 2010 / Published online: 10 July 2010 # Springer-Verlag 2010 Abstract The proteome of extremely thermophilic micro- organisms affords a glimpse into the dynamics of microbial ecology of high temperature environments. The secretome, or extracellular proteome of these microorganisms, no doubt harbors technologically important enzymes and other ther- mostable biomolecules that, to date, have been characterized only to a limited extent. In the first of a two-part study on selected thermophiles, defining the secretome requires a sample preparation method that has no negative impact on all downstream

382

AEM.01400-10v1.pdf  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Oct 8, 2010 rmk Oct 8, 2010 rmk Phylogenetic, microbiological and glycoside hydrolase diversity within the extremely thermophilic, plant biomass-degrading genus Caldicellulosiruptor 5 Sara E. Blumer-Schuette, Derrick L. Lewis # , and Robert M. Kelly * Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905 # Present address: Novozymes Biologicals, Salem, VA 10 Submitted to: Applied and Environmental Microbiology (June, 2010) Running title: Plant biomass deconstruction by Caldicellulosiruptor species 15 Keywords: Caldicellulosiruptor, extreme thermophile, plant biomass, glycoside hydrolases *Address correspondence to: Robert M. Kelly 20 Department of Chemical and Biomolecular Engineering

383

Licensing : BioEnergy Science Center  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Inventions Inventions 32 records A Broad Environmental Stress-Inducible Promoter and its Application in Crops A Novel Monolignol that reduces recalcitrance of plant cell walls Caloramator sp. Tolerance of Pretreatment Inhibitors from LIgnocellulosics Cellulose and xylan fermentation by novel anaerobic thermophilic clostridia isolated from self-heated biocompost Compositions and Methods for Improved Plant Feedstock Consolidated Bioprocessing Method using Thermophilic Microorganisms Engineering male sterility or non-transgenic pollen by pollen-specific expression of a restriction enzyme Flow-through Pretreatment of Lignocellulosic Biomass with Inorganic Nanoporous Membranes Gene and Gene Clusters that Enable Degradation of Recalcitrant Biological Materials Genes to Increase Growth in Monocots

384

Why Sequence Thermovibrio ammonificans?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Thermovibrio ammonificans? Thermovibrio ammonificans? Thermophilic and hyperthermophilic microorganisms are an important component of geothermal ecosystems, which include continental hot springs, solfataras, and both shallow and deep-sea hydrothermal vents. These organisms play a critical role in our understanding of several fundamental biological processes, such as microbial adaptations to elevated temperatures and prokaryotic evolution, and they represent a natural source for the discovery of thermostable enzymes. T. ammonificans in the process of dividing. Photo courtesy Valentin Starovoytov and Costantino Vetriani, Rutgers University The isolation and characterization of microorganisms that are thermophilic, anaerobic, and chemolithoautotrophic (getting energy by oxidizing inorganic

385

Influence of Methylation on the Antibacterial Properties of Triclosan in Pasteurella multocida and Pseudomonas aeruginosa Variant Strains.  

E-Print Network [OSTI]

??The opportunistic bacterium Pasteurella multocida is extremely susceptible to the hydrophobic biocide triclosan by virtue of its markedly permeable outer membrane, while the opportunistic pathogen… (more)

Clayborn, Ashton B.

2011-01-01T23:59:59.000Z

386

E-Print Network 3.0 - agents causing diseases Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

disease is an inflammatory disease caused by a bacterium transmitted by ticks (small bloodsucking... that scientists think may transmit Ebola, a usually fatal ... Source:...

387

E-Print Network 3.0 - aureus cc398 infections Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

isolate with a DNA sequence >95% identical to the Staphylococcus aureus... have serious implications in public health 12. When a phage infects a bacterium, two alternative...

388

E-Print Network 3.0 - aureus musculoskeletal infections Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

isolate with a DNA sequence >95% identical to the Staphylococcus aureus... have serious implications in public health 12. When a phage infects a bacterium, two alternative...

389

Download  

Science Journals Connector (OSTI)

The bacterium converts hydrogen sulfide to clemental sulfur which is then ..... element in the biological economy of the area. Evidence has been presented ...

390

Purification of soluble and active RaxH, a transmembrane histidine protein kinase from Xanthomonas oryzae pv. oryzae required for AvrXa21 activity.  

E-Print Network [OSTI]

of the plant pathogenic bacterium Xanthomonas campestris pv.oryzae pv. oryzae isolates in transgenic plants. Mol. Plant–Xanthomonas oryzae pv. oryzae. Mol. Plant–Microbe Interact.

Stolov, Avital; Valverde, Angel; Ronald, Pamela; Burdman, Saul

2007-01-01T23:59:59.000Z

391

Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: Global transcriptomic and proteomic analyses  

E-Print Network [OSTI]

2005). Genome sequence of the PCE-dechlorinating bacteriumHong Kong. chloroethene (PCE) and trichloroethene (TCE) toexperimental co-culture, PCE was dechlorinated to vinyl

Men, Y.

2012-01-01T23:59:59.000Z

392

Analysis of Genes Involved in Anaerobic Growth in Porphyromonas Gingivalis and Shewanella Oneidensis MR-1.  

E-Print Network [OSTI]

?? Porphyromonas gingivalis is an oral Gram-negative anaerobic bacterium implicated in periodontal disease, a polymicrobial inflammatory disease that is correlated with cardiovascular disease, diabetes and… (more)

Kumarasinghe, Dilini Sanjeevi

2013-01-01T23:59:59.000Z

393

1  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

companies and researchers to see the detailed inner workings of molecular machines. "Inside each cell in our bodies and inside every bacterium and virus are tiny but...

394

Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae  

SciTech Connect (OSTI)

Vibrio cholerae, the causative agent of cholera, is a bacterium autochthonous to the aquatic environment, and a serious public health threat.

Chun, Jongsik; Grim, Christopher J.; Hasan, Nur A.; Lee, Je H.; Choi, Seon Y.; Haley, Bradd J.; Taviani, Elisa; Jeon, Yoon-Seong; Kim, Dong W.; Lee, Jae-Hak; Brettin, T.; Bruce, David; Challacombe, Jean; Detter, J. C.; Han, Cliff F.; Munk, A. C.; Chertkov, Olga; Meincke, Linda; Saunders, Elizabeth; Walters, Ronald A.; Huq, Anwar; Nair, G. B.; Colwell, Rita R.

2009-09-08T23:59:59.000Z

395

RNA Type III Secretion Signals that require Hfq. | EMSL  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that require Hfq. RNA Type III Secretion Signals that require Hfq. Abstract: effector proteins from the bacterium to a host cell; however, the secretion signal is poorly...

396

E-Print Network 3.0 - antimicrobial susceptibilities phage Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of infection), by increasing exposure to susceptible hosts (Rhodes & Anderson 1996; Boots & Sasaki 1999... plant bacterium Pseudomonas fluorescens strain SBW25 (Rainey & Bailey...

397

Fermilab Today - Safety Tip of the Week Archive  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

out a student loan in my name." September 28, 2009 Childhood vaccination for whooping cough wears off Whooping cough, caused by bordetella pertussis bacterium, was once thought...

398

Linearly concatenated cyclobutane lipids form a dense bacterial membrane  

Science Journals Connector (OSTI)

... K. et al. Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate. Arch. Microbiol. 175, 198–207 (2001)

Jaap S. Sinninghe Damsté; Marc Strous; W. Irene C. Rijpstra; Ellen C. Hopmans; Jan A. J. Geenevasen; Adri C. T. van Duin; Laura A. van Niftrik; Mike S. M. Jetten

2002-10-17T23:59:59.000Z

399

Energetics of Bacterial Photosynthesis  

Science Journals Connector (OSTI)

Energetics of Bacterial Photosynthesis ... We report the results of extensive numerical simulations and theoretical calculations of electronic transitions in the reaction center of Rhodobacter sphaeroides photosynthetic bacterium. ...

David N. LeBard; Dmitry V. Matyushov

2009-08-19T23:59:59.000Z

400

RESEARCH ARTICLE Open Access Differences in lateral gene transfer in hypersaline  

E-Print Network [OSTI]

-domain LGT events are not uncommon. These distant transfer events offer potentially greater fitness. Results: We identified potential inter-class LGT events into both a thermophilic class of Archaea (Thermoprotei) and a halophilic class of Archaea (Halobacteria). We then categorized these LGT genes

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Geobiology (2003), 1, 119127 2003 Blackwell Publishing Ltd 119  

E-Print Network [OSTI]

) to the thermophilic hydrogen-oxidizing Hydrogenobacter thermolithotrophum, and is the first report of Hydrogenobacter Ridge, Isu­Ogasawara Arc, Okinawa Trough and the Manus Basin. A single sequence was closely related producers are chemolithoautotrophic micro-organisms that utilize a wide range of available redox couples

Reysenbach, Anna-Louise

402

Anaerobic methane oxidation in metalliferous hydrothermal sediments: influence on carbon flux and  

E-Print Network [OSTI]

Anaerobic methane oxidation in metalliferous hydrothermal sediments: influence on carbon flux significant sink that regulates methane flux from sediments into the oceans and atmosphere. Here we examine mesophilic to thermophilic AOM in hydro- thermal sediments recovered from the Middle Valley vent field

Girguis, Peter R.

403

Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Strain  

Science Journals Connector (OSTI)

...was prefiltered through glass wool to remove residual undegraded...nature of the resistance of wool to digestion by enzymes. Bur...production of shrinkage resistant wool and mixture fabrics. J. Soc...in thermophilic poultry waste digesters and enrichment of a feather-degrading...

Xiang Lin; Chung-Ginn Lee; Ellen S. Casale; Jason C. H. Shih

1992-10-01T23:59:59.000Z

404

High-resolution single-molecule characterization of the enzymatic states in Escherichia coli F1-ATPase  

Science Journals Connector (OSTI)

...the enzymatic states in Escherichia coli F1-ATPase Thomas Bilyard 1 Mayumi Nakanishi-Matsui...individual molecules of life . The rotary motor F1-ATPase from the thermophilic Bacillus...best-studied of all molecular machines. F1-ATPase is the part of the enzyme F1FO-ATP...

2013-01-01T23:59:59.000Z

405

In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sludges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I  

Science Journals Connector (OSTI)

...cloning-based analysis revealed that the...other thermophilic reactor that had been treating...16S rDNA-cloning analysis combined with fluorescent...difficulty or lower reliability in accomplishing...sludge in a UASB reactor treating an artificial...community structure analysis was that unidentifiable...

Yuji Sekiguchi; Hiroki Takahashi; Yoichi Kamagata; Akiyoshi Ohashi; Hideki Harada

2001-12-01T23:59:59.000Z

406

Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity, or substrates.  

Science Journals Connector (OSTI)

...precursors are potential liquid fuel additives, industrial solvents...that washout from the culture vessel does not occur as readily as...have also shown that butyrate consumption in a thermophilic coculture...cessing municipal wastes for fuel and chemicals. Argonne National...

S E Lowe; M K Jain; J G Zeikus

1993-06-01T23:59:59.000Z

407

Eos, Vol. 87, No. 4, 24 January 2006 Geophysics program and the NOAA Ocean Ex-  

E-Print Network [OSTI]

: Relationships between hydrothermal and volcanic processes,Geophys.Res.Lett.,22(2),143­146. Holden.A.Feely (1987), Cataclysmic hydrothermal venting on the Juan de Fuca Ridge,Nature,329, 149­151. Bohnenstiehl,D,J.F.,M.Summit,and J.A.Baross (1998), Thermophilic and hyperthermophilic microorgan- isms in 3­30°C hydrothermal fluids

408

References 170 RREEFFEERREENNCCEESS  

E-Print Network [OSTI]

, Comparison Between Dynamics and Control Performance of Mesophilic and Thermophilic Anaerobic Sludge Digesters Controller for Distillation Columns in the Presence of Strong Directionality and Model Errors, Industrial Reactor Case Study, Industrial and Engineering Chemistry Research, 29: 1218-1226 Coughauowr, D.R., 1991

Skogestad, Sigurd

409

proteinsSTRUCTURE O FUNCTION O BIOINFORMATICS PROTS: A fragment based protein  

E-Print Network [OSTI]

which live at elevated temperatures as high as 1138C.5 Thus, the proteins produced by thermophiles and practically.1­8 Protein-based drugs have become increasingly attractive because of their high efficiency at higher temperature, which can lead to more efficient industrial processes because chemical reactions

Zhang, Yang

410

 

Science Journals Connector (OSTI)

...the obligate thermophilic flora and its economic importance such as is obtained by the...will not hold for molds which have the power to grow from one droplet to the other...e., an increase of the bactericidal power of the smoke, as well as visibly, a...

1928-01-01T23:59:59.000Z

411

Long term biosustainability in a high energy, low diversity crustal biome  

SciTech Connect (OSTI)

Geochemical, microbiological, and molecular analyses of alkaline saline groundwater at 2.8 kilometers depth in Archaean metabasalt revealed a microbial biome dominated by a single phylotype affiliated with thermophilic sulfate reducers belonging to Firmicutes. These sulfate reducers were sustained by geologically produced sulfate and hydrogen at concentrations sufficient to maintain activities for millions of years with no apparent reliance on photosynthetically derived substrates.

Lin, L-H.; Wang, P-L.; Rumble, D.; Lippmann-Pipke, J.; SherwoodLollar, B.; Boice, E.; Pratt, L.; Brodie, E.; Hazen, T.C.; Andersen,G.L.; DeSantis, T.; Moser, D.P.; Kershaw, D.; Onstott, T.

2006-10-01T23:59:59.000Z

412

Microbial Diversity of Hydrothermal Sediments in the Guaymas Basin: Evidence for Anaerobic Methanotrophic Communities  

Science Journals Connector (OSTI)

...hydrates in the Gulf of Mexico (46) and at cold methane...nonhydrothermal marine sediments as well as methane seeps (59...Desulfobulbaceae) as well as hyperthermophilic...closest relatives in geothermal springs, subsurface...with thermophilic as well as nonthermophilic representatives...

Andreas Teske; Kai-Uwe Hinrichs; Virginia Edgcomb; Alvin de Vera Gomez; David Kysela; Sean P. Sylva; Mitchell L. Sogin; Holger W. Jannasch

2002-04-01T23:59:59.000Z

413

Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a Thermus Isolate  

Science Journals Connector (OSTI)

...thermophilic anaerobic enrichment cultures from geothermal areas. . Urone P. F. Stability of colorimetric...investigators from a thermal spring in New Mexico. Strain NMX2 A.1 was also able to reduce...or S. | Department of Biology, New Mexico Institute of Mining and Technology, Socorro...

T. L. Kieft; J. K. Fredrickson; T. C. Onstott; Y. A. Gorby; H. M. Kostandarithes; T. J. Bailey; D. W. Kennedy; S. W. Li; A. E. Plymale; C. M. Spadoni; M. S. Gray

1999-03-01T23:59:59.000Z

414

Engineering rotor ring stoichiometries in the ATP synthase  

Science Journals Connector (OSTI)

...Limitations of gas-phase quantum mechanics...the University of Technology...Scientific Research and Development GmbH and Eurofins...10 Genetic fusion technology (3, 4) Thermophilic...10 Genetic fusion technology...Proton-powered turbine of a plant motor...

Denys Pogoryelov; Adriana L. Klyszejko; Ganna O. Krasnoselska; Eva-Maria Heller; Vanessa Leone; Julian D. Langer; Janet Vonck; Daniel J. Müller; José D. Faraldo-Gómez; Thomas Meier

2012-01-01T23:59:59.000Z

415

Anaerobic Coculture of Microalgae with Thermosipho globiformans and Methanocaldococcus jannaschii at 68°C Enhances Generation of n-Alkane-Rich Biofuels after Pyrolysis  

Science Journals Connector (OSTI)

...Tsukuba-Shi, Ibaraki, Japan We tested different alga-bacterium-archaeon consortia to investigate...subsurface oil deposit. We then tested various alga-bacterium-archaeon consortia to determine...unicellular, calcifying coccolithophorid alga of the phylum Haptophyta that is widely...

Kunio Yamane; Shigeru Matsuyama; Kensuke Igarashi; Motoo Utsumi; Yoshihiro Shiraiwa; Tomohiko Kuwabara

2012-11-26T23:59:59.000Z

416

Cloning, Expression, and Cell Surface Localization of Paenibacillus sp. Strain W-61 Xylanase 5, a Multidomain Xylanase  

Science Journals Connector (OSTI)

...no substantial xylan-binding activity. Cell surface localization of...Xyn5 to the cell surface of the bacterium...neighboring b-1,4-xylans by Xyn5 and the...expression, and cell surface localization of...have shown that a xylan-degrading bacterium...

Yasuko Ito; Toshio Tomita; Narayan Roy; Akito Nakano; Noriko Sugawara-Tomita; Seiji Watanabe; Naoko Okai; Naoki Abe; Yoshiyuki Kamio

2003-12-01T23:59:59.000Z

417

Processing of cellulosic material by a cellulase-containing cell-free fermentate produced from cellulase-producing bacteria, ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate, have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase degrading bacterium ATCC 55702, which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic materials. 5 figs.

Dees, H.C.

1998-08-04T23:59:59.000Z

418

Adenosine Triphosphate Pools in Methanobacterium  

Science Journals Connector (OSTI)

...adenine nucleotide pools and methane production...total nucleotide pool levels after 7 hr...shaken at 37 C in a water bath. Sym- bols...mole, the energy conservation by the bacterium...transferred to methane and water]. The bacterium...might affect ATP pool levels in whole cells...

A. M. Roberton; R. S. Wolfe

1970-04-01T23:59:59.000Z

419

Molecular organization in the native state of woody tissue: Studies of tertiary structure using the Raman microprobe solid state 13C NMR and biomimetic tertiary aggregates. Progress report, July 1, 1989--June 30, 1993  

SciTech Connect (OSTI)

We have previously shown that all relatively pure plant and bacterial celluloses are, in their native states, composites of two lattice forms, I{sub {alpha}} and I{sub {beta}}, and that the two forms possess molecular chains in the same conformations but with different hydrogen bonding patterns. In the current period we have demonstrated that in higher plant cell wall matrices, the hemicelluloses are likely to have a regulatory function during the aggregation of cellulose. Different hemicelluloses appear to influence the aggregation in different ways. We have also developed preliminary evidence indicating the hemicelluloses may have a protective function against the action of some cellulolytic enzymes. The specific accomplishments during the current period are detailed. Demonstration that hemicelluloses present during biogenesis can transform bacterial cellulose into a cellulose typical of higher plant celluloses, and that each hemicellulose has a different effect on the pattern of aggregation. Evidence is presented that the hemicelluloses may limit the action of certain cellulolytic enzymes, suggesting that their function may go beyond regulation to include passive resistance to cellulolytic pathogens. Enhancing the potential of the Raman microprobe technique for mapping variability of lignin in the cell wall by identifying the contribution of the different substructures of lignin to the intensity of the key band in the Raman spectrum of lignin. Mapping of the variability of lignin across two cell wall sections. The mappings have convoluted within them both composition and concentration, but they demonstrate the potential of the method and point to the improvements we are now making so as to distinguish between variability of concentration and variability of composition.

Atalla, R.H.

1993-12-31T23:59:59.000Z

420

Synergistic Enhancement of Cellobiohydrolase Performance on Pretreated Corn Stover by Addition of Xylanase and Esterase Activities  

SciTech Connect (OSTI)

Significant increases in the depolymerization of corn stover cellulose by cellobiohydrolase I (Cel7A) from Trichoderma reesei were observed using small quantities of non-cellulolytic cell wall-degrading enzymes. Purified endoxylanase (XynA), ferulic acid esterase (FaeA), and acetyl xylan esterase (Axe1) all enhanced Cel7A performance on corn stover subjected to hot water pretreatment. In all cases, the addition of these activities improved the effectiveness of the enzymatic hydrolysis in terms of the quantity of cellulose converted per milligram of total protein. Improvement in cellobiose release by the addition of the non-cellulolytic enzymes ranged from a 13-84% increase over Cel7A alone. The most effective combinations included the addition of both XynA and Axe1, which synergistically enhance xylan conversions resulting in additional synergistic improvements in glucan conversion. Additionally, we note a direct relationship between enzymatic xylan removal in the presence of XynA and the enhancement of cellulose hydrolysis by Cel7A.

Selig, M. J.; Knoshaug E. P.; Adney, W. S.; Himmel, M. E.; Decker, S. R.

2007-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

JGI - Why Sequence Alvinella pompejana?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Pompeii Worm? Pompeii Worm? Alvinella pompeiiana One of the most thermophilic eukaryotes, Alvinella pompejana, the Pompeii worm, is a resident of the Pacific deep-sea hydrothermal vent area. These worms reside on black smoker chimneys 2500 meters under the ocean surface where they experience (1) the highest temperatures and temperature gradients known for any eukaryote (20-80°C), (2) a toxic soup of heavy metals, and (3) very low pH. Thus their environment is perhaps the most extreme known for any eukaryote. This project unites cDNA sequencing, which is crucial for genetic and protein analysis, with macromolecular structure determination by x-ray crystallography, solution small-angle x-ray scattering, and electron microscopy. Proteins from thermophilic sources, currently limited to unicellular bacteria and archaea, have proven to be

422

Biochemistry and physiology of anaerobic bacteria  

SciTech Connect (OSTI)

We welcome you to The Power of Anaerobes. This conference serves two purposes. One is to celebrate the life of Harry D. Peck, Jr.,who was born May 18, 1927 and would have celebrated his 73rd birthday at this conference. He died November 20, 1998. The second is to gather investigators to exchange views within the realm of anaerobic microbiology, an area in which tremendous progress has been seen during recent years. It is sufficient to mention discoveries of a new form of life (the archaea), hyper or extreme thermophiles, thermophilic alkaliphiles and anaerobic fungi. With these discoveries has come a new realization about physiological and metabolic properties of microorganisms, and this in turn has demonstrated their importance for the development, maintenance and sustenance of life on Earth.

NONE

2000-05-18T23:59:59.000Z

423

JGI - Why Sequence Terephthalate-Degrading Microbial Community?  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

a Terephthalate-Degrading Microbial Community? a Terephthalate-Degrading Microbial Community? Every year, enormous amounts of high-strength terephthalate-containing wastewater are produced as a byproduct of the plastics industry. The wastewater is currently treated by using anaerobic biological treatment processes that involve mesophilic (moderate-temperature-loving) microbes growing at 35-37°C. Recently, a thermophilic (heating-loving) terephthalate-degrading community growing at around 55°C has been developed in a lab-scale bioreactor as a more efficient alternative to the mesophilic process. Metagenomic characterization of the thermophilic bioreactor community, followed by perturbation studies, may lead to an optimized process, which will have significant impact on the plastic production industry in terms of wastewater treatment costs and operational

424

Impacts of Shallow Geothermal Energy Production on Redox Processes and Microbial Communities  

Science Journals Connector (OSTI)

Impacts of Shallow Geothermal Energy Production on Redox Processes and Microbial Communities ... Thermophilic sulfate reduction, however, had a higher activation energy (100–160 kJ mol–1) than mesophilic sulfate reduction (30–60 kJ mol–1), which might be due to a trade-off between enzyme stability and activity with thermostable enzymes being less efficient catalysts that require higher activation energies. ...

Matthijs Bonte; Wilfred F. M. Röling; Egija Zaura; Paul W. J. J. van der Wielen; Pieter J. Stuyfzand; Boris M. van Breukelen

2013-11-22T23:59:59.000Z

425

PETROLEUM BIOREFINING FOR POLLUTION PREVENTION  

SciTech Connect (OSTI)

The objective of this project was to isolate and characterize thermophilic bacterial cultures that can be used for the selective removal of nitrogen, sulfur, and/or metals in the biorefining of petroleum. The project was completed on schedule and no major difficulties were encountered. Significant progress was made on multiple topics relevant to the development of a petroleum biorefining process capable of operating at thermophilic temperatures. New cultures capable of selectively cleaving C-N or C-S bonds in molecules relevant to petroleum were obtained, and the genes encoding the enzymes for these unique biochemical reactions were cloned and sequenced. Genetic tools were developed that enable the use of Thermus thermophilus as a host to express any gene of interest, and information was obtained regarding the optimum conditions for the growth of T. thermophilus. The development of a practical biorefining process still requires further research and the future research needs identified in this project include the development of new enzymes and pathways for the selective cleavage of C-N or C-S bonds that have higher specific activities, increased substrate range, and are capable of functioning at thermophilic temperatures. Additionally, there is a need for process engineering research to determine the maximum yield of biomass and cloned gene products that can be obtained in fed-batch cultures using T. thermophilus, and to determine the best configuration for a process employing biocatalysts to treat petroleum.

John J. Kilbane II

2002-03-01T23:59:59.000Z

426

Thermostabilization of desulfurization enzymes from Rhodococcos sp. IGTS8. Final technical report  

SciTech Connect (OSTI)

The objective of this project was to develop thermophilic cultures capable of expressing the desulfurization (dsz) operon of Rhodococcus sp. IGTS8. The approaches taken in this project included the development of plasmid and integrative expression vectors that function well in Thermus thermophilus, the cloning of Rhodococcus dsz genes in Thermus expression vectors, and the isolation of bacterial cultures that express the dsz operon at thermophilic temperatures. This project has resulted in the development of plasmid and integrative expression vectors for use in T. thermophilus. The dsz genes have been expressed at moderately thermophilic temperatures (52 C) in Mycobacterium phlei and at temperatures as high as 72 C in T. thermophilus. The tools and methods developed in this project will be generally useful for the expression of heterologous genes in Thermus. Key developments in the project have been the isolation of a Mycobacterium phlei culture capable of expressing the desulfurization operon at 52 C, development of plasmid and integrative expression vectors for Thermus thermophilus, and the development of a host-vector system based on the malate dehydrogenase gene that allows plasmids to be stably maintained in T. thermophilus and provides a convenient reporter gene for the accurate quantification of gene expression. Publications have been prepared regarding each of these topics; these preprints are included.

John J. Kilbane II

2000-12-15T23:59:59.000Z

427

Protection Motivation Theory and Consumer Willingness-to-Pay, in the Case of Post-Harvest Processed Gulf Oysters  

E-Print Network [OSTI]

.vulnificus refers to a halophilic bacterium naturally occurring in brackish coastal waters, which concentrates in filter-feeding oysters. Proposed FDA legislation requiring processing of all raw Gulf oysters sold during warmer summer months threatens the Gulf...

Blunt, Emily Ann

2012-10-19T23:59:59.000Z

428

Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants  

Science Journals Connector (OSTI)

...III secretion systems. To facilitate future communication, I suggest renaming the...systems will be identified in the near future. The resulting increase in sequence information...bacterium facilitated an evolutionary quantum leap (E. A. Groisman) in bacterial...

Christoph J. Hueck

1998-06-01T23:59:59.000Z

429

Toxicity testing of oil-contaminated drilling cuttings  

Science Journals Connector (OSTI)

The luminescent bacterium Photobacterium phosphoreum...has been used to examine samples of oily drilling cuttings from the sea bottom in the vicinity of a North Sea oil production platform. Because the presence o...

B. Neustadt; I. L. Marr; H. W. Zwanziger

430

Production of Clostridium difficile toxin in a medium totally free of both animal and dairy proteins or digests  

E-Print Network [OSTI]

In the hope of developing a vaccine against Clostridium difficile based on its toxin(s), we have developed a fermentation medium for the bacterium that results in the formation of Toxin A and contains no meat or dairy ...

Demain, Arnold L.

431

Structural characterization of a novel autonomous cohesin from Ruminococcus flavefaciens  

Science Journals Connector (OSTI)

The crystal structure of a standalone cohesin protein from the rumen bacterium R. flavefaciens has been determined at 2.44 ? resolution, revealing an elaborate set of secondary-structural elements.

Voronov-Goldman, M.

2014-03-25T23:59:59.000Z

432

Xanthomonas oryzae pathovars: model pathogens of a model crop.  

E-Print Network [OSTI]

the plant pathogen Xanthomonas campestris pv. vesicatoria.of the plant pathogenic bacterium Xanthomonas campestris pv.pv. oryzae in artificially inoculated and naturally infected rice seeds and plants

Niño-Liu, David O; Ronald, Pamela C; Bogdanove, Adam J

2006-01-01T23:59:59.000Z

433

Detection of streptomycin resistance in Erwinia amylovora strains isolated from apple orchards in Chihuahua, Mexico  

Science Journals Connector (OSTI)

Fire blight, one of the most severe diseases of apple and pear, is caused by the bacterium Erwinia amylovora. One control method is the use of antibiotics like streptomycin; however, streptomycin is the only anti...

Adrian Ponce de León Door; Alejandro Romo Chacón…

2013-10-01T23:59:59.000Z

434

Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function  

Science Journals Connector (OSTI)

...interaction with the exposed surface polysaccharides...CsxA from the cell surface of the bacterium...Chi-CBM35 to the surface of...polygalacturonase treatment reflecting the exposure of xylan epitopes by pectic...

Cedric Montanier; Alicia Lammerts van Bueren; Claire Dumon; James E. Flint; Marcia A. Correia; Jose A. Prates; Susan J. Firbank; Richard J. Lewis; Gilles G. Grondin; Mariana G. Ghinet; Tracey M. Gloster; Cecile Herve; J. Paul Knox; Brian G. Talbot; Johan P. Turkenburg; Janne Kerovuo; Ryszard Brzezinski; Carlos M. G. A. Fontes; Gideon J. Davies; Alisdair B. Boraston; Harry J. Gilbert

2009-01-01T23:59:59.000Z

435

Draft genome sequence of strain HIMB100, a cultured representative of the SAR116 clade of marine Alphaproteobacteria  

E-Print Network [OSTI]

Strain HIMB100 is a planktonic marine bacterium in the class Alphaproteobacteria. This strain is of interest because it is one of the first known isolates from a globally ubiquitous clade of marine bacteria known as SAR116 ...

Grote, Jana

2011-01-01T23:59:59.000Z

436

Postexponential Regulation of sin Operon Expression in Bacillus subtilis  

Science Journals Connector (OSTI)

...of sin Operon Expression in Bacillus subtilis Sasha H. Shafikhani 1 Ines...required during the early stages of Bacillus subtilis sporulation is regulated by...as the common soil bacterium Bacillus subtilis frequently exist in slow- or...

Sasha H. Shafikhani; Ines Mandic-Mulec; Mark A. Strauch; Issar Smith; Terrance Leighton

2002-01-01T23:59:59.000Z

437

Sensing Applications of Fluctuations and Noise  

E-Print Network [OSTI]

Noise and time-dependent fluctuations are usually undesirable signals. However, they have many applications. This dissertation deals with two kinds of sensing applications of fluctuation and noise: soil bulk density assessment and bacterium sensing...

Chang, Hung-Chih

2011-02-22T23:59:59.000Z

438

SREL Reprint #3027  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

sp. nov., an acid-tolerant spore-forming anaerobic bacterium from contructed wetland sediment Yong-Jin Lee,1,3 Christopher S. Romanek,2,3 and Juergen Wiegel1 1Department of...

439

Inflammasome activation causes dual recruitment of NLRC4 and NLRP3 to the same macromolecular complex  

E-Print Network [OSTI]

assembly independently of one another; however, here we show that the important food poisoning bacterium Salmonella enterica serovar Typhimurium (S. Typhimuri- um) simultaneously activates at least two NLRs whilst only a single inflammasome complex...

Ming Man, Si; Hopkins, Lee J; Nugent, Eileen; Cox, Susan; Glück, Ivo M; Tourlomousis, Panagiotis; Wright, John A; Cicuta, Pietro; Monie, Tom P.; Bryant, Clare E.

2014-05-06T23:59:59.000Z

440

Helicobacter pylori moves through mucus by reducing mucin viscoelasticity  

E-Print Network [OSTI]

The ulcer-causing gastric pathogen Helicobacter pylori is the only bacterium known to colonize the harsh acidic environment of the human stomach. H. pylori survives in acidic conditions by producing urease, which catalyzes ...

So, Peter T. C.

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol  

Science Journals Connector (OSTI)

A goal for the food industry has always been to improve strains of Lactococcus lactis...and stabilize beneficial traits. Genetic engineering is used extensively for manipulating this lactic acid bacterium, while ...

Maria Papagianni; Nicholaos Avramidis; George Filioussis

2007-03-01T23:59:59.000Z

442

Discovery of Ethanol-Responsive Small RNAs in Zymomonas mobilis  

Science Journals Connector (OSTI)

...Biotechnology Discovery of Ethanol-Responsive Small RNAs in...bacterium that can produce ethanol by fermentation. Due to its unique metabolism and efficient ethanol production, Z. mobilis has...special interest for biofuel energy applications; an important...

Seung Hee Cho; Roy Lei; Trey D. Henninger; Lydia M. Contreras

2014-05-02T23:59:59.000Z

443

ROLE OF AN ABC TRANSPORTER COMPLEX IN VIOLOGEN TOLERANCE IN STREPTOCOCCUS MUTANS  

E-Print Network [OSTI]

The gram-positive bacterium Streptococcus mutans is the primary causative agent in the formation of dental caries in humans. To persist in the oral cavity, S. mutans must be able to tolerate rapid and substantial environmental ...

Biswas, Saswati

2011-05-31T23:59:59.000Z

444

TRENDS in Genetics Vol.18 No.12 December 2002 http://tig.trends.com 0168-9525/02/$ see front matter 2002 Elsevier Science Ltd. All rights reserved. PII: S0168-9525(02)02793-2  

E-Print Network [OSTI]

repeats and conversion events are the main mechanism of divergence between closely related primate genomes on the biochemical pathways by which the bacterium `reduces' and precipitates chromium, uranium and other toxic

Utrecht, Universiteit

445

WE START COMPANIES THAT CHANGE THE WORLD.  

E-Print Network [OSTI]

throughout North Carolina and across the nation. Agile Sciences has developed nontoxic biofilm dispersants with Bartonella, the bacterium that causes cat scratch disease. The NMR LipoProfile test uses nuclear magnetic

446

Wind speed and wind-associated leaf injury affect severity of citrus canker on Swingle citrumelo  

Science Journals Connector (OSTI)

Citrus canker (caused by the bacterial pathogen Xanthomonas citri subsp. citri, Xcc...) can cause severe damage to citrus. It is endemic in Florida, and occurs in other citrus growing regions. The bacterium is di...

C. H. Bock; James H. Graham; Tim R. Gottwald…

2010-09-01T23:59:59.000Z

447

T cell clones and their products: Experimental clues for the immunoprophylaxis and immunotherapy of intracellular bacterial infections?  

Science Journals Connector (OSTI)

In this paper experimental models utilizing murine T cell clones specific for the intracellular bacteriumListeria monocytogenes are described and some matters of possible relevance to the development of novel imm...

Priv.-Doz. Dr. S. H. E. Kaufmann

1985-01-01T23:59:59.000Z

448

T cell clones and their products: Experimental clues for the immunoprophylaxis and immunotherapy of intracellular bacterial infections?  

Science Journals Connector (OSTI)

In this paper experimental models utilizing murine T cell clones specific for the intracellular bacteriumListeria monocytogenes are described and some matters of possible relevance to the development of novel imm...

Priv.-Doz. Dr. S. H. E. Kaufmann

1984-01-01T23:59:59.000Z

449

Discovery of Ethanol-Responsive Small RNAs in Zymomonas mobilis  

Science Journals Connector (OSTI)

...research-article Biotechnology Discovery of Ethanol-Responsive Small RNAs in Zymomonas...mobilis is a bacterium that can produce ethanol by fermentation. Due to its unique metabolism and efficient ethanol production, Z. mobilis has attracted...

Seung Hee Cho; Roy Lei; Trey D. Henninger; Lydia M. Contreras

2014-05-02T23:59:59.000Z

450

A World of Research REPORT ON RESEARCH 2007  

E-Print Network [OSTI]

developed on campus is also expanding. 2007 saw the spin off of a new Amherst-based company called SunEthanol. Formed around research on a novel bacterium that efficiently converts plant matter to ethanol

Nagurney, Anna

451

Complete Genome Sequences of Bacillus subtilis subsp. subtilis Laboratory Strains JH642 (AG174) and AG1839  

E-Print Network [OSTI]

The Gram-positive bacterium Bacillus subtilis is widely used for studies of cellular and molecular processes. We announce the complete genomic sequences of strain AG174, our stock of the commonly used strain JH642, and ...

Smith, Janet L.

452

Structural Basis for Activation of Cholera Toxin  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2005 00:00 Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio...

453

Comparative Analysis of Microbial Community Composition Throughout Three Perennially Ice-Covered Lake Systems in the McMurdo Dry Valleys, Antarctica and its Relationship With Lake Geochemistry  

E-Print Network [OSTI]

Delta Proteobacteria F12, F18 Uncultured eubacterium AB16 (Delta Proteobacteria F12, F18 Rhodoferax antarcticus strainDelta Proteobacteria F12, F18 Uncultured bacterium clone

Foo, Wilson

2009-01-01T23:59:59.000Z

454

Antimicrobial product and process  

DOE Patents [OSTI]

A composition for controlling a plant disease caused by a plant pathogenic bacterium is disclosed. The composition comprises an activity for inhibiting the growth of the plant pathogenic bacterium and is extracted in an aqueous solvent from particles of malted cereal grain. The composition is used either in dry or wet form by application to plant parts, such as potato seed pieces, that are to be protected from the pathogenic bacteria. 6 figs.

Barrett, K.B.

1997-12-16T23:59:59.000Z

455

Antimicrobial product and process  

DOE Patents [OSTI]

A composition for controlling a plant disease caused by a plant pathogenic bacterium is disclosed. The composition comprises an activity for inhibiting the growth of the plant pathogenic bacterium and is extracted in an aqueous solvent from particles of malted cereal grain. The composition is used either in dry or wet form by application to plant parts, such as potato seed pieces, that are to be protected from the pathogenic bacteria.

Barrett, Karen B. (Idaho Falls, ID)

1997-01-01T23:59:59.000Z

456

A minimal set of bacterial cellulases for consolidated bioprocessing of lignocellulose  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

11 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 11 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 1 Biotechnol. J. 2011, 6 DOI 10.1002/biot.201100157 www.biotechnology-journal.com 1 Introduction Cellulosic material is the most abundant renew- able bioresource. Cellulose biodegradation medi- ated by cellulases and/or cellulolytic microorgan- isms represents one of the largest flows in the global carbon cycle [1]. The utilization of a small fraction of cellulosic materials (e.g., 5-10%) for the production of biofuels and value-added chemicals would greatly decrease reliance on crude oil, pro- mote rural economy, decrease net greenhouse emissions, and increase national energy security [1-3]. Cost-effective release of fermentable sugars from non-food biomass through biomass pretreat- ment/enzymatic hydrolysis is still the largest ob-

457

Microbial diversity of cellulose hydrolysis  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

871; 871; NO. OF PAGES 5 Please cite this article in press as: Wilson DB. Microbial diversity of cellulose hydrolysis, Curr Opin Microbiol (2011), doi:10.1016/j.mib.2011.04.004 Available online at www.sciencedirect.com Microbial diversity of cellulose hydrolysis David B Wilson Enzymatic hydrolysis of cellulose by microorganisms is a key step in the global carbon cycle. Despite its abundance only a small percentage of microorganisms can degrade cellulose, probably because it is present in recalcitrant cell walls. There are at least five distinct mechanisms used by different microorganisms to degrade cellulose all of which involve cellulases. Cellulolytic organisms and cellulases are extremely diverse possibly because their natural substrates, plant cell walls, are very diverse. At this time the microbial ecology of cellulose degradation in any environment is still

458

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

1, 1, p. 4042-4054 Vol. 77, No. 12 0099-2240/11/$12.00 doi:10.1128/AEM.02811-10 Copyright © 2011, American Society for Microbiology. All Rights Reserved. Use of Label-Free Quantitative Proteomics To Distinguish the Secreted Cellulolytic Systems of Caldicellulosiruptor bescii and Caldicellulosiruptor obsidiansis ᰔ † Adriane Lochner, 1,3,4 Richard J. Giannone, 2,3 Miguel Rodriguez, Jr., 1,3 Manesh B. Shah, 2 Jonathan R. Mielenz, 1,3 Martin Keller, 1,3 Garabed Antranikian, 4 David E. Graham, 1,5 * and Robert L. Hettich 2,3 * Biosciences Division, 1 Chemical Sciences Division, 2 and BioEnergy Science Center, 3 Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831; Technical Microbiology, Hamburg University of Technology, Kasernenstrasse 12, D-21073 Hamburg, Germany 4 ; and Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996 5 Received

459

BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS The  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The The noncellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity Xiao-Zhou Zhang & Zuoming Zhang & Zhiguang Zhu & Noppadon Sathitsuksanoh & Yunfeng Yang & Y.-H. Percival Zhang Received: 21 July 2009 / Revised: 29 August 2009 / Accepted: 31 August 2009 / Published online: 15 October 2009 # Springer-Verlag 2009 Abstract Family 48 glycoside hydrolases (cellobiohydro- lases) are among the most important cellulase components for crystalline cellulose hydrolysis mediated by cellulolytic bacteria. Open reading frame (Cphy_3368) of Clostridium phytofermentans ISDg encodes a putative family 48 glycoside hydrolase (CpCel48) with a family 3 cellulose- binding module. CpCel48 was successfully expressed as two soluble intracellular forms with or without a C-terminal

460

AEM.01219-10v1.pdf  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

KHUPRSKLOLF $QDHURELF %DFWHULD IURP %LRFRPSRVW $EOH WR 'HJUDGH KHUPRSKLOLF $QDHURELF %DFWHULD IURP %LRFRPSRVW $EOH WR 'HJUDGH 1 &HOOXORVH DQG ;\ODQ 2 Cellulolytic and Xylanolytic Bacteria 3 0 9 6L]RYD   -$ ,]TXLHUGR   16 3DQLNRY  DQG / 5 /\QG̅  4 1 Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755 5 2 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 6 3 Department of Biology, Northeastern University, Boston, Massachusetts 02115 7 ̅Corresponding author: Lee R. Lynd 8 E-mail: Lee.R.Lynd@Dartmouth.EDU 9 Phone: 603-646-2231 10 Fax: 603-646-2277 11 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Development of a commercial enzymes system for lignocellulosic biomass saccharification  

SciTech Connect (OSTI)

DSM Innovation Inc., in its four year effort was able to evaluate and develop its in-house DSM fungal cellulolytic enzymes system to reach enzyme efficiency mandates set by DoE Biomass program MYPP goals. DSM enzyme cocktail is uniquely active at high temperature and acidic pH, offering many benefits and product differentiation in 2G bioethanol production. Under this project, strain and process development, ratio optimization of enzymes, protein and genetic engineering has led to multitudes of improvement in productivity and efficiency making development of a commercial enzyme system for lignocellulosic biomass saccharification viable. DSM is continuing further improvement by additional biodiversity screening, protein engineering and overexpression of enzymes to continue to further lower the cost of enzymes for saccharification of biomass.

Manoj Kumar

2012-12-20T23:59:59.000Z

462

doi:10.1016/j.copbio.2008.04.008  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

A A biophysical perspective on the cellulosome: new opportunities for biomass conversion Shi-You Ding 1 , Qi Xu 1 , Michael Crowley 1 , Yining Zeng 1 , Mark Nimlos 2 , Raphael Lamed 3 , Edward A Bayer 4 and Michael E Himmel 1 The cellulosome is a multiprotein complex, produced primarily by anaerobic microorganisms, which functions to degrade lignocellulosic materials. An important topic of current debate is whether cellulosomal systems display greater ability to deconstruct complex biomass materials (e.g. plant cell walls) than nonaggregated enzymes, and in so doing would be appropriate for improved, commercial bioconversion processes. To sufficiently understand the complex macromolecular processes between plant cell wall polymers, cellulolytic microbes, and their secreted enzymes, a highly concerted research approach is required. Adaptation of existing biophysical techniques

463

Comparison of different liquid anaerobic digestion effluents as inocula and nitrogen sources for solid-state batch anaerobic digestion of corn stover  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer Compared methane production of solid AD inoculated with different effluents. Black-Right-Pointing-Pointer Food waste effluent (FWE) had the largest population of acetoclastic methanogens. Black-Right-Pointing-Pointer Solid AD inoculated with FWE produced the highest methane yield at F/E ratio of 4. Black-Right-Pointing-Pointer Dairy waste effluent (DWE) was rich of cellulolytic and xylanolytic bacteria. Black-Right-Pointing-Pointer Solid AD inoculated with DWE produced the highest methane yield at F/E ratio of 2. - Abstract: Effluents from three liquid anaerobic digesters, fed with municipal sewage sludge, food waste, or dairy waste, were evaluated as inocula and nitrogen sources for solid-state batch anaerobic digestion of corn stover in mesophilic reactors. Three feedstock-to-effluent (F/E) ratios (i.e., 2, 4, and 6) were tested for each effluent. At an F/E ratio of 2, the reactor inoculated by dairy waste effluent achieved the highest methane yield of 238.5 L/kgVS{sub feed}, while at an F/E ratio of 4, the reactor inoculated by food waste effluent achieved the highest methane yield of 199.6 L/kgVS{sub feed}. The microbial population and chemical composition of the three effluents were substantially different. Food waste effluent had the largest population of acetoclastic methanogens, while dairy waste effluent had the largest populations of cellulolytic and xylanolytic bacteria. Dairy waste also had the highest C/N ratio of 8.5 and the highest alkalinity of 19.3 g CaCO{sub 3}/kg. The performance of solid-state batch anaerobic digestion reactors was closely related to the microbial status in the liquid anaerobic digestion effluents.

Xu Fuqing; Shi Jian [Department of Food, Agricultural and Biological Engineering, Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691 (United States); Lv Wen; Yu Zhongtang [Department of Animal Sciences, Ohio State University, Columbus, OH 43210 (United States); Li Yebo, E-mail: li.851@osu.edu [Department of Food, Agricultural and Biological Engineering, Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691 (United States)

2013-01-15T23:59:59.000Z

464

Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis  

SciTech Connect (OSTI)

Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred to as white rot fungi. Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. To investigate the basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora and P. chrysosporium. Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium, respectively. In addition, the C. subvermispora genome contains at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains none. We also observed expansion of the number of C. subvermispora desaturase-encoding genes putatively involved in lipid metabolism. Microarray-based transcriptome analysis showed substantial up-regulation of several desaturase and MnP genes in wood-containing medium. MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. These results support the importance of MnP and a lignin degradation mechanism whereby cleavage of the dominant nonphenolic structures is mediated by lipid peroxidation products. Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and, following heterologous expression in Escherichia coli, the enzymes were shown to oxidize high redox potential substrates, but not Mn2. Apart from oxidative lignin degradation, we also examined cellulolytic and hemicellulolytic systems in both fungi. In summary, the C. subvermispora genetic inventory and expression patterns exhibit increased oxidoreductase potential and diminished cellulolytic capability relative to P. chrysosporium.

Fernandez-Fueyo, Elena; Ruiz-Duenas, Francisco J.; Ferreira, Patrica; Floudas, Dimitrios; HIbbett, David S.; Canessa, Paulo; Larrondo, Luis F.; James, Tim Y.; Seelenfreund, Daniela; Lobos, Sergio; Polanco, Ruben; Tello, Mario; Honda, Yoichi; Watanabe, Takahito; Watanabe, Takashi; Ryu, Jae San; Kubicek, Christian P.; Schmoll, Monika; Gaskell, Jill; Hammel, Kenneth E.; St. John, Franz J.; Vanden Wymelenberg, Amber; Sabat, Grzegorz; Splinter BonDurant, Sandra; Syed, Khajamohiddin; Yadav, Jagjit S.; Doddapaneni, Harshavardhan; Subramanian, Venkataramanan; Lavin, Jose L.; Oguiza, Jose A.; Perez, Gumer; Pisabarro, Antonio G.; Ramirez, Lucia; Santoyo, Francisco; Master, Emma; Coutinho, Pedro M.; Henrissat, Bernard; Lombard, Vincent; Magnuson, Jon Karl; Kues, Ursula; Hori, Chiaki; Igarashi, Kiyohiko; Samejima, Masahiro; Held, Benjamin W.; Barry, Kerrie W.; LaButti, Kurt M.; Lapidus, Alla; Lindquist, Erika A.; Lucas, Susan M.; Riley, Robert; Salamov, Asaf A.; Hoffmeister, Dirk; Schwenk, Daniel; Hadar, Yitzhak; Yarden, Oded; de Vries, Ronald P.; Wiebenga, Ad; Stenlid, Jan; Eastwood, Daniel; Grigoriev, Igor V.; Berka, Randy M.; Blanchette, Robert A.; Kersten, Phil; Martinez, Angel T.; Vicuna, Rafael; Cullen, Dan

2011-12-06T23:59:59.000Z

465

High ethanol producing derivatives of Thermoanaerobacter ethanolicus  

DOE Patents [OSTI]

Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).

Ljungdahl, Lars G. (Athens, GA); Carriera, Laura H. (Athens, GA)

1983-01-01T23:59:59.000Z

466

High ethanol producing derivatives of Thermoanaerobacter ethanolicus  

DOE Patents [OSTI]

Derivatives of the newly discovered microorganism Thermoanaerobacter ethanolicus which under anaerobic and thermophilic conditions continuously ferment substrates such as starch, cellobiose, glucose, xylose and other sugars to produce recoverable amounts of ethanol solving the problem of fermentations yielding low concentrations of ethanol using the parent strain of the microorganism Thermoanaerobacter ethanolicus are disclosed. These new derivatives are ethanol tolerant up to 10% (v/v) ethanol during fermentation. The process includes the use of an aqueous fermentation medium, containing the substrate at a substrate concentration greater than 1% (w/v).

Ljungdahl, L.G.; Carriera, L.H.

1983-05-24T23:59:59.000Z

467

Complete genome sequence of Thermosediminibacter oceani type strain (JW/IW-1228PT)  

SciTech Connect (OSTI)

Thermosediminibacter oceani (Lee et al. 2006) is the type species of the genus Thermosediminibacter in the family Thermoanaerobacteraceae. The anaerobic, barophilic, chemoorganotrophic thermophile is characterized by straight to curved Gram-negative rods. The strain described in this study has been isolated from a core sample of deep sea sediments of the Peruvian high productivity upwelling system. This is the first completed genome sequence of a member of the genus Thermosediminibacter and the seventh genome sequence in the family Thermoanaerobacteraceae. The 2,280,035 bp long genome with its 2,285 protein-coding and 63 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Yasawong, Montri [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Munk, Christine [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Bruce, David [U.S. Department of Energy, Joint Genome Institute; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Liolios, Konstantinos [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Chang, Yun-Juan [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Sikorski, Johannes [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

2010-01-01T23:59:59.000Z

468

Cellulase producing microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1997-01-01T23:59:59.000Z

469

Cellulase producing microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1997-12-30T23:59:59.000Z

470

Method of producing a cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulose-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1998-05-26T23:59:59.000Z

471

Cellulase-containing cell-free fermentate produced from microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1997-12-16T23:59:59.000Z

472

Detergent composition comprising a cellulase containing cell-free fermentate produced from microorganism ATCC 55702 or mutant thereof  

DOE Patents [OSTI]

Bacteria which produce large amounts of a cellulase-containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualities for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1998-07-14T23:59:59.000Z

473

Temporal variation in methanogen communities of four different full-scale anaerobic digesters treating food waste-recycling wastewater  

Science Journals Connector (OSTI)

Abstract Methanogen communities were investigated using 454 pyrosequencing in four different full-scale anaerobic digesters treating food waste-recycling wastewater. Seasonal samples were collected for 2 years, and 24 samples were available for microbial analysis from a plug flow thermophilic (PT) digester, a continuously-stirred tank thermophilic (CT) digester, an upflow anerobic sludge blanket mesophilic (UM) digester, and a continuously-stirred tank mesophilic (CM) digester. Methanoculleus, Methanobacterium, Methanothermobacter, and Methanosaeta were revealed to be key methanogens in full-scale anaerobic digestion process treating food waste-recycling wastewater. In the PT digester, Methanoculleus was dominant (96.8%). In the CT digester, Methanoculleus was dominant (95.4%) during the first year of operation, but the dominant genus was shifted to Methanothermobacter (98.5%) due to pH increase. In the UM digester, Methanosaeta was dominant (87.2%). In the CM digester, Methanoculleus was constantly dominant (74.8%) except during CM5 when Methanosaeta was dominant (62.6%) due to the low residual acetate concentration (0.1 g/L).

Joonyeob Lee; Byungchul Hwang; Taewoan Koo; Seung Gu Shin; Woong Kim; Seokhwan Hwang

2014-01-01T23:59:59.000Z

474

The crystal structure of the conserved \\{GTPase\\} of SRP54 from the archaeon Acidianus ambivalens and its comparison with related structures suggests a model for the SRP–SRP receptor complex  

Science Journals Connector (OSTI)

Background: Protein targeting to the endoplasmic reticulum in eukaryotes and to the cell membrane in prokaryotes is mediated by the signal recognition particle (SRP) and its receptor (SR). Both contain conserved \\{GTPase\\} domains in the signal-peptide-binding proteins (SRP54 and Ffh) and the SR proteins (SR? and FtsY). These \\{GTPases\\} are involved in the regulation of protein targeting. Most studies so far have focussed on the SRP machinery of mammals and bacteria, leaving the SRP system of archaea less well understood. Results: We report the crystal structure of the conserved \\{GTPase\\} (NG-Ffh) from the thermophilic archaeon Acidianus ambivalens at 2.0 Å resolution and of the Thr112?Ala mutant, which is inactive in GTP hydrolysis. This is the first structure of an SRP component from an archaeon and allows for a detailed comparison with related structures from Escherichia coli and thermophilic bacteria. In particular, differences in the conserved consensus regions for nucleotide binding and the subdomain interfaces are observed, which provide information about the regulation of the GTPase. These interactions allow us to propose a common signalling mechanism for the SRP–SR system. Conclusions: The overall structure of SRP-GTPases is well conserved between bacteria and archaea, which indicates strong similarities in the regulation of the SRP-targeting pathway. Surprisingly, structure comparisons identified a homodimeric ATP-binding protein as the closest relative. A heterodimer model for the SRP–SR interaction is presented.

Guillermo Montoya; Kai te Kaat; Ralph Moll; Günter Schäfer; Irmgard Sinning

2000-01-01T23:59:59.000Z

475

Probing the mechanism of rubredoxin thermal unfolding in the absence of salt bridges by temperature jump experiments  

SciTech Connect (OSTI)

Rubredoxins are the simplest type of iron-sulphur proteins and in recent years they have been used as model systems in protein folding and stability studies, especially the proteins from thermophilic sources. Here, we report our studies on the rubredoxin from the hyperthermophile Methanococcus jannaschii (T {sub opt} = 85 deg C), which was investigated in respect to its thermal unfolding kinetics by temperature jump experiments. Different spectroscopic probes were used to monitor distinct structural protein features during the thermal transition: the integrity of the iron-sulphur centre was monitored by visible absorption spectroscopy, whereas tertiary structure was followed by intrinsic tryptophan fluorescence and exposure of protein hydrophobic patches was sensed by 1-anilinonaphthalene-8-sulphonate fluorescence. The studies were performed at acidic pH conditions in which any stabilising contributions from salt bridges are annulled due to protonation of protein side chain groups. In these conditions, M. jannaschii rubredoxin assumes a native-like, albeit more flexible and open conformation, as indicated by a red shift in the tryptophan emission maximum and 1-anilinonaphthalene-8-sulphonate binding. Temperature jumps were monitored by the three distinct techniques and showed that the protein undergoes thermal denaturation via a simple two step mechanism, as loss of tertiary structure, hydrophobic collapse, and disintegration of the iron-sulphur centre are concomitant processes. The proposed mechanism is framed with the multiphasic one proposed for Pyrococcus furiosus rubredoxin, showing that a common thermal unfolding mechanism is not observed between these two closely related thermophilic rubredoxins.

Henriques, Barbara J. [Instituto Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras (Portugal); Saraiva, Ligia M. [Instituto Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras (Portugal); Gomes, Claudio M. [Instituto Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras (Portugal)]. E-mail: gomes@itqb.unl.pt

2005-08-05T23:59:59.000Z

476

Marine Fisheries Review Vol. 40, No, 10  

E-Print Network [OSTI]

., Seattle, WA 98105. Publication of material from sources outside the Service is not an en- dorsement of the Lobster, Homarl/S americanus James E. S,ewart 5 A New Bacterium (Presumptive Vibrio Species) Causing: An Abstract Louis LeibovilZ 9 Vibriosis in Maine and ~ew Hampshire Salmonids Evelyn S. Sawyer 10 Anaerobic

477

Heavy Metal Tolerance in Stenotrophomonas maltophilia Delphine Pages1,2,3  

E-Print Network [OSTI]

Heavy Metal Tolerance in Stenotrophomonas maltophilia Delphine Pages1,2,3 , Jerome Rose4 , Sandrine, this bacterium tolerates high levels (0.1 to 50 mM) of various toxic metals, such as Cd, Pb, Co, Zn, Hg, Ag mechanisms to overcome metal toxicity, reduction of oxyanions to non-toxic elemental ions and detoxification

Paris-Sud XI, Université de

478

Groceries trip triclosan switch  

Science Journals Connector (OSTI)

... Triclosan interferes with an enzyme crucial to the growth of bacteria. But it also trips ... host of unwanted chemicals. "It's a bacterium's vomit response," explains Gilbert. Triclosan opponents such as Levy are unconvinced. "I don't see a health relevance to ...

Tom Clarke

2001-05-25T23:59:59.000Z

479

Dissipative shocks behind bacteria gliding  

Science Journals Connector (OSTI)

...In (3.22), W 0 is the power supply at the bacterium pore...W s is the concentrated power supply involved in the dissipative...internal shock at the filaments foot, given by (2.7), W g is the power expended by the gravitational...

2014-01-01T23:59:59.000Z

480

Campylobacter jejuni Motility Is Required for Infection of the Flagellotropic Bacteriophage F341  

Science Journals Connector (OSTI)

...infection is observed as intracellular green fluorescence. (C) C. jejuni NCTC11168...min post-infection with phage F341. Green fluorescence covers the bacterium, indicating...YP Li, DD Bang and H Ingmer. 2009. Energy taxis drives Campylobacter jejuni toward...

Signe Berg Baldvinsson; Martine C. Holst Sørensen; Christina S. Vegge; Martha R. J. Clokie; Lone Brøndsted

2014-09-26T23:59:59.000Z

Note: This page contains sample records for the topic "thermophilic cellulolytic bacterium" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Effective viscosity of bacterial suspensions: a three-dimensional PDE model with stochastic torque.  

SciTech Connect (OSTI)

We present a PDE model for dilute suspensions of swimming bacteria in a three-dimensional Stokesian fluid. This model is used to calculate the statistically-stationary bulk deviatoric stress and effective viscosity of the suspension from the microscopic details of the interaction of an elongated body with the background flow. A bacterium is modeled as an impenetrable prolate spheroid with self-propulsion provided by a point force, which appears in the model as an inhomogeneous delta function in the PDE. The bacterium is also subject to a stochastic torque in order to model tumbling (random reorientation). Due to a bacterium's asymmetric shape, interactions with prescribed generic planar background flows, such as a pure straining or planar shear flow, cause the bacterium to preferentially align in certain directions. Due to the stochastic torque, the steady-state distribution of orientations is unique for a given background flow. Under this distribution of orientations, self-propulsion produces a reduction in the effective viscosity. For sufficiently weak background flows, the effect of self-propulsion on the effective viscosity dominates all other contributions, leading to an effective viscosity of the suspension that is lower than the viscosity of the ambient fluid. This is in qualitative agreement with recent experiments on suspensions of Bacillus subtilis.

Haines, B. M.; Aranson, I. S.; Berlyand, L.; Karpeev, D. A. (Mathematics and Computer Science); ( MSD); (Penn State Univ.)

2012-01-01T23:59:59.000Z

482

EHS 7-7 August 2007 DEPARTMENT OF ENVIRONMENTAL HEALTH & SAFETY  

E-Print Network [OSTI]

EHS 7-7 August 2007 DEPARTMENT OF ENVIRONMENTAL HEALTH & SAFETY Biological Safety Office 1200 subjects? Yes No Recombinant DNA molecules host cells Check one: Plant* Animal* Bacterium Fungus Animal or animal pathogens. Vector to be used: Describe the inserted recombinant DNA materials: (if applicable

Sura, Philip

483

Methods for targetted mutagenesis in gram-positive bacteria  

SciTech Connect (OSTI)

The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

Yang, Yunfeng

2014-05-27T23:59:59.000Z

484

2,4-Dichlorophenol Degradation Using Streptomyces viridosporus T7A Lignin Peroxidase  

E-Print Network [OSTI]

2,4-Dichlorophenol Degradation Using Streptomyces viridosporus T7A Lignin Peroxidase Dennis C. Yee, California 92697-2575 The Streptomyces viridosporus T7A bacterium produces the extracellular lignin from the ping- pong bireactant system that is typically used for horseradish peroxidase and lignin

Wood, Thomas K.

485

Genome Sequence of Mercury-Methylating and Pleomorphic Desulfovibrio africanus strain Walvis Bay  

SciTech Connect (OSTI)

Desulfovibrio africanus strain Walvis Bay is an anaerobic sulfate-reducing bacterium capable of producing methylmercury (MeHg), a potent human neurotoxin. The mechanism of methylation by this and other organisms is unknown. We present the 4.2-Mb genome sequence to provide further insight into microbial mercury methylation and sulfate-reducing bacteria.

Brown, Steven D [ORNL; Wall, Judy D. [University of Missouri; Kucken, A M [University of Missouri, Columbia; Gilmour, C C [Smithsonian Environmental Research Center, Edgewater, MD; Podar, Mircea [ORNL; Brandt, Craig C [ORNL; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Detter, J C [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Han, James [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Len [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Pitluck, Samual [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [ORNL; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Palumbo, Anthony Vito [ORNL; Elias, Dwayne A [ORNL

2011-01-01T23:59:59.000Z

486

Complete Genome Sequence of the Haloalkaliphilic, Hydrogen Producing Halanaerobium hydrogenoformans  

SciTech Connect (OSTI)

Halanaerobium hydrogenoformans is an alkaliphilic bacterium capable of biohydrogen production at pH 11 and 7% (w/v) salt. We present the 2.6 Mb genome sequence to provide insights into its physiology and potential for bioenergy applications.

Brown, Steven D [ORNL; Begemann, Matthew B [University of Wisconsin, Madison; Mormile, Dr. Melanie R. [Missouri University of Science and Technology; Wall, Judy D. [University of Missouri; Han, Cliff [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Samual [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Elias, Dwayne A [ORNL

2011-01-01T23:59:59.000Z

487

Genome Sequence of Streptomyces viridosporus Strain T7A ATCC 39115, a Lignin-Degrading Actinomycete  

SciTech Connect (OSTI)

We announce the availability of the genome sequence of Streptomyces viridosporus strain T7A ATCC 39115, a plant biomass- degrading actinomycete. This bacterium is of special interest because of its capacity to degrade lignin, an underutilized compo- nent of plants in the context of bioenergy. It has a full complement of genes for plant biomass catabolism.

Davis, Jennifer R. [Brown University; Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Teshima, Hazuki [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Huntemann, Marcel [U.S. Department of Energy, Joint Genome Institute; Wei, Chia-Lin [Los Alamos National Laboratory (LANL); Han, James [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Szeto, Ernest [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Pagani, Ioanna [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Peters, Lin [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Land, Miriam L [ORNL; Sello, Jason K. [Brown University

2013-01-01T23:59:59.000Z

488

The Complete Genome Sequence and Updated Annotation of Desulfovibrio alaskensis G20  

SciTech Connect (OSTI)

Desulfovibrio alaskensis G20 (formerly desulfuricans G20) is a Gram-negative mesophilic sulfate-reducing bacterium (SRB), known to corrode ferrous metals and to reduce toxic radionuclides and metals such as uranium and chromium to sparingly soluble and less toxic forms. We present the 3.7 Mb genome sequence to provide insights into its physiology.

Hauser, Loren John [ORNL; Wall, Judy D. [University of Missouri; Brown, Steven D [ORNL; Land, Miriam L [ORNL; Bruce, David [U.S. Department of Energy, Joint Genome Institute; Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Frank, Larimer [Oak Ridge National Laboratory (ORNL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Palumbo, Anthony Vito [ORNL; Pitluck, Samual [U.S. Department of Energy, Joint Genome Institute; Keller, Kimberly L [University of Missouri, Columbia; Rapp-Giles, Barbara J [University of Missouri, Columbia; Price, Morgan N. [Lawrence Berkeley National Laboratory (LBNL); Lin, Monica [University of California, Berkeley; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Copeland, A [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute

2011-01-01T23:59:59.000Z

489

Ion selectivity of the Vibrio alginolyticus flagellar motor.  

Science Journals Connector (OSTI)

...Vibrio alginolyticus flagellar motor. J Z Liu M Dapice S Khan Department...ion transfers limit unloaded motor speed in this bacterium and...to flagellar rotation. The motor is composed of several independent...obtained after the profile of the electric field has been crossed by the...

J Z Liu; M Dapice; S Khan

1990-09-01T23:59:59.000Z

490

Spatially-Correlated Mass Spectrometric Analysis of Microbe-Mineral Interactions  

SciTech Connect (OSTI)

A new methodology for examining the interactions of microbes with heterogeneous minerals is presented. Imaging laser-desorption Fourier transform mass spectrometry was used to examine the colonization patterns of Burkholderia vietnamiensis (Burkholderia cepacia) G4 on a heterogeneous basalt sample. Depth-profile imaging found that the bacterium preferentially colonized the plagioclase mineral phases within the basalt.

Jill R. Scott; Beizhan Yan; Daphne L. Stoner

2006-11-01T23:59:59.000Z

491

Role of iron, capsule, and toxins in the pathogenicity of Vibrio vulnificus biotype 2 for mice.  

Science Journals Connector (OSTI)

...pathogen (4). Biotype 1 is a marine bacterium, not virulent for...and other lactose-fermenting marine vibrios in coastal waters of...lactose-fermenting vibrios in the marine environment. Appl. Environ...Oliver, J. D., J. E. Wear, M. B. Thomas, M. Warner...

C Amaro; E G Biosca; B Fouz; A E Toranzo; E Garay

1994-02-01T23:59:59.000Z

492

Adherence of Pseudomonas aeruginosa to hydrophilic contact lenses and other substrata.  

Science Journals Connector (OSTI)

...associated with contact lens wear. Am. J. Ophthalmol...primary film-forming marine bacterium. Dev. Ind...associated with contact lens wear. Arch. Ophthalmol...events in the sorption of marine bacteria to surfaces...ulcers and contact lens wear. J. Clin. Microbiol...

M J Miller; D G Ahearn

1987-08-01T23:59:59.000Z

493

Hydrogen, Fuel Cells, and Infrastructure Technologies FY 2002 Progress Report Section II. Hydrogen Production and Delivery  

E-Print Network [OSTI]

.A Biological Processes II.A.1 Biological Water Gas Shift Development Gary R. Vanzin, Sharon Smolinski, Karen the overall water-gas shift (WGS) pathway by serving as an inducer, a substrate, and an inhibitor. · Elucidate the specific CO shift kinetics of the photosynthetic bacterium Rubrivivax (Rx.) gelatinosus CBS. Approach

494

Identification of Polyphosphate-Accumulating Organisms and Design of 16S rRNA-Directed Probes for Their Detection and Quantitation  

Science Journals Connector (OSTI)

...The performance of reactor GRC fluctuated over...Microbiological analyses. (i) Microscopy of...Enrichment, phylogenetic analysis and detection of a bacterium...Kerdachi K. J. Healey The reliability of cold perchloric acid...Salkinoja-Salonen Comparative analysis of biological phosphate...

Gregory R. Crocetti; Philip Hugenholtz; Philip L. Bond; Andrew Schuler; Jürg Keller; David Jenkins; Linda L. Blackall

2000-03-01T23:59:59.000Z

495

Biodegradation of Aromatic Compounds byEscherichia coli  

Science Journals Connector (OSTI)

...depletion of the cellular pool of this amino acid...The tnaAB genes make up an operon that is...in the catabolic pool of this enteric bacterium...processes in two-liquid water-solvent systems...contamination in water and soil samples...appropriate approach to reduce the transfer of recombinant...

Eduardo D??az; Abel Ferrández; Mar??a A. Prieto; José L. Garc??a

2001-12-01T23:59:59.000Z

496

Appears in the Proceedings of the 1991 DARPA Workshop on CaseBased Reasoning, MorganKaufmann. Finding Genes by CaseBased Reasoning  

E-Print Network [OSTI]

Department of Genetics that is sequencing the DNA of the bacterium E. coli. 1. Introduction Given a new Sciences Department University of Wisconsin Madison, WI 53706 USA shavlik@cs.wisc.edu Abstract Effectively error rates. This system is being used in conjunction with a Human Genome project in the Wisconsin

Liblit, Ben

497

Laser spotlight reveals machine 'climbing' DNA New imaging technology has revealed how the molecular machines that remodel genetic  

E-Print Network [OSTI]

, MukBEF (which is made from several different protein molecules), inside the bacterium E.coli. DavidBEF, effectively creating a single dye tag for each component of these machines. Up until now conventional at the very tiny length scale for understanding the causes of many diseases in humans, and how to devise new

Leake, Mark C.

498

Appears in the Proceedings of the 1991 DARPA Workshop on Case-Based Reasoning, Morgan-Kaufmann. Finding Genes by Case-Based Reasoning  

E-Print Network [OSTI]

Department of Genetics that is sequencing the DNA of the bacterium E. coli. 1. Introduction Given a new Sciences Department University of Wisconsin Madison, WI 53706 USA shavlik@cs.wisc.edu Abstract Effectively error rates. This system is being used in conjunction with a Human Genome project in the Wisconsin

Shavlik, Jude W.

499

Evolution in materio: Looking Beyond the Silicon Box Julian F. Miller Keith Downing  

E-Print Network [OSTI]

Escherichia coli (E. coli). This is a single-celled bacterium. It doesn't have a nucleus (prokaryotic not normally associated with electronic functions. Electronic components have been prefected by human designers. Artificial evolution may be much more effective when the configurable medium has a rich and complicated

Fernandez, Thomas

500

Received 21 Feb 2014 | Accepted 27 Mar 2014 | Published 29 Apr 2014 Mutation rate plasticity in rifampicin resistance  

E-Print Network [OSTI]

physiological responses, adaptive or otherwise, affecting mutation rate. Here, using E. coli, we identify both between3,4 and locally within5,6 genotypes. In particular systems, such as the model bacterium E. coli, there is abundant variation in mutation rates among natural isolates7. For a single genotype

Channon, Alastair