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Sample records for telephone cellular fax

  1. Cellular telephone-based radiation detection instrument

    DOE Patents [OSTI]

    Craig, William W.; Labov, Simon E.

    2011-06-14

    A network of radiation detection instruments, each having a small solid state radiation sensor module integrated into a cellular phone for providing radiation detection data and analysis directly to a user. The sensor module includes a solid-state crystal bonded to an ASIC readout providing a low cost, low power, light weight compact instrument to detect and measure radiation energies in the local ambient radiation field. In particular, the photon energy, time of event, and location of the detection instrument at the time of detection is recorded for real time transmission to a central data collection/analysis system. The collected data from the entire network of radiation detection instruments are combined by intelligent correlation/analysis algorithms which map the background radiation and detect, identify and track radiation anomalies in the region.

  2. Cellular telephone-based wide-area radiation detection network

    DOE Patents [OSTI]

    Craig, William W.; Labov, Simon E.

    2009-06-09

    A network of radiation detection instruments, each having a small solid state radiation sensor module integrated into a cellular phone for providing radiation detection data and analysis directly to a user. The sensor module includes a solid-state crystal bonded to an ASIC readout providing a low cost, low power, light weight compact instrument to detect and measure radiation energies in the local ambient radiation field. In particular, the photon energy, time of event, and location of the detection instrument at the time of detection is recorded for real time transmission to a central data collection/analysis system. The collected data from the entire network of radiation detection instruments are combined by intelligent correlation/analysis algorithms which map the background radiation and detect, identify and track radiation anomalies in the region.

  3. FAX

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FATIGUE DESIGN CURVES FOR 6061-T6 ALUMINUM* G . T . Yahr Engineering Technology Division Oak Ridge National Laboratory Oak Ridge, Tennessee 37831-8051 ABSTRACT A request has been made to the ASME Boiler and Pressure Vessel Committee that 6061-T6 aluminum be approved for use in the construction of Class 1 welded nuclear vessels so it can be used for the pressure vessel of the Advanced Neutron Source research reactor. Fatigue design curves with and without mean stress effects have been proposed. A

  4. First Name: Last Name: Title: Telephone: Fax: Email: First Name...

    U.S. Energy Information Administration (EIA) Indexed Site

    NOTICE: This report is mandatory under the Federal Energy Administration Act of 1974 (Public Law 93-275). Failure to comply may result in criminal fines, civil penalties and other ...

  5. Cellular telephone-based radiation sensor and wide-area detection network

    DOE Patents [OSTI]

    Craig, William W.; Labov, Simon E.

    2006-12-12

    A network of radiation detection instruments, each having a small solid state radiation sensor module integrated into a cellular phone for providing radiation detection data and analysis directly to a user. The sensor module includes a solid-state crystal bonded to an ASIC readout providing a low cost, low power, light weight compact instrument to detect and measure radiation energies in the local ambient radiation field. In particular, the photon energy, time of event, and location of the detection instrument at the time of detection is recorded for real time transmission to a central data collection/analysis system. The collected data from the entire network of radiation detection instruments are combined by intelligent correlation/analysis algorithms which map the background radiation and detect, identify and track radiation anomalies in the region.

  6. CARLSBAD ENVIRONMENTAL MONITORING & RESEARCH CENTER NEW MEXICO STATE UNIVERSITY TELEPHONE (575) 887-2759

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ENVIRONMENTAL MONITORING & RESEARCH CENTER NEW MEXICO STATE UNIVERSITY TELEPHONE (575) 887-2759 1400 UNIVERSITY DRIVE, CARLSBAD, NEW MEXICO 88220 FAX NUMBER (575) 887-3051 An Update on CEMRC radiological results from air and surface water sampling activities following the February 14 th , 2014 radiation detection event The Carlsbad Environmental Monitoring and Research Center (CEMRC), an entity of New Mexico State University, continues to conduct radiological separation and analyses on a

  7. Telephone Service | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Telephone Service Forward your telephone calls to an alternate number and access your voice mail from off-site. Host a Conference Call All employees can use BlueJeans to host conference calls. You can also request a conference call setup by submitting an online request to the phone operators at least 1 hour before the conference start time. Requests are processed and confirmations are sent during normal business hours. You will also need to supply an Argonne cost code. Submit a Teleconference

  8. Alaska Power Telephone Company | Open Energy Information

    Open Energy Info (EERE)

    search Name: Alaska Power Telephone Company Address: 193 Otto Street PO Box 3222 Place: Port Townsend Zip: 98368 Region: United States Sector: Marine and Hydrokinetic Phone Number:...

  9. DOE - Office of Legacy Management -- Bell Telephone Laboratories...

    Office of Legacy Management (LM)

    Bell Telephone Laboratories - Murray Hill - NJ 0-04 FUSRAP Considered Sites Site: BELL TELEPHONE LABORATORIES - MURRAY HILL (NJ.0-04 ) Eliminated from consideration under FUSRAP...

  10. The Telephone: An Invention with Many Fathers

    ScienceCinema (OSTI)

    Brenni, Paolo [CNR-FST-IMSS, Florence, Italy

    2010-01-08

    The names of A.G. Bell, A. Meucci, P.Reis, E. Gray, just to mention the most important ones, are all connected with the invention of the telephone. Today, the Italian inventor A. Meucci is recognized as being the first to propose a working prototype of the electric telephone. However, for a series of reasons his strenuous efforts were not rewarded. I will not repeat here the endless and complex disputes about the ?real father? of the telephone. From an historical point of view it is more interesting to understand why so many individuals from different backgrounds conceived of a similar apparatus and why most of these devices were simply forgotten or just remained laboratory curiosities. The case of the development of the telephone is an emblematic and useful example for better understanding the intricate factors which are involved in the birth of an invention and reasons for its success and failure.

  11. SBOT OKLAHOMA SOUTHWESTERN POWER ADMIN POC Gary Bridges Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    OKLAHOMA SOUTHWESTERN POWER ADMIN POC Gary Bridges Telephone (918) 595-6671 Email gary.bridges@swpa...

  12. Full page fax print

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Other major miscellaneous inputs Internal to the Tank Farm ... Salt crystallized from high-heat waste and low-heat waste is ... The rotary kiln primary combustion chamber will be used ...

  13. Full page fax print

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5 (U) April 26, 1995 Westinghouse Savannah River Company Savannah River S~e Aiken, SC 29808 HLW-OVP-95-0031 @ Westinghouse Savannah River Company. April 27, 1995 A. B. Scoa, Jr. Va Preoident and General...,. Hgh Level Was .. ManagamenI DiviIion P. O. Box 61' Allen, SC 29602 HLW-OVP-95-0031 Keywords: Retention: High Level W88te SyslemPIan Production Plan 25 years, offer to NARA at end of retention. Disposal Auth: DOEF 1324.5 95-0002 Track .: 7605 File Record: 773-52A, Records Mr. Steven D.

  14. Fax Cover Sheet

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Council) Noah Horowitz (Natural Resources Defense Council) (by phone) Jennifer Thorne Amann (American Council for an Energy-Efficient Economy) Neal Goldberg (National Cable ...

  15. Full page fax print

    National Nuclear Security Administration (NNSA)

  16. Full page fax print

    National Nuclear Security Administration (NNSA)

  17. Full page fax print

    Office of Legacy Management (LM)

  18. Full page fax print

    Office of Scientific and Technical Information (OSTI)

    )lIJfjlHfllmj)mmllillruJt ((I(m(ti.JmlmmmlllWI ll .. . I*mw lffi )( HI I) SOVELEV RECELEV 40 40 Immlll1 illllmJil11)Immm lfm lli m l.lmillm(mj...

  19. UTILITIES COLORADO WESTERN POWER ADMIN POC Cheryl Drake Telephone

    Energy Savers [EERE]

    UTILITIES COLORADO WESTERN POWER ADMIN POC Cheryl Drake Telephone (720) 962-7154 Email drake@wapa.gov Electric Bulk Power Transmission and Control 221121 Electric Power Distribution 221122 GEORGIA SOUTHEASTERN POWER ADMIN POC Ann Craft Telephone (706) 213-3823 Email annc@sepa.doe.gov Electric Bulk Power Transmission and Control 221121 Electric Power Distribution 221122 OKLAHOMA SOUTHWESTERN POWER ADMIN POC Gary Bridges Telephone (918) 595-6671 Email gary.bridges@swpa.gov Electric Bulk Power

  20. EDUCATION CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone

    Energy Savers [EERE]

    EDUCATION CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone (510) 486-4506 Email dtchen@lbl.gov Computer Training 611420 Professional and Management Development Training 611430 LAWRENCE LIVERMORE LAB POC Jill Swanson Telephone (925) 423-4535 Email swanson6@llnl.gov Computer Training 611420 Professional and Management Development Training 611430 COLORADO GOLDEN FIELD OFFICE POC Karen Downs Telephone (720) 356-1269 Email karen.downs@go.doe.gov Computer Training 611420 Professional and

  1. CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... All Other Professional, Scientific, and Technical Services 541990 NEVADA NEVADA SITE OFFICE POC Anita Ross Telephone (702) 295-5690 Email rossal@nv.doe.gov Other Accounting ...

  2. FORESTRY COLORADO WESTERN POWER ADMIN POC Cheryl Drake Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FORESTRY COLORADO WESTERN POWER ADMIN POC Cheryl Drake Telephone (720) 962-7154 Email drake@wapa.gov Timber tract operations 113110 Cutting and transporting timber 113310 GEORGIA ...

  3. SBOT TEXAS PANTEX PLANT POC Brad Beck Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    PANTEX PLANT POC Brad Beck Telephone (806) 477-6192 Email bbrack@pantex.com ADMINISTATIVE WASTE REMEDIATION Facilities Support Services 561210 Executive Search Services 561312 ...

  4. SBOT SOUTH CAROLINA SAVANNAH RIVER LAB POC Sharon Campbell Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SOUTH CAROLINA SAVANNAH RIVER LAB POC Sharon Campbell Telephone (800) 888-7986 Email sharon-pmmd.campbell@srs.gov EDUCATION Professional and Management Development Training 611430 ...

  5. Systems configured to distribute a telephone call, communication systems, communication methods and methods of routing a telephone call to a service representative

    DOE Patents [OSTI]

    Harris, Scott H.; Johnson, Joel A.; Neiswanger, Jeffery R.; Twitchell, Kevin E.

    2004-03-09

    The present invention includes systems configured to distribute a telephone call, communication systems, communication methods and methods of routing a telephone call to a customer service representative. In one embodiment of the invention, a system configured to distribute a telephone call within a network includes a distributor adapted to connect with a telephone system, the distributor being configured to connect a telephone call using the telephone system and output the telephone call and associated data of the telephone call; and a plurality of customer service representative terminals connected with the distributor and a selected customer service representative terminal being configured to receive the telephone call and the associated data, the distributor and the selected customer service representative terminal being configured to synchronize, application of the telephone call and associated data from the distributor to the selected customer service representative terminal.

  6. COLORADO GOLDEN FIELD OFFICE POC Karen Downs Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Long-Distance 484230 WESTERN POWER ADMIN POC Cheryl Drake Telephone (720) 962-7154 Email drake@wapa.gov General Freight Trucking, Local 484110 General Freight Trucking, Long-Di...

  7. Stratified random sampling plan for an irrigation customer telephone survey

    SciTech Connect (OSTI)

    Johnston, J.W.; Davis, L.J.

    1986-05-01

    This report describes the procedures used to design and select a sample for a telephone survey of individuals who use electricity in irrigating agricultural cropland in the Pacific Northwest. The survey is intended to gather information on the irrigated agricultural sector that will be useful for conservation assessment, load forecasting, rate design, and other regional power planning activities.

  8. CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone

    Energy Savers [EERE]

    PROFESSIONAL / SCIENTIFIC / TECHNICAL CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone (510) 486-4506 Email dtchen@lbl.gov Engineering Services 541330 Drafting Services 541340 Geophysical Surveying and Mapping Services 541360 Testing Laboratories 541380 Custom Computer Programming Services 541511 Computer Systems Design Services 541512 Other Computer Related Services 541519 Administrative Management and General Management Consulting Services 541611 Other Scientific and Technical

  9. Consensual Listening-in to or Recording Telephone/Radio Conversations (restricted)

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1992-11-12

    This Order specifies the Department of Energy (DOE) policy regarding the consensual listening-in to or recording of conversations on radio and telephone systems. Canceled by DOE N 251.107.

  10. > FAQs for Survey Form EIA-888

    Gasoline and Diesel Fuel Update (EIA)

    Fax - fax price(s) to a secure, toll-free fax number, 1-877-359-6637 Telephone ... of the Federal Energy Administration Act of 1974 (FEAA) (Public Law 93-275), as amended. ...

  11. FAQs for Survey Form EIA-878

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Fax - fax prices to a secure, toll-free fax number, 1-877-359-6637 Telephone ... of the Federal Energy Administration Act of 1974 (FEAA) (Public Law 93-275), as amended. ...

  12. Eva Stringer | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Administrative Secretary Eva Stringer Telephone 630.252.6889 Fax 630.252.4646 E-mail stringer

  13. Nozomi Shirato | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Assistant Physicst Nozomi Shirato Telephone 630.252.3605 Fax 630.252.5739 E-mail nshirato

  14. Kathy Vanoskey | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kathy Vanoskey Administrative Secretary Telephone 630.252.4106 Fax 630. 252.6866 E-mail kvanoskey

  15. Supratik Guha | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Supratik Guha NST Division Director Telephone 630.252.7740 Fax 630.252.6866 E-mail sguha

  16. Rachel Mirelez | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Administrative Secretary Rachel Mirelez Telephone 630.252.4651 Fax 630.252.6866 E-mail rmirelez

  17. Naryanan (Bobby) Kasthuri | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Neuroscience Researcher Naryanan (Bobby) Kasthuri Telephone 630.252.4966 Fax 630.252.5739 E-mail bobbykasthuri...

  18. Telephone Flat Geothermal Development Project Environmental Impact Statement Environmental Impact Report. Final: Comments and Responses to Comments

    SciTech Connect (OSTI)

    1999-02-01

    This document is the Comments and Responses to Comments volume of the Final Environmental Impact Statement and Environmental Impact Report prepared for the proposed Telephone Flat Geothermal Development Project (Final EIS/EIR). This volume of the Final EIS/EIR provides copies of the written comments received on the Draft EIS/EIR and the leady agency responses to those comments in conformance with the requirements of the National Environmental Policy Act (NEPA) and the California Environmental Quality Act (CEQA).

  19. Mechanisms of cellular transformation by carcinogenic agents

    SciTech Connect (OSTI)

    Grunberger, D.; Goff, S.P.

    1987-01-01

    This book contains 14 chapters. Some of the chapter titles are: DNA Modification by Chemical Carcinogens; Role of DNA Lesions and Repair in the Transformation of Human Cells; The Induction and Regulation of Radiogenic Transformation In Vitro: Cellular and Molecular Mechanisms; Cellular Transformation by Adenoviruses; and The fos Gene.

  20. River Corridor Closure Contract Section J, Attachment...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    use (i.e., use outside the employees regular schedule) of government property (such as computers, telephones, copiers, fax machines or other office equipment) or commercially...

  1. Brandon Fisher | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Engineering Specialist Senior Brandon Fisher News Atomically Thin Metallic Boron Telephone 630.252.5324 Fax 630.252.4646 E-mail bfisher@anl.gov

  2. External Technical Review Report

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Telephone, internet and Fax access. Siteproject clearance ... List of initial information needed TITLE:

  3. Jonathan Logan | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdoctoral Appointee (Supervisor, Ian McNulty) Jonathan Logan Telephone 630.252.4874 Fax 630.252.5739 E-mail jmlogan

  4. Nancy Dietz-Rago | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Materials Scientist Nancy Dietz-Rago Telephone 630.252.9798 Fax 630.252.5739 E-mail dietz@anl.gov

  5. Tal Heilpern | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdoctoral Appointee (Supervisor, Stephen Gray) Tal Heilpern Telephone 630.252.5884 Fax 630.252.4646 E-mail theilpern

  6. Letter Report Final SA _01-17-01_.PDF

    Energy Savers [EERE]

    01 Constitution Avenue, NW, HA274, Washington, DC 20418 Telephone (202) 334 3376 Fax (202) 334 3370 Board on Infrastructure and the Constructed Environment January 17, 2001...

  7. Laurie Eichberger | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Executive Secretary Laurie Eichberger Telephone 630.252.7570 Fax 630.252.6866 E-mail eichberger@anl.gov

  8. Dean Carbaugh | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Senior Technician Dean Carbaugh Telephone 630.252.4833 Fax 630.252.5739 E-mail dcarbaugh@anl.gov

  9. Deborah O'Rourke | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Deborah O'Rourke NST Assistant Division Director Telephone 630.252.3810 Fax 630.252.6866 E-mail dorourke

  10. EEOICPA_Handbook_DRAFT_2_1_2013

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Telephone: (904) 357-4705 Fax: (904) 357-4704 Toll Free: (877) 336-4272 Regional Director: Maggie Fernandez District Director: Jim Bibeault Assistant District Director: David ...

  11. Joonseok Lee | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fellow (Supervisor, Elena Roshkova) News Piezoelectrically enhanced ferroelectric polymers via nanoscale mechanical annealing Telephone 630.252.7317 Fax 630.252.4646 E-mail...

  12. Alper Kinaci | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Postdoctoral Appointee (Supervisor, Maria Chan) Alper Kinaci Telephone 630.252.5378 Fax 630.252.4646 E-mail akinaci

  13. Property:Incentive/ContFax | Open Energy Information

    Open Energy Info (EERE)

    Regulations: No.27 - Control of Nitrogen Oxide Emissions (Rhode Island) + 401-222-2017 + Air Quality (Nova Scotia, Canada) + (902) 424-0503 + Air Quality Approvals and Permits...

  14. BPA-2014-00385-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    BOX 398 * HEPPNER, OREGON 97836-0398 Telephone (541) 676-9146 * Fax (541) 676-5159 Condon Telephone (541) 384-2023 jerryh@coIumbabasin.cc tommyw@columbiabasin.cc...

  15. BPA-2014-00386-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    WAY * P.O. BOX 398 * HEPPNER, OREGON Telephone (541) 676-9146 * Fax (541) 676-5159 Condon Telephone (541) 384-2023 97836-0398 jerryh@cokimbiabasin.cc tommyw@colmbiabasin.cc...

  16. BPA-2014-00383-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    BOX 398 * HEPPNERI OREGON 97836-0398 Telephone (541) 676.9146 * Fax (541) 676-5159 Condon Telephone (541) 384-2023 jerryh@columbiabasin.cc tommyw@columbiabasin.cc...

  17. BPA-2014-00384-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    WAY * P.O. BOX 398 * HEPPNER, OREGON Telephone (541) 676-9146 * Fax (541) 676-5159 Condon Telephone (541) 384-2023 97836-0398 jerryh@columbiabasin.cc tommyw@colurnbiabasin.cc...

  18. BPA-2014-00387-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    BOX 398 * HEPPNER, OREGON 97836-0398 Telephone (541) 676-9146 * Fax (541) 676-5159 Condon Telephone (541) 384-2023 jerryh@columbiabasin.cc tommyw@coumbabasin.cc...

  19. BPA-2014-00382-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    BOX 398 * HEPPNER, OREGON 97836-0398 Telephone (541) 676-9146 * Fax (541) 676-5159 Condon Telephone (541) 384-2023 jeh@coumbabasin,cc tommyw@columbiabasin.cc...

  20. Fax and Evaluation Letter: Fax transmits the following letter: Post-Cleanup Evaluation for the Southeast Drainage, February 12, 1999.

    Office of Legacy Management (LM)

  1. On the reversibility of transitions between closed and open cellular...

    Office of Scientific and Technical Information (OSTI)

    between closed and open cellular convection Citation Details In-Document Search Title: On the reversibility of transitions between closed and open cellular convection The ...

  2. Toxicology and cellular effect of manufactured nanomaterials

    DOE Patents [OSTI]

    Chen, Fanqing

    2014-07-22

    The increasing use of nanotechnology in consumer products and medical applications underlies the importance of understanding its potential toxic effects to people and the environment. Herein are described methods and assays to predict and evaluate the cellular effects of nanomaterial exposure. Exposing cells to nanomaterials at cytotoxic doses induces cell cycle arrest and increases apoptosis/necrosis, activates genes involved in cellular transport, metabolism, cell cycle regulation, and stress response. Certain nanomaterials induce genes indicative of a strong immune and inflammatory response within skin fibroblasts. Furthermore, the described multiwall carbon nanoonions (MWCNOs) can be used as a therapeutic in the treatment of cancer due to its cytotoxicity.

  3. Algorithmic crystal chemistry: A cellular automata approach

    SciTech Connect (OSTI)

    Krivovichev, S. V.

    2012-01-15

    Atomic-molecular mechanisms of crystal growth can be modeled based on crystallochemical information using cellular automata (a particular case of finite deterministic automata). In particular, the formation of heteropolyhedral layered complexes in uranyl selenates can be modeled applying a one-dimensional three-colored cellular automaton. The use of the theory of calculations (in particular, the theory of automata) in crystallography allows one to interpret crystal growth as a computational process (the realization of an algorithm or program with a finite number of steps).

  4. Microsoft Word - frm-1901rev1.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Form FRM-1901 ASBESTIFORM SHIPMENT NOTIFICATION Fax to (702) 295-1153 02/14/08 Rev. 01 Page 1 of 1 The generator signed below shall submit this notification to NNSA/NSO at least seven days in advance of arrival of shipment of Asbestiform low-level waste to the Area 5 Radioactive Waste Management Site (RWMS). Generator: Generator Address: Generator Telephone Number: Fax Number: Transporter Name: Transporter Address: Transporter Telephone Number: Fax Number: Waste Stream Identification Number:

  5. Cellular responses to environmental DNA damage

    SciTech Connect (OSTI)

    Not Available

    1994-08-01

    This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

  6. Cellular membrane trafficking of mesoporous silica nanoparticles

    SciTech Connect (OSTI)

    Fang, I-Ju

    2012-06-21

    This dissertation mainly focuses on the investigation of the cellular membrane trafficking of mesoporous silica nanoparticles. We are interested in the study of endocytosis and exocytosis behaviors of mesoporous silica nanoparticles with desired surface functionality. The relationship between mesoporous silica nanoparticles and membrane trafficking of cells, either cancerous cells or normal cells was examined. Since mesoporous silica nanoparticles were applied in many drug delivery cases, the endocytotic efficiency of mesoporous silica nanoparticles needs to be investigated in more details in order to design the cellular drug delivery system in the controlled way. It is well known that cells can engulf some molecules outside of the cells through a receptor-ligand associated endocytosis. We are interested to determine if those biomolecules binding to cell surface receptors can be utilized on mesoporous silica nanoparticle materials to improve the uptake efficiency or govern the mechanism of endocytosis of mesoporous silica nanoparticles. Arginine-glycine-aspartate (RGD) is a small peptide recognized by cell integrin receptors and it was reported that avidin internalization was highly promoted by tumor lectin. Both RGD and avidin were linked to the surface of mesoporous silica nanoparticle materials to investigate the effect of receptor-associated biomolecule on cellular endocytosis efficiency. The effect of ligand types, ligand conformation and ligand density were discussed in Chapter 2 and 3. Furthermore, the exocytosis of mesoporous silica nanoparticles is very attractive for biological applications. The cellular protein sequestration study of mesoporous silica nanoparticles was examined for further information of the intracellular pathway of endocytosed mesoporous silica nanoparticle materials. The surface functionality of mesoporous silica nanoparticle materials demonstrated selectivity among the materials and cancer and normal cell lines. We aimed to determine the specific organelle that mesoporous silica nanoparticles could approach via the identification of harvested proteins from exocytosis process. Based on the study of endo- and exocytosis behavior of mesoporous silica nanoparticle materials, we can design smarter drug delivery vehicles for cancer therapy that can be effectively controlled. The destination, uptake efficiency and the cellular distribution of mesoporous silica nanoparticle materials can be programmable. As a result, release mechanism and release rate of drug delivery systems can be a well-controlled process. The deep investigation of an endo- and exocytosis study of mesoporous silica nanoparticle materials promotes the development of drug delivery applications.

  7. On the reversibility of transitions between closed and open cellular

    Office of Scientific and Technical Information (OSTI)

    convection (Journal Article) | SciTech Connect On the reversibility of transitions between closed and open cellular convection Citation Details In-Document Search Title: On the reversibility of transitions between closed and open cellular convection The two-way transition between closed and open cellular convection is addressed in an idealized cloud-resolving modeling framework. A series of cloud-resolving simulations shows that the transition between closed and open cellular states is

  8. On the reversibility of transitions between closed and open cellular

    Office of Scientific and Technical Information (OSTI)

    convection (Journal Article) | SciTech Connect On the reversibility of transitions between closed and open cellular convection Citation Details In-Document Search Title: On the reversibility of transitions between closed and open cellular convection The two-way transition between closed and open cellular convection is addressed in an idealized cloud resolving modeling framework. A series of cloud resolving simulations shows that the transition between closed and open cellular states is

  9. Evaluation of Cellular Shades in the PNNL Lab Homes

    SciTech Connect (OSTI)

    Petersen, Joseph M.; Sullivan, Greg; Cort, Katherine A.; Merzouk, Massine B.; Weber, Jessica M.

    2015-10-28

    Understand the HVAC energy impact due to scheduled operation of Hunter Douglas cellular shades in the PNNL lab homes.

  10. Cellular membrane collapse by atmospheric-pressure plasma jet

    SciTech Connect (OSTI)

    Kim, Kangil; Sik Yang, Sang E-mail: ssyang@ajou.ac.kr; Jun Ahn, Hak; Lee, Jong-Soo E-mail: ssyang@ajou.ac.kr; Lee, Jae-Hyeok; Kim, Jae-Ho

    2014-01-06

    Cellular membrane dysfunction caused by air plasma in cancer cells has been studied to exploit atmospheric-pressure plasma jets for cancer therapy. Here, we report that plasma jet treatment of cervical cancer HeLa cells increased electrical conductivity across the cellular lipid membrane and caused simultaneous lipid oxidation and cellular membrane collapse. We made this finding by employing a self-manufactured microelectrode chip. Furthermore, increased roughness of the cellular lipid membrane and sequential collapse of the membrane were observed by atomic force microscopy following plasma jet treatment. These results suggest that the cellular membrane catastrophe occurs via coincident altered electrical conductivity, lipid oxidation, and membrane roughening caused by an atmospheric-pressure plasma jet, possibly resulting in cellular vulnerability to reactive species generated from the plasma as well as cytotoxicity to cancer cells.

  11. On the Interaction between Marine Boundary Layer Cellular Cloudiness and

    Office of Scientific and Technical Information (OSTI)

    Surface Heat Fluxes (Journal Article) | SciTech Connect On the Interaction between Marine Boundary Layer Cellular Cloudiness and Surface Heat Fluxes Citation Details In-Document Search Title: On the Interaction between Marine Boundary Layer Cellular Cloudiness and Surface Heat Fluxes The interaction between marine boundary layer cellular cloudiness and surface uxes of sensible and latent heat is investigated. The investigation focuses on the non-precipitating closed-cell state and the

  12. Real-Time Bioluminescent Tracking of Cellular Population Dynamics...

    Office of Scientific and Technical Information (OSTI)

    cellular aliquots followed by extrapolation to the total population size, or through the monitoring of signal intensity from any number of externally stimulated reporter proteins. ...

  13. Evaluation of Cellular Shades in the PNNL Lab Homes (Technical...

    Office of Scientific and Technical Information (OSTI)

    Sponsoring Org: USDOE Country of Publication: United States Language: English Subject: 32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATION Hunter; Douglas; Cellular; Shades; HVAC; ...

  14. A Nanocrystal Sensor for Luminescence Detection of Cellular Forces...

    Office of Scientific and Technical Information (OSTI)

    States Language: English Subject: 77 NANOSCIENCE AND NANOTECHNOLOGY; 47 OTHER INSTRUMENTATION tetrapod stress gauge, luminescent nanocrystals, cellular forces Word Cloud More...

  15. A Nanocrystal Sensor for Luminescence Detection of Cellular Forces

    SciTech Connect (OSTI)

    Choi, Charina; Chou, Jonathan; Lutker, Katie; Werb, Zena; Alivisatos, Paul

    2011-09-29

    Quantum dots have been used as bright fluorescent tags with high photostability to probe numerous biological systems. In this work we present the tetrapod quantum dot as a dynamic, next-generation nanocrystal probe that fluorescently reports cellular forces with spatial and temporal resolution. Its small size and colloidal state suggest that the tetrapod may be further developed as a tool to measure cellular forces in vivo and with macromolecular spatial resolution.

  16. Scientists ratchet up understanding of cellular protein factory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Understanding of cellular protein factory Scientists ratchet up understanding of cellular protein factory The research could aid in development of new antibiotics used to fight multidrug resistant superbugs such as MRSA found in many U.S. hospitals. December 2, 2010 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to

  17. Marcia A. Wood | Argonne National Laboratory

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    Marcia A. Wood Group Leader, Information Solutions and Technology Assurance B.S. Computer Science, University of St. Francis Telephone 630.252.4656 Fax 630.252.6866 E-mail...

  18. Muge Acik | Argonne National Laboratory

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    Muge Acik Argonne Scholar - ANL Named Postdoc (Joseph Katz Fellow); (Supervisor, Seth Darling) News Employee Spotlight: Muge Acik Telephone 630.252.2734 Fax 630.252.4646 E-mail...

  19. Edward Barry | Argonne National Laboratory

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    Edward Barry Argonne Scholar - ANL Named Postdoc (Joseph Katz Fellow); (Supervisor, Seth Darling) Telephone 630.252.6289 Fax 630.252.4646 E-mail edb@anl.gov CV/Resume PDF icon Barry, Edward - Resume.pdf

  20. Staff Listing - Office of Regulation and International Engagement...

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    DC 20585 Director of the Office of Regulation and International Engagement John A. Anderson, Director Room 3E-052 Telephone (202) 586-0521 FAX (202) 586-6050 Division of...

  1. City of Port Townsend Office of City Manager

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    of Port Townsend Office of City Manager 250 Madison Street, 2, Port Townsend, WA 98368 Telephone: (360) 379-5047 Fax: (360) 385-4290 June 27, 2008 Mr. Mark Gendron Vice President...

  2. Quick Facts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Contact Information Administrator: Kenneth E. Legg Headquarters: 1166 Athens Tech Road Elberton, GA 30635-6711 Telephone: 706-213-3800 FAX: 706-213-3884 Marketing Area Georgia, ...

  3. LSU EFRC - Center for Atomic Level Catalyst Design - Contact...

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    Contact Us >> space control Center for Atomic-Level Catalyst Design (CALCD) 110 Chemical ... Telephone: 225.578.1426 Fax: 225.578.1476 E-mail: jjspivey@lsu.edu space control Webmaster ...

  4. --No Title--

    Gasoline and Diesel Fuel Update (EIA)

    (PADD) levels, regions of the country, sub-PADD levels, and the state of California are released by the end of the day through email list, the Web, Fax, and telephone hotline. ...

  5. Aftab Ahmed | Argonne National Laboratory

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    Aftab Ahmed Postdoctoral Appointee (Supervisor, Jeff Guest) Telephone 630.252.2441 Fax 630.252.4646 E-mail aahmed@anl.gov CV/Resume PDF icon Ahmed, Aftab - Bio.pdf

  6. QROU Questions Fax: Fax transmits questions on the Quarry Residuals Operable Unit - Remedial Investigation Report (QROU - RI) for technical meeting set for August 14, 1997.

    Office of Legacy Management (LM)

  7. EE/CA Letter and Fax: Fax transmits a copy of a Weldon Spring Citizens Commission letter regarding the Engineering Evaluation Cost Assessment on the Southeast Drainage.

    Office of Legacy Management (LM)

  8. Real-Time Bioluminescent Tracking of Cellular Population Dynamics

    SciTech Connect (OSTI)

    Close, Dan; Sayler, Gary Steven; Xu, Tingting; Ripp, Steven Anthony

    2014-01-01

    Cellular population dynamics are routinely monitored across many diverse fields for a variety of purposes. In general, these dynamics are assayed either through the direct counting of cellular aliquots followed by extrapolation to the total population size, or through the monitoring of signal intensity from any number of externally stimulated reporter proteins. While both viable methods, here we describe a novel technique that allows for the automated, non-destructive tracking of cellular population dynamics in real-time. This method, which relies on the detection of a continuous bioluminescent signal produced through expression of the bacterial luciferase gene cassette, provides a low cost, low time-intensive means for generating additional data compared to alternative methods.

  9. Oscillatory cellular patterns in three-dimensional directional solidification

    SciTech Connect (OSTI)

    Tourret, D.; Debierre, J. -M.; Song, Y.; Mota, F. L.; Bergeon, N.; Guerin, R.; Trivedi, R.; Billia, B.; Karma, A.

    2015-09-11

    We present a phase-field study of oscillatory breathing modes observed during the solidification of three-dimensional cellular arrays in micro-gravity. Directional solidification experiments conducted onboard the International Space Station have allowed for the first time to observe spatially extended homogeneous arrays of cells and dendrites while minimizing the amount of gravity-induced convection in the liquid. In situ observations of transparent alloys have revealed the existence, over a narrow range of control parameters, of oscillations in cellular arrays with a period ranging from about 25 to 125 minutes. Cellular patterns are spatially disordered, and the oscillations of individual cells are spatiotemporally uncorrelated at long distance. However, in regions displaying short-range spatial ordering, groups of cells can synchronize into oscillatory breathing modes. Quantitative phase-field simulations show that the oscillatory behavior of cells in this regime is linked to a stability limit of the spacing in hexagonal cellular array structures. For relatively high cellular front undercooling (\\ie low growth velocity or high thermal gradient), a gap appears in the otherwise continuous range of stable array spacings. Close to this gap, a sustained oscillatory regime appears with a period that compares quantitatively well with experiment. For control parameters where this gap exist, oscillations typically occur for spacings at the edge of the gap. However, after a change of growth conditions, oscillations can also occur for nearby values of control parameters where this gap just closes and a continuous range of spacings exists. In addition, sustained oscillations at to the opening of this stable gap exhibit a slow periodic modulation of the phase-shift among cells with a slower period of several hours. While long-range coherence of breathing modes can be achieved in simulations for a perfect spatial arrangement of cells as initial condition, global disorder is observed in both three-dimensional experiments and simulations from realistic noisy initial conditions. The, erratic tip splitting events promoted by large amplitude oscillations contribute to maintaining the long-range array disorder, unlike in thin sample experiments where long-range coherence of oscillations is experimentally observable.

  10. Oscillatory cellular patterns in three-dimensional directional solidification

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tourret, D.; Debierre, J. -M.; Song, Y.; Mota, F. L.; Bergeon, N.; Guerin, R.; Trivedi, R.; Billia, B.; Karma, A.

    2015-09-11

    We present a phase-field study of oscillatory breathing modes observed during the solidification of three-dimensional cellular arrays in micro-gravity. Directional solidification experiments conducted onboard the International Space Station have allowed for the first time to observe spatially extended homogeneous arrays of cells and dendrites while minimizing the amount of gravity-induced convection in the liquid. In situ observations of transparent alloys have revealed the existence, over a narrow range of control parameters, of oscillations in cellular arrays with a period ranging from about 25 to 125 minutes. Cellular patterns are spatially disordered, and the oscillations of individual cells are spatiotemporally uncorrelatedmore » at long distance. However, in regions displaying short-range spatial ordering, groups of cells can synchronize into oscillatory breathing modes. Quantitative phase-field simulations show that the oscillatory behavior of cells in this regime is linked to a stability limit of the spacing in hexagonal cellular array structures. For relatively high cellular front undercooling (\\ie low growth velocity or high thermal gradient), a gap appears in the otherwise continuous range of stable array spacings. Close to this gap, a sustained oscillatory regime appears with a period that compares quantitatively well with experiment. For control parameters where this gap exist, oscillations typically occur for spacings at the edge of the gap. However, after a change of growth conditions, oscillations can also occur for nearby values of control parameters where this gap just closes and a continuous range of spacings exists. In addition, sustained oscillations at to the opening of this stable gap exhibit a slow periodic modulation of the phase-shift among cells with a slower period of several hours. While long-range coherence of breathing modes can be achieved in simulations for a perfect spatial arrangement of cells as initial condition, global disorder is observed in both three-dimensional experiments and simulations from realistic noisy initial conditions. The, erratic tip splitting events promoted by large amplitude oscillations contribute to maintaining the long-range array disorder, unlike in thin sample experiments where long-range coherence of oscillations is experimentally observable.« less

  11. This form may be submitted to the EIA by mail, fax, e-mail, or...

    U.S. Energy Information Administration (EIA) Indexed Site

    business on the web.) To use this service, we recommend the use of Microsoft Internet Explorer 5.5 or later or Netscape 4.77 or later. Send your surveys using this secure...

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    U.S. Energy Information Administration (EIA) Indexed Site

    over the web using secure, encrypted processes. (It is the same method that commercial companies communicate with customers when transacting business on the web.) To use this ...

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    over the web using secure, encrypted processes. (It is the same method that commercial companies use to communicate with customers when transacting business on the web.) To use ...

  14. This form may be submitted to the EIA by mail, fax, e-mail, or...

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    month." " since the last report, enter an ""X"" in the block:" " ",,,..."Mo",,,"Yea... "If this is a resubmission, enter an ""X"" in the block:",,,...

  15. This form may be submitted to the EIA by mail, fax, e-mail, or...

    U.S. Energy Information Administration (EIA) Indexed Site

    ,,," Version No: 2014.001" "ANNUAL REPORT OF THE ORIGIN OF NATURAL GAS LIQUIDS PRODUCTION" "FORM EIA-64A" "REPORT YEAR 2014" "This report is...

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    ...www.eia.govsurveyformeia782alist782a.pdf" "Phone No.:",,,..."Ex... you are reporting:" "Type of Report (Check One):" ,,"Original",,,..."Mo",,,"Da...

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    U.S. Energy Information Administration (EIA) Indexed Site

    under the Federal Energy Administration Act of 1974 (Public Law 93-275). Failure to comply may result in criminal fines, civil penalties and other sanctions as provided by law. ...

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    U.S. Energy Information Administration (EIA) Indexed Site

    Failure to comply may result in criminal fines, civil penalties and other sanctions as provided by law. Title 18 USC 1001 makes it a criminal offense for any person knowingly and ...

  19. This form may be submitted to the EIA by mail, fax, e-mail, or...

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    "REPORT YEAR 2011" "This report is mandatory under Public Law 93-275. Failure to comply may result in criminal fines, civil penalties and other sanctions as provided by law. ...

  20. Investigation of Cellular Interactions of Nanoparticles by Helium Ion Microscopy

    SciTech Connect (OSTI)

    Arey, Bruce W.; Shutthanandan, V.; Xie, Yumei; Tolic, Ana; Williams, Nolann G.; Orr, Galya

    2011-06-01

    The helium ion mircroscope (HIM) probes light elements (e.g. C, N, O, P) with high contrast due to the large variation in secondary electron yield, which minimizes the necessity of specimen staining. A defining characteristic of HIM is its remarkable capability to neutralize charge by the implementation of an electron flood gun, which eliminates the need for coating non-conductive specimens for imaging at high resolution. In addition, the small convergence angle in HeIM offers a large depth of field (~5x FE-SEM), enabling tall structures to be viewed in focus within a single image. Taking advantage of these capabilities, we investigate the interactions of engineered nanoparticles (NPs) at the surface of alveolar type II epithelial cells grown at the air-liquid interface (ALI). The increasing use of nanomaterials in a wide range of commercial applications has the potential to increase human exposure to these materials, but the impact of such exposure on human health is still unclear. One of the main routs of exposure is the respiratory tract, where alveolar epithelial cells present a vulnerable target at the interface with ambient air. Since the cellular interactions of NPs govern the cellular response and ultimately determine the impact on human health, our studies will help delineating relationships between particle properties and cellular interactions and response to better evaluate NP toxicity or biocompatibility. The Rutherford backscattered ion (RBI) is a helium ions imaging mode, which backscatters helium ions from every element except hydrogen, with a backscatter yield that depends on the atomic number of the target. Energy-sensitive backscatter analysis is being developed, which when combined with RBI image information, supports elemental identification at helium ion nanometer resolution. This capability will enable distinguishing NPs from cell surface structures with nanometer resolution.

  1. FORMATION BY IRRADIATION OF AN EXPANDED, CELLULAR, POLYMERIC BODY

    DOE Patents [OSTI]

    Charlesby, A.; Ross, M.

    1958-12-01

    The treatment of polymeric esters of methacrylic acid having a softening polnt above 40 icient laborato C to form an expanded cellular mass with a smooth skin is discussed. The disclosed method comprises the steps of subjecting the body at a temperature below the softenpoint to a dose of at least 5 x lO/sup 6/ roentgen of gamma radiation from cobalt-60 source until its average molecular weight is reduced to a value within the range of 3 x lO/sup 5/ to 10/sup 4/, and heating at a temperature within the range of 0 to lO icient laborato C above its softening point to effect expansion.

  2. CANCELLED EMT and back again: does cellular plasticity fuelneoplasticprogressi on?

    SciTech Connect (OSTI)

    Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, MinaJ.

    2007-02-24

    Epithelial-mesenchymal transition (EMT) is a cellular transdifferentiation program that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. However, a similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, in which it is associated with disease progression. EMT in cancer epithelial cells often appears to be an incomplete and bi-directional process. Here we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the Ras-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  3. Discussion on a Code Comparison Effort for the Geothermal Technologies

    Office of Environmental Management (EM)

    About Energy.gov » Directory Listings Directory Listings There are three DOE Directory Listings: An alphabetical listing of all DOE federal employees including their telephone number, routing symbol, and location (building/room number) A listing of Headquarters staff by organization including their telephone number, routing symbol, location (building/room number), and fax number (when available) A listing of Field staff by organization including their telephone number, location (building/room

  4. Science and Cellular Stresses | U.S. DOE Office of Science (SC...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Science and Cellular Stresses News News Home Featured Articles 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Science Headlines Science Highlights Presentations & ...

  5. TO WHOM IT MAY CONCERN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    TO WHOM IT MAY CONCERN I hereby authorize the release of my occupational radiation exposure records to the Duke University/Duke University Medical Center Radiation Safety Officer. Name (Please print) Signature Duke Unique ID Date Please fax this form back to Radiation Safety at 668-2783. Thank you!  Duke University Duke University Medical Center Durham, North Carolina 27710 Occupational and Environmental Safety Office BOX 3155 RADIATION SAFETY DIVISION TELEPHONE (919) 684-3155 FAX (919)

  6. IGF-I enhances cellular senescence via the reactive oxygen species-p53 pathway

    SciTech Connect (OSTI)

    Handayaningsih, Anastasia-Evi; Takahashi, Michiko; Fukuoka, Hidenori; Iguchi, Genzo; Nishizawa, Hitoshi; Yamamoto, Masaaki; Suda, Kentaro; Takahashi, Yutaka

    2012-08-24

    Highlights: Black-Right-Pointing-Pointer Cellular senescence plays an important role in tumorigenesis and aging process. Black-Right-Pointing-Pointer We demonstrated IGF-I enhanced cellular senescence in primary confluent cells. Black-Right-Pointing-Pointer IGF-I enhanced cellular senescence in the ROS and p53-dependent manner. Black-Right-Pointing-Pointer These results may explain the underlying mechanisms of IGF-I involvement in tumorigenesis and in regulation of aging. -- Abstract: Cellular senescence is characterized by growth arrest, enlarged and flattened cell morphology, the expression of senescence-associated {beta}-galactosidase (SA-{beta}-gal), and by activation of tumor suppressor networks. Insulin-like growth factor-I (IGF-I) plays a critical role in cellular growth, proliferation, tumorigenesis, and regulation of aging. In the present study, we show that IGF-I enhances cellular senescence in mouse, rat, and human primary cells in the confluent state. IGF-I induced expression of a DNA damage marker, {gamma}H2AX, the increased levels of p53 and p21 proteins, and activated SA-{beta}-gal. In the confluent state, an altered downstream signaling of IGF-I receptor was observed. Treatment with a reactive oxygen species (ROS) scavenger, N-acetylcystein (NAC) significantly suppressed induction of these markers, indicating that ROS are involved in the induction of cellular senescence by IGF-I. In p53-null mouse embryonic fibroblasts, the IGF-I-induced augmentation of SA-{beta}-gal and p21 was inhibited, demonstrating that p53 is required for cellular senescence induced by IGF-I. Thus, these data reveal a novel pathway whereby IGF-I enhances cellular senescence in the ROS and p53-dependent manner and may explain the underlying mechanisms of IGF-I involvement in tumorigenesis and in regulation of aging.

  7. Toward Real-time Modeling of Human Heart Ventricles at Cellular...

    Office of Scientific and Technical Information (OSTI)

    Simulation of Drug-induced Arrhythmias Citation Details In-Document Search Title: Toward Real-time Modeling of Human Heart Ventricles at Cellular Resolution: Multi-hour Simulation ...

  8. Build a Network, Cellular Style | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Build a Network, Cellular Style Basic Energy Sciences (BES) BES Home About Research Facilities Science Highlights Benefits of BES Funding Opportunities Basic Energy Sciences Advisory Committee (BESAC) Community Resources Contact Information Basic Energy Sciences U.S. Department of Energy SC-22/Germantown Building 1000 Independence Ave., SW Washington, DC 20585 P: (301) 903-3081 F: (301) 903-6594 E: Email Us More Information » 11.01.15 Build a Network, Cellular Style Bio-based molecular machines

  9. Compression of 1-mm-thick M9763 cellular silicone foam under lateral

    Office of Scientific and Technical Information (OSTI)

    confinement (Technical Report) | SciTech Connect Compression of 1-mm-thick M9763 cellular silicone foam under lateral confinement Citation Details In-Document Search Title: Compression of 1-mm-thick M9763 cellular silicone foam under lateral confinement Abstract not provided. Authors: Small, W. [1] + Show Author Affiliations Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States) Publication Date: 2014-11-13 OSTI Identifier: 1183540 Report Number(s): LLNL-TR--667442 DOE

  10. Cell-to-cell communication and cellular environment alter the somatostatin status of delta cells

    SciTech Connect (OSTI)

    Kelly, Catriona; Flatt, Peter R.; McClenaghan, Neville H.

    2010-08-20

    Research highlights: {yields} TGP52 cells display enhanced functionality in pseudoislet form. {yields} Somatostatin content was reduced, but secretion increased in high glucose conditions. {yields} Cellular interactions and environment alter the somatostatin status of TGP52 cells. -- Abstract: Introduction: Somatostatin, released from pancreatic delta cells, is a potent paracrine inhibitor of insulin and glucagon secretion. Islet cellular interactions and glucose homeostasis are essential to maintain normal patterns of insulin secretion. However, the importance of cell-to-cell communication and cellular environment in the regulation of somatostatin release remains unclear. Methods: This study employed the somatostatin-secreting TGP52 cell line maintained in DMEM:F12 (17.5 mM glucose) or DMEM (25 mM glucose) culture media. The effect of pseudoislet formation and culture medium on somatostatin content and release in response to a variety of stimuli was measured by somatostatin EIA. In addition, the effect of pseudoislet formation on cellular viability (MTT and LDH assays) and proliferation (BrdU ELISA) was determined. Results: TGP52 cells readily formed pseudoislets and showed enhanced functionality in three-dimensional form with increased E-cadherin expression irrespective of the culture environment used. However, culture in DMEM decreased cellular somatostatin content (P < 0.01) and increased somatostatin secretion in response to a variety of stimuli including arginine, calcium and PMA (P < 0.001) when compared with cells grown in DMEM:F12. Configuration of TGP52 cells as pseudoislets reduced the proliferative rate and increased cellular cytotoxicity irrespective of culture medium used. Conclusions: Somatostatin secretion is greatly facilitated by cell-to-cell interactions and E-cadherin expression. Cellular environment and extracellular glucose also significantly influence the function of delta cells.

  11. A coarse-grained model for the simulations of biomolecular interactions in cellular environments

    SciTech Connect (OSTI)

    Xie, Zhong-Ru; Chen, Jiawen; Wu, Yinghao

    2014-02-07

    The interactions of bio-molecules constitute the key steps of cellular functions. However, in vivo binding properties differ significantly from their in vitro measurements due to the heterogeneity of cellular environments. Here we introduce a coarse-grained model based on rigid-body representation to study how factors such as cellular crowding and membrane confinement affect molecular binding. The macroscopic parameters such as the equilibrium constant and the kinetic rate constant are calibrated by adjusting the microscopic coefficients used in the numerical simulations. By changing these model parameters that are experimentally approachable, we are able to study the kinetic and thermodynamic properties of molecular binding, as well as the effects caused by specific cellular environments. We investigate the volumetric effects of crowded intracellular space on bio-molecular diffusion and diffusion-limited reactions. Furthermore, the binding constants of membrane proteins are currently difficult to measure. We provide quantitative estimations about how the binding of membrane proteins deviates from soluble proteins under different degrees of membrane confinements. The simulation results provide biological insights to the functions of membrane receptors on cell surfaces. Overall, our studies establish a connection between the details of molecular interactions and the heterogeneity of cellular environments.

  12. Tuning of the electro-mechanical behavior of the cellular carbon nanotube structures with nanoparticle dispersions

    SciTech Connect (OSTI)

    Gowda, Prarthana; Misra, Abha; Ramamurty, Upadrasta; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah 21589

    2014-03-10

    The mechanical and electrical characteristics of cellular network of the carbon nanotubes (CNT) impregnated with metallic and nonmetallic nanoparticles were examined simultaneously by employing the nanoindentation technique. Experimental results show that the nanoparticle dispersion not only enhances the mechanical strength of the cellular CNT by two orders of magnitude but also imparts variable nonlinear electrical characteristics; the latter depends on the contact resistance between nanoparticles and CNT, which is shown to depend on the applied load while indentation. Impregnation with silver nanoparticles enhances the electrical conductance, the dispersion with copper oxide and zinc oxide nanoparticles reduces the conductance of CNT network. In all cases, a power law behavior with suppression in the differential conductivity at zero bias was noted, indicating electron tunneling through the channels formed at the CNT-nanoparticle interfaces. These results open avenues for designing cellular CNT foams with desired electro-mechanical properties and coupling.

  13. Three-dimensional simulations of cellular non-premixed jet flames

    SciTech Connect (OSTI)

    Valaer, A.L.; Frouzakis, C.E.; Boulouchos, K.; Papas, P.; Tomboulides, A.G.

    2010-04-15

    The formation, dynamics and structure of cellular flames in circular non-premixed jets are examined with three-dimensional numerical simulations incorporating detailed descriptions of chemistry and transport. Similar to past experiments reported in the literature, CO{sub 2}-diluted hydrogen in diluted or pure oxygen co-flowing streams in the proximity of the extinction limit are considered. As in the experiments, several preferred cellular states are found to co-exist with the particular state realized depending on initial conditions as well as on the jet characteristics. The simulations provide additionally the temporal transitions to different stationary or rotating cellular flames, their detailed structure, and the dependence of the scaling of the realized number of cells with the vorticity thickness. (author)

  14. Method of forming a continuous polymeric skin on a cellular foam material

    DOE Patents [OSTI]

    Duchane, David V.; Barthell, Barry L.

    1985-01-01

    Hydrophobic cellular material is coated with a thin hydrophilic polymer skin which stretches tightly over the outer surface of the foam but which does not fill the cells of the foam, thus resulting in a polymer-coated foam structure having a smoothness which was not possible in the prior art. In particular, when the hydrophobic cellular material is a specially chosen hydrophobic polymer foam and is formed into arbitrarily chosen shapes prior to the coating with hydrophilic polymer, inertial confinement fusion (ICF) targets of arbitrary shapes can be produced by subsequently coating the shapes with metal or with any other suitable material. New articles of manufacture are produced, including improved ICF targets, improved integrated circuits, and improved solar reflectors and solar collectors. In the coating method, the cell size of the hydrophobic cellular material, the viscosity of the polymer solution used to coat, and the surface tensin of the polymer solution used to coat are all very important to the coating.

  15. 2012 CELLULAR & MOLECULAR FUNGAL BIOLOGY GORDON RESEARCH CONFERENCE, JUNE 17 - 22, 2012

    SciTech Connect (OSTI)

    Judith Berman

    2012-06-22

    The Gordon Research Conference on CELLULAR & MOLECULAR FUNGAL BIOLOGY was held at Holderness School, Holderness New Hampshire, June 17 - 22, 2012. The 2012 Gordon Conference on Cellular and Molecular Fungal Biology (CMFB) will present the latest, cutting-edge research on the exciting and growing field of molecular and cellular aspects of fungal biology. Topics will range from yeast to filamentous fungi, from model systems to economically important organisms, and from saprophytes and commensals to pathogens of plants and animals. The CMFB conference will feature a wide range of topics including systems biology, cell biology and morphogenesis, organismal interactions, genome organisation and regulation, pathogenesis, energy metabolism, biomass production and population genomics. The Conference was well-attended with 136 participants. Gordon Research Conferences does not permit publication of meeting proceedings.

  16. Piezoelectricity and ferroelectricity of cellular polypropylene electrets films characterized by piezoresponse force microscopy

    SciTech Connect (OSTI)

    Miao, Hongchen; Sun, Yao; Zhou, Xilong; Li, Yingwei; Li, Faxin

    2014-08-14

    Cellular electrets polymer is a new ferroelectret material exhibiting large piezoelectricity and has attracted considerable attentions in researches and industries. Property characterization is very important for this material and current investigations are mostly on macroscopic properties. In this work, we conduct nanoscale piezoelectric and ferroelectric characterizations of cellular polypropylene (PP) films using piezoresponse force microscopy (PFM). First, both the single-frequency PFM and dual-frequency resonance-tracking PFM testings were conducted on the cellular PP film. The localized piezoelectric constant d{sub 33} is estimated to be 7–11pC/N by correcting the resonance magnification with quality factor and it is about one order lower than the macroscopic value. Next, using the switching spectroscopy PFM (SS-PFM), we studied polarization switching behavior of the cellular PP films. Results show that it exhibits the typical ferroelectric-like phase hysteresis loops and butterfly-shaped amplitude loops, which is similar to that of a poly(vinylidene fluoride) (PVDF) ferroelectric polymer film. However, both the phase and amplitude loops of the PP film are intensively asymmetric, which is thought to be caused by the nonzero remnant polarization after poling. Then, the D-E hysteresis loops of both the cellular PP film and PVDF film were measured by using the same wave form as that used in the SS-PFM, and the results show significant differences. Finally, we suggest that the ferroelectric-like behavior of cellular electrets films should be distinguished from that of typical ferroelectrics, both macroscopically and microscopically.

  17. Application of spectral hole burning to the study of in vitro cellular systems

    SciTech Connect (OSTI)

    Milanovich, Nebojsa

    1999-11-08

    Chapter 1 of this thesis describes the various stages of tumor development and a multitude of diagnostic techniques used to detect cancer. Chapter 2 gives an overview of the aspects of hole burning spectroscopy important for its application to the study of cellular systems. Chapter 3 gives general descriptions of cellular organelles, structures, and physical properties that can serve as possible markers for the differentiation of normal and cancerous cells. Also described in Chapter 3 are the principles of cryobiology important for low temperature spectroscopy of cells, characterization of MCF-10F (normal) and MCF-7 (cancer) cells lines which will serve as model systems, and cellular characteristics of aluminum phthalocyanine tetrasulfonate (APT), which was used as the test probe. Chapters 4 and 5 are previously published papers by the author pertaining to the results obtained from the application of hole burning to the study of cellular systems. Chapter 4 presents the first results obtained by spectral hole burning of cellular systems and Chapter 5 gives results for the differentiation of MCF-10F and MCF-7 cells stained with APT by an external applied electric (Stark) field. A general conclusion is presented in Chapter 6. Appendices A and B provide additional characterization of the cell/probe model systems. Appendix A describes the uptake and subcellular distribution of APT in MCF-10F and MCF-7 cells and Appendix B compares the hole burning characteristics of APT in cells when the cells are in suspension and when they are examined while adhering to a glass coverslip. Appendix C presents preliminary results for a novel probe molecule, referred to as a molecular thumbtack, designed by the authors for use in future hole burning applications to cellular systems.

  18. Toward Real-time Modeling of Human Heart Ventricles at Cellular Resolution:

    Office of Scientific and Technical Information (OSTI)

    Multi-hour Simulation of Drug-induced Arrhythmias (Conference) | SciTech Connect Toward Real-time Modeling of Human Heart Ventricles at Cellular Resolution: Multi-hour Simulation of Drug-induced Arrhythmias Citation Details In-Document Search Title: Toward Real-time Modeling of Human Heart Ventricles at Cellular Resolution: Multi-hour Simulation of Drug-induced Arrhythmias Authors: Mirin, A A ; Richards, D F ; Glosli, J N ; Draeger, E W ; Chan, B ; Fattebert, J ; Krauss, W D ; Oppelstrup, T

  19. Telephone. Federal Communications Commission. Marlene H. Dortch...

    Office of Environmental Management (EM)

    ... (GSA), and National Aeronautics and Space Administration (NASA). ACTION: Correction. ... DoD, GSA, and NASA adopted as final, with changes, an interim rule amending the Federal ...

  20. EIS-0298: Telephone Flat Geothermal Development Project

    Broader source: Energy.gov [DOE]

    This EIS is for a Plan of Operation (POO) for Development and Production; and for a POO for Utilization and Disposal for a proposed geothermal development project, including: a power plant, geothermal production and injection wellfield, ancillary facilities, and transmission line on the Modoc National Forest in Siskiyou and Modoc Counties, California.

  1. EIA-877 WINTER HEATING FUELS TELEPHONE SURVEY

    U.S. Energy Information Administration (EIA) Indexed Site

    under the Federal Energy Administration Act of 1974 (Public Law 93-275). Failure to comply may result in criminal fines, civil penalties and other sanctions as provided by law. ...

  2. Telephone. Federal Communications Commission. Marlene H. Dortch...

    Broader source: Energy.gov (indexed) [DOE]

    Commission amends 47 CFR parts 20 and 54 as follows: PART 20-COMMERCIAL MOBILE RADIO SERVICES 1. The authority citation for part 20 continues to read as follows:...

  3. Telephoning for Energy Efficiency in Vermont

    Broader source: Energy.gov [DOE]

    This is a phone call you want to take. NeighborWorks of Western Vermont (NWWVT) is phonebanking homeowners in Shrewsburgh, VT offering up home energy audits at a fraction of the cost to help local residents save energy to save money.

  4. Benjamin Diroll | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Benjamin Diroll Argonne Scholar - Director's Postdoctoral Fellow (Supervisor, Richard Schaller) My research is focused on time-, temperature-, and pressure-resolved spectroscopy of nanoscale materials, especially inorganic colloidal nanocrystals. Telephone 630.252.5418 Fax 630.252.5739 E-mail bdiroll@anl.gov CV/Resume PDF icon Diroll, Ben - CV

  5. Ji-sang Park | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ji-sang Park Postdoctoral Appointee (Supervier, Maria Chan) Research focuses primarily on physical properties of semiconductors for transistor, light emitting diode, and solar cell applications. Telephone 630.252.4628 Fax 630.252.4646 E-mail jisang.park@anl.gov CV/Resume File Park, Ji-Sang - Bio.docx

  6. Kiran Kumar Kovi | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kumar Kovi Postdoctoral Appointee (Supervisor, Ani Sumant) Research is focused on Diamond based electronics with emphasis on high power devices. Other areas of interests include Valleytronics, 2D materials etc. Telephone 630.252.4258 Fax 630.252.5739 E-mail kkovi@anl.gov CV/Resume PDF icon Kiran_Kumar_Kovi

  7. Matthew Sykes | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Matthew Sykes Postdoctoral Appointee (Supervisor, Gary Wiederrecht) Research focuses on characterizing ultrafast processes in metal and semiconductor hybrid nanomaterials for applications in energy conversion. Telephone 630.252.1572 Fax 630.252.4646 E-mail sykes@anl.gov CV/Resume PDF icon Sykes, Matthew - 5-2016 Bio

  8. Leonidas E. Ocola | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Leonidas E. Ocola Physicist Ph.D., University of Wisconsin-Madison Novel nanofabrication techniques, micro- and nanofluidics, plasmonic optical devices, transparent capacitors, templated self-assembly, NEMS, electron-matter interactions, single molecule spectroscopy Telephone 630.252.6613 Fax 630.252.5739 E-mail ocola@anl.gov CV/Resume PDF icon LO resume 4 color

  9. Liang Li | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Liang Li Postdoctoral Appointee (Supervisor, Maria Chan) Current research focuses on ab-initio theoretical studies on hybrid lithium-ion/lithium-oxygen battery materials and photocatalytic reduction of CO2. Telephone 630.252.2788 Fax 630.252.4646 E-mail liangli@anl.gov CV/Resume PDF icon Liang_Li

  10. Dmitri Talapin | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dmitri Talapin Scientist (Joint Appointment) & Professor, Chemistry, University of Chicago Telephone 773.834.2607 Fax 773.702.5863 E-mail dvtalapin@uchicago.edu CV/Resume Microsoft Office document icon Talapin Bio-Revised Jan 2016.doc

  11. Contact

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CAMD Safety Home MSDS Search MSDS Help Safety Training and Tests Contact Links LSU Campus Safety Glossary Name: Email Address Subject: Message: Submit The J. Bennett Johnston, Sr. Center for Advanced Microstructures & Devices 6980 Jefferson Hwy., Baton Rouge, LA 70806 Telephone: 225-578-8887 * Fax: 225-578-6954 Copyright © 2007. All Rights Reserved.

  12. Anand Bhattacharya | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Anand Bhattacharya Physicist Ph.D., University of Minnesota Research focuses on correlated states that emerge in complex oxides at interfaces, in cation-ordered analogs, and due to nanoscale confinement. News Spintronics: spin current from a paramagnet Young scientist discovers magnetic material unnecessary to create spin current Telephone 630.252.6518 Fax 630.252.4646 E-mail anand

  13. Cellular response to low dose radiation: Role of phosphatidylinositol-3 kinase like kinases

    SciTech Connect (OSTI)

    Balajee, A.S.; Meador, J.A.; Su, Y.

    2011-03-24

    It is increasingly realized that human exposure either to an acute low dose or multiple chronic low doses of low LET radiation has the potential to cause different types of cancer. Therefore, the central theme of research for DOE and NASA is focused on understanding the molecular mechanisms and pathways responsible for the cellular response to low dose radiation which would not only improve the accuracy of estimating health risks but also help in the development of predictive assays for low dose radiation risks associated with tissue degeneration and cancer. The working hypothesis for this proposal is that the cellular mechanisms in terms of DNA damage signaling, repair and cell cycle checkpoint regulation are different for low and high doses of low LET radiation and that the mode of action of phosphatidylinositol-3 kinase like kinases (PIKK: ATM, ATR and DNA-PK) determines the dose dependent cellular responses. The hypothesis will be tested at two levels: (I) Evaluation of the role of ATM, ATR and DNA-PK in cellular response to low and high doses of low LET radiation in simple in vitro human cell systems and (II) Determination of radiation responses in complex cell microenvironments such as human EpiDerm tissue constructs. Cellular responses to low and high doses of low LET radiation will be assessed from the view points of DNA damage signaling, DNA double strand break repair and cell cycle checkpoint regulation by analyzing the activities (i.e. post-translational modifications and kinetics of protein-protein interactions) of the key target proteins for PI-3 kinase like kinases both at the intra-cellular and molecular levels. The proteins chosen for this proposal are placed under three categories: (I) sensors/initiators include ATM ser1981, ATR, 53BP1, gamma-H2AX, MDC1, MRE11, Rad50 and Nbs1; (II) signal transducers include Chk1, Chk2, FANCD2 and SMC1; and (III) effectors include p53, CDC25A and CDC25C. The primary goal of this proposal is to elucidate the differences in cellular defense mechanisms between low and high doses of low LET radiation and to define the radiation doses where the cellular DNA damage signaling and repair mechanisms tend to shift. This information is critically important to address and advance some of the low dose research program objectives of DOE. The results of this proposed study will lead to a better understanding of the mechanisms for the cellular responses to low and high doses of low LET radiation. Further, systematic analysis of the role of PIKK signaling pathways as a function of radiation dose in tissue microenvironment will provide useful mechanistic information for improving the accuracy of radiation risk assessment for low doses. Knowledge of radiation responses in tissue microenvironment is important for the accurate prediction of ionizing radiation risks associated with cancer and tissue degeneration in humans.

  14. Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies

    SciTech Connect (OSTI)

    Elmehdawi, Fatima; Wheway, Gabrielle; Szymanska, Katarzyna; Adams, Matthew; High, Alec S.; Johnson, Colin A.; Robinson, Philip A.

    2013-02-01

    HHARI (also known as ARIH1) is an ubiquitin-protein ligase and is the cognate of the E2, UbcH7 (UBE2L3). To establish a functional role for HHARI in cellular proliferation processes, we performed a reverse genetics screen that identified n=86/522 (16.5%) ubiquitin conjugation components that have a statistically significant effect on cell proliferation, which included HHARI as a strong hit. We then produced and validated a panel of specific antibodies that establish HHARI as both a nuclear and cytoplasmic protein that is expressed in all cell types studied. HHARI was expressed at higher levels in nuclei, and co-localized with nuclear bodies including Cajal bodies (p80 coilin, NOPP140), PML and SC35 bodies. We confirmed reduced cellular proliferation after ARIH1 knockdown with individual siRNA duplexes, in addition to significantly increased levels of apoptosis, an increased proportion of cells in G2 phase of the cell cycle, and significant reductions in total cellular RNA levels. In head and neck squamous cell carcinoma biopsies, there are higher levels of HHARI expression associated with increased levels of proliferation, compared to healthy control tissues. We demonstrate that HHARI is associated with cellular proliferation, which may be mediated through its interaction with UbcH7 and modification of proteins in nuclear bodies. -- Highlights: ? We produce and validate new antibody reagents for the ubiquitin-protein ligase HHARI. ? HHARI colocalizes with nuclear bodies including Cajal, PML and SC35 bodies. ? We establish new functions in cell proliferation regulation for HHARI. ? Increased HHARI expression associates with squamous cell carcinoma and proliferation.

  15. Advanced Cellular and Biomolecular Imaging at Lehigh University, (PA) Final Scientific/Technical Report

    SciTech Connect (OSTI)

    Cassimeris, Lynne, U.

    2010-09-10

    Lehigh University is establishing an interdisciplinary program in high resolution cellular and subcellular biological imaging for a range of applications including improved cancer detection. The completed DOE project added to Lehigh?s bio-imaging infrastructure through acquisition of a new confocal microscope system as well as upgrades to two pieces of existing equipment. Bio-imaging related research at Lehigh was also supported through two seed grants for initiation of new projects.

  16. GIM3E: Condition-specific Models of Cellular Metabolism Developed from Metabolomics and Expression Data

    SciTech Connect (OSTI)

    Schmidt, Brian; Ebrahim, Ali; Metz, Thomas O.; Adkins, Joshua N.; Palsson, Bernard O.; Hyduke, Daniel R.

    2013-11-15

    Motivation: Genome-scale metabolic models have been used extensively to investigate alterations in cellular metabolism. The accuracy of these models to represent cellular metabolism in specific conditions has been improved by constraining the model with omics data sources. However, few practical methods for integrating metabolomics data with other omics data sources into genome-scale models of metabolism have been reported. Results: GIMMME (Gene Inactivation Moderated by Metabolism, Metabolomics, and Expression) is an algorithm that enables the development of condition-specific models based on an objective function, transcriptomics, and intracellular metabolomics data. GIMMME establishes metabolite utilization requirements with metabolomics data, uses model-paired transcriptomics data to find experimentally supported solutions, and also provides calculations of the turnover (production / consumption) flux of metabolites. GIMMME was employed to investigate the effects of integrating additional omics datasets to create increasingly constrained solution spaces of Salmonella Typhimurium metabolism during growth in both rich and virulence media. This integration proved to be informative and resulted in a requirement of additional active reactions (12 in each case) or metabolites (26 or 29, respectively). The addition of constraints from transcriptomics also impacted the allowed solution space, and the cellular metabolites with turnover fluxes that were necessarily altered by the change in conditions increased from 118 to 271 of 1397. Availability: GIMMME has been implemented in Python and requires a COBRApy 0.2.x. The algorithm and sample data described here are freely available at: http://opencobra.sourceforge.net/

  17. Phenylbutyric acid induces the cellular senescence through an Akt/p21{sup WAF1} signaling pathway

    SciTech Connect (OSTI)

    Kim, Hag Dong; Jang, Chang-Young; Choe, Jeong Min; Department of Biochemistry, Korea University College of Medicine, Seoul 136-705; Korean Institute of Molecular Medicine and Nutrition, Seoul 136-705 ; Sohn, Jeongwon; Korean Institute of Molecular Medicine and Nutrition, Seoul 136-705 ; Kim, Joon

    2012-06-01

    Highlights: Black-Right-Pointing-Pointer Phenylbutyric acid induces cellular senescence. Black-Right-Pointing-Pointer Phenylbutyric acid activates Akt kinase. Black-Right-Pointing-Pointer The knockdown of PERK also can induce cellular senescence. Black-Right-Pointing-Pointer Akt/p21{sup WAF1} pathway activates in PERK knockdown induced cellular senescence. -- Abstract: It has been well known that three sentinel proteins - PERK, ATF6 and IRE1 - initiate the unfolded protein response (UPR) in the presence of misfolded or unfolded proteins in the ER. Recent studies have demonstrated that upregulation of UPR in cancer cells is required to survive and proliferate. Here, we showed that long exposure to 4-phenylbutyric acid (PBA), a chemical chaperone that can reduce retention of unfolded and misfolded proteins in ER, induced cellular senescence in cancer cells such as MCF7 and HT1080. In addition, we found that treatment with PBA activates Akt, which results in p21{sup WAF1} induction. Interestingly, the depletion of PERK but not ATF6 and IRE1 also induces cellular senescence, which was rescued by additional depletion of Akt. This suggests that Akt pathway is downstream of PERK in PBA induced cellular senescence. Taken together, these results show that PBA induces cellular senescence via activation of the Akt/p21{sup WAF1} pathway by PERK inhibition.

  18. Interacting factors and cellular localization of SR protein-specific kinase Dsk1

    SciTech Connect (OSTI)

    Tang, Zhaohua; Luca, Maria; Taggart-Murphy, Laura; Portillio, Jessica; Chang, Cathey; Guven, Ayse; Lin, Ren-Jang; Murray, Johanne; Carr, Antony

    2012-10-01

    Schizosaccharomyces pombe Dsk1 is an SR protein-specific kinase (SRPK), whose homologs have been identified in every eukaryotic organism examined. Although discovered as a mitotic regulator with protein kinase activity toward SR splicing factors, it remains largely unknown about what and how Dsk1 contributes to cell cycle and pre-mRNA splicing. In this study, we investigated the Dsk1 function by determining interacting factors and cellular localization of the kinase. Consistent with its reported functions, we found that pre-mRNA processing and cell cycle factors are prominent among the proteins co-purified with Dsk1. The identification of these factors led us to find Rsd1 as a novel Dsk1 substrate, as well as the involvement of Dsk1 in cellular distribution of poly(A){sup +} RNA. In agreement with its role in nuclear events, we also found that Dsk1 is mainly localized in the nucleus during G{sub 2} phase and at mitosis. Furthermore, we revealed the oscillation of Dsk1 protein in a cell cycle-dependent manner. This paper marks the first comprehensive analysis of in vivo Dsk1-associated proteins in fission yeast. Our results reflect the conserved role of SRPK family in eukaryotic organisms, and provide information about how Dsk1 functions in pre-mRNA processing and cell-division cycle.

  19. Length Scale Correlations of Cellular Microstructures in Directionally Solidified Binary System

    SciTech Connect (OSTI)

    Yunxue Shen

    2002-08-01

    In a cellular array, a range of primary spacing is found to be stable under given growth conditions. Since a strong coupling of solute field exists between the neighboring cells, primary spacing variation should also influence other microstructure features such as cell shape and cell length. The existence of multiple solutions is examined in this study both theoretically as well as experimentally. A theoretical model is developed that identifies and relates four important microstructural lengths, which are found to be primary spacing, tip radius, cell width and cell length. This general microstructural relationship is shown to be valid for different cells in an array as well as for other cellular patterns obtained under different growth conditions. The unique feature of the model is that the microstructure correlation does not depend on composition or growth conditions since these variables scale microstructural lengths to satisfy the relationship obtained in this study. Detailed directional solidification experimental studies have been carried out in the succinonitrile-salol system to characterize and measure these four length scales. Besides the validation of the model, experimental results showed additional scaling laws to be present. In the regime where only a cellular structure is formed, the shape of the cell, the cell tip radius and the length of the cell are all found to scale individually with the local primary spacing. The presence of multiple solutions of primary spacing is also shown to influence the cell-dendrite transition that is controlled not only by the processing variables (growth velocity, thermal gradient and composition) but also by the local cell spacing. The cell-dendrite transition was found not to be sharp, but occurred over a range of processing conditions. Two critical conditions have been identified such that only cells are present below lower critics condition, and only dendrites are formed above the upper critics condition. Between these two limits, both cells and dendrites have been found to coexist. In this mixed regime, a critical local spacing is found above which a cell is unstable and forms a dendrite. An analytical expression is developed that relates the critical spacing for the cell-dendrite transition with processing conditions.

  20. Length Scale Correlations of Cellular Microstructures in Directionally Solidified Binary System

    SciTech Connect (OSTI)

    Yunxue Shen

    2002-06-27

    In a cellular array, a range of primary spacing is found to be stable under given growth conditions. Since a strong coupling of solute field exists between the neighboring cells, primary spacing variation should also influence other microstructure features such as cell shape and cell length. The existence of multiple solutions is examined in this study both theoretically as well as experimentally. A theoretical model is developed that identifies and relates four important microstructural lengths, which are found to be primary spacing, tip radius, cell width and cell length. This general microstructural relationship is shown to be valid for different cells in an array as well as for other cellular patterns obtained under different growth conditions. The unique feature of the model is that the microstructure correlation does not depend on composition or growth conditions since these variables scale microstructural lengths to satisfy the relationship obtained in this study. Detailed directional solidification experimental studies have been carried out in the succinonitrile-salol system to characterize and measure these four length scales. Besides the validation of the model, experimental results showed additional scaling laws to be present. In the regime where only a cellular structure is formed, the shape of the cell, the cell tip radius and the length of the cell are all found to scale individually with the local primary spacing. The presence of multiple solutions of primary spacing is also shown to influence the cell-dendrite transition that is controlled not only by the processing variables (growth velocity, thermal gradient and composition) but also by the local cell spacing. The cell-dendrite transition was found not to be sharp, but occurred over a range of processing conditions. Two critical conditions have been identified such that only cells are present below lower critics condition, and only dendrites are formed above the upper critics condition. Between these two limits, both cells and dendrites have been found to coexist. In this mixed regime, a critical local spacing is found above which a cell is unstable and forms a dendrite. An analytical expression is developed that relates the critical spacing for the cell-dendrite transition with processing conditions.

  1. Time dependence of tip morphology during cellular/dendritic arrayed growth

    SciTech Connect (OSTI)

    Song, H.; Tewari, S.N.

    1996-04-01

    Succinonitrile-1.9 wt pct acetone has been directionally solidified in 0.7 x 0.7-cm-square cross section pyrex ampoules in order to observe the cell/dendrite tip morphologies, not influenced by the wall effects, which are present during growth in the generally used thin (about 200 {micro}m) crucibles. The tips do not maintain a steady-state shape, as is generally assumed. Instead, they fluctuate within a shape envelope. The extent of fluctuation increases with decreasing growth speed, as the micro structure changes from the dendritic to cellular. The influence of natural convection has been examined by comparing these morphologies with those grown, without convection, in the thin ampoules.

  2. Structure and biochemical characterization of proliferating cellular nuclear antigen from a parasitic protozoon

    SciTech Connect (OSTI)

    Cardona-Felix, Cesar S.; Lara-Gonzalez, Samuel; Brieba, Luis G.

    2012-02-08

    Proliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and biochemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa.

  3. Developing an Abaqus *HYPERFOAM Model for M9747 (4003047) Cellular Silicone Foam

    SciTech Connect (OSTI)

    Siranosian, Antranik A.; Stevens, R. Robert

    2012-04-26

    This report documents work done to develop an Abaqus *HYPERFOAM hyperelastic model for M9747 (4003047) cellular silicone foam for use in quasi-static analyses at ambient temperature. Experimental data, from acceptance tests for 'Pad A' conducted at the Kansas City Plant (KCP), was used to calibrate the model. The data includes gap (relative displacement) and load measurements from three locations on the pad. Thirteen sets of data, from pads with different serial numbers, were provided. The thirty-nine gap-load curves were extracted from the thirteen supplied Excel spreadsheets and analyzed, and from those thirty-nine one set of data, representing a qualitative mean, was chosen to calibrate the model. The data was converted from gap and load to nominal (engineering) strain and nominal stress in order to implement it in Abaqus. Strain computations required initial pad thickness estimates. An Abaqus model of a right-circular cylinder was used to evaluate and calibrate the *HYPERFOAM model.

  4. 7th International Workshop on Microbeam Probes of Cellular Radiation Response

    SciTech Connect (OSTI)

    Brenner, David J.

    2009-07-21

    The extended abstracts that follow present a summary of the Proceedings of the 7th International Workshop: Microbeam Probes of Cellular Radiation Response, held at Columbia University’s Kellogg Center in New York City on March 15–17, 2006. These International Workshops on Microbeam Probes of Cellular Radiation Response have been held regularly since 1993 (1–5). Since the first workshop, there has been a rapid growth (see Fig. 1) in the number of centers developing microbeams for radiobiological research, and worldwide there are currently about 30 microbeams in operation or under development. Single-cell/single-particle microbeam systems can deliver beams of different ionizing radiations with a spatial resolution of a few micrometers down to a few tenths of a micrometer. Microbeams can be used to addressquestions relating to the effects of low doses of radiation (a single radiation track traversing a cell or group of cells), to probe subcellular targets (e.g. nucleus or cytoplasm), and to address questions regarding the propagation of information about DNA damage (for example, the radiation-induced bystander effect). Much of the recent research using microbeams has been to study low-dose effects and ‘‘non-targeted’’ responses such as bystander effects, genomic instability and adaptive responses. This Workshop provided a forum to assess the current state of microbeam technology and current biological applications and to discuss future directions for development, both technological and biological. Over 100 participants reviewed the current state of microbeam research worldwide and reported on new technological developments in the fields of both physics and biology.

  5. Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)

    SciTech Connect (OSTI)

    Pietsch, Constanze; Bucheli, Thomas D.; Wettstein, Felix E.; Burkhardt-Holm, Patricia

    2011-10-01

    Contamination of animal feed with mycotoxins is a major problem for fish feed mainly due to usage of contaminated ingredients for production and inappropriate storage of feed. The use of cereals for fish food production further increases the risk of a potential contamination. Potential contaminants include the mycotoxin deoxynivalenol (DON) which is synthesized by globally distributed fungi of the genus Fusarium. The toxicity of DON is well recognized in mammals. In this study, we confirm cytotoxic effects of DON in established permanent fish cell lines. We demonstrate that DON is capable of influencing the metabolic activity and cell viability in fish cells as determined by different assays to indicate possible cellular targets of this toxin. Evaluation of cell viability by measurement of membrane integrity, mitochondrial activity and lysosomal function after 24 h of exposure of fish cell lines to DON at a concentration range of 0-3000 ng ml{sup -1} shows a biphasic effect on cells although differences in sensitivity occur. The cell lines derived from rainbow trout are particularly sensitive to DON. The focus of this study lies, furthermore, on the effects of DON at different concentrations on production of reactive oxygen species (ROS) in the different fish cell lines. The results show that DON mainly reduces ROS production in all cell lines that were used. Thus, our comparative investigations reveal that the fish cell lines show distinct species-related endpoint sensitivities that also depend on the type of tissue from which the cells were derived and the severity of exposure. - Highlights: > DON uptake by cells is not extensive. > All fish cell lines are sensitive to DON. > DON is most cytotoxic to rainbow trout cells. > Biphasic cellular responses were frequently observed. > Our results are similar to studies on mammalian cell lines.

  6. On the reversibility of transitions between closed and open cellular convection

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Feingold, G.; Koren, I.; Yamaguchi, T.; Kazil, J.

    2015-02-26

    The two-way transition between closed and open cellular convection is addressed in an idealized cloud resolving modeling framework. A series of cloud resolving simulations shows that the transition between closed and open cellular states is asymmetrical, and characterized by a rapid ("runaway") transition from the closed- to the open-cell state, but slower recovery to the closed-cell state. Given that precipitation initiates the closed-open cell transition, and that the recovery requires a suppression of the precipitation, we apply an ad hoc time-varying drop concentration to initiate and suppress precipitation. We show that the asymmetry in the two-way transition occurs even formore » very rapid drop concentration replenishment. The primary barrier to recovery is the loss in turbulence kinetic energy (TKE) associated with the loss in cloud water (and associated radiative cooling), and the stabilization of the boundary layer during the open-cell period. In transitioning from the open to the closed state, the system faces the Sisyphusian task of replenishing cloud water fast enough to counter precipitation losses, such that it can generate radiative cooling and TKE. Recovery to the closed cell state is slower when radiative cooling is inefficient such as in the presence of free tropospheric clouds, or after sunrise, when it is hampered by the absorption of shortwave radiation. Tests suggest that a faster return to the closed-cell state requires that the drop concentration recovery be accompanied by significant dynamical forcing, e.g., via an increase in surface latent and sensible heat fluxes. This is supported by simulations with a simple predator-prey dynamical system analogue. It is suggested that the observed closing of open cells by ship effluent likely occurs when aerosol intrusions are large, when contact comes prior to the heaviest drizzle in the early morning hours, and when the free troposphere is cloud-free.« less

  7. Resveratrol induces cellular senescence with attenuated mono-ubiquitination of histone H2B in glioma cells

    SciTech Connect (OSTI)

    Gao, Zhen; Xu, Michael S.; Barnett, Tamara L.; Xu, C. Wilson

    2011-04-08

    Research highlights: {yields} Resveratrol induces cellular senescence in glioma cell. {yields} Resveratrol inhibits mono-ubiquitination of histone H2B at K120. {yields} Depletion of RNF20, phenocopies the inhibitory effects of resveratrol. {yields} Mono-ubiquitination of histone H2B at K120 is a novel target of resveratrol. {yields} RNF20 inhibits cellular senescence in proliferating glioma cells. -- Abstract: Resveratrol (3,4',5-trihydroxy-trans-stilbene), a polyphenol naturally occurring in grapes and other plants, has cancer chemo-preventive effects and therapeutic potential. Although resveratrol modulates multiple pathways in tumor cells, how resveratrol or its affected pathways converge on chromatin to mediate its effects is not known. Using glioma cells as a model, we showed here that resveratrol inhibited cell proliferation and induced cellular hypertrophy by transforming spindle-shaped cells to enlarged, irregular and flatten-shaped ones. We further showed that resveratrol-induced hypertrophic cells expressed senescence-associated-{beta}-galactosidase, suggesting that resveratrol-induced cellular senescence in glioma cells. Consistent with these observations, we demonstrated that resveratrol inhibited clonogenic efficiencies in vitro and tumor growth in a xenograft model. Furthermore, we found that acute treatment of resveratrol inhibited mono-ubiquitination of histone H2B at K120 (uH2B) in breast, prostate, pancreatic, lung, brain tumor cells as well as primary human cells. Chronic treatment with low doses of resveratrol also inhibited uH2B in the resveratrol-induced senescent glioma cells. Moreover, we showed that depletion of RNF20, a ubiquitin ligase of histone H2B, inhibited uH2B and induced cellular senescence in glioma cells in vitro, thereby recapitulated the effects of resveratrol. Taken together, our results suggest that uH2B is a novel direct or indirect chromatin target of resveratrol and RNF20 plays an important role in inhibiting cellular senescence programs that are intact in glioma cells.

  8. Center for Advanced Solar Photophysics | Contacts

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contacts Victor Klimov - Center for Advnaced Solar Photoohysics Director Center Director Victor I. Klimov C-PCS, MS-J567, Chemistry Division Los Alamos National Laboratory Los Alamos, NM 87545 Office: (505) 665-8284 Cellular: (505) 699-7541 Fax: (505) 667-0440 E-mail: klimov@lanl.gov Artthur Nozik - Center for Advanced Solar Photophysics Associate Center Director Dr. Matthew C. Nozik Senior Scientist National Renewable Energy Laboratory, and University of Colorado, Boulder Ph: 303 384 6603 Fax:

  9. On the Interaction between Marine Boundary Layer Cellular Cloudiness and Surface Heat Fluxes

    SciTech Connect (OSTI)

    Kazil, J.; Feingold, G.; Wang, Hailong; Yamaguchi, T.

    2014-01-02

    The interaction between marine boundary layer cellular cloudiness and surface uxes of sensible and latent heat is investigated. The investigation focuses on the non-precipitating closed-cell state and the precipitating open-cell state at low geostrophic wind speed. The Advanced Research WRF model is used to conduct cloud-system-resolving simulations with interactive surface fluxes of sensible heat, latent heat, and of sea salt aerosol, and with a detailed representation of the interaction between aerosol particles and clouds. The mechanisms responsible for the temporal evolution and spatial distribution of the surface heat fluxes in the closed- and open-cell state are investigated and explained. It is found that the horizontal spatial structure of the closed-cell state determines, by entrainment of dry free tropospheric air, the spatial distribution of surface air temperature and water vapor, and, to a lesser degree, of the surface sensible and latent heat flux. The synchronized dynamics of the the open-cell state drives oscillations in surface air temperature, water vapor, and in the surface fluxes of sensible and latent heat, and of sea salt aerosol. Open-cell cloud formation, cloud optical depth and liquid water path, and cloud and rain water path are identified as good predictors of the spatial distribution of surface air temperature and sensible heat flux, but not of surface water vapor and latent heat flux. It is shown that by enhancing the surface sensible heat flux, the open-cell state creates conditions by which it is maintained. While the open-cell state under consideration is not depleted in aerosol, and is insensitive to variations in sea-salt fluxes, it also enhances the sea-salt flux relative to the closed-cell state. In aerosol-depleted conditions, this enhancement may replenish the aerosol needed for cloud formation, and hence contribute to the perpetuation of the open-cell state as well. Spatial homogenization of the surface fluxes is found to have only a small effect on cloud properties in the investigated cases. This indicates that sub-grid scale spatial variability in the surface flux of sensible and latent heat and of sea salt aerosol may not be required in large scale and global models to describe marine boundary layer cellular cloudiness.

  10. Downregulation of microRNA-498 in colorectal cancers and its cellular effects

    SciTech Connect (OSTI)

    Gopalan, Vinod; Smith, Robert A.; Lam, Alfred K.-Y.

    2015-01-15

    miR-498 is a non-coding RNA located intergenically in 19q13.41. Due to its predicted targeting of several genes involved in control of cellular growth, we examined the expression of miR-498 in colon cancer cell lines and a large cohort of patients with colorectal adenocarcinoma. Two colon cancer cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. The expression of miR-498 was tested in these cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). Tissues from 80 patients with surgical resection of colorectum (60 adenocarcinomas and 20 non-neoplastic tissues) were tested for miR-498 expression by qRT-PCR. In addition, an exogenous miR-498 (mimic) was used to detect the miRNA's effects on cell proliferation and cell cycle events in SW480 using MTT calorimetric assay and flow cytometry respectively. The colon cancer cell lines showed reduced expression of miR-498 compared to a normal colonic epithelial cell line. Mimic driven over expression of miR-498 in the SW480 cell line resulted in reduced cell proliferation and increased proportions of G2-M phase cells. In tissues, miR-498 expression was too low to be detected in all colorectal adenocarcinoma compared to non-neoplastic tissues. This suggests that the down regulation of miR-498 in colorectal cancer tissues and the direct suppressive cellular effect noted in cancer cell lines implies that miR-498 has some direct or indirect role in the pathogenesis of colorectal adenocarcinomas. - Highlights: • miR-498 is a non-coding RNA located in 19q13.41. • Colon cancer cell lines showed reduced expression of miR-498. • Mimic driven over expression of miR-498 in colon cancer cells resulted in lower cell proliferation. • miR-498 expression was down regulated in all colorectal adenocarcinoma tissues.

  11. On the reversibility of transitions between closed and open cellular convection

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Feingold, G.; Koren, I.; Yamaguchi, T.; Kazil, J.

    2015-07-08

    The two-way transition between closed and open cellular convection is addressed in an idealized cloud-resolving modeling framework. A series of cloud-resolving simulations shows that the transition between closed and open cellular states is asymmetrical and characterized by a rapid ("runaway") transition from the closed- to the open-cell state but slower recovery to the closed-cell state. Given that precipitation initiates the closed–open cell transition and that the recovery requires a suppression of the precipitation, we apply an ad hoc time-varying drop concentration to initiate and suppress precipitation. We show that the asymmetry in the two-way transition occurs even for very rapidmore » drop concentration replenishment. The primary barrier to recovery is the loss in turbulence kinetic energy (TKE) associated with the loss in cloud water (and associated radiative cooling) and the vertical stratification of the boundary layer during the open-cell period. In transitioning from the open to the closed state, the system faces the task of replenishing cloud water fast enough to counter precipitation losses, such that it can generate radiative cooling and TKE. It is hampered by a stable layer below cloud base that has to be overcome before water vapor can be transported more efficiently into the cloud layer. Recovery to the closed-cell state is slower when radiative cooling is inefficient such as in the presence of free tropospheric clouds or after sunrise, when it is hampered by the absorption of shortwave radiation. Tests suggest that recovery to the closed-cell state is faster when the drizzle is smaller in amount and of shorter duration, i.e., when the precipitation causes less boundary layer stratification. Cloud-resolving model results on recovery rates are supported by simulations with a simple predator–prey dynamical system analogue. It is suggested that the observed closing of open cells by ship effluent likely occurs when aerosol intrusions are large, when contact comes prior to the heaviest drizzle in the early morning hours, and when the free troposphere is cloud free.« less

  12. Impact of Resolution on Simulation of Closed Mesoscale Cellular Convection Identified by Dynamically Guided Watershed Segmentation

    SciTech Connect (OSTI)

    Martini, Matus; Gustafson, William I.; Yang, Qing; Xiao, Heng

    2014-11-27

    Organized mesoscale cellular convection (MCC) is a common feature of marine stratocumulus that forms in response to a balance between mesoscale dynamics and smaller scale processes such as cloud radiative cooling and microphysics. We use the Weather Research and Forecasting model with chemistry (WRF-Chem) and fully coupled cloud-aerosol interactions to simulate marine low clouds during the VOCALS-REx campaign over the southeast Pacific. A suite of experiments with 3- and 9-km grid spacing indicates resolution-dependent behavior. The simulations with finer grid spacing have smaller liquid water paths and cloud fractions, while cloud tops are higher. The observed diurnal cycle is reasonably well simulated. To isolate organized MCC characteristics we develop a new automated method, which uses a variation of the watershed segmentation technique that combines the detection of cloud boundaries with a test for coincident vertical velocity characteristics. This ensures that the detected cloud fields are dynamically consistent for closed MCC, the most common MCC type over the VOCALS-REx region. We demonstrate that the 3-km simulation is able to reproduce the scaling between horizontal cell size and boundary layer height seen in satellite observations. However, the 9-km simulation is unable to resolve smaller circulations corresponding to shallower boundary layers, instead producing invariant MCC horizontal scale for all simulated boundary layers depths. The results imply that climate models with grid spacing of roughly 3 km or smaller may be needed to properly simulate the MCC structure in the marine stratocumulus regions.

  13. Restriction of Receptor Movement Alters Cellular Response: Physical Force Sensing by EphA2

    SciTech Connect (OSTI)

    Salaita, Khalid; Nair, Pradeep M; Petit, Rebecca S; Neve, Richard M; Das, Debopriya; Gray, Joe W; Groves, Jay T

    2009-09-09

    Activation of the EphA2 receptor tyrosine kinase by ephrin-A1 ligands presented on apposed cell surfaces plays important roles in development and exhibits poorly understood functional alterations in cancer. We reconstituted this intermembrane signaling geometry between live EphA2-expressing human breast cancer cells and supported membranes displaying laterally mobile ephrin-A1. Receptor-ligand binding, clustering, and subsequent lateral transport within this junction were observed. EphA2 transport can be blocked by physical barriers nanofabricated onto the underlying substrate. This physical reorganization of EphA2 alters the cellular response to ephrin-A1, as observed by changes in cytoskeleton morphology and recruitment of a disintegrin and metalloprotease 10. Quantitative analysis of receptor-ligand spatial organization across a library of 26 mammary epithelial cell lines reveals characteristic differences that strongly correlate with invasion potential. These observations reveal a mechanism for spatio-mechanical regulation of EphA2 signaling pathways.

  14. Maintenance of cellular ATP level by caloric restriction correlates chronological survival of budding yeast

    SciTech Connect (OSTI)

    Choi, Joon-Seok; Lee, Cheol-Koo

    2013-09-13

    Highlights: CR decreases total ROS and mitochondrial superoxide during the chronological aging. CR does not affect the levels of oxidative damage on protein and DNA. CR contributes extension of chronological lifespan by maintenance of ATP level -- Abstract: The free radical theory of aging emphasizes cumulative oxidative damage in the genome and intracellular proteins due to reactive oxygen species (ROS), which is a major cause for aging. Caloric restriction (CR) has been known as a representative treatment that prevents aging; however, its mechanism of action remains elusive. Here, we show that CR extends the chronological lifespan (CLS) of budding yeast by maintaining cellular energy levels. CR reduced the generation of total ROS and mitochondrial superoxide; however, CR did not reduce the oxidative damage in proteins and DNA. Subsequently, calorie-restricted yeast had higher mitochondrial membrane potential (MMP), and it sustained consistent ATP levels during the process of chronological aging. Our results suggest that CR extends the survival of the chronologically aged cells by improving the efficiency of energy metabolism for the maintenance of the ATP level rather than reducing the global oxidative damage of proteins and DNA.

  15. Cellular morphology of organic-inorganic hybrid foams based on alkali alumino-silicate matrix

    SciTech Connect (OSTI)

    Verdolotti, Letizia; Capasso, Ilaria; Lavorgna, Marino; Liguori, Barbara; Caputo, Domenico; Iannace, Salvatore

    2014-05-15

    Organic-inorganic hybrid foams based on an alkali alumino-silicate matrix were prepared by using different foaming methods. Initially, the synthesis of an inorganic matrix by using aluminosilicate particles, activated through a sodium silicate solution, was performed at room temperature. Subsequently the viscous paste was foamed by using three different methods. In the first method, gaseous hydrogen produced by the oxidization of Si powder in an alkaline media, was used as blowing agent to generate gas bubbles in the paste. In the second method, the porous structure was generated by mixing the paste with a meringue type of foam previously prepared by whipping, under vigorous stirring, a water solution containing vegetal proteins as surfactants. In the third method, a combination of these two methods was employed. The foamed systems were consolidated for 24 hours at 40C and then characterized by FTIR, X-Ray diffraction, scanning electron microscopy (SEM) and compression tests. Low density foams (?500 Kg/m{sup 3}) with good cellular structure and mechanical properties were obtained by combining the meringue approach with the use of the chemical blowing agent based on Si.

  16. TWO-DIMENSIONAL CELLULAR AUTOMATON MODEL FOR THE EVOLUTION OF ACTIVE REGION CORONAL PLASMAS

    SciTech Connect (OSTI)

    Lpez Fuentes, Marcelo; Klimchuk, James A.

    2015-02-01

    We study a two-dimensional cellular automaton (CA) model for the evolution of coronal loop plasmas. The model is based on the idea that coronal loops are made of elementary magnetic strands that are tangled and stressed by the displacement of their footpoints by photospheric motions. The magnetic stress accumulated between neighbor strands is released in sudden reconnection events or nanoflares that heat the plasma. We combine the CA model with the Enthalpy Based Thermal Evolution of Loops model to compute the response of the plasma to the heating events. Using the known response of the X-Ray Telescope on board Hinode, we also obtain synthetic data. The model obeys easy-to-understand scaling laws relating the output (nanoflare energy, temperature, density, intensity) to the input parameters (field strength, strand length, critical misalignment angle). The nanoflares have a power-law distribution with a universal slope of 2.5, independent of the input parameters. The repetition frequency of nanoflares, expressed in terms of the plasma cooling time, increases with strand length. We discuss the implications of our results for the problem of heating and evolution of active region coronal plasmas.

  17. Direct speciation analysis of arsenic in sub-cellular compartments using micro-X-ray absorption spectroscopy

    SciTech Connect (OSTI)

    Bacquart, Thomas; Deves, Guillaume; Ortega, Richard

    2010-07-15

    Identification of arsenic chemical species at a sub-cellular level is a key to understanding the mechanisms involved in arsenic toxicology and antitumor pharmacology. When performed with a microbeam, X-ray absorption near-edge structure ({mu}-XANES) enables the direct speciation analysis of arsenic in sub-cellular compartments avoiding cell fractionation and other preparation steps that might modify the chemical species. This methodology couples tracking of cellular organelles in a single cell by confocal or epifluorescence microscopy with local analysis of chemical species by {mu}-XANES. Here we report the results obtained with a {mu}-XANES experimental setup based on Kirkpatrick-Baez X-ray focusing optics that maintains high flux of incoming radiation (>10{sup 11} ph/s) at micrometric spatial resolution (1.5x4.0 {mu}m{sup 2}). This original experimental setup enabled the direct speciation analysis of arsenic in sub-cellular organelles with a 10{sup -15} g detection limit. {mu}-XANES shows that inorganic arsenite, As(OH){sub 3}, is the main form of arsenic in the cytosol, nucleus, and mitochondrial network of cultured cancer cells exposed to As{sub 2}O{sub 3}. On the other hand, a predominance of As(III) species is observed in HepG2 cells exposed to As(OH){sub 3} with, in some cases, oxidation to a pentavalent form in nuclear structures of HepG2 cells. The observation of intra-nuclear mixed redox states suggests an inter-individual variability in a cell population that can only be evidenced with direct sub-cellular speciation analysis.

  18. Fact Sheet: Lithium-Ion Batteries for Stationary Energy Storage...

    Energy Savers [EERE]

    batteries offer high energy and power density, making them popular in a variety of mobile applications from cellular telephones to electric vehicles. Li-ion batteries ...

  19. Accelerated cellular senescence phenotype of GAPDH-depleted human lung carcinoma cells

    SciTech Connect (OSTI)

    Phadke, Manali; Krynetskaia, Natalia; Mishra, Anurag; Krynetskiy, Evgeny; Jayne Haines Center for Pharmacogenomics, Temple University School of Pharmacy, Philadelphia, PA 19140

    2011-07-29

    Highlights: {yields} We examined the effect of glyceraldehyde 3-phosphate (GAPDH) depletion on proliferation of human carcinoma A549 cells. {yields} GAPDH depletion induces accelerated senescence in tumor cells via AMPK network, in the absence of DNA damage. {yields} Metabolic and genetic rescue experiments indicate that GAPDH has regulatory functions linking energy metabolism and cell cycle. {yields} Induction of senescence in LKB1-deficient lung cancer cells via GAPDH depletion suggests a novel strategy to control tumor cell proliferation. -- Abstract: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a pivotal glycolytic enzyme, and a signaling molecule which acts at the interface between stress factors and the cellular apoptotic machinery. Earlier, we found that knockdown of GAPDH in human carcinoma cell lines resulted in cell proliferation arrest and chemoresistance to S phase-specific cytotoxic agents. To elucidate the mechanism by which GAPDH depletion arrests cell proliferation, we examined the effect of GAPDH knockdown on human carcinoma cells A549. Our results show that GAPDH-depleted cells establish senescence phenotype, as revealed by proliferation arrest, changes in morphology, SA-{beta}-galactosidase staining, and more than 2-fold up-regulation of senescence-associated genes DEC1 and GLB1. Accelerated senescence following GAPDH depletion results from compromised glycolysis and energy crisis leading to the sustained AMPK activation via phosphorylation of {alpha} subunit at Thr172. Our findings demonstrate that GAPDH depletion switches human tumor cells to senescent phenotype via AMPK network, in the absence of DNA damage. Rescue experiments using metabolic and genetic models confirmed that GAPDH has important regulatory functions linking the energy metabolism and the cell cycle networks. Induction of senescence in LKB1-deficient non-small cell lung cancer cells via GAPDH depletion suggests a novel strategy to control tumor cell proliferation.

  20. Proteomic-based mechanistic investigation of low-dose radiation-induced cellular responses/effects

    SciTech Connect (OSTI)

    Chen, Xian

    2013-10-23

    The goal of our project is to apply our unique systems investigation strategy to reveal the molecular mechanisms underlying the radiation induction and transmission of oxidative damage, adaptive response, and bystander effect at low-doses. Beginning with simple in vitro systems such as fibroblast or epithelial pure culture, our amino acid-coded mass tagging (AACT) comparative proteomic platform will be used to measure quantitatively proteomic changes at high- or low-dose level with respect to their endogenous damage levels respectively, in which a broad range of unique regulated proteins sensitive to low-dose IR will be distinguished. To zoom in how these regulated proteins interact with other in the form of networks in induction/transmission pathways, these regulated proteins will be selected as baits for making a series of fibroblast cell lines that stably express each of them. Using our newly developed method of ?dual-tagging? quantitative proteomics that integrate the capabilities of natural complex expression/formation, simple epitope affinity isolation (not through tandem affinity purification or TAP), and ?in-spectra? AACT quantitative measurements using mass spectrometry (MS), we will be able to distinguish systematically interacting proteins with each bait in real time. Further, in addition to both proteome-wide (global differentially expressed proteins) and pathway-scale (bait-specific) profiling information, we will perform a computational network analysis to elucidate a global pathway/mechanisms underlying cellular responses to real-time low-dose IR. Similarly, we will extend our scheme to investigate systematically those induction/transmission pathways occurring in a fibroblast-epithelial interacting model in which the bystander cell (fibroblast) monitor the IR damage to the target cell (epithelial cell). The results will provide the proteome base (molecular mechanisms/pathways for signaling) for the low dose radiation-induced essential tissue environment interactions, including cell-cell, extracellular matrix and extracellular paracrine interactions.

  1. Improving the accuracy and efficiency of time-resolved electronic spectra calculations: Cellular dephasing representation with a prefactor

    SciTech Connect (OSTI)

    Zambrano, Eduardo; ulc, Miroslav; Van?ek, Ji?

    2013-08-07

    Time-resolved electronic spectra can be obtained as the Fourier transform of a special type of time correlation function known as fidelity amplitude, which, in turn, can be evaluated approximately and efficiently with the dephasing representation. Here we improve both the accuracy of this approximationwith an amplitude correction derived from the phase-space propagatorand its efficiencywith an improved cellular scheme employing inverse Weierstrass transform and optimal scaling of the cell size. We demonstrate the advantages of the new methodology by computing dispersed time-resolved stimulated emission spectra in the harmonic potential, pyrazine, and the NCO molecule. In contrast, we show that in strongly chaotic systems such as the quartic oscillator the original dephasing representation is more appropriate than either the cellular or prefactor-corrected methods.

  2. The role of cellular structure on increasing the detonability limits of three-step chain-branching detonations

    SciTech Connect (OSTI)

    Short, Mark; Kiyanda, Charles B; Quirk, James J; Sharpe, Gary J

    2011-01-27

    In [1], the dynamics of a pulsating three-step chain-branching detonation were studied. The reaction model consists of, sequentially, chain-initiation, chain-branching and chain-termination steps. The chain-initiation and chain-branching steps are taken to be thermally neutral, with chemical energy release occuring in the chain-termination stage. The purpose of the present study is to examine whether cellular detonation structure can increase the value of the chain-branching cross-over temperature T{sub b} at which fully coupled detonation solutions are observed over those in 1 D. The basic concept is straightforward and has been discussed in [1] and [3]; if T{sub s} drops below T{sub b} at the lead shock, the passage of a transverse shock can increase both the lead shock temperature and the temperature behind the transverse wave back above T{sub b}, thus sustaining an unstable cellular detonation for values of T{sub b} for which a one-dimensional pulsating detonation will fail. Experiments potentially supporting this hypothesis with irregular detonations have been shown in [3] in a shock tube with acoustically absorbing walls. Removal of the transverse waves results in detonation failure, giving way to a decoupled shock-flame complex. A number of questions remain to be addressed regarding the possibility of such a mechanism, and, if so, about the precise mechanisms driving the cellular structure for large T{sub b}. For instance, one might ask what sets the cell size in a chain-branching detonation, particularly could the characteristic cell size be set by the chain-branching cross-over temperature T{sub b}: after a transverse wave shock collision, the strength of the transverse wave weakens as it propagates along the front. If the spacing between shock collisions is too large (cell size), then the transverse shocks may weaken to the extent that the lead shock temperature or that behind the transverse waves is not raised above T{sub b}, losing chemical energy to drive the front in those regions. Failure may result if less than sufficient of the lead shock be driven above n to sustain reaction. Our starting point for generating cellular solutions is as in [I], consisting of an initial ZND wave in the channel, but perturbed here by a density non-uniformity to generate a cellular structure. Exactly how far the detonability limits (value of T{sub b}) can be extended is not addressed here, as such issues relate in part to the way the cellular structure is generated [6]. Our concern here is to investigate the mechanisms of self-sustained cellular detonation for values of T{sub b} above those that lead to 1D pulsating wave failure that can be generated from the initial ZND wave. Finally, we do not consider cellular propagation driven by a process of apparent thermal ignition of hot-spots downstream that tends to appear close to the 20 detonability limit. Such events are subject to the lack of correct thermal diffusive physics in the model and thus to the form of numerical dissipation in the underlying flow algorithm.

  3. C_Program FilesRightFaxRFaxGateINA001e2443-0f95-4c73-9ac5-402896144284.TIF

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  4. C_Program FilesRightFaxRFaxGateINA23326efa-6848-4e70-881e-87e980cbdc1a.TIF

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  5. C_Program FilesRightFaxRFaxGateINAa402975d-cd59-4ccc-928d-ecde174f0e37.TIF

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  6. Microsoft Word - DOE Comment Letter.FINAL.doc

    Energy Savers [EERE]

    / 6 GEORGETOWN UNIVERSITY LAW CENTER INSTITUTE FOR PUBLIC REPRESENTATION 600 New Jersey Avenue, NW, Suite 312 Washington, DC 20001-2075 Telephone: 202-662-9535 TDD: 202-662-9538 Fax: 202-662-9634 January 8, 2006 Anthony J. Como SEA Document Manager U.S. Department of Energy 1000 Independence Avenue, SW Washington, DC 20585 Re: Comments on Department of Energy's Special Environmental Analysis Regarding Operation of the Potomac River Generating Station in Alexandria, VA Dear Mr. Como: The

  7. CERTIFIED REALTY SPECIALIST

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CERTIFIED REALTY SPECIALIST Biographical Data Name Organization Office Address Telephone Number Fax Number e-mail address Certification Current Certification Level Date Certified Expiration Date Courses Taken For Certification: Course Provider Date Completed Federal Real Property Leasing Federal Real Property Lease Law Techniques of Negotiation of Federal Property Leases Cost and Price Analysis of Lease Proposals Lease Administration Real Estate Management and Disposal Real Estate Acquisition

  8. Vehicle Technologies Office: Organization and Contacts | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Organization and Contacts Vehicle Technologies Office: Organization and Contacts Vehicle Technologies Office: Organization and Contacts General Information Vehicle Technologies Office Telephone: (202) 586-8055 Fax: (202) 586-7409 Mailing Address: EE-3V Room 5G-030 1000 Independence Ave, SW Washington, DC 20585 Program Contacts Acting Director Christy Cooper christy.cooper@ee.doe.gov Operations Supervisor Christy Cooper christy.cooper@ee.doe.gov Analysis Jacob Ward

  9. Internal Resources

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Drawings/Photos | Links Disclaimer The statements and opinions included in the CAMD SAXS Beamline pages are those of CAMD SAXS Beamline personnel and SAXS web designer only. Any statements and opinions included in these pages are not those of Louisiana State University or the LSU Board of Supervisors. Small Angle X-ray Scattering Beamline at The J. Bennett Johnston, Sr. Center for Advanced Microstructures & Devices Louisiana State University | Telephone: 225-578-8887 | Fax: 225-578-6954

  10. Final Report Appendices: Preliminary Process and Market Evaluation: Better Buildings Neighborhood Program

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Appendices Preliminary Process and Market Evaluation: Better Buildings Neighborhood Program Funded By: Prepared By: December 28, 2012 PRELIMINARY PROCESS AND MARKET EVALUATION: BETTER BUILDINGS NEIGHBORHOOD PROGRAM RESEARCH INTO ACTION, INC. PO BOX 12312 PORTLAND OR, 97212 WWW.RESEARCHINTOACTION.COM DELIVERY: 3934 NE MARTIN LUTHER KING JR. BLVD., SUITE 300 PORTLAND, OR 97212 (DELIVERY) TELEPHONE: 503.287.9136 FAX: 503.281.7375 CONTACT: JANE S. PETERS, PRESIDENT JANEP@RESEARCHINTOACTION.COM

  11. Final Report: Preliminary Process and Market Evaluation: Better Buildings Neighborhood Program

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Preliminary Process and Market Evaluation: Better Buildings Neighborhood Program Funded By: Prepared By: December 28, 2012 PRELIMINARY PROCESS AND MARKET EVALUATION: BETTER BUILDINGS NEIGHBORHOOD PROGRAM RESEARCH INTO ACTION, INC. PO BOX 12312 PORTLAND OR, 97212 WWW.RESEARCHINTOACTION.COM DELIVERY: 3934 NE MARTIN LUTHER KING JR. BLVD., SUITE 300 PORTLAND, OR 97212 (DELIVERY) TELEPHONE: 503.287.9136 FAX: 503.281.7375 CONTACT: JANE S. PETERS, PRESIDENT JANEP@RESEARCHINTOACTION.COM PRELIMINARY

  12. Notices Disabled, 1401 S. Clark Street, Suite

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    5913 Federal Register / Vol. 78, No. 240 / Friday, December 13, 2013 / Notices Disabled, 1401 S. Clark Street, Suite 10800, Arlington, Virginia 22202-4149. FOR FURTHER INFORMATION CONTACT: Barry S. Lineback, Telephone: (703) 603-7740, Fax: (703) 603-0655, or email CMTEFedReg@AbilityOne.gov. SUPPLEMENTARY INFORMATION: Addition On 6/28/2013 (78 FR 38952-38953), the Committee for Purchase From People Who Are Blind or Severely Disabled published notice of proposed addition to the Procurement List.

  13. L.45 EXHIBIT E PAST PERFORMANCE REFERENCE INFORMATION FORM

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    L.45 EXHIBIT E PAST PERFORMANCE REFERENCE INFORMATION FORM 1. Complete name of Government agency, commercial firm, or other organization 2. Complete address 3. Contract number or other reference and type 4. Date of contract 5. Date work commenced 6. Date work was completed or scheduled to be completed 7. Contract Type and Contract Value 8. Final amount invoiced or amount invoiced to date 9a. Technical point of contact (name, title, company/agency, address, telephone number, fax number, and

  14. Microsoft Word - S06970_2010PC

    Office of Legacy Management (LM)

    and Monitoring Report for Corrective Action Unit 417: Central Nevada Test Area Surface, Hot Creek Valley, Nevada October 2010 Approved for public release; further dissemination unlimited LMS/CNT/S06970 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically

  15. Microsoft Word - S07053_PCMP

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    Monitoring and Inspection Plan for Amchitka Island Mud Pit Release Sites, Well Abandonment, and Infantry Road Repair Amchitka, Alaska March 2011 Approved for public release; further dissemination unlimited LMS/AMC/S07053 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx

  16. Richard Schaller | Argonne National Laboratory

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    Richard Schaller Scientist Ph.D., University of California, Berkeley Current research focuses on optical experimental investigations of the electronic structure of quantum confined semiconductor materials, excitonic energy relaxation and dissipation, exciton fine structure, generation and fate of multiple electron-hole pairs, and charge manipulation. News Ratiometric Sensing of Toxins using Quantum Dots New Solar Cell Technology Efficiently Captures High-Energy Photons Telephone 630.252.1423 Fax

  17. Notices

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    8852 Federal Register / Vol. 81, No. 63 / Friday, April 1, 2016 / Notices and Media Programs Division, Office of Compliance, Mail Code 2227A, Environmental Protection Agency, 1200 Pennsylvania Ave. NW., Washington, DC 20460; telephone number: (202) 564-2970; fax number: (202) 564-0050; email address: yellin.patrick@epa.gov. SUPPLEMENTARY INFORMATION: Supporting documents which explain in detail the information that the EPA will be collecting are available in the public docket for this ICR. The

  18. Tapashree Roy | Argonne National Laboratory

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    Tapashree Roy Postdoctoral Appointee (Supervisor, Daniel Lopez) Present research focusses on designing re-configurable nano-photonic devices for high impact applications in science and techonologies. Other interested areas of research include metamaterials, plasmonics, super-resolution microscopy, physics and engineering of super-oscillations and nano-fabrication. Telephone 630.252.4515 Fax 630.252.5739 E-mail troy@anl.gov CV/Resume PDF icon Tapashree_Roy.pdf

  19. SUSANA MARTINEZ Governor JOHN A SANCHEZ Lieutenant Governor

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    February 26, 2016 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www .env.nm.gov CERTIFIED MAIL - RETURN RECEIPT REQUESTED RYAN FLYNN Cabinet Secretary BUTCHTONGATE Deputy Secretary Todd A. Shrader, Manager Carlsbad Field Office Department of Energy Philip J. Breidenbach, Project Manager Nuclear Waste Partnership, LLC P. 0. Box 2078 P. 0. Box 3090 Carlsbad, New Mexico

  20. Request a Quote | The Ames Laboratory

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    Request a Quote Please read our Terms & Conditions before submitting. Title * - Select - Dr Mr Mrs Ms First Name * Last Name * Company/Organization Name * City State Zip Code Country * E-mail Address * Telephone Number * Fax Number To better assist you in fulfilling your material requests please include the flowing details in your request: (1) Mass or quantity required. (2) Composition and basis (i.e. is the request in weight% or atomic%). (3) Any geometrical specifications, or form, such as

  1. 1

    Office of Legacy Management (LM)

    Natural Gas, Produced Water, and Hydrologic Sampling and Analysis Results for 2014 December 2014 Approved for public release; further dissemination unlimited LMS/GSB/S12349 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at

  2. 1

    Office of Legacy Management (LM)

    Monitoring Report Project Shoal Area Subsurface Corrective Action Unit 447 February 2014 Approved for public release; further dissemination unlimited LMS/SHL/S11217 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/

  3. 2014 Well Completion Report for Corrective Action Unit 447 Project Shoal Area Churchill County, Nevada

    Office of Legacy Management (LM)

    Well Completion Report for Corrective Action Unit 447 Project Shoal Area Churchill County, Nevada November 2015 Approved for public release; further dissemination unlimited LMS/SHL/S12297 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at

  4. 2015 Groundwater Monitoring Report Project Shoal Area Subsurface Corrective Action Unit 447

    Office of Legacy Management (LM)

    Groundwater Monitoring Report Project Shoal Area: Subsurface Corrective Action Unit 447 April 2016 Approved for public release; further dissemination unlimited LMS/SHL/S13448 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at

  5. Quick Links | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Quick Facts Quick Facts Quick Facts Financial Data From the SEPA 2014 Annual Report: Total Revenues = $299 million (includes Corps of Engineers' revenues) Total Capital Investment = $2.6 billion (Term of repayment is 50 years from on-line date of each project.) Cumulative Investment Repaid = $938 million Cumulative Interest Paid on Investments = $2.0 billion Contact Information Administrator: Kenneth E. Legg Headquarters: 1166 Athens Tech Road Elberton, GA 30635-6711 Telephone: 706-213-3800 FAX:

  6. Ralu Divan | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ralu Divan Chemist Ph.D., University of Bucharest, Romania Research interests are in the lithographic properties and chemistry of materials, characterizing interfacial and compatibility properties of materials used in MEMS and NEMS, nanogels, and metal nanoparticles synthesis. News Traveling Electrons in Loosely Bound Layers Telephone 630.252.0146 Fax 630.252.5739 E-mail divan@anl.gov CV/Resume PDF icon Dr Divan 2014

  7. Martin Holt | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Martin Holt Scientist Ph.D., University of Illinois at Urbana- Champaign Current research activity focuses on the use of nanoscale X-ray diffraction microscopy as a probe of local structural physics in materials. This is associated with multiple related areas: observation of nanoscale phase phenomena in active materials, observation of unique material behavior of nanoscale objects, and observation of emergent critical dynamics in engineered mesoscale material systems Telephone 630.252.5180 Fax

  8. Michael Sternberg | Argonne National Laboratory

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    Michael Sternberg Senior Scientific Associate Ph.D., University of Paderborn, Germany Research focus is in the integration of various modeling programs, to enable researchers to combine the strengths of each approach to allow solving more complex problems Responsibility for the high-performance computing systems at the center Telephone 630.252.4631 Fax 630.252.4646 E-mail sternberg@anl.gov CV/Resume PDF icon sternberg

  9. DOE F 4220-10

    Energy Savers [EERE]

    20.10 (01-2015) Previous editions are obsolete. U.S. DEPARTMENT OF ENERGY Office of Congressional and Intergovernmental Affairs (CI) CONGRESSIONAL GRANT/CONTRACT NOTIFICATION TO: Office of Congressional & Intergovernmental Affairs ATTN: Contract Notification Coordinator U.S. Department of Energy 1000 Independence Avenue, SW Washington, D. C. 20585 Telephone: 202-586-5450 Fax: 202-586-5497 Email: CI-ANA@hq.doe.gov 1. Procuring Office: Name: (Procurement Office Representative - CS/CO)

  10. Stefan Vajda | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Stefan Vajda Chemist Ph.D., Charles University, Prague Research interest is in the area of physical and chemical properties of size-selected clusters and materials assembled of clusters Adjunct professor, Yale University News Copper clusters capture and convert carbon dioxide to make fuel Telephone 630.252.8123 Fax 630.252.4954 E-mail vajda@anl.gov CV/Resume PDF icon Vajda, Stefan - Bio - 2-9-16

  11. Stephen Gray | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Stephen Gray Group Leader, Theory & Modeling Ph.D., University of California-Berkeley Research activities include the theory and modeling of dynamical processes in nanosystems with particular emphasis on modeling light interactions with metallic nanostructures via rigorous electrodynamics simulations and the quantum dynamics of molecular systems within nanoscale environments. News Shape-shifting nanorods release heat differently Telephone 630.252.3594 Fax 630.252.4646 E-mail gray@anl.gov

  12. Microsoft Word - S05827_WCR_Final.doc

    Office of Legacy Management (LM)

    Well Completion Report for Corrective Action Unit 443 Central Nevada Test Area Nye County, Nevada December 2009 Approved for public release; further dissemination unlimited LMS/CNT/S05827 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at

  13. Microsoft Word - S07896_GW_MR

    Office of Legacy Management (LM)

    11 Groundwater Monitoring Report, Central Nevada Test Area, Subsurface Corrective Action Unit 443 April 2012 Approved for public release; further dissemination unlimited LMS/CNT/S07896 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at

  14. Microsoft Word - S10873_WCR.docx

    Office of Legacy Management (LM)

    3 Well Completion Report for Corrective Action Unit 443 Central Nevada Test Area Nye County, Nevada June 2014 Approved for public release; further dissemination unlimited LMS/CNT/S10873 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at

  15. MSDS Training

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Training Radiation Safety Training Cleanroom Safety Information Test Material Forklift Training Crane Operation Training Tests Radiation Safety Test Forklift Test Crane Operation Test NOTE: All Training and Testing Material is for LSU CAMD Users ONLY! The J. Bennett Johnston, Sr. Center for Advanced Microstructures & Devices 6980 Jefferson Hwy., Baton Rouge, LA 70806 Telephone: 225-578-8887 * Fax: 225-578-6954 Copyright © 2012

  16. Gasoline and Diesel Fuel Update

    Gasoline and Diesel Fuel Update (EIA)

    Price Data Collection Procedures Every Monday, retail on-highway diesel prices are collected by telephone and fax from a sample of approximately 350 retail diesel outlets, including truck stops and service stations. The data represent the price of ultra low sulfur diesel (ULSD) which contains less than 15 parts-per-million sulfur. The Environmental Protection Agency (EPA) requires that all on-highway diesel sold be ULSD by December 1, 2010 (September 1, 2006 in California). In January 2007, the

  17. United States Nuclear Tests July 1945 through September 1992

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    September 2015 United States Nuclear Tests July 1945 through September 1992 U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office This publication supersedes DOE/NV--209, Rev. 15, dated December 2000. This publication has been reproduced directly from the best available copy. Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax:

  18. Valentina Kutepova | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Valentina Kutepova Cleanroom Manager Ph.D., Moscow Tech University, Russia 30 years experience in research and professional engineering development in the field of advanced technologies for thin-film growth and microelectronic devices fabrication and characterization. Numerous innovations in both materials and devices development, and utilization of research results. Telephone 630.252.4290 Fax 630.252.5739 E-mail kutepova@anl.gov CV/Resume PDF icon kutepova.pdf

  19. Agenda080204 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    August 2, 2004 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings August 2, 2004 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting Monday, August 2, 2004 Where: Doubletree Hotel, 1750 Rockville Pike, Rockville, Maryland (Located near the Twinbrook Metro Station on the Red Line) Telephone Number: 301-468-1100 Fax Number:

  20. Agenda10704 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    October 7, 2004 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings October 7, 2004 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting Thursday, October 7, 2004 Where: Quality Suites Hotel, 3 Research Court, Rockville, Maryland (Located near the Shady Grove Metro Station on the Red Line) Telephone Number: 301-840-0200 Fax

  1. Agenda111804 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    18, 2004 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings November 18, 2004 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting Thursday, November 18, 2004 Where: Doubletree Hotel, 1750 Rockville Pike, Rockville, Maryland (Located near the Twinbrook Metro Station on the Red Line) Telephone Number: 301-468-1100 Fax

  2. Agenda31105 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1, 2005 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings March 11, 2005 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting Thursday, March 11, 2005 Where: Doubletree Hotel, 1750 Rockville Pike, Rockville, Maryland (Located near the Twinbrook Metro Station on the Red Line) Telephone Number: 301-468-1100 Fax Number:

  3. Agenda61505 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    15, 2005 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings June 15, 2005 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting Wednesday, June 15, 2005 Where: Marriott Crystal Gateway, 1700 Jefferson Davis Highway, Arlington, VA (Located on the Metro Blue Line) Telephone Number: 703-920-3230 Fax Number: 703-271-5212

  4. SWEAChapter1

    National Nuclear Security Administration (NNSA)

    COVER SHEET RESPONSIBLE AGENCY: National Nuclear Security Administration (NNSA) TITLE: Final Site-Wide Environmental Assessment (SWEA) for Sandia National Laboratories/California (DOE/EA-1422) CONTACT: For further information concerning the Draft Site-Wide Environmental Assessment (SWEA), contact: Susan Lacy, NEPA Document Manager NNSA, Sandia Site Office P.O. Box 5400, Albuquerque, NM 87185 Telephone: 1-505-845-5542, Fax: 505-845-4710 For further information by way of electronic mail, contact:

  5. Anonymous Help Line

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Anonymous Help Line Ombuds Anonymous Help Line Committed to the fair and equitable treatment of all employees, contractors, and persons doing business with the Laboratory. Contact Ombuds Office (505)665-2837 Email Help Line (505) 667-9370 Fax (505) 667-3119 Map & Hours Anonymous Help Line: (505) 667-9370 As an option to visiting the Ombuds office, we provide service through a telephone Help Line and email. The telephone Help Line is useful for individuals who have concerns and want advice or

  6. Shipping and Receiving

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Shipping and Receiving Print On this page: Transport Policy Shipping to the ALS Shipping from the ALS Shipping Hazardous Materials Contacts: ALS Shipping & Receiving (small packages) LBNL Shipping & Receiving (large packages requiring forklift truck) Building 7 Hours: M-F, 7:30 am-4:30 pm Telephone: 510 486 4494 Building 69 Hours: M-F, 7:00 am-3:30 pm Telephone: 510 486 4935 Fax: 510 486 5668 Transport Policy - Getting Your Samples and Equipment to and from the ALS All Lab personnel,

  7. Shipping and Receiving

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Shipping and Receiving Print On this page: Transport Policy Shipping to the ALS Shipping from the ALS Shipping Hazardous Materials Contacts: ALS Shipping & Receiving (small packages) LBNL Shipping & Receiving (large packages requiring forklift truck) Building 7 Hours: M-F, 7:30 am-4:30 pm Telephone: 510 486 4494 Building 69 Hours: M-F, 7:00 am-3:30 pm Telephone: 510 486 4935 Fax: 510 486 5668 Transport Policy - Getting Your Samples and Equipment to and from the ALS All Lab personnel,

  8. Shipping and Receiving

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Shipping and Receiving Print On this page: Transport Policy Shipping to the ALS Shipping from the ALS Shipping Hazardous Materials Contacts: ALS Shipping & Receiving (small packages) LBNL Shipping & Receiving (large packages requiring forklift truck) Building 7 Hours: M-F, 7:30 am-4:30 pm Telephone: 510 486 4494 Building 69 Hours: M-F, 7:00 am-3:30 pm Telephone: 510 486 4935 Fax: 510 486 5668 Transport Policy - Getting Your Samples and Equipment to and from the ALS All Lab personnel,

  9. Contact Information | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contact Information Head of Office of Technology Transfer: Laurie Bagley Princeton Plasma Physics Laboratory P.O. Box 451 Princeton, NJ 08543 Telephone: 609-243-2425 E-mail: lbagley@pppl.gov PPPL Ombudsman: John C. Lacenere Princeton Plasma Physics Laboratory Mail Stop 08 P.O. Box 451 Princeton, NJ 08543 Telephone: 609-243-3308 E-mail: lacenere@pppl.gov Fax: (609) 243-3030 Questions or comments? Please contact Laurie Bagley at lbagley@pppl.gov . Associated Files: PDF icon Patent Awareness

  10. Reconstitution of the cellular response to DNA damage in vitro using damage-activated extracts from mammalian cells

    SciTech Connect (OSTI)

    Roper, Katherine; Coverley, Dawn

    2012-03-10

    In proliferating mammalian cells, DNA damage is detected by sensors that elicit a cellular response which arrests the cell cycle and repairs the damage. As part of the DNA damage response, DNA replication is inhibited and, within seconds, histone H2AX is phosphorylated. Here we describe a cell-free system that reconstitutes the cellular response to DNA double strand breaks using damage-activated cell extracts and naieve nuclei. Using this system the effect of damage signalling on nuclei that do not contain DNA lesions can be studied, thereby uncoupling signalling and repair. Soluble extracts from G1/S phase cells that were treated with etoposide before isolation, or pre-incubated with nuclei from etoposide-treated cells during an in vitro activation reaction, restrain both initiation and elongation of DNA replication in naieve nuclei. At the same time, H2AX is phosphorylated in naieve nuclei in a manner that is dependent upon the phosphatidylinositol 3-kinase-like protein kinases. Notably, phosphorylated H2AX is not focal in naieve nuclei, but is evident throughout the nucleus suggesting that in the absence of DNA lesions the signal is not amplified such that discrete foci can be detected. This system offers a novel screening approach for inhibitors of DNA damage response kinases, which we demonstrate using the inhibitors wortmannin and LY294002. -- Highlights: Black-Right-Pointing-Pointer A cell free system that reconstitutes the response to DNA damage in the absence of DNA lesions. Black-Right-Pointing-Pointer Damage-activated extracts impose the cellular response to DNA damage on naieve nuclei. Black-Right-Pointing-Pointer PIKK-dependent response impacts positively and negatively on two separate fluorescent outputs. Black-Right-Pointing-Pointer Can be used to screen for inhibitors that impact on the response to damage but not on DNA repair. Black-Right-Pointing-Pointer LY294002 and wortmannin demonstrate the system's potential as a pathway focused screening approach.

  11. Investigations of DNA damage induction and repair resulting from cellular exposure to high dose-rate pulsed proton beams

    SciTech Connect (OSTI)

    Renis, M.; Malfa, G.; Tomasello, B.; Borghesi, M.; Schettino, G.; Favetta, M.; Romano, F.; Cirrone, G. A. P.; Manti, L.

    2013-07-26

    Studies regarding the radiobiological effects of low dose radiation, microbeam irradiation services have been developed in the world and today laser acceleration of protons and heavy ions may be used in radiation therapy. The application of different facilities is essential for studying bystander effects and relating signalling phenomena in different cells or tissues. In particular the use of ion beams results advantageous in cancer radiotherapy compared to more commonly used X-rays, since the ability of ions in delivering lethal amount of doses into the target tumour avoiding or limiting damage to the contiguous healthy tissues. At the INFN-LNS in Catania, a multidisciplinary radiobiology group is strategically structured aimed to develop radiobiological research, finalised to therapeutic applications, compatible with the use of high dose laser-driven ion beams. The characteristic non-continuous dose rates with several orders of magnitude of laser-driven ion beams makes this facility very interesting in the cellular systems' response to ultra-high dose rates with non-conventional pulse time intervals cellular studies. Our group have projected to examine the effect of high dose laser-driven ion beams on two cellular types: foetal fibroblasts (normal control cells) and DU145 (prostate cancer cells), studying the modulation of some different bio-molecular parameters, in particular cell proliferation and viability, DNA damage, redox cellular status, morphological alterations of both the cytoskeleton components and some cell organelles and the possible presence of apoptotic or necrotic cell death. Our group performed preliminary experiments with high energy (60 MeV), dose rate of 10 Gy/min, doses of 1, 2, 3 Gy and LET 1 keV/?m on human foetal fibroblasts (control cells). We observed that cell viability was not influenced by the characteristics of the beam, the irradiation conditions or the analysis time. Conversely, DNA damage was present at time 0, immediately following irradiation in a dose-dependent manner. The analysis of repair capability showed that the cells irradiated with 1 and 2 Gy almost completely recovered from the damage, but not, however, 3 Gy treated cells in which DNA damage was not recovered. In addition, the results indicate the importance of the use of an appropriate control in radiobiological in vitro analysis.

  12. Numerical study of mechanical behavior of ceramic composites under compression loading in the framework of movable cellular automaton method

    SciTech Connect (OSTI)

    Konovalenko, Igor S. Smolin, Alexey Yu. Konovalenko, Ivan S.; Promakhov, Vladimir V.; Psakhie, Sergey G.

    2014-11-14

    Movable cellular automaton method was used for investigating the mechanical behavior of ceramic composites under uniaxial compression. A 2D numerical model of ceramic composites based on oxides of zirconium and aluminum with different structural parameters was developed using the SEM images of micro-sections of a real composite. The influence of such structural parameters as the geometrical dimensions of layers, inclusions, and their spatial distribution in the sample, the volume content of the composite components and their mechanical properties (as well as the amount of zirconium dioxide that underwent the phase transformation) on the fracture, strength, deformation and dissipative properties was investigated.

  13. Cellular interactions via conditioned media induce in vivo nephron generation from tubular epithelial cells or mesenchymal stem cells

    SciTech Connect (OSTI)

    Machiguchi, Toshihiko Nakamura, Tatsuo

    2013-06-07

    Highlights: We have attempted in vivo nephron generation using conditioned media. Vascular and tubular cells do cross-talks on cell proliferation and tubular changes. Tubular cells suppress these changes in mesenchymal stem cells. Tubular cells differentiate mesenchymal stem cells into tubular cells. Nephrons can be created from implanted tubular cells or mesenchymal stem cells. -- Abstract: There are some successful reports of kidney generation by utilizing the natural course of kidney development, namely, the use of an artificially treated metanephros, blastocyst or ureteric bud. Under a novel concept of cellular interactions via conditioned media (CMs), we have attempted in vivo nephron generation from tubular epithelial cells (TECs) or mesenchymal stem cells (MSCs). Here we used 10 CMs of vascular endothelial cells (VECs) and TECs, which is the first to introduce a CM into the field of organ regeneration. We first present stimulative cross-talks induced by these CMs between VECs and TECs on cell proliferation and morphological changes. In MSCs, TEC-CM suppressed these changes, however, induced cytokeratin expression, indicating the differentiation of MSCs into TECs. As a result, glomerular and tubular structures were created following the implantation of TECs or MSCs with both CMs. Our findings suggest that the cellular interactions via CMs might induce in vivo nephron generation from TECs or MSCs. As a promoting factor, CMs could also be applied to the regeneration of other organs and tissues.

  14. EIA-877 WINTER HEATING FUELS TELEPHONE SURVEY INSTRUCTIONS

    U.S. Energy Information Administration (EIA) Indexed Site

    ... In such civil action, the court may also issue mandatory injunctions commanding any person to comply with these reporting requirements. 9. FILING FORMS WITH FEDERAL GOVERNMENT AND ...

  15. MASSACHUSETTS AVENUE ChMBRIDGE'39, MASSACHUSETTS TELEPHONE UNrvn...

    Office of Legacy Management (LM)

    UNrvn.,,r, 4-5200 blr. Saul Strauch Technical Liason Division United States Atomic Energy Commission New York Operations Office 70 Columbus Avenue New York 23, New York ...

  16. Alaska Power and Telephone Co | Open Energy Information

    Open Energy Info (EERE)

    Generation Yes Activity Transmission Yes Activity Distribution Yes Activity Wholesale Marketing Yes Activity Retail Marketing Yes This article is a stub. You can help OpenEI by...

  17. GOODS CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone

    Energy Savers [EERE]

    Department of Energy GNEP Element:Demonstrate More Proliferation-Resistant Recycling GNEP Element:Demonstrate More Proliferation-Resistant Recycling An article describing GNEP element of recycling. PDF icon GNEP Element:Demonstrate More Proliferation-Resistant Recycling More Documents & Publications GNEP Element:Develop Enhanced Nuclear Safeguards Global Nuclear Energy Partnership Fact Sheet - Develop Enhanced Nuclear Safeguards Global Nuclear Energy Partnership Fact Sheet - Demonstrate

  18. SBOT CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Related Equipment Merchant Wholesalers 423610 Electrical and Electronic Appliance, Television, and Radio Set Merchant Wholesalers 423620 Other Electronic Parts and Equipment...

  19. SBOT IOWA AMES LAB POC Lisa Rodgers Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Related Equipment Merchant Wholesalers 423610 Electrical and Electronic Appliance, Television, and Radio Set Merchant Wholesalers 423620 Other Electronic Parts and Equipment...

  20. SBOT VIRGINIA THOMAS JEFFERSON LAB POC Danny Llyod Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Packaging Film and Sheet (including Laminated) Manufacturing 326112 Plastics Pipe and Pipe Fitting Manufacturing 326122 Laminated Plastics Plate, Sheet (except Packaging), ...

  1. SBOT NEW JERSEY PRINCETON PLASMA LAB POC Arlene White Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Packaging Film and Sheet (including Laminated) Manufacturing 326112 Plastics Pipe and Pipe Fitting Manufacturing 326122 Laminated Plastics Plate, Sheet (except Packaging), ...

  2. CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen Telephone

    Office of Environmental Management (EM)

    ADMINISTATIVE WASTE REMEDIATION CALIFORNIA LAWRENCE BERKELEY LAB POC David Chen ... Security Systems Services (except Locksmiths) 561621 Hazardous Waste Treatment and ...

  3. SBOT WASHINGTON PACIFIC NORTHWEST LAB POC Kerry T. Bass Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ADMINISTATIVE WASTE REMEDIATION Facilities Support Services 561210 Employment Placement Agencies 561311 Temporary Help Services 561320 Professional Employer Organizations ...

  4. SBOT COLORADO GOLDEN FIELD OFFICE POC Karen Downs Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ADMINISTATIVE WASTE REMEDIATION Facilities Support Services 561210 Employment Placement Agencies 561311 Temporary Help Services 561320 Professional Employer Organizations ...

  5. SBOT MISSOURI KANSAS CITY PLANT POC C. J. Warrick Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Locksmiths 561622 Exterminating and Pest Control Services 561710 Janitorial Services ... Other Fuel Dealers 454319 HEALTH CARE & SOCIAL ASSISTANCE Outpatient Mental Health and ...

  6. SBOT NEVADA NEVADA SITE OFFICE POC Anita Ross Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Locksmiths 561622 Exterminating and Pest Control Services 561710 Janitorial Services ... Business Utilization HEALTH CARE & SOCIAL ASSISTANCE Freestanding Ambulatory ...

  7. SBOT OHIO EM BUSINESS CENTER POC Karen Bahan Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Construction 236220 Water and Sewer Line and Related ... Other Heavy and Civil Engineering Construction 237990 ... Deep Sea Freight Transportation 483111 Inland Water Freight ...

  8. Alaska Power and Telephone Co | Open Energy Information

    Open Energy Info (EERE)

    5,584.29 6,801 2008-06 639 1,752 4,603 1,231 3,853 2,163 1,870 5,605 6,766 2008-05 544.132 1,698 4,529 971.881 3,377 2,148 1,516.013 5,075 6,677 2008-04 646.155 1,969.605 4,471...

  9. SBOT WYOMING ROCKY MOUNTAIN OILFIELD CENTER POC Jenny Krom Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    237110 Oil and Gas Pipeline and Related Structures Construction 237120 Power and Communication Line and Related Structures Construction 237130 Highway, Street, and Bridge ...

  10. SBOT GEORGIA SOUTHEASTERN POWER ADMIN POC Ann Craft Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    236220 Water and Sewer Line and Related Structures Construction 237110 Power and Communication Line and Related Structures Construction 237130 Other Heavy and Civil Engineering ...

  11. Name Name Address Place Zip Category Sector Telephone number...

    Open Energy Info (EERE)

    Hydro Marine and Hydrokinetic http acep uaf edu facilities tanana river hydrokinetic test site aspx Alden Research Laboratory Inc Alden Research Laboratory Inc Shrewsbury Street...

  12. Title 30 Chapter 71 Telegraph, Telephone, and Electric Wires...

    Open Energy Info (EERE)

    and Electric WiresLegal Published NA Year Signed or Took Effect 1969 Legal Citation 30 V.S.A. 2501 et seq. DOI Not Provided Check for DOI availability: http:crossref.org...

  13. SBOT IDAHO IDAHO LAB POC Stacey Francis Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Lessors of Residential Buildings and Dwellings 531110 Other Commercial and Industrial ... Lessors of Residential Buildings and Dwellings 531110 Other Commercial and Industrial ...

  14. SBOT NEW MEXICO CARLSBAD FIELD OFFICE POC Roland Taylor Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Types) Manufacturing 333512 Pump and Pumping Equipment Manufacturing 333911 Air and ... Types) Manufacturing 333512 Pump and Pumping Equipment Manufacturing 333911 Air and ...

  15. SBOT ILLINOIS ARGONNE LAB POC Karl Duke Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Accessory Manufacturing 333515 Pump and Pumping Equipment Manufacturing 333911 Air and ... Types) Manufacturing 333512 Pump and Pumping Equipment Manufacturing 333911 Air and ...

  16. A biphasic endothelial stress-survival mechanism regulates the cellular response to vascular endothelial growth factor A

    SciTech Connect (OSTI)

    Latham, Antony M.; Odell, Adam F.; Mughal, Nadeem A.; Issitt, Theo; Ulyatt, Clare; Walker, John H.; Homer-Vanniasinkam, Shervanthi; Ponnambalam, Sreenivasan

    2012-11-01

    Vascular endothelial growth factor A (VEGF-A) is an essential cytokine that regulates endothelial function and angiogenesis. VEGF-A binding to endothelial receptor tyrosine kinases such as VEGFR1 and VEGFR2 triggers cellular responses including survival, proliferation and new blood vessel sprouting. Increased levels of a soluble VEGFR1 splice variant (sFlt-1) correlate with endothelial dysfunction in pathologies such as pre-eclampsia; however the cellular mechanism(s) underlying the regulation and function of sFlt-1 are unclear. Here, we demonstrate the existence of a biphasic stress response in endothelial cells, using serum deprivation as a model of endothelial dysfunction. The early phase is characterized by a high VEGFR2:sFlt-1 ratio, which is reversed in the late phase. A functional consequence is a short-term increase in VEGF-A-stimulated intracellular signaling. In the late phase, sFlt-1 is secreted and deposited at the extracellular matrix. We hypothesized that under stress, increased endothelial sFlt-1 levels reduce VEGF-A bioavailability: VEGF-A treatment induces sFlt-1 expression at the cell surface and VEGF-A silencing inhibits sFlt-1 anchorage to the extracellular matrix. Treatment with recombinant sFlt-1 inhibits VEGF-A-stimulated in vitro angiogenesis and sFlt-1 silencing enhances this process. In this response, increased VEGFR2 levels are regulated by the phosphatidylinositol-3-kinase and PKB/Akt signaling pathways and increased sFlt-1 levels by the ERK1/2 signaling pathway. We conclude that during serum withdrawal, cellular sensing of environmental stress modulates sFlt-1 and VEGFR2 levels, regulating VEGF-A bioavailability and ensuring cell survival takes precedence over cell proliferation and migration. These findings may underpin an important mechanism contributing to endothelial dysfunction in pathological states. -- Highlights: Black-Right-Pointing-Pointer Endothelial cells mount a stress response under conditions of low serum. Black-Right-Pointing-Pointer Endothelial VEGFR levels are modulated during this response. Black-Right-Pointing-Pointer The cell regulates VEGF-A bioavailability and cell survival. Black-Right-Pointing-Pointer This may partly underlie endothelial dysfunction seen in many pathologies.

  17. Inference of tumor evolution during chemotherapy by computational modeling and in situ analysis of genetic and phenotypic cellular diversity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Almendro, Vanessa; Cheng, Yu -Kang; Randles, Amanda; Itzkovitz, Shalev; Marusyk, Andriy; Ametller, Elisabet; Gonzalez-Farre, Xavier; Muñoz, Montse; Russnes, Hege  G.; Helland, Åslaug; et al

    2014-02-01

    Cancer therapy exerts a strong selection pressure that shapes tumor evolution, yet our knowledge of how tumors change during treatment is limited. Here, we report the analysis of cellular heterogeneity for genetic and phenotypic features and their spatial distribution in breast tumors pre- and post-neoadjuvant chemotherapy. We found that intratumor genetic diversity was tumor-subtype specific, and it did not change during treatment in tumors with partial or no response. However, lower pretreatment genetic diversity was significantly associated with pathologic complete response. In contrast, phenotypic diversity was different between pre- and post-treatment samples. We also observed significant changes in the spatialmore » distribution of cells with distinct genetic and phenotypic features. We used these experimental data to develop a stochastic computational model to infer tumor growth patterns and evolutionary dynamics. Our results highlight the importance of integrated analysis of genotypes and phenotypes of single cells in intact tissues to predict tumor evolution.« less

  18. Inference of tumor evolution during chemotherapy by computational modeling and in situ analysis of genetic and phenotypic cellular diversity

    SciTech Connect (OSTI)

    Almendro, Vanessa; Cheng, Yu -Kang; Randles, Amanda; Itzkovitz, Shalev; Marusyk, Andriy; Ametller, Elisabet; Gonzalez-Farre, Xavier; Muñoz, Montse; Russnes, Hege  G.; Helland, Åslaug; Rye, Inga  H.; Borresen-Dale, Anne -Lise; Maruyama, Reo; van Oudenaarden, Alexander; Dowsett, Mitchell; Jones, Robin  L.; Reis-Filho, Jorge; Gascon, Pere; Gönen, Mithat; Michor, Franziska; Polyak, Kornelia

    2014-02-01

    Cancer therapy exerts a strong selection pressure that shapes tumor evolution, yet our knowledge of how tumors change during treatment is limited. Here, we report the analysis of cellular heterogeneity for genetic and phenotypic features and their spatial distribution in breast tumors pre- and post-neoadjuvant chemotherapy. We found that intratumor genetic diversity was tumor-subtype specific, and it did not change during treatment in tumors with partial or no response. However, lower pretreatment genetic diversity was significantly associated with pathologic complete response. In contrast, phenotypic diversity was different between pre- and post-treatment samples. We also observed significant changes in the spatial distribution of cells with distinct genetic and phenotypic features. We used these experimental data to develop a stochastic computational model to infer tumor growth patterns and evolutionary dynamics. Our results highlight the importance of integrated analysis of genotypes and phenotypes of single cells in intact tissues to predict tumor evolution.

  19. Depletion of cellular poly (A) binding protein prevents protein synthesis and leads to apoptosis in HeLa cells

    SciTech Connect (OSTI)

    Thangima Zannat, Mst.; Bhattacharjee, Rumpa B.; Bag, Jnanankur

    2011-05-13

    Highlights: {yields} Depletion of cellular PABP level arrests mRNA translation in HeLa cells. {yields} PABP knock down leads to apoptotic cell death. {yields} PABP depletion does not affect transcription. {yields} PABP depletion does not lead to nuclear accumulation of mRNA. -- Abstract: The cytoplasmic poly (A) binding protein (PABP) is important in mRNA translation and stability. In yeast, depletion of PABP leads to translation arrest. Similarly, the PABP gene in Drosophila is important for proper development. It is however uncertain, whether mammalian PABP is essential for mRNA translation. Here we showed the effect of PABP depletion on mRNA metabolism in HeLa cells by using a small interfering RNA. Our results suggest that depletion of PABP prevents protein synthesis and consequently leads to cell death through apoptosis. Interestingly, no detectable effect of PABP depletion on transcription, transport and stability of mRNA was observed.

  20. Microbial Protein-Protein Interactions (MiPPI) Data from the Genomics: GTL Center for Molecular and Cellular Systems (CMCS)

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    The Genomic Science Center for Molecular and Cellular Systems (CMCS), established in 2002, seeks to identify and characterize the complete set of protein complexes within a cell to provide a mechanistic basis for the understanding of biochemical functions. The CMCS is anchored at ORNL and PNNL. CMCS initially focused on the identification and characterization of protein complexes in two microbial systems,Rhodopseudomonas palustris (R. palustris) and Shewanella oneidensis (S. oneidensis). These two organisms have also been the focus of major DOE Genomic Science/Microbial Cell Program (MCP) projects. To develop an approach for identifying the diverse types of complexes present in microbial organisms, CMCS incorporates a number of molecular biology, microbiology, analytical and computational tools in an integrated pipeline.

  1. MNK1 expression increases during cellular senescence and modulates the subcellular localization of hnRNP A1

    SciTech Connect (OSTI)

    Ziaei, Samira; The Graduate School and University Center of CUNY, New York, NY ; Shimada, Naoko; Kucharavy, Herman; Hubbard, Karen; The Graduate School and University Center of CUNY, New York, NY

    2012-03-10

    Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is an RNA-binding protein that modulates splice site usage, polyadenylation, and cleavage efficiency. This protein has also been implicated in mRNA stability and transport from the nucleus. We have previously demonstrated that hnRNP A1 had diminished protein levels and showed cytoplasmic accumulation in senescent human diploid fibroblasts. Furthermore, we have shown that inhibition of p38 MAPK, a key regulator of cellular senescence, elevated hnRNP A1 protein levels and inhibited hnRNP A1 cytoplasmic localization. In this study, we have explored the possible involvement of MNK1, one of the downstream effector of p38 MAPK, in the regulation of hnRNP A1. We have demonstrated that pharmacological inhibition of MNK1 by CGP 57380 decreased the phosphorylation levels of hnRNP A1 in young and senescent fibroblast cells and blocked the cytoplasmic accumulation of hnRNP A1 in senescent cells. In addition, MNK1 formed a complex with hnRNP A1 in vivo. The expression levels of MNK1, phospho-MNK1, and phospho-eIF4E proteins were found to be elevated in senescent cells. These data suggest that MNK1 regulates the phosphorylation and the subcellular distribution of hnRNP A1 and that MNK1 may play a role in the induction of senescence. -- Highlights: Black-Right-Pointing-Pointer MNK1 and not MAPKAPK2 phosphorylates hnRNP A1. Black-Right-Pointing-Pointer MNK1 has elevated levels in senescent cells, this has not been reported previously. Black-Right-Pointing-Pointer MNK1 activity induces cytoplasmic accumulation of hnRNP A1 in senescent cells. Black-Right-Pointing-Pointer Altered cytoplasmic localization of hnRNP A1 may alter gene expression patterns. Black-Right-Pointing-Pointer Our studies may increase our understanding of RNA metabolism during cellular aging.

  2. Comparative Iron Oxide Nanoparticle Cellular Dosimetry and Response in Mice by the Inhalation and Liquid Cell Culture Exposure Routes

    SciTech Connect (OSTI)

    Teeguarden, Justin G.; Mikheev, Vladimir B.; Minard, Kevin R.; Forsythe, William C.; Wang, Wei; Sharma, Gaurav; Karin, Norman J.; Tilton, Susan C.; Waters, Katrina M.; Asgharian, Bahman; Price, Owen; Pounds, Joel G.; Thrall, Brian D.

    2014-01-01

    testing the rapidly growing number of nanomaterials requires large scale use of in vitro systems under the presumption that these systems are sufficiently predictive or descriptive of responses in in vivo systems for effective use in hazard ranking. We hypothesized that improved relationships between in vitro and in vivo models of experimental toxicology for nanomaterials would result from placing response data in vitro and in vivo on the same dose scale, the amount of material associated with cells (target cell dose). Methods: Balb/c mice were exposed nose-only to an aerosol of 12.8 nm (68.6 nm CMD, 19.9 mg/m3, 4 hours) super paramagnetic iron oxide particles, target cell doses were calculated and biomarkers of response anchored with histological evidence were identified by global transcriptomics. Representative murine epithelial and macrophage cell types were exposed in vitro to the same material in liquid suspension for four hours and levels nanoparticle regulated cytokine transcripts identified in vivo were quantified as a function of measured nanoparticle cellular dose. Results. Target tissue doses of 0.009-0.4 μg SPIO/cm2 lung led to an inflammatory response in the alveolar region characterized by interstitial inflammation and macrophage infiltration. In vitro, higher target tissue doses of ~1.2-4 μg SPIO/ cm2 of cells were required to induce transcriptional regulation of markers of inflammation, CXCL2 CCL3, in C10 lung epithelial cells. Estimated in vivo macrophage SPIO nanoparticle doses ranged from 1-100 pg/cell, and induction of inflammatory markers was observed in vitro in macrophages at doses of 8-35 pg/cell. Conclusions: Application of target tissue dosimetry revealed good correspondence between target cell doses triggering inflammatory processes in vitro and in vivo in the alveolar macrophage population, but not in the epithelial cells of the alveolar region. These findings demonstrate the potential for target tissue dosimetry to enable the more quantitative comparison of in vitro and in vivo systems advance their use for hazard assessment and extrapolation to humans. The mildly inflammogentic cellular doses experienced by mice were similar those calculated for humans exposed to the same at the existing permissible exposure limit of 10 mg/m3 iron oxide (as Fe).

  3. CD147 and AGR2 expression promote cellular proliferation and metastasis of head and neck squamous cell carcinoma

    SciTech Connect (OSTI)

    Sweeny, Larissa; Liu, Zhiyong; Bush, Benjamin D.; Hartman, Yolanda; Zhou, Tong; Rosenthal, Eben L.

    2012-08-15

    The signaling pathways facilitating metastasis of head and neck squamous cell carcinoma (HNSCC) cells are not fully understood. CD147 is a transmembrane glycoprotein known to induce cell migration and invasion. AGR2 is a secreted peptide also known to promote cell metastasis. Here we describe their importance in the migration and invasion of HNSCC cells (FADU and OSC-19) in vitro and in vivo. In vitro, knockdown of CD147 or AGR2 decreased cellular proliferation, migration and invasion. In vivo, knockdown of CD147 or AGR2 expression decreased primary tumor growth as well as regional and distant metastasis. -- Highlights: Black-Right-Pointing-Pointer We investigated AGR2 in head and neck squamous cell carcinoma for the first time. Black-Right-Pointing-Pointer We explored the relationship between AGR2 and CD147 for the first time. Black-Right-Pointing-Pointer AGR2 and CD147 appear to co-localize in head and squamous cell carcinoma samples. Black-Right-Pointing-Pointer Knockdown of both AGR2 and CD147 reduced migration and invasion in vitro. Black-Right-Pointing-Pointer Knockdown of both AGR2 and CD147 decreased metastasis in vivo.

  4. Development of a cell culture surface conversion technique using alginate thin film for evaluating effect upon cellular differentiation

    SciTech Connect (OSTI)

    Nakashima, Y.; Tsusu, K.; Minami, K.; Nakanishi, Y.

    2014-06-15

    Here, we sought to develop a cell culture surface conversion technique that would not damage living cells. An alginate thin film, formed on a glass plate by spin coating of sodium alginate solution and dipping into calcium chloride solution, was used to inhibit adhesion of cells. The film could be removed by ethylenediaminetetraacetate (EDTA) at any time during cell culture, permitting observation of cellular responses to conversion of the culture surface in real time. Additionally, we demonstrated the validity of the alginate thin film coating method and the performance of the film. The thickness of the alginate thin film was controlled by varying the rotation speed during spin coating. Moreover, the alginate thin film completely inhibited the adhesion of cultured cells to the culture surface, irrespective of the thickness of the film. When the alginate thin film was removed from the culture surface by EDTA, the cultured cells adhered to the culture surface, and their morphology changed. Finally, we achieved effective differentiation of C2C12 myoblasts into myotube cells by cell culture on the convertible culture surface, demonstrating the utility of our novel technique.

  5. Cellular and molecular research to reduce uncertainties in estimates of health effects from low-level radiation

    SciTech Connect (OSTI)

    Elkind, M.M.; Bedford, J.; Benjamin, S.A.; Waldren, C.A. ); Gotchy, R.L. )

    1990-10-01

    A study was undertaken by five radiation scientists to examine the feasibility of reducing the uncertainties in the estimation of risk due to protracted low doses of ionizing radiation. In addressing the question of feasibility, a review was made by the study group: of the cellular, molecular, and mammalian radiation data that are available; of the way in which altered oncogene properties could be involved in the loss of growth control that culminates in tumorigenesis; and of the progress that had been made in the genetic characterizations of several human and animal neoplasms. On the basis of this analysis, the study group concluded that, at the present time, it is feasible to mount a program of radiation research directed at the mechanism(s) of radiation-induced cancer with special reference to risk of neoplasia due to protracted, low doses of sparsely ionizing radiation. To implement a program of research, a review was made of the methods, techniques, and instruments that would be needed. This review was followed by a survey of the laboratories and institutions where scientific personnel and facilities are known to be available. A research agenda of the principal and broad objectives of the program is also discussed. 489 refs., 21 figs., 14 tabs.

  6. Furfuryl alcohol cellular product

    DOE Patents [OSTI]

    Sugama, T.; Kukacka, L.E.

    1982-05-26

    Self-extinguishing rigid foam products are formed by polymerization of furfuryl alcohol in the presence of a lightweight, particulate, filler, zinc chloride and selected catalysts.

  7. No Effect of the Transforming Growth Factor {beta}1 Promoter Polymorphism C-509T on TGFB1 Gene Expression, Protein Secretion, or Cellular Radiosensitivity

    SciTech Connect (OSTI)

    Reuther, Sebastian; Metzke, Elisabeth; Bonin, Michael; Petersen, Cordula; Dikomey, Ekkehard; Raabe, Annette

    2013-02-01

    Purpose: To study whether the promoter polymorphism (C-509T) affects transforming growth factor {beta}1 gene (TGFB1) expression, protein secretion, and/or cellular radiosensitivity for both human lymphocytes and fibroblasts. Methods and Materials: Experiments were performed with lymphocytes taken either from 124 breast cancer patients or 59 pairs of normal monozygotic twins. We used 15 normal human primary fibroblast strains as controls. The C-509T genotype was determined by polymerase chain reaction-restriction fragment length polymorphism or TaqMan single nucleotide polymorphism (SNP) genotyping assay. The cellular radiosensitivity of lymphocytes was measured by G0/1 assay and that of fibroblasts by colony assay. The amount of extracellular TGFB1 protein was determined by enzyme-linked immunosorbent assay, and TGFB1 expression was assessed via microarray analysis or reverse transcription-polymerase chain reaction. Results: The C-509T genotype was found not to be associated with cellular radiosensitivity, neither for lymphocytes (breast cancer patients, P=.811; healthy donors, P=.181) nor for fibroblasts (P=.589). Both TGFB1 expression and TGFB1 protein secretion showed considerable variation, which, however, did not depend on the C-509T genotype (protein secretion: P=.879; gene expression: lymphocytes, P=.134, fibroblasts, P=.605). There was also no general correlation between TGFB1 expression and cellular radiosensitivity (lymphocytes, P=.632; fibroblasts, P=.573). Conclusion: Our data indicate that any association between the SNP C-509T of TGFB1 and risk of normal tissue toxicity cannot be ascribed to a functional consequence of this SNP, either on the level of gene expression, protein secretion, or cellular radiosensitivity.

  8. Cloning, characterization and sub-cellular localization of gamma subunit of T-complex protein-1 (chaperonin) from Leishmania donovani

    SciTech Connect (OSTI)

    Bhaskar,; Kumari, Neeti [Division of Biochemistry, CSIR-Central Drug Research Institute, Chattar Manzil Palace, PO Box 173, Lucknow (India)] [Division of Biochemistry, CSIR-Central Drug Research Institute, Chattar Manzil Palace, PO Box 173, Lucknow (India); Goyal, Neena, E-mail: neenacdri@yahoo.com [Division of Biochemistry, CSIR-Central Drug Research Institute, Chattar Manzil Palace, PO Box 173, Lucknow (India)] [Division of Biochemistry, CSIR-Central Drug Research Institute, Chattar Manzil Palace, PO Box 173, Lucknow (India)

    2012-12-07

    Highlights: Black-Right-Pointing-Pointer The study presents cloning and characterization of TCP1{gamma} gene from L. donovani. Black-Right-Pointing-Pointer TCP1{gamma} is a subunit of T-complex protein-1 (TCP1), a chaperonin class of protein. Black-Right-Pointing-Pointer LdTCP{gamma} exhibited differential expression in different stages of promastigotes. Black-Right-Pointing-Pointer LdTCP{gamma} co-localized with actin, a cytoskeleton protein. Black-Right-Pointing-Pointer The data suggests that this gene may have a role in differentiation/biogenesis. Black-Right-Pointing-Pointer First report on this chapronin in Leishmania. -- Abstract: T-complex protein-1 (TCP1) complex, a chaperonin class of protein, ubiquitous in all genera of life, is involved in intracellular assembly and folding of various proteins. The gamma subunit of TCP1 complex (TCP1{gamma}), plays a pivotal role in the folding and assembly of cytoskeleton protein(s) as an individual or complexed with other subunits. Here, we report for the first time cloning, characterization and expression of the TCP1{gamma} of Leishmania donovani (LdTCP1{gamma}), the causative agent of Indian Kala-azar. Primary sequence analysis of LdTCP1{gamma} revealed the presence of all the characteristic features of TCP1{gamma}. However, leishmanial TCP1{gamma} represents a distinct kinetoplastid group, clustered in a separate branch of the phylogenic tree. LdTCP1{gamma} exhibited differential expression in different stages of promastigotes. The non-dividing stationary phase promastigotes exhibited 2.5-fold less expression of LdTCP1{gamma} as compared to rapidly dividing log phase parasites. The sub-cellular distribution of LdTCP1{gamma} was studied in log phase promastigotes by employing indirect immunofluorescence microscopy. The protein was present not only in cytoplasm but it was also localized in nucleus, peri-nuclear region, flagella, flagellar pocket and apical region. Co-localization of LdTCP1{gamma} with actin suggests that, this gene may have a role in maintaining the structural dynamics of cytoskeleton of parasite.

  9. Investigation of cellular microstructure and enhanced coercivity in sputtered Sm{sub 2}(CoCuFeZr){sub 17} film

    SciTech Connect (OSTI)

    Bhatt, Ranu Schtz, G.; Bhatt, Pramod

    2014-03-14

    We have investigated the effect of annealing temperature on the microstructure and magnetic properties of Sm{sub 2}(CoCuFeZr){sub 17} films prepared using ion beam sputtering at room temperature. The as-deposited film shows randomly oriented polycrystalline grains and exhibits small coercivity (H{sub C}) of 0.04 T at room temperature. Post annealing of these films at 700?C under Ar atmosphere shows significant changes in the microstructure transforming it to the development of cellular growth, concomitant with enhanced coercivity up to 1.3 T. The enhanced coercivity is explained using the domain wall pinning mechanism.

  10. Accumulated SET protein up-regulates and interacts with hnRNPK, increasing its binding to nucleic acids, the Bcl-xS repression, and cellular proliferation

    SciTech Connect (OSTI)

    Almeida, Luciana O.; Garcia, Cristiana B.; Matos-Silva, Flavia A.; Curti, Carlos; Leopoldino, Andria M.

    2014-02-28

    Highlights: hnRNPK is a new target of SET. SET regulates hnRNPK. SET and hnRNPK accumulation promotes tumorigenesis. SET accumulation is a potential model to study genes regulated by SET-hnRNPK. - Abstract: SET and hnRNPK are proteins involved in gene expression and regulation of cellular signaling. We previously demonstrated that SET accumulates in head and neck squamous cell carcinoma (HNSCC); hnRNPK is a prognostic marker in cancer. Here, we postulate that SET and hnRNPK proteins interact to promote tumorigenesis. We performed studies in HEK293 and HNSCC (HN6, HN12, and HN13) cell lines with SET/hnRNPK overexpression and knockdown, respectively. We found that SET and/or hnRNPK protein accumulation increased cellular proliferation. SET accumulation up-regulated hnRNPK mRNA and total/phosphorylated protein, promoted hnRNPK nuclear location, and reduced Bcl-x mRNA levels. SET protein directly interacted with hnRNPK, increasing both its binding to nucleic acids and Bcl-xS repression. We propose that hnRNPK should be a new target of SET and that SEThnRNPK interaction, in turn, has potential implications in cell survival and malignant transformation.

  11. About Us | Department of Energy

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    Us About Us Department of Energy Facilities By E-mail: You can send an email to the Secretary of Energy at The.Secretary@hq.doe.gov By Phone: 202-586-5000 (Main Switchboard) or use the National Phone Directory For Hearing and/or Speech Impaired: Telephone Communication By Fax: 202-586-4403 *By Mail: U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 *Please note: mail may take up to 3 weeks to arrive. As such, we strongly encourage you to use the email address provided

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    inFormation about the CHRIS faith-based approach to addressing minority health disparities in your community, please contact: rose marie Womble, mSSW Health Education Specialist/Project Manager Oak Ridge Institute for Science and Education P.O. Box 117, MS-37 Oak Ridge, TN 37831-0117 E-mail: rose.womble@orise.orau.gov Telephone: 865.576.6256 Fax: 865.241.3851 "The beauty of the CHRIS program is that it's tailored to provide information that addresses a wide array of health issues within a

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    Los Alamos National Laboratory Advanced Simulation and Computing Menu Events Partnerships Help Center Events Partnerships Help Center Videos Advanced Simulation and Computing Program » Help Center Computing Help Center Help hotlines, hours of operation, training, technical assistance, general information Los Alamos National Laboratory Hours: Monday through Friday, 8:00 a.m. - noon, 1:00-5:00 p.m. Mountain time Telephone: (505) 665-4444 option 3 Fax: (505) 665-6333 E-mail: consult@lanl.gov 24

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    » ; * * " \ ) I O O l i n O A O U 3 N 3 9 6 6 L I M d v This publication and other Energy Information Administration (EIA) publications may be purchased from the Superintendent of Documents, U.S. Government Printing Office. Telephone orders may be directed to: Superintendent of Documents U.S. Government Printing Office Main Order Desk (202)512-1800 FAX: (202) 512-2250 8 a.m. to 4:30 p.m., eastern time, M-F All mail orders should be directed to: U.S. Government Printing Office P.O. Box

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    Contact Us Contact Us For information about the Office of Congressional & Intergovernmental Affairs at the Department of Energy; for assistance in locating information on the web site: By Mail: U.S. Department of Energy 1000 Independence Ave., SW Washington, DC 20585 By Phone: 1-202-586-5450 1-800-dial-DOE (1-800-342-5363) By Fax: 202-586-4891 By E-mail: You can send an email to the Webmaster at robert.tuttle@hq.doe.gov For Hearing and/or Speech Impaired: Telephone Communication Public

  16. URANIUM PRODUCERS OF AMERICA 141 EAST PALACE AVENUE, POST OFFICE Box 669, SANTA FE, NEW MEXICO 87504-0669

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    URANIUM PRODUCERS OF AMERICA 141 EAST PALACE AVENUE, POST OFFICE Box 669, SANTA FE, NEW MEXICO 87504-0669 TELEPHONE(505) 982-4611; FAX (505) 988-2987; WWW.URANJUMPRODUCERSAMERJCA.COM David Henderson U.S. Department of Energy Office of Nuclear Energy Mail Stop NE-52 19901 Germantown Rd. Germantown, MD 20874-1290 April 6, 2015 Re: UP A Response to DOE Notice of Issues for Public Comment on "Excess Uranium Management: Effects of DOE Transfers of Excess Uranium on Domestic Uranium Mining,

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    Sampling Plan for the Amchitka, Alaska, Site 2011 Sampling Event March 2011 Approved for public release; further dissemination unlimited LMS/AMC/S05725 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a

  18. SUSANA MARTINEZ Governor JOHN A SANCHEZ Lieutenant Governor

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    3, 2015 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.env.nm.gov CERTIFIED MAIL - RETURN RECEIPT REQUESTED RYAN FLYNN Cabinet Secretary BUTCH TONGATE Deputy Secretary Todd A. Shrader, Manager Carlsbad Field Office Department of Energy Philip J. Breidenbach, Project Manager Nuclear Waste Partnership, LLC P. 0. Box 2078 P. 0. Box 3090 Carlsbad, New Mexico 88221-2078

  19. SUSANA MARTINEZ Governor JOHN A SANCHEZ Lieutenant Governor

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    August 5, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL- RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RE: WIPP NITRATE SALT

  20. SUSANA MARTINEZ Governor JOHN A SANCHEZ Lieutenant Governor

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    September 24, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL - RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RE: DRAFT UNDERGROUND

  1. SUSANA MARTINEZ Governor JOHN A SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    30, 2015 Jose R. Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, New Mexico 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL- RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RYAN FLYNN Cabinet

  2. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8, 2016 State of New Mexico ENnRONMENTDEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.env.nm.gov CERTIFIED MAIL- RETURN RECEIPT REQUESTED RYAN FLYNN Cabinet Secretary BUTCH TONGATE Deputy Secretary Todd A. Shrader, Manager Carlsbad Field Office Department of Energy Philip J. Breidenbach, Project Manager Nuclear Waste Partnership, LLC P. 0 . Box 2078 P. 0 . Box 3090 Carlsbad. New Mexico 88221-2078

  3. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9, 2014 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL * RETURN RECEIPT REQUESTED RYAN FLYNN Cabinet Secretary BUTCH TONGATE Deputy Secretary Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078

  4. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    29, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv .state.nm. us CERTIFIED MAIL- RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RE: AMENDMENT TO REPORTING

  5. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    October 7, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL- RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RE: MODIFICATION TO MAY

  6. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    22, 2016 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www .env.nm.gov CERTIFIED MAIL - RETURN RECEIPT REQUESTED RYAN FLYNN Cabinet Secretary BUTCH TONGATE Deputy Secretary Todd A. Shrader, Manager Carlsbad Field Office Department of Energy Philip J. Breidenbach, Project Manager Nuclear Waste Partnership, LLC P. 0. Box 2078 P. 0. Box 3090 Carlsbad, New Mexico 88221-2078

  7. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    December 2, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL *RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RE: UNDERGROUND DERIVED

  8. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    November 7, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL - RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RYAN FLYNN Cabinet

  9. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL - RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RYAN FLYNN Cabinet Secretary BUTCH

  10. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    September 10, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL - RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RYAN FLYNN Cabinet

  11. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    , 2015 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www .nmenv.state.nm.us CERTIFIED MAIL - RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico 88221-3090 RYAN FLYNN Cabinet Secretary

  12. SUSANA MARTINEZ Governor JOfiN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    JOfiN A. SANCHEZ Lieutenant Governor May 20,2015 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building I 190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL- RETURN RECEIPT REQUESTED RYAN FLYNN Cabinet Secretary BUTCII TONGA TE Deputy Secretary Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box

  13. SUSANA MARTfNEZ Governor JOHN A. SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A. SANCHEZ Lieutenant Governor December 6, 2014 Jose Franco, Manager Carlsbad Field Office Department of Energy P.O. Box 3090 State of New Mexico ENVIRONMENT DEPARTMENT Harold Runnels Building 1190 Saint Francis Drive, PO Box 5469 Santa Fe, NM 87502-5469 Telephone (505) 827-2855 Fax (505) 827-2836 www.nmenv.state.nm.us CERTIFIED MAIL- RETURN RECEIPT REQUESTED Robert L. McQuinn, Project Manager Nuclear Waste Partnership, LLC P.O. Box 2078 Carlsbad, New Mexico 88221-2078 Carlsbad, New Mexico

  14. Federal Technical Capability Panel

    Energy Savers [EERE]

    Updated: April 2015 1 U. S. Department of Energy and National Nuclear Security Administration Federal Technical Capability Panel Organization Name Telephone Fax E-Mail FTCP CHAIR Chair (DOE/NTC) Karen L. Boardman (505) 845-6444 (505) 845-6079 kboardman@ntc.doe.gov FTCP Deputy Dave Chaney (505) 845-4300 (505) 845-4879 david.chaney@nnsa.doe.gov FTCP Technical Standards Mgr. Jeanette Yarrington (301) 903-7030 (301) 903-3445 Jeanette.Yarrington@hq.doe.gov FTCP Program Coordinator Jeannie Lozoya

  15. Environment, Environmental Restoration, and Waste Management Field Organization Directory

    SciTech Connect (OSTI)

    Not Available

    1993-07-01

    This directory was developed by the Office of Environmental Guidance, RCRA/CERCLA Division (EH-231) from an outgrowth of the Departments efforts to identify and establish the regulatory response lead persons in the Field Organizations. The directory was developed for intemal EH-231 use to identify both the DOE and DOE contractor Field Organizations in the Environment, Environmental Restoration and Waste Management areas. The Field Organization directory is divided into three substantive sections: (1) Environment; (2) Environmental Restoration; and (3) Waste Management which are organized to correspond to the management hierarchy at each Field Organization. The information provided includes the facility name and address, individual managers name, and telephone/fax numbers.

  16. Microsoft Word - S07531_PC_2010_032911

    Office of Legacy Management (LM)

    Calendar Year 2010 March 2011 Approved for public release; further dissemination unlimited LMS/SAL/S07531 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy and its

  17. Microsoft Word - S08711_2011AnnRpt

    Office of Legacy Management (LM)

    1 March 2012 Approved for public release; further dissemination unlimited LMS/SAL/S08711 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of Energy Office of

  18. Microsoft Word - S09886_PC

    Office of Legacy Management (LM)

    Calendar Year 2012 March 2013 Approved for public release; further dissemination unlimited LMS/SAL/S09886 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy and its

  19. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Water Sampling at the Salmon, Mississippi, Site March 2014 Approved for public release; further dissemination unlimited LMS/SAL/S00413 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S.

  20. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Salmon, Mississippi, Site June 2015 LMS/SAL/S01014 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of

  1. documenttemplate

    Office of Legacy Management (LM)

    Sampling and Analysis Report Salmon, Mississippi, Site Calendar Year 2013 March 2014 Approved for public release; further dissemination unlimited LMS/SAL/S10376 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available

  2. 1

    Office of Legacy Management (LM)

    3 December 2013 Approved for public release; further dissemination unlimited LMS/GSB/S10841 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy and its contractors,

  3. 1

    Office of Legacy Management (LM)

    Monitoring Report Project Shoal Area Subsurface Corrective Action Unit 447 March 2015 Approved for public release; further dissemination unlimited LMS/SHL/S12315 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available

  4. 2015 Groundwater Monitoring and Inspection Report Gnome-Coach, New Mexico, Site

    Office of Legacy Management (LM)

    Groundwater Monitoring and Inspection Report Gnome-Coach, New Mexico, Site January 2016 Approved for public release; further dissemination unlimited LMS/GNO/S13360 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/

  5. 2015 Long-Term Hydrologic Monitoring Program Sampling and Analysis Results at Rio Blanco, Colorado

    Office of Legacy Management (LM)

    at Rio Blanco, Colorado December 2015 Approved for public release; further dissemination unlimited LMS/RBL/S13678 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of

  6. DOE-LM/XXXX-2007

    Office of Legacy Management (LM)

    Surface Corrective Action Unit 417 at the Central Nevada Test Area, Nevada, Site January 2015 Approved for public release; further dissemination unlimited LMS/CNT/S12300 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/

  7. Microsoft Word - 2009_NESHAP_06152010.docx

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Available electronically at http://www.osti.gov/bridge Available for a processing fee to the U.S. Department of Energy and its contractors, in paper, from: U.S. Department of Energy Office of Scientific and Technical Information P.O. Box 62 Oak Ridge, TN 37831-0062 Telephone: 865-576-8401 Fax: 865-576-5728 E-mail: reports@adonis.osti.gov National Emission Standards for Hazardous Air Pollutants - Radionuclide Emissions Calendar Year 2009 June 2010 Work Performed Under Contract No.

  8. LMS/CNT/S05935

    Office of Legacy Management (LM)

    Monitoring Report Central Nevada Test Area Subsurface Corrective Action Unit 443 May 2014 Approved for public release; further dissemination unlimited LMS/CNT/S11448 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/

  9. Microsoft Word - S05767_PostClosureInspRpt.doc

    Office of Legacy Management (LM)

    Surface, Hot Creek Valley, Nevada October 2009 Approved for public release; further dissemination is unlimited LMS/CNT/S05767 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S.

  10. Microsoft Word - S05869_2009SampleResultsRpt.doc

    Office of Legacy Management (LM)

    Hydrologic and Natural Gas Sampling and Analysis Results for 2009 November 2009 Approved for public release; further dissemination unlimited LMS/GSB/S05869 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a

  11. Microsoft Word - S05935_GW.doc

    Office of Legacy Management (LM)

    Groundwater Monitoring Report Central Nevada Test Area, Corrective Action Unit 443 September 2010 Approved for public release; further dissemination unlimited LMS/CNT/S05935 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge

  12. Microsoft Word - S07076_Results

    Office of Legacy Management (LM)

    Hydrologic and Natural Gas Sampling and Analysis Results for 2010 December 2010 Approved for public release; further dissemination unlimited LMS/GSB/S07076 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a

  13. Microsoft Word - S07114_MonRpt

    Office of Legacy Management (LM)

    Monitoring and Inspection Report Gnome-Coach Site, New Mexico February 2011 Approved for public release; further dissemination unlimited LMS/GNO/S07114 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a

  14. Microsoft Word - S07116_MR

    Office of Legacy Management (LM)

    Central Nevada Test Area, Corrective Action Unit 443 February 2011 Approved for public release; further dissemination unlimited LMS/CNT/S07116 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee

  15. Microsoft Word - S07305_FMP

    Office of Legacy Management (LM)

    Fluid Management Plan Subsurface Corrective Action Unit 447 Shoal, Nevada, Site September 2011 Approved for public release; further dissemination unlimited LMS/SHL/S07305 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge

  16. Microsoft Word - S09337_MonRpt

    Office of Legacy Management (LM)

    Monitoring and Inspection Report Gnome-Coach, New Mexico, Site March 2013 Approved for public release; further dissemination unlimited LMS/GNO/S09337 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a

  17. Microsoft Word - S09647_GW_MR.doc

    Office of Legacy Management (LM)

    Monitoring Report Central Nevada Test Area, Subsurface Corrective Action Unit 443 April 2013 Approved for public release; further dissemination unlimited LMS/CNT/S09647 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge

  18. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Central Nevada Test Area March 2014 Approved for public release; further dissemination unlimited LMS/CNT/S01113 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy

  19. September 2004 Water Sampling

    Office of Legacy Management (LM)

    4 Groundwater Sampling at the Central Nevada Test Area February 2015 LMS/CNT/S01214 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in

  20. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Groundwater, Surface Water, Produced Water, and Natural Gas Sampling at the Gasbuggy, New Mexico, Site October 2014 LMS/GSB/S00614 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S.

  1. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Rio Blanco, Colorado, Site February 2014 LMS/RBL/S00509 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of

  2. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Rio Blanco, Colorado Site October 2013 LMS/RBL/S00513 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of

  3. September 2004 Water Sampling

    Office of Legacy Management (LM)

    and Surface Water Sampling at the Rio Blanco, Colorado, Site October 2014 LMS/RBL/S00514 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in

  4. September 2004 Water Sampling

    Office of Legacy Management (LM)

    and Surface Water Sampling at the Rio Blanco, Colorado, Site October 2015 LMS/RBL/S00515 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in

  5. September 2004 Water Sampling

    Office of Legacy Management (LM)

    5 Produced Water Sampling at the Rulison, Colorado, Site May 2015 LMS/RUL/S00115 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in paper,

  6. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Rulison, Colorado, Site February 2014 LMS/RUL/S00509 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of

  7. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Rulison, Colorado, Site October 2013 LMS/RUL/S00513 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of

  8. September 2004 Water Sampling

    Office of Legacy Management (LM)

    5 Groundwater and Surface Water Sampling at the Rulison, Colorado, Site October 2015 LMS/RUL/S00515 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its

  9. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Natural Gas and Produced Water Sampling at the Rulison, Colorado, Site November 2014 LMS/RUL/S00714 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its

  10. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Produced Water Sampling at the Rulison, Colorado, Site January 2016 LMS/RUL/S00915 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in

  11. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Project Shoal, Nevada, Site December 2013 LMS/SHL/S00513 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/bridge Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of

  12. September 2004 Water Sampling

    Office of Legacy Management (LM)

    Shoal, Nevada, Site July 2014 LMS/SHL/S00514 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/ Available for a processing fee to U.S. Department of Energy and its contractors, in paper, from: U.S. Department of Energy

  13. Gasoline and Diesel Fuel Update

    Gasoline and Diesel Fuel Update (EIA)

    Price Data Collection Procedures Every Monday, cash self-serve on-highway diesel prices (including taxes) are collected from a sample of approximately 400 retail diesel outlets in the continental U.S. The sample includes a combination of truck stops and service stations that sell on-highway diesel fuel. The data represent the price of ultra low sulfur diesel (ULSD) which contains less than 15 parts-per-million sulfur. The prices are collected via telephone, fax, email, or the internet from

  14. NOIJLVaiSINIWaV NOIlVlAldOdNI AOU3N3 Z661

    Gasoline and Diesel Fuel Update (EIA)

    61- NOIJLVaiSINIWaV NOIlVlAldOdNI AOU3N3 Z661 This publication and other Energy Information Administration (EIA) publications may be purchased from the Superintendent of Documents, U.S. Government Printing Office. AH telephone orders should be directed to: U.S. Government Printing Office McPherson Square Bookstore 1510 H Street, N.W. Washington, DC 20005 (202)653-2050 FAX (202)376-5055 9 a.m. to 5 p.m., eastern time, M-F All mail orders should be directed to: Superintendent of Documents U.S.

  15. NOIlVdlSINIWG V NOUVWHOJNI AOH3N SNOUOa

    Gasoline and Diesel Fuel Update (EIA)

    £661. NOIlVdlSINIWG V NOUVWHOJNI AOH3N SNOUOa fOHd )ioo nno AO^H NH £661 This publication and other Energy Information Administration (EIA) publications may be purchased from the Superintendent of Documents, U.S. Government Printing Office. All telephone orders should be directed to: U.S. Government Printing Office Superintendent of Documents McPherson Square Bookstore U.S. Government Printing Office 1510 H Street, N.W. Washington, DC 20402 Washington, DC 20005 (202)783-3238 (202)653-2050 FAX

  16. Microsoft Word - EEI - DOE SG RFI 3 DRAFT _11 1 10_ CLEAN.docx

    Energy Savers [EERE]

    Pennsylvania Avenue, N.W. Washington, D.C. 20004-2696 Telephone 202-508-5615 Fax 202-508-5673 www.eei.org November 1, 2010 U.S. Department of Energy Office of Electric Delivery and Energy Reliability ATTN: Smart Grid RFI: Addressing Policy and Logistical Challenges 1000 Independence Avenue, SW Room 8H033 Washington, DC 20585 RE: SMART GRID RFI: ADDRESSING POLICY AND LOGISTICAL CHALLENGES TO SMART GRID IMPLEMENTATION The Edison Electric Institute ("EEI"), on behalf of its member

  17. DOE/EIA-0202(92/1Q)

    Gasoline and Diesel Fuel Update (EIA)

    1Q) 1992 1 QUARTER SHORT-TERM ENERGY OUTLOOK QUARTERLY PROJECTIONS ENERGY INFORMATION ADMINISTRATION February 1992 This publication and other Energy Information Administration (EIA) publications may be pur chased from the Superintendent of Documents, U.S. Government Printing Office. All telephone orders should be directed to: U.S. Government Printing Office Farragut Bookstore 1510 H Street N.W. Washington, DC 20005 (202) 653-7697 FAX (202) 376-5055 9 a.m. to 5 p.m., eastern time, M-F

  18. DOE/EIA-0202(92/2Q)

    Gasoline and Diesel Fuel Update (EIA)

    2Q) 1992 2 QUARTER SHORT-TERM ENERGY OUTLOOK QUARTERLY PROJECTIONS May 1992 This publication and other Energy Information Administration (E1A) publications may be pur chased from the Superintendent of Documents, U.S. Government Printing Office. All telephone orders should be directed to: U.S. Government Printing Office Superintendent of Documents Farragut Bookstore U.S. Government Printing Office 151 OH Street N.W. Washington, DC 20402 Washington, DC 20005 (202)783-3238 (202)653-7697 FAX

  19. documenttemplate

    Office of Legacy Management (LM)

    Closure Report Central Nevada Test Area Subsurface Corrective Action Unit 443 January 2016 Approved for public release; further dissemination unlimited LMS/CNT/S12760 Available for sale to the public from: U.S. Department of Commerce National Technical Information Service 5301 Shawnee Road Alexandria, VA 22312 Telephone: 800.553.6847 Fax: 703.605.6900 E-mail: orders@ntis.gov Online Ordering: http://www.ntis.gov/help/ordermethods.aspx Available electronically at http://www.osti.gov/scitech/

  20. Microsoft Word - EERE PSRP 7 23 2010

    Office of Environmental Management (EM)

    Pennsylvania Avenue, N.W. Washington, D.C. 20004-2696 Telephone 202-508-5615 Fax 202-508-5673 www.eei.org November 1, 2010 U.S. Department of Energy Office of Electric Delivery and Energy Reliability ATTN: Smart Grid RFI: Addressing Policy and Logistical Challenges 1000 Independence Avenue, SW Room 8H033 Washington, DC 20585 RE: SMART GRID RFI: ADDRESSING POLICY AND LOGISTICAL CHALLENGES TO SMART GRID IMPLEMENTATION The Edison Electric Institute ("EEI"), on behalf of its member

  1. Agenda030206 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2-3, 2006 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings March 2-3, 2006 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting March 2-3, 2006 Where: Doubletree Hotel, 1750 Rockville Pike, Rockville, Maryland (Located near the Twinbrook Metro Station on the Red Line) Telephone Number: 301-468-1100 Fax Number:

  2. Agenda072106 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    July 21, 2006 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings July 21, 2006 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting July 21, 2006 Where: Bethesda North Marriott and Conference Center (Located near the White Flint Metro Station on the Red Line) Telephone Number: 301-822-9200 Fax Number: 301-822-9203

  3. Agenda31708 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    17, 2008 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings March 17, 2008 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting March 17, 2008 Where: Doubletree Hotel, 1515 Rhode Island Avenue, NW, Washington, D.C. (3 blocks off Dupont Circle Metro Stop) Telephone Number: 202-232-7000 Fax Number: 202-332-8436

  4. Agenda3807 | U.S. DOE Office of Science (SC)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    March 8-9, 2007 Nuclear Science Advisory Committee (NSAC) NSAC Home Meetings NSAC Members Charges/Reports Charter .pdf file (78KB) NP Committees of Visitors Federal Advisory Committees NP Home Meetings March 8-9, 2007 Print Text Size: A A A FeedbackShare Page DOE/NSF Nuclear Science Advisory Committee Meeting March 8-9, 2007 Where: Sheraton Crystal City Hotel, 1800 Jefferson Davis Highway, Arlington, VA (Metro Blue or Yellow Line to Crystal City Metro Stop) Telephone Number: 703-486-1111 Fax

  5. Sensitivity to methylmercury toxicity is enhanced in oxoguanine glycosylase 1 knockout murine embryonic fibroblasts and is dependent on cellular proliferation capacity

    SciTech Connect (OSTI)

    Ondovcik, Stephanie L.; Tamblyn, Laura; McPherson, John Peter; Wells, Peter G.

    2013-07-01

    Methylmercury (MeHg) is a persistent environmental contaminant with potent neurotoxic action for which the underlying molecular mechanisms remain to be conclusively delineated. Our objectives herein were twofold: first, to corroborate our previous findings of an increased sensitivity of spontaneously-immortalized oxoguanine glycosylase 1-null (Ogg1{sup ?/?}) murine embryonic fibroblasts (MEFs) to MeHg through generation of Simian virus 40 (SV40) large T antigen-immortalized wild-type and Ogg1{sup ?/?} MEFs; and second, to determine whether MeHg toxicity is proliferation-dependent. As with the spontaneously-immortalized cells used previously, the SV40 large T antigen-immortalized cells exhibited similar tendencies to undergo MeHg-initiated cell cycle arrest, with increased sensitivity in the Ogg1{sup ?/?} MEFs as measured by clonogenic survival and DNA damage. Compared to exponentially growing cells, those seeded at a higher density exhibited compromised proliferation, which proved protective against MeHg-mediated cell cycle arrest and induction of DNA double strand breaks (DSBs), measured by phosphorylation of the core histone H2A variant (H2AX) on serine 139 (?H2AX), and by its functional confirmation by micronucleus assessment. This enhanced sensitivity of Ogg1{sup ?/?} MEFs to MeHg toxicity using discrete SV40 immortalization corroborates our previous studies, and suggests a novel role for OGG1 in minimizing MeHg-initiated DNA lesions that trigger replication-associated DSBs. Furthermore, proliferative capacity may determine MeHg toxicity in vivo and in utero. Accordingly, variations in cellular proliferative capacity and interindividual variability in repair activity may modulate the risk of toxicological consequences following MeHg exposure. - Highlights: SV40 large T antigen-immortalized Ogg1{sup ?/?} cells are more sensitive to MeHg. Sensitivity to MeHg is dependent on cellular proliferation capacity. OGG1 maintains genomic integrity following MeHg-initiated DNA damage. OGG1 may limit MeHg-initiated DNA lesions that trigger replication-associated DSBs. Variations in proliferation and repair activity may modulate toxicological risk.

  6. Rigidity of a spherical capsule switches the localization of encapsulated particles between inner and peripheral regions under crowding condition: Simple model on cellular architecture

    SciTech Connect (OSTI)

    Shew, Chwen-Yang Kondo, Kenta; Yoshikawa, Kenichi

    2014-01-14

    We have investigated the inhomogeneous interior of confined spherical cavities as capsules containing encapsulated binary hard sphere mixtures for different compositions and cavity wall rigidity. Such a greatly simplified model manifests the effects of macromolecular crowding arising from excluded volume interactions in a tiny cell or a cellular nucleus. By fixing the number of large particles, the level of crowding is adjusted by changing the amount of small hard spheres in the cavity. For a rigid cavity, large spheres tend to pack in liquid-like order apart from the surface to the center of the cavity as the crowding level is increased. Whereas, for a soft cavity, larger spheres tend to blend with small spheres in the peripheral region at near the boundary of the cavity, and are susceptible to be depleted from the interior of the cavity as the cavity becomes more crowded. These results may help future elucidation of the thermodynamic pathways to stabilize the inhomogeneous structure of mixtures confined in cavities, such as the derepression of genome materials around the interior rim of the nucleus in a cancerous cell.

  7. 4-Methylumbelliferone inhibits hyaluronan synthesis by depletion of cellular UDP-glucuronic acid and downregulation of hyaluronan synthase 2 and 3

    SciTech Connect (OSTI)

    Kultti, Anne; Pasonen-Seppaenen, Sanna; Jauhiainen, Marjo; Rilla, Kirsi J.; Kaernae, Riikka; Pyoeriae, Emma; Tammi, Raija H.; Tammi, Markku I.

    2009-07-01

    Hyaluronan accumulation on cancer cells and their surrounding stroma predicts an unfavourable disease outcome, suggesting that hyaluronan enhances tumor growth and spreading. 4-Methylumbelliferone (4-MU) inhibits hyaluronan synthesis and retards cancer spreading in experimental animals through mechanisms not fully understood. These mechanisms were studied in A2058 melanoma cells, MCF-7 and MDA-MB-361 breast, SKOV-3 ovarian and UT-SCC118 squamous carcinoma cells by analysing hyaluronan synthesis, UDP-glucuronic acid (UDP-GlcUA) content, and hyaluronan synthase (HAS) mRNA levels. The maximal inhibition in hyaluronan synthesis ranged 22-80% in the cell lines tested. Active glucuronidation of 4-MU produced large quantities of 4-MU-glucuronide, depleting the cellular UDP-GlcUA pool. The maximal reduction varied between 38 and 95%. 4-MU also downregulated HAS mRNA levels: HAS3 was 84-60% lower in MDA-MB-361, A2058 and SKOV-3 cells. HAS2 was the major isoenzyme in MCF-7 cells and lowered by 81%, similar to 88% in A2058 cells. These data indicate that both HAS substrate and HAS2 and/or HAS3 mRNA are targeted by 4-MU. Despite different target point sensitivities, the reduction of hyaluronan caused by 4-MU was associated with a significant inhibition of cell migration, proliferation and invasion, supporting the importance of hyaluronan synthesis in cancer, and the therapeutic potential of hyaluronan synthesis inhibition.

  8. En/ant Plaza. S. W,. Washington. D.C. 20024.2174, Telephones...

    Office of Legacy Management (LM)

    ... Queen City Barrel Company Ohmart Brush Beryllium (Chester Street) Brush Beryllium (Perkins Avenue) Clecon bletals, Inc. (Horizons, Inc.) DuPont Grasselle Plant Harshaw Chemical ...

  9. SBOT NEW YORK BROOKHAVEN LAB POC Jill Clough-Johnston Telephone

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Related Equipment Merchant Wholesalers 423610 Electrical and Electronic Appliance, Television, and Radio Set Merchant Wholesalers 423620 Other Electronic Parts and Equipment...

  10. Solar cogeneration: Cimarron River station, Central Telephone and Utilities-Western Power

    SciTech Connect (OSTI)

    Harder, J.E.

    1981-04-01

    The site-specific conceptual design progress is described for a solar central receiver cogeneration facility at a Kansas utility. The process is described which led to the selection of the preferred solar cogeneration facility. The status of the conceptual design is presented. The evaluation of system performance is described. A test program is described that is to determine the magnitude of impact that local environmental factors have on collector system performance and to measure the direct normal insolation at the cogeneration facility site. The system specification is appended. (LEW)

  11. Telephone Flat Geothermal Development Project Environmental Impact Statement Environmental Impact Report. Final

    SciTech Connect (OSTI)

    1999-02-01

    This Final Environmental Impact Statement and Environmental Impact Report (Final EIS/EIR) has been prepared to meet the requirements of the National Environmental Policy Act (NEPA) and the California Environmental Quality Act (CEQA). The Proposed Action includes the construction, operation, and decommissioning of a 48 megawatt (gross) geothermal power plant with ancillary facilities (10-12 production well pads and 3-5 injection well pads, production and injection pipelines), access roads, and a 230-kilovolt (kV) transmission line in the Modoc National Forest in Siskiyou County, California. Alternative locations for the power plant site within a reasonable distance of the middle of the wellfield were determined to be technically feasible. Three power plant site alternatives are evaluated in the Final EIS/EIR.

  12. Application for use of BPA Right-of-Way

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ADDRESS: EMAIL ADDRESS: FAX NO: FAX NO: 7. LOCATION OF PROPERTY (Legal description of the property. This information is on your title, insurance policy, courthouse deed, or your...

  13. EIA-800

    U.S. Energy Information Administration (EIA) Indexed Site

    Email: Floor, Suite): Fax: (202) 586-1076 City: State: Zip: - Secure File Transfer: Electronic Transmission: City: State: Zip: - Contact Name: Phone No.: Ext: Fax No.: Questions? ...

  14. Effects of electromagnetic field stimulation on cellular signal transduction mechanisms: Analyses of the effects of low frequency electromagnetic fields on calcium spiking in ROS 17/2.8 cells. Final report

    SciTech Connect (OSTI)

    Sisken, B.F.; Sisken, J.E.

    1997-12-01

    The general goals of this work were to determine whether resting levels of cellular second messengers, especially calcium, are affected by low-level electromagnetic fields and the mechanisms that could lead to such changes. The work performed was directed at (1) verifying the report of McLeod et al (1990) that low frequency sinusoidal EMF can alter basal calcium fluctuations in cultured ROS 17/2.8 osteoblast-like cells and (2) reproducing the findings of Luben et al (1982) that pulsed electromagnetic fields can affect PTH-stimulated adenylate cyclase activity in osteoblasts. Initially a system was constructed so that cells could be exposed to sinusoidal electric fields using platinum electrodes. In this system, the electrodes were separated from the cells and culture medium by agar barriers. A series of experiments indicated that this system was subject to a significant, though little-known artifact in which a not well understood interaction between the electrodes and sodium ions in the medium or in plain salt solutions led to frequency and amplitude dependent emission of photons that are recorded by the detection system. They therefore designed and constructed an air gap reactor system that utilizes a ferromagnetic core to direct the magnetic flux generated by a sinusoidal coil. Studies on the effects of a 15 Hz pulsed electromagnetic field (PEMF) on cyclic AMP metabolism were performed on ROS 17/2.8 and MC3T3 cells.

  15. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  16. UNCLASSIFIED UNCLASSIFIED Nuclear Materials Management & Safeguards System

    National Nuclear Security Administration (NNSA)

    UNCLASSIFIED Nuclear Materials Management & Safeguards System CONTACT INFORMATION UPDATE REPORTING IDENTIFICATION SYMBOL (RIS) RIS: Address: Facility Name: CONTACTS Name Email: Phone/Fax Name Email: Phone/Fax Name Email: Phone/Fax Name Email: Phone/Fax Return Via Mail To: U.S Department Of Energy ATTN: NMMSS Staff NA-73, GTN 1000 Independence Avenue, SW Washington, DC 20585-1290 Return Via Fax To: 301-903-1998 Return Via E-Mail To: NMMSS@nnsa.doe.gov

  17. Cell Phone Detection Techniques

    SciTech Connect (OSTI)

    Pratt, Richard M.; Bunch, Kyle J.; Puzycki, David J.; Slaugh, Ryan W.; Good, Morris S.; McMakin, Douglas L.

    2007-10-01

    A team composed of Rick Pratt, Dave Puczyki, Kyle Bunch, Ryan Slaugh, Morris Good, and Doug McMakin teamed together to attempt to exploit cellular telephone features and detect if a person was carrying a cellular telephone into a Limited Area. The cell phones electromagnetic properties were measured, analyzed, and tested in over 10 different ways to determine if an exploitable signature exists. The method that appears to have the most potential for success without adding an external tag is to measure the RF spectrum, not in the cell phone band, but between 240 and 400MHz. Figures 1- 7 show the detected signal levels from cell phones from three different manufacturers.

  18. Beamline 12.3.1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    view it Life Sciences Division, Berkeley Lab Phone: (510) 486-8179 Fax: (510) 486-5298 Jane Tanamachi Life Sciences Division, Berkeley Lab Phone: (510) 495-22404 Fax: (510)...

  19. https://bluedart.phe.com/owa/?ae=Item&t=IPM.Note&id=RgAAAAA%2f3

    National Nuclear Security Administration (NNSA)

    Services 702-295-6582 702-858-5587 (cell) 702-295-7699 (fax) From: Michael Skougard ... Services 702-295-6582 702-858-5587 (cell) 702-295-7699 (fax) From: Morris, Patrick ...

  20. How to Submit a Freedom of Information Act Request

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and the Fax number will change to: (720)356-1780 Email: michele.altieri@go.doe.gov Please clearly mark the mailing envelope, fax or subject line of e-mail "Freedom of ...

  1. CAPITAL REGION

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    t 092007 15:28 FAX 301 903 4656 CAPITAL REGION 0 j002 SDOE F 1325.8 (8-89) EFG (0790) ... 092007 15:29 FAX 301 903 4656 CAPITAL REGION 003 * Implemented a more robust ...

  2. Microsoft Word - 13-NEAC-Plant-Closures

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... 263-1646 Fax: (608) 263-7451 www.engr.wisc.eduep Finding: There have been five ... 263-1646 Fax: (608) 263-7451 www.engr.wisc.eduep Recommendation: The NRT recommends ...

  3. eia-910.xls

    U.S. Energy Information Administration (EIA) Indexed Site

    Indicate unit of measure by placing an "X" in the appropriate box. Commercial Residential ... Address 1: OOG.SURVEYS@eia.gov Contact Name: Fax: (202) 586-1076 Ext: Fax No.: enter an "X...

  4. CONTRACT EMPLOYEES

    National Nuclear Security Administration (NNSA)

    Department Mailing PhoneFax Email or web site California California Department of ... Department Mailing PhoneFax Email or web site New York New York State Division of ...

  5. Victor Ramirez

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Victor Ramirez Title: Research Specialist 2 Office Tel. : (225) 578-9374 Fax: (225) 578-6954

  6. BPA-2012-00676-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    *** FO1.A OFFICE TILTS DATE: DUE DATE: Name: Francisco Carvalho Organization: University Professor Address: Phone: FAX: Email: Description of...

  7. Helical synthetic peptides that stimulate cellular cholesterol efflux

    DOE Patents [OSTI]

    Bielicki, John K.; Natarajan, Pradeep

    2010-04-06

    The present invention provides peptides comprising at least one amphipathic alpha helix and having an cholesterol mediating activity and a ABCA stabilization activity. The invention further provides methods of using such peptides.

  8. Tools and Models for Integrating Multiple Cellular Networks

    SciTech Connect (OSTI)

    Gerstein, Mark

    2015-11-06

    In this grant, we have systematically investigated the integrated networks, which are responsible for the coordination of activity between metabolic pathways in prokaryotes. We have developed several computational tools to analyze the topology of the integrated networks consisting of metabolic, regulatory, and physical interaction networks. The tools are all open-source, and they are available to download from Github, and can be incorporated in the Knowledgebase. Here, we summarize our work as follow. Understanding the topology of the integrated networks is the first step toward understanding its dynamics and evolution. For Aim 1 of this grant, we have developed a novel algorithm to determine and measure the hierarchical structure of transcriptional regulatory networks [1]. The hierarchy captures the direction of information flow in the network. The algorithm is generally applicable to regulatory networks in prokaryotes, yeast and higher organisms. Integrated datasets are extremely beneficial in understanding the biology of a system in a compact manner due to the conflation of multiple layers of information. Therefore for Aim 2 of this grant, we have developed several tools and carried out analysis for integrating system-wide genomic information. To make use of the structural data, we have developed DynaSIN for protein-protein interactions networks with various dynamical interfaces [2]. We then examined the association between network topology with phenotypic effects such as gene essentiality. In particular, we have organized E. coli and S. cerevisiae transcriptional regulatory networks into hierarchies. We then correlated gene phenotypic effects by tinkering with different layers to elucidate which layers were more tolerant to perturbations [3]. In the context of evolution, we also developed a workflow to guide the comparison between different types of biological networks across various species using the concept of rewiring [4], and Furthermore, we have developed CRIT for correlation analysis in systems biology [5]. For Aim 3, we have further investigated the scaling relationship that the number of Transcription Factors (TFs) in a genome is proportional to the square of the total number of genes. We have extended the analysis from transcription factors to various classes of functional categories, and from individual categories to joint distribution [6]. By introducing a new analytical framework, we have generalized the original toolbox model to take into account of metabolic network with arbitrary network topology [7].

  9. Science Against Stress: Research Shows Way to Some Cellular Relief

    Broader source: Energy.gov [DOE]

    Researchers at Brookhaven National Lab (BNL) are studying a tumor-suppressor protein called p53 to further the Office of Science’s long-standing mission to understand how radioactive materials affect the human genome.

  10. Defining the Molecular-Cellular-Field Continuum of Mercury Detoxification

    SciTech Connect (OSTI)

    Miller, Susan M.

    2014-09-04

    Hg is of special interest to DOE due to past use at the Oak Ridge Reservation (ORR). Its facile redox [Hg2+/0] chemistry, bonding to carbon [e.g. MeHg+] and unique physical properties [e.g., Hg0 volatility] underlie a complex global Hg cycle involving biotic and abiotic chemical and physical transport and transformations in soils, sediments, waterways and the atmosphere. Facultative and anaerobic bacteria make MeHg+, which is neurotoxic to wildlife and humans. Sustainable stewardship requires eliminating both MeHg+ and even more toxic Hg2+, which is also the substrate for methylation. The proteins encoded by the mer locus in aerobic and facultative mercury resistant (HgR) bacteria convert soil or waterborne Hg2+ or MeHg+ to less toxic, gaseous Hg0. HgR microbes live in highly Hg-contaminated sites and depress MeHg+ formation >500-fold in such zones. So, enhancing the capacity of natural HgR microbes to remove Hg2+/MeHg+ from wetlands and waterways is a logical component of contaminated site stewardship. To apply enhancement in the field requires knowing how the HgR pathway works including the metabolic demands it makes on the cell, i.e., the entire cell is the relevant catalytic unit. HgR loci occur in metabolically diverse bacteria and unique mer-host co-evolution has been found. In this project we extended our previous studies of mer enzymes in ?-proteobacteria, which are abundant in high Hg areas of the ORR to include studies of mer enzymes from HgR ?-proteobacteria and HgR actinobacteria, which also increase in the high Hg regions of the ORR. Specifically, we (1) examined interactions between structural compoenents of MerA and MerB enzymes from ?-proteobacteria, (2) investigated effects of mutations on kinetic efficiency of Hg2+ reduction by ?-proteobacterial MerA, (3) cloned and performed initital characterization of MerA and MerB enzymes from Streptomyces lividans, an actinobacterium, (4) cloned and performed initial characterization of a fused MerB-MerA protein from Ochrobactrum anthropi, an ?-proteobacterium, (5) investigate the extent of Hg isotope fractionation that occurs with purified ?-proteobacterial MerA.

  11. On the Interaction between Marine Boundary Layer Cellular Cloudiness...

    Office of Scientific and Technical Information (OSTI)

    The investigation focuses on the non-precipitating closed-cell state and the precipitating open-cell state at low geostrophic wind speed. The Advanced Research WRF model is used to ...

  12. Whole blood analysis rotor assembly having removable cellular sedimentation bowl

    DOE Patents [OSTI]

    Burtis, C.A.; Johnson, W.F.

    1975-08-26

    A rotor assembly for performing photometric analyses using whole blood samples is described. Following static loading of a gross blood sample within a centrally located, removable, cell sedimentation bowl, the red blood cells in the gross sample are centrifugally separated from the plasma, the plasm displaced from the sedimentation bowl, and measured subvolumes of plasma distributed to respective sample analysis cuvettes positioned in an annular array about the rotor periphery. Means for adding reagents to the respective cuvettes are also described. (auth)

  13. Nickel decreases cellular iron level and converts cytosolic aconitase...

    Office of Scientific and Technical Information (OSTI)

    the mechanism for the observed effects, human embryonic kidney (HEK) cells over ... B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International ...

  14. Cellular morphometry of the bronchi of human and dog lungs

    SciTech Connect (OSTI)

    Robbins, E.S.

    1992-09-01

    Quantitative data of the human bronchial epithelial cells at possible risk for malignant transformation in lung cancer is crucial for accurate radon dosimetry and risk analysis. The locations and other parameters of the nuclei which may be damaged by [alpha] particles must be determined and compared in different airway generations, among smokers, non-smokers and ex-smokers, between men and women and in people of different ages. This proposal includes extended morphometric studies on electron micrographs of human epithelium of defined airway generations and in parallel on electron micrographs of the dog bronchial lining. The second part of this proposal describes studies to quantitate the cycling bronchial epithelial population(s) using proliferation markers and immunocytochemistry on frozen and paraffin sections and similar labeling of isolated bronchial epithelial cells sorted flow cytometry.

  15. Sustainable Nano-Materials: What is happening at the cellular...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    organic medium Coating of nanoparticles surface with a surfactant High specific area : high amount of surfactant Chemical modification of nanoparticles surface O O O ( ) n ( ) m ...

  16. Structure of adenovirus bound to cellular receptor car

    DOE Patents [OSTI]

    Freimuth, Paul I.

    2004-05-18

    Disclosed is a mutant adenovirus which has a genome comprising one or more mutations in sequences which encode the fiber protein knob domain wherein the mutation causes the encoded viral particle to have significantly weakened binding affinity for CARD1 relative to wild-type adenovirus. Such mutations may be in sequences which encode either the AB loop, or the HI loop of the fiber protein knob domain. Specific residues and mutations are described. Also disclosed is a method for generating a mutant adenovirus which is characterized by a receptor binding affinity or specificity which differs substantially from wild type. In the method, residues of the adenovirus fiber protein knob domain which are predicted to alter D1 binding when mutated, are identified from the crystal structure coordinates of the AD12knob:CAR-D1 complex. A mutation which alters one or more of the identified residues is introduced into the genome of the adenovirus to generate a mutant adenovirus. Whether or not the mutant produced exhibits altered adenovirus-CAR binding properties is then determined.

  17. Structure of adenovirus bound to cellular receptor car

    DOE Patents [OSTI]

    Freimuth, Paul I.

    2007-01-02

    Disclosed is a mutant CAR-DI-binding adenovirus which has a genome comprising one or more mutations in sequences which encode the fiber protein knob domain wherein the mutation causes the encoded viral particle to have a significantly weakened binding affinity for CAR-DI relative to wild-type adenovirus. Such mutations may be in sequences which encode either the AB loop, or the HI loop of the fiber protein knob domain. Specific residues and mutations are described. Also disclosed is a method for generating a mutant adenovirus which is characterized by a receptor binding affinity or specificity which differs substantially from wild type.

  18. On the reversibility of transitions between closed and open cellular...

    Office of Scientific and Technical Information (OSTI)

    We show that the asymmetry in the two-way transition occurs even for very rapid drop concentration replenishment. The primary barrier to recovery is the loss in turbulence kinetic ...

  19. Fluorescent dyes for probing cellular functions associated with...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    To fully understand the processes involved in biomineralization in magnetotactic bacteria, it is necessary to obtain the time-resolved data of biomineralization of magnetite...

  20. Microsoft Word - Revision 2 06-16-2014.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 Nevada National Security Site Profile Sheet Nevada National Security Site Profile Sheet - Revision 2 - June 2014 A. Waste Generator or Classified Matter Originator Information 1. Company name: 2. Address: 3. Generator/Originator Facility: 4. Primary Technical Contact: Email: Phone: Fax: 5. DOE Point of Contact: Email: Phone: Fax: 6. Certification Official: Email: Phone: Fax: 7. Generator's EPA Identification Number (If profile involves hazardous waste): 8. If the waste or classified matter is

  1. SAVANNAH RIVER REGIONAL DIVERSIFICATION INITIATIVE

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Address: City: State: Zip Code: Phone: Fax: County: Federal Identification Number: Tax Status: Description of item or items to be purchased: (use an additional sheet if...

  2. EIA-Voluntary Reporting of Greenhouse Gases Program - Contact

    U.S. Energy Information Administration (EIA) Indexed Site

    Contact Voluntary Reporting of Greenhouse Gases Program Contact For more information on the Voluntary Reporting of Greenhouse Gases Program, contact us via e-mail, phone, fax, or ...

  3. Carbon Emissions: Iron and Steel Industry

    U.S. Energy Information Administration (EIA) Indexed Site

    Contact: Stephanie Battles Stephanie.Battles@eia.doe.gov (Phone: (202) 586-7237) FAX: 202-586-0018 Contact Us URL: http:www.eia.govemeuefficiencycarbonemissionssteel...

  4. Carbon Emissions: Petroleum Refining Industry

    U.S. Energy Information Administration (EIA) Indexed Site

    Contact: Stephanie Battles Stephanie.Battles@eia.doe.gov (Phone: (202) 586-7237) FAX: 202-586-0018 Contact Us URL: http:www.eia.govemeuefficiencycarbonemissionspetroleum...

  5. Carbon Emissions: Stone, Clay, and Glass Industry

    U.S. Energy Information Administration (EIA) Indexed Site

    Contact: Stephanie Battles Stephanie.Battles@eia.doe.gov (Phone: (202) 586-7237) FAX: 202-586-0018 Contact Us URL: http:www.eia.govemeuefficiencycarbonemissionsstone...

  6. Triangle Universities Nuclear Laboratory : 2011

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Important TUNL Phone Numbers Location Phone (Area Code 919) TUNL MAIN OFFICE (BOX 90308) 660-2600/660-2626 CONTROL ROOM 660-2602 TUNL LAB LEVEL 660-2603 / 660-2604 TUNL ELECTRONICS SHOP 660-2543 TUNL PAGING 660-2641 PHYSICS PAGING 660-2585 TUNL FAX MACHINE 660-2634 DUKE PHYSICS DEPARTMENT 660-2500 /2501/ 2502/2491/2492 DUKE PHYSICS DEPT. / FAX 660-2525 UNC PHYSICS DEPT. / FAX 962-2078 / 962-0480 NCSU PHYSICS DEPT. / FAX 515-2521 / 515-6538 FEL CONTROL ROOM 660-2646 HIGS TARGET ROOM 660-2673 KECK

  7. Carbon Emissions: Chemicals Industry

    U.S. Energy Information Administration (EIA) Indexed Site

    Stephanie Battles Stephanie.Battles@eia.doe.gov (Phone: (202) 586-7237) FAX: 202-586-0018 URL: http:www.eia.govemeuefficiencycarbonemissionschemicals.html Contact Us File...

  8. 2005 Residential Energy Consumption Survey

    U.S. Energy Information Administration (EIA) Indexed Site

    Street Address Contractor's City, State, and ZIP Code Or you may FAX the completed ... and to the Office of Information and Regulatory Affairs, Office of Management and ...

  9. Message----- From: WEBMASTER, ODE Sent: Monday, October

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    information-- Name: Mike Nartker Organization: Weapons Complex Monitor Address: 4301 Connecticut Ave. NW Suite 132 Washington, D.C., 20008 Fax number: 202-296-2805 Phone number:...

  10. DOE Headquarters Contact Information: Employee Concerns Program...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Employee Concerns Program http:energy.govdiversity 1 DOE Headquarters Contact Information: Employee Concerns Program Patricia Zarate Phone: 202-586-2248 Fax: 202-586-3075 ...

  11. FormCampusMachineShopsLabs.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Highway Baton Rouge, Louisiana 70806 225-578-8887 225-578-6954 (Fax) Form for Campus Machine Shops and Labs Purchaser's Name Phone ...

  12. BPA-2012-01387-FOIA Request`

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FOIA request: *** Name: JOHN A QUALLS Organization: (TFOY) PORT ANGELES MAINTENANCE CREW Address: Phone: No FAX number provided Email: Page 1 of 1...

  13. Meeting with Greg Horton

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CAES Facility and Business Equipment Repair Information * Maintenance or repair of business equipment o For repair of copiers, fax machines, printer, etc., contact one of the CAES...

  14. BPA-2012-01627-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    No FAX number provided Email: Description of Request: emails sent to attorney Kassandra Brown between october 2011-december 2011 asking for phone records in 2008. Preferred format:...

  15. Department

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Address: country Phone Number: Fax Number: E-mail: 20111110100931 Evan J. Kaufman Robbins Geller Rudman &38; Dowd LLP 58 South Service Road, Suite 200 Melville, NY 11747...

  16. BPA-2011-00973-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    To: FOIA Subject: FOIA Request The following is a New FOIA request: Name: Mrytle June Chapman Organization: Estate of Daphne Ellen Holtgrieve Address:. Phone: No FAX number...

  17. BPA-2011-00999-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    following is a New FOIA request: ***:*** Name: Myrtle June Chapman Organization: Estate of Daphne Ellen Holtgrieve Address: Phone: No FAX number...

  18. BPA-2011-00430-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    following is a New FOIA request: *** Name: Myrtle June Chapman Organization: Estate of Daphne Holtgrieve Address: Phone: No FAX number provided...

  19. HAN F OR

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fax Number: E-mail: United States na na Reasonably Describe Records: Description: Industrial Hygiene Chemical Vapor Technical Basis RPP-22491 Revision 1 Optional...

  20. NEAC-RT ComLtr 11.1.12

    Broader source: Energy.gov (indexed) [DOE]

    Engineering Physics 1500 Engineering Drive Madison WI 53706 Phone: (608) 263-1646 Fax: (608) 263-7451 www.engr.wisc.eduep Nuclear Engineering Engineering Physics Engineering ...

  1. SUPPORT ADMINISTRATIVE SERVICES FOR THE NATIONAL ENERGY TECHNOLOGY

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... convenience copiers. 5. Daily log of faxes sent and ... staff to resolve scheduling conflicts. 169 C. ... contract. 5. The award fee pool is detailed in Section B of ...

  2. BPA-2015-00051-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    on this form. Name Carrie Henderson Email Organiz ation Mailing Address City State Phone FAX Ex. xxx-xxx-xxxx Date Redd: 1082014 Due Date: 1162014 Tracking Number;...

  3. Connecticut Department of Energy and Environmental Protection...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Secretary for Energy Chief of Staff Attachment 1 14728 Pipeline Avenue Suite E Chino Hills California 91709 Phone: 626-583-1116 Fax: 626-577-8439 ...

  4. Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    coming to SSRL) before beam time. Spokesperson: Institution: Email: Degree: Work Phone: Fax: Principal Investigator: Email: Work Phone: Collaborators: Institution: (if...

  5. BPA-2011-01633-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FOIA request: DATE: 9 ***x*** DUE DATE: 9a- Name: Richard Dijk van y Organization: Another Way BPA t OG Address: Phone: 3 -JD- 01 No FAX number...

  6. BPA-2011-01733-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The following is a New FOIA request: *** Name: Richard van Dijk Organization: Another Way BPA Address: Phone: No FAX number provided Email: Description...

  7. BPA-2011-01630-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The following is a New FOIA request: *** Name: Richard van Dijk Organization: Another Way BPA Address: Phone:: No FAX number provided Email:...

  8. BPA-2012-00236-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    To: FOIA Subject: FOLA Request The following is a New FOIA request: Name: Richard van Dijk Organization: Another Way BPA Address: Phone: No FAX number provided Email: Description...

  9. BPA-2011-01634-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    is a New FOIA request: ****** DUE DATE: Name: Richard van Dijk Organization: Another Way BPA LOG Address: - W( + O6 Phone: No FAX number provided...

  10. BPA-2011-01732-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The following is a New FOIA request: *** *** Name: Richard van Dijk Organization: Another Way BPA Address: Phone: No FAX number provided Email: RECEIVED BY...

  11. BPA-2011-01780-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The following is a New FOIA request: *** *** Name: Richard van Dijk Organization: Another Way BPA Address: Phone: No FAX number provided Email: Description...

  12. BPA-2011-01635-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The following is a New FOIA request: *** Name: Richard van Dijk Organization: Another Way BPA Address: Phone: No FAX number provided Email: RfXEtvED BY...

  13. BPA-2011-02052-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The following is a New FOIA request: *** Name: Richard van Dijk Organization: AnotherwayBPA Address: Phone: No FAX number provided Email: Description...

  14. https://www.fossil.energy.gov/app/fergas/ApplicationAction.go

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Code: Phone Number: ext. Fax: E-mail: Report Contact (Monthly Reports) Information: First Name: Last Name: Middle Initial: PositionTitle: Country: Street: City: StateProvince:...

  15. Henry Bellamy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Henry Bellamy, Ph.D. Title: Professor - Research PX Beamline Manager Office Tel. : (225) 578-9342 Fax: (225) 578-6954

  16. BPA-2011-00748-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Name: Mark L Bubenik Organization: Northwest Justice Project Address: 715 Tacoma Ave. So. Tacoma, WA 98402 Phone: (253) 272 7879 ext.262 FAX: (253) 272 8226 Email:...

  17. BPA-2011-00834-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    7202145856 FAX: 3039886409 Email: bmcbee@bentekenergy.com Description of Request: Hello, I am seeking data associated with hourly load, thermal generation, wind generation...

  18. BPA-2011-00698-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    7202145856 FAX: 3039886409 Email: bmcbee@bentekenergy.com Description of Request: Hello, I am seeking data associated with hourly imports and exports of electricity to and...

  19. Map to CAMD

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Advanced Microstructures & Devices Louisiana State University 6980 Jefferson Hwy. Baton Rouge, LA 70806 225-578-8887 Main Office Tel. 225-578-6954 Fax

  20. AcqGuideChapter39pt2.doc

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Wide Web sites; multimedia; and office equipment (such as copiers and fax machines). ... 39.2 (June 2005) 4 * Video and multimedia products; * Self contained, closed ...

  1. EM SSAB Local Board Contacts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EM SSAB Local Board Contacts EM SSAB Local Board Contacts EM SSAB Federal Coordinators Hanford Kristen Skopeck Phone: (509) 376-5803 Fax: (509) 376-1563 E-mail: kristen.skopeck@rl.doe.gov Oak Ridge Melyssa Noe Phone: (865) 241-3315 Fax: (865) 241-6932 E-mail: noemp@oro.doe.gov Idaho Robert Pence Phone: (208) 526-6518 Fax: (208) 526-8789 E-mail: pencerl@id.doe.gov Paducah Robert Smith Phone: (270) 441-6821 Fax: (270) 441-6801 E-mail: robert.smith@lex.doe.gov Nevada Kelly Snyder Phone: (702)

  2. United States Government

    Broader source: Energy.gov (indexed) [DOE]

    ... Nuclear weapon design, fabrication, and utilization. X Radiological warfare. X ... X 101603 THU 15:46 FAX 423 241 3897 OIG - H-*- HQ 1007 AUDIT DATABASE INFORMATION SHEET ...

  3. Contact Us | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contact Us Contact Us Division Director Supratik Guha Phone: 630-252-7740 Deputy Division Director Tijana Rajh Phone: 630.252.5334 Fax: 630.252.6866 Assistant Division Director Deborah O'Rourke Phone: 630.252.3810 Fax: 630.252.6866 Associate Division Director/ CNM User Programs & Operations Katie Carrado Gregar Phone: 630-252-7968 Fax: 630-252-6866 Executive Secretary Laurie Eichberger Phone: 630.252.7570 Fax: 630.252.6866 Manager, Building & Systems Operations Ron Tollner Phone:

  4. Microsoft Word - 1897.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Identification Device User print name Government AgencySponsored Company Visitor's Z Number or ID Number Email Address Phone Number Fax Number Mail Stop and Office Location...

  5. The directory of United States coal & technology export resources. Profiles of domestic US corporations, associations and public entities, nationwide, which offer products or services suitable for export, relating to coal and its utilization

    SciTech Connect (OSTI)

    Not Available

    1994-01-01

    The purpose of this directory is to provide a listing of available U.S. coal and coal related resources to potential purchasers of those resources abroad. The directory lists business entities within the US which offer coal related resources, products and services for sale on the international market. Each listing is intended to describe the particular business niche or range of product and/or services offered by a particular company. The listing provides addresses, telephones, and telex/fax for key staff in each company committed to the facilitation of international trade. The content of each listing has been formulated especially for this directory and reflects data current as of the date of this edition. The directory listings are divided into four primary classifications: coal resources; technology resources; support services; and financing and resource packaging. The first three of which are subdivided as follows: Coal Resources -- coal derivatives, coal exporters, and coal mining; Technology Resources -- advanced utilization, architects and engineers, boiler equipment, emissions control and waste disposal systems, facility construction, mining equipment, power generation systems, technical publications, and transport equipment; Support Services -- coal transport, facility operations, freight forwarders, sampling services and equipment, and technical consultants. Listings for the directory were solicited on the basis of this industry breakdown. Each of the four sections of this directory begins with a matrix illustrating which companies fall within the particular subclassifications specific to that main classification. A general alphabetical index of companies and an index by product/service classification are provided following the last section of the directory.

  6. Science Education Staff | Princeton Plasma Physics Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Staff Andrew Zwicker Head of Science Education Telephone: 609-243-2150 Email: azwicker@pppl.gov Read Andrew's blog Arturo Dominguez Senior Program Leader Telephone: 609-243-2197 Email: adomingu@pppl.gov Deedee Ortiz Program Administrator Telephone: 609-243-2785 Email: dortiz@pppl.gov Shannon Greco Program Leader Telephone: 609-243-2208 Email: sgreco@pppl.gov Larry Guttadora Technical Specialist Telephone: 609-243-3144 Email: lguttadora@pppl.gov Sophia Gershman Senior Program Advisor Telephone:

  7. U.S. Department of Commerce National Technical Information Service

    Office of Legacy Management (LM)

    ... City, Utah 84123-2547 Phone (801) 266-7700 Web Page: www.datachem.com FAX (801) 268-9992 ... Utah 84123-2547 Phone (801) 266-7700 , B-5 Web Page: www.datachem.com FAX (801) 268-9992 ...

  8. Directory Listings | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    employees including their telephone number, routing symbol, and location (buildingroom number) A listing of Headquarters staff by organization including their telephone...

  9. BPA-2011-01001-FOIA Correspondence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    electronic record, telephone messages, voicemails or other sound records, notes of personal conferences, telephone conversations or personal meetings. This also includes...

  10. BPA-2011-01001-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    record, telephone messages, voice-mails or other sound recordings, notes of personal conferences, telephone conversations or personal meetings. It also includes...

  11. Compression of 1-mm-thick M9763 cellular silicone foam under...

    Office of Scientific and Technical Information (OSTI)

    OSTI Identifier: 1183540 Report Number(s): LLNL-TR--667442 DOE Contract Number: ... Full Text preview image File size NAView Full Text View Full Text DOI: 10.21721183540

  12. Sustainable Nano-Materials: What is happening at the cellular level?

    Broader source: Energy.gov [DOE]

    Presentation for the Sustainable Nanomaterials Workshop by Georgia Institute of Technology held on June 26, 2012

  13. Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration

    SciTech Connect (OSTI)

    Simarro, Maria; Gimenez-Cassina, Alfredo; Kedersha, Nancy; Lazaro, Jean-Bernard; Adelmant, Guillaume O.; Marto, Jarrod A.; Rhee, Kirsten; Tisdale, Sarah; Danial, Nika; Benarafa, Charaf; Orduna, Anonio; Anderson, Paul

    2010-10-22

    Research highlights: {yields} Five members of the FAST kinase domain-containing proteins are localized to mitochondria in mammalian cells. {yields} The FASTKD3 interactome includes proteins involved in various aspects of mitochondrial metabolism. {yields} Targeted knockdown of FASTKD3 significantly reduces basal and maximal mitochondrial oxygen consumption. -- Abstract: Fas-activated serine/threonine phosphoprotein (FAST) is the founding member of the FAST kinase domain-containing protein (FASTKD) family that includes FASTKD1-5. FAST is a sensor of mitochondrial stress that modulates protein translation to promote the survival of cells exposed to adverse conditions. Mutations in FASTKD2 have been linked to a mitochondrial encephalomyopathy that is associated with reduced cytochrome c oxidase activity, an essential component of the mitochondrial electron transport chain. We have confirmed the mitochondrial localization of FASTKD2 and shown that all FASTKD family members are found in mitochondria. Although human and mouse FASTKD1-5 genes are expressed ubiquitously, some of them are most abundantly expressed in mitochondria-enriched tissues. We have found that RNA interference-mediated knockdown of FASTKD3 severely blunts basal and stress-induced mitochondrial oxygen consumption without disrupting the assembly of respiratory chain complexes. Tandem affinity purification reveals that FASTKD3 interacts with components of mitochondrial respiratory and translation machineries. Our results introduce FASTKD3 as an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration.

  14. A New Slant on a Cellular Balancing Act - The Copper-sensing...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    an essential component of many metalloenzymes, but free intracellular copper is highly toxic. Because of this copper within cells is very tightly controlled, with specific copper...

  15. Crossing Over: Nanostructures that Move Electrons and Ions Across Cellular Membranes

    SciTech Connect (OSTI)

    Ajo-Franklin, C. M.; Noy, A.

    2015-04-27

    Critical biological processes such as energy generation and signal transduction are driven by the flow of electrons and ions across the membranes of living cells. As a result, there is substantial interest in creating nanostructured materials that control transport of these charged species across biomembranes. The recent advances in the synthesis of de novo and protein nanostructures for transmembrane ion and electron transport and the mechanistic understanding underlying this transport are described. Moreover, this body of work highlights the promise such nanostructures hold for directing transmembrane transport of charged species as well as challenges that must be overcome to realize that potential.

  16. Mechanisms underlying cellular responses of cells from haemopoietic tissue to low

    SciTech Connect (OSTI)

    Kadhim, Munira A

    2012-08-22

    The above studies will provide fundamental mechanistic information relating genetic predisposition to important low dose phenomena, and will aid in the development of Department of Energy policy, as well as radiation risk policy for the public and the workplace. We believe the proposed studies accurately reflect the goals of the DOE low dose program. To accurately define the risks associated with human exposure to relevant environmental doses of low LET ionizing radiation, it is necessary to completely understand the biological effects at very low doses (i.e. less than 0.1 Gy), including the lowest possible dose, that of a single electron track traversal. At such low doses, a range of studies have shown responses in biological systems which are not related to the direct interaction of radiation tracks with DNA. The role of these "œnon-targeted" responses in critical tissues is poorly understood and little is known regarding the underlying mechanisms. Although critical for dosimetry and risk assessment, the role of individual genetic susceptibility in radiation risk is not satisfactorily defined at present. The aim of the proposed grant is to critically evaluate non-targeted effects of ionizing radiation with a focus on the induction of genomic instability (GI) in key stem cell populations from haemopoietic tissue. Using stem cells from two mouse strains (CBA/CaH and C57BL/6J) known to differ in their susceptibility to radiation effects, we plan to carefully dissect the role of genetic predisposition in these models on genomic instability. We will specifically focus on the effects of low doses of low LET radiation, down to the dose of 10mGy (0.01Gy) X-rays. Using conventional X-ray and we will be able to assess the role of genetic variation under various conditions at a range of doses down to the very low dose of 0.01Gy. Irradiations will be carried out using facilities in routine operation for such studies. Mechanistic studies of instability in different cell lineages will include the role of cytokines which have been shown to be in the initiation of instability. These studies also aim to uncover the possible mechanism of the initiation, perpetuation and delayed pathways of the instability response using relevant biological endpoints i.e. chromosomal instability, apoptosis induction, cytokine and gene array analysis. Integral to these studies will be an assessment of the role of genetic susceptibility in these responses, using CBA/CaH and C57BL/6J mice. The overall results suggest that low dose low LET X-irradiation induced delayed GI in both CBA/CaH and C57BL/6J haemopoeitic tissue. Using several biological approaches, some key strain and dose-specific differences have been identified in radiation-induced signalling in the initiation and perpetuation of the instability process. Furthermore, the induction of non-targeted radiation effects and genetic dependency may be linked to the use of alternative signalling pathways and mechanisms which have potential implications on evaluation of non-targeted effects in radiation risk assessment.

  17. Open-air direct current plasma jet: Scaling up, uniformity, and cellular control

    SciTech Connect (OSTI)

    Wu, S.; Wang, Z.; Huang, Q.; Lu, X.; Ostrikov, K.

    2012-10-15

    Atmospheric-pressure plasma jets are commonly used in many fields from medicine to nanotechnology, yet the issue of scaling the discharges up to larger areas without compromising the plasma uniformity remains a major challenge. In this paper, we demonstrate a homogenous cold air plasma glow with a large cross-section generated by a direct current power supply. There is no risk of glow-to-arc transitions, and the plasma glow appears uniform regardless of the gap between the nozzle and the surface being processed. Detailed studies show that both the position of the quartz tube and the gas flow rate can be used to control the plasma properties. Further investigation indicates that the residual charges trapped on the inner surface of the quartz tube may be responsible for the generation of the air plasma plume with a large cross-section. The spatially resolved optical emission spectroscopy reveals that the air plasma plume is uniform as it propagates out of the nozzle. The remarkable improvement of the plasma uniformity is used to improve the bio-compatibility of a glass coverslip over a reasonably large area. This improvement is demonstrated by a much more uniform and effective attachment and proliferation of human embryonic kidney 293 (HEK 293) cells on the plasma-treated surface.

  18. Synthesis of heterocycles: Indolo (2,1-a) isoquinolines, renewables, and aptamer ligands for cellular imaging

    SciTech Connect (OSTI)

    Beasley, Jonathan

    2013-05-07

    In this thesis, we explore both total syntheses and methodologies of several aromatic heterocyclic molecules. Extensions of the Kraus indole synthesis toward 2-substituted and 2,3-disubstituted indoles, as well as biologically attractive indolo[2,1-a]isoquinolines are described. Recent renewable efforts directed to commodity maleic acid and the first reported furan-based ionic liquids are described. Our total synthesis of mRNA aptamer ligand PDC-Gly, and its dye coupled forms, plus aminoglycoside dye coupled ligands used in molecular imaging, are described.

  19. International symposium on cellular and molecular biology of phosphate and phosphorylated compounds in microorganisms: Proceedings

    SciTech Connect (OSTI)

    1993-12-31

    This report contains the abstracts of papers presented at the conference. Attention is focused on the following topics: regulation of phosphate metabolism in bacteria; structure-function of alkaline phosphatase; regulation of phosphate metabolism in yeast; transport of phosphate and phosphorylated compounds; and phosphate regulation in pathogenesis and secondary metabolism.

  20. Effects of ozone on the respiratory health, allergic sensitization, and cellular immune system in children

    SciTech Connect (OSTI)

    Zwick, H.; Popp, W.; Wagner, C.; Reiser, K.; Schmoeger, J.B.; Boeck, A.H.; Herkner, K.; Radunsky, K. )

    1991-11-01

    To investigate the lasting effects of high ozone concentrations under environmental conditions, we examined the respiratory health, pulmonary function, bronchial hyperresponsiveness to methacholine, allergic sensitization, and lymphocyte subpopulations of 10- to 14-yr-old children. A total of 218 children recruited from an area with high ozone concentrations (Group A) were tested against 281 children coming from an area with low ozone concentrations (Group B). As to subjective complaints, categorized as 'usually cough with or without phlegm,' 'breathlessness,' and 'susceptibility to chest colds,' there was no difference between the two groups. The lung function parameters were similar, but in Group A subjects' bronchial hyperresponsiveness occurred more frequently and was found to be more severe than in Group B (29.4 versus 19.9%, p less than 0.02; PD20 2,100 {plus minus} 87 versus 2,350 {plus minus} 58 micrograms, p less than 0.05). In both groups the number of children who had been suffering from allergic diseases and sensitization to aeroallergens, found by means of the skin test, was the same. Comparison of the total IgE levels showed no difference at all between the two groups. As far as the white blood cells are concerned, the total and differential cell count was the same, whereas lymphocyte subpopulations showed readily recognizable changes.

  1. Tissue architecture and function: dynamic reciprocity via extra- and intra-cellular matrices

    SciTech Connect (OSTI)

    Xu, Ren; Boudreau, Aaron; Bissell, Mina J

    2008-12-23

    Mammary gland development, functional differentiation, and homeostasis are orchestrated and sustained by a balance of biochemical and biophysical cues from the organ's microenvironment. The three-dimensional microenvironment of the mammary gland, predominantly 'encoded' by a collaboration between the extracellular matrix (ECM), hormones, and growth factors, sends signals from ECM receptors through the cytoskeletal intracellular matrix to nuclear and chromatin structures resulting in gene expression; the ECM in turn is regulated and remodeled by signals from the nucleus. In this chapter, we discuss how coordinated ECM deposition and remodeling is necessary for mammary gland development, how the ECM provides structural and biochemical cues necessary for tissue-specific function, and the role of the cytoskeleton in mediating the extra - to intracellular dialogue occurring between the nucleus and the microenvironment. When operating normally, the cytoskeletal-mediated dynamic and reciprocal integration of tissue architecture and function directs mammary gland development, tissue polarity, and ultimately, tissue-specific gene expression. Cancer occurs when these dynamic interactions go awry for an extended time.

  2. Synchrotron-based X-ray computed tomography during compression loading of cellular materials

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cordes, Nikolaus L.; Henderson, Kevin; Stannard, Tyler; Williams, Jason J.; Xiao, Xianghui; Robinson, Mathew W. C.; Schaedler, Tobias A.; Chawla, Nikhilesh; Patterson, Brian M.

    2015-04-29

    Three-dimensional X-ray computed tomography (CT) of in situ dynamic processes provides internal snapshot images as a function of time. Tomograms are mathematically reconstructed from a series of radiographs taken in rapid succession as the specimen is rotated in small angular increments. In addition to spatial resolution, temporal resolution is important. Thus temporal resolution indicates how close together in time two distinct tomograms can be acquired. Tomograms taken in rapid succession allow detailed analyses of internal processes that cannot be obtained by other means. This article describes the state-of-the-art for such measurements acquired using synchrotron radiation as the X-ray source.

  3. Toward Real-time Modeling of Human Heart Ventricles at Cellular...

    Office of Scientific and Technical Information (OSTI)

    CA Sponsoring Org: USDOE Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL SCIENCES; 97 MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE

  4. Apparatus and method for measuring single cell and sub-cellular photosynthetic efficiency

    DOE Patents [OSTI]

    Davis, Ryan Wesley; Singh, Seema; Wu, Huawen

    2013-07-09

    Devices for measuring single cell changes in photosynthetic efficiency in algal aquaculture are disclosed that include a combination of modulated LED trans-illumination of different intensities with synchronized through objective laser illumination and confocal detection. Synchronization and intensity modulation of a dual illumination scheme were provided using a custom microcontroller for a laser beam block and constant current LED driver. Therefore, single whole cell photosynthetic efficiency, and subcellular (diffraction limited) photosynthetic efficiency measurement modes are permitted. Wide field rapid light scanning actinic illumination is provided for both by an intensity modulated 470 nm LED. For the whole cell photosynthetic efficiency measurement, the same LED provides saturating pulses for generating photosynthetic induction curves. For the subcellular photosynthetic efficiency measurement, a switched through objective 488 nm laser provides saturating pulses for generating photosynthetic induction curves. A second near IR LED is employed to generate dark adapted states in the system under study.

  5. Compression of 1-mm-thick M9763 cellular silicone foam under...

    Office of Scientific and Technical Information (OSTI)

    Abstract not provided. Authors: Small, W. 1 + Show Author Affiliations Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States) Publication Date: 2014-11-13 OSTI ...

  6. Processing and characterization of multi-cellular monolithic bioceramics for bone regenerative scaffolds

    SciTech Connect (OSTI)

    Ari-Wahjoedi, Bambang; Ginta, Turnad Lenggo; Parman, Setyamartana; Abustaman, Mohd Zikri Ahmad

    2014-10-24

    Multicellular monolithic ceramic body is a ceramic material which has many gas or liquid passages partitioned by thin walls throughout the bulk material. There are many currently known advanced industrial applications of multicellular ceramics structures i.e. as supports for various catalysts, electrode support structure for solid oxide fuel cells, refractories, electric/electronic materials, aerospace vehicle re-entry heat shields and biomaterials for dental as well as orthopaedic implants by naming only a few. Multicellular ceramic bodies are usually made of ceramic phases such as mullite, cordierite, aluminum titanate or pure oxides such as silica, zirconia and alumina. What make alumina ceramics is excellent for the above functions are the intrinsic properties of alumina which are hard, wear resistant, excellent dielectric properties, resists strong acid and alkali attacks at elevated temperatures, good thermal conductivities, high strength and stiffness as well as biocompatible. In this work the processing technology leading to truly multicellular monolithic alumina ceramic bodies and their characterization are reported. Ceramic slip with 66 wt.% solid loading was found to be optimum as impregnant to the polyurethane foam template. Mullitic ceramic composite of alumina-sodium alumino disilicate-Leucite-like phases with bulk and true densities of 0.852 and 1.241 g cm{sup ?3} respectively, pore linear density of 35 cm{sup ?1}, linear and bulk volume shrinkages of 7-16% and 32 vol.% were obtained. The compressive strength and elastic modulus of the bioceramics are ?0.5-1.0 and ?20 MPa respectively.

  7. Crossing Over: Nanostructures that Move Electrons and Ions Across Cellular Membranes

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ajo-Franklin, C. M.; Noy, A.

    2015-04-27

    Critical biological processes such as energy generation and signal transduction are driven by the flow of electrons and ions across the membranes of living cells. As a result, there is substantial interest in creating nanostructured materials that control transport of these charged species across biomembranes. The recent advances in the synthesis of de novo and protein nanostructures for transmembrane ion and electron transport and the mechanistic understanding underlying this transport are described. Moreover, this body of work highlights the promise such nanostructures hold for directing transmembrane transport of charged species as well as challenges that must be overcome to realizemore » that potential.« less

  8. Mechanisms of disease: epithelial-mesenchymal transition and back again: does cellular plasticity fuel neoplastic progression?

    SciTech Connect (OSTI)

    Bissell, Mina J; Turley, Eva A.; Veiseh, Mandana; Radisky, Derek C.; Bissell, Mina J.

    2008-02-13

    Epithelial-mesenchymal transition (EMT) is a conversion that facilitates organ morphogenesis and tissue remodeling in physiological processes such as embryonic development and wound healing. A similar phenotypic conversion is also detected in fibrotic diseases and neoplasia, which is associated with disease progression. EMT in cancer epithelial cells often seems to be an incomplete and bi-directional process. In this Review, we discuss the phenomenon of EMT as it pertains to tumor development, focusing on exceptions to the commonly held rule that EMT promotes invasion and metastasis. We also highlight the role of the RAS-controlled signaling mediators, ERK1, ERK2 and PI3-kinase, as microenvironmental responsive regulators of EMT.

  9. Synchrotron-based X-ray computed tomography during compression loading of cellular materials

    SciTech Connect (OSTI)

    Cordes, Nikolaus L.; Henderson, Kevin; Stannard, Tyler; Williams, Jason J.; Xiao, Xianghui; Robinson, Mathew W. C.; Schaedler, Tobias A.; Chawla, Nikhilesh; Patterson, Brian M.

    2015-04-29

    Three-dimensional X-ray computed tomography (CT) of in situ dynamic processes provides internal snapshot images as a function of time. Tomograms are mathematically reconstructed from a series of radiographs taken in rapid succession as the specimen is rotated in small angular increments. In addition to spatial resolution, temporal resolution is important. Thus temporal resolution indicates how close together in time two distinct tomograms can be acquired. Tomograms taken in rapid succession allow detailed analyses of internal processes that cannot be obtained by other means. This article describes the state-of-the-art for such measurements acquired using synchrotron radiation as the X-ray source.

  10. Eugene Stewart | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Technician Sr. Eugene Stewart Telephone 630.252.4466 E-mail estewart

  11. Peter Fuesz | Argonne National Laboratory

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    Scientific Associate Peter Fuesz Telephone 630.252.7151 E-mail fuesz

  12. Edward J. Daniels | Argonne National Laboratory

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    Edward J. Daniels Sr. Energy Systems Scientist Telephone (630) 252-5279 E-mail edaniels

  13. Ron Tollner | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ron Tollner Manager, Building & Systems Operations Telephone 630.252.4457 E-mail tollner

  14. Sharon Nykolyshyn | Argonne National Laboratory

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    Sharon Nykolyshyn Executive Secretary - EGS Telephone 630-252-3739 E-mail snykolyshyn

  15. David Eckstein | Argonne National Laboratory

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    Eckstein Principal Application Developer Telephone 630.252.7918 E-mail deckstein@anl.gov

  16. Alex Martinson | Argonne National Laboratory

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    Alex Martinson Telephone (630) 252-7520 E-mail martinson

  17. Microsoft PowerPoint - Wayne_Shirley_Decoupling_Mechanics_and_Issues.ppt [Compatibility Mode]

    Energy Savers [EERE]

    Decoupling: Mechanics and Issues Presentation to the New Mexico Public Regulation Commission Energy Efficiency Incentives Workshop July 16-17, 2008 The Regulatory Assistance Project Presented by Wayne Shirley The Regulatory Assistance Project 110 B Water St. Hallowell, Maine USA 04347 Tel: 207.623.8393 50 State Street, Suite 3 Montpelier, Vermont USA 05602 Tel: 802.223.8199 27 Penny Lane Cedar Crest, New Mexico USA 87008 Tel: 505.286.4486 Fax: 207.623.8369 Fax: 802.223.8172 E-Fax: 773.347.1512

  18. Microsoft PowerPoint - Wayne_Shirley_Overview_of_Incentives2008_08_22.ppt [Compatibility Mode]

    Energy Savers [EERE]

    Utility Incentives Presentation to the Kansas Corporation Commission E Effi i I i W k h Energy Efficiency Incentives Workshop August 26, 2008 Presented by The Regulatory Assistance Project Presented by Wayne Shirley The Regulatory Assistance Project 110 B Water St. Hallowell, Maine USA 04347 Tel: 207.623.8393 50 State Street, Suite 3 Montpelier, Vermont USA 05602 Tel: 802.223.8199 27 Penny Lane Cedar Crest, New Mexico USA 87008 Tel: 505.286.4486 Fax: 207.623.8369 Fax: 802.223.8172 E-Fax:

  19. Community Reuse Organization | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Community Reuse Organization Community Reuse Organization HANFORD Carl Adrian TRIDEC 7130 West Grandridge Blvd., Suite A Kennewick, WA 99336 (509) 735-1000, ext. 225 (509) 735-6609 (FAX) cadrian@tridec.org LOS ALAMOS Kathy Keith, Executive Director Regional Development Corporation 706 Bond Street Espanola, NM 87532 (505) 820-1226 (505) 983-8654 (FAX) info@rdcnm.org MOUND Mike Grauwelman, President MMCIC P.O. Box 232 Miamisburg, OH 45343-0232 (937) 865-4462 (937) 865-4431 (FAX) mikeg@mound.com

  20. Tribal Points of Contacts | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Points of Contacts Tribal Points of Contacts US DOE-Office of Environmental Management 1000 Independence Avenue, SW Washington, DC 20585 ph: (202) 586-5944 fax: (202) 586-5000 Richland Operations Office- Hanford Indian Nations Program P.O. Box 550- MSIN A7-75 Richland, WA 99352 ph: (509) 376-6332 fax: (509) 376-1563 West Valley Demonstration Project Tribal Government Liaison P.O. Box 191- 10282 Rock Springs Road West Valley, NY 14171 ph: (716) 942-4629 fax: (716) 942-2068 Albuquerque Operations

  1. Microsoft Word - 11319 Final.doc

    Office of Environmental Management (EM)

    Decoupling: Mechanics and Issues Presentation to the New Mexico Public Regulation Commission Energy Efficiency Incentives Workshop July 16-17, 2008 The Regulatory Assistance Project Presented by Wayne Shirley The Regulatory Assistance Project 110 B Water St. Hallowell, Maine USA 04347 Tel: 207.623.8393 50 State Street, Suite 3 Montpelier, Vermont USA 05602 Tel: 802.223.8199 27 Penny Lane Cedar Crest, New Mexico USA 87008 Tel: 505.286.4486 Fax: 207.623.8369 Fax: 802.223.8172 E-Fax: 773.347.1512

  2. Submitting Organization Sandia National Laboratories

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hongyou Fan Advanced Materials Laboratory 1001 University Boulevard SE Albuquerque, NM 87106 Country: USA Phone: 505-272-7128 Fax: 505-272-7336 hfan@sandia.gov Contact Person Glenn D. Kubiak, Director Sandia National Laboratories PO Box 969 Mail Stop 9405 Livermore, CA 94551-0969 USA Phone: 925-294-3375 Fax: 925- 294-3403 kubiak@sandia.gov Joint Entry Lockheed Martin Aeronautics Mail Zone 2893, PO Box 748 City: Fort Worth, Texas 76101 USA Earl Stromberg Phone: (817) 763-7376 Fax: (817) 762-6911

  3. Smart repeater system for communications interoperability during multi-agency law enforcement operations

    SciTech Connect (OSTI)

    Crutcher, R.I.; Jones, R.W.; Moore, M.R.; Smith, S.F.; Tolley, A.L.; Rochelle, R.W.

    1996-12-31

    A prototype smart repeater that provides interoperability capabilities for radio communication systems in multi-agency and multi-user scenarios is being developed by the Oak Ridge National Laboratory. The smart repeater functions as a deployable communications platform that can be dynamically reconfigured to cross-link the radios of participating federal, state, and local government agencies. This interconnection capability improves the coordination and execution of multi-agency operations, including coordinated law enforcement activities and general emergency or disaster response scenarios. The repeater provides multiple channels of operation in the 30--50, 118--136, 138--174, and 403--512 MHz land mobile communications and aircraft bands while providing the ability to cross-connect among multiple frequencies, bands, modulation types, and encryption formats. Additionally, two telephone interconnects provide links to the fixed and cellular telephone networks. The 800- and 900-MHz bands are not supported by the prototype, but the modular design of the system accommodates future retrofits to extend frequency capabilities with minimal impact to the system. Configuration of the repeater is through a portable personal computer with a Windows-based graphical interface control screen that provides dynamic reconfiguration of network interconnections and formats.

  4. Rechargeable lithium battery for use in applications requiring a low to high power output

    DOE Patents [OSTI]

    Bates, John B.

    1997-01-01

    Rechargeable lithium batteries which employ characteristics of thin-film batteries can be used to satisfy power requirements within a relatively broad range. Thin-film battery cells utilizing a film of anode material, a film of cathode material and an electrolyte of an amorphous lithium phosphorus oxynitride can be connected in series or parallel relationship for the purpose of withdrawing electrical power simultaneously from the cells. In addition, such battery cells which employ a lithium intercalation compound as its cathode material can be connected in a manner suitable for supplying power for the operation of an electric vehicle. Still further, by incorporating within the battery cell a relatively thick cathode of a lithium intercalation compound, a relatively thick anode of lithium and an electrolyte film of lithium phosphorus oxynitride, the battery cell is rendered capable of supplying power for any of a number of consumer products, such as a laptop computer or a cellular telephone.

  5. Rechargeable lithium battery for use in applications requiring a low to high power output

    DOE Patents [OSTI]

    Bates, John B.

    1996-01-01

    Rechargeable lithium batteries which employ characteristics of thin-film batteries can be used to satisfy power requirements within a relatively broad range. Thin-film battery cells utilizing a film of anode material, a film of cathode material and an electrolyte of an amorphorus lithium phosphorus oxynitride can be connected in series or parallel relationship for the purpose of withdrawing electrical power simultaneously from the cells. In addition, such battery cells which employ a lithium intercalation compound as its cathode material can be connected in a manner suitable for supplying power for the operation of an electric vehicle. Still further, by incorporating within the battery cell a relatively thick cathode of a lithium intercalation compound, a relatively thick anode of lithium and an electrolyte film of lithium phosphorus oxynitride, the battery cell is rendered capable of supplying power for any of a number of consumer products, such as a laptop computer or a cellular telephone.

  6. Oak Ridge Site Specific Advisory Board Contacts | Department...

    Office of Environmental Management (EM)

    Connie Jones, EPA Liaison U.S. EPA Region 4 Restoration and Site Evaluation Branch Superfund Division 61 Forsyth Street, SW Atlanta, GA 30303-3415 Phone: (404) 562-8551 Fax: (404) ...

  7. Public Involvement Plan Public Involvement Plan

    Energy Savers [EERE]

    ... U.S. Environmental Protection Agency FFB 61 Forsyth Street S.W. Atlanta, GA 30303-3104 EPA Hotline: 1-800-241-1745 EPA Public Affairs Fax: (404) 562-8174 Web site: www.epa.gov...

  8. ASHRAE

    Energy Savers [EERE]

    ASHRAE Technology for a Better Environment 1791 Tullie Circle, NE Atlanta, GA 30329-2305 USA Tel 404.636.8400 Fax 404.321.5478 http:www.ashrae.org Lynn G. ...

  9. --No Title--

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    575 Sand Hill Road * Mail Stop 103 * Menlo Park, CA 94025-7015 650-926-2288 * Fax 650-926-4695 SLAC is operated by Stanford University for the U.S. Department of Energy SLAC...

  10. Microsoft Word - Inspection of TRUPACT-III Changes.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hazardous Waste Bureau 2905 Rodeo Park Drive East, Building 1 Santa Fe, New Mexico 87505-6303 Phone (505) 476-6000 Fax (505) 476-6030 www.nmenv.state.nm.us DAVE MARTIN...

  11. BPA-2014-01924-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Harkonen, P.Eng. Consulting Specialist, Power Systems NORDMIN ENGINEERING LTD. 160 Logan Avenue Thunder Bay, ON, CANADA P7A 6R1 Phone: (807) 683-4858 Fax: (807) 344-0404 ww W...

  12. T.G. Hinton: Radioactive Contaminants in Aquatic Ecosystems ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Aquatic Ecosystems Thomas G. Hinton Savannah River Ecology Laboratory P O Drawer E, Aiken, SC 29802 (803) 725-7454 office (803) 725-3309 fax thinton(at)uga.edu Dr. Hinton's...

  13. T.G. Hinton: Human and Ecological Risks from Radioactive Contaminants...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Human and Ecological Risks from Radioactive Contaminants Thomas G. Hinton Savannah River Ecology Laboratory P O Drawer E, Aiken, SC 29802 (803) 725-7454 office (803) 725-3309 fax...

  14. https://sweis.nv.doe.gov/References/Szymanski%202010.txt

    National Nuclear Security Administration (NNSA)

    TIMOTHY R. SZYMANSKI PEIO PUBLIC EDUCATIONINFORMATION OFFICER LAS VEGAS FIRE & RESCUE 500 N. Casino Center Blvd. Las Vegas, NV 89101 Cell: 702-303-2993 Office: 702-229-0145 Fax: ...

  15. Contact Us - U.S. Energy Information Administration (EIA) - U...

    Gasoline and Diesel Fuel Update (EIA)

    Energy Consumption, End Uses and Efficiency Contacts Fax: (202)-586-0018 Alternative Fuels Cynthia Amezcua 202-586-1658 cynthia.amezcua@eia.gov Commercial Buildings Joelle Michaels ...

  16. Oak Ridge Site Specific Advisory Board * P.O...

    Office of Environmental Management (EM)

    TN 37831 Phone: 865-241-4583, 865-241-4584, 1-800-382-6938 * Fax: 865-574-3521 * Internet: www.oakridge.doe.govemssab Many Voices Working for the Community Oak Ridge Site...

  17. Office of Inspector General | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    FAX: (202) 586-4902 Rickey R. Hass Acting Inspector General More about Rickey Hass Stay Connected Facebook logo Twitter logo Subscribe RSS logo Subscribe to receive the latest...

  18. BPA-2014-01713-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kiley Scott Email Kiley.Scott@SNCLavalin.com Organizatio SNC Lavalin T&D n Mailing 150 Whitten Rd Address City Augusta State ME Phone 315-842-0999 Ex. xxx-xxx-xxxx FAX Ex....

  19. Microsoft Word - Ref_Spectra_1MB.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reference X-Ray Spectra of Metal Foils Exafs Materials 871 El Cerro Blvd., Danville, CA 94526 USA phone: (925) 838-7162 fax: (925) 838-4630 email: exafsmaterials@sbcglobal.net...

  20. BPA-2013-00058-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Michaelyn .ia..,i Lc -s No organization provided Address: 185 South Fairview Lane Sonora, Ca 95370 Phone: 2095996000 No FAX number provided Email: michaelyn@tri-technic.com...

  1. SSRLUO 2002 Executive Committee Members | Stanford Synchrotron...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Pingitore University of Texas at El Paso Environmental & Geosciences El Paso, TX 79968-0555 Phone: 915-747-5754 Fax: 915-747-5073 E-mail: nick@geo.utep.edu MACROMOLECULAR...

  2. Buildings and Energy in the 1980's -- Index Page

    U.S. Energy Information Administration (EIA) Indexed Site

    Manager Phone: (202) 586-7237 Fax: (202) 586-0018 URL: http:www.eia.govemeurecs1f.html If you are having any technical problems with this site, please contact the EIA...

  3. Changes in Energy Intensity 1985-1991

    U.S. Energy Information Administration (EIA) Indexed Site

    586-7237 Fax: (202) 586-0018 URL: http:www.eia.govemeumecsmecs91intensitymecs1b.html File Last Modified: May 25, 1996 If you are having any technical problems with this...

  4. BPA-2015-01591-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    o n this form. Name Email Organization Mailing Address City State Phone FAX William Campbell I 'b' '6' J J Zip Reasonably Describe Records Describe the specific record(s) you...

  5. STEAB Annual Report 2010-2011

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... and promoting a culture of bottom-up change and ... of energy efficiency techniques and renewable energy ... Fax: 541-858-4790 Cell: 541-944-8992 E-mail: ...

  6. Directory of Points of Contact for the DOE Technical Standard...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Phone: 865-576-5031 Fax: 865-241-3497 Email: beckj@orau.gov ORO Standards Manager Jenni Hamilton U.S. Department of Energy Oak Ridge Operations Office P.O. Box 2001, MS-AD440 Oak ...

  7. Alternative Fuels Data Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    including loan terms, see the AlabamaSAVES website. Point of Contact Liz Cochran Program Manager Alabama Department of Economic and Community Affairs Phone: (334) 353-4380 Fax:...

  8. BPA-2010-01892-FOIA Request

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    To: FOIA Subject: FOIA Request The following is a New FOIA request: Name: Joseph M. Williams No organization provided Address: . Phone: Ex 6 No FAX number provided Email: Ex 6...

  9. seca-core-tech-sofc-seal-meeting | netl.doe.gov

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    NETL Conference Services Information Conference Services National Energy Technology Laboratory Phone: (412) 386-6044 FAX: (412) 386-6486 E-mail: kimberly.yavorsky@netl.doe.gov...

  10. afe | netl.doe.gov

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    Presentation NETL Conference Services Information National Energy Technology Laboratory Phone: (412) 386-6044 FAX: (412) 386-6486 E-mail: kimberly.yavorsky@netl.doe.gov Disclaimer...

  11. seca-model-simul-team-integration-mtg | netl.doe.gov

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    NETL Conference Services Information Conference Services National Energy Technology Laboratory Phone: (412) 386-6044 FAX: (412) 386-6486 E-mail: kimberly.yavorsky@netl.doe.gov...

  12. sco2 workshop | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Technology Laboratory Phone: (412) 386-6044 FAX: (412) 386-6486 E-mail: kimberly.yavorsky@netl.doe.gov Disclaimer This report was prepared as an account of work...

  13. Contact Us | Linac Coherent Light Source

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contact Us Tel: 650-926-2246 | Fax: 650-926-3615 LCLSSLAC is located on 426 acres of Stanford University property, just three miles west of the main campus. The main entrance to...

  14. Contact Us | Stanford Synchrotron Radiation Lightsource

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Contact Us Stanford Synchrotron Radiation Lightsource SLAC National Accelerator Laboratory 2575 Sand Hill Road, MS 69 Menlo Park, CA 94025 Tel: 650-926-4000 Fax: 650-926-4100 SSRL...

  15. Contact Us | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    NNSA Production Office Contact Us Contact Us NPO Public Affairs Manager Steven Wyatt (865) 576-9918 NPO Mailing Address NNSA Production Office PO Box 2050 Oak Ridge, TN 37831 Fax...

  16. Min Chen | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Min Chen Min Chen Min Chen Min Chen Research Affiliate E-mail: min.chen@sydney.edu.au Phone: +61 2 9036 5006 Fax: +61 2 9351 4119 Website: University of Sydney Research Affiliate...

  17. Michelle Liberton | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Michelle Liberton Michelle Liberton Michelle Liberton Michelle Liberton Research Scientist E-mail: mllibert@wustl.edu Phone: 314.935.8133 Fax: 314.935.4432 Website: Washington...

  18. Gabriel Montao | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Gabriel Montao Gabriel Montao Gabriel Montao Gabriel Montao Principal Investigator E-mail: gbmon@lanl.gov Phone: 505.284.8236 Fax: 505.284.7778 Website: Los Alamos National...

  19. Elizabeth Dorland | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dorland Elizabeth Dorland Elizabeth Dorland Elizabeth Dorland Communications Director E-mail: dorland@wustl.edu Phone: 314.935.6094 Fax: 314.935.4925 Office: Brauer Hall 2023...

  20. Microsoft Word - 2011 EIA-23S Form.doc

    U.S. Energy Information Administration (EIA) Indexed Site

    of Energy, EIA Ben Franklin Station P O Box 279 Washington DC 20044-0279 OR Fax to ... Company Name: Street or P. O. Box: City, State, Zip Code: Contact Person (Please Print): ...

  1. U.S. DEPARTMENT OF ENERGY ENERGY INFORMATION ADMINISTRATION

    U.S. Energy Information Administration (EIA) Indexed Site

    of Energy, EIA Ben Franklin Station P O Box 279 Washington DC 20044-0279 OR Fax to ... Contact Information Company Name: Street or P. O. Box: City, State, Zip Code: Contact ...

  2. Slide14 | OSTI, US Dept of Energy, Office of Scientific and Technical

    Office of Scientific and Technical Information (OSTI)

    Information Questions? Walt Warnick, Ph.D. Director, Office of Scientific and Technical Information U.S. Department of Energy Office of Science (301) 903-7996 (301) 903-8972 (fax) walter.warnick@science.doe.gov

  3. Morris. Alexander From: Sent: To: Subject: FOIA-Central@hq.doe...

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  4. With the onset of fall and the return of school, the Bonneville...

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  5. Lisa Gerhardt

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    Lisa Gerhardt Lisa Gerhardt Lisa Gerhardt Big Data Architect lgerhardt@lbl.gov Phone: (510) 486-4680 Fax: (510) 486-4316 1 Cyclotron Road Mail Stop 943-256 Berkeley, CA 94720 ...

  6. Microsoft Word - Privacy_Contact_Listing_September_21_2010

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  7. Technical Standards Managers

    Broader source: Energy.gov (indexed) [DOE]

    Phone: (202) 586-7963 Fax: (202) 586-3859 stephen.lucke @nnsa.doe.gov DOE-NFO Sheila M. Harris TSM U.S. Department of Energy Centerra Nevada DOENNSANevada Field O ce MS 576...

  8. AcqGuide47pt1.doc

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... at: U.S. Department of Energy Office of Headquarters Procurement Services Attn: Peggy L. Fuller Code ME-641 Washington, D.C. 20585 Tele: (202) 287-1464 Fax No. (202) 287-1453 If an ...

  9. Erin Plut | Photosynthetic Antenna Research Center

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Erin Plut Erin Plut Erin Plut Administrative Coordinator E-mail: eplut@wustl.edu Phone: 314.935.4499 Fax: 314.935.4925 Office: Seigle Hall 430 Website: PARC Steering Committee Operations Committee

  10. SUSANA MARTINEZ Governor JOHN A SANCHEZ Lieutenant Governor

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    28, 2011 NEW MEXICO ENVIRONMENT DEPARTMENT Water and Waste Management Division Harold Runnels Building 1190 St. Francis Drive, Santa Fe, NM 87505 Phone (505) 827-1758 Fax (505)...

  11. Contact Us | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

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  12. Fourth RFI_Comment on Regulatory Review.pdf

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    To that end, the Executive Order requires, among other things, that: * Agencies propose or ... 206-544-5000, extension 1; fax 206-766-5680; Internet https: www.myboeingfleet.com. ...

  13. United_CoolAir_Ex Parte Meeting Memo.pdf

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Rod Beever Vice President United CoolAir Corp. 491 E. Princess St. York, PA 17403 Tel (717) 843-4311 (x 113) Fax (717) 854-4462 Email: rbeever@unitedcoolair.com ...

  14. Federal Buildings Supplemental Survey 1993

    U.S. Energy Information Administration (EIA) Indexed Site

    cubic feet or 1,000 cubic feet; fuel oil--fuel-tank data; and district heating and cooling--the entire district or system. Respondents could Fax completed worksheets that...

  15. Susan Martindale

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    ... FAX: (317) 472-0106 rjohnson@utilitylaw.us J. D. Jojola Citizen Information Resource ... 586-3670 Gary.Lanthrum@rw.doe.gov Doug Larson Western Interstate Energy Board 1515 ...

  16. Iodine solubility in a low-activity waste borosilicate glass...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4651; fax: +1 (509)372 5997. E-mail address: brian.riley@pnnl.gov (B.J. Riley). Journal of Nuclear Materials 452 (2014) 178-188 Contents lists available at ScienceDirect...

  17. Iodine valence and local environments in borosilicate waste glasses...

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    319 5226; fax: +1 (202) 319 4469. E-mail address: davidm@vsl.cua.edu (D.A. McKeown). Journal of Nuclear Materials 456 (2015) 182-191 Contents lists available at ScienceDirect...

  18. TUE

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    142006 TUE 10:21 FAX 5093762020 AdvanceMed Hanford 141 007 OMS App."121D().CC42 , 11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS 0 The above numbered 60lici18tion...

  19. SUSANA MARTINEZ Governor JOHN A. SANCHEZ Lieutenant Governor

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    5, 2011 NEW MEXICO ENVIRONMENT DEPARTMENT Hazardous Waste Bureau 2905 Rodeo Park Drive East, Building 1 Santa Fe, New Mexico 87505-6303 Phone (505) 476-6000 Fax (505) 476-6030...

  20. SHIPROCK, NEW MEXICO

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    ... 2486601 Fax: (970) 248-7636 False ID 620 62 1 94 1 427 842 True ID 619 619 940 425 841 Sanple Type Duplicate Equiplnent Blank Duplicate Equipnent Blank Duplicate Associated ...