National Library of Energy BETA

Sample records for rat neth er

  1. DJS CLAIM ER

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    t c 5 "NEUTR DJS CLAIM ER This report was prepared a s an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, make any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference

  2. OoEr

    Office of Legacy Management (LM)

    OoEr 1325.8 (MW . fR)W-001 United States Government memoranduln I~J-U Department of Energy II&& yz;; EH-421 (W. A. Williams, 427-1719) SUBJECT: Elimination of the Sites froa the Formerly Utilized Sltes Remedial Actiorr Prograa Tb: The File In 1990, with the assistance of Hr. reviewed a nulaber of sites that had Doug Tonkay and Hs. Michelle LaWs, I services to the Fernald facility as fomerly provided goods and/or subcontractors. sites, recommendations were made to For 24 of.these _

  3. Protein folding in the ER.

    SciTech Connect (OSTI)

    Stevens, F. J.; Argon, Y.; Biosciences Division; Univ. of Chicago

    1999-10-01

    The endoplasmic reticulum (ER) is a major protein folding compartment for secreted, plasma membrane and organelle proteins. Each of these newly-synthesized polypeptides folds in a deterministic process, affected by the unique conditions that exist in the ER. An understanding of protein folding in the ER is a fundamental biomolecular challenge at two levels. The first level addresses how the amino acid sequence programs that polypeptide to efficiently arrive at a particular fold out of a multitude of alternatives, and how different sequences obtain similar folds. At the second level are the issues introduced by folding not in the cytosol, but in the ER, including the risk of aggregation in a molecularly crowded environment, accommodation of post-translational modifications and the compatibility with subsequent intracellular trafficking. This review discusses both the physicochemical and cell biological constraints of folding, which are the challenges that the ER molecular chaperones help overcome.

  4. Ternary Dy-Er-Al magnetic refrigerants

    DOE Patents [OSTI]

    Gschneidner, Jr., Karl A.; Takeya, Hiroyuki

    1995-07-25

    A ternary magnetic refrigerant material comprising (Dy.sub.1-x Er.sub.x)Al.sub.2 for a magnetic refrigerator using the Joule-Brayton thermodynamic cycle spanning a temperature range from about 60K to about 10K, which can be adjusted by changing the Dy to Er ratio of the refrigerant.

  5. Ternary Dy-Er-Al magnetic refrigerants

    DOE Patents [OSTI]

    Gschneidner, K.A. Jr.; Takeya, Hiroyuki

    1995-07-25

    A ternary magnetic refrigerant material comprising (Dy{sub 1{minus}x}Er{sub x})Al{sub 2} for a magnetic refrigerator using the Joule-Brayton thermodynamic cycle spanning a temperature range from about 60K to about 10K, which can be adjusted by changing the Dy to Er ratio of the refrigerant. 29 figs.

  6. Environmental release summary (ERS) database CY 1997

    SciTech Connect (OSTI)

    Gleckler, B.P.

    1998-07-01

    This report discusses the Environmental Release Summary (ERS) database. The current needs of the Effluent and Environmental database is continually modified to fulfill monitoring (EEM) program (managed by Waste Management Federal Services of Hanford, Incorporated, Air and Water Services Organization). Changes are made to accurately calculate current releases, to affect how past releases are calculated. This document serves as a snap-shot of the database and software for the CY-1997 data and releases. This document contains all of the relevant data for calculating radioactive-airborne and liquid effluent. The ERS database is the official repository for the CY-1997 ERS release reports and the settings used to generate those reports. As part of the Tri-Party Agreement, FDH is committed to provide a hard copy of the ERS database for Washington State Department of Ecology, upon request. This document also serves as that hard copy for the last complete calendar year.

  7. ER85773 | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ER85773 Downhole Oxyfuel Steam/CO2 Generator for Production of Gas from Hydrates ER85773 Last Reviewed 11/1/2011 Project Goal The goal of this project is to demonstrate a novel, oxyfuel downhole steam generator (DSG) that will efficiently recover gas from methane hydrate deposits while simultaneously reducing emissions and also having the potential for carbon dioxide (CO2) sequestration. Success will lead to commercialization of a downhole combustor for natural gas production. Performer

  8. ErSol Thin Film GmbH formerly ErSol New Technologies GmbH ENT...

    Open Energy Info (EERE)

    ErSol Thin Film GmbH formerly ErSol New Technologies GmbH ENT Jump to: navigation, search Name: ErSol Thin Film GmbH (formerly ErSol New Technologies GmbH (ENT)) Place: Germany...

  9. ARM CLASIC ER2 CRS/EDOP

    SciTech Connect (OSTI)

    Gerald Heymsfield

    2010-12-20

    Data was taken with the NASA ER-2 aircraft with the Cloud Radar System and other instruments in conjunction with the DOE ARM CLASIC field campaign. The flights were near the SGP site in north Central Oklahoma and targeted small developing convection. The CRS is a 94 GHz nadir pointing Doppler radar. Also on board the ER-2 was the Cloud Physics Lidar (CPL). Seven science flights were conducted but the weather conditions did not cooperate in that there was neither developing convection, or there was heavy rain.

  10. Low temperature properties of some Er-rich intermetallic compounds

    SciTech Connect (OSTI)

    K.A. Gshneidner,jr; A.O. Pecharsky; L.Hale; V.K. Pecharsky

    2004-09-30

    The low temperature volumetric heat capacity ({approx}3.5 to 350 K) and magnetic susceptibility ({approx}4 to 320 K) of Er{sub 3}Rh, Er{sub 3}Ir, Er{sub 3}Pt, Er{sub 2}Al, and Er{sub 2}Sn have been measured. All of the compounds order antiferromagnetically (or ferrimagnetically), and most exhibit more than one magnetic ordering transition. The volumetric heat capacities in general are smaller than those of the prototype magnetic regenerator materials, except for Er{sub 3}Ir in the 12 to 14 K temperature range.

  11. DOE-ER-STD-6001-92

    Broader source: Energy.gov [DOE]

    Implementation Guide for Quality Assurance Programs for Basic and Applied Research Canceled (January, 2003) This Implementation Guide is intended to assist management at DOE-ER sponsored facilities in the process of developing and implementing Quality Assurance Programs (QAPs) that satisfy the requirements of DOE Order 5700.6C.

  12. Audit Report: ER-FS-99-03

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Report Number: ER-FS-99-03 Eastern Regional Audit Office Date of Issue: May 1999 Oak ... IG Report No. ER-FS-99-03 CUSTOMER RESPONSE FORM The Office of Inspector General has a ...

  13. Audit Report: ER-FS-99-04

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DATE: May 27, 1999 REPLY TO: IG-36 (A98PR015) REPORT NUMBER: ER-FS-99-04 SUBJECT: Results ... Audit Liaison Division IG Report No. ER-FS-99-04 CUSTOMER RESPONSE FORM The Office ...

  14. ER-12-1 completion report

    SciTech Connect (OSTI)

    Russell, C.E.; Gillespie, D.; Cole, J.C.; Drellack, S.L.

    1996-12-01

    The objective of drillhole ER-12-1 was to determine the hydrogeology of paleozoic carbonate rocks and of the Eleana Formation, a regional aquitard, in an area potentially downgradient from underground nuclear testing conducted in nearby Rainier Mesa. This objective was addressed through the drilling of well ER-12-1 at N886,640.26 E640,538.85 Nevada Central Coordinates. Drilling of the 1094 m (3588 ft) well began on July 19, 1991 and was completed on October 17, 1991. Drilling problems included hole deviation and hole instability that prevented the timely completion of this borehole. Drilling methods used include rotary tri-cone and rotary hammer drilling with conventional and reverse circulation using air/water, air/foam (Davis mix), and bentonite mud. Geologic cuttings and geophysical logs were obtained from the well. The rocks penetrated by the ER-12-1 drillhole are a complex assemblage of Silurian, Devonian, and Mississippian sedimentary rocks that are bounded by numerous faults that show substantial stratigraphic offset. The final 7.3 m (24 ft) of this hole penetrated an unusual intrusive rock of Cretaceous age. The geology of this borehole was substantially different from that expected, with the Tongue Wash Fault encountered at a much shallower depth, paleozoic rocks shuffled out of stratigraphic sequence, and the presence of an altered biotite-rich microporphyritic igneous rock at the bottom of the borehole. Conodont CAI analyses and rock pyrolysis analyses indicate that the carbonate rocks in ER-12-1, as well as the intervening sheets of Eleana siltstone, have been thermally overprinted following movement on the faults that separate them. The probable source of heat for this thermal disturbance is the microporphyritic intrusion encountered at the bottom of the hole, and its age establishes that the major fault activity must have occurred prior to 102.3+0.5 Ma (middle Cretaceous).

  15. Microsoft Word - westwater_er.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2004 North Slope of Alaska Arctic Winter Radiometric Experiment: Overview and Recent Results E.R. Westwater, D. Cimini, M. Klein, and V. Leuski Cooperative Institute for Research in Environmental Sciences University of Colorado/National Oceanic and Atmospheric Administration - Earth System Research Boulder, Colorado V. Mattioli Dipartimento di Ingegneria Elettronica e dell'Informazione Università di Perugia Perugia, Italy A.J. Gasiewski Department of Electrical and Computer Engineering

  16. On the Use of an ER-213 Detonator to Establish a Baseline for the ER-486

    SciTech Connect (OSTI)

    Thomas, Keith A.; Liechty, Gary H.; Jaramillo, Dennis C.; Munger, Alan C.; McHugh, Douglas C.; Kennedy, James E.

    2014-08-19

    This report documents a series of tests using a TSD-115 fireset coupled with an ER-213, a gold exploding bridgewire (EBW) detonator. These tests were designed to fire this EBW with a smaller fireset to obtain current and voltage data as well as timing information at voltage levels below, above, and throughout the threshold firing region. This study could then create a database for comparison to our current ER-486 EBW development, which is designed to be a lower voltage (<500V) device.

  17. DOE/ER--0547T DE92

    Office of Scientific and Technical Information (OSTI)

    ER--0547T DE92 014826 II Chemical Contaminants on DOE Lands and Selection of Contaminant Mixtures for Subsurface Science Research R.G. Riley J.M. Zachara Pacific Northwest Laboratory In collaboration with F.J. Wobber April 1992 . L U.S. Department of Energy D_.:: :::;,:.::.::,;,_ <_'r._,_ L"_O'_UMf--N_ _S UNt.L,4,_L_ Office of Energy Research Subsurface Science Program W higt ....... -- aS n OI'I, U.L,. /..,UDOD 4_ This identifies individual contaminants and contaminant mixtures that

  18. DOE/ER/06035--7

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    06035--7 DE85 014321 Technical Progress Report SUPERSONIC METAL CLUSTER BEAMS Department of Ene'rgy Contract No. DE-AS0548ER06035 For Period 3-16-w through 4-l-85 Principal Investigator: R. E. Smalley DISCLAIMER This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their emptoyecs, makes any warranty, express or implied, or assumes any legal liability or rcspond- bility for

  19. K DOE/ER/72018~9

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    .^ay^4l.« XlUU..,^!^.:.^ K DOE/ER/72018~9 DE92 007472 Ninth Progress Report for the Division of Basic Energy Sciences Department of Energy, Contract DOE EY 76-S-03-0034, P.A. 218 (includes results of the last three years) MULTIHETEROMACROCYCLES THAT COMPLEX METAL IONS PRINCIPAL INVESTIGATOR: INSTITUTIONAL AFFILIATION: REPORTING PERIOD: DATE OF THIS REPORT: Donald J. Cram, Professor of Chemistry Department of Chemistry University of California at Los Angeles 405 Hilgard Avenue Los Angeles,

  20. Dali Yang er Hydropower Development Co Ltd | Open Energy Information

    Open Energy Info (EERE)

    Yang er Hydropower Development Co Ltd Jump to: navigation, search Name: Dali Yanger Hydropower Development Co Ltd Place: Dali Prefecture, Dali, Yunnan Province, China Zip: 625000...

  1. The new ternary pnictides Er{sub 12}Ni{sub 30}P{sub 21} and Er{sub

    Office of Scientific and Technical Information (OSTI)

    13}Ni{sub 25}As{sub 19}: Crystal structures and magnetic properties (Journal Article) | SciTech Connect The new ternary pnictides Er{sub 12}Ni{sub 30}P{sub 21} and Er{sub 13}Ni{sub 25}As{sub 19}: Crystal structures and magnetic properties Citation Details In-Document Search Title: The new ternary pnictides Er{sub 12}Ni{sub 30}P{sub 21} and Er{sub 13}Ni{sub 25}As{sub 19}: Crystal structures and magnetic properties The new ternary pnictides Er{sub 12}Ni{sub 30}P{sub 21} and Er{sub 13}Ni{sub

  2. DOE/ER/13897--9

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE/ER/13897--9 DE89 006617 THE BEHAVIOR OF MATTER UNDER NONEQUILIBRIUM CONDITIONS: FUNDAMENTAL ASPECTS AND APPLICATIONS P r o g r e s s Report A p r i l 1 5 , 1 9 8 8 - A p r i l 1 4 , 1989 3 -s 8 tf » § § *" l & s t l i g S S Hya Prigogine * s l S s l g o s S Center for Studies in S t a t i s t i c a l IVfechanics g -s g. "..§' S .g g S The University of Texas S I 2 g 0..8 j g - Austin, Texas 78712 *a " I'^l s 1 s I l i - S s ^ | S l o . Thisdocumentis ^ 0 o § 5 g c ^ l

  3. Final Technical Report for Award # ER64999

    SciTech Connect (OSTI)

    Metcalf, William W.

    2014-10-08

    This report provides a summary of activities for Award # ER64999, a Genomes to Life Project funded by the Office of Science, Basic Energy Research. The project was entitled "Methanogenic archaea and the global carbon cycle: a systems biology approach to the study of Methanosarcina species". The long-term goal of this multi-investigator project was the creation of integrated, multiscale models that accurately and quantitatively predict the role of Methanosarcina species in the global carbon cycle under dynamic environmental conditions. To achieve these goals we pursed four specific aims: (1) genome sequencing of numerous members of the Order Methanosarcinales, (2) identification of genomic sources of phenotypic variation through in silico comparative genomics, (3) elucidation of the transcriptional networks of two Methanosarcina species, and (4) development of comprehensive metabolic network models for characterized strains to address the question of how metabolic models scale with genetic distance.

  4. Green, red and infrared Er-related emission in implanted GaN:Er and GaN:Er,O samples

    SciTech Connect (OSTI)

    Monteiro, T.; Soares, J.; Correia, M. R.; Alves, E.

    2001-06-01

    Er-related luminescence near 1.54 {mu}m ({similar_to}805 meV) is observed under below band gap excitation at 4.2 K in GaN:Er and GaN:Er,O implanted samples. The spectrum of the recovered damage samples is a multiline structure. So far, these lines are the sharpest ones reported for GaN. Well-resolved green and red luminescences are observed in implanted samples. The dependence of luminescence on the excitation energy as well as the influence of different nominal fluence and annealing conditions is discussed. Combining the results obtained from photoluminescence and Rutherford backscattering spectrometry, different lattice sites for the optical active Er-related centers are identified. {copyright} 2001 American Institute of Physics.

  5. Coordination of Endoplasmic Reticulum (ER) Signaling During Maize Seed Development

    SciTech Connect (OSTI)

    Boston, Rebecca S.

    2010-11-20

    Seed storage reserves represent one of the most important sources of renewable fixed carbon and nitrogen found in nature. Seeds are well-adapted for diverting metabolic resources to synthesize storage proteins as well as enzymes and structural proteins needed for their transport and packaging into membrane bound storage protein bodies. Our underlying hypothesis is that the endoplasmic reticulum (ER) stress response provides the critical cellular control of metabolic flux required for optimal accumulation of storage reserves in seeds. This highly conserved response is a cellular mechanism to monitor the protein folding environment of the ER and restore homeostasis in the presence of unfolded or misfolded proteins. In seeds, deposition of storage proteins in protein bodies is a highly specialized process that takes place even in the presence of mutant proteins that no longer fold and package properly. The capacity of the ER to deposit these aberrant proteins in protein bodies during a period that extends several weeks provides an excellent model for deconvoluting the ER stress response of plants. We have focused in this project on the means by which the ER senses and responds to functional perturbations and the underlying intracellular communication that occurs among biosynthetic, trafficking and degradative pathways for proteins during seed development.

  6. ARM - Field Campaign - AERI-ER Intercomparison IOP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    govCampaignsAERI-ER Intercomparison IOP ARM Data Discovery Browse Data Comments? We would love to hear from you! Send us a note below or call us at 1-888-ARM-DATA. Send Campaign : AERI-ER Intercomparison IOP 2004.01.12 - 2006.06.29 Lead Scientist : David Turner Data Availability Data were collected and submitted to the ARM Archive for IOPs. For data sets, see below. Summary There were three, potentially four, phases to this experiment. The length of time required for each phase was the time

  7. Ozone induces glucose intolerance and systemic metabolic effects in young and aged brown Norway rats

    SciTech Connect (OSTI)

    Bass, V.; Gordon, C.J.; Jarema, K.A.; MacPhail, R.C.; Cascio, W.E.; Phillips, P.M.; Ledbetter, A.D.; Schladweiler, M.C.; Andrews, D.; Miller, D.; Doerfler, D.L.; Kodavanti, U.P.

    2013-12-15

    Air pollutants have been associated with increased diabetes in humans. We hypothesized that ozone would impair glucose homeostasis by altering insulin signaling and/or endoplasmic reticular (ER) stress in young and aged rats. One, 4, 12, and 24 month old Brown Norway (BN) rats were exposed to air or ozone, 0.25 or 1.0 ppm, 6 h/day for 2 days (acute) or 2 d/week for 13 weeks (subchronic). Additionally, 4 month old rats were exposed to air or 1.0 ppm ozone, 6 h/day for 1 or 2 days (time-course). Glucose tolerance tests (GTT) were performed immediately after exposure. Serum and tissue biomarkers were analyzed 18 h after final ozone for acute and subchronic studies, and immediately after each day of exposure in the time-course study. Age-related glucose intolerance and increases in metabolic biomarkers were apparent at baseline. Acute ozone caused hyperglycemia and glucose intolerance in rats of all ages. Ozone-induced glucose intolerance was reduced in rats exposed for 13 weeks. Acute, but not subchronic ozone increased ?{sub 2}-macroglobulin, adiponectin and osteopontin. Time-course analysis indicated glucose intolerance at days 1 and 2 (2 > 1), and a recovery 18 h post ozone. Leptin increased day 1 and epinephrine at all times after ozone. Ozone tended to decrease phosphorylated insulin receptor substrate-1 in liver and adipose tissues. ER stress appeared to be the consequence of ozone induced acute metabolic impairment since transcriptional markers of ER stress increased only after 2 days of ozone. In conclusion, acute ozone exposure induces marked systemic metabolic impairments in BN rats of all ages, likely through sympathetic stimulation. - Highlights: Air pollutants have been associated with increased diabetes in humans. Acute ozone exposure produces profound metabolic alterations in rats. Age influences metabolic risk factors in aging BN rats. Acute metabolic effects are reversible and repeated exposure reduces these effects. Ozone metabolic effects are only slightly exacerbated in geriatric rats.

  8. Pahute Mesa Well Development and Testing Analyses for Wells ER-20-7, ER-20-8 #2, and ER-EC-11, Revision 1

    SciTech Connect (OSTI)

    Greg Ruskauff

    2011-12-01

    This report analyzes the following data collected from ER-20-7, ER-20-8 No.2, and ER-EC-11 during WDT operations: (1) Chemical indicators of well development (Section 2.0); (2) Static hydraulic head (Section 3.0); (3) Radiochemistry and geochemistry (Section 4.0); (4) Drawdown observed at locations distal to the pumping well (Section 5.0); and (5) Drilling water production, flow logs, and temperature logs (Section 6.0). The new data are further considered with respect to existing data as to how they enhance or change interpretations of groundwater flow and transport, and an interim small-scale conceptual model is also developed and compared to Phase I concepts. The purpose of well development is to remove drilling fluids and drilling-associated fines from the formation adjacent to a well so samples reflecting ambient groundwater water quality can be collected, and to restore hydraulic properties near the well bore. Drilling fluids can contaminate environmental samples from the well, resulting in nonrepresentative measurements. Both drilling fluids and preexisting fines in the formation adjacent to the well can impede the flow of water from the formation to the well, creating artifacts in hydraulic response data measured in the well.

  9. QUARTER SH OR T-T ER M EN ER GY OU TL OO K QUAR TERL Y PROJ

    Gasoline and Diesel Fuel Update (EIA)

    1 2 QUARTER SH OR T-T ER M EN ER GY OU TL OO K QUAR TERL Y PROJ ECTIO NS ENERGY INFORMA TION ADMINIST RATION May 1991 This publication may be purchased from the Superintendent of Documents, U.S. Government Printing Office. Purchasing in formation for this or other Energy Information Administration (EIA) publications may be obtained from the Government Printing Office or ElA's National Energy Information Center. Questions on energy statistics should be directed to the Center by mail, telephone,

  10. Completion report for Well Cluster ER-20-6

    SciTech Connect (OSTI)

    1998-02-01

    The Well Cluster ER-20-6 drilling and completion project was conducted during February, March, and April of 1996 in support of the Nevada Environmental Restoration Project at the Nevada Test Site (NTS), Nye County, Nevada. This project is part of the DOE`s Underground Test Area (UGTA) subproject at the NTS. The primary UGTA tasks include collecting geological, geophysical, and hydrological data from new and existing wells to define groundwater quality as well as pathways and rates of groundwater migration at the NTS. A program of drilling wells near the sites of selected underground nuclear tests (near-field drilling) was implemented as part of the UGTA subproject to obtain site-specific data on the nature and extent of migration of radionuclides produced by an underground nuclear explosion. The ER-20-6 near-field drilling project was originally planned to be very similar to that recently conducted at Well Cluster ER-20-5, which was designed to obtain data on the existing hydrologic regime near the site of an underground nuclear explosion (IT, 1995; IT, 1996a). However, after further consideration of the goals of the near-field drilling program and the characteristics of the BULLION site, the TWG recommended that the ER-20-6 project be redesigned to accommodate a forced-gradient experiment. This proposed experiment is expected to yield more realistic estimates of transport parameters than can be deduced from sampling and testing natural groundwater flow systems.

  11. Inner Mongolia Bayannao er Fuhui Wind Power Co Ltd | Open Energy...

    Open Energy Info (EERE)

    Bayannao er Fuhui Wind Power Co Ltd Jump to: navigation, search Name: Inner Mongolia Bayannao'er Fuhui Wind Power Co Ltd Place: Inner Mongolia Autonomous Region, China Sector: Wind...

  12. Bosch Solar Energy AG former ErSol Solar Energy AG | Open Energy...

    Open Energy Info (EERE)

    AG former ErSol Solar Energy AG Jump to: navigation, search Name: Bosch Solar Energy AG (former ErSol Solar Energy AG) Place: Erfurt, Germany Zip: D-99099 Product: Germany-based...

  13. USACE ER 200-2-2 Procedures for Implementing NEPA | Open Energy...

    Open Energy Info (EERE)

    ER 200-2-2 Procedures for Implementing NEPA Jump to: navigation, search OpenEI Reference LibraryAdd to library PermittingRegulatory Guidance - GuideHandbook: USACE ER 200-2-2...

  14. Audit Report: ER-B-99-05 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Audit Report: ER-B-99-05 April 8, 1999 Westinghouse Savannah River Company's Withdrawal ... (FY) 1997 and 1998. PDF icon Audit Report: ER-B-99-05 More Documents & Publications Audit ...

  15. Audit Report: ER-B-97-03 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Audit Report: ER-B-97-03 June 5, 1997 Audit of Proposal to Acquire Land at the Fernald Environmental Management Project PDF icon Audit Report: ER-B-97-03 More Documents & ...

  16. Audit Report: ER-B-98-03 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 Audit Report: ER-B-98-03 November 7, 1997 Audit of the Union Valley Sample Preparation ... and cost effective. PDF icon Audit Report: ER-B-98-03 More Documents & Publications Audit ...

  17. Audit Report: ER-B-98-09 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9 Audit Report: ER-B-98-09 July 15, 1998 Disposal of Tritium Residues at the Los Alamos ... PDF icon Audit Report: ER-B-98-09 More Documents & Publications Semiannual Report to ...

  18. Audit Report: ER-B-96-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 Audit Report: ER-B-96-01 April 23, 1996 Force Restructuring at the Fernald Environmental Management Project PDF icon Audit Report: ER-B-96-01 More Documents & Publications Audit ...

  19. Audit Report: ER-B-97-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ER-B-97-01 October 22, 1996 Audit of Economic Development Grants and a Cooperative Agreement with East Tennessee Not-For-Profit Organizations PDF icon Audit Report: ER-B-97-01 More ...

  20. Audit Report: ER-B-98-04 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Audit Report: ER-B-98-04 November 24, 1997 Audit Of Selected Government-Funded Grants ... PDF icon Audit Report: ER-B-98-04 More Documents & Publications Audit Report: IG-0524 ...

  1. Audit Report: ER-B-98-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 Audit Report: ER-B-98-01 October 23, 1997 Audit of the Deactivation, Decontamination, ... PDF icon Audit Report: ER-B-98-01 More Documents & Publications Audit Report: IG-0684 ...

  2. Audit Report: ER-B-95-06 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Audit Report: ER-B-95-06 August 3, 1995 Audit of Work Force Restructuring at the Oak Ridge Operations Office PDF icon Audit Report: ER-B-95-06 More Documents & Publications Audit ...

  3. Audit Report: ER-B-95-04 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    4 Audit Report: ER-B-95-04 June 26, 1995 Audit of the Replacement High Level Waste Evaporator at Savannah River PDF icon Audit Report: ER-B-95-04 More Documents & Publications ...

  4. Final Technical Report for DOE DE-FG02-05ER54831 "Laboratory...

    Office of Scientific and Technical Information (OSTI)

    DOE DE-FG02-05ER54831 "Laboratory Studies of Dynamos." Citation Details In-Document Search Title: Final Technical Report for DOE DE-FG02-05ER54831 "Laboratory Studies of Dynamos." ...

  5. Audit Report: ER-B-98-08 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8 Audit Report: ER-B-98-08 May 29, 1998 The Cost Reduction Incentive Program at the ... of the CRIP. PDF icon Audit Report: ER-B-98-08 More Documents & Publications Audit ...

  6. Audit Report: ER-B-96-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 Audit Report: ER-B-96-02 June 11, 1996 Audit of Groundwater Remediation Plans at Savannah River PDF icon Audit Report: ER-B-96-02 More Documents & Publications EIS-0120: Wetland ...

  7. Audit Report: ER-B-98-07 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7 Audit Report: ER-B-98-07 April 6, 1998 Personal Property at the Oak Ridge Operations ... PDF icon Audit Report: ER-B-98-07 More Documents & Publications Audit Report: IG-0664 ...

  8. Audit Report: ER-B-00-03 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 Audit Report: ER-B-00-03 June 19, 2000 Waste Characterization at Oak Ridge Waste ... PDF icon Audit Report: ER-B-00-03 More Documents & Publications Audit Report: IG-0434 ...

  9. Audit Report: ER-L-02-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    L-02-01 Audit Report: ER-L-02-01 February 7, 2002 The Department of Energy's Strategy for ... PDF icon Audit Report: ER-L-02-01 More Documents & Publications Audit Report: IG-0688 ...

  10. Audit Report: ER-B-99-08 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8 Audit Report: ER-B-99-08 May 12, 1999 Health Physics Technician Subcontracts at ... PDF icon Audit Report: ER-B-99-08 More Documents & Publications Semiannual Report to ...

  11. Audit Report: ER-B-95-05 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Audit Report: ER-B-95-05 July 14, 1995 Audit of Acquisition of Scientific Research at Ames Laboratory PDF icon Audit Report: ER-B-95-05 More Documents & Publications Audit ...

  12. Audit Report: ER-B-99-03 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3 Audit Report: ER-B-99-03 January 25, 1999 Westinghouse Savannah River Company's Health ... January 1, 1997. PDF icon Audit Report: ER-B-99-03 More Documents & Publications ...

  13. Audit Report: ER-B-99-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    9-01 Audit Report: ER-B-99-01 December 21, 1998 Decontamination and Decommissioning at the ... at the ETTP. PDF icon Audit Report: ER-B-99-01 More Documents & Publications Audit ...

  14. Audit Report: ER-B-98-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 Audit Report: ER-B-98-02 October 24, 1997 Audit of Environmental Monitoring and Health ... PDF icon Audit Report: ER-B-98-02 More Documents & Publications Audit Report: IG-0522 ...

  15. Audit Report: ER-B-00-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 Audit Report: ER-B-00-02 June 21, 2000 Security Overtime at the Oak Ridge Operations ... PDF icon Audit Report: ER-B-00-02 More Documents & Publications Audit Report: IG-0487 ...

  16. Magnetic properties of Er1-xDyxAl2 (0 ≤ x ≤ 1) compounds...

    Office of Scientific and Technical Information (OSTI)

    of Er1-xDyxAl2 (0 x 1) compounds in low applied fields Citation Details In-Document Search Title: Magnetic properties of Er1-xDyxAl2 (0 x 1) compounds in ...

  17. Audit Report: ER-B-98-05 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5 Audit Report: ER-B-98-05 December 10, 1997 Audit Of The Department Of Energy's Contracts ... PDF icon Audit Report: ER-B-98-05 More Documents & Publications Audit Report: IG-0426 ...

  18. Exposure of Jurkat cells to bis (tri-n-butyltin) oxide (TBTO) induces transcriptomics changes indicative for ER- and oxidative stress, T cell activation and apoptosis

    SciTech Connect (OSTI)

    Katika, Madhumohan R.; Hendriksen, Peter J.M.; Loveren, Henk van; Peijnenburg, Ad

    2011-08-01

    Tributyltin oxide (TBTO) is an organotin compound that is widely used as a biocide in agriculture and as an antifouling agent in paints. TBTO is toxic for many cell types, particularly immune cells. The present study aimed to identify the effects of TBTO on the human T lymphocyte cell line Jurkat. Cells were treated with 0.2 and 0.5 {mu}M TBTO for 3, 6, 12 and 24 h and then subjected to whole genome gene expression microarray analysis. The biological interpretation of the gene expression profiles revealed that endoplasmic reticulum (ER) stress is among the earliest effects of TBTO. Simultaneously or shortly thereafter, oxidative stress, activation of NFKB and NFAT, T cell activation, and apoptosis are induced. The effects of TBTO on genes involved in ER stress, NFAT pathway, T cell activation and apoptosis were confirmed by qRT-PCR. Activation and nuclear translocation of NFATC1 and the oxidative stress response proteins NRF2 and KEAP1 were confirmed by immunocytology. Taking advantage of previously published microarray data, we demonstrated that the induction of ER stress, oxidative stress, T cell activation and apoptosis by TBTO is not unique for Jurkat cells but does also occur in mouse thymocytes both ex vivo and in vivo and rat thymocytes ex vivo. We propose that the induction of ER stress leading to a T cell activation response is a major factor in the higher sensitivity of immune cells above other types of cells for TBTO. - Research Highlights: > The human T lymphocyte cell line Jurkat was exposed to TBTO. > Whole-genome microarray experiments were performed. > Data analysis revealed the induction of ER stress and activation of NFAT and NFKB. > Exposure to TBTO also led to T cell activation, oxidative stress and apoptosis.

  19. Audit Report: ER-B-96-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Audit Report: ER-B-96-02 Audit Report: ER-B-96-02 June 11, 1996 Audit of Groundwater Remediation Plans at Savannah River PDF icon Audit Report: ER-B-96-02 More Documents & Publications Audit Report: IG-0655 EIS-0120: Wetland Assessment EIS-0303: Draft Environmental Impact Statement

  20. Audit Report: ER-B-98-06 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8-06 Audit Report: ER-B-98-06 April 1, 1998 Fluor Daniel Fernald's Use of Temporary ... PDF icon Audit Report: ER-B-98-06 More Documents & Publications Audit Report: ER-B-96-01 ...

  1. Final Report DE-FG02-07ER64416

    SciTech Connect (OSTI)

    Seymour, Joseph D.

    2014-02-01

    The document provides the Final Report for DE-FG02-07ER64416 on the use of magnetic resonance (MR) methods to quantify transport in porous media impacted by biological and chemical processes. Products resulting from the research in the form of peer reviewed publications and conference presentations are presented. The research correlated numerical simulations and MR measurements to test simulation methodology. Biofilm and uranium detection by MR was demonstrated.

  2. ER85357_Phase2_Eltron | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Unconventional High Temperature Nanofiltration for Produced Water Treatment Last Reviewed 6/25/2013 DE-10ER85357 Goal The project goal is to further develop a proprietary, high-temperature nanofiltration (NF) technology (DurafluxTM) to remove salt and other dissolved solids from produced water originating from domestic oil and gas production. Treated water can be re-used in the extraction process without cooling/re-heating costs or can be recycled as an acceptable supply of source water. Project

  3. Completion Report for Well Cluster ER-6-1

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-10-01

    Well Cluster ER-6-1 was constructed for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Division at the Nevada Test Site, Nye County, Nevada. This work was initiated as part of the Groundwater Characterization Project, now known as the Underground Test Area Project. The well cluster is located in southeastern Yucca Flat. Detailed lithologic descriptions with stratigraphic assignments for Well Cluster ER-6-1 are included in this report. These are based on composite drill cuttings collected every 3 meters and conventional core samples taken below 639 meters, supplemented by geophysical log data. Detailed petrographic, chemical, and mineralogical studies of rock samples were conducted on 11 samples to resolve complex interrelationships between several of the Tertiary tuff units. Additionally, paleontological analyses by the U.S. Geological Survey confirmed the stratigraphic assignments below 539 meters within the Paleozoic sedimentary section. All three wells in the Well ER-6-1 cluster were drilled within the Quaternary and Tertiary alluvium section, the Tertiary volcanic section, and into the Paleozoic sedimentary section.

  4. Completion Report for Well Cluster ER-5-4

    SciTech Connect (OSTI)

    U.S. Department of Energy, National Nuclear Security Administration, Nevada Site Office; Bechtel Nevada

    2005-02-01

    Well Cluster ER-5-4 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The cluster consists of two wells, positioned about 30 meters apart on the same drill pad, constructed as part of a hydrogeologic investigation program for Frenchman Flat at the Nevada Test Site. Detailed lithologic descriptions with preliminary stratigraphic assignments for the well cluster are included in this report. These are based on composite drill cuttings collected every 3 meters, and 156 sidewall samples taken at various depths below 192 meters in both boreholes, supplemented by geophysical log data. Detailed petrographic, chemical, and mineralogical studies of rock samples were conducted on 122 samples. Well ER-5-4 penetrated approximately 1,120 meters of Quaternary and Tertiary alluvium before reaching total depth in Tertiary volcanic rocks at 1,137.5 meters. The deeper Well ER-5-4 No.2 penetrated 1,120.4 meters of alluvial sediments, and was terminated within Tertiary volcanic rocks at a depth of 2,133.6 meters, indicating that Paleozoic rocks are deeper than expected at this site.

  5. Tunable, diode side-pumped Er:YAG laser

    DOE Patents [OSTI]

    Hamilton, C.E.; Furu, L.H.

    1997-04-22

    A discrete-element Er:YAG laser, side pumped by a 220 Watt peak-power InGaAs diode array, generates >500 mWatts at 2.94 {micro}m, and is tunable over a 6 nm range near about 2.936 {micro}m. The oscillator is a plano-concave resonator consisting of a concave high reflector, a flat output coupler, a Er:YAG crystal and a YAG intracavity etalon, which serves as the tuning element. The cavity length is variable from 3 cm to 4 cm. The oscillator uses total internal reflection in the Er:YAG crystal to allow efficient coupling of the diode emission into the resonating modes of the oscillator. With the tuning element removed, the oscillator produces up to 1.3 Watts of average power at 2.94 {micro}m. The duty factor of the laser is 6.5% and the repetition rate is variable up to 1 kHz. This laser is useful for tuning to an atmospheric transmission window at 2.935 {micro}m (air wavelength). The laser is also useful as a spectroscopic tool because it can access several infrared water vapor transitions, as well as transitions in organic compounds. Other uses include medical applications (e.g., for tissue ablation and uses with fiber optic laser scalpels) and as part of industrial effluent monitoring systems. 4 figs.

  6. Tunable, diode side-pumped Er: YAG laser

    DOE Patents [OSTI]

    Hamilton, Charles E.; Furu, Laurence H.

    1997-01-01

    A discrete-element Er:YAG laser, side pumped by a 220 Watt peak-power InGaAs diode array, generates >500 mWatts at 2.94 .mu.m, and is tunable over a 6 nm range near about 2.936 .mu.m. The oscillator is a plano-concave resonator consisting of a concave high reflector, a flat output coupler, a Er:YAG crystal and a YAG intracavity etalon, which serves as the tuning element. The cavity length is variable from 3 cm to 4 cm. The oscillator uses total internal reflection in the Er:YAG crystal to allow efficient coupling of the diode emission into the resonating modes of the oscillator. With the tuning element removed, the oscillator produces up to 1.3 Watts of average power at 2.94 .mu.m. The duty factor of the laser is 6.5% and the repetition rate is variable up to 1 kHz. This laser is useful for tuning to an atmospheric transmission window at 2.935 .mu.m (air wavelength). The laser is also useful as a spectroscopic tool because it can access several infrared water vapor transitions, as well as transitions in organic compounds. Other uses include medical applications (e.g., for tissue ablation and uses with fiber optic laser scalpels) and as part of industrial effluent monitoring systems.

  7. X-ray and neutron diffraction of Er-hydride films.

    SciTech Connect (OSTI)

    Rodriguez, Mark Andrew

    2004-10-01

    The outline of this report is: (1) structures of hexagonal Er meal, ErH{sub 2} fluorite, and molybdenum; (2) texture issues and processing effects; (3) idea of pole figure integration; and (4) promising neutron diffraction work. Summary of this report are: (1) ErD{sub 2} and ErT{sub 2} film microstructures are strongly effected by processing conditions; (2) both x-ray and neutron diffraction are being pursued to help diagnose structure/property issues regarding ErT{sub 2} films and these correlations to He retention/release; (3) texture issues are great challenges for determination of site occupancy; and (4) work on pole-figure-integration looks to have promise addressing texture issues in ErD{sub 2} and ErT{sub 2} films.

  8. COMPLETION REPORT FOR WELL CLUSTER ER-5-3

    SciTech Connect (OSTI)

    BECHTEL NEVADA

    2005-12-01

    Well Cluster ER-5-3 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This cluster of 3 wells was drilled in 2000 and 2001 as part of a hydrogeologic investigation program in Frenchman Flat. The first borehole in the cluster, Well ER-5-3, was drilled in February and March 2000. A 47.0-centimeter surface hole was drilled and cased off to the depth of 374.8 meters. The hole diameter was decreased to 31.1 centimeters for drilling to a total depth of 794.3 meters within welded ash-flow tuff. A piezometer string with 1 slotted interval was installed in the annulus of the surface casing, open to the saturated alluvium. A completion string with 2 slotted intervals was installed in the main hole, open to saturated alluvium and to the welded tuff aquifer. A second piezometer string with 1 slotted interval open to the welded-tuff aquifer was installed outside the completion string. Well ER-5-3 No.2 was drilled about 30 meters west of the first borehole in March 2000, and was recompleted in March 2001. A 66.0-centimeter hole was drilled and cased off to the depth of 613.8 meters. The hole diameter was decreased to 44.5 centimeters and the borehole was drilled and cased off to the depth of 849.0 meters. The hole diameter was decreased once more to 31.1 centimeters for drilling to a total depth of 1,732.2 meters in dolomite. A completion string open to the dolomite (lower carbonate aquifer) was installed. Well ER-5-3 No.3 was drilled approximately 30 meters north of the first 2 boreholes in February 2001. A 66.0-centimeter hole was drilled and cased off to the depth of 36.6 meters, then the main 25.1-centimeter-diameter hole was drilled to a total depth of 548.6 meters in alluvium. A slotted stainless-steel tubing string was installed in the saturated alluvium. A preliminary composite, static water level was measured at the depth of 282.6 meters, prior to development and hydrologic testing. Detailed lithologic descriptions and stratigraphic assignments are included in the report. These are based on composite drill cuttings collected every 3 meters, and 120 sidewall samples taken at various depths below 91 meters in Wells ER-5-3 and ER-5-3 No.2, supplemented by geophysical log data. The wells penetrated Quaternary/Tertiary alluvium to the depth of 622.4 meters, and an 8.5-meter-thick basalt flow was encountered within the alluvium. Tertiary tuff was penetrated to the depth of approximately 1,425.9 meters, where the top of the lower carbonate aquifer was tagged in Well ER-5-3 No.2.

  9. CNSS Papers CI Siegfried S. Hecl^er

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    CNSS Papers CI Siegfried S. Hecl^er m Center for National Security Studies Los Alamos National Laboratory 0!STffl?UlSUrf J? THIS iiOCy^fttMi cS l-^LI^iTEfi CENTER FOR NATIONAL SECURITY STUDIES The Center for National Security Studies is a studies and analysis or- ganization of the Los Alamos National Laboratory. Drawing on the broad knowledge at Los Alamos of science and engineering relevant to national security issues, the Center's research focuses on the interaction between technology and

  10. Final Technical Report for Award # ER64999 (Technical Report) | SciTech

    Office of Scientific and Technical Information (OSTI)

    Connect Technical Report: Final Technical Report for Award # ER64999 Citation Details In-Document Search Title: Final Technical Report for Award # ER64999 This report provides a summary of activities for Award # ER64999, a Genomes to Life Project funded by the Office of Science, Basic Energy Research. The project was entitled "Methanogenic archaea and the global carbon cycle: a systems biology approach to the study of Methanosarcina species". The long-term goal of this

  11. Completion Report for Well ER-8-1

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-11-01

    Well ER-8-1 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in October and November of 2002 as part of a Hydrogeologic investigation program for the Yucca Flat/Climax Mine Corrective Action Unit in the northeastern portion of the Nevada Test Site. Well ER-8-1 is located at the north end of Yucca Flat approximately 580 meters south-southeast of the surface exposure of the Climax granitic intrusive. Detailed lithologic descriptions with stratigraphic assignments are included in this report. These are based on composite drill cuttings samples collected every 3 meters, and 21 sidewall samples taken at various depths between 351.1 and 573.0 meters, supplemented by incomplete geophysical log data. Detailed petrographic, geochemical, and mineralogical studies of rock samples were conducted on 22 samples of drill cuttings. Drilling began in tuffaceous alluvium, and the borehole penetrated Tertiary age bedded tuffs of the Volcanics of Oak Spring Butte and carbonate sediments of Paleozoic age, which were encountered at a depth of 334 meters. The borehole unexpectedly penetrated granite at the depth of 538.9 meters in which drilling was stopped. Contact metamorphic rocks and intrusive dikes associated with the Cretaceous-age granitic intrusive and at least one significant fault zone were encountered.

  12. Completion Report for Well ER-2-1

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-10-01

    Well ER-2-1 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office (formerly Nevada Operations Office), in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in February and March of 2003, as part of a hydrogeologic investigation program for the Yucca Flat/Climax Mine Corrective Action Unit in the northeastern portion of the Nevada Test Site. Well ER-2-1 was drilled as part of the Yucca Flat Corrective Action Unit Phase I drilling initiative. The well is located in north central Yucca Flat within Area 2 of the Nevada Test Site, and provided information regarding the radiological and physical environment near underground nuclear tests conducted in a saturated volcanic aquifer setting. Detailed lithologic descriptions with stratigraphic assignments are included in this report. These are based on composite drill cuttings collected every 3 meters and 83 sidewall samples taken at various depths between 113.7 and 754.4 meters, supplemented by geophysical log data. Detailed petrographic, chemical, and mineralogical studies of rock samples were conducted on 27 samples of drill cuttings. The well was collared in tuffaceous alluvium, and penetrated Tertiary-age tuffs of the Timber Mountain and Paintbrush Groups, Calico Hills and Wahmonie Formations, Crater Flat Group, Grouse Canyon Formation, before reaching total depth in the Tunnel Bed Formation.

  13. ER Consolidated Qtrly Rpt_July-September 2015_January 2016

    SciTech Connect (OSTI)

    Cochran, John R.

    2016-01-01

    This Environmental Restoration Operations (ER) Consolidated Quarterly Report (ER Quarterly Report) provides the status of ongoing corrective action activities being implemented by Sandia National Laboratories, New Mexico (SNL/NM) for the July, August, and September 2015 quarterly reporting period. The Solid Waste Management Units (SWMUs) and Areas of Concern (AOCs) identified for corrective action at SNL/NM are listed in Table I-1. The work completed during this quarter is reported below in Sections I.2.1 and I.2.2. Section I.2.1 summarizes the quarterly activities at sites undergoing corrective action field activities (SWMUs 8 and 58, 68, 149, 154, and 502, and three groundwater AOCs). Section I.2.2 summarizes quarterly activities at sites where the New Mexico Environment Department (NMED) has issued a certificate of completion and the site is in the corrective action complete (CAC) regulatory process. Currently, the Mixed Waste Landfill (MWL, SWMU 76) is the only site in the CAC regulatory process. Corrective action activities have been deferred at the Long Sled Track (SWMU 83), the Gun Facilities (SWMU 84), and the Short Sled Track (SWMU 240) because these are active mission facilities.

  14. Hydrothermal synthesis and magnetic properties of ErCrO{sub 4} nanoparticles

    SciTech Connect (OSTI)

    Sundarayya, Y. Kumar, K. Ashwini Sondge, Rajesh Srinath, S. Kaul, S. N.

    2014-04-24

    Homogeneous single phase ErCrO{sub 4} nanoparticles have been synthesized by a modified sol-gel followed by hydrothermal method. X-ray diffraction reveals that the compound crystallizes into tetragonal structure with space group I41/amd. The average crystallite size was estimated to be 21(1) nm. Morphological analysis of the sample confirms uniform particles of size 20 nm. DC magnetic measurements show that ErCrO{sub 4} undergoes a paramagnetic-antiferromagnetic transition at 16 K, due to the superexchange Er-O-Cr-O-Er antiferromagnetic interactions.

  15. Environmental Release Summary (ERS) database CY 1995 releases and supporting data

    SciTech Connect (OSTI)

    Gleckler, B.P., Westinghouse Hanford

    1996-08-05

    This document is a hard copy of the CY 1995 airborne and liquid effluent data contained in the Environmental Release Summary (ERS) computer database.

  16. Completion Report for Well ER-18-2

    SciTech Connect (OSTI)

    Bechtel Nevada

    2003-09-01

    Well ER-18-2 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well, located on Buckboard Mesa in the western part of the Nevada Test Site, was drilled in the spring of 1999 as part of the U.S. Department of Energy's hydrogeologic investigation well program in the Western Pahute Mesa - Oasis Valley region just west of the Test Site. A 44.5-centimeter surface hole was drilled and cased off to the depth 408.1 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 762.0 meters. A preliminary composite, static, water level was measured at the depth of approximately 369.7 meters approximately two months after the completion string was installed. One completion string with three isolated, slotted intervals was installed in the well. Detailed lithologic descriptions with preliminary stratigraphic assignments are included in the report. These are based on composite drill cuttings collected every 3 meters and 15 sidewall samples taken at various depths below 420 meters, supplemented by geophysical log data and results of detailed chemical and mineralogical studies of rock samples. The upper part of the well penetrated Tertiary-age basalt, underlain by tuffaceous moat-filling sediments interbedded with ash-flow tuff units of the Thirsty Canyon Group and the Beatty Wash Formation. The lower half of the drill hole penetrated ash-flow tuff of the mafic-rich Ammonia Tanks Tuff. The geologic interpretation of data from Well ER-18-2 indicates that this site is located inside the structural margin of the Ammonia Tanks caldera.

  17. Completion Report for Well ER-EC-1

    SciTech Connect (OSTI)

    Townsend, M.J.

    2000-12-01

    Well ER-EC-1 was drilled for the U.S. Department of Energy, Nevada Operations Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in the spring of 1999 as part of the U.S. Department of Energy's hydrogeologic investigation well program in the Western Pahute Mesa - Oasis Valley region just west of the Test Site. A 44.5-centimeter surface hole was drilled and cased off to the depth 675.1 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 1,524.0 meters. A preliminary composite, static, water level was measured at the depth of approximately 566.3 meters prior to installation of the completion string. One completion string with three isolated, slotted intervals was installed in the well. Detailed lithologic descriptions with preliminary stratigraphic assignments are included in the report. These are based on composite drill cuttings collected every 3 meters and 31 sidewall samples taken at various depths below 680 meters, supplemented by geophysical log data. Detailed chemical and mineralogical studies of rock samples are in progress. The well penetrated Tertiary-age lava and tuff of the Timber Mountain Group, the Paintbrush Group, the Calico Hills Formation, the Crater Flat Group, and the Volcanics of Quartz Mountain. The preliminary geologic interpretation of data from Well ER-EC-1 indicates the presence of a structural trough or bench filled with a thick section of post-Rainier Mesa lava. These data also suggest that this site is located on a buried structural ridge that may separate the Silent Canyon and Timber Mountain caldera complexes.

  18. Completion Report for Well ER-7-1

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-11-01

    Well ER-7-1 was drilled for the U.S. Department of Energy's National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in January and February 2003, as part of a hydrogeologic investigation program in Yucca Flat. A 47.0-centimeter surface hole was drilled and cased off to a depth of 541.0 meters below the surface. The hole diameter was then decreased to 31.8 centimeters for drilling to a total depth of 762.0 meters. Detailed lithologic descriptions with stratigraphic assignments are included in this report. These are based on composite drill cuttings collected every 3 meters, and 62 sidewall samples taken at various depths below 85.3 meters, supplemented by geophysical log data. Detailed petrographic, chemical, and mineralogical studies were conducted on 22 samples of cuttings. The well was collared in Quaternary surficial deposits and penetrated a thick section of Tertiary-age volcanic deposits before terminating in carbonate rocks of Paleozoic-age.

  19. Completion Report for Wells ER-20-8 and ER-20-8#2 Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2011-02-28

    Wells ER-20-8 and ER-20-8#2 were drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada National Security Site (formerly Nevada Test Site), Nye County, Nevada. The holes were drilled in July and August 2009, as part of the Pahute Mesa Phase II drilling program. The primary purpose of these wells was to provide detailed hydrogeologic information in the Tertiary volcanic section that will help address uncertainties within the Pahute MesaOasis Valley hydrostratigraphic framework model. They may also be used as long-term monitoring wells.

  20. Audit Report: ER-B-99-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    submit offers. PDF icon Audit Report: ER-B-99-02 More Documents & Publications Microsoft Word - al2004-03.doc Chapter 19 - Small Business Programs Acquisition Letter: AL2005-08...

  1. Audit Report: ER-B-99-06 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 Audit Report: ER-B-99-06 April 14, 1999 Bechtel Jacobs Payroll Creation The Oak Ridge Operations Office (Operations Office) awarded a contract to the Bechtel Jacobs Company, LLC...

  2. Audit Report: ER-B-99-07 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    7 Audit Report: ER-B-99-07 May 4, 1999 Maintenance Activities at the Y-12 Plant Department of Energy (Department) policy requires the use of performance measures to assess the...

  3. Audit Report: ER-B-98-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Audit Report: ER-B-98-02 October 24, 1997 Audit of Environmental Monitoring and Health Physics Laboratories at the Savannah River Site The Environmental Monitoring and Health...

  4. Completion Report for Well ER-EC-5

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-10-01

    Well ER-EC-5 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in the summer of 1999 as part of the U.S. Department of Energy's hydrogeologic investigation program in the Western Pahute Mesa - Oasis Valley region just west of the Nevada Test Site. A 44.5-centimeter surface hole was drilled and cased off to a depth of 342.6 meters below ground surface. The borehole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 762.0 meters. One completion string with three isolated slotted intervals was installed in the well. A preliminary composite, static water level was measured at the depth of 309.9 meters, 40 days after installation of the completion string. Detailed lithologic descriptions with stratigraphic assignments are included in the report. These are based on composite drill cuttings collected every 3 meters, and 18 sidewall samples taken at various depths below 349.6 meters, supplemented by geophysical log data and results from detailed chemical and mineralogical analyses of rock samples. The well penetrated Tertiary-age tuffs of the Thirsty Canyon Group, caldera moat-filling sedimentary deposits, lava of the Beatty Wash Formation, and landslide breccia and tuffs of the Timber Mountain Group. The well reached total depth in welded ashflow tuff of the Ammonia Tanks Tuff after penetrating 440.1 meters of this unit, which is also the main water-producing unit in the well. The geologic interpretation of data from this well constrains the western margin of the Ammonia Tanks caldera to the west of the well location.

  5. Completion Report for Well ER-12-2

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-11-01

    Well ER-12-2 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The well was drilled from November 2002 to January 2003 as part of a hydrogeologic investigation program for the Yucca Flat Corrective Action Unit. The overall purpose of the well was to gather subsurface data to better characterize the hydrogeology in the northwestern portion of Yucca Flat. The well was drilled to total measured depth of 2,097.9 meters. The 131.1-centimeter-diameter borehole was left open (i.e., uncased) below the base of the intermediate casing at 901.6 meters. A piezometer string was installed outside the surface casing to a depth of 176.4 meters to monitor a zone of perched water. Data gathered during and shortly after hole construction include composite drill cuttings samples collected every 3 meters, sidewall core samples from 7 depths, various geophysical logs, and water level measurements. These data indicate that the well penetrated, in descending order, 137.5 meters of Quaternary and Tertiary alluvium, 48.8 meters of Tertiary volcanic rocks, 289.6 meters of Mississippian Chainman Shale, and 1,622.5 meters of Mississippian and Upper Devonian Eleana Formation consisting of shale, argillite, sandstone, quartzite, and limestone. Forty-seven days after the well was drilled the water level inside the main hole was tagged at the depth of 65.43 meters, and the water level inside the piezometer string was tagged at 127.14 meters.

  6. Completion Report for Well ER-EC-2A

    SciTech Connect (OSTI)

    M. J. Townsend

    2002-03-01

    Well ER-EC-2A was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Operations Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in January and February of 2000 as part of a hydrogeologic investigation program in the Pahute Mesa - Oasis Valley region just west of the Nevada Test Site. A 44.5-centimeter surface hole was drilled and cased off to a depth of 412.9 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 1,516.1 meters. One completion string with three isolated slotted intervals was installed in the well. A preliminary composite, static water level was measured at the depth of 228.0 meters, approximately two months after installation of the completion string. Detailed lithologic descriptions with preliminary stratigraphic assignments are included in this report. These are based on composite drill cuttings collected every 3 meters, and 81 sidewall samples taken at various depths below 212 meters, supplemented by geophysical log data. Detailed petrographic, chemical, and mineralogical studies of rock samples were conducted on 30 samples. The well was collared in rhyolite lava and penetrated Tertiary-age lava and tuff of the Volcanics of Fortymile Canyon and the Timber Mountain Group. The preliminary geologic interpretation of borehole data indicates that this well was drilled within the margins of the buried Rainier Mesa and Ammonia Tanks calderas, and that caldera collapse in this area was deeper than expected, resulting in a section of Volcanics of Fortymile Canyon (caldera-filling deposit) that is much thicker than expected.

  7. Completion Report for Well ER-EC-4

    SciTech Connect (OSTI)

    M. J. Townsend

    2000-09-01

    Well ER-EC-4 was drilled for the US Department of Energy, Nevada Operations Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in the summer of 1999 as part of the U.S Department of Energy's hydrogeologic investigation well program in the Western Pahute Mesa - Oasis Valley region just west of the Test Site. A 44.5-centimeter surface hole was drilled and cased off to a depth of 263.7 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 1,062.8 meters. One completion string with three isolated slotted intervals was installed in the well. A preliminary composite, static, water level was measured at the depth of 228.3 meters, two months after installation of the completion string. Detailed lithologic descriptions with preliminary stratigraphic assignments are included in the report. These are based on composite drill cuttings collected every 3 meters, and 35 sidewall samples taken at various depths below 286.5 meters, supplemented by geophysical log data. Detailed chemical and mineralogical studies of rock samples are in progress. The well was collared in basalt and penetrated Tertiary-age lava and tuff of the Thirsty Canyon Group, the Volcanics of Fortymile Canyon, and the Timber Mountain Group. The preliminary geologic interpretation of data from this well helps pinpoint the location of the western margin of the Timber Mountain caldera complex in the southern Nevada volcanic field.

  8. Completion Report for Well ER-EC-7

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-10-01

    Well ER-EC-7 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in the summer of 1999 as part of the Department of Energy's hydrogeologic investigation program in the Western Pahute Mesa - Oasis Valley region just west of the Test Site. A 44.5-centimeter surface hole was drilled and cased off to a depth of 265.8 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 422.5 meters. The planned depth of 762 meters was not reached due to borehole stability problems. One completion string with two isolated slotted intervals was installed in the well. A preliminary composite, static, water level was measured at the depth of 227.8 meters, 20 days after installation of the completion string. Detailed lithologic descriptions with stratigraphic assignments are included in the report. These are based on composite drill cuttings, supplemented by geophysical log data, and incorporating data from detailed chemical and mineralogical studies of rock samples. Beneath a thin alluvial deposit, the well penetrated 410 meters of lava and bedded tuff of the Volcanics of Fortymile Canyon Group, deposited in the Timber Mountain caldera moat after caldera collapse. The geologic interpretation of data from this well provides information on the thickness, lithologic composition, and hydrogeologic character of moat-filling rocks in the southern portion of the Timber Mountain caldera complex in the southwestern Nevada volcanic field.

  9. Completion report for Well ER-EC-6

    SciTech Connect (OSTI)

    M. J. Townsend

    2000-05-01

    Well ER-EC-6 was drilled for the U.S. Department of Energy, Nevada Operations Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in the spring of 1999 as part of the DOE's hydrogeologic investigation well program in the Western Pahute Mesa - Oasis Valley region just west of the Nevada Test Site. A 66-centimeter surface hole was drilled and cased off to the depth of 485.1 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 1,524.0 meters. A preliminary composite, static, water level was measured at the depth of approximately 434.6 meters prior to installation of the completion string. One completion string with four isolated, slotted intervals was installed in the well. Detailed lithologic descriptions with preliminary stratigraphic assignments are included in the report. These are based on composite drill cuttings collected every 3 meters and 33 sidewall samples taken at various depths below 504.4 meters, supplemented by geophysical log data. Detailed chemical and mineralogical studies of rock samples are in progress. The well penetrated Tertiary-age lava and tuff of the Timber Mountain Group, the Paintbrush Group, the Calico Hills Formation, and the Volcanics of Quartz Mountain. Intense hydrothermal alteration was observed below the depth of 640 m. The preliminary geologic interpretation indicates that this site may be located on a buried structural ridge that separates the Silent Canyon and Timber Mountain caldera complexes.

  10. Completion Report for Well ER-EC-8

    SciTech Connect (OSTI)

    Bechtel Nevada

    2004-10-01

    Well ER-EC-8 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in the summer of 1999 as part of the U.S. Department of Energy's hydrogeologic investigation program in the Western Pahute Mesa - Oasis Valley region just west of the Nevada Test Site. A 44.5-centimeter surface hole was drilled and cased off to a depth of 129.8 meters below the surface. The hole diameter was then decreased to 31.1 centimeters for drilling to a total depth of 609.6 meters. One completion string with three isolated slotted intervals was installed in the well. A preliminary composite, static water level was measured at the depth of 98.4 meters, 24 days after installation of the completion string. Detailed lithologic descriptions with stratigraphic assignments are included in the report. These are based on evaluation of composite drill cuttings collected every 3 meters, and 20 sidewall samples taken at various depths below 157.9 meters, supplemented by geophysical log data and results of detailed chemical and mineralogical studies of rock samples. Drilling began in Tertiary-age tuff of the Thirsty Canyon Group, and penetrated tuffs of the Beatty Wash Formation, tuff of Buttonhook Wash, and the upper portion of the Ammonia Tanks Tuff. The geologic interpretation of data from this well helps define the location of the western margin of the Timber Mountain caldera complex in the southwestern Nevada volcanic field. Geologic and hydrologic data from the well will aid in development of models to predict groundwater flow and contaminant migration within and near the Nevada Test Site.

  11. Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic

    Office of Scientific and Technical Information (OSTI)

    Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio (Technical Report) | SciTech Connect Technical Report: Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio Citation Details In-Document Search Title: Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and

  12. Award DE-FG02-04ER52655 Final Technical Report: Interior Point Algorithms

    Office of Scientific and Technical Information (OSTI)

    for Optimization Problems (Technical Report) | SciTech Connect Award DE-FG02-04ER52655 Final Technical Report: Interior Point Algorithms for Optimization Problems Citation Details In-Document Search Title: Award DE-FG02-04ER52655 Final Technical Report: Interior Point Algorithms for Optimization Problems Over the period of this award we developed an algorithmic framework for constraint reduction in linear programming (LP) and convex quadratic programming (QP), proved convergence of our

  13. DYNAMICAL STABILITY AND QUANTUM CHAOS OF IONS IN A LINEAR TRAP (1999002ER).

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    (Technical Report) | SciTech Connect Technical Report: DYNAMICAL STABILITY AND QUANTUM CHAOS OF IONS IN A LINEAR TRAP (1999002ER). Citation Details In-Document Search Title: DYNAMICAL STABILITY AND QUANTUM CHAOS OF IONS IN A LINEAR TRAP (1999002ER). The realization of a paradigm chaotic system, namely the harmonically driven oscillator, in the quantum domain using cold trapped ions driven by lasers is theoretically investigated. The simplest characteristics of regular and chaotic dynamics

  14. Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio (Technical Report) | SciTech Connect Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio Citation Details In-Document Search Title: Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of

  15. Self-Assembled ErSb Nanostructures with Optical Applications in Infrared

    Office of Scientific and Technical Information (OSTI)

    and Terahertz (Journal Article) | SciTech Connect Self-Assembled ErSb Nanostructures with Optical Applications in Infrared and Terahertz Citation Details In-Document Search Title: Self-Assembled ErSb Nanostructures with Optical Applications in Infrared and Terahertz Authors: Lu, Hong ; Ouellette, Daniel G. ; Preu, Sascha ; Watts, Justin D. ; Zaks, Benjamin ; Burke, Peter G. ; Sherwin, Mark S. ; Gossard, Arthur C. Publication Date: 2014-03-12 OSTI Identifier: 1160953 DOE Contract Number:

  16. IMG ER: A System for Microbial Genome Annotation Expert Review and Curation

    SciTech Connect (OSTI)

    Markowitz, Victor M.; Mavromatis, Konstantinos; Ivanova, Natalia N.; Chen, I-Min A.; Chu, Ken; Kyrpides, Nikos C.

    2009-05-25

    A rapidly increasing number of microbial genomes are sequenced by organizations worldwide and are eventually included into various public genome data resources. The quality of the annotations depends largely on the original dataset providers, with erroneous or incomplete annotations often carried over into the public resources and difficult to correct. We have developed an Expert Review (ER) version of the Integrated Microbial Genomes (IMG) system, with the goal of supporting systematic and efficient revision of microbial genome annotations. IMG ER provides tools for the review and curation of annotations of both new and publicly available microbial genomes within IMG's rich integrated genome framework. New genome datasets are included into IMG ER prior to their public release either with their native annotations or with annotations generated by IMG ER's annotation pipeline. IMG ER tools allow addressing annotation problems detected with IMG's comparative analysis tools, such as genes missed by gene prediction pipelines or genes without an associated function. Over the past year, IMG ER was used for improving the annotations of about 150 microbial genomes.

  17. Pahute Mesa Well Development and Testing Analyses for Wells ER-20-8 and ER-20-4, Nevada National Security Site, Nye County, Nevada, Revision 0

    SciTech Connect (OSTI)

    Greg Ruskauff and Sam Marutzky

    2012-09-01

    Wells ER-20-4 and ER-20-8 were drilled during fiscal year (FY) 2009 and FY 2010 (NNSA/NSO, 2011a and b). The closest underground nuclear test detonations to the area of investigation are TYBO (U-20y), BELMONT (U-20as), MOLBO (U-20ag), BENHAM (U-20c), and HOYA (U-20 be) (Figure 1-1). The TYBO, MOLBO, and BENHAM detonations had working points located below the regional water table. The BELMONT and HOYA detonation working points were located just above the water table, and the cavity for these detonations are calculated to extend below the water table (Pawloski et al., 2002). The broad purpose of Wells ER-20-4 and ER-20-8 is to determine the extent of radionuclide-contaminated groundwater, the geologic formations, groundwater geochemistry as an indicator of age and origin, and the water-bearing properties and hydraulic conditions that influence radionuclide migration. Well development and testing is performed to determine the hydraulic properties at the well and between other wells, and to obtain groundwater samples at the well that are representative of the formation at the well. The area location, wells, underground nuclear detonations, and other features are shown in Figure 1-1. Hydrostratigraphic cross sections A-A, B-B, C-C, and D-D are shown in Figures 1-2 through 1-5, respectively.

  18. Final Technical Report for DOE Grant DE-FG02-91ER20038 (Technical Report) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Technical Report: Final Technical Report for DOE Grant DE-FG02-91ER20038 Citation Details In-Document Search Title: Final Technical Report for DOE Grant DE-FG02-91ER20038 The existence of species within the plant genus Flaveria with differing leaf cell arrangements and photosynthetic schemes (C3, C4, C3-C4) enabled us to identify genetic elements (promoters, 3'UTRs) that are responsible for the regulation of pre-existing metabolic genes in the pattern required for the

  19. DYNAMICAL STABILITY AND QUANTUM CHAOS OF IONS IN A LINEAR TRAP (1999002ER).

    Office of Scientific and Technical Information (OSTI)

    (Technical Report) | SciTech Connect Technical Report: DYNAMICAL STABILITY AND QUANTUM CHAOS OF IONS IN A LINEAR TRAP (1999002ER). Citation Details In-Document Search Title: DYNAMICAL STABILITY AND QUANTUM CHAOS OF IONS IN A LINEAR TRAP (1999002ER). × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and Technical Information (OSTI) and is provided as a public service. Visit OSTI to utilize additional

  20. Final Technical Report of Project DE-FG02-96ER14647 (Technical Report) |

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Final Technical Report of Project DE-FG02-96ER14647 Citation Details In-Document Search Title: Final Technical Report of Project DE-FG02-96ER14647 This is the final technical report of work completed under DOE support over the period Sept. 1, 1996 until May 31, 2015. The title of the project was "Ion/Excited Atom Collision Studies with a Rydberg Target and a CO2 Laser" from 9/1/96 to 10/31/06, and "Properties of Actinide Ions from Measurements of Rydberg Ion

  1. The photoluminescence properties of Er{sup 3+}-doped ZrO{sub 2} nanotube arrays prepared by anodization

    SciTech Connect (OSTI)

    Wang, Xixin; Zhao, Jianling; Du, Peng; Guo, Limin; Xu, Xuewen; Tang, Chengchun

    2012-11-15

    Graphical abstract: Display Omitted Highlights: ? Er{sup 3+}-doped ZrO{sub 2} nanotube arrays were prepared by anodization of ZrEr alloy. ? Small tetragonal zirconia crystallites are tended to be formed due to the doping of Er{sup 3+}. ? Under excitation at 317 nm, the ZrO{sub 2} nantube arrays have strongest photoluminescence intensity. -- Abstract: Er{sup 3+}-doped ZrO{sub 2} nanotube arrays were prepared by anodization of ZrEr alloy which was obtained by melting zirconium with 1.0 wt% erbium. The morphology, structure and photoluminescence properties were studied through scanning electron microscope, transmission electron microscope, X-ray diffraction and photoluminescence analyzer. X-ray diffraction results indicate that doping of Er{sup 3+} affects the crystal structure and grain size obviously and the Er{sup 3+}-doped samples tend to form small tetragonal grains. Photoluminescence analyses show that when Er{sup 3+}-doped zirconia nanotube arrays are excited at 317 nm, there are two strong photoluminescence emission peaks at 373 nm and 415 nm. When the excitation wavelength is 257 nm, a photoluminescence emission peak appears at 363 nm. Under same measurement conditions, emission peaks of the undoped ZrO{sub 2} nanotube arrays are very weak.

  2. Electrically active Er doping in InAs, In{sub 0.53}Ga{sub 0.47...

    Office of Scientific and Technical Information (OSTI)

    Electrically active Er doping in InAs, Insub 0.53Gasub 0.47As, and GaAs Citation Details In-Document Search Title: Electrically active Er doping in InAs, Insub 0.53Gasub ...

  3. Final Report on Research Conducted under Grant DE-FG02-98ER14857

    SciTech Connect (OSTI)

    Carpenter, Barry,K.; Davis, H., Floyd

    2008-01-28

    Work in the Carpenter laboratory under the aegis of grant DE-FG02-98ER14857 concerned the formation, properties, and reactions of organic free radicals known or believed to be important in hydrocarbon combustion. Both computational and experimental methods were employed in these studies.

  4. FOEU-iERLY UTILIZED SITES REKEDIAL ACTION PROG%AM ELIMINATION REPORT

    Office of Legacy Management (LM)

    y ((-' q ' c - ,' .* FOEU-iERLY UTILIZED SITES REKEDIAL ACTION PROG%AM ELIMINATION REPORT FORMERERATOOLAND ENGINEERING COMPANY 4555 UEST ADDISON STREET CHICAGO, ILLINOIS NOVEMBER 14, 1989 Department of Energy Office of Nuclear Energy Office of Remedial Action and Waste Technology Facility and Site Decommissioning . . CONTENTS Page INTRODU~ION......................... 1 BAcI(GROuND.......................... 1 Site Function. ...................... 1 Site Description ..................... 2

  5. Fluoride-elicited developmental testicular toxicity in rats: Roles of endoplasmic reticulum stress and inflammatory response

    SciTech Connect (OSTI)

    Zhang, Shun; Jiang, Chunyang; Liu, Hongliang; Guan, Zhizhong; Zeng, Qiang; Zhang, Cheng; Lei, Rongrong; Xia, Tao; Gao, Hui; Yang, Lu; Chen, Yihu; Wu, Xue; Zhang, Xiaofei; Cui, Yushan; Yu, Linyu; Wang, Zhenglun; Wang, Aiguo

    2013-09-01

    Long-term excessive fluoride intake is known to be toxic and can damage a variety of organs and tissues in the human body. However, the molecular mechanisms underlying fluoride-induced male reproductive toxicity are not well understood. In this study, we used a rat model to simulate the situations of human exposure and aimed to evaluate the roles of endoplasmic reticulum (ER) stress and inflammatory response in fluoride-induced testicular injury. SpragueDawley rats were administered with sodium fluoride (NaF) at 25, 50 and 100 mg/L via drinking water from pre-pregnancy to gestation, birth and finally to post-puberty. And then the testes of male offspring were studied at 8 weeks of age. Our results demonstrated that fluoride treatment increased MDA accumulation, decreased SOD activity, and enhanced germ cell apoptosis. In addition, fluoride elevated mRNA and protein levels of glucose-regulated protein 78 (GRP78), inositol requiring ER-to-nucleus signal kinase 1 (IRE1), and C/EBP homologous protein (CHOP), indicating activation of ER stress signaling. Furthermore, fluoride also induced testicular inflammation, as manifested by gene up-regulation of tumor necrosis factor-? (TNF-?), interleukin-1? (IL-1?), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in a nuclear factor-?B (NF-?B)-dependent manner. These were associated with marked histopathological lesions including injury of spermatogonia, decrease of spermatocytes and absence of elongated spermatids, as well as severe ultrastructural abnormalities in testes. Taken together, our results provide compelling evidence that ER stress and inflammation would be novel and significant mechanisms responsible for fluoride-induced disturbance of spermatogenesis and germ cell loss in addition to oxidative stress. - Highlights: We used a rat model to simulate the situations of human fluoride (F) exposure. Developmental F exposure induces testicular damage related with oxidative stress. Endoplasmic reticulum stress is involved in testis disorder and germ cell apoptosis. Inflammatory response is implicated in impaired spermatogenesis and germ cell loss.

  6. DOE-ER-STD-6001-92; Implementation Guide for Quality Assurance Programs for Basic and applied Research

    Office of Environmental Management (EM)

    D9E-ER-STD--6001 -92 DE92 016352 DOE STANDARD IMPLEMENTATION GUIDE FOR QUALITY ASSURANCE PROGRAMS FOR BASIC AND APPLIED RESEARCH U.S. Department of Energy Washington, D.C. 20585 AREA-QCIC -~ This report has been reproduced directly from the best available copy. Available to the public from the National Technical Information Service, U.S. Department of Commerce, 5285 Port Royal Rd., Springfield, VA 22161. DOE-ER-STD-6001-92 ACKNOWLEDGEMENT The Office of Energy Research (ER) wishes to acknowledge

  7. Photonic crystal light emitting diode based on Er and Si nanoclusters co-doped slot waveguide

    SciTech Connect (OSTI)

    Lo Savio, R.; Galli, M.; Liscidini, M.; Andreani, L. C.; Franz, G.; Iacona, F.; Miritello, M.; Irrera, A.; Sanfilippo, D.; Piana, A.; Priolo, F.

    2014-03-24

    We report on the design, fabrication, and electro-optical characterization of a light emitting device operating at 1.54??m, whose active layer consists of silicon oxide containing Er-doped Si nanoclusters. A photonic crystal (PhC) is fabricated on the top-electrode to enhance the light extraction in the vertical direction, and thus the external efficiency of the device. This occurs if a photonic mode of the PhC slab is resonant with the Er emission energy, as confirmed by theoretical calculations and experimental analyses. We measure an increase of the extraction efficiency by a factor of 3 with a high directionality of light emission in a narrow vertical cone. External quantum efficiency and power efficiency are among the highest reported for this kind of material. These results are important for the realization of CMOS-compatible efficient light emitters at telecom wavelengths.

  8. Cw laser action of Er/sup 3 +/ in double sensitized fluoroaluminate glass at room temperature

    SciTech Connect (OSTI)

    Heumann, E.; Ledig, M.; Ehrt, D.; Seeber, W.; Duczynski, E.W.; Heide, H.v.; Huber, G.

    1988-01-25

    cw lasing at 1.6 ..mu..m was obtained for the first time in Cr, Yb, Er:fluoroaluminate glass. Double step pumping via Cr/sup 3 +/ and Yb/sup 3 +/ with a krypton laser yields a threshold pump power of 80 mW. Efficient lasing can be expected using glass samples of optimized dopant concentration and improved optical quality.

  9. 10/8/2014Final Report for Award # ER64999Page 1

    Office of Scientific and Technical Information (OSTI)

    0/8/2014Final Report for Award # ER64999Page 1 Title of Project: Methanogenic archaea and the global carbon cycle: a systems biology approach to the study of Methanosarcina species Institution: University of Illinois Date Received: 6-14-2010 Principal Investigator: William W. Metcalf Co-PIs: Nathan Price, Rachel Whitaker, Ping Ma Project Results: The long-term goal of this multi-investigator project was the creation of integrated, multiscale models that accurately and quantitatively predict the

  10. Final Report Department of Energy Grant FG02-06ER64189

    Office of Scientific and Technical Information (OSTI)

    26 Final Report Department of Energy Grant FG02-06ER64189 Colloid-Facilitated Transport of Cations in an Unsaturated Fractured Soil under Transient Conditions by Joseph N. Ryan1, Sanjay Mohanty1, and James E. Saiers2 1 Department of Civil, Environmental, and Architectural Engineering University of Colorado Boulder UCB 428 Boulder, Colorado 80309 2 School of Forestry and Environmental Studies Yale University 195 Prospect Street New Haven, Connecticut 06511 January 31, 2015 Abstract Rainfall

  11. Microsoft Word - Final Technical Report DE-FG02-07ER15891 Tien PSU REVISED

    Office of Scientific and Technical Information (OSTI)

    ER15891 Principal Investigator: Dr. Ming Tien Project Title: Modification of Lignin by Protein Cross-linking to Facilitate Production of Biofuels From Poplar The limited supply of fossil fuels and the associated environmental issues associated with their utilization has resulted in much effort put forth to promote renewable resources of energy. Switching to renewable fuels for energy will allow us to become carbon neutral by recycling carbon from plants and reduce carbon from dioxide emissions,

  12. Microsoft Word - FinalReport DE-FG02-08ER46494

    Office of Scientific and Technical Information (OSTI)

    STATISTICAL MECHANICS MODELING OF MESOSCALE DEFORMATION IN METALS DE-FG02-08ER46494 Period of Performance: June 1, 2008 - May 31, 2012 Submitted to Mechanical Behavior and Radiation Effects Program Division of Materials Science and Engineering/Office of Basic Energy Sciences U.S. Department of Energy Program Manager: Dr. John S. Vetrano Principal Investigator Anter El-Azab, Professor Current Affiliation and Contact Information: School of Nuclear Engineering and School of Materials Engineering

  13. Magnetic properties of Er1-xDyxAl2 (0 ≤ x ≤ 1) compounds in low applied

    Office of Scientific and Technical Information (OSTI)

    fields (Conference) | SciTech Connect properties of Er1-xDyxAl2 (0 ≤ x ≤ 1) compounds in low applied fields Citation Details In-Document Search Title: Magnetic properties of Er1-xDyxAl2 (0 ≤ x ≤ 1) compounds in low applied fields Low field magnetic properties of the Laves phase RAl{sub 2} compounds (where R is a mixture of Er and Dy, i.e., Er{sub 1-x}Dy{sub x} and x = 0, 0.1, 0.14, 0.18, 0.25, 0.33, 0.5, and 1) have been studied. These compounds order ferromagnetically, and the

  14. Analysis of Well ER-EC-6 Testing, Western Pahute Mesa - Oasis Valley FY 2000 Testing Program

    SciTech Connect (OSTI)

    2002-09-30

    This report documents the analysis of the data collected for Well ER-EC-6 during the Western Pahute Mesa-Oasis Valley (WPM-OV) well development and testing program that was conducted during fiscal year (FY) 2000. The data collection for that program is documented in Appendix A, Western Pahute Mesa-Oasis Valley, Well ER-EC-6 Data Report for Development and Hydraulic Testing.

  15. Final Technical Report: DE-FG02-08ER41562

    Office of Scientific and Technical Information (OSTI)

    Report: DE-FG02-08ER41562 (2008-2010) Discovering the Nature of Dark Energy: Towards Better Distances from Type Ia Supernovae PI: Prof. Saurabh W. Jha Rutgers, the State University of New Jersey 1 Journal Articles Produced This award supported PI Jha and his postdoc Benjamin Dilday, and resulted in the following 16 refereed journal publications: 1. "Luminosity Indicators in the Ultraviolet Spectra of Type Ia Supernovae, " Foley, R. J., A. V. Filippenko, and S. W. Jha, 2008, The

  16. Absence of exchange interaction between localized magnetic moments and conduction-electrons in diluted Er{sup 3+} gold-nanoparticles

    SciTech Connect (OSTI)

    Lesseux, G. G. Urbano, R. R.; Iwamoto, W.; Garca-Flores, A. F.; Rettori, C.

    2014-05-07

    The Electron Spin Resonance (ESR) of diluted Er{sup 3+} magnetic ions in Au nanoparticles (NPs) is reported. The NPs were synthesized by reducing chloro triphenyl-phosphine gold(I) and erbium(III) trifluoroacetate. The Er{sup 3+} g-value along with the observed hyperfine splitting indicate that the Er{sup 3+} impurities are in a local cubic symmetry. Furthermore, the Er{sup 3+} ESR spectra show that the exchange interaction between the 4f and the conduction electrons (ce) is absent or negligible in Au{sub 1x}Er{sub x} NPs, in contrast to the ESR results in bulk Au{sub 1x}Er{sub x}. Therefore, the nature of this interaction needs to be reexamined at the nano scale range.

  17. Electrical Properties of Er-doped In0.53Ga0.47As

    SciTech Connect (OSTI)

    Burke, Peter G.; Lu, Hong; Rudawski, Nicholas G.; Stemmer, Susanne; Gossard, Arthur C.; Bahk, Je-Hyeong; Bowers, John E.

    2011-03-03

    The electrical properties of In0.53Ga0.47As As thin films Er-doped to concentrations of 1.51017 7.21020 cm-3 grown by molecular beam epitaxy at 490 C on (001) InP substrates were studied. Electrical conductivity, carrier density, and carrier mobility as a function of Er doping were measured by Hall effect at temperatures of 20750 K. Additionally, high-angle annular dark-field scanning transmission electron microscopy and infrared absorption spectroscopy confirmed the presence of epitaxially embedded ErAs nanoparticles at Er concentrations ?81019 cm-3. The observed electrical properties are discussed in terms of the dependence of ErAs nanoparticle formation with Er doping.

  18. Er{sup 3+}/Yb{sup 3+}co-doped bismuth molybdate nanosheets upconversion photocatalyst with enhanced photocatalytic activity

    SciTech Connect (OSTI)

    Adhikari, Rajesh; Gyawali, Gobinda; Cho, Sung Hun; Narro-Garca, R.; Sekino, Tohru; Lee, Soo Wohn

    2014-01-15

    In this paper, we report the microwave hydrothermal synthesis of Er{sup 3+}/Yb{sup 3+} co-doped Bi{sub 2}MoO{sub 6} upconversion photocatalyst. Crystal structure, morphology, elemental composition, optical properties and BET surface area were analyzed in detail. Infrared to visible upconversion luminescence at 532 nm and 546 nm of the co-doped samples was investigated under excitation at 980 nm. The results revealed that the co-doping of Er{sup 3+}/Yb{sup 3+} into Bi{sub 2}MoO{sub 6} exhibited enhanced photocatalytic activity for the decomposition of rhodamine B under simulated solar light irradiation. Enhanced photocatalytic activity can be attributed to the energy transfer between Er{sup 3+}/Yb{sup 3+} and Bi{sub 2}MoO{sub 6} via infrared to visible upconversion from Er{sup 3+}/Yb{sup 3+} ion and higher surface area of the Bi{sub 2}MoO{sub 6} nanosheets. Therefore, this synthetic approach may exhibit a better alternative to fabricate upconversion photocatalyst for integral solar light absorption. - Graphical abstract: Schematic illustration of the upconversion photocatalysis. Display Omitted - Highlights: Er{sup 3+}/Yb{sup 3+} co-doped Bi{sub 2}MoO{sub 6} upconversion photocatalyst is successfully synthesized. We obtained the nanosheets having high surface area. Upconversion of IR to visible light was confirmed. Upconversion phenomena can be utilized for effective photocatalysis.

  19. Development of bubble microstructure in ErT2 films during aging

    SciTech Connect (OSTI)

    Bond, Gillian; Snow, Clark

    2010-01-01

    Helium bubbles form in metal tritide films as tritium decays into 3He, influencing mechanical properties and long-term film stability. The bubble nucleation and growth mechanisms comprise an active research area, but there has been only one previous systematic experimental study of helium bubble growth in metal tritides, on zirconium tritides. There have been no such studies on tritides such as ErT2 that form plate-like bubbles and lack a secondary bubble population on a network of line dislocations, and yet such a study is needed to inform the modeling of helium-bubble microstructure development in a broader range of metal tritides. Transmission electron microscopy (TEM) has been used to study the growth and evolution of helium bubbles in ErT2 films over a four-year period. The results have been used to test the present models of helium bubble nucleation and growth in metal tritides, particularly those forming plate-like bubbles. The results support the models of Trinkaus and Cowgill. The observations of non-uniform bubble thicknesses and the pattern of grain-boundary bubble formation, however, indicate that these models could be strengthened by closer attention to details of interfacial energy. It is strongly recommended that efforts be made (either experimentally or by calculation) to determine anisotropy of tritide/helium interfacial energy, both for clean, stoichiometric interfaces, and also allowing for such factors as non-stoichiometry and segregation.

  20. The role of Nb in intensity increase of Er ion upconversion luminescence in zirconia

    SciTech Connect (OSTI)

    Smits, K. Sarakovskis, A.; Grigorjeva, L.; Millers, D.; Grabis, J.

    2014-06-07

    It is found that Nb co-doping increases the luminescence and upconversion luminescence intensity in rare earth doped zirconia. Er and Yb-doped nanocrystalline samples with or without Nb co-doping were prepared by sol-gel method and thermally annealed to check for the impact of phase transition on luminescence properties. Phase composition and grain sizes were examined by X-ray diffraction; the morphology was checked by scanning- and high-resolution transmission electron microscopes. Both steady-state and time-resolved luminescence were studied. Comparison of samples with different oxygen vacancy concentrations and different Nb concentrations confirmed the known assumption that oxygen vacancies are the main agents for tetragonal or cubic phase stabilization. The oxygen vacancies quench the upconversion luminescence; however, they also prevent agglomeration of rare-earth ions and/or displacement of rare-earth ions to grain surfaces. It is found that co-doping with Nb ions significantly (>20 times) increases upconversion luminescence intensity. Hence, ZrO{sub 2}:Er:Yb:Nb nanocrystals may show promise for upconversion applications.

  1. Final Technical Report DE-FG02-08ER41540. Establishing the Transport Properties of QCD With Heavy Ion Reactions

    SciTech Connect (OSTI)

    Teaney, Derek

    2015-03-17

    We review the results of the DE-FG02-08ER41540, "Establishing the transport properties of QCD with heavy ion reactions"

  2. Evidence of energy transfer in an aluminosilicate glass codoped with Si nanoaggregates and Er{sup 3+} ions

    SciTech Connect (OSTI)

    Enrichi, F.; Mattei, G.; Sada, C.; Trave, E.; Pacifici, D.; Franzo, G.; Priolo, F.; Iacona, F.; Prassas, M.; Falconieri, M.; Borsella, E.

    2004-10-01

    The enhancement of the Er{sup 3+} ions' photoluminescence (PL) emission at 1.54 {mu}m in a Si and Er coimplanted aluminosilicate glass is investigated in detail. A postimplantation thermal treatment has been performed to recover the damage induced by the implantation process and to promote Si aggregation. It will be shown that 1 h treatment in N{sub 2} atmosphere is not sufficient to induce Si precipitation for temperatures up to 500 deg. C. Nevertheless, the most intense Er{sup 3+} PL emission at 1.54 {mu}m is achieved after a thermal treatment at 400 deg. C. Such emission has been investigated by pumping in and out of resonance, showing a very efficient energy transfer process in the whole excitation wavelength range (360-515 nm). These results suggest that good energy transfer mediators could be small Si aggregates and not only crystalline clusters. For the best performing sample, the effective Er excitation cross section has been measured to be higher than 10{sup -17} cm{sup 2} at 379 and 390 nm and about 2x10{sup -16} cm{sup 2} at 476 nm, that is, several orders of magnitude higher than the Er direct absorption cross section (of the order of 10{sup -21} cm{sup 2} in this glass). Moreover the coefficient of cooperative upconversion has been evaluated to be 2.7x10{sup -18} cm{sup 3} s{sup -1}. The structural and optical properties of this material are discussed and compared to those found for Si and Er codoped silica.

  3. Controlling n-Type Carrier Density from Er Doping of InGaAs with MBE Growth

    Office of Scientific and Technical Information (OSTI)

    Temperature (Journal Article) | SciTech Connect Controlling n-Type Carrier Density from Er Doping of InGaAs with MBE Growth Temperature Citation Details In-Document Search Title: Controlling n-Type Carrier Density from Er Doping of InGaAs with MBE Growth Temperature Authors: Burke, Peter G. ; Buehl, Trevor E. ; Gilles, Pernot ; Lu, Hong ; Shakouri, Ali ; Palmstrom, C. J. ; Bowers, John E. ; Gossard, Arthur C. Publication Date: 2012-04-04 OSTI Identifier: 1066154 DOE Contract Number:

  4. Final Technical Report for DOE DE-FG02-05ER54831 "Laboratory Studies of

    Office of Scientific and Technical Information (OSTI)

    Dynamos." (Technical Report) | SciTech Connect Technical Report: Final Technical Report for DOE DE-FG02-05ER54831 "Laboratory Studies of Dynamos." Citation Details In-Document Search Title: Final Technical Report for DOE DE-FG02-05ER54831 "Laboratory Studies of Dynamos." Laboratory Studies of Dynamos: Executive Summary. The self-generation of magnetic #12;fields by astrophysical bodies like planets, stars, accretion disks, galaxies, and even galaxy clusters arises

  5. Final Report for Grant # DE-FG02-02ER46000 Simulations of Self-Assembly of

    Office of Scientific and Technical Information (OSTI)

    Tethered Nanoparticle Shape Amphiphiles (Technical Report) | SciTech Connect Final Report for Grant # DE-FG02-02ER46000 Simulations of Self-Assembly of Tethered Nanoparticle Shape Amphiphiles Citation Details In-Document Search Title: Final Report for Grant # DE-FG02-02ER46000 Simulations of Self-Assembly of Tethered Nanoparticle Shape Amphiphiles × You are accessing a document from the Department of Energy's (DOE) SciTech Connect. This site is a product of DOE's Office of Scientific and

  6. Closeout of the award DE-FG02-05ER46223. Trustees of the University of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Pennsylvania. Project title- "Modular Designed Protein Constructions for Solar Generated H2 From Water (Technical Report) | SciTech Connect Closeout of the award DE-FG02-05ER46223. Trustees of the University of Pennsylvania. Project title- "Modular Designed Protein Constructions for Solar Generated H2 From Water Citation Details In-Document Search Title: Closeout of the award DE-FG02-05ER46223. Trustees of the University of Pennsylvania. Project title- "Modular Designed

  7. Completion Report for Model Evaluation Well ER-11-2: Corrective Action Unit 98: Frenchman Flat

    SciTech Connect (OSTI)

    NSTec Underground Test Area and Boreholes Programs and Operations

    2013-01-22

    Model Evaluation Well ER-11-2 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of Nevada Environmental Management Operations at the Nevada National Security Site (formerly known as the Nevada Test Site). The well was drilled in August 2012 as part of a model evaluation program in the Frenchman Flat area of Nye County, Nevada. The primary purpose of the well was to provide detailed geologic, hydrogeologic, chemical, and radionuclide data that can be used to test and build confidence in the applicability of the Frenchman Flat Corrective Action Unit flow and transport models for their intended purpose. In particular, this well was designed to provide data to evaluate the uncertainty in model forecasts of contaminant migration from the upgradient underground nuclear test PIN STRIPE, conducted in borehole U-11b in 1966. Well ER-11-2 will provide information that can be used to refine the Phase II Frenchman Flat hydrostratigraphic framework model if necessary, as well as to support future groundwater flow and transport modeling. The main 31.1-centimeter (cm) hole was drilled to a total depth of 399.6 meters (m). A completion casing string was not set in Well ER-11-2. However, a piezometer string was installed in the 31.1-cm open hole. The piezometer is composed of 7.3-cm stainless-steel tubing hung on 6.0-cm carbon-steel tubing via a crossover sub. The piezometer string was landed at 394.5 m, for monitoring the lower tuff confining unit. Data collected during and shortly after hole construction include composite drill cuttings samples collected every 3.0 m, various geophysical logs, water quality (including tritium and other test-related radionuclides) measurements, and water level measurements. The well penetrated 42.7 m of Quaternary and Tertiary alluvium and 356.9 m of Tertiary volcanic rock. The water-level measured in the piezometer string on September 25, 2012, was 353.8 m below ground surface. No tritium above levels detectable by field methods were encountered in this hole. No well development or hydrologic testing was conducted in this well immediately after completion, and future well development, sampling, and hydrologic testing planned for this well will be limited due to the diameter of the piezometer string. The stratigraphy, general lithology, and the water level are as expected, but the section of geology encountered is higher than expected due to faulting. No tritium above the minimum detection limit of the field equipment was detected because the target aquifer (the Topopah Spring aquifer) at Well ER-11-2 is structurally higher than expected and thus unsaturated.

  8. Concentration effect of Er{sup 3+} ions on structural and spectroscopic properties of CdNb{sub 2}O{sub 6} phosphors

    SciTech Connect (OSTI)

    Ghafouri, Sanaz Aian; Erdem, Murat; Ekmeki, M. Kaan; Mergen, Ayhan; zen, Gnl

    2014-12-15

    Excitation and emission spectra of a visible room-temperature Er{sup 3+} ions luminescence from powders. - Highlights: This is the first report on spectroscopic properties of CdNb{sub 2}O{sub 6}:Er{sup 3+}. The crystalline sizes are affected as the concentration of Er{sup 3+} ions increased. Quenching of the luminescence was observed to be above 1.0 mol% Er{sup 3+}. - Abstract: This study is focused on the synthesis and characterization of CdNb{sub 2}O{sub 6} compounds doped with of Er{sup 3+} ions. Powders were synthesized by using the molten salt method and annealed at 900 C for 4 h. The synthesized particles were structurally characterized by using X-ray diffraction, scanning electron microscopy. A single phase of the CdNb{sub 2}O{sub 6} was determined and the size of the particles was found to be affected by the presence and the concentration of Er{sup 3+} ions. Luminescence properties of each sample were investigated by measuring accurately the emission and excitation spectra at room temperature in the wavelength range of 2001700 nm by exciting the Er{sup 3+} ions at 379 nm and 805 nm. Quenching of the luminescence in both visible and near infrared spectral regions was observed to be above 1.0 mol% Er{sup 3+} concentration.

  9. The structural and magnetic properties of Pr{sub 1−x}Er{sub x}Al{sub 2}

    SciTech Connect (OSTI)

    Pathak, Arjun K.; Gschneidner, K. A.; Pecharsky, V. K.

    2015-05-07

    We report on the effect of Er addition to PrAl{sub 2} on the lattice parameters, magnetic behavior, heat capacity, and magnetocaloric effect by using x-ray diffraction, magnetization, and heat capacity measurements. Unlike Pr{sub 0.6}Er{sub 0.4}Al{sub 2}, other alloys we studied in the pseudobinary (Pr{sub 1−x}Er{sub x})Al{sub 2} system do not exhibit a sharp peak in heat capacity with the application of magnetic field. Both the cubic lattice parameter and the Curie temperature decrease with increasing Er concentration. The nuclear specific heat coefficient decreases from 660 mJ K mol{sup −1} for x = 0.05 to a nearly negligible value for x = 0.95. The magnetic entropy and adiabatic temperature change varies from 2 to 4 J mol{sup −1} K{sup −1} and 2.5 to 5 K at ΔH = 20 kOe for x = 0.05 to 0.95, respectively. These values of the magnetocaloric effect are comparable to those of the other rare-earth dialuminides systems.

  10. The crystal structure of {pi}-ErBO{sub 3}: New single-crystal data for an old problem

    SciTech Connect (OSTI)

    Pitscheider, Almut [Institut fuer Allgemeine, Anorganische und Theoretische Chemie, Leopold-Franzens-Universitaet Innsbruck, Innrain 52a, A-6020 Innsbruck (Austria); Kaindl, Reinhard [Institut fuer Mineralogie und Petrographie, Leopold-Franzens-Universitaet Innsbruck, Innrain 52, A-6020 Innsbruck (Austria); Oeckler, Oliver [Department Chemie, Ludwig-Maximilians-Universitaet Muenchen, Butenandtstrasse 5-13, D-81377 Muenchen (Germany); Huppertz, Hubert, E-mail: Hubert.Huppertz@uibk.ac.a [Institut fuer Allgemeine, Anorganische und Theoretische Chemie, Leopold-Franzens-Universitaet Innsbruck, Innrain 52a, A-6020 Innsbruck (Austria)

    2011-01-15

    Single crystals of the orthoborate {pi}-ErBO{sub 3} were synthesized from Er{sub 2}O{sub 3} and B{sub 2}O{sub 3} under high-pressure/high-temperature conditions of 2 GPa and 800 {sup o}C in a Walker-type multianvil apparatus. The crystal structure was determined on the basis of single-crystal X-ray diffraction data, collected at room temperature. The title compound crystallizes in the monoclinic pseudowollastonite-type structure, space group C2/c, with the lattice parameters a=1128.4(2) pm, b=652.6(2) pm, c=954.0(2) pm, and {beta}=112.8(1){sup o} (R{sub 1}=0.0124 and wR{sub 2}=0.0404 for all data). -- graphical abstract: The first satisfying single-crystal structure determination of {pi}-ErBO{sub 3} sheds light on the extensively discussed structure of {pi}-orthoborates. The application of light pressure during the solid state synthesis yielded in high-quality crystals, due to pressure-induced crystallization. Research highlights: {yields} High-quality single crystals of {pi}-ErBO{sub 3} were prepared via high-pressure-induced crystallization. {yields} At least five different space groups for the rare-earth {pi}-orthoborates are reported. {yields} {pi}-ErBO{sub 3} is isotypic to the pseudowollastonite-type CaSiO{sub 3}. {yields} Remaining ambiguities regarding the structure of the rare-earth {pi}-orthoborates are resolved.

  11. Prenatal PCBs disrupt early neuroendocrine development of the rat hypothalamus

    SciTech Connect (OSTI)

    Dickerson, Sarah M.; Cunningham, Stephanie L. [Center for Molecular and Cellular Toxicology, Division of Pharmacology and Toxicology, University of Texas at Austin, Austin, TX 78712 (United States); Gore, Andrea C., E-mail: andrea.gore@mail.utexas.edu [Center for Molecular and Cellular Toxicology, Division of Pharmacology and Toxicology, University of Texas at Austin, Austin, TX 78712 (United States); Institute for Neuroscience, University of Texas at Austin, Austin, TX 78712 (United States); Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712 (United States)

    2011-04-01

    Neonatal exposure to endocrine disrupting chemicals (EDCs) such as polychlorinated biphenyls (PCBs) can interfere with hormone-sensitive developmental processes, including brain sexual differentiation. We hypothesized that disruption of these processes by gestational PCB exposure would be detectable as early as the day after birth (postnatal day (P) 1) through alterations in hypothalamic gene and protein expression. Pregnant Sprague-Dawley rats were injected twice, once each on gestational days 16 and 18, with one of the following: DMSO vehicle; the industrial PCB mixture Aroclor 1221 (A1221); a reconstituted mixture of the three most prevalent congeners found in humans, PCB138, PCB153, and PCB180; or estradiol benzoate (EB). On P1, litter composition, anogenital distance (AGD), and body weight were assessed. Pups were euthanized for immunohistochemistry of estrogen receptor {alpha} (ER{alpha}) or TUNEL labeling of apoptotic cells or quantitative PCR of 48 selected genes in the preoptic area (POA). We found that treatment with EB or A1221 had a sex-specific effect on developmental apoptosis in the neonatal anteroventral periventricular nucleus (AVPV), a sexually dimorphic hypothalamic region involved in the regulation of reproductive neuroendocrine function. In this region, exposed females had increased numbers of apoptotic nuclei, whereas there was no effect of treatment in males. For ER{alpha}, EB treatment increased immunoreactive cell numbers and density in the medial preoptic nucleus (MPN) of both males and females, while A1221 and the PCB mixture had no effect. PCR analysis of gene expression in the POA identified nine genes that were significantly altered by prenatal EDC exposure, in a manner that varied by sex and treatment. These genes included brain-derived neurotrophic factor, GABA{sub B} receptors-1 and -2, IGF-1, kisspeptin receptor, NMDA receptor subunits NR2b and NR2c, prodynorphin, and TGF{alpha}. Collectively, these results suggest that the disrupted sexual differentiation of the POA by prenatal EDC exposures is already evident as early as the day after birth, effects that may change the trajectory of postnatal development and compromise adult reproductive function.

  12. The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: Involvement of interleukin-2 system

    SciTech Connect (OSTI)

    Cervia, Davide; Department of Biomedical and Clinical Sciences, Luigi Sacco University Hospital, University of Milan, Milano ; Catalani, Elisabetta; Belardinelli, Maria Cristina; Perrotta, Cristiana; Picchietti, Simona; Alimenti, Claudio; Casini, Giovanni; Fausto, Anna Maria; Vallesi, Adriana

    2013-02-01

    Water-soluble protein signals (pheromones) of the ciliate Euplotes have been supposed to be functional precursors of growth factors and cytokines that regulate cellcell interaction in multi-cellular eukaryotes. This work provides evidence that native preparations of the Euplotes raikovi pheromone Er-1 (a helical protein of 40 amino acids) specifically increases viability, DNA synthesis, proliferation, and the production of interferon-?, tumor necrosis factor-?, interleukin (IL)-1?, IL-2, and IL-13 in human Jurkat T-cells. Also, Er-1 significantly decreases the mRNA levels of the ? and ? subunits of IL-2 receptor (IL-2R), while the mRNA levels of the ? subunit appeared to be not affected. Jurkat T-cell treatments with Er-1 induced the down-regulation of the IL-2R? subunit by a reversible and time-dependent endocytosis, and increased the levels of phosphorylation of the extracellular signal-regulated kinases (ERK). The cell-type specificity of these effects was supported by the finding that Er-1, although unable to directly influence the growth of human glioma U-373 cells, induced Jurkat cells to synthesize and release factors that, in turn, inhibited the U-373 cell proliferation. Overall, these findings imply that Er-1 coupling to IL-2R and ERK immuno-enhances T-cell activity, and that this effect likely translates to an inhibition of glioma cell growth. -- Highlights: ? Euplotes pheromone Er-1 increases the growth of human Jurkat T-cells. ? Er-1 increases the T-cell production of specific cytokines. ? Er-1 activates interleukin-2 receptor and extracellular signal-regulated kinases. ? The immuno-enhancing effect of Er-1 on Jurkat cells translates to an inhibition of human glioma cell growth.

  13. Helium release and microstructural changes in Er(D,T)2-x3Hex films).

    SciTech Connect (OSTI)

    Gelles, D. S.; Browning, James Frederick; Snow, Clark Sheldon; Banks, James Clifford; Mangan, Michael A.; Rodriguez, Mark Andrew; Brewer, Luke N.; Kotula, Paul Gabriel

    2007-12-01

    Er(D,T){sub 2-x} {sup 3}He{sub x}, erbium di-tritide, films of thicknesses 500 nm, 400 nm, 300 nm, 200 nm, and 100 nm were grown and analyzed by Transmission Electron Microscopy, X-Ray Diffraction, and Ion Beam Analysis to determine variations in film microstructure as a function of film thickness and age, due to the time-dependent build-up of {sup 3}He in the film from the radioactive decay of tritium. Several interesting features were observed: One, the amount of helium released as a function of film thickness is relatively constant. This suggests that the helium is being released only from the near surface region and that the helium is not diffusing to the surface from the bulk of the film. Two, lenticular helium bubbles are observed as a result of the radioactive decay of tritium into {sup 3}He. These bubbles grow along the [111] crystallographic direction. Three, a helium bubble free zone, or 'denuded zone' is observed near the surface. The size of this region is independent of film thickness. Four, an analysis of secondary diffraction spots in the Transmission Electron Microscopy study indicate that small erbium oxide precipitates, 5-10 nm in size, exist throughout the film. Further, all of the films had large erbium oxide inclusions, in many cases these inclusions span the depth of the film.

  14. Final Technical Report for Award DE-FG02-98ER41080

    SciTech Connect (OSTI)

    Chen, Alan

    2014-11-14

    The prime motivation of the project at McMaster University was to carry out the critical evaluation and compilation of Nuclear Structure and Decay data, and of nuclear astrophysics data with continued participation in the United States Nuclear Data Program (US-NDP). A large body of evaluated and compiled structure data were supplied for databases such as ENSDF, XUNDL, NSR, etc. residing on webpage of National Nuclear Data Center of the Brookhaven National Laboratory, Upton, New York, USA. Thermonuclear reaction rates of importance to stellar explosions, such as novae, x-ray bursts and supernovae, were evaluated as well. This effort was closely coupled to our ongoing experimental effort, which took advantage of radioactive ion beam and stable beam facilities worldwide to study these key reaction rates. This report contains brief descriptions of the various activities together with references to all the publications in peer-reviewed journals which were the result of work carried out with the award DE-FG02-98-ER41080, during 1998-2013.

  15. THERMODYNAMIC AND KINETIC MODELING OF ADVANCED NUCLEAR FUELS - FINAL LDRD-ER REPORT

    SciTech Connect (OSTI)

    Turchi, P

    2011-11-28

    This project enhanced our theoretical capabilities geared towards establishing the basic science of a high-throughput protocol for the development of advanced nuclear fuel that should couple modern computational materials modeling and simulation tools, fabrication and characterization capabilities, and targeted high throughput performance testing experiments. The successful conclusion of this ER project allowed us to upgrade state-of-the-art modeling codes, and apply these modeling tools to ab initio energetics and thermodynamic assessments of phase diagrams of various mixtures of actinide alloys, propose a tool for optimizing composition of complex alloys for specific properties, predict diffusion behavior in diffusion couples made of actinide and transition metals, include one new equation in the LLNL phase-field AMPE code, and predict microstructure evolution during alloy coring. In FY11, despite limited funding, the team also initiated an experimental activity, with collaboration from Texas A&M University by preparing samples of nuclear fuels in bulk forms and for diffusion couple studies and metallic matrices, and performing preliminary characterization.

  16. Analysis of Well ER-6-2 Testing, Yucca Flat FY 2004 Testing Program, Nevada Test Site, Nye County, Nevada, Rev. No.: 0

    SciTech Connect (OSTI)

    Greg Ruskauff

    2005-07-01

    This report documents the analysis of data collected for Well ER-6-2 during fiscal year (FY) 2004 Yucca Flat well development and testing program (herein referred to as the ''testing program''). Participants in Well ER-6-2 field development and hydraulic testing activities were: Stoller-Navarro Joint Venture (SNJV), Bechtel Nevada (BN), Desert Research Institute (DRI), Los Alamos National Laboratory (LANL), Lawrence Livermore National Laboratory (LLNL), U.S. Geological Survey (USGS), and the University of Nevada, Las Vegas-Harry Reid Center (UNLV-HRC). The analyses of data collected from the Well ER-6-2 testing program were performed by the SNJV.

  17. Spatially resolved penetration depth measurements and vortex manipulation in the ferromagnetic superconductor ErNi2B2C

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wulferding, Dirk; Yang, Ilkyu; Yang, Jinho; Lee, Minkyung; Choi, Hee Cheul; Bud'ko, Sergey L.; Canfield, Paul C.; Yeom, Han Woong; Kim, Jeehoon

    2015-07-31

    We present a local probe study of the magnetic superconductor ErNi2B2C, using magnetic force microscopy at sub-Kelvin temperatures. ErNi2B2C is an ideal system to explore the effects of concomitant superconductivity and ferromagnetism. At 500 mK, far below the transition to a weakly ferromagnetic state, we directly observe a structured magnetic background on the micrometer scale. We determine spatially resolved absolute values of the magnetic penetration depth λ and study its temperature dependence as the system undergoes magnetic phase transitions from paramagnetic to antiferromagnetic, and to weak ferromagnetic, all within the superconducting regime. We estimate the absolute pinning force of Abrikosovmore » vortices, which shows a position dependence and temperature dependence as well, and discuss the possibility of the purported spontaneous vortex formation.« less

  18. Completion Report for the Well ER-6-2 Site Corrective Action Unit 97: Yucca Flat - Climax Mine

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2008-03-01

    Well ER-6-2 and its satellite hole, Well ER-6-2 No.1, were drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. Well ER-6-2 was drilled in two stages in 1993 and 1994; the satellite hole, Well ER-6-2 No.1 was drilled nearby in 1993 but was abandoned. The wells were drilled as part of a hydrogeologic investigation program for the Yucca Flat-Climax Mine Corrective Action Unit Number 97, in the northeastern portion of the Nevada Test Site. The wells are located in Yucca Flat, within Area 6 of the Nevada Test Site. The wells provided information regarding the radiological and hydrogeological environment in a potentially down-gradient position from tests conducted in northern and central Yucca Flat. Construction of Well ER-6-2 began with a 1.2-meter-diameter surface conductor hole, which was drilled and cased off to a depth of 30.8 meters below the surface. A 50.8-centimeter diameter surface hole was then rotary drilled to the depth of 578.5 meters and cased off to the depth of 530.4 meters. The hole diameter was then reduced to 27.0 centimeters, and the borehole was advanced to a temporary depth of 611.4 meters. The borehole was conventionally cored to a total depth of 1,045 meters with a diameter of 14.0 centimeters. Borehole sloughing required cementing and re-drilling of several zones. The open-hole completion accesses the lower carbonate aquifer, the CP thrust fault, and the upper clastic confining unit. A fluid level depth of 543.2 meters was most recently measured in the open borehole in September 2007. No radionuclides were encountered during drilling. The satellite hole Well ER-6-2 No.1 was drilled approximately 15.2 meters north of Well ER-6-2 on the same drill pad. This was planned to be used as an observation well during future hydrologic testing at Well ER-6-2; however, the satellite hole was abandoned at the depth of 399 meters due to stuck drill pipe, and was subsequently cemented to the surface. Detailed lithologic descriptions with stratigraphic assignments in this report are based on composite drill cuttings samples collected every 3 meters, cores taken between the depths of 619.3 and 1,042.4 meters, and geophysical log data. Stratigraphic assignments within the Paleozoic section are based on paleontological analyses. The well was collared in alluvium and at 30.8 meters penetrated Paleozoic carbonate rocks. These consisted of dolostone with minor shale and limestone of the Bonanza King Formation, and limestone with minor quartzite, sandstone, and dolostone assigned to the Guilmette Formation. The borehole reached total depth in a shale unit assigned to the Chainman Shale. The units below the Bonanza King Formation are overturned due to faulting and folding and, therefore, are stratigraphically upside-down.

  19. Microsoft Word - DOE Final Report 2013 - GTL ER64516-1031199-0013966 Chisholm-Polz-Alm.doc

    Office of Scientific and Technical Information (OSTI)

    DOE award: ER64516-1031199-0013966 2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus and Vibrio PIs Sallie Chisholm, Eric Alm and Martin Polz MIT Our overarching goal with this proposal was to develop a deep understanding of the design of Prochlorococcus and Vibrio cells, the variations in their designs, and the constraints that have shaped this variation at the cell-environment interface. That is, we wanted to develop our

  20. Audit of Work Force Restructuring at the Fernald Environmental Management Project, ER-B-96-01

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    U.S. Department of Energy Office of Inspector General AUDIT OF WORK FORCE RESTRUCTURING AT THE FERNALD ENVIRONMENTAL MANAGEMENT PROJECT Report Number: ER-B-96-01 Eastern Regional Audit Office Date of Issue: April 23, 1996 Oak Ridge, Tennessee 37830 AUDIT OF WORK FORCE RESTRUCTURING AT THE FERNALD ENVIRONMENTAL MANAGEMENT PROJECT TABLE OF CONTENTS Page SUMMARY . . . . . . . . . . . . . . . . . . . . . 1 PART I - APPROACH AND OVERVIEW 3 Introduction . . . . . . . . . . . . . . . 3 Scope and

  1. ER2 Instrumentation and Measurements for CLASIC (Cloud Land Surface Interaction Campaign) June-2007 SGP {Author-Jimmy Voyles}

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ER2 Desired Measurements for CLASIC June 2007 SGP May 31, 2007 1 MEASUREMENT SOURCE DESIRED MEASUREMENTS AND PRODUCTS INSTRUMENT SYSTEMS Cloud Radar System (CRS), W-Band (95 GHz) 1) Vertical profiles of calibrated radar reflectivity 2) Vertical profiles of Doppler velocity 3) Vertical profiles of estimated IWC 4) Vertical profiles of linear depolarization ratio 5) (ASCII files and quick look plots for all of the above quantities) Resolution: 5 sec resolution? Cloud Physics Lidar (CPL) (1064,

  2. Close-Out Report of DOE Grant DE-FG02-91ER40682 for November 1, 2009 - April 30, 2013

    SciTech Connect (OSTI)

    Paulini, Manfred

    2013-07-20

    This document is the close-out report of Grant DE-FG02-91ER40682 from the Carnegie Mellon University (CMU) High-Energy Physics (HEP) Group to the U.S. Department of Energy (DOE).

  3. Completion Report for Model Evaluation Well ER-5-5: Corrective Action Unit 98: Frenchman Flat

    SciTech Connect (OSTI)

    NSTec Underground Test Area and Boreholes Programs and Operations

    2013-01-18

    Model Evaluation Well ER-5-5 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of Nevada Environmental Management Operations at the Nevada National Security Site (formerly known as the Nevada Test Site). The well was drilled in July and August 2012 as part of a model evaluation well program in the Frenchman Flat area of Nye County, Nevada. The primary purpose of the well was to provide detailed geologic, hydrogeologic, chemical, and radiological data that can be used to test and build confidence in the applicability of the Frenchman Flat Corrective Action Unit flow and transport models for their intended purpose. In particular, this well was designed to obtain data to evaluate the uncertainty in model forecasts of contaminant migration from the upgradient underground nuclear test MILK SHAKE, conducted in Emplacement Hole U-5k in 1968, which were considered to be uncertain due to the unknown extent of a basalt lava-flow aquifer present in this area. Well ER-5-5 is expected to provide information to refine the Phase II Frenchman Flat hydrostratigraphic framework model, if necessary, as well as to support future groundwater flow and transport modeling. The 31.1-centimeter (cm) diameter hole was drilled to a total depth of 331.3 meters (m). The completion string, set at the depth of 317.2 m, consists of 16.8-cm stainless-steel casing hanging from 19.4-cm carbon-steel casing. The 16.8-cm stainless-steel casing has one slotted interval open to the basalt lava-flow aquifer and limited intervals of the overlying and underlying alluvial aquifer. A piezometer string was also installed in the annulus between the completion string and the borehole wall. The piezometer is composed of 7.3-cm stainless-steel tubing suspended from 6.0-cm carbon-steel tubing. The piezometer string was landed at 319.2 m, to monitor the basalt lava-flow aquifer. Data collected during and shortly after hole construction include composite drill cuttings samples collected every 3.0 m, various geophysical logs, preliminary water quality measurements, and water-level measurements. The well penetrated 331.3 m of Quaternary–Tertiary alluvium, including an intercalated layer of saturated basalt lava rubble. No well development or hydrologic testing was conducted in this well immediately after completion; however, a preliminary water level was measured in the piezometer string at the depth of 283.4 m on September 25, 2012. No tritium above the minimum detection limit of the field instruments was detected in this hole. Future well development, sampling, and hydrologic testing planned for this well will provide more accurate hydrologic information for this site. The stratigraphy, general lithology, and water level were as expected, though the expected basalt lava-flow aquifer is basalt rubble and not the dense, fractured lava as modeled. The lack of tritium transport is likely due to the difference in hydraulic properties of the basalt lava-flow rubble encountered in the well, compared to those of the fractured aquifer used in the flow and transport models.

  4. Completion Report for Well ER-12-3 Corrective Action Unit 99: Rainier Mesa - Shoshone Mountain

    SciTech Connect (OSTI)

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Bechtel Nevada Corporation

    2006-05-01

    Well ER-12-3 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The well was drilled in March and April 2005 as part of a hydrogeologic investigation program for the Rainier Mesa-Shoshone Mountain Corrective Action Unit. The overall purpose of the well was to gather subsurface data to better characterize the hydrogeology of central Rainier Mesa, especially in the older Tertiary volcanic rocks and Paleozoic sedimentary rocks. The main 47.0-centimeter hole was drilled to a depth of 799.2 meters and cased with 33.97-centimeter casing to 743.1 meters. The hole diameter was then decreased to 31.1 centimeters, and the well was drilled to a total depth of 1,496.0 meters. The completion string consisted of 13.97-centimeter stainless steel casing, with two slotted intervals open to the lower carbonate aquifer, suspended from 19.37-centimeter carbon steel casing. A piezometer string was installed outside the 33.97-centimeter casing to a depth of 467.1 meters to monitor a zone of perched water within the Tertiary volcanic section. Data gathered during and shortly after hole construction include composite drill cuttings samples collected every 3 meters (extra cuttings samples were collected from the Paleozoic rocks for paleontological analyses), sidewall core samples from 35 depths, various geophysical logs, and water level measurements. These data indicate that the well penetrated 674.2 meters of Tertiary volcanic rocks and 821.7 meters of Paleozoic dolomite and limestone. Forty-nine days after the well was completed, but prior to well development and testing, the water level inside the main hole was tagged at the depth of 949.1 meters, and the water level inside the piezometer string was tagged at 379.9 meters.

  5. Idaho National Engineering Laboratory (INEL) Environmental Restoration (ER) Program Baseline Safety Analysis File (BSAF)

    SciTech Connect (OSTI)

    1995-09-01

    The Baseline Safety Analysis File (BSAF) is a facility safety reference document for the Idaho National Engineering Laboratory (INEL) environmental restoration activities. The BSAF contains information and guidance for safety analysis documentation required by the U.S. Department of Energy (DOE) for environmental restoration (ER) activities, including: Characterization of potentially contaminated sites. Remedial investigations to identify and remedial actions to clean up existing and potential releases from inactive waste sites Decontamination and dismantlement of surplus facilities. The information is INEL-specific and is in the format required by DOE-EM-STD-3009-94, Preparation Guide for U.S. Department of Energy Nonreactor Nuclear Facility Safety Analysis Reports. An author of safety analysis documentation need only write information concerning that activity and refer to BSAF for further information or copy applicable chapters and sections. The information and guidance provided are suitable for: {sm_bullet} Nuclear facilities (DOE Order 5480-23, Nuclear Safety Analysis Reports) with hazards that meet the Category 3 threshold (DOE-STD-1027-92, Hazard Categorization and Accident Analysis Techniques for Compliance with DOE Order 5480.23, Nuclear Safety Analysis Reports) {sm_bullet} Radiological facilities (DOE-EM-STD-5502-94, Hazard Baseline Documentation) Nonnuclear facilities (DOE-EM-STD-5502-94) that are classified as {open_quotes}low{close_quotes} hazard facilities (DOE Order 5481.1B, Safety Analysis and Review System). Additionally, the BSAF could be used as an information source for Health and Safety Plans and for Safety Analysis Reports (SARs) for nuclear facilities with hazards equal to or greater than the Category 2 thresholds, or for nonnuclear facilities with {open_quotes}moderate{close_quotes} or {open_quotes}high{close_quotes} hazard classifications.

  6. Long-range ferromagnetic order induced by a donor impurity band exchange in SnO{sub 2}:Er{sup 3+} nanoparticles

    SciTech Connect (OSTI)

    Aragn, F. H.; Coaquira, J. A. H.; Chitta, V. A.; Hidalgo, P.; Brito, H. F.

    2013-11-28

    In this work, the structural and magnetic properties of Er-doped SnO{sub 2} (SnO{sub 2}:Er) nanoparticles are reported. The SnO{sub 2}:Er nanoparticles have been synthesized by a polymer precursor method with Er content from 1.0% to 10.0%. X-ray diffraction results indicate the formation of only the rutile-type structure in all samples. The estimated mean crystallite size shows a decrease from ?10 to ?4?nm when the Er content is increased from 1.0% to 10.0%. The particle size values have been corroborated by transmission electron microscopy technique. The thermal dependence of the magnetization is consistent with the 3+ oxidation state of erbium ions for all samples. A strong paramagnetic-like behavior coexisting with a ferromagnetic phase has been determined for samples with Er content below 5.0%. Above this concentration, only a paramagnetic behavior has been determined. Isothermal magnetization curves are consistent with the occurrence of long-range ferromagnetic order mediated by donor electrons forming bound magnetic polarons which overlap to produce a spin-split impurity band.

  7. Completion Report for Well ER-16-1 Corrective Action Unit 99: Rainier Mesa - Shoshone Mountain

    SciTech Connect (OSTI)

    NSTec Geology Services

    2006-12-01

    Well ER-16-1 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The well was drilled in June and July 2005 as part of a hydrogeologic investigation program for the Rainier Mesa-Shoshone Mountain Corrective Action Unit, Number 99. The overall purpose of the well was to gather subsurface data to better characterize the hydrogeology of the Shoshone Mountain area, especially in the older Tertiary and pre-Tertiary strata. The main 46.99-centimeter hole was drilled to a depth of 702.9 meters and cased with 33.97-centimeter casing to 663.7 meters. The hole diameter was then decreased to 31.1 centimeters, and the well was drilled to total depth of 1,220.7 meters. A completion string set at the depth of 1,162.4 meters consisted of 13.97-centimeter stainless-steel casing, with one continuous slotted interval open to the lower carbonate aquifer. The fluid level in the borehole soon dropped, so the borehole was deepened in July 2006. To deepen the borehole, the slotted section was cemented and a 12.1-centimeter hole was drilled through the bottom of the completion string to the new total depth of 1,391.7 meters, which is 171.0 meters deeper than the original borehole. A string of 6.03-centimeter carbon-steel tubing with one continuous slotted interval at 1,361.8 to 1,381.4 meters, and open to the lower carbonate aquifer, was installed in the well with no gravel packing or cement, to serve as a monitoring string. Data gathered during and shortly after hole construction include composite drill cuttings samples collected every 3 meters (extra cuttings samples were collected from the Paleozoic rocks for paleontological analyses), sidewall core samples from 37 depths, various geophysical logs, and water level measurements. These data indicate that the well penetrated 646.8 meters of Tertiary volcanic rocks and 744.9 meters of Paleozoic dolomite, quartzite, shale, and limestone. Three weeks after the monitoring string was installed, the water level was tagged at the drill hole depth of 1,271.9 meters, which equates to an estimated elevation of 761.7 meters, accounting for the borehole angle.

  8. Technical support to the ER program subsurface technologies team leader. Final report, March 15, 1993--March 15, 1998

    SciTech Connect (OSTI)

    1998-12-01

    This research included development of a new geologic sample management facility and associated quality assurance systems for the LANL Environmental Restoration Program. Additional work with the LANL Environmental Restoration Program included the development of Sampling and Analysis Plans (SAP) for various Operable Units for the Laboratory. The PI (Davidson) served as the sample curation/sample management specialist on the ER program Subsurface Studies Technical Team. Specialization in Field Unit Data Base systems was the focus of the work towards the end of the contract. A document is included which provides the Statement of Policy for the management of borehole samples collected during environmental restoration activities at LANL.

  9. FinaI Technical Report for DOE grant DE-FG02-91ER20038 PI: Timothy Nelson

    Office of Scientific and Technical Information (OSTI)

    FinaI Technical Report for DOE grant DE-FG02-91ER20038 PI: Timothy Nelson Yale University "Spatial Regulation of C4 Genes in C3, C4 and C3/C4 Intermediate Flaveria Species" AIMS The long-range goal of this project was to understand the control of plant cell differentiation in spatial patterns, using the differentiation of BS and M cells surrounding veins of C4 leaves as a model. Based on our prior studies on the structure and regulation of BS- and M-specific C4 genes and on cellular

  10. Final Final Scientific_tech_reprt_closeout DE-FG02-05ER46223 02.18.15

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    SCIENTIFIC/TECHNICAL REPORT 1. Grant details a Office of Science/ Department of Energy b DE-FG02-05ER46223, University of Pennsylvania c. Modular Designed Proteins Constructions for Solar Generated H2 from Water, d. P. Leslie Dutton (PI), Director Johnson Research Fdn., Univ. of Pennsylvania, Stellar-Chance Labs, Ste 1005, 422 Curie Blvd., Philadelphia, PA 19104-6059; dutton@ mail.med.upenn.edu; 215-898-0991 e. Submitting Official, title, and contact information: P. Leslie Dutton (PI), Director

  11. Completion Report for Well ER-20-4 Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2011-04-30

    Well ER-20-4 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada National Security Site, Nye County, Nevada. The well was drilled in August and September 2010 as part of the Pahute Mesa Phase II drilling program. The primary purpose of the well was to investigate the possibility of radionuclide transport from up-gradient underground nuclear tests conducted in central Pahute Mesa. This well also provided detailed hydrogeologic information in the Tertiary volcanic section that will help reduce uncertainties within the Pahute Mesa-Oasis Valley hydrostratigraphic framework model.

  12. Increased efficiency in multijunction solar cells through the incorporation of semimetallic ErAs nanoparticles into the tunnel junction

    SciTech Connect (OSTI)

    Zide, J.M.O.; Kleiman-Shwarsctein, A.; Strandwitz, N.C.; Zimmerman, J.D.; Steenblock-Smith, T.; Gossard, A.C.; Forman, A.; Ivanovskaya, A.; Stucky, G.D.

    2006-04-17

    We report the molecular beam epitaxy growth of Al{sub 0.3}Ga{sub 0.7}As/GaAs multijunction solar cells with epitaxial, semimetallic ErAs nanoparticles at the interface of the tunnel junction. The states provided by these nanoparticles reduce the bias required to pass current through the tunnel junction by three orders of magnitude, and therefore drastically reduce the voltage losses in the tunnel junction. We have measured open-circuit voltages which are 97% of the sum of the constituent cells, which result in nearly double the efficiency of our multijunction cell with a conventional tunnel junction.

  13. FInal Report: DE-FG02-04ER41310 "Elementary Particle Physics"

    SciTech Connect (OSTI)

    Izen, Joseph M.; Ishak-Boushaki, Mustapha

    2013-10-18

    ATLAS and the LHC are delivering on the promise of discovery physics at the high energy frontier. Using 4.8 fb^-1 of 2011 √s=7 TeV data and the first 5.8 fb?1 of 2012 √s=8 TeV data, ATLAS published the observation of a new particle with a mass of 126 GeV with a significance of 5.9σ that is compatible with a Standard Model (SM) Higgs. The LHC is outperforming intial projections for the 2012 run, and ATLAS is on track to integrate ~20 fb^(-1) of proton-proton collisions in 2012 before Long Shutdown 1 (LS1) begins in Spring 2013. University of Texas at Dallas (UTD) physicists will complete work on two ATLAS analyses this fall. The first is the search for the gauge bosons of a hypothesized dark sector. For 2011 data, UTD is responsible for the ?dark photon?search in the electron-jet channel, and we are looking forward to an expanded leadership role in the dark photon search using the full 2012 data set. Our second analysis interest is the study of X/Y/Z exotic states having cc ̅ content, which builds on our experience in this field from the BABAR experiment After completing a measurement of the Xc(3872) production cross section this fall, we will search for evidence of the Z(4430)+ which is reported by Belle but not confirmed by BABAR. The UTD group has played a strong role in ATLAS operations, with group members serving as Pixel Run Coordinator, ATLAS Shift Leader, and Pixel/Inner Detector Shifter. For most of the current 3-year funding cycle, a group member coordinated the development of the Pixel DAQ code, and another continues to build and maintain the data quality monitoring (DQM) application that is used by the Inner Detector control room shifter. Additionally, members of our group take Pixel on-call expert shifts for DQM and DAQ. We led an optoboard lifetime study to assess concerns of premature on-detector VCSEL failure using the Pixel working prototype detector at CERN. Physicists based at UTD participated through Pixel Offline DQM and ATLAS Distributed Computing Operations Shifts (ADCoS) During Summer 2012, UTD joined upgrade activities in preparation for LS1. We took a major role in the testing of Electro-Readout (ER) Bundle testing for new Service Quarter Panels (nSQP?s), and we developed two utilities to measure the timing jitter and bit error rate of the Pixel readout chain for use commissioning Pixel detector upgrades. During BABAR?s heyday, the UTD group pioneered the use of e^+ e^- annihilation events with hard Initial State Radiation (ISR) to study the charm threshold region, and we carried out the first BABAR double-cc ̅ analysis. Our most recent ISR paper, written in collaboration with A. Palano (Bari) is Exclusive Production of Ds^+ Ds^-, D_S^(*+) Ds^-, and Ds^(*+) Ds^(*-) via e+ e- Annihilation with Initial-State-Radiation was published in Physical Review D 82, 052004 (2010). Work continues on a study of ISR Λc^+ Λc^- production, and a new search to establish and study double-ss ̅ production is starting.

  14. Completion Report for Well ER-EC-14, Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    2013-03-05

    Well ER-EC-14 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Management Operations Underground Test Area (UGTA) Activity at the Nevada National Security Site (NNSS; formerly Nevada Test Site), Nye County, Nevada. The well was drilled in September and October 2012, as part of the Central and Western Pahute Mesa Corrective Action Unit Phase II drilling program. The primary purpose of the well was to provide detailed hydrogeologic information for the Fortymile Canyon composite hydrostratigraphic unit in the Timber Mountain moat area, within the Timber Mountain caldera complex, that will help address uncertainties within the Pahute MesaOasis Valley hydrostratigraphic framework model. The main 55.9-centimeter (cm) hole was drilled to a total depth of 325.5 meters (m) and cased with 40.6-cm casing to 308.1 m. The hole diameter was then decreased to 37.5 cm, and drilling continued to a total depth of 724.8 m. The completion casing string, set to the depth of 690.9 m, consists of 16.8-cm stainless-steel casing hanging from 19.4-cm carbon-steel casing. The stainless-steel casing has two slotted intervals open to the Rainier Mesa Tuff. Two piezometer strings were installed in Well ER-EC-14. Both piezometer strings, each with one slotted interval, consist of 6.0-cm carbon-steel tubing at the surface, then cross over to 7.3-cm stainless-steel tubing just above the water table. The shallow piezometer string was landed at 507.8 m, and the deep piezometer string was landed at 688.6 m. Both piezometer strings are set to monitor groundwater within moderately to densely welded Rainier Mesa Tuff. Data collected during and shortly after hole construction include composite drill cuttings samples collected every 3.0 m, various geophysical logs, water quality (including tritium and other radionuclides) measurements, and water level measurements. The well penetrated 15.2 m of alluvium and 709.6 m of Tertiary volcanic rocks. The stratigraphy and general lithology were not as expected due to the position of Well ER-EC-14 relative to the buried caldera margins of the Timber Mountain caldera complex. The well is located inside the Rainier Mesa caldera, but outside the younger Ammonia Tanks caldera. On November 5, 2012, a preliminary fluid level in the shallow piezometer string was measured at the depth of 311.8 m. This water level depth was taken before installation of the bridge plug (to be placed within the main completion casing to separate the two slotted zones). Well development, hydrologic testing, and sampling, will be conducted at a later date. No tritium above levels detectable by field methods were encountered in this hole. All Fluid Management Plan (FMP) requirements for Well ER-EC-14 were met. Analysis of monitoring samples and FMP confirmatory samples indicated that fluids generated during drilling at Well ER-EC-14 met the FMP criteria for discharge to an unlined sump or designated infiltration area. All sanitary and hydrocarbon waste generated was properly handled and disposed of.

  15. In-situ time-of-flight neutron diffraction of ErD2 (beta phase) formation during D2 loading.

    SciTech Connect (OSTI)

    Browning, James Frederick; Llobet, Anna; Snow, Clark Sheldon; Rodriguez, Mark Andrew; Wixom, Ryan R.

    2008-06-01

    In an effort to better understand the structural changes occurring during hydrogen loading of erbium target materials, we have performed D{sub 2} loading of erbium metal (powder) with simultaneous neutron diffraction analysis. This experiment tracked the conversion of Er metal to the {alpha} erbium deuteride (solid-solution) phase and then on to the {beta} (fluorite) phase. Complete conversion to ErD{sub 2.0} was accomplished at 10 Torr D{sub 2} pressure with deuterium fully occupying the tetrahedral sites in the fluorite lattice. Increased D{sub 2} pressure (up to 500 Torr at 450 C) revealed {approx}10 % deuterium occupation of the octahedral sites. Subsequent vacuum pumping of the sample at 450 C removed octahedral site occupancy while maintaining tetrahedral deuterium occupancy, thereby yielding stoichiometric ErD{sub 2.0} {beta} phase.

  16. Magnetic properties and magnetocaloric effects of Gd{sub x}Er{sub 1−x}Ga (0 ≤ x ≤ 1) compounds

    SciTech Connect (OSTI)

    Zheng, X. Q.; Wang, L. C.; Wu, R. R.; Hu, F. X.; Sun, J. R.; Shen, B. G.; Chen, J.

    2014-05-07

    We carefully studied the magnetic properties and magnetocaloric effect of Gd{sub x}Er{sub 1-x}Ga (0 ≤ x ≤ 1) compounds. The Gd{sub x}Er{sub 1-x}Ga compounds undergo two magnetic transitions with temperature increasing: spin-reorientation or antiferromagnetic-to-ferromagnetic (FM) transition and FM-to-paramagnetic transition. As the content of Gd increases from 0 to 1, the transition temperature in low temperature region changes from 15 K to 66 K and the Curie temperature increases obviously from 30 K to 181.9 K. Although the maximum value of magnetic entropy change (ΔS{sub M}) for Gd{sub x}Er{sub 1−x}Ga decreases with the increase of x, the refrigerant capacity (RC) improves remarkably compared with that of ErGa compound. Table-like ΔS{sub M} curves are observed for the compounds with x = 0.1, 0.2, 0.3, and 0.4, which are very useful for real cooling applications. And Gd{sub 0.2}Er{sub 0.8}Ga and Gd{sub 0.3}Er{sub 0.7}Ga compounds show better magnetocaloric features than others in this series under considerations of both ΔS{sub M} and RC. The results of this series of compounds show us a possible way to design and improve the magnetic refrigerant materials by making some substitutions.

  17. DE-FG02-08ER64658 (OASIS) - Final Technical Report

    SciTech Connect (OSTI)

    Sharman, Jonathan

    2013-09-05

    Project OASIS (Operation of Advanced Structures, Interfaces and Sub-components for MEAs) was a 12 month project that ran from 1st September 2008 to 31st August 2009, and was managed by the Department of Energy Office of Science, Chicago Office, as Award No DE-FG02-08ER64658, with Johnson Matthey Fuel Cells Inc. as the sole contractor. The project was completed on schedule, with technical successes (details below) and payment of the full grant award made by DOE. The aim of the project was the development of membrane electrode assemblies (MEAs) for H2/air polymer electrolyte membrane (PEM) fuel cells that would give higher performance under hot/dry and dry operating conditions, ideally with no loss of performance under wet conditions. Reducing or eliminating the need for humidifying the incoming gases will allow significant system cost and size reduction for many fuel cell applications including automotive, stationary and back-up power, and portable systems. Portable systems are also of particular interest in military markets. In previous work Johnson Matthey Fuel Cells had developed very stable, corrosion-resistant catalysts suitable for resisting degradation by carbon corrosion in particular. These materials were applied within the OASIS project as they are considered necessary for systems such as automotive where multiple start-stop events are experienced. These catalysts were contrasted with more conventional materials in the design of catalyst layers and novel microporous layers (MPLs) and gas diffusion layer (GDL) combinations were also explored. Early on in the work it was shown how much more aggressive high temperature operation is than dry operation. At the same humidity, tests at 110?C caused much more dehydration than tests at 80?C and the high temperature condition was much more revealing of improvements made to MEA design. Alloy catalysts were introduced and compared with Pt catalysts with a range of particle sizes. It was apparent that the larger particle sizes of the alloy catalysts led to a reduction in performance that offset much of their kinetic advantage. The Pt-only materials clearly showed that small particles are beneficial to good performance under hot/dry conditions, because of their higher surface area, although they are known to be less stable to cyclic operation. An ex-situ water vapour sorption technique was developed that showed a very clear correlation with in-cell performance: catalyst powders that absorbed more water gave better performance in-cell. It was shown that alloy catalysts could give a 25 mV advantage over Pt-only at 1 Acm-2. GDL design was also shown to influence performance and more permeable GDLs on the anode allowed better membrane hydration and therefore conductivity. A very impermeable GDL on the cathode caused cathode flooding even under dry conditions, but a novel cathode MPL incorporating ionomer and operating at 110?C, 33/17% RH showed a 150 mV gain at 800 mAcm-2 over the conventional MPL. This project has increased the understanding of the factors that influence performance loss under dry conditions, including the development of an insightful ex-situ characterisation technique (Dynamic Vapour Sorption). All the approaches investigated can be readily implemented in state-of the-art MEAs, although optimisation would be needed to integrate the new designs with existing MEA types and to tune to the exact range of operating conditions. The work is thus expected to benefit the public by feeding through more condition-tolerant production MEAs to a range of applications and thereby accelerate the commercialisation of fuel cell technology. In summary, a number of specific catalyst, catalyst layer, MPL and GDL improvements were made during this project. Often the best designs under dry conditions translated to some performance loss under wet conditions, but compromise situations were also found where dry performance was improved with no loss of wet performance.

  18. Completion Report for Well ER-EC-12 Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2011-04-30

    Well ER-EC-12 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada National Security Site (formerly known as the Nevada Test Site), Nye County, Nevada. The well was drilled in June and July 2010 as part of the Pahute Mesa Phase II drilling program. The primary purpose of the well was to provide detailed hydrogeologic information in the Tertiary volcanic section in the area between Pahute Mesa and the Timber Mountain caldera complex that will help address uncertainties within the Pahute MesaOasis Valley hydrostratigraphic model. In particular, the well was intended to help define the structural position and hydraulic parameters for volcanic aquifers potentially down-gradient from historic underground nuclear tests on Pahute Mesa. It may also be used as a long-term monitoring well.

  19. Magnetic and dielectric behavior of the spin-chain compound Er?BaNiO? well below its Nel temperature

    SciTech Connect (OSTI)

    Basu, Tathamay; Singh, Kiran; Sampathkumaran, E. V.; Mohapatra, N.

    2014-09-21

    We have recently reported that the Haldane spin-chain system, Er?BaNiO?, undergoing antiferromagnetic order below (T{sub N}=) 32 K, is characterized by the onset of ferroelectricity near 60 K due to magnetoelectric coupling induced by short-range magnetic-order within spin-chains. We have carried out additional magnetic and dielectric studies to understand the properties well below T{sub N}. We emphasize here on the following: (i) A strong frequency dependent behaviors of ac magnetic susceptibility and complex dielectric properties have been observed at much lower temperatures (<8 K), that is, reentrant multiglass-like phenomenon, naturally suggesting the existence of an additional transition well below T{sub N}. (ii) Magnetoelectric phase coexistence is observed at very low temperature (e.g., T=2 K), where the high-field magnetoelectric phase is partially arrested on returning to zero magnetic field after a cycling through metamagnetic transition.

  20. Completion Report for Well ER-EC-13 Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2011-05-31

    Well ER-EC-13 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada National Security Site (formerly Nevada Test Site), Nye County, Nevada. The well was drilled in October 2010 as part of the Pahute Mesa Phase II drilling program. A main objective was to provide detailed hydrogeologic information for the Fortymile Canyon composite unit hydrostratigraphic unit in the Timber Mountain moat area, within the Timber Mountain caldera complex, that will help address uncertainties within the Pahute MesaOasis Valley hydrostratigraphic framework model. This well may also be used as a long-term monitoring well.

  1. Completion Report for Well ER-EC-15 Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2011-05-31

    Well ER-EC-15 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada National Security Site (formerly known as the Nevada Test Site), Nye County, Nevada. The well was drilled in October and November 2010, as part of the Pahute Mesa Phase II drilling program. The primary purpose of the well was to provide detailed hydrogeologic information in the Tertiary volcanic section in the area between Pahute Mesa and the Timber Mountain caldera complex that will help address uncertainties within the Pahute MesaOasis Valley hydrostratigraphic model. In particular, the well was intended to help define the structural position and hydraulic parameters of volcanic aquifers potentially down-gradient from underground nuclear tests on Pahute Mesa. It may also be used as a long-term monitoring well.

  2. Completion Report for Well ER-12-4, Corrective Action Unit 99: Rainier Mesa - Shoshone Mountain (includes Errata Sheet)

    SciTech Connect (OSTI)

    U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office; Bechtel Nevada

    2006-05-01

    Well ER-12-4 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. This well was drilled in May 2005, as part of a hydrogeologic investigation program for the Rainier Mesa-Shoshone Mountain Corrective Action Unit in the north-central portion of the Nevada Test Site. The well is located on Rainier/Aqueduct Mesa, northwest of Yucca Flat, within Area 12 of the Nevada Test Site. The well provided information regarding the radiological and physical environment near underground nuclear tests conducted in U12t Tunnel, information on the pre-Tertiary rocks in the area, and depth to the regional water table.

  3. Well Completion Report for Well ER-20-11, Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2013-02-27

    Well ER-20-11 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Management Operations Underground Test Area (UGTA) Activity at the Nevada National Security Site (formerly Nevada Test Site), Nye County, Nevada. The well was drilled in September 2012 as part of the Central and Western Pahute Mesa Corrective Action Unit Phase II drilling program. Well ER-20-11 was constructed to further investigate the nature and extent of radionuclidecontaminated groundwater encountered in two nearby UGTA wells, to help define hydraulic and transport parameters for the contaminated Benham aquifer, and to provide data for the UGTA hydrostratigraphic framework model. The 44.5-centimeter (cm) surface hole was drilled to a depth of 520.0 meters (m) and cased with 34.0-cm casing to 511.5 m. The hole diameter was then decreased to 31.1 cm, and the borehole was drilled to a total depth of 915.6 m. The hole was completed to allow access for hydrologic testing and sampling in the target aquifer, which is a lava-flow aquifer known as the Benham aquifer. The completion casing string, set to the depth of 904.3 m, consists of a string of 6?-inch (in.) stainless-steel casing hanging from a string of 7?-in. carbon-steel casing. The stainless-steel casing has one slotted interval at 796.3 to 903.6 m. One piezometer string was installed, which consists of 2?-in. stainless-steel tubing that hangs from 2?-in. carbon-steel tubing via a crossover sub. This string was landed at 903.8 m and is slotted in the interval 795.3 to 903.1 m. Data collected during and shortly after hole construction include composite drill cuttings samples collected every 3.0 m, various geophysical logs, fluid samples (for groundwater chemistry analysis and tritium measurements), and water-level measurements. The well penetrated 915.6 m of Tertiary volcanic rock, including one saturated lava flow aquifer. Measurements on samples taken from the undeveloped well indicated elevated tritium levels within the Benham aquifer. The maximum tritium level measured with field equipment was 146,131 picocuries per liter from a sample obtained at the depth of 912.0 m. The fluid level was measured in the piezometer string at a depth of 504.5 m on September 26, 2012. All Fluid Management Plan (FMP) requirements for Well ER-20-11 were met. Analysis of monitoring samples and FMP confirmatory samples indicated that fluids generated during drilling at Well ER-20-11 met the FMP criteria for discharge to an unlined sump or designated infiltration area. Well development, hydrologic testing, and sampling will be conducted at a later date.

  4. Relativistic energy density functionals: Low-energy collective states of {sup 240}Pu and {sup 166}Er

    SciTech Connect (OSTI)

    Li, Z. P.; Niksic, T.; Vretenar, D.; Ring, P.; Meng, J.

    2010-06-15

    The empirical relativistic density-dependent, point-coupling energy density functional, adjusted exclusively to experimental binding energies of a large set of deformed nuclei with Aapprox =150-180 and Aapprox =230-250, is tested with spectroscopic data for {sup 166}Er and {sup 240}Pu. Starting from constrained self-consistent triaxial relativistic Hartree-Bogoliubov calculations of binding energy maps as functions of the quadrupole deformation in the beta-gamma plane, excitation spectra and E2 transition probabilities are calculated as solutions of the corresponding microscopic collective Hamiltonian in five dimensions for quadrupole vibrational and rotational degrees of freedom and compared with available data on low-energy collective states.

  5. Synthesis and characterization of R{sub 2}MnTiO{sub 7} (R = Y and Er) pyrochlores oxides

    SciTech Connect (OSTI)

    Martnez-Coronado, R.; Alonso, J.A.; Fernndez, M.T.

    2013-09-01

    Graphical abstract: - Abstract: New pyrochlore-like phases of composition R{sub 2}MnTiO{sub 7} (R = Er and Y) have been synthesized by a soft-chemistry procedure involving citrates of the different metal ions followed by thermal treatments at moderate temperatures (850 C for 12 h in air). A characterization by X-ray diffraction and neutron powder diffraction (NPD) has been carried out in order to determine the crystal structure features: these phyrochlores are cubic, space group Fd-3m, defining an intrinsically frustrated three-dimensional system. The Rietveld-refinement from NPD data at room temperature evidences an antisite cation disorder (distribution of Mn between A and B positions) that is accompanied by an increment of the oxygen-vacancy concentration due to the reduction of Mn{sup 4+} at the B position to Mn{sup 2+} at the A position. Thermogravimetric analysis (TGA) was useful to evaluate the stability of these oxides in reducing conditions up to 500 C. Magnetic susceptibility measurements indicate a ferromagnetic behavior, due to the random distribution of Mn{sup 4+} ions in the octahedral sublattice. At lower temperatures there is a polarization of the R{sup +3} magnetic moments, which also participate in the magnetic structure. Aiming to evaluate these materials as possible electrodes for solid oxide fuel cells (SOFC) we determined that the thermal expansion coefficients between 100 and 900 C perfectly match with those of the usual electrolytes; however, these pyrochlore oxides display a semiconductor-like behavior with poor conductivity values, e.g. 6 10{sup ?3} cm{sup ?1} at 850 C for Er, which would prevent its use as MIEC (mixed ionic-electronic conductors) oxides in SOFC devices.

  6. Final Report for Grant DE-FG02-91ER40690 for the period 12/1/2010 to 4/30/2014

    SciTech Connect (OSTI)

    Durkin, Stan; Hill, Chris; Kass, Richard; Braaten, Eric; Mathur, Samir; Raby, Stuart; Shigemitsu, Junko; Gan, K. K.; Kagan, Harris; Hughes, Richard E.; Winer, Brian L.; Honscheid, Klaus

    2014-07-22

    This is the #12;final report for The Ohio State University high energy physics grant DE-FG02- 91ER40690. The activities of the various Tasks are briefy summarized over the previous grant period. The support from the Department of Energy is greatly appreciated.

  7. Audit of Economic Development Grants and a Cooperative Agreement with East Tennessee Not-For-Profit Organizations, ER-B-97-01

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AUDIT OF ECONOMIC DEVELOPMENT GRANTS AND A COOPERATIVE AGREEMENT WITH EAST TENNESSEE NOT-FOR-PROFIT ORGANIZATIONS Report Number: ER-B-97-01 Eastern Regional Audit Office Date of Issue: October 22, 1996 AUDIT OF ECONOMIC DEVELOPMENT GRANTS AND A COOPERATIVE AGREEMENT WITH EAST TENNESSEE NOT-FOR-PROFIT ORGANIZATIONS TABLE OF CONTENTS Page SUMMARY..................................................... 1 PART I - APPROACH AND OVERVIEW............................. 3

  8. Up-regulation of K{sub ir}2.1 by ER stress facilitates cell death of brain capillary endothelial cells

    SciTech Connect (OSTI)

    Kito, Hiroaki; Yamazaki, Daiju; Department of Biological Chemistry, Kyoto University, Graduate School of Pharmaceutical Sciences, Kyoto; Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Nagoya ; Ohya, Susumu; Yamamura, Hisao; Asai, Kiyofumi; Imaizumi, Yuji

    2011-07-29

    Highlights: {yields} We found that application of endoplasmic reticulum (ER) stress with tunicamycin to brain capillary endothelial cells (BCECs) induced cell death. {yields} The ER stress facilitated the expression of inward rectifier K{sup +} channel (K{sub ir}2.1) and induced sustained membrane hyperpolarization. {yields} The membrane hyperpolarization induced sustained Ca{sup 2+} entry through voltage-independent nonspecific cation channels and consequently facilitated cell death. {yields} The K{sub ir}2.1 up-regulation by ER stress is, at least in part, responsible for cell death of BCECs under pathological conditions. -- Abstract: Brain capillary endothelial cells (BCECs) form blood brain barrier (BBB) to maintain brain homeostasis. Cell turnover of BCECs by the balance of cell proliferation and cell death is critical for maintaining the integrity of BBB. Here we found that stimuli with tunicamycin, endoplasmic reticulum (ER) stress inducer, up-regulated inward rectifier K{sup +} channel (K{sub ir}2.1) and facilitated cell death in t-BBEC117, a cell line derived from bovine BCECs. The activation of K{sub ir} channels contributed to the establishment of deeply negative resting membrane potential in t-BBEC117. The deep resting membrane potential increased the resting intracellular Ca{sup 2+} concentration due to Ca{sup 2+} influx through non-selective cation channels and thereby partly but significantly regulated cell death in t-BBEC117. The present results suggest that the up-regulation of K{sub ir}2.1 is, at least in part, responsible for cell death/cell turnover of BCECs induced by a variety of cellular stresses, particularly ER stress, under pathological conditions.

  9. Completion Report for Well ER-20-7: Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Restoration

    2010-04-28

    Well ER-20-7 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office in support of the Nevada Environmental Restoration Project at the Nevada Test Site, Nye County, Nevada. The well was drilled in June 2009 as part of the Pahute Mesa Phase II drilling program. The primary purpose of the well was to further investigate migration of radionuclides from the nearby, up-gradient TYBO and BENHAM underground nuclear tests, which originally was discovered at Well Cluster ER-20-5. This well also provided detailed hydrogeologic information in the Tertiary volcanic section that will reduce uncertainties within the Pahute Mesa-Oasis Valley hydrostratigraphic framework model. The main 44.45-centimeter hole was drilled to a depth of 681.8 meters and cased with 33.97-centimeter casing to 671.7 meters. The hole diameter was then decreased to 31.12 centimeters, and the well was drilled to total depth of 894.9 meters. The completion string, set to the depth of 890.0 meters, consists of 14.13-centimeter stainless-steel casing hanging from 19.37-centimeter carbon-steel casing. The 14.13-centimeter stainless-steel casing has one continuous slotted interval open to the Topopah Spring aquifer. Data collected during and shortly after hole construction include composite drill cuttings samples collected every 3.0 meters, sidewall core samples from 20 depth intervals, various geophysical logs, water quality (primarily tritium) measurements, and water level measurements. The well penetrated 894.9 meters of Tertiary volcanic rock, including two saturated welded-tuff aquifers. A fluid level measurement was obtained during open-hole geophysical well logging for the upper, Tiva Canyon, aquifer at the depth of 615.7 meters on June 19, 2009. The fluid level measured in the open hole on June 27, 2009,after the total depth was reached and the upper aquifer was cased off, was also at the depth of 615.7 meters. Preliminary field measurements indicated 1.5 to 4.5 million picocuries per liter of tritium in the Tiva Canyon aquifer and 20 to 61 million picocuries per liter in the underlying Topopah Spring aquifer.

  10. Completion Report for Well ER-EC-11 Corrective Action Units 101 and 102: Central and Western Pahute Mesa

    SciTech Connect (OSTI)

    NSTec Environmental Management

    2010-12-01

    Well ER-EC-11 was drilled for the U.S. Department of Energy, National Nuclear Security Administration Nevada Site Office, in support of the Nevada Environmental Restoration Project at the Nevada National Security Site (formerly Nevada Test Site), Nye County, Nevada. The well was drilled in September and October 2009 as part of the Pahute Mesa Phase II drilling program. A main objective was to investigate radionuclide migration down-gradient from Well Cluster ER-20-5 and Well ER-20-7 and across the northern Timber Mountain moat structural zone into the area referred to as the Bench, between Pahute Mesa and the Timber Mountain caldera complex. A secondary purpose of the well was to provide detailed hydrogeologic information for the shallow- to intermediate-depth Tertiary volcanic section in the Bench area. This well also provided detailed hydrogeologic information in the Tertiary volcanic section to reduce uncertainties within the Pahute Mesa-Oasis Valley hydrostratigraphic framework model (Bechtel Nevada, 2002). The main 52.1-centimeter hole was drilled to a depth of 507.5 meters and then opened to a diameter of 66.0 centimeters. It was cased with 50.8-centimeter casing to 504.9 meters. The hole diameter was then decreased to 47.0 centimeters, and drilling continued to a total depth of 979.3 meters. It was then cased with 34.0-centimeter casing set at 965.5 meters. The hole diameter was then decreased to 31.1 centimeters and the borehole was drilled to a total depth of 1,264.3 meters. The completion casing string, set to the depth of 1,262.5 meters, consists of 19.4-centimeter stainless-steel casing hanging from 19.4-centimeter carbon-steel casing. The stainless-steel casing has two slotted intervals open to the Tiva Canyon and Topopah Spring aquifers. Four piezometer strings were installed in Well ER-EC-11. A string of carbon-steel 6.0-centimeter tubing with one slotted interval was inserted outside the 50.8-centimeter casing, within the 66.0-centimeter borehole for access to the Timber Mountain aquifer, and landed at 475.3 meters. A second string of 6.0-centimeter tubing with one slotted interval was inserted outside the 34.0-centimeter casing, within the 47.0-centimeter borehole for access to the Benham aquifer, and landed at 911.7 meters. A third piezometer string consists of 7.3-centimeter stainless-steel tubing that hangs from 6.0-centimeter carbon-steel tubing via a crossover sub. This string was landed at 1,029.5 meters to monitor the Tiva Canyon aquifer. The deepest string of 7.3-centimeter tubing was landed at 1,247.8 meters to monitor the Topopah Spring aquifer. Data collected during and shortly after hole construction include composite drill cuttings samples collected every 3.0 meters, 67 percussion gun and rotary sidewall core samples, various geophysical logs, fluid samples (for groundwater chemistry analysis and tritium measurements), and water-level measurements. The well penetrated 1,264.3 meters of Tertiary volcanic rock, including three saturated welded-tuff aquifers and one saturated lava-flow aquifer. A water level was measured in the Timber Mountain aquifer at 449.6 meters, during open-hole geophysical logging on September 20, 2009. The fluid level measured after the total depth was reached and the upper aquifer was cased off was 450.0 meters when measured in the open borehole on October 17, 2009. Measurements on samples taken from the undeveloped well indicated that tritium levels averaging approximately 12,430 picocuries per liter (less than Safe Drinking Water Act levels) were encountered within the Benham aquifer. Tritium was below the minimum detectable activity concentration for samples collected from the Tiva Canyon aquifer and the Topopah Spring aquifer.

  11. NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} core/shell nanocomposite: A highly efficient visible-light-driven photocatalyst utilizing upconversion

    SciTech Connect (OSTI)

    Sun, Yuanyuan; Wang, Wenzhong Sun, Songmei; Zhang, Ling

    2014-04-01

    Highlights: Design and synthesis of NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} based on upconversion. NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} nanocomposite was prepared for the first time. Coreshell structure benefits the properties. Upconversion contributed to the enhanced photocatalytic activity. Helps to understand the functionality of new type photocatalysts. - Abstract: NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} core/shell nanocomposite was designed and prepared for the first time based on upconversion. The products were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), high resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS) and diffuse reflectance spectra (DRS). The results revealed that the as-synthesized NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} consisted of spheres with a core diameter of about 26 nm and a shell diameter of around 6 nm. The core was upconversion illuminant NaYF{sub 4}:Er,Yb and the shell was Bi{sub 2}MoO{sub 6} around the core, which was confirmed by EDS. The NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} exhibited higher photocatalytic activity for the photodecomposition of Rhodamine B (RhB) under the irradiation of Xe lamp and green light emitting diode (g-LED). The mechanism of the high photocatalytic activity was discussed by photoluminescence spectra (PL), which is mainly attributed to upconversion of NaYF{sub 4}:Er,Yb in the NaYF{sub 4}:Er,Yb/Bi{sub 2}MoO{sub 6} nanocomposite and the coreshell structure.

  12. DOE/ER-0442

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2 Executive Summary: Atmospheric Radiation Measurement Program Plan ARM Program Plan ... The Atmospheric Radiation Measurement (ARM) Program was developed to supply an improved ...

  13. Pumping-route-dependent concentration quenching and temperature effect of green up- and down-conversion luminescence in Er{sup 3+}/Yb{sup 3+} co-doped Gd{sub 2}(WO{sub 4}){sub 3} phosphors

    SciTech Connect (OSTI)

    Li, Jingjing; Sun, Jiashi [Department of Physics, Dalian Maritime University, Dalian, Liaoning 116026 (China); Liu, Jutao [College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600 (China); Li, Xiangping; Zhang, Jinsu [Department of Physics, Dalian Maritime University, Dalian, Liaoning 116026 (China); Tian, Yue [Department of Physics, Dalian Maritime University, Dalian, Liaoning 116026 (China); College of Life Science, Dalian Nationalities University, Dalian, Liaoning 116600 (China); Fu, Shaobo; Cheng, Lihong; Zhong, Haiyang [Department of Physics, Dalian Maritime University, Dalian, Liaoning 116026 (China); Xia, Haiping [Key laboratory of Photo-electronic Materials, Ningbo University, Ningbo, Zhejiang 315211 (China); Chen, Baojiu, E-mail: chenmbj@sohu.com [Department of Physics, Dalian Maritime University, Dalian, Liaoning 116026 (China)

    2013-06-01

    Graphical abstract: A comparative study on the concentration quenching behaviors of green down- and up-conversion emissions was carried out for the first time, and the different concentration quenching mechanisms were analyzed. Secondly, the thermal effect induced by 980 nm LD irradiation was investigated, it was observed that the equilibrium temperature of Gd{sub 2}(WO{sub 4}){sub 3}:Er{sup 3+}/Yb{sup 3+} sample was decided by both the excitation power and Er{sup 3+} doping concentration. Highlights: ? Gd{sub 2}(WO{sub 4}){sub 3}:Er/Yb phosphors were prepared via a co-precipitation reaction. ? Morphology and structure of the phosphors were characterized by XRD and SEM. ? Concentration quenching mechanisms for down and up emissions were studied. ? Thermal effect induced by laser irradiation was studied via temperature sensing tech. - Abstract: Gd{sub 2}(WO{sub 4}){sub 3} phosphors with various Er{sup 3+} concentrations and fixed Yb{sup 3+} concentration were synthesized via a co-precipitation method, and their crystal structure and morphology were characterized by using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The concentration quenching behaviors of green up- and down-conversion emissions of Er{sup 3+} were analyzed, and it was confirmed that the difference between quenching concentration for up- and down-conversion emissions resulted from the different population routes. The temperature sensing properties of the Gd{sub 2}(WO{sub 4}){sub 3}:Er{sup 3+}/Yb{sup 3+} phosphors were studied, and it was found that the Er{sup 3+} doping concentration slightly affected the sensitivity, and Gd{sub 2}(WO{sub 4}){sub 3}:Er{sup 3+}/Yb{sup 3+} phosphors could be used in a broad temperature region for detecting temperature. Finally, the thermal effect induced by 980 nm LD irradiation was investigated, it was observed that the equilibrium temperature of Gd{sub 2}(WO{sub 4}){sub 3}:Er{sup 3+}/Yb{sup 3+} sample was decided by both the excitation power and Er{sup 3+} doping concentration.

  14. Well ER-6-1 Tracer Test Analysis: Yucca Flat, Nevada Test Site, Nye County, Nevada, Rev. No.: 0

    SciTech Connect (OSTI)

    Greg Ruskauff

    2006-09-01

    The ER-6-1 multiple-well aquifer test-tracer test (MWAT-TT) investigated groundwater flow and transport processes relevant to the transport of radionuclides from sources on the Nevada Test Site (NTS) through the lower carbonate aquifer (LCA) hydrostratigraphic unit (HSU). The LCA, which is present beneath much of the NTS, is the principal aquifer for much of southern Nevada. This aquifer consists mostly of limestone and dolomite, and is pervasively fractured. Groundwater flow in this aquifer is primarily in the fractures, and the hydraulic properties are primarily related to fracture frequency and fracture characteristics (e.g., mineral coatings, aperture, connectivity). The objective of the multiple-well aquifer test (MWAT) was to determine flow and hydraulic characteristics for the LCA in Yucca Flat. The data were used to derive representative flow model and parameter values for the LCA. The items of specific interest are: Hydraulic conductivity; Storage parameters; Dual-porosity behavior; and Fracture flow characteristics. The objective of the tracer transport experiment was to evaluate the transport properties and processes of the LCA and to derive representative transport parameter values for the LCA. The properties of specific interest are: Effective porosity; Matrix diffusion; Longitudinal dispersivity; Adsorption characteristics; and Colloid transport characteristics. These properties substantially control the rate of transport of contaminants in the groundwater system and concentration distributions. To best support modeling at the scale of the corrective action unit (CAU), these properties must be investigated at the field scale. The processes represented by these parameters are affected by in-situ factors that are either difficult to investigate at the laboratory scale or operate at a much larger scale than can be reproduced in the laboratory. Measurements at the field scale provide a better understanding of the effective average parameter values. The scale of this tracer test is still small compared to the scale of a CAU, but is of sufficient scale to be generally representative of the processes that affect in-situ transport. The scale of the tracer test undertaken is limited by the rate of transport in the formation and the resultant time frame required for completing such a test. The measurements at the field scale will provide information for relating laboratory measurements for transport processes to the larger scale. This report describes the analysis of the tracer test data and development of a conceptual model of transport in the LCA in Yucca Flat.

  15. As you may kn&<' the~de&tment of &~er& (D&j 1s involved'in'a pronram

    Office of Legacy Management (LM)

    As you may kn&<' the~de&tment of &~er& (D&j 1s involved'in'a pronram '. to'chiiracterlze the radjologital cbndif~on of ,sites formerly used byythe . . . ., Manhattan Engineer Dlstrlct (NED) and/or Atomjc Energy Co$n~~lssiqq (AEC); in.. the development of 'nuclear energy.. As part..of this -programi' DOE is 1~ I+ preparing, ,a' series of. brJef~ summaries ,-of .the' history:. of' tho ,#D/AEC~ : : ..; 'i ..relatecl activities and 'Conditions at .thc. sneclfic. sites. The

  16. Phytoestrogens in menopausal supplements induce ER-dependent cell proliferation and overcome breast cancer treatment in an in vitro breast cancer model

    SciTech Connect (OSTI)

    Duursen, Majorie B.M. van; Smeets, Evelien E.J.W.; Rijk, Jeroen C.W.; Nijmeijer, Sandra M.; Berg, Martin van den

    2013-06-01

    Breast cancer treatment by the aromatase inhibitor Letrozole (LET) or Selective Estrogen Receptor Modulator Tamoxifen (TAM) can result in the onset of menopausal symptoms. Women often try to relieve these symptoms by taking menopausal supplements containing high levels of phytoestrogens. However, little is known about the potential interaction between these supplements and breast cancer treatment, especially aromatase inhibitors. In this study, interaction of phytoestrogens with the estrogen receptor alpha and TAM action was determined in an ER-reporter gene assay (BG1Luc4E2 cells) and human breast epithelial tumor cells (MCF-7). Potential interactions with aromatase activity and LET were determined in human adrenocorticocarcinoma H295R cells. We also used the previously described H295R/MCF-7 co-culture model to study interactions with steroidogenesis and tumor cell proliferation. In this model, genistein (GEN), 8-prenylnaringenin (8PN) and four commercially available menopausal supplements all induced ER-dependent tumor cell proliferation, which could not be prevented by physiologically relevant LET and 4OH-TAM concentrations. Differences in relative effect potencies between the H295R/MCF-7 co-culture model and ER-activation in BG1Luc4E2 cells, were due to the effects of the phytoestrogens on steroidogenesis. All tested supplements and GEN induced aromatase activity, while 8PN was a strong aromatase inhibitor. Steroidogenic profiles upon GEN and 8PN exposure indicated a strong inhibitory effect on steroidogenesis in H295R cells and H295R/MCF-7 co-cultures. Based on our in vitro data we suggest that menopausal supplement intake during breast cancer treatment should better be avoided, at least until more certainty regarding the safety of supplemental use in breast cancer patients can be provided. - Highlights: Supplements containing phytoestrogens are commonly used by women with breast cancer. Phytoestrogens alter steroidogenesis in a co-culture breast cancer model. Letrozole or tamoxifen treatment is used to inhibit ER-dependent breast tumor growth. Phytoestrogens induce in vitro tumor cell growth, even in combination with LET or TAM. Use of phytoestrogens during breast cancer treatment should better be avoided.

  17. Preparation and thermophysical properties of (Sm{sub 1?x}Er{sub x}){sub 2}Ce{sub 2}O{sub 7} oxides for thermal barrier coatings

    SciTech Connect (OSTI)

    Xiaoge, Chen; Shusen, Yang; Hongsong, Zhang; Gang, Li; Zhenjun, Li.; Bo, Ren; Xudan, Dang; Haoming, Zhang; An, Tang

    2014-03-01

    Graphical abstract: - Highlights: These ceramic materials with fluorite structure were synthesized. Defect points lead to their lower thermal conductivities. The lower ionic radius of Er{sup 3+} ion leads to the reduction of thermal expansion coefficient of (Sm{sub 1?x}Er{sub x}){sub 2}Ce{sub 2}O{sub 7} oxides. - Abstract: (Sm{sub 1?x}Er{sub x}){sub 2}Ce{sub 2}O{sub 7} ceramics were synthesized by solgel method and sintered at 1600 C for 10 h in air. The influence of Er{sub 2}O{sub 3}-substitution on the phase structure and thermophysical properties of Sm{sub 2}Ce{sub 2}O{sub 7} was investigated. The phase structures of these ceramics were identified by X-ray diffraction showing that all synthesized ceramics have fluorite-type structure. The measurements for thermophysical properties of these ceramics show that their thermal conductivities and thermal expansion coefficients remarkably decreased through Er-substitution. However, the thermal expansion coefficients were higher than that of YSZ and their thermal conductivities were much lower than that of 8YSZ. The excellent thermophysical property implies that these solid solutions are potential materials for the ceramics layer in thermal barrier coatings.

  18. The breakdown of de Gennes Scaling in TbxEr1-xNi2B2C and its mean field theory explanation

    SciTech Connect (OSTI)

    Gao, Chunwang

    2005-05-01

    The Neel temperatures, T{sub N}, of Tb{sub x}Er{sub 1-x}Ni{sub 2}B{sub 2}C samples have been determined from the temperature dependence of magnetization measurements. A breakdown of the de Gennes scaling of T{sub N} with a clear turning point around x = 0.45 has been observed. The T{sub N} values of Tb{sub x}Er{sub 1-x}Ni{sub 2}B{sub 2}C do not change much within the range of O < x < 0.45 and then, for larger x they increase almost linearly with concentration until T{sub N} = 14.9K is reached for x = 1, TbNi{sub 2}B{sub 2}C. The clear change in the x-dependence of T{sub N} around x = 0.45 can be linked to a change in the local moment ordering direction from transverse to longitudinal, a change which is consistent with recent resonant X-ray scattering data. These features in T{sub N}(x) can be explained using a mean field model.

  19. Effect of Mn doping on structural and magnetic susceptibility of C-type rare earth nano oxides Er{sub 2?x}Mn{sub x}O{sub 3}

    SciTech Connect (OSTI)

    Heiba, Zein K.; Taif University, Faculty of Science, Physics Department ; Mohamed, Mohamed Bakr; Fuess, H.

    2012-12-15

    Graphical abstract: Display Omitted Highlights: ? Er{sub 2?x}Mn{sub x}O{sub 3} (0.0 ? x ? 0.20) prepared by solgel method. ? The change in lattice parameter is not linear with x due to the change in crystallite size with doping. ? Anomalous concentration dependence is found in magnetic susceptibility. ? The effective magnetic moment ?{sub eff} is found to decrease with composition parameter x. ? Superexchange interactions between Er ions depending on the amount of Mn or Er in different sites. -- Abstract: The manganese doped rare earth oxides Er{sub 2?x}Mn{sub x} O{sub 3} (0.0 ? x ? 0.20) were synthesized by a solgel process and analyzed by X-ray diffraction using Rietveld refinement methods. A single phase solid solution is formed up to x = 0.15 while for x ? 0.2 a manganese oxide phase appears in the diffraction pattern. Preferential cationic distribution between the non-equivalent sites 8b and 24d of space group Ia3{sup } is found for all samples but to a different extent. The octahedral volume and average bond length of Er{sub 1}-O for 8b site decrease while both octahedral volume and bond length of Er{sub 2}-O for 24d site increase. Magnetization measurements were done in the temperature range 5300 K. The effective magnetic moment ?{sub eff} is found to decrease with composition parameter x, except for sample x = 0.05 where the magnetization is enhanced. The Curie-Weiss paramagnetic temperatures indicate antiferromagnetic interaction.

  20. Laser-induced magnetic fields in ICF capsules, Final Report, DE-FG02-08ER85128, Phase 1

    SciTech Connect (OSTI)

    Lindman, Erick L

    2009-11-05

    Laser-induced magnetic fields in ICF capsules Final Report, DE-FG02-08ER85128, Phase 1 E. L. LINDMAN, Otowi Technical Services, Los Alamos, NM. The performance of an inertial-confinement-fusion (ICF) capsule can be improved by inserting a magnetic field into it before compressing it [Kirkpatrick, et al., Fusion Technol. 27, 205 (1995)]. To obtain standoff in an ICF power generator, a method of inserting the field without the use of low-inductance leads attached to the capsule is desired. A mechanism for generating such a field using a laser was discovered in Japan [Sakagami, et al., Phys. Rev. Lett. 42, 839 (1979), Kolodner and Yablonovitch, Phys. Rev. Lett. 43, 1402 (1979)] and studied at Los Alamos in the 1980s [M. A. Yates, et al., Phys. Rev. Lett. 49, 1702 (1982); Forslund and Brackbill, Phys. Rev. Lett. 48, 1614 (1982)]. In this mechanism, a p-polarized laser beam strikes a solid target producing hot electrons that are accelerated away from the target surface by resonant absorption. An electric field is created that returns the hot electrons to the target. But, they do not return to the target along the same trajectory on which they left. The resulting current produces a toroidal magnetic field that was observed to spread over a region outside the hot spot with a radius of a millimeter. No experimental measurements of the magnetic field strength were performed. Estimates from computer simulation suggest that field strengths in the range of 1 to 10 Mega gauss (100 to 1000 Tesla) were obtained outside of the laser spot. To use this mechanism to insert a magnetic field into an ICF capsule, the capsule must be redesigned. In one approach, a central conductor is added, a toroidal gap is cut in the outer wall and the DT fuel is frozen on the inner surface of the capsule. The capsule is dropped into the reaction chamber and struck first with the laser that generates the magnetic field. The laser hot spot is positioned at the center of the toroidal gap. As the magnetic field spreads from the hot spot over the surface that contains the toroidal gap, it will propagate through the gap and set up a steady state in the capsule. The main compression is then initiated. First, it closes the gap and crow-bars the field, then it compresses the fuel to ignition. In addition to this application, we discuss the use of this mechanism to induce Mega-gauss fields in laboratory apparatus for measurements of the effects of large magnetic fields on material samples. A preliminary target design for this purpose is presented. It is made of high-density material with no hydrogen surface contamination to minimize fast ion losses and to minimize x-ray preheat of the sample (the material, whose magnetic properties are to be measured). In it, the gap is designed to allow the magnetic field to move into the interior of the target while minimizing the flow of hot electrons into the interior. By adjusting the size of the gap as well as its configuration, the hot electron effects can be minimized. Since the strength of the magnetic field depends on the radial distance to the sample from the center of the conductor carrying the return current, the sample is located at a point of minimum conductor radius. This location also minimizes the effects on the measurement of any hot electrons that flow into the interior. Useful experiments can be accomplished with the seed field alone in this geometry. Compressing the capsule after the insertion of the seed field may allow experiments with even larger magnetic fields. We have used computer-simulation techniques to address a number of issues. Our conclusions include: This magnetic-field generating mechanism is a viable method for generating magnetic fields in ICF targets and for laboratory experiments. Useful experiments on material samples can be done with the seed field of 1 to10 Mega gauss (100 to 1000 Tesla) and higher magnetic fields can be obtained by subsequently compressing the capsule. The results reported here can be studied experimentally with a modest CO2 laser that emits 4.5 J of ene

  1. Negative to positive magnetoresistance and magnetocaloric effect in Pr0.6Er0.4Al2

    SciTech Connect (OSTI)

    Pathak, Arjun K.; Gschneidner, Jr., K. A.; Pecharsky, V. K.

    2014-10-13

    We report on the magnetic, magnetocaloric and magnetotransport properties of Pr0.6Er0.4Al2. The title compound exhibits a large positive magnetoresistance (MR) for H ≥ 40 kOe and a small but non negligible negative MR for H ≤ 30 kOe. The maximum positive MR reaches 13% at H = 80 kOe. The magnetic entropy and adiabatic temperature changes as functions of temperature each show two anomalies: a broad dome-like maximum below 20 K and a relatively sharp peak at higher temperature. As a result, observed behaviors are unique among other binary and mixed lanthanide compounds.

  2. High-resolution study of 0{sup +} and 2{sup +} excitations in {sup 168}Er with the (p,t) reaction

    SciTech Connect (OSTI)

    Bucurescu, D. [Horia Hulubei National Institute of Physics and Nuclear Engineering, P.O. Box MG-6, R-76900 Bucharest (Romania); Graw, G.; Hertenberger, R. [Sektion Physik, Ludwig Maximilians Universitaet Muenchen, Am Coulombwall 1, D-85748 Garching (Germany); Wirth, H.-F.; Faestermann, T.; Kruecken, R.; Mahgoub, M. [Physik Department, Technische Universitaet Muenchen, D-85748 Garching (Germany); Lo Iudice, N. [Dipartimento di Scienze Fisiche, Universita di Napoli 'Federico II' and Istituto Nazionale di Fisica Nucleare, Monte S Angelo, Via Cintia I-80126 Naples (Italy); Sushkov, A.V.; Shirikova, N.Yu. [Bogoliubov Laboratory of Theoretical Physics, Joint Institute for Nuclear Research, RU-141980 Dubna (Russian Federation); Sun, Y. [Department of Physics and Joint Institute for Nuclear Astrophysics, University of Notre Dame, Notre Dame, Indiana 46556 (United States); Department of Physics, Tsinghua University, Beijing 100084 (China); Department of Physics, Xuzhou Normal University, Xuzhou, Jiangsu 221009 (China); Jolie, J.; Brentano, P. von; Braun, N.; Heinze, S.; Moeller, O.; Muecher, D.; Scholl, C. [Institut fuer Kernphysik, Universitaet zu Koeln, D-50937 Cologne (Germany); Casten, R.F.; Meyer, D.A. [Wright Nuclear Structure Laboratory, Yale University, New Haven, Connecticut 06520-8124 (United States)

    2006-06-15

    Excited states in the deformed nucleus {sup 168}Er have been studied with high-energy resolution, in the (p,t) reaction, with the Munich Q3D spectrograph. A number of 25 excited 0{sup +} states (four tentative) and 63 2{sup +} states have been assigned up to 4.0 MeV excitation energy. This unusually rich characterization of the 0{sup +} and 2{sup +} states in a deformed nucleus, close to a complete level scheme, offers a unique opportunity to check, in detail, models of nuclear structure that incorporate many excitation modes. A comparison of the experimental data is made with two such models: the quasiparticle-phonon model (QPM), and the projected shell model (PSM). The PSM wave functions appear to contain fewer correlations than those of the QPM and than required by the data.

  3. Analysis of In situ Observations of Cloud Microphysics from M-PACE Final Report, DOE Grant Agreement No. DE-FG02-06ER64168

    SciTech Connect (OSTI)

    Michael R. Poellot

    2009-01-09

    This report summarizes the findings and accomplishments of work performed under DOE Grant Agreement No. DE-FG02-06ER64168. The focus of the work was the analysis of in situ observations collected by the University of North Dakota Citation research aircraft during the Mixed-Phase Arctic Cloud Experiment (M-PACE). This project was conducted in 2004 along the North Slope of Alaska. The objectives of the research were: to characterize certain microphysical properties of clouds sampled during M-PACE, including spatial variability, precipitation formation, ice multiplication; to examine instrument performance and certain data processing algorithms; and to collaborate with other M-PACE investigators on case study analyses. A summary of the findings of the first two objectives is given here in parts 1 and 2; full results are contained in reports listed in part 3 of this report. The collaborative efforts are described in the publications listed in part 3.

  4. Rare-earth-rich tellurides: Gd{sub 4}NiTe{sub 2} and Er{sub 5}M{sub 2}Te{sub 2} (M=Co, Ni)

    SciTech Connect (OSTI)

    Magliocchi, Carmela; Meng, Fanqin; Hughbanks, Timothy . E-mail: trh@mail.chem.tamu.edu

    2004-11-01

    Three new rare earth metal-rich compounds, Gd{sub 4}NiTe{sub 2}, and Er{sub 5}M{sub 2}Te{sub 2} (M=Ni, Co), were synthesized in direct reactions using R, R{sub 3}M, and R{sub 2}Te{sub 3} (R=Gd, Er; M=Co, Ni) and single-crystal structures were determined. Gd{sub 4}NiTe{sub 2} is orthorhombic and crystallizes in space group Pnma with four formula units per cell. Lattice parameters at 110(2)K are a=15.548(9), b=4.113(2), c=11.7521(15)A. Er{sub 5}Ni{sub 2}Te{sub 2} and Er{sub 5}Co{sub 2}Te{sub 2} are isostructural and crystallize in the orthorhombic space group Cmcm with two formula units per cell. Lattice parameters at 110(2)K are a=3.934(1), b=14.811(4), c=14.709(4)A, and a=3.898(1), b=14.920(3), c=14.889(3)A, respectively. Metal-metal bonding correlations were analyzed using the empirical Pauling bond order concept.

  5. Sexually dimorphic gene regulation in brain as a target for endocrine disrupters: Developmental exposure of rats to 4-methylbenzylidene camphor

    SciTech Connect (OSTI)

    Maerkel, Kirsten [Institute of Pharmacology and Toxicology and GREEN Tox, University of Zurich, CH-8057 Zurich (Switzerland); Durrer, Stefan [Institute of Pharmacology and Toxicology and GREEN Tox, University of Zurich, CH-8057 Zurich (Switzerland); Henseler, Manuel [Institute of Pharmacology and Toxicology and GREEN Tox, University of Zurich, CH-8057 Zurich (Switzerland); Schlumpf, Margret [Institute of Pharmacology and Toxicology and GREEN Tox, University of Zurich, CH-8057 Zurich (Switzerland); Lichtensteiger, Walter [Institute of Pharmacology and Toxicology and GREEN Tox, University of Zurich, CH-8057 Zurich (Switzerland)]. E-mail: Walter.Lichtensteiger@access.unizh.ch

    2007-01-15

    The developing neuroendocrine brain represents a potential target for endocrine active chemicals. The UV filter 4-methylbenzylidene camphor (4-MBC) exhibits estrogenic activity, but also interferes with the thyroid axis. We investigated effects of pre- and postnatal exposure to 4-MBC in the same rat offspring at brain and reproductive organ levels. 4-MBC (7, 24, 47 mg/kg/day) was administered in chow to the parent generation before mating, during gestation and lactation, and to the offspring until adulthood. mRNA of estrogen target genes involved in control of sexual behavior and gonadal functions was measured by real-time RT-PCR in ventromedial hypothalamic nucleus (VMH) and medial preoptic area (MPO) of adult offspring. 4-MBC exposure affected mRNA levels of ER alpha, progesterone receptor (PR), preproenkephalin (PPE) and insulin-like growth factor-I (IGF-I) in a sex- and region-specific manner. In order to assess possible changes in sensitivity of target genes to estrogens, offspring were gonadectomized on day 70, injected with estradiol (E2, 10 or 50 {mu}g/kg s.c.) or vehicle on day 84, and sacrificed 6 h later. The acute induction of PR mRNA, and repression (at 6 h) of PPE mRNA by E2 was enhanced by 4-MBC in male and female VMH and female MPO, whereas male MPO exhibited reduced responsiveness of both genes. Steroid receptor coactivator SRC-1 mRNA levels were increased in female VMH and MPO. The data indicate profound sex- and region-specific alterations in the regulation of estrogen target genes at brain level. Effect patterns in baseline and E2-induced gene expression differ from those in uterus and prostate.

  6. Genetic maps of polymorphic DNA loci on rat chromosome 1

    SciTech Connect (OSTI)

    Ding, Yan-Ping; Remmers, E.F.; Longman, R.E.

    1996-09-01

    Genetic linkage maps of loci defined by polymorphic DNA markers on rat chromosome 1 were constructed by genotyping F2 progeny of F344/N x LEW/N, BN/SsN x LEW/N, and DA/Bkl x F344/Hsd inbred rat strains. In total, 43 markers were mapped, of which 3 were restriction fragment length polymorphisms and the others were simple sequence length polymorphisms. Nineteen of these markers were associated with genes. Six markers for five genes, {gamma}-aminobutyric acid receptor {beta}3 (Gabrb3), syntaxin 2 (Stx2), adrenergic receptor {beta}3 (Gabrb3), syntaxin 2 (Stx2), adrenergic receptor {beta}1 (Adrb1), carcinoembryonic antigen gene family member 1 (Cgm1), and lipogenic protein S14 (Lpgp), and 20 anonymous loci were not previously reported. Thirteen gene loci (Myl2, Aldoa, Tnt, Igf2, Prkcg, Cgm4, Calm3, Cgm3, Psbp1, Sa, Hbb, Ins1, and Tcp1) were previously mapped. Comparative mapping analysis indicated that the large portion of rat chromosome 1 is homologous to mouse chromosome 7, although the homologous to mouse chromosome 7, although the homologs of two rat genes are located on mouse chromosomes 17 and 19. Homologs of the rat chromosome 1 genes that we mapped are located on human chromosomes 6, 10, 11, 12, 15, 16, and 19. 38 refs., 1 fig., 3 tabs.

  7. Inhalation developmental toxicology studies: Gallium arsenide in mice and rats

    SciTech Connect (OSTI)

    Mast, T.J.; Greenspan, B.J.; Dill, J.A.; Stoney, K.H.; Evanoff, J.J.; Rommereim, R.L.

    1990-12-01

    Gallium arsenide is a crystalline compound used extensively in the semiconductor industry. Workers preparing solar cells and gallium arsenide ingots and wafers are potentially at risk from the inhalation of gallium arsenide dust. The potential for gallium arsenide to cause developmental toxicity was assessed in Sprague- Dawley rats and CD-1 (Swiss) mice exposed to 0, 10, 37, or 75 mg/m{sup 3} gallium arsenide, 6 h/day, 7 days/week. Each of the four treatment groups consisted of 10 virgin females (for comparison), and {approx}30 positively mated rats or {approx}24 positively mated mice. Mice were exposed on 4--17 days of gestation (dg), and rats on 4--19 dg. The day of plug or sperm detection was designated as 0 dg. Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice (rats, 20 dg; mice, 18 dg). Implants were enumerated and their status recorded. Live fetuses were sexed and examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. Gallium and arsenic concentrations were determined in the maternal blood and uterine contents of the rats (3/group) at 7, 14, and 20 dg. 37 refs., 11 figs., 30 tabs.

  8. Inhalation developmental toxicology studies: Acetonitrile in rats. Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Weigel, R.J.; Westerberg, R.B.; Boyd, P.J.; Hayden, B.K.; Evanoff, J.J.; Rommereim, R.L.

    1994-02-01

    The potential for acetonitrile to cause developmental toxicity was assessed in Sprague-Dawley rats exposed to 0, 100, 400, or 1200 ppM acetonitrile, 6 hours/day, 7 days/week. Exposure of rats to these concentrations of acetonitrile resulted in mortality in the 1200 ppM group (2/33 pregnant females; 1/10 non-pregnant females). However, there were no treatment-related effects upon body weights or reproduction indices at any exposure level, nor was there a significant increase in the incidence of fetal malformations or variations. The only effect observed in the fetuses was a slight, but not statiscally significant, exposure-correlated increase in the incidence of supernumerary ribs. Determination of acetonitrile and cyanide concentrations in maternal rat blood showed that acetonitrile concentration in the blood increased with exposure concentration for all exposed maternal rats. Detectable amounts of cyanide in the blood were found only in the rats exposed to 1200 ppM acetonitrile ({approximately}2 {mu}g cyanide/g of blood).

  9. Radiation leukaemogenesis at low doses DE-FG02-05 ER 63947 Final Technical Report 15 May 2005 ?? 14 May 2010

    SciTech Connect (OSTI)

    Simon Bouffler; Christophe Badie; Natalie Brown; Rosemary Finnon

    2010-07-28

    This report provides a full summary of the results obtained under grant DE-FG02-05ER63947, Radiation Leukaemogenesis at low doses. The studies employed an experimental model of radiation leukaemogenesis with the main aim of identifying key events that convert normal cells into leukaemic cells follwoing exposure to radiation. Important aspect of the work was to understand dose-response relationships and time course relationships for leakaemogenis events. The studies performed provided evidence for direct radiation-induced losses of the Sfpi1/PU.1 gene being critical for induction of the disease. No threshold below 0.1 Gy in the induction of the gene losses was observed. The critical cell type in which the myeloid lekaemias arise has been identified and point mutations in the Sfpi1/PU.1 gene are common in leukaemias. The consequences of the genetic losses and mutation have been examined and these provide evidence of a disruption of differentiation in leukaemic cells. Additional pathways to leukaemogenesis have been identified also. Overall the study provides quantitiative data suitable for testing approaches to modelling of leukaemia rosk at low doses.

  10. Thermoelectric power generator module of 16x16 Bi{sub 2}Te{sub 3} and 0.6%ErAs:(InGaAs){sub 1-x}(InAlAs){sub x} segmented elements

    SciTech Connect (OSTI)

    Zeng Gehong; Bahk, Je-Hyeong; Bowers, John E.; Lu Hong; Gossard, Arthur C.; Singer, Suzanne L.; Majumdar, Arun; Bian, Zhixi; Zebarjadi, Mona; Shakouri, Ali

    2009-08-24

    We report the fabrication and characterization of thermoelectric power generator modules of 16x16 segmented elements consisting of 0.8 mm thick Bi{sub 2}Te{sub 3} and 50 {mu}m thick ErAs:(InGaAs){sub 1-x}(InAlAs){sub x} with 0.6% ErAs by volume. An output power up to 6.3 W was measured when the heat source temperature was at 610 K. The thermoelectric properties of (InGaAs){sub 1-x}(InAlAs){sub x} were characterized from 300 up to 830 K. The finite element modeling shows that the performance of the generator modules can further be enhanced by improving the thermoelectric properties of the element materials, and reducing the electrical and thermal parasitic losses.

  11. Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy

    SciTech Connect (OSTI)

    Wozei, E.; Hermanowicz, S.W.; Holman, H-Y.N.

    2006-01-01

    Using a fluorescein di-{beta}-d-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17{beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 min of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification methods.

  12. Developing a Biosensor for Estrogens in Water Samples: Study ofthe Real-time Response of Live Cells of the Estrogen-sensitive YeastStrain RMY/ER-ERE using Fluorescence Microscopy

    SciTech Connect (OSTI)

    Wozei, E.; Hermanowicz, S.W.; Holman, H-Y.N.

    2005-07-13

    Using a fluorescein di-{beta}-D-galactopyranoside (FDG) substrate we show that in live cells of an estrogen-sensitive yeast strain RMY/ER-ERE with human estrogen receptor (ER{alpha}) gene and the lacZ gene which encodes {beta}-galactosidase, the uptake of 17 {beta}-estradiol (E2) and the subsequent production of {beta}-galactosidase enzyme occur quite rapidly, with maximal enzyme-catalyzed product formation evident after about 30 minutes of exposure to E2. This finding which agrees with the well-known rates of enzyme-catalyzed reactions could have implications for shortening the duration of environmental sample screening and monitoring regimes using yeast-based estrogen assays, and the development of biosensors for environmental estrogens to complement quantification methods.

  13. Fine tunable red-green upconversion luminescence from glass ceramic containing 5%Er{sup 3+}:NaYF{sub 4} nanocrystals under excitation of two near infrared femtosecond lasers

    SciTech Connect (OSTI)

    Shang, Xiaoying; Cheng, Wenjing; Zhou, Kan; Ma, Jing; Feng, Donghai; Zhang, Shian; Sun, Zhenrong; Jia, Tianqing; Chen, Ping; Qiu, Jianrong

    2014-08-14

    In this paper, we report fine tunable red-green upconversion luminescence of glass ceramic containing 5%Er{sup 3+}: NaYF{sub 4} nanocrystals excited simultaneously by two near infrared femtosecond lasers. When the glass ceramic was irradiated by 800?nm femtosecond laser, weak red emission centered at 670?nm was detected. Bright red light was observed when the fs laser wavelength was tuned to 1490?nm. However, when excited by the two fs lasers simultaneously, the sample emitted bright green light centered at 550?nm, while the red light kept the same intensity. The dependences of the red and the green light intensities on the two pump lasers are much different, which enables us to manipulate the color emission by adjusting the two pump laser intensities, respectively. We present a theoretical model of Er{sup 3+} ions interacting with two fs laser fields, and explain well the experimental results.

  14. Magnetic properties and magnetocaloric effect in the RCu{sub 2}Si{sub 2} and RCu{sub 2}Ge{sub 2} (R = Ho, Er) compounds

    SciTech Connect (OSTI)

    Mo, Zhao-Jun; Shen, Jun E-mail: tangcc@hebut.edu.cn; Wu, Jian-Feng; Yan, Li-Qin; Wang, Li-Chen; Sun, Ji-rong; Shen, Bao-Gen; Gao, Xin-Qiang; Tang, Cheng-Chun E-mail: tangcc@hebut.edu.cn

    2014-02-21

    The magnetic properties and magnetocaloric effect (MCE) in RCu{sub 2}Si{sub 2} and RCu{sub 2}Ge{sub 2} (R = Ho, Er) compounds have been investigated. All these compounds possess an antiferromagnetic (AFM)-paramagnetic (PM) transition around their respective Neel temperatures. The RCu{sub 2}Si{sub 2} compounds undergo spin-glassy behavior above Neel temperature. Furthermore, a field-induced metamagnetic transition from AFM to ferromagnetic (FM) states is observed in these compounds. The calculated magnetic entropy changes show that all RCu{sub 2}Si{sub 2} and RCu{sub 2}Ge{sub 2} (R = Ho, Er) compounds, especially, ErCu{sub 2}Si{sub 2} exhibits large MCEs with no thermal hysteresis and magnetic hysteresis loss. The value of −ΔS{sub M}{sup max} reaches 22.8 J/Kg K for magnetic field changes from 0 to 5 T. In particular, for field changes of 1 and 2 T, the giant reversible magnetic entropy changes −ΔS{sub M}{sup max} are 8.3 and 15.8 J/kg K at 2.5 K, which is lower than the boiling point of helium. The low-field giant magnetic entropy change, together with ignorable thermal hysteresis and field hysteresis loss of ErCu{sub 2}Si{sub 2} compound is expected to have effective applications in low temperature magnetic refrigeration.

  15. Analysis of Hydraulic Responses from the ER-6-1 Multiple-Well Aquifer Test, Yucca Flat FY 2004 Testing Program, Nevada Test Site, Nye County, Nevada, Rev. No.: 0

    SciTech Connect (OSTI)

    Greg Ruskauff

    2005-06-01

    This report documents the interpretation and analysis of the hydraulic data collected for the Fiscal Year (FY) 2004 Multiple-Well Aquifer Test-Tracer Test (MWAT-TT) conducted at the ER-6-1 Well Cluster in Yucca Flat Corrective Action Unit (CAU) 97, on the Nevada Test Site (NTS). The MWAT-TT was performed to investigate CAU-scale groundwater flow and transport processes related to the transport of radionuclides from sources on the NTS through the Lower Carbonate Aquifer (LCA) Hydrostratigraphic Unit (HSU). The ER-6-1 MWAT-TT was planned and executed by contractor participants for the Underground Test Area (UGTA) Project of the Environmental Restoration (ER) program of the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Site Office (NNSA/NSO). Participants included Stoller-Navarro Joint Venture (SNJV), the Environmental Engineering Services Contractor; Bechtel Nevada (BN); the Desert Research Institute (DRI); Los Alamos National Laboratory; and the University of Nevada, Las Vegas-Harry Reid Center. The SNJV team consists of the S.M. Stoller Corporation, Navarro Research and Engineering, Battelle Memorial Institute, INTERA Inc., and Weston Solutions, Inc. The MWAT-TT was implemented according to the ''Underground Test Area Project, ER-6-1 Multi-Well Aquifer Test - Tracer Test Plan'' (SNJV, 2004a) issued in April 2004. The objective of the aquifer test was to determine flow processes and local hydraulic properties for the LCA through long-term constant-rate pumping at the well cluster. This objective was to be achieved in conjunction with detailed sampling of the composite tracer breakthrough at the pumping well, as well as with depth-specific sampling and logging at multiple wells, to provide information for the depth-discrete analysis of formation hydraulic properties, particularly with regard to fracture properties.

  16. Bifunction in Er{sup 3+}/Yb{sup 3+} co-doped BaTi{sub 2}O{sub 5}Gd{sub 2}O{sub 3} glasses prepared by aerodynamic levitation method

    SciTech Connect (OSTI)

    Zhang, Minghui; Yu, Jianding; Pan, Xiuhong; Cheng, Yuxing; Liu, Yan

    2013-11-15

    Graphical abstract: - Highlights: Novel BaTi{sub 2}O{sub 5}Gd{sub 2}O{sub 3} based glasses have been prepared by aerodynamic levitation. The obtained glasses show high thermal stability with T{sub g} = 763.3 C. Er{sup 3+}/Yb{sup 3+} co-doped glasses show strong upconversion based on a two-photon process. Red emission is stronger than green emissions for EBT by high Yb{sup 3+} concentration. Magnetic ions are paramagnetic and the distribution is homogeneous in the glasses. - Abstract: Novel Er{sup 3+}/Yb{sup 3+} co-doped BaTi{sub 2}O{sub 5}Gd{sub 2}O{sub 3} spherical glasses have been fabricated by aerodynamic levitation method. The thermal stability, upconversion luminescence, and magnetic properties of the present glass have been studied. The glasses show high thermal stability with 763.3 C of the onset temperature of the glass transition. Red and green emissions centered at 671 nm, 548 nm and 535 nm are obtained at 980 nm excitation. The upconversion is based on a two-photon process by energy transfer, excited-state absorption, and energy back transfer. Yb{sup 3+} ions are more than Er{sup 3+} ions in the glass, resulting in efficient energy back transfer from Er{sup 3+} to Yb{sup 3+}. So the red emission is stronger than the green emissions. Magnetization curves indicate that magnetic rare earth ions are paramagnetic and the distribution is homogeneous and random in the glass matrix. Aerodynamic levitation method is an efficient way to prepare glasses with homogeneous rare earth ions.

  17. Final report (Grant No. DOE DE-FG02-97ER62366) [Retrieval of cloud fraction and type using broadband diffuse and total shortwave irradiance measurements

    SciTech Connect (OSTI)

    Clothiaux, Eugene

    2001-05-17

    The primary research effort supported by Grant No. DOE DEFG02-97ER62366 titled ''Retrieval of Cloud Fraction and Type Using Broadband Diffuse and Total Shortwave Irradiance Measurements'' was application of clear-sky identification and cloud fraction estimation algorithms developed by Charles N. Long and Thomas P. Ackerman to the downwelling total, direct and diffuse shortwave irradiance measurements made at all of the central, boundary, and extended facilities of the DOE Atmospheric Radiation Measurement (ARM) Program Southern Great Plains (SOP) site. Goals of the research were finalization and publication of the two algorithms in the peer-reviewed literature and operational application of them to all of aforementioned data streams from the ARM SGP site. The clear-sky identification algorithm was published as Long and Ackerman (2000) in the Journal of Geophysical Research, while a description of the cloud fraction estimation algorithm made it to the scientific literature as Long et al. (1999) in the Proceedings of the 10th American Meteorological Association Conference on Atmospheric Radiation held in Madison, Wisconsin. The cloud fraction estimation algorithm relies on empirical relationships between the outputs of the clear-sky identification algorithm and cloud fraction; as such, the cloud fraction estimation algorithm requires significant amounts of data both to properly develop the empirical relationships and to thoroughly test them. With this perspective in mind the major focus of our research efforts in the later half of the project became the operational implementation of the clear-sky identification algorithm on DOE ARM SGP data so that we could develop the data set necessary for final tuning of the cloud fraction estimation algorithm in research extending beyond the lifetime of the project.

  18. miller-er-99.PDF

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Correction for Dry Bias in Vaisala Radiosonde RH Data E. R. Miller, J. Wang, and H. L. Cole National Center for Atmospheric Research Atmospheric Technology Division Boulder, Colorado Abstract Extensive data analysis of sounding data from the Tropical Ocean Global Atmosphere-Coupled Ocean Atmosphere Response Experiment (TOGA-COARE) and other research projects coupled with supporting evidence from other sources have lead to the conclusion that there is a dry bias in Vaisala radiosonde relative

  19. Final Report for DOE Grant DE-FG02-03ER25579; Development of High-Order Accurate Interface Tracking Algorithms and Improved Constitutive Models for Problems in Continuum Mechanics with Applications to Jetting

    SciTech Connect (OSTI)

    Puckett, Elbridge Gerry; Miller, Gregory Hale

    2012-10-14

    Much of the work conducted under the auspices of DE-FG02-03ER25579 was characterized by an exceptionally close collaboration with researchers at the Lawrence Berkeley National Laboratory (LBNL). For example, Andy Nonaka, one of Professor Miller's graduate students in the Department of Applied Science at U. C. Davis (UCD) wrote his PhD thesis in an area of interest to researchers in the Applied Numerical Algorithms Group (ANAG), which is a part of the National Energy Research Supercomputer Center (NERSC) at LBNL. Dr. Nonaka collaborated closely with these researchers and subsequently published the results of this collaboration jointly with them, one article in a peer reviewed journal article and one paper in the proceedings of a conference. Dr. Nonaka is now a research scientist in the Center for Computational Sciences and Engineering (CCSE), which is also part of the National Energy Research Supercomputer Center (NERSC) at LBNL. This collaboration with researchers at LBNL also included having one of Professor Puckett's graduate students in the Graduate Group in Applied Mathematics (GGAM) at UCD, Sarah Williams, spend the summer working with Dr. Ann Almgren, who is a staff scientist in CCSE. As a result of this visit Sarah decided work on a problem suggested by the head of CCSE, Dr. John Bell, for her PhD thesis. Having finished all of the coursework and examinations required for a PhD, Sarah stayed at LBNL to work on her thesis under the guidance of Dr. Bell. Sarah finished her PhD thesis in June of 2007. Writing a PhD thesis while working at one of the University of California (UC) managed DOE laboratories is long established tradition at UC and Professor Puckett has always encouraged his students to consider doing this. Another one of Professor Puckett's graduate students in the GGAM at UCD, Christopher Algieri, was partially supported with funds from DE-FG02-03ER25579 while he wrote his MS thesis in which he analyzed and extended work originally published by Dr. Phillip Colella, the head of ANAG, and some of his colleagues. Chris Algieri is now employed as a staff member in Dr. Bill Collins' Climate Science Department in the Earth Sciences Division at LBNL working with computational models of climate change. Finally, it should be noted that the work conducted by Professor Puckett and his students Sarah Williams and Chris Algieri and described in this final report for DOE grant # DE-FC02-03ER25579 is closely related to work performed by Professor Puckett and his students under the auspices of Professor Puckett's DOE SciDAC grant DE-FC02-01ER25473 An Algorithmic and Software Framework for Applied Partial Differential Equations: A DOE SciDAC Integrated Software Infrastructure Center (ISIC). Dr. Colella was the lead PI for this SciDAC grant, which was comprised of several research groups from DOE national laboratories and five university PI's from five different universities. In theory Professor Puckett tried to use funds from the SciDAC grant to support work directly involved in implementing algorithms developed by members of his research group at UCD as software that might be of use to Puckett's SciDAC CoPIs. (For example, see the work reported in Section 2.2.2 of this final report.) However, since there is considerable lead time spent developing such algorithms before they are ready to become `software' and research plans and goals change as the research progresses, Professor Puckett supported each member of his research group partially with funds from the SciDAC APDEC ISIC DE-FC02-01ER25473 and partially with funds from this DOE MICS grant DE-FC02-03ER25579. This has necessarily resulted in a significant overlap of project areas that were funded by both grants. In particular, both Sarah Williams and Chris Algieri were supported partially with funds from grant # DE-FG02-03ER25579, for which this is the final report, and in part with funds from Professor Puckett's DOE SciDAC grant # DE-FC02-01ER25473. For example, Sarah Williams received support from DE-FC02- 01ER25473 and DE-FC02-03ER25579, both while at UCD taking cla

  20. Final Technical Report "Catalytic Hydrogenation of Carbon Monoxide and Olefin Oxidation" Grant number : DE-FG02-86ER13615

    SciTech Connect (OSTI)

    Wayland, B.B.

    2009-08-31

    Title: Catalytic Hydrogenation of Carbon Monoxide and Olefin Oxidation Grant No. DE-FG02-86ER13615 PI: Wayland, B. B. (wayland@sas.upenn.edu) Abstract Development of new mechanistic strategies and catalyst materials for activation of CO, H2, CH4, C2H4, O2, and related substrates relevant to the conversion of carbon monoxide, alkanes, and alkenes to organic oxygenates are central objectives encompassed by this program. Design and synthesis of metal complexes that manifest reactivity patterns associated with potential pathways for the hydrogenation of carbon monoxide through metallo-formyl (M-CHO), dimetal ketone (M-C(O)-M), and dimetal dionyl (M-C(O)-C(O)-M) species is one major focus. Hydrocarbon oxidation using molecular oxygen is a central goal for methane activation and functionalization as well as regioselective oxidation of olefins. Discovery of new reactivity patterns and control of selectivity are pursued through designing new metal complexes and adjusting reaction conditions. Variation of reaction media promotes distinct reaction pathways that control both reaction rates and selectivities. Dimetalloradical diporphyrin complexes preorganize transition states for substrate reactions that involve two metal centers and manifest large rate increases over mono-metalloradical reactions of hydrogen, methane, and other small molecule substrates. Another broad goal and recurring theme of this program is to contribute to the thermodynamic database for a wide scope of organo-metal transformations in a range of reaction media. One of the most complete descriptions of equilibrium thermodynamics for organometallic reactions in water and methanol is emerging from the study of rhodium porphyrin substrate reactions in aqueous and alcoholic media. Water soluble group nine metalloporphyrins manifest remarkably versatile substrate reactivity in aqueous and alcoholic media which includes producing rhodium formyl (Rh-CHO) and hydroxy methyl (Rh-CH2OH) species. Exploratory directions for this program include expending new strategies for anti-Markovnikov addition of water, alcohols, and amines with olefins, developing catalytic reactions of CO to give formamides and formic esters, and evaluating the potential for coupling reactions of CO to produce organic building blocks.

  1. Syntheses, structure and rare earth metal photoluminescence of new and known isostructural A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Ce, Pr, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu) compounds

    SciTech Connect (OSTI)

    Mohitkar, Shrikant A.; Kalpana, G.; Vidyasagar, K.

    2011-04-15

    Nine new A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Ce, Pr, Eu, Tb, Ho, Er, Tm, Yb, Lu) compounds have been synthesized by solid-state reactions. They are isostructural with six reported analogues of yttrium and other lanthanides and the monoclinic unit cell parameters of all fifteen of them vary linearly with the size of A{sup 3+} ion. Single crystal X-ray structures of eight A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Ce, Pr, Eu, Gd, Tb, Ho, Er, Tm) compounds have been determined. Neat A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Pr, Sm, Eu, Tb, Dy, Ho, Er, Tm) compounds exhibit characteristic rare earth metal photoluminescence. -- Graphical abstract: Among the fifteen isostructural A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=rare earth metal) molybdoantimonites, eight (A=Pr, Sm, Eu, Tb, Dy, Ho, Er, Tm) compounds exhibit neat characteristic lanthanide photoluminescence in the 200-800 nm range at room temperature. Display Omitted Research highlights: {yields} Syntheses of nine new A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Ce, Pr, Eu, Tb, Ho, Er, Tm, Yb, Lu) compounds. {yields} X-ray structures of eight A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Ce, Pr, Eu, Gd, Tb, Ho, Er, Tm) compounds. {yields} Photoluminescence of neat A{sub 2}Mo{sub 4}Sb{sub 2}O{sub 18} (A=Pr, Sm, Eu, Tb, Dy, Ho, Er, Tm) compounds.

  2. PyRAT (python radiography analysis tool): overview

    SciTech Connect (OSTI)

    Armstrong, Jerawan C; Temple, Brian A; Buescher, Kevin L

    2011-01-14

    PyRAT was developed as a quantitative tool for robustly characterizing objects from radiographs to solve problems such as the hybrid nonlinear inverse problem. The optimization software library that was used is the nonsmooth optimization by MADS algorithm (NOMAD). Some of PyRAT's features are: (1) hybrid nonlinear inverse problem with calculated x-ray spectrum and detector response; (2) optimization based inversion approach with goal of identifying unknown object configurations - MVO problem; (3) using functionalities of Python libraries for radiographic image processing and analysis; (4) using the Tikhonov regularization method of linear inverse problem to recover partial information of object configurations; (5) using a priori knowledge of problem solutions to define feasible region and discrete neighbor for the MVO problem - initial data analysis + material library {yields} a priori knowledge; and (6) using the NOMAD (C++ version) software in the object.

  3. Sex difference in the principal cytochrome P-450 for tributyltin metabolism in rats

    SciTech Connect (OSTI)

    Ohhira, Shuji [Department of Hygiene, Dokkyo University School of Medicine, Mibu-machi, Tochigi 321-0293 (Japan)]. E-mail: s-ohhira@dokkyomed.ac.jp; Enomoto, Mitsunori [Department of Hygiene, Dokkyo University School of Medicine, Mibu-machi, Tochigi 321-0293 (Japan); Matsui, Hisao [Department of Hygiene, Dokkyo University School of Medicine, Mibu-machi, Tochigi 321-0293 (Japan)

    2006-01-15

    Tributyltin is metabolized by cytochrome P-450 (CYP) system enzymes, and its metabolic fate may contribute to the toxicity of the chemical. In the present study, it is examined whether sex differences in the metabolism of tributyltin exist in rats. In addition, the in vivo and in vitro metabolism of tributyltin was investigated using rat hepatic CYP systems to confirm the principal CYP involved. A significant sex difference in metabolism occurred both in vivo and in vitro, suggesting that one of the CYPs responsible for tributyltin metabolism in rats is male specific or predominant at least. Eight cDNA-expressed rat CYPs, including typical phenobarbital (PB)-inducible forms and members of the CYP2C subfamily, were tested to determine their capability for tributyltin metabolism. Among the enzymes studied, a statistically significant dealkylation of tributyltin was mediated by CYP2C6 and 2C11. Furthermore, the sex difference in metabolism disappeared in vitro after anti-rat CYP2C11 antibody pretreatment because CYP2C11 is a major male-specific form in rats. These results indicate that CYP2C6 is the principal CYP for tributyltin metabolism in female rats, whereas CYP2C11 as well as 2C6 is involved in tributyltin metabolism in male rats, and it is suggested that CYP2C11 is responsible for the significant sex difference in the metabolism of tributyltin observed in rats.

  4. PyRAT - python radiography analysis tool (u)

    SciTech Connect (OSTI)

    Temple, Brian A; Buescher, Kevin L; Armstrong, Jerawan C

    2011-01-14

    PyRAT is a radiography analysis tool used to reconstruction images of unknown 1-0 objects. The tool is written in Python and developed for use on LINUX and Windows platforms. The tool is capable of performing nonlinear inversions of the images with minimal manual interaction in the optimization process. The tool utilizes the NOMAD mixed variable optimization tool to perform the optimization.

  5. Experimental and Theoretical Studies of Liquid-Solid and Liquid-Vapor Interfaces of Metals and Alloys, Grant DE-FG02-06ER46321

    SciTech Connect (OSTI)

    Rice, Stuart

    2012-09-27

    The research supported by ER46321 was designed to understand in microscopic detail the structures of the interfaces between liquid metals and alloys and solid media. The system chosen for study, because of detailed knowledge of the structure of the corresponding liquid alloy-vapor interface, was the interface between a Si crystal and a dilute alloy of Pb in Ga. Experimental study of the Si:PbGa interface was anticipated to be very difficult; it requires preparation of an interface between a liquid metal and a solid surface that is flat to better than a nanometer on the scale length of the x-ray coherence, alignment of the x-ray beam and the surface in the sub-micro radian regime, and the use of high energy x-rays to penetrate the window and reach the interface without disastrous loss of intensity. The experimental design was subject to compromises forced by the limit to the highest x-ray energy available at the ChemMatCARS beam-line, namely 30 keV, which reduced the scattered signal relative to what can be obtained with higher x-ray energy. Although considerable progress was achieved during the support period and its no-cost extension, the difficulties encountered prevented completion of the studies and the data collected are incomplete. These data hint at the existence of unexpected structural features of the interface, in particular that Pb dimers play an important role in the interfacial structure. These data provide a different picture of the interface from the pentagonal structure inferred to be present in the interface between pure Pb and Si 001 (Nature 408, 839 (2000)), but much like the Ga dimers in the interface between liquid Ga and the 100 face of diamond (Nature 390, 379 (1997), J. Chem. Phys. 123, 104703 (2005)). However, during the latter part of the support period significant progress was made in the theoretical description of the liquid metal-crystal interface. In particular, stimulated by the results of an experimental study of the interface between liquid Hg and the reconstructed (0001) face of sapphire, we developed an extension of the self-consistent quantum Monte Carlo scheme previously used to study the structure of the liquid metal-vapor interface. The calculated density distribution is in very good agreement with that inferred from the experimental data. We conclude, contrary to the original interpretation offered by Tamam et al (J. Phys. Chem. Lett. 2010, I, 1041-1045), thast to account for the difference in structure between the liquid Hg-vapor and liquid Hg-reconstructed (0001) Al{sub 2}O{sub 3} interfaces it is not necessary to assume there is charge transfer from the Hg to the Al{sub 2}O{sub 3}. Rather, the available experimental data are adequately reproduced when the van der Waals interaction of the Al and O atoms with Hg atoms and the exclusion of the electron density from the Al{sub 2}O{sub 3} via repulsion of the electrons from the closed shells of the ions in the solid are accounted for. We believe this interpretation will be applicable to a wide range of liquid metal-crystal interfaces.

  6. Luminescence properties of light-emitting diodes based on GaAs with the up-conversion Y{sub 2}O{sub 2}S:Er,Yb luminophor

    SciTech Connect (OSTI)

    Gruzintsev, A. N. [Russian Academy of Sciences, Institute of Problems of Microelectronics Technology (Russian Federation)], E-mail: gran@ipmt-hpm.ac.ru; Barthou, C.; Benalloul, P. [Institute des NanoSciences (France)

    2008-03-15

    Y{sub 2}O{sub 2}S luminophors doped with Er{sup 3+} and Yb{sup 3+} ions are produced by means of solid-phase synthesis and deposited onto standard AL123A infrared light-emitting diodes. When excited with 940 nm radiation from a light-emitting diode, the structures exhibit intense visible up-conversion luminescence. A maximal brightness of 2340 cd/m{sup 2} of green and red up-conversion luminescence at corresponding wavelengths around 550 and 600 nm is observed for the Y{sub 2}O{sub 2}S compound doped with 2 at % Er{sup 3+} ions and 6 at % Yb{sup 3+} ions. The ratio of the intensity of green (or red) up-conversion luminescence to the intensity of infrared Stokes luminescence increases with increasing applied voltage. The efficiency of visible emission of the light-emitting diode structures is {eta} = 1.2 lm/W at an applied voltage of 1.5 V.

  7. Proteomic analysis of rat cerebral cortex following subchronic acrolein toxicity

    SciTech Connect (OSTI)

    Rashedinia, Marzieh; Lari, Parisa; Abnous, Khalil; Hosseinzadeh, Hossein

    2013-10-01

    Acrolein, a member of reactive ?,?-unsaturated aldehydes, is a major environmental pollutant. Acrolein is also produced endogenously as a toxic by-product of lipid peroxidation. Because of high reactivity, acrolein may mediate oxidative damages to cells and tissues. It has been shown to be involved in a wide variety of pathological states including pulmonary, atherosclerosis and neurodegenerative diseases. In this study we employed proteomics approach to investigate the effects of subchronic oral exposures to 3 mg/kg of acrolein on protein expression profile in the brain of rats. Moreover effects of acrolein on malondialdehyde (MDA) levels and reduced glutathione (GSH) content were investigated. Our results revealed that treatment with acrolein changed levels of several proteins in diverse physiological process including energy metabolism, cell communication and transport, response to stimulus and metabolic process. Interestingly, several differentially over-expressed proteins, including ?-synuclein, enolase and calcineurin, are known to be associated with human neurodegenerative diseases. Changes in the levels of some proteins were confirmed by Western blot. Moreover, acrolein increases the level of MDA, as a lipid peroxidation biomarker and decreased GSH concentrations, as a non-enzyme antioxidant in the brain of acrolein treated rats. These findings suggested that acrolein induces the oxidative stress and lipid peroxidation in the brain, and so that may contribute to the pathophysiology of neurological disorders. - Highlights: Acrolein intoxication increased lipid peroxidation and deplete GSH in rat brain. Effect of acrolein on protein levels of cerebral cortex was analyzed by 2DE-PAGE. Levels of a number of proteins with different biological functions were increased.

  8. Effect of co-exposure and cadmium in rats

    SciTech Connect (OSTI)

    Tandon, S.K.; Tewari, P.C.

    1987-10-01

    Metabolism and toxicity of heavy metals may be influenced by certain factors such as protein malnutrition, essential element deficiency or alcoholism. Ethanol has been found to enhance the absorption of lead in body and alcoholics have been reported to be more susceptible to lead intoxication. As alcoholism may be common among industry workers and a significant section of population, who may be exposed to cadmium, it was considered of interest to investigate the influence of ethanol-cadmium co-exposure on cadmium sensitive hepatic, renal and serum enzymes, tissue accumulation of cadmium, essential trace element status and cadmium induced hepatic metallothione in synthesis in rats.

  9. ZERH Appraisal Process.pptx

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Home Certification As the cer tified Rat er for t his house, I cer t ify this house ... y Renewable Energy Ready Solar Hot Wat er Requir ement s This home was qualified via ...

  10. SU-E-T-492: Implementing a Method for Brain Irradiation in Rats Utilizing a Commercially Available Radiosurgery Irradiator

    SciTech Connect (OSTI)

    Cates, J; Drzymala, R

    2014-06-01

    Purpose: The purpose of the study was to implement a method for accurate rat brain irradiation using the Gamma Knife Perfexion unit. The system needed to be repeatable, efficient, and dosimetrically and spatially accurate. Methods: A platform (“rat holder”) was made such that it is attachable to the Leskell Gamma Knife G Frame. The rat holder utilizes two ear bars contacting bony anatomy and a front tooth bar to secure the rat. The rat holder fits inside of the Leskell localizer box, which utilizes fiducial markers to register with the GammaPlan planning system. This method allows for accurate, repeatable setup.A cylindrical phantom was made so that film can be placed axially in the phantom. We then acquired CT image sets of the rat holder and localizer box with both a rat and the phantom. Three treatment plans were created: a plan on the rat CT dataset, a phantom plan with the same prescription dose as the rat plan, and a phantom plan with the same delivery time as the rat plan. Results: Film analysis from the phantom showed that our setup is spatially accurate and repeatable. It is also dosimetrically accurate, with an difference between predicted and measured dose of 2.9%. Film analysis with prescription dose equal between rat and phantom plans showed a difference of 3.8%, showing that our phantom is a good representation of the rat for dosimetry purposes, allowing for +/- 3mm diameter variation. Film analysis with treatment time equal showed an error of 2.6%, which means we can deliver a prescription dose within 3% accuracy. Conclusion: Our method for irradiation of rat brain has been shown to be repeatable, efficient, and accurate, both dosimetrically and spatially. We can treat a large number of rats efficiently while delivering prescription doses within 3% at millimeter level accuracy.

  11. LANL12-RS-107J PYTHON Radiography Analysis Tool (PyRAT). Mid-Year

    Office of Scientific and Technical Information (OSTI)

    Deliverable Report for FY15 (Technical Report) | SciTech Connect LANL12-RS-107J PYTHON Radiography Analysis Tool (PyRAT). Mid-Year Deliverable Report for FY15 Citation Details In-Document Search Title: LANL12-RS-107J PYTHON Radiography Analysis Tool (PyRAT). Mid-Year Deliverable Report for FY15 This document is a mid-year report on a deliverable for the PYTHON Radiography Analysis Tool (PyRAT) for project LANL12-RS-107J in FY15. The deliverable is deliverable number 2 in the work package and

  12. Final report for Texas A&M University Group Contribution to DE-FG02-09ER25949/DE-SC0002505: Topology for Statistical Modeling of Petascale Data (and ASCR-funded collaboration between Sandia National Labs, Texas A&M University and University of Utah)

    SciTech Connect (OSTI)

    Rojas, Joseph Maurice

    2013-02-27

    We summarize the contributions of the Texas A\\&M University Group to the project (DE-FG02-09ER25949/DE-SC0002505: Topology for Statistical Modeling of Petascale Data - an ASCR-funded collaboration between Sandia National Labs, Texas A\\&M U, and U Utah) during 6/9/2011 -- 2/27/2013.

  13. Secondary structure of rat and human amylin across force fields

    SciTech Connect (OSTI)

    Hoffmann, Kyle Quynn; McGovern, Michael; Chiu, Chi -cheng; de Pablo, Juan J.; Paci, Emanuele

    2015-07-29

    The aggregation of human amylin has been strongly implicated in the progression of Type II diabetes. This 37-residue peptide forms a variety of secondary structures, including random coils, α-helices, and β-hairpins. The balance between these structures depends on the chemical environment, making amylin an ideal candidate to examine inherent biases in force fields. Rat amylin differs from human amylin by only 6 residues; however, it does not form fibrils. Therefore it provides a useful complement to human amylin in studies of the key events along the aggregation pathway. In this work, the free energy of rat and human amylin was determined as a function of α-helix and β-hairpin content for the Gromos96 53a6, OPLS-AA/L, CHARMM22/CMAP, CHARMM22*, Amberff99sb*-ILDN, and Amberff03w force fields using advanced sampling techniques, specifically bias exchange metadynamics. This work represents a first systematic attempt to evaluate the conformations and the corresponding free energy of a large, clinically relevant disordered peptide in solution across force fields. The NMR chemical shifts of rIAPP were calculated for each of the force fields using their respective free energy maps, allowing us to quantitatively assess their predictions. We show that the predicted distribution of secondary structures is sensitive to the choice of force-field: Gromos53a6 is biased towards β-hairpins, while CHARMM22/CMAP predicts structures that are overly α-helical. OPLS-AA/L favors disordered structures. Amberff99sb*-ILDN, AmberFF03w and CHARMM22* provide the balance between secondary structures that is most consistent with available experimental data. In contrast to previous reports, our findings suggest that the equilibrium conformations of human and rat amylin are remarkably similar, but that subtle differences arise in transient alpha-helical and beta-strand containing structures that the human peptide can more readily adopt. We hypothesize that these transient states enable dynamic pathways that facilitate the formation of aggregates and, eventually, amyloid fibrils.

  14. Secondary structure of rat and human amylin across force fields

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hoffmann, Kyle Quynn; McGovern, Michael; Chiu, Chi -cheng; de Pablo, Juan J.; Paci, Emanuele

    2015-07-29

    The aggregation of human amylin has been strongly implicated in the progression of Type II diabetes. This 37-residue peptide forms a variety of secondary structures, including random coils, α-helices, and β-hairpins. The balance between these structures depends on the chemical environment, making amylin an ideal candidate to examine inherent biases in force fields. Rat amylin differs from human amylin by only 6 residues; however, it does not form fibrils. Therefore it provides a useful complement to human amylin in studies of the key events along the aggregation pathway. In this work, the free energy of rat and human amylin wasmore » determined as a function of α-helix and β-hairpin content for the Gromos96 53a6, OPLS-AA/L, CHARMM22/CMAP, CHARMM22*, Amberff99sb*-ILDN, and Amberff03w force fields using advanced sampling techniques, specifically bias exchange metadynamics. This work represents a first systematic attempt to evaluate the conformations and the corresponding free energy of a large, clinically relevant disordered peptide in solution across force fields. The NMR chemical shifts of rIAPP were calculated for each of the force fields using their respective free energy maps, allowing us to quantitatively assess their predictions. We show that the predicted distribution of secondary structures is sensitive to the choice of force-field: Gromos53a6 is biased towards β-hairpins, while CHARMM22/CMAP predicts structures that are overly α-helical. OPLS-AA/L favors disordered structures. Amberff99sb*-ILDN, AmberFF03w and CHARMM22* provide the balance between secondary structures that is most consistent with available experimental data. In contrast to previous reports, our findings suggest that the equilibrium conformations of human and rat amylin are remarkably similar, but that subtle differences arise in transient alpha-helical and beta-strand containing structures that the human peptide can more readily adopt. We hypothesize that these transient states enable dynamic pathways that facilitate the formation of aggregates and, eventually, amyloid fibrils.« less

  15. LANL12-RS-107J PYTHON Radiography Analysis Tool (PyRAT). Mid...

    Office of Scientific and Technical Information (OSTI)

    LANL12-RS-107J PYTHON Radiography Analysis Tool (PyRAT). Mid-Year Deliverable Report for FY15 Citation Details In-Document Search Title: LANL12-RS-107J PYTHON Radiography Analysis ...

  16. LANL12-RS-107J PYTHON Radiography Analysis Tool (PyRAT). Mid...

    Office of Scientific and Technical Information (OSTI)

    project LANL12-RS-107J in FY15. The deliverable is deliverable number 2 in the work package and is titled "Add the ability to read in more types of image file formats in PyRAT"....

  17. Antimuscarinic effects of chloroquine in rat pancreatic acini

    SciTech Connect (OSTI)

    Habara, Y.; Williams, J.A.; Hootman, S.R.

    1986-06-13

    Chloroquine inhibited carbachol-induced amylase release in a dose-dependent fashion in rat pancreatic acini; cholecystokinin- and bombesin-induced secretory responses were almost unchanged by the antimalarial drug. The inhibition of carbachol-induced amylase release by chloroquine was competitive in nature with a K/sub i/ of 11.7 ..mu..M. Chloroquine also inhibited (/sup 3/H)N-methylscopolamine binding to acinar muscarinic receptors. The IC/sub 50/ for chloroquine inhibition of (/sup 3/H)N-methylscopolamine binding was lower than that for carbachol or the other antimalarial drugs, quinine and quinidine. These results demonstrate that chloroquine is a muscarinic receptor antagonist in the exocrine pancreas.

  18. Adverse testicular effects of Botox in mature rats

    SciTech Connect (OSTI)

    Breikaa, Randa M.; Mosli, Hisham A.; Nagy, Ayman A.; Abdel-Naim, Ashraf B.

    2014-03-01

    Botox injections are taking a consistently increasing place in urology. Intracremasteric injections, particularly, have been applied for cryptorchidism and painful testicular spasms. Studies outlining their safety for this use are, however, scanty. Thus, the present study aimed at evaluating possible testicular toxicity of Botox injections and their effect on male fertility. Mature rats were given intracremasteric Botox injections (10, 20 and 40 U/kg) three times in a two-week interval. Changes in body and testes weights were examined and gonadosomatic index compared to control group. Semen quality, sperm parameters, fructose, protein, cholesterol and triglycerides contents were assessed. Effects on normal testicular function were investigated by measuring testosterone levels and changes in enzyme activities (lactate dehydrogenase-X and acid phosphatase). To draw a complete picture, changes in oxidative and inflammatory states were examined, in addition to the extent of connective tissue deposition between seminiferous tubules. In an attempt to have more accurate information about possible spermatotoxic effects of Botox, flowcytometric analysis and histopathological examination were carried out. Botox-injected rats showed altered testicular physiology and function. Seminiferous tubules were separated by dense fibers, especially with the highest dose. Flowcytometric analysis showed a decrease in mature sperms and histopathology confirmed the findings. The oxidative state was, however, comparable to control group. This study is the first to show that intracremasteric injections of Botox induce adverse testicular effects evidenced by inhibited spermatogenesis and initiation of histopathological changes. In conclusion, decreased fertility may be a serious problem Botox injections could cause. - Highlights: Botox injections are the trend nowadays, for both medical and non-medical uses. They were recently suggested for cryptorchidism and testicular spasms. This study outlines possible testicular adverse effects of these injections. Botox affected normal testicular function and physiology. Infertility is a serious problem that Botox injections could cause.

  19. Palmitate attenuates osteoblast differentiation of fetal rat calvarial cells

    SciTech Connect (OSTI)

    Yeh, Lee-Chuan C.; Ford, Jeffery J.; Lee, John C.; Adamo, Martin L.

    2014-07-18

    Highlights: • Palmitate inhibits osteoblast differentiation. • Fatty acid synthase. • PPARγ. • Acetyl Co-A carboxylase inhibitor TOFA. • Fetal rat calvarial cell culture. - Abstract: Aging is associated with the accumulation of ectopic lipid resulting in the inhibition of normal organ function, a phenomenon known as lipotoxicity. Within the bone marrow microenvironment, elevation in fatty acid levels may produce an increase in osteoclast activity and a decrease in osteoblast number and function, thus contributing to age-related osteoporosis. However, little is known about lipotoxic mechanisms in intramembraneous bone. Previously we reported that the long chain saturated fatty acid palmitate inhibited the expression of the osteogenic markers RUNX2 and osteocalcin in fetal rat calvarial cell (FRC) cultures. Moreover, the acetyl CoA carboxylase inhibitor TOFA blocked the inhibitory effect of palmitate on expression of these two markers. In the current study we have extended these observations to show that palmitate inhibits spontaneous mineralized bone formation in FRC cultures in association with reduced mRNA expression of RUNX2, alkaline phosphatase, osteocalcin, and bone sialoprotein and reduced alkaline phosphatase activity. The effects of palmitate on osteogenic marker expression were inhibited by TOFA. Palmitate also inhibited the mRNA expression of fatty acid synthase and PPARγ in FRC cultures, and as with osteogenic markers, this effect was inhibited by TOFA. Palmitate had no effect on FRC cell proliferation or apoptosis, but inhibited BMP-7-induced alkaline phosphatase activity. We conclude that palmitate accumulation may lead to lipotoxic effects on osteoblast differentiation and mineralization and that increases in fatty acid oxidation may help to prevent these lipotoxic effects.

  20. Linkage maps of rat chromosomes 15, 16, 17, 19, and X

    SciTech Connect (OSTI)

    Du, Ying; Remmers, E.F.; Goldmuntz, E.A.

    1996-02-15

    This article reports on novel linkage maps of rat chromosomes 15, 16, 17, 19, and X. Such a map is needed to further the usefulness of the rat as a biological model in hereditary diseases. The linkage map results produced polymorphic markers which were associated with eight known genes and fourteen anonymous loci. This was accomplished using PCR analysis of somatic cell hybrids or previously listed loci locations. 15 refs., 1 fig., 1 tab.

  1. Ten polymorphic DNA loci, including five in the rat MHC (RT1) region, form a single linkage group on rat chromosome 20

    SciTech Connect (OSTI)

    Remmers, E.F.; Du, Y.; Zha, H.; Goldmuntz, E.A.; Wilder, R.L.

    1995-03-01

    We have described ten markers for polymorphic loci on rat chromosome 20, including five in the rat MHC (RT1) region. These markers formed a single linkage group spanning a recombination distance of 0.40. The markers identified five expressed gene loci - RT1.N1 (thymus leukemia antigen 1), Tnfa (tumor necrosis factor {alpha}), Hspa1 (heat shock protein 70), Ggt1 ({gamma} glutamyl-transferase 1), and Prkacn2 (protein kinase C catalytic subunit binding inhibitor 2), two loci with sequences that are related to expressed genes - RT1.Aw2 (sequence related to a non-RT1A class I {alpha} chain) and Mt21 (sequence related to metallothionein 2), and three anonymous loci - D20Arb548, D20Arb234, and D20Arb249. These polymorphic markers should facilitate mapping studies and genetic monitoring of inbred rat strains. 18 refs., 2 figs., 3 tabs.

  2. Automated whole-genome multiple alignment of rat, mouse, and human

    SciTech Connect (OSTI)

    Brudno, Michael; Poliakov, Alexander; Salamov, Asaf; Cooper, Gregory M.; Sidow, Arend; Rubin, Edward M.; Solovyev, Victor; Batzoglou, Serafim; Dubchak, Inna

    2004-07-04

    We have built a whole genome multiple alignment of the three currently available mammalian genomes using a fully automated pipeline which combines the local/global approach of the Berkeley Genome Pipeline and the LAGAN program. The strategy is based on progressive alignment, and consists of two main steps: (1) alignment of the mouse and rat genomes; and (2) alignment of human to either the mouse-rat alignments from step 1, or the remaining unaligned mouse and rat sequences. The resulting alignments demonstrate high sensitivity, with 87% of all human gene-coding areas aligned in both mouse and rat. The specificity is also high: <7% of the rat contigs are aligned to multiple places in human and 97% of all alignments with human sequence > 100kb agree with a three-way synteny map built independently using predicted exons in the three genomes. At the nucleotide level <1% of the rat nucleotides are mapped to multiple places in the human sequence in the alignment; and 96.5% of human nucleotides within all alignments agree with the synteny map. The alignments are publicly available online, with visualization through the novel Multi-VISTA browser that we also present.

  3. Anti-inflammatory activity of methyl palmitate and ethyl palmitate in different experimental rat models

    SciTech Connect (OSTI)

    Saeed, Noha M.; El-Demerdash, Ebtehal; Abdel-Rahman, Hanaa M.; Algandaby, Mardi M.; Al-Abbasi, Fahad A.; Abdel-Naim, Ashraf B.

    2012-10-01

    Methyl palmitate (MP) and ethyl palmitate (EP) are naturally occurring fatty acid esters reported as inflammatory cell inhibitors. In the current study, the potential anti-inflammatory activity of MP and EP was evaluated in different experimental rat models. Results showed that MP and EP caused reduction of carrageenan-induced rat paw edema in addition to diminishing prostaglandin E2 (PGE2) level in the inflammatory exudates. In lipopolysaccharide (LPS)-induced endotoxemia in rats, MP and EP reduced plasma levels of tumor necrosis factor-? (TNF-?) and interleukin-6 (IL-6). MP and EP decreased NF-?B expression in liver and lung tissues and ameliorated histopathological changes caused by LPS. Topical application of MP and EP reduced ear edema induced by croton oil in rats. In the same animal model, MP and EP reduced neutrophil infiltration, as indicated by decreased myeloperoxidase (MPO) activity. In conclusion, this study demonstrates the effectiveness of MP and EP in combating inflammation in several experimental models. -- Highlights: ? Efficacy of MP and EP in combating inflammation was displayed in several models. ? MP and EP reduced carrageenan-induced rat paw edema and prostaglandin E2 level. ? MP and EP decreased TNF-? and IL-6 levels in experimental endotoxemia. ? MP and EP reduced NF-?B expression and histological changes in rat liver and lung. ? MP and EP reduced croton oil-induced ear edema and neutrophil infiltration.

  4. Zinc content of selected tissues and taste perception in rats fed zinc deficient and zinc adequate rations

    SciTech Connect (OSTI)

    Boeckner, L.S.; Kies, C.

    1986-03-05

    The objective of the study was to determine the effects of feeding zinc sufficient and zinc deficient rations on taste sensitivity and zinc contents of selected organs in rats. The 36 Sprague-Dawley male weanling rats were divided into 2 groups and fed zinc deficient or zinc adequate rations. The animals were subjected to 4 trial periods in which a choice of deionized distilled water or a solution of quinine sulfate at 1.28 x 10/sup -6/ was given. A randomized schedule for rat sacrifice was used. No differences were found between zinc deficient and zinc adequate rats in taste preference aversion scores for quinine sulfate in the first three trial periods; however, in the last trial period rats in the zinc sufficient group drank somewhat less water containing quinine sulfate as a percentage of total water consumption than did rats fed the zinc deficient ration. Significantly higher zinc contents of kidney, brain and parotid salivary glands were seen in zinc adequate rats compared to zinc deficient rats at the end of the study. However, liver and tongue zinc levels were lower for both groups at the close of the study than were those of rats sacrificed at the beginning of the study.

  5. Final Report for U.S. DOE GRANT No. DEFG02-96ER41015 November 1, 2010 - April 30, 2013 entitled HIGH ENERGY ACCELERATOR AND COLLIDING BEAM USER GROUP at the UNIVERSITY of MARYLAND

    SciTech Connect (OSTI)

    Hadley, Nicholas; Jawahery, Abolhassan; Eno, Sarah C; Skuja, Andris; Baden, Andrew; Roberts, Douglas

    2013-07-26

    We have #12;finished the third year of a three year grant cycle with the U.S. Department of Energy for which we were given a #12;five month extension (U.S. D.O.E. Grant No. DEFG02-96ER41015). This document is the fi#12;nal report for this grant and covers the period from November 1, 2010 to April 30, 2013. The Maryland program is administered as a single task with Professor Nicholas Hadley as Principal Investigator. The Maryland experimental HEP group is focused on two major research areas. We are members of the CMS experiment at the LHC at CERN working on the physics of the Energy Frontier. We are also analyzing the data from the Babar experiment at SLAC while doing design work and R&D towards a Super B experiment as part of the Intensity Frontier. We have recently joined the LHCb experiment at CERN. We concluded our activities on the D#31; experiment at Fermilab in 2009.

  6. In vitro dermal absorption of pyrethroid pesticides in human and rat skin

    SciTech Connect (OSTI)

    Hughes, Michael F.; Edwards, Brenda C.

    2010-07-15

    Dermal exposure to pyrethroid pesticides can occur during manufacture and application. This study examined the in vitro dermal absorption of pyrethroids using rat and human skin. Dermatomed skin from adult male Long Evans rats or human cadavers was mounted in flow-through diffusion cells, and radiolabeled bifenthrin, deltamethrin or cis-permethrin was applied in acetone to the skin. Fractions of receptor fluid were collected every 4 h. At 24 h, the skins were washed with soap and water to remove unabsorbed chemical. The skin was then solubilized. Two additional experiments were performed after washing the skin; the first was tape-stripping the skin and the second was the collection of receptor fluid for an additional 24 h. Receptor fluid, skin washes, tape strips and skin were analyzed for radioactivity. For rat skin, the wash removed 53-71% of the dose and 26-43% remained in the skin. The cumulative percentage of the dose at 24 h in the receptor fluid ranged from 1 to 5%. For human skin, the wash removed 71-83% of the dose and 14-25% remained in the skin. The cumulative percentage of the dose at 24 h in the receptor fluid was 1-2%. Tape-stripping removed 50-56% and 79-95% of the dose in rat and human skin, respectively, after the wash. From 24-48 h, 1-3% and about 1% of the dose diffused into the receptor fluid of rat and human skin, respectively. The pyrethroids bifenthrin, deltamethrin and cis-permethrin penetrated rat and human skin following dermal application in vitro. However, a skin wash removed 50% or more of the dose from rat and human skin. Rat skin was more permeable to the pyrethroids than human skin. Of the dose in skin, 50% or more was removed by tape-stripping, suggesting that permeation of pyrethroids into viable tissue could be impeded. The percentage of the dose absorbed into the receptor fluid was considerably less than the dose in rat and human skin. Therefore, consideration of the skin type used and fractions analyzed are important when using in vitro dermal absorption data for risk assessment.

  7. Spatially resolved penetration depth measurements and vortex manipulation in the ferromagnetic superconductor ErNi2B2C

    SciTech Connect (OSTI)

    Wulferding, Dirk; Yang, Ilkyu; Yang, Jinho; Lee, Minkyung; Choi, Hee Cheul; Bud'ko, Sergey L.; Canfield, Paul C.; Yeom, Han Woong; Kim, Jeehoon

    2015-07-31

    We present a local probe study of the magnetic superconductor ErNi2B2C, using magnetic force microscopy at sub-Kelvin temperatures. ErNi2B2C is an ideal system to explore the effects of concomitant superconductivity and ferromagnetism. At 500 mK, far below the transition to a weakly ferromagnetic state, we directly observe a structured magnetic background on the micrometer scale. We determine spatially resolved absolute values of the magnetic penetration depth ? and study its temperature dependence as the system undergoes magnetic phase transitions from paramagnetic to antiferromagnetic, and to weak ferromagnetic, all within the superconducting regime. We estimate the absolute pinning force of Abrikosov vortices, which shows a position dependence and temperature dependence as well, and discuss the possibility of the purported spontaneous vortex formation.

  8. Cadmium effect on microsomal drug-metabolizing enzyme activity in rat livers with respect to differences in age and sex

    SciTech Connect (OSTI)

    Ando, M.

    1982-04-01

    The effect of cadmium on the hepatic microsomal drug-metabolizing enzyme system was investigated. Cadmium chloride caused the conversion of cytochrome P-450 to P-420 in rat liver microsomes. The destruction of cytochrome P-450 by cadmium caused the reduction of microsomal drug-metabolizing enzyme activity and prolonged the pentobarbital sleeping time. There is a sex-related difference in the ability of cadmium to inhibit the hepatic drug metabolism in rats: male rats are more sensitive to cadmium than females. The effective period when cadmium prolonged their sleep depended upon the age of rats; older rats were more sensitive to cadmium than younger ones. The maximum increase of sleeping time depended upon the dose level of cadium, and the rate constant of the equations seems to depend upon the age of the animals.

  9. Unraveling the mechanism of neuroprotection of curcumin in arsenic induced cholinergic dysfunctions in rats

    SciTech Connect (OSTI)

    Srivastava, Pranay; Yadav, Rajesh S.; Chandravanshi, Lalit P.; Shukla, Rajendra K.; Dhuriya, Yogesh K.; Chauhan, Lalit K.S.; Dwivedi, Hari N.; Pant, Aditiya B.; Khanna, Vinay K.

    2014-09-15

    Earlier, we found that arsenic induced cholinergic deficits in rat brain could be protected by curcumin. In continuation to this, the present study is focused to unravel the molecular mechanisms associated with the protective efficacy of curcumin in arsenic induced cholinergic deficits. Exposure to arsenic (20 mg/kg body weight, p.o) for 28 days in rats resulted to decrease the expression of CHRM2 receptor gene associated with mitochondrial dysfunctions as evident by decrease in the mitochondrial membrane potential, activity of mitochondrial complexes and enhanced apoptosis both in the frontal cortex and hippocampus in comparison to controls. The ultrastructural images of arsenic exposed rats, assessed by transmission electron microscope, exhibited loss of myelin sheath and distorted cristae in the mitochondria both in the frontal cortex and hippocampus as compared to controls. Simultaneous treatment with arsenic (20 mg/kg body weight, p.o) and curcumin (100 mg/kg body weight, p.o) for 28 days in rats was found to protect arsenic induced changes in the mitochondrial membrane potential and activity of mitochondrial complexes both in frontal cortex and hippocampus. Alterations in the expression of pro- and anti-apoptotic proteins and ultrastructural damage in the frontal cortex and hippocampus following arsenic exposure were also protected in rats simultaneously treated with arsenic and curcumin. The data of the present study reveal that curcumin could protect arsenic induced cholinergic deficits by modulating the expression of pro- and anti-apoptotic proteins in the brain. More interestingly, arsenic induced functional and ultrastructural changes in the brain mitochondria were also protected by curcumin. - Highlights: • Neuroprotective mechanism of curcumin in arsenic induced cholinergic deficits studied • Curcumin protected arsenic induced enhanced expression of stress markers in rat brain • Arsenic compromised mitochondrial electron transport chain protected by curcumin • Functional and structural changes in mitochondria by arsenic protected by curcumin.

  10. Age-dependent inhibition of pentobarbital sleeping time by ozone in mice and rats

    SciTech Connect (OSTI)

    Canada, A.T.; Calabrese, E.J.; Leonard, D.

    1986-09-01

    The effect of age on the metabolism of pentobarbital in mice and rats was investigated following exposure to 0.3 ppm of ozone for 3.75 hr. Young animals were 2.5 months of age and the mature were 18 months. The pentobarbital sleeping time was significantly prolonged following the ozone exposure in both the mice and rats when compared with an air control. No ozone effect on sleeping time was found in the young animals. The results indicate that there may be an age-related sensitivity to the occurrence of ozone-related inhibition of pentobarbital metabolism.

  11. doe-er-0494t.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  12. Final Technical Report, DOE/ER/64323

    SciTech Connect (OSTI)

    Valocchi, Albert J. University of Illinois, Dept of Civil & Environ Engr

    2013-06-05

    The DOE SciDAC program funded a team that developed PFLOTRAN, the next-generation (â??peta-scaleâ??) massively parallel, multiphase, multicomponent reactive flow and transport code. These codes are required to improve understanding and risk management of subsurface contaminant migration and geological sequestration of carbon dioxide. The important fate and transport processes occurring in the subsurface span a wide range of spatial and temporal scales, and involve nonlinear interactions among many different chemical constituents. Due to the complexity of this problem, modeling subsurface processes normally requires simplifying assumptions. However, tools of advanced scientific computing that have been used in other areas such as energy and materials research can also help address challenging problems in the environmental and geoscience fields. The overall project was led by Los Alamos National Laboratory and included Argonne, Oak Ridge and Pacific Northwest National Laboratories, in addition to the University of Illinois. This report summarizes the results of the research done at the University of Illinois, which focused on improvements to the underlying physical and computational modeling of certain transport and mixing processes.

  13. Final Report for DOE Award ER25756

    SciTech Connect (OSTI)

    Kesselman, Carl

    2014-11-17

    The SciDAC-funded Center for Enabling Distributed Petascale Science (CEDPS) was established to address technical challenges that arise due to the frequent geographic distribution of data producers (in particular, supercomputers and scientific instruments) and data consumers (people and computers) within the DOE laboratory system. Its goal is to produce technical innovations that meet DOE end-user needs for (a) rapid and dependable placement of large quantities of data within a distributed high-performance environment, and (b) the convenient construction of scalable science services that provide for the reliable and high-performance processing of computation and data analysis requests from many remote clients. The Center is also addressing (c) the important problem of troubleshooting these and other related ultra-high-performance distributed activities from the perspective of both performance and functionality

  14. NASA is operating two Lockheed ER-2

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Mis- sion Directorate. The aircraft, based at NASA's Dryden Flight Research Center, Edwards, Calif., collect information about our surroundings, including Earth resources,...

  15. FinalReport_01ER41190.dvi

    Office of Scientific and Technical Information (OSTI)

    in the discovery during the years 2001-2003 that quark-gluon plasma (QGP) created in nuclear collisions at RHIC behaves like an almost perfect liquid with minimal viscosity. ...

  16. Final Scientific Report for ER41087

    SciTech Connect (OSTI)

    Hiller, John R.

    2013-08-23

    The primary focus of the work was the development of methods for the nonperturbative solution of quantum chromodynamics (QCD) in a form that yields wave functions for the eigenstates, from which hadronic properties can be computed. The principal approach was to use a light-front Hamiltonian formulation. In light-front coordinates, t+z/c plays the role of time, with t the ordinary time, z a space direction, and c the speed of light. This leads to a relativistic formulation that retains useful characteristics of nonrelativistic treatments. A bound state of many constituents can be represented by wave functions that define probabilities for each possible arrangement of internal momenta. These functions satisfy integral equations that can be approximated numerically to yield a matrix representation. The matrix problem can be solved by iterative methods. The approximate wave functions can then be used to compute properties of the bound state. Methods have been developed for model theories and gauge theories, including quantum electrodynamics and theories that are supersymmetric. The work has required the development of new numerical algorithms and computer codes for singular integral equations and eigenvalue problems. A key aspect of the work is the construction of practical procedures for nonperturbative regularization and renormalization. Two methods of regularization have been studied. One is the addition of heavy Pauli--Villars (PV) particles to the Lagrangian, with their metrics and couplings tuned to provide the necessary cancellations in the regularization. The other method of regularization is the addition of supersymmetric partners, to extend a theory to a supersymmetric form. The supersymmetric theories were solved by the supersymmetric discrete light-cone quantization (SDLCQ) method. The most significant accomplishments of the project were the SDLCQ calculation of direct evidence for a Maldacena duality conjecture, construction of a practical light-front quantization for QED in an arbitrary covariant gauge, and invention of the light-front coupled-cluster method, designed to eliminate the need for Fock-space truncations.

  17. Inhalation developmental toxicology studies: Teratology study of tetrahydrofuran in mice and rats: Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Evanoff, J.J.; Stoney, K.H.; Westerberg, R.B.; Rommereim, R.L.; Weigel, R.J.

    1988-08-01

    Tetrahydrofuran (THF), a four-carbon cyclic ether, is widely used as an industrial solvent. Although it has been used in large quantities for many years, few long-term toxicology studies, and no reproductive or developmental studies, have been conducted on THF. This study addresses the potential for THF to cause developmental toxicity in rodents by exposing Sprague-Dawley rats and Swiss (CD-1) mice to 0, 600, 1800, or 5000 ppm tetrahydrofuran (THF) vapors, 6 h/day, 7 dy/wk. Each treatment group consisted of 10 virgin females (for comparison), and approx.33 positively mated rats or mice. Positively mated mice were exposed on days 6--17 of gestation (dg), and rats on 6--19 dg. The day of plug or sperm detection was designated as O dg. Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice (rats, 20 dg; mice, 18 dg). Implants were enumerated and their status recorded and live fetuses were examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. 27 refs., 6 figs., 23 tabs.

  18. Inhalation developmental toxicology studies: Teratology study of acetone in mice and rats: Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Evanoff, J.J.; Rommereim, R.L.; Stoney, K.H.; Weigel, R.J.; Westerberg, R.B.

    1988-11-01

    Acetone, an aliphatic ketone, is a ubiquitous industrial solvent and chemical intermediate; consequently, the opportunity for human exposure is high. The potential for acetone to cause developmental toxicity was assessed in Sprague-Dawley rats exposed to 0, 440, 2200, or 11000 ppm, and in Swiss (CD-1) mice exposed to 0, 440, 2200, and 6600 ppm acetone vapors, 6 h/day, 7 days/week. Each of the four treatment groups consisted of 10 virgin females (for comparison), and approx.32 positively mated rats or mice. Positively mated mice were exposed on days 6-17 of gestation (dg), and rats on 6-19 dg. The day of plug or sperm detection was designated as 0 dg. Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice (rats, 20 dg; mice, 18 dg). Implants were enumerated and their status recorded. Live fetuses were sexed and examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. 46 refs., 6 figs., 27 tabs.

  19. Inhalation developmental toxicology studies: Teratology study of isoprene in mice and rats: Final report

    SciTech Connect (OSTI)

    Mast, T.J.; Evanoff, J.J.; Stoney, K.H.; Westerberg, R.B.; Rommereim, R.L.; Weigel, R.J.

    1989-01-01

    Isoprene, a reactive, branched diene, is used in large quantities in the manufacture of polyisoprene and as a copolymer in the synthesis of butyl rubber. The potential for isoprene to cause developmental toxicity was assessed in rodents, by exposing four groups each of Sprague-Dawley rats and Swiss (CD-1) mice to 0, 280, 1400, or 7000 ppM isoprene vapors, 6 h/day, 7 day/wk. Each treatment group consisted of 10 virgin females (for comparison), and approx.30 positively mated rats or mice. Positively mated mice were exposed on days 6-17 of gestation (dg), and rats on 6-19 dg. The day of plug or sperm detection was designated as 0 dg. Body weights were obtained throughout the study period, and uterine and fetal body weights were obtained at sacrifice (rats, 20 dg; mice, 18 dg). Implants were enumerated and their status recorded. Live fetuses were sexed and examined for gross, visceral, skeletal, and soft-tissue craniofacial defects. 31 refs., 6 figs., 19 tabs.

  20. Gamma Knife irradiation method based on dosimetric controls to target small areas in rat brains

    SciTech Connect (OSTI)

    Constanzo, Julie; Paquette, Benoit; Charest, Gabriel; Masson-Côté, Laurence; Guillot, Mathieu

    2015-05-15

    Purpose: Targeted and whole-brain irradiation in humans can result in significant side effects causing decreased patient quality of life. To adequately investigate structural and functional alterations after stereotactic radiosurgery, preclinical studies are needed. The purpose of this work is to establish a robust standardized method of targeted irradiation on small regions of the rat brain. Methods: Euthanized male Fischer rats were imaged in a stereotactic bed, by computed tomography (CT), to estimate positioning variations relative to the bregma skull reference point. Using a rat brain atlas and the stereotactic bregma coordinates obtained from CT images, different regions of the brain were delimited and a treatment plan was generated. A single isocenter treatment plan delivering ≥100 Gy in 100% of the target volume was produced by Leksell GammaPlan using the 4 mm diameter collimator of sectors 4, 5, 7, and 8 of the Gamma Knife unit. Impact of positioning deviations of the rat brain on dose deposition was simulated by GammaPlan and validated with dosimetric measurements. Results: The authors’ results showed that 90% of the target volume received 100 ± 8 Gy and the maximum of deposited dose was 125 ± 0.7 Gy, which corresponds to an excellent relative standard deviation of 0.6%. This dose deposition calculated with GammaPlan was validated with dosimetric films resulting in a dose-profile agreement within 5%, both in X- and Z-axes. Conclusions: The authors’ results demonstrate the feasibility of standardizing the irradiation procedure of a small volume in the rat brain using a Gamma Knife.

  1. Sex- and dose-dependency in the pharmacokinetics and pharmacodynamics of (+)-methamphetamine and its metabolite (+)-amphetamine in rats

    SciTech Connect (OSTI)

    Milesi-Halle, Alessandra [Department of Pharmacology and Toxicology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham 611, Little Rock, AR 72205 (United States); Hendrickson, Howard P. [Department of Pharmacology and Toxicology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham 611, Little Rock, AR 72205 (United States); Laurenzana, Elizabeth M. [Department of Pharmacology and Toxicology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham 611, Little Rock, AR 72205 (United States); Gentry, W. Brooks [Department of Pharmacology and Toxicology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham 611, Little Rock, AR 72205 (United States); Department of Anesthesiology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Owens, S. Michael [Department of Pharmacology and Toxicology, College of Medicine, University of Arkansas for Medical Sciences, 4301 West Markham 611, Little Rock, AR 72205 (United States)]. E-mail: mowens@uams.edu

    2005-12-15

    These studies investigated how (+)-methamphetamine (METH) dose and rat sex affect the pharmacological response to METH in Sprague-Dawley rats. The first set of experiments determined the pharmacokinetics of METH and its pharmacologically active metabolite (+)-amphetamine (AMP) in male and female Sprague-Dawley rats after 1.0 and 3.0 mg/kg METH doses. The results showed significant sex-dependent changes in METH pharmacokinetics, and females formed significantly lower amounts of AMP. While the area under the serum concentration-time curve in males increased proportionately with the METH dose, the females showed a disproportional increase. The sex differences in systemic clearance, renal clearance, volume of distribution, and percentage of unchanged METH eliminated in the urine suggested dose-dependent pharmacokinetics in female rats. The second set of studies sought to determine the behavioral implications of these pharmacokinetic differences by quantifying locomotor activity in male and female rats after saline, 1.0, and 3.0 mg/kg METH. The results showed sex- and dose-dependent differences in METH-induced locomotion, including profound differences in the temporal profile of effects at higher dose. These findings show that the pharmacokinetic and metabolic profile of METH (slower METH clearance and lower AMP metabolite formation) plays a significant role in the differential pharmacological response to METH in male and female rats.

  2. Nicotinic acid increases the lipid content of rat brain synaptosomes. [Ethanol effects

    SciTech Connect (OSTI)

    Basilio, C.; Flores, M.

    1989-02-09

    Chronic administration of nicotinic acid (NA) increase hepatic lipids and potentiates a similar effect induced by ethanol. The amethystic properties of NA promoted us to study its effects on the lipid content of brain synaptosomes of native and ethanol treated rats. Groups of 10 Sprague-Dawley female rats received i.p. either saline, ethanol (4g/kg), NA (50mg/kg), or a mixture of both compounds once a week during 3 weeks. The sleeping time (ST) of the animals receiving ethanol was recorded, brain synaptosomes of all groups were prepared and total lipids (TL) and cholesterol (Chol) content were determined. NA, ethanol and ethanol + NA markedly increased both TL and Chol of synaptosomes. Animals treated with ethanol or ethanol + NA developed tolerance. The group treated with ethanol-NA showed the highest Chol content and slept significantly less than the one treated with ethanol alone indicating that the changes induced by NA favored the appearance of tolerance.

  3. Lipidomic changes in rat liver after long-term exposure to ethanol

    SciTech Connect (OSTI)

    Fernando, Harshica; Bhopale, Kamlesh K.; Kondraganti, Shakuntala; Kaphalia, Bhupendra S.; Shakeel Ansari, G.A.

    2011-09-01

    Alcoholic liver disease (ALD) is a serious health problem with significant morbidity and mortality. In this study we examined the progression of ALD along with lipidomic changes in rats fed ethanol for 2 and 3 months to understand the mechanism, and identify possible biomarkers. Male Fischer 344 rats were fed 5% ethanol or caloric equivalent of maltose-dextrin in a Lieber-DeCarli diet. Animals were killed at the end of 2 and 3 months and plasma and livers were collected. Portions of the liver were fixed for histological and immunohistological studies. Plasma and the liver lipids were extracted and analyzed by nuclear magnetic resonance (NMR) spectroscopy. A time dependent fatty infiltration was observed in the livers of ethanol-fed rats. Mild inflammation and oxidative stress were observed in some ethanol-fed rats at 3 months. The multivariate and principal component analysis of proton and phosphorus NMR spectroscopy data of extracted lipids from the plasma and livers showed segregation of ethanol-fed groups from the pair-fed controls. Significant hepatic lipids that were increased by ethanol exposure included fatty acids and triglycerides, whereas phosphatidylcholine (PC) decreased. However, both free fatty acids and PC decreased in the plasma. In liver lipids unsaturation of fatty acyl chains increased, contrary to plasma, where it decreased. Our studies confirm that over-accumulation of lipids in ethanol-induced liver steatosis accompanied by mild inflammation on long duration of ethanol exposure. Identified metabolic profile using NMR lipidomics could be further explored to establish biomarker signatures representing the etiopathogenesis, progression and/or severity of ALD. - Highlights: > Long term exposure to ethanol was studied. > A nuclear magnetic resonance (NMR) spectroscopy based lipidomic approach was used. > We examined the clustering pattern of the NMR data with principal component analysis. > NMR data were compared with histology and immunohistochemistry data. > Biochemical parameters were compared with the observed NMR lipid data.

  4. Iron supplementation at high altitudes induces inflammation and oxidative injury to lung tissues in rats

    SciTech Connect (OSTI)

    Salama, Samir A.; Omar, Hany A.; Maghrabi, Ibrahim A.; AlSaeed, Mohammed S.; EL-Tarras, Adel E.

    2014-01-01

    Exposure to high altitudes is associated with hypoxia and increased vulnerability to oxidative stress. Polycythemia (increased number of circulating erythrocytes) develops to compensate the high altitude associated hypoxia. Iron supplementation is, thus, recommended to meet the demand for the physiological polycythemia. Iron is a major player in redox reactions and may exacerbate the high altitudes-associated oxidative stress. The aim of this study was to explore the potential iron-induced oxidative lung tissue injury in rats at high altitudes (6000 ft above the sea level). Iron supplementation (2 mg elemental iron/kg, once daily for 15 days) induced histopathological changes to lung tissues that include severe congestion, dilatation of the blood vessels, emphysema in the air alveoli, and peribronchial inflammatory cell infiltration. The levels of pro-inflammatory cytokines (IL-1?, IL-6, and TNF-?), lipid peroxidation product and protein carbonyl content in lung tissues were significantly elevated. Moreover, the levels of reduced glutathione and total antioxidant capacity were significantly reduced. Co-administration of trolox, a water soluble vitamin E analog (25 mg/kg, once daily for the last 7 days of iron supplementation), alleviated the lung histological impairments, significantly decreased the pro-inflammatory cytokines, and restored the oxidative stress markers. Together, our findings indicate that iron supplementation at high altitudes induces lung tissue injury in rats. This injury could be mediated through excessive production of reactive oxygen species and induction of inflammatory responses. The study highlights the tissue injury induced by iron supplementation at high altitudes and suggests the co-administration of antioxidants such as trolox as protective measures. - Highlights: Iron supplementation at high altitudes induced lung histological changes in rats. Iron induced oxidative stress in lung tissues of rats at high altitudes. Iron increased the levels of IL-1?, IL-6 and TNF-? in lung tissues at high altitudes. Trolox alleviated the iron-induced histological and biochemical changes to the lungs.

  5. Effects of Love Canal soil extracts on maternal health and fetal development in rats

    SciTech Connect (OSTI)

    Silkworth, J.B.; Tumasonis, C.; Briggs, R.G.; Narang, A.S.; Narang, R.S.; Rej, R.; Stein, V.; McMartin, D.N.; Kaminsky, L.S.

    1986-10-01

    The effects of a solvent extract of the surface soil of the Love Canal chemical dump site, Niagara Falls, New York, and of a natural extract, or leachate, which is drained from the canal for treatment, on the maternal health and fetal development were determined in rats. The solvent extract, which was contaminated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (2, 3,7,8-TCDD) at 170 ppb and numerous other chlorinated organic compounds with the primary identified components being the isomers of benzenehexachloride (BHC), was dissolved in corn oil and administered by gavage to pregnant rats at 0,25,75, or 150 mg crude extract/kg/day on Days 6-15 of gestation. A 67% mortality was observed at the highest dose. The rats were sacrificed on Day 20. Dose-related increases in relative liver weight accompanied by hepatocyte hypertrophy were observed at all dose levels. Fetal birthweight was decreased at 75 and 150 mg extract/kg/day. No major treatment-related soft tissue or skeletal malformations, except for delayed ossification, were observed. Based on literature values for BHC, all of the observed toxicity could be accounted for by the BHC contaminants of the extract. The crude organic phase of the leachate was administered to pregnant rats at 0,10,100, or 250 mg/kg/day as described above. Maternal weight gain decreased at 100 and 250 mg/kg/day, accompanied by 5 and 14% maternal mortality, and 1 and 3 dead fetuses, respectively. Early resorptions and the percentage of dead implants increased whereas fetal birthweights were decreased at 250 mg/kg/day. No major treatment-related soft tissue or skeletal malformations, except for delayed ossification, were observed. The primary components of the complex leachate by mass were tetrachloroethanes; however, 2,3,7,8-TCDD, which was present at 3 ppm, probably accounted for all the observed toxicity.

  6. Thyroid organotypic rat and human cultures used to investigate drug effects on thyroid function, hormone synthesis and release pathways

    SciTech Connect (OSTI)

    Vickers, Alison E.M.; Heale, Jason; Sinclair, John R.; Morris, Stephen; Rowe, Josh M.; Fisher, Robyn L.

    2012-04-01

    Drug induced thyroid effects were evaluated in organotypic models utilizing either a rat thyroid lobe or human thyroid slices to compare rodent and human response. An inhibition of thyroid peroxidase (TPO) function led to a perturbation in the expression of key genes in thyroid hormone synthesis and release pathways. The clinically used thiourea drugs, methimazole (MMI) and 6-n-propyl-2-thioruacil (PTU), were used to evaluate thyroid drug response in these models. Inhibition of TPO occurred early as shown in rat thyroid lobes (2 h) and was sustained in both rat (2448 h) and human (24 h) with ? 10 ?M MMI. Thyroid from rats treated with single doses of MMI (301000 mg/kg) exhibited sustained TPO inhibition at 48 h. The MMI in vivo thyroid concentrations were comparable to the culture concentrations (? 1584 ?M), thus demonstrating a close correlation between in vivo and ex vivo thyroid effects. A compensatory response to TPO inhibition was demonstrated in the rat thyroid lobe with significant up-regulation of genes involved in the pathway of thyroid hormone synthesis (Tpo, Dio1, Slc5a5, Tg, Tshr) and the megalin release pathway (Lrp2) by 24 h with MMI (? 10 ?M) and PTU (100 ?M). Similarly, thyroid from the rat in vivo study exhibited an up-regulation of Dio1, Slc5a5, Lrp2, and Tshr. In human thyroid slices, there were few gene expression changes (Slc5a5, ? 2-fold) and only at higher MMI concentrations (? 1500 ?M, 24 h). Extended exposure (48 h) resulted in up-regulation of Tpo, Dio1 and Lrp2, along with Slc5a5 and Tshr. In summary, TPO was inhibited by similar MMI concentrations in rat and human tissue, however an increased sensitivity to drug treatment in rat is indicated by the up-regulation of thyroid hormone synthesis and release gene pathways at concentrations found not to affect human tissue. -- Highlights: ? Novel model of rat thyroid or human thyroid slices to evaluate pathways of injury. ? TPO inhibition by MMI or PTU altered hormone synthesis and release genes. ? Rat thyroid was more sensitive to the drug effects than human tissue.

  7. Association of brominated proteins and changes in protein expression in the rat kidney with subcarcinogenic to carcinogenic doses of bromate

    SciTech Connect (OSTI)

    Kolisetty, Narendrababu [Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602 (United States); Bull, Richard J. [MoBull Consulting, Richland, WA 99352 (United States); Muralidhara, Srinivasa; Costyn, Leah J. [Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602 (United States); Delker, Don A. [School of Medicine, University of Utah, Salt Lake City, UT 84132 (United States); Guo, Zhongxian [Water Quality Office, Public Utilities Board, 608576 (Singapore); Cotruvo, Joseph A. [Joseph Cotruvo and Associates, LLC, Washington, DC 20016 (United States); Fisher, Jeffrey W. [National Center for Toxicological Research, FDA, Jefferson, AR 72079 (United States); Cummings, Brian S., E-mail: bsc@rx.uga.edu [Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602 (United States)

    2013-10-15

    The water disinfection byproduct bromate (BrO{sub 3}{sup ?}) produces cytotoxic and carcinogenic effects in rat kidneys. Our previous studies demonstrated that BrO{sub 3}{sup ?} caused sex-dependent differences in renal gene and protein expression in rats and the elimination of brominated organic carbon in their urine. The present study examined changes in renal cell apoptosis and protein expression in male and female F344 rats treated with BrO{sub 3}{sup ?} and associated these changes with accumulation of 3-bromotyrosine (3-BT)-modified proteins. Rats were treated with 0, 11.5, 46 and 308 mg/L BrO{sub 3}{sup ?} in drinking water for 28 days and renal sections were prepared and examined for apoptosis (TUNEL-staining), 8-oxo-deoxyguanosine (8-oxoG), 3-BT, osteopontin, Kim-1, clusterin, and p-21 expression. TUNEL-staining in renal proximal tubules increased in a dose-related manner beginning at 11.5 mg BrO{sub 3}{sup ?}/L in female rats and 46 mg/L in males. Increased 8-oxoG staining was observed at doses as low as 46 mg/L. Osteopontin expression also increased in a dose-related manner after treatment with 46 mg/L, in males only. In contrast, Kim-1 expression increased in a dose-related manner in both sexes, although to a greater extent in females at the highest dose. Clusterin and p21 expression also increased in a dose-related manner in both sexes. The expression of 3-BT-modified proteins only increased in male rats, following a pattern previously reported for accumulation of ?-2{sub u}-globulin. Increases in apoptosis in renal proximal tubules of male and female rats at the lowest doses suggest a common mode of action for renal carcinogenesis for the two sexes that is independent of ?-2{sub u}-globulin nephropathy. - Highlights: Bromate induced nephrotoxicity in both male and female rats by similar mechanisms. Apoptosis was seen in both male and female rats at the lowest doses tested. Bromate-induced apoptosis correlated to 8-oxo-deoxyguanosine formation. Bromate increased the level of 3-bromotyrosine-modified proteins in male rats only. These data identify possible novel mechanisms for bromate-induced nephrotoxicity.

  8. Physiological effects following administration of Citrus aurantium for 28 days in rats

    SciTech Connect (OSTI)

    Hansen, Deborah K.; Pellicore, Linda S.

    2012-06-15

    Background: Since ephedra-containing dietary supplements were banned from the US market, manufacturers changed their formulations by eliminating ephedra and replacing with other botanicals, including Citrus aurantium, or bitter orange. Bitter orange contains, among other compounds, synephrine, a chemical that is chemically similar to ephedrine. Since ephedrine may have cardiovascular effects, the goal of this study was to investigate the cardiovascular effects of various doses of bitter orange extract and pure synephrine in rats. Method: Female SpragueDawley rats were dosed daily by gavage for 28 days with synephrine from two different extracts. One extract contained 6% synephrine, and the other extract contained 95% synephrine. Doses were 10 or 50 mg synephrine/kg body weight from each extract. Additionally, caffeine was added to these doses, since many dietary supplements also contain caffeine. Telemetry was utilized to monitor heart rate, blood pressure, body temperature and QT interval in all rats. Results and conclusion: Synephrine, either as the bitter orange extract or as pure synephrine, increased heart rate and blood pressure. Animals treated with 95% synephrine showed minimal effects on heart rate and blood pressure; more significant effects were observed with the bitter orange extract suggesting that other components in the botanical can alter these physiological parameters. The increases in heart rate and blood pressure were more pronounced when caffeine was added. None of the treatments affected uncorrected QT interval in the absence of caffeine.

  9. Life-threatening interaction between the root extract of Pueraria lobata and methotrexate in rats

    SciTech Connect (OSTI)

    Chiang, H.-M.; Fang, S.-H.; Wen, K.-C.; Hsiu, S.-L.; Tsai, Shang-Yuan; Hou, Y.-C.; Chi, Y.-C.; Lee Chao, Pei-Dawn . E-mail: pdlee@mail.cmu.edu.tw

    2005-12-15

    Isoflavone supplements are nowadays widely used as alternative for hormone replacement therapy. However, the safety remains unanswered. This study attempted to investigate the effect of Pueraria lobata root decoction (PLRD), an isoflavone-rich herb, on the pharmacokinetics of methotrexate (MTX), a bicarboxylate antimetabolite with narrow therapeutic window. Rats were orally and intravenously given methotrexate alone and coadministered with PLRD. Blood samples were withdrawn via cardiopuncture at specific time points after drug administration. Serum methotrexate concentrations were assayed by specific monoclonal fluorescence polarization immunoassay method. Pharmacokinetic parameters were calculated using noncompartment model of WINNONLIN for both oral and intravenous data of MTX. Our results showed that coadministration of 4.0 g/kg and 2.0 g/kg of PLRD significantly increased the AUC{sub 0-t} by 207.8% and 127.9%, prolonged the mean residence time (MRT) by 237.8 and 155.2%, respectively, finally resulted in surprisingly high mortalities of 57.1% and 14.3% in rats. When MTX was given intravenously, the coadministration of PLRD at 4.0 g/kg significantly increased the half-life by 53.9% and decreased the clearance by 47.9%. In conclusion, the coadministration of PLRD significantly decreased the elimination and resulted in markedly increased exposure of MTX in rats.

  10. Application of metabonomics on an experimental model of fibrosis and cirrhosis induced by thioacetamide in rats

    SciTech Connect (OSTI)

    Constantinou, Maria A.; Theocharis, Stamatios E.; Mikros, Emmanuel . E-mail: mikros@pharm.uoa.gr

    2007-01-01

    Metabonomics has already been used to discriminate different pathological states in biological fields. The metabolic profiles of chronic experimental fibrosis and cirrhosis induction in rats were investigated using {sup 1}H NMR spectroscopy of liver extracts and serum combined with pattern recognition techniques. Rats were continuously administered with thioacetamide (TAA) in the drinking water (300 mg TAA/L), and sacrificed on 1st, 2nd, and 3rd month of treatment. {sup 1}H NMR spectra of aqueous and lipid liver extracts, together with serum were subjected to Principal Component Analysis (PCA). Liver portions were also subjected to histopathological examination and biochemical determination of malondialdehyde (MDA). Liver fibrosis and cirrhosis were progressively induced in TAA-treated rats, verified by the histopathological examination and the alterations of MDA levels. TAA administration revealed a number of changes in the {sup 1}H NMR spectra compared to control samples. The performance of PCA in liver extracts and serum, discriminated the control samples from the fibrotic and cirrhotic ones. Metabolic alterations revealed in NMR spectra during experimental liver fibrosis and cirrhosis induction, characterize the stage of fibrosis and could be illustrated by subsequent PCA of the spectra. Additionally, the PCA plots of the serum samples presented marked clustering during fibrosis progression and could be extended in clinical diagnosis for the management of cirrhotic patients.

  11. A STUDY OF FISCHER 344 RATS EXPOSED TO SILICA DUST FOR SIX MONTHS AT CONCENTRATIONS OF 0, 2, 10 OR 20 MG / M3.

    SciTech Connect (OSTI)

    KUTZMAN,R.S.

    1984-02-01

    The major objective of this study was to relate the results of a series of functional tests to the compositional and structural alterations in the rat lung induced by subchronic exposure to silica dust. Fischer-344 rats were exposed for 6 hours/day, 5 days/week for 6 months to either 0, 2, 10, or 20 mg SiO{sub 2}/m{sup 3}. The general appearance of the exposed rats was not different from that of the controls. Interestingly, female rats exposed to silica dust, at all tested concentrations, gained more weight than the controls. The lung weight and the lung-to-body weight ratio was greater in the male rats exposed to the highest concentration of silica dust.

  12. Estrous cycle affects the neurochemical and neurobehavioral profile of carvacrol-treated female rats

    SciTech Connect (OSTI)

    Trabace, L.; Zotti, M.; Morgese, M.G.; Tucci, P.; Colaianna, M.; Schiavone, S.; Avato, P.; Cuomo, V.

    2011-09-01

    Carvacrol is the major constituent of essential oils from aromatic plants. It showed antimicrobial, anticancer and antioxidant properties. Although it was approved for food use and included in the chemical flavorings list, no indication on its safety has been estimated. Since the use of plant extracts is relatively high among women, aim of this study was to evaluate carvacrol effects on female physiology and endocrine profiles by using female rats in proestrus and diestrus phases. Serotonin and metabolite tissue content in prefrontal cortex and nucleus accumbens, after carvacrol administration (0.15 and 0.45 g/kg p.o.), was measured. Drug effects in behavioral tests for alterations in motor activity, depression, anxiety-related behaviors and endocrine alterations were also investigated. While in proestrus carvacrol reduced serotonin and metabolite levels in both brain areas, no effects were observed in diestrus phase. Only in proestrus phase, carvacrol induced a depressive-like behavior in forced swimming test, without accompanying changes in ambulation. The improvement of performance in FST after subchronic treatment with fluoxetine (20 mg/kg) suggested a specific involvement of serotonergic system. No differences were found across the groups with regard to self-grooming behavior. Moreover, in proestrus phase, carvacrol reduced only estradiol levels without binding hypothalamic estradiol receptors. Our study showed an estrous-stage specific effect of carvacrol on depressive behaviors and endocrine parameters, involving serotonergic system. Given the wide carvacrol use not only as feed additive, but also as cosmetic essence and herbal remedy, our results suggest that an accurate investigation on the effects of its chronic exposure is warranted. - Highlights: > Carvacrol induced a depressive-like phenotype in rats, depending on ovarian cyclicity. > Carvacrol selectively reduced serotonin content in female rats in proestrus phase. > Carvacrol reduced serotonin levels in areas belonging to the emotional circuit. > Carvacrol reduced plasma estradiol levels only during the proestrus phase.

  13. RatBot: anti-enumeration peer-to-peer botnets

    SciTech Connect (OSTI)

    Yan, Guanhua; Eidenbenz, Stephan; Chen, Songqing

    2010-01-01

    Botnets have emerged as one of the most severe cyber threats in recent years. To obtain high resilience against a single point of failure, the new generation of botnets have adopted the peer-to-peer (P2P) structure. One critical question regarding these P2P botnets is: how big are they indeed? To address this question, researchers have proposed both actively crawling and passively monitoring methods to enumerate existing P2P botnets. In this work, we go further to explore the potential strategies that botnets may have to obfuscate their true sizes. Towards this end, this paper introduces RatBot, a P2P botnet that applies some statistical techniques to defeat existing P2P botnet enumeration methods. The key ideas of RatBot are two-fold: (1) there exist a fraction of bots that are indistinguishable from their fake identities, which are spoofing IP addresses they use to hide themselves; (2) we use a heavy-tailed distribution to generate the number of fake identities for each of these bots so that the sum of observed fake identities converges only slowly and thus has high variation. We use large-scale high-fidelity simulation to quantify the estimation errors under diverse settings, and the results show that a naive enumeration technique can overestimate the sizes of P2P botnets by one order of magnitude. We believe that our work reveals new challenges of accurately estimating the sizes of P2P botnets, and hope that it will raise the awareness of security practitioners with these challenges. We further suggest a few countermeasures that can potentially defeat RatBot's anti-enumeration scheme.

  14. Daily treatment with {alpha}-naphthoflavone enhances follicular growth and ovulation rate in the rat

    SciTech Connect (OSTI)

    Barreiro, Karina A.; Di Yorio, Maria P.; Artillo-Guida, Romina D.; Paz, Dante A.; Faletti, Alicia G.

    2011-04-01

    The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovarian toxicity of many environmental contaminants, which are AhR ligands. However, the role of AhR in the ovarian function is unknown. Therefore, the aim of this work was to study the action of {alpha}-naphthoflavone ({alpha}NF), known to be an AhR antagonist, on both follicular growth and ovulation. Immature Sprague-Dawley rats were daily injected intraperitoneally with {alpha}NF (0.1-80 mg/kg) or vehicle for 12 days, and primed with gonadotrophins (eCG/hCG) to induce follicular growth and ovulation. Ovaries were obtained 20 h after hCG administration. By means of immunohistochemistry, we found that the numbers of primordial, primary and antral follicles were increased in rats treated with 80 mg/kg {alpha}NF and that there were no differences with other doses. Likewise, the ovarian weight and the ovulation rate, measured by both number of oocytes within oviducts and corpora lutea in ovarian sections, were increased when the rats received either 1 or 10 mg/kg daily. Although further studies are necessary to know the mechanism of action of {alpha}NF, it is possible that the different ovarian processes can be differentially responsive to the presence of different levels of {alpha}NF, and that the same or different endogenous AhR ligands can be involved in these ovarian processes in a cell type-dependent manner.

  15. Subacute effects of hexabromocyclododecane (HBCD) on hepatic gene expression profiles in rats

    SciTech Connect (OSTI)

    Canton, Rocio F. [Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80.177, NL-3508 TD, Utrecht (Netherlands)], E-mail: rfcanton@gmail.com; Peijnenburg, Ad A.C.M.; Hoogenboom, Ron L.A.P. [RIKILT Institute of Food Safety, Wageningen University and Research Center, P.O. Box 230, 6700 AE Wageningen (Netherlands); Piersma, Aldert H.; Ven, Leo T.M. van der [National Institute of Public Health and the Environment (RIVM), Laboratory for Heath Protection Research, P.O. Box 1, 3720 BA Bilthoven (Netherlands); Berg, Martin van den [Institute for Risk Assessment Sciences (IRAS), Utrecht University, P.O. Box 80.177, NL-3508 TD, Utrecht (Netherlands); Heneweer, Marjoke [RIKILT Institute of Food Safety, Wageningen University and Research Center, P.O. Box 230, 6700 AE Wageningen (Netherlands)

    2008-09-01

    Hexabromoyclododecane (HBCD), used as flame retardant (FR) mainly in textile industry and in polystyrene foam manufacture, has been identified as a contaminant at levels comparable to other brominated FRs (BFRs). HBCD levels in biota are increasing slowly and seem to reflect the local market demand. The toxicological database of HBCD is too limited to perform at present a solid risk assessment, combining data from exposure and effect studies. In order to fill in some gaps, a 28-day HBCD repeated dose study (OECD407) was done in Wistar rats. In the present work liver tissues from these animals were used for gene expression profile analysis. Results show clear gender specificity with females having a higher number of regulated genes and therefore being more sensitive to HBCD than males. Several specific pathways were found to be affected by HBCD exposure, like PPAR-mediated regulation of lipid metabolism, triacylglycerol metabolism, cholesterol biosynthesis, and phase I and II pathways. These results were corroborated with quantitative RT-PCR analysis. Cholesterol biosynthesis and lipid metabolism were especially down-regulated in females. Genes involved in phase I and II metabolism were up-regulated predominantly in males, which could explain the observed lower HBCD hepatic disposition in male rats in this 28-day study. These sex-specific differences in gene expression profiles could also underlie sex-specific differences in toxicity (e.g. decreased thyroid hormone or increased serum cholesterol levels). To our knowledge, this is the fist study that describes the changes in rat hepatic gene profiles caused by this commonly used flame retardant.

  16. Effect of intranasal manganese administration on neurotransmission and spatial learning in rats

    SciTech Connect (OSTI)

    Blecharz-Klin, Kamilla; Piechal, Agnieszka; Joniec-Maciejak, Ilona; Pyrzanowska, Justyna; Widy-Tyszkiewicz, Ewa

    2012-11-15

    The effect of intranasal manganese chloride (MnCl{sub 2}4H{sub 2}O) exposure on spatial learning, memory and motor activity was estimated in Morris water maze task in adult rats. Three-month-old male Wistar rats received for 2 weeks MnCl{sub 2}4H{sub 2}O at two doses the following: 0.2 mg/kg b.w. (Mn0.2) or 0.8 mg/kg b.w. (Mn0.8) per day. Control (Con) and manganese-exposed groups were observed for behavioral performance and learning in water maze. ANOVA for repeated measurements did not show any significant differences in acquisition in the water maze between the groups. However, the results of the probe trial on day 5, exhibited spatial memory deficits following manganese treatment. After completion of the behavioral experiment, the regional brain concentrations of neurotransmitters and their metabolites were determined via HPLC in selected brain regions, i.e. prefrontal cortex, hippocampus and striatum. ANOVA demonstrated significant differences in the content of monoamines and metabolites between the treatment groups compared to the controls. Negative correlations between platform crossings on the previous platform position in Southeast (SE) quadrant during the probe trial and neurotransmitter turnover suggest that impairment of spatial memory and cognitive performance after manganese (Mn) treatment is associated with modulation of the serotonergic, noradrenergic and dopaminergic neurotransmission in the brain. These findings show that intranasally applied Mn can impair spatial memory with significant changes in the tissue level and metabolism of monoamines in several brain regions. -- Highlights: ? Intranasal exposure to manganese in rats impairs spatial memory in the water maze. ? Regional changes in levels of neurotransmitters in the brain have been identified. ? Cognitive disorder correlates with modulation of 5-HT, NA and DA neurotransmission.

  17. Response of rat brain protein synthesis to ethanol and sodium barbital

    SciTech Connect (OSTI)

    Tewari, S.; Greenberg, S.A.; Do, K.; Grey, P.A.

    1987-01-01

    Central nervous system (CNS) depressants such as ethanol and barbiturates under acute or chronic conditions can induce changes in rat brain protein synthesis. While these data demonstrate the individual effects of drugs on protein synthesis, the response of brain protein synthesis to alcohol-drug interactions is not known. The goal of the present study was to determine the individual and combined effects of ethanol and sodium barbital on brain protein synthesis and gain an understanding of the mechanisms by which these alterations in protein synthesis are produced. Specifically, the in vivo and in vitro effects of sodium barbital (one class of barbiturates which is not metabolized by the hepatic tissue) were examined on brain protein synthesis in rats made physically dependent upon ethanol. Using cell free brain polysomal systems isolated from Control, Ethanol and 24 h Ethanol Withdrawn rats, data show that sodium barbital, when intubated intragastrically, inhibited the time dependent incorporation of /sup 14/C) leucine into protein by all three groups of ribosomes. Under these conditions, the Ethanol Withdrawn group displayed the largest inhibition of the /sup 14/C) leucine incorporation into protein when compared to the Control and Ethanol groups. In addition, sodium barbital when added at various concentrations in vitro to the incubation medium inhibited the incorporation of /sup 14/C) leucine into protein by Control and Ethanol polysomes. The inhibitory effects were also obtained following preincubation of ribosomes in the presence of barbital but not cycloheximide. Data suggest that brain protein synthesis, specifically brain polysomes, through interaction with ethanol or barbital are involved in the functional development of tolerance. These interactions may occur through proteins or polypeptide chains or alterations in messenger RNA components associated with the ribosomal units.

  18. Differential metabolism of 4-hydroxynonenal in liver, lung and brain of mice and rats

    SciTech Connect (OSTI)

    Zheng, Ruijin; Dragomir, Ana-Cristina; Mishin, Vladimir; Richardson, Jason R.; Heck, Diane E.; Laskin, Debra L.; Laskin, Jeffrey D.

    2014-08-15

    The lipid peroxidation end-product 4-hydroxynonenal (4-HNE) is generated in tissues during oxidative stress. As a reactive aldehyde, it forms Michael adducts with nucleophiles, a process that disrupts cellular functioning. Liver, lung and brain are highly sensitive to xenobiotic-induced oxidative stress and readily generate 4-HNE. In the present studies, we compared 4-HNE metabolism in these tissues, a process that protects against tissue injury. 4-HNE was degraded slowly in total homogenates and S9 fractions of mouse liver, lung and brain. In liver, but not lung or brain, NAD(P)+ and NAD(P)H markedly stimulated 4-HNE metabolism. Similar results were observed in rat S9 fractions from these tissues. In liver, lung and brain S9 fractions, 4-HNE formed protein adducts. When NADH was used to stimulate 4-HNE metabolism, the formation of protein adducts was suppressed in liver, but not lung or brain. In both mouse and rat tissues, 4-HNE was also metabolized by glutathione S-transferases. The greatest activity was noted in livers of mice and in lungs of rats; relatively low glutathione S-transferase activity was detected in brain. In mouse hepatocytes, 4-HNE was rapidly taken up and metabolized. Simultaneously, 4-HNE-protein adducts were formed, suggesting that 4-HNE metabolism in intact cells does not prevent protein modifications. These data demonstrate that, in contrast to liver, lung and brain have a limited capacity to metabolize 4-HNE. The persistence of 4-HNE in these tissues may increase the likelihood of tissue injury during oxidative stress. - Highlights: • Lipid peroxidation generates 4-hydroxynonenal, a highly reactive aldehyde. • Rodent liver, but not lung or brain, is efficient in degrading 4-hydroxynonenal. • 4-hydroxynonenal persists in tissues with low metabolism, causing tissue damage.

  19. Size and charge effects of dopant M on the unit-cell parameters of monoclinic zirconia solid solutions Zr{sub 0.98}M{sub 0.02}O{sub 2{minus}{delta}} (M = Ce, La, Nd, Sm, Y, Er, Yb, Sc, Mg, Ca)

    SciTech Connect (OSTI)

    Yashima, Masatomo; Kakihana, Masato; Yoshimura, Masahiro; Hirose, Teruo; Suzuki, Yasuo

    1997-01-01

    The crystal structure of monoclinic phase [P2{sub 1}/c, Z = 4] has been refined by the Rietveld analysis of X-ray powder diffraction data to study the size and charge effects of dopant M{sup n+} on the unit-cell parameters of monoclinic ZrO{sub 2}-2 mol% MO{sub n/2} solid solutions (n = 4 for M = Ce; n = 3 for M = La, Nd, Sm, Y, Er, Yb, Sc; and n = 2 for M = Mg and Ca). For trivalent dopant (n = 3), the unit-cell parameters a{sub m}, b{sub m}, c{sub m} and unit-cell volume increase and {beta}{sub m} decreases with an increase of dopant size. Unit-cell volume increases with increasing of dopant charge n.

  20. COMPARATIVE COMPUTATIONAL MODELING OF AIRFLOWS AND VAPOR DOSIMETY IN THE RESPIRATORY TRACTS OF RAT, MONKEY, AND HUMAN

    SciTech Connect (OSTI)

    Corley, Richard A.; Kabilan, Senthil; Kuprat, Andrew P.; Carson, James P.; Minard, Kevin R.; Jacob, Rick E.; Timchalk, Charles; Glenny, Robb W.; Pipavath, Sudhaker; Cox, Timothy C.; Wallis, Chris; Larson, Richard; Fanucchi, M.; Postlewait, Ed; Einstein, Daniel R.

    2012-07-01

    Coupling computational fluid dynamics (CFD) with physiologically based pharmacokinetic (PBPK) models is useful for predicting site-specific dosimetry of airborne materials in the respiratory tract and elucidating the importance of species differences in anatomy, physiology, and breathing patterns. Historically, these models were limited to discrete regions of the respiratory system. CFD/PBPK models have now been developed for the rat, monkey, and human that encompass airways from the nose or mouth to the lung. A PBPK model previously developed to describe acrolein uptake in nasal tissues was adapted to the extended airway models as an example application. Model parameters for each anatomic region were obtained from the literature, measured directly, or estimated from published data. Airflow and site-specific acrolein uptake patterns were determined under steadystate inhalation conditions to provide direct comparisons with prior data and nasalonly simulations. Results confirmed that regional uptake was dependent upon airflow rates and acrolein concentrations with nasal extraction efficiencies predicted to be greatest in the rat, followed by the monkey, then the human. For human oral-breathing simulations, acrolein uptake rates in oropharyngeal and laryngeal tissues were comparable to nasal tissues following nasal breathing under the same exposure conditions. For both breathing modes, higher uptake rates were predicted for lower tracheo-bronchial tissues of humans than either the rat or monkey. These extended airway models provide a unique foundation for comparing dosimetry across a significantly more extensive range of conducting airways in the rat, monkey, and human than prior CFD models.

  1. Bromocriptine increased operant responding for high fat food but decreased chow intake in both obesity-prone and resistant rats

    SciTech Connect (OSTI)

    Thanos, P.K.; Wang, G.; Thanos, P.K.; Cho, J. Kim, R.; Michaelides, M.; Primeaux, S.; Bray, G.; Wang, G.-J.; Volkow, N.D.

    2010-10-27

    Dopamine (DA) and DAD{sub 2} receptors (D2R) have been implicated in obesity and are thought to be involved in the rewarding properties of food. Osborne-Mendel (OM) rats are susceptible to diet induced obesity (DIO) while S5B/P (S5B) rats are resistant when given a high-fat diet. Here we hypothesized that the two strains would differ in high-fat food self-administration (FSA) and that the D2R agonist bromocriptine (BC) would differently affect their behavior. Ad-libitum fed OM and S5B/P rats were tested in a FSA operant chamber and were trained to lever press for high-fat food pellets under a fixed-ratio (FR1) and a progressive ratio (PR) schedule. After sixteen days of PR sessions, rats were treated with three different doses of BC (1, 10 and 20 mg/kg). No significant differences were found between the two strains in the number of active lever presses. BC treatment (10 mg/kg and 20 mg/kg) increased the number of active lever presses (10 mg/kg having the strongest effect) whereas it decreased rat chow intake in the home cage with equivalent effects in both strains. These effects were not observed on the day of BC administration but on the day following its administration. Our results suggest that these two strains have similar motivation for procuring high fat food using this paradigm. BC increased operant responding for high-fat pellets but decreased chow intake in both strains, suggesting that D2R stimulation may have enhanced the motivational drive to procure the fatty food while correspondingly decreasing the intake of regular food. These findings suggest that susceptibility to dietary obesity (prior to the onset of obesity) may not affect operant motivation for a palatable high fat food and that differential susceptibility to obesity may be related to differential sensitivity to D2R stimulation.

  2. Application of computational fluid dynamics to regional dosimetry of inhaled chemicals in the upper respiratory tract of the rat

    SciTech Connect (OSTI)

    Kimbell, J.S.; Gross, E.A.; Joyner, D.R.; Godo, M.N.; Morgan, K.T. (Chemical Industry Institute of Toxicology, Research Triangle Park, NC (United States))

    1993-08-01

    For certain inhaled air pollutants, such as reactive, water soluble gases, the distribution of nasal lesions observed in F344 rats may be closely related to regional gas uptake patterns in the nose. These uptake patterns can be influenced by the currents of air flowing through the upper respiratory tract during the breathing cycle. Since data on respiratory tract lesions in F344 rats are extrapolated to humans to make predictions of risk to human health, a better understanding of the factors affecting these responses is needed. To assess potential effects of nasal airflow on lesion location and severity, a methodology was developed for creation of computer simulations of steady-state airflow and gas transport using a three-dimensional finite element grid reconstructed from serial step-sections of the nasal passages of a male F344 rat. Simulations on a supercomputer used the computational fluid dynamics package FIDAP (FDI, Evanston, IL). Distinct streams of bulk flow evident in the simulations matched inspiratory streams reported for the F344 rat. Moreover, simulated regional flow velocities matched measured velocities in concurrent laboratory experiments with a hollow nasal mold. Computer-predicted flows were used in simulations of gas transport to nasal passage walls, with formaldehyde as a test case. Results from the uptake simulations were compared with the reported distribution of formaldehyde-induced nasal lesions observed in the F344 rat, and indicated that airflow-driven uptake patterns probably play an important role in determining the location of certain nasal lesions induced by formaldehyde. This work demonstrated the feasibility of applying computational fluid dynamics to airflow-driven dosimetry of inhaled chemicals in the upper respiratory tract.

  3. In Situ Casting and Imaging of the Rat Airway Tree for Accurate 3D Reconstruction

    SciTech Connect (OSTI)

    Jacob, Rick E.; Colby, Sean M.; Kabilan, Senthil; Einstein, Daniel R.; Carson, James P.

    2013-08-01

    The use of anatomically accurate, animal-specific airway geometries is important for understanding and modeling the physiology of the respiratory system. One approach for acquiring detailed airway architecture is to create a bronchial cast of the conducting airways. However, typical casting procedures either do not faithfully preserve the in vivo branching angles, or produce rigid casts that when removed for imaging are fragile and thus easily damaged. We address these problems by creating an in situ bronchial cast of the conducting airways in rats that can be subsequently imaged in situ using 3D micro-CT imaging. We also demonstrate that deformations in airway branch angles resulting from the casting procedure are small, and that these angle deformations can be reversed through an interactive adjustment of the segmented cast geometry. Animal work was approved by the Institutional Animal Care and Use Committee of Pacific Northwest National Laboratory.

  4. Laser speckle-imaging of blood microcirculation in the brain cortex of laboratory rats in stress

    SciTech Connect (OSTI)

    Vilensky, M A; Semyachkina-Glushkovskaya, Oxana V; Timoshina, P A; Kuznetsova, Jana V; Semyachkin-Glushkovskii, I A; Agafonov, Dmitry N; Tuchin, Valerii V

    2012-06-30

    The results of experimental approbation of the method of laser full-field speckle-imaging for monitoring the changes in blood microcirculation state of the brain cortex of laboratory rats under the conditions of developing stroke and administration of vasodilating and vasoconstrictive agents are presented. The studies aimed at the choice of the optimal conditions of speckle-image formation and recording were performed and the software implementing an adaptive algorithm for processing the data of measurements was created. The transfer of laser radiation to the probed region of the biotissue was implemented by means of a silica-polymer optical fibre. The problems and prospects of speckle-imaging of cerebral microcirculation of blood in laboratory and clinical conditions are discussed.

  5. CPEB1 modulates lipopolysaccharide-mediated iNOS induction in rat primary astrocytes

    SciTech Connect (OSTI)

    Kim, Ki Chan; Hyun Joo, So; Shin, Chan Young

    2011-06-17

    Highlights: {yields} Expression and phosphorylation of CPEB1 is increased by LPS stimulation in rat primary astrocytes. {yields} JNK regulates expression and phosphorylation of CPEB1 in reactive astrocytes. {yields} Down-regulation of CPEB1 using siRNA inhibits oxidative stress and iNOS induction by LPS stimulation. {yields} CPEB1 may play an important role in regulating inflammatory responses in reactive astrocytes induced by LPS. -- Abstract: Upon CNS damage, astrocytes undergo a series of biological changes including increased proliferation, production of inflammatory mediators and morphological changes, in a response collectively called reactive gliosis. This process is an essential part of the brains response to injury, yet much is unknown about the molecular mechanism(s) that induce these changes. In this study, we investigated the role of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in the regulation of inflammatory responses in a model of reactive gliosis, lipopolysaccharide-stimulated astrocytes. CPEB1 is an mRNA-binding protein recently shown to be expressed in astrocytes that may play a role in astrocytes migration. After LPS stimulation, the expression and phosphorylation of CPEB1 was increased in rat primary astrocytes in a JNK-dependent process. siRNA-induced knockdown of CPEB1 expression inhibited the LPS-induced up-regulation of iNOS as well as NO and ROS production, a hallmark of immunological activation of astrocytes. The results from the study suggest that CPEB1 is actively involved in the regulation of inflammatory responses in astrocytes, which might provide new insights into the regulatory mechanism after brain injury.

  6. Evaluation of the pharmacokinetics and cardiotoxicity of doxorubicin in rat receiving nilotinib

    SciTech Connect (OSTI)

    Zhou, Zhi-yong; Wan, Li-li; Yang, Quan-jun; Han, Yong-long; Li, Yan; Yu, Qi; Guo, Cheng; Li, Xiao

    2013-10-01

    Doxorubicin (DOX) is a potent chemotherapy drug with a narrow therapeutic window. Nilotinib, a small-molecule Bcr-Abl tyrosine kinase inhibitor, was reported to reverse multidrug resistance (MDR) mediated by P-glycoprotein (P-gp) transmembrane transporters. The present study aimed to investigate nilotinib's affection on the steady-state pharmacokinetics, disposition and cardiotoxicity of DOX. A total of 24 male SpragueDawley rats were randomized into four groups (6 in each) and received the following regimens: saline, intravenous DOX (5 mg/kg) alone, and DOX co-administrated with either 20 or 40 mg/kg nilotinib. Blood was withdrawn at 12 time points till 72 h after DOX injection and the concentrations of DOX and its metabolite doxorubicinol (DOXol) in serum and cardiac tissue were assayed by LCMSMS method. To determine the cardiotoxicity, the following parameters were investigated: creatine kinase, lactate dehydrogenase, malondialdehyde, and superoxide dismutase. Histopathological examination of heart section was carried out to evaluate the extent of cardiotoxicity after treatments. The results showed that pretreatment of 40 mg/kg nilotinib increased the AUC{sub 0t} and C{sub max} of DOX and DOXol. However, their accumulation in cardiac tissue was significantly decreased when compared with the group that received DOX alone. In addition, biochemical and histopathological results showed that 40 mg/kg nilotinib reduced the cardiotoxicity induced by DOX administration. In conclusion, co-administration of nilotinib increased serum exposure, but significantly decreased the accumulation of DOX in cardiac tissue. Consistent with in vitro profile, oral dose of 40 mg/kg nilotinib significantly decreased the cardiotoxicity of DOX in rat by enhancing P-gp activity in the heart.

  7. Transgenic rats overexpressing the human MrgX3 gene show cataracts and an abnormal skin phenotype

    SciTech Connect (OSTI)

    Kaisho, Yoshihiko . E-mail: Kaisho_Yoshihiko@takeda.co.jp; Watanabe, Takuya; Nakata, Mitsugu; Yano, Takashi; Yasuhara, Yoshitaka; Shimakawa, Kozo; Mori, Ikuo; Sakura, Yasufumi; Terao, Yasuko; Matsui, Hideki; Taketomi, Shigehisa

    2005-05-13

    The human MrgX3 gene, belonging to the mrgs/SNSRs (mass related genes/sensory neuron specific receptors) family, was overexpressed in transgenic rats using the actin promoter. Two animal lines showed cataracts with liquification/degeneration and swelling of the lens fiber cells. The transient epidermal desquamation was observed in line with higher gene expression. Histopathology of the transgenic rats showed acanthosis and focal parakeratosis. In the epidermis, there was an increase in cellular keratin 14, keratin 10, and loricrin, as well as PGP 9.5 in innervating nerve fibers. These phenotypes accompanied an increase in the number of proliferating cells. These results suggest that overexpression of the human MrgX3 gene causes a disturbance of the normal cell-differentiation process.

  8. Atrazine-induced reproductive tract alterations after transplacental and/or lactational exposure in male Long-Evans rats

    SciTech Connect (OSTI)

    Rayner, Jennifer L.; Enoch, Rolondo R.; Wolf, Douglas C.; Fenton, Suzanne E. . E-mail: fenton.suzanne@epa.gov

    2007-02-01

    Studies showed that early postnatal exposure to the herbicide atrazine (ATR) delayed preputial separation (PPS) and increased incidence of prostate inflammation in adult Wistar rats. A cross-fostering paradigm was used in this study to determine if gestational exposure to ATR would also result in altered puberty and reproductive tissue effects in the male rat. Timed-pregnant Long-Evans (LE) rats were dosed by gavage on gestational days (GD) 15-19 with 100 mg ATR/kg body weight (BW) or 1% methylcellulose (controls, C). On postnatal day (PND)1, half litters were cross-fostered, creating 4 treatment groups; C-C, ATR-C, C-ATR, and ATR-ATR (transplacental-milk as source, respectively). On PND4, male offspring in the ATR-ATR group weighed significantly less than the C-C males. ATR-ATR male pups had significantly delayed preputial separation (PPS). BWs at PPS for C-ATR and ATR-ATR males were reduced by 6% and 9%, respectively, from that of C-C. On PND120, lateral prostate weights of males in the ATR-ATR group were significantly increased over C-C. Histological examination of lateral and ventral prostates identified an increased distribution of inflammation in the lateral prostates of C-ATR males. By PND220, lateral prostate weights were significantly increased for ATR-C and ATR-ATR, but there were no significant changes in inflammation in either the lateral or ventral prostate. These results suggest that in LE rats, gestational ATR exposure delays PPS when male offspring suckle an ATR dam, but leads to increased lateral prostate weight via transplacental exposure alone. Inflammation present at PND120 does not increase in severity with time.

  9. Inter-strain heterogeneity in rat hepatic transcriptomic responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

    SciTech Connect (OSTI)

    Yao, Cindy Q.; Prokopec, Stephenie D.; Watson, John D.; Pang, Renee; P'ng, Christine; Chong, Lauren C.; Harding, Nicholas J. [Informatics and Biocomputing Platform, Ontario Institute for Cancer Research, Toronto (Canada)] [Informatics and Biocomputing Platform, Ontario Institute for Cancer Research, Toronto (Canada); Pohjanvirta, Raimo [Laboratory of Toxicology, National Institute for Health and Welfare, Kuopio (Finland) [Laboratory of Toxicology, National Institute for Health and Welfare, Kuopio (Finland); Department of Food Hygiene and Environmental Health, University of Helsinki (Finland); Okey, Allan B. [Department of Pharmacology and Toxicology, University of Toronto, Toronto (Canada)] [Department of Pharmacology and Toxicology, University of Toronto, Toronto (Canada); Boutros, Paul C., E-mail: Paul.Boutros@oicr.on.ca [Informatics and Biocomputing Platform, Ontario Institute for Cancer Research, Toronto (Canada); Department of Medical Biophysics, University of Toronto, Toronto (Canada)

    2012-04-15

    The biochemical and toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) have been the subject of intense study for decades. It is now clear that essentially all TCDD-induced toxicities are mediated by DNAprotein interactions involving the Aryl Hydrocarbon Receptor (AHR). Nevertheless, it remains unknown which AHR target genes cause TCDD toxicities. Several groups, including our own, have developed rodent model systems to probe these questions. mRNA expression profiling of these model systems has revealed significant inter-species heterogeneity in rodent hepatic responses to TCDD. It has remained unclear if this variability also exists within a species, amongst rodent strains. To resolve this question, we profiled the hepatic transcriptomic response to TCDD of diverse rat strains (L-E, H/W, F344 and Wistar rats) and two lines derived from L-E H/W crosses, at consistent age, sex, and dosing (100 ?g/kg TCDD for 19 h). Using this uniquely consistent dataset, we show that the majority of TCDD-induced alterations in mRNA abundance are strain/line-specific: only 11 genes were affected by TCDD across all strains, including well-known dioxin-responsive genes such as Cyp1a1 and Nqo1. Our analysis identified two novel universally dioxin-responsive genes as well as 4 genes induced by TCDD in dioxin-sensitive rats only. These 6 genes are strong candidates to explain TCDD-related toxicities, so we validated them using 152 animals in time-course (0 to 384 h) and doseresponse (0 to 3000 ?g/kg) experiments. This study reveals that different rat strains exhibit dramatic transcriptional heterogeneity in their hepatic responses to TCDD and that inter-strain comparisons can help identify candidate toxicity-related genes.

  10. Amifostine, a radioprotectant agent, protects rat brain tissue lipids against ionizing radiation induced damage: An FTIR microspectroscopic imaging study

    SciTech Connect (OSTI)

    Cakmak G.; Miller L.; Zorlu, F.; Severcan, F.

    2012-03-03

    Amifostine is the only approved radioprotective agent by FDA for reducing the damaging effects of radiation on healthy tissues. In this study, the protective effect of amifostine against the damaging effects of ionizing radiation on the white matter (WM) and grey matter (GM) regions of the rat brain were investigated at molecular level. Sprague-Dawley rats, which were administered amifostine or not, were whole-body irradiated at a single dose of 800 cGy, decapitated after 24 h and the brain tissues of these rats were analyzed using Fourier transform infrared microspectroscopy (FTIRM). The results revealed that the total lipid content and CH{sub 2} groups of lipids decreased significantly and the carbonyl esters, olefinic=CH and CH{sub 3} groups of lipids increased significantly in the WM and GM after exposure to ionizing radiation, which could be interpreted as a result of lipid peroxidation. These changes were more prominent in the WM of the brain. The administration of amifostine before ionizing radiation inhibited the radiation-induced lipid peroxidation in the brain. In addition, this study indicated that FTIRM provides a novel approach for monitoring ionizing radiation induced-lipid peroxidation and obtaining different molecular ratio images can be used as biomarkers to detect lipid peroxidation in biological systems.

  11. TCDD dysregulation of 13 AHR-target genes in rat liver

    SciTech Connect (OSTI)

    Watson, John D.; Prokopec, Stephenie D.; Smith, Ashley B.; Okey, Allan B.; Pohjanvirta, Raimo; Boutros, Paul C.

    2014-02-01

    Despite several decades of research, the complete mechanism by which 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other xenobiotic agonists of the aryl hydrocarbon receptor (AHR) cause toxicity remains unclear. While it has been shown that the AHR is required for all major manifestations of toxicity, the specific downstream changes involved in the development of toxic phenotypes remain unknown. Here we examine a panel of 13 genes that are AHR-regulated in many species and tissues. We profiled their hepatic mRNA abundances in two rat strains with very different sensitivities to TCDD: the TCDD-sensitive LongEvans (Turku/AB; LE) and the TCDD-resistant Han/Wistar (Kuopio; H/W). We evaluated doses ranging from 0 to 3000 ?g/kg at 19 h after TCDD exposure and time points ranging from 1.5 to 384 h after exposure to 100 ?g/kg TCDD. Twelve of 13 genes responded to TCDD in at least one strain, and seven of these showed statistically significant inter-strain differences in the time course analysis (Aldh3a1, Cyp1a2, Cyp1b1, Cyp2a1, Fmo1, Nfe2l2 and Nqo1). Cyp2s1 did not respond to TCDD in either rat strain. Five genes exhibited biphasic responses to TCDD insult (Ahrr, Aldh3a1, Cyp1b1, Nfe2l2 and Nqo1), suggesting a secondary event, such as association with additional transcriptional modulators. Of the 12 genes that responded to TCDD during the doseresponse analysis, none had an ED{sub 50} equivalent to that of Cyp1a1, the most sensitive gene in this study, while nine genes responded to doses at least 10100 fold higher, in at least one strain (Ahrr (LE), Aldh3a1 (both), Cyp1a2 (both), Cyp1b1 (both), Cyp2a1 (LE), Inmt (both), Nfe2l2 (LE), Nqo1 (LE) and Tiparp (both)). These data shed new light on the association of the AHR target genes with TCDD toxicity, and in particular the seven genes exhibiting strain-specific differences represent strong candidate mediators of Type-II toxicities. - Highlights: NanoString measured hepatic mRNA molecules following TCDD treatment. TCDD-sensitive LongEvans and TCDD-resistant Han/Wistar rats were compared. Time courses and dose responses were analyzed for AHR-core gene changes. 7 genes displayed inter-strain mRNA differences at times after TCDD exposure. 2 of the AHR-core genes had significant inter-strain differences in their TCDD ED{sub 50}.

  12. Proximal renal tubular injury in rats sub-chronically exposed to low fluoride concentrations

    SciTech Connect (OSTI)

    Crdenas-Gonzlez, Mariana C.; Del Razo, Luz M.; Barrera-Chimal, Jonatan; Jacobo-Estrada, Tania; Lpez-Bayghen, Esther; and others

    2013-11-01

    Fluoride is usually found in groundwater at a very wide range of concentration between 0.5 and 25 ppm. At present, few studies have assessed the renal effects of fluoride at environmentally relevant concentrations. Furthermore, most of these studies have used insensitive and nonspecific biomarkers of kidney injury. The aim of this study was to use early and sensitive biomarkers to evaluate kidney injury after fluoride exposure to environmentally relevant concentrations. Recently weaned male Wistar rats were exposed to low (15 ppm) and high (50 ppm) fluoride concentrations in drinking water for a period of 40 days. At the end of the exposure period, kidney injury biomarkers were measured in urine and renal mRNA expression levels were assessed by real time RT-PCR. Our results showed that the urinary kidney injury molecule (Kim-1), clusterin (Clu), osteopontin (OPN) and heat shock protein 72 excretion rate significantly increased in the group exposed to the high fluoride concentration. Accordingly, fluoride exposure increased renal Kim-1, Clu and OPN mRNA expression levels. Moreover, there was a significant dose-dependent increase in urinary ?-2-microglobulin and cystatin-C excretion rate. Additionally, a tendency towards a dose dependent increase of tubular damage in the histopathological light microscopy findings confirmed the preferential impact of fluoride on the tubular structure. All of these changes occurred at early stages in which, the renal function was not altered. In conclusion using early and sensitive biomarkers of kidney injury, we were able to found proximal tubular alterations in rats sub-chronically exposed to fluoride. - Highlights: Exposure to low concentrations of fluoride induced proximal tubular injury Increase in urinary Kim-1, Clu, OPN and Hsp72 in 50 ppm fluoride-exposed group Increase in urinary B2M and CysC in 15 and 50 ppm fluoride-exposed groups Fluoride exposure increased renal Kim, Clu and OPN mRNA expression levels. Fluoride increased kidney injury biomarkers at stages where eGFR was unaltered.

  13. Ritonavir binds to and downregulates estrogen receptors: Molecular mechanism of promoting early atherosclerosis

    SciTech Connect (OSTI)

    Xiang, Jin; Wang, Ying; Su, Ke; Liu, Min; Hu, Peng-Chao; Ma, Tian; Li, Jia-Xi; Wei, Lei; Zheng, Zhongliang; Yang, Fang

    2014-10-01

    Estrogenic actions are closely related to cardiovascular disease. Ritonavir (RTV), a human immunodeficiency virus (HIV) protease inhibitor, induces atherosclerosis in an estrogen-related manner. However, how RTV induce pathological phenotypes through estrogen pathway remains unclear. In this study, we found that RTV increases thickness of coronary artery walls of Sprague Dawley rats and plasma free fatty acids (FFA) levels. In addition, RTV could induce foam cell formation, downregulate both estrogen receptor ? (ER?) and ER? expression, upregulate G protein-coupled estrogen receptor (GPER) expression, and all of them could be partially blocked by 17?-estradiol (E2), suggesting RTV acts as an antagonist for E2. Computational modeling shows a similar interaction with ER? between RTV and 2-aryl indoles, which are highly subtype-selective ligands for ER?. We also found that RTV directly bound to ER? and selectively inhibited the nuclear localization of ER?, and residue Leu536 in the hydrophobic core of ligand binding domain (LBD) was essential for the interaction with RTV. In addition, RTV did not change the secondary structure of ER?-LBD like E2, which explained how ER? lost the capacity of nuclear translocation under the treatment of RTV. All of the evidences suggest that ritonavir acts as an antagonist for 17?-estradiol in regulating ? subtype estrogen receptor function and early events of atherosclerosis. - Graphical abstract: RTV directly binds to ER? and Leu536 in the hydrophobic core of ligand binding domain is essential for the interaction. - Highlights: RTV increases the thickness of rat coronary artery wall and foam cell formation. RTV downregulates the expression of ER? and ER?. RTV inhibits ER? promoter activity. RTV directly binds to ER? and the key amino acid is Leu536. RTV inhibits the nuclear translocation of ER? and GPER.

  14. Dissolution and clearance of titanium tritide particles in the lungs of F344/Crl rats

    SciTech Connect (OSTI)

    Cheng, Yung-Sung; Snipes, M.B.; Wang, Yansheng

    1995-12-01

    Metal tritides are compounds in which the radioactive isotope tritium, following adsorption onto the metal, forms a stable chemical compound with the metal. When particles of tritiated metals become airborne, they can be inhaled by workers. Because the particles may be retained in the lung for extended periods, the resulting dose will be greater than doses following exposure to tritium gas or tritium oxide (HTO). Particles of triated metals may be dispersed into the air during routine handling, disruption of contaminated metals, or as a result of spontaneous radioactive decay processes. Unlike metal hydrides and deuterides, tritides are radioactive, and the decay of the tritium atoms affects the metal. Because helium is a product of the decay, helium bubbles form within the metal tritide matrix. The pressure from these bubbles leads to respirable particles breaking off from the tritide surface. Our results show that a substantial amount of titanium tritide remains in the rat lung 10 d after intratracheal instillation, confirming results previously obtain in an in vitro dissolution study.

  15. In vivo measurement of total body magnesium and manganese in rats

    SciTech Connect (OSTI)

    Zhang, R.Q.; Ellis, K.J. )

    1989-11-01

    Mg and Mn are essential minerals in many biological processes. Thus knowledge of their absolute amounts and how those amounts may be altered is important. In the past the in vivo measurement of Mg in animals was limited by both the poor geometry of the counting system and the requirement for multiple counts of the animal over several hours. We have developed a neutron activation technique for the direct in vivo measurement of total body Mg and Mn in the rat. The counting system adapted for the technique has a response that is relatively invariant (+/- 2.5%) to differences in body size. A least-squares curve fitting technique was developed that requires only a single 5-min count of the animal. Our in vivo values for body Mg and Mn were in excellent agreement (+/- 2.0%) with the results of total carcass analysis using atomic absorption. Longitudinal changes in total body Mg and Mn were examined in vivo in two groups of animals maintained on test diets that contained different amounts of Mg.

  16. Ethanol-induced cell damage in cultured rat antral mucosa assessed by chromium-51 release

    SciTech Connect (OSTI)

    Sewell, R.B.; Ling, T.S.; Yeomans, N.D.

    1986-08-01

    We have developed an in vitro method for studying ethanol-induced injury to gastric mucosa using organ culture of rat antrum. Cell damage was assessed by measurement of the release of (/sup 51/Cr)sodium chromate from preloaded cells, a method adapted from a standard immunologic technique. This system provided rapid and highly reproducible quantitation of tissue injury as assessed by /sup 51/Cr release into the culture medium. The threshold concentration for ethanol-induced damage was between 10 and 15% v/v, similar to in vivo thresholds observed by others. /sup 51/Cr release could also be induced by very short exposure to ethanol (5-15 min), and then continued despite ethanol removal. Interestingly, after continuous ethanol exposure, a plateau of maximum /sup 51/Cr release was reached 60 min after exposure to ethanol over the concentration range 20-50%, suggesting tissue adaptation to ethanol damage. This organ culture system, which allows precise control of experimental conditions, may be useful for studying mechanisms of gastric mucosal injury and protection.

  17. Effect of. cap alpha. -ketobutyrate on the metabolism of pyruvate and palmitate in isolated rat hepatocytes

    SciTech Connect (OSTI)

    Brass, E.P.

    1986-05-01

    Alpha-ketobutyrate (..cap alpha..KB), an intermediate in the catabolism of threonine and methionine, is decarboxylated to propionyl-CoA. The authors have reported that propionate (PROP) inhibits oxidative metabolism in rate hepatocytes. Based on these observations, the present study examined the effects of ..cap alpha..KB on pyruvate and palmitate metabolism in hepatocytes isolated from fed rats. Similar to PROP, ..cap alpha..KB (10mM) inhibited palmitate oxidation and this inhibition was diminished when 10mM carnitine (CN) was added (35 +/- 6% inhibition without CN, 22 +/- 8% with CN). ..cap alpha..KB inhibited the conversion of 3-/sup 14/C-pyruvate to glucose and CO/sub 2/. Inhibition of pyruvate metabolism by ..cap alpha..KB was concentration-dependent. At equal concentrations, ..cap alpha..KB inhibited pyruvate metabolism to a greater extent than PROP. Addition of CN partially reversed the effects of PROP on pyruvate metabolism, but not those of ..cap alpha..KB despite the generation of propionylcarnitine when ..cap alpha..KB and CN were included in the incubation. These results demonstrate that accumulation of ..cap alpha..KB can impair normal hepatocyte metabolism. While some of the effects of ..cap alpha..KB can be explained on the basis of propionyl-CoA formation, ..cap alpha..KB has effects on pyruvate metabolism not explainable by this mechanism.

  18. Influence of copper and iron on subacute cadmium intoxication in protein-malnourished rats

    SciTech Connect (OSTI)

    Tewari, P.C.; Kachru, D.N.; Tandon, S.K.

    1986-10-01

    Male albino rats maintained on low-protein (9%) diets were dosed intraperitoneally with 0.75 mg Cd/kg, as cadmium chloride, for 20 days. Groups of these animals were provided with diets supplemented with 40 ppm Cu, 400 ppm Fe or a combination of both during the exposure period. Hepatic and renal distribution of Cd, Zn, Cu, and Fe along with activity of acid and alkaline phosphatases and ribonuclease and glutathione content were studied. Uptake of Cd both in liver and in kidney was significant and was accompanied by increased Zn and depletion of Fe concentration. The Cu level remained unaltered. Dietary supplementation of Cu or Fe interacted effectively and influenced the metal distribution. Acid and alkaline phosphatases in both liver and kidney were inhibited by Cd exposure. However, Cu and/or Fe supplements could to a varying degree offset the Cd-induced inhibition. Cadmium exposure did not, however, elicit any effect on hepatic and renal ribonuclease activity of low-protein-fed animals. The glutathione concentration registered profound increase on Cd exposure, possibly to act as a defense mechanism.

  19. Imaging Nicotine in Rat Brain Tissue by Use of Nanospray Desorption Electrospray Ionization Mass Spectrometry

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Thomas, Mathew; Carson, James P.; Smith, Jordan N.; Timchalk, Charles; Laskin, Julia

    2013-01-15

    Imaging mass spectrometry offers simultaneous detection of drugs, drug metabolites and endogenous substances in a single experiment. This is important when evaluating effects of a drug on a complex organ system such as the brain, where there is a need to understand how regional drug distribution impacts function. Nicotine is an addictive drug and its action in the brain is of high interest. Here we use nanospray desorption electrospray ionization, nano-DESI, imaging to discover the localization of nicotine in rat brain tissue after in vivo administration of nicotine. Nano-DESI is a new ambient technique that enables spatially-resolved analysis of tissue samples without special sample pretreatment. We demonstrate high sensitivity of nano-DESI imaging that enables detection of only 0.7 fmole nicotine per pixel in the complex brain matrix. Furthermore, by adding deuterated nicotine to the solvent, we examined how matrix effects, ion suppression, and normalization affect the observed nicotine distribution. Finally, we provide preliminary results suggesting that nicotine localizes to the hippocampal substructure called dentate gyrus.

  20. Uranyl nitrate-exposed rat alveolar macrophages cell death: Influence of superoxide anion and TNF ? mediators

    SciTech Connect (OSTI)

    Orona, N.S.; Tasat, D.R.

    2012-06-15

    Uranium compounds are widely used in the nuclear fuel cycle, military and many other diverse industrial processes. Health risks associated with uranium exposure include nephrotoxicity, cancer, respiratory, and immune disorders. Macrophages present in body tissues are the main cell type involved in the internalization of uranium particles. To better understand the pathological effects associated with depleted uranium (DU) inhalation, we examined the metabolic activity, phagocytosis, genotoxicity and inflammation on DU-exposed rat alveolar macrophages (12.5200 ?M). Stability and dissolution of DU could differ depending on the dissolvent and in turn alter its biological action. We dissolved DU in sodium bicarbonate (NaHCO{sub 3} 100 mM) and in what we consider a more physiological vehicle resembling human internal media: sodium chloride (NaCl 0.9%). We demonstrate that uranyl nitrate in NaCl solubilizes, enters the cell, and elicits its cytotoxic effect similarly to when it is diluted in NaHCO{sub 3}. We show that irrespective of the dissolvent employed, uranyl nitrate impairs cell metabolism, and at low doses induces both phagocytosis and generation of superoxide anion (O{sub 2}{sup ?}). At high doses it provokes the secretion of TNF? and through all the range of doses tested, apoptosis. We herein suggest that at DU low doses O{sub 2}{sup ?} may act as the principal mediator of DNA damage while at higher doses the signaling pathway mediated by O{sub 2}{sup ?} may be blocked, prevailing damage to DNA by the TNF? route. The study of macrophage functions after uranyl nitrate treatment could provide insights into the pathophysiology of uranium?related diseases. -- Highlights: ? Uranyl nitrate effect on cultured macrophages is linked to the doses and independent of its solubility. ? At low doses uranyl nitrate induces generation of superoxide anion. ? At high doses uranyl nitrate provokes secretion of TNF?. ? Uranyl nitrate induces apoptosis through all the range of doses tested.

  1. Anti-inflammatory and anti-oxidant activities of olmesartan medoxomil ameliorate experimental colitis in rats

    SciTech Connect (OSTI)

    Nagib, Marwa M.; Tadros, Mariane G.; ELSayed, Moushira I.; Khalifa, Amani E.

    2013-08-15

    Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) driven through altered immune responses with production of proinflammatory cytokines. Many therapies are used, but side effects and loss of response limit long-term effectiveness. New therapeutic strategies are thus needed for patients who don't respond to current treatments. Recently, there is suggested involvement of the proinflammatory hormone angiotensin II in inflammatory bowel disease. The aim of this study was to investigate the possible role of olmesartan medoxomil (OLM-M), an angiotensin II receptor blocker in ameliorating ulcerative colitis. Colitis was induced in male Wistar rats by administration of 5% dextran sodium sulphate (DSS) in drinking water for 5 days. OLM-M (1, 3 and 10 mg/kg) was administered orally during 21 days prior to the induction of colitis, and for 5 days after. Sulfasalazine (500 mg/kg) was used as reference drug. All animals were tested for changes in colon length, disease activity index (DAI) and microscopic damage. Colon tissue concentration/activity of tumor necrosis alpha (TNF-?), myeloperoxidase (MPO), prostaglandin E2 (PGE2), reduced glutathione (GSH) and malondialdehyde (MDA) were assessed. Results showed that the OLM-M dose-dependently ameliorated the colonic histopathological and biochemical injuries, an effect that is comparable or even better than that of the standard sulfasalazine. These results suggest that olmesartan medoxomil may be effective in the treatment of UC through its anti-inflammatory and antioxidant effects. - Highlights: Olmesartan medoximil reduced dextran sodium sulphate- induced colitis. Mechanism involved anti-inflammatory and antioxidant effects dose- dependently. It suppressed malondialdehyde and restored reduced glutathione levels. It reduced inflammatory markers levels and histological changes.

  2. Interdisciplinary neurotoxicity inhalation studies: Carbon disulfide and carbonyl sulfide research in F344 rats

    SciTech Connect (OSTI)

    Sills, Robert C. [Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, 111 Alexander Drive, South Campus, MD B3-08, PO Box 12233, Research Triangle Park, NC 27709 (United States)]. E-mail: sills@niehs.nih.gov; Harry, G. Jean [Laboratory of Neurobiology, National Institute of Environmental Health Sciences, 111 Alexander Drive, South Campus, PO Box 12233, Research Triangle Park, NC 27709 (United States); Valentine, William M. [Department of Pathology and Center in Molecular Toxicology, Vanderbilt University Medical Center, Nashville, TN 37232 (United States); Morgan, Daniel L. [Laboratory of Molecular Toxicology, NIEHS, Research Triangle Park, NC 27709 (United States)

    2005-09-01

    Inhalation studies were conducted on the hazardous air pollutants, carbon disulfide, which targets the central nervous system (spinal cord) and peripheral nervous system (distal portions of long myelinated axons), and carbonyl sulfide, which targets the central nervous system (brain). The objectives were to investigate the neurotoxicity of these compounds by a comprehensive evaluation of function, structure, and mechanisms of disease. Through interdisciplinary research, the major finding in the carbon disulfide inhalation studies was that carbon disulfide produced intra- and intermolecular protein cross-linking in vivo. The observation of dose-dependent covalent cross-linking in neurofilament proteins prior to the onset of lesions is consistent with this process contributing to the development of the neurofilamentous axonal swellings characteristic of carbon disulfide neurotoxicity. Of significance is that valine-lysine thiourea cross-linking on rat globin and lysine-lysine thiourea cross-linking on erythrocyte spectrin reflect cross-linking events occurring within the axon and could potentially serve as biomarkers of carbon disulfide exposure and effect. In the carbonyl sulfide studies, using magnetic resonance microscopy (MRM), we determined that carbonyl sulfide targets the auditory pathway in the brain. MRM allowed the examination of 200 brain slices and made it possible to identify the most vulnerable sites of neurotoxicity, which would have been missed in our traditional neuropathology evaluations. Electrophysiological studies were focused on the auditory system and demonstrated decreases in auditory brain stem evoked responses. Similarly, mechanistic studies focused on evaluating cytochrome oxidase activity in the posterior colliculus and parietal cortex. A decrease in cytochrome oxidase activity was considered to be a contributing factor to the pathogenesis of carbonyl sulfide neurotoxicity.

  3. Intervention of D-glucose ameliorates the toxicity of streptozotocin in accessory sex organs of rat

    SciTech Connect (OSTI)

    Vikram, A.; Tripathi, D.N.; Ramarao, P. [Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab-160062 (India); Jena, G.B. [Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, Punjab-160062 (India)], E-mail: gbjena@gmail.com

    2008-01-01

    Streptozotocin (STZ) is a naturally occurring compound isolated from Streptomyces achromogens. It is used extensively for inducing diabetes in experimental animals. Diabetes mellitus is known to have proven adverse effects on male sexual organs and their reproductive functions. The atrophy of prostate gland and other organs of the genitourinary tract were observed in experimental diabetic animals. STZ exhibits a structural resemblance to D-glucose due to the presence of sugar moiety in its structure. Pancreatic {beta}-cells mainly contain GLUT1 and GLUT2 glucose transporters. Possibly due to structural resemblance, STZ and D-glucose, share a common recognition site for entry into the {beta}-cells. The objective of the present study is to evaluate the effect of D-glucose on STZ-induced toxicity in accessory sex organs of male rats. Animals were kept on overnight fasting. One group received vehicle and served as negative control, while all other groups were given STZ (45 mg/kg). Animals that received only STZ served as positive control. The effect of D-glucose was studied on STZ treated animals with different dosage of D-glucose (250, 500, 1000 and 2000 mg/kg). Restoration of body weight, plasma glucose and plasma insulin was evident only at 1000 and 2000 mg/kg of D-glucose. The protective effect of D-glucose is evident only when it is administered simultaneously with STZ. In the present investigation, we report that simultaneous administration of D-glucose along with STZ ameliorates STZ-induced toxicity. This is evident from the restoration of accessory sex organ's weight, cellular morphology as well as insulin level.

  4. Maternal exposure to cadmium during gestation perturbs the vascular system of the adult rat offspring

    SciTech Connect (OSTI)

    Ronco, Ana Maria, E-mail: amronco@inta.cl [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile); Montenegro, Marcela; Castillo, Paula; Urrutia, Manuel [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile); Saez, Daniel [Faculty of Veterinary Medicine, University of Chile, Casilla 138-11, Santiago (Chile); Hirsch, Sandra [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile); Zepeda, Ramiro [Faculty of Medicine, University of Chile, Casilla 138-11, Santiago (Chile); Llanos, Miguel N. [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile)

    2011-03-01

    Several cardiovascular diseases (CVD) observed in adulthood have been associated with environmental influences during fetal growth. Here, we show that maternal exposure to cadmium, a ubiquitously distributed heavy metal and main component of cigarette smoke is able to induce cardiovascular morpho-functional changes in the offspring at adult age. Heart morphology and vascular reactivity were evaluated in the adult offspring of rats exposed to 30 ppm of cadmium during pregnancy. Echocardiographic examination shows altered heart morphology characterized by a concentric left ventricular hypertrophy. Also, we observed a reduced endothelium-dependent reactivity in isolated aortic rings of adult offspring, while endothelium-independent reactivity remained unaltered. These effects were associated with an increase of hem-oxygenase 1 (HO-1) expression in the aortas of adult offspring. The expression of HO-1 was higher in females than males, a finding likely related to the sex-dependent expression of the vascular cell adhesion molecule 1 (VCAM-1), which was lower in the adult female. All these long-term consequences were observed along with normal birth weights and absence of detectable levels of cadmium in fetal and adult tissues of the offspring. In placental tissues however, cadmium levels were detected and correlated with increased NF-{kappa}B expression - a transcription factor sensitive to inflammation and oxidative stress - suggesting a placentary mechanism that affect genes related to the development of the cardiovascular system. Our results provide, for the first time, direct experimental evidence supporting that exposure to cadmium during pregnancy reprograms cardiovascular development of the offspring which in turn may conduce to a long term increased risk of CVD.

  5. Electrorheological (ER) fluids: A research needs assessment. Final report

    SciTech Connect (OSTI)

    Krieger, I.M.; Collins, E.A.

    1993-05-01

    This report consists of seven sections: (1) Executive Summary, (2) Introduction, (3) Overview, (4) Recommendations, (5) Panelist Reports, (6) Overseas Research and Development, and (7) Extended Bibliography. The Appendix contains the reports of site visits and contacts and other supplementary documents.

  6. Final Technical Report. FG02-06ER54881

    SciTech Connect (OSTI)

    Lee, Peter; Larbalestier, David

    2014-09-26

    For over 25 years the Applied Superconductivity Center has been integral to the successful development of superconductors for magnetic confinement fusion reactors, culminating in being a part of the dream team assembled by the ITER Organization to address the failure of prototype ITER cable-in-conduit conductors to pass testing in the SULTAN facility, an issue that if not addressed successfully would have limited the useful lifetime of the reactor. While being called on through the years to address the immediate needs of facility construction we also continued fundamental studies of fusion applicable superconductors so that magnet designers could make use of improved performance, reliability and cost. The annual Low Temperature Superconductors workshops, jointly organized with LBNL, became the most important meeting for dissemination of data between university laboratories, national laboratories and industry.

  7. Audit Report: ER-FS-99-01

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    is to inform Chicago Operations Office management concerning matters that came to the attention of the Office of Inspector General during the audit at the Chicago Operations...

  8. Final report on DOE ASCR Grant DESC0001862 (ER25894)

    SciTech Connect (OSTI)

    Tits, Andre L; O'Leary, Dianne P

    2013-08-28

    As was outlined in the proposal, the overall objective of this project is to investigate advanced optimization algorithms for solving entropy maximization problems. Entropy maximization is a general tool for reconstructing the least biased estimate of the state of a complex system, based on available information. In transport and kinetic theory, entropy maximization is used to derive closures for moment models in order to reduce the complexity of a kinetic description while maintaining many of its fundamental features. Application areas include gas dynamics, charged-particle transport, thermal radiative transfer, and neutron transport. Some such applications are of prime importance to the Department of Energy.

  9. CX-00042_ER52_Fire_Station_Warning_Signals.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  10. DOE Award # FC02-07ER25812 Final ...

    Office of Scientific and Technical Information (OSTI)

    ... benefits of a CCA environment, and showed interest in using CCA tools and techniques. However, that work was never performed because of the early termination of the TASCS project. ...

  11. Self-Assembled ErSb Nanostructures with Optical Applications...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: English Subject: solar (photovoltaic), solid state lighting, phonons, thermoelectric, bio-inspired, energy storage (including ...

  12. DOE/ER-0441 Atmospheric Radiation Measurement Plan - February 1990

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1 Atmospheric Radiation Measurement Program Plan ARM Program Plan Forward In 1978 the Department of Energy initiated the Carbon Dioxide Research Program to address climate change from the increasing concentration of carbon dioxide in the atmosphere. Over the years the Program has studied the many facets of the issue, from the carbon cycle, the climate diagnostics, the vegetative effects, to the societal impacts. The Program is presently the Department's principal entry in the U.S. Global Change

  13. DOE Grant DE-FG02-01ER45931

    Office of Scientific and Technical Information (OSTI)

    ... Korea University, Seoul, Korea, April 27, 2010. V.N. Prigodin and A.J. Epstein, Organic Spintronics, Colloquium, Ioffe Institute, St. Petersburg, Russia, May 21, 2010. V.N. ...

  14. 02-10ER85986 | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of oil from water-wet Osorb. The project team demonstrated that other low boiling gases (LPG liquefied petroleum gas and refrigerant R134a) are suitable for use in the...

  15. ER85357_Phase2_Eltron | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    solids from produced water originating from domestic oil and gas production. Treated water can be re-used in the extraction process without coolingre-heating costs or can be...

  16. DOE DE-FG02-07ER64341

    Office of Scientific and Technical Information (OSTI)

    radiotherapy treatment for prostate cancer were analyzed for gene expression using ... Ionizing Radiation Exposure," Clinical Cancer Research, 12, 3723-3729. Lehmann, Joerg, ...

  17. Microsoft Word - ER64694_FInalScientificRpt.docx

    Office of Scientific and Technical Information (OSTI)

    ... C. To validate predicted metabolic potentials by 13C based metabolic flux analysis. II. To develop genetic tools for facile metabolic manipulation of Cyanothece. III.To use ...

  18. Audit Report: ER-B-99-04

    Broader source: Energy.gov [DOE]

    Credit Card Usage at the Ohio Field Office and the Fernald and Miamisburg Environmental Management Projects

  19. final.ER41452.az.burrows.2007.pdf

    Office of Scientific and Technical Information (OSTI)

    i n a l R e p o r t o n t h e A r i z o n a P h a s e o f S c i D A C a w a r d D E - F C 0 2 - 0 6 E R 4 1 4 5 2 a t t h e U n i v e r s i t y o f A r i z o n a A . B u r r o w s 1 1 . I N T R O D U C T I O N T h i s d e c u r a n t s e r \ e s a s t h e f i n a l r e p c c t f o r t h e A r i z o n a p h a s e ( D E - F Q 3 2 - 0 6 E R 4 1 4 5 2 ) o f m y S c iD A C a w a r d ( e n title d " S c iD A C C o m p u t a t i o n a l A s t r o p h y s i c s C o n s o r t i u m " ( C A C )

  20. Audit report: ER-FS-99-02

    Broader source: Energy.gov [DOE]

    Matters Identified At The Oak Ridge Operations Office During the Audit Of The Department's Consolidated Fiscal Year 1998 Financial Statements

  1. Audit Report: ER-FS-99-03

    Broader source: Energy.gov [DOE]

    Matters Identified at the Savannah River Operations Office During the Audit of the Department of Energy's Consolidated Fiscal Year 1998 Financial Statements

  2. ERS 14.2 Emissions Monitoring, 4/3/95

    Broader source: Energy.gov [DOE]

    The objective of this surveillance is to verify that the contractor is monitoring emissions of radioactive materials and chemicals.  The Facility Representative will verify operability of equipment...

  3. Audit Report: ER-FS-99-01

    Broader source: Energy.gov [DOE]

    Results of Audit Procedures Performed at Chicago Operations Office During the Audit of the Department’s Consolidated Fiscal Year 1998 Financial Statements

  4. Audit Report: ER-FS-99-04

    Broader source: Energy.gov [DOE]

    Results of Audit Procedures Performed at the Strategic Petroleum Reserve During the Audit of the Department’s Consolidated Fiscal Year 1998 Financial Statements

  5. HOt Water SavEr (HOWSE) Project. Final report

    SciTech Connect (OSTI)

    Olson, W.R.

    1981-12-31

    The dishwasher effluent is pumped into the flue of the exchange tank by the normal dishwasher pump (or auxiliary pump). The effluent is stored in this tank until next operation of the dishwasher. Thus, thermal equilibrium can be reached between the tank and the effluent, promoting high efficiency. The output from the exchange tank feeds the household normal hot water tank, reducing its requirement for fuel as the input water temperature is higher. Counterflow exchangers may be used for other hot water users where the flow and drain is continuous. In this case the discharged hot (or warm) water flows counter to the flow of cold water into the hot water heater. The two flows are closely coupled thermally but not in direct contract so they cannot mix. Counter flow exchangers and storage type exchangers may be used in the same installation.

  6. Grant No. DE-FG03-86ER-13469

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    6 J Vibrational Spectra of Isomers of C1&: The Role of the Chlorine Oxide Dimer in A - -tic Ozone Depleting Mechanisms", J. Phys. Chem, 94,6126 (1990). McGrath, M. P. and Rowland,...

  7. ER CXD-Form-2011_Ver2a.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Electro-Refining Project (4624 (Rev.1)) Y-12 Site Office Oak Ridge Anderson County Tennessee The purpose of this project is to install and turnover to operations a process...

  8. FINAL REPORT DE-FG02-07ER15894

    SciTech Connect (OSTI)

    Neurock, Matthew

    2013-05-31

    One of the greatest technological hurdles to deployment of fuel cells relates to the sluggish activity, low durability and the high cost of the catalysts that are currently employed. For automotive PEM fuel cells to become commercially viable, the Pt-specific power density would need to be reduced to less than 0.2gPt/kW (at cell voltages >0.65 V). This would require the Pt loadings to be less than 0.15 mgPt/cm2MEA within the membrane electrode assembly. This could be achieved by enhancing the catalytic activity at the cathode, thus lowering its overpotential. Various different Pt-alloys have shown 2-4 times enhanced activities over Pt alone but still suffer some of the same durability issues as that of the pure Pt. There is a general loss of active Pt due to dissolution and sintering. While there have been a number of elegant fundamental experimental and theoretical studies on ideal single crystal Pt and Pt alloy surfaces which have helped to elucidate the factors that control the activity, there have been very few fundamental studies focused on the stability, reactivity and durability of well-defined Pt nanoparticles. We carried out ab initio density functional theory together with a novel double reference method that we developed to simulate constant potential electrochemical systems in order to model the electrocatalytic reduction of oxygen over model Pt alloy surfaces and nanoparticles. These simulations were used to probe the factors that control the electrocatalytic activity, guide the potential selection of new materials and test their stability under reaction conditions. Ab initio calculations were used to determine the reaction energies and activation barriers for a comprehensive array of different elementary adsorption, desorption, surface reaction and diffusion steps over Pt and Pt alloys involved in the electroreduction of oxygen as a function of surface coverage, the electrochemical potential and temperature. The calculations were used to simulate the potential dependent adsorption and surface reaction energies along with activation barriers in order to determine the kinetics for different surface structures and structural features (step edge and corner sites) to provide the necessary input for kinetic Monte Carlo simulations to follow the rates of reaction. More coarse-grained simulated annealing methods were used to help establish the lowest energy structures and morphologies for different Pt and Pt-alloy nanoparticles that form. The results from the DFT calculations were used to establish an ab initio-derived kinetic database that was used in both 2D and 3D kinetic Monte Carlo simulations that were used to follow the electrocatalytic performance over different particle sizes, shapes and compositions. The ab initio calculations together with the kinetic Monte Carlo simulations were used to complete the following objectives: 1) Determine the controlling elementary reaction pathways and intrinsic kinetics involved in ORR and their potential dependent behavior; 2) Establish the influence of the extrinsic reaction environment including surface structure, alloy composition and spatial arrangement and the humidity on ORR kinetics; 3) Elucidate the effects of particle size and morphology as well as the atomic structure and composition of nanoparticles of Pt, Pt3Co, Pt3Ni, Pt3Fe and other Pt-alloys on the electrocatalytic activity and reaction selectivity; 5) Construct surface Pourbaix phase diagrams for different Pt and Pt-alloys in order to map out the most stable surface phases for different material compositions and reaction conditions; 6) Elucidate the mechanisms that control dissolution and re-deposition of Pt.

  9. The new ternary pnictides Er{sub 12}Ni{sub 30}P{sub 21} and Er...

    Office of Scientific and Technical Information (OSTI)

    ... (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved. Country of Publication: United States Language: English Subject: 36 MATERIALS SCIENCE; ...

  10. Final Technical Report: DE-FG03-01ER63099/DE-FG02-01ER63099

    SciTech Connect (OSTI)

    John H. Seinfeld

    2005-02-23

    Organic material contributes {approx}20-50% to the total fine aerosol mass at continental mid-latitudes (Saxena and Hildemann, 1996; Murphy et al., 1998; Peterson and Tyler, 2002; Putaud et al., 2004) and as much as 90% in tropical forested areas (Andreae and Crutzen, 1997; Artaxo et al., 2002). Significant amounts of carbonaceous aerosols are also observed in the free troposphere (Heald et al., 2005). A substantial fraction of the organic component of atmospheric particles consists of water-soluble, possibly multifunctional compounds (Saxena and Hildemann, 1996; Kavouras et al., 1998). It is critical that we understand how organic aerosols and their precursors are transformed in the atmosphere and the dependence of the transformation on the chemical and thermodynamic conditions of the ambient environment: (1) to accurately forecast how changing emissions will impact atmospheric organic aerosol concentrations and properties on the regional to global scale, and (2) to relate atmospheric measurements to sources. A large (but as yet unquantified) fraction of organic aerosol is formed in the atmosphere by precursor gases. In addition, both primary and secondary organic aerosol interact with other gas and aerosol species in the atmosphere so that their properties (i.e., size, hygroscopicity, light absorption and scattering sphere efficiency) can change significantly with time and distance from their source. Organic aerosols (OA) are composed of complex mixtures of different organic species from less-polar organics (n-alkanes, polycyclic aromatic hydrocarbons, fatty alcohols, fatty acids, etc.) to highly polar organics such as dicarboxylic acids and multi-functional organic acids. Studies employing FTIR spectroscopy and NEXAFS have demonstrated the presence of different functional groups such as ketonic and carboxylic groups. Humic-like substances (HULIS) have been identified in aerosols. Field observation and laboratory smog chamber studies have demonstrated that oxidative reactions of biogenic and anthropogenic precursors in the gas phase produce low molecular weight organic acids such as oxalic and other dicarboxylic acids, dicarbonyls and multi-functional organics. Oxidation reactions in the particle phase may also produce oxygenated species, including aldehydes, organic acids, and large molecules such as HULIS. Despite this progress, a significant fraction of atmospheric OA still remains poorly characterized.

  11. Toxicokinetics of ?-thujone following intravenous and gavage administration of ?-thujone or ?- and ?-thujone mixture in male and female F344/N rats and B6C3F1 mice

    SciTech Connect (OSTI)

    Waidyanatha, Suramya; Johnson, Jerry D.; Hong, S. Peter; Robinson, Veronica Godfrey; Gibbs, Seth; Graves, Steven W.; Hooth, Michelle J.; Smith, Cynthia S.

    2013-09-01

    Plants containing thujone have widespread use and hence have significant human exposure. ?-Thujone caused seizures in rodents following gavage administration. We investigated the toxicokinetics of ?-thujone in male and female F344/N rats and B6C3F1 mice following intravenous and gavage administration of ?-thujone or a mixture of ?- and ?-thujone (which will be referred to as ?,?-thujone). Absorption of ?-thujone following gavage administration was rapid without any dose-, species-, sex- or test article-related effect. Absolute bioavailability of ?-thujone following administration of ?-thujone or ?,?-thujone was generally higher in rats than in mice. In rats, females had higher bioavailability than males following administration of either test article although a sex difference was not observed in mice. C{sub max} and AUC{sub ?} increased greater than proportional to the dose in female rats following administration of ?-thujone and in male and female mice following administration of ?,?-thujone suggesting possible saturation of elimination kinetics with increasing dose. Dose-adjusted AUC{sub ?} for male and female rats was 5- to 15-fold and 3- to 24-fold higher than mice counterparts following administration of ?-thujone and ?,?-thujone, respectively (p-value < 0.0001 for all comparisons). Following both intravenous and gavage administration, ?-thujone was distributed to the brains of rats and mice with females, in general, having higher brain:plasma ratios than males. These data are in support of the observed toxicity of ?-thujone and ?,?-thujone where females were more sensitive than males of both species to ?-thujone-induced neurotoxicity. In general there was no difference in toxicokinetics between test articles when normalized to ?-thujone concentration. - Highlights: Absorption of ?-thujone following gavage administration was rapid in rats and mice. Rats undergo higher exposure to ?-thujone than mice. ?-Thujone brain:plasma ratios were greater than 1 in both rats and mice. Brain:plasma ratio in females was higher than in males. These data are in support of the observed neurotoxicity of ?-thujone.

  12. Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium

    SciTech Connect (OSTI)

    Grandfield, Kathryn; Herber, Ralf -Peter; Chen, Ling; Djomehri, Sabra; Tam, Caleb; Lee, Ji -Hyun; Brown, Evan; Woolwine III, Wood R.; Curtis, Don; Ryder, Mark; Schuck, Jim; Webb, Samuel; Landis, William; Ho, Sunita P.

    2015-04-18

    Objective: The objective of this study was to investigate the effect of mechanical strain by mapping physicochemical properties at periodontal ligament (PDL)–bone and PDL–cementum attachment sites and within the tissues per se. Design: Accentuated mechanical strain was induced by applying a unidirectional force of 0.06 N for 14 days on molars in a rat model. The associated changes in functional space between the tooth and bone, mineral forming and resorbing events at the PDL–bone and PDL–cementum attachment sites were identified by using micro-X-ray computed tomography (micro-XCT), atomic force microscopy (AFM), dynamic histomorphometry, Raman microspectroscopy, and AFM-based nanoindentation technique. Results from these analytical techniques were correlated with histochemical strains specific to low and high molecular weight GAGs, including biglycan, and osteoclast distribution through tartrate resistant acid phosphatase (TRAP) staining. Results: Unique chemical and mechanical qualities including heterogeneous bony fingers with hygroscopic Sharpey's fibers contributing to a higher organic (amide III — 1240 cm⁻¹) to inorganic (phosphate — 960 cm⁻¹) ratio, with lower average elastic modulus of 8 GPa versus 12 GPa in unadapted regions were identified. Furthermore, an increased presence of elemental Zn in cement lines and mineralizing fronts of PDL–bone was observed. Adapted regions containing bony fingers exhibited woven bone-like architecture and these regions rich in biglycan (BGN) and bone sialoprotein (BSP) also contained high-molecular weight polysaccharides predominantly at the site of polarized bone growth. Conclusions: From a fundamental science perspective the shift in local properties due to strain amplification at the soft–hard tissue attachment sites is governed by semiautonomous cellular events at the PDL–bone and PDL–cementum sites. Over time, these strain-mediated events can alter the physicochemical properties of tissues per se, and consequently the overall biomechanics of the bone–PDL–tooth complex. From a clinical perspective, the shifts in magnitude and duration of forces on the periodontal ligament can prompt a shift in physiologic mineral apposition in cementum and alveolar bone albeit of an adapted quality owing to the rapid mechanical translation of the tooth.

  13. Strain-guided mineralization in the bone–PDL–cementum complex of a rat periodontium

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Grandfield, Kathryn; Herber, Ralf -Peter; Chen, Ling; Djomehri, Sabra; Tam, Caleb; Lee, Ji -Hyun; Brown, Evan; Woolwine III, Wood R.; Curtis, Don; Ryder, Mark; et al

    2015-04-18

    Objective: The objective of this study was to investigate the effect of mechanical strain by mapping physicochemical properties at periodontal ligament (PDL)–bone and PDL–cementum attachment sites and within the tissues per se. Design: Accentuated mechanical strain was induced by applying a unidirectional force of 0.06 N for 14 days on molars in a rat model. The associated changes in functional space between the tooth and bone, mineral forming and resorbing events at the PDL–bone and PDL–cementum attachment sites were identified by using micro-X-ray computed tomography (micro-XCT), atomic force microscopy (AFM), dynamic histomorphometry, Raman microspectroscopy, and AFM-based nanoindentation technique. Results frommore » these analytical techniques were correlated with histochemical strains specific to low and high molecular weight GAGs, including biglycan, and osteoclast distribution through tartrate resistant acid phosphatase (TRAP) staining. Results: Unique chemical and mechanical qualities including heterogeneous bony fingers with hygroscopic Sharpey's fibers contributing to a higher organic (amide III — 1240 cm⁻¹) to inorganic (phosphate — 960 cm⁻¹) ratio, with lower average elastic modulus of 8 GPa versus 12 GPa in unadapted regions were identified. Furthermore, an increased presence of elemental Zn in cement lines and mineralizing fronts of PDL–bone was observed. Adapted regions containing bony fingers exhibited woven bone-like architecture and these regions rich in biglycan (BGN) and bone sialoprotein (BSP) also contained high-molecular weight polysaccharides predominantly at the site of polarized bone growth. Conclusions: From a fundamental science perspective the shift in local properties due to strain amplification at the soft–hard tissue attachment sites is governed by semiautonomous cellular events at the PDL–bone and PDL–cementum sites. Over time, these strain-mediated events can alter the physicochemical properties of tissues per se, and consequently the overall biomechanics of the bone–PDL–tooth complex. From a clinical perspective, the shifts in magnitude and duration of forces on the periodontal ligament can prompt a shift in physiologic mineral apposition in cementum and alveolar bone albeit of an adapted quality owing to the rapid mechanical translation of the tooth.« less

  14. Combined contributions of over-secreted glucagon-like peptide 1 and suppressed insulin secretion to hyperglycemia induced by gatifloxacin in rats

    SciTech Connect (OSTI)

    Yu, Yunli; Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009 ; Wang, Xinting; Liu, Can; Yao, Dan; Shanghai Institute of Materia Medica, Shanghai 201203 ; Hu, Mengyue; Li, Jia; Hu, Nan; Liu, Li; Liu, Xiaodong

    2013-02-01

    Accumulating evidences have showed that gatifloxacin causes dysglycemia in both diabetic and non-diabetic patients. Our preliminary study demonstrated that gatifloxacin stimulated glucagon-like peptide 1 (GLP-1) secretion from intestinal cells. The aim of the study was to investigate the association between gatifloxacin-stimulated GLP-1 release and dysglycemia in both normal and streptozotocin-induced diabetic rats and explore the possible mechanisms. Oral administration of gatifloxacin (100 mg/kg/day and 200 mg/kg/day) for 3 and 12 days led to marked elevation of GLP-1 levels, accompanied by significant decrease in insulin levels and increase in plasma glucose. Similar results were found in normal rats treated with 3-day gatifloxacin. Gatifloxacin-stimulated GLP-1 release was further confirmed in NCI-H716 cells, which was abolished by diazoxide, a K{sub ATP} channel opener. QT-PCR analysis showed that gatifloxacin also upregulated expression of proglucagon and prohormone convertase 3 mRNA. To clarify the contradiction on elevated GLP-1 without insulinotropic effect, effects of GLP-1 and gatifloxacin on insulin release were investigated using INS-1 cells. We found that short exposure (2 h) to GLP-1 stimulated insulin secretion and biosynthesis, whereas long exposure (24 h and 48 h) to high level of GLP-1 inhibited insulin secretion and biosynthesis. Moreover, we also confirmed gatifloxacin acutely stimulated insulin secretion while chronically inhibited insulin biosynthesis. All the results gave an inference that gatifloxacin stimulated over-secretion of GLP-1, in turn, high levels of GLP-1 and gatifloxacin synergistically impaired insulin release, worsening hyperglycemia. -- Highlights: ? Gatifloxacin induced hyperglycemia both in diabetic rats and normal rats. ? Gatifloxacin enhanced GLP-1 secretion but inhibited insulin secretion in rats. ? Long-term exposure to high GLP-1 inhibited insulin secretion and biosynthesis. ? GLP-1 over-secretion may be involved in gatifloxacin-induced hyperglycemia.

  15. Urine acidification has no effect on peroxisome proliferator-activated receptor (PPAR) signaling or epidermal growth factor (EGF) expression in rat urinary bladder urothelium

    SciTech Connect (OSTI)

    Achanzar, William E. Moyer, Carolyn F.; Marthaler, Laura T.; Gullo, Russell; Chen, Shen-Jue; French, Michele H.; Watson, Linda M.; Rhodes, James W.; Kozlosky, John C.; White, Melvin R.; Foster, William R.; Burgun, James J.; Car, Bruce D.; Cosma, Gregory N.; Dominick, Mark A.

    2007-09-15

    We previously reported prevention of urolithiasis and associated rat urinary bladder tumors by urine acidification (via diet acidification) in male rats treated with the dual peroxisome proliferator-activated receptor (PPAR){alpha}/{gamma} agonist muraglitazar. Because urine acidification could potentially alter PPAR signaling and/or cellular proliferation in urothelium, we evaluated urothelial cell PPAR{alpha}, PPAR{delta}, PPAR{gamma}, and epidermal growth factor receptor (EGFR) expression, PPAR signaling, and urothelial cell proliferation in rats fed either a normal or an acidified diet for 5, 18, or 33 days. A subset of rats in the 18-day study also received 63 mg/kg of the PPAR{gamma} agonist pioglitazone daily for the final 3 days to directly assess the effects of diet acidification on responsiveness to PPAR{gamma} agonism. Urothelial cell PPAR{alpha} and {gamma} expression and signaling were evaluated in the 18- and 33-day studies by immunohistochemical assessment of PPAR protein (33-day study only) and quantitative real-time polymerase chain reaction (qRT-PCR) measurement of PPAR-regulated gene expression. In the 5-day study, EGFR expression and phosphorylation status were evaluated by immunohistochemical staining and egfr and akt2 mRNA levels were assessed by qRT-PCR. Diet acidification did not alter PPAR{alpha}, {delta}, or {gamma} mRNA or protein expression, PPAR{alpha}- or {gamma}-regulated gene expression, total or phosphorylated EGFR protein, egfr or akt2 gene expression, or proliferation in urothelium. Moreover, diet acidification had no effect on pioglitazone-induced changes in urothelial PPAR{gamma}-regulated gene expression. These results support the contention that urine acidification does not prevent PPAR{gamma} agonist-induced bladder tumors by altering PPAR{alpha}, {gamma}, or EGFR expression or PPAR signaling in rat bladder urothelium.

  16. Exposure to As, Cd and Pb-mixture impairs myelin and axon development in rat brain, optic nerve and retina

    SciTech Connect (OSTI)

    Rai, Nagendra Kumar; Ashok, Anushruti; Rai, Asit; Tripathi, Sachin; Nagar, Geet Kumar; Mitra, Kalyan; Bandyopadhyay, Sanghamitra

    2013-12-01

    Arsenic (As), lead (Pb) and cadmium (Cd) are the major metal contaminants of ground water in India. We have reported the toxic effect of their mixture (metal mixture, MM), at human relevant doses, on developing rat astrocytes. Astrocyte damage has been shown to be associated with myelin disintegration in CNS. We, therefore, hypothesized that the MM would perturb myelinating white matter in cerebral cortex, optic nerve (O.N.) and retina. We observed modulation in the levels of myelin and axon proteins, such as myelin basic protein (MBP), proteolipid protein, 2?-, 3?-cyclic-nucleotide-3?-phosphodiesterase, myelin-associated glycoprotein and neurofilament (NF) in the brain of developing rats. Dose and time-dependent synergistic toxic effect was noted. The MBP- and NF-immunolabeling, as well as luxol-fast blue (LFB) staining demonstrated a reduction in the area of intact myelin-fiber, and an increase in vacuolated axons, especially in the corpus-callosum. Transmission electron microscopy (TEM) of O.N. revealed a reduction in myelin thickness and axon-density. The immunolabeling with MBP, NF, and LFB staining in O.N. supported the TEM data. The hematoxylin and eosin staining of retina displayed a decrease in the thickness of nerve-fiber, plexiform-layer, and retinal ganglion cell (RGC) count. Investigating the mechanism revealed a loss in glutamine synthetase activity in the cerebral cortex and O.N., and a fall in the brain derived neurotrophic factor in retina. An enhanced apoptosis in MBP, NF and Brn3b-containing cells justified the diminution in myelinating axons in CNS. Our findings for the first time indicate white matter damage by MM, which may have significance in neurodevelopmental-pediatrics, neurotoxicology and retinal-cell biology. - Highlights: As, Cd and Pb-mixture, at human relevant dose, demyelinate developing rat CNS. The attenuation in myelin and axon is synergistic. The optic nerve and brain demonstrate reduced glutamine synthetase. The retina exhibits diminished neurotrophin levels and cellular differentiation. The toxic effect is apoptotic.

  17. Early life ethanol exposure causes long-lasting disturbances in rat mesenchymal stem cells via epigenetic modifications

    SciTech Connect (OSTI)

    Leu, Yu-Wei; Chu, Pei-Yi; Chen, Chien-Min; Yeh, Kun-Tu; Liu, Yu Ming; Lee, Yen-Hui; Kuo, Shan-Tsu; Hsiao, Shu-Huei

    2014-10-24

    Highlights: Ethanol exposure alters proliferation and differentiation of MSCs. Ethanol exposure suppresses osteogenesis and adipogenesis of MSCs. H3K27me3-associated genes/pathways are affected in ethanol-exposed MSCs. Expression of lineage-specific genes is dysregulated in ethanol-exposed MSCs. - Abstract: Fetal alcohol syndrome (FAS) is a birth defect due to maternal alcohol consumption during pregnancy. Because mesenchymal stem cells (MSCs) are the main somatic stem cells in adults and may contribute to tissue homeostasis and repair in adulthood, we investigated whether early life ethanol exposure affects MSCs and contributes to the propensity for disease onset in later life. Using a rodent model of FAS, we found that ethanol exposure (5.25 g/kg/day) from postnatal days 4 to 9 in rat pups (mimic of human third trimester) caused long-term anomalies in bone marrow-derived MSCs. MSCs isolated from ethanol-exposed animals were prone to neural induction but resistant to osteogenic and adipogenic inductions compared to their age-matched controls. The altered differentiation may contribute to the severe trabecular bone loss seen in ethanol-exposed animals at 3 months of age as well as overt growth retardation. Expression of alkaline phosphatase, osteocalcin, aP2, and PPAR? were substantially inhibited, but BDNF was up-regulated in MSCs isolated from ethanol-exposed 3 month-old animals. Several signaling pathways were distorted in ethanol-exposed MSCs via altered trimethylation at histone 3 lysine 27. These results demonstrate that early life ethanol exposure can have long-term impacts in rat MSCs by both genetic and epigenetic mechanisms.

  18. Prenatal ethanol exposure programs an increased susceptibility of non-alcoholic fatty liver disease in female adult offspring rats

    SciTech Connect (OSTI)

    Shen, Lang; Liu, Zhongfen; Gong, Jun; Zhang, Li; Wang, Linlong; Magdalou, Jacques; Chen, Liaobin; Wang, Hui

    2014-01-15

    Prenatal ethanol exposure (PEE) induces dyslipidemia and hyperglycemia in fetus and adult offspring. However, whether PEE increases the susceptibility to non-alcoholic fatty liver disease (NAFLD) in offspring and its underlying mechanism remain unknown. This study aimed to demonstrate an increased susceptibility to high-fat diet (HFD)-induced NAFLD and its intrauterine programming mechanisms in female rat offspring with PEE. Rat model of intrauterine growth retardation (IUGR) was established by PEE, the female fetus and adult offspring that fed normal diet (ND) or HFD were sacrificed. The results showed that, in PEE + ND group, serum corticosterone (CORT) slightly decreased and insulin-like growth factor-1 (IGF-1) and glucose increased with partial catch-up growth; In PEE + HFD group, serum CORT decreased, while serum IGF-1, glucose and triglyceride (TG) increased, with notable catch-up growth, higher metabolic status and NAFLD formation. Enhanced liver expression of the IGF-1 pathway, gluconeogenesis, and lipid synthesis as well as reduced expression of lipid output were accompanied in PEE + HFD group. In PEE fetus, serum CORT increased while IGF-1 decreased, with low body weight, hyperglycemia, and hepatocyte ultrastructural changes. Hepatic IGF-1 expression as well as lipid output was down-regulated, while lipid synthesis significantly increased. Based on these findings, we propose a two-programming hypothesis for an increased susceptibility to HFD-induced NAFLD in female offspring of PEE. That is, the intrauterine programming of liver glucose and lipid metabolic function is the first programming, and postnatal adaptive catch-up growth triggered by intrauterine programming of GC-IGF1 axis acts as the second programming. - Highlights: Prenatal ethanol exposure increase the susceptibility of NAFLD in female offspring. Prenatal ethanol exposure reprograms fetal livers glucose and lipid metabolism . Prenatal ethanol exposure cause the adaptive change of glucocorticoid-IGF1 axis.

  19. Differential regulation of apoptosis in slow and fast twitch muscles of aged female F344BN rats

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Rice, Kevin M.; Manne, Nandini D. P. K.; Gadde, Murali K.; Paturi, Satyanarayana; Arvapalli, Ravikumar; Blough, Eric

    2015-03-28

    Age-related muscle atrophy is characterized by decreases in muscle mass and is thought be mediated, at least in part, by increases in myocyte apoptosis. Recent data has demonstrated that the degree of muscle loss with aging may differ between males and females while other work has suggested that apoptosis as indicated by DNA fragmentation may be regulated differently in fast- and slow-twitch muscles. Herein, we investigate how aging affects the regulation of muscle apoptosis in the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus muscles of young (6-month), aged (26-month), and very aged (30-month) female Fischer 344/NNiaHSD × Brown Norway/BiNiamore » (F344BN) rats. Tissue sections were stained with hydroethidium for ROS and protein extract was subjected to immunoblotting for assessing apoptotic markers. Our data suggest that decreases in muscle mass were associated with increased DNA fragmentation (TUNEL positive) and increases in reactive oxygen species (ROS) as determined by hydroethidium staining in both the EDL and soleus. Similar to our previous work using aged male animals, we observed that the time course and magnitude of changes in Bax, Bcl-2, caspase-3, caspase-9, and cleavage of α-fodrin protein were regulated differently between muscles. As a result, These data suggest that aging in the female F344BN rat is associated with decreases in muscle mass, elevations in ROS level, increased muscle cell DNA fragmentation, and alterations in cell membrane integrity and that apoptotic mechanisms may differ between fiber types.« less

  20. Site-directed mutagenesis and molecular modelling studies show the role of Asp82 and cysteines in rat acylase 1, a member of the M20 family

    SciTech Connect (OSTI)

    Herga, Sameh; Brutus, Alexandre; Vitale, Rosa Maria; Miche, Helene; Perrier, Josette; Puigserver, Antoine; Scaloni, Andrea; Giardina, Thierry . E-mail: thierry.giardina@univ.u-3mrs.fr

    2005-05-06

    Acylase 1 from rat kidney catalyzes the hydrolysis of acyl-amino acids. Sequence alignment has shown that this enzyme belongs to the metalloprotein family M20. Site-directed mutagenesis experiments led to the identification of one functionally important amino acid residue located near one of the zinc coordinating residues, which play a critical role in the enzymatic activity. The D82N- and D82E-substituted forms showed no significant activity and very low activity, respectively, along with a loss of zinc coordination. Molecular modelling investigations indicated a putative role of D82 in ensuring a proper protonation of catalytic histidine. In addition, none of the five cysteine residues present in the rat kidney acylase 1 sequence seemed involved in the catalytic process: the loss of activity induced by the C294A substitution was probably due to a conformational change in the 3D structure.

  1. Effect of acute treatment with cadmium on ethanol anesthesia, body termperature, and synaptosomal Na/sup +/-K/sup +/-ATPase of rat brain

    SciTech Connect (OSTI)

    Magour, S.; Kristof, V.; Baumann, M.; Assmann, G.

    1981-12-01

    The effect of a single intraperitoneal dose of 0.56, 1.12, and 1.68 mg cadmium/kg on the duration of ethanol-induced sleep was investigated in male rats. Cadmium potentiated ethanol sleeping time in a dose dependent manner up to 300% over controls. No significant difference in the elimination rate of ethanol from blood and brain and observed between control and cadmium-pretreated rats. Cadmium slightly inhibited the hepatic alcohol dehydrogenase in vivo and also potentiated ethanol hypothermia but these changes did not play a significant role in the observed prolongation of ethanol sleeping time. However, cadmium and ethanol additively inhibited brain synaptosomal Na/sup +/-K/sup +/-ATPase in a noncompetitive manner. The results so far indicate that cadmium may increase brain responsiveness toward ethanol partly through inhibition of snaptosomal Na/sup +/-K/sup +/-ATPase.

  2. Changes in expression of renal Oat1, Oat3 and Mrp2 in cisplatin-induced acute renal failure after treatment of JBP485 in rats

    SciTech Connect (OSTI)

    Liu, Tao, E-mail: liutaomedical@qq.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China)] [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Meng, Qiang, E-mail: mengq531@yahoo.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Wang, Changyuan, E-mail: wangcyuan@163.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Liu, Qi, E-mail: llaqii@yahoo.com.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Guo, Xinjin, E-mail: guo.xinjin@163.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China)] [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Sun, Huijun, E-mail: sunhuijun@hotmail.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); Peng, Jinyong, E-mail: jinyongpeng2005@163.com [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China) [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, Dalian 116044 (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Liaoning, Dalian Medical University (China); and others

    2012-11-01

    The purpose of this study is to investigate whether the effect of cyclo-trans-4-L-hydroxyprolyl-L-serine (JBP485) on acute renal failure (ARF) induced by cisplatin is related to change in expression of renal Oat1, Oat3 and Mrp2 in rats. JBP485 reduced creatinine, blood urea nitrogen (BUN) and indoxyl sulfate (IS) in plasma and malondialdehyde (MDA) in kidney, and recovered the glomerular filtration rate (GFR) and the activity of superoxide dismutase (SOD) in cisplatin-treated rats. The plasma concentration of PAH (para-aminohippurate) determined by LCMS/MS was increased markedly after intravenous administration of cisplatin, whereas cumulative urinary excretion of PAH and the uptake of PAH in kidney slices were significantly decreased. qRT-PCR and Western-blot showed a decrease in mRNA and protein of Oat1 and Oat3, an increase in mRNA and protein of Mrp2 in cisplatin-treated rats, and an increase in IS (a uremic toxin) after co-treatment with JBP485. It indicated that JBP485 promoted urinary excretion of toxins by upregulating renal Mrp2. This therefore gives in part the explanation about the mechanism by which JBP485 improves ARF induced by cisplatin in rats. -- Highlights: ? Cisplatin induces acute renal failure (ARF). ? The expression of Oat1, Oat3 and Mrp2 were changed during ARF. ? The regulated expression of Oat1, Oat3 and Mrp2 is an adaptive protected response. ? JBP485 could facilitate the adaptive protective action.

  3. JBP485 improves gentamicin-induced acute renal failure by regulating the expression and function of Oat1 and Oat3 in rats

    SciTech Connect (OSTI)

    Guo, Xinjin [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Meng, Qiang; Liu, Qi; Wang, Changyuan; Sun, Huijun; Peng, Jinyong; Ma, Xiaochi [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Dalian Medical University, Liaoning (China); Kaku, Taiichi [Japan Bioproducts Industry Co. Ltd., Tomigaya, Shibuya-ku, Tokyo (Japan); Liu, Kexin, E-mail: kexinliu@dlmedu.edu.cn [Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian (China); Provincial Key Laboratory for Pharmacokinetics and Transport, Dalian Medical University, Liaoning (China)

    2013-09-01

    We investigated the effects of JBP485 (an anti-inflammatory dipeptide and a substrate of OAT) on regulation of the expression and function of renal Oat1 and Oat3, which can accelerate the excretion of accumulated uremic toxins (e.g. indoxyl sulfate) in the kidney to improve gentamicin-induced ARF in rats. JBP485 caused a significant decrease in the accumulation of endogenous substances (creatinine, blood urea nitrogen and indoxyl sulfate) in vivo, an increase in the excretion of exogenous compounds (lisinopril and inulin) into urine, and up-regulation of the expressions of renal Oat1 and Oat3 in the kidney tissues and slices via substrate induction. To determine the effect of JBP485 on the accelerated excretion of uremic toxins mediated by Oat1 and Oat3, the mRNA and protein expression levels of renal basolateral Oats were assessed by quantitative real-time PCR, western blot, immunohistochemical analysis and an immunofluorescence method. Gentamicin down-regulated the expression of Oats mRNA and protein in rat kidney, and these effects were reversed after administration of JBP485. In addition, JBP485 caused a significant decrease in MPO and MDA levels in the kidney, and improved the pathological condition of rat kidney. These results indicated that JBP485 improved acute renal failure by increasing the expression and function of Oat1 and Oat3, and by decreasing overoxidation of the kidney in gentamicin-induced ARF rats. - Highlights: JBP485 could up-regulate function and expression of Oat1 and Oat3 in kidney. Effects of JBP485 on ARF are mediated by stimulating excretion of uremic toxins. JBP485 protected against gentamicin-induced ARF by decreasing MPO and MDA.

  4. Differential cardiotoxicity in response to chronic doxorubicin treatment in male spontaneous hypertension-heart failure (SHHF), spontaneously hypertensive (SHR), and Wistar Kyoto (WKY) rats

    SciTech Connect (OSTI)

    Sharkey, Leslie C.; Radin, M. Judith; Heller, Lois; Rogers, Lynette K.; Tobias, Anthony; Matise, Ilze; Wang, Qi; Apple, Fred S.; McCune, Sylvia A.

    2013-11-15

    Life threatening complications from chemotherapy occur frequently in cancer survivors, however little is known about genetic risk factors. We treated male normotensive rats (WKY) and strains with hypertension (SHR) and hypertension with cardiomyopathy (SHHF) with 8 weekly doses of doxorubicin (DOX) followed by 12 weeks of observation to test the hypothesis that genetic cardiovascular disease would worsen delayed cardiotoxicity. Compared with WKY, SHR demonstrated weight loss, decreased systolic blood pressure, increased kidney weights, greater cardiac and renal histopathologic lesions and greater mortality. SHHF showed growth restriction, increased kidney weights and renal histopathology but no effect on systolic blood pressure or mortality. SHHF had less severe cardiac lesions than SHR. We evaluated cardiac soluble epoxide hydrolase (sEH) content and arachidonic acid metabolites after acute DOX exposure as potential mediators of genetic risk. Before DOX, SHHF and SHR had significantly greater cardiac sEH and decreased epoxyeicosatrienoic acid (EET) (4 of 4 isomers in SHHF and 2 of 4 isomers in SHR) than WKY. After DOX, sEH was unchanged in all strains, but SHHF and SHR rats increased EETs to a level similar to WKY. Leukotriene D4 increased after treatment in SHR. Genetic predisposition to heart failure superimposed on genetic hypertension failed to generate greater toxicity compared with hypertension alone. The relative resistance of DOX-treated SHHF males to the cardiotoxic effects of DOX in the delayed phase despite progression of genetic disease was unexpected and a key finding. Strain differences in arachidonic acid metabolism may contribute to variation in response to DOX toxicity. - Highlights: Late doxorubicin toxicity evaluated in normal, hypertensive, and cardiomyopathic rats. Hypertension enhances the delayed toxicity of doxorubicin. Genetic predisposition to cardiomyopathy did not further enhance toxicity. Epoxyeicosatrienoic acids increased in response to doxorubicin in SHR and SHHF. Altered leukotriene metabolism may contribute greater toxicity in SHR vs. SHHF rats.

  5. Sprague-Dawley rats display metabolism-mediated sex differences in the acute toxicity of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy)

    SciTech Connect (OSTI)

    Fonsart, Julien [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France); Menet, Marie-Claude [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[Universite Paris Descartes, Faculte de Pharmacie, Plateau Spectrometrie de Masse (IFR 71), Service de Chimie Analytique, Paris F-75006 (France); Decleves, Xavier [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France); Galons, Herve [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[INSERM, U648, Paris F-75006 (France); Crete, Dominique; Debray, Marcel; Scherrmann, Jean-Michel [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France); Noble, Florence [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France)]|[CNRS, UMR 7157, Paris F-75006 (France)]|[INSERM, U705, Paris F-75006 (France)], E-mail: florence.noble@univ-paris5.fr

    2008-07-01

    The use of the amphetamine derivative 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) has been associated with unexplained deaths. Male humans and rodents are more sensitive to acute toxicity than are females, including a potentially lethal hyperthermia. MDMA is highly metabolized to five main metabolites, by the enzymes CYP1A2 and CYP2D. The major metabolite in rats, 3,4-methylenedioxyamphetamine (MDA), also causes hyperthermia. We postulated that the reported sex difference in rats is due to a sexual dimorphism(s). We therefore determined (1) the LD50 of MDMA and MDA, (2) their hyperthermic effects, (3) the activities of liver CYP1A2 and CYP2D, (4) the liver microsomal metabolism of MDMA and MDA, (5) and the plasma concentrations of MDMA and its metabolites 3 h after giving male and female Sprague-Dawley (SD) rats MDMA (5 mg.kg{sup -1} sc). The LD50 of MDMA was 2.4-times lower in males than in females. MDMA induced greater hyperthermia (0.9 deg. C) in males. The plasma MDA concentration was 1.3-fold higher in males, as were CYP1A2 activity (twice) and N-demethylation to MDA (3.3-fold), but the plasma MDMA concentration (1.4-fold) and CYP2D activity (1.3-fold) were higher in females. These results suggest that male SD rats are more sensitive to MDMA acute toxicity than are females, probably because their CYP1A2 is more active, leading to higher N-demethylation and plasma MDA concentration. This metabolic pathway could be responsible for the lethality of MDMA, as the LD50 of MDA is the same in both sexes. These data strongly suggest that the toxicity of amphetamine-related drugs largely depends on metabolic differences.

  6. Toxicity studies on Agents GB and GD (Phase 2): 90-day subchronic study of GB (Sarin, Type I) in CD rats. Final report, Jul 85-Aug 91

    SciTech Connect (OSTI)

    Bucci, T.J.; Parker, R.M.; Crowell, J.A.; Thurman, J.D.; Gosnell, P.A.

    1991-08-01

    A two-phase Dose Range finding study and a 90-Day Subchronic study were conducted in CD rats using the organophosphate ester Sarin (Agent GB, Type I, CAS Number 107-44-8). The highest dose level without lethality in the second phase of the range finding study was designated the maximum tolerated dose (MTD). The doses selected for the subchronic study were the MTD (300 micron GBI/Kg/day), MTD/2 (150, micron GBI/Kg/day), MTD/4 (75 micron GBI/Kg/day), and a vehicle control (O micron /Kg/day). Forty-eight male and forty-eight female CD rats were randomly allocated at 11-12 weeks of age into four treatment groups (12 per sex per group). The animals were gavaged Monday through Friday for 13 weeks and euthanized with carbon dioxide at the beginning of the fourteenth week. Animals were observed daily for clinical signs of toxicity and were weighed weekly. The rats were bled (6 rats/sex/dose) during weeks -1, 1, 3, 7, and at necropsy. Necropsy examination was performed on all animals. Microscopic evaluation was performed on all high-dose and control animals, and on those tissues of lower dose animals that were abnormal at necropsy. All gross lesions and all animals dying or removed early received histological examination. A cause of death or morbidity for animals removed before the end of the study, determined from histopathological examination, was established in four of the eight cases. There were several statistically significant effects in the clinical chemistry and hematology data. These effects were scattered among the treatment groups and were not numerous enough to develop a pattern of organ toxicity.

  7. Effects of prenatal exposure to a low dose atrazine metabolite mixture on pubertal timing and prostate development of male Long-Evans rats

    SciTech Connect (OSTI)

    Stanko, Jason; Enoch, Rolondo; Rayner, Jennifer L; Davis, Christine; Wolf, Douglas; Malarkey, David; Fenton, Suzanne

    2010-12-01

    The present study examines the postnatal reproductive development of male rats following prenatal exposure to an atrazine metabolite mixture (AMM) consisting of the herbicide atrazine and its environmental metabolites diaminochlorotriazine, hydroxyatrazine, deethylatrazine, and deisopropylatrazine. Pregnant Long-Evans rats were treated by gavage with 0.09, 0.87, or 8.73 mg AMM/kg body weight (BW), vehicle, or 100 mg ATR/kg BW positive control, on gestation days 15 19. Preputial separation was significantly delayed in 0.87 mg and 8.73 mg AMM-exposed males. AMM-exposed males demonstrated a significant treatment-related increase in incidence and severity of inflammation in the prostate on postnatal day (PND) 120. A dose-dependent increase in epididymal fat masses and prostate foci were grossly visible in AMM-exposed offspring. These results indicate that a short, late prenatal exposure to mixture of chlorotriazine metabolites can cause chronic prostatitis in male LE rats. The mode of action for these effects is presently unclear.

  8. SU-E-I-34: Intermittent Low- and High-Dose Ethanol Exposure Alters Neurochemical Responses in Adult Rat Brain: An Ex Vivo 1H NMR Spectroscopy at 11.7 T

    SciTech Connect (OSTI)

    Lee, Do-Wan; Kim, Sang-Young; Song, Kyu-Ho; Choe, Bo-Young

    2014-06-01

    Purpose: The first goal of this study was to determine the influence of the dose-dependent effects of intermittent ethanol intoxication on cerebral neurochemical responses among sham controls and low- and high-dose-ethanol-exposed rats with ex vivo high-resolution spectra. The second goal of this study was to determine the correlations between the metabolite-metabolite levels (pairs-of-metabolite levels) from all of the individual data from the frontal cortex of the intermittent ethanol-intoxicated rats. Methods: Eight-week-old male Wistar rats were divided into 3 groups. Twenty rats in the LDE (n = 10) and the HDE (n = 10) groups received ethanol doses of 1.5 g/kg and 2.5 g/kg, respectively, through oral gavage every 8-h for 4 days. At the end of the 4-day intermittent ethanol exposure, one-dimensional ex vivo 500-MHz proton nuclear magnetic resonance spectra were acquired from 30 samples of the frontal cortex region (from the 3 groups). Results: Normalized total-N-acetylaspartate (tNAA: NAA + NAAG [N-acetylaspartyl-glutamate]), gamma-aminobutyric acid (GABA), and glutathione (GSH) levels were significantly lower in the frontal cortex of the HDE-exposed rats than that of the LDE-exposed rats. Moreover, compared to the CNTL group, the LDE rats exhibited significantly higher normalized GABA levels. The 6 pairs of normalized metabolite levels were positively (+) or negatively (?) correlated in the rat frontal cortex as follows: tNAA and GABA (+), tNAA and Aspartate (Asp) (?), myo-Inositol (mIns) and Asp (?), mIns and Alanine (+), mIns and Taurine (+), and mIns and tNAA (?). Conclusion: Our results suggested that repeated intermittent ethanol intoxication might result in neuronal degeneration and dysfunction, changes in the rate of GABA synthesis, and oxidative stress in the rat frontal cortex. Our ex vivo 1H high-resolution-magic angle spinning nuclear magnetic resonance spectroscopy results suggested some novel metabolic markers for the dose-dependent influence of repeated intermittent ethanol intoxication in the frontal cortex.

  9. Characterizing Rat PNS Electrophysiological Response to Electrical Stimulation Using in vitro Chip-Based Human Investigational Platform (iCHIP)

    SciTech Connect (OSTI)

    Khani, Joshua; Prescod, Lindsay; Enright, Heather; Felix, Sarah; Osburn, Joanne; Wheeler, Elizabeth; Kulp, Kris

    2015-08-18

    Ex vivo systems and organ-on-a-chip technology offer an unprecedented approach to modeling the inner workings of the human body. The ultimate goal of LLNL’s in vitro Chip-based Human Investigational Platform (iCHIP) is to integrate multiple organ tissue cultures using microfluidic channels, multi-electrode arrays (MEA), and other biosensors in order to effectively simulate and study the responses and interactions of the major organs to chemical and physical stimulation. In this study, we focused on the peripheral nervous system (PNS) component of the iCHIP system. Specifically we sought to expound on prior research investigating the electrophysiological response of rat dorsal root ganglion cells (rDRGs) to chemical exposures, such as capsaicin. Our aim was to establish a protocol for electrical stimulation using the iCHIP device that would reliably elicit a characteristic response in rDRGs. By varying the parameters for both the stimulation properties – amplitude, phase width, phase shape, and stimulation/ return configuration – and the culture conditions – day in vitro and neural cell types - we were able to make several key observations and uncover a potential convention with a minimal number of devices tested. Future work will seek to establish a standard protocol for human DRGs in the iCHIP which will afford a portable, rapid method for determining the effects of toxins and novel therapeutics on the PNS.

  10. Phase-Contrast MRI and CFD Modeling of Apparent 3He Gas Flow in Rat Pulmonary Airways

    SciTech Connect (OSTI)

    Minard, Kevin R.; Kuprat, Andrew P.; Kabilan, Senthil; Jacob, Rick E.; Einstein, Daniel R.; Carson, James P.; Corley, Richard A.

    2012-08-01

    Phase-contrast (PC) magnetic resonance imaging (MRI) with hyperpolarized 3He is potentially useful for developing and testing patient-specific models of pulmonary airflow. One challenge, however, is that PC-MRI provides apparent values of local 3He velocity that not only depend on actual airflow but also on gas diffusion. This not only blurs laminar flow patterns in narrow airways but also introduces anomalous airflow structure that reflects gas-wall interactions. Here, both effects are predicted in a live rat using computational fluid dynamics (CFD), and for the first time, simulated patterns of apparent 3He gas velocity are compared with in-vivo PC-MRI. Results show (1) that correlations (R2) between measured and simulated airflow patterns increase from 0.23 to 0.79 simply by accounting for apparent 3He transport, and that (2) remaining differences are mainly due to uncertain airway segmentation and partial volume effects stemming from relatively coarse MRI resolution. Higher-fidelity testing of pulmonary airflow predictions should therefore be possible with future imaging improvements.

  11. Metabolomic changes in follicular fluid induced by soy isoflavones administered to rats from weaning until sexual maturity

    SciTech Connect (OSTI)

    Wang, Wenxiang; Zhang, Wenchang; Liu, Jin; Sun, Yan; Li, Yuchen; Li, Hong; Xiao, Shihua; Shen, Xiaohua

    2013-06-15

    Female Wistar rats at 21 days of age were treated with one of three concentrations of soy isoflavones (SIF) (50, 100 or 200 mg/kg body weight, orally, once per day) from weaning until sexual maturity (3 months) in order to evaluate the influence of SIF on ovarian follicle development. After treatment, the serum sex hormone levels and enumeration of ovarian follicles of the ovary were measured. The metabolic profile of follicular fluid was determined using HPLC-MS. Principal component analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA) was used to identify differences in metabolites and reveal useful toxic biomarkers. The results indicated that modest doses of SIF affect ovarian follicle development, as demonstrated by decreased serum estradiol levels and increases in both ovarian follicle atresia and corpora lutea number in the ovary. SIF treatment-related metabolic alterations in follicular fluid were also found in the PCA and PLS-DA models. The 24 most significantly altered metabolites were identified, including primary sex hormones, amino acids, fatty acids and metabolites involved in energy metabolism. These findings may indicate that soy isoflavones affect ovarian follicle development by inducing metabolomic variations in the follicular fluid. - Highlights: ? Modest doses of soy isoflavones (SIF) do affect ovarian follicle development. ? SIF treatment-related metabolic alterations in follicular fluid were found. ? The 24 most significantly altered metabolites were identified.

  12. Toxicity of polychlorinated diphenyl ethers in hydra attenuata and in rat whole-embryo culture. Master's thesis

    SciTech Connect (OSTI)

    Becker, M.C.

    1991-05-01

    Polychlorinated diphenyl ethers (PCDEs) are a class of biaryl compounds that have little commercial application, but appear to be widespread in the environment. They have been found in wood preservative waste dumpsites and in fly ash from municipal waste incinerators. They have been detected in bird eggs and tissues, fish, and other edible marine organisms in the United States, Canada, and Europe. There are limited reports in the extant literature on the toxicity of PCDEs. This study was designed to evaluate the toxicity of selected PCDEs in cultures of Hydra attenuata and post-implantation rat whole embryos. The toxicity of several closely related polychlorinated biphenyls (PCBs) was evaluated in both cultures and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was evaluated in whole embryo culture. Embryonic growth and development parameters (yolk sac diameter, crown-rump length, somite count, and DNA and protein content) and gross morphology were determined. Findings indicated that these chemicals were neither embryotoxic nor teratogenic. Thus, the PCDEs, which elicit other diverse toxic and biochemical responses in rodents, are relatively inactive in these bioassays for developmental toxicity.

  13. Sprague-Dawley rats display sex-linked differences in the pharmacokinetics of 3,4-methylenedioxymethamphetamine (MDMA) and its metabolite 3,4-methylenedioxyamphetamine (MDA)

    SciTech Connect (OSTI)

    Fonsart, Julien, E-mail: julien.fonsart@lrb.aphp.f [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France); CNRS, UMR 7157, Paris F-75006 (France); INSERM, U705, Paris F-75006 (France); Assistance Publique-Hopitaux de Paris, Laboratoire de Toxicologie Biologique, Hopital Lariboisiere, Paris F-75010 (France); Menet, Marie-Claude [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France); Universite Paris Descartes, Faculte de Pharmacie, Plateau Spectrometrie de Masse (IFR 71), Service de Chimie Analytique, Paris F-75006 (France); Debray, Marcel; Hirt, Deborah; Noble, Florence; Scherrmann, Jean-Michel; Decleves, Xavier [Universite Paris Descartes, Faculte de Pharmacie, Paris F-75006 (France); CNRS, UMR 7157, Paris F-75006 (France); INSERM, U705, Paris F-75006 (France)

    2009-12-15

    The use of 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) has increased in recent years; it can lead to life-threatening hyperthermia and serotonin syndrome. Human and rodent males appear to be more sensitive to acute toxicity than are females. MDMA is metabolized to five main metabolites by the enzymes CYP1A2, CYP2D and COMT. Little is presently known about sex-dependent differences in the pharmacokinetics of MDMA and its metabolites. We therefore analyzed MDMA disposition in male and female rats by measuring the plasma and urine concentrations of MDMA and its metabolites using a validated LC-MS method. MDA AUC{sub last} and C{sub max} were 1.6- to 1.7-fold higher in males than in females given MDMA (5 mg/kg sc), while HMMA C{sub max} and AUC{sub last} were 3.2- and 3.5-fold higher, respectively. MDMA renal clearance was 1.26-fold higher in males, and that of MDA was 2.2-fold higher. MDMA AUC{sub last} and t{sub 1/2} were 50% higher in females given MDMA (1 mg/kg iv). MDA C{sub max} and AUC{sub last} were 75-82% higher in males, with a 2.8-fold higher metabolic index. Finally, the AUC{sub last} of MDA was 0.73-fold lower in males given 1 mg/kg iv MDA. The volumes of distribution of MDMA and MDA at steady-state were similar in the two sexes. These data strongly suggest that differences in the N-demethylation of MDMA to MDA are major influences on the MDMA and MDA pharmacokinetics in male and female rats. Hence, males are exposed to significantly more toxic MDA, which could explain previously reported sexual dysmorphism in the acute effects and toxicity of MDMA in rats.

  14. o-p?-DDT-mediated uterotrophy and gene expression in immature C57BL/6 mice and SpragueDawley rats

    SciTech Connect (OSTI)

    Kwekel, Joshua C.; Forgacs, Agnes L.; Williams, Kurt J.; Zacharewski, Timothy R.

    2013-12-15

    1,1,1-Trichloro-2,2-bis(2-chlorophenyl-4-chlorophenyl)ethane (o,p?-DDT) is an organochlorine pesticide and endocrine disruptor known to activate the estrogen receptor. Comprehensive ligand- and species-comparative dose- and time-dependent studies were conducted to systematically assess the uterine physiological, morphological and gene expression responses elicited by o,p?-DDT and ethynyl estradiol (EE) in immature ovariectomized C57BL/6 mice and SpragueDawley rats. Custom cDNA microarrays were used to identify conserved and divergent differential gene expression responses. A total of 1256 genes were differentially expressed by both ligands in both species, 559 of which exhibited similar temporal expression profiles suggesting that o,p?-DDT elicits estrogenic effects at high doses when compared to EE. However, 51 genes exhibited species-specific uterine expression elicited by o,p?-DDT. For example, carbonic anhydrase 2 exhibited species- and ligand-divergent expression as confirmed by quantitative real-time PCR. The identification of comparable temporal phenotypic responses linked to gene expression demonstrates that systematic comparative gene expression assessments are valuable for elucidating conserved and divergent estrogen signaling mechanisms in rodent uterotrophy. - Highlights: o,p?-DDT and enthynyl estradiol (EE) both elicit uterotrophy in mice and rats. o,p?-DDT and EE have different kinetics in uterine wet weight induction. o,p?-DDT elicited stromal hypertrophy in rats but myometrial hypertrophy in mice. 1256 genes were differentially expressed by both ligands in both species. Only 51 genes had species-specific uterine expression.

  15. The potential reproductive, neurobehavioral and systemic effects of soluble sodium tungstate exposure in Sprague-Dawley rats

    SciTech Connect (OSTI)

    McInturf, S.M. [Naval Medical Research Unit at Dayton (NAMRU), Wright Patterson Air Force Base, OH (United States); Bekkedal, M.Y.V. [Two Steps Forward, LLC, Sun Prairie, WI (United States); Wilfong, E. [U.S. Naval Academy, 572M Holloway Road, Annapolis, MD (United States); Arfsten, D. [Navy Drug Screening Laboratory P.O. Box 113, Naval Air Station Jacksonville, FL (United States); Chapman, G. [Naval Medical Research Unit at Dayton (NAMRU), Wright Patterson Air Force Base, OH (United States); Gunasekar, P.G., E-mail: palur.gunasekar@wpafb.af.mil [Naval Medical Research Unit at Dayton (NAMRU), Wright Patterson Air Force Base, OH (United States)

    2011-07-15

    The debate on tungsten (W) is fostered by its continuous usage in military munitions. Reports demonstrate W solubilizes in soil and can migrate into drinking water supplies and, therefore, is a potential health risk to humans. This study evaluated the reproductive, systemic and neurobehavioral effects of sodium tungstate (NaW) in rats following 70 days of daily pre-and postnatal exposure via oral gavage to 5, 62.5 and 125 mg/kg/day of NaW through mating, gestation and weaning (PND 0-20). Daily administration of NaW produced no overt evidence of toxicity and had no apparent effect on mating success or offspring physical development. Distress vocalizations were elevated in F{sub 1} offspring from the high dose group, whereas righting reflex showed unexpected sex differences where males demonstrated faster righting than females; however, the effects were not dose-dependent. Locomotor activity was affected in both low and high-dose groups of F{sub 1} females. Low-dose group showed increased distance traveled, more time in ambulatory movements and less time in stereotypic behavior than controls or high dose animals. The high-dose group had more time in stereotypical movements than controls, and less time resting than controls and the lowest exposure group. Maternal retrieval was not affected by NaW exposure. Tungsten analysis showed a systemic distribution of NaW in both parents and offspring, with preferential uptake within the immune organs, including the femur, spleen and thymus. Histopathological evidence suggested no severe chronic injury or loss of function in these organs. However, the heart showed histological lesions, histiocytic inflammation from minimal to mild with cardiomyocyte degeneration and necrosis in several P{sub 0} animals of 125 mg NaW dose group. The result of this study suggests that pre and postnatal exposure to NaW may produce subtle neurobehavioral effects in offspring related to motor activity and emotionality.

  16. A STUDY OF FISCHER 344 RATS EXPOSED TO SILICA DUST AT CONCENTRATIONS OF 0, 2, 10 OR 20 MG/M3, THEN MAINTAINED FOR SIX MONTHS PRIOR TO ASSESSMENT.

    SciTech Connect (OSTI)

    KUTZMAN,R.S.

    1984-11-01

    The major objective of this study was to relate the results of a series of functional tests to the compositional and structural alterations in the rat lung induced by subchronic exposure to silica dust. To induce a fibrotic lesion, Fischer-344 rats were exposed to either 0, 2, 10, or 20 mg Si0{sub 2}/m{sup 3} for 6 hours/day, 5 days/week for six months and then maintained in an animal room, equipped with a laminar flow unit, for six months prior to assessment of the end points.

  17. Effects of potassium or potassium/magnesium supplementation on potassium content of body tissues and fluids in furosemide-treated rats on magnesium-deficient or magnesium-sufficient diet

    SciTech Connect (OSTI)

    Coram, W.M.; Kapeghian, J.C.; Plocinski, A.F.; Toledo, L.M.; Douglas, F.L.; Weiss, G.B. )

    1990-01-01

    Persistent Mg{sup 2+} deficiency may interfere with restoration of normal tissue K{sup +} levels. This study examined: (a) the effects of chronic furosemide treatment of K{sup +} of sartorius, aorta and ventricle of rats fed Mg{sup 2+}-deficient or Mg{sup 2+} sufficient diet and deionized water; (b) whether normal tissue K{sup +} is restored by oral K{sup +} or K{sup +}/Mg{sup 2+} supplementation with continued furosemide therapy. Levels of Mg{sup 2+} were also measured. Furosemide decreased K{sup +} in sartorius, aorta and ventricle by 5.5, 4.3 and 19.9 {mu}Eq/gm, respectively, in rats fed 100 ppm Mg{sup 2+} diet. Furosemide did not alter K{sup +} levels in rats fed 400 ppm Mg{sup 2+} diet. K{sup +} supplementation restored K{sup +} to normal in sartorius but the addition of Mg{sup 2+} supplementation was necessary to restore K+ levels to normal in ventricle and aorta. These data indicate that furosemide can decrease tissue K{sup +} in rats on a Mg{sup 2+}- deficient diet. This decrease can be reversed during diuretic administration by K{sup +} supplementation in sartorius, or K{sup +} plus Mg{sup 2+} supplementation in ventricle and aorta.

  18. Phosphorus-31 NMR magnetization-transfer measurements of ATP turnover during steady-state isometric muscle contraction in the rat hind limb in vivo

    SciTech Connect (OSTI)

    Brindle, K.M.; Blackledge, M.J.; Challiss, R.A.J.; Radda, G.K. )

    1989-05-30

    Phosphorus-31 NMR magnetization-transfer measurement have been used to measure the flux between ATP and inorganic phosphate during steady-state isometric muscle contraction in the rat hind limb in vivo. Steady-state contraction was obtained by supramaximal sciatic nerve stimulation. Increasing the stimulation pulse width from 10 to 90 ms, at a pulse frequency of 1 Hz, or increasing the frequency of a 10-ms pulse from 0.5 to 2 Hz resulted in an increase in the flux which was an approximately linear function of the increase in the tension-time integral. The flux showed an approximately linear dependence on the calculated free cytosolic ADP concentration up to an ADP concentration of about 90 {mu}M. The data are consistent with control of mitochondrial ATP synthesis by the cytosolic ADP concentration and indicate that the apparent K{sub m} of the mitochondria for ADP is at least 30 {mu}M.

  19. Radiation leukaemogenesis at low doses DE-FG02-05 ER 63947 Final Technical Report 15 May 2005 ???????????????¢???????????????????????????????? 14 May 2010

    SciTech Connect (OSTI)

    Simon Bouffler

    2010-07-28

    This report provides a complete summary of the work undertaken and results obtained under US Department of Energy grant DF-FG02-05 ER 63947, Radiation leukaemogenesis at low doses. There is ample epidemiological evidence indicating that ionizing radiation is carcinogenic in the higher dose range. This evidence, however, weakens and carries increasing uncertainties at doses below 100-200 mSv. At these low dose levels the form of the dose-response curve for radiation-induced cancer cannot be determined reliably or directly from studies of human populations. Therefore animal, cellular and other experimental systems must be employed to provide supporting evidence on which to base judgements of risk at low doses. Currently in radiological protection a linear non-threshold (LNT) extrapolation of risk estimates derived from human epidemiological studies is used to estimate risks in the dose range of interest for protection purposes. Myeloid leukaemias feature prominently among the cancers associated with human exposures to ionising radiation (eg UNSCEAR 2006; IARC 2000). Good animal models of radiation-induced acute myeloid leukaemia (AML) are available including strains such as CBA, RFM and SJL (eg Major and Mole 1978; Ullrich et al 1976; Resnitzky et al 1985). Early mechanistic studies using cytogenetic methods in these mouse models established that the majority of radiation-induced AMLs carried substantial interstitial deletions in one copy of chromosome (chr) 2 (eg Hayata et al 1983; Trakhtenbrot et al 1988; Breckon et al 1991; Rithidech et al 1993; Bouffler et al 1996). Chr2 aberrations are known to occur in bone marrow cells as early as 24 hours after in vivo irradiation (Bouffler et al 1997). Subsequent molecular mapping studies defined a distinct region of chr2 that is commonly lost in AMLs (Clark et al 1996; Silver et al 1999). Further, more detailed, analysis identified point mutations at a specific region of the Sfpi1/PU.1 haemopoietic transcription factor gene which lies in the commonly deleted region of chr2 (Cook et al 2004; Suraweera et al 2005). These lines of evidence strongly implicate the Sfpi1/PU.1 gene as a tumour suppressor gene, dysregulation of which leads to myeloid leukaemia. The main focus of this project was to utilize the CBA mouse model of radiation leukaemogenesis to explore mechanisms of low dose and low dose-rate leukaemogenesis. A series of mechanistic investigations were undertaken, the central aim of which was to identify the events that convert normal cells into myeloid leukaemia cells and explore the dose-response relationships for these. Much of the work centred on the Sfpi1/PU.1 gene and its role in leukaemogenesis. Specific studies considered the dose-response and time-course relationships for loss of the gene, the functional consequences of Sfpi1/PU.1 loss and mutation on transcriptional programmes and developing an in vivo reporter gene system for radiation-induced alterations to PU.1 expression. Additional work sought further genetic changes associated with radiation-induced AMLs and a better characterization of the cell of origin or 'target cell' for radiation-induced AML. All the information gathered is of potential use in developing biologically realistic mathematical models for low dose cancer risk projection.

  20. Acute ethanol intake induces superoxide anion generation and mitogen-activated protein kinase phosphorylation in rat aorta: A role for angiotensin type 1 receptor

    SciTech Connect (OSTI)

    Yogi, Alvaro; Callera, Glaucia E.; Mecawi, Andr S.; Batalho, Marcelo E.; Carnio, Evelin C.; Antunes-Rodrigues, Jos; Queiroz, Regina H.; Touyz, Rhian M.; Tirapelli, Carlos R.

    2012-11-01

    Ethanol intake is associated with increase in blood pressure, through unknown mechanisms. We hypothesized that acute ethanol intake enhances vascular oxidative stress and induces vascular dysfunction through reninangiotensin system (RAS) activation. Ethanol (1 g/kg; p.o. gavage) effects were assessed within 30 min in male Wistar rats. The transient decrease in blood pressure induced by ethanol was not affected by the previous administration of losartan (10 mg/kg; p.o. gavage), a selective AT{sub 1} receptor antagonist. Acute ethanol intake increased plasma renin activity (PRA), angiotensin converting enzyme (ACE) activity, plasma angiotensin I (ANG I) and angiotensin II (ANG II) levels. Ethanol induced systemic and vascular oxidative stress, evidenced by increased plasma thiobarbituric acid-reacting substances (TBARS) levels, NAD(P)H oxidase?mediated vascular generation of superoxide anion and p47phox translocation (cytosol to membrane). These effects were prevented by losartan. Isolated aortas from ethanol-treated rats displayed increased p38MAPK and SAPK/JNK phosphorylation. Losartan inhibited ethanol-induced increase in the phosphorylation of these kinases. Ethanol intake decreased acetylcholine-induced relaxation and increased phenylephrine-induced contraction in endothelium-intact aortas. Ethanol significantly decreased plasma and aortic nitrate levels. These changes in vascular reactivity and in the end product of endogenous nitric oxide metabolism were not affected by losartan. Our study provides novel evidence that acute ethanol intake stimulates RAS activity and induces vascular oxidative stress and redox-signaling activation through AT{sub 1}-dependent mechanisms. These findings highlight the importance of RAS in acute ethanol-induced oxidative damage. -- Highlights: ? Acute ethanol intake stimulates RAS activity and vascular oxidative stress. ? RAS plays a role in acute ethanol-induced oxidative damage via AT{sub 1} receptor activation. ? Translocation of p47phox and MAPKs phosphorylation are downstream effectors. ? Acute ethanol consumption increases the risk for acute vascular injury.

  1. Androgenic endocrine disruptors in wastewater treatment plant effluents in India: Their influence on reproductive processes and systemic toxicity in male rats

    SciTech Connect (OSTI)

    Kumar, Vikas; Chakraborty, Ajanta; Viswanath, Gunda; Roy, Partha

    2008-01-01

    Endocrine-disrupting chemicals (EDC) are linked to human health and diseases as they mimic or block the normal functioning of endogenous hormones. The present work dealt with a comparative study of the androgenic potential of wastewater treatment plant (WWTP) influents and effluents in Northern region of India, well known for its polluted water. Water samples were screened for their androgenic potential using the Hershberger assay and when they were found positive for androgenicity, we studied their mode of action in intact rats. The data showed a significant change in the weight and structure of sex accessory tissues (SATs) of castrated and intact rats. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis demonstrated a significant change in the expression patterns of the major steroidogenic enzymes in adrenal and testis: cytochrome P450{sub SCC}, cytochrome P450{sub C17}, 3{beta}-hydroxysteroid dehydrogenase, 17{beta}-hydroxysteroid dehydrogenase. This was further supported by increased enzymatic activities measured in vitro spectrophotometrically. Serum hormone profile showed a decreased level of gonadotrophic hormones and increased testosterone level. Further, increase in the serum level of alkaline phosphatase, SGPT and SGOT and histopathological changes in kidney and liver of treated animals, confirmed the toxic effects of contaminating chemicals. Analysis of water samples using HPLC and GC-MS showed the presence of various compounds and from them, four prominent aromatic compounds viz. nonylphenol, hexachlorobenzene and two testosterone equivalents, were identified. Our data suggest that despite rigorous treatment, the final treated effluent from WWTP still has enough androgenic and toxic compounds to affect general health.

  2. Oral administration of drugs with hypersensitivity potential induces germinal center hyperplasia in secondary lymphoid organ/tissue in Brown Norway rats, and this histological lesion is a promising candidate as a predictive biomarker for drug hypersensitivity occurrence in humans

    SciTech Connect (OSTI)

    Tamura, Akitoshi Miyawaki, Izuru; Yamada, Toru; Kimura, Juki; Funabashi, Hitoshi

    2013-08-15

    It is important to evaluate the potential of drug hypersensitivity as well as other adverse effects during the preclinical stage of the drug development process, but validated methods are not available yet. In the present study we examined whether it would be possible to develop a new predictive model of drug hypersensitivity using Brown Norway (BN) rats. As representative drugs with hypersensitivity potential in humans, phenytoin (PHT), carbamazepine (CBZ), amoxicillin (AMX), and sulfamethoxazole (SMX) were orally administered to BN rats for 28 days to investigate their effects on these animals by examinations including observation of clinical signs, hematology, determination of serum IgE levels, histology, and flow cytometric analysis. Skin rashes were not observed in any animals treated with these drugs. Increases in the number of circulating inflammatory cells and serum IgE level did not necessarily occur in the animals treated with these drugs. However, histological examination revealed that germinal center hyperplasia was commonly induced in secondary lymphoid organs/tissues in the animals treated with these drugs. In cytometric analysis, changes in proportions of lymphocyte subsets were noted in the spleen of the animals treated with PHT or CBZ during the early period of administration. The results indicated that the potential of drug hypersensitivity was identified in BN rat by performing histological examination of secondary lymphoid organs/tissues. Data obtained herein suggested that drugs with hypersensitivity potential in humans gained immune reactivity in BN rat, and the germinal center hyperplasia induced by administration of these drugs may serve as a predictive biomarker for drug hypersensitivity occurrence. - Highlights: We tested Brown Norway rats as a candidate model for predicting drug hypersensitivity. The allergic drugs did not induce skin rash, whereas D-penicillamine did so in the rats. Some of allergic drugs increased inflammatory cells and IgE, but the others did not. The allergic drugs commonly induced germinal center hyperplasia in lymphoid tissues. Some of these allergic drugs transiently increased CD4{sup +}CD25{sup +} T cells in the spleen.

  3. Sex ratio of the offspring of Sprague-Dawley rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in utero and lactationally in a three-generation study

    SciTech Connect (OSTI)

    Rowlands, J.C. [Dow Chemical Company, Toxicology and Environmental Research and Consulting, 1803 Building, Midland, MI 48674 (United States); Budinsky, R.A. [Dow Chemical Company, Toxicology and Environmental Research and Consulting, 1803 Building, Midland, MI 48674 (United States)]. E-mail: RABudinsky@dow.com; Aylward, L.L. [Summit Toxicology, L.L.P., 6343 Carolyn Drive, Falls Church, VA 22044 (United States); Faqi, A.S. [MPI Research, Department of Developmental and Reproductive Toxicology, 54943 N. Main Street, Mattawan, MI 49071 (United States); Carney, E.W. [Dow Chemical Company, Toxicology and Environmental Research and Consulting, 1803 Building, Midland, MI 48674 (United States)

    2006-10-01

    Reports of a decreased male/female sex ratio in children born to males exposed to TCDD in Seveso, Italy, at a young age have sparked examinations of this endpoint in other populations exposed to TCDD or related compounds. Overall, the male/female sex ratio results reported in these studies, with slightly different age-exposed male populations, have shown mixed results. Experimental studies of the effects of in utero exposure to TCDD in laboratory animals have reported no effect on the f{sub 1} sex ratio and mixed results for the sex ratio of the f{sub 2} generation. In order to better understand the potential effects of TCDD on second generation sex ratio, we retrieved archived data from a comprehensive three-generation feeding study of TCDD in rats that was conducted and published in the 1970s, but which did not publish data on sex ratio of the offspring [Murray, F.J., Smith, F.A., Nitschke, K.D., Humiston, C.G., Kociba, R.J., Schwetz, B.A., 1979. Three-generation reproduction study of rats given 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the diet. Toxicol. Appl. Pharmacol. 50, 241-252]. A re-examination of the original Murray et al. data found no statistically significant treatment-related changes in postnatal day 1 sex ratio in any generation of treated animals, consistent with one other relatively large study reporting on this endpoint. We discuss mechanistic data underlying a potential effect of TCDD on this endpoint. We conclude that the inconsistency in findings on sex ratio of the offspring of male rats exposed to TCDD in utero is likely due to random variation associated with a relatively small sample size, although differences between studies in strain of rat, dose regimen, and day of ascertainment of sex ratio cannot be ruled out.

  4. Exposure to Pb, Cd, and As mixtures potentiates the production of oxidative stress precursors: 30-day, 90-day, and 180-day drinking water studies in rats

    SciTech Connect (OSTI)

    Whittaker, Margaret H.; Wang, Gensheng; Chen Xueqing; Lipsky, Michael; Smith, Donald; Gwiazda, Roberto; Fowler, Bruce A.

    2011-07-15

    Exposure to chemical mixtures is a common and important determinant of toxicity and is of particular concern due to their appearance in sources of drinking water. Despite this, few in vivo mixture studies have been conducted to date to understand the health impact of chemical mixtures compared to single chemicals. Interactive effects of lead (Pb), cadmium (Cd) and arsenic (As) were evaluated in 30-, 90-, and 180-day factorial design drinking water studies in rats designed to test the hypothesis that ingestion of such mixtures at individual component Lowest-Observed-Effect-Levels (LOELs) results in increased levels of the pro-oxidant delta aminolevulinic acid (ALA), iron, and copper. LOEL levels of Pb, Cd, and As mixtures resulted in the increased presence of mediators of oxidative stress such as ALA, copper, and iron. ALA increases were followed by statistically significant increases in kidney copper in the 90- and 180-day studies. Statistical evidence of interaction was identified for six biologically relevant variables: blood delta aminolevulinic acid dehydratase (ALAD), kidney ALAD, urinary ALA, urinary iron, kidney iron, and kidney copper. The current investigations underscore the importance of considering interactive effects that common toxic agents such as Pb, Cd, and As may have upon one another at low-dose levels. The interactions between known toxic trace elements at biologically relevant concentrations shown here demonstrate a clear need to rigorously review methods by which national/international agencies assess health risks of chemicals, since exposures may commonly occur as complex mixtures.

  5. Final Report for DOE Project Number: DE-FG02-05ER46241

    SciTech Connect (OSTI)

    Gang Chen; Mildred S. Dresselhaus; Costas P. Grigoropoulos; Samuel S. Mao; Xiaodong Xiang; Taofang Zeng

    2010-03-15

    Hydrogen storage is the most challenging task for the hydrogen economy. We established a multidisciplinary research program for high throughput combinatorial synthesis and characterization of novel nanoporous and metastable complex hydrides, coupled to fundamental material studies including electronic, structural and kinetic transport modeling, and pump-probe experiments. Our research is based the concept of hybrid nanostructures that store hydrogen by a combination of chemi- and physorption: atomic hydrogen is stored in metastable hydrides while molecule hydrogen is stored in the nanometer pores of the hydrides. Metastable nanostructured hydride has been achieved by introducing structural and compositional disorders through high throughput elemental substitution/doping, catalyst addition, and nonequilibrium processing. Fast screening compatible with the combinatorial synthesis was achieved by combining X-ray structural characterization with the development of a laser-based microbalance. Manufacturing of nanoporous metahydrides that are identified as promising by the combinatorial synthesis has been explored along with the materials search.

  6. Materials Data on Er3InN (SG:221) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. FINAL REPORT Plant Physiological Aspects of Silicon DE-FG03-94ER20151

    Office of Scientific and Technical Information (OSTI)

    and in the interactions between plants and their environment, including gravity, wind, microorganisms, and insects. Having produced the first quantitative evidence of...

  8. Final technical report on Award No. DE-FG02-92ER45465

    SciTech Connect (OSTI)

    Carter, C. Barry

    2003-06-18

    The motivation of the previous program was to develop methods which could be used in a systematic study of the interface between glass and crystalline oxides. It was recognized that since the mechanical properties of glass-containing oxides are particularly important, it would be desirable to have a method which would also allow a systematic comparison of the mechanical properties of the materials. We found that the geometry of a glass film on a crystalline surface gave several new possibilities: we could develop methods for examining the mechanical properties of the films and the interfaces and we could examine both the crystallization of glass films and their wetting/dewetting behavior. Also, the samples are amenable to examination by the characterization techniques which give information on the chemistry and structure over a wide range of length scales: both chemistry and crystallography can be controlled and changes monitored.

  9. Tunable cw laser action of Er sup 3+ in double sensitized fluoroaluminate glass at room temperature

    SciTech Connect (OSTI)

    Heumann, E.; Ledig, M.; Ehrt, D.; Seeber, W. ); Duczynski, E.W.; v.d. Heide, H.J.; Huber, G. )

    1989-10-20

    An efficient energy transfer from chromium via ytterbium to erbium are reported together with the first observation of lasing in fluoroaluminate glass. Lasing can be obtained in only low erbium concentrations. (AIP)

  10. Materials Data on ErGe (SG:63) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. Audit Report: ER-B-00-01 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    a make-or-buy analysis, whether their services could be obtained at a lower cost by outsourcing to commercial entities. The objective of the audit was to determine whether...

  12. Closeout of the award DE-FG02-05ER46223. Trustees of the University...

    Office of Scientific and Technical Information (OSTI)

    Research Org: University of Pennsylvania, Philadelphia (United States). Dept. of Biochemistry & Biophysics, P. Leslie Dutton Sponsoring Org: USDOE Country of Publication: United ...

  13. SERDP ER-1421 Abiotic and Biotic Mechanisms Controlling In Situ Remediation of NDMA: Final Report

    SciTech Connect (OSTI)

    Szecsody, James E.; McKinley, James P.; Crocker, Fiona H.; Breshears, Andrew T.; Devary, Brooks J.; Fredrickson, Herbert L.; Thompson, Karen T.

    2009-09-30

    This laboratory-scale project was initiated to investigate in situ abiotic/biotic mineralization of NDMA. Under iron-reducing conditions, aquifer sediments showed rapid abiotic NDMA degradation to dimethylamine (DMA), nitrate, formate, and finally, CO2. These are the first reported experiments of abiotic NDMA mineralization. The NDMA reactivity of these different iron phases showed that adsorbed ferrous iron was the dominant reactive phase that promoted NDMA reduction, and other ferrous phases present (siderite, iron sulfide, magnetite, structural ferrous iron in 2:1 clays) did not promote NDMA degradation. In contrast, oxic sediments that were biostimulated with propane promoted biomineralization of NDMA by a cometabolic monooxygenase enzyme process. Other monooxygenase enzyme processes were not stimulated with methane or toluene additions, and acetylene addition did not block mineralization. Although NDMA mineralization extent was the highest in oxic, biostimulated sediments (30 to 82%, compared to 10 to 26% for abiotic mineralization in reduced sediments), large 1-D column studies (high sediment/water ratio of aquifers) showed 5.6 times higher NDMA mineralization rates in reduced sediment (half-life 410 147 h) than oxic biomineralization (half life 2293 1866 h). Sequential reduced/oxic biostimulated sediment mineralization (half-life 3180 1094 h) was also inefficient compared to reduced sediment. These promising laboratory-scale results for NDMA mineralization should be investigated at field scale. Future studies of NDMA remediation should focus on the comparison of this in situ abiotic NDMA mineralization (iron-reducing environments) to ex situ biomineralization, which has been shown successful in other studies.

  14. Materials Data on ErInAu (SG:189) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  15. Materials Data on ErZn12 (SG:139) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  16. Final Project Report for ER15351 A Study of New Actinide Zintl Ion Materials

    SciTech Connect (OSTI)

    Peter K. Dorhout

    2007-11-12

    The structural chemistry of actinide main-group metal materials provides the fundamental basis for the understanding of structural coordination chemistry and the formation of materials with desired or predicted structural features. The main-group metal building blocks, comprising sulfur-group, phosphorous-group, or silicon-group elements, have shown versatility in oxidation state, coordination, and bonding preferences. These building blocks have allowed us to elucidate a series of structures that are unique to the actinide elements, although we can find structural relationships to transition metal and 4f-element materials. In the past year, we investigated controlled metathesis and self-propagating reactions between actinide metal halides and alkali metal salts of main-group metal chalcogenides such as K-P-S salts. Ternary plutonium thiophosphates have resulted from these reactions at low temperature in sealed ampules. we have also focused efforts to examine reactions of Th, U, and Pu halide salts with other alkali metal salts such as Na-Ge-S and Na-Si-Se and copper chloride to identify if self-propagating reactions may be used as a viable reaction to prepare new actinide materials and we prepared a series of U and Th copper chalcogenide materials. Magnetic measurements continued to be a focus of actinide materials prepared in our laboratory. We also contributed to the XANES work at Los Alamos by preparing materials for study and for comparison with environmental samples.

  17. Materials Data on ErSeI (SG:59) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  18. Materials Data on Er(SiPd)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. Materials Data on ErGaPd (SG:62) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-01-21

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. Materials Data on ErTlPd (SG:189) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  1. Materials Data on ErSiPd2 (SG:62) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-01-27

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Materials Data on Er2Pd2Pb (SG:127) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-19

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Materials Data on Er2CdS4 (SG:227) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Materials Data on Er10Ti6O27 (SG:8) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on Er(CoSi)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Final Report for DE-FG02-93ER14376,Ionic Transport in Electrochemical Media

    SciTech Connect (OSTI)

    J. W. Halley

    2009-05-20

    This project was a molecular dynamics study of the relevant issues associated with the structure and transport of lithium in polymer electrolytes such as polyethylene oxide(PEO). In close collaboration with quantum chemist Larry Curtiss and neutron scatterers David Lee Price and Marie-Louise Saboungi at Argonne, we used molecular dynamics to study the local structure and dynamics and ion transport in the polymer. The studies elucidated the mechanism of Li transport in PEO, revealing that the rate limiting step is extremely sensitive to the magnitude of the torsion forces in the backbone of the polymer. Because the torsion forces are difficult to manipulate chemically, this makes it easier to understand why improving the conductivity of PEO based electrolytes has proven to be very difficult. We studied the transport properties of cations in ionic liquids as possible additives to polymer membranes for batteries and fuel cells and found preliminary indications that the transport is enhanced near phase separation in acid-ionic liquid mixtures.

  7. Yearly Technical Report for DE-FG02-03ER46026

    SciTech Connect (OSTI)

    Duane D. Johnson

    2012-05-25

    We propose a unique, all-electron, thermodynamic density functional theory (DFT) code that directly predicts full or partial long-range order in crystalline (defected) solids and their effect on electronic properties via a first-principles mean-field theory, scales linear with number of atoms N per unit-cell [i.e. O(N), due to use of a mathematical-based screening in k-space], and addresses up to 1 million atoms using parallel architectures. Novel O(N) algorithms will be developed to permit this for an all-electron KKR Green's functional density-functional theory code.

  8. Materials Data on Er(NiGe)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  9. Materials Data on ErAl4Ni (SG:63) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  10. Materials Data on ErAl2Ni (SG:63) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. Materials Data on ErInNi (SG:189) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-01-21

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. Materials Data on ErNi7B3 (SG:141) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  13. Materials Data on Er2Ni17 (SG:194) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  14. Materials Data on ErHCl (SG:166) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2015-04-29

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  15. Materials Data on ErB2Ru3 (SG:191) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  16. Materials Data on Er(FeB)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  17. Materials Data on ErBRh3 (SG:221) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  18. Materials Data on ErFe4B (SG:191) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-05-16

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. BASIN VER DE GREAT ER ANETH BU G BAR KER DOME HOR SESH OE UTE DOME

    Gasoline and Diesel Fuel Update (EIA)

    BOE Reserve Class 0 20 40 10 30 Miles ± The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). The boundaries are not informed by subsurface structural information. The data and methods used in their creation are detailed in a report, "Scientific Inventory of Onshore

  20. BASIN VER DE GREAT ER ANETH BU G BAR KER DOME HOR SESH OE UTE DOME

    Gasoline and Diesel Fuel Update (EIA)

    Gas Reserve Class 0 20 40 10 30 Miles ± The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy Information Administration pursuant to studies required by Section 604 of the Energy Policy and Conservation Act Amendments of 2000 (P.L. 106-469). The boundaries are not informed by subsurface structural information. The data and methods used in their creation are detailed in a report, "Scientific Inventory of Onshore

  1. Final Technical Report for NSF/DOE partnership grant 1004284/ER54905/SC0004660; 2011- 2013

    SciTech Connect (OSTI)

    Stenzel, Reiner; Urrutia, Manuel

    2014-10-15

    Research has been performed on two topics: (1) Nonlinear time-dependent phenomena in the regime of electron magnetohydrodynamics (EMHD). (2) Plasma structures forming ”bubbles”. The latter are regions of localized density depletions or enhancements separated from the ambient plasma by sheaths or double layers. Three-dimensional magnetic null points can also be considered as ”magnetic bubbles”. Nonlinear EMHD fields are generated by pulsed magnetic loops. The magnetic field varies in time faster than an ion cyclotron period, hence interacts only with electrons. Space charge electric fields are created when magnetic forces try to separate electrons from ions. Electric and magnetic forces nearly balance to keep the plasma quasi neutral. This leads to rotational electron motions, i.e. Hall currents and associated magnetic perturbations. When the time scale falls between the ion and electron cyclotron period the magnetic perturbation is convected in the whistler mode. When the time-varying magnetic field exceeds the background magnetic field the whistler mode becomes highly nonlinear since its properties depend on the total magnetic field. When the time-varying magnetic field creates magnetic null points or null lines EMHD breaks down. Electrons can be accelerated in null lines which dissipated magnetic energy like in magnetic reconnection. Energized electrons with anisotropic distributions create secondary whistler instabilities. These effects have been observed in field topologies resembling spheromaks and field-reversed configurations (FRCs). The whistler mode propagation near magnetic nulls is not trivial when the field gradients and wavelength are comparable. Ray tracing is inappropriate when the WKB approximation breaks down. Experiments have been started to map wave propagation on curved field lines with null points. Initial results show that whistlers in highly nonuniform fields have highly oblique phase velocities but the energy flow remains dominantly along the field. Density bubbles are created by anode discharges, typically forming spherical ”fireballs” bounded by a double layer. In a magnetized plasma the shape becomes cylindrical, in nonuniform magnetic fields many asymmetric fireball shapes are possible. Due to electron energization the fireball plasma has higher density and temperature than the ambient plasma. The double layer can also be produced by a high transparency positively biased grid. Instabilities arise from the electron transit through the plasma bubble and the double layer. When a permanent magnet is biased positively the electrons perform ExB drifts in the equatorial plane between the poles. Electron drift modes are excited with a high spectral contents. Similarly, a negatively biased magnet produces a disk-shaped plasma bubble. The cold magnet emits secondary electrons due to the impact of energetic ions. Such magnetron discharges exhibit a variety of instabilities which affect the cross-field transport and sputtering applications. This report will summarize the main effects observed and provide references to more detailed publications.

  2. Closeout of the award DE-FG02-05ER46223. Trustees of the University...

    Office of Scientific and Technical Information (OSTI)

    University of Pennsylvania. Project title- "Modular Designed Protein Constructions for Solar Generated H2 From Water Dutton, P. Leslie Univ. of Pennsylvania,...

  3. Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic...

    Office of Scientific and Technical Information (OSTI)

    2007-2011 Genomic Structure, Metagenomics, Horizontal Gene Transfer, and Natural Diversity of Prochlorococcus ... Country of Publication: United States Language: English ...

  4. Materials Data on ErInAg2 (SG:225) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on ErAg2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. FINAL REPORT Plant Physiological Aspects of Silicon DE-FG03-94ER20151

    Office of Scientific and Technical Information (OSTI)

    .prepared as an account of work sponsored by an agency of the United States Government. ... of the nutrient salts used, and from the experimental environment and their own seeds. ...

  7. Laser Wakefield Acceleration: Structural and Dynamic Studies. Final Technical Report ER40954

    SciTech Connect (OSTI)

    Downer, Michael C.

    2014-12-19

    Particle accelerators enable scientists to study the fundamental structure of the universe, but have become the largest and most expensive of scientific instruments. In this project, we advanced the science and technology of laser-plasma accelerators, which are thousands of times smaller and less expensive than their conventional counterparts. In a laser-plasma accelerator, a powerful laser pulse exerts light pressure on an ionized gas, or plasma, thereby driving an electron density wave, which resembles the wake behind a boat. Electrostatic fields within this plasma wake reach tens of billions of volts per meter, fields far stronger than ordinary non-plasma matter (such as the matter that a conventional accelerator is made of) can withstand. Under the right conditions, stray electrons from the surrounding plasma become trapped within these wake-fields, surf them, and acquire energy much faster than is possible in a conventional accelerator. Laser-plasma accelerators thus might herald a new generation of compact, low-cost accelerators for future particle physics, x-ray and medical research. In this project, we made two major advances in the science of laser-plasma accelerators. The first of these was to accelerate electrons beyond 1 gigaelectronvolt (1 GeV) for the first time. In experimental results reported in Nature Communications in 2013, about 1 billion electrons were captured from a tenuous plasma (about 1/100 of atmosphere density) and accelerated to 2 GeV within about one inch, while maintaining less than 5% energy spread, and spreading out less than milliradian (i.e. millimeter per meter of travel). Low energy spread and high beam collimation are important for applications of accelerators as coherent x-ray sources or particle colliders. This advance was made possible by exploiting unique properties of the Texas Petawatt Laser, a powerful laser at the University of Texas at Austin that produces pulses of 150 femtoseconds (1 femtosecond is 10-15 seconds) in duration and 150 Joules in energy (equivalent to the muzzle energy of a small pistol bullet). This duration was well matched to the natural electron density oscillation period of plasma of 1/100 atmospheric density, enabling efficient excitation of a plasma wake, while this energy was sufficient to drive a high-amplitude wake of the right shape to produce an energetic, collimated electron beam. Continuing research is aimed at increasing electron energy even further, increasing the number of electrons captured and accelerated, and developing applications of the compact, multi-GeV accelerator as a coherent, hard x-ray source for materials science, biomedical imaging and homeland security applications. The second major advance under this project was to develop new methods of visualizing the laser-driven plasma wake structures that underlie laser-plasma accelerators. Visualizing these structures is essential to understanding, optimizing and scaling laser-plasma accelerators. Yet prior to work under this project, computer simulations based on estimated initial conditions were the sole source of detailed knowledge of the complex, evolving internal structure of laser-driven plasma wakes. In this project we developed and demonstrated a suite of optical visualization methods based on well-known methods such as holography, streak cameras, and coherence tomography, but adapted to the ultrafast, light-speed, microscopic world of laser-driven plasma wakes. Our methods output images of laser-driven plasma structures in a single laser shot. We first reported snapshots of low-amplitude laser wakes in Nature Physics in 2006. We subsequently reported images of high-amplitude laser-driven plasma bubbles, which are important for producing electron beams with low energy spread, in Physical Review Letters in 2010. More recently, we have figured out how to image laser-driven structures that change shape while propagating in a single laser shot. The latter techniques, which use the methods of computerized tomography, were demonstrated on test objects e.g. laser-driven filaments in air and glass and reported in Optics Letters in 2013 and Nature Communications in 2014. Their output is a multi-frame movie rather than a snapshot. Continuing research is aimed at applying these tomographic methods directly to evolving laser-driven plasma accelerator structures in our laboratory, then, once perfected, to exporting them to plasma-based accelerator laboratories around the world as standard in-line metrology instruments.

  8. Final Technical Report for DOE Award DE-FG02-05ER63959

    SciTech Connect (OSTI)

    Christopher S. Bretherton

    2011-04-08

    The goals of this work were: (1) to improve the University of Washington shallow cumulus parameterization, first developed by the PI's group for better simulation of shallow oceanic cumulus convection in the MM5 mesoscale model (Bretherton et al., 2004, Mon. Wea. Rev.); (2) to explore its applicability to deep (precipitating) cumulus convection; and (3) to explore fundamental physical issues related to this cumulus parameterization.

  9. Final Report for DOE contract DE-FG02-98ER62609

    SciTech Connect (OSTI)

    Webb, Thompson, III

    2012-08-20

    Several publications document, analyze, and map the paleoclimatic data available for the last glacial maximum (LGM) 21,000 years ago. Data coverage is fairly extensive for North America, Eurasia, and Africa. The data show much lower temperatures for that time in earth history. These data were used to check climate model simulations for the LGM. Simulations forced by the CLIMAP SST for the LGM, where the west/east SST gradient across the Pacific is reduced compared to present, produce a reduction in the strength of the trade winds and a decrease in the west/east slope of the equatorial thermocline that is incompatible with thermocline depths newly inferred from foraminiferal assemblages. Stronger than-present trade winds, and a more realistic simulation of the thermocline slope, are produced when eastern Pacific SSTs are 2oC cooler than western Pacific SSTs. Sensitivity experiments for the model simulations of the LGM were also used to show which aspects of the climate boundary conditions produced various changes in model simulations for the LGM. An experiment which has the LGM ice sheets for the Northern Hemisphere but no Eurasian ice sheet shows that the downstream cooling in Asia is largely dependent on sea-ice growth in the North Atlantic, which is a product of the Laurentide ice sheet. Weaker Asian monsoons develop as a result of this downstream cooling, which is largest for low Laurentide ice sheets of large areal extent.

  10. DOE-ER-46139-Phase II-Final-Report-Tritt-2011

    SciTech Connect (OSTI)

    Terry M. Tritt

    2011-10-21

    This proposal emphasizes investigations of the thermal and electrical transport properties of new and novel solid-state materials, with the specific goal of achieving higher efficiency solid-state thermoelectric materials. This program will continue to build a very strong collaborative research effort between researchers at Oak Ridge National Laboratory (ONRL) and Clemson University. We propose three new faculty hires and major equipment purchases in order to further enhance our level of national recognition. We will be positioned for competition for major non-EPSCoR DOE and DOD funding (i.e. NSF-Materials Research Center) and able to address many other areas of DOE and national importance. Graduate and undergraduate students will be extensively involved in this project, spending significant time at ORNL, thus gaining important training and educational opportunities. We will also include an outreach program to bring in outside students and faculty. An External Advisory Board of distinguished scientists will provide oversight to the program.

  11. OF TilE ADMINISTRATOR OF THE BONNEVILLE POl\\'ER ADMINISTRATION

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    well in excess of 10 percent as an emergency measure. Following the extreme low-water period in the fall months of 1942, added demandt by war industry throughout the...

  12. Award ER25844: Minimizing System Noise Effects for Extreme-Scale Scientific Simulation Through Function Delegation

    SciTech Connect (OSTI)

    Lumsdaine, Andrew

    2012-11-20

    In software running on distributed computing clusters, time spent on communication between nodes in the cluster can be a significant portion of the overall computation time; background operating system tasks and other computational ?noise? on the nodes of the system can have a significant impact on the amount of time this communication takes, especially on large systems. The research completed in this period has improved understanding of when such noise will have a significant impact. Specifically, it was demonstrated that not just noise on the nodes, but also noise on the network between nodes can have a significant impact on computation time. It was also demonstrated that noise patterns matter more than noise intensity: very regular noise can cause less disruption than lighter (on average) but less regular noise. It was also demonstrated that the effect of noise is more prominent as the speed of the network between nodes is increased. Furthermore, a tracing tool, Netgauge, was improved via our work, and a system simulator, LogGOPSim, was developed; they can be used by application developers to improve performance of their program and by system designers to mitigate the effects of noise by adjusting the noise characteristics of the operating system. Both have been made freely available as open source programs. In the course of developing these tools, we demonstrated weaknesses in existing methodologies for modeling communication, and we introduced a more detailed model, LogGOPS, for simulating systems. Not only were the deleterious effects of noise explored but we have also offered solutions. Our studies of simulations of system noise have led to specific recommendations on tuning systems to mitigate noise. We have also improved existing approaches to mitigating noise. ?Non-blocking collective communication? avoids the effects of noise by letting communication continue simultaneously with computation (thus being ?non-blocking?), so that the delays in communication introduced by noise have a smaller impact on overall computation time. Potentially, noise can be reduced much further by ?offloading? communication tasks to a separate processing element than the operating system is using. We have improved our library LibNBC, which provides an implementation of non-blocking collectives, via this work. During this research, our proposal to include non-blocking collectives (which used LibNBC as a reference implementation) in the upcoming MPI-3 standard was accepted. As MPI is a ubiquitous and important standard for communication in parallel computing, this demonstrates a certain acceptance of the practicality and desirability of non-blocking collectives. Now that non-blocking collectives are a part of the standard we can expect to see optimized platform-specific implementations of non-blocking collectives. Also as part of this work we have also developed a language GOAL (Global Operation Assembly Language) that can be used as a starting point for defining languages to express optimized communication algorithms.

  13. Controlling n-Type Carrier Density from Er Doping of InGaAs with...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: English Subject: solar (photovoltaic), solid state lighting, phonons, thermoelectric, bio-inspired, energy storage (including ...

  14. FINAL REPORT DOE Grant: DE-FG02-89ER45391

    SciTech Connect (OSTI)

    James R. Chelikowsky

    2009-02-27

    The work reported here took place at the University of Minnesota from 07/01/1989 to 06/30/2006. Most of this work focused on computational materials applied to oxides during the first part of this funding period and to nanoscale materials toward the end of the funding period. This funding resulted in three monographs, 36 invited articles or book chapters, 160 articles in refereed journals and 89 invited talks. The funding helped train 13 PhD students and one masters student. The PI received two national research awards for this work. A list of these contributions are presented.

  15. Final report for DOE grant FG02-06ER15805

    SciTech Connect (OSTI)

    Daniel Gage

    2012-05-31

    DOE funding was used to investigate the role of the phosphotransferase system (PTS) in the symbiotic, nodulating bacterium Sinorhizobium meliloti. This system is well studied in several bacterial species. However, it??s organization and function in S. meliloti is substantially different than in the those other, well-studied bacteria. The S. meliloti PTS, through our DOE-funded work, has become a model for how this important signal transduction system works in the a-proteobacteria. We have found that the PTS is relatively simple, used for only signal transduction and not transport, and is involved in regulation of carbon metabolism in response to carbon availability and nitrogen availability.

  16. Audit of Groundwater Remediation Plans at Savannah River, ER-B-96-02

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    U.S. DEPARTMENT OF ENERGY OFFICE OF INSPECTOR GENERAL AUDIT OF GROUNDWATER REMEDIATION PLANS AT THE SAVANNAH RIVER SITE The Office of Inspector General wants to make the distribution of its reports as customer friendly and cost effective as possible. Therefore, this report will be available electronically through the Internet five to seven days after publication at the following alternative addresses: Department of Energy Headquarters Gopher gopher.hr.doe.gov Department of Energy Headquarters

  17. DE-FG02-04ER25606 Identity Federation and Policy Management Guide: Final Report

    SciTech Connect (OSTI)

    Humphrey, Marty, A

    2011-05-25

    The goal of this 3-year project was to facilitate a more productive dynamic matching between resource providers and resource consumers in Grid environments by explicitly specifying policies. There were broadly two problems being addressed by this project. First, there was a lack of an Open Grid Services Architecture (OGSA)-compliant mechanism for expressing, storing and retrieving user policies and Virtual Organization (VO) policies. Second, there was a lack of tools to resolve and enforce policies in the Open Services Grid Architecture. To address these problems, our overall approach in this project was to make all policies explicit (e.g., virtual organization policies, resource provider policies, resource consumer policies), thereby facilitating policy matching and policy negotiation. Policies defined on a per-user basis were created, held, and updated in MyPolMan, thereby providing a Grid user to centralize (where appropriate) and manage his/her policies. Organizationally, the corresponding service was VOPolMan, in which the policies of the Virtual Organization are expressed, managed, and dynamically consulted. Overall, we successfully defined, prototyped, and evaluated policy-based resource management and access control for OGSA-based Grids. This DOE project partially supported 17 peer-reviewed publications on a number of different topics: General security for Grids, credential management, Web services/OGSA/OGSI, policy-based grid authorization (for remote execution and for access to information), policy-directed Grid data movement/placement, policies for large-scale virtual organizations, and large-scale policy-aware grid architectures. In addition to supporting the PI, this project partially supported the training of 5 PhD students.

  18. Progress Report for DOE FG02-08ER64510 (Final, April 2014)

    SciTech Connect (OSTI)

    Dunn, Robert

    2014-04-01

    Over the course of five years we have established a long-term array of warming chambers at Duke and Harvard Forest that simulate future conditions with regard to temperature. In these chambers, we have studied, ants, other animal taxa, fungi, bacteria and plants and their responses to the treatments. We have coupled these studies with lab experiments, large-scale observations, and models to contextualize our results. Finally, we have developed integrative models of the future distribution of species and their consequences as a result of warming in eastern North America and more generally.

  19. Materials Data on Er2(SeO3)3 (SG:2) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. Materials Data on Er4(Al8Pt3)3 (SG:2) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  1. Report/Product Number(s) DOE/ER/64701 DOE Award/Contract Number...

    Office of Scientific and Technical Information (OSTI)

    ... J. 8:316-33. Michael L. Robbins, Ansuman Roy, Po-Hao Wang, Iffa Gaffoor, Rajandeep S. Sekhon, Marcia M. de O. Buanafina, Jai S. Rohila, Surinder Chopra (2013). Comparative ...

  2. Materials Data on NaEr(PS3)2 (SG:2) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Materials Data on Er(NiGe)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Materials Data on ErNbO4 (SG:15) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on Er2C(NO)2 (SG:164) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on Er2SO2 (SG:164) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. Final Report: DOE award: ER64516-1031199-0013966 2007-2011 Genomic

    Office of Scientific and Technical Information (OSTI)

    interface. That is, we wanted to develop our understanding of the biology of these microbes at all scales of biological organization, from individual cell design to the dynamics...

  8. ERS 14.1 Satellite Accumulation Ares (RCRA Compliance), 4/30/13

    Broader source: Energy.gov [DOE]

    The objective of this surveillance is to evaluate the effectiveness of the contractor's management of hazardous and mixed wastes in satellite accumulation areas.  The Facility Representative...

  9. Materials Data on Er3Pb4F17 (SG:1) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  10. Final Technical Report for DOE Grant DE-FG02-02ER83371, Phase II

    SciTech Connect (OSTI)

    Townsend, William; Wilkinson, David; Hamel, William; Zhou, Renbin; Nycz, Andrzej; Humphreys, Heather

    2006-04-14

    The purpose of this research was to develop a telerobotic master device consisting of a 7-axis backdrivable robotic arm, and a pressure-sensitive grip-controller integrated with a Compact Remote Console (CRC), thus creating a highly functional teleoperation station targeted to control a 6-axis industrial robotic arm and dexterous robotic hand to be used for demolition work in a nuclear setting. We successfully completed the development of one of the world?s smallest brushless motor controllers due partially to funding through this grant. These controllers are used to drive the motors in the master robotic arm. We also completed the development of an improved model of a highly advanced 4 degree-of-freedom arm ? this same arm is the core component in the teleoperation system. The WAM arm and a 3-axis gimbals were integrated with a commercially available CRC at our consultant?s lab at University of Tennessee. Additional support hardware and software were combined to tie the master control system to an existing industrial robot in the lab. A master controller for a dexterous hand was developed and became an integral part of the gimbals handle. Control algorithms were developed and the software was written and implemented. The entire system was then debugged and tested. Results of the prototype system are promising. The WAM Arm, gimbals, hand controller and CRC were successful integrated. Testing of the system to control the 6-axis industrial arm and prototype dexterous hand showed great potential. Relatively simple tasks were successfully performed at slow speeds. Some of the testing was hampered by problems with the slave dexterous hand. This is a prototype hand being developed by Barrett under a different Phase II program. Potential improvements and advancements to the system include improving the control code, and integration of a 2nd master controller arm in order to drive a 2nd slave arm and hand. In summary, the device is a complex system with advanced features and could be used as a universal platform for efficient controlling of robotic arms performing remote tasks in unstructured and uncertain environments such as those prevalent in environmental clean up.

  11. Materials Data on Er(FeSn)6 (SG:63) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. Materials Data on Er(BC)2 (SG:127) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2015-03-08

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  13. Materials Data on Er2Be2GeO7 (SG:113) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  14. McClean-DE-FG02-05ER64119_final

    Office of Scientific and Technical Information (OSTI)

    ... in a model where ocean flows were better resolved than in standard resolution models. ... and high-resolution modeling to the teachers, particularly sharing visualizations of ...

  15. Final Report for the grant "Applied Geometry" (DOE DE-FG02-04ER25657)

    SciTech Connect (OSTI)

    Prof. Mathieu Desbrun

    2009-05-20

    The primary purpose of this 3-year DOE-funded research effort, now completed, was to develop consistent, theoretical foundations of computations on discrete geometry, to realize the promise of predictive and scalable management of large geometric datasets as handled routinely in applied sciences. Geometry (be it simple 3D shapes or higher dimensional manifolds) is indeed a central and challenging issue from the modeling and computational perspective in several sciences such as mechanics, biology, molecular dynamics, geophysics, as well as engineering. From digital maps of our world, virtual car crash simulation, predictive animation of carbon nano-tubes, to trajectory design of space missions, knowing how to process and animate digital geometry is key in many cross-disciplinary research areas.

  16. Final report for DE-FG02-95ER40898

    SciTech Connect (OSTI)

    Starkman, Glenn David; Mathur, Harsh

    2013-07-20

    PI G. Starkman and Co-I H. Mathur report on the past three years of their research at the frontiers of particle physics and cosmology.

  17. Plant training grant: DE-FG02-94ER20162. Final technical report

    SciTech Connect (OSTI)

    Cashmore, Anthony R.

    2003-02-01

    The aim of this training grant was to educate students of Plant Science in the disciplines of Biochemistry and Chemistry, in addition to the more traditional courses in Plant Biology. Annual retreats were held which involved a day-long meeting and included lectures from Penn faculty as well as famous national and international scientists. Programs for two of these retreats are included. In addition to lecture courses, students performed research within the Departments of Biology, Chemistry, and Biochemistry and Biophysics; a publications list is given.

  18. Materials Data on Er2C(NO)2 (SG:164) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. Materials Data on ErNbO4 (SG:15) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. Materials Data on Er2SO2 (SG:164) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  1. Materials Data on Er2Ru2O7 (SG:227) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Combined Final Technical Report 04ER86191 H2Cryo.pdf

    Office of Scientific and Technical Information (OSTI)

    ... REFERENCES 1. Masur L. J. et al., IEEE Trans. Appl. Sup., V. 11 (2001), p. 3256. 2. Fischer K. et al., IEEE Trans. Appl. Sup., V. 9 (1999), p. 2625. 3. L. F. Goodrich and T. C. ...

  3. Progress report for DE_FC02-06ER25786 (TOPS2)

    Office of Scientific and Technical Information (OSTI)

    ... Technical Report UCBEECS-2007-123, October 2007 Short version appeared in IEEE Intern. ... be performed quickly, partial support for IEEE floating point arithmetic) make it ...

  4. DOE Grant DEFG02-95ER25253 Final Report Development of Simulation...

    Office of Scientific and Technical Information (OSTI)

    ... has been conducted, in collaboration with Garcea, on developing non-kinetic models of papovaviruses, a class of animal viruses including some believed to cause cancer in humans. ...

  5. Microsoft Word - Final Technical Report DE-FG02-07ER64399 Tien...

    Office of Scientific and Technical Information (OSTI)

    ... We were also able to: i) developed methodology to isolate sufficient RNA from a limited ... under Prolonged Electron Acceptor Limitation" Journal of Proteome Research (in press)

  6. Materials Data on Er(IO3)3 (SG:14) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. Microsoft Word - ARM report final DE-FG02-05ER63954.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary of Research (Final Technical Report) Project Title: "Development and application of new methods to retrieve vertical structure of precipitation above the ARM CART sites from MMCR measurements" Type of report: Summary of Research (Final Technical Report) PI: Dr. Sergey Matrosov Period covered: 12/01/2004 - 11/30/2010 Institution: Cooperative Institute for Research in Environmental Sciences (CIRES), University of Colorado, Campus Box 216, Boulder, CO 80309-0216 Grant number:

  8. Materials Data on Sr(ErS2)2 (SG:122) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  9. Final Report for Project DE-FC02-06ER25755 [Pmodels2

    SciTech Connect (OSTI)

    Panda, Dhabaleswar; Sadayappan, P

    2014-03-12

    In this report, we describe the research accomplished by the OSU team under the Pmodels2 project. The team has worked on various angles: designing high performance MPI implementations on modern networking technologies (Mellanox InfiniBand (including the new ConnectX2 architecture and Quad Data Rate), QLogic InfiniPath, the emerging 10GigE/iWARP and RDMA over Converged Enhanced Ethernet (RoCE) and Obsidian IB-WAN), studying MPI scalability issues for multi-thousand node clusters using XRC transport, scalable job start-up, dynamic process management support, efficient one-sided communication, protocol offloading and designing scalable collective communication libraries for emerging multi-core architectures. New designs conforming to the Argonne’s Nemesis interface have also been carried out. All of these above solutions have been integrated into the open-source MVAPICH/MVAPICH2 software. This software is currently being used by more than 2,100 organizations worldwide (in 71 countries). As of January ’14, more than 200,000 downloads have taken place from the OSU Web site. In addition, many InfiniBand vendors, server vendors, system integrators and Linux distributors have been incorporating MVAPICH/MVAPICH2 into their software stacks and distributing it. Several InfiniBand systems using MVAPICH/MVAPICH2 have obtained positions in the TOP500 ranking of supercomputers in the world. The latest November ’13 ranking include the following systems: 7th ranked Stampede system at TACC with 462,462 cores; 11th ranked Tsubame 2.5 system at Tokyo Institute of Technology with 74,358 cores; 16th ranked Pleiades system at NASA with 81,920 cores; Work on PGAS models has proceeded on multiple directions. The Scioto framework, which supports taskparallelism in one-sided and global-view parallel programming, has been extended to allow multi-processor tasks that are executed by processor groups. A quantum Monte Carlo application is being ported onto the extended Scioto framework. A public release of Global Trees (GT) has been made, along with the Global Chunks (GC) framework on which GT is built. The Global Chunks (GC) layer is also being used as the basis for the development of a higher level Global Graphs (GG) layer. The Global Graphs (GG) system will provide a global address space view of distributed graph data structures on distributed memory systems.

  10. BASIN VER DE GREAT ER ANETH BU G BAR KER DOME HOR SESH OE UTE...

    U.S. Energy Information Administration (EIA) Indexed Site

    Liquids Reserve Class 0 20 40 10 30 Miles The mapped oil and gas field boundary outlines were created by the Reserves and Production Division, Office of Oil and Gas, Energy ...

  11. Materials Data on ErP (SG:225) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. Y-12ers earn degrees through Friday Academy | Y-12 National Security...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    1:21pm Print version Debra Avery, Mark Burdettte, Michael Fierley, Jessica Griffin, Gary Lewis, Christine Stalnaker, and Ben Stephens. Not pictured Jeffrey Smith. Photo by Kathy...

  13. Scientific and engineering services for the LANCE/ER accelerator production of tritium (APT) project

    SciTech Connect (OSTI)

    1994-12-05

    The APT project office is conducting a preconceptual design study for an accelerator driven concept to produce tritium. The facility will require new technology in many areas, since the scale of this accelerator is significantly larger then any in operation to date. The facility is composed of four subsystems: accelerator, target & blanket, balance of plant, and tritium purification system (TPS). New physics realms will be entered in order for the concept to be feasible; for example, extremely high energy levels of the entering protons that induce (multiplicative) spallation of the neutrons from the high Z target will occur. These are complex and require advance codes (MCNP) to predict the physics interactions and as well as deleterious material effects in the surrounding structures. Other issues include component cooling and complex thermal-hydraulics effects within the blanket and the beam {open_quotes}window.{close_quotes} In order to support a DOE mandated fast ROD schedule, Los Alamos APT staff will be provided with senior, engineering technical support staff with direct APT technology experience and whom are {open_quotes}on site{close_quotes}. This report contains resumes of the staff.

  14. Audit Report: ER-B-97-02 | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of the Department of Energy's Grant for Economic Development at the Mound Plant The downsizing of the Department of Energy's (Department) facilities as a result of the end of the...

  15. Materials Data on Er2FeSi2 (SG:12) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-19

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  16. Materials Data on ErIr (SG:221) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2015-03-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  17. Materials Data on Er(SiIr)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  18. DE-FG02-04ER63782_FinalReport

    Office of Scientific and Technical Information (OSTI)

    ... The amount of agar in each miniature chamber is minimal (around 1 microliter), which allows for quality microscopy observations of grown material prior to removalisolation. The ...

  19. Final Report DOE Award # DE-FG02-04ER63923

    Office of Scientific and Technical Information (OSTI)

    ... Vesicle formation associated with Se reduction. Desulfitobacterium hafniense DCB-2 grew in minimal medium with selenate concentrations up to 25 mM, and reduced both selenate and ...

  20. DOE Award Number DE-FG03-95ER40906

    Office of Scientific and Technical Information (OSTI)

    ... are received from the journal referees and have been ... Arizona provides project management as well. John ... development and much of the R&D. But because the physicists ...

  1. Materials Data on Er(CoSi)2 (SG:139) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Final Report for DE-FG02-00ER41145

    SciTech Connect (OSTI)

    LoSecco, John

    2013-06-28

    Research in high energy physics has been conducted under this research contract. The research includes the discovery of CP violation in the decays of B mesons and the determination of the CKM mechanism as the source of all CP violation in hadrons. Searches for physics beyond the standard model has resulted in limits from the B to S gamma transition and the discovery of the intermediate range oscillations of electron neutrinos. This last discovery will enable the discovery of all properties of neutrino mixing in the next round of neutrino experiments.

  3. Final Report for Award DE-FG02-09ER64721

    SciTech Connect (OSTI)

    somerville, chris

    2014-04-26

    The original goal of this award was to develop a proteoglycan chip containing suitable oligosaccharides that could be used as substrates for glycosyltransferases involved in synthesis or proteoglycans in higher plant cell walls. We had previously developed a suite of cloned enzymes that could be used to cleave most of the relevant glycosidic linkages in plant cell walls. The next step, supported by the previous award and this award, was to produce a series of transgenic plants in which synthetic proteins were introduced that contained each of the known sequence motifs that induce prolyl hydroxylation, and subsequent glycosylation. This work was completed and published in Estevez et al (2006). We then engaged on a series of experiments to define the properties of the prolyl hydroxylases that convert certain prolyl resides to hydroxyproline for subsequent glycosylation. This proved to be a challenging goal that required recruitment of an international team of complementary skills and several additional years or research. However, the effort was successful and has been published in Science recenty (Velasquez et al., 2011). In the course of this project, the postdoc supported by the award (Jose Estevez) was asked to provide technical assistance to a colleague at Stanford because of his expertise in marine polysaccharides. This led to the important discovery that marine algae have compounds that could be classified as lignin (Martone et al., 2009). Publications supported by the award Estvez, J.M., Kieliszewski, M.J., Khitrov, N., Somerville, C. (2006) Characterization of synthetic hydroxyproline-rich proteoglycans with AGP- and extensin-motifs in Arabidopsis. Plant Physiol., 142,458-470 Martone, P.T., Estevez, J.M., Lu, F., Ruel, K., Ralph, J., Denny, M.W., Somerville, C.R. (2009) Discovery of lignin in seaweed reveals convergent evolution of cell-wall architecture. Curr. Biol., 19, 169-175 Velasquez, S.M., M. Ricardi, M.M., Dorosz, J.G., Fernandez, P.V., Nadra, A.D., Pol-Fachin, L., Egelund, J., Gille, S., Harholt, J., Ciancia, M., Verli, H., Pauly, M., Bacic, A., Olsen, C.E., r Ulvskov, P., Petersen, B.L., Somerville, C., Iusem, N.D. & Estevez, J.M. (2011) O-glycosylated cell wall proteins are essential in root hair growth. Science 332,1401-1403

  4. Final Report for Grant DE-FG02-90ER61072

    SciTech Connect (OSTI)

    Lee Harrison

    2006-02-20

    This is the final report for the work done by our research group at the Atmospheric Sciences Research Center for the US DOE Atmopheric Radiation Measurement (ARM) Program. We were involved from the beginning of the ARM effort; we designed the Multi-filter Rotating Shadowband Spectroradiometer (MFRSR) which was widely deployed (and still operational in ARM) and through the years did a wide variety of data analysis on the returned data from these instruments. We also developed the Rotating Shadowband Spectroradiometer, which ARM deployed and also still deploys. Many scientific papers have been written using the data from these instruments, and the ongoing data streams remain part of the current ARM effort. Earlier reports contain our progress from previous grant periods, this report covers the last period and provides references to published work.

  5. Final technical Report DE-FG02-06ER65187

    SciTech Connect (OSTI)

    Edwin Eloranta

    2009-07-17

    Simulations from the University of Wisconsin Non-Hydrostatic Modeling System (UW-NMS) along with those from other models indicate a strong tendency to overproduce ice, resulting in a decimation of the liquid portion of mixed-phase stratus through the Bergeron-Findeissen process. Immersion freezing was illustrated to be a major contributor to ice production within these cloud layers, and aerosol properties were illustrated to be an important consideration in the simulation of this process. In particular, the soluble mass fraction and aerosol insoluble mass type were demonstrated to influence simulation of the immersion freezing process, Data collected by the Arctic High Spectral Resolution Lidar and Millimeter Cloud Radar during the M-PACE period was analyzed in order to provide a statistical dataset for validation of simulations of mixed-phase stratus. 270 hours of single-layer cases were reviewed, and mean values for cloud base height, cloud thickness, cloud optical thickness, cloud temperature, wind direction, and liquid and ice particle size, particle number density, and water content were derived.

  6. Microsoft Word - DOE-ER-0670T_6.09_Final.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Within the entire global change research program, ARM represents the only opportunity to bring atmospheric radiation measurements, and hence models, to the level of quality needed ...

  7. Final Report for Grant No. DE-FG02-10ER15527 Edgar P. Spalding...

    Office of Scientific and Technical Information (OSTI)

    Interacting glutamate receptor-like proteins in phloem regulate lateral root initiation. ... A review of Ca2+ transport was also published, acknowledging exclusive funding by the DOE ...

  8. Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenal

    SciTech Connect (OSTI)

    Tsirulnikov, Kirill; Abuladze, Natalia; Bragin, Anatol; Brain Research Institute, University of California at Los Angeles, CA 90095 ; Faull, Kym; Department of Psychiatry and Biobehavioral Sciences, University of California at Los Angeles, CA 90095; Pasarow Mass Spectrometry Laboratory, University of California at Los Angeles, CA 90095 ; Cascio, Duilio; Damoiseaux, Robert; Schibler, Matthew J.; Pushkin, Alexander

    2012-09-15

    4-Hydroxy-2-nonenal (4HNE) and acrolein (ACR) are highly reactive neurotoxic products of lipid peroxidation that are implicated in the pathogenesis and progression of Alzheimer's and Parkinson's diseases. Conjugation with glutathione (GSH) initiates the 4HNE and ACR detoxification pathway, which generates the mercapturates of 4HNE and ACR that can be excreted. Prior work has shown that the efficiency of the GSH-dependent renal detoxification of haloalkene derived mercapturates is significantly decreased upon their deacetylation because of rapid transformation of the deacetylated products into toxic compounds mediated by ?-lyase. The enzymes of the GSH-conjugation pathway and ?-lyases are expressed in the brain, and we hypothesized that a similar toxicity mechanism may be initiated in the brain by the deacetylation of 4HNE- and ACR-mercapturate. The present study was performed to identify an enzyme(s) involved in 4HNE- and ACR-mercapturate deacetylation, characterize the brain expression of this enzyme and determine whether its inhibition decreases 4HNE and 4HNE-mercapturate neurotoxicity. We demonstrated that of two candidate deacetylases, aminoacylases 1 (AA1) and 3 (AA3), only AA3 efficiently deacetylates both 4HNE- and ACR-mercapturate. AA3 was further localized to neurons and blood vessels. Using a small molecule screen we generated high-affinity AA3 inhibitors. Two of them completely protected rat brain cortex neurons expressing AA3 from the toxicity of 4HNE-mercapturate. 4HNE-cysteine (4HNE-Cys) was also neurotoxic and its toxicity was mostly prevented by a ?-lyase inhibitor, aminooxyacetate. The results suggest that the AA3 mediated deacetylation of 4HNE-mercapturate may be involved in the neurotoxicity of 4HNE.

  9. Low doses of ochratoxin A upregulate the protein expression of organic anion transporters Oat1, Oat2, Oat3 and Oat5 in rat kidney cortex

    SciTech Connect (OSTI)

    Zlender, Vilim; Breljak, Davorka; Ljubojevic, Marija; Flajs, Dubravka; Balen, Daniela; Brzica, Hrvoje; Domijan, Ana-Marija; Peraica, Maja; Fuchs, Radovan; Anzai, Naohiko; Sabolic, Ivan

    2009-09-15

    Mycotoxin ochratoxin A (OTA) is nephrotoxic in various animal species. In rodents, OTA intoxication impairs various proximal tubule (PT) functions, including secretion of p-aminohippurate (PAH), possibly via affecting the renal organic anion (OA) transporters (Oat). However, an effect of OTA on the activity/expression of specific Oats in the mammalian kidney has not been reported. In this work, male rats were gavaged various doses of OTA every 2nd day for 10 days, and in their kidneys we studied: tubule integrity by microscopy, abundance of basolateral (rOat1, rOat3) and brush-border (rOat2, rOat5) rOat proteins by immunochemical methods, and expression of rOats mRNA by RT-PCR. The OTA treatment caused: a) dose-dependent damage of the cells in S3 segments of medullary rays, b) dual effect upon rOats in PT: low doses (50-250 {mu}g OTA/kg b.m.) upregulated the abundance of all rOats, while a high dose (500 {mu}g OTA/kg b.m.) downregulated the abundance of rOat1, and c) unchanged mRNA expression for all rOats at low OTA doses, and its downregulation at high OTA dose. Changes in the expression of renal Oats were associated with enhanced OTA accumulation in tissue and excretion in urine, whereas the indicators of oxidative stress either remained unchanged (malondialdehyde, glutathione, 8-hydroxydeoxyguanosine) or became deranged (microtubules). While OTA accumulation and downregulation of rOats in the kidney are consistent with the previously reported impaired renal PAH secretion in rodents intoxicated with high OTA doses, the post-transcriptional upregulation of Oats at low OTA doses may contribute to OTA accumulation and development of nephrotoxicity.

  10. LAKESHORE AVON BR ANT-EDEN ALD EN-LANC ASTER AU BURN W SH ELDON

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    81 § ¨ ¦ 81 LAKESHORE AVON BR ANT-EDEN ALD EN-LANC ASTER AU BURN W SH ELDON CALEDONIA HURON C REEK LEIC EST ER COL DEN ASH FORD INDIAN FALLS LAWTONS SAR DINIA RPD-037 -2 GLENWOOD PU LASKI PAVILION CON CORD COL LINS N ELM A ORC HARD PARK-H AMBU RG DANLEY CORNERS ST ILLWAT ER CHAFF EE-ARCAD E FAYETT E-WATERLOO LAKEVIEW JAVA SEN EC A W ELLER Y AU RORA E ZOAR BU FFALO TIOGA SILVER LAKE AKR ON ROM E RAT HBON E ALM A BET HANY WYOMING ULYSSES BR ANCH W SAN DY CREEK COL LINS BLOOMFIELD E LEBANON

  11. Role of the nuclear xenobiotic receptors CAR and PXR in induction of cytochromes P450 by non-dioxinlike polychlorinated biphenyls in cultured rat hepatocytes

    SciTech Connect (OSTI)

    Ghrs, Maike; Roos, Robert; Andersson, Patrik L.; Schrenk, Dieter

    2013-10-01

    Polychlorinated biphenyls (PCBs) are among the most ubiquitously detectable persistent organic pollutants. In contrast to dioxinlike (DL) PCBs, less is known about the molecular mode of action of the larger group of the non-dioxinlike (NDL) PCBs. Owing to the life-long exposure of the human population, a carcinogenic, i.e., tumor-promoting potency of NDL-PCBs has to be considered in human risk assessment. A major problem in risk assessment of NDL-PCBs is dioxin-like impurities that can occur in commercially available NDL-PCB standards. In the present study, we analyzed the induction of CYP2B1 and CYP3A1 in primary rat hepatocytes using a number of highly purified NDL-PCBs with various degrees of chlorination and substitution patterns. Induction of these enzymes is mediated by the nuclear xenobiotic receptors CAR (Constitutive androstane receptor) and PXR (Pregnane X receptor). For CYP2B1 induction, concentrationresponse analysis revealed a very narrow window of EC{sub 50} estimates, being in the range of 14 ?M for PCBs 28 and 52, and between 0.4 and 1 ?M for PCBs 101, 138, 153 and 180. CYP3A1 induction was less sensitive to NDL-PCBs, the most pronounced induction being achieved at 100 ?M with the higher chlorinated congeners. Using okadaic acid and small interfering RNAs targeting CAR and PXR, we could demonstrate that CAR plays a major role and PXR a minor role in NDL-PCB-driven induction of CYPs, both effects showing no stringent structureactivity relationship. As the only obvious relevant determinant, the degree of chlorination was found to be positively correlated with the inducing potency of the congeners. - Highlights: We analyzed six highly purified NDL-PCBs for CYP2B1 and CYP3A1 expression. CAR plays a major, PXR a minor role in NDL-PCB-driven induction of CYPs. The degree of chlorination seems to be the major parameter for the inducing potency. There exists a competition between CAR and PXR. Activated PXR may antagonize CAR binding to the CYP2B1 promoter.

  12. Apelin-13-induced proliferation and migration induced of rat vascular smooth muscle cells is mediated by the upregulation of Egr-1

    SciTech Connect (OSTI)

    Liu, Qi-Feng; Yu, Hong-Wei; You, Lu; Liu, Ming-Xin; Li, Ke-Yan; Department of Cardiology, Jinzhou Central Hospital, Jinzhou 121001 ; Tao, Gui-Zhou

    2013-09-20

    Highlights: The mechanism underlying the effects of Apelin-13 on VSMC was investigated. Apelin-13 induced VSMC migration, proliferation and Egr-1 and OPN upregulation. These effects were inhibited by the Egr-1 specific deoxyribozyme, ED5. The effects of Apelin-13 on VSMC are mediated via Egr-1 upregulation. These data will help in attempts to prevent and treat vascular remodeling diseases. -- Abstract: Apelin-13 plays an important role in the migration and proliferation of vascular smooth muscle cells (VSMCs); however, the underlying mechanisms are still unclear. Egr-1 is a nuclear transcription factor, which is considered to be the critical initiating factor of the processes of VSMC proliferation and migration. Egr-1 is known to regulate the expression of osteopontin (OPN), which is a marker of the phenotypic modulation that is a necessary condition of VSMC proliferation and migration. We hypothesized that the role of Apelin-13 is mediated via upregulation of Egr-1. To test this hypothesis, we analyzed the effects of Apelin-13 treatment on Egr-1 mRNA and protein expression in A10 rat aortic VSMCs by RT-PCR and Western blotting, respectively. Results showed that, Apelin-13 upregulated the expression of Egr-1. Furthermore, treatment with the extracellular-regulated protein kinase (ERK) inhibitor, PD98059, inhibited the upregulation of Egr-1 by Apelin-13. In addition, this upregulation was inhibited by treatment of VSMCs with the Egr-1 specific deoxyribozyme ED5 (DNAenzyme/10-23 DRz). Furthermore, ED5 treatment was found to significantly inhibit Apelin-13-induced migration and proliferation of VSMCs using transwell and MTT assays, respectively. The evaluation of OPN mRNA and protein expression levels by RT-PCR and Western blot analyses revealed that ED5 treatment also inhibited Apelin-13-induced OPN upregulation. The results of this study indicated that Apelin-13 upregulates Egr-1 via ERK. Furthermore, Apelin-13 induced the proliferation and migration of VSMCs as well as the upregulation of OPN via the upregulation of Egr-1. These results will provide an important theoretical and experimental basis for the control of inappropriate remodeling of vessel walls, and will hopefully lead to the prevention and treatment of vascular remodeling diseases.

  13. Experiments to Understand HPC Time to Development (Final report for Department of Energy contract DE-FG02-04ER25633) Report DOE/ER/25633-1

    SciTech Connect (OSTI)

    Basili, Victor, R.; ,; Zelkowitz, Marvin, V.

    2007-11-14

    In order to understand how high performance computing (HPC) programs are developed, a series of experiments, using students in graduate level HPC classes and various research centers, were conducted at various locations in the US. In this report, we discuss this research, give some of the early results of those experiments, and describe a web-based Experiment Manager we are developing that allows us to run studies more easily and consistently at universities and laboratories, allowing us to generate results that more accurately reflect the process of building HPC programs.

  14. DAiSES: Dynamic Adaptivity in Support of Extreme Scale Department of Energy Project No. ER25622 Prime Contract No. DE-FG02-04ER25622 Final Report for September 15, 2004-September 14, 2008

    SciTech Connect (OSTI)

    PI: Patricia J. Teller, Ph.D.

    2009-05-05

    The DAiSES project [Te04] was focused on enabling conventional operating systems, in particular, those running on extreme scale systems, to dynamically customize system resource management in order to offer applications the best possible environment in which to execute. Such dynamic adaptation allows operating systems to modify the execution environment in response to changes in workload behavior and system state. The main challenges of this project included determination of what operating system (OS) algorithms, policies, and parameters should be adapted, when to adapt them, and how to adapt them. We addressed these challenges by using a combination of static analysis and runtime monitoring and adaptation to identify a priori profitable targets of adaptation and effective heuristics that can be used to dynamically trigger adaptation. Dynamic monitoring and adaptation of the OS was provided by either kernel modifications or the use of KernInst and Kperfmon [Wm04]. Since Linux, an open source OS, was our target OS, patches submitted by kernel developers and researchers often facilitated kernel modifications. KernInst operates on unmodified commodity operating systems, i.e., Solaris and Linux; it is fine-grained, thus, there were few constraints on how the underlying OS can be modified. Dynamically adaptive functionality of operating systems, both in terms of policies and parameters, is intended to deliver the maximum attainable performance of a computational environment and meet, as best as possible, the needs of high-performance applications running on extreme scale systems, while meeting system constraints. DAiSES research endeavored to reach this goal by developing methodologies for dynamic adaptation of OS parameters and policies to manage stateful and stateless resources [Te06] and pursuing the following two objectives: (1) Development of mechanisms to dynamically sense, analyze, and adjust common performance metrics, fluctuating workload situations, and overall system environment conditions. (2) Demonstration, via Linux prototypes and experiments, of dynamic self-tuning and self-provisioning in HPC environments. From a high level, the DAiSES methodology, depicted in Figure 1, includes characterization of application resource usage patterns, identification of candidate (profitable) adaptation targets, determination of feasible adaptation ranges, definition of heuristics to trigger adaptation, design and implementation of OS monitoring, triggering, and adaptation code, and quantification of performance gains.

  15. Final Report, DOE grant DE-FG02-99ER45780, "Indirect Excitons in Coupled Quantum Wells"

    SciTech Connect (OSTI)

    Snoke, david W.

    2014-07-21

    The is the final technical report for this project, which was funded by the DOE from 1999 to 2012. The project focused on experimental studies of spatially indirect excitons in coupled quantum wells, with the aim of understanding the quantum physics of these particles, including such effects as pattern formation due to electron-hole charge separation, the Mott plasma-insulator transition, luminescence up-conversion through field-assisted tunneling, luminescence line shifts due to many-body renormalization and magnetic field effects on tunneling, and proposed effects such as Bose-Einstein condensation of indirect excitons and phase separation of bright and dark indirect excitons. Significant results are summarized here and the relation to other work is discussed.

  16. Reconstruction of the constituent distribution and trends in the Antarctic polar vortex from ER-2 flight observations

    SciTech Connect (OSTI)

    Schoeberl, M.R.; Lait, L.R. ); Newman, P.A.; Martin, R.L. ); Proffitt, M.H. ); Hartmann, D.L. ); Loewenstein, M.; Podolske, J.; Strahan, S.E.; Chan, K.R. ); Anderson, J. ); Gary, B. )

    1989-11-30

    Ozone, chlorine monoxide, and nitrous oxide concentrations have been measured in the south polar region. These measurements have been analyzed using conservative coordinate transformations to potential temperature-N{sub 2}O and potential temperature-potential vorticity space. The latter transformation is equivalent to interpreting trace species observations within the modified Lagrangian mean (MLM) coordinate system. The analysis shows that the MLM transformed ozone concentration decreases at about 0.06 ppmv (parts per million by volume) per day between 20 and 16 km altitude inside the polar vortex during the mid-August to mid-September period. These ozone changes must be chemical in origin; they are also collocated with the region of high CIO. Outside the CPR (chemically perturbed region) at the highest aircraft altitudes, ozone systematically increases, suggesting a diabatic cooling of the order of 0.3-0.6 K/d. Within the CPR the cooling rate appears to be less than 0.2 K/d. The MLM analysis technique creates a picture of the general chemical structure of the Austral polar vortex which shows that air deep within the chemically perturbed region has subsided substantially in relation to the air outside. However, there is also a tongue of high ozone air which extends from mid-latitudes downward along the stratospheric jet at 65{degree}W and 60{degree}S. An examination of the last three flight days, September 20-22, 1987, shows that during this period the polar vortex shifts systematically equatorward along the Antarctic Peninsula. Apparent changes in the constituents measured over this period result from sampling air progressively further into the vortex.

  17. Final Technical Report on STTR Project DE-FG02-04ER86191 Hydrogen Cryostat for Muon Beam Cooling

    SciTech Connect (OSTI)

    Johnson, Rolland P.

    2008-05-07

    The project was to develop cryostat designs that could be used for muon beam cooling channels where hydrogen would circulate through refrigerators and the beam-cooling channel to simultaneously refrigerate 1) high-temperature-superconductor (HTS) magnet coils, 2) cold copper RF cavities, and 3) the hydrogen that is heated by the muon beam. In an application where a large amount of hydrogen is naturally present because it is the optimum ionization cooling material, it was reasonable to explore its use with HTS magnets and cold, but not superconducting, RF cavities. In this project we developed computer programs for simulations and analysis and conducted experimental programs to examine the parameters and technological limitations of the materials and designs of Helical Cooling Channel (HCC) components (magnet conductor, RF cavities, absorber windows, heat transport, energy absorber, and refrigerant).The project showed that although a hydrogen cryostat is not the optimum solution for muon ionization cooling channels, the studies of the cooling channel components that define the cryostat requirements led to fundamental advances. In particular, two new lines of promising development were opened up, regarding very high field HTS magnets and the HS concept, that have led to new proposals and funded projects.

  18. Materials Data on Er4In(NiGe2)2 (SG:12) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. Final Project Report for Grant DE-FG03-00ER54581 Selective Control of Chemical Reactions With Plasmas

    SciTech Connect (OSTI)

    Anthony Muscat

    2004-01-28

    OAK-B135 This research work focused on control of the reactive species inside a plasma through measurement and manipulation of the electron energy distribution function (EEDF) and on understanding the surface reaction mechanisms on the substrate exposed to a combination of ion and atom beam sources to simulate a real plasma. A GEC chamber (Gaseous Electronic Conference Reference Cell)8 with a mass spectrometer and a Langmuir probe (LP) system were used for this research. It was found that H2 and N2 additives to an Ar plasma could effectively change the EEDF and the average electron temperature (Te). This finding provides the possibility to selectively control reaction rates in the plasma to control etching selectivity on a surface. This concept was demonstrated in Ar/N2/H2 and Ar/CH4 /H2 systems.

  20. Scientific/Technical Report Science Literacy Project Award number-DE-FG02-06ER64286

    SciTech Connect (OSTI)

    Nasseh, Bizhan

    2011-02-28

    Ball State University (BSU) was the recipient of a U.S. Department of Energy award to develop educational games teaching science and math. The Science Media Program will merge Ball State Universitys nationally recognized capabilities in education, technology, and communication to develop new, interactive, game-based media for the teaching and learning of science and scientific principles for K-12 students. BSU established a team of educators, researchers, scientists, animators, designers, technology specialists, and hired a professional media developer company (Outside Source Design) from Indianapolis. After six months discussions and assessments the project team selected the following 8 games in Math, Physics, Chemistry, and Biology, 2 from each discipline. The assembled teams were innovative and unique. This new model of development and production included a process that integrated all needed knowledge and expertise for the development of high quality science and math games for K-12 students. This new model has potential to be used by others for the development of the educational games. The uniqueness of the model is to integrate domain experts knowledge with researchers/quality control group, and combine a professional development team from the game development company with the academic game development team from Computer Science and Art departments at Ball State University. The developed games went through feasibility tests with selected students for improvement before use in the research activities.

  1. Final Report for EPSCoR Implementation Award DE-FG02-08ER46528 to University of Tennessee

    SciTech Connect (OSTI)

    Egami, Takeshi

    2015-12-15

    With the completion of the Spallation Neutron Source (SNS) and upgrading of the High-Flux Isotope Reactor (HFIR) at the Oak Ridge National Laboratory (ORNL) the state of Tennessee now leads the world in the capability of neutron scattering research. This project aimed at directing the great impact of these facilities to researchers in the EPSCoR states, Tennessee in particular, by creating a research collaboration network around these facilities. The plan consisted of two parts: (1) Direct effort to increase the user base through the travel fellowship for graduate students and faculty from the EPSCoR states to use the neutron facilities at the ORNL, and through workshops and schools on the application of neutron scattering, and (2) Research collaboration among the core participants from UTK, ORNL and other states. The EPSCoR Travel Fellowship Program has supported over 300 distinct and 600 cumulative neutron facility users and over 250 workshop participants, with the total of nearly 600 distinct recipients. This program has been highly popular particularly among young faculty members who often have difficulty in raising travel funds, and enabled participation of young graduate students to neutron research. This program has been the foundation of this project. We supported several educational workshops, organized one (“neutrons for novice”) by ourselves each year, targeting non-users of neutron scattering. These efforts significantly contributed to expand the neutron user base among the EPSCoR states. The core research targeted condensed matter physics and soft matter sciences. The core research groups participating in this project include not only researchers from Tennessee but those from Kansas, South Carolina, Puerto Rico and Louisiana, making this project a national, rather than regional, enterprise. Collaborations that were seeded by this project have grown into two major projects, one in materials science (irradiation effects on high-entropy alloys) and the other in soft matter sciences (bio-membranes). Through this project we promoted the use of neutron scattering, particularly in biological and life sciences and in energy sciences, and facilitated the DOE investment in this field to impact wide fields of science and engineering. This project was administered through the Joint Institute for Neutron Sciences (JINS) of the University of Tennessee (UT) and ORNL. JINS is jointly supported by both UT and ORNL, and participate in organizing workshops and schools to promote the use of neutron scattering.

  2. Spallation Neutrons and Pressure ?? SNAP ?? DE-FG02-03ER46085 CLOSE-OUT MAY 2009

    SciTech Connect (OSTI)

    John B Parise

    2009-05-22

    The purpose of the grant was to build a community of scientist and to draw upon their expertise to design and build the world's first dedicated high pressure beamline at a spallation source - the so called Spallation Neutron And Pressure (SNAP) beamline at the Spallation Neutron Source (SNS) at OAk Ridge NAtional LAboratory. . Key to this endeavor was an annual meeting attended by the instrument design team and the executive committee. The discussions at those meeting set an ambitious agenda for beamline design and construction and highlighted key science areas of interest for the community. This report documents in 4 appendices the deliberations at the annual SNAP meetings and the evolution of the beamline optics from concept to construction. The appendices also contain key science opportunities for extreme conditions research.

  3. Amorphous Diamond Flat Panel Displays - Final Report of ER-LTR CRADA project with SI Diamond Technology

    SciTech Connect (OSTI)

    Ager III, Joel W.

    1998-05-08

    The objective of this project was to determine why diamond-based films are unusually efficient electron emitters (field emission cathodes) at room temperature. Efficient cathodes based on diamond are being developed by SI Diamond Technology (SIDT) as components for bright, sunlight-readable, flat panel displays. When the project started, it was known that only a small fraction (<1%) of the cathode area is active in electron emission and that the emission sites themselves are sub-micron in size. The critical challenge of this project was to develop new microcharacterization methods capable of examining known emission sites. The research team used a combination of cathode emission imaging (developed at SIDT), micro-Raman spectroscopy (LBNL), and electron microscopy and spectroscopy (National Center for Electron Microscopy, LBNL) to examine the properties of known emission sites. The most significant accomplishment of the project was the development at LBNL of a very high resolution scanning probe that, for the first time, measured simultaneously the topography and electrical characteristics of single emission sites. The increased understanding of the emission mechanism helped SIDT to develop a new cathode material,''nano-diamond,'' which they have incorporated into their Field Emission Picture Element (FEPix) product. SIDT is developing large-format flat panel displays based on these picture elements that will be brighter and more efficient than existing outdoor displays such as Jumbotrons. The energy saving that will be realized if field emission displays are introduced commercially is in line with the energy conservation mission of DOE. The unique characterization tools developed in this project (particularly the new scanning microscopy method) are being used in ongoing BES-funded basic research.

  4. FINAL REPORT DE-FG02-04ER41317 Advanced Computation and Chaotic Dynamics for Beams and Accelerators

    SciTech Connect (OSTI)

    Cary, John R

    2014-09-08

    During the year ending in August 2013, we continued to investigate the potential of photonic crystal (PhC) materials for acceleration purposes. We worked to characterize acceleration ability of simple PhC accelerator structures, as well as to characterize PhC materials to determine whether current fabrication techniques can meet the needs of future accelerating structures. We have also continued to design and optimize PhC accelerator structures, with the ultimate goal of finding a new kind of accelerator structure that could offer significant advantages over current RF acceleration technology. This design and optimization of these requires high performance computation, and we continue to work on methods to make such computation faster and more efficient.

  5. Final Report, DE-FG02-92ER14261, Pore Scale Geometric and Fluid Distribution Analysis

    SciTech Connect (OSTI)

    W. Brent Lindquist

    2005-01-21

    The elucidation of the relationship between pore scale structure and fluid flow in porous media is a fundamental problem of long standing interest. Incomplete characterization of medium properties continues to be a limiting factor in accurate field scale simulations. The accomplishments of this grant have kept us at the forefront in investigating the applicability of X-ray computed microtomography (XCMT) as a tool for contributing to the understanding of this relationship. Specific accomplishments have been achieved in four areas: - development of numerical algorithms (largely in the field of computational geometry) to provide automated recognition of and measurements on features of interest in the pore space. These algorithms have been embodied in a software package, 3DMA-Rock. - application of these algorithms to extensive studies of the pore space of sandstones. - application of these algorithms to studies of fluid (oil/water) partitioning in the pore space of Berea sandstone and polyethylene models. - technology transfer.

  6. Electrically active Er doping in InAs, In[sub 0.53]Ga[sub 0.47...

    Office of Scientific and Technical Information (OSTI)

    Country of Publication: United States Language: English Subject: solar (photovoltaic), solid state lighting, phonons, thermoelectric, bio-inspired, energy storage (including ...

  7. Final Report for 3-year grant no. DE-FG05-85ER40226. Investigations in Elementary Particle Theory.

    SciTech Connect (OSTI)

    Kephart, Thomas W.; Scherrer, Robert J.; Weiler, Thomas J.

    2014-11-23

    The research interests of our three Co-PIs complement each other very well. Kephart works mainly on models of particle unification in four or higher dimensions, on aspects of gravity such as inflation, black-holes, and the very early Universe, and on applications of knot theory and topology to various physical systems (including gluon dynamics). Scherrer works mainly on aspects of the intermediate-aged Universe, including dark matter and dark energy, and particle physics in the early Universe. Weiler works mainly on neutrino physics, dark matter signatures, and extreme particle-astrophysics in the late Universe, including origins of the highest-energy cosmic-rays and gamma-rays, and the future potential of neutrino astrophysics. Kephart and Weiler have lately devoted some research attention to the LHC and its reach for probing physics beyond the Standard Model. During the 3-year funding period, our grant supported one postdoc (Chiu Man Ho) and partially supported two students, Peter Denton and Lingjun Fu. Chiu Man collaborated with all three of the Co-PIs during the 3-year funding period and published 16 refereed papers. Chiu Man has gone on to a postdoc with Steve Hsu at Michigan State University. Denton and Fu will both receive their PhDs during the 2014-15 academic year. The total number of our papers published in refereed journals by the three co-PIs during the period of this grant (2011-present) is 54. The total number of talks given by the group members during this time period, including seminars, colloquia, and conference presentations, is 47. Some details of the accomplishments of our DOE funded researchers during the grant period include Weiler being named a Simons Fellow in 2013. He presented an invited TEDx talk in 2012. His paper on closed timelike curves (2013) garnered a great deal of national publicity. Scherrers paper on the little rip (2011) fostered a new area of cosmological research, and the name little rip has now entered the lexicon of cosmology. His paper on anapole dark matter (2013) generated a great deal of interest in the community. Kephart was one of the co-organizers for a 5-month program on applications of topology to physics and biology, and a Visiting Fellow at the Isaac Newton Institute in Cambridge, UK for the fall semester of 2012. His research there resulted in an extended publication on topologically stabilized closed QCD flux tube states.

  8. Santa Cruz Institute for Particle Physics (SCIPP) DOE Grant DE-FG02-04ER41285 Final Report

    SciTech Connect (OSTI)

    Ritz, Steven

    2013-07-26

    Research in High-Energy particle physics and astrophysics is done in the setting of an Organized Research Unit, the Santa Cruz Institute for Particle Physic.

  9. Department of Energy DE-FG02-05ER25686 Early Career Principal Investigator Award Final Report

    SciTech Connect (OSTI)

    Derek, Chiou

    2013-05-11

    This project is researching FAST, a methodology to build very fast, cycleaccurate full system computer simulators and building the first set of such simulators and the first set of tools to help construct those simulators. The methodology relies on a functional model that is a fast, full-system but not cycle-accurate simulator coupled with a timing model that is models the micro-architectural structure and arbitration of a computer system, but not its functionality. The way FAST simulators differ from other simulators partitioned in the same way is that the interface between the functional and timing model is optimized to minimize the need for round-trip communication. The optimized communication enables FAST timing models to be implemented in an FPGA and the functional model in software while still achieving extremely high performance. Our general strategy is to design a methodology and then prototype an example using that methodology to ensure the methodology is sound before committing to it. This strategy ensures that the selected methodology has been tested and provides an early example of the output of that methodology.

  10. Materials Data on Sr2ErCu3(PbO4)2 (SG:123) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2015-02-19

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. Report No.: DOE/ DE-FG02-09ER64747-3 DOE Project No.: DE-FG02...

    Office of Scientific and Technical Information (OSTI)

    ... To our knowledge, none of the extant throat finding algorithms account for the possibility ... Figure 3.1 depicts a conceptualization of the two methods for informing the pore network ...

  12. FBP-ER-RIFS-BG-PLN-0036 Rev. 6 1 DOE/PPPO/03-0383&D4

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... (as measured by ex cess lifetim e cancer risk ELCR to humans) or from other ... Excess Lifetime Cancer Risk (ELCR) - ELCR considers the cumulative probability of humans ...

  13. Final Technical Report DE-FG02-99ER14933 Inversion of multicomponent seismic data and rock physics interpretation

    SciTech Connect (OSTI)

    Mavko, G.

    2006-03-15

    An important accomplishment was to understand the seismic velocity anisotropy resulting from the combined roles of depositional stratification and stress in unconsolidated sands. The report presents an experimental study of velocity anisotropy in unconsolidated sands at measured compressive stresses up to 40 bars, which correspond to the first hundred meters of the subsurface. Two types of velocity anisotropy are considered, that due to intrinsic textural anisotropy, and that due to stress anisotropy. We found that sand samples display a bi-linear dependence of velocity anisotropy with stress anisotropy. There exists a transition stress beyond which the stress-induced anisotropy outweighs the intrinsic anisotropy for three different sands.

  14. ERS 14.3 Underground and Above Ground Diesel Fuel Storage Tanks FPS 12.1, 1/9/01

    Broader source: Energy.gov [DOE]

     The objective of this surveillance is to verify underground and above ground diesel storage tanks are maintained, monitored, configured and marked as required.  These surveillance activities...

  15. WH ITNEY CAN YON-CART ER CR K YELLOW CR EEK_WY_D PIN EVIEW AN

    U.S. Energy Information Administration (EIA) Indexed Site

    BOE Reserve Class No 2001 reserves 0.1 - 10 MBOE 10.1 - 100 MBOE 100.1 - 1,000 MBOE 1,000.1 - 10,000 MBOE 10,000.1 - 100,000 MBOE > 100,000 MBOE Basin Outline WY UT ID INDEX MAP ...

  16. WH ITNEY CAN YON-CART ER CR K YELLOW CR EEK_WY_D PIN EVIEW AN

    U.S. Energy Information Administration (EIA) Indexed Site

    Gas Reserve Class No 2001 gas reserves 0.1 - 10 MMCF 10.1 - 100 MMCF 100.1 - 1,000 MMCF 1,000.1 - 10,000 MMCF 10,000.1 - 100,000 MMCF > 100,000 MMCF Basin Outline WY UT ID INDEX ...

  17. ERS 14.3 Underground and Above Ground Diesel Fuel Storage Tanks FPS 12.1, 1/9/01

    Broader source: Energy.gov [DOE]

    The objective of this surveillance is to verify underground and above ground diesel storage tanks are maintained, monitored, configured and marked as required.  These surveillance activities...

  18. WH ITNEY CAN YON-CART ER CR K YELLOW CR EEK_WY_D PIN EVIEW AN

    U.S. Energy Information Administration (EIA) Indexed Site

    Liquids Reserve Class No 2001 liquids reserves 0.1 - 10 Mbbl 10.1 - 100 Mbbl 100.1 - 1,000 Mbbl 1,000.1 - 10,000 Mbbl 10,000.1 - 100,000 Mbbl Basin Outline WY UT ID INDEX MAP 2001 ...

  19. Final Technical Report for Grant No. DE-FG02-08ER15926: Assuring the Integrity of Research Data

    SciTech Connect (OSTI)

    Thomas Arrison

    2010-02-05

    This letter serves as the final technical report for the National Academy of Sciences project on Ensuring the Integrity of Research Data.

  20. FBP-ER-RIFS-BG-PLN-0036 Rev. 6 1 DOE/PPPO/03-0383&D4

    Office of Environmental Management (EM)

    BG-PLN-0036 Rev. 6 1 DOE/PPPO/03-0383&D4 Aerial photo of the Portsmouth Gaseous Diffusion Plant, showing the approximate 1,000-acre industrialized area within Perimeter Road PUBLIC COMMENT PERIOD NOVEMBER 12, 2014 TO JANUARY 10, 2015 HOW YOU CAN PARTICIPATE Read this Proposed Plan and review related documents in the Administrative Record. Comment on this Proposed Plan by mail, email, phone, or fax to: Ms. Kristi Wiehle Department of Energy P.O. Box 370 Piketon, Ohio 45661 Email:

  1. Final technical report for: Insertional Mutagenesis of Brachypodium distachyon DE-AI02-07ER64452

    SciTech Connect (OSTI)

    John, Vogel P.

    2015-10-29

    Several bioenergy grasses are poised to become a major source of energy in the United States. Despite their increasing importance, we know little about the basic biology underlying the traits that control the utility of grasses as energy crops. Better knowledge of grass biology (e.g. identification of the genes that control cell wall composition, plant architecture, cell size, cell division, reproduction, nutrient uptake, carbon flux, etc.) could be used to design rational strategies for crop improvement and shorten the time required to domesticate these species. The use of an appropriate model system is an efficient way to gain this knowledge. Brachypodium distachyon is a small annual grass with all the attributes needed to be a modern model organism including simple growth requirements, fast generation time, small stature, small genome size and self-fertility. These attributes led to the recommendation in the DOEs Breaking the Biological Barriers to Cellulosic Ethanol: A Joint Research Agenda report to propose developing and using B. distachyon as a model for energy crops to accelerate their domestication. Strategic investments (e.g. genome sequencing) in B. distachyon by the DOE are now bearing fruit and B. distachyon is being used as a model grass by hundreds of laboratories worldwide. Sequence indexed insertional mutants are an extremely powerful tool for both forward and reverse genetics. They allow researchers to order mutants in any gene tagged in the collection by simply emailing a request. The goal of this project was to create a collection of sequence indexed insertional mutants (T-DNA lines) for the model grass Brachypodium distachyon in order to facilitate research by the scientific community. During the course of this grant we created a collection of 23,649 B. distachyon T-DNA lines and identified 26,112 unique insertion sites. The collection can be queried through the project website (http://jgi.doe.gov/our-science/science-programs/plant-genomics/brachypodium/brachypodium-t-dna-collection/) and through the Phytozome genome browser (http://phytozome.jgi.doe.gov/pz/portal.html). The collection has been heavily utilized by the research community and, as of October 23, 2015, 223 orders for 12,069 seeds packets have been filled. In addition to creating this resource, we also optimized methods for transformation and sequencing DNA flanking insertion sites.

  2. Delayed myelosuppression with acute exposure to hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and environmental degradation product hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX) in rats

    SciTech Connect (OSTI)

    Jaligama, Sridhar; Kale, Vijay M.; Wilbanks, Mitchell S.; Perkins, Edward J.; Meyer, Sharon A.

    2013-02-01

    Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a widely used munitions compound, and hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX), its N-nitroso product of anaerobic microbial nitroreduction, are contaminants of military sites. Previous studies have shown MNX to be the most acutely toxic among the nitroreduced degradation products of RDX and to cause mild anemia at high dose. The present study compares hematotoxicity with acute oral exposure to MNX with parent RDX. Both RDX and MNX caused a modest decrease in blood hemoglobin and ? 50% loss of granulocytes (NOAELs = 47 mg/kg) in female SpragueDawley rats observed 14 days post-exposure. We explored the possibility that blood cell loss observed after 14 days was delayed in onset because of toxicity to bone marrow (BM) progenitors. RDX and MNX decreased granulocyte/macrophage-colony forming cells (GM-CFCs) at 14, but not 7, days (NOAELs = 24 mg/kg). The earliest observed time at which MNX decreased GM-CFCs was 10 days post-exposure. RDX and MNX likewise decreased BM burst-forming units-erythroid (BFU-Es) at 14, but not 7, days. Granulocyteerythrocytemonocytemegakaryocyte (GEMM)-CFCs were unaffected by RDX and MNX at 7 days suggesting precursor depletion did not account for GM-CFC and BFU-E loss. MNX added to the culture media was without effect on GM-CFC formation indicating no direct inhibition. Flow cytometry showed no differential loss of BM multilineage progenitors (Thy1.1{sup +}) or erythroid (CD71{sup +}) precursors with MNX suggesting myeloid and erythroid lineages were comparably affected. Collectively, these data indicate that acute exposure to both RDX and MNX caused delayed suppression of myelo- and erythropoiesis with subsequent decrease of peripheral granulocytes and erythrocytes. Highlights: ? Acute oral exposure to munitions RDX causes myelosuppression. ? Environmental degradation product MNX is comparable in effect. ? RDX and MNX are cytotoxic to both myeloid and erythroid progenitor cells. ? Myelosuppression is delayed in onset by > 7 days after single exposure.

  3. The endoplasmic reticulum is a target organelle for trivalent dimethylarsinic acid (DMA{sup III})-induced cytotoxicity

    SciTech Connect (OSTI)

    Naranmandura, Hua, E-mail: narenman@zju.edu.cn [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China)] [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China); Xu, Shi [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China)] [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China); Koike, Shota [Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan)] [Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan); Pan, Li Qiang [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China)] [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China); Chen, Bin [Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030 (China)] [Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030 (China); Wang, Yan Wei; Rehman, Kanwal; Wu, Bin [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China)] [Department of Pharmacology, Toxicology, and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058 (China); Chen, Zhe [Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou (China)] [Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou (China); Suzuki, Noriyuki, E-mail: n-suzuki@p.chiba-u.ac.jp [Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan)] [Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675 (Japan)

    2012-05-01

    The purpose of present study was to characterize the endoplasmic reticulum stress and generation of ROS in rat liver RLC-16 cells by exposing to trivalent dimethylarsinous acid (DMA{sup III}) and compared with that of trivalent arsenite (iAs{sup III}) and monomethylarsonous acid (MMA{sup III}). Protein kinase-like endoplasmic reticulum kinase (PERK) phosphorylation was significantly induced in cells exposed to DMA{sup III}, while there was no change in phosphorylated PERK (P-PERK) detected in cells after exposure to iAs{sup III} or MMA{sup III}. The generation of reactive oxygen species (ROS) after DMA{sup III} exposure was found to take place specifically in the endoplasmic reticulum (ER), while previous reports showed that ROS was generated in mitochondria following exposure to MMA{sup III}. Meanwhile, cycloheximide (CHX) which is an inhibitor of protein biosynthesis strongly inhibited the DMA{sup III}-induced intracellular ROS generation in the ER and the phosphorylation of PERK, suggesting the induction of ER stress probably occurs through the inhibition of the protein folding process. Activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP) mRNA were induced by all three arsenic species, however, evidence suggested that they might be induced by different pathways in the case of iAs{sup III} and MMA{sup III}. In addition, ER resident molecular chaperone glucose-regulated protein78 (GRP78) was not affected by trivalent arsenicals, while it was induced in positive control only at high concentration (Thapsigargin;Tg), suggesting the GRP78 is less sensitive to low levels of ER stress. In summary, our findings demonstrate that the endoplasmic reticulum is a target organelle for DMA{sup III}-induced cytotoxicity. Highlights: ?ER is a target organelle for trivalent DMA{sup III}-induced cytotoxicity. ?Generation of ROS in ER can be induced specially by trivalent DMA{sup III}. ?ER-stress and generation of ROS are caused by the increase in unfolded proteins.

  4. BIG RU N INDIANA LAKESHORE RUN E LUMBER CIT Y WARSAW JOHNST

    Gasoline and Diesel Fuel Update (EIA)

    RU N INDIANA LAKESHORE RUN E LUMBER CIT Y WARSAW JOHNST OWN BU RNSIDE MILLSTONE FROSTBUR G JUN EAU PLU MVILLE CHERRY HILL KAN E BOSWELL MAR ION CENT ER CREEKSIDE SALTSBUR G POINT N BLAIR SVILL E COU NCIL RU N SIGEL LEWISVILLE BEAR C REEK AR MBRUST OHIOPYLE HALLT ON BR OOKVILLE MAR KTON NOL O RAT HMEL COR SICA MAR CHAND SMIC KSBU RG HOWE APOLLO SEVEN SPRIN GS YAT ESBORO MCNEES LUCIND A GEORGE PIN EY LEEPER TIMBLIN WILL ET FERGUSON CLIMAX PANIC DAVY HILL TIDIOUT E GRAMPIAN SLIGO ROC KVI LLE

  5. Final Report for grant DE-FG02-06ER54888, "Simulation of Beam-Electron Cloud Interactions in Circular Accelerators Using Plasma Models"

    SciTech Connect (OSTI)

    Decyk, Viktor K.

    2012-11-27

    The primary goal of this collaborative proposal was to modify the code QuickPIC and apply it to study the long-time stability of beam propagation in low density electron clouds present in circular accelerators. The UCLA contribution to this collaborative proposal was in supporting the development of the pipelining scheme for the QuickPIC code, which extended the parallel scaling of this code by two orders of magnitude.

  6. Final Report for DOE grant no. DE-FG02-04ER63883: Can soil genomics predict the impact of precipitation on nitrous oxide flux from soil

    SciTech Connect (OSTI)

    Egbert Schwartz

    2008-12-15

    Nitrous oxide is a potent greenhouse gas that is released by microorganisms in soil. However, the production of nitrous oxide in soil is highly variable and difficult to predict. Future climate change may have large impacts on nitrous oxide release through alteration of precipitation patterns. We analyzed DNA extracted from soil in order to uncover relationships between microbial processes, abundance of particular DNA sequences and net nitrous oxide fluxes from soil. Denitrification, a microbial process in which nitrate is used as an electron acceptor, correlated with nitrous oxide flux from soil. The abundance of ammonia oxidizing archaea correlated positively, but weakly, with nitrous oxide production in soil. The abundance of bacterial genes in soil was negatively correlated with gross nitrogen mineralization rates and nitrous oxide release from soil. We suggest that the most important control over nitrous oxide production in soil is the growth and death of microorganisms. When organisms are growing nitrogen is incorporated into their biomass and nitrous oxide flux is low. In contrast, when microorganisms die, due to predation or infection by viruses, inorganic nitrogen is released into the soil resulting in nitrous oxide release. Higher rates of precipitation increase access to microorganisms by predators or viruses through filling large soil pores with water and therefore can lead to large releases of nitrous oxide from soil. We developed a new technique, stable isotope probing with 18O-water, to study growth and mortality of microorganisms in soil.

  7. Final Report on Activities Supported by Department of Energy Grant No. DE-FG02-02ER63397, 2002-August 31, 2005

    SciTech Connect (OSTI)

    Madelaine Marquez; Neil Stillings

    2005-09-07

    The grant supported four projects that involved professional development for teachers and enrichment programs for students from under-funded and under-served school districts. The projects involved long-term partnerships between Hampshire College and the districts. All projects were concerned with the effective implementation of inquiry-based science learning and its alignment with state and national curriculum and assessment standards. One project, The Collaboration for Excellence in Science Education (CESE), was designed to support research on the development of concepts in the physical sciences, specifically energy and waves. Extensive data from student interviews and written essays supported the neo-Piagetian hierarchical complexity theory of this area of conceptual development. New assessment techniques that can be used by teachers were also developed. The final report includes a full presentation of the methods and results of the research.

  8. Final Technical Report on STTR Project DE-FG02-06ER86282 Development and Demonstration of 6-Dimensional Muon Beam Cooling

    SciTech Connect (OSTI)

    Muons, Inc.

    2011-05-24

    The overarching purpose of this project was to prepare a proposal for an experiment to demonstrate 6-dimensional muon beam cooling. The technical objectives were all steps in preparing the proposal, which was successfully presented to the Fermilab Accelerator Advisory Committee in February 2009. All primary goals of this project have been met.

  9. Final Scientific/Technical Report for DE-FG02-07ER64500 Study of Lignocellulosic Material Degradation with CARS Microscopy

    SciTech Connect (OSTI)

    Xie, Xiaoliang Sunney; Ding, Shi-You

    2013-09-30

    The program of research undertaken by our Harvard group, in collaboration with Dr. Ding at the National Renewable Energy Laboratory (NREL) in Golden, CO, seeks to introduce, validate and apply a new analytical technique to study the conversion of lignocellulosic biomass into ethanol. This conversion process has been the subject of intense interest over the past few years because of its potential to provide a clean, renewable source of energy to meet increasing global demand. During the funding period, we have clearly demonstrated visualization of lignin and cellulose using intrinsic vibrational contrast with simulated Raman scattering (SRS) microscopy, developed at Harvard. Our approach offers high spatial resolution and time resolution that is sufficient to capture the kinetics of a pre?treatment process. This is reflected by the publications listed below, as well as the use of SRS microscopy at NREL as a routine analysis tool for research on lignocellulosic biomass. In our original proposal, we envisioned moving to near?field CARS imaging in order to perform chemical mapping at the nanoscale. However, given the dramatic progress made by our group in SRS imaging, we concentrated our efforts on using multi?component SRS (lignin, cellulose, lipid, water, protein, deuterated metabolites, etc.) to quantitatively understand the spatially dispersed kinetics in a variety of plant samples under a variety of conditions. In addition, we built a next generation laser system based on fiber laser technology that allowed rugged and portable instrumentation for SRS microscopy. We also pursued new imaging approaches to improve the acquisition speed of SRS imaging of lignocellulose without sacrificing signal?to?noise ratio. This allowed us to image larger volumes of tissue with higher time resolution to get a more comprehensive picture of the heterogeneity of this chemical process from the submicron up to the centimeter scale.

  10. FINAL REPORT: FG02-01ER-45906 - A novel class of artificially modulated magnetic multilayers based on magnetic shape memory alloys

    SciTech Connect (OSTI)

    Chopra, H. D.

    2005-06-20

    The temperature dependent micromagnetic behavior, structural phase transitions, magnetic transition, and the dynamics of phase transitions have been investigated using magnetic shape memory alloys. Results provide a novel concept of ?polymagnets?. In thin film form the number of martensite variants is greatly reduced. A new technique to study dynamics of magnetic phase transitions has been developed applicable to bulk, thin films or multilayers. Transition pathways in magnetic shape memory alloys are as follows: Structural transitions followed by magnetic transition on cooling, and magnetic transition followed by structural transition on heating. The anisotropy of exchange spring mulilayers is greatly sensitive to substrate constraints, and shows a marked rotational hysteresis at lower temperatures. Results also show that a large reduction in coercivity of multilayers is a direct result of broadening of domain wall width by interlayer magnetostatic interactions.

  11. Final Project Report: DOE Award FG02?04ER25606 Overlay Transit Networking for Scalable, High Performance Data Communication across Heterogeneous Infrastructure

    SciTech Connect (OSTI)

    Micah Beck; Terry Moore

    2007-08-31

    As the flood of data associated with leading edge computational science continues to escalate, the challenge of supporting the distributed collaborations that are now characteristic of it becomes increasingly daunting. The chief obstacles to progress on this front lie less in the synchronous elements of collaboration, which have been reasonably well addressed by new global high performance networks, than in the asynchronous elements, where appropriate shared storage infrastructure seems to be lacking. The recent report from the Department of Energy on the emerging 'data management challenge' captures the multidimensional nature of this problem succinctly: Data inevitably needs to be buffered, for periods ranging from seconds to weeks, in order to be controlled as it moves through the distributed and collaborative research process. To meet the diverse and changing set of application needs that different research communities have, large amounts of non-archival storage are required for transitory buffering, and it needs to be widely dispersed, easily available, and configured to maximize flexibility of use. In today's grid fabric, however, massive storage is mostly concentrated in data centers, available only to those with user accounts and membership in the appropriate virtual organizations, allocated as if its usage were non-transitory, and encapsulated behind legacy interfaces that inhibit the flexibility of use and scheduling. This situation severely restricts the ability of application communities to access and schedule usable storage where and when they need to in order to make their workflow more productive. (p.69f) One possible strategy to deal with this problem lies in creating a storage infrastructure that can be universally shared because it provides only the most generic of asynchronous services. Different user communities then define higher level services as necessary to meet their needs. One model of such a service is a Storage Network, analogous to those used within computation centers, but designed to operate on a global scale. Building on a basic storage service that is as primitive as possible, such a Global Storage Network would define a framework within which higher level services can be created. If this framework enabled a variety of more specialized middleware and supported a wide array of applications, then interoperability and collaboration could occur based on that common framework. The research in Logistical Networking (LN) carried out under the DOE's SciDAC program tested the value of this approach within the context of several SciDAC application communities. Below we briefly describe the basic design of the LN storage network and some of the results that the Logistical Networking community has achieved.

  12. Harness: Heterogeneous Adaptable Reconfigurable Networked Systems -- U.S. Department of Energy Grant DE-FG02-99ER25379 Final Project Report

    SciTech Connect (OSTI)

    Vaidy Sunderam

    2003-07-02

    Issues in reconfigurability and adaptability in heterogeneous distributed systems for high-performance computing are the focus of the work funded by this grant. Our efforts are part of an ongoing research project in metacomputing and are a follow on to the DOE funded PVM system that has witnessed over a decade of use at numerous institutions worldwide. The current project, termed Harness, investigates novel methodologies and tools for distributed metacomputing, focusing on dynamically reconfigurable software frameworks. During the first phase, we defined the metacomputing architecture embodied in Harness and developed prototype subsystems as proof of concept exercises. Subsequently, we designed and developed a complete software framework manifesting the Harness architecture, and also developed several tools and subsystems that demonstrated the viability and effectiveness of our proposed model for next generation metacomputing. We then used this substrate to emulate multiple programming environments on Harness, and conducted performance evaluation and tuning exercises. The main research results from these efforts include the establishment of software metacomputing systems as viable and cost-effective alternatives to MPPs; the demonstration of dynamic and reconfigurable platforms as effective methods of tailoring parallel computing environments; the development of methodologies to construct plugin modules for component-based distributed systems; contributions to performance modeling and optimization in emulated software environments; and software architectures for multi- and mixed-paradigm parallel distributed computing. Details and specifics on these and other results have been reported in numerous publications, and are manifested in software systems, all of which may be accessed at or via the website http://www.mathcs.emory.edu/harness/

  13. Hampshire College Center for Science Education Final Report on Activities Supported by the Department of Energy Grant No. DE-FG02-06ER64256

    SciTech Connect (OSTI)

    Stillings, Neil, Professor of Psychology; Wenk, Laura, Assistant Professor of Cognition and Education

    2009-12-30

    Hampshire College's Center for Science Education (Center) focuses on teacher professional development, curriculum development, and student enrichment programs. The Center also maintains research programs on teacher change, student learning and instructional effectiveness. The Center's work promotes learning that persists over time and transfers to new situations in and out of school. The projects develop the implications of the increasing agreement among teachers and researchers that effective learning involves active concept mastery and consistent practice with inquiry and critical thinking. The Center's objective is to help strengthen the pipeline of U.S. students pursuing postsecondary study in STEM fields. The Center achieves this by fostering an educational environment in which science is taught as an active, directly experienced endeavor across the K-16 continuum. Too often, young people are dissuaded from pursuing science because they do not see its relevance, instead experiencing it as dry, rote, technical. In contrast, when science is taught as a hands-on, inquiry-driven process, students are encouraged to ask questions grounded in their own curiosity and seek experimental solutions accordingly. In this way, they quickly discover both the profound relevance of science to their daily lives and its accessibility to them. Essentially, they learn to think and act like real scientists. The Centers approach is multi-faceted: it includes direct inquiry-based science instruction to secondary and postsecondary students, educating the next generation of teachers, and providing new educational opportunities for teachers already working in the schools. Funding from the Department of Energy focused on the last population, enabling in-service teachers to explore and experience the pedagogy of inquiry-based science for themselves, and to take it back to their classrooms and students. The Center has demonstrated that the inquiry-based approach to science learning is compatible with existing state curriculum frameworks and produces students who understand and are positively inclined toward science. Funds from this Department of Energy grant supported three projects that involved K-16 science outreach: 1. Teaching Issues and Experiments in Ecology (TIEE). TIEE a peer-reviewed online journal and curriculum resource for postsecondary science teachers. 2. The Collaboration for Excellence in Science Education (CESE). CESE is a partnership with the Amherst, Massachusetts school system to foster the professional development of science teachers, and to perform research on student learning in the sciences and on teacher change. The project draws on Hampshire's long experience with inquiry-oriented and interdisciplinary education, as well as on its unique strengths in cognitive science. The project is run as design research, working with teachers to improve their practices and studying student and/or teacher outcomes. 3. Day in the Lab. Grant funds partially supported the expansion of the ongoing science outreach activities of the School of Natural Science. These activities are focused on local districts with large minority enrollments, including the Amherst, Holyoke and Springfield Public School Districts, and the Pioneer Valley Performing Arts Charter School (PVPA). Each of the three projects supported by the grant met or exceeded its goals. In part, the successes we met were due to continuity and communication among the staff of the programs. At the beginning of the CESE project, a science outreach coordinator was recruited. He worked throughout the grant period along with a senior researcher and the project's curriculum director. Additionally, the director and an undergraduate student conducted research on teacher change. The science outreach coordinator acted as a liaison among Hampshire College, the school districts, and a number of local businesses and agencies, providing organizational support, discussion facilitation, classroom support for teachers, and materials purchase. His presence in the schools kept teachers engaged and

  14. Final Technical Report for Collaborative Research: Regional climate-change projections through next-generation empirical and dynamical models, DE-FG02-07ER64429

    SciTech Connect (OSTI)

    Smyth, Padhraic

    2013-07-22

    This is the final report for a DOE-funded research project describing the outcome of research on non-homogeneous hidden Markov models (NHMMs) and coupled ocean-atmosphere (O-A) intermediate-complexity models (ICMs) to identify the potentially predictable modes of climate variability, and to investigate their impacts on the regional-scale. The main results consist of extensive development of the hidden Markov models for rainfall simulation and downscaling specifically within the non-stationary climate change context together with the development of parallelized software; application of NHMMs to downscaling of rainfall projections over India; identification and analysis of decadal climate signals in data and models; and, studies of climate variability in terms of the dynamics of atmospheric flow regimes.

  15. Final Report for Geometric Analysis for Data Reduction and Structure Discovery DE-FG02-10ER25983, STRIPES award # DE-SC0004096

    SciTech Connect (OSTI)

    Vixie, Kevin R

    2014-11-27

    This is the final report for the project "Geometric Analysis for Data Reduction and Structure Discovery" in which insights and tools from geometric analysis were developed and exploited for their potential to large scale data challenges.

  16. FINAL TECHNICAL REPORT for grant DE-FG02-93ER14353 ????"Carbon-Hydrogen Bond Functionalization Catalyzed by Transition Metal Systems"

    SciTech Connect (OSTI)

    Alan S. Goldman

    2012-05-21

    Alkanes are our most abundant organic resource but are highly resistant to selective chemical transformations. Alkenes (olefins) by contrast are the single most versatile class of molecules for selective transformations, and are intermediates in virtually every petrochemical process as well as a vast range of commodity and fine chemical processes. Over the course of this project we have developed the most efficient catalysts to date for the selective conversion of alkanes to give olefins, and have applied these catalysts to other dehydrogenation reactions. We have also developed some of the first efficient catalysts for carbonylation of alkanes and arenes to give aldehydes. The development of these catalysts has been accompanied by elucidation of the mechanism of their operation and the factors controlling the kinetics and thermodynamics of C-H bond activation and other individual steps of the catalytic cycles. This fundamental understanding will allow the further improvement of these catalysts, as well as the development of the next generation of catalysts for the functionalization of alkanes and other molecules containing C-H bonds.

  17. DOE ER63951-3 Final Report: An Integrated Assessment of Geochemical and Community Structure Determinants of Metal Reduction Rates in Subsurface Sediments

    SciTech Connect (OSTI)

    Susan Pfiffner

    2010-06-28

    The objective of this research was to examine the importance of microbial community structure in influencing uranium reduction rates in subsurface sediments. If the redox state alone is the key to metal reduction, then any organisms that can utilize the oxygen and nitrate in the subsurface can change the geochemical conditions so metal reduction becomes an energetically favored reaction. Thus, community structure would not be critical in determining rates or extent of metal reduction unless community structure influenced the rate of change in redox. Alternatively, some microbes may directly catalyze metal reduction (e.g., specifically reduce U). In this case the composition of the community may be more important and specific types of electron donors may promote the production of communities that are more adept at U reduction. Our results helped determine if the type of electron donor or the preexisting community is important in the bioremediation of metal-contaminated environments subjected to biostimulation. In a series of experiments at the DOE FRC site in Oak Ridge we have consistently shown that all substrates promoted nitrate reduction, while glucose, ethanol, and acetate always promoted U reduction. Methanol only occasionally promoted extensive U reduction which is possibly due to community heterogeneity. There appeared to be limitations imposed on the community related to some substrates (e.g. methanol and pyruvate). Membrane lipid analyses (phospholipids and respiratory quinones) indicated different communities depending on electron donor used. Terminal restriction fragment length polymorphism and clone libraries indicated distinct differences among communities even in treatments that promoted U reduction. Thus, there was enough metabolic diversity to accommodate many different electron donors resulting in the U bioimmobilization.

  18. Final Technical Report for DOE Grant, number DE-FG02-05ER15701; Probing Surface Chemistry Under Catalytic Conditions: Olefin Hydrogenation,Cyclization and Functionalization.

    SciTech Connect (OSTI)

    Neurock, Matthew

    2011-05-26

    The specific goal of this work was to understanding the catalytic reactions pathways for the synthesis of vinyl acetate over Pd, Au and PdAu alloys. A combination of both experimental methods (X-ray and Auger spectroscopies, low-energy ion scattering (LEIS), low-energy electron diffraction (LEED) and theory (Density Functional Theory (DFT) calculations and Monte Carlo methods under various different reactions) were used to track the surface chemistry and the influence of alloying. The surface intermediates involved in the various reactions were characterized using reflection-absorption infrared spectroscopy and LEED to identify the nature of the surface species and temperature-programmed desorption (TPD) to follow the decomposition pathways and measure heats of adsorption. These results along with those from density functional theoretical calculations were used determine the kinetics for elementary steps. The results from this work showed that the reaction proceeds via the Samanos mechanism over Pd surfaces whereby the ethylene directly couples with acetate to form an acetoxyethyl intermediate that subsequently undergoes a beta-hydride elimination to form the vinyl acetate monomer. The presence of Au was found to modify the adsorption energies and surface coverages of important surface intermediates including acetate, ethylidyne and ethylene which ultimately influences the critical C-H activation and coupling steps. By controlling the surface alloy composition or structure one can begin to control the steps that control the rate and even the mechanism.

  19. FINAL REPORT: DOE CONTRACT NUMBER FG0205ER64026 Biological Neutron Scattering: A Collaboration with the Oak Ridge Center for Structural Molecular Biology

    SciTech Connect (OSTI)

    Jill Trewhella

    2011-01-12

    The overarching goal of this project was to promote applications of small-angle scattering in structural molecular biology by providing model examples of cutting edge applications that demonstrate the unique capabilities and potential of the DOE national user facilities at Oak Ridge, especially the newly commissioned BioSANS. The approach taken was three-fold: (1) to engage in high impact collaborative research projects that would benefit from small-angle neutron scattering to both demonstrate the power of the technique while expanding the potential user community; (2) to provide access to scattering facilities established at the University of Utah to as broad a set of researchers as possible to increase the expertise in small-angle scattering generally; and (3) to develop new methods and tools for small-angle scattering. To these ends, three major research collaborations were pursued that resulted in a significant body of published work where neutron scattering and contrast variation played a major role. These major collaborations involved studies of protein complexes involved in (1) bacterial transcription regulation and adaptive response (a DOE/BER priority area); (2) regulation of cardiac muscle; and (3) neuronal disorders. In addition, to broaden the impact of the project, smaller collaborative efforts were supported that used either small-angle X-ray or neutron scattering. Finally, the DOE supported facilities at the University of Utah were made available to researchers on a service basis and a number of independent groups took advantage of this opportunity. In all of this work, there was an emphasis on the training of students and post docs in scattering techniques, and a set of publications (a book chapter, a review, and an encyclopedia article) were produced to guide the non-specialist potential user of scattering techniques in successful applications of the techniques. We also developed a suite of user friendly web-based computational tools currently being accessed world-wide by researchers as an aid in neutron scattering data interpretation. In all, these collaborative projects and resulted in 29 original refereed journal articles published between 2005 and 2010 and engaged groups from at least 14 Universities (10 US, 4 international) and 3 National Laboratories (2 US, 1 international). An important final initiative from this project was to begin a process for international community agreement on a set of standards for the publication of biomolecular small-angle scattering data. This initiative is being championed with the International Union of Crystallography and has engaged a number of Journal Editors and is a very important step in the maturing of this now burgeoning field.

  20. Final Report Grant No. DE-FG02-98ER20307 Lipopolysaccharide Structures and Genes Required for Root Nodule Development August 1, 2004 to July 31, 2008

    SciTech Connect (OSTI)

    Noel, K. Dale

    2008-12-07

    This project dealt with the plant-bacterial symbiosis that gives rise to root nodules on leguminous plants in which the bacteria carry out nitrogen fixation. Nitrogen fixation, like carbon dioxide fixation, is essential for life on planet earth, and this symbiosis is estimated to account for half of all nitrogen fixed on land. Aside from being important for the sustenance of global life, this ability allows legumes to grow without nitrogen fertilizers. Basic studies such as this project are aimed at understanding the symbiosis well enough that eventually it can be engineered into important crop species so that they no longer depend on nitrogen fertilizer for growth. The production and distribution of excessive fertilizer needed for optimal crop yields is responsible for a significant portion of the energy costs in agriculture. The specific aims of this work were to further the understanding of a bacterial factor that is essential for the symbiotic infection process. This factor is a bacterial surface molecule, lipopolysaccharide O antigen. In this project we showed that, not only the presence, but the specific structure of this molecule is crucial for infection. Although the success of bacterial infections in many pathogenic and mutualistic interactions have been shown to depend on intact O antigen, it has been very rare to establish that specific features of the structure are important. One of the features in this case is the presence of one additional methyl group on one sugar in the O antigen. It is very surprising that such a minor change should have an observable effect. This work sets the stage for biochemical studies of possible plant receptors that may be involved. During the course of this grant period, we developed a method of testing the importance of this bacterial component at stages of nodule development beyond the step that is blocked by null mutation. The method works adequately for this purpose and is being improved. It has implications for testing the roles of other important bacterial factors at multiple stages of nodule development. The project also investigated the biosynthesis of this bacterial factor. It has a complex structure and the first accomplishment was the determination of the sequences of genetic regions known to be important. Next the discovered genes were mutated to identify the 26 that are required for its synthesis. In addition, six others were discovered that are believed to change its structure under various environmental conditions. By studying mutants affected in specific genes, genes were associated with each of the predicted steps in the biosynthesis. Current work is testing the predicted biosynthetic model with studies conducted in vitro with bacterial extracts. Overall, the work funded by this grant establishes this system as a model for host-bacterial interactions based on specific polysaccharide structure. All areas that are needed for a comprehensive model have been significantly advanced: the biological function, the structural features that are crucial, the complete set of bacterial genes involved, and a model for the biosynthesis.