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  1. Desert Queen Geothermal Project | Open Energy Information

    Open Energy Info (EERE)

    ,"group":"","inlineLabel":"","visitedicon":"" Hide Map Location Fernley, NV County Churchill County, NV Geothermal Area Desert Queen Geothermal Area Geothermal Region Northwest...

  2. Geothermometry At Desert Queen Area (Garchar & Arehart, 2008...

    Open Energy Info (EERE)

    Desert Queen Area (Garchar & Arehart, 2008) Jump to: navigation, search GEOTHERMAL ENERGYGeothermal Home Exploration Activity: Geothermometry At Desert Queen Area (Garchar &...

  3. Queen City Forging Company Revitalized by Lab Partnership | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Text Version (CG: Rob Mayer - Queen City Forging Company President) Queen City Forging ... THEY WERE STRUGGLING TO REMAIN COMPETITIVE. (Mayer) the company was really in a state of ...

  4. Queen City Forging Company Revitalized by Lab Partnership | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Queen City Forging Company Revitalized by Lab Partnership Queen City Forging Company Revitalized by Lab Partnership Addthis Description This video tells how Oak Ridge National Laboratory, with support from the Office of Energy Efficiency and Renewable Energy (EERE), teamed up with Queen City Forging, the U.S. Forging Industry, and Infrared Heating Technologies to develop a rapid-infrared heating furnace to produce aluminum turbochargers. Text Version (CG: Rob Mayer - Queen City

  5. Queen s University of Belfast | Open Energy Information

    Open Energy Info (EERE)

    s University of Belfast Jump to: navigation, search Name: Queen(tm)s University of Belfast Place: Belfast, Northern Ireland, United Kingdom Zip: BT7 1NN Product: Academic...

  6. King and Queen County, Virginia: Energy Resources | Open Energy...

    Open Energy Info (EERE)

    Hide Map This article is a stub. You can help OpenEI by expanding it. King and Queen County is a county in Virginia. Its FIPS County Code is 097. It is classified...

  7. SOLVING THE SHUGART QUEEN SAND PENASCO UNIT DECLINING PRODUCTION PROBLEM

    SciTech Connect (OSTI)

    Lowell Deckert

    2000-08-25

    The Penasco Shugart Queen Sand Unit located in sections 8, 9, 16 & 17, T18S, 31E Eddy County New Mexico is operated by MNA Enterprises Ltd. Co. Hobbs, NM. The first well in the Unit was drilled in 1939 and since that time the Unit produced 535,000 bbl of oil on primary recovery and 375,000 bbl of oil during secondary recovery operations that commenced in 1973. The Unit secondary to primary ratio is 0.7, but other Queen waterfloods in the area had considerably larger S/P ratios. On June 25 1999 MNA was awarded a grant under the Department of Energy's ''Technology Development with Independents'' program. The grant was used to fund a reservoir study to determine if additional waterflood reserves could be developed. A total of 14 well bores that penetrate the Queen at 3150 ft are within the Unit boundaries. Eleven of these wells produced oil during the past 60 years. Production records were pieced together from various sources including the very early state production records. One very early well had a resistivity log, but nine of the wells had no logs, and four wells had gamma ray-neutron count-rate perforating logs. Fortunately, recent offset deep drilling in the area provided a source of modern logs through the Queen. The logs from these wells were used to analyze the four old gamma ray-neutron logs within the Unit. Additionally the offset well log database was sufficient to construct maps through the unit based on geostatistical interpolation methods. The maps were used to define the input parameters required to simulate the primary and secondary producing history. The history-matched simulator was then used to evaluate four production scenarios. The best scenario produces 51,000 bbl of additional oil over a 10-year period. If the injection rate is held to 300 BWPD the oil rate declines to a constant 15 BOPD after the first year. The projections are reasonable when viewed in the context of the historical performance ({approx}30 BOPD with a {approx}600 BWPD

  8. Aerosol Observing System Greenhouse Gas (AOS GhG) Handbook (Technical...

    Office of Scientific and Technical Information (OSTI)

    Aerosol Observing System Greenhouse Gas (AOS GhG) Handbook Citation Details In-Document Search Title: Aerosol Observing System Greenhouse Gas (AOS GhG) Handbook The Greenhouse Gas ...

  9. CANTON LAKESHORE CANTON E BEST CON NEAUT GIDD INGS EAST N ELLSWORT

    U.S. Energy Information Administration (EIA) Indexed Site

    MCKEESPORT COR AOPOLIS-MOON REDHAW ST. CLAIR SC ROGGSFIELD FRANKLIN -OAK FOREST RIMERSBURG RENNERD AL E GREENVILL E PAT MOS CRABTR EE BLAC K ASH ROYALT ON N BAKERSTOWN QUEEN ROU GH RUN LUCAS BLAC K H ILL CRESTON WAT TSVILLE WADSWORTH -NORT H OAKLAN D HOM EWORT H UNIT Y ESSELBRUN ALAMED A PAR K-CROOKED RU CHERRY GROVE FRENC HTOWN ST EWART RUN MILL C REEK GLENF IELD-MOU NT NEBO HICKORY E HARRISVILLE E LEST ER GRIGGS CORNERS EN GLAN D WEST VIL LE LAKE BAILEY LAKE OAKFORD BR UNSWICK N HOR ACE

  10. CAPTAIN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MCKEESPORT COR AOPOLIS-MOON REDHAW ST. CLAIR SC ROGGSFIELD FRANKLIN -OAK FOREST RIMERSBURG RENNERD AL E GREENVILL E PAT MOS CRABTR EE BLAC K ASH ROYALT ON N BAKERSTOWN QUEEN ROU GH RUN LUCAS BLAC K H ILL CRESTON WAT TSVILLE WADSWORTH -NORT H OAKLAN D HOM EWORT H UNIT Y ESSELBRUN ALAMED A PAR K-CROOKED RU CHERRY GROVE FRENC HTOWN ST EWART RUN MILL C REEK GLENF IELD-MOU NT NEBO HICKORY E HARRISVILLE E LEST ER GRIGGS CORNERS EN GLAN D WEST VIL LE LAKE BAILEY LAKE OAKFORD BR UNSWICK N HOR ACE

  11. Wendy Queen | Center for Gas SeparationsRelevant to Clean Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bae, Youn Jue; Cho, Eun Seon; Qu, Fen; Sun, Daniel T.; Williams, Teresa E.; Urban, Jeffrey J.; and Queen, Wendy L. Transparent Metal-Organic FrameworkPolymer Mixed Matrix ...

  12. DOE - Office of Legacy Management -- Queen City Barrel Co - OH 41

    Office of Legacy Management (LM)

    Queen City Barrel Co - OH 41 FUSRAP Considered Sites Site: QUEEN CITY BARREL CO. (OH.41) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Cincinnati , Ohio OH.41-1 Evaluation Year: 1987 OH.41-1 Site Operations: Cleaned and reconditioned 30- and 55-gallon drums. OH.41-2 OH.41-3 Site Disposition: Eliminated - Based upon limited scope of operations, potential for residual radioactive contamination from MED or AEC operations considered

  13. Oakland Ravine Stormwater Treatment System Project, Borough of Queens, NYC

    SciTech Connect (OSTI)

    Dinkle, R.E.; Moutal, H.P.; Evans, T.M.; Kloman, L.

    1999-07-01

    Compared to other cities, New York City (NYC) is abundantly endowed with parklands and open spaces, many of which can be utilized to treat and dissipate stormwater runoff flows, in conjunction with the preservation, restoration and creation of ecological systems. Such use of available parklands and open spaces has the benefit of decreasing cost for stormwater treatment and conveyance, while at the same time enhancing the natural biological systems. Through the combined efforts of the NYC Department of Environmental Protection (NYCDEP), which is responsible for stormwater control, and the NYC Department of Parks and Recreation (NYCDPR), which is responsible for preserving and restoring the ecological systems of parklands and open spaces, URS Greiner Woodward Clyde (URSGWC) developed a project to provide for the treatment of stormwater and the attenuation of peak stormwater flows through restoration and creation of wetlands within Oakland Ravine (located in the densely populated northeastern section of the Borough of Queens, NYC). The proposed Oakland Ravine Stormwater Treatment System Project was developed in conjunction with the East River Combined Sewer Overflow (CSO) Abatement Project, which is part of the NYC comprehensive program to reduce CSO discharges into receiving waters. Discharges into Alley Creek through Outfall TI-7, an outfall located about one-half mile northeast of the ravine which has been designated for CSO abatement, will be reduced as a result of the project.

  14. A study of the mechanism of laser welding defects in low thermal expansion superalloy GH909

    SciTech Connect (OSTI)

    Yan, Fei; Wang, Chunming, E-mail: yanxiangfei225@163.com; Wang, Yajun; Hu, Xiyuan; Wang, Tianjiao; Li, Jianmin; Li, Guozhu

    2013-04-15

    In this paper, we describe experimental laser welding of low-thermal-expansion superalloy GH909. The main welding defects of GH909 by laser in the weld are liquation cracks and porosities, including hydrogen and carbon monoxide porosity. The forming mechanism of laser welding defects was investigated. This investigation was conducted using an optical microscope, scanning electron microscope, energy diffraction spectrum, X-ray diffractometer and other methodologies. The results demonstrated that porosities appearing in the central weld were related to incomplete removal of oxide film on the surface of the welding samples. The porosities produced by these bubbles were formed as a result of residual hydrogen or oxygenium in the weld. These elements failed to escape from the weld since laser welding has both a rapid welding speed and cooling rate. The emerging crack in the heat affected zone is a liquation crack and extends along the grain boundary as a result of composition segregation. LavesNi{sub 2}Ti phase with low melting point is a harmful phase, and the stress causes grain boundaries to liquefy, migrate and even crack. Removing the oxides on the surface of the samples before welding and carefully controlling technological parameters can reduce welding defects and improve formation of the GH909 alloy weld. - Highlights: ? It is a new process for the forming of GH909 alloy via laser welding. ? The forming mechanism of laser welding defects in GH909 has been studied. ? It may be a means to improve the efficiency of aircraft engine production.

  15. West Pearl Queen CO2 sequestration pilot test and modeling project 2006-2008.

    SciTech Connect (OSTI)

    Engler, Bruce Phillip; Cooper, Scott Patrick; Symons, Neill Phillip; Bartel, Lewis Clark; Byrer, Charles; Elbring, Gregory Jay; McNemar, Andrea; Aldridge, David Franklin; Lorenz, John Clay

    2008-08-01

    The West Pearl Queen is a depleted oil reservoir that has produced approximately 250,000 bbl of oil since 1984. Production had slowed prior to CO{sub 2} injection, but no previous secondary or tertiary recovery methods had been applied. The initial project involved reservoir characterization and field response to injection of CO{sub 2}; the field experiment consisted of injection, soak, and venting. For fifty days (December 20, 2002, to February 11, 2003) 2090 tons of CO{sub 2} were injected into the Shattuck Sandstone Member of the Queen Formation at the West Pearl Queen site. This technical report highlights the test results of the numerous research participants and technical areas from 2006-2008. This work included determination of lateral extents of the permeability units using outcrop observations, core results, and well logs. Pre- and post-injection 3D seismic data were acquired. To aid in interpreting seismic data, we performed numerical simulations of the effects of CO{sub 2} replacement of brine where the reservoir model was based upon correlation lengths established by the permeability studies. These numerical simulations are not intended to replicate field data, but to provide insight of the effects of CO{sub 2}.

  16. Rob Andrews Joshua Pearce, Andrew Pollard Queen's University Department of Mechanical and Materials Engineering

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Snowfall on PV Systems Rob Andrews Joshua Pearce, Andrew Pollard Queen's University Department of Mechanical and Materials Engineering Calama Consulting (www.calamaconsulting.ca) andrewsr@me.queensu.ca 613-530-0323 Presented at the 2013 Sandia PV Performance Modeling Workshop Santa Clara, CA. May 1-2, 2013 Published by Sandia National Laboratories with the Permission of the Author. 13-4-22 Snow on PV ● How do we measure the effects of snow on PV? ● How can we distinguish snowfall effects

  17. Sulimar Queen environmental restoration project closure package Sandia environmental stewardship exemplar.

    SciTech Connect (OSTI)

    Tillman, Jack B.

    2008-09-01

    In March 2008, Sandia National Laboratories (Sandia), in partnership with the Bureau of Land Management, Roswell Field Office, completed its responsibilities to plug and abandon wells and restore the surface conditions for the Sulimar Queens Unit, a 2,500 acre oil field, in Chaves County, Southeast New Mexico. Sandia assumed this liability in an agreement to obtain property to create a field laboratory to perform extensive testing and experimentation on enhanced oil recovery techniques for shallow oil fields. In addition to plugging and abandoning 28 wells, the project included the removal of surface structures and surface reclamation of disturbed lands associated with all plugged and abandoned wells, access roads, and other auxiliary facilities within unit boundaries. A contracting strategy was implemented to mitigate risk and reduce cost. As the unit is an important wildlife habitat for prairie chickens, sand dune lizards, and mule deer, the criteria for the restoration and construction process were designed to protect and enhance the wildlife habitat. Lessons learned from this project include: (1) extreme caution should be exercised when entering agreements that include future liabilities, (2) partnering with the regulator has huge benefits, and (3) working with industry experts, who were familiar with the work, and subcontractors, who provided the network to complete the project cost effectively.

  18. Human metastatic melanoma cell lines express high levels of growth hormone receptor and respond to GH treatment

    SciTech Connect (OSTI)

    Sustarsic, Elahu G.; Department of Biological Sciences, Ohio University, Athens, OH ; Junnila, Riia K.; Kopchick, John J.

    2013-11-08

    Highlights: Most cancer types of the NCI60 have sub-sets of cell lines with high GHR expression. GHR is highly expressed in melanoma cell lines. GHR is elevated in advanced stage IV metastatic tumors vs. stage III. GH treatment of metastatic melanoma cell lines alters growth and cell signaling. -- Abstract: Accumulating evidence implicates the growth hormone receptor (GHR) in carcinogenesis. While multiple studies show evidence for expression of growth hormone (GH) and GHR mRNA in human cancer tissue, there is a lack of quantification and only a few cancer types have been investigated. The National Cancer Institutes NCI60 panel includes 60 cancer cell lines from nine types of human cancer: breast, CNS, colon, leukemia, melanoma, non-small cell lung, ovarian, prostate and renal. We utilized this panel to quantify expression of GHR, GH, prolactin receptor (PRLR) and prolactin (PRL) mRNA with real-time RT qPCR. Both GHR and PRLR show a broad range of expression within and among most cancer types. Strikingly, GHR expression is nearly 50-fold higher in melanoma than in the panel as a whole. Analysis of human metastatic melanoma biopsies confirmed GHR gene expression in melanoma tissue. In these human biopsies, the level of GHR mRNA is elevated in advanced stage IV tumor samples compared to stage III. Due to the novel finding of high GHR in melanoma, we examined the effect of GH treatment on three NCI60 melanoma lines (MDA-MB-435, UACC-62 and SK-MEL-5). GH increased proliferation in two out of three cell lines tested. Further analysis revealed GH-induced activation of STAT5 and mTOR in a cell line dependent manner. In conclusion, we have identified cell lines and cancer types that are ideal to study the role of GH and PRL in cancer, yet have been largely overlooked. Furthermore, we found that human metastatic melanoma tumors express GHR and cell lines possess active GHRs that can modulate multiple signaling pathways and alter cell proliferation. Based on this data

  19. Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Amore, Antonella; Iadonisi, Alfonso; Vincent, Florence; Faraco, Vincenza

    2015-12-21

    In this paper, the recombinant α-l-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleophilic residue. Thus, the mutant E449G of PoAbf was recombinantly expressed in Pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. The design of a suitable set of hydrolysis experiments in the presence or absence of alcoholic arabinosyl acceptors and/or formate salts allowed to unambiguously identify the residue E449 as the nucleophile residue involvedmore » in the retaining mechanism of this GH51 arabinofuranosidase. 1H NMR analysis was applied for the identification of the products and the assignement of their anomeric configuration.« less

  20. Functional and structural diversity in GH62 α-L-arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kaur, Amrit Pal; Nocek, Boguslaw P.; Xu, Xiaohui; Lowden, Michael J.; Leyva, Juan Francisco; Stogios, Peter J.; Cui, Hong; Leo, Rosa Di; Powlowski, Justin; Tsang, Adrian; et al

    2015-05-01

    The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation, including three genes, abf62A, abf62B and abf62C, predicted to encode glycoside hydrolase family 62 (GH62) enzymes. Transcriptome analysis showed that only abf62A and abf62C are actively expressed during growth on diverse substrates including straws from barley, alfalfa, triticale and canola. The abf62A and abf62C genes were expressed in Escherichia coli and the resulting recombinant proteins were characterized. Calcium-free crystal structures of Abf62C in apo and xylotriose bound forms were determined to 1.23 and 1.48 Å resolution respectively. Site-directed mutagenesismore » confirmed Asp55, Asp171 and Glu230 as catalytic triad residues, and revealed the critical role of non-catalytic residues Asp194, Trp229 and Tyr338 in positioning the scissile α-L-arabinofuranoside bond at the catalytic site. Further, the +2R substrate-binding site residues Tyr168 and Asn339, as well as the +2NR residue Tyr226, are involved in accommodating long-chain xylan polymers. Overall, our structural and functional analysis highlights characteristic differences between Abf62A and Abf62C, which represent divergent subgroups in the GH62 family.« less

  1. Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus

    SciTech Connect (OSTI)

    Amore, Antonella; Iadonisi, Alfonso; Vincent, Florence; Faraco, Vincenza

    2015-12-21

    In this paper, the recombinant α-l-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleophilic residue. Thus, the mutant E449G of PoAbf was recombinantly expressed in Pichia pastoris and its recombinant expression level and reactivity were investigated in comparison to the wild-type. The design of a suitable set of hydrolysis experiments in the presence or absence of alcoholic arabinosyl acceptors and/or formate salts allowed to unambiguously identify the residue E449 as the nucleophile residue involved in the retaining mechanism of this GH51 arabinofuranosidase. 1H NMR analysis was applied for the identification of the products and the assignement of their anomeric configuration.

  2. Functional and structural diversity in GH62 ?-L-arabinofuranosidases from the thermophilic fungus Scytalidium thermophilum

    SciTech Connect (OSTI)

    Kaur, Amrit Pal; Nocek, Boguslaw P.; Xu, Xiaohui; Lowden, Michael J.; Leyva, Juan Francisco; Stogios, Peter J.; Cui, Hong; Leo, Rosa Di; Powlowski, Justin; Tsang, Adrian; Savchenko, Alexei

    2015-05-01

    The genome of the thermophilic fungus Scytalidium thermophilum (strain CBS 625.91) harbours a wide range of genes involved in carbohydrate degradation, including three genes, abf62A, abf62B and abf62C, predicted to encode glycoside hydrolase family 62 (GH62) enzymes. Transcriptome analysis showed that only abf62A and abf62C are actively expressed during growth on diverse substrates including straws from barley, alfalfa, triticale and canola. The abf62A and abf62C genes were expressed in Escherichia coli and the resulting recombinant proteins were characterized. Calcium-free crystal structures of Abf62C in apo and xylotriose bound forms were determined to 1.23 and 1.48 resolution respectively. Site-directed mutagenesis confirmed Asp55, Asp171 and Glu230 as catalytic triad residues, and revealed the critical role of non-catalytic residues Asp194, Trp229 and Tyr338 in positioning the scissile ?-L-arabinofuranoside bond at the catalytic site. Further, the +2R substrate-binding site residues Tyr168 and Asn339, as well as the +2NR residue Tyr226, are involved in accommodating long-chain xylan polymers. Overall, our structural and functional analysis highlights characteristic differences between Abf62A and Abf62C, which represent divergent subgroups in the GH62 family.

  3. Depositional and diagenetic controls on porosity permeability and oil production in McFarland/Magutex (Queen) reservoirs, Andrews County, west Texas

    SciTech Connect (OSTI)

    Holtz, M.H. )

    1991-03-01

    The McFarland/Magutex Queen reservoir complex lies along the northeastern edge of the Central basin platform in the west Texas Permian basin and produces oil from the Permian Queen Formation. Current production from this complex totals 42 million stock-tank barrels (MMSTB) of an estimated 219 MMSTB of original oil in place, with an estimated 90 MMSTB of remaining mobile oil (RMO). The gross pay interval contains two parasequences consisting of progradational, 30-ft-thick, upward-shoaling facies packages. Facies include shoreface, mixed tidal channel and intertidal flat, and supratidal. Elongate shoreface facies are characterized by poorly consolidated, massive to thinly laminated sandstones. The supratidal facies, which act as permeability barriers, are characterized by algal-laminated dolostone and nodular, laminated, and massive anhydrite containing halite and gypsum pseudomorphs. Highest production and the largest amount of the 90 MMSTB of RMO is associated with the shoreface and tidal-channel facies. Bulk pore volume storage capacity and permeability are also highest within these two facies. Sandstones are arkosic, containing anhydrite and dolomite cements. Accessory minerals are clays, authigenic feldspar, and dolomite. Three main pore types are recognized: interparticle, moldic and intraconstituent, and micropores. Moldic and intraconstituent porosity is associated with leached feldspars and anhydrite cement dissolution. Microporosity is associated with syndepositional, grain-coating corrensite, dissolution-enhanced feldspar cleavage planes, and authigenic multifaceted dolomite. Microporosity derived from clays and dolomite is formed preferentially in tidal-channel and intertidal flat facies.

  4. Building America Case Study: Apartment Compartmentalization with an Aerosol-Based Sealing Process - Queens, NY; Technology Solutions for New and Existing Homes, Energy Efficiency & Renewable Energy (EERE)

    SciTech Connect (OSTI)

    2015-07-01

    Air sealing of building enclosures is a difficult and time-consuming process. Current methods in new construction require laborers to physically locate small and sometimes large holes in multiple assemblies and then manually seal each of them. The innovation demonstrated under this research study was the automated air sealing and compartmentalization of buildings through the use of an aerosolized sealant, developed by the Western Cooling Efficiency Center at University of California Davis.
    CARB sought to demonstrate this new technology application in a multifamily building in Queens, NY. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before and after sealing, point-source testing of individual leaks, and pressure measurements in the walls of the target apartment during sealing. Aerosolized sealing was successful by several measures in this study. Many individual leaks that are labor-intensive to address separately were well sealed by the aerosol particles. In addition, many diffuse leaks that are difficult to identify and treat were also sealed. The aerosol-based sealing process resulted in an average reduction of 71% in air leakage across three apartments and an average apartment airtightness of 0.08 CFM50/SF of enclosure area.

  5. The Structure and Function of an Arabinan-specific [alpha]-1,2-Arabinofuranosidase Identified from Screening the Activities of Bacterial GH43 Glycoside Hydrolases

    SciTech Connect (OSTI)

    Cartmell, Alan; McKee, Lauren S.; Pena, Maria J.; Larsbrink, Johan; Brumer, Harry; Kaneko, Satoshi; Ichinose, Hitomi; Lewis, Richard J.; Vikso-Nielsen, Anders; Gilbert, Harry; Marles-Wright, Jon

    2012-03-26

    Reflecting the diverse chemistry of plant cell walls, microorganisms that degrade these composite structures synthesize an array of glycoside hydrolases. These enzymes are organized into sequence-, mechanism-, and structure-based families. Genomic data have shown that several organisms that degrade the plant cell wall contain a large number of genes encoding family 43 (GH43) glycoside hydrolases. Here we report the biochemical properties of the GH43 enzymes of a saprophytic soil bacterium, Cellvibrio japonicus, and a human colonic symbiont, Bacteroides thetaiotaomicron. The data show that C. japonicus uses predominantly exo-acting enzymes to degrade arabinan into arabinose, whereas B. thetaiotaomicron deploys a combination of endo- and side chain-cleaving glycoside hydrolases. Both organisms, however, utilize an arabinan-specific {alpha}-1,2-arabinofuranosidase in the degradative process, an activity that has not previously been reported. The enzyme can cleave {alpha}-1,2-arabinofuranose decorations in single or double substitutions, the latter being recalcitrant to the action of other arabinofuranosidases. The crystal structure of the C. japonicus arabinan-specific {alpha}-1,2-arabinofuranosidase, CjAbf43A, displays a five-bladed {beta}-propeller fold. The specificity of the enzyme for arabinan is conferred by a surface cleft that is complementary to the helical backbone of the polysaccharide. The specificity of CjAbf43A for {alpha}-1,2-L-arabinofuranose side chains is conferred by a polar residue that orientates the arabinan backbone such that O2 arabinose decorations are directed into the active site pocket. A shelflike structure adjacent to the active site pocket accommodates O3 arabinose side chains, explaining how the enzyme can target O2 linkages that are components of single or double substitutions.

  6. A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK

    SciTech Connect (OSTI)

    Foster, J.W.; Schafer, A.J.; Critcher, R.

    1996-04-15

    We have constructed a whole genome radiation hybrid (WG-RH) map across a region of human chromosome 17q, from growth hormone (GH) to thymidine kinase (TK). A panel of 128 WG-RH hybrid cell lines generated by X-irradiation and fusion has been tested for the retention of 39 sequence-tagged site (STS) markers by the polymerase chain reaction. This genome mapping technique has allowed the integration of existing VNTR and microsatellite markers with additional new markers and existing STS markers previously mapped to this region by other means. The WG-RH map includes eight expressed sequence tag (EST) and three anonymous markers developed for this study, together with 23 anonymous microsatellites and five existing ESTs. Analysis of these data resulted in a high-density comprehensive map across this region of the genome. A subset of these markers has been used to produce a framework map consisting of 20 loci ordered with odds greater than 1000:1. The markers are of sufficient density to build a YAC contig across this region based on marker content. We have developed sequence tags for both ends of a 2.1-Mb YAC and mapped these using the WG-RH panel, allowing a direct comparison of cRay{sub 6000} to physical distance. 31 refs., 3 figs., 2 tabs.

  7. McQueen_1969.pdf

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

  8. 20140422 GH NERSC.key

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    detectors. - Add a fourth end station. * First beam after upgrade was a few weeks ago. ... reconstruction luminosity up to 10 particle ID 12 GeV Approved Experiments by Physics ...

  9. Desert Queen Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    Well Name: Location: Depth: Initial Flow Rate: "cf" is not declared as a valid unit of measurement for this property. The given value was not understood. Flow Test Comment:...

  10. Desert Queen Geothermal Area | Open Energy Information

    Open Energy Info (EERE)

    Well Name: Location: Depth: Initial Flow Rate: "b" is not declared as a valid unit of measurement for this property. The given value was not understood. Flow Test Comment:...

  11. Origin And Characterization Of Geothermal Waters At Desert Queen...

    Open Energy Info (EERE)

    energy potential. Further investigation by drilling is necessary to determine the true nature of the waters at depth. Authors Laura Garchar and Greg Arehart Published GRC, 2008 DOI...

  12. Queens County, New York: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AirShares EU Carbon Allowances Fund Allco Renewable Energy Group Limited LLC AltPower Inc Alternative Fuels Vehicle Group Ambata Capital Partners American Wind Power Hydrogen LLC...

  13. Sandia National Laboratories: Rodeo queen and chemical engineer

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    is driving them to the next rodeo, a loaded horse trailer hitched to the truck, chemical engineering textbooks strewn over the dash. "It's a crazy-busy life right now," says Cami,...

  14. Queen Creek, Arizona: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Creek, Arizona: Energy Resources Jump to: navigation, search Equivalent URI DBpedia Coordinates 33.2486638, -111.6342993 Show Map Loading map... "minzoom":false,"mappingservic...

  15. Final Report DE-GH 36-02GO12033

    SciTech Connect (OSTI)

    Kershaw, David

    2003-12-10

    The National Alliance of Clean Energy Business Incubators, established in 2000 by the National Renewable Energy Laboratory (NREL), is an emerging network of select business incubators providing business and financial services to clean energy entrepreneurs.

  16. EERE Success Story-ORNL Revitalizes Heating Process for Queen City

    Energy Savers [EERE]

    More Than 5% | Department of Energy New Tire Technologies Can Improve Fuel Efficiency by More Than 5% EERE Success Story-New Tire Technologies Can Improve Fuel Efficiency by More Than 5% January 15, 2016 - 10:17am Addthis EERE Success Story—New Tire Technologies Can Improve Fuel Efficiency by More Than 5% EERE Success Story—New Tire Technologies Can Improve Fuel Efficiency by More Than 5% EERE Success Story-New Tire Technologies Can Improve Fuel Efficiency by More Than 5% EERE

  17. Aerosol Observing System Greenhouse Gas (AOS GhG) Handbook (Technical...

    Office of Scientific and Technical Information (OSTI)

    tower through a series of control and monitoring components, and (2) the Picarro model ... Sponsoring Org: USDOE Office of Science (SC), Biological and Environmental Research (BER) ...

  18. MHK ISDB/Instruments/AirMar GH2183 GPS & Heading | Open Energy...

    Open Energy Info (EERE)

    Velocity Planar Measurement (Current), 3D Velocity Volumetric Measurement (Current), Density (Ice), Direction (Ice), Speed (Ice), Thickness (Ice), Pressure (Tidal), Sea Surface...

  19. H2A Delivery: GH2 and LH2 Forecourt Land Areas

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Setback distances specified by NFPA 55 Compressed Hydrogen Wall Opening: ... (wi ) 5 5 5 5 5 5 5 5 15 15 15 15 i m m m 2 99 115 115 130 1 1 2 3 ) m 2 1112 1112 1112 ...

  20. H2A Delivery: GH2 and LH2 Forecourt Land Areas

    Broader source: Energy.gov [DOE]

    Presentation by Matthew Hooks of TIAX at the Joint Meeting on Hydrogen Delivery Modeling and Analysis, May 8-9, 2007

  1. Romania: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Country Profile Name Romania Population 20,121,641 GDP 191,581,000,000 Energy Consumption 1.68 Quadrillion Btu 2-letter ISO code RO 3-letter ISO code ROU Numeric ISO...

  2. Experimental Characterization | Center for Gas SeparationsRelevant...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Experimental Characterization Previous Next List CraigBrown Craig Brown Jeff Kortright Jeffrey Kortright Queen Wendy Queen HewlittAugust2013 Jeffrey Reimer Simon Teat Simon Teat...

  3. R

    Office of Legacy Management (LM)

    ... 9rouP. nJ-purpol" ot-irtilssessnent ls to'verlfv tll da ?:p?*lng-te ade- qil.y-of tte rsnedlal rctlon rnd to confl-rn the slters ccrnpllrnce rlth rgnedlal rctlon crlterf a. ...

  4. Browse by Discipline -- E-print Network Subject Pathways: Physics...

    Office of Scientific and Technical Information (OSTI)

    - Department of Chemical Engineering, Queen's University (Kingston) Waldron, Kenneth J. (Kenneth J. Waldron) - Department of Mechanical Engineering, Stanford University Waleffe, ...

  5. TRUTeamWorks 07-08-04

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Ron Crockett, a local expert on bees, later determined that they were not Africanized bees, but rather regular honeybees that were particularly aggressive because the queen bee was ...

  6. Cass County, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Subtype A. Places in Cass County, Texas Atlanta, Texas Avinger, Texas Bloomburg, Texas Domino, Texas Douglassville, Texas Hughes Springs, Texas Linden, Texas Marietta, Texas Queen...

  7. Sub-nA spatially resolved conductivity profiling of surface and...

    Office of Scientific and Technical Information (OSTI)

    V. 4 ; Arruda, Thomas M. 5 + Show Author Affiliations Centre for Nanostructured Media, School of Mathematics and Physics, Queen's University Belfast, Belfast BT7 1NN (United ...

  8. Talbot County, Maryland: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Zone Subtype A. Places in Talbot County, Maryland Cordova, Maryland Easton, Maryland Oxford, Maryland Queen Anne, Maryland St. Michaels, Maryland Tilghman Island, Maryland...

  9. News Item

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Wendy Queen A fundamental materials challenge is to form structure that is not frozen in place but instead reconfigures internally driven by energy throughput and adapts to its...

  10. Enhancement of Aluminum Alloy Forgings Using Rapid Infrared Heating...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    and industry partners, Queen City Forging Company and Infra Red Heating Technologies LLC, have developed a process for forging aluminum parts using infrared (IR) technology. ...

  11. Browse by Discipline -- E-print Network Subject Pathways: Physics...

    Office of Scientific and Technical Information (OSTI)

    Queen Mary, University of London, School of Electronic Engineering and Computer Science, Antennas & Electromagnetics Group Queensland, University of - Department of Physics, Laser ...

  12. Quadrennial Energy Review Second Installment Electricity: Generation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Efforts are underway include the Con Edison BrooklynQueens project, which seeks to defer 1 billion in traditional distribution system upgrades with a 200 million investment in ...

  13. Highlights of SunShot Projects_Interconnection as Part of a Strategic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... So that's Brooklyn, Bronx, Manhattan, Queens, Staten Island, and Westchester. Those are the rows indicating the installations in each borough. And then just on the bottom that's ...

  14. Insecticide Transfer Efficiency and Lethal Load in Argentine Ants

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hooper-Bui, L. M.; Kwok, E S.C.; Buchholz, B. A.; Rust, M. K.; Eastmond, D. A.; Vogel, J. S.

    2015-07-03

    Trophallaxis between individual worker ants and the toxicant load in dead and live Argentine ants (Linepithema humile) in colonies exposed to fipronil and hydramethylnon experimental baits were examined using accelerator mass spectrometry (AMS). About 50% of the content of the crop containing trace levels of 14C-sucrose, 14C-hydramethylnon, and 14C-fipronil was shared between single donor and recipient ants. Dead workers and queens contained significantly more hydramethylnon (122.7 and 22.4 amol/μg ant, respectively) than did live workers and queens (96.3 and 10.4 amol/μg ant, respectively). Dead workers had significantly more fipronil (420.3 amol/μg ant) than did live workers (208.5 amol/μg ant), butmore » dead and live queens had equal fipronil levels (59.5 and 54.3 amol/μg ant, respectively). Moreover, the distribution of fipronil differed within the bodies of dead and live queens; the highest amounts of fipronil were recovered in the thorax of dead queens whereas live queens had the highest levels in the head. Resurgence of polygynous ant colonies treated with hydramethylnon baits may be explained by queen survival resulting from sublethal doses due to a slowing of trophallaxis throughout the colony. The bait strategies and dose levels for controlling insect pests need to be based on the specific toxicant properties and trophic strategies for targeting the entire colony.« less

  15. Insecticide Transfer Efficiency and Lethal Load in Argentine Ants

    SciTech Connect (OSTI)

    Hooper-Bui, L. M.; Kwok, E S.C.; Buchholz, B. A.; Rust, M. K.; Eastmond, D. A.; Vogel, J. S.

    2015-07-03

    Trophallaxis between individual worker ants and the toxicant load in dead and live Argentine ants (Linepithema humile) in colonies exposed to fipronil and hydramethylnon experimental baits were examined using accelerator mass spectrometry (AMS). About 50% of the content of the crop containing trace levels of 14C-sucrose, 14C-hydramethylnon, and 14C-fipronil was shared between single donor and recipient ants. Dead workers and queens contained significantly more hydramethylnon (122.7 and 22.4 amol/μg ant, respectively) than did live workers and queens (96.3 and 10.4 amol/μg ant, respectively). Dead workers had significantly more fipronil (420.3 amol/μg ant) than did live workers (208.5 amol/μg ant), but dead and live queens had equal fipronil levels (59.5 and 54.3 amol/μg ant, respectively). Moreover, the distribution of fipronil differed within the bodies of dead and live queens; the highest amounts of fipronil were recovered in the thorax of dead queens whereas live queens had the highest levels in the head. Resurgence of polygynous ant colonies treated with hydramethylnon baits may be explained by queen survival resulting from sublethal doses due to a slowing of trophallaxis throughout the colony. The bait strategies and dose levels for controlling insect pests need to be based on the specific toxicant properties and trophic strategies for targeting the entire colony.

  16. Complexation of Gluconate with Uranium(VI) in Acidic Solutions: Thermodynamic Study with Structural Analysis

    SciTech Connect (OSTI)

    Zhang, Zhicheng; Helms, G.; Clark, S. B.; Tian, Guoxin; Zanonato, PierLuigi; Rao, Linfeng

    2009-01-05

    Within the pC{sub H} range of 2.5 to 4.2, gluconate forms three uranyl complexes UO{sub 2}(GH{sub 4}){sup +}, UO{sub 2}(GH{sub 3})(aq), and UO{sub 2}(GH{sub 3})(GH{sub 4}){sup -}, through the following reactions: (1) UO{sub 2}{sup 2+} + GH{sub 4}{sup -} = UO{sub 2}(GH{sub 4}){sup +}, (2) UO{sub 2}{sup 2+} + GH{sub 4}{sup -} = UO{sub 2}(GH{sub 3})(aq) + H{sup +}, and (3) UO{sub 2}{sup 2+} + 2GH{sub 4}{sup -} = UO{sub 2}(GH{sub 3})(GH{sub 4}){sup -} + H{sup +}. Complexes were inferred from potentiometric, calorimetric, NMR, and EXAFS studies. Correspondingly, the stability constants and enthalpies were determined to be log {Beta}{sub 1} = 2.2 {+-} 0.3 and {Delta}H{sub 1} = 7.5 {+-} 1.3 kJ mol{sup -1} for reaction (1), log {Beta}{sub 2} = -(0.38 {+-} 0.05) and {Delta}H{sub 2} = 15.4 {+-} 0.3 kJ mol{sup -1} for reaction (2), and log {Beta}{sub 3} = 1.3 {+-} 0.2 and {Delta}H{sub 3} = 14.6 {+-} 0.3 kJ mol{sup -1} for reaction (3), at I = 1.0 M NaClO{sub 4} and t = 25 C. The UO{sub 2}(GH{sub 4}){sup +} complex forms through the bidentate carboxylate binding to U(VI). In the UO{sub 2}(GH{sub 3})(aq) complex, hydroxyl-deprotonated gluconate (GH{sub 3}{sup 2-}) coordinates to U(VI) through the five-membered ring chelation. For the UO{sub 2}(GH{sub 3})(GH{sub 4}){sup -} complex, multiple coordination modes are suggested. These results are discussed in the context of trivalent and pentavalent actinide complexation by gluconate.

  17. Slide 1

    Broader source: Energy.gov (indexed) [DOE]

    ... North Slope GH assessment 7. Continue to enable full international collaboration 1. Support for NGHP-Exp-02 (India) planning 2. Continue modeling collaborations with KIGAM (Korea) ...

  18. New Cellulase Identification Method Holds Promise for Lower-Cost Biofuels (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2013-04-01

    A new computational approach to genomic data effectively distinguishes cellulases and non-cellulases within the protein family GH48, a key component for degrading lignocellulose for biofuels.

  19. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... (4mC), will enable strategies to make these species genetically tractable. ... The C. bescii genome contains six GH5more domains in five different open reading ...

  20. Green Hydrogen Company | Open Energy Information

    Open Energy Info (EERE)

    Hydrogen Company Jump to: navigation, search Logo: Green Hydrogen Company Name: Green Hydrogen Company Abbreviation: GH2 Address: Green Hydrogen Company, Head Office, 9...

  1. PowerPoint Presentation

    Broader source: Energy.gov (indexed) [DOE]

    ECA Public Domain Interagency & International Merit-based & Transparent Gas Hydrate ... of exploration technologies - integration of GH science into climate change ...

  2. In the OSTI Collections: the Higgs Boson | OSTI, US Dept of Energy...

    Office of Scientific and Technical Information (OSTI)

    ...gh-energy physics laboratory, by collaborations of physicists from many countries around the world and sponsored by several organizations, including the U. S. Department of Energy. ...

  3. Named Fellowships Luminary - Walter H. Zinn | Argonne National...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    was born in December 1906 in Canada. Zinn graduated from Queen's University with a mathematics degree, and went on to do his Ph.D. in nuclear physics at Columbia University. He...

  4. Hydrocarbon Separations in a Metal-Organic Framework with Open...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydrocarbon Separations in a Metal-Organic Framework with Open Iron(II) Coordination Sites Previous Next List E. D. Bloch, W. L. Queen, R. Krishna, J. M. Zadrozny, C. M. Brown, and ...

  5. Simon Teat | Center for Gas SeparationsRelevant to Clean Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Single Crystalline Porphyrinic Titanium Metal-organic Framework. Chem. Sci., 6, 3926-3930 (2015). 10.1039c5sc00916b Queen, Wendy L; Hudson, Matthew R; Bloch, Eric D; Mason, ...

  6. Craig Brown | Center for Gas SeparationsRelevant to Clean Energy...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bloch, Eric D; Queen, Wendy L; Krishna, Rajamani; Zadrozny, Joseph M.; Brown, Craig M; and Long, Jeffrey R Hydrocarbon Separations in a Metal-Organic Framework with Open Iron(II) ...

  7. Selective Binding of O2 over N2 in a Redox-Active Metal-Organic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Selective Binding of O2 over N2 in a Redox-Active Metal-Organic Framework with Open Iron(II) Coordination Sites Previous Next List E. D. Bloch, L. J. Murray, W. L. Queen, S. ...

  8. Albany Center map_rev36-10

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bro adw ay Stre et Lib ert y Str ee t Queen Avenue N S1 S2 S3 Locked Gate Locked Gate Locked Gate Locked Gate Locked Gate Locked Gate Walk Gate (locked) Walk Gate (locked) Walk...

  9. BPA-2010-00989-F Correspondence

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    March 1, 2010 In reply refer to: DK-7 Ben Tansey, Sr. Contributing Editor Clearing Up, Energy News Data Publications P.O. Box 900928 Queen Anne Station Seattle, WA 98119-9228 FOIA...

  10. Browse by Discipline -- E-print Network Subject Pathways: Mathematics...

    Office of Scientific and Technical Information (OSTI)

    F G H I J K L M N O P Q R S T U V W X Y Z Elkashlan, Maged (Maged Elkashlan) - School of Electronic Engineering and Computer Science, Queen Mary, University of London Erdogan, ...

  11. NATIONAL LEAD COMPANY

    Office of Legacy Management (LM)

    OR ' wuo March 30, 1954 .suBJEcr c TWO Q-11 DRUMS RECONDITIONED AT QUEEN CITY BARREL CO.,,CINCINNATI TO Ft. C. Heatherton FROM E. v. BarTy On Monday, March 29, the writer and...

  12. CX-013382: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    McQueens Branch Hazard Mitigation CX(s) Applied: B3.1Date: 01/08/2015 Location(s): South CarolinaOffices(s): Savannah River Operations Office

  13. A meeting of the minds when NYC CoolRoofs visits PPPL | Princeton...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    which collected data on three white "cool roofs," including one on the Museum of Modern Art Queens in Long Island City, and found there was a 42 degree Fahrenheit difference...

  14. Solution-Processed Solar Cells using Colloidal Quantum Dots ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bio: Ted Sargent received the B.Sc.Eng. (Engineering Physics) from Queen's University in 1995 and the Ph.D. in Electrical and Computer Engineering (Photonics) from the University ...

  15. DOI-LM-NV-W010-2012-0061-CX | Open Energy Information

    Open Energy Info (EERE)

    LM-NV-W010-2012-0061-CX Jump to: navigation, search NEPA Document Collection for: DOI-LM-NV-W010-2012-0061-CX CX at Desert Queen Geothermal Area for GeothermalExploration, CX for...

  16. National Grid (Gas)- Residential Energy Efficiency Rebate Programs (Metro New York)

    Broader source: Energy.gov [DOE]

    National Grid’s High Efficiency Heating Rebates are offered to residential gas heating customers in the New York City metro area including Brooklyn, Queens, and Staten Island.  Rebates vary...

  17. STEAB December 2013 Meeting Minutes

    Broader source: Energy.gov (indexed) [DOE]

    ... FM continued saying solar is an issue in New York. There is a demand for solar output in certain areas of some boroughs like Brooklyn and Queens, but on the weekend those are ...

  18. Microsoft Word - 298471_2.DOCX

    Broader source: Energy.gov (indexed) [DOE]

    States border to a converter station in Astoria, Queens, New York. To minimize the ... Id. at p. 11,518. Question (5) seeks information on the length of time required to design, ...

  19. Comparison of pectin-degrading fungal communities in temperate forests using glycosyl hydrolase family 28 pectinase primers targeting Ascomycete fungi

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gacura, Matthew D.; Sprockett, Daniel D.; Heidenreich, Bess; Blackwood, Christopher B.

    2016-02-17

    Here, fungi have developed a wide assortment of enzymes to break down pectin, a prevalent polymer in plant cell walls that is important in plant defense and structure. One enzyme family used to degrade pectin is the glycosyl hydrolase family 28 (GH28). In this studywe developed primers for the amplification of GH28 coding genes from a database of 293 GH28 sequences from40 fungal genomes. The primerswere used to successfully amplify GH28 pectinases from all Ascomycota cultures tested, but only three out of seven Basidiomycota cultures. In addition, we further tested the primers in PCRs on metagenomic DNA extracted from senescedmore » tree leaves from different forest ecosystems, followed by cloning and sequencing. Taxonomic specificity for Ascomycota GH28 genes was tested by comparing GH28 composition in leaves to internal transcribed spacer (ITS) amplicon composition using pyrosequencing. All sequences obtained from GH28 primers were classified as Ascomycota; in contrast, ITS sequences indicated that fungal communitieswere up to 39% Basidiomycetes. Analysis of leaf samples indicated that both forest stand and ecosystemtype were important in structuring fungal communities. However, site played the prominent role in explaining GH28 composition, whereas ecosystem type was more important for ITS composition, indicating possible genetic drift between populations of fungi. Overall, these primers will have utility in understanding relationships between fungal community composition and ecosystem processes, as well as detection of potentially pathogenic Ascomycetes.« less

  20. Probing Electrons and Vibrations in Functional Materials | MIT-Harvard

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Center for Excitonics Electrons and Vibrations in Functional Materials October 20, 2015 at 4:30pm/Pappalardo Room: 4-349* Tyrel McQueen Department of Chemistry, Department of Materials Science and Engineering, Department of Physics and Astronomy, Institute for Quantum Matter, The Johns Hopkins University TyrelMcQueen Developing models to predict the behavior of new inorganic solids and design materials with desirable physical properties relies on accurate determination of structure,

  1. Arthur B. McDonald and Oscillating Neutrinos

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Arthur B. McDonald and Oscillating Neutrinos Resources with Additional Information Arthur B. McDonald Courtesy of Queen's University 'Queen's University professor emeritus Arthur McDonald is the co-winner of the 2015 Nobel Prize in physics. ... Dr. McDonald won the award, along with Takaaki Kajita of the University of Tokyo, "for their key contributions to the experiments which demonstrated that neutrinos change identities."... The findings solved a puzzle that physicists had wrestled

  2. Challenges, uncertainties and issues facing gas production from gas hydrate deposits

    SciTech Connect (OSTI)

    Moridis, G.J.; Collett, T.S.; Pooladi-Darvish, M.; Hancock, S.; Santamarina, C.; Boswell, R.; Kneafsey, T.; Rutqvist, J.; Kowalsky, M.; Reagan, M.T.; Sloan, E.D.; Sum, A.K.; Koh, C.

    2010-11-01

    The current paper complements the Moridis et al. (2009) review of the status of the effort toward commercial gas production from hydrates. We aim to describe the concept of the gas hydrate petroleum system, to discuss advances, requirement and suggested practices in gas hydrate (GH) prospecting and GH deposit characterization, and to review the associated technical, economic and environmental challenges and uncertainties, including: the accurate assessment of producible fractions of the GH resource, the development of methodologies for identifying suitable production targets, the sampling of hydrate-bearing sediments and sample analysis, the analysis and interpretation of geophysical surveys of GH reservoirs, well testing methods and interpretation of the results, geomechanical and reservoir/well stability concerns, well design, operation and installation, field operations and extending production beyond sand-dominated GH reservoirs, monitoring production and geomechanical stability, laboratory investigations, fundamental knowledge of hydrate behavior, the economics of commercial gas production from hydrates, and the associated environmental concerns.

  3. Compositions for enhancing hydroysis of cellulosic material by cellulolytic enzyme compositions

    SciTech Connect (OSTI)

    Quinlan, Jason; Xu, Feng; Sweeney, Matthew; Johansen, Katja Salomon

    2014-09-30

    The present invention relates to compositions comprising a GH61 polypeptide having cellulolytic enhancing activity and an organic compound comprising a carboxylic acid moiety, a lactone moiety, a phenolic moiety, a flavonoid moiety, or a combination thereof, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the organic compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme compared to the GH61 polypeptide alone or the organic compound alone. The present invention also relates to methods of using the compositions.

  4. Geology of Southwestern New Mexico | Open Energy Information

    Open Energy Info (EERE)

    to library Conference Paper: Geology of Southwestern New Mexico Authors R.E. Clemons and G.H. Mack Conference 39th Field Conference; ConferencePlace"ConferencePlace"...

  5. --No Title--

    U.S. Energy Information Administration (EIA) Indexed Site

    by Source, 2008 (Dollars per Million Btu) State Primary Energy Electric Power Sector g,h Retail Electricity Total Energy g,i Coal Natural Gas a Petroleum Nuclear Fuel Biomass...

  6. Measuring solar reflectance Part II: Review of practical methods...

    Office of Scientific and Technical Information (OSTI)

    solar position, and found that clear sky air mass 1 global horizontal (AM1GH) solar ... Rsub g,0more to within 0.006. The air mass 1.5 solar reflectance measured with ...

  7. Resonant Soft X-Ray Scattering of Tri-Block Copolymers

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    enormous parameter space for the creation of new morphologies. Illustration from F.S. Bates and G.H. Fredrickson, "Block copolymers-designer soft materials," Physics Today 52, 32...

  8. CX-013489: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Maintenance Clean the Big Hill Raw Water Intake Structure Pump Bay (GH-OM-1167) CX(s) Applied: B1.3Date: 02/23/2015 Location(s): TexasOffices(s): Strategic Petroleum Reserve Field Office

  9. High-Yield Hybrid Cellulosic Ethanol

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hig gh-Yield Hy ybrid Cellulosic Ethanol Process Using High-Impact Feedstock WBS 5.5.11.1 ... Markets Poplar C2 Platform End Markets Ethanol Acetic Acid Ethylene Vinyl Acetate 2 ...

  10. PNWD

    Office of Legacy Management (LM)

    ... increa ses in selenium in sur face water and sed iment that can affect fish and wildl ife (particularly avian species) from the co nsum ptio n o f selenium throu gh the food web. ...

  11. Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis

    SciTech Connect (OSTI)

    Yu X. H.; Shanklin J.; Rawat, R.

    2011-05-01

    Cyclopropane fatty acids (CPA) have been found in certain gymnosperms, Malvales, Litchi and other Sapindales. The presence of their unique strained ring structures confers physical and chemical properties characteristic of unsaturated fatty acids with the oxidative stability displayed by saturated fatty acids making them of considerable industrial interest. While cyclopropenoid fatty acids (CPE) are well-known inhibitors of fatty acid desaturation in animals, CPE can also inhibit the stearoyl-CoA desaturase and interfere with the maturation and reproduction of some insect species suggesting that in addition to their traditional role as storage lipids, CPE can contribute to the protection of plants from herbivory. Three genes encoding cyclopropane synthase homologues GhCPS1, GhCPS2 and GhCPS3 were identified in cotton. Determination of gene transcript abundance revealed differences among the expression of GhCPS1, 2 and 3 showing high, intermediate and low levels, respectively, of transcripts in roots and stems; whereas GhCPS1 and 2 are both expressed at low levels in seeds. Analyses of fatty acid composition in different tissues indicate that the expression patterns of GhCPS1 and 2 correlate with cyclic fatty acid (CFA) distribution. Deletion of the N-terminal oxidase domain lowered GhCPS's ability to produce cyclopropane fatty acid by approximately 70%. GhCPS1 and 2, but not 3 resulted in the production of cyclopropane fatty acids upon heterologous expression in yeast, tobacco BY2 cell and Arabidopsis seed. In cotton GhCPS1 and 2 gene expression correlates with the total CFA content in roots, stems and seeds. That GhCPS1 and 2 are expressed at a similar level in seed suggests both of them can be considered potential targets for gene silencing to reduce undesirable seed CPE accumulation. Because GhCPS1 is more active in yeast than the published Sterculia CPS and shows similar activity when expressed in model plant systems, it represents a strong candidate gene for

  12. New Cellulase Identification Method Holds Promise for Lower-Cost Biofuels (Fact Sheet), NREL (National Renewable Energy Laboratory)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A new computational approach to genomic data effectively distinguishes cellulases and non-cellulases within the protein family GH48, a key component for degrading lignocellulose for biofuels. Cellulase enzymes, particularly from the glycoside hydrolase family 48 (GH48), are a critical component of many lignocellulose-degrading systems that produce biomass to use for biofu- els. Typically it has been difficult and costly-but necessary-to distinguish required cellulase enzymes from unwanted

  13. International Workshop: MFE Roadmapping in the ITER Era | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab Princeton, NJ International Workshop: MFE Roadmapping in the ITER Era Princeton University Princeton, NJ Host: G.H. Neilson Coordinator: Pamela Hampton /p> Contact Information Website: Website Coordinator(s): Pamela Hampton phampton@pppl.gov Host(s): G.H. Neilson hneilson@pppl.gov PPPL Entrance Procedures Visitor Information, Directions, Security at PPPL As a federal facility, the Princeton Plasma Physics Laboratory is operating under heightened security measures because of

  14. Microsoft PowerPoint - To NETL Albany Site from Eugene, Oregon Airport Directions.ppt [Compatibility Mode]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Eugene, Oregon Airport 1. From the EUGENE AIRPORT take HWY 99 (the airport is located off Hwy 99). 2. Follow HWY 99 NORTH from EUGENE to ALBANY. 3. Outside of EUGENE, HWY 99 splits into HWY 99 EAST and 99 WEST. 4. Take HWY 99 EAST to ALBANY (bear right at intersection). 5. You are nearing ALBANY when you pass under HWY 34. 6. Continue on 99 EAST, PACIFIC BLVD., until it intersects QUEEN AVENUE (there will be a directional sign at intersection for Albany Site). 7. Turn LEFT (WEST) on QUEEN

  15. Microsoft PowerPoint - To NETL Albany Site from Portland, Oregon Airport (PDX) Directions.ppt [Compatibility Mode]

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Portland, Oregon Airport (PDX) 1. Take the AIRPORT EXIT RD. until it intersects I-205. 2. Follow I-205 SOUTH for 25 MILES to the intersection with I-5 SOUTH (Salem exit). 3. Follow I-5 SOUTH for approximately 60 miles to the 1 st Albany exit, EXIT 234B - ALBANY, PACIFIC BLVD, OREGON HIGHWAY 99. 4. Follow PACIFIC BLVD. to QUEEN AVE. 5. TURN RIGHT (WEST) on QUEEN AVE. 6 The ALBANY SITE is located on the LEFT just past WEST ALBANY HIGH SCHOOL 6. The ALBANY SITE is located on the LEFT just past WEST

  16. Study on Evaluation of Project Management Data for Decommissioning of Uranium Refining and Conversion Plant - 12234

    SciTech Connect (OSTI)

    Usui, Hideo; Izumo, Sari; Tachibana, Mitsuo; Shibahara, Yuji; Morimoto, Yasuyuki; Tokuyasu, Takashi; Takahashi, Nobuo; Tanaka, Yoshio; Sugitsue, Noritake

    2012-07-01

    Some of nuclear facilities that would no longer be required have been decommissioned in JAEA (Japan Atomic Energy Agency). A lot of nuclear facilities have to be decommissioned in JAEA in near future. To implement decommissioning of nuclear facilities, it was important to make a rational decommissioning plan. Therefore, project management data evaluation system for dismantling activities (PRODIA code) has been developed, and will be useful for making a detailed decommissioning plan for an object facility. Dismantling of dry conversion facility in the uranium refining and conversion plant (URCP) at Ningyo-toge began in 2008. During dismantling activities, project management data such as manpower and amount of waste generation have been collected. Such collected project management data has been evaluated and used to establish a calculation formula to calculate manpower for dismantling equipment of chemical process and calculate manpower for using a green house (GH) which was a temporary structure for preventing the spread of contaminants during dismantling. In the calculation formula to calculate project management data related to dismantling of equipment, the relation of dismantling manpower to each piece of equipment was evaluated. Furthermore, the relation of dismantling manpower to each chemical process was evaluated. The results showed promise for evaluating dismantling manpower with respect to each chemical process. In the calculation formula to calculate project management data related to use of the GH, relations of GH installation manpower and removal manpower to GH footprint were evaluated. Furthermore, the calculation formula for secondary waste generation was established. In this study, project management data related to dismantling of equipment and use of the GH were evaluated and analyzed. The project management data, manpower for dismantling of equipment, manpower for installation and removal of GH, and secondary waste generation from GH were considered

  17. Presence of specific growth hormone binding sites in rainbow trout (Oncorhynchus mykiss) tissues: characterization of the hepatic receptor

    SciTech Connect (OSTI)

    Yao, K.; Niu, P.D.; Le Gac, F.; Le Bail, P.Y. )

    1991-01-01

    The present work outlines the presence of specific binding for chinook salmon growth hormone (sGH) in different tissue preparations of rainbow trout. Optimal incubation conditions (pH, Tris, MgCl{sub 2}) were determined. Specific binding was very sensitive to salt concentration during incubation. The specific binding reached a plateau after 15 and 25 hr of incubation at 12 and 4 {degree}. At 20 {degree}, specific and nonspecific binding were not stable. Specific binding dissociation was slower than association and was only partial. The binding was saturable (Bmax = 187 +/- 167 pmol), of high affinity (Ka = 2.4 +/- 0.8 10(9) M-1), and very specific for GH, properties which are in agreement with the characteristics of hormonal receptors. Sea bream and mammalian GH appeared 2- and 30-fold, respectively, less potent than cold sGH2 for displacing {sup 125}I-sGH2. Tissue preparations from ovary, testis, fat, skin, cartilage, gill, blood pellet, brain, spleen, kidney, and muscle showed significant saturable binding.

  18. Hydrogen Delivery Options and Issues

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Options and Issues Mark Paster DOE August, 2006 Scope * From the end point of central or distributed production (300 psi H2) to and including the dispenser at a refueling station or stationary power site - GH2 Pipelines and Trucks, LH2 Trucks, Carriers <$1.00/kg of Hydrogen by 2017 Hydrogen Delivery H2 Delivery Current Status * Technology - GH2 Tube Trailers: ~340 kg, ~2600 psi - LH2 Trucks: ~3900 kg - Pipelines: up to 1500 psi (~630 miles in the U.S.) - Refueling Site Operations

  19. Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community

    SciTech Connect (OSTI)

    Allgaier, M.; Reddy, A.; Park, J. I.; Ivanova, N.; D'haeseleer, P.; Lowry, S.; Sapra, R.; Hazen, T.C.; Simmons, B.A.; VanderGheynst, J. S.; Hugenholtz, P.

    2009-11-15

    Development of cellulosic biofuels from non-food crops is currently an area of intense research interest. Tailoring depolymerizing enzymes to particular feedstocks and pretreatment conditions is one promising avenue of research in this area. Here we added a green-waste compost inoculum to switchgrass (Panicum virgatum) and simulated thermophilic composting in a bioreactor to select for a switchgrass-adapted community and to facilitate targeted discovery of glycoside hydrolases. Small-subunit (SSU) rRNA-based community profiles revealed that the microbial community changed dramatically between the initial and switchgrass-adapted compost (SAC) with some bacterial populations being enriched over 20-fold. We obtained 225 Mbp of 454-titanium pyrosequence data from the SAC community and conservatively identified 800 genes encoding glycoside hydrolase domains that were biased toward depolymerizing grass cell wall components. Of these, {approx}10% were putative cellulases mostly belonging to families GH5 and GH9. We synthesized two SAC GH9 genes with codon optimization for heterologous expression in Escherichia coli and observed activity for one on carboxymethyl cellulose. The active GH9 enzyme has a temperature optimum of 50 C and pH range of 5.5 to 8 consistent with the composting conditions applied. We demonstrate that microbial communities adapt to switchgrass decomposition using simulated composting condition and that full-length genes can be identified from complex metagenomic sequence data, synthesized and expressed resulting in active enzyme.

  20. Targeted Discovery of Glycoside Hydrolases from a Switchgrass-Adapted Compost Community

    SciTech Connect (OSTI)

    Reddy, Amitha; Allgaier, Martin; Park, Joshua I.; Ivanoval, Natalia; Dhaeseleer, Patrik; Lowry, Steve; Sapra, Rajat; Hazen, Terry C.; Simmons, Blake A.; VanderGheynst, Jean S.; Hugenholtz, Philip

    2011-05-11

    Development of cellulosic biofuels from non-food crops is currently an area of intense research interest. Tailoring depolymerizing enzymes to particular feedstocks and pretreatment conditions is one promising avenue of research in this area. Here we added a green-waste compost inoculum to switchgrass (Panicum virgatum) and simulated thermophilic composting in a bioreactor to select for a switchgrass-adapted community and to facilitate targeted discovery of glycoside hydrolases. Smallsubunit (SSU) rRNA-based community profiles revealed that the microbial community changed dramatically between the initial and switchgrass-adapted compost (SAC) with some bacterial populations being enriched over 20-fold. We obtained 225 Mbp of 454-titanium pyrosequence data from the SAC community and conservatively identified 800 genes encoding glycoside hydrolase domains that were biased toward depolymerizing grass cell wall components. Of these, ,10percent were putative cellulasesmostly belonging to families GH5 and GH9. We synthesized two SAC GH9 genes with codon optimization for heterologous expression in Escherichia coli and observed activity for one on carboxymethyl cellulose. The active GH9 enzyme has a temperature optimum of 50uC and pH range of 5.5 to 8 consistent with the composting conditions applied. We demonstrate that microbial communities adapt to switchgrass decomposition using simulated composting condition and that full-length genes can be identified from complex metagenomic sequence data, synthesized and expressed resulting in active enzyme.

  1. Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chung, Daehwan; Young, Jenna; Cha, Minseok; Brunecky, Roman; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2015-08-13

    The Caldicellulosiruptor bescii genome encodes a potent set of carbohydrate-active enzymes (CAZymes), found primarily as multi-domain enzymes that exhibit high cellulolytic and hemicellulolytic activity on and allow utilization of a broad range of substrates, including plant biomass without conventional pretreatment. CelA, the most abundant cellulase in the C. bescii secretome, uniquely combines a GH9 endoglucanase and a GH48 exoglucanase in one protein. The most effective commercial enzyme cocktails used in vitro to pretreat biomass are derived from fungal cellulases (cellobiohydrolases, endoglucanases and a β-d-glucosidases) that act synergistically to release sugars for microbial conversion. The C. bescii genome contains six GH5more » domains in five different open reading frames. Four exist in multi-domain proteins and two as single catalytic domains. E1 is a GH5 endoglucanase reported to have high specific activity and simple architecture and is active at the growth temperature of C. bescii. E1 is an endo-1,4-β-glucanase linked to a family 2 carbohydrate-binding module shown to bind primarily to cellulosic substrates. As a result, we tested if the addition of this protein to the C. bescii secretome would improve its cellulolytic activity.« less

  2. Expression of the Acidothermus cellulolyticus E1 endoglucanase in Caldicellulosiruptor bescii enhances its ability to deconstruct crystalline cellulose

    SciTech Connect (OSTI)

    Chung, Daehwan; Young, Jenna; Cha, Minseok; Brunecky, Roman; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2015-08-13

    The Caldicellulosiruptor bescii genome encodes a potent set of carbohydrate-active enzymes (CAZymes), found primarily as multi-domain enzymes that exhibit high cellulolytic and hemicellulolytic activity on and allow utilization of a broad range of substrates, including plant biomass without conventional pretreatment. CelA, the most abundant cellulase in the C. bescii secretome, uniquely combines a GH9 endoglucanase and a GH48 exoglucanase in one protein. The most effective commercial enzyme cocktails used in vitro to pretreat biomass are derived from fungal cellulases (cellobiohydrolases, endoglucanases and a β-d-glucosidases) that act synergistically to release sugars for microbial conversion. The C. bescii genome contains six GH5 domains in five different open reading frames. Four exist in multi-domain proteins and two as single catalytic domains. E1 is a GH5 endoglucanase reported to have high specific activity and simple architecture and is active at the growth temperature of C. bescii. E1 is an endo-1,4-β-glucanase linked to a family 2 carbohydrate-binding module shown to bind primarily to cellulosic substrates. As a result, we tested if the addition of this protein to the C. bescii secretome would improve its cellulolytic activity.

  3. DOE Hydrogen Transition Analysis Workshop: Final Agenda

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    T Th hu ur rs sd da ay y ( (R Ro oo om m G GH H- -0 01 19 ... am HyTrans Model - David Greene, ORNL 10:10 am Market ... Marianne Mintz (ANL), and Mark Ruth (NREL) 12:00 pm Lunch ...

  4. Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus

    DOE Patents [OSTI]

    Adney, William S.; Vinzant, Todd B.; Ding, Shih-You; Himmel, Michael E.

    2011-04-26

    The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

  5. Thermal tolerant avicelase from Acidothermus cellulolyticus

    DOE Patents [OSTI]

    Ding, Shi-You; Adney, William S.; Vinzant, Todd B.; Himmel, Michael E.

    2009-05-26

    The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

  6. Thermal tolerant avicelase from Acidothermus cellulolyticus

    DOE Patents [OSTI]

    Ding, Shi-You; Adney, William S.; Vinzant, Todd B.; Himmel, Michael E.

    2008-04-29

    The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

  7. International Workshop: MFE Roadmapping in the ITER Era | Princeton Plasma

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Lab International Workshop: MFE Roadmapping in the ITER Era International Workshop: MFE Roadmapping in the ITER Era Contact Information Website: International Workshop: MFE Roadmapping in the ITER Era Coordinator(s): Pamela Hampton phampton@pppl.gov Host(s): G.H. Neilson hneilson@pppl.gov Learn More Fusion roadmapping

  8. Refueliing Infrastructure for Alternative Fuel Vehicles: Lessons...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    st tr ra at ti io on n a an nd d N Ne et tw wo or rk ki in ng g R Re ec ce ep pt ti io on n ( (l li ig gh ht t f fa ar re e) ) THURSDAY, APRIL 3 7:00 am R Re eg gi is st tr ra at ...

  9. Transportation and Stationary Power Integration: Workshop Proceedings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transportation and Stationary Power Integration WORKSHOP PROCEEDINGS Phoenix, Arizona October 27, 2008 Acknowledgements This workshop was planned under the guidance of Marc Melaina of the National Renewable Energy Laboratory (NREL) and Fred Joseck of the Department of Energy's Hydrogen, Fuel Cells & Infrastructure Technologies Program. Workshop organization and facilitation was provided by Energetics, Incorporated in Columbia, Maryland. Breakout group facilitators included Shawna McQueen,

  10. Seismic Fracture Characterization Methods for Enhanced Geothermal Systems |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Systems Seismic Fracture Characterization Methods for Enhanced Geothermal Systems Project objective: Make Seismic Work in Geothermal Areas; Characterize Fractures/Faults. seismic_queen_seismic_fracture.pdf (1.38 MB) More Documents & Publications Imaging, Characterizing, and Modeling of Fracture Networks and Fluid Flow in EGS Reservoirs; II: Full-Waveform Inversion of 3D-9C VSP data from Bradys EGS Site and Update of the Brady Reservoir Scale Model Imaging,

  11. Seismic Fracture Characterization Methods for Enhanced Geothermal Systems;

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2010 Geothermal Technology Program Peer Review Report | Department of Energy Systems; 2010 Geothermal Technology Program Peer Review Report Seismic Fracture Characterization Methods for Enhanced Geothermal Systems; 2010 Geothermal Technology Program Peer Review Report DOE 2010 Geothermal Technologies Program Peer Review seismic_022_queen.pdf (195.2 KB) More Documents & Publications Microearthquake Technology for EGS Fracture Characterization; 2010 Geothermal Technology Program Peer

  12. Sandia National Laboratories:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    07, 2016 Articles RESEARCHER JUAN ELIZONDO-DECANINI (2624) holds two compact, high-voltage nonlinear transmission lines. Got Solitons? Sandia researcher sees problem as a solution Photo courtesy of Cami Belcher Rodeo queen and chemical engineer Hystep device speeds H2 station commissioning HyStEP device speeds H2 refueling station commissioning Sandia researcher Charles Reinke studies a tiny phononic/photonic filter on a green, stamp-sized substrate toward the picture's bottom-right. New

  13. Refueling Infrastructure for Alternative Fuel Vehicles: Lessons Learned for Hydrogen; Workshop Proceedings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Refueling Infrastructure for Alternative Fuel Vehicles: Lessons Learned for Hydrogen Workshop Proceedings M.W. Melaina National Renewable Energy Laboratory S. McQueen and J. Brinch Energetics Incorporated Sacramento, California April 3, 2008 Proceedings NREL/BK-560-43669 July 2008 NREL is operated by Midwest Research Institute ● Battelle Contract No. DE-AC36-99-GO10337 Refueling Infrastructure for Alternative Fuel Vehicles: Lessons Learned for Hydrogen Workshop Proceedings M.W. Melaina

  14. Control of Light-matter Interaction Using Dispersion Engineered Photonic

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structures | MIT-Harvard Center for Excitonics Control of Light-matter Interaction Using Dispersion Engineered Photonic Structures March 8, 2011 at 3pm/36-428 Vinod M. Menon Queens College of the City University of New York (CUNY) menon_000 abstract: Coherent interaction of an ensemble of dipole active atoms or excitons with vacuum electromagnetic field has been studied extensively since its initial conception by Dicke in 1954. However, when the emitters are not only periodically arranged,

  15. Hydrogen Infrastructure Market Readiness: Opportunities and Potential for Near-term Cost Reductions. Proceedings of the Hydrogen Infrastructure Market Readiness Workshop and Summary of Feedback Provided through the Hydrogen Station Cost Calculator

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen Infrastructure Market Readiness: Opportunities and Potential for Near-term Cost Reductions Proceedings of the Hydrogen Infrastructure Market Readiness Workshop and Summary of Feedback Provided through the Hydrogen Station Cost Calculator M.W. Melaina, D. Steward, and M. Penev National Renewable Energy Laboratory S. McQueen Energetics S. Jaffe and C. Talon IDC Energy Insights Technical Report NREL/BK-5600-55961 August 2012 NREL is a national laboratory of the U.S. Department of Energy,

  16. Refueling Infrastructure for Alternative Fuel Vehicles: Lessons Learned for Hydrogen; Workshop Proceedings

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Refueling Infrastructure for Alternative Fuel Vehicles: Lessons Learned for Hydrogen Workshop Proceedings M.W. Melaina National Renewable Energy Laboratory S. McQueen and J. Brinch Energetics Incorporated Sacramento, California April 3, 2008 Proceedings NREL/BK-560-43669 July 2008 NREL is operated by Midwest Research Institute ● Battelle Contract No. DE-AC36-99-GO10337 Refueling Infrastructure for Alternative Fuel Vehicles: Lessons Learned for Hydrogen Workshop Proceedings M.W. Melaina

  17. Hydrogen Infrastructure Market Readiness: Opportunities and Potential for Near-term Cost Reductions. Proceedings of the Hydrogen Infrastructure Market Readiness Workshop and Summary of Feedback Provided through the Hydrogen Station Cost Calculator

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Infrastructure Market Readiness: Opportunities and Potential for Near-term Cost Reductions Proceedings of the Hydrogen Infrastructure Market Readiness Workshop and Summary of Feedback Provided through the Hydrogen Station Cost Calculator M.W. Melaina, D. Steward, and M. Penev National Renewable Energy Laboratory S. McQueen Energetics S. Jaffe and C. Talon IDC Energy Insights Technical Report NREL/BK-5600-55961 August 2012 NREL is a national laboratory of the U.S. Department of Energy, Office of

  18. PROJECT PROFILE: Electric Power Research Institute (SHINES) | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy SHINES) PROJECT PROFILE: Electric Power Research Institute (SHINES) Title: Beneficial Integration of Energy Storage and Load Management with Photovoltaics epri-logo.jpg Funding Opportunity: Sustainable and Holistic Integration of Energy Storage and Solar PV SunShot Subprogram: Systems Integration Location: Knoxville, Tennessee Partners: FirstEnergy, NYPA, Con Edison, Southern Company, Gulf Power, Case Western Reserve University, Queens College of the City University of New York,

  19. John Ludlow

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    John Ludlow John Ludlow FES Requirements Worksheet 1.1. Project Information - Atomic and Molecular Physics for Controlled Fusion Energy Document Prepared By John Ludlow Project Title Atomic and Molecular Physics for Controlled Fusion Energy Principal Investigator John Ludlow Participating Organizations Auburn University Oak Ridge National Laboratory Los Alamos National Laboratory University of Strathclyde Queen's University of Belfast Funding Agencies DOE SC DOE NSA NSF NOAA NIH Other: EAEC,

  20. Gasoline and Diesel Fuel Update

    Gasoline and Diesel Fuel Update (EIA)

    Counties included in New York City metro area The list below includes the counties in the EIA-878 definition for New York City Metro Area. Bergen County, NJ Bronx County, NY Essex County, NJ Fairfield County, CT Hudson County, NJ Hunterdon County, NJ Kings County, NY Litchfield County (partial), CT Middlesex County, NJ Monmouth County, NJ Morris County, NJ Nassau County, NY New Haven County (partial), CT New York County, NY Ocean County, NJ Orange County, NY Passaic County, NJ Putnam, NY Queens

  1. Apartment Compartmentalization With an Aerosol-Based Sealing Process

    SciTech Connect (OSTI)

    Maxwell, Sean; Berger, David; Harrington, Curtis

    2015-03-01

    The U.S. Department of Energy Building America Team, Consortium for Advanced Residential Buildings, sought to demonstrate this new technology application in a new construction multifamily building in Queens, New York. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before-and-after sealing, point-source testing of individual leaks, and pressure measurements in the walls of an apartment during sealing.

  2. Identifying the ionically bound cell wall and intracellular glycoside hydrolases in late growth stage Arabidopsis stems: Implications for the genetic engineering of bioenergy crops

    SciTech Connect (OSTI)

    Wei, Hui; Brunecky, Roman; Donohoe, Bryon S.; Ding, Shi -You; Ciesielski, Peter N.; Yang, Shihui; Tucker, Melvin P.; Himmel, Michael E.

    2015-05-13

    Identifying the cell wall-ionically bound glycoside hydrolases (GHs) in Arabidopsis stems is important for understanding the regulation of cell wall integrity. For cell wall proteomics studies, the preparation of clean cell wall fractions is a challenge since cell walls constitute an open compartment, which is more likely to contain a mixture of intracellular and extracellular proteins due to cell leakage at the late growth stage. Here, for this study, we utilize a CaCl2-extraction procedure to isolate non-structural proteins from Arabidopsis whole stems, followed by the in-solution and in-gel digestion methods coupled with Nano-LC-MS/MS, bioinformatics and literature analyses. This has led to the identification of 75 proteins identified using the in-solution method and 236 proteins identified by the in-gel method, among which about 10% of proteins predicted to be secreted. Together, eight cell wall proteins, namely AT1G75040, AT5G26000, AT3G57260, AT4G21650, AT3G52960, AT3G49120, AT5G49360, and AT3G14067, were identified by the in-solution method; among them, three were the GHs (AT5G26000, myrosinase 1, GH1; AT3G57260, β-1,3-glucanase 2, GH17; AT5G49360, bifunctional XYL 1/α-L-arabinofuranosidase, GH3). Moreover, four more GHs: AT4G30270 (xyloglucan endotransferase, GH16), AT1G68560 (bifunctional α-l-arabinofuranosidase/XYL, GH31), AT1G12240 (invertase, GH32) and AT2G28470 (β-galactosidase 8, GH35), were identified by the in-gel solution method only. Notably, more than half of above identified GHs are xylan- or hemicellulose-modifying enzymes, and will likely have an impact on cellulose accessibility, which is a critical factor for downstream enzymatic hydrolysis of plant tissues for biofuels production. Finally, the implications of these cell wall proteins identified at the late growth stage for the genetic engineering of bioenergy crops are discussed.

  3. Identifying the ionically bound cell wall and intracellular glycoside hydrolases in late growth stage Arabidopsis stems: Implications for the genetic engineering of bioenergy crops

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wei, Hui; Brunecky, Roman; Donohoe, Bryon S.; Ding, Shi -You; Ciesielski, Peter N.; Yang, Shihui; Tucker, Melvin P.; Himmel, Michael E.

    2015-05-13

    Identifying the cell wall-ionically bound glycoside hydrolases (GHs) in Arabidopsis stems is important for understanding the regulation of cell wall integrity. For cell wall proteomics studies, the preparation of clean cell wall fractions is a challenge since cell walls constitute an open compartment, which is more likely to contain a mixture of intracellular and extracellular proteins due to cell leakage at the late growth stage. Here, for this study, we utilize a CaCl2-extraction procedure to isolate non-structural proteins from Arabidopsis whole stems, followed by the in-solution and in-gel digestion methods coupled with Nano-LC-MS/MS, bioinformatics and literature analyses. This has ledmore » to the identification of 75 proteins identified using the in-solution method and 236 proteins identified by the in-gel method, among which about 10% of proteins predicted to be secreted. Together, eight cell wall proteins, namely AT1G75040, AT5G26000, AT3G57260, AT4G21650, AT3G52960, AT3G49120, AT5G49360, and AT3G14067, were identified by the in-solution method; among them, three were the GHs (AT5G26000, myrosinase 1, GH1; AT3G57260, β-1,3-glucanase 2, GH17; AT5G49360, bifunctional XYL 1/α-L-arabinofuranosidase, GH3). Moreover, four more GHs: AT4G30270 (xyloglucan endotransferase, GH16), AT1G68560 (bifunctional α-l-arabinofuranosidase/XYL, GH31), AT1G12240 (invertase, GH32) and AT2G28470 (β-galactosidase 8, GH35), were identified by the in-gel solution method only. Notably, more than half of above identified GHs are xylan- or hemicellulose-modifying enzymes, and will likely have an impact on cellulose accessibility, which is a critical factor for downstream enzymatic hydrolysis of plant tissues for biofuels production. Finally, the implications of these cell wall proteins identified at the late growth stage for the genetic engineering of bioenergy crops are discussed.« less

  4. Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Inoue, Hiroyuki; Decker, Stephen R.; Taylor, Larry E.; Yano, Shinichi; Sawayama, Shigeki

    2014-10-09

    Background: Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide clues for maximizing the hydrolysis of target biomass. Talaromyces cellulolyticus is a promising fungus for cellulase production and efficient biomass hydrolysis. Several cellulolytic enzymes purified from T. cellulolyticus were characterized in earlier studies, but the core enzymes critical for hydrolysis of lignocellulosic biomass remain unknown. Results: Six cellulolytic enzymes critical for the hydrolysis of crystalline cellulose were purified from T. cellulolyticus culture supernatant using an enzyme assay based on synergistic hydrolysismore » of Avicel. The purified enzymes were identified by their substrate specificities and analyses of trypsin-digested peptide fragments and were classified into the following glycosyl hydrolase (GH) families: GH3 (β-glucosidase, Bgl3A), GH5 (endoglucanase, Cel5A), GH6 (cellobiohydrolase II, Cel6A), GH7 (cellobiohydrolase I and endoglucanase, Cel7A and Cel7B, respectively), and GH10 (xylanase, Xyl10A). Hydrolysis of dilute acid-pretreated corn stover (PCS) with mixtures of the purified enzymes showed that Cel5A, Cel7B, and Xyl10A each had synergistic effects with a mixture of Cel6A and Cel7A. Cel5A seemed to be more effective in the synergistic hydrolysis of the PCS than Cel7B. The ratio of Cel5A, Cel6A, Cel7A, and Xyl10A was statistically optimized for the hydrolysis of PCS glucan in the presence of Bgl3A. The resultant mixture achieved higher PCS glucan hydrolysis at lower enzyme loading than a culture filtrate from T. cellulolyticus or a commercial enzyme preparation, demonstrating that the five enzymes play a role as core enzymes in the hydrolysis of PCS glucan. In Conclusion: Core cellulolytic enzymes in the T. cellulolyticus cellulase system were identified to Cel5A, Cel6A, Cel7A, Xyl10A, and Bgl3A

  5. Identification and characterization of core cellulolytic enzymes from Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) critical for hydrolysis of lignocellulosic biomass

    SciTech Connect (OSTI)

    Inoue, Hiroyuki; Decker, Stephen R.; Taylor, Larry E.; Yano, Shinichi; Sawayama, Shigeki

    2014-10-09

    Background: Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide clues for maximizing the hydrolysis of target biomass. Talaromyces cellulolyticus is a promising fungus for cellulase production and efficient biomass hydrolysis. Several cellulolytic enzymes purified from T. cellulolyticus were characterized in earlier studies, but the core enzymes critical for hydrolysis of lignocellulosic biomass remain unknown. Results: Six cellulolytic enzymes critical for the hydrolysis of crystalline cellulose were purified from T. cellulolyticus culture supernatant using an enzyme assay based on synergistic hydrolysis of Avicel. The purified enzymes were identified by their substrate specificities and analyses of trypsin-digested peptide fragments and were classified into the following glycosyl hydrolase (GH) families: GH3 (β-glucosidase, Bgl3A), GH5 (endoglucanase, Cel5A), GH6 (cellobiohydrolase II, Cel6A), GH7 (cellobiohydrolase I and endoglucanase, Cel7A and Cel7B, respectively), and GH10 (xylanase, Xyl10A). Hydrolysis of dilute acid-pretreated corn stover (PCS) with mixtures of the purified enzymes showed that Cel5A, Cel7B, and Xyl10A each had synergistic effects with a mixture of Cel6A and Cel7A. Cel5A seemed to be more effective in the synergistic hydrolysis of the PCS than Cel7B. The ratio of Cel5A, Cel6A, Cel7A, and Xyl10A was statistically optimized for the hydrolysis of PCS glucan in the presence of Bgl3A. The resultant mixture achieved higher PCS glucan hydrolysis at lower enzyme loading than a culture filtrate from T. cellulolyticus or a commercial enzyme preparation, demonstrating that the five enzymes play a role as core enzymes in the hydrolysis of PCS glucan. In Conclusion: Core cellulolytic enzymes in the T. cellulolyticus cellulase system were identified to Cel5A, Cel6A, Cel7A, Xyl10A, and Bgl3A and

  6. Pressurized subsampling system for pressured gas-hydrate-bearing sediment: Microscale imaging using X-ray computed tomography

    SciTech Connect (OSTI)

    Jin, Yusuke Konno, Yoshihiro; Nagao, Jiro

    2014-09-01

    A pressurized subsampling system was developed for pressured gas hydrate (GH)-bearing sediments, which have been stored under pressure. The system subsamples small amounts of GH sediments from cores (approximately 50 mm in diameter and 300 mm in height) without pressure release to atmospheric conditions. The maximum size of the subsamples is 12.5 mm in diameter and 20 mm in height. Moreover, our system transfers the subsample into a pressure vessel, and seals the pressure vessel by screwing in a plug under hydraulic pressure conditions. In this study, we demonstrated pressurized subsampling from artificial xenon-hydrate sediments and nondestructive microscale imaging of the subsample, using a microfocus X-ray computed tomography (CT) system. In addition, we estimated porosity and hydrate saturation from two-dimensional X-ray CT images of the subsamples.

  7. S U M M A R I E S U.S. Energy Information Administration | State Energy Data 2014: Prices and Expenditures

    Gasoline and Diesel Fuel Update (EIA)

    1 Table E9. Total End-Use Energy Expenditure Estimates, 2014 (Million Dollars) State Primary Energy Retail Electricity Total Energy g,h Coal Natural Gas a Petroleum Biomass Total g,h Distillate Fuel Oil Jet Fuel b LPG c Motor Gasoline d Residual Fuel Oil Other e Total Wood and Waste f Alabama 360.8 1,880.9 3,696.9 283.1 226.6 8,039.9 97.2 713.3 13,057.0 484.4 15,783.2 8,363.3 24,146.5 Alaska 40.2 319.2 2,020.9 2,013.3 32.9 1,125.1 - 263.1 5,455.4 20.0 5,834.8 1,055.8 6,890.6 Arizona 15.3 1,065.5

  8. S U M M A R I E S U.S. Energy Information Administration | State Energy Data 2014: Prices and Expenditures

    Gasoline and Diesel Fuel Update (EIA)

    4 Table E2. Total End-Use Energy Price Estimates, 2014 (Dollars per Million Btu) State Primary Energy Retail Electricity Total Energy g,h Coal Natural Gas a Petroleum Biomass Total g,h Distillate Fuel Oil Jet Fuel b LPG c Motor Gasoline d Residual Fuel Oil Other e Total Wood and Waste f Alabama 4.13 7.48 25.92 19.92 26.79 25.88 12.59 28.08 25.65 3.46 15.98 27.20 18.64 Alaska 4.83 8.66 28.76 20.97 27.65 33.67 - 19.35 25.44 10.91 22.31 51.27 24.43 Arizona 2.94 11.85 26.90 21.30 30.28 27.09 - 23.91

  9. H2A Delivery: Forecourt Compression & Storage Optimization (Part II) |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Delivery: Forecourt Compression & Storage Optimization (Part II) H2A Delivery: Forecourt Compression & Storage Optimization (Part II) Presentation by Matthew Hooks of TIAX at the Joint Meeting on Hydrogen Delivery Modeling and Analysis, May 8-9, 2007 deliv_analysis_hooks.pdf (482.38 KB) More Documents & Publications H2A Delivery: GH2 and LH2 Forecourt Land Areas Forecourt Storage and Compression Options

  10. Questions and Issues on Hydrogen Pipelines: Pipeline Transmission of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hydrogen | Department of Energy Questions and Issues on Hydrogen Pipelines: Pipeline Transmission of Hydrogen Questions and Issues on Hydrogen Pipelines: Pipeline Transmission of Hydrogen Pipping of GH2 Pipeline. Background: FG 64 built in 50ies, KP added in 70ies, active mining area over total length hpwgw_questissues_campbell.pdf (1.02 MB) More Documents & Publications Blending Hydrogen into Natural Gas Pipeline Networks: A Review of Key Issues Hydrogen Pipeline Discussion EIS-0487:

  11. Liquid scintillators for optical fiber applications

    DOE Patents [OSTI]

    Franks, Larry A.; Lutz, Stephen S.

    1982-01-01

    A multicomponent liquid scintillator solution for use as a radiation-to-light converter in conjunction with a fiber optic transmission system. The scintillator includes a quantity of 1, 2, 4, 5, 3H, 6H, 1 OH, tetrahydro-8-trifluoromethyl (1) benzopyrano (9, 9a, 1-gh) quinolizin-10-one (Coumarin) as a solute in a fluor solvent such as benzyl alcohol or pseudo-cumene. The use of BIBUQ as an additional or primary solute is also disclosed.

  12. Chronic treatment with polychlorinated biphenyls (PCB) during pregnancy and lactation in the rat

    SciTech Connect (OSTI)

    Cocchi, Daniela; Tulipano, Giovanni; Colciago, Alessandra; Sibilia, Valeria; Pagani, Francesca; Vigano, Daniela; Rubino, Tiziana; Parolaro, Daniela; Bonfanti, Patrizia; Colombo, Anita; Celotti, Fabio

    2009-06-01

    Polychlorinated biphenyls (PCBs) are pollutants detected in animal tissues and breast milk. The experiments described in the present paper were aimed at evaluating whether the four PCB congeners most abundant in animal tissues (PCB-138, -153, -180 and -126), administered since fetal life till weaning, can induce long-term alterations of GH-axis activity and bone mass in the adult rat. We measured PCB accumulation in rat brain and liver, somatic growth, pituitary GH expression and plasma hormone concentrations at different ages. Finally, we studied hypothalamic somatostatin expression and bone structure in adulthood, following long-term PCB exposure. Dams were treated during pregnancy from GD15 to GD19 and during breast-feeding. A constant reduction of the growth rate in both male and female offspring from weaning to adulthood was observed in exposed animals. Long-lasting alterations on hypothalamic-pituitary GH axis were indeed observed in PCB-exposed rats in adulthood: increased somatostatin expression in hypothalamic periventricular nucleus (both males and females) and lateral arcuate nucleus (males, only) and decreased GH mRNA levels in the pituitary of male rats. Plasma IGF-1 levels were higher in PCB-exposed male and female animals as compared with controls at weaning and tended to be higher at PN60. Plasma testosterone and thyroid hormone concentrations were not significantly affected by exposure to PCBs. In adulthood, PCBs caused a significant reduction of bone mineral content and cortical bone thickness of tibiae in male rat joint to increased width of the epiphyseal cartilage disk. In conclusion, the developmental exposure to the four selected PCB compounds used in the present study induced far-reaching effects in the adult offspring, the male rats appearing more sensitive than females.

  13. Fourth DOE Natural Phenomena Hazards Mitigation Conference: Proceedings. Volume 1

    SciTech Connect (OSTI)

    Not Available

    1993-12-31

    This conference allowed an interchange in the natural phenomena area among designers, safety professionals, and managers. The papers presented in Volume I of the proceedings are from sessions I - VIII which cover the general topics of: DOE standards, lessons learned and walkdowns, wind, waste tanks, ground motion, testing and materials, probabilistic seismic hazards, risk assessment, base isolation and energy dissipation, and lifelines and floods. Individual papers are indexed separately. (GH)

  14. High frequency background quantization of gravity

    SciTech Connect (OSTI)

    von Borzeszkowski, H.

    1982-06-01

    Considering background quantization of gravitational fields, it is generally assumed that the classical background satisfies Einstein's gravitational equations. However, there exist arguments showing that, for high frequency (quantum) fluctuations, this assumption has to be replaced by a condition describing the back reaction of fluctuations on the background. It is shown that such an approach leads to limitations for the quantum procedure which occur at distances larger than Planck's elementary length 1 = (Gh/c/sup 3/)/sup 1/2/.

  15. Coupling of Mechanical Behavior of Cell Components to Electrochemical-Thermal Models for Computer- Aided Engineering of Batteries under Abuse

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Coupling of Mechanical Behavior of Cell Components to Electrochemical-Thermal Models for Computer- Aided Engineering of Batteries under Abuse P.I.: Ahmad Pesaran Team: Tomasz Wierzbicki and Elham Sahraei (MIT) Genong Li and Lewis Collins (ANSYS) M. Sprague, G.H. Kim and S. Santhangopalan (NREL) June 17, 2014 This presentation does not contain any proprietary, confidential, or otherwise restricted information. Project ID: ES199 NREL/PR-5400-61885 2 Overview * Project Start: October 2013 * Project

  16. Differential Recognition and Hydrolysis of Host Carbohydrate Antigens by Streptococcus pneumoniae Family 98 Glycoside Hydrolases

    SciTech Connect (OSTI)

    Higgins, M.; Whitworth, G; El Warry, N; Randriantsoa, M; Samain, E; Burke, R; Vocadlo, D; Boraston, A

    2009-01-01

    The presence of a fucose utilization operon in the Streptococcus pneumoniae genome and its established importance in virulence indicates a reliance of this bacterium on the harvesting of host fucose-containing glycans. The identities of these glycans, however, and how they are harvested is presently unknown. The biochemical and high resolution x-ray crystallographic analysis of two family 98 glycoside hydrolases (GH98s) from distinctive forms of the fucose utilization operon that originate from different S. pneumoniae strains reveal that one enzyme, the predominant type among pneumococcal isolates, has a unique endo-{beta}-galactosidase activity on the LewisY antigen. Altered active site topography in the other species of GH98 enzyme tune its endo-{beta}-galactosidase activity to the blood group A and B antigens. Despite their different specificities, these enzymes, and by extension all family 98 glycoside hydrolases, use an inverting catalytic mechanism. Many bacterial and viral pathogens exploit host carbohydrate antigens for adherence as a precursor to colonization or infection. However, this is the first evidence of bacterial endoglycosidase enzymes that are known to play a role in virulence and are specific for distinct host carbohydrate antigens. The strain-specific distribution of two distinct types of GH98 enzymes further suggests that S. pneumoniae strains may specialize to exploit host-specific antigens that vary from host to host, a factor that may feature in whether a strain is capable of colonizing a host or establishing an invasive infection.

  17. TRUPACT-II Hydrogen G-Valve Program Test Plan

    SciTech Connect (OSTI)

    Mroz, Eugene J.

    1999-01-01

    This test plan describes the objectives, scope, participants, and components of the Transuranic Package Transporter-II (TRUPACT-II) Hydrogen G-Value Program (GH2P). The GH2P builds on the experience, results, and experimental setup of the TRUPACT-II Matrix Depletion Program (MDP) to establish effective hydrogen G-values (G-values) for additional waste matrices. This plan details the experimental design and test matrices for experiments to measure the G-value for additional waste matrices, including first- and second-stage sludges at the Idaho National Engineering and Environmental Laboratory, and molten salt extraction residues with varying amounts of residual moisture (i.e., unbound water). Data collected from the GH2P will be used to support an application to the US Nuclear Regulatory Commission for G-values and corresponding wattage limits for the TRUPACT-II payloads containing these waste matrices. The testing will also evaluate the ability to determine G-values on a waste stream basis.

  18. Spin and valley transport in monolayers of MoS{sub 2}

    SciTech Connect (OSTI)

    Sun, J. F.; Cheng, F.

    2014-04-07

    We investigate theoretically quantum transport and Goos-Hnchen (GH) effect of electrons in a p-n-p junction on monolayers of MoS{sub 2}. We find that the transmission properties of spin-up (spin-down) electrons in K valley are the same with spin-down (spin-up) electrons in K? valley due to the time-reversal symmetry. The GH shifts for the transmitted K and K? beams in the n-p interface are in the opposite direction, and GH shifts for the spin-up and spin-down electron beams at the same valley have different values in the same direction due to the different group velocities. Therefore, the spin-up and spin-down electrons can be separated after passing a sufficiently long channel created by a p-n-p junction. These features provide us a new way to generate a fully spin- and valley-polarized current in monolayers of MoS{sub 2}.

  19. Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-β-d-xylanase 10B in complex with xylohexaose

    SciTech Connect (OSTI)

    Najmudin, Shabir; Pinheiro, Benedita A.; Romão, Maria J.; Prates, José A. M.; Fontes, Carlos M. G. A.

    2008-08-01

    The N-terminal moiety of C. thermocellum endo-1,4-β-d-xylanase 10B, comprising a carbohydrate-binding module (CBM22-1) and a GH10 E337A mutant domain, has been crystallized in complex with xylohexaose. The crystals belong to the trigonal space group P3{sub 2}21, contain a dimer in the asymmetric unit and diffract to beyond 2.0 Å resolution. The cellulosome of Clostridium thermocellum is a highly organized multi-enzyme complex of cellulases and hemicellulases involved in the hydrolysis of plant cell-wall polysaccharides. The bifunctional multi-modular xylanase Xyn10B is one of the hemicellulase components of the C. thermocellum cellulosome. The enzyme contains an internal glycoside hydrolase family 10 catalytic domain (GH10) and a C-terminal family 1 carbohydrate esterase domain (CE1). The N-terminal moiety of Xyn10B (residues 32–551), comprising a carbohydrate-binding module (CBM22-1) and the GH10 E337A mutant, was crystallized in complex with xylohexaose. The crystals belong to the trigonal space group P3{sub 2}21 and contain a dimer in the asymmetric unit. The crystals diffracted to beyond 2.0 Å resolution.

  20. Effects of high-voltage transmission lines on honeybees

    SciTech Connect (OSTI)

    Greenberg, B.; Bindokas, V.P.; Gauger, J.R.

    1985-05-01

    When shielded and exposed colonies were placed at incremental distances at a right angle from a 760-kV transmission line different thresholds for biologic effects were obtained. Hive exposures were controlled (E-field: 7, 5.5, 4.1, 1.8, and 0.65 to 0.85 kV/m) by variable height current collectors; shielded hives under the line behave normally. Exposure to 7 kV/m can produce the following sequence of events: (1) increased motor activity and transient hive temperature increase; (2) abnormal propolization; (3) retarded hive weight gain; (4) excess queen cell production with queen loss; (5) reduction of sealed brood area; and (6) poor winter survival. No biological effects were detected below 4.1 kV/m, thus the ''biological effects corridor'' is limited to approximately 23 m beyond a ground projection of each outer phase wire. Hive architecture enhances E-fields and creates shock hazards for bees. Intra-hive E-fields (15 to 100+ kV/m) were measured with a displacement current sensor and fiber optic telemetry link. Step-potential-induced currents up to 0.5 uA were measured with a bee model in hives at 7 kV/m. To investigate further the role of shock versus electric field exposure the study was continued to develop hive entrance extensions (porches), which produce controlled bee exposure to E-field or shock, and to test the feasibility of using these porches in such a study. Biological effects (e.g., abnormal propolization, retarded hive weight, queen loss) found in colonies with total-hive exposure were produced by entrance-only exposure of adult bees. We now have an exposure system in which E-field and shock can be separately controlled to reproduce the biological effects. 10 refs.

  1. Approximate resolution of hard numbering problems

    SciTech Connect (OSTI)

    Bailleux, O.; Chabrier, J.J.

    1996-12-31

    We present a new method for estimating the number of solutions of constraint satisfaction problems. We use a stochastic forward checking algorithm for drawing a sample of paths from a search tree. With this sample, we compute two values related to the number of solutions of a CSP instance. First, an unbiased estimate, second, a lower bound with an arbitrary low error probability. We will describe applications to the Boolean Satisfiability problem and the Queens problem. We shall give some experimental results for these problems.

  2. Novel Material for Efficient and Low-cost Separation of Gases for Fuels and Plastics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Material for Efficient and Low-Cost Separation of Gases for Fuels and Plastics Work was performed at University of California and supported by the Center for Gas Separations Relevant to Clean Energy Technologies EFRC. Bloch, E. D.; Queen, W. L.; Krishna, R.; Zadrozny, J. M.; Brown, C. M.; Long, J. R. Science 2012, 335, 1606-1610 Left: Crystal structure of Fe 2 (dobdc)-ethylene showing Fe (orange), O(red), C(gray), and D(blue) atoms. The view along the [001] direction shows an ethylene molecule

  3. Slide 1

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bloch, E. D.; Hudson, M. R.; Mason, J. A.; Chavan, S.; Crocellà, V.; Howe, J. D.; Lee, K.; Dzubak, A. L.; Queen, W. L.; Zadrozny, J. M.; Geier, S. J.; Lin, L.- C.; Gagliardi, L.; Smit, B.; Neaton, J. B.; Bordiga, S.; Brown, C. M.; Long, J. R. J. Am. Chem. Soc. 2014, 136, 10752-10761. Reversible CO Binding in Metal-Organic Frameworks Scientific Achievement Six metal-organic frameworks with different exposed divalent metal cations can bind CO reversibly and at high capacity Significance and

  4. Mailing Addresses for National Laboratories and Technology Centers |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Mailing Addresses for National Laboratories and Technology Centers Mailing Addresses for National Laboratories and Technology Centers Name Telephone Number U.S. Department of Energy Albany Research Center 1450 Queen Ave. SW Albany, OR 97321-2198 541-967-5892 U.S. Department of Energy Ames Laboratory #311 TASF, Iowa State University Ames, Iowa 50011 515-294-2680 U.S. Department of Energy Argonne National Laboratory (East) 9700 S. Cass Avenue Argonne, IL 60439 630-252-2000

  5. COLLOQUIUM: NOTE SPECIAL DATE - THURSDAY: Unique Vulnerability of the New

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    York/New Jersey Metro Region to Hurricane Destruction - A New Perspective Based on Recent Research on Irene 2011 and Sandy 2012 | Princeton Plasma Physics Lab February 28, 2013, 4:15pm to 5:30pm Colloquia MBG Auditorium COLLOQUIUM: NOTE SPECIAL DATE - THURSDAY: Unique Vulnerability of the New York/New Jersey Metro Region to Hurricane Destruction - A New Perspective Based on Recent Research on Irene 2011 and Sandy 2012 Professor Nicholas K. Coch Queens College CUNY In the last two years. the

  6. Women @ Energy: Cindy Joe | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Women @ Energy: Christina Swinson Christina Swinson is a post doc. in Accelerator Physics at Brookhaven National Lab. She has a BSc. In Physics with Computing, Queen Mary College, University of London, United Kingdom, and a DPhil. (PhD.) in Accelerator Physics from the University of Oxford, United Kingtom. 1) What inspired you to work in STEM? My decision to work in STEM didn't come until I was in my twenties but the journey first began in high school. Unfortunately at the time I wasn't

  7. Pallet insertion glovebox/hood control ladder diagram. Final project report

    SciTech Connect (OSTI)

    Issaian, V.

    1995-12-01

    The pallet insertion glovebox/hood (G/H) is a special confinement space that will be designed to allow for insertion of pallets into the Stacker/Retriever (S/R) area. The S/R a large vault that is kept at negative 1 inches w.c. relative to the atmosphere and is used for the safe storage of special nuclear material. The S/R system uses a vehicle to move the special nuclear material that are placed on the pallets from the storage bins to input/output (I/O) stations and vice versa. As the name suggest the I/O stations are used to place the material into the S/R vault or to remove material from the S/R vault. The pallets are specially designed structures that will hold certain numbers of the material containers in a safe configuration. To store additional material containers, there is a need to insert additional pallets in the SIR vault. Due to the presence of radioactive contamination and the fact that the vault must be kept at a negative pressure at all times, one of the several I/O stations will be modified so that pallets could be inserted into the S/R vault. The ventilation system for the S/R area is a dedicated system that recirculates nitrogen with less than 5% oxygen by volume throughout the area while exhausting small option of the nitrogen to keep the S/R at negative 1 inches w.c. relative to the atmosphere. The rooms surrounding the G/H and the S/R area are maintained at negative of 0.3 inches w.c. relative to the outside atmosphere. Both the G/H and the control system for the G/H will be designed such that the confinement requirements of the S/R and the G/H system will not be jeopardized. A ladder diagram will be developed to illustrate the control system.

  8. Biochemical and structural characterizations of two Dictyostelium cellobiohydrolases from the amoebozoa kingdom reveal a high level of conservation between distant phylogenetic trees of life

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hobdey, Sarah E.; Knott, Brandon C.; Momeni, Majid Haddad; Taylor, II, Larry E.; Borisova, Anna S.; Podkaminer, Kara K.; VanderWall, Todd A.; Himmel, Michael E.; Decker, Stephen R.; Beckham, Gregg T.; et al

    2016-04-01

    Glycoside hydrolase family 7 (GH7) cellobiohydrolases (CBHs) are enzymes often employed in plant cell wall degradation across eukaryotic kingdoms of life, as they provide significant hydrolytic potential in cellulose turnover. To date, many fungal GH7 CBHs have been examined, yet many questions regarding structure-activity relationships in these important natural and commercial enzymes remain. Here, we present the crystal structures and a biochemical analysis of two GH7 CBHs from social amoeba: Dictyostelium discoideum Cel7A (DdiCel7A) and Dictyostelium purpureum Cel7A (DpuCel7A). DdiCel7A and DpuCel7A natively consist of a catalytic domain and do not exhibit a carbohydrate-binding module (CBM). The structures of DdiCel7Amore » and DpuCel7A, resolved to 2.1 Å and 2.7 Å, respectively, are homologous to those of other GH7 CBHs with an enclosed active-site tunnel. Two primary differences between the Dictyostelium CBHs and the archetypal model GH7 CBH, Trichoderma reesei Cel7A (TreCel7A), occur near the hydrolytic active site and the product-binding sites. To compare the activities of these enzymes with the activity of TreCel7A, the family 1 TreCel7A CBM and linker were added to the C terminus of each of the Dictyostelium enzymes, creating DdiCel7ACBM and DpuCel7ACBM, which were recombinantly expressed in T. reesei. DdiCel7ACBM and DpuCel7ACBM hydrolyzed Avicel, pretreated corn stover, and phosphoric acid-swollen cellulose as efficiently as TreCel7A when hydrolysis was compared at their temperature optima. The Ki of cellobiose was significantly higher for DdiCel7ACBM and DpuCel7ACBM than for TreCel7A: 205, 130, and 29 μM, respectively. Finally, taken together, the present study highlights the remarkable degree of conservation of the activity of these key natural and industrial enzymes across quite distant phylogenetic trees of life.« less

  9. Biochemical and Structural Characterization of Two Dictyostelium Cellobiohydrolases from the Amoebozoa Kingdom Reveal a High Level of Conservation Between Distant Phylogenetic Trees of Life

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hobdey, Sarah E.; Knott, Brandon C.; Momeni, Majid Haddad; Taylor, II, Larry E.; Borisova, Anna S.; Podkaminer, Kara K.; VanderWall, Todd A.; Himmel, Michael E.; Decker, Stephen R.; Beckham, Gregg T.; et al

    2016-06-01

    Glycoside Hydrolase Family 7 (GH7) cellobiohydrolases (CBHs) are commonly employed enzymes in plant cell wall degradation across eukaryotic kingdoms of life, as they provide significant hydrolytic potential in cellulose turnover. To date, many fungal GH7 CBHs have been examined, yet many questions remain regarding structure-activity relationships in these important natural and commercial enzymes. Here, we present crystal structures and biochemical analysis of two GH7 CBHs from social amoeba: Dictyostelium discoideum and Dictyostelium purpureum (DdiCel7A and DpuCel7A, respectively). DdiCel7A and DpuCel7A natively consist of a catalytic domain and do not exhibit a carbohydrate-binding module (CBM). The structures, resolved to 2.1 Amore » (DdiCel7A), and 2.7 A (DpuCel7A), are homologous to other GH7 CBHs with an enclosed active site tunnel. Two primary differences between the Dictyostelium CBHs and the archetypal model GH7 CBH from Trichoderma reesei Cel7A (TreCel7A) occur near the hydrolytic active site and the product binding sites. To compare the activity of these enzymes with TreCel7A, the Family 1 TreCel7A CBM and linker was added to the C-terminus of the Dictyostelium enzymes, DdiCel7ACBM and DpuCel7ACBM, which were recombinantly expressed in T. reesei. DdiCel7ACBM and DpuCel7ACBM hydrolyze Avicel, pretreated corn stover, and phosphoric acid swollen cellulose as efficiently as TreCel7A when compared at their temperature optima. The Ki of cellobiose is significantly higher for DdiCel7ACBM and DpuCel7ACBM than for TreCel7A: 205, 130, and 29 uM, respectively. Taken together, the present study highlights the remarkable conservation in the activity of these key natural and industrial enzymes across quite distant phylogenetic trees of life.« less

  10. Electronics for the Si detectors in APEX

    SciTech Connect (OSTI)

    Wilt, P.R. |; Betts, R.R.; Freer, M.

    1994-07-01

    APEX (ATLAS Positron EXperiment), a collaborative effort of ANL, FSU, MSU/NSCL, Princeton, Queen`s, Rochester, Washington and Yale, is an experiment to study positron and electron production in very heavy ion collisions. The electrons and positrons are detected with two detector arrays, each consisting of 216 1 mm thick Si PIN diodes, and their energy and time-of-flight are measured. The number of detectors and limited space made it necessary to develop a system that could efficiently process and transfer signals from the detectors to the charge sensing ADC`s and data readout electronics as well as monitor the condition of the detectors. The discussion will cover the electronics designed for the Si detectors, including the charge amplifier, ``Mother board`` for the charge amplifiers, 8 channel Shaper, 16 channel Constant Fraction Discriminator (CFD), 16 channel Peak-to-FERA (PTF) and the integration of the CFD and PTF with Charge sensing ADC`s. Function and performance of the individual modules as well as the system as a whole will be discussed.

  11. Integration of advanced geoscience and engineering techniques to quantify inter-well heterogeneity. Quarterly technical report, January 1--March 31, 1994

    SciTech Connect (OSTI)

    Buckley, J.S.; Weiss, W.W.; Ouenes, A.

    1994-06-01

    The purpose of this project is to conduct a variety of laboratory and field tests and utilize all the geological, geophysical, and engineering information collected to develop a reservoir model by the use of global optimization methods. The interdisciple effort will integrate advanced geoscience and reservoir engineering concepts to quantify reservoir heterogeneity and the dynamics of fluid-rock and fluid-fluid interactions. The reservoir characterization will include geological methods (outcrop and reservoir rock studies), geophysical methods (interwell acoustic techniques), and other reservoir/hydrologic methodologies including analyses of pressure transient data, field tracer tests, and laboratory core studies. The field testing will be conducted at the Sulimar Queen Unit with related laboratory testing at the Petroleum Recovery Research Center (PRRC) on core samples from the Sulimar site and Queen sandstone outcrops. Research methods will involve the acquisition of data obtained at different scales and integration of the data into a reservoir model. The goals of the project are to: (1) characterize lithologic heterogeneity, (2) quantify changes in heterogeneity at various scales, (3) integrate the wide variety of data into a model that is jointly constrained by the interdisciplinary interpretive effort, and (4) achieve greater accuracy and confidence during simulation and modeling as steps toward optimizing recovery efficiency from existing petroleum reservoirs.

  12. Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Young, Jenna; Chung, Daehwan; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2014-10-09

    Background: Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. Different species vary in their abilities to degrade cellulose, and the presence of CelA, a bifunctional glycoside hydrolase that contains a Family 48 and a Family 9 catalytic domain, correlates well with cellulolytic ability in members of this genus. For example, C. hydrothermalis, which does not contain a CelA homolog, or a GH48 Family or GH9 Family glycoside hydrolase, is the least cellulolytic of the Caldicellulosiruptor species so far described. C. bescii,more » which contains CelA and expresses it constitutively, is among the most cellulolytic. In fact, CelA is the most abundant extracellular protein produced in C. bescii. The enzyme contains two catalytic units, a Family 9A-CBM3c processive endoglucanase and a Family 48 exoglucanase, joined by two Family 3b carbohydrate-binding domains. Although there are two non-reducing end-specific Family 9 and three reducing end-specific Family 48 glycoside hydrolases (producing primarily glucose and cellobiose; and cellobiose and cellotriose, respectively) in C. bescii, CelA is the only protein that combines both enzymatic activities. Results: A deletion of the celA gene resulted in a dramatic reduction in the microorganism’s ability to grow on crystalline cellulose (Avicel) and diminished growth on lignocellulosic biomass. A comparison of the overall endoglucanase and exoglucanase activities of the mutant compared with the wild-type suggests that the loss of the endoglucanase activity provided by the GH9 family domain is perhaps compensated for by other enzymes produced by the cell. In contrast, it appears that no other enzymes in the C. bescii secretome can compensate for the loss of exoglucanase activity. The change in enzymatic activity in the celA mutant resulted in a 15-fold decrease in

  13. Fuel Cell Technologies Program - DOD-DOE Workshop: Shipboard APUs Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    FUEL CELL TECHNOLOGIES PROGRAM DOD-DOE Workshop: Shipboard APUs Overview 3/29/2011 Dr. Sunita Satyapal Program Manager F l C ll T h l i P Fuel Cell Technologies Program U.S. Department of Energy Rest of E U France 272 MW Germany 9 785 MW U.S. Rest of E.U. 1,333 MW 9,785 MW Japan U.S. share of PV production has fallen significantly over the last 10 years Global & U.S. Annual PV Production by Region Cumulative Installed PV ( (throug gh 2009) ) Italy 1,167 MW China 305 MW 1,650 MW Rest of

  14. U.S. Energy Information Administration (EIA)

    U.S. Energy Information Administration (EIA) Indexed Site

    6 State Heating Oil and Propane Program (SHOPP) Workshop U.S. Department of Energy Forrestal Building 1000 Independence Avenue SW Washington, DC 20585 Room GH-027 Wednesday, July 13, 2016 9:00 am - 3:15 pm Time Workshop agenda 8:00 - 8:45 am U.S. Department of Energy Visitor Registration** Please see required forms of identification 8:30 - 9:15 am Light breakfast and coffee 9:15 - 9:20 am Welcome - Adam Sieminski, Administrator, U.S. Energy Information Administration 9:20 - 9:45 am Biofuel

  15. Co-Optimization of Fuels and Engines (Co-Optima) -- Fuel Properties and Chemical Kinetics and Thrust I Engine Projects

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    This presentation does not contain any proprietary, confidential, or otherwise restricted information #$%&'"(")*+,"!" Co-Optimization of Fuels and Engines Fuel Property Team -./0+,"12"345.+67489 ! "!"#$%&"'('#") * %+",%-./0"12) 3 %4",%5$/1) 6 %7$89%:"9;1) <% :$=%:>?;#9/) @ %5"99%7"2.) @ %+'#%A8;>B;) * %:$=%C$2>9"D) * %5($E%F"G9;() * %->'=%!'9E10'('8GH I % *J!

  16. Co-Optimization of Fuels and Engines (Co-Optima) --Simulation Toolkit Team

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Co-Optimization of Fuels and Engines (Co-Optima) - Simulation Toolkit Team This presentation does not contain any proprietary, confidential, or otherwise restricted information #$%&%" '("')*+,-./ ! "0("012/ 3 "!"#$%&&'()*+(, - #"."#!"#/01%&02, 3# 4"#5&+6*, 7# 8"#.+0%9%2%:, ;# 5"#<%=%>?, @# 8"# A?B?:?'(, @ #C"#C%:, ; #D"#4%E, ; #F"#D%(#!%E, ; #8"#G%*?&2, - #%(0#4"#GH'*?2'0?2 I#

  17. Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass

    SciTech Connect (OSTI)

    Young, Jenna; Chung, Daehwan; Bomble, Yannick J.; Himmel, Michael E.; Westpheling, Janet

    2014-10-09

    Background: Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. Different species vary in their abilities to degrade cellulose, and the presence of CelA, a bifunctional glycoside hydrolase that contains a Family 48 and a Family 9 catalytic domain, correlates well with cellulolytic ability in members of this genus. For example, C. hydrothermalis, which does not contain a CelA homolog, or a GH48 Family or GH9 Family glycoside hydrolase, is the least cellulolytic of the Caldicellulosiruptor species so far described. C. bescii, which contains CelA and expresses it constitutively, is among the most cellulolytic. In fact, CelA is the most abundant extracellular protein produced in C. bescii. The enzyme contains two catalytic units, a Family 9A-CBM3c processive endoglucanase and a Family 48 exoglucanase, joined by two Family 3b carbohydrate-binding domains. Although there are two non-reducing end-specific Family 9 and three reducing end-specific Family 48 glycoside hydrolases (producing primarily glucose and cellobiose; and cellobiose and cellotriose, respectively) in C. bescii, CelA is the only protein that combines both enzymatic activities. Results: A deletion of the celA gene resulted in a dramatic reduction in the microorganism’s ability to grow on crystalline cellulose (Avicel) and diminished growth on lignocellulosic biomass. A comparison of the overall endoglucanase and exoglucanase activities of the mutant compared with the wild-type suggests that the loss of the endoglucanase activity provided by the GH9 family domain is perhaps compensated for by other enzymes produced by the cell. In contrast, it appears that no other enzymes in the C. bescii secretome can compensate for the loss of exoglucanase activity. The change in enzymatic activity in the celA mutant resulted in a 15-fold decrease in sugar

  18. Publications

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Fundamental Mechanisms of Transient States in Materials Quantified by DTEM Recent Publications N.D. Browning, M.A. Bonds, G.H. Campbell, J.E. Evans, T. LaGrange, K.L. Jungjohann, D.J. Masiel, J. McKeown, S. Mehraen, B.W. Reed, M. Santala, "Recent Developments in Dynamic Transmission Electron Microscopy," Current Opinion in Solid State and Materials Science, 16 [1] 23 - 30 (2012). Thomas LaGrange, Bryan W. Reed, Melissa Santala, Joseph McKeown, Andreas Kulovits, Jörg M. K. Wiezorek,

  19. I

    Office of Legacy Management (LM)

    - . 'i,. . 4.... J ' I fice Memorandum * UNITED STATES GOVERNMENT ( :{:o ,: J.K. Gust&sfon, Director, Division of Raw DATE: August 4, 1948 P/ Materials I:OM :F.. SJr, and M.G. McGrath t SU3JECTSOIC2Gh:G TI£'N OF CAO30TITE ORES BY VANADIUM CORPORATICN OF AMT.ICA Tihe modern accepted laboratory research practice to develop the lowest cost and most eff.icient method of mineral dressing is, first, to attempt ccncen- tration of the ore minerals, with subsequent treatment of the concent,-ted

  20. Laser Crystallization of Phase Change Material

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Geoffrey Campbell is the Principal Investigator for Laser Crystallization of Phase Change Material LLNL BES Programs Highlight Laser Crystallization of Phase Change Material False color images of growth of crystalline GeTe (yellow) into amorphous GeTe (blue) in a series of 17.5 ns images during crystallization induced by a 12 ns laser pulse M.K. Santala, B.W. Reed, S. Raoux, T. Topuria, T. LaGrange, and G.H. Campbell, "Irreversible Reactions Studied with Nanosecond TEM Movies: Laser

  1. DOE/EIA-0376(2014)

    Gasoline and Diesel Fuel Update (EIA)

    376(2014) June 2016 State Energy Price and Expenditure Estimates 1970 Through 2014 2014 Price and Expenditure Summary Tables S U M M A R I E S U.S. Energy Information Administration | State Energy Data 2014: Prices and Expenditures 3 Table E1. Primary Energy, Electricity, and Total Energy Price Estimates, 2014 (Dollars per Million Btu) State Primary Energy Electric Power Sector g,h Retail Electricity Total Energy g,i Coal Natural Gas a Petroleum Nuclear Fuel Biomass Total g,h,i Distillate Fuel

  2. Combining local search and backtracking techniques for constraint satisfaction

    SciTech Connect (OSTI)

    Zhang, Jian; Zhang, Hantao

    1996-12-31

    Backtracking techniques are well-known traditional methods for solving many constraint satisfaction problems (CSPs) including the satisfiability (SAT) problem in the propositional logic. In recent years, it has been reported that local search techniques are very effective in solving some large-scale instances of the SAT problem. In this research, we combine the backtracking and local search techniques into a single method for solving SAT and CSPs. When setting a parameter of the method to either of its two extreme values, we obtain the ordinary backtracking procedure or the local search procedure. For some problems, if the parameter takes values in the middle of the two extremes, the new method is much more effective than either backtracking or local search. We tested the method with classical problems like the n-Queens and random SAT instances, as well as some difficult problems from finite mathematics. In particular, using the new method, we solved four open problems in design theory.

  3. Water-quality data for aquifers, streams, and lakes in the vicinity of Keechi, Mount Sylvan, Oakwood, and Palestine salt domes, northeast Texas salt-dome basin

    SciTech Connect (OSTI)

    Carr, J.E.; Halasz, S.J.; Liscum, F.

    1980-11-01

    This report contains water-quality data for aquifers, streams, and lakes in the vicinity of Keechi, Mount Sylvan, Oakwood, and Palestine Salt Domes in the northeast Texas salt-dome basin. Water-quality data were compiled for aquifers in the Wilcox Group, the Carrizo Sand, and the Queen City Sand. The data include analyses for dissolved solids, pH, temperature, hardness, calcium, magnesium, sodium, bicarbonate, chloride, and sulfate. Water-quality and streamflow data were obtained from 63 surface-water sites in the vicinity of the domes. These data include water discharge, specific conductance, pH, water temperature, and dissolved oxygen. Samples were collected at selected sites for analysis of principal and selected minor dissolved constituents.

  4. Elevated tritium levels at the World Trade Center

    SciTech Connect (OSTI)

    Semkow, Thomas M.; Hafner, Ronald S.; Parekh, Pravin P.; Wozniak, Gordon J.; Haines, Douglas K.; Husain, Liaquat; Rabun, Robert L.; Williams, Philip G.

    2002-05-14

    Traces of tritiated water (HTO) were detected at [the]World Trade Center (WTC) ground zero after the 9/11/01 terrorist attack. A method of ultralow-background liquid scintillation counting was used after distilling HTO from the samples. A water sample from the WTC sewer, collected on 9/13/01, contained 0.174 plus or minus 0.074 (2s) nCi/L of HTO. A split water sample, collected on 9/21/01 from the basement of WTC Building 6, contained 3.53 plus or minus 0.17 and 2.83 plus or minus 0.15 nCi/L, respectively. Several water and vegetation samples were analyzed from areas outside the ground zero, located in Manhattan, Brooklyn, Queens, and Kensico Reservoir. No HTO above the background was found in those samples. All these results are well below the levels of concern to human exposure.

  5. Albany Center map_rev36-10

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Bro adw ay Stre et Lib ert y Str ee t Queen Avenue N S1 S2 S3 Locked Gate Locked Gate Locked Gate Locked Gate Locked Gate Locked Gate Walk Gate (locked) Walk Gate (locked) Walk Gate (locked) 0 200 Scale in feet 1 40 33 31 30 29 28 Trailer 2 Trailer 1 27 26 25 23 24 22 20 21 19 18 36 35 37 38 39 34 17 14,15,16 13 7 8 9 10 11 6 12 5 4 2 32 VACANT FEDERAL PROPERTY S4 Gas Sheds Covered Dock Main Gate Security Guard Emergency Gate (Automatic) Locked Gate Locked Gate Walk Gate (locked) Scrap Metal

  6. Apartment Compartmentalization With an Aerosol-Based Sealing Process

    SciTech Connect (OSTI)

    Maxwell, S.; Berger, D.; Harrington, C.

    2015-03-01

    Air sealing of building enclosures is a difficult and time-consuming process. Current methods in new construction require laborers to physically locate small and sometimes large holes in multiple assemblies and then manually seal each of them. The innovation demonstrated under this research study was the automated air sealing and compartmentalization of buildings through the use of an aerosolized sealant, developed by the Western Cooling Efficiency Center at University of California Davis. CARB sought to demonstrate this new technology application in a multifamily building in Queens, NY. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before and after sealing, point-source testing of individual leaks, and pressure measurements in the walls of the target apartment during sealing.

  7. Goos-Hänchen effect and bending of spin wave beams in thin magnetic films

    SciTech Connect (OSTI)

    Gruszecki, P. Krawczyk, M.; Romero-Vivas, J.; Dadoenkova, Yu. S.; Dadoenkova, N. N.; Lyubchanskii, I. L.

    2014-12-15

    For magnon spintronic applications, the detailed knowledge of spin wave (SW) beam dispersion, transmission (reflection) of SWs passing through (reflected from) interfaces, or borders or the scattering of SWs by inhomogeneities is crucial. These wave properties are decisive factors on the usefulness of a particular device. Here, we demonstrate, using micromagnetic simulations supported by an analytical model, that the Goos-Hänchen (GH) shift exists for SW reflecting from thin film edge and that with the effect becomes observable. We show that this effect will exist for a broad range of frequencies in the dipole-exchange range, with the magnetization degree of pinning at the film edge as the crucial parameter, whatever its nature. Moreover, we have also found that the GH effect can be accompanied or even dominating by a bending of the SW beam due to the inhomogeneity of the internal magnetic field. This inhomogeneity, created by demagnetizing field taking place at the film edge, causes gradual change of SWs refractive index. The refraction of the SW beams by the non-uniformity of the magnetic field enables the exploration of graded index magnonics and metamaterial properties for the transmission and processing of information at nanoscale.

  8. Understanding catalysis in a multiphasic two-dimensional transition metal dichalcogenide.

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Chou, Stanley Shihyao; Sai, Na; Lu, Ping; Coker, Eric Nicholas; Liu, Sheng; Artyushkova, Kateryna; Luk, Ting S.; Kaehr, Bryan James; Brinker, C. Jeffrey

    2015-10-07

    Establishing processing–structure–property relationships for monolayer materials is crucial for a range of applications spanning optics, catalysis, electronics and energy. Presently, for molybdenum disulfide, a promising catalyst for artificial photosynthesis, considerable debate surrounds the structure/property relationships of its various allotropes. Here we unambiguously solve the structure of molybdenum disulfide monolayers using high-resolution transmission electron microscopy supported by density functional theory and show lithium intercalation to direct a preferential transformation of the basal plane from 2H (trigonal prismatic) to 1T' (clustered Mo). These changes alter the energetics of molybdenum disulfide interactions with hydrogen (ΔGH), and, with respect to catalysis, the 1T' transformationmore » renders the normally inert basal plane amenable towards hydrogen adsorption and hydrogen evolution. Furthermore, we show basal plane activation of 1T' molybdenum disulfide and a lowering of ΔGH from +1.6 eV for 2H to +0.18 eV for 1T', comparable to 2H molybdenum disulfide edges on Au(111), one of the most active hydrogen evolution catalysts known.« less

  9. Caldicellulosiruptor Core and Pangenomes Reveal Determinants for

    SciTech Connect (OSTI)

    Blumer-Schuette, Sara E.; Giannone, Richard J; Zurawski, Jeffrey V; Ozdemir, Inci; Ma, Qin; Yin, Yanbin; Xu, Ying; Kataeva, Irena; Poole, Farris; Adams, Michael W. W.; Hamilton-Brehm, Scott; Elkins, James G; Larimer, Frank W; Land, Miriam L; Hauser, Loren John; Cottingham, Robert W; Hettich, Robert {Bob} L; Kelly, Robert M

    2012-01-01

    Extremely thermophilic bacteria of the genus Caldicellulosiruptor utilize carbohydrate components of plant cell walls, including cellulose and hemicellulose, facilitated by a diverse set of glycoside hydrolases (GHs). From a biofuel perspective, this capability is crucial for deconstruction of plant biomass into fermentable sugars. While all species from the genus grow on xylan and acidpretreated switchgrass, growth on crystalline cellulose is variable. The basis for this variability was examined using microbiological, genomic, and proteomic analyses of eight globally diverse Caldicellulosiruptor species. The open Caldicellulosiruptor pangenome (4,009 open reading frames [ORFs]) encodes 106 GHs, representing 43 GH families, but only 26 GHs from 17 families are included in the core (noncellulosic) genome (1,543 ORFs). Differentiating the strongly cellulolytic Caldicellulosiruptor species from the others is a specific genomic locus that encodes multidomain cellulases from GH families 9 and 48, which are associated with cellulose-binding modules. This locus also encodes a novel adhesin associated with type IV pili, which was identified in the exoproteome bound to crystalline cellulose. Taking into account the core genomes, pangenomes, and individual genomes, the ancestral Caldicellulosiruptor was likely cellulolytic and evolved, in some cases, into species that lost the ability to degrade crystalline cellulose while maintaining the capacity to hydrolyze amorphous cellulose and hemicellulose.

  10. Fluvial and deltaic facies and environments of the late permian back-reef shelves of the Permian Basin of Texas and New Mexico

    SciTech Connect (OSTI)

    Mazzullo, J. )

    1993-02-01

    The Artesia Group is a sequence of carbonates, evaporites, and clastics that was deposited across the back-reef shelves of the Permian Basin during late Permian time. There has been some controversy over the depositional environments of the clastic members of the Artesia Group and the role of sea level fluctuations in their accumulation. However, the results of a regional core study of the Queen Formation of the Artesia Group indicate that they were largely deposited in desert fluvial and deltaic environments during low-stands of sea level. Three fluvial-deltaic facies are recognized within the clastic members of the Queen. The first consists of medium to very find sandstones and silty sandstones with cross-beds, ripple cross-laminae, and planar and wavy laminae. This facies forms wavy sheets that thicken and thin along linear trends, and was deposited in sandy braided streams. The second facies consists of very find to fine sandstones, silty sandstones, and siltstones with ripple cross-laminae, planar and wavy laminae, cross-beds, clay drapes and pedogenetic cutans, as well as siltstones and silty mudstones with haloturbation structures and evaporite nodules. This facies forms thick planar sheets, and was deposited in fluvial sandflats and adjacent fluvial-dominated continental sabkhas. The third facies consists of cyclic deposits of haloturbated silty mudstones that grade into siltstones and very fine sandstones with crossbeds, planar and wavy laminae, haloturbation structures and evaporite nodules. Each cycle forms a lobate body that is bounded by carbonates or evaporites and which was deposited in sheet deltas that formed along the landward margins of a back-reef lagoon.

  11. Distributed Sensor Coordination for Advanced Energy Systems

    SciTech Connect (OSTI)

    Tumer, Kagan

    2013-07-31

    The ability to collect key system level information is critical to the safe, efficient and reli- able operation of advanced energy systems. With recent advances in sensor development, it is now possible to push some level of decision making directly to computationally sophisticated sensors, rather than wait for data to arrive to a massive centralized location before a decision is made. This type of approach relies on networked sensors (called agents from here on) to actively collect and process data, and provide key control deci- sions to significantly improve both the quality/relevance of the collected data and the as- sociating decision making. The technological bottlenecks for such sensor networks stem from a lack of mathematics and algorithms to manage the systems, rather than difficulties associated with building and deploying them. Indeed, traditional sensor coordination strategies do not provide adequate solutions for this problem. Passive data collection methods (e.g., large sensor webs) can scale to large systems, but are generally not suited to highly dynamic environments, such as ad- vanced energy systems, where crucial decisions may need to be reached quickly and lo- cally. Approaches based on local decisions on the other hand cannot guarantee that each agent performing its task (maximize an agent objective) will lead to good network wide solution (maximize a network objective) without invoking cumbersome coordination rou- tines. There is currently a lack of algorithms that will enable self-organization and blend the efficiency of local decision making with the system level guarantees of global decision making, particularly when the systems operate in dynamic and stochastic environments. In this work we addressed this critical gap and provided a comprehensive solution to the problem of sensor coordination to ensure the safe, reliable, and robust operation of advanced energy systems. The differentiating aspect of the proposed work is in shift- ing the

  12. FY 2008 Annual Performance Report

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Hyd rog en Tan k Res earc h, LLN L PH EN IX Ex pe rim en t, BN L Fu el Ce ll Re sea rch , AN L Ca rb on Se qu es tra tio n Re se ar ch , PN NL Hi gh Ex pl os iv es Ap pl ic at io ns Fa ci lit y, LL NL Com put er Sim ulat ion The ater , LAN L Al ga e Re se ar ch , NR EL Ad va nc ed Bio fue ls Re se arc h, LB NL T ra in in g Nuc lear Mat eria ls Sto rag e, SRS C o a l G a s if ic a ti o n R e s e a rc h , P N N L Cli ma te Mo de lin g, OR NL AnnuAl PerformAnce rePort fY 2008 Table of Contents

  13. Low rate entrainment feeder for fine solids

    SciTech Connect (OSTI)

    Scott, D.S.; Piskorz, J.

    1982-08-01

    A mechanically stirred entrainment-type feeder for fine solids has been developed which will give rates constant to +/- 5% for 1 h or more. The feeder was constructed in connection with a mini-fluidized bed flash pyrolysis project for both biomass and Canadian coals. It has been used to feed coal, sawdust and ground bark in sizes below 600 micro m at rates as low as 6 g/h. Gas to solids weight ratios obtained were from about 3:1 to 1:3. The effect on feed rates of most of the operating and geometric parameters was investigated at low feed rates. A mechanism for control of the feed rate was tested and found to be satisfactory.

  14. A=6Be (1974AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    4AJ01) (See Energy Level Diagrams for 6Be) GENERAL: See also (1966LA04) and Table 6.7 [Table of Energy Levels] (in PDF or PS). Model calculations: (1966BA26, 1968BA35, 1968FA1B, 1968VA1H, 1969TH1C, 1970LA1D). Other topics: (1965GO1D, 1966GO1B, 1970FO1B, 1972AN05, 1972CA37, 1972GH1A, 1972JA14, 1973WE18). 1. (a) 3He(3He, γ)6Be Qm = 11.488 Eb = 11.488 (b) 3He(3He, p)5Li Qm = 10.89 (c) 3He(3He, 2p)4He Qm = 12.8601 (d) 3He(3He, 3p)3H Qm = -6.9546 (e) 3He(3He, 3He)3He (f) 3He(3He, d)4Li Qm = -8.4 The

  15. A=6He (1979AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    9AJ01) (See Energy Level Diagrams for 6He) GENERAL: See also (1974AJ01) and Table 6.1 [Table of Energy Levels] (in PDF or PS) here. Model calculations: (1974GH01, 1974IR04, 1975FI1C, 1975FI1D, 1975VE01, 1976CE03, 1976IR1B). Astrophysical questions: (1976VI1A). Electromagnetic interactions: (1975VE01). Special reactions: (1974BO08, 1975FE1A, 1975ZE01, 1976BO08, 1976VA29, 1977FE1B, 1977YA1A). Muon and neutrino capture and reactions: (1973MU1B, 1974CA04, 1975DO1F, 1976WA02, 1977PR1B, 1978DE15,

  16. A=6Li (1979AJ01)

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    79AJ01) (See Energy Level Diagrams for 6Li) GENERAL: See also (1974AJ01) and Table 6.2 [Table of Energy Levels] (in PDF or PS) here. Shell model: (1974KA11, 1975DI04, 1975GO1B, 1975VE01, 1976CE03, 1976GH1A). Collective, rotational and deformed models: (1974BO25). Cluster and α-particle models: (1972KR1A, 1973DO09, 1973LI23, 1974BA30, 1974GR24, 1974JA1K, 1974KA11, 1974NO03, 1974PA1B, 1974SH08, 1974WO1B, 1975BL1C, 1975GO08, 1975GR26, 1975HA48, 1975KR1A, 1975LE1A, 1975LI1C, 1975MI09, 1975NO03,

  17. Dynamics of compressional Mach cones in a strongly coupled complex plasma

    SciTech Connect (OSTI)

    Bandyopadhyay, P., E-mail: pintu@ipr.res.in; Dey, R.; Sen, Abhijit [Institute for Plasma Research, Bhat, Gandhinagar-382428 (India); Kadyan, Sangeeta [Department of Physics, Maharshi Dayanand University, Rohtak 124001 (India)

    2014-10-15

    Using a Generalised-Hydrodynamic (GH) fluid model, we study the influence of strong coupling induced modification of the fluid compressibility on the dynamics of compressional Mach cones in a dusty plasma medium. A significant structural change of lateral wakes for a given Mach number and Epstein drag force is found in the strongly coupled regime. With the increase of fluid compressibility, the peak amplitude of the normalised perturbed dust density first increases and then decreases monotonically after reaching its maximum value. It is also noticed that the opening angle of the cone structure decreases with the increase of the compressibility of the medium and the arm of the Mach cone breaks up into small structures in the velocity vector profile when the coupling between the dust particles increases.

  18. S U M M A R I E S U.S. Energy Information Administration | State Energy Data 2014: Prices and Expenditures

    Gasoline and Diesel Fuel Update (EIA)

    Prices and Expenditures 10 Table E8. Primary Energy, Electricity, and Total Energy Expenditure Estimates, 2014 (Million Dollars) State Primary Energy Electric Power Sector g,h Retail Electricity Total Energy g,i Coal Natural Gas a Petroleum Nuclear Fuel Biomass Total g,h,i Distillate Fuel Oil Jet Fuel b LPG c Motor Gasoline d Residual Fuel Oil Other e Total Wood and Waste f Alabama 1,677.3 3,521.2 3,718.3 283.1 226.6 8,039.9 97.2 713.3 13,078.4 344.2 498.0 19,119.2 -3,336.0 8,363.3 24,146.5

  19. S U M M A R I E S U.S. Energy Information Administration | State Energy Data 2014: Prices and Expenditures

    Gasoline and Diesel Fuel Update (EIA)

    3 Table E1. Primary Energy, Electricity, and Total Energy Price Estimates, 2014 (Dollars per Million Btu) State Primary Energy Electric Power Sector g,h Retail Electricity Total Energy g,i Coal Natural Gas a Petroleum Nuclear Fuel Biomass Total g,h,i Distillate Fuel Oil Jet Fuel b LPG c Motor Gasoline d Residual Fuel Oil Other e Total Wood and Waste f Alabama 2.91 5.81 25.88 19.92 26.79 25.88 12.59 28.08 25.64 0.80 3.43 8.43 2.60 27.20 18.64 Alaska 4.87 6.99 28.53 20.97 27.65 33.67 18.87 19.35

  20. Use of external metabolizing systems when testing for endocrine disruption in the T-screen assay

    SciTech Connect (OSTI)

    Taxvig, Camilla Olesen, Pelle Thonning; Nellemann, Christine

    2011-02-01

    Although, it is well-established that information on the metabolism of a substance is important in the evaluation of its toxic potential, there is limited experience with incorporating metabolic aspects into in vitro tests for endocrine disrupters. The aim of the current study was a) to study different in vitro systems for biotransformation of ten known endocrine disrupting chemicals (EDs): five azole fungicides, three parabens and 2 phthalates, b) to determine possible changes in the ability of the EDs to bind and activate the thyroid receptor (TR) in the in vitro T-screen assay after biotransformation and c) to investigate the endogenous metabolic capacity of the GH3 cells, the cell line used in the T-screen assay, which is a proliferation assay used for the in vitro detection of agonistic and antagonistic properties of compounds at the level of the TR. The two in vitro metabolizing systems tested the human liver S9 mix and the PCB-induced rat microsomes gave an almost complete metabolic transformation of the tested parabens and phthalates. No marked difference the effects in the T-screen assay was observed between the parent compounds and the effects of the tested metabolic extracts. The GH3 cells themselves significantly metabolized the two tested phthalates dimethyl phthalate (DMP) and diethyl phthalate (DEP). Overall the results and qualitative data from the current study show that an in vitro metabolizing system using liver S9 or microsomes could be a convenient method for the incorporation of metabolic and toxicokinetic aspects into in vitro testing for endocrine disrupting effects.

  1. Comparative Analysis of Carbon Plasma in Arc and RF Reactors

    SciTech Connect (OSTI)

    Todorovic-Markovic, B.; Markovic, Z.; Mohai, I.; Szepvolgyi, J.

    2004-12-01

    Results on studies of molecular spectra emitted in the initial stages of fullerene formation during the processing of graphite powder in induction RF reactor and evaporation of graphite electrodes in arc reactor are presented in this paper. It was found that C2 radicals were dominant molecular species in both plasmas. C2 radicals have an important role in the process of fullerene synthesis. The rotational-vibrational temperatures of C2 and CN species were calculated by fitting the experimental spectra to the simulated ones. The results of optical emission study of C2 radicals generated in carbon arc plasma have shown that rotational temperature of C2 species depends on carbon concentration and current intensity significantly. The optical emission study of induction RF plasma and SEM analysis of graphite powder before and after plasma treatment have shown that evaporation of the processed graphite powder depends on feed rate and composition of gas phase significantly. Based on the obtained results, it was concluded that in the plasma region CN radicals could be formed by the reaction of C2 species with atomic nitrogen at smaller loads. At larger feed rate of graphite powder, CN species were produced by surface reaction of the hot carbon particles with nitrogen atoms. The presence of nitrogen in induction RF plasma reduces the fullerene yield significantly. The fullerene yield obtained in two different reactors was: 13% in arc reactor and 4.1% in induction RF reactor. However, the fullerene production rate was higher in induction RF reactor-6.4 g/h versus 1.7 g/h in arc reactor.

  2. Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Walker, Johnnie A.; Takasuka, Taichi E.; Deng, Kai; Bianchetti, Christopher M.; Udell, Hannah S.; Prom, Ben M.; Kim, Hyunkee; Adams, Paul D.; Northen, Trent R.; Fox, Brian G.

    2015-12-21

    Carbohydrate binding modules (CBMs) bind polysaccharides and help target glycoside hydrolases catalytic domains to their appropriate carbohydrate substrates. To better understand how CBMs can improve cellulolytic enzyme reactivity, representatives from each of the 18 families of CBM found in Ruminoclostridium thermocellum were fused to the multifunctional GH5 catalytic domain of CelE (Cthe_0797, CelEcc), which can hydrolyze numerous types of polysaccharides including cellulose, mannan, and xylan. Since CelE is a cellulosomal enzyme, none of these fusions to a CBM previously existed. CelEcc_CBM fusions were assayed for their ability to hydrolyze cellulose, lichenan, xylan, and mannan. Several CelEcc_CBM fusions showed enhanced hydrolyticmore » activity with different substrates relative to the fusion to CBM3a from the cellulosome scaffoldin, which has high affinity for binding to crystalline cellulose. Additional binding studies and quantitative catalysis studies using nanostructure-initiator mass spectrometry (NIMS) were carried out with the CBM3a, CBM6, CBM30, and CBM44 fusion enzymes. In general, and consistent with observations of others, enhanced enzyme reactivity was correlated with moderate binding affinity of the CBM. Numerical analysis of reaction time courses showed that CelEcc_CBM44, a combination of a multifunctional enzyme domain with a CBM having broad binding specificity, gave the fastest rates for hydrolysis of both the hexose and pentose fractions of ionic-liquid pretreated switchgrass. In conclusion, we have shown that fusions of different CBMs to a single multifunctional GH5 catalytic domain can increase its rate of reaction with different pure polysaccharides and with pretreated biomass. This fusion approach, incorporating domains with broad specificity for binding and catalysis, provides a new avenue to improve reactivity of simple combinations of enzymes within the complexity of plant biomass.« less

  3. Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules

    SciTech Connect (OSTI)

    Walker, Johnnie A.; Takasuka, Taichi E.; Deng, Kai; Bianchetti, Christopher M.; Udell, Hannah S.; Prom, Ben M.; Kim, Hyunkee; Adams, Paul D.; Northen, Trent R.; Fox, Brian G.

    2015-12-21

    Carbohydrate binding modules (CBMs) bind polysaccharides and help target glycoside hydrolases catalytic domains to their appropriate carbohydrate substrates. To better understand how CBMs can improve cellulolytic enzyme reactivity, representatives from each of the 18 families of CBM found in Ruminoclostridium thermocellum were fused to the multifunctional GH5 catalytic domain of CelE (Cthe_0797, CelEcc), which can hydrolyze numerous types of polysaccharides including cellulose, mannan, and xylan. Since CelE is a cellulosomal enzyme, none of these fusions to a CBM previously existed. CelEcc_CBM fusions were assayed for their ability to hydrolyze cellulose, lichenan, xylan, and mannan. Several CelEcc_CBM fusions showed enhanced hydrolytic activity with different substrates relative to the fusion to CBM3a from the cellulosome scaffoldin, which has high affinity for binding to crystalline cellulose. Additional binding studies and quantitative catalysis studies using nanostructure-initiator mass spectrometry (NIMS) were carried out with the CBM3a, CBM6, CBM30, and CBM44 fusion enzymes. In general, and consistent with observations of others, enhanced enzyme reactivity was correlated with moderate binding affinity of the CBM. Numerical analysis of reaction time courses showed that CelEcc_CBM44, a combination of a multifunctional enzyme domain with a CBM having broad binding specificity, gave the fastest rates for hydrolysis of both the hexose and pentose fractions of ionic-liquid pretreated switchgrass. In conclusion, we have shown that fusions of different CBMs to a single multifunctional GH5 catalytic domain can increase its rate of reaction with different pure polysaccharides and with pretreated biomass. This fusion approach, incorporating domains with broad specificity for binding and catalysis, provides a new avenue to improve reactivity of simple combinations of enzymes within the complexity of plant biomass.

  4. Flexible Ultra Moisture Barrier Film for Thin-Film Photovoltaic Applications

    SciTech Connect (OSTI)

    David M. Dean

    2012-10-30

    Flexible Thin-film photovoltaic (TFPV) is a low cost alternative to incumbent c-Si PV products as it requires less volume of costly semiconductor materials and it can potentially reduce installation cost. Among the TFPV options, copper indium gallium diselenide (CIGS) has the highest efficiency and is believed to be one of the most attractive candidates to achieve PV cost reduction. However, CIGS cells are very moisture sensitive and require module water vapor transmission rate (WVTR) of less than 1x10-4 gram of water per square meter per day (g-H2O/m2/day). Successful development and commercialization of flexible transparent ultra moisture barrier film is the key to enable flexible CIGS TFPV products, and thus enable ultimate PV cost reduction. At DuPont, we have demonstrated at lab scale that we can successfully make polymer-based flexible transparent ultra moisture barrier film by depositing alumina on polymer films using atomic layer deposition (ALD) technology. The layer by layer ALD approach results in uniform and amorphous structure which effectively reduces pinhole density of the inorganic coating on the polymer, and thus allow the fabrication of flexible barrier film with WVTR of 10-5 g-H2O/m2/day. Currently ALD is a time-consuming process suitable only for high-value, relatively small substrates. To successfully commercialize the ALD-on-plastic technology for the PV industry, there is the need to scale up this technology and improve throughput. The goal of this contract work was to build a prototype demonstrating that the ALD technology could be scaled-up for commercial use. Unfortunately, the prototype failed to produce an ultra-barrier film by the close of the project.

  5. Extracting maximum petrophysical and geological information from a limited reservoir database

    SciTech Connect (OSTI)

    Ali, M.; Chawathe, A.; Ouenes, A.

    1997-08-01

    The characterization of old fields lacking sufficient core and log data is a challenging task. This paper describes a methodology that uses new and conventional tools to build a reliable reservoir model for the Sulimar Queen field. At the fine scale, permeability measured on a fine grid with a minipermeameter was used in conjunction with the petrographic data collected on multiple thin sections. The use of regression analysis and a newly developed fuzzy logic algorithm led to the identification of key petrographic elements which control permeability. At the log scale, old gamma ray logs were first rescaled/calibrated throughout the entire field for consistency and reliability using only four modem logs. Using data from one cored well and the rescaled gamma ray logs, correlations between core porosity, permeability, total water content and gamma ray were developed to complete the small scale characterization. At the reservoir scale, outcrop data and the rescaled gamma logs were used to define the reservoir structure over an area of ten square miles where only 36 wells were available. Given the structure, the rescaled gamma ray logs were used to build the reservoir volume by identifying the flow units and their continuity. Finally, history-matching results constrained to the primary production were used to estimate the dynamic reservoir properties such as relative permeabilities to complete the characterization. The obtained reservoir model was tested by forecasting the waterflood performance and which was in good agreement with the actual performance.

  6. Geology of north-central Delaware basin, Eddy and Lea Counties, New Mexico: its hydrocarbon potential, focusing on 12 townships centered on WIPP site

    SciTech Connect (OSTI)

    Cheeseman, R.J.

    1986-03-01

    The Waste Isolation Pilot Plant (WIPP) site is located within the Carlsbad potash mining area, southeastern New Mexico, about 20 mi east of Carlsbad. Structurally, the WIPP site is located in the north-central part of the Delaware basin, which yields hydrocarbon production from the following: the Ordovician Ellenburger; the Pennsylvanian Morrow (gas), Atoka (oil and gas), and Strawn (reef oil) intervals; the Wolfcamp (gas) and Bone Spring (oil) formations of lowermost Permian; the Permian Yates (800-3500 ft deep), Queen, and Seven Rivers Formations; and the Delaware Mountain Group (4700-5200 ft deep). Structure contour maps demonstrate favorable Bone Spring conditions north of the WIPP site and the centrally located Delaware targets, as well as important Morrow development in the southern part. Five prospects are explored, and two are especially promising. Five anticlinal trends in this 12-township area bear field names as a result of production: Big Eddy, South Salt Lake, Cabin Lake, Los Medanos, and Sand Dunes. The Department of Energy's WIPP project is a planned repository for nuclear waste; despite centering on a deep dry hole, it occurs just northeast of productive Morrow formation. Whereas the successful tests seem concentrated on the structural highs, significant wells produce offtrend; the WIPP site lies in a syncline.

  7. Lower Pennsylvanian reservoirs of Parkway-Empire south field area, Eddy County, New Mexico

    SciTech Connect (OSTI)

    James, A.D.

    1984-01-01

    The Parkway-Empire area is located on the Northwest shelf in central Eddy County, approximately 15 mi (24 km) northeast of Carlsbad, New Mexico. This area produces oil and gas from the lower and middle Morrow sandstones, Atoka sandstones, and Strawn limestones. Oil is also produced from the Queen and Seven Rivers sandstones, and the Grayburg, San Andres, and Wolfcamp dolomites. All of the these zones are productive from stratigraphic traps. The lower Morrow sandstones occur at a depth of about 11,400 ft (3,474 m). In the Parkway area, the middle Morrow sandstones are thought to be a transgressive series of marine beaches and submarine bars which trend toward the northeast, parallel to depositional strike. Stratigraphic traps are created in the lower and middle Morrow sandstones by variations in cementation and depositional patterns. Productive Atoka sandstones occur at a depth of approximately 10,700 ft (3,261 m). Strawn limestones produce from a series of small, low-relief algal banks developed along depositional strike to the northeast. The Strawn limestone is about 300 ft (91 m) thick and occurs between 10,250 and 10,500 ft (3,124 and 3,200 m). Wells in this area have typical ultimate recoveries of between 1.0 and 3.0 bcf of gas. The combination of stacked reservoirs and good production makes this area of the Morrow trend especially attractive.

  8. Oil and gas developments in South America, Central America, Caribbean area, and Mexico in 1987

    SciTech Connect (OSTI)

    Wiman, W.D.

    1988-10-01

    Exploration activity in South America, Central America, the Caribbean area, and Mexico in 1987 showed significant increases in seismic acquisition in Belize, Bolivia, Brazil, Costa Rica, Guatemala, Mexico, Paraguay, and Peru, and a decrease in Chile and Venezuela. Exploratory drilling increased in most major producing countries but was accompanied by a decline in development drilling. Most of the increase could be attributed to private companies fulfilling obligations under risk contracts; however, state oil companies in Bolivia, Chile, and Colombia showed significant increased activity, with only Mexico showing a decrease. Colombia again had a dramatic increase in production (29% from 1986). Noteworthy discoveries were made in Bolivia (Villamontes-1); Brazil, in the Solimoes basin (1-RUC-1-AM); Chile (Rio Honda-1); Colombia, in the Llanos basin (Austral-1, La Reforma-1, Libertad Norte-1, Cravo Este-1, and Cano Yarumal-1), in the Upper Magdalena basin (Toldado-1 and Los Mangos-1); Ecuador (Frontera-1, a joint-exploration venture with Colombia); Mexico, in the Chiapas-Tabasco region (Guacho-1 and Iridi-1), in the Frontera Norte area (Huatempo-1); Peru, in the Madre de Dios basin (Armihuari-4X); Trinidad (West East Queen's Beach-1); and Venezuela (Musipan-1X). Brazil's upper Amazon (Solimoes basin) discovery, Colombia's Upper Magdalena basin discoveries Toldado-1 and Los Mangos-1, Mexico's Chiapas-Tabasco discoveries, Peru's confirmation of the giant Cashiriari discovery of 1986, and Venezuela's success in Monagas state were the highlights of 1987. 5 figs., 8 tabs.

  9. Fracture identification and evaluation using borehole imaging and full wave form logs in the Permian basin

    SciTech Connect (OSTI)

    Sanders, L. )

    1994-03-01

    The borehole imaging and acoustic full wave form logs provide an excellent means for identifying and evaluating naturally occurring fractures. The natural fractures can provide the porosity and permeability essential for a productive reservoir. The detection of these fractures may be accomplished by tow types of wireline logging tools: borehole imaging devices and acoustic full wave form tools. The borehole imaging tools produce images based upon the electromagnetic or the acoustic properties of the borehole wall. Fractures will appear as darker images that are distinct from the nonfracture formation. These images are coupled with a reference azimuth that allows for the determination of the orientation of the fracture image. The acoustic full wave form logs are used to detect fractures by analyzing various acoustic properties of the formation. The travel time, amplitude, and frequency responses of fractured formations differ remarkably from the responses of nonfractured formations because of the reduction of the acoustic energy in the fractures. The various field examples from the Queen sandstone to the Ellenburger formation demonstrate the advantages and disadvantages unique to the borehole imaging and the acoustic full wave form devices. Within this geologic framework, comparisons are made among the data extracted from whole cores, borehole imaging devices, and the acoustic full wave form tools in establishing a systematic approach for the identification and evaluation of fractures.

  10. Maintaining urban gas systems demands special technologies

    SciTech Connect (OSTI)

    Anglero, T.F. )

    1994-04-01

    Brooklyn Union Gas Co. has been providing gas to 50% of the population of New York City for the last 100 years. The company has constructed an elaborate gas distribution network that includes a gas main under nearly every city street in a service territory that includes Brooklyn, Staten Island and parts of Queens. Conventional ways of pipeline construction and maintenance are inadequate in today's environment of heightened competition, increased regulations and, most importantly, demanding customer expectations of quality service. As a result, Brooklyn Union Gas must use special construction and maintenance methods in its operations, and in particular trenchless technologies. Over the past 10 years the company has paid close attention to developing a variety of trenchless techniques. Like many gas distribution companies providing service in densely populated urban areas, Brooklyn Union must operate and maintain its gas distribution network in a challenging environment of increasing governmental regulation and escalating field construction costs. Technological innovation is not a luxury, but instead a necessity to achieve corporate growth, regulatory compliance and greater customer satisfaction. Trenchless technologies offset rising pipe installation costs and provide benefits both to the customer and the company. Of special value to Brooklyn Union is the development of systems that renovate old metal pipes by lining. Such techniques are described.

  11. Automated software system reduces leak investigation time

    SciTech Connect (OSTI)

    Not Available

    1994-12-01

    With more than a million customers in the New York City boroughs of Brooklyn, Queens, and Staten Island, Brooklyn Union Gas Co. is one of the largest natural gas distributors in the US. In September 1993, the company began working with MapFrame Corp., Dallas, to develop a new program to automate their underground leak investigation process. Automating this process would greatly reduce time in finding a leaking main and result in improved customer relations and productivity gains. Keys to the success of this program were in using pen computers, wireless communications, and a jointly-developed software. MapFrame and Brooklyn Union Gas agreed that the application should allow onsite users to: display a model of the affected area using map data to show streets, services, buildings, manholes, street lights, and other landmarks; record bar hole readings, manipulate data, and use diagnostic tools to analyze gas concentrations; update leak records (per legal requirements); and integrate Map Data. The paper reviews this program.

  12. Observations of shock-induced reaction in liquid bromoform up to 11 GPA

    SciTech Connect (OSTI)

    Sheffield, S.A.; Gustavsen, R.L.; Alcon, R.R.

    1995-09-01

    Shock measurements on bromoform (CHBr{sub 3}) over the past 33 years at Los Alamos have led to speculation that this material undergoes a shock-induced reaction. Ramsay observed that it became opaque after a 1 to 2 {micro}s induction time when shocked to pressures above 6 GPa. McQueen and Isaak observed that it is a strong light emitter above 25 GPa. Hugoniot data start to deviate from the anticipated liquid Hugoniot at pressures above 10 GPa. The authors have used electromagnetic particle velocity gauging to measure wave profiles in shocked liquid bromoform. At pressures below 9 GPa, there is no mechanical evidence of reaction. At a pressure slightly above 10 GPa, the observed wave profiles are similar to those observed in initiating liquid explosives such as nitromethane. Their characteristics are completely different from the two-wave structures observed in shocked liquids where the products are more dense than the reactants. As with explosives, a reaction producing products which are less dense than the reactants is indicated. BKW calculations also indicate that a detonation type reaction may be possible.

  13. Technology Solutions Case Study: Apartment Compartmentalization with an Aerosol-Based Sealing Process

    SciTech Connect (OSTI)

    2015-07-01

    Air sealing of building enclosures is a difficult and time-consuming process. Current methods in new construction require laborers to physically locate small and sometimes large holes in multiple assemblies and then manually seal each of them. This research study by Building America team Consortium for Advanced Residential Buildings demonstrated the automated air sealing and compartmentalization of buildings through the use of an aerosolized sealant developed by the Western Cooling Efficiency Center at University of California Davis. CARB demonstrated this new technology application in a multifamily building in Queens, NY. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before and after sealing, point-source testing of individual leaks, and pressure measurements in the walls of the target apartment during sealing. Aerosolized sealing was successful by several measures in this study. Many individual leaks that are labor-intensive to address separately were well sealed by the aerosol particles. In addition, many diffuse leaks that are difficult to identify and treat were also sealed. The aerosol-based sealing process resulted in an average reduction of 71% in air leakage across three apartments and an average apartment airtightness of 0.08 CFM50/SF of enclosure area.

  14. An Easy Tool to Predict Survival in Patients Receiving Radiation Therapy for Painful Bone Metastases

    SciTech Connect (OSTI)

    Westhoff, Paulien G.; Graeff, Alexander de; Monninkhof, Evelyn M.; Bollen, Laurens; Dijkstra, Sander P.; Steen-Banasik, Elzbieta M. van der; Vulpen, Marco van; Leer, Jan Willem H.; Marijnen, Corrie A.; Linden, Yvette M. van der

    2014-11-15

    Purpose: Patients with bone metastases have a widely varying survival. A reliable estimation of survival is needed for appropriate treatment strategies. Our goal was to assess the value of simple prognostic factors, namely, patient and tumor characteristics, Karnofsky performance status (KPS), and patient-reported scores of pain and quality of life, to predict survival in patients with painful bone metastases. Methods and Materials: In the Dutch Bone Metastasis Study, 1157 patients were treated with radiation therapy for painful bone metastases. At randomization, physicians determined the KPS; patients rated general health on a visual analogue scale (VAS-gh), valuation of life on a verbal rating scale (VRS-vl) and pain intensity. To assess the predictive value of the variables, we used multivariate Cox proportional hazard analyses and C-statistics for discriminative value. Of the final model, calibration was assessed. External validation was performed on a dataset of 934 patients who were treated with radiation therapy for vertebral metastases. Results: Patients had mainly breast (39%), prostate (23%), or lung cancer (25%). After a maximum of 142 weeks' follow-up, 74% of patients had died. The best predictive model included sex, primary tumor, visceral metastases, KPS, VAS-gh, and VRS-vl (C-statistic = 0.72, 95% CI = 0.70-0.74). A reduced model, with only KPS and primary tumor, showed comparable discriminative capacity (C-statistic = 0.71, 95% CI = 0.69-0.72). External validation showed a C-statistic of 0.72 (95% CI = 0.70-0.73). Calibration of the derivation and the validation dataset showed underestimation of survival. Conclusion: In predicting survival in patients with painful bone metastases, KPS combined with primary tumor was comparable to a more complex model. Considering the amount of variables in complex models and the additional burden on patients, the simple model is preferred for daily use. In addition, a risk table for survival is provided.

  15. The Structural Basis of Substrate Recognition in an exo-b-d-glucosaminidase Involved in Chitosan Hydrolysis

    SciTech Connect (OSTI)

    Van Bueren, A.; Ghinet, M; Gregg, K; Fleury, A; Brzezinski, R; Boraston, A

    2009-01-01

    Family 2 of the glycoside hydrolase classification is one of the largest families. Structurally characterized members of this family include enzymes with ?-galactosidase activity (Escherichia coli LacZ), ?-glucuronidase activity (Homo sapiens GusB), and ?-mannosidase activity (Bacteroides thetaiotaomicron BtMan2A). Here, we describe the structure of a family 2 glycoside hydrolase, CsxA, from Amycolatopsis orientalis that has exo-?-d-glucosaminidase (exo-chitosanase) activity. Analysis of a product complex (1.85 A resolution) reveals a unique negatively charged pocket that specifically accommodates the nitrogen of nonreducing end glucosamine residues, allowing this enzyme to discriminate between glucose and glucosamine. This also provides structural evidence for the role of E541 as the catalytic nucleophile and D469 as the catalytic acid/base. The structures of an E541A mutant in complex with a natural ?-1,4-d-glucosamine tetrasaccharide substrate and both E541A and D469A mutants in complex with a pNP-?-d-glucosaminide synthetic substrate provide insight into interactions in the + 1 subsite of this enzyme. Overall, a comparison with the active sites of other GH2 enzymes highlights the unique architecture of the CsxA active site, which imparts specificity for its cationic substrate.

  16. The Structural Basis of Substrate Recognition in an exo-beta-d-Glucosaminidase Involved in Chitosan Hydrolysis

    SciTech Connect (OSTI)

    Lammerts van Bueren, A.; Ghinet, M; Gregg, K; Fleury, A; Brzezinski, R; Boraston, A

    2009-01-01

    Family 2 of the glycoside hydrolase classification is one of the largest families. Structurally characterized members of this family include enzymes with beta-galactosidase activity (Escherichia coli LacZ), beta-glucuronidase activity (Homo sapiens GusB), and beta-mannosidase activity (Bacteroides thetaiotaomicron BtMan2A). Here, we describe the structure of a family 2 glycoside hydrolase, CsxA, from Amycolatopsis orientalis that has exo-beta-D-glucosaminidase (exo-chitosanase) activity. Analysis of a product complex (1.85 A resolution) reveals a unique negatively charged pocket that specifically accommodates the nitrogen of nonreducing end glucosamine residues, allowing this enzyme to discriminate between glucose and glucosamine. This also provides structural evidence for the role of E541 as the catalytic nucleophile and D469 as the catalytic acid/base. The structures of an E541A mutant in complex with a natural beta-1,4-D-glucosamine tetrasaccharide substrate and both E541A and D469A mutants in complex with a pNP-beta-D-glucosaminide synthetic substrate provide insight into interactions in the +1 subsite of this enzyme. Overall, a comparison with the active sites of other GH2 enzymes highlights the unique architecture of the CsxA active site, which imparts specificity for its cationic substrate.

  17. Hydrogen Production in Radioactive Solutions in the Defense Waste Processing Facility

    SciTech Connect (OSTI)

    CRAWFORD, CHARLES L.

    2004-05-26

    In the radioactive slurries and solutions to be processed in the Defense Waste Processing Facility (DWPF), hydrogen will be produced continuously by radiolysis. This production results from alpha, beta, and gamma rays from decay of radionuclides in the slurries and solutions interacting with the water. More than 1000 research reports have published data concerning this radiolytic production. The results of these studies have been reviewed in a comprehensive monograph. Information about radiolytic hydrogen production from the different process tanks is necessary to determine air purge rates necessary to prevent flammable mixtures from accumulating in the vapor spaces above these tanks. Radiolytic hydrogen production rates are usually presented in terms of G values or molecules of hydrogen produced per 100ev of radioactive decay energy absorbed by the slurry or solution. With the G value for hydrogen production, G(H2), for a particular slurry and the concentrations of radioactive species in that slurry, the rate of H2 production for that slurry can be calculated. An earlier investigation estimated that the maximum rate that hydrogen could be produced from the sludge slurry stream to the DWPF is with a G value of 0.45 molecules per 100ev of radioactive decay energy sorbed by the slurry.

  18. Optic-microwave mixing velocimeter for superhigh velocity measurement

    SciTech Connect (OSTI)

    Weng Jidong; Wang Xiang; Tao Tianjiong; Liu Cangli; Tan Hua

    2011-12-15

    The phenomenon that a light beam reflected off a moving object experiences a Doppler shift in its frequency underlies practical interferometric techniques for remote velocity measurements, such as velocity interferometer system for any reflector (VISAR), displacement interferometer system for any reflector (DISAR), and photonic Doppler velocimetry (PDV). While VISAR velocimeters are often bewildered by the fringe loss upon high-acceleration dynamic process diagnosis, the optic-fiber velocimeters such as DISAR and PDV, on the other hand, are puzzled by high velocity measurement over 10 km/s, due to the demand for the high bandwidth digitizer. Here, we describe a new optic-microwave mixing velocimeter (OMV) for super-high velocity measurements. By using currently available commercial microwave products, we have constructed a simple, compact, and reliable OMV device, and have successfully obtained, with a digitizer of bandwidth 6 GH only, the precise velocity history of an aluminum flyer plate being accelerated up to 11.2 km/s in a three stage gas-gun experiment.

  19. Prevalence of persistent cough and phlegm in young adults in relation to long-term ambient sulfur oxide exposure

    SciTech Connect (OSTI)

    Chapman, R.S.; Calafiore, D.C.; Hasselblad, V.

    1985-01-01

    In early 1976, a survey of persistent co gh and plegma (PCP) prevalence was conducted in 5623 young adults in four Utah communities. Over the previous five years, community specific mean sulfur dioxide levels had been 11, 18, 36, and 115 ug/mT. Corresponding mean suspended sulfate levels had been 5, 7, 8, and 14 g/mT No intercommunity exposure gradient of total suspended particulates or suspended nitrates was observed. In mothers, PCP prevalence among non-smokers was 4.2% in the high-exposure community and about 2.0% in all other communities. In smoking mothers, PCP prevalence was 21.8% in the high-exposure community and about 15.0% elsewhere. In fathers, PCP prevalence among non-smokers was about 8.0% in the high-exposure community and averaged about 3.0% elsewhere. In smoking fathers, PCP prevalence was less strongly associated with sulfur oxide exposure. PCP prevalence rates estimated in a categorical logistic regression model were qualitatively consistent with the prevalences presented above.

  20. Intercalation of organic molecules in 2D copper (II) nitroprusside: Intermolecular interactions and magnetic properties

    SciTech Connect (OSTI)

    Osiry, H.; Cano, A.; Lemus-Santana, A.A.; Rodríguez, A.; Carbonio, R.E.; Reguera, E.

    2015-10-15

    This contribution discusses the intercalation of imidazole and its 2-ethyl derivative, and pyridine in 2D copper nitroprusside. In the interlayer region, neighboring molecules remain interacting throu gh their dipole and quadrupole moments, which supports the solid 3D crystal structure. The crystal structure of this series of intercalation compounds was solved and refined from powder X-ray diffraction patterns complemented with spectroscopic information. The intermolecular interactions were studied from the refined crystal structures and low temperature magnetic measurements. Due to strong attractive forces between neighboring molecules, the resulting π–π cloud overlapping enables the ferromagnetic coupling between metal centers on neighboring layers, which was actually observed for the solids containing imidazole and pyridine as intercalated molecules. For these two solids, the magnetic data were properly described with a model of six neighbors. For the solid containing 2-ethylimidazole and for 2D copper nitroprusside, a model of four neighbors in a plane is sufficient to obtain a reliable data fitting. - Highlights: • Intercalation of organic molecules in 2D copper (II) nitroprusside. • Molecular properties of intercalation compounds of 2D copper (II) nitroprusside. • Magnetic properties of hybrid inorganic–organic solids. • Hybrid inorganic–organic 3D framework.

  1. Micro-channel catalytic reactor integration in CAPER and research/development on highly tritiated water handling and processing

    SciTech Connect (OSTI)

    Demange, D.; Cristescu, I.; Fanghaenel, E.; Gramlich, N.; Le, T.L.; Michling, R.; Moosmann, H.; Simon, K.H.; Wagner, R.; Welte, S.; Glugla, M.; Shu, W.M.; Willms, R.S.

    2015-03-15

    The CAPER facility of the Tritium Laboratory Karlsruhe has demonstrated the technology for the tokamak exhaust processing. CAPER has been significantly upgraded to pursue research/development programs towards highly tritiated water (HTW) handling and processing. The preliminary tests using a metal oxide reactor producing HTW afterward de-tritiated with PERMCAT were successful. In a later stage, a micro-channel catalytic reactor was installed in view of long term research program on HTW. The integration of this new system in CAPER was carried out along with a careful safety analysis due to high risk associated with such experiments. First experiments using the μ-CCR were performed trouble free, and HTW up to 360 kCi/kg was produced at a rate of 0.5 g/h. Such HTW was collected into a platinum zeolite bed (2 g of HTW for 20 g of Pt-zeolite), and in-situ detritiation was performed via isotopic exchange with deuterium. These first experimental results with tritium confirmed the potential for the capture and exchange method to be used for HTW in ITER. (authors)

  2. Assessment of Ports for Offshore Wind Development in the United States

    SciTech Connect (OSTI)

    Elkinton, Chris; Blatiak, Alicia; Ameen, Hafsa

    2014-03-21

    As offshore wind energy develops in the United States, port facilities will become strategic hubs in the offshore wind farm supply chain because all plant and transport logistics must transit through these facilities. Therefore, these facilities must provide suitable infrastructure to meet the specific requirements of the offshore wind industry. As a result, it is crucial that federal and state policy-makers and port authorities take effective action to position ports in the offshore wind value chain to take best advantage of their economic potential. The U.S. Department of Energy tasked the independent consultancy GL Garrad Hassan (GL GH) with carrying out a review of the current capability of U.S. ports to support offshore wind project development and an assessment of the challenges and opportunities related to upgrading this capability to support the growth of as many as 54 gigawatts of offshore wind installed in U.S. waters by 2030. The GL GH report and the open-access web-based Ports Assessment Tool resulting from this study will aid decision-makers in making informed decisions regarding the choice of ports for specific offshore projects, and the types of investments that would be required to make individual port facilities suitable to serve offshore wind manufacturing, installation and/or operations. The offshore wind industry in the United States is still in its infancy and this study finds that additional port facilities capable of supporting offshore wind projects are needed to meet the anticipated project build-out by 2030; however, no significant barriers exist to prevent the development of such facilities. Furthermore, significant port capabilities are in place today with purpose-build port infrastructure currently being built. While there are currently no offshore wind farms operating in the United States, much of the infrastructure critical to the success of such projects does exist, albeit in the service of other industries. This conclusion is based

  3. Two types of the relation between the intensity and the life time of photoluminescence of core/shell semiconductor quantum dots: Important role of Coulomb field and tunneling transitions

    SciTech Connect (OSTI)

    Osad'ko, I. S.

    2014-10-28

    It has been recently found [Gh. Galland, Y. Ghosh, A. Steinbrück, M. Sykora, J. A. Hollingsworth, and V. I. Klimov, Nature (London) 479, 203 (2011)] that semiconductor core/shell nanocrystals (NCs) with blinking photoluminescence (PL) can be of “A” or “B” type. NCs of A-type exhibit correlation between the intensity of PL and the life time. In NCs of B-type such correlation is absent. Simple model based on combination of the charging model and the two-level system model is proposed for describing emissive properties of NCs of both types. The model invokes fluctuations of emission ability γ{sub em} of NC to explain the emissive properties of NCs of B-type. Our combined model is also in agreement with anticorrelation between the duration τ{sub off} of off intervals and PL life time t{sub off} in off intervals found recently for NCs of A-type in the experiment [A. A. Cordones, T. J. Bixby, and S. R. Leone, Nano Lett. 11, 3366 (2011)].

  4. Lignocellulose Degradation Mechanisms Across the Tree of Life

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Cragg, Simon M.; Beckham, Gregg T.; Bruce, Neil C.; Bugg, Timothy D. H.; Distel, Daniel L.; Dupree, Paul; Etxabe, Amaia Green; Goodell, Barry S.; Jellison, Jody; McGeehan, John E.; et al

    2015-11-14

    Organisms use diverse mechanisms involving multiple complementary enzymes, particularly glycoside hydrolases (GHs), to deconstruct lignocellulose. Lytic polysaccharide monooxygenases (LPMOs) produced by bacteria and fungi facilitate deconstruction as does the Fenton chemistry of brown-rot fungi. Lignin depolymerisation is achieved by white-rot fungi and certain bacteria, using peroxidases and laccases. Meta-omics is now revealing the complexity of prokaryotic degradative activity in lignocellulose-rich environments. Protists from termite guts and some oomycetes produce multiple lignocellulolytic enzymes. We found that the Lignocellulose-consuming animals secrete some GHs, but most harbour a diverse enzyme-secreting gut microflora in a mutualism that is particularly complex in termites. Shipworms however,more » house GH-secreting and LPMO-secreting bacteria separate from the site of digestion and the isopod Limnoria relies on endogenous enzymes alone. Moreover, the omics revolution is identifying many novel enzymes and paradigms for biomass deconstruction, but more emphasis on function is required, particularly for enzyme cocktails, in which LPMOs may play an important role.« less

  5. Understanding Free and Complexed Enzyme Mechanisms and Factors Contributing to Cell Wall Recalcitrance (Presentation)

    SciTech Connect (OSTI)

    Resch, M.; Donohoe, B.; Katahira, R.; Ashutosh, M.; Beckham, G.; Himmel, M.; Decker, S.

    2014-04-01

    Fungal free enzymes and bacterial complexed cellulosomes deconstruct biomass using different physical mechanisms. Free enzymes, which typically contain a large proportion of GH7 cellobiohydrolase, diffuse throughout the substrate and hydrolyze primarily from the cellulose reducing end, resulting in 'sharpened' macrofibrils. In contrast, complexed cellulosomes contain a diverse array of carbohydrate binding modules and multiple catalytic specificities leading to delamination and physical peeling of the cellulose macrofibril structures. To investigate how cellulose structure contributes to recalcitrance, we compared the deconstruction of cellulose I, II, and III; using free and complexed enzyme systems. We also evaluated both systems on Clean Fractionation and alkaline pretreated biomass, which remove much of the lignin, to determine the impact on enzyme loading reduction. Free fungal enzymes demonstrated a swelling of the outer surface of the plant cell walls while removing localized disruptions, resulting in a smooth surface appearance. Cellulosomes produced cell wall surfaces with localized areas of disruption and little surface layer swelling. These studies contribute to the overall understanding of biomass recalcitrance and how combining different enzymatic paradigms may lead to the formulation of new enzyme cocktails to reduce the cost of producing sugars from plant cell wall carbohydrates.

  6. Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop

    SciTech Connect (OSTI)

    Godoy-Vitorino, Filipa; Malfatti, Stephanie; Garcia-Amado, Maria A.; Dominguez-Bello, Maria Gloria; Hugenholtz, Phillip; Tringe, Susannah

    2011-05-31

    The hoatzin (Opisthocomus hoazin) is a South American herbivorous bird, that has an enlarged crop analogous to the rumen, where foregut microbes degrade the otherwise indigestible plant materials, providing energy to the host. The crop harbors an impressive array of microorganisms with potentially novel cellulolytic enzymes. Thie study describes the composition ofthe particle-associated microbiota in the hoatzin crop, combining a survey of 16S rRNA genes in 7 adult birds and metagenome sequencing of two animals. The pyrotag survey demonstrates that Prevotellaceae, are the most abundant and ubiquitous taxa, suggesting that the degradation of hemicellulose is an important activity in the crop. Nonetheless, preliminary results from the metagnome of the particle-associated microbiota of two adult birds show that the crop microbiome contains a high number of genes encoding cellulases (such as GH5) more abundant than those of the termite gut, as well as genes encoding hemicellulases. These preliminary results show that the carbohydate-active enzyme genes in the cropmetagenome could be a source of biochemical catalysts able to deconstruct plant biomass.

  7. Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum 27405 Cellulosome Composition- a Quantitative Proteomic Analysis

    SciTech Connect (OSTI)

    Raman, Babu; Pan, Chongle; Hurst, Gregory {Greg} B; Rodriguez, Jr., Miguel; McKeown, Catherine K; Lankford, Patricia K; Samatova, Nagiza F; Mielenz, Jonathan R

    2009-01-01

    The anaerobic thermophilic bacterium Clostridium thermocellum is a cellulolytic organism capable of hydrolyzing cellulose and fermenting the hydrolysis products to ethanol and other metabolic products. C. thermocellum achieves efficient cellulose hydrolysis using multiprotein extracellular enzymatic complexes, termed the cellulosomes. In this study, we used quantitative proteomics (multidimensional LC-MS/MS and 15N-metabolic labeling) to measure relative changes in levels of cellulosomal subunit proteins (per CipA scaffoldin basis) when C. thermocellum was grown on a variety of carbon sources [dilute-acid pretreated switchgrass, cellobiose, amorphous cellulose, crystalline cellulose (Avicel) and combinations of crystalline cellulose with pectin or xylan or both]. Cellulosome samples isolated from cultures grown on these carbon sources were compared to 15N labeled cellulosome samples isolated from crystalline cellulose grown cultures. In total from all samples, proteomic analysis identified 59 dockerin- and 8 cohesin-module containing components, including 15 previously undetected cellulosomal subunits. Many cellulosomal components showed differential protein abundance in the presence of non-cellulose substrates in the growth medium. Cellulosome samples from amorphous cellulose, cellobiose and pretreated switchgrass grown cultures displayed the most distinct differences in composition as compared to cellulosome samples from crystalline cellulose grown cultures. While Glycoside Hydrolase Family 9 enzymes showed increased levels in the presence of crystalline cellulose, and pretreated switchgrass in particular, GH5 enzymes showed increased levels in response to the presence of cellulose in general, amorphous or crystalline. Overall, the results suggest a coordinated substrate-specific regulation of cellulosomal composition in C. thermocellum.

  8. Suite of Activity-Based Probes for Cellulose-Degrading Enzymes

    SciTech Connect (OSTI)

    Chauvigne-Hines, Lacie M.; Anderson, Lindsey N.; Weaver, Holly M.; Brown, Joseph N.; Koech, Phillip K.; Nicora, Carrie D.; Hofstad, Beth A.; Smith, Richard D.; Wilkins, Michael J.; Callister, Stephen J.; Wright, Aaron T.

    2012-12-19

    Microbial glycoside hydrolases play a dominant role in the biochemical conversion of cellulosic biomass to high-value biofuels. Anaerobic cellulolytic bacteria are capable of producing multicomplex catalytic subunits containing cell-adherent cellulases, hemicellulases, xylanases, and other glycoside hydrolases to facilitate the degradation of highly recalcitrant cellulose and other related plant cell wall polysaccharides. Clostridium thermocellum is a cellulosome producing bacterium that couples rapid reproduction rates to highly efficient degradation of crystalline cellulose. Herein, we have developed and applied a suite of difluoromethylphenyl aglycone, N-halogenated glycosylamine, and 2-deoxy-2-fluoroglycoside activity-based protein profiling (ABPP) probes to the direct labeling of the C. thermocellum cellulosomal secretome. These activity-based probes (ABPs) were synthesized with alkynes to harness the utility and multimodal possibilities of click chemistry, and to increase enzyme active site inclusion for LC-MS analysis. We directly analyzed ABP-labeled and unlabeled global MS data, revealing ABP selectivity for glycoside hydrolase (GH) enzymes in addition to a large collection of integral cellulosome-containing proteins. By identifying reactivity and selectivity profiles for each ABP, we demonstrate our ability to widely profile the functional cellulose degrading machinery of the bacterium. Derivatization of the ABPs, including reactive groups, acetylation of the glycoside binding groups, and mono- and disaccharide binding groups, resulted in considerable variability in protein labeling. Our probe suite is applicable to aerobic and anaerobic cellulose degrading systems, and facilitates a greater understanding of the organismal role associated within biofuel development.

  9. Lignocellulose Degradation Mechanisms Across the Tree of Life

    SciTech Connect (OSTI)

    Cragg, Simon M.; Beckham, Gregg T.; Bruce, Neil C.; Bugg, Timothy D. H.; Distel, Daniel L.; Dupree, Paul; Etxabe, Amaia Green; Goodell, Barry S.; Jellison, Jody; McGeehan, John E.; McQueen-Mason, Simon J.; Schnorr, Kirk; Walton, Paul H.; Watts, Joy E. M.; Zimmer, Martin

    2015-11-14

    Organisms use diverse mechanisms involving multiple complementary enzymes, particularly glycoside hydrolases (GHs), to deconstruct lignocellulose. Lytic polysaccharide monooxygenases (LPMOs) produced by bacteria and fungi facilitate deconstruction as does the Fenton chemistry of brown-rot fungi. Lignin depolymerisation is achieved by white-rot fungi and certain bacteria, using peroxidases and laccases. Meta-omics is now revealing the complexity of prokaryotic degradative activity in lignocellulose-rich environments. Protists from termite guts and some oomycetes produce multiple lignocellulolytic enzymes. We found that the Lignocellulose-consuming animals secrete some GHs, but most harbour a diverse enzyme-secreting gut microflora in a mutualism that is particularly complex in termites. Shipworms however, house GH-secreting and LPMO-secreting bacteria separate from the site of digestion and the isopod Limnoria relies on endogenous enzymes alone. Moreover, the omics revolution is identifying many novel enzymes and paradigms for biomass deconstruction, but more emphasis on function is required, particularly for enzyme cocktails, in which LPMOs may play an important role.

  10. Coulomb wave functions in momentum space

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Eremenko, V; Upadhyay, N. J.; Thompson, I J; Elster, Charlotte; Nunes, F. M.; Arbanas, Goran; Escher, J.E.; Hlophe, L.

    2015-01-01

    An algorithm to calculate non-relativistic partial-wave Coulomb functions in momentum space is presented. The arguments are the Sommerfeld parameter eta, the angular momentum l, the asymptotic momentum q and the 'running' momentum p, where both momenta are real. Since the partial-wave Coulomb functions exhibit singular behavior when p -> q, different representations of the Legendre functions of the 2nd kind need to be implemented in computing the functions for the values of p close to the singularity and far away from it. The code for the momentum-space Coulomb wave functions is applicable for values of vertical bar eta vertical barmore » in the range of 10(-1) to 10, and thus is particularly suited for momentum space calculations of nuclear reactions. Program Summary Program title: libcwfn Catalogue identifier: AEUQ_v1_0 Program summary URL: http://cpc.cs.qub.ac.uk/summaries/AEUQ_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 864503 No. of bytes in distributed program, including test data, etc.: 7178021 Distribution format: tar.gz Programming language: Fortran 90, Fortran 77, Python, make (GNU Make dialect), GNU Bash shell interpreter (available as /bin/bash). Computer: Apple Powermac (Intel Xeon), ASUS K53U (AMD E-350 (Dual Core)), DELL Precision T3500 (Intel Xeon), NERSC Carver (Intel Nehalem Quad Core). Operating system: Linux, Windows (using Cygwin). RAM: less than 512 Mbytes Classification: 17.8, 17.13, 17.16. Nature of problem: The calculation of partial wave Coulomb functions with integer land all other arguments real. Solution method: Computing the value of the function using explicit formulae and algorithms. Running time: Less than 10(-3) s. (C) 2014 Elsevier B.V. All rights reserved.« less

  11. Disequilibrium study of natural radionuclides of uranium and thorium series in cores and briny groundwaters from Palo Duro Basin, Texas

    SciTech Connect (OSTI)

    Laul, J.C.; Smith, M.R.

    1988-05-01

    The concentrations of natural radionuclides of the /sup 238/U and /232/Th series are reported in several cores and in ten deep and five shallow briny groundwaters from various formations in the Palo Duro Basin. The formations include Granite Wash, Pennsylvanian Granite Wash, Wolfcamp Carbonate, Pennsylvanian Carbonate, Seven River, Queen Grayburg, San Andres, Yates and Salado. The natural radionuclide data in cores suggest that the radionuclides have not migrated or been leached for at least a period of about 1 million years. Relative to the U and Th concentrations in cores, the brines are depleted by a factor of 10/sup 4/ to 10/sup 5/, indicating extremely low solubility of U and Th in brines. The natural radionuclide data in brines suggest that radium is not sorbed significantly and thus not retarded in nine deep brines. Radium is somewhat sorbed in one deep brine of Wolfcamp Carbonate and significantly sorbed in shallow brines. Relative to radium, the U, Th, Pb, Bi, and Po radionuclides are highly retarded by sorption. The retardation factors for /sup 228/Th range from 10/sup 2/ to 10/sup 3/, whereas those for /sup 230/Th and /sup 234/U range from 10/sup 3/ to 10/sup 5/, depending on the formation. The /sup 234/U//sup 238/U ratios in these brines are constant at about 1.5. The magnitude of the /sup 234/U//sup 230/Th ratio appears to reflect the degree of redox state of the aquifer's environment. The /sup 234/U//sup 230/Th ratio in nine deep brines is about unity, suggesting that U, like Th/sup +4/, is in the +4 state, which in turn suggests a reduced environment. 49 refs., 23 figs., 18 tabs.

  12. Integration of advanced geoscience and engineering techniques to quantify interwell heterogeneity in reservoir models. Final report, September 29, 1993--September 30, 1996

    SciTech Connect (OSTI)

    Weiss, W.W.; Buckley, J.S.; Ouenes, A.

    1997-05-01

    The goal of this three-year project was to provide a quantitative definition of reservoir heterogeneity. This objective was accomplished through the integration of geologic, geophysical, and engineering databases into a multi-disciplinary understanding of reservoir architecture and associated fluid-rock and fluid-fluid interactions. This interdisciplinary effort integrated geological and geophysical data with engineering and petrophysical results through reservoir simulation to quantify reservoir architecture and the dynamics of fluid-rock and fluid-fluid interactions. An improved reservoir description allows greater accuracy and confidence during simulation and modeling as steps toward gaining greater recovery efficiency from existing reservoirs. A field laboratory, the Sulimar Queen Unit, was available for the field research. Several members of the PRRC staff participated in the development of improved reservoir description by integration of the field and laboratory data as well as in the development of quantitative reservoir models to aid performance predictions. Subcontractors from Stanford University and the University of Texas at Austin (UT) collaborated in the research and participated in the design and interpretation of field tests. The three-year project was initiated in September 1993 and led to the development and application of various reservoir description methodologies. A new approach for visualizing production data graphically was developed and implemented on the Internet. Using production data and old gamma rays logs, a black oil reservoir model that honors both primary and secondary performance was developed. The old gamma ray logs were used after applying a resealing technique, which was crucial for the success of the project. In addition to the gamma ray logs, the development of the reservoir model benefitted from an inverse Drill Stem Test (DST) technique which provided initial estimates of the reservoir permeability at different wells.

  13. Petroleum prospects for offshore sedimentary basins in the eastern Papua New Guinea and Solomon Islands regions

    SciTech Connect (OSTI)

    Bruns, T.R.; Vedder, J.G. )

    1990-06-01

    Intra-arc basins in the Buka-Bougainville region of Papua New Guinea and in the Solomon Islands contain thick sedimentary sequences that may be prospective for petroleum. The Queen Emma basin, between Bougainville and New Ireland, contains as much as 8 km of deformed Oligocene and younger strata. The Central Solomons Trough, which underlies New Georgia Sound, is a composite intra-arc basin that contains late Oligocene and younger strata as much as 7 km thick. Farther east, beneath Indispensable Strait, the down-faulted Indispensable basin locally contains as much as 5.4 km of Miocene( ) and younger strata, and the offshore part of Mbokokimbo basin off eastern Guadalcanal includes 6 km or more of late Miocene and younger strata. All of these basins have some of the attributes necessary to generate and trap petroleum. Structural and stratigraphic traps are common, including faulted anticlines, sedimentary wedges, and carbonate reefs and reef-derived deposits on submarine ridges and along the basin margins. The thickness of the basin deposits ensures that some strata are buried deeply enough to be within the thermal regime required for hydrocarbon generation. However, little source or reservoir rock information is available because of the lack of detailed surface and subsurface stratigraphy. Moreover, much of the basin sediment is likely to consist of volcaniclastic material, derived from uplifted volcanogenic rocks surrounding the basins, and may be poor in source and reservoir rocks. Until additional stratigraphic information is available, analysis of the petroleum potential of these basins is a matter of conjecture.

  14. Report on the third international workshop on chromosome 9

    SciTech Connect (OSTI)

    Povey, S.; White, J.A.; Armour, J.

    1994-04-01

    The Third International Workshop on human chromosome 9 was held at Queens` College, Cambridge 9-11 April, 1994. The meeting was attended by 74 participants from 12 countries. On the morning of 12 April a satellite meeting was held on Tuberous Sclerosis, and because of its relevance to chromosome 9 a summary of this meeting is also presented within this report. The division consisted of a group with global interests, four regional groups 9p, 9q11-q21, 9q22-q33 and 9q33-qter, a group interested in mapping putative suppressor genes in ovarian and bladder cancer and a comparative mapping group. There was also discussion of resources, both physical and informatic. The amount of information on chromosome 9 has increased greatly in the past two years and it is clear that the integration of different types of information and the display of such information is an urgent problem. At this meeting two possible systems were explored, SIGMA and 1db. As described in the global group report an attempt was made to enter all mapping information into SIGMA, a program developed by Michael Cinkosky at Los Alamos. Within the text of this report a name without a date refers to an abstract at this meeting. A name with a date refers to a publication listed in the references and these are in general confined to very recent or {open_quote}in press{close_quote} references. A verbal communication at the meeting is identified as a personal communication. For authoritative referencing of published information and also for all primer sequences (except a few actually listed in the abstracts) the reader should consult GDB. The proceedings of the two previous workshops have been published and are listed in the references. It was decided that a fourth workshop would be held in about a year`s time. Dr. Margaret Pericak-Vance offered to host this at Duke University, North Carolina.

  15. Coulomb wave functions in momentum space

    SciTech Connect (OSTI)

    Eremenko, V; Upadhyay, N. J.; Thompson, I J; Elster, Charlotte; Nunes, F. M.; Arbanas, Goran; Escher, J.E.; Hlophe, L.

    2015-01-01

    An algorithm to calculate non-relativistic partial-wave Coulomb functions in momentum space is presented. The arguments are the Sommerfeld parameter eta, the angular momentum l, the asymptotic momentum q and the 'running' momentum p, where both momenta are real. Since the partial-wave Coulomb functions exhibit singular behavior when p -> q, different representations of the Legendre functions of the 2nd kind need to be implemented in computing the functions for the values of p close to the singularity and far away from it. The code for the momentum-space Coulomb wave functions is applicable for values of vertical bar eta vertical bar in the range of 10(-1) to 10, and thus is particularly suited for momentum space calculations of nuclear reactions. Program Summary Program title: libcwfn Catalogue identifier: AEUQ_v1_0 Program summary URL: http://cpc.cs.qub.ac.uk/summaries/AEUQ_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 864503 No. of bytes in distributed program, including test data, etc.: 7178021 Distribution format: tar.gz Programming language: Fortran 90, Fortran 77, Python, make (GNU Make dialect), GNU Bash shell interpreter (available as /bin/bash). Computer: Apple Powermac (Intel Xeon), ASUS K53U (AMD E-350 (Dual Core)), DELL Precision T3500 (Intel Xeon), NERSC Carver (Intel Nehalem Quad Core). Operating system: Linux, Windows (using Cygwin). RAM: less than 512 Mbytes Classification: 17.8, 17.13, 17.16. Nature of problem: The calculation of partial wave Coulomb functions with integer land all other arguments real. Solution method: Computing the value of the function using explicit formulae and algorithms. Running time: Less than 10(-3) s. (C) 2014 Elsevier B.V. All rights reserved.

  16. A survey of spatially distributed exterior dust lead loadings in New York City

    SciTech Connect (OSTI)

    Caravanos, Jack; Weiss, Arlene L.; Blaise, Marc J.; Jaeger, Rudolph J. . E-mail: jaegerr@envmed.com

    2006-02-15

    This work documents ambient lead dust deposition values (lead loading) for the boroughs of New York City in 2003-2004. Currently, no regulatory standards exist for exterior concentrations of lead in settled dust. This is in contrast to the clearance and risk assessment standards that exist for interior residential dust. The reported potential for neurobehavioral toxicity and adverse cognitive development in children due to lead exposure prompts public health concerns about undocumented lead sources. Such sources may include settled dust of outdoor origin. Dust sampling throughout the five boroughs of NYC was done from the top horizontal portion of pedestrian traffic control signals (PTCS) at selected street intersections along main thoroughfares. The data (n=214 samples) show that lead in dust varies within each borough with Brooklyn having the highest median concentration (730{mu}g/ft{sup 2}), followed in descending order by Staten Island (452{mu}g/ft{sup 2}), the Bronx (382{mu}g/ft{sup 2}), Queens (198{mu}g/ft{sup 2}) and finally, Manhattan (175{mu}g/ft{sup 2}). When compared to the HUD/EPA indoor lead in dust standard of 40{mu}g/ft{sup 2}, our data show that this value is exceeded in 86% of the samples taken. An effort was made to determine the source of the lead in the dust atop of the PTCS. The lead in the dust and the yellow signage paint (which contains lead) were compared using isotopic ratio analysis. Results showed that the lead-based paint chip samples from intact signage did not isotopically match the dust wipe samples taken from the same surface. We know that exterior dust containing lead contributes to interior dust lead loading. Therefore, settled leaded dust in the outdoor environment poses a risk for lead exposure to children living in urban areas, namely, areas with elevated childhood blood lead levels and background lead dust levels from a variety of unidentified sources.

  17. Measuring solar reflectance Part I: Defining a metric that accurately predicts solar heat gain

    SciTech Connect (OSTI)

    Levinson, Ronnen; Akbari, Hashem; Berdahl, Paul

    2010-05-14

    Solar reflectance can vary with the spectral and angular distributions of incident sunlight, which in turn depend on surface orientation, solar position and atmospheric conditions. A widely used solar reflectance metric based on the ASTM Standard E891 beam-normal solar spectral irradiance underestimates the solar heat gain of a spectrally selective 'cool colored' surface because this irradiance contains a greater fraction of near-infrared light than typically found in ordinary (unconcentrated) global sunlight. At mainland U.S. latitudes, this metric RE891BN can underestimate the annual peak solar heat gain of a typical roof or pavement (slope {le} 5:12 [23{sup o}]) by as much as 89 W m{sup -2}, and underestimate its peak surface temperature by up to 5 K. Using R{sub E891BN} to characterize roofs in a building energy simulation can exaggerate the economic value N of annual cool-roof net energy savings by as much as 23%. We define clear-sky air mass one global horizontal ('AM1GH') solar reflectance R{sub g,0}, a simple and easily measured property that more accurately predicts solar heat gain. R{sub g,0} predicts the annual peak solar heat gain of a roof or pavement to within 2 W m{sup -2}, and overestimates N by no more than 3%. R{sub g,0} is well suited to rating the solar reflectances of roofs, pavements and walls. We show in Part II that R{sub g,0} can be easily and accurately measured with a pyranometer, a solar spectrophotometer or version 6 of the Solar Spectrum Reflectometer.

  18. Toward Molecular Catalysts by Computer

    SciTech Connect (OSTI)

    Raugei, Simone; DuBois, Daniel L.; Rousseau, Roger J.; Chen, Shentan; Ho, Ming-Hsun; Bullock, R. Morris; Dupuis, Michel

    2015-02-17

    Rational design of molecular catalysts requires a systematic approach to designing ligands with specific functionality and precisely tailored electronic and steric properties. It then becomes possible to devise computer protocols to predict accurately the required properties and ultimately to design catalysts by computer. In this account we first review how thermodynamic properties such as oxidation-reduction potentials (E0), acidities (pKa), and hydride donor abilities (ΔGH-) form the basis for a systematic design of molecular catalysts for reactions that are critical for a secure energy future (hydrogen evolution and oxidation, oxygen and nitrogen reduction, and carbon dioxide reduction). We highlight how density functional theory allows us to determine and predict these properties within “chemical” accuracy (~ 0.06 eV for redox potentials, ~ 1 pKa unit for pKa values, and ~ 1.5 kcal/mol for hydricities). These quantities determine free energy maps and profiles associated with catalytic cycles, i.e. the relative energies of intermediates, and help us distinguish between desirable and high-energy pathways and mechanisms. Good catalysts have flat profiles that avoid high activation barriers due to low and high energy intermediates. We illustrate how the criterion of a flat energy profile lends itself to the prediction of design points by computer for optimum catalysts. This research was carried out in the Center for Molecular Electro-catalysis, an Energy Frontier Research Center funded by the U.S. Department of Energy (DOE), Office of Science, Office of Basic Energy Sciences. Pacific Northwest National Laboratory (PNNL) is operated for the DOE by Battelle.

  19. Specific uptake, dissociation, and degradation of /sup 125/I-labeled insulin in isolated turtle (Chrysemys dorbigni) thyroid glands

    SciTech Connect (OSTI)

    Marques, M.; da Silva, R.S.; Turyn, D.; Dellacha, J.M.

    1985-11-01

    Thyroid glands from turtles (Chrysemys dorbigni) pretreated with potassium iodide were incubated with /sup 125/I-insulin in the presence or absence of unlabeled insulin, in order to study its specific uptake. At 24 degrees, the specific uptake reached a plateau at 180 min of incubation. The dose of bovine insulin that inhibited 50% of the /sup 125/I-insulin uptake was 2 micrograms/ml of incubation medium. Most of the radioactive material (71%) extracted from the gland, after 30 min incubation with /sup 125/I-insulin, eluted in the same position as labeled insulin on Sephadex G-50. Only 24% eluted in the salt position. After 240 min incubation, increased amount of radioactivity appeared in the Na/sup 125/I position. When bovine insulin was added together with the labeled hormone, a substantial reduction of radioactivity was observed in the insulin and Na/sup 125/I elution positions. Dissociation studies were performed at 6 degrees in glands preincubated with /sup 125/I-insulin either at 24 or 6 degrees. The percentage of trichloroacetic acid (TCA)-soluble radioactive material in the dissociation medium increased with incubation time at both temperatures. However, the degradation activity was lower at 6 than at 24 degrees. The addition of bovine insulin to the incubation buffer containing /sup 125/I-insulin reduced the radioactive degradation products in the dissociated medium. Chloroquine or bacitracin inhibited the degradation activity. Incubation of thyroid glands with /sup 125/I-hGH or /sup 125/I-BSA showed values of uptake, dissociation, and degradation similar to those experiments in which an excess of bovine insulin was added together with the labeled hormone. Thus, by multiple criteria, such as specific uptake, dissociation, and degradation, the presence of insulin-binding sites in the turtle thyroid gland may be suggested.

  20. Evolution of high cellulolytic activity in symbiotic Streptomyces through selection of expanded gene content and coordinated gene expression

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Book, Adam J.; Lewin, Gina R.; McDonald, Bradon R.; Takasuka, Taichi E.; Wendt-Pienkowski, Evelyn; Doering, Drew T.; Suh, Steven; Raffa, Kenneth F.; Fox, Brian G.; Currie, Cameron R.

    2016-06-08

    In this study, the evolution of cellulose degradation was a defining event in the history of life. Without efficient decomposition and recycling, dead plant biomass would quickly accumulate and become inaccessible to terrestrial food webs and the global carbon cycle. On land, the primary drivers of plant biomass deconstruction are fungi and bacteria in the soil or associated with herbivorous eukaryotes. While the ecological importance of plant-decomposing microbes is well established, little is known about the distribution or evolution of cellulolytic activity in any bacterial genus. Here we show that in Streptomyces, a genus of Actinobacteria abundant in soil andmore » symbiotic niches, the ability to rapidly degrade cellulose is largely restricted to two clades of host-associated strains and is not a conserved characteristic of the Streptomyces genus or host-associated strains. Our comparative genomics identify that while plant biomass degrading genes (CAZy) are widespread in Streptomyces, key enzyme families are enriched in highly cellulolytic strains. Transcriptomic analyses demonstrate that cellulolytic strains express a suite of multi-domain CAZy enzymes that are coregulated by the CebR transcriptional regulator. Using targeted gene deletions, we verify the importance of a highly expressed cellulase (GH6 family cellobiohydrolase) and the CebR transcriptional repressor to the cellulolytic phenotype. Evolutionary analyses identify complex genomic modifications that drive plant biomass deconstruction in Streptomyces, including acquisition and selective retention of CAZy genes and transcriptional regulators. Our results suggest that host-associated niches have selected some symbiotic Streptomyces for increased cellulose degrading activity and that symbiotic bacteria are a rich biochemical and enzymatic resource for biotechnology.« less

  1. Reflection thermal diffuse x-ray scattering for quantitative determination of phonon dispersion relations

    SciTech Connect (OSTI)

    Mei, A. B.; Hellman, O.; Schlepuetz, C. M.; Rockett, A.; Chiang, T. -C.; Hultman, L.; Petrov, I.; Greene, J. E.

    2015-11-03

    Synchrotron reflection x-ray thermal diffuse scattering (TDS) measurements, rather than previously reported transmission TDS, are carried out at room temperature and analyzed using a formalism based upon second-order interatomic force constants and long-range Coulomb interactions to obtain quantitative determinations of MgO phonon dispersion relations (h) over bar omega(j) (q), phonon densities of states g((h) over bar omega), and isochoric temperature-dependent vibrational heat capacities cv (T). We use MgO as a model system for investigating reflection TDS due to its harmonic behavior as well as its mechanical and dynamic stability. Resulting phonon dispersion relations and densities of states are found to be in good agreement with independent reports from inelastic neutron and x-ray scattering experiments. Temperature-dependent isochoric heat capacities cv (T), computed within the harmonic approximation from (h) over bar omega(j) (q) values, increase with temperature from 0.4 x 10-4 eV/atom K at 100 K to 1.4 x 10-4 eV/atom K at 200 K and 1.9 x 10-4 eV/atom K at 300 K, in excellent agreement with isobaric heat capacity values cp (T) between 4 and 300 K. We anticipate that the experimental approach developed here will be valuable for determining vibrational properties of heteroepitaxial thin films since the use of grazing-incidence (θ ≲ θc where θc is the density-dependent critical angle) allows selective tuning of x-ray penetration depths to ≲ 10 nm.

  2. Reflection thermal diffuse x-ray scattering for quantitative determination of phonon dispersion relations

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Mei, A. B.; Hellman, O.; Schlepuetz, C. M.; Rockett, A.; Chiang, T. -C.; Hultman, L.; Petrov, I.; Greene, J. E.

    2015-11-03

    Synchrotron reflection x-ray thermal diffuse scattering (TDS) measurements, rather than previously reported transmission TDS, are carried out at room temperature and analyzed using a formalism based upon second-order interatomic force constants and long-range Coulomb interactions to obtain quantitative determinations of MgO phonon dispersion relations (h) over bar omega(j) (q), phonon densities of states g((h) over bar omega), and isochoric temperature-dependent vibrational heat capacities cv (T). We use MgO as a model system for investigating reflection TDS due to its harmonic behavior as well as its mechanical and dynamic stability. Resulting phonon dispersion relations and densities of states are found tomore » be in good agreement with independent reports from inelastic neutron and x-ray scattering experiments. Temperature-dependent isochoric heat capacities cv (T), computed within the harmonic approximation from (h) over bar omega(j) (q) values, increase with temperature from 0.4 x 10-4 eV/atom K at 100 K to 1.4 x 10-4 eV/atom K at 200 K and 1.9 x 10-4 eV/atom K at 300 K, in excellent agreement with isobaric heat capacity values cp (T) between 4 and 300 K. We anticipate that the experimental approach developed here will be valuable for determining vibrational properties of heteroepitaxial thin films since the use of grazing-incidence (θ ≲ θc where θc is the density-dependent critical angle) allows selective tuning of x-ray penetration depths to ≲ 10 nm.« less

  3. Alumina-Forming Austenitics: A New Approach to Thermal and Degradation Resistant Stainless Steels for Industrial Use

    SciTech Connect (OSTI)

    David A Helmick; John H Magee; Michael P Brady

    2012-05-31

    A series of developmental AFA alloys was selected for study based on: 25 Ni wt.% (alloys A-F), 20 wt% Ni (alloys G-H), and 12 Ni wt.% (alloys I-L). An emphasis in this work was placed on the lower alloy content direction for AFA alloys to reduce alloy raw material cost, rather than more highly alloyed and costly AFA alloys for higher temperature performance. Alloys A-D explored the effects of Al (3-4 wt.%) and C (0.05-0.2 wt.%) in the Fe-25Ni-14Cr-2Mn-2Mo-1W-1Nb wt.% base range; alloys E and F explored the effects of removing costly Mo and W additions in a Fe-25Ni-14Cr-4Al-2.5Nb-2Mn-0.2C base, alloys G and H examined Nb (1-2.5wt.%) and removal of Mo, W in a Fe-20Ni-14Cr-3Al-2Mn-0.2 C wt.% base; and alloys I-L examined effects of C (0.1-0.2 wt.%) and Mn (5-10 wt.%) on a low cost Fe-14Cr-12Ni-3Cu-2.5Al wt.% base (no Mo, W additions). Creep testing resulted in elemental trends that included the beneficial effect of higher carbon and lower niobium in 20-25%Ni AFA alloys and, the beneficial of lower Mn in 12%Ni AFA alloys. Corrosion tests in steam and sulfidation-oxidation environments showed, in general, these alloys were capable of a ten-fold improvement in performance when compared to conventional austenitic stainless steels. Also, corrosion test results in metal-dusting environments were promising and, warrant further investigation.

  4. CO2-Binding Organic Liquids Gas Capture with Polarity-Swing-Assisted Regeneration Full Technology Feasibility Study B1 - Solvent-based Systems

    SciTech Connect (OSTI)

    Heldebrant, David J

    2014-08-31

    PNNL, Fluor Corporation and Queens University (Kingston, ON) successfully completed a three year comprehensive study of the CO2BOL water-lean solvent platform with Polarity Swing Assisted Regeneration (PSAR). This study encompassed solvent synthesis, characterization, environmental toxicology, physical, thermodynamic and kinetic property measurements, Aspen Plus™ modeling and bench-scale testing of a candidate CO2BOL solvent molecule. Key Program Findings The key program findings are summarized as follows: • PSAR favorably reduced stripper duties and reboiler temperatures with little/no impact to absorption column • >90% CO2 capture was achievable at reasonable liquid-gas ratios in the absorber • High rich solvent viscosities (up to 600 cP) were successfully demonstrated in the bench-scale system. However, the projected impacts of high viscosity to capital cost and operational limits compromised the other levelized cost of electricity benefits. • Low thermal conductivity of organics significantly increased the required cross exchanger surface area, and potentially other heat exchange surfaces. • CO2BOL had low evaporative losses during bench-scale testing • There was no evidence of foaming during bench scale testing • Current CO2BOL formulation costs project to be $35/kg • Ecotoxicity (Water Daphnia) was comparable between CO2BOL and MEA (169.47 versus 103.63 mg/L) • Full dehydration of the flue gas was determined to not be economically feasible. However, modest refrigeration (13 MW for the 550 MW reference system) was determined to be potentially economically feasible, and still produce a water-lean condition for the CO2BOLs (5 wt% steady-state water loading). • CO2BOLs testing with 5 wt% water loading did not compromise anhydrous performance behavior, and showed actual enhancement of CO2 capture performance. • Mass transfer of CO2BOLs was not greatly impeded by viscosity • Facile separation of antisolvent from lean CO2BOL was

  5. Method of orbit sums in the theory of modular vector invariants

    SciTech Connect (OSTI)

    Stepanov, S A

    2006-12-31

    Let F be a field, V a finite-dimensional F-vector space, G{<=}GL{sub F}(V) a finite group, and V{sup m}=V+...+V the m-fold direct sum with the diagonal action of G. The group G acts naturally on the symmetric graded algebra A{sub m}=F[V{sup m}] as a group of non-degenerate linear transformations of the variables. Let A{sub m}{sup G} be the subalgebra of invariants of the polynomial algebra A{sub m} with respect to G. A classical result of Noether [1] says that if charF=0, then A{sub m}{sup G} is generated as an F-algebra by homogeneous polynomials of degree at most |G|, no matter how large m can be. On the other hand, it was proved by Richman [2], [3] that this result does not hold when the characteristic of F is positive and divides the order |G| of G. Let p, p>2, be a prime number, F=F{sub p} a finite field of p elements, V a linear F{sub p}-vector space of dimension n, and H{<=}GL{sub F{sub p}}(V) a cyclic group of order p generated by a matrix {gamma} of a certain special form. In this paper we describe explicitly (Theorem 1) one complete set of generators of A{sub m}{sup H}. After that, for an arbitrary complete set of generators of this algebra we find a lower bound for the highest degree of the generating elements of this algebra. This is a significant extension of the corresponding result of Campbell and Hughes [4] for the particular case of n=2. As a consequence we show (Theorem 3) that if m>n and G{>=}H is an arbitrary finite group, then each complete set of generators of A{sub m}{sup G} contains an element of degree at least 2(m-n+2r)(p-1)/r, where r=r(H) is a positive integer dependent on the structure of the generating matrix {gamma} of the group H. This result refines considerably the earlier lower bound obtained by Richman [3].

  6. Hydrogen production and delivery analysis in US markets : cost, energy and greenhouse gas emissions.

    SciTech Connect (OSTI)

    Mintz, M.; Gillette, J.; Elgowainy, A.

    2009-01-01

    and significant GHG emissions (in the absence of carbon caps, taxes or sequestration); (5) Nuclear pathway is most favorable from energy use and GHG emissions perspective; (6) GH2 Truck and Pipeline delivery have much lower energy use and GHG emissions than LH2 Truck delivery; and (7) For LH2 Truck delivery, the liquefier accounts for most of the energy and GHG emissions.

  7. Truckstop -- and Truck!-- Electrification

    SciTech Connect (OSTI)

    Skip Yeakel

    2001-12-13

    The conclusions of this paper are: 0.5-1.5 G/H and/or BUSG/Y--how much time and money will it take to quantify and WHY BOTHER TO DO SO? No shortage of things to do re truckstop--+ truck!-- electrification; Better that government and industry should put many eggs in lots of baskets vs. all in one or few; Best concepts will surface as most viable; Economic appeal better than regulation or brute force; Launch Ground Freight Partnership and give it a chance to work; Demonstration is an effective means to educate, and learn from, customers--learning is a two way street; Research, Development, Demonstration, and Deployment (RD 3) are all important but only deployment gets results; TSE can start small in numbers of spaces to accommodate economically inspired growth but upfront plans should be made for expansion if meaningful idle reduction is to follow via TE; 110VAC 15A service/ parking space is minimal--if infrastructure starts like this, upfront plans must be made to increase capacity; Increased electrification of truckstop and truck alike will result in much better life on the road; Improved sleep will improve driver alertness and safety; Reduced idling will significantly reduce fuel use and emissions; Universal appeal for DOD, DOE, DOT, EPA, OEMs, and users alike; Clean coal, gas, hydro, nuclear, or wind energy sources are all distinctly American means by which to generate electricity; Nothing can compete with diesel fuel to serve mobile truck needs; stationary trucks are like power plants--they don't move and should NOT be powered by petroleum products whenever possible; Use American fueled power plants--electricity--to serve truck idling needs wherever practical to do so; encourage economic aspect; Create and reward industry initiatives to reduce fuel use; Eliminate FET on new trucks, provide tax credits (non highway fuel use and investment), provide incentives based on results; Encourage newer/ cleaner truck use; solicit BAAs with mandatory OEM/ fleet

  8. A common region of deletion on chromosome 17q in both sporadic and familial epithelial ovarian tumors distal to BRCA1

    SciTech Connect (OSTI)

    Godwin, A.K.; Vanderveer, L.; Schultz, D.C.; Altomare, D.A.; Buetow, K.H.; Daly, M.; Getts, L.A.; Masny, A.; Rosenblum, N.

    1994-10-01

    Linkage analysis in familial breast and ovarian cancer and studies of allelic deletion in sporadic ovarian tumors have identified a region on chromosome 17q containing a candidate tumor-suppressor gene (referred to as BRCA1) of likely importance in ovarian carcinogenesis. We have examined normal and tumor DNA samples from 32 patients with sporadic and 8 patients with familial forms of the disease, for loss of heterozygosity (LOH) at 21 loci on chromosome 17 (7 on 17p and 14 on 17q). LOH on 17p was 55% (22/40) for informative 17p13.1 and 17p13.3 markers. When six polymorphic markers flanking the familial breast/ovarian cancer susceptibility locus on 17q12-q21 were used, LOH was 58% (23/40), with one tumor showing telomeric retention. Evaluation of a set of markers positioned telomeric to BRCA1 resulted in the highest degree of LOH, 73% (29/40), indicating that a candidate locus involved in ovarian cancer may reside distal to BRCA1. Five of the tumors demonstrating allelic loss for 17q markers were from individuals with a strong family history of breast and ovarian cancer. More important, two of these tumors (unique patient number [UPN] 57 and UPN 79) retained heterozygosity for all informative markers spanning the BRCA1 locus but showed LOH at loci distal to but not including the anonymous markers CMM86 (D17S74) and 42D6 (D17S588), respectively. Deletion mapping of seven cases (two familial and five sporadic) showing limited LOH on 17q revealed a common region of deletion, distal to GH and proximal to D17S4, that spans {approximately} 25 cM. These results suggest that a potential tumor-suppressor gene involved in both sporadic and familial ovarian cancer may reside on the distal portion of chromosome 17q and is distinct from the BRCA1 gene. 58 refs., 3 figs., 4 tabs.

  9. TH-C-18A-01: Is Automatic Tube Current Modulation Still Necessary with Statistical Iterative Reconstruction?

    SciTech Connect (OSTI)

    Li, K; Zhao, W; Gomez-Cardona, D; Chen, G

    2014-06-15

    Purpose: Automatic tube current modulation (TCM) has been widely used in modern multi-detector CT to reduce noise spatial nonuniformity and streaks to improve dose efficiency. With the advent of statistical iterative reconstruction (SIR), it is expected that the importance of TCM may diminish, since SIR incorporates statistical weighting factors to reduce the negative influence of photon-starved rays. The purpose of this work is to address the following questions: Does SIR offer the same benefits as TCM? If yes, are there still any clinical benefits to using TCM? Methods: An anthropomorphic CIRS chest phantom was scanned using a state-of-the-art clinical CT system equipped with an SIR engine (Veo, GE Healthcare). The phantom was first scanned with TCM using a routine protocol and a low-dose (LD) protocol. It was then scanned without TCM using the same protocols. For each acquisition, both FBP and Veo reconstructions were performed. All scans were repeated 50 times to generate an image ensemble from which noise spatial nonuniformity (NSN) and streak artifact levels were quantified. Monte-Carlo experiments were performed to estimate skin dose. Results: For FBP, noise streaks were reduced by 4% using TCM for both routine and LD scans. NSN values were actually slightly higher with TCM (0.25) than without TCM (0.24) for both routine and LD scans. In contrast, for Veo, noise streaks became negligible (<1%) with or without TCM for both routine and LD scans, and the NSN was reduced to 0.10 (low dose) or 0.08 (routine). The overall skin dose was 2% lower at the shoulders and more uniformly distributed across the skin without TCM. Conclusion: SIR without TCM offers superior reduction in noise nonuniformity and streaks relative to FBP with TCM. For some clinical applications in which skin dose may be a concern, SIR without TCM may be a better option. K. Li, W. Zhao, D. Gomez-Cardona: Nothing to disclose; G.-H. Chen: Research funded, General Electric Company Research funded

  10. Incorporating Amino Acid Esters into Catalysts for Hydrogen Oxidation: Steric and Electronic Effects and the Role of Water as a Base

    SciTech Connect (OSTI)

    Lense, Sheri; Ho, Ming-Hsun; Chen, Shentan; Jain, Avijita; Raugei, Simone; Linehan, John C.; Roberts, John A.; Appel, Aaron M.; Shaw, Wendy J.

    2012-10-08

    Four derivatives of a hydrogen oxidation catalyst, [Ni(PCy2NBn-R2)]2+ (Cy=cyclohexyl, Bn=benzyl, R= OMe, COOMe, CO-Alanine-methyl ester or CO-Phenylalanine-methyl ester), have been prepared to investigate steric and electronic effects on catalysis. Each complex was characterized spectroscopically and electrochemically, and thermodynamic data were determined. Crystal structures are also reported for the -OMe and -COOMe derivatives. All four catalysts were found to be active for H2 oxidation. The methyl ester (R = COOMe) and amino acid ester containing complexes (R = CO-Alanine-methyl ester or CO-Phenylalanine-methyl ester) had slower rates (4 s-1) than that of the parent complex (10 s-1), in which R = H, consistent with the lower amine pKa’s and less favorable GH2’s found for these electron-withdrawing substituents. Dynamic processes for the amino acid ester containing complexes were also investigated and found not to hinder catalysis. The electron-donating methoxy ether derivative (R = OMe) was prepared to compare electronic effects and has a similar catalytic rate as the parent complex. In the course of these studies, it was found that water could act as a weak base for H2 oxidation, although catalytic turnover requires a significantly higher potential and utilizes a different sequence of catalytic steps than when using a base with a higher pKa. Importantly, these catalysts provide a foundation upon which larger peptides can be attached to [Ni(PCy2NBn2)2]2+ hydrogen oxidation catalysts in order to more fully investigate and implement the effects of the outer-coordination sphere. This work was funded by the DOE Office of Science Early Career Research Program through the Office of Basic Energy Sciences (SL and WJS), by the Center for Molecular Electrocatalysis, an Energy Frontier Research Center funded by the US Department of Energy, Office of Science, Office of Basic Energy Sciences (JR), and by the US DOE Basic Energy Sciences, Chemical Sciences, Geoscience

  11. Ionic Liquids: Radiation Chemistry, Solvation Dynamics and Reactivity Patterns

    SciTech Connect (OSTI)

    Wishart,J.F.

    2008-09-29

    the influence of ILs on charge transport processes. Picosecond pulse radiolysis studies at BNL's Laser-Electron Accelerator Facility (LEAF) are used to identify reactive species in ionic liquids and measure their solvation and reaction rates. We and our collaborators (R. Engel (Queens College, CUNY) and S. Lall-Ramnarine, (Queensborough CC, CUNY)) develop and characterize new ionic liquids specifically designed for our radiolysis and solvation dynamics studies. IL solvation and rotational dynamics are measured by TCSPC and fluorescence upconversion measurements in the laboratory of E. W. Castner at Rutgers Univ. Investigations of radical species in irradiated ILs are carried out at ANL by I. Shkrob and S. Chemerisov using EPR spectroscopy. Diffusion rates are obtained by PGSE NMR in S. Greenbaum's lab at Hunter College, CUNY and S. Chung's lab at William Patterson U. Professor Mark Kobrak of CUNY Brooklyn College performs molecular dynamics simulations of solvation processes. A collaboration with M. Dietz at U. Wisc. Milwaukee is centered around the properties and radiolytic behavior of ionic liquids for nuclear separations. Collaborations with C. Reed (UC Riverside), D. Gabel (U. Bremen) and J. Davis (U. South Alabama) are aimed at characterizing the radiolytic and other properties of borated ionic liquids, which could be used to make fissile material separations processes inherently safe from criticality accidents.

  12. IONIC LIQUIDS: RADIATION CHEMISTRY, SOLVATION DYNAMICS AND REACTIVITY PATTERNS.

    SciTech Connect (OSTI)

    WISHART,J.F.

    2007-10-01

    's Laser-Electron Accelerator Facility (LEAF) are used to identify reactive species in ionic liquids and measure their solvation and reaction rates. We and our collaborators (R. Engel (Queens College, CUNY) and S. Lall-Ramnarine, (Queensborough CC, CUNY)) develop and characterize new ionic liquids specifically designed for our radiolysis and solvation dynamics studies. IL solvation and rotational dynamics are measured by TCSPC and fluorescence upconversion measurements in the laboratory of E. W. Castner at Rutgers Univ. Investigations of radical species in irradiated ILs are carried out at ANL by I. Shkrob and S. Chemerisov using EPR spectroscopy. Diffusion rates are obtained by PGSE NMR in S. Greenbaum's lab at Hunter College, CUNY and S. Chung's lab at William Patterson U. Professor Mark Kobrak of CUNY Brooklyn College performs molecular dynamics simulations of solvation processes. A collaboration with M. Dietz and coworkers at ANL is centered around the properties and radiolytic behavior of ionic liquids for nuclear separations. Collaborations with C. Reed (UC Riverside), D. Gabel (U. Bremen) and J. Davis (U. South Alabama) are aimed at characterizing the radiolytic and other properties of borated ionic liquids, which could be used to make fissile material separations processes inherently safe from criticality accidents.

  13. The age of the martian meteorite Northwest Africa 1195 and the differentiation history of the shergottites

    SciTech Connect (OSTI)

    Symes, S; Borg, L; Shearer, C; Irving, A

    2007-04-05

    Samarium-neodymium isotopic analyses of unleached and acid-leached mineral fractions from the recently identified olivine-bearing shergottite Northwest Africa 1195 yield a crystallization age of 348 {+-} 19 Ma and an {var_epsilon}{sub Nd}{sup 143} value of +40.1 {+-} 1.3. Maskelynite fractions do not lie on the Sm-Nd isochron and appear to contain a martian surface component with low {sup 147}Sm/{sup 144}Nd and {sup 143}Nd/{sup 144}Nd ratios that was added during shock. The Rb-Sr system is disturbed and does not yield an isochron. Terrestrial Sr appears to have affected all of the mineral fractions, although a maximum initial {sup 87}Sr/{sup 86}Sr ratio of 0.701614 {+-} 16 is estimated by passing a 348 Ma reference isochron through the maskelynite fraction that is least affected by contamination. The high initial {var_epsilon}{sub Nd}{sup 143} value and the low initial {sup 87}Sr/{sup 86}Sr ratio, combined with the geologically young crystallization age, indicate that Northwest Africa 1195 is derived from a source region characterized by a long-term incompatible element depletion. The age and initial Sr and Nd isotopic compositions of Northwest Africa 1195 are very similar to those of Queen Alexandra Range 94201, indicating these samples were derived from source regions with nearly identical Sr-Nd isotopic systematics. These similarities suggest that these two meteorites share a close petrogenetic relationship and might have been erupted from a common volcano. The meteorites Yamato 980459, Dar al Gani 476, Sayh al Uhaymir 005/008, and Dhofar 019 also have relatively old ages between 474-575 Ma and trace element and/or isotopic systematics that are indicative of derivation from incompatible-element-depleted sources. This suggests that the oldest group of meteorites is more closely related to one another than they are to the younger meteorites that are derived from less incompatible-element-depleted sources. Closed-system fractional crystallization of this suite of

  14. Milestones for Selection Characterization and Analysis of the Performance of a Repository for Spent Nuclear Fuel and HIh-Level Radioactive Waste at Yucca Mountain.

    SciTech Connect (OSTI)

    Rechard, Robert P.

    2015-02-01

    This report presents a concise history in tabular form of events leading up to site identification in 1978, site selection in 1987, subsequent characterization, and ongoing analysis throu gh 2009 of the performance of a repository for spent nuclear fuel and high - level radi oactive waste at Yucca Mou ntain in southern Nevada. The tabulated events generally occurred in five periods: (1) commitment to mined geologic disposal and identification of sites; (2) site selection and analysis, based on regional geologic characterization through literature and an alogous data; (3) feasibility analysis demonstrating calculation procedures and importance of system components, based on rough measures of performance using surface exploration, waste process knowledge, and general laboratory experiments; (4) suitability analysis demonstrating viability of disposal system, based on environment - specific laboratory experiments, in - situ experiments, and underground disposal system characterization; and (5) compliance analysis, based on completed site - specific characterization . The current sixth period beyond 2010 represents a new effort to set waste management policy in the United States. Because the relationship is important to understanding the evolution of the Yucca Mountain Project , the tabulation also shows the interaction between the policy realm and technical realm using four broad categories of events : (a ) R egulatory requirements and related federal policy in laws and court decisions, (c ) Presidential and agency directives, (c) technical milestones of implemen ting institutions, and (d ) critiques of the Yucca Mountai n P roject and pertinent national and world events related to nuclear energy and radioactive waste. Preface The historical progression of technical milestones for the Yucca Mountain Project was originally developed for 10 journal articles in a special issue of Reliability Engineering System Safe ty on the performance assessment for the Yucca Mountain

  15. CPsuperH2.3: an Updated Tool for Phenomenology in the MSSM with Explicit CP Violation

    SciTech Connect (OSTI)

    Lee, J.S.; Carena, M.; Ellis, J.; Pilaftsis, A.; Wagner, C.E.M.

    2013-04-01

    We describe the Fortran code CPsuperH2.3, which incorporates the following updates compared with its predecessor CPsuperH2.0. It implements improved calculations of the Higgs-boson masses and mixing including stau contributions and finite threshold effects on the tau-lepton Yukawa coupling. It incorporates the LEP limits on the processes e^+e^-->H_iZ,H_iH_j and the CMS limits on H_i->@t@?@t obtained from 4.6 fb^-^1 of data at a center-of-mass energy of 7 TeV. It also includes the decay mode H_i->Z@c and the Schiff-moment contributions to the electric dipole moments of Mercury and Radium 225, with several calculational options for the case of Mercury. These additions make CPsuperH2.3 a suitable tool for analyzing possible CP-violating effects in the MSSM in the era of the LHC and a new generation of EDM experiments. Program summary: Program title: CPsuperH2.3 Catalogue identifier: ADSR_v3_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/ADSR_v3_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 24058 No. of bytes in distributed program, including test data, etc.: 158721 Distribution format: tar.gz Programming language: Fortran77. Computer: PC running under Linux and computers in Unix environment. Operating system: Linux. RAM: 32 MB Classification: 11.1. Does the new version supersede the previous version?: Yes Catalogue identifier of previous version: ADSR_v2_0 Journal reference of previous version: Comput. Phys. Comm. 180(2009)312 Nature of problem: The calculations of mass spectrum, decay widths and branching ratios of the neutral and charged Higgs bosons in the Minimal Supersymmetric Standard Model with explicit CP violation have been improved. The program is based on renormalization-group-improved diagrammatic calculations that include dominant higher

  16. Tritium in the World Trade Center September 11, 2001 Terrorist Attack: It's Possible Sources and Fate

    SciTech Connect (OSTI)

    Parekh, P; Semkow, T; Husain, L; Haines, D; Woznial, G; Williams, P; Hafner, R; Rabun, R

    2002-05-03

    Traces of tritiated water (HTO) were determined at World Trade Center (WTC) ground zero after the 9/11/01 terrorist attack. A method of ultralow-background liquid scintillation counting was used after distilling HTO from the samples. A water sample from the WTC sewer, collected on 9/13/01, contained 0.174{plus_minus}0.074 (2{sigma}) nCi/L of HTO. A split water sample, collected on 9/21/01 from the basement of WTC Building 6, contained 3.53{plus_minus}0.17 and 2.83{plus_minus}0.15 nCi/L, respectively. Several water and vegetation samples were analyzed from areas outside the ground zero, located in Manhattan, Brooklyn, Queens, and Kensico Reservoir. No HTO above the background was found in those samples. All these results are well below the levels of concern to human exposure. Several tritium radioluminescent (RL) devices were investigated as possible sources of the traces of tritium at ground zero. Tritium is used in self-luminescent emergency EXIT signs. No such signs were present inside the WTC buildings. However, it was determined that Boeing 767-222 aircraft operated by the United Airlines that hit WTC Tower 2 as well as Boeing 767-223ER operated by the American Airlines, that hit WTC Tower 1, had a combined 34.3 Ci of tritium at the time of impact. Other possible sources of tritium include dials and lights of fire and emergency equipment, sights and scopes in weaponry, as well as time devices equipped with tritium dials. It was determined that emergency equipment was not a likely source. However, WTC hosted several law-enforcement agencies such as ATF, CIA, US Secret Service and US Customs. The ATF office had two weapon vaults in WTC Building 6. Also 63 Police Officers, possibly carrying handguns with tritium sights, died in the attack. The weaponry containing tritium was therefore a likely and significant source of tritium. It is possible that some of the 2830 victims carried tritium watches, however this source appears to be less significant that the other