National Library of Energy BETA

Sample records for quantitative trait loci

  1. Quantitative Trait Loci Analysis of Primary Cell Wall Composition in Arabidopsis1

    E-Print Network [OSTI]

    Pauly, Markus

    fingerprinting techniques: monosaccharide composition analysis by gas chromatography, xyloglucan oligosaccharideQuantitative Trait Loci Analysis of Primary Cell Wall Composition in Arabidopsis1 Gre´gory Mouille2 trait loci (QTL) analysis was used to identify genes underlying natural variation in primary cell wall

  2. Quantitative traits loci (QTL) involved in body colour, wing morphometry, cuticular hydrocarbons

    E-Print Network [OSTI]

    Quantitative traits loci (QTL) involved in body colour, wing morphometry, cuticular hydrocarbons for wing morphometry, 12 for cuticular hydrocarbons and five for venom components. We detected 50 QTL / cuticular hydrocarbons / venom 1. INTRODUCTION The first genetic map built from RAPD markers (Hunt and Page

  3. High resolution linkage and association study of quantitative trait loci 

    E-Print Network [OSTI]

    Jung, Jeesun

    2005-11-01

    allele marker, p1 = 0.50,k1 = k2 = 100; For a 3 allele marker, p1 = 0.4,p2 = 0.3,k1 = 100,k2 = k3 = 50; For a 4 allele marker, pi = 0.25,ki = 50,i = 1,???,4. . . . . . . . . . . . . . . . . . . . . . 24 4 Power curves of FGen Nuc,singleton,sibs for 2, 3... curves of FGen Nuc,singleton for 2, 3 and 4 allele markers against the recombination fraction at 0.05 significant level, when q1 = 0.25,?2G = 0.75,A = 20,h2 = 0.25 for a dominant trait a = d = 1.0, Graph I; and a recessive trait a = 1.0 and d = ?0...

  4. Developing a web accessible integrated database and visualization tool for bovine quantitative trait loci 

    E-Print Network [OSTI]

    Polineni, Pavana

    2005-08-29

    are sequence based. Map based browsers can be converted to sequence based browsers by anchoring marker reference ID to a sequence co- ordinate in a sequence database. The Genome Map Viewer (Davenport et al. 1999) is a Java graphical tool recently developed... by UK CropNet. The Genome Map Viewer can display elements such as loci and it has been used extensively for crop plant species. The GridMap (Priestly et al. 1999) is a generic grid-drawing Java tool that is particularly useful for the display...

  5. Copyright 2000 by the Genetics Society of America Quantitative Trait Loci for Floral Morphology in Arabidopsis thaliana

    E-Print Network [OSTI]

    Purugganan, Michael D.

    indicate that many of the iden- ORGANS (UFO)], influence the setting of boundaries tified loci that control floral development encode either between whorls [SUPERMAN (SUP) and UFO], and alter DNA

  6. Identification of Quantitative Trait Loci (QTLs) Associated with Maintenance of Bread Making Quality under Heat Stress in Wheat (Triticum aestivum) 

    E-Print Network [OSTI]

    Beecher, Francis Ward

    2010-10-12

    significant effects, particularly those associated with glutenin subunits or gliadins, are probably the best characterized. Blanco et al. (1998) detected a putative QTL for SDS (? = .01) associated with a Glu-A1 allele and another associated with the Gli-B1... of those identified thus far are on chromosomes 1A, 1B, and 1D (Blanco et al. 2002; MacRitchie and Lafiandra 2001). On the short arms of these chromosomes are found the complex Gli-1/Glu-3 loci coding for LMWGSs and ? and ? gliadins. The long arms...

  7. A powerful likelihood method for the analysis of linkage disequilibrium between trait loci and one or more polymorphic marker loci

    SciTech Connect (OSTI)

    Terwilliger, J.D. [Univ. of Oxford (United Kingdom)

    1995-03-01

    Historically, most methods for detecting linkage disequilibrium were designed for use with diallelic marker loci, for which the analysis is straightforward. With the advent of polymorphic markers with many alleles, the normal approach to their analysis has been either to extend the methodology for two-allele systems (leading to an increase in df and to a corresponding loss of power) or to select the allele believed to be associated and then collapse the other alleles, reducing, in a biased way, the locus to a diallelic system. I propose a likelihood-based approach to testing for linkage disequilibrium, an approach that becomes more conservative as the number of alleles increases, and as the number of markers considered jointly increases in a multipoint test for linkage disequilibrium, while maintaining high power. Properties of this method for detecting associations and fine mapping the location of disease traits are investigated. It is found to be, in general, more powerful than conventional methods, and it provides a tractable framework for the fine mapping of new disease loci. Application to the cystic fibrosis data of Kerem et al. is included to illustrate the method. 12 refs., 4 figs., 4 tabs.

  8. Brief Genetics Report Identification of Major Quantitative Trait Loci

    E-Print Network [OSTI]

    Yandell, Brian S.

    into the pathogenesis of human obesity. Diabetes 53:245­249, 2004 G enetic factors help determine an individual

  9. Bioinformatics tools for the genetic dissection of complex traits in chickens 

    E-Print Network [OSTI]

    Cabrera Cárdenas, Claudia Paola

    2009-01-01

    This thesis explores the genetic characterization of the mechanisms underlying complex traits in chicken through the use and development of bioinformatics tools. The characterization of quantitative trait loci controlling complex traits has proven...

  10. An experimental method for evaluating the contribution of deleterious mutations to quantitative trait variation

    E-Print Network [OSTI]

    Kelly, John K.

    1999-06-01

    Unconditionally deleterious mutations could be an important source of variation in quantitative traits. Deleterious mutations should be rare (segregating at low frequency in the population) and at least partially recessive. ...

  11. Heritability and Quantitative Trait Loci for Popping Characteristics in Sorghum Grain 

    E-Print Network [OSTI]

    Pugh, Nicholas Ace

    2015-08-12

    intolerance has spurred an interest in gluten free grains. Since sorghum is one such grain, it has gained market share where gluten-free foods are attractive to consumers (Taylor et al. 2006). Second, consumer demand for GMO food products has enhanced... sorghum is becoming more popular for several reasons. Sorghum is gluten free, making it desirable in the gluten-free food market (Taylor 2006). Second, consumer demand for GMO food products has enhanced food sorghum because there are no GMO sorghums...

  12. Quantitative trait loci analysis to identify modifiers genes of the gene opaque2 in maize endosperm 

    E-Print Network [OSTI]

    Gutierrez Rojas, Libardo Andres

    2009-05-15

    The protein quality of maize can be improved by replacing normal Opaque2 alleles with non-functional recessive alleles opaque2 (o2). The allele o2 produces a severe phenotype with soft endosperm enhancing its protein quality ...

  13. IDENTIFICATION OF DROUGHT-RELATED QUANTITATIVE TRAIT LOCI (QTLs) IN SUGARCANE (Saccharum spp.) USING GENIC MARKERS 

    E-Print Network [OSTI]

    Sharma, Vivek

    2011-08-08

    associations in this study. Fifty-six polymorphisms produced by 13 EST-SSR primers were used to produce genetic similarity matrix for 80 genotypes. Dendrogram prepared from this genetic similarity matrix will be useful in selecting parents carrying diversity...

  14. Expression quantitative trait loci detected in cell lines are often present in primary tissues

    E-Print Network [OSTI]

    Coop, Graham

    conducted in lympho- blastoid cell lines (LCLs), rather than primary tissues, mostly by using the Hap assays in LCLs may often be the only feasible approach (e.g. 16) to further study the potential function the overlap of eQTLs found in LCLs and primary tissues. For one, differences in study designs, platforms

  15. Quantitative trait loci(qtl) analysis of yield components and heat tolerance in wheat (Triticum aestivum) 

    E-Print Network [OSTI]

    Do, Jung Hwa

    2009-05-15

    This study was conducted to identify and map QTLs for yield components and heat tolerance of wheat in response to two kinds of heat treatment (short term-and long term-heat treatment) during seed formation in a set of 62 ...

  16. Quantitative trait loci affecting the agronomic performance of a Sorghum bicolor (L.) Moench recombinant inbred restorer line population 

    E-Print Network [OSTI]

    Moran Maradiaga, Jorge Luis

    2004-09-30

    environments........................................................................................... 109 5 Putative tillering QTL at individual and across environments................ 114 6 Putative panicle length QTL at individual and across...). Compared to a few years ago, germplasm selection is now being conducted in harsher environments; lower moisture availability, increased disease and insect pressure, and higher temperatures are among the environmental factors that have a direct impact...

  17. Copyright 1998 by the Genetics Society of America Quantitative Trait Loci for Honey Bee Stinging Behavior and Body Size

    E-Print Network [OSTI]

    Pittendrigh, Barry

    a queen of European origin and a drone descended from an African subspecies. Haploid drones from scored from the haploid drone fathers of these colonies. Wings of workers and drones were used

  18. Behavior Genetics, Vol. 33, No. 3, May 2003 ( 2003) Quantitative Trait Loci that Influence the Expression of

    E-Print Network [OSTI]

    Pittendrigh, Barry

    of workers, which are non-reproductive females and hundreds of drones that are males. Female honey bees conditions, queens mate with 10 to 17 drones (Adams et al., 1977) and as a consequence, colonies are composed of sub- families of workers that share the same queen mother but different drone fathers. All workers

  19. Genetic risk factors for stroke-related quantitative traits and their associated ischaemic stroke subtypes 

    E-Print Network [OSTI]

    Paternoster, Lavinia

    2009-01-01

    ) between E4 and E3, and E3 and E2). No polymorphism has shown a convincing association with WMHs and interestingly APOE appears unlikely to be associated with this trait. This is consistent with previous work that shows that APOE is associated with large...

  20. Characterization of Quantitative Loci for Morphological and Anatomical Root Traits on the Short Arm of Chromosome 1 of Rye in Bread Wheat

    E-Print Network [OSTI]

    Sharma, Sundrish

    2009-01-01

    bread wheat 'Pavon'. Crop Science 43:710-717 Esau K (1965)bread wheat 'Pavon'. Crop Science 43:710-717 Endo TR (1988)42- chromosome triticale. Crop Science 16:688-693 Hackauf B,

  1. Two-trait-locus linkage analysis: A powerful strategy for mapping complex genetic traits

    SciTech Connect (OSTI)

    Schork, N.J.; Boehnke, M. (Univ. of Michigan, Ann Arbor, MI (United States)); Terwilliger, J.D.; Ott, J. (Columbia Univ., New York, NY (United States))

    1993-11-01

    Nearly all diseases mapped to date follow clear Mendelian, single-locus segregation patterns. In contrast, many common familial diseases such as diabetes, psoriasis, several forms of cancer, and schizophrenia are familial and appear to have a genetic component but do not exhibit simple Mendelian transmission. More complex models are required to explain the genetics of these important diseases. In this paper, the authors explore two-trait-locus, two-marker-locus linkage analysis in which two trait loci are mapped simultaneously to separate genetic markers. The authors compare the utility of this approach to standard one-trait-locus, one-marker-locus linkage analysis with and without allowance for heterogeneity. The authors also compare the utility of the two-trait-locus, two-marker-locus analysis to two-trait-locus, one-marker-locus linkage analysis. For common diseases, pedigrees are often bilineal, with disease genes entering via two or more unrelated pedigree members. Since such pedigrees often are avoided in linkage studies, the authors also investigate the relative information content of unilineal and bilineal pedigrees. For the dominant-or-recessive and threshold models that the authors consider, the authors find that two-trait-locus, two-marker-locus linkage analysis can provide substantially more linkage information, as measured by expected maximum lod score, than standard one-trait-locus, one-marker-locus methods, even allowing for heterogeneity, while, for a dominant-or-dominant generating model, one-locus models that allow for heterogeneity extract essentially as much information as the two-trait-locus methods. For these three models, the authors also find that bilineal pedigrees provide sufficient linkage information to warrant their inclusion in such studies. The authors discuss strategies for assessing the significance of the two linkages assumed in two-trait-locus, two-marker-locus models. 37 refs., 1 fig., 4 tabs.

  2. Derived critical loci I -Basics Gabriele Vezzosi

    E-Print Network [OSTI]

    Vezzosi, Gabriele

    Derived critical loci I - Basics Gabriele Vezzosi Dipartimento di Sistemi ed Informatica Universit`a di Firenze Italy Notes ­ September 2011 Contents 1 Introduction 1 2 Koszul complexes and derived zero . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2.2 Affine derived zero loci . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2

  3. Quantitative Genetics, House Sparrows and a Multivariate

    E-Print Network [OSTI]

    Steinsland, Ingelin

    NTNU Quantitative Genetics, House Sparrows and a Multivariate Gaussian Markov Random Field Model Quantitative Genetics, House Sparrows and a Multivariate Gaussian Markov Random Field Model ­ p.1/25 #12;NTNU used in animal and plant breeding. In this study: Wild life population Several traits simultaneously

  4. Design and analysis of genetical genomics studies and their potential applications in livestock research 

    E-Print Network [OSTI]

    Lam, Alex C.

    2009-01-01

    Quantitative Trait Loci (QTL) mapping has been widely used to identify genetic loci attributable to the variation observed in complex traits. In recent years, gene expression phenotypes have emerged as a new type of ...

  5. RESEARCH PAPER Identification of loci affecting flavour volatile emissions in

    E-Print Network [OSTI]

    Klee, Harry J.

    RESEARCH PAPER Identification of loci affecting flavour volatile emissions in tomato fruits Denise shown that emissions of carotenoid-derived volatiles were directly correlated with the fruit carotenoid

  6. Mapping autism risk loci using genetic linkage and chromosomal rearrangements

    E-Print Network [OSTI]

    Mapping autism risk loci using genetic linkage and chromosomal rearrangements The Autism Genome Project Consortium1 Autism spectrum disorders (ASDs) are common, heritable neurodevelopmental conditions as promising candidates for contributing to ASDs. Autism is a neurodevelopmental disorder characterized

  7. Research article Measuring natural selection on proportional traits

    E-Print Network [OSTI]

    Stinchcombe, John

    Research article Measuring natural selection on proportional traits: comparisons of three types differentials and gradients for a unique and biologically important class of traits: proportional traits between unstandardized and variance-standardized selection gradients for proportional traits

  8. Microfluidics for quantitative biology

    E-Print Network [OSTI]

    Adler, Micha

    2012-01-01

    CALIFORNIA, SAN DIEGO Microfluidics for Quantitative BiologyChapter 1: Microfluidics.………………………………………………………..… 1 1.1OF THE DISSERTATION Microfluidics for Quantitative Biology

  9. Genetic dissection of bioenerrgy traits in sorghum

    SciTech Connect (OSTI)

    Vermerris, Wilfred; Kresovich, Stephen; Murray, Seth; Pedersen, Jeffery; Rooney, William; Sattler, Scott.

    2012-06-15

    Specific Objectives: 1. To identify the gene(s) underlying a major QTL for stem sugar concentration located on chromosome 3. 2. To identify QTL for stem juice volume and stalk sugar concentration and to identify the underlying genes. 3. To classify 60 novel sorghum bmr mutants from the USDA TILLING population in allelic groups based on cell wall chemistry and allelism tests. 4. To select representative bmr mutants from each allelic group and selected NIR spectral mutants for their potential value as feedstock for ethanol production. 5. To clone and characterize those Bmr genes that represent loci other than Bmr12 and Bmr6 using a mapping and a candidate gene approach. Objective 1 The experiments for this objective are largely complete and the data have been analyzed. Data interpretation and follow-up experiments are still in progress. A manuscript is in preparation (Vermerris et al.; see publication list for full details). The main results are: 1) 16 cDNA libraries were prepared and sequenced at Cornell University. The libraries represent internode tissue and flag leaf tissue at booting, internode tissue and peduncle at soft-dough stage, from two plants per sampling time with the Rio allele for the QTL on chromosome 3, and two plants with the BTx623 allele on chromosome 3 (4 tissues x 2 genotypes x 2 replicates) 2) 480 million 86-nucleotide reads were generated from four lanes of Illuminia HiSeqII 3) 74% of the reads could be mapped to the sorghum transcriptome, indicative of good sequence quality 4) Of the 216 genes within the QTL, 17 genes were differentially expressed among plants with and without the Rio QTL. None of these 17 genes had obvious roles in sucrose metabolism 5) Clustering algorithms identified a group of 721 co-expressed genes. One of these genes is a sucrose synthase gene. This cluster also contains 10 genes from the QTL. 6) Among these co-expressed genes are regulatory genes for which knock-out lines in Arabidopsis have been obtained. Analysis of these lines is in progress. Objective 2 The experiments from this objective have been completed and the data were published in the journal Crop Science by Felderhoff et al. (2012). A second publication by Felderhoff et al. is in progress (see publication list for full details). The experiments were based on a mapping population derived from the sweet sorghum 'Rio' and the dry-stalk grain sorghum BTx3197. The main findings were: 1) The apparent juiciness of the sorghum stalk, based on the appearance of a cut stem surface (moist vs. pithy), is not representative of the moisture content of the stalk. This was surprising, as pithy stalks have been associated with low moisture content. This means that in order to assess 'juiciness', a different evaluation needs to be used, for example by removing juice with a roller press or by measuring the difference in mass between a fresh and dried stalk segment. 2) A total of five QTLs associated with juice volume (corrected for height) or moisture content were identified, but not all QTLs were detected in all environments, providing evidence for genotype x environment interactions. This finding complicates breeding for juice volume using marker-assisted selection. 3) The QTL for sugar concentration identified on chromosome 3, and the subject of Objective 1, was confirmed in this mapping population, but unlike in previous studies (Murray et al., 2008), the presence of this QTL was associated with negative impacts on agronomic performance (fresh and dry biomass yield, juice yield). Consequently, introgression of the Brix QTL from Rio as part of a commercial breeding program will require monitoring of the precise impacts of this QTL on agronomic performance. 4) The absence of dominance effects for the Brix trait (= sugar concentration) indicated that Brix must be high in both parents to produce high Brix in hybrids. This means an extra constraint on the development of hybrid parents. With the results from Objective 1, the selection of progeny containing favorable alleles for sugar concentration is expected to be more efficient.

  10. Genomic architecture of risk loci associated with autoimmunity (7th Annual SFAF Meeting, 2012)

    ScienceCinema (OSTI)

    Wakeland, Ward [University of Texas Southwestern Medical Center

    2013-02-11

    Ward Wakeland on "Genomic architecture of risk loci associated with autoimmunity" at the 2012 Sequencing, Finishing, Analysis in the Future Meeting held June 5-7, 2012 in Santa Fe, New Mexico.

  11. Fetal weight at term influenced by H-2-associated loci

    SciTech Connect (OSTI)

    Tyan, M.L.

    1994-01-01

    Pregnant mice which in theory differ only in the region of the major histocompatibility complex (MHC) on chromosome 17 (C57BL/10, the inbred partner [host strain], and B10.D2, B10.BR, BI0.A, B10.A[2R], Bl0.A[5R], B10.A[15R] and B10.A[18R]) were sacrificed on the 11th and 18th days of gestation, and the fetuses were sexed and weighed. Fetuses from reciprocal crosses between B10.A and B10BR, B10.D2 and C57BL/10 were weighed and sexed on the 18th day of gestation. It was found that (i) fetal weights were not significantly different among the strains examined on day 11 (Bl0.BR, B10.A[15R] and B10.A[18R]), (ii) B10.BR fetuses of both sexes weighed significantly less than fetuses from the other strains on day 18, (iii) B10.D2 18-day-old male but not female fetuses were heavier than the males from the other strains (this difference was not present when corrections for litter size were made), (iv) the fetuses from the B10.A x B10.BR cross were the smallest, those from the B10.D2 x 810.A cross the largest, and those from the B10.A x C57BL/10 crosses intermediate, and (v) maternal effects were noted In the B10.A x B10.BR and B10.A x B10.D2 but not the B10.A x C57BL/10 crosses. The results suggest that there are two or more MHC associated loci that influence growth rate in late gestation. Among the candidate genes are Ped and Igfr II. 17 refs., 4 tabs.

  12. The temporal relationship of personality traits to personality disorders 

    E-Print Network [OSTI]

    Warner, Megan Beth

    2002-01-01

    Personality disorders are presumed to be stable because of underlying stable and maladaptive personality traits. It has been argued that it is the stability of these traits that makes personality disorders difficult to treat. Previous research has...

  13. Does selection for production traits affect the ability to cope with pathogens? 

    E-Print Network [OSTI]

    Coltherd, Jennifer Carolyn

    2011-06-27

    Phenotypic selection for production traits causes changes in the underlying genetics of the animal. As such, intensive selection on one trait may have consequences on other traits. Indeed alterations to traits seemingly ...

  14. Life history of a malaria parasite (Plasmodium mexicanum): independent traits

    E-Print Network [OSTI]

    Schall, Joseph J.

    Life history of a malaria parasite (Plasmodium mexicanum): independent traits and basis infections in the life-history traits which de˘ne its blood-dwelling stages. Such variation in life historiesˇects producing the variation.We studied 11 life-history traits in 120 induced infections of P. mexicanum in its

  15. Try-A Global Database of Plant Traits

    SciTech Connect (OSTI)

    Thornton, Peter E [ORNL

    2011-01-01

    Plant traits the morphological, anatomical, physiological, biochemical and phenological characteristics of plants and their organs determine how primary producers respond to environmental factors, affect other trophic levels, influence ecosystem processes and services and provide a link from species richness to ecosystem functional diversity. Trait data thus represent the raw material for a wide range of research from evolutionary biology, community and functional ecology to biogeography. Here we present the global database initiative named TRY, which has united a wide range of the plant trait research community worldwide and gained an unprecedented buy-in of trait data: so far 93 trait databases have been contributed. The data repository currently contains almost three million trait entries for 69 000 out of the world s 300 000 plant species, with a focus on 52 groups of traits characterizing the vegetative and regeneration stages of the plant life cycle, including growth, dispersal, establishment and persistence. A first data analysis shows that most plant traits are approximately log-normally distributed, with widely differing ranges of variation across traits. Most trait variation is between species (interspecific), but significant intraspecific variation is also documented, up to 40% of the overall variation. Plant functional types (PFTs), as commonly used in vegetation models, capture a substantial fraction of the observed variation but for several traits most variation occurs within PFTs, up to 75% of the overall variation. In the context of vegetation models these traits would better be represented by state variables rather than fixed parameter values. The improved availability of plant trait data in the unified global database is expected to support a paradigm shift from species to trait-based ecology, offer new opportunities for synthetic plant trait research and enable a more realistic and empirically grounded representation of terrestrial vegetation in Earth system models.

  16. Using Extended Genealogy to Estimate Components of Heritability for 23 Quantitative and Dichotomous Traits

    E-Print Network [OSTI]

    Zaitlen, Noah

    Important knowledge about the determinants of complex human phenotypes can be obtained from the estimation of heritability, the fraction of phenotypic variation in a population that is determined by genetic factors. Here, ...

  17. Salt stress response in rice: genetics, molecular biology, and comparative genomics

    E-Print Network [OSTI]

    Blumwald, Eduardo

    REVIEW Salt stress response in rice: genetics, molecular biology, and comparative genomics Chandan. Evidence show that salt tolerance in plants is a quantitative trait. Several traditional cultivars materials for donation of requisite salt tolerance genes. A large number of quantitative trait loci (QTL

  18. Primary enzyme quantitation

    DOE Patents [OSTI]

    Saunders, G.C.

    1982-03-04

    The disclosure relates to the quantitation of a primary enzyme concentration by utilizing a substrate for the primary enzyme labeled with a second enzyme which is an indicator enzyme. Enzyme catalysis of the substrate occurs and results in release of the indicator enzyme in an amount directly proportional to the amount of primary enzyme present. By quantifying the free indicator enzyme one determines the amount of primary enzyme present.

  19. Evaluation of 13 short tandem repeated loci for use in personal identification applications

    SciTech Connect (OSTI)

    Hammond, H.A.; Caskey, C.T. (Baylor College of Medicine, Houston, TX (United States)); Jin, L.; Zhong, Y.; Chakraborty, R. (Univ. of Texas Graduate School of Biomedical Sciences, Houston, TX (United States))

    1994-07-01

    Personal identification by using DNA typing methodologies has been an issue in the popular and scientific press for several years. The authors present a PCR-based DNA-typing method using 13 unlinked short tandem repeat (STR) loci. Validation of the loci and methodology has been performed to meet standards set by the forensic community and the accrediting organization for parentage testing. Extensive statistical analysis has addressed the issues surrounding the presentation of [open quotes]match[close quotes] statistics. The authors have found STR loci to provide a rapid, sensitive, and reliable method of DNA typing for parentage testing, forensic identification, and medical diagnostics. Valid statistical analysis is generally simpler than similar analysis of RFLP-VNTR results and provides powerful statistical evidence of the low frequency of random multilocus genotype matching. 54 refs., 4 figs., 6 tabs.

  20. A taxonomy of bacterial microcompartment loci constructed by a novel scoring method

    SciTech Connect (OSTI)

    Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A.; Tanaka, Mark M.

    2014-10-23

    Bacterial microcompartments (BMCs) are proteinaceous organelles involved in both autotrophic and heterotrophic metabolism. All BMCs share homologous shell proteins but differ in their complement of enzymes; these are typically encoded adjacent to shell protein genes in genetic loci, or operons. To enable the identification and prediction of functional (sub)types of BMCs, we developed LoClass, an algorithm that finds putative BMC loci and inventories, weights, and compares their constituent pfam domains to construct a locus similarity network and predict locus (sub)types. In addition to using LoClass to analyze sequences in the Non-redundant Protein Database, we compared predicted BMC loci found in seven candidate bacterial phyla (six from single-cell genomic studies) to the LoClass taxonomy. Together, these analyses resulted in the identification of 23 different types of BMCs encoded in 30 distinct locus (sub)types found in 23 bacterial phyla. These include the two carboxysome types and a divergent set of metabolosomes, BMCs that share a common catalytic core and process distinct substrates via specific signature enzymes. Furthermore, many Candidate BMCs were found that lack one or more core metabolosome components, including one that is predicted to represent an entirely new paradigm for BMC-associated metabolism, joining the carboxysome and metabolosome. By placing these results in a phylogenetic context, we provide a framework for understanding the horizontal transfer of these loci, a starting point for studies aimed at understanding the evolution of BMCs. This comprehensive taxonomy of BMC loci, based on their constituent protein domains, foregrounds the functional diversity of BMCs and provides a reference for interpreting the role of BMC gene clusters encoded in isolate, single cell, and metagenomic data. Many loci encode ancillary functions such as transporters or genes for cofactor assembly; this expanded vocabulary of BMC-related functions should be useful for design of genetic modules for introducing BMCs in bioengineering applications.

  1. A taxonomy of bacterial microcompartment loci constructed by a novel scoring method

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Axen, Seth D.; Erbilgin, Onur; Kerfeld, Cheryl A.; Tanaka, Mark M.

    2014-10-23

    Bacterial microcompartments (BMCs) are proteinaceous organelles involved in both autotrophic and heterotrophic metabolism. All BMCs share homologous shell proteins but differ in their complement of enzymes; these are typically encoded adjacent to shell protein genes in genetic loci, or operons. To enable the identification and prediction of functional (sub)types of BMCs, we developed LoClass, an algorithm that finds putative BMC loci and inventories, weights, and compares their constituent pfam domains to construct a locus similarity network and predict locus (sub)types. In addition to using LoClass to analyze sequences in the Non-redundant Protein Database, we compared predicted BMC loci found inmore »seven candidate bacterial phyla (six from single-cell genomic studies) to the LoClass taxonomy. Together, these analyses resulted in the identification of 23 different types of BMCs encoded in 30 distinct locus (sub)types found in 23 bacterial phyla. These include the two carboxysome types and a divergent set of metabolosomes, BMCs that share a common catalytic core and process distinct substrates via specific signature enzymes. Furthermore, many Candidate BMCs were found that lack one or more core metabolosome components, including one that is predicted to represent an entirely new paradigm for BMC-associated metabolism, joining the carboxysome and metabolosome. By placing these results in a phylogenetic context, we provide a framework for understanding the horizontal transfer of these loci, a starting point for studies aimed at understanding the evolution of BMCs. This comprehensive taxonomy of BMC loci, based on their constituent protein domains, foregrounds the functional diversity of BMCs and provides a reference for interpreting the role of BMC gene clusters encoded in isolate, single cell, and metagenomic data. Many loci encode ancillary functions such as transporters or genes for cofactor assembly; this expanded vocabulary of BMC-related functions should be useful for design of genetic modules for introducing BMCs in bioengineering applications.« less

  2. Localizing multiple X chromosome-linked retinitis pigmentosa loci using multilocus homogeneity tests

    SciTech Connect (OSTI)

    Ott, J.; Terwilliger, J.D. (New York State Psychiatric Institute, New York (USA)); Bhattacharya, S. (Univ. of Newcastle upon Tyne (England)); Chen, J.D.; Denton, J.; Donald, J. (Prince of Wales Hospital, Randwick (Australia)); Dubay, C.; Litt, M.; Weleber, R.G. (Oregon Health Sciences Univ., Portland (USA)); Farrar, G.J.; Humphries, P. (Trinity College, Dublin (Ireland)); Fishman, G.A.; Wong, F. (Univ. of Illinois, Chicago (USA)); Frey, D.; Maechler, M. (Institute of Medical Genetics, Zurich (Switzerland))

    1990-01-01

    Multilocus linkage analysis of 62 family pedigrees with X chromosome-linked retinitis pigmentosa (XLRP) was undertaken to determine the presence of possible multiple disease loci and to reliability estimate their map location. Multilocus homogeneity tests furnish convincing evidence for the presence of two XLRP loci, the likelihood ratio being 6.4 {times} 10{sup 9}:1 in a favor of two versus a single XLRP locus and gave accurate estimates for their map location. In 60-75% of the families, location of an XLRP gene was estimated at 1 centimorgan distal to OTC, and in 25-40% of the families, an XLRP locus was located halfway between DXS14 (p58-1) and DXZ1 (Xcen), with an estimated recombination fraction of 25% between the two XLRP loci. There is also good evidence for third XLRP locus, midway between DXS28 (C7) and DXS164 (pERT87), supported by a likelihood ratio of 293:1 for three versus two XLRP loci.

  3. Multiple Loci within the Major Histocompatibility Complex Confer Risk of Psoriasis

    E-Print Network [OSTI]

    Abecasis, Goncalo

    Multiple Loci within the Major Histocompatibility Complex Confer Risk of Psoriasis Bing-Jian Feng1 Psoriasis is a common inflammatory skin disease characterized by thickened scaly red plaques. Previously we have performed a genome-wide association study (GWAS) on psoriasis with 1,359 cases and 1,400 controls

  4. Characterization of seven new polymorphic microsatellite loci in the brilliant-thighed poison frog Allobates femoralis

    E-Print Network [OSTI]

    Ringler, Eva

    175 Characterization of seven new polymorphic microsatellite loci in the brilliant-thighed poison novel polymorphic microsatellite markers for the brilliant- thighed poison frog Allobates femoralis by the British Herpetological Society The Neotropical poison frog Allobates femoralis (Dendrobatidae) has a pan

  5. Personality traits, traitedness, and disorders: towards an enhanced understanding of trait-disorder relationships 

    E-Print Network [OSTI]

    Warner, Megan Beth

    2006-10-30

    Traitedness has been described as the �the degree to which a particular trait structure is approximated in a given person� (Tellegen, p. 28, 1991) and has been hypothesized as one explanation for findings of weak ...

  6. Vegetative morphology and trait correlations in 54 species of Commelinaceae

    E-Print Network [OSTI]

    Miller, Thomas E.

    was examined with simultaneous attention to constraints, adaptive hypotheses and relatedness. Eleven­50% of the variance in a single trait. The relationships between some traits are consistent with adaptive explanations as self-shading decreases, and that light availability governs leaf size and branching patterns

  7. Of intent and action : implementing personality traits for storytelling through concept patterns

    E-Print Network [OSTI]

    Song, Susan S. (Susan Shuchen)

    2012-01-01

    Personality traits such as "kind," "aggressive," and "brave" are integral to storytelling because they impart succinct descriptors of character personalities. Authors apply traits to characters, readers infer characters' ...

  8. The high energy semiclassical asymptotics of loci of roots of fundamental solutions for polynomial potentials

    E-Print Network [OSTI]

    Stefan Giller

    2008-05-24

    In the case of polynomial potentials all solutions to 1-D Schroedinger equation are entire functions totally determined by loci of their roots and their behaviour at infinity. In this paper a description of the first of the two properties is given for fundamental solutions for the high complex energy limit when the energy is quantized or not. In particular due to the fact that the limit considered is semiclassical it is shown that loci of roots of fundamental solutions are collected of selected Stokes lines (called exceptional) specific for the solution considered and are distributed along these lines in a specific way. A stable asymptotic limit of loci of zeros of fundamental solutions on their exceptional Stokes lines has island forms and there are infintely many of such roots islands on exceptional Stokes lines escaping to infinity and a finite number of them on exceptional Stokes lines which connect pairs of turning points. The results obtained for asymptotic roots distributions of fundamental solutions in the semiclassical high (complex) energy limit are of a general nature for polynomial potentials.

  9. Quantitative analysis of masculinity perceptions 

    E-Print Network [OSTI]

    Cima, Brian Norman

    2002-01-01

    This study was conducted using a modified version of the Brannon's Masculinity index, designed to measure quantitatively the ideologies of American masculinity. Included is an extensive literature review and an explanation ...

  10. What constrains directional selection on complex traits in the wild?

    E-Print Network [OSTI]

    Mojica, Julius Penalba

    2011-08-31

    The fact that abundant genetic variation persists within populations despite strong directional selection on complex traits is one of the unresolved conundrums in evolutionary biology. In this dissertation, I employed a ...

  11. Trait-based approaches to marine microbial ecology

    E-Print Network [OSTI]

    Barton, Andrew David

    2011-01-01

    The goal of this thesis is to understand how the functional traits of species, biotic interactions, and the environment jointly regulate the community ecology of phytoplankton. In Chapter 2, I examined Continuous Plankton ...

  12. Personality traits distinguishing dementia with Lewy bodies from Alzheimer disease

    E-Print Network [OSTI]

    Galvin, James E.; Malcom, Heather; Johnson, David Kevin; Morris, John C.

    2007-05-29

    Personality traits distinguishing dementia with Lewy bodies from Alzheimer disease James E. Galvin, MD, MPH Heather Malcom David Johnson, PhD John C. Morris, MD ABSTRACT Objective: To identify personality traits that distinguish dementia with Lewy... and differentiation in conjunction with other DLB features. METHODS Research participants. Beginning in 1979, over 3,000 individuals have been enrolled in our longitudinal studies of healthy aging and dementia and over 800 of these participants have been studied...

  13. On Incremental Quantitative Verification for Probabilistic Systems

    E-Print Network [OSTI]

    Oxford, University of

    . Quantitative verification is an automated method to establish quantitative properties of a system model, non- probabilistic verification, which, in addition to exhaustive exploration of a system modelOn Incremental Quantitative Verification for Probabilistic Systems Marta Kwiatkowska, David Parker

  14. Microfluidics for optics and quantitative cell biology

    E-Print Network [OSTI]

    Campbell, James Kyle

    2008-01-01

    OF CALIFORNIA, SAN DIEGO Microfluidics for Optics and6 2. Microfluidics for Quantitative CellTHE DISSERTATION Microfluidics for Optics and Quantitative

  15. The Identification of Two Maturity Loci Sheds Light on Photoperiodic Flowering in Sorghum 

    E-Print Network [OSTI]

    Murphy, Rebecca

    2012-10-19

    MERISTEM VIN3 COLD FLC FLC FT FD GI LIM1 AGL24 SOC1 WUS CLV TFL1 LFY CAL AP1 FLK FVE FLD LD G AAAAA FCA FPA FY SEP SEP AG AGC SEP SEPAP1 AP1 SEP AG AP3 PI SEPAP1 AP3PI A B FLC mRNA SUS1 SUC2 SUC6 SUC7CH2OH CH2OH CH2... THE IDENTIFICATION OF TWO MATURITY LOCI SHEDS LIGHT ON PHOTOPERIODIC FLOWERING IN SORGHUM A Dissertation by REBECCA LEA MURPHY Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements...

  16. Nondetectability of restriction fragments and independence of DNA fragment sizes within and between loci in RFLP typing of DNA

    SciTech Connect (OSTI)

    Chakraborty, R.; Zhong, Y.; Jin, L. ); Budowle, B. )

    1994-08-01

    The authors provide experimental evidence showing that, during the restriction-enzyme digestion of DNA samples, some of the HaeIII-digested DNA fragments are small enough to prevent their reliable sizing on a Southern gel. As a result of such nondetectability of DNA fragments, individuals who show a single-band DNA profile at a VNTR locus may not necessarily be true homozygotes. In a population database, when the presence of such nondetectable alleles is ignored, they show that a pseudodependence of alleles within as well as across loci may occur. Using a known statistical method, under the hypothesis of independence of alleles within loci, they derive an efficient estimate of null allele frequency, which may be subsequently used for testing allelic independence within and across loci. The estimates of null allele frequencies, thus derived, are shown to agree with direct experimental data on the frequencies of HaeIII-null alleles. Incorporation of null alleles into the analysis of the forensic VNTR database suggests that the assumptions of allelic independence within and between loci are appropriate. In contrast, a failure to incorporate the occurrence of null alleles would provide a wrong inference regarding the independence of alleles within and between loci. 47 refs., 2 figs., 4 tabs.

  17. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment

    SciTech Connect (OSTI)

    Zhang, Pengju; Lo, Alvin; Huang, Yurong; Huang, Ge; Liang, Guozhou; Mott, Joni; Karpen, Gary H.; Blakely, Eleanor A.; Bissell, Mina J.; Barcellos-Hoff, Mary Helen; Snijders, Antoine M.; Mao, Jian-Hua

    2015-03-09

    The interplay between host genetics, tumor microenvironment and environmental exposure in cancer susceptibility remains poorly understood. Here we assessed the genetic control of stromal mediation of mammary tumor susceptibility to low dose ionizing radiation (LDIR) using backcrossed F1 into BALB/c (F1Bx) between cancer susceptible (BALB/c) and resistant (SPRET/EiJ) mouse strains. Tumor formation was evaluated after transplantation of non-irradiated Trp53-/- BALB/c mammary gland fragments into cleared fat pads of F1Bx hosts. Genome-wide linkage analysis revealed 2 genetic loci that constitute the baseline susceptibility via host microenvironment. However, once challenged with LDIR, we discovered 13 additional loci that were enriched for genes involved in cytokines, including TGF?1 signaling. Surprisingly, LDIR-treated F1Bx cohort significantly reduced incidence of mammary tumors from Trp53-/- fragments as well as prolonged tumor latency, compared to sham-treated controls. We demonstrated further that plasma levels of specific cytokines were significantly correlated with tumor latency. Using an ex vivo 3-D assay, we confirmed TGF?1 as a strong candidate for reduced mammary invasion in SPRET/EiJ, which could explain resistance of this strain to mammary cancer risk following LDIR. Our results open possible new avenues to understand mechanisms of genes operating via the stroma that affect cancer risk from external environmental exposures.

  18. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhang, Pengju; Lo, Alvin; Huang, Yurong; Huang, Ge; Liang, Guozhou; Mott, Joni; Karpen, Gary H.; Blakely, Eleanor A.; Bissell, Mina J.; Barcellos-Hoff, Mary Helen; et al

    2015-03-09

    The interplay between host genetics, tumor microenvironment and environmental exposure in cancer susceptibility remains poorly understood. Here we assessed the genetic control of stromal mediation of mammary tumor susceptibility to low dose ionizing radiation (LDIR) using backcrossed F1 into BALB/c (F1Bx) between cancer susceptible (BALB/c) and resistant (SPRET/EiJ) mouse strains. Tumor formation was evaluated after transplantation of non-irradiated Trp53-/- BALB/c mammary gland fragments into cleared fat pads of F1Bx hosts. Genome-wide linkage analysis revealed 2 genetic loci that constitute the baseline susceptibility via host microenvironment. However, once challenged with LDIR, we discovered 13 additional loci that were enriched for genesmore »involved in cytokines, including TGF?1 signaling. Surprisingly, LDIR-treated F1Bx cohort significantly reduced incidence of mammary tumors from Trp53-/- fragments as well as prolonged tumor latency, compared to sham-treated controls. We demonstrated further that plasma levels of specific cytokines were significantly correlated with tumor latency. Using an ex vivo 3-D assay, we confirmed TGF?1 as a strong candidate for reduced mammary invasion in SPRET/EiJ, which could explain resistance of this strain to mammary cancer risk following LDIR. Our results open possible new avenues to understand mechanisms of genes operating via the stroma that affect cancer risk from external environmental exposures.« less

  19. Quantitative Risk Management Rudiger Frey

    E-Print Network [OSTI]

    Frey, Rüdiger

    Lecture Quantitative Risk Management R¨udiger Frey Universit¨at Leipzig Wintersemester 2010 risk management C. Introduction to Portfolio Credit Derivatives c 2010 (Frey) 1 #12;A. Introduction of counterparties. Measuring and management of credit risk is of high importance for financial institutions

  20. Automated Quantitative Histomorphometry in Osteoarthritis

    E-Print Network [OSTI]

    Integrator System was designed and developed using standard methods of Object Oriented Analysis and DesignAutomated Quantitative Histomorphometry in Osteoarthritis Eigil Mřlvig Jensen Supervised by Bjarne;Technical University of Denmark Informatics and Mathematical Modelling Building 321, DK-2800 Kongens Lyngby

  1. Ethnic differentiation at VNTR loci, with special reference to forensic applications

    SciTech Connect (OSTI)

    Devlin, B.; Risch, N. )

    1992-09-01

    Allele-rich VNTR loci provide valuable information for forensic inference. Interpretation of this information is complicated by measurement error, which renders discrete alleles difficult to distinguish. Two methods have been used to circumvent this difficulty-i.e, binning methods and direct evaluation of allele frequencies, the latter achieved by modeling the data as a mixture distribution. The authors use this modeling approach to estimate the allele frequency distributions for two loci-D17S79 and D2S44-for black, Caucasian, and Hispanic samples from the Lifecodes and FBI data bases. The databases are differentiated by the restriction enzyme used: PstI (Lifecodes) and HaeIII (FBI). The results show that alleles common in one ethnic group are almost always common in all ethnic groups, and likewise for rare alleles; this pattern holds for both loci. Gene diversity, or heterozygosity, measured as one minus the sum of the squared allele frequencies, is greater for D2S44 than for D17S79, in both data bases. The average gene diversity across ethnic groups when PstI (HaeIII) is used is .918 (.918) for D17S79 and is .985 (.983) for D2S44. The variance in gene diversity among ethnic groups is greater for D17S79 than for D2S44. The number of alleles, like the gene diversity, is greater for D2S44 than for D17S79. The mean numbers of alleles across ethnic groups, estimated from the PstI (HaeIII) data, are 40.25 (41.5) for D 17S79 and 104 (103) for D2S44. The number of alleles is correlated with sample size. The authors use the estimated allele frequency distributions for each ethnic group to explore the effects of unwittingly mixing populations and thereby violating independence assumptions. They show that, even in extreme cases of mixture, the estimated genotype probabilities are good estimates of the true probabilities, contradicting recent claims. 35 refs., 9 figs., 3 tabs.

  2. Multiplex DNA Typing of Short-Tandem-Repeat Loci on the Y Chromosome

    E-Print Network [OSTI]

    Redd, Alan J.; Clifford, Stephanie L.; Stoneking, Mark

    1997-08-01

    buffer [10 mM Tris-HCI, pH 8.3; 5rrwi NH4CI; 50mw KCI; 1.5MM MgCI2 (Blanchard et a!., 1993)], 1.0 mg/ml of Bovine Serum Albumin, 0.25 units of AmpliTaq® DNA polymerase (Perkin Elmer), 200 jxm of each dNTP; and 0.32 jxM DYS394 Forward (F) and Reverse... (Nei 1987)] for the four loci are: 0.67, 0.78, 0.53, and 0.48 for DYS19, DYS390, DYS391, and DYS393 respectively. We also measure var­ iance in repeat size (Jorde etal., 1997), a genetic diversity measure that can be related to the single...

  3. Neural network detected in a presumed vestigial trait: ultrastructure of the

    E-Print Network [OSTI]

    Reimchen, Thomas E.

    Neural network detected in a presumed vestigial trait: ultrastructure of the salmonid adipose fin J; nerve network; astrocytes; primary cilia; fisheries 1. INTRODUCTION The long-term persistence of traits

  4. Genomic Diversity at Thirteen Short Tandem Repeat Loci in a Substructured Caste Population, Golla, of Southern Andhra Pradesh, India

    E-Print Network [OSTI]

    Reddy, B. Mohan; Sun, Guangyun; Luis, Javier Rodriguez; Crawford, Michael H.; Hemam, Natabar Shyam; Deka, Ranjan

    2001-04-01

    homogeneous, and strictly endogamous. Blood samples were drawn from 317 individuals from 30 Golla villages. The 13 STR loci analyzed in five standard multiplex polymerase chain reactions were: (1) CSF1R, TH01, and PLA2A; (2) F13A1, CYP19, and LPL; (3) D21S1446...

  5. Variation in ecogeographical traits of pecan cultivars and provenances 

    E-Print Network [OSTI]

    Sagaram, Madhulika

    2009-05-15

    and provenances (i.e., the area of origin of seed). An assessment of leaf anatomical traits of pecan cultivars (Pawnee, Mohawk and Starking Hardy Giant) collected from three locations (Tifton, GA., Chetopa, KS., and Stillwater, OK.) was conducted to provide...

  6. LETTERS TO NATURE Effects of gamete traits on

    E-Print Network [OSTI]

    McQuade, D. Tyler

    #12;LETTERS TO NATURE Effects of gamete traits on fertilization in the sea and the evolution, females by resources3. Evidence of sperm competition comes from internal fertilizers, or cases where sperm dimorphism, in which sperm limitation is important, and natural selection for enhanced fertilization acts

  7. Automatic Point-based Facial Trait Judgments Evaluation Mario Rojas Q.1

    E-Print Network [OSTI]

    Todorov, Alex

    Automatic Point-based Facial Trait Judgments Evaluation Mario Rojas Q.1 , David Masip1 dimensions to automat- ically learn a trait predictor from facial pixel images. We study whether trait evaluations performed by humans can be learned using machine learning classifiers, and used later in automatic

  8. Geographical Variation in Selection, from Phenotypes to Molecules

    E-Print Network [OSTI]

    Kelly, John K.

    2006-04-01

    and quantitative trait variation within species. Keywords: balancing selection, neutrality tests, quantitative trait loci, Tajima’s D, ZnS. Natural populations exhibit a great deal of genetic vari- ation at both molecular and phenotypic levels. The amount... the number of polymorphic sites (S) and average pairwise difference (p) among sequences in the sample. I also calculate the neutrality test statistics D (Tajima 1989) and ZnS (Kelly 1997) and determine whether these values are statistically significant...

  9. QUANTITATIVE RISK MANAGEMENT: CONCEPTS, TECHNIQUES AND TOOLS*

    E-Print Network [OSTI]

    Embrechts, Paul

    QUANTITATIVE RISK MANAGEMENT: CONCEPTS, TECHNIQUES AND TOOLS* Paul Embrechts Department of Mathematics ETH Zurich www.math.ethz.ch/~embrechts #12;QUANTITATIVE RISK MANAGEMENT: CONCEPTS, TECHNIQUES Theorems of Quantitative Risk Management · PE's Desert-Island Copula · Example 1: Credit Risk · Example 2

  10. Quantitative Characterization of Nanostructured Materials

    SciTech Connect (OSTI)

    Dr. Frank Bridges, University of California-Santa Cruz

    2010-08-05

    The two-and-a-half day symposium on the "Quantitative Characterization of Nanostructured Materials" will be the first comprehensive meeting on this topic held under the auspices of a major U.S. professional society. Spring MRS Meetings provide a natural venue for this symposium as they attract a broad audience of researchers that represents a cross-section of the state-of-the-art regarding synthesis, structure-property relations, and applications of nanostructured materials. Close interactions among the experts in local structure measurements and materials researchers will help both to identify measurement needs pertinent to â??real-worldâ?ť materials problems and to familiarize the materials research community with the state-of-the-art local structure measurement techniques. We have chosen invited speakers that reflect the multidisciplinary and international nature of this topic and the need to continually nurture productive interfaces among university, government and industrial laboratories. The intent of the symposium is to provide an interdisciplinary forum for discussion and exchange of ideas on the recent progress in quantitative characterization of structural order in nanomaterials using different experimental techniques and theory. The symposium is expected to facilitate discussions on optimal approaches for determining atomic structure at the nanoscale using combined inputs from multiple measurement techniques.

  11. Quantitative patterns in drone wars

    E-Print Network [OSTI]

    Garcia-Bernado, J; Johnson, N F

    2014-01-01

    Attacks by drones (i.e., unmanned combat air vehicles) continue to generate heated political and ethical debates. Here we examine instead the quantitative nature of drone attacks, focusing on how their intensity and frequency compares to other forms of human conflict. In contrast to the power-law distribution found recently for insurgent and terrorist attacks, we find that the severity of attacks is well described by a lognormal distribution, indicating that the dynamics underlying drone attacks differ from these other forms of human conflict. Likewise, the pattern in the timing of attacks is consistent with one side having almost complete control. We show that these novel features can be reproduced and understood using a generative mathematical model in which resource allocation to the dominant side is regulated through a feedback loop.

  12. Risk Analysis of Prostate Cancer in PRACTICAL, a Multinational Consortium, Using 25 Known Prostate Cancer Susceptibility Loci

    E-Print Network [OSTI]

    Amin Al Olama, Ali; Benlloch, Sara; Antoniou, Antonis C.; Giles, Graham G.; Severi, Gianluca; Neal, David; Hamdy, Freddie C.; Donovan, Jenny L.; Muir, Kenneth; Schleutker, Johanna; Henderson, Brian E.; Haiman, Christopher; Schumacher, Fredrick R.; Pashayan, Nora; Pharoah, Paul D. P.; Ostrander, Elaine A.; Stanford, Janet L.; Batra, Jyotsna; Clements, Judith A.; Chambers, Suzanne K.; Weischer, Maren; Nordestgaard, Břrge G.; Ingles, Sue Ann; Sorensen, Karina D.; Orntoft, Torben F.; Park, Jong Y.; Cybulski, Cezary; Maier, Christiane; Doerk, Thilo; Dickinson, Joanne L.; Cannon-Albright, Lisa; Brenner, Hermann; Rebbeck, Timothy R.; Zeigler-Johnson, Charnita; Habuchi, Tomonori; Thibodeau, Stephen N.; Cooney, Kathleen; Chappuis, Pierre O.; Hutter, Pierre; Kaneva, Radka P.; Foulkes, William D.; Zeegers, Maurice P.; Lu, Yong-Jie; Zhang, Hong-Wei; Stephenson, Robert; Cox, Angela; Southey, Melissa C.; Spurdle, Amanda B.; FitzGerald, Liesel; Leongamornlert, Daniel; Saunders, Edward; Tymrakiewicz, Malgorzata; Guy, Michelle; Dadaev, Tokhir; Little, Sarah J.; Govindasami, Koveela; Sawyer, Emma; Wilkinson, Rosemary; Herkommer, Kathleen; Hopper, John L.; Lophatonanon, Aritaya; Rinckleb, Antje E.; Kote-Jarai, Zsofia; Eeles, Rosalind A.; Easton, Douglas F.

    2015-04-02

    & Prevention.pdf.txt Content-Type text/plain; charset=UTF-8 1 Risk Analysis of Prostate Cancer in PRACTICAL, a Multinational Consortium, Using 25 Known Prostate Cancer Susceptibility Loci Ali Amin Al Olama1,56, Sara Benlloch1,56, Antonis C... @medschl.cam.ac.uk Tel: 0044 1223 748638 Fax: 0044 1223 748628 Running Title: Risk Analysis of Prostate Cancer in PRACTICAL Consortium Keywords: Prostate Cancer risk, Genetic and Molecular Epidemiology, Genitourinary Cancers: Prostate, Risk...

  13. Optimization Online - Quantitative Stability Analysis of Stochastic ...

    E-Print Network [OSTI]

    Jie Zhang

    2015-06-03

    Jun 3, 2015 ... Quantitative Stability Analysis of Stochastic Quasi-Variational Inequality Problems and Applications. Jie Zhang(zhangjie04212001 ***at*** ...

  14. Geothermal Reconnaissance From Quantitative Analysis Of Thermal...

    Open Energy Info (EERE)

    Geothermal Reconnaissance From Quantitative Analysis Of Thermal Infrared Imagery Jump to: navigation, search OpenEI Reference LibraryAdd to library Reference: Geothermal...

  15. Quantitative Modeling of High Temperature Magnetization Dynamics

    SciTech Connect (OSTI)

    Zhang, Shufeng

    2009-03-01

    Final Technical Report Project title: Quantitative Modeling of High Temperature Magnetization Dynamics DOE/Office of Science Program Manager Contact: Dr. James Davenport

  16. Combining Quantitative Electrochemistry and Electron Microscopy...

    Office of Scientific and Technical Information (OSTI)

    Combining Quantitative Electrochemistry and Electron Microscopy to Study Reversible Lithiation of Silicon Nanowires. Citation Details In-Document Search Title: Combining...

  17. DAIRY FARM PROFITABILITY ENHANCED BY APPLICATION OF QUANTITATIVE GENETICS

    E-Print Network [OSTI]

    Obbard, Darren

    , udder composite, locomotion · variance traits e.g. energy balance, body condition · production traits through better breeding selection reduces the number of replacement females which need to be reared (a 2 are used by breeding companies and by farmers to improve the genetic merit of the national herd

  18. Bacterial traits, organism mass, and numerical abundance in the detrital soil food web of Dutch

    E-Print Network [OSTI]

    Cohen, Joel E.

    LETTER Bacterial traits, organism mass, and numerical abundance in the detrital soil food web.mulder@rivm.nl Abstract This paper compares responses to environmental stress of the ecophysiological traits of organisms in the detrital soil food webs of grasslands in the Netherlands, using the relationship between average body mass

  19. Understanding trait interactions and their impacts on growth in Scots pine branches across Europe

    E-Print Network [OSTI]

    Mencuccini, Maurizio

    ­effect relationships between anatomical traits, hydraulic traits and branch growth, we measured for each branch Studies, Wageningen University, PO 47, NL­6700 AA Wageningen, The Netherlands; 2 CREAF / Ecology Unit: the tracheid hydraulic diame- ter, double cell wall thickness, cell lumen span area, wood density, cavitation

  20. Heterogeneity of distribution for growth traits between Angus and Brahman backcross embryo transfer cattle 

    E-Print Network [OSTI]

    Cleere, Jason James

    1998-01-01

    was significant for all traits and SEX was significant for all traits excluding FG. Birth year and birth season were significant effects for WW, GG, FG, and SW. Classes set according to industry standards were BW (4 kg increments), WW (50 kg increments), GG (40 kg...

  1. Bayesian Inference of Multiple-Traits in a House Sparrow Population using the Animal Model

    E-Print Network [OSTI]

    Steinsland, Ingelin

    Bayesian Inference of Multiple-Traits in a House Sparrow Population using the Animal Model Ingelin- sidered in the further analysis. Hestmannřy (one of the study islands) Data ·Most house sparrows of six island are known. The Animal Model For bird i (i = 1, . . . , 1004): observed traits =fixed +genetic

  2. The importance of retaining a phylogenetic perspective in traits-based community analyses

    SciTech Connect (OSTI)

    Poteat, Monica D; Buchwalter, David; Jacobus, Luke

    2015-01-01

    1) Many environmental stressors manifest their effects via physiological processes (traits) that can differ significantly among species and species groups. We compiled available data for three traits related to the bioconcentration of the toxic metal cadmium (Cd) from 42 aquatic insect species representing orders Ephemeroptera (mayfly), Plecoptera (stonefly), and Trichoptera (caddisfly). These traits included the propensity to take up Cd from water (uptake rate constant, ku), the ability to excrete Cd (efflux rate constant, ke), and the net result of these two processes (bioconcentration factor, BCF). 2) Ranges in these Cd bioaccumulation traits varied in magnitude across lineages (some lineages had a greater tendency to bioaccumulate Cd than others). Overlap in the ranges of trait values among different lineages was common and highlights situations where species from different lineages can share a similar trait state, but represent the high end of possible physiological values for one lineage and the low end for another. 3) Variance around the mean trait state differed widely across clades, suggesting that some groups (e.g., Ephemerellidae) are inherently more variable than others (e.g., Perlidae). Thus, trait variability/lability is at least partially a function of lineage. 4) Akaike information criterion (AIC) comparisons of statistical models were more often driven by clade than by other potential biological or ecological explanation tested. Clade-driven models generally improved with increasing taxonomic resolution. 5) Together, these findings suggest that lineage provides context for the analysis of species traits, and that failure to consider lineage in community-based analysis of traits may obscure important patterns of species responses to environmental change.

  3. Early Life Measures of Size as Related to Weights and Productivity in Beef Cows and Carcass Traits in Steers 

    E-Print Network [OSTI]

    Cunningham, Samantha Fern

    2013-12-10

    and productivity of females (n = 2,800 records from 450 animals) and carcass traits (n = 450) of steers. Cattle were F1 crosses sired by Angus, Brahman, Boran, Gir, Indu-Brazil, Nellore, and Tuli. Relationships between cow traits and carcass traits of steer mates...

  4. Conserved genomic structure of the Cyp1a1 and Cyp1a2 loci and their dioxin responsive elements cluster

    E-Print Network [OSTI]

    Bradfield, Christopher A.

    Review Conserved genomic structure of the Cyp1a1 and Cyp1a2 loci and their dioxin responsivea2 DRE AHR Dioxin a b s t r a c t A thorough DNA sequence analysis reveals that the mouse Cyp1a11_1a2 junction, eight consensus dioxin responsive elements (DREs) are present and seven of the eight

  5. The role of the dioxin-responsive element cluster between the Cyp1a1 and Cyp1a2 loci in aryl

    E-Print Network [OSTI]

    Bradfield, Christopher A.

    The role of the dioxin-responsive element cluster between the Cyp1a1 and Cyp1a2 loci in aryl,3,7,8- tetrachlorodibenzo-p-dioxin (dioxin) hepatotoxicity, regulation of xenobiotic metabolism, and hepatovascular development. Each of these processes appears to be dependent on binding of the AHR to dioxin- responsive

  6. Trait Approach and Avoidance Motivation: Lateralized Neural Activity Associated with Executive Function

    E-Print Network [OSTI]

    Banich, Marie T.

    Trait Approach and Avoidance Motivation: Lateralized Neural Activity Associated with Executive of Illinois at Urbana-Champaign Abstract Motivation and executive function are both necessary neural mechanisms. However, this research has focused on state motivation, and it has not examined

  7. The Use of Traits-Based Assessment to Estimate Effects of Hydropower Projects on Fish Populations

    E-Print Network [OSTI]

    for every hydroelectric project or turbine design. We are exploring the use of a Traits-Based Assessment that influence the risk of downstream passage losses at hydroelectric power plants www.ornl.gov #12;

  8. Latin America’s New World of Work: Changing Traits of Work and Problem Solving

    E-Print Network [OSTI]

    Collier, Ruth Berins; Palmer-Rubin, Brian

    2011-01-01

    Traits of Work and Problem Solving Ruth Berins Collier Brianwork-based resources for problem solving (size of work-basedof what we will call “problem-solving activities? ” Further,

  9. RESEARCH ARTICLE Open Access Levels of autistic traits in anorexia nervosa: a

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    RESEARCH ARTICLE Open Access Levels of autistic traits in anorexia nervosa: a comparative associated with Autism Spectrum Disorders (ASD) are over-represented among patients with Anorexia Nervosa (AN and associations between these disorders. Keywords: Anorexia nervosa, Autism, Neuropsychology, Flexibility

  10. SYNTHESIS The biogeography of marine plankton traits Andrew D. Barton,1,2

    E-Print Network [OSTI]

    the export of carbon (C) from the ocean surface, the transfer of energy and organic matter to higher trophic community trait composition determines the rates of significant processes, including carbon export. Although

  11. The role of functional traits and trade-offs in structuring phytoplankton communities: scaling

    E-Print Network [OSTI]

    @kbs.msu.edu Abstract Trait-based approaches to community structure are increasingly used in terrestrial ecology. We and enzyme kinetics, define contrasting ecological strategies of nutrient acquisition. Major groups of marine

  12. On Quantitative Software Verification Marta Kwiatkowska

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    for establish- ing quantitative properties of a system model, such as the probability of battery power dropping concentrated on methods for analysing qualitative properties of system models. Many pro- grams, however to analyse system models in several application domains, including security and network protocols. However

  13. Computing Laboratory QUANTITATIVE MULTI-OBJECTIVE

    E-Print Network [OSTI]

    Norman, Gethin

    , for ex- ample, energy usage or performance metrics. Quantitative properties of these models are expressed", "energy usage" or "number of messages successfully sent". Properties of PAs can be specified using well Oxford University Computing Laboratory, Parks Road, Oxford, OX1 3QD, UK 2 School of Computing Science

  14. Quantitative multiplex detection of pathogen biomarkers

    DOE Patents [OSTI]

    Mukundan, Harshini; Xie, Hongzhi; Swanson, Basil I; Martinez, Jennifer; Grace, Wynne K

    2014-10-14

    The present invention addresses the simultaneous detection and quantitative measurement of multiple biomolecules, e.g., pathogen biomarkers through either a sandwich assay approach or a lipid insertion approach. The invention can further employ a multichannel, structure with multi-sensor elements per channel.

  15. Topics in Quantitative Risk Management Paul Embrechts

    E-Print Network [OSTI]

    Embrechts, Paul

    Topics in Quantitative Risk Management Paul Embrechts ETH-Z¨urich 1. Basel II and its risk management consequences 2. An overview of credit risk models 3. Modelling extremes: use and limitations 4@math.ethz.ch Homepage: http://www.math.ethz.ch/embrechts #12;Lecture 1: Basel II and its risk management consequences

  16. Errors in Quantitative Image Analysis due to

    E-Print Network [OSTI]

    Rubin, Daniel L.

    ; Massachusetts General Hospital, Boston, MA Abstract PURPOSE: To evaluate the ability of various software (SW different SW tools to measure compartment-specific region-of-interest intensity. RESULTS: Images generated for by the majority of tested quantitative image analysis SW tools. Incorrect image scaling leads to intensity

  17. Advances in Quantitative Verification for Ubiquitous Computing

    E-Print Network [OSTI]

    Oxford, University of

    Advances in Quantitative Verification for Ubiquitous Computing Marta Kwiatkowska Department of Computer Science, University of Oxford, Oxford, OX1 3QD Abstract. Ubiquitous computing, where computers and correcting flaws in a number of ubiquitous computing applications. In this paper, we focus on three key

  18. Quantitative organic vapor-particle sampler

    DOE Patents [OSTI]

    Gundel, Lara (Berkeley, CA); Daisey, Joan M. (Walnut Creek, CA); Stevens, Robert K. (Cary, NC)

    1998-01-01

    A quantitative organic vapor-particle sampler for sampling semi-volatile organic gases and particulate components. A semi-volatile organic reversible gas sorbent macroreticular resin agglomerates of randomly packed microspheres with the continuous porous structure of particles ranging in size between 0.05-10 .mu.m for use in an integrated diffusion vapor-particle sampler.

  19. Economics, Quantitative Emphasis BA, 2015-2016 Name ID# Date

    E-Print Network [OSTI]

    Barrash, Warren

    Economics, Quantitative Emphasis BA, 2015-2016 Name ID# Date Course Number and Title Credits Quantitative Methods in Economics 3 FF ECON 422 Econometrics 3 Upper-division economics courses 16 MATH 175

  20. The Cost of Bearing a Sword: Locomotor Costs and Compensations in Relation to a Sexually Selected Trait in Xiphophorus

    E-Print Network [OSTI]

    Oufiero, Christopher E.

    2010-01-01

    Evaluating performance costs of sexually selected traits.result in a locomotor cost. References Allen, B. J. &Levinton, J. S. (2006). Costs of bearing a sexually selected

  1. Math 576: Quantitative Risk Management lih@math.wsu.edu

    E-Print Network [OSTI]

    Li, Haijun

    Math 576: Quantitative Risk Management Haijun Li lih@math.wsu.edu Department of Mathematics Washington State University Week 3 Haijun Li Math 576: Quantitative Risk Management Week 3 1 / 26 #12;Outline Li Math 576: Quantitative Risk Management Week 3 2 / 26 #12;Purposes of Accurate Risk Assessment

  2. Math 576: Quantitative Risk Management lih@math.wsu.edu

    E-Print Network [OSTI]

    Li, Haijun

    Math 576: Quantitative Risk Management Haijun Li lih@math.wsu.edu Department of Mathematics Washington State University Week 1 Haijun Li Math 576: Quantitative Risk Management Week 1 1 / 26 #12;Outline see 3 This Course 4 Risk Factors Haijun Li Math 576: Quantitative Risk Management Week 1 2 / 26 #12

  3. Math 576: Quantitative Risk Management lih@math.wsu.edu

    E-Print Network [OSTI]

    Li, Haijun

    Math 576: Quantitative Risk Management Haijun Li lih@math.wsu.edu Department of Mathematics Washington State University Week 12 Haijun Li Math 576: Quantitative Risk Management Week 12 1 / 19 #12;Outline 1 The Point Process Method 2 The Hill Estimation Haijun Li Math 576: Quantitative Risk Management

  4. MATH 576: Quantitative Risk Management (3 credits) Instructor: Haijun Li

    E-Print Network [OSTI]

    Li, Haijun

    MATH 576: Quantitative Risk Management (3 credits) Instructor: Haijun Li Office Locations: Neill: Quantitative Risk Management, Ch 1~7, Princeton University Press, New Jersey Course Purpose This course/computational methods in quantitative risk management. Concepts and methods covered in this course can be applied

  5. Math 576: Quantitative Risk Management lih@math.wsu.edu

    E-Print Network [OSTI]

    Li, Haijun

    Math 576: Quantitative Risk Management Haijun Li lih@math.wsu.edu Department of Mathematics Washington State University Week 6 Haijun Li Math 576: Quantitative Risk Management Week 6 1 / 24 #12;Outline 1 Factor Models 2 Principal Component Analysis Haijun Li Math 576: Quantitative Risk Management Week

  6. Quantitative Verification: Models, Techniques and Tools Marta Kwiatkowska

    E-Print Network [OSTI]

    Oxford, University of

    verification is an analogous technique for establishing quantitative properties of a system modelQuantitative Verification: Models, Techniques and Tools Marta Kwiatkowska Oxford University of the state-transition graph of the model and is therefore more powerful than test- ing. Quantitative

  7. Quantitative Genetics, Genomics, and the Future of Plant Breeding

    E-Print Network [OSTI]

    Walsh, Bruce

    Quantitative Genetics, Genomics, and the Future of Plant Breeding Bruce Walsh, University on the age of genomics, and so it is reasonable to speculate on the implications of both partial and whole genome sequences for quantitative genetics. Likewise, the tools of modern quantitative genetics have been

  8. Page 1 of 11 INTERNATIONAL WORKSHOP ON QUANTITATIVE FINANCE

    E-Print Network [OSTI]

    Jagannatham, Aditya K.

    Page 1 of 11 4th INTERNATIONAL WORKSHOP ON QUANTITATIVE FINANCE December 22nd to 25th , 2012 at IIT a four (4) days International Workshop on Quantitative Finance at IIT Kanpur, Kanpur, INDIA from 22nd December to 25th December 2012. Information about the Quantitative Finance Workshop The 4th International

  9. Genetic and physical mapping of the Treacher Collins syndrome locus with respect to loci in the chromosome 5q3 region

    SciTech Connect (OSTI)

    Jabs, E.W.; Li, Xiang; Coss, C.; Taylor, E. (Johns Hopkins School of Medicine, Baltimore, MD (United States)); Lovett, M. (Univ. of Texas Southwestern Medical Center, San Antonio, TX (United States)); Yamaoka, L.H.; Speer, M.C. (Duke Univ. Medical Center, Durham, NC (United States)); Cadle, R.; Hall, B. (Univ. of Kentucky, Lexington, KY (United States)); Brown, K. (Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY (United States)) (and others)

    1993-10-01

    Treacher Collins syndrome is an autosomal dominant, craniofacial developmental disorder, and its locus (TCOF1) has been mapped to chromosome 5q3. To refine the location of the gene within this region, linkage analysis was performed among the TCOF1 locus and 12 loci (IL9, FGFA, GRL, D5S207, D5S210, D5S376, CSF1R, SPARC, D5S119, D5S209, D5S527, FGFR4) in 13 Treacher Collins syndrome families. The highest maximum lod score was obtained between loci TCOF1 and D5S210 (Z = 10.52; [theta] = 0.02 [+-] 0.07). The best order, IL9-GRL-D5S207/D5S210-CSF1R-SPARC-D5S119, and genetic distances among these loci were determined in the 40 CEPH families by multipoint linkage analysis. YAC clones were used to establish the order of loci, centromere-5[prime]GRL3[prime]-D5S207-D5S210-D5S376-CSF1R-SPARC-D5S119-telomere. By combining known physical mapping data with ours, the order of chromosome 5q3 markers is centomere-IL9-FGFA-5[prime]GRL3[prime]-D5s207-D5S210-D5S376-CSF1R-SPARC-D5S119-D5S209-FGFR4-telomere. Based on this order, haplotype analysis suggests that the TCOF1 locus resides distal CSF1R and proximal to SPARC within a region less than 1 Mb in size. 29 refs., 2 figs., 2 tabs.

  10. Quantitative imaging of turbulent and reacting flows

    SciTech Connect (OSTI)

    Paul, P.H. [Sandia National Laboratories, Livermore, CA (United States)

    1993-12-01

    Quantitative digital imaging, using planar laser light scattering techniques is being developed for the analysis of turbulent and reacting flows. Quantitative image data, implying both a direct relation to flowfield variables as well as sufficient signal and spatial dynamic range, can be readily processed to yield two-dimensional distributions of flowfield scalars and in turn two-dimensional images of gradients and turbulence scales. Much of the development of imaging techniques to date has concentrated on understanding the requisite molecular spectroscopy and collision dynamics to be able to determine how flowfield variable information is encoded into the measured signal. From this standpoint the image is seen as a collection of single point measurements. The present effort aims at realizing necessary improvements in signal and spatial dynamic range, signal-to-noise ratio and spatial resolution in the imaging system as well as developing excitation/detection strategies which provide for a quantitative measure of particular flowfield scalars. The standard camera used for the study is an intensified CCD array operated in a conventional video format. The design of the system was based on detailed modeling of signal and image transfer properties of fast UV imaging lenses, image intensifiers and CCD detector arrays. While this system is suitable for direct scalar imaging, derived quantities (e.g. temperature or velocity images) require an exceptionally wide dynamic range imaging detector. To apply these diagnostics to reacting flows also requires a very fast shuttered camera. The authors have developed and successfully tested a new type of gated low-light level detector. This system relies on fast switching of proximity focused image-diode which is direct fiber-optic coupled to a cooled CCD array. Tests on this new detector show significant improvements in detection limit, dynamic range and spatial resolution as compared to microchannel plate intensified arrays.

  11. Quantitative Outgassing studies in DC Electrical breakdown

    E-Print Network [OSTI]

    Levinsen, Yngve Inntjore; Calatroni, Sergio; Taborelli, Mauro; Wünsch, Walter

    2010-01-01

    Breakdown in the accelerating structures sets an important limit to the performance of the CLIC linear collider. Vacuum degradation and subsequent beam instability are possible outcomes of a breakdown if too much gas is released from the cavity surface. Quantitative data of gas released by breakdowns are provided for copper (milled Cu-OFE, as-received and heat-treated), and molybdenum. These data are produced in a DC spark system based on a capacitance charged at fixed energy, and will serve as a reference for the vacuum design of the CLIC accelerating structures.

  12. Quantitative WDS analysis using electron probe microanalyzer

    SciTech Connect (OSTI)

    Ul-Hamid, Anwar [Research Institute, King Fahd University of Petroleum and Minerals, P.O. Box 1073, Dhahran 31261 (Saudi Arabia)]. E-mail: anwar@kfupm.edu.sa; Tawancy, Hani M. [Research Institute, King Fahd University of Petroleum and Minerals, P.O. Box 1073, Dhahran 31261 (Saudi Arabia); Mohammed, Abdul-Rashid I. [Research Institute, King Fahd University of Petroleum and Minerals, P.O. Box 1073, Dhahran 31261 (Saudi Arabia); Al-Jaroudi, Said S. [Saudi Aramco, P.O. Box 65, Tanajib 31311 (Saudi Arabia); Abbas, Nureddin M. [Research Institute, King Fahd University of Petroleum and Minerals, P.O. Box 1073, Dhahran 31261 (Saudi Arabia)

    2006-04-15

    In this paper, the procedure for conducting quantitative elemental analysis by ZAF correction method using wavelength dispersive X-ray spectroscopy (WDS) in an electron probe microanalyzer (EPMA) is elaborated. Analysis of a thermal barrier coating (TBC) system formed on a Ni-based single crystal superalloy is presented as an example to illustrate the analysis of samples consisting of a large number of major and minor elements. The analysis was performed by known standards and measured peak-to-background intensity ratios. The procedure for using separate set of acquisition conditions for major and minor element analysis is explained and its importance is stressed.

  13. Path to development of quantitative safety goals

    SciTech Connect (OSTI)

    Joksimovic, V.; Houghton, W.J.

    1980-04-01

    There is a growing interest in defining numerical safety goals for nuclear power plants as exemplified by an ACRS recommendation. This paper proposes a lower frequency limit of approximately 10/sup -4//reactor-year for design basis events. Below this frequency, down, to a small frequency such as 10/sup -5//reactor-year, safety margin can be provided by, say, site emergency plans. Accident sequences below 10/sup -5/ should not impact public safety, but it is prudent that safety research programs examine sequences with significant consequences. Once tentatively agreed upon, quantitative safety goals together with associated implementation tools would be factored into regulatory and design processes.

  14. Quantitative genetics of growth, carcass-quality traits, and disease resistance in hybrid striped bass (Morone chrysops [female] x Morone saxatilis [male]) 

    E-Print Network [OSTI]

    Wang, Xiaoxue

    2009-05-15

    (REML) algorithm were used to estimate variance components associated with dam, sire, and dam x sire interaction effects. Dam and sire effect on juvenile growth (weight, length and growth rates) were significant, whereas dam by sire interaction effect...

  15. Quantitative tomographic measurements of opaque multiphase flows

    SciTech Connect (OSTI)

    GEORGE,DARIN L.; TORCZYNSKI,JOHN R.; SHOLLENBERGER,KIM ANN; O'HERN,TIMOTHY J.; CECCIO,STEVEN L.

    2000-03-01

    An electrical-impedance tomography (EIT) system has been developed for quantitative measurements of radial phase distribution profiles in two-phase and three-phase vertical column flows. The EIT system is described along with the computer algorithm used for reconstructing phase volume fraction profiles. EIT measurements were validated by comparison with a gamma-densitometry tomography (GDT) system. The EIT system was used to accurately measure average solid volume fractions up to 0.05 in solid-liquid flows, and radial gas volume fraction profiles in gas-liquid flows with gas volume fractions up to 0.15. In both flows, average phase volume fractions and radial volume fraction profiles from GDT and EIT were in good agreement. A minor modification to the formula used to relate conductivity data to phase volume fractions was found to improve agreement between the methods. GDT and EIT were then applied together to simultaneously measure the solid, liquid, and gas radial distributions within several vertical three-phase flows. For average solid volume fractions up to 0.30, the gas distribution for each gas flow rate was approximately independent of the amount of solids in the column. Measurements made with this EIT system demonstrate that EIT may be used successfully for noninvasive, quantitative measurements of dispersed multiphase flows.

  16. Mapping of genes using a bovine BAC Library to determine their effects on economically important traits in cattle 

    E-Print Network [OSTI]

    Herring, Kimberly Lynn

    1995-01-01

    ). The analyses did not show the genotype at KLHGHM to have an effect on any of the other traits measured (P>.05). Sex of calf was an important source of variation for all traits except REA, MARB and QG. Sire breed by dam breed combinations were significant for BW...

  17. Hand Asymmetry Does Not Relate to Key Life History Traits in Post-Menopausal Contemporary Finnish Women

    E-Print Network [OSTI]

    Helle, Samuli

    Hand Asymmetry Does Not Relate to Key Life History Traits in Post-Menopausal Contemporary Finnish of developmental instability, DI) and life history traits have received a great deal of attention in the non. In humans, only a few studies have related FA to life histories and fitness. In this paper we study

  18. SELF-REFERENTIAL EVALUATIONS OF TRAITS AND VALUES DO NOT RELY ON EPISODIC MEMORY: AN ERP STUDY

    E-Print Network [OSTI]

    Johnson Jr.,, Ray

    SELF-REFERENTIAL EVALUATIONS OF TRAITS AND VALUES DO NOT RELY ON EPISODIC MEMORY: AN ERP STUDY of Psychology, Queens College and The Graduate Center of CUNY DISCUSSION Tasks ERP Session: · Trait evaluation (including 150 ms baseline) Rejection of trials with EOG and other artifacts ERP Recordings INTRODUCTION

  19. A 45-year followup study of breast and other cancers in kindred 107 and linkage analysis of candidate loci

    SciTech Connect (OSTI)

    Goldgar, D.E.; Neuhausen, S.L.; Ward, J.H.

    1994-09-01

    One of the earliest large kindreds with inherited susceptibility to breast cancer was reported by Gardner and Stephens in 1950. This family, denoted K107, was ascertained in 1947 by a genetics student with two great aunts who died of breast cancer in their 40`s. Subsequent clinical and genealogical follow-up identified 7 additional cases of early-onset breast cancer. The family was updated several times, most notably in 1980. For the present study K107 was recently reinvestigated and over 75 blood samples gathered for genotyping. The kindred now contains 38 cases of female breast cancer, 3 cases of male breast cancer, and 6 cases of ovarian cancer, 18 of which have been identified since the 1980 report. Examination of the obligate carriers demonstrates that that gene responsible for the breast and ovarian cancer in K107 is highly penetrant. Other cancers appear to be associated with expression of this gene, most notably prostate cancer, melanoma, and uterine cancer. Linkage to the BRCA1 region in K107 was excluded based upon the analysis of genotypings at four loci covering the BRCA1 gene on chromosome 17q has been excluded in this family, using four highly polymorphic markers in the BRCA1 region (multipoint LOD score -3.27). Eight other candidate breast cancer susceptibility genes and candidate regions, including p53 and ESR have also been tested for linkage and excluded. Studies to formally re-estimate penetrance and test for excesses of all cancer sites and a genomic search in this family are in progress.

  20. Effects of Gossypol Consumption on the Growth Traits of Red Deer Stags and Supplemental Melatonin for Advancement of Estrous Cycles in Red Deer Hinds. 

    E-Print Network [OSTI]

    Morgan, Shane

    2011-08-08

    Experiment I studied the effect of dietary gossypol (G) on antler and body growth traits of red deer stags, whereas Experiment II studied the effect of exogenous melatonin on female red deer reproductive traits. Specifically ...

  1. EVOLUTIONARY ECOLOGY OF PLANT DEFENCES Plant traits that predict resistance to herbivores

    E-Print Network [OSTI]

    Lajeunesse, Marc J.

    the concentrations of secondary metabolites and herbivore susceptibility ­ plant traits other than secondary Carolina 27695, USA Summary 1. Although secondary metabolites are recognized as fundamental to the defence metabolites are the most important anti-herbivore defence of plants. We propose the hypothesis that herbivores

  2. Partitioning of Phenotypic Variance in Life-history Traits in the Great Tit, Parus major

    E-Print Network [OSTI]

    Browne, William J.

    ; (4) A large proportion of the variation seems to be due to effects that may represent the stochastic (+) 207 449 6693 Fax (+) 207 586 1457 Running head: Sources of Phenotypic Variation in Reproductive Traits #12;A characteristic of all individual-based population studies is the large variation between

  3. Belowground traits of herbaceous species in young coniferous forests of the Olympic

    E-Print Network [OSTI]

    Franklin, Jerry

    , Washington Ann L. Lezberg, Joseph A. Antos, and Charles B. Halpern Abstract: Variation in belowground traits, vegetative spread, and proportion of biomass in belowground structures. All species were perennial and most structures. The proportion of total biomass in belowground structures varied considerably among species (21

  4. Heritability, covariation and natural selection on 24 traits of common evening primrose (Oenothera biennis) from

    E-Print Network [OSTI]

    Agrawal, Anurag

    proportion of variation in herbivory (r2 = 0.73). At the same time, selection acted on lifetime biomass, life how functional trait variation influences ecological interactions and adaptation to various envi adaptations to different abiotic environments (Ackerly, 2004; Hemsley & Poole, 2004). Similarly, variation

  5. vol. 166, supplement the american naturalist october 2005 Testosterone in Females: Mediator of Adaptive Traits,

    E-Print Network [OSTI]

    Ketterson, Ellen D.

    of Adaptive Traits, Constraint on Sexual Dimorphism, or Both? E. D. Ketterson,1,* V. Nolan Jr.,1, and M could constrain dimorphism. Results from birds, including the dark-eyed junco (Junco hyemalis), reveal of sexual dimorphism. Keywords: hormonal pleiotropy, correlated responses, sex differences, hormonal

  6. What traits are carried on mobile genetic elements, DJ Rankin1,2

    E-Print Network [OSTI]

    Rankin, Daniel

    REVIEW What traits are carried on mobile genetic elements, and why? DJ Rankin1,2 , EPC Rocha3 of Oxford, Oxford, UK Although similar to any other organism, prokaryotes can transfer genes vertically from Although in all organisms genes are transferred verti- cally from parent to offspring, many microorganisms

  7. Does development mode organize life-history traits in the parasitoid Hymenoptera?

    E-Print Network [OSTI]

    Mayhew, Peter

    Does development mode organize life-history traits in the parasitoid Hymenoptera? PETER J. MAYHEW hypothesis are assessed using life history data from 474 species of parasitoid Hymenoptera. 3. A degree-rich group of insects that share a basic life history characteris- tic: ospring develop by feeding upon

  8. Trait sorting in Daphnia colonising man-made lakes MICHAEL R. ALLEN*,

    E-Print Network [OSTI]

    Cáceres, Carla E.

    . VANDYKE* AND CARLA E. CA´ CERES*, *School of Integrative Biology, University of Illinois, Urbana, IL, U.S.A. Program in Ecology, Evolution and Conservation Biology, University of Illinois, Urbana, IL, U.S.A. Annis and regional distributions of trait variation. Since the formation of the lakes 80 years ago, cladoceran

  9. Association with pathogenic bacteria affects life-history traits and population growth in Caenorhabditis elegans.

    E-Print Network [OSTI]

    Diaz, S. Anaid; Mooring, Eric Q.; Rens, Elisabeth G.; Restif, Olivier

    2015-03-23

    with respect to its association with pathogenic bacteria. In order to start filling the gap between the two areas, we conducted a series of experiments aiming at measuring life-history traits as well as population growth of C. elegans in response to three...

  10. Community Ecology of Algal Biofuels: Complementarity and Trait-Based Approaches

    E-Print Network [OSTI]

    Community Ecology of Algal Biofuels: Complementarity and Trait-Based Approaches Jakob O. Nalley economically viable option for mass cultivation of algae as a biofuel source, such systems face a number principles to address the limi- tations of open-pond cultivation is a promising direction in algal biofuel

  11. Fire Severity Filters Regeneration Traits to Shape Community Assembly in Alaska's Boreal Forest

    E-Print Network [OSTI]

    Ickert-Bond, Steffi

    Fire Severity Filters Regeneration Traits to Shape Community Assembly in Alaska's Boreal Forest pre-fire forest type­ black spruce forests of Interior Alaska. Patterns of community composition Forest Service, Pacific Northwest Research Station, Fairbanks, Alaska, United States of America, 2

  12. Static and dynamic expression of life history traits in the northern fulmar Fulmarus glacialis

    E-Print Network [OSTI]

    Aberdeen, University of

    369 Static and dynamic expression of life history traits in the northern fulmar Fulmarus glacialis and Environmental Sciences, Univ. of Aberdeen, Cromarty, IV11 8YJ, UK. Understanding the static and dynamic of aging and of life histories. We analyzed the statics and dynamics of reproduction and survival in a wild

  13. Elected in 100 milliseconds: Appearance-Based Trait Inferences and Voting

    E-Print Network [OSTI]

    Todorov, Alex

    have largely assumed that voters are rational actors, whose political choices are free from biases Abstract Recent research has shown that rapid judgments about the personality traits of political of political prefer- ences. Finally, we introduce a computer model of face-based competence judgments, which we

  14. Evaluation of carcass traits of F? calves sired by Bos indicus bulls 

    E-Print Network [OSTI]

    Fry, Cody Allen

    1999-01-01

    The carcass traits of 229 F[] steers sired by 15 Brahman bulls and 1 Nellore bull and out of Angus and Hereford dams were evaluated. The first calves were born in the Spring of 1994 and the last calves were born in the Spring of 1998. Of the steers...

  15. American Journal of Botany 89(9): 13881400. 2002. RESPONSES OF CARBON ACQUISITION TRAITS TO

    E-Print Network [OSTI]

    Barrett, Spencer C.H.

    of natural selection than the consequence of constraints imposed by pleiotropy. Key words: androdioecy; gas TO IRRADIANCE AND LIGHT QUALITY IN MERCURIALIS ANNUA (EUPHORBIACEAE): EVIDENCE FOR WEAK INTEGRATION OF PLASTIC It is often suggested that traits will be integrated, either because of pleiotropy or because natural

  16. Maternal Effects on Reproduction and Traits of Reciprocal F1 Dams of

    E-Print Network [OSTI]

    Ma, Lena

    Maternal Effects on Reproduction and Production Traits of Reciprocal F1 Dams of Angus, Brahman to determine if differences in their producing ability can be attributed to the breed of their dam. The purpose of this paper is to compare the performance of reciprocal F1 crossbred females as to dam breed for both

  17. How invader traits interact with resident communities and resource availability to determine invasion success

    E-Print Network [OSTI]

    Haddad, Nick

    149 How invader traits interact with resident communities and resource availability to determine for limited resources is considered a key factor controlling invasion success. Resource availability can be viewed in either the long or short-term. Long-term availability depends on the baseline nutrient

  18. Species traits predict the effects of disturbance and productivity on diversity

    E-Print Network [OSTI]

    Haddad, Nick

    that ­ alone or in concert ­ increasing disturbance intensity or frequency, or decreasing productivity, reducedLETTER SpeciesŐ traits predict the effects of disturbance and productivity on diversity Nick M, University of Colorado, UCB 334, Boulder CO 80309, USA. Abstract Disturbance is an important factor

  19. Xylem traits mediate a trade-off between resistance to freezethaw-induced embolism and photosynthetic

    E-Print Network [OSTI]

    Sack, Lawren

    to vascular transport caused by freeze­thaw-induced embolism. · Freeze­thaw-induced loss in hydraulic embolized following freeze­thaw events (Hacke & Sperry, 2001). Such a loss of water transport can reduceXylem traits mediate a trade-off between resistance to freeze­thaw-induced embolism

  20. Contrasting trait responses in plant communities to experimental and geographic variation in precipitation

    E-Print Network [OSTI]

    Kraft, Nathan

    in precipitation Brody Sandel1,2 , Leah J. Goldstein3 , Nathan J.B. Kraft1,4 , Jordan G. Okie5 , Michal I. Shuldman: climate change, experiments, functional ecology, plant communities, plant functional traits, precipitation. Summary · Patterns of precipitation are likely to change significantly in the coming century

  1. A Database of Lotic Invertebrate Traits for North By Nicole K. M. Vieira, N. LeRoy Poff, Daren M. Carlisle, Stephen R. Moulton II, Marci L. Koski,

    E-Print Network [OSTI]

    Poff, N. LeRoy

    A Database of Lotic Invertebrate Traits for North America By Nicole K. M. Vieira, N. LeRoy Poff, Boris C., 2006, A database of lotic invertebrate traits for North America: U.S. Geological Survey Data ........................................................................................................................................3 Selecting traits for the database

  2. Conducting Quantitative Electrochemistry on a TEM to Study Rate...

    Office of Scientific and Technical Information (OSTI)

    Conducting Quantitative Electrochemistry on a TEM to Study Rate Dependencies in the Reversible Lithiation of Silicon Nanowires. Citation Details In-Document Search Title:...

  3. Quantitative Measurement of Phosphoproteome Response to Osmotic Stress in Arabidopsis

    E-Print Network [OSTI]

    Radivojac, Predrag

    Quantitative Measurement of Phosphoproteome Response to Osmotic Stress in Arabidopsis Based may help shed light on phospho- rylation response to osmotic stress in plants. Molecular & Cellular

  4. Quantitative planar laser-induced fluorescence imaging of multi...

    Office of Scientific and Technical Information (OSTI)

    Article: Quantitative planar laser-induced fluorescence imaging of multi-component fuelair mixing in a firing gasoline-direct-injection engine: Effects of residual exhaust...

  5. Quantitative multi-modal NDT data analysis

    SciTech Connect (OSTI)

    Heideklang, René; Shokouhi, Parisa [Division 8.5, BAM Fed. Institute for Materials Research and Testing, Unter den Eichen 87, 12205 Berlin (Germany)

    2014-02-18

    A single NDT technique is often not adequate to provide assessments about the integrity of test objects with the required coverage or accuracy. In such situations, it is often resorted to multi-modal testing, where complementary and overlapping information from different NDT techniques are combined for a more comprehensive evaluation. Multi-modal material and defect characterization is an interesting task which involves several diverse fields of research, including signal and image processing, statistics and data mining. The fusion of different modalities may improve quantitative nondestructive evaluation by effectively exploiting the augmented set of multi-sensor information about the material. It is the redundant information in particular, whose quantification is expected to lead to increased reliability and robustness of the inspection results. There are different systematic approaches to data fusion, each with its specific advantages and drawbacks. In our contribution, these will be discussed in the context of nondestructive materials testing. A practical study adopting a high-level scheme for the fusion of Eddy Current, GMR and Thermography measurements on a reference metallic specimen with built-in grooves will be presented. Results show that fusion is able to outperform the best single sensor regarding detection specificity, while retaining the same level of sensitivity.

  6. Hydrogen quantitative risk assessment workshop proceedings.

    SciTech Connect (OSTI)

    Groth, Katrina M.; Harris, Aaron P.

    2013-09-01

    The Quantitative Risk Assessment (QRA) Toolkit Introduction Workshop was held at Energetics on June 11-12. The workshop was co-hosted by Sandia National Laboratories (Sandia) and HySafe, the International Association for Hydrogen Safety. The objective of the workshop was twofold: (1) Present a hydrogen-specific methodology and toolkit (currently under development) for conducting QRA to support the development of codes and standards and safety assessments of hydrogen-fueled vehicles and fueling stations, and (2) Obtain feedback on the needs of early-stage users (hydrogen as well as potential leveraging for Compressed Natural Gas [CNG], and Liquefied Natural Gas [LNG]) and set priorities for %E2%80%9CVersion 1%E2%80%9D of the toolkit in the context of the commercial evolution of hydrogen fuel cell electric vehicles (FCEV). The workshop consisted of an introduction and three technical sessions: Risk Informed Development and Approach; CNG/LNG Applications; and Introduction of a Hydrogen Specific QRA Toolkit.

  7. Quantitative analysis of software architectures Simonetta Balsamo*, Marco Bernardo

    E-Print Network [OSTI]

    Balsamo, Simonetta

    life cycle since the early stages. We focus on quantitative analysis of software architectures (SA in the software development life cycle. We consider SA to describe both the structure and the behavior of software and notations to formulate quantitative requirements early in the software life-cycle, and methods that identify

  8. Quantitative Microwave Imaging Based on a Huber regularization

    E-Print Network [OSTI]

    Pizurica, Aleksandra

    Quantitative Microwave Imaging Based on a Huber regularization Funing Bai, Wilfried Philips University, Belgium Abstract--Reconstruction of inhomogeneous dielectric objects from microwave scattering by means of quantitative microwave tomography is a nonlinear, ill-posed inverse problem. In this paper, we

  9. Statistics and Quantitative Risk Management for Banking and In-

    E-Print Network [OSTI]

    Embrechts, Paul

    Statistics and Quantitative Risk Management for Banking and In- surance Paul Embrechts Risk practice Abstract As an emerging field of applied research, Quantitative Risk Management (QRM) poses a lot management. Topics treated include the use of risk measures in regulation, including their statistical

  10. Cell Polarity: Quantitative Modeling as a Tool in Cell Biology

    E-Print Network [OSTI]

    Allard, Jun

    REVIEW Cell Polarity: Quantitative Modeling as a Tool in Cell Biology Alex Mogilner,1 * Jun Allard,1 Roy Wollman2 Among a number of innovative approaches that have modernized cell biology, modeling characterized by varying levels of biological detail and mathematical complexity. We argue that a quantitative

  11. Climate policy and competitiveness: Policy guidance and quantitative evidence

    E-Print Network [OSTI]

    Climate policy and competitiveness: Policy guidance and quantitative evidence Jared C. Carbone NicholasRivers July 2014 Abstract When considering adoption of a domestic climate change policy survey the literature on the quantitative impacts of unilateral climate change policy derived from

  12. Method of quantitating dsDNA

    DOE Patents [OSTI]

    Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)

    2002-01-01

    A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.

  13. Asbestos exposure--quantitative assessment of risk

    SciTech Connect (OSTI)

    Hughes, J.M.; Weill, H.

    1986-01-01

    Methods for deriving quantitative estimates of asbestos-associated health risks are reviewed and their numerous assumptions and uncertainties described. These methods involve extrapolation of risks observed at past relatively high asbestos concentration levels down to usually much lower concentration levels of interest today--in some cases, orders of magnitude lower. These models are used to calculate estimates of the potential risk to workers manufacturing asbestos products and to students enrolled in schools containing asbestos products. The potential risk to workers exposed for 40 yr to 0.5 fibers per milliliter (f/ml) of mixed asbestos fiber type (a permissible workplace exposure limit under consideration by the Occupational Safety and Health Administration (OSHA) ) are estimated as 82 lifetime excess cancers per 10,000 exposed. The risk to students exposed to an average asbestos concentration of 0.001 f/ml of mixed asbestos fiber types for an average enrollment period of 6 school years is estimated as 5 lifetime excess cancers per one million exposed. If the school exposure is to chrysotile asbestos only, then the estimated risk is 1.5 lifetime excess cancers per million. Risks from other causes are presented for comparison; e.g., annual rates (per million) of 10 deaths from high school football, 14 from bicycling (10-14 yr of age), 5 to 20 for whooping cough vaccination. Decisions concerning asbestos products require participation of all parties involved and should only be made after a scientifically defensible estimate of the associated risk has been obtained. In many cases to date, such decisions have been made without adequate consideration of the level of risk or the cost-effectiveness of attempts to lower the potential risk. 73 references.

  14. System and method for leveraging human physiological traits to control microprocessor frequency

    SciTech Connect (OSTI)

    Shye, Alex; Pan, Yan; Scholbrock, Benjamin; Miller, J. Scott; Memik, Gokhan; Dinda, Peter A; Dick, Robert P

    2014-03-25

    A system and method for leveraging physiological traits to control microprocessor frequency are disclosed. In some embodiments, the system and method may optimize, for example, a particular processor-based architecture based on, for example, end user satisfaction. In some embodiments, the system and method may determine, for example, whether their users are satisfied to provide higher efficiency, improved reliability, reduced power consumption, increased security, and a better user experience. The system and method may use, for example, biometric input devices to provide information about a user's physiological traits to a computer system. Biometric input devices may include, for example, one or more of the following: an eye tracker, a galvanic skin response sensor, and/or a force sensor.

  15. Differences among Beefmaster sires in their offspring in birth, growth and carcass traits 

    E-Print Network [OSTI]

    Fehr, Harry Bartel

    2002-01-01

    were 37.38 ± 0.26 kg, 220.40 ± 1.68 kg, 398.57 ± 7.03 kg, and 471.46 ± 8.89 kg, respectively. The carcass traits averaged 290.38 ± 2.36 kg, 74.175 ± .624 cm˛, 2.51 ± 0.04 %, 525.90 ± 6.91, 3.22 ± 0.06, respectively, for hot carcass weight, longissimus...

  16. Effects of Residual Feed Intake Classification on Temperament, Carcass Composition, and Feeding Behavior Traits in Growing Santa Gertrudis Heifers 

    E-Print Network [OSTI]

    Ramirez, Justin A

    2014-04-16

    Objectives of this study were to evaluate the associations of feed intake, feeding behavior, temperament, and carcass composition with performance and feed efficiency traits in growing heifers. Santa Gertrudis heifers (n ...

  17. THE RELATIONSHIP OF FEED EFFICIENCY WITH PERFORMANCE, ULTRASOUND, CARCASS AND NON-CARCASS TRAITS IN BEEF CATTLE 

    E-Print Network [OSTI]

    Ribeiro, Flavio

    2010-07-14

    and to examine the relationships with growth, ultrasound, carcass, non-carcass, and tenderness traits in two studies involving Santa Gertrudis (n = 114) steers, and Angus bulls (n = 16) and heifers (n = 16). In both experiments, RFI was calculated...

  18. Qualitative Reasoning Feeding Back into Quantitative Model-Based Tracking

    E-Print Network [OSTI]

    Nebel, Bernhard

    Qualitative Reasoning Feeding Back into Quantitative Model-Based Tracking Christian K¨ohler, 1- bilize basic signal processing and pattern recognition processes like the reliable extraction of some

  19. Qualitative Reasoning Feeding Back into Quantitative ModelBased Tracking

    E-Print Network [OSTI]

    Nebel, Bernhard

    Qualitative Reasoning Feeding Back into Quantitative Model­Based Tracking Christian KË? ohler, 1­ bilize basic signal processing and pattern recognition processes like the reliable extraction of some

  20. Quantitative Evaluation of Passage Retrieval Algorithms for Question

    E-Print Network [OSTI]

    Tellex, Stefanie

    , USA #12;Road Map Overview of factoid question answering. Our experiments: a quantitative in taxes and $1,000 for permits ``does not send the right signal'' to film producers who might be Document

  1. Quantitative Characterization of Filament Dynamics by Single-Molecule Lifetime

    E-Print Network [OSTI]

    Needleman, Daniel

    CHAPTER 29 Quantitative Characterization of Filament Dynamics by Single-Molecule Lifetime University, Cambridge, Massachusetts 02138 Abstract I. Introduction to Cytoskeletal Filament Dynamics II III. Theoretical Foundations A. Equivalence to the First-Passage Time Problem B. Models of Filament

  2. Quantitative NDA Measurements of Advanced Reprocessing Product Materials Containing U, NP, PU, and AM 

    E-Print Network [OSTI]

    Goddard, Braden

    2013-04-05

    of this first principle technique have been identified: (1) quantitative measurement of uranium, neptunium, plutonium, and americium materials; (2) quantitative measurement of mixed oxide (MOX) materials; (3) quantitative measurement of uranium materials; and (4...

  3. Influence of Bull Traits and Bull to Female Ratio on Reproductive Perfromance in Beef Females and of Nutrition During Gestation on Calving Difficulty in Primiparous Beef Females 

    E-Print Network [OSTI]

    Bloomberg, Blake David

    2011-08-08

    trait across all 3 years.. 36 4 Repeatablity (r) of physical and reproductive traits of bulls pre- and post- breeding season??????????????... 36 5 Mean + SE heifer traits at initiation of trial... difference could have very complex results. Nutrition Extremely low energy intake beginning early in life can delay puberty (Bratton et al., 1959; VanDemark and Mauger, 1964) and, if severe enough, can permanently impair sperm output (VanDemark et al...

  4. Characterization of Feeding Behavior Traits and Associations with Performance and Feed Efficiency in Finishing Beef Cattle 

    E-Print Network [OSTI]

    Mendes, Egleu Diomedes Marinho

    2011-10-21

    ., 2000). Many studies have examined the relationships between feeding behavior traits and feed efficiency in poultry (Van Eerden et al., 2004), swine (de Haer et al., 1993; Rauw et al., 2006a, b; Von Felde et al., 1996), sheep (Cammack et al., 2005.... (2007); eRauw et al. (2006a); fRauw et al. (2006b); gRobinson and Oddy (2004); hSchwartzkopf-Genswein et al. (2002); iVon Felde et al. (1996) Bold values are correlated with P < 0.05 There were no specification for the P-value for the phenotypic...

  5. Influence of Inflammatory Polyarthritis on Quantitative Heel Ultrasound Measurements

    E-Print Network [OSTI]

    Pye, Stephen R; Marshall, Tarnya; Gaffney, Karl; Luben, Robert; Khaw, Kay-Tee; Silman, Alan J; Symmons, Deborah PM; O'Neill, Terence W

    2012-07-26

    Dis 1995, 54:801–806. 9. Lane NE, Pressman AR, Star VL, Cummings SR, Nevitt MC, Study of Osteoporotic Fractures Research Group: Rheumatoid arthritis and bone mineral density in elderly women. J Bone Miner Res 1995, 10:257–263. 10. Deodhar AA, Woolf AD... in men and women by quantitative ultrasound of the calcaneus: EPIC-Norfolk prospective population study. Lancet 2004, 363:197–202. 17. Bauer DC, Ewing SK, Cauley JA, Ensrud KE, Cummings SR, Orwoll ES, et al: Quantitative ultrasound predicts hip and non...

  6. Quantitative Metrics of Social Response for Autism Diagnosis*

    E-Print Network [OSTI]

    Scassellati, Brian

    Quantitative Metrics of Social Response for Autism Diagnosis* Brian Scassellati Department and treatment of autism, a pervasive developmental disorder which results in selective impairment of social to address critical problems in diagnosing autism. We provide preliminary data and observations on how

  7. Characterizing Google Hacking: A First Large-Scale Quantitative Study

    E-Print Network [OSTI]

    Gu, Guofei

    Characterizing Google Hacking: A First Large-Scale Quantitative Study Jialong Zhang, Jayant Notani.com Abstract. Google Hacking continues to be abused by attackers to find vulnerable websites on current the characteristics of vulnerabilities targeted by Google Hacking (e.g., what kind of vulnerabilities are typically

  8. Quantitative Imaging of Corrosion in Plates by Eddy Current Methods

    E-Print Network [OSTI]

    Santosa, Fadil

    Quantitative Imaging of Corrosion in Plates by Eddy Current Methods Bruno Luong \\Lambda and Fadil the problem of determining material loss due to cor­ rosion in a plate. Eddy current data are collected on one the relationship between eddy current loop impedance change with the loss profile. In this context, we show

  9. Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular

    E-Print Network [OSTI]

    Tian, Weidong

    the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity and quantitative proteomes of hepatocellular car- cinoma (HCC). The effects of three different histochemical stains and contaminating proteins, e.g. blood proteins. Several ap- proaches have been developed to resolve those problems

  10. 50TH ANNIVERSARY Quantitative correlation of interfacial contamination

    E-Print Network [OSTI]

    Florida, University of

    50TH ANNIVERSARY Quantitative correlation of interfacial contamination and antiphase domain Science+Business Media New York 2015 Abstract The role of interfacial contamination on anti- phase domain surface contamination across the wafer. APB density in the as-grown GaAs film was examined with the aid

  11. Quantitative Thermo-acoustics and related problems Guillaume Bal

    E-Print Network [OSTI]

    Biasutti, Michela

    Quantitative Thermo-acoustics and related problems Guillaume Bal Department of Applied Physics of Mathematics, University of Washington, Seattle, WA 98195 E-mail: tzhou@math.washington.edu Abstract. Thermo-acoustic in tissues with the good resolution properties of ultrasounds. Thermo-acoustic imaging may be decomposed

  12. Supplemental Information Translating HIV sequences into quantitative fitness landscapes to

    E-Print Network [OSTI]

    Ferguson, Andrew

    Supplemental Information Translating HIV sequences into quantitative fitness landscapes to predict contact information. Tel: (617) 253-3890. Fax: (617) 253-2272. Email: arupc@mit.edu. #12;Supplemental Inventory 1. Supplemental Figures and Tables Figure S1, Related to Figure 2 Figure S2, Related to Figure 4

  13. Qualitative vs. Quantitative Plan Diversity in Case-Based Planning

    E-Print Network [OSTI]

    Muñoz-Avila, Héctor

    Qualitative vs. Quantitative Plan Diversity in Case-Based Planning Alexandra Coman, Héctor Muñoz 18015 {alc308, hem4}@lehigh.edu Abstract. Plan diversity has practical value in multiple planning domains, including travel planning, military planning and game planning. Existing methods for obtaining

  14. A Quantitative Analysis of Developer Information Needs in Software Ecosystems

    E-Print Network [OSTI]

    Jäger, Gerhard

    A Quantitative Analysis of Developer Information Needs in Software Ecosystems Nicole Haenni, Mircea popular tools developers use to satisfy their ecosystem-related information needs. Categories and Subject present the results of an investigation into the nature of information needs of software developers who

  15. Lessons Learned from Quantitative Dynamical Modeling in Systems Biology

    E-Print Network [OSTI]

    Timmer, Jens

    Lessons Learned from Quantitative Dynamical Modeling in Systems Biology Andreas Raue1,2 *. , Marcel of Physics, University of Freiburg, Freiburg, Germany, 2 Institute of Computational Biology, Helmholtz Center, Munich, Germany, 3 Systems Biology of Signal Transduction, German Cancer Research Center

  16. Quantitative Analysis for Controllability of Symmetric Control Systems

    E-Print Network [OSTI]

    Murota, Kazuo

    Quantitative Analysis for Controllability of Symmetric Control Systems Reiko Tanaka 1 and Kazuo with the controllability of systems possessing symmetries. In partic­ ular, we aim to give a lower bound for the number of functioning modules needed to ensure the controllability of the entire symmetric system. Our concern

  17. On Some Constructions in Quantitative Domain Theory (Extended Abstract)

    E-Print Network [OSTI]

    Spreen, Dieter

    On Some Constructions in Quantitative Domain Theory (Extended Abstract) Dieter Spreen Theoretische of an approximation, the theory of approximation based on domains was mainly of a qualitative nature. The situation introduced by K. Martin in his thesis [7]. They are strongly intertwined with the topological structure

  18. The Role of Statistical Principles in Quantitative Biomedical Modeling

    E-Print Network [OSTI]

    Davidian, Marie

    The Role of Statistical Principles in Quantitative Biomedical Modeling Atlantic Coast Conference University http://www.stat.ncsu.edu/davidian Statistical Principles in Biomedical Modeling 1 #12;Outline 1. Modeling in biomedical research 2. Sources of variation in data 3. Statistical models 4. Hierarchical

  19. Informatics methods to enable sharing of quantitative imaging research data,

    E-Print Network [OSTI]

    Rubin, Daniel L.

    reuse of quantitative imaging data in the community. Methods: We performed a survey of the current tools online at www.sciencedirect.com Magnetic Resonance Imaging 30 (2012) 1249­1256 Disclosures of conflict of interest: Mia Levy, none; John Freymann, none; Justin Kirby, none; Andriy Fedorov, Ph.D., none; Fiona

  20. Genomics Populations for Characterization of Economically Important Traits Structured populations of beef cattle with extensive phenotypic records for economical-

    E-Print Network [OSTI]

    Genomics Populations for Characterization of Economically Important Traits Structured populations efficiency and carcass merit, are a critical need in the post-genome sequencing era. Coupled with the availability of the 7.5x assembly of the bovine genome sequence and the single nucleotide polymorphisms (SNP

  1. A Trait Specific Model of GM Crop Adoption among U.S. Corn Farmers in the Upper Midwest

    E-Print Network [OSTI]

    Foltz, Jeremy D.

    to already existing seeds. This makes genetically modified (GM) seeds different from standard technological. Introduction The advent of genetic engineering techniques has transformed how scientists can manipulate. In this case GM seeds involve adding specific traits into a plant in a manner similar to how a food company

  2. Making the best of the worst of times: traits underlying combined shade and drought tolerance of Ruscus aculeatus

    E-Print Network [OSTI]

    Sack, Lawren

    woodland areas of the Mediterranean Basin and southern Europe. The combined drought and shade tolerance on a detailed trait survey for Ruscus aculeatus L. and Ruscus microglossum Bertol., focusing on gas exchange, low shoot hydraulic conductance, low cuticular conductance, and substantial water storage tissue

  3. http://www.jstor.org Natural Selection on Floral Traits in Two Species of Lobelia with Different Pollinators

    E-Print Network [OSTI]

    Johnston, Mark

    http://www.jstor.org Natural Selection on Floral Traits in Two Species of Lobelia with Different and Conditions of Use, available at http://www.jstor.org/page/info/about/policies/terms.jsp. JSTOR's Terms the discovery and use of these resources. For more information about JSTOR, please contact support

  4. Selection on Crop-Derived Traits and QTL in Sunflower (Helianthus annuus) Crop-Wild Hybrids under Water

    E-Print Network [OSTI]

    Burke, John M.

    Selection on Crop-Derived Traits and QTL in Sunflower (Helianthus annuus) Crop-Wild Hybrids under grown under wild-like low water conditions. Crop-derived petiole length and head diameter were favored size and leaf pressure potential. Interestingly, the additive effect of the crop-derived allele

  5. High-throughput behavioral phenotyping of drug and alcohol susceptibility traits in the expanded panel of BXD recombinant inbred strains

    SciTech Connect (OSTI)

    Philip, Vivek M [ORNL; Ansah, T [University of Tennessee Health Science Center, Memphis; Blaha, C, [University of Tennessee Health Science Center, Memphis; Cook, Melloni N. [University of Memphis; Hamre, Kristin M. [University of Tennessee Health Science Center, Memphis; Lariviere, William R [University of Pittsburgh; Matthews, Douglas B [Baylor University; Goldowitz, Daniel [University of British Columbia, Vancouver; Chesler, Elissa J [ORNL

    2010-01-01

    Genetic reference populations, particularly the BXD recombinant inbred strains, are a valuable resource for the discovery of the bio-molecular substrates and genetic drivers responsible for trait variation and co- ariation. This approach can be profitably applied in the analysis of susceptibility and mechanisms of drug and alcohol use disorders for which many predisposing behaviors may predict occurrence and manifestation of increased preference for these substances. Many of these traits are modeled by common mouse behavioral assays, facilitating the detection of patterns and sources of genetic co-regulation of predisposing phenotypes and substance consumption. Members of the Tennessee Mouse Genome Consortium have obtained behavioral phenotype data from 260 measures related to multiple behavioral assays across several domains: self-administration, response to, and withdrawal from cocaine, MDMA, morphine and alcohol; novelty seeking; behavioral despair and related neurological phenomena; pain sensitivity; stress sensitivity; anxiety; hyperactivity; and sleep/wake cycles. All traits have been measured in both sexes and the recently expanded panel of 69 additional BXD recombinant inbred strains (N=69). Sex differences and heritability estimates were obtained for each trait, and a comparison of early (N = 32) and recent BXD RI lines was performed. Primary data is publicly available for heritability, sex difference and genetic analyses using www.GeneNetwork.org. These analyses include QTL detection and genetic analysis of gene expression. Stored results from these analyses are available at http://ontologicaldiscovery.org for comparison to other genomic analysis results. Together with the results of related studies, these data form a public resource for integrative systems genetic analysis of neurobehavioral traits.

  6. Review of progress in quantitative NDE. [Nondestructive Evaluation (NDE)

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    This booklet is composed of abstracts from papers submitted at a meeting on quantitative NDE. A multitude of topics are discussed including analysis of composite materials, NMR uses, x-ray instruments and techniques, manufacturing uses, neural networks, eddy currents, stress measurements, magnetic materials, adhesive bonds, signal processing, NDE of mechanical structures, tomography,defect sizing, NDE of plastics and ceramics, new techniques, optical and electromagnetic techniques, and nonlinear techniques. (GHH)

  7. A Quantitative Analysis of IRAS Maps of Molecular Clouds

    E-Print Network [OSTI]

    Jennifer J. Wiseman; Fred C. Adams

    1994-04-20

    We present an analysis of IRAS maps of five molecular clouds: Orion, Ophiuchus, Perseus, Taurus, and Lupus. For the classification and description of these astrophysical maps, we use a newly developed technique which considers all maps of a given type to be elements of a pseudometric space. For each physical characteristic of interest, this formal system assigns a distance function (a pseudometric) to the space of all maps; this procedure allows us to measure quantitatively the difference between any two maps and to order the space of all maps. We thus obtain a quantitative classification scheme for molecular clouds. In this present study we use the IRAS continuum maps at 100$\\mu$m and 60$\\mu$m to produce column density (or optical depth) maps for the five molecular cloud regions given above. For this sample of clouds, we compute the ``output'' functions which measure the distribution of density, the distribution of topological components, the self-gravity, and the filamentary nature of the clouds. The results of this work provide a quantitative description of the structure in these molecular cloud regions. We then order the clouds according to the overall environmental ``complexity'' of these star forming regions. Finally, we compare our results with the observed populations of young stellar objects in these clouds and discuss the possible environmental effects on the star formation process. Our results are consistent with the recently stated conjecture that more massive stars tend to form in more ``complex'' environments.

  8. Quantitative EDS Analysis of Nanometer-Scale Core/Shell Pd/Rh...

    Office of Scientific and Technical Information (OSTI)

    Quantitative EDS Analysis of Nanometer-Scale CoreShell PdRh Structures. Citation Details In-Document Search Title: Quantitative EDS Analysis of Nanometer-Scale CoreShell PdRh...

  9. Synthesis and Hardware Implementation of an Unmanned Aerial Vehicle Automatic Landing System Utilizing Quantitative Feedback Theory 

    E-Print Network [OSTI]

    Woodbury, Timothy Daniel

    2014-07-08

    that the control synthesis process using Quantitative Feedback Theory provides robust controllers with generally adequate performance, based on simulation and hardware results. The Quantitative Feedback Theory framework provides a good method for synthesizing...

  10. Quantitative Visualization of ChIP-chip Data by Using Linked...

    Office of Scientific and Technical Information (OSTI)

    Quantitative Visualization of ChIP-chip Data by Using Linked Views Citation Details In-Document Search Title: Quantitative Visualization of ChIP-chip Data by Using Linked Views...

  11. Food choices for hungry broiler breeders: do they prefer quantitative or qualitative dietary restriction? 

    E-Print Network [OSTI]

    Buckley, Louise Anne

    2012-06-30

    This programme of research uses choice test methodologies to quantify hungry broiler breeder chickens’ preferences for qualitative or quantitative dietary restriction. It begins with an outline of quantitative dietary ...

  12. Quantitative Determination of Nanoscale Electronic Properties of Semiconductor Surfaces by Scanning Tunnelling Spectroscopy

    E-Print Network [OSTI]

    Feenstra, Randall

    Quantitative Determination of Nanoscale Electronic Properties of Semiconductor Surfaces by Scanning semiconductor surfaces permits quantitative evaluation of nanoscale electronic properties of the surface. Band properties associated with particular point defects within the material. An overview of the methods employed

  13. QUANTITATIVE EVALUATION OF LEFT VENTRICULAR ROTATIONAL MECHANICS USING MAGNETIC RESONANCE IMAGING

    E-Print Network [OSTI]

    Wang, Zhe

    2015-01-01

    qualitative and quantitative assessment of left ventricular (LV) myocardial deformation in both research and clinical settings. This thesis

  14. Quantitative imaging of freezing at the millimeter scale using neutron radiography

    E-Print Network [OSTI]

    Deinert, Mark

    Quantitative imaging of freezing at the millimeter scale using neutron radiography A.J. Gilbert, M change Quantitative radiography a b s t r a c t Freezing of fluids in capillary structures affects the capability of quantitative imaging of freezing at $3 mm scale. In this study, a 1.6 mm diameter water column

  15. Quantitative Finance, Vol. 12, No. 1, January 2012, 1114 Financial engineering at Columbia University

    E-Print Network [OSTI]

    Hone, James

    Quantitative Finance, Vol. 12, No. 1, January 2012, 11­14 Financial engineering at Columbia of quantitative finance, including derivative securi- ties, risk management, portfolio optimization, trading. Photographs by Mark Broadie. Quantitative Finance ISSN 1469­7688 print/ISSN 1469­7696 online ß 2012 Taylor

  16. Universit de Montral valuation Qualitative et Quantitative d'Outils de Visualisation Logicielle

    E-Print Network [OSTI]

    Keller, Rudolf K.

    #12;Université de Montréal Évaluation Qualitative et Quantitative d'Outils de Visualisation Montréal Faculté des Études Supérieures Ce mémoire intitulé: Évaluation Qualitative et Quantitative d are not aware of any quantitative investigation and survey of SV tools. In this thesis, firstly, we identified

  17. Lect 12: Evolution at multiple loci Evolution at multiple loci

    E-Print Network [OSTI]

    variation VP = VG + VE Heritability = Vp VG Measures proportion of VP due to VG "Broad sense heritability · Continuous variation, polygenic control, small effect of each Mendelian locus · Models of natural selection.06 S = 10.06 - 9.47 S = 0.59 nonbreeders breeders Fig. 8.15 Evolutionary response? #12;Phenotypic variation

  18. Trait variation within a species determines warming responses at different scales

    SciTech Connect (OSTI)

    Karve, Abhijit A [ORNL; Weston, David [ORNL; Souza, Lara [University of Tennessee, Knoxville (UTK); Classen, Aimee T [University of Tennessee, Knoxville (UTK)

    2011-01-01

    Plant species, and the traits associated with them, can help buffer ecosystems to environmental perturbations. Few studies have examined whether within species variation, both among and within populations, can similarly buffer ecosystems to environmental perturbations, such as climatic warming, across levels of organization. Using a dominant plant species in the eastern US, Solidago altissima, we examined whether genotypes of the same species from both southern and northern latitude populations exhibited differential short-term responses to temperature at the cell, leaf, and plant level. At the cell level we quantified the production of reactive oxygen species (by-product of temperature stress) and total oxygen radical antioxidant capacity (which ameliorates temperature stress by-products). At the leaf and plant levels, we measured CO2 assimilation. Increasing temperatures had strong negative impacts on plant-level carbon gain, but weak impacts on cell-level antioxidant capacity. Southern latitude genotypes had greater total antioxidant capacity, but lower leaf-level carbon gain, than did northern genotypes under elevated temperature. At the plant level, northern and southern genotypes exhibited similar declines in carbon gain under elevated temperature, likely because total plant leaf area was higher for southern genotypes than northern genotypes, which compensated for their lower per unit area leaf-level carbon gain. Overall, short-term temperature-induced declines in carbon gain at the plant level may scale to reduce within species variation, both across and within populations, potentially altering ecosystem carbon cycling.

  19. Understanding Beef Cattle Efficiency: I) Understanding Physiological and Digestive Factors Affecting Residual Feed Intake and II) Tannin Supplementation: Effects on Animal Performance, Fermentation, and Carcass Traits 

    E-Print Network [OSTI]

    Krueger, Wimberley K.

    2010-10-12

    no effect on animal performance and efficiency, ruminal fermentation VFA concentrations, MPA, or ammonia concentrations in finishing beef steers. There was also no detrimental effect of tannins on carcass traits; however, hydrolysable tannin supplementation...

  20. An examination of personality, emotional intelligence, coping, gender and subjective well-being with perceived stress (trait and state) in undergraduate students. 

    E-Print Network [OSTI]

    Osborne, Shona Elizabeth

    2009-07-03

    This multivariate study aimed to further understand student stress. Associations between personality, emotional intelligence, coping and subjective well-being with perceived stress (trait and state) were examined in 238 ...

  1. Interpersonal traits and the technology acceptance model: applying the interpersonal circumplex model as a nomological net for understanding user perceptions within human-to-computer interaction 

    E-Print Network [OSTI]

    Brown, Houghton Gregory

    2009-05-15

    This study examines the effects that individual personality traits have on technology acceptance. Previous research on technology acceptance focuses primarily on exogenous variables such as trustor’s perceptions, attitudes, ...

  2. Designer Sorghum Combining the High Digestibility and Waxy Grain Traits of Sorghum for Improved Nutrition Bioethanol Beer Feed and Food Products 

    E-Print Network [OSTI]

    Jampala, Babitha

    2012-07-16

    SORGHUM COMBINING THE HIGH DIGESTIBILITY AND WAXY GRAIN TRAITS IN SORGHUM FOR IMPROVED NUTRITION BIOETHANOL BEER FEED AND FOOD PRODUCTS A Dissertation by BABITHA JAMPALA Submitted to the Office of Graduate Studies of Texas A&M University... in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY May 2012 Major Subject: Plant Breeding DESIGNER SORGHUM COMBINING THE HIGH DIGESTIBILITY AND WAXY GRAIN TRAITS IN SORGHUM FOR IMPROVED NUTRITION BIOETHANOL BEER...

  3. Quantitative infrared absorption cross sections of isoprene for atmospheric measurements

    SciTech Connect (OSTI)

    Brauer, Carolyn S.; Blake, Thomas A.; Guenther, Alex B.; Sharpe, Steven W.; Sams, Robert L.; Johnson, Timothy J.

    2014-11-19

    The OH- and O3- initiated oxidations of isoprene, which is one of the primary volatile organic compounds produced by vegetation, are a major source of atmospheric formaldehyde and other oxygenated organics, yet little quantitative IR data exists for isoprene. We thus report absorption coefficients and integrated band intensities for isoprene in the 600 - 6500 cm-1 region. The pressure-broadened (1 atmosphere N2) spectra were recorded at 278, 298 and 323 K in a 19.96 cm path length cell at 0.112 cm-1 resolution, using a Bruker 66V FTIR. Composite spectra are derived from a minimum of seven pressures at each temperature.

  4. Quantitative Monte Carlo-based holmium-166 SPECT reconstruction

    SciTech Connect (OSTI)

    Elschot, Mattijs; Smits, Maarten L. J.; Nijsen, Johannes F. W.; Lam, Marnix G. E. H.; Zonnenberg, Bernard A.; Bosch, Maurice A. A. J. van den; Jong, Hugo W. A. M. de [Department of Radiology and Nuclear Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht (Netherlands); Viergever, Max A. [Image Sciences Institute, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht (Netherlands)] [Image Sciences Institute, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht (Netherlands)

    2013-11-15

    Purpose: Quantitative imaging of the radionuclide distribution is of increasing interest for microsphere radioembolization (RE) of liver malignancies, to aid treatment planning and dosimetry. For this purpose, holmium-166 ({sup 166}Ho) microspheres have been developed, which can be visualized with a gamma camera. The objective of this work is to develop and evaluate a new reconstruction method for quantitative {sup 166}Ho SPECT, including Monte Carlo-based modeling of photon contributions from the full energy spectrum.Methods: A fast Monte Carlo (MC) simulator was developed for simulation of {sup 166}Ho projection images and incorporated in a statistical reconstruction algorithm (SPECT-fMC). Photon scatter and attenuation for all photons sampled from the full {sup 166}Ho energy spectrum were modeled during reconstruction by Monte Carlo simulations. The energy- and distance-dependent collimator-detector response was modeled using precalculated convolution kernels. Phantom experiments were performed to quantitatively evaluate image contrast, image noise, count errors, and activity recovery coefficients (ARCs) of SPECT-fMC in comparison with those of an energy window-based method for correction of down-scattered high-energy photons (SPECT-DSW) and a previously presented hybrid method that combines MC simulation of photopeak scatter with energy window-based estimation of down-scattered high-energy contributions (SPECT-ppMC+DSW). Additionally, the impact of SPECT-fMC on whole-body recovered activities (A{sup est}) and estimated radiation absorbed doses was evaluated using clinical SPECT data of six {sup 166}Ho RE patients.Results: At the same noise level, SPECT-fMC images showed substantially higher contrast than SPECT-DSW and SPECT-ppMC+DSW in spheres ?17 mm in diameter. The count error was reduced from 29% (SPECT-DSW) and 25% (SPECT-ppMC+DSW) to 12% (SPECT-fMC). ARCs in five spherical volumes of 1.96–106.21 ml were improved from 32%–63% (SPECT-DSW) and 50%–80% (SPECT-ppMC+DSW) to 76%–103% (SPECT-fMC). Furthermore, SPECT-fMC recovered whole-body activities were most accurate (A{sup est}= 1.06 × A ? 5.90 MBq, R{sup 2}= 0.97) and SPECT-fMC tumor absorbed doses were significantly higher than with SPECT-DSW (p = 0.031) and SPECT-ppMC+DSW (p = 0.031).Conclusions: The quantitative accuracy of {sup 166}Ho SPECT is improved by Monte Carlo-based modeling of the image degrading factors. Consequently, the proposed reconstruction method enables accurate estimation of the radiation absorbed dose in clinical practice.

  5. Quantitative Electron Nanodiffraction. (Technical Report) | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTech ConnectSpeedingConnect Pulse energy measurement athemorrhagicAnodes.Quantitative

  6. A quantitative method for measuring the quality of history matches

    SciTech Connect (OSTI)

    Shaw, T.S.; Knapp, R.M.

    1997-08-01

    History matching can be an efficient tool for reservoir characterization. A {open_quotes}good{close_quotes} history matching job can generate reliable reservoir parameters. However, reservoir engineers are often frustrated when they try to select a {open_quotes}better{close_quotes} match from a series of history matching runs. Without a quantitative measurement, it is always difficult to tell the difference between a {open_quotes}good{close_quotes} and a {open_quotes}better{close_quotes} matches. For this reason, we need a quantitative method for testing the quality of matches. This paper presents a method for such a purpose. The method uses three statistical indices to (1) test shape conformity, (2) examine bias errors, and (3) measure magnitude of deviation. The shape conformity test insures that the shape of a simulated curve matches that of a historical curve. Examining bias errors assures that model reservoir parameters have been calibrated to that of a real reservoir. Measuring the magnitude of deviation assures that the difference between the model and the real reservoir parameters is minimized. The method was first tested on a hypothetical model and then applied to published field studies. The results showed that the method can efficiently measure the quality of matches. It also showed that the method can serve as a diagnostic tool for calibrating reservoir parameters during history matching.

  7. Single beam Fourier transform digital holographic quantitative phase microscopy

    SciTech Connect (OSTI)

    Anand, A. Chhaniwal, V. K.; Mahajan, S.; Trivedi, V.; Faridian, A.; Pedrini, G.; Osten, W.; Dubey, S. K.; Javidi, B.

    2014-03-10

    Quantitative phase contrast microscopy reveals thickness or height information of a biological or technical micro-object under investigation. The information obtained from this process provides a means to study their dynamics. Digital holographic (DH) microscopy is one of the most used, state of the art single-shot quantitative techniques for three dimensional imaging of living cells. Conventional off axis DH microscopy directly provides phase contrast images of the objects. However, this process requires two separate beams and their ratio adjustment for high contrast interference fringes. Also the use of two separate beams may make the system more vulnerable to vibrations. Single beam techniques can overcome these hurdles while remaining compact as well. Here, we describe the development of a single beam DH microscope providing whole field imaging of micro-objects. A hologram of the magnified object projected on to a diffuser co-located with a pinhole is recorded with the use of a commercially available diode laser and an arrayed sensor. A Fourier transform of the recorded hologram directly yields the complex amplitude at the image plane. The method proposed was investigated using various phase objects. It was also used to image the dynamics of human red blood cells in which sub-micrometer level thickness variation were measurable.

  8. Theoretical Sensitivity Analysis for Quantitative Operational Risk Management

    E-Print Network [OSTI]

    Kato, Takashi

    2011-01-01

    We study an asymptotic behaviour of the difference between value-at-risks VaR(L) and VaR(L+S) for heavy-tailed random variables L and S as an application to sensitivity analysis of quantitative operational risk management in the framework of an advanced measurement approach (AMA) of Basel II. We have different types of results according to the magnitude relationship of thickness of tails of L and S. Especially if the tail of S is enough thinner than the one of L, then VaR(L + S) - VaR(L) is asymptotically equivalent to an expected loss of S when L and S are independent. We also give some generalized results without the assumption of independence.

  9. Towards quantitative prediction of proteasomal digestion patterns of proteins

    E-Print Network [OSTI]

    Goldobin, Denis S

    2014-01-01

    We discuss the problem of proteasomal degradation of proteins. Though proteasomes are important for all aspects of the cellular metabolism, some details of the physical mechanism of the process remain unknown. We introduce a stochastic model of the proteasomal degradation of proteins, which accounts for the protein translocation and the topology of the positioning of cleavage centers of a proteasome from first principles. For this model we develop the mathematical description based on a master-equation and techniques for reconstruction of the cleavage specificity inherent to proteins and the proteasomal translocation rates, which are a property of the proteasome specie, from mass spectroscopy data on digestion patterns. With these properties determined, one can quantitatively predict digestion patterns for new experimental set-ups. Additionally we design an experimental set-up for a synthetic polypeptide with a periodic sequence of amino acids, which enables especially reliable determination of translocation ...

  10. Towards quantitative prediction of proteasomal digestion patterns of proteins

    E-Print Network [OSTI]

    Denis S. Goldobin; Alexey Zaikin

    2008-06-16

    We discuss the problem of proteasomal degradation of proteins. Though proteasomes are important for all aspects of the cellular metabolism, some details of the physical mechanism of the process remain unknown. We introduce a stochastic model of the proteasomal degradation of proteins, which accounts for the protein translocation and the topology of the positioning of cleavage centers of a proteasome from first principles. For this model we develop the mathematical description based on a master-equation and techniques for reconstruction of the cleavage specificity inherent to proteins and the proteasomal translocation rates, which are a property of the proteasome specie, from mass spectroscopy data on digestion patterns. With these properties determined, one can quantitatively predict digestion patterns for new experimental set-ups. Additionally we design an experimental set-up for a synthetic polypeptide with a periodic sequence of amino acids, which enables especially reliable determination of translocation rates.

  11. Quantitative analytical model for magnetic reconnection in hall magnetohydrodynamics

    SciTech Connect (OSTI)

    Simakov, Andrei N

    2008-01-01

    Magnetic reconnection is of fundamental importance for laboratory and naturally occurring plasmas. Reconnection usually develops on time scales which are much shorter than those associated with classical collisional dissipation processes, and which are not fully understood. While such dissipation-independent (or 'fast') reconnection rates have been observed in particle and Hall magnetohydrodynamics (MHD) simulations and predicted analytically in electron MHD, a quantitative analytical theory of fast reconnection valid for arbitrary ion inertial lengths d{sub i} has been lacking. Here we propose such a theory without a guide field. The theory describes two-dimensional magnetic field diffusion regions, provides expressions for the reconnection rates, and derives a formal criterion for fast reconnection in terms of dissipation parameters and di. It also demonstrates that both open X-point and elongated diffusion regions allow dissipation-independent reconnection and reveals a possibility of strong dependence of the reconnection rates on d{sub i}.

  12. Quantitative Methods for Comparing Different HVAC Control Schemes

    E-Print Network [OSTI]

    Aswani, Anil; Taneja, Jay; Krioukov, Andrew; Culler, David; Tomlin, Claire

    2012-01-01

    Experimentally comparing the energy usage and comfort characteristics of different controllers in heating, ventilation, and air-conditioning (HVAC) systems is difficult because variations in weather and occupancy conditions preclude the possibility of establishing equivalent experimental conditions across the order of hours, days, and weeks. This paper is concerned with defining quantitative metrics of energy usage and occupant comfort, which can be computed and compared in a rigorous manner that is capable of determining whether differences between controllers are statistically significant in the presence of such environmental fluctuations. Experimental case studies are presented that compare two alternative controllers (a schedule controller and a hybrid system learning-based model predictive controller) to the default controller in a building-wide HVAC system. Lastly, we discuss how our proposed methodology may also be able to quantify the efficiency of other building automation systems.

  13. Quantitative validation of PEDFLOW for description of unidirectional pedestrian dynamics

    E-Print Network [OSTI]

    Zhang, J; Chraibi, M; Loehner, R; Haug, E; Gawenat, B

    2015-01-01

    The results of a systematic quantitative validation of PEDFLOW based on the experimental data from FZJ are presented. Unidirectional flow experiments, totaling 28 different combinations with varying entry, corridor and exit widths, were considered. The condition imposed on PEDFLOW was that all the cases should be run with the same input parameters. The exit times and fundamental diagrams for the measuring region were evaluated and compared. This validation process led to modifications and enhancements of the model underlying PEDFLOW. The preliminary conclusions indicate that the results agree well for densities smaller than 3 m-2 and a good agreement is observed even at high densities for the corridors with bcor = 2.4 m, and bcor = 3.0 m. For densities between 1 and 2 m-2 the specific flow and velocities are underpredicted by PEDFLOW.

  14. Quantitative analysis of forest island pattern in selected Ohio landscapes

    SciTech Connect (OSTI)

    Bowen, G.W.; Burgess, R.L.

    1981-07-01

    The purpose of this study was to quantitatively describe the various aspects of regional distribution patterns of forest islands and relate those patterns to other landscape features. Several maps showing the forest cover of various counties in Ohio were selected as representative examples of forest patterns to be quantified. Ten thousand hectare study areas (landscapes) were delineated on each map. A total of 15 landscapes representing a wide variety of forest island patterns was chosen. Data were converted into a series of continuous variables which contained information pertinent to the sizes, shape, numbers, and spacing of woodlots within a landscape. The continuous variables were used in a factor analysis to describe the variation among landscapes in terms of forest island pattern. The results showed that forest island patterns are related to topography and other environmental features correlated with topography.

  15. Quantitative genetic activity graphical profiles for use in chemical evaluation

    SciTech Connect (OSTI)

    Waters, M.D.; Stack, H.F.; Garrett, N.E.; Jackson, M.A.

    1990-12-31

    A graphic approach, terms a Genetic Activity Profile (GAP), was developed to display a matrix of data on the genetic and related effects of selected chemical agents. The profiles provide a visual overview of the quantitative (doses) and qualitative (test results) data for each chemical. Either the lowest effective dose or highest ineffective dose is recorded for each agent and bioassay. Up to 200 different test systems are represented across the GAP. Bioassay systems are organized according to the phylogeny of the test organisms and the end points of genetic activity. The methodology for producing and evaluating genetic activity profile was developed in collaboration with the International Agency for Research on Cancer (IARC). Data on individual chemicals were compiles by IARC and by the US Environmental Protection Agency (EPA). Data are available on 343 compounds selected from volumes 1-53 of the IARC Monographs and on 115 compounds identified as Superfund Priority Substances. Software to display the GAPs on an IBM-compatible personal computer is available from the authors. Structurally similar compounds frequently display qualitatively and quantitatively similar profiles of genetic activity. Through examination of the patterns of GAPs of pairs and groups of chemicals, it is possible to make more informed decisions regarding the selection of test batteries to be used in evaluation of chemical analogs. GAPs provided useful data for development of weight-of-evidence hazard ranking schemes. Also, some knowledge of the potential genetic activity of complex environmental mixtures may be gained from an assessment of the genetic activity profiles of component chemicals. The fundamental techniques and computer programs devised for the GAP database may be used to develop similar databases in other disciplines. 36 refs., 2 figs.

  16. Natural bacterial communities serve as quantitative geochemical biosensors

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Smith, Mark B.; Rocha, Andrea M.; Smillie, Chris S.; Olesen, Scott W.; Paradis, Charles; Wu, Liyou; Campbell, James H.; Fortney, Julian L.; Mehlhorn, Tonia L.; Lowe, Kenneth A.; et al

    2015-05-12

    Biological sensors can be engineered to measure a wide range of environmental conditions. Here we show that statistical analysis of DNA from natural microbial communities can be used to accurately identify environmental contaminants, including uranium and nitrate at a nuclear waste site. In addition to contamination, sequence data from the 16S rRNA gene alone can quantitatively predict a rich catalogue of 26 geochemical features collected from 93 wells with highly differing geochemistry characteristics. We extend this approach to identify sites contaminated with hydrocarbons from the Deepwater Horizon oil spill, finding that altered bacterial communities encode a memory of prior contamination,more »even after the contaminants themselves have been fully degraded. We show that the bacterial strains that are most useful for detecting oil and uranium are known to interact with these substrates, indicating that this statistical approach uncovers ecologically meaningful interactions consistent with previous experimental observations. Future efforts should focus on evaluating the geographical generalizability of these associations. Taken as a whole, these results indicate that ubiquitous, natural bacterial communities can be used as in situ environmental sensors that respond to and capture perturbations caused by human impacts. These in situ biosensors rely on environmental selection rather than directed engineering, and so this approach could be rapidly deployed and scaled as sequencing technology continues to become faster, simpler, and less expensive. Here we show that DNA from natural bacterial communities can be used as a quantitative biosensor to accurately distinguish unpolluted sites from those contaminated with uranium, nitrate, or oil. These results indicate that bacterial communities can be used as environmental sensors that respond to and capture perturbations caused by human impacts.« less

  17. Variation in, inheritance of, and correlations between a number of growth and form traits in three sycamore populations 

    E-Print Network [OSTI]

    Nebgen, Russell James

    1980-01-01

    to evaluate different breeding strategies. Genet1c correlations and the coeff1cient of genetic pred1ction (CGp) prov1de informat1on as to how selection for one train w111 affect other important traits. For example, selection for volume could lower wood...milar results 1n two natural stands at Yucatan and Catfish Point, Mississippi . In a 7-year old sycamore plantation, Sauc1er and Ike (1969) exam1ned specific gravities for the first three rings following dif- ferent fertilizer applications (applied...

  18. 11.220 Quantitative Reasoning and Statistical Method for Planning I, Spring 2006

    E-Print Network [OSTI]

    Zegras, P. Christopher

    This course develops logical, empirically based arguments using statistical techniques and analytic methods. It covers elementary statistics, probability, and other types of quantitative reasoning useful for description, ...

  19. Quantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG Emissions

    Office of Energy Efficiency and Renewable Energy (EERE)

    Plenary V: Biofuels and Sustainability: Acknowledging Challenges and Confronting MisconceptionsQuantitative Analysis of Biofuel Sustainability, Including Land Use Change GHG EmissionsJennifer B....

  20. The Trauma Story: A Qualitative and Quantitative Exploration of Iraqi Survivors' Experiences 

    E-Print Network [OSTI]

    Shoeb, Marwa H

    2006-01-01

    Qualitative and Quantitative Exploration of Iraqi Survivors’ Experiences by Marwa Hossam fShoeb J B.S. (Brown University) 2002 A thesis

  1. Quantitative evaluation of general corrosion of Type 304 stainless steel in subcritical and

    E-Print Network [OSTI]

    Benning, Liane G.

    Quantitative evaluation of general corrosion of Type 304 stainless steel in subcritical the corrosion rate of Type 304 stainless steel (SS) in subcritical and supercritical environments. The EN

  2. Quantitative dynamic modeling of transcriptional networks of embryonic stem cells using integrated framework of Pareto optimality and energy balance

    E-Print Network [OSTI]

    Avila, Marco A., Ph. D. Massachusetts Institute of Technology

    2009-01-01

    Embryonic Stem Cells (ESCs) are pluripotent and thus are considered the "cell type of choice". ESCs exhibit several phenotypic traits (e.g., proliferation, differentiation, apoptosis, necrosis, etc.) and when differentiated ...

  3. Quantitation of RNA with RiboGreenTM Stain using the The quantitation of RNA samples is a prerequisite for many different techniques in

    E-Print Network [OSTI]

    Raizada, Manish N.

    as reverse transcriptase PCR (RT-PCR), and differential display PCR. Traditionally, quantitation of RNA RNA ranging from 0 to 10 µg/ml with a 4-parameter logistic best fit to describe the data. (B

  4. Quantitative infrared absorption cross sections of isoprene for atmospheric measurements

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Brauer, C. S.; Blake, T. A.; Guenther, A. B.; Sharpe, S. W.; Sams, R. L.; Johnson, T. J.

    2014-11-19

    Isoprene (C5H8, 2-methyl-1,3-butadiene) is a volatile organic compound (VOC) and is one of the primary contributors to annual global VOC emissions. Isoprene is produced primarily by vegetation as well as anthropogenic sources, and its OH- and O3-initiated oxidations are a major source of atmospheric oxygenated organics. Few quantitative infrared studies have been reported for isoprene, limiting the ability to quantify isoprene emissions via remote or in situ infrared detection. We thus report absorption cross sections and integrated band intensities for isoprene in the 600–6500 cm-1 region. The pressure-broadened (1 atmosphere N2) spectra were recorded at 278, 298, and 323 Kmore »in a 19.94 cm path-length cell at 0.112 cm-1 resolution, using a Bruker IFS 66v/S Fourier transform infrared (FTIR) spectrometer. Composite spectra are derived from a minimum of seven isoprene sample pressures, each at one of three temperatures, and the number densities are normalized to 296 K and 1 atm.« less

  5. Comprehensive Quantitative Analysis of Ovarian and Breast Cancer Tumor Peptidomes

    SciTech Connect (OSTI)

    Xu, Zhe; Wu, Chaochao; Xie, Fang; Slysz, Gordon W.; Tolic, Nikola; Monroe, Matthew E.; Petyuk, Vladislav A.; Payne, Samuel H.; Fujimoto, Grant M.; Moore, Ronald J.; Fillmore, Thomas L.; Schepmoes, Athena A.; Levine, Douglas; Townsend, Reid; Davies, Sherri; Li, Shunqiang; Ellis, Matthew; Boja, Emily; Rodriguez, Henry; Rodland, Karin D.; Liu, Tao; Smith, Richard D.

    2015-01-01

    Aberrant degradation of proteins is associated with many pathological states, including cancers. Mass spectrometric analysis of tumor peptidomes, the intracellular and intercellular products of protein degradation, has the potential to provide biological insights on proteolytic processing in cancer. However, attempts to use the information on these smaller protein degradation products from tumors for biomarker discovery and cancer biology studies have been fairly limited to date, largely due to the lack of effective approaches for robust peptidomics identification and quantification, and the prevalence of confounding factors and biases associated with sample handling and processing. Herein, we have developed an effective and robust analytical platform for comprehensive analyses of tissue peptidomes, and which is suitable for high throughput quantitative studies. The reproducibility and coverage of the platform, as well as the suitability of clinical ovarian tumor and patient-derived breast tumor xenograft samples with post-excision delay of up to 60 min before freezing for peptidomics analysis, have been demonstrated. Moreover, our data also show that the peptidomics profiles can effectively separate breast cancer subtypes, reflecting tumor-associated protease activities. Peptidomics complements results obtainable from conventional bottom-up proteomics, and provides insights not readily obtainable from such approaches.

  6. STATISTICAL APPLICATIONS TO QUANTITATIVE SEISMIC RESERVOIR CHARACTERIZATION AND MONITORING AT WEST PEARL QUEEN

    E-Print Network [OSTI]

    STATISTICAL APPLICATIONS TO QUANTITATIVE SEISMIC RESERVOIR CHARACTERIZATION AND MONITORING AT WEST to characterize reservoirs from seismic data more quantitatively. This thesis describes a methodology to quantify in the reservoir characterization and monitoring of CO2 sequestration at West Pearl Queen Field, ear Hobbs, New

  7. Quantitative analysis of infrared contrast enhancement algorithms Seth Weith-Glushko1

    E-Print Network [OSTI]

    Salvaggio, Carl

    , and quantitatively evaluate infrared contrast enhancement algorithms for use in a real-time long-wave infrared imageQuantitative analysis of infrared contrast enhancement algorithms Seth Weith-Glushko1 and Carl range imagery output from current sensors are compressed in a pleasing way for display on lower dynamic

  8. A Mathematical Programming Model for Optimal Layout Considering Quantitative Risk Analysis

    E-Print Network [OSTI]

    Grossmann, Ignacio E.

    A Mathematical Programming Model for Optimal Layout Considering Quantitative Risk Analysis Nancy risk analysis; Plant layout *Corresponding author. Phone: +52-461-611-7575 Ext. 5577. E-mail: arturo of plant layout with safety considerations. The model considers a quantitative risk analysis to take safety

  9. Four dimensional visualization of highly transient fuel sprays by microsecond quantitative x-ray tomography

    E-Print Network [OSTI]

    Gruner, Sol M.

    -resolved quantitative fuel distribution allowed a realistic numerical fluid dynamic simulation with initial conditions- timal for probing the fuel, a blend of a calibration fluid and a cerium-containing fuel additiveFour dimensional visualization of highly transient fuel sprays by microsecond quantitative x

  10. Long-Term Data Maintenance in Wide-Area Storage Systems: A Quantitative Approach

    E-Print Network [OSTI]

    Kubiatowicz, John D.

    Long-Term Data Maintenance in Wide-Area Storage Systems: A Quantitative Approach Hakim Weatherspoon-Term Data Maintenance in Wide-Area Storage Systems: A Quantitative Approach Hakim Weatherspoon, Byung data replication levels is a fundamental process of wide-area storage systems; replicas must be created

  11. Fuel Cell Generation in Geo-Distributed Cloud Services: A Quantitative Study

    E-Print Network [OSTI]

    Li, Baochun

    Fuel Cell Generation in Geo-Distributed Cloud Services: A Quantitative Study Zhi Zhou1 Fangming Liu of fuel cell energy in cloud computing, yet it is unclear what and how much benefit it may bring. This paper, for the first time, attempts to quantitatively examine the benefits brought by fuel cell

  12. FRE 385/585 Quantitative Methods for Business and Resource Management

    E-Print Network [OSTI]

    Custom Course Publication: Quantitative Methods for Business and Natural Resource Management, FRE 385FRE 385/585 Quantitative Methods for Business and Resource Management University of British Columbia Fall, 2014 Instructor: Michael Johnson Contact Info: Email: mjohnson@mail.ubc.ca Phone: tba Office

  13. Abstract--Quantitative volumetric measurement and feature analysis for various clinical applications require image

    E-Print Network [OSTI]

    Abstract--Quantitative volumetric measurement and feature analysis for various clinical of a region of interest, which reflects the shape characteristics and volumetric variation of the target or deterministic solution to the PV effect, and its successful implementation could improve quantitative volumetric

  14. PCFG based on quantitative representation of AA properties proved to be successful for PS00219

    E-Print Network [OSTI]

    Nebel, Jean-Christophe

    alphabet, we use quantitative properties of amino acids to reduce the number of symbols. Our techniqueConclusion § PCFG based on quantitative representation of AA properties proved to be successful relationships between amino acids (AA) § Probabilistic Context-Free Grammar (PCFG) is a more powerful language

  15. Effects of Residual Feed Intake Classification on Feed Efficiency, Feeding Behavior, Carcass Traits, and Net Revenue in Angus-Based Composite Steers 

    E-Print Network [OSTI]

    Walter, Joel

    2012-02-14

    and carcass measurements on between-animal variation in net revenue of feedlot steers. Performance, feed intake and feeding behavior traits were measured in 508 Angus-based composite steers, using the GrowSafe feed-intake measurement system, while fed a high...

  16. Homogeneity of the Genetic Variance-Covariance Matrix for Antipredator Traits in Two Natural Populations of the Garter Snake Thamnophis ordinoides

    E-Print Network [OSTI]

    Brodie III, Edmund D.

    Homogeneity of the Genetic Variance-Covariance Matrix for Antipredator Traits in Two Natural's Terms and Conditions of Use, available at http://www.jstor.org/page/info/about/policies/terms.jsp. JSTOR the discovery and use of these resources. For more information about JSTOR, please contact support

  17. Laboratory Experiment 1. Calibration of Volumetric Glassware An important trait of a good analyst is the ability to extract the best possible data from his

    E-Print Network [OSTI]

    Nazarenko, Alexander

    Laboratory Experiment 1. Calibration of Volumetric Glassware An important trait of a good analyst to calibrate your own volumetric glassware (burettes, pipettes, flasks, etc.) to measure the exact volumes delivered or contained. Volumetric glassware can be calibrated by measuring the mass of water they contain

  18. SusLabNWE: Integrating qualitative and quantitative data to understand people’s everyday energy behavior

    E-Print Network [OSTI]

    Lockton, Dan; Bowden, Flora; Greene, Catherine; Brass, Clare; Gheerawo, Rama

    2013-01-01

    making alongside their actual energy data, in the processwith quantitative energy data, will allow us to design and

  19. Machine Learning Enhancement of Storm-Scale Ensemble Probabilistic Quantitative Precipitation Forecasts

    E-Print Network [OSTI]

    Xue, Ming

    Machine Learning Enhancement of Storm-Scale Ensemble Probabilistic Quantitative Precipitation uncertainty. Machine learning methods can produce calibrated probabilistic forecasts from the raw ensemble and machine learning are working to address these challenges. Numerical weather prediction (NWP) models

  20. Copyright 2003 by the Genetics Society of America Quantitative Classification and Natural Clustering of Caenorhabditis elegans

    E-Print Network [OSTI]

    Schafer, William R.

    Copyright 2003 by the Genetics Society of America Quantitative Classification and Natural'saccessibility to germline transformation, these animals movements over long time periods and save digital im-are highly

  1. Applying petroleum geophysics to astrophysics: Quantitative 4D seismic study of the solar interior

    E-Print Network [OSTI]

    Crawford, Ian

    Applying petroleum geophysics to astrophysics: Quantitative 4D seismic study, is to a significant extent based on tomographic methods developed in geophysics. The proposed time-lapse seismic methods developed in petroleum geophysics offer the next

  2. Evaluation of a modified quadruple energy window scatter subtraction algorithm for quantitative imaging with In-111 

    E-Print Network [OSTI]

    Sadler,John J.

    2000-01-01

    Accurate quantitative imaging for radionuclide uptake in tumors and organs is an important challenge in radioimmunotherapy. Inaccurate determination of uptake could adversely impact the subsequent therapy dose. An overestimation of activity could...

  3. Combined confocal Raman and quantitative phase microscopy system for biomedical diagnosis

    E-Print Network [OSTI]

    Kang, Jeon Woong

    We have developed a novel multimodal microscopy system that incorporates confocal Raman, confocal reflectance, and quantitative phase microscopy (QPM) into a single imaging entity. Confocal Raman microscopy provides detailed ...

  4. The Impact of Age on Quantitative Infrared Pupillometry in Healthy Children 1-18 Years

    E-Print Network [OSTI]

    Brown, Jacob

    2014-05-31

    OBJECTIVE: The goal of this study was to establish normative values under ambient light conditions for measurements of quantitative pupillometry in children. METHODS: This was a cross-sectional analysis of pupillometry ...

  5. Atomic Force Microscopy of Photosystem II and Its Unit Cell Clustering Quantitatively Delineate the Mesoscale

    E-Print Network [OSTI]

    Geissler, Phillip

    the Mesoscale Variability in Arabidopsis Thylakoids Bibiana Onoa1 , Anna R. Schneider2 , Matthew D. Brooks3 Quantitatively Delineate the Mesoscale Variability in Arabidopsis Thylakoids. PLoS ONE 9(7): e101470. doi:10

  6. Quantitative genetic analysis of growth and survival in Penaeus vannamei versus temperature 

    E-Print Network [OSTI]

    De Tomas Kutz, Alvaro J

    1998-01-01

    Two 8-week laboratory experiments were conducted to evaluate quantitative genetic aspects of growth and survival in juveniles of the marine shrimp Penaeus vannamei versus temperature. Experiment I involved 4,486 progeny ...

  7. DSS-mediated Inhibition of Quantitative Real-time Polymerase Chain Reaction 

    E-Print Network [OSTI]

    Kaur, Jaspreet

    2011-08-04

    The incidence of inflammatory bowel diseases (IBD), including ulcerative colitis (UC), is on the rise. UC is modeled in rodents by the consumption of dextran sodium sulfate (DSS). Quantitative real-time polymerase chain reaction (qPCR) is a...

  8. Syllabus for BE700 / PY895, Fall 2014 "Methods and Logic in Quantitative Biology"

    E-Print Network [OSTI]

    Vajda, Sandor

    Biology is in the midst of a transformation into a fully quantitative, theory-rich science. For example: 893-5. Week of 9/15: All-or-none phenomena (bistability and "the digital paradigm") - · Novick A

  9. Revised process for work zone decision-making based on quantitative performance measures 

    E-Print Network [OSTI]

    Hartmann, Thomas Wayne

    2008-10-10

    Work zones create one of the most challenging environments for drivers. Implementing work zones on urban freeways creates many issues, especially with respect to mobility. Decisions made regarding the work zone should be informed by quantitative...

  10. Quantitative Mapping of Protein Structure by Hydroxyl Radical Footprinting-Mediated Structural Mass Spectrometry: A Protection

    E-Print Network [OSTI]

    Yang, Sichun

    Article Quantitative Mapping of Protein Structure by Hydroxyl Radical Footprinting, Case Western Reserve University, Cleveland, Ohio ABSTRACT Measurements from hydroxyl radical features of a protein can be comprehensively probed by hydroxyl radical footprinting (HRF) mediated

  11. A quantitative assessment of nuclear weapons proliferation risk utilizing probabilistic methods

    E-Print Network [OSTI]

    Sentell, Dennis Shannon, 1971-

    2002-01-01

    A comparative quantitative assessment is made of the nuclear weapons proliferation risk between various nuclear reactor/fuel cycle concepts using a probabilistic method. The work presented details quantified proliferation ...

  12. Quantitative Brain Electrical Activity in the Initial Screening of Mild Traumatic Brain Injuries

    E-Print Network [OSTI]

    O'Neil, Brian; Prichep, Leslie S.; Naunheim, Roseanne; Chabot, Robert

    2012-01-01

    J, et al. Use of Brain Electrical 32. Slobounov S, Cao C,device measuring brain electrical activity can be used inet al Quantitative Brain Electrical Activity in Screening of

  13. Direct and quantitative broadband absorptance micro/nano spectroscopy using FTIR and bilayer cantilever probes

    E-Print Network [OSTI]

    Hsu, Wei-Chun

    2012-01-01

    Optical properties of micro/nano materials are important for many applications in biology, optoelectronics, and energy. In this thesis, a method is described to directly measure the quantitative absorptance spectra of ...

  14. A step towards quantitative lipoprotein density profiling analysis: applied Rayleigh scattering 

    E-Print Network [OSTI]

    Nowlin, Michael

    2009-05-15

    is imperative in assessing risk factors accurately. Light scattering techniques, primarily Rayleigh scattering, are applied to density separated serum samples in resulting in improved qualitative data with progress in quantitative measurements through imaging...

  15. The development of processing methods for a quantitative histological investigation of rat hearts 

    E-Print Network [OSTI]

    Jetton, Emily Hope

    2004-11-15

    In order to understand the mechanical functions of the cardiac muscle it is important to first understand the microstructure of the tissue. Young et al. (1998) realized that quantitative three-dimensional information about the ventricular...

  16. Multi-scale quantitative elastography and its application to blood pressure estimation

    E-Print Network [OSTI]

    Zakrzewski, Aaron Michael

    2013-01-01

    Elastography is a method that can be used to measure the elasticity of soft biological tissue and, ultimately, to detect cancerous tumors. In this thesis, quantitative compression based ultrasound elastography is developed ...

  17. Lung Extraction, Lobe Segmentation and Hierarchical Region Assessment for Quantitative Analysis on High

    E-Print Network [OSTI]

    Lung Extraction, Lobe Segmentation and Hierarchical Region Assessment for Quantitative Analysis Care Division, Brigham and Women's Hospital, Boston, MA Abstract. Regional assessment of lung disease specific to different lung regions on high resolution computed tomography (HRCT) datasets. We present

  18. The Application of Fluorescence Lifetime Imaging Microscopy to Quantitatively Map Mixing and Temperature in Microfluidic Systems 

    E-Print Network [OSTI]

    Graham, Emmelyn M

    2008-01-01

    The technique of Fluorescence Lifetime Imaging Microscopy (FLIM) has been employed to quantitatively and spatially map the fluid composition and temperature within microfluidic systems. A molecular probe with a ...

  19. Distributed quantitative precipitation forecasts combining information from radar and numerical weather prediction model outputs

    E-Print Network [OSTI]

    Ganguly, Auroop Ratan

    2002-01-01

    Applications of distributed Quantitative Precipitation Forecasts (QPF) range from flood forecasting to transportation. Obtaining QPF is acknowledged to be one of the most challenging areas in hydrology and meteorology. ...

  20. Qualitative and Quantitative Management Tools Used by Financial Officers in Public Research Universities 

    E-Print Network [OSTI]

    Trexler, Grant 1961-

    2012-11-16

    This dissertation set out to identify effective qualitative and quantitative management tools used by financial officers (CFOs) in carrying out their management functions of planning, decision making, organizing, staffing, communicating, motivating...

  1. Automated Quantitative Analysis of Capnogram Shape for COPD–Normal and COPD–CHF Classification

    E-Print Network [OSTI]

    Mieloszyk, Rebecca J.

    We develop an approach to quantitative analysis of carbon dioxide concentration in exhaled breath, recorded as a function of time by capnography. The generated waveform – or capnogram – is currently used in clinical practice ...

  2. Quantitative evaluation of wrist posture and typing performance: A comparative study of 4 computer keyboards

    SciTech Connect (OSTI)

    Burastero, S.

    1994-05-01

    The present study focuses on an ergonomic evaluation of 4 computer keyboards, based on subjective analyses of operator comfort and on a quantitative analysis of typing performance and wrist posture during typing. The objectives of this study are (1) to quantify differences in the wrist posture and in typing performance when the four different keyboards are used, and (2) to analyze the subjective preferences of the subjects for alternative keyboards compared to the standard flat keyboard with respect to the quantitative measurements.

  3. Quantitative and qualitative evaluation of the aerobic and facultative bacterial flora of voided canine urine 

    E-Print Network [OSTI]

    Biggerstaff, Jane

    1978-01-01

    QUANTITATIVE AND QUALITATIVE EVALUATION OF THE AEROBIC AND FACULTATIVE BACTERIAL FLORA OF VOIDED CANINE URINE A Thesis by JANE BIGGERSTAFF Submitted to the Graduate College of Texas ARM University in partial fulfillment of the requirements... and content by Chairman of gs}mmittoe) (Head of Department) (Nember (Nember) August 1978 443163 ABSTRACT Quantitative and Qualitative Evaluation of the Aerobic and Facultative Bacterial Flora of Voided Canine Urine. (August, 1978) Jane Biggerstaff, B...

  4. Quantitative determination of mineral composition by powder x-ray diffraction

    DOE Patents [OSTI]

    Pawloski, G.A.

    1984-08-10

    An external standard intensity ratio method is used for quantitatively determining mineralogic compositions of samples by x-ray diffraction. The method uses ratios of x-ray intensity peaks from a single run. Constants are previously determined for each mineral which is to be quantitatively measured. Ratios of the highest intensity peak of each mineral to be quantified in the sample and the highest intensity peak of a reference mineral contained in the sample are used to calculate sample composition.

  5. Genomics Mechanisms of Carbon Allocation and Partitioning in Poplar

    SciTech Connect (OSTI)

    Kirst, Matias; Peter, Gary; Martin, Timothy

    2009-07-30

    The genetic control of carbon allocation and partitioning in woody perennial plants is poorly understood despite its importance for carbon sequestration. It is also unclear how environmental cues such as nitrogen availability impact the genes that regulate growth, and biomass allocation and wood composition in trees. To address these questions we phenotyped 396 clonally replicated genotypes of an interspecific pseudo-backcross pedigree of Populus for wood composition and biomass traits in above and below ground organs. The loci that regulate growth, carbon allocation and partitioning under two nitrogen conditions were identified, defining the contribution of environmental cues to their genetic control. Fifty-seven quantitative trait loci (QTL) were identified for twenty traits analyzed. The majority of QTL are specific to one of the two nitrogen treatments, demonstrating significant nitrogen-dependent genetic control. A highly significant genetic correlation was observed between plant growth and lignin/cellulose composition, and QTL co-localization identified the genomic position of potential pleiotropic regulators. Gene expression analysis of all poplar genes was also characterized in differentiating xylem, whole-roots and developing leaves of 192 of the segregating population. By integrating the QTL and gene expression information we identified genes that regulate carbon partitioning and several biomass growth related properties. The work developed in this project resulted in the publication of three book chapters, four scientific articles (three others currently in preparation), 17 presentations in international conferences and two provisional patent applications.

  6. Can autistic traits present in the normal population determine social communication abilities? An investigation into correlations between AQ score and performance in communication tasks testing audience design and lexical alignment. 

    E-Print Network [OSTI]

    Baring, Rosalind M B

    2009-07-03

    This study explored the concept of the Autistic Spectrum (Wing (1988)), and the theory that autistic traits of varying severity can be identified and measured in the general population using Baron-Cohen, Wheelwright, ...

  7. Identification and genetic characterization of maize cell wall variation for improved biorefinery feedstock characteristics

    SciTech Connect (OSTI)

    Pauly, Markus [UC Berkeley] [UC Berkeley; Hake, Sarah [USDA Albany] [USDA Albany

    2013-10-31

    The objectives of this program are to 1) characterize novel maize mutants with altered cell walls for enhanced biorefinery characteristics and 2) find quantitative trait loci (QTLs) related to biorefinery characteristics by taking advantage of the genetic diversity of maize. As a result a novel non-transgenic maize plant (cal1) has been identified, whose stover (leaves and stalk) contain more glucan in their walls leading to a higher saccharification yield, when subjected to a standard enzymatic digestion cocktail. Stacking this trait with altered lignin mutants yielded evene higher saccharification yields. Cal-1 mutants do not show a loss of kernel and or biomass yield when grown in the field . Hence, cal1 biomass provides an excellent feedstock for the biofuel industry.

  8. A model system for QTL analysis: Effects of alcohol dehydrogenase genotype on alcohol pharmacokinetics

    SciTech Connect (OSTI)

    Martin, N.G.; Nightingale, B.; Whitfield, J.B.

    1994-09-01

    There is much interest in the detection of quantitative trait loci (QTL) - major genes which affect quantitative phenotypes. The relationship of polymorphism at known alcohol metabolizing enzyme loci to alcohol pharmacokinetics is a good model system. The three class I alcohol dehydrogenase genes are clustered on chromosome 4 and protein electrophoresis has revealed polymorphisms at the ADH2 and ADH3 loci. While different activities of the isozymes have been demonstrated in vitro, little work has been done in trying to relate ADH polymorphism to variation in ethanol metabolism in vivo. We previously measured ethanol metabolism and psychomotor reactivity in 206 twin pairs and demonstrated that most of the repeatable variation was genetic. We have now recontacted the twins to obtain DNA samples and used PCR with allele specific primers to type the ADH2 and ADH3 polymorphisms in 337 individual twins. FISHER has been used to estimate fixed effects of typed polymorphisms simultaneously with remaining linked and unlinked genetic variance. The ADH2*1-2 genotypes metabolize ethanol faster and attain a lower peak blood alcohol concentration than the more common ADH2*1-1 genotypes, although less than 3% of the variance is accounted for. There is no effect of ADH3 genotype. However, sib-pair linkage analysis suggests that there is a linked polymorphism which has a much greater effect on alcohol metabolism that those typed here.

  9. Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    O'Maille, Grace; Go, Eden P.; Hoang, Linh; Want, Elizabeth J.; Smith, Colin; O'Maille, Paul; NordstrÖm, Anders; Morita, Hirotoshi; Qin, Chuan; Uritboonthai, Wilasinee; et al

    2008-01-01

    Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.

  10. Predicting the impacts of climate change on animal distributions: the importance of local adaptation and species' traits

    SciTech Connect (OSTI)

    HELLMANN, J. J.; LOBO, N. F.

    2011-12-20

    The geographic range limits of many species are strongly affected by climate and are expected to change under global warming. For species that are able to track changing climate over broad geographic areas, we expect to see shifts in species distributions toward the poles and away from the equator. A number of ecological and evolutionary factors, however, could restrict this shifting or redistribution under climate change. These factors include restricted habitat availability, restricted capacity for or barriers to movement, or reduced abundance of colonists due the perturbation effect of climate change. This research project examined the last of these constraints - that climate change could perturb local conditions to which populations are adapted, reducing the likelihood that a species will shift its distribution by diminishing the number of potential colonists. In the most extreme cases, species ranges could collapse over a broad geographic area with no poleward migration and an increased risk of species extinction. Changes in individual species ranges are the processes that drive larger phenomena such as changes in land cover, ecosystem type, and even changes in carbon cycling. For example, consider the poleward range shift and population outbreaks of the mountain pine beetle that has decimated millions of acres of Douglas fir trees in the western US and Canada. Standing dead trees cause forest fires and release vast quantities of carbon to the atmosphere. The beetle likely shifted its range because it is not locally adapted across its range, and it appears to be limited by winter low temperatures that have steadily increased in the last decades. To understand range and abundance changes like the pine beetle, we must reveal the extent of adaptive variation across species ranges - and the physiological basis of that adaptation - to know if other species will change as readily as the pine beetle. Ecologists tend to assume that range shifts are the dominant response of species to climate change, but our experiments suggest that other processes may act in some species that reduce the likelihood of geographic range change. In the first part of our DOE grant (ending 2008) we argued that the process of local adaptation of populations within a species range, followed by climatic changes that occur too quickly for adaptive evolution, is an underappreciated mechanism by which climate change could affect biodiversity. When this process acts, species ranges may not shift readily toward the poles, slowing the rate of species and biome change. To test this claim, we performed an experiment comparing core and peripheral populations in a series of field observations, translocation experiments, and genetic analyses. The papers in Appendix A were generated from 2005-2008 funding. In the second part of the DOE grant (ending 2011) we studied which traits promote population differentiation and local adaptation by building genomic resources for our study species and using these resources to reveal differences in gene expression in peripheral and core populations. The papers in Appendix B were generated from 2008-2011 funding. This work was pursued with two butterfly species that have contrasting life history traits (body size and resource specialization) and occupy a common ecosystem and a latitudinal range. These species enabled us to test the following hypotheses using a single phylogenetic group.

  11. Advances in Quantitative Ion Character-Activity Relationships (QICARs): Using Metal-Ligand Binding Characteristics to Predict

    E-Print Network [OSTI]

    Fabrizio, Mary C.

    Abstract Environmental toxicologists readily adopted QSARs from pharmacology to predict organic contaminant explored and underutilized. Quantitative Ion Character-Activity Relationships (QI- CARs) have recently been

  12. Complete Quantitative online Analysis of Methanol Electrooxidation Prod-ucts via Electron Impact and Electrospray Ionization Mass Spectrometry

    E-Print Network [OSTI]

    Pfeifer, Holger

    1 Complete Quantitative online Analysis of Methanol Electrooxidation Prod- ucts via Electron Impact for application in energy re- lated electrocatalysis is demonstrated using continuous methanol oxidation over

  13. A quantitative comparison of rates of phagocytosis and digestion of apoptotic cells by macrophages from normal

    E-Print Network [OSTI]

    Keshet, Leah

    A quantitative comparison of rates of phagocytosis and digestion of apoptotic cells by macrophages head: Phagocytosis and digestion by NOD mice macrophages Corresponding author: Leah Edelstein, thus quantifying kinetics of uptake and digestion of apoptotic cells in both mouse strains (Mar´ee et

  14. A new method for quantitative analysis of multiple scelerosis using MR images

    E-Print Network [OSTI]

    analysis of multiple sclerosis (MS) was presented. An automatic self-adaptive image segmentation algorithm Analysis, Multipl Sclerosis, Brain Atrophy 1. INTRODUCTION Multiple sclerosis (MS) is the most commonA new method for quantitative analysis of multiple scelerosis using MR images Dongqing Chen*a , Wei

  15. Intraoperative Brain Shift and Deformation: A Quantitative Analysis of Cortical Displacement in 28 Cases

    E-Print Network [OSTI]

    Frey, Pascal

    Intraoperative Brain Shift and Deformation: A Quantitative Analysis of Cortical Displacement in 28 of surgery, the nature of the cranial opening, the region of the brain involved, the duration of surgery resulting from misregistration during the course of surgery is warranted. Key words: brain shift, computer

  16. What is Actuarial Science? Actuarial science is the quantitative analysis of risk.

    E-Print Network [OSTI]

    Saldin, Dilano

    What is Actuarial Science? Actuarial science is the quantitative analysis of risk. In addition and computer programming. What Do Actuaries Do? Actuaries help individuals, businesses and society manage risk: · What price best balances an insurance company's risk tolerance with various risk parameters such as age

  17. Reduced xenon diffusion for quantitative lung studythe role of SF6

    E-Print Network [OSTI]

    Walsworth, Ronald L.

    Reduced xenon diffusion for quantitative lung studyĐthe role of SF6 Ross W. Mair,1 * Dominik in most NMR experiments. In restricted environments, such as the lung, this rapid gas diffusion can lead the development of lung ventilation MRI using laser-polarized noble gases (129 Xe and 3 He),1,2 attention has been

  18. Sources of Technical Variability in Quantitative LC-MS Proteomics: Human Brain Tissue Sample Analysis.

    SciTech Connect (OSTI)

    Piehowski, Paul D.; Petyuk, Vladislav A.; Orton, Daniel J.; Xie, Fang; Moore, Ronald J.; Ramirez Restrepo, Manuel; Engel, Anzhelika; Lieberman, Andrew P.; Albin, Roger L.; Camp, David G.; Smith, Richard D.; Myers, Amanda J.

    2013-05-03

    To design a robust quantitative proteomics study, an understanding of both the inherent heterogeneity of the biological samples being studied as well as the technical variability of the proteomics methods and platform is needed. Additionally, accurately identifying the technical steps associated with the largest variability would provide valuable information for the improvement and design of future processing pipelines. We present an experimental strategy that allows for a detailed examination of the variability of the quantitative LC-MS proteomics measurements. By replicating analyses at different stages of processing, various technical components can be estimated and their individual contribution to technical variability can be dissected. This design can be easily adapted to other quantitative proteomics pipelines. Herein, we applied this methodology to our label-free workflow for the processing of human brain tissue. For this application, the pipeline was divided into four critical components: Tissue dissection and homogenization (extraction), protein denaturation followed by trypsin digestion and SPE clean-up (digestion), short-term run-to-run instrumental response fluctuation (instrumental variance), and long-term drift of the quantitative response of the LC-MS/MS platform over the 2 week period of continuous analysis (instrumental stability). From this analysis, we found the following contributions to variability: extraction (72%) >> instrumental variance (16%) > instrumental stability (8.4%) > digestion (3.1%). Furthermore, the stability of the platform and its’ suitability for discovery proteomics studies is demonstrated.

  19. Quantitative Depth Profiling of Biporous Nickel Electrodes by Frequency-Domain Laser-Induced

    E-Print Network [OSTI]

    Mandelis, Andreas

    for the long-term stability and high efficiency of electrochemical energy conversion in fuel cells manufactured for use in electrochemical energy conversion de- vices, such as fuel cells. Index Headings: Laser-induced photoacoustic spectroscopy; Variable electrode porosities; Frequency response; Quantitative analysis; Fuel cells

  20. Timedomain MRSI quantitation combining multivoxel processing, FIR based solvent suppression and prior knowledge

    E-Print Network [OSTI]

    Time­domain MRSI quantitation combining multivoxel processing, FIR based solvent suppression and prior knowledge P. Pels#, L. Vanhamme#, S. Van Hu#el#, and P. Van Hecke + # Katholieke Universiteit processing using a FIR filter for solvent suppression. The advantage of this multivoxel approach

  1. Three-Dimensional Quantitative Microwave Imaging of Realistic Numerical Breast Phantoms Using Huber Regularization

    E-Print Network [OSTI]

    Pizurica, Aleksandra

    Three-Dimensional Quantitative Microwave Imaging of Realistic Numerical Breast Phantoms Using Huber detection with microwaves is based on the difference in dielectric properties between normal and malignant microwave scattering is a nonlinear, ill-posed inverse problem. We proposed to use the Huber regularization

  2. QUANTITATIVE DAMAGE ASSESSMENT OF HYBRID COMPOSITE WIND TURBINE BLADES BY ENERGY BASED ACOUSTIC EMISSION SOURCE

    E-Print Network [OSTI]

    Boyer, Edmond

    QUANTITATIVE DAMAGE ASSESSMENT OF HYBRID COMPOSITE WIND TURBINE BLADES BY ENERGY BASED ACOUSTIC in the wind turbine blade. It was tried to apply a new source location method, which has a developed algorithm assessment, source location, wind turbine blade, hybrid composites INTRODUCTION Structural health management

  3. Quantitative predictions of tokamak energy confinement from first-principles simulations with kinetic effects*

    E-Print Network [OSTI]

    Hammett, Greg

    Quantitative predictions of tokamak energy confinement from first-principles simulations Jersey 08543 (Received 14 November 1994; accepted 2 March 1995) A first-principles model of anomalous data from the Tokamak Fusion Test Reactor (TFTR) [Fusion Technol. 21, 1324 (1992)]. This model is based

  4. Quantitative Predictions of Tokamak Energy Confinement from FirstPrinciples Simulations with Kinetic Effects

    E-Print Network [OSTI]

    Hammett, Greg

    Quantitative Predictions of Tokamak Energy Confinement from First­Principles Simulations 451, Princeton, NJ, 08543 Abstract A first­principles model of anomalous thermal transport based Fusion Test Reactor (TFTR) [Fusion Technol. 21, 1324 (1992)]. This model is based on nonlinear gyrofluid

  5. Weaving Versus Blending: a quantitative assessment of the information carrying capacities of two alternative methods for

    E-Print Network [OSTI]

    Healey, Christopher G.

    as the number of components increases from 4 to 6. We found no significant advantages, in either color blendingWeaving Versus Blending: a quantitative assessment of the information carrying capacities of two: color blending and color weaving. We begin with a baseline experiment in which we assess participants

  6. Substrate Hydroxylation in Methane Monooxygenase: Quantitative Modeling via Mixed Quantum Mechanics/

    E-Print Network [OSTI]

    Gherman, Benjamin F.

    Substrate Hydroxylation in Methane Monooxygenase: Quantitative Modeling via Mixed Quantum Mechanics with mixed quantum mechanics/molecular mechanics (QM/MM) methods, the hydroxylation of methane. With the current results, recent kinetic data for CH3X (X ) H, CH3, OH, CN, NO2) substrate hydroxylation in MMOH

  7. A quantitative method to evaluate microbial electrolysis cell effectiveness for energy recovery

    E-Print Network [OSTI]

    A quantitative method to evaluate microbial electrolysis cell effectiveness for energy recovery Wastewater treatment Energy consumption Coulombic efficiency a b s t r a c t Microbial electrolysis cells 2013 Accepted 31 July 2013 Available online 27 August 2013 Keywords: Microbial electrolysis cell

  8. Quantitative estimates of magnetic field reconnection properties from electric and magnetic field measurements

    E-Print Network [OSTI]

    California at Berkeley, University of

    Quantitative estimates of magnetic field reconnection properties from electric and magnetic field there are positive electric field components tangential to the magnetopause and a magnetic field component normal to it. Because these three components are the smallest of the six electric and magnetic fields

  9. Quantitative Analysis of Transmission Power Control in Wireless Ad-hoc Networks

    E-Print Network [OSTI]

    Sivakumar, Raghupathy

    -hoc network is determined by the transmission power of the stations. Be- cause different topologies have transmis- sion power is determined by the network load, the number of stations, and the network size1 Quantitative Analysis of Transmission Power Control in Wireless Ad-hoc Networks Seung-Jong Park

  10. Energy-Efficiency Comparison of Mobile Platforms and Applications: A Quantitative Approach

    E-Print Network [OSTI]

    Shi, Weisong

    Energy-Efficiency Comparison of Mobile Platforms and Applications: A Quantitative Approach Grace comparing mobile platforms and apps for energy efficiency. In addition, based on case studies that focus on energy efficiency comparison of different app cat- egories on the most popular platforms, the paper

  11. Towards quantitative three-dimensional characterisation of InAs quantum S. Kadkhodazadeh1

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    Dots, Electron Tomography, Atom Probe Tomography InAs quantum dots (QDs) grown on InP or InTowards quantitative three-dimensional characterisation of InAs quantum dots S. Kadkhodazadeh1 , E for Microstructure Research, Forschungszentrum Jülich, D-52425 Jülich, Germany shka@cen.dtu.dk Keywords: InAs Quantum

  12. QUANTITATIVE ESTIMATES ON THE HYDROGEN GROUND STATE ENERGY IN NON-RELATIVISTIC QED

    E-Print Network [OSTI]

    QUANTITATIVE ESTIMATES ON THE HYDROGEN GROUND STATE ENERGY IN NON-RELATIVISTIC QED J.-M. BARBAROUX for the hydrogen ground state energy in the Pauli-Fierz model up to the order O(5 log -1), where denotes). As a consequence, we prove that the ground state energy is not a real analytic function of , and verify

  13. Quantitative Analysis of the Resolved X-ray Emission Line Profiles of O Stars

    E-Print Network [OSTI]

    Cohen, David

    Department of Physics & Astronomy Swarthmore College June 21, 2007 - Potsdam - Clumping in Hot-Star Winds with Maurice Leutenegger (Columbia University) #12;1. The wind profile model and quantitative model fitting 2. Smooth wind models: constraints on mass-loss rates 3. Models with porosity: the * - h trade-off 4. A note

  14. Nuclear matrix association of the human b-globin locus utilizing a novel approach to quantitative

    E-Print Network [OSTI]

    Draghici, Sorin

    Nuclear matrix association of the human b-globin locus utilizing a novel approach to quantitative- hypersensitive sites (HS) ~15 kb upstream of the e-globin gene. Nuclear matrix-associated regions (MARs) organize chromatin into functional domains and at least one of the HS appears bound to the nuclear matrix. We have

  15. Big Data is Not About the Data! Institute for Quantitative Social Science

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Big Data is Not About the Data! Gary King1 Institute for Quantitative Social Science Harvard Value in Big Data: the Analytics 2/10 #12;The Value in Big Data: the Analytics · Data: 2/10 #12;The Value in Big Data: the Analytics · Data: · easy to come by; often a free byproduct of IT improvements 2

  16. ANSI/ASHRAE/IES Standard 90.1-2013 Preliminary Determination: Quantitative Analysis

    SciTech Connect (OSTI)

    Halverson, Mark A.; Rosenberg, Michael I.; Wang, Weimin; Zhang, Jian; Mendon, Vrushali V.; Athalye, Rahul A.; Xie, YuLong; Hart, Reid; Goel, Supriya

    2014-03-01

    This report provides a preliminary quantitative analysis to assess whether buildings constructed according to the requirements of ANSI/ASHRAE/IES Standard 90.1-2013 would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IES Standard 90.1-2010.

  17. Identification and Quantitation of Urea Precipitates in Flexible Polyurethane Foam Formulations by X-ray Spectromicroscopy

    E-Print Network [OSTI]

    with isocyanates to form carbamic acid, which decomposes to an amine and CO2. Rapid reaction of the primary. The heat evolved due to the exothermic nature of the reactions along with the CO2 produced helps to blowIdentification and Quantitation of Urea Precipitates in Flexible Polyurethane Foam Formulations

  18. A quantitative version of Kirk's fixed point theorem for asymptotic contractions

    E-Print Network [OSTI]

    Gerhardy, Philipp

    A quantitative version of Kirk's fixed point theorem for asymptotic contractions Philipp Gerhardy Abstract In [J.Math.Anal.App.277(2003) 645-650], W.A.Kirk introduced the notion of asymp- totic Introduction In [3], W.A. Kirk proved a fixed-point theorem for so-called asymptotic con- tractions on complete

  19. A quantitative version of Kirk's fixed point theorem for asymptotic contractions

    E-Print Network [OSTI]

    Gerhardy, Philipp

    A quantitative version of Kirk's fixed point theorem for asymptotic contractions Philipp Gerhardy Abstract In [J.Math.Anal.App.277(2003) 645­650], W.A.Kirk introduced the notion of asymp­ totic Introduction In [3], W.A. Kirk proved a fixed­point theorem for so­called asymptotic con­ tractions on complete

  20. MSIS 5303 Quantitative Methods in Business (Management Science Methods or Prescriptive Business Analytics)

    E-Print Network [OSTI]

    MSIS 5303 ­ Quantitative Methods in Business (Management Science Methods or Prescriptive Business L. Wilson, Professor of Management Science and Information Systems Contact Information: Office: 408 BUS Email: rick.wilson@okstate.edu Phone: (405) 744-5084 Course Site: Desire2Learn (Online Classroom

  1. A Simulink Hybrid Heart Model for Quantitative Verification of Cardiac Pacemakers

    E-Print Network [OSTI]

    Oxford, University of

    A Simulink Hybrid Heart Model for Quantitative Verification of Cardiac Pacemakers Taolue Chen Mereacre Department of Computer Science University of Oxford, UK ABSTRACT We develop a novel hybrid heart. The heart model is formulated at the level of cardiac cells, can be adapted to patient data, and incorpo

  2. Quantitative linear and nonlinear resonance inspection techniques and analysis for material characterization

    E-Print Network [OSTI]

    and in concrete and cement based materials.2 In the context of license renewal in the field of nuclear energy moduli taking into account the 3D nature of the samples. In the context of license renewal in the field of nuclear energy, this study aims at providing some quantitative information related to the degree of micro

  3. Ultrasonic backscatter coefficient quantitative estimates from Chinese hamster ovary cell pellet biophantoms

    E-Print Network [OSTI]

    Illinois at Urbana-Champaign, University of

    Ultrasonic backscatter coefficient quantitative estimates from Chinese hamster ovary cell pellet August 2010; accepted 6 August 2010 A cell pellet biophantom technique is introduced, and applied verification purposes. BSC estimates from CHO cell pellet biophantoms of known number density were performed

  4. A Quantitative Analysis of Disk Drive Power Management in Portable Computers

    E-Print Network [OSTI]

    Anderson, Tom

    significantly from the 3­5 minutes in current practice by industry. We will show in this paper the effectA Quantitative Analysis of Disk Drive Power Management in Portable Computers Kester Li, Roger Kumpf Abstract With the advent and subsequent popularity of portable computers, power management of system

  5. Selenium and Lung Cancer: A Quantitative Analysis of Heterogeneity in the Current

    E-Print Network [OSTI]

    California at Berkeley, University of

    Selenium and Lung Cancer: A Quantitative Analysis of Heterogeneity in the Current Epidemiological on sele- nium and lung cancer and identify sources of heterogeneity among studies. When all studies were.30). Overall, these results suggest that selenium may have some protective effect against lung cancer

  6. Cellular Patterns in the Inner Retina of Adult Zebrafish: Quantitative Analyses

    E-Print Network [OSTI]

    Carney, Laurel H.

    Cellular Patterns in the Inner Retina of Adult Zebrafish: Quantitative Analyses and a Computational 13244 ABSTRACT The mechanisms that control cellular pattern formation in the growing vertebrate central nervous system are poorly understood. In an effort to reveal mechanistic rules of cellular pattern

  7. Climate change and the socioeconomics of global food production: A quantitative analysis

    E-Print Network [OSTI]

    Rambaut, Andrew

    1 Climate change and the socioeconomics of global food production: A quantitative analysis of how, Andrew J. Dougill and Piers M. Forster August 2010 Centre for Climate Change Economics and Policy Working Paper No. 29 #12;2 The Centre for Climate Change Economics and Policy (CCCEP) was established

  8. Dimeric Drug Polymeric Nanoparticles with Exceptionally High Drug Loading and Quantitative Loading Efficiency

    E-Print Network [OSTI]

    Cheng, Jianjun

    Dimeric Drug Polymeric Nanoparticles with Exceptionally High Drug Loading and Quantitative LoadingChampaign, Urbana, Illinois 61801, United States Key Laboratory of Smart Drug Delivery, Ministry of Education-molecule drugs in hydrophobic polymers or amphiphilic copolymers has been extensively used for preparing

  9. Quantitative Comparison of Measured Plasma Sheet Electron Energy Flux and Remotely Sensed Auroral Electron Energy Flux

    E-Print Network [OSTI]

    Fillingim, Matthew

    Electron Energy Flux M. O. Fillingim1, (matt@ess.washington.edu), G. K. Parks2, D. Chua1, G. A. Germany3, R intensity ~ precipitating electron energy flux Peak energy flux "near" WIND fQuantitative Comparison of Measured Plasma Sheet Electron Energy Flux and Remotely Sensed Auroral

  10. between the nucleus and cytoplasm occurs through large macromolecular structures, the nuclear pores. Quantitative

    E-Print Network [OSTI]

    Forbes, Douglass

    363 between the nucleus and cytoplasm occurs through large macromolecular structures, the nuclear pores. Quantitative scanning transmission electron microscopy has estimated the mass of a nuclear pore noteworthy in that they saw: 1) the purification of both the yeast and vertebrate nuclear pores, 2

  11. Quantitative Methods for Strategic and Investment Planning in the Oil-Refining

    E-Print Network [OSTI]

    Grossmann, Ignacio E.

    Quantitative Methods for Strategic and Investment Planning in the Oil-Refining Brenno C(ng ­ March 2013 What, Where, When to Invest? Only op*mize streams transfers (fuel;Current Investment Planning Methodology in PETROBRAS Strategy - Increase the supply

  12. Challenges in the Modeling and Quantitative Analysis of Safety-Critical Automotive Systems!

    E-Print Network [OSTI]

    Leue, Stefan

    Challenges in the Modeling and Quantitative Analysis of Safety-Critical Automotive Systems! #12;Joint work with! 2! Matthias Kuntz and Bernd Reh TRW Automotive Stefan Leue Univeristy of Konstanz #12;Outline! ! Safety-Critical Automotive Systems! Safety Analysis Methods in the Automotive Domain

  13. Quantitative Model of Price Diffusion and Market Friction Based on Trading as a Mechanistic Random Process

    E-Print Network [OSTI]

    Quantitative Model of Price Diffusion and Market Friction Based on Trading as a Mechanistic Random 2002; published 13 March 2003) We model trading and price formation in a market under the assumption for the most basic properties of markets, such as the diffusion rate of prices (which is the standard measure

  14. December 17, 2008 Robert Almgren / Encyclopedia of Quantitative Finance Execution Costs 1 Execution Costs

    E-Print Network [OSTI]

    Almgren, Robert F.

    December 17, 2008 Robert Almgren / Encyclopedia of Quantitative Finance Execution Costs 1 Execution Costs Execution costs are the difference in value between an ideal trade and what was actually done. The execution cost of a single completed trade is typically the difference between the final average trade price

  15. OPPORTUNITY: QUANTITATIVE RESEARCH PROFESSIONALS (PhD & Post-Doc levels) Location: Chicago

    E-Print Network [OSTI]

    Plotkin, Joshua B.

    mathematical & statistical techniques to complex data intensive problems Good programming skills (e.g. C/C++) with experience with one or more statistical packages (e.g. R/Matlab) and exposure to one or more scripting excellence. Opportunities are available in a variety of teams including Quantitative Trading, Statistical

  16. Research Paper Quantitative Image Analysis for Evaluating the Coating Thickness and Pore

    E-Print Network [OSTI]

    van Vliet, Lucas J.

    Research Paper Quantitative Image Analysis for Evaluating the Coating Thickness and Pore Distribution in Coated Small Particles F. L. Laksmana,1,3,5 L. J. Van Vliet,2 P. J. A. Hartman Kok,3 H. Vromans method for coating structure based on image analysis, which is particularly promising for the rational

  17. On the rise of turbulent plumes: Quantitative effects of variable entrainment for submarine hydrothermal vents, terrestrial

    E-Print Network [OSTI]

    Kaminski, Edouard

    On the rise of turbulent plumes: Quantitative effects of variable entrainment for submarine on the dynamics. Here we show that commonly used one- dimensional models incorporating a constant entrainment coefficient do not provide satisfying predictions. We propose a new model allowing for variable entrainment

  18. Marine biota response to clastic sediment influx: A quantitative approach Stephen W. Lokier a,

    E-Print Network [OSTI]

    Ali, Mohammed

    Marine biota response to clastic sediment influx: A quantitative approach Stephen W. Lokier a anthropogenically related) to marine carbonate environments over longer time periods. © 2009 Elsevier B.V. All clastic input to the marine environment prohibits the development of carbonate producing organisms

  19. Quantitative evaluation of fracture, healing and re-healing of a reversibly cross-linked polymer

    E-Print Network [OSTI]

    Nemat-Nasser, Sia

    Quantitative evaluation of fracture, healing and re-healing of a reversibly cross-linked polymer Abstract The repeated fracture­healing characteristics of 2MEP4F polymer, a cross-linked polymer based the test, improving our ability to realign the fracture surfaces prior to healing. The specimens have been

  20. Quantitative Study of the Value of Research Libraries: A Foundation for the Evaluation of Digital Libraries

    E-Print Network [OSTI]

    Quantitative Study of the Value of Research Libraries: A Foundation for the Evaluation of Digital Libraries Paul B. Kantor and Tefko Saracevic LIS and Rutgers Distributed Laboratory for Digital Libraries, tefko}@diglib.rutgers.edu Abstract In anticipation of the explosive growth of digital libraries

  1. Quantitative Tools for Dissection of Hydrogen-Producing Metabolic Networks-Final Report

    SciTech Connect (OSTI)

    Rabinowitz, Joshua D.; Dismukes, G.Charles.; Rabitz, Herschel A.; Amador-Noguez, Daniel

    2012-10-19

    During this project we have pioneered the development of integrated experimental-computational technologies for the quantitative dissection of metabolism in hydrogen and biofuel producing microorganisms (i.e. C. acetobutylicum and various cyanobacteria species). The application of these new methodologies resulted in many significant advances in the understanding of the metabolic networks and metabolism of these organisms, and has provided new strategies to enhance their hydrogen or biofuel producing capabilities. As an example, using mass spectrometry, isotope tracers, and quantitative flux-modeling we mapped the metabolic network structure in C. acetobutylicum. This resulted in a comprehensive and quantitative understanding of central carbon metabolism that could not have been obtained using genomic data alone. We discovered that biofuel production in this bacterium, which only occurs during stationary phase, requires a global remodeling of central metabolism (involving large changes in metabolite concentrations and fluxes) that has the effect of redirecting resources (carbon and reducing power) from biomass production into solvent production. This new holistic, quantitative understanding of metabolism is now being used as the basis for metabolic engineering strategies to improve solvent production in this bacterium. In another example, making use of newly developed technologies for monitoring hydrogen and NAD(P)H levels in vivo, we dissected the metabolic pathways for photobiological hydrogen production by cyanobacteria Cyanothece sp. This investigation led to the identification of multiple targets for improving hydrogen production. Importantly, the quantitative tools and approaches that we have developed are broadly applicable and we are now using them to investigate other important biofuel producers, such as cellulolytic bacteria.

  2. Analysis on the Go: Quantitation of Drugs of Abuse in Dried Urine with Digital Microfluidics and Miniature Mass Spectrometry

    E-Print Network [OSTI]

    Zandstra, Peter W.

    Analysis on the Go: Quantitation of Drugs of Abuse in Dried Urine with Digital Microfluidics the development of a method coupling microfluidics and a miniature mass spectrometer, applied to quantitation of drugs of abuse in urine. A custom digital microfluidic system was designed to deliver droplets

  3. Quantitative measurement by artificial vision of small bubbles in flowing mercury

    SciTech Connect (OSTI)

    Paquit, Vincent C [ORNL; Wendel, Mark W [ORNL; Felde, David K [ORNL; Riemer, Bernie [ORNL

    2011-01-01

    At the Spallation Neutron Source (SNS), an accelerator-based neutron source located at the Oak Ridge National Laboratory (Tennessee, USA), the production of neutrons is obtained by accelerating protons against a mercury target. This self-cooling target, however, suffers rapid heat deposition by the beam pulse leading to large pressure changes and thus to cavitations that may be damaging to the container. In order to locally compensate for pressure increases, a small-bubble population is added to the mercury flow using gas bubblers. The geometry of the bubblers being unknown, we are testing several bubblers configurations and are using machine vision techniques to characterize their efficiency by quantitative measurement of the created bubble population. In this paper we thoroughly detail the experimental setup and the image processing techniques used to quantitatively assess the bubble population. To support this approach we are comparing our preliminary results for different bubblers and operating modes, and discuss potential improvements.

  4. Reliability of Quantitative Ultrasonic Assessment of Normal-Tissue Toxicity in Breast Cancer Radiotherapy

    SciTech Connect (OSTI)

    Yoshida, Emi J.; Chen Hao; Torres, Mylin; Andic, Fundagul; Liu Haoyang; Chen Zhengjia; Sun, Xiaoyan; Curran, Walter J.; Liu Tian

    2012-02-01

    Purpose: We have recently reported that ultrasound imaging, together with ultrasound tissue characterization (UTC), can provide quantitative assessment of radiation-induced normal-tissue toxicity. This study's purpose is to evaluate the reliability of our quantitative ultrasound technology in assessing acute and late normal-tissue toxicity in breast cancer radiotherapy. Method and Materials: Our ultrasound technique analyzes radiofrequency echo signals and provides quantitative measures of dermal, hypodermal, and glandular tissue toxicities. To facilitate easy clinical implementation, we further refined this technique by developing a semiautomatic ultrasound-based toxicity assessment tool (UBTAT). Seventy-two ultrasound studies of 26 patients (720 images) were analyzed. Images of 8 patients were evaluated for acute toxicity (<6 months postradiotherapy) and those of 18 patients were evaluated for late toxicity ({>=}6 months postradiotherapy). All patients were treated according to a standard radiotherapy protocol. To assess intraobserver reliability, one observer analyzed 720 images in UBTAT and then repeated the analysis 3 months later. To assess interobserver reliability, three observers (two radiation oncologists and one ultrasound expert) each analyzed 720 images in UBTAT. An intraclass correlation coefficient (ICC) was used to evaluate intra- and interobserver reliability. Ultrasound assessment and clinical evaluation were also compared. Results: Intraobserver ICC was 0.89 for dermal toxicity, 0.74 for hypodermal toxicity, and 0.96 for glandular tissue toxicity. Interobserver ICC was 0.78 for dermal toxicity, 0.74 for hypodermal toxicity, and 0.94 for glandular tissue toxicity. Statistical analysis found significant changes in dermal (p < 0.0001), hypodermal (p = 0.0027), and glandular tissue (p < 0.0001) assessments in the acute toxicity group. Ultrasound measurements correlated with clinical Radiation Therapy Oncology Group (RTOG) toxicity scores of patients in the late toxicity group. Patients with RTOG Grade 1 or 2 had greater ultrasound-assessed toxicity percentage changes than patients with RTOG Grade 0. Conclusion: Early and late radiation-induced effects on normal tissue can be reliably assessed using quantitative ultrasound.

  5. Low-frequency quantitative ultrasound imaging of cell death in vivo

    SciTech Connect (OSTI)

    Sadeghi-Naini, Ali; Falou, Omar; Czarnota, Gregory J.; Department of Radiation Oncology, Odette Cancer Centre, Sunnybrook Health Sciences Centre, Toronto, Ontario M4N 3M5; Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, Ontario M4N 3M5; Department of Radiation Oncology, Faculty of Medicine, University of Toronto, Toronto, Ontario M4N 3M5 ; Papanicolau, Naum; Tadayyon, Hadi; Lee, Justin; Zubovits, Judit; Sadeghian, Alireza; Karshafian, Raffi; Al-Mahrouki, Azza; Giles, Anoja; Kolios, Michael C.

    2013-08-15

    Purpose: Currently, no clinical imaging modality is used routinely to assess tumor response to cancer therapies within hours to days of the delivery of treatment. Here, the authors demonstrate the efficacy of ultrasound at a clinically relevant frequency to quantitatively detect changes in tumors in response to cancer therapies using preclinical mouse models.Methods: Conventional low-frequency and corresponding high-frequency ultrasound (ranging from 4 to 28 MHz) were used along with quantitative spectroscopic and signal envelope statistical analyses on data obtained from xenograft tumors treated with chemotherapy, x-ray radiation, as well as a novel vascular targeting microbubble therapy.Results: Ultrasound-based spectroscopic biomarkers indicated significant changes in cell-death associated parameters in responsive tumors. Specifically changes in the midband fit, spectral slope, and 0-MHz intercept biomarkers were investigated for different types of treatment and demonstrated cell-death related changes. The midband fit and 0-MHz intercept biomarker derived from low-frequency data demonstrated increases ranging approximately from 0 to 6 dBr and 0 to 8 dBr, respectively, depending on treatments administrated. These data paralleled results observed for high-frequency ultrasound data. Statistical analysis of ultrasound signal envelope was performed as an alternative method to obtain histogram-based biomarkers and provided confirmatory results. Histological analysis of tumor specimens indicated up to 61% cell death present in the tumors depending on treatments administered, consistent with quantitative ultrasound findings indicating cell death. Ultrasound-based spectroscopic biomarkers demonstrated a good correlation with histological morphological findings indicative of cell death (r{sup 2}= 0.71, 0.82; p < 0.001).Conclusions: In summary, the results provide preclinical evidence, for the first time, that quantitative ultrasound used at a clinically relevant frequency, in addition to high-frequency ultrasound, can detect tissue changes associated with cell death in vivo in response to cancer treatments.

  6. Quantitative solubility-structure relationships for some meta- and para-substituted benzoic acids 

    E-Print Network [OSTI]

    Pawlowski, John Norman

    1963-01-01

    OVANTITATIVE SOLVBILITV-STRVCTVRE RELATIONSHIPR FOR SOME META- AND FARAMUBSTITDTED BENZOIC ACIDS A Thesis by JOHN NORMAN PAWLOSKI Submitted to the Graduate School of the Agricultural and Mechanical College of Texas in partial fulfillment... of the re- quirements;for the degree of MASTER OF SCIENCE Augus t 1963 Ma)or Sub)ect: Chemistry QUANTITATIVE SOLUBILITY-STRUCTURE RELATIONSHIPS POR SOME META- AND PARA-SUBSTITUTED BENZGIC ACIDS A Thesis JOHN NORMAN PAWLOSKI Approved as to style...

  7. Apparatus and method for quantitative assay of generic transuranic wastes from nuclear reactors

    DOE Patents [OSTI]

    Caldwell, J.T.; Kunz, W.E.; Atencio, J.D.

    1982-03-31

    A combination of passive and active neutron measurements which yields quantitative information about the isotopic composition of transuranic wastes from nuclear power or weapons material manufacture reactors is described. From the measurement of prompt and delayed neutron emission and the incidence of two coincidentally emitted neutrons from induced fission of fissile material in the sample, one can quantify /sup 233/U, /sup 235/U and /sup 239/Pu isotopes in waste samples. Passive coincidence counting, including neutron multiplicity measurement and determination of the overall passive neutron flux additionally enables the separate quantitative evaluation of spontaneous fission isotopes such as /sup 240/Pu, /sup 244/Cm and /sup 252/Cf, and the spontaneous alpha particle emitter /sup 241/Am. These seven isotopes are the most important constituents of wastes from nuclear power reactors and once the mass of each isotope present is determined by the apparatus and method of the instant invention, the overall alpha particle activity can be determined to better than 1 nCi/g from known radioactivity data. Therefore, in addition to the quantitative analysis of the waste sample useful for later reclamation purposes, the alpha particle activity can be determined to decide whether permanent low-level burial is appropriate for the waste sample.

  8. Apparatus and method for quantitative assay of generic transuranic wastes from nuclear reactors

    DOE Patents [OSTI]

    Caldwell, John T. (Los Alamos, NM); Kunz, Walter E. (Santa Fe, NM); Atencio, James D. (Los Alamos, NM)

    1984-01-01

    A combination of passive and active neutron measurements which yields quantitative information about the isotopic composition of transuranic wastes from nuclear power or weapons material manufacture reactors is described. From the measurement of prompt and delayed neutron emission and the incidence of two coincidentally emitted neutrons from induced fission of fissile material in the sample, one can quantify .sup.233 U, .sup.235 U and .sup.239 Pu isotopes in waste samples. Passive coincidence counting, including neutron multiplicity measurement and determination of the overall passive neutron flux additionally enables the separate quantitative evaluation of spontaneous fission isotopes such as .sup.240 Pu, .sup.244 Cm and .sup.252 Cf, and the spontaneous alpha particle emitter .sup.241 Am. These seven isotopes are the most important constituents of wastes from nuclear power reactors and once the mass of each isotope present is determined by the apparatus and method of the instant invention, the overall alpha particle activity can be determined to better than 1 nCi/g from known radioactivity data. Therefore, in addition to the quantitative analysis of the waste sample useful for later reclamation purposes, the alpha particle activity can be determined to decide whether "permanent" low-level burial is appropriate for the waste sample.

  9. Elevated carbon dioxide is predicted to promote coexistence among competing species in a trait-based model

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ali, Ashehad A.; Medlyn, Belinda E.; Aubier, Thomas G.; Crous, Kristine Y.; Reich, Peter B.

    2015-10-06

    Differential species responses to atmospheric CO2 concentration (Ca) could lead to quantitative changes in competition among species and community composition, with flow-on effects for ecosystem function. However, there has been little theoretical analysis of how elevated Ca (eCa) will affect plant competition, or how composition of plant communities might change. Such theoretical analysis is needed for developing testable hypotheses to frame experimental research. Here, we investigated theoretically how plant competition might change under eCa by implementing two alternative competition theories, resource use theory and resource capture theory, in a plant carbon and nitrogen cycling model. The model makes several novelmore »predictions for the impact of eCa on plant community composition. Using resource use theory, the model predicts that eCa is unlikely to change species dominance in competition, but is likely to increase coexistence among species. Using resource capture theory, the model predicts that eCa may increase community evenness. Collectively, both theories suggest that eCa will favor coexistence and hence that species diversity should increase with eCa. Our theoretical analysis leads to a novel hypothesis for the impact of eCa on plant community composition. In this study, the hypothesis has potential to help guide the design and interpretation of eCa experiments.« less

  10. The Application of Traits-Based Assessment Approaches to Estimate the Effects of Hydroelectric Turbine Passage on Fish Populations

    SciTech Connect (OSTI)

    Cada, Glenn F; Schweizer, Peter E

    2012-04-01

    One of the most important environmental issues facing the hydropower industry is the adverse impact of hydroelectric projects on downstream fish passage. Fish that migrate long distances as part of their life cycle include not only important diadromous species (such as salmon, shads, and eels) but also strictly freshwater species. The hydropower reservoirs that downstream-moving fish encounter differ greatly from free-flowing rivers. Many of the environmental changes that occur in a reservoir (altered water temperature and transparency, decreased flow velocities, increased predation) can reduce survival. Upon reaching the dam, downstream-migrating fish may suffer increased mortality as they pass through the turbines, spillways and other bypasses, or turbulent tailraces. Downstream from the dam, insufficient environmental flow releases may slow downstream fish passage rates or decrease survival. There is a need to refine our understanding of the relative importance of causative factors that contribute to turbine passage mortality (e.g., strike, pressure changes, turbulence) so that turbine design efforts can focus on mitigating the most damaging components. Further, present knowledge of the effectiveness of turbine improvements is based on studies of only a few species (mainly salmon and American shad). These data may not be representative of turbine passage effects for the hundreds of other fish species that are susceptible to downstream passage at hydroelectric projects. For example, there are over 900 species of fish in the United States. In Brazil there are an estimated 3,000 freshwater fish species, of which 30% are believed to be migratory (Viana et al. 2011). Worldwide, there are some 14,000 freshwater fish species (Magurran 2009), of which significant numbers are susceptible to hydropower impacts. By comparison, in a compilation of fish entrainment and turbine survival studies from over 100 hydroelectric projects in the United States, Winchell et al. (2000) found useful turbine passage survival data for only 30 species. Tests of advanced hydropower turbines have been limited to seven species - Chinook and coho salmon, rainbow trout, alewife, eel, smallmouth bass, and white sturgeon. We are investigating possible approaches for extending experimental results from the few tested fish species to predict turbine passage survival of other, untested species (Cada and Richmond 2011). In this report, we define the causes of injury and mortality to fish tested in laboratory and field studies, based on fish body shape and size, internal and external morphology, and physiology. We have begun to group the large numbers of unstudied species into a small number of categories, e.g., based on phylogenetic relationships or ecological similarities (guilds), so that subsequent studies of a few representative species (potentially including species-specific Biological Index Testing) would yield useful information about the overall fish community. This initial effort focused on modifying approaches that are used in the environmental toxicology field to estimate the toxicity of substances to untested species. Such techniques as the development of species sensitivity distributions (SSDs) and Interspecies Correlation Estimation (ICE) models rely on a considerable amount of data to establish the species-toxicity relationships that can be extended to other organisms. There are far fewer studies of turbine passage stresses from which to derive the turbine passage equivalent of LC{sub 50} values. Whereas the SSD and ICE approaches are useful analogues to predicting turbine passage injury and mortality, too few data are available to support their application without some form of modification or simplification. In this report we explore the potential application of a newer, related technique, the Traits-Based Assessment (TBA), to the prediction of downstream passage mortality at hydropower projects.

  11. The laminar flow tube reactor as a quantitative tool for nucleation studies: Experimental results and theoretical analysis of homogeneous

    E-Print Network [OSTI]

    Ford, Ian

    The laminar flow tube reactor as a quantitative tool for nucleation studies: Experimental results, United Kingdom Received 24 March 2000; accepted 2 June 2000 A laminar flow tube reactor was designed

  12. Abstracts of papers presented at the LVIII Cold Spring Harbor Symposium on quantitative Biology: DNA and chromosomes

    SciTech Connect (OSTI)

    Not Available

    1993-12-31

    This volume contains the abstracts of oral and poster presentations made at the LVIII Cold Spring Harbor Symposium on Quantitative Biology entitles DNA & Chromosomes. The meeting was held June 2--June 9, 1993 at Cold Spring Harbor, New York.

  13. Benchtop testing of polyethylene passive sampling towards a quantitative analysis of volatile organic compounds (VOCs) in soil vapours

    E-Print Network [OSTI]

    Soo, Yu Xiang Jaren

    2015-01-01

    The feasibility of polyethylene (PE) as a passive sampler for quantitative analysis of volatile organic compounds (VOCs) was analysed in this work by means of a benchtop testing. A benchtop physical model was setup, which ...

  14. High Resolution Quantitative Auto-Radiography to determine microscopic distributions of B-10 in neutron capture therapy

    E-Print Network [OSTI]

    Harris, Thomas C. (Thomas Cameron)

    2006-01-01

    The success of Boron Neutron Capture Therapy (BNCT) is heavily dependent on the microscopic distribution of B-10 in tissue. High Resolution Quantitative Auto-Radiography (HRQAR) is a potentially valuable analytical tool ...

  15. Description & Careers Business Analytics and Information Technology (BAIT) is Rutgers Business School's quantitative and computing major. It

    E-Print Network [OSTI]

    Description & Careers The Field Business Analytics and Information Technology (BAIT) is Rutgers Business School's quantitative and computing major. It covers information technology, data analysis skills needed for the converging fields of information technology and business analytics. Skills taught

  16. From Single-Cell Genetic Architecture to Cell Population Dynamics: Quantitatively Decomposing the Effects of Different Population

    E-Print Network [OSTI]

    Bernard, Samuel

    From Single-Cell Genetic Architecture to Cell Population Dynamics: Quantitatively Decomposing Architecture Nikos V. Mantzaris Department of Chemical and Biomolecular Engineering, Rice University, Houston with positive feedback architecture. The framework consists of three vastly different mathematical formulations

  17. The interaction of THz phonon-polariton waves with microstructures observed using quantitative, phase-sensitive imaging

    E-Print Network [OSTI]

    Werley, Christopher Alan

    We apply newly developed, phase-sensitive imaging to enable sharply focused visualization of terahertz waves in electro-optic media. This approach allows quantitative characterization of THz waves as they interact with ...

  18. A quantitative proteomics study of the additive effect of inflammatory cytokines and injurious compression on cartilage damage

    E-Print Network [OSTI]

    Swaminathan, Krishnakumar

    2011-01-01

    Objectives: 1) To perform a quantitative comparison of proteins released to media on combination with cytokine (IL-1[beta[ or TNF-[alpha]) and Injury as compared to either treatment alone, and to thus identify proteins ...

  19. Improved Protein Arrays for Quantitative Systems Analysis of the Dynamics of Signaling Pathway Interactions

    SciTech Connect (OSTI)

    YANG, CHIN-RANG

    2013-12-11

    Astronauts and workers in nuclear plants who repeatedly exposed to low doses of ionizing radiation (IR, <10 cGy) are likely to incur specific changes in signal transduction and gene expression in various tissues of their body. Remarkable advances in high throughput genomics and proteomics technologies enable researchers to broaden their focus from examining single gene/protein kinetics to better understanding global gene/protein expression profiling and biological pathway analyses, namely Systems Biology. An ultimate goal of systems biology is to develop dynamic mathematical models of interacting biological systems capable of simulating living systems in a computer. This Glue Grant is to complement Dr. Boothman’s existing DOE grant (No. DE-FG02-06ER64186) entitled “The IGF1/IGF-1R-MAPK-Secretory Clusterin (sCLU) Pathway: Mediator of a Low Dose IR-Inducible Bystander Effect” to develop sensitive and quantitative proteomic technology that suitable for low dose radiobiology researches. An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states for systems biology modeling is presented. The signals are amplified by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots and show the good linearity that is impossible for the signals of HRP-amplification. Therefore this improved protein array technology is suitable to detect weak responses of low dose radiation. Software is developed to facilitate the quantitative readout of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.

  20. A x-ray radiography-densitometry technique for the quantitative determination of metal deposit profiles

    SciTech Connect (OSTI)

    Will, F.G.; Iacovangelo, C.D.

    1984-03-01

    The application of x-ray radiography in conjunction with high resolution optical densitometry for the quantitative determination of metal deposit profiles parallel and perpendicular to the substrate surface is described. The principles of the technique and the range of its applicability are discussed. The technique is applied to the study of zinc deposition on highly porous carbon foams from circulating aqueous zinc bromide solutions. The effect of substrate pore size on the zinc distribution is explored. Zinc is found to deposit predominantly on the porous substrate/electrolyte and substrate/current collector interfaces. Smaller pore size favors smoother and more uniform deposits throughout the substrate.

  1. Spatially resolved quantitative mapping of thermomechanical properties and phase transition temperatures using scanning probe microscopy

    DOE Patents [OSTI]

    Jesse, Stephen; Kalinin, Sergei V; Nikiforov, Maxim P

    2013-07-09

    An approach for the thermomechanical characterization of phase transitions in polymeric materials (polyethyleneterephthalate) by band excitation acoustic force microscopy is developed. This methodology allows the independent measurement of resonance frequency, Q factor, and oscillation amplitude of a tip-surface contact area as a function of tip temperature, from which the thermal evolution of tip-surface spring constant and mechanical dissipation can be extracted. A heating protocol maintained a constant tip-surface contact area and constant contact force, thereby allowing for reproducible measurements and quantitative extraction of material properties including temperature dependence of indentation-based elastic and loss moduli.

  2. Quantitative analysis of tin alloy combined with artificial neural network prediction

    SciTech Connect (OSTI)

    Oh, Seong Y.; Yueh, Fang-Yu; Singh, Jagdish P.

    2010-05-01

    Laser-induced breakdown spectroscopy was applied to quantitative analysis of three impurities in Sn alloy. The impurities analysis was based on the internal standard method using the Sn I 333.062-nm line as the reference line to achieve the best reproducible results. Minor-element concentrations (Ag, Cu, Pb) in the alloy were comparatively evaluated by artificial neural networks (ANNs) and calibration curves. ANN was found to effectively predict elemental concentrations with a trend of nonlinear growth due to self-absorption. The limits of detection for Ag, Cu, and Pb in Sn alloy were determined to be 29, 197, and 213 ppm, respectively.

  3. Quantitative analysis of reptation of partially extended DNA in sub-30 nm nanoslits

    E-Print Network [OSTI]

    Jia-Wei Yeh; K. K. Sriram; Alessandro Taloni; Yeng-Long Chen; Chia-Fu Chou

    2015-02-18

    We observed reptation of single DNA molecules in fused silica nanoslits of sub-30 nm height. The reptation behavior and the effect of confinement are quantitatively characterized using orientation correlation and transverse fluctuation analysis. We show tube-like polymer motion arises for a tense polymer under strong quasi-2D confinement and interaction with surface- passivating polyvinylpyrrolidone (PVP) molecules in nanoslits, while etching- induced device surface roughness, chip bonding materials and DNA-intercalated dye-surface interaction, play minor roles. These findings have strong implications for the effect of surface modification in nanofluidic systems with potential applications for single molecule DNA analysis.

  4. Apparatus and method for quantitative determination of materials contained in fluids

    DOE Patents [OSTI]

    Radziemski, L.J.; Cremers, D.A.

    1982-09-07

    Apparatus and method for near real-time in-situ monitoring of particulates and vapors contained in fluids are described. Initial filtration of a known volume of the fluid sample is combined with laser-induced dielectric breakdown spectroscopy of the filter employed to obtain qualitative and quantitative information with high sensitivity. Application of the invention to monitoring of beryllium, beryllium oxide, or other beryllium-alloy dusts is shown. Significant shortening of analysis time is achieved from the usual chemical techniques of analysis.

  5. Comprehensive, Quantitative Risk Assessment of CO{sub 2} Geologic Sequestration

    SciTech Connect (OSTI)

    Lepinski, James

    2013-09-30

    A Quantitative Failure Modes and Effects Analysis (QFMEA) was developed to conduct comprehensive, quantitative risk assessments on CO{sub 2} capture, transportation, and sequestration or use in deep saline aquifers, enhanced oil recovery operations, or enhanced coal bed methane operations. The model identifies and characterizes potential risks; identifies the likely failure modes, causes, effects and methods of detection; lists possible risk prevention and risk mitigation steps; estimates potential damage recovery costs, mitigation costs and costs savings resulting from mitigation; and ranks (prioritizes) risks according to the probability of failure, the severity of failure, the difficulty of early failure detection and the potential for fatalities. The QFMEA model generates the necessary information needed for effective project risk management. Diverse project information can be integrated into a concise, common format that allows comprehensive, quantitative analysis, by a cross-functional team of experts, to determine: What can possibly go wrong? How much will damage recovery cost? How can it be prevented or mitigated? What is the cost savings or benefit of prevention or mitigation? Which risks should be given highest priority for resolution? The QFMEA model can be tailored to specific projects and is applicable to new projects as well as mature projects. The model can be revised and updated as new information comes available. It accepts input from multiple sources, such as literature searches, site characterization, field data, computer simulations, analogues, process influence diagrams, probability density functions, financial analysis models, cost factors, and heuristic best practices manuals, and converts the information into a standardized format in an Excel spreadsheet. Process influence diagrams, geologic models, financial models, cost factors and an insurance schedule were developed to support the QFMEA model. Comprehensive, quantitative risk assessments were conducted on three (3) sites using the QFMEA model: (1) SACROC Northern Platform CO{sub 2}-EOR Site in the Permian Basin, Scurry County, TX, (2) Pump Canyon CO{sub 2}-ECBM Site in the San Juan Basin, San Juan County, NM, and (3) Farnsworth Unit CO{sub 2}-EOR Site in the Anadarko Basin, Ochiltree County, TX. The sites were sufficiently different from each other to test the robustness of the QFMEA model.

  6. Apparatus and method for quantitative determination of materials contained in fluids

    DOE Patents [OSTI]

    Radziemski, Leon J. (Los Alamos, NM); Cremers, David A. (Los Alamos, NM)

    1985-01-01

    Apparatus and method for near real-time in-situ monitoring of particulates and vapors contained in fluids. Initial filtration of a known volume of the fluid sample is combined with laser-induced dielectric breakdown spectroscopy of the filter employed to obtain qualitative and quantitative information with high sensitivity. Application of the invention to monitoring of beryllium, beryllium oxide, or other beryllium-alloy dusts is demonstrated. Significant shortening of analysis time is achieved from those of the usual chemical techniques of analysis.

  7. Quantitative ultrasound characterization of locally advanced breast cancer by estimation of its scatterer properties

    SciTech Connect (OSTI)

    Tadayyon, Hadi; Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5G 2M9 ; Sadeghi-Naini, Ali; Czarnota, Gregory; Department of Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5G 2M9; Department of Radiation Oncology, Odette Cancer Centre, Sunnybrook Health Sciences Centre, Toronto, Ontario M4N 3M5; Department of Radiation Oncology, Faculty of Medicine, University of Toronto, Toronto, Ontario M5T 1P5 ; Wirtzfeld, Lauren; Wright, Frances C.

    2014-01-15

    Purpose: Tumor grading is an important part of breast cancer diagnosis and currently requires biopsy as its standard. Here, the authors investigate quantitative ultrasound parameters in locally advanced breast cancers that can potentially separate tumors from normal breast tissue and differentiate tumor grades. Methods: Ultrasound images and radiofrequency data from 42 locally advanced breast cancer patients were acquired and analyzed. Parameters related to the linear regression of the power spectrum—midband fit, slope, and 0-MHz-intercept—were determined from breast tumors and normal breast tissues. Mean scatterer spacing was estimated from the spectral autocorrelation, and the effective scatterer diameter and effective acoustic concentration were estimated from the Gaussian form factor. Parametric maps of each quantitative ultrasound parameter were constructed from the gated radiofrequency segments in tumor and normal tissue regions of interest. In addition to the mean values of the parametric maps, higher order statistical features, computed from gray-level co-occurrence matrices were also determined and used for characterization. Finally, linear and quadratic discriminant analyses were performed using combinations of quantitative ultrasound parameters to classify breast tissues. Results: Quantitative ultrasound parameters were found to be statistically different between tumor and normal tissue (p < 0.05). The combination of effective acoustic concentration and mean scatterer spacing could separate tumor from normal tissue with 82% accuracy, while the addition of effective scatterer diameter to the combination did not provide significant improvement (83% accuracy). Furthermore, the two advanced parameters, including effective scatterer diameter and mean scatterer spacing, were found to be statistically differentiating among grade I, II, and III tumors (p = 0.014 for scatterer spacing, p = 0.035 for effective scatterer diameter). The separation of the tumor grades further improved when the textural features of the effective scatterer diameter parametric map were combined with the mean value of the map (p = 0.004). Conclusions: Overall, the binary classification results (tumor versus normal tissue) were more promising than tumor grade assessment. Combinations of advanced parameters can further improve the separation of tumors from normal tissue compared to the use of linear regression parameters. While the linear regression parameters were sufficient for characterizing breast tumors and normal breast tissues, advanced parameters and their textural features were required to better characterize tumor subtypes.

  8. Quantitative Reflectance Spectra of Solid Powders as a Function of Particle Size

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Myers, Tanya L.; Brauer, Carolyn S.; Su, Yin-Fong; Blake, Thomas A.; Tonkyn, Russell G.; Ertel, Alyssa B.; Johnson, Timothy J.; Richardson, Robert L.

    2015-05-19

    We have recently developed vetted methods for obtaining quantitative infrared directional-hemispherical reflectance spectra using a commercial integrating sphere. In this paper, the effects of particle size on the spectral properties are analyzed for several samples such as ammonium sulfate, calcium carbonate, and sodium sulfate as well as one organic compound, lactose. We prepared multiple size fractions for each sample and confirmed the mean sizes using optical microscopy. Most species displayed a wide range of spectral behavior depending on the mean particle size. General trends of reflectance vs. particle size are observed such as increased albedo for smaller particles: for mostmore »wavelengths, the reflectivity drops with increased size, sometimes displaying a factor of 4 or more drop in reflectivity along with a loss of spectral contrast. In the longwave infrared, several species with symmetric anions or cations exhibited reststrahlen features whose amplitude was nearly invariant with particle size, at least for intermediate- and large-sized sample fractions; that is, > ~150 microns. Trends of other types of bands (Christiansen minima, transparency features) are also investigated as well as quantitative analysis of the observed relationship between reflectance vs. particle diameter.« less

  9. Surface and grain boundary scattering in nanometric Cu thin films: A quantitative analysis including twin boundaries

    SciTech Connect (OSTI)

    Barmak, Katayun [Department of Applied Physics and Applied Mathematics, Columbia University, New York, New York 10027 and Department of Materials Science and Engineering and Materials Research Science and Engineering Center, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, Pennsylvania 15213 (United States); Darbal, Amith [Department of Materials Science and Engineering and Materials Research Science and Engineering Center, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, Pennsylvania 15213 (United States); Ganesh, Kameswaran J.; Ferreira, Paulo J. [Materials Science and Engineering, The University of Texas at Austin, 1 University Station, Austin, Texas 78712 (United States); Rickman, Jeffrey M. [Department of Materials Science and Engineering and Department of Physics, Lehigh University, Bethlehem, Pennsylvania 18015 (United States); Sun, Tik; Yao, Bo; Warren, Andrew P.; Coffey, Kevin R., E-mail: kb2612@columbia.edu [Department of Materials Science and Engineering, University of Central Florida, 4000 Central Florida Boulevard, Orlando, Florida 32816 (United States)

    2014-11-01

    The relative contributions of various defects to the measured resistivity in nanocrystalline Cu were investigated, including a quantitative account of twin-boundary scattering. It has been difficult to quantitatively assess the impact twin boundary scattering has on the classical size effect of electrical resistivity, due to limitations in characterizing twin boundaries in nanocrystalline Cu. In this study, crystal orientation maps of nanocrystalline Cu films were obtained via precession-assisted electron diffraction in the transmission electron microscope. These orientation images were used to characterize grain boundaries and to measure the average grain size of a microstructure, with and without considering twin boundaries. The results of these studies indicate that the contribution from grain-boundary scattering is the dominant factor (as compared to surface scattering) leading to enhanced resistivity. The resistivity data can be well-described by the combined Fuchs–Sondheimer surface scattering model and Mayadas–Shatzkes grain-boundary scattering model using Matthiessen's rule with a surface specularity coefficient of p?=?0.48 and a grain-boundary reflection coefficient of R?=?0.26.

  10. A quantitative comparison of sRNA-based and protein-based gene regulation

    E-Print Network [OSTI]

    Pankaj Mehta; Sidhartha Goyal; Ned S. Wingreen

    2008-09-02

    Small, non-coding RNAs (sRNAs) play important roles as genetic regulators in prokaryotes. sRNAs act post-transcriptionally via complementary pairing with target mRNAs to regulate protein expression. We use a quantitative approach to compare and contrast sRNAs with conventional transcription factors (TFs) to better understand the advantages of each form of regulation. In particular, we calculate the steady-state behavior, noise properties, frequency-dependent gain (amplification), and dynamical response to large input signals of both forms of regulation. While the mean steady-state behavior of sRNA-regulated proteins exhibits a distinctive tunable threshold-linear behavior, our analysis shows that transcriptional bursting leads to significantly higher intrinsic noise in sRNA-based regulation than in TF-based regulation in a large range of expression levels and limits the ability of sRNAs to perform quantitative signaling. Nonetheless, we find that sRNAs are better than TFs at filtering noise in input signals. Additionally, we find that sRNAs allow cells to respond rapidly to large changes in input signals. These features suggest a niche for sRNAs in allowing cells to transition quickly yet reliably between distinct states. This functional niche is consistent with the widespread appearance of sRNAs in stress-response and quasi-developmental networks in prokaryotes.

  11. Effect of temperature on quantitative structure/reactivity correlations for hydrocracking polynuclear aromatics

    SciTech Connect (OSTI)

    Korre, S.C.; Klein, M.T. [Univ. of Delaware, Newark, DE (United States); Quann, R.J. [Mobil Research and Development Corp., Paulsboro, NJ (United States)

    1995-12-31

    The hydrocracking reactions of phenanthrene and naphthalene were studied over a NiW/USY catalyst in a 1L batch autoclave at 68.1 bar hydrogen partial pressure, in the temperature interval 300{degrees}C-370{degrees}C. Parameter estimation to their hydrocracking pathways using a Langmuir-Hinshelwood-Hougen-Watson rate expression led to quantitative reaction networks. The variation in temperature offered information on 576 Arrhenius and van`t`Hoff parameters for rate, equilibrium, and adsorption constants. The number of parameters was reduced by the development of temperature-independent structure/reactivity correlations. A positive correlation was revealed between activation energies and pre-exponential factors for both isomerization and ring opening reactions. A compensation correlation for the calculation of standard isomerization and ring opening entropies was formulated based on the corresponding standard enthalpies. A total of 576 rate, equilibrium and adsorption parameters were thus summarized into just 18 Quantitative Structure/Reactivity Correlations parameters. Such correlations may provide a reasonable first-order estimate of parameters for molecules with known structure but unknown reactivity, with the use of simple semi-empirical molecular orbital calculations.

  12. Quantitative Reflectance Spectra of Solid Powders as a Function of Particle Size

    SciTech Connect (OSTI)

    Myers, Tanya L.; Brauer, Carolyn S.; Su, Yin-Fong; Blake, Thomas A.; Tonkyn, Russell G.; Ertel, Alyssa B.; Johnson, Timothy J.; Richardson, Robert L.

    2015-01-01

    We have recently developed vetted methods for obtaining quantitative infrared directional-hemispherical reflectance spectra using a commercial integrating sphere. In this paper, the effects of particle size on the spectral properties are analyzed for several samples such as ammonium sulfate, calcium carbonate, and sodium sulfate as well as one organic compound, lactose. We prepared multiple size fractions for each sample and confirmed the mean sizes using optical microscopy. Most species displayed a wide range of spectral behavior depending on the mean particle size. General trends of reflectance vs. particle size are observed such as increased albedo for smaller particles: for most wavelengths, the reflectivity drops with increased size, sometimes displaying a factor of 4 or more drop in reflectivity along with a loss of spectral contrast. In the longwave infrared, several species with symmetric anions or cations exhibited reststrahlen features whose amplitude was nearly invariant with particle size, at least for intermediate- and large-sized sample fractions; that is, > ~150 microns. Trends of other types of bands (Christiansen minima, transparency features) are also investigated as well as quantitative analysis of the observed relationship between reflectance vs. particle diameter.

  13. New facility design and work method for the quantitative fit testing laboratory. Master's thesis

    SciTech Connect (OSTI)

    Ward, G.F.

    1989-05-01

    The United States Air Force School of Aerospace Medicine (USAFSAM) tests the quantitative fit of masks which are worn by military personnel during nuclear, biological, and chemical warfare. Subjects are placed in a Dynatech-Frontier Fit Testing Chamber, salt air is fed into the chamber, and samples of air are drawn from the mask and the chamber. The ratio of salt air outside the mask to salt air inside the mask is called the quantitative fit factor. A motion-time study was conducted to evaluate the efficiency of the layout and work method presently used in the laboratory. A link analysis was done to determine equipment priorities, and the link data and design guidelines were used to develop three proposed laboratory designs. The proposals were evaluated by projecting the time and motion efficiency, and the energy expended working in each design. Also evaluated were the lengths of the equipment links for each proposal, and each proposal's adherence to design guidelines. A mock-up was built of the best design proposal, and a second motion-time study was run. Results showed that with the new laboratory and work procedures, the USAFSAM analyst could test 116 more subjects per year than are currently tested. Finally, the results of a questionnaire given to the analyst indicated that user acceptance of the work area improved with the new design.

  14. New Tool Quantitatively Maps Minority-Carrier Lifetime of Multicrystalline Silicon Bricks (Fact Sheet)

    SciTech Connect (OSTI)

    Not Available

    2011-11-01

    NREL's new imaging tool could provide manufacturers with insight on their processes. Scientists at the National Renewable Energy Laboratory (NREL) have used capabilities within the Process Development and Integration Laboratory (PDIL) to generate quantitative minority-carrier lifetime maps of multicrystalline silicon (mc-Si) bricks. This feat has been accomplished by using the PDIL's photoluminescence (PL) imaging system in conjunction with transient lifetime measurements obtained using a custom NREL-designed resonance-coupled photoconductive decay (RCPCD) system. PL imaging can obtain rapid high-resolution images that provide a qualitative assessment of the material lifetime-with the lifetime proportional to the pixel intensity. In contrast, the RCPCD technique provides a fast quantitative measure of the lifetime with a lower resolution and penetrates millimeters into the mc-Si brick, providing information on bulk lifetimes and material quality. This technique contrasts with commercially available minority-carrier lifetime mapping systems that use microwave conductivity measurements. Such measurements are dominated by surface recombination and lack information on the material quality within the bulk of the brick. By combining these two complementary techniques, we obtain high-resolution lifetime maps at very fast data acquisition times-attributes necessary for a production-based diagnostic tool. These bulk lifetime measurements provide manufacturers with invaluable feedback on their silicon ingot casting processes. NREL has been applying the PL images of lifetime in mc-Si bricks in collaboration with a U.S. photovoltaic industry partner through Recovery Act Funded Project ARRA T24. NREL developed a new tool to quantitatively map minority-carrier lifetime of multicrystalline silicon bricks by using photoluminescence imaging in conjunction with resonance-coupled photoconductive decay measurements. Researchers are not hindered by surface recombination and can look deeper into the material to map bulk lifetimes. The tool is being applied to silicon bricks in a project collaborating with a U.S. photovoltaic industry partner. Photovoltaic manufacturers can use the NREL tool to obtain valuable feedback on their silicon ingot casting processes.

  15. Quantitative degenerate four-wave mixing spectroscopy: Probes for molecular species

    SciTech Connect (OSTI)

    Farrow, R.; Rakestraw, D.; Paul, P.; Lucht, R.; Danehy, P.; Friedman-Hill, E.; Germann, G. [Sandia National Laboratories, Livermore, CA (United States)

    1993-12-01

    Resonant degenerate four-wave mixing (DFWM) is currently the subject of intensive investigation as a sensitive diagnostic tool for molecular species. DFWM has the advantage of generating a coherent (beam-like) signal which results in null-background detection and provides excellent immunity to background-light interference. Since multiple one-photon resonances are involved in the signal generation process, the DFWM technique can allow sensitive detection of molecules via electronic, vibrational or rotational transitions. These properties combine to make DFWM a widely applicable diagnostic technique for the probing of molecular species. The authors are conducting fundamental and applied investigations of DFWM for quantitative measurements of trace species in reacting gases. During the past year, efforts have been focussed in two areas: (1) understanding the effects of collisional processes on the DFWM signal generation process, and (2) exploring the applicability of infrared DFWM to detect polyatomic molecules via rovibrational transitions.

  16. DanteR: an extensible R-based tool for quantitative analysis of -omics data

    SciTech Connect (OSTI)

    Taverner, Thomas; Karpievitch, Yuliya; Polpitiya, Ashoka D.; Brown, Joseph N.; Dabney, Alan R.; Anderson, Gordon A.; Smith, Richard D.

    2012-09-15

    Motivation: The size and complex nature of LC-MS proteomics data sets motivates development of specialized software for statistical data analysis and exploration. We present DanteR, a graphical R package that features extensive statistical and diagnostic functions for quantitative proteomics data analysis, including normalization, imputation, hypothesis testing, interactive visualization and peptide-to-protein rollup. More importantly, users can easily extend the existing functionality by including their own algorithms under the Add-On tab. Availability: DanteR and its associated user guide are available for download at http://omics.pnl.gov/software/. For Windows, a single click automatically installs DanteR along with the R programming environment. For Linux and Mac OS X, users must first install R and then follow instructions on the DanteR web site for package installation.

  17. Quantitative description of ion transport via plasma membrane of yeast and small cells

    E-Print Network [OSTI]

    Vadim Volkov

    2012-12-18

    Modelling of ion transport via plasma membrane needs identification and quantitative understanding of the involved processes. Brief characterisation of ion transport systems of a yeast cell (Pma1, Ena1, TOK1, Nha1, Trk1, Trk2, non-selective cation conductance) and estimates concerning the number of molecules of each transporter per a cell allow predicting the corresponding ion flows. Comparison of ion transport in small yeast cell and several animal cell types is provided and importance of cell volume to surface ratio is stressed. Role of cell wall and lipid rafts is discussed in aspect of required increase in spatial and temporary resolution of measurements. Conclusions are formulated to describe specific features of ion transport in a yeast cell. Potential directions of future research are outlined based on the assumptions.

  18. Quantitative Cyber Risk Reduction Estimation Methodology for a Small Scada Control System

    SciTech Connect (OSTI)

    Miles A. McQueen; Wayne F. Boyer; Mark A. Flynn; George A. Beitel

    2006-01-01

    We propose a new methodology for obtaining a quick quantitative measurement of the risk reduction achieved when a control system is modified with the intent to improve cyber security defense against external attackers. The proposed methodology employs a directed graph called a compromise graph, where the nodes represent stages of a potential attack and the edges represent the expected time-to-compromise for differing attacker skill levels. Time-to-compromise is modeled as a function of known vulnerabilities and attacker skill level. The methodology was used to calculate risk reduction estimates for a specific SCADA system and for a specific set of control system security remedial actions. Despite an 86% reduction in the total number of vulnerabilities, the estimated time-to-compromise was increased only by about 3 to 30% depending on target and attacker skill level.

  19. Quantitative, Comparable Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy: Correcting Errors in Phase Retrieval

    E-Print Network [OSTI]

    Camp, Charles H; Cicerone, Marcus T

    2015-01-01

    Coherent anti-Stokes Raman scattering (CARS) microspectroscopy has demonstrated significant potential for biological and materials imaging. To date, however, the primary mechanism of disseminating CARS spectroscopic information is through pseudocolor imagery, which explicitly neglects a vast majority of the hyperspectral data. Furthermore, current paradigms in CARS spectral processing do not lend themselves to quantitative sample-to-sample comparability. The primary limitation stems from the need to accurately measure the so-called nonresonant background (NRB) that is used to extract the chemically-sensitive Raman information from the raw spectra. Measurement of the NRB on a pixel-by-pixel basis is a nontrivial task; thus, reference NRB from glass or water are typically utilized, resulting in error between the actual and estimated amplitude and phase. In this manuscript, we present a new methodology for extracting the Raman spectral features that significantly suppresses these errors through phase detrending ...

  20. Quantitative similarity analysis of small-break loss-of-coolant accident scenarios

    SciTech Connect (OSTI)

    Prosek, A.; Kljenak, I.; Mavko, B. [Univ. of Ljubljana (Slovenia). Jozef Stefan Inst.

    1996-07-01

    Classifications of small-break loss-of-coolant accidents based on objective quantitative similarity analysis are proposed. Accident scenarios were simulated in a two-loop pressurized water reactor plant with the RELAP5/MOD3.1 computer code for break sizes ranging from 1.27 cm (0.5 in.) to 15.2 cm (6 in.), with different availability of auxiliary feedwater system or reactor coolant pump trip delay. Similarities between different accident simulations were evaluated by comparing relevant time-dependent parameters with fast Fourier transform and correlation methods. Quantification of similarity between accident simulations could eventually lead to further development of the Code Scaling, Applicability and Uncertainty methodology.

  1. Effect of temperature on quantitative structure/reactivity relationships for hydrocracking polynuclear aromatics

    SciTech Connect (OSTI)

    Korre, S.C.; Klein, M.T. [Univ. of Delaware, Newark, DE (United States)

    1995-12-31

    The increased interest in the molecular modeling of hydrocarbon conversion processes is motivated by the need to predict both the performance and environmental properties of the products. A molecular description of the hydrocarbon mixture is the logical starting point for these predictions, which has motivated considerable interest in the development of molecular reaction models. The construction of such models is complicated by the enormous need for rate, adsorption and equilibrium parameters. This staggering need can be reduced considerably when the homologous patterns of both compound types and their reactions in these mixtures can be recognized. This has motivated the development of a lumping approach that organizes the kinetics information in potentially thousands of rate, adsorption and equilibrium constants into the parameters of a handful (O(10)) of Quantitative Structure/Reactivity Relationships (QSRR) using the formalism of Linear Free Energy Relationships. This paper describes the hydrocracking of phenanthrene and naphthalene.

  2. Quantitative comparison of fuel spray images obtained using ultrafast coherent and incoherent double-pulsed illumination

    E-Print Network [OSTI]

    Purwar, Harsh; Idlahcen, Saďd; Rozé, Claude; Blaisot, Jean-Bernard; Ménard, Thibault

    2015-01-01

    We present a quantitative comparison between the high-pressure fuel spray images obtained experimentally using classical imaging with coherent and incoherent ultrafast illuminations recorded using a compatible CMOS camera. The ultrafast, incoherent illumination source was extracted from the supercontinuum generated by tightly focusing the femtosecond laser pulses in water. The average velocity maps computed using time-correlated image-pairs and spray edge complexity computed using the average curvature scale space maps are compared for the spray images obtained with the two illumination techniques and also for the numerically simulated spray using the coupled volume of fluid and level set method for interface tracking (direct numerical simulation or DNS). The spray images obtained with supercontinuum-derived, incoherent, ultrafast illumination are clearer, since the artifacts arising due to laser speckles and multiple diffraction effects are largely reduced and show a better correlation with the DNS results.

  3. Active illumination using a digital micromirror device for quantitative phase imaging

    E-Print Network [OSTI]

    Shin, Seungwoo; Yoon, Jonghee; Park, YongKeun

    2015-01-01

    We present a powerful and cost-effective method for active illumination using a digital micromirror device (DMD) for quantitative phase imaging techniques. Displaying binary illumination patterns on a DMD with appropriate spatial filtering, plane waves with various illumination angles are generated and impinged onto a sample. Complex optical fields of the sample obtained with various incident angles are then measured via Mach-Zehnder interferometry, from which a high-resolution two-dimensional synthetic aperture phase image and a three-dimensional refractive index tomogram of the sample are reconstructed. We demonstrate the fast and stable illumination control capability of the proposed method by imaging colloidal spheres and biological cells, including a human red blood cell and a HeLa cell.

  4. Quantitative PCR Analysis of Molds in the Dust from Homes of Asthmatic Children in North Carolina

    SciTech Connect (OSTI)

    Vesper, Stephen J.; McKinstry, Craig A.; Ashley, Peter; Haugland, Richard A.; Yeatts, Karin; Bradham, Karen; Svendsen, Eric

    2007-07-10

    The vacuum cleaner bag (VCB) dust from the homes of 19 asthmatic children in North Carolina (NC) was analyzed by mold specific quantitative PCR. These results were compared to the analysis of the VCB dust from 157 homes in the HUD “American Healthy Home Survey” of homes in the US. The American Relative Moldiness Index (ARMI) was calculated for each of the homes. The mean and standard deviation (SD) of the ARMI values in the homes of the NC asthmatic children was 11.0 (5.3), compared to the HUD survey VCB ARMI value mean and SD of 6.6 (4.4). The median ARMI value was significantly higher(p < 0.001) in the asthmatic childrens’s homes. The molds Chaetomium globosum and Eurotium amsterdameli were the primary species in the NC homes making the ARMI values higher. Vacuum cleaner bag dust samples may be a less expensive but still useful method of home mold analysis.

  5. Quantitative analysis of the evolution of novelty in cinema through crowdsourced keywords

    E-Print Network [OSTI]

    Sreenivasan, Sameet

    2013-01-01

    The generation of novelty is central to any creative endeavor. Novelty generation and the relationship between novelty and individual hedonic value have long been subjects of study in social psychology. However, few studies have utilized large-scale datasets to quantitatively investigate these issues. Here we consider the domain of American cinema and explore these questions using a database of films spanning a 70 year period. We use crowdsourced keywords from the Internet Movie Database as a window into the contents of films, and prescribe novelty scores for each film based on occurrence probabilities of individual keywords and keyword-pairs. These scores provide revealing insights into the dynamics of novelty in cinema. We investigate how novelty influences the revenue generated by a film, and find a statistically significant relationship that resembles the Wundt-Berlyne curve. We also study the statistics of keyword occurrence and the aggregate distribution of keywords over a 100 year period.

  6. Quantitative multiplex detection of biomarkers on a waveguide-based biosensor using quantum dots

    SciTech Connect (OSTI)

    Xie, Hongzhi; Mukundan, Harshini; Martinez, Jennifer S; Swanson, Basil I; Anderson, Aaron S; Grace, Kevin

    2009-01-01

    The quantitative, simultaneous detection of multiple biomarkers with high sensitivity and specificity is critical for biomedical diagnostics, drug discovery and biomarker characterization [Wilson 2006, Tok 2006, Straub 2005, Joos 2002, Jani 2000]. Detection systems relying on optical signal transduction are, in general, advantageous because they are fast, portable, inexpensive, sensitive, and have the potential for multiplex detection of analytes of interest. However, conventional immunoassays for the detection of biomarkers, such as the Enzyme Linked Immunosorbant Assays (ELISAs) are semi-quantitative, time consuming and insensitive. ELISA assays are also limited by high non-specific binding, especially when used with complex biological samples such as serum and urine (REF). Organic fluorophores that are commonly used in such applications lack photostability and possess a narrow Stoke's shift that makes simultaneous detection of multiple fluorophores with a single excitation source difficult, thereby restricting their use in multiplex assays. The above limitations with traditional assay platforms have resulted in the increased use of nanotechnology-based tools and techniques in the fields of medical imaging [ref], targeted drug delivery [Caruthers 2007, Liu 2007], and sensing [ref]. One such area of increasing interest is the use of semiconductor quantum dots (QDs) for biomedical research and diagnostics [Gao and Cui 2004, Voura 2004, Michalet 2005, Chan 2002, Jaiswal 2004, Gao 2005, Medintz 2005, So 2006 2006, Wu 2003]. Compared to organic dyes, QDs provide several advantages for use in immunoassay platforms, including broad absorption bands with high extinction coefficients, narrow and symmetric emission bands with high quantum yields, high photostablility, and a large Stokes shift [Michalet 2005, Gu 2002]. These features prompted the use of QDs as probes in biodetection [Michalet 2005, Medintz 2005]. For example, Jaiswal et al. reported long term multiple color imaging of live cells using QD-bioconjugates [Jaiswal 2003]. Gao [Gao 2004] and So [So 2006] have used QDs as probes for in-vivo cancer targeting and imaging. Medintz et al. reported self-assembled QD-based biosensors for detection of analytes based on energy transfer [Medintz 2003]. Others have developed an approach for multiplex optical encoding of biomolecules using QDs [Han 2001]. Immunoassays have also benefited from the advantages of QDs. Recently, dihydrolipoic acid (DHLA) capped-QDs have been attached to antibodies and used as fluorescence reporters in plate-based multiplex immunoassays [Goodman 2004]. However, DHLA-QDs are associated with low quantum efficiency and are unstable at neutral pH. These problems limit the application of this technology to the sensitive detection of biomolecules, especially in complex biological samples. Thus, the development of a rapid, sensitive, quantitative, and specific multiplex platform for the detection of biomarkers in difficult samples remains an elusive target. The goal stated above has applications in many fields including medical diagnostics, biological research, and threat reduction. The current decade alone has seen the development of a need to rapidly and accurately detect potential biological warfare agents. For example, current methods for the detection of anthrax are grossly inadequate for a variety of reasons including long incubation time (5 days from time of exposure to onset of symptoms) and non-specific ('flu-like') symptoms. When five employees of the United State Senate were exposed to B. anthracis in the mail (2001), only one patient had a confirmed diagnosis before death. Since then, sandwich immunoassays using both colorimetric and fluorescence detectors have been developed for key components of the anthrax lethal toxin, namely protective antigen (PA), lethal factor (LF), and the edema factor [Mourez 2001]. While these platforms were successful in assays against anthrax toxins, the sensitivity was poor. Furthermore, no single platform exists for the simultaneous and quantitative detection of mul

  7. Shotgun Approach for Quantitative Imaging of Phospholipids Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Thomas, Mathew; Laskin, Julia

    2014-02-04

    Mass spectrometry imaging (MSI) has been extensively used for determining spatial distributions of molecules in biological samples, and there is increasing interest in using MSI for quantification. Nanospray desorption electrospray ionization, or nano-DESI, is an ambient MSI technique where a solvent is used for localized extraction of molecules followed by nanoelectrospray ionization. Doping the nano-DESI solvent with carefully selected standards enables online quantification during MSI experiments. In this proof-of-principle study, we demonstrate this quantification approach can be extended to provide shotgun-like quantification of phospholipids in thin brain tissue sections. Specifically, two phosphatidylcholine (PC) standards were added to the nano-DESI solvent for simultaneous imaging and quantification of 22 PC species observed in nano-DESI MSI. Furthermore, by combining the quantitative data obtained in the individual pixels, we demonstrate quantification of these PC species in seven different regions of a rat brain tissue section.

  8. ANSI/ASHRAE/IES Standard 90.1-2013 Determination of Energy Savings: Quantitative Analysis

    SciTech Connect (OSTI)

    Halverson, Mark A.; Athalye, Rahul A.; Rosenberg, Michael I.; Xie, YuLong; Wang, Weimin; Hart, Philip R.; Zhang, Jian; Goel, Supriya; Mendon, Vrushali V.

    2014-09-04

    This report provides a final quantitative analysis to assess whether buildings constructed according to the requirements of ANSI/ASHRAE/IES Standard 90.1-2013 would result in improved energy efficiency in commercial buildings. The final analysis considered each of the 110 addenda to Standard 90.1-2010 that were included in Standard 90.1-2013. PNNL reviewed all addenda included by ASHRAE in creating Standard 90.1-2013 from Standard 90.1-2010, and considered their combined impact on a suite of prototype building models across all U.S. climate zones. Most addenda were deemed to have little quantifiable impact on building efficiency for the purpose of DOE’s final determination. However, out of the 110 total addenda, 30 were identified as having a measureable and quantifiable impact.

  9. Standard test method for quantitative determination of americium 241 in plutonium by Gamma-Ray spectrometry

    E-Print Network [OSTI]

    American Society for Testing and Materials. Philadelphia

    1994-01-01

    1.1 This test method covers the quantitative determination of americium 241 by gamma-ray spectrometry in plutonium nitrate solution samples that do not contain significant amounts of radioactive fission products or other high specific activity gamma-ray emitters. 1.2 This test method can be used to determine the americium 241 in samples of plutonium metal, oxide and other solid forms, when the solid is appropriately sampled and dissolved. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

  10. Quantitative assessment of electrostatic embedding in Density Functional Theory calculations of biomolecular systems

    SciTech Connect (OSTI)

    Fattebert, J; Law, R J; Bennion, B; Lau, E Y; Schwegler, E; Lightstone, F C

    2009-04-24

    We evaluate the accuracy of density functional theory quantum calculations of biomolecular subsystems using a simple electrostatic embedding scheme. Our scheme is based on dividing the system of interest into a primary and secondary subsystem. A finite difference discretization of the Kohn-Sham equations is used for the primary subsystem, while its electrostatic environment is modeled with a simple one-electron potential. Force-field atomic partial charges are used to generate smeared Gaussian charge densities and to model the secondary subsystem. We illustrate the utility of this approach with calculations of truncated dipeptide chains. We analyze quantitatively the accuracy of this approach by calculating atomic forces and comparing results with fullQMcalculations. The impact of the choice made in terminating dangling bonds at the frontier of the QM region is also investigated.

  11. The Food Crises: A quantitative model of food prices including speculators and ethanol conversion

    E-Print Network [OSTI]

    Lagi, Marco; Bertrand, Karla Z; Bar-Yam, Yaneer

    2011-01-01

    Recent increases in basic food prices are severely impacting vulnerable populations worldwide. Proposed causes such as shortages of grain due to adverse weather, increasing meat consumption in China and India, conversion of corn to ethanol in the US, and investor speculation on commodity markets lead to widely differing implications for policy. A lack of clarity about which factors are responsible reinforces policy inaction. Here, for the first time, we construct a dynamic model that quantitatively agrees with food prices. The results show that the dominant causes of price increases are investor speculation and ethanol conversion. Models that just treat supply and demand are not consistent with the actual price dynamics. The two sharp peaks in 2007/2008 and 2010/2011 are specifically due to investor speculation, while an underlying upward trend is due to increasing demand from ethanol conversion. The model includes investor trend following as well as shifting between commodities, equities and bonds to take ad...

  12. Quantitative analysis of directional spontaneous emission spectra from light sources in photonic crystals

    SciTech Connect (OSTI)

    Nikolaev, Ivan S.; Lodahl, Peter; Vos, Willem L.

    2005-05-15

    We have performed angle-resolved measurements of spontaneous-emission spectra from laser dyes and quantum dots in opal and inverse opal photonic crystals. Pronounced directional dependencies of the emission spectra are observed: angular ranges of strongly reduced emission adjoin with angular ranges of enhanced emission. It appears that emission from embedded light sources is affected both by the periodicity and by the structural imperfections of the crystals: the photons are Bragg diffracted by lattice planes and scattered by unavoidable structural disorder. Using a model comprising diffuse light transport and photonic band structure, we quantitatively explain the directional emission spectra. This work provides detailed understanding of the transport of spontaneously emitted light in real photonic crystals, which is essential in the interpretation of quantum optics in photonic-band-gap crystals and for applications wherein directional emission and total emission power are controlled.

  13. Quantitative measurement of binary liquid distributions using multiple-tracer x-ray fluorescence and radiography

    SciTech Connect (OSTI)

    Halls, Benjamin R.; Meyer, Terrence R.; Kastengren, Alan L.

    2015-01-01

    The complex geometry and large index-of-refraction gradients that occur near the point of impingement of binary liquid jets present a challenging environment for optical interrogation. A simultaneous quadruple-tracer x-ray fluorescence and line-of-sight radiography technique is proposed as a means of distinguishing and quantifying individual liquid component distributions prior to, during, and after jet impact. Two different pairs of fluorescence tracers are seeded into each liquid stream to maximize their attenuation ratio for reabsorption correction and differentiation of the two fluids during mixing. This approach for instantaneous correction of x-ray fluorescence reabsorption is compared with a more time-intensive approach of using stereographic reconstruction of x-ray attenuation along multiple lines of sight. The proposed methodology addresses the need for a quantitative measurement technique capable of interrogating optically complex, near-field liquid distributions in many mixing systems of practical interest involving two or more liquid streams.

  14. Quantitative measurement of binary liquid distributions using multiple-tracer x-ray fluorescence and radiography

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Halls, Benjamin R.; Meyer, Terrence R.; Kastengren, Alan L.

    2015-01-23

    The complex geometry and large index-of-refraction gradients that occur near the point of impingement of binary liquid jets present a challenging environment for optical interrogation. A simultaneous quadruple-tracer x-ray fluorescence and line-of-sight radiography technique is proposed as a means of distinguishing and quantifying individual liquid component distributions prior to, during, and after jet impact. Two different pairs of fluorescence tracers are seeded into each liquid stream to maximize their attenuation ratio for reabsorption correction and differentiation of the two fluids during mixing. This approach for instantaneous correction of x-ray fluorescence reabsorption is compared with a more time-intensive approach of usingmore »stereographic reconstruction of x-ray attenuation along multiple lines of sight. The proposed methodology addresses the need for a quantitative measurement technique capable of interrogating optically complex, near-field liquid distributions in many mixing systems of practical interest involving two or more liquid streams.« less

  15. Quantitative Proteomic Profiling of Low Dose Ionizing Radiation Effects in a Human Skin Model

    SciTech Connect (OSTI)

    Hengel, Shawna; Aldrich, Joshua T.; Waters, Katrina M.; Pasa-Tolic, Ljiljana; Stenoien, David L.

    2014-07-29

    To assess molecular responses to low doses of radiation that may be encountered during medical diagnostic procedures, nuclear accidents, or terrorist acts, a quantitative global proteomic approach was used to identify protein alterations in a reconstituted human skin tissue treated with 10 cGy of ionizing radiation. Subcellular fractionation was employed to remove highly abundant structural proteins and provide insight on radiation induced alterations in protein abundance and localization. In addition, peptides were post-fractionated using high resolution 2-dimensional liquid chromatography to increase the dynamic range of detection of protein abundance and translocation changes. Quantitative data was obtained by labeling peptides with 8-plex isobaric iTRAQ tags. A total of 207 proteins were detected with statistically significant alterations in abundance and/or subcellular localization compared to sham irradiated tissues. Bioinformatics analysis of the data indicated that the top canonical pathways affected by low dose radiation are related to cellular metabolism. Among the proteins showing alterations in abundance, localization and proteolytic processing was the skin barrier protein filaggrin which is consistent with our previous observation that ionizing radiation alters profilaggrin processing with potential effects on skin barrier functions. In addition, a large number of proteases and protease regulators were affected by low dose radiation exposure indicating that altered proteolytic activity may be a hallmark of low dose radiation exposure. While several studies have demonstrated altered transcriptional regulation occurs following low dose radiation exposures, the data presented here indicates post-transcriptional regulation of protein abundance, localization, and proteolytic processing play an important role in regulating radiation responses in complex human tissues.

  16. Quantitative comparison of the in situ microbial communities in different biomes

    SciTech Connect (OSTI)

    White, D.C. |; Ringelberg, D.B.; Palmer, R.J.

    1995-12-31

    A system to define microbial communities in different biomes requires the application of non-traditional methodology. Classical microbiological methods have severe limitations for the analysis of environmental samples. Pure-culture isolation, biochemical testing, and/or enumeration by direct microscopic counting are not well suited for the estimation of total biomass or the assessment of community composition within environmental samples. Such methods provide little insight into the in situ phenotypic activity of the extant microbiota since these techniques are dependent on microbial growth and thus select against many environmental microorganisms which are non- culturable under a wide range of conditions. It has been repeatedly documented in the literature that viable counts or direct counts of bacteria attached to sediment grains are difficult to quantitative and may grossly underestimate the extent of the existing community. The traditional tests provide little indication of the in situ nutritional status or for evidence of toxicity within the microbial community. A more recent development (MIDI Microbial Identification System), measure free and ester-linked fatty acids from isolated microorganisms. Bacterial isolates are identified by comparing their fatty acid profiles to the MIKI database which contains over 8000 entries. The application of the MIKI system to the analysis of environmental samples however, has significant drawbacks. The MIDI system was developed to identify clinical microorganisms and requires their isolation and culture on trypticase soy agar at 27{degrees}C. Since many isolates are unable to grow at these restrictive growth conditions, the system does not lend itself to identification of some environmental organisms. A more applicable methodology for environmental microbial analysis is based on the liquid extrication and separation of microbial lipids from environmental samples, followed by quantitative analysis using gas chromatography/

  17. Use of Quantitative Uncertainty Analysis to Support M&VDecisions in ESPCs

    SciTech Connect (OSTI)

    Mathew, Paul A.; Koehling, Erick; Kumar, Satish

    2005-05-11

    Measurement and Verification (M&V) is a critical elementof an Energy Savings Performance Contract (ESPC) - without M&V, thereisno way to confirm that the projected savings in an ESPC are in factbeing realized. For any given energy conservation measure in an ESPC,there are usually several M&V choices, which will vary in terms ofmeasurement uncertainty, cost, and technical feasibility. Typically,M&V decisions are made almost solely based on engineering judgmentand experience, with little, if any, quantitative uncertainty analysis(QUA). This paper describes the results of a pilot project initiated bythe Department of Energy s Federal Energy Management Program to explorethe use of Monte-Carlo simulation to assess savings uncertainty andthereby augment the M&V decision-making process in ESPCs. The intentwas to use QUA selectively in combination with heuristic knowledge, inorder to obtain quantitative estimates of the savings uncertainty withoutthe burden of a comprehensive "bottoms-up" QUA. This approach was used toanalyze the savings uncertainty in an ESPC for a large federal agency.The QUA was seamlessly integrated into the ESPC development process andthe incremental effort was relatively small with user-friendly tools thatare commercially available. As the case study illustrates, in some casesthe QUA simply confirms intuitive or qualitative information, while inother cases, it provides insight that suggests revisiting the M&Vplan. The case study also showed that M&V decisions should beinformed by the portfolio risk diversification. By providing quantitativeuncertainty information, QUA can effectively augment the M&Vdecision-making process as well as the overall ESPC financialanalysis.

  18. Parameterized algorithms for quantitative differentials in spectrally equivalent medical diagnostic x-ray beams

    SciTech Connect (OSTI)

    Okunade, Akintunde Akangbe [Department of Physics, Obafemi Awolowo University, Ile-Ife, Osun State (Nigeria)

    2005-06-15

    Qualitative and quantitative equivalence of spectra transmitted by two different elemental filters require a good match in terms of shape and size over the entire energy range of 0-150 keV used in medical diagnostic radiology. However, the photoelectric absorptions and Compton scattering involved in the interaction of x rays with matter at these relatively low photon energies differ in a nonuniform manner with energy and atomic number. By careful choice of thicknesses for filter materials with an atomic number between 12 and 39, when compared with aluminum, it is possible to obtain transmitted beams of the same shape (quality) but not of the same size (quantity). In this paper, calculations have been carried out for the matching of the shapes and sizes of beams transmitted through specified thicknesses of aluminium filter and spectrally equivalent thicknesses of other filter materials (different from aluminium) using FORTRAN source codes traceable to the American Association of Physics in Medicine (AAPM), College Park, MD, USA. Parametrized algorithms for the evaluation of quantitative differentials (deficit or surplus) in radiation output (namely, photon fluence, exposure, kerma, energy imparted, absorbed dose, and effective dose) from these transmitted spectrally equivalent beams were developed. These differentials range between 1%, and 4% at 1 mm Al filtration and between 8%, and 25% for filtration of 6 mm Al for different filter materials in comparison with aluminum. Also developed were models for factors for converting measures of photon fluence, exposure-area product, (EAP), and kerma-area product (KAP) to risk related quantities such as energy imparted, absorbed dose, and effective dose from the spectrally equivalent beams. The thicknesses of other filter materials that are spectrally equivalent to given thicknesses of aluminum filter were characterized using polynomial functions. The fact that the use of equivalent spectra in radiological practice can provide means of ranking the differentials in radiographic image quality and stochastic risk is discussed.

  19. Quantitative assessment of biological impact using transcriptomic data and mechanistic network models

    SciTech Connect (OSTI)

    Thomson, Ty M. [Selventa, One Alewife Center, Cambridge, MA 02140 (United States); Sewer, Alain, E-mail: Alain.Sewer@pmi.com [Philip Morris International R and D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel (Switzerland); Martin, Florian; Belcastro, Vincenzo [Philip Morris International R and D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel (Switzerland); Frushour, Brian P. [Selventa, One Alewife Center, Cambridge, MA 02140 (United States); Gebel, Stephan [Philip Morris International R and D, Philip Morris Research Laboratories GmbH, Edmund-Rumpler-Strasse 5, 51149 Koeln (Germany); Park, Jennifer [Selventa, One Alewife Center, Cambridge, MA 02140 (United States); Schlage, Walter K. [Philip Morris International R and D, Philip Morris Research Laboratories GmbH, Edmund-Rumpler-Strasse 5, 51149 Koeln (Germany); Talikka, Marja [Philip Morris International R and D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel (Switzerland); Vasilyev, Dmitry M.; Westra, Jurjen W. [Selventa, One Alewife Center, Cambridge, MA 02140 (United States); Hoeng, Julia; Peitsch, Manuel C. [Philip Morris International R and D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel (Switzerland)

    2013-11-01

    Exposure to biologically active substances such as therapeutic drugs or environmental toxicants can impact biological systems at various levels, affecting individual molecules, signaling pathways, and overall cellular processes. The ability to derive mechanistic insights from the resulting system responses requires the integration of experimental measures with a priori knowledge about the system and the interacting molecules therein. We developed a novel systems biology-based methodology that leverages mechanistic network models and transcriptomic data to quantitatively assess the biological impact of exposures to active substances. Hierarchically organized network models were first constructed to provide a coherent framework for investigating the impact of exposures at the molecular, pathway and process levels. We then validated our methodology using novel and previously published experiments. For both in vitro systems with simple exposure and in vivo systems with complex exposures, our methodology was able to recapitulate known biological responses matching expected or measured phenotypes. In addition, the quantitative results were in agreement with experimental endpoint data for many of the mechanistic effects that were assessed, providing further objective confirmation of the approach. We conclude that our methodology evaluates the biological impact of exposures in an objective, systematic, and quantifiable manner, enabling the computation of a systems-wide and pan-mechanistic biological impact measure for a given active substance or mixture. Our results suggest that various fields of human disease research, from drug development to consumer product testing and environmental impact analysis, could benefit from using this methodology. - Highlights: • The impact of biologically active substances is quantified at multiple levels. • The systems-level impact integrates the perturbations of individual networks. • The networks capture the relationships between the biological mechanisms. • Four exposure experiments have been assessed to validate the methodology. • The impact results were consistent with the corresponding phenotypic measures.

  20. Apparatus and method for quantitatively evaluating total fissile and total fertile nuclide content in samples. [Patent application

    DOE Patents [OSTI]

    Caldwell, J.T.; Kunz, W.E.; Cates, M.R.; Franks, L.A.

    1982-07-07

    Simultaneous photon and neutron interrogation of samples for the quantitative determination of total fissile nuclide and total fertile nuclide material present is made possible by the use of an electron accelerator. Prompt and delayed neutrons produced from resulting induced fission are counted using a single detection system and allow the resolution of the contributions from each interrogating flux leading in turn to the quantitative determination sought. Detection limits for /sup 239/Pu are estimated to be about 3 mg using prompt fission neutrons and about 6 mg using delayed neutrons.

  1. Formation resistivity measurements from within a cased well used to quantitatively determine the amount of oil and gas present

    DOE Patents [OSTI]

    Vail, III, William B. (Bothell, WA)

    1997-01-01

    Methods to quantitatively determine the separate amounts of oil and gas in a geological formation adjacent to a cased well using measurements of formation resistivity are disclosed. The steps include obtaining resistivity measurements from within a cased well of a given formation, obtaining the porosity, obtaining the resistivity of formation water present, computing the combined amounts of oil and gas present using Archie's Equations, determining the relative amounts of oil and gas present from measurements within a cased well, and then quantitatively determining the separate amounts of oil and gas present in the formation.

  2. Formation resistivity measurements from within a cased well used to quantitatively determine the amount of oil and gas present

    DOE Patents [OSTI]

    Vail, W.B. III

    1997-05-27

    Methods to quantitatively determine the separate amounts of oil and gas in a geological formation adjacent to a cased well using measurements of formation resistivity are disclosed. The steps include obtaining resistivity measurements from within a cased well of a given formation, obtaining the porosity, obtaining the resistivity of formation water present, computing the combined amounts of oil and gas present using Archie`s Equations, determining the relative amounts of oil and gas present from measurements within a cased well, and then quantitatively determining the separate amounts of oil and gas present in the formation. 7 figs.

  3. Evaluation of a portable x-ray fluorescence survey meter for the quantitative determination of trace metals in welding fumes 

    E-Print Network [OSTI]

    Fehrenbacher, Mary Catherine

    1984-01-01

    EVALUATION OF A PORTABLE X-RAY FLUORE~ SURVEY METER FOR TIIE QUANTITATIVE DEPERMINATI(gq OF TRACE METALS IN WELDING FIJvtES A THESIS by MARY CATHERINE FEHRENBACHER Submitted to the Graduate College of Texas A%M University in partial... fulfillment of the requirement for the degree of MASTER OF SCIENCE May, 1984 MAJOR SUBJECI': INDUSTRIAL HYGIENE EVALUATION OF A PORTABLE X-RAY FLUORES~ SURVEY METER FOR THE QUANTITATIVE DETERMINATIGN OF TRACE METALS IN WELDING FIJvtES A THESIS by h...

  4. Assistant Professor in Quantitative Genomics POSITION: This is a 12-month tenure track faculty position with a 80% research and

    E-Print Network [OSTI]

    Arnold, Jonathan

    Assistant Professor in Quantitative Genomics POSITION: This is a 12-month tenure track faculty, statistical genomics, genome-wide analysis, gene-environment interactions, and/or epigenetics of crop plants/statistical genomics to crop plants is expected. UGA offers a vibrant research environment with potential

  5. Proceedings of the NARST 2004 Annual Meeting (Vancouver, BC, Canada) THE INFLUENCE OF WEB-BASED HOMEWORK ON QUANTITATIVE

    E-Print Network [OSTI]

    -BASED HOMEWORK ON QUANTITATIVE PROBLEM­SOLVING IN A UNIVERSITY PHYSICS CLASS Homework is often assigned on student problem-solving and makes a comparison to the use of traditional paper-pencil homework, videotaped problem-solving interviews. From the analysis of this data, problem-solving characterizations

  6. QUANTITATIVE REMOTE SENSING: HORNS REV WIND FARM CASE STUDY C. B. Hasager, M. Nielsen, M. B. Christiansen

    E-Print Network [OSTI]

    QUANTITATIVE REMOTE SENSING: HORNS REV WIND FARM CASE STUDY C. B. Hasager, M. Nielsen, M. B. Christiansen Risř National Laboratory, Wind Energy Department, Roskilde, Denmark Email: charlotte.hasager@risoe.dk, morten.nielsen@risoe.dk, merete.bruun.christiansen@risoe.dk ABSTRACT Wind vector observations from 85 ERS

  7. 2566 IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 36, NO. 5, OCTOBER 2008 Quantitative Analysis of Gas Circuit Breaker

    E-Print Network [OSTI]

    Basse, Nils Plesner

    2566 IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 36, NO. 5, OCTOBER 2008 Quantitative Analysis of Gas-circuit current interruption performance. In this paper, we study a single arc discharge both using measurements in the heating volume created by radiative ablation of the Polytetrafluoroethylene nozzles surrounding the arc

  8. CHINESE JOURNAL OF CHEMICAL PHYSICS VOLUME 22, NUMBER 6 DECEMBER 27, 2009 Quantitative Surface Chirality Detection with Sum Frequency Generation

    E-Print Network [OSTI]

    Liu, Shilin

    CHINESE JOURNAL OF CHEMICAL PHYSICS VOLUME 22, NUMBER 6 DECEMBER 27, 2009 ARTICLE Quantitative of Molecular Reaction Dynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China the chiral Part of the special issue for "the Chinese Chemical Society's 11th National Chemical Dynamics

  9. A 6000-year record of interaction between Hamilton Harbour and Lake Ontario: quantitative assessment of recent hydrologic

    E-Print Network [OSTI]

    Edwards, Thomas W.D.

    A 6000-year record of interaction between Hamilton Harbour and Lake Ontario: quantitative of Waterloo, Waterloo, Ontario Canada N2L 3G1 b Department of Biology, Quaternary Sciences Institute, University of Waterloo, Waterloo, Ontario Canada N2L 3G1 Abstract Hamilton Harbour, a heavily urbanized

  10. A Low-Cost Quantitative Absorption Spectrophotometer Daniel R. Albert, Michael A. Todt, and H. Floyd Davis*

    E-Print Network [OSTI]

    Davis, H. Floyd

    A Low-Cost Quantitative Absorption Spectrophotometer Daniel R. Albert, Michael A. Todt, and H with spectrophotometry. Additionally, more than 75% of the high school teachers polled stated that the high cost of modern spectrophotometers was prohibitive for regular classroom use. The lowest-cost modern

  11. Molecular Immunology 44 (2007) 30853091 Epi-allelic Erk1 and Erk2 knockdown series for quantitative analysis

    E-Print Network [OSTI]

    2007-01-01

    Molecular Immunology 44 (2007) 3085­3091 Epi-allelic Erk1 and Erk2 knockdown series for quantitative analysis of T cell Erk regulation and IL-2 production Lucia Willea,1, Melissa L. Kempb,1, Peter 2006; received in revised form 21 January 2007; accepted 7 February 2007 Abstract Erk activation

  12. Quantitative extraction and concentration of synthetic water-soluble acid dyes from aqueous media using a quinine-chloroform solution

    SciTech Connect (OSTI)

    Kobayashi, F.; Ozawa, N.; Hanai, J.; Isobe, M.; Watabe, T.

    1986-12-01

    Twenty-one water-soluble acid dyes, including eleven azo, five triphenylmethane four xanthene, one naphthol derivatives, used at practical concentrations for food coloration, were quantitatively extracted from water and various carbonated beverages into a 0.1 M quinine-chloroform solution in the presence of 0.5 M boric acid by brief shaking. Quantitative extraction of these dyes was also accomplished by the 0.1 M quinine-chloroform solution made conveniently from chloroform, quinine hydrochloride, and sodium hydroxide added successively to water or beverages containing boric acid. Quinine acted as a countercation on the dyes having sulfonic and/or carboxylic acid group(s) to form chloroform-soluble ion-pair complexes. The diacidic base alkaloid interacted with each acid group of mono-, di-, tri-, and tetrasulfonic acid dyes approximately in the ratio 0.8-0.9 to 1. The dyes in the chloroform solution were quantitatively concentrated into a small volume of sodium hydroxide solution also by brief shaking. The convenient quinine-chloroform method was applicable to the quantitative extraction of a mixture of 12 dyes from carbonated beverages, which are all currently used for food coloration. A high-pressure liquid chromatographic method is also presented for the systematic separation and determination of these 12 dyes following their concentration into the aqueous alkaline solution. The chromatogram was monitored by double-wavelength absorptiometry in the visible and ultraviolet ray regions.

  13. Quantitative Shape Measurements of Distal Volcanic Ash Colleen M. Riley, William I. Rose, and Gregg J.S. Bluth

    E-Print Network [OSTI]

    Rose, William I.

    Quantitative Shape Measurements of Distal Volcanic Ash Colleen M. Riley, William I. Rose, and Gregg-7093; Email: colleenandahi@hotmail.com #12;2 Abstract Large-scale volcanic eruptions produce fine ash ( distances from the volcanic source, thus, becoming a hazard to aircraft and public health. Ash particles

  14. A New Method for Quantitative Marking of Deposited Lithium via Chemical Treatment on Graphite Anodes in Lithium-Ion Cells

    E-Print Network [OSTI]

    Schmidt, Volker

    A New Method for Quantitative Marking of Deposited Lithium via Chemical Treatment on Graphite Anodes in Lithium-Ion Cells Yvonne Krämer*[a] , Claudia Birkenmaier[b] , Julian Feinauer[a,c] , Andreas*[e] and Thomas Schleid[f] Abstract: A novel approach for the marking of deposited lithium on graphite anodes from

  15. ROBUST CONTROL DESIGN OF A SINGLE DEGREE-OF-FREEDOM MAGNETIC LEVITATION SYSTEM BY QUANTITATIVE FEEDBACK THEORY

    E-Print Network [OSTI]

    Nagurka, Mark L.

    traditional mechanical parts. In high-speed maglev train systems and magnetic bearing systems, non parameter changes are common. Two examples of changes in the levitated load are: (1) a magnetic bearing expeROBUST CONTROL DESIGN OF A SINGLE DEGREE-OF-FREEDOM MAGNETIC LEVITATION SYSTEM BY QUANTITATIVE

  16. Cytokine detection in eiav-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction 

    E-Print Network [OSTI]

    Allen, Charlotte Annette

    2009-05-15

    . In the current studies a set of quantitative real-time polymerase chain reaction (QPCR) assays for the equine cytokines IL-1?, IL-1?, IL-6, IL-8 and TNF-? were validated using QPCR primers and probes which were generated for the aforementioned equine genes....

  17. Hartmut Durchschlag, Cargill Hartmut Durchschlag recently joined Cargill as a Senior Commodity Quantitative Analyst. Prior to that, Mr. Durchschlag

    E-Print Network [OSTI]

    Ciocan-Fontanine, Ionut

    Manage- ment and Modeling at ABN AMRO and a Senior Derivatives Specialist at Santander Investment Bank on trading, hedging and risk management strategies and programs for oil and gas companies. Nelson has also officer for UBS Investment Bank. He began his career in the quantitative research group at Enron

  18. 10. international mouse genome conference

    SciTech Connect (OSTI)

    Meisler, M.H.

    1996-12-31

    Ten years after hosting the First International Mammalian Genome Conference in Paris in 1986, Dr. Jean-Louis Guenet presided over the Tenth Conference at the Pasteur Institute, October 7--10, 1996. The 1986 conference was a satellite to the Human Gene Mapping Workshop and had approximately 50 attendees. The 1996 meeting was attended by 300 scientists from around the world. In the interim, the number of mapped loci in the mouse increased from 1,000 to over 20,000. This report contains a listing of the program and its participants, and two articles that review the meeting and the role of the laboratory mouse in the Human Genome project. More than 200 papers were presented at the conference covering the following topics: International mouse chromosome committee meetings; Mutant generation and identification; Physical and genetic maps; New technology and resources; Chromatin structure and gene regulation; Rate and hamster genetic maps; Informatics and databases; and Quantitative trait analysis.

  19. Quantitative studies of human cardiac metabolism by /sup 31/P rotating-frame NMR

    SciTech Connect (OSTI)

    Blackledge, M.J.; Rajagopalan, B.; Oberhaensli, R.D.; Bolas, N.M.; Styles, P.; Radda, G.K.

    1987-06-01

    The authors have developed /sup 31/P NMR spectroscopic methods to determine quantitatively relative levels of phosphorus-containing metabolites in the human myocardium. They have used localization techniques based on the rotating-frame imaging experiment and carried out the double-surface coil probe. Information is obtained from selected slices by rotating-frame depth selection and from a complete one-dimensional spectroscopic image using phase-modulated rotating-frame imaging. The methods collect biochemical information from metabolites in human heart, and they use the fact that the phosphocreatine/ATP molar ratio in skeletal muscle at rest is higher than that in working heart to demonstrate that localization ha been achieved for each investigation. The phosphocreatine/ATP molar ratio in normal human heart has been measured as 1.55 +/- 0.20 in six subjects using dept selection and as 1.53 +/- 0.25 in four subjects using spectroscopic imaging. Measurement of this ratio is expected to give a useful and reproducible index of myocardial energetics in normal and pathological states.

  20. Quantitative infrared absorption cross-sections of isoprene for atmospheric measurements

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Brauer, C. S.; Blake, T. A.; Guenther, A. B.; Sams, R. L.; Johnson, T. J.

    2014-04-25

    Isoprene (C5H8, 2-methyl-1,3-butadiene) is a volatile organic compound (VOC) that is one of the primary contributors to annual global VOC emissions. Produced by vegetation as well as anthropogenic sources, the OH- and O3-initiated oxidations of isoprene are a major source of atmospheric oxygenated organics. Few quantitative infrared studies have been reported for isoprene, however, limiting the ability to quantify isoprene emissions via stand-off infrared or in situ detection. We thus report absorption coefficients and integrated band intensities for isoprene in the 600–6500 cm?1 region. The pressure-broadened (1 atmosphere N2) spectra were recorded at 278, 298 and 323 K in amore »19.94 cm path length cell at 0.112 cm?1 resolution, using a Bruker 66v FTIR. Composite spectra are derived from a minimum of seven isoprene sample pressures at each temperature and the number densities are normalized to 296 K and 1 atmosphere.« less

  1. ANSI/ASHRAE/IESNA Standard 90.1-2007 Final Determination Quantitative Analysis

    SciTech Connect (OSTI)

    Halverson, Mark A.; Liu, Bing; Richman, Eric E.; Winiarski, David W.

    2011-05-01

    The United States (U.S.) Department of Energy (DOE) conducted a final quantitative analysis to assess whether buildings constructed according to the requirements of the American National Standards Institute (ANSI)/American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE)/Illuminating Engineering Society of North America (IESNA) Standard 90.1-2007 would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IESNA Standard 90.1-2004. The final analysis considered each of the 44 addenda to ANSI/ASHRAE/IESNA Standard 90.1-2004 that were included in ANSI/ASHRAE/IESNA Standard 90.1-2007. All 44 addenda processed by ASHRAE in the creation of Standard 90.1-2007 from Standard 90.1-2004 were reviewed by DOE, and their combined impact on a suite of 15 building prototype models in 15 ASHRAE climate zones was considered. Most addenda were deemed to have little quantifiable impact on building efficiency for the purpose of DOE’s final determination. However, out of the 44 addenda, 9 were preliminarily determined to have measureable and quantifiable impact.

  2. ANSI/ASHRAE/IESNA Standard 90.1-2010 Preliminary Determination Quantitative Analysis

    SciTech Connect (OSTI)

    Halverson, Mark A.; Liu, Bing; Rosenberg, Michael I.

    2010-11-01

    The United States (U.S.) Department of Energy (DOE) conducted a preliminary quantitative analysis to assess whether buildings constructed according to the requirements of the American National Standards Institute (ANSI)/American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE)/Illuminating Engineering Society of North America (IESNA) Standard 90.1-2010 (ASHRAE Standard 90.1-2010, Standard 90.1-2010, or 2010 edition) would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IESNA Standard 90.1-2007(ASHRAE Standard 90.1-2007, Standard 90.1-2007, or 2007 edition). The preliminary analysis considered each of the 109 addenda to ASHRAE Standard 90.1-2007 that were included in ASHRAE Standard 90.1-2010. All 109 addenda processed by ASHRAE in the creation of Standard 90.1-2010 from Standard 90.1-2007 were reviewed by DOE, and their combined impact on a suite of 16 building prototype models in 15 ASHRAE climate zones was considered. Most addenda were deemed to have little quantifiable impact on building efficiency for the purpose of DOE’s preliminary determination. However, out of the 109 addenda, 34 were preliminarily determined to have measureable and quantifiable impact.

  3. Quantitative interpretation of the transition voltages in gold-poly(phenylene) thiol-gold molecular junctions

    SciTech Connect (OSTI)

    Wu, Kunlin; Bai, Meilin; Hou, Shimin; Sanvito, Stefano

    2013-11-21

    The transition voltage of three different asymmetric Au/poly(phenylene) thiol/Au molecular junctions in which the central molecule is either benzene thiol, biphenyl thiol, or terphenyl thiol is investigated by first-principles quantum transport simulations. For all the junctions, the calculated transition voltage at positive polarity is in quantitative agreement with the experimental values and shows weak dependence on alterations of the Au-phenyl contact. When compared to the strong coupling at the Au-S contact, which dominates the alignment of various molecular orbitals with respect to the electrode Fermi level, the coupling at the Au-phenyl contact produces only a weak perturbation. Therefore, variations of the Au-phenyl contact can only have a minor influence on the transition voltage. These findings not only provide an explanation to the uniformity in the transition voltages found for ?-conjugated molecules measured with different experimental methods, but also demonstrate the advantage of transition voltage spectroscopy as a tool for determining the positions of molecular levels in molecular devices.

  4. Nucleophosmin in the pathogenesis of arsenic-related bladder carcinogenesis revealed by quantitative proteomics

    SciTech Connect (OSTI)

    Chen Shuhui [Department of Chemistry, College of Sciences, National Cheng Kung University, Tainan 701, Taiwan (China); Wang Yiwen [Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Hsu Jueliang [Department of Chemistry, College of Sciences, National Cheng Kung University, Tainan 701, Taiwan (China); Chang Hongyi [Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Wang Chiyun [Graduate Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Shen Potsun [Department of Chemistry, College of Sciences, National Cheng Kung University, Tainan 701, Taiwan (China); Chiang Chiwu [Graduate Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Chuang Jingjing [Department of Microbiology and Immunology, National Chiayi University, Chiayi 600, Taiwan (China); Tsai Hungwen [Department of Pathology, National Cheng Kung University Hospital, Tainan 704, Taiwan (China); Gu Powen [Department of Clinical Pathology, Chang Gung Memorial Hospital, Tao-Yuan 333, Taiwan (China); Chang Fangchih [Instrument Center, National Cheng Kung University, Tainan 701, Taiwan (China); Liu Hsiaosheng, E-mail: a713@mail.ncku.edu.t [Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Chow Nanhaw, E-mail: chownh@mail.ncku.edu.t [Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Graduate Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China); Department of Pathology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan (China)

    2010-01-15

    To investigate the molecular mechanisms of arsenic (As)-associated carcinogenesis, we performed proteomic analysis on E7 immortalized human uroepithelial cells after treatment with As in vitro. Quantitative proteomics was performed using stable isotope dimethyl labeling coupled with two-dimensional liquid chromatography peptide separation and mass spectrometry (MS)/MS analysis. Among 285 proteins, a total of 26 proteins were upregulated (ratio > 2.0) and 18 proteins were downregulated (ratio < 0.65) by As treatment, which are related to nucleotide binding, lipid metabolism, protein folding, protein biosynthesis, transcription, DNA repair, cell cycle control, and signal transduction. This study reports the potential significance of nucleophosmin (NPM) in the As-related bladder carcinogenesis. NPM was universally expressed in all of uroepithelial cell lines examined, implying that NPM may play a role in human bladder carcinogenesis. Upregulation of NPM tends to be dose- and time-dependent after As treatment. Expression of NPM was associated with cell proliferation, migration and anti-apoptosis. On the contrary, soy isoflavones inhibited the expression of NPM in vitro. The results suggest that NPM may play a role in the As-related bladder carcinogenesis, and soybean-based foods may have potential in the suppression of As/NPM-related tumorigenesis.

  5. Quantitative law describing market dynamics before and after interest-rate change

    SciTech Connect (OSTI)

    Petersen, Alexander M.; Wang Fengzhong; Stanley, H. Eugene; Havlin, Shlomo

    2010-06-15

    We study the behavior of U.S. markets both before and after U.S. Federal Open Market Commission meetings and show that the announcement of a U.S. Federal Reserve rate change causes a financial shock, where the dynamics after the announcement is described by an analog of the Omori earthquake law. We quantify the rate n(t) of aftershocks following an interest-rate change at time T and find power-law decay which scales as n(t-T)approx(t-T){sup -O}MEGA, with OMEGA positive. Surprisingly, we find that the same law describes the rate n{sup '}(|t-T|) of 'preshocks' before the interest-rate change at time T. This study quantitatively relates the size of the market response to the news which caused the shock and uncovers the presence of quantifiable preshocks. We demonstrate that the news associated with interest-rate change is responsible for causing both the anticipation before the announcement and the surprise after the announcement. We estimate the magnitude of financial news using the relative difference between the U.S. Treasury Bill and the Federal Funds effective rate. Our results are consistent with the 'sign effect', in which 'bad news' has a larger impact than 'good news'. Furthermore, we observe significant volatility aftershocks, confirming a 'market under-reaction' that lasts at least one trading day.

  6. Delineating Rearrangements in Single Yeast Artificial Chromosomes by Quantitative DNA Fiber Mapping

    SciTech Connect (OSTI)

    Weier, Heinz-Ulrich G.; Greulich-Bode, Karin M.; Wu, Jenny; Duell, Thomas

    2009-09-18

    Cloning of large chunks of human genomic DNA in recombinant systems such as yeast or bacterial artificial chromosomes has greatly facilitated the construction of physical maps, the positional cloning of disease genes or the preparation of patient-specific DNA probes for diagnostic purposes. For this process to work efficiently, the DNA cloning process and subsequent clone propagation need to maintain stable inserts that are neither deleted nor otherwise rearranged. Some regions of the human genome; however, appear to have a higher propensity than others to rearrange in any host system. Thus, techniques to detect and accurately characterize such rearrangements need to be developed. We developed a technique termed 'Quantitative DNA Fiber Mapping (QDFM)' that allows accurate tagging of sequence elements of interest with near kilobase accuracy and optimized it for delineation of rearrangements in recombinant DNA clones. This paper demonstrates the power of this microscopic approach by investigating YAC rearrangements. In our examples, high-resolution physical maps for regions within the immunoglobulin lambda variant gene cluster were constructed for three different YAC clones carrying deletions of 95 kb and more. Rearrangements within YACs could be demonstrated unambiguously by pairwise mapping of cosmids along YAC DNA molecules. When coverage by YAC clones was not available, distances between cosmid clones were estimated by hybridization of cosmids onto DNA fibers prepared from human genomic DNA. In addition, the QDFM technology provides essential information about clone stability facilitating closure of the maps of the human genome as well as those of model organisms.

  7. Highly Quantitative Electrochemical Characterization of Non-Aqueous Electrolytes & Solid Electrolyte Interphases

    SciTech Connect (OSTI)

    Sergiy V. Sazhin; Kevin L. Gering; Mason K. Harrup; Harry W. Rollins

    2012-10-01

    The methods to measure solid electrolyte interphase (SEI) electrochemical properties and SEI formation capability of non-aqueous electrolyte solutions are not adequately addressed in the literature. And yet, there is a strong demand in new electrolyte generations that promote stabilized SEIs and have an influence to resolve safety, calendar life and other limitations of Li-ion batteries. To fill this gap, in situ electrochemical approach with new descriptive criteria for highly quantitative characterization of SEI and electrolytes is proposed. These criteria are: SEI formation capacity, SEI corrosion rate, SEI maintenance rate, and SEI kinetic stability. These criteria are associated with battery parameters like irreversible capacity, self-discharge, shelf-life, power, etc. Therefore, they are especially useful for electrolyte development and standard fast screening, allowing a skillful approach to narrow down the search for the best electrolyte. The characterization protocol also allows retrieving information on interfacial resistance for SEI layers and the electrochemical window of electrolytes, the other important metrics of characterization. The method validation was done on electrolyte blends containing phosphazenes, developed at Idaho National Laboratory, as 1.2M LiPF6 [80 % EC-MEC (2:8) (v/v) + 20% Phosphazene variety] (v/v), which were targeted for safer electrolyte variations.

  8. The Power of a Good Idea: Quantitative Modeling of the Spread of Ideas from Epidemiological Models

    SciTech Connect (OSTI)

    Bettencourt, L. M. A. (LANL); Cintron-Arias, A. (Cornell University); Kaiser, D. I. (MIT); Castillo-Chavez, C. (Arizona State University)

    2005-05-05

    The population dynamics underlying the diffusion of ideas hold many qualitative similarities to those involved in the spread of infections. In spite of much suggestive evidence this analogy is hardly ever quantified in useful ways. The standard benefit of modeling epidemics is the ability to estimate quantitatively population average parameters, such as interpersonal contact rates, incubation times, duration of infectious periods, etc. In most cases such quantities generalize naturally to the spread of ideas and provide a simple means of quantifying sociological and behavioral patterns. Here we apply several paradigmatic models of epidemics to empirical data on the advent and spread of Feynman diagrams through the theoretical physics communities of the USA, Japan, and the USSR in the period immediately after World War II. This test case has the advantage of having been studied historically in great detail, which allows validation of our results. We estimate the effectiveness of adoption of the idea in the three communities and find values for parameters reflecting both intentional social organization and long lifetimes for the idea. These features are probably general characteristics of the spread of ideas, but not of common epidemics.

  9. Quantitative method for measuring heat flux emitted from a cryogenic object

    DOE Patents [OSTI]

    Duncan, R.V.

    1993-03-16

    The present invention is a quantitative method for measuring the total heat flux, and of deriving the total power dissipation, of a heat-fluxing object which includes the steps of placing an electrical noise-emitting heat-fluxing object in a liquid helium bath and measuring the superfluid transition temperature of the bath. The temperature of the liquid helium bath is thereafter reduced until some measurable parameter, such as the electrical noise, exhibited by the heat-fluxing object or a temperature-dependent resistive thin film in intimate contact with the heat-fluxing object, becomes greatly reduced. The temperature of the liquid helum bath is measured at this point. The difference between the superfluid transition temperature of the liquid helium bath surrounding the heat-fluxing object, and the temperature of the liquid helium bath when the electrical noise emitted by the heat-fluxing object becomes greatly reduced, is determined. The total heat flux from the heat-fluxing object is determined as a function of this difference between these temperatures. In certain applications, the technique can be used to optimize thermal design parameters of cryogenic electronics, for example, Josephson junction and infrared sensing devices.

  10. Quantitative maps of groundwater resources in Africa This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Jones, Peter JS

    Quantitative maps of groundwater resources in Africa This article has been downloaded from.1088/1748-9326/7/2/024009 Quantitative maps of groundwater resources in Africa A M MacDonald1, H C Bonsor1, B ´E ´O Dochartaigh1 and R G/024009 Abstract In Africa, groundwater is the major source of drinking water and its use for irrigation

  11. Exposure to monocrotophos pesticide during sexual development causes the feminization/demasculinization of the reproductive traits and a reduction in the reproductive success of male guppies (Poecilia reticulata)

    SciTech Connect (OSTI)

    Tian, Hua; Li, Yun; Wang, Wei; Wu, Peng; Ru, Shaoguo, E-mail: rusg@ouc.edu.cn

    2012-09-01

    Monocrotophos is a highly toxic organophosphorus pesticide that has been confirmed to be an endocrine?disrupting chemical. To evaluate the influence of this pollutant on the reproductive system of male fish, we studied the sex steroid levels, reproductive traits, sex ratio, and reproductive success in male guppies (Poecilia reticulata) exposed to 40% monocrotophos pesticide at the nominal concentrations of 0.01, 0.10, and 1.00 mg/L for 90 days from birth to adulthood in a semi?static exposure system. Radioimmunoassay and western blot analyses demonstrated that the long?term exposure to monocrotophos pesticide during the sexual development of male guppies caused a significant increase in 17??estradiol levels and consequently induced vitellogenin synthesis, suggesting the feminization of the males. Monocrotophos pesticide also caused a significant decrease in testosterone levels, which consequently inhibited testis growth and reduced the sperm count and the area and intensity of their sexually attractive orange spots, which collectively indicated the significant demasculinization of the male sexual characteristics. Furthermore, these changes in the sexual characteristics at the cellular and organ levels translated into ecologically important effects on the reproductive success at the individual level, as measured by a decrease in offspring production and survival rate. The present study provides the first evidence that monocrotophos pesticide can cause severe reproductive abnormalities in fish due to its endocrine?disrupting action. -- Highlights: ? Monocrotophos pesticide caused an increase in 17??estradiol levels of male guppies. ? Monocrotophos pesticide induced vitellogenin synthesis of male guppies. ? Monocrotophos pesticide caused a decrease in testosterone levels of male guppies. ? Monocrotophos pesticide caused demasculinization of male sexual characteristics. ? Monocrotophos pesticide caused a reduction in reproductive success of male guppies.

  12. ANSI/ASHRAE/IES Standard 90.1-2010 Final Determination Quantitative Analysis

    SciTech Connect (OSTI)

    Halverson, Mark A.; Rosenberg, Michael I.; Liu, Bing

    2011-10-31

    The U.S. Department of Energy (DOE) conducted a final quantitative analysis to assess whether buildings constructed according to the requirements of the American National Standards Institute (ANSI)/American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE)/Illuminating Engineering Society of North America (IESNA) Standard 90.1-2010 (ASHRAE Standard 90.1-2010, Standard 90.1-2010, or 2010 edition) would result in energy savings compared with buildings constructed to ANSI/ASHRAE/IESNA Standard 90.1-2007(ASHRAE Standard 90.1-2007, Standard 90.1-2007, or 2007 edition). The final analysis considered each of the 109 addenda to ASHRAE Standard 90.1-2007 that were included in ASHRAE Standard 90.1-2010. All 109 addenda processed by ASHRAE in the creation of Standard 90.1-2010 from Standard 90.1-2007 were reviewed by DOE, and their combined impact on a suite of 16 building prototype models in 15 ASHRAE climate zones was considered. Most addenda were deemed to have little quantifiable impact on building efficiency for the purpose of DOE's final determination. However, out of the 109 addenda, 34 were preliminarily determined to have a measureable and quantifiable impact. A suite of 240 computer energy simulations for building prototypes complying with ASHRAE 90.1-2007 was developed. These prototypes were then modified in accordance with these 34 addenda to create a second suite of corresponding building simulations reflecting the same buildings compliant with Standard 90.1-2010. The building simulations were conducted using the DOE EnergyPlus building simulation software. The resulting energy use from the complete suite of 480 simulation runs was then converted to energy use intensity (EUI, or energy use per unit floor area) metrics (Site EUI, Primary EUI, and energy cost intensity [ECI]) results for each simulation. For each edition of the standard, these EUIs were then aggregated to a national basis for each prototype using weighting factors based on construction floor area developed for each of the 15 U.S. climate zones using commercial construction data. When compared, the resulting weighted EUIs indicated that each of the 16 building prototypes used less energy under Standard 90.1-2010 than under Standard 90.1-2007 on a national basis when considering site energy, primary energy, or energy cost. The EUIs were also aggregated across building types to a national commercial building basis using the same weighting data. On a national basis, the final quantitative analysis estimated a floor-space-weighted national average reduction in new building energy consumption of 18.2 percent for source energy and 18.5 percent when considering site energy. An 18.2 percent savings in energy cost, based on national average commercial energy costs for electricity and natural gas, was also estimated.

  13. Quantitative Effects of Vehicle Parameters on Fuel Consumption for Heavy-Duty Vehicle

    SciTech Connect (OSTI)

    Wang, Lijuan; Kelly, Kenneth; Walkowicz, Kevin; Duran, Adam

    2015-10-16

    The National Renewable Energy Laboratory's (NREL's) Fleet Test and Evaluations team recently conducted chassis dynamometer tests of a class 8 conventional regional delivery truck over the Heavy Heavy-Duty Diesel Truck (HHDDT), West Virginia University City (WVU City), and Composite International Truck Local and Commuter Cycle (CILCC) drive cycles. A quantitative study was conducted by analyzing the impacts of various factors on fuel consumption (FC) and fuel economy (FE) by modeling and simulating the truck using NREL's Future Automotive Systems Technology Simulator (FASTSim). Factors used in this study included vehicle weight, and the coefficients of rolling resistance and aerodynamic drag. The simulation results from a single parametric study revealed that FC was approximately a linear function of the weight, coefficient of aerodynamic drag, and rolling resistance over various drive cycles. Among these parameters, the truck weight had the largest effect on FC. The study of the impact of two technologies on FE suggested that, depending on the circumstances, it may be more cost effective to reduce one parameter (such as coefficient of aerodynamic drag) to increase fuel economy, or it may be more beneficial to reduce another (such as the coefficient of rolling resistance). It also provided a convenient way to estimate FE by interpolating within the parameter values and extrapolating outside of them. The simulation results indicated that the FC could be reduced from 38.70 L/100 km, 50.72 L/100 km, and 38.42 L/100 km in the baseline truck to 26.78 L/100 km, 43.14 L/100 km and 29.84 L/100 km over the HHDDT, WVU City and CILCC drive cycles, respectively, when the U.S. Department of Energy's three targeted new technologies were applied simultaneously.

  14. Correction for FDG PET dose extravasations: Monte Carlo validation and quantitative evaluation of patient studies

    SciTech Connect (OSTI)

    Silva-Rodríguez, Jesús Aguiar, Pablo; Servicio de Medicina Nuclear, Complexo Hospitalario Universidade de Santiago de Compostela , 15782, Galicia; Grupo de Imaxe Molecular, Instituto de Investigación Sanitarias , Santiago de Compostela, 15706, Galicia ; Sánchez, Manuel; Mosquera, Javier; Luna-Vega, Víctor; Cortés, Julia; Garrido, Miguel; Pombar, Miguel; Ruibal, Álvaro; Grupo de Imaxe Molecular, Instituto de Investigación Sanitarias , Santiago de Compostela, 15706, Galicia; Fundación Tejerina, 28003, Madrid

    2014-05-15

    Purpose: Current procedure guidelines for whole body [18F]fluoro-2-deoxy-D-glucose (FDG)-positron emission tomography (PET) state that studies with visible dose extravasations should be rejected for quantification protocols. Our work is focused on the development and validation of methods for estimating extravasated doses in order to correct standard uptake value (SUV) values for this effect in clinical routine. Methods: One thousand three hundred sixty-seven consecutive whole body FDG-PET studies were visually inspected looking for extravasation cases. Two methods for estimating the extravasated dose were proposed and validated in different scenarios using Monte Carlo simulations. All visible extravasations were retrospectively evaluated using a manual ROI based method. In addition, the 50 patients with higher extravasated doses were also evaluated using a threshold-based method. Results: Simulation studies showed that the proposed methods for estimating extravasated doses allow us to compensate the impact of extravasations on SUV values with an error below 5%. The quantitative evaluation of patient studies revealed that paravenous injection is a relatively frequent effect (18%) with a small fraction of patients presenting considerable extravasations ranging from 1% to a maximum of 22% of the injected dose. A criterion based on the extravasated volume and maximum concentration was established in order to identify this fraction of patients that might be corrected for paravenous injection effect. Conclusions: The authors propose the use of a manual ROI based method for estimating the effectively administered FDG dose and then correct SUV quantification in those patients fulfilling the proposed criterion.

  15. A quantitative quantum-chemical analysis tool for the distribution of mechanical force in molecules

    SciTech Connect (OSTI)

    Stauch, Tim; Dreuw, Andreas

    2014-04-07

    The promising field of mechanochemistry suffers from a general lack of understanding of the distribution and propagation of force in a stretched molecule, which limits its applicability up to the present day. In this article, we introduce the JEDI (Judgement of Energy DIstribution) analysis, which is the first quantum chemical method that provides a quantitative understanding of the distribution of mechanical stress energy among all degrees of freedom in a molecule. The method is carried out on the basis of static or dynamic calculations under the influence of an external force and makes use of a Hessian matrix in redundant internal coordinates (bond lengths, bond angles, and dihedral angles), so that all relevant degrees of freedom of a molecule are included and mechanochemical processes can be interpreted in a chemically intuitive way. The JEDI method is characterized by its modest computational effort, with the calculation of the Hessian being the rate-determining step, and delivers, except for the harmonic approximation, exact ab initio results. We apply the JEDI analysis to several example molecules in both static quantum chemical calculations and Born-Oppenheimer Molecular Dynamics simulations in which molecules are subject to an external force, thus studying not only the distribution and the propagation of strain in mechanically deformed systems, but also gaining valuable insights into the mechanochemically induced isomerization of trans-3,4-dimethylcyclobutene to trans,trans-2,4-hexadiene. The JEDI analysis can potentially be used in the discussion of sonochemical reactions, molecular motors, mechanophores, and photoswitches as well as in the development of molecular force probes.

  16. Quantitative patterns of large-scale field-aligned currents in the auroral ionosphere

    SciTech Connect (OSTI)

    Foster, J.C.; Fuller-Rowell, T.; Evans, D.S.

    1989-03-01

    Quantitative patterns of the distribution of field-aligned current (FAC) density have been derived from gradients of the average patterns of the Hall and Pedersen currents at high latitudes under the assumption that the total current is divergence-free. The horizontal currents were calculated from empirical convection electric field models, derived from Millstone Hill radar observations, and the ionospheric Hall and Pedersen conductances, based on satellite observations of the precipitating particle energy flux and spectrum and including an average (equinox) solar contribution. These independent empirical models, and the resultant patterns of the field-aligned currents, are keyed to an auroral precipitation index which quantifies the intensity and spatial extent of high-latitude particle precipitation and which is determined from a single satellite crossing of the auroral precipitation pattern. The patterns detail the spatial distribution of the currents as a function of increasing disturbance level. The magnitudes of the total single-hemisphere currents into or out of the ionosphere are closely balanced at each activity level and increase exponentially between 0.1 and 6 MA with increasing values of the precipitation index. The interplanetary magnetic field (IMF) sector dependence of the FAC patterns is investigated for disturbed conditions. A large portion of the FAC pattern is closed by local Pedersen currents (current into the ionosphere is balanced by an equal current out of the ionosphere at that local time). This locally balanced portion of the FAC system is enhanced in the prenoon (postnoon) sector for IMF B/sub v/>+1 nT (B/sub y/<-1 nT). In addition, there are net currents into the ionosphere postnoon and out of the ionosphere in the premidnight sector.

  17. The Strategic Environment Assessment bibliographic network: A quantitative literature review analysis

    SciTech Connect (OSTI)

    Caschili, Simone, E-mail: s.caschili@ucl.ac.uk [UCL QASER Lab, University College London, Gower Street, London WC1E 6BT (United Kingdom); De Montis, Andrea; Ganciu, Amedeo; Ledda, Antonio; Barra, Mario [Dipartimento di Agraria, University of Sassari, viale Italia, 39, 07100 Sassari (Italy)

    2014-07-01

    Academic literature has been continuously growing at such a pace that it can be difficult to follow the progression of scientific achievements; hence, the need to dispose of quantitative knowledge support systems to analyze the literature of a subject. In this article we utilize network analysis tools to build a literature review of scientific documents published in the multidisciplinary field of Strategic Environment Assessment (SEA). The proposed approach helps researchers to build unbiased and comprehensive literature reviews. We collect information on 7662 SEA publications and build the SEA Bibliographic Network (SEABN) employing the basic idea that two publications are interconnected if one cites the other. We apply network analysis at macroscopic (network architecture), mesoscopic (sub graph) and microscopic levels (node) in order to i) verify what network structure characterizes the SEA literature, ii) identify the authors, disciplines and journals that are contributing to the international discussion on SEA, and iii) scrutinize the most cited and important publications in the field. Results show that the SEA is a multidisciplinary subject; the SEABN belongs to the class of real small world networks with a dominance of publications in Environmental studies over a total of 12 scientific sectors. Christopher Wood, Olivia Bina, Matthew Cashmore, and Andrew Jordan are found to be the leading authors while Environmental Impact Assessment Review is by far the scientific journal with the highest number of publications in SEA studies. - Highlights: • We utilize network analysis to analyze scientific documents in the SEA field. • We build the SEA Bibliographic Network (SEABN) of 7662 publications. • We apply network analysis at macroscopic, mesoscopic and microscopic network levels. • We identify SEABN architecture, relevant publications, authors, subjects and journals.

  18. A quantitative acoustic emission study on fracture processes in ceramics based on wavelet packet decomposition

    SciTech Connect (OSTI)

    Ning, J. G.; Chu, L.; Ren, H. L.

    2014-08-28

    We base a quantitative acoustic emission (AE) study on fracture processes in alumina ceramics on wavelet packet decomposition and AE source location. According to the frequency characteristics, as well as energy and ringdown counts of AE, the fracture process is divided into four stages: crack closure, nucleation, development, and critical failure. Each of the AE signals is decomposed by a 2-level wavelet package decomposition into four different (from-low-to-high) frequency bands (AA{sub 2}, AD{sub 2}, DA{sub 2}, and DD{sub 2}). The energy eigenvalues P{sub 0}, P{sub 1}, P{sub 2}, and P{sub 3} corresponding to these four frequency bands are calculated. By analyzing changes in P{sub 0} and P{sub 3} in the four stages, we determine the inverse relationship between AE frequency and the crack source size during ceramic fracture. AE signals with regard to crack nucleation can be expressed when P{sub 0} is less than 5 and P{sub 3} more than 60; whereas AE signals with regard to dangerous crack propagation can be expressed when more than 92% of P{sub 0} is greater than 4, and more than 95% of P{sub 3} is less than 45. Geiger location algorithm is used to locate AE sources and cracks in the sample. The results of this location algorithm are consistent with the positions of fractures in the sample when observed under a scanning electronic microscope; thus the locations of fractures located with Geiger's method can reflect the fracture process. The stage division by location results is in a good agreement with the division based on AE frequency characteristics. We find that both wavelet package decomposition and Geiger's AE source locations are suitable for the identification of the evolutionary process of cracks in alumina ceramics.

  19. THE SAP3 COMPUTER PROGRAM FOR QUANTITATIVE MULTIELEMENT ANALYSIS BY ENERGY DISPERSIVE X-RAY FLUORESCENCE

    SciTech Connect (OSTI)

    Nielson, K. K.; Sanders, R. W.

    1982-04-01

    SAP3 is a dual-function FORTRAN computer program which performs peak analysis of energy-dispersive x-ray fluorescence spectra and then quantitatively interprets the results of the multielement analysis. It was written for mono- or bi-chromatic excitation as from an isotopic or secondary excitation source, and uses the separate incoherent and coherent backscatter intensities to define the bulk sample matrix composition. This composition is used in performing fundamental-parameter matrix corrections for self-absorption, enhancement, and particle-size effects, obviating the need for specific calibrations for a given sample matrix. The generalized calibration is based on a set of thin-film sensitivities, which are stored in a library disk file and used for all sample matrices and thicknesses. Peak overlap factors are also determined from the thin-film standards, and are stored in the library for calculating peak overlap corrections. A detailed description is given of the algorithms and program logic, and the program listing and flow charts are also provided. An auxiliary program, SPCAL, is also given for use in calibrating the backscatter intensities. SAP3 provides numerous analysis options via seventeen control switches which give flexibility in performing the calculations best suited to the sample and the user needs. User input may be limited to the name of the library, the analysis livetime, and the spectrum filename and location. Output includes all peak analysis information, matrix correction factors, and element concentrations, uncertainties and detection limits. Twenty-four elements are typically determined from a 1024-channel spectrum in one-to-two minutes using a PDP-11/34 computer operating under RSX-11M.

  20. A quantitative approach to the characterization of cumulative and average solvent exposure in paint manufacturing plants

    SciTech Connect (OSTI)

    Ford, D.P.; Schwartz, B.S.; Powell, S.; Nelson, T.; Keller, L.; Sides, S.; Agnew, J.; Bolla, K.; Bleecker, M. )

    1991-06-01

    Previous reports have attributed a range of neurobehavioral effects to low-level, occupational solvent exposure. These studies have generally been limited in their exposure assessments and have specifically lacked good estimates of exposure intensity. In the present study, the authors describe the development of two exposure variables that quantitatively integrate industrial hygiene sampling data with estimates of exposure duration--a cumulative exposure (CE) estimate and a lifetime weighted average exposure (LWAE) estimate. Detailed occupational histories were obtained from 187 workers at two paint manufacturing plants. Historic industrial hygiene sampling data for total hydrocarbons (a composite variable of the major neurotoxic solvents present) were grouped according to 20 uniform, temporally stable exposure zones, which had been defined during plant walk-through surveys. Sampling at the time of the study was used to characterize the few zones for which historic data were limited or unavailable. For each participant, the geometric mean total hydrocarbon level for each exposure zone worked in was multiplied by the duration of employment in that zone; the resulting products were summed over the working lifetime to create the CE variable. The CE variable was divided by the total duration of employment in solvent-exposed jobs to create the LWAE variable. The explanatory value of each participant's LWAE estimate in the regression of simple visual reaction time (a neurobehavioral test previously shown to be affected by chronic solvent exposure) on exposure was compared with that of several other exposure variables, including exposure duration and an exposure variable based on an ordinal ranking of the exposure zones.

  1. Mammographic quantitative image analysis and biologic image composition for breast lesion characterization and classification

    SciTech Connect (OSTI)

    Drukker, Karen Giger, Maryellen L.; Li, Hui; Duewer, Fred; Malkov, Serghei; Joe, Bonnie; Kerlikowske, Karla; Shepherd, John A.; Flowers, Chris I.; Drukteinis, Jennifer S.

    2014-03-15

    Purpose: To investigate whether biologic image composition of mammographic lesions can improve upon existing mammographic quantitative image analysis (QIA) in estimating the probability of malignancy. Methods: The study population consisted of 45 breast lesions imaged with dual-energy mammography prior to breast biopsy with final diagnosis resulting in 10 invasive ductal carcinomas, 5 ductal carcinomain situ, 11 fibroadenomas, and 19 other benign diagnoses. Analysis was threefold: (1) The raw low-energy mammographic images were analyzed with an established in-house QIA method, “QIA alone,” (2) the three-compartment breast (3CB) composition measure—derived from the dual-energy mammography—of water, lipid, and protein thickness were assessed, “3CB alone”, and (3) information from QIA and 3CB was combined, “QIA + 3CB.” Analysis was initiated from radiologist-indicated lesion centers and was otherwise fully automated. Steps of the QIA and 3CB methods were lesion segmentation, characterization, and subsequent classification for malignancy in leave-one-case-out cross-validation. Performance assessment included box plots, Bland–Altman plots, and Receiver Operating Characteristic (ROC) analysis. Results: The area under the ROC curve (AUC) for distinguishing between benign and malignant lesions (invasive and DCIS) was 0.81 (standard error 0.07) for the “QIA alone” method, 0.72 (0.07) for “3CB alone” method, and 0.86 (0.04) for “QIA+3CB” combined. The difference in AUC was 0.043 between “QIA + 3CB” and “QIA alone” but failed to reach statistical significance (95% confidence interval [–0.17 to + 0.26]). Conclusions: In this pilot study analyzing the new 3CB imaging modality, knowledge of the composition of breast lesions and their periphery appeared additive in combination with existing mammographic QIA methods for the distinction between different benign and malignant lesion types.

  2. Foundations for quantitative microstructural models to track evolution of the metallurgical state during high purity Nb cavity fabrication

    SciTech Connect (OSTI)

    Bieler, Thomas R; Wright, Neil T; Compton, Chris C

    2014-03-15

    The goal of the Materials Science SRF Cavity Group of Michigan State University and the National Superconducting Cyclotron has been (and continues to be) to understand quantitatively the effects of process history on functional properties. These relationships were assessed via studies on Nb samples and cavity parts, which had various combinations of forming processes, welding, heat treatments, and surface preparation. A primary focus was on large-grain cavity building strategies. Effects of processing operations and exposure to hydrogen on the thermal conductivity has been identified in single and bi-crystal samples, showing that the thermal conductivity can be altered by a factor of 5 depending on process history. Characterization of single crystal tensile samples show a strong effect of crystal orientation on deformation resistance and shape changes. Large grain half cells were examined to characterize defect content and surface damage effects, which provided quantitative information about the depth damage layers from forming.

  3. Formation resistivity measurements from within a cased well used to quantitatively determine the amount of oil and gas present

    DOE Patents [OSTI]

    Vail, III, William Banning (Bothell, WA)

    2000-01-01

    Methods to quantitatively determine the separate amounts of oil and gas in a geological formation adjacent to a cased well using measurements of formation resistivity. The steps include obtaining resistivity measurements from within a cased well of a given formation, obtaining the porosity, obtaining the resistivity of formation water present, computing the combined amounts of oil and gas present using Archie's Equations, determining the relative amounts of oil and gas present from measurements within a cased well, and then quantitatively determining the separate amounts of oil and gas present in the formation. Resistivity measurements are obtained from within the cased well by conducting A.C. current from within the cased well to a remote electrode at a frequency that is within the frequency range of 0.1 Hz to 20 Hz.

  4. Quantitative Analysis of Variability and Uncertainty in Environmental Data and Models. Volume 1. Theory and Methodology Based Upon Bootstrap Simulation

    SciTech Connect (OSTI)

    Frey, H. Christopher; Rhodes, David S.

    1999-04-30

    This is Volume 1 of a two-volume set of reports describing work conducted at North Carolina State University sponsored by Grant Number DE-FG05-95ER30250 by the U.S. Department of Energy. The title of the project is “Quantitative Analysis of Variability and Uncertainty in Acid Rain Assessments.” The work conducted under sponsorship of this grant pertains primarily to two main topics: (1) development of new methods for quantitative analysis of variability and uncertainty applicable to any type of model; and (2) analysis of variability and uncertainty in the performance, emissions, and cost of electric power plant combustion-based NOx control technologies. These two main topics are reported separately in Volumes 1 and 2.

  5. Spatial and Quantitative Approach to Incorporating Stakeholder Values into Total Maximum Daily Loads: Dominguez Channel Case Study

    SciTech Connect (OSTI)

    Stewart, J S; Baginski, T A; Greene, K G; Smith, A; Sicherman, A

    2006-06-23

    The Federal Clean Water Act (CWA) Section 303(d)(1)(A) requires each state to identify those waters that are not achieving water quality standards. The result of this assessment is called the 303(d) list. The CWA also requires states to develop and implement Total Maximum Daily Loads (TMDLs) for these waters on the 303(d) list. A TMDL specifies the maximum amount of a pollutant that a water body can receive and still meet water quality standards, and allocates the pollutant loadings to point and non-point sources. Nationwide, over 34,900 segments of waterways have been listed as impaired by the Environmental Protection Agency (EPA 2006). The EPA enlists state agencies and local communities to submit TMDL plans to reduce discharges by specified dates or have them developed by the EPA. The Department of Energy requested Lawrence Livermore National Laboratory (LLNL) to develop appropriate tools to assist in improving the TMDL process. An investigation of this process by LLNL found that plans to reduce discharges were being developed based on a wide range of site investigation methods. Our investigation found that given the resources available to the interested and responsible parties, developing a quantitative stakeholder input process and using visualization tools to display quantitative information could improve the acceptability of TMDL plans. We developed a stakeholder allocation model (SAM) which uses multi-attribute utility theory to quantitatively structure the preferences of the major stakeholder groups. We then applied GIS to display allocation options in maps representing economic activity, community groups, and city agencies. This allows allocation options and stakeholder concerns to be represented in both space and time. The primary goal of this tool is to provide a quantitative and visual display of stakeholder concerns over possible TMDL options.

  6. Autism traits in the RASopathies

    E-Print Network [OSTI]

    2013-01-01

    in Prevalence of Parent-reported Autism Spectrum Disorder inReports 2013. Baio J. Prevalence of autism spectrumdisorders—autism and developmental disabilities monitoring

  7. Autism traits in the RASopathies

    E-Print Network [OSTI]

    2013-01-01

    Dev Disord R: A language and environment for statisticalin R (R: A language and environment for statistical comput-

  8. Quantitative Assessment of Detection Frequency for the INL Ambient Air Monitoring Network

    SciTech Connect (OSTI)

    A. Jeffrey Sondrup; Arthur S. Rood

    2014-11-01

    A quantitative assessment of the Idaho National Laboratory (INL) air monitoring network was performed using frequency of detection as the performance metric. The INL air monitoring network consists of 37 low-volume air samplers in 31 different locations. Twenty of the samplers are located on INL (onsite) and 17 are located off INL (offsite). Detection frequencies were calculated using both BEA and ESER laboratory minimum detectable activity (MDA) levels. The CALPUFF Lagrangian puff dispersion model, coupled with 1 year of meteorological data, was used to calculate time-integrated concentrations at sampler locations for a 1-hour release of unit activity (1 Ci) for every hour of the year. The unit-activity time-integrated concentration (TICu) values were calculated at all samplers for releases from eight INL facilities. The TICu values were then scaled and integrated for a given release quantity and release duration. All facilities modeled a ground-level release emanating either from the center of the facility or at a point where significant emissions are possible. In addition to ground-level releases, three existing stacks at the Advanced Test Reactor Complex, Idaho Nuclear Technology and Engineering Center, and Material and Fuels Complex were also modeled. Meteorological data from the 35 stations comprising the INL Mesonet network, data from the Idaho Falls Regional airport, upper air data from the Boise airport, and three-dimensional gridded data from the weather research forecasting model were used for modeling. Three representative radionuclides identified as key radionuclides in INL’s annual National Emission Standards for Hazardous Air Pollutants evaluations were considered for the frequency of detection analysis: Cs-137 (beta-gamma emitter), Pu-239 (alpha emitter), and Sr-90 (beta emitter). Source-specific release quantities were calculated for each radionuclide, such that the maximum inhalation dose at any publicly accessible sampler or the National Emission Standards for Hazardous Air Pollutants maximum exposed individual location (i.e., Frenchman’s Cabin) was no more than 0.1 mrem yr–1 (i.e., 1% of the 10 mrem yr–1 standard). Detection frequencies were calculated separately for the onsite and offsite monitoring network. As expected, detection frequencies were generally less for the offsite sampling network compared to the onsite network. Overall, the monitoring network is very effective at detecting the potential releases of Cs-137 or Sr-90 from all sources/facilities using either the ESER or BEA MDAs. The network was less effective at detecting releases of Pu-239. Maximum detection frequencies for Pu-239 using ESER MDAs ranged from 27.4 to 100% for onsite samplers and 3 to 80% for offsite samplers. Using BEA MDAs, the maximum detection frequencies for Pu-239 ranged from 2.1 to 100% for onsite samplers and 0 to 5.9% for offsite samplers. The only release that was not detected by any of the samplers under any conditions was a release of Pu-239 from the Idaho Nuclear Technology and Engineering Center main stack (CPP-708). The methodology described in this report could be used to improve sampler placement and detection frequency, provided clear performance objectives are defined.

  9. Stress tensor: A quantitative indicator of effective volume and stability of helium in metals This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Simons, Jack

    Stress tensor: A quantitative indicator of effective volume and stability of helium in metals.1209/0295-5075/96/66001 Stress tensor: A quantitative indicator of effective volume and stability of helium in metals H.-B. Zhou1 demonstrate a quantitative stress indicator as well as a qualitative hard-sphere lattice model to characterize

  10. Development of mass spectrometry based technologies for quantitative cell signaling phosphoproteomics : the epidermal growth factor receptor family as a model system

    E-Print Network [OSTI]

    Wolf Yadlin, Alejandro

    2007-01-01

    Ligand binding to cell surface receptors initiates a cascade of signaling events regulated by dynamic phosphorylation on a multitude of pathway proteins. Quantitative features, including intensity, timing, and duration of ...

  11. Parsing ERK Activation Reveals Quantitatively Equivalent Contributions From Epidermal Growth Factor Receptor and HER2 In Human Mammary Epithelial Cells

    SciTech Connect (OSTI)

    Hendriks, Bart S.; Orr, Galya; Wells, Alan H.; Wiley, H. S.; Lauffenburger, Douglas A.

    2005-02-18

    HER2, a member of the EGFR tyrosine kinase family, functions as an accessory EGFR signaling component and alters EGFR trafficking by heterodimerization. HER2 overexpression leads to aberrant cell behavior including enhanced proliferation and motility. Here we apply a combination of computational modeling and quantitative experimental studies of the dynamic interactions between EGFR and HER2, and their downstream activation of extracellular signal-related kinase (ERK) to understand this complex signaling system. Using cells expressing different levels of HER2 relative to the EGFR, we can separate relative contributions of EGFR and HER2 to signaling amplitude and duration. Based on our model calculations, we demonstrate that, in contrast with previous suggestions in the literature, the intrinsic capabilities of EGFR and HER2 to activated ERK are quantitatively equivalent . We find that HER2-mediated effects on EGFR dimerization and trafficking are sufficient to explain the detected HER2-mediated amplification of EGF-induced ERK signaling. Our model suggests that transient amplification of ERK activity by HER2 arises predominantly from the 2-to-1 stoichiometry of receptor kinase to bound ligand in EGFR/HER2 heterodimers compared to the 1-to-1 stoichiometry of the EGFR homodimer, but alterations in receptor trafficking, with resultant EGFR sparing, cause the sustained HER2-mediated enhancement of ERK signaling.

  12. Assessment of ERCC1 and XPF Protein Expression Using Quantitative Immunohistochemistry in Nasopharyngeal Carcinoma Patients Undergoing Curative Intent Treatment

    SciTech Connect (OSTI)

    Jagdis, Amanda; Phan, Tien; Faculty of Medicine, University of Calgary, Calgary, Alberta ; Klimowicz, Alexander C.; Faculty of Medicine, University of Calgary, Calgary, Alberta ; Laskin, Janessa J.; Faculty of Medicine, University of British Columbia, Vancouver, British Columbia ; Lau, Harold Y.; Faculty of Medicine, University of Calgary, Calgary, Alberta ; Petrillo, Stephanie K.; Siever, Jodi E.; Thomson, Thomas A.; Faculty of Medicine, University of British Columbia, Vancouver, British Columbia ; Magliocco, Anthony M.; Faculty of Medicine, University of Calgary, Calgary, Alberta ; Hao, Desirée; Faculty of Medicine, University of Calgary, Calgary, Alberta

    2013-04-01

    Purpose: We sought to evaluate the prognostic/predictive value of ERCC1 and XPF in patients with nonmetastatic nasopharyngeal carcinoma (NPC) treated with curative intent. Methods and Materials: ERCC1 and XPF protein expression was evaluated by immunofluorescence combined with automated quantitative analysis (AQUA) using the FL297 and 3F2 antibodies, respectively. ERCC1 and XPF protein expression levels were correlated with clinical outcomes. Results: Patient characteristics were as follows: mean age 52 years (range, 18-85 years), 67% male, 72% Karnofsky performance status (KPS) ?90%, World Health Organization (WHO) type 1/2/3 = 12%/28%/60%, stage III/IV 65%. With a median follow-up time of 50 months (range, 2.9 to 120 months), the 5-year overall survival (OS) was 70.8%. Median standardized nuclear AQUA scores were used as cutpoints for ERCC1 (n=138) and XPF (n=130) protein expression. Agreement between dichotomized ERCC1 and XPF scores was high at 79.4% (kappa = 0.587, P<.001). Neither biomarker predicted locoregional recurrence, DFS, or OS after adjustment for age and KPS, irrespective of stratification by stage, WHO type, or treatment. Conclusions: Neither ERCC1 nor XPF, analyzed by quantitative immunohistochemistry using the FL297 and 3F2 antibodies, was prognostic or predictive in this cohort of NPC patients.

  13. Quantitative characterization of collagen in the fibrotic capsule surrounding implanted polymeric microparticles through second harmonic generation imaging

    SciTech Connect (OSTI)

    Akilbekova, Dana; Bratlie, Kaitlin M.; Abraham, Thomas

    2015-06-30

    The collagenous capsule formed around an implant will ultimately determine the nature of its in vivo fate. To provide a better understanding of how surface modifications can alter the collagen orientation and composition in the fibrotic capsule, we used second harmonic generation (SHG) microscopy to evaluate collagen organization and structure generated in mice subcutaneously injected with chemically functionalized polystyrene particles. SHG is sensitive to the orientation of a molecule, making it a powerful tool for measuring the alignment of collagen fibers. Additionally, SHG arises from the second order susceptibility of the interrogated molecule in response to the electric field. Variation in these tensor components distinguishes different molecular sources of SHG, providing collagen type specificity. Here, we demonstrated the ability of SHG to differentiate collagen type I and type III quantitatively and used this method to examine fibrous capsules of implanted polystyrene particles. Data presented in this work shows a wide range of collagen fiber orientations and collagen compositions in response to surface functionalized polystyrene particles. Dimethylamino functionalized particles were able to form a thin collagenous matrix resembling healthy skin. These findings have the potential to improve the fundamental understanding of how material properties influence collagen organization and composition quantitatively.

  14. Quantitative characterization of collagen in the fibrotic capsule surrounding implanted polymeric microparticles through second harmonic generation imaging

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Akilbekova, Dana; Bratlie, Kaitlin M.; Abraham, Thomas

    2015-06-30

    The collagenous capsule formed around an implant will ultimately determine the nature of its in vivo fate. To provide a better understanding of how surface modifications can alter the collagen orientation and composition in the fibrotic capsule, we used second harmonic generation (SHG) microscopy to evaluate collagen organization and structure generated in mice subcutaneously injected with chemically functionalized polystyrene particles. SHG is sensitive to the orientation of a molecule, making it a powerful tool for measuring the alignment of collagen fibers. Additionally, SHG arises from the second order susceptibility of the interrogated molecule in response to the electric field. Variationmore »in these tensor components distinguishes different molecular sources of SHG, providing collagen type specificity. Here, we demonstrated the ability of SHG to differentiate collagen type I and type III quantitatively and used this method to examine fibrous capsules of implanted polystyrene particles. Data presented in this work shows a wide range of collagen fiber orientations and collagen compositions in response to surface functionalized polystyrene particles. Dimethylamino functionalized particles were able to form a thin collagenous matrix resembling healthy skin. These findings have the potential to improve the fundamental understanding of how material properties influence collagen organization and composition quantitatively.« less

  15. Quantitative Chromatographic Determination of Dissolved Elemental Sulfur in the Non-aqueous Electrolyte for Lithium-Sulfur Batteries

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zheng, Dong; Yang, Xiao-Qing; Zhang, Xuran; Li, Chao; McKinnon, Meaghan E.; Sadok, Rachel G.; Qu, Deyu; Yu, Xiqian; Lee, Hung-Sui; Qu, Deyang

    2014-12-02

    A fast and reliable analytical method is reported for the quantitative determination of dissolved elemental sulfur in non-aqueous electrolytes for Li-S batteries. By using high performance liquid chromatography with a UV detector, the solubility of S in 12 different pure solvents and in 22 different electrolytes was determined. It was found that the solubility of elemental sulfur is dependent on the Lewis basicity, the polarity of solvents and the salt concentration in the electrolytes. In addition, the S content in the electrolyte recovered from a discharged Li-S battery was successfully determined by the proposed HPLC/UV method. Thus, the feasibility ofmore »the method to the online analysis for a Li-S battery is demonstrated. Interestingly, the S was found super-saturated in the electrolyte recovered from a discharged Li-S cell.« less

  16. Quantitative Chromatographic Determination of Dissolved Elemental Sulfur in the Non-aqueous Electrolyte for Lithium-Sulfur Batteries

    SciTech Connect (OSTI)

    Zheng, Dong [Univ. of Massachusetts, Boston, MA (United States). Dept. of Chemistry; Yang, Xiao-Qing [Brookhaven National Laboratory (BNL), Upton, NY (United States). Chemistry Dept.; Zhang, Xuran [Wuhan Univ. of Technology, Hubei (China); Dept. of Chemistry; Li, Chao [Univ. of Massachusetts, Boston, MA (United States). Dept. of Chemistry; McKinnon, Meaghan E. [Univ. of Massachusetts, Boston, MA (United States). Dept. of Chemistry; Sadok, Rachel G. [Univ. of Massachusetts, Boston, MA (United States). Dept. of Chemistry; Qu, Deyu [Wuhan Univ. of Technology, Hubei (China); Dept. of Chemistry; Yu, Xiqian [Brookhaven National Laboratory (BNL), Upton, NY (United States). Chemistry Dept.; Lee, Hung-Sui [Brookhaven National Laboratory (BNL), Upton, NY (United States). Chemistry Dept.; Qu, Deyang [Univ. of Massachusetts, Boston, MA (United States). Dept. of Chemistry

    2014-11-01

    A fast and reliable analytical method is reported for the quantitative determination of dissolved elemental sulfur in non-aqueous electrolytes for Li-S batteries. By using high performance liquid chromatography with a UV detector, the solubility of S in 12 different pure solvents and in 22 different electrolytes was determined. It was found that the solubility of elemental sulfur is dependent on the Lewis basicity, the polarity of solvents and the salt concentration in the electrolytes. In addition, the S content in the electrolyte recovered from a discharged Li-S battery was successfully determined by the proposed HPLC/UV method. Thus, the feasibility of the method to the online analysis for a Li-S battery is demonstrated. Interestingly, the S was found super-saturated in the electrolyte recovered from a discharged Li-S cell.

  17. Quantitative Chromatographic Determination of Dissolved Elemental Sulfur in the Non-aqueous Electrolyte for Lithium-Sulfur Batteries

    SciTech Connect (OSTI)

    Zheng, Dong; Yang, Xiao-Qing; Zhang, Xuran; Li, Chao; McKinnon, Meaghan E.; Sadok, Rachel G.; Qu, Deyu; Yu, Xiqian; Lee, Hung-Sui; Qu, Deyang

    2014-12-02

    A fast and reliable analytical method is reported for the quantitative determination of dissolved elemental sulfur in non-aqueous electrolytes for Li-S batteries. By using high performance liquid chromatography with a UV detector, the solubility of S in 12 different pure solvents and in 22 different electrolytes was determined. It was found that the solubility of elemental sulfur is dependent on the Lewis basicity, the polarity of solvents and the salt concentration in the electrolytes. In addition, the S content in the electrolyte recovered from a discharged Li-S battery was successfully determined by the proposed HPLC/UV method. Thus, the feasibility of the method to the online analysis for a Li-S battery is demonstrated. Interestingly, the S was found super-saturated in the electrolyte recovered from a discharged Li-S cell.

  18. UPDATE February 2012 - The Food Crises: Predictive validation of a quantitative model of food prices including speculators and ethanol conversion

    E-Print Network [OSTI]

    Lagi, Marco; Bertrand, Karla Z; Bar-Yam, Yaneer

    2012-01-01

    Increases in global food prices have led to widespread hunger and social unrest---and an imperative to understand their causes. In a previous paper published in September 2011, we constructed for the first time a dynamic model that quantitatively agreed with food prices. Specifically, the model fit the FAO Food Price Index time series from January 2004 to March 2011, inclusive. The results showed that the dominant causes of price increases during this period were investor speculation and ethanol conversion. The model included investor trend following as well as shifting between commodities, equities and bonds to take advantage of increased expected returns. Here, we extend the food prices model to January 2012, without modifying the model but simply continuing its dynamics. The agreement is still precise, validating both the descriptive and predictive abilities of the analysis. Policy actions are needed to avoid a third speculative bubble that would cause prices to rise above recent peaks by the end of 2012.

  19. 19/11/2007 10:18Bacteria display human traits like pursuing their own short term inte...s researchers found.But bugs also co-operate for the good of their... Page 1 of 2http://www.thisisnottingham.co.uk/displayNode.jsp?nodeId=133965...entPK=18980737&modul

    E-Print Network [OSTI]

    West, Stuart

    19/11/2007 10:18Bacteria display human traits like pursuing their own short term inte emails Sign up for newsflashes Order a copy of the newspaper back to list BACTERIA LINK TO HUMAN FRAILTIES Be the first reader to comment on this story 09:00 - 16 November 2007 Bacteria display human

  20. VHF EPR quantitation and speciation of organic sulfur in coal. Technical report, 1 March--31 May 1994

    SciTech Connect (OSTI)

    Clarkson, R.B.; Belford, R.L.

    1994-09-01

    The existence of free electrons in coals` natural state offers a great attraction for Electron Paramagnetic Resonance (EPR) analysis to aid in the study of the structure and composition of coal. This direct and non-destructive approach to coal analysis has been hindered by the problem of resolution using the conventional 9.5 GHz EPR spectrometers. In the past few years, the authors have developed techniques including W-band Very High Frequency EPR spectroscopy as a means of determining the quantity and structure of organic sulfur in native and desulfurized coals. The state-of-the-art 95 GHz (W-band) EPR spectrometer which they have constructed shows a well resolved spectrum including the interaction between unpaired electrons and the heteroatom like sulfur. The spectra also provide quantitative as well as qualitative information regarding different sulfur species. In this quarter, the authors have been concentrating their efforts on developing a new standard protocol in handling and preparing the coal samples for EPR measurements to provide a quantitative comparison between the EPR spectra of coal in the natural state and desulfurized. Sixteen new coal samples, both native and desulfurized, have been provided to us as well as to the University of Kentucky for analysis by XANES. These samples have been run in both laboratories. The results from these samples, which were kept in an oxygen-free environment, are compared to those of 10 previous samples, which were air-oxidized. Significant differences in the EPR spectra of air-oxidized and oxygen free samples are noted; results from Kentucky are not yet available. Desulfurized samples show a significant decrease in organic sulfur as measured by the VHF-EPR method.

  1. Approaches to advancing quantitative human health risk assessment of environmental chemicals in the post-genomic era

    SciTech Connect (OSTI)

    Chiu, Weihsueh A.; Euling, Susan Y.; Scott, Cheryl Siegel; Subramaniam, Ravi P.

    2013-09-15

    The contribution of genomics and associated technologies to human health risk assessment for environmental chemicals has focused largely on elucidating mechanisms of toxicity, as discussed in other articles in this issue. However, there is interest in moving beyond hazard characterization to making more direct impacts on quantitative risk assessment (QRA) — i.e., the determination of toxicity values for setting exposure standards and cleanup values. We propose that the evolution of QRA of environmental chemicals in the post-genomic era will involve three, somewhat overlapping phases in which different types of approaches begin to mature. The initial focus (in Phase I) has been and continues to be on “augmentation” of weight of evidence — using genomic and related technologies qualitatively to increase the confidence in and scientific basis of the results of QRA. Efforts aimed towards “integration” of these data with traditional animal-based approaches, in particular quantitative predictors, or surrogates, for the in vivo toxicity data to which they have been anchored are just beginning to be explored now (in Phase II). In parallel, there is a recognized need for “expansion” of the use of established biomarkers of susceptibility or risk of human diseases and disorders for QRA, particularly for addressing the issues of cumulative assessment and population risk. Ultimately (in Phase III), substantial further advances could be realized by the development of novel molecular and pathway-based biomarkers and statistical and in silico models that build on anticipated progress in understanding the pathways of human diseases and disorders. Such efforts would facilitate a gradual “reorientation” of QRA towards approaches that more directly link environmental exposures to human outcomes.

  2. MethodsMethods ! Analyzed 10-15 nuclear microsatellite loci

    E-Print Network [OSTI]

    Gottgens, Hans

    Mississippi River Proportion private alleles HeterozygosityNSite #12;Example: Allelic variation amongExample: Allelic variation among walleye population siteswalleye population sites #12;Example: Allelic variation amongExample: Allelic variation among walleye population siteswalleye population sites atat SviSvi L7

  3. Volumes of certain loci of polynomials and their applicatoins 

    E-Print Network [OSTI]

    Sethuraman, Swaminathan

    2010-01-16

    polynomials in n variables with k = 4 fixed those that are SOS occupy a vanishingly small fraction of those that are nonnegative on Rn, as n -> 1. With an eye toward the case of small n, we refine Blekherman'[s bounds by incorporating the underlying Newton...

  4. On Combinatorics of Degeneracy Loci Alexander T F Yong

    E-Print Network [OSTI]

    Yong, Alexander

    to thank Alexander Barvinok, Phil Hanlon and the Rackham Graduate school for their #12;nancial support Knutson, Ezra Miller and Mark Shimozono for sharing details of their work [27]. I am particularly grateful in the proof of Theorem 1. I also would like to thank Mark Haiman for explaining [5] to me. As to Chapter 3, I

  5. Towards the identification of the loci of adaptive 2 evolution

    E-Print Network [OSTI]

    Pardo-Diaz, Carolina; Salazar, Camilo; Jiggins, Chris D.

    2015-02-12

    that has helped understanding the genetics underlying flower coloration and other evolution- ary processes, including the role of gene duplication in the evo- lution of novel phenotypes (Des Marais & Rausher 2008), causes of evolutionary rate variation...

  6. MICROSATELLITE LETTERS Isolation of sixteen microsatellite loci for Rhoadsia altipinna

    E-Print Network [OSTI]

    Aguirre, Windsor E.

    Facultad de Ingenieria Mari´tima, Ciencias Biolo´gicas Ocea´nicas y Recursos Naturales, Escuela Superior

  7. TECHNICAL NOTE Polymorphic microsatellite loci from the endangered Giant Otter

    E-Print Network [OSTI]

    Solé-Cava, Antonio M.

    double-stranded linker C. Ribas (&) Laborato´rio de Biodiversidade Molecular, Departamento de Gene´tica. Vasconcellos Á A. M. Sole´-Cava Á H. A. Cunha Laborato´rio de Biodiversidade Molecular, Departamento de Gene´tica

  8. Rangewide Genetic Structure in Paddlefish Inferred from DNA Microsatellite Loci

    E-Print Network [OSTI]

    Heist, Edward J.

    populations that have more recently become isolated by habitat alteration (e.g., dams). Although we do, China, where it is threatened with extinction due to habitat alteration (e.g., dams and pollution to list paddlefish under the U.S. Endangered Species Act in 1989. However, concerns about declining stocks

  9. TECHNICAL NOTE Highly polymorphic microsatellite loci for the Parsley frog

    E-Print Network [OSTI]

    Borges, Rita

    of predators (Coll- inson et al. 1995). Mediterranean temporary ponds are suffering widespread degradation of the Algarve (Portugal), was isolated by using a standard phenol- chloroform extraction protocol (Sambrook et

  10. Quantitative cone-beam CT imaging in radiation therapy using planning CT as a prior: First patient studies

    SciTech Connect (OSTI)

    Niu Tianye; Al-Basheer, Ahmad; Zhu Lei [Nuclear and Radiological Engineering and Medical Physics Programs, George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 (United States); Georgia Radiation Therapy Center, Department of Radiology, Georgia Health Sciences University, Augusta, Georgia 30912 (United States); Nuclear and Radiological Engineering and Medical Physics Programs, George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 (United States)

    2012-04-15

    Purpose: Quantitative cone-beam CT (CBCT) imaging is on increasing demand for high-performance image guided radiation therapy (IGRT). However, the current CBCT has poor image qualities mainly due to scatter contamination. Its current clinical application is therefore limited to patient setup based on only bony structures. To improve CBCT imaging for quantitative use, we recently proposed a correction method using planning CT (pCT) as the prior knowledge. Promising phantom results have been obtained on a tabletop CBCT system, using a correction scheme with rigid registration and without iterations. More challenges arise in clinical implementations of our method, especially because patients have large organ deformation in different scans. In this paper, we propose an improved framework to extend our method from bench to bedside by including several new components. Methods: The basic principle of our correction algorithm is to estimate the primary signals of CBCT projections via forward projection on the pCT image, and then to obtain the low-frequency errors in CBCT raw projections by subtracting the estimated primary signals and low-pass filtering. We improve the algorithm by using deformable registration to minimize the geometry difference between the pCT and the CBCT images. Since the registration performance relies on the accuracy of the CBCT image, we design an optional iterative scheme to update the CBCT image used in the registration. Large correction errors result from the mismatched objects in the pCT and the CBCT scans. Another optional step of gas pocket and couch matching is added into the framework to reduce these effects. Results: The proposed method is evaluated on four prostate patients, of which two cases are presented in detail to investigate the method performance for a large variety of patient geometry in clinical practice. The first patient has small anatomical changes from the planning to the treatment room. Our algorithm works well even without the optional iterations and the gas pocket and couch matching. The image correction on the second patient is more challenging due to the effects of gas pockets and attenuating couch. The improved framework with all new components is used to fully evaluate the correction performance. The enhanced image quality has been evaluated using mean CT number and spatial nonuniformity (SNU) error as well as contrast improvement factor. If the pCT image is considered as the ground truth, on the four patients, the overall mean CT number error is reduced from over 300 HU to below 16 HU in the selected regions of interest (ROIs), and the SNU error is suppressed from over 18% to below 2%. The average soft-tissue contrast is improved by an average factor of 2.6. Conclusions: We further improve our pCT-based CBCT correction algorithm for clinical use. Superior correction performance has been demonstrated on four patient studies. By providing quantitative CBCT images, our approach significantly increases the accuracy of advanced CBCT-based clinical applications for IGRT.

  11. A quantitative method to analyze the quality of EIA information in wind energy development and avian/bat assessments

    SciTech Connect (OSTI)

    Chang, Tony, E-mail: tc282@nau.edu [Environmental Science and Policy Program, School of Earth Science and Environmental Sustainability, Northern Arizona University, 602 S Humphreys P.O. Box 5694, Flagstaff, AZ, 86011 (United States); Nielsen, Erik, E-mail: erik.nielsen@nau.edu [Environmental Science and Policy Program, School of Earth Science and Environmental Sustainability, Northern Arizona University, 602 S Humphreys P.O. Box 5694, Flagstaff, AZ, 86011 (United States); Auberle, William, E-mail: william.auberle@nau.edu [Civil and Environmental Engineering Program, Department of Civil and Environmental Engineering, Northern Arizona University, 2112 S Huffer Ln P.O. Box 15600, Flagstaff, AZ, 860011 (United States); Solop, Frederic I., E-mail: fred.solop@nau.edu [Political Science Program, Department of Politics and International Affairs, Northern Arizona University, P.O. Box 15036, Flagstaff, AZ 86001 (United States)

    2013-01-15

    The environmental impact assessment (EIA) has been a tool for decision makers since the enactment of the National Environmental Policy Act (NEPA). Since that time, few analyses have been performed to verify the quality of information and content within EIAs. High quality information within assessments is vital in order for decision makers, stake holders, and the public to understand the potential impact of proposed actions on the ecosystem and wildlife species. Low quality information has been a major cause for litigation and economic loss. Since 1999, wind energy development has seen an exponential growth with unknown levels of impact on wildlife species, in particular bird and bat species. The purpose of this article is to: (1) develop, validate, and apply a quantitative index to review avian/bat assessment quality for wind energy EIAs; and (2) assess the trends and status of avian/bat assessment quality in a sample of wind energy EIAs. This research presents the development and testing of the Avian and Bat Assessment Quality Index (ABAQI), a new approach to quantify information quality of ecological assessments within wind energy development EIAs in relation to avian and bat species based on review areas and factors derived from 23 state wind/wildlife siting guidance documents. The ABAQI was tested through a review of 49 publicly available EIA documents and validated by identifying high variation in avian and bat assessments quality for wind energy developments. Of all the reviewed EIAs, 66% failed to provide high levels of preconstruction avian and bat survey information, compared to recommended factors from state guidelines. This suggests the need for greater consistency from recommended guidelines by state, and mandatory compliance by EIA preparers to avoid possible habitat and species loss, wind energy development shut down, and future lawsuits. - Highlights: Black-Right-Pointing-Pointer We developed, validated, and applied a quantitative index to review avian/bat assessment quality for wind energy EIAs. Black-Right-Pointing-Pointer We assessed the trends and status of avian/bat assessment quality in a sample of wind energy EIAs. Black-Right-Pointing-Pointer Applied index to 49 EIA documents and identified high variation in assessment quality for wind energy developments. Black-Right-Pointing-Pointer For the reviewed EIAs, 66% provided inadequate preconstruction avian and bat survey information.

  12. Koon Leai Larry Tan, Paul S. Lambert, Vernon Gayle and Kenneth J. Turner Enabling Quantitative Data Analysis on Cyberinfrastructures and Grids.

    E-Print Network [OSTI]

    Turner, Ken

    Analysis on Cyberinfrastructures and Grids. In Gary Olson and Rob Procter, editors, Proc. 3rd International Quantitative Data Analysis on Cyberinfrastructures and Grids Koon Leai Larry Tan1 , Paul Lambert2 , Vernon data storage, data management and data analysis do exist, for example the NESSTAR, IPUMS and LIS

  13. Quantitative and Qualitative Determination of Polysulfide Species in the Electrolyte of a Lithium-Sulfur Battery using HPLC ESI/MS with One-Step Derivatization

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zheng, Dong; Qu, Deyu; Yang, Xiao-Qing; Yu, Xiqian; Lee, Hung-Sui; Qu, Deyang

    2015-01-29

    The polysulfide species dissolved in aprotic solvents can be separated and analyzed by reverse phase (RP) high performance liquid chromatography (HPLC) in tandem with electrospray-mass spectroscopy. The relative distribution of polysulfide species in the electrolyte recovered from Li-S batteries is quantitatively and reliably determined for the first time.

  14. GEOPHYSICAL RESEARCH LETTERS, VOL. 28, NO. 9, PAGES 1887-1890, MAY 1, 2001 Quantitative Prediction of Radiation Belt Electrons at

    E-Print Network [OSTI]

    California at Berkeley, University of

    . N. Baker,1 G. D. Reeves3 and D. Larson2 Abstract. Solar wind measurements are used to predict the Me measured at geostationary orbit with our prediction based solely on measurements of the solar wind. BothGEOPHYSICAL RESEARCH LETTERS, VOL. 28, NO. 9, PAGES 1887-1890, MAY 1, 2001 Quantitative Prediction

  15. GEOPHYSICAL RESEARCH LETTERS, VOL. 0, NO. 0, PAGES 0-0, M 0, 2001 Quantitative Prediction of Radiation Belt Electrons at

    E-Print Network [OSTI]

    Li, Xinlin

    . N. Baker,1 G. D. Reeves3 and D. Larson2 Abstract. Solar wind measurements are used to predict the Me measured at geostationary orbit with our prediction based solely on measurements of the solar wind. BothGEOPHYSICAL RESEARCH LETTERS, VOL. 0, NO. 0, PAGES 0-0, M 0, 2001 Quantitative Prediction

  16. Approved Module Information for BFM114, 2014/5 Module Title/Name: Quantitative Methods for Finance Module Code: BFM114

    E-Print Network [OSTI]

    Neirotti, Juan Pablo

    Approved Module Information for BFM114, 2014/5 Module Title/Name: Quantitative Methods for Finance Module Code: BFM114 School: Aston Business School Module Type: Standard Module New Module? No Module Credits: 15 Module Management Information Module Leader Name D Gilder Email Address gilderd

  17. Quantitative strain mapping of InAs/InP quantum dots with 1 nm spatial resolution using dark field electron holography

    E-Print Network [OSTI]

    Dunin-Borkowski, Rafal E.

    used to measure the strain in InAs quantum dots grown in InP with a spatial resolution of 1 nmQuantitative strain mapping of InAs/InP quantum dots with 1 nm spatial resolution using dark field of semiconductor quantum dots are greatly influenced by their strain state. Dark field electron holography has been

  18. Quantitative and Qualitative Determination of Polysulfide Species in the Electrolyte of a Lithium-Sulfur Battery using HPLC ESI/MS with One-Step Derivatization

    SciTech Connect (OSTI)

    Zheng, Dong [Department of Chemistry; University of Massachusetts Boston; Boston MA 02125 USA; Qu, Deyu [Wuhan Univ. of Technology, Wuhan (China). Dept. of Chemistry; Yang, Xiao-Qing [Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Chemistry; Yu, Xiqian [Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Chemistry; Lee, Hung-Sui [Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Chemistry; Qu, Deyang [Univ. of Massachusetts, Boston, MA (United States). Dept. of Chemistry

    2015-01-01

    The polysulfide species dissolved in aprotic solvents can be separated and analyzed by reverse phase (RP) high performance liquid chromatography (HPLC) in tandem with electrospray-mass spectroscopy. The relative distribution of polysulfide species in the electrolyte recovered from Li-S batteries is quantitatively and reliably determined for the first time.

  19. Climate change has been one of the major issues of global economic and social concerns in the past decade. To quantitatively predict global climate change, the Intergovernmental

    E-Print Network [OSTI]

    Climate change has been one of the major issues of global economic and social concerns in the past decade. To quantitatively predict global climate change, the Intergovernmental Panel on Climate Change-ocean models to project anthropogenically induced climate changes in the 21st century. The computer simulations

  20. Noninvasive and quantitative evaluation of bone marrow infiltration and bone marrow microcirculation in patients with monoclonal plasma cell disease by means of diffusion

    E-Print Network [OSTI]

    Gertz, Michael

    .0001) and D (p study clearly shows that bone marrow infiltrationRajiv Shah Dr. med. Noninvasive and quantitative evaluation of bone marrow infiltration and bone diseases. The response to treatment is evaluated mostly on the basis of hematologic parameters. This study

  1. THE EVOLUTION OF SOLAR FLUX FROM 0.1 nm TO 160 {mu}m: QUANTITATIVE ESTIMATES FOR PLANETARY STUDIES

    SciTech Connect (OSTI)

    Claire, Mark W.; Sheets, John; Meadows, Victoria S.; Cohen, Martin; Ribas, Ignasi; Catling, David C.

    2012-09-20

    Understanding changes in the solar flux over geologic time is vital for understanding the evolution of planetary atmospheres because it affects atmospheric escape and chemistry, as well as climate. We describe a numerical parameterization for wavelength-dependent changes to the non-attenuated solar flux appropriate for most times and places in the solar system. We combine data from the Sun and solar analogs to estimate enhanced UV and X-ray fluxes for the young Sun and use standard solar models to estimate changing visible and infrared fluxes. The parameterization, a series of multipliers relative to the modern top of the atmosphere flux at Earth, is valid from 0.1 nm through the infrared, and from 0.6 Gyr through 6.7 Gyr, and is extended from the solar zero-age main sequence to 8.0 Gyr subject to additional uncertainties. The parameterization is applied to a representative modern day flux, providing quantitative estimates of the wavelength dependence of solar flux for paleodates relevant to the evolution of atmospheres in the solar system (or around other G-type stars). We validate the code by Monte Carlo analysis of uncertainties in stellar age and flux, and with comparisons to the solar proxies {kappa}{sup 1} Cet and EK Dra. The model is applied to the computation of photolysis rates on the Archean Earth.

  2. Apparatus and method for qualitative and quantitative measurements of optical properties of turbid media using frequency-domain photon migration

    DOE Patents [OSTI]

    Tromberg, Bruce J. (Irvine, CA); Tsay, Tsong T. (Orange, CA); Berns, Michael W. (Trabuco Canyon, CA); Svaasand, Lara O. (Rudolf Raedersu, NO); Haskell, Richard C. (Claremont, CA)

    1995-01-01

    Optical measurements of turbid media, that is media characterized by multiple light scattering, is provided through an apparatus and method for exposing a sample to a modulated laser beam. The light beam is modulated at a fundamental frequency and at a plurality of integer harmonics thereof. Modulated light is returned from the sample and preferentially detected at cross frequencies at frequencies slightly higher than the fundamental frequency and at integer harmonics of the same. The received radiance at the beat or cross frequencies is compared against a reference signal to provide a measure of the phase lag of the radiance and modulation ratio relative to a reference beam. The phase and modulation amplitude are then provided as a frequency spectrum by an array processor to which a computer applies a complete curve fit in the case of highly scattering samples or a linear curve fit below a predetermined frequency in the case of highly absorptive samples. The curve fit in any case is determined by the absorption and scattering coefficients together with a concentration of the active substance in the sample. Therefore, the curve fitting to the frequency spectrum can be used both for qualitative and quantitative analysis of substances in the sample even though the sample is highly turbid.

  3. Apparatus and method for qualitative and quantitative measurements of optical properties of turbid media using frequency-domain photon migration

    DOE Patents [OSTI]

    Tromberg, B.J.; Tsay, T.T.; Berns, M.W.; Svaasand, L.O.; Haskell, R.C.

    1995-06-13

    Optical measurements of turbid media, that is media characterized by multiple light scattering, is provided through an apparatus and method for exposing a sample to a modulated laser beam. The light beam is modulated at a fundamental frequency and at a plurality of integer harmonics thereof. Modulated light is returned from the sample and preferentially detected at cross frequencies at frequencies slightly higher than the fundamental frequency and at integer harmonics of the same. The received radiance at the beat or cross frequencies is compared against a reference signal to provide a measure of the phase lag of the radiance and modulation ratio relative to a reference beam. The phase and modulation amplitude are then provided as a frequency spectrum by an array processor to which a computer applies a complete curve fit in the case of highly scattering samples or a linear curve fit below a predetermined frequency in the case of highly absorptive samples. The curve fit in any case is determined by the absorption and scattering coefficients together with a concentration of the active substance in the sample. Therefore, the curve fitting to the frequency spectrum can be used both for qualitative and quantitative analysis of substances in the sample even though the sample is highly turbid. 14 figs.

  4. Cancer-associated p53 tetramerization domain mutants: quantitative analysis reveals a low threshold for tumor suppressor inactivation

    SciTech Connect (OSTI)

    Kamada, R.; Anderson, C.; Nomura, T.; Sakaguchi, K.

    2011-01-07

    The tumor suppressor p53, a 393-amino acid transcription factor, induces cell cycle arrest and apoptosis in response to genotoxic stress. Its inactivation via the mutation of its gene is a key step in tumor progression, and tetramer formation is critical for p53 post-translational modification and its ability to activate or repress the transcription of target genes vital in inhibiting tumor growth. About 50% of human tumors have TP53 gene mutations; most are missense ones that presumably lower the tumor suppressor activity of p53. In this study, we explored the effects of known tumor-derived missense mutations on the stability and oligomeric structure of p53; our comprehensive, quantitative analyses encompassed the tetramerization domain peptides representing 49 such substitutions in humans. Their effects on tetrameric structure were broad, and the stability of the mutant peptides varied widely ({Delta}T{sub m} = 4.8 {approx} -46.8 C). Because formation of a tetrameric structure is critical for protein-protein interactions, DNA binding, and the post-translational modification of p53, a small destabilization of the tetrameric structure could result in dysfunction of tumor suppressor activity. We suggest that the threshold for loss of tumor suppressor activity in terms of the disruption of the tetrameric structure of p53 could be extremely low. However, other properties of the tetramerization domain, such as electrostatic surface potential and its ability to bind partner proteins, also may be important.

  5. TRACKING CORONAL FEATURES FROM THE LOW CORONA TO EARTH: A QUANTITATIVE ANALYSIS OF THE 2008 DECEMBER 12 CORONAL MASS EJECTION

    SciTech Connect (OSTI)

    DeForest, C. E.; Howard, T. A.; McComas, D. J.

    2013-05-20

    We have tracked a slow magnetic cloud associated coronal mass ejection (CME) continuously from its origin as a flux rope structure in the low solar corona over a four-day passage to impact with spacecraft located near Earth. Combining measurements from the STEREO, ACE, and Wind space missions, we are able to follow major elements with enough specificity to relate pre-CME coronal structure in the low corona to the corresponding elements seen in the near-Earth in situ data. Combining extreme ultraviolet imaging, quantitative Thomson scattering data throughout the flight of the CME, and ''ground-truth'' in situ measurements, we: (1) identify the plasma observed by ACE and Wind with specific features in the solar corona (a segment of a long flux rope); (2) determine the onset mechanism of the CME (destabilization of a filament channel following flare reconnection, coupled with the mass draining instability) and demonstrate that it is consistent with the in situ measurements; (3) identify the origin of different layers of the sheath material around the central magnetic cloud (closed field lifted from the base of the corona, closed field entrained during passage through the corona, and solar wind entrained by the front of the CME); (4) measure mass accretion of the system via snowplow effects in the solar wind as the CME crossed the solar system; and (5) quantify the kinetic energy budget of the system in interplanetary space, and determine that it is consistent with no long-term driving force on the CME.

  6. Microfluidics for quantitative biology

    E-Print Network [OSTI]

    Adler, Micha

    2012-01-01

    X. Y. Jiang, et al. , Analytical Chemistry 76, 2273 D. M.J. Wieder, et al. , Analytical Chemistry 76, P. J. Hung, P.E. Sahlin, et al. , Analytical Chemistry 77, 3897 (2005). U.

  7. Microfluidics for quantitative biology

    E-Print Network [OSTI]

    Adler, Micha

    2012-01-01

    by controlling volumetric fluxes with syringe pumps. 14, 24pumps, which are commonly used to control volumetric fluxes,

  8. Microfluidics for quantitative biology

    E-Print Network [OSTI]

    Adler, Micha

    2012-01-01

    of plastic tubing (Tygon or PTFE) which is connected to thestopper. A hard-plastic (PTFE) tubing is threaded throughdevice through a line of PTFE tubing using a specially made

  9. CHEMISTRY 330 QUANTITATIVE ANALYSIS

    E-Print Network [OSTI]

    Nickrent, Daniel L.

    and evaluation of error, basic chemical equilibria, acid-base equilibria, solubility product constant equilibria of spectrophotometric analysis, atomic absorption spectroscopy, methods of separation, gas and liquid chromatography to develop and apply topics discussed in lecture; short quizzes may be used to assess individual learning

  10. Statistically Quantitative Volume Visualization

    E-Print Network [OSTI]

    Kniss, Joe Michael

    in the visualization pipeline involves some level of uncertainty, this valuable quantity should accessible as part for volume visualization ­ Risk transfer functions ­ Visual uncertainty ­ Interactive decision manipulation

  11. Quantitative luminescence imaging system

    DOE Patents [OSTI]

    Erwin, David N. (San Antonio, TX); Kiel, Johnathan L. (San Antonio, TX); Batishko, Charles R. (West Richland, WA); Stahl, Kurt A. (Richland, WA)

    1990-01-01

    The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopie imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber.

  12. Quantitative luminescence imaging system

    DOE Patents [OSTI]

    Erwin, D.N.; Kiel, J.L.; Batishko, C.R.; Stahl, K.A.

    1990-08-14

    The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopic imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber. 22 figs.

  13. Quantitative comparison between PGNAA measurements and MCNP calculations in view of the characterization of radioactive wastes in Germany and France

    SciTech Connect (OSTI)

    Mauerhofer, E.; Havenith, A.; Kettler, J.; Carasco, C.; Payan, E.; Ma, J. L.; Perot, B.

    2013-04-19

    The Forschungszentrum Juelich GmbH (FZJ), together with the Aachen University Rheinisch-Westfaelische Technische Hochschule (RWTH) and the French Alternative Energies and Atomic Energy Commission (CEA Cadarache) are involved in a cooperation aiming at characterizing toxic and reactive elements in radioactive waste packages by means of Prompt Gamma Neutron Activation Analysis (PGNAA). The French and German waste management agencies have indeed defined acceptability limits concerning these elements in view of their projected geological repositories. A first measurement campaign was performed in the new Prompt Gamma Neutron Activation Analysis (PGNAA) facility called MEDINA, at FZJ, to assess the capture gamma-ray signatures of some elements of interest in large samples up to waste drums with a volume of 200 liter. MEDINA is the acronym for Multi Element Detection based on Instrumental Neutron Activation. This paper presents MCNP calculations of the MEDINA facility and quantitative comparison between measurement and simulation. Passive gamma-ray spectra acquired with a high purity germanium detector and calibration sources are used to qualify the numerical model of the crystal. Active PGNAA spectra of a sodium chloride sample measured with MEDINA then allow for qualifying the global numerical model of the measurement cell. Chlorine indeed constitutes a usual reference with reliable capture gamma-ray production data. The goal is to characterize the entire simulation protocol (geometrical model, nuclear data, and postprocessing tools) which will be used for current measurement interpretation, extrapolation of the performances to other types of waste packages or other applications, as well as for the study of future PGNAA facilities.

  14. Triaxially deformed relativistic point-coupling model for $?$ hypernuclei: a quantitative analysis of hyperon impurity effect on nuclear collective properties

    E-Print Network [OSTI]

    W. X. Xue; J. M. Yao; K. Hagino; Z. P. Li; H. Mei; Y. Tanimura

    2015-02-18

    The impurity effect of hyperon on atomic nuclei has received a renewed interest in nuclear physics since the first experimental observation of appreciable reduction of $E2$ transition strength in low-lying states of hypernucleus $^{7}_\\Lambda$Li. Many more data on low-lying states of $\\Lambda$ hypernuclei will be measured soon for $sd$-shell nuclei, providing good opportunities to study the $\\Lambda$ impurity effect on nuclear low-energy excitations. We carry out a quantitative analysis of $\\Lambda$ hyperon impurity effect on the low-lying states of $sd$-shell nuclei at the beyond-mean-field level based on a relativistic point-coupling energy density functional (EDF), considering that the $\\Lambda$ hyperon is injected into the lowest positive-parity ($\\Lambda_s$) and negative-parity ($\\Lambda_p$) states. We adopt a triaxially deformed relativistic mean-field (RMF) approach for hypernuclei and calculate the $\\Lambda$ binding energies of hypernuclei as well as the potential energy surfaces (PESs) in $(\\beta, \\gamma)$ deformation plane. We also calculate the PESs for the $\\Lambda$ hypernuclei with good quantum numbers using a microscopic particle rotor model (PRM) with the same relativistic EDF. The triaxially deformed RMF approach is further applied in order to determine the parameters of a five-dimensional collective Hamiltonian (5DCH) for the collective excitations of triaxially deformed core nuclei. Taking $^{25,27}_{\\Lambda}$Mg and $^{31}_{\\Lambda}$Si as examples, we analyse the impurity effects of $\\Lambda_s$ and $\\Lambda_p$ on the low-lying states of the core nuclei...

  15. Characterization and analysis methods for the examination of the heterogeneous solid oxide fuel cell electrode microstructure: Part 2. Quantitative measurement of the microstructure and contributions to transport losses

    SciTech Connect (OSTI)

    Grew, Kyle N.; Peracchio, Aldo A.; Chiu, W. K. S.

    2010-12-15

    Advanced characterization and analysis of multifunctional materials, such as the materials found in heterogeneous solid oxide fuel cell (SOFC) electrode architectures, can help to provide a qualitative and quantitative understanding of how these structures respond to different manufacturing and operating practices. Dense, opaque materials, which have large X-ray mass absorption coefficients and features on sub-micrometer length scales, can make characterization difficult. Advances in tomographic X-ray imaging can permit this level of detailed characterization, and complement stereographic scanning electron microscope measurements that have also been reported. In this second part of a two-part study, details regarding quantitative characterization methods that have been used to examine the SOFC anode microstructure are reported. The detailed formulation and validation of a phase size distributions for the three constitutive phases, as well as resistive loss microstructure-induced resistive loss distributions in the nickel (Ni) and yttria-stabilized zirconia (YSZ) phases are provided in this section.

  16. Population Distributions of APOE, APOH, and APOA4 Polymorphisms and Their Relationships with Quantitative Plasma Lipid Levels among the Evenki Herders of Siberia

    E-Print Network [OSTI]

    Kamboh, M. Ilyas; Crawford, Michael H.; Aston, Christopher E.; Leonard, William R.

    1999-04-01

    Population Distributions of APOE, APOH, and APOA4 Polymorphisms and Their Relationships with Quantitative Plasma Lipid Levels among the Evenki Herders of Siberia M. ILYAS KAMBOH, 1 MICHAEL H. CRAWFORD, 2 CHRISTOPHER E. ASTON, 1 AND WILLIAM R... of the APOH*3 allele, the complete absence of the APOA*2 allele at codon 360, and significantly different frequencies at three other APOA4 polymorphic sites. Our analyses of the relationships of common apoli-poprotein polymorphism and plasma lipid levels also...

  17. SU-E-QI-17: Dependence of 3D/4D PET Quantitative Image Features On Noise

    SciTech Connect (OSTI)

    Oliver, J; Budzevich, M; Zhang, G; Latifi, K; Dilling, T; Balagurunathan, Y; Gu, Y; Grove, O; Feygelman, V; Gillies, R; Moros, E; Lee, H.

    2014-06-15

    Purpose: Quantitative imaging is a fast evolving discipline where a large number of features are extracted from images; i.e., radiomics. Some features have been shown to have diagnostic, prognostic and predictive value. However, they are sensitive to acquisition and processing factors; e.g., noise. In this study noise was added to positron emission tomography (PET) images to determine how features were affected by noise. Methods: Three levels of Gaussian noise were added to 8 lung cancer patients PET images acquired in 3D mode (static) and using respiratory tracking (4D); for the latter images from one of 10 phases were used. A total of 62 features: 14 shape, 19 intensity (1stO), 18 GLCM textures (2ndO; from grey level co-occurrence matrices) and 11 RLM textures (2ndO; from run-length matrices) features were extracted from segmented tumors. Dimensions of GLCM were 256×256, calculated using 3D images with a step size of 1 voxel in 13 directions. Grey levels were binned into 256 levels for RLM and features were calculated in all 13 directions. Results: Feature variation generally increased with noise. Shape features were the most stable while RLM were the most unstable. Intensity and GLCM features performed well; the latter being more robust. The most stable 1stO features were compactness, maximum and minimum length, standard deviation, root-mean-squared, I30, V10-V90, and entropy. The most stable 2ndO features were entropy, sum-average, sum-entropy, difference-average, difference-variance, difference-entropy, information-correlation-2, short-run-emphasis, long-run-emphasis, and run-percentage. In general, features computed from images from one of the phases of 4D scans were more stable than from 3D scans. Conclusion: This study shows the need to characterize image features carefully before they are used in research and medical applications. It also shows that the performance of features, and thereby feature selection, may be assessed in part by noise analysis.

  18. Implications of CT noise and artifacts for quantitative {sup 99m}Tc SPECT/CT imaging

    SciTech Connect (OSTI)

    Hulme, K. W.; Kappadath, S. C.

    2014-04-15

    Purpose: This paper evaluates the effects of computed tomography (CT) image noise and artifacts on quantitative single-photon emission computed-tomography (SPECT) imaging, with the aim of establishing an appropriate range of CT acquisition parameters for low-dose protocols with respect to accurate SPECT attenuation correction (AC). Methods: SPECT images of two geometric and one anthropomorphic phantom were reconstructed iteratively using CT scans acquired at a range of dose levels (CTDI{sub vol} = 0.4 to 46 mGy). Resultant SPECT image quality was evaluated by comparing mean signal, background noise, and artifacts to SPECT images reconstructed using the highest dose CT for AC. Noise injection was performed on linear-attenuation (?) maps to determine the CT noise threshold for accurate AC. Results: High levels of CT noise (? ? 200–400 HU) resulted in low ?-maps noise (? ? 1%–3%). Noise levels greater than ?10% in 140 keV ?-maps were required to produce visibly perceptible increases of ?15% in {sup 99m}Tc SPECT images. These noise levels would be achieved at low CT dose levels (CTDI{sub vol} = 4 ?Gy) that are over 2 orders of magnitude lower than the minimum dose for diagnostic CT scanners. CT noise could also lower (bias) the expected ? values. The relative error in reconstructed SPECT signal trended linearly with the relative shift in ?. SPECT signal was, on average, underestimated in regions corresponding with beam-hardening artifacts in CT images. Any process that has the potential to change the CT number of a region by ?100 HU (e.g., misregistration between CT images and SPECT images due to motion, the presence of contrast in CT images) could introduce errors in ?{sub 140} {sub keV} on the order of 10%, that in turn, could introduce errors on the order of ?10% into the reconstructed {sup 99m}Tc SPECT image. Conclusions: The impact of CT noise on SPECT noise was demonstrated to be negligible for clinically achievable CT parameters. Because CT dose levels that affect SPECT quantification is low (CTDI{sub vol} ? 4 ?Gy), the low dose limit for the CT exam as part of SPECT/CT will be guided by CT image quality requirements for anatomical localization and artifact reduction. A CT technique with higher kVp in combination with lower mAs is recommended when low-dose CT images are used for AC to minimize beam-hardening artifacts.

  19. Imaging and quantitative data acquisition of biological cell walls with Atomic Force Microscopy and Scanning Acoustic Microscopy

    SciTech Connect (OSTI)

    Tittmann, B. R.; Xi, X.

    2014-09-01

    This chapter demonstrates the feasibility of Atomic Force Microscopy (AFM) and High Frequency Scanning Acoustic Microscopy (HF-SAM) as tools to characterize biological tissues. Both the AFM and the SAM have shown to provide imaging (with different resolution) and quantitative elasticity measuring abilities. Plant cell walls with minimal disturbance and under conditions of their native state have been examined with these two kinds of microscopy. After descriptions of both the SAM and AFM, their special features and the typical sample preparation is discussed. The sample preparation is focused here on epidermal peels of onion scales and celery epidermis cells which were sectioned for the AFM to visualize the inner surface (closest to the plasma membrane) of the outer epidermal wall. The nm-wide cellulose microfibrils orientation and multilayer structure were clearly observed. The microfibril orientation and alignment tend to be more organized in older scales compared with younger scales. The onion epidermis cell wall was also used as a test analog to study cell wall elasticity by the AFM nanoindentation and the SAM V(z) feature. The novelty in this work was to demonstrate the capability of these two techniques to analyze isolated, single layered plant cell walls in their natural state. AFM nanoindentation was also used to probe the effects of Ethylenediaminetetraacetic acid (EDTA), and calcium ion treatment to modify pectin networks in cell walls. The results suggest a significant modulus increase in the calcium ion treatment and a slight decrease in EDTA treatment. To complement the AFM measurements, the HF-SAM was used to obtain the V(z) signatures of the onion epidermis. These measurements were focused on documenting the effect of pectinase enzyme treatment. The results indicate a significant change in the V(z) signature curves with time into the enzyme treatment. Thus AFM and HF-SAM open the door to a systematic nondestructive structure and mechanical property study of complex biological cell walls. A unique feature of this approach is that both microscopes allow the biological samples to be examined in their natural fluid (water) environment.

  20. Quantitative modeling of the accuracy in registering preoperative patient-specific anatomic models into left atrial cardiac ablation procedures

    SciTech Connect (OSTI)

    Rettmann, Maryam E. Holmes, David R.; Camp, Jon J.; Cameron, Bruce M.; Robb, Richard A.; Kwartowitz, David M.; Gunawan, Mia; Johnson, Susan B.; Packer, Douglas L.; Dalegrave, Charles; Kolasa, Mark W.

    2014-02-15

    Purpose: In cardiac ablation therapy, accurate anatomic guidance is necessary to create effective tissue lesions for elimination of left atrial fibrillation. While fluoroscopy, ultrasound, and electroanatomic maps are important guidance tools, they lack information regarding detailed patient anatomy which can be obtained from high resolution imaging techniques. For this reason, there has been significant effort in incorporating detailed, patient-specific models generated from preoperative imaging datasets into the procedure. Both clinical and animal studies have investigated registration and targeting accuracy when using preoperative models; however, the effect of various error sources on registration accuracy has not been quantitatively evaluated. Methods: Data from phantom, canine, and patient studies are used to model and evaluate registration accuracy. In the phantom studies, data are collected using a magnetically tracked catheter on a static phantom model. Monte Carlo simulation studies were run to evaluate both baseline errors as well as the effect of different sources of error that would be present in a dynamicin vivo setting. Error is simulated by varying the variance parameters on the landmark fiducial, physical target, and surface point locations in the phantom simulation studies. In vivo validation studies were undertaken in six canines in which metal clips were placed in the left atrium to serve as ground truth points. A small clinical evaluation was completed in three patients. Landmark-based and combined landmark and surface-based registration algorithms were evaluated in all studies. In the phantom and canine studies, both target registration error and point-to-surface error are used to assess accuracy. In the patient studies, no ground truth is available and registration accuracy is quantified using point-to-surface error only. Results: The phantom simulation studies demonstrated that combined landmark and surface-based registration improved landmark-only registration provided the noise in the surface points is not excessively high. Increased variability on the landmark fiducials resulted in increased registration errors; however, refinement of the initial landmark registration by the surface-based algorithm can compensate for small initial misalignments. The surface-based registration algorithm is quite robust to noise on the surface points and continues to improve landmark registration even at high levels of noise on the surface points. Both the canine and patient studies also demonstrate that combined landmark and surface registration has lower errors than landmark registration alone. Conclusions: In this work, we describe a model for evaluating the impact of noise variability on the input parameters of a registration algorithm in the context of cardiac ablation therapy. The model can be used to predict both registration error as well as assess which inputs have the largest effect on registration accuracy.

  1. Assessment of beating parameters in human induced pluripotent stem cells enables quantitative in vitro screening for cardiotoxicity

    SciTech Connect (OSTI)

    Sirenko, Oksana; Cromwell, Evan F.; Crittenden, Carole; Wignall, Jessica A.; Wright, Fred A.; Rusyn, Ivan

    2013-12-15

    Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes show promise for screening during early drug development. Here, we tested a hypothesis that in vitro assessment of multiple cardiomyocyte physiological parameters enables predictive and mechanistically-interpretable evaluation of cardiotoxicity in a high-throughput format. Human iPSC-derived cardiomyocytes were exposed for 30 min or 24 h to 131 drugs, positive (107) and negative (24) for in vivo cardiotoxicity, in up to 6 concentrations (3 nM to 30 uM) in 384-well plates. Fast kinetic imaging was used to monitor changes in cardiomyocyte function using intracellular Ca{sup 2+} flux readouts synchronous with beating, and cell viability. A number of physiological parameters of cardiomyocyte beating, such as beat rate, peak shape (amplitude, width, raise, decay, etc.) and regularity were collected using automated data analysis. Concentration–response profiles were evaluated using logistic modeling to derive a benchmark concentration (BMC) point-of-departure value, based on one standard deviation departure from the estimated baseline in vehicle (0.3% dimethyl sulfoxide)-treated cells. BMC values were used for cardiotoxicity classification and ranking of compounds. Beat rate and several peak shape parameters were found to be good predictors, while cell viability had poor classification accuracy. In addition, we applied the Toxicological Prioritization Index (ToxPi) approach to integrate and display data across many collected parameters, to derive “cardiosafety” ranking of tested compounds. Multi-parameter screening of beating profiles allows for cardiotoxicity risk assessment and identification of specific patterns defining mechanism-specific effects. These data and analysis methods may be used widely for compound screening and early safety evaluation in drug development. - Highlights: • Induced pluripotent stem cell-derived cardiomyocytes are promising in vitro models. • We tested if evaluation of cardiotoxicity is possible in a high-throughput format. • The assay shows benefits of automated data integration across multiple parameters. • Quantitative assessment of concentration–response is possible using iPSCs. • Multi-parametric screening allows for cardiotoxicity risk assessment.

  2. Textures in Strip-Cast Aluminum Alloys: Their On-Line Monitoring and Quantitative Effects on Formability. Final Technical Report

    SciTech Connect (OSTI)

    Man, Chi-Sing

    2003-07-27

    Aluminum sheets produced by continuous casting (CC) provide energy and economic savings of at least 25 and 14 percent, respectively, over sheets made from conventional direct chill (DC) ingot casting and rolling. As a result of the much simpler production route in continuous casting, however, the formability of CC aluminum alloys is often somewhat inferior to that of their DC counterparts. The mechanical properties of CC alloys can be improved by controlling their microstructure through optimal thermomechanical processing. Suitable annealing is an important means to improve the formability of CC aluminum alloy sheets. Recrystallization of deformed grains occurs during annealing, and it changes the crystallographic texture of the aluminum sheet. Laboratory tests in this project showed that this texture change can be detected by either laser-ultrasound resonance spectroscopy or resonance EMAT (electromagnetic acoustic transducer) spectroscopy, and that monitoring this change allows the degree of recrystallization or the ''recrystallized fraction'' in an annealed sheet to be ascertained. Through a plant trial conducted in May 2002, this project further demonstrated that it is feasible to monitor the recrystallized state of a continuous-cast aluminum sheet in-situ on the production line by using a laser-ultrasound sensor. When used in conjunction with inline annealing, inline monitoring of the recrystallized fraction by laser-ultrasound resonance spectroscopy offers the possibility of feed-back control that helps optimize processing parameters (e.g., annealing temperature), detect production anomalies, ensure product quality, and further reduce production costs of continuous-cast aluminum alloys. Crystallographic texture strongly affects the mechanical anisotropy/formability of metallic sheets. Clarification of the quantitative relationship between texture and anisotropy/formability of an aluminum alloy will render monitoring and control of its texture during the sheet production process even more meaningful. The present project included a study to determine how the anisotropic plastic behavior of a continuous-cast AA 5754 aluminum alloy depends on quantifiable texture coefficients. Formulae which show explicitly the effects of texture on the directional dependence of the q-value (a formability parameter) and of the uniaxial flow stress, respectively, were derived. Measurements made on a batch of as-received AA 5754 hot band and its O-temper counterpart corroborate the validity of these formulae. On the other hand, these measurements also indicate that some microstructure(s) other than texture could play a significant role in the plastic anisotropy of the AA 5754 alloy. For the q-value of a set of O-temper samples of this alloy, the additional microstructure that affects plastic anisotropy was shown to be grain shape. A formula that captures both the effects of crystallographic texture and grain shape on the q-value of the O-temper material was derived. A simple quadratic plastic potential that delivers this q-value formula was written down. Verification of the adequacy of this plastic potential, however, requires further investigations.

  3. An elusive species with many different traits.

    E-Print Network [OSTI]

    Giesen, Thomas

    the astronomer, where he lectured with much applause in the lecture-room, How soon, unaccountable, I became tired-Pase detection of HSOH: Synthesis by Flash Vacuum Pyrolysis of ditert-butyl-sulfoxide and rotational- torsional

  4. Inheritance of Black Pericarp Trait in Sorghum 

    E-Print Network [OSTI]

    Hill, Kathleen Marie

    2014-09-18

    high and low pH levels, making it a viable candidate for use as a natural food colorant and preservative (Pfeiffer, 2014). Sorghum is also naturally gluten free (Pfeiffer, 2014) making it an excellent wheat substitute for those who suffer from Celiac... as antioxidants (Dlamini et al., 2007) and in the body, ward off free radicals that lead to disease (Rajendran, et. al., 2014). Sorghum, 5 compared to other cereals, has the highest level of antioxidants (Pfeiffer, 2014). In fact, black sorghum has levels...

  5. Arbuscular mycorrhizal interactions ? an important trait for...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Determination Symbiosis Conference Speaker and Attendee List Symbiosis Biofeedstock Conference: Expanding Commercialization of Mutualistic Microbes to Increase Feedstock Production...

  6. APOT : automatic path optimizing for traits

    E-Print Network [OSTI]

    Lin, Thomas, 1981-

    2004-01-01

    This thesis considers the design of automated tutoring systems that customize teaching material to accommodate individual student learning styles. In particular, we consider the following problem: Begin with one or more ...

  7. A novel approach to the identification and quantitative elemental analysis of amyloid deposits-Insights into the pathology of Alzheimer's disease

    SciTech Connect (OSTI)

    Rajendran, Reshmi; Minqin, Ren [Centre for Ion Beam Applications, Department of Physics, Blk S 12, Faculty of Science, National University of Singapore, 2, Science Drive 3, Singapore 117542 (Singapore)] [Centre for Ion Beam Applications, Department of Physics, Blk S 12, Faculty of Science, National University of Singapore, 2, Science Drive 3, Singapore 117542 (Singapore); Ynsa, Maria Dolores [Centro de Micro-Analisis de Materiales (CMAM), Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid (Spain)] [Centro de Micro-Analisis de Materiales (CMAM), Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid (Spain); Casadesus, Gemma [Department of Neurosciences, Case Western Reserve University, Cleveland, OH 44106 (United States)] [Department of Neurosciences, Case Western Reserve University, Cleveland, OH 44106 (United States); Smith, Mark A.; Perry, George [Department of Pathology, Case Western Reserve University, Cleveland, OH 44106 (United States)] [Department of Pathology, Case Western Reserve University, Cleveland, OH 44106 (United States); Halliwell, Barry [Department of Biochemistry, National University of Singapore (Singapore)] [Department of Biochemistry, National University of Singapore (Singapore); Watt, Frank, E-mail: phywattf@nus.edu.sg [Centre for Ion Beam Applications, Department of Physics, Blk S 12, Faculty of Science, National University of Singapore, 2, Science Drive 3, Singapore 117542 (Singapore)] [Centre for Ion Beam Applications, Department of Physics, Blk S 12, Faculty of Science, National University of Singapore, 2, Science Drive 3, Singapore 117542 (Singapore)

    2009-04-24

    There is considerable interest in the role of metals such as iron, copper, and zinc in amyloid plaque formation in Alzheimer's disease. However to convincingly establish their presence in plaques in vivo, a sensitive technique is required that is both quantitatively accurate and avoids isolation of plaques or staining/fixing brain tissue, since these processes introduce contaminants and redistribute elements within the tissue. Combining the three ion beam techniques of scanning transmission ion microscopy, Rutherford back scattering spectrometry and particle induced X-ray emission in conjunction with a high energy (MeV) proton microprobe we have imaged plaques in freeze-dried unstained brain sections from CRND-8 mice, and simultaneously quantified iron, copper, and zinc. Our results show increased metal concentrations within the amyloid plaques compared with the surrounding tissue: iron (85 ppm compared with 42 ppm), copper (16 ppm compared to 6 ppm), and zinc (87 ppm compared to 34 ppm).

  8. A Quantitative Model of Energy Release and Heating by Time-dependent, Localized Reconnection in a Flare with a Thermal Loop-top X-ray Source

    E-Print Network [OSTI]

    Longcope, D W; Carranza-Fulmer, T; Qiu, J; 10.1007/s11207-010-9635-z

    2011-01-01

    We present a quantitative model of the magnetic energy stored and then released through magnetic reconnection for a flare on 26 Feb 2004. This flare, well observed by RHESSI and TRACE, shows evidence of non-thermal electrons only for a brief, early phase. Throughout the main period of energy release there is a super-hot (T>30 MK) plasma emitting thermal bremsstrahlung atop the flare loops. Our model describes the heating and compression of such a source by localized, transient magnetic reconnection. It is a three-dimensional generalization of the Petschek model whereby Alfven-speed retraction following reconnection drives supersonic inflows parallel to the field lines, which form shocks heating, compressing, and confining a loop-top plasma plug. The confining inflows provide longer life than a freely-expanding or conductively-cooling plasma of similar size and temperature. Superposition of successive transient episodes of localized reconnection across a current sheet produces an apparently persistent, localiz...

  9. Method for quantitative determination and separation of trace amounts of chemical elements in the presence of large quantities of other elements having the same atomic mass

    DOE Patents [OSTI]

    Miller, C.M.; Nogar, N.S.

    1982-09-02

    Photoionization via autoionizing atomic levels combined with conventional mass spectroscopy provides a technique for quantitative analysis of trace quantities of chemical elements in the presence of much larger amounts of other elements with substantially the same atomic mass. Ytterbium samples smaller than 10 ng have been detected using an ArF* excimer laser which provides the atomic ions for a time-of-flight mass spectrometer. Elemental selectivity of greater than 5:1 with respect to lutetium impurity has been obtained. Autoionization via a single photon process permits greater photon utilization efficiency because of its greater absorption cross section than bound-free transitions, while maintaining sufficient spectroscopic structure to allow significant photoionization selectivity between different atomic species. Separation of atomic species from others of substantially the same atomic mass is also described.

  10. Quantitative assessment of alkali-reactive aggregate mineral content through XRD using polished sections as a supplementary tool to RILEM AAR-1 (petrographic method)

    SciTech Connect (OSTI)

    Castro, Nelia; Sorensen, Bjorn E.; Broekmans, Maarten A.T.M.

    2012-11-15

    The mineral content of 5 aggregate samples from 4 different countries, including reactive and non-reactive aggregate types, was assessed quantitatively by X-ray diffraction (XRD) using polished sections. Additionally, electron probe microanalyzer (EPMA) mapping and cathodoluminescence (CL) were used to characterize the opal-CT identified in one of the aggregate samples. Critical review of results from polished sections against traditionally powdered specimen has demonstrated that for fine-grained rocks without preferred orientation the assessment of mineral content by XRD using polished sections may represent an advantage over traditional powder specimens. Comparison of data on mineral content and silica speciation with expansion data from PARTNER project confirmed that the presence of opal-CT plays an important role in the reactivity of one of the studied aggregates. Used as a complementary tool to RILEM AAR-1, the methodology suggested in this paper has the potential to improve the strength of the petrographic method.

  11. [Studies of biologic activation associated with molecular receptor increase and tumor response in ChL6/L6 protocol patients; Studies in phantoms; Quantitative SPECT; Preclinical studies; and Clinical studies]. DOE annual report, 1994--95

    SciTech Connect (OSTI)

    DeNardo, S.J.

    1995-12-31

    The authors describe results which have not yet been published from their associated studies listed in the title. For the first, they discuss Lym-1 single chain genetically engineered molecules, analysis of molecular genetic coded messages to enhance tumor response, and human dosimetry and therapeutic human use radiopharmaceuticals. Studies in phantoms includes a discussion of planar image quantitation, counts coincidence correction, organ studies, tumor studies, and {sup 90}Y quantitation with Bremsstrahlung imaging. The study on SPECT discusses attenuation correction and scatter correction. Preclinical studies investigated uptake of {sup 90}Y-BrE-3 in mice using autoradiography. Clinical studies discuss image quantitation verses counts from biopsy samples, S factors for radiation dose calculation, {sup 67}Cu imaging studies for lymphoma cancer, and {sup 111}In MoAb imaging studies for breast cancer to predict {sup 90}Y MoAb therapy.

  12. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z; Wan, Kai-tak

    2014-09-02

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  13. SU-E-J-229: Quantitative Assessment for Timely Adaptive Re-Planning Using Weekly Dose Monitoring for Head and Neck Cancer

    SciTech Connect (OSTI)

    Shang, Q; Liu, H; Greskovich, J; Koyfman, S; Xia, P; Li, Z

    2014-06-01

    Purpose: For patients with head and neck (HN) cancer, mid-course adaptive radiation therapy (ART) is a common practice in our institution to accommodate anatomic changes. The aim of the study is to evaluate whether dose re-calculation on weekly verification images can provide quantitative assessment for timely adaptive re-planning with daily image-guided intensity modulated radiotherapy (IMRT). Methods: We retrospectively selected sixty daily verification images acquired on CT-on-rail/CBCT from ten HN patients. These image sets were typically a week apart. Among these patients, six patients received a mid-course ART. Contours of the tumors and organ-at-risks (OARs) were manually delineated by a physician on each verification CT. After placing the treatment iso-center on the verification CTs according to the recorded clinical shifts, daily dose was re-calculated with the same beam configuration as the original plan. For the purpose of this study, electron densities for both verification CTs and planning CTs were set to 1.0 g/cm3. Results: Two patients had D99 of the CTV < 97% of the planned dose for more than three fractions due to remarkable tumor volume shrinkages. D-max of the spinal cord exceeded a tolerance of 45 Gy for four fractions in additional two patients. D-mean of the parotid increased within 25% of the planned value. D-max of the brainstem and D-mean of the oral cavity did not show significant variation. If the re-planning criteria included D99 of the CTV < 97% of the planned dose and D-max of the spinal cord > 45 Gy, two out ten patients required ART at week 2 and two patients required ART at week 3, respectively. Conclusion: Weekly dose monitoring with re-calculation on verification images can provide quantitative dose guidance for timely adaptive re-planning. Future work will include accumulative dose analysis for the decision of adaptive re-planning. The study is supported in part by Siemens Medical Solutions.

  14. A quantitative model of trading and price formation in nancial markets Marcus G. Daniels, 1 J. Doyne Farmer, 1 L aszl o Gillemot, 1 Giulia Iori, 2 and Eric Smith 1

    E-Print Network [OSTI]

    A quantitative model of trading and price formation in #12;nancial markets Marcus G. Daniels, 1 J y (Dated: January 29, 2002) We use standard physics techniques to model trading and price formation of prices, which is the standard measure of #12;nancial risk, and the spread and price impact functions

  15. Page 274 Courses: Chemistry (CHEM) Sonoma State University 2015-2016 Catalog CHeM 125A QuAntitAtive generAL CHeMiStry (5)

    E-Print Network [OSTI]

    Ravikumar, B.

    Page 274 Courses: Chemistry (CHEM) Sonoma State University 2015-2016 Catalog CHeM 125A QuAntitAtive generAL CHeMiStry (5) Lecture, 3 hours; discussion 1 hour; laboratory 3 hours (5 units). This one-year analytical general chemistry course is designed for freshmen Chemistry majors, Biochemistry majors, or others

  16. Departures from local thermodynamic equilibrium in cutting arc plasmas derived from electron and gas density measurements using a two-wavelength quantitative Schlieren technique

    SciTech Connect (OSTI)

    Prevosto, L.; Mancinelli, B.; Artana, G.; Kelly, H.

    2011-03-15

    A two-wavelength quantitative Schlieren technique that allows inferring the electron and gas densities of axisymmetric arc plasmas without imposing any assumption regarding statistical equilibrium models is reported. This technique was applied to the study of local thermodynamic equilibrium (LTE) departures within the core of a 30 A high-energy density cutting arc. In order to derive the electron and heavy particle temperatures from the inferred density profiles, a generalized two-temperature Saha equation together with the plasma equation of state and the quasineutrality condition were employed. Factors such as arc fluctuations that influence the accuracy of the measurements and the validity of the assumptions used to derive the plasma species temperature were considered. Significant deviations from chemical equilibrium as well as kinetic equilibrium were found at elevated electron temperatures and gas densities toward the arc core edge. An electron temperature profile nearly constant through the arc core with a value of about 14000-15000 K, well decoupled from the heavy particle temperature of about 1500 K at the arc core edge, was inferred.

  17. Investigation of EMIC wave scattering as the cause for the BARREL 17 January 2013 relativistic electron precipitation event: A quantitative comparison of simulation with observations

    SciTech Connect (OSTI)

    Li, Zan; Millan, Robyn M.; Hudson, Mary K.; Woodger, Leslie A.; Smith, David M.; Chen, Yue; Friedel, Reiner; Rodriguez, Juan V.; Engebretson, Mark J.; Goldstein, Jerry; Fennell, Joseph F.; Spence, Harlan E.

    2014-12-23

    Electromagnetic ion cyclotron (EMIC) waves were observed at multiple observatory locations for several hours on 17 January 2013. During the wave activity period, a duskside relativistic electron precipitation (REP) event was observed by one of the Balloon Array for Radiation belt Relativistic Electron Losses (BARREL) balloons and was magnetically mapped close to Geostationary Operational Environmental Satellite (GOES) 13. We simulate the relativistic electron pitch angle diffusion caused by gyroresonant interactions with EMIC waves using wave and particle data measured by multiple instruments on board GOES 13 and the Van Allen Probes. We show that the count rate, the energy distribution, and the time variation of the simulated precipitation all agree very well with the balloon observations, suggesting that EMIC wave scattering was likely the cause for the precipitation event. The event reported here is the first balloon REP event with closely conjugate EMIC wave observations, and our study employs the most detailed quantitative analysis on the link of EMIC waves with observed REP to date.

  18. Investigation of EMIC wave scattering as the cause for the BARREL 17 January 2013 relativistic electron precipitation event: A quantitative comparison of simulation with observations

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Zan; Millan, Robyn M.; Hudson, Mary K.; Woodger, Leslie A.; Smith, David M.; Chen, Yue; Friedel, Reiner; Rodriguez, Juan V.; Engebretson, Mark J.; Goldstein, Jerry; et al

    2014-12-23

    Electromagnetic ion cyclotron (EMIC) waves were observed at multiple observatory locations for several hours on 17 January 2013. During the wave activity period, a duskside relativistic electron precipitation (REP) event was observed by one of the Balloon Array for Radiation belt Relativistic Electron Losses (BARREL) balloons and was magnetically mapped close to Geostationary Operational Environmental Satellite (GOES) 13. We simulate the relativistic electron pitch angle diffusion caused by gyroresonant interactions with EMIC waves using wave and particle data measured by multiple instruments on board GOES 13 and the Van Allen Probes. We show that the count rate, the energy distribution,more »and the time variation of the simulated precipitation all agree very well with the balloon observations, suggesting that EMIC wave scattering was likely the cause for the precipitation event. The event reported here is the first balloon REP event with closely conjugate EMIC wave observations, and our study employs the most detailed quantitative analysis on the link of EMIC waves with observed REP to date.« less

  19. Homogeneity testing and quantitative analysis of manganese (Mn) in vitrified Mn-doped glasses by laser-induced breakdown spectroscopy (LIBS)

    SciTech Connect (OSTI)

    Unnikrishnan, V. K.; Nayak, Rajesh; Kartha, V. B.; Santhosh, C. E-mail: unnikrishnan.vk@manipal.edu; Sonavane, M. S.; Yeotikar, R. G.; Shah, M. L.; Gupta, G. P.; Suri, B. M.

    2014-09-15

    Laser-induced breakdown spectroscopy (LIBS), an atomic emission spectroscopy method, has rapidly grown as one of the best elemental analysis techniques over the past two decades. Homogeneity testing and quantitative analysis of manganese (Mn) in manganese-doped glasses have been carried out using an optimized LIBS system employing a nanosecond ultraviolet Nd:YAG laser as the source of excitation. The glass samples have been prepared using conventional vitrification methods. The laser pulse irradiance on the surface of the glass samples placed in air at atmospheric pressure was about 1.7×10{sup 9} W/cm{sup 2}. The spatially integrated plasma emission was collected and imaged on to the spectrograph slit using an optical-fiber-based collection system. Homogeneity was checked by recording LIBS spectra from different sites on the sample surface and analyzing the elemental emission intensities for concentration determination. Validation of the observed LIBS results was done by comparison with scanning electron microscope- energy dispersive X-ray spectroscopy (SEM-EDX) surface elemental mapping. The analytical performance of the LIBS system has been evaluated through the correlation of the LIBS determined concentrations of Mn with its certified values. The results are found to be in very good agreement with the certified concentrations.

  20. Quantitative characterization of the protein contents of the exocrine pancreatic acinar cell by soft x-ray microscopy and advanced digital imaging methods

    SciTech Connect (OSTI)

    Loo Jr., Billy W.

    2000-06-09

    The study of the exocrine pancreatic acinar cell has been central to the development of models of many cellular processes, especially of protein transport and secretion. Traditional methods used to examine this system have provided a wealth of qualitative information from which mechanistic models have been inferred. However they have lacked the ability to make quantitative measurements, particularly of the distribution of protein in the cell, information critical for grounding of models in terms of magnitude and relative significance. This dissertation describes the development and application of new tools that were used to measure the protein content of the major intracellular compartments in the acinar cell, particularly the zymogen granule. Soft x-ray microscopy permits image formation with high resolution and contrast determined by the underlying protein content of tissue rather than staining avidity. A sample preparation method compatible with x-ray microscopy was developed and its properties evaluated. Automatic computerized methods were developed to acquire, calibrate, and analyze large volumes of x-ray microscopic images of exocrine pancreatic tissue sections. Statistics were compiled on the protein density of several organelles, and on the protein density, size, and spatial distribution of tens of thousands of zymogen granules. The results of these measurements, and how they compare to predictions of different models of protein transport, are discussed.