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Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

E-Print Network 3.0 - avian flu virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Ecology 15 prepared by the Pandemic Communications Committee Summary: or progressive cough Understand 12;What is avian flu? An illness caused by influenza viruses occurring...

2

A Guide For Parents FLU INFORMATION  

E-Print Network [OSTI]

viruses spread mainly by droplets made when people with flu cough, sneeze or talk. These droplets can land of the flu? Symptoms of flu can include fever, cough, sore throat, runny or stuffy nose, body aches, headache

Burke, Peter

3

Avian Flu  

SciTech Connect (OSTI)

Since 2003, a severe form of H5N1 avian influenza has rapidly spread throughout Asia and Europe, infecting over 200 humans in 10 countries. The spread of H5N1 virus from person-to-person has been rare, thus preventing the emergence of a widespread pandemic. However, this ongoing epidemic continues to pose an important public health threat. Avian flu and its pandemic potential in humans will be discussed.

Eckburg, Paul

2006-11-06T23:59:59.000Z

4

E-Print Network 3.0 - avian flu update Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

is seasonal flu? Happens every year between November and March... or progressive cough Understand 12;What is avian flu? An illness caused by influenza viruses occurring...

5

E-Print Network 3.0 - attacks avian flu Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

is seasonal flu? Happens every year between November and March... or progressive cough Understand 12;What is avian flu? An illness caused by influenza viruses occurring...

6

Everyday Preventive Actions That Can Help Fight Germs, Like Flu  

E-Print Network [OSTI]

spread? Flu viruses are thought to spread mainly from person to person through the coughing, sneezing and mouth with a tissuey when you cough or sneeze. This will block the spread of droplets from your mouth

Tipple, Brett

7

Swine Influenza (swine flu) Fact Sheet What is swine flu?  

E-Print Network [OSTI]

that the child does not want to be held · Flu-like symptoms improve but then return with fever and worse cough your health: #12;· Cover your nose and mouth with a tissue when you cough or sneeze. Throw the tissue in the trash after you use it. · Wash your hands often with soap and water, especially after you cough

Olsen, Stephen L.

8

Nucleoprotein of influenza B virus binds to its type A counterpart and disrupts influenza A viral polymerase complex formation  

SciTech Connect (OSTI)

Highlights: FluB nucleoprotein (BNP) can bind to FluA nucleoprotein (ANP). BNPANP interaction inhibits FluA polymerase activity. BNP binding prevents ANP from forming a functional FluA polymerase complex. Nuclear localization of BNP is necessary for FluA polymerase inhibition. Viral RNA is not required for the BNPANP interaction. -- Abstract: Upon co-infection with influenza B virus (FluB), influenza A virus (FluA) replication is substantially impaired. Previously, we have shown that the nucleoprotein of FluB (BNP) can inhibit FluA polymerase machinery, retarding the growth of FluA. However, the molecular mechanism underlying this inhibitory action awaited further investigation. Here, we provide evidence that BNP hinders the proper formation of FluA polymerase complex by competitively binding to the nucleoprotein of FluA. To exert this inhibitory effect, BNP must be localized in the nucleus. The interaction does not require the presence of the viral RNA but needs an intact BNP RNA-binding motif. The results highlight the novel role of BNP as an anti-influenza A viral agent and provide insights into the mechanism of intertypic interference.

Jaru-ampornpan, Peera, E-mail: peera.jar@biotec.or.th; Narkpuk, Jaraspim; Wanitchang, Asawin; Jongkaewwattana, Anan, E-mail: anan.jon@biotec.or.th

2014-01-03T23:59:59.000Z

9

FLU ALERT SYMPTOMS AND TREATMENT Spring 2014  

E-Print Network [OSTI]

: Dry Cough Nasal Congestion Sore Throat THE BEST TREATMENT FOR THE FLU IS ­ Rest! Cough syrup if coughing interferes with sleep You are most contagious from the day before your symptoms begin, through the period of prominent symptoms. Symptoms usually last a week, but coughing can persist

Rhode Island, University of

10

INFLUENZA 101 Symptoms of the flu  

E-Print Network [OSTI]

INFLUENZA 101 Symptoms of the flu Sudden onset of fever/chills, coughing, muscle aches, headache · Stay home and limit contact with others until coughing and/or sneezing subside (usually · If having difficulty breathing doing ordinary things, or are coughing up blood · If not improving by the end

Abolmaesumi, Purang

11

Pandemic Flu: What To Know and  

E-Print Network [OSTI]

cough or sneeze Throw the tissue in the trash after you use it Ifor sneeze. Throw the tissue in the trash after you use it. If a tissue is unavailable, cough or sneeze into your shoulder or elbow instead sick children at home. ­ If you have flu-like symptoms (fever with cough or sore throat), stay home

Tipple, Brett

12

September 2007 Influenza (Flu) Immunization: Myths and Facts  

E-Print Network [OSTI]

, or flu shot, is the best protection against illness and complications. Many people use the term the `flu serious illness and even death for people with certain chronic health concerns. The influenza vaccine in BC, hundreds of people die from influenza or complications, such as pneumonia. Influenza in people 65

13

PREPARE NOW TO BEAT THE FLU! (2010-2011) GET THE FLU VACCINE AS SOON AS POSSIBLE,  

E-Print Network [OSTI]

is ok) 5. Cough syrup (similar to Robitussin DM, generic is ok) 6. Tissues Students can purchase a "Flu, hand sanitizer, acetaminophen, cough syrup, and throat lozenges. You may also purchase the items above, cough, sore throat, and body aches. · Most individuals who get the flu who do not have a high risk

Saldin, Dilano

14

Dear CSU students, faculty and staff, There has been a lot of attention recently paid to the severity of this flu season and the importance of  

E-Print Network [OSTI]

OF YOURSELF OR SOMEONE ELSE WITH THE FLU: Flu symptoms are fever, cough, sore throat, runny or stuffy nose

Stephens, Graeme L.

15

Foiling the Flu Bug Global Partnerships for Nuclear Energy  

E-Print Network [OSTI]

1 1663 Foiling the Flu Bug Global Partnerships for Nuclear Energy Dark Universe Mysteries WILL NOT NEED TESTING Expanding Nuclear Energy the Right Way GLOBAL PARTNERSHIPS AND AN ADVANCED FUEL CYCLE sense.The Laboratory is operated by Los Alamos National Security, LLC, for the Department of Energy

16

EDUCATION BULLETIN INFLUENZA A (H1N1) VIRUS  

E-Print Network [OSTI]

and respiratory etiquette. These measures are also effective in curtailing the spread of the seasonal flu virus through the MELS Internet application. If the situation were to change, however, this mechanism could of this recommendation will have on pregnant school system employees who are currently on preventive leave because

17

E-Print Network 3.0 - avian flu vaccine Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

is seasonal flu? Happens every year between November and March... or progressive cough Understand ... Source: Stowell, Michael - Department of Molecular, Cellular, and...

18

Structure and Receptor Specificity of an Avian Flu Antigen  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

a limited ability to infect humans. However, with continued outbreaks of the virus in poultry and wild birds, the potential for the emergence of a human-adapted H5 virus, either...

19

What can you do to protect yourself and others against Flu?  

E-Print Network [OSTI]

respiratory and hand hygiene: Always carry tissues. Cover your nose and mouth when coughing or sneezing Lack of appetite o Coughing o Sore throat o Some people with swine flu have also reported having

Davies, Christopher

20

City of Chicago won't sweat the flu with Argonne's help | Argonne...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

track city agencies' responses. After carefully planning out the spread of the "flu attack," they launched the scenario. Over a three-day period, at specific points, they fed...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Students experiencing flu-like symptoms, such as fever with cough and/or sore throat,  

E-Print Network [OSTI]

Students experiencing flu-like symptoms, such as fever with cough and/or sore throat, should other difficulty breathing beyond a typical cough. Inform Undergraduates: Notify the director

Bou-Zeid, Elie

22

What can you do to stop the flu? Cover your cough! Help stop the spread of germs by  

E-Print Network [OSTI]

What can you do to stop the flu? · Cover your cough! Help stop the spread of germs by covering your mouth and nose when you cough or sneeze with a tissue or with your upper sleeve. · Wash your hands surfaces. · Flu symptoms include: - Fever - Extreme tiredness - Headache - Cough - Muscle aches - Runny

Burke, Peter

23

HelenPilcher,London An avian flu virus is mutating and  

E-Print Network [OSTI]

an unspecified period of time through the auction of radio spectrum that is currently used for television broad that, if elected, he would lift Pre

Cai, Long

24

E-Print Network 3.0 - african swine fever virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Flu" (H1N1 Influenza) Similarities to seasonal flu H1N1 flu symptoms - Fever, cough, muscle... Swine Flu and Common Infections to Prepare For Rochester ... Source:...

25

Real-Time Disease Surveillance Using Twitter Data: Demonstration on Flu and Cancer  

E-Print Network [OSTI]

, and text mining. The real-time analysis data is visually re- ported as U.S. disease activity map, timelinesReal-Time Disease Surveillance Using Twitter Data: Demonstration on Flu and Cancer Kathy Lee Ankit-effects, and so on. This makes publicly available social media data an invaluable resource for mining interesting

26

What are the symptoms of flu? Symptoms include sudden onset of  

E-Print Network [OSTI]

What are the symptoms of flu? · Symptoms include sudden onset of: o fever/chills o cough o muscle lasting 5 or more days (measured with a thermometer; 37.0 Celsius is normal) · Coughing up blood that improve but then return with fever and worse cough Those in high risk groups including pregnant women

Ellis, Randy

27

Cover your Cough! Quantifying the Benefits of a Localized Healthy Behavior Intervention on Flu  

E-Print Network [OSTI]

Cover your Cough! Quantifying the Benefits of a Localized Healthy Behavior Intervention on Flu a policy that encourages healthy behaviors (such as covering your cough and using hand sanitizers) at four coughs, minimizing contact with potential fomites) at major tourist locations. We use a synthetic

Swarup, Samarth

28

Worried about H1N1 SWINE FLU? What you should do.  

E-Print Network [OSTI]

with severe illness like difficulty breathing AND YOU HAVE Fever (100.4) plus cough or sore throat Fever (100.4) plus cough or sore throat Fever (100.4) plus cough or sore throat YOU SHOULD Stay home until you fever, cough, sore throat, tiredness, aches, chills and stuffy nose. H1N1 flu spreads when a sick person

Tipple, Brett

29

PREPARE NOW TO BEAT THE FLU! Updated for 2012-2013 Influenza Season  

E-Print Network [OSTI]

is ok) 5. Cough syrup (similar to Robitussin DM, generic is ok) 6. Tissues 7. Contact numbers: Primary sanitizer, acetaminophen, cough syrup, and throat lozenges. These are also located at the Sandburg Emporium include: Fever over 100 degrees F, cough, sore throat, and body aches. · Most individuals who get the flu

Saldin, Dilano

30

Worried about H1N1 SWINE FLU? What you should do.  

E-Print Network [OSTI]

with severe illness like difficulty breathing AND YOU HAVE Fever (100.4) plus cough or sore throat Fever (100.4) plus cough or sore throat Fever (100.4) plus cough or sore throat YOU SHOULD Stay home until you. The illness can cause fever, cough, sore throat, tiredness, aches, chills and stuffy nose. H1N1 flu spreads

Brinkmann, Peter

31

FluCaP: A Heuristic Search Planner for First-Order MDPs  

E-Print Network [OSTI]

We present a heuristic search algorithm for solving first-order Markov Decision Processes (FOMDPs). Our approach combines first-order state abstraction that avoids evaluating states individually, and heuristic search that avoids evaluating all states. Firstly, in contrast to existing systems, which start with propositionalizing the FOMDP and then perform state abstraction on its propositionalized version we apply state abstraction directly on the FOMDP avoiding propositionalization. This kind of abstraction is referred to as first-order state abstraction. Secondly, guided by an admissible heuristic, the search is restricted to those states that are reachable from the initial state. We demonstrate the usefulness of the above techniques for solving FOMDPs with a system, referred to as FluCaP (formerly, FCPlanner), that entered the probabilistic track of the 2004 International Planning Competition (IPC2004) and demonstrated an advantage over other planners on the problems represented in first-order terms.

Hoelldobler, S; Skvortsova, O; 10.1613/jair.1965

2011-01-01T23:59:59.000Z

32

Nowcasting economic and social data: when and why search engine data fails, an illustration using Google Flu Trends  

E-Print Network [OSTI]

Obtaining an accurate picture of the current state of the economy is particularly important to central banks and finance ministries, and of epidemics to health ministries. There is increasing interest in the use of search engine data to provide such 'nowcasts' of social and economic indicators. However, people may search for a phrase because they independently want the information, or they may search simply because many others are searching for it. We consider the effect of the motivation for searching on the accuracy of forecasts made using search engine data of contemporaneous social and economic indicators. We illustrate the implications for forecasting accuracy using four episodes in which Google Flu Trends data gave accurate predictions of actual flu cases, and four in which the search data over-predicted considerably. Using a standard statistical methodology, the Bass diffusion model, we show that the independent search for information motive was much stronger in the cases of accurate prediction than in...

Ormerod, Paul; Bentley, R Alexander

2014-01-01T23:59:59.000Z

33

INFLUENZAVACCINE 2009 H1N1 influenza (also called Swine Flu) is caused  

E-Print Network [OSTI]

to person through coughing, sneezing, and sometimes through touching objects contaminated with the virus. Signs of 2009 H1N1 can include: · Fatigue ·Fever ·Sore Throat · Muscle Aches · Chills ·Coughing

Suzuki, Masatsugu

34

INFLUENZAVACCINE 2009 H1N1 influenza (sometimes called Swine Flu) is  

E-Print Network [OSTI]

from person to person through coughing, sneezing, and sometimes through touching objects contaminated with the virus. Signs of 2009 H1N1 can include: · Fatigue ·Fever ·Sore Throat · Muscle Aches · Chills ·Coughing

Suzuki, Masatsugu

35

http://www.swissinfo.org/eng/search/detail/Bird_flu_ban_remains_despite_migration_shift.html?siteSect=881& sid=7420407&cKey=1168586962000  

E-Print Network [OSTI]

, warmer than average temperatures in the Atlantic and Mediterranean ­ all pumping heat into the atmosphere_flu_ban_remains_despite_migration_shift.html?siteSect=881& sid=7420407&cKey=1168586962000 Monday 05.03.2007 Climate warning resonates in Switzerland weather heralded a bumper year for bugs and ticks. He said a cold snap in February or March would still

Alvarez, Nadir

36

arXiv:0706.3330v2[physics.flu-dyn]20Feb2008 Coherent structures in localised and global pipe turbulence  

E-Print Network [OSTI]

arXiv:0706.3330v2[physics.flu-dyn]20Feb2008 Coherent structures in localised and global pipe Walk, Bristol BS8 1TW, United Kingdom The recent discovery of unstable travelling waves (TWs) in pipe in both structure and en- ergy with known TWs using numerical simulations in a long pipe. These only occur

Willis, Ashley

37

The Centers for Disease Control and Prevention (CDC) urges you to take the following steps to protect yourself and others from influenza (the flu)  

E-Print Network [OSTI]

, cough, sore throat, runny or stuffy nose, body aches, headache, chills and fatigue. Some people may also://WWW.FLU.GOV or call 800-CDC-INFO ·Cover your nose and mouth with a tissue when you cough or sneeze. Throw the tissue-LIKE SYMPTOMS INCLUDE: fever cough sorethroat runnyorstuffynose bodyaches headache chills fatigue Some people

Tipple, Brett

38

Generating electricity from viruses  

SciTech Connect (OSTI)

Berkeley Lab's Seung-Wuk Lee discusses "Generating electricity from viruses" in this Oct. 28, 2013 talk, which is part of a Science at the Theater event entitled Eight Big Ideas.

Lee, Seung-Wuk

2013-10-31T23:59:59.000Z

39

Generating electricity from viruses  

ScienceCinema (OSTI)

Berkeley Lab's Seung-Wuk Lee discusses "Generating electricity from viruses" in this Oct. 28, 2013 talk, which is part of a Science at the Theater event entitled Eight Big Ideas.

Lee, Seung-Wuk

2014-06-23T23:59:59.000Z

40

Wheat Streak Mosaic Virus  

E-Print Network [OSTI]

E-337 1/05 Gaylon Morgan, State Extension Small Grains Specialist, College Station, TX Carl Patrick, Extension Entomologist, Amarillo, TX Karl Steddom and Charlie Rush, Plant Pathologists, Amarillo, TX W Overview of WSMV and HPV Wheat Streak Mosaic.... Charlie Rush, Plant Pathologist at TAMU?Amarillo. Figure 4. Wheat field infected with Wheat Streak Mosaic Virus and High Plains Virus. Picture by Dr. Gaylon Morgan, Texas Extension Small Grains Specialist. Resources Sources for the information...

Morgan, Gaylon

2005-01-26T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

DOI: 10.1002/cmdc.200800171 "Superbugs Bunny" Outsmarts Our Immune Defense  

E-Print Network [OSTI]

. aureus (MRSA)[2] has exceeded the number of HIV-associated deaths in the US.[3] Bacteria rapidly mutate population and select for re- sistant organisms.[4] Multi-resistant and hyper-virulent microbes such as MRSA have become a physician's nightmare in hospitals and in the community (e.g. CA- MRSA USA300

Nizet, Victor

42

Winter Infections: Influenza, Respiratory Syncytial Virus (RSV)  

E-Print Network [OSTI]

spread? Sick person can spread influenza by: ­ Touching ­ Sneezing ­ Coughing Picture: newbedfordguide Fever Cough Fatigue (feel "worn out") Headaches Body aches Runny nose #12;Influenza ("Flu") Treatments paralysis) #12;"MythBusters" Reported That: Sneezes can travel ~35 mph Cough/sneeze droplets can travel

Goldman, Steven A.

43

CD8? T Cell Response to Influenza Virus  

E-Print Network [OSTI]

The flu is an extremely prevalent and potentially devastating disease, especially dangerous to the very young, the elderly, and to people with compromised immune systems. Influenza has a characteristic course of infection, ...

Doty, Daniel, S.M. Massachusetts Institute of Technology

2005-01-01T23:59:59.000Z

44

From Shakespeare to Viruses  

SciTech Connect (OSTI)

Berkeley Lab scientists have created a unique new tool for analyzing and comparing long sets of data, be it the genomes of mammals or viruses, or the works of Shakespeare. The results of the Shakespeare analysis surprised scholars with their accuracy

Sung-Hou Kim

2009-02-09T23:59:59.000Z

45

Computer virus information update CIAC-2301  

SciTech Connect (OSTI)

While CIAC periodically issues bulletins about specific computer viruses, these bulletins do not cover all the computer viruses that affect desktop computers. The purpose of this document is to identify most of the known viruses for the MS-DOS and Macintosh platforms and give an overview of the effects of each virus. The authors also include information on some windows, Atari, and Amiga viruses. This document is revised periodically as new virus information becomes available. This document replaces all earlier versions of the CIAC Computer virus Information Update. The date on the front cover indicates date on which the information in this document was extracted from CIAC`s Virus database.

Orvis, W.J.

1994-01-15T23:59:59.000Z

46

LOUISIANA GUIDANCE FOR CLINICIANS ON THE NEED FOR MEDICAL EVALUATION, TESTING, AND TREATMENT OF POSSIBLE NOVEL H1N1 "SWINE"  

E-Print Network [OSTI]

viruses spread by coughing, sneezing and touching contaminated surfaces. The symptoms of the H1N1 flu virus in people are similar to the symptoms of seasonal flu and include fever, cough, sore throat, runny that the child does not want to be held Flu-like symptoms improve but then return with fever and worse cough

47

Giant viruses in the oceans : the 4th Algal Virus Workshop  

E-Print Network [OSTI]

Paramecium bursaria chlorella virus (PBCV-1), the prototype of the Phycodnaviridae, was isolated more than 20 bursaria chlorella viruses: NY-2A (infecting PBCV-1 host Chlorella species NC64A) and Chlorella Pbi virus MT325. NY-2A genome contains 368,683 bp, making it the largest chlorella virus sequenced to date

Boyer, Edmond

48

Should I Work Today? | Flu Information | DHMC Intranet http://intranet.hitchcock.org/hc/webpage.cfm?site_id=1&org_id=393&morg_id=0&gsec_id=36557&item_id=36570&printable=true[5/13/2013 2:25:34 PM  

E-Print Network [OSTI]

Lesions, rashes, and eye conditions may not apply to flu-like illness. COUGH If you have a cough and fever (>100 F or 38 C within the last 24 hours) NO If you have a new (cough, but no fever (, or aspirin) YES, but wear a mask that covers nose and mouth. If you have a prolonged cough of unknown cause

Myers, Lawrence C.

49

Selected Studies on Avian RNA Viruses  

E-Print Network [OSTI]

SELECTED STUDIES ON AVIAN RNA VIRUSES A Dissertation by ITAMAR DAVI SCHABERT VILLANUEVA Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR... OF PHILOSOPHY May 2010 Major Subject: Veterinary Microbiology Selected Studies on Avian RNA Viruses Copyright 2010 Itamar Davi Schabert Villanueva SELECTED STUDIES ON AVIAN RNA VIRUSES A...

Villanueva, Itamar D.

2010-07-14T23:59:59.000Z

50

Is It A Cold Or The Flu? Symptoms Cold Flu  

E-Print Network [OSTI]

Usual Sometimes Sore throat Common Sometimes Chest discomfort, cough Mild-moderate, hacking Common, can fever and cough with medicines you can buy at the store. 5. If you get very sick or are pregnant or have

O'Toole, Alice J.

51

NOVEL INFLUENZA A "SWINE FLU"  

E-Print Network [OSTI]

an infected person sneezes or coughs. People in close (within 3 feet) proximity can become inflected. Steps to protect yourself and others: · Washhandsoftenwithsoapandwater especially after coughs: · Feverof100.4degreesormore · Cough · Bodyaches · Runnynose · Sorethroat · Nausea · Vomiting · Diarrhea

Wiegner, Tracy N.

52

Jaloro': A New Multiple Virus Resistant Hot Yellow Jalapeno Pepper.  

E-Print Network [OSTI]

growing condi tions and lack ing heavy pressure from insects and diseases. The effects of a complex of insects, diseases, and unfavorable environment are reflected in low yields in some temporate and sub tropical regions of Texas such as the Lower... per cultivars are susceptible to to bacco etch virus (TEV), potato virus Y (PVY), pepper mottle virus (PeMV), tobacco ringspot virus (TRSV), cucumber mosaic virus (CMV), and Texas pepper gemini virus (TPGV). Some yellow types exhibit a local...

Villalon, Benigno

1992-01-01T23:59:59.000Z

53

Class II virus membrane fusion proteins  

SciTech Connect (OSTI)

Enveloped animal viruses fuse their membrane with a host cell membrane, thus delivering the virus genetic material into the cytoplasm and initiating infection. This critical membrane fusion reaction is mediated by a virus transmembrane protein known as the fusion protein, which inserts its hydrophobic fusion peptide into the cell membrane and refolds to drive the fusion reaction. This review describes recent advances in our understanding of the structure and function of the class II fusion proteins of the alphaviruses and flaviviruses. Inhibition of the fusion protein refolding reaction confirms its importance in fusion and suggests new antiviral strategies for these medically important viruses.

Kielian, Margaret [Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461 (United States)]. E-mail: kielian@aecom.yu.edu

2006-01-05T23:59:59.000Z

54

acquired immunodeficiency virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

55

affect virus replication: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

56

attenuate virus replication: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

57

archaeal viruses unique: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

58

aleutian disease virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

59

arthritis encephalitis virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

60

acquired respiratory virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

National Center for Immunization and Respiratory Diseases No More Excuses  

E-Print Network [OSTI]

to protect yourself and your family. If you miss getting your flu vaccine in the fall, make it a New Year Vaccine WrongThe flu (influenza) is a contagious disease which affects the lungs and can lead to serious protect against non-flu viruses. · Oryoumighthavebeenexposedtoflu after you got vaccinated but before

Liblit, Ben

62

Paramyxovirus fusion: Real-time measurement of parainfluenza virus 5 virus-cell fusion  

SciTech Connect (OSTI)

Although cell-cell fusion assays are useful surrogate methods for studying virus fusion, differences between cell-cell and virus-cell fusion exist. To examine paramyxovirus fusion in real time, we labeled viruses with fluorescent lipid probes and monitored virus-cell fusion by fluorimetry. Two parainfluenza virus 5 (PIV5) isolates (W3A and SER) and PIV5 containing mutations within the fusion protein (F) were studied. Fusion was specific and temperature-dependent. Compared to many low pH-dependent viruses, the kinetics of PIV5 fusion was slow, approaching completion within several minutes. As predicted from cell-cell fusion assays, virus containing an F protein with an extended cytoplasmic tail (rSV5 F551) had reduced fusion compared to wild-type virus (W3A). In contrast, virus-cell fusion for SER occurred at near wild-type levels, despite the fact that this isolate exhibits a severely reduced cell-cell fusion phenotype. These results support the notion that virus-cell and cell-cell fusion have significant differences.

Connolly, Sarah A. [Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208-3500 (United States); Lamb, Robert A. [Howard Hughes Medical Institute, Northwestern University, Evanston, IL 60208-3500 (United States) and Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208-3500 (United States)]. E-mail: ralamb@northwestern.edu

2006-11-25T23:59:59.000Z

63

Review article Molecular biology of fish viruses: a review  

E-Print Network [OSTI]

Review article Molecular biology of fish viruses: a review J Bernard, M Brémont* INRA, laboratoire aspects in the fish virus studies. Although more than 50 different fish virus have been isolated family, the fish lym- phocystis disease virus (FLDV) is the most studied. Retroviridae have been recently

Boyer, Edmond

64

E-Print Network 3.0 - arthritis-encephalitis virus surface Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

virus, Ovine lentivirus, Visna virus); and Primate... Committee (RAC). The full NIH publication is available at http:oba.od.nih.govobaracGuidanceLentiVirus... bovine...

65

Field studies of virus transport in a heterogeneous sandy aquifer  

E-Print Network [OSTI]

water. Ground water transport models can be used to predict the transport of viruses. However, if current public domain virus transport models are to be used for this purpose, they need to be verified under operating field conditions. To evaluate...

Vogel, Jason Robert

1997-01-01T23:59:59.000Z

66

Treatment of tumors with genetically engineered herpes virus  

DOE Patents [OSTI]

Disclosed are methods for treating cancer by administering an effective amount of a modified Herpes simplex virus.

Weichselbaum, Ralph R; Roizman, Bernard; Whitley, Richard J

2012-11-27T23:59:59.000Z

67

Phonon Confinement Effects in Hybrid Virus-Inorganic Nanotubes for  

E-Print Network [OSTI]

Phonon Confinement Effects in Hybrid Virus-Inorganic Nanotubes for Nanoelectronic Applications as nanotemplates, viruses can actually improve the electron transport properties in semiconductor nanotubes grown nanotubes deposited on tobacco mosaic viruses, the confined acoustic phonons are found to be redistributed

Fonoberov, Vladimir

68

Pathogenic Interactions Between Sorghum Yellow Banding Virus and Other Viruses Infecting Sorghum.  

E-Print Network [OSTI]

-infected leaves were smaller than those infected by MDMV-O and showed different s~ptoms. Leaf symptoms from the plant with all VIruses looked the same as those infected with MDMV-A alone and plant tissue was serologically positive to MDMV-A. 2 Table 3... virus sap inoculated 3 days before inoculating with SYBV - infected plant sap. 2. = Multiplication of proportionate effect for MDMV-A by SYBV. 3. = ~aize d:wvarf ~osaic virus sap inoculated 3 days before Inoculating With MDMV-O, and finally...

Theu, M.P.K.J; Toler, R.W.

1992-01-01T23:59:59.000Z

69

anemia virus p9: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

against chicken anemia virus in unvaccinated broilers and broiler breeders in Croatia CiteSeer Summary: The prevalence of antibodies against infectious chicken anemia...

70

anemia virus reveal: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

against chicken anemia virus in unvaccinated broilers and broiler breeders in Croatia CiteSeer Summary: The prevalence of antibodies against infectious chicken anemia...

71

HIV virus spread and evolution studied through computer modeling  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

HIV and evolution studied through computer modeling HIV virus spread and evolution studied through computer modeling This approach distinguishes between susceptible and infected...

72

RESPONDING TO FLU-LIKE SYMPTOMS  

E-Print Network [OSTI]

to frequently clean room surfaces. Avoid touching eyes, nose, and mouth. Cough or sneeze into your sleeve

McConnell, Terry

73

PREGNANCY AND SWINE FLU FOR THE Background  

E-Print Network [OSTI]

of appetite and cough), you should contact your GP as soon as possible and this should be done by phone. Other: · People covering their nose and mouth when coughing or sneezing, using a tissue when possible. · Disposing

Davies, Christopher

74

flu preparations Wash your hands, cover  

E-Print Network [OSTI]

and audiology. Koop currently co-leads the three-year GenomicsinLiceandSalmonprojectusing advanced genomics in Genomics and Molecular Biology. He was also part of the world-wide team of scientists who mapped the human genome. Koop's team is

Victoria, University of

75

Toward Design of a Universal Flu Vaccine  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas ConchasPassiveSubmittedStatusButler Tina ButlerToday inm"Topo II: AnLAr SToursToward

76

Battling bird flu by the numbers  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to someone6 M. Babzien, I. Ben-Zvi, P. Study ofJLeadershipBattling bird

77

Berkeley Lab Pandemic Flu Information - Home  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth (AOD)ProductssondeadjustsondeadjustAboutScienceCareers Apply for a Job External ApplicantsSearchLogin

78

Microsoft Word - 1918flu.doc  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces andMapping RichlandScatteringWater308:UFC 2300.00 Department of 1Figure 1.

79

Toward Design of a Universal Flu Vaccine  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonal Computerso w a r d C o d e

80

Toward Design of a Universal Flu Vaccine  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonal Computerso w a r d C o d

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Toward Design of a Universal Flu Vaccine  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonal Computerso w a r d C o

82

Toward Design of a Universal Flu Vaccine  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonal Computerso w a r d C oToward

83

The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus  

E-Print Network [OSTI]

bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane image reconstruction of the virus. Chlorella viruses, isolated from freshwater sources throughout and to iridoviruses (3, 4) such as Chilo iridescent virus (1). Paramecium bursaria Chlorella virus (PBCV), genus

Rossmann, Michael G.

84

E-Print Network 3.0 - animal virus replication Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Radiobiological Studies of Vaccinia Virus' Summary: of a few animal viruses (Dulbecco and Vogt, 1955 ; Frank- lin, 1958; Powell, 1959; Kaplan, 1962)) but... , owing partly to a...

85

E-Print Network 3.0 - animal viruses nsv Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Radiobiological Studies of Vaccinia Virus' Summary: of a few animal viruses (Dulbecco and Vogt, 1955 ; Frank- lin, 1958; Powell, 1959; Kaplan, 1962)) but... , owing partly to a...

86

anti-influenza virus activity: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

87

adeno-associated virus type: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

cause diseases with long incubation periods, insidious onsets, and slowly progressive courses (1). The members of the virus group include visna virus of sheep, caprine...

88

E-Print Network 3.0 - al virus huasteco Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and characterized... 499442 Pepper huasteco yellow vein virus PHYVV X70418, X70419 Potato yellow mosaic virus... ;1702 P. L. Ramos et al. Fig. 1. Diagrammatic ... Source:...

89

E-Print Network 3.0 - apple mosaic virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

translation of plant viral RNAs Summary: in capped RNAs such as those of Potato virus X (Smirnyagina et al., 1991) and Tobacco mosaic virus (Gallie... , including...

90

E-Print Network 3.0 - animal sars viruses Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Sciences 75 Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda Summary: Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in...

91

E-Print Network 3.0 - attenuated aids virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

73 Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda Summary: Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in...

92

E-Print Network 3.0 - african virus adapting Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of new Simian foamy viruses from African nonhuman Primates. Virology, 237... A. P. JACKSON AND M.A. CHARLESTON HOST RNA-VIRUS COPHYLOGENY 1 A ... Source: Charleston,...

93

Genomics of bacterial and archaeal virus isolates from extreme aquatic environments.  

E-Print Network [OSTI]

??Viruses are ubiquitous, abundant and diverse members of the biosphere. Numerous sequencing projects focusing on isolated viruses and uncultured viral communities (metaviromes) have demonstrated that (more)

Sencilo, Ana

2014-01-01T23:59:59.000Z

94

E-Print Network 3.0 - anaemia virus isav-infected Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Environmental Sciences and Ecology 90 Molecular Detection of Infectious Viruses in Water More than 140 different enteric viruses are known to infect humans causing a wide...

95

Heterosubtypic Immunity to Influenza A Virus Infections in Mallards May Explain Existence of Multiple Virus  

E-Print Network [OSTI]

and Integrate Risk Management, Campus International de Baillarguet, Montpellier, France, 3 Karolinska Institutet, Uppsala University, Uppsala, Sweden Abstract Wild birds, particularly duck species, are the main reservoir of influenza A virus (IAV) in nature. However, knowledge of IAV infection dynamics in the wild bird reservoir

Lazzaro, Brian

96

Molecular Determinants and Dynamics of Hepatitis C Virus Secretion  

E-Print Network [OSTI]

Molecular Determinants and Dynamics of Hepatitis C Virus Secretion Kelly E. Coller, Nicholas S virus (HCV) production involves the assembly of virions on or near the surface of lipid droplets. However, the cellular requirements for and a mechanistic understanding of HCV secretion are incomplete

97

Molecular biology study of satellite panicum mosaic virus capsid protein  

E-Print Network [OSTI]

Satellite panicum mosaic virus (SPMV) depends on its helper Panicum mosaic virus (PMV) for replication and movement in host plants. The positive-sense single-stranded genomic RNA of SPMV encodes a 17-kDa capsid protein (CP) to form 16-nm virions...

Qi, Dong

2009-05-15T23:59:59.000Z

98

The Pathogenesis of Cache Valley Virus in the Ovine Fetus  

E-Print Network [OSTI]

demonstrated that after subcutaneous inoculation, the virus replicates in smooth muscle cells of blood vessels and later enter the reticulo-endothelial system with a short 1-3 day viremia. After primary viremia, the viruses cross the blood-brain barrier...

Rodrigues, Aline

2012-02-14T23:59:59.000Z

99

Intracellular transactivation of HIV can account for the decelerating decay of virus load during drug therapy  

E-Print Network [OSTI]

between an activated and latent state results in the typical decelerating decay of virus load following

Utrecht, Universiteit

100

Genetic diversity in chlorella viruses flanking kcv, a gene that encodes a potassium ion channel protein  

E-Print Network [OSTI]

Genetic diversity in chlorella viruses flanking kcv, a gene that encodes a potassium ion channel Received 23 March 2004; accepted 27 May 2004 Abstract The chlorella virus PBCV-1 encodes a 94-amino acid additional chlorella viruses and transcription in two of these viruses, as well as the effect of the three

Graves, Michael V.

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Molecular and Genetic Evidence for a Virus-Encoded Glycosyltransferase Involved in Protein Glycosylation1  

E-Print Network [OSTI]

capsid protein, Vp54, of chlorella virus PBCV-1 is a glycoprotein that contains either one glycan of 30-encoded glycosyltransferase involved in protein glycosylation. © 2001 Academic Press Key Words: chlorella virus; PBCV-1; Phycodnaviridae; virus glycoprotein; glycosyltransferase. INTRODUCTION Paramecium bursaria chlorella virus-1 (PBCV

Graves, Michael V.

102

Virus Research 140 (2009) 138146 Contents lists available at ScienceDirect  

E-Print Network [OSTI]

homepage: www.elsevier.com/locate/virusres The A312L 5 -UTR of Chlorella virus PBCV-1 is a translational Phycodnaviridae Chlorella viruses A312L gene Arabidopsis thaliana Agrobacterium-mediated transformation a b s t r a c t PBCV-1 (Paramecium bursaria Chlorella virus) is a large double stranded DNA virus

Graves, Michael V.

103

An icosahedral algal virus has a complex unique vertex decorated by a spike  

E-Print Network [OSTI]

bursaria Chlorella virus-1 is an icosahedrally shaped, 1,900-?-diameter virus that infects unicellular cryoelectron microscopy reconstruction Paramecium bursaria Chlorella virus-1 cell entry specialized vertex protein Paramecium bursaria Chlorella virus-1 (PBCV-1) belongs to the genus Chlorovirus

Baker, Timothy S.

104

Nipah virus entry can occur by macropinocytosis  

SciTech Connect (OSTI)

Nipah virus (NiV) is a zoonotic biosafety level 4 paramyxovirus that emerged recently in Asia with high mortality in man. NiV is a member, with Hendra virus (HeV), of the Henipavirus genus in the Paramyxoviridae family. Although NiV entry, like that of other paramyxoviruses, is believed to occur via pH-independent fusion with the host cell's plasma membrane we present evidence that entry can occur by an endocytic pathway. The NiV receptor ephrinB2 has receptor kinase activity and we find that ephrinB2's cytoplasmic domain is required for entry but is dispensable for post-entry viral spread. The mutation of a single tyrosine residue (Y304F) in ephrinB2's cytoplasmic tail abrogates NiV entry. Moreover, our results show that NiV entry is inhibited by constructions and drugs specific for the endocytic pathway of macropinocytosis. Our findings could potentially permit the rapid development of novel low-cost antiviral treatments not only for NiV but also HeV.

Pernet, Olivier; Pohl, Christine; Ainouze, Michelle; Kweder, Hasan [Molecular Basis of Paramyxovirus Entry, INSERM U758 Virologie Humaine IFR 128 BioSciences Gerland-Lyon Sud, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07 (France); Buckland, Robin, E-mail: robin.buckland@inserm.f [Molecular Basis of Paramyxovirus Entry, INSERM U758 Virologie Humaine IFR 128 BioSciences Gerland-Lyon Sud, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07 (France)

2009-12-20T23:59:59.000Z

105

Approved by the UHS Patient Education Committee Page 1 of 2 Revised 05/09/2012  

E-Print Network [OSTI]

for the flu. What are flu symptoms? Fever (usually 100 degrees or greater) and cough and/or sore throat alcohol-based hand sanitizers after coughing, sneezing and wiping your nose to reduce the spread of the virus. Cover all coughs and sneezes with a disposable tissue and discard immediately into a trash can

106

UHS Health Promotion Office, 110 Anderson Tower, 273-5775 The Monthly InSTALLment November 2007  

E-Print Network [OSTI]

coughing or sneezing, or by touching something with flu viruses on it and then touching your mouth or nose suddenly. Flu symptoms include fever (usually high), headache, extreme tiredness, dry cough, sore throat who are sick. If you are sick, stay home from work and school to help protect others. Cover your cough

Mahon, Bradford Z.

107

Virus Assemblies as Templates for Nanocircuits  

SciTech Connect (OSTI)

The goals of this project were directed at the identification and characterization of bio-mineralization processes and patterning methods for the development of nano scale materials and structures with novel energy and conductive traits. This project utilized a simple plant virus as a model template to investigate methods to attach and coat metals and other inorganic compounds onto biologically based nanotemplates. Accomplishments include: the development of robust biological nanotemplates with enhanced inorganic coating activities; novel coating strategies that allow for the deposition of a continuous inorganic layer onto a bio-nanotemplate even in the absence of a reducing agent; three-dimensional patterning methods for the assemble of nano-featured high aspect ratio surfaces and the demonstrated use of these surfaces in enhancing battery and energy storage applications. Combined results from this project have significantly advanced our understanding and ability to utilize the unique self-assembly properties of biologically based molecules to produce novel materials at the nanoscale level.

James N Culver; Michael T Harris

2011-08-31T23:59:59.000Z

108

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer Print Immortality is not a good thing for cells, and in fact, cells will destroy themselves in a process called...

109

Host/virus interactions in the marine cyanobacterium prochlorococcus  

E-Print Network [OSTI]

Bacterial viruses shape the diversity, metabolic function, and community dynamics of their microbial hosts. As microbes drive many major biogeochemical cycles, viral infection is therefore a phenomenon of global significance. ...

Frois-Moniz, Katya

2014-01-01T23:59:59.000Z

110

Auxiliary metabolic genes in viruses infecting marine cyanobacteria  

E-Print Network [OSTI]

Marine viruses shape the diversity and biogeochemical role of their microbial hosts. Cyanophages that infect the cyanobacteria Prochlorococcus and Synechococcus often carry metabolic genes not found in other bacteriophages. ...

Thompson, Luke Richard

2010-01-01T23:59:59.000Z

111

archaeal virus acidianus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Genomics of bacterial and archaeal virus isolates from extreme aquatic environments. Open...

112

airborne viruses feasibility: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Physics Websites Summary: Modelling the spread of foot-and-mouth disease virus F Moutou B Durand CNEVA, Laboratoire Central-and-mouth disease is an economically important viral...

113

animal virus diseases: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Physics Websites Summary: Modelling the spread of foot-and-mouth disease virus F Moutou B Durand CNEVA, Laboratoire Central-and-mouth disease is an economically important viral...

114

West Nile virus transmission in resident birds, Dominican Republic  

E-Print Network [OSTI]

We report West Nile virus (WNV) activity in the Dominican Republic for the first time. Specific anti-WNV antibodies were detected in 5 (15%) of 33 resident birds sampled at one location in November 2002. One seropositive bird ...

Komar, Oliver; Robbins, Mark B.; Klenk, Kaci; Blitvich, Bradley J.; Marlenee, Nicole L.; Burkhalter, Kristen L.; Gubler, Duane J.; Gonzá lvez, Guillermo; Peñ a, Carlos J.; Peterson, A. Townsend; Komar, Nicholas

2003-10-01T23:59:59.000Z

115

Nanomachines: How Viruses Work, and How We Can Stop Them  

ScienceCinema (OSTI)

Nature's Nasty Nanomachines: How Viruses Work, and How We Can Stop Them. Carolyn Bertozzi, director of Berkeley Lab's Molecular Foundry, discusses this topic at a Feb. 21, 2009 Nano*High talk.

Carolyn Bertozzi

2010-01-08T23:59:59.000Z

116

african horse sickness virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

vectors may carry the virus into AHS-free regions. Some authors speculate that global warming could increase the risk for spread of arthropod-borne diseases such as African horse...

117

Icosadeltahedral geometry of fullerenes, viruses and geodesic domes  

E-Print Network [OSTI]

I discuss the symmetry of fullerenes, viruses and geodesic domes within a unified framework of icosadeltahedral representation of these objects. The icosadeltahedral symmetry is explained in details by examination of all of these structures. Using Euler's theorem on polyhedra, it is shown how to calculate the number of vertices, edges, and faces in domes, and number of atoms, bonds and pentagonal and hexagonal rings in fullerenes. Caspar-Klug classification of viruses is elaborated as a specific case of icosadeltahedral geometry.

Antonio Siber

2007-11-22T23:59:59.000Z

118

High molecular weight polysaccharide that binds and inhibits virus  

DOE Patents [OSTI]

This invention provides a high molecular weight polysaccharide capable of binding to and inhibiting virus and related pharmaceutical formulations and methods on inhibiting viral infectivity and/or pathogenicity, as well as immunogenic compositions. The invention further methods of inhibiting the growth of cancer cells and of ameliorating a symptom of aging. Additionally, the invention provides methods of detecting and/or quantifying and/or isolating viruses.

Konowalchuk, Thomas W

2014-01-14T23:59:59.000Z

119

Asymmetric packaging of polymerases within vesicular stomatitis virus  

SciTech Connect (OSTI)

Highlights: The VSV polymerases (L proteins) are localized to the blunt end of the virus. The VSV phosphoproteins (P proteins) are localized to the blunt end of the virus. Each VSV virion packages a variable number of P and L proteins. -- Abstract: Vesicular stomatitis virus (VSV) is a prototypic negative sense single-stranded RNA virus. The bullet-shape appearance of the virion results from tightly wound helical turns of the nucleoprotein encapsidated RNA template (N-RNA) around a central cavity. Transcription and replication require polymerase complexes, which include a catalytic subunit L and a template-binding subunit P. L and P are inferred to be in the cavity, however lacking direct observation, their exact position has remained unclear. Using super-resolution fluorescence imaging and atomic force microscopy (AFM) on single VSV virions, we show that L and P are packaged asymmetrically towards the blunt end of the virus. The number of L and P proteins varies between individual virions and they occupy 57 12 nm of the 150 nm central cavity of the virus. Our finding positions the polymerases at the opposite end of the genome with respect to the only transcriptional promoter.

Hodges, Jeffery; Tang, Xiaolin; Landesman, Michael B. [Dept. of Physics and Astronomy, University of Utah (United States) [Dept. of Physics and Astronomy, University of Utah (United States); Center for Cell and Genome Science, University of Utah (United States); Ruedas, John B. [Dept. of Biology, San Diego State University (United States)] [Dept. of Biology, San Diego State University (United States); Ghimire, Anil [Dept. of Physics and Astronomy, University of Utah (United States)] [Dept. of Physics and Astronomy, University of Utah (United States); Gudheti, Manasa V. [Vutara, Inc., Salt Lake City, UT (United States) [Vutara, Inc., Salt Lake City, UT (United States); Dept. of Biology, University of Utah (United States); Perrault, Jacques [Dept. of Biology, San Diego State University (United States)] [Dept. of Biology, San Diego State University (United States); Jorgensen, Erik M. [Howard Hughes Medical Institute (United States) [Howard Hughes Medical Institute (United States); Dept. of Biology, University of Utah (United States); Gerton, Jordan M. [Dept. of Physics and Astronomy, University of Utah (United States) [Dept. of Physics and Astronomy, University of Utah (United States); Dept. of Bioengineering, University of Utah (United States); Saffarian, Saveez, E-mail: saffarian@physics.utah.edu [Dept. of Physics and Astronomy, University of Utah (United States) [Dept. of Physics and Astronomy, University of Utah (United States); Center for Cell and Genome Science, University of Utah (United States); Dept. of Biology, University of Utah (United States)

2013-10-18T23:59:59.000Z

120

Is it a Cold or the Flu? -Know the Difference Signs & Symptoms Cold Flu  

E-Print Network [OSTI]

Rare Usual; high (100.40 F to 1020 F) is typical; lasts 3-4 days Cough Hacking: mild Dry; can become discomfort Mild to moderate; hacking cough Common Treatment Antihistamines Decongestants Advil® (ibuprofen. Avoid close contact with anyone with a cold. Vaccination. Wash your hands. Cover your cough. Stay

Mahon, Bradford Z.

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Avoid the Flu Healthy habits and simple actions will help reduce your risk of flu  

E-Print Network [OSTI]

illnesses. Wash your hands. Use soap and water often, especially after coughing or sneezing. Carry alcohol getting sick. Cover your cough. Use a tissue when coughing or sneezing. It can prevent the spread of germs. If you don't have a tissue, cough or sneeze into your arm above your hand. Don't contaminate. Try

Ferrara, Katherine W.

122

Environmental assessment of curly top virus control in California. [Curly Top Virus  

SciTech Connect (OSTI)

Curly top is a viral disease of sugarbeets, tomatoes, melons, peppers, beans, cucumbers, squash, pumpkins, spinach, vineseed and other commercially important crops, including ornamentals. Curly top not only infects commercial crops, but at times devastates backyard vegetable and flower gardens. The only known vector of curly top is Circulifer tenellus, commonly known as the beet leafhopper. Control of the beet leafhopper may take place at various locations throughout the San Joaquin Valley, Salinas Valley, Cuyama Valley, Antelope Valley of northern Los Angeles County, Riverside County and the Imperial Valley (See Maps in Appendix E''), including portions of Merced, Fresno, Kings. Kern, San Luis Obispo, Monterey, San Benito, Santa Barbara, Los Angeles, Riverside and Imperial Counties. The size of the control program is totally dependent on the location, size, nd distribution of the beet leafhopper population. In a year with a low population, only 80,000 acres in western Fresno, Kings, and Kern Counties may require treatment. In a drought year, the treatment required may increase to more than 200,000 acres and include some inter-coastal valleys of Monterey, San Luis Obispo and Santa Barbara Counties. Not all areas require treatment on an annual basis and at no time is the entire area subject to blanket treatment. The Curly Top Virus Control Program (CTVCP) was instrumental in supporting research which developed an antisera allowing the use of Enzyme-Linked Immunosorbent Assay to determine the presence, on a percentage basis, of curly top virus. Thus, the amount of virus found in a given area lends weight to treatment priorities.

Not Available

1991-03-15T23:59:59.000Z

123

Visualization of the African swine fever virus infection in living cells by incorporation into the virus particle of green fluorescent protein-p54 membrane protein chimera  

SciTech Connect (OSTI)

Many stages of African swine fever virus infection have not yet been studied in detail. To track the behavior of African swine fever virus (ASFV) in the infected cells in real time, we produced an infectious recombinant ASFV (B54GFP-2) that expresses and incorporates into the virus particle a chimera of the p54 envelope protein fused to the enhanced green fluorescent protein (EGFP). The incorporation of the fusion protein into the virus particle was confirmed immunologically and it was determined that p54-EGFP was fully functional by confirmation that the recombinant virus made normal-sized plaques and presented similar growth curves to the wild-type virus. The tagged virus was visualized as individual fluorescent particles during the first stages of infection and allowed to visualize the infection progression in living cells through the viral life cycle by confocal microscopy. In this work, diverse potential applications of B54GFP-2 to study different aspects of ASFV infection are shown. By using this recombinant virus it was possible to determine the trajectory and speed of intracellular virus movement. Additionally, we have been able to visualize for first time the ASFV factory formation dynamics and the cytophatic effect of the virus in live infected cells. Finally, we have analyzed virus progression along the infection cycle and infected cell death as time-lapse animations.

Hernaez, Bruno [Departamento de Biotecnologia, INIA, Carretera de la Coruna Km 7, 28040 Madrid (Spain)]. E-mail: hernaez@inia.es; Escribano, Jose M. [Departamento de Biotecnologia, INIA, Carretera de la Coruna Km 7, 28040 Madrid (Spain)]. E-mail: escriban@inia.es; Alonso, Covadonga [Departamento de Biotecnologia, INIA, Carretera de la Coruna Km 7, 28040 Madrid (Spain)]. E-mail: calonso@inia.es

2006-06-20T23:59:59.000Z

124

The role of feline syncytium forming virus in feline immunodeficiency virus-mediated acquired immunodeficiency  

E-Print Network [OSTI]

) and feline syncytium-forming virus (FeSFV) alone and in combination were studied to help define the role of FeSFV as a potentiating cofactor in FIV-mediated acquired immunodeficiency syndrome (AIDS). Healthy specific pathogen free (SPF) cats age 9 months... were divided into 2 groups for the first phase of the experiment: 13 cats were experimentally infected with FeSFV and 12 cats served as uninfected controls. Four months following FeSFV inoculation, 7 FeSFV positive and 7 FeSFV negative cats were...

Zenger, Elizabeth

1991-01-01T23:59:59.000Z

125

acute neurological symptoms: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the common cold or flu, the mumps virus spreads in the air from an infected persons cough or sneeze. A child also can get infected with mumps by coming in contact with an...

126

DeclanButler,Paris The mass culling of poultry to contain  

E-Print Network [OSTI]

DeclanButler,Paris The mass culling of poultry to contain outbreaks of avian flu is no longer. This means that no matter how often affected poultry flocks are culled, the virus is likely to reappear

Cai, Long

127

anti-virus papiloma humano: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

writers and anti-virus defenders. The simple computer virus has evolved into more complex stealth Bishop, Matt 70 Guide to Accessing the Osher Online Video Library 1) Visit the...

128

active epstein-barr virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

M2 Proton Channel from Influenza A Virus and a Structural Model for Channel Activation Materials Science Websites Summary: The M2 Proton Channel from Influenza A Virus and a...

129

NMR STUDIES OF THE GLYCOPROTEIN CYTOPLASMIC TAILS OF HANTAVIRUS AND CRIMEAN CONGO HEMORRHAGIC FEVER VIRUS  

E-Print Network [OSTI]

The Bunyaviridae family of viruses is a diverse grouping of approximately 350 members that is present throughout the world. Viruses range from the innocuous to the severely pathogenic and impact not only public health, but ...

Estrada, David Fernando

2011-04-25T23:59:59.000Z

130

Datasets: Phylogenetic assessment of filoviruses: how many lineages of Marburg virus?  

E-Print Network [OSTI]

Filoviruses have to date been considered as consisting of one diverse genus (Ebola viruses) and one undifferentiated genus (Marburg virus). We reconsider this idea by means of detailed phylogenetic analyses of sequence ...

Peterson, A. Townsend; Holder, Mark T.

2012-06-08T23:59:59.000Z

131

The Capsid Proteins of a Large, Icosahedral dsDNA Virus  

E-Print Network [OSTI]

Paramecium bursaria chlorella virus 1 Vp54 MCP. This model was fitted into the cryoEM density for eachEM, cryoelectron microscopy; MCP, major capsid protein; PBCV-1, Paramecium bursaria chlorella virus type 1; PpV01

Baker, Timothy S.

132

Platforms for exploring host-pathogen interactions in hepatitis C virus infection  

E-Print Network [OSTI]

Afflicting almost 200 million worldwide, hepatitis C virus (HCV) mounts a chronic infection of liver hepatocytes that causes substantial morbidity and mortality. An understanding of host-virus interactions will drive the ...

Trehan, Kartik

2012-01-01T23:59:59.000Z

133

E-Print Network 3.0 - alfalfa mosaic virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

virus Search Powered by Explorit Topic List Advanced Search Sample search results for: alfalfa mosaic virus Page: << < 1 2 3 4 5 > >> 1 Plant Disease February 2006 203 Incidence,...

134

E-Print Network 3.0 - abutilon mosaic virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178... of Potato yellow mosaic virus from the Caribbean Brief Report C. Urbino1, J. E. Polston2, C. P....

135

E-Print Network 3.0 - adenovirus influenza virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

17 VII. Related Products 18 List of Figures Figure 1. Principle of Adeno-X Virus... as effective (Table I). A chromatographic method The Adeno-X Virus Purification Kit lets...

136

E-Print Network 3.0 - aislamiento del virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

del virus Page: << < 1 2 3 4 5 > >> 1 An Avian Influenza Virus from Waterfowl in South America Contains Genes from North American Avian and Equine Lineages Summary: de...

137

Characterization of a -1,3-Glucanase Encoded by Chlorella Virus PBCV-11 Liangwu Sun,*,2  

E-Print Network [OSTI]

Characterization of a -1,3-Glucanase Encoded by Chlorella Virus PBCV-11 Liangwu Sun,*,2 James R of the 330-kb chlorella virus PBCV-1 genome revealed an open-reading frame, A94L, that encodes a protein.i. postinfection, respectively, indicating that a94l is an early gene. Twenty-seven of 42 chlorella viruses

Graves, Michael V.

138

Final report [FASEB Summer Research Conference ''Virus Assembly''--agenda and attendee list  

SciTech Connect (OSTI)

The conference brought together researchers working on virus structure and virus assembly in diverse systems. Information was integrated from many viral systems, including plant bacterial and eukaryotic viruses, and many techniques such as biophysical approaches of x-ray diffraction, electron microscopy and spectroscopy, along with molecular biological and molecular genetic analysis.

Feiss, Michael

2001-01-31T23:59:59.000Z

139

Accepted Manuscript High occurrence of Hepatitis E virus in samples from wastewater treatment plants in  

E-Print Network [OSTI]

Accepted Manuscript High occurrence of Hepatitis E virus in samples from wastewater treatment-Bianchi, D., Oppliger, A., High occurrence of Hepatitis E virus in samples from wastewater treatment plants MANUSCRIPT Highlights Hepatitis E virus (HEV) was searched in raw and treated wastewater in Switzerland

Alvarez, Nadir

140

Alfalfa mosaic virus replicase proteins, P1 and P2, localize to the tonoplast in the presence of virus RNA  

SciTech Connect (OSTI)

To identify the virus components important for assembly of the Alfalfa mosaic virus replicase complex, we used live cell imaging of Arabidopsis thaliana protoplasts that expressed various virus cDNAs encoding native and GFP-fusion proteins of P1 and P2 replicase proteins and full-length virus RNAs. Expression of P1-GFP alone resulted in fluorescent vesicle-like bodies in the cytoplasm that colocalized with FM4-64, an endocytic marker, and RFP-AtVSR2, RabF2a/Rha1-mCherry, and RabF2b/Ara7-mCherry, all of which localize to multivesicular bodies (MVBs), which are also called prevacuolar compartments, that mediate traffic to the lytic vacuole. GFP-P2 was driven from the cytosol to MVBs when expressed with P1 indicating that P1 recruited GFP-P2. P1-GFP localized on the tonoplast, which surrounds the vacuole, in the presence of infectious virus RNA, replication competent RNA2, or P2 and replication competent RNA1 or RNA3. This suggests that a functional replication complex containing P1, P2, and a full-length AMV RNA assembles on MVBs to traffic to the tonoplast.

Ibrahim, Amr [Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907 (United States) [Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907 (United States); Present address: Genomics Facility, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza 12619 (Egypt); Hutchens, Heather M. [Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907 (United States)] [Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907 (United States); Howard Berg, R. [Integrated Microscopy Facility, Donald Danforth Plant Science Center, Saint Louis, MO 63132 (United States)] [Integrated Microscopy Facility, Donald Danforth Plant Science Center, Saint Louis, MO 63132 (United States); Sue Loesch-Fries, L., E-mail: loeschfr@purdue.edu [Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907 (United States)

2012-11-25T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Reverse Genetics System for Mouse Hepatitis Virus Strain 1  

E-Print Network [OSTI]

the virus has entered the target cell, it begins to translate viral replication proteins and then to copy the genome in order to access the structural genes needed to make progeny virus. The entire replication cycle takes place in the cytoplasm of the host... to the 3? UTR of MHV, it does not provide access to genes upstream of the S gene, leaving only 1/3 of the genome available for analysis (50). The other two thirds of the genome consists of ORF1a and ORF1b, which contain the replicase genes. These genes...

Carter, Kristen

2011-04-19T23:59:59.000Z

142

Viruses in the plankton of freshwater and saline Antarctic lakes  

E-Print Network [OSTI]

¯agellates and other protists, and thereby short-circuit the microbial loop, reducing the transfer of carbon organic carbon concentration (r 0.845, P , it is likely that viruses play a pivotal role in carbon cycling in these extreme ecosystems. Keywords

Sommaruga, Ruben

143

2005 Nature Publishing Group Photosynthesis genes in marine viruses yield  

E-Print Network [OSTI]

2005 Nature Publishing Group Photosynthesis genes in marine viruses yield proteins during host6 probably influences the genetic and functional diversity of both. For example, photosynthesis genes period. We also show that the expression of host photosynthesis genes declines over the course

Church, George M.

144

Fate of human viruses in groundwater recharge systems  

SciTech Connect (OSTI)

The overall objective of this research program was to determine the ability of a well-managed tertiary effluent-recharge system to return virologically acceptable water to the groundwater aquifer. The study assessed the quality of waters renovated by indigenous recharge operations and investigated a number of virus-soil interrelationships. The elucidation of the interactions led to the establishment of basin operating criteria for optimizing virus removal. Raw influents, chlorinated tertiary effluents, and renovated wastewater from the aquifer directly beneath a uniquely designed recharge test basin were assayed on a weekly basis for the presence of human enteroviruses and coliform bacteria. High concentrations of viruses were routinely isolated from influents but were isolated only on four occasions from tertiary-treated sewage effluents. In spite of the high quality effluent being recharged, viruses were isolated from the groundwater observation well, indicating their ability to penetrate the unsaturated zone. Results of poliovirus seeding experiments carried out in the test basin clearly indicated the need to operate recharge basins at low (e.g. 1 cm/h) infiltration rates in areas having soil types similar to those found at the study site. The method selected for reducing the test basin infiltration rate involved clogging the basin surface with settled organic material from highly turbid effluent. Alternative methods for slowing infiltration rates are discussed in the text.

Vaughn, J.M.; Landry, E.F.

1980-03-01T23:59:59.000Z

145

Vorticity banding in rodlike virus suspensions Kyongok Kang,1  

E-Print Network [OSTI]

Vorticity banding in rodlike virus suspensions Kyongok Kang,1 M. P. Lettinga,1 Z. Dogic,2 and Jan K. G. Dhont1 1 Institute für Festkörper Forschung (IFF), Weiche Materie, Forschungszentrum Jülich, D

Fraden, Seth

146

Immunogenicity and protective efficacy of Semliki forest virus replicon-based DNA vaccines encoding goatpox virus structural proteins  

SciTech Connect (OSTI)

Goatpox, caused by goatpox virus (GTPV), is an acute feverish and contagious disease in goats often associated with high morbidity and high mortality. To resolve potential safety risks and vaccination side effects of existing live attenuated goatpox vaccine (AV41), two Semliki forest virus (SFV) replicon-based bicistronic expression DNA vaccines (pCSm-AAL and pCSm-BAA) which encode GTPV structural proteins corresponding to the Vaccinia virus proteins A27, L1, A33, and B5, respectively, were constructed. Then, theirs ability to induce humoral and cellular response in mice and goats, and protect goats against virulent virus challenge were evaluated. The results showed that, vaccination with pCSm-AAL and pCSm-BAA in combination could elicit strong humoral and cellular responses in mice and goats, provide partial protection against viral challenge in goats, and reduce disease symptoms. Additionally, priming vaccination with the above-mentioned DNA vaccines could significantly reduce the goats' side reactions from boosting vaccinations with current live vaccine (AV41), which include skin lesions at the inoculation site and fevers. Data obtained in this study could not only facilitate improvement of the current goatpox vaccination strategy, but also provide valuable guidance to suitable candidates for evaluation and development of orthopoxvirus vaccines.

Zheng Min [Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062 (China); Guangxi Center for Animal Disease Control and Prevention, Nanning 530001 (China); College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062 (China); Jin Ningyi, E-mail: jinningyi2000@yahoo.com.c [Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062 (China); Liu Qi, E-mail: gx_liuqi@yahoo.com.c [Guangxi Center for Animal Disease Control and Prevention, Nanning 530001 (China); Huo Xiaowei [Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062 (China); Li Yang [Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062 (China); College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062 (China); Hu Bo; Ma Haili; Zhu Zhanbo; Cong Yanzhao; Li Xiao; Jin Minglan; Zhu Guangze [Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062 (China)

2009-08-15T23:59:59.000Z

147

A chimeric measles virus with a lentiviral envelope replicates exclusively in CD4+/CCR5+ cells  

SciTech Connect (OSTI)

We generated a replicating chimeric measles virus in which the hemagglutinin and fusion surface glycoproteins were replaced with the gp160 envelope glycoprotein of simian immunodeficiency virus (SIVmac239). Based on a previously cloned live-attenuated Schwarz vaccine strain of measles virus (MV), this chimera was rescued at high titers using reverse genetics in CD4+ target cells. Cytopathic effect consisted in the presence of large cell aggregates evolving to form syncytia, as observed during SIV infection. The morphology of the chimeric virus was identical to that of the parent MV particles. The presence of SIV gp160 as the only envelope protein on chimeric particles surface altered the cell tropism of the new virus from CD46+ to CD4+ cells. Used as an HIV candidate vaccine, this MV/SIVenv chimeric virus would mimic transient HIV-like infection, benefiting both from HIV-like tropism and the capacity of MV to replicate in dendritic cells, macrophages and lymphocytes.

Mourez, Thomas [Unite de Genomique Virale et Vaccination, CNRS URA 3015, Institut Pasteur, F-75015 Paris (France); APHP, GH Saint-Louis-Lariboisiere, Laboratoire de Bacteriologie-Virologie, F-75010 Paris (France); Universite Paris 7 Denis Diderot, F-75010 Paris (France); Mesel-Lemoine, Mariana; Combredet, Chantal; Najburg, Valerie [Unite de Genomique Virale et Vaccination, CNRS URA 3015, Institut Pasteur, F-75015 Paris (France); Cayet, Nadege [Institut Pasteur, Plateforme de Microscopie Ultrastructurale, F-75015 Paris (France); Tangy, Frederic, E-mail: ftangy@pasteur.fr [Unite de Genomique Virale et Vaccination, CNRS URA 3015, Institut Pasteur, F-75015 Paris (France)

2011-10-25T23:59:59.000Z

148

The stochastic entry of enveloped viruses: Fusion vs. endocytosis  

E-Print Network [OSTI]

Viral infection requires the binding of receptors on the target cell membrane to glycoproteins, or ``spikes,'' on the viral membrane. The initial entry is usually classified as fusogenic or endocytotic. However, binding of viral spikes to cell surface receptors not only initiates the viral adhesion and the wrapping process necessary for internalization, but can simultaneously initiate direct fusion with the cell membrane. Both fusion and internalization have been observed to be viable pathways for many viruses. We develop a stochastic model for viral entry that incorporates a competition between receptor mediated fusion and endocytosis. The relative probabilities of fusion and endocytosis of a virus particle initially nonspecifically adsorbed on the host cell membrane are computed as functions of receptor concentration, binding strength, and number of spikes. We find different parameter regimes where the entry pathway probabilities can be analytically expressed. Experimental tests of our mechanistic hypotheses are proposed and discussed.

Tom Chou

2007-04-13T23:59:59.000Z

149

Virus-Enabled Silicon Anode for Lithium-Ion Batteries  

SciTech Connect (OSTI)

A novel three-dimensional Tobacco mosaic virus assembled silicon anode is reported. This electrode combines genetically modified virus templates for the production of high aspect ratio nanofeatured surfaces with electroless deposition to produce an integrated nickel current collector followed by physical vapor deposition of a silicon layer to form a high capacity silicon anode. This composite silicon anode produced high capacities (3300 mAh/g), excellent charge?discharge cycling stability (0.20% loss per cycle at 1C), and consistent rate capabilities (46.4% at 4C) between 0 and 1.5 V. The biological templated nanocomposite electrode architecture displays a nearly 10-fold increase in capacity over currently available graphite anodes with remarkable cycling stability.

Chen, X L; Gerasopoulos, K; Guo, J C; Brown, A; Wang, Chunsheng; Ghodssi, Reza; Culver, J N

2010-01-01T23:59:59.000Z

150

Development of simulation tools for virus shell assembly. Final report  

SciTech Connect (OSTI)

Prof. Berger's major areas of research have been in applying computational and mathematical techniques to problems in biology, and more specifically to problems in protein folding and genomics. Significant progress has been made in the following areas relating to virus shell assembly: development has been progressing on a second-generation self-assembly simulator which provides a more versatile and physically realistic model of assembly; simulations are being developed and applied to a variety of problems in virus assembly; and collaborative efforts have continued with experimental biologists to verify and inspire the local rules theory and the simulator. The group has also worked on applications of the techniques developed here to other self-assembling structures in the material and biological sciences. Some of this work has been conducted in conjunction with Dr. Sorin Istrail when he was at Sandia National Labs.

Berger, Bonnie

2001-01-05T23:59:59.000Z

151

Ecological and Molecular Characterization of Avian Influenza Viruses Obtained from Waterfowl on the Texas Coast  

E-Print Network [OSTI]

combinations isolated. Similar to most surveillance studies, we found no significant difference in AIV infection based on host sex, but did find that juveniles were more likely to be positive for AIV than adults. We also documented that dabbling ducks were................................................... 7 1.2 Ecology of type A influenza viruses ................................ 7 1.2.1 Wild aquatic birds and avian influenza viruses............ 9 1.2.2 Surveillance for avian influenza virus in wild aquatic...

Ferro, Pamela Joyce

2011-10-21T23:59:59.000Z

152

Adaptive evolution of simian immunodeficiency viruses isolated from two conventional progressor macaques with neuroaids  

SciTech Connect (OSTI)

Simian immunodeficiency virus infection of macaques may result in neuroAIDS, a feature more commonly observed in macaques with rapid progressive disease than in those with conventional disease. This is the first report of two conventional progressors (H631 and H636) with encephalitis in rhesus macaques inoculated with a derivative of SIVsmES43-3. Phylogenetic analyses of viruses isolated from the cerebral spinal fluid (CSF) and plasma from both animals demonstrated tissue compartmentalization. Additionally, virus from the central nervous system (CNS) was able to infect primary macaque monocyte-derived macrophages more efficiently than virus from plasma. Conversely, virus isolated from plasma was able to replicate better in peripheral blood mononuclear cells than virus from CNS. We speculate that these viruses were under different selective pressures in their separate compartments. Furthermore, these viruses appear to have undergone adaptive evolution to preferentially replicate in their respective cell targets. Analysis of the number of potential N-linked glycosylation sites (PNGS) in gp160 showed that there was a statistically significant loss of PNGS in viruses isolated from CNS in both macaques compared to SIVsmE543-3. Moreover, virus isolated from the brain in H631, had statistically significant loss of PNGS compared to virus isolated from CSF and plasma of the same animal. It is possible that the brain isolate may have adapted to decrease the number of PNGS given that humoral immune selection pressure is less likely to be encountered in the brain. These viruses provide a relevant model to study the adaptations required for SIV to induce encephalitis.

Foley, Brian T [Los Alamos National Laboratory; Korber, Bette T [Los Alamos National Laboratory

2008-01-01T23:59:59.000Z

153

tchen@engr.smu.edu Trends in Viruses  

E-Print Network [OSTI]

virus/worm attacks 82% 83% 90% 85% 94% *2003 CSI/FBI Computer Crime and Security Survey 85% 82% #12;TC) $75K $55K $45K $180K $243K *2003 CSI/FBI Computer Crime and Security Survey $283K $200K #12;TC/IEEE/10Notes, MITRE CVEs,... SANS/FBI's Top 10 Microsoft Windows vulnerabilities (May 2003): My Computer... Worms

Chen, Thomas M.

154

E-Print Network 3.0 - anticuerpos contra virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of Zoology, University of Cambridge Collection: Environmental Sciences and Ecology 53 NTU Supported Anti-Virus products for NTUanywhere AVG 9 and 10 free edition Summary: NTU...

155

E-Print Network 3.0 - anti-herpes simplex virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

with herpes simplex virus (HSV).Using congenic strains of mice, we found that the lgh... immune factors influence the clinical expres- sion of herpes ... Source: Knipe,...

156

E-Print Network 3.0 - aids viruses originate Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Immunology, University of Illinois at Chicago Collection: Biology and Medicine 33 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

157

E-Print Network 3.0 - african horsesickness virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Biology, Harvard University Collection: Environmental Sciences and Ecology 32 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

158

E-Print Network 3.0 - archaeal virus atv Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

... Source: Ecole Polytechnique, Centre de mathmatiques Collection: Mathematics 49 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

159

E-Print Network 3.0 - asocia al virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

89 Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea Summary: both fresh and marine waters...

160

E-Print Network 3.0 - aino virus infection Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Information Sciences 83 Hyaluronan Synthesis in Virus PBCV-1-Infected Chlorella-like Green Algae1 Michael V. Graves,* Dwight E. Burbank,* Robyn Roth, John Heuser,...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

E-Print Network 3.0 - archaeal virus isolated Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Universit Blaise Pascal Clermont-Ferrand Collection: Biology and Medicine 22 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

162

E-Print Network 3.0 - akv virus gene Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

44 Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea Summary: that are unexpected for a...

163

E-Print Network 3.0 - archaeal fuselloviridae viruses Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Source: Ecole Polytechnique, Centre de mathmatiques Collection: Mathematics 31 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

164

E-Print Network 3.0 - acidianus bottle-shaped virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

d Source: Ecole Polytechnique, Centre de mathmatiques Collection: Mathematics 3 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

165

E-Print Network 3.0 - acidianus filamentous virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

... Source: Ecole Polytechnique, Centre de mathmatiques Collection: Mathematics 3 Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins...

166

In vitro studies of single-stranded RNA virus self-assembly.  

E-Print Network [OSTI]

??Viruses are self-replicating nucleoprotein complexes that have the ability to cause diseases and are composed of, at least, a single copy of genetic material (either (more)

Comas Garcia, Mauricio

2013-01-01T23:59:59.000Z

167

E-Print Network 3.0 - anti-virus business optimal Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

. Trademarks Symantec, the Symantec logo, Norton AntiVirus, and LiveUpdate are U.S registered ... Source: Khatiwala, Samar - Lamont-Doherty Earth Observatory, Columbia...

168

E-Print Network 3.0 - avian encephalomyelitis virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

age determination of nestling Brown-headed Cowbirds. Wilson Bull. Summary: . A simulation model for the vector-host transmission system of a mosquito-borne avian virus,...

169

Genetic modification of alternative respiration in Nicotiana benthamiana affects basal and salicylic acid-induced resistance to potato virus X  

E-Print Network [OSTI]

constitutively expressing a functional RNA-dependent RNA polymerase (MtRDR1). Unexpectedly, Aox-transgenic plants infected with potato virus X (PVX) showed markedly increased susceptibility to systemic disease induction and virus accumulation in inoculated...

Lee, Wing-Sham; Fu, Shih-Feng; Verchot-Lubicz, Jeanmarie; Carr, John P

2011-02-28T23:59:59.000Z

170

Chlorella Virus PBCV-1 Encodes a Functional Homospermidine Synthase Annette Kaiser,*,1  

E-Print Network [OSTI]

Chlorella Virus PBCV-1 Encodes a Functional Homospermidine Synthase Annette Kaiser,*,1 Michael 16, 1999 Sequence analysis of the 330-kb genome of chlorella virus Paramecium bursaria chlorella uninfected and PBCV-1-infected chlorella, as well as PBCV-1 virions, contain homospermidine, along

Graves, Michael V.

171

Chlorella Virus PBCV-1 Encodes Functional Glutamine: Fructose-6-Phosphate Amidotransferase and UDP-Glucose Dehydrogenase Enzymes  

E-Print Network [OSTI]

Chlorella Virus PBCV-1 Encodes Functional Glutamine: Fructose-6-Phosphate Amidotransferase and UDP August 18, 1998 DNA sequence analysis of the 330-kb Chlorella virus PBCV-1 genome unexpectedly revealed the Chlorella viruses. The products of the reactions catalyzed by these two enzymes are precursors

Graves, Michael V.

172

An Avian Influenza Virus from Waterfowl in South America Contains Genes from North American Avian and Equine Lineages  

E-Print Network [OSTI]

An Avian Influenza Virus from Waterfowl in South America Contains Genes from North American Avian in 2002, there have been few reports of avian influenza in South America. However, surveillance in free to an equine influenza virus from South America. RESUMEN. Un virus de influenza aviar aislado de aves acua

McCracken, Kevin G.

173

Environmental assessment of curly top virus control in California  

SciTech Connect (OSTI)

Curly top is a viral disease of sugarbeets, tomatoes, melons, peppers, beans, cucumbers, squash, pumpkins, spinach, vineseed and other commercially important crops, including ornamentals. Curly top not only infects commercial crops, but at times devastates backyard vegetable and flower gardens. The only known vector of curly top is Circulifer tenellus, commonly known as the beet leafhopper. Control of the beet leafhopper may take place at various locations throughout the San Joaquin Valley, Salinas Valley, Cuyama Valley, Antelope Valley of northern Los Angeles County, Riverside County and the Imperial Valley (See Maps in Appendix ``E``), including portions of Merced, Fresno, Kings. Kern, San Luis Obispo, Monterey, San Benito, Santa Barbara, Los Angeles, Riverside and Imperial Counties. The size of the control program is totally dependent on the location, size, nd distribution of the beet leafhopper population. In a year with a low population, only 80,000 acres in western Fresno, Kings, and Kern Counties may require treatment. In a drought year, the treatment required may increase to more than 200,000 acres and include some inter-coastal valleys of Monterey, San Luis Obispo and Santa Barbara Counties. Not all areas require treatment on an annual basis and at no time is the entire area subject to blanket treatment. The Curly Top Virus Control Program (CTVCP) was instrumental in supporting research which developed an antisera allowing the use of Enzyme-Linked Immunosorbent Assay to determine the presence, on a percentage basis, of curly top virus. Thus, the amount of virus found in a given area lends weight to treatment priorities.

Not Available

1991-03-15T23:59:59.000Z

174

Inhibition of lytic infection of pseudorabies virus by arginine depletion  

SciTech Connect (OSTI)

Pseudorabies virus (PRV) is a member of Alphahepesviruses; it is an enveloped virus with a double-stranded DNA genome. Polyamines (such as spermine and spermidine) are ubiquitous in animal cells and participate in cellular proliferation and differentiation. Previous results of our laboratory showed that the PRV can accomplish lytic infection either in the presence of exogenous spermine (or spermidine) or depletion of cellular polyamines. The amino acid arginine is a precursor of polyamine biosynthesis. In this work, we investigated the role of arginine in PRV infection. It was found that the plaque formation of PRV was inhibited by arginase (enzyme catalyzing the conversion of arginine into ornithine and urea) treatment whereas this inhibition can be reversed by exogenous arginine, suggesting that arginine is essential for PRV proliferation. Western blotting was conducted to study the effect of arginine depletion on the levels of structural proteins of PRV in virus-infected cells. Four PRV structural proteins (gB, gE, UL47, and UL48) were chosen for examination, and results revealed that the levels of viral proteins were obviously reduced in long time arginase treatment. However, the overall protein synthesis machinery was apparently not influenced by arginase treatment either in mock or PRV-infected cells. Analyzing with native gel, we found that arginase treatment affected the mobility of PRV structural proteins, suggesting the conformational change of viral proteins by arginine depletion. Heat shock proteins, acting as molecular chaperons, participate in protein folding and translocation. Our results demonstrated that long time arginase treatment could reduce the expression of cellular heat shock proteins 70 (hsc70 and hsp70), and transcriptional suppression of heat shock protein 70 gene promoter was one of the mechanisms involved in this reduced expression.

Wang, H.-C. [Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan (China); Kao, Y.-C. [Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan (China); Chang, T-J. [Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan (China); Wong, M.-L. [Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung 402, Taiwan (China)]. E-mail: mlwong@dragon.nchu.edu.tw

2005-08-26T23:59:59.000Z

175

Mutation, purification and chemical studies on the tobacco necrosis virus  

E-Print Network [OSTI]

after extraction from frozen infected cowpea leaves by centrifugation for 30 minutes at 16,000 x G. in the Servall centrifuge. The clear, brownish supernatant was treated as outlined in the experiment below. Forty ml of a 0.16 M solution of sodium..., and the resulting lesions were examined* Effect of Environmental Factors on the Virus* Thermal inactivation points* The thermal inactivation point was determined for three strains: II, 1 1 ^ and IV in infectious juice clarified by centrifugation at 16,000 x G...

Hockaday, William Don

1957-01-01T23:59:59.000Z

176

LINGUISTIC ANALYSIS OF THE NUCLEOPROTEIN GENE OF INFLUENZA A VIRUS  

SciTech Connect (OSTI)

We applied linguistic analysis approach, specifically N-grams, to classify nucleotide and amino acids sequences of nucleoprotein (NP) gene of the Influenza A virus isolated from a range of hosts and geographic regions. We considered letter frequency (1-grams), letter pairs frequency (2-grams) and triplets' frequency (3-grams). Classification trees based on 1,2,3-grams variables were constructed for the same NP nucleotide and amino acids strains and their classification efficiency were compared with the clustering obtained using phylogenetic analysis. The results have shown that disregarding positional information for a NP gene can provide the same level of recognition accuracy like alternative more complex classification techniques.

A. SKOURIKHINE; T. BURR

2000-05-01T23:59:59.000Z

177

Contact with dogs, canine distemper virus, and multiple sclerosis  

E-Print Network [OSTI]

to 1945. It was further suggested that the occupation was accompanied by an influx of canine distemper virus through military dogs (44). In Sitka, Alaska, an increase in MS incidence also appeared to correlate with a previous epidemic of CDV... anti-human IgG heavy and light chain secondary antibody (Jackson ImmunoResearch Laboratories Inc, West Grove, PA) was used at a dilution of I:600. Each serum sample was run in four wells, and the average absorbance of the four replicates was used...

Hutka, Marcus Dietrich

1999-01-01T23:59:59.000Z

178

Chlorella viruses encode most, if not all, of the machinery to glycosylate their glycoproteins independent of the endoplasmic reticulum and Golgi  

E-Print Network [OSTI]

Review Chlorella viruses encode most, if not all, of the machinery to glycosylate 2009 Available online 3 August 2009 Keywords: Chlorella viruses PBCV-1 Virus, the large dsDNA-containing chlorella viruses encode most, if not all, of the components to glycosylate

Graves, Michael V.

179

Die Dynamik magnetischer Flu rohren im Sonnen eck  

E-Print Network [OSTI]

with the photosphere, moves inward towards the umbra. In the upper part of the convection zone an up ow along the tube to 14km/s. The hot, hence bright footpoint, which moves radially inwards towards the umbra, can

Schlichenmaier, Rolf

180

varicose veins smoking obesity swine flu high blood pressure  

E-Print Network [OSTI]

blood pressu #12;The records of your health, lifestyle and health habits (your data) already make a huge

Diggle, Peter J.

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Information From Your Health Care ProviderGASTROENTERITIS (Stomach Flu)  

E-Print Network [OSTI]

are bacterial or parasitic infections, food- borne toxins, shellfish and marine animal poisoning, food an object that has germs on it. Contaminated food or water is another source for infection. · Other causes, or antacids. · Contaminated food or water. · Travel to foreign countries. PREVENTIVE MEASURES · No specific

Rusu, Adrian

182

Preventing the Flu Good Health Habits Can Help Stop Germs  

E-Print Network [OSTI]

mouth and nose. When coughing or sneezing, cover your mouth and nose with a tissue or your inside elbow

Rhode Island, University of

183

Swine Flu and Common Infections to Prepare For  

E-Print Network [OSTI]

particles can travel up to 12 feet through the air when someone with a cold coughs or sneezes http symptoms: ­ Headache or pressure on the face ­ Cough ­ Stuffy nose Nose drainage ("snots") or phlegm (rest do not require antibiotics) ­ Fever, swollen lymph nodes, no cough, white spots on tonsils

Goldman, Steven A.

184

BACTERIAL MENINGITIS AND SWINE FLU THE DIFFERENCES BETWEEN THE SYMPTOMS  

E-Print Network [OSTI]

bright lights stiff neck cough sore throat body aches runny nose congestion fatigue If you think you have

Oxford, University of

185

ORISE: Pandemic Flu Toolkits | How ORISE is Making a Difference  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas Conchas recoveryLaboratory |CHEMPACK MappingHistory The OakMentorExercisesof

186

Point-of-Care Flu Diagnosis | GE Global Research  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administration the1 - September 2006Photovoltaic TheoryPlant 242-Z Americiumand InDevR Scientists

187

FLU5A425 | netl.doe.gov  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsing ZirconiaPolicy andExsolutionFES Committees of9, 2011COMMERCIALBulletin

188

Fast pandemic detection tool ready to fight flu  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series toESnet4:Epitaxial ThinFORFALL NEWS ROCKY MOUNTAINFast pandemic detection

189

Cold and Flu Prevention - HPMC Occupational Health Services  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed New SubstationClean Communities ofCellulosic Feedstock -Import Costs forForms:0

190

Pandemic Flu Planning | Y-12 National Security Complex  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas Conchas recoveryLaboratorySpeedingOptimizingToolsto control intensePage Not

191

Structure and Receptor Specificity of an Avian Flu Antigen  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBiSiteNeutronStrategicOur Mission

192

Structure and Receptor Specificity of an Avian Flu Antigen  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBiSiteNeutronStrategicOur MissionStructure and Receptor Specificity

193

Structure and Receptor Specificity of an Avian Flu Antigen  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBiSiteNeutronStrategicOur MissionStructure and Receptor

194

Structure and Receptor Specificity of an Avian Flu Antigen  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBiSiteNeutronStrategicOur MissionStructure and ReceptorStructure and

195

Structure and Receptor Specificity of an Avian Flu Antigen  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas ConchasPassiveSubmittedStatus TomAbout » Staff125,849| OSTI, USStructure

196

Identification of Novel Positive-Strand RNA Viruses by Metagenomic Analysis of Archaea-Dominated Yellowstone Hot Springs  

SciTech Connect (OSTI)

There are no known RNA viruses that infect Archaea. Filling this gap in our knowledge of viruses will enhance our understanding of the relationships between RNA viruses from the three domains of cellular life and, in particular, could shed light on the origin of the enormous diversity of RNA viruses infecting eukaryotes. We describe here the identification of novel RNA viral genome segments from high-temperature acidic hot springs in Yellowstone National Park in the United States. These hot springs harbor low-complexity cellular communities dominated by several species of hyperthermophilic Archaea. A viral metagenomics approach was taken to assemble segments of these RNA virus genomes from viral populations isolated directly from hot spring samples. Analysis of these RNA metagenomes demonstrated unique gene content that is not generally related to known RNA viruses of Bacteria and Eukarya. However, genes for RNA-dependent RNA polymerase (RdRp), a hallmark of positive-strand RNA viruses, were identified in two contigs. One of these contigs is approximately 5,600 nucleotides in length and encodes a polyprotein that also contains a region homologous to the capsid protein of nodaviruses, tetraviruses, and birnaviruses. Phylogenetic analyses of the RdRps encoded in these contigs indicate that the putative archaeal viruses form a unique group that is distinct from the RdRps of RNA viruses of Eukarya and Bacteria. Collectively, our findings suggest the existence of novel positive-strand RNA viruses that probably replicate in hyperthermophilic archaeal hosts and are highly divergent from RNA viruses that infect eukaryotes and even more distant from known bacterial RNA viruses. These positive-strand RNA viruses might be direct ancestors of RNA viruses of eukaryotes.

Benjamin Bolduc; Daniel P. Shaughnessy; Yuri I. Wolf; Eugene V. Koonin; Francisco F. Roberto; Mark Young

2012-05-01T23:59:59.000Z

197

The grapefruit flavonoid naringenin as a Hepatitis C virus therapy : efficacy, mechanism and delivery  

E-Print Network [OSTI]

Hepatitis C virus (HCV) infection accounts for approximately 40% of chronic liver disease in the United States and results in an estimated 8,000-10,000 deaths annually. Simulations suggest that in the next decade morbidity ...

Goldwasser, Jonathan

2010-01-01T23:59:59.000Z

198

Multiplication of soilborne wheat mosaic virus (SBWMV) in wheat roots infected by a soil  

E-Print Network [OSTI]

, Fortuna, Moulitt, Pernel, Sabre, Tarasque. Sabre and Avalon showed the same virus concentration as Hardi 33 cultivars étudiés, 9 d'entre eux : « Capitole, Cargo, Fortuna, Moulin, Rescler, Albatros, Fidel

Paris-Sud XI, Université de

199

Virus constructed iron phosphate lithium ion batteries in unmanned aircraft systems  

E-Print Network [OSTI]

FePO? lithium ion batteries that have cathodes constructed by viruses are scaled up in size to examine potential for use as an auxiliary battery in the Raven to power the payload equipment. These batteries are assembled ...

Kolesnikov-Lindsey, Rachel

200

E-Print Network 3.0 - alfalfa mosaic virus-mediated Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Search Powered by Explorit Topic List Advanced Search Sample search results for: alfalfa mosaic virus-mediated Page: << < 1 2 3 4 5 > >> 1 Alfalfa Production Guide for the...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

E-Print Network 3.0 - al virus sincitial Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Search Powered by Explorit Topic List Advanced Search Sample search results for: al virus sincitial Page: << < 1 2 3 4 5 > >> 1 Temporal trends in the discovery of human...

202

E-Print Network 3.0 - al virus leucemia Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Search Powered by Explorit Topic List Advanced Search Sample search results for: al virus leucemia Page: << < 1 2 3 4 5 > >> 1 EnfErmEdadEs dEl jaguar En Estado silvEstrE...

203

Extensive investigation of reticuloendotheliosis virus in the endangered Attwater's prairie chicken  

E-Print Network [OSTI]

to the repopulation of the Attwater's prairie chicken. Several aspects of virus infection were investigated. Reagents capable of recognizing prairie chicken IgY and viral gag polypeptides were developed for use in assays for detection of antibody responses...

Bohls, Ryan Lanier

2007-09-17T23:59:59.000Z

204

Mathematical modelling and analysis of replicated viruses for glioma cell control  

E-Print Network [OSTI]

- veloped, with oncolytic viruses emerging as an innovative therapeutic tool 1 UMR 077 Plant Pathology on model parameters which guarantee the permanence of the system and the existence of periodic solutions

Boyer, Edmond

205

E-Print Network 3.0 - andes virus chi-7913 Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Ecology 20 1074 PHYTOPATHOLOGY Genetics and Resistance Summary: : Resistance to Potato virus Y in Wild Relatives of the Cultivated Potato X. K. Cai, D. M. Spooner, and S....

206

Engineering and targeting glycan receptor binding of influenza A virus hemagglutinin  

E-Print Network [OSTI]

The critical first step in the host infection by influenza A virus is the binding of the viral surface glycoprotein hemagglutinin (HA) to the sialylated glycan receptors terminated by N-acetyineuraminic acid (Neu5Ac) ...

Jayaraman, Akila

2011-01-01T23:59:59.000Z

207

Viruses 2013, 5, 1292-1324; doi:10.3390/v5051292 ISSN 1999-4915  

E-Print Network [OSTI]

of Liver Disease Daniel J. Felmlee 1,2 , Mohamed Lamine Hafirassou 1,2 , Mathieu Lefevre 1,2 , Thomas F: Hepatitis C virus; lipoproteins; apolipoproteins; apoE; apoB; cholesterol; triglyceride; viral attachment

Paris-Sud XI, Université de

208

The role of mast cells in Theiler's virus-induced demyelination  

E-Print Network [OSTI]

Mast cells within the peripheral and central nervous systems of CBA and SJL mice during Theiler's virus-induced demyelination (TVID) have been investigated. CBA mice are intermediately susceptible and SJL mice are highly susceptible to TVIID...

Dean, Dana D

1997-01-01T23:59:59.000Z

209

Field distribution and genetic variability of Panicum mosaic virus satellite RNAs in St. Augustine decline  

E-Print Network [OSTI]

. . . . . . . . . . . 20 Introduction . . Materials and Methods. . . Results. . . . . . . . . . Discussion. . . . 20 . 21 . 24 . 29 111 GENETIC DIVERSITY OF PANICUM MOSAIC VIRUS SATELLITE RNAs IN ST. AUGUSTINEGRASS. . 32 Introduction . . Materials and Methods...RNA is found associated with Australian isolates of BYDV-RPV. This non- coding satRNA is undetectable in the field and becomes perceptible only following greenhouse propagation. This satRNA attenuates the symptoms induced by the helper virus (BYDV-RPV) alone...

Cabrera Perez, Over

1999-01-01T23:59:59.000Z

210

Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine  

E-Print Network [OSTI]

of MASTER OF SCIENCE Approved by: Chair of Committee, Blanca M. Lupiani Committee Members, Sanjay M. Reddy Michael Criscitiello Head of Department, Linda Logan August 2012 Major Subject: Biomedical Sciences iii ABSTRACT Evaluation... of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine. (August 2012) Christine Nguyen Vuong, B.S., Texas A&M University Chair of Advisory Committee: Dr. Blanca M. Lupiani Although avian influenza virus (AIV) infections in domestic poultry...

Vuong, Christine

2012-10-19T23:59:59.000Z

211

Effects of method of wheat streak mosaic virus transmission on the resistance of selected hosts  

E-Print Network [OSTI]

of these hybrids, which showed resistance to infection, were frequently reclassified as a susceptible host after reinoculation. The efficiency of transmission of WSMV to wheat by the mite, artist's airbrush, and carborundum rub inoculations were 49. 2, and 41... OF CONTENTS Chapter Page I INTRODUCTION II REVIEW OF THE LITERATURE III GENERAL MATERIALS AND METHODS Plant Materials Virus and Vector Virus Mite Transmission Carborundum rub Artist's airbrush Mite transmission 10 10 11 11 11 1. 2 12 12 13...

Cho, Han Yong

1969-01-01T23:59:59.000Z

212

Clinical mite infestations of domestic cats seropositive for feline immunodeficiency and/or feline leukemia viruses  

E-Print Network [OSTI]

CLINICAL MITE INFESTATIONS OF DOMESTIC CATS SEROPOSITIVE FOR FELINE ~ODEFICIENCY AND/OR FELINE LEUKEMIA VIRUSES A Thesis by CHRISTIAN ERIC SIMMONS Submitted to the OIIlce of Graduate Studies of Texas AkM University in partial fulfdlment... of the requirements for the degree of MASTER OF SCIENCE August 2000 Major Subject: Entomology CLINICAL MITE INFESTATIONS OF DOMESTIC CATS SEROPO SITIVE FOR FELINE ~ODEFICIENCY AND/OR FELINE LEUKEMA VIRUSES A Thesis by CHRISTIAN ERIC SIMMONS Submitted to Texas...

Simmons, Christian Eric

2000-01-01T23:59:59.000Z

213

Caractrisation d'une nouvelle souche du virus du fltrissement de la fve isole de l'artichaut  

E-Print Network [OSTI]

° 256, Université de Rennes I, F35042 Rennes Cedex R?SUM? Un virus isolé de l'artichaut « Camus de in France. A virus isolated from artichoke "Camus de Bretagne" in France has been characterized biologically « Camus de Bretagne» (MIGLIORI et al., 1987). Deux virus sur les 6 observés sont fréquents ; il s'agit d

Paris-Sud XI, Université de

214

Production of glycoprotein-deleted rabies viruses for monosynaptic tracing and high-level gene expression in neurons  

E-Print Network [OSTI]

Recombinant rabies viruses rendered replication-deficient by the deletion of their envelope glycoprotein gene are useful tools for neuroscientists, permitting (1) extraordinarily high transgene expression levels within ...

Wickersham, Ian R.

215

The Structure of a T=169d Algal Virus, PBCV-1, at 15 Resolution ** V. Bowman,* N. H. Olson,*,  

E-Print Network [OSTI]

Pathology, University of Nebraska, Lincoln, NE 68583-0722 Paramecium bursaria chlorella virus 1 (PBCV-1) (genus Chlorovirus, family Phycodnaviridae), infects certain unicellular, exsymbiotic, chlorella

Baker, Timothy S.

216

Differential cytokine mRNA expression induced by binding of virulent and avirulent molecularly cloned equine infectious anemia viruses to equine macrophages  

E-Print Network [OSTI]

Equine infectious anemia virus (EIAV) causes rapid development of acute disease followed by recurring episodes of fever, thrombocytopenia and viremia, termed chronic EIA. Most infected horses control the virus by immune mechanisms and become...

Lim, Wah-Seng

2004-11-15T23:59:59.000Z

217

Targeting Sindbis virus-based vectors to Fc receptor-positive cell types  

SciTech Connect (OSTI)

Some viruses display enhanced infection for Fc receptor (FcR)-positive cell types when complexed with virus-specific immunoglobulin (Ig). This process has been termed antibody-dependent enhancement of viral infection (ADE). We reasoned that the mechanism of ADE could be exploited and adapted to target alphavirus-based vectors to FcR-positive cell types. Towards this goal, recombinant Sindbis viruses were constructed that express 1 to 4 immunoglobulin-binding domains of protein L (PpL) as N-terminal extensions of the E2 glycoprotein. PpL is a bacterial protein that binds the variable region of antibody kappa light chains from a range of mammalian species. The recombinant viruses incorporated PpL/E2 fusion proteins into the virion structure and recapitulated the species-specific Ig-binding phenotypes of native PpL. Virions reacted with non-immune serum or purified IgG displayed enhanced binding and ADE for several species-matched FcR-positive murine and human cell lines. ADE required virus expression of a functional PpL Ig-binding domain, and appeared to be Fc{gamma}R-mediated. Specifically, ADE did not occur with Fc{gamma}R-negative cells, did not require active complement proteins, and did not occur on Fc{gamma}R-positive murine cell lines when virions were bound by murine IgG-derived F(ab'){sub 2} fragments.

Klimstra, William B. [Department of Microbiology and Immunology, Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, LA 71130 (United States); Williams, Jacqueline C. [Department of Biology, University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249-0662 (United States); Ryman, Kate D. [Department of Microbiology and Immunology, Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, LA 71130 (United States); Heidner, Hans W. [Department of Biology, University of Texas at San Antonio, 6900 North Loop 1604 West, San Antonio, TX 78249-0662 (United States)]. E-mail: hans.heidner@utsa.edu

2005-07-20T23:59:59.000Z

218

The effects of Moloney Sarcoma Virus infection and subsequent thymosin administration on the functional thymus-dependent lymphocyte populations of BALB/C and congenitally athymic nude mice  

E-Print Network [OSTI]

. Congenitally athymic nude mice were kindly suppli. ed by Dr Gary B. Thurman, Galveston, Texas. BALB/c and Swiss?Webster mice were maintained on laboratory chow and water ad libitum until use. Virus. The virus used was Moloney Sarcoma Virus, originally...THE EFFECTS OF MOLONEY SARCOMA VIRUS INFECTION AND SUBSEQUENT THYMOSIN ADMINISTRATION ON THE FUNCTIONAL THYMUS-DEPENDENT LYMPHOCYTE POPULATIONS OF BALB/C AND CONGENITALLY ATHYMIC NUDE MICE A Thesis by GAILEN DAUGHERTY MARSHALL, JR. Submitted...

Marshall, Gailen Daughterty

1975-01-01T23:59:59.000Z

219

Structure of Epstein-Barr Virus Glycoprotein 42 Suggests a Mechanism for Triggering Receptor-Activated Virus Entry  

SciTech Connect (OSTI)

Epstein-Barr virus requires glycoproteins gH/gL, gB, and gp42 to fuse its lipid envelope with B cells. Gp42 is a type II membrane protein consisting of a flexible N-terminal region, which binds gH/gL, and a C-terminal lectin-like domain that binds to the B-cell entry receptor human leukocyte antigen (HLA) class II. Gp42 triggers membrane fusion after HLA binding, a process that requires simultaneous binding to gH/gL and a functional hydrophobic pocket in the lectin domain adjacent to the HLA binding site. Here we present the structure of gp42 in its unbound form. Comparisons to the previously determined structure of a gp42:HLA complex reveals additional N-terminal residues forming part of the gH/gL binding site and structural changes in the receptor binding domain. Although the core of the lectin domain remains similar, significant shifts in two loops and an {alpha} helix bordering the essential hydrophobic pocket suggest a structural mechanism for triggering fusion.

Kirschner, Austin N.; Sorem, Jessica; Longnecker, Richard; Jardetzky, Theodore S.; (NWU); (Stanford-MED)

2009-05-26T23:59:59.000Z

220

Biochemistry 1995, 34, 4077-4087 4077 C2, an Unusual Filamentous Bacterial Virus: Protein Sequence and Conformation,  

E-Print Network [OSTI]

-fold screw, the symmetry being designated CsS-2. In class I1 viruses (Pf1, Xf, and PB), the protein helices have approximately 5.4-fold screw symmetry, nominally ClS-5.4. The subunits in successive levels and Day (1988)l. Such viruses are assembled at the host cell membrane as they are extruded into the medium

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

I Ph}",l. 28, 846-850 (1992) REPLICATION OF CHLORELLA VIRUS PBCV-l AND HOST KARYOTYPE  

E-Print Network [OSTI]

I Ph}",l. 28, 846-850 (1992) REPLICATION OF CHLORELLA VIRUS PBCV-l AND HOST KARYOTYPE DETERMINATION 97331-2902 ABSTRACT We demonstrated the time-courseojDNA rePlication of the 330-kbp Chlorella virus PBCV the sizes oj the bands predicts a genome size oj 39-45 Mbp afDNA. Key index words: Chlorella; Chloro

Graves, Michael V.

222

HEPATITIS C VIRUS (HCV) PROTEINS INDUCE LIPOGENESIS AND DEFECTIVE TRIGLYCERIDE SECRETION IN TRANSGENIC MICE EXPRESSING THE HCV FULL-  

E-Print Network [OSTI]

1 HEPATITIS C VIRUS (HCV) PROTEINS INDUCE LIPOGENESIS AND DEFECTIVE TRIGLYCERIDE SECRETION, Université Paris 12, Créteil, France4 Running head: Lipogenesis and triglyceride secretion in HCV mice hepatitis C virus (HCV) infection is associated with altered lipid metabolism and hepatocellular steatosis

Paris-Sud XI, Université de

223

Prevalence of Antibodies against Avian Influenza A (H5N1) Virus among Cullers and Poultry Workers in Ho  

E-Print Network [OSTI]

Prevalence of Antibodies against Avian Influenza A (H5N1) Virus among Cullers and Poultry Workers, highly pathogenic avian influenza A (H5N1) viruses caused large scale outbreaks in poultry in the Ho Chi Minh City area in Vietnam. We studied the prevalence of antibodies against H5N1 in poultry workers

Paris-Sud XI, Université de

224

Stochastic dynamics of virus capsid formation: direct versus hierarchical self-assembly  

E-Print Network [OSTI]

In order to replicate within their cellular host, many viruses have developed self-assembly strategies for their capsids which are sufficiently robust as to be reconstituted in vitro. Mathematical models for virus self-assembly usually assume that the bonds leading to cluster formation have constant reactivity over the time course of assembly (direct assembly). In some cases, however, binding sites between the capsomers have been reported to be activated during the self-assembly process (hierarchical assembly). In order to study possible advantages of such hierarchical schemes for icosahedral virus capsid assembly, we use Brownian dynamics simulations of a patchy particle model that allows us to switch binding sites on and off during assembly. For T1 viruses, we implement a hierarchical assembly scheme where inter-capsomer bonds become active only if a complete pentamer has been assembled. We find direct assembly to be favorable for reversible bonds allowing for repeated structural reorganizations, while hierarchical assembly is favorable for strong bonds with small dissociation rate, as this situation is less prone to kinetic trapping. However, at the same time it is more vulnerable to monomer starvation during the final phase. Increasing the number of initial monomers does have only a weak effect on these general features. The differences between the two assembly schemes become more pronounced for more complex virus geometries, as shown here for T3 viruses, which assemble through homogeneous pentamers and heterogeneous hexamers in the hierarchical scheme. In order to complement the simulations for this more complicated case, we introduce a master equation approach that agrees well with the simulation results.

Johanna E. Baschek; Heinrich C. R. Klein; Ulrich S. Schwarz

2015-01-31T23:59:59.000Z

225

Interplay Between Viral and Cellular Factors Determines the Fate of Herpes Simplex Virus type I Infection  

E-Print Network [OSTI]

are Attenuated for Viral Replication and Impaired for Explant- Induced Reactivation 2.1 Abstract 2.2 Materials and Methods 2.3 Results 2.4 Discussion 2.5 Figures and Figure legends Chapter 3: N-terminal phosphorylation sites of herpes simplex virus 1 ICP0... differentially regulate its activities and enhance viral replication. 3.1 Abstract 3.2 Materials and Methods 3.3 Results 3.4 Discussion 3.5 Tables 3.6 Figures and Figure legends Chapter 4: Two Amino Acid Substitutions in Herpes Simplex Virus 1 ICP6 Impair...

Mabrouk-Mostafa, Heba

2014-05-31T23:59:59.000Z

226

A Patterned 3D Silicon Anode Fabricated by Electrodeposition on a Virus-Structured Current Collector  

SciTech Connect (OSTI)

Electrochemical methods were developed for the deposition of nanosilicon onto a 3D virus-structured nickel current collector. This nickel current collector is composed of self-assembled nanowire-like rods of genetically modified tobacco mosaic virus (TMV1cys), chemically coated in nickel to create a complex high surface area conductive substrate. The electrochemically deposited 3D silicon anodes demonstrate outstanding rate performance, cycling stability, and rate capability. Electrodeposition thus provides a unique means of fabricating silicon anode materials on complex substrates at low cost.

Chen, X L; Gerasopoulos, K; Guo, J C; Brown, A; Wang, Chunsheng; Ghodssi, Reza; Culver, J N

2011-01-01T23:59:59.000Z

227

Evidence for ADAR-induced hypermutation of the Drosophila sigma virus (Rhabdoviridae).  

E-Print Network [OSTI]

, Cambridge CB2 3EH, UK Email: Jennifer A Carpenter* - jenny@jennycarpenter.com; Liam P Keegan - Liam.Keegan@hgu.mrc.ac.uk; Lena Wilfert - lb445@cam.ac.uk; Mary A O'Connell - m.oconnell@hgu.mrc.ac.uk; Francis M Jiggins - f... of RNA editing identified by comparative genomics. Science 2003, 301(5634):832-836. 24. Weber F, Wagner V, Rasmussen SB, Hartmann R, Paludan SR: Dou- ble-Stranded RNA Is Produced by Positive-Strand RNA Viruses and DNA Viruses but Not in Detectable Amounts...

Carpenter, Jennifer A; Keegan, Liam; Wilfert, Lena; O'Connell, Mary A; Jiggins, Francis M

2009-11-26T23:59:59.000Z

228

Ratings of Commercial Grain Sorghum Hybrids to the Maize Dwarf Mosaic Virus.  

E-Print Network [OSTI]

. Cargill 5665 Intermediate 57. Horizon H -840 Tolerant 14. Cargill 6658 Tolerant 58. Horizon H-850 Intermediate 15. Cargill C 55 Susceptible 59. Horizon H -940 Susceptible 16. Cargill C 60 Tolerant 60. Horizon H-95G Tolerant 17. Cargill 0 70... Susceptible 81. Northrup King 2670 Susceptible 44. OeKalb M-565 Intermediate 82. Richardson Y-100 A Tolerant Maize dwarf Maize dwarf Number / company /hybrid mosaic virus Number/company /hybrid mosaic virus 83. Richardson Y-300 D Tolerant 116. Taylor...

Horne, C. Wendell; Frederiksen, Richard A.; Toler, Robert W.; Trampota, Jerry D.

1984-01-01T23:59:59.000Z

229

Characterization of a virus associated with head and lateral line erosion syndrome (HLLE) in marine angelfish  

E-Print Network [OSTI]

culture infective dose (TCIDss) method of determination as outlined by Rovozzo and Burke (1973). Virus titers were determined following 10-fold serial dilutions on RFDF monolayers in tissue culture chamber slides (Lab-Tek, Nunc, Inc. , Naperville, Il... model L-2 ultracentrifuge for 1. 5 hr using a swing-bucket type rotor (SW41 or SW28). The virus pellet was resuspended in 1 ml TNE buffer (0. 025 M Tris [Mallinckrodt, Paris, KY, USA], 0. 1 M NaCl, 1mM EDTA [Sigma Chemical Co. , St. Louis, MO, USA], p...

Varner, Patricia Wilcox

2012-06-07T23:59:59.000Z

230

Potential Geographic Distribution of the Novel Avian-Origin Influenza A (H7N9) Virus  

E-Print Network [OSTI]

, Jiangxi, Fujian and Guangdong provinces in the south (Figure 1). The source of H7N9 human infections is unclear, but it appears to be carried by poultry, in their secretions or excretions [78]. Ecological niche modeling (ENM) offers a means by which...N9 virus through contact with infected poultry or contaminated environments, including markets that sell live poultry [16]. However clear links between infections in poultry and human cases have been difficult to establish, because this virus does...

Zhu, Gengping; Peterson, A. Townsend

2014-04-01T23:59:59.000Z

231

Identification of full-length transmitted/founder viruses and their progeny in primary HIV-1 infection  

SciTech Connect (OSTI)

Identification of transmitted/founder virus genomes and their progeny by is a novel strategy for probing the molecular basis of HIV-1 transmission and for evaluating the genetic imprint of viral and host factors that act to constrain or facilitate virus replication. Here, we show in a cohort of twelve acutely infected subjects (9 clade B; 3 clade C), that complete genomic sequences of transmitted/founder viruses could be inferred using single genome amplification of plasma viral RNA, direct amplicon sequencing, and a model of random virus evolution. This allowed for the precise identification, chemical synthesis, molecular cloning, and biological analysis of those viruses actually responsible for productive clinical infection and for a comprehensive mapping of sequential viral genomes and proteomes for mutations that are necessary or incidental to the establishment of HIV-1 persistence. Transmitted/founder viruses were CD4 and CCR5 tropic, replicated preferentially in activated primary T-Iymphocytes but not monocyte-derived macrophages, and were effectively shielded from most heterologous or broadly neutralizing antibodies. By 3 months of infection, the evolving viral quasispecies in three subjects showed mutational fixation at only 2-5 discreet genomic loci. By 6-12 months, mutational fixation was evident at 18-27 genomic loci. Some, but not all, of these mutations were attributable to virus escape from cytotoxic Tlymphocytes or neutralizing antibodies, suggesting that other viral or host factors may influence early HIV -1 fitness.

Korber, Bette [Los Alamos National Laboratory; Hraber, Peter [Los Alamos National Laboratory; Giorgi, Elena [Los Alamos National Laboratory; Bhattacharya, T [Los Alamos National Laboratory

2009-01-01T23:59:59.000Z

232

Lung Irradiation Increases Mortality After Influenza A Virus Challenge Occurring Late After Exposure  

SciTech Connect (OSTI)

Purpose: To address whether irradiation-induced changes in the lung environment alter responses to a viral challenge delivered late after exposure but before the appearance of late lung radiation injury. Methods and Materials: C57BL/6J mice received either lung alone or combined lung and whole-body irradiation (0-15 Gy). At 10 weeks after irradiation, animals were infected with 120 HAU influenza virus strain A/HKx31. Innate and adaptive immune cell recruitment was determined using flow cytometry. Cytokine and chemokine production and protein leakage into the lung after infection were assessed. Results: Prior irradiation led to a dose-dependent failure to regain body weight after infection and exacerbated mortality, but it did not affect virus-specific immune responses or virus clearance. Surviving irradiated animals displayed a persistent increase in total protein in bronchoalveolar lavage fluid and edema. Conclusions: Lung irradiation increased susceptibility to death after infection with influenza virus and impaired the ability to complete recovery. This altered response does not seem to be due to a radiation effect on the immune response, but it may possibly be an effect on epithelial repair.

Manning, Casey M. [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States)] [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States); Johnston, Carl J. [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States) [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States); Department of Pediatrics, University of Rochester Medical Center, Rochester, New York (United States); Reed, Christina K. [Department of Pediatrics, University of Rochester Medical Center, Rochester, New York (United States)] [Department of Pediatrics, University of Rochester Medical Center, Rochester, New York (United States); Lawrence, B. Paige [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States) [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States); Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York (United States); Williams, Jacqueline P. [Department of Radiation Oncology, University of Rochester Medical Center, Rochester, New York (United States)] [Department of Radiation Oncology, University of Rochester Medical Center, Rochester, New York (United States); Finkelstein, Jacob N., E-mail: Jacob_Finkelstein@urmc.rochester.edu [Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States); Department of Pediatrics, University of Rochester Medical Center, Rochester, New York (United States); Department of Radiation Oncology, University of Rochester Medical Center, Rochester, New York (United States)

2013-05-01T23:59:59.000Z

233

Rapid emergence of hepatitis C virus protease inhibitor resistance is expected  

SciTech Connect (OSTI)

Approximately 170 million people worldwide are infected with hepatitis C virus (HCV). Current therapy, consisting of pegylated interferon (PEG-IFN) and ribavirin (RBV), leads to sustained viral elimination in only about 45% of patients treated. Telaprevir (VX-950), a novel HCV NS3-4A serine protease inhibitor, has demonstrated substantial antiviral activity in patients with chronic hepatitis C genotype 1 infection. However, some patients experience viral breakthrough during dosing, with drug resistant variants being 5%-20% of the virus population as early as day 2 after treatment initiation. Why viral variants appear such a short time after the start of dosing is unclear, especially since this has not been seen with monotherapy for either human immunodeficiency virus or hepatitis B virus. Here, using a viral dynamic model, we explain why such rapid emergence of drug resistant variants is expected when potent HCV protease inhibitors are used as monotherapy. Surprisingly, our model also shows that such rapid emergence need not be the case with some potent HCV NS5B polymerase inhibitors. Examining the case of telaprevir therapy in detail, we show the model fits observed dynamics of both wild-type and drug-resistant variants during treatment, and supports combination therapy of direct antiviral drugs with PEG-IFN and/or RBV for hepatitis C.

Rong, Libin [Los Alamos National Laboratory; Perelson, Alan S [Los Alamos National Laboratory; Ribeiro, Ruy M [Los Alamos National Laboratory

2009-01-01T23:59:59.000Z

234

Resource dynamics during infection of Micromonas pusilla by virus MpV-Sp1  

E-Print Network [OSTI]

loop (Fuhrman, 1999). Large-scale processes such as carbon sequestration into the deep ocean dependency of lysis. Introduction Phytoplankton are important components of global carbon cycling, accounting infected, suggest that viruses channel between 6% and 26% of photosynthetically fixed carbon to the DOM

Lawrence, J.E.

235

Quantitative analysis of virus and plasmid trafficking in cells Thibault Lagache, Emmanuel Dauty  

E-Print Network [OSTI]

Intracellular transport of DNA carriers is a fundamental step of gene delivery. By combining both theoretical, the geometry of cell and nucleus, the number and sizes of nuclear pores, the various degradation factors to be necessary in the case of viruses. A recent study [8] showed that microtubules shape the distribution

Lagache, Thibault

236

Nuclear import of hepatitis B virus capsids and release of the viral genome  

E-Print Network [OSTI]

Nuclear import of hepatitis B virus capsids and release of the viral genome Birgit Rabe*, Angelika through the nuclear pore into the nuclear basket. Import depended on phosphorylation of the capsid protein from the inner face of the nuclear pore. Immature capsids that did not contain the mature viral genome

Panté, Nelly

237

Evaluation of Performance in Yearling Crossbred Steers following Bovine Viral Diarrhea Virus Challenge  

E-Print Network [OSTI]

Virus (BVDV) challenge. Yearling, F2 and F3 Nellore-Angus steers (n = 380) from the Texas A&M McGregor Genomics herd were utilized over 4 years, and were stratified by sire over three vaccine groups of modified-live (MLV), killed (KV), and non...

Runyan, Chase Anthony

2013-10-31T23:59:59.000Z

238

A C. elegans-based foam for rapid on-site detection of residual live virus.  

SciTech Connect (OSTI)

In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

2012-02-01T23:59:59.000Z

239

Risk Factors and Characteristics of Low Pathogenic Avian Influenza Virus Isolated from Commercial Poultry in  

E-Print Network [OSTI]

Poultry in Tunisia Wafa Tombari1 , Mathilde Paul2,3 , Jihene Bettaieb4 , Imen Larbi1 , Jihene Nsiri1 the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors vaccination of all poultry flocks with continuous monitoring of poultry stations may ensure reduction of AIV

Paris-Sud XI, Université de

240

To identify tickborne viruses circulating in Kenya and the surrounding region, we conducted surveillance at abat-  

E-Print Network [OSTI]

To identify tickborne viruses circulating in Kenya and the surrounding region, we conducted surveillance at abat- toirs in Nairobi, Kenya. Species of ticks collected included Rhipicephalus pulchellus (56 the abundance of tick- borne arboviruses in Kenya and the surrounding region, we collected and tested ticks

Wang, David

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Introduction Modified viruses are becoming the most commonly used vectors in gene therapy. The viral  

E-Print Network [OSTI]

. The chromatography protocol was developed at small- scale using RESOURCETM Q, a 1 ml column pre-packed with SOURCE 15 of a high transduction yield. Traditionally, purification of virus/viral vectors to high purities is done-step anion exchange chromatography purification on SOURCETM 15Q is a very attractive alternative to gradient

Lebendiker, Mario

242

Telomerase Regulation and Telomere Maintenance during Human T-cell Leukemia/Lymphoma Virus (HTLV-I) Transformation  

E-Print Network [OSTI]

Human T-cell leukemia/lymphoma virus (HTLV-I) is the etiological agent of the lymphoproliferative disorder, adult T-cell leukemia/lymphoma (ATLL) and the neurodegenerative disease, tropical spastic paraparesis/HTLV-I-associated ...

Bellon, Marcia Lynn

2008-04-29T23:59:59.000Z

243

Ocean Viruses: Tiny entities with Global Impacts ( JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)  

SciTech Connect (OSTI)

Matt Sullivan from the University of Arizona on "Ocean Viruses: Tiny Entities with Global Impacts" at the 7th Annual Genomics of Energy & Environment Meeting on March 22, 2012 in Walnut Creek, Calif.

Sullivan, Matthew B [University of Arizona] [University of Arizona

2012-03-22T23:59:59.000Z

244

Circulation of Different Lineages of Dengue Virus 2, Genotype American/Asian in Brazil: Dynamics and Molecular and Phylogenetic Characterization  

E-Print Network [OSTI]

The American/Asian genotype of Dengue virus type 2 (DENV-2) was introduced into the Americas in the 80?s. Although there is no data showing when this genotype was first introduced into Brazil, it was first detected in ...

Drumond, Betnia Paiva

245

Association of Tonate Virus (Subtype IIIB of the Venezuelan Equine Encephalitis Complex) with Encephalitis in a Human  

E-Print Network [OSTI]

the VEE complex, since no virus could be recovered from clinical specimens cultured on mosquito cells) was first isolated in French Gui- ana, an overseas French department located between Brazil and Surinam

Paris-Sud XI, Université de

246

Ocean Viruses: Tiny entities with Global Impacts ( JGI Seventh Annual User Meeting 2012: Genomics of Energy and Environment)  

ScienceCinema (OSTI)

Matt Sullivan from the University of Arizona on "Ocean Viruses: Tiny Entities with Global Impacts" at the 7th Annual Genomics of Energy & Environment Meeting on March 22, 2012 in Walnut Creek, Calif.

Sullivan, Matthew B [University of Arizona

2013-01-15T23:59:59.000Z

247

Post-exposure prophylaxis of dogs experimentally exposed to rabies street virus using an inactivated tissue-culture vaccine  

E-Print Network [OSTI]

of structures rather than one molecular structure, making it difficult to evaluate the mechanism of 15 plasma membrane fusion. Iwaski studied the early events 13 of rabies virus invasion of BHK-21 cells and found that within 5 to 30 minutes, the virus...-infected dogs were submitted to Monoclonal Antibody testing for strain identification. Serum samples from all 3 groups were tested by the Rapid Fluorescent Focus Inhibition Test (RFFIT) for detection of serum neutralizing antibody levels. The results...

Layton, Randal Cleo

1988-01-01T23:59:59.000Z

248

A screen for genetic suppressor elements of hepatitis C virus identifies a supercharged protein inhibitor of viral replication  

E-Print Network [OSTI]

A Screen for Genetic Suppressor Elements of Hepatitis C Virus Identifies a Supercharged Protein Inhibitor of Viral Replication Rudo L. Simeon1, Zhilei Chen1,2* 1 Artie McFerrin Department of Chemical Engineering, Texas A&M University, College.... ACS Chemical Biology. 30. Lindenbach BD, Evans MJ, Syder AJ, Wolk B, Tellinghuisen TL, et al. (2005) Complete Replication of Hepatitis C Virus in Cell Culture. Science 309: 623 626. 31. Cowan S, Hatziioannou T, Cunningham T, Muesing MA, Gottlinger HG...

Simeon, Rudo L.; Chen, Zhilei

2013-12-31T23:59:59.000Z

249

Some physical and biological properties of the nuclear polyhedrosis virus of Heliothis zea (Boddie) and Heliothis virescens (Fabricius)  

E-Print Network [OSTI]

University Directed by: Dr. L. L. Keeley Laboratory investi ations were conducted into so ie of the physical and biological properties of the nuclear polyhedrosis virus ("!PV) of Heliothis zea (Boddie) and Peiiothi s virescens (Fabricius). information... the ranges of shapes and. sizes reported f' or other insect nuclear viruses. In contrast, the strength of alkali and dis- solving period necessary to dissolve Heliothis nuclear polyhedra are somewhat higher than those reported for other insect polyhedra...

MacFarlane, Johnny James

1969-01-01T23:59:59.000Z

250

Three dimensional colorimetric assay assemblies  

DOE Patents [OSTI]

A direct assay is described using novel three-dimensional polymeric assemblies which change from a blue to red color when exposed to an analyte, in one case a flu virus. The assemblies are typically in the form of liposomes which can be maintained in a suspension, and show great intensity in their color changes. Their method of production is also described.

Charych, Deborah (Albany, CA); Reichart, Anke (Albany, CA)

2000-01-01T23:59:59.000Z

251

The Structure of the Herpes Simplex Virus DNA-Packaging Terminase pUL15 Nuclease Domain Suggests an Evolutionary Lineage among Eukaryotic and Prokaryotic Viruses  

E-Print Network [OSTI]

, American Society for Microbiology. All Rights Reserved. doi:10.1128/JVI.00311-13 7140 jvi.asm.org Journal of Virology p. 71407148 June 2013 Volume 87 Number 12 o n June 30, 2014 by University of Kansas http://jvi.asm.org/ D ow nloaded from tron of 3... Virus Terminase June 2013 Volume 87 Number 12 jvi.asm.org 7141 o n June 30, 2014 by University of Kansas http://jvi.asm.org/ D ow nloaded from RESULTS AND DISCUSSION Overall structure of pUL15C. The pUL15C crystallizes with three molecules per...

Sigamani, Sundaresan Selvarajan; Zhao, Haiyan; Kamau, Yvonne N.; Baines, Joel D.; Tang, Liang

2013-04-17T23:59:59.000Z

252

CONSERVED VIRUS PROTEIN FAMILIES IN BACTERIOPHAGE GENOMES AND IN METAGENOMES OF HUMANS  

E-Print Network [OSTI]

people that have helped me during my graduate study at Stowers Institute for Medical Research and Kansas University Medical Center. I am especially thankful to my advisor, Dr. Arcady Mushegian for his guidance, support, advice and encouragement... of Appendices Page Appendix A: the annotation of POGs-2007..52 Appendix B: parameters for Roche 454 Newbler....100 Appendix C: the virus protein orthologs identified in Metagenomics data.102 1...

Cai, Xixu

2011-08-31T23:59:59.000Z

253

Role of the Leucine Zipper of Marek's Disease Virus Oncoprotein Meq in Pathogenesis  

E-Print Network [OSTI]

to the Ofice of Graduate Studies of Texas A&M University in partial fulfilment of the requirements for the degre of DOCTOR OF PHILOSOPHY Approved by: Chair of Commite, Sanjay Reddy Commite Members, Blanca Lupiani Elen Collison Weston Porter Yanan..., and is * Reprinted with permision from ?Homodimerization of Marek?s Disease Virus-Encoded Meq Protein Is Not Sufficient for Transformation of Lymphocytes in Chickens? by Paulete F. Suchodolski, Yoshihiro Izumiya, Blanca Lupiani, Dharani K. Ajithdos, Oren Gilad...

Suchodolski, Paulette F.

2010-07-14T23:59:59.000Z

254

First Characterization of Avian Memory T Lymphocyte Responses to Avian Influenza Virus Proteins  

E-Print Network [OSTI]

Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Approved by: Co-Chairs of Committee, Ellen W. Collisson Blanca Lupiani Committee Members, Julian...) Shailbala Singh, B.S., G.B. Pant University of Agriculture and Technology Co-Chairs of Advisory Committee: Dr. Ellen W. Collisson Dr. Blanca Lupiani Although wild birds are natural hosts of avian influenza viruses (AIVs...

Singh, Shailbala

2011-02-22T23:59:59.000Z

255

An immunofluorescent and histopathologic study of chicken embryos infected with blue-tongue virus  

E-Print Network [OSTI]

that 52 morbidity and mortality estimates are too low. The disease in susceptible young deer appears to be uniformly fatal under experi- mental conditions. Attenuated bluetongue virus (BTV) also has been 87 responsible for death and congenital... was fixed in Zenker's fluid for 6 to 8 hours, washed in running tap water for 12 to 18 hours, and stored in 702 ethanol until embed- ded in paraffin. All choriosllantoic membranes were harvested, spread on glass slides and then were examined...

Fairchild, David George

1968-01-01T23:59:59.000Z

256

Characterization of the Meq oncoproteins of Marek's disease virus vaccine strain CVI988/Rispens  

E-Print Network [OSTI]

the condition in four adult male chickens in 1907 (Biggs, 1961; Marek, 1907). He described the disease as a "polyneuritis" or a ?neuritis interstitialis?, characterized by paralysis of the legs and wings. Grossly, the sacral plexuses and spinal cords were... leukosis virus (Biggs, 1961; Campbel, 1961; Elermann, 1922). Based on susceptibility, organs afected, and histopathogenesis, the disease can be distinguished from lymphoid leucosis and a new name, Marek?s disease, was proposed (Biggs, 1961). Later...

Ajithdoss, Dharani K.

2010-07-14T23:59:59.000Z

257

Structural analysis of Herpes Simplex Virus by optical super-resolution imaging  

E-Print Network [OSTI]

, this approach requires extensive calibration protocols30 and will be the topic of future studies. We were also able to resolve and quantify the capsid spatial offset in HSV-1 particles, using precise localization of both the centre of the capsid and those... viruses, including coronaviruses (for example, the SARS, severe acute respiratory syndrome, or MERS, Middle East respiratory syndrome, coronaviruses) and rotaviruses. However, the spherical symmetry is not an a priori constraint: any system is amenable...

Laine, Romain F.; Albecka, Anna; van de Linde, Sebastian; Rees, Eric J.; Crump, Colin M.; Kaminski, Clemens F.

2015-01-22T23:59:59.000Z

258

Seroepidemiological investigation of vertebrate hosts of bluetongue virus in West Texas  

E-Print Network [OSTI]

18 20 SUMMARY ~ ~ ~ 24 REFERENCES. 25 VITA 29 LIST OF TABLES Table Page Results of agar gel precipitin tests for bluetongue antibody in sera from domestic specjes. . . . . . . . . . . . . . . . . . 16 Results of agar gel precipitin tests... for bluetongue antibody in sera from wildlife species. . . . . . . . . . . . . . . . . . 17 Comparison of bluetongue virus test results by complement fixation (CF) and agar gel precipitin (AGP) technique, on bovine and ovine sera . . ~ 19 CHAPTER I...

Snydelaar, Andrew Charles

2012-06-07T23:59:59.000Z

259

New Insights Into the Role of Equine Infectious Anemia Virus S2 Protein in Disease Expression  

E-Print Network [OSTI]

.6 Validation of TaqMan primer/probe sets and SYBR Green primers ......... 65 1 CHAPTER I AN INTRODUCTION TO EQUINE INFECTIOUS ANEMIA VIRUS, THE DISEASE AND THE S2 PROTEIN History Equine Infectious Anemia (EIA), also known as ?swamp fever.... Although other serologic and PCR based assays have been developed, the Coggins test is the international ?gold standard? procedure for routine testing of EIA (Cook et al., 2002; Issel and Cook, 1993; Nagarajan and Simard, 2001...

Covaleda Salas, Lina M.

2011-08-08T23:59:59.000Z

260

Identification of two distinct strains of watermelon mosaic virus-2 affecting cucurbits in Texas  

E-Print Network [OSTI]

maintained at 25 + 5 C in the greenhouse. Seedlings were inoculated by dusting the cotyledons with 600 mesh carborundum and rubbing them with the inoculum. Seedlings as controls were inoculated healthy sap, Host range Certain plant species exhibit... buffer and 600 mesh carborundum and then rubbed on squash cotyledons. The same procedure was repeated w1th healthy pollen. b. Pollen from 1nfected plants was examined for virus presence by serolog1cal test. Pollen was prepared as follows: 0. 2 g...

Chala, Victor Hugo

1983-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Acute Hendra virus infection: Analysis of the pathogenesis and passive antibody protection in the hamster model  

SciTech Connect (OSTI)

Hendra virus (HeV) and Nipah virus (NiV) are recently-emerged, closely related and highly pathogenic paramyxoviruses. We have analysed here the pathogenesis of the acute HeV infection using the new animal model, golden hamster (Mesocricetus auratus), which is highly susceptible to HeV infection. HeV-specific RNA and viral antigens were found in multiple organs and virus was isolated from different tissues. Dual pathogenic mechanism was observed: parenchymal infection in various organs, including the brain, with vasculitis and multinucleated syncytia in many blood vessels. Furthermore, monoclonal antibodies specific for the NiV fusion protein neutralized HeV in vitro and efficiently protected hamsters from HeV if given before infection. These results reveal the similarities between HeV and NiV pathogenesis, particularly in affecting both respiratory and neuronal system. They demonstrate that hamster presents a convenient novel animal model to study HeV infection, opening new perspectives to evaluate vaccine and therapeutic approaches against this emergent infectious disease.

Guillaume, Vanessa [Inserm U758, Human Virology, F-69365 (France); Ecole Normale Superieure de Lyon, Lyon, F-69007 (France); IFR128 BioSciences Lyon-Gerland Lyon-Sud, University of Lyon 1, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07 (France); Wong, K. Thong; Looi, R.Y. [Department of Pathology, University of Malaya, Kuala Lumpur (Malaysia); Georges-Courbot, Marie-Claude; Barrot, Laura [Laboratory P4-Jean Merieux, INSERM, Lyon (France); Buckland, Robin; Wild, T. Fabian [Inserm U758, Human Virology, F-69365 (France); Ecole Normale Superieure de Lyon, Lyon, F-69007 (France); IFR128 BioSciences Lyon-Gerland Lyon-Sud, University of Lyon 1, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07 (France); Horvat, Branka, E-mail: branka.horvat@inserm.f [Inserm U758, Human Virology, F-69365 (France); Ecole Normale Superieure de Lyon, Lyon, F-69007 (France); IFR128 BioSciences Lyon-Gerland Lyon-Sud, University of Lyon 1, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07 (France)

2009-05-10T23:59:59.000Z

262

Mutations Abrogating VP35 Interaction with Double-Stranded RNA Render Ebola Virus Avirulent in Guinea Pigs  

SciTech Connect (OSTI)

Ebola virus (EBOV) protein VP35 is a double-stranded RNA (dsRNA) binding inhibitor of host interferon (IFN)-{alpha}/{beta} responses that also functions as a viral polymerase cofactor. Recent structural studies identified key features, including a central basic patch, required for VP35 dsRNA binding activity. To address the functional significance of these VP35 structural features for EBOV replication and pathogenesis, two point mutations, K319A/R322A, that abrogate VP35 dsRNA binding activity and severely impair its suppression of IFN-{alpha}/{beta} production were identified. Solution nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography reveal minimal structural perturbations in the K319A/R322A VP35 double mutant and suggest that loss of basic charge leads to altered function. Recombinant EBOVs encoding the mutant VP35 exhibit, relative to wild-type VP35 viruses, minimal growth attenuation in IFN-defective Vero cells but severe impairment in IFN-competent cells. In guinea pigs, the VP35 mutant virus revealed a complete loss of virulence. Strikingly, the VP35 mutant virus effectively immunized animals against subsequent wild-type EBOV challenge. These in vivo studies, using recombinant EBOV viruses, combined with the accompanying biochemical and structural analyses directly correlate VP35 dsRNA binding and IFN inhibition functions with viral pathogenesis. Moreover, these studies provide a framework for the development of antivirals targeting this critical EBOV virulence factor.

Prins, Kathleen C.; Delpeut, Sebastien; Leung, Daisy W.; Reynard, Olivier; Volchkova, Valentina A.; Reid, St. Patrick; Ramanan, Parameshwaran; Crdenas, Washington B.; Amarasinghe, Gaya K.; Volchkov, Viktor E.; Basler, Christopher F. (CNRS-INSERM); (Mount Sinai Hospital); (LB-Ecuador); (Iowa State)

2010-10-11T23:59:59.000Z

263

Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain of the heliozoon Acanthocystis turfacea  

E-Print Network [OSTI]

Sequence and annotation of the 288-kb ATCV-1 virus that infects an endosymbiotic chlorella strain 2007 Abstract Acanthocystis turfacea chlorella virus (ATCV-1), a prospective member of the family Phycodnaviridae, genus Chlorovirus, infects a unicellular, eukaryotic, chlorella-like green alga, Chlorella SAG 3

Graves, Michael V.

264

The impact of social stress on acute Theiler's murine encephalitis virus infection.  

E-Print Network [OSTI]

of the same patience and brilliance. My undergraduate helpers were essential and my gratitude immeasurable; the list is long, but a few stood out: Larissa Husband, Gabby Oroza, Heath McCullough, and Patrick Bridegam- you guys are the best! Heath... the divergent effects of stress on disease course. Theiler?s virus infection in mice has been used for many decades to study MS. Using this model, the many possible aspects of stress- immune interactions can be explored in a controlled manner. Theiler...

Johnson, Robin Ranee

2004-09-30T23:59:59.000Z

265

Host genetics of Epstein-Barr virus infection, latency and disease  

E-Print Network [OSTI]

. Dean M, Carrington M, Winkler C, et al. Genetic restriction of HIV-1 infection and progression to 511 AIDS by a deletion allele of the CKR5 structural gene. Hemophilia Growth and Development Study, 512 Multicenter AIDS Cohort Study, Multicenter... Therapy 2014; 25: A9-A9. 531 19. Cohen JI, Mocarski ES, Raab-Traub N, Corey L, Nabel GJ. The need and challenges for 532 development of an Epstein-Barr virus vaccine. Vaccine 2013; 31 Suppl 2: B194-196. DOI: S0264-533 410X(12)01361-8 [pii] 534 10...

Houldcroft, Charlotte J.; Kellam, Paul

2014-01-01T23:59:59.000Z

266

Surface localization of the nuclear receptor CAR in influenza A virus-infected cells  

SciTech Connect (OSTI)

Constitutive active/androstane receptor CAR is a member of the nuclear receptors which regulate transcription of xenobiotic metabolism enzymes. CAR is usually localized in the cytosol and nucleus. Here, we found that CAR was localized at the cell surface of influenza A virus (IAV)-infected cells. Additionally, we demonstrated that expression of a viral envelope glycoprotein, either hemagglutinin (HA) or neuraminidase (NA), but not viral nucleoprotein (NP), was responsible for this localization. This report is the first demonstration of CAR at the surface of tissue culture cells, and suggests that CAR may exert the IAV infection mechanism.

Takahashi, Tadanobu [Department of Pharmaco-Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and COE Program in the 21st Century, 52-1 Yada, Shizuoka 422-8526 (Japan); Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, CREST, JST, and COE Program in the 21st Century, Shizuoka 422-8526 (Japan); Moriyama, Yusuke; Ikari, Akira; Sugatani, Junko [Department of Pharmaco-Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and COE Program in the 21st Century, 52-1 Yada, Shizuoka 422-8526 (Japan); Suzuki, Takashi [Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, CREST, JST, and COE Program in the 21st Century, Shizuoka 422-8526 (Japan); Miwa, Masao [Department of Pharmaco-Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and COE Program in the 21st Century, 52-1 Yada, Shizuoka 422-8526 (Japan)], E-mail: miwa@u-shizuoka-ken.ac.jp

2008-04-11T23:59:59.000Z

267

The relationship of arthropods to the transmission of the virus of cotton mosaic  

E-Print Network [OSTI]

THE RELATIONSHIP QF ARTHROPCGS TO THE TRANSMISSION OF THE VIRUS OF COTTON MOSAIG A Thesis By MIGHAEL FRANK SGHUSTER Submitted to the Graduate School of the Agxicultural and Mechanical College of Texas in partial fulfillment of the requirements... for the degree of MASTER OF SCIENCE Major Sub)act t g g I A 0 Z 0 p U3 F THE RELATIONSHIP OF ARTHROPODS TO THE TRANSMISSION OF THE VIRVS OF COTTON MOSAIC A Theais By MICHAEL. FRANK SGHUSTER Appzoved aa toatyle and content by: Gha raa o C ittee...

Schuster, Michael Frank

2012-06-07T23:59:59.000Z

268

Influenza A/H7N9 virus emergence in poultry and humans in Guangdong China  

E-Print Network [OSTI]

Peer Review Influenza A/H7N9 virus emergence in poultry and humans in Guangdong China Changwen Ke 1,Jing Lu 1, 2, Jie Wu 1, Dawei Guan 1, Lirong Zou 1,Tie Song1, Lina Yi 1, 2, Xianqiao Zeng 1, 2 , Lijun Liang 1, Hanzhong Ni 1, Min Kang 1, Xin... Running Title: H7N9 emergence in poultry and humans Corresponding authors: Marion Koopmans Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands Center for Infectious Diseases, National Institute of Public Health and the Environment...

Ke, Changwen; Lu, Jing; Wu, Jie; Guan, Dawei; Zou, Lirong; Song, Tie; Yi, Lina; Zeng, Xianqiao; Liang, Lijun; Ni, Hanzhong; Kang, Min; Zhang, Xin; Zhong, Haojie; He, Jianfeng; Lin, Jinyan; Smith, Derek; Burke, David; Fouchier, Ron A. M.; Koopmans, Marion; Zhang, Yonghui

2014-08-27T23:59:59.000Z

269

Reaction of single-cross hybrid corn to sorghum yellow banding virus  

E-Print Network [OSTI]

leaving the large solid materials behind. Carborundum (Silicon carbide), 600 mesh, was added to 1 percent (weight/volume), or 10 gram per liter. The SYBV suspension was kept at a cold temperature to maintain infectivity of the virus, and it was used..., from a distance of 2-5 cm, with an inoculum flow rate of 20 ml/minute, approximately. 10 Sprayers were shaken frequently during inoculation to keep carborundum in suspension. The following characters were studied in the experiment. ant hei h...

Pe, Aye

1990-01-01T23:59:59.000Z

270

Comparative promoter activities of three endogenous copies of mouse mammary tumor virus  

E-Print Network [OSTI]

to 12 CC ~ o c 0 tu x MMTV M13 pLC1 ?(, a~ CAT pBR322 0/ I SV40 Figure 5. Diagram of pLC1. The plasmid pLC1 (Toohey et al. , 1986) contains an Intact LTR obtained from the C3H provirus as a Pst I restriction fragment. The plasmid also...COMPARATIVE PROMOTER ACTIVITIES OF THREE ENDOGENOUS COPIES OF MOUSE MAMMARY TUMOR VIRUS A Thesis by LAURA REID VILANDER Submitted to the Graduate College of Texas A&M University in partial fulfilment of the requirements for the degree...

Vilander, Laura Reid

1987-01-01T23:59:59.000Z

271

A cell based high-throughput screening approach for the discovery of new inhibitors of respiratory syncytial virus  

E-Print Network [OSTI]

-based higsyncytial virus Dong-Hoon Chung1,2, Blake P Moore3,5, Daljit S Matharu4, Jennifer E Golden4, Clinton Maddox3, Lynn Rasmussen3, Melinda I Sosa3, Subramaniam Ananthan3, E Lucile White3, Fuli Jia3,6, Colleen B Jonsson1,2 and William E Severson1* Abstract... syncytial virus. Handbooks in Health Care Co. In Edited by Weisman LE, Groothuis JR. Newtown: PA; 2000:723. 18. Hall CB, et al: Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. a randomized double-blind study. N Engl J...

Chung, Dong-Hoon; Moore, Blake P.; Matharu, Daljit S.; Golden, Jennifer E.; Maddox, Clinton; Rasmussen, Lynn; Sosa, Melinda I.; Ananthan, Subramaniam; White, E. Lucile; Jia, Fuli; Jonsson, Colleen B.; Severson, William E.

2013-01-10T23:59:59.000Z

272

Electron microscopic investigations and indexing studies of psorosis and citrus ringspot virus of citrus / by Margaret Atchison Barkley  

E-Print Network [OSTI]

-253. In W. C. Price, (ed. ) Pro- ceedings 3rd Conf. Intern. Organ. Citrus Virol. Univ. of Florida Press, Gainesville. 1968. Purification of citrus crinkly-leaf virus. p. 255-263. In J. F. L Childs, (ed. ) Proceedings 4th Conf. Intern. Organ. Citrus...:519-520. 19. 1961. Mechanical transmission of infectious variega- tion virus in citrus and non-citrus hosts. p. 197-204. In W. C. Price, (ed. ) Proc. 2nd Conf. Intern. Organ. Citrus Virol. Univ. of Florida Press, Gainesville. 20. Greenwood, A. P, , R. M...

Barkley, Margaret Atchison

1979-01-01T23:59:59.000Z

273

Crystal Structure of the Conserved Herpes Virus Fusion Regulator Complex gHgL  

SciTech Connect (OSTI)

Herpesviruses, which cause many incurable diseases, infect cells by fusing viral and cellular membranes. Whereas most other enveloped viruses use a single viral catalyst called a fusogen, herpesviruses, inexplicably, require two conserved fusion-machinery components, gB and the heterodimer gH-gL, plus other nonconserved components. gB is a class III viral fusogen, but unlike other members of its class, it does not function alone. We determined the crystal structure of the gH ectodomain bound to gL from herpes simplex virus 2. gH-gL is an unusually tight complex with a unique architecture that, unexpectedly, does not resemble any known viral fusogen. Instead, we propose that gH-gL activates gB for fusion, possibly through direct binding. Formation of a gB-gH-gL complex is critical for fusion and is inhibited by a neutralizing antibody, making the gB-gH-gL interface a promising antiviral target.

Chowdary, T.; Cairns, T; Atanasiu, D; Cohen, G; Eisenberg, R; Heldwein, E

2010-01-01T23:59:59.000Z

274

Borna disease virus nucleoprotein inhibits type I interferon induction through the interferon regulatory factor 7 pathway  

SciTech Connect (OSTI)

Highlights: IRF7 nuclear localisation was inhibited by BDV persistently infected. BDV N protein resistant to IFN induction both in BDV infected OL cell and N protein plasmid transfected OL cell. BDV N protein is related to the inhibition of IRF7 nuclear localisation. -- Abstract: The expression of type I interferon (IFN) is one of the most potent innate defences against viral infection in higher vertebrates. Borna disease virus (BDV) establishes persistent, noncytolytic infections in animals and in cultured cells. Early studies have shown that the BDV phosphoprotein can inhibit the activation of type I IFN through the TBK1IRF3 pathway. The function of the BDV nucleoprotein in the inhibition of IFN activity is not yet clear. In this study, we demonstrated IRF7 activation and increased IFN-?/? expression in a BDV-persistently infected human oligodendroglia cell line following RNA interference-mediated BDV nucleoprotein silencing. Furthermore, we showed that BDV nucleoprotein prevented the nuclear localisation of IRF7 and inhibited endogenous IFN induction by poly(I:C), coxsackie virus B3 and IFN-?. Our findings provide evidence for a previously undescribed mechanism by which the BDV nucleoprotein inhibits type I IFN expression by interfering with the IRF7 pathway.

Song, Wuqi [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China) [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China); Department of Microbiology, Harbin Medical University (China); Kao, Wenping [The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions (China) [The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions (China); Department of Microbiology, Harbin Medical University (China); Zhai, Aixia [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China)] [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China); Qian, Jun; Li, Yujun [The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions (China)] [The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions (China); Zhang, Qingmeng [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China)] [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China); Zhao, Hong; Hu, Yunlong; Li, Hui [Department of Microbiology, Harbin Medical University (China)] [Department of Microbiology, Harbin Medical University (China); Zhang, Fengmin, E-mail: fengminzhang@ems.hrbmu.edu.cn [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China) [The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China); The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions (China); Department of Microbiology, Harbin Medical University (China)

2013-09-06T23:59:59.000Z

275

Complex adenovirus-vectored vaccine protects guinea pigs from three strains of Marburg virus challenges  

SciTech Connect (OSTI)

The Marburg virus (MARV), an African filovirus closely related to the Ebola virus, causes a deadly hemorrhagic fever in humans, with up to 90% mortality. Currently, treatment of disease is only supportive, and no vaccines are available to prevent spread of MARV infections. In order to address this need, we have developed and characterized a novel recombinant vaccine that utilizes a single complex adenovirus-vectored vaccine (cAdVax) to overexpress a MARV glycoprotein (GP) fusion protein derived from the Musoke and Ci67 strains of MARV. Vaccination with the cAdVaxM(fus) vaccine led to efficient production of MARV-specific antibodies in both mice and guinea pigs. Significantly, guinea pigs vaccinated with at least 5 x 10{sup 7} pfu of cAdVaxM(fus) vaccine were 100% protected against lethal challenges by the Musoke, Ci67 and Ravn strains of MARV, making it a vaccine with trivalent protective efficacy. Therefore, the cAdVaxM(fus) vaccine serves as a promising vaccine candidate to prevent and contain multi-strain infections by MARV.

Wang Danher [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Hevey, Michael [Virology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD 21702-5011 (United States); Juompan, Laure Y. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Trubey, Charles M. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Raja, Nicholas U. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Deitz, Stephen B. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Woraratanadharm, Jan [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Luo Min [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Yu Hong [Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave., BSB 201, Charleston, SC 29403 (United States); Swain, Benjamin M. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Moore, Kevin M. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States); Dong, John Y. [Division of Bio-defense Vaccines, GenPhar Inc., 871 Lowcountry Blvd., Mount Pleasant, SC 29464 (United States) and Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave., BSB 201, Charleston, SC 29403 (United States)]. E-mail: dongj@genphar.com

2006-09-30T23:59:59.000Z

276

Self-Assembly of Virus-Structured High Surface Area Nanomaterials and Their Application as Battery Electrodes  

E-Print Network [OSTI]

. In Final Form: October 12, 2007 High area nickel and cobalt surfaces were assembled using modified Tobacco-fold increase in surface area. Electroless deposition of ionic metals onto surface-assembled virus templates produced uniform metal coatings up to 40 nm in thickness. Within a nickel-zinc battery system

Rubloff, Gary W.

277

Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi  

E-Print Network [OSTI]

Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483

Graves, Michael V.

278

Structural transitions and energy landscape for Cowpea Chlorotic Mottle Virus capsid mechanics from nanomanipulation in vitro and in silico  

E-Print Network [OSTI]

Physical properties of capsids of plant and animal viruses are important factors in capsid self-assembly, survival of viruses in the extracellular environment, and their cell infectivity. Virus shells can have applications as nanocontainers and delivery vehicles in biotechnology and medicine. Combined AFM experiments and computational modeling on sub-second timescales of the indentation nanomechanics of Cowpea Chlorotic Mottle Virus (CCMV) capsid show that the capsid's physical properties are dynamic and local characteristics of the structure, which depend on the magnitude and geometry of mechanical input. Surprisingly, under large deformations the CCMV capsid transitions to the collapsed state without substantial local structural alterations. The enthalpy change in this deformation state dH = 11.5 - 12.8 MJ/mol is mostly due to large-amplitude out-of-plane excitations, which contribute to the capsid bending, and the entropy change TdS = 5.1 - 5.8 MJ/mol is mostly due to coherent in-plane rearrangements of pr...

Kononova, Olga; Brasch, Melanie; Cornelissen, Jeroen; Dima, Ruxandra I; Marx, Kenneth A; Wuite, Gijs J L; Roos, Wouter H; Barsegov, Valeri

2015-01-01T23:59:59.000Z

279

Localized or Systemic {italic In Vivo} Heat-Inactivation of Human Immunodeficiency Virus (HIV): A Mathematical Analysis  

SciTech Connect (OSTI)

Temperatures as low as 42 C, maintained for a little as 25 minutes, inactivate {approx}25% of HIV. Furthermore, human immunodeficiency virus (HIV)-infected T-cells are more sensitive to heat than healthy lymphocytes and susceptibility increases when the cells are pre-sensitized by exposure to tumor necrosis factor. Thus, induction of a whole-body hyperthermia, or hyperthermia specifically limited to tissues having a high viral load, are potential antiviral therapies for acquired immunodeficiency disease (AIDS). Accordingly, we incorporated therapeutic hyperthermia into an existing mathematical model which evaluates the interaction between HIV and CD4{sup +} T cells. Given the assumptions and limitations of this model, the results indicate that a daily therapy, reducing the population of actively infected cells by 40% or infectious virus by 50%, would effectively reverse the depletion of T cells. In contrast, a daily reduction of 20% of either actively infected cells or infectious virus would have a marginal effect. However, reduction by 20% of both actively infected cells and infectious virus could restore T cell numbers, assuming that permanent damage had not been inflicted on the thymus. Whole-body hyperthermia seems unlikely to be clinically useful, unless it can be induced non-invasively without general anesthesia. In contrast, heating directed specifically to areas of viral concentration may be effective and have a suitable risk/benefit ratio.

Pennypacker, Carl R.; Perelson, A.S.; Nys, N.; Nelson, G.; Sessler, D.I.

1993-12-15T23:59:59.000Z

280

Solution structure and functional analysis of a frameshift-stimulating RNA pseudoknot from sugarcane yellow leaf virus  

E-Print Network [OSTI]

Plant luteoviral RNA viruses employ -1 frameshifting for the production of P1 and P1-P2 fusion proteins important for viral replication. Luteoviral pseudoknots are characterized by three adenosines in the 3' side of loop L2 known to be important...

Cornish, Peter Verle

2006-04-12T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

J Vector Borne Dis 47, December 2010, pp. 211216 Molecular epidemiology of Crimean-Congo hemorrhagic fever virus  

E-Print Network [OSTI]

for reducing population density of potential tick vectors in this province. Further surveys are indicated and distribution of CCHFV in the tick population of Hamadan province of Iran. Method: In this study, RT-PCR has in hospi- tals16­17. The virus is distributed across Africa, Eastern Europe, the Middle East, and Asia

Paris-Sud XI, Université de

282

A SPATIAL SIRS BOOLEAN NETWORK MODEL FOR THE SPREAD OF H5N1 AVIAN INFLUENZA VIRUS AMONG POULTRY FARMS  

E-Print Network [OSTI]

A SPATIAL SIRS BOOLEAN NETWORK MODEL FOR THE SPREAD OF H5N1 AVIAN INFLUENZA VIRUS AMONG POULTRY Influenza and Poultry Disease Epidemiology, Federal Centre for Animal Health, 600901 Yur'evets, Vladimir-Infected-Recovered-Susceptible (SIRS) Boolean network of poultry farms, using proba- bilistic Boolean rules. Gravity models from

Matache, Dora

283

Development and characterization of a Rift Valley fever virus cell-cell fusion assay using alphavirus replicon vectors  

SciTech Connect (OSTI)

Rift Valley fever virus (RVFV), a member of the Phlebovirus genus in the Bunyaviridae family, is transmitted by mosquitoes and infects both humans and domestic animals, particularly cattle and sheep. Since primary RVFV strains must be handled in BSL-3+ or BSL-4 facilities, a RVFV cell-cell fusion assay will facilitate the investigation of RVFV glycoprotein function under BSL-2 conditions. As for other members of the Bunyaviridae family, RVFV glycoproteins are targeted to the Golgi, where the virus buds, and are not efficiently delivered to the cell surface. However, overexpression of RVFV glycoproteins using an alphavirus replicon vector resulted in the expression of the glycoproteins on the surface of multiple cell types. Brief treatment of RVFV glycoprotein expressing cells with mildly acidic media (pH 6.2 and below) resulted in rapid and efficient syncytia formation, which we quantified by {beta}-galactosidase {alpha}-complementation. Fusion was observed with several cell types, suggesting that the receptor(s) for RVFV is widely expressed or that this acid-dependent virus does not require a specific receptor to mediate cell-cell fusion. Fusion occurred over a broad temperature range, as expected for a virus with both mosquito and mammalian hosts. In contrast to cell fusion mediated by the VSV-G glycoprotein, RVFV glycoprotein-dependent cell fusion could be prevented by treating target cells with trypsin, indicating that one or more proteins (or protein-associated carbohydrate) on the host cell surface are needed to support membrane fusion. The cell-cell fusion assay reported here will make it possible to study the membrane fusion activity of RVFV glycoproteins in a high-throughput format and to screen small molecule inhibitors for the ability to block virus-specific membrane fusion.

Filone, Claire Marie [Department of Microbiology, University of Pennsylvania, 301 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 (United States); Heise, Mark [Departments of Genetics and Microbiology and Immunology, The Carolina Vaccine Institute, University of North Carolina, Chapel Hill, NC 27599 (United States); Doms, Robert W. [Department of Microbiology, University of Pennsylvania, 301 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 (United States)]. E-mail: doms@mail.med.upenn.edu; Bertolotti-Ciarlet, Andrea [Department of Microbiology, University of Pennsylvania, 301 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104 (United States)]. E-mail: aciarlet@mail.med.upenn.edu

2006-12-20T23:59:59.000Z

284

Evolving T-cell vaccine strategies for HIV, the virus with a thousand faces  

SciTech Connect (OSTI)

HIV's rapid global spread and the human suffering it has left in its wake have made AIDS a global heath priority for the 25 years since its discovery. Yet its capacity to rapidly evolve has made combating this virus a tremendous challenge. The obstacles to creating an effective HIV vaccine are formidable, but there are advances in the field on many fronts, in terms of novel vectors, adjuvants, and antigen design strategies. SIV live attenuated vaccine models are able to confer protection against heterologous challenge, and this continues to provide opportunities to explore the biological underpinnings of a protective effect (9). More indirect, but equally important, is new understanding regarding the biology of acute infection (43), the role of immune response in long-term non-progression (6,62, 81), and defining characteristics of broadly neutralizing antibodies (4). In this review we will focus on summarizing strategies directed towards a single issue, that of contending with HIV variation in terms of designing aT-cell vaccine. The strategies that prove most effective in this area can ultimately be combined with the best strategies under development in other areas, with the hope of ultimately converging on a viable vaccine candidate. Only two large HIV vaccine efficacy trials have been completed and both have failed to prevent infection or confer a benefit to infected individual (23,34), but there is ample reason to continue our efforts. A historic breakthrough came in 1996, when it was realized that although the virus could escape from a single antiretroviral (ARV) therapy, it could be thwarted by a combination of medications that simultaneously targeted different parts of the virus (HAART) (38). This revelation came after 15 years of research, thought, and clinical testing; to enable that vital progress the research and clinical communities had to first define and understand, then develop a strategy to counter, the remarkable evolutionary potential of the virus. HAART, for the first time, provided an effective treatment to help those with living with HIV stay healthy. Nonetheless, the treatment has limitations. People with HIV face a lifetime of expensive daily multi-drug regimens, often with side effects; drug resistance at the individual and population level are issues (56); and universal access, despite substantial progress, is a dream not yet realized for many of the millions of the world's poor who are living with HIV (68). These issues, combined with the growing numbers of people infected globally and impact of HIV on society, make the development of an HIV vaccine or a prophylactic prevention strategy a crucial if elusive goal. In some ways, the history of HIV vaccine deVelopment has paralleled the early stages of designing effective therapy. We had to test the simple strategies first, but meanwhile the story of the impact of diversity from an immunological perspective is still unfolding, and novel ideas countermeasures are being explored.

Korber, Bette [Los Alamos National Laboratory

2009-01-01T23:59:59.000Z

285

The effect of dimethyl sulfoxide on tobacco tissue and tobacco mosaic virus multiplication  

E-Print Network [OSTI]

?mb i of virus lesions i&roducod by l0 half leaf r pli! Siiors with treatments applied dirr ct Ly to & elean NN! 7 eaves. 32 Number cf de i's post i"1ocuLat'ion w71en samp7es from 7eaves oi' Sar sun plants pret" sated with DNSO first produced local... with in ;: f'e, days after the inif iation of the experj ment. cssav of tis, u ~ f?oa: this shoot . :as made on the iw. clith day post inoc:la' ior an ! the viru- was detected. The same plant beg:n to fiowe. o? Ll, fo '. teenth day of tl"e exp riment. ). 11...

Miller, Lester Lynn

1969-01-01T23:59:59.000Z

286

Ultrastructural study of Rift Valley fever virus in the mouse model  

SciTech Connect (OSTI)

Detailed ultrastructural studies of Rift Valley fever virus (RVFV) in the mouse model are needed to develop and characterize a small animal model of RVF for the evaluation of potential vaccines and therapeutics. In this study, the ultrastructural features of RVFV infection in the mouse model were analyzed. The main changes in the liver included the presence of viral particles in hepatocytes and hepatic stem cells accompanied by hepatocyte apoptosis. However, viral particles were observed rarely in the liver; in contrast, particles were extremely abundant in the CNS. Despite extensive lymphocytolysis, direct evidence of viral replication was not observed in the lymphoid tissue. These results correlate with the acute-onset hepatitis and delayed-onset encephalitis that are dominant features of severe human RVF, but suggest that host immune-mediated mechanisms contribute significantly to pathology. The results of this study expand our knowledge of RVFV-host interactions and further characterize the mouse model of RVF.

Reed, Christopher; Steele, Keith E.; Honko, Anna; Shamblin, Joshua; Hensley, Lisa E. [United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States)] [United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States); Smith, Darci R., E-mail: darci.smith1@us.army.mil [United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States)

2012-09-15T23:59:59.000Z

287

Phylogenomic analysis of 11 complete African swine fever virus genome sequences  

SciTech Connect (OSTI)

Viral molecular epidemiology has traditionally analyzed variation in single genes. Whole genome phylogenetic analysis of 123 concatenated genes from 11 ASFV genomes, including E75, a newly sequenced virulent isolate from Spain, identified two clusters. One contained South African isolates from ticks and warthog, suggesting derivation from a sylvatic transmission cycle. The second contained isolates from West Africa and the Iberian Peninsula. Two isolates, from Kenya and Malawi, were outliers. Of the nine genomes within the clusters, seven were within p72 genotype 1. The 11 genomes sequenced comprised only 5 of the 22 p72 genotypes. Comparison of synonymous and non-synonymous mutations at the genome level identified 20 genes subject to selection pressure for diversification. A novel gene of the E75 virus evolved by the fusion of two genes within the 360 multicopy family. Comparative genomics reveals high diversity within a limited sample of the ASFV viral gene pool.

Villiers, Etienne P. de, E-mail: e.villiers@cgiar.or [International Livestock Research Institute, PO Box 30709, Nairobi 00100 (Kenya); Gallardo, Carmina; Arias, Marisa [EU reference Laboratory for ASF, CISA-INIA, Crta Algete el Cesar s/n 28130 Valdeolmos, Madrid (Spain); Silva, Melissa da; Upton, Chris [Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada V8W 3P6 (Canada); Martin, Raquel [EU reference Laboratory for ASF, CISA-INIA, Crta Algete el Cesar s/n 28130 Valdeolmos, Madrid (Spain); Bishop, Richard P. [International Livestock Research Institute, PO Box 30709, Nairobi 00100 (Kenya)

2010-04-25T23:59:59.000Z

288

Analyses of the spleen proteome of chickens infected with Marek's disease virus  

SciTech Connect (OSTI)

Marek's disease virus (MDV), which causes a lymphoproliferative disease in chickens, is known to induce host responses leading to protection against disease in a manner dependent on genetic background of chickens and virulence of the virus. In the present study, changes in the spleen proteome at 7, 14 and 21 days post-infection in response to MDV infection were studied using two-dimensional polyacrylamide gel electrophoresis. Differentially expressed proteins were identified using one-dimensional liquid chromatography electrospray ionization tandem mass spectrometry (1D LC ESI MS/MS). Comparative analysis of multiple gels revealed that the majority of changes had occurred at early stages of the disease. In total, 61 protein spots representing 48 host proteins were detected as either quantitatively (false discovery rate (FDR) <= 0.05 and fold change >= 2) or qualitatively differentially expressed at least once during different sampling points. Overall, the proteins identified in the present study are involved in a variety of cellular processes such as the antigen processing and presentation, ubiquitin-proteasome protein degradation (UPP), formation of the cytoskeleton, cellular metabolism, signal transduction and regulation of translation. Notably, early stages of the disease were characterized by changes in the UPP, and antigen presentation. Furthermore, changes indicative of active cell proliferation as well as apoptosis together with significant changes in cytoskeletal components that were observed throughout the experimental period suggested the complexity of the pathogenesis. The present findings provide a basis for further studies aimed at elucidation of the role of these proteins in MDV interactions with its host.

Thanthrige-Don, Niroshan; Abdul-Careem, Mohamed F. [Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Shack, L. Allen; Burgess, Shane C. [Department of Basic Sciences, Mississippi State University (United States); Sharif, Shayan, E-mail: shayan@uoguelph.c [Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada)

2009-08-01T23:59:59.000Z

289

Complete genome sequencing and evolutionary analysis of Indian isolates of Dengue virus type 2  

SciTech Connect (OSTI)

Highlights: Complete genome of Indian DENV-2 was deciphered for the first time in this study. The recent Indian DENV-2 revealed presence of many unique amino acid residues. Genotype shift (American to Cosmopolitan) characterizes evolution of DENV-2 in India. Circulation of a unique clade of DENV-2 in South Asia was identified. -- Abstract: Dengue is the most important arboviral infection of global public health significance. It is now endemic in most parts of the South East Asia including India. Though Dengue virus type 2 (DENV-2) is predominantly associated with major outbreaks in India, complete genome information of Indian DENV-2 is not available. In this study, the full-length genome of five DENV-2 isolates (four from 2001 to 2011 and one from 1960), from different parts of India was determined. The complete genome of the Indian DENV-2 was found to be 10,670 bases long with an open reading frame coding for 3391 amino acids. The recent Indian DENV-2 (20012011) revealed a nucleotide sequence identity of around 90% and 97% with an older Indian DENV-2 (1960) and closely related Sri Lankan and Chinese DENV-2 respectively. Presence of unique amino acid residues and non-conservative substitutions in critical amino acid residues of major structural and non-structural proteins was observed in recent Indian DENV-2. Selection pressure analysis revealed positive selection in few amino acid sites of the genes encoding for structural and non-structural proteins. The molecular phylogenetic analysis based on comparison of both complete coding region and envelope protein gene with globally diverse DENV-2 viruses classified the recent Indian isolates into a unique South Asian clade within Cosmopolitan genotype. A shift of genotype from American to Cosmopolitan in 1970s characterized the evolution of DENV-2 in India. Present study is the first report on complete genome characterization of emerging DENV-2 isolates from India and highlights the circulation of a unique clade in South Asia.

Dash, Paban Kumar, E-mail: pabandash@rediffmail.com; Sharma, Shashi; Soni, Manisha; Agarwal, Ankita; Parida, Manmohan; Rao, P.V.Lakshmana

2013-07-05T23:59:59.000Z

290

Versatile Three-Dimensional Virus-Based Template for Dye-Sensitized Solar Cells with Improved Electron Transport and Light Harvesting  

E-Print Network [OSTI]

By genetically encoding affinity for inorganic materials into the capsid proteins of the M13 bacteriophage, the virus can act as a template for the synthesis of nanomaterial composites for use in various device applications. ...

Chen, Po-Yen

291

Common Cold Self Care The "common cold" is inflammation of the upper respiratory tract caused a variety of different viruses. Antibiotics do not  

E-Print Network [OSTI]

for 3 to 4 weeks. Cold viruses are spread from person to person through coughs, sneezes, and mucus symptoms, facial pressure, and cough are no better or worse after 1 ­ 2 weeks, consider contacting

292

Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications  

E-Print Network [OSTI]

The zebrafish has various advantages as a model organism to analyze the structure and function of neural circuits but efficient viruses or other tools for fast gene transfer are lacking. We show that transgenes can be ...

Zou, Ming

293

An assay of duck hepatitis virus induced interferon, produced in duck embryo fibro-blasts which have experienced short term treatment with DDT  

E-Print Network [OSTI]

AN ASSAY OP DUCK HEPATITIS VIRUS INDUCED INTERFERON, PRODUCED IN DUCK EMBRYO FIBROBLASTS WHICH HAVE EXPERIENCED SHORT TERM TREATMENT WITH DDT A Thesis by BURGESS BAUDER Submitted to the Graduate College of Texas A&M University in Partial... WITH DDT A Thesi. s by /'. ". " . "i BURGESS BAUDER Approved as to style and content by: (Chairm of Committee) (Head of Depar ent) (Member) (Member) (Member) August 1973 ABSTRACT An Assay of Duck Hepatitis Virus Induced Interferon, Produced...

Bauder, Richard Burgess

1973-01-01T23:59:59.000Z

294

Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A  

E-Print Network [OSTI]

Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A

Graves, Michael V.

295

A study of the interaction between humoral canine distemper antibody and canine distemper virus-infected cells (monolayers of canine brain  

E-Print Network [OSTI]

A STUDY OF THE INTERACTION BETWEEN HUMORAL CANINE DISTEMPER ANTIBODY AND CANINE DISTEMPER VIRUS INFECTED CELLS (MONOLAYERS OF CANINE BRAIN) A Thesis by WILLIAM DEAN JOHNSON Submitted to the Graduate College of Texas A&X University in partial... fulfillment of the requirement for the degree of MASTER OF SCIENCE May I973 Major Subject: Veterinary Pathology A STUDY OF THE INTERACTION BETWEEN HUMORAL CANINE DISTEMPER ANTIBODY AND CANINE DISTEMPER VIRUS INFECTED CELLS (MONOLAYERS OF CANINE BRAIN) A...

Johnson, William Dean

2012-06-07T23:59:59.000Z

296

Antigenic Variation of Clade 2.1 H5N1 Virus Is Determined by a Few Amino Acid Substitutions Immediately Adjacent to the Receptor Binding Site  

E-Print Network [OSTI]

Infectious Diseases, University of Cambridge, Cambridge, United Kingdomc; JAPFA Comfeed, Indonesiad; Department of Veterinary Medicine, University of Maryland, College Park, and Virginia-Maryland Regional College of Veterinary Medicine, College Park, Maryland... as 2005. After a series of HPAI H5N1 virus outbreaks from 2006 to 2008 and as a result of monitoring of avian influenza virus variants by the Indo- nesian OFFLU project (9), the poultry vaccine recommendation was updated to contain an A/Chicken/West Java...

Koel, Bjrn F.; van der Vliet, Stefan; Burke, David F.; Bestebroer, Theo M.; Bharoto, Eny E.; Yasa, I. Wayan W.; Herliana, Inna; Laksono, Brigitta; Xu, Kemin; Skepner, Eugene; Russell, Colin A.; Rimmelzwaan, Guus F.; Perez, Daniel R.; Osterhaus, Albert D. M. E.; Smith, Derek J.; Prajitno, Teguh Y.; Fouchier, Ron A. M.

2014-06-10T23:59:59.000Z

297

Development and Characterization of A Multiplexed RT-PCR Species Specific Assay for Bovine and one for Porcine Foot-and-Mouth Disease Virus Rule-Out  

SciTech Connect (OSTI)

Lawrence Livermore National Laboratory (LLNL), in collaboration with the Department of Homeland Security (DHS) and the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Services (APHIS) has developed candidate multiplexed assays that may potentially be used within the National Animal Health Laboratory Network (NAHLN), the National Veterinary Services Laboratory (Ames, Iowa) and the Plum Island Animal Disease Center (PIADC). This effort has the ability to improve our nation's capability to discriminate between foreign animal diseases and those that are endemic using a single assay, thereby increasing our ability to protect food and agricultural resources with a diagnostic test which could enhance the nation's capabilities for early detection of a foreign animal disease. In FY2005 with funding from the DHS, LLNL developed the first version (Version 1.0) of a multiplexed (MUX) nucleic-acid-based RT-PCR assay that included signatures for foot-and-mouth disease virus (FMDV) detection with rule-out tests for two other foreign animal diseases (FADs) of swine, Vesicular Exanthema of Swine (VESV) and Swine Vesicular Disease Virus (SVDV), and four other domestic viral diseases Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BHV-1), Bluetongue virus (BTV) and Parapox virus complex (which includes Bovine Papular Stomatitis Virus [BPSV], Orf of sheep, and Pseudocowpox). In FY06, LLNL has developed Bovine and Porcine species-specific panel which included existing signatures from Version 1.0 panel as well as new signatures. The MUX RT-PCR porcine assay for detection of FMDV includes the FADs, VESV and SVD in addition to vesicular stomatitis virus (VSV) and porcine reproductive and respiratory syndrome (PRRS). LLNL has also developed a MUX RT-PCR bovine assay for detection of FMDV with rule out tests for the two bovine FADs malignant catarrhal fever (MCF), rinderpest virus (RPV) and the domestic diseases vesicular stomatitis virus (VSV), bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitus virus (BHV-1), bluetongue virus (BTV), and the Parapox viruses (which are of two bovine types) bovine papular stomatitis virus (BPSV) and psuedocowpox (PCP). A timeline for this development is presented in Table 1. The development of the Version 1.0 panel for FMDV rule-out and the most current efforts aimed to designed species specific panels has spanned over 2 1/2 years with multiple collaborative partnerships. This document provides a summary of the development, testing and performance data at OIE Stage 1 Feasibility into Stage 2 Assay Development and Standardization1 (see Table 2), gathered as of June 30th, 2007 for the porcine and bovine MUX assay panels. We present an overview of the identification and selection of candidate genetic signatures, the assay development process, and preliminary performance data for each of the individual signatures as characterized in the multiplexed format for the porcine and bovine panels. The Stage 1 Feasibility data of the multiplexed panels is presented in this report also includes relevant data acquired from the Version 1.0 panel as supporting information where appropriate. In contrast to last years effort, the development of the bovine and porcine panels is pending additional work to complete analytical characterization of FMDV, VESV, SVD, RPV and MCF. The signature screening process and final panel composition impacts this effort. The unique challenge presented this year was having strict predecessor limitations in completing characterization, where efforts at LLNL must precede efforts at PIADC, such challenges were alleviated in the 2006 reporting by having characterization data from the interlaboratory comparison and at Plum Island under AgDDAP project. We will present an addendum at a later date with additional data on the characterization of the porcine and bovine multiplex assays when that data is available. As a summary report, this document does not provide the details of signature generation, evaluation, and testing, nor does it provide spec

Smith, S M; Danganan, L; Tammero, L; Vitalis, B; Lenhoff, R; Naraghi-arani, P; Hindson, B

2007-08-06T23:59:59.000Z

298

Real-Time Digital Flu Surveillance using Twitter Data Ankit Agrawal  

E-Print Network [OSTI]

place people turn for health information. People most often search for a specific disease or medical- 0938000, CCF-1043085, CCF-1029166, and OCI-1144061, and in part by DOE grants DE-FG02-08ER25848, DE-SC0001283, DE- SC0005309, DE-SC0005340, and DE-SC0007456. there is usually 1-2 weeks time lag before

299

Managing in the Home How to protect yourself and care for your family with the flu  

E-Print Network [OSTI]

with a waterproof dressing. 2. Cover your coughs & sneezes If you or members of your family are coughing or sneezing when you cough or sneeze and always put your used tissue into a rubbish tin. If you don't have a tissue, cough or sneeze into your upper sleeve, not your hands. Don't spit, and cover your mouth

Hickman, Mark

300

This factsheet gives advice about what to do if you think you have swine flu  

E-Print Network [OSTI]

F) or above, and some of the following: cough, sore throat, headache, tiredness, aching muscles, aching joints

Davies, Christopher

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

Putting Pandemics in Perspective: England and the Flu, 1889-1919  

E-Print Network [OSTI]

Authors who examine the Influenza Pandemic of 1918-19 fail to grasp its full context. Placing it alongside the Great War or other diseases only provides a partial construction, dramatically altering the narrative. With ...

Adams, David Lynn

2008-01-01T23:59:59.000Z

302

FLU ALERT SPRING 2014 WHAT CAN I DO TO STAY HEALTHY?  

E-Print Network [OSTI]

. When coughing or sneezing, cover your mouth and nose with a tissue or your inside elbow. It may prevent

Rhode Island, University of

303

1.11.13 "Oh no--I think I have the flu! Now what?!"  

E-Print Network [OSTI]

your hands often with soap and water, especially after you cough or sneeze. Alcohol-based hand cleaners are also effective. Cough or sneeze into your elbow, or use a tissue. Avoid touching your eyes, nose

Bushman, Frederic

304

H1N1 INFLUENZA (FLU) and INSTRUCTORS Version: 2.0 Page 1 of 3  

E-Print Network [OSTI]

to person when germs released from coughing and sneezing enter the nose and / or throat. Germs can also rest) or higher, a cough and one or more of the following: sore throat, muscle aches, joint pain, headaches (MHHL) recommends the following precautions: · Cover your cough by coughing into your elbow or sleeve

Major, Arkady

305

Flu is a serious contagious disease. Each year in the United States, on average, more  

E-Print Network [OSTI]

include: fever (usually high) headache extreme tiredness dry cough runny or stuffy nose muscle aches with a tissue when you cough or sneeze. Throw the tissue in the trash after you use it. Wash your hands often with soap and water, especially after you cough or sneeze. Alcohol-based hand cleaners are also effective

Ferrara, Katherine W.

306

H1N1 INFLUENZA (FLU) and YOU Version: 2.0 Page 1 of 2  

E-Print Network [OSTI]

to person when germs released from coughing and sneezing enter the nose and / or throat. Germs can also rest: Sudden Fever (100.4ºF / 38ºC); Cough; Runny nose; Sore throat; Body aches; Fatigue / physical Living (MHHL) recommends the following precautions: Cover your cough by coughing into your elbow

Major, Arkady

307

Decision ChartProtecting Yourself, Protecting Others IF YOU HAVE FLU SYMPTOMS  

E-Print Network [OSTI]

measures to avoid contamination: > Wash your hands frequently. > Cough or sneeze into the crook of your these symptoms: > Sore throat > Stuffy nose > Runny nose > Cough SITUATION FOR AN ADULT OR CHILD The person has a fever over 38°C (100.4°F). The fever came on suddenly and is accompanied by these symptoms: > Cough

308

E-Print Network 3.0 - avian h5n1 flu Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

possibility that it may begin... in wild birds H5N1 in birds - deadly to domestic poultry - other wild ... Source: Stowell, Michael - Department of Molecular, Cellular, and...

309

Towards understanding of Nipah virus attachment protein assembly and the role of protein affinity and crowding for membrane curvature events.  

SciTech Connect (OSTI)

Pathogenic viruses are a primary threat to our national security and to the health and economy of our world. Effective defense strategies to combat viral infection and spread require the development of understanding of the mechanisms that these pathogens use to invade the host cell. We present in this report results of our research into viral particle recognition and fusion to cell membranes and the role that protein affinity and confinement in lipid domains plays in membrane curvature in cellular fusion and fission events. Herein, we describe 1) the assembly of the G attachment protein of Nipah virus using point mutation studies to define its role in viral particle fusion to the cell membrane, 2) how lateral pressure of membrane bound proteins induce curvature in model membrane systems, and 3) the role of membrane curvature in the selective partitioning of molecular receptors and specific affinity of associated proteins.

Stachowiak, Jeanne C.; Hayden, Carl C.; Negrete, Oscar A.; Davis, Ryan Wesley; Sasaki, Darryl Yoshio

2013-10-01T23:59:59.000Z

310

CD8+ T cell antiviral activity: mechanism of induction and the suppression of emerging feline immunodeficiency virus strains  

E-Print Network [OSTI]

........................................ 93 12. Absolute CD8 + T lymphocyte counts until 11 weeks after infection of cats with varying doses of FIV-TX53 virus........................................ 94 13. Patterns of CD8? low expression... translation product of the FIV env gene is processed through proteolytic cleavage into the surface protein and the transmembrane protein (TM) (gp40) (Verschoor et al., 1993). Pancino et al. (1993b) have identified nine variable (V) regions throughout...

Phadke, Anagha

2007-09-17T23:59:59.000Z

311

The role of quail bronchitis virus as a possible precipitating factor in "air sac syndrome" of chickens  

E-Print Network [OSTI]

is caused by a filterable virus which is contagious and spreads rapidly throughout a flock. Age does not confer immunity. In infected chickens under six weeks, respiratory signs include gasping, coughing, and sneezing. Nasal discharges are:commonly sean... having a respiratory infection with a high mortality rate. Losses in some pens were 70% to 809o' and respiratory signs ranged from slight rales to coughing. There was no nasal discharge. Nervous signs were seen in a few of the infected quail...

Payne, Jerry Bob

1965-01-01T23:59:59.000Z

312

The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex  

SciTech Connect (OSTI)

Chlorella variabilis NC64A, a unicellular photosynthetic green alga (Trebouxiophyceae), is an intracellular photobiont of Paramecium bursaria and a model system for studying virus/algal interactions. We sequenced its 46-Mb nuclear genome, revealing an expansion of protein families that could have participated in adaptation to symbiosis. NC64A exhibits variations in GC content across its genome that correlate with global expression level, average intron size, and codon usage bias. Although Chlorella species have been assumed to be asexual and nonmotile, the NC64A genome encodes all the known meiosis-specific proteins and a subset of proteins found in flagella. We hypothesize that Chlorella might have retained a flagella-derived structure that could be involved in sexual reproduction. Furthermore, a survey of phytohormone pathways in chlorophyte algae identified algal orthologs of Arabidopsis thaliana genes involved in hormone biosynthesis and signaling, suggesting that these functions were established prior to the evolution of land plants. We show that the ability of Chlorella to produce chitinous cell walls likely resulted from the capture of metabolic genes by horizontal gene transfer from algal viruses, prokaryotes, or fungi. Analysis of the NC64A genome substantially advances our understanding of the green lineage evolution, including the genomic interplay with viruses and symbiosis between eukaryotes.

Blanc, Guillaume; Duncan, Garry A.; Agarakova, Irina; Borodovsky, Mark; Gurnon, James; Kuo, Alan; Lindquist, Erika; Lucas, Susan; Pangailinan, Jasmyn; Polle, Juergen; Salamov, Asaf; Terry, Astrid; Yamada, Takashi; Dunigan, David D.; Grigoriev, Igor V.; Claverie, Jean-Michel; Etten, James L. Van

2010-05-06T23:59:59.000Z

313

Elevated temperature triggers human respiratory syncytial virus F protein six-helix bundle formation  

SciTech Connect (OSTI)

Human respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infection in infants, immunocompromised patients, and the elderly. The RSV fusion (F) protein mediates fusion of the viral envelope with the target cell membrane during virus entry and is a primary target for antiviral drug and vaccine development. The F protein contains two heptad repeat regions, HR1 and HR2. Peptides corresponding to these regions form a six-helix bundle structure that is thought to play a critical role in membrane fusion. However, characterization of six-helix bundle formation in native RSV F protein has been hindered by the fact that a trigger for F protein conformational change has yet to be identified. Here we demonstrate that RSV F protein on the surface of infected cells undergoes a conformational change following exposure to elevated temperature, resulting in the formation of the six-helix bundle structure. We first generated and characterized six-helix bundle-specific antibodies raised against recombinant peptides modeling the RSV F protein six-helix bundle structure. We then used these antibodies as probes to monitor RSV F protein six-helix bundle formation in response to a diverse array of potential triggers of conformational changes. We found that exposure of 'membrane-anchored' RSV F protein to elevated temperature (45-55 deg. C) was sufficient to trigger six-helix bundle formation. Antibody binding to the six-helix bundle conformation was detected by both flow cytometry and cell-surface immunoprecipitation of the RSV F protein. None of the other treatments, including interaction with a number of potential receptors, resulted in significant binding by six-helix bundle-specific antibodies. We conclude that native, untriggered RSV F protein exists in a metastable state that can be converted in vitro to the more stable, fusogenic six-helix bundle conformation by an increase in thermal energy. These findings help to better define the mechanism of RSV F-mediated membrane fusion and have important implications for the identification of therapeutic strategies and vaccines targeting RSV F protein conformational changes.

Yunus, Abdul S.; Jackson, Trent P.; Crisafi, Katherine; Burimski, Irina; Kilgore, Nicole R.; Zoumplis, Dorian; Allaway, Graham P.; Wild, Carl T. [Panacos Pharmaceuticals, Inc., 209 Perry Parkway, Suite 7, Gaithersburg, MD 20877 (United States); Salzwedel, Karl, E-mail: salzwedelkd@niaid.nih.go [Panacos Pharmaceuticals, Inc., 209 Perry Parkway, Suite 7, Gaithersburg, MD 20877 (United States)

2010-01-20T23:59:59.000Z

314

The cowpox virus fusion regulator proteins SPI-3 and hemagglutinin interact in infected and uninfected cells  

SciTech Connect (OSTI)

The serpin SPI-3 and the hemagglutinin (HA) encoded by cowpox virus (CPV) block cell-cell fusion, and colocalize at the cell surface. wtCPV does not fuse cells, but inactivation of either gene leads to fusion. SPI-3 mAb added to wtCPV-infected cells caused fusion, confirming that SPI-3 protein at the cell surface prevents fusion. The SPI-3 mAb epitope mapped to an 85-amino acid region at the C-terminus. Removal of either 44 residues from the SPI-3 C-terminus or 48 residues following the N-terminal signal sequence resulted in fusion. Interaction between SPI-3 and HA proteins in infected cells was shown by coimmunoprecipitation. SPI-3/HA was not associated with the A27L 'fusion' protein. SPI-3 and HA were able to associate in uninfected cells in the absence of other viral proteins. The HA-binding domain in SPI-3 resided in the C-terminal 229 residues, and did not include helix D, which mediates cofactor interaction in many other serpins.

Turner, Peter C. [Department of Molecular Genetics and Microbiology, Box 100266/1600 SW Archer Road, ARB R2-231, University of Florida, Gainesville, FL 32610-0266 (United States)]. E-mail: pturner@mgm.ufl.edu; Moyer, Richard W. [Department of Molecular Genetics and Microbiology, Box 100266/1600 SW Archer Road, ARB R2-231, University of Florida, Gainesville, FL 32610-0266 (United States)]. E-mail: rmoyer@ufl.edu

2006-03-30T23:59:59.000Z

315

Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors  

SciTech Connect (OSTI)

Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of five influenza-vaccinated volunteers, with newly developed murine-human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: one for influenza A H1N1, four for influenza A H3N2 and five for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and four (two for H3N2 and two for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the two broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains.

Kubota-Koketsu, Ritsuko; Mizuta, Hiroyuki [Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871 (Japan)] [Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871 (Japan); Oshita, Masatoshi; Ideno, Shoji [Osaka Research Laboratory, Benesis Corporation, Yodogawa-ku, Osaka 532-6505 (Japan)] [Osaka Research Laboratory, Benesis Corporation, Yodogawa-ku, Osaka 532-6505 (Japan); Yunoki, Mikihiro [Osaka Research Laboratory, Benesis Corporation, Yodogawa-ku, Osaka 532-6505 (Japan) [Osaka Research Laboratory, Benesis Corporation, Yodogawa-ku, Osaka 532-6505 (Japan); Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871 (Japan); Kuhara, Motoki [Ina Laboratory, Medical and Biological Laboratories Corporation, Ltd., Ina, Nagano 396-0002 (Japan)] [Ina Laboratory, Medical and Biological Laboratories Corporation, Ltd., Ina, Nagano 396-0002 (Japan); Yamamoto, Naomasa [Department of Biochemistry, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima 963-8611 (Japan)] [Department of Biochemistry, School of Pharmaceutical Sciences, Ohu University, Koriyama, Fukushima 963-8611 (Japan); Okuno, Yoshinobu [Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa 768-0061 (Japan)] [Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa 768-0061 (Japan); Ikuta, Kazuyoshi, E-mail: ikuta@biken.osaka-u.ac.jp [Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871 (Japan)] [Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871 (Japan)

2009-09-11T23:59:59.000Z

316

The role of packaging sites in efficient and specific virus assembly  

E-Print Network [OSTI]

During the lifecycle of many single-stranded RNA viruses, including many human pathogens, a protein shell called the capsid spontaneously assembles around the viral genome. Understanding the mechanisms by which capsid proteins selectively assemble around the viral RNA amidst diverse host RNAs is a key question in virology. In one proposed mechanism, sequence elements (packaging sites) within the genomic RNA promote rapid and efficient assembly through specific interactions with the capsid proteins. In this work we develop a coarse-grained particle-based computational model for capsid proteins and RNA which represents protein-RNA interactions arising both from non-specific electrostatics and specific packaging sites interactions. Using Brownian dynamics simulations, we explore how the efficiency and specificity of assembly depend on solution conditions (which control protein-protein and nonspecific protein-RNA interactions) as well as the strength and number of packaging sites. We identify distinct regions in parameter space in which packaging sites lead to highly specific assembly via different mechanisms, and others in which packaging sites lead to kinetic traps. We relate these computational predictions to in vitro assays for specificity in which cognate viral RNAs are compete against non-cognate RNAs for assembly by capsid proteins.

J. D. Perlmutter; M. F. Hagan

2015-02-17T23:59:59.000Z

317

Terminal structures of West Nile virus genomic RNA and their interactions with viral NS5 protein  

SciTech Connect (OSTI)

Genome cyclization is essential for flavivirus replication. We used RNases to probe the structures formed by the 5'-terminal 190 nucleotides and the 3'-terminal 111 nucleotides of the West Nile virus (WNV) genomic RNA. When analyzed individually, the two RNAs adopt stem-loop structures as predicted by the thermodynamic-folding program. However, when mixed together, the two RNAs form a duplex that is mediated through base-pairings of two sets of RNA elements (5'CS/3'CSI and 5'UAR/3'UAR). Formation of the RNA duplex facilitates a conformational change that leaves the 3'-terminal nucleotides of the genome (position - 8 to - 16) to be single-stranded. Viral NS5 binds specifically to the 5'-terminal stem-loop (SL1) of the genomic RNA. The 5'SL1 RNA structure is essential for WNV replication. The study has provided further evidence to suggest that flavivirus genome cyclization and NS5/5'SL1 RNA interaction facilitate NS5 binding to the 3' end of the genome for the initiation of viral minus-strand RNA synthesis.

Dong Hongping; Zhang Bo [Wadsworth Center, New York State Department of Health, Albany, New York 12201 (United States); Shi Peiyong [Wadsworth Center, New York State Department of Health, Albany, New York 12201 (United States); Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, New York 12201 (United States)], E-mail: pei_yong.shi@novartis.com

2008-11-10T23:59:59.000Z

318

Evolutionary dynamics of endogenous feline leukemia virus proliferation among species of the domestic cat lineage  

SciTech Connect (OSTI)

Endogenous feline leukemia viruses (enFeLVs) occur in the germ lines of the domestic cat and related wild species (genus Felis). We sequenced the long terminal repeats and part of the env region of enFeLVs in domestic cats and five wild species. A total of 305 enFeLV sequences were generated across 17 individuals, demonstrating considerable diversity within two major clades. Distinct proliferations of enFeLVs occurred before and after the black-footed cat diverged from the other species. Diversity of enFeLVs was limited for the sand cat and jungle cat suggesting that proliferation of enFeLVs occurred within these species after they diverged. Relationships among enFeLVs were congruent with host species relationships except for the jungle cat, which carried only enFeLVs from a lineage that recently invaded the germline (enFeLV-AGTT). Comparison of wildcat and domestic cat enFeLVs indicated that a distinctive germ line invasion of enFeLVs has not occurred since the cat was domesticated.

Polani, Sagi, E-mail: sagi.polani@gmail.co [Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, 76100 (Israel); Roca, Alfred L., E-mail: roca@illinois.ed [Department of Animal Sciences and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 (United States); Rosensteel, Bryan B., E-mail: bryanr1@umbc.ed [University of Maryland, Baltimore, MD 21201 (United States); Kolokotronis, Sergios-Orestis, E-mail: koloko@amnh.or [Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY 10024 (United States); Bar-Gal, Gila Kahila, E-mail: bargal@agri.huji.ac.i [Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, 76100 (Israel)

2010-09-30T23:59:59.000Z

319

Hyaluronan Synthesis in Virus PBCV-1-Infected Chlorella-like Green Algae1 Michael V. Graves,* Dwight E. Burbank,* Robyn Roth, John Heuser, Paul L. DeAngelis, and James L. Van Etten*,2  

E-Print Network [OSTI]

Hyaluronan Synthesis in Virus PBCV-1-Infected Chlorella-like Green Algae1 Michael V. Graves previously reported that the chlorella virus PBCV-1 genome encodes an authentic, membrane-associated glycosyl as hyaluronan-lyase sensitive, hair-like fibers on the outside of the chlorella cell wall by 15­30 min

Graves, Michael V.

320

Environews Innovations A 468 VOLUME 113 | NUMBER 7 | July 2005 Environmental Health Perspectives  

E-Print Network [OSTI]

Environews Innovations A 468 VOLUME 113 | NUMBER 7 | July 2005 · Environmental Health Perspectives to existing treatments, such as chloroquine. Many experts believe that better repellents could help to control" with their antennae. Innovations | Outsmarting Olfaction #12;A 470 VOLUME 113 | NUMBER 7 | July 2005 · Environmental

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to obtain the most current and comprehensive results.


321

Induction of T helper 3 regulatory cells by dendritic cells infected with porcine reproductive and respiratory syndrome virus  

SciTech Connect (OSTI)

Delayed development of virus-specific immune response has been observed in pigs infected with the porcine reproductive and respiratory syndrome virus (PRRSV). Several studies support the hypothesis that the PRRSV is capable of modulating porcine immune system, but the mechanisms involved are yet to be defined. In this study, we evaluated the induction of T regulatory cells by PRRSV-infected dendritic cells (DCs). Our results showed that PRRSV-infected DCs significantly increased Foxp3{sup +}CD25{sup +} T cells, an effect that was reversible by IFN-alpha treatment, and this outcome was reproducible using two distinct PRRSV strains. Analysis of the expressed cytokines suggested that the induction of Foxp3{sup +}CD25{sup +} T cells is dependent on TGF-beta but not IL-10. In addition, a significant up-regulation of Foxp3 mRNA, but not TBX21 or GATA3, was detected. Importantly, our results showed that the induced Foxp3{sup +}CD25{sup +} T cells were able to suppress the proliferation of PHA-stimulated PBMCs. The T cells induced by the PRRSV-infected DCs fit the Foxp3{sup +}CD25{sup +} T helper 3 (Th3) regulatory cell phenotype described in the literature. The induction of this cell phenotype depended, at least in part, on PRRSV viability because IFN-alpha treatment or virus inactivation reversed these effects. In conclusion, this data supports the hypothesis that the PRRSV succeeds to establish and replicate in porcine cells early post-infection, in part, by inducing Th3 regulatory cells as a mechanism of modulating the porcine immune system.

Silva-Campa, Erika; Flores-Mendoza, Lilian; Resendiz, Monica; Pinelli-Saavedra, Araceli; Mata-Haro, Veronica [Centro de Investigacion en Alimentacion y Desarrollo, A.C. Hermosillo, Sonora (Mexico); Mwangi, Waithaka [Department of Veterinary Pathobiology, Texas A and M University, College Station, TX (United States); Hernandez, Jesus, E-mail: jhdez@ciad.m [Centro de Investigacion en Alimentacion y Desarrollo, A.C. Hermosillo, Sonora (Mexico)

2009-05-10T23:59:59.000Z

322

Discovery of a Novel Compound with Anti-Venezuelan Equine Encephalitis Virus Activity That Targets the Nonstructural Protein 2  

E-Print Network [OSTI]

W. Noah4, Chad E. Schroeder5, Julie B. Sotsky2, Melinda I. Sosa4, Daniel E. Cramer2, Sara N. McKellip4, Lynn Rasmussen4, E. Lucile White4, Connie S. Schmaljohn6, Justin G. Julander7, Jeffrey M. Smith6, Claire Marie Filone8, John H. Connor8, Yasuteru... infection. Citation: Chung D-H, Jonsson CB, Tower NA, Chu Y-K, Sahin E, et al. (2014) Discovery of a Novel Compound with Anti-Venezuelan Equine Encephalitis Virus Activity That Targets the Nonstructural Protein 2. PLoS Pathog 10(6): e1004213. doi:10...

Chung, Dong-Hoon; Jonsson, Colleen B.; Tower, Nichole A.; Chu, Yong-Kyu; Sahin, Ergin; Golden, Jennifer E.; Noah, James W.; Schroeder, Chad E.; Stosky, Julie B.; Sosa, Melinda; Cramer, Daniel E.; McKellip, Sara N.; Rasmussen, Lynn; White, E. Lucile; Schmaljohn, Connie S.; Julander, Justin G.; Smith, Jeffery M.; Filone, Claire Marie; Connor, John H.; Sakurai, Yasuteru; Davey, Robert A.

2014-06-26T23:59:59.000Z

323

Structure of the Ulster Strain Newcastle Disease Virus Hemagglutinin-Neuraminidase Reveals Auto-Inhibitory Interactions Associated with Low Virulence  

SciTech Connect (OSTI)

Paramyxovirus hemagglutinin-neuraminidase (HN) plays roles in viral entry and maturation, including binding to sialic acid receptors, activation of the F protein to drive membrane fusion, and enabling virion release during virus budding. HN can thereby directly influence virulence and in a subset of avirulent Newcastle disease virus (NDV) strains, such as NDV Ulster, HN must be proteolytically activated to remove a C-terminal extension not found in other NDV HN proteins. Ulster HN is 616 amino acids long and the 45 amino acid C-terminal extension present in its precursor (HN0) form has to be cleaved to render HN biologically active. Here we show that Ulster HN contains an inter-subunit disulfide bond within the C-terminal extension at residue 596, which regulates HN activities and neuraminidase (NA) domain dimerization. We determined the crystal structure of the dimerized NA domain containing the C-terminal extension, which extends along the outside of the sialidase {beta}-propeller domain and inserts C-terminal residues into the NA domain active site. The C-terminal extension also engages a secondary sialic acid binding site present in NDV HN proteins, which is located at the NA domain dimer interface, that most likely blocks its attachment function. These results clarify how the Ulster HN C-terminal residues lead to an auto-inhibited state of HN, the requirement for proteolytic activation of HN{sub 0} and associated reduced virulence.

Yuan, Ping; Paterson, Reay G.; Leser, George P.; Lamb, Robert A.; Jardetzky, Theodore S. (Stanford-MED); (NWU)

2012-09-06T23:59:59.000Z

324

Passive Infusion of Immune Serum into Simian Immunodeficiency Virus-Infected Rhesus Macaques Undergoing a Rapid Disease Course Has Minimal Effect on Plasma Viremia  

E-Print Network [OSTI]

Passive Infusion of Immune Serum into Simian Immunodeficiency Virus-Infected Rhesus Macaques of passively infused antibody on plasma viremia in infected rhesus macaques. The emphasis of the study was to understand the mechanism(s) underlying any observed effects. We infused serum immunoglobulins (SIVIG

Bonhoeffer, Sebastian

325

Principal maize viruses in Mediterranean countries D Ivanovi&jadnr; R Osler N Katis M Ivanovi&jadnr; D lgnjatovi&jadnr;  

E-Print Network [OSTI]

yellow dwarf viruses (BYDVs) (PAV- and RPV-like) were used in these tests. A higher disease incidence.7 and 11 % of individual Greek samples were positive for PAV- and RPV- respectively, while, 17.5 and 5% of Yugoslav samples were positive for PAV- and RPV- respectively. Phragmites sp, a perennial maize weed

Boyer, Edmond

326

Professor Jotun Hein "Comparative Virus Annotation" Comparative Genomics has exploded in recent years and put the techniques of molecular evolution at  

E-Print Network [OSTI]

Professor Jotun Hein "Comparative Virus Annotation" Comparative Genomics has exploded in recent information from sequence data. Originally, sequences/genomes were obtained from a single species at a time, but as more genomes have been determined, it has become apparent that the true benefit of this enterprise lies

Goldschmidt, Christina

327

1292 IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, VOL. 12, NO. 6, NOVEMBER/DECEMBER 2006 Optical Detection of Single Nanoparticles and Viruses  

E-Print Network [OSTI]

on a nanoparticle as it passes through a confined optical field, and the other method uses a background, sensor, viruses. I. INTRODUCTION THERE is a high demand for sensors that are able to detect small amounts this threat. Therefore, a broad network of sensors has to be deployed. These sensors must be affordable

Novotny, Lukas

328

Critical Role of the 36-nucleotide Insertion in Hepatitis B Virus Genotype G on Core Protein Expression, Genome Replication, and Virion Secretion  

E-Print Network [OSTI]

Critical Role of the 36-nucleotide Insertion in Hepatitis B Virus Genotype G on Core Protein in the core protein due to a 36-nucleotide insertion near the core gene translation initiation codon transcription, genome replication, and virion secretion. Interestingly, the 36-nucleotide insertion markedly

Paris-Sud XI, Université de

329

Development of an in vitro procedure to eradicate potato viruses X, Y, and S from the potato (Solanum tuberosum L.) variety Russet Norko two of its strains  

E-Print Network [OSTI]

A tissue culture method was developed for the eradication of three of the most important potato viruses': PVX, PVY, and PVS from the Russet Norkotah variety and two of its strains (TXNS 278 and TXNS 112). The method combined the use of liquid medium...

Zapata Carrero, Carmen Cecilia

1994-01-01T23:59:59.000Z

330

Influence of the host cell factors CK2, hTERT, and PML, on the antiviral response to herpes simplex virus type I infection  

E-Print Network [OSTI]

deploys a variety of defenses to limit the extent to which the virus can replicate. This thesis is the summation of projects that examined the impact of three host cell factors - those being CK2, hTERT, and PML - on HSV-1 replication. CK2 is a cellular...

Smith, Miles Christian

2013-08-31T23:59:59.000Z

331

Journal of Leukocyte Biology 47:332-343 (1990) Influenza A Virus Alters Structural and Biochemical Functions of the Neutrophil Cytoskeleton  

E-Print Network [OSTI]

Influenza A virus (lAy) has previousiy been shown to alter chemotactic, oxidative, and secretory functIons of polymorphonuclear leukocytes (PMNL). Because of the role of cytoskeletal proteins In these processes, studies were carried out to determine if IAV altered the PMNL cytoskeleton. PMNL were incubated with buffer or lAy, stimulated with f-met-Ieu-phe (FMLP), fixed and stained with NBD-phallacldln (NBD-Ph) and studied by flow cytometry. Mean F-actin fluorescence was increased 18 % in virus treated cells pre-FMLP stimulation and 13 % Sand 10 mm post-FMLP (P <.03); no significant difference in F-actln fluorescence was noted in virus treated PMNL 15-30 a post-FMLP compared to control cells. PMNL exposed to the same conditions were solubilized and actin content was determined following SDS-PAGE of triton insoluble precipitates. Increased actln was recovered from virus treated compared to buffer treated cells before and after FMLP stImulation in the 8,0009 precipitates (P <.001). Immunofluorescent microscopy studies of F-actln distribution were done in PMNL stained with NBD-Ph following FMLP stimulation for 10 mm. These studies showed an increased lamellipod F-actin/uropod F-actin ratio in PMNL pre-incubated with IAV compared to controls (4.6 vs. 1.0; P <.025). Phosphorylation of specific cytoskeletal proteins was examined after immunoprecipitation. IAV alone altered phosphorylation of both vimentin and vinculin, and in stimulated PMNL virus led to decreased phosphorylation of vimentin and vinculin. These data show distributional and biochemical effects of IAV on PMNL cytoskeietal proteins, indicating additional targets for IAV interference in the PMNL sIgnal-transduction-function process.

J. Gary Wheeler; Lisa S. Winkler; Michael Seeds; David Bass; Jon S. Abramson

332

Separation of Yeast Cells from MS2 Viruses Using Acoustic Radiation Force  

SciTech Connect (OSTI)

We report a rapid and robust separation of Saccharomyces cerevisiae and MS2 bacteriophage using acoustic focusing in a microfluidic device. A piezoelectric transducer (PZT) generates acoustic standing waves in the microchannel. These standing waves induce acoustic radiation force fields that direct microparticles towards the nodes (i.e., pressure minima) or the anti-nodes (i.e., pressure maxima) of the standing waves depending on the relative compressidensity between the particle and the suspending liquid.[1] For particles larger than 2 {micro}m, the transverse velocities generated by these force fields enable continuous, high throughput separation. Extensive work in the last decade [2-4] has demonstrated acoustic focusing for manipulating microparticles or biological samples in microfluidic devices. This prior work has primarily focused on experimental realization of acoustic focusing without modeling or with limited one-dimensional modeling estimates. We recently developed a finite element modeling tool to predict the two-dimensional acoustic radiation force field perpendicular to the flow direction in microfluidic devices.[1] Here we compare results from this model with experimental parametric studies including variations of the PZT driving frequencies and voltages as well as various particle sizes and compressidensities. These experimental parametric studies also provide insight into the development of an adjustable 'virtual' pore-size filter as well as optimal operating conditions for various microparticle sizes. Figure 1 shows a typical experimental acoustic focusing result for microparticles (diameter = 2.0 {micro}m) in a 500 {micro}m wide by 200 {micro}m deep microchannel. In this case, the PZT driving frequency and voltage are, respectively, 1.459 MHz and 6.6 V. The microparticles tightly focus (full width half maximum (FWHM) {approx}30 {micro}m) less than 30 s after the initiation of the acoustic field. We simulated the same geometry and operating conditions for comparison. The surface plot in Figure 2 illustrates the two-dimensional pressure field orthogonal to the flow direction (x-direction) from the simulation. The superimposed vector plot shows the acoustic radiation force in this plane. The dark regions and the light regions respectively represent the nodes and anti-nodes of the acoustic pressure field. The corresponding force field predicts acoustic focusing at the center of the microchannel, which is confirmed by the experimental results shown in Figure 1. We demonstrated the separation of Saccharomyces cerevisiae (typical cell size of 4-6 {micro}m depending on the cell growth stage, measured using a Coulter counter) and MS2 bacteriophage (typical diameter {approx}30 nm [5]) using acoustic focusing (Figure 3). A mixture of S. cerevisiae and MS2 labeled with Ribogreen was prepared and injected into one inlet of the microchip (i.e., half of the microchannel was filled with the sample). We varied driving voltages from 1.96 to 4.76 V, while fixing the driving frequency at 1.459 MHz and flow rate at 20 {micro}l/min. The acoustic radiation force did not affect the MS2 viruses, and their concentration profile remained unchanged. Increased driving voltages enhanced the acoustic focusing of the yeast cells thereby achieving good separation. We are able to achieve yields of > 80% and sample purities of > 90% in this continuous-flow sample preparation device.

Jung, B; Fisher, K; Ness, K; Rose, K A; Mariella, Jr., R P

2008-03-27T23:59:59.000Z

333

Localization of VP28 on the baculovirus envelope and its immunogenicity against white spot syndrome virus in Penaeus monodon  

SciTech Connect (OSTI)

White spot syndrome virus (WSSV) is a large dsDNA virus responsible for white spot disease in shrimp and other crustaceans. VP28 is one of the major envelope proteins of WSSV and plays a crucial role in viral infection. In an effort to develop a vaccine against WSSV, we have constructed a recombinant baculovirus with an immediate early promoter 1 which expresses VP28 at an early stage of infection in insect cells. Baculovirus expressed rVP28 was able to maintain its structural and antigenic conformity as indicated by immunofluorescence assay and western blot analysis. Interestingly, our results with confocal microscopy revealed that rVP28 was able to localize on the plasma membrane of insect cells infected with recombinant baculovirus. In addition, we demonstrated with transmission electron microscopy that baculovirus successfully acquired rVP28 from the insect cell membrane via the budding process. Using this baculovirus displaying VP28 as a vaccine against WSSV, we observed a significantly higher survival rate of 86.3% and 73.5% of WSSV-infected shrimp at 3 and 15 days post vaccination respectively. Quantitative real-time PCR also indicated that the WSSV viral load in vaccinated shrimp was significantly reduced at 7 days post challenge. Furthermore, our RT-PCR and immunohistochemistry results demonstrated that the recombinant baculovirus was able to express VP28 in vivo in shrimp tissues. This study will be of considerable significance in elucidating the morphogenesis of WSSV and will pave the way for new generation vaccines against WSSV.

Syed Musthaq, S.; Madhan, Selvaraj [Animal Health Biotechnology, Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, 117604 (Singapore); Sahul Hameed, A.S. [OIE Expert, OIE Reference Laboratory for WTD, C. Abdul Hakeem College, Melvisharam 632 509 (India); Kwang, Jimmy, E-mail: kwang@tll.org.s [Animal Health Biotechnology, Temasek Lifesciences Laboratory, 1 Research Link, National University of Singapore, 117604 (Singapore); Department of Microbiology, Faculty of Medicine, National University of Singapore (Singapore)

2009-09-01T23:59:59.000Z

334

Glycosyl-phosphatidylinositol (GPI)-anchored membrane association of the porcine reproductive and respiratory syndrome virus GP4 glycoprotein and its co-localization with CD163 in lipid rafts  

SciTech Connect (OSTI)

The porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 4 (GP4) resembles a typical type I membrane protein in its structure but lacks a hydrophilic tail at the C-terminus, suggesting that GP4 may be a lipid-anchored membrane protein. Using the human decay-accelerating factor (DAF; CD55), a known glycosyl-phosphatidylinositol (GPI) lipid-anchored protein, chimeric constructs were made to substitute the GPI-anchor domain of DAF with the putative lipid-anchor domain of GP4, and their membrane association and lipase cleavage were determined in cells. The DAF-GP4 fusion protein was transported to the plasma membrane and was cleaved by phosphatidylinositol-specific phospholipase C (PI-PLC), indicating that the C-terminal domain of GP4 functions as a GPI anchor. Mutational studies for residues adjacent to the GPI modification site and characterization of respective mutant viruses generated from infectious cDNA clones show that the ability of GP4 for membrane association corresponded to virus viability and growth characteristics. The residues T158 ({omega} - 2, where {omega} is the GPI moiety at E160), P159 ({omega} - 1), and M162 ({omega} + 2) of GP4 were determined to be important for virus replication, with M162 being of particular importance for virus infectivity. The complete removal of the peptide-anchor domain in GP4 resulted in a complete loss of virus infectivity. The depletion of cholesterol from the plasma membrane of cells reduced the virus production, suggesting a role of lipid rafts in PRRSV infection. Remarkably, GP4 was found to co-localize with CD163 in the lipid rafts on the plasma membrane. Since CD163 has been reported as a cellular receptor for PRRSV and GP4 has been shown to interact with this receptor, our data implicates an important role of lipid rafts during entry of the virus.

Du, Yijun [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States) [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States); Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan (China); Pattnaik, Asit K. [School of Veterinary Medicine and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583-0900 (United States)] [School of Veterinary Medicine and Biomedical Sciences and the Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583-0900 (United States); Song, Cheng [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States)] [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States); Yoo, Dongwan, E-mail: dyoo@illinois.edu [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States)] [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States); Li, Gang, E-mail: dyoo@illinois.edu [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States) [Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802 (United States); Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing (China)

2012-03-01T23:59:59.000Z

335

Development and Characterization of a Multiplexed RT-PCR Species Specific Assay for Bovine and one for Porcine Foot-and-Mouth Disease Virus Rule-Out Supplemental Materials  

SciTech Connect (OSTI)

Lawrence Livermore National Laboratory (LLNL), in collaboration with the Department of Homeland Security (DHS) and the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Services (APHIS) has developed advanced rapid diagnostics that may be used within the National Animal Health Laboratory Network (NAHLN), the National Veterinary Services Laboratory (Ames, Iowa) and the Plum Island Animal Disease Center (PIADC). This effort has the potential to improve our nation's ability to discriminate between foreign animal diseases and those that are endemic using a single assay, thereby increasing our ability to protect animal populations of high economic importance in the United States. Under 2005 DHS funding we have developed multiplexed (MUX) nucleic-acid-based PCR assays that combine foot-and-mouth disease virus (FMDV) detection with rule-out tests for two other foreign animal diseases Vesicular Exanthema of Swine (VESV) and Swine Vesicular Disease (SVD) and four other domestic viral diseases Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BHV-1 or Infectious Bovine Rhinotracheitus IBR), Bluetongue virus (BTV) and Parapox virus complex (which includes Bovine Papular Stomatitis Virus BPSV, Orf of sheep, and Pseudocowpox). Under 2006 funding we have developed a Multiplexed PCR [MUX] porcine assay for detection of FMDV with rule out tests for VESV and SVD foreign animal diseases in addition to one other domestic vesicular animal disease vesicular stomatitis virus (VSV) and one domestic animal disease of swine porcine reproductive and respiratory syndrome (PRRS). We have also developed a MUX bovine assay for detection of FMDV with rule out tests for the two bovine foreign animal diseases malignant catarrhal fever (MCF), rinderpest virus (RPV) and the domestic diseases vesicular stomatitis virus (VSV), bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitus virus (BHV-1), bluetongue virus (BTV), and the Parapox viruses which are of two bovine types bovine papular stomatitis virus (BPSV) and psuedocowpox (PCP). This document provides details of signature generation, evaluation, and testing, as well as the specific methods and materials used. A condensed summary of the development, testing and performance of the multiplexed assay panel was presented in a 126 page separate document, entitled 'Development and Characterization of A Multiplexed RT-PCR Species Specific Assay for Bovine and one for Porcine Foot-and-Mouth Disease Virus Rule-Out'. This supplemental document provides additional details of large amount of data collected for signature generation, evaluation, and testing, as well as the specific methods and materials used for all steps in the assay development and utilization processes. In contrast to last years effort, the development of the bovine and porcine panels is pending additional work to complete analytical characterization of FMDV, VESV, VSV, SVD, RPV and MCF. The signature screening process and final panel composition impacts this effort. The unique challenge presented this year was having strict predecessor limitations in completing characterization, where efforts at LLNL must preceed efforts at PIADC, such challenges were alleviated in the 2006 reporting by having characterization data from the interlaboratory comparison and at Plum Island under AgDDAP project. We will present an addendum at a later date with additional data on the characterization of the porcine and bovine multiplex assays when that data is available.

Smith, S; Danganan, L; Tammero, L; Lenhoff, R; Naraghi-arani, P; Hindson, B

2007-08-06T23:59:59.000Z

336

The effect of an experimental thymidine kinase negative bovine herpesvirus-I vaccine on virus shedding, antibody response, and latency in cattle  

E-Print Network [OSTI]

THE EFFECT OF AN EXPERIMENTAL THYMIDINE KINASE NEGATIVE BOVINE HERPESVIRUS-I VACCINE ON VIRUS SHEDDING, ANTIBODY RESPONSE, AND LATENCY IN CATTLE. A Thesis by PEGGY MCCARVER BILLINGSLEY Submitted to the Graduate College of Texas ASM University... in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE August 1985 Major Subject: Veterinary Microbiology THE EFFECT OF AN EXPERIMENTAL THYMIDINE KINASE NEGATIVE BOVINE HERPESVIRUS-I VACCINE ON SHEDDING, ANTIBODY RESPONSE...

Billingsley, Peggy McCarver

2012-06-07T23:59:59.000Z

337

Mutagenic analysis of herpes simplex virus type 1 glycoprotein L reveals the importance of an arginine-rich region for function  

SciTech Connect (OSTI)

Herpes simplex virus type 1 (HSV-1) glycoproteins H and L (gH and gL) are required for virus-induced membrane fusion. Expression of gH at the virion or infected cell surface is mediated by the chaperone-like activity of gL. We have previously shown that a region between amino acids 155 and 161 is critical for gL chaperone-like activity. Here, we conducted Ala substitution mutagenesis of residues in this region and found that substitution of Cys160, Arg156, Arg158, or Arg156/158/159 with Ala resulted in a gL mutant that bound gH but displayed a reduced ability in gH trafficking and membrane fusion. Substitution of Arg156 with another positively charged amino acid, Lys, restored function. Substitution of Arg158 with Lys restored function in gH trafficking and cell fusion but not virus entry. These results indicate that an arginine-rich region of gL is critical for function.

Klyachkin, Yuri M. [University of Kentucky, Department of Microbiology, Immunology, and Molecular Genetics, 800 Rose St., UKMC MS423, Lexington, KY 40536-0298 (United States); Geraghty, Robert J. [University of Kentucky, Department of Microbiology, Immunology, and Molecular Genetics, 800 Rose St., UKMC MS423, Lexington, KY 40536-0298 (United States)], E-mail: rgeragh@uky.edu

2008-04-25T23:59:59.000Z

338

Palmitoylation of the feline immunodeficiency virus envelope glycoprotein and its effect on fusion activity and envelope incorporation into virions  

SciTech Connect (OSTI)

The feline immunodeficiency virus (FIV) envelope glycoprotein (Env) possesses a short cytoplasmic domain of 53 amino acids containing four highly conserved cysteines at Env positions 804, 811, 815 and 848. Since palmitoylation of transmembrane proteins occurs at or near the membrane anchor, we investigated whether cysteines 804, 811 and 815 are acylated and analyzed the relevance of these residues for Env functions. Replacement of cysteines 804, 811 and 815 individually or in combination by serine residues resulted in Env glycoproteins that were efficiently expressed and processed. However, mutations C804S and C811S reduced Env fusogenicity by 93% and 84%, respectively, compared with wild-type Env. By contrast, mutant C815S exhibited a fusogenic capacity representing 50% of the wild-type value. Remarkably, the double mutation C804S/C811S abrogated both Env fusion activity and Env incorporation into virions. Finally, by means of Click chemistry assays we demonstrated that the four FIV Env cytoplasmic cysteines are palmitoylated.

Gonzalez, Silvia A.; Paladino, Monica G. [Laboratorio de Virologia, CONICET-Universidad de Belgrano (UB), Villanueva 1324 (C1426BMJ), Buenos Aires (Argentina)] [Laboratorio de Virologia, CONICET-Universidad de Belgrano (UB), Villanueva 1324 (C1426BMJ), Buenos Aires (Argentina); Affranchino, Jose L., E-mail: jose.affranchino@comunidad.ub.edu.ar [Laboratorio de Virologia, CONICET-Universidad de Belgrano (UB), Villanueva 1324 (C1426BMJ), Buenos Aires (Argentina)

2012-06-20T23:59:59.000Z

339

An integrated protein localization and interaction map for Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus  

SciTech Connect (OSTI)

The genome of Potato yellow dwarf virus (PYDV; Nucleorhabdovirus type species) was determined to be 12,875 nucleotides (nt). The antigenome is organized into seven open reading frames (ORFs) ordered 3'-N-X-P-Y-M-G-L-5', which likely encode the nucleocapsid, phospho, movement, matrix, glyco and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. The ORFs are flanked by a 3' leader RNA of 149 nt and a 5' trailer RNA of 97 nt, and are separated by conserved intergenic junctions. Phylogenetic analyses indicated that PYDV is closely related to other leafhopper-transmitted rhabdoviruses. Functional protein assays were used to determine the subcellular localization of PYDV proteins. Surprisingly, the M protein was able to induce the intranuclear accumulation of the inner nuclear membrane in the absence of any other viral protein. Finally, bimolecular fluorescence complementation was used to generate the most comprehensive protein interaction map for a plant-adapted rhabdovirus to date.

Bandyopadhyay, Anindya; Kopperud, Kristin; Anderson, Gavin; Martin, Kathleen [Department of Plant Pathology, University of Kentucky, Lexington, KY (United States); Goodin, Michael, E-mail: mgoodin@uky.ed [Department of Plant Pathology, University of Kentucky, Lexington, KY (United States)

2010-06-20T23:59:59.000Z

340

Mosquito larval habitat mapping using remote sensing and GIS: Implications of coalbed methane development and West Nile virus  

SciTech Connect (OSTI)

Potential larval habitats of the mosquito Culex tarsalis (Coquillett), implicated as a primary vector of West Nile virus in Wyoming, were identified using integrated remote sensing and geographic information system (GIS) analyses. The study area is in the Powder River Basin of north central Wyoming, an area that has been undergoing a significant increase in coalbed methane gas extractions since the late 1990s. Large volumes of water are discharged, impounded, and released during the extraction of methane gas, creating aquatic habitats that have the potential to support immature mosquito development. Landsat TM and ETM + data were initially classified into spectrally distinct water and vegetation classes, which were in turn used to identify suitable larval habitat sites. This initial habitat classification was refined using knowledge-based GIS techniques requiring spatial data layers for topography, streams, and soils to reduce the potential for overestimation of habitat. Accuracy assessment was carried out using field data and high-resolution aerial photography commensurate with one of the Landsat images. The classifier can identify likely habitat for ponds larger than 0.8 ha (2 acres) with generally satisfactory results (72.1%) with a lower detection limit of approximate to 0.4 ha (1 acre). Results show a 75% increase in potential larval habitats from 1999 to 2004 in the study area, primarily because of the large increase in small coalbed methane water discharge ponds. These results may facilitate mosquito abatement programs in the Powder River Basin with the potential for application throughout the state and region.

Zou, L.; Miller, S.N.; Schmidtmann, E.T. [University of Wyoming, Laramie, WY (United States). Dept. of Renewable Resources

2006-09-15T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Rapid and reversible reduction of junctional permeability in cells infected with a temperature-sensitive mutant of avian sarcoma virus  

E-Print Network [OSTI]

ABSTRACT The transformed or normal phenotype of cultured normal rat kidney cells infected with a temperature-sensitive mutant of avian sarcoma virus is conditional on the temperature at which the cells are grown. Using dye injection techniques, we show that junction-mediated dye transfer is also temperature-sensitive. The extent and rate of transfer between infected cells grown at the transformation-permissive temperature (35 C) is significantly reduced when compared to infected cells grown at the nonpermissive temperature (40.5 C) or uninfected cells grown at either temperature. Infected cells subjected to reciprocal temperature shifts express rapid and reversible alterations of dye transfer capacities, with responses evident by 15 min and completed by 60 min for temperature shifts in either direction. These results suggest that altered junctional capacities may be fundamental to the expression of the ASV-induced, transformed phenotype. Most cells possess permeable junctions which connect the interiors of adjacent cells and allow the direct exchange oflow molecular weight substances (for review, see references 1-3). When present, such junctions provide the capacity for direct electrical communication between electrically excitable cells

Michael M. Atkinson; A. S. Menko; Ross G. Johnson; J R Sheppard; Judson D. Sheridan

1981-01-01T23:59:59.000Z

342

What are the symptoms of influenza? Influenza is also known as "the flu." The most common symptoms are fever with  

E-Print Network [OSTI]

are fever with respiratory symptoms including cough, sore throat and body aches. Headaches, nausea, vomiting the clock;" frequency indicated on the product directions For cough: n Use a cough syrup cough n Breathing difficulty n Chest pain n Or other new severe symptoms Stay home from class and work

Taylor, Jerry

343

"When I have a cold or `flu' my mucus turns yellow. My mom always says that means I  

E-Print Network [OSTI]

, thicken, and discolor overnight from sitting in the head - showing color in the morning when you "cough medications to lessen symptoms. Runny nose, posterior nasal drainage (PND), cough, sore throat, and symptoms. Often runny nose and intermittent cough are the last symptoms to resolve taking 2 weeks. Some students

Virginia Tech

344

To: Wesleyan student, staff, faculty and parents: This year's flu season has occurred early and is widespread. In order  

E-Print Network [OSTI]

your hands with soap and water, especially after coughing or sneezing. Alcohol-based hand cleaners also and nose with a tissue when you cough or sneeze. If you don't have a tissue, cough or sneeze into your (chills, night sweats) accompanied by cough, body aches, sore throat, runny nose, headache, or fatigue

Devoto, Stephen H.

345

A single amino acid change resulting in loss of fluorescence of eGFP in a viral fusion protein confers fitness and growth advantage to the recombinant vesicular stomatitis virus  

SciTech Connect (OSTI)

Using a recombinant vesicular stomatitis virus encoding eGFP fused in-frame with an essential viral replication protein, the phosphoprotein P, we show that during passage in culture, the virus mutates the nucleotide C289 within eGFP of the fusion protein PeGFP to A or T, resulting in R97S/C amino acid substitution and loss of fluorescence. The resultant non-fluorescent virus exhibits increased fitness and growth advantage over its fluorescent counterpart. The growth advantage of the non-fluorescent virus appears to be due to increased transcription and replication activities of the PeGFP protein carrying the R97S/C substitution. Further, our results show that the R97S/C mutation occurs prior to accumulation of mutations that can result in loss of expression of the gene inserted at the G-L gene junction. These results suggest that fitness gain is more important for the recombinant virus than elimination of expression of the heterologous gene.

Dinh, Phat X.; Panda, Debasis; Das, Phani B.; Das, Subash C.; Das, Anshuman [School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States) [School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States); The Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States); Pattnaik, Asit K., E-mail: apattnaik2@unl.edu [School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States); The Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0900 (United States)

2012-10-25T23:59:59.000Z

346

Please cite this article in press as: White III, R.A., et al., Digital PCR provides absolute quantitation of viral load for an occult RNA virus. J. Virol. Methods (2011), doi:10.1016/j.jviromet.2011.09.017  

E-Print Network [OSTI]

without need for a standard curve. 2011 Published by Elsevier B.V. 1. Introduction20 Viral load testing quantitation of viral load for an occult RNA virus. J. Virol. Methods (2011), doi:10.1016/j.jviromet.2011.elsevier.com/locate/jviromet Digital PCR provides absolute quantitation of viral load for an occult RNA virus1 Richard Allen White IIIa

Quake, Stephen R.

2011-01-01T23:59:59.000Z

347

E-Print Network 3.0 - atypical chest pain Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

FLU SYMPTOMS Symptom Cold H1N1 Flu Summary: with the flu (sometimes referred to as dry cough) Aches Slight body aches and pains can be part of the cold... Severe aches and pains...

348

You give me fever : practical protection for metropolitan neuroses  

E-Print Network [OSTI]

The emergence of Swine Flu in the past six months has once again heightened the world's fears of a coming flu pandemic. Although H1N1 is only slightly more pathogenic than the common seasonal flu, which kills approximately ...

Case, Keith William

2010-01-01T23:59:59.000Z

349

Establishment of a Bovine Herpesvirus 4 based vector expressing a secreted form of the Bovine Viral Diarrhoea Virus structural glycoprotein E2 for immunization purposes  

E-Print Network [OSTI]

immunized with plasmid encoding a secreted form of gE2 developed significantly higher IgG and virus neutralizing antibody titres compared to animals vaccinated with plas- mid encoding a membrane linked gE2 [21]. Starting with this data, we successfully... (Roche). A Kanamycin expression cassette was then cut out with MluI from pMu- Kana vector [47], blunted end and ligated into pINT2GalK cut with PstI and blunted end, so that the pTK-KanaGalK- TK was generated. The second Targeting Vector, pTK-CMV-IgKE2...

Donofrio, Gaetano; Sartori, Chiara; Ravanetti, Lara; Cavirani, Sandro; Gillet, Laurent; Vanderplasschen, Alain; Taddei, Simone; Flammini, Cesidio Filippo

2007-10-18T23:59:59.000Z

350

The presence of tristeza virus in Satsuma oranges, Meyer lemons, and other citrus plants in the Gulf Coast areas in Texas  

E-Print Network [OSTI]

'TS I. !TOO/ 'JCT I "'I. EE Y I E OF L I TEA iT J RE ~ /nTE-I I/' r N YIETRJ" S. IES'J' T ' " '/' . ':I SC'J 'S I tnI Symptoms oF the isease in mexican Lime Seedlings. Citrus '/arieties Founc to Carry Tristeza "irus. = J / ?", RY ' N CC... on var i ous rootstocks may be symptomlsss carriers of tristsza virus (Glson ano Sleeth, 1/54) (7". ); (Olson and 'Jlacl onald, 1y54)(21). '!hi ls the major citrus area in Texas is the Lower Rio Grande Valley~ where the commor rootstock is sour...

Malouf, Wajih D

1959-01-01T23:59:59.000Z

351

In vivo subcellular localization of Mal de Rio Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions  

SciTech Connect (OSTI)

The in vivo subcellular localization of Mal de Rio Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloud-like inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and {alpha}-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread.

Maroniche, Guillermo A.; Mongelli, Vanesa C.; Llauger, Gabriela; Alfonso, Victoria; Taboga, Oscar [Instituto de Biotecnologia, CICVyA, Instituto Nacional de Tecnologia Agropecuaria (IB-INTA), Las cabanas y Los Reseros s/n. Hurlingham Cp 1686, Buenos Aires (Argentina)] [Instituto de Biotecnologia, CICVyA, Instituto Nacional de Tecnologia Agropecuaria (IB-INTA), Las cabanas y Los Reseros s/n. Hurlingham Cp 1686, Buenos Aires (Argentina); Vas, Mariana del, E-mail: mdelvas@cnia.inta.gov.ar [Instituto de Biotecnologia, CICVyA, Instituto Nacional de Tecnologia Agropecuaria (IB-INTA), Las cabanas y Los Reseros s/n. Hurlingham Cp 1686, Buenos Aires (Argentina)

2012-09-01T23:59:59.000Z

352

Joint environmental assessment 1997--2001 of the California Department of Food and Agriculture Curly Top Virus Control Program for Bureau of Land Management and Department of Energy  

SciTech Connect (OSTI)

The DOE, Naval Petroleum reserves in California (NPRC), proposes to sign an Amendment to the Cooperative Agreement and Supplement with the California Department of Food and Agriculture (CDFA) to extend the term of the Curly Top Virus Control Program (CTVCP) in California. This program involves Malathion spraying on NPRC lands to control the beet leafhopper, over a five year period from 1997 through 2001. It is expected that approximately 330 acres on Naval Petroleum Reserve Number 1 (NPR-1) and approximately 9,603 acres on Naval Petroleum Reserve Number 2 (NPR-2) will be treated with Malathion annually by CDFA during the course of this program. The actual acreage subject to treatment can vary from year to year. Pursuant to the requirements of the National Environmental Policy Act of 1969 (NEPA), as amended, the potential impacts of the proposed action were analyzed in a Joint Environmental Assessment (DOE/EA-1011) with the US Department of Interior, Bureau of Land Management (BLM) acting as lead agency, in consultation with the CDFA, and the DOE acting as a cooperating agency. Based on the analysis in the EA, DOE has determined that the conduct of the Curly Top Virus Control Program in California is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the NEPA. Therefore, the preparation of an Environmental Impact Statement is not required and DOE is consequently issuing a FONSI.

NONE

1997-03-01T23:59:59.000Z

353

Fully human broadly neutralizing monoclonal antibodies against influenza A viruses generated from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient  

SciTech Connect (OSTI)

Whether the 2009 pandemic H1N1 influenza vaccine can induce heterosubtypic cross-protective anti-hemagglutinin (HA) neutralizing antibodies is an important issue. We obtained a panel of fully human monoclonal antibodies from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient. Most of the monoclonal antibodies targeted the HA protein but not the HA1 fragment. Among the analyzed antibodies, seven mAbs exhibited neutralizing activity against several influenza A viruses of different subtypes. The conserved linear epitope targeted by the neutralizing mAbs (FIEGGWTGMVDGWYGYHH) is part of the fusion peptide on HA2. Our work suggests that a heterosubtypic neutralizing antibody response primarily targeting the HA stem region exists in recipients of the 2009 pandemic H1N1 influenza vaccine. The HA stem region contains various conserved neutralizing epitopes with the fusion peptide as an important one. This work may aid in the design of a universal influenza A virus vaccine.

Hu, Weibin [Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China)] [Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China); Chen, Aizhong [Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)] [Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China); Miao, Yi [Shanghai Xuhui Central Hospital, Shanghai 200031 (China)] [Shanghai Xuhui Central Hospital, Shanghai 200031 (China); Xia, Shengli [Center for Disease Control and Prevention of Henan Province, Zhengzhou 450016 (China)] [Center for Disease Control and Prevention of Henan Province, Zhengzhou 450016 (China); Ling, Zhiyang; Xu, Ke; Wang, Tongyan [Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China)] [Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China); Xu, Ying; Cui, Jun; Wu, Hongqiang; Hu, Guiyu; Tian, Lin; Wang, Lingling [Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)] [Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China); Shu, Yuelong [Chinese Center for Disease Control and Prevention, Beijing 102206 (China)] [Chinese Center for Disease Control and Prevention, Beijing 102206 (China); Ma, Xiaowei [Hualan Biological Bacterin Company, Xinxiang 453003 (China)] [Hualan Biological Bacterin Company, Xinxiang 453003 (China); Xu, Bianli; Zhang, Jin [Center for Disease Control and Prevention of Henan Province, Zhengzhou 450016 (China)] [Center for Disease Control and Prevention of Henan Province, Zhengzhou 450016 (China); Lin, Xiaojun, E-mail: linxiaojun@hualan.com [Hualan Biological Bacterin Company, Xinxiang 453003 (China)] [Hualan Biological Bacterin Company, Xinxiang 453003 (China); Bian, Chao, E-mail: cbian@sibs.ac.cn [Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)] [Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China); Sun, Bing, E-mail: bsun@sibs.ac.cn [Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China) [Molecular Virus Unit, Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025 (China); Key Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 (China)

2013-01-20T23:59:59.000Z

354

Overexpression of the human BCL-2 gene product results in growth enhancement of Epstein-Barr virus-immortalized B cells  

SciTech Connect (OSTI)

The biological activity of the human BCL-2 gene product was analyzed in an Epstein-Barr virus (EBV)-infected human lymphoblastoid B-cell line transfected with BCL-2 sequences driven by the simian virus 40 promoter and enhancer. Overproduction of the BCL-2 protein conferred a selective growth advantage to the EBV-infected B cells as compared with control transfectants in low-serum medium and also after seeding at limiting dilution but did not render the cells tumorigenic in athymic nude mice. This growth enhancement was also seen in cells transfected with the BCL-2 gene with its own promoter juxtaposed to the immunoglobulin heavy chain gene enhancer, which represents the translocated form of the BCL-2 gene observed in follicular lymphomas with the t(14;18) translocation. The growth advantage of EBV-infected B cells overproducing the BCL-2 protein is neither due to the enhanced growth factor production nor due to an enhanced sensitivity of the BCL-2 transfectants to interleukins 1 or 6, although both lymphokines are known to stimulate proliferation of EBV-infected B-cell lines. The growth advantage of EBV-infected B-cell lines. The growth advantage of EBV-infected B cells by overproduction of the BCL-2 protein suggests the direct involvement of the BCL-2 gene product in the pathogenesis of follicular lymphoma.

Tsujimoto, Yoshihide (Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA))

1989-03-01T23:59:59.000Z

355

Kinetics of interaction of Cotton Leaf Curl Kokhran Virus-Dabawali (CLCuKV-Dab) coat protein and its mutants with ssDNA  

SciTech Connect (OSTI)

Gemini viral assembly and transport of viral DNA into nucleus for replication, essentially involve DNA-coat protein interactions. The kinetics of interaction of Cotton Leaf Curl Kokhran Virus-Dabawali recombinant coat protein (rCP) with DNA was studied by electrophoretic mobility shift assay (EMSA) and surface plasmon resonance (SPR). The rCP interacted with ssDNA with a K{sub A}, of 2.6 +- 0.29 x 10{sup 8} M{sup -1} in a sequence non-specific manner. The CP has a conserved C2H2 type zinc finger motif composed of residues C68, C72, H81 and H85. Mutation of these residues to alanine resulted in reduced binding to DNA probes. The H85A mutant rCP showed the least binding with approximately 756 fold loss in the association rate and a three order magnitude decrease in the binding affinity as compared to rCP. The CP-DNA interactions via the zinc finger motif could play a crucial role in virus assembly and in nuclear transport.

Priyadarshini, C.G. Poornima [Department of Biochemistry, Indian Institute of Science, Bangalore 560012 (India); Savithri, H.S., E-mail: bchss@biochem.iisc.ernet.i [Department of Biochemistry, Indian Institute of Science, Bangalore 560012 (India)

2009-04-10T23:59:59.000Z

356

adenovirus-vectored vaccine protects: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

under the terms Plotkin, Joshua B. 4 Free Flu Vaccinations protect yourself and your family Environmental Sciences and Ecology Websites Summary: Free Flu Vaccinations protect...

357

Changes to sickness absence reason categories The sickness absence reasons within iTrent have been changed from 18 March  

E-Print Network [OSTI]

strain sprain or injury Cold, cough, Influenza Cold, Cough, Flu - Influenza Cold/Influenza Asthma Asthma problems - (exclude nose & throat problems, asthma, cold, cough, flu) Respiratory Infection Headache

Burton, Geoffrey R.

358

Cheng LL, et al. 2001. Characterization of an endogenous gene expressed in Aedes aegypti using an orally infec-tious recombinant Sindbis virus. 7 pp. Journal of Insect Science, 1:10. Available online: insectscience.org/1.10  

E-Print Network [OSTI]

Cheng LL, et al. 2001. Characterization of an endogenous gene expressed in Aedes aegypti using RNA virus (Togaviridae family) that naturally cycles between mosquitoes and avian hosts (Taylor et al and in vivo and offer great potential for gene characterization (Jiang et al, 1995, Gaines et al., 1996, Higgs

Lowenberger, Carl

359

January 19, 2010 Volume 2, Issue 8  

E-Print Network [OSTI]

/urinary tract infections, bruises, colds, coughs, runny noses, cold sores, ear aches and infections, flu

Jiang, Tiefeng

360

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS 1 High Specificity Binding of Lectins to  

E-Print Network [OSTI]

that affect mankind, including cancer, diabetes, heart diseases, infectious diseases, flu, Alzheimer

Dagenais, Mario

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

The conserved glycine residues in the transmembrane domain of the Semliki Forest virus fusion protein are not required for assembly and fusion  

SciTech Connect (OSTI)

The alphavirus Semliki Forest virus (SFV) infects cells via a low pH-triggered fusion reaction mediated by the viral E1 protein. Both the E1 fusion peptide and transmembrane (TM) domain are essential for membrane fusion, but the functional requirements for the TM domain are poorly understood. Here we explored the role of the five TM domain glycine residues, including the highly conserved glycine pair at E1 residues 415/416. SFV mutants with alanine substitutions for individual or all five glycine residues (5G/A) showed growth kinetics and fusion pH dependence similar to those of wild-type SFV. Mutants with increasing substitution of glycine residues showed an increasingly more stringent requirement for cholesterol during fusion. The 5G/A mutant showed decreased fusion kinetics and extent in fluorescent lipid mixing assays. TM domain glycine residues thus are not required for efficient SFV fusion or assembly but can cause subtle effects on the properties of membrane fusion.

Liao Maofu [Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 (United States); Kielian, Margaret [Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461 (United States)]. E-mail: kielian@aecom.yu.edu

2005-02-05T23:59:59.000Z

362

Mapping regions of Epstein-Barr virus (EBV) glycoprotein B (gB) important for fusion function with gH/gL  

SciTech Connect (OSTI)

Glycoproteins gB and gH/gL are required for entry of Epstein-Barr virus (EBV) into cells, but the role of each glycoprotein and how they function together to mediate fusion is unclear. Analysis of the functional homology of gB from the closely related primate gammaherpesvirus, rhesus lymphocryptovirus (Rh-LCV), showed that EBV gB could not complement Rh gB due to a species-specific dependence between gB and gL. To map domains of gB required for this interaction, we constructed a panel of EBV/Rh gB chimeric proteins. Analysis showed that insertion of Rh gB from residues 456 to 807 restored fusion function of EBV gB with Rh gH/gL, suggesting this region of gB is important for interaction with gH/gL. Split YFP bimolecular complementation (BiFC) provided evidence of an interaction between EBV gB and gH/gL. Together, our results suggest the importance of a gB-gH/gL interaction in EBV-mediated fusion with B cells requiring the region of EBV gB from 456 to 807.

Plate, Aileen E.; Reimer, Jessica J. [Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago IL 60611 (United States); Jardetzky, Theodore S. [Department of Structural Biology, Stanford University School of Medicine, 371 Serra Mall, Stanford, CA 94305 (United States); Longnecker, Richard, E-mail: r-longnecker@northwestern.ed [Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago IL 60611 (United States)

2011-04-25T23:59:59.000Z

363

Different receptors binding to distinct interfaces on herpes simplex virus gD can trigger events leading to cell fusion and viral entry  

SciTech Connect (OSTI)

One of the herpes simplex virus envelope glycoproteins, designated gD, is the principal determinant of cell recognition for viral entry. Other viral glycoproteins, gB, gH and gL, cooperate with gD to mediate the membrane fusion that is required for viral entry and cell fusion. Membrane fusion is triggered by the binding of gD to one of its receptors. These receptors belong to three different classes of cell surface molecules. This review summarizes recent findings on the structure and function of gD. The results presented indicate that gD may assume more than one conformation, one in the absence of receptor, another when gD is bound to the herpesvirus entry mediator, a member of the TNF receptor family, and a third when gD is bound to nectin-1, a cell adhesion molecule in the immunoglobulin superfamily. Finally, information and ideas are presented about a membrane-proximal region of gD that is required for membrane fusion, but not for receptor binding, and that may have a role in activating the fusogenic activity of gB, gH and gL.

Spear, Patricia G. [Department of Microbiology-Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611 (United States)]. E-mail: p-spear@northwestern.edu; Manoj, Sharmila [Department of Microbiology-Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611 (United States); Yoon, Miri [Department of Microbiology-Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611 (United States); Jogger, Cheryl R. [Department of Microbiology-Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611 (United States); Zago, Anna [Department of Microbiology-Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611 (United States); Myscofski, Dawn [Department of Microbiology-Immunology, MC S213, Feinberg School of Medicine, Northwestern University, 320 East Superior Street, Chicago, IL 60611 (United States)

2006-01-05T23:59:59.000Z

364

Investigation of the function of the putative self-association site of Epstein-Barr virus (EBV) glycoprotein 42 (gp42)  

SciTech Connect (OSTI)

The Epstein-Barr virus (EBV) glycoprotein 42 (gp42) is a type II membrane protein essential for entry into B cells but inhibits entry into epithelial cells. X-ray crystallography suggests that gp42 may form dimers when bound to human leukocyte antigen (HLA) class II receptor (Mullen et al., 2002) or multimerize when not bound to HLA class II (Kirschner et al., 2009). We investigated this self-association of gp42 using several different approaches. We generated soluble mutants of gp42 containing mutations within the self-association site and found that these mutants have a defect in fusion. The gp42 mutants bound to gH/gL and HLA class II, but were unable to bind wild-type gp42 or a cleavage mutant of gp42. Using purified gp42, gH/gL, and HLA, we found these proteins associate 1:1:1 by gel filtration suggesting that gp42 dimerization or multimerization does not occur or is a transient event undetectable by our methods.

Rowe, Cynthia L., E-mail: c-rowe@northwestern.edu [Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611 (United States); Matsuura, Hisae, E-mail: hisaem@stanford.edu [Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305 (United States); Interdepartmental Biological Sciences Program, Northwestern University, Evanston, IL 60208 (United States); Jardetzky, Theodore S., E-mail: tjardetz@stanford.edu [Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305 (United States); Longnecker, Richard, E-mail: r-longnecker@northwestern.edu [Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611 (United States)

2011-07-05T23:59:59.000Z

365

HoxD10 gene delivery using adenovirus/adeno-associate hybrid virus inhibits the proliferation and tumorigenicity of GH4 pituitary lactotrope tumor cells  

SciTech Connect (OSTI)

Prolactinoma is one of the most common types of pituitary adenoma. It has been reported that a variety of growth factors and cytokines regulating cell growth and angiogenesis play an important role in the growth of prolactinoma. HoxD10 has been shown to impair endothelial cell migration, block angiogenesis, and maintain a differentiated phenotype of cells. We investigated whether HoxD10 gene delivery could inhibit the growth of prolactinoma. Rat GH4 lactotrope tumor cells were infected with adenovirus/adeno-associated virus (Ad/AAV) hybrid vectors carrying the mouse HoxD10 gene (Hyb-HoxD10) or the {beta}-galactosidase gene (Hyb-Gal). Hyb-HoxD10 expression inhibited GH4 cell proliferation in vitro. The expression of FGF-2 and cyclin D2 was inhibited in GH4 cells infected with Hyb-HoxD10. GH4 cells transduced with Hyb-HoxD10 did not form tumors in nude mice. These results indicate that the delivery of HoxD10 could potentially inhibit the growth of PRL-secreting tumors. This approach may be a useful tool for targeted therapy of prolactinoma and other neoplasms.

Cho, Mi Ae [Division of Endocrinology, Pituitary Tumor Clinic, Research Institute of Endocrinology, Yonsei University College of Medicine, Seoul (Korea, Republic of); Endocrinology, Dong Rae Bong Seng Hospital, Busan (Korea, Republic of); Yashar, Parham [Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States); Department of Neurosurgery, University of Southern California - Keck School of Medicine, Los Angeles, CA (United States); Kim, Suk Kyoung; Noh, Taewoong [Division of Endocrinology, Pituitary Tumor Clinic, Research Institute of Endocrinology, Yonsei University College of Medicine, Seoul (Korea, Republic of); Gillam, Mary P. [Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States); Lee, Eun Jig [Division of Endocrinology, Pituitary Tumor Clinic, Research Institute of Endocrinology, Yonsei University College of Medicine, Seoul (Korea, Republic of); Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States)], E-mail: EJLEE423@yuhs.ac; Jameson, J. Larry [Endocrinology, Metabolism, and Molecular Medicine, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611 (United States)

2008-07-04T23:59:59.000Z

366

Structural Analysis of a Viral Ovarian Tumor Domain Protease from the Crimean-Congo Hemorrhagic Fever Virus in Complex with Covalently Bonded Ubiquitin  

SciTech Connect (OSTI)

Crimean-Congo hemorrhagic fever (CCHF) virus is a tick-borne, negative-sense, single-stranded RNA [ssRNA(-)] nairovirus that produces fever, prostration, and severe hemorrhages in humans. With fatality rates for CCHF ranging up to 70% based on several factors, CCHF is considered a dangerous emerging disease. Originally identified in the former Soviet Union and the Congo, CCHF has rapidly spread across large sections of Europe, Asia, and Africa. Recent reports have identified a viral homologue of the ovarian tumor protease superfamily (vOTU) within its L protein. This protease has subsequently been implicated in downregulation of the type I interferon immune response through cleavage of posttranslational modifying proteins ubiquitin (Ub) and the Ub-like interferon-simulated gene 15 (ISG15). Additionally, homologues of vOTU have been suggested to perform similar roles in the positive-sense, single-stranded RNA [ssRNA(+)] arteriviruses. By utilizing X-ray crystallographic techniques, the structure of vOTU covalently bound to ubiquitin propylamine, a suicide substrate of the enzyme, was elucidated to 1.7 {angstrom}, revealing unique structural elements that define this new subclass of the OTU superfamily. In addition, kinetic studies were carried out with aminomethylcoumarin (AMC) conjugates of monomeric Ub, ISG15, and NEDD8 (neural precursor cell expressed, developmentally downregulated 8) substrates in order to provide quantitative insights into vOTU's preference for Ub and Ub-like substrates.

Capodagli, Glenn C.; McKercher, Marissa A.; Baker, Erica A.; Masters, Emily M.; Brunzelle, Joseph S.; Pegan, Scott D. (Denver); (NWU)

2014-10-02T23:59:59.000Z

367

Mutational analysis of three predicted 5'-proximal stem-loop structures in the genome of tick-borne encephalitis virus indicates different roles in RNA replication and translation  

SciTech Connect (OSTI)

Flavivirus gene expression is modulated by RNA secondary structure elements at the terminal ends of the viral RNA molecule. For tick-borne encephalitis virus (TBEV), four stem-loop (SL) elements have been predicted in the first 180 nucleotides of the viral genome: 5'-SL1, 5'-SL2, 5'-SL3 and 5'-SL4. The last three of these appear to be unique to tick-borne flaviviruses. Here, we report their characterization by mutagenesis in a TBEV luciferase reporter system. By manipulating their thermodynamic properties, we found that an optimal stability of the 5'-SL2 is required for efficient RNA replication. 5'-SL3 formation is also important for viral RNA replication, but although it contains the viral start codon, its formation is dispensable for RNA translation. 5'-SL4 appears to facilitate both RNA translation and replication. Our data suggest that maintenance of the balanced thermodynamic stability of these SL elements is important for temporal regulation of its different functions.

Rouha, Harald; Hoenninger, Verena M.; Thurner, Caroline; Mandl, Christian W., E-mail: christian.mandl@meduniwien.ac.at

2011-08-15T23:59:59.000Z

368

Heat shock factor 1 upregulates transcription of Epstein-Barr Virus nuclear antigen 1 by binding to a heat shock element within the BamHI-Q promoter  

SciTech Connect (OSTI)

Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is essential for maintenance of the episome and establishment of latency. In this study, we observed that heat treatment effectively induced EBNA1 transcription in EBV-transformed B95-8 and human LCL cell lines. Although Cp is considered as the sole promoter used for the expression of EBNA1 transcripts in the lymphoblastoid cell lines, the RT-PCR results showed that the EBNA1 transcripts induced by heat treatment arise from Qp-initiated transcripts. Using bioinformatics, a high affinity and functional heat shock factor 1 (HSF1)-binding element within the - 17/+4 oligonucleotide of the Qp was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Moreover, heat shock and exogenous HSF1 expression induced Qp activity in reporter assays. Further, RNA interference-mediated HSF1 gene silencing attenuated heat-induced EBNA1 expression in B95-8 cells. These results provide evidence that EBNA1 is a new target for the transcription factor HSF1.

Wang, Feng-Wei [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Wu, Xian-Rui [Department of Surgery, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou (China)] [Department of Surgery, Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou (China); Liu, Wen-Ju; Liao, Yi-Ji [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Lin, Sheng [Laboratory of Integrated Biosciences, School of Life Science, Sun Yat-sen University, Guangzhou (China)] [Laboratory of Integrated Biosciences, School of Life Science, Sun Yat-sen University, Guangzhou (China); Zong, Yong-Sheng; Zeng, Mu-Sheng; Zeng, Yi-Xin [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Mai, Shi-Juan, E-mail: maishj@sysucc.org.cn [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China); Xie, Dan, E-mail: xied@mail.sysu.edu.cn [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)] [The State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou (China)

2011-12-20T23:59:59.000Z

369

Structure and Mutagenesis of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Stalk Domain Reveals a Four-Helix Bundle and the Role of the Stalk in Fusion Promotion  

SciTech Connect (OSTI)

Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (hemagglutinin-neuraminidase [HN], H, or G). The multifunctional HN protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin activity) and the receptor-destroying protein (neuraminidase activity), enhances F activity, presumably by lowering the activation energy required for F to mediate fusion of viral and cellular membranes. Before or upon receptor binding by the HN globular head, F is believed to interact with the HN stalk. Unfortunately, until recently none of the receptor binding protein crystal structures have shown electron density for the stalk domain. Parainfluenza virus 5 (PIV5) HN exists as a noncovalent dimer-of-dimers on the surface of cells, linked by a single disulfide bond in the stalk. Here we present the crystal structure of the PIV5-HN stalk domain at a resolution of 2.65 {angstrom}, revealing a four-helix bundle (4HB) with an upper (N-terminal) straight region and a lower (C-terminal) supercoiled part. The hydrophobic core residues are a mix of an 11-mer repeat and a 3- to 4-heptad repeat. To functionally characterize the role of the HN stalk in F interactions and fusion, we designed mutants along the PIV5-HN stalk that are N-glycosylated to physically disrupt F-HN interactions. By extensive study of receptor binding, neuraminidase activity, oligomerization, and fusion-promoting functions of the mutant proteins, we found a correlation between the position of the N-glycosylation mutants on the stalk structure and their neuraminidase activities as well as their abilities to promote fusion.

Bose, Sayantan; Welch, Brett D.; Kors, Christopher A.; Yuan, Ping; Jardetzky, Theodore S.; Lamb, Robert A. (NWU); (Stanford-MED)

2014-10-02T23:59:59.000Z

370

Ca uses o f seaso nal flu c tu atio ns a nd abu n-da nce in shrimp po pul atio ns in the estuaries  

E-Print Network [OSTI]

Qicai fdC(pr..... and conduct a brief sune) 01 the bait shnmfl fisher) In the Lower Laguna \\I"dre In prep

371

The Office of Human Resources is pleased to announce that free seasonal flu vaccines will again be available for active, benefits-  

E-Print Network [OSTI]

of germs by covering your coughs and sneezes, washing your hands, and staying home if you are sick. · Take

Subramanian, Venkat

372

arXiv:0904.0799v1[physics.flu-dyn]5Apr2009 Bistability and hysteresis of dipolar dynamos generated by turbu-  

E-Print Network [OSTI]

and it is believed to be responsible for the mag- netic fields of cosmic objects including the sun and most planets distribution TS = T0 - d2 r2 /2 and a gravity field in the form g = -dr, where rd is the length of the posi- tion vector with respect to the center of the sphere. This form of temperature profile alludes

Schmidt, Matthias

373

arXiv:0806.3050v1[physics.flu-dyn]18Jun2008 Rayleigh-Plateau instability causes the crown splash  

E-Print Network [OSTI]

, cooling, and combustion. In the crown splash parameter regime, the splash pattern is highly regular. We the air-sea interface [1], cooling [2], coatings [3], and combus- tion [4]. The spatial pattern and size in Edgerton's iconic photograph Milk Coronet [9], and show that number of secondary droplets is governed

Eggers, Jens

374

arXiv:0902.3733v1[physics.flu-dyn]21Feb2009 APS/123-QED Rotating electrohydrodynamic flow in a suspended liquid film  

E-Print Network [OSTI]

film motor, emphasizing that it represents a new type of engine. They also proposed that it could be ex of the liquid film motor [1, 2]. PACS numbers: 47.32.Ef, 68.15.+e, 47.57.jd Keywords: electrohydrodynamic flow), but it can not exist for very thin films. An important general result of our paper is the demonstration

375

Nigon VM, Samarut J, Verdier G, Flamant F, Benchaibi M, Poncet D, Savatier P, Chambonnet F, Thoraval P, Faure C, Lan-glois P, 1986. Use of avian erythroblastosis virus to produce vectors for gene transfer in poultry. 7th European Poultry  

E-Print Network [OSTI]

transfer in poultry. 7th European Poultry Conference. Paris, 38-44 PROBL?MES LI?S A L'UTILISATION DE erythroblastosis virus to produce vectors for gene transfer in poultry. 7th European Poultry Conference, M Larbier vectors for gene transfer in poultry. 7th European Poultry Conference, M Larbier Ed, Paris, p 38

Paris-Sud XI, Université de

376

Airport baggage scanning technology makes flying safer for Americans  

SciTech Connect (OSTI)

Each time you step on a commercial flight, you can feel safer because of a researcher you've probably never heard of. His name is Harry Martz. He's a veteran scientist at the Lawrence Livermore National Laboratory (LLNL) who wakes up every day thinking how his research can advance X-ray imaging technology to thwart the next terrorist attack. "My job is to improve national security," Martz said. "That's why my research team exists. We have to outsmart the terrorists. It's a constant battle."

Martz, Harry

2013-08-28T23:59:59.000Z

377

Reemergence of Dengue Virus Type  

E-Print Network [OSTI]

of the French West Indies in northern South America between Suriname and Brazil. DENV is endemic in French

Paris-Sud XI, Université de

378

Evolutionary Genomics of Prokaryotic Viruses.  

E-Print Network [OSTI]

??Evolutionary history of biological entities is recorded within their nucleic acid sequences and can (sometimes) be deciphered by thorough genomic analysis. In this study we (more)

Krupovic, Mart

2010-01-01T23:59:59.000Z

379

Tanning bed use, deviance regulation theory, and source factors  

E-Print Network [OSTI]

conditions were successful. If participants thought their peers were receiving flu shots, messages which emphasized the negative attribute of 13 irresponsibility for people who did not receive a flu shot were effective at changing individuals...

Head, Katharine J.

2009-05-15T23:59:59.000Z

380

Dr. Jianlin Cheng Department of Computer Science  

E-Print Network [OSTI]

;Applications of a posterior belief #12;An Example Flu Fever High Temperature Cough #12;An Example ­ Combining Evidences Flu Fever High Temperature Cough #12;Inferential query 3: most probable assignment #12;Inferential

Cheng, Jianlin Jack

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
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381

E-Print Network 3.0 - avian development facility Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

to be a control method... of Avian Pathologists and The American College of Poultry Veterinarians VSE 05-01 What is "Asian Bird Flu... "? "Asian Bird Flu" is the name...

382

Healthy Environment, Healthy Children, and Healthy Future: You Take My Breath Away  

E-Print Network [OSTI]

for asthma, colds, flu, coughs, allergies, sinusitus.et al. (1996). Recurrent cough in childhood and its relation

Johnson, William Ted

2001-01-01T23:59:59.000Z

383

PHYSICS, COMPUTER SCIENCE AND MATHEMATICS DIVISION. ANNUAL REPORT  

E-Print Network [OSTI]

October 1977. AND OF flU The HEMP User's Manual Lawrenceprocess of implementation of the new HEMP was substantially

Birge, Robert W.

2011-01-01T23:59:59.000Z

384

April 8, 2010 MSU Community Music School launches donation drive for Gateway  

E-Print Network [OSTI]

/children Children's books Medications Pepto-Bismol® Anti Diarrhea Cold Medicine Flu Medicine Cough Syrup Cough

Liu, Taosheng

385

Molecular studies of avian leukosis virus  

E-Print Network [OSTI]

below. The locations of the open reading frames gag, pol, and env are shown. Reverse transcription of the RNA results in rearrangemnet of the termini of the genome, resulting in the structures of the LTRs (long terminal repeats) as indicated. The 5...? end of the genomic RNA begins with the ?r? (for repeat) and ?u5? (for unique 5? region) segments, followed by the viral genes gag, pol, and env. The 3? end of this sequence is terminated by the u3 (for unique 3? region) and r (identical to the 5...

Mozisek, Blayne Myron

2007-04-25T23:59:59.000Z

386

Neurotropism of Theiler's murine encephalomyelitis virus  

E-Print Network [OSTI]

Figure 2. H&E photomicrographs of cross sections of spinal cord sections in preliminary experiment. Panels A, B, C, E, & F were taken at 10X. Panels D were taken at 63X. (A) NIF mouse. (B,C) IF mouse section that shows ventral lateral meningitis...

Villarreal, Dorissa

2005-11-01T23:59:59.000Z

387

Capsid and Infectivity in Virus Detection  

E-Print Network [OSTI]

irradiation is often used to decontaminate water, waste- water, and surfaces, although it cannot penetrate foods

Cliver, Dean O.

2009-01-01T23:59:59.000Z

388

Selecting a path against Hepatitis C Virus  

E-Print Network [OSTI]

that both TACR1 and TACR3 inhibitors significantly inhibit HCV infection. These results point to the potential for TACR1 antagonists in treating patients infected with both HCV and HIV....

Simeon, Rudo Lyndon

2013-12-12T23:59:59.000Z

389

Genetic resistance to Infectious Bronchitis Virus infection  

E-Print Network [OSTI]

(N-line, PDRC) or susceptible (S-strain, P-line), did not follov the same pattern of genetic immunity when challenged with RSV. The S-strain had weak RSV induced tumor regression, while PDRC line was a strong regressor. This suggests a correlation... complete protection in the chicken. Thus far, limited studies have used these inbred lines to examine challenge with IBV, and no IBV research has been conducted on the lines used. This study identified genetic differences in susceptibility to IBV...

Dzielawa, Jennifer Ann

2001-01-01T23:59:59.000Z

390

Bioterrorism Preparedness: Smallpox and Related Pox Viruses  

E-Print Network [OSTI]

, Indonesia. In recent years, concerns over dis- eases such as Ebola have drastically restricted the importation of mon- keys. However, large populations exist in research facilities and zoos across the United States. Conse- quently, human and sub...

Lawhorn, D. Bruce

2002-04-24T23:59:59.000Z

391

E-Print Network 3.0 - atypical bacteria accompanying Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Swine Flu and Common Infections to Prepare For Summary: Other common causes - Whooping cough (pertussis) - Atypical bacteria Symptoms - Severe cough - Runny... :...

392

E-Print Network 3.0 - antibiotics antifungal Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

(Foot) Antiparasitic treatments Antiseptics & wound cleansers Anti... Contraceptives Cough, cold & flu Denture pain relief Digestive aids Ear care Eye care Feminine antifungal &...

393

E-Print Network 3.0 - affects blood pressure Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

obesity swine flu high blood pressure parkinson's stress depression muscle... stiffness heart attack asthma low blood pressure alzheimer's cancer diabetes kidney failure...

394

E-Print Network 3.0 - anhydride-induced occupational asthma Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

37 varicose veins smoking obesity swine flu high blood pressure Summary: stiffness heart attack asthma low blood pressure alzheimer's cancer diabetes kidney failure...

395

E-Print Network 3.0 - affect blood pressure Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

obesity swine flu high blood pressure parkinson's stress depression muscle... stiffness heart attack asthma low blood pressure alzheimer's cancer diabetes kidney failure...

396

E-Print Network 3.0 - agricultural anthrax disinfection Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Pennsylvania State University Collection: Biology and Medicine 6 NEWSPAPER SEARCH Enter Keyword(s) FIND A BUSINESS Enter Category Scientists work to build better flu trap...

397

Richard Merrill: Writer and Educator  

E-Print Network [OSTI]

so-called stomach flu is food poisoning, and if you eat outyoure going to get food poisoning twice as much as the

Farmer, Ellen

2010-01-01T23:59:59.000Z

398

Activated Carbon Injection  

SciTech Connect (OSTI)

History of the Clean Air Act and how the injection of carbon into a coal power plant's flu smoke can reduce the amount of mercury in the smoke.

None

2014-07-16T23:59:59.000Z

399

November 30, 2010 OSHA Mandate for Tdap Vaccine  

E-Print Network [OSTI]

Tetanus (Lockjaw), Diptheria and Pertussis (Whooping Cough). Employee Health's roving flu clinic cart present as a mild, but chronic infectious cough. It's when an infected adult unsuspectingly infects

Leistikow, Bruce N.

400

E-Print Network 3.0 - ambient carbon monoxide Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

FACT SHEET Carbon monoxide (CO), known as the Invisible Killer... , propane, oil, wood, coal, and gasoline. Exposure to carbon monoxide can cause flu-like symptoms and can... .)...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Activated Carbon Injection  

ScienceCinema (OSTI)

History of the Clean Air Act and how the injection of carbon into a coal power plant's flu smoke can reduce the amount of mercury in the smoke.

None

2014-07-22T23:59:59.000Z

402

Cross protection among some strains of sugarcane mosaic virus and maize dwarf mosaic virus (*)  

E-Print Network [OSTI]

), were maintained on young sweet corn seedlings. #12;All inoculations, using carborundum as an aid, were

Boyer, Edmond

403

H1N1 Influenza: What You Need to Know if You Live in Residence Welcome to residence at the University of Toronto. We're delighted that you'll be living  

E-Print Network [OSTI]

flu have been similar to the symptoms of seasonal flu: a temperature of more than 38°C, cough, sore your hands often. · Cough or sneeze into your sleeve or a tissue. · Stay at home or in your residence-the-counter medication to reduce aches and fever (e.g., acetaminophen or ibuprofen) · Cough medicine and throat lozenges

Boonstra, Rudy

404

H1N1 Influenza: Protecting Yourself and Others Welcome to the University of Toronto! We're delighted that you're here,  

E-Print Network [OSTI]

, coughing/sore throat, fatigue, nausea/vomiting and diarrhea. The University is prepared to manage H1N1 flu of flu: · Wash and/or sanitize your hands frequently. · Cough and sneeze into a tissue or your sleeve

Boonstra, Rudy

405

&('0)21436587@9 ACBED6BEDGFH5I36PRQTSU'036VW5XFH1`Yacb0'edea fhgi0prqtstuvgwxsyrHCqpwprwywIstgXgw0gIdegfheCpujiIqkXgEdmlonpXq2rs  

E-Print Network [OSTI]

Coughing Fever Headache Working In Factory Smoking Stressful Lifestyle High Fat Diet Flu Bronchitis Lung Cancer Heart Disease Chest Pain Coughing Fever Headache ï ú ù ï©ù Working In Factory Smoking Stressful Lifestyle High Fat Diet Flu Bronchitis Lung Cancer Heart Disease Chest Pain Coughing Fever Headache Working

Tenenbaum, Josh

406

A comparison of assay techniques of corn and sorghum viruses  

E-Print Network [OSTI]

. Colowick and N. O. Kaplan, eds. Methods in enzymology. Vol. I. Academic Press, New York. 31. NAKANE, P. K. and A. KAWAOI. 1974. Peroxidase- labelled antibody ? a new method of conjugation. J. Histochem. Cytochem. 22:1084-1091. 32. NIKOLIC, V. and D..., was also studied. Immunosorbent plates were coated rv with gamma globulin specific for NDMV. Two enzymes, horse- radish peroxidase and alkaline phosphatase, were each conjugated to NDMV specific antisera. Each worked well being as sensitive as 50 pg...

Huebner, Rae Allison

1978-01-01T23:59:59.000Z

407

TUMOUR IMMUNOLOGY Keeping virus-driven lymphomas in check  

E-Print Network [OSTI]

,acombinationofthetumour-targetingantibody trastuzumab(whichisspecificforhumanepidermalgrowthfactor receptor 2(HER2))andanaturalkiller(NK)cell vasculature. Leukocyte adhesion to endothelial cells lining the tumour-associated vasculature is impaired and cleared by the immune system, but it can persist in some cells for life. Under conditions

Cai, Long

408

abelson virus transformation: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that allows to specify binary relations on graphs Drewes, Frank 27 Constructors transformers Computer Technologies and Information Sciences Websites Summary: Proc. AGTIVE'99,...

409

al virus del: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Information Sciences Websites Summary: Roma. Alan Turing's legacy | L'eredit di Alan Turing Roma 12 e 13 Ottobre 2012 Accoglienza dei Numerico Giuseppe Longo h. 16.45-17.30...

410

acute friend virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the great majority the feasibility of our attack. The friend injection attack enables a stealth infiltra- tion of social networks Boyer, Edmond 209 Immunofluorescent studies of...

411

ancient virus world: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

religion in the ancient Mediterranean permeated aspects of everyday life, including seafaring. Besides cargo, ships transported mariners' religious beliefs from port to port,...

412

ATTEMPTS TOISOLATE BOVINE LEUKEMIA AND BOVINE SYNCYTIAL VIRUSES FROM BLOOD,  

E-Print Network [OSTI]

by embryo transfer (Mitchell and Betteridge, 1977 ; Betteridge, 1978 ; Bowen, 1979 ; Waters, 1981), which indicates that the probability of congenital transmission varies from one disease to ano- ther (reviewed

Paris-Sud XI, Université de

413

Virus-Enabled Silicon Anode for Lithium-Ion Batteries  

E-Print Network [OSTI]

ratio nanofeatured surfaces with electroless deposition to produce an integrated nickel current coating in electroless plating solutions due to strong covalent-like interactions between the thiol groups particle) for the synthesis of nickel and cobalt nanowires. These struc- tures self-assemble vertically

Rubloff, Gary W.

414

Feline Urological Syndrome: is a virus responsible for the disease?  

E-Print Network [OSTI]

-associated her pesvir us (CAHV) could not be r escued fr om any of the tissues or fluids assayed. Nor did any of the feline ser a tested contain vir us-neutr alizing antibody to CAHV or to several other her pesviruses that cats may commonly encounter... of triple ammonium magnesium phosphate (struvite) cr ystals (10) . Stones may contr ibute to the accumulation of cellular debr is fr om the bladder, forming a urethral plug. Large stone may become lodged in the urethra, resulting in obstruction...

Swansn, Cynthia Louise

1984-01-01T23:59:59.000Z

415

Sophos Anti-Virus for Windows, version 7 user manual  

E-Print Network [OSTI]

.................................................................................................................................25 11 Logging as part of your company policy devices that are blocked as part of your company policy on your computer and PUAs from running on your computer clean adware and PUAs from your computer keep a log of its

416

Phylogenetic assessment of filoviruses: how many lineages of Marburg virus?  

E-Print Network [OSTI]

for the Filoviridae: using coalescent simulations, we ascertain that two Marburg isolates (termed the RAVN strain) represent a quite-distinct lineage that should be considered in studies of biogeography and host associations, and may merit recognition at the level...

Peterson, A. Townsend; Holder, Mark T.

2012-07-01T23:59:59.000Z

417

alimentos por virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mungu-aAgust-n Lpez Mungu-a descubrir-amos Arroyo Rodr-guez, V-ctor 8 Ciencia y Tecnologa de Alimentos Environmental Sciences and Ecology Websites Summary: 1 1 4 7...

418

aids virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Tax applies to the sale of manufacturing aids such as dies, patterns, jigs and tooling used in the manufacturing process notwithstanding the fact that the property used in...

419

Studies on the nucleocapsid protein of infectious bronchitis virus  

E-Print Network [OSTI]

studies that I have performed. vii Thanks to Dr. Paul Gershon, Institute of Biosciences and Technology, Houston, for the use of their Mass Spectrometry facility and for his help with the analysis of results. Thanks to Dr. Duncan MacKenzie, Dr. Mark Zoran... and absence of a serine- kinase inhibitor U0126?????????????.?????.. 56 2.3 Viral titers from IBV-infected Vero cells in the presence and absence of serine-kinase inhibitor U0126???????????????. 56 2.4 Comparison of the mass:charge (m/z) ratios of predicted...

Jayaram, Jyothi

2005-08-29T23:59:59.000Z

420

argentinas del virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

21 22 23 24 25 Next Page Last Page Topic Index 1 Multiplicador del gasto pblico en Argentina. Open Access Theses and Dissertations Summary: ??Este trabajo proporciona por...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

On the Spread of Viruses on the Internet Noam Berger  

E-Print Network [OSTI]

Saberi Abstract We analyze the contact process on random graphs generated according to the preferen- tial}@microsoft.com. Georgia Institute of Technology, Atlanta, GA. E-mail: saberi@cc.gatech.edu. 1 #12;In our context

Saberi, Amin

422

On the Spread of Viruses on the Internet Noam Berger  

E-Print Network [OSTI]

Saberi Abstract We analyze the contact process on random graphs generated according to the preferen- tial of Technology, Atlanta, GA. E-mail: saberi@cc.gatech.edu. 1 #12;status of its neighbors. A healthy vertex

Chaudhuri, Surajit

423

affects virus transmission: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Plants Websites Summary: ) establishes a framework for markets based on locational marginal pricing (LMP). The NOPR envisions a critical incentives. G iven the shortcomings of...

424

Immunogenic compositions comprising human immunodeficiency virus (HIV) mosaic Nef proteins  

DOE Patents [OSTI]

The present invention relates to mosaic clade M HIV-1 Nef polypeptides and to compositions comprising same. The polypeptides of the invention are suitable for use in inducing an immune response to HIV-1 in a human.

Korber, Bette T. (Los Alamos, NM); Perkins, Simon (Los Alamos, NM); Bhattacharya, Tanmoy (Los Alamos, NM); Fischer, William M. (Los Alamos, NM); Theiler, James (Los Alamos, NM); Letvin, Norman (Boston, MA); Haynes, Barton F. (Durham, NC); Hahn, Beatrice H. (Birmingham, AL); Yusim, Karina (Los Alamos, NM); Kuiken, Carla (Los Alamos, NM)

2012-02-21T23:59:59.000Z

425

Human Immunodeficiency Virus gag and protease: partners in resistance  

E-Print Network [OSTI]

% of virologicalrebounders in an analysis of the combined POWER 1, 2 and 3 trials that evaluated virological response to DRV/r plus optimized background therapy in PI-experienced patients [66]. Mutation S373Q (codon 373 is highly polymorphic, 36% variability in subtype B... , with the exception of tipranavir, are competitive peptidomimetic inhibitors, mimicking the natural substrate of the viral PR. The peptidomimetic inhibitors contain a hydro- xyethylene core which prohibits cleavage by the viral PR [18-25]. Instead of a hydroxyethylene...

Fun, Axel; Wensing, Annemarie MJ; Verheyen, Jens; Nijhuis, Monique

2012-08-06T23:59:59.000Z

426

Viruses: incredible nanomachines. New advances with filamentous phages  

E-Print Network [OSTI]

) resonance energy transfer gp8 Gene 8 product; the protein coded by viral gene number 8 (gp3, gp5, gp6, gp7. The technique of phage display has provided powerful methodology for identification and optimisation of ligands

Hemminga, Marcus A.

427

Integrated Nanosystems Templated by Self-assembled Virus Capsids  

E-Print Network [OSTI]

Water (ddH 2 O) used in biological procedures or as reaction solvents was deionized using a NANOpure purification system (Water (ddH 2 O) used in biological procedures or as reaction solvents was deionized using a NANOpure purification system (Water (ddH 2 O) used in biological procedures or as reaction solvents was deionized using a NANOpure purification system (

Stephanopoulos, Nicholas

2010-01-01T23:59:59.000Z

428

What college students should know about Hepatitis B Virus (HBV)  

E-Print Network [OSTI]

. Hepatitis B vaccine is the best protection against HBV and is recommended by leading medical and public to get the vaccine is before arriving on campus in the fall.The vaccine is available from your family recover from the infection, some may develop lifelong infection that can lead to cirrhosis (scarring

Wisconsin at Madison, University of

429

ANALYSIS OF HEPATITIS C VIRUS INFECTION MODELS WITH HEPATOCYTE HOMEOSTASIS  

E-Print Network [OSTI]

ALAMOS NATIONAL LABORATORY LOS ALAMOS, NM 87545 Abstract. Recently, we developed a model for hepatitis C Park PA 16802, ¶Current address: Department of Medicine, University of Illinois at Chicago, Chicago, IL

Reluga, Tim

430

Bifunctional polymeric inhibitors of human influenza A viruses  

E-Print Network [OSTI]

Purpose. New antiviral agents were prepared by attaching derivatives of sialic acid (1) and of the drug zanamivir (2) to poly(isobutylene-alt-maleic anhydride) (poly-(1 + 2)) or by mixing poly-1 and poly-2, followed by ...

Haldar, Jayanta

431

Real-Time Virus Trapping and Fluorescent Imaging in Microfluidic  

E-Print Network [OSTI]

particles; however, this method requires the samples to be dried, fixed, coated, and laboriously prepared time, the images were collected with a computer running a video image capture

Bashir, Rashid

432

ankara mva virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

INTERNATIONAL AEROSPACE CONFERENCE AIAC-2009-034 17-19 August 2009 -METU, Ankara TURKEY Engineering Websites Summary: 5. ANKARA INTERNATIONAL AEROSPACE CONFERENCE...

433

Ecology, diversity and comparative genomics of oceanic cyanobacterial viruses  

E-Print Network [OSTI]

The marine cyanobacteria Prochlorococcus and Synechococcus are numerically dominant primary producers in the oceans. Each genera consists of multiple physiologically and genetically distinct groups (termed "ecotypes" in ...

Sullivan, Matthew Brian, 1975-

2004-01-01T23:59:59.000Z

434

adn del virus: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

riego (more) Calgua de Len, Byron Tomas 2013-01-01 27 Prdiction du grade d'un cancer du sein par la dcouverte de motifs squentiels contextuels dans des puces ADN...

435

Dihydropyridines Inhibit Translation and Early Replication of Hepatitis C Virus  

E-Print Network [OSTI]

of action will be necessary to compliment our current repertoire of anti-HCV therapies. Previously our lab identified three dihydropyridines (DHP) as potent HCV replication inhibitors. We investigated and characterized the anti-HCV properties of nine...

Klemashevich, Cory

2013-05-09T23:59:59.000Z

436

Serodiagnosis of avian infectious bronchitis virus using recombinant nucleocapsid protein  

E-Print Network [OSTI]

A diagnostic immunoaassay using recombinant nucleocapsid protein to detect antibodies to I13V was designed. The nucleocapsid protein was expressed from an expression plasmid, Qiagen pQE8, in E. coli as a fusion protein that was purified on NI...

Ndifuna, Abdul-El-Nuru

1996-01-01T23:59:59.000Z

437

HIV virus spread and evolution studied through computer modeling  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-SeriesFlickr FlickrGuided Self-Assembly of GoldHAWCHIGS flux

438

Virus-based Piezoelectric Energy Generation - Energy Innovation Portal  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism inS-4500IIVasudha PatriPhotoelectronVideosVirginiaInformationEnergy

439

Interactions of ciliates with cells and viruses of fish.  

E-Print Network [OSTI]

??This thesis develops and utilizes in vitro approaches to study ciliate/fish interactions. The thesis is divided into six chapters. Chapter one reviews the literature on (more)

Pinheiro, Marcel D.O.

2013-01-01T23:59:59.000Z

440

Mosaic protein and nucleic acid vaccines against hepatitis C virus  

DOE Patents [OSTI]

The invention relates to immunogenic compositions useful as HCV vaccines. Provided are HCV mosaic polypeptide and nucleic acid compositions which provide higher levels of T-cell epitope coverage while minimizing the occurrence of unnatural and rare epitopes compared to natural HCV polypeptides and consensus HCV sequences.

Yusim, Karina; Korber, Bette T. M.; Kuiken, Carla L.; Fischer, William M.

2013-06-11T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Studies of the restriction of Equine Infectious Anemia Virus  

E-Print Network [OSTI]

different cell types , Page 66 74 INTRODUCTION One of the most economically important diseases of horses is equine 1nfectious anemia (EIA). First described by Lignee in 1843 [23], EIA is a viral disease confined to members of the family Equ1dae... of EIA by agar-gel 1mmunod1ffusion tests on the animals' sera before being allowed to race wi thin those states. Improvements 1n the ability to detect infected animals have helped decrease the overall reported 1ncidence of the d1sease through...

Geyer, David Scott

1981-01-01T23:59:59.000Z

442

Identification and evaluation of an isolate of sugarcane mosaic virus  

E-Print Network [OSTI]

(KPB), pH 7. 0, and containing 0. 5/ activated charcoal and 14 carborundum, between fingers. Mortar and pestle and Waring blender was used for grinding tissue. When liquid nitrogen was employed, KPB and charcoal were added after pulverizing... and uninoculated half- rows). Inoculum Rio Hondo isolate and SCMV-H were increased on Trudan-5. These plants were harvested about one month after inoculation and homogenized in a Haring blender with 0. 1 M KPB, pH 7. 0 (1:4 w/v). Carborundum to 1% (w/v) and 2...

Giorda de Messina, Laura Maria

1983-01-01T23:59:59.000Z

443

The identification and distribution of viruses infecting cucurbits in Texas  

E-Print Network [OSTI]

stage, before the first true leaves appeared. Cotyledons were dusted with 300 mesh carborundum followed by rubbing with buffered infectious plant sap using 0. 05 M potassium phosphate buffer pH 7. 0. Symptoms normally appeared with 12-14 days...

Harveson, Robert M

1989-01-01T23:59:59.000Z

444

Immobilization and One-Dimensional Arrangement of Virus Capsids with  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-SeriesFlickr FlickrGuidedCH2MLLCBasicsScienceRadiation

445

Multidisciplinary team aids understanding of Hepatitis C virus and possible  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment SurfacesResource ProgramModification andinterface1JUN 2 5coreStöhrEMSLLaCrO3.

446

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed Newcatalyst phasesData FilesShape, Density, andaging effectsOfficePortal

447

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed Newcatalyst phasesData FilesShape, Density, andaging effectsOfficePortalDesigner

448

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed Newcatalyst phasesData FilesShape, Density, andaging

449

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct: CrudeOffice ofINLNuclear262About

450

Designer Proteins Target Epstein-Barr-Virus-Associated Cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct: CrudeOffice ofINLNuclear262AboutDesigner Proteins Target

451

ALS Capabilities Reveal Multiple Functions of Ebola Virus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May Jun Jul(Summary)morphinanInformation Desert SouthwestTechnologies |November 2011Astudies smart sensors andScienceALSALSALSALSALS

452

This is a paper model of the MS2 virus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2 andThe1A: Handling of4,3, 20114,0,24,This is Y-12

453

Structural Rearrangement in Ebola Virus Protein VP40 Creates Multiple  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBiSiteNeutronStrategicOur Mission Our Mission The SSRLFilaments

454

ALS Capabilities Reveal Multiple Functions of Ebola Virus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLasDelivered‰PNGExperience4AJ01) (See95TI07)Operations During theALS CapabilitiesALS

455

ALS Capabilities Reveal Multiple Functions of Ebola Virus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLasDelivered‰PNGExperience4AJ01) (See95TI07)Operations During theALS CapabilitiesALSALS

456

ALS Capabilities Reveal Multiple Functions of Ebola Virus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLasDelivered‰PNGExperience4AJ01) (See95TI07)Operations During theALS CapabilitiesALSALSALS

457

ALS Capabilities Reveal Multiple Functions of Ebola Virus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLasDelivered‰PNGExperience4AJ01) (See95TI07)Operations During theALS CapabilitiesALSALSALSALS

458

Mechanisms of virus removal during transport in unsaturated porous media  

E-Print Network [OSTI]

) has proposed the Ground Water Rule, which requires groundwater systems to conduct a hydro- geologic micro- organisms (U.S. EPA, National primary drinking water regulations: Ground Water Rule, proposed to the solid surfaces. Electrostatic and hydro

Flury, Markus

459

E-Print Network 3.0 - anti-motion sickness therapy Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Therapy Dalhousie University If I am sick with flu-like symptoms (i.e., fever andor cough... School of Occupational Therapy Room 215, 5869 University Avenue Halifax, NS...

460

E-Print Network 3.0 - acute gastrointestinal illness Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Flu typically hits in Michigan December-March. Acute symptoms... last 3-4 days, and cough may linger for 3 weeks. Gastrointestinal symptoms are rare. Symptoms include Source:...

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

STATEMENT OF CONSIDERATIONS REQUEST BY AIR PRODUCTS AND CHEMICALS...  

Broader source: Energy.gov (indexed) [DOE]

-- - , 1 .3 t fLU4 ' I 04 - -t - rt . .5 * * STATEMENT OF CONSIDERATIONS REQUEST BY AIR PRODUCTS AND CHEMICALS, INC. FOR AN ADVANCE WAIVER OF PATENT RIGHTS UNDER DOE...

462

CLICK HERE TO PRINT CLOSE WINDOW July 03, 2005  

E-Print Network [OSTI]

tropical commodities such as cotton, tea, coffee, sugar and bananas, and a few mineral resources, precious of the global avian-flu pandemic. Experts had warned of the threat for decades, but to no avail. When it struck

463

November 2002 Vol. 6 No. 2 This is a publication for the staff of The University of Texas at Dallas  

E-Print Network [OSTI]

In This Issue: Flu Shots Campus Development Did You Know? Fall Forum Report President's Message Calendar Insert and stand 18-22 inches high. Colors available: Red, white, pink and marble Cost: $10.00 Purchase deadline

O'Toole, Alice J.

464

Estimating network structure and propagation dynamics for an infectious disease : towards effective vaccine allocation  

E-Print Network [OSTI]

In the event of a pandemic influenza outbreak, such as the 2009-2010 H1N1 "Swine Flu" episode, it is crucial to effectively allocate limited resources in order to minimize the casualties. Design of effective resource ...

Kim, Louis Y. (Louis Yongchul)

2014-01-01T23:59:59.000Z

465

VoxMeDALTHE VOICE OF DALHOUSIE MEDICAL ALUMNI S P R I N G 2 0 1 0  

E-Print Network [OSTI]

PRESIDENT'S MESSAGE F or the DMAA, 2010 brings a sense of renewed energy as we reflect on the past year. Our Swine flu and lessons of Spanish influenza Mailed under Canada Post Publications Mail Agreement

Brownstone, Rob

466

A BRIEF REVIEW ON TWO PANDEMIC ZOONOSES  

E-Print Network [OSTI]

immune system (= resistance to innate and adaptive immune mechanisms performed by the infected host years. The recent occurrence of the potential pandemic H5N1 avian flu, severe acute respiratory syndrome

Bech, Claus

467

Acne treatments (over-the-counter) Acupuncture  

E-Print Network [OSTI]

-the- counter) Corneal keratotomy Cough drops & sore throat lozenges (over-the-counter) Cough syrup (over & flu prevention Over-the-counter cough drops & sore throat lozenges Over-the-counter cough syrup Over

Bordenstein, Seth

468

10 Health Tips for Student Success Don't Wait Until You're Sick to Take Care of Yourself!  

E-Print Network [OSTI]

in college can really set you back. Avoid the fever, aches, fatigue and cough associated with the flu ILLNESS AND INJURIES. Having a thermometer, Tylenol, a decongestant and cough syrup will help you to take

469

E-Print Network 3.0 - avian field guide Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Sciences and Ecology 5 APandemic Upon Us Researchers battle avian flu threats to poultry industry and humans. Summary: - tion,"Wyvill says."Will this sensor work easily in...

470

Texas A&M AgriLife Extension Service 270 Master Wellness Volunteer Program Agent Manual Health Evaluation  

E-Print Network [OSTI]

consumption 6. Which of the following is an example of a chronic disease? a. heartburn b. diabetes c. flu d regular check-ups to help diagnosis diabetes, high blood pressure, high cholesterol at early stages d. all

471

THE THIRTEENTH AMENDMENT IN NATIONAL EMERGENCIES 1 COMPULSORY LABOR IN A NATIONAL EMERGENCY  

E-Print Network [OSTI]

officers and health care workers abandoned their jobs during Hurricane Katrina. Postal clerks worked against their will without masks in facilities with anthrax. A report by Congress worries that avian flu

Gilbert, Matthew

472

Infectious Disease Updates To minimize the risk of any infectious disease, practice these daily preventive  

E-Print Network [OSTI]

these daily preventive measures: · Cover your nose and mouth with a tissue when you cough or sneeze. Throw seconds), especially after you cough or sneeze. Alcohol-based hand cleaners are an alternative://www.sjsu.edu/studenthealth/cold_flu/index.html http://www.cdph.ca.gov/healthinfo/discond/pages/influenza(flu).aspx 2) Pertussis (Whooping Cough) http://www.sjsu.edu/studenthealth/docs/whooping_cough

Su, Xiao

473

The Retrocausal Nature of Quantum Measurement Revealed by Partial and Weak Measurements  

SciTech Connect (OSTI)

Quantum measurement is sometimes more effective when its result is not definite. Partial measurement turns the initial superposition not into a certain state but to a greater probability for it, enabling probing the quantum state in cases where complete measurement makes the noncommuting variables inaccessible. It also enables full quantum erasure that, unlike prevailing method, can be carried out even on recorded results. Aharonov's weak measurement is another method of imprecisely measuring quantum variables, outsmarting the uncertainty principle in even subtler ways. Happily, the two methods complement and corroborate one another in several interesting ways. We gedankenly apply these measurements to the EPR case. A pair of entangled particles undergoes more than one pair of partial and weak measurements, which, unlike complete measurements, leave them partially correlated. Their erasure is then shown to be as nonlocal as measurement itself. Surprisingly, the temporal relations between such measurements in the EPR setting do not follow the temporal sequence perceived by an external observer. For each particle, the measurements performed on the other operate as if they occurred (with signs reversed) in its own past, and in reversed order. This fully accords with Cramer's transactional interpretation and Aharonov's two state-vector formalism.

Elitzur, Avshalom C. [Iyar, Israeli Institute for Advanced Research, Rehovot (Israel); Cohen, Eliahu [School of Physics and Astronomy, Tel-Aviv University, Tel-Aviv 69978 (Israel)

2011-11-29T23:59:59.000Z

474

PUTATIVE GENE PROMOTER SEQUENCES IN THE CHLORELLA Lisa A. Fitzgeralda,#, Philip T. Bouchera, Giane Yanai-Balsera, Karsten Suhreb,c, Michael V.  

E-Print Network [OSTI]

PUTATIVE GENE PROMOTER SEQUENCES IN THE CHLORELLA VIRUSES Lisa A. Fitzgeralda,#, Philip T. Bouchera expressed early in virus replication. Keywords Chlorella viruses; Phycodnaviridae; Chlorovirus; Virus PBCV-1; Virus NY-2A; Virus MT325; Promoters INTRODUCTION Chlorella viruses (Family Phycodnaviridae, genus

Graves, Michael V.

475

E-Print Network 3.0 - amv cucumber mosaic Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Summary: including alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV) and Potato virus Y (PVY). At this time... fields of pumpkin, melon, or cucumber consider using...

476

Purification and characterization of ORF69 of Autographa californica nuclear polyhedrosis virus  

E-Print Network [OSTI]

provided a powerful model system for genetic analysis of cap synthesis and the role of the cap in cellular metabolism (Shibagaki et al. , 1992; Mao et al. , 1995; Tsukamoto et al. , 1997). The yeast cap MTase ABDI gene was identified on the basis of its...

Wu, Xiaofeng

2002-01-01T23:59:59.000Z

477

Virus-enabled synthesis and 2D/3D assembly of nanowire networks  

E-Print Network [OSTI]

Dense networks of high aspect ratio nanowires can provide important functionality to electronic devices through a unique combination of electronic and structural properties including high conductivity, high surface area, ...

Liau, Forrest (Forrest W.)

2013-01-01T23:59:59.000Z

478

E-Print Network 3.0 - atenuada del virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Very Large Telescope (VLT) y el Gran ... Source: Canarias, Instituto de Astrofisica de (IAC) Collection: Physics 4 Ciclo de vida del VIH Enlace y fusin: El VIH empieza su ciclo de...

479

adeno-associated virus-mediated delivery: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

for Different Contexts Tayeb Lemlouma1 and Nabil Layada2 OPERA Project present a framework which allows adapted content delivery for different target contexts. This...

480

Induction of mutations resistant to maize dwarf mosaic virus in Sorghum bicolor using gamma radiation  

E-Print Network [OSTI]

in a greenhouse which was kept at approximately 22 C. Water and nutrients were supplied, as needed. Fourteen days (three-leaf stage) after planting, the seedlings were hand-inoculated with MDMV-A. 1he inoculum was made in the manner previously... in NM-31. Two independent mutations which confer a high degree of MDNV resistance were identified and confirmed by progeny tests. Electron microscopic sections of the two con- firmed resistant mutants (M-380-13 and N-369-2) taken 336 hours after...

Pye, Quentin Niel

1977-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "outsmarting flu viruses" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

E-Print Network 3.0 - af louping ill-virus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

issue: www.prcboston.orgnewslettersloupeLoupev2n1.pdf ScheduleOctober 2011-June 2012 Source: Finzi, Adrien - Department of Biology, Boston University Collection:...

482

[23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression  

E-Print Network [OSTI]

RNA). In cultured mammalian cells and in whole animals, infection with these vectors was shown to result in specific vehicles. Introduction: Exploiting RNA Interference (RNAi)--Promises and Problems Over the past 3 years that mammalian cells are capable of eliciting the sequence- specific posttranscriptional gene silencing pathway

Ford, James

483

Biopolymers Under Large External Forces and Mean-field RNA Virus Evolutionary Dynamics  

E-Print Network [OSTI]

H. Yin, R. Landick, J. Gelles, and S. M. Block, Force andH. Yin, R. Landick, J. Gelles, and S. M. Block. StretchingH. Yin, R. Landick, J. Gelles, and S. M. Block. Stretching

Ahsan, Syed Amir

2013-01-01T23:59:59.000Z

484

E-Print Network 3.0 - attenuated bluetongue virus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

sensitive to environmental and climatic factors.24 Bluetongue ... Source: Singer, Randall - College of Veterinary Medicine, University of Minnesota Collection: Biology and...

485

Infected Lives: A Heideggerian Phenomenological Study of Young African American Human Immunodeficiency Virus-Positive Women  

E-Print Network [OSTI]

and discriminated against in their interpersonal and social systems. They often faced myriad, unresolved conflicts. They were coping with unrelenting depression and other physical symptoms. Implications for education, practice, policy and research are discussed....

Peltzer, Jill Nicole

2012-05-31T23:59:59.000Z

486

The effects of maize dwarf mosaic virus strain B on sorghum  

E-Print Network [OSTI]

. Transmission of MDMV-8 by aphids has been demonstrated (23, 34), and there is evidence to support the theory that aphid flights can carry and transmit MDMV-B to hosts hundreds of kilometers from an inoculum source (2). Seed transmission has also been...

Alexander, John David

2012-06-07T23:59:59.000Z

487

Influence of Surface Protein V6 Region of Equine Infectious Anemia Virus on Cytokine Gene Expression  

E-Print Network [OSTI]

............................................................................................................. 11 1.3.1.3 Env ........................................................................................................... 12 1.3.1.4. Regulatory Proteins ................................................................................. 13 1...}. The EIAV surface projections are composed of two env- 9 encoded glycoproteins, a heavily glycosylated protein of 90kDa (gp90) and a membrane spanning 45kDa glycoprotein (gp45) {Montelaro et al., 1993}. EIAV gp90 is now commonly referred...

Lamon, Tennille Krystal

2014-08-14T23:59:59.000Z

488

Development of VIRUS Alignment and Assembly Fixtures Amanda D. Collins1  

E-Print Network [OSTI]

Energy Experiment (HETDEX). We plan to use a production line assembly process to construct the large Villanueva, Jr.1 1 Department of Physics and Astronomy, Texas A&M University, 4242 TAMU, College Station, TX, USA 77843-4242 2 McDonald Observatory, University of Texas at Austin, 1 University Station, C1402

489

Quantitatively Depositing Viruses onto TEM Grids Jennifer Brum, Tucson Marine Phage Lab  

E-Print Network [OSTI]

-sided tape with a razor blade b) stick the strips to the top, interior, distal surface of the rotor chambers deposited onto the grids 5) Add 100 µl of your sample to the rotor chambers a) the rotor MUST be balanced) - if >100 µl is required, do multiple Airfuge runs on the same grid, replacing the sample in the rotor

Sullivan, Matthew B.

490

A study of the growth cycle and antigenic structure of bluetongue virus in tissue culture systems  

E-Print Network [OSTI]

A STUJI OF T'4 '9'. . i C Cl ' A:8 AUTlC ' iIC S 'IUC i. ". '. ' OF U. U ";O'GJU VZ IJS I, TI&2&~~ C UnYU ~ ' i~S I ~ ) CIA:~LuS C ~ LIVI JG810U& Ji' ~ Sxb. dtted to the Greducte School of the ACric&turcl cnd . '. schcniccl Collepe oi' Tcx;. e... to susceptible a:dzxals by contact with infocted motorial or b. ; drenching with vix-. Q. ont bloc@ I {19'35) in South Afl'ica Made ti18 first detailed st'2+ of bluet c ~~i . !o ao tonstrat. d that it;au:ux lx&ocul bio discase, t!xe causative agent o vi -xc...

Livingston, Charles Wesley, Jr

1961-01-01T23:59:59.000Z

491

Multiresolution Approaches to Representation and Visualization of Large Influenza Virus Sequence Datasets  

E-Print Network [OSTI]

Datasets Leonid Zaslavsky National Center for Biotechnology Information National Library of Medicine dataset is characterized by low estimated values of the Kolmogorov (box) dimension. Multi- scale methodologies allow interactive visual representation of the dataset and accelerate computations by importance

Levin, Judith G.

492

The Potential for Activated Biochar to Remove Waterborne Viruses from Environmental Waters  

E-Print Network [OSTI]

of biofuels. These interests have led to not only the examination of current method limitations, but also to the investigation of new conversion methods. One promising method for bioenergy production is pyrolysis of lignocellulosic feedstocks. Through...

Florey, James

2012-07-16T23:59:59.000Z

493

Identification and characterization of a virus isolated from bobwhite quail with a respiratory disease  

E-Print Network [OSTI]

$ Desori t 0 of the Diss ~ The outbreak of quail bronchitis reported by Olson ooeurre4 21 in 1949 at the Best Virgixd. a State Game Farm. Coughing and unseeing was observed in some of the adult bobshite quail, In young bixds spnptons first eccurred... at $ weeks of age. Foe4 con suaptien droype4 and coughing, encasing an4 tales were noto4. Basal disoharge was not seen. Bending cf the neck between tho wings or legs occurred in 2 to ) yoreent of the birds. The course of the dieoase was 1 to $ weeks...

DuBose, Robert Trafton

1958-01-01T23:59:59.000Z

494

ThisprototypeT-cellvaccinecontainscom-ponents from multiple genes of the virus --the  

E-Print Network [OSTI]

the world. It will be tested in partnership with the Division of AIDS in NIAID and three clinical trial networks in dif- ferent regions of the world. Phase II studies have begun at most of these sites and that would greatly accelerate futurehumanclinicaltrials. Outlook What does the future hold for HIV vaccine

Cai, Long

495

Using remote sensing to map the risk of human monkeypox virus in the Congo basin  

E-Print Network [OSTI]

We conducted a geospatial analysis using existing data toHere we conduct a geospatial analysis of surveillance data

2011-01-01T23:59:59.000Z

496

Isolation methods and electron microscopy of the Internal Cork Virus of sweet potatoes  

E-Print Network [OSTI]

and then diluting to 100 milli- liters with distilled water to make the buffered solution that was used during grinding of the lesions. After grinding was completed the slurry was squeezed through cheese cloth to remove the gross material. The slurry passing... in each of' the three tubes was resuspended in one milliliter of solution containing 0. 5 grams of sodium bromide per milliliter (14). Each of the three, one milliliter suspensions was transferred to a centrifuge tube for the SW39 rotor designed f...

Pickens, Edgar Eugene

1967-01-01T23:59:59.000Z

497

Development of Novel Diagnostic Tools and beyond for Targets of Human Significance: Metabolites, Toxins & Viruses  

E-Print Network [OSTI]

0.1 mM MnCl2 and 0.5 mM CaCl2 prepared in 10 mM phosphate0.1 mM MnCl2 and 0.5 mM CaCl2 prepared in 10 mM phosphatesupplemented with 0.5 mM CaCl2 and 0.1 mM MnCl2 (PB) for 2 h

Cella, Lakshminarasamamba

2012-01-01T23:59:59.000Z

498

E-Print Network 3.0 - anemia virus infection Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of - Department of Electrical and Computer Engineering, Performability Engineering Research Group Collection: Computer Technologies and Information Sciences 42 Recent...

499

Natural and experimental host range of the Maize Dwarf Mosaic Virus in Texas  

E-Print Network [OSTI]

Panicum antidotale Reta (blue panicum)(W) Seghal (30) P~L L. ( (l hb . ) (M) Bancroft et al. (3) Ford (14) e. , S +, S Pas alum notatum Flugge var. notatum (Bahiagraas) (W) Bancroft et al. (3) -, NS Ph laris steno tera Hack (Harding grass...

Leisy, Ralph Herbert

1968-01-01T23:59:59.000Z

500

VIROLOGY 163, 319-329 (1988) Genetic identification of a Portion of the Herpes Simplex Virus  

E-Print Network [OSTI]

., 1982b). The binding of ICP8 to DNA is cooperative under cer- tain conditions in vido (Ruyechan, 1983

Knipe, David M.