National Library of Energy BETA

Sample records for organisms synthetic biology

  1. Repurposing the translation apparatus for synthetic biology ...

    Office of Scientific and Technical Information (OSTI)

    biology Citation Details In-Document Search This content will become publicly available on July 14, 2017 Title: Repurposing the translation apparatus for synthetic biology ...

  2. Copy of Synthetic Biology of Novel Thermophilic Bacteria for...

    Office of Scientific and Technical Information (OSTI)

    Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ... Title: Copy of Synthetic Biology of Novel Thermophilic Bacteria for Enhanced Production of ...

  3. Synthetic Biology for Advanced Fuels (Opening Keynote Address...

    Office of Scientific and Technical Information (OSTI)

    Synthetic Biology for Advanced Fuels (Opening Keynote Address - 2010 JGI User Meeting) Citation Details In-Document Search Title: Synthetic Biology for Advanced Fuels (Opening ...

  4. Micro/nanofabricated environments for synthetic biology

    SciTech Connect (OSTI)

    Collier, Pat [ORNL; Simpson, Michael L [ORNL

    2011-01-01

    A better understanding of how confinement, crowding and reduced dimensionality modulate reactivity and reaction dynamics will aid in the rational and systematic discovery of functionality in complex biological systems. Artificial micro- and nanofabricated structures have helped elucidate the effects of nanoscale spatial confinement and segregation on biological behavior, particularly when integrated with microfluidics, through precise control in both space and time of diffusible signals and binding interactions. Examples of nanostructured interfaces for synthetic biology include the development of cell-like compartments for encapsulating biochemical reactions, nanostructured environments for fundamental studies of diffusion, molecular transport and biochemical reaction kinetics, and regulation of biomolecular interactions as functions of micro- and nanofabricated topological constraints.

  5. Synthetic biology and crop engineering | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic biology and crop engineering Synthetic biology and crop engineering Breakout Session 2: Frontiers and Horizons Session 2-A: Synthetic Biology and the Promise of Biofuels Jonathan Burbaum, Program Director, Department of Energy, Office of Science, ARPA-E b13_burbaum_2-a.pdf (1.63 MB) More Documents & Publications EIS-0481: Final Programmatic Environmental Impact Statement EIS-0481: Draft Programmatic Environmental Impact Statement EIS-0481: Notice of Intent to Prepare a Programmatic

  6. Synthetic biology for microbial production of lipid-based biofuels...

    Office of Scientific and Technical Information (OSTI)

    Synthetic biology for microbial production of lipid-based biofuels Citation Details In-Document Search This content will become publicly available on October 22, 2017 Title: ...

  7. Renewable Energy from Synthetic Biology (LBNL Science at the Theater)

    ScienceCinema (OSTI)

    Keasling, Jay

    2011-04-28

    Jay Keasling, co-leader of Berkeley Lab's Helios Project, is a groundbreaking researcher in the new scientific field of synthetic biology. In Helios, he directs the biology program, incorporating a range of approaches to increasing the efficacy and economy of plants and cellulose-degrading microbes to make solar-based fuels. He is a UC Berkeley professor of Chemical and Bioengineering, and founder of Amyris Biotechnologies, a company that was honored as a Technology Pioneer for 2006 by the World Economic Forum. Keasling has succeeded in using synthetic biology to develop a yeast-based production scheme for precursors of the antimalarial drug artemisinin in work funded by the Bill & Melinda Gates Foundation.

  8. Synthetic Biology: Engineering, Evolution and Design (SEED) Conference 2014

    SciTech Connect (OSTI)

    Voigt, Christopher

    2014-07-01

    SEED2014 focused on advances in the science and technology emerging from the field of synthetic biology. We broadly define this as technologies that accelerate the process of genetic engineering. It highlighted new tool development, as well as the application of these tools to diverse problems in biotechnology, including therapeutics, industrial chemicals and fuels, natural products, and agriculture. Systems spanned from in vitro experiments and viruses, through diverse bacteria, to eukaryotes (yeast, mammalian cells, plants).

  9. Synthetic biological membrane with self organizing properties

    DOE Patents [OSTI]

    Firestone, Millicent A.; Tiede, David M.

    2003-01-01

    A mixture is provided which manifests a gel phase at a temperature higher than that in which the mixture manifests a liquid phase. The mixture is a combination of a lipid, a polymer-grafted phospholipid and a surfactant. It is biomimetic in nature and changes phases when subjected to one or a plurality of environmental stimuli.

  10. Synthetic Biology and the U.S. Biotechnology Regulatory System: Challenges and Options

    SciTech Connect (OSTI)

    Carter, Sarah R.; Rodemeyer, Michael; Garfinkel, Michele S.; Friedman, Robert M

    2014-05-01

    Synthetic Biology and the U.S. Biotechnology Regulatory System: Challenges and Options Sarah R. Carter, Ph.D., J. Craig Venter Institute; Michael Rodemeyer, J.D., University of Virginia; Michele S. Garfinkel, Ph.D., EMBO; Robert M. Friedman, Ph.D., J. Craig Venter Institute In recent years, a range of genetic engineering techniques referred to as “synthetic biology” has significantly expanded the tool kit available to scientists and engineers, providing them with far greater capabilities to engineer organisms than previous techniques allowed. The field of synthetic biology includes the relatively new ability to synthesize long pieces of DNA from chemicals, as well as improved methods for genetic manipulation and design of genetic pathways to achieve more precise control of biological systems. These advances will help usher in a new generation of genetically engineered microbes, plants, and animals. The JCVI Policy Center team, along with researchers at the University of Virginia and EMBO, examined how well the current U.S. regulatory system for genetically engineered products will handle the near-term introduction of organisms engineered using synthetic biology. In particular, the focus was on those organisms intended to be used or grown directly in the environment, outside of a contained facility. The study concludes that the U.S. regulatory agencies have adequate legal authority to address most, but not all, potential environmental, health and safety concerns posed by these organisms. Such near-term products are likely to represent incremental changes rather than a marked departure from previous genetically engineered organisms. However, the study also identified two key challenges for the regulatory system, which are detailed in the report. First, USDA’s authority over genetically engineered plants depends on the use of an older engineering technique that is no longer necessary for many applications. The shift to synthetic biology and other newer genetic

  11. Micro/nanofabricated environments for synthetic biology (Journal...

    Office of Scientific and Technical Information (OSTI)

    Artificial micro- and nanofabricated structures have helped elucidate the effects of nanoscale spatial confinement and segregation on biological behavior, particularly when ...

  12. End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Linshiz, Gregory; Jensen, Erik; Stawski, Nina; Bi, Changhao; Elsbree, Nick; Jiao, Hong; Kim, Jungkyu; Mathies, Richard; Keasling, Jay D.; Hillson, Nathan J.

    2016-02-02

    Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. Here, we present a programmable, multipurpose microfluidic platform and associated software and apply the platform to major steps of the synthetic biology research cycle: design, construction, testing, and analysis. We show the platform’s capabilities for multiple automated DNA assembly methods, including a new method for Isothermal Hierarchical DNA Construction, and for Escherichia coli and Saccharomyces cerevisiae transformation. The platform enables the automated control of cellular growth, gene expression induction, andmore » proteogenic and metabolic output analysis. Finally, taken together, we demonstrate the microfluidic platform’s potential to provide end-to-end solutions for synthetic biology research, from design to functional analysis.« less

  13. SB6.0: The 6th International meeting on Synthetic Biology, July 9-11, 2013

    SciTech Connect (OSTI)

    Kahl, Linda J.

    2015-04-23

    The Synthetic Biology conference series (SBx.0) is the preeminent academic meeting in synthetic biology. Organized by the BioBricks Foundation, the SBx.0 conference series brings together leading researchers, students, industry executives, and policy makers from around the world to share, consider, debate, and plan efforts to make biology easier to engineer. Historically held every two years, the SBx.0 conferences are held in alternating locations in the United States, Europe, and Asia to encourage global participation and collaboration so that the ramifications of synthetic biology research and development are most likely to be safe ethical, and beneficial. On 9-11 July 2013, the 6th installment of the synthetic biology conference series (SB6.0) was held on the campus of Imperial College London (http://sb6.biobricks.org). The SB6.0 conference was attended by over 700 people, and many more were able to participate via video digital conference (http://sb6.biobricks.org/digital-conference/). Over the course of three days, the SB6.0 conference agenda included plenary sessions, workshops, and poster presentations covering topics ranging from the infrastructure needs arising when “Systematic Engineering Meets Biological Complexity” and design-led considerations for “Connecting People and Technologies” to discussions on “Engineering Biology for New Materials,” “Assessing Risk and Managing Biocontainment,” and “New Directions for Energy and Sustainability.” The $10,150 grant awarded by the U.S. Department of Energy (DE-SC0010233) to the BioBricks Foundation was used to provide partial reimbursement for the travel expenses of leading researchers from the United States to speak at the SB6.0 conference. A total of $9,450 was used to reimburse U.S. speakers for actual expenses related to the SB6.0 conference, including airfare (economy or coach only), ground transportation, hotel, and registration fees. In addition, $700 of the grant was used to offset

  14. Synthetic biology R&D risks: Social-institutional contexts matter!

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Wolfe, Amy K.; Bjornstad, David J.; Shumpert, Barry L.; Campa, Maria Fernanda; Bergmann, Rachael A.; Stelling, Savannah C.

    2016-02-15

    Social and institutional analyses currently are missing from considerations of synthetic biology R&D-related biosafety, which instead have bioethics, governance, or technical orientations. Social and institutional context shapes standard practice. Here, analyzing context helps identify circumstances that create, amplify, or diminish risk, thereby revealing new opportunities for avoiding or managing those risks.

  15. Synthetic Biology for Tailored Enzyme Cocktails Presentation for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    March 2015 SynTec Synthetic Biology for Tailored Enzyme Cocktails DOE Bioenergy Technologies Office (BETO) 2015 Project Peer Review Biochemical Conversion Sarah Teter Novozymes, Inc This presentation does not contain any proprietary, confidential, or otherwise restricted information NOVOZYMES PRESENTATION 26 Mar 2015 2 ACCELERATE INNOVATION IN ENZYME DISCOVERY REDUCE COSTS FOR PRODUCING CELLULOSIC SUGARS  Deliver a screening platform which can be used to reduce discovery time required for

  16. Part Mining for Synthetic Biology (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Voigt, Chris [MIT

    2013-03-01

    Chris Voigt from MIT delivers the opening keynote on "Part Mining for Synthetic Biology" at the 8th Annual Genomics of Energy & Environment Meeting on March 26, 2013 in Walnut Creek, Calif.

  17. Methods for isolation and viability assessment of biological organisms

    DOE Patents [OSTI]

    Letant, Sonia Edith; Baker, Sarah Elyse; Bond, Tiziana; Chang, Allan Shih-Ping

    2015-02-03

    Isolation of biological or chemical organisms can be accomplished using a surface enhanced Raman scattering (SERS) system. The SERS system can be a single or a stacked plurality of photonic crystal membranes with noble-metal lined through pores for flowing analyte potentially containing the biological or chemical organisms. The through pores can be adapted to trap individual biological or chemical organisms and emit SERS spectra, which can then be detected by a detector and further analyzed for viability of the biological or chemical organism.

  18. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  19. Synthetic Metagenomics: Converting digital information back to Biology (2013 DOE JGI Genomics of Energy and Environment 8th Annual User Meeting)

    SciTech Connect (OSTI)

    Deutsch, Sam [DOE Joint Genome Institute

    2013-03-01

    Sam Deutsch of the DOE JGI on "Synthetic Metagenomics: Converting digital information back to Biology" at the 8th Annual Genomics of Energy & Environment Meeting in Walnut Creek, Calif.

  20. Synthetic fossilization of soft biological tissues and their shape-preserving transformation into silica or electron-conductive replicas

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Townson, Jason L.; Lin, Yu-Shen; Chou, Stanley S.; Awad, Yasmine H.; Coker, Eric N.; Brinker, C. Jeffrey; Kaehr, Bryan

    2014-12-08

    Structural preservation of complex biological systems from the subcellular to whole organism level in robust forms, enabling dissection and imaging while preserving 3D context, represents an enduring grand challenge in biology. Here we report a simple immersion method for structurally preserving intact organisms via conformal stabilization within silica. This self-limiting process, which we refer to as silica bioreplication, occurs by condensation of water-soluble silicic acid proximally to biomolecular interfaces throughout the organism. Conformal nanoscopic silicification of all biomolecular features imparts structural rigidity enabling the preservation of shape and nano-to-macroscale dimensional features upon drying to form a biocomposite and further highmore » temperature oxidative calcination to form silica replicas or reductive pyrolysis to form electrically conductive carbon replicas of complete organisms. Ultimately, the simplicity and generalizability of this approach should facilitate efforts in biological preservation and analysis and could enable the development of new classes of biomimetic composite materials.« less

  1. Synthetic fossilization of soft biological tissues and their shape-preserving transformation into silica or electron-conductive replicas

    SciTech Connect (OSTI)

    Townson, Jason L.; Lin, Yu-Shen; Chou, Stanley S.; Awad, Yasmine H.; Coker, Eric N.; Brinker, C. Jeffrey; Kaehr, Bryan

    2014-12-08

    Structural preservation of complex biological systems from the subcellular to whole organism level in robust forms, enabling dissection and imaging while preserving 3D context, represents an enduring grand challenge in biology. Here we report a simple immersion method for structurally preserving intact organisms via conformal stabilization within silica. This self-limiting process, which we refer to as silica bioreplication, occurs by condensation of water-soluble silicic acid proximally to biomolecular interfaces throughout the organism. Conformal nanoscopic silicification of all biomolecular features imparts structural rigidity enabling the preservation of shape and nano-to-macroscale dimensional features upon drying to form a biocomposite and further high temperature oxidative calcination to form silica replicas or reductive pyrolysis to form electrically conductive carbon replicas of complete organisms. Ultimately, the simplicity and generalizability of this approach should facilitate efforts in biological preservation and analysis and could enable the development of new classes of biomimetic composite materials.

  2. Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    King, Travis L.; Gatimu, Enid N.; Bohn, Paul W.

    2009-01-02

    This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e.,DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanoporesmore » using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d > 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.« less

  3. New Synthetic Methods for Hypericum Natural Products

    SciTech Connect (OSTI)

    Insik Jeon

    2006-12-12

    Organic chemistry has served as a solid foundation for interdisciplinary research areas, such as molecular biology and medicinal chemistry. An understanding of the biological activities and structural elucidations of natural products can lead to the development of clinically valuable therapeutic options. The advancements of modern synthetic methodologies allow for more elaborate and concise natural product syntheses. The theme of this study centers on the synthesis of natural products with particularly challenging structures and interesting biological activities. The synthetic expertise developed here will be applicable to analog syntheses and to other research problems.

  4. A synthetic ecology perspective: How well does behavior of model organisms in the laboratory predict microbial activities in natural habitats?

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yu, Zheng; Krause, Sascha M. B.; Beck, David A. C.; Chistoserdova, Ludmila

    2016-06-15

    In this perspective article, we question how well model organisms, the ones that are easy to cultivate in the laboratory and that show robust growth and biomass accumulation, reflect the dynamics and interactions of microbial communities observed in nature. Today's -omics toolbox allows assessing the genomic potential of microbes in natural environments in a high-throughput fashion and at a strain-level resolution. However, understanding of the details of microbial activities and of the mechanistic bases of community function still requires experimental validation in simplified and fully controlled systems such as synthetic communities. We have studied methane utilization in Lake Washington sedimentmore » for a few decades and have identified a number of species genetically equipped for this activity. We have also identified cooccurring satellite species that appear to form functional communities together with the methanotrophs. Here, we compare experimental findings from manipulation of natural communities involved in metabolism of methane in this niche with findings from manipulation of synthetic communities assembled in the laboratory of species originating from the same study site, from very simple (two-species) to rather complex (50-species) synthetic communities. We observe some common trends in community dynamics between the two types of communities, toward representation of specific functional guilds. However, we also identify strong discrepancies between the dominant methane oxidizers in synthetic communities compared to natural communities, under similar incubation conditions. Furthermore, these findings highlight the challenges that exist in using the synthetic community approach to modeling dynamics and species interactions in natural communities.« less

  5. Photoconversion of gasified organic materials into biologically-degradable plastics

    DOE Patents [OSTI]

    Weaver, P.F.; Pinching Maness.

    1993-10-05

    A process is described for converting organic materials (such as biomass wastes) into a bioplastic suitable for use as a biodegradable plastic. In a preferred embodiment the process involves thermally gasifying the organic material into primarily carbon monoxide and hydrogen, followed by photosynthetic bacterial assimilation of the gases into cell material. The process is ideally suited for waste recycling and for production of useful biodegradable plastic polymer. 3 figures.

  6. Photoconversion of gasified organic materials into biologically-degradable plastics

    DOE Patents [OSTI]

    Weaver, Paul F.; Maness, Pin-Ching

    1993-01-01

    A process is described for converting organic materials (such as biomass wastes) into a bioplastic suitable for use as a biodegradable plastic. In a preferred embodiment the process involves thermally gasifying the organic material into primarily carbon monoxide and hydrogen, followed by photosynthetic bacterial assimilation of the gases into cell material. The process is ideally suited for waste recycling and for production of useful biodegradable plastic polymer.

  7. LANL organic analysis detection capabilities for chemical and biological warfare agents

    SciTech Connect (OSTI)

    Ansell, G.B.; Cournoyer, M.E.; Hollis, K.W.; Monagle, M.

    1996-12-31

    Organic analysis is the analytical arm for several Los Alamos National Laboratory (LANL) research programs and nuclear materials processes, including characterization and certification of nuclear and nonnuclear materials used in weapons, radioactive waste treatment and waste certification programs. Organic Analysis has an extensive repertoire of analytical technique within the group including headspace gas, PCBs/pesticides, volatile organics and semivolatile organic analysis. In addition organic analysis has mobile labs with analytic capabilities that include volatile organics, total petroleum hydrocarbon, PCBs, pesticides, polyaromatic hydrocarbons and high explosive screening. A natural extension of these capabilities can be applied to the detection of chemical and biological agents,

  8. Fibrillar Organic Phases And Their Roles In Rigid Biological Composites

    SciTech Connect (OSTI)

    Arey, Bruce W.; Park, John J.; Mayer, George

    2015-06-01

    This study focused on determining the presence of organic phases in the siliceous components of rigid marine composites ("glass" sponge spicules), and thereby to clarify how those composites dissipate significant mechanical energy. Through the use of imaging by helium ion microscopy in the examination of the spicules, the organic phase that is present between the layers of hydrated silica was also detected within the silica cylinders of the composite, indicating the existence therein of a network, scaffolding, or other pattern that has not yet been determined. It was concluded that the presence of an interpenetrating network of some kind, and tenacious fibrillar interfaces are responsible for the large energy dissipation in these siliceous composites by viscoelastic processes. This discovery means that future mechanics analyses of such composites, extending to large deformations must consider such interpenetrating phases.

  9. Lateral organization, bilayer asymmetry, and inter-leaflet coupling of biological membranes

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Smith, Jeremy C.; Cheng, Xiaolin; Nickels, Jonathan D.

    2015-07-29

    Understanding of cell membrane organization has evolved significantly from the classic fluid mosaic model. It is now recognized that biological membranes are highly organized structures, with differences in lipid compositions between inner and outer leaflets and in lateral structures within the bilayer plane, known as lipid rafts. These organizing principles are important for protein localization and function as well as cellular signaling. However, the mechanisms and biophysical basis of lipid raft formation, structure, dynamics and function are not clearly understood. One key question, which we focus on in this review, is how lateral organization and leaflet compositional asymmetry are coupled.more » Detailed information elucidating this question has been sparse because of the small size and transient nature of rafts and the experimental challenges in constructing asymmetric bilayers. Resolving this mystery will require advances in both experimentation and modeling. We discuss here the preparation of model systems along with experimental and computational approaches that have been applied in efforts to address this key question in membrane biology. Furthermore, we seek to place recent and future advances in experimental and computational techniques in context, providing insight into in-plane and transverse organization of biological membranes.« less

  10. Lateral organization, bilayer asymmetry, and inter-leaflet coupling of biological membranes

    SciTech Connect (OSTI)

    Smith, Jeremy C.; Cheng, Xiaolin; Nickels, Jonathan D.

    2015-07-29

    Understanding of cell membrane organization has evolved significantly from the classic fluid mosaic model. It is now recognized that biological membranes are highly organized structures, with differences in lipid compositions between inner and outer leaflets and in lateral structures within the bilayer plane, known as lipid rafts. These organizing principles are important for protein localization and function as well as cellular signaling. However, the mechanisms and biophysical basis of lipid raft formation, structure, dynamics and function are not clearly understood. One key question, which we focus on in this review, is how lateral organization and leaflet compositional asymmetry are coupled. Detailed information elucidating this question has been sparse because of the small size and transient nature of rafts and the experimental challenges in constructing asymmetric bilayers. Resolving this mystery will require advances in both experimentation and modeling. We discuss here the preparation of model systems along with experimental and computational approaches that have been applied in efforts to address this key question in membrane biology. Furthermore, we seek to place recent and future advances in experimental and computational techniques in context, providing insight into in-plane and transverse organization of biological membranes.

  11. A Versatile Synthetic Route for the Preparation of Titanium Metal...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Versatile Synthetic Route for the Preparation of Titanium Metal-Organic Frameworks ... A Versatile Synthetic Route for the Preparation of Titanium Metal-Organic Frameworks. ...

  12. INFLUENCE OF NATURAL AND SYNTHETIC ORGANIC LIGANDS ON THE STABILITY AND MOBILITY OF REDUCED TC(IV)

    SciTech Connect (OSTI)

    Nathalie A. Wall; Baohua Gu

    2012-12-20

    The primary objectives were (1) to quantify the interactions of organic ligands with Tc(IV) through the generation of thermodynamic (complexation) and kinetic parameters needed to assess and predict the mobility of reduced Tc(IV) at DOE contaminated sites; and (2) to determine the impact of organic ligands on the mobility and fate of reduced Tc(IV) under field geochemical conditions.

  13. Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biology @WIPP Life Begins at 250,000,000 Years WIPP's underground isn't just suited for physics experiments aiming to unlock the mysteries of the Universe, it is also a perfect "dig site" for biologists wanting to chronicle the history of life. 250 million years ago, the area around WIPP was all part of the Permian Sea. Today, the salt beds that make up the WIPP underground provide a time capsule, of sorts, to this ancient era. Researchers have uncovered ancient bacteria, cellulose and

  14. Synthetic fuels

    SciTech Connect (OSTI)

    Not Available

    1989-01-01

    In January 1982, the Department of Energy guaranteed a loan for the construction and startup of the Great Plains project. On August 1, 1985, the partnership defaulted on the $1.54 billion loan, and DOE acquired control of, and then title to, the project. DOE continued to operate the plant, through the ANG Coal Gasification Company, and sell synthetic fuel. The DOE's ownership and divestiture of the plant is discussed.

  15. A model for improving microbial biofuel production using a synthetic...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Synthetic and Systems Biology; Journal Volume: 4; Related Information: Journal Publication Date: 25 FEB 2010 ...

  16. Synthetic analogs of bacterial quorum sensors

    DOE Patents [OSTI]

    Iyer, Rashi S.; Ganguly, Kumkum; Silks, Louis A.

    2013-01-08

    Bacterial quorum-sensing molecule analogs having the following structures: ##STR00001## and methods of reducing bacterial pathogenicity, comprising providing a biological system comprising pathogenic bacteria which produce natural quorum-sensing molecule; providing a synthetic bacterial quorum-sensing molecule having the above structures and introducing the synthetic quorum-sensing molecule into the biological system comprising pathogenic bacteria. Further is provided a method of targeted delivery of an antibiotic, comprising providing a synthetic quorum-sensing molecule; chemically linking the synthetic quorum-sensing molecule to an antibiotic to produce a quorum-sensing molecule-antibiotic conjugate; and introducing the conjugate into a biological system comprising pathogenic bacteria susceptible to the antibiotic.

  17. Synthetic analogs of bacterial quorum sensors

    DOE Patents [OSTI]

    Iyer, Rashi; Ganguly, Kumkum; Silks, Louis A.

    2011-12-06

    Bacterial quorum-sensing molecule analogs having the following structures: ##STR00001## and methods of reducing bacterial pathogenicity, comprising providing a biological system comprising pathogenic bacteria which produce natural quorum-sensing molecule; providing a synthetic bacterial quorum-sensing molecule having the above structures and introducing the synthetic quorum-sensing molecule into the biological system comprising pathogenic bacteria. Further is provided a method of targeted delivery of an antibiotic, comprising providing a synthetic quorum-sensing molecule; chemically linking the synthetic quorum-sensing molecule to an antibiotic to produce a quorum-sensing molecule-antibiotic conjugate; and introducing the conjugate into a biological system comprising pathogenic bacteria susceptible to the antibiotic.

  18. Development and Applications Of Photosensitive Device Systems To Studies Of Biological And Organic Materials

    SciTech Connect (OSTI)

    Gruner, Sol

    2012-01-20

    The primary focus of the grant is the development of new x-ray detectors for biological and materials work at synchrotron sources, especially Pixel Array Detectors (PADs), and the training of students via research applications to problems in biophysics and materials science using novel x-ray methods. This Final Progress Report provides a high-level overview of the most important accomplishments. These major areas of accomplishment include: (1) Development and application of x-ray Pixel Array Detectors; (2) Development and application of methods of high pressure x-ray crystallography as applied to proteins; (3) Studies on the synthesis and structure of novel mesophase materials derived from block co-polymers.

  19. 3D Reconstruction of Biological Organization and Mineralization in Sediment Attached Biofilms During Uranium Bioremediation

    SciTech Connect (OSTI)

    Banfield, Jillian; Comolli, Luis R.; Singer, Steve

    2014-11-17

    Of central interest in this study were microbial communities attached to sediment particles and in associated groundwater. Are the communities similar? What do the organisms look like and how do they associated with each other? Research was conducted on samples collected from the Department of Energy's (DOE) Rifle Integrated Field Research Challenge (IFRC) site in Rifle, Colorado, USA. This site served first as a test case for in situ bioremediation via biostimulation, and more recently as a location for studying the role of microbial communities in the carbon and other linked biogeochemical cycles. We addressed the question of the nature of planktonic to sediment-attached microbial communities using field and laboratory experimental studies with a range of methods that provide 2- and 3-dimensional topological information coupled to information about chemical speciation, organism type, and activity levels. The research leveraged data from metagenomics and proteomics analyses that obtained through parallel work at the Rifle site in the context of the IFRC. In this project we integrated characterization methods with extensive genomic sequence information and associated environmental data to better understand the processes that occur within groundwater and sediment-attached communities. Notable is the range of characterization methods, including scanning transmission x-ray microscopy (STXM), micro-EXAFS/XANES and microdiffraction and 2D and 3D cryo-electron tomographic analysis, and high-resolutino transmission electron microscope (HRTEM) analysis to characterize these natural microbial communities. A cryo-TEM work was unique because samples for electron microscopic characterization were cryo-plunged directly on site immediately after sampling. This step minimizes post-collection alterations, including cell damages and change of redox state. Among many achievements documented in publications listed at the end of this report, we highlight the following: 1) The

  20. Biochemical Process Improvements & Synthetic Biology Validation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Project m anagement o ccurs within the context of NREL's Biomass Program AOP, with ... reasonableness of techno---economic modeling approach and establish benchmark cost. ...

  1. Synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Pena, Louis A.; Zamora, Paul; Lin, Xinhua; Glass, John D.

    2007-01-23

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain that binds a heparin-binding growth factor receptor, covalently bound to a hydrophobic linker, which is in turn covalently bound to a non-signaling peptide that includes a heparin-binding domain. The synthetic heparin-binding growth factor analogs are useful as soluble biologics or as surface coatings for medical devices.

  2. Synthetic and Mechanistic Chemistry publications

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Synthetic and Mechanistic Chemistry Synthetic and Mechanistic Synthetic and Mechanistic ... Get Expertise Dave Thorn Chemistry Program Manager Email Josh Smith Chemistry ...

  3. Synthetic and Mechanistic Chemistry

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Synthetic and Mechanistic Chemistry Synthetic and Mechanistic Chemistry Synthetic and mechanistic chemical sciences play an important role in Lab missions dedicated to energy security, emerging challenges, global security, and core weapons missions. Get Expertise Dave Thorn Chemistry Program Manager Email Josh Smith Chemistry Communications Email "Research into alternative forms of energy, of which biofuels is a key component, is one of the major national security imperatives of this

  4. Biological conversion system

    DOE Patents [OSTI]

    Scott, C.D.

    A system for bioconversion of organic material comprises a primary bioreactor column wherein a biological active agent (zymomonas mobilis) converts the organic material (sugar) to a product (alcohol), a rejuvenator column wherein the biological activity of said biological active agent is enhanced, and means for circulating said biological active agent between said primary bioreactor column and said rejuvenator column.

  5. Synthetic heparin-binding factor analogs

    DOE Patents [OSTI]

    Pena, Louis A.; Zamora, Paul O.; Lin, Xinhua; Glass, John D.

    2010-04-20

    The invention provides synthetic heparin-binding growth factor analogs having at least one peptide chain, and preferably two peptide chains branched from a dipeptide branch moiety composed of two trifunctional amino acid residues, which peptide chain or chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a linker, which may be a hydrophobic linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  6. Synthetic and Mechanistic Chemistry

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Mechanistic Chemistry Security at center of chemical and mechanistic chemistry research at Lab Project Description Los Alamos scientists are using synthetic and mechanistic chemistry to address energy security and other emerging challenges, including global security and core weapons missions. Synthetic chemistry includes work in ligands for catalysts, fission-products separations for isotope delivery, and labeled substrates for reaction mechanisms. Other research addresses materials

  7. Use of thermal analysis techniques (TG-DSC) for the characterization of diverse organic municipal waste streams to predict biological stability prior to land application

    SciTech Connect (OSTI)

    Fernandez, Jose M.; Plaza, Cesar; Polo, Alfredo; Plante, Alain F.

    2012-01-15

    ) techniques. Total amounts of CO{sub 2} respired indicated that the organic matter in the TS was the least stable, while that in the CS was the most stable. This was confirmed by changes detected with the spectroscopic methods in the composition of the organic wastes due to C mineralization. Differences were especially pronounced for TS, which showed a remarkable loss of aliphatic and proteinaceous compounds during the incubation process. TG, and especially DSC analysis, clearly reflected these differences between the three organic wastes before and after the incubation. Furthermore, the calculated energy density, which represents the energy available per unit of organic matter, showed a strong correlation with cumulative respiration. Results obtained support the hypothesis of a potential link between the thermal and biological stability of the studied organic materials, and consequently the ability of thermal analysis to characterize the maturity of municipal organic wastes and composts.

  8. Organic Matter Composition, Recycling Susceptibility, and the Effectiveness of the Biological Pump – An Evaluation Using NMR Spectra of Marine Plankton

    SciTech Connect (OSTI)

    Paytan, Adina

    2014-02-19

    Carbon (C) sequestration through fertilization of phytoplankton with micronutrients and enhancement of the absorption and retention of atmospheric C by ocean biota heavily depends on the efficiency of the “biological pump”. The long-term effectiveness of this strategy depends on a net transfer of C from the upper ocean-atmosphere system to the deep ocean where the C is removed from contact with the atmosphere for an extended period of time. This C removal can be equated to the amount of C fixation by phytoplankton minus the C cycling and regeneration in the euphotic zone. If the regeneration efficiency is increased, then despite increased C fixation, no net loss (sequestration) of C will result. A reduction in cycling efficiency in the euphotic zone, on the other hand, will increase the effectiveness of the “biological pump” and thus C sequestration. The degree of organic matter biodegradation and recycling depends on the “reactivity” of compounds synthesized by the biota, which in turn, is controlled by the structural characteristic of these compounds. There is considerable evidence that different phytoplankton taxa differ substantially in their biogeochemical characteristics and it is likely that the relative abundance of different compounds synthesized by these distinct taxa, and even within each group at different growth conditions, will differ too. This variability in biosynthesis and thus abundance of a wide range of organic compounds in the water column would lend itself to different susceptibility for biodegradation and regeneration. Knowledge of the distribution of various organic matter structural groups synthesized by distinct taxa, the dependence of the organic matter compound classes on different growth conditions (temperature, light, nutrients) and the selective susceptibility of these compound to regeneration is crucial for estimating the potential for rapid regeneration in the euphotic zone, and thus the effectiveness of the “biological

  9. Synthetic guide star generation

    DOE Patents [OSTI]

    Payne, Stephen A.; Page, Ralph H.; Ebbers, Christopher A.; Beach, Raymond J.

    2004-03-09

    A system for assisting in observing a celestial object and providing synthetic guide star generation. A lasing system provides radiation at a frequency at or near 938 nm and radiation at a frequency at or near 1583 nm. The lasing system includes a fiber laser operating between 880 nm and 960 nm and a fiber laser operating between 1524 nm and 1650 nm. A frequency-conversion system mixes the radiation and generates light at a frequency at or near 589 nm. A system directs the light at a frequency at or near 589 nm toward the celestial object and provides synthetic guide star generation.

  10. Synthetic guide star generation

    SciTech Connect (OSTI)

    Payne, Stephen A; Page, Ralph H; Ebbers, Christopher A; Beach, Raymond J

    2008-06-10

    A system for assisting in observing a celestial object and providing synthetic guide star generation. A lasing system provides radiation at a frequency at or near 938 nm and radiation at a frequency at or near 1583 nm. The lasing system includes a fiber laser operating between 880 nm and 960 nm and a fiber laser operating between 1524 nm and 1650 nm. A frequency-conversion system mixes the radiation and generates light at a frequency at or near 589 nm. A system directs the light at a frequency at or near 589 nm toward the celestial object and provides synthetic guide star generation.

  11. Biodegradable synthetic bone composites

    DOE Patents [OSTI]

    Liu, Gao; Zhao, Dacheng; Saiz, Eduardo; Tomsia, Antoni P.

    2013-01-01

    The invention provides for a biodegradable synthetic bone composition comprising a biodegradable hydrogel polymer scaffold comprising a plurality of hydrolytically unstable linkages, and an inorganic component; such as a biodegradable poly(hydroxyethylmethacrylate)/hydroxyapatite (pHEMA/HA) hydrogel composite possessing mineral content approximately that of human bone.

  12. (Biological dosimetry)

    SciTech Connect (OSTI)

    Sega, G.A.

    1990-11-06

    The traveler participated in an International Symposium on Trends in Biological Dosimetry and presented an invited paper entitled, Adducts in sperm protamine and DNA vs mutation frequency.'' The purpose of the Symposium was to examine the applicability of new methods to study quantitatively the effects of xenobiotic agents (radiation and chemicals) on molecular, cellular and organ systems, with special emphasis on human biological dosimetry. The general areas covered at the meeting included studies on parent compounds and metabolites; protein adducts; DNA adducts; gene mutations; cytogenetic end-points and reproductive methods.

  13. Synthetic and Mechanistic Chemistry publications

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Synthetic and Mechanistic Chemistry » Synthetic and Mechanistic Synthetic and Mechanistic publications Research into alternative forms of energy, especially energy security, is one of the major national security imperatives of this century. Get Expertise Dave Thorn Chemistry Program Manager Email Josh Smith Chemistry Communications Email "Research into alternative forms of energy, of which biofuels is a key component, is one of the major national security imperatives of this century.

  14. (Biological dosimetry)

    SciTech Connect (OSTI)

    Preston, R.J.

    1990-12-17

    The traveler attended the 1st International Conference on Biological Dosimetry in Madrid, Spain. This conference was organized to provide information to a general audience of biologists, physicists, radiotherapists, industrial hygiene personnel and individuals from related fields on the current ability of cytogenetic analysis to provide estimates of radiation dose in cases of occupational or environmental exposure. There is a growing interest in Spain in biological dosimetry because of the increased use of radiation sources for medical and occupational uses, and with this the anticipated and actual increase in numbers of overexposure. The traveler delivered the introductory lecture on Biological Dosimetry: Mechanistic Concepts'' that was intended to provide a framework by which the more applied lectures could be interpreted in a mechanistic way. A second component of the trip was to provide advice with regard to several recent cases of overexposure that had been or were being assessed by the Radiopathology and Radiotherapy Department of the Hospital General Gregorio Maranon'' in Madrid. The traveler had provided information on several of these, and had analyzed cells from some exposed or purportedly exposed individuals. The members of the biological dosimetry group were referred to individuals at REACTS at Oak Ridge Associated Universities for advice on follow-up treatment.

  15. Synthetic Design Microorganisms for Lignin Fuels and Chemicals Presentation for BETO 2015 Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic Design Microorganisms for Lignin Fuels and Chemicals 3/26/2015 Synthetic Biology Joshua S. Yuan Associate Professor and Director Texas A&M University This presentation does contain proprietary information 1 Project Goal: Design of Microorganisms for Lignin Fuel * The proposed research aims to address one of the most challenging issues in biofuel production: the utilization of lignin for fungible fuels. * Project Outcome: A viable biological platform for conversion of lignin into

  16. Biological production of organic compounds

    DOE Patents [OSTI]

    Yu, Jianping; Paddock, Troy; Carrieri, Damian; Maness, Pin-Ching; Seibert, Michael

    2016-04-12

    Strains of cyanobacteria that produce high levels of alpha ketoglutarate (AKG) and pyruvate are disclosed herein. Methods of culturing these cyanobacteria to produce AKG or pyruvate and recover AKG or pyruvate from the culture are also described herein. Nucleic acid sequences encoding polypeptides that function as ethylene-forming enzymes and their use in the production of ethylene are further disclosed herein. These nucleic acids may be expressed in hosts such as cyanobacteria, which in turn may be cultured to produce ethylene.

  17. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O.; Pena, Louis A.; Lin, Xinhua

    2009-10-06

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  18. Dual chain synthetic heparin-binding growth factor analogs

    DOE Patents [OSTI]

    Zamora, Paul O.; Pena, Louis A.; Lin, Xinhua

    2012-04-24

    The invention provides synthetic heparin-binding growth factor analogs having two peptide chains each branched from a branch moiety, such as trifunctional amino acid residues, the branch moieties separated by a first linker of from 3 to about 20 backbone atoms, which peptide chains bind a heparin-binding growth factor receptor and are covalently bound to a non-signaling peptide that includes a heparin-binding domain, preferably by a second linker, which may be a hydrophobic second linker. The synthetic heparin-binding growth factor analogs are useful as pharmaceutical agents, soluble biologics or as surface coatings for medical devices.

  19. SYNTHETIC SLING FAILURE - EVALUATIONS & RECOMMENDATIONS

    SciTech Connect (OSTI)

    MACKEY TC; HENDERSON CS

    2009-10-26

    The information and evaluations provided in this report were compiled to address the recurring problem of synthetic sling failure. As safety is the number one priority in all work aspects, a solution must be devised to prevent accidents from occurring. A total of thirteen cases regarding synthetic sling failure were evaluated in order to determine their causes, effects, and preventative measures. From the collected data, it was found that all cases in which the synthetic sling contacted the edge of its load resulted in sling failure. It is required that adequate synthetic sling protection devices be used to protect slings in any lift where the sling comes in direct contact with the edge or corner of its load. However, there are no consensus codes or standards stating the type, material, or purpose of the type of protective device used to protect the sling from being cut. Numerous industry standards and codes provide vague descriptions on how to protect synthetic slings. Without a clear, concise statement of how to protect synthetic slings, it is common for inadequate materials and sling protection devices to be used in an attempt to meet the intent of these requirements. The use of an inadequate sling protection device is the main cause of synthetic sling failure in all researched cases. Commercial sling protection devices come in many shapes and sizes, and have a variety of names, as well as advertised uses. 'Abrasion pads' and 'wear protectors' are two different names for products with the same intended purpose. There is no distinguishable way to determine the extent of sling protection which these devices will provide, or what specific scenarios they are made for. This creates room for error in a field where error is unacceptable. This report provides a recommended action for hoisting and rigging activities which require synthetic slings to contact a load, as well as recommended changes to industry standards which will benefit overall industry safety.

  20. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology LANL leads the world in computational finishing of microbial genomes Read caption + In 2013, Los Alamos scientist Richard Sayre and his team genetically modified the organisms to harvest light more efficiently for maximum production. Overview of Research and Highlights Researchers at Los Alamos National Laboratory are using their renowned expertise in genomics, computation, and experimental biology as the foundation of a dynamic systems biology capability. Systems

  1. Systems-Level Synthetic Biology for Advanced Biofuel Production

    SciTech Connect (OSTI)

    Ruffing, Anne; Jensen, Travis J.; Strickland, Lucas Marshall; Meserole, Stephen; Tallant, David

    2015-03-01

    Cyanobacteria have been shown to be capable of producing a variety of advanced biofuels; however, product yields remain well below those necessary for large scale production. New genetic tools and high throughput metabolic engineering techniques are needed to optimize cyanobacterial metabolisms for enhanced biofuel production. Towards this goal, this project advances the development of a multiple promoter replacement technique for systems-level optimization of gene expression in a model cyanobacterial host: Synechococcus sp. PCC 7002. To realize this multiple-target approach, key capabilities were developed, including a high throughput detection method for advanced biofuels, enhanced transformation efficiency, and genetic tools for Synechococcus sp. PCC 7002. Moreover, several additional obstacles were identified for realization of this multiple promoter replacement technique. The techniques and tools developed in this project will help to enable future efforts in the advancement of cyanobacterial biofuels.

  2. Synthetic Biology Offers New Hope For Malaria Victims

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    drugs. By adding new genes and engineering a new metabolic pathway in Escherichia coli bacteria, the researchers can quickly and cheaply synthesize a precursor to the...

  3. Differential Optical Synthetic Aperture Radar

    DOE Patents [OSTI]

    Stappaerts, Eddy A. (San Ramon, CA)

    2005-04-12

    A new differential technique for forming optical images using a synthetic aperture is introduced. This differential technique utilizes a single aperture to obtain unique (N) phases that can be processed to produce a synthetic aperture image at points along a trajectory. This is accomplished by dividing the aperture into two equal "subapertures", each having a width that is less than the actual aperture, along the direction of flight. As the platform flies along a given trajectory, a source illuminates objects and the two subapertures are configured to collect return signals. The techniques of the invention is designed to cancel common-mode errors, trajectory deviations from a straight line, and laser phase noise to provide the set of resultant (N) phases that can produce an image having a spatial resolution corresponding to a synthetic aperture.

  4. 7th Annual Systems Biology Symposium: Systems Biology and Engineering

    SciTech Connect (OSTI)

    Galitski, Timothy P.

    2008-04-01

    Systems biology recognizes the complex multi-scale organization of biological systems, from molecules to ecosystems. The International Symposium on Systems Biology has been hosted by the Institute for Systems Biology in Seattle, Washington, since 2002. The annual two-day event gathers the most influential researchers transforming biology into an integrative discipline investingating complex systems. Engineering and application of new technology is a central element of systems biology. Genome-scale, or very small-scale, biological questions drive the enigneering of new technologies, which enable new modes of experimentation and computational analysis, leading to new biological insights and questions. Concepts and analytical methods in engineering are now finding direct applications in biology. Therefore, the 2008 Symposium, funded in partnership with the Department of Energy, featured global leaders in "Systems Biology and Engineering."

  5. Synthetic substrates for enzyme analysis

    DOE Patents [OSTI]

    Bissell, E.R.; Mitchell, A.R.; Pearson, K.W.; Smith, R.E.

    1983-06-14

    Synthetic substrates are provided which may be represented as A-D. The A moiety includes an amino acid, polypeptide, or derivative. The D moiety includes 7-amino coumarin derivatives having an electron withdrawing substituent group at the 3 position carbon or fused between the 3 and 4 position carbons. No Drawings

  6. Synthetic substrates for enzyme analysis

    DOE Patents [OSTI]

    Bissell, Eugene R.; Mitchell, Alexander R.; Pearson, Karen W.; Smith, Robert E.

    1983-01-01

    Synthetic substrates are provided which may be represented as A-D. The A moiety thereof includes an amino acid, polypeptide, or derivative thereof. The D moiety thereof includes 7-amino coumarin derivatives having an electron withdrawing substituent group at the 3 position carbon or fused between the 3 and 4 position carbons.

  7. Synthetic LDL as targeted drug delivery vehicle

    DOE Patents [OSTI]

    Forte, Trudy M.; Nikanjam, Mina

    2012-08-28

    The present invention provides a synthetic LDL nanoparticle comprising a lipid moiety and a synthetic chimeric peptide so as to be capable of binding the LDL receptor. The synthetic LDL nanoparticle of the present invention is capable of incorporating and targeting therapeutics to cells expressing the LDL receptor for diseases associated with the expression of the LDL receptor such as central nervous system diseases. The invention further provides methods of using such synthetic LDL nanoparticles.

  8. Synthetic thermoelectric materials comprising phononic crystals

    DOE Patents [OSTI]

    El-Kady, Ihab F; Olsson, Roy H; Hopkins, Patrick; Reinke, Charles; Kim, Bongsang

    2013-08-13

    Synthetic thermoelectric materials comprising phononic crystals can simultaneously have a large Seebeck coefficient, high electrical conductivity, and low thermal conductivity. Such synthetic thermoelectric materials can enable improved thermoelectric devices, such as thermoelectric generators and coolers, with improved performance. Such synthetic thermoelectric materials and devices can be fabricated using techniques that are compatible with standard microelectronics.

  9. Biological response modifiers

    SciTech Connect (OSTI)

    Weller, R.E.

    1991-10-01

    Much of what used to be called immunotherapy is now included in the term biological response modifiers. Biological response modifiers (BRMs) are defined as those agents or approaches that modify the relationship between the tumor and host by modifying the host's biological response to tumor cells with resultant therapeutic effects.'' Most of the early work with BRMs centered around observations of spontaneous tumor regression and the association of tumor regression with concurrent bacterial infections. The BRM can modify the host response in the following ways: Increase the host's antitumor responses through augmentation and/or restoration of effector mechanisms or mediators of the host's defense or decrease the deleterious component by the host's reaction; Increase the host's defenses by the administration of natural biologics (or the synthetic derivatives thereof) as effectors or mediators of an antitumor response; Augment the host's response to modified tumor cells or vaccines, which might stimulate a greater response by the host or increase tumor-cell sensitivity to an existing response; Decrease the transformation and/or increase differentiation (maturation) of tumor cells; or Increase the ability of the host to tolerate damage by cytotoxic modalities of cancer treatment.

  10. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee R. Todd Anderson Program Manager, BER Climate and Environmental Sciences Anjuli Barnzai Program Manager, BER Climate and Environmental Sciences Susan Gregurick Program Manager, BER Biological Systems Yukiko Sekine NERSC Program Manager, ASCR Kathy Yelick NERSC Director Francesca Verdier NERSC Department Head for Services Richard Gerber NERSC User Services Harvey Wasserman NERSC System Architecture Last edited: 2016-04-29 11:35:21

  11. Synthetic carbonaceous fuels and feedstocks

    DOE Patents [OSTI]

    Steinberg, Meyer

    1980-01-01

    This invention relates to the use of a three compartment electrolytic cell in the production of synthetic carbonaceous fuels and chemical feedstocks such as gasoline, methane and methanol by electrolyzing an aqueous sodium carbonate/bicarbonate solution, obtained from scrubbing atmospheric carbon dioxide with an aqueous sodium hydroxide solution, whereby the hydrogen generated at the cathode and the carbon dioxide liberated in the center compartment are combined thermocatalytically into methanol and gasoline blends. The oxygen generated at the anode is preferably vented into the atmosphere, and the regenerated sodium hydroxide produced at the cathode is reused for scrubbing the CO.sub.2 from the atmosphere.

  12. Interferometry with synthetic gauge fields

    SciTech Connect (OSTI)

    Anderson, Brandon M.; Taylor, Jacob M.; Galitski, Victor M.

    2011-03-15

    We propose a compact atom interferometry scheme for measuring weak, time-dependent accelerations. Our proposal uses an ensemble of dilute trapped bosons with two internal states that couple to a synthetic gauge field with opposite charges. The trapped gauge field couples spin to momentum to allow time-dependent accelerations to be continuously imparted on the internal states. We generalize this system to reduce noise and estimate the sensitivity of such a system to be S{approx}10{sup -7}(m/s{sup 2}/{radical}(Hz)).

  13. Environmental data energy technology characterizations: synthetic fuels

    SciTech Connect (OSTI)

    Not Available

    1980-04-01

    Environmental Data Energy Technology Characterizations are publications which are intended to provide policy analysts and technical analysts with basic environmental data associated with key energy technologies. This publication provides documentation on synthetic fuels (coal-derived and oil shale). The transformation of the energy in coal and oil shale into a more useful form is described in this publication in terms of major activity areas in the synthetic fuel cycles, that is, in terms of activities which produce either an energy product or a fuel leading to the production of an energy product in a different form. The activities discussed in this document are coal liquefaction, coal gasification, in-situ gasification, and oil shales. These activities represent both well-documented and advanced activity areas. The former activities are characterized in terms of actual operating data with allowance for future modification where appropriate. Emissions are assumed to conform to environmental standards. The advanced activity areas examined are those like coal liquefaction and in-situ retorting of oil shale. For these areas, data from pilot or demonstration plants were used where available; otherwise, engineering studies provided the data. The organization of the chapters in this volume is designed to support the tabular presentation in the summary volume. Each chapter begins with a brief description of the activity under consideration. The standard characteristics, size, availability, mode of functioning and place in the fuel cycle are presented. Next, major legislative and/or technological factors influencing the commercial operation of the activity are offered. Discussions of resources consumed, residuals produced, and economics follow. To aid in comparing and linking the different activity areas, data for each area are normalized to 10/sup 12/ Btu of energy output from the activity.

  14. Organization | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Organization Organization Organization

  15. Biological Safety

    Office of Energy Efficiency and Renewable Energy (EERE)

    The DOE's Biological Safety Program provides a forum for the exchange of best practices, lessons learned, and guidance in the area of biological safety. This content is supported by the Biosurety Executive Team. The Biosurety Executive Team is a DOE-chartered group. The DOE Office of Worker Safety and Health Policy provides administrative support for this group. The group identifies biological safety-related issues of concern to the DOE and pursues solutions to issues identified.

  16. Method of producing synthetic pitch

    DOE Patents [OSTI]

    Kennel, Elliot B.; Stansberry, Peter G.; Stiller, Alfred H.; Zondlo, John W.

    2012-07-24

    Embodiments of a method are described for modifying pitches, oils, tars, and binders by using these materials as solvents to extract organic chemicals from coal.

  17. Findings and recommendations of the advisory panel on synthetic fuels. Advisory panel on synthetic fuels. Report for the Committee on Science and Technology, US House of Representatives

    SciTech Connect (OSTI)

    Not Available

    1980-01-01

    In a report to the US House of Representatives Committee on Science and Technology, the Advisory Panel defines the most critical energy problem facing the US: obtaining a sufficient supply of liquid hydrocarbons for transportation fuel and for other applications where substitution would be difficult, costly, and time-consuming. Any substantial contribution from synthetic fuels must involve the use of coal, oil shale, and biomass, with the raw materials coming from as many different regions of the country as possible. The panel makes recommendations regarding (1) the emphasis of the Department of Energy's synthetic-fuel demonstration program, (2) implementation of a synthetic-fuel production program, and (3) mitigation of the environmental and socioeconomic impacts of synthetic-fuel production. The panel specifically maintains that federal assistance to commercial-scale projects should be available on a competitive basis to those organizations willing to take substantial marketing risks.

  18. Policy implications of a shift in Federal synthetic-fuel-demonstration responsibility

    SciTech Connect (OSTI)

    LeGassie, R W.A.

    1981-03-26

    The President's intention to shift the focus of synthetic fuels demonstration activities from DOE to the Synthetic Fuels Corporation (SFC) is discussed. The Demonstration Plant Program is described. The realignment will impact the organization, activities and mandate of the SFC. It will also impact the continuity of demonstration program efforts presently underway. Present contractual agreements between the government and industrial participants in synfuel development are described. Differences between DOE and SFC in project selection criteria, funding, priorities and restrictions is outlined. Legislative changes are not required to shift the demonstration responsibility to the Synthetic Fuels Corporation. (DMC)

  19. Synthetic Aperture Radar Persistent Scatterer Interferometry...

    Open Energy Info (EERE)

    NA, 2010 DOI Not Provided Check for DOI availability: http:crossref.org Online Internet link for Synthetic Aperture Radar Persistent Scatterer Interferometry (PSInSAR)...

  20. Synthetic Genomics Inc | Open Energy Information

    Open Energy Info (EERE)

    search Name: Synthetic Genomics Inc. Place: La Jolla, California Sector: Hydro, Hydrogen, Renewable Energy Product: California-based company planning to create new types of...

  1. Types of variation in the cell population are similar to those in the populations of organisms. Developmental biology of cardiac myocytes

    SciTech Connect (OSTI)

    Brodsky, V.Ya.

    1994-09-01

    We review the studies of polyploidy in the heart muscle and changes in the abundance of myocytes during ontogenesis. Variation in the number and ploidy of muscle cells has been observed in the normal heart muscle. The types of genomic variation are similar to populational variations of organisms, i.e., hereditary and modificational variation. In studies of changes of the myocyte genome, we have found that growth of the heart has some additional reserve capacity, which may be realized under abnormal conditions when the heart muscle undergoes hypertrophy. This additional reserve capacity depends on the level of polyploidy in the cells of the heart muscle which is established during early postnatal embryogenesis. 30 refs., 10 figs.

  2. National Laboratory] Basic Biological Sciences(59) Biological...

    Office of Scientific and Technical Information (OSTI)

    Achievements of structural genomics Terwilliger, Thomas C. Los Alamos National Laboratory Basic Biological Sciences(59) Biological Science Biological Science Abstract Not...

  3. Biological preconcentrator

    DOE Patents [OSTI]

    Manginell, Ronald P.; Bunker, Bruce C.; Huber, Dale L.

    2008-09-09

    A biological preconcentrator comprises a stimulus-responsive active film on a stimulus-producing microfabricated platform. The active film can comprise a thermally switchable polymer film that can be used to selectively absorb and desorb proteins from a protein mixture. The biological microfabricated platform can comprise a thin membrane suspended on a substrate with an integral resistive heater and/or thermoelectric cooler for thermal switching of the active polymer film disposed on the membrane. The active polymer film can comprise hydrogel-like polymers, such as poly(ethylene oxide) or poly(n-isopropylacrylamide), that are tethered to the membrane. The biological preconcentrator can be fabricated with semiconductor materials and technologies.

  4. Theoretical Biology and Biophysics

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Theoretical Biology and Biophysics Modeling biological systems and analysis and informatics of molecular and cellular biological data Mathematical BiologyImmunology Fundamental ...

  5. Effects of Mine Waste Contamination on Fish and Wildlife Habitat at Multiple Levels of Biological Organization in the Methow River, 2001-2002 Annual Report.

    SciTech Connect (OSTI)

    Peplow, Dan; Edmonds, Robert.

    2002-06-01

    Glycogen Storage disease is occurring. GSD IV is caused by either a deficiency or inactivation of the glycogen branching enzyme that results in the synthesis of an abnormal glycogen molecule that is insoluble and has decreased branch points and increased chain length. These results show that the effects of mine waste contaminants can be expressed at all levels of organization from molecular to ecosystem-level responses.

  6. Synthetic fossil fuel technologies: health problems and intersociety...

    Office of Scientific and Technical Information (OSTI)

    Conference: Synthetic fossil fuel technologies: health problems and intersociety cooperation Citation Details In-Document Search Title: Synthetic fossil fuel technologies: health ...

  7. Designer synthetic media for studying microbial-catalyzed biofuel...

    Office of Scientific and Technical Information (OSTI)

    Designer synthetic media for studying microbial-catalyzed biofuel production Citation Details In-Document Search Title: Designer synthetic media for studying microbial-catalyzed ...

  8. Synthetic CO.sub.2 acceptor

    DOE Patents [OSTI]

    Lancet, Michael S.; Curran, George P.

    1981-08-18

    A synthetic CO.sub.2 acceptor consisting essentially of at least one compound selected from the group consisting of calcium oxide and calcium carbonate supported in a refractory carrier matrix, the carrier having the general formula Ca.sub.5 (SiO.sub.4).sub.2 CO.sub.3. A method for producing the synthetic CO.sub.2 acceptor is also disclosed.

  9. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology LANL leads the world in computational finishing of microbial ... and experimental biology as the foundation of a dynamic systems biology capability. ...

  10. Elements in biological AMS

    SciTech Connect (OSTI)

    Vogel, J.S.; McAninch, J.; Freeman, S.

    1996-08-01

    AMS (Accelerator Mass Spectrometry) provides high detection sensitivity for isotopes whose half-lives are between 10 years and 100 million years. {sup 14}C is the most developed of such isotopes and is used in tracing natural and anthropogenic organic compounds in the Earth`s biosphere. Thirty-three elements in the main periodic table and 17 lanthanides or actinides have long lived isotopes, providing potential tracers for research in elemental biochemistry. Overlap of biologically interesting heavy elements and possible AMS tracers is discussed.

  11. Synthetic aperture radar capabilities in development

    SciTech Connect (OSTI)

    Miller, M.

    1994-11-15

    The Imaging and Detection Program (IDP) within the Laser Program is currently developing an X-band Synthetic Aperture Radar (SAR) to support the Joint US/UK Radar Ocean Imaging Program. The radar system will be mounted in the program`s Airborne Experimental Test-Bed (AETB), where the initial mission is to image ocean surfaces and better understand the physics of low grazing angle backscatter. The Synthetic Aperture Radar presentation will discuss its overall functionality and a brief discussion on the AETB`s capabilities. Vital subsystems including radar, computer, navigation, antenna stabilization, and SAR focusing algorithms will be examined in more detail.

  12. Newmark-Hall synthetic history development

    SciTech Connect (OSTI)

    Aramayo, G.A.

    1990-11-01

    The methodology used to develop synthetic acceleration time histories with spectral content that envelopes the Newmark-Hall spectra is described. Six acceleration time histories are developed for two conditions of foundation and 3 critical damping factors. The target spectra corresponds to the mediam centered probability level.

  13. Coal based synthetic fuel technology assessment guides

    SciTech Connect (OSTI)

    Not Available

    1981-09-01

    Seventeen synthetic fuel processes are described in detail and compared on a uniform basis. This work was supported by the Energy Information Administration for the purpose of technology assessment of the processes, their efficiency, the capitalized and operating cost of plants of similar size, possible constraints, possible siting problems, regional effects, pollution control, etc. (LTN)

  14. Immobilization of radioiodine in synthetic boracite

    DOE Patents [OSTI]

    Babad, H.; Strachan, D.M.

    1982-09-23

    A nuclear waste storage product is disclosed in which radioiodine is incorporated in a synthetic boracite. The boracite may be prepared by reacting a transition metal iodide with an alkali horate under mild hydrothermal conditions, drying the reaction product, and then hot pressing.

  15. 2010 Tetrapyrroles, Chemistry & Biology of Gordon Research Conference

    SciTech Connect (OSTI)

    Angela Wilks

    2010-07-30

    The objective of the Chemistry & Biology of Tetrapyrroles Gordon Conference is to bring together researchers from diverse disciplines that otherwise would not interact. By bringing biologists, chemists, engineers and clinicians with a common interest in tetrapyrroles the conference provides a forum for cross-disciplinary ideas and collaboration. The perspective provided by biologists, chemists, and clinicians working in fields such as newly discovered defects in human porphyrin metabolism, the myriad of strategies for light harvesting in photosynthetic organisms, novel tetrapyrroles that serve as auxiliary chromophores or enzyme cofactors, synthetic strategies in the design of novel tetrapyrrole scaffolds, and tetrapyrrole based cell signaling and regulatory systems, makes this conference unique in the field. Over the years the growing evidence for the role of tetrapyrroles and their reactive intermediates in cell signaling and regulation has been of increasing importance at this conference. The 2010 conference on Chemistry & Biology of Tetrapyrroles will focus on many of these new frontiers as outlined in the preliminary program listed. Speakers will emphasize unpublished results and new findings in the field. The oral sessions will be followed by the highly interactive afternoon poster sessions. The poster sessions provide all conferees with the opportunity to present their latest research and to exchange ideas in a more informal setting. As in the past, this opportunity will continue during the nightly social gathering that takes place in the poster hall following the evening lectures. All conferees are encouraged to submit and present posters. At the conference the best poster in the areas of biology, chemistry and medicine will be selected by a panel of previous conference chairs.

  16. Power generation with synthetic liquid fuels

    SciTech Connect (OSTI)

    Lebowitz, H.E.; Rovesti, W.C.; Schreiber, H.

    1984-06-01

    Tests performed burning H-Coal and Exxon Donor Solvent (EDS) coal liquids in a utility combustion turbine, and a test burning EDS in a utility boiler are described. The H-Coal was produced by Ashland Synthetic Fuels, Inc. The EDS was produced in a pilot plant by Exxon Corporation in Baytown, Texas. The test objectives, site preparation, and performance results are discussed for both tests. 8 references, 6 tables.

  17. Development of Standards for NanoSIMS Analyses of Biological Materials

    SciTech Connect (OSTI)

    Davission, M L; Weber, P K; Pett-Ridge, J; Singer, S

    2008-07-31

    NanoSIMS is a powerful analytical technique for investigating element distributions at the nanometer scale, but quantifying elemental abundances requires appropriate standards, which are not readily available for biological materials. Standards for trace element analyses have been extensively developed for secondary ion mass spectrometry (SIMS) in the semiconductor industry and in the geological sciences. The three primary approaches for generating standards for SIMS are: (1) ion implantation (2) using previously characterized natural materials, and (3) preparing synthetic substances. Ion implantation is a reliable method for generating trace element standards, but it is expensive, which limits investigation of the analytical issues discussed above. It also requires low background levels of the elements of interest. Finding or making standard materials has the potential to provide more flexibility than ion implantation, but realizing homogeneity at the nano-scale is in itself a significant challenge. In this study, we experiment with all three approaches, but with an emphasis toward synthetic organic polymers in order to reduce costs, increase flexibility, and achieve a wide dynamic concentration range. This emphasis serves to meet the major challenge for biological samples of identifying matrix matched, homogeneous material. Biological samples themselves are typically heterogeneous at the scale of microns to 100s of microns, and therefore they are poor SIMS standards. Therefore, we focused on identifying 'biological-like' materials--either natural or synthetic--that can be used for standards. The primary criterion is that the material be as compositionally similar to biological samples as possible (primarily C, H, O, and N). For natural material we adsorbed organic colloids consisting of peptidoglycan (i.e., amino sugars), activated charcoal, and humic acids. Experiments conducted with Si on peptidoglycan showed low affinity as SiO{sub 2}, yet its distribution in

  18. Plant Vascular Biology 2010

    SciTech Connect (OSTI)

    Ding, Biao

    2014-11-17

    This grant supported the Second International Conference on Plant Vascular Biology (PVB 2010) held July 24-28, 2010 on the campus of Ohio State University, Columbus, Ohio. Biao Ding (Ohio State University; OSU) and David Hannapel (Iowa State University; ISU) served as co-chairs of this conference. Biao Ding served as the local organizer. PVB is defined broadly here to include studies on the biogenesis, structure and function of transport systems in plants, under conditions of normal plant growth and development as well as of plant interactions with pathogens. The transport systems cover broadly the xylem, phloem, plasmodesmata and vascular cell membranes. The PVB concept has emerged in recent years to emphasize the integrative nature of the transport systems and approaches to investigate them.

  19. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Workshops & Summer Schools Summer Students Structural Molecular Biology Illuminating ... major experimental driver for structural biology research, serving the needs of a large ...

  20. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology Genomics and Systems Biology Los Alamos scientists perform research in functional genomics and structural genomics, and applications for such work ...

  1. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    cells, viruses), plant or soil samples (USDA quarantines), recombinant DNA, or blood-borne pathogen. Biological Use Authorization The great majority of biological work at...

  2. Synthetic Metabolic Pathways for Bioconversion of Lignin Derivatives to Biofuels Presentation for BETO Project Peer Review

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic Metabolic Pathways for Bioconversion of Lignin Derivatives to Biofuels WBS: 2.3.2.104 March 25, 2015 Technology Area Review: Biochemical Conversion Principal Investigator: Adam M. Guss Organization: Oak Ridge National Laboratory 2 Goal Statement * Goal: Develop microbial biocatalysts to convert lignin-rich streams into value-added products * Relevance: Adding value to the lignin fraction of plant biomass will improve the economics of biorefineries to enable a bioeconomy Fuels +

  3. Apparatus for synthetic imaging of an object

    DOE Patents [OSTI]

    Sheen, David M

    2015-01-20

    An apparatus for synthetic imaging of an object is disclosed. The apparatus includes a plurality of transmitter elements spaced apart by a first distance in a first column and a plurality of receiver elements spaced apart by a second distance in a second column. The first distance and the second distance are different. The plurality of transmitter elements is a non-integer multiple of the plurality of receiver elements, and the plurality of receiver elements is a non-integer multiple of the plurality of transmitter elements.

  4. Innovative regulatory approach for synthetic-based muds.

    SciTech Connect (OSTI)

    Veil, J. A.

    1998-10-22

    The oil and gas industry has historically used water-based muds (WBMs) and oil-based muds (OBMs) in offshore drilling operations. WBMs are less expensive and are widely used. Both the WBMs and the associated drill cuttings maybe discharged from the platform to the sea provided that U.S. Environmental Protection Agency (EPA) discharge limitations are met. In some wells, however, difficult drilling conditions may force a switch from a WBM to an OBM. Neither the OBM nor the associated drill cuttings may be discharged. The OBM is hauled to shore, where it is processed for reuse, while the associated cuttings are injected in a disposal well at the platform or hauled to shore to a disposal facility. Both of these options are expensive. Synthetic-based muds (SBMs) are drilling fluids that use synthetic organic chemicals as base fluids. SBMs were developed to replace OBMs in difficult drilling situations. SBMs are more expensive than OBMs; however, they have superior environmental properties that may permit the cuttings to be discharged on-site. Like OBMs, SBMs are hauled ashore for processing and reuse after the well is drilled. The existing national effluent limitations guidelines (ELGs) for the offshore industry do not include requirements for SBM-cuttings since SBMs were not commonly in use at the time the ELGs were adopted. In late 1997, EPA announced that it would modify the offshore ELGs to include requirements for discharges of cuttings drilled with SBMs. For the first time in the history of the ELG program, EPA is following an innovative presumptive rulemaking process that will lead to development of draft regulations in one year rather than the 4- to 6-year period usually needed. With direction from the federal government to stakeholders concerning information needs for the regulatory development the industry has established several working groups to collect new scientific information on SBMs. This paper describes the presumptive rulemaking process and summarizes

  5. Novel Aryne Chemistry in Organic Synthesis

    SciTech Connect (OSTI)

    Zhijian Liu

    2006-12-12

    Arynes are among the most intensively studied systems in chemistry. However, many aspects of the chemistry of these reactive intermediates are not well understood yet and their use as reagents in synthetic organic chemistry has been somewhat limited, due to the harsh conditions needed to generate arynes and the often uncontrolled reactivity exhibited by these species. Recently, o-silylaryl triflates, which can generate the corresponding arynes under very mild reaction conditions, have been found very useful in organic synthesis. This thesis describes several novel and useful methodologies by employing arynes, which generate from o-silylaryl triflates, in organic synthesis. An efficient, reliable method for the N-arylation of amines, sulfonamides and carbamates, and the O-arylation of phenols and carboxylic acids is described in Chapter 1. Amines, sulfonamides, phenols, and carboxylic acids are good nucleophiles, which can react with arynes generated from a-silylaryl triflates to afford the corresponding N- and O-arylated products in very high yields. The regioselectivity of unsymmetrical arynes has also been studied. A lot of useful, functional groups can tolerate our reaction conditions. Carbazoles and dibenzofurans are important heteroaromatic compounds, which have a variety of biological activities. A variety of substituted carbazoles and dibenzofwans are readily prepared in good to excellent yields starting with the corresponding o-iodoanilines or o-iodophenols and o-silylaryl triflates by a treatment with CsF, followed by a Pd-catalyzed cyclization, which overall provides a one-pot, two-step process. By using this methodology, the carbazole alkaloid mukonine has been concisely synthesized in a very good yield. Insertion of an aryne into a {sigma}-bond between a nucleophile and an electrophile (Nu-E) should potentially be a very beneficial process from the standpoint of organic synthesis. A variety of substituted ketones and sulfoxides have been synthesized in

  6. Synthetic magnetoelectric coupling in a nanocomposite multiferroic

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Jain, P.; Wang, Q.; Roldan, M.; Glavic, A.; Lauter, V.; Urban, C.; Bi, Z.; Ahmed, T.; Zhu, J.; Varela, M.; et al

    2015-03-13

    Given the paucity of single phase multiferroic materials (with large ferromagnetic moment), composite systems seem an attractive solution to realize magnetoelectric coupling between ferromagnetic and ferroelectric order parameters. Despite having antiferromagnetic order, BiFeO₃ (BFO) has nevertheless been a key material due to excellent ferroelectric properties at room temperature. We studied a superlattice composed of 8 repetitions of 6 unit cells of La₀.₇Sr₀.₃MnO₃ (LSMO) grown on 5 unit cells of BFO. Significant net uncompensated magnetization in BFO, an insulating superlattice, is demonstrated using polarized neutron reflectometry. Remarkably, the magnetization enables magnetic field to change the dielectric properties of the superlattice, whichmore » we cite as an example of synthetic magnetoelectric coupling. Importantly, controlled creation of magnetic moment in BFO is a much needed path toward design and implementation of integrated oxide devices for next generation magnetoelectric data storage platforms.« less

  7. Inversion of synthetic aperture radar interferograms for sources...

    Open Energy Info (EERE)

    Inversion of synthetic aperture radar interferograms for sources of production-related subsidence at the Dixie Valley geothermal field Jump to: navigation, search OpenEI Reference...

  8. Synthetic Information and Decision Informatics for Complex Systems...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Dynamics & Simulation Science Laboratory SALISHAN 2010 Tackling Big Data: HPC Approaches to Informatics Synthetic Information and Decision Informatics for Complex Socially-Coupled...

  9. A versatile synthetic route for the preparation of titanium metal...

    Office of Scientific and Technical Information (OSTI)

    to mention a general synthetic strategy for various kinds of Ti-MOFs in the literature. ... Sponsoring Org: DOE - BASIC ENERGY SCIENCES Country of Publication: United States Language...

  10. Intramolecular hydrogen bonding as a synthetic tool to induce...

    Office of Scientific and Technical Information (OSTI)

    hydrogen bonding as a synthetic tool to induce chemical selectivity in acid catalyzed porphyrin synthesis Citation Details In-Document Search Title: Intramolecular hydrogen bonding ...

  11. Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform

    SciTech Connect (OSTI)

    Poust, S; Hagen, A; Katz, L; Keasling, JD

    2014-12-01

    Engineering modular polyketide synthases (PKSs) has the potential to be an effective methodology to produce existing and novel chemicals. However, this potential has only just begun to be realized. We propose the adoption of an iterative design-build-test-learn paradigm to improve PKS engineering. We suggest methods to improve engineered PKS design by learning from laboratory-based selection; adoption of DNA design software and automation to build constructs and libraries more easily; tools for the expression of engineered proteins in a variety of heterologous hosts; and mass spectrometry-based high-throughput screening methods. Finally, lessons learned during iterations of the design-build-test-learn cycle can serve as a knowledge base for the development of a single retrosynthesis algorithm usable by both PKS experts and non-experts alike.

  12. Synthetic Biology for Advanced Fuels (Opening Keynote Address - 2010 JGI User Meeting)

    ScienceCinema (OSTI)

    Keasling, Jay

    2011-04-28

    Jay Keasling, CEO of the Joint BioEnergy Institute, delivers the opening keynote on March 24, 2010 at the 5th Annual DOE JGI User Meeting

  13. Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications

    SciTech Connect (OSTI)

    Torella, JP; Lienert, F; Boehm, CR; Chen, JH; Way, JC; Silver, PA

    2014-08-07

    Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts, and they hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these pathways and circuits have become more complex, and the increasing need for standardization and insulation of genetic parts has resulted in sequence redundancies-for example, repeated terminator and insulator sequences-that complicate recombination-based assembly. We and others have recently developed DNA assembly methods, which we refer to collectively as unique nucleotide sequence (UNS)-guided assembly, in which individual DNA parts are flanked with UNSs to facilitate the ordered, recombination-based assembly of repetitive sequences. Here we present a detailed protocol for UNS-guided assembly that enables researchers to convert multiple DNA parts into sequenced, correctly assembled constructs, or into high-quality combinatorial libraries in only 2-3 d. If the DNA parts must be generated from scratch, an additional 2-5 d are necessary. This protocol requires no specialized equipment and can easily be implemented by a student with experience in basic cloning techniques.

  14. Mesoporous materials derived from synthetic organo-clays as novel hydrodesulfurization catalysts

    SciTech Connect (OSTI)

    Carrado, K.A.; Marshall, C.L.; Brenner, J.R.

    1996-12-31

    Various pore size distributions are found for synthetic organo-clay complexes from which the organic portion has been removed via calcination. The clays are prepared by hydrothermal crystallization of gels containing silica, magnesium hydroxide, lithium fluoride, and an organic of choice. The organic serves to impart long-range structural order to the inorganic network that does not disappear upon its removal. Mesoporous materials are prepared from a host of organic modifiers. For example, pore diameters of 40-50{Angstrom} result from tetraethyl ammonium and celluloses, and polydimethyl diallyl ammonium imparts diameters of about 110{Angstrom} on average. These materials have begun to be explored as hydrodesulfurization (HDS) catalyst supports. Preliminary results show performance commensurate with commercial catalysts for the mesoporous materials when a model oil feed is used (1% dibenzothiophene in hexadecane). The target application is HDS of an actual heavy crude oil from California.

  15. Recent Advances on Carbon Nanospheres. Synthetic Routes and Applications

    SciTech Connect (OSTI)

    Zhang, Pengfei; Qiao, Zhenan; Dai, Sheng

    2015-04-02

    Carbon-based materials are the most popular material types in both fundamental research and industrial applications, partly because of their well-controlled nano-morphologies. In the past two decades, we have witnessed a number of breakthroughs in carbon research: fullerenes, carbon nanotubes, and more recently graphene. Nowadays, carbon nanospheres are attracting more and more attention worldwide due to their excellent performance in various fields: drug delivery, heterogeneous catalysis, encapsulation of support and electrode materials. Actually, spherical carbon is an old material, whereas controlling carbon spheres in the nanometer range is a recent story. In the past 5 years, it has become possible to precisely control the particle size, surface area, pore size, chemical composition, and dispersity of carbon nanospheres. Toward this end, a number of synthetic strategies are emerging, such as hydrothermal carbonization of biomass-based resources, extended Stöber synthesis, and organic–organic self-assembly via different binding methods. In this feature article, we summarize recent routes for carbon nanospheres and briefly touch on their applications to shed light on the potential of this field. Throughout this article, a special emphasis is placed on the possible modulation of spherical structures at the nanoscale, and we wish to inspire many more designs and applications of carbon nanostructures in the near future.

  16. Recent Advances on Carbon Nanospheres. Synthetic Routes and Applications

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Zhang, Pengfei; Qiao, Zhenan; Dai, Sheng

    2015-04-02

    Carbon-based materials are the most popular material types in both fundamental research and industrial applications, partly because of their well-controlled nano-morphologies. In the past two decades, we have witnessed a number of breakthroughs in carbon research: fullerenes, carbon nanotubes, and more recently graphene. Nowadays, carbon nanospheres are attracting more and more attention worldwide due to their excellent performance in various fields: drug delivery, heterogeneous catalysis, encapsulation of support and electrode materials. Actually, spherical carbon is an old material, whereas controlling carbon spheres in the nanometer range is a recent story. In the past 5 years, it has become possible tomore » precisely control the particle size, surface area, pore size, chemical composition, and dispersity of carbon nanospheres. Toward this end, a number of synthetic strategies are emerging, such as hydrothermal carbonization of biomass-based resources, extended Stöber synthesis, and organic–organic self-assembly via different binding methods. In this feature article, we summarize recent routes for carbon nanospheres and briefly touch on their applications to shed light on the potential of this field. Throughout this article, a special emphasis is placed on the possible modulation of spherical structures at the nanoscale, and we wish to inspire many more designs and applications of carbon nanostructures in the near future.« less

  17. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Molecular Biology Group hosted a 3-day comprehensive workshop on the use of non-crystalline small-angle x-ray scattering and diffraction techniques in structural biology research. ...

  18. Motion Measurement for Synthetic Aperture Radar.

    SciTech Connect (OSTI)

    Doerry, Armin W.

    2015-01-01

    Synthetic Aperture Radar (SAR) measures radar soundings from a set of locations typically along the flight path of a radar platform vehicle. Optimal focusing requires precise knowledge of the sounding source locations in 3 - D space with respect to the target scene. Even data driven focusing techniques (i.e. autofocus) requires some degree of initial fidelity in the measurements of the motion of the radar. These requirements may be quite stringent especially for fine resolution, long ranges, and low velocities. The principal instrument for measuring motion is typically an Inertial Measurement Unit (IMU), but these instruments have inherent limi ted precision and accuracy. The question is %22How good does an IMU need to be for a SAR across its performance space?%22 This report analytically relates IMU specifications to parametric requirements for SAR. - 4 - Acknowledgements Th e preparation of this report is the result of a n unfunded research and development activity . Although this report is an independent effort, it draws heavily from limited - release documentation generated under a CRADA with General Atomics - Aeronautical System, Inc. (GA - ASI), and under the Joint DoD/DOE Munitions Program Memorandum of Understanding. Sandia National Laboratories is a multi - program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of En ergy's National Nuclear Security Administration under contract DE - AC04 - 94AL85000.

  19. Semiconductor Nanocrystals for Biological Imaging

    SciTech Connect (OSTI)

    Fu, Aihua; Gu, Weiwei; Larabell, Carolyn; Alivisatos, A. Paul

    2005-06-28

    Conventional organic fluorophores suffer from poor photo stability, narrow absorption spectra and broad emission feature. Semiconductor nanocrystals, on the other hand, are highly photo-stable with broad absorption spectra and narrow size-tunable emission spectra. Recent advances in the synthesis of these materials have resulted in bright, sensitive, extremely photo-stable and biocompatible semiconductor fluorophores. Commercial availability facilitates their application in a variety of unprecedented biological experiments, including multiplexed cellular imaging, long-term in vitro and in vivo labeling, deep tissue structure mapping and single particle investigation of dynamic cellular processes. Semiconductor nanocrystals are one of the first examples of nanotechnology enabling a new class of biomedical applications.

  20. Computational optimization of synthetic water channels.

    SciTech Connect (OSTI)

    Rogers, David Michael; Rempe, Susan L. B.

    2012-12-01

    Membranes for liquid and gas separations and ion transport are critical to water purification, osmotic energy generation, fuel cells, batteries, supercapacitors, and catalysis. Often these membranes lack pore uniformity and robustness under operating conditions, which can lead to a decrease in performance. The lack of uniformity means that many pores are non-functional. Traditional membranes overcome these limitations by using thick membrane materials that impede transport and selectivity, which results in decreased performance and increased operating costs. For example, limitations in membrane performance demand high applied pressures to deionize water using reverse osmosis. In contrast, cellular membranes combine high flux and selective transport using membrane-bound protein channels operating at small pressure differences. Pore size and chemistry in the cellular channels is defined uniformly and with sub-nanometer precision through protein folding. The thickness of these cellular membranes is limited to that of the cellular membrane bilayer, about 4 nm thick, which enhances transport. Pores in the cellular membranes are robust under operating conditions in the body. Recent efforts to mimic cellular water channels for efficient water deionization produced a significant advance in membrane function. The novel biomimetic design achieved a 10-fold increase in membrane permeability to water flow compared to commercial membranes and still maintained high salt rejection. Despite this success, there is a lack of understanding about why this membrane performs so well. To address this lack of knowledge, we used highperformance computing to interrogate the structural and chemical environments experienced by water and electrolytes in the newly created biomimetic membranes. We also compared the solvation environments between the biomimetic membrane and cellular water channels. These results will help inform future efforts to optimize and tune the performance of synthetic

  1. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee R. Todd Anderson Program Manager, BER Climate and Environmental Sciences Anjuli Barnzai Program Manager, BER Climate and Environmental...

  2. Synthetic muscle experiment will likely return to Earth in March |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Princeton Plasma Physics Lab Synthetic muscle experiment will likely return to Earth in March By Jeanne Jackson DeVoe October 26, 2015 Tweet Widget Google Plus One Share on Facebook A photo taken by Astronaut Scott Kelly of a sample of the synthetic muscle experiment floating in the International Space Station. (Photo by Scott Kelly, Courtesy of NASA ) A photo taken by Astronaut Scott Kelly of a sample of the synthetic muscle experiment floating in the International Space Station. Gallery: A

  3. Biological tracer method

    DOE Patents [OSTI]

    Strong-Gunderson, J.M.; Palumbo, A.V.

    1998-09-15

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer. 2 figs.

  4. Biological tracer method

    DOE Patents [OSTI]

    Strong-Gunderson, Janet M. (Ten Mile, TN); Palumbo, Anthony V. (Oak Ridge, TN)

    1998-01-01

    The present invention is a biological tracer method for characterizing the movement of a material through a medium, comprising the steps of: introducing a biological tracer comprising a microorganism having ice nucleating activity into a medium; collecting at least one sample of the medium from a point removed from the introduction point; and analyzing the sample for the presence of the biological tracer. The present invention is also a method for using a biological tracer as a label for material identification by introducing a biological tracer having ice nucleating activity into a material, collecting a sample of a portion of the labelled material and analyzing the sample for the presence of the biological tracer.

  5. Genomics and Systems Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Genomics and Systems Biology Genomics and Systems Biology Los Alamos scientists perform research in functional genomics and structural genomics, and applications for such work cover diverse fields such as energy, agriculture, and environmental cleanup. Contact Us Babetta Marrone Biofuels Program Manager Email Cheryl Kuske DOE BER Biological System Science Division Program Manager Email Kirsten McCabe Emerging Threats Program Manager Email Rebecca McDonald Bioscience Communications Email "We

  6. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M.; McDowell, Andrew F.

    2015-11-24

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  7. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2013-02-26

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  8. Biological detector and method

    DOE Patents [OSTI]

    Sillerud, Laurel; Alam, Todd M; McDowell, Andrew F

    2014-04-15

    A biological detector includes a conduit for receiving a fluid containing one or more magnetic nanoparticle-labeled, biological objects to be detected and one or more permanent magnets or electromagnet for establishing a low magnetic field in which the conduit is disposed. A microcoil is disposed proximate the conduit for energization at a frequency that permits detection by NMR spectroscopy of whether the one or more magnetically-labeled biological objects is/are present in the fluid.

  9. Structural Molecular Biology, SSRL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Our Mission Our Mission The SSRL Structural Molecular Biology program operates as a integrated resource and has three primary areas (or cores) of technological research and ...

  10. Potential Additives to Promote Seal Swell in Synthetic Fuels...

    Office of Scientific and Technical Information (OSTI)

    Synthetic, fuels derived from the Fischer-Tropsch (F-T) process using natural gas or coal-derived synthesis gas as feedstocks can be used for powering ground vehicles, aircraft, ...

  11. Synthetic muscle developed with PPPL scientists' help ready for launch |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Princeton Plasma Physics Lab Synthetic muscle developed with PPPL scientists' help ready for launch By Jeanne Jackson DeVoe April 8, 2015 Tweet Widget Google Plus One Share on Facebook Gallery: The Dragon spacecraft in orbit. Photo courtesy of NASA) (Photo by Photo courtesy of NASA) The Dragon spacecraft in orbit. Photo courtesy of NASA) The synthetic muscle material before it was packed for transport to the International Space Station. ( Photo courtesy of NASA ) (Photo by Photo courtesy of

  12. Application of Synthetic Diesel Fuels | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Synthetic Diesel Fuels Application of Synthetic Diesel Fuels 2005 Diesel Engine Emissions Reduction (DEER) Conference Presentations and Posters 2005_deer_schaberg.pdf (462.75 KB) More Documents & Publications Effect of GTL Diesel Fuels on Emissions and Engine Performance The Potential of GTL Diesel to Meet Future Exhaust Emission Limits Performance Characteristics of Coal-to-Liquids (CTL) Diesel in a 50-State Emissions Compliant Passenger Car

  13. Intramolecular hydrogen bonding as a synthetic tool to induce chemical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    selectivity in acid catalyzed porphyrin synthesis Intramolecular hydrogen bonding as a synthetic tool to induce chemical selectivity in acid catalyzed porphyrin synthesis Authors: Megiatto, J. D., Patterson, D., Sherman, B. D., Moore, T. A., Gust, D., and Moore, A. L. Title: Intramolecular hydrogen bonding as a synthetic tool to induce chemical selectivity in acid catalyzed porphyrin synthesis Source: Chemical Communications Year: 2012 Volume: 48 Pages: 4558-4560 ABSTRACT: A straightforward

  14. Synthetic environment employing a craft for providing user perspective reference

    DOE Patents [OSTI]

    Maples, Creve; Peterson, Craig A.

    1997-10-21

    A multi-dimensional user oriented synthetic environment system allows application programs to be programmed and accessed with input/output device independent, generic functional commands which are a distillation of the actual functions performed by any application program. A shared memory structure allows the translation of device specific commands to device independent, generic functional commands. Complete flexibility of the mapping of synthetic environment data to the user is thereby allowed. Accordingly, synthetic environment data may be provided to the user on parallel user information processing channels allowing the subcognitive mind to act as a filter, eliminating irrelevant information and allowing the processing of increase amounts of data by the user. The user is further provided with a craft surrounding the user within the synthetic environment, which craft, imparts important visual referential an motion parallax cues, enabling the user to better appreciate distances and directions within the synthetic environment. Display of this craft in close proximity to the user's point of perspective may be accomplished without substantially degrading the image resolution of the displayed portions of the synthetic environment.

  15. Integrated Biological Control

    SciTech Connect (OSTI)

    JOHNSON, A.R.

    2002-09-01

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects; and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (apriori) or in response to existing contamination spread (aposteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and apriori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, aposteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  16. Integrated Biological Control

    SciTech Connect (OSTI)

    JOHNSON, A.R.

    2003-10-09

    Biological control is any activity taken to prevent, limit, clean up, or remediate potential environmental, health and safety, or workplace quality impacts from plants, animals, or microorganisms. At Hanford the principal emphasis of biological control is to prevent the transport of radioactive contamination by biological vectors (plants, animals, or microorganisms), and where necessary, control and clean up resulting contamination. Other aspects of biological control at Hanford include industrial weed control (e.g.; tumbleweeds), noxious weed control (invasive, non-native plant species), and pest control (undesirable animals such as rodents and stinging insects, and microorganisms such as molds that adversely affect the quality of the workplace environment). Biological control activities may be either preventive (a priori) or in response to existing contamination spread (a posteriori). Surveillance activities, including ground, vegetation, flying insect, and other surveys, and a priori control actions, such as herbicide spraying and placing biological barriers, are important in preventing radioactive contamination spread. If surveillance discovers that biological vectors have spread radioactive contamination, a posteriori control measures, such as fixing contamination, followed by cleanup and removal of the contamination to an approved disposal location are typical response functions. In some cases remediation following the contamination cleanup and removal is necessary. Biological control activities for industrial weeds, noxious weeds and pests have similar modes of prevention and response.

  17. Biological sample collector

    DOE Patents [OSTI]

    Murphy, Gloria A.

    2010-09-07

    A biological sample collector is adapted to a collect several biological samples in a plurality of filter wells. A biological sample collector may comprise a manifold plate for mounting a filter plate thereon, the filter plate having a plurality of filter wells therein; a hollow slider for engaging and positioning a tube that slides therethrough; and a slide case within which the hollow slider travels to allow the tube to be aligned with a selected filter well of the plurality of filter wells, wherein when the tube is aligned with the selected filter well, the tube is pushed through the hollow slider and into the selected filter well to sealingly engage the selected filter well and to allow the tube to deposit a biological sample onto a filter in the bottom of the selected filter well. The biological sample collector may be portable.

  18. Large Scale Computing and Storage Requirements for Biological and

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Environmental Research: Target 2014 Large Scale Computing and Storage Requirements for Biological and Environmental Research: Target 2014 BERFrontcover.png A BER / ASCR / NERSC Workshop May 7-8, 2009 Final Report Large Scale Computing and Storage Requirements for Biological and Environmental Research, Report of the Joint BER / NERSC Workshop Conducted May 7-8, 2009 Rockville, MD Goals This workshop was jointly organized by the Department of Energy's Office of Biological & Environmental

  19. Biological and Chemical Security

    SciTech Connect (OSTI)

    Fitch, P J

    2002-12-19

    The LLNL Chemical & Biological National Security Program (CBNP) provides science, technology and integrated systems for chemical and biological security. Our approach is to develop and field advanced strategies that dramatically improve the nation's capabilities to prevent, prepare for, detect, and respond to terrorist use of chemical or biological weapons. Recent events show the importance of civilian defense against terrorism. The 1995 nerve gas attack in Tokyo's subway served to catalyze and focus the early LLNL program on civilian counter terrorism. In the same year, LLNL began CBNP using Laboratory-Directed R&D investments and a focus on biodetection. The Nunn-Lugar-Domenici Defense Against Weapons of Mass Destruction Act, passed in 1996, initiated a number of U.S. nonproliferation and counter-terrorism programs including the DOE (now NNSA) Chemical and Biological Nonproliferation Program (also known as CBNP). In 2002, the Department of Homeland Security was formed. The NNSA CBNP and many of the LLNL CBNP activities are being transferred as the new Department becomes operational. LLNL has a long history in national security including nonproliferation of weapons of mass destruction. In biology, LLNL had a key role in starting and implementing the Human Genome Project and, more recently, the Microbial Genome Program. LLNL has over 1,000 scientists and engineers with relevant expertise in biology, chemistry, decontamination, instrumentation, microtechnologies, atmospheric modeling, and field experimentation. Over 150 LLNL scientists and engineers work full time on chemical and biological national security projects.

  20. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  1. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  2. Work with Biological Materials

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Work with Biological Materials Print Planning A complete Experiment Safety Sheet (ESS) is required before work can be done at the ALS. This ESS is either a part of the proposal process or may be completed as an independent document. In the ESS, identify each material (including all biological materials) with which you will be working. The regulatory oversight for biological work is very complicated and we need to understand the risk levels involved with the material you plan to use at the ALS,

  3. Biological and Environmental Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Science Biological Science The protozoan Plasmodium falciparum gliding through a cell in the gut of a mosquito, its primary host. Although five different species of Plasmodium can cause malaria, Plasmodium falciparum causes the most severe disease. | Photo courtesy of Wikipedia Commons. <a href="http://energy.gov/articles/malaria-researchers-find-weakness-global-killer">Read more</a> The protozoan Plasmodium falciparum gliding through a cell in the gut of a

  4. Biological and Environmental Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological and Environmental Research Biological and Environmental Research Understanding how genomic information is translated to functional capabilities, and the roles of Earth's biogeochemical systems so we can predict climate decades or centuries into the future. Get Expertise Cheryl Kuske (505) 665-4800 Email James Bossert (505) 667-3644 Email Manvendra Dubey (505) 665-3128 Email Kim Nitschke (505) 667-1186 Email Phil Jones (505) 667-6387 Email Cathy Wilson (505) 667-0202 Email Conducting

  5. Luminescent metal-organic frameworks (MOFs); a nanolaboratory for

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    photophysics | MIT-Harvard Center for Excitonics Luminescent metal-organic frameworks (MOFs); a nanolaboratory for photophysics February 7, 2012 at 3pm/36-428 Mark Allendorf Sandia National Laboratories allendorf001_000 Abstract: Metal-organic frameworks (MOFs) are a diverse class of crystalline supramolecular materials characterized by rigid, nanoporous structures and an exceptional level of synthetic versatility. Since the organic component of MOFs, referred to as the "linker,"

  6. Apparatus, systems, and methods for ultrasound synthetic aperature focusing

    DOE Patents [OSTI]

    Schuster, George J.; Crawford, Susan L.; Doctor, Steven R.; Harris, Robert V.

    2005-04-12

    One form of the present invention is a technique for interrogating a sample with ultrasound which includes: generating ultrasonic energy data corresponding to a volume of a sample and performing a synthetic aperture focusing technique on the ultrasonic energy data. The synthetic aperture focusing technique includes: defining a number of hyperbolic surfaces which extend through the volume at different depths and a corresponding number of multiple element accumulation vectors, performing a focused element calculation procedure for a group of vectors which are representative of the interior of a designated aperture, performing another focused element calculation procedure for vectors corresponding to the boundary of the aperture, and providing an image corresponding to features of the sample in accordance with the synthetic aperture focusing technique.

  7. Designer synthetic media for studying microbial-catalyzed biofuel production

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tang, Xiaoyu; da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir; Chambliss, Charles; Lau, Ming W; Xiao, Zeyi; Dale, Bruce E; Balan, Venkatesh

    2015-01-01

    Background: The fermentation inhibition of yeast or bacteria by lignocellulose-derived degradation products, during hexose/pentose co-fermentation, is a major bottleneck for cost-effective lignocellulosic biorefineries. To engineer microbial strains for improved performance, it is critical to understand the mechanisms of inhibition that affect fermentative organisms in the presence of major components of a lignocellulosic hydrolysate. The development of a synthetic lignocellulosic hydrolysate (SH) media with a composition similar to the actual biomass hydrolysate will be an important advancement to facilitate these studies. In this work, we characterized the nutrients and plant-derived decomposition products present in AFEX™ pretreated corn stover hydrolysate (ACH). Themore » SH was formulated based on the ACH composition and was further used to evaluate the inhibitory effects of various families of decomposition products during Saccharomyces cerevisiae 424A (LNH-ST) fermentation. Results: The ACH contained high levels of nitrogenous compounds, notably amides, pyrazines, and imidazoles. In contrast, a relatively low content of furans and aromatic and aliphatic acids were found in the ACH. Though most of the families of decomposition products were inhibitory to xylose fermentation, due to their abundance, the nitrogenous compounds showed the most inhibition. From these compounds, amides (products of the ammonolysis reaction) contributed the most to the reduction of the fermentation performance. However, this result is associated to a concentration effect, as the corresponding carboxylic acids (products of hydrolysis) promoted greater inhibition when present at the same molar concentration as the amides. Due to its complexity, the formulated SH did not perfectly match the fermentation profile of the actual hydrolysate, especially the growth curve. However, the SH formulation was effective for studying the inhibitory effect of various compounds on yeast

  8. Designer synthetic media for studying microbial-catalyzed biofuel production

    SciTech Connect (OSTI)

    Tang, Xiaoyu [Biogas Inst. of Ministry of Agriculture, Chengdu (China); da Costa Sousa, Leonardo [Michigan State Univ., East Lansing, MI (United States); Jin, Mingjie [Michigan State Univ., East Lansing, MI (United States); Chundawat, Shishir [Michigan State Univ., East Lansing, MI (United States); State Univ. of New Jersey, Piscataway, NJ (United States); Chambliss, Charles [Baylor Univ., Waco, TX (United States); Lau, Ming W [Michigan State Univ., East Lansing, MI (United States); Xiao, Zeyi [Sichuan Univ., Chengdu (China); Dale, Bruce E [Michigan State Univ., East Lansing, MI (United States); Balan, Venkatesh [Michigan State Univ., East Lansing, MI (United States)

    2015-01-01

    Background: The fermentation inhibition of yeast or bacteria by lignocellulose-derived degradation products, during hexose/pentose co-fermentation, is a major bottleneck for cost-effective lignocellulosic biorefineries. To engineer microbial strains for improved performance, it is critical to understand the mechanisms of inhibition that affect fermentative organisms in the presence of major components of a lignocellulosic hydrolysate. The development of a synthetic lignocellulosic hydrolysate (SH) media with a composition similar to the actual biomass hydrolysate will be an important advancement to facilitate these studies. In this work, we characterized the nutrients and plant-derived decomposition products present in AFEX pretreated corn stover hydrolysate (ACH). The SH was formulated based on the ACH composition and was further used to evaluate the inhibitory effects of various families of decomposition products during Saccharomyces cerevisiae 424A (LNH-ST) fermentation. Results: The ACH contained high levels of nitrogenous compounds, notably amides, pyrazines, and imidazoles. In contrast, a relatively low content of furans and aromatic and aliphatic acids were found in the ACH. Though most of the families of decomposition products were inhibitory to xylose fermentation, due to their abundance, the nitrogenous compounds showed the most inhibition. From these compounds, amides (products of the ammonolysis reaction) contributed the most to the reduction of the fermentation performance. However, this result is associated to a concentration effect, as the corresponding carboxylic acids (products of hydrolysis) promoted greater inhibition when present at the same molar concentration as the amides. Due to its complexity, the formulated SH did not perfectly match the fermentation profile of the actual hydrolysate, especially the growth curve. However, the SH formulation was effective for studying the inhibitory effect of various compounds on yeast fermentation

  9. 2013 METALS IN BIOLOGY GORDON RESEARCH CONFERENCE, JANUARY 20-25, 2013

    SciTech Connect (OSTI)

    Rosenzweig, Amy

    2013-01-25

    Typical topics for lectures and posters include: biochemical and biophysical characterization of new metal containing proteins, enzymes, nucleic acids, factors, and chelators from all forms of life; synthesis, detailed characterization, and reaction chemistry of biomimetic compounds; novel crystal and solution structures of biological molecules and synthetic metal-chelates; discussions of the roles that metals play in medicine, maintenance of the environment, and biogeochemical processes; metal homeostasis; application of theory and computations to the structure and mechanism of metal-containing biological systems; and novel applications of spectroscopy to metals in biological systems.

  10. Biological Effects of Ionizing Radiation

    DOE R&D Accomplishments [OSTI]

    Ingram, M.; Mason, W. B.; Whipple, G. H.; Howland, J. W.

    1952-04-07

    This report presents a review of present knowledge and concepts of the biological effects of ionizing radiations. Among the topics discussed are the physical and chemical effects of ionizing radiation on biological systems, morphological and physiological changes observed in biological systems subjected to ionizing radiations, physiological changes in the intact animal, latent changes following exposure of biological systems to ionizing radiations, factors influencing the biological response to ionizing radiation, relative effects of various ionizing radiations, and biological dosimetry.

  11. Green Biologics | Open Energy Information

    Open Energy Info (EERE)

    Biologics Jump to: navigation, search Name: Green Biologics Place: Oxfordshire, United Kingdom Sector: Biomass, Renewable Energy Product: Oxfordshire-based industrial biotech...

  12. Kapitza problem for the magnetic moments of synthetic antiferromagnetic systems

    SciTech Connect (OSTI)

    Dzhezherya, Yu. I.; Demishev, K. O.; Korenivskii, V. N.

    2012-08-15

    The dynamics of magnetization in synthetic antiferromagnetic systems with the magnetic dipole coupling in a rapidly oscillating field has been examined. It has been revealed that the system can behave similar to the Kapitza pendulum. It has been shown that an alternating magnetic field can be efficiently used to control the magnetic state of a cell of a synthetic antiferromagnet. Analytical relations have been obtained between the parameters of such an antiferromagnet and an external magnetic field at which certain quasistationary states are implemented.

  13. The Intersection of Physics and Biology

    ScienceCinema (OSTI)

    Liphardt, Jan [University of California, Berkeley, California, United States

    2010-09-01

    In April 1953, Watson and Crick largely defined the program of 20th century biology: obtaining the blueprint of life encoded in the DNA. Fifty years later, in 2003, the sequencing of the human genome was completed. Like any major scientific breakthrough, the sequencing of the human genome raised many more questions than it answered. I'll brief you on some of the big open problems in cell and developmental biology, and I'll explain why approaches, tools, and ideas from the physical sciences are currently reshaping biological research. Super-resolution light microscopies are revealing the intricate spatial organization of cells, single-molecule methods show how molecular machines function, and new probes are clarifying the role of mechanical forces in cell and tissue function. At the same time, Physics stands to gain beautiful new problems in soft condensed matter, quantum mechanics, and non-equilibrium thermodynamics.

  14. Lab Organizations

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizations Lab Organizations National security depends on science and technology. The United States relies on Los Alamos National Laboratory for the best of both. No place on Earth pursues a broader array of world-class scientific endeavors. Los Alamos National Security, LLC Leadership Team Organization Chart PRINCIPAL ASSOCIATE DIRECTORATES Capital Projects, Larry Simmons Global Security, Terry Wallace Operations and Business, Craig Leasure Science, Technology, and Engineering, Alan Bishop

  15. The acquisition of dangerous biological materials :

    SciTech Connect (OSTI)

    Aceto, Donato Gonzalo; Astuto-Gribble, Lisa M.; Gaudioso, Jennifer M.

    2007-11-01

    Numerous terrorist organizations have openly expressed interest in producing and deploying biological weapons. However, a limiting factor for many terrorists has been the acquisition of dangerous biological agents, as evidenced by the very few successful instances of biological weapons use compared to the number of documented hoaxes. Biological agents vary greatly in their ability to cause loss of life and economic damage. Some agents, if released properly, can kill many people and cause an extensive number of secondary infections; other agents will sicken only a small number of people for a short period of time. Consequently, several biological agents can potentially be used to perpetrate a bioterrorism attack but few are likely capable of causing a high consequence event. It is crucial, from a US national security perspective, to more deeply understand the likelihood that terrorist organizations can acquire the range of these agents. Few studies have attempted to comprehensively compile the technical information directly relevant to the acquisition of dangerous bacteria, viruses and toxins. In this report, technical fact sheets were assembled for 46 potentially dangerous biological agents. Much of the information was taken from various research sources which could ultimately and significantly expedite and improve bioterrorism threat assessments. By systematically examining a number of specific agent characteristics included in these fact sheets, it may be possible to detect, target, and implement measures to thwart future terrorist acquisition attempts. In addition, the information in these fact sheets may be used as a tool to help laboratories gain a rudimentary understanding of how attractive a method laboratory theft is relative to other potential acquisition modes.

  16. Biological Air Emissions Control

    Office of Energy Efficiency and Renewable Energy (EERE)

    Air quality standards are becoming more stringent for the U.S. wood products industry. Emissions of volatile organic compounds (VOCs) and hazardous air pollutants (HAPs) (including methanol,...

  17. Materials derived from synthetic organo-clay complexes as novel hydrodesulfurization catalyst supports.

    SciTech Connect (OSTI)

    Carrado, K. A.; Marshall, C. L.; Brenner, J. R.; Song, K.; Chemistry

    1998-01-01

    A series of mesoporous synthetic organo-clay complexes has been prepared by hydrothermal crystallization of gels containing silica, magnesium hydroxide, lithium fluoride, and an organic of choice, followed by calcination to remove the organics. The organic serves to impart structural order to the inorganic network that does not disappear upon its removal. The choice of organic modifier can be used to control the pore structure of the resulting mesoporous materials. Pore size distributions appear in some cases to be related to the type of polymer packing upon clay formation in situ. These materials are being explored as Co Mo hydrodesulfurization (HDS) catalyst supports. Preliminary HDS results show performance commensurate with commercial catalysis for the mesoporous materials when a model heavy oil feed is used (1 wt% S as dibenzothiophene in hexadecane). Temperature programmed reduction experiments of used catalysts suggest a relationship between HDS activity and ease of reduction of the CoMo/clay catalysts. Reactivity of the CoMo clay also correlates with the percentage of mesopore volume remaining after reaction. Losses in mesopore volume are largely recouped by recalcination, suggesting that reversible coke is formed inside the pore structure of clays faster than inside conventional alumina.

  18. Synthetic aperture design for increased SAR image rate

    DOE Patents [OSTI]

    Bielek, Timothy P.; Thompson, Douglas G.; Walker, Bruce C.

    2009-03-03

    High resolution SAR images of a target scene at near video rates can be produced by using overlapped, but nevertheless, full-size synthetic apertures. The SAR images, which respectively correspond to the apertures, can be analyzed in sequence to permit detection of movement in the target scene.

  19. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee Large Scale Computing and Storage Requirements for Nuclear Physics May 26-27, 2011 Ted Barnes DOE Office of Nuclear Physics Yukiko Sekine NERSC Program Manager, DOE Office of Advanced Computational Research Kathy Yelick NERSC Director Richard Gerber NERSC User Services Harvey Wasserman NERSC User Services Last edited: 2016-04-29 11:35:21

  20. Fermilab | About | Organization | Fermilab Organization | Explanation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Symbols Line Organization: sectors, divisions, sections Line Organization Matrix Organization: centers, projects and programs utilizing resources spanning the entire...

  1. Organization | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    About Us » Organization Organization Organization OCIO Organizational Chart (Printable) News & Blog CIO Leadership Organization Contact Us

  2. Plant biology research and training for the 21st century

    SciTech Connect (OSTI)

    Kelly, K.

    1992-01-01

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  3. Plant biology research and training for the 21st century

    SciTech Connect (OSTI)

    Kelly, K.

    1992-12-31

    The committee was assembled in response to a request from the National Science Foundation (NSF), the US Department of Agriculture (USDA), and the US Department of Energy (DoE). The leadership of these agencies asked the National Academy of Sciences through the National Research Council (NRC) to assess the status of plant-science research in the United States in light of the opportunities arising from advances inother areas of biology. NRC was asked to suggest ways of accelerating the application of these new biologic concepts and tools to research in plant science with the aim of enhancing the acquisition of new knowledge about plants. The charge to the committee was to examine the following: Organizations, departments, and institutions conducting plant biology research; human resources involved in plant biology research; graduate training programs in plant biology; federal, state, and private sources of support for plant-biology research; the role of industry in conducting and supporting plant-biology research; the international status of US plant-biology research; and the relationship of plant biology to leading-edge research in biology.

  4. Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

    DOE Patents [OSTI]

    Simmons, Blake A.; Hill, Vincent R.; Fintschenko, Yolanda; Cummings, Eric B.

    2010-10-12

    Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.

  5. Concentration and separation of biological organisms by ultrafiltratio...

    Office of Scientific and Technical Information (OSTI)

    Disclosed is a method for monitoring sources of public water supply for a variety of ... when samples are collected for monitoring or outbreak investigations, an approach ...

  6. Recent progress in the synthesis of metal–organic frameworks

    SciTech Connect (OSTI)

    Sun, Yujia; Zhou, Hong -Cai

    2015-09-25

    Metal–organic frameworks (MOFs) have attracted considerable attention for various applications due to their tunable structure, porosity and functionality. In general, MOFs have been synthesized from isolated metal ions and organic linkers under hydrothermal or solvothermal conditions via one-spot reactions. The emerging precursor approach and kinetically tuned dimensional augmentation strategy add more diversity to this field. In addition, to speed up the crystallization process and create uniform crystals with reduced size, many alternative synthesis routes have been explored. Recent advances in microwave-assisted synthesis and electrochemical synthesis are presented in this review. In recent years, post-synthetic approaches have been shown to be powerful tools to synthesize MOFs with modified functionality, which cannot be attained via de novo synthesis. In this study, some current accomplishments of post-synthetic modification (PSM) based on covalent transformations and coordinative interactions as well as post-synthetic exchange (PSE) in robust MOFs are provided.

  7. Recent progress in the synthesis of metal–organic frameworks

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sun, Yujia; Zhou, Hong -Cai

    2015-09-25

    Metal–organic frameworks (MOFs) have attracted considerable attention for various applications due to their tunable structure, porosity and functionality. In general, MOFs have been synthesized from isolated metal ions and organic linkers under hydrothermal or solvothermal conditions via one-spot reactions. The emerging precursor approach and kinetically tuned dimensional augmentation strategy add more diversity to this field. In addition, to speed up the crystallization process and create uniform crystals with reduced size, many alternative synthesis routes have been explored. Recent advances in microwave-assisted synthesis and electrochemical synthesis are presented in this review. In recent years, post-synthetic approaches have been shown to bemore » powerful tools to synthesize MOFs with modified functionality, which cannot be attained via de novo synthesis. In this study, some current accomplishments of post-synthetic modification (PSM) based on covalent transformations and coordinative interactions as well as post-synthetic exchange (PSE) in robust MOFs are provided.« less

  8. Synthetic fuel aromaticity and staged combustion. First quarterly technical progress report, September 23-December 31, 1980

    SciTech Connect (OSTI)

    Levy, Arthur; Longanbach, James R.; Chan, Lisa K.

    1981-01-28

    Synthetic liquid fuels, otherwise referred to as synfuels or coal-derived liquids, are probably best characterized from a combustion-environmental point of view as low in hydrogen, low in sulfur, high in nitrogen, and high in aromatics. As a consequence two of the more critical problems in synfuel combustion are NO/sub x/ formation and soot formation (and polycyclic organic matter). This program is directed to these two issues. At first hand the solutions to burning synfuels high in aromatics and fuel-bound nitrogen are diametrically opposed, i.e., high temperature and excess air keep soot levels down, low temperatures and vitiated air keep nitrogen oxide levels down. Staged combustion however offers a logical solution to the above. This program separates and analyzes the synfuel combustion problem via its component parts and then puts them together again phenomenologically via the stage combustion process.

  9. Organic Superconductors

    SciTech Connect (OSTI)

    Charles Mielke

    2009-02-27

    Intense magnetic fields are an essential tool for understanding layered superconductors. Fundamental electronic properties of organic superconductors are revealed in intense (60 tesla) magnetic fields. Properties such as the topology of the Fermi surface and the nature of the superconducting order parameter are revealed. With modest maximum critical temperatures~13K the charge transfer salt organic superconductors prove to be incredibly valuable materials as their electronically clean nature and layered (highly anisotropic) structures yield insights to the high temperature superconductors. Observation of de Haas-van Alphen and Shubnikov-de Haas quantum oscillatory phenomena, magnetic field induced superconductivity and re-entrant superconductivity are some of the physical phenomena observed in the charge transfer organic superconductors. In this talk, I will discuss the nature of organic superconductors and give an overview of the generation of intense magnetic fields; from the 60 tesla millisecond duration to the extreme 1000 tesla microsecond pulsed magnetic fields.

  10. Synthetic Ecology of Microbes: Mathematical Models and Applications...

    Office of Scientific and Technical Information (OSTI)

    GrantContract Number: SC0012627 Type: Published Article Journal Name: Journal of Molecular Biology Additional Journal Information: Journal Volume: 428; Journal Issue: 5 PB; ...

  11. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee Large Scale Computing and Storage Requirements for Advanced Scientific Computing Research An ASCR / NERSC Workshop January 5-6, 2011 Dr. Karen Pao ASCR Yukiko Sekine NERSC Program Manager, ASCR Kathy Yelick NERSC Director Francesca Verdier NERSC Department Head for Services Richard Gerber NERSC User Services John Shalf NERSC Advanced Technologies Group Harvey Wasserman NERSC User Services Last edited: 2016-04-29 11:35:22

  12. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee Large Scale Computing and Storage Requirements for Fusion Energy Science August 3-4, 2010 Dr. John Mandrekas Advanced Fusion Simulations; FES HPC Allocations Yukiko Sekine NERSC Program Manager, ASCR Kathy Yelick NERSC Director Francesca Verdier NERSC Department Head for Services Richard Gerber NERSC User Services Alice Koniges NERSC Advanced Technologies Harvey Wasserman NERSC User Services Last edited: 2016-04-29 11:35:21

  13. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee Large Scale Computing and Storage Requirements for High Energy Physics November 12-13, 2009 Amber Boehnlein Division Scientist, Fermi National Accelerator Laboratory, on assignment to DOE Office of HEP. Glen Crawford Program Manager, Research and Technology Division, DOE Office of HEP. Yukiko Sekine NERSC Program Manager, ASCR Kathy Yelick NERSC Director Francesca Verdier NERSC Department Head for Services Richard Gerber NERSC User Services Harvey

  14. Organizing Committee

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organizing Committee Organizing Committee Large Scale Computing and Storage Requirements for Basic Energy Sciences An ASCR / BES / NERSC Workshop February 9-10, 2010 Jim Davenport Program Manager for Theoretical Condensed Material Physics Mark R. Pederson Program Manager for Theoretical and Computational Chemistry Nicholas B. Woodward Program Manager, Geosciences Research Program Yukiko Sekine NERSC Program Manager, ASCR Kathy Yelick NERSC Director Francesca Verdier NERSC Department Head for

  15. Biological particle identification apparatus

    DOE Patents [OSTI]

    Salzman, Gary C.; Gregg, Charles T.; Grace, W. Kevin; Hiebert, Richard D.

    1989-01-01

    An apparatus and method for making multiparameter light scattering measurements from suspensions of biological particles is described. Fourteen of the sixteen Mueller matrix elements describing the particles under investigation can be substantially individually determined as a function of scattering angle and probing radiations wavelength, eight elements simultaneously for each of two apparatus configurations using an apparatus which incluees, in its simplest form, two polarization modulators each operating at a chosen frequency, one polarizer, a source of monochromatic electromagnetic radiation, a detector sensitive to the wavelength of radiation employed, eight phase-sensitive detectors, and appropriate electronics. A database of known biological particle suspensions can be assembled, and unknown samples can be quickly identified once measurements are performed on it according to the teachings of the subject invention, and a comparison is made with the database.

  16. Investigations of biological processes in Austrian MBT plants

    SciTech Connect (OSTI)

    Tintner, J.; Smidt, E.; Boehm, K.; Binner, E.

    2010-10-15

    Mechanical biological treatment (MBT) of municipal solid waste (MSW) has become an important technology in waste management during the last decade. The paper compiles investigations of mechanical biological processes in Austrian MBT plants. Samples from all plants representing different stages of degradation were included in this study. The range of the relevant parameters characterizing the materials and their behavior, e.g. total organic carbon, total nitrogen, respiration activity and gas generation sum, was determined. The evolution of total carbon and nitrogen containing compounds was compared and related to process operation. The respiration activity decreases in most of the plants by about 90% of the initial values whereas the ammonium release is still ongoing at the end of the biological treatment. If the biogenic waste fraction is not separated, it favors humification in MBT materials that is not observed to such extent in MSW. The amount of organic carbon is about 15% dry matter at the end of the biological treatment.

  17. BIOLOGICAL IRRADIATION FACILITY

    DOE Patents [OSTI]

    McCorkle, W.H.; Cern, H.S.

    1962-04-24

    A facility for irradiating biological specimens with neutrons is described. It includes a reactor wherein the core is off center in a reflector. A high-exposure room is located outside the reactor on the side nearest the core while a low-exposure room is located on the opposite side. Means for converting thermal neutrons to fast neutrons are movably disposed between the reactor core and the high and low-exposure rooms. (AEC)

  18. Synthetic aperture integration (SAI) algorithm for SAR imaging

    DOE Patents [OSTI]

    Chambers, David H; Mast, Jeffrey E; Paglieroni, David W; Beer, N. Reginald

    2013-07-09

    A method and system for detecting the presence of subsurface objects within a medium is provided. In some embodiments, the imaging and detection system operates in a multistatic mode to collect radar return signals generated by an array of transceiver antenna pairs that is positioned across the surface and that travels down the surface. The imaging and detection system pre-processes the return signal to suppress certain undesirable effects. The imaging and detection system then generates synthetic aperture radar images from real aperture radar images generated from the pre-processed return signal. The imaging and detection system then post-processes the synthetic aperture radar images to improve detection of subsurface objects. The imaging and detection system identifies peaks in the energy levels of the post-processed image frame, which indicates the presence of a subsurface object.

  19. Report of The Structural Biology Subcommittee of The Biological and

    Office of Science (SC) Website

    Environmental Research Advisory Committee | U.S. DOE Office of Science (SC) Report of The Structural Biology Subcommittee of The Biological and Environmental Research Advisory Committee Biological and Environmental Research Advisory Committee (BERAC) BERAC Home Meetings Members Charges/Reports Current BERAC Charges Archive of BERAC Reports Charter .pdf file (135KB) BER Committees of Visitors Federal Advisory Committees BER Home Charges/Reports Report of The Structural Biology Subcommittee of

  20. Synthetic nanotubes lay foundation for new technology: Artificial pores

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    mimic key features of natural pores | Argonne National Laboratory Synthetic nanotubes lay foundation for new technology: Artificial pores mimic key features of natural pores By Tona Kunz * July 17, 2012 Tweet EmailPrint Scientists have overcome key design hurdles to expand the potential uses of nanopores and nanotubes. The creation of smart nanotubes with selective mass transport opens up a wider range of applications for water purification, chemical separation and fighting disease.

  1. Sandia National Laboratories: Pathfinder Airborne ISR and Synthetic

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Aperture Radar (SAR) Systems Pathfinder Airborne ISR Systems What is SAR? Areas of Expertise Images VideoSAR Publications Facebook Twitter YouTube Flickr RSS Pathfinder Airborne ISR Systems Pathfinder Airborne ISR and Synthetic Aperture Radar (SAR) Systems Tactical Eyes for the Warfighter Tactical Eyes for the Warfighter Actionable Intelligence for the Decision Maker Actionable Intelligence for the Decision Maker All Weather, Persistent, Optical Like All Weather, Persistent, Optical Like

  2. Synthetic Antimicrobial Oligomers Induce Composition-dependent Topological

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Transition in Membranes Synthetic Antimicrobial Oligomers Induce Composition-dependent Topological Transition in Membranes The development of bacterial resistance to conventional antibiotics is a major public health concern. For example, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE) and Staphylococcus aureus (VRSA) have emerged as common nosocomial (hospital-originating) infections. Circumvention of such resistance may be possi ble by emulating

  3. CO2 Removal using a Synthetic Analogue of Carbonic Anhydrase

    SciTech Connect (OSTI)

    Harry Cordatos

    2010-09-14

    Project attempts to develop a synthetic analogue for carbonic anhydrase and incorporate it in a membrane for separation of CO2 from coal power plant flue gas. Conference poster presents result of first 9 months of project progress including concept, basic system architecture and membrane properties target, results of molecular modeling for analogue - CO2 interaction, and next steps of testing analogue resistance to flue gas contaminants.

  4. Sandia National Laboratories: Synthetic Aperture Radar (SAR) Imagery

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Images Facebook Twitter YouTube Flickr RSS Pathfinder Airborne ISR Systems Synthetic Aperture Radar (SAR) Imagery The following is a selection of imagery available for your viewing, sorted by frequency band and/or program. (Note: Resolutions are for original images prior to downsampling for web viewing.) Images are available for public reproduction. Please credit Sandia using the following statement 'Courtesy of Sandia National Laboratories, Airborne ISR' Click thumbnails below to enlarge

  5. Pentavalent Uranium Chemistry - Synthetic Pursuit Of A Rare Oxidation State

    SciTech Connect (OSTI)

    Graves, Christopher R; Kiplinger, Jaqueline L

    2009-01-01

    This feature article presents a comprehensive overview of pentavalent uranium systems in non-aqueous solution with a focus on the various synthetic avenues employed to access this unusual and very important oxidation state. Selected characterization data and theoretical aspects are also included. The purpose is to provide a perspective on this rapidly evolving field and identify new possibilities for future developments in pentavalent uranium chemistry.

  6. Method and apparatus for removing heat from electronic devices using synthetic jets

    DOE Patents [OSTI]

    Sharma, Rajdeep; Weaver, Jr., Stanton Earl; Seeley, Charles Erklin; Arik, Mehmet; Icoz, Tunc; Wolfe, Jr., Charles Franklin; Utturkar, Yogen Vishwas

    2014-04-15

    An apparatus for removing heat comprises a heat sink having a cavity, and a synthetic jet stack comprising at least one synthetic jet mounted within the cavity. At least one rod and at least one engaging structure to provide a rigid positioning of the at least one synthetic jet with respect to the at least one rod. The synthetic jet comprises at least one orifice through which a fluid is ejected.

  7. Method and apparatus for removing heat from electronic devices using synthetic jets

    SciTech Connect (OSTI)

    Sharma, Rajdeep; Weaver, Stanton Earl; Seeley, Charles Erklin; Arik, Mehmet; Icoz, Tunc; Wolfe, Jr., Charles Franklin; Utturkar, Yogen Vishwas

    2015-11-24

    An apparatus for removing heat comprises a heat sink having a cavity, and a synthetic jet stack comprising at least one synthetic jet mounted within the cavity. At least one rod and at least one engaging structure to provide a rigid positioning of the at least one synthetic jet with respect to the at least one rod. The synthetic jet comprises at least one orifice through which a fluid is ejected.

  8. Method and apparatus for removing heat from electronic devices using synthetic jets

    SciTech Connect (OSTI)

    Sharma, Rajdeep; Weaver, Stanton Earl; Seeley, Charles Erklin; Arik, Mehmet; Icoz, Tunc; Wolfe Jr, Charles Franklin; Utturkar, Yogen Vishwas

    2015-12-01

    An apparatus for removing heat comprises a heat sink having a cavity, and a synthetic jet stack comprising at least one synthetic jet mounted within the cavity. At least one rod and at least one engaging structure to provide a rigid positioning of the at least one synthetic jet with respect to the at least one rod. The synthetic jet comprises at least one orifice through which a fluid is ejected.

  9. Guidelines to improve airport preparedness against chemical and biological terrorism.

    SciTech Connect (OSTI)

    Edwards, Donna M.; Price, Phillip N.; Gordon, Susanna P.; Gadgil, Ashok

    2005-05-01

    Guidelines to Improve Airport Preparedness Against Chemical and Biological Terrorism is a 100-page document that makes concrete recommendations on improving security and assessing vulnerable areas and helps its readers understand the nature of chemical and biological attacks. The report has been turned over to Airports Council International (ACI) and the American Association of Airport Executives (AAAE), two organizations that together represent the interests of thousands of airport personnel and facilities in the U.S. and around the world.

  10. Fermilab | About | Organization | Fermilab Organization

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Organization Fermilab Organization < Back to About Fermilab Fermilab Org Chart Accelerator Division Accelerator Physics Center CMS Center Core Computing Division ESH&Q FESS Finance Section LBNF Project Far-Site LBNF Project Near-Site LBNF Project Office LBNF Project LCLS-II Project Neutrino Division Office of Communication Office of Integrated Planning and Performance Management Office of Project Support Services Office of the CFO Office of the CIO Office of the CPO PIP-II Project PPD

  11. Organization Chart | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Organization Chart Organization Chart Organization Chart Printable PDF Mission Leadership

  12. Energy from biological processes

    SciTech Connect (OSTI)

    Not Available

    1980-07-01

    This assessment responds to a request by the Senate Committee on Commerce, Science, and Transportation for an evaluation of the energy potential of various sources of plant and animal matter (biomass). This report complements an earlier OTA report on the Application of Solar Technology to Today's Energy Needs in evaluating the major solar energy resources available to the United States. The findings also will serve as part of the material to be used in an upcoming OTA assessment of synthetic fuels for transportation. This volume presents analyses of prominent biomass issues, summaries of four biomass fuel cycles, a description of biomass' place in two plausible energy futures, and discussions of policy options for promoting energy from biomass. The four fuel cycles - wood, alcohol fuels, grasses and crop residues, and animal wastes - were chosen because of their near- to mid-term energy potential and because of the public interest in them. A second volume presents technical analyses of the resource base, conversion technologies, and end uses that provide a basis for the discussion in this volume. Also included in Volume II are various unconventional approaches to bioenergy production as well as the use of biomass to produce chemicals.

  13. Biological effectiveness of neutrons: Research needs

    SciTech Connect (OSTI)

    Casarett, G.W.; Braby, L.A.; Broerse, J.J.; Elkind, M.M.; Goodhead, D.T.; Oleinick, N.L.

    1994-02-01

    The goal of this report was to provide a conceptual plan for a research program that would provide a basis for determining more precisely the biological effectiveness of neutron radiation with emphasis on endpoints relevant to the protection of human health. This report presents the findings of the experts for seven particular categories of scientific information on neutron biological effectiveness. Chapter 2 examines the radiobiological mechanisms underlying the assumptions used to estimate human risk from neutrons and other radiations. Chapter 3 discusses the qualitative and quantitative models used to organize and evaluate experimental observations and to provide extrapolations where direct observations cannot be made. Chapter 4 discusses the physical principles governing the interaction of radiation with biological systems and the importance of accurate dosimetry in evaluating radiation risk and reducing the uncertainty in the biological data. Chapter 5 deals with the chemical and molecular changes underlying cellular responses and the LET dependence of these changes. Chapter 6, in turn, discusses those cellular and genetic changes which lead to mutation or neoplastic transformation. Chapters 7 and 8 examine deterministic and stochastic effects, respectively, and the data required for the prediction of such effects at different organizational levels and for the extrapolation from experimental results in animals to risks for man. Gaps and uncertainties in this data are examined relative to data required for establishing radiation protection standards for neutrons and procedures for the effective and safe use of neutron and other high-LET radiation therapy.

  14. Abengoa Mojave Final Biological Opinion

    Broader source: Energy.gov [DOE]

    Biological Opinion on Mojave Solar, LLC's Mojave Solar Project, San Bernardino County, California (8-8-11-F-3)

  15. Kinetic model for anaerobic digestion of biogas biological sludge

    SciTech Connect (OSTI)

    Pavlostathis, S.G.; Gossett, J.M.

    1986-10-01

    The principal objective of this study was the development and evaluation of a comprehensive kinetic model capable of predicting digester performance when fed biological sludge. Preliminary conversion mechanisms such as cell deaths, lysis, and hydrolysis responsible for rendering viable biological sludge organisms to available substrate were studied in depth. The results of this study indicate that hydrolysis of the dead, particulate biomass - primarily consisting of protein - is the slowest step, and therefore kinetically controls the overall process of an anaerobic digestion of biological sludge. A kinetic model was developed which could accurately describe digester performance and predict effluent quality.

  16. Electrochemical destruction of organic acids

    SciTech Connect (OSTI)

    Gendes, J.D.; Hartsough, D.; Super, J.D.

    1994-12-31

    An electrochemical process for removing organic acids from an aqueous waste stream has been characterized. Biological treatment of aqueous organic acid waste streams has been the typical means of degrading organic acids, and the resultant biosludge is landfilled. In the electrochemical approach, aqueous organic acids may be efficiently converted to useful fuel in a one or two electron process. The possible reactions occurring are outlined here. The electrolysis of the sodium salts of acetic, propionic, and butyric acids has been studied both as single component solutions and mixtures. The reaction products as well as relative rates of destruction of the acid salts were measured. The effect of experimental variables such as current density, temperature, and anode material on the current efficiency and product distribution was investigated. Electrode stability due to platinum corrosion was identified as the major limitation to the process.

  17. Systems Biology Knowledgebase for a New Era in Biology A Genomics:GTL Report from the May 2008 Workshop

    SciTech Connect (OSTI)

    Gregurick, S.; Fredrickson, J. K.; Stevens, R.

    2009-03-01

    Biology has entered a systems-science era with the goal to establish a predictive understanding of the mechanisms of cellular function and the interactions of biological systems with their environment and with each other. Vast amounts of data on the composition, physiology, and function of complex biological systems and their natural environments are emerging from new analytical technologies. Effectively exploiting these data requires developing a new generation of capabilities for analyzing and managing the information. By revealing the core principles and processes conserved in collective genomes across all biology and by enabling insights into the interplay between an organism's genotype and its environment, systems biology will allow scientific breakthroughs in our ability to project behaviors of natural systems and to manipulate and engineer managed systems. These breakthroughs will benefit Department of Energy (DOE) missions in energy security, climate protection, and environmental remediation.

  18. Monitoring Biological Activity at Geothermal Power Plants

    SciTech Connect (OSTI)

    Peter Pryfogle

    2005-09-01

    The economic impact of microbial growth in geothermal power plants has been estimated to be as high as $500,000 annually for a 100 MWe plant. Many methods are available to monitor biological activity at these facilities; however, very few plants have any on-line monitoring program in place. Metal coupon, selective culturing (MPN), total organic carbon (TOC), adenosine triphosphate (ATP), respirometry, phospholipid fatty acid (PLFA), and denaturing gradient gel electrophoresis (DGGE) characterizations have been conducted using water samples collected from geothermal plants located in California and Utah. In addition, the on-line performance of a commercial electrochemical monitor, the BIoGEORGE?, has been evaluated during extended deployments at geothermal facilities. This report provides a review of these techniques, presents data on their application from laboratory and field studies, and discusses their value in characterizing and monitoring biological activities at geothermal power plants.

  19. Method for producing and regenerating a synthetic CO.sub.2 acceptor

    DOE Patents [OSTI]

    Lancet, Michael S [Pittsburgh, PA; Curran, George P [Pittsburgh, PA; Gorin, Everett [San Rafael, CA

    1982-01-01

    A method for producing a synthetic CO.sub.2 acceptor by feeding a mixture of finely divided silica and at least one finely divided calcium compound selected from the group consisting of calcium oxide and calcium carbonate to a fluidized bed; operating the fluidized bed at suitable conditions to produce pellets of synthetic CO.sub.2 acceptor and recovering the pellets of synthetic CO.sub.2 acceptor from the fluidized bed. Optionally, spent synthetic CO.sub.2 acceptor can be charged to the fluidized bed to produce regenerated pellets of synthetic CO.sub.2 acceptor.

  20. Method for producing and regenerating a synthetic CO[sub 2] acceptor

    DOE Patents [OSTI]

    Lancet, M. S.; Curran, G. P.; Gorin, E.

    1982-05-18

    A method is described for producing a synthetic CO[sub 2] acceptor by feeding a mixture of finely divided silica and at least one finely divided calcium compound selected from the group consisting of calcium oxide and calcium carbonate to a fluidized bed; operating the fluidized bed at suitable conditions to produce pellets of synthetic CO[sub 2] acceptor and recovering the pellets of synthetic CO[sub 2] acceptor from the fluidized bed. Optionally, spent synthetic CO[sub 2] acceptor can be charged to the fluidized bed to produce regenerated pellets of synthetic CO[sub 2] acceptor. 1 fig.

  1. Neutron instrumentation for biology

    SciTech Connect (OSTI)

    Mason, S.A.

    1994-12-31

    In the October 1994 round of proposals at the ILL, the external biology review sub- committee was asked to allocate neutron beam time to a wide range of experiments, on almost half the total number of scheduled neutron instruments: on 3 diffractometers, on 3 small angle scattering instruments, and on some 6 inelastic scattering spectrometers. In the 3.5 years since the temporary reactor shutdown, the ILL`s management structure has been optimized, budgets and staff have been trimmed, the ILL reactor has been re-built, and many of the instruments up-graded, many powerful (mainly Unix) workstations have been introduced, and the neighboring European Synchrotron Radiation Facility has established itself as the leading synchrotron radiation source and has started its official user program. The ILL reactor remains the world`s most intense dedicated neutron source. In this challenging context, it is of interest to review briefly the park of ILL instruments used to study the structure and energetics of small and large biological systems. A brief summary will be made of each class of experiments actually proposed in the latest ILL proposal round.

  2. 2013 Biological Hydrogen Production Workshop Summary Report ...

    Energy Savers [EERE]

    Biological Hydrogen Production Workshop Summary Report 2013 Biological Hydrogen Production Workshop Summary Report November 2013 summary report for the 2013 Biological Hydrogen ...

  3. Synthetic Biology and Bioenergy: Helping the Good Guy and Stopping the Bad (2014 DOE JGI Genomics of Energy & Environment Meeting)

    SciTech Connect (OSTI)

    Mercedes-Roca, Maria [Zamorano Panamerican Agriculture School

    2014-03-19

    Maria Mercedes-Roca of the Zamorano Panamerican Agriculture School speaks at the 9th Annual Genomics of Energy & Environment Meeting on March 20, 2014 in Walnut Creek, Calif.

  4. Apodized RFI filtering of synthetic aperture radar images

    SciTech Connect (OSTI)

    Doerry, Armin Walter

    2014-02-01

    Fine resolution Synthetic Aperture Radar (SAR) systems necessarily require wide bandwidths that often overlap spectrum utilized by other wireless services. These other emitters pose a source of Radio Frequency Interference (RFI) to the SAR echo signals that degrades SAR image quality. Filtering, or excising, the offending spectral contaminants will mitigate the interference, but at a cost of often degrading the SAR image in other ways, notably by raising offensive sidelobe levels. This report proposes borrowing an idea from nonlinear sidelobe apodization techniques to suppress interference without the attendant increase in sidelobe levels. The simple post-processing technique is termed Apodized RFI Filtering (ARF).

  5. Phase correction system for automatic focusing of synthetic aperture radar

    DOE Patents [OSTI]

    Eichel, Paul H. (Albuquerque, NM); Ghiglia, Dennis C. (Placitas, NM); Jakowatz, Jr., Charles V. (Albuquerque, NM)

    1990-01-01

    A phase gradient autofocus system for use in synthetic aperture imaging accurately compensates for arbitrary phase errors in each imaged frame by locating highlighted areas and determining the phase disturbance or image spread associated with each of these highlight areas. An estimate of the image spread for each highlighted area in a line in the case of one dimensional processing or in a sector, in the case of two-dimensional processing, is determined. The phase error is determined using phase gradient processing. The phase error is then removed from the uncorrected image and the process is iteratively performed to substantially eliminate phase errors which can degrade the image.

  6. Synthetic aperture radar images with composite azimuth resolution

    DOE Patents [OSTI]

    Bielek, Timothy P; Bickel, Douglas L

    2015-03-31

    A synthetic aperture radar (SAR) image is produced by using all phase histories of a set of phase histories to produce a first pixel array having a first azimuth resolution, and using less than all phase histories of the set to produce a second pixel array having a second azimuth resolution that is coarser than the first azimuth resolution. The first and second pixel arrays are combined to produce a third pixel array defining a desired SAR image that shows distinct shadows of moving objects while preserving detail in stationary background clutter.

  7. Organization | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    About Us Organization Organization Organization Download Printable PDF PDF icon Organization Chart - Dated: 07122015 Key Resources PMCDP EVMS PARS IIe FPD Resource Center PM ...

  8. Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.; Yang, Xiaohan; Cheng, Zong-Ming; Chen, Jin-Gui; Tuskan, Gerald A.

    2016-02-17

    Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs ormore » multiple transformation events that is often restricted by a lack of available transformation markers. Synthetic RNAi fragments were assembled in yeast carrying homologous sequences to six or seven non-family genes and introduced into pAGRIKOLA. Transformation of Arabidopsis thaliana and subsequent expression analysis of targeted genes proved efficient knockdown of all target genes. In conclusion, we present a simple and cost-effective method to create constructs to simultaneously knockdown multiple non-family genes or genes that do not share sequence homology. The presented method can be applied in plant and animal synthetic biology as well as traditional plant and animal genetic engineering.« less

  9. Preparation of hydrophobic organic aeorgels

    DOE Patents [OSTI]

    Baumann, Theodore F.; Satcher, Jr., Joe H.; Gash, Alexander E.

    2007-11-06

    Synthetic methods for the preparation of hydrophobic organics aerogels. One method involves the sol-gel polymerization of 1,3-dimethoxybenzene or 1,3,5-trimethoxybenzene with formaldehyde in non-aqueous solvents. Using a procedure analogous to the preparation of resorcinol-formaldehyde (RF) aerogels, this approach generates wet gels that can be dried using either supercritical solvent extraction to generate the new organic aerogels or air dried to produce an xerogel. Other methods involve the sol-gel polymerization of 1,3,5 trihydroxy benzene (phloroglucinol) or 1,3 dihydroxy benzene (resorcinol) and various aldehydes in non-aqueous solvents. These methods use a procedure analogous to the one-step base and two-step base/acid catalyzed polycondensation of phloroglucinol and formaldehyde, but the base catalyst used is triethylamine. These methods can be applied to a variety of other sol-gel precursors and solvent systems. These hydrophobic organics aerogels have numerous application potentials in the field of material absorbers and water-proof insulation.

  10. Preparation of hydrophobic organic aeorgels

    DOE Patents [OSTI]

    Baumann, Theodore F.; Satcher, Jr., Joe H.; Gash, Alexander E.

    2004-10-19

    Synthetic methods for the preparation of hydrophobic organics aerogels. One method involves the sol-gel polymerization of 1,3-dimethoxybenzene or 1,3,5-trimethoxybenzene with formaldehyde in non-aqueous solvents. Using a procedure analogous to the preparation of resorcinol-formaldehyde (RF) aerogels, this approach generates wet gels that can be dried using either supercritical solvent extraction to generate the new organic aerogels or air dried to produce an xerogel. Other methods involve the sol-gel polymerization of 1,3,5 trihydroxy benzene (phloroglucinol) or 1,3 dihydroxy benzene (resorcinol) and various aldehydes in non-aqueous solvents. These methods use a procedure analogous to the one-step base and two-step base/acid catalyzed polycondensation of phloroglucinol and formaldehyde, but the base catalyst used is triethylamine. These methods can be applied to a variety of other sol-gel precursors and solvent systems. These hydrophobic organics aerogels have numerous application potentials in the field of material absorbers and water-proof insulation.

  11. Biological Safety | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Select Agents Inspection Report: IG-0681, Concerns Regarding a Non-Viable (Dead) "Anthrax Sport" Research Project at the Oak Ridge National Laboratory Inspection ...

  12. Biological Applications of Synchrotron Radiation:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Biological Applications of Synchrotron Radiation: An Evaluation of the State of the Field ... Maxwell's equations show that electromagnetic radiation is generated when charged ...

  13. Biological & Environmental Research Abstracts Database

    Office of Scientific and Technical Information (OSTI)

    Welcome to the Biological and Environmental Research Abstracts Database The U.S. ... This database contains abstracts of research projects supported by the program. Work was ...

  14. 6th Annual Systems Biology Symposium: Systems Biology and the Environment

    SciTech Connect (OSTI)

    Galitski, Timothy, P.

    2007-04-01

    Systems biology recognizes the complex multi-scale organization of biological systems, from molecules to ecosystems. The International Symposium on Systems Biology is an annual two-day event gathering the most influential researchers transforming biology into an integrative discipline investigating complex systems. In recognition of the fundamental similarity between the scientific problems addressed in environmental science and systems biology studies at the molecular, cellular, and organismal levels, the 2007 Symposium featured global leaders in “Systems Biology and the Environment.” The objective of the 2007 “Systems Biology and the Environment” International Symposium was to stimulate interdisciplinary thinking and research that spans systems biology and environmental science. This Symposium was well aligned with the DOE’s Genomics:GTL program efforts to achieve scientific objectives for each of the three DOE missions: • Develop biofuels as a major secure energy source for this century, • Develop biological solutions for intractable environmental problems, and • Understand biosystems’ climate impacts and assess sequestration strategies Our scientific program highlighted world-class research exemplifying these priorities. The Symposium featured 45 minute lectures from 12 researchers including: Penny/Sallie Chisholm of MIT gave the keynote address “Tiny Cells, Global Impact: What Prochlorococcus Can Teach Us About Systems Biology”, plus Jim Fredrickson of PNNL, Nitin Baliga of ISB, Steve Briggs of UCSD, David Cox of Perlegen Sciences, Antoine Danchin of Institut Pasteur, John Delaney of the U of Washington, John Groopman of Johns Hopkins, Ben Kerr of the U of Washington, Steve Koonin of BP, Elliott Meyerowitz of Caltech, and Ed Rubin of LBNL. The 2007 Symposium promoted DOE’s three mission areas among scientists from multiple disciplines representing academia, non-profit research institutions, and the private sector. As in all previous

  15. AN INTEGRATED BIOLOGICAL CONTROL SYSTEM AT HANFORD

    SciTech Connect (OSTI)

    JOHNSON AR; CAUDILL JG; GIDDINGS RF; RODRIGUEZ JM; ROOS RC; WILDE JW

    2010-02-11

    In 1999 an integrated biological control system was instituted at the U.S. Department of Energy's Hanford Site. Successes and changes to the program needed to be communicated to a large and diverse mix of organizations and individuals. Efforts at communication are directed toward the following: Hanford Contractors (Liquid or Tank Waste, Solid Waste, Environmental Restoration, Science and Technology, Site Infrastructure), General Hanford Employees, and Hanford Advisory Board (Native American Tribes, Environmental Groups, Local Citizens, Washington State and Oregon State regulatory agencies). Communication was done through direct interface meetings, individual communication, where appropriate, and broadly sharing program reports. The objectives of the communication efforts was to have the program well coordinated with Hanford contractors, and to have the program understood well enough that all stakeholders would have confidence in the work performed by the program to reduce or elimated spread of radioactive contamination by biotic vectors. Communication of successes and changes to an integrated biological control system instituted in 1999 at the Department of Energy's Hanford Site have required regular interfaces with not only a diverse group of Hanford contractors (i.e., those responsible for liquid or tank waste, solid wastes, environmental restoration, science and technology, and site infrastructure), and general Hanford employees, but also with a consortium of designated stake holders organized as the Hanford Advisory Board (i.e., Native American tribes, various environmental groups, local citizens, Washington state and Oregon regulatory agencies, etc.). Direct interface meetings, individual communication where appropriate, and transparency of the biological control program were the methods and outcome of this effort.

  16. Modular synthetic inverters from zinc finger proteins and small RNAs

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hsia, Justin; Holtz, William J.; Maharbiz, Michel M.; Arcak, Murat; Keasling, Jay D.; Rao, Christopher V.

    2016-02-17

    Synthetic zinc finger proteins (ZFPs) can be created to target promoter DNA sequences, repressing transcription. The binding of small RNA (sRNA) to ZFP mRNA creates an ultrasensitive response to generate higher effective Hill coefficients. Here we combined three “off the shelf” ZFPs and three sRNAs to create new modular inverters in E. coli and quantify their behavior using induction fold. We found a general ordering of the effects of the ZFPs and sRNAs on induction fold that mostly held true when combining these parts. We then attempted to construct a ring oscillator using our new inverters. In conclusion, our chosenmore » parts performed insufficiently to create oscillations, but we include future directions for improvement upon our work presented here.« less

  17. Interpretation of Synthetic Aperture Radar measurements of ocean currents

    SciTech Connect (OSTI)

    Rufenach, C.L.; Shuchman, R.A.; Lyzenga, D.R.

    1983-02-28

    Synthetic Aperture Radar (SAR) experiments hae been performed over the last few years to measure ocean currents inferred from shifts in the Doppler spectral peak. Interpretations of aircraft SAR measurements, when compared with limited surface values, tend to underestimate the currents by about 25%. A theory is developed that modifies the classical Doppler expression showing that the radar measurements are dependent on the radar processor (system) bandwidth and the received signal bandwidth. Measured bandwidths give a correction that increases the inferred current values by about 25%, bringing the measurements into good agreement. This new correction lends credence to the theory and increases the potential for application of SAR systems to future ocean current measurements. SAR measurements should include the determination of processor and signal bandwidths such that this correction can be applied.

  18. Moving receive beam method and apparatus for synthetic aperture radar

    DOE Patents [OSTI]

    Kare, Jordin T.

    2001-01-01

    A method and apparatus for improving the performance of Synthetic Aperture Radar (SAR) systems by reducing the effect of "edge losses" associated with nonuniform receiver antenna gain. By moving the receiver antenna pattern in synchrony with the apparent motion of the transmitted pulse along the ground, the maximum available receiver antenna gain can be used at all times. Also, the receiver antenna gain for range-ambiguous return signals may be reduced, in some cases, by a large factor. The beam motion can be implemented by real-time adjustment of phase shifters in an electronically-steered phased-array antenna or by electronic switching of feed horns in a reflector antenna system.

  19. Time-frequency analysis of synthetic aperture radar signals

    SciTech Connect (OSTI)

    Johnston, B.

    1996-08-01

    Synthetic aperture radar (SAR) has become an important tool for remote sensing of the environment. SAR is a set of digital signal processing algorithms that are used to focus the signal returned to the radar because radar systems in themselves cannot produce the high resolution images required in remote sensing applications. To reconstruct an image, several parameters must be estimated and the quality of output image depends on the degree of accuracy of these parameters. In this thesis, we derive the fundamental SAR algorithms and concentrate on the estimation of one of its critical parameters. We show that the common technique for estimating this particular parameter can sometimes lead to erroneous results and reduced quality images. We also employ time-frequency analysis techniques to examine variations in the radar signals caused by platform motion and show how these results can be used to improve output image quality.

  20. Method for biological purification

    DOE Patents [OSTI]

    Lucido, John A.; Keenan, Daniel; Premuzic, Eugene T.; Lin, Mow S.; Shelenkova, Ludmila

    2001-03-27

    An apparatus is disclosed for containing a microorganism culture in an active exponential growth and delivering a supply of microorganisms to an environment containing wastes for bio-augmenting the biodegradation of the wastes. The apparatus comprises a bioreactor and an operably connected controller. The bioreactor has a bioreactor chamber for containing a supply of microorganisms, a second chamber for containing a supply of water and inorganic nutrients, and a third chamber for containing a supply of organic nutrients. The bioreactor is operably connected to the controller in which a first pump is operably connected in fluid communication between the bioreactor chamber and the second chamber and third chamber, and a second pump is operably connected in fluid communication between the bioreactor chamber and the environment containing wastes to be biodegraded. The controller further includes a timer and regulator operably connected to the first and second pumps to effectively maintain the microorganisms in exponential growth in the bioreactor chamber and to deliver microorganisms to an environment to be treated. Also, disclosed is a method for bio-augmenting the biodegradation of wastes.

  1. Organization Chart - Home

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LSD Logo About Us People & Organization Research News & Events Safety Internal Resources Organization Chart Departments Scientific Staff Directory Committees Organization Chart...

  2. Kinetically tuned dimensional augmentation as a versatile synthetic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    5, 5723 (2014) DOI: 10.1038ncomms6723 Abstract: Metal-organic frameworks with high stability have been pursued for many years due to the sustainability requirement for...

  3. Synthetic fuel concept to steal CO2 from air

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    concept, called Green Freedom(tm), for large-scale production of carbon-neutral, sulfur-free fuels and organic chemicals from air and water. February 12, 2008 Los Alamos National...

  4. ANAEROBIC BIOLOGICAL TREATMENT OF PRODUCED WATER

    SciTech Connect (OSTI)

    John R. Gallagher

    2001-07-31

    During the production of oil and gas, large amounts of water are brought to the surface and must be disposed of in an environmentally sensitive manner. This is an especially difficult problem in offshore production facilities where space is a major constraint. The chief regulatory criterion for produced water is oil and grease. Most facilities have little trouble meeting this criterion using conventional oil-water separation technologies. However, some operations have significant amounts of naphthenic acids in the water that behave as oil and grease but are not well removed by conventional technologies. Aerobic biological treatment of naphthenic acids in simulated-produced water has been demonstrated by others; however, the system was easily overloaded by the large amounts of low-molecular-weight organic acids often found in produced waters. The objective of this research was to determine the ability of an anaerobic biological system to treat these organic acids in a simulated produced water and to examine the potential for biodegradation of the naphthenic acids in the anaerobic environment. A small fixed-film anaerobic biological reactor was constructed and adapted to treat a simulated produced water. The bioreactor was tubular, with a low-density porous glass packing material. The inocula to the reactor was sediment from a produced-water holding pond from a municipal anaerobic digester and two salt-loving methanogenic bacteria. During start-up, the feed to the reactor contained glucose as well as typical produced-water components. When glucose was used, rapid gas production was observed. However, when glucose was eliminated and the major organic component was acetate, little gas was generated. Methane production from acetate may have been inhibited by the high salt concentrations, by sulfide, or because of the lack, despite seeding, of microbes capable of converting acetate to methane. Toluene, a minor component of the produced water (0.1 g/L) was removed in the

  5. Photoreactive synthetic regulator of protein function and methods of use thereof

    DOE Patents [OSTI]

    Trauner, Dirk; Isacoff, Ehud Y; Kramer, Richard H; Banghart, Matthew R; Fortin, Doris L; Mourot, Alexandre

    2015-03-31

    The present disclosure provides a photoreactive synthetic regulator of protein function. The present disclosure further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present disclosure further provides methods of modulating protein function, involving use of light.

  6. Uranium Removal from Contaminated Groundwater by Synthetic Resins

    SciTech Connect (OSTI)

    Phillips, Debra H.; Gu, Baohua; Watson, David B; Parmele, C. S.

    2008-01-01

    Synthetic resins are shown to be effective in removing uranium from contaminated groundwater. Batch and field column tests showed that strong-base anion-exchange resins were more effective in removing uranium from both near-neutral-pH (6.5)- and high-pH (8)-low-nitrate-containing ground waters, than metal-chelating resins, which removed more uranium from acidic-pH (5)-high-nitrate-containing groundwater from the Oak Ridge Reservation (ORR) Y-12 S-3 Ponds area in Tennessee, USA. Dowex 1-X8 and Purolite A-520E anion-exchange resins removed more uranium from high-pH (8)-low-nitrate-containing synthetic groundwater in batch tests than metal-chelating resins. The Dowex{trademark} 21K anion-exchange resin achieved a cumulative loading capacity of 49.8 mg g{sup -1} before breakthrough in a field column test using near-neutral-pH (6.5)-low-nitrate-containing groundwater. However, in an acidic-pH (5)-high-nitrate-containing groundwater, metal-chelating resins Diphonix and Chelex-100 removed more uranium than anion-exchange resins. In 15 mL of acidic-pH (5)-high-nitrate-containing groundwater spiked with 20 mg L{sup -1} uranium, the uranium concentrations ranged from 0.95 mg L{sup -1} at 1-h equilibrium to 0.08 mg L{sup -1} at 24-h equilibrium for Diphonix and 0.17 mg L{sup -1} at 1-h equilibrium to 0.03 mg L{sup -1} at 24-h equilibrium for Chelex-100. Chelex-100 removed more uranium in the first 10 min in the 100 mL of acidic-(pH 5)-high-nitrate-containing groundwater (5 mg L{sup -1} uranium); however, after 10 min, Diphonix equaled or out-performed Chelex-100. This study presents an improved understanding of the selectivity and sorption kinetics of a range of ion-exchange resins that remove uranium from both low- and high-nitrate-containing groundwaters with varying pHs.

  7. Biological Science | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Biological Science Biological Science The protozoan Plasmodium falciparum gliding through a cell in the gut of a mosquito, its primary host. Although five different species of Plasmodium can cause malaria, Plasmodium falciparum causes the most severe disease. | Photo courtesy of Wikipedia Commons. <a href="http://energy.gov/articles/malaria-researchers-find-weakness-global-killer">Read more</a> The protozoan Plasmodium falciparum gliding through a cell in the gut of a

  8. Michael Levitt and Computational Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Michael Levitt and Computational Biology Resources with Additional Information * Publications Michael Levitt Courtesy of Linda A. Cicero / Stanford News Service Michael Levitt, PhD, professor of structural biology at the Stanford University School of Medicine, has won the 2013 Nobel Prize in Chemistry. ... Levitt ... shares the ... prize with Martin Karplus ... and Arieh Warshel ... "for the development of multiscale models for complex chemical systems." Levitt's work focuses on

  9. A single crystalline porphyrinic titanium metalorganic framework

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yuan, Shuai; Liu, Tian -Fu; Feng, Dawei; Tian, Jian; Wang, Kecheng; Qin, Junsheng; Zhang, Qiang; Chen, Ying -Pin; Bosch, Mathieu; Zou, Lanfang; et al

    2015-04-28

    We successfully assembled the photocatalytic titanium-oxo cluster and photosensitizing porphyrinic linker into a metalorganic framework (MOF), namely PCN-22. A preformed titanium-oxo carboxylate cluster is adopted as the starting material to judiciously control the MOF growth process to afford single crystals. This synthetic method is useful to obtain highly crystalline titanium MOFs, which has been a daunting challenge in this field. Moreover, PCN-22 demonstrated permanent porosity and photocatalytic activities toward alcohol oxidation.

  10. A single crystalline porphyrinic titanium metal–organic framework

    SciTech Connect (OSTI)

    Yuan, Shuai; Liu, Tian -Fu; Feng, Dawei; Tian, Jian; Wang, Kecheng; Qin, Junsheng; Zhang, Qiang; Chen, Ying -Pin; Bosch, Mathieu; Zou, Lanfang; Teat, Simon J.; Dalgarno, Scott J.; Zhou, Hong -Cai

    2015-04-28

    We successfully assembled the photocatalytic titanium-oxo cluster and photosensitizing porphyrinic linker into a metal–organic framework (MOF), namely PCN-22. A preformed titanium-oxo carboxylate cluster is adopted as the starting material to judiciously control the MOF growth process to afford single crystals. This synthetic method is useful to obtain highly crystalline titanium MOFs, which has been a daunting challenge in this field. Moreover, PCN-22 demonstrated permanent porosity and photocatalytic activities toward alcohol oxidation.

  11. Pore surface engineering in a zirconium metal–organic framework via thiol-ene reaction

    SciTech Connect (OSTI)

    Gui, Bo; Hu, Guiping; Zhou, Tailin; Wang, Cheng

    2015-03-15

    A porous olefin-functionalized Zr(IV)-based metal–organic framework, denoted as UiO-68-allyl, has been constructed. Our results clearly demonstrated that the surface of UiO-68-allyl could be decorated with organic molecule (ethanethiol) via thiol-ene reaction. More importantly, the crystallinity of the framework were maintained during the post-synthetic modification process. However, the microporosity of the framework is retained but the surface area decreased, due to the grafting of ethylthio groups into the pores. From our studies, we can conclude that the strategy of post-synthetic modification of UiO-68-allyl via thiol-ene reaction may be general. Furthermore, we may anchor other desired functional group onto the pore walls in Zr-MOFs via thiol-ene reaction, enabling more potential applications. - graphical abstract: In this manuscript, we reported the post-synthetic modification of an olefin-functionalized Zr(IV)-based metal–organic framework via thiol-ene reaction. - Highlights: • A porous olefin-functionalized Zr(IV)-based metal–organic framework has been constructed. • The surface of olefin-functionalized Zr-MOF could be decorated with organic molecules via thiol-ene reaction. • The crystallinity and permanent porosity of the framework were maintained during the post-synthetic modification process.

  12. Lynx: A High-Resolution Synthetic Aperture Radar

    SciTech Connect (OSTI)

    Doerry, A.W.; Hensley, W.H.; Pace, F.; Stence, J.; Tsunoda, S.I.; Walker, B.C.; Woodring, M.

    1999-03-08

    Lynx is a high resolution, synthetic aperture radar (SAR) that has been designed and built by Sandia National Laboratories in collaboration with General Atomics (GA). Although Lynx may be operated on a wide variety of manned and unmanned platforms, it is primarily intended to be fielded on unmanned aerial vehicles. In particular, it may be operated on the Predator, I-GNAT, or Prowler II platforms manufactured by GA Aeronautical Systems, Inc. The Lynx production weight is less than 120 lb. and has a slant range of 30 km (in 4 mm/hr rain). It has operator selectable resolution and is capable of 0.1 m resolution in spotlight mode and 0.3 m resolution in stripmap mode. In ground moving target indicator mode, the minimum detectable velocity is 6 knots with a minimum target cross-section of 10 dBsm. In coherent change detection mode, Lynx makes registered, complex image comparisons either of 0.1 m resolution (minimum) spotlight images or of 0.3 m resolution (minimum) strip images. The Lynx user interface features a view manager that allows it to pan and zoom like a video camera. Lynx was developed under corporate finding from GA and will be manufactured by GA for both military and commercial applications. The Lynx system architecture will be presented and some of its unique features will be described. Imagery at the finest resolutions in both spotlight and strip modes have been obtained and will also be presented.

  13. Biological and Environmental Research Network Requirements

    SciTech Connect (OSTI)

    Balaji, V.; Boden, Tom; Cowley, Dave; Dart, Eli; Dattoria, Vince; Desai, Narayan; Egan, Rob; Foster, Ian; Goldstone, Robin; Gregurick, Susan; Houghton, John; Izaurralde, Cesar; Johnston, Bill; Joseph, Renu; Kleese-van Dam, Kerstin; Lipton, Mary; Monga, Inder; Pritchard, Matt; Rotman, Lauren; Strand, Gary; Stuart, Cory; Tatusova, Tatiana; Tierney, Brian; Thomas, Brian; Williams, Dean N.; Zurawski, Jason

    2013-09-01

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the U.S. Department of Energy (DOE) Office of Science (SC), the single largest supporter of basic research in the physical sciences in the United States. In support of SC programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet be a highly successful enabler of scientific discovery for over 25 years. In November 2012, ESnet and the Office of Biological and Environmental Research (BER) of the DOE SC organized a review to characterize the networking requirements of the programs funded by the BER program office. Several key findings resulted from the review. Among them: 1) The scale of data sets available to science collaborations continues to increase exponentially. This has broad impact, both on the network and on the computational and storage systems connected to the network. 2) Many science collaborations require assistance to cope with the systems and network engineering challenges inherent in managing the rapid growth in data scale. 3) Several science domains operate distributed facilities that rely on high-performance networking for success. Key examples illustrated in this report include the Earth System Grid Federation (ESGF) and the Systems Biology Knowledgebase (KBase). This report expands on these points, and addresses others as well. The report contains a findings section as well as the text of the case studies discussed at the review.

  14. Inorganic Metal Oxide/Organic Polymer Nanocomposites And Method Thereof

    DOE Patents [OSTI]

    Gash, Alexander E.; Satcher, Joe H.; Simpson, Randy

    2004-11-16

    A synthetic method for preparation of hybrid inorganic/organic energetic nanocomposites is disclosed herein. The method employs the use of stable metal in organic salts and organic solvents as well as an organic polymer with good solubility in the solvent system to produce novel nanocomposite energetic materials. In addition, fuel metal powders (particularly those that are oxophilic) can be incorporated into composition. This material has been characterized by thermal methods, energy-filtered transmission electron microscopy (EFTEM), N.sub.2 adsoprtion/desorption methods, and Fourier-Transform (FT-IR) spectroscopy. According to these characterization methods the organic polymer phase fills the nanopores of the material, providing superb mixing of the component phases in the energetic nanocomposite.

  15. Autofermentative Biological Hydrogen Production by Cyanobacteria...

    Broader source: Energy.gov (indexed) [DOE]

    More Documents & Publications 2013 Biological Hydrogen Production Workshop Summary Report Renewable Hydrogen Production from Biological Systems Anthropogenic CO2 as a Feedstock for ...

  16. Autofermentative Biological Hydrogen Production by Cyanobacteria

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    BioSolarH 2 Autofermentative biological hydrogen production by cyanobacteria G.C. Dismukes Rutgers University Waksman Institute and Department of Chemistry & Chemical Biology ...

  17. Innovative Breakthrough Demonstrated for Biological Ethanol Production...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovative Breakthrough Demonstrated for Biological Ethanol Production Innovative Breakthrough Demonstrated for Biological Ethanol Production June 30, 2015 - 11:43am Addthis ...

  18. Management Principles for Nonproliferation Organizations

    SciTech Connect (OSTI)

    Frazar, Sarah L.; Hund, Gretchen

    2012-03-06

    This paper identifies business models and six management principles that can be applied by a nonproliferation organization to maximize the value and effectiveness of its products. The organizations responsible for reducing the nuclear proliferation threat have experienced a substantial growth in responsibility and visibility since the September 11 attacks. Since then, the international community has witnessed revelations of clandestine nuclear facilities, nuclear black markets, periodic nuclear tests, and a resurgence of interest by countries worldwide in developing nuclear capabilities. The security environment will likely continue to evolve in unexpected ways since most of the proliferation threats with which the world will be forced to contend remain unforeseen. To better prepare for and respond to this evolving security environment, many nonproliferation organizations are interested in finding new or better ways to increase the effectiveness and efficiency of their operations. Of course, all organizations, whether they are market driven or non-profit, must operate effectively and efficiently if they are to succeed. Indeed, as this study demonstrates, many of the management principles that this study recommends can help all organizations succeed. However, this study pays particular attention to nonproliferation organizations because of the mission they are responsible for fulfilling. Nonproliferation organizations, including nonproliferation programs that operate within a larger national security organization, are responsible for reducing the threat of nuclear, chemical and biological weapons. These organizations have an enduring mandate from the public and the international community not to fail in the completion of their mission for failure could have detrimental impacts on international security, public health and the environment. Moreover, the public expects nonproliferation organizations and programs to fulfill their mission, even when resources are limited

  19. Biomimicry in metal-organic materials

    SciTech Connect (OSTI)

    Zhang, MW; Gu, ZY; Bosch, M; Perry, Z; Zhou, HC

    2015-06-15

    Nature has evolved a great number of biological molecules which serve as excellent constructional or functional units for metal-organic materials (MOMs). Even though the study of biomimetic MOMs is still at its embryonic stage, considerable progress has been made in the past few years. In this critical review, we will highlight the recent advances in the design, development and application of biomimetic MOMs, and illustrate how the incorporation of biological components into MOMs could further enrich their structural and functional diversity. More importantly, this review will provide a systematic overview of different methods for rational design of MOMs with biomimetic features. Published by Elsevier B.V.

  20. Thin film composition with biological substance and method of making

    DOE Patents [OSTI]

    Campbell, Allison A.; Song, Lin

    1999-01-01

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphus structures, organic crystalline structures, and organic amorphus structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobal, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflamatory, steriod, nonsteriod anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor the compositions listed above.

  1. Thin film composition with biological substance and method of making

    SciTech Connect (OSTI)

    Campbell, A.A.; Song, L.

    1999-09-28

    The invention provides a thin-film composition comprising an underlying substrate of a first material including a plurality of attachment sites; a plurality of functional groups chemically attached to the attachment sites of the underlying substrate; and a thin film of a second material deposited onto the attachment sites of the underlying substrate, and a biologically active substance deposited with the thin-film. Preferably the functional groups are attached to a self assembling monolayer attached to the underlying substrate. Preferred functional groups attached to the underlying substrate are chosen from the group consisting of carboxylates, sulfonates, phosphates, optionally substituted, linear or cyclo, alkyl, alkene, alkyne, aryl, alkylaryl, amine, hydroxyl, thiol, silyl, phosphoryl, cyano, metallocenyl, carbonyl, and polyphosphate. Preferred materials for the underlying substrate are selected from the group consisting of a metal, a metal alloy, a plastic, a polymer, a proteic film, a membrane, a glass or a ceramic. The second material is selected from the group consisting of inorganic crystalline structures, inorganic amorphous structures, organic crystalline structures, and organic amorphous structures. Preferred second materials are phosphates, especially calcium phosphates and most particularly calcium apatite. The biologically active molecule is a protein, peptide, DNA segment, RNA segment, nucleotide, polynucleotide, nucleoside, antibiotic, antimicrobial, radioisotope, chelated radioisotope, chelated metal, metal salt, anti-inflammatory, steroid, nonsteroid anti-inflammatory, analgesic, antihistamine, receptor binding agent, or chemotherapeutic agent, or other biologically active material. Preferably the biologically active molecule is an osteogenic factor consisting of the compositions listed above.

  2. Ordered organic-organic multilayer growth

    DOE Patents [OSTI]

    Forrest, Stephen R; Lunt, Richard R

    2015-01-13

    An ordered multilayer crystalline organic thin film structure is formed by depositing at least two layers of thin film crystalline organic materials successively wherein the at least two thin film layers are selected to have their surface energies within .+-.50% of each other, and preferably within .+-.15% of each other, whereby every thin film layer within the multilayer crystalline organic thin film structure exhibit a quasi-epitaxial relationship with the adjacent crystalline organic thin film.

  3. Ordered organic-organic multilayer growth

    DOE Patents [OSTI]

    Forrest, Stephen R.; Lunt, Richard R.

    2016-04-05

    An ordered multilayer crystalline organic thin film structure is formed by depositing at least two layers of thin film crystalline organic materials successively wherein the at least two thin film layers are selected to have their surface energies within .+-.50% of each other, and preferably within .+-.15% of each other, whereby every thin film layer within the multilayer crystalline organic thin film structure exhibit a quasi-epitaxial relationship with the adjacent crystalline organic thin film.

  4. Systems biology approach to bioremediation

    SciTech Connect (OSTI)

    Chakraborty, Romy; Wu, Cindy H.; Hazen, Terry C.

    2012-06-01

    Bioremediation has historically been approached as a ‘black box’ in terms of our fundamental understanding. Thus it succeeds and fails, seldom without a complete understanding of why. Systems biology is an integrated research approach to study complex biological systems, by investigating interactions and networks at the molecular, cellular, community, and ecosystem level. The knowledge of these interactions within individual components is fundamental to understanding the dynamics of the ecosystem under investigation. Finally, understanding and modeling functional microbial community structure and stress responses in environments at all levels have tremendous implications for our fundamental understanding of hydrobiogeochemical processes and the potential for making bioremediation breakthroughs and illuminating the ‘black box’.

  5. Synthetic Coal Slag Infiltration into Varying Refractory Materials

    SciTech Connect (OSTI)

    Kaneko, Tetsuya K; Thomas, Hugh; Bennett, James P; Sridhar, Seetharaman

    2012-10-01

    The infiltrations of synthetic coal slag into 99%Al{sub 2}O{sub 3}, 85%Al{sub 2}O{sub 3}–15%SiO{sub 2}, and 90%Cr{sub 2}O{sub 3}–10%Al{sub 2}O{sub 3} refractories with a temperature gradient induced along the penetration direction were compared to one another. The infiltrating slag was synthesized with a composition that is representative of an average of the ash contents from U S coal feedstock. Experiments were conducted with a hot-face temperature of 1450°C in a CO/CO{sub 2} atmosphere. Minimal penetration was observed in the 90%Cr{sub 2}O{sub 3}–10%Al{sub 2}O{sub 3} material because interactions between the refractory and the slag produced a protective layer of FeCr{sub 2}O{sub 4}, which impeded slag flow into the bulk of the refractory. After 5 h, the 99%Al{sub 2}O{sub 3} sample exhibited an average penetration of 12.7 mm whereas the 85%Al{sub 2}O{sub 3}–15%SiO{sub 2} sample showed 3.8 mm. Slag infiltrated into the 99%Al{sub 2}O{sub 3} and 85%Al{sub 2}O{sub 3}–15%SiO{sub 2} refractory systems by dissolving the respective refractories' matrix materials, which consist of fine Al{sub 2}O{sub 3} particles and an amorphous alumino-silicate phase. Due to enrichment in SiO{sub 2}, a network-former, infiltration into the 85%Al{sub 2}O{sub 3}–15%SiO{sub 2} system yielded a higher viscosity slag and hence, a shallower penetration depth. The results suggest that slag infiltration can be limited by interactions with the refractory through the formation of either a solid layer that physically impedes fluid flow or a more viscous slag that retards infiltration.

  6. Perpendicular magnetic tunnel junctions with double barrier and single or synthetic antiferromagnetic storage layer

    SciTech Connect (OSTI)

    Cuchet, La; Rodmacq, Bernard; Auffret, Stphane; Sousa, Ricardo C.; Prejbeanu, Ioan L.; Dieny, Bernard

    2015-06-21

    The magnetic properties of double tunnel junctions with perpendicular anisotropy were investigated. Two synthetic antiferromagnetic references are used, while the middle storage magnetic layer can be either a single ferromagnetic or a synthetic antiferromagnetic FeCoB-based layer, with a critical thickness as large as 3.0?nm. Among the different achievable magnetic configurations in zero field, those with either antiparallel references, and single ferromagnetic storage layer, or parallel references, and synthetic antiferromagnetic storage layer, are of particular interest since they allow increasing the efficiency of spin transfer torque writing and the thermal stability of the stored information as compared to single tunnel junctions. The latter configuration can be preferred when stray fields would favour a parallel orientation of the reference layers. In this case, the synthetic antiferromagnetic storage layer is also less sensitive to residual stray fields.

  7. Lighting system with thermal management system having point contact synthetic jets

    DOE Patents [OSTI]

    Arik, Mehmet; Weaver, Stanton Earl; Kuenzler, Glenn Howard; Wolfe, Jr, Charles Franklin; Sharma, Rajdeep

    2016-08-30

    Lighting systems having unique configurations are provided. For instance, the lighting system may include a light source, a thermal management system and driver electronics, each contained within a housing structure. The light source is configured to provide illumination visible through an opening in the housing structure. The thermal management system includes a plurality of synthetic jets. The synthetic jets are arranged within the lighting system such that they are secured at contact points.

  8. Lighting system with thermal management system having point contact synthetic jets

    SciTech Connect (OSTI)

    Arik, Mehmet; Weaver, Stanton Earl; Kuenzler, Glenn Howard; Wolfe, Jr., Charles Franklin; Sharma, Rajdeep

    2013-12-10

    Lighting system having unique configurations are provided. For instance, the lighting system may include a light source, a thermal management system and driver electronics, each contained within a housing structure. The light source is configured to provide illumination visible through an opening in the housing structure. The thermal management system includes a plurality of synthetic jets. The synthetic jets are arranged within the lighting system such that they are secured at contact points.

  9. Lighting system with thermal management system having point contact synthetic jets

    DOE Patents [OSTI]

    Arik, Mehmet; Weaver, Stanton Earl; Kuenzler, Glenn Howard; Wolfe, Jr., Charles Franklin; Sharma, Rajdeep

    2016-08-23

    Lighting systems having unique configurations are provided. For instance, the lighting system may include a light source, a thermal management system and driver electronics, each contained within a housing structure. The light source is configured to provide illumination visible through an opening in the housing structure. The thermal management system includes a plurality of synthetic jets. The synthetic jets are arranged within the lighting system such that they are secured at contact points.

  10. Process for gasification using a synthetic CO.sub.2 acceptor

    DOE Patents [OSTI]

    Lancet, Michael S.; Curran, George P.

    1980-01-01

    A gasification process is disclosed using a synthetic CO.sub.2 acceptor consisting essentially of at least one compound selected from the group consisting of calcium oxide and calcium carbonate supported in a refractory carrier matrix, the carrier having the general formula Ca.sub.5 (SiO.sub.4).sub.2 CO.sub.3. A method for producing the synthetic CO.sub.2 acceptor is also disclosed.