Sample records for nucleic acids res

  1. Cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor L. (Madison, WI); Brow, Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2007-12-11T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  2. Nucleic acid detection compositions

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor I. (Madison, WI); Brow, Mary Ann (Madison, WI); Dahlberg, James L. (Madison, WI)

    2008-08-05T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  3. Cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Waunakee, WI); Lyamichev, Victor I. (Madison, WI); Brow; Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2010-11-09T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  4. Nucleic acid detection assays

    DOE Patents [OSTI]

    Prudent, James R.; Hall, Jeff G.; Lyamichev, Victor I.; Brow, Mary Ann; Dahlberg, James E.

    2005-04-05T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  5. Nucleic acid detection kits

    DOE Patents [OSTI]

    Hall, Jeff G.; Lyamichev, Victor I.; Mast, Andrea L.; Brow, Mary Ann; Kwiatkowski, Robert W.; Vavra, Stephanie H.

    2005-03-29T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

  6. Invasive cleavage of nucleic acids

    DOE Patents [OSTI]

    Prudent, James R. (Madison, WI); Hall, Jeff G. (Madison, WI); Lyamichev, Victor I. (Madison, WI); Brow, Mary Ann D. (Madison, WI); Dahlberg, James E. (Madison, WI)

    2002-01-01T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

  7. Self-assembling multimeric nucleic acid constructs

    DOE Patents [OSTI]

    Cantor, Charles R. (Boston, MA); Niemeyer, Christof M. (Bremen, DE); Smith, Cassandra L. (Boston, MA); Sano, Takeshi (Boston, MA); Hnatowich, Donald J. (Brookline, MA); Rusckowski, Mary (Southborough, MA)

    1999-10-12T23:59:59.000Z

    The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

  8. Self-assembling multimeric nucleic acid constructs

    DOE Patents [OSTI]

    Cantor, Charles R. (Boston, MA); Niemeyer, Christof M. (Bremen, DE); Smith, Cassandra L. (Boston, MA); Sano, Takeshi (Boston, MA); Hnatowich, Donald J. (Brookline, MA); Rusckowski, Mary (Southborough, MA)

    1996-01-01T23:59:59.000Z

    The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

  9. Self-assembling multimeric nucleic acid constructs

    DOE Patents [OSTI]

    Cantor, C.R.; Niemeyer, C.M.; Smith, C.L.; Sano, Takeshi; Hnatowich, D.J.; Rusckowski, M.

    1996-10-01T23:59:59.000Z

    The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products. 5 figs.

  10. Double stranded nucleic acid biochips

    DOE Patents [OSTI]

    Chernov, Boris; Golova, Julia

    2006-05-23T23:59:59.000Z

    This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

  11. Chip-based sequencing nucleic acids

    DOE Patents [OSTI]

    Beer, Neil Reginald

    2014-08-26T23:59:59.000Z

    A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

  12. Replica amplification of nucleic acid arrays

    DOE Patents [OSTI]

    Church, George M. (Brookline, MA); Mitra, Robi D. (Chestnut Hill, MA)

    2010-08-31T23:59:59.000Z

    Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.

  13. Methods for analyzing nucleic acid sequences

    DOE Patents [OSTI]

    Korlach, Jonas (Ithaca, NY); Webb, Watt W. (Ithaca, NY); Levene, Michael (Ithaca, NY); Turner, Stephen (Ithaca, NY); Craighead, Harold G. (Ithaca, NY); Foquet, Mathieu (Ithaca, NY)

    2011-05-17T23:59:59.000Z

    The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.

  14. Amplification of trace amounts of nucleic acids

    DOE Patents [OSTI]

    Church, George M. (Brookline, MA); Zhang, Kun (Brighton, MA)

    2008-06-17T23:59:59.000Z

    Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).

  15. Nucleic acid based fluorescent sensor for copper detection

    DOE Patents [OSTI]

    Lu, Yi; Liu, Juewen

    2013-04-02T23:59:59.000Z

    A nucleic acid enzyme responsive to copper, comprising an oligonucleotide comprising a nucleotide sequence of SEQ ID NO:1, wherein the nucleic acid enzyme is not self-cleaving.

  16. Method for identifying and quantifying nucleic acid sequence aberrations

    DOE Patents [OSTI]

    Lucas, Joe N. (San Ramon, CA); Straume, Tore (Tracy, CA); Bogen, Kenneth T. (Walnut Creek, CA)

    1998-01-01T23:59:59.000Z

    A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. The method uses a first hybridization probe which includes a nucleic acid sequence that is complementary to a first sequence type and a first complexing agent capable of attaching to a second complexing agent and a second hybridization probe which includes a nucleic acid sequence that selectively hybridizes to the second nucleic acid sequence type over the first sequence type and includes a detectable marker for detecting the second hybridization probe.

  17. Method for nucleic acid isolation using supercritical fluids

    DOE Patents [OSTI]

    Nivens, David E. (11912 Kingsgate Rd., Knoxville, TN 37911); Applegate, Bruce M. (3700 Sutherland Ave. #Q2, Knoxville, TN 37911)

    1999-01-01T23:59:59.000Z

    A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.

  18. Nucleic acid analysis using terminal-phosphate-labeled nucleotides

    DOE Patents [OSTI]

    Korlach, Jonas (Ithaca, NY); Webb, Watt W. (Ithaca, NY); Levene, Michael (Ithaca, NY); Turner, Stephen (Ithaca, NY); Craighead, Harold G. (Ithaca, NY); Foquet, Mathieu (Ithaca, NY)

    2008-04-22T23:59:59.000Z

    The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.

  19. Detection of nucleic acids by multiple sequential invasive cleavages

    DOE Patents [OSTI]

    Hall, Jeff G; Lyamichev, Victor I; Mast, Andrea L; Brow, Mary Ann D

    2012-10-16T23:59:59.000Z

    The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

  20. Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid probes

    DOE Patents [OSTI]

    Ramsey, J. Michael (Knoxville, TN); Foote, Robert S. (Oak Ridge, TN)

    2002-01-01T23:59:59.000Z

    A method and apparatus for analyzing nucleic acids includes immobilizing nucleic probes at specific sites within a microchannel structure and moving target nucleic acids into proximity to the probes in order to allow hybridization and fluorescence detection of specific target sequences.

  1. Nucleic acids, compositions and uses thereof

    DOE Patents [OSTI]

    Preston, III, James F. (Micanopy, FL); Chow, Virginia (Gainesville, FL); Nong, Guang (Gainesville, FL); Rice, John D. (Gainesville, FL); St. John, Franz J. (Baltimore, MD)

    2012-02-21T23:59:59.000Z

    The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.

  2. Nucleic acid compositions and the encoding proteins

    DOE Patents [OSTI]

    Preston, III, James F.; Chow, Virginia; Nong, Guang; Rice, John D.; St. John, Franz J.

    2014-09-02T23:59:59.000Z

    The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.

  3. Ramon Eritja Nucleic Acid Chemistry Group

    E-Print Network [OSTI]

    Ritort, Felix

    Ramon Eritja Nucleic Acid Chemistry Group Chemistry and Molecular Pharmacology Dpt. IRB Barcelona nanoparticle assemblies. #12;The DNA can be used as template for the fabrication of silver nanowires between;Preparation of thiol-functionalized arrays N N O NH S-St Bu N N O NH S-St Bu N N O NH S-St BuO DMT-O O P O N

  4. Nucleic Acid Database: a Repository of Three-Dimensional Information about Nucleic Acids

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Berman, H. M.; Olson, W. K.; Beveridge, D. L.; Westbrook, J.; Gelbin, A.; Demeny, T.; Hsieh, S. H.; Srinivasan, A. R.; Schneider, B.

    The Nucleic Acid Database (NDB) provides 3-D structural information about nucleic acids.  It is a relational database designed to facilitate the easy search for nucleic acid structures using any of the stored primary or derived structural features. Reports can then be created describing any properties of the selected structures and structures may be viewed in several different formats, including the mmCIF format, the NDB Atlas format, the NDB coordinate format, or the PDB coordinate format. Browsing structure images created directly from coordinates in the repository can also be done. More than 7000 structures have been released as of May 2014. This website also includes a number of specialized tools and interfaces. The NDB Project is funded by the National Institutes of Health and has been funded by the National Science Foundation and the Department of Energy in the past.

  5. EGVII endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2008-11-11T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

  6. EGVII endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yao, Jian

    2014-02-25T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

  7. EGVI endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2010-10-12T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6, and the corresponding EGVI amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

  8. EGVI endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2010-10-05T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6, and the corresponding EGVI amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

  9. EGVII endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2012-02-14T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

  10. EGVII endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2009-05-05T23:59:59.000Z

    The present invention provides an endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

  11. EGVI endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2008-04-01T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6, and the corresponding EGVI amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.

  12. EGVII endoglucanase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yao, Jian

    2013-07-16T23:59:59.000Z

    The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.

  13. Nucleic acid amplification using modular branched primers

    DOE Patents [OSTI]

    Ulanovsky, Levy (Westmont, IL)

    2001-01-01T23:59:59.000Z

    Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.

  14. Nucleic acids encoding metal uptake transporters and their uses

    DOE Patents [OSTI]

    Schroeder, Julian I. (La Jolla, CA); Antosiewicz, Danuta M. (Warsaw, PL); Schachtman, Daniel P. (Tranmere, AU); Clemens, Stephan (San Diego, CA)

    1999-01-01T23:59:59.000Z

    The invention provides LCT1 nucleic acids which encode metal ion uptake transporters. The invention also provides methods of modulating heavy metal and alkali metal uptake in plants. The methods involve producing transgenic plants comprising a recombinant expression cassette containing an LCT1 nucleic acid linked to a plant promoter.

  15. Digital MDA for enumeration of total nucleic acid contamination

    E-Print Network [OSTI]

    Quake, Stephen R.

    Digital MDA for enumeration of total nucleic acid contamination Paul C. Blainey and Stephen R; Accepted October 14, 2010 ABSTRACT Multiple displacement amplification (MDA) is an iso- thermal, sequence). Here we report digital MDA (dMDA), an ultrasensitive method for quantifying nucleic acid fragments

  16. Oligonucleoside alkyl or arylphosphonate derivatives capable of crosslinking with or cleaving nucleic acids

    DOE Patents [OSTI]

    Miller, Paul S. (Baltimore, MD); Ts'o, Paul O.P. (Lutherville, MD)

    1999-06-15T23:59:59.000Z

    A composition for inactivating a target nucleic acid which comprises an oligonucleoside alkyl or arylphosphonate analogue which is complementary to the sequence of the target nucleic acid and includes a functional group which reacts with the target nucleic acid to render the target nucleic acid inactive or nonfunctional.

  17. Nucleic Acid Conformational Changes Essential for HIV-1 Nucleocapsid Protein-mediated Inhibition of

    E-Print Network [OSTI]

    Levin, Judith G.

    and a TAR-containing acceptor RNA molecule, we find that when both nucleic acids are present, NC facilitatesNucleic Acid Conformational Changes Essential for HIV-1 Nucleocapsid Protein-mediated Inhibition) is a nucleic acid chaperone protein that has been shown to greatly facilitate the nucleic acid rearrangements

  18. Oligonucleoside alkyl or arylphosphonate derivatives capable of crosslinking with or cleaving nucleic acids

    DOE Patents [OSTI]

    Miller, P.S.; Ts'o, P.O.P.

    1999-06-15T23:59:59.000Z

    A composition for inactivating a target nucleic acid which comprises an oligonucleoside alkyl or arylphosphonate analogue which is complementary to the sequence of the target nucleic acid is provided. It includes a functional group which reacts with the target nucleic acid to render the target nucleic acid inactive or nonfunctional. 16 figs.

  19. Isolated menthone reductase and nucleic acid molecules encoding same

    DOE Patents [OSTI]

    Croteau, Rodney B; Davis, Edward M; Ringer, Kerry L

    2013-04-23T23:59:59.000Z

    The present invention provides isolated menthone reductase proteins, isolated nucleic acid molecules encoding menthone reductase proteins, methods for expressing and isolating menthone reductase proteins, and transgenic plants expressing elevated levels of menthone reductase protein.

  20. Optimization of Encoded Hydrogel Particles for Nucleic Acid Quantification

    E-Print Network [OSTI]

    Pregibon, Daniel C.

    The accurate quantification of nucleic acids is of utmost importance for clinical diagnostics, drug discovery, and basic science research. These applications require the concurrent measurement of multiple targets while ...

  1. Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid

    DOE Patents [OSTI]

    Nasarabadi, Shanavaz (Livermore, CA)

    2011-01-11T23:59:59.000Z

    A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.

  2. Thermoplastic Microfluidic Device for On-Chip Purification of Nucleic Acids for Disposable

    E-Print Network [OSTI]

    Thermoplastic Microfluidic Device for On-Chip Purification of Nucleic Acids for Disposable, Brookline, Massachusetts 02446 A polymeric microfluidic device for solid-phase extraction (SPE applications. Microfluidic approaches to nucleic acid isolation have therefore received great attention

  3. Nucleic Acids Research, 2009, 112 doi:10.1093/nar/gkp675

    E-Print Network [OSTI]

    Hassibi, Arjang

    Nucleic Acids Research, 2009, 1­12 doi:10.1093/nar/gkp675 Real-time DNA microarray analysis Arjang for the analysis of complex nucleic acid samples, use the base pairing of nucleic acid molecules (3) as both uncertainty associated with tar- get analyte capturing and detection, in all practical biosensors, binding

  4. Mosaic protein and nucleic acid vaccines against hepatitis C virus

    DOE Patents [OSTI]

    Yusim, Karina; Korber, Bette T. M.; Kuiken, Carla L.; Fischer, William M.

    2013-06-11T23:59:59.000Z

    The invention relates to immunogenic compositions useful as HCV vaccines. Provided are HCV mosaic polypeptide and nucleic acid compositions which provide higher levels of T-cell epitope coverage while minimizing the occurrence of unnatural and rare epitopes compared to natural HCV polypeptides and consensus HCV sequences.

  5. Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same

    SciTech Connect (OSTI)

    Brown, Kimberly; Harris, Paul; Zaretsky, Elizabeth; Re, Edward; Vlasenko, Elena; McFarland, Keith; Lopez de Leon, Alfredo

    2010-06-22T23:59:59.000Z

    The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

  6. Cell cycle nucleic acids, polypeptides and uses thereof

    DOE Patents [OSTI]

    Gordon-Kamm, William J. (Urbandale, IA); Lowe, Keith S. (Johnston, IA); Larkins, Brian A. (Tucson, AZ); Dilkes, Brian R. (Tucson, AZ); Sun, Yuejin (Westfield, IN)

    2007-08-14T23:59:59.000Z

    The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression, cell number and/or composition of plants.

  7. Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same

    DOE Patents [OSTI]

    Brown, Kimberly; Harris, Paul; Zaretsky, Elizabeth; Re, Edward; Vlasenko, Elena; McFarland, Keith; Lopez de Leon, Alfredo

    2014-09-30T23:59:59.000Z

    The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

  8. Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same

    DOE Patents [OSTI]

    Brown, Kimberly; Harris, Paul; Zaretsky, Elizabeth; Re, Edward; Vlasenko, Elena; McFarland, Keith; Lopez de Leon, Alfredo

    2012-10-16T23:59:59.000Z

    The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

  9. Isolated nucleic acids encoding antipathogenic polypeptides and uses thereof

    DOE Patents [OSTI]

    Altier, Daniel J.; Crane, Virginia C.; Ellanskaya, Irina; Ellanskaya, Natalia; Gilliam, Jacob T.; Hunter-Cevera, Jennie; Presnail, James K.; Schepers, Eric J.; Simmons, Carl R.; Torok, Tamas; Yalpani, Nasser

    2010-04-20T23:59:59.000Z

    Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

  10. Nucleic acids encoding antifungal polypeptides and uses thereof

    DOE Patents [OSTI]

    Altier, Daniel J. (Granger, IA); Ellanskaya, I. A. (Kyiv, UA); Gilliam, Jacob T. (Norwalk, IA); Hunter-Cevera, Jennie (Elliott City, MD); Presnail, James K (Avondale, PA); Schepers, Eric (Port Deposit, MD); Simmons, Carl R. (Des Moines, IA); Torok, Tamas (Richmond, CA); Yalpani, Nasser (Johnston, IA)

    2010-11-02T23:59:59.000Z

    Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

  11. Method and apparatus for staining immobilized nucleic acids

    DOE Patents [OSTI]

    Ramsey, J. Michael (Knoxville, TN); Foote, Robert S. (Oak Ridge, TN); Jacobson, Stephen C. (Knoxville, TN)

    2000-01-01T23:59:59.000Z

    A method for staining immobilized nucleic acids includes the steps of affixing DNA probes to a solid substrate, moving target DNA material into proximity with the DNA probes, whereby the target DNA hybridized with specific ones of the DNA probes, and moving a fluorescent dye into proximity with the hybridized target DNA, whereby the fluorescent dye binds to the hybridized DNA to enable subsequent detection of fluorescence.

  12. Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions

    DOE Patents [OSTI]

    Lucas, Joe N. (San Ramon, CA); Straume, Tore (Tracy, CA); Bogen, Kenneth T. (Walnut Creek, CA)

    1998-01-01T23:59:59.000Z

    A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration.

  13. Nucleic acid modifications in bacterial pathogens - impact on pathogenesis, diagnosis, and therapy

    E-Print Network [OSTI]

    Russell, Brandon S. (Brandon Skylur)

    2014-01-01T23:59:59.000Z

    Nucleic acids are subject to extensive chemical modification by all organisms. These modifications display incredible structural diversity, and some are essential for survival. Intriguingly, several of these modifications ...

  14. BGL6 .beta.-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Ward, Michael

    2012-10-02T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.

  15. BGL6 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Ward, Michael

    2014-03-04T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.

  16. BGL7 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Ward, Michael

    2014-03-25T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.

  17. BGL7 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Ward, Michael

    2013-01-29T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.

  18. BGL6 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Ward, Michael (San Francisco, CA)

    2009-09-01T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl6, and the corresponding BGL6 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL6, recombinant BGL6 proteins and methods for producing the same.

  19. BGL3 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2008-04-01T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

  20. BGL3 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2011-06-14T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

  1. BGL3 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yao, Jian

    2012-10-30T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

  2. BGL4 .beta.-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yao, Jian

    2006-05-16T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.

  3. BGL5 .beta.-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2008-03-18T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl5, and the corresponding BGL5 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL5, recombinant BGL5 proteins and methods for producing the same.

  4. BGL4 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA) [Los Gatos, CA; Goedegebuur, Frits (Vlaardingen, NL) [Vlaardingen, NL; Ward, Michael (San Francisco, CA) [San Francisco, CA; Yao, Jian (Sunnyvale, CA) [Sunnyvale, CA

    2008-01-22T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.

  5. BGL5 .beta.-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel; Goedegebuur, Frits; Ward, Michael; Yao, Jian

    2006-02-28T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl5, and the corresponding BGL5 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL5, recombinant BGL5 proteins and methods for producing the same.

  6. BGL7 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Ward, Michael (San Francisco, CA)

    2008-08-05T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.

  7. BGL3 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2007-09-25T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

  8. BGL4 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Goedegebuur, Frits (Vlaardingen, NL); Ward, Michael (San Francisco, CA); Yao, Jian (Sunnyvale, CA)

    2011-12-06T23:59:59.000Z

    The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.

  9. Nucleic acids encoding human trithorax protein

    DOE Patents [OSTI]

    Evans, Glen A. (Encinitas, CA); Djabali, Malek (Marseilles, FR); Selleri, Licia (Del Mar, CA); Parry, Pauline (San Diego, CA)

    2001-01-01T23:59:59.000Z

    In accordance with the present invention, there is provided an isolated peptide having the characteristics of human trithorax protein (as well as DNA encoding same, antisense DNA derived therefrom and antagonists therefor). The invention peptide is characterized by having a DNA binding domain comprising multiple zinc fingers and at least 40% amino acid identity with respect to the DNA binding domain of Drosophila trithorax protein and at least 70% conserved sequence with respect to the DNA binding domain of Drosophila trithorax protein, and wherein said peptide is encoded by a gene located at chromosome 11 of the human genome at q23. Also provided are methods for the treatment of subject(s) suffering from immunodeficiency, developmental abnormality, inherited disease, or cancer by administering to said subject a therapeutically effective amount of one of the above-described agents (i.e., peptide, antagonist therefor, DNA encoding said peptide or antisense DNA derived therefrom). Also provided is a method for the diagnosis, in a subject, of immunodeficiency, developmental abnormality, inherited disease, or cancer associated with disruption of chromosome 11 at q23.

  10. Structure and function of circadian clock proteins and deuterium isotope effects in nucleic acid hydrogen bonds

    E-Print Network [OSTI]

    Vakonakis, Ioannis

    2005-08-29T23:59:59.000Z

    -terminal domain. Hydrogen bonds are of paramount importance in nucleic acid structure and function. Here we show that changes in the width and anharmonicity of vibrational potential energy wells of hydrogen bonded groups can be measured in nucleic acids and can...

  11. A Standard Reference Frame for the Description of Nucleic Acid Base-pair Geometry

    E-Print Network [OSTI]

    Gerstein, Mark

    uncertainties in this data set closely match numerical values reported in the recent survey of nucleic acid baseA Standard Reference Frame for the Description of Nucleic Acid Base-pair Geometry These preliminary (Rockefeller University), Richard E. Dickerson (University of California, Los Angeles), Mark Gerstein (Yale

  12. Electrochemical control of reversible DNA hybridisation : for future use in nucleic acid amplification 

    E-Print Network [OSTI]

    Syed, Shahida Nina

    2014-06-28T23:59:59.000Z

    Denaturation and renaturation is indispensable for the biological function of nucleic acids in many cellular processes, such as for example transcription for the synthesis of RNA and DNA replication during cell division. ...

  13. Development of polymer and lipid materials for enhanced delivery of nucleic acids and proteins

    E-Print Network [OSTI]

    Eltoukhy, Ahmed Atef

    2013-01-01T23:59:59.000Z

    The development of synthetic vectors enabling efficient intracellular delivery of macromolecular therapeutics such as nucleic acids and proteins could potentially catalyze the clinical translation of many gene and protein-based ...

  14. Two-dimensional infrared spectroscopy of nucleic acids : application to tautomerism and DNA aptamer unfolding dynamics

    E-Print Network [OSTI]

    Peng, Chunte Sam

    2014-01-01T23:59:59.000Z

    The structural dynamics of nucleic acids are intimately related to their biological functions; however, our ability to study these molecular dynamics has been largely impeded by the lack of techniques that possess both ...

  15. Plants having modified response to ethylene by transformation with an ETR nucleic acid

    DOE Patents [OSTI]

    Meyerowitz, Elliott M. (Pasadena, CA); Chang, Caren (Pasadena, CA); Bleecker, Anthony B. (Madison, WI)

    2001-01-01T23:59:59.000Z

    The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic acid is controlled by selecting appropriate expression regulation sequences to target the location and/or time of expression of the transformed nucleic acid. The plants are made by transforming at least one plant cell with an appropriate modified ETR nucleic acid, regenerating plants from one or more of the transformed plant cells and selecting at least one plant having the desired phenotype.

  16. Studies toward biomimetic claisen condensation using nucleic acid templates and ribozyme catalysis

    E-Print Network [OSTI]

    Ryu, Youngha

    2005-08-29T23:59:59.000Z

    Many different experimental approaches were attempted to achieve carbon-carbon bond formation by nucleic acid template-directed reactions and ribozyme catalysis as potential lipid synthesizing machineries in the RNA world. A novel biomimetic...

  17. Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe

    DOE Patents [OSTI]

    Lucas, Joe N. (San Ramon, CA); Straume, Tore (Tracy, CA); Bogen, Kenneth T. (Walnut Creek, CA)

    2000-01-01T23:59:59.000Z

    A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.

  18. Detection and isolation of nucleic acid sequences using competitive hybridization probes

    DOE Patents [OSTI]

    Lucas, J.N.; Straume, T.; Bogen, K.T.

    1997-04-01T23:59:59.000Z

    A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent, the second complexing agent being attached to a solid support such that when the first and second complexing agents are attached, target nucleic acid sequences hybridized to the first hybridization probe become immobilized on to the solid support. The immobilized target nucleic acids are then separated and detected by detecting the detectable marker attached to the second hybridization probe. A kit for performing the method is also provided. 7 figs.

  19. Method for producing labeled single-stranded nucleic acid probes

    DOE Patents [OSTI]

    Dunn, John J. (Bellport, NY); Quesada, Mark A. (Middle Island, NY); Randesi, Matthew (Upton, NY)

    1999-10-19T23:59:59.000Z

    Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment. More specifically, the method comprises providing a recombinant DNA construct comprising a DNA segment of interest inserted in a cloning vector, the cloning vector having an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment of interest. The recombinant DNA construct is then contacted with the protein pII encoded by gene II of phage f1 thereby generating a single-stranded nick. The nicked DNA is then contacted with E. coli Exonuclease III thereby expanding the single-stranded nick into a single-stranded gap. The single-stranded gapped DNA is then contacted with a single-strand-specific endonuclease thereby producing a linearized DNA molecule containing a double-stranded deletion corresponding in size to the single-stranded gap. The DNA treated in this manner is then incubated with DNA ligase under conditions appropriate for ligation. Also disclosed is a method for producing single-stranded DNA probes. In this embodiment, single-stranded gapped DNA, produced as described above, is contacted with a DNA polymerase in the presence of labeled nucleotides to fill in the gap. This DNA is then linearized by digestion with a restriction enzyme which cuts outside the DNA segment of interest. The product of this digestion is then denatured to produce a labeled single-stranded nucleic acid probe.

  20. DimaSense™: A Novel Nucleic Acid Detection System

    SciTech Connect (OSTI)

    Stadler, A.

    2011-05-18T23:59:59.000Z

    Recently, we developed a suite of methods for the rational design and fabrication of well-defined nanoparticle architectures, including clusters using bio-encoded nanoscale building blocks and layer-by-layer stepwise assembly on a solid support. In particular, the Nano-Assembly platform using Encoded Solid Supports (NAESS) allows for controlled interactions, purification of side products, modularity of design, and the construction of complex nanoparticle architectures. This approach offers several advantages over the current art of designing nanoparticle clusters, which include the high-yield synthesis of desired architectures, a 'plug-and-play' design allowing for the introduction of a variety of sensing modalities, and ease of scalability in high-throughput and synthesis yield. As a utility proof of concept, we implemented our unique cluster fabrication platform to design gold nanoparticle dimers which are linked via a single-stranded DNA oligonucleotide recognition motif. The design of this motif is such that binding of complementary nucleic acids results in specific, selective and rapid dimer dissociation, which can be monitored by dynamic light scattering (DLS). We demonstrated single level mismatch selectivity using this approach. The limit of detection was determined to be 1011 molecules of synthetic target RNA or DNA within 30 minutes of incubation at 33 C. This detection limit is determined by the dimer's concentration which can be probed by currently used standard DLS instruments. We also demonstrated a specific detection of target RNA in a solution containing competing 1,000-fold excess of non-complementary DNA fragments, 10% BSA, and endonucleases. Molecular diagnostic companies, RNA-based technology developers, and personalized medicine companies have applications that could benefit from using DimaSense{trademark}. The technology represents a platform which enables the simple and reasonably inexpensive design and fabrication of highly selective genetic sensors. These sensors operate with very low concentrations of target, can utilize standard instrumentation, produce detection results rapidly, and are robust enough to function in the presence of many competing genetic targets. Many current genetic target detection products/approaches/technologies rely upon methods (such as qPCR) which are more complicated, cumbersome, and costly to perform, and are not well suited to point-of-care diagnostic applications. Several clinical diagnostic applications, particularly point-of-care (POC) diagnostics for infectious diseases, are possible and appear to be a good fit for the technology. In addition, the advent of personalized medicine will create opportunities for molecular diagnostic companies with the capabilities of rapidly and quantitatively detecting nucleic acid sequences. The global POC market was {approx}$7.7B in 2010, with a recent annual growth rate of {approx}7%. A specific disease or disease-class diagnostic would need to be identified before a more meaningful sub-market value could be stated. Additional validation of the technology to show that it displays appropriate performance parameters for a commercial application on 'real world' samples is required for true commercial readiness. In addition, optimization of sensor design parameters, to effect a 10-fold increase in sensitivity, may be required to produce a commercially ready sensor system. These validation and sensor design optimization are estimated to require 3-4 months and {approx}$75k. For an unregulated product to give this sensor system a distinct competitive advantage, 2-3 years of product development and $1.5-3M are likely required. For regulated markets, time to market (through clinic) and cost would depend upon the product.

  1. Methods of staining target chromosomal DNA employing high complexity nucleic acid probes

    DOE Patents [OSTI]

    Gray, Joe W.; Pinkel, Daniel; Kallioniemi, Ol'li-Pekka; Kallioniemi, Anne; Sakamoto, Masaru

    2006-10-03T23:59:59.000Z

    Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

  2. Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

    DOE Patents [OSTI]

    Kwok, Pui-Yan (Clayton, MO); Chen, Xiangning (St. Louis, MO)

    1999-01-01T23:59:59.000Z

    A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

  3. Structural aspects of catalytic mechanisms of endonucleases and their binding to nucleic acids

    SciTech Connect (OSTI)

    Zhukhlistova, N. E.; Balaev, V. V.; Lyashenko, A. V.; Lashkov, A. A., E-mail: alashkov83@gmail.com [Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)

    2012-05-15T23:59:59.000Z

    Endonucleases (EC 3.1) are enzymes of the hydrolase class that catalyze the hydrolytic cleavage of deoxyribonucleic and ribonucleic acids at any region of the polynucleotide chain. Endonucleases are widely used both in biotechnological processes and in veterinary medicine as antiviral agents. Medical applications of endonucleases in human cancer therapy hold promise. The results of X-ray diffraction studies of the spatial organization of endonucleases and their complexes and the mechanism of their action are analyzed and generalized. An analysis of the structural studies of this class of enzymes showed that the specific binding of enzymes to nucleic acids is characterized by interactions with nitrogen bases and the nucleotide backbone, whereas the nonspecific binding of enzymes is generally characterized by interactions only with the nucleic-acid backbone. It should be taken into account that the specificity can be modulated by metal ions and certain low-molecular-weight organic compounds. To test the hypotheses about specific and nonspecific nucleic-acid-binding proteins, it is necessary to perform additional studies of atomic-resolution three-dimensional structures of enzyme-nucleic-acid complexes by methods of structural biology.

  4. Nucleic Acids Research, 2009, 114 doi:10.1093/nar/gkn1064

    E-Print Network [OSTI]

    Shamir, Ron

    Nucleic Acids Research, 2009, 1­14 doi:10.1093/nar/gkn1064 Allegro: Analyzing expression- ulate. We present Allegro, a method for de-novo dis- covery of cis-regulatory transcriptional programs by a group of co-regulated genes. We show that Allegro is more accurate and sensitive than existing

  5. Adsorption of Nucleic Acid Components on Rutile (TiO2) Surfaces

    E-Print Network [OSTI]

    Sverjensky, Dimitri A.

    Adsorption of Nucleic Acid Components on Rutile (TiO2) Surfaces H. James Cleaves II,1 Caroline M and horizontal gene transfer. The adsorption of mono-, oligo-, and polynucleotides and their components obtained from studies of other minerals. In contrast with recent studies of nucleotide adsorption on Zn

  6. Do Electrostatic Interactions Destabilize ProteinNucleic Acid Binding? Sanbo Qin,1

    E-Print Network [OSTI]

    Weston, Ken

    interaction energies between proteins and nucleic acids are positive, meaning that electrostatic interactions as the boundary between the solute low dielectric and the solvent dielectric. We have explored an alternative choice, i.e., the van der Waals (vdW) surface, and found that the electrostatic interaction energy

  7. Nucleosides, Nucleotides, and Nucleic Acids, 25:915, 2006 Copyright C Taylor & Francis Group, LLC

    E-Print Network [OSTI]

    Stoltz, Brian M.

    Nucleosides, Nucleotides, and Nucleic Acids, 25:9­15, 2006 Copyright C Taylor & Francis Group, LLC-LABELED NUCLEOTIDE SPACER ARM ON INCORPORATION BY THERMOPHILIC DNA POLYMERASES Christopher J. Lacenere 2 Division incor- porate fluorescently labeled nucleotides sequentially was analyzed by a gel based primer

  8. 1997 Oxford University Press49945002 Nucleic Acids Research, 1997, Vol. 25, No. 24 Information analysis of Fis binding sites

    E-Print Network [OSTI]

    Schneider, Thomas D.

    © 1997 Oxford University Press4994­5002 Nucleic Acids Research, 1997, Vol. 25, No. 24 Information to fit into two successive major grooves on straight B-form DNA, suggesting that the DNA bends

  9. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14T23:59:59.000Z

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  10. Nucleic Acid Encoding A Lectin-Derived Progenitor Cell Preservation Factor

    DOE Patents [OSTI]

    Colucci, M. Gabriella (Dugenta, IT); Chrispeels, Maarten J. (La Jolla, CA); Moore, Jeffrey G. (New York, NY)

    2001-10-30T23:59:59.000Z

    The invention relates to an isolated nucleic acid molecule that encodes a protein that is effective to preserve progenitor cells, such as hematopoietic progenitor cells. The nucleic acid comprises a sequence defined by SEQ ID NO:1, a homolog thereof, or a fragment thereof. The encoded protein has an amino acid sequence that comprises a sequence defined by SEQ ID NO:2, a homolog thereof, or a fragment thereof that contains an amino acid sequence TNNVLQVT. Methods of using the encoded protein for preserving progenitor cells in vitro, ex vivo, and in vivo are also described. The invention, therefore, include methods such as myeloablation therapies for cancer treatment wherein myeloid reconstitution is facilitated by means of the specified protein. Other therapeutic utilities are also enabled through the invention, for example, expanding progenitor cell populations ex vivo to increase chances of engraftation, improving conditions for transporting and storing progenitor cells, and facilitating gene therapy to treat and cure a broad range of life-threatening hematologic diseases.

  11. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides

    DOE Patents [OSTI]

    Studier, F. William (Stony Brook, NY)

    1995-04-18T23:59:59.000Z

    Random and directed priming methods for determining nucleotide sequences by enzymatic sequencing techniques, using libraries of primers of lengths 8, 9 or 10 bases, are disclosed. These methods permit direct sequencing of nucleic acids as large as 45,000 base pairs or larger without the necessity for subcloning. Individual primers are used repeatedly to prime sequence reactions in many different nucleic acid molecules. Libraries containing as few as 10,000 octamers, 14,200 nonamers, or 44,000 decamers would have the capacity to determine the sequence of almost any cosmid DNA. Random priming with a fixed set of primers from a smaller library can also be used to initiate the sequencing of individual nucleic acid molecules, with the sequence being completed by directed priming with primers from the library. In contrast to random cloning techniques, a combined random and directed priming strategy is far more efficient.

  12. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides

    DOE Patents [OSTI]

    Studier, F.W.

    1995-04-18T23:59:59.000Z

    Random and directed priming methods for determining nucleotide sequences by enzymatic sequencing techniques, using libraries of primers of lengths 8, 9 or 10 bases, are disclosed. These methods permit direct sequencing of nucleic acids as large as 45,000 base pairs or larger without the necessity for subcloning. Individual primers are used repeatedly to prime sequence reactions in many different nucleic acid molecules. Libraries containing as few as 10,000 octamers, 14,200 nonamers, or 44,000 decamers would have the capacity to determine the sequence of almost any cosmid DNA. Random priming with a fixed set of primers from a smaller library can also be used to initiate the sequencing of individual nucleic acid molecules, with the sequence being completed by directed priming with primers from the library. In contrast to random cloning techniques, a combined random and directed priming strategy is far more efficient. 2 figs.

  13. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

    DOE Patents [OSTI]

    Thompson, David N; Apel, William A; Thompson, Vicki S; Ward, Thomas E

    2013-07-23T23:59:59.000Z

    A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

  14. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes

    DOE Patents [OSTI]

    Thompson, David N; Apel, William A; Thompson, Vicki S; Ward, Thomas E

    2014-04-08T23:59:59.000Z

    A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.

  15. Alteration of Nucleic Acid Structure and Stability Modulates the Efficiency of Minus-Strand Transfer Mediated by the

    E-Print Network [OSTI]

    Levin, Judith G.

    Alteration of Nucleic Acid Structure and Stability Modulates the Efficiency of Minus destabilizes the highly structured complementary trans-activation response ele- ment (TAR) stem-loop (TAR DNA) at the 3 -end of ( ) SSDNA and inhibits TAR-induced self-priming, a dead- end reaction that competes

  16. Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"

    SciTech Connect (OSTI)

    Stadler, A.L.; van der Lelie, D.; Sun, D.; Maye, M. M.; Gang, O.

    2011-04-01T23:59:59.000Z

    We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

  17. Quantification of false positive reduction in nucleic acid purification on hemorrhagic fever DNA.

    SciTech Connect (OSTI)

    James, Conrad D.; Pohl, Kenneth Roy; Derzon, Mark Steven; McClain, Jaime; Achyuthan, Komandoor

    2006-11-01T23:59:59.000Z

    Columbia University has developed a sensitive highly multiplexed system for genetic identification of nucleic acid targets. The primary obstacle to implementing this technology is the high rate of false positives due to high levels of unbound reporters that remain within the system after hybridization. The ability to distinguish between free reporters and reporters bound to targets limits the use of this technology. We previously demonstrated a new electrokinetic method for binary separation of kb pair long DNA molecules and oligonucleotides. The purpose of this project 99864 is to take these previous demonstrations and further develop the technique and hardware for field use. Specifically, our objective was to implement separation in a heterogeneous sample (containing target DNA and background oligo), to perform the separation in a flow-based device, and to develop all of the components necessary for field testing a breadboard prototype system.

  18. Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes

    DOE Patents [OSTI]

    Lu, Yi (Champaign, IL); Liu, Juewen (Albuquerque, NM)

    2011-11-15T23:59:59.000Z

    The present invention provides aptamer- and nucleic acid enzyme-based systems for simultaneously determining the presence and optionally the concentration of multiple analytes in a sample. Methods of utilizing the system and kits that include the sensor components are also provided. The system includes a first reactive polynucleotide that reacts to a first analyte; a second reactive polynucleotide that reacts to a second analyte; a third polynucleotide; a fourth polynucleotide; a first particle, coupled to the third polynucleotide; a second particle, coupled to the fourth polynucleotide; and at least one quencher, for quenching emissions of the first and second quantum dots, coupled to the first and second reactive polynucleotides. The first particle includes a quantum dot having a first emission wavelength. The second particle includes a second quantum dot having a second emission wavelength different from the first emission wavelength. The third polynucleotide and the fourth polynucleotide are different.

  19. 1996 Oxford University Press 47094718Nucleic Acids Research, 1996, Vol. 24, No. 23 Logitlinear models for the prediction of splice sites in

    E-Print Network [OSTI]

    Brendel, Volker

    © 1996 Oxford University Press 4709­4718Nucleic Acids Research, 1996, Vol. 24, No. 23 Logitlinear the degree of fit to some average signal pattern around known splice sites in a learning set (e.g., 3

  20. Human retroviruses and AIDS 1996. A compilation and analysis of nucleic acid and amino acid sequences

    SciTech Connect (OSTI)

    Myers, G.; Foley, B.; Korber, B. [eds.] [Los Alamos National Lab., NM (United States). Theoretical Div.] [eds.; Los Alamos National Lab., NM (United States). Theoretical Div.; Mellors, J.W. [ed.] [Univ. of Pittsburgh, PA (United States)] [ed.; Univ. of Pittsburgh, PA (United States); Jeang, K.T. [ed.] [National Institutes of Health, Bethesda, MD (United States). Molecular Virology Section] [ed.; National Institutes of Health, Bethesda, MD (United States). Molecular Virology Section; Wain-Hobson, S. [Pasteur Inst., Paris (France)] [ed.] [Pasteur Inst., Paris (France); ed.

    1997-04-01T23:59:59.000Z

    This compendium and the accompanying floppy diskettes are the result of an effort to compile and rapidly publish all relevant molecular data concerning the human immunodeficiency viruses (HIV) and related retroviruses. The scope of the compendium and database is best summarized by the five parts that it comprises: (1) Nuclear Acid Alignments and Sequences; (2) Amino Acid Alignments; (3) Analysis; (4) Related Sequences; and (5) Database Communications. Information within all the parts is updated throughout the year on the Web site, http://hiv-web.lanl.gov. While this publication could take the form of a review or sequence monograph, it is not so conceived. Instead, the literature from which the database is derived has simply been summarized and some elementary computational analyses have been performed upon the data. Interpretation and commentary have been avoided insofar as possible so that the reader can form his or her own judgments concerning the complex information. In addition to the general descriptions of the parts of the compendium, the user should read the individual introductions for each part.

  1. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report

    SciTech Connect (OSTI)

    Kingsley, Mark T.

    2001-03-13T23:59:59.000Z

    The threat to American interests from terrorists is not limited to attacks against humans. Terrorists might seek to inflict damage to the U.S. economy by attacking our agricultural sector. Infection of commodity crops by bacterial or fungal crop pathogens could adversely impact U.S. agriculture, either directly from damage to crops or indirectly from damage to our ability to export crops suspected of contamination. Recognizing a terrorist attack against U.S. agriculture, to be able to prosecute the terrorists, is among the responsibilities of the members of Hazardous Material Response Unit (HMRU) of the Federal Bureau of Investigation (FBI). Nucleic acid analysis of plant pathogen strains by the use of polymerase chain reaction (PCR) amplification techniques is a powerful method for determining the exact identity of pathogens, as well as their possible region of origin. This type of analysis, however, requires that PCR assays be developed specific to each particular pathogen strain, and analysis protocols developed that are specific to the particular instrument used for detection. The objectives of the work described here were threefold: 1) to assess the potential terrorist threat to U.S. agricultural crops, 2) to determine whether suitable assays exist to monitor that threat, and 3) where assays are needed for priority plant pathogen threats, to modify or develop those assays for use by specialists at the HMRU. The assessment of potential threat to U.S. commodity crops and the availability of assays for those threats were described in detail in the Technical Requirements Document (9) and will be summarized in this report. This report addresses development of specific assays identified in the Technical Requirements Document, and offers recommendations for future development to ensure that HMRU specialists will be prepared with the PCR assays they need to protect against the threat of economic terrorism.

  2. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report

    SciTech Connect (OSTI)

    Kingsley, Mark T

    2001-03-13T23:59:59.000Z

    The threat to American interests from terrorists is not limited to attacks against humans. Terrorists might seek to inflict damage to the U.S. economy by attacking our agricultural sector. Infection of commodity crops by bacterial or fungal crop pathogens could adversely impact U.S. agriculture, either directly from damage to crops or indirectly from damage to our ability to export crops suspected of contamination. Recognizing a terrorist attack against U.S. agriculture, to be able to prosecute the terrorists, is among the responsibilities of the members of Hazardous Material Response Unit (HMRU) of the Federal Bureau of Investigation (FBI). Nucleic acid analysis of plant pathogen strains by the use of polymerase chain reaction (PCR) amplification techniques is a powerful method for determining the exact identity of pathogens, as well as their possible region of origin. This type of analysis, however, requires that PCR assays be developed specific to each particular pathogen strain, an d analysis protocols developed that are specific to the particular instrument used for detection. The objectives of the work described here were threefold: (1) to assess the potential terrorist threat to U.S. agricultural crops, (2) to determine whether suitable assays exist to monitor that threat, and (3) where assays are needed for priority plant pathogen threats, to modify or develop those assays for use by specialists at the HMRU. The assessment of potential threat to U.S. commodity crops and the availability of assays for those threats were described in detail in the Technical Requirements Document (9) and will be summarized in this report. This report addresses development of specific assays identified in the Technical Requirements Document, and offers recommendations for future development to ensure that HMRU specialists will be prepared with the PCR assays they need to protect against the threat of economic terrorism.

  3. Nucleic Acids Research doi:10.1093/nar/gkn305

    E-Print Network [OSTI]

    Lin, Guohui

    server for rapidly generating accurate 3D protein structures using only assigned nuclear magnetic no NOE and/or J-coupling data to perform its calculations. CS23D accepts chemical shift files in either. Tests conducted on more than 100 proteins from the BioMagResBank indicate that CS23D converges (i

  4. Published online 17 April 2014 Nucleic Acids Research, 2014, Vol. 42, No. 10 e83 doi: 10.1093/nar/gku250

    E-Print Network [OSTI]

    Published online 17 April 2014 Nucleic Acids Research, 2014, Vol. 42, No. 10 e83 doi: 10.1093/nar onto either magnetic beads or gold nanoparticles grafted with the com- plementary ODN, as shown variety of applications, among which is the indirect functionalization of the MPs they bind to (3

  5. Preparation of Nucleic Acid Libraries for Personalized Sequencing Systems Using an Integrated Microfluidic Hub Technology (Seventh Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting 2012)

    ScienceCinema (OSTI)

    Patel, Kamlesh D [Ken]; SNL,

    2013-01-25T23:59:59.000Z

    Kamlesh (Ken) Patel from Sandia National Laboratories (Livermore, California) presents "Preparation of Nucleic Acid Libraries for Personalized Sequencing Systems Using an Integrated Microfluidic Hub Technology " at the 7th Annual Sequencing, Finishing, Analysis in the Future (SFAF) Meeting held in June, 2012 in Santa Fe, NM.

  6. A Highly Salt-Dependent Enthalpy Change for Escherichia coli SSB Protein-Nucleic Acid Binding Due to Ion-Protein Interactions

    E-Print Network [OSTI]

    Lohman, Timothy M.

    A Highly Salt-Dependent Enthalpy Change for Escherichia coli SSB Protein-Nucleic Acid Binding Due ReceiVed February 5, 1996X ABSTRACT: We have examined the linkage between salt concentration association constant, Kobs, decreases with increasing salt concentration at all temperatures examined

  7. Nucleic acid sequences encoding D1 and D1/D2 domains of human coxsackievirus and adenovirus receptor (CAR)

    DOE Patents [OSTI]

    Freimuth, Paul I.

    2010-04-06T23:59:59.000Z

    The invention provides recombinant human CAR (coxsackievirus and adenovirus receptor) polypeptides which bind adenovirus. Specifically, polypeptides corresponding to adenovirus binding domain D1 and the entire extracellular domain of human CAR protein comprising D1 and D2 are provided. In another aspect, the invention provides nucleic acid sequences encoding these domains and expression vectors for producing the domains and bacterial cells containing such vectors. The invention also includes an isolated fusion protein comprised of the D1 polypeptide fused to a polypeptide which facilitates folding of D1 when expressed in bacteria. The functional D1 domain finds application in a therapeutic method for treating a patient infected with a CAR D1-binding virus, and also in a method for identifying an antiviral compound which interferes with viral attachment. The invention also provides a method for specifically targeting a cell for infection by a virus which binds to D1.

  8. Production of extracellular nucleic acids by genetically altered bacteria in aquatic-environment microcosms. [Escherichia coli, Pseudomonas aeroginosa, Pseudomonas cepacia, Bradyrhizobium japonicum

    SciTech Connect (OSTI)

    Paul, J.H.; David, A.W. (Univ. of South Florida, St. Petersburg (USA))

    1989-08-01T23:59:59.000Z

    The factors which affect the production of extracellular DNA by genetically altered strains of Escherichia coli, Pseudomonas aeruginosa, Pseudomonas cepacia, and Bradyrhizobium japonicum in aquatic environments were investigated. Cellular nucleic acids were labeled in vivo by incubation with ({sup 3}H)thymidine or ({sup 3}H)adenine, and production of extracellular DNA in marine waters, artificial seawater, or minimal salts media was determined by detecting radiolabeled macromolecules in incubation filtrates. The presence or absence of the ambient microbial community had little effect on the production of extracellular DNA. Three of four organisms produced the greatest amounts of extracellular nucleic acids when incubated in low-salinity media (2% artificial seawater) rather than high-salinity media (10 to 50% artificial seawater). The greatest production of extracellular nucleic acids by P. cepacia occurred at pH 7 and 37{degree}C, suggesting that extracellular-DNA production may be a normal physiologic function of the cell. Incubation of labeled P. cepacia cells in water from Bimini Harbor, Bahamas, resulted in labeling of macromolecules of the ambient microbial population. Collectively these results indicate that (i) extracellular-DNA production by genetically altered bacteria released into aquatic environments is more strongly influenced by physicochemical factors than biotic factors, (ii) extracellular-DNA production rates are usually greater for organisms released in freshwater than marine environments, and (iii) ambient microbial populations can readily utilize materials released by these organisms.

  9. Accurate small and wide angle x-ray scattering profiles from atomic models of proteins and nucleic acids

    SciTech Connect (OSTI)

    Nguyen, Hung T. [BioMaPS Institute for Quantitative Biology, Rutgers University, Piscataway, New Jersey 08854 (United States); Pabit, Suzette A.; Meisburger, Steve P.; Pollack, Lois [School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 (United States); Case, David A., E-mail: case@biomaps.rutgers.edu [BioMaPS Institute for Quantitative Biology, Rutgers University, Piscataway, New Jersey 08854 (United States); Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, New Jersey 08854 (United States)

    2014-12-14T23:59:59.000Z

    A new method is introduced to compute X-ray solution scattering profiles from atomic models of macromolecules. The three-dimensional version of the Reference Interaction Site Model (RISM) from liquid-state statistical mechanics is employed to compute the solvent distribution around the solute, including both water and ions. X-ray scattering profiles are computed from this distribution together with the solute geometry. We describe an efficient procedure for performing this calculation employing a Lebedev grid for the angular averaging. The intensity profiles (which involve no adjustable parameters) match experiment and molecular dynamics simulations up to wide angle for two proteins (lysozyme and myoglobin) in water, as well as the small-angle profiles for a dozen biomolecules taken from the BioIsis.net database. The RISM model is especially well-suited for studies of nucleic acids in salt solution. Use of fiber-diffraction models for the structure of duplex DNA in solution yields close agreement with the observed scattering profiles in both the small and wide angle scattering (SAXS and WAXS) regimes. In addition, computed profiles of anomalous SAXS signals (for Rb{sup +} and Sr{sup 2+}) emphasize the ionic contribution to scattering and are in reasonable agreement with experiment. In cases where an absolute calibration of the experimental data at q = 0 is available, one can extract a count of the excess number of waters and ions; computed values depend on the closure that is assumed in the solution of the Ornstein–Zernike equations, with results from the Kovalenko–Hirata closure being closest to experiment for the cases studied here.

  10. Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use

    DOE Patents [OSTI]

    Croteau, Rodney B. (Pullman, WA); Lange, Bernd M. (Pullman, WA)

    2001-01-01T23:59:59.000Z

    A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.

  11. Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof

    DOE Patents [OSTI]

    Croteau, Rodney Bruce (Pullman, WA); Wildung, Mark Raymond (Colfax, WA); Lange, Bernd Markus (Pullman, WA); McCaskill, David G. (Pullman, WA)

    2001-01-01T23:59:59.000Z

    cDNAs encoding 1-deoxyxylulose-5-phosphate synthase from peppermint (Mentha piperita) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7) are provided which code for the expression of 1-deoxyxylulose-5-phosphate synthase from plants. In another aspect the present invention provides for isolated, recombinant DXPS proteins, such as the proteins having the sequences set forth in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8. In other aspects, replicable recombinant cloning vehicles are provided which code for plant 1-deoxyxylulose-5-phosphate synthases, or for a base sequence sufficiently complementary to at least a portion of 1-deoxyxylulose-5-phosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a plant 1-deoxyxylulose-5-phosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant 1-deoxyxylulose-5-phosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant 1-deoxyxylulose-5-phosphate synthase may be used to obtain expression or enhanced expression of 1-deoxyxylulose-5-phosphate synthase in plants in order to enhance the production of 1-deoxyxylulose-5-phosphate, or its derivatives such as isopentenyl diphosphate (BP), or may be otherwise employed for the regulation or expression of 1-deoxyxylulose-5-phosphate synthase, or the production of its products.

  12. RES Oklahoma

    Broader source: Energy.gov [DOE]

    RES Oklahoma will feature respected tribal leaders, state, and local elected officials and top CEOs, networking, teaming opportunities, business development sessions, American Indian Procurement...

  13. RES Wisconsin

    Office of Energy Efficiency and Renewable Energy (EERE)

    The National Center for American Indian Enterprise Development (The National Center) is proud to announce RES Wisconsin, which will be held October 6th – 9th, 2014 at the Potawatomi Hotel & Casino in Milwaukee, Wisconsin.

  14. Synthesis, Improved Antisense Activity and Structural Rationale for the Divergent RNA Affinities of 3;#8242;-Fluoro Hexitol Nucleic Acid (FHNA and Ara-FHNA) Modified Oligonucleotides

    SciTech Connect (OSTI)

    Egli, Martin; Pallan, Pradeep S.; Allerson, Charles R.; Prakash, Thazha P.; Berdeja, Andres; Yu, Jinghua; Lee, Sam; Watt, Andrew; Gaus, Hans; Bhat, Balkrishen; Swayze, Eric E.; Seth, Punit P. (Isis Pharm.); (Vanderbilt)

    2012-03-16T23:59:59.000Z

    The synthesis, biophysical, structural, and biological properties of both isomers of 3'-fluoro hexitol nucleic acid (FHNA and Ara-FHNA) modified oligonucleotides are reported. Synthesis of the FHNA and Ara-FHNA thymine phosphoramidites was efficiently accomplished starting from known sugar precursors. Optimal RNA affinities were observed with a 3'-fluorine atom and nucleobase in a trans-diaxial orientation. The Ara-FHNA analog with an equatorial fluorine was found to be destabilizing. However, the magnitude of destabilization was sequence-dependent. Thus, the loss of stability is sharply reduced when Ara-FHNA residues were inserted at pyrimidine-purine (Py-Pu) steps compared to placement within a stretch of pyrimidines (Py-Py). Crystal structures of A-type DNA duplexes modified with either monomer provide a rationalization for the opposing stability effects and point to a steric origin of the destabilization caused by the Ara-FHNA analog. The sequence dependent effect can be explained by the formation of an internucleotide C-F {hor_ellipsis} H-C pseudo hydrogen bond between F3' of Ara-FHNA and C8-H of the nucleobase from the 3'-adjacent adenosine that is absent at Py-Py steps. In animal experiments, FHNA-modified antisense oligonucleotides formulated in saline showed a potent downregulation of gene expression in liver tissue without producing hepatotoxicity. Our data establish FHNA as a useful modification for antisense therapeutics and also confirm the stabilizing influence of F(Py) {hor_ellipsis} H-C(Pu) pseudo hydrogen bonds in nucleic acid structures.

  15. RegPredict: an integrated system for regulon inference in prokaryotes by comparative genomics approach

    E-Print Network [OSTI]

    Novichkov, Pavel S.

    2010-01-01T23:59:59.000Z

    motifs by comparative genomics. Nucleic Acids Res. , 28,M.S. (2009) Comparative genomics of regulation of fatty acidcomparative and functional genomics. Nucleic Acids Res. ,

  16. acid adenine dinucleotide: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Theoretical Determination of One-Electron Oxidation Potentials for Nucleic Acid Bases Brian T potentials for N-methyl substituted nucleic acid bases guanine, adenine, cytosine,...

  17. Nucleic Acids Research, 1993, Vol. 21, No. 9 2247-2248 The nucleosome repeat length of Kluyveromyces lactis is

    E-Print Network [OSTI]

    for the production of heterologous proteins like prochymosin (7), human serum albumin (8), and human interleukin 1,B each pellet was resuspended in 125 ml TESM. After 10 minutes at room temperature, the cells were spun down, each pellet washed with 225 ml SCE (1 M sorbitol, 0.1 M citric acid, 0.06 M EDTA, pH = 5

  18. Structural Rationalization of a Large Difference in RNA Affinity Despite a Small Difference in Chemistry between Two 2'-O-Modified Nucleic Acid Analogs

    SciTech Connect (OSTI)

    Pattanayek, R.; Sethaphong, L.; Pan, C.; Prhavc, M.; Prakash, T.P.; Manoharan, M.; Egli, M. (Tennessee); (Isis Pharmaceuticals Inc.); (Alnylam Pharmaceuticals,); (Vanderbilt)

    2010-03-08T23:59:59.000Z

    Chemical modification of nucleic acids at the 2'-position of ribose has generated antisense oligonucleotides (AONs) with a range of desirable properties. Electron-withdrawing substituents such as 2'-O-[2-(methoxy)ethyl] (MOE) confer enhanced RNA affinity relative to that of DNA by conformationally preorganizing an AON for pairing with the RNA target and by improving backbone hydration. 2'-Substitution of the ribose has also been shown to increase nuclease resistance and cellular uptake via changes in lipophilicity. Interestingly, incorporation of either 2'-O-[2-(methylamino)-2-oxoethyl]- (NMA) or 2'-O-(N-methylcarbamate)-modified (NMC) residues into AONs has divergent effects on RNA affinity. Incorporation of 2'-O-NMA-T considerably improves RNA affinity while incorporation of 2'-O-NMC-T drastically reduces RNA affinity. Crystal structures at high resolution of A-form DNA duplexes containing either 2'-O-NMA-T or 2'-O-NMC-T shed light on the structural origins of the surprisingly large difference in stability given the relatively minor difference in chemistry between NMA and NMC. NMA substituents adopt an extended conformation and use either their carbonyl oxygen or amino nitrogen to trap water molecules between phosphate group and sugar. The conformational properties of NMA and the observed hydration patterns are reminiscent of those found in the structures of 2'-O-MOE-modified RNA. Conversely, the carbonyl oxygen of NMC and O2 of T are in close contact, providing evidence that an unfavorable electrostatic interaction and the absence of a stable water structure are the main reasons for the loss in thermodynamic stability as a result of incorporation of 2'-O-NMC-modified residues.

  19. Human Retroviruses and AIDS. A compilation and analysis of nucleic acid and amino acid sequences: I--II; III--V

    SciTech Connect (OSTI)

    Myers, G.; Korber, B. [eds.] [Los Alamos National Lab., NM (United States)] [eds.; Los Alamos National Lab., NM (United States); Wain-Hobson, S. [ed.] [Laboratory of Molecular Retrovirology, Pasteur Inst.] [ed.; Laboratory of Molecular Retrovirology, Pasteur Inst.; Smith, R.F. [ed.] [Baylor Coll. of Medicine, Houston, TX (United States). Dept. of Pharmacology] [ed.; Baylor Coll. of Medicine, Houston, TX (United States). Dept. of Pharmacology; Pavlakis, G.N. [ed.] [National Cancer Inst., Frederick, MD (United States). Cancer Research Facility] [ed.; National Cancer Inst., Frederick, MD (United States). Cancer Research Facility

    1993-12-31T23:59:59.000Z

    This compendium and the accompanying floppy diskettes are the result of an effort to compile and rapidly publish all relevant molecular data concerning the human immunodeficiency viruses (HIV) and related retroviruses. The scope of the compendium and database is best summarized by the five parts that it comprises: (I) HIV and SIV Nucleotide Sequences; (II) Amino Acid Sequences; (III) Analyses; (IV) Related Sequences; and (V) Database Communications. Information within all the parts is updated at least twice in each year, which accounts for the modes of binding and pagination in the compendium.

  20. RES Las Vegas

    Broader source: Energy.gov [DOE]

    The National Center for American Indian Enterprise Development is hosting RES Las Vegas including a tradeshow, business expo, procurement, and more on March 9-12, 2015.

  1. Geochemical Rate/RNA Integration Study (GRIST): A Pilot Field Experiment for Inter-Calibration of Biogeochemistry and Nucleic Acid Measurements Final Report

    SciTech Connect (OSTI)

    Bronk, Deborah

    2007-01-08T23:59:59.000Z

    The Geochemical Rate/RNA Integration Study (GRIST) project sought to correlate biogeochemical flux rates with measurements of gene expression and mRNA abundance to demonstrate the application of molecular approaches to estimate the presence and magnitude of a suite of biogeochemical processes. The study was headed by Lee Kerkhoff of Rutgers University. In this component of the GRIST study, we characterized ambient nutrient concentrations and measured uptake rates for dissolved inorganic nitrogen (DIN, ammonium, nitrate and nitrite) and dissolved organic nitrogen (urea and dissolved free amino acids) during two diel studies at the Long-Term Ecosystem Observatory (LEO-15) on the New Jersey continental shelf.

  2. Final Scientific/Technical Report, DE-FG02-06ER64171, Integrated Nucleic Acid System for In-Field Monitoring of Microbial Community Dynamics and Metabolic Activity – Subproject to Co-PI Eric E. Roden

    SciTech Connect (OSTI)

    Eric E. Roden

    2009-07-08T23:59:59.000Z

    This report summarizes research conducted in conjunction with a project entitled “Integrated Nucleic Acid System for In-Field Monitoring of Microbial Community Dynamics and Metabolic Activity”, which was funded through the Integrative Studies Element of the former NABIR Program (now the Environmental Remediation Sciences Program) within the Office of Biological and Environmental Research. Dr. Darrell Chandler (originally at Argonne National Laboratory, now with Akonni Biosystems) was the overall PI/PD for the project. The overall project goals were to (1) apply a model iron-reducer and sulfate-reducer microarray and instrumentation systems to sediment and groundwater samples from the Scheibe et al. FRC Area 2 field site, UMTRA sediments, and other DOE contaminated sites; (2) continue development and expansion of a 16S rRNA/rDNA¬-targeted probe suite for microbial community dynamics as new sequences are obtained from DOE-relevant sites; and (3) address the fundamental molecular biology and analytical chemistry associated with the extraction, purification and analysis of functional genes and mRNA in environmental samples. Work on the UW subproject focused on conducting detailed batch and semicontinuous culture reactor experiments with uranium-contaminated FRC Area 2 sediment. The reactor experiments were designed to provide coherent geochemical and microbiological data in support of microarray analyses of microbial communities in Area 2 sediments undergoing biostimulation with ethanol. A total of four major experiments were conducted (one batch and three semicontinuous culture), three of which (the batch and two semicontinuous culture) provided samples for DNA microarray analysis. A variety of other molecular analyses (clone libraries, 16S PhyloChip, RT-PCR, and T-RFLP) were conducted on parallel samples from the various experiments in order to provide independent information on microbial community response to biostimulation.

  3. Isolated nucleic acids encoding antipathogenic polypeptides and...

    Office of Scientific and Technical Information (OSTI)

    compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes...

  4. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, Oded (Karmey Yosef, IL); Shpiegl, Itai (Rehovot, IL); Goldstein, Marc A. (Davis, CA); Doi, Roy H. (Davis, CA)

    1996-01-01T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  5. Diagnostic method employing MSH2 nucleic acids

    DOE Patents [OSTI]

    Chapelle, A. de la; Vogelstein, B.; Kinzler, K.W.

    1997-12-02T23:59:59.000Z

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error{sup +}(RER{sup +}) tumor cells. 19 figs.

  6. Diagnostic method employing MSH2 nucleic acids

    DOE Patents [OSTI]

    de la Chapelle, Albert (Helsingfors, FI); Vogelstein, Bert (Baltimore, MD); Kinzler, Kenneth W. (Baltimore, MD)

    1997-01-01T23:59:59.000Z

    The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error.sup.+ (RER.sup.+) tumor cells.

  7. Nucleic acids encoding a cellulose binding domain

    DOE Patents [OSTI]

    Shoseyov, O.; Shpiegl, I.; Goldstein, M.A.; Doi, R.H.

    1996-03-05T23:59:59.000Z

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques. 15 figs.

  8. Function and regulation of the Super Elongation Complexes in HIV-1 transcription

    E-Print Network [OSTI]

    Hsu, Joanne H.

    2012-01-01T23:59:59.000Z

    hydroxamic acid SEC Super Elongation Complex TAR Trans-and binding of HEXIM1 to TAR. Nucleic Acids Res 85. Shah M,TAR RNA: critical spacing between the bulge and loop recognition domains. Nucleic Acids

  9. acid biosensor based: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    biosensors of nucleic acids and proteins for point- of-care (POC. The merger of microfluidics and advanced biosensor technolo- gies offers new promises for POC diagnostics...

  10. acids structures applied: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Treatment of software French, James C. 5 Parsing nucleic acid pseudoknotted secondary structure: algorithm and applications Computer Technologies and Information Sciences...

  11. Phytozome: A Comparative Platform for Green Plant Genomics

    E-Print Network [OSTI]

    Goodstein, David M.

    2012-01-01T23:59:59.000Z

    a growing plant comparative genomics resource. Nucleic AcidsL.A. (2011) The Sol Genomics Network (solgenomics.net):for comparative plant genomics. Nucleic Acids Res, 36, D959-

  12. acid transporter specifically: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    nucleic acid concentrations from 1 nM to 5 m Zhang, David Yu 3 Long range transport of acid rain precursors MIT - DSpace Summary: A model of the long range transport of primary...

  13. 128 FloridaEntomologist 91(1) March 2008 EUONITICELLUS INTERMEDIUS (COLEOPTERA:SCARABAEIDAE

    E-Print Network [OSTI]

    Kaufman, Phillip E.

    efficientgenereplacementinthefilamentousfungus Aspergillusnidulans.NucleicAcidsRes.28,E97 43 Brown, J.S. and Holden, D.W. (1998) Insertional

  14. Metagenomics: DNA sequencing of environmental samples

    E-Print Network [OSTI]

    Tringe, Susannah Green; Rubin, Edward M.

    2005-01-01T23:59:59.000Z

    for genome annotation: PRIAM. Nucleic Acids Res 31, 6633-9 (software/gendb/cgi-bin/login.cgi > 81 and PRIAM priam/ > 82 . In general,

  15. ENDANGERED SPECIES RESEARCH Endang Species Res

    E-Print Network [OSTI]

    Pierce, Graham

    May 2009 Published online June 17, 2008 © Inter-Research 2009 · www.int-res.com*Email: rob , Mads C. Forchhammer5 , Charles M. Francis6 , Jennifer A. Gill7 , Brendan J. Godley8 , John Harwood9

  16. ENDANGERED SPECIES RESEARCH Endang Species Res

    E-Print Network [OSTI]

    Hays, Graeme

    . 1980, Houghton et al. 2006, Witt et al. 2007, Godley et al. 2008, this Theme Section [TS]). Yet establishing such © Inter-Research 2008 · www.int-res.com*Email: t.doyle@ucc.ie THEME SECTION Leatherback

  17. Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods

    DOE Patents [OSTI]

    Thompson, David N; Thompson, Vicki S; Schaller, Kastli D; Apel, William A; Reed, David W; Lacey, Jeffrey A

    2013-04-30T23:59:59.000Z

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose, xylobiose, and/or arabinofuranose-substituted xylan using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.

  18. Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods

    DOE Patents [OSTI]

    Thompson, David N. (Idaho Falls, ID); Thompson, Vicki S. (Idaho Falls, ID); Schaller, Kastli D. (Ammon, ID); Apel, William A. (Jackson, WY); Lacey, Jeffrey A. (Idaho Falls, ID); Reed, David W. (Idaho Falls, ID)

    2011-04-12T23:59:59.000Z

    Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose and/or xylobiose using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.

  19. THE INTERACTIONS OF 4-NITROQUINOLINE-1-OXIDE WITH NUCLEIC ACIDS

    E-Print Network [OSTI]

    Winkle, Stephen Alan

    2012-01-01T23:59:59.000Z

    r C2 " C4 CS C6 G2 G4 G5 G6 G8 A6 A8 lS8.06 IS 8.14 IS 6. 43Dimer C2 C4 C5 C6 G2 G4 G5 G6 G8 CpG C T2 T4 T5 T6 A2 A4 A5shift was observed for G8 relative to the other guanine base

  20. Targeting unique nucleic acid structures with small molecules

    E-Print Network [OSTI]

    Tam, Victor Kin-man

    2007-01-01T23:59:59.000Z

    of mono- and disubstitued anthraquinone derivatives with anat opposite ends of the anthraquinone scaffold are employed.

  1. Scanning probe microscopy of nucleic acids and thin organic films

    E-Print Network [OSTI]

    Marat Olegovich Gallyamov

    2011-04-24T23:59:59.000Z

    We developed the models and algorithms to describe two main artefacts of AFM: (i) broadening effect and (ii) decreased heights of profiles for individual objects adsorbed on a hard substrate. It was shown how to measure elastic properties of a single adsorbed microobject. From the viewpoint of contact deformation theory we analysed mechanism of AFM visualisation of an atomic (molecular) structure of a flat surface. We tested technique of immobilisation on a substrate for free single-stranded RNA molecules in an extended state. Using AFM we visualised stages of processes of RNA release from protein coat of tobacco mosaic virus particles. The asymmetry of this process regarding two ends of a macromolecule was confirmed. The dynamics of compaction for DNA T4 molecules was traced using AFM in real time regime. The partially compacted macromolecules were clearly resolved. We detected that the partially compacted structures consisted of toroidal parts formed by different macromolecular strands. The real geometry of the compacted structures was reconstructed on the basis of systematic AFM measurements. That allowed us to calculate the amount of molecules combining each condensed DNA particle. We demonstrated clear benefits of horizontal deposition method for formation of LB films. Using AFM we achieved molecular resolution for some thin film coating and detected lattice parameters with the precision determined by the errors within a few percent. We demonstrated that the structure of the film is determined by the concurrence of several factors: by the closest packing principle for hydrocarbon tails, by the values of surface areas of polar heads at water subphase as well as by the substrate influence.

  2. SAGE-Hindawi Access to Research Journal of Nucleic Acids

    E-Print Network [OSTI]

    Borgstahl, Gloria

    Replication Protein A That Recognize G-Quadruplex DNA Aishwarya Prakash,1 Amarnath Natarajan,1 Luis A. Marky,1

  3. AminoglycosideNucleic Acid Interactions: The Case for Neomycin

    E-Print Network [OSTI]

    Stuart, Steven J.

    . . . . . . . . . . . . . . . . . . . . . . . . . . 156 3.4 Thermodynamics of Drug Binding to the DNA Triplex (ITC) . . . . . . . . . . 157 3.5 CD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161 5.2 The Common Thread that Holds Together RNA Duplex/Triplex, DNA-RNA Hybrid Duplexes, DNA

  4. HiResHiRes Mapping the HighMapping the High Energy UniverseEnergy Universe

    E-Print Network [OSTI]

    HiResHiRes ­­ Mapping the HighMapping the High Energy UniverseEnergy Universe Stefan Westerhoff Columbia University HiRes Collaboration Fermilab Wine & Cheese Seminar 24 October 2003 #12;Particle-rays · Gamma-ray Astronomy ­ photons from MeV to TeV · Cosmic Rays ­ protons and heavier nuclei with energies

  5. ENDANGERED SPECIES RESEARCH Endang Species Res

    E-Print Network [OSTI]

    Exeter, University of

    ' or `marine turtle' and `conserva- © Inter-Research 2010 · www.int-res.com*Corresponding author. Email: b.j.godley , J. Tomás28 , B. P. Wallace29, 30 , T. M. Work31 , J. Zbinden32 , B. J. Godley7,* 1 School of Earth

  6. ENDANGERED SPECIES RESEARCH Endang Species Res

    E-Print Network [OSTI]

    Lewison, Rebecca

    demonstrated marked declines. Reported © Inter-Research 2012 · www.int-res.com *Email: rlewison. Lewison1,*, D. Oro2 , B. J. Godley3 , L. Underhill4 , S. Bearhop3 , R. P. Wilson5 , D. Ainley, J. M. Arcos

  7. ENDANGERED SPECIES RESEARCH Endang Species Res

    E-Print Network [OSTI]

    Hays, Graeme

    -Treviño & Dizon 2000, Duncan et al. 2006), the © Inter-Research 2007 · www.int-res.com*Email: aformia for sampling and diversity Angela Formia1, 4,*, Annette C. Broderick2 , Fiona Glen2, 3 , Brendan J. Godley2

  8. ENDANGERED SPECIES RESEARCH Endang Species Res

    E-Print Network [OSTI]

    Cooke, Steven J.

    threats that face fish residing in large trop- ical rivers such as overfishing, pollution, forestry regionally `vulnerable' in Brazil due to overfishing, pollution, and habitat fragmen- tation. Here, we used Res 15: 103­114, 2011 radio and acoustic telemetry perhaps hold the great- est potential, given

  9. Producing dicarboxylic acids using polyketide synthases

    DOE Patents [OSTI]

    Katz, Leonard; Fortman, Jeffrey L; Keasling, Jay D

    2013-10-29T23:59:59.000Z

    The present invention provides for a polyketide synthase (PKS) capable of synthesizing a dicarboxylic acid (diacid). Such diacids include diketide-diacids and triketide-diacids. The invention includes recombinant nucleic acid encoding the PKS, and host cells comprising the PKS. The invention also includes methods for producing the diacids.

  10. RES North America LLC | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ | Roadmap Jump to:b < RAPID‎RBIRENERCO RenewableRES

  11. LERU Roadmap foR REsEaRch data

    E-Print Network [OSTI]

    Zürich, Universität

    LERU Roadmap foR REsEaRch data LERU REsEaRch data WoRking gRoUp University of Amsterdam, Responsibilities and Skills 28 7 Recommendations 31 #12;3 INTRODUCTION The LERU Roadmap for Research Data that LERU members need to act. In 2011, the LERU community of Chief Information Officers produced a Roadmap

  12. Indian Energy & Energy Infrastructure to be Showcased at RES...

    Energy Savers [EERE]

    to discuss economic development. Indian energy and energy infrastructure are at the heart of our conversations that will be happening at RES. Tracey LeBeau, the Director of our...

  13. #153092 -$15.00 USD Received 14 Sep 2011; revised 14 Oct 2011; accepted 16 Oct 2011; published 1 Nov 2011 (C) 2011 OSA 7 November 2011 / Vol. 19, No. 23 / OPTICS EXPRESS 23333

    E-Print Network [OSTI]

    Cunningham, Brian

    microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles," Nucleic Acids Res. 35, e. MacBeath and S. L. Schreiber, "Printing proteins as microarrays for high-throughput function

  14. Strategies for Enhancing the Effectiveness of Metagenomic-based Enzyme Discovery in Lignocellulolytic Microbial Communities

    E-Print Network [OSTI]

    2010-01-01T23:59:59.000Z

    for genome annotation: PRIAM. Nucleic Acids Res 31:6633–6639November 2008 release of PRIAM [12], modified for nucleotide232,025) at EPRIAM enzyme-specific sequence profiles

  15. Systems biology analysis of Escherichia coli for discovery and metabolic engineering

    E-Print Network [OSTI]

    Orth, Jeffrey David

    2012-01-01T23:59:59.000Z

    for genome annotation: PRIAM. Nucleic Acids Res, 2003. 31(PathoLogic [69], and PRIAM [70]. SimPheny is a commercialdatabase such as MetaCyc. PRIAM is another automated method

  16. higher titer vector, whereas the three births, including the transgenic one, and the blighted

    E-Print Network [OSTI]

    Poggio, Tomaso

    for the successful ho- mologous recombination essential for gene tar- geting (29). The molecular approaches for mak]-labeled probe [Web supplement 5 (41)]. 20. B. Schott et al., Nucleic Acid Res. 25, 2940 (1997). 21. N

  17. Mutant fatty acid desaturase

    DOE Patents [OSTI]

    Shanklin, John; Cahoon, Edgar B.

    2004-02-03T23:59:59.000Z

    The present invention relates to a method for producing mutants of a fatty acid desaturase having a substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons relative to an unmutagenized precursor desaturase having an 18 carbon atom chain length substrate specificity. The method involves inducing one or more mutations in the nucleic acid sequence encoding the precursor desaturase, transforming the mutated sequence into an unsaturated fatty acid auxotroph cell such as MH13 E. coli, culturing the cells in the absence of supplemental unsaturated fatty acids, thereby selecting for recipient cells which have received and which express a mutant fatty acid desaturase with an elevated specificity for fatty acid substrates having chain lengths of less than 18 carbon atoms. A variety of mutants having 16 or fewer carbon atom chain length substrate specificities are produced by this method. Mutant desaturases produced by this method can be introduced via expression vectors into prokaryotic and eukaryotic cells and can also be used in the production of transgenic plants which may be used to produce specific fatty acid products.

  18. Ann Oper Res DOI 10.1007/s10479-012-1077-6

    E-Print Network [OSTI]

    Tesfatsion, Leigh

    Ann Oper Res DOI 10.1007/s10479-012-1077-6 Perspectives of approximate dynamic programming Warren B@princeton.edu #12;Ann Oper Res The wealth of applications has given rise to contributions from different communities

  19. acid amplification test: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    amplification test First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Diagnostic Accuracy of Nucleic Acid...

  20. Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

    DOE Patents [OSTI]

    Jessen, Holly Jean (Chanhassen, MN); Liao, Hans H. (Eden Prairie, MN); Gort, Steven John (Apple Valley, MN); Selifonova, Olga V. (Plymouth, MN)

    2011-10-04T23:59:59.000Z

    The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

  1. Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

    DOE Patents [OSTI]

    Jessen, Holly Jean; Liao, Hans H; Gort, Steven John; Selifonova, Olga V

    2014-11-18T23:59:59.000Z

    The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

  2. RES with RIB F. de Oliveira Santos,a

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    RES with RIB F. de Oliveira Santos,a on behalf of E561S and E521S collaborations GANIL, CEA with Radioactive Ions Beams (RIB) in order to study important unbound states. Three examples of studies are given then decays towards the elastic scattering channel. This fusion reaction occurs for two reasons, firstly

  3. Nano Res (2010) 3: 452458452 Aqueous Supercapacitors on Conductive Cotton

    E-Print Network [OSTI]

    Cui, Yi

    Nano Res (2010) 3: 452­458452 Aqueous Supercapacitors on Conductive Cotton Mauro Pasta1,2 , Fabio the fabrication of wearable supercapacitors using cotton fabric as an essential component. Carbon nanotubes in the supercapacitor. Aqueous lithium sulfate is used as the electrolyte in the devices, because it presents no safety

  4. Property:Incentive/ResCode | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag Jump to: navigation,ProjectStartDate JumpAuth3Link JumpWebsiteExpireDtResCode Jump

  5. Compiling RES Legislation for Kazakhstan | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof EnergyInnovationin UrbanCityCoatedCommunityCompacTecMenlo AvenueCompiling RES

  6. Renewable Energy Systems (RES UK and Ireland) | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ | RoadmapRenewable Energy RFPs HomeResources,(RES UK and

  7. Renewable Energy Systems Inc (RES Americas) | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ | RoadmapRenewable Energy RFPs HomeResources,(RES

  8. Renewable Energy Systems Ltd RES Group | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ | RoadmapRenewable Energy RFPs HomeResources,(RESLtd (RES

  9. Nano Res (2010) 3: 170173170 Synthesis and Characterization of WS2 Inorganic Nanotubes with

    E-Print Network [OSTI]

    Davis, Ben G.

    2010-01-01T23:59:59.000Z

    Nano Res (2010) 3: 170­173170 Synthesis and Characterization of WS2 Inorganic Nanotubes]. Folding and bonding of edge atoms on the periphery of the quasi two-dimensional planar nano- structure this nanotubular structure is suitable for capillary filling using molten metal halides. Nano Res (2010) 3: 170

  10. Nano Res 1 Construction of two-dimensional hydrogen clusters on

    E-Print Network [OSTI]

    Gao, Hongjun

    Nano Res 1 Construction of two-dimensional hydrogen clusters on Au(111) directed by phthalocyanine molecules Kai Yang, Wende Xiao, Liwei Liu, Xiangmin Fei, Hui Chen, Shixuan Du and Hong-Jun Gao () Nano Res. Nano Research DOI 10.1007/s1227401303734 #12;1 TABLE OF CONTENTS (TOC) Construction of Two

  11. 22-10-071RES2020 Implementation of Wind power in

    E-Print Network [OSTI]

    22-10-071RES2020 Implementation of Wind power in RES2020-TIMES Poul Erik Grohnheit Systems Analysis costs ­ cost increase from wind · Elastic demand ­ consumer response to spot prices (cost of demand power: Use of time slices Analysis of hourly electricity demand and wind production for Denmark (east

  12. volume 17 Number 12 1989 Nucleic Acids Research The 5S RNA gene minichromosome of Euplotes

    E-Print Network [OSTI]

    Olins, Ada L.

    extract and 1.33 g/L anhydrous sodium acetate. Algae were harvested and resuspended in Pringsheim solution boiled wheat seeds in Carolina Spring-water. Large-scale cultures of Euplotes were grown in trays

  13. Type I Interferon is Not Just for Viruses: Cytosolic Sensing of Bacterial Nucleic Acids

    E-Print Network [OSTI]

    Monroe, Kathryn McGee

    2011-01-01T23:59:59.000Z

    in the recognition of Helicobacter pylori. Gastroenterologypneumophila, Helicobacter pylori, Francisella tularensis,the focus of Chapter 3. Helicobacter pylori is another gram-

  14. Relations between intraruminal protein and nucleic acid synthesis and forms of urinary nitrogen excreted by sheep

    E-Print Network [OSTI]

    Razzaque, Md. Abdur

    1967-01-01T23:59:59.000Z

    alcohol. The samples were coils. cted in duplicates. An attempt was made to determine the volume of the rumen digesta and its passage out of the reticulo-rumen by usi. ng polyethylene glycol. An appropriate amount of polyethylene glycol (2 gm... procedure for poly- ethylene glycol it was not possible to determine the volume of the rumen digesta and rate of passage out of the reticulo-rumen. Fasting Animals At the end of all experimer. . tal feeding and collection periods, the rumens of two...

  15. DOI: 10.1002/cbic.200600435 Duplex Formation of the Simplified Nucleic Acid

    E-Print Network [OSTI]

    Meggers, Eric

    ) with a stripped-down acyclic backbone can form stable duplexes.[8,9] The propylene glycol nucleotide building sequence and the painstaking separation of anomeric mixtures, this is not the case for GNA. Propylene (GNA) has an acyclic backbone of propylene glycol nucleosides that are connected by phosphodiester

  16. Stable nitrogen isotope measurements of marine bacterial proteins and nucleic acids: tracers of microbial activity

    E-Print Network [OSTI]

    Kovacs, Jeffrey Paul

    1996-01-01T23:59:59.000Z

    Stable nitrogen isotopes ([]15N) can trace elemental cycling in aquatic ecosystems if the enzyme mediated fractionations associated with nutrient uptake and assimilation are negligible or consistently predictable. In this study, bacterial proteins...

  17. Nucleic acids encoding phloem small RNA-binding proteins and transgenic plants comprising them

    DOE Patents [OSTI]

    Lucas, William J.; Yoo, Byung-Chun; Lough, Tony J.; Varkonyi-Gasic, Erika

    2007-03-13T23:59:59.000Z

    The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking, Cucurbita maxima phloem small RNA-binding protein (CmPSRB 1), and the corresponding polypeptide sequence. The invention also provides genetic constructs and transgenic plants comprising the polynucleotide sequence encoding a phloem small RNA-binding protein to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in the plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, systemic resistance to pathogens, responses to environmental stresses, e.g., heat, drought, salinity, and systemic gene silencing (e.g., viral infections).

  18. Cellulases, nucleic acids encoding them and methods for making and using them

    DOE Patents [OSTI]

    Blum, David; Gemsch Cuenca, Joslin; Dycaico, Mark

    2013-04-23T23:59:59.000Z

    This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or .beta.-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or .beta.-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts.

  19. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

    DOE Patents [OSTI]

    Gray, Kevin A. (San Diego, CA); Zhao, Lishan (Emeryville, CA); Cayouette, Michelle H. (San Diego, CA)

    2012-01-24T23:59:59.000Z

    The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

  20. Identification and analysis of hepatitis C virus NS3 helicase inhibitors using nucleic acid binding assays

    E-Print Network [OSTI]

    Mukherjee, Sourav; Hanson, Alica M.; Shadrick, William R.; Ndjomou, Jean; Sweeney, Noreena L.; Hernadez, John J.; Bartczak, Diana; Li, Kelin; Frankowski, Kevin J.; Heck, Julie A.; Arnold, Leggy A.; Schoenen, Frank; Frick, David N,

    2012-06-27T23:59:59.000Z

    Typical assays used to discover and analyze small molecules that inhibit the hepatitis C virus (HCV) NS3 helicase yield few hits and are often confounded by compound interference. Oligonucleotide binding assays are examined ...

  1. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S; Cabantous, Stephanie

    2014-04-01T23:59:59.000Z

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  2. Production of extracellular nucleic acids by genetically altered bacteria in aquatic-environment microcosms

    SciTech Connect (OSTI)

    Paul, J.H.; David, A.W.

    1989-01-01T23:59:59.000Z

    Factors which affect the production of extracellular DNA by genetically altered strains of Escherichia coli, Pseudomonas aeruginosa, Pseudomonas cepacia, and Bradyrhizobium japonicum in aquatic environments were investigated. The presence or absence of the ambient microbial community had little effect on the production of extracellular DNA. Results indicate the extracellular-DNA production by genetically altered bacteria released into aquatic environments is more strongly influenced by physiochemical factors than biotic factors; extracellular-DNA production rates are usually greater for organisms released in freshwater than marine environments; and ambient microbial populations can readily utilize materials released by these organisms.

  3. Nucleic acid encoding a self-assembling split-fluorescent protein system

    DOE Patents [OSTI]

    Waldo, Geoffrey S. (Santa Fe, NM); Cabantous, Stephanie (Los Alamos, NM)

    2011-06-07T23:59:59.000Z

    The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

  4. Response of DNA repair and replication systems to exocyclic nucleic acid base damage

    E-Print Network [OSTI]

    ?r?v?stava, Nidhi

    2012-01-01T23:59:59.000Z

    Genomes experience an often hostile environment that creates a vast array of damages that can give rise to myriad biological outcomes. Fortunately, cells are equipped with networks such as direct reversal, base excision ...

  5. Nucleic Acids Research, 2008, 19 doi:10.1093/nar/gkn836

    E-Print Network [OSTI]

    Dekker, Nynke

    and 2 Institute of Biotechnology and Department of Biological and Environmental Sciences, Viikki active mode. INTRODUCTION Template-directed polymerization of nucleotides (NTPs) is an essential process in all living entities. Accordingly, enzymes catalyzing these processes operate in both cel- lular

  6. Progress Report No. AT(11-1)-3083-6 RE: Nucleic Acids and Protein...

    Office of Scientific and Technical Information (OSTI)

    Chiu, N., Chiu, A. & Suyama, Y. (1974). J. Mol. Biol. (In the ress). 4. Elder, K.T. & Smith, A.E. (1973). Proc. Natl. Acad. Sci. U.S.A . 70, .. 1 2823-2826. ' 5. Gillespie, D. &...

  7. BGL7 beta-glucosidase and nucleic acids encoding the same

    DOE Patents [OSTI]

    Dunn-Coleman, Nigel (Los Gatos, CA); Ward, Michael (San Francisco, CA)

    2012-03-13T23:59:59.000Z

    The present invention provides BGL7 polypeptides with the biological activity of a .beta.-glucosidase and a method of producing a recombinant enzyme having .beta.-glucosidase activity.

  8. Xylanases, nucleic acids encoding them and methods for making and using them

    DOE Patents [OSTI]

    Gray, Kevin A; Dirmeier, Reinhard

    2013-07-16T23:59:59.000Z

    The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal .beta.-1,4-xylosidic linkages or endo-.beta.-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

  9. Reducible Poly(amido ethylenimine)s for Nucleic Acid Delivery

    E-Print Network [OSTI]

    Christensen, Lane

    2006-10-26T23:59:59.000Z

    is reduced 1 2 4 8 16 32 64 -50 -25 0 25 50 EDA/CBA DETA/CBA TETA/CBA n = 5 ? SEM w/DTT w/w Z e t a P o t e n t i a l ( m V ) Bioconjugate Chemistry (2006) 17; 1233-1240. 1 2 3 4 5 6 7 8 9 10 11 12 13 14... in fluorescence #1; Due to reducible disulfide bonds? 12 0 50 250 500 1.0?10 07 1.0?10 08 1.0?10 09 1.0?10 10 SS-PAED bPEI mM BSO R L U / m g P r o t e i n Effect on the Presence of GSH Inhibitor DL -Buthionine Sulfoxamine (BSO) ? BSO decreases intracellular GSH...

  10. Nucleic Acids Research, 2014 1 doi: 10.1093/nar/gku645

    E-Print Network [OSTI]

    Mühlemann, Oliver

    -independent interaction between SMG6 and UPF1 is essential for human NMD Pamela Nicholson1 , Christoph Josi1 , Hitomi be degraded by different routes that all require phosphorylated UPF1 (P-UPF1) as a starting point to be recruited to non- sense mRNAs via an interaction with P-UPF1, leading to eventual mRNA degradation

  11. Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation

    DOE Patents [OSTI]

    Castro, Alonso

    2004-06-01T23:59:59.000Z

    A method for rapid and efficient detection of a target DNA or RNA sequence is provided. A primer having a 3'-hydroxyl group at one end and having a sequence of nucleotides sufficiently homologous with an identifying sequence of nucleotides in the target DNA is selected. The primer is hybridized to the identifying sequence of nucleotides on the DNA or RNA sequence and a reporter molecule is synthesized on the target sequence by progressively binding complementary nucleotides to the primer, where the complementary nucleotides include nucleotides labeled with a fluorophore. Fluorescence emitted by fluorophores on single reporter molecules is detected to identify the target DNA or RNA sequence.

  12. Structure-based model for light-harvesting properties of nucleic acid nanostructures

    E-Print Network [OSTI]

    Pan, Keyao

    Programmed self-assembly of DNA enables the rational design of megadalton-scale macromolecular assemblies with sub-nanometer scale precision. These assemblies can be programmed to serve as structural scaffolds for secondary ...

  13. MOLPROBITY: structure validation and all-atom contact analysis for nucleic acids and their complexes

    E-Print Network [OSTI]

    Richardson, David

    is the addition and full optimization of all hydrogen atoms, bothpolar and nonpolar.The results are reported. The all-atom contact analysis (7) featured on the MOLPROBITY site provides a simple but powerful system uses the information from both hydro- gen bonding and all-atom steric compatibility to fully

  14. An unprecedented nucleic acid capture mechanism for excision of DNA damage

    SciTech Connect (OSTI)

    Rubinson, Emily H.; Prakasha Gowda, A.S.; Spratt, Thomas E.; Gold, Barry; Eichmanbrand, Brandt F. (Pitt); (Vanderbilt); (Penn)

    2010-11-18T23:59:59.000Z

    DNA glycosylases that remove alkylated and deaminated purine nucleobases are essential DNA repair enzymes that protect the genome, and at the same time confound cancer alkylation therapy, by excising cytotoxic N3-methyladenine bases formed by DNA-targeting anticancer compounds. The basis for glycosylase specificity towards N3- and N7-alkylpurines is believed to result from intrinsic instability of the modified bases and not from direct enzyme functional group chemistry. Here we present crystal structures of the recently discovered Bacillus cereus AlkD glycosylase in complex with DNAs containing alkylated, mismatched and abasic nucleotides. Unlike other glycosylases, AlkD captures the extrahelical lesion in a solvent-exposed orientation, providing an illustration for how hydrolysis of N3- and N7-alkylated bases may be facilitated by increased lifetime out of the DNA helix. The structures and supporting biochemical analysis of base flipping and catalysis reveal how the HEAT repeats of AlkD distort the DNA backbone to detect non-Watson-Crick base pairs without duplex intercalation.

  15. Thermo-Biolithography: A Technique for Patterning Nucleic Acids and Proteins

    E-Print Network [OSTI]

    Rubloff, Gary W.

    fluorescent protein (using tyrosinase-initiated conjugation). Because gelatin can be applied and removed under

  16. STRUCTURAL EFFECTS ON THE CIRCULAR DICHROISM OF ETHIDIUM-NUCLEIC ACID COMPLEXES

    E-Print Network [OSTI]

    Dahl, Kenneth Steven

    2013-01-01T23:59:59.000Z

    trup et al. (1978) examined dye binding with pdC- dG-dC-dG (2 dC-dG sites), pdC-dC-dG-dG (l dC-dG site), pdG-dG-dC-dC,1 dC-dG Site). For dye plus pdC-dG-dC-dG, both dC-dG sites

  17. Simultaneous purification and fractionation of nucleic acids and proteins from complex

    E-Print Network [OSTI]

    Santiago, Juan G.

    . Santiago1 * 1 Department of Mechanical Engineering, 2 Department of Chemical Engineering, Stanford, Sterling Heights, MI). We captured images using a 1300 × 1030, 12-bit, interline CCD camera (MicroMAX-1300Y

  18. Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems

    DOE Patents [OSTI]

    Regan, John Frederick

    2014-09-09T23:59:59.000Z

    Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument.

  19. Three- and four-body nonadditivities in nucleic acid tetramers: a CCSD(T) study

    SciTech Connect (OSTI)

    Pitonak, Michal; Neogrady, Pavel; Hobza, Pavel

    2009-12-18T23:59:59.000Z

    Three- and four-body nonadditivities in the uracil tetramer (in DNA-like geometry) and the GC step (in crystal geometry) were investigated at various levels of the wave-function theory: HF, MP2, MP3, L-CCD, CCSD and CCSD(T). All of the calculations were performed using the 6-31G**(0.25,0.15) basis set, whereas the HF, MP2 and the MP3 nonadditivities were, for the sake of comparison, also determined with the much larger aug-cc-pVDZ basis set. The HF and MP2 levels do not provide reliable values for many-body terms, making it necessary to go beyond the MP2 level. The benchmark CCSD(T) three- and four-body nonadditivities are reasonably well reproduced at the MP3 level, and almost quantitative agreement is obtained (fortuitously) either on the L-CCD level or as an average of the MP3 and the CCSD results. Reliable values of many-body terms (especially their higher-order correlation contributions) are obtained already when the rather small 6-31G**(0.25,0.15) basis set is used. The four-body term is much smaller when compared to the three-body terms, but it is definitely not negligible, e.g. in the case of the GC step it represents about 16% of all of the three- and four-body terms. While investigating the geometry dependence of many-body terms for the GG step at the MP3/6-31G**(0.25,0.15) level, we found that it is necessary to include at least three-body terms in the determination of optimal geometry parameters.

  20. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    2002-01-01T23:59:59.000Z

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  1. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    1998-01-01T23:59:59.000Z

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  2. Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants

    DOE Patents [OSTI]

    Somerville, Chris (Portola Valley, CA); van de Loo, Frank (Lexington, KY)

    1997-01-01T23:59:59.000Z

    The present invention relates to the identification of nucleic acid sequences and constructs, and methods related thereto, and the use of these sequences and constructs to produce genetically modified plants for the purpose of altering the composition of plant oils, waxes and related compounds.

  3. Mechanistic insights into the roles of P-TEFb and its novel cofactors in tumorigenesis and HIV transcription

    E-Print Network [OSTI]

    He, Nanhai

    2012-01-01T23:59:59.000Z

    and binding of HEXIM1 to TAR. Nucleic Acids Res 35(13):4347-acid trans-acting response element tris(2-carboxyethyl)phosphine Tat-TARsnRNA TAR TCEP TRM ug ul µM UTR WB WCE WT ribonucleic acid

  4. PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2006; 14:275280

    E-Print Network [OSTI]

    PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2006; 14 COMMUNICATION: ACCELERATED PUBLICATION Photovoltaics Energy Payback Times, Greenhouse Gas Emissions and External University, The Netherlands Life cycle assessments and external cost estimates of photovoltaics have been

  5. Regulatory Design for RES-E Support Mechanisms: Learning Curves, Market Structure, and Burden-Sharing

    E-Print Network [OSTI]

    Batlle, Carlos

    Drawing from relevant experiences in power systems around the world, this paper offers a critical review of existing policy support mechanisms for RES-E (renewable energy sources for electricity), with a detailed analysis ...

  6. Clin Cancer Res. Author manuscript Single nucleotide polymorphisms, apoptosis, and the development of

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Clin Cancer Res. Author manuscript Page /1 7 Single nucleotide polymorphisms, apoptosis apoptosis (RILA) and possess in vitro certain single nucleotide polymorphisms (SNPs) located in candidate ; Genotype ; Humans ; Neoplasms ; genetics ; pathology ; radiotherapy ; Polymorphism, Single Nucleotide

  7. Results from the High-Resolution Fly's Eye (HiRes) Experiment

    E-Print Network [OSTI]

    Aspen 4/06/05 1 Results from the High-Resolution Fly's Eye (HiRes) Experiment Charles Jui (HiRes) University of Utah Physics at the End of the Galactic Cosmic Ray Spectrum Aspen Apr 26-30, 2005 #12;Aspen 4 · Anisotropy · Composition · Proton-air cross-section measurement #12;Aspen 4/06/05 3 The High Resolution Fly

  8. J Bone Miner Res . Author manuscript Proteins, dietary acid load, and calcium and risk of postmenopausal

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    of osteoporosis, but experimental and" " epidemiological evidence is mixed. We aimed to determine whether for fracture risk was observed between RNAE and calcium. Conclusions In this Western population of post-menopausal ; Osteoporosis ; drug therapy ; Postmenopause ; Prospective Studies ; Questionnaires ; Risk INTRODUCTION

  9. EPRI/NRC-RES fire human reliability analysis guidelines.

    SciTech Connect (OSTI)

    Lewis, Stuart R. (Electric Power Research Institute, Charlotte, NC); Cooper, Susan E. (U.S. Nuclear Regulatory Commission, Rockville, MD); Najafi, Bijan (SAIC, Campbell, CA); Collins, Erin (SAIC, Campbell, CA); Hannaman, Bill (SAIC, Campbell, CA); Kohlhepp, Kaydee (Scientech, Tukwila, WA); Grobbelaar, Jan (Scientech, Tukwila, WA); Hill, Kendra (U.S. Nuclear Regulatory Commission, Rockville, MD); Hendrickson, Stacey M. Langfitt; Forester, John Alan; Julius, Jeff (Scientech, Tukwila, WA)

    2010-03-01T23:59:59.000Z

    During the 1990s, the Electric Power Research Institute (EPRI) developed methods for fire risk analysis to support its utility members in the preparation of responses to Generic Letter 88-20, Supplement 4, 'Individual Plant Examination - External Events' (IPEEE). This effort produced a Fire Risk Assessment methodology for operations at power that was used by the majority of U.S. nuclear power plants (NPPs) in support of the IPEEE program and several NPPs overseas. Although these methods were acceptable for accomplishing the objectives of the IPEEE, EPRI and the U.S. Nuclear Regulatory Commission (NRC) recognized that they required upgrades to support current requirements for risk-informed, performance-based (RI/PB) applications. In 2001, EPRI and the USNRC's Office of Nuclear Regulatory Research (RES) embarked on a cooperative project to improve the state-of-the-art in fire risk assessment to support a new risk-informed environment in fire protection. This project produced a consensus document, NUREG/CR-6850 (EPRI 1011989), entitled 'Fire PRA Methodology for Nuclear Power Facilities' which addressed fire risk for at power operations. NUREG/CR-6850 developed high level guidance on the process for identification and inclusion of human failure events (HFEs) into the fire PRA (FPRA), and a methodology for assigning quantitative screening values to these HFEs. It outlined the initial considerations of performance shaping factors (PSFs) and related fire effects that may need to be addressed in developing best-estimate human error probabilities (HEPs). However, NUREG/CR-6850 did not describe a methodology to develop best-estimate HEPs given the PSFs and the fire-related effects. In 2007, EPRI and RES embarked on another cooperative project to develop explicit guidance for estimating HEPs for human failure events under fire generated conditions, building upon existing human reliability analysis (HRA) methods. This document provides a methodology and guidance for conducting a fire HRA. This process includes identification and definition of post-fire human failure events, qualitative analysis, quantification, recovery, dependency, and uncertainty. This document provides three approaches to quantification: screening, scoping, and detailed HRA. Screening is based on the guidance in NUREG/CR-6850, with some additional guidance for scenarios with long time windows. Scoping is a new approach to quantification developed specifically to support the iterative nature of fire PRA quantification. Scoping is intended to provide less conservative HEPs than screening, but requires fewer resources than a detailed HRA analysis. For detailed HRA quantification, guidance has been developed on how to apply existing methods to assess post-fire fire HEPs.

  10. Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination

    DOE Patents [OSTI]

    Xie, Jianming (San Diego, CA); Wang, Lei (San Diego, CA); Wu, Ning (Boston, MA); Schultz, Peter G. (La Jolla, CA)

    2008-07-15T23:59:59.000Z

    Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino acids. Methods of determining the structure of a protein, e.g., a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

  11. Anim. Res. 54 (2005) 413424 INRA, EDP Sciences, 2005

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    caponisationonfat composition byparts(wing, breast,thigh,and drumstick) and tissues (skin, subcutaneous adipose.13%inwing;21.09%to49.57% in thigh; 14.33% to 24.82% in drumstick) and led to minor modifications in fat characteristics, par- ticularly in the thigh and the drumstick, where the unsaturated vs. saturated fatty acid

  12. UTILITY_ID","UTILNAME","STATE_CODE","YEAR","MONTH","RES_REV (Thousand $)","RES_S

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are nowTotal" (Percent) Type: Sulfur Content APIMwH)","RES_CONS ","COM_REV (Thousand $)","COM_SALES

  13. UTILITY_ID","UTILNAME","STATE_CODE","YEAR","MONTH","RES_REV (Thousand $)","RES_S

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are nowTotal" (Percent) Type: Sulfur Content APIMwH)","RES_CONS ","COM_REV (Thousand

  14. UTILITY_ID","UTILNAME","STATE_CODE","YEAR","MONTH","RES_REV (Thousand $)","RES_S

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are nowTotal" (Percent) Type: Sulfur Content APIMwH)","RES_CONS ","COM_REV

  15. UTILITY_ID","UTILNAME","STATE_CODE","YEAR","MONTH","RES_REV (Thousand $)","RES_S

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are nowTotal" (Percent) Type: Sulfur Content APIMwH)","RES_CONS

  16. Methods and compounds for chemical ligation

    DOE Patents [OSTI]

    Church, George M.; Sismour, A. Michael

    2013-07-09T23:59:59.000Z

    Compositions and methods for chemical ligation are provided. Methods for nucleic acid sequencing, nucleic acid assembly and nucleic acid synthesis are also provided.

  17. Aust. J. Mar. Freshw. Res., 1984, 35, 119-28 Aromatic Hydrocarbons in Waters of

    E-Print Network [OSTI]

    Canberra, University of

    Aust. J. Mar. Freshw. Res., 1984, 35, 119-28 Aromatic Hydrocarbons in Waters of Port Phillip Bay of aromatic hydrocarbons in coastal waters, using solvent extraction and fluorescence emission analysis, shows and the Yarra River estuary. Introduction Pollution of the marine environment by petroleum hydrocarbons

  18. PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2009; 17:1133

    E-Print Network [OSTI]

    PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2009; 17 Research History of Accelerated and Qualification Testing of Terrestrial Photovoltaic Modules: A Literature-plate terrestrial photovoltaic (PV) modules. An important facet of this subject is the standard module test

  19. PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. (in press)

    E-Print Network [OSTI]

    PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. (in press and Separation of Cd and Te from Cadmium Telluride Photovoltaic Manufacturing Scrap V. M. Fthenakis*,y and W. Wang Brookhaven National Laboratory, Department of Environmental Sciences, Photovoltaic Environmental

  20. PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2006; 14:2543

    E-Print Network [OSTI]

    Anderson, Timothy J.

    PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2006; 14 Solar Energy, Inc., 5575 S. Houghton Rd, Tucson, AZ 85747, USA 7 Energy Photovoltaics, Inc., 276 Bakers transport within copper indium gallium sulfoselenide (CIGSS) photovoltaic devices, whose absorber layers

  1. , J. Geophys. Res., 103, 24469-24486 Stress Coupling Between Earthquakes in Northwest Turkey

    E-Print Network [OSTI]

    Hubert-Ferrari, Aurélia

    , J. Geophys. Res., 103, 24469-24486 Stress Coupling Between Earthquakes in Northwest Turkey, Istanbul, Turkey Aurélia Hubert and Geoffrey C. P. King Laboratoire de Tectonique et Mécanique de la stress interactions of 29 earthquakes (Ms 6.0) that have occurred in the region of northwest Turkey

  2. INTERNATIONAL JOURNAL OF ENERGY RESEARCH Int. J. Energy Res. 2009; 33:481498

    E-Print Network [OSTI]

    Azad, Abdul-Majeed

    , mild process conditions and solar energy as the MSR actuator obviates several drawbacks plaguing 43606-3390, U.S.A. y E-mail: abdul-majeed.azad@utoledo.edu Contract/grant sponsor: Department of EnergyINTERNATIONAL JOURNAL OF ENERGY RESEARCH Int. J. Energy Res. 2009; 33:481­498 Published online 2

  3. Laser Pointer System Intuitive Interaction for Large High-Res Displays

    E-Print Network [OSTI]

    Reiterer, Harald

    Laser Pointer System Intuitive Interaction for Large High-Res Displays LEDs Accelerometer Vibration Buttons Laser & LEDs Unlike conventional input devices like mouse and keyboard, laser pointers do that are displayed on large high-resolution screens. The laser pointer technology was developed in cooperation

  4. Regular PaperJ. Radiat. Res., 51, 657664 (2010) Adaptive Response in Zebrafish Embryos Induced Using

    E-Print Network [OSTI]

    Yu, K.N.

    2010-01-01T23:59:59.000Z

    in the zebrafish embryos in vivo. INTRODUCTION For radiation protection purposes, prediction of risk from this model is commonly adopted for radiation protection purposes, there is a considerable amount of evidenceRegular PaperJ. Radiat. Res., 51, 657­664 (2010) Adaptive Response in Zebrafish Embryos Induced

  5. Nano Res. 2011, 4(3): 290296290 Hybrid Silicon-Carbon Nanostructured Composites as Superior

    E-Print Network [OSTI]

    Zhou, Chongwu

    ­6]. Among the above-mentioned energy conversion and storage devices, lithium ion batteries have been widely as components for next-generation electrochemical energy conversion and storage devices [9­11]. For instance, TiNano Res. 2011, 4(3): 290­296290 Hybrid Silicon-Carbon Nanostructured Composites as Superior Anodes

  6. NLEL-MAAT at CLEF-ResPubliQA Santiago Correa, Davide Buscaldi, Paolo Rosso.

    E-Print Network [OSTI]

    Rosso, Paolo

    NLEL-MAAT at CLEF-ResPubliQA Santiago Correa, Davide Buscaldi, Paolo Rosso. NLE Lab, ELiRF Research Group, DSIC, Universidad Politécnica de Valencia, Spain. {scorrea, dbuscaldi, prosso}@dsic.upv.es http://users.dsic.upv.es/grupos/nle Abstract. This report presents the work carried out at NLE Lab for the QA@CLEF-2009 competition. We used

  7. three Atlantic fishing areas. J. Fish. Res. Board Can. 11:171-197.

    E-Print Network [OSTI]

    Rhode Island Kingston, RI02881 DAVID H. KESLER Division ofBiological Sciences University of Michigan Ann Arbor homeostasis. J. Fish. Res. Board Can. 33:63-75. W. HUNTrING HOWELL Department ofZoology University of.I) with or without an iodophor (Wyandotte Co.) con- tained in ice for controlling microbial outgrowth of a mixture

  8. Diabetes Metab Res Rev. Author manuscript Anti-CD38 autoantibodies in type? diabetes

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Diabetes Metab Res Rev. Author manuscript Page /1 12 Anti-CD38 autoantibodies in type? diabetes.mallone@inserm.fr> Abstract Human diabetes mellitus comprises two main clinical entities: type 1 and type 2 diabetes. While type 1 diabetes is autoimmune in origin, type 2 diabetes is due to a decreased sensitivity to insulin

  9. DIABETES/METABOLISM RESEARCH AND REVIEWS RESEARCH ARTICLE Diabetes Metab Res Rev 2005; 21: 264270.

    E-Print Network [OSTI]

    Crawford, John R.

    DIABETES/METABOLISM RESEARCH AND REVIEWS RESEARCH ARTICLE Diabetes Metab Res Rev 2005; 21: 264 Design and development of a scale measuring fear of complications in type 1 diabetes Emily P. Taylor1 John R. Crawford2 Ann E. Gold3* 1 Department of Diabetes, Grampian University Hospitals Trust, Aberdeen

  10. DIABETES/METABOLISM RESEARCH AND REVIEWS RESEARCH ARTICLE Diabetes Metab Res Rev 2003; 19: 288298.

    E-Print Network [OSTI]

    Sastry, Ann Marie

    DIABETES/METABOLISM RESEARCH AND REVIEWS RESEARCH ARTICLE Diabetes Metab Res Rev 2003; 19: 288 Nerve collagens from diabetic and nondiabetic Sprague­Dawley and biobreeding rats: an atomic force Accepted: 14 January 2003 Abstract Background Alterations in rat's nerve collagens due to diabetes may

  11. DIABETES/METABOLISM RESEARCH AND REVIEWS REVIEW ARTICLE Diabetes Metab Res Rev 2008; 24: 604610.

    E-Print Network [OSTI]

    Strathclyde, University of

    DIABETES/METABOLISM RESEARCH AND REVIEWS REVIEW ARTICLE Diabetes Metab Res Rev 2008; 24: 604 Nanomedicine and its potential in diabetes research and practice John C. Pickup1* Zheng-Liang Zhi1 Faaizah Khan­100 nm. Although the science is still in its infancy, it has major potential applications in diabetes

  12. Amer J of Potato Res (2001) 78:319-322 319 Short Communication

    E-Print Network [OSTI]

    Douches, David S.

    2001-01-01T23:59:59.000Z

    Amer J of Potato Res (2001) 78:319-322 319 Short Communication Susceptibility of Potato (Solanum:kirkw@msu.edu ABSTRACT Late blight is an important disease of potato (Solanum tuberosum L.) worldwide, and therefore, many potato-breeding programs have prioritized the develop- ment of late blight-resistant potato

  13. Amer J of Potato Res (2001) 78:421424 421 Michigan Purple

    E-Print Network [OSTI]

    Douches, David S.

    2001-01-01T23:59:59.000Z

    Amer J of Potato Res (2001) 78:421424 421 Michigan Purple: A Purple-Skinned Tablestock Variety D. S-ovoid shaped, purple- skinnedtablestock potato variety (Solanum tuberosum L.) devel- oped at Michigan State Purple from field trial at Montcalm Potato Research Farm. Accepted for publicationAugust2, 2001

  14. Amer J of Potato Res (2002) 79:451-457 451 Foliar Resistance to Late Blight

    E-Print Network [OSTI]

    Douches, David S.

    2002-01-01T23:59:59.000Z

    Amer J of Potato Res (2002) 79:451-457 451 Foliar Resistance to Late Blight in Potato Clones States and other parts of the world pose a sig- nificant threat to potato production. A continual evalua- tion of potato clones for resistance to late blight is necessary to identify clones with resistance

  15. Biochemistry 1989, 28, 2873-2881 2873 Ponticelli, A. S.,Schultz, D. W., Taylor, A. F., & Smith, G.

    E-Print Network [OSTI]

    Kowalczykowski, Stephen C.

    Biochemistry 1989, 28, 2873-2881 2873 Ponticelli, A. S.,Schultz, D. W., Taylor, A. F., & Smith, G. M., Schultz, D. W., & Taylor, A. F. (1984) Cold Spring Harbor Symp. Quant. Biol. 49, 485-495. Stahl, F. W. (1986) Prog. Nucleic Acid Res. Mol. Biol. 33, R. (1985) Cell (Cambridge, Mass.) 41, 145

  16. US defensive operations against Libya and the nuclear accident at Chernobyl. Markup before the Committee on Foreign Affairs, House of Representatives, Ninety-Ninth Congress, Second Session on H. Res. 424 and H. Res 440, May 1, 1986

    SciTech Connect (OSTI)

    Not Available

    1986-01-01T23:59:59.000Z

    The House Foreign Affairs Committee met to mark up two resolutions: H. Res. 424 and H. Res. 440. H. Res. 424 thanks the United Kingdom for its assistance in the April 14, 1986 operation against Libya. Despite objections to the raid and to including the British, as well as questions about the quality of the US response and about the President's compliance with the Constitution and the War Powers Resolution, the resolution passed. H. Res. 440 expresses sympathy to the victims of the Chernobyl accident and asks the Soviet Union to relax restrictions on communications and the transfer of whatever technology and assistance will be helpful. It also criticizes the Soviet handling of information about the accident. An amendment strengthened the wording of the criticism, and the resolution passed. The report includes the committee discussion and the tests of the two resolutions.

  17. UTILITY_ID","UTILNAME","STATE_CODE","YEAR","MONTH","RES_REV (Thousand $)","RES_S

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are nowTotal" (Percent) Type: Sulfur Content APIMwH)","RES_CONS ","COM_REV (Thousand $)","COM_SALES (MwH)","COM_CONS","IND_REV

  18. 2011;71:245-254. Published online January 2, 2011.Cancer Res Esmeralda Casas, Jihoon Kim, Andrs Bendesky, et al.

    E-Print Network [OSTI]

    Wolfe, Cecily J.

    2011;71:245-254. Published online January 2, 2011.Cancer Res Esmeralda Casas, Jihoon Kim, Andrés of Twist1-Induced Epithelial Mesenchymal Transition and Metastasis Esmeralda Casas1,2 , Jihoon Kim3

  19. Thermodynamics and Enzymatic Polymerization of Artificial Metallo-Nucleic Acids AND Investigation of Duplex Formation between GAN and RNA

    E-Print Network [OSTI]

    Kim, Eun Kyong

    2011-01-01T23:59:59.000Z

    cells on a Varian Cary 500 UV-Vis spectrophotometer equipped with a Peltiercell on a Varian Cary 500 UV-Vis spectrophotometer equipped with a Peltier

  20. Electrical Detection of Nucleic Acid Amplification Using an On-Chip Quasi-Reference Electrode and a PVC REFET

    E-Print Network [OSTI]

    Bashir, Rashid

    and a PVC REFET Eric Salm,, Yu Zhong,,§ Bobby Reddy, Jr.,,§ Carlos Duarte-Guevara,,§ Vikhram Swaminathan that utilizes a platinum QRE to establish a pH-sensitive fluid gate potential and a PVC membrane REFET to enable

  1. Calibration and Testing of a Water Model for Simulation of the Molecular Dynamics of Proteins and Nucleic Acids in Solution

    E-Print Network [OSTI]

    Levitt, Michael

    Calibration and Testing of a Water Model for Simulation of the Molecular Dynamics of Proteins important in biological macromolecules, where fewer experimental results are available for calibration. Our

  2. Electronic structure and spectroscopy of nucleic acid bases: Ionization energies, ionization-induced structural changes, and photoelectron spectra

    SciTech Connect (OSTI)

    Bravaya, Ksenia B.; Kostko, Oleg; Dolgikh, Stanislav; Landau, Arie; Ahmed, Musahid; Krylov, Anna I.

    2010-08-02T23:59:59.000Z

    We report high-level ab initio calculations and single-photon ionization mass spectrometry study of ionization of adenine (A), thymine (T), cytosine (C) and guanine (G). For thymine and adenine, only the lowest-energy tautomers were considered, whereas for cytosine and guanine we characterized five lowest-energy tautomeric forms. The first adiabatic and several vertical ionization energies were computed using equation-of-motion coupled-cluster method for ionization potentials with single and double substitutions. Equilibrium structures of the cationic ground states were characterized by DFT with the {omega}B97X-D functional. The ionization-induced geometry changes of the bases are consistent with the shapes of the corresponding molecular orbitals. For the lowest-energy tautomers, the magnitude of the structural relaxation decreases in the following series G > C > A > T, the respective relaxation energies being 0.41, 0.32, 0.25 and 0.20 eV. The computed adiabatic ionization energies (8.13, 8.89, 8.51-8.67 and 7.75-7.87 eV for A,T,C and G, respectively) agree well with the onsets of the photoionization efficiency (PIE) curves (8.20 {+-} 0.05, 8.95 {+-} 0.05, 8.60 {+-} 0.05 and 7.75 {+-} 0.05 eV). Vibrational progressions for the S{sub 0}-D{sub 0} vibronic bands computed within double-harmonic approximation with Duschinsky rotations are compared with previously reported experimental photoelectron spectra.

  3. Discrimination of Alternative Spliced Isoforms by Real-Time PCR Using Locked Nucleic Acid (LNA) Substituted Primer

    E-Print Network [OSTI]

    Wan, Guoqiang

    Determination of quantitative expression levels of alternatively spliced isoforms provides an important approach to the understanding of the functional significance of each isoform. Real-time PCR using exon junction ...

  4. 1998 Oxford University Press42804290 Nucleic Acids Research, 1998, Vol. 26, No. 18 A database of macromolecular motions

    E-Print Network [OSTI]

    Gerstein, Mark

    to implement the database. However, the complexity and heterogeneity of the information kept in the database, are involved in many basic functions such as catalysis, regulation of activity, transport of metabolites

  5. 2001 Oxford University Press Nucleic Acids Research, 2001, Vol. 29, No. 22 46634673 Importance of the conserved nucleotides around the

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer- messenger RNA for protein nucleotide stretches 16­20 and 333­335 seriously impair protein tagging with only minor changes nucleotides next to the tRNA-like portion is proposed. Overall, the highly conserved nucleotides around the t

  6. Nucleic Acids Research, 1994, Vol. 22, No. 11 1981 -1987 Interaction between the first and last nucleotides of

    E-Print Network [OSTI]

    Chanfreau, Guillaume

    nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S.cerevisiae Guillaume be reactivated by a G to C substitution of the last intron nucleotide. These results demonstrate that the inter- action between the first and last intron nucleotides is a conserved feature of nuclear pre-mRNA splicing

  7. Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same

    DOE Patents [OSTI]

    Chee, Mark (Palo Alto, CA); Gingeras, Thomas R. (Santa Clara, CA); Fodor, Stephen P. A. (Palo Alto, CA); Hubble, Earl A. (Mountain View, CA); Morris, MacDonald S. (San Jose, CA)

    1999-01-19T23:59:59.000Z

    The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.

  8. Volume 15 Number 19 1987 Nucleic Acids Research Deduced prducts of C4-dkarboxylate tranort regultory genes of Rukobium kgminowsaum

    E-Print Network [OSTI]

    Ausubel, Frederick M.

    of the dctD gene poduct (DctD)was strongly conserved with N-terminal domains of theproducts of several

  9. Activation of nucleic acid-sensing Toll-like receptors requires cleavage by endolysosomal proteases: a mechanism to avoid autoimmunity

    E-Print Network [OSTI]

    Ewald, Sarah Elisabeth

    2010-01-01T23:59:59.000Z

    receptor DC: dendritic cell pDC: plasmacytoid dendritic celltaken up by plasmacytoid DCs (pDC) and elicit large amountsare also components of the pDC-MyD88 signaling complex (

  10. Volume 4 Number 6 June 1977 Nucleic Acids Research Conformational states of chromatin v bodies induced by urea

    E-Print Network [OSTI]

    Olins, Ada L.

    and 7 M urea. Companion studies on the conformation of the inner histone "heterotypic tetramer" also pairs. The inner histone "heterotypic tetramer" (one each of H4, H3, H2A, and H2B, devoid of DNA/ml solution A^o =3.5 (12). The heterotypic tetramer was fractionated from HI and H5 by layering 0.5 ml

  11. EPRI/NRC-RES fire PRA guide for nuclear power facilities. Volume 1, summary and overview.

    SciTech Connect (OSTI)

    Not Available

    2004-09-01T23:59:59.000Z

    This report documents state-of-the-art methods, tools, and data for the conduct of a fire Probabilistic Risk Assessment (PRA) for a commercial nuclear power plant (NPP) application. The methods have been developed under the Fire Risk Re-quantification Study. This study was conducted as a joint activity between EPRI and the U. S. NRC Office of Nuclear Regulatory Research (RES) under the terms of an EPRI/RES Memorandum of Understanding [RS.1] and an accompanying Fire Research Addendum [RS.2]. Industry participants supported demonstration analyses and provided peer review of this methodology. The documented methods are intended to support future applications of Fire PRA, including risk-informed regulatory applications. The documented method reflects state-of-the-art fire risk analysis approaches. The primary objective of the Fire Risk Study was to consolidate recent research and development activities into a single state-of-the-art fire PRA analysis methodology. Methodological issues raised in past fire risk analyses, including the Individual Plant Examination of External Events (IPEEE) fire analyses, have been addressed to the extent allowed by the current state-of-the-art and the overall project scope. Methodological debates were resolved through a consensus process between experts representing both EPRI and RES. The consensus process included a provision whereby each major party (EPRI and RES) could maintain differing technical positions if consensus could not be reached. No cases were encountered where this provision was invoked. While the primary objective of the project was to consolidate existing state-of-the-art methods, in many areas, the newly documented methods represent a significant advancement over previously documented methods. In several areas, this project has, in fact, developed new methods and approaches. Such advances typically relate to areas of past methodological debate.

  12. NLEL-MAAT at ResPubliQA Santiago Correa and Davide Buscaldi and Paolo Rosso

    E-Print Network [OSTI]

    Rosso, Paolo

    NLEL-MAAT at ResPubliQA Santiago Correa and Davide Buscaldi and Paolo Rosso NLE Lab, ELiRF Research Group, DSIC, Universidad Polit´ecnica de Valencia, Spain. {scorrea, dbuscaldi, prosso}@dsic.upv.es http://users.dsic.upv.es/grupos/nle Abstract. This report presents the work carried out at NLE Lab for the QA@CLEF-2009 competition. We used

  13. Influence of H2S and Thiols on the Binding of Alkenes and Alkynes to ReS4 Spectator Sulfido Effect

    E-Print Network [OSTI]

    Rauchfuss, Thomas B.

    Influence of H2S and Thiols on the Binding of Alkenes and Alkynes to ReS4 -: The Spectator Sulfido-component reaction of ReS4 - (1), H2S, and unsaturated substrates (un ) alkene, alkyne) affords the ReV derivatives Re(S)(S2un)(SH)2 -. These adducts arise via the addition of H2S to intermediate dithiolates ReS2(S2C2

  14. int. j. remote sensing, 2000, vol. 21, no. 16, 30713082 Satellite-based mapping of Canadian boreal forest res: evaluation and

    E-Print Network [OSTI]

    Li, Zhanqing

    forest res: evaluation and comparison of algorithms Z. LI*, S. NADON, J. CIHLAR and B. STOCKS§ Canada for the underestimation.While this problem is alleviated by taking advantage of NDVI changes, a simple combination burned scars created by res taking place in previous years. A step-by-step comparison between the two

  15. Nano Res. 2012, 5(6): 412420412 Reshaping the Tips of ZnO Nanowires by Pulsed Laser

    E-Print Network [OSTI]

    Wang, Zhong L.

    Nano Res. 2012, 5(6): 412­420412 Reshaping the Tips of ZnO Nanowires by Pulsed Laser Irradiation to the body of the ZnO nanowire, and that the center of the sphere is hollow. The growth mechanism of the hollow ZnO nanospheres is proposed to involve laser-induced ZnO evaporation immediately followed by re

  16. PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2004; 12:93111 (DOI: 10.1002/pip.527)

    E-Print Network [OSTI]

    Romeo, Alessandro

    PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2004; 12(In,Ga)Se2; thin-films; photovoltaics; solar energy INTRODUCTION P olycrystalline thin-film solar cells the complete solar spectrum for photovoltaic power conversion. There are several chalcopyr

  17. PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2004; 12:3338 (DOI: 10.1002/pip.525)

    E-Print Network [OSTI]

    Romeo, Alessandro

    PROGRESS IN PHOTOVOLTAICS: RESEARCH AND APPLICATIONS Prog. Photovolt: Res. Appl. 2004; 12 INTRODUCTION T he polycrystalline CdTe/CdS thin-film solar cell is one of the most important photovoltaic,2 Recent measure- ments of the photovoltaic performance of CdTe solar cells irradiated with high

  18. Ind. Eng. Chem. Res. 1996,34, 4395-4405 4396 Multiple Steady States and Instability in Distillation. Implications

    E-Print Network [OSTI]

    Skogestad, Sigurd

    Ind. Eng. Chem. Res. 1996,34, 4395-4405 4396 Multiple Steady States and Instability in Distillation-7034 Trondheim, Norway The fact that distillation columns, even in the ideal binary case, may display addresses some implications of these phenomena for the operation and control of distillation columns. Under

  19. [21] M.I. Reiman. Open queueing networks in heavy traffic. Math. of Oper. Res. , 9:441--458, 1984.

    E-Print Network [OSTI]

    .D. thesis, Ludwig­Maximilian­Universit¨at M¨unchen, 1996. [16] A. Mandelbaum. The dynamic complementarity traffic limit theorem for networks of queues with multiple customer types. Math. of Oper. Res., 16 are equal to 1. The determinant of the matrix itself is 1 \\Gamma ` 1 ` 2 ` 3 ` 4 . Now consider D k 3 . Once

  20. Short CommunicationJ. Radiat. Res., 53, 475481 (2012) Triphasic Low-dose Response in Zebrafish Embryos Irradiated by

    E-Print Network [OSTI]

    Yu, K.N.

    2012-01-01T23:59:59.000Z

    the linear no-threshold (LNT) hypothesis for radiation protection considerations, which assumes that the riskShort CommunicationJ. Radiat. Res., 53, 475­481 (2012) Triphasic Low-dose Response in Zebrafish, the experimental data were in line with induc- tion of radiation-induced bystander effect as well as rescue effect

  1. 10.1101/gr.3727505Access the most recent version at doi: 2005 15: 1611-1619Genome Res.

    E-Print Network [OSTI]

    Botstein, David

    10.1101/gr.3727505Access the most recent version at doi: 2005 15: 1611-1619Genome Res. Kara Dolinski and David Botstein genome sequence Changing perspectives in yeast research nearly a decade after the References http://genome.cshlp.org/content/15/12/1611.full.html#related-urls Article cited in: http://genome

  2. Introduction to Weather and Climate Lab L1 Monday, 2:00-4:40 pm Nat. Res. 108

    E-Print Network [OSTI]

    ATS 351 Introduction to Weather and Climate Lab Fall 2013 L1 ­ Monday, 2:00-4:40 pm ­ Nat. Res. 108, Lab 10 returned 15*+ 12/9 Severe Weather and Lightning 13 Lab 12 due, Lab 11 returned 16 Climate

  3. Ann Oper Res (2011) 187:4563 DOI 10.1007/s10479-009-0643-z

    E-Print Network [OSTI]

    Hochbaum, Dorit S.

    2011-01-01T23:59:59.000Z

    Ann Oper Res (2011) 187:45­63 DOI 10.1007/s10479-009-0643-z Nuclear threat detection with mobile capabilities. Keywords Nuclear threat detection · Network flow · Parametric cut · Counter-terrorism · Discrete in such a situation. Detecting nuclear threats is a challenging problem under any circumstances. Distrib- uted Sensor

  4. Amer J of Potato Res (2001) 78:159-165 159 Evaluation of Brfld Solanum Species for Resistance

    E-Print Network [OSTI]

    Douches, David S.

    2001-01-01T23:59:59.000Z

    Amer J of Potato Res (2001) 78:159-165 159 Evaluation of Brfld Solanum Species for Resistance potato x wild species (diploid or tetraploid) from Mexico, Russia, or South America was inoculated accessions (176/546) were retested, and 56 genotypes were identified as having potential for use in potato

  5. Amer J of Potato Res (2005) 82:139-146 139 Effect of Temperature and Photoperiod on Symptoms Associated

    E-Print Network [OSTI]

    Douches, David S.

    2005-01-01T23:59:59.000Z

    Amer J of Potato Res (2005) 82:139-146 139 Effect of Temperature and Photoperiod on Symptoms Potato Cultivars O. A. Rubio-Covarrubias 1,~, D. S. Douches 3, R. Hammerschmidt 2, A. daRocha 2, and W. W infes- tans in five potato cultivars with and without resistance (R) genes was investigated. Four

  6. Smart Grid Demos Provide Guidance on Integrating DER and RES into the Distribution System with Consideration of Transmission Impacts, Market Signals, and Technologies

    SciTech Connect (OSTI)

    Kueck, John D [ORNL] [ORNL; Hamilton, Stephanie [Electric Power Research Institute (EPRI)] [Electric Power Research Institute (EPRI); Smith, Merrill [U.S. Department of Energy] [U.S. Department of Energy

    2010-01-01T23:59:59.000Z

    This paper describes the overall process for developing a planning criteria and deployment strategy for technology applications under the US Department of Energy (USDOE) and Electric Power Research Institute (EPRI) Smart Grid programs. These activities described provide an understanding of each demonstration and how they individually and as group further industry knowledge of Distributed Energy Resources (DER) and Renewable Energy Sources (RES) impact the grid and how the distribution grid can interact with DER and RES in smart ways. Both USDOE through its Renewable and Distributed Systems Integration (RDSI) and EPRI via its Smart Grid Demonstration Program both assess how DER and RES can be integrated and operated to lower the carbon footprint.

  7. Technical Paper ISSN 1997-1400 Int. J. Pavement Res. Technol. 3(5):234-240 Copyright @ Chinese Society of Pavement Engineering

    E-Print Network [OSTI]

    Bentz, Dale P.

    Technical Paper ISSN 1997-1400 Int. J. Pavement Res. Technol. 3(5):234-240 Copyright @ Chinese Society of Pavement Engineering Vol.3 No.5 Sep. 2010 International Journal of Pavement Research

  8. 10.1101/gr.3866105Access the most recent version at doi: 2005 15: 1777-1786Genome Res.

    E-Print Network [OSTI]

    Cheng, Jianlin Jack

    10.1101/gr.3866105Access the most recent version at doi: 2005 15: 1777-1786Genome Res. Michael R. Brent ORF at each locus Genome annotation past, present, and future: How to define an References http://genome.cshlp.org/content/15/12/1777.full.html#related-urls Article cited in: http://genome.cshlp.org/content/15/12/1777.full

  9. INTERNATIONAL JOURNAL OF ENERGY RESEARCH Int. J. Energy Res. 2010; 34:152163

    E-Print Network [OSTI]

    ; for example, the model by Tenno et al. for a lead acid battery [2] is valid only under overcharge. Verbrugge of the SOC for lead acid and nickel­cadmium cells. There are several limitations associated with each method power lithium ion batteries are increasingly used in power tools, hybrid electric vehicles and military

  10. Prolactin messenger ribonucleic acid concentrations throughout the ovine estrous cycle: Assessment relative to prolactin serum and pituitary amounts

    SciTech Connect (OSTI)

    Landefeld, T.; Roulia, V.; Bagnell, T.; Ballard, T.; Levitan, I. (Univ. of Michigan, Ann Arbor (USA))

    1991-01-01T23:59:59.000Z

    Prolactin (PRL) mRNA concentrations were assessed by nucleic acid hybridization assays in pituitaries of ewes representing the defined stages of the ovine estrous cycle. Concomitantly, pituitary and serum PRL concentrations were measured in these ewes using radioimmunoassays. It was observed that PRL serum, pituitary and mRNA concentrations tended to increase near the time of the gonadotropin preovulatory surge, particularly between 24 hrs before behavioral estrus to 5 hours after estrus. However, the changes in PRL mRNA, serum and pituitary concentrations were shown not to be statistically significant. These data suggest that PRL production during the sheep estrous cycle is maintained without dramatic changes in synthesis or secretion.

  11. Published online 14 May 2008 Nucleic Acids Research, 2008, Vol. 36, Web Server issue W97W103 doi:10.1093/nar/gkn280

    E-Print Network [OSTI]

    Lee, Doheon

    algorithms by statistical analysis with the pub- lished experimental data and derived a new efficient method Genomics Research Center, KRIBB, Daejeon 305-806, 2 Department of Bio and Brain Engineering, KAIST, Daejeon and FASTA algo- rithms and checking the folding secondary structure energy of siRNAs. To do this, we

  12. Published online 11 August 2009 Nucleic Acids Research, 2009, Vol. 37, No. 18 61616173 doi:10.1093/nar/gkp597

    E-Print Network [OSTI]

    Glover, Mark

    . N. M. Glover1, * 1 Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7 whom correspondence should be addressed. Tel: +1 780 492 2136; Fax: +1 780 492 0886; Email: mark.glover

  13. W238W244 Nucleic Acids Research, 2007, Vol. 35, Web Server issue doi:10.1093/nar/gkm308

    E-Print Network [OSTI]

    Stormo, Gary

    to biological processes or to disease states, the determination of the transcriptional regulation of these co a comprehensive, user-friendly web application suite termed the Promoter Analysis Pipeline (PAP). PAP is available of the genetic program. Aberrant regulation at different stages of these processes may result in differential

  14. A New Heat Shock Protein That Binds Nucleic Acids* (Received for publication, August 20, 1998, and in revised form, September 28, 1998)

    E-Print Network [OSTI]

    Bardwell, James

    production of a number of proteins termed heat shock proteins (Hsps).1 Although heat shock pro- teins were Hsp15-P2 (5 - TCTTGCAGGATCCAGTTATTCACT-3 ), and Taq polymerase (Strat- agene). The PCR product g, 4 °C). All further steps were performed at 4 °C. The cell pellet was resuspended in cold buffer

  15. Published online 21 April 2009 Nucleic Acids Research, 2009, Vol. 37, No. 10 31253133 doi:10.1093/nar/gkp250

    E-Print Network [OSTI]

    Vologodskii, Alexander

    reactions of break- ing and rejoining DNA strands can be performed without consumption of external energy without energy consumption as well. The energy is required, however, to shift a system away from topoisomerases Alexander Vologodskii* Department of Chemistry, New York University, New York, NY 10003, USA

  16. Process for the preparation of lactic acid and glyceric acid

    DOE Patents [OSTI]

    Jackson, James E [Haslett, MI; Miller, Dennis J [Okemos, MI; Marincean, Simona [Dewitt, MI

    2008-12-02T23:59:59.000Z

    Hexose and pentose monosaccharides are degraded to lactic acid and glyceric acid in an aqueous solution in the presence of an excess of a strongly anionic exchange resin, such as AMBERLITE IRN78 and AMBERLITE IRA400. The glyceric acid and lactic acid can be separated from the aqueous solution. Lactic acid and glyceric acid are staple articles of commerce.

  17. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30T23:59:59.000Z

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  18. 2014;74:3603-3616. Published OnlineFirst May 1, 2014.Cancer Res Man-Li Luo, Chang Gong, Chun-Hau Chen, et al.

    E-Print Network [OSTI]

    Lieberman, Judy

    2014;74:3603-3616. Published OnlineFirst May 1, 2014.Cancer Res Man-Li Luo, Chang Gong, Chun to Promote Cancer Stem­like Cell Traits in Breast Cancer Man-Li Luo1 , Chang Gong4 , Chun-Hau Chen1 , Daniel

  19. Mat. Res. Soc. Symp. Proc. Vol. 628 2000 Materials Research Society Hybrid Inorganic/Organic Diblock Copolymers. Nanostructure in Polyhedral Oligomeric

    E-Print Network [OSTI]

    Mather, Patrick T.

    Our main approach to the synthesis and study of hybrid organic/inorganic materials involvesMat. Res. Soc. Symp. Proc. Vol. 628 © 2000 Materials Research Society CC2.6.1 Hybrid Inorganic the synthesis of melt processable, linear hybrid polymers containing pendent inorganic clusters, and allows us

  20. Published: March 03, 2011 r 2011 American Chemical Society 2538 dx.doi.org/10.1021/pr200015d |J. Proteome Res. 2011, 10, 25382545

    E-Print Network [OSTI]

    Powers, Robert

    a vast number of proteins that lack a functional annotation.1 Many of these unannotated proteins may play. Proteome Res. 2011, 10, 2538­2545 ARTICLE pubs.acs.org/jpr Correlation between Protein Function and Ligand an important role in human disease and, correspondingly, are critical for developing new therapeutics. Protein

  1. 10.1101/gr.6743907Access the most recent version at doi: 2008 18: 188-196; originally published online Nov 19, 2007;Genome Res.

    E-Print Network [OSTI]

    Alvarado, Alejandro Sánchez

    online Nov 19, 2007;Genome Res. Sánchez Alvarado and Mark Yandell Brandi L. Cantarel, Ian Korf, Sofia M for emerging model organism genomes Brandi L. Cantarel,1 Ian Korf,2 Sofia M.C. Robb,3 Genis Parra,2 Eric Ross,4

  2. 10.1101/gr.6743907Access the most recent version at doi: 2008 18: 188-196; originally published online Nov 19, 2007;Genome Res.

    E-Print Network [OSTI]

    Yandell, Mark

    online Nov 19, 2007;Genome Res. et al.Brandi L. Cantarel, Ian Korf, Sofia M.C. Robb, model organism for emerging model organism genomes Brandi L. Cantarel,1 Ian Korf,2 Sofia M.C. Robb,3 Genis Parra,2 Eric Ross,4

  3. Linking promotion strategies for RES-E and CO2 reduction in a liberalised power market: Is a simultaneous policy necessary?

    E-Print Network [OSTI]

    Linking promotion strategies for RES-E and CO2 reduction in a liberalised power market, the influence of the restriction of the greenhouse gas emissions on the electricity generation with respect to the integration of different renewable energy sources into the liberalised European electricity

  4. 10.1101/gr.074492.107Access the most recent version at doi: 2008 18: 821-829 originally published online March 18, 2008Genome Res.

    E-Print Network [OSTI]

    Toronto, University of

    online March 18, 2008Genome Res. Daniel R. Zerbino and Ewan Birney graphs Velvet: Algorithms for de novo short read assembly using de Bruijn Material Supplemental http://genome.cshlp.org/content/suppl/2008/04/08/gr.074492.107.DC1.html References http://genome.cshlp.org/content/18/5/821.full

  5. Amer J of Potato Res (2006) 83:249-257 249 Furrow vs Hill Planting of Sprinkler-Irrigated Russet Burbank

    E-Print Network [OSTI]

    Steele, Dean D.

    2006-01-01T23:59:59.000Z

    Amer J of Potato Res (2006) 83:249-257 249 Furrow vs Hill Planting of Sprinkler-Irrigated Russet Burbank Potatoes on Coarse-Textured Soils Dean D. Steele1 *, Richard G. Greenland2 , and Harlene M Surface water runoff from the hill, where potatoes are planted, to the furrow may exacerbate potato

  6. EMSL - Nuclei acid structure

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    conditions showed a temporary pH decrease, with a concomitant increase in formic acid during exponential growth. Fermentation experiments performed outside of the magnet...

  7. Nuclei acid structure | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    conditions showed a temporary pH decrease, with a concomitant increase in formic acid during exponential growth. Fermentation experiments performed outside of the magnet...

  8. Recovery of organic acids

    DOE Patents [OSTI]

    Verser, Dan W. (Menlo Park, CA); Eggeman, Timothy J. (Lakewood, CO)

    2011-11-01T23:59:59.000Z

    A method is disclosed for the recovery of an organic acid from a dilute salt solution in which the cation of the salt forms an insoluble carbonate salt. A tertiary amine and CO.sub.2 are introduced to the solution to form the insoluble carbonate salt and a complex between the acid and an amine. A water immiscible solvent, such as an alcohol, is added to extract the acid/amine complex from the dilute salt solution to a reaction phase. The reaction phase is continuously dried and a product between the acid and the solvent, such as an ester, is formed.

  9. Recovery of organic acids

    DOE Patents [OSTI]

    Verser, Dan W. (Golden, CO); Eggeman, Timothy J. (Lakewood, CO)

    2009-10-13T23:59:59.000Z

    A method is disclosed for the recovery of an organic acid from a dilute salt solution in which the cation of the salt forms an insoluble carbonate salt. A tertiary amine and CO.sub.2 are introduced to the solution to form the insoluble carbonate salt and a complex between the acid and an amine. A water immiscible solvent, such as an alcohol, is added to extract the acid/amine complex from the dilute salt solution to a reaction phase. The reaction phase is continuously dried and a product between the acid and the solvent, such as an ester, is formed.

  10. Reversible Acid Gas Capture

    ScienceCinema (OSTI)

    Dave Heldebrant

    2012-12-31T23:59:59.000Z

    Pacific Northwest National Laboratory scientist David Heldebrant demonstrates how a new process called reversible acid gas capture works to pull carbon dioxide out of power plant emissions.

  11. Controlling acid rain

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01T23:59:59.000Z

    High concentrations of sulfuric and nitric acid in raTn fn the northeastern USA are caused by the large scale combustion of fossil fuels within this region. Average precipitation acidity is pH 4.2, but spatial and temporal ...

  12. Amer J of Potato Res (2006) 83:241-247 241 Beacon Chipper: A Round White Chip-Processing Potato Variety

    E-Print Network [OSTI]

    Douches, David S.

    2006-01-01T23:59:59.000Z

    Amer J of Potato Res (2006) 83:241-247 241 Beacon Chipper: A Round White Chip-Processing Potato,East Lansing,MI48824,USA 2Dept.of Plant Pathology,MichiganState University,East Lansing,MI48824,USA 3MichiganPotato Industry Commission,13109SchaveyRd, Ste 7, Dewitt,MI48820,USA 4MainePotato Board, 744MainSt.,Room1, Presque

  13. Cysteine-containing peptides having antioxidant properties

    DOE Patents [OSTI]

    Bielicki, John K. (Castro Valley, CA)

    2007-05-15T23:59:59.000Z

    The term "homology" or "homologous" means an amino acid similarity measured by the program, BLAST (Altschul et al (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:33 89 3402), and expressed as --(% identity n/n). In measuring homology between a peptide and a protein of greater size, homology is measured only in the corresponding region; that is, the protein is regarded as only having the same general length as the peptide, allowing for gaps and insertions.

  14. IN MEDIAS RES IN MEDIAS RES

    E-Print Network [OSTI]

    translation of the Roman lyric poet Horace's rule addressed to the creator, the young poet or maker: Semper ad

  15. Focus Sheet | Hydrofluoric Acid Health hazards of hydrofluoric acid

    E-Print Network [OSTI]

    Wilcock, William

    Focus Sheet | Hydrofluoric Acid Health hazards of hydrofluoric acid Hydrofluoric acid (HF characterized by weight loss, brittle bones, anemia, and general ill health. Safe use If possible, avoid working to exposures. #12;Focus Sheet | Hydrofluoric Acid Environmental Health and Safety Environmental Programs Office

  16. A Direct, Biomass-Based Synthesis of Benzoic Acid: Formic Acid-Mediated Deoxygenation of the Glucose-Derived Materials Quinic Acid and Shikimic Acid

    SciTech Connect (OSTI)

    Arceo, Elena; Ellman, Jonathan; Bergman, Robert

    2010-05-03T23:59:59.000Z

    An alternative biomass-based route to benzoic acid from the renewable starting materials quinic acid and shikimic acid is described. Benzoic acid is obtained selectively using a highly efficient, one-step formic acid-mediated deoxygenation method.

  17. Asphaltene damage in matrix acidizing

    E-Print Network [OSTI]

    Hinojosa, Roberto Antonio

    1996-01-01T23:59:59.000Z

    REVIEW CONSTRUCTION OF APPARATUS . DESCRIPTION OF CORE SAMPLES DESIGN OF EXPERIMENTAL ACID TREATMENT . . . ACIDIZING RESULTS BRINE SATURATED CORE L1D ACIDIZING RESULTS BRINE/CRUDE OIL SATURATED CORE S2A . . . ACIDIZING RESULTS BRINE/KEROSENE OIL... experiment they used HCl saturated kerosene to test the same crude samples. Deposition occurred with the HCl saturated acid. The authors concluded, though deposition at an interface was preferential, sludge formation did not require an interface. Moore et...

  18. Optical high acidity sensor

    DOE Patents [OSTI]

    Jorgensen, B.S.; Nekimken, H.L.; Carey, W.P.; O`Rourke, P.E.

    1997-07-22T23:59:59.000Z

    An apparatus and method for determining acid concentrations in solutions having acid concentrations of from about 0.1 Molar to about 16 Molar is disclosed. The apparatus includes a chamber for interrogation of the sample solution, a fiber optic light source for passing light transversely through the chamber, a fiber optic collector for receiving the collimated light after transmission through the chamber, a coating of an acid resistant polymeric composition upon at least one fiber end or lens, the polymeric composition in contact with the sample solution within the chamber and having a detectable response to acid concentrations within the range of from about 0.1 Molar to about 16 Molar, a measurer for the response of the polymeric composition in contact with the sample solution, and a comparer of the measured response to predetermined standards whereby the acid molarity of the sample solution within the chamber can be determined. Preferably, a first lens is attached to the end of the fiber optic light source, the first lens adapted to collimate light from the fiber optic light source, and a second lens is attached to the end of the fiber optic collector for focusing the collimated light after transmission through the chamber. 10 figs.

  19. Synthesis of acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, Luc (Lakewood, CO)

    2003-06-24T23:59:59.000Z

    A process of preparing an acid addition salt of delta-aminolevulinc acid comprising: a) dissolving a lower alkyl 5-bromolevulinate and hexamethylenetetramine in a solvent selected from the group consisting of water, ethyl acetate, chloroform, acetone, ethanol, tetrahydrofuran and acetonitrile, to form a quaternary ammonium salt of the lower alkyl 5-bromolevulinate; and b) hydrolyzing the quaternary ammonium salt with an inorganic acid to form an acid addition salt of delta-aminolevulinic acid.

  20. acid succinic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    simulated the laser heating of the succinic acid (this data is still simulation is that infrared heating generates about 10-15 more succinic acid molecules bound to the analyte...

  1. acid docosahexaenoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 38 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  2. acid aspartic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 20 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  3. acid caffeic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 11 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  4. acid propionic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 19 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  5. acid sorbic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 9 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  6. acid benzoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 24 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  7. acid propanoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 9 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  8. acid methoxyacetic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  9. acid eicosapentaenoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 18 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  10. acids eicosapentaenoic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 18 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  11. acid acetylsalicylic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 10 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  12. acid dichloroacetic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  13. acid oleic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 31 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  14. Recovery of Carboxylic Acids from Fermentation Broth via Acid Springing

    E-Print Network [OSTI]

    Dong, Jipeng

    2010-01-14T23:59:59.000Z

    RECOVERY OF CARBOXYLIC ACIDS FROM FERMENTATION BROTH VIA ACID SPRINGING A Thesis by JIPENG DONG Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree... of MASTER OF SCIENCE December 2008 Major Subject: Chemical Engineering RECOVERY OF CARBOXYLIC ACIDS FROM FERMENTATION BROTH VIA ACID SPRINGING A Thesis by JIPENG DONG Submitted to the Office of Graduate Studies of Texas A...

  15. Acid placement and coverage in the acid jetting process

    E-Print Network [OSTI]

    Mikhailov, Miroslav I.

    2009-05-15T23:59:59.000Z

    Many open-hole acid treatments are being conducted by pumping acid through jetting ports placed at the end of coiled tubing or drill pipe. The filter-cake on the bore-hole is broken by the jet; the acid-soluble material is dissolved, creating...

  16. Method for isolating chromosomal DNA in preparation for hybridization in suspension

    DOE Patents [OSTI]

    Lucas, Joe N. (San Ramon, CA)

    2000-01-01T23:59:59.000Z

    A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration. Chromosomal DNA in a sample containing cell debris is prepared for hybridization in suspension by treating the mixture with RNase. The treated DNA can also be fixed prior to hybridization.

  17. Lubrication with boric acid additives

    DOE Patents [OSTI]

    Erdemir, Ali (Naperville, IL)

    2000-01-01T23:59:59.000Z

    Self-lubricating resin compositions including a boric acid additive and a synthetic polymer including those thermoset materials.

  18. Pantothenic acid biosynthesis in zymomonas

    DOE Patents [OSTI]

    Tao, Luan; Tomb, Jean-Francois; Viitanen, Paul V.

    2014-07-01T23:59:59.000Z

    Zymomonas is unable to synthesize pantothenic acid and requires this essential vitamin in growth medium. Zymomonas strains transformed with an operon for expression of 2-dehydropantoate reductase and aspartate 1-decarboxylase were able to grow in medium lacking pantothenic acid. These strains may be used for ethanol production without pantothenic acid supplementation in seed culture and fermentation media.

  19. Investigating acid rain

    SciTech Connect (OSTI)

    Not Available

    1981-01-01T23:59:59.000Z

    A report is given of an address by Kathleen Bennett, Assistant Administrator of Air, Noise and Radiation, Environmental Protection Agency which was presented to the US Senate Committee on the Environment and Public Works. Bennet explained that in view of the many unknowns about acid rain, and the possible substantial cost burden of additional controls, EPA is proceeding with its program to investigate this environmental malady over a 10-year period. The three major areas of the research program are (1) transport, transformation, and deposition processes, (2) effects of acid deposition, and (3) assessments and policy studies. Other issues discussed were global transboundary air pollution and Senate amendments addressing long-range transport. (JMT)

  20. 284 Chem. Res. Toxicol. 1993,6, 284-293 Enzyme-Linked Immunosorbent Assay for the Specific

    E-Print Network [OSTI]

    Hammock, Bruce D.

    presumably causes lung disease. The mercapturic acid conjugates of naphthalene [NaphMA (l),N-acetyl- S-(1- aromatics in ambient air (4). Naphthalene derivatives selectively produce lesions in the lungs of mice to contribute substan- tially to lung diseases. Naphthalene is considered one

  1. Characterization of co-circulating swine influenza A viruses in North America and the identification of a novel H1 genetic clade with antigenic significance

    E-Print Network [OSTI]

    Anderson, Tavis K.; Campbell, Brian A.; Nelson, Martha I.; Lewis, Nicola S.; Janas-Martindale, Alicia; Killian, Mary Lea; Vincent, Amy L.

    2015-02-18T23:59:59.000Z

    and 1293 N2 neuraminidase gene segments, and 2126 matrix gene 106 segments (Edgar, 2004; Tamura et al., 2011). For each alignment, a maximum likelihood tree was inferred 107 using RAxML (8.0.24; Stamatakis, 2006) on the CIPRES Science Center Gateway... ., Rambaut, A., 2012. Bayesian phylogenetics with BEAUti and 398 the BEAST 1.7. Mol Biol Evol 29, 1969–1973. doi:10.1093/molbev/mss075 399 Edgar, R.C., 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. 400 Nucleic Acids Res...

  2. Hi-Res WIPP

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cn SunnybankD.jpgHanfordDepartment ofHeatHenryDuncan - Atomic ShieldHoll

  3. Fatty Acid Carcass Mapping

    E-Print Network [OSTI]

    Turk, Stacey N.

    2010-01-14T23:59:59.000Z

    calves as yearlings optimizes beef fatty acid composition. M.S. thesis, College Station: Texas A&M University. Chung, K.Y., Lunt, D.K., Choi, C.B., Chae, S.H., Rhoades, R.D., Adams, T.H., Booren, B., & Smith, S.B. (2006). Lipid characteristics... of subcutaneous adipose tissue and M. longissiumus thoracis of Angus and Wagyu steers fed to U.S. and Japanese endpoints. Meat Science, 73(3), 432-441. Chung, K.Y., Lunt, D.K., Kawachi, H., Yano, H., & Smith, S.B. (2005). Stearoyl coenzyme A desaturase...

  4. acid acetic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of asphaltene deposition that occurs during acid treatments of oil reservoirs. Asphaltenes are present to some degree in most hydrocarbons. Due to the molecular weight of the...

  5. J Plant Res (2004) 117:1318 The Botanical Society of Japan and Springer-Verlag Tokyo 2003 Digital Object Identifier (DOI) 10.1007/s10265-003-0121-7

    E-Print Network [OSTI]

    Tomimatsu,, Hiroshi

    2004-01-01T23:59:59.000Z

    J Plant Res (2004) 117:13­18 © The Botanical Society of Japan and Springer-Verlag Tokyo 2003 and Genetics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060­0810, Japan Tel Agency of Japan 2000). Despite the need for conservation, little is known of the life history

  6. Acidic gas capture by diamines

    DOE Patents [OSTI]

    Rochelle, Gary (Austin, TX); Hilliard, Marcus (Missouri City, TX)

    2011-05-10T23:59:59.000Z

    Compositions and methods related to the removal of acidic gas. In particular, the present disclosure relates to a composition and method for the removal of acidic gas from a gas mixture using a solvent comprising a diamine (e.g., piperazine) and carbon dioxide. One example of a method may involve a method for removing acidic gas comprising contacting a gas mixture having an acidic gas with a solvent, wherein the solvent comprises piperazine in an amount of from about 4 to about 20 moles/kg of water, and carbon dioxide in an amount of from about 0.3 to about 0.9 moles per mole of piperazine.

  7. Organic Phosphoric Acid of the Soil.

    E-Print Network [OSTI]

    Fraps, G. S. (George Stronach)

    1911-01-01T23:59:59.000Z

    . ................................................ introduction 5 .............................. hmmonia-Soluble Phosphoric Acid 5 ................ Solubility of Phosphates in Ammonia 6 I Fixation of Phosphoric Acid from Ammonia .......... 7 Effect of Ratio of Soil to Solvent in Extraction of Phos- I I... .............. phoric Acid by Acid and Ammonia 7 I ........ Other Soil Constituents Dissolved by Ammonia 8 ................... Solution of Fixed Phosphoric Acid 10 ................ ormation of Ammonia-Solubla Phosphoric Acid 11 ....... hosphoric Acid Dissolved...

  8. acidic alpha-amino acids: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acid indicated that more succinic acid... Gilliland, Patti Lynn 2012-06-07 24 A ACID RAIN Audrey Gibson Geosciences Websites Summary: , oxygen, and oxidants to form...

  9. Carbonic Acid Pretreatment of Biomass

    SciTech Connect (OSTI)

    G. Peter van Walsum; Kemantha Jayawardhana; Damon Yourchisin; Robert McWilliams; Vanessa Castleberry

    2003-05-31T23:59:59.000Z

    This project sought to address six objectives, outlined below. The objectives were met through the completion of ten tasks. 1) Solidify the theoretical understanding of the binary CO2/H2O system at reaction temperatures and pressures. The thermodynamics of pH prediction have been improved to include a more rigorous treatment of non-ideal gas phases. However it was found that experimental attempts to confirm theoretical pH predictions were still off by a factor of about 1.8 pH units. Arrhenius experiments were carried out and the activation energy for carbonic acid appears to be substantially similar to sulfuric acid. Titration experiments have not yet confirmed or quantified the buffering or acid suppression effects of carbonic acid on biomass. 2) Modify the carbonic acid pretreatment severity function to include the effect of endogenous acid formation and carbonate buffering, if necessary. It was found that the existing severity functions serve adequately to account for endogenous acid production and carbonate effects. 3) Quantify the production of soluble carbohydrates at different reaction conditions and severity. Results show that carbonic acid has little effect on increasing soluble carbohydrate concentrations for pretreated aspen wood, compared to pretreatment with water alone. This appears to be connected to the release of endogenous acids by the substrate. A less acidic substrate such as corn stover would derive benefit from the use of carbonic acid. 4) Quantify the production of microbial inhibitors at selected reaction conditions and severity. It was found that the release of inhibitors was correlated to reaction severity and that carbonic acid did not appear to increase or decrease inhibition compared to pretreatment with water alone. 5) Assess the reactivity to enzymatic hydrolysis of material pretreated at selected reaction conditions and severity. Enzymatic hydrolysis rates increased with severity, but no advantage was detected for the use of carbonic acid compared to water alone. 6) Determine optimal conditions for carbonic acid pretreatment of aspen wood. Optimal severities appeared to be in the mid range tested. ASPEN-Plus modeling and economic analysis of the process indicate that the process could be cost competitive with sulfuric acid if the concentration of solids in the pretreatment is maintained very high (~50%). Lower solids concentrations result in larger reactors that become expensive to construct for high pressure applications.

  10. A ACID RAIN Audrey Gibson

    E-Print Network [OSTI]

    Toohey, Darin W.

    acid and nitric acid. Sunlight increases the rate of most of these reactions. Electric utility plants;Gas Natural Sources Concentration Carbon dioxide CO2 Decomposition 355 ppm Nitric oxide NO Electric, 2010 #12;Gas Non-Natural Sources Concentration Nitric oxide NO Internal Combustion (cars) 0.2 ppm

  11. Metabolism of Thioctic Acid in Algae

    E-Print Network [OSTI]

    Grisebach, Hans; Fuller, R.C.; Calvin, M.

    1956-01-01T23:59:59.000Z

    METABOLISM OF THlOCTlC ACID IN ALGAE TWO-WEEK LOAN COPY ThisMETABOLISM OF THIOCTIC ACID IN ALGAE Hans Grisebach, R. , C.METABOLISM OF THIOCTIC ACID IN ALGAE Hans Grisebach, R. C.

  12. acetic acid solutions: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  13. arachidonic acid activation: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  14. acid inertness studies: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  15. acid alleviates decreases: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  16. acid activated montmorillonite: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  17. acid amide hydrolase: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been studied....

  18. acid chelation phototherapeutic: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  19. acid phosphatase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  20. acetic acid solution: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  1. acid 2-benzothiazolylthiomethyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  2. acetic acid operational: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  3. acid phosphatase activities: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  4. acid sphingomyelinase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  5. acids decreases fibrinolysis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  6. acid potassium glycyrrhetinate: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  7. arachidonic acid activates: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  8. acid decarboxylase activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  9. acid activates nrf2: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  10. acid processing activity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  11. ascorbic acid enhances: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  12. acid incorporating poloxamer: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  13. acid cupric chloride: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  14. acids increase cardiovascular: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In the present study, bonding among formic, acetic and benzoic acids, sulfuric acid, ammonia, acetic, and benzoic acids with free and hydrated sulfuric acid has been...

  15. In vivo incorporation of unnatural amino acids

    DOE Patents [OSTI]

    Schultz, Peter G. (La Jolla, CA); Wang, Lei (San Diego, CA); Anderson, John Christopher (San Diego, CA); Chin, Jason W. (Cambridge, GB); Liu, David R. (Lexington, MA); Magliery, Thomas J. (North Haven, CT); Meggers, Eric L. (Philadelphia, PA); Mehl, Ryan Aaron (Lancaster, PA); Pastrnak, Miro (San Diego, CA); Santoro, Stephen William (Cambridge, MA); Zhang, Zhiwen (San Diego, CA)

    2012-05-08T23:59:59.000Z

    The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

  16. Carbonic Acid Shows Promise in Geology, Biology

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Surprising Secrets of Carbonic Acid Probing the Surprising Secrets of Carbonic Acid Berkeley Lab Study Holds Implications for Geological and Biological Processes October 23,...

  17. Controlling acid rain : policy issues

    E-Print Network [OSTI]

    Fay, James A.

    1983-01-01T23:59:59.000Z

    The policy and regulatory ramifications of U.S. acid rain control programs are examined; particularly, the alternative of a receptor-oriented strategy as constrasted to emission-oriented proposals (e.g., the Mitchell bill) ...

  18. Nitrate and Prussic Acid Poisoning

    E-Print Network [OSTI]

    Stichler, Charles; Reagor, John C.

    2001-09-05T23:59:59.000Z

    Nitrate and prussic acid poisoning in cattle are noninfectious conditions that can kill livestock. This publication explains the causes and symptoms of these conditions as well as preventive measures and sampling and testing steps....

  19. Seasonalepisodic control of acid deposition

    E-Print Network [OSTI]

    Fay, James A.

    1988-01-01T23:59:59.000Z

    This report contains the climatological, technical and economic factors for episodic and seasonal control of emissions in existing power plants. Analyzing a large data set of acid deposition for the years 1982-85, we find ...

  20. Nitrate and Prussic Acid Poisoning 

    E-Print Network [OSTI]

    Stichler, Charles; Reagor, John C.

    2001-09-05T23:59:59.000Z

    Nitrate and prussic acid poisoning in cattle are noninfectious conditions that can kill livestock. This publication explains the causes and symptoms of these conditions as well as preventive measures and sampling and testing steps....

  1. High-Fidelity DNA Hybridization Using Programmable Molecular DNA Devices

    E-Print Network [OSTI]

    Reif, John H.

    of complementary nucleic acid strands is the most basic of all reactions involving nucleic acids, but has a major specific high-fidelity DNA hybridization reactions for tar- get strands of arbitrary length. Our protocol acid strands is the most basic of all reactions involving nucleic acids and a major component of most

  2. Citation: Molecular Therapy--Nucleic Acids (2013) 2, e107; doi:10.1038/mtna.2013.37 2013 The American Society of Gene & Cell Therapy All rights reserved 2162-2531/12

    E-Print Network [OSTI]

    Cai, Long

    2013-01-01T23:59:59.000Z

    drugs that have serious systemic toxicities, such as doxorubicin (Dox). Dox is among the most widely.37 Treatment with doxorubicin (Dox) results in serious systemic toxicities that limit effectiveness for cancer-specific membrane antigen (PSMA) using fixed sequences to promote Dox binding and developed dimeric aptamer

  3. University of California, Irvine Environmental Health and Safety www.ehs.uci.edu Questions Call: (949) 824-6200 Version 2.1 Ethidium bromide is a commonly used stain for identifying nucleic acids in electrophoresis gels. It is

    E-Print Network [OSTI]

    George, Steven C.

    &S hazardous waste label and placed in secondary containment. Do not use a biohazardous waste bag to package bromide solutions must be treated as part of the experimental protocol or managed as a hazardous chemical is saturated, the charcoal must be managed as a hazardous chemical waste and disposed of through EH&S. Charcoal

  4. Succinic acid production by Anaerobiospirillum succiniciproducens

    E-Print Network [OSTI]

    , succinic acid has been produced commercially by chemical processes. Recently, however, fermentative of bacteria produce succinic acid as a fermentation end product,4 7 few species can produce it as the major 10 Previous studies showed that A. succiniciproducens produces succinic acid and acetic acid

  5. Process for the reclamation of battery acid and fluid from expended lead-acid batteries

    SciTech Connect (OSTI)

    Spitz, R.A.

    1990-11-20T23:59:59.000Z

    This patent describes a method for recycling contaminated sulfuric acid from lead acid batteries to reclaimed sulfuric acid fore reuse in the batteries by removing contaminating iron impurities. It comprises: diluting the contaminated sulfuric acid to a concentration between 150 and 230 grams per liter; filtering the sulfuric acid through a first filter means to remove solid impurities.

  6. Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants

    DOE Patents [OSTI]

    Ohlrogge, John B. (Okemos, MI); Cahoon, Edgar B. (Lansing, MI); Shanklin, John (Upton, NY); Somerville, Christopher R. (Okemos, MI)

    1995-01-01T23:59:59.000Z

    The present invention relates to a process for producing lipids containing the fatty acid petroselinic acid in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a .omega.12 desaturase from another species which does normally accumulate petroselinic acid.

  7. Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants

    DOE Patents [OSTI]

    Ohlrogge, J.B.; Cahoon, E.B.; Shanklin, J.; Somerville, C.R.

    1995-07-04T23:59:59.000Z

    The present invention relates to a process for producing lipids containing the fatty acid, petroselinic acid, in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a {omega}12 desaturase from another species which does normally accumulate petroselinic acid. 19 figs.

  8. The integrated microbial genomes (IMG) system in 2007: data content and analysis tool extensions

    E-Print Network [OSTI]

    2008-01-01T23:59:59.000Z

    for genome annotation: PRIAM. Nucleic Acids Research 31 (EC numbers are computed using PRIAM (13), as a complement to

  9. RRDistMaps: a UCSF Chimera tool for viewing and comparing protein distance maps.

    E-Print Network [OSTI]

    Chen, JE; Huang, CC; Ferrin, TE

    2015-01-01T23:59:59.000Z

    Enhancing UCSF Chimera through web services. Nucleic Acids2004) via an online RBVI web service (Huang, 2014). In both

  10. Multiplex automated genome engineering

    DOE Patents [OSTI]

    Church, George M; Wang, Harris H; Isaacs, Farren J

    2013-10-29T23:59:59.000Z

    The present invention relates to automated methods of introducing multiple nucleic acid sequences into one or more target cells.

  11. HYDROFLUORIC ACID FIRST AID INSTRUCTIONS

    E-Print Network [OSTI]

    Jalali. Bahram

    with large amounts of cool running water. Immediately washing off the acid is of primary importance. 2.Remove Immediately flush eyes for at least 15 minutes with copious cool flowing water. 2 If only one eye is affected by a glass of milk or milk of magnesia. 3 Call 911 for immediate medical assistance. REMEMBER, ALL PERSONNEL

  12. Instrument Application Capabilities Advantages Disadvantages Recommendations Contact Qiagen Qiacube

    E-Print Network [OSTI]

    Cui, Yan

    @uthsc.edu Agilent Bioanalyzer Quality control Profiles for total RNA; enriched mRNA; enriched small RNA; DNA Nucleic acid isolation and purification Total RNA; miRNA; DNA; All prep (all nucleic acid) 2-12 samples Felicia Waller fwaller@uthsc.edu Nanodrop Spectrophotometer Nucleic acids quantification and quality

  13. Biomedical Microdevices 7:1, 712, 2005 C 2005SpringerScience+BusinessMedia, Inc. ManufacturedinTheNetherlands.

    E-Print Network [OSTI]

    Boppart, Stephen

    hybridization to its tar- get nucleic acid (Tyagi et al., 1998; Tyagi and Kramer 1996 hybridization to a target nucleic acid, can be used in microflu- idic devices to detect and quantify nucleic acids in solution as well as inside bacterial cells. Three essential steps towards the development

  14. JOURNAL OF VIROLOGY, 0022-538X/98/$04.00 0

    E-Print Network [OSTI]

    Levin, Judith G.

    (a nucleic acid chaperone catalyzing nucleic acid rearrangements which lead to more thermodynamically stable conformations) dramatically stimulates HIV-1 minus-strand transfer by pre- venting TAR conclude that actinomycin D inhibits minus-strand transfer by blocking the nucleic acid chaperone activity

  15. Methods for making nucleotide probes for sequencing and synthesis

    DOE Patents [OSTI]

    Church, George M; Zhang, Kun; Chou, Joseph

    2014-07-08T23:59:59.000Z

    Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.

  16. 3284 E Exobiology (theoretical), Complexity in Exobiology (theoretical),

    E-Print Network [OSTI]

    Brandenburg, Axel

    to as enzymes. Nucleotides and nucleic acids Nucleotides are mono- mers of nucleic acids, e. g., of RNA bases) that can pair in a specific way. Nucleotides can form polymers, and their sequence carries water molecule is removed in this step. Other nucleotides of interest include peptide nucleic acid

  17. acidization: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  18. acids: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  19. Acid Ions are More Than Spectators

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    gas-phase studies. The discovery suggests that it is not simply the release of protons - hydrogen ions - that is important for the properties of acids. When a strong acid, such as...

  20. Recovery of mercury from acid waste residues

    DOE Patents [OSTI]

    Greenhalgh, W.O.

    1987-02-27T23:59:59.000Z

    Mercury can be recovered from nitric acid-containing fluids by reacting the fluid with aluminum metal to produce mercury metal, and thence quenching the reactivity of the nitric acid prior to nitration of the mercury metal. 1 fig.

  1. Fuel cell electrolyte membrane with acidic polymer

    DOE Patents [OSTI]

    Hamrock, Steven J. (Stillwater, MN); Larson, James M. (Saint Paul, MN); Pham, Phat T. (Little Canada, MN); Frey, Matthew H. (Cottage Grove, MN); Haugen, Gregory M. (Edina, MN); Lamanna, William M. (Stillwater, MN)

    2009-04-14T23:59:59.000Z

    An electrolyte membrane is formed by an acidic polymer and a low-volatility acid that is fluorinated, substantially free of basic groups, and is either oligomeric or non-polymeric.

  2. amino acid intake: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    protein intake (PDI) and net portal appearance rate of amino acids by continuous infusion of para-aminohippuric acid via the mesenteric catheter. The amino-acid appearance...

  3. Hydrogen-Bond Acidic Polymers for Chemical Vapor Sensing. | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acidic Polymers for Chemical Vapor Sensing. Hydrogen-Bond Acidic Polymers for Chemical Vapor Sensing. Abstract: A review with 171 references. Hydrogen-bond acidic polymers for...

  4. Reactions Between Water Soluble Organic Acids and Nitrates in...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Between Water Soluble Organic Acids and Nitrates in Atmospheric Aerosols: Recycling of Nitric Acid and Formation of Reactions Between Water Soluble Organic Acids and Nitrates in...

  5. acid anew insights: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    is the sequence of amino acids produced, and because amino acids average about Texas at San Antonio, University of 290 Combined Acid Catalysis for Asymmetric Synthesis Chemistry...

  6. acid synthase impacts: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acid utilization and glucose oxidation. Glucose... Adhikari, Sean 2006-10-30 246 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  7. Chemical Additive Selection in Matrix Acidizing 

    E-Print Network [OSTI]

    Weidner, Jason 1981-

    2011-05-09T23:59:59.000Z

    critical detail of weak acid chemistry. One concern when using any acid in oilfield operations is the corrosion of well tubulars. Thus operators often choose to pump corrosion inhibitor, a chemical additive electrostatically attracted... to the negative charge of the well casing or production tubing, to decrease the rate at which the acid accesses well tubular surfaces (Crowe and Minor 1985). A typical working concentration of corrosion inhibitor is 1-2 wt% of injected acid (Smith et al. 1978...

  8. Organic Acid Production by Filamentous Fungi

    E-Print Network [OSTI]

    -being. Indeed, organic acid fermentations are often not even identified as fungal bioprocesses, having been Aspergillus niger in aerated stirred-tank-reactors can convert glucose to citric acid with greater than 80 lipolytica, and related yeast species, may be in use commercially to produce citric acid (Lopez-Garcia, 2002

  9. Unnatural reactive amino acid genetic code additions

    DOE Patents [OSTI]

    Deiters, Alexander (La Jolla, CA); Cropp, T. Ashton (Bethesda, MD); Chin, Jason W. (Cambridge, GB); Anderson, J. Christopher (San Francisco, CA); Schultz, Peter G. (La Jolla, CA)

    2011-08-09T23:59:59.000Z

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNAsyn-thetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  10. Unnatural reactive amino acid genetic code additions

    DOE Patents [OSTI]

    Deiters, Alexander; Cropp, Ashton T; Chin, Jason W; Anderson, Christopher J; Schultz, Peter G

    2013-05-21T23:59:59.000Z

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  11. Unnatural reactive amino acid genetic code additions

    DOE Patents [OSTI]

    Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.; Anderson, J. Christopher; Schultz, Peter G.

    2014-08-26T23:59:59.000Z

    This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

  12. Synthesis of an acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, Luc (Lakewood, CO)

    1999-01-01T23:59:59.000Z

    A process of preparing an acid addition salt of delta-aminolevulinic acid comprising: dissolving a lower alkyl 5-bromolevulinate and an alkali metal diformylamide in an organic solvent selected from the group consisting of acetonitrile, methanol, tetrahydrofuran, 2-methyltetrahydrofuran and methylformate or mixtures thereof to form a suspension of an alkyl 5-(N,N-diformylamino) levulinate ester; and hydrolyzing said alkyl 5-(N,N-diformylamino) levulinate with an inorganic acid to form an acid addition salt of delta-amino levulinic acid.

  13. Synthesis of an acid addition salt of delta-aminolevulinic acid from 5-bromo levulinic acid esters

    DOE Patents [OSTI]

    Moens, L.

    1999-05-25T23:59:59.000Z

    A process is disclosed for preparing an acid addition salt of delta-aminolevulinic acid comprising. The process involves dissolving a lower alkyl 5-bromolevulinate and an alkali metal diformylamide in an organic solvent selected from the group consisting of acetonitrile, methanol, tetrahydrofuran, 2-methyltetrahydrofuran and methylformate or mixtures to form a suspension of an alkyl 5-(N,N-diformylamino) levulinate ester; and hydrolyzing the alkyl 5-(N,N-diformylamino) levulinate with an inorganic acid to form an acid addition salt of delta-amino levulinic acid.

  14. CHAPTER 13. ACID RAIN Acid rain was discovered in the 19th century by Robert Angus

    E-Print Network [OSTI]

    Jacob, Daniel J.

    247 CHAPTER 13. ACID RAIN Acid rain was discovered in the 19th century by Robert Angus Smith, a pharmacist from Manchester (England), who measured high levels of acidity in rain falling over industrial decline of fish populations in the lakes of southern Norway and traced the problem to acid rain. Similar

  15. Fate of Acids in Clouds 1. Combination with bases dissolved in clouds: acids neutralized

    E-Print Network [OSTI]

    Schofield, Jeremy

    problems. E#11;ects of Acid Rain 1. Vegetation: SO 2 is toxic to plants #15; Leaves damaged below pH 3 rain { Athens and Rome cathedrals and statues: pollution leads to acid rain #15; SteelFate of Acids in Clouds 1. Combination with bases dissolved in clouds: acids neutralized NH 3 (g

  16. Evaluation of acid fracturing based on the "acid fracture number" concept

    E-Print Network [OSTI]

    Alghamdi, Abdulwahab

    2006-08-16T23:59:59.000Z

    ................................................................................................. 29 4.2.1 Initial Pad Volume ........................................................................... 29 4.2.2 Acid Strength and Volume...............................................................30 V... stages of pad fluids and acids.11 The reaction of HCl with carbonate formations is fast, especially at high temperatures. This means that the acid will not be able to penetrate deeply down the fracture, which may affect the outcome of acid fracturing...

  17. Solid-state actinide acid phosphites from phosphorous acid melts

    SciTech Connect (OSTI)

    Oh, George N. [Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Burns, Peter C., E-mail: pburns@nd.edu [Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 156 Fitzpatrick Hall, Notre Dame, IN 46556 (United States); Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556 (United States)

    2014-07-01T23:59:59.000Z

    The reaction of UO{sub 3} and H{sub 3}PO{sub 3} at 100 °C and subsequent reaction with dimethylformamide (DMF) produces crystals of the compound (NH{sub 2}(CH{sub 3}){sub 2})[UO{sub 2}(HPO{sub 2}OH)(HPO{sub 3})]. This compound crystallizes in space group P2{sub 1}/n and consists of layers of uranyl pentagonal bipyramids that share equatorial vertices with phosphite units, separated by dimethylammonium. In contrast, the reaction of phosphorous acid and actinide oxides at 210 °C produces a viscous syrup. Subsequent dilution in solvents and use of standard solution-state methods results in the crystallization of two polymorphs of the actinide acid phosphites An(HPO{sub 2}OH){sub 4} (An=U, Th) and of the mixed acid phosphite–phosphite U(HPO{sub 3})(HPO{sub 2}OH){sub 2}(H{sub 2}O)·2(H{sub 2}O). ?- and ?-An(HPO{sub 2}OH){sub 4} crystallize in space groups C2/c and P2{sub 1}/n, respectively, and comprise a three-dimensional network of An{sup 4+} cations in square antiprismatic coordination corner-sharing with protonated phosphite units, whereas U(HPO{sub 3})(HPO{sub 2}OH){sub 2}(H{sub 2}O){sub 2}·(H{sub 2}O) crystallizes in a layered structure in space group Pbca that is composed of An{sup 4+} cations in square antiprismatic coordination corner-sharing with protonated phosphites and water ligands. We discuss our findings in using solid inorganic reagents to produce a solution-workable precursor from which solid-state compounds can be crystallized. - Graphical abstract: Reaction of UO{sub 3} and H{sub 3}PO{sub 3} at 100 °C and subsequent reaction with DMF produces crystals of (NH{sub 2}(CH{sub 3}){sub 2})[UO{sub 2}(HPO{sub 2}OH)(HPO{sub 3})] with a layered structure. Reaction of phosphorous acid and actinide oxides at 210 °C produces a viscous syrup and further solution-state reactions result in the crystallization of the actinide acid phosphites An(HPO{sub 2}OH){sub 4} (An=U, Th), with a three-dimensional network structure, and the mixed acid phosphite–phosphite U(HPO{sub 3})(HPO{sub 2}OH){sub 2}(H{sub 2}O){sub 2}·(H{sub 2}O) with a layered structure. - Highlights: • U(VI), U(IV) and Th(IV) phosphites were synthesized by solution-state methods. • A new uranyl phosphite structure is based upon uranyl phosphite anionic sheets. • New U and Th phosphites have framework structures.

  18. Micro-electro-mechanical systems phosphoric acid fuel cell

    DOE Patents [OSTI]

    Sopchak, David A. (Livermore, CA); Morse, Jeffrey D. (Martinez, CA); Upadhye, Ravindra S. (Pleasanton, CA); Kotovsky, Jack (Oakland, CA); Graff, Robert T. (Modesto, CA)

    2010-12-21T23:59:59.000Z

    A phosphoric acid fuel cell system comprising a porous electrolyte support, a phosphoric acid electrolyte in the porous electrolyte support, a cathode electrode contacting the phosphoric acid electrolyte, and an anode electrode contacting the phosphoric acid electrolyte.

  19. Micro-electro-mechanical systems phosphoric acid fuel cell

    DOE Patents [OSTI]

    Sopchak, David A. (Livermore, CA); Morse, Jeffrey D. (Martinez, CA); Upadhye, Ravindra S. (Pleasanton, CA); Kotovsky, Jack (Oakland, CA); Graff, Robert T. (Modesto, CA)

    2010-08-17T23:59:59.000Z

    A phosphoric acid fuel cell system comprising a porous electrolyte support, a phosphoric acid electrolyte in the porous electrolyte support, a cathode electrode contacting the phosphoric acid electrolyte, and an anode electrode contacting the phosphoric acid electrolyte.

  20. acid bacteria isolates: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acids are solids not liquids. They sublime under vacuum to compare the strengths of solid acids with liquid acids therefore led us to obtain a measure of acidity in dilute...

  1. acid bacteria isolated: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acids are solids not liquids. They sublime under vacuum to compare the strengths of solid acids with liquid acids therefore led us to obtain a measure of acidity in dilute...

  2. The utilization of tricarboxylic acid cycle acids and the uptake of succinic acid by Neurospora crassa

    E-Print Network [OSTI]

    Gilliland, Patti Lynn

    1978-01-01T23:59:59.000Z

    ~50 V E 0 40 V V Z 30 V Ih 'a 20 0 0 V 10 LEGEND ?, pH 4. l --~pH 7A) 0 0 8 12 16 20 TIME, minutes Figure 1. Time Course of Uptake and Accumulation of Succinic Acid at pH 4. 1 and pH 7. 0 (25'C). Each point represents means for data... to 20 140 pH 4. 1 25 C pH 7. 0 g 120 F100 I ~sc E ef 80 Z V lJ ul 40 0 p 40 2 Z 3'C 20 0 0 5 10 15 20 0 5 10 15 20 TIME, minutes Figure 2. Effect of Temperature on Uptake and Accumulation of Succinic Acid. External concentration...

  3. Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting

    DOE Patents [OSTI]

    Chen, Fanqing Frank; Liu, Gang L; Lee, Luke P

    2013-10-29T23:59:59.000Z

    This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

  4. Solvent extraction of inorganic acids

    E-Print Network [OSTI]

    Ysrael, Miguel Curie

    1965-01-01T23:59:59.000Z

    Page 10 27 NOi'!: KCL ITU8l: BIBLIOG. :l PI!Y ct:"P. '. t:OI:t 28 30 32 LIDT DF FIGUP, . " Al'!D T?DL. '. I F igure . olubility curve of wat-r and glycerol etl'er s I-'age 4 3 ~ '=quilibrium diagram for amyl alcohol- water-HC1... Correlation of eq;ilibrium da!. a of amyl alcohol-water-HC1 12 13 ~ ~ selectivity diagram for alcohol- 5 ~, '~electivity diagram for ethers G. Mole fr:ction di=tribution diagrav. . 7 ~ Activity coefficient of acids in water 0 ~ "ctivity coefficient...

  5. ARM - Lesson Plans: Acid Rain

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625govInstrumentstdmadap Documentation TDMADAP : XDC documentationBarrow, Alaska Outreach Home RoomPlans OutreachAcid

  6. Published: July 05, 2011 r 2011 American Chemical Society 1231 dx.doi.org/10.1021/tx200090n |Chem. Res. Toxicol. 2011, 24, 12311241

    E-Print Network [OSTI]

    Suh, Young-Ger

    ), a representa- tive J-series cyclopentenone prostaglandin, is one of the major terminal products of the cyclooxygenase-2 (COX-2)-dependent arachidonic acid cascade.1 Its synthesis and release can be stimulated during,-unsaturated carbonyl group. In the present study, treatment of human breast cancer MCF-7 cells with 15d- PGJ2 caused

  7. Novel Regenerated Solvent Extraction Processes for the Recovery of Carboxylic Acids or Ammonia from Aqueous Solutions Part I. Regeneration of Amine-Carboxylic Acid Extracts

    E-Print Network [OSTI]

    Poole, L.J.

    2008-01-01T23:59:59.000Z

    production of citric acid by fermentation, recovery of theof Citric Acid from Aqueous Fermentation Solutions byof citric acid was 1.1.1 Lactic Acid Currently, fermentation

  8. HPSS_Cover_Low_Res

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville Power AdministrationField8,Dist.NewofGeothermal848 Unlimited Release PrintedDeputy

  9. RES Anatolia | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof Energy 2,AUDITCalifornia Sector: Wind energy Product:Anatolia Jump to: navigation,

  10. Eole RES | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof Energy 2,AUDIT REPORTEnergy OffshoreDeveloperEnertech JumpEnvironmentalEnvisionEole

  11. PREDICTING TEMPERATURE BEHAVIOR IN CARBONATE ACIDIZING TREATMENTS

    E-Print Network [OSTI]

    Tan, Xuehao

    2010-01-16T23:59:59.000Z

    is the temperature of injected acid. A program is developed to solve the energy balance equation numerically and the flow chart is shown below (Fig. 2.4). 17 Fig. 2.4?Flow chart for programming acid injection problem Start Time Step, p.................................................. 8 2.3 Core flow test results. Pore volumes to breakthrough as a function of injection rate. (Buijse and Glasbergen 2005) ........................................... 13 2.4 Flow chart for programming acid injection problem...

  12. Carboxylic acid accelerated formation of diesters

    DOE Patents [OSTI]

    Tustin, G.C.; Dickson, T.J.

    1998-04-28T23:59:59.000Z

    This invention pertains to accelerating the rate of formation of 1,1-dicarboxylic esters from the reaction of an aldehyde with a carboxylic acid anhydride or a ketene in the presence of a non-iodide containing a strong Bronsted acid catalyst by the addition of a carboxylic acid at about one bar pressure and between about 0 and 80 C in the substantial absence of a hydrogenation or carbonylation catalyst.

  13. Acid rain information book. Draft final report

    SciTech Connect (OSTI)

    None

    1980-12-01T23:59:59.000Z

    Acid rain is one of the most widely publicized environmental issues of the day. The potential consequences of increasingly widespread acid rain demand that this phenomenon be carefully evaluated. Reveiw of the literature shows a rapidly growing body of knowledge, but also reveals major gaps in understanding that need to be narrowed. This document discusses major aspects of the acid rain phenomenon, points out areas of uncertainty, and summarizes current and projected research by responsible government agencies and other concerned organizations.

  14. Solid phase sequencing of biopolymers

    DOE Patents [OSTI]

    Cantor, Charles (Del Mar, CA); Koster, Hubert (La Jolla, CA)

    2010-09-28T23:59:59.000Z

    This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

  15. Acid Doped Membranes for High Temperature PEMFC

    Broader source: Energy.gov [DOE]

    Presentation on Acid Doped Membranes for High Temperature PEMFC to the High Temperature Membrane Working Group, May 25, 2004 in Philadelphia, PA.

  16. acid-dependent ribonucleic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 40 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  17. acid n-glycolylneuraminic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    by USDA and U of I researchers Illinois at Urbana-Champaign, University of 7 Controlling acid rain MIT - DSpace Summary: High concentrations of sulfuric and nitric acid in raTn fn...

  18. Decomposition Studies of Triphenylboron, Diphenylborinic Acid and Phenylboric Acid in Aqueous Alkaline Solutions Containing Copper

    SciTech Connect (OSTI)

    Crawford, C.L. [Westinghouse Savannah River Company, AIKEN, SC (United States); Peterson, R. A.

    1997-02-11T23:59:59.000Z

    This report documents the copper-catalyzed chemical kinetics of triphenylboron, diphenylborinic acid and phenylboric acid (3PB, 2PB and PBA) in aqueous alkaline solution contained in carbon-steel vessels between 40 and 70 degrees C.

  19. The East Penn process for recycling sulfuric acid from lead-acid batteries

    SciTech Connect (OSTI)

    Leiby, R.; Bricker, M. [East Penn Manufacturing Co., Inc., Lyon Station, PA (United States); Spitz, R. [Spitz (R.), Holbrook, MA (United States)

    1995-12-31T23:59:59.000Z

    Prior to March 1992, the only component of the lead-acid battery that was not recycled by East Penn Manufacturing Company was the sulfuric acid electrolyte. This acid was unusable in new batteries because the iron level was found to exceed new product specifications. The development of a liquid ion exchange process to remove the iron from the acid allows East Penn to currently recover over three million gallons of sulfuric acid annually. The process is based upon the use of an iron selective liquid ion exchange material or solvent to extract iron from the sulfuric acid electrolyte followed by regeneration of the solvent. Equilibrium and kinetic data for the extraction and regeneration steps were collected in order to scale up the process to commercial scale. An electrochemical process for the treatment of the acid used in the regeneration step was also developed which significantly reduces the volume of strip acid required in the process.

  20. Effects of Acid Additives on Spent Acid Flowback through Carbonate Cores

    E-Print Network [OSTI]

    Nasir, Ehsaan Ahmad

    2012-07-16T23:59:59.000Z

    these challenges, different chemicals, or additives, are added to the acid solution such as corrosion inhibitors and iron control agents. These additives may change the relative permeability of the spent acid, and formation wettability, and may either hinder...

  1. A method to attenuate U(VI) mobility in acidic waste plumes using humic acids

    E-Print Network [OSTI]

    Wan, J.

    2011-01-01T23:59:59.000Z

    base properties of a goethite surface model: A theoreticalcomplexation of U(VI) on goethite (alpha-FeOOH). Geochim.acid and humic-acid on goethite, gibbsite and imogolite. J.

  2. BNL Citric Acid Technology: Pilot Scale Demonstration

    SciTech Connect (OSTI)

    FRANCIS, A J; DODGE,; J, C; GILLOW, J B; FORRESTER, K E

    1999-09-24T23:59:59.000Z

    The objective of this project is to remove toxic metals such as lead and cadmium from incinerator ash using the Citric Acid Process developed at Brookhaven National Laboratory. In this process toxic metals in bottom ash from the incineration of municipal solid waste were first extracted with citric acid followed by biodegradation of the citric acid-metal extract by the bacterium Pseudomonas fluorescens for metals recovery. The ash contained the following metals: Al, As, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Se, Sr, Ti, and Zn. Optimization of the Citric Acid Process parameters which included citric acid molarity, contact time, the impact of mixing aggressiveness during extraction and pretreatment showed lead and cadmium removal from incinerator ash of >90%. Seeding the treated ash with P. fluorescens resulted in the removal of residual citric acid and biostabilization of any leachable lead, thus allowing it to pass EPA?s Toxicity Characteristic Leaching Procedure. Biodegradation of the citric acid extract removed >99% of the lead from the extract as well as other metals such as Al, Ca, Cu, Fe, Mg, Mn, Ti, and Zn. Speciation of the bioprecipitated lead by Extended X-ray Absorption Fine Structure at the National Synchrotron Light Source showed that the lead is predominantly associated with the phosphate and carboxyl functional groups in a stable form. Citric acid was completely recovered (>99%) from the extract by sulfide precipitation technique and the extraction efficiency of recovered citric acid is similar to that of the fresh citric acid. Recycling of the citric acid should result in considerable savings in the overall treatment cost. We have shown the potential application of this technology to remove and recover the metal contaminants from incinerator ash as well as from other heavy metal bearing wastes (i.e., electric arc furnace dust from steel industry) or soils. Information developed from this project is being applied to demonstrate the remediation of lead paint contaminated soils on Long Island.

  3. Please cite this article in press as: A.P. Mller, et al., High frequency of albinism and tumours in free-living birds around Chernobyl, Mutat. Res.: Genet. Toxicol. Environ. Mutagen. (2013), http://dx.doi.org/10.1016/j.mrgentox.2013.04.019

    E-Print Network [OSTI]

    Mousseau, Timothy A.

    in free-living birds around Chernobyl, Mutat. Res.: Genet. Toxicol. Environ. Mutagen. (2013), http.elsevier.com/locate/mutres High frequency of albinism and tumours in free-living birds around Chernobyl A.P. Møllera, , A Keywords: Albinism Chernobyl Population decline Radiation Tumours a b s t r a c t The effects

  4. Nitrates and Prussic Acid in Forages

    E-Print Network [OSTI]

    Provin, Tony; Pitt, John L.

    2003-01-06T23:59:59.000Z

    When nitrates and prussic acid accumulate in forage, the feed may not be safe for livestock consumption. Learn the symptoms of nitrate and prussic acid poisoning and which plants are most likely to pose a risk to livestock. Also learn sampling...

  5. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, M.M.; Shoup, T.

    1998-10-06T23:59:59.000Z

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is [{sup 18}F]-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an {alpha}-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of {alpha}-aminoisobutyric acid.

  6. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, M.M.; Shoup, T.

    1998-09-15T23:59:59.000Z

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is [{sup 18}F]-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an {alpha}-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of {alpha}-aminoisobutyric acid.

  7. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, Mark M. (Atlanta, GA); Shoup, Timothy (Decatur, GA)

    1998-09-15T23:59:59.000Z

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is ›.sup.18 F!-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an .alpha.-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of .alpha.-aminoisobutyric acid.

  8. Amino acid analogs for tumor imaging

    DOE Patents [OSTI]

    Goodman, Mark M. (Atlanta, GA); Shoup, Timothy (Decatur, GA)

    1998-10-06T23:59:59.000Z

    The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of 1-amino-cycloalkyl-1-carboxylic acids, namely, their rapid uptake and prolonged retention in tumors with the properties of halogen substituents, including certain useful halogen isotopes including fluorine-18, iodine-123, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77 and bromine-82. In one aspect, the invention features amino acid compounds that have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds show a target to non-target ratio of at least 5:1, are stable in vivo and substantially localized to target within 1 hour after administration. An especially preferred amino acid compound is ›.sup.18 F!-1-amino-3-fluorocyclobutane-1-carboxylic acid (FACBC). In another aspect, the invention features pharmaceutical compositions comprised of an .alpha.-amino acid moiety attached to either a four, five, or a six member carbon-chain ring. In addition, the invention features analogs of .alpha.-aminoisobutyric acid.

  9. Nanoparticles modified with multiple organic acids

    DOE Patents [OSTI]

    Cook, Ronald Lee (Lakewood, CO); Luebben, Silvia DeVito (Golden, CO); Myers, Andrew William (Arvada, CO); Smith, Bryan Matthew (Boulder, CO); Elliott, Brian John (Superior, CO); Kreutzer, Cory (Brighton, CO); Wilson, Carolina (Arvada, CO); Meiser, Manfred (Aurora, CO)

    2007-07-17T23:59:59.000Z

    Surface-modified nanoparticles of boehmite, and methods for preparing the same. Aluminum oxyhydroxide nanoparticles are surface modified by reaction with selected amounts of organic acids. In particular, the nanoparticle surface is modified by reactions with two or more different carboxylic acids, at least one of which is an organic carboxylic acid. The product is a surface modified boehmite nanoparticle that has an inorganic aluminum oxyhydroxide core, or part aluminum oxyhydroxide core and a surface-bonded organic shell. Organic carboxylic acids of this invention contain at least one carboxylic acid group and one carbon-hydrogen bond. One embodiment of this invention provides boehmite nanoparticles that have been surface modified with two or more acids one of which additional carries at least one reactive functional group. Another embodiment of this invention provides boehmite nanoparticles that have been surface modified with multiple acids one of which has molecular weight or average molecular weight greater than or equal to 500 Daltons. Yet, another embodiment of this invention provides boehmite nanoparticles that are surface modified with two or more acids one of which is hydrophobic in nature and has solubility in water of less than 15 by weight. The products of the methods of this invention have specific useful properties when used in mixture with liquids, as filler in solids, or as stand-alone entities.

  10. Nitrates and Prussic Acid in Forages 

    E-Print Network [OSTI]

    Provin, Tony; Pitt, John L.

    2003-01-06T23:59:59.000Z

    When nitrates and prussic acid accumulate in forage, the feed may not be safe for livestock consumption. Learn the symptoms of nitrate and prussic acid poisoning and which plants are most likely to pose a risk to livestock. Also learn sampling...

  11. Surfactant Screening to Alter the Wettability and Aid in Acidizing Carbonate Formations

    E-Print Network [OSTI]

    Yadhalli Shivaprasad, Arun Kumar

    2013-02-26T23:59:59.000Z

    , known as high temperature organic acids (HTO acids), have been found to be useful in acidizing subterranean formations at temperatures up to 400oF. Some of these acids are oxalic acid, malonic acid, pimelic acid, succinic acid, glutaric acid, adipic... acid and their mixtures. In addition to creating wormholes in carbonate formations, HTO acids can remove carbonate scale at high temperatures and cause very low corrosion to the tubing and casing. 6 1.2 Role of Surfactants in Acidizing 1...

  12. Experimental Study of Acid Fracture Conductivity of Austin Chalk Formation

    E-Print Network [OSTI]

    Nino Penaloza, Andrea

    2013-05-01T23:59:59.000Z

    to those in actual acid fracture treatments. After acid etching, fracture conductivity is measured at different closure stresses. This research work presents a systematic study to investigate the effect of temperature, rock-acid contact time and initial...

  13. acid-base imbalance: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    acid generates a material (PE-C02H Deutch, John 59 Acid-Based Synthesis of Monodisperse Rare-Earth-Doped Colloidal SiO2 Spheres Materials Science Websites Summary: Acid-Based...

  14. Experimental Study of Acid Fracture Conductivity of Austin Chalk Formation 

    E-Print Network [OSTI]

    Nino Penaloza, Andrea

    2013-05-01T23:59:59.000Z

    Acid fracture conductivity and the effect of key variables in the etching process during acid fracturing can be assessed at the laboratory scale. This is accomplished by using an experimental apparatus that simulates acid injection fluxes comparable...

  15. Investigations of amino acid-based surfactants at liquid interfaces 

    E-Print Network [OSTI]

    Yang, Dengliang

    2005-11-01T23:59:59.000Z

    Herein are presented collective studies of amino acid-based surfactants, also known as lipoamino acids, at liquid interfaces. Chapter III describes an investigation of domain morphology of N-Stearoylglutamic acid (N-SGA) ...

  16. aqueous tartaric acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    with limited success.2,3 During the last decade, attention to sulfuric acid anodizing and boric-sulfuric acid Paris-Sud XI, Universit de 255 Si isotope systematics of acidic...

  17. Difunctional carboxylic acid anions in oilfield waters

    SciTech Connect (OSTI)

    MacGowan, D.B.; Surdam, R.C.

    1988-01-01T23:59:59.000Z

    Recent models of porosity enhancement during sandstone diagenesis have called upon the metal complexing ability of difunctional carboxylic acid anions in subsurface waters to explain aluminosilicate dissolution. Although carboxylic acid anions have been known to exist in oilfield waters since the turn of the century, until now the existence of significant concentrations of difunctional carboxylic acid anions has not been documented. Data from this study show that difunctional carboxylic acid anions can exist in concentrations up to 2640 ppm, and can account for nearly 100% of the organic acid anions in some oilfield waters. Formation water samples with exceptionally high concentrations of difunctional carboxylic acid anions are found in reservoirs which are at maximum levels of thermal exposure, and which are presently in the 80-100/sup 0/C thermal window. Plagioclase dissolution experiments performed with natural oilfield waters and artificial solutions indicate that waters with high difunctional acid anion concentrations are capable, by organo-metallic complexation, of being apparently oversaturated with respect to total aluminum concentrations compared to the inorganic solubility of kaolinite by several orders of magnitude. Dissolution experiments simulating a specific geologic environment (Stevens Sandstone, southern San Joaquin Basin, California; using natural oilfield waters and Stevens Sandstone core samples), produced plagioclase and calcite dissolution textures similar to those noted in well cores from the Stevens Sandstone, as well as raising total aluminum concentrations in these experimental solutions several orders of magnitude over the solubility of kaolinite.

  18. Production of Succinic Acid for Lignocellulosic Hydrolysates

    SciTech Connect (OSTI)

    Davison, B.H.; Nghiem, J.

    2002-06-01T23:59:59.000Z

    The purpose of this Cooperative Research and Development Agreement (CRADA) is to add and test new metabolic activities to existing microbial catalysts for the production of succinic acid from renewables. In particular, they seek to add to the existing organism the ability to utilize xylose efficiently and simultaneously with glucose in mixtures of sugars or to add succinic acid production to another strain and to test the value of this new capability for production of succinic acid from industrial lignocellulosic hydrolyasates. The Contractors and Participant are hereinafter jointly referred to as the 'Parties'. Research to date in succinic acid fermentation, separation and genetic engineering has resulted in a potentially economical process based on the use of an Escherichia coli strain AFP111 with suitable characteristics for the production of succinic acid from glucose. Economic analysis has shown that higher value commodity chemicals can be economically produced from succinic acid based on repliminary laboratory findings and predicted catalytic parameters. The initial target markets include succinic acid itself, succinate salts, esters and other derivatives for use as deicers, solvents and acidulants. The other commodity products from the succinic acid platform include 1,4-butanediol, {gamma}-butyrolactone, 2-pyrrolidinone and N-methyl pyrrolidinone. Current economic analyses indicate that this platform is competitive with existing petrochemical routes, especially for the succinic acid and derivatives. The report presents the planned CRADA objectives followed by the results. The results section has a combined biocatalysis and fermentation section and a commercialization section. This is a nonproprietary report; additional proprietary information may be made available subject to acceptance of the appropriate proprietary information agreements.

  19. Relative reactivities of solid benzoic acids

    E-Print Network [OSTI]

    Warwas, Edwin James

    1967-01-01T23:59:59.000Z

    REACTIVITIES OF SOLID BENZOIC ACIDS (January 1967) Edwin James Warwas B. S. , Southwest Texas State College Directed by: Dr. C. K. Hancock and Dr. E. A. Meyers The reactions of solid benzoic acid (BZAH) and nine m- or p- substituted benzoic acids (RBZAH...) with solid potassium benzoate (BZAK) and m- or p-substituted potassium benzoates (R'BZAK) have been carried out in sealed thin-walled glass capillary tubes or in 0 sealed weighing bottles at 70 For the reaction, RBZAH + R'BZAK, where R = R', the product...

  20. aspartic acid racemization: Topics by E-print Network

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    rate of racemization for amino acids preserved in planktonic foraminifera climate change. Keywords: amino acid racemization, Quaternary geochronology, Arctic Ocean, planktonic...

  1. Catalytic Consequences of Acid Strength in the Conversion of...

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    Consequences of Acid Strength in the Conversion of Methanol to Dimethyl Ether. Catalytic Consequences of Acid Strength in the Conversion of Methanol to Dimethyl Ether. Abstract:...

  2. acid bacteria revisited: Topics by E-print Network

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    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  3. acid bacteria elicit: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  4. acid bacteria affect: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  5. acid bacteria structure: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  6. acid bacteria inducing: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  7. acid bacteria purification: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Summary: The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Clark of amino acid in soil using radioactive isotopes, however many experiments...

  8. acid bacteria enhance: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    de 4 The Fate of Amino Acid in Soil Experiments: Bacteria, Roots and Fungi Melissa Campbell Environmental Sciences and Ecology Websites Summary: The Fate of Amino Acid in...

  9. acid conserved neutralizing: Topics by E-print Network

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    Websites Summary: itself against acidification and is used to monitor the effect of acid rain on watersheds. From 1993MASTERS REPORT ANALYSIS AND MODELING OF ACID...

  10. acid biosynthesis revealed: Topics by E-print Network

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    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  11. acid biosynthesis inhibitors: Topics by E-print Network

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    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  12. abscisic acid biosynthesis: Topics by E-print Network

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    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  13. acid neutralization capacity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: itself against acidification and is used to monitor the effect of acid rain on watersheds. From 1993MASTERS REPORT ANALYSIS AND MODELING OF ACID...

  14. acid neutralizing capacity: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: itself against acidification and is used to monitor the effect of acid rain on watersheds. From 1993MASTERS REPORT ANALYSIS AND MODELING OF ACID...

  15. acid levels metabolic: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  16. acid function biosynthesis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  17. acid biosynthesis leads: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  18. acid soil tolerance: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Websites Summary: 1980; however, soils collected during the earlier phases of acid rain research have led to a growing Indications of Soil Recovery from Acidic...

  19. acid biosynthesis synthesis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  20. acid biosynthesis genes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Websites Summary: bioavailability of aluminum triggered by in- dustrialization and acid rain 20. The presence of organic acidsThe Metabolism of Aluminum Citrate and...

  1. Hydrogen-bond acidic functionalized carbon nanotubes (CNTs) with...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Hydrogen-bond acidic functionalized carbon nanotubes (CNTs) with covalently-bound hexafluoroisopropanol groups. Hydrogen-bond acidic functionalized carbon nanotubes (CNTs) with...

  2. Effects of Continuous Triiodothyronine Infusion on Citric Acid...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Continuous Triiodothyronine Infusion on Citric Acid Cycle in the Normal Immature Swine Heart under Extracorporeal Effects of Continuous Triiodothyronine Infusion on Citric Acid...

  3. Copper isotope fractionation in acid mine drainage. | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in acid mine drainage. Abstract: We surveyed the Cu isotopic composition of primary minerals and stream water affected by acid mine drainage in a mineralized watershed located in...

  4. AVTA: 2010 Honda Civic HEV with Experimental Ultra Lead Acid...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    0 Honda Civic HEV with Experimental Ultra Lead Acid Battery Testing Results AVTA: 2010 Honda Civic HEV with Experimental Ultra Lead Acid Battery Testing Results The Vehicle...

  5. Acid Initiation of Ammonia-Borane Dehydrogenation for Hydrogen...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Initiation of Ammonia-Borane Dehydrogenation for Hydrogen Storage. Acid Initiation of Ammonia-Borane Dehydrogenation for Hydrogen Storage. Abstract: An abstract for this...

  6. Electrodeposition From Acidic Solutions of Nickel Bis(benzenedithiolat...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    From Acidic Solutions of Nickel Bis(benzenedithiolate) Produces a Hydrogen-Evolving Ni-S Film on Glassy Carbon Electrodeposition From Acidic Solutions of Nickel...

  7. Lewis Acid-Base Interactions between Polysulfides and Metal Organic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Lewis Acid-Base Interactions between Polysulfides and Metal Organic Framework in Lithium Sulfur Batteries. Lewis Acid-Base Interactions between Polysulfides and Metal Organic...

  8. amino acid pattern: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Sequence Analysis Pradipta Maji and Sankar K. Pal, Fellow, IEEE Abstract--In most pattern recognition algorithms, amino acids feature space. It is designed using an amino...

  9. acid exhibits selective: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Sequence Analysis Pradipta Maji and Sankar K. Pal, Fellow, IEEE Abstract--In most pattern recognition algorithms, amino acids feature space. It is designed using an amino...

  10. amino acid selective: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Sequence Analysis Pradipta Maji and Sankar K. Pal, Fellow, IEEE Abstract--In most pattern recognition algorithms, amino acids feature space. It is designed using an amino...

  11. amino acid selection: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Sequence Analysis Pradipta Maji and Sankar K. Pal, Fellow, IEEE Abstract--In most pattern recognition algorithms, amino acids feature space. It is designed using an amino...

  12. amino acid mutations: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Sequence Analysis Pradipta Maji and Sankar K. Pal, Fellow, IEEE Abstract--In most pattern recognition algorithms, amino acids feature space. It is designed using an amino...

  13. amino acid recognition: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Acid Sequence Analysis Pradipta Maji and Sankar K. Pal, Fellow, IEEE Abstract--In most pattern recognition algorithms, amino acids feature space. It is designed using an amino...

  14. amino acid insertion: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    their distribution in known structures with experimental data such as amino acid transfer free energy scales (water to membrane center and water Senes, Alessandro 2 Amino Acid...

  15. Selective Removal of Lanthanides from Natural Waters, Acidic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Removal of Lanthanides from Natural Waters, Acidic Streams and Dialysate. Selective Removal of Lanthanides from Natural Waters, Acidic Streams and Dialysate. Abstract: The...

  16. acid increases expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that are especially sensitive to increasing nutrient Gotelli, Nicholas J. 378 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  17. ascorbic acid: Topics by E-print Network

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    are stable thermodynamically and hydration free energies obtained Yu, Fangqun 78 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  18. acid isopropyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 192 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  19. acid vinyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 280 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  20. ascorbic acid phenolic: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 229 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  1. acid inhibits production: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sciences and Ecology Websites Summary: Animals excrete three main nitrogen products, ammonia, urea and uric acid (Fig. 1), as well as some and amino acids. The term ammonia...

  2. acid monoethyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 180 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  3. antioxidant ascorbic acid: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 218 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  4. acid copolymeric hydrogels: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 278 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  5. acid solutions part: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    m Popovych, Roman 71 Z .Comparative Biochemistry and Physiology Part B 128 2001 445 450 Purification and characterization of the fatty acid Chemistry Websites Summary: fatty acid...

  6. acid hydrolysis: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 213 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  7. acid fume system: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 147 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  8. acid synthase type: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 328 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  9. ascorbic acid induces: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ramsey Theory Continue with Nesetril-Rodl Reminder Prigozhin, Leonid 174 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  10. ascorbic acid fuel: Topics by E-print Network

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    stable thermodynamically and hydration free energies obtained Yu, Fangqun 314 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  11. acid ester prodrugs: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 194 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  12. acid allyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 239 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  13. ascorbic acid determination: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 188 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  14. ascorbic acid influence: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Golnari, Golshan; Banerjee, Arindam; Zhang, Zhi-Li 2014-01-01 176 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  15. ascorbic acid sodium: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 338 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  16. acid phenylethyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 180 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  17. acrylic acid esters: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 242 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  18. acid dimethyl ester: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 227 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  19. acid decarboxylase expression: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the expressions. Anna Sthl; Petra Sundstrm; Kristina Hk 2005-01-01 180 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...

  20. acid phosphatase 5a: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    stable thermodynamically and hydration free energies obtained Yu, Fangqun 193 ANTIBODY PURIFICATION USING CAPRYLIC ACID In mildly acidic conditions, the addition of short-chain...