National Library of Energy BETA

Sample records for non-acid elution nae

  1. EVALUATION OF POTENTIAL ELUANTS FOR NON-ACID ELUTION OF CESIUM FROM SPHERICAL RESORCINOL-FORMALDEHYDE RESIN

    SciTech Connect (OSTI)

    Adu-Wusu, K.; Nash, C.; Pennebaker, F.

    2011-10-23

    Ion Exchange column loading and elution of cesium from spherical resorcinol-formaldehyde resin have been conducted for two potential non-acid eluants -(NH{sub 4}){sub 2}CO{sub 3} and CH{sub 3}COONH{sub 4}. The results revealed encouraging cesium elution performance. 100% elution was achieved in at most 22 hours ({approx}28 bed volumes) of elution. Elution performance was fairly high at 6 hours ({approx}8 bed volumes) of elution for some of the eluants and also practically comparable to the benchmark acid eluant (HNO{sub 3}). Hence, it is quite possible 100% percent elution will be closer to the 6th hour than the 22nd hour. Elution is generally enhanced by increasing the concentration and pH of the eluants, and combining the eluants.

  2. Los Alamos engineer selected to participate in NAE's 2012 "Frontiers...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Moody to participate in "Frontiers of Engineering" Los Alamos engineer selected to participate in NAE's 2012 "Frontiers of Engineering" symposium Engineers between 30 to 45 who are...

  3. CX-008371: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Non-Acid Elution (NAE) of Cesium From Resorcinol-Formaldehyde Resin CX(s) Applied: B3.6 Date: 03/27/2012 Location(s): South Carolina Offices(s): Savannah River Operations Office

  4. CX-005500: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Non-Acid Elution (NAE) of Cesium From Resorcinol-Formaldehyde ResinCX(s) Applied: B3.6Date: 02/24/2011Location(s): Aiken, South CarolinaOffice(s): Environmental Management, Savannah River Operations Office

  5. Danny D. Reible, PhD PE BCEE NAE Donovan Maddox Distinguished Engineering Chair

    E-Print Network [OSTI]

    Chen, Xinzhong

    Union, American Society for Engineering Education, Association of Environmental Engineering and ScienceDanny D. Reible, PhD PE BCEE NAE Donovan Maddox Distinguished Engineering Chair Environmental and Water Resources, Civil and Environmental Engineering, Texas Tech University Ph: 806-742-3523 Fax: 806

  6. Douglas M. Chapin, Ph.D., NAE, Fellow, American Nuclear Society is a Principal of MPR Associates, Inc. He holds a B.S. in Electrical

    E-Print Network [OSTI]

    Aydilek, Ahmet

    Douglas M. Chapin, Ph.D., NAE, Fellow, American Nuclear Society is a Principal of MPR Associates.D. in Nuclear Studies in Chemical Engineering and Nuclear Engineering from Princeton University. Dr. Chapin has worked in the nuclear industry since 1962, beginning with four years in the Naval Reactors design group

  7. Innovative Elution Processes for Recovering Uranium from Seawater

    SciTech Connect (OSTI)

    Wai, Chien; Tian, Guoxin; Janke, Christopher

    2014-05-29

    Utilizing amidoxime-based polymer sorbents for extraction of uranium from seawater has attracted considerable interest in recent years. Uranium collected in the sorbent is recovered typically by elution with an acid. One drawback of acid elution is deterioration of the sorbent which is a significant factor that limits the economic competitiveness of the amidoxime-based sorbent systems for sequestering uranium from seawater. Developing innovative elution processes to improve efficiency and to minimize loss of sorbent capacity become essential in order to make this technology economically feasible for large-scale industrial applications. This project has evaluated several elution processes including acid elution, carbonate elution, and supercritical fluid elution for recovering uranium from amidoxime-based polymer sorbents. The elution efficiency, durability and sorbent regeneration for repeated uranium adsorption- desorption cycles in simulated seawater have been studied. Spectroscopic techniques are used to evaluate chemical nature of the sorbent before and after elution. A sodium carbonate-hydrogen peroxide elution process for effective removal of uranium from amidoxime-based sorbent is developed. The cause of this sodium carbonate and hydrogen peroxide synergistic leaching of uranium from amidoxime-based sorbent is attributed to the formation of an extremely stable uranyl peroxo-carbonato complex. The efficiency of uranium elution by the carbonate-hydrogen peroxide method is comparable to that of the hydrochloric acid elution but damage to the sorbent material is much less for the former. The carbonate- hydrogen peroxide elution also does not need any elaborate step to regenerate the sorbent as those required for hydrochloric acid leaching. Several CO2-soluble ligands have been tested for extraction of uranium from the sorbent in supercritical fluid carbon dioxide. A mixture of hexafluoroacetylacetone and tri-n-butylphosphate shows the best result but uranium removal from the sorbent reaches only 80% after 10 hours of leaching. Some information regarding coordination of vanadium with amidoxime molecules and elution of vanadium from amidoxime- based sorbents is also given in the report.

  8. Cleanup Handbook For RNA cleanup and concentration with small elution

    E-Print Network [OSTI]

    Kirschner, Marc W.

    information for your local QIAGEN distributor. Australia QIAGEN Pty Ltd PO Box 25 · Clifton Hill · Victoria Worldwide #12;RNeasy MinElute Cleanup Handbook 03/2003 3 Contents Kit Contents 4 Storage 4 Product Use Limitations 4 Product Warranty and Satisfaction Guarantee 5 Quality Control 5 Technical Assistance 5 Safety

  9. ORNL/TM-2008/194 Alternate Methods for Eluting Cesium from

    E-Print Network [OSTI]

    Pennycook, Steve

    ORNL/TM-2008/194 Alternate Methods for Eluting Cesium from Spherical Resorcinol-Formaldehyde Resin and Technology Division ALTERNATE METHODS FOR ELUTING CESIUM FROM SPHERICAL RESORCINOL-FORMALDEHYDE RESIN P. A................................................................................................7 3.1 Cesium Loading

  10. DRUG DELIVERY IN BIOLOGICAL TISSUES: AN APPLICATION TO THE ELUTING STENT

    E-Print Network [OSTI]

    Pontrelli, Giuseppe

    DRUG DELIVERY IN BIOLOGICAL TISSUES: AN APPLICATION TO THE ELUTING STENT G. Pontrelli1 , F. de Monte2 , M. Prosi3 1. ABSTRACT The drug diffusion process through an arterial eluting stent is studied and a quantitative description for drug transport to evaluate feasibility of new drug delivery strategies

  11. Mathematical modeling and simulation of intravascular drug delivery from drug-eluting stents with biodegradable PLGA coating

    E-Print Network [OSTI]

    Zhu, Xiaoxiang, Ph. D. Massachusetts Institute of Technology

    2014-01-01

    Drug-eluting stents (DES) are commonly used in coronary angioplasty procedures. A DES elutes drug compounds from a thin polymeric coating into the surrounding coronary artery tissue to reduce in-stent restenosis (a significant ...

  12. Small-Column Cesium Ion Exchange Elution Testing of Spherical Resorcinol-Formaldehyde

    SciTech Connect (OSTI)

    Brown, Garrett N.; Russell, Renee L.; Peterson, Reid A.

    2011-10-21

    This report summarizes the work performed to evaluate multiple, cesium loading, and elution cycles for small columns containing SRF resin using a simple, high-level waste (HLW) simulant. Cesium ion exchange loading and elution curves were generated for a nominal 5 M Na, 2.4E-05 M Cs, 0.115 M Al loading solution traced with 134Cs followed by elution with variable HNO3 (0.02, 0.07, 0.15, 0.23, and 0.28 M) containing variable CsNO3 (5.0E-09, 5.0E-08, and 5.0E-07 M) and traced with 137Cs. The ion exchange system consisted of a pump, tubing, process solutions, and a single, small ({approx}15.7 mL) bed of SRF resin with a water-jacketed column for temperature-control. The columns were loaded with approximately 250 bed volumes (BVs) of feed solution at 45 C and at 1.5 to 12 BV per hour (0.15 to 1.2 cm/min). The columns were then eluted with 29+ BVs of HNO3 processed at 25 C and at 1.4 BV/h. The two independent tracers allowed analysis of the on-column cesium interaction between the loading and elution solutions. The objective of these tests was to improve the correlation between the spent resin cesium content and cesium leached out of the resin in subsequent loading cycles (cesium leakage) to help establish acid strength and purity requirements.

  13. Everolimus-induced Pneumonitis after Drug-eluting Stent Implantation: A Case Report

    SciTech Connect (OSTI)

    Sakamoto, Susumu Kikuchi, Naoshi; Ichikawa, Atsuo; Sano, Go; Satoh, Keita; Sugino, Keishi; Isobe, Kazutoshi; Takai, Yujiro; Shibuya, Kazutoshi; Homma, Sakae

    2013-08-01

    Despite the wide use of everolimus as an antineoplastic coating agent for coronary stents to reduce the rate of restenosis, little is known about the health hazards of everolimus-eluting stents (EES). We describe a case of pneumonitis that developed 2 months after EES implantation for angina. Lung pathology demonstrated an organizing pneumonia pattern that responded to corticosteroid therapy. Although the efficacy of EES for ischemic heart disease is well established, EES carries a risk of pneumonitis.

  14. Bradycardia Associated With Drug-Eluting Beads Loaded With Irinotecan (DEBIRI) Infusion for Colorectal Liver Metastases

    SciTech Connect (OSTI)

    Pua, Uei

    2013-06-15

    Intra-arterial injection of drug-eluting beads loaded with irinotecan (DEBIRI) is a new treatment option being investigated, with encouraging results, for unresectable colorectal liver metastases that are refractory to systemic chemotherapy (Martin et al., Ann Surg Oncol 18:192-198, 2011). Toxicity related to DEBIRI has also been described (Martin et al., Cardiovasc Intervent Radiol 33:960-966, 2010). Nevertheless, experience and literature related to DEBIRI remain limited, and experience with this treatment is expected to increase. The purpose of this article is to describe bradycardia occurring during DEBIRI administration, which has not been reported thus far.

  15. NAE National Meeting Thursday February 7, 2013

    E-Print Network [OSTI]

    Zhou, Chongwu

    #12;» Make Solar Energy Economical " » Provide Energy from Fusion" » Develop Carbon Sequestration.Internet" 14.Imaging" 15.Household Appliances" 16.Health Technologies" 17.Petroleum and Petrochemical"? (Plato)" > Most are Transformative: " » Energy from Fusion" » Reverse Engineering the Brain" > All

  16. Irinotecan Loaded in Eluting Beads: Preclinical Assessment in a Rabbit VX2 Liver Tumor Model

    SciTech Connect (OSTI)

    Rao, Pramod P.; Pascale, Florentina [Institute Gustave Roussy, Department of Interventional Radiology (France); Seck, Atman [Institute Gustave Roussy, UPRES EA 3535, Pharmacologie et Nouveaux Traitements du Cancer (France); Auperin, Anne [Institute Gustave Roussy, Department of Biostatistics and Epidemiology (France); Drouard-Troalen, Laurence [Institute Gustave Roussy, Department of Biology and Pathology (France); Deschamps, Frederic; Teriitheau, Christophe [Institute Gustave Roussy, Department of Interventional Radiology (France); Paci, Angelo [Institute Gustave Roussy, UPRES EA 3535, Pharmacologie et Nouveaux Traitements du Cancer (France); Denys, Alban; Bize, Pierre [Centre Hospitalier Universitaire Vaudois, Department of Interventional Radiology (Switzerland); Baere, Thierry de, E-mail: debaere@igr.fr [Institute Gustave Roussy, Department of Interventional Radiology (France)

    2012-12-15

    Purpose: The purpose of this study was to study the pharmacokinetics of irinotecan injected intravenously, intra-arterially, or loaded onto a delivery platform. Material and Methods: Fifty-four New Zealand White rabbits with VX2 liver tumor, divided in 3 groups of 17 rabbits, each received irinotecan either by intravenous (IV) route, intra-arterial hepatic (IA) route, or loaded on drug-eluting beads (DEBIRI). Animals were killed at 1, 6, and 24 h. Irinotecan and SN-38 concentrations were measured at different time points in serum, tumor, and normal liver.ResultsTwelve milligrams of irinotecan were injected IV and IA, whereas 6-16.5 mg were injected loaded onto DEBIRI. Normalized serum irinotecan reached a peak of 333 ng/ml (range 198.8-502.5) for IV, 327.1 ng/ml (range 277.1-495.6) for IA, and 189.7 ng/ml (range 111.1-261.9) for DEBIRI (P < 0.001) delivery. The area-under-the-curve value from 10 to 60 min of serum irinotecan concentration was significantly lower for DEBIRI (P = 0.0009). Tumor irinotecan levels for IV, IA, and DEBIRI (in ng/200 mg of tissue followed by ranges in parentheses) were, respectively, 23.6 (0.3-24.9), 36.5 (7.7-1914.1), and 20.2 (2.9-319) at 1 h; 4.2 (1-27.9), 99.3 (46.6-159.5), and 42.1 (11.3-189) at 6 h; and 2.7 (2.5-6.9), 18.3 (1.5-369.1), and 174.4 (3.4-5147.3) at 24 h (P = 0.02). At 24 h, tumor necrosis was 25% (10-30), 60% (40-91.25), and 95% (76.25-95) for IV, IA, and DEBIRI, respectively (P = 0.03). Conclusion: Compared with IV or IA, DEBIRI induces lower early serum levels of irinotecan, a high and prolonged intratumoral level of irinotecan, and a greater rate of tumor necrosis at 24 h. Further evaluation of the clinical benefit of DEBIRI is warranted.

  17. ven as the National Academy of Engineering (NAE) announced its

    E-Print Network [OSTI]

    the health and well being of the planet and quality of life. Challenges range from making solar energy affordable to engi- neering better medicines, and from improving urban infrastructure to secur- ing cyber space. "Vanderbilt Engineering faculty members are actively conducting research in many of these areas

  18. NAS/NAE Committee on the Prospects for IFE Systems

    E-Print Network [OSTI]

    for Polar-Drive Ignition on the NIF J. D. Zuegel University of Rochester Laboratory for Laser Energetics 0 0 be tested on the NIF with a few modest modifications to the facility · Beam-smoothingimprovements: ­ Multi modifications to the NIF facility ­ Beamsmoothingisonlyrequiredatthebeginningofthelaserpulse, which minimizes

  19. Drug-Eluting Nitinol Stent Treatment of the Superficial Femoral Artery and Above-the-Knee Popliteal Artery (The Zilver PTX Single-Arm Clinical Study): A Comparison Between Diabetic and Nondiabetic Patients

    SciTech Connect (OSTI)

    Fanelli, Fabrizio, E-mail: fabrizio.fanelli@uniroma1.it [Sapienza University of Rome, Vascular and Interventional Radiology Unit, Department of Radiological Sciences (Italy)] [Sapienza University of Rome, Vascular and Interventional Radiology Unit, Department of Radiological Sciences (Italy); Primo, Massimiliano Di [Hopital Europeen Georges Pompidou, University Paris Descartes (France)] [Hopital Europeen Georges Pompidou, University Paris Descartes (France); Boatta, Emanuele [Sapienza University of Rome, Vascular and Interventional Radiology Unit, Department of Radiological Sciences (Italy)] [Sapienza University of Rome, Vascular and Interventional Radiology Unit, Department of Radiological Sciences (Italy); Johnston, Krystal, E-mail: kjohnston@medinst.com [MED Institute, Inc (United States)] [MED Institute, Inc (United States); Sapoval, Marc, E-mail: marc.sapoval2@egp.aphp.fr [Hopital Europeen Georges Pompidou, University Paris Descartes (France)] [Hopital Europeen Georges Pompidou, University Paris Descartes (France)

    2013-10-15

    Purpose: To describe the 1-year results of drug-eluting nitinol stent placement in the femoropopliteal artery of diabetic and nondiabetic patients. Materials and Methods: All patients enrolled in this prospective, multicenter study underwent paclitaxel-eluting stent placement for de novo or restenotic lesions of the superficial femoral and/or popliteal artery. Baseline and follow-up walking impairment questionnaire (WIQ) scores, Rutherford classifications, and ankle-brachial index (ABI) measurements were obtained. Follow-up was completed at 1, 6, and 12 months. Results: There were 285 diabetic patients and 502 nondiabetic patients treated. There were no significant differences in mean lesion length or lesion calcification between patient groups. Procedural success in both treatment groups was >97 %. There were no significant differences between diabetic and nondiabetic groups in Kaplan-Meier estimates of patency, event-free survival (EFS), or freedom from target lesion revascularization (TLR) at 6 and 12 months. Both groups experienced a significant increase in ABI and WIQ values after treatment, and these improvements were sustained to 12-month follow-up; however, nondiabetic patients had significantly greater 6- and 12-month WIQ scores compared with diabetic patients. Based on covariate analysis, the only factors shown to be significant and to negatively influence patency were longer lesion length (p = 0.009), higher Rutherford classification (p = 0.02), and lack of hypertension (p = 0.02); diabetic status was not found to be a significant factor. Conclusion: Diabetic and nondiabetic patients had similar estimates of primary patency, EFS, and freedom from TLR; however, diabetic patients showed less improvement in WIQ scores compared with nondiabetic patients.

  20. The Interface between P and NP: COL, XOR, NAE, 1-in-, and Horn SAT

    E-Print Network [OSTI]

    Walsh, Toby

    question. We identify phase transition behavior in each of these problem classes. Surprisingly we observe makes NP-complete problems hard to solve? Re- search into phase transition behavior has given much at §¨¦! " (Mitchell, Selman, & Levesque 1992). Associated with this transition is a rapid increase in problem

  1. NAS-NAE National Convocation on "Rising Above the Gathering Storm...

    Energy Savers [EERE]

    I don't have to remind you of the challenges we face: rapidly growing global demand for energy, along with rising prices. And these demand pressures will only increase with time....

  2. NAS-NAE National Convocation on "Rising Above the Gathering Storm Two Years

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergy AEnergy Managing SwimmingMicrosoft Word1Sustainability inDeputyNAICSLater:

  3. Los Alamos engineer selected to participate in NAE's 2012 "Frontiers of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCentermillion to local Unitedto STEMLANL conductsLos

  4. The Interface between P and NP: COL, XOR, NAE, 1ink, and Horn SAT Cork Constraint Computation Center

    E-Print Network [OSTI]

    Walsh, Toby

    question. We identify phase transition behavior in each of these problem classes. Surprisingly we observe makes NP­complete problems hard to solve? Re­ search into phase transition behavior has given much at l=n #25; 4:3 (Mitchell, Selman, & Levesque 1992). Associated with this transition is a rapid

  5. Spontaneous Liver Rupture After Treatment With Drug-Eluting Beads

    SciTech Connect (OSTI)

    Ritter, C. O., E-mail: ritter@roentgen.uni-wuerzburg.de [University of Wuerzburg, Institute of Radiology (Germany); Wartenberg, M.; Mottok, A. [University of Wuerzburg, Institute of Pathology (Germany); Steger, U. [University of Wuerzburg, Department of General, Visceral, Vascular, and Pediatric Surgery (Germany); Goltz, J. P.; Hahn, D.; Kickuth, R. [University of Wuerzburg, Institute of Radiology (Germany)

    2012-02-15

    Spontaneous rupture of hepatocellular carcinoma (HCC) after transcatheter arterial chemoembolization (TACE) is a rare and life-threatening complication. Pathophysiologic mechanisms are not yet fully known; it is suggested that rupture is preceded by reactive tissue edema and intratumerous bleeding, leading to a rapid expansion of tumour mass with risk of extrahepatic bleeding in the case of subcapsular localisation. This case report discusses a sudden, unexpected lethal complication in a 74 year-old male patient treated with TACE using DC Bead loaded with doxorubicin (DEBDOX) in a progressive multifocal HCC.

  6. ELUTIONS Inc formerly TeCom | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTIONRobertsdale, Alabama (UtilityInstrumentsAreaforInformation ECr TechnologiesEERE -

  7. Engine Design for LIFE Presentation to

    E-Print Network [OSTI]

    be transported for maintenance or replacement Unsealed chamber, separate from the vacuum and optical systems connections are made with hydraulic couplers from industry Latkowski -- NAS/NAE, January 2011Latkowski -- NAS with hydraulic couplers from industry Latkowski -- NAS/NAE, January 2011Latkowski -- NAS/NAE, January 2011NIF

  8. Chemoembolization With Doxorubicin-Eluting Beads for Unresectable Hepatocellular Carcinoma: Five-Year Survival Analysis

    SciTech Connect (OSTI)

    Malagari, Katerina; Pomoni, Mary; Moschouris, Hippocrates; Bouma, Evanthia; Koskinas, John; Stefaniotou, Aspasia; Marinis, Athanasios; Kelekis, Alexios; Alexopoulou, Efthymia; Chatziioannou, Achilles; Chatzimichael, Katerina; Dourakis, Spyridon; Kelekis, Nikolaos; Rizos, Spyros; Kelekis, Dimitrios

    2012-10-15

    Purpose: The purpose of this study was to report on the 5-year survival of hepatocellular carcinoma (HCC) patients treated with DC Bead loaded with doxorubicin (DEB-DOX) in a scheduled scheme in up to three treatments and thereafter on demand. Materials and Methods: 173 HCC patients not suitable for curable treatments were prospectively enrolled (mean age 70.4 {+-} 7.4 years). Child-Pugh (Child) class was A/B (102/71 [59/41 %]), Okuda stage was 0/1/2 (91/61/19 [53.2/35.7/11.1 %]), and mean lesion diameter was 7.6 {+-} 2.1 cm. Lesion morphology was one dominant {<=}5 cm (22 %), one dominant >5 cm (41.6 %), multifocal {<=}5 (26 %), and multifocal >5 (10.4 %). Results: Overall survival at 1, 2, 3, 4, and 5 years was 93.6, 83.8, 62, 41.04, and 22.5 %, with higher rates achieved in Child class A compared with Child class B patients (95, 88.2, 61.7, 45, and 29.4 % vs. 91.5, 75, 50.7, 35.2, and 12.8 %). Mean overall survival was 43.8 months (range 1.2-64.8). Cumulative survival was better for Child class A compared with Child class B patients (p = 0.029). For patients with dominant lesions {<=}5 cm 1-, 2-, 3-, 4-, and 5-year survival rates were 100, 95.2, 71.4, 66.6, and 47.6 % for Child class A and 94.1, 88.2, 58.8, 41.2, 29.4, and 23.5 % for Child class B patients. Regarding DEB-DOX treatment, multivariate analysis identified number of lesions (p = 0.033), lesion vascularity (p < 0.0001), initially achieved complete response (p < 0.0001), and objective response (p = 0.046) as significant and independent determinants of 5-year survival. Conclusion: DEB-DOX results, with high rates of 5-year survival for patients, not amenable to curative treatments. Number of lesions, lesion vascularity, and local response were significant independent determinants of 5-year survival.

  9. USCViterbi//Engineer HTE@USC and THE BiOMEdiCal FUTURE // THE naE CHallEnGE OF SECURinG CyBERSPaCE

    E-Print Network [OSTI]

    Zhou, Chongwu

    combustion engines. This is just one of many ground- breaking research projects underway at the USC Viterbi 13 Africa, Into Grammatical English 15 Pipeline 40

  10. The Role of Neutron Activation Analysis in the Pathological Evaluation of Silver-Eluting Biomedical Devices in Biological Matrices 

    E-Print Network [OSTI]

    Lancon, Trevor

    2014-08-14

    experimental data. This system was simplified after showing that production of induced ^(109)Ag during irradiation of silver did not significantly affect elemental silver estimation by measurement of ^(110m)Ag. Results from this model suggested that samples...

  11. Local mass non-equilibrium dynamics in multi-layered porous media: application to the drug-eluting stent

    E-Print Network [OSTI]

    Pontrelli, Giuseppe

    the drug is initially loaded in polymer-encapsu- lated solid-phase, and then released both to the coating problem. Drug concentration levels and mass profiles in each layer at various times are computed, either with experimental methods [3] and with numerical simulations [4­6]. Nonetheless, many questions

  12. Food-entrained circadian rhythms are sustained in arrhythmic Clk/Clk mutant mice

    E-Print Network [OSTI]

    Silver, Rae

    Food-entrained circadian rhythms are sustained in arrhythmic Clk/Clk mutant mice SiNae Pitts,1 in final form 19 March 2003 Pitts, SiNae, Elizabeth Perone, and Rae Silver. Food- entrained circadian is a potent time cue that elicits anticipatory activity in rodents. This food-anticipatory activity (FAA

  13. BIMA Memoranda Series #22 Mapping BIMA Data with MIRIAD: A Cookbook

    E-Print Network [OSTI]

    maps from BIMA data using the MIRIAD data reduction package. Different methods are given representing the amplitude of the visibility. The anten­ nae that constitute the current baseline are labeled

  14. Apex Peptide Elution Chain Selection: A New Strategy for Selecting Precursors in 2D-LC-MALDI-TOF/TOF Experiments on Complex

    E-Print Network [OSTI]

    Breitling, Rainer

    of peptides as a conventional data-dependent acquisition method but with a 35% smaller work load. Consequently (APECS) is a simple and powerful method for intensity-based peptide selection in a complex sample profiles that link the precursor ions of the same peptide across SCX fractions. Subsequently, the precursor

  15. 1.1 0.2 were bound to the column and were eluted by 30 mM and 1 M HCI, respec-

    E-Print Network [OSTI]

    Boyer, Edmond

    infusion on postabsorp- tive glycemia in non insulin dependent diabetes mellitus (NIDDM). V Rigalleau previously reported a hyper- glycemic effect of a lipid infusion in the postabsorptive state in NIDD patients patients. Fifteen received a 180 min lipid infusion (')veiip20%'; 0.0155 mUkg/min) and 15 received saline

  16. Accounting for the mortality benefit of drug-eluting stents in percutaneous coronary intervention: a comparison of methods in a retrospective cohort study

    E-Print Network [OSTI]

    Yeh, Robert W; Chandra, Malini; McCulloch, Charles E; Go, Alan S

    2011-01-01

    this article as: Yeh et al. : Accounting for the mortalityARTICLE Open Access Accounting for the mortality benefit ofin the DES era. After accounting for these and other secular

  17. Accounting for the mortality benefit of drug-eluting stents in percutaneous coronary intervention: a comparison of methods in a retrospective cohort study

    E-Print Network [OSTI]

    Yeh, Robert W; Chandra, Malini; McCulloch, Charles E; Go, Alan S

    2011-01-01

    Waksman R: Impact of “off-label” utilization of drug-elutingstents for patients with off- and on-label indications. J Am

  18. Composition, sources, and formation of secondary organic aerosols from urban emissions

    E-Print Network [OSTI]

    Liu, Shang; Liu, Shang

    2012-01-01

    carboxylic acid, hydroxyl, and amine groups were associatedalkane, carboxylic acid, hydroxyl, amine, non-acid carbonyl,a) Alkane Carboxylic Acid Hydroxyl Amine Non?Acid Carbonyl

  19. Molecular Organic Geochemical Records of Late Ordovician Biospheric Evolution

    E-Print Network [OSTI]

    Rohrssen, Megan

    2013-01-01

    methanol (3:1 v/v) to elute saturated hydrocarbons, aromaticmethanol (3:1 v/v) to elute saturated hydrocarbons, aromaticmethanol (3:1 v/v) to elute saturated hydrocarbons, aromatic

  20. GAME THEORY Using randomness to confuse

    E-Print Network [OSTI]

    Zhou, Chongwu

    's NAE Moment 6 IDEA MAP: Energy Geography 8 Anatomy of a Video Game 9 Saving Da Vinci's Last Supper Department of Electrical Engineering. This is an unprecedented tragedy in our history.Words are not enough can't comprehend how the lives of two young people, full of potential, full of energy, have been taken

  1. Society of Petroleum Engineers Oil Deposits in Diatomites: A New Challenge for Subterranean Mechanics

    E-Print Network [OSTI]

    Patzek, Tadeusz W.

    Society of Petroleum Engineers SPE 75230 Oil Deposits in Diatomites: A New Challenge for Subterranean Mechanics G. I. Barenblatt1 , For. Mem. RS, NAE, NAS, T. W. Patzek2 , SPE, V. M. Prostokishin3 and D. B. Silin4 Copyright 2002, Society of Petroleum Engineers, Inc. This paper was prepared

  2. Broadening Participation in Engineering

    E-Print Network [OSTI]

    Tilbury, Dawn

    ;5 Renewable Energy Technologies Solar Photovoltaic Power & Fuels Biomass Conversion, Biofuels & Bio-Energy Wind & Wave Power Renewable Energy Emphasis Areas algae biomass wind solar #12;6 NAE Grand Challenges of scientific discovery Make solar energy economical Provide energy from fusion Develop carbon sequestration

  3. THE VITERBI USCViterbi//Engineer

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    Zhou, Chongwu

    -engineering the brain and making solar energy economical. For more on the 14 NAE Grand Challenges, visit wwwTHE VITERBI ENERgy TABlEAu USCViterbi//Engineer UsC PResidenT sTeven b. samPle On en simultaneously · Network of more than 1,000 professional students · M.S. students receive identical curriculum

  4. College of Engineering Energy Strategic Planning Committee: Interim Report

    E-Print Network [OSTI]

    Fang, Yuguang "Michael"

    combustion, solid-state lighting, clean coal technologies, and hybrid electrical vehicles. The engineering), and the recently completed NAE report, America's Energy Future: Technology and Transformation (Shapiro, 2009 fusion, 3) Develop carbon sequestration methods, and 4) Provide access to clean water. The 2008 DOE

  5. NatioNal Heart, luNg aNd Blood iNstitute National Asthma Education and Prevention Program

    E-Print Network [OSTI]

    Shen, Jun

    NatioNal Heart, luNg aNd Blood iNstitute National Asthma Education and Prevention Program NAEPP, Lung and Blood Institute Information Center (301) 251-1222 http://www.nhlbi.nih.gov NaePP school Materials http://www.nhlbi.nih.gov/health/prof/lung/ allergy & asthma Network Mothers of asthmatics (800

  6. LIFE Economics and Delivery Pathway Presentation to

    E-Print Network [OSTI]

    LIFE Economics and Delivery Pathway Presentation to National Research Council's review is necessary (but not sufficient) for economic viability Minimumforeconomics Cost and risk to buy additional systems approach is required to develop an economically viable plant design Anklam--NAS/NAE, January 29

  7. Inverting multispectral thermal time series images of volcanic eruptions for lava emplacement models

    E-Print Network [OSTI]

    Barnie, T. D.; Oppenheimer, C.

    2015-06-04

    is small – this can be 434 considered to model a situation in which there is a lot of ‘churn’ at the hot surface and most 435 material is removed in some way. In Figure 10 the NAE has a the same support at high temperatures 436 as the previous ‘complex...

  8. Westover ARB Fuel Hydrant System Upgrade set to begin

    E-Print Network [OSTI]

    US Army Corps of Engineers

    can go to recharge your batteries? Do you know who you will call if you need help getting there? · Do://www.nae.usace.army.mil/news/yankee.htm. ON THE COVER: Congr. Richard Neil speaks at the project announcement at Westover Air Reserve Base. (Photo

  9. Thinking inside the “box”: Development and implementation of a novel automated radiosynthesizer for 18F-labeled positron emission tomography tracers

    E-Print Network [OSTI]

    Lazari, Mark Saul

    2015-01-01

    cartridges ..solid-phase extraction cartridges. Nuclear Medicine andwithin a disposable SPE cartridge and subsequently eluting

  10. Enhanced drug delivery capabilities from stents coated with absorbable polymer and crystalline drug

    E-Print Network [OSTI]

    Carlyle, Wenda C.

    Current drug eluting stent (DES) technology is not optimized with regard to the pharmacokinetics of drug delivery. A novel, absorbable-coating sirolimus-eluting stent (AC-SES) was evaluated for its capacity to deliver drug ...

  11. NET PRED UTILITY

    Energy Science and Technology Software Center (OSTI)

    002602IBMPC00 Normalized Elution Time Prediction Utility  http://omics.pnl.gov/software/NETPredictionUtility.php 

  12. Concentration of perrhenate and pertechnetate solutions

    DOE Patents [OSTI]

    Knapp, Furn F. (Oak Ridge, TN); Beets, Arnold L. (Clinton, TN); Mirzadeh, Saed (Knoxville, TN); Guhlke, Stefan (Bonn, DE)

    1998-01-01

    A method of preparing a concentrated solution of a carrier-free radioisotope which includes the steps of: a. providing a generator column loaded with a composition containing a parent radioisotope; b. eluting the generator column with an eluent solution which includes a salt of a weak acid to elute a target daughter radioisotope from the generator column in a first eluate. c. eluting a cation-exchange column with the first eluate to exchange cations of the salt for hydrogen ions and to elute the target daughter radioisotope and a weak acid in a second eluate; d. eluting an anion-exchange column with the second eluate to trap and concentrate the target daughter radioisotope and to elute the weak acid solution therefrom; and e. eluting the concentrated target daughter radioisotope from the anion-exchange column with a saline solution.

  13. Concentration of perrhenate and pertechnetate solutions

    DOE Patents [OSTI]

    Knapp, F.F.; Beets, A.L.; Mirzadeh, S.; Guhlke, S.

    1998-03-17

    A method is described for preparing a concentrated solution of a carrier-free radioisotope which includes the steps of: (a) providing a generator column loaded with a composition containing a parent radioisotope; (b) eluting the generator column with an eluent solution which includes a salt of a weak acid to elute a target daughter radioisotope from the generator column in a first eluate; (c) eluting a cation-exchange column with the first eluate to exchange cations of the salt for hydrogen ions and to elute the target daughter radioisotope and a weak acid in a second eluate; (d) eluting an anion-exchange column with the second eluate to trap and concentrate the target daughter radioisotope and to elute the weak acid solution therefrom; and (e) eluting the concentrated target daughter radioisotope from the anion-exchange column with a saline solution. 1 fig.

  14. Method for enhanced accuracy in predicting peptides using liquid separations or chromatography

    DOE Patents [OSTI]

    Kangas, Lars J.; Auberry, Kenneth J.; Anderson, Gordon A.; Smith, Richard D.

    2006-11-14

    A method for predicting the elution time of a peptide in chromatographic and electrophoretic separations by first providing a data set of known elution times of known peptides, then creating a plurality of vectors, each vector having a plurality of dimensions, and each dimension representing the elution time of amino acids present in each of these known peptides from the data set. The elution time of any protein is then be predicted by first creating a vector by assigning dimensional values for the elution time of amino acids of at least one hypothetical peptide and then calculating a predicted elution time for the vector by performing a multivariate regression of the dimensional values of the hypothetical peptide using the dimensional values of the known peptides. Preferably, the multivariate regression is accomplished by the use of an artificial neural network and the elution times are first normalized using a transfer function.

  15. Plan of sugar centrals on island of Cuba 

    E-Print Network [OSTI]

    Sinclair Cuba Oil Company

    1951-01-01

    Jardin Botanique de Buitenzorg. Leide. Ann. M?d., Caen.?L'Ann?e M?dicale. Journal de la Soci?t? de M?decine de Caen et du Calvados. Caen. Ann. Med. Int. Fenn.?Annales Medicinae Inter- nae Fenniae. Helsinki. Ann, R. Staz. Bacol, Sper. Padova.... Kartoffelbeu. ?Arbeiten des Forschungsinstitutes f?r Kartoffelbau. Berlin. Arb. Med. TJniv. Okayama.?Arbeiten aus der Medizinischen Universit?t zu Okayama. [Okayama]. ?rbok Norske Vidensk.-Akad. Oslo.??rbok Det Norske Videnskaps-Akademi i Oslo. Oslo...

  16. Motiv identitete v romanih Fran?iška in Prijateljstvo pisatelja Fulvia Tomizze

    E-Print Network [OSTI]

    Starc, Maja

    2013-09-17

    Osti, in sicer da »so Tomizzevi romani navadno avtobiografski, ali kot pove v enem izmed njih, pi- sanje o lastnem življenju /…/« (Osti 2002: 116). Naše trditve lahko podkrepimo še z besedami Cirila Zlobca, dobitnika nagrade Fulvio Tomizza, ki pravi.... Josip OSTI, 2002: Slovenci v prozi Fulvia Tomizze. Sodobnost 1, 115–120. Marija PIRJEVEC, 2011: Tržaški književni razgledi. Trst: Mladika. Milan RAKOVEC, 2012: Vsi smo manjšinci. Primorske novice 15, 19. 1. 2012. Gian Antonio STELLA, 1992: Pogovor...

  17. Method of degrading trinitrotoluene

    DOE Patents [OSTI]

    Tyndall, Richard L. (Clinton, TN); Vass, Arpad (Oak Ridge, TN)

    1996-01-01

    A method of eluting trinitrotoluene (TNT) from soil using a dispersant from bacterial intra-amoebic isolate 1s, ATCC 75229.

  18. Method of degrading trinitrotoluene

    DOE Patents [OSTI]

    Tyndall, R.L.; Vass, A.

    1996-01-16

    A method is disclosed of eluting trinitrotoluene (TNT) from soil using a dispersant from bacterial intra-amoebic isolate 1s, ATCC 75229.

  19. Biochemical and Functional Characterization of the GH3 Amino Acid-Conjugase PBS3 of Arabidopsis thaliana

    E-Print Network [OSTI]

    Okrent, Rachel Allegra

    2010-01-01

    eluted at 8.3 min. The reaction velocity with each substratecatalyzed by PBS3. The reaction velocities were determinedled to substantially lower reaction velocity with ortho

  20. Application Note Immunoprecipitation with

    E-Print Network [OSTI]

    Lebendiker, Mario

    alternative suppliers. CB DA YY Y YY Y Y Y Y Dynabeads Protein A or G ® ® Dynabeads Protein Wash and elute

  1. Cells containing solvated electron lithium negative electrodes

    SciTech Connect (OSTI)

    Uribe, F.A.; Semkow, K.W.; Sammells, A.F. (Eltron Research, Incorporated, Aurora, IL (US))

    1989-12-01

    Preliminary work performed on a novel solvated electron lithium negative electrode which may have application in either high energy density secondary or reserve battery systems is discussed. The lithium electrode investigated consisted of lithium initially dissolved in liquid ammonia to give a solvated electron solution. Containment of this liquid negative active material from direct contact with a liquid nonaqueous electrolyte present in the cell positive electrode compartment was addressed via the use of a lithium intercalated electronically conducting ceramic membrane of the general composition Li{sub x}WO{sub 2}(0.1{lt}x{lt} 1.0). Secondary electrochemical cells having the general configuration Li,NH{sub 3}/Li{sub x}WO{sub 2}NAE/TiS{sub 2} using nonaqueous electrolytes (NAE) based upon both propylene carbonate and 2Me-THF. Depending upon initial lithium activity in the negative electrode compartments the cell possessed an initial open-circuit potential (OCP 3.44V). Both cells, which were operated at ambient pressure (low temperature) and ambient temperature (high pressure) showed evidence for electrochemical reversibility.

  2. Optimal drug release schedule for in-situ radiosensitization of image guided permanent prostate implants

    E-Print Network [OSTI]

    Sridhar, Srinivas

    of image guided 125 I prostate brachytherapy. Spacers used in permanent implants may be manufactured from such a constraint, is not known. This work determines the optimal elution schedules for 125 I prostate brachytherapy. The interaction between brachytherapy dose distributions and drug distribution around drug eluting spacers

  3. Separation of niobium and tantalum using a chelating ion exchange resin with N-benzoyl phenyl hydroxyl amine as functional group

    SciTech Connect (OSTI)

    Pobi, M.; Das, J. )

    1993-04-01

    Niobium is separated from Ta and V by elution with 0.5 M HF in a column of chelating resin containing N-benzoyl-N-phenyl-hydroxylamine (NBPHA) as a function group. Niobium and tantalum can also be separated using their differential distribution coefficient and elution behavior, monitored by radiometric and also be spectrophotometric methods. 15 refs., 1 fig., 2 tabs.

  4. Technetium-99m generator system

    DOE Patents [OSTI]

    Mirzadeh, S.; Knapp, F.F. Jr.; Collins, E.D.

    1998-06-30

    A {sup 99}Mo/{sup 99m}Tc generator system includes a sorbent column loaded with a composition containing {sup 99}Mo. The sorbent column has an effluent end in fluid communication with an anion-exchange column for concentrating {sup 99m}Tc eluted from the sorbent column. A method of preparing a concentrated solution of {sup 99m}Tc includes the general steps of: (a) providing a sorbent column loaded with a composition containing {sup 99}Mo, the sorbent column having an effluent end in fluid communication with an anion-exchange column; (b) eluting the sorbent column with a salt solution to elute {sup 99m}Tc from the sorbent and to trap and concentrate the eluted {sup 99m}Tc on the ion-exchange column; and (c) eluting the concentrated {sup 99m}Tc from the ion-exchange column with a solution comprising a reductive complexing agent. 1 fig.

  5. Commercial Fertilizers in 1937-38. 

    E-Print Network [OSTI]

    Ogier, T. L. (Thomas Louis); Asbury, S. E. (Samuel E.); Fraps, G. S. (George Stronach)

    1938-01-01

    ................................................ Comparing Costs of Fertilizer ...... 12 Fertilizer Analyses to be Sold in 1938-39 ............................................................ 12 Free Analysis... .......................................................................................................... 1.3 Analysis of Fertilizers, 1937-38 ............................................................................ 13 Averages Below Guarantee .................................................... ...... 15 Non-Acid Forming Fertilizers...

  6. Atom-at-a-time radiochemical separations of the heaviest elements: Lawrencium chemistry

    SciTech Connect (OSTI)

    Hoffman, D.C.; Henderson, R.A.; Gregorich, K.E.; Bennett, D.A.; Chasteler, R.M.; Gannett, C.M.; Hall, H.L.; Lee, D.M.; Nurmia, M.J.; Silva, R.J.

    1987-04-01

    The isotope /sup 260/Lr produced in reactions of /sup 18/O with /sup 249/Bk was used to perform chemical experiments on lawrencium to learn more about its chemical properties. These experiments involved extractions with thenoyl trifluoroacetate (TTA), ammonium alpha-hydroxyisobutyrate (HIB) elution from a cation exchange resin column, and reverse-phase chromatography using hydrogen di(2-ethylhexyl)orthophosphoric acid (HDEHP) to investigate the chemical properties of Lr. The results from the HIB elutions also give information about the ionic radius of Lr(III) which was found to elute very close to Er. An attempt to reduce Lr(III) was also made.

  7. Luminal flow amplifies stent-based drug deposition in arterial

    E-Print Network [OSTI]

    Levine, Evan G.

    Background: Treatment of arterial bifurcation lesions using drug-eluting stents (DES) is now common clinical practice and yet the mechanisms governing drug distribution in these complex morphologies are incompletely ...

  8. Computational model of local intravascular drug delivery

    E-Print Network [OSTI]

    Balakrishnan, Brinda

    2007-01-01

    Drug-eluting stents (DES) virtually eradicate the clinical phenomena of vessel restenosis; yet, they also increase the short and long term risks for stent thrombosis. To improve their safety and efficacy, it is critical ...

  9. Impact of flow pulsatility on arterial drug distribution in stent-based therapy

    E-Print Network [OSTI]

    O'Brien, Caroline C.

    Drug-eluting stents reside in a dynamic fluid environment where the extent to which drugs are distributed within the arterial wall is critically modulated by the blood flowing through the arterial lumen. Yet several factors ...

  10. Evaluation of iron-deficiency stress response of maize 

    E-Print Network [OSTI]

    Choi, Yim Hong

    1996-01-01

    solubilizing capacity of chelate. A high-performance liquid chromatography (HPLC) procedure with a reverse-phase gradient elution system using either sodium or ammonium acetate and 60 % acetonitrile as the eluents, with precolumn derivatization...

  11. Painting blood vessels and atherosclerotic plaques with an adhesive drug depot

    E-Print Network [OSTI]

    Kastrup, Christian

    The treatment of diseased vasculature remains challenging, in part because of the difficulty in implanting drug-eluting devices without subjecting vessels to damaging mechanical forces. Implanting materials using adhesive ...

  12. Constitutive modeling for biodegradable polymers for application in endovascular stents 

    E-Print Network [OSTI]

    da Silva Soares, Joao Filipe

    2008-10-10

    Percutaneous transluminal balloon angioplasty followed by drug-eluting stent implantation has been of great benefit in coronary applications, whereas in peripheral applications, success rates remain low. Analysis of healing ...

  13. Specific and general binding in arterial drug delivery

    E-Print Network [OSTI]

    Levin, Andrew D. (Andrew David), 1976-

    2005-01-01

    Drug-eluting stents have emerged as the most effective method for treating restenosis following percutaneous coronary interventions. This thesis investigates how drugs with similar physiochemical properties but different ...

  14. Stent Thrombogenicity Early in High Risk Interventional Settings is Driven by Stent Design and Deployment, and Protected by Polymer-Drug Coatings

    E-Print Network [OSTI]

    Kolandaivelu, Kumaran

    Background—Stent thrombosis is a lethal complication of endovascular intervention. Concern has been raised about the inherent risk associated with specific stent designs and drug-eluting coatings, yet clinical and animal ...

  15. Atmos. Chem. Phys., 10, 13151327, 2010 www.atmos-chem-phys.net/10/1315/2010/

    E-Print Network [OSTI]

    Meskhidze, Nicholas

    burning were studied. HULIS was obtained as the water-soluble, methanol-elutable material isolated from- pogenic aromatics. The rural HULIS revealed weak optical activity, which may be associated with one

  16. CX-011544: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Innovative Elution Processes for Recovering Uranium and Transition Metals from Amidoxime-based Sorbents CX(s) Applied: B3.6 Date: 12/03/2013 Location(s): Idaho Offices(s): Idaho Operations Office

  17. New tools for target identification by affinity chromatography 

    E-Print Network [OSTI]

    Landi, Felicetta

    2011-06-27

    The recovery of the selected biological material in affinity-based separations relies on reversing the biological interaction responsible for the binding. General elution methods which are independent of the bioaffinity ...

  18. Oxidative Coupling of ortho-Aminophenols and Anilines for the Application of Labeling Proteins with Fluorine-18

    E-Print Network [OSTI]

    Behrens, Christopher Richard

    2012-01-01

    a cation exchange column and trapped on a C18 cartridge.was eluted from the C18 cartridge with a methanolic solutionbe trapped on the C18 cartridge. Passage through the cation

  19. USA and Canada Tel (800) 526-7319

    E-Print Network [OSTI]

    Lebendiker, Mario

    ..................................................................5 Column preparation 5 Column chromatography 6 Strep·Tactin Cartridges Protocol...........................................................................6 Cartridge preparation 6 Cartridge chromatography 6 Strep·Tactin Cartridges FPLC Protocol .................................................................6 Cartridge chromatography 6 Processing Sample after Elution

  20. Synthesis of a proteasome inhibitor containing a [Gamma]- lactam-[Beta]-lactone fused ring system

    E-Print Network [OSTI]

    Urbina, Armando

    2009-01-01

    Biotage prepacked silica gel cartridges on Biotage Horizoncolumn chromatography (Biotage silica gel cartridge, Si75L,800 g cartridge, eluted with 0% to 50 % gradient of EtOAc/

  1. Drug deposition and distribution in healthy and atherosclerotic arteries and in models of atherosclerosis following bulk or stent-based drug delivery

    E-Print Network [OSTI]

    Vukmirovic, Neda

    2007-01-01

    Drug eluting stents have revolutionized the practice of medicine and the landscape of medical devices. Yet, more than four years after introduction clinical trial data and clinical use have still not fully clarified what ...

  2. Air quality implications associated with the selection of power plants in the Pacific Northwest

    SciTech Connect (OSTI)

    Baechler, M.C.; Glantz, C.S.; Edelmen, P.C.

    1993-11-01

    This assessment models emission inventories and pollutant emission rates for fossil fuel power plants. Ground-level air concentration of nitrogen oxides, sulfur dioxide and TSP are predicted. Pollutant deposition, non-acidic deposition, acidic deposition, ozone impacts, and visibility attenuation are considered. Human health effects, wildlife effects, effects on plants and crops, and residual environmental impacts are estimated from predicted emissions.

  3. Pilot-Scale Fermentation and Laboratory Nutrient Studies on Mixed-Acid Fermentation 

    E-Print Network [OSTI]

    Smith, Aaron Douglas

    2011-08-08

    solids (NAVS). C/N ratios were based on non-acid carbon (CNA). A blend of 93% paper and 7% wet CM (dry basis) with a C/N ratio of 37 g CNA/g N had the highest culture yield (0.21 g acidproduced/g NAVSinitial), total acid productivity (0.84 g acidproduced/(Lliq·d...

  4. Isolation, identification and quantitation of deoxycorticosterone and 21-hydroxy-5 ?-pregnane-3, 20-dione in the prepartum and postpartum mare 

    E-Print Network [OSTI]

    Dunn, Ethel Lynn

    1977-01-01

    -pregnen-3, 20-dione deoxycorticosterone DOC 21-hydroxy-4-pregnen-3, 20-dione acetate deoxycorticosterone DOCA acetate 2l-hydroxy-5o-pregnane-3, 20-diane 21-hydroxy-5o-pregnane-3, 20-dione acetate 17-hydroxy-5a-pregnane-3, 20-dione Su, 21 OH P 5u... eluted solvent 1 - 3. 5 4-10 3. 5 7. 0 discard O. SX 5a, 21 OH P 0. 5% The material that was insoluble in 0. 5% ethyl acetate in isooctane was applied to the column with 250 ul (2x) of 5l ethyl acetate in iso- octane. The following elution procedure...

  5. Method and system for radioisotope generation

    SciTech Connect (OSTI)

    Toth, James J.; Soderquist, Chuck Z.; Greenwood, Lawrence R.; Mattigod, Shas V.; Fryxell, Glen E.; O'Hara, Matthew J.

    2014-07-15

    A system and a process for producing selected isotopic daughter products from parent materials characterized by the steps of loading the parent material upon a sorbent having a functional group configured to selectively bind the parent material under designated conditions, generating the selected isotopic daughter products, and eluting said selected isotopic daughter products from the sorbent. In one embodiment, the process also includes the step of passing an eluent formed by the elution step through a second sorbent material that is configured to remove a preselected material from said eluent. In some applications a passage of the material through a third sorbent material after passage through the second sorbent material is also performed.

  6. Molecular Methods in Microbial Ecology Contact Info: Julie Huber, jhuber@mbl.edu

    E-Print Network [OSTI]

    Vallino, Joseph J.

    methods, Tour Bay Paul Center Readings: Head et al. 1998. Microbial Ecology 35: 1-21. #12;Day 1 DNA Partial genomes © 2012 Pearson Education, Inc. #12;Head et al. 1998 #12;Head et al. 1998 #12;DNA several times with alcohol 6. Elute DNA off membrane with pH 8, low-salt buffer #12;Choosing a Depth

  7. LLaannggeerrhhaannss LLaabb PPrroottooccoollss Genewiz Sample prep protocol.docx revised 9/27/13 by JW Page 1 of 5

    E-Print Network [OSTI]

    Langerhans, Brian

    and eluted in water, or by enzymatic treatment. Please use gel extraction if you have more than one product extraction (if extra bands are present) or by using ExoSAP-IT. Gel extraction: use kit, usually Omega Bio for Genewiz Sequencing" below. Genewiz says: PCR products should be purified by either gel extraction

  8. Evaluation and comparison of SuperLig{reg_sign} 644, resorcinol-formaldehyde and CS-100 ion exchange materials for the removal of cesium from simulated alkaline supernate

    SciTech Connect (OSTI)

    Brown, G.N.; Bray, L.A.; Eloviche, R.J.; Bruening, R.L.; Decker, R.M.; Kafka, T.M.; White, L.R.

    1995-03-01

    PNL evaluated three polymeric materials for Cs removal efficiency from a simulated Hanford Neutralized Current Acid Waste (NCAW) supernatant liquid using 200 mL ion exchange columns. Cs loadings (mmole Cs/g resin) were 0.20, 0.18, and 0.039 for Super Lig 644, R-F, and CS-100 (0.045, 0.070, 0.011 mmole Cs/mL resin). Elution of each resin material with 0.5 M HNO{sub 3} required 3.5, 7.0, and 3.2 cv to reach 0.1 C/C{sub 0} for the respective materials, resulting in volume compressions of 27, 20, and 6.9. Peak Cs concentrations during elution was 185, 38.5, and 27.8 C/C{sub 0}. SuperLig 644 had the highest Cs loading per gram in NCAW and the greatest volume compression on aci elution. Because of high density and poor elution, R-F had the highest Cs loading per unit volume and lower volume compression. CS-100, the baseline material for Cs removal at Hanford, was inferior to both SuperLig 644 and R-F in terms of Cs loading and selectivity over sodium.

  9. In-gel digestion of immunoprecipitated proteins separated by SDS-PAGE

    E-Print Network [OSTI]

    Trinkle-Mulcahy, Laura

    In-gel digestion of immunoprecipitated proteins separated by SDS-PAGE (Lamond Lab / April 2008) ! Perform all the pipetting steps in a laminar flow hood. We routinely do our digestions in our TC room samples from beads NOTE: To improve elution of proteins from beads and to save time during the digestion

  10. AIL Group, GRE, University of Dundee 2014 In-gel digestion of proteins separated by

    E-Print Network [OSTI]

    Lamond, Angus I.

    AIL Group, GRE, University of Dundee 2014 In-gel digestion of proteins separated by SDS a SILAC IP (NOTE: To improve elution of proteins from beads and to save time during the digestion, we of cysteine-containing peptides from in-gel digests by opening the structure of proteins (disrupting

  11. Speciation and transport of anthropogenic 129Iodine and natural 127Iodine in surface and subsurface environments 

    E-Print Network [OSTI]

    Schwehr, Kathleen Ann

    2005-02-17

    ................................... 29 2.2 Mobile phase gradient elution profile ........................................................ 30 2.3 Iodine speciation in the vertical profile of a warm core ring, Gulf of Mexico, 26o 0.04? N, 95o 20? W. Collection was July 9...

  12. Physics Contribution Nanoparticle-Based Brachytherapy Spacers for

    E-Print Network [OSTI]

    Sridhar, Srinivas

    Physics Contribution Nanoparticle-Based Brachytherapy Spacers for Delivery of Localized Combined of brachytherapy spacers for in situ release of drug eluting nanoparticles to provide tissue residence therapy to enhance the therapeutic ratio Purpose: In radiation therapy (RT), brachytherapy-inert source

  13. Journal of Clinical Investigation Vol. 42, No. 12, 1963

    E-Print Network [OSTI]

    Alper, Chester A.

    Journal of Clinical Investigation Vol. 42, No. 12, 1963 THE METABOLISM OF GAMMA GLOBULINS- ent study was undertaken to delineate and com- pare simultaneously the metabolism of normal gamma on diethylaminoethyl cellulose (DEAE) with gradient elution (11) from 0.005 M Tris-phosphate, pH 8.0, to 0.3 M Tris

  14. GLUTAMATE DEHYDROGENASE IN THE DIGESTIVE TRACT OF SHEEP

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    was divided into two frac- tions. The first fraction (peak I) flowed from the column at a low NaCl gradient. The second fraction (peak II) was eluted by a simi- lar NaCl gradient as the enzymic fraction puri- fied Metabolism in Rumen Bacteria and Mucosa froc Sheep Fed Soya Protein or Urea. J. Nutr., 100, 161-169. FRIEDEN

  15. AdenoPACKTM Adenovirus (Ad5) purification and concentration kit for

    E-Print Network [OSTI]

    Lebendiker, Mario

    only. Warning: The virus purified using this kit is capable of infecting human or animal cells particles can be further purified by washing away non- specifically bound proteins, before elution within 1 pH 7.4 (PBS) Ethanol / dry ice bath or -80°C freezer Water bath at 25°C Retort stand and clamp

  16. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

    E-Print Network [OSTI]

    Hibbett, David S.

    polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Overview remove trace contaminants such as residual polysaccharides and pure DNA is eluted in water or low ionic

  17. Preliminary Ion Exchange Modeling for Removal of Cesium from Hanford Waste Using SuperLig 644 Resin

    SciTech Connect (OSTI)

    Hamm, L.L.

    2000-08-23

    A proposed facility is being designed for the immobilization of Hanford high-level radioactive waste. One unit process in the facility is designed to remove radioactive cesium by ion-exchange from the strongly alkaline aqueous phase. A resin specifically designed with high selectivity of cesium under alkaline conditions is being investigated. The resin also is elutable under more acidic conditions. The proposed design of the facility consists of two sets of two packed columns placed in series (i.e., a lead column followed by a lag (guard) column configuration). During operation, upon reaching a specified cesium concentration criterion at the exit of the lag column, operation is switched to the second set of lead and lag columns. The cesium-loaded lead column is processed (i.e., washed and eluted) and switched to the lag position. the previous lag column is then placed in the lead position (without eluting) and the system is ready for use in the next cycle. For a well designed process, the loading and elution processes result in significant volume reductions in aqueous high-level waste.

  18. Chromatofocusing Douglas D Frey,University of Maryland Baltimore County, Maryland, USA

    E-Print Network [OSTI]

    Frey, Douglas D.

    Chromatofocusing Douglas D Frey,University of Maryland Baltimore County, Maryland, USA Chittoor R Narahari,University of Maryland Baltimore County, Maryland, USA Ronald C Bates, University of Maryland Baltimore County, Maryland, USA Chromatofocusing is a form of gradient elution chromatography performed

  19. MedicalPhysicsWeb PHYSICS IN MEDICINE & BIOLOGY

    E-Print Network [OSTI]

    Sridhar, Srinivas

    MedicalPhysicsWeb PHYSICS IN MEDICINE & BIOLOGY Oct 11, 2010 D K Nagesha et al 2010 Phys. Med. Biol efficiency of IGRT can be further enhanced by biological in-situ dose painting (BIS-IGRT) of radiosensitizers for biological in-situ image-guided radio therapy (BIS-IGRT) Page 1 of 2Radiosensitizer-eluting nanocoatings

  20. Calcite dissolution and Ca/Na ion-exchange reactions in columns with different flow rates through high ESR soil 

    E-Print Network [OSTI]

    Navarre, Audrey

    1999-01-01

    min?¹ under conditions of saturated flow. Column eluate was monitored for pH, carbonate alkalinity, and Na, Ca and Cl concentrations to evaluate the elution of SAR 10 solution, dissolution of CaCO? and exchange of Na by Ca on the cation...

  1. 10 to 70% methanol in 50 mM KH2PO4 over 25 min, 10 ml/min, monitor at 380 nm). Next, the HPLC-

    E-Print Network [OSTI]

    Gao, Jinming

    10 to 70% methanol in 50 mM KH2PO4 over 25 min, 10 ml/min, monitor at 380 nm). Next, the HPLC- purified mixture was desalted on the same column (methanol was removed on a rotary evaporator, and the sample loaded in H2O and eluted with 90% methanol) and lyophilized, yielding the purified Nvoc

  2. The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivophosphorylation site

    E-Print Network [OSTI]

    de Graaf, Katrin; Czajkowska, Hanna; Rottmann, Sabine; Packman, Len C; Lilischkis, Richard; Luscher, Bernhard; Becker, Walter

    2006-03-02

    , Germany). Proteins were eluted under native conditions (reduced glutathione or imidazol). His6-tagged proteins were purified by gel fil- tration through a Sephadex G25 column (NAP™-5 col- umn, Amersham Bioscience) and equilibrated in 10 mM Tris pH 7.4, 100...

  3. Delivery Vehicles for Zerovalent Metal Nanoparticles in Soil and Groundwater

    E-Print Network [OSTI]

    Delivery Vehicles for Zerovalent Metal Nanoparticles in Soil and Groundwater Bettina Schrick hydrocarbons in groundwater and soils. The transport of Fe/C nanoparticles was studied by elution through and the groundwater level, in some cases reaching the underlying saturated zone. As hydrophobic sparingly soluble

  4. Osmium-191/iridium-191m radionuclide

    DOE Patents [OSTI]

    Knapp, F.F. Jr.; Butler, T.A.; Brihaye, C.

    1985-08-26

    A generator system to provide iridium-191m for clinical imaging applications comprises an activated carbon adsorbent loaded with a compound containing the parent nuclide, osmium-191. The generator, which has a shelf-life in excess of two weeks and does not require a scavenger column, can be eluted with physiologically compatible saline. 4 figs. 3 tabs.

  5. Journal of Chromatography, 553 (1991) 93-99 Elsevier Science Publishers B.V., Amsterdam

    E-Print Network [OSTI]

    Miksik, Ivan

    1991-01-01

    on a cellulose column, first eluted with n-butanol-acetic acid-water to wash out other amino acids samples. The tissue is treated with cold 10% trichloroacetic acid to remove collagen and hydrolysed in HCI vapours in sealed vials. Preseparation of desmosines from tissue acid hydrolysates is performed

  6. Liquid chromatographic extraction medium

    DOE Patents [OSTI]

    Horwitz, E.P.; Dietz, M.L.

    1994-09-13

    A method and apparatus are disclosed for extracting strontium and technetium values from biological, industrial and environmental sample solutions using a chromatographic column. An extractant medium for the column is prepared by generating a solution of a diluent containing a Crown ether and dispersing the solution on a resin substrate material. The sample solution is highly acidic and is introduced directed to the chromatographic column and strontium or technetium is eluted using deionized water. 1 fig.

  7. Method for liquid chromatographic extraction of strontium from acid solutions

    DOE Patents [OSTI]

    Horwitz, E. Philip (Naperville, IL); Dietz, Mark L. (Evanston, IL)

    1992-01-01

    A method and apparatus for extracting strontium and technetium values from biological, industrial and environmental sample solutions using a chromatographic column is described. An extractant medium for the column is prepared by generating a solution of a diluent containing a Crown ether and dispersing the solution on a resin substrate material. The sample solution is highly acidic and is introduced directed to the chromatographic column and strontium or technetium is eluted using deionized water.

  8. Liquid chromatographic extraction medium

    DOE Patents [OSTI]

    Horwitz, E. Philip (Naperville, IL); Dietz, Mark L. (Evanston, IL)

    1994-01-01

    A method and apparatus for extracting strontium and technetium values from biological, industrial and environmental sample solutions using a chromatographic column is described. An extractant medium for the column is prepared by generating a solution of a diluent containing a Crown ether and dispersing the solution on a resin substrate material. The sample solution is highly acidic and is introduced directed to the chromatographic column and strontium or technetium is eluted using deionized water.

  9. www.activemotif.comwww.activemotif.com Protein Purification

    E-Print Network [OSTI]

    Lebendiker, Mario

    Storage Resuspension Buffer 45 ml 175 ml Room Temp. Buffer A 33 ml 125 ml Room Temp. Elution Buffer 11 ml. Resuspension Buffer 50 mM Tris, pH 8.0, 300 mM NaCl, and 10 mM Imidazole, 0.2% Triton X-100 2. Lysozyme 100 mg concentration of 0.2% to the Resuspension Buffer. This will help the ly

  10. Method for aqueous gold thiosulfate extraction using copper-cyanide pretreated carbon adsorption

    DOE Patents [OSTI]

    Young, Courtney; Melashvili, Mariam; Gow, Nicholas V

    2013-08-06

    A gold thiosulfate leaching process uses carbon to remove gold from the leach liquor. The activated carbon is pretreated with copper cyanide. A copper (on the carbon) to gold (in solution) ratio of at least 1.5 optimizes gold recovery from solution. To recover the gold from the carbon, conventional elution technology works but is dependent on the copper to gold ratio on the carbon.

  11. Generator for ionic gallium-68 based on column chromatography

    DOE Patents [OSTI]

    Neirinckx, Rudi D. (Medfield, MA); Davis, Michael A. (Westwood, MA)

    1981-01-01

    A physiologically acceptable solution of gallium-68 fluorides, having an activity of 0.1 to 50 millicuries per milliliter of solution is provided. The solution is obtained from a generator comprising germanium-68 hexafluoride bound to a column of an anion exchange resin which forms gallium-68 in situ by eluting the column with an acid solution to form a solution containing .sup.68 Ga-fluorides. The solution then is neutralized prior to administration.

  12. Method for preparing high specific activity 177Lu

    DOE Patents [OSTI]

    Mirzadeh, Saed; Du, Miting; Beets, Arnold L.; Knapp, Jr., Furn F.

    2004-04-06

    A method of separating lutetium from a solution containing Lu and Yb, particularly reactor-produced .sup.177 Lu and .sup.177 Yb, includes the steps of: providing a chromatographic separation apparatus containing LN resin; loading the apparatus with a solution containing Lu and Yb; and eluting the apparatus to chromatographically separate the Lu and the Yb in order to produce high-specific-activity .sup.177 Yb.

  13. Method for the concentration and separation of actinides from biological and environmental samples

    DOE Patents [OSTI]

    Horwitz, E.P.; Dietz, M.L.

    1989-05-30

    A method and apparatus for the quantitative recover of actinide values from biological and environmental sample by passing appropriately prepared samples in a mineral acid solution through a separation column of a dialkyl(phenyl)-N,N-dialylcarbamoylmethylphosphine oxide dissolved in tri-n-butyl phosphate on an inert substrate which selectively extracts the actinide values. The actinide values can be eluted either as a group or individually and their presence quantitatively detected by alpha counting. 3 figs.

  14. Control of Bud Activation by an Auxin Transport Switch Supporting Information

    E-Print Network [OSTI]

    Prusinkiewicz, Przemyslaw

    % acetic acid was added to each sample. The pH was adjusted to around 2.7 with 5 µl 1 M HCl before with 1 ml of methanol and 1 ml of 1% acetic acid. The sample vial was rinsed with 100 µl 1% acetic acid% acetic acid and then eluted two times with 0.5 ml of methanol. The combined sample was evaporated

  15. Characterization and Application of Superlig 620 Solid Phase Extraction Resin for Automated Process Monitoring of 90Sr

    SciTech Connect (OSTI)

    Devol, Timothy A.; Clements, John P.; Farawila, Anne F.; O'Hara, Matthew J.; Egorov, Oleg; Grate, Jay W.

    2009-11-30

    Characterization of SuperLig® 620 solid phase extraction resin was performed in order to develop an automated on-line process monitor for 90Sr. The main focus was on strontium separation from barium, with the goal of developing an automated separation process for 90Sr in high-level wastes. High-level waste contains significant 137Cs activity, of which 137mBa is of great concern as an interference to the quantification of strontium. In addition barium, yttrium and plutonium were studied as potential interferences to strontium uptake and detection. A number of complexants were studied in a series of batch Kd experiments, as SuperLig® 620 was not previously known to elute strontium in typical mineral acids. The optimal separation was found using a 2M nitric acid load solution with a strontium elution step of ~0.49M ammonium citrate and a barium elution step of ~1.8M ammonium citrate. 90Sr quantification of Hanford high-level tank waste was performed on a sequential injection analysis microfluidics system coupled to a flow-cell detector. The results of the on-line procedure are compared to standard radiochemical techniques in this paper.

  16. Modeling Ion-Exchange Processing With Spherical Resins For Cesium Removal

    SciTech Connect (OSTI)

    Hang, T.; Nash, C. A.; Aleman, S. E.

    2012-09-19

    The spherical Resorcinol-Formaldehyde and hypothetical spherical SuperLig(r) 644 ion-exchange resins are evaluated for cesium removal from radioactive waste solutions. Modeling results show that spherical SuperLig(r) 644 reduces column cycling by 50% for high-potassium solutions. Spherical Resorcinol Formaldehyde performs equally well for the lowest-potassium wastes. Less cycling reduces nitric acid usage during resin elution and sodium addition during resin regeneration, therefore, significantly decreasing life-cycle operational costs. A model assessment of the mechanism behind ''cesium bleed'' is also conducted. When a resin bed is eluted, a relatively small amount of cesium remains within resin particles. Cesium can bleed into otherwise decontaminated product in the next loading cycle. The bleed mechanism is shown to be fully isotherm-controlled vs. mass transfer controlled. Knowledge of residual post-elution cesium level and resin isotherm can be utilized to predict rate of cesium bleed in a mostly non-loaded column. Overall, this work demonstrates the versatility of the ion-exchange modeling to study the effects of resin characteristics on processing cycles, rates, and cold chemical consumption. This evaluation justifies further development of a spherical form of the SL644 resin.

  17. Characterization of Pt-based Transition Metal Alloy Electrodes for PEFC

    SciTech Connect (OSTI)

    Uebayashi, M.; Sato, Y.; Jeyadevan, B.; Tohji, K. [Graduate School of Environmental Studies, Tohoku Univ., Aramaki aza Aoba, Aoba-ku, Sendai (Japan); Sawada, Y. [Sumitomo Corporation, 1-8-11, Harumi, Chuo-ku, Tokyo 104-8610 (Japan); Itoh, T. [Center for Interdisciplinary Research, Tohoku Univ., Aramaki aza Aoba, Aoba-ku, Sendai (Japan)

    2007-03-20

    Pt metal is generally used as electrocatalyst in polymer electrolyte fuel cells (PEFC). However, the catalytic action of Pt anode is obstructed by the adsorption of CO gas molecules on to their surface and consequently the efficiency of the fuel cell decreases. Moreover, the activity of PEFC deteriorates even if the presence of CO is as low as 100 ppm. In this paper, attempts are being made to replace the Pt with Pt-based transition metal alloys such as PtFe and PtCo to overcome the above problem and also to improve the activity of fuel cells. The PtFe/C and PtCo/C necessary for the preparation of the electrodes were synthesized by depositing PtFe and PtCo particles synthesized using the polyol process on to the surface ketjenblack. From the results of cyclic voltammetry, we could confirm that PtFe/C could be used as an alternate material of Pt/C. However, Fe ions get eluted in the electrolyte. Thus, the study that can control the elution of Fe ion is necessary. Also in case of PtCo/C, we could confirm that Co ion doesn't get to elute, however the hydrogen oxidation reaction current is quite low.

  18. Covalent modification of hepatic microsomal lipids of rats by carbon tetrachloride

    SciTech Connect (OSTI)

    Kaphalia, B.S.; Ansari, G.A. (Univ. of Texas Medical Branch, Galveston (USA))

    1989-07-01

    The present study was undertaken to isolate and identify various lipids bound to 14C label during hepatic microsomal metabolism of 14CCl4 in vitro under anaerobic conditions and in vivo in rats. The two major radioactive fractions identified by thin-layer chromatography each for neutral lipids and phospholipids from in vitro and in vivo experiments corresponded to fatty acids and triglycerides and to phosphatidylcholine (PC) and phosphatidylethanolamine (PE), respectively. Approximately 89% of the radioactivity associated with phospholipids was found in PC and PE fractions. Hydrolysis of PC and PE with phospholipase A2 released about 50% of the total radioactivity as lipid moieties corresponding to fatty acids. The radioactive neutral lipids and the lipid moieties hydrolyzed from PC and PE were methylated with boron trifluoride in methanol. These methylated lipids were separated by reversed-phase high-performance liquid chromatography (HPLC), and the elution profiles of 14C label found for the lipids obtained from in vitro experiments were similar to those from in vivo. The major radioactive fractions eluted immediately after methyl oleate were identified as trichloromethyloctadecenoic and trichloromethyleicosatrienoic acid methyl esters by chemical ionization mass spectrometry. The mass spectral analysis of these fractions also indicated the formation of dichlorocarbene adduct of oleic acid. However, similar mass spectrometric detection of trichloromethylated lipids was not evident in neutral lipids and phospholipids isolated from in vivo studies. The 14C-labeled lipids eluted as a nonpolar fraction exhibited a high molecular weight containing more than three chlorines. Dimerization and cross-linking of trichloromethylated lipids based on HPLC and mass spectral analysis are also discussed in this paper.

  19. A new osmium-191/iridium-191m generator system

    SciTech Connect (OSTI)

    Brihaye, C.; Knapp, F.F. Jr.; Butler, T.A.; Guillaume, M.

    1985-05-01

    Ir-191m (t/sub 1/2/ 4.96 sec) has very attractive properties for radionuclide angiography and is obtained from the long-lived (15 d) Os-191 parent. Because of the long parent half-life, generators could have an extended shelf-life (2-3 weeks) if the problems of low Ir-191m yields and rapidly increasing Os-191 breakthrough could be overcome. A new /sup 191/Os//sup 191m/Ir generator system has now been developed involving adsorption of K/sub 2/OsCl/sub 6/ (Os-IV) on heat-treated (800/sup 0/C) activated coconut carbon. This system gives consistently good Ir-191m yields of --35-40% for a 2 ml bolus and low Os-191 breakthrough of --1 x 10/sup -4/%/bolus over a 2-3 week period. In addition, this simple system does not require a scavenger column and no radiolysis has been detected after loading up to 1 Ci of Os-191. The generator is prepared from 140-230 mesh carbon and eluted with pH 2 - 0.9% NaCl containing 0.25% KI. The use of KI in the eluting solution is critical for generator performance. Yield and breakthrough do not change for up to 250 elutions of the prototype system. The generator eluent is neutralized to physiological pH and isotonicity with 0.13 M Tris buffer immediately prior to intravenous injection. No adverse effects have been observed in studies in normal adult volunteers. This new system has a long shelf-life and represents a simple, readily available source of Ir-191m for radionuclide angiography.

  20. A new osmium-191/iridium-191m generator system

    SciTech Connect (OSTI)

    Brihaye, C.; Knapp, F.F. Jr.; Butler, T.A.; Guillaume, M.

    1985-05-01

    Ir-191m (t1/2 4.96 sec) has very attractive properties for radionuclide angiography and is obtained from the long-lived (15 d) 0s-191 parent. Because of the long parent half-life, generators could have an extended shelf-life (2-3 weeks) if the problems of low Ir-191m yields and rapidly increasing Os-191 breakthrough could be overcome. A new /sup 191/Os//sup 191m/Ir generator system has now been developed involving adsorption of K/sub 2/OsCl/sub 6/ (0s-IV) on heat-treated (800/sup 0/C) activated coconut carbon. This system gives consistently good Ir-191m yields of approx. =35-40% for a 5 ml bolus and low Os-191 breakthrough of approx. =1x10/sup -4/% bolus over a 2-3 week period. In addition, this simple system does not require a scavenger column and no radiolysis has been detected after loading up to 1 Ci of Os-191. The generator is prepared from 140-230 mesh carbon and eluted with pH 2 - 0.9% NaCl containing 0.025% KI. The use of KI in the eluting solution is critical for generator performance. Yield and breakthrough do not change for up to 250 elutions of the prototype system. The generator eluent is neutralized to physiological pH and isotonicity with 0.13 M Tris buffer immediately prior to intravenous injection. No adverse effects have been observed in studies in normal adult volunteers. This system has a long shelf-life and represents a simple, readily available source of Ir-191m for radionuclide angiography.

  1. Rapid determination of 226Ra in emergency urine samples

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Maxwell, Sherrod L.; Culligan, Brian K.; Hutchison, Jay B.; Utsey, Robin C.; McAlister, Daniel R.

    2014-02-27

    A new method has been developed at the Savannah River National Laboratory (SRNL) that can be used for the rapid determination of 226Ra in emergency urine samples following a radiological incident. If a radiological dispersive device event or a nuclear accident occurs, there will be an urgent need for rapid analyses of radionuclides in urine samples to ensure the safety of the public. Large numbers of urine samples will have to be analyzed very quickly. This new SRNL method was applied to 100 mL urine aliquots, however this method can be applied to smaller or larger sample aliquots as needed.more »The method was optimized for rapid turnaround times; urine samples may be prepared for counting in 226Ra from the urine sample matrix, followed by removal of calcium by cation exchange separation. A stacked elution method using DGA Resin was used to purify the 226Ra during the cation exchange elution step. This approach combines the cation resin elution step with the simultaneous purification of 226Ra with DGA Resin, saving time. 133Ba was used instead of 225Ra as tracer to allow immediate counting; however, 225Ra can still be used as an option. The rapid purification of 226Ra to remove interferences using DGA Resin was compared with a slightly longer Ln Resin approach. A final barium sulfate micro-precipitation step was used with isopropanol present to reduce solubility; producing alpha spectrometry sources with peaks typically 90 %), and removes interferences effectively. The sample preparation method can also be adapted to ICP-MS measurement of 226Ra, with rapid removal of isobaric interferences.« less

  2. Washing of Sul Ross Statue 

    E-Print Network [OSTI]

    Unknown

    2011-08-17

    be detrimental to purification efficiency and/or product activity if interact with proteins (15-17). Phytic acid is present at a higher amount than other impurities, i.e. 1 mg/g in brown rice (15) and is also found within the hulls of nuts, seeds, and grains...- bed-column filled with an ion exchange resin, and the unbound substances are washed out from the column using the starting buffer. Then, the bound proteins are sequentially eluted from the column and separated from each other (28). The presence...

  3. Isolation and identification of fatty acid amides from Shengli coal

    SciTech Connect (OSTI)

    Ming-Jie Ding; Zhi-Min Zong; Ying Zong; Xiao-Dong Ou-Yang; Yao-Guo Huang; Lei Zhou; Feng Wang; Jiang-Pei Cao; Xian-Yong Wei

    2008-07-15

    Shengli coal, a Chinese brown coal, was extracted with carbon disulfide and the extract was gradiently eluted with n-hexane and ethyl acetate (EA)/n-hexane mixed solvents with different concentrations of EA in a silica gel-filled column. A series of fatty acid amides, including fourteen alkanamides (C{sub 15}-C{sub 28}) and three alkenamides (C{sub 18} and C{sub 22}), were isolated from the coal by this method and analyzed with a gas chromatography/mass spectrometry. 26 refs., 2 figs., 2 tabs.

  4. Involvement of peptides in nitrogen fixation 

    E-Print Network [OSTI]

    Ahlgren, Joy Annette

    1983-01-01

    molecular weight. 4 Effect of fixed nitrogen (Ca(NO&)z) on the nodula- tion (the number of nodules per plant), growth of nodules (mg per nodule), nitrogenase activity (acetylene reduction assay), and soyrhizin A con- tent in arginine equivalents. 22... solution and 0. 2 g of picric acid were mixed, the volume reduced to 10 approximately 20 ml by vacuum evaporation, and then centrifuged at 25, 000g for 20 min. Each of these samples was eluted from a column of 10 0 go e AG 248 e 1 . F o Rhitohiom 2~4 tc...

  5. An Engineering Evaluation of Spherical Resorcinol Formaldehyde Resin

    SciTech Connect (OSTI)

    Birdwell Jr, Joseph F; Lee, Denise L; Taylor, Paul Allen; Collins, Robert T; Hunt, Rodney Dale

    2010-09-01

    A small column ion exchange (SCIX) system has been proposed for removal of cesium from caustic, supernatant, and dissolved salt solutions stored or generated from high-level tank wastes at the US Department of Energy (DOE) Hanford Site and Savannah River Sites. In both instances, deployment of SCIX systems, either in-tank or near-tank, is a means of expediting waste pretreatment and dispositioning with minimal or no new infrastructure requirements. Conceptually, the treatment approach can utilize a range of ion exchange media. Previously, both crystalline silicotitanate (CST), an inorganic, nonelutable sorbent, and resorcinol-formaldehyde (RF), an organic, elutable resin, have been considered for cesium removal from tank waste. More recently, Pacific Northwest National Laboratory (PNNL) evaluated use of SuperLig{reg_sign} 644, an elutable ion exchange medium, for the subject application. Results of testing indicate hydraulic limitations of the SuperLig{reg_sign} resin, specifically a high pressure drop through packed ion exchange columns. This limitation is likely the result of swelling and shrinkage of the irregularly shaped (granular) resin during repeated conversions between sodium and hydrogen forms as the resin is first loaded then eluted. It is anticipated that a similar flow limitation would exist in columns packed with conventional, granular RF resin. However, use of spherical RF resin is a likely means of mitigating processing limitations due to excessive pressure drop. Although size changes occur as the spherical resin is cycled through loading and elution operations, the geometry of the resin is expected to effectively mitigate the close packing that leads to high pressure drops across ion exchange columns. Multiple evaluations have been performed to determine the feasibility of using spherical RF resin and to obtain data necessary for design of an SCIX process. The work performed consisted of examination of radiation effects on resin performance, quantification of cesium adsorption performance as a function of operating temperature and pH, and evaluation of sodium uptake (titration) as function of pH and counteranion concentration. The results of these efforts are presented in this report. Hydraulic performance of the resin and the use of eluant alternatives to nitric acid have also been evaluated and have been reported elsewhere (Taylor 2009, Taylor and Johnson 2009).

  6. Process for removing metals from water

    DOE Patents [OSTI]

    Napier, J.M.; Hancher, C.M.; Hackett, G.D.

    1987-06-29

    A process for removing metals from water including the steps of prefiltering solids from the water, adjusting the pH to between about 2 and 3, reducing the amount of dissolved oxygen in the water, increasing the pH to between about 6 and 8, adding water-soluble sulfide to precipitate insoluble sulfide- and hydroxide-forming metals, adding a containing floc, and postfiltering the resultant solution. The postfiltered solution may optionally be eluted through an ion exchange resin to remove residual metal ions. 2 tabs.

  7. Generator for gallium-68 and compositions obtained therefrom

    DOE Patents [OSTI]

    Neirinckx, Rudi D. (Medfield, MA); Davis, Michael A. (Westwood, MA)

    1981-01-01

    A generator for obtaining radioactive gallium-68 from germanium-68 bound in a resin containing unsubstituted phenolic hydroxyl groups. The germanium-68 is loaded into the resin from an aqueous solution of the germanium-68. A physiologically acceptable solution of gallium-68 having an activity of 0.1 to 50 millicuries per milliliter of gallium-68 solution is obtained. The solution is obtained from the bound germanium-68 which forms gallium-68 in situ by eluting the column with a hydrochloric acid solution to form an acidic solution of gallium-68. The acidic solution of gallium-68 can be neutralized.

  8. Synthesis of ethyl N-carbobenzoxy-L-valyl-L-valyl-4-amino-3-hydroxyoctanoate 

    E-Print Network [OSTI]

    Chesky, Elizabeth Gourley

    1982-01-01

    with 5X citric acid to pH 5. The aqueous layer was then extracted several times with ethyl acetate. The com- bined organic layer was dried over anhydrous magnesium sulfate and the solvent was removed by evaporation in vacuo. The product was a clear.../ sodium bicarbonate, dried with 29 anhydrous magnesium sulfate, and concentrated by evaporation in vacuo. The product was a pale yellow oil weighing 0. 6 gm (81K yield). The material was passed through a short column of silica gel, eluting with 2...

  9. Statistical Methods for the Analysis of Mass Spectrometry-based Proteomics Data 

    E-Print Network [OSTI]

    Wang, Xuan

    2012-07-16

    was sponsored by a subcontract from PNNL and by the NIH R25-CA-90301 training grant at TAMU. Additional support was provided by KAUST-IAMCS Innovation grant, by NIH grant DK070146 and by the National Institute of Allergy and Infectious Diseases (NIH...CMass spectrometry M/Z Mass over charge ratio NET Normalized elution time NMC Number of missed cleavage sites NTE Number of tryptic ends PEP Posterior error probability PM Potential matches PMF Probability mass function PNNL Paci c northwest national...

  10. Supporting Information Moissiard et al. 10.1073/pnas.1406611111

    E-Print Network [OSTI]

    Jacobsen, Steve

    Supporting Information Moissiard et al. 10.1073/pnas.1406611111 FLAG rbcL B atmorc6-1 - AtM ORC6 -FLAG cm t3 - cmt3/ atmorc1-3 GFP C FLAG Input Flowthrough Elution AtM ORC1 -FLAG wild-type 200 400 600MORC5 atm orc2-1 atm orc1-2 FLAG-AtM ORC2 atm orc1-2/atm orc2-1 atm orc1-2/atm orc2-1 w ild-type atm orc

  11. Laboratory studies of radionuclide migration in tuff

    SciTech Connect (OSTI)

    Rundberg, R.S.; Mitchell, A.J.; Ott, M.A.; Thompson, J.L.; Triay, I.R.

    1989-10-01

    The movement of selected radionuclides has been observed in crushed tuff, intact tuff, and fractured tuff columns. Retardation factors and dispersivities were determined from the elution profiles. Retardation factors have been compared with those predicted on the basis of batch sorption studies. This comparison forms a basis for either validating distribution coefficients or providing evidence of speciation, including colloid formation. Dispersivities measured as a function of velocity provide a means of determining the effect of sorption kinetics or mass transfer on radionuclide migration. Dispersion is also being studied in the context of scaling symmetry to develop a basis for extrapolating from the laboratory scale to the field. 21 refs., 6 figs., 2 tabs.

  12. Synovial pharmacokinetics of tulathromycin, gamithromycin and florfenicol after a single subcutaneous dose in cattle 

    E-Print Network [OSTI]

    Jones, Meredyth L; Washburn, Kevin E; Fajt, Virginia R; Rice, Somchai; Coetzee, Johann F

    2015-02-07

    as internal standards and vigorously mixed by vortex. The samples were centrifuged at 2000 rpm for 20 minutes to pellet solids. The entire diluted supernatant was applied to a solid phase extraction (SPE) cartridge, Strata X-C 33 Polymeric Strong Cation (100... with ultrapure water (1 mL), followed by 5% methanol in water (v/v) (1 mL). The SPE cartridges were dried under flow of nitrogen for 5 minutes. Florfenicol (and thiamphe- nicol) was eluted with 2 fractions of 1 mL portions of 70:30 acetonitrile: methanol into a...

  13. Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

    DOE Patents [OSTI]

    Knapp, F.F. Jr.; Lisic, E.C.; Mirzadeh, S.; Callahan, A.P.

    1994-01-04

    A generator system has been invented for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form. 1 figure.

  14. Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

    DOE Patents [OSTI]

    Knapp, F.F. Jr.; Lisic, E.C.; Mirzadeh, S.; Callahan, A.P.

    1993-02-16

    A generator system for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form.

  15. Methods, microfluidic devices, and systems for detection of an active enzymatic agent

    DOE Patents [OSTI]

    Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

    2014-10-28

    Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.

  16. Genetic Mechanisms Behind Flower Color Variation in Caulanthus Amplexicaulis var. Amplexicaulis (CAA) and Caulanthus Amplexicaulis var. Barbarae (CAB) 

    E-Print Network [OSTI]

    Lane, Rebecca Kathryn

    2013-02-06

    . Verification The candidate sequences were searched for single nucleotide polymorphisms (SNPs) between CAA and CAB that resulted in different restriction sites and primers were created to isolate these polymorphisms. Two forward primers and four reverse were... ordered: FC1-F, FC1-R3, FC1-R4, FC2-F, FC2-R2, and FC2-R3. The primers were then prepared in test tubes. First 10mM Tris was 7 added to make a concentration of 100pmol/?L, and then each was further diluted in 10mM elution buffer (EB) to a final...

  17. Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

    DOE Patents [OSTI]

    Knapp, Jr., Furn F. (Oak Ridge, TN); Lisic, Edward C. (Cookeville, TN); Mirzadeh, Saed (Knoxville, TN); Callahan, Alvin P. (Harriman, TN)

    1993-01-01

    A generator system for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form.

  18. Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

    DOE Patents [OSTI]

    Knapp, Jr., Furn F. (Oak Ridge, TN); Lisic, Edward C. (Cookeville, TN); Mirzadeh, Saed (Knoxville, TN); Callahan, Alvin P. (Harriman, TN)

    1994-01-01

    A generator system for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form.

  19. Characterization and purification of juvenile hormone acid methyltransferase 

    E-Print Network [OSTI]

    Peck, Kenneth Elden

    1989-01-01

    Column . 30 8. Effect of S-adenosylhomocysteine on JHANT Activity 9. Elution Profile from a Sephadex G-100 Column 10. Chromatography of JHAMT on a DEAE-NPR HPLC Ion Exchange 31 36 Column 39 11. Chromatography of JHAMT on a DE-52 DEAE Cellulose Ion...: 1. DEAE-NPR HPLC column. Small aliquots of active fractions from the G-100 column were injected onto a 4. 4x20 mm TSK gel DEAE NPR ion-exchange column (Hewlett Packard, Avondale, Penn) with a Beckman HPLC system (Berkeley, CA) at 0. 5 ml...

  20. Ion exchange purification of scandium

    DOE Patents [OSTI]

    Herchenroeder, Laurie A. (Noblesville, IN); Burkholder, Harvey R. (Ames, IA)

    1990-10-23

    An improvement in purification of scandium through ion exchange chromatography is disclosed in which the oxidation potential of the eluting solution is altered by the addition of potassium chlorate or ammonium chloride so that removal of contaminants is encouraged. The temperature, pH and concentration of the eluent HEDTA are controlled in order to maintain the scandium in the column while minimizing dilution of the scandium band. Recovery of scandium is improved by pumping dilute scandium over the column prior to stripping the scandium and precipitation. This eliminates the HEDTA ion and other monovalent cations contaminating the scandium band. This method maximizes recovery of scandium while maintaining purity.

  1. Sub-to super-ambient temperature programmable microfabricated gas chromatography column

    DOE Patents [OSTI]

    Robinson, Alex L.; Anderson, Lawrence F.

    2004-03-16

    A sub- to super-ambient temperature programmable microfabricated gas chromatography column enables more efficient chemical separation of chemical analytes in a gas mixture by combining a thermoelectric cooler and temperature sensing on the microfabricated column. Sub-ambient temperature programming enables the efficient separation of volatile organic compounds and super-ambient temperature programming enables the elution of less volatile analytes within a reasonable time. The small heat capacity and thermal isolation of the microfabricated column improves the thermal time response and power consumption, both important factors for portable microanalytical systems.

  2. Electrically Switched Cesium Ion Exchange

    SciTech Connect (OSTI)

    JPH Sukamto; ML Lilga; RK Orth

    1998-10-23

    This report discusses the results of work to develop Electrically Switched Ion Exchange (ESIX) for separations of ions from waste streams relevant to DOE site clean-up. ESIX combines ion exchange and electrochemistry to provide a selective, reversible method for radionuclide separation that lowers costs and minimizes secondary waste generation typically associated with conventional ion exchange. In the ESIX process, an electroactive ion exchange film is deposited onto. a high surface area electrode, and ion uptake and elution are controlled directly by modulating the potential of the film. As a result, the production of secondary waste is minimized, since the large volumes of solution associated with elution, wash, and regeneration cycles typical of standard ion exchange are not needed for the ESIX process. The document is presented in two parts: Part I, the Summary Report, discusses the objectives of the project, describes the ESIX concept and the approach taken, and summarizes the major results; Part II, the Technology Description, provides a technical description of the experimental procedures and in-depth discussions on modeling, case studies, and cost comparisons between ESIX and currently used technologies.

  3. Extraction chromatographic separation of promethium from high active waste solutions of Purex origin

    SciTech Connect (OSTI)

    Ramanujam, A.; Achuthan, P.V.; Dhami, P.S.; Gopalakrishnan, V.; Kannan, R.; Mathur, J.N. [Bhabha Atomic Research Centre, Bombay (India)

    1995-03-01

    An extraction chromatographic procedure for the separation of {sup 147}Pm from High Active Waste solutions of Purex process has been developed. Octyl(phenyl)-N,N-diisobutylcarbamoylmethylphosphine oxide(CMPO) and 2-ethylhexyl-2-ethylhexylphosphonic acid (KSM-17), both sorbed separately on an inert support(chromosorb-102) have been sequentially employed for this purpose. In the CMPO column, the rare earths and the trivalent actinides are sorbed together with uranium, plutonium and traces of few other fission products. The elution of this column with 0.04 M HNO{sub 3} gives an eluate containing trivalent actinides and lanthanides. This solution, after adjusting the pH to 2.0, is used as feed for the second extraction chromatographic column based on KSM-17. All the trivalent metal ions are sorbed on the column leaving the trace impurities in the effluent. Fractional elution of the metal ions from this column is carried out with nitric acid of varying concentrations. At 0.09 M HNO{sub 3}, the pure beta emitting fraction of {sup 147}Pm has been obtained. 16 refs., 3 figs., 2 tabs.

  4. Adsorbate shape selectivity: Separation of the HF/134a azeotrope over carbogenic molecular sieve

    SciTech Connect (OSTI)

    Hong, A.; Mariwala, R.K.; Kane, M.S.; Foley, H.C. [Univ. of Delaware, Nework, DE (United States)

    1995-03-01

    Experimental evidence is provided for adsorptive shape selectivity in the separation of the azeotrope between HF and 1,1,1,2-tetrafluoroethane (134a) over pyrolyzed poly(furfuryl alcohol)-derived carbogenic molecular sieve (PPFA-CMS). The separation can be accomplished over coconut charcoal or Carbosieve G on the basis of the differences in the extent of equilibrium adsorption of HF and 134a. On these adsorbents 134a is more strongly bound than HF, thus it elutes much more slowly from the bed. The heat of adsorption for 134a in the vicinity of 200 C on Carbosieve G is {approximately}8.8 kcal/mol. In contrast, when the same azeotropic mixture is separated over PPFA-CMS prepared at 500 C, 134a is not adsorbed. As a result 134a elutes from the bed first, followed by HF. The reversal is brought about by the narrower pore size and pore size distribution of the PPFA-CMS versus that for Carbosieve G. Thus the separation over PPFA-CMS is an example of adsorbate shape selectivity and represents a limiting case of kinetic separation.

  5. Current Status of Interventional Radiology Treatment of Infrapopliteal Arterial Disease

    SciTech Connect (OSTI)

    Rand, T., E-mail: thomas.rand@wienkav.at [General Hospital Hietzing, Department of Radiology (Austria); Uberoi, R. [John Radcliffe Hospital, Department of Radiology (United Kingdom)

    2013-06-15

    Treatment of infrapopliteal arteries has developed to a standard technique during the past two decades. With the introduction of innovative devices, a variety of techniques has been created and is still under investigation. Treatment options range from plain balloon angioplasty (POBA), all sorts of stent applications, such as bare metal, balloon expanding, self-expanding, coated and drug-eluting stents, and bio-absorbable stents, to latest developments, such as drug-eluting balloons. Regarding the scientific background, several prospective, randomized studies with relevant numbers of patients have been (or will be) published that are Level I evidence. In contrast to older studies, which primarily were based mostly on numeric parameters, such as diameters or residual stenoses, more recent study concepts focus increasingly on clinical features, such as amputation rate improvement or changes of clinical stages and quality of life standards. Although it is still not decided, which of the individual techniques might be the best one, we can definitely conclude that whatever treatment of infrapopliteal arteries will be used it is of substantial benefit for the patient. Therefore, the goal of this review is to give an overview about the current developments and techniques for the treatment of infrapopliteal arteries, to present clinical and technical results, to weigh individual techniques, and to discuss the recent developments.

  6. Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Terborg, Lydia; Masini, Jorge C.; Lin, Michelle; Lipponen, Katriina; Riekolla, Marja -Liisa; Svec, Frantisek

    2014-11-04

    A new approach has been developed for the preparation of mixed-mode stationary phases to separate proteins. The pore surface of monolithic poly(glycidyl methacrylate-co-ethylene dimethacrylate) capillary columns was functionalized with thiols and coated with gold nanoparticles. The final mixed mode surface chemistry was formed by attaching, in a single step, alkanethiols, mercaptoalkanoic acids, and their mixtures on the free surface of attached gold nanoparticles. Use of these mixtures allowed fine tuning of the hydrophobic/hydrophilic balance. The amount of attached gold nanoparticles according to thermal gravimetric analysis was 44.8 wt.%. This value together with results of frontal elution enabled calculation of surfacemore »coverage with the alkanethiol and mercaptoalkanoic acid ligands. Interestingly, alkanethiols coverage in a range of 4.46–4.51 molecules/nm2 significantly exceeded that of mercaptoalkanoic acids with 2.39–2.45 molecules/nm2. The mixed mode character of these monolithic stationary phases was for the first time demonstrated in the separations of proteins that could be achieved in the same column using gradient elution conditions typical of reverse phase (using gradient of acetonitrile in water) and ion exchange chromatographic modes (applying gradient of salt in water), respectively.« less

  7. Use of the ORNL Tungsten-188/Rhenium-188 Generator for Preparation of the Rhenium-188 HDD/Lipiodol Complex for Transarterial Liver Cancer Therapy

    SciTech Connect (OSTI)

    Knapp Jr, Russ F [ORNL; Jeong, J M [Seoul National University

    2008-01-01

    This work describes the installation, use, and quality control (QC) of the alumina-based tungsten-188 ({sup 188}W)/rhenium-188 ({sup 188}Re) generators provided by the Oak Ridge National Laboratory (ORNL). In addition, methods used for concentration of the {sup 188}Re-perrhenate bolus and preparation of {sup 188}Re-labeled HDD (4-hexadecyl-2,2,9,9-tetramethyl-4,7-diaza-1,10-decanethiol) for trans-arterial administration for therapy of nonresectable liver cancer also are described. The {sup 188}W/{sup 188}Re generator has a long useful shelf-life of several months and is a convenient on-site {sup 188}Re production system. {sup 188}Re has excellent therapeutic and imaging properties (T{sub 1/2} 16.9 hours; E{beta}{sub max} 2.12 MeV; 155-keV gamma ray, 15%) and is cost effectively obtained on demand by saline elution of the generator. The clinical efficacy of a variety of {sup 188}Re-labeled agents has been demonstrated for several therapeutic applications. Because of the favorable physical properties of {sup 188}Re, several {sup 188}Re-labeled agents are being developed and evaluated for the treatment of nonresectable/refractory liver cancer. {sup 188}Re-labeled HDD has been the most widely studied of these agents for this application and has been introduced into clinical trials at a number of institutions. The trans-arterial administration of {sup 188}Re-labeled agents for treatment of inoperable liver cancer requires use of high-level (1-2 Ci) {sup 188}W/{sup 188}Re generators. The handling of such high levels of {sup 188}Re imposes radiological precautions normally not encountered in a radiopharmacy and adequate care and ALARA (ie, 'As Low As Reasonably Achievable') principles must be followed. The ORNL generator provides consistently high {sup 188}Re yields (>75%) and low {sup 188}W parent breakthrough (<10{sup -3}%) over an extended shelf-life of several months. However, the high elution volumes (20-40 mL for 1-2 Ci generators) can require concentration of the {sup 188}Re bolus by postelution passage through silver cation chloride trapping columns used in the cost-effective tandem cation/anion column system. The silver column removes the high levels of chloride anion as insoluble AgCl, thus allowing subsequent specific trapping of the perrhenate anion on the small (QMA SeaPak) anion column. This method permits subsequent elution of {sup 188}Re-perrhenate with a small volume of saline, providing a very high activity-concentration solution. Because the {sup 188}Re-specific volume-activity concentration continually decreases with time, the tandem system is especially effective method for extending the useful generator shelf-life. Low elution flow rates (<1 mL/min) minimize any high back pressure which may be encountered during generator/tandem column elution when using tightly packed, small-particle-size commercial columns. In-house preparation of silver cation columns is recommended since the chloride trapping capacity is essentially unlimited, it is inexpensive and not limited in availability to any one supplier, and back pressure can be eliminated by the use of larger particles. Methods for the preparation of {sup 188}Re-HDD have been optimized and this agent can be obtained in high yield (80%).

  8. Use of the Oak Ridge National Laboratory tungsten-188/rhenium-188 generator for preparation of the rhenium-188 HDD/lipiodol complex for trans-arterial liver cancer therapy

    SciTech Connect (OSTI)

    Jeong, J M [Seoul National University; Knapp Jr, Russ F [ORNL

    2008-01-01

    This work describes the installation, use, and quality control (QC) of the alumina-based tungsten-188 ({sup 188}W)/rhenium-188 ({sup 188}Re) generators provided by the Oak Ridge National Laboratory (ORNL). In addition, methods used for concentration of the {sup 188}Re-perrhenate bolus and preparation of {sup 188}Re-labeled HDD (4-hexadecyl-2,2,9,9-tetramethyl-4,7-diaza-1,10-decanethiol) for trans-arterial administration for therapy of nonresectable liver cancer also are described. The {sup 188}W/{sup 188}Re generator has a long useful shelf-life of several months and is a convenient on-site {sup 188}Re production system. {sup 188}Re has excellent therapeutic and imaging properties (T{sub 1/2} 16.9 hours; E{sub {beta}max} 2.12 MeV; 155-keV gamma ray, 15%) and is cost effectively obtained on demand by saline elution of the generator. The clinical efficacy of a variety of {sup 188}Re-labeled agents has been demonstrated for several therapeutic applications. Because of the favorable physical properties of {sup 188}Re, several {sup 188}Re-labeled agents are being developed and evaluated for the treatment of nonresectable/refractory liver cancer. {sup 188}Re-labeled HDD has been the most widely studied of these agents for this application and has been introduced into clinical trials at a number of institutions. The trans-arterial administration of {sup 188}Re-labeled agents for treatment of inoperable liver cancer requires use of high-level (1-2 Ci) {sup 188}W/{sup 188}Re generators. The handling of such high levels of {sup 188}Re imposes radiological precautions normally not encountered in a radiopharmacy and adequate care and ALARA (i.e., 'As Low As Reasonably Achievable') principles must be followed. The ORNL generator provides consistently high {sup 188}Re yields (>75%) and low {sup 188}W parent breakthrough (<10{sup -3}%) over an extended shelf-life of several months. However, the high elution volumes (20-40 mL for 1-2 Ci generators) can require concentration of the {sup 188}Re bolus by postelution passage through silver cation chloride trapping columns used in the cost-effective tandem cation/anion column system. The silver column removes the high levels of chloride anion as insoluble AgCl, thus allowing subsequent specific trapping of the perrhenate anion on the small (QMA SeaPak) anion column. This method permits subsequent elution of {sup 188}Re-perrhenate with a small volume of saline, providing a very high activity-concentration solution. Because the {sup 188}Re-specific volume-activity concentration continually decreases with time, the tandem system is especially effective method for extending the useful generator shelf-life. Low elution flow rates (<1 mL/min) minimize any high back pressure which may be encountered during generator/tandem column elution when using tightly packed, small-particle-size commercial columns. In-house preparation of silver cation columns is recommended since the chloride trapping capacity is essentially unlimited, it is inexpensive and not limited in availability to any one supplier, and back pressure can be eliminated by the use of larger particles. Methods for the preparation of {sup 188}Re-HDD have been optimized and this agent can be obtained in high yield (80%).

  9. Affordable Hydrogen Fuel Cell Vehicles: Quaternary Phosphonium Based Hydroxide Exchange Membranes

    SciTech Connect (OSTI)

    2010-01-01

    Broad Funding Opportunity Announcement Project: The University of Delaware is developing a new fuel cell membrane for vehicles that relies on cheaper and more abundant materials than those used in current fuel cells. Conventional fuel cells are very acidic, so they require acid-resistant metals like platinum to generate electricity. The University of Delaware is developing an alkaline fuel cell membrane that can operate in a non-acidic environment where cheaper materials like nickel and silver, instead of platinum, can be used. In addition to enabling the use of cheaper metals, the University of Delaware’s membrane is 500 times less expensive than other polymer membranes used in conventional fuel cells.

  10. CHARACTERIZATION OF CYCLED SPHERICAL RESORCINOL-FORMALDEHYDE ION EXCHANGE RESIN

    SciTech Connect (OSTI)

    Nash, C.; Duignan, M.

    2010-02-23

    This report presents characterization data for two spherical resorcinol-formaldehyde (sRF) resin beds that had processed cesium in non-radioactive and radioactive cycles. All column cycle operations for the resin beds including loading, displacements, elution, regeneration, breakthroughs, and solution analyses are reported in Nash and Duignan, 2009a. That report covered four ion exchange (IX) campaigns using the two {approx}11 mL beds in columns in a lead-lag arrangement. The first two campaigns used Savannah River Site (SRS) Tank 2F nonradioactive simulant while the latter two were fed with actual dissolved salt in the Savannah River National Laboratory (SRNL) Shielded Cells. Both radioactive cycles ran to cesium breakthrough of the lead column. The resin beds saw in excess of 400 bed volumes of feed in each cycle. Resin disposal plans in tank farm processing depend on characterizations of resin used with actual tank feed. Following a final 30 bed volume (BV) elution with nitric acid, the resin beds were found to contain detectable chromium, barium, boron, aluminum, iron, sodium, sulfur, plutonium, cesium, and mercury. Resin affinity for plutonium is important in criticality safety considerations. Cesium-137 was found to be less than 10E+7 dpm/g of resin, similar to past work with sRF resin. Sulfur levels are reasonably consistent with other work and are expected to represent sulfur chemistry used in the resin manufacture. There were low but detectable levels of technetium, americium, and curium. Toxicity Characteristic Leaching Procedure (TCLP) work on the used (eluted) resin samples showed significant contents of mercury, barium, and chromium. One resin sample exceeded the TCLP level for mercury while the other metals were below TCLP levels. TCLP organics measurements indicated measurable benzene in one case, though the source was unknown. Results of this work were compared with other work on similar sRF resin characterizations in this report. This is the first work to quantify mercury on sRF resin. Resin mercury content is important in plans for the disposition of used sRF resin. Mercury speciation in high level waste (HLW) is unknown. It may be partly organic, one example being methyl mercury cation. Further study of the resin's affinity for mercury is recommended.

  11. Near-continuous measurement of hydrogen sulfide and carbonyl sulfide by an automatic gas chromatograph

    SciTech Connect (OSTI)

    Lindgren, E.R.; Pershing, D.W.; Kirchgessner, D.A.; Drehmel, D.C.

    1991-01-01

    The article describes an automatic gas chromatograph with a flame photometric detector (GC-FPD) that samples and analyzes hydrogen sulfide (H2S) and carbonyl sulfide (COS) at 30-sec intervals. Temperature programming was used to elute trace amounts of carbon disulfide (CS2) present in each injection from a Supelpak-S column in a single peak at the end of 15 min runs. The system was used to study the high-temperature fuel-rich sulfur capture reactions of H2S and COS with injected calcium oxide (CaO) sorbent, necessitating the near continuous measurement of these gaseous sulfur species. The H2S concentration ranged from 300 to 3000 ppm, and the COS from 30 to 300 ppm. The system was also used to monitor sulfur dioxide (SO2) levels under fuel-lean conditions: results compared very closely with SO2 measurements made simultaneously with continuous ultraviolet (UV) SO2 instrumentation.

  12. Method for the chemical separation of GE-68 from its daughter Ga-68

    DOE Patents [OSTI]

    Fitzsimmons, Jonathan M.; Atcher, Robert W.

    2010-06-01

    The present invention is directed to a generator apparatus for separating a daughter gallium-68 radioisotope substantially free of impurities from a parent gernanium-68 radioisotope, including a first resin-containing column containing parent gernanium-68 radioisotope and daughter gallium-68 radioisotope, a source of first eluent connected to said first resin-containing column for separating daughter gallium-68 radioisotope from the first resin-containing column, said first eluent including citrate whereby the separated gallium is in the form of gallium citrate, a mixing space connected to said first resin-containing column for admixing a source of hydrochloric acid with said separated gallium citrate whereby gallium citrate is converted to gallium tetrachloride, a second resin-containing column for retention of gallium-68 tetrachloride, and, a source of second eluent connected to said second resin-containing column for eluting the daughter gallium-68 radioisotope from said second resin-containing column.

  13. Method for regeneration of electroless nickel plating solution

    DOE Patents [OSTI]

    Eisenmann, E.T.

    1997-03-11

    An electroless nickel(EN)/hypophosphite plating bath is provided employing acetic acid/acetate as a buffer and which is, as a result, capable of perpetual regeneration while avoiding the production of hazardous waste. A regeneration process is provided to process the spent EN plating bath solution. A concentrated starter and replenishment solution is provided for ease of operation of the plating bath. The regeneration process employs a chelating ion exchange system to remove nickel cations from spent EN plating solution. Phosphites are then removed from the solution by precipitation. The nickel cations are removed from the ion exchange system by elution with hypophosphorus acid and the nickel concentration of the eluate adjusted by addition of nickel salt. The treated solution and adjusted eluate are combined, stabilizer added, and the volume of resulting solution reduced by evaporation to form the bath starter and replenishing solution. 1 fig.

  14. Disposable and removable nucleic acid extraction and purification cartridges for automated flow-through systems

    DOE Patents [OSTI]

    Regan, John Frederick

    2014-09-09

    Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument.

  15. Modeling of Crystalline Silicotitanate Ion Exchange Columns

    SciTech Connect (OSTI)

    Walker, D.D.

    1999-03-09

    Non-elutable ion exchange is being considered as a potential replacement for the In-Tank Precipitation process for removing cesium from Savannah River Site (SRS) radioactive waste. Crystalline silicotitanate (CST) particles are the reference ion exchange medium for the process. A major factor in the construction cost of this process is the size of the ion exchange column required to meet product specifications for decontaminated waste. To validate SRS column sizing calculations, SRS subcontracted two reknowned experts in this field to perform similar calculations: Professor R. G. Anthony, Department of Chemical Engineering, Texas A&038;M University, and Professor S. W. Wang, Department of Chemical Engineering, Purdue University. The appendices of this document contain reports from the two subcontractors. Definition of the design problem came through several meetings and conference calls between the participants and SRS personnel over the past few months. This document summarizes the problem definition and results from the two reports.

  16. Use of Chelex-100 for selectively removing Y-90 from its parent Sr-90

    DOE Patents [OSTI]

    Huntley, M.W.

    1996-02-27

    A method for selectively removing yttrium-90 from its parent strontium-90 contained in an environmental sample includes loading the sample onto a column containing a chelating ion-exchange resin capable of retaining yttrium-90; washing the column with a solution capable of removing strontium, calcium, and other contaminants from the yttrium-90 fraction retained on the column; removing excess acetate salts from the column; eluting yttrium-90 solution from the column and adjusting the pH of this solution to about 2.7; filtering the yttrium-90 solution and weighing this solution for gravimetric yield; and, counting the yttrium-90 containing solution with a radiological counter for a time sufficient to achieve the statistical accuracy desired. It is preferred that the chelating ion-exchange resin is a ligand having the chemical name iminodiacetic acid mounted on a divinyl benzene substrate, converted from sodium form to ammonia form.

  17. Use of Chelex-100 for selectively removing Y-90 from its parent Sr-90

    DOE Patents [OSTI]

    Huntley, Mark W. (Idaho Falls, ID)

    1996-01-01

    A method for selectively removing yttrium-90 from its parent strontium-90 contained in an environmental sample includes loading the sample onto a column containing a chelating ion-exchange resin capable of retaining yttrium-90; washing the column with a solution capable of removing strontium, calcium, and other contaminants from the yttrium-90 fraction retained on the column; removing excess acetate salts from the column; eluting yttrium-90 solution from the column and adjusting the pH of this solution to about 2.7; filtering the yttrium-90 solution and weighing this solution for gravimetric yield; and, counting the yttrium-90 containing solution with a radiological counter for a time sufficient to achieve the statistical accuracy desired. It is preferred that the chelating ion-exchange resin is a bidente ligand having the chemical name iminodiacetic acid mounted on a divinyl benzene substrate, converted from sodium form to ammonia form.

  18. REAL WASTE TESTING OF SPHERICAL RESORCINOL-FORMALDEHYDE ION EXCHANGE RESIN

    SciTech Connect (OSTI)

    Nash, C.; Duignan, M.

    2009-10-30

    This report presents data on batch contact and column testing tasks for spherical resorcinol-formaldehyde (sRF) resin. The testing used a non-radioactive simulant of SRS Tank 2F dissolved salt, as well as an actual radioactive waste sample of similar composition, which are both notably high in sodium (6 M). The resin was Microbeads batch 5E-370/641 which had been made on the hundred gallon scale. Equilibrium batch contact work focused on cesium at a temperature of 25 C due to the lack of such data to better benchmark existing isotherm models. Two campaigns were performed with small-scale ion exchange columns, first with Tank 2F simulant, then with actual dissolved salt in the Shielded Cells. An extrapolation of the batch contact results with radioactive waste over-predicted the cesium loaded onto the IX sRF resin bed by approximately 11%. This difference is not unexpected considering uncertainties from measurement and extrapolation and because the ion exchange that occurs when waste flows through a resin bed probably cannot reach the same level of equilibrium as when waste and resin are joined in a long term batch contact. Resin was also characterized to better understand basic chemistry issues such as holdup of trace transition metals present in the waste feed streams. The column tests involved using two beds of sRF resin in series, with the first bed referred to as the Lead column and the second bed as the Lag column. The test matrix included two complete IX cycles for both the simulant and actual waste phases. A cycle involves cesium adsorption, until the resin in the Lead column reaches saturation, and then regenerating the sRF resin, which includes eluting the cesium. Both the simulated and the actual wastes were treated with two cycles of operation, and the resin beds that were used in the Lead and Lag columns of simulant test phase were regenerated and reused in the actual waste test phase. This task is the first to demonstrate the treatment of SRS waste with sRF resin and the tests clearly demonstrated cesium decontamination for actual waste. The results of the column tests were similar for both the simulated and the actual waste and demonstrated Cs removal with sRF from both wastes. For a flowrate of 1.4 bed volumes (BV)/hour at 25 C those results with sRF resin were: (1) Simulant and actual waste results are equivalent; (2) Cs breakthrough began between 200 and 250 BV; (3) Cs breakthrough reached 100% at around 400 BV; (4) Cs breakthrough curve from 5% to 100% is approximately linear; (5) Cs elution with 0.5 M HNO3 starts at 2 BV and ends at 6BV; (6) Most, if not all, of Cs adsorbed during treatment is released during elution; (7) At 100% breakthrough of Cs the resin bed adsorbs approximately 85% of full capacity before detection in the effluent; the remaining 15% is adsorbed at saturation; (8) Approximately 90% of resin bed changes (color and volume) are complete by 6 BV; and (9) During elution the resin shrinks to about 80% of its fully working (sodium form) BV.

  19. Tanks Focus Area Alternative Salt Processing Research and Development Program Plan

    SciTech Connect (OSTI)

    Harmon, Harry D.

    2000-11-30

    In March 2000, DOE-Headquarters (HQ) requested the Tanks Focus Area (TFA) to assume management responsibility for the Salt Processing Project technology development program at Savannah River Site. The TFA was requested to conduct several activities, including review and revision of the technology development roadmaps, development of down-selection criteria, and preparation of a comprehensive Research and Development (R&D) Program Plan for three candidate cesium removal technologies, as well as the Alpha and strontium removal processes that must also be carried out. The three cesium removal candidate technologies are Crystalline Silicotitanate (CST) Non-Elutable Ion Exchange, Caustic Side Solvent Extraction (CSSX), and Small Tank Tetraphenylborate Precipitation (STTP). This plan describes the technology development needs for each process that must be satisfied in order to reach a down-selection decision, as well as continuing technology development required to support conceptual design activities.

  20. Tanks Focus Area Alternative Salt Processing Research and Development Program Plan

    SciTech Connect (OSTI)

    Harmon, Harry D.

    2000-05-15

    In March 2000, DOE-Headquarters (HQ) requested the Tanks Focus Area (TFA)to assume management responsibility for the Salt Processing Project technology development program at Savannah River Site. The TFA was requested to conduct several activities, including review and revision of the technology development roadmaps, development of down-selection criteria, and preparation of a comprehensive Research and Development (R&D) Program Plan for three candidate cesium removal technologies, as well as the Alpha and strontium removal processes that must also be carried out. The three cesium removal candidate technologies are Crystalline Silicotitanate (CST) Non-Elutable Ion Exchange, Caustic Side Solvent Extraction (CSSX), and Small Tank Tetraphenylborate Precipitation (STTP). This plan describes the technology development needs for each process that must be satisfied in order to reach a down-selection decision, as well as continuing technology development required to support conceptual design activities.

  1. Method for digesting spent ion exchange resins and recovering actinides therefrom using microwave radiation

    DOE Patents [OSTI]

    Maxwell, III, Sherrod L. (Aiken, SC); Nichols, Sheldon T. (Augusta, GA)

    1999-01-01

    The present invention relates to methods for digesting diphosphonic acid substituted cation exchange resins that have become loaded with actinides, rare earth metals, or heavy metals, in a way that allows for downstream chromatographic analysis of the adsorbed species without damage to or inadequate elution from the downstream chromatographic resins. The methods of the present invention involve contacting the loaded diphosphonic acid resin with concentrated oxidizing acid in a closed vessel, and irradiating this mixture with microwave radiation. This efficiently increases the temperature of the mixture to a level suitable for digestion of the resin without the use of dehydrating acids that can damage downstream analytical resins. In order to ensure more complete digestion, the irradiated mixture can be mixed with hydrogen peroxide or other oxidant, and reirradiated with microwave radiation.

  2. Regeneration of strong-base anion-exchange resins by sequential chemical displacement

    SciTech Connect (OSTI)

    Brown, Gilbert M.; Gu, Baohua; Moyer, Bruce A.; Bonnesen, Peter V.

    2002-01-01

    A method for regenerating strong-base anion exchange resins utilizing a sequential chemical displacement technique with new regenerant formulation. The new first regenerant solution is composed of a mixture of ferric chloride, a water-miscible organic solvent, hydrochloric acid, and water in which tetrachloroferrate anion is formed and used to displace the target anions on the resin. The second regenerant is composed of a dilute hydrochloric acid and is used to decompose tetrachloroferrate and elute ferric ions, thereby regenerating the resin. Alternative chemical displacement methods include: (1) displacement of target anions with fluoroborate followed by nitrate or salicylate and (2) displacement of target anions with salicylate followed by dilute hydrochloric acid. The methodology offers an improved regeneration efficiency, recovery, and waste minimization over the conventional displacement technique using sodium chloride (or a brine) or alkali metal hydroxide.

  3. Peripheral plasma levels of progesterone, 5?-pregnane-3,20-dione, and two monohydroxy-5?-pregnanes during late gestation in the mare 

    E-Print Network [OSTI]

    Warren, Miriam Kathleen

    1977-01-01

    decrease in P levels. Sap increased at around 80 to 50 hr prepartum in most mares to a mean high value of 133. 72 ng/ml at -80 hr and appeared to decrease at least 8 hr before the onset of parturition in all mares to a postpartum mean low value of 9. 20... 14 15 15 16 16 16 16 17 17 18 19 19 19 21 21 22 23 23 23 25 28 52 62 74 LIST OF FIGURES FIGURE PAGE Elution pattern of pregnant mare's serum compared to sH-Sap, sH-P, and H-OH5oP , 27 10 12 13 15 16 17 P and SaP serum...

  4. A rapid method for determination of beta-emitting radionuclides in aqueous samples

    SciTech Connect (OSTI)

    Reboul, S.H.; Fjeld, R.A. [Clemson Univ., SC (United States)

    1994-12-31

    Use of ion chromatography and a flow-through scintillation detector was investigated for determination of {sup 14}C, {sup 55}Fe, {sup 60}Co, {sup 63}Ni, {sup 90}Sr, {sup 90}Y, {sup 99}Tc, and {sup 147}Pm. Experiments were conducted with prepackaged elution programs and samples prepared from calibrated standards. The technique produced effective separation of the radionuclides in approximately one hour. Response of the flow-through detector was linear over the full range of activities, from 2 to 1000 Bq (50 to 30,000 pCi). Detection efficiencies ranged from 9% for {sup 63}Ni to 140% for {sup 90}Y. Lower limits of detection ranged from 5 Bq (140 pCi) for {sup 90}Y to 34 Bq (910 pCi) for {sup 63}Ni.

  5. Biomedical silver-109m isotope generator

    DOE Patents [OSTI]

    Wanek, Philip M. (Los Alamos, NM); Steinkruger, Frederick J. (Los Alamos, NM); Moody, David C. (Los Alamos, NM)

    1987-01-01

    A method, composition of matter, and apparatus for producing substantially pure Ag-109m for use in biomedical imaging techniques. Cd-109, which decays with a half-life of 453 days to Ag-109m is loaded onto an ion exchange column consisting of particulate tin phosphate. After secular equilibrium is reached in about ten minutes, Ag-109m may be selectively eluted from the column by means of a physiologically acceptable aqueous buffered eluent solution of sodium thiosulfate, and either ascorbic acid or dextrose. The breakthrough of toxic Cd-109 is on the order of 1.times.10.sup.-7, which is sufficiently low to permit administration of the Ag-109m-containing eluate, with but a minor pH adjustment, directly to a human patient within a matter of seconds.

  6. Sampling probe for microarray read out using electrospray mass spectrometry

    DOE Patents [OSTI]

    Van Berkel, Gary J.

    2004-10-12

    An automated electrospray based sampling system and method for analysis obtains samples from surface array spots having analytes. The system includes at least one probe, the probe including an inlet for flowing at least one eluting solvent to respective ones of a plurality of spots and an outlet for directing the analyte away from the spots. An automatic positioning system is provided for translating the probe relative to the spots to permit sampling of any spot. An electrospray ion source having an input fluidicly connected to the probe receives the analyte and generates ions from the analyte. The ion source provides the generated ions to a structure for analysis to identify the analyte, preferably being a mass spectrometer. The probe can be a surface contact probe, where the probe forms an enclosing seal along the periphery of the array spot surface.

  7. Non-planar microfabricated gas chromatography column

    DOE Patents [OSTI]

    Lewis, Patrick R. (Albuquerque, NM); Wheeler, David R. (Albuquerque, NM)

    2007-09-25

    A non-planar microfabricated gas chromatography column comprises a planar substrate having a plurality of through holes, a top lid and a bottom lid bonded to opposite surfaces of the planar substrate, and inlet and outlet ports for injection of a sample gas and elution of separated analytes. A plurality of such planar substrates can be aligned and stacked to provide a longer column length having a small footprint. Furthermore, two or more separate channels can enable multi-channel or multi-dimensional gas chromatography. The through holes preferably have a circular cross section and can be coated with a stationary phase material or packed with a porous packing material. Importantly, uniform stationary phase coatings can be obtained and band broadening can be minimized with the circular channels. A heating or cooling element can be disposed on at least one of the lids to enable temperature programming of the column.

  8. Fluidics platform and method for sample preparation and analysis

    DOE Patents [OSTI]

    Benner, W. Henry; Dzenitis, John M.; Bennet, William J.; Baker, Brian R.

    2014-08-19

    Herein provided are fluidics platform and method for sample preparation and analysis. The fluidics platform is capable of analyzing DNA from blood samples using amplification assays such as polymerase-chain-reaction assays and loop-mediated-isothermal-amplification assays. The fluidics platform can also be used for other types of assays and analyzes. In some embodiments, a sample in a sealed tube can be inserted directly. The following isolation, detection, and analyzes can be performed without a user's intervention. The disclosed platform may also comprises a sample preparation system with a magnetic actuator, a heater, and an air-drying mechanism, and fluid manipulation processes for extraction, washing, elution, assay assembly, assay detection, and cleaning after reactions and between samples.

  9. FINAL SUMMARIZING REPORT on Grant DE-SC0001014 "Separation of Highly Complex Mixtures by Two-dimension Liquid Chromatography"

    SciTech Connect (OSTI)

    Guiochon, Georges

    2013-09-16

    The goal of our research was a fundamental investigation of methods available for the coupling of two separate chromatographic separations that would considerably enhance the individual separation power of each of these two separations. This gain arises from the combination of two independent retention mechanisms, one of them separating the components that coelute on the other column, making possible the separation of many more compounds in a given time. The two separation mechanisms used must be very different. This is possible because many retention mechanisms are available, using different kinds of molecular interactions, hydrophobic or hydrophilic interactions, polar interactions, hydrogen bonding, complex formation, ionic interactions, steric exclusion. Two methods can be used, allowing separations to be performed in space (spreading the bands of sample components on a plate covered with stationary phase layer) or in time (eluting the sample components through a column and detecting the bands leaving the column). Both offer a wide variety of possible combinations and were studied.

  10. Biomedical silver-109m isotope generator

    DOE Patents [OSTI]

    Wanek, P.M.; Steinkruger, F.J.; Moody, D.C.

    1985-03-05

    A method, composition of matter, and apparatus for producing substantially pure Ag-109m for use in biomedical imaging techniques. Cd-109, which decays with a half-life of 453 days to Ag-109m, is loaded onto an ion exchange column consisting of particulate tin phosphate. After secular equilibrium is reached in about ten minutes, Ag-109m may be selectively eluted from the column by means of a physiologically acceptable aqueous buffered eluent solution of sodium thiosulfate, and either ascorbic acid or dextrose. The breakthrough of toxic Cd-109 is on the order of 1 x 10-7, which is sufficiently low to permit administration of the Ag-109m-containing eluate, with but a minor pH adjustment, directly to a human patient within a matter of seconds. 1 fig.

  11. Chemically modified polymeric resins for solid-phase extraction and group separation prior to analysis by liquid or gas chromatography

    SciTech Connect (OSTI)

    Schmidt, L.W.

    1993-07-01

    Polystyrene divinylbenzene was modified by acetyl, sulfonic acid, and quaternary ammonium groups. A resin functionalized with an acetyl group was impregnated in a PTFE membrane and used to extract and concentrate phenolic compounds from aqueous samples. The acetyl group created a surface easily wetted, making it an efficient adsorbent for polar compounds in water. The membrane stabilized the resin bed. Partially sulfonated high surface area resins are used to extract and group separate an aqueous mixture of neutral and basic organics; the bases are adsorbed electrostatically to the sulfonic acid groups, while the neutraons are adsorbed hydrophobically. A two-step elution is then used to separate the two fractions. A partially functionalized anion exchange resin is used to separate organic acids and phenols from neutrals in a similar way. Carboxylic acids are analyzed by HPLC and phenols by GC.

  12. Intercalation of Trichloroethene by Sediment-Associated Clay Minerals

    SciTech Connect (OSTI)

    Matthieu, Donald E.; Brusseau, Mark; Johnson, G. R.; Artiola, J. L.; Bowden, Mark E.; Curry, J. E.

    2013-01-01

    The objective of this research was to examine the potential for intercalation of trichloroethene (TCE) by clay minerals associated with aquifer sediments. Sediment samples were collected from a field site inTucson, AZ. Two widely used Montmorillonite specimen clays were employed as controls. X-ray diffraction, conducted with a controlled-environment chamber, was used to characterize smectite interlayer dspacing for three treatments (bulk air-dry sample, sample mixed with synthetic groundwater, sample mixed with TCE-saturated synthetic groundwater). The results show that the d-spacing measured for the samples treated with TCE-saturated synthetic groundwater are larger (*26%) than those of the untreated samples for all field samples as well as the specimen clays. These results indicate that TCE was intercalated by the clay minerals, which may have contributed to the extensive elution tailing observed in prior miscible-displacement experiments conducted with this sediment.

  13. Method for regeneration of electroless nickel plating solution

    DOE Patents [OSTI]

    Eisenmann, Erhard T. (5423 Vista Sandia, NE., Albuquerque, NM 87111)

    1997-01-01

    An electroless nickel(EN)/hypophosphite plating bath is provided employing acetic acid/acetate as a buffer and which is, as a result, capable of perpetual regeneration while avoiding the production of hazardous waste. A regeneration process is provided to process the spent EN plating bath solution. A concentrated starter and replenishment solution is provided for ease of operation of the plating bath. The regeneration process employs a chelating ion exchange system to remove nickel cations from spent EN plating solution. Phosphites are then removed from the solution by precipitation. The nickel cations are removed from the ion exchange system by elution with hypophosphorous acid and the nickel concentration of the eluate adjusted by addition of nickel salt. The treated solution and adjusted eluate are combined, stabilizer added, and the volume of resulting solution reduced by evaporation to form the bath starter and replenishing solution.

  14. Balloon Coating with Rapamycin Using an On-site Coating Device

    SciTech Connect (OSTI)

    Schmehl, Joerg; Ruhr, Juergen von der; Dobratz, Markus; Kehlbach, Rainer; Braun, Isabelle; Greiner, Tim-Oliver; Claussen, Claus D.; Behnisch, Boris

    2013-06-15

    Purpose. The efficacy of drug-eluting balloons has been demonstrated in clinical trials. The drug predominantly used is paclitaxel because of its lipophilic properties and the rapid onset of action. The aim of the investigation was to evaluate the feasibility and efficacy of an alternative balloon coating with rapamycin that can be applied on site.MethodsThe balloon coating (3.0/18 and 3.0/12 mm, Cathy No. 4, Translumina GmbH) with rapamycin was conducted with a coating machine (Translumina GmbH). Concentrations were 2, 2 Multiplication-Sign 2, 3, and 4 %. Measurements regarding the amount of substance released to the vessel wall were carried out on explanted porcine coronaries by means of ultraviolet and visible-light spectroscopy. Inflation time varied between 30 and 120 s. The biological effect of the coating was evaluated in a porcine peripheral overstretch and stent implantation model. Results. The amount of rapamycin on the balloon surface ranged from 558 {+-} 108 {mu}g for the 2 % solution to 1,441 {+-} 228 {mu}g in the 4 % solution. An amount of 95 {+-} 63-193 {+-} 113 {mu}g was released into the vessel wall. The quantitative measurements of the angiographic examinations 4 weeks after treatment revealed a reduction of diameter stenosis from 20.6 {+-} 17.4 % in the control group to 11.6 {+-} 5.5 % in the drug-eluting balloon group. Conclusion. A balloon coating with rapamycin omitting an excipient is possible with a dose-adjustable coating machine. However, the biological effects are moderate, which make further optimization of the coating process and evaluation of appropriate excipients necessary.

  15. LITERATURE REVIEWS TO SUPPORT ION EXCHANGE TECHNOLOGY SELECTION FOR MODULAR SALT PROCESSING

    SciTech Connect (OSTI)

    King, W

    2007-11-30

    This report summarizes the results of literature reviews conducted to support the selection of a cesium removal technology for application in a small column ion exchange (SCIX) unit supported within a high level waste tank. SCIX is being considered as a technology for the treatment of radioactive salt solutions in order to accelerate closure of waste tanks at the Savannah River Site (SRS) as part of the Modular Salt Processing (MSP) technology development program. Two ion exchange materials, spherical Resorcinol-Formaldehyde (RF) and engineered Crystalline Silicotitanate (CST), are being considered for use within the SCIX unit. Both ion exchange materials have been studied extensively and are known to have high affinities for cesium ions in caustic tank waste supernates. RF is an elutable organic resin and CST is a non-elutable inorganic material. Waste treatment processes developed for the two technologies will differ with regard to solutions processed, secondary waste streams generated, optimum column size, and waste throughput. Pertinent references, anticipated processing sequences for utilization in waste treatment, gaps in the available data, and technical comparisons will be provided for the two ion exchange materials to assist in technology selection for SCIX. The engineered, granular form of CST (UOP IE-911) was the baseline ion exchange material used for the initial development and design of the SRS SCIX process (McCabe, 2005). To date, in-tank SCIX has not been implemented for treatment of radioactive waste solutions at SRS. Since initial development and consideration of SCIX for SRS waste treatment an alternative technology has been developed as part of the River Protection Project Waste Treatment Plant (RPP-WTP) Research and Technology program (Thorson, 2006). Spherical RF resin is the baseline media for cesium removal in the RPP-WTP, which was designed for the treatment of radioactive waste supernates and is currently under construction in Hanford, WA. Application of RF for cesium removal in the Hanford WTP does not involve in-riser columns but does utilize the resin in large scale column configurations in a waste treatment facility. The basic conceptual design for SCIX involves the dissolution of saltcake in SRS Tanks 1-3 to give approximately 6 M sodium solutions and the treatment of these solutions for cesium removal using one or two columns supported within a high level waste tank. Prior to ion exchange treatment, the solutions will be filtered for removal of entrained solids. In addition to Tanks 1-3, solutions in two other tanks (37 and 41) will require treatment for cesium removal in the SCIX unit. The previous SCIX design (McCabe, 2005) utilized CST for cesium removal with downflow supernate processing and included a CST grinder following cesium loading. Grinding of CST was necessary to make the cesium-loaded material suitable for vitrification in the SRS Defense Waste Processing Facility (DWPF). Because RF resin is elutable (and reusable) and processing requires conversion between sodium and hydrogen forms using caustic and acidic solutions more liquid processing steps are involved. The WTP baseline process involves a series of caustic and acidic solutions (downflow processing) with water washes between pH transitions across neutral. In addition, due to resin swelling during conversion from hydrogen to sodium form an upflow caustic regeneration step is required. Presumably, one of these basic processes (or some variation) will be utilized for MSP for the appropriate ion exchange technology selected. CST processing involves two primary waste products: loaded CST and decontaminated salt solution (DSS). RF processing involves three primary waste products: spent RF resin, DSS, and acidic cesium eluate, although the resin is reusable and typically does not require replacement until completion of multiple treatment cycles. CST processing requires grinding of the ion exchange media, handling of solids with high cesium loading, and handling of liquid wash and conditioning solutions. RF processing requires h

  16. Chemoembolization of Hepatocellular Carcinoma with Hepasphere 30–60 ?m. Safety and Efficacy Study

    SciTech Connect (OSTI)

    Malagari, Katerina; Pomoni, Maria; Moschouris, Hippokratis; Kelekis, Alexios; Charokopakis, Angelos Bouma, Evanthia Spyridopoulos, Themistoklis; Chatziioannou, Achilles; Sotirchos, Vlasios; Karampelas, Theodoros Tamvakopoulos, Constantin; Filippiadis, Dimitrios; Karagiannis, Enangelos; Marinis, Athanasios; Koskinas, John; Kelekis, Dimitrios A.

    2013-11-22

    Background: This study examined the safety, pharmacokinetics, and efficacy of transarterial chemoembolization of hepatocellular carcinoma (HCC) using a newly developed size of a superabsorbent polymer drug-eluting embolic material.MethodsForty-five patients with documented HCC (Child–Pugh score A/B: 55.5 %/44.5 %) were embolized with HepaSphere microspheres 30–60 ?m with escalation of lesion, dose, and frequency of re-embolization. Local response was evaluated with modified response evaluation criteria in solid tumors (mRECIST). Plasma levels of doxorubicin were measured in 24 patients at baseline and at 5, 20, 40, 60, and 120 min, at 6, 24, and 48 h, and at 7 days, respectively, to determine doxorubicin in plasma (Cmax) and area under the curve (AUC). Measurements of three patients who underwent lipiodol-based conventional chemoembolization (c-TACE) were also performed.ResultsTACE with HepaSphere was well tolerated with an acceptable safety profile and no 30-day mortality. Response rates were calculated on intention-to-treat basis with complete response (CR) in 17.8 % reaching 22.2 % for the target lesion. Overall partial response (PR) was seen in 51.1 %, stable disease in 20 %, and progressive disease in 11.1 % of patients. Overall objective response (CR + PR), including patients treated at all dosages of doxorubicin, was seen in 68.9 % of cases. After a median follow-up of 15.6 months, 1-year survival is 100 %. Doxorubicin AUC was significantly lower in patients with HepaSphere 30–60 ?m (35,195 ± 27,873 ng × min/ml) than in patients with conventional TACE (103,960 ± 16,652 ng × min/ml; p = 0.009). Cmax was also significantly lower with HepaSphere 30–60 ?m (83.9 ± 32.1 ng/ml) compared with c-TACE (761.3 ± 58.8 ng/ml; p = 0.002).ConclusionHepaSphere 30–60 ?m is an effective drug-eluting embolic material with a favourable pharmacokinetic profile.

  17. The New Element Californium (Atomic Number 98)

    DOE R&D Accomplishments [OSTI]

    Seaborg, G. T.; Thompson, S. G.; Street, K. Jr.; Ghiroso, A.

    1950-06-19

    Definite identification has been made of an isotope of the element with atomic number 98 through the irradiation of Cm{sup 242} with about 35-Mev helium ions in the Berkeley Crocker Laboratory 60-inch cyclotron. The isotope which has been identified has an observed half-life of about 45 minutes and is thought to have the mass number 244. The observed mode of decay of 98{sup 244} is through the emission of alpha-particles, with energy of about 7.1 Mev, which agrees with predictions. Other considerations involving the systematics of radioactivity in this region indicate that it should also be unstable toward decay by electron capture. The chemical separation and identification of the new element was accomplished through the use of ion exchange adsorption methods employing the resin Dowex-50. The element 98 isotope appears in the eka-dysprosium position on elution curves containing berkelium and curium as reference points--that is, it precedes berkelium and curium off the column in like manner that dysprosium precedes terbium and gadolinium. The experiments so far have revealed only the tripositive oxidation state of eka-dysprosium character and suggest either that higher oxidation states are not stable in aqueous solutions or that the rates of oxidation are slow. The successful identification of so small an amount of an isotope of element 98 was possible only through having made accurate predictions of the chemical and radioactive properties.

  18. HIGH ASPECT RATIO ION EXCHANGE RESIN BED - HYDRAULIC RESULTS FOR SPERICAL RESIN BEADS

    SciTech Connect (OSTI)

    Duignan, M; Charles Nash, C; Timothy Punch, T

    2007-09-27

    A principal role of the DOE Savannah River Site is to safely dispose of a large volume of liquid nuclear waste held in many storage tanks. An in-tank ion exchange unit is being considered for cesium removal to accelerate waste processing. This unit is planned to have a relatively high bed height to diameter ratio (10:1). Complicating the design is the need to cool the ion exchange media; therefore, the ion exchange column will have a central cooling core making the flow path annular. To separate cesium from waste the media being considered is made of resorcinol formaldehyde resin deposited on spherical plastic beads and is a substitute for a previously tested resin made of crystalline silicotitanate. This spherical media not only has an advantage of being mechanically robust, but, unlike its predecessor, it is also reusable, that is, loaded cesium can be removed through elution and regeneration. Resin regeneration leads to more efficient operation and less spent resin waste, but its hydraulic performance in the planned ion exchange column was unknown. Moreover, the recycling process of this spherical resorcinol formaldehyde causes its volume to significantly shrink and swell. To determine the spherical media's hydraulic demand a linearly scaled column was designed and tested. The waste simulant used was prototypic of the wastes' viscosity and density. This paper discusses the hydraulic performance of the media that will be used to assist in the design of a full-scale unit.

  19. MODELING RESULTS FROM CESIUM ION EXCHANGE PROCESSING WITH SPHERICAL RESINS

    SciTech Connect (OSTI)

    Nash, C.; Hang, T.; Aleman, S.

    2011-01-03

    Ion exchange modeling was conducted at the Savannah River National Laboratory to compare the performance of two organic resins in support of Small Column Ion Exchange (SCIX). In-tank ion exchange (IX) columns are being considered for cesium removal at Hanford and the Savannah River Site (SRS). The spherical forms of resorcinol formaldehyde ion exchange resin (sRF) as well as a hypothetical spherical SuperLig{reg_sign} 644 (SL644) are evaluated for decontamination of dissolved saltcake wastes (supernates). Both SuperLig{reg_sign} and resorcinol formaldehyde resin beds can exhibit hydraulic problems in their granular (nonspherical) forms. SRS waste is generally lower in potassium and organic components than Hanford waste. Using VERSE-LC Version 7.8 along with the cesium Freundlich/Langmuir isotherms to simulate the waste decontamination in ion exchange columns, spherical SL644 was found to reduce column cycling by 50% for high-potassium supernates, but sRF performed equally well for the lowest-potassium feeds. Reduced cycling results in reduction of nitric acid (resin elution) and sodium addition (resin regeneration), therefore, significantly reducing life-cycle operational costs. These findings motivate the development of a spherical form of SL644. This work demonstrates the versatility of the ion exchange modeling to study the effects of resin characteristics on processing cycles, rates, and cold chemical consumption. The value of a resin with increased selectivity for cesium over potassium can be assessed for further development.

  20. Fire Safety Tests for Spherical Resorcinol Formaldehyde Resin: Data Summary Report

    SciTech Connect (OSTI)

    Kim, Dong-Sang; Peterson, Reid A.; Schweiger, Michael J.

    2012-07-30

    A draft safety evaluation of the scenario for spherical resorcinol-formaldehyde (SRF) resin fire inside the ion exchange column was performed by the Hanford Tank Waste Treatment and Immobilization Plant (WTP) Fire Safety organization. The result of this draft evaluation suggested a potential change of the fire safety classification for the Cesium Ion Exchange Process System (CXP) emergency elution vessels, equipment, and piping, which may be overly bounding based on the fire performance data from the manufacturer of the ion exchange resin selected for use at the WTP. To resolve this question, the fire properties of the SRF resin were measured by Southwest Research Institute (SwRI), following the American Society for Testing and Materials (ASTM) standard procedures, through a subcontract managed by Pacific Northwest National Laboratory (PNNL). For some tests, the ASTM standard procedures were not entirely appropriate or practical for the SRF resin material, so the procedures were modified and deviations from the ASTM standard procedures were noted. This report summarizes the results of fire safety tests performed and reported by SwRI. The efforts by PNNL were limited to summarizing the test results provided by SwRI into one consolidated data report. All as-received SwRI reports are attached to this report in the Appendix. Where applicable, the precision and bias of each test method, as given by each ASTM standard procedure, are included and compared with the SwRI test results of the SRF resin.

  1. Fire Safety Tests for Cesium-Loaded Spherical Resorcinol Formaldehyde Resin: Data Summary Report

    SciTech Connect (OSTI)

    Kim, Dong-Sang; Schweiger, Michael J.; Peterson, Reid A.

    2012-09-01

    A draft safety evaluation of the scenario for spherical resorcinol formaldehyde (SRF) resin fire inside the ion exchange column was performed by the Hanford Tank Waste Treatment and Immobilization Plant (WTP) Fire Safety organization. The result of this draft evaluation suggested a potential change of the fire safety classification for the Cesium Ion Exchange Process System (CXP) emergency elution vessels, equipment, and piping. To resolve this question, the fire properties of the SRF resin were measured by Southwest Research Institute (SwRI) through a subcontract managed by Pacific Northwest National Laboratory (PNNL). The results of initial fire safety tests on the SRF resin were documented in a previous report (WTP-RPT-218). The present report summarizes the results of additional tests performed by SwRI on the cesium-loaded SRF resin. The efforts by PNNL were limited to summarizing the test results provided by SwRI into one consolidated data report. The as-received SwRI report is attached to this report in the Appendix A. Where applicable, the precision and bias of each test method, as given by each American Society for Testing and Materials (ASTM) standard procedure, are included and compared with the SwRI test results of the cesium-loaded SRF resin.

  2. Adsorption of Ruthenium, Rhodium and Palladium from Simulated High-Level Liquid Waste by Highly Functional Xerogel - 13286

    SciTech Connect (OSTI)

    Onishi, Takashi [Fukushima Fuels and Materials Department O-arai Research and Development Center Japan Atomic Energy Agency, Narita-cho 4002, O-arai-machi, Ibaraki, 311-1393 (Japan)] [Fukushima Fuels and Materials Department O-arai Research and Development Center Japan Atomic Energy Agency, Narita-cho 4002, O-arai-machi, Ibaraki, 311-1393 (Japan); Koyama, Shin-ichi [Fukushima Fuels and Materials Department O-arai Research and Development Center Japan Atomic Energy Agency, Narita-cho 4002, O-arai-machi, Ibaraki, 311-1393 (Japan)] [Fukushima Fuels and Materials Department O-arai Research and Development Center Japan Atomic Energy Agency, Narita-cho 4002, O-arai-machi, Ibaraki, 311-1393 (Japan); Mimura, Hitoshi [Dept. of Quantum Science and Energy Engineering, Graduate School of Engineering, Tohoku University Aramaki-Aza-Aoba 6-6-01-2,Aoba-ku, Sendai-shi, Miyagi-ken, 980-8579 (Japan)] [Dept. of Quantum Science and Energy Engineering, Graduate School of Engineering, Tohoku University Aramaki-Aza-Aoba 6-6-01-2,Aoba-ku, Sendai-shi, Miyagi-ken, 980-8579 (Japan)

    2013-07-01

    Fission products are generated by fission reactions in nuclear fuel. Platinum group (Pt-G) elements, such as palladium (Pd), rhodium (Rh) and ruthenium (Ru), are also produced. Generally, Pt-G elements play important roles in chemical and electrical industries. Highly functional xerogels have been developed for recovery of these useful Pt-G elements from high - level radioactive liquid waste (HLLW). An adsorption experiment from simulated HLLW was done by the column method to study the selective adsorption of Pt-G elements, and it was found that not only Pd, Rh and Ru, but also nickel, zirconium and tellurium were adsorbed. All other elements were not adsorbed. Adsorbed Pd was recovered by washing the xerogel-packed column with thiourea solution and thiourea - nitric acid mixed solution in an elution experiment. Thiourea can be a poison for automotive exhaust emission system catalysts, so it is necessary to consider its removal. Thermal decomposition and an acid digestion treatment were conducted to remove sulfur in the recovered Pd fraction. The relative content of sulfur to Pd was decreased from 858 to 0.02 after the treatment. These results will contribute to design of the Pt-G element separation system. (authors)

  3. Coal liquefaction process streams characterization and evaluation: Application of liquid chromatographic separation methods to THF-soluble portions of integrated two-stage coal liquefaction resids

    SciTech Connect (OSTI)

    Green, J.B.; Pearson, C.D.; Young, L.L.; Green, J.A. )

    1992-05-01

    This study demonstrated the feasibility of using non-aqueous ion exchange liquid chromatography (NIELC) for the examination of the tetrahydrofuran (THF)-soluble distillation resids and THF-soluble whole oils derived from direct coal liquefaction. The technique can be used to separate the material into a number of acid, base, and neutral fractions. Each of the fractions obtained by NIELC was analyzed and then further fractionated by high-performance liquid chromatography (HPLC). The separation and analysis schemes are given in the accompanying report. With this approach, differences can be distinguished among samples obtained from different process streams in the liquefaction plant and among samples obtained at the same sampling location, but produced from different feed coals. HPLC was directly applied to one THF-soluble whole process oil without the NIELC preparation, with limited success. The direct HPLC technique used was directed toward the elution of the acid species into defined classes. The non-retained neutral and basic components of the oil were not analyzable by the direct HPLC method because of solubility limitations. Sample solubility is a major concern in the application of these techniques.

  4. Numerical estimation of adsorption energy distributions from adsorption isotherm data with the expectation-maximization method

    SciTech Connect (OSTI)

    Stanley, B.J.; Guiochon, G. |

    1993-08-01

    The expectation-maximization (EM) method of parameter estimation is used to calculate adsorption energy distributions of molecular probes from their adsorption isotherms. EM does not require prior knowledge of the distribution function or the isotherm, requires no smoothing of the isotherm data, and converges with high stability towards the maximum-likelihood estimate. The method is therefore robust and accurate at high iteration numbers. The EM algorithm is tested with simulated energy distributions corresponding to unimodal Gaussian, bimodal Gaussian, Poisson distributions, and the distributions resulting from Misra isotherms. Theoretical isotherms are generated from these distributions using the Langmuir model, and then chromatographic band profiles are computed using the ideal model of chromatography. Noise is then introduced in the theoretical band profiles comparable to those observed experimentally. The isotherm is then calculated using the elution-by-characteristic points method. The energy distribution given by the EM method is compared to the original one. Results are contrasted to those obtained with the House and Jaycock algorithm HILDA, and shown to be superior in terms of robustness, accuracy, and information theory. The effect of undersampling of the high-pressure/low-energy region of the adsorption is reported and discussed for the EM algorithm, as well as the effect of signal-to-noise ratio on the degree of heterogeneity that may be estimated experimentally.

  5. Separation Of Uranium And Plutonium Isotopes For Measurement By Multi Collector Inductively Coupled Plasma Mass Spectroscopy

    SciTech Connect (OSTI)

    Martinelli, R E; Hamilton, T F; Williams, R W; Kehl, S R

    2009-03-29

    Uranium (U) and plutonium (Pu) isotopes in coral soils, contaminated by nuclear weapons testing in the northern Marshall Islands, were isolated by ion-exchange chromatography and analyzed by mass spectrometry. The soil samples were spiked with {sup 233}U and {sup 242}Pu tracers, dissolved in minerals acids, and U and Pu isotopes isolated and purified on commercially available ion-exchange columns. The ion-exchange technique employed a TEVA{reg_sign} column coupled to a UTEVA{reg_sign} column. U and Pu isotope fractions were then further isolated using separate elution schemes, and the purified fractions containing U and Pu isotopes analyzed sequentially using multi-collector inductively coupled plasma mass spectrometer (MCICP-MS). High precision measurements of {sup 234}U/{sup 235}U, {sup 238}U/{sup 235}U, {sup 236}U/{sup 235}U, and {sup 240}Pu/{sup 239}Pu in soil samples were attained using the described methodology and instrumentation, and provide a basis for conducting more detailed assessments of the behavior and transfer of uranium and plutonium in the environment.

  6. Role of the endopeptidase 24. 11 in the disposition and metabolism of endogenous atrial natriuretic factor in the rabbit

    SciTech Connect (OSTI)

    Marleau, S.; Nguyen, T.T.; Du Souich, P.; Bellemare, M.; De Lean, A.; Ong, H. (Universite de Montreal, Quebec (Canada))

    1991-01-01

    The neutral endopeptidase 24.11 (NEP) has been shown to inactivate the atrial natriuretic factor (ANF) by opening the ring structure. To document the role of NEP in the metabolic fate of ANF in vivo, the effects of an infusion of thiorphan (25 micrograms/min/kg), a specific NEP inhibitor, on the kinetics and metabolism of endogenous ANF were studied in conscious rabbits. A bolus of ({sup 125}I)ANF(99-126) was injected 50 min after the beginning of the infusion of thiorphan. Plasma samples containing the radioactive peptides were separated by reverse-phase HPLC. The parent compound could be separated from at least two other minor metabolites, corresponding to the elution position of ({sup 125}I)ANF(99-105/106-126), the inactive ring-opened metabolite, and of ({sup 125}I)ANF(103-126), an N-truncated analog. The generation of the N-truncated metabolite was increased by thiorphan. Thiorphan also induced an increase in plasma ANF (29%) that was closely associated with a 32% reduction in the systemic clearance of ({sup 125}I)ANF(99-126), whereas no modification in the estimated secretion rate was detected. These results support a role for NEP in the regulation of endogenous ANF plasma levels. These results also suggest that specific inhibition of NEP may result in an increase in the apparent activity of alternative metabolic pathways.

  7. Four methods for determining the composition of trace radioactive surface contamination of low-radioactivity metal

    E-Print Network [OSTI]

    H. M. O'Keeffe; T. H. Burritt; B. T. Cleveland; G. Doucas; N. Gagnon; N. A. Jelley; C. Kraus; I. T. Lawson; S. Majerus; S. R. McGee; A. W. Myers; A. W. P. Poon; K. Rielage; R. G. H. Robertson; R. C. Rosten; L. C. Stonehill; B. A. VanDevender; T. D. Van Wechel

    2011-03-29

    Four methods for determining the composition of low-level uranium- and thorium-chain surface contamination are presented. One method is the observation of Cherenkov light production in water. In two additional methods a position-sensitive proportional counter surrounding the surface is used to make both a measurement of the energy spectrum of alpha particle emissions and also coincidence measurements to derive the thorium-chain content based on the presence of short-lived isotopes in that decay chain. The fourth method is a radiochemical technique in which the surface is eluted with a weak acid, the eluate is concentrated, added to liquid scintillator and assayed by recording beta-alpha coincidences. These methods were used to characterize two `hotspots' on the outer surface of one of the He-3 proportional counters in the Neutral Current Detection array of the Sudbury Neutrino Observatory experiment. The methods have similar sensitivities, of order tens of ng, to both thorium- and uranium-chain contamination.

  8. Continuous countercurrent chromatographic separator for the purification of sugars from biomass hydrolyzate. Final project report, July 1, 1996--September 30, 1997

    SciTech Connect (OSTI)

    Wooley, R.J.

    1997-12-01

    Production of pure sugars is required to enable production of fuels and chemicals from biomass feedstocks. Hydrolysis of cellulose and hemicellulose (principal constituents of biomass) produces sugars that can be utilized in various fermentation process to produce valuable chemicals. Unfortunately, the hydrolysis process also liberates chemicals from the biomass that can be toxic to the fermenting organisms. The two primary toxic components of biomass hydrolyzate are sulfuric acid (catalyst used in the hydrolysis) and acetic acid (a component of the feed biomass). In the standard batch chromatographic separation of these three components, sugar elutes in the middle. Batch chromatographic separations are not practical on a commercial scale, because of excess dilution and high capital costs. Because sugar is the {open_quotes}center product,{close_quotes} a continuous separation would require two costly binary separators. However, a single, slightly larger separator, configured to produce three products, would be more economical. This FIRST project develops a cost-effective method for purifying biomass hydrolyzate into fermentable sugars using a single continuous countercurrent separator to separate this ternary mixture.

  9. Uptake of metal ions by extraction chromatography using dimethyl dibutyl tetradecyl-1,3-malonamide (DMDBTDMA) as the stationary phase

    SciTech Connect (OSTI)

    Mohapatra, P.K.; Sriram, S.; Manchanda, V.K.; Badheka, L.P.

    2000-01-01

    The uptake of several actinides and fission products such as U(VI), Pu(IV), Am(III), Eu(III), Cs(I), and Sr(II) from nitric acid solutions by a novel extraction chromatographic resin containing dimethyl dibutyl tetradecyl-1,3-malonamide (DMDBTDMA) has been investigated. the order of uptake follows the order Pu(IV) > U(VI) {much{underscore}gt} AM(III) {approximately} Eu(III) > Sr(II) {approximately} Cs(I). The possibility of this resin material sorbing trace concentrations of actinide ions from nitric acid solutions containing relatively larger amounts of Nd(III) and U(VI), as well as from simulated pressurized heavy water reactor (PHWR) waste solution, has been evaluated. The uptake of Am(III) in the presence of NaNO{sub 3}, as well as in the presence of a macro concentration of Fe(III), has been investigated. The capacity of the resin for Am(III) and the elution behavior of the actinide ions from the resin were also studied.

  10. Rapid separation and purification of uranium and plutonium from dilute-matrix samples

    SciTech Connect (OSTI)

    Armstrong, Christopher R.; Ticknor, Brian W.; Hall, Gregory; Cadieux, James R.

    2014-03-11

    This work presents a streamlined separation and purification approach for trace uranium and plutonium from dilute (carrier-free) matrices. The method, effective for nanogram quantities of U and femtogram to picogram quantities of Pu, is ideally suited for environmental swipe samples that contain a small amount of collected bulk material. As such, it may be applicable for processing swipe samples such as those collected in IAEA inspection activities as well as swipes that are loaded with unknown analytes, such as those implemented in interlaboratory round-robin or proficiency tests. Additionally, the simplified actinide separation could find use in internal laboratory monitoring of clean room conditions prior to or following more extensive chemical processing. We describe key modifications to conventional techniques that result in a relatively rapid, cost-effective, and efficient U and Pu separation process. We demonstrate the efficacy of implementing anion exchange chromatography in a single column approach. We also show that hydrobromic acid is an effective substitute in lieu of hydroiodoic acid for eluting Pu. Lastly, we show that nitric acid is an effective digestion agent in lieu of perchloric acid and/or hydrofluoric acid. A step by step procedure of this process is detailed.

  11. Literature Review of Spherical Resorcinol-Formaldehyde for Cesium Ion Exchange

    SciTech Connect (OSTI)

    Brown, Garrett N.

    2014-09-30

    The current report summarizes work performed throughout the scientific community and DOE complex as reported in the open literature and DOE-sponsored reports to evaluate the Cs+ ion exchange (CIX) characteristics of SRF resin. King (2007) completed a similar literature review in support of material selection for the Small Column Ion Exchange (SCIX) project. Josephson et al. (2010) and Sams et al. (2009) provided a similar brief review of SRF CIX for the near-tank Cs+ removal (NTCR) project. Thorson (2008a) documented the basis for recommending SRF over SuperLigTM 644 as the primary CIX resin in the WTP. The current review expands on previous work, summarizes additional work completed to date, and provides a broad view of the literature without focusing on a specific column system. Although the focus of the current review is the SRF resin, many cited references include multiple materials such as the non-spherical GGRF and SuperLigTM 644 organic resins and crystalline silicotitanate (CST) IONSIVTM IE-911, a non-elutable inorganic material. This report summarizes relevant information provided in the literature.

  12. A Serendipitous, Long-Term Infiltration Experiment: Water and Tritium Circulation Beneath the CAMBRIC Ditch at the Nevada Test Site

    SciTech Connect (OSTI)

    Maxwell, R M; Tompson, A B; Kollet, S J

    2008-11-20

    Underground nuclear weapons testing at the Nevada Test Site introduced numerous radionuclides that may be used to characterize subsurface hydrologic transport processes in arid climates. A sixteen year pumping experiment designed to examine radionuclide migration away from the CAMBRIC nuclear test, conducted in groundwater beneath Frenchman Flat in 1965, gave rise to an unintended second experiment involving radionuclide infiltration through the vadose zone, as induced by seepage of pumping effluents beneath an unlined discharge trench. The combined experiments have been reanalyzed using a detailed, three-dimensional numerical model of transient, variably saturated flow and mass transport, tailored specifically for large scale and efficient calculations. Simulations have been used to estimate radionuclide travel and residence times in various parts of the system for comparison with observations in wells. Model predictions of mass transport were able to clearly demonstrate radionuclide recycling behavior between the ditch and pumping well previously suggested by isotopic age dating information; match travel time estimates for radionuclides moving between the ditch, the water table, and monitoring wells; and provide more realistic ways in which to interpret the pumping well elution curves. Collectively, the results illustrate the utility of integrating detailed numerical modeling with diverse observational data in developing accurate interpretations and forecasts of contaminant migration processes.

  13. Review of information on ferrocyanide solids for removal of cesium from solutions

    SciTech Connect (OSTI)

    Haas, P.A. (Oak Ridge National Lab., TN (United States))

    1993-12-01

    Ferrocyanide solids have important applications to the removal of radioactive cesium from nuclear waste solutions. These materials are prepared by mixing soluble ferrocyanides and salts of divalent transition metals or other divalent cations. The simple precipitations most commonly give very fine particles or slimes of variable compositions. Special preparation procedures have been developed to control the compositions or to prepare granular solids suitable for column operation. The removal of cesium from solutions has been measured for many different ferrocyanide solids. Some of these solids show an exchange of K[sup +], Na[sup +], or NH[sub 4][sup +] for cesium, but many show sorptions of cesium without a true ion exchange. The performance for cesium removal is described by measurements of the distribution coefficients for cesium with large excesses of ferrocyanides, the capacity for cesium with excess cesium in solution, and the rates of cesium removal. The chemical and physical stability, the solubility, and the elution or recovery requirements for ferrocyanide solids are important to practical applications. These properties are reviewed along with several of the proposed applications. 53 refs., 3 figs., 3 tabs.

  14. Spiral wound extraction cartridge

    DOE Patents [OSTI]

    Wisted, E.E.; Lundquist, S.H.

    1999-04-27

    A cartridge device for removing an analyte from a fluid comprises a hollow core, a sheet composite comprising a particulate-loaded porous membrane and optionally at least one reinforcing spacer sheet, the particulate being capable of binding the analyte, the sheet composite being formed into a spiral configuration about the core, wherein the sheet composite is wound around itself and wherein the windings of sheet composite are of sufficient tightness so that adjacent layers are essentially free of spaces therebetween, two end caps which are disposed over the core and the lateral ends of the spirally wound sheet composite, and means for securing the end caps to the core, the end caps also being secured to the lateral ends of the spirally wound sheet composite. A method for removing an analyte from a fluid comprises the steps of providing a spirally wound element of the invention and passing the fluid containing the analyte through the element essentially normal to a surface of the sheet composite so as to bind the analyte to the particulate of the particulate-loaded porous membrane, the method optionally including the step of eluting the bound analyte from the sheet composite. 4 figs.

  15. Spiral wound extraction cartridge

    DOE Patents [OSTI]

    Wisted, Eric E. (Apple Valley, MN); Lundquist, Susan H. (White Bear Township, MN)

    1999-01-01

    A cartridge device for removing an analyte from a fluid comprises a hollow core, a sheet composite comprising a particulate-loaded porous membrane and optionally at least one reinforcing spacer sheet, the particulate being capable of binding the analyte, the sheet composite being formed into a spiral configuration about the core, wherein the sheet composite is wound around itself and wherein the windings of sheet composite are of sufficient tightness so that adjacent layers are essentially free of spaces therebetween, two end caps which are disposed over the core and the lateral ends of the spirally wound sheet composite, and means for securing the end caps to the core, the end caps also being secured to the lateral ends of the spirally wound sheet composite. A method for removing an analyte from a fluid comprises the steps of providing a spirally wound element of the invention and passing the fluid containing the analyte through the element essentially normal to a surface of the sheet composite so as to bind the analyte to the particulate of the particulate-loaded porous membrane, the method optionally including the step of eluting the bound analyte from the sheet composite.

  16. Upscaling of U(VI) Desorption and Transport from Decimeter-Scale Heterogeneity to Plume-Scale Modeling

    SciTech Connect (OSTI)

    Curtis, Gary P; Kohler, Matthias; Kannappan, Ramakrishnan; Briggs, Martin; Day-Lewis, Fred

    2015-02-24

    Reactive solute transport in aquifers is commonly affected by rate limited mass transfer. This slow mass transfer can exhibit significant control on the times required to restore contaminated aquifers to near-pristine conditions under both ambient and forced-gradient flow systems and is therefore important to understand. Both nonreactive and reactive tracer experiments provide valuable insight into the exchange of solute between mobile and immobile porosity. At the grain scale and column scale, mass transfer limitations were manifested as a concentration rebound when contaminated sediments were contacted with pristine groundwater. This behavior was successfully modeled using the multirate mass transfer model. Mass transfer observed in a 2 m long intermediate laboratory scale experiment showed significant concentration rebound in the first half meter along a flowpath through the tank and negligible rebound near the exit of the tank. Experimental observations and model simulations show that although concentration rebound was small at the end of the tank, the overall elution of uranium from of the tank was still controlled by mass transfer which was manifested by a long tail. At the field scale, mass transfer parameters inferred from geo-electrical measurements of bulk conductivity and traditional conductivity measurements of fluid samples showed significant spatial variability. Overall the improved understanding of mass transfer across multiple scales should lead to more robust reactive transport simulations and site management.

  17. Membrane associated phospholipase C from bovine brain

    SciTech Connect (OSTI)

    Lee, K.; Ryu, S.H.; Suh, P.; Choi, W.C.; Rhee, S.G.

    1987-05-01

    Cytosolic fractions of bovine brain contain 2 immunologically distinct phosphoinositide-specific phospholipase (PLC), PLC-I and PLC-II, whose MW are 150,000 and 145,000 respectively, under a denaturing condition. Monoclonal antibodies were derived against each form and specific radioimmunoassays were developed. Distribution of PLC-I and PLC-II in cytosolic and particulate fractions was measured using the radioimmunoassay. More than 90% of PLC-II was found in the cytosolic fraction, while the anti-PLC-I antibody cross-reacting protein was distributed nearly equally between the soluble fraction and the 2 M KCl extract of particulate fraction. The PLC enzyme in the particulate fraction was purified to homogeneity, yielding 2 proteins of 140 KDa and 150 KDa when analyzed on SDS-PAGE. Neither of the 2 enzymes cross-reacted with anti-PLC-II antibodies, but both could be immunoblotted by all 4 different anti-PLC-I antibodies. This suggests that the 140 KDa PLC was derived from the 150 KDa form. The 150 Kda form from particulate fraction was indistinguishable from the cytosolic PLC-I when their mixture was analyzed on SDS-PAGE. In addition, the elution profile of tryptic peptides derived from the 150 KDa particulate form was identical to that of cytosolic PLC-I. This result indicates that PLC-I is reversibly associated to membranes.

  18. Rapid separation and purification of uranium and plutonium from dilute-matrix samples

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Armstrong, Christopher R.; Ticknor, Brian W.; Hall, Gregory; Cadieux, James R.

    2014-03-11

    This work presents a streamlined separation and purification approach for trace uranium and plutonium from dilute (carrier-free) matrices. The method, effective for nanogram quantities of U and femtogram to picogram quantities of Pu, is ideally suited for environmental swipe samples that contain a small amount of collected bulk material. As such, it may be applicable for processing swipe samples such as those collected in IAEA inspection activities as well as swipes that are loaded with unknown analytes, such as those implemented in interlaboratory round-robin or proficiency tests. Additionally, the simplified actinide separation could find use in internal laboratory monitoring ofmore »clean room conditions prior to or following more extensive chemical processing. We describe key modifications to conventional techniques that result in a relatively rapid, cost-effective, and efficient U and Pu separation process. We demonstrate the efficacy of implementing anion exchange chromatography in a single column approach. We also show that hydrobromic acid is an effective substitute in lieu of hydroiodoic acid for eluting Pu. Lastly, we show that nitric acid is an effective digestion agent in lieu of perchloric acid and/or hydrofluoric acid. A step by step procedure of this process is detailed.« less

  19. The peanut lectin-binding glycoproteins of human epidermal keratinocytes

    SciTech Connect (OSTI)

    Morrison, A.I. (Max-Planck-Institute fuer Systemphysiologie, Dortmund (West Germany)); Keeble, S.; Watt, F.M. (Imperial Cancer Research Fund, London (England))

    1988-08-01

    The peanut lectin (PNA) is known to bind more strongly to keratinocytes that are undergoing terminal differentiation than to proliferating keratinocytes. In order to investigate the significance of this change in cell-surface carbohydrate authors have identified the PNA-binding glycoproteins of cultured human keratinocytes and antibodies against them. Two heavily glycosylated bands of 110 and 250 kDa were resolved by PAGE of ({sup 14}C)galactose- or ({sup 14}C)mannose- and ({sup 14}C)glucosamine-labeled cell extracts eluted with galactose from PNA affinity columns. The higher molecular weight band was also detected on PNA blots of unlabeled cell extracts transferred to nitrocellulose. Both bands were sensitive to pronase digestion, but only the 250-kDa band was digested with trypsin. A rabbit antiserum that we prepared (anti-PNA-gp) immunoprecipitated both bands from cell extracts. In contrast to PNA, anti-PNA-gp bound equally to proliferating and terminally differentiating cells, indicating that some epitope(s) of the PNA-binding glycoproteins is present on the cell surface prior to terminal differentiation. When keratinocytes grown as a monolayer in low-calcium medium were switched to medium containing 2 mM calcium ions in order to induce desmosome formation and stratification, there was a dramatic redistribution of the PNA-binding glycoproteins, which became concentrated at the boundaries between cells. This may suggest a role for the glycoproteins in cell-cell interactions during stratification.

  20. Ultratrace detector for hand-held gas chromatography

    DOE Patents [OSTI]

    Andresen, Brian D. (Livermore, CA); Miller, Fred S. (Bethal Island, CA)

    1999-01-01

    An ultratrace detector system for hand-held gas chromatography having high sensitivity, for example, to emissions generated during production of weapons, biological compounds, drugs, etc. The detector system is insensitive to water, air, helium, argon, oxygen, and C0.sub.2. The detector system is basically composed of a hand-held capillary gas chromatography (GC), an insulated heated redox-chamber, a detection chamber, and a vapor trap. For example, the detector system may use gas phase redox reactions and spectral absorption of mercury vapor. The gas chromatograph initially separates compounds that percolate through a bed of heated mercuric oxide (HgO) in a silica--or other metal--aerogel material which acts as an insulator. Compounds easily oxidized by HgO liberate atomic mercury that subsequently pass through a detection chamber which includes a detector cell, such as quartz, that is illuminated with a 254 nm ultra-violet (UV) mercury discharge lamp which generates the exact mercury absorption bands that are used to detect the liberated mercury atoms. Atomic mercury strongly absorbs 254 nm energy is therefore a specific signal for reducing compounds eluting from the capillary GC, whereafter the atomic mercury is trapped for example, in a silicon-aerogel trap.

  1. Method for detection of extremely low concentration

    DOE Patents [OSTI]

    Andresen, Brian D. (Livermore, CA); Miller, Fred S. (Bethal Island, CA)

    2002-01-01

    An ultratrace detector system for hand-held gas chromatography having high sensitivity, for example, to emissions generated during production of weapons, biological compounds, drugs, etc. The detector system is insensitive to water, air, helium, argon, oxygen, and CO.sub.2. The detector system is basically composed of a hand-held capillary gas chromatography (GC), an insulated heated redox-chamber, a detection chamber, and a vapor trap. For example, the detector system may use gas phase redox reactions and spectral absorption of mercury vapor. The gas chromatograph initially separates compounds that percolate through a bed of heated mercuric oxide (HgO) in a silica--or other metal--aerogel material which acts as an insulator. Compounds easily oxidized by HgO liberate atomic mercury that subsequently pass through a detection chamber which includes a detector cell, such as quartz, that is illuminated with a 254 nm ultra-violet (UV) mercury discharge lamp which generates the exact mercury absorption bands that are used to detect the liberated mercury atoms. Atomic mercury strongly absorbs 254 nm energy is therefore a specific signal for reducing compounds eluting from the capillary GC, whereafter the atomic mercury is trapped for example, in a silicon-aerogel trap.

  2. Radiant{trademark} Liquid Radioisotope Intravascular Radiation Therapy System

    SciTech Connect (OSTI)

    Eigler, N.; Whiting, J.; Chernomorsky, A.; Jackson, J.; Knapp, F.F., Jr.; Litvack, F.

    1998-01-16

    RADIANT{trademark} is manufactured by United States Surgical Corporation, Vascular Therapies Division, (formerly Progressive Angioplasty Systems). The system comprises a liquid {beta}-radiation source, a shielded isolation/transfer device (ISAT), modified over-the-wire or rapid exchange delivery balloons, and accessory kits. The liquid {beta}-source is Rhenium-188 in the form of sodium perrhenate (NaReO{sub 4}), Rhenium-188 is primarily a {beta}-emitter with a physical half-life of 17.0 hours. The maximum energy of the {beta}-particles is 2.1 MeV. The source is produced daily in the nuclear pharmacy hot lab by eluting a Tungsten-188/Rhenium-188 generator manufactured by Oak Ridge National Laboratory (ORNL). Using anion exchange columns and Millipore filters the effluent is concentrated to approximately 100 mCi/ml, calibrated, and loaded into the (ISAT) which is subsequently transported to the cardiac catheterization laboratory. The delivery catheters are modified Champion{trademark} over-the-wire, and TNT{trademark} rapid exchange stent delivery balloons. These balloons have thickened polyethylene walls to augment puncture resistance; dual radio-opaque markers and specially configured connectors.

  3. Effect of force-induced mechanical stress at the coronary artery bifurcation stenting: Relation to in-stent restenosis

    SciTech Connect (OSTI)

    Lee, Cheng-Hung [Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan (China); Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan (China); Jhong, Guan-Heng [Graduate Institute of Medical Mechatronics, Chang Gung University, Tao-Yuan, Taiwan (China); Hsu, Ming-Yi; Wang, Chao-Jan [Department of Medical Imaging and Intervention, Chang Gung Memorial Hospital, Linkou, Tao-Yuan, Taiwan (China); Liu, Shih-Jung, E-mail: shihjung@mail.cgu.edu.tw [Department of Mechanical Engineering, Chang Gung University, Tao-Yuan, Taiwan (China); Hung, Kuo-Chun [Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital, Linkou, Chang Gung University College of Medicine, Tao-Yuan, Taiwan (China)

    2014-05-28

    The deployment of metallic stents during percutaneous coronary intervention has become common in the treatment of coronary bifurcation lesions. However, restenosis occurs mostly at the bifurcation area even in present era of drug-eluting stents. To achieve adequate deployment, physicians may unintentionally apply force to the strut of the stents through balloon, guiding catheters, or other devices. This force may deform the struts and impose excessive mechanical stresses on the arterial vessels, resulting in detrimental outcomes. This study investigated the relationship between the distribution of stress in a stent and bifurcation angle using finite element analysis. The unintentionally applied force following stent implantation was measured using a force sensor that was made in the laboratory. Geometrical information on the coronary arteries of 11 subjects was extracted from contrast-enhanced computed tomography scan data. The numerical results reveal that the application of force by physicians generated significantly higher mechanical stresses in the arterial bifurcation than in the proximal and distal parts of the stent (post hoc P?

  4. Method for selective recovery of PET-usable quantities of [.sup.18 F] fluoride and [.sup.13 N] nitrate/nitrite from a single irradiation of low-enriched [.sup.18 O] water

    DOE Patents [OSTI]

    Ferrieri, Richard A. (Patchogue, NY); Schlyer, David J. (Bellport, NY); Shea, Colleen (Wading River, NY)

    1995-06-13

    A process for simultaneously producing PET-usable quantities of [.sup.13 N]NH.sub.3 and [.sup.18 F]F.sup.- for radiotracer synthesis is disclosed. The process includes producing [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- and [.sup.18 F]F.sup.- simultaneously by exposing a low-enriched (20%-30%) [.sup.18 O]H.sub.2 O target to proton irradiation, sequentially isolating the [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- and [.sup.18 F]F.sup.- from the [.sup.18 O]H.sub.2 O target, and reducing the [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- to [.sup.13 N]NH.sub.3. The [.sup.13 N]NH.sub.3 and [.sup.18 F]F.sup.- products are then conveyed to a laboratory for radiotracer applications. The process employs an anion exchange resin for isolation of the isotopes from the [.sup.18 O]H.sub.2 O, and sequential elution of [.sup.13 N]NO.sub.2.sup.- /NO.sub.3.sup.- and [ .sup.18 F]F.sup.- fractions. Also the apparatus is disclosed for simultaneously producing PET-usable quantities of [.sup.13 N]NH.sub.3 and [.sup.18 F]F.sup.- from a single irradiation of a single low-enriched [.sup.18 O]H.sub.2 O target.

  5. Identification and characterization of conservative organic tracers for use as hydrologic tracers for the Yucca Mountain site characterization study. Progress report, October 1, 1994--December 31, 1994

    SciTech Connect (OSTI)

    Stetzenbach, K.; Farnham, I.

    1994-12-31

    The bromide anion has been used extensively as a tracer for mapping the flow of groundwater. It has proven to be both a safe and reliable groundwater tracer. The goal in this study is to find several tracing compounds with characteristics similar to the bromide anion to be used in multiple well tracing tests. Four groups of fluorinated organic acids were selected as candidates for groundwater tracers. These groups include fluorinated benzoic acids (FBA), fluorinated salicylic acids (FSA), fluorinated toluic acids (FTA), and fluorinated cinnamic acids (FCA). These compounds have been shown to move readily with the flow of water and do not adsorb to soil. They are also non-toxic. In this study, the retention of the fluorinated organic acids on to a soil column is compared to that of the bromide ion. The time required for the elution of each analyte from the soil column is measured using a UV-Vis detector. The soils consist of the light, medium, and dark tuffs used in the batch study. The work performed during this quarter consists of the continuation of the batch studies for the fluorinated benzoic acids and column studies for several potential tracer compounds.

  6. Detection of high molecular weight organic tracers in vegetation smoke samples by high-temperature gas chromatography-mass spectrometry

    SciTech Connect (OSTI)

    Elias, V.O.; Simoneit, B.R.T. ); Pereira, A.S.; Cardoso, J.N. ); Cabral, J.A. )

    1999-07-15

    High-temperature high-resolution gas chromatography (HTGC) is an established technique for the separation of complex mixtures of high molecular weight (HMW) compounds which do not elute when analyzed on conventional GC columns. The combination of this technique with mass spectrometry is not so common and application to aerosols is novel. The HTGC and HTGC-MS analyses of smoke samples taken by particle filtration from combustion of different species of plants provided the characterization of various classes of HMW compounds reported to occur for the first time in emissions from biomass burning. Among these components are a series of wax esters with up to 58 carbon numbers, aliphatic hydrocarbons, triglycerides, long chain methyl ketones, alkanols and a series of triterpenyl fatty acid esters which have been characterized as novel natural products. Long chain fatty acids with more than 32 carbon numbers are not present in the smoke samples analyzed. The HMW compounds in smoke samples from the burning of plants from Amazonia indicate the input of directly volatilized natural products in the original plants during their combustion. However, the major organic compounds extracted from smoke consist of a series of lower molecular weight polar components, which are not natural products but the result of the thermal breakdown of cellulose and lignin. In contrast, the HMW natural products may be suitable tracers for specific sources of vegetation combustion because they are emitted as particles without thermal alternation in the smoke and can thus be related directly to the original plant material.

  7. Development of a high-throughput microfluidic integrated microarray for the detection of chimeric bioweapons.

    SciTech Connect (OSTI)

    Sheppod, Timothy; Satterfield, Brent; Hukari, Kyle W.; West, Jason A. A.; Hux, Gary A.

    2006-10-01

    The advancement of DNA cloning has significantly augmented the potential threat of a focused bioweapon assault, such as a terrorist attack. With current DNA cloning techniques, toxin genes from the most dangerous (but environmentally labile) bacterial or viral organism can now be selected and inserted into robust organism to produce an infinite number of deadly chimeric bioweapons. In order to neutralize such a threat, accurate detection of the expressed toxin genes, rather than classification on strain or genealogical decent of these organisms, is critical. The development of a high-throughput microarray approach will enable the detection of unknowns chimeric bioweapons. The development of a high-throughput microarray approach will enable the detection of unknown bioweapons. We have developed a unique microfluidic approach to capture and concentrate these threat genes (mRNA's) upto a 30 fold concentration. These captured oligonucleotides can then be used to synthesize in situ oligonucleotide copies (cDNA probes) of the captured genes. An integrated microfluidic architecture will enable us to control flows of reagents, perform clean-up steps and finally elute nanoliter volumes of synthesized oligonucleotides probes. The integrated approach has enabled a process where chimeric or conventional bioweapons can rapidly be identified based on their toxic function, rather than being restricted to information that may not identify the critical nature of the threat.

  8. Recent International R&D Activities in the Extraction of Uranium from Seawater

    SciTech Connect (OSTI)

    Rao, Linfeng

    2010-03-15

    A literature survey has been conducted to collect information on the International R&D activities in the extraction of uranium from seawater for the period from the 1960s till the year of 2010. The reported activities, on both the laboratory scale bench experiments and the large scale marine experiments, were summarized by country/region in this report. Among all countries where such activities have been reported, Japan has carried out the most advanced large scale marine experiments with the amidoxime-based system, and achieved the collection efficiency (1.5 g-U/kg-adsorbent for 30 days soaking in the ocean) that could justify the development of industrial scale marine systems to produce uranium from seawater at the price competitive with those from conventional uranium resources. R&D opportunities are discussed for improving the system performance (selectivity for uranium, loading capacity, chemical stability and mechanical durability in the sorption-elution cycle, and sorption kinetics) and making the collection of uranium from seawater more economically competitive.

  9. Highly Efficient siRNA Delivery from Core-Shell Mesoporous Silica Nanoparticles with Multifunctional Polymer Caps

    E-Print Network [OSTI]

    Moeller, Karin; Engelke, Hanna; Braeuchle, Christoph; Wagner, Ernst; Bein, Thomas

    2015-01-01

    A new general route for siRNA delivery is presented combining porous core-shell silica nanocarriers with a modularly designed multifunctional block copolymer. Specifically, the internal storage and release of siRNA from mesoporous silica nanoparticles (MSN) with orthogonal core-shell surface chemistry was investigated as a function of pore-size, pore morphology, surface properties and pH. Very high siRNA loading capacities of up to 380 microg/mg MSN were obtained with charge-matched amino-functionalized mesoporous cores, and release profiles show up to 80% siRNA elution after 24 h. We demonstrate that adsorption and desorption of siRNA is mainly driven by electrostatic interactions, which allow for high loading capacities even in medium-sized mesopores with pore diameters down to 4 nm in a stellate pore morphology. The negatively charged MSN shell enabled the association with a block copolymer containing positively charged artificial amino acids and oleic acid blocks, which acts simultaneously as capping func...

  10. Highly Efficient siRNA Delivery from Core-Shell Mesoporous Silica Nanoparticles with Multifunctional Polymer Caps

    E-Print Network [OSTI]

    Karin Moeller; Katharina Mueller; Hanna Engelke; Christoph Braeuchle; Ernst Wagner; Thomas Bein

    2015-09-10

    A new general route for siRNA delivery is presented combining porous core-shell silica nanocarriers with a modularly designed multifunctional block copolymer. Specifically, the internal storage and release of siRNA from mesoporous silica nanoparticles (MSN) with orthogonal core-shell surface chemistry was investigated as a function of pore-size, pore morphology, surface properties and pH. Very high siRNA loading capacities of up to 380 microg/mg MSN were obtained with charge-matched amino-functionalized mesoporous cores, and release profiles show up to 80% siRNA elution after 24 h. We demonstrate that adsorption and desorption of siRNA is mainly driven by electrostatic interactions, which allow for high loading capacities even in medium-sized mesopores with pore diameters down to 4 nm in a stellate pore morphology. The negatively charged MSN shell enabled the association with a block copolymer containing positively charged artificial amino acids and oleic acid blocks, which acts simultaneously as capping function and endosomal release agent. The potential of this multifunctional delivery platform is demonstrated by highly effective cell transfection and siRNA delivery into KB-cells. A luciferase reporter gene knock-down of up to 90% was possible using extremely low cell exposures with only 2.5 microg MSN containing 32 pM siRNA per 100 microL well.

  11. Routine production of copper-64 using 11.7MeV protons

    SciTech Connect (OSTI)

    Jeffery, C. M.; Smith, S. V.; Asad, A. H.; Chan, S.; Price, R. I. [Medical Technology and Physics, Sir Charles Gairdner Hospital, Nedlands, Western Australia, 6009 (Australia); Centre for Forensic Science, University of Western Australia, Nedlands, Western Australia, 6009 (Australia) and ARC Centre of Excellence in A (Australia); ARC Centre of Excellence in Antimatter-Matter Studies, Australian National University, Canberra, ACT 0200 (Australia) and Collider-Accelerator Department, Brookhaven National Laboratory, Upton, NY 11973 (United States); Medical Technology and Physics, Sir Charles Gairdner Hospital, Nedlands, Western Australia, 6009 (Australia); ARC Centre of Excellence in Antimatter-Matter Studies, Australian National University, Canberra, ACT 0200 (Australia) and Imaging and Applied (Australia); Medical Technology and Physics, Sir Charles Gairdner Hospital, Nedlands, Western Australia, 6009 (Australia); Medical Technology and Physics, Sir Charles Gairdner Hospital, Nedlands, Western Australia, 6009 (Australia) and School of Physics, University of Western Australia, Nedlands, Western Australia, 6009 (Australia)

    2012-12-19

    Reliable production of copper-64 ({sup 64}Cu) was achieved by irradiating enriched nickel-64 ({sup 64}Ni, >94.8%) in an IBA 18/9 cyclotron. Nickel-64 (19.1 {+-} 3.0 mg) was electroplated onto an Au disc (125{mu}m Multiplication-Sign 15mm). Targets were irradiated with 11.7 MeV protons for 2 hours at 40{mu}A. Copper isotopes ({sup 60,61,62,64}Cu) were separated from target nickel and cobalt isotopes ({sup 55,57,61}Co) using a single ion exchange column, eluted with varying concentration of low HCl alcohol solutions. The {sup 64}Ni target material was recovered and reused. The {sup 64}Cu production rate was 1.46{+-}0.3MBq/{mu}A.hr/mg{sup 64}Ni(n = 10) (with a maximum of 2.6GBq of {sup 64}Cu isolated after 2hr irradiation at 40uA. Radionuclidic purity of the {sup 64}Cu was 98.7 {+-} 1.6 % at end of separation. Cu content was < 6mg/L (n = 21). The specific activity of {sup 64}Cu was determined by ICP-MS and by titration with Diamsar to be 28.9{+-}13.0GBq/{mu}mol[0.70{+-}0.35Ci/{mu}mol]/({mu}A.hr/mg{sup 64}Ni)(n = 10) and 13.1{+-}12.0GBq/{mu}mol[0.35{+-}0.32Ci/{mu}mol]/({mu}A.hr/mg{sup 64}Ni)(n 9), respectively; which are in agreement, however, further work is required.

  12. Spherical Resorcinol-Formaldehyde Synthesis by Inverse Suspension Polymerization

    SciTech Connect (OSTI)

    Ray, Robert J.; Scrivens, Walter A.; Nash, Charles

    2005-10-21

    Base catalyzed sol-gel polycondensation of resorcinol (1,3-dihydroxybenzene) with formaldehyde by inverse suspension polymerization leads to the formation of uniform, highly cross-linked, translucent, spherical gels, which have increased selectivity and capacity for cesium ion removal from high alkaline solutions. Because of its high selectivity for cesium ion, resorcinol-formaldehyde (R-F) resins are being considered for process scale column radioactive cesium removal by ion-exchange at the Waste Treatment and Immobilization Plant (WTP), which is now under construction at the Hanford site. Other specialty resins such as Superlig{reg_sign} 644 have been ground and sieved and column tested for process scale radioactive cesium removal but show high pressure drops across the resin bed during transition from column regeneration to loading and elution. Furthermore, van Deemter considerations indicate better displacement column chromatography by the use of spherical particle beads rather than irregularly shaped ground or granular particles. In our studies batch contact equilibrium experiments using a high alkaline simulant show a definite increase in cesium loading onto spherical R-F resin. Distribution coefficient (Kd) values ranged from 777 to 429 mL/g in the presence of 0.1M and 0.7M potassium ions, respectively. Though other techniques for making R-F resins have been employed, to our knowledge no one has made spherical R-F resins by inverse suspension polymerization. Moreover, in this study we discuss the data comparisons to known algebraic isotherms used to evaluate ion-exchange resins for WTP plant scale cesium removal operations.

  13. Restenosis of the CYPHER-Select, TAXUS-Express, and Polyzene-F Nanocoated Cobalt-Chromium Stents in the Minipig Coronary Artery Model

    SciTech Connect (OSTI)

    Radeleff, Boris, E-mail: Boris.radeleff@med.uni-heidelberg.de; Thierjung, Heidi; Stampfl, Ulrike; Stampfl, Sibylle; Lopez-Benitez, Ruben; Sommer, Christof [University Heidelberg, Department of Radiology (Germany); Berger, Irina [University Heidelberg, Department of Pathology (Germany); Richter, Goetz M. [University Heidelberg, Department of Radiology (Germany)

    2008-09-15

    PurposeTo date no direct experimental comparison between the CYPHER-Select and TAXUS-Express stents is available. Therefore, we investigated late in-stent stenosis, thrombogenicity, and inflammation, comparing the CYPHER-Select, TAXUS-Express, and custom-made cobalt chromium Polyzene-F nanocoated stents (CCPS) in the minipig coronary artery model.MethodsThe three stent types were implanted in the right coronary artery of 30 minipigs. The primary endpoint was in-stent stenosis assessed by quantitative angiography and microscopy. Secondary endpoints were inflammation and thrombogenicity evaluated by scores for inflammation and immunoreactivity (C-reactive protein and transforming growth factor beta). Follow-up was at 4 and 12 weeks.ResultsStent placement was successful in all animals; no thrombus deposition occurred. Quantitative angiography did not depict statistically significant differences between the three stent types after 4 and 12 weeks. Quantitative microscopy at 4 weeks showed a statistically significant thicker neointima (p = 0.0431) for the CYPHER (105.034 {+-} 62.52 {mu}m) versus the TAXUS (74.864 {+-} 66.03 {mu}m) and versus the CCPS (63.542 {+-} 39.57 {mu}m). At 12 weeks there were no statistically significant differences. Inflammation scores at 4 weeks were significantly lower for the CCPS and CYPHER compared with the TAXUS stent (p = 0.0431). After 12 weeks statistical significance was only found for the CYPHER versus the TAXUS stent (p = 0.0431). The semiquantitative immunoreactivity scores for C-reactive protein and transforming growth factor beta showed no statistically significant differences between the three stent types after 4 and 12 weeks.ConclusionsThe CCPS provided effective control of late in-stent stenosis and thrombogenicity in this porcine model compared with the two drug-eluting stents. Its low inflammation score underscores its noninflammatory potential and might explain its equivalence to the two DES.

  14. New selective anion-exchange resins for nitrate removal from contaminated drinking water and studies on analytical anion-exchange chromatography

    SciTech Connect (OSTI)

    Lockridge, J.E.

    1989-01-01

    Phosphonium resins and ammonium resins of composition resin-R{sub 3}P{sup +}A{sup {minus}} where R is varied from methyl to pentyl were evaluated for nitrate/sulfate selectivity, capacity and nitrate decontamination of drinking water. Phosphonium resins were found to be more nitrate selective and have higher capacities than ammonium resins. A mixed bed process, where nitrate removal and water softening is accomplished in a single column, was also evaluated. A small piece of silver wire, coated with an insoluble silver salt, works well as a selective potentiometric detector for halide ions in ion chromatography. A silver-silver chloride electrode was found to be a selective and reproducible detector for chloride, bromide, iodide, thiocyanate and thiosulfate anions separated by ion chromatography. Calibration curves were non-linear and had slopes ranging from 40 to 60 mV/log concentrations. A working range of 0.05 to 2 mM was used. Two methods for the determination of aluminum by anion chromatography are presented. In the first method, a standard excess of fluoride ion is added to the sample. Evidence is given for the formation of a strong complex of neutral aluminum trifluoride which elutes very quickly from an anion exchange column. The excess fluoride is retained and can be determined. The aluminum concentration can then be related to the difference in fluoride peak height between the sample and standard. In a second method, Al(III) is determined directly by anion chromatography when sodium phthalate is used as an eluent. It was found that Al(III)-phthalate complexes thus formed would show some retention on an anion exchange column. The method is uniquely insensitive to the presence of many foreign cations. Al(III) was successfully determined, by this method, in a 40-fold molar excess of iron(III).

  15. LITERATURE REVIEW FOR OXALATE OXIDATION PROCESSES AND PLUTONIUM OXALATE SOLUBILITY

    SciTech Connect (OSTI)

    Nash, C.

    2012-02-03

    A literature review of oxalate oxidation processes finds that manganese(II)-catalyzed nitric acid oxidation of oxalate in precipitate filtrate is a viable and well-documented process. The process has been operated on the large scale at Savannah River in the past, including oxidation of 20 tons of oxalic acid in F-Canyon. Research data under a variety of conditions show the process to be robust. This process is recommended for oxalate destruction in H-Canyon in the upcoming program to produce feed for the MOX facility. Prevention of plutonium oxalate precipitation in filtrate can be achieved by concentrated nitric acid/ferric nitrate sequestration of oxalate. Organic complexants do not appear practical to sequester plutonium. Testing is proposed to confirm the literature and calculation findings of this review at projected operating conditions for the upcoming campaign. H Canyon plans to commence conversion of plutonium metal to low-fired plutonium oxide in 2012 for eventual use in the Mixed Oxide Fuel (MOX) Facility. The flowsheet includes sequential operations of metal dissolution, ion exchange, elution, oxalate precipitation, filtration, and calcination. All processes beyond dissolution will occur in HB-Line. The filtration step produces an aqueous filtrate that may have as much as 4 M nitric acid and 0.15 M oxalate. The oxalate needs to be removed from the stream to prevent possible downstream precipitation of residual plutonium when the solution is processed in H Canyon. In addition, sending the oxalate to the waste tank farm is undesirable. This report addresses the processing options for destroying the oxalate in existing H Canyon equipment.

  16. Method for selective recovery of PET-usable quantities of [{sup 18}F] fluoride and [{sup 13}N] nitrate/nitrite from a single irradiation of low-enriched [{sup 18}O] water

    DOE Patents [OSTI]

    Ferrieri, R.A.; Schlyer, D.J.; Shea, C.

    1995-06-13

    A process for simultaneously producing PET-usable quantities of [{sup 13}N]NH{sub 3} and [{sup 18}F]F{sup {minus}} for radiotracer synthesis is disclosed. The process includes producing [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}}and [{sup 18}F]F{sup {minus}} simultaneously by exposing a low-enriched (20%-30%) [{sup 18}O]H{sub 2}O target to proton irradiation, sequentially isolating the [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}} and [{sup 18}F]F{sup {minus}} from the [{sup 18}O]H{sub 2}O target, and reducing the [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}} to [{sup 13}N]NH{sub 3}. The [{sup 13}N]NH{sub 3} and [{sup 18}F]F{sup {minus}} products are then conveyed to a laboratory for radiotracer applications. The process employs an anion exchange resin for isolation of the isotopes from the [{sup 18}O]H{sub 2}O, and sequential elution of [{sup 13}N]NO{sub 2}{sup {minus}}/NO{sub 3}{sup {minus}} and [{sup 18}F]F{sup {minus}} fractions. Also the apparatus is disclosed for simultaneously producing PET-usable quantities of [{sup 13}N]NH{sub 3} and [{sup 18}F]F{sup {minus}} from a single irradiation of a single low-enriched [{sup 18}O]H{sub 2}O target. 2 figs.

  17. RAPID METHOD FOR DETERMINATION OF {sup 228}Ra IN WATER SAMPLES

    SciTech Connect (OSTI)

    Maxwell, S.

    2012-09-05

    A new rapid method for the determination of {sup 228}Ra in natural water samples has been developed at the SRNL/EBL (Savannah River National Lab/ Environmental Bioassay Laboratory) that can be used for emergency response or routine samples. While gamma spectrometry can be employed with sufficient detection limits to determine {sup 228}Ra in solid samples (via {sup 228}Ac) , radiochemical methods that employ gas flow proportional counting techniques typically provide lower MDA (Minimal Detectable Activity) levels for the determination of {sup 228}Ra in water samples. Most radiochemical methods for {sup 228}Ra collect and purify {sup 228}Ra and allow for {sup 228}Ac daughter ingrowth for ~36 hours. In this new SRNL/EBL approach, {sup 228}Ac is collected and purified from the water sample without waiting to eliminate this delay. The sample preparation requires only about 4 hours so that {sup 228}Ra assay results on water samples can be achieved in < 6 hours. The method uses a rapid calcium carbonate precipitation enhanced with a small amount of phosphate added to enhance chemical yields (typically >90%), followed by rapid cation exchange removal of calcium. Lead, bismuth, uranium, thorium and protactinium isotopes are also removed by the cation exchange separation. {sup 228}Ac is eluted from the cation resin directly onto a DGA Resin cartridge attached to the bottom of the cation column to purify {sup 228}Ac. DGA Resin also removes lead and bismuth isotopes, along with Sr isotopes and {sup 90}Y. La is used to determine {sup 228}Ac chemical yield via ICP-MS, but {sup 133}Ba can also be used instead if ICP-MS assay is not available. Unlike some older methods, no lead or strontium holdback carriers or continual readjustment of sample pH is required.

  18. 230Th-234U Age-Dating Uranium by Mass Spectrometry

    SciTech Connect (OSTI)

    Williams, R W; Gaffney, A M

    2012-04-18

    This is the standard operating procedure used by the Isotope Ratio Mass Spectrometry Group of the Chemical Sciences Division at LLNL for the preparation of a sample of uranium oxide or uranium metal for {sup 230}Th-{sup 234}U age-dating. The method described here includes the dissolution of a sample of uranium oxide or uranium metal, preparation of a secondary dilution, spiking of separate aliquots for uranium and thorium isotope dilution measurements, and purification of uranium and thorium aliquots for mass spectrometry. This SOP may be applied to uranium samples of unknown purity as in a nuclear forensic investigation, and also to well-characterized samples such as, for example, U{sub 3}O{sub 8} and U-metal certified reference materials. The sample of uranium is transferred to a quartz or PFA vial, concentrated nitric acid is added and the sample is heated on a hotplate at approximately 100 C for several hours until it dissolves. The sample solution is diluted with water to make the solution approximately 4 M HNO{sub 3} and hydrofluoric acid is added to make it 0.05 M HF. A secondary dilution of the primary uranium solution is prepared. Separate aliquots for uranium and thorium isotope dilution measurements are taken and spiked with {sup 233}U and {sup 229}Th, respectively. The spiked aliquot for uranium isotope dilution analysis is purified using EiChrom UTEVA resin. The spiked aliquot for thorium isotope dilution analysis is purified by, first, a 1.8 mL AG1x8 resin bed in 9 M HCl on which U adsorbs and Th passes through; second, adsorbing Th on a 1 mL AG1x8 resin bed in 8 M HNO{sub 3} and then eluting it with 9 M HCl followed by 0.1 M HCl + 0.005 M HF; and third, by passing the Th through a final 1.0 mL AG1x8 resin bed in 9 M HCl. The mass spectrometry is performed using the procedure 'Th and U Mass Spectrometry for {sup 230}Th-{sup 234}U Age Dating'.

  19. Development Of Ion Chromatography Methods To Support Testing Of The Glycolic Acid Reductant Flowsheet In The Defense Waste Processing Facility

    SciTech Connect (OSTI)

    Wiedenman, B. J.; White, T. L.; Mahannah, R. N.; Best, D. R.; Stone, M. E.; Click, D. R.; Lambert, D. P.; Coleman, C. J.

    2013-10-01

    Ion Chromatography (IC) is the principal analytical method used to support studies of Sludge Reciept and Adjustment Tank (SRAT) chemistry at DWPF. A series of prior analytical ''Round Robin'' (RR) studies included both supernate and sludge samples from SRAT simulant, previously reported as memos, are tabulated in this report.2,3 From these studies it was determined to standardize IC column size to 4 mm diameter, eliminating the capillary column from use. As a follow on test, the DWPF laboratory, the PSAL laboratory, and the AD laboratory participated in the current analytical RR to determine a suite of anions in SRAT simulant by IC, results also are tabulated in this report. The particular goal was to confirm the laboratories ability to measure and quantitate glycolate ion. The target was + or - 20% inter-lab agreement of the analyte averages for the RR. Each of the three laboratories analyzed a batch of 12 samples. For each laboratory, the percent relative standard deviation (%RSD) of the averages on nitrate, glycolate, and oxalate, was 10% or less. The three laboratories all met the goal of 20% relative agreement for nitrate and glycolate. For oxalate, the PSAL laboratory reported an average value that was 20% higher than the average values reported by the DWPF laboratory and the AD laboratory. Because of this wider window of agreement, it was concluded to continue the practice of an additional acid digestion for total oxalate measurement. It should also be noted that large amounts of glycolate in the SRAT samples will have an impact on detection limits of near eluting peaks, namely Fluoride and Formate. A suite of scoping experiments are presented in the report to identify and isolate other potential interlaboratory disceprancies. Specific ion chromatography inter-laboratory method conditions and differences are tabulated. Most differences were minor but there are some temperature control equipment differences that are significant leading to a recommendation of a heated jacket for analytical columns that are remoted for use in radiohoods. A suggested method improvement would be to implement column temperture control at a temperature slightly above ambient to avoid peak shifting due to temperature fluctuations. Temperature control in this manner would improve short and longer term peak retention time stability. An unknown peak was observed during the analysis of glycolic acid and SRAT simulant. The unknown peak was determined to best match diglycolic acid. The development of a method for acetate is summaraized, and no significant amount of acetate was observed in the SRAT products tested. In addition, an alternative Gas Chromatograph (GC) method for glycolate is summarized.

  20. Groundwork for Universal Canister System Development

    SciTech Connect (OSTI)

    Price, Laura L.; Gross, Mike; Prouty, Jeralyn L.; Rigali, Mark J.; Craig, Brian; Han, Zenghu; Lee, John Hok; Liu, Yung; Pope, Ron; Connolly, Kevin; Feldman, Matt; Jarrell, Josh; Radulescu, Georgeta; Scaglione, John; Wells, Alan

    2015-09-01

    The mission of the United States Department of Energy's Office of Environmental Management is to complete the safe cleanup of the environmental legacy brought about from five decades of nuclear weapons development and go vernment - sponsored nuclear energy re search. S ome of the waste s that that must be managed have be en identified as good candidates for disposal in a deep borehole in crystalline rock (SNL 2014 a). In particular, wastes that can be disposed of in a small package are good candidates for this disposal concept. A canister - based system that can be used for handling these wastes during the disposition process (i.e., storage, transfers, transportation, and disposal) could facilitate the eventual disposal of these wastes. This report provides information for a program plan for developing specifications regarding a canister - based system that facilitates small waste form packaging and disposal and that is integrated with the overall efforts of the DOE's Office of Nuclear Energy Used Fuel Dis position Camp aign's Deep Borehole Field Test . Groundwork for Universal Ca nister System Development September 2015 ii W astes to be considered as candidates for the universal canister system include capsules containing cesium and strontium currently stored in pools at the Hanford Site, cesium to be processed using elutable or nonelutable resins at the Hanford Site, and calcine waste from Idaho National Laboratory. The initial emphasis will be on disposal of the cesium and strontium capsules in a deep borehole that has been drilled into crystalline rock. Specifications for a universal canister system are derived from operational, performance, and regulatory requirements for storage, transfers, transportation, and disposal of radioactive waste. Agreements between the Department of Energy and the States of Washington and Idaho, as well as the Deep Borehole Field Test plan provide schedule requirements for development of the universal canister system . Future work includes collaboration with the Hanford Site to move the cesium and strontium capsules into dry storage, collaboration with the Deep Borehole Field Tes t to develop surface handling and emplacement techniques and to develop the waste package design requirements, developing universal canister system design options and concepts of operations, and developing system analysis tools. Areas in which f urther research and development are needed include material properties and structural integrity, in - package sorbents and fillers, waste form tolerance to heat and postweld stress relief, waste package impact limiters, sensors, cesium mobility under downhol e conditions, and the impact of high pressure and high temperature environment on seals design.

  1. LOW ACTIVITY WASTE FEED SOLIDS CARACTERIZATION AND FILTERABILITY TESTS

    SciTech Connect (OSTI)

    McCabe, D.; Crawford, C.; Duignan, M.; Williams, M.; Burket, P.

    2014-04-03

    The primary treatment of the tank waste at the DOE Hanford site will be done in the Waste Treatment and Immobilization Plant (WTP) that is currently under construction. The baseline plan for the WTP Pretreatment facility is to treat the waste, splitting it into High Level Waste (HLW) feed and Low Activity Waste (LAW) feed. Both waste streams are then separately vitrified as glass and sealed in canisters. The LAW glass will be disposed onsite in the Integrated Disposal Facility (IDF). There are currently no plans to treat the waste to remove technetium in the WTP Pretreatment facility, so its disposition path is the LAW glass. Options are being explored to immobilize the LAW portion of the tank waste, i.e., the LAW feed from the WTP Pretreatment facility. Removal of {sup 99}Tc from the LAW Feed, followed by off-site disposal of the {sup 99}Tc, would eliminate a key risk contributor for the IDF Performance Assessment (PA) for supplemental waste forms, and has potential to reduce treatment and disposal costs. Washington River Protection Solutions (WRPS) is developing some conceptual flow sheets for LAW treatment and disposal that could benefit from technetium removal. One of these flowsheets will specifically examine removing {sup 99}Tc from the LAW feed stream to supplemental immobilization. The conceptual flow sheet of the {sup 99}Tc removal process includes a filter to remove insoluble solids prior to processing the stream in an ion exchange column, but the characteristics and behavior of the liquid and solid phases has not previously been investigated. This report contains results of testing of a simulant that represents the projected composition of the feed to the Supplemental LAW process. This feed composition is not identical to the aqueous tank waste fed to the Waste Treatment Plant because it has been processed through WTP Pretreatment facility and therefore contains internal changes and recycle streams that will be generated within the WTP process. Although a Supplemental LAW feed simulant has previously been prepared, this feed composition differs from that simulant because those tests examined only the fully soluble aqueous solution at room temperature, not the composition formed after evaporation, including the insoluble solids that precipitate after it cools. The conceptual flow sheet for Supplemental LAW immobilization has an option for removal of {sup 99}Tc from the feed stream, if needed. Elutable ion exchange has been selected for that process. If implemented, the stream would need filtration to remove the insoluble solids prior to processing in an ion exchange column. The characteristics, chemical speciation, physical properties, and filterability of the solids are important to judge the feasibility of the concept, and to estimate the size and cost of a facility. The insoluble solids formed during these tests were primarily natrophosphate, natroxalate, and a sodium aluminosilicate compound. At the elevated temperature and 8 M [Na+], appreciable insoluble solids (1.39 wt%) were present. Cooling to room temperature and dilution of the slurry from 8 M to 5 M [Na+] resulted in a slurry containing 0.8 wt% insoluble solids. The solids (natrophosphate, natroxalate, sodium aluminum silicate, and a hydrated sodium phosphate) were relatively stable and settled quickly. Filtration rates were in the range of those observed with iron-based simulated Hanford tank sludge simulants, e.g., 6 M [Na+] Hanford tank 241-AN-102, even though their chemical speciation is considerably different. Chemical cleaning of the crossflow filter was readily accomplished with acid. As this simulant formulation was based on an average composition of a wide range of feeds using an integrated computer model, this exact composition may never be observed. But the test conditions were selected to enable comparison to the model to enable improving its chemical prediction capability.

  2. Elemental Fluorine-18 Gas: Enhanced Production and Availability

    SciTech Connect (OSTI)

    VanBrocklin, Henry F. [Department of Radiology and Biomedical Imaging

    2011-12-01

    The overall objective of this project was to develop an efficient, reproducible and reliable process for the preparation of fluorine-18 labeled fluorine gas ([¹?F]F?) from readily available cyclotron-produced [¹?F]fluoride ion. The two step process entailed the production of [¹?F]fluoromethane with subsequent conversion to [¹?F]F? by electric discharge of [¹?F]fluoromethane in the presence of carrier nonradioactive F? gas. The specific goals of this project were i) to optimize the preparation of [¹?F]fluoromethane from [¹?F]fluoride ion; ii) to develop a prototype automated system for the production of [¹?F]F? from [¹?F]fluoride ion and iii) develop a compact user friendly automated system for the preparation of [¹?F]F? with initial synthesis of fluorine-18 labeled radiotracers. Over the last decade there has been an increased interest in the production of "non-standard" positron-emitting isotopes for the preparation of new radiotracers for a variety of applications including medical imaging and therapy. The increased availability of these isotopes from small biomedical cyclotrons has prompted their use in labeling radiotracers. In much the same way the production of [¹?F]F? gas has been known for several decades. However, access to [¹?F]F? gas has been limited to those laboratories with the means (e.g. F? targetry for the cyclotron) and the project-based need to work with [¹?F]F? gas. Relatively few laboratories, compared to those that produce [¹?F]fluoride ion on a daily basis, possess the capability to produce and use [¹?F]F? gas. A simplified, reliable system employing [¹?F]fluoride ion from cyclotron targetry systems that are already in place coupled with on-demand production of the [¹?F]F? gas would greatly enhance its availability. This would improve the availability of [¹?F]F? gas and promote further work with a valuable precursor. The major goals of the project were accomplished over the funding period. The preparation of ¹?F]fluoromethane has been automated with reproducible yields greater than 90% conversion from [¹?F]fluoride ion. A trap and release system was established for the [¹?F]fluoride ion concentration and direct elution of the [¹?F]fluoride ion into the reaction vial with the appropriate base and precursor in DMSO. Other solvents were also investigated. The production time for [¹?F]fluoromethane is less than 10 minutes. An automated system for the [¹?F]F? gas production from the [18F]fluoromethane has been developed. The unit coupled to the [¹?F]fluoromethane system permits the on demand production of [¹?F]F? gas. In less than 30 minutes, mCi quantities of [¹?F]F? gas were produced. Several variables for the [¹?F]F? gas production were investigated and a set of parameters for reproducible operation were determined. These parameters included discharge chamber size, carrier gas (He, Ne, Ar), discharge time, discharge current, mass of F? gas added to the chamber. FDOPA and EF5 were used to test the reactivity of the [¹?F]F? gas. Both products were produced in low to modest yield. The ready availability of [¹?F]F? gas has potential impact to advance both DOE mission-driven initiatives and nuclear medicine initiatives through other federally funded agencies such as NIH and DoD. New reactions involving the use of [¹?F]F? gas will lead to direct labeling of new radiotracers and intermediates as well as new fluorine-18 labeled synthons that may be further reacted with other reagents to provide useful fluorine-18 labeled compounds. New tracers to understand and follow plant and microbial metabolism as well as new tracers for nuclear medicine applications, that have been either difficult to obtain or never produced due to the limited availability of [¹?F]F? gas, may be prepared using the techniques developed .

  3. Ruthenium Behavior at Phase Separation of Borosilicate Glass-12259

    SciTech Connect (OSTI)

    Enokida, Youichi [Graduate School of Engineering, Nagoya University, Nagoya, 463-8603 (Japan); Sawada, Kayo [EcoTopia Science Institute, Nagoya University, Nagoya, 463-8603 (Japan)

    2012-07-01

    The Rokkasho reprocessing plant (RRP) located in Aomori, Japan, vitrifies high level waste (HLW) into a borosilicate glass. The HLW is generated from the reprocessing of spent fuel and contains ruthenium (Ru) and other platinum group metals (PGMs). Based on the recent consequences after a huge earthquake that occurred in Japan, a hypothetical blackout was postulated for the RRP to address additional safety analysis requirements. During a prolonged blackout, the borosilicate glass could phase separate due to cooling of the glass in the melter. The Ru present in the glass matrix could migrate into separate phases and impact the durability of the borosilicate glass. The durability of the glass is important for quality assurance and performance assessment of the vitrified HLW. A fundamental study was performed at an independent university to understand the impact of a prolonged blackout. Simulated HLW glasses were prepared for the RRP, and the Ru behavior in phase separated glasses was studied. The simulated HLW glasses contained nonradioactive elements and PGMs. The glass compositions were then altered to enhance the formation of the phase-separated glasses when subjected to thermal treatment at 700 deg. C for 24 hours. The synthesized simulated glasses contained 1.1 % Ru by weight as ruthenium dioxide (RuO{sub 2}). A portion of the RuO{sub 2} formed needle-shaped crystals in the glass specimens. After the thermal treatment, the glass specimen had separated into two phases. One of the two phases was a B{sub 2}O{sub 3} rich phase, and the other phase was a SiO{sub 2} rich phase. The majority of the chemical species in the B{sub 2}O{sub 3} rich phase was leached away with the Material Characterization Center-3 (MCC-3) protocol standardized by the Pacific Northwest National Laboratory using an aqueous low-concentrated nitric acid solution, but the leaching of the Ru fraction was very limited; less than 1% of the original Ru content. The Ru leaching was much less than those of the other elements, and the needle-shaped crystals of RuO{sub 2} were observed in the B{sub 2}O{sub 3} rich phase in the specimen after the leaching test. Another experiment was performed using another glass specimen which had been prepared with the same frits, but used reagent RuO{sub 2} of granular shape at lower content (0.0073% by weight as RuO{sub 2}). The leached fractions of elements for the latter specimen increased to almost the same fraction (more than 10% of the original Ru content) as observed for boron and sodium, when the phase separated glass was leached using the MMC-3 protocol with non-acidic de-ionized water. Based on the results of this study, it was concluded that needle-shaped RuO{sub 2} crystals are contained in the B{sub 2}O{sub 3}-rich phase after phase separation of the borosilicate glass after a hypothetical blackout. The leaching fraction for the needle-shaped RuO{sub 2} present in the phase separated glass is much lower than those for boron or sodium. Ruthenium behavior has been studied for a hypothetical loss of cooling in the liquid fed ceramic melter for high level waste by taking into account the phase separation of borosilicate glass. The needle-shaped crystal of ruthenium dioxide after bi-nodal-type phase separation of the borosilicate glass at 700 deg. C migrated into the B{sub 2}O{sub 3} rich phase, but remained without dissolution by an acidic aqueous solution. Additionally, granular ruthenium dioxide can be a morphological form of ruthenium after bimodal-type phase separation of the vitrified high level waste with borosilicate glass media. After the phase separation of the borosilicate glass, the crystal shape of the ruthenium dioxide is either needle-shaped or granular, and the leachable fraction of ruthenium is relatively much lower than those of major components (boron and sodium) in the vitrified borosilicate glass. The fraction of leached ruthenium increased to almost the same fraction as observed for boron and sodium when the phase-separated glass was leached with ultrapure water. (authors)