National Library of Energy BETA

Sample records for nf nf nf

  1. TNAP, a Novel Repressor of NF-B-inducing Kinase, Suppresses NF-B Activation*

    E-Print Network [OSTI]

    Hu, Wen-Hui

    responses, oncogenesis, apoptosis, and neuronal signaling (1­5). To date two principal pathways for NF- B

  2. Correction to NF 3 , the greenhouse gas missing from Kyoto

    E-Print Network [OSTI]

    Prather, Michael J; Hsu, Juno

    2010-01-01

    J. Hsu (2008), NF 3 , the greenhouse gas missing from Kyoto,Correction to “NF 3 , the greenhouse gas missing from Kyoto”2010), Correction to “NF 3 , the greenhouse gas missing from

  3. NF kappa B expression and matrix metalloproteinase activity in hypertension

    E-Print Network [OSTI]

    Wu, Kwan-I

    2009-01-01

    without PDTC treatment by gelatin gel zymography. Chapter 2Labeling of NF-?B………………………………14 Gelatin Gel ZymographyPDTC treatment measured by gelatin zymography…………………34

  4. Erratum: NF3, the greenhouse gas missing from Kyoto (Geophysical Research Letters (2010) 37 (L11807))

    E-Print Network [OSTI]

    Prather, MJ; Hsu, J

    2010-01-01

    J. Hsu (2008), NF 3 , the greenhouse gas missing from Kyoto,Correction to “NF 3 , the greenhouse gas missing from Kyoto”2010), Correction to “NF 3 , the greenhouse gas missing from

  5. Cloning and expression of equine NF-kB2 

    E-Print Network [OSTI]

    Mirhosseini, Negin

    2009-05-15

    ........................ 51 xi LIST OF TABLES Page Table 1 Primers to generate full length NF-?B2... of the interaction between EIAV S2 and equine NF-?B in the yeast two hybrid system........................... 43 Table 7 Primers and probes for quantitative PCR.......................................... 48 1 CHAPTER I INTRODUCTION 1 Equine Infectious...

  6. Effects of water chemistry on NF/RO membrane structure and performance

    E-Print Network [OSTI]

    Mo, Yibing

    2013-01-01

    potable water and seawater desalination. NF/RO are effectivemembranes under seawater conditions, Desalination, 247 (

  7. NF 3 , the greenhouse gas missing from Kyoto

    E-Print Network [OSTI]

    Prather, Michael J; Hsu, Juno

    2008-01-01

    of high-GWP, long-lived, greenhouse gases like NF 3 shouldbe included in the national greenhouse gas inventories onceto expand the list of greenhouse gases for the second

  8. NF-?B Fans the Flames of Lung Carcinogenesis

    E-Print Network [OSTI]

    Jacks, Tyler E.

    This perspective on Deng et al. (beginning on p. 424 in this issue of the journal) examines the link between NF-?B and lung tumorigenesis. Experiments in genetically engineered mouse models of lung cancers are elucidating ...

  9. Haploid reporter screens aimed at identification of NF-kappaB regulators

    E-Print Network [OSTI]

    Lee, Clarissa Ci

    2014-01-01

    Nuclear factor-kappaB (NF-KB) is a family of transcription factors that are essential for execution of both the innate and the adaptive immune response. NF-KB regulates hundreds of genes involved in critical processes such ...

  10. Finite density phase transition of QCD with $N_f=4$ and $N_f=2$ using canonical ensemble method

    E-Print Network [OSTI]

    Anyi Li; Andrei Alexandru; Keh-Fei Liu; Xiangfei Meng

    2010-10-01

    In a progress toward searching for the QCD critical point, we study the finite density phase transition of $N_f = 4$ and 2 lattice QCD at finite temperature with the canonical ensemble approach. We develop a winding number expansion method to accurately project out the particle number from the fermion determinant which greatly extends the applicable range of baryon number sectors to make the study feasible. Our lattice simulation was carried out with the clover fermions and improved gauge action. For a given temperature, we calculate the baryon chemical potential from the canonical approach to look for the mixed phase as a signal for the first order phase transition. In the case of $N_f=4$, we observe an "S-shape" structure in the chemical potential-density plane due to the surface tension of the mixed phase in a finite volume which is a signal for the first order phase transition. We use the Maxwell construction to determine the phase boundaries for three temperatures below $T_c$. The intersecting point of the two extrapolated boundaries turns out to be at the expected first order transition point at $T_c$ with $\\mu = 0$. This serves as a check for our method of identifying the critical point. We also studied the $N_f =2$ case, but do not see a signal of the mixed phase for temperature as low as 0.83 $T_c$.

  11. Response and Resistance to NF-?B Inhibitors in Mouse Models of Lung Adenocarcinoma

    E-Print Network [OSTI]

    Xue, Wen

    Lung adenocarcinoma is a leading cause of cancer death worldwide. We recently showed that genetic inhibition of the NF-?B pathway affects both the initiation and the maintenance of lung cancer, identifying this pathway as ...

  12. Requirement for NF-?B signalling in a mouse model of lung adenocarcinoma

    E-Print Network [OSTI]

    Meylan, Etienne

    NF-?B transcription factors function as crucial regulators of inflammatory and immune responses as well as of cell survival. They have also been implicated in cellular transformation and tumorigenesis. However, despite ...

  13. Interleukin-6 upregulates paraoxonase 1 gene expression via an AKT/NF-?B-dependent pathway

    SciTech Connect (OSTI)

    Cheng, Chi-Chih [Department of Research, Taichung Veterans General Hospital, Taichung, Taiwan (China)] [Department of Research, Taichung Veterans General Hospital, Taichung, Taiwan (China); Hsueh, Chi-Mei [Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan (China)] [Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan (China); Chen, Chiu-Yuan [Graduate Institute of Natural Healing Sciences, Nanhua University, Chiayi, Taiwan (China)] [Graduate Institute of Natural Healing Sciences, Nanhua University, Chiayi, Taiwan (China); Chen, Tzu-Hsiu, E-mail: hsiu@mail.chna.edu.tw [Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan (China)] [Department of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan, Taiwan (China); Hsu, Shih-Lan, E-mail: h2326@vghtc.gov.tw [Department of Research, Taichung Veterans General Hospital, Taichung, Taiwan (China) [Department of Research, Taichung Veterans General Hospital, Taichung, Taiwan (China); Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, Taiwan (China)

    2013-07-19

    Highlights: •IL-6 could induce PON1 gene expression. •IL-6 increased NF-?B protein expression and NF-?B-p50 and -p65 subunits nuclear translocation. •IL-6-induced PON1 up-regulation was through an AKT/NF-?B pathway. -- Abstract: The aim of this study is to investigate the relationship between paraoxonase 1 (PON1) and atherosclerosis-related inflammation. In this study, human hepatoma HepG2 cell line was used as a hepatocyte model to examine the effects of the pro-inflammatory cytokines on PON1 expression. The results showed that IL-6, but not TNF-? and IL-1?, significantly increased both the function and protein level of PON1; data from real-time RT-PCR analysis revealed that the IL-6-induced PON1 expression occurred at the transcriptional level. Increase of I?B kinase activity and I?B phosphorylation, and reduction of I?B protein level were also observed in IL-6-treated HepG2 cells compared with untreated culture. This event was accompanied by increase of NF-?B-p50 and -p65 nuclear translocation. Moreover, treatment with IL-6 augmented the DNA binding activity of NF-?B. Furthermore, pharmacological inhibition of NF-?B activation by PDTC and BAY 11-7082, markedly suppressed the IL-6-mediated PON1 expression. In addition, IL-6 increased the levels of phosphorylated protein kinase B (PKB, AKT). An AKT inhibitor LY294002 effectively suppressed IKK/I?B/NF-?B signaling and PON1 gene expression induced by IL-6. Our findings demonstrate that IL-6 upregulates PON1 gene expression through an AKT/NF-?B signaling axis in human hepatocyte-derived HepG2 cell line.

  14. A test of first order scaling in Nf=2 QCD

    E-Print Network [OSTI]

    G. Cossu; M. D'Elia; A. Di Giacomo; C. Pica

    2007-09-30

    We complete our analysis of Nf=2 QCD based on the lattice staggered fermion formulation. Using a series of Monte Carlo simulations at fixed (amq*Ls^yh) one is able to test the universality class with given critical exponent yh. This strategy has been used to test the O(4) universality class and it has been presented at the previous Lattice conferences. No agreement was found with simulations in the mass range amq=[0.01335,0.15] using lattices with Ls=16 up to 32 and Lt=4. With the same strategy, we now investigate the possibility of a first order transition using a new set of Monte Carlo data corresponding to yh=3 in the same mass and volume range as the one used for O(4). A substantial agreement is observed both in the specific heat scaling and in the scaling of the chiral condensate, while the chiral susceptibilities still presents visible deviation from scaling in the mass range explored.

  15. Development of NF3 Deposit Removal Technology for the Portsmouth Gaseous Diffusion Plant

    SciTech Connect (OSTI)

    Scheele, R.D.; McNamara, B.K.; Rapko, B.M.; Edwards, M.K.; Kozelisky, A.E.; Daniel, R.C. [Battelle Pacific Northwest Division, PO Box 999, Battelle Blvd, Richland, Washington 99352 (United States); McSweeney, T.I.; Maharas, S.J.; Weaver, P.J.; Iwamasa, K.J. [Battelle Columbus Operations, 505 King Avenue, Columbus, Ohio 43201 (United States); Kefgen, R.B. [WASTREN, Inc., 1864 Shyville Road, Piketon, Ohio 45661 (United States)

    2006-07-01

    This paper summarizes the Battelle, Stoller, and WASTREN (BSW) team's efforts, to date, in support of the United States Department of Energy's plans to remove uranium and technetium deposits before decommissioning the Portsmouth Gaseous Diffusion Plant. The BSW team investigated nitrogen trifluoride (NF{sub 3}) as a safer yet effective alternative gaseous treatment to the chlorine trifluoride (ClF{sub 3})-elemental fluorine (F{sub 2}) treatment currently used to remove uranium and technetium deposits from the uranium enrichment cascade. Both ClF{sub 3} and F{sub 2} are highly reactive, toxic, and hazardous gases, while NF{sub 3}, although toxic [1], is no more harmful than moth balls [2]. BSW's laboratory thermo-analytical and laboratory-scale prototype studies with NF{sub 3} established that thermal NF{sub 3} can effectively remove likely and potential uranium (UO{sub 2}F{sub 2} and UF{sub 4}) and technetium deposits (a surrogate deposit material, TcO{sub 2}, and pertechnetates) by conversion to volatile compounds. Our engineering evaluations suggest that NF{sub 3}'s effectiveness could be enhanced by combining with a lesser concentration of ClF{sub 3}. BSW's and other's studies indicate compatibility with Portsmouth materials of construction (aluminum, copper, and nickel). (authors)

  16. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  17. CARD6 Is a Modulator of NF-B Activation by Nod1-and Cardiak-mediated Pathways*

    E-Print Network [OSTI]

    CARD6 Is a Modulator of NF- B Activation by Nod1- and Cardiak-mediated Pathways* Received specific binding of this CARD6-encoding protein to Nod1 (CARD4), Cardiak (Rip2/Rick), NAC (NALP1/DEFCAP implicated in NF- B and caspase-1 activation but not to other CARD family proteins. Cardiak and Nod1 (but

  18. NF-?B1 Inhibits TLR-Induced IFN-? Production in Macrophages Through TPL-2-dependent ERK Activation

    E-Print Network [OSTI]

    Yang, Huei-Ting

    Although NF-?B1 p50/p105 has critical roles in immunity, the mechanism by which NF-?B1 regulates inflammatory responses is unclear. In this study, we analyzed the gene expression profile of LPS-stimulated Nfkb1?/? macrophages ...

  19. Investigating the role of intra- and extra-cellular modulators of the transcription factor NF-kappaB

    E-Print Network [OSTI]

    Nguyen, Hung Nhat

    2011-01-01

    The nuclear factor-kappa B (NF-kappaB) family of transcription factors play a pivotal role in the inflammatory and immune responses, as well as cell growth and survival. Since its discovery 25 years ago, NF-kappaB has ...

  20. 1 Managed by UT-Battelle for the U.S. Department of Energy NF Splash Mitigation 12 July 2011

    E-Print Network [OSTI]

    McDonald, Kirk

    1 Managed by UT-Battelle for the U.S. Department of Energy NF Splash Mitigation 12 July 2011 for comparison & final determination #12;3 Managed by UT-Battelle for the U.S. Department of Energy NF Splash Neutrino Factory Mercury Pool Splash Mitigation V.B. Graves IDS Videoconference July 12, 2011 #12;2 Managed

  1. VALIDATION OF EAF-2010 (N,G) AND (N,F) REACTIONS 1. INTEGRAL ACTIVATION DATA BASE FOR (N,G) AND (N,F) REACTIONS

    E-Print Network [OSTI]

    VALIDATION OF EAF-2010 (N,G) AND (N,F) REACTIONS 1. INTEGRAL ACTIVATION DATA BASE FOR (N,G) AND (N IRRAGIAMENTO SIC A FNG, supplied by M Pillon used at FNG for integral exps [175 group Vitamin-E weighting Pillon used at FNG for integral exps [175 group Vitamin-E weighting]. UKAEA-FUS-547 7 fng_tung.asc 175

  2. Instruction sheet for the consolidation of the standard NF EN 693 dated September 2001 and its amendment A1 Machine tools : Safety : Hydraulic presses

    E-Print Network [OSTI]

    Association Française de Normalisation. Paris

    2009-01-01

    Instruction sheet for the consolidation of the standard NF EN 693 dated September 2001 and its amendment A1

  3. Microstructural Evolution in Two Variants of NF709 at 1023 and 1073 K

    E-Print Network [OSTI]

    Cambridge, University of

    and that could invalidate the extrapolations of short-term creep test data, and to identify any precipitates.K.D.H. BHADESHIA The microstructural state of two grades of the creep-resistant austenitic stainless steels NF709 regarded as one of the best austenitic steels for elevated temperature applications because of its creep

  4. Purinergic signaling is required for fluid shear stress-induced NF-{kappa}B translocation in osteoblasts

    SciTech Connect (OSTI)

    Genetos, Damian C., E-mail: dgenetos@ucdavis.edu [Department of Anatomy, Cell Biology, and Physiology, School of Veterinary Medicine, University of California, Davis, CA (United States); Karin, Norman J. [Cell Biology and Biochemistry, Pacific Northwest National Laboratory, Richland, WA (United States)] [Cell Biology and Biochemistry, Pacific Northwest National Laboratory, Richland, WA (United States); Geist, Derik J. [Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN (United States)] [Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN (United States); Donahue, Henry J. [Division of Musculoskeletal Sciences, Department of Orthopaedics and Rehabilitation, Pennsylvania State College of Medicine, Hershey, PA (United States)] [Division of Musculoskeletal Sciences, Department of Orthopaedics and Rehabilitation, Pennsylvania State College of Medicine, Hershey, PA (United States); Duncan, Randall L. [Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN (United States)] [Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN (United States)

    2011-04-01

    Fluid shear stress regulates gene expression in osteoblasts, in part by activation of the transcription factor NF-{kappa}B. We examined whether this process was under the control of purinoceptor activation. MC3T3-E1 osteoblasts under static conditions expressed the NF-{kappa}B inhibitory protein I{kappa}B{alpha} and exhibited cytosolic localization of NF-{kappa}B. Under fluid shear stress, I{kappa}B{alpha} levels decreased, and concomitant nuclear localization of NF-{kappa}B was observed. Cells exposed to fluid shear stress in ATP-depleted medium exhibited no significant reduction in I{kappa}B{alpha}, and NF-{kappa}B remained within the cytosol. Similar results were found using oxidized ATP or Brilliant Blue G, P2X{sub 7} receptor antagonists, indicating that the P2X{sub 7} receptor is responsible for fluid shear-stress-induced I{kappa}B{alpha} degradation and nuclear accumulation of NF-{kappa}B. Pharmacologic blockage of the P2Y6 receptor also prevented shear-induced I{kappa}B{alpha} degradation. These phenomena involved neither ERK1/2 signaling nor autocrine activation by P2X{sub 7}-generated lysophosphatidic acid. Our results suggest that fluid shear stress regulates NF-{kappa}B activity through the P2Y{sub 6} and P2X{sub 7} receptor.

  5. Coal dust contiguity-induced changes in the concentration of TNF- and NF- B p65 on the ocular surface

    SciTech Connect (OSTI)

    Sun, Z.Y.; Hong, J.; Liu, Z.Y.; Jin, X.D.; Gu, C.H.

    2009-07-01

    To observe the influence of coal dust on ocular surface of coal miners and rabbits with coal dust contiguity on expression TNF- and NF- Bp65 and dry eye occurrence. Expression TNF- and NF- Bp65 in ocular surface were determined. Results showed tear production, BUT and lysozyme decreased for coal miners and rabbits with coal dust contiguity. Coal dust exposure was linked to development of xerophthalmia, and induced a higher expression of NF- B p65 and TNF- perhaps as a mechanism to resist coal dust ocular surface injury.

  6. Natural Proteasome Inhibitor Celastrol Suppresses Androgen-Independent Prostate Cancer Progression by Modulating Apoptotic Proteins and NF-kappaB

    E-Print Network [OSTI]

    Dai, Yao; Jeffrey, Tang; Wenhua, Xiaojie; Meng, Yang; Burstein, Ezra; Lawrence, Theodore S.; Xu, Liang

    2010-12-10

    Background Celastrol is a natural proteasome inhibitor that exhibits promising anti-tumor effects in human malignancies, especially the androgen-independent prostate cancer (AIPC) with constitutive NF-?B activation. ...

  7. Linear noise approximation of noise-induced oscillation in NF-?B signaling network

    E-Print Network [OSTI]

    Jaewook Joo

    2010-10-08

    NF-{\\kappa}B, one of key regulators of inflammation, apoptosis, and differentiation, was found to have noisy oscillatory shuttling between the nucleus and the cytoplasm in single cells when cells are stimulated by cytokine TNF{\\alpha}. We present the analytical analysis which uncovers the underlying physical mechanisms of this spectacular noise-induced transition in biological networks. Starting with the master equation describing both signaling and transcription events in NF-{\\kappa}B signaling network, we derived the macroscopic and the Fokker-Planck equations by using van Kampen's sysem size expansion. Using the noise-induced oscillatory signatures present in the power spectrum, we constructed the two-dimensional phase diagram where the noise-induced oscillation emerges in the dynamically stable parameter space.

  8. Omentin inhibits TNF-{alpha}-induced expression of adhesion molecules in endothelial cells via ERK/NF-{kappa}B pathway

    SciTech Connect (OSTI)

    Zhong, Xia, E-mail: zhongxia1977@126.com [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China); Li, Xiaonan; Liu, Fuli; Tan, Hui [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China); Shang, Deya, E-mail: wenhuashenghuo1@163.com [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)] [Department of Emergency, Provincial Hospital Affiliated to Shandong University, Jinan 250021 (China)

    2012-08-24

    Highlights: Black-Right-Pointing-Pointer Omentin inhibited TNF-{alpha}-induced adhesion of THP-1 cells to HUVECs. Black-Right-Pointing-Pointer Omentin reduces expression of ICAM-1 and VCAM-1 induced by TNF-{alpha} in HUVECs. Black-Right-Pointing-Pointer Omentin inhibits TNF-{alpha}-induced ERK and NF-{kappa}B activation in HUVECs. Black-Right-Pointing-Pointer Omentin supreeses TNF-{alpha}-induced expression of ICAM-1 and VCAM-1 via ERK/NF-{kappa}B pathway. -- Abstract: In the present study, we investigated whether omentin affected the expression of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in tumor necrosis factor-{alpha} (TNF-{alpha}) induced human umbilical vein endothelial cells (HUVECs). Our data showed that omentin decreased TNF-{alpha}-induced expression of ICAM-1 and VCAM-1 in HUVECs. In addition, omentin inhibited TNF-{alpha}-induced adhesion of THP-1 cells to HUVECs. Further, we found that omentin inhibited TNF-{alpha}-activated signal pathway of nuclear factor-{kappa}B (NF-{kappa}B) by preventing NF-{kappa}B inhibitory protein (I{kappa}B{alpha}) degradation and NF-{kappa}B/DNA binding activity. Omentin pretreatment significantly inhibited TNF-{alpha}-induced ERK activity and ERK phosphorylation in HUVECs. Pretreatment with PD98059 suppressed TNF-{alpha}-induced NF-{kappa}B activity. Omentin, NF-kB inhibitor (BAY11-7082) and ERK inhibitor (PD98059) reduced the up-regulation of ICAM-1 and VCAM-1 induced by TNF-{alpha}. These results suggest that omentin may inhibit TNF-{alpha}-induced expression of adhesion molecules in endothelial cells via blocking ERK/NF-{kappa}B pathway.

  9. Agreement Execution Process Study: CRADAs and NF-WFO Agreements and the Speed of Business

    SciTech Connect (OSTI)

    Harrer, Bruce J.; Cejka, Cheryl L.; Macklin, Richard; Miksovic, Ann

    2011-02-01

    This report summarizes the findings of a study on the execution of Cooperative Research and Development Agreements (CRADAs) and Non-Federal Work for Others (NF-WFO) agreements across the U.S. Department of Energy (DOE) laboratory complex. The study provides quantitiative estimates of times required to negotiate and execute these agreements across the DOE complex. It identifies factors impacting on cycle times and describes best practicies used at various laboratories and site offices that reduce cycle times.

  10. MC/NF TARGET SHIELDING STUDIES. NICHOLAS SOUCHLAS (10/29/2010)

    E-Print Network [OSTI]

    McDonald, Kirk

    MC/NF TARGET SHIELDING STUDIES. NICHOLAS SOUCHLAS (10/29/2010) 1 #12;Energy deposition from MARS, MARS+MCNP codes. STANDARD (STUDY II) GEOMETRY. STANDARD SHIELDING (80%WC+20% H2O). 4MW proton beamW to 50 kW in total power. 4 24 GeV 8 GeV #12;OFF/ON SHIELDING, DIFFERENT NEUTRON ENERGY CUTOFFS. 5 #12

  11. 1 Managed by UT-Battelle for the U.S. Department of Energy NF/IDS Hg Vessel Layout 30 Jun 09

    E-Print Network [OSTI]

    McDonald, Kirk

    1 Managed by UT-Battelle for the U.S. Department of Energy NF/IDS Hg Vessel Layout 30 Jun 09;3 Managed by UT-Battelle for the U.S. Department of Energy NF/IDS Hg Vessel Layout 30 Jun 09 Cross Section to flow out the front of the cryostat #12;4 Managed by UT-Battelle for the U.S. Department of Energy NF

  12. IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverse (JournalvivoHighHussein KhalilResearch &ENERGYWhoCELL WALLS4,IDRMSIDS-NF

  13. FY13 Summary Report on the Augmentation of the Spent Fuel Composition Dataset for Nuclear Forensics: SFCOMPO/NF

    SciTech Connect (OSTI)

    Brady Raap, Michaele C.; Lyons, Jennifer A.; Collins, Brian A.; Livingston, James V.

    2014-03-31

    This report documents the FY13 efforts to enhance a dataset of spent nuclear fuel isotopic composition data for use in developing intrinsic signatures for nuclear forensics. A review and collection of data from the open literature was performed in FY10. In FY11, the Spent Fuel COMPOsition (SFCOMPO) excel-based dataset for nuclear forensics (NF), SFCOMPO/NF was established and measured data for graphite production reactors, Boiling Water Reactors (BWRs) and Pressurized Water Reactors (PWRs) were added to the dataset and expanded to include a consistent set of data simulated by calculations. A test was performed to determine whether the SFCOMPO/NF dataset will be useful for the analysis and identification of reactor types from isotopic ratios observed in interdicted samples.

  14. ABD56 causes osteoclast apoptosis by inhibiting the NF{kappa}B and ERK pathways

    SciTech Connect (OSTI)

    Idris, Aymen [Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh, EH4 2XU (United Kingdom); Mrak, Emanuela [Bone Metabolic Unit, Scientific Institute H San Raffaele, via Olgettina 60, 20132 Milano (Italy); Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milano, via Vanvitelli 32, 20129 Milano (Italy); Greig, Iain [Department of Medicine and Therapeutics, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD (United Kingdom); Guidobono, Francesca [Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milano, via Vanvitelli 32, 20129 Milano (Italy); Ralston, Stuart H. [Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh, EH4 2XU (United Kingdom); Hof, Rob van 't [Rheumatic Diseases Unit, Molecular Medicine Centre, University of Edinburgh, General Western Hospital, Edinburgh, EH4 2XU (United Kingdom)], E-mail: rvanthof@staffmail.ed.ac.uk

    2008-06-20

    We have previously shown that the biphenylcarboxylic acid butanediol ester (ABD56) inhibits osteoclast formation and activity in vitro and in vivo. However, the mechanism of action of this compound is unknown. ABD56 inhibited osteoclast formation and caused osteoclast apoptosis, but had no effects on osteoblasts or macrophages. As the NF{kappa}B and MAPK pathways are essential for osteoclast formation and survival, we studied the effects of ABD56 on these pathways. ABD56 caused phosphorylation of p38, JNK and nuclear translocation of c-jun in osteoclasts. ABD56-induced apoptosis was prevented by the caspase inhibitor zVAD-fmk but was not prevented by the p38- or JNK-inhibitors. ABD56 completely abolished RANKL-induced I{kappa}B and ERK1/2 phosphorylation. Increasing the amount of RANKL partially rescued ABD56-induced apoptosis, indicating that the apoptosis is most probably due to the inhibition of survival signals such as ERK and NF{kappa}B, rather than activation of the p38 or Jnk MAPK pathways.

  15. Journal of Membrane Science 256 (2005) 134142 Characterization of novel acid-stable NF membranes before and after

    E-Print Network [OSTI]

    Freger, Viatcheslav "Slava"

    2005-01-01

    to hot 20% sulfuric acid solutions for various times. Swelling and morphological changes measured Nanofiltration (NF) membranes represent a class of mem- branes with capabilities to selectively separate low- sulfone, which support an ultra-thin selective layer a few tens to a few hundred of nanometers thick

  16. Mast cell chymase induces smooth muscle cell apoptosis by disrupting NF-{kappa}B-mediated survival signaling

    SciTech Connect (OSTI)

    Leskinen, Markus J. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Heikkilae, Hanna M. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Speer, Mei Y. [Bioengineering Department, University of Washington, Seattle, WA 98195 (United States); Hakala, Jukka K. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Laine, Mika [Department of Medicine, Helsinki University Central Hospital and Minerva Institute for Medical Research, Helsinki (Finland); Kovanen, Petri T. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland); Lindstedt, Ken A. [Wihuri Research Institute, Kalliolinnantie 4, FI-00140 Helsinki (Finland)]. E-mail: ken.lindstedt@wri.fi

    2006-05-01

    Chymase released from activated mast cells induces apoptosis of vascular smooth muscle cells (SMCs) in vitro by degrading the pericellular matrix component fibronectin, so causing disruption of focal adhesion complexes and Akt dephosphorylation, which are necessary for cell adhesion and survival. However, the molecular mechanisms of chymase-mediated apoptosis downstream of Akt have remained elusive. Here, we show by means of RT-PCR, Western blotting, EMSA, immunocytochemistry and confocal microscopy, that chymase induces SMC apoptosis by disrupting NF-{kappa}B-mediated survival signaling. Following chymase treatment, the translocation of active NF-{kappa}B/p65 to the nucleus was partly abolished and the amount of nuclear p65 was reduced. Pretreatment of SMCs with chymase also inhibited LPS- and IL-1{beta}-induced nuclear translocation of p65. The chymase-induced degradation of p65 was mediated by active caspases. Loss of NF-{kappa}B-mediated transactivation resulted in downregulation of bcl-2 mRNA and protein expression, leading to mitochondrial swelling and release of cytochrome c. The apoptotic process involved activation of both caspase 9 and caspase 8. The results reveal that, by disrupting the NF-{kappa}B-mediated survival-signaling pathway, activated chymase-secreting mast cells can mediate apoptosis of cultured arterial SMCs. Since activated mast cells colocalize with apoptotic SMCs in vulnerable areas of human atherosclerotic plaques, they may participate in the weakening and rupture of atherosclerotic plaques.

  17. Genome-wide scan reveals association of psoriasis with IL-23 and NF-kB pathways

    E-Print Network [OSTI]

    Abecasis, Goncalo

    Genome-wide scan reveals association of psoriasis with IL-23 and NF-kB pathways Rajan P Nair1 Abecasis4,20, for the Collaborative Association Study of Psoriasis18 Psoriasis is a common immune-mediated disorder that affects the skin, nails and joints. To identify psoriasis susceptibility loci, we genotyped

  18. Modeling green fluorescent protein transcription, translation and modification as a method to obtain NF-kappaB activation profiles 

    E-Print Network [OSTI]

    Laible, Allyson Marie

    2009-05-15

    that fluorescence increases up to 24 hours after an initial delay of approximately four hours. The fluorescence data was also used to develop a model describing significant events leading to NF-?B activation and GFP expression. In addition, a model describing...

  19. Decreasing NF-?B Expression Enhances Odontoblastic Differentiation and Collagen Expression in Dental Pulp Stem Cells Exposed to Inflammatory Cytokines 

    E-Print Network [OSTI]

    Hozhabri, Neda S. T.; Benson, M. Douglas; Vu, Michael D.; Patel, Rinkesh H.; Martinez, Rebecca M.; Nakhaie, Fatemeh N.; Kim, Harry K. W.; Varanasi, Venu G.

    2015-01-28

    Decreasing NF-?B Expression Enhances Odontoblastic Differentiation and Collagen Expression in Dental Pulp Stem Cells Exposed to Inflammatory Cytokines Neda S. T. Hozhabri1, M. Douglas Benson1, Michael D. Vu1, Rinkesh H. Patel1, Rebecca M. Martinez1, Fatemeh N...

  20. Ascorbic acid suppresses endotoxemia and NF-?B signaling cascade in alcoholic liver fibrosis in guinea pigs: A mechanistic approach

    SciTech Connect (OSTI)

    Abhilash, P.A.; Harikrishnan, R.; Indira, M.

    2014-01-15

    Alcohol consumption increases the small intestinal bacterial overgrowth (SIBO) and intestinal permeability of endotoxin. The endotoxin mediated inflammatory signaling plays a major role in alcoholic liver fibrosis. We evaluated the effect of ascorbic acid (AA), silymarin and alcohol abstention on the alcohol induced endotoxemia and NF-?B activation cascade pathway in guinea pigs (Cavia porcellus). Guinea pigs were administered ethanol at a daily dose of 4 g/kg b.wt for 90 days. After 90 days, ethanol administration was stopped. The ethanol treated animals were divided into abstention, silymarin (250 mg/kg b.wt) and AA (250 mg/kg b.wt) supplemented groups and maintained for 30 days. The SIBO, intestinal permeability and endotoxin were significantly increased in the ethanol group. The mRNA expressions of intestinal proteins claudin, occludin and zona occludens-1 were significantly decreased in ethanol group. The mRNA levels of inflammatory receptors, activity of IKK? and the protein expressions of phospho-I?B?, NF-?B, TNF-?, TGF-?{sub 1} and IL-6 were also altered in ethanol group. The expressions of fibrosis markers ?-SMA, ?{sub 1} (I) collagen and sirius red staining in the liver revealed the induction of fibrosis. But the supplementation of AA could induce greater reduction of ethanol induced SIBO, intestinal barrier defects, NF-?B activation and liver fibrosis than silymarin. The possible mechanism may be the inhibitory effect of AA on SIBO, intestinal barrier defect and IKK?, which decreased the activation of NF-?B and synthesis of cytokines. This might have led to suppression of HSCs activation and liver fibrosis. - Highlights: • Alcohol increases intestinal bacterial overgrowth and permeability of endotoxin. • Endotoxin mediated inflammation plays a major role in alcoholic liver fibrosis. • Ascorbic acid reduces endotoxemia, NF-?B activation and proinflammatory cytokines. • AA's action is by inhibition of SIBO, IKK? and alteration of intestinal permeability. • This might have led to suppression of HSCs activation and liver fibrosis.

  1. Thermal Reactions of Uranium Metal, UO2, U3O8, UF4, and UO2F2 with NF3 to Produce UF6

    SciTech Connect (OSTI)

    McNamara, Bruce K.; Scheele, Randall D.; Kozelisky, Anne E.; Edwards, Matthew K.

    2009-11-01

    he objective of this paper is to demonstrate that NF3 fluorinates uranium metal, UO2, UF4, UO3, U3O8, and UO2F2•2H2O to produce the volatile UF6 at temperatures between 100 and 500?C. Thermogravimetric reaction profiles are described that reflect changes in the uranium oxidation state and discrete chemical speciation. Differences in the onset temperatures for each system indicate that NF3-substrate interactions are important for the temperature at which NF3 reacts: U metal > UO3 > UO2 > UO2F2 > UF4 and in fact may indicate different fluorination mechanisms for these various substrates. These studies demonstrate that NF3 is a potential replacement fluorinating agent in the existing nuclear fuel cycle and in oft-proposed actinide volatility reprocessing.

  2. TCR and CD28 activate the transcription factor NF- B in T-cells viadistinct adaptor signaling complexes

    E-Print Network [OSTI]

    Thaker, Youg Raj; Schneider, Helga; Rudd, Christopher E.

    2014-10-23

    Article history: Received 19 A Received in re Accepted 15 O Available onlin Keywords: Adaptors ADAP VAV-1 GRB-2 NF-?B ed fo nd CD ng T- n wit ?B in F-?B p?/? m Over-expression of ADAP or its binding partner SKAP1 failed to enhance anti-CD28... to CD28 addition, Tu can deliver complexes co-operate Rac-1 and m Despite ponents of T-cells have various kno 170F) mice that the TC the NF-?B p that the CD ent signalin 2. Materia 2.1. Mice an DO11.10 (kindly pro School of M Dr. Erik Pe housed und...

  3. Nmero Nome 1T 2T R1T R2T E AC NF 42200 Armando Dias

    E-Print Network [OSTI]

    Baia, Margarida

    Sheet1 Page 1 Número Nome 1T 2T R1T R2T E AC NF 42200 Armando Dias 53515 Jorge Branco 55774 João 70100 Catarina Ferreira 70146 Mariana Castro 70364 Nuno Gaspar 70524 António Grego 10 2 5 70934 João.2 2 15 78167 Afonso Sarmento 13.8 8.3 7.6 2 12 78175 Carlos Martins 14.3 12.6 11 2 14 78183 Ana

  4. Etching of UO{sub 2} in NF{sub 3} RF Plasma Glow Discharge

    SciTech Connect (OSTI)

    John M. Veilleux

    1999-08-01

    A series of room temperature, low pressure (10.8 to 40 Pa), low power (25 to 210 W) RF plasma glow discharge experiments with UO{sub 2} were conducted to demonstrate that plasma treatment is a viable method for decontaminating UO{sub 2} from stainless steel substrates. Experiments were conducted using NF{sub 3} gas to decontaminate depleted uranium dioxide from stainless-steel substrates. Depleted UO{sub 2} samples each containing 129.4 Bq were prepared from 100 microliter solutions of uranyl nitrate hexahydrate solution. The amorphous UO{sub 2} in the samples had a relatively low density of 4.8 gm/cm{sub 3}. Counting of the depleted UO{sub 2} on the substrate following plasma immersion was performed using liquid scintillation counting with alpha/beta discrimination due to the presence of confounding beta emitting daughter products, {sup 234}Th and {sup 234}Pa. The alpha emission peak from each sample was integrated using a gaussian and first order polynomial fit to improve quantification. The uncertainties in the experimental measurement of the etched material were estimated at about {+-} 2%. Results demonstrated that UO{sub 2} can be completely removed from stainless-steel substrates after several minutes processing at under 200 W. At 180 W and 32.7 Pa gas pressure, over 99% of all UO{sub 2} in the samples was removed in just 17 minutes. The initial etch rate in the experiments ranged from 0.2 to 7.4 {micro}m/min. Etching increased with the plasma absorbed power and feed gas pressure in the range of 10.8 to 40 Pa. A different pressure effect on UO{sub 2} etching was also noted below 50 W in which etching increased up to a maximum pressure, {approximately}23 Pa, then decreased with further increases in pressure.

  5. A (P)HMC algorithm for N_f = 2 + 1 + 1 flavours of twisted mass fermions

    E-Print Network [OSTI]

    T. Chiarappa; R. Frezzotti; C. Urbach

    2005-09-27

    We present a detailed design of a (P)HMC simulation algorithm for N_f = 2 + 1 + 1 maximally twisted Wilson quark flavours. The algorithm retains even/odd and mass-shift preconditionings combined with multiple Molecular Dynamics time scales for both the light mass degenerate, u and d, quarks and the heavy mass non-degenerate, s and c, quarks. Various non-standard aspects of the algorithm are discussed, among which those connected to the use of a polynomial approximation for the inverse (square root of the squared) Dirac matrix in the s and c quark sector.

  6. Hadron-Hadron Interactions from $N_f=2+1+1$ Lattice QCD: isospin-2 $??$ scattering length

    E-Print Network [OSTI]

    C. Helmes; C. Jost; B. Knippschild; C. Liu; J. Liu; L. Liu; C. Urbach; M. Ueding; Z. Wang; M. Werner

    2015-06-01

    We present results for the $I=2$ $\\pi\\pi$ scattering length using $N_f=2+1+1$ twisted mass lattice QCD for three values of the lattice spacing and a range of pion mass values. Due to the use of Laplacian Heaviside smearing our statistical errors are reduced compared to previous lattice studies. A detailed investigation of systematic effects such as discretisation effects, volume effects, and pollution of excited and thermal states is performed. After extrapolation to the physical point using chiral perturbation theory at NLO we obtain $M_\\pi a_0=-0.0442(2)_\\mathrm{stat}(^{+4}_{-0})_\\mathrm{sys}$.

  7. Determination of c_sw in N_f=3+1 Lattice QCD with massive Wilson fermions

    E-Print Network [OSTI]

    Patrick Fritzsch; Rainer Sommer; Felix Stollenwerk; Ulli Wolff

    2015-01-27

    We develop a strategy for the non-perturbative determination of the O(a)-improvement coefficient c_sw for Wilson fermions with massive sea quarks. The improvement condition is defined via the PCAC relation in the Schr\\"odinger functional. It is imposed along a line of constant physics designed to be close to the correct mass of the charm quark. The numerical work uses the tree-level improved L\\"uscher-Weisz gauge action in N_f=3+1 Lattice QCD.

  8. Angiotensin II modulates interleukin-1{beta}-induced inflammatory gene expression in vascular smooth muscle cells via interfering with ERK-NF-{kappa}B crosstalk

    SciTech Connect (OSTI)

    Xu, Shanqin [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States)] [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States); Zhi, Hui [Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (United States)] [Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (United States); Hou, Xiuyun [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States)] [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States); Jiang, Bingbing, E-mail: bjiang1@rics.bwh.harvard.edu [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States) [Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA (United States); Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (United States)

    2011-07-08

    Highlights: {yields} We examine how angiotensin II modulates ERK-NF-{kappa}B crosstalk and gene expression. {yields} Angiotensin II suppresses IL-1{beta}-induced prolonged ERK and NF-{kappa}B activation. {yields} ERK-RSK1 signaling is required for IL-1{beta}-induced prolonged NF-{kappa}B activation. {yields} Angiotensin II modulates NF-{kappa}B responsive genes via regulating ERK-NF-{kappa}B crosstalk. {yields} ERK-NF-{kappa}B crosstalk is a novel mechanism regulating inflammatory gene expression. -- Abstract: Angiotensin II is implicated in cardiovascular diseases, which is associated with a role in increasing vascular inflammation. The present study investigated how angiotensin II modulates vascular inflammatory signaling and expression of inducible nitric oxide synthase (iNOS) and vascular cell adhesion molecule (VCAM)-1. In cultured rat aortic vascular smooth muscle cells (VSMCs), angiotensin II suppressed interleukin-1{beta}-induced prolonged phosphorylation of extracellular signal-regulated kinase (ERK) and ribosomal S6 kinase (RSK)-1, and nuclear translocation of nuclear factor (NF)-{kappa}B, leading to decreased iNOS but enhanced VCAM-1 expression, associated with an up-regulation of mitogen-activated protein kinase phosphatase-1 expression. Knock-down of RSK1 selectively down regulated interleukin-1{beta}-induced iNOS expression without influencing VCAM-1 expression. In vivo experiments showed that interleukin-1{beta}, iNOS, and VCAM-1 expression were detectable in the aortic arches of both wild-type and apolipoprotein E-deficient (ApoE{sup -/-}) mice. VCAM-1 and iNOS expression were higher in ApoE{sup -/-} than in wild type mouse aortic arches. Angiotensin II infusion (3.2 mg/kg/day, for 6 days, via subcutaneous osmotic pump) in ApoE{sup -/-} mice enhanced endothelial and adventitial VCAM-1 and iNOS expression, but reduced medial smooth muscle iNOS expression associated with reduced phosphorylation of ERK and RSK-1. These results indicate that angiotensin II can differentially modulate inflammatory gene expression in aortic smooth muscle cells through influencing ERK-NF-{kappa}B crosstalk, which may contribute to angiotensin II-induced inflammatory disorders related to cardiovascular diseases.

  9. Apolipoprotein E inhibits osteoclast differentiation via regulation of c-Fos, NFATc1 and NF-?B

    SciTech Connect (OSTI)

    Kim, Woo-Shin; Kim, Hyung Joon; Lee, Zang Hee [Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749 (Korea, Republic of); Lee, Youngkyun [Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu 700-422 (Korea, Republic of); Kim, Hong-Hee, E-mail: hhbkim@snu.ac.kr [Department of Cell and Developmental Biology, BK21 Program and Dental Research Institute, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749 (Korea, Republic of)

    2013-02-15

    Apolipoprotein E (ApoE) plays a major role in the transport and metabolism of lipid. Other functions of ApoE include modulation of innate and adaptive immune responses. The expression of ApoE in osteoblasts and its relevance with bone formation have also been reported. However, the effect of ApoE on osteoclasts has not yet been examined. Here, we investigated the role of ApoE in osteoclast differentiation using bone marrow-derived macrophages (BMMs) and RAW264.7 cells. We found a down-regulation of ApoE gene expression during osteoclastic differentiation of those cells. Overexpression of ApoE in BMMs and RAW264.7 cells significantly blocked the induction of c-Fos and nuclear factor of activated T cell c1 (NFATc1), transcription factors critical for expression of osteoclast marker genes, by receptor activator of nuclear factor ?B ligand (RANKL), the osteoclast differentiation factor. ApoE inhibited osteoclast differentiation, as measured by decreased number of tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (MNCs). In addition, ApoE reduced the expression of dendritic cell-specific transmembrane protein (DC-STAMP) and ATPase, H{sup +} transporting, lysosomal 38 kDa, V0 subunit d2 (ATP6v0d2), genes involved in cell–cell fusion during osteoclastogenesis. Knock-down of ApoE using a specific siRNA promoted the RANKL-mediated induction of osteoclast differentiation. While ApoE did not affect the activation of ERK, JNK, and p38 MAPK signaling pathways by RANKL, the phosphorylation of p65 trans-activation domain on serine 536 and transcription activity of NF-?B were reduced by ApoE overexpression. These findings suggest that ApoE plays an inhibitory role in osteoclast differentiation via the suppression of RANKL-dependent activation of NF-?B and induction of c-Fos and NFATc1. - Highlights: ? Apolipoprotein E (ApoE) significantly inhibited osteoclast differentiation and activation of NF-?B. ? ApoE decreased the induction of osteoclast marker genes such as c-Fos, NFATc1, TRAP, DC-STAMP and Cathepsin K. ? ApoE reduced number of TRAP-positive multinuclear cells. ? Exogenous ApoE inhibits osteoclast formation by reduction of crucial factor of osteoclast differentiation. ? knock-down of ApoE promoted the RANKL-mediated induction of osteoclast differentiation.

  10. Differential Effects of ?-catenin and NF-?B Interplay in the Regulation of Cell Proliferation, Inflammation and Tumorigenesis in Response to Bacterial Infection

    E-Print Network [OSTI]

    Chandrakesan, Parthasarathy; Jakkula, Laxmi Uma Maheswar Rao; Ahmed, Ishfaq; Roy, Badal; Anant, Shrikant; Umar, Shahid

    2013-11-21

    Both ?-catenin and NF-?B have been implicated in our laboratory as candidate factors in driving proliferation in an in vivo model of Citrobacter rodentium (CR)-induced colonic crypt hyper-proliferation and hyperplasia. Herein, we test the hypothesis...

  11. GRP94/gp96 Elicits ERK Activation in Murine Macrophages A ROLE FOR ENDOTOXIN CONTAMINATION IN NF-B ACTIVATION AND NITRIC OXIDE PRODUCTION*

    E-Print Network [OSTI]

    Nicchitta, Chris

    GRP94/gp96 Elicits ERK Activation in Murine Macrophages A ROLE FOR ENDOTOXIN CONTAMINATION IN NF- B signaling and ni- tric oxide production, as well as the MAP kinase p38, JNK, and ERK signaling cascades a marked increase in ERK phosphorylation at protein concentrations as low as 2 g/ml. These results

  12. Osthole inhibits the invasive ability of human lung adenocarcinoma cells via suppression of NF-?B-mediated matrix metalloproteinase-9 expression

    SciTech Connect (OSTI)

    Kao, Shang-Jyh [Department of Chest Medicine, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan (China) [Department of Chest Medicine, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan (China); School of Respiratory Therapy, Taipei Medical University, Taipei Taiwan (China); Su, Jen-Liang [Graduate Institute of Cancer Biology, College of Medicine, China Medical University, Taichung, Taiwan (China) [Graduate Institute of Cancer Biology, College of Medicine, China Medical University, Taichung, Taiwan (China); Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan (China); Department of Biotechnology, Asia University, Taichung, Taiwan (China); Chen, Chi-Kuan [Graduate Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China)] [Graduate Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan (China); Yu, Ming-Chih; Bai, Kuan-Jen; Chang, Jer-Hua [Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan (China)] [Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan (China); Bien, Mauo-Ying [School of Respiratory Therapy, Taipei Medical University, Taipei Taiwan (China) [School of Respiratory Therapy, Taipei Medical University, Taipei Taiwan (China); Division of Pulmonary Medicine, Department of Internal Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan (China); Yang, Shun-Fa [Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan (China) [Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan (China); Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan (China); Chien, Ming-Hsien, E-mail: mhchien1976@gmail.com [Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan (China)] [Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan (China)

    2012-05-15

    The induction of matrix metalloproteinase (MMP)-9 is particularly important for the invasiveness of various cancer cells. Osthole, a natural coumarin derivative extracted from traditional Chinese medicines, is known to inhibit the proliferation of a variety of tumor cells, but the effect of osthole on the invasiveness of tumor cells is largely unknown. This study determines whether and by what mechanism osthole inhibits invasion in CL1-5 human lung adenocarcinoma cells. Herein, we found that osthole effectively inhibited the migratory and invasive abilities of CL1-5 cells. A zymographic assay showed that osthole inhibited the proteolytic activity of MMP-9 in CL1-5 cells. Inhibition of migration, invasion, and MMP2 and/or MMP-9 proteolytic activities was also observed in other lung adenocarcinoma cell lines (H1299 and A549). We further found that osthole inhibited MMP-9 expression at the messenger RNA and protein levels. Moreover, a chromatin immunoprecipitation assay showed that osthole inhibited the transcriptional activity of MMP-9 by suppressing the DNA binding activity of nuclear factor (NF)-?B in the MMP-9 promoter. Using reporter assays with point-mutated promoter constructs further confirmed that the inhibitory effect of osthole requires an NF-?B binding site on the MMP-9 promoter. Western blot and immunofluorescence assays demonstrated that osthole inhibited NF-?B activity by inhibiting I?B-? degradation and NF-?B p65 nuclear translocation. In conclusion, we demonstrated that osthole inhibits NF-?B-mediated MMP-9 expression, resulting in suppression of lung cancer cell invasion and migration, and osthole might be a potential agent for preventing the invasion and metastasis of lung cancer. -- Highlights: ? Osthole treatment inhibits lung adenocarcinoma cells migration and invasion. ? Osthole reduces the expression and proteolytic activity of MMP-9. ? Osthole inhibits MMP-9 transcription via suppression of NF-?B binding activity. ? Osthole inhibits I?B? degradation and NF-?B nucleus translocation. ? Osthole suppresses EMT by repressing vimentin and inducing E-cadherin expression.

  13. Integrated Genomic Analysis Identifies Clinically Relevant Subtypes of Glioblastoma Characterized by Abnormalities in PDGFRA, IDH1, EGFR, and NF1

    SciTech Connect (OSTI)

    Verhaak, Roel GW; Hoadley, Katherine A; Purdom, Elizabeth; Wang, Victoria; Qi, Yuan; Wilkerson, Matthew D; Miller, C Ryan; Ding, Li; Golub, Todd; Mesirov, Jill P; Alexe, Gabriele; Lawrence, Michael; O'Kelly, Michael; Tamayo, Pablo; Weir, Barbara A; Gabriel, Stacey; Winckler, Wendy; Gupta, Supriya; Jakkula, Lakshmi; Feiler, Heidi S; Hodgson, J Graeme; James, C David; Sarkaria, Jann N; Brennan, Cameron; Kahn, Ari; Spellman, Paul T; Wilson, Richard K; Speed, Terence P; Gray, Joe W; Meyerson, Matthew; Getz, Gad; Perou, Charles M; Hayes, D Neil; Network, The Cancer Genome Atlas Research

    2009-09-03

    The Cancer Genome Atlas Network recently cataloged recurrent genomic abnormalities in glioblastoma multiforme (GBM). We describe a robust gene expression-based molecular classification of GBM into Proneural, Neural, Classical, and Mesenchymal subtypes and integrate multidimensional genomic data to establish patterns of somatic mutations and DNA copy number. Aberrations and gene expression of EGFR, NF1, and PDGFRA/IDH1 each define the Classical, Mesenchymal, and Proneural subtypes, respectively. Gene signatures of normal brain cell types show a strong relationship between subtypes and different neural lineages. Additionally, response to aggressive therapy differs by subtype, with the greatest benefit in the Classical subtype and no benefit in the Proneural subtype. We provide a framework that unifies transcriptomic and genomic dimensions for GBM molecular stratification with important implications for future studies.

  14. Simulating QCD at the Physical Point with $N_f=2$ Wilson Twisted Mass Fermions at Maximal Twist

    E-Print Network [OSTI]

    ETM Collaboration; A. Abdel-Rehim; C. Alexandrou; F. Burger; M. Constantinou; P. Dimopoulos; R. Frezzotti; K. Hadjiyiannakou; K. Jansen; C. Kallidonis; B. Kostrzewa; G. Koutsou; M. Mangin-Brinet; M. Petschlies; G. Pientka; G. C. Rossi; C. Urbach; U. Wenger

    2015-07-17

    We present simulations of QCD using Nf=2 dynamical Wilson twisted mass lattice QCD with physical value of the pion mass and at one value of the lattice spacing. Such simulations at ~0.09 fm became possible by adding the clover term to the action. While O(a) improvement is still guaranteed by Wilson twisted mass fermions at maximal twist, the introduction of the clover term reduces cutoff effects related to isospin symmetry breaking. We give results for a set of phenomenologically interesting observables like pseudo-scalar masses and decay constants, quark masses and the anomalous magnetic moments of leptons. We mostly find remarkably good agreement with phenomenology, even though we cannot take the continuum and thermodynamic limits.

  15. Update on a short-distance D^0-meson mixing calculation with $N_f=2+1$ flavors

    E-Print Network [OSTI]

    C. C. Chang; C. Bernard; C. M. Bouchard; A. X. El-Khadra; E. D. Freeland; E. Gámiz; A. S. Kronfeld; J. Laiho; R. S. Van de Water

    2014-12-13

    We present an update on our calculation of the short-distance $D^0$-meson mixing hadronic matrix elements. The analysis is performed on the MILC collaboration's $N_f=2+1$ asqtad configurations. We use asqtad light valence quarks and the Sheikoleslami-Wohlert action with the Fermilab interpretation for the valence charm quark. SU(3), partially quenched, rooted, staggered heavy-meson chiral perturbation theory is used to extrapolate to the chiral-continuum limit. Systematic errors arising from the chiral-continuum extrapolation, heavy-quark discretization, and quark-mass uncertainties are folded into the statistical errors from the chiral-continuum fits with methods of Bayesian inference. A preliminary error budget for all five operators is presented.

  16. Metastatic function of BMP-2 in gastric cancer cells: The role of PI3K/AKT, MAPK, the NF-{kappa}B pathway, and MMP-9 expression

    SciTech Connect (OSTI)

    Kang, Myoung Hee [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Oh, Sang Cheul [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Lee, Hyun Joo [Department of Pathology, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Department of Pathology, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Kang, Han Na; Kim, Jung Lim [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Graduate School of Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Kim, Jun Suk [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)] [Division of Oncology/Hematology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of); Yoo, Young A., E-mail: ydanbi@korea.ac.kr [Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Korea University, Seoul 136-705 (Korea, Republic of)

    2011-07-15

    Bone morphogenetic proteins (BMPs) have been implicated in tumorigenesis and metastatic progression in various types of cancer cells, but the role and cellular mechanism in the invasive phenotype of gastric cancer cells is not known. Herein, we determined the roles of phosphoinositide 3-kinase (PI3K)/AKT, extracellular signal-regulated protein kinase (ERK), nuclear factor (NF)-{kappa}B, and matrix metalloproteinase (MMP) expression in BMP-2-mediated metastatic function in gastric cancer. We found that stimulation of BMP-2 in gastric cancer cells enhanced the phosphorylation of AKT and ERK. Accompanying activation of AKT and ERK kinase, BMP-2 also enhanced phosphorylation/degradation of I{kappa}B{alpha} and the nuclear translocation/activation of NF-{kappa}B. Interestingly, blockade of PI3K/AKT and ERK signaling using LY294002 and PD98059, respectively, significantly inhibited BMP-2-induced motility and invasiveness in association with the activation of NF-{kappa}B. Furthermore, BMP-2-induced MMP-9 expression and enzymatic activity was also significantly blocked by treatment with PI3K/AKT, ERK, or NF-{kappa}B inhibitors. Immunohistochemistry staining of 178 gastric tumor biopsies indicated that expression of BMP-2 and MMP-9 had a significant positive correlation with lymph node metastasis and a poor prognosis. These results indicate that the BMP-2 signaling pathway enhances tumor metastasis in gastric cancer by sequential activation of the PI3K/AKT or MAPK pathway followed by the induction of NF-{kappa}B and MMP-9 activity, indicating that BMP-2 has the potential to be a therapeutic molecular target to decrease metastasis.

  17. Nuclear NF-?B Expression Correlates With Outcome Among Patients With Head and Neck Squamous Cell Carcinoma Treated With Primary Chemoradiation Therapy

    SciTech Connect (OSTI)

    Balermpas, Panagiotis [Department of Radiation Therapy and Oncology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany); Michel, Yvonne [Senckenberg Institute of Pathology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)] [Senckenberg Institute of Pathology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany); Wagenblast, Jens [Department of Otorhinolaryngology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)] [Department of Otorhinolaryngology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany); Seitz, Oliver [Department of Maxillofacial Surgery, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)] [Department of Maxillofacial Surgery, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany); Sipek, Florian; Rödel, Franz; Rödel, Claus [Department of Radiation Therapy and Oncology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany); Fokas, Emmanouil, E-mail: emmanouil.fokas@kgu.de [Department of Radiation Therapy and Oncology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe – University Frankfurt am Main, Frankfurt (Germany)

    2013-07-15

    Background: To examine whether nuclear NF-?B expression correlates with outcome in patients with head and neck squamous cell carcinoma (HNSCC) treated with primary chemoradiation therapy (CRT). Methods and Materials: Between 2007 and 2010, 101 patients with locally advanced primary HNSCC were treated with definitive simultaneous CRT. Pretreatment biopsy specimens were analyzed for NF-?B p65 (RelA) nuclear immunoreactivity. A sample was assigned to be positive with more than 5% positive nuclear expression. The predictive relevance of NF-?B and clinicopathologic factors for overall survival (OS), progression-free survival (PFS), local progression-free survival (LPFS), and metastasis-free survival (DMFS) was examined by univariate and multivariate analysis. Results: No significant differences between the groups were observed with regard to age, sex, total radiation dose, fractionation mode, total chemotherapy applied, T stage or grading. Patients with p65 nuclear positive biopsy specimens showed significantly a higher rate of lymph node metastasis (cN2c or cN3 status, P=.034). Within a mean follow-up time of 25 months (range, 2.33-62.96 months) OS, PFS, and DMFS were significantly poorer in the p65 nuclear positive group (P=.008, P=.027, and P=.008, respectively). These correlations remained significant in multivariate analysis. Conclusion: NF-?B/p65 nuclear expression is associated with increased lymphatic and hematogenous tumor dissemination and decreased survival in HNSCC patients treated with primary CRT. Our results may foster further investigation of a predictive relevance of NF-?B/p65 and its role as a suitable target for a molecular-based targeted therapy in HNSCC cancer.

  18. Inhibition of inflammatory mediators contributes to the anti-inflammatory activity of KYKZL-1 via MAPK and NF-?B pathway

    SciTech Connect (OSTI)

    Xu, Guang-Lin [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Department of Pharmacology, University of Michigan, Ann Arbor (United States); Du, Yi-Fang; Cheng, Jing; Huan, Lin [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Chen, Shi-Cui [Jinhu Food and Drug Administration, Jiangsu (China); Wei, Shao-Hua [College of Chemistry and Materials Science, Nanjing Normal University, Nanjing (China); Gong, Zhu-Nan, E-mail: biopharmacology@126.com [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Cai, Jie; Qiu, Ting; Wu, Hao; Sun, Ting [Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing (China); Ao, Gui-Zhen [Department of Medicinal Chemistry, School of Pharmacy, Soochow University, Jiangsu (China)

    2013-10-01

    KYKZL-1, a newly synthesized compound with COX/5-LOX dual inhibition, was subjected to the anti-inflammatory activity test focusing on its modulation of inflammatory mediators as well as intracellular MAPK and NF-?B signaling pathways. In acute ear edema model, pretreatment with KYKZL-1 (p.o.) dose-dependently inhibited the xylene-induced ear edema in mice with a higher inhibition than diclofenac. In a three-day TPA-induced inflammation, KYKZL-1 also showed significant anti-inflammatory activity with inhibition ranging between 20% and 64%. In gastric lesion test, KYKZL-1 elicited markedly fewer stomach lesions with a low index of ulcer as compared to diclofenac in rats. In further studies, KYKZL-1 was found to significantly inhibit the production of NO, PGE{sub 2}, LTB{sub 4} in LPS challenged RAW264.7, which is parallel to its attenuation of the expression of iNOS, COX-2, 5-LOX mRNAs or proteins and inhibition of phosphorylation of p38 and ERK MAPKs and activation of NF-?B. Taken together, our data indicate that KYKZL-1 comprises dual inhibition of COX and 5-LOX and exerts an obvious anti-inflammatory activity with an enhanced gastric safety profile via simultaneous inhibition of phosphorylation of p38 and ERK MAPKs and activation of NF-?B. - Highlights: • KYKZL-1 is designed to exhibit COX/5-LOX dual inhibition. • KYKZL-1 inhibits NO, PGE{sub 2} and LTB{sub 4} and iNOS, COX-2 and 5-LOX mRNAs and MAPKs. • KYKZL-1 inhibits phosphorylation of MAPKs. • KYKZL-1 inactivates NF-?B pathway.

  19. D O M A I NF A L L 2 0 0 4 55555 How a Top Secret Satellite and Fast-Thinking

    E-Print Network [OSTI]

    D O M A I NF A L L 2 0 0 4 55555 How a Top Secret Satellite and Fast-Thinking Navy and Air Force man in the Navy who had worked directly with the top secret Corona Air Force spy satellite, knew what the weather at the Pacific Ocean splashdown site. "When I arrived in Hawaii, I still had my top secret

  20. Moderate extracellular acidification inhibits capsaicin-induced cell death through regulating calcium mobilization, NF-{kappa}B translocation and ROS production in synoviocytes

    SciTech Connect (OSTI)

    Hu, Fen; Yang, Shuang; Zhao, Dan; Zhu, Shuyan; Wang, Yuxiang; Li, Junying

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Moderate extracellular acidification regulates intracellular Ca{sup 2+} mobilization. Black-Right-Pointing-Pointer Moderate acidification activates NF-{kappa}B nuclear translocation in synoviocytes. Black-Right-Pointing-Pointer Moderate acidification depresses the ROS production induced by capsaicin. Black-Right-Pointing-Pointer Moderate acidification inhibits capsaicin-caused synoviocyte death. -- Abstract: We previously show the expression of transient receptor potential vanilloid 1 (TRPV1) in primary synoviocytes from collagen-induced arthritis (CIA) rats. Capsaicin and lowered extracellular pH from 7.4 to 5.5 induce cell death through TRPV1-mediated Ca{sup 2+} entry and reactive oxygen species (ROS) production. However, under the pathological condition in rheumatoid arthritis, the synovial fluid is acidified to a moderate level (about pH 6.8). In the present study, we examined the effects of pH 6.8 on the TRPV1-mediated cell death. Our finding is different or even opposite from what was observed at pH 5.5. We found that the moderate extracellular acidification (from pH 7.4 to 6.8) inhibited the capsaicin-induced Ca{sup 2+} entry through attenuating the activity of TRPV1. In the mean time, it triggered a phospholipse C (PLC)-related Ca{sup 2+} release from intracellular stores. The nuclear translocation of NF-{kappa}B was found at pH 6.8, and this also depends on PLC activation. Moreover, the capsaicin-evoked massive ROS production and cell death were depressed at pH 6.8, both of which are dependent on the activation of PLC and NF-{kappa}B. Taken together, these results suggested that the moderate extracellular acidification inhibited the capsaicin-induced synoviocyte death through regulating Ca{sup 2+} mobilization, activating NF-{kappa}B nuclear translocation and depressing ROS production.

  1. ROS and NF-{kappa}B are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes

    SciTech Connect (OSTI)

    Ye Shefang Wu Yihui; Hou Zhenqing; Zhang Qiqing

    2009-02-06

    Carbon nanotubes (CNTs) have potential applications in biosensors, tissue engineering, and biomedical devices because of their unique physico-chemical, electronic and mechanical properties. However, there is limited literature data available concerning the biological properties and toxicity of CNTs. This study aimed to assess the toxicity exhibited by multi-walled CNTs (MWCNTs) and to elucidate possible molecular mechanisms underlying the biological effects of MWCNTs in A549 cells. Exposing A549 cells to MWCNTs led to cell death, changes in cell size and complexity, reactive oxygen species (ROS) production, interleukin-8 (IL-8) gene expression and nuclear factor (NF)-{kappa}B activation. Treatment of A549 cells with antioxidants prior to adding MWCNTs decreased ROS production and abrogated expression of IL-8 mRNA. Pretreatment of A549 cells with NF-{kappa}B inhibitors suppressed MWCNTs-induced IL-8 mRNA expression. These results indicate that MWCNTs are able to induce expression of IL-8 in A549 cells, at least in part, mediated by oxidative stress and NF-{kappa}B activation.

  2. Broken Valence Chiral Symmetry and Chiral Polarization of Dirac Spectrum in N$_f$=12 QCD at Small Quark Mass

    E-Print Network [OSTI]

    Andrei Alexandru; Ivan Horváth

    2014-12-04

    The validity of recently proposed equivalence between valence spontaneous chiral symmetry breaking (vSChSB) and chiral polarization of low energy Dirac spectrum (ChP) in SU(3) gauge theory, is examined for the case of twelve mass-degenerate fundamental quark flavors. We find that the vSChSB-ChP correspondence holds for regularized systems studied. Moreover, our results suggest that vSChSB occurs in two qualitatively different circumstances: there is a quark mass $m_c$ such that for $m > m_c$ the mode condensing Dirac spectrum exhibits standard monotonically increasing density, while for $m_{ch} < m < m_c$ the peak around zero separates from the bulk of the spectrum, with density showing a pronounced depletion at intermediate scales. Valence chiral symmetry restoration may occur at yet smaller masses $m < m_{ch}$, but this has not yet been seen by overlap valence probe, leaving the $m_{ch}=0$ possibility open. The latter option could place massless N$_f$=12 theory outside of conformal window. Anomalous behavior of overlap Dirac spectrum for $m_{ch} < m < m_c$ is qualitatively similar to one observed previously in zero and few-flavor theories as an effect of thermal agitation.

  3. Involvement of a chromatin modifier in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell injury: Probably an indirect action via the regulation of NF?B/FasL circuitry

    SciTech Connect (OSTI)

    Chen, Shiwei [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China)] [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China); Dong, Yushu [Department of Neurosurgery, 463rd Hospital of PLA, Shenyang 110042 (China)] [Department of Neurosurgery, 463rd Hospital of PLA, Shenyang 110042 (China); Xu, Chun; Jiang, Liming; Chen, Yongjie; Jiang, Cheng [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China)] [Department of Urology, 174th Hospital of PLA, Fujian 361001 (China); Hou, Wugang, E-mail: gangwuhou@163.com [Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032 (China)] [Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032 (China); Li, Wei, E-mail: liweipepeyato@163.com [Department of Human Anatomy, Histology and Embryology, Fourth Military Medical University, Xi’an 710032 (China)] [Department of Human Anatomy, Histology and Embryology, Fourth Military Medical University, Xi’an 710032 (China)

    2013-11-01

    Highlights: •MTA1 expression is upregulated in SCs upon MEHP treatment. •Knockdown of MTA1 in SCs impairs the MEHP-induced NF?B signaling activation. •Knockdown of MTA1 inhibits recruitment of NF?B onto FasL promoter in MEHP-treated SCs. -- Abstract: The Fas/FasL signaling pathway, controlled by nuclear factor-?B (NF?B) at the transcriptional level, is critical for triggering germ cell apoptosis in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell (SC) injury, but the exact regulation mechanism remain unknown. Here, we discovered that expression level of Metastasis associated protein 1 (MTA1), a component of the Mi-2/nucleosome remodeling and deacetylase complex, was upregulated in SCs during the early recovery after MEHP exposure. This expression change was in line with the dynamic changes in germ cell apoptosis in response to MEHP treatment. Furthermore, a knockdown of MTA1 by RNAi in SCs was found to impair the MEHP-induced early activation of NF?B pathway and abolish the recruitment of NF?B onto FasL promoter, which consequently diminished the MEHP-triggered FasL induction. Considering that Fas/FasL is a well characterized apoptosis initiating signaling during SCs injury, our results point to a potential “switch on” effect of MTA1, which may govern the activation of NF?B/FasL cascade in MEHP-insulted SCs. Overall, the MTA1/NF?B/FasL circuit may serve as an important defensive/repairing mechanism to help to control the germ cell quality after SCs injury.

  4. Rosiglitazone attenuates NF-{kappa}B-dependent ICAM-1 and TNF-{alpha} production caused by homocysteine via inhibiting ERK{sub 1/2}/p38MAPK activation

    SciTech Connect (OSTI)

    Bai, Yong-Ping [Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China); Liu, Yu-Hui [Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006 (China); Chen, Jia [Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China); Song, Tao [Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410078 (China); You, Yu [Department of Digestive Medicine, Xiangya Second Hospital, Central South University, Changsha, Hunan 410078 (China); Tang, Zhen-Yan [Department of Urologic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China); Li, Yuan-Jian [Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410078 (China); Zhang, Guo-Gang [Department of Cardiovascular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078 (China)]. E-mail: xyzgg2006@sina.com

    2007-08-17

    Previous studies demonstrated an important interaction between nuclear factor-kappaB (NF-{kappa}B) activation and homocysteine (Hcy)-induced cytokines expression in endothelial cells and vascular smooth muscle cells. However, the underlying mechanism remains illusive. In this study, we investigated the effects of Hcy on NF-{kappa}B-mediated sICAM-1, TNF-{alpha} production and the possible involvement of ERK{sub 1/2}/p38MAPK pathway. The effects of rosiglitazone intervention were also examined. Our results show that Hcy increased the levels of sICAM-1 and TNF-{alpha} in cultured human umbilical vein endothelial cells (HUVECs) in a time- and concentration-dependent manner. This effect was significantly depressed by rosiglitazone and different inhibitors (PDTC, NF-{kappa}B inhibitor; PD98059, MEK inhibitor; SB203580, p38MAPK specific inhibitor; and staurosporine, PKC inhibitor). Next, we investigated the effect of Hcy on ERK{sub 1/2}/p38MAPK pathway and NF-{kappa}B activity in HUVECs. The results show that Hcy activated both ERK{sub 1/2}/p38MAPK pathway and NF-{kappa}B-DNA-binding activity. These effects were markedly inhibited by rosiglitazone as well as other inhibitors (SB203580, PD98059, and PDTC). Further, the pretreatment of staurosporine abrogated ERK{sub 1/2}/p38MAPK phosphorylation, suggesting that Hcy-induced ERK{sub 1/2}/p38MAPK activation is associated with PKC activity. Our results provide evidence that Hcy-induced NF-{kappa}B activation was mediated by activation of ERK{sub 1/2}/p38MAPK pathway involving PKC activity. Rosiglitazone reduces the NF-{kappa}B-mediated sICAM-1 and TNF-{alpha} production induced by Hcy via inhibition of ERK{sub 1/2}/p38MAPK pa0011thw.

  5. Anti-neuroinflammatory efficacy of the aldose reductase inhibitor FMHM via phospholipase C/protein kinase C-dependent NF-?B and MAPK pathways

    SciTech Connect (OSTI)

    Zeng, Ke-Wu [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Li, Jun [Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029 (China); Dong, Xin; Wang, Ying-Hong; Ma, Zhi-Zhong; Jiang, Yong; Jin, Hong-Wei [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Tu, Peng-Fei, E-mail: pengfeitu@vip.163.com [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029 (China)

    2013-11-15

    Aldose reductase (AR) has a key role in several inflammatory diseases: diabetes, cancer and cardiovascular diseases. Therefore, AR inhibition seems to be a useful strategy for anti-inflammation therapy. In the central nervous system (CNS), microglial over-activation is considered to be a central event in neuroinflammation. However, the effects of AR inhibition in CNS inflammation and its underlying mechanism of action remain unknown. In the present study, we found that FMHM (a naturally derived AR inhibitor from the roots of Polygala tricornis Gagnep.) showed potent anti-neuroinflammatory effects in vivo and in vitro by inhibiting microglial activation and expression of inflammatory mediators. Mechanistic studies showed that FMHM suppressed the activity of AR-dependent phospholipase C/protein kinase C signaling, which further resulted in downstream inactivation of the I?B kinase/I?B/nuclear factor-kappa B (NF-?B) inflammatory pathway. Therefore, AR inhibition-dependent NF-?B inactivation negatively regulated the transcription and expression of various inflammatory genes. AR inhibition by FMHM exerted neuroprotective effects in lipopolysaccharide-induced neuron–microglia co-cultures. These findings suggested that AR is a potential target for neuroinflammation inhibition and that FMHM could be an effective agent for treating or preventing neuroinflammatory diseases. - Highlights: • FMHM is a natural-derived aldose reductase (AR) inhibitor. • FMHM inhibits various neuroinflammatory mediator productions in vitro and in vivo. • FMHM inhibits neuroinflammation via aldose reductase/PLC/PKC-dependent NF-?B pathway. • FMHM inhibits neuroinflammation via aldose reductase/PLC/PKC-dependent MAPK pathway. • FMHM protects neurons against inflammatory injury in microglia-neuron co-cultures.

  6. Mangiferin exerts hepatoprotective activity against D-galactosamine induced acute toxicity and oxidative/nitrosative stress via Nrf2–NF?B pathways

    SciTech Connect (OSTI)

    Das, Joydeep; Ghosh, Jyotirmoy; Roy, Anandita; Sil, Parames C.

    2012-04-01

    Mangiferin, a xanthone glucoside, is well known to exhibit antioxidant, antiviral, antitumor, anti-inflammatory and gene-regulatory effects. In the present study, we isolated mangiferin from the bark of Mangifera indica and assessed its beneficial role in galactosamine (GAL) induced hepatic pathophysiology. GAL (400 mg/kg body weight) exposed hepatotoxic rats showed elevation in the activities of serum ALP, ALT, levels of triglycerides, total cholesterol, lipid-peroxidation and reduction in the levels of serum total proteins, albumin and cellular GSH. Besides, GAL exposure (5 mM) in hepatocytes induced apoptosis and necrosis, increased ROS and NO production. Signal transduction studies showed that GAL exposure significantly increased the nuclear translocation of NF?B and elevated iNOS protein expression. The same exposure also elevated TNF-?, IFN-?, IL-1?, IL-6, IL-12, IL-18 and decreased IL-10 mRNA expressions. Furthermore, GAL also decreased the protein expression of Nrf2, NADPH:quinine oxidoreductase-1, heme oxygenase-1 and GST?. However, mangiferin administration in GAL intoxicated rats or coincubation of hepatocytes with mangiferin significantly altered all these GAL-induced adverse effects. In conclusion, the hepatoprotective role of mangiferin was due to induction of antioxidant defense via the Nrf2 pathway and reduction of inflammation via NF?B inhibition. Highlights: ?Galactosamine induces hepatocytes death via oxidative and nitrosative stress. ?Mangiferin exerts hepatoprotective effect/antioxidant defense via Nrf2 pathway. ?Mangiferin exerts anti-inflammatory responses by inhibiting NF-?B. ?Mangiferin suppresses galactosamine-induced repression of IL-10 mRNA.

  7. Hadron-Hadron Interactions from $N_f=2+1+1$ Lattice QCD: isospin-2 $\\pi\\pi$ scattering length

    E-Print Network [OSTI]

    Helmes, C; Knippschild, B; Liu, C; Liu, J; Liu, L; Urbach, C; Ueding, M; Wang, Z; Werner, M

    2015-01-01

    We present results for the $I=2$ $\\pi\\pi$ scattering length using $N_f=2+1+1$ twisted mass lattice QCD for three values of the lattice spacing and a range of pion mass values. Due to the use of Laplacian Heaviside smearing our statistical errors are reduced compared to previous lattice studies. A detailed investigation of systematic effects such as discretisation effects, volume effects, and pollution of excited and thermal states is performed. After extrapolation to the physical point using chiral perturbation theory at NLO we obtain $M_\\pi a_0=-0.0442(2)_\\mathrm{stat}(^{+4}_{-0})_\\mathrm{sys}$.

  8. NF_Eder_2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on771/6/14 Contact: Janet Lambert4 FLCNETL-ORD4NEVIS-NEWS MEDIAmail3

  9. TGF-{beta}1 increases invasiveness of SW1990 cells through Rac1/ROS/NF-{kappa}B/IL-6/MMP-2

    SciTech Connect (OSTI)

    Binker, Marcelo G.; CBRHC Research Center, Buenos Aires ; Binker-Cosen, Andres A.; Gaisano, Herbert Y.; Cosen, Rodica H. de; Cosen-Binker, Laura I.

    2011-02-04

    Research highlights: {yields} Rac1 mediates TGF-{beta}1-induced SW1990 invasion through MMP-2 secretion and activation. {yields} NADPH-generated ROS act downstream of Rac1 in TGF-{beta}1-challenged SW1990 cells. {yields} TGF-{beta}1-stimulated ROS activate NF-{kappa}B in SW1990 cells. {yields} NF{kappa}B-induced IL-6 release is required for secretion and activation of MMP-2 in SW1990 cells. -- Abstract: Human pancreatic cancer invasion and metastasis have been found to correlate with increased levels of active matrix metalloproteinase 2 (MMP-2). The multifunctional cytokine transforming growth factor beta 1 (TGF-{beta}1) has been shown to increase both secretion of MMP-2 and invasion by several pancreatic cancer cell types. In the present study, we investigated the signaling pathway involved in TGF-{beta}1-promoted MMP-2 secretion and invasion by human pancreatic cancer cells SW1990. Using specific inhibitors, we found that stimulation of these tumor cells with TGF-{beta}1 induced secretion and activation of the collagenase MMP-2, which was required for TGF-{beta}1-stimulated invasion. Our results also indicate that signaling events involved in TGF-{beta}1-enhanced SW1990 invasiveness comprehend activation of Rac1 followed by generation of reactive oxygen species through nicotinamide adenine dinucleotide phosphate-oxidase, activation of nuclear factor-kappa beta, release of interleukin-6, and secretion and activation of MMP-2.

  10. 4-Hydroxyestradiol induces oxidative stress and apoptosis in human mammary epithelial cells: possible protection by NF-{kappa}B and ERK/MAPK

    SciTech Connect (OSTI)

    Chen Zhihua [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of); Na, Hye-Kyung [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of); Hurh, Yeon-Jin [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of); Surh, Young-Joon [National Research Laboratory of Molecular Carcinogenesis and Chemoprevention, College of Pharmacy, Seoul National University, Shinlim-dong, Kwanak-ku, Seoul 151-742 (Korea, Republic of)]. E-mail: surh@plaza.snu.ac.kr

    2005-10-01

    Catechol estrogens, the hydroxylated metabolites of 17{beta}-estradiol (E{sub 2}), have been considered to be implicated in estrogen-induced carcinogenesis. 4-Hydroxyestradiol (4-OHE{sub 2}), an oxidized metabolite of E{sub 2} formed preferentially by cytochrome P450 1B1, reacts with DNA to form depurinating adducts thereby exerting genotoxicity and carcinogenicity. 4-OHE{sub 2} undergoes 2-electron oxidation to quinone via semiquinone, and during this process, reactive oxygen species (ROS) can be generated to cause DNA damage and cell death. In the present study, 4-OHE{sub 2} was found to elicit cytotoxicity in cultured human mammary epithelial (MCF-10A) cells, which was blocked by the antioxidant trolox. MCF-10A cells treated with 4-OHE{sub 2} exhibited increased intracellular ROS accumulation and 8-oxo-7,8-dihydroxy-2'-deoxyguanosine formation, and underwent apoptosis as determined by poly(ADP-ribose)polymerase cleavage and disruption of mitochondrial transmembrane potential. The redox-sensitive transcription factor nuclear factor {kappa}B (NF-{kappa}B) was transiently activated by 4-OHE{sub 2} treatment. Cotreatment of MCF-10A cells with the NF-{kappa}B inhibitor, L-1-tosylamido-2-phenylethyl chloromethyl ketone, exacerbated 4-OHE{sub 2}-induced cell death. 4-OHE{sub 2} also caused transient activation of extracellular signal-regulated protein kinases (ERK) involved in transmitting cell survival or death signals. A pharmacological inhibitor of ERK aggravated the 4-OHE{sub 2}-induced cytotoxicity, supporting the pivotal role of ERK in protecting against catechol estrogen-induced oxidative cell death.

  11. Hypo-responsiveness of interleukin-8 production in human embryonic epithelial intestine 407 cells independent of NF-{kappa}B pathway: New lessons from endotoxin and ribotoxic deoxynivalenol

    SciTech Connect (OSTI)

    Moon, Yuseok [Department of Microbiology and Immunology, Medical Research Institute, Pusan National University School of Medicine, Busan, 602-739 (Korea, Republic of)], E-mail: moon@pusan.ac.kr; Yang, Hyun; Park, Seung-Hwan [Department of Microbiology and Immunology, Medical Research Institute, Pusan National University School of Medicine, Busan, 602-739 (Korea, Republic of)

    2008-08-15

    Mucosal epithelium senses external toxic insults and transmits the danger signals into the epithelial cells in order to activate a broad range of inflammatory responses. However, pre-exposure to the commensal endotoxins can induce inflammatory tolerance and maintain the homeostasis without excessive immune responses. We recently reported that ribotoxin deoxynivalenol (DON) and its derivatives elicited the pro-inflammatory response as the mucosal insults in human epithelial cells. Taking the knowledge into consideration, we tested the hypothesis that endotoxin pre-exposure can attenuate ribotoxin-induced epithelial interleukin-8 (IL-8) production via a tolerance mechanism. Pre-exposure to endotoxin repressed IL-8 release and its gene expression. However, inflammatory tolerance was not mediated by the attenuated NF-{kappa}B activation which has been generally recognized as the major mediator of LPS-mediated toll-like receptor (TLR) signaling pathway. Instead, pre-exposure to endotoxin was observed to trigger the delayed induction of peroxisome proliferator-activated receptor gamma (PPAR-{gamma}) which contributed to the diminished IL-8 production in the human epithelial cells. Moreover, endogenous PPAR-{gamma} agonist suppressed toxicant-mediated interleukin-8 production and IL-8 mRNA stability. Taken together, endotoxin induced hypo-production of pro-inflammatory cytokine IL-8 in the human epithelial cells, which was associated with the delayed activation of PPAR-{gamma} expression by pre-existing endotoxin.

  12. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  13. Activation of ROS/NF-{kappa}B and Ca{sup 2+}/CaM kinase II are necessary for VCAM-1 induction in IL-1{beta}-treated human tracheal smooth muscle cells

    SciTech Connect (OSTI)

    Luo, S.-F. [Department of Internal Medicine, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (China); Chang, C.-C.; Lee, I-T. [Department of Physiology and Pharmacology, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (China); Lee, C.-W. [Department of Nursing, Division of Basic Medical Sciences, Chang Gung Institute of Technology, Chia-Yi, Taiwan (China); Lin, W.-N. [Department of Physiology and Pharmacology, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (China); Lin, C.-C. [Department of Anesthetics, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (China); Yang, C.-M. [Department of Physiology and Pharmacology, Chang Gung University, Kwei-San, Tao-Yuan, Taiwan (China)], E-mail: chuenmao@mail.cgu.edu.tw

    2009-05-15

    Histone acetylation regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs) plays a critical role in the expression of inflammatory genes, such as vascular cell adhesion molecule-1 (VCAM-1). Oxidative processes have been shown to induce VCAM-1 expression. Here, we investigated the mechanisms underlying IL-1{beta}-induced VCAM-1 expression in human tracheal smooth muscle cells (HTSMCs). Our results showed that IL-1{beta} enhanced HTSMCs-monocyte adhesion through up-regulation of VCAM-1, which was inhibited by pretreatment with selective inhibitors of PKC{alpha} (Goe6976), c-Src (PP1), NADPH oxidase [diphenylene iodonium (DPI) and apocynin (APO)], intracellular calcium chelator (BAPTA/AM), PI-PLC (U73122), CaM (calmidazolium chloride), CaM kinase II (KN62), p300 (garcinol), NF-{kappa}B (Bay11-7082), HDAC (trichostatin A), and ROS scavenger [N-acetyl-L-cysteine (NAC)] or transfection with siRNAs of MyD88, PKC{alpha}, Src, p47{sup phox}, p300, and HDAC4. Moreover, IL-1{beta} stimulated NF-{kappa}B and CaMKII phosphorylation through MyD88-dependent PI-PLC/PKC{alpha}/c-Src/ROS and PI-PLC/Ca{sup 2+}/CaM pathways, respectively. Activation of NF-{kappa}B and CaMKII may eventually lead to the acetylation of histone residues and phosphorylation of histone deacetylases. These findings suggested that IL-1{beta} induced VCAM-1 expression via these multiple signaling pathways in HTSMCs. Blockade of these pathways may reduce monocyte adhesion via VCAM-1 suppression and attenuation of the inflammatory responses in airway diseases.

  14. The REMOTE SENSlNf

    Office of Legacy Management (LM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal Gas &SCE-SessionsSouth DakotaRobbins and700, 1. .&. ' , c 1afr -.:2yL: a,;. -:-1 / .'

  15. Propofol pretreatment attenuates LPS-induced granulocyte-macrophage colony-stimulating factor production in cultured hepatocytes by suppressing MAPK/ERK activity and NF-{kappa}B translocation

    SciTech Connect (OSTI)

    Jawan, Bruno [Department of Anesthesiology and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Kao, Y.-H. [Department of Anesthesiology and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung 804, Taiwan (China); Goto, Shigeru [Department of Surgery and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Department of Surgery, Iwao Hospital, 3059-1 Kawakami, Yufuin, Oita 879-5102 (Japan); Pan, M.-C. [Department of Anesthesiology and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Lin, Y.-C.; Hsu, L.-W.; Nakano, Toshiaki; Lai, C.-Y.; Sun, C.-K.; Cheng, Y.-F. [Department of Surgery and Liver Transplantation Program, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Chang Gung University College of Medicine, 123 Ta-Pei Road, Niao-Sung Hsiang, Kaohsiung Hsien 833, Taiwan (China); Tai, M.-H. [Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung 804, Taiwan (China); Department of Medical Research and Education, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Road, Kaohsiung 813, Taiwan (China)] (and others)

    2008-06-15

    Propofol (PPF), a widely used intravenous anesthetic for induction and maintenance of anesthesia during surgeries, was found to possess suppressive effect on host immunity. This study aimed at investigating whether PPF plays a modulatory role in the lipopolysaccharide (LPS)-induced inflammatory cytokine expression in a cell line of rat hepatocytes. Morphological observation and viability assay showed that PPF exhibits no cytotoxicity at concentrations up to 300 {mu}M after 48 h incubation. Pretreatment with 100 {mu}M PPF for 24 h prior to LPS stimulation was performed to investigate the modulatory effect on LPS-induced inflammatory gene production. The results of semi-quantitative RT-PCR demonstrated that PPF pretreatment significantly suppressed the LPS-induced toll-like receptor (TLR)-4, CD14, tumor necrosis factor (TNF)-{alpha}, and granulocyte-macrophage colony-stimulating factor (GM-CSF) gene expression. Western blotting analysis showed that PPF pretreatment potentiated the LPS-induced TLR-4 downregulation. Flow cytometrical analysis revealed that PPF pretreatment showed no modulatory effect on the LPS-upregulated CD14 expression on hepatocytes. In addition, PPF pretreatment attenuated the phosphorylation of mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and I{kappa}B{alpha}, as well as the nuclear translocation of NF-{kappa}B primed by LPS. Moreover, addition of PD98059, a MAPK kinase inhibitor, significantly suppressed the LPS-induced NF-{kappa}B nuclear translocation and GM-CSF production, suggesting that the PPF-attenuated GM-CSF production in hepatocytes may be attributed to its suppressive effect on MAPK/ERK signaling pathway. In conclusion, PPF as an anesthetic may clinically benefit those patients who are vulnerable to sepsis by alleviating sepsis-related inflammatory response in livers.

  16. Mercury Chamber NF-IDS Meeting

    E-Print Network [OSTI]

    McDonald, Kirk

    windows (upstream and downstream) ­ Shell cooling? #12;4 Managed by UT-Battelle for the U.S. Department Upstream View Downstream View #12;6 Managed by UT-Battelle for the U.S. Department of Energy Mercury Isometrics Downstream Beam Window Drainage Notch Overflow & Maintenance Drains Nozzle, Beam Pipe, Vents

  17. Solid Target Studies for NF Rob Edgecock

    E-Print Network [OSTI]

    McDonald, Kirk

    . Proposed lubricant (coating): WS2 Demonstrated to work to ~1300oC in vacuum. #12;Target Change #12;Target Calculations and Optimisation. John 4. Chain Design Optimisation ­ roller chain or alternative. David Consult

  18. NF Energy Saving Corp | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QAsource History ViewMayo, Maryland: EnergyInformationOliver,Minnesota:EnergyNARI JumpNEXTGen Syndicate LLC

  19. IDS NF Target StudiesIDS-NF Target Studies H. Kirk (BNL)( )

    E-Print Network [OSTI]

    McDonald, Kirk

    20 40 60 80 100 Proton Kinetic Energy, GeV #12;Meson Production EfficiencyMeson Production Efficiency

  20. NF kappa B expression and matrix metalloproteinase activity in hypertension

    E-Print Network [OSTI]

    Wu, Kwan-I

    2009-01-01

    14% (Fig. 3-1), and in hypothalamus region of brain by 22% (?B expression level in hypothalamus region of the brain. Forp65 subunit in the hypothalamus. N=4 rats for each group. *p

  1. N_f=2+1 flavour equation of state

    E-Print Network [OSTI]

    Szabolcs Borsanyi; Gergely Endrodi; Zoltan Fodor; Antal Jakovac; Sandor D. Katz; Stefan Krieg; Claudia Ratti; Kalman K. Szabo

    2010-11-18

    We conclude our investigation on the QCD equation of state (EoS) with 2+1 staggered flavors and one-link stout improvement. We extend our previous study [JHEP 0601:089 (2006)] by choosing even finer lattices. These new results [for details see arXiv:1007.2580] support our earlier findings. Lattices with N_t=6,8 and 10 are used, and the continuum limit is approached by checking the results at N_t=12. A Symanzik improved gauge and a stout-link improved staggered fermion action is taken; the light and strange quark masses are set to their physical values. Various observables are calculated in the temperature (T) interval of 100 to 1000~MeV. We compare our data to the equation of state obtained by the "hotQCD" collaboration.

  2. NF 3 , the greenhouse gas missing from Kyoto

    E-Print Network [OSTI]

    Prather, Michael J; Hsu, Juno

    2008-01-01

    of the world’s largest coal-fired power plants. If released,the world’s largest coal-fired power stations (e.g. , 25 toO b CH 4b CO 2b 3600 MW Coal-Fired Plants Scherer (Georgia,

  3. ARM - VAP Product - aerich1nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach HomeA Better AnodeThe Influence ofrlprofasr1ferrProductsabrfcabrfcprecip Documentation abrfc :

  4. ARM - VAP Product - aerich2nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach HomeA Better AnodeThe Influence ofrlprofasr1ferrProductsabrfcabrfcprecip Documentation abrfc

  5. CMRR-NF Supplemental EIS Scoping Comments | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefield Municipal Gas &SCE-SessionsSouthReporteeo | National Nucleara minNuclear SecurityREQUIREMENTS

  6. Gradient flow and IR fixed point in SU(2) with Nf=8 flavors

    E-Print Network [OSTI]

    Viljami Leino; Tuomas Karavirta; Jarno Rantaharju; Teemu Rantalaiho; Kari Rummukainen; Joni M. Suorsa; Kimmo Tuominen

    2015-11-11

    We study the running of the coupling in SU(2) gauge theory with 8 massless fundamental representation fermion flavours, using the gradient flow method with the Schr\\"odinger functional boundary conditions. Gradient flow allows us to measure robust continuum limit for the step scaling function. The results show a clear indication of infrared fixed point consistent with perturbation theory.

  7. DEPOSITED POWER STUDIES FOR THE MC/NF TARGET STATION. Nicholas Souchlas (PBL)

    E-Print Network [OSTI]

    McDonald, Kirk

    . SHIELDING MATERIAL TO PROTECT SC COILS. SHIELDING VESSELS TO CONTAIN SHIELDING MATERIAL. SC SOLENOIDS CRYOGENIC COOLING COMPONENTS. LIQUID TARGET COLLECTING TANK (+ BEAM DUMP) AND REMOVAL CONFIGURATION. 2 15 55 #12;SHIELDING MATERIAL OPTIMIZATION (TYPE/QUANTITY) MINIMIZE DEMAND ON SC CRYOGENIC OPERATIONS. AVOID

  8. Effects of water chemistry on NF/RO membrane structure and performance

    E-Print Network [OSTI]

    Mo, Yibing

    2013-01-01

    1,4-dioxane…. ……………… 3.4. Water chemistry: Concentration,for EG………………………………………. 4.3.1. Effect of water chemistryon membrane water permeability … 4.3.1.1. Effect of ionic

  9. Materials Data on H11NF8 (SG:14) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  10. Materials Data on H7NF4 (SG:161) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. Materials Data on H8NF5 (SG:88) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. Materials Data on TiH12(NF2)4 (SG:56) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  13. Materials Data on H4NF (SG:186) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  14. Thermal NF3 fluorination/oxidation of cobalt, yttrium, zirconium, and selected lanthanide oxides

    SciTech Connect (OSTI)

    Scheele, Randall D.; McNamara, Bruce K.; Casella, Andrew M.; Kozelisky, Anne E.; Neiner, Doinita

    2013-02-01

    This paper presents results of our continuing investigation on the use of nitrogen trifluoride as a fluorination or fluorination/oxidation agent for separating valuable constituents from used nuclear fuels by exploiting the different volatilities of the constituent fission product and actinide fluorides. This article focuses on fission products that do not have volatile fluorides or oxyfluorides at expected operations temperatures. Our thermodynamic calculations show that nitrogen trifluoride has the potential to completely fluorinate fission product oxides to their fluorides. Simultaneous thermogravimetric and differential thermal analyses show that the oxides of cobalt, zirconium, and the lanthanides are fluorinated but do not form volatile fluorides when treated with nitrogen trifluoride at temperatures up to 550°C. Our studies of gadolinium-doped commercial nuclear fuel indicate that nitrogen trifluoride can extract uranium from the non-volatile gadolinium.

  15. Acknowledgements. This work was partially supported by NSF CAREER IIS-0845282, ARO YIP W911NF-11-1-0090 and DARPA Mind's Eye W911NF-10-2-0062.

    E-Print Network [OSTI]

    Krovi, Venkat

    in the final segmentation based on various post hoc feature criteria, such as motion, object-ness or human-ness: - Unsupervised: Motion. - Supervised, Category-Independent: Object-ness. - Supervised: Human-ness, Car-ness, etc and Flattening) UES on Human-ness (Selection and Flattening) UES on Car-ness (Selection a

  16. DETEW!INATION OF THE NEUTRON MAGNETIC FlONENT G.L. Greene and N.F. Ramsey

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would like submit theCovalent Bonding Low-Cost Ground8 Gas

  17. XIAP and cIAP1 amplifications induce Beclin 1-dependent autophagy through NF?B activation

    E-Print Network [OSTI]

    Lin, Fang; Ghislat, Ghita; Luo, Shouqing; Renna, Maurizio; Siddiqi, Farah; Rubinsztein, David C.

    2015-02-10

    and on the cell or tissue context. Furthermore, it is possible that the expression of certain proteins associated with cancer riskmayaffect both autophagy and apoptosis and the pro- ducts of this interplay may be relevant to the disease. Inhibitors of apoptosis...

  18. JNK-mediated turnover and stabilization of the transcription factor p45/NF-E2 during differentiation

    E-Print Network [OSTI]

    Tsai, Ming-Daw

    of transcription factors is essential for correct programming of cell proliferation and differentiation. We have and nuclear hormones all play important roles in the maturation of erythroid cells. For exam- ple, it is known that signaling from the erythropoietin (Epo) receptor activates several pathways to promote cell proliferation

  19. Functions of receptor activator of NF-?B ligand (RANKL) and its receptors, RANK and OPG, are evolutionarily conserved 

    E-Print Network [OSTI]

    Sutton, Kate Maurice

    2014-11-28

    The tumour necrosis factor (TNF) superfamily is a group of cytokines that orchestrate a variety of functions, both in the development of the architecture of immune organs and of the immune response. The mammalian TNF ...

  20. Stepwise contraction of the nf Rydberg shells in the 3d photoionization of multiply-charged xenon ions

    E-Print Network [OSTI]

    Schippers, S; Buhr, T; Hellhund, J; Holste, K; Kilcoyne, A L D; Klumpp, S; Martins, M; Müller, A; Ricz, S; Fritzsche, S

    2014-01-01

    Triple photoionization of Xe3+, Xe4+ and Xe5+ ions has been studied in the energy range 670-750 eV, including the 3d ionization threshold. The photon-ion merged-beam technique was used at a synchrotron light source to measure the absolute photoionization cross sections. These cross sections exhibit a progressively larger number of sharp resonances as the ion charge state is increased. This clearly visualizes the re-ordering of the $\\epsilon$f continuum into a regular series of (bound) Rydberg orbitals as the ionic core becomes more attractive. The energies and strengths of the resonances are extracted from the experimental data and are further analyzed by relativistic atomic-structure calculations.

  1. QCD thermodynamics with $N_f=2+1$ near the continuum limit at realistic quark masses

    E-Print Network [OSTI]

    Takashi Umeda

    2006-10-03

    We report on our study of QCD thermodynamics with 2+1 flavors of dynamical quarks. In this proceeding we present several thermodynamic quantities and our recent calculation of the critical temperature. In order to investigate the thermodynamic properties of QCD near the continuum limit we adopt improved staggered (p4) quarks coupled with tree-level Symanzik improved glue on $N_t=4$ and 6 lattices. The simulations are performed with a physical value of the strange quark mass and light quark masses which are in the range of $m_q/m_s=0.05-0.4$. The lightest quark mass corresponds to a pion mass of about 150 MeV.

  2. An Epigenetic Switch Involving NF-kB, Lin28, Let-7 MicroRNA, and IL6 Links

    E-Print Network [OSTI]

    proinflammatory cytokines and chemokines, seems to be a key molecular link between inflammation and oncogenesis

  3. Modulation of host NF-[k??a?p?p?a?]B pathway by the Toxoplasma gondii secreted factor, GRA15

    E-Print Network [OSTI]

    Lu, Diana, Ph. D. Massachusetts Institute of Technology

    2013-01-01

    The apicomplexan protozoan Toxoplasma gondii is an obligate intracellular pathogen that infects all warm blooded animals, including nearly thirty percent of the human population worldwide. Toxoplasma's success as a parasite ...

  4. ineralization of acid and sweet whey. There would be about 150 000200 000 m2 of NF

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    properties [13]. In crossflow filtration of whey using organic membranes, the most important fouling to fouling. Some studies have demonstrated that the hydrodynamic operating conditions of mem- brane crossflow

  5. Enhancement of CD8+ T-cell memory by removal of a vaccinia virus NF-?B inhibitor

    E-Print Network [OSTI]

    Ren, Hongwei; Ferguson, Brian J.; de Motes, Carlos Maluquer; Sumner, Rebecca P.; Harman, Laura; Smith, Geoffrey L.

    2014-11-07

    % paraformaldehyde (Sigma-Aldrich, St Louis, MO). Flow cytometry was performed with a BD LSR Fortessa (BD Biosciences), and data were analysed with FLOWJO software (Tree Star Inc., Ashland, OR). LIVE/DEAD? Fixable Aqua Dead Cell Stain Kit (Life Technologies, Paisley... , UK) was used to exclude non-via- ble cells from analysis. Flow cytometric gating strategies are shown in Supplementary Figure S3. DimerX assay to detect VACV specific CD8+ T cells Recombinant soluble dimeric mouse H-2Kb:Ig fusion pro- teins were...

  6. MODULATION OF THE NF-KAPPA B SIGNALING PATHWAY BY THE BACTERIAL TYPE III SECRETION SYSTEM EFFECTORS

    E-Print Network [OSTI]

    Gao, Xiaofei

    2012-08-31

    G PAMPs: Pathogen associated molecular patterns PRRs: Pattern recognition receptors PI: Phosphoinositide RING: Really Interesting New Gene RPS3: Ribosomal protein subunit 3 Stx: Shiga toxins TAK1: Transforming growth factor-activated protein....coli) is one of the most studied microorganism. It has been used as a model organism for a variety of biological purposes. Recent studies showed that E. coli is an extremely versatile organism with high genome plasticity (Hacker et al., 1997). Studies in E...

  7. 1992 CBECS C & E

    U.S. Energy Information Administration (EIA) Indexed Site

    of District Heat by End Use, 1989 District Heat Consumption (trillion Btu) Space Water a Total Heating Heating Other RSE Building Row Characteristics Factor 1.0 NF NF NF RSE...

  8. 1992 CBECS C & E

    U.S. Energy Information Administration (EIA) Indexed Site

    0. Consumption of Fuel Oil by End Use, 1989 Fuel Oil Consumption (trillion Btu) Space Water a Total Heating Heating Other RSE Building Row Characteristics Factor 1.0 NF NF NF RSE...

  9. Microstructural evolution in NF616 (P92) and Fee9Cre0.1C-model alloy under heavy ion irradiation

    E-Print Network [OSTI]

    Motta, Arthur T.

    .V. All rights reserved. 1. Introduction High chromium (9e12 wt.%) ferritic-martensitic (FeM) steels]. Normalized and tempered 9e12 wt.% FeM steels typically exhibit the tempered martensite structure reactor Heavy ion irradiation In situ technique Transmission electron microscopy Ferritic-martensitic

  10. TWIST1 associates with NF-kappaB subunit RELA via carboxyl-terminal WR domain to promote cell autonomous invasion through IL8 production

    E-Print Network [OSTI]

    2012-01-01

    in (A). Scale bar, 160 ?m. (D) Gelatin zymography assays ofin ECM degradation. Using gelatin zymography, we found thatmanufac- turer’s guidelines. Gelatin zymography Conditioned

  11. TNF{alpha} induced FOXP3-NF{kappa}B interaction dampens the tumor suppressor role of FOXP3 in gastric cancer cells

    SciTech Connect (OSTI)

    Hao, Qiang; Li, Weina; Zhang, Cun; Qin, Xin; Xue, Xiaochang; Li, Meng; Shu, Zhen; Xu, Tianjiao; Xu, Yujin; Wang, Weihua [The State Key Laboratory of Cancer Biology, School of Pharmacy, Department of Biopharmaceutics, Fourth Military Medical University, Xi'an 710032 (China)] [The State Key Laboratory of Cancer Biology, School of Pharmacy, Department of Biopharmaceutics, Fourth Military Medical University, Xi'an 710032 (China); Zhang, Wei, E-mail: Zhangw90@fmmu.edu.cn [The State Key Laboratory of Cancer Biology, School of Pharmacy, Department of Biopharmaceutics, Fourth Military Medical University, Xi'an 710032 (China)] [The State Key Laboratory of Cancer Biology, School of Pharmacy, Department of Biopharmaceutics, Fourth Military Medical University, Xi'an 710032 (China); Zhang, Yingqi, E-mail: Zhangyqh@fmmu.edu.cn [The State Key Laboratory of Cancer Biology, School of Pharmacy, Department of Biopharmaceutics, Fourth Military Medical University, Xi'an 710032 (China)] [The State Key Laboratory of Cancer Biology, School of Pharmacy, Department of Biopharmaceutics, Fourth Military Medical University, Xi'an 710032 (China)

    2013-01-04

    Highlights: Black-Right-Pointing-Pointer FOXP3 inhibition of cell proliferation is p21-dependent under basal conditions. Black-Right-Pointing-Pointer Inflammation induced by TNF{alpha} inhibits the tumor suppressor role of FOXP3. Black-Right-Pointing-Pointer Interaction between p65 and FOXP3 inhibits p21 transcription activation. -- Abstract: Controversial roles of FOXP3 in different cancers have been reported previously, while its role in gastric cancer is largely unknown. Here we found that FOXP3 is unexpectedly upregulated in some gastric cancer cells. To test whether increased FOXP3 remains the tumor suppressor role in gastric cancer as seen in other cancers, we test its function in cell proliferation both at basal and TNF{alpha} mimicked inflammatory condition. Compared with the proliferation inhibitory role observed in basal condition, FOXP3 is insufficient to inhibit the cell proliferation under TNF{alpha} treatment. Molecularly, we found that TNF{alpha} induced an interaction between FOXP3 and p65, which in turn drive the FOXP3 away from the promoter of the well known target p21. Our data here suggest that although FOXP3 is upregulated in gastric cancer, its tumor suppressor role has been dampened due to the inflammation environment.

  12. GaAs 0.5 dB NF dual-loop negative-feedback broadband low-noise amplifier IC

    E-Print Network [OSTI]

    Serdijn, Wouter A.

    ) designed for the square kilometre array is presented. Effects of transformer non-idealities on LNA is obtained by increasing the collecting area to approxi- mately one square kilometre. This square kilometre array (SKA) [1] employs approximately 100 million antennas that are connected in a phased array, forming

  13. Leading-order hadronic contribution to the anomalous magnetic moment of the muon from N_f=2+1+1 twisted mass fermions

    SciTech Connect (OSTI)

    Burger, Florian [Humboldt U. Berlin; Feng, Xu [KEK; Hotzel, Grit [Humboldt U. Berlin; Jansen, Karl [DESY; Petschlies, Marcus [The Cyprus Institute; Renner, Dru B. [JLAB

    2013-11-01

    We present results for the leading order QCD correction to the anomalous magnetic moment of the muon including the first two generations of quarks as dynamical degrees of freedom. Several light quark masses are examined in order to yield a controlled extrapolation to the physical pion mass. We analyse ensembles for three different lattice spacings and several volumes in order to investigate lattice artefacts and finite-size effects, respectively. We also provide preliminary results for this quantity for two flavours of mass-degenerate quarks at the physical value of the pion mass.

  14. Distinct NF-kB activation pathways engaged by T-cell receptor and co-receptor CD28 on T-cells

    E-Print Network [OSTI]

    Thaker, Youg Raj; Rudd, Christopher E

    2015-03-02

    at the pro-T3 stage [16]. In addition to this, Jurkat T-cell mutant lines lacking either SLP-76 or LAT expression showed a vital requirement of both molecules in mediating TCR-induced PLC?1 phosphorylation, extracellular signal-regulated kinase (ERK...

  15. Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK/JNK-dependent activation of NF-jB in THP-1 cells

    E-Print Network [OSTI]

    Lee, Won-Ha

    Stimulation of Fas (CD95) induces production of pro-inflammatory mediators through ERK is mediated by mitogen activated protein kinases (MAPKs) including extracellular-signal-regulated kinase (ERK inducing molecule through interaction with its counterpart and Fas-mediate events are mediated by ERK

  16. Regulation of cytochrome P450 3A4 gene expression through modulating pregnane X receptor transcriptional activity by NF-? aryl hydrocarbon receptor and xenobiotics 

    E-Print Network [OSTI]

    Gu, Xinsheng

    2009-05-15

    Cytochrome P450 3A4 (CYP3A4) is a key enzyme responsible for the metabolism of drugs and endogenous compounds in human liver and intestine. CYP3A4 gene expression is mainly regulated by Pregnane X receptor (PXR) which is ...

  17. The Low Energy Constants of $SU(2)$ Partially Quenched Chiral Perturbation Theory from $N_{f}=2+1$ Domain Wall QCD

    E-Print Network [OSTI]

    Boyle, P A; Garron, N; Jung, C; Jüttner, A; Kelly, C; Mawhinney, R D; McGlynn, G; Murphy, D J; Ohta, S; Portelli, A; Sachrajda, C T

    2015-01-01

    We have performed fits of the pseudoscalar masses and decay constants, from a variety of RBC-UKQCD domain wall fermion ensembles, to $SU(2)$ partially quenched chiral perturbation theory at next-to leading order (NLO) and next-to-next-to leading order (NNLO). We report values for 9 NLO and 8 linearly independent combinations of NNLO partially quenched low energy constants, which we compare to other lattice and phenomenological determinations. We discuss the size of successive terms in the chiral expansion and use our large set of low energy constants to make predictions for mass splittings due to QCD isospin breaking effects and the S-wave $\\pi \\pi$ scattering lengths. We conclude that, for the range of pseudoscalar masses explored in this work, $115~\\mathrm{MeV} \\lesssim m_{\\rm PS} \\lesssim 430~\\mathrm{MeV}$, the NNLO $SU(2)$ expansion is quite robust and can fit lattice data with percent-scale accuracy.

  18. A Smac-mimetic sensitizes prostrate cancer cells to TRAIL-induced apoptosis via modulating IAPs and NF-kappaB

    E-Print Network [OSTI]

    Dai, Yao; Liu, Meilan; Tang, Wenhua; Li, Yongming; Lian, Jiqin; Lawrence, Theodore S.; Xu, Liang

    2009-11-06

    Background: Although tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising agent for human cancer therapy, prostate cancer still remains resistant to TRAIL. Both X-linked inhibitor of apoptosis (XIAP) and nuclear factor...

  19. Structure and Biochemistry of IKappaB Zeta : : A Nuclear IKappaB Protein

    E-Print Network [OSTI]

    Zhu, Norman L.

    Bcl-3 is an antagonist of pSO/NF-?B-mediated inhibition. ,Bcl-3 is an antagonist of pSO/NF-?B-mediated inhibition. ,

  20. Plant polyphenols differentially modulate inflammatory responses of human keratinocytes by interfering with activation of transcription factors NF{kappa}B and AhR and EGFR-ERK pathway

    SciTech Connect (OSTI)

    Potapovich, Alla I. [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Biology Department, Belarus State University, Skorina Prosp. 10, Minsk 220050 (Belarus); Lulli, Daniela; Fidanza, Paolo [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Kostyuk, Vladimir A. [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Biology Department, Belarus State University, Skorina Prosp. 10, Minsk 220050 (Belarus); De Luca, Chiara; Pastore, Saveria [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy); Korkina, Liudmila G., E-mail: l.korkina@idi.it [Tissue Engineering and Skin Pathophysiology Laboratory, Dermatology Research Institute (IDI IRCCS), Via Monti di Creta 104, Rome 00167 (Italy)

    2011-09-01

    Molecular mechanisms underlying modulation of inflammatory responses in primary human keratinocytes by plant polyphenols (PPs), namely the glycosylated phenylpropanoid verbascoside, the stilbenoid resveratrol and its glycoside polydatin, and the flavonoid quercetin and its glycoside rutin were evaluated. As non-lethal stimuli, the prototypic ligand for epidermal growth factor receptor (EGFR) transforming growth factor alpha (TGFalpha), the combination of tumor necrosis factor (TNFalpha) and interferon (IFNgamma) (T/I), UVA + UVB irradiation, and bacterial lipopolysaccharide (LPS) were used. We demonstrated differential modulation of inflammatory responses in keratinocytes at signal transduction, gene transcription, and protein synthesis levels as a function of PP chemical structure, the pro-inflammatory trigger used, and PP interaction with intracellular detoxifying systems. The PPs remarkably inhibited constitutive, LPS- and T/I-induced but not TGFalpha-induced ERK phosphorylation. They also suppressed NFkappaB activation by LPS and T/I. Verbascoside and quercetin invariably impaired EGFR phosphorylation and UV-associated aryl hydrocarbon receptor (AhR)-mediated signaling, while rutin, polydatin and resveratrol did not affect EGFR phosphorylation and further activated AhR machinery in UV-exposed keratinocytes. In general, PPs down-regulated gene expression of pro-inflammatory cytokines/enzymes, except significant up-regulation of IL-8 observed under stimulation with TGFalpha. Both spontaneous and T/I-induced release of IL-8 and IP-10 was suppressed, although 50 {mu}M resveratrol and polydatin up-regulated IL-8. At this concentration, resveratrol activated both gene expression and de novo synthesis of IL-8 and AhR-mediated mechanisms were involved. We conclude that PPs differentially modulate the inflammatory response of human keratinocytes through distinct signal transduction pathways, including AhR and EGFR. - Graphical abstract: Display Omitted Highlights: > Effects of plant polyphenols on inflammatory responses in human keratinocytes. > Inflammatory stimuli used: TGFalpha, TNFalpha+IFNgamma, UVA+UVB, and LPS. > Inflammatory pathways connected with NFB, ERK1/2, EGFR, and AhR were investigated. > Plant polyphenols, flavonoids, stilbenoids, and phenylpropanoids, were studied. > Modulation of inflammation depends on phenolic core structure and glycosylation.

  1. NF-kappa B- and AP-1-mediated induction of human beta defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917: A novel effect of a probiotic bacterium

    E-Print Network [OSTI]

    2004-01-01

    and E. Isolauri. 2002. Probiotics: an overview of bene?cial17. Isolauri, E. 2001. Probiotics in human disease. Am. J.and S. Salminen. 2002. Probiotics: a role in the treatment

  2. Bestelmeyer, BT, GS Okin, MC Duniway, SR Archer, NF Sayre, JC Williamson, JE Herrick. 2014. Desertification, land use and the transformation of global drylands. Frontiers in Ecology and the

    E-Print Network [OSTI]

    Archer, Steven R.

    . Desertification, land use and the transformation of global drylands. Frontiers in Ecology and the Environment : In Press Desertification is an escalating concern in global drylands, yet assessments to guide are involved. In order to provide greater clarity, we propose that assessments of desertification and land

  3. High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53 - NF-kappaB - complex-dependent gene expression in human heart failure

    E-Print Network [OSTI]

    Choy, Mun-Kit; Movassagh, Mehregan; Siggens, Lee; Vujic, Ana; Goddard, Martin; Sanchez, Ana; Perkins, Neil; Figg, Nichola; Bennett, Martin; Carroll, Jason; Foo, Roger S.-Y.

    2010-06-14

    with this cis-element. Using high-throughput sequencing, we analyzed genome-wide binding sites for the p53-RELA complex in diseased and control human hearts and found a significant overrepresentation of the STAT3 motif. We further determined that STAT3...

  4. Quality and Membrane Treatability of the Lake Houston Water Supply 

    E-Print Network [OSTI]

    Chellam, Shankar; Sharma, Ramesh; Shetty, Grishma; Wei, Ying

    2001-10-01

    fouling rates and increase chemical cleaning intervals during surface water nanofiltration (NF) (4). Therefore, an integrated membrane system employing MF or UF pretreatment to NF is expected to be an important treatment candidate for Lake Houston water...

  5. Dysregulation of nuclear factor kappa B activity and osteopontin expression in oxidant-induced atherogenesis 

    E-Print Network [OSTI]

    Williams, Edward Spencer

    2004-09-30

    NF-?B activity is critical in the regulation of atherosclerotic vascular smooth muscle cell (vSMC) phenotypes induced following oxidative injury by allylamine. The present studies were designed to detail dysregulation of NF-?B activity...

  6. ORIGINAL PAPER P. R. Territo J. Altimiras

    E-Print Network [OSTI]

    Altimiras, Jordi

    - metamorphic climax). Surface area [8.5 mm2 at stages Nieuwkoop-Faber (NF) 33±34 to 70.2 mm2 at stages NF 50±51 (hemoglobin poisoning with carbon monoxide), whole animal surface area and volume were measured and bulk] and volume (1.8 mm3 at stages NF 33±34 to 35.7 mm3 at stages NF 50±51) measured from volu- metric analysis

  7. Publication Date 4/1/2015 FEDERAL RECREATION AREA/SITE PHONE CITY STATE WEBSITE ANNUAL SENIOR ACCESS

    E-Print Network [OSTI]

    /coconino/contact/index.shtml YES YES YES Coconino NF - Peaks & Mormon Lake District 928-526-0866 Flagstaff

  8. {ksj, ms9405, ms9504}@cis.scu.edu.tw {churen, isu}@gate.sinica.edu.tw

    E-Print Network [OSTI]

    81 976 Nf, Nf, Nf 47 4,574 T, T, I 1567 35,645 XML word, pos="100863"> pos> pos>Nh> id=1 sent id=18 sent id=21 w id=1 w id=10 w id=11 w id=15 word D pos 0200 tag1 word D pos 0200

  9. PROTON BEAM REQUIREMENTS FOR A NEUTRINO FACTORY AND MUON COLLIDER

    E-Print Network [OSTI]

    Zisman, Michael S.

    2010-01-01

    Table 1 summarizes the NF proton driver parameters obtainedboth facilities. Table 1. Proton driver requirements for arepetition frequency (Hz) Proton energy (GeV) Proton rms

  10. ER85357_Phase2_Eltron | netl.doe.gov

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    a proprietary, high-temperature nanofiltration (NF) technology (DurafluxTM) to remove salt and other dissolved solids from produced water originating from domestic oil and gas...

  11. Multicasting in Large Random Wireless Networks: Bounds on the Minimum Energy per Bit

    E-Print Network [OSTI]

    Verdú, Sergio

    was supported in part by the Office of Naval Research under contract numbers W911NF-07-1-0185 and N00014-07-1-0555

  12. 4786 IEEE TRANSACTIONS ON INFORMATION THEORY, VOL. 55, NO. 10, OCTOBER 2009 Probabilistic Coherence and Proper Scoring Rules

    E-Print Network [OSTI]

    Fitelson, Branden

    by the Office of Naval Research under Contracts W911NF-07-1-0185 and N00014-07-1-0555. J. B. Predd is with RAND

  13. Minimum Energy per Bit for Gaussian Broadcast Channels with Common Message and Cooperating

    E-Print Network [OSTI]

    Verdú, Sergio

    by the Office of Naval Research under contract numbers W911NF-07-1-0185 and N00014-07-1-0555. The minimum energy

  14. Energy-Distortion Tradeoff with Multiple Sources and Feedback

    E-Print Network [OSTI]

    Verdú, Sergio

    00014-07-1-0555, and the U.S. Army Research Ofce under Grant W911NF-07-1-0185. xed bandwidth ratio, we

  15. Control of size and charge selectivity in amphiphilic graft copolymer nanofiltration membranes

    E-Print Network [OSTI]

    Lovell, Nathan Gary

    2010-01-01

    The throughput and efficiency of membrane separations make polymer filtration membranes an important resource for the pharmaceutical, food and wastewater treatment industries. Nanofiltration (NF) membranes fill an important ...

  16. Dartmouth College HANOVER NEW HAMPSHIRE 03755-3586 Office of Pluralism and Leadership, Native American Program

    E-Print Network [OSTI]

    _campaign=DartmouthNAP&utm_content=157886900294782 976&ref=nf Registrar's Office Link to the ORC http://dartmouth.smartcatalogiq.com/2012/orc

  17. ISSN 1028-3358, Doklady Physics, 2006, Vol. 51, No. 10, pp. 569573. Pleiades Publishing, Inc., 2006. Original Russian Text E.A. Ivanova, D.A. Indeoetsev, N.F. Morozov, 2006, published in Doklady Akademii Nauk, 2006, Vol. 410, No. 6, pp. 754758.

    E-Print Network [OSTI]

    Ivanova, Elena A.

    investigation. In this paper, we discuss subtle features arising when such a method is applied in studying at the microscopic and macroscopic levels. In [1, 2], as an example, the dependences of both the Young modulus and the bending rigidities of samples were theoretically investigated for a two-dimensional single-crystal bar

  18. Research on Hakka Word Segmentation Processes in ChinesetoHakka TexttoSpeech System

    E-Print Network [OSTI]

    .66 40.67 30.87 26.65 33.04 33.04 1. 2.3.4.5. (Nep) (Nf) (Nc) (DE) (Na) (D) (VH) (COMMACATEGORY) (D) (VH) (VC) (Nc) (PERIODCATEGORY) (Nep) (Nf) (Nc) (DE) (Na) (D) (VH) (COMMACATEGORY) (D) (VH

  19. Genotoxicity of complex chemical mixtures 

    E-Print Network [OSTI]

    Phillips, Tracie Denise

    2009-05-15

    non-aqueous phase liquid from a wood treatment plant was separated into acid (AF), base (BF) and neutral fractions (NF). The NF was further enriched using column chromatography to produce a polychlorinated dinbenzo-p-dioxin (PCDD) and a polycyclic...

  20. 782 A. Y. KAPLAN ET AL. SOFIA U. TARASOVA

    E-Print Network [OSTI]

    Kaplan, Alexander

    (NF), but were guided only to look at the computer screen. It was found that during such unconscious-computer interface (BCI), color perception, electroencepha- logram (EEG), neurofeedback (NF), RGB model, spectral of many disabled people by the use of an available power computer. #12;UNCONSCIOUS OPERANT CONDITIONING

  1. High Flash-point Fluid Flow System Aerosol Flammability Study and Combustion Mechanism Analysis 

    E-Print Network [OSTI]

    Huang, Szu-Ying

    2013-12-02

    regions of three main, widely-used commercial heat transfer fluids: Paratherm NF (P-NF); Dowtherm-600 (D-600); and Plate Heat Exchange Fluid (PHE), were analyzed by electro-spray generation with laser diffraction particle analysis method. The aerosol...

  2. Harold G. Kirk Brookhaven National Laboratory

    E-Print Network [OSTI]

    McDonald, Kirk

    Harold G. Kirk Brookhaven National Laboratory The IDS-NF Target Baseline IDS-NF Plenary Meeting Rutherford Appleton Lab September 20-25, 2010 #12;Harold G. Kirk 2 The Neutrino Factory Target Concept/GeV/interactingproton) - + Palmer, PAC97 #12;Harold G. Kirk 3 The Study 2 Target System Neutrino

  3. Dmarche qualit au sein d'un laboratoire de recherche du CNRS: FEMTO-ST

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    également lancés dans la mise en place de la norme NF EN ISO 9001 afin d'obtenir une certification décennies des laboratoires accrédités par le COFRAC selon le référentiel NF EN ISO/CEI 17025. Ainsi, le : recherche, qualité, laboratoires, label Carnot, ISO 9001, ISO/CEI 17025, Université de Franche-Comté, FEMTO

  4. ASPECTS OF PARTIAL INFORMATION IN DATABASES Leonid Libkin

    E-Print Network [OSTI]

    Libkin, Leonid

    {c7 38. Kg4{f5 Nf6:d5+ 13. h2{h3? Bc8{d7 39. Kf5{g4 Nd5{f6+ 14. Rb1{c1 g7{g6 40. Kg4{f5 Nf6{g8+ 15. Nf3{d2 Ra8{b8 41. Kf5{g4 Ng8{f6+ 16. Nd2{b3 Nc5:b3 42. Kg4{f5 Nf6{g8+ 17. Qc2:b3 c6{c5 43. Kf5{g4 Be7:g5 18. Kg1{h2 Kg8{h8 44. Kg4:g5 Rf8{f7 19. Qb3{c2 Nf6{g8 45. Bf2{h4 Rh6{g6+ 20. Be2{g4 Ng8{h6 46. Kg

  5. First order switched-capacitor building blocks for analog circuits 

    E-Print Network [OSTI]

    Nguyen, Liem Thanh

    1983-01-01

    switches. (a. ) Circuit schematic and, (b. ) the resulting two-phase clock signals. 29 13 Magnitude response of the stray sensitive configuration with V3 connected to V and V2 grounded. Cl 4. 591nF, 0 C3=255. 4pF, and C4 14. 7nF, 31 14 Phase response... of the stray sensitive configuration with V3 connected to V and V2 grounded . C 1 =4 . 5 91 nF 0 C3=255. 4pP, and C4=14. 7nF. 32 15 Magnitude response of the stray sensitive configuration with V3 connected to VO and Vl grounded. Cp4. 591nF, C3=255. 4p...

  6. Influence of the Torsion Angle in 3,3'-Dimethyl-2,2'-bipyridine on the Intermediate Valence of Yb in (C5Me5)2 Yb(3,3'-Me2-bipy)

    SciTech Connect (OSTI)

    Nocton, Gré gory; Booth, Corwin H.; Maron, Laurent; Andersen, Richard A.

    2014-06-26

    The synthesis and X-ray crystal structures of Cp-2*Yb(3,3'-Me(2)bipy) and [Cp-2 Yb(3,3'-Me(2)bipy)][Cp-2 YbCl1.6I0.4]center dot CH2Cl2 are described. In both complexes, the NCCN torsion angles are approximately 40 degrees. The temperature-independent value of n(f) of 0.17 shows that the valence of ytterbium in the neutral adduct is multiconfigurational, in reasonable agreement with a CASSCF calculation that yields a n(f) value of 0.27; that is, the two configurations in the wave function are f(13)(pi(1))(1) and f(14)(pi(1))(0) in a ratio of 0.27:0.73, respectively, and the open-shell singlet lies 0.28 eV below the triplet state (n(f) accounts for f-hole occupancy; that is, n(f) = 1 when the configuration is f(13) and n(f) = 0 when the configuration is f(14)). A correlation is outlined between the value of nf and the individual ytterbocene and bipyridine fragments such that, as the reduction potentials of the ytterbocene cation and the free x,x'-R-2-bipy ligands approach each other, the value of nf and therefore the f(13):f(14) ratio reaches a maximum; conversely, the ratio is minimized as the disparity increases.

  7. Information strategies and energy conservation behavior: A meta-analysis of experimental studies from 1975 to 2012

    E-Print Network [OSTI]

    Delmas, MA; Fischlein, M; Asensio, OI

    2013-01-01

    use reminders, may render energy usage more salient and helpmore aware of their own energy usage may contribute toa relative increase in energy usage. art ic l e i nf o a b s

  8. Microsoft PowerPoint - Low Dose Update Metting 6 Dec 2012

    Broader source: Energy.gov (indexed) [DOE]

    Dose DOE's Low Dose R di ti R h R di ti R h Radiation Research Radiation Research Program Program g g NF Metting, Sc.D., Program Manager Nuclear Energy Advisory Committee Meeting...

  9. Politeness in Japanese Sign Language (JSL): Polite JSL expression as evidence for intermodal language contact influence

    E-Print Network [OSTI]

    George, Johnny Earl

    2011-01-01

    Do you mind’ Ni=#N= phrase initial nod F=chin-forward Nf=N#=phrase final nod O= O=ONEGAI ‘please’ E=polite grimacepoints to indicate, head nods and shakes to modulate their

  10. Design considerations for a solar-powered desalination system for remote communities in Australia 

    E-Print Network [OSTI]

    Richards, B.S.; Schäfer, Andrea

    2002-01-01

    Water in many areas of Australia is scarce and of poor quality. In some areas high levels of treatment are required either due to contamination of waters or due to high salinity. Nanofiltration (NF) and low-pressure reverse ...

  11. 1992 CBECS C & E

    U.S. Energy Information Administration (EIA) Indexed Site

    of Natural Gas by End Use, 1989 Natural Gas Consumption (trillion Btu) Space Water a Total Heating Heating Cooking Other RSE Building Row Characteristics Factor 1.0 NF...

  12. Systematic approach for simultaneously correcting the band-gap...

    Office of Scientific and Technical Information (OSTI)

    Number: Army W911NF-10-1-0524; AC02-05CH11231 Type: Publisher's Accepted Manuscript Journal Name: Physical Review. B, Condensed Matter and Materials Physics Additional Journal...

  13. gropuUCSB2013-07-30_Webinar.ppt

    Broader source: Energy.gov (indexed) [DOE]

    124345 1-D profile through NF PM2 Summary Cont. - APT Fe TA artifact L T 40115 kgmm 2 PM2 Summary Cont. - Tensile Strength Property L T * Excellent low transition...

  14. Anti-inflammatory Effect of Vigna Unguiculata Polyphenols in Raw 264.7 Macrophages 

    E-Print Network [OSTI]

    Siska, Karla P

    2013-04-29

    for phenolic content and composition using UV-Visible Spectroscopy and HPLC; antioxidant activation mechanism (AOX) by oxygen radical absorbance capacity (ORAC). Anti-inflammatory activity was measured via NF-?B activation in Raw 264.7 macrophages challenged...

  15. Indirect Determination of the 230Th(n,f) and 231Th(n,f) Cross Sections for Thorium-Based Nuclear Energy Systems

    E-Print Network [OSTI]

    Stroberg, S.R.

    2012-01-01

    n,f) Cross Sections for Thorium-Based Nuclear Energy Systemsf ) Cross Sections for Thorium-Based Nuclear Energy Systemsbenchmark- ing of advanced thorium-based nuclear reactor

  16. YEAR

    National Nuclear Security Administration (NNSA)

    300 YEAR 2011 Males 109 Females 191 YEAR 2011 SES 9 EJEK 1 NN (Engineering) 2 NQ (ProfTechAdmin) 203 NU (TechAdmin Support) 38 NF (Future Ldrs) 47 YEAR 2011 American Indian...

  17. YEAR

    National Nuclear Security Administration (NNSA)

    86 YEAR 2012 Males 103 Females 183 YEAR 2012 SES 7 EJEK 1 NN (Engineering) 1 NQ (ProfTechAdmin) 202 NU (TechAdmin Support) 30 NF (Future Ldrs) 45 YEAR 2012 American Indian Male...

  18. Adsorption of Estrone on nanofiltration and reverse osmosis membranes in water and wastewater treatment 

    E-Print Network [OSTI]

    Nghiem, D.L.; Schäfer, Andrea; Waite, T.D.

    2002-01-01

    Adsorption of the trace contaminant estrone, a natural hormone and commonly abundant in surface waters and in treated as well as untreated wastewaters, to eight commercial nanofiltration (NF) and reverse osmosis (RO) ...

  19. Renewable energy powered membrane technology. 2. The effect of energy fluctuations on performance of a photovoltaic hybrid membrane system 

    E-Print Network [OSTI]

    Richards, B.S.; Capão, D.P.S.; Schäfer, Andrea

    2008-01-01

    This paper reports on the performance fluctuations during the operation of a batteryless hybrid ultrafiltration – nanofiltration / reverse osmosis (UF-NF/RO) membrane desalination system powered by photovoltaics treating ...

  20. Impact of pH on the removal of fluoride, nitrate and boron by nanofiltration/reverse osmosis 

    E-Print Network [OSTI]

    Richards, Laura A.; Vuachère, Marion; Schäfer, Andrea

    2010-01-01

    The objective of this study was to evaluate the impact of pH on boron, fluoride, and nitrate retention by comparing modelled speciation predictions with retention using six different nanofiltration (NF) and reverse osmosis ...

  1. Thermal transition temperature from twisted mass QCD

    E-Print Network [OSTI]

    Florian Burger; Ernst-Michael Ilgenfritz; Malik Kirchner; Maria Paola Lombardo; Michael Müller-Preussker; Owe Philipsen; Carsten Urbach; Lars Zeidlewicz

    2010-09-20

    We present the current status of lattice simulations with N_f=2 maximally twisted mass Wilson fermions at finite temperature. In particular, the determination of the thermal transition temperature is discussed.

  2. Removal mechanisms of organic and inorganic solutes in raw, upland drinking water by nanofiltration: influence of solute-solute and solute-membrane interactions 

    E-Print Network [OSTI]

    De Munari, Annalisa; Munari, Annalisa de

    2012-11-29

    Nanofiltration (NF) membranes have been applied successfully for the removal of inorganic and organic pollutants, including micropollutants, from drinking water for the past two decades. However, a complete and quantitative ...

  3. Decay Studies of the New Isotopes Hf-162,hf-163 

    E-Print Network [OSTI]

    Schrewe, U. J.; Hagberg, E.; Schmeing, H.; Hardy, John C.; Koslowsky, V. T.; Sharma, KS; Clifford, E. T. H.

    1982-01-01

    ) considering interaction between a hydraulic fracture (HF) and a pre-existing NF, has been investigated comprehensively using a two dimensional Displacement Discontinuity Method (DDM) Model in this thesis. The rock is first considered as an elastic impermeable...

  4. Phonetic and Cognitive Bases of Sound Change

    E-Print Network [OSTI]

    Kataoka, Reiko

    2011-01-01

    heed, hid, head, had, hot, HUD, hood, and who’d. Data ofin a reference word who’d (/hud/) in fast, medium, and slowof /u/ in who’d (/hud/) .47 3.12 Plots of NF2

  5. Sythhesis and Optimization of Hybrid Membrane Desalination Networks with Value Extraction 

    E-Print Network [OSTI]

    AlNouss, Ahmed M

    2014-02-04

    Membrane desalination technology has become a valuable advanced water treatment process to purify difficult water sources for potable use. Reverse Osmosis (RO) and Nanofiltration (NF) processes are commonly used desalination technologies. Studies...

  6. Monopole Taxonomy in Three-Dimensional Conformal Field Theories

    E-Print Network [OSTI]

    Dyer, Ethan; Pufu, Silviu S

    2013-01-01

    We study monopole operators at the infrared fixed points of Abelian and non-Abelian gauge theories with N_f fermion flavors in three dimensions. At large N_f, independent monopole operators can be defined via the state-operator correspondence only for stable monopole backgrounds. In Abelian theories, every monopole background is stable. In the non-Abelian case, we find that many (but not all) backgrounds are stable in each topological class. We calculate the infrared scaling dimensions of the corresponding operators through next-to-leading order in 1/N_f. In the case of U(N_c) QCD with N_f fundamental fermions (and in particular in the QED case, N_c =1), we find that the monopole operators transform as non-trivial irreducible representations of the SU(N_f) flavor symmetry group.

  7. Design and synthesis of probes for detection of protein-protein interaction and RNA localization

    E-Print Network [OSTI]

    Ryan, Jeremy Adam

    2005-01-01

    The use of the ketone biotin - benzophenone-biotin hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB p50 dimers was assessed. A series of benzophenone-based probes were synthesized and ...

  8. The Mobile Test and Demonstration Unit, A Cooperative Project Between EPRI, Utilities and Industry to Demonstrate New Water Treatment Technologies 

    E-Print Network [OSTI]

    Strasser, J.; Mannapperuma, J.

    1995-01-01

    and has demonstrated that membrane processes like MF, UF, NF and RO can successfully be applied to remove BOD and TSS from process streams, often recovering valuable solids, reducing sewer charges and meeting environmental regulations....

  9. Interleukin-23 and interleukin-17: Importance in pathogenesis and therapy of psoriasis

    E-Print Network [OSTI]

    Mudigonda, Parvathi; Mudigonda, Tejaswi; Feneran, Ashley N; Alamdari, Habibollah S; Sandoval, Laura; Feldman, Steven R

    2012-01-01

    reveals association of psoriasis with IL-23 and NF-kappaBlinks between IL-23 and psoriasis. J Invest Dermatol . MayImmunopathogenesis of psoriasis. Clin Rev Allergy Immunol .

  10. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    MILC asqtad QCD SU(3) gauge ensemble; series=a; a=0.11fm minus 0.0022fm ; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  11. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050b

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    MILC asqtad QCD SU(3) gauge ensemble; series=b; a=0.11fm minus 0.0022fm; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  12. Removal of adsorbing estrogenic micropollutants by nanofiltration membranes in cross-flow – experiments and model development 

    E-Print Network [OSTI]

    Semião, Andrea J.C.

    2011-11-22

    Nanofiltration (NF) can be used in water and wastewater treatment as well as water recycling applications, removing micropollutants such as hormones. Due to their potential health risk it is vital to understand their ...

  13. Category Theoretic Analysis of Hierarchical Protein Materials and Social Networks

    E-Print Network [OSTI]

    Spivak, David

    the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution acknowledge support from a DOD-PECASE award (N000141010562) and DOD-MURI (W911NF0910541). DIS acknowledges

  14. Subject:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    rotation reversals, poloidal impurity asymmetries and "non-local" heat transport Gao thesis 2015, Rice NF 2013). We are not yet certain if our RF L-mode target plasmas will...

  15. Numerical Investigation of Interaction Between Hydraulic Fractures and Natural Fractures 

    E-Print Network [OSTI]

    Xue, Wenxu

    2011-02-22

    Hydraulic fracturing of a naturally-fractured reservoir is a challenge for industry, as fractures can have complex growth patterns when propagating in systems of natural fractures in the reservoir. Fracture propagation near a natural fracture (NF...

  16. Impact of Information Loss and Information Error on Network-enabled Decision-making

    E-Print Network [OSTI]

    by the Network Science Collaborative Technology Alliance: No. W911NF08R0013 from the Army Research Lab.................................................................... 2 1.1.2 Scenario Two ­ Deepwater Horizon oil spill (pre

  17. Quantitative mouse renal perfusion using arterial spin labeling

    E-Print Network [OSTI]

    Rajendran, R; Lew, SK; Yong, CX; Tan, J; Wang, DJJ; Chuang, KH

    2013-01-01

    NF, Graf H, ClaussenCD, Schick F. Magnetic resonanceNI, Kramer U, Claussen CD, Schick F. Perfusion MR imagingH, Claussen CD, Schlemmer HP, Schick F. High resolution MR

  18. A multi-scale approach to statistical and model-based structural health monitoring with application to embedded sensing for wind energy

    E-Print Network [OSTI]

    Taylor, Stuart Glynn

    2013-01-01

    of Wind Turbine Blades Using Piezoelectric Active-Sensors,"Sensor Node to Health Monitoring of Operational Wind TurbineSensors Capacitance % Change (nF) 3.4.4. Deployment Example: Wind Turbine

  19. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050b

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    2015-01-01

    MILC asqtad QCD SU(3) gauge ensemble; series=b; a=0.11fm minus 0.0022fm; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  20. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    2015-01-01

    MILC asqtad QCD SU(3) gauge ensemble; series=a; a=0.11fm minus 0.0022fm ; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  1. Systems and methods for treating material

    DOE Patents [OSTI]

    Scheele, Randall D; McNamara, Bruce K

    2014-10-21

    Systems for treating material are provided that can include a vessel defining a volume, at least one conduit coupled to the vessel and in fluid communication with the vessel, material within the vessel, and NF.sub.3 material within the conduit. Methods for fluorinating material are provided that can include exposing the material to NF.sub.3 to fluorinate at least a portion of the material. Methods for separating components of material are also provided that can include exposing the material to NF.sub.3 to at least partially fluorinate a portion of the material, and separating at least one fluorinated component of the fluorinated portion from the material. The materials exposed to the NF.sub.3 material can include but are not limited to one or more of U, Ru, Rh, Mo, Tc, Np, Pu, Sb, Ag, Am, Sn, Zr, Cs, Th, and/or Rb.

  2. Bioremediation of contaminated groundwater

    DOE Patents [OSTI]

    Hazen, T.C.; Fliermans, C.B.

    1994-01-01

    Disclosed is an apparatus and method for in situ remediation of contaminated subsurface soil or groundwater contaminated by chlorinated hydrocarbons. A nutrient fluid (NF) is selected to simulated the growth and reproduction of indigenous subsurface microorganisms capable of degrading the contaminants; an oxygenated fluid (OF) is selected to create an aerobic environment with anaerobic pockets. NF is injected periodically while OF is injected continuously and both are extracted so that both are drawn across the plume. NF stimulates microbial colony growth; withholding it periodically forces the larger, healthy colony of microbes to degrade the contaminants. Treatment is continued until the subsurface concentration of contaminants is acceptable. NF can be methane and OF be air, for stimulating production of methanotrophs to break down chlorohydrocarbons, especially TCE and tetrachloroethylene.

  3. Chemical Addition prior to Membrane Processes for Natural Organic Matter (NOM) Removal 

    E-Print Network [OSTI]

    Schäfer, Andrea; Fane, Anthony G.; Waite, T. D.

    1998-01-01

    Membrane processes for surface water treatment include microfiltration (MF), ultrafiltration (UF) and nanofiltration (NF), depending on the target material to be removed and the limiting process economics. MF will remove ...

  4. Nucleophilic fluorination of aromatic compounds

    DOE Patents [OSTI]

    Satyamurthy, Nagichettiar; Barrio, Jorge R

    2014-03-18

    Iodylbenzene derivatives substituted with electron donating as well as electron withdrawing groups on the aromatic ring are used as precursors in aromatic nucleophilic substitution reactions. The iodyl group (IO.sub.2) is regiospecifically substituted by nucleophilic fluoride to provide the corresponding fluoroaryl derivatives. No-carrier-added [F-18]fluoride ion derived from anhydrous [F-18](F/Kryptofix, [F-18]CsF or a quaternary ammonium fluoride (e.g., Me.sub.4NF, Et.sub.4NF, n-Bu.sub.4NF, (PhCH.sub.2).sub.4NF) exclusively substitutes the iodyl moiety in these derivatives and provides high specific activity F-18 labeled fluoroaryl analogs. Iodyl derivatives of a benzothiazole analog and 6-iodyl-L-dopa derivatives have been synthesized as precursors and have been used in the preparation of no-carrier-added [F-18]fluorobenzothiazole as well as 6-[F-18]fluoro-L-dopa.

  5. Structural studies of magnesium nitride fluorides by powder neutron diffraction

    SciTech Connect (OSTI)

    Brogan, Michael A.; Hughes, Robert W.; Smith, Ronald I.; Gregory, Duncan H.

    2012-01-15

    Samples of ternary nitride fluorides, Mg{sub 3}NF{sub 3} and Mg{sub 2}NF have been prepared by solid state reaction of Mg{sub 3}N{sub 2} and MgF{sub 2} at 1323-1423 K and investigated by powder X-ray and powder neutron diffraction techniques. Mg{sub 3}NF{sub 3} is cubic (space group: Pm3m) and has a structure related to rock-salt MgO, but with one cation site vacant. Mg{sub 2}NF is tetragonal (space group: I4{sub 1}/amd) and has an anti-LiFeO{sub 2} related structure. Both compounds are essentially ionic and form structures in which nitride and fluoride anions are crystallographically ordered. The nitride fluorides show temperature independent paramagnetic behaviour between 5 and 300 K. - Graphical abstract: Definitive structures of the ternary magnesium nitride fluorides Mg{sub 3}NF{sub 3} and the lower temperature polymorph of Mg{sub 2}NF have been determined from powder neutron diffraction data. The nitride halides are essentially ionic and exhibit weak temperature independent paramagnetic behaviour. Highlights: Black-Right-Pointing-Pointer Definitive structures of Mg{sub 3}NF{sub 3} and Mg{sub 2}NF were determined by neutron diffraction. Black-Right-Pointing-Pointer Nitride and fluoride anions are crystallographically ordered in both structures. Black-Right-Pointing-Pointer Both compounds exhibit weak, temperature independent paramagnetic behaviour. Black-Right-Pointing-Pointer The compounds are essentially ionic with ionicity increasing with F{sup -} content.

  6. The Elimination of Steam Traps 

    E-Print Network [OSTI]

    Dickman, F.

    1985-01-01

    compile published data by three leading steam trap facturers. ANNUAL COST OF STEAM LOSS FOR 100 PSIG STEAM AT $5/1000 LBS. TgpOrlflce l18nul8ctuNf M.,utectu,., DI.mNr A' 84 1/." . $ 3,150 $ 2,313 e to from nu ufKluNf co 3,1711 1/4" $12,eoo $ 9...

  7. Anomalous magnetic behavior in nanocomposite materials of reduced graphene oxide-Ni/NiFe{sub 2}O{sub 4}

    SciTech Connect (OSTI)

    Kollu, Pratap E-mail: anirmalagrace@vit.ac.in; Prathapani, Sateesh; Varaprasadarao, Eswara K.; Mallick, Sudhanshu; Bahadur, D. E-mail: anirmalagrace@vit.ac.in; Santosh, Chella; Grace, Andrews Nirmala E-mail: anirmalagrace@vit.ac.in

    2014-08-04

    Magnetic Reduced Graphene Oxide-Nickel/NiFe{sub 2}O{sub 4} (RGO-Ni/NF) nanocomposite has been synthesized by one pot solvothermal method. Respective phase formations and their purities in the composite are confirmed by High Resolution Transmission Electron Microscope and X Ray Diffraction, respectively. For the RGO-Ni/NF composite material finite-size effects lead to the anomalous magnetic behavior, which is corroborated in temperature and field dependent magnetization curves. Here, we are reporting the behavior of higher magnetization values for Zero Field Cooled condition to that of Field Cooled for the RGO-Ni/NF nanocomposite. Also, the observed negative and positive moments in Hysteresis loops at relatively smaller applied fields (100?Oe and 200?Oe) are explained on the basis of surface spin disorder.

  8. Large deviation for diffusions and Hamilton-Jacobi equation in Hilbert spaces

    E-Print Network [OSTI]

    Feng, Jin

    2006-01-01

    . Then for each f ? Cb(S) (bounded continuous functions on S), if we define ?n(f ) = n?1 logE[exp{nf (Xn)}], lim n?+? ?n(f ) = lim n?+? 1 n logE [ enf (Xn) ] = sup x?S {f (x) ? I (x)} = ?(f ).(1.1) LARGE DEVIATIONS IN HILBERT SPACE 323 (b) Suppose that {Xn...} is exponentially tight (Definition 1.17) and that the limit (1.1) exists for each f ? Cb(S). Then {Xn} satisfies the large deviation with good rate function I (x) = sup f ?Cb(S) ( f (x) ? ?(f ) ) .(1.2) See Theorems 4.3.1 and 4.4.2 in [12]. The main result in [18...

  9. Nuclear saturation in lowest-order Brueckner theory with two- and three-nucleon forces in view of chiral effective field theory

    E-Print Network [OSTI]

    Kohno, M

    2015-01-01

    The nuclear saturation mechanism is discussed in terms of two-nucleon and three-nucleon interactions in chiral effective field theory (Ch-EFT), using the framework of lowest-order Brueckner theory. After the Coester band, which is observed in calculating saturation points with various nucleon-nucleon (NN) forces, is revisited using modern NN potentials and their low-momentum equivalent interactions, detailed account of the saturation curve of the Ch-EFT interaction is presented. The three-nucleon force (3NF) is treated by reducing it to an effective two-body interaction by folding the third nucleon degrees of freedom. Uncertainties due to the choice of the 3NF low-energy constants $c_D$ and $c_E$ are discussed. The reduction of the cutoff-energy dependence of the NN potential is explained by demonstrating the effect of the 3NF in the $^1$S$_0$ and $^3$S$_1$ states.

  10. Report on thermal aging effects on tensile properties of ferritic-martensitic steels.

    SciTech Connect (OSTI)

    Li, M.; Soppet, W.K.; Rink, D.L.; Listwan, J.T.; Natesan, K.

    2012-05-10

    This report provides an update on the evaluation of thermal-aging induced degradation of tensile properties of advanced ferritic-martensitic steels. The report is the first deliverable (level 3) in FY11 (M3A11AN04030103), under the Work Package A-11AN040301, 'Advanced Alloy Testing' performed by Argonne National Laboratory, as part of Advanced Structural Materials Program for the Advanced Reactor Concepts. This work package supports the advanced structural materials development by providing tensile data on aged alloys and a mechanistic model, validated by experiments, with a predictive capability on long-term performance. The scope of work is to evaluate the effect of thermal aging on the tensile properties of advanced alloys such as ferritic-martensitic steels, mod.9Cr-1Mo, NF616, and advanced austenitic stainless steel, HT-UPS. The aging experiments have been conducted over a temperature of 550-750 C for various time periods to simulate the microstructural changes in the alloys as a function of time at temperature. In addition, a mechanistic model based on thermodynamics and kinetics has been used to address the changes in microstructure of the alloys as a function of time and temperature, which is developed in the companion work package at ANL. The focus of this project is advanced alloy testing and understanding the effects of long-term thermal aging on the tensile properties. Advanced materials examined in this project include ferritic-martensitic steels mod.9Cr-1Mo and NF616, and austenitic steel, HT-UPS. The report summarizes the tensile testing results of thermally-aged mod.9Cr-1Mo, NF616 H1 and NF616 H2 ferritic-martensitic steels. NF616 H1 and NF616 H2 experienced different thermal-mechanical treatments before thermal aging experiments. NF616 H1 was normalized and tempered, and NF616 H2 was normalized and tempered and cold-rolled. By examining these two heats, we evaluated the effects of thermal-mechanical treatments on material microstructures and associated mechanical properties during long-term aging at elevated temperatures. Thermal aging experiments at different temperatures and periods of time have been completed: 550 C for up to 5000 h, 600 C for up to 7500 h, and 650 C for more than 10,000 h. Tensile properties were measured on thermally aged specimens and aging effect on tensile behavior was assessed. Effects of thermal aging on deformation and failure mechanisms were investigated by using in-situ straining technique with simultaneous synchrotron XRD measurements.

  11. IEA W-GIFT-6 SMALL SPECIMEN TEST TECHNIQUE

    E-Print Network [OSTI]

    McDonald, Kirk

    IEA W-GIFT-6 SMALL SPECIMEN TEST TECHNIQUE UNDER IFMIF/EVEDA PROJECT OF BA FOR FATIGUE AND FRACTURE,t[%] Number of cycles to failure, Nf F82H-IEA HG-1.25 HG-1.25 (Hirose, Kim) RB-1 RB-7 RB-4, -7 RB-7, -10 of cycles to failure, Nf JLF-1 HG-1.25 HG-1.25 HG-6 (Hirose) RB-1 RB-8 (Nishimura&Li) F82H-IEA (Grain size

  12. Design Considerations for Future RF Circuits 1 Behzad Razavi

    E-Print Network [OSTI]

    Razavi, Behzad

    . I. INTRODUCTION The evolution of RF circuits has crossed an inflection point defined by two Current: 2 mA Conversion Gain: 5.4 dB NF: 8.2 dB IIP3: -3.8 dBm Supply Current: 2 mA Conversion Gain: 15 dB NF: 8.1 dB IIP3: +3.0 dBm 1 2 1 2 Fig. 1. (a) Active and (b) passive mixers operating with a 1-V

  13. K semileptonic form factor with HISQ fermions at the physical point

    E-Print Network [OSTI]

    E. Gámiz; A. Bazavov; C. Bernard; C. Bouchard; C. DeTar; D. Du; A. X. El-Khadra; J. Foley; E. D. Freeland; Steven Gottlieb; U. M. Heller; J. Kim; A. S. Kronfeld; J. Laiho; L. Levkova; P. B. Mackenzie; E. T. Neil; M. B. Oktay; Si-Wei Qiu; J. N. Simone; R. Sugar; D. Toussaint; R. S. Van de Water; Ran Zhou

    2014-08-17

    We present results for the form factor $f_+^{K \\pi}(0)$, needed to extract the CKM matrix element $|V_{us}|$ from experimental data on semileptonic $K$ decays, on the HISQ $N_f=2+1+1$ MILC configurations. The HISQ action is also used for the valence sector. The data set used for our final result includes three different values of the lattice spacing and data at the physical light quark masses. We discuss the error budget and how this calculation improves on our previous determination of $f_+^{K \\pi}(0)$ on the asqtad $N_f=2+1$ MILC configurations.

  14. Can 3-D models explain the observed fractions of fossil and non-fossil carbon in and near Mexico City?

    SciTech Connect (OSTI)

    Hodzic, Alma; Jimenez, Jose L.; Prevot, A. S. H.; Szidat, S.; Fast, Jerome D.; Madronich, Sasha

    2010-11-25

    Abstract. A 3-D chemistry-transport model has been applied to the Mexico City metropolitan area to investigate the origin of elevated levels of non-fossil (NF) carbonaceous aerosols observed in this highly urbanized region. High time resolution measurements of the fine aerosol concentration and composition, and 12 or 24 h integrated 14C measurements of aerosol modern carbon have been performed in and near Mexico City during the March 2006 MILAGRO field experiment. The non-fossil carbon fraction (fNF), which is lower than the measured modern fraction (fM) due to the elevated 14C in the atmosphere caused by nuclear bomb testing, is estimated from the measured fM and the source-dependent information on modern carbon enrichment. The fNF contained in PM1 total carbon analyzed by a US team (f TC NF ) ranged from 0.37 to 0.67 at the downtown location, and from 0.50 to 0.86 at the suburban site. Substantially lower values (i.e. 0.24–0.49) were found for PM10 filters downtown by an independent set of measurements (Swiss team), which are inconsistent with the modeled and known differences between the size ranges, suggesting higher than expected uncertainties in the measurement techniques of 14C. An increase in the non-fossil organic carbon (OC) fraction (f OC NF ) by 0.10–0.15 was observed for both sets of filters during periods with enhanced wildfire activity in comparison to periods when fires were suppressed by rain, which is consistent with the wildfire impacts estimated with other methods. Model results show that the relatively high fraction of nonfossil carbon found in Mexico City seems to arise from the combination in about equal proportions of regional biogenic SOA, biomass burning POA and SOA, as well as non-fossil urban POA and SOA. Predicted spatial and temporal variations for f OC NF are similar to those in the measurements between the urban vs. suburban sites, and high-fire vs. low-fire periods. The absolute modeled values of f OC NF are consistent with the Swiss dataset but lower than the US dataset. Resolving the 14C measurement discrepancies is necessary for further progress in model evaluation. The model simulations that included secondary organic aerosol (SOA) formation from semi-volatile and intermediate volatility (S/IVOC) vapors showed improved closure for the total OA mass compared to simulations which only included SOA from VOCs, providing a more realistic basis to evaluate the fNF predictions. f OC NF urban sources of modern carbon are important in reducing or removing the difference in fNF between model and measurements, even though they are often neglected on the interpretation of 14C datasets. An underprediction of biomass burning POA by the model during some mornings also explains a part of the model-measurement differences. The fNF of urban POA and SOA precursors is an important parameter that needs to be better constrained by measurements. Performing faster ( 3 h) 14C measurements in future campaigns is critical to further progress in this area. To our knowledge this is the first time that radiocarbon measurements are used together with aerosol mass spectrometer (AMS) organic components to assess the performance of a regional model for organic aerosols.

  15. Cell 125, May 19, 2006 2006 Elsevier Inc. 645 responsible for the proliferation of

    E-Print Network [OSTI]

    .g., ginseng extract and green-tea extract), all of which can inhibit activation of NF-B. These antiRences Bignell, G.R., Warren, W., Seal, S., Takahashi, M., Rapley, E., Barfoot, R., Green, H., Brown, C., Biggs

  16. Engineering Radio resource management for OFDMA systems under practical

    E-Print Network [OSTI]

    Martin, Ralph R.

    in the construction industry / Bin Chen. Ph.D. 2009 NF nanofiltration membrane characterization / Ahmed Saleh Al verification / Neil Pearson. M.Phil. 2008 #12; Design and optimisation of joints in a carbon fibre bicycle reservoir 'A' / Hussain Al-Hassani. M.Res. 2008 Chemical Engineering Tensile properties of polymeric

  17. Nucleon generalized form factors and sigma term from lattice QCD near the physical quark mass

    E-Print Network [OSTI]

    G. S. Bali; S. Collins; B. Gläßle; M. Göckeler; J. Najjar; R. Rödl; A. Schäfer; R. Schiel; W. Söldner; A. Sternbeck; P. Wein

    2013-12-03

    We present new N_f=2 data for the nucleon generalized form factors, varying volume, lattice spacing and pion mass, down to 150 MeV. We also give an update of our direct calculation of the nucleon sigma term for a range of pion mass values including the lightest one.

  18. CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE DOI: 10.1177/0022034511421200

    E-Print Network [OSTI]

    Cooper, Robin L.

    mechanisms of tobacco-induced disease and may identify novel therapeutic approaches for the management, interleukin; LPS, lipopolysaccharide; MAPK, mitogen-activated protein kinase; NF-B, nuclear factor kappa). Despite widespread knowledge of the risks posed by CS, the worldwide prevalence of tobacco use

  19. DESIGNER WATER Dr. Torleiv Bilstad

    E-Print Network [OSTI]

    Treatment #12;Produced Water: Separation and polishing Choke technology Separator technology Oil Droplet water - Produced by adjusting the ionic composition of the injected seawater thereby modifying = 34500 ppm F2. Produced Water with very high TDS as feed Type NF membrane ­ NANO - BW 4040 #12;Pictures

  20. Systematic and Scalable Testing of Concurrent Programs

    E-Print Network [OSTI]

    award number W911NF-09-1-0273, the U.S. Department of Energy under award number DE-FC02-06ER25767 (PDSI Consortium: Actifio, American Power Conversion, EMC Corporation, Facebook, Fusion-io, Google Hewlett, scaling systematic testing to real-world programs of unprecedented complexity. #12;#12;Acknowledgments

  1. The effects of ethylenediurea and sodium erythorbate on photosynthetic function of ozone-exposed loblolly pine seedlings 

    E-Print Network [OSTI]

    Kuehler, Eric Anthony

    1995-01-01

    -top chambers in east Texas for one growing season beginning in April 1994 while being exposed to either sub-ambient (CF), approximate ambient (NF), 1.5Y,, 2.OX, or 2.5X ambient ozone levels. Net photosynthesis (A), stomatal conductance (g), and chloroplast...

  2. The effect of grazing management systems on performance of beef animals and on the yield and chemical and botanical composition of Texas coast prairie pastures 

    E-Print Network [OSTI]

    Riewe, Marvin Edmund

    1959-01-01

    of the forage during the first part of the grazing season followed by 'a rise during the latter part resulted in a highly significant interaction of time with period. N~lt -8 - W *e ? Th ight*d v g nit og n-f extract (NFE) content of the available forage...

  3. Rafael Molina Mtodos avanzados de estimacin de movimiento

    E-Print Network [OSTI]

    Granada, Universidad de

    ,y,t), al igual que hemos hecho con fx, fy y ft ) y los subíndices indican derivadas parciales. Esta incertidumbre Para justificar su modelo, Simoncelli considera que no disponemos de las derivadas temporales sino~n análogamente podemos suponer que la derivada temporal que hemos observado satisface 2nf ~ f tt -= donde n2 es

  4. Rafael Molina Mtodos avanzados de estimacin de movimiento

    E-Print Network [OSTI]

    Granada, Universidad de

    ,y,t), al igual que hemos hecho con fx, fy y ft ) y los subíndices indican derivadas parciales. Esta incertidumbre Para justificar su modelo, Simoncelli considera que no disponemos de las derivadas temporales sino~n análogamente podemos suponer que la derivada temporal que hemos observado satisface ~ f 2nf ~ f tt -= donde n2

  5. Tomographic reconstruction of tokamak plasma light emission from single image using wavelet-vaguelette decomposition

    E-Print Network [OSTI]

    Schneider, Kai

    Tomographic reconstruction of tokamak plasma light emission from single image using wavelet.1088/0029-5515/52/1/013005 Tomographic reconstruction of tokamak plasma light emission from single image using wavelet.iop.org/NF/52/013005 Abstract Images acquired by cameras installed in tokamaks are difficult to interpret

  6. 12/31/11 Analog to Digital Converter Test Report Page 1 of 5 Created 3/3/08 by Nicole Rodia Modified 5/18/08 11:01 PM by Nicole Rodia

    E-Print Network [OSTI]

    Ruina, Andy L.

    as well as on the Ko Brain board under a variety of conditions. Using the Ko Brain board, tests were wire Soldering iron 1.5 V battery Battery holder 10 k, 100 k resistors 22 nF capacitor Multimeter wrap wire to keep wires short to reduce electromagnetic interference. Soldered connections to battery

  7. Molecular Cell, Volume 50 Supplemental Information

    E-Print Network [OSTI]

    Komander, David

    point mutants on NF-B activity at different concentration. (B) HA-IP and inputs on U2OS/NOD2 cells to show the low levels of NOD2 expressed under non-induced conditions. (C) Immunofluorescence analysis of U2OS/NOD2 cells with and without transfected vector expressing the HA-tag shows that the level

  8. Electromagnetic corrections to light hadron masses

    E-Print Network [OSTI]

    A. Portelli; S. Dürr; Z. Fodor; J. Frison; C. Hoelbling; S. D. Katz; S. Krieg; T. Kurth; L. Lellouch; T. Lippert; K. K. Szabó; A. Ramos

    2011-01-12

    At the precision reached in current lattice QCD calculations, electromagnetic effects are becoming numerically relevant. We will present preliminary results for electromagnetic corrections to light hadron masses, based on simulations in which a $\\mathrm{U}(1)$ degree of freedom is superimposed on $N_f=2+1$ QCD configurations from the BMW collaboration.

  9. Corrosion of ferriticmartensitic steels in steam and supercritical water Jeremy Bischoff a,b

    E-Print Network [OSTI]

    Motta, Arthur T.

    Corrosion of ferritic­martensitic steels in steam and supercritical water Jeremy Bischoff a of their resistance to radiation degradation and stress corrosion cracking, ferritic­martensitic steels such as HCM12A °C for two ferritic­martensitic alloys: HCM12A and NF616. The corrosion kinetics for the two alloys

  10. This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research

    E-Print Network [OSTI]

    Motta, Arthur T.

    a b s t r a c t NF616 is a nominal 9Cr ferritic­martensitic steel that is amongst the primary- posed nuclear energy system. Ferritic­martensitic (F­M) alloys offer high resistance to irradi- ation

  11. Corrosion of Ferritic-Martensitic Steels in Steam and Supercritical Water Jeremy Bischoffa, Arthur T. Mottaa, Chad Eichfeldb, Robert J. Comstockc, Guoping Caod, Todd

    E-Print Network [OSTI]

    Motta, Arthur T.

    Corrosion of Ferritic-Martensitic Steels in Steam and Supercritical Water Jeremy Bischoffa, Arthur, ferritic- martensitic steels such as HCM12A and NF616, are two of the candidate materials, for this reactor Corrosion tests were performed in steam and supercritical water at 500°C for two ferritic- martensitic

  12. 2010NatureAmerica,Inc.Allrightsreserved. nature immunology aDVaNCE ONLINE PUBLICaTION

    E-Print Network [OSTI]

    Nizet, Victor

    , often destructive, inflammatory processes8. Like other blood cells, neutrophils originate from self-renewing-term hematopoietic stem cells that have limited self-renewal capacity and give rise to multi- potent progenitors9-promoting cytokines14. However, activation of NF-B is also known to be critical for innate and adaptive immu- nity15

  13. Animal Model and INLA Ingelin Steinsland & Anna Marie Holand

    E-Print Network [OSTI]

    Steinsland, Ingelin

    , islands The house sparrow (Passer domesticus) is a small passerine bird Sexually dimorphic Widely spread.e. by the corresponding pedigree. #12;Model, one bird For bird i (i = 1, . . . , 3373): yi = nf f =1 f ,l(i) + ui + i , yi all fledglings are marked and blood sample collected in the nest. Adult and fledged juveniles (birds

  14. Adrenaline promotes cell proliferation and increases chemoresistance in colon cancer HT29 cells through induction of miR-155

    SciTech Connect (OSTI)

    Pu, Jun [Department of General Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038 (China)] [Department of General Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038 (China); Bai, Danna [Department of Cardiology, 323 Hospital of PLA, Xi'an 710054 (China)] [Department of Cardiology, 323 Hospital of PLA, Xi'an 710054 (China); Yang, Xia [Department of Teaching and Medical Administration, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038 (China)] [Department of Teaching and Medical Administration, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038 (China); Lu, Xiaozhao [Department of Nephrology, The 323 Hospital of PLA, Xi'an 710054 (China)] [Department of Nephrology, The 323 Hospital of PLA, Xi'an 710054 (China); Xu, Lijuan, E-mail: 13609296272@163.com [Department of Nephrology, The 323 Hospital of PLA, Xi'an 710054 (China)] [Department of Nephrology, The 323 Hospital of PLA, Xi'an 710054 (China); Lu, Jianguo, E-mail: lujianguo029@yahoo.com.cn [Department of General Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038 (China)] [Department of General Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi'an 710038 (China)

    2012-11-16

    Highlights: Black-Right-Pointing-Pointer Adrenaline increases colon cancer cell proliferation and its resistance to cisplatin. Black-Right-Pointing-Pointer Adrenaline activates NF{kappa}B in a dose dependent manner. Black-Right-Pointing-Pointer NF{kappa}B-miR-155 pathway contributes to cell proliferation and resistance to cisplatin. -- Abstract: Recently, catecholamines have been described as being involved in the regulation of cancer genesis and progression. Here, we reported that adrenaline increased the cell proliferation and decreased the cisplatin induced apoptosis in HT29 cells. Further study found that adrenaline increased miR-155 expression in an NF{kappa}B dependent manner. HT29 cells overexpressing miR-155 had a higher cell growth rate and more resistance to cisplatin induced apoptosis. In contrast, HT29 cells overexpressing miR-155 inhibitor displayed decreased cell proliferation and sensitivity to cisplatin induced cell death. In summary, our study here revealed that adrenaline-NF{kappa}B-miR-155 pathway at least partially contributes to the psychological stress induced proliferation and chemoresistance in HT29 cells, shedding light on increasing the therapeutic strategies of cancer chemotherapy.

  15. ELECTRICAL ENGINEERING TECHNOLOGY PROGRAM EET 105: ELECTRICAL SYSTEMS

    E-Print Network [OSTI]

    Lozano-Nieto, Albert

    Storage -Energy can be stored in a capacitor to be released when desired Example: Defibrillator used1 ELECTRICAL ENGINEERING TECHNOLOGY PROGRAM EET 105: ELECTRICAL SYSTEMS Instructor: Albert Lozano. electrolytic - Non-electrolytic: smaller values: nF, pF - Electrolytic: Chemical process to increase

  16. The generating rank of the symplectic grassmannians

    E-Print Network [OSTI]

    Blok, Rieuwert J.

    The generating rank of the symplectic grassmannians: hyperbolic and isotropic geometry Rieuwert J.S.A. blokr@member.ams.org Accepted: March 7 2006 Key Words: symplectic geometry, grassmannian, generating to the symplectic group Sp2n(F) has generating rank 2n k - 2n k-2 when Char(F) = 2. #12;1 Introduction Generating

  17. Dynamics of the Dorsal morphogen gradient Jitendra S. Kanodiaa

    E-Print Network [OSTI]

    Shvartsman, Stanislav "Stas"

    Dynamics of the Dorsal morphogen gradient Jitendra S. Kanodiaa , Richa Rikhyb , Yoosik Kima Road, Princeton, NJ 08544; bCell Biology and Metabolism Branch, NIH, Building 32, 18 Library Drive localization gradient of Dorsal (Dl), a protein related to the mammalian NF- B transcription factors. Current

  18. Cache-Oblivious Red-Blue Line Segment Intersection

    E-Print Network [OSTI]

    Zeh, Norbert

    , and T is the number of intersections. 1 Introduction The memory systems of modern computers are becoming increasingly. Thus, it is important to design algorithms that are sensitive to the architecture of the memory systemNF-04-01- 0278, by an Ole Roemer Scholarship from the Danish National Science Research Council

  19. IOP PUBLISHING and INTERNATIONAL ATOMIC ENERGY AGENCY NUCLEAR FUSION Nucl. Fusion 48 (2008) 115008 (11pp) doi:10.1088/0029-5515/48/11/115008

    E-Print Network [OSTI]

    Harilal, S. S.

    2008-01-01

    IOP PUBLISHING and INTERNATIONAL ATOMIC ENERGY AGENCY NUCLEAR FUSION Nucl. Fusion 48 (2008) 115008 of Nuclear Engineering, Purdue University, 400 Central Drive, West Lafayette, IN 47907, USA E-mail: hassanein at stacks.iop.org/NF/48/115008 Abstract Safe and reliable operation is still one of the major challenges

  20. Synthesis and Characterization of Novel Fluorescent Injectable Micro-Carriers for Tissue Regeneration 

    E-Print Network [OSTI]

    Arora, Akshi

    2014-04-28

    monomer/initiator (M/I) molar ratios (20:1,100:1,200:1,400:1). The PLLA-EY polymer was characterized by FT-IR, UV-visible spectrophotometry and molecular weight (MW). The smooth walled (SW) and nanofibrous (NF) microspheres were fabricated from PLLA-EY 200...

  1. SHORT-TUBE SUBCRITICAL FLOW Enerag Division

    E-Print Network [OSTI]

    Oak Ridge National Laboratory

    due to the wide variation of the upstream and downstream conditions. In a previous paper [7 that at the same short tube upstream and downstream pressure differentials, the higher the level of upstream liquid and downstream pressure differentials as well as the level of upstream liquid subcooling. C = -0.019337 NF + 0

  2. Paper GT2004-54239 for 2004 Turbo Expo Austenitic Stainless Steels and Alloys With Improved High-Temperature

    E-Print Network [OSTI]

    Pennycook, Steve

    generation (DG), combined heat and power (CHP), and possibly combined cycle (microturbine-fuel cell recuperators/heat-exchangers increase the efficiency of both microturbines and smaller industrial gas turbines and corrosion resistance, including alloys NF709, HR120. Similar data are also provided on small lab heats

  3. Iodine Doped P3AT Structure 5.1 Introduction

    E-Print Network [OSTI]

    Winokur, Michael

    samples. The study of various intercalated structures by x­ray diffraction of polymer systems has a number doped P3AT films, the Osaka group has investigated a number of different dopants (NF \\Gamma 4 , BF \\Gamma 4 , AsF \\Gamma 6 , p­TS and Iodine) in P3ATs with side chain lengths ranging from butyl

  4. Spark-gap atomic emission microscopy. II. Improvements in resolution P. G. Van Patten,a)

    E-Print Network [OSTI]

    Myrick, Michael Lenn

    Spark-gap atomic emission microscopy. II. Improvements in resolution P. G. Van Patten,a) J. D. Noll which enhance performance in spark-gap atomic emission microscopy SGAEM experiments. SGAEM is a recent as small as 5 V, and intense atomic emission has been observed in such sparks. Small 1 nF , high

  5. Network Analyzer Basics This presentation covers the principles

    E-Print Network [OSTI]

    Anlage, Steven

    one-box) testers VSA Vector signal analyzer SA Spectrum analyzer VNA Vector signal analyzer TG/SA Tracking generator/spectrum analyzer SNA Scalar network analyzer NF Mtr. Noise-figure meter Imped. AnAgilent Network Analyzer Basics #12;2 Abstract This presentation covers the principles of measuring

  6. The impact of coexisting genetic mutations on murine optic glioma biology

    E-Print Network [OSTI]

    Kornfeld, S. Kerry

    the optic head relative to FMC and FMBC mice. Conclusions. Collectively, these experiments demonstrate be associated visual impairment5,6 or hypothalamic dysfunction.7 Since surgery and radiation therapy protein (neurofibromin) expression in these NF1-associated brain tumors.12,13 To model the human condition

  7. DELAY-DOPPLER CHANNEL ESTIMATION WITH ALMOST LINEAR COMPLEXITY --BY FISH, GUREVICH, HADANI, SAYEED, AND SCHWARTZ 1 Delay-Doppler Channel Estimation with Almost

    E-Print Network [OSTI]

    Sydney, University of

    . The vector space H is endowed with the inner product f1, f2 = nZN f1[n]f2[n], for f1, f2 H, and referred@math.utexas.edu. A. Sayeed is with the Department of Electrical and Computer Engineering, University of Wisconsin Engineering and Com- puter Science, University of California, Berkeley, CA 94720, USA. Email: odedsc

  8. Feature Selection in Automatic Music Genre Classification Carlos N. Silla Jr.

    E-Print Network [OSTI]

    Kent, University of

    Computing Laboratory Canterbury, CT2 7NF, Kent, United Kingdom cns2@kent.ac.uk Alessandro L. Koerich Pontifical Catholic University of Paran´a R. Imaculada Conceic¸~ao 1155, 80215-901, Curitiba, Brazil alekoe-901, Curitiba, Brazil celsokaestner@utfpr.edu.br Abstract This paper presents the results of the application

  9. A Machine Learning Approach to Automatic Music Genre

    E-Print Network [OSTI]

    Kent, University of

    NF Kent, United Kingdom cns2@kent.ac.uk 2 Pontifical Catholic University of Paraná R. Imaculada Conceição 1155, 80215-901 Curitiba - Paraná - Brazil alekoe@ppgia.pucpr.br 3 Federal University of Technology of Paraná Av. Sete de Setembro 3165, 80230-901 Curitiba - Paraná - Brazil celsokaestner

  10. Static-light hadrons on a dynamical anisotropic lattice

    E-Print Network [OSTI]

    Justin Foley; Alan O Cais; Mike Peardon; Sinead M. Ryan; Jon-Ivar Skullerud

    2005-11-02

    We present preliminary results for the static-light meson and baryon spectra for $N_f=2$ QCD. The study is performed on an anisotropic lattice and uses a new all-to-all propagator method allowing us to determine particle masses to a high precision.

  11. Indirect determination of zeta potential at high ionic strength: Specific application to semipermeable polymeric membranes

    E-Print Network [OSTI]

    reserved. 1. Introduction Membranes are rapidly becoming the leading separation method in a broad range and gas industry [6,12,29,30]. The fouling propensity of RO, NF, and FO membranes and mass transport to semipermeable polymeric membranes Bryan D. Coday a , Thomas Luxbacher b , Amy E. Childress c , Nohemi Almaraz

  12. Unfurling of the band 4.1, ezrin, radixin, moesin (FERM) domain of the merlin tumor suppressor

    SciTech Connect (OSTI)

    Yogesha, S.D.; Sharff, Andrew J.; Giovannini, Marco; Bricogne, Gerard; Izard, Tina (House Ear); (Globel Phasing); (Scripps)

    2014-10-02

    The merlin-1 tumor suppressor is encoded by the Neurofibromatosis-2 (Nf2) gene and loss-of-function Nf2 mutations lead to nervous system tumors in man and to several tumor types in mice. Merlin is an ERM (ezrin, radixin, moesin) family cytoskeletal protein that interacts with other ERM proteins and with components of cell-cell adherens junctions (AJs). Merlin stabilizes the links of AJs to the actin cytoskeleton. Thus, its loss destabilizes AJs, promoting cell migration and invasion, which in Nf2{sup +/-} mice leads to highly metastatic tumors. Paradoxically, the 'closed' conformation of merlin-1, where its N-terminal four-point-one, ezrin, radixin, moesin (FERM) domain binds to its C-terminal tail domain, directs its tumor suppressor functions. Here we report the crystal structure of the human merlin-1 head domain when crystallized in the presence of its tail domain. Remarkably, unlike other ERM head-tail interactions, this structure suggests that binding of the tail provokes dimerization and dynamic movement and unfurling of the F2 motif of the FERM domain. We conclude the 'closed' tumor suppressor conformer of merlin-1 is in fact an 'open' dimer whose functions are disabled by Nf2 mutations that disrupt this architecture.

  13. Prepared for the U.S. Department of Energy under Contract DE-AC02-76CH03073. Princeton Plasma Physics Laboratory

    E-Print Network [OSTI]

    .iop.org/NF/46/S933 Abstract The spectrum of compressional Alfv´en eigenmodes (CAE) is analysed and shown-D in which sub-cyclotron frequency instabilities of CAEs were observed at similar plasma conditions (W of these modes predicted by theory and observed in both devices. The discrete spectrum of CAEs is characterized

  14. Proceedings of the Fourth Linguistic Annotation Workshop, ACL 2010, pages 212216, Uppsala, Sweden, 15-16 July 2010. c 2010 Association for Computational Linguistics

    E-Print Network [OSTI]

    (153, 503, 508, '$phrase', hd, nx, []). By using the sentence number as the first argument of node/7 of abstraction over this representation. Direct dom- 212 #12;SIMPX VF NX PDS Dieser LK VXFIN VAFIN hat MF NX NX NN Auswirkungen PX APPR auf NX ART die NN Bereitschaft , NF S

  15. Assessment of four different cathode materials at different initial pHs using unbuffered catholytes in

    E-Print Network [OSTI]

    .12.037 #12;a solid anode can convert organic matter into CO2, releasing protons into solution and electrons Alkaline pH Nickel foam Molybdenum disulfide Stainless steel wool a b s t r a c t Nickel foam (NF

  16. Harold G. Kirk Brookhaven National Laboratory

    E-Print Network [OSTI]

    McDonald, Kirk

    Harold G. Kirk Brookhaven National Laboratory Future Targetry Plans NFMCC Collaboration Meeting Lawrence Berkeley National Laboratory January 26, 2009 #12;Harold G. Kirk Focus of Future Targetry Efforts Post-MERIT Magneto hydrodynamics IDS-NF #12;Harold G. Kirk MERIT Nozzle performance: The Issue #12

  17. Harold G. Kirk Brookhaven National Laboratory

    E-Print Network [OSTI]

    McDonald, Kirk

    Harold G. Kirk Brookhaven National Laboratory Target System Update IDS-NF Plenary Meeting Arlington, VA October 18, 2011 #12;Harold G. Kirk 2 Target Baseline: Proton Beam Assumptions Proton Beam Energy (rms) Beam * 30 cm Beam Power 4 MW (3.125 1015 protons/sec) #12;Harold G. Kirk 3 Target System

  18. Harold G. Kirk Brookhaven National Laboratory

    E-Print Network [OSTI]

    McDonald, Kirk

    Harold G. Kirk Brookhaven National Laboratory Target Baseline IDS-NF Plenary CERN March 23-24, 2009 #12;Harold G. Kirk The Neutrino Factory Target Concept #12;Harold G. Kirk Iron Plug Proton Beam Nozzle toward a Target System Design #12;Harold G. Kirk Alternative Collection System Another containment

  19. Nature GeNetics VOLUME 42 | NUMBER 11 | NOVEMBER 2010 991 Psoriasisisamultifactorialskindiseasecharacterizedby

    E-Print Network [OSTI]

    Abecasis, Goncalo

    Rel/NF-Btranscriptionfactorfamily. Psoriasis (MIM177900) is a chronic immune-mediated and hyperpro- liferative disorder of the skin factor for the development of psoriasis, implying that structural variants could also contribute screening (Supplementary Fig. 1). Our screening panel A Genome-wide association study identifies a psoriasis

  20. Reflective Cracking Study: First-level Report on Laboratory Shear Testing

    E-Print Network [OSTI]

    Tsai, Bor-Wen; Jones, David; Harvey, John T; Monismith, Carl L.; Guada, I.; Signore, J,

    2008-01-01

    2 and ? 2 ln? 3 and ? 3 lnG lnkcy5 lnn 1 lnn 2 lnNf lnpct559 Figure 4.28: Residual plots of LnG. (Pooled ShearResilient Shear Modulus, G* (lng) The Resilient Shear

  1. Abstract -A new analytical model for high-frequency noise in RF active CMOS mixers such as single-balanced and dou-

    E-Print Network [OSTI]

    Heydari, Payam

    Abstract - A new analytical model for high-frequency noise in RF active CMOS mixers such as single. The analytical model predicts that the output noise and NF are both a strong function of the LO frequency models for the mixer noise proposed in [1] and [2] are, therefore, incapable of accurately predicting

  2. ICE RUBBLE BUILD-UP ON CONICAL STRUCTURES DURING RIDGE INTERACTIONS

    E-Print Network [OSTI]

    Bruneau, Steve

    .E. Bruneau C-CORE, Memorial University of Newfoundland 51. John's, NF, CANADA, A1 B 3X5 ABSTRACT Ice tank. Comparisons were also made between the observed rubble build-up and calculations for limiting steady. The preferred designs for gravity base structures slope inward with increased elevation such that level ice

  3. APPLICATIONS OF MATHEMATICAL PROGRAMMING TECHNIQUES FOR

    E-Print Network [OSTI]

    Grossmann, Ignacio E.

    Gasses to Disposal Adsorption Column Wastewater Steam-Jet Ejector Mass Integration is used to lower mixed integer non linear programming (MINLP) problem. #12;DIRECT RECYCLE CONFIGURATION #12;j=1, G1 j=2 p NPROP SOURCES i=1 Process Wi i=2 i=NS Fresh Fr r=1 r=NF Direct Recycle Property

  4. (BBA) is competent for investigations on incidents of poisoning honey bees due to the use of pesti-

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    (BBA) is competent for investigations on incidents of poisoning honey bees due to the use of pesti in the case of honey-bee poisoning by pesticides' [Amtl Pfl Best (Berlin-Dahlem), NF 36/5, 179-182]. An overview of the poisoning incidents shows a clear decline in the last years. The most impor- tant

  5. Abstract--In this paper, we explain that the human knee behavior in the weight acceptance phase of gait (first ~40% of

    E-Print Network [OSTI]

    Haller, Gary L.

    exoskeletons [1, 13]. These observations led researchers to conduct basic research on the human physiology in interaction with exoskeletal systems [7, 9, 12, 14]. In order to expand upon this line of basic research, we was supported by the US Army Natick Soldier Research Development and Engineering Center, contract #W911NF-07-D

  6. Computer Science at Kent On Modular Termination Proofs

    E-Print Network [OSTI]

    Kent, University of

    Computer Science at Kent On Modular Termination Proofs Jonathan C. Martin and Andy King Technical by the Computing Laboratory, University of Kent, Canterbury, Kent CT2 7NF, UK. Abstract Reasoning about termination is a key issue in logic program development. One classic technique for proving termination is to construct

  7. IEEE TRANSACTIONS ON SYSTEMS, MAN, AND CYBERNETICS--PART B: CYBERNETICS, VOL. 39, NO. 6, DECEMBER 2009 1393 Cooperative Control and Potential Games

    E-Print Network [OSTI]

    Arslan, Gürdal

    IEEE TRANSACTIONS ON SYSTEMS, MAN, AND CYBERNETICS--PART B: CYBERNETICS, VOL. 39, NO. 6, DECEMBER, California Institute of Technology, by the Army Research Office under Grant W911NF04316, by the Air Force. R. Marden is with the Social and Information Sciences Laboratory, California Institute of Technology

  8. Ecology, biology, and coexistence of four species of Aradidae from the southeastern United States 

    E-Print Network [OSTI]

    Taylor, Steven Joseph

    1987-01-01

    Aradus acutus N 1 N 1 t Quercus ~ni ra (water oak) Quercus ~ni ra (water oak) Quercus rubra or Quercus falcata (red oak) p~tp~t cabbage palmetto N *dt. * * ~1* Q ~nf ( *t N) Quercus ruhr a (northern red oak) Quercus velutina (black oak) p~t* Fa...

  9. SecureSwitch: BIOS-Assisted Isolation and Switch between Trusted and Untrusted Commodity OSes

    E-Print Network [OSTI]

    Stavrou, Angelos

    was supported in part by the U.S. Army Research Of- fice (ARO) through contract WF911NF-11-C-0048, the U.S. Air modifications. Our aim is to eliminate any mutable, non-BIOS code sharing while se- curely reusing existing Force Research Laboratory (AFRL) through contract FA8650-10-C-7024, NSF grant CNS-TC 0915291

  10. 5626 JOURNAL OF LIGHTWAVE TECHNOLOGY, VOL. 27, NO. 24, DECEMBER 15, 2009 Thermal and Mechanical Analysis of Cross-Linked

    E-Print Network [OSTI]

    Matthewson, M. John

    on an acrylate-based polymer system due to its relatively low cost and its ability to be cured at high speed on; current version published November 13, 2009. This work was supported in part by the Joint Technology Office (JTO) of the US Department of Defense (DoD) under MRI Contract Number W911NF-05-1-0517 to Clemson

  11. 390 IEEE JOURNAL OF QUANTUM ELECTRONICS, VOL. 41, NO. 3, MARCH 2005 MEMS-Tunable Vertical-Cavity SOAs

    E-Print Network [OSTI]

    MacDonald, Noel C.

    390 IEEE JOURNAL OF QUANTUM ELECTRONICS, VOL. 41, NO. 3, MARCH 2005 MEMS-Tunable Vertical properties, and noise figure (NF) of MEMS-based widely tunable vertical-cavity semiconductor optical electrostatic actuator are outlined. It is found that MEMS-tunable VCSOAs follow many of the same design trends

  12. A PITCH EXTRACTION ALGORITHM TUNED FOR AUTOMATIC SPEECH RECOGNITION Pegah Ghahremani1

    E-Print Network [OSTI]

    Povey, Dan

    partially supported by DARPA BOLT contract No ¯ HR0011-12-C-0015 and are affiliated with the Human Language Khudanpur was partially supported by IARPA BABEL contract No ¯W911NF-12-C-0015. Povey and Khudanpur were Hopkins University, where he received partial support from Cisco Research Award CG#574560 and from a gift

  13. PARAMETRIC EXCITATION OF PLASMA OSCILLATIONS IN JOSEPHSON JUNCTIONS

    E-Print Network [OSTI]

    Boyer, Edmond

    b. ' FIG. 3. - (a) 03B1c tan çoo vs. 2/Q at co = 2 cop. Straight line : theory. Circles : analog harmonic generation with big amplitude may be understood from a discussion of the stability properties frequency FIG. 1. - The junction model. For the analog R = 500 il, Io = 1 mA, C = 100 nF, and k = « 2 e

  14. Managed by UT-Battelle for the Department of Energy

    E-Print Network [OSTI]

    McDonald, Kirk

    Managed by UT-Battelle for the Department of Energy Design and Operational Features of a Mercury CERN #12;2 Managed by UT-Battelle for the Department of Energy Contributors · Tom McManamy, Chief Dec 08 #12;3 Managed by UT-Battelle for the Department of Energy NF-IDS Meeting 15 Dec 08 Outline

  15. Managed by UT-Battelle for the Department of Energy

    E-Print Network [OSTI]

    McDonald, Kirk

    Managed by UT-Battelle for the Department of Energy Neutrino Factory Mercury Containment Concepts V;2 Managed by UT-Battelle for the Department of Energy HPT Workshop, 6 Nov 2008 Outline · Neutrino Factory.14 Dimensions in cm #12;6 Managed by UT-Battelle for the Department of Energy HPT Workshop, 6 Nov 2008 NF Hg Jet

  16. Chicane shielding and energy deposition (IPAC'13 followup)

    E-Print Network [OSTI]

    McDonald, Kirk

    Chicane shielding and energy deposition (IPAC'13 followup) Pavel Snopok IDSNF phone meetingIDS NF. Radius = 4353 cm, length of 18 cm, onaxis field 1.5 T throughout the channel. · Cyan: W shielding (pure W need to change the density to 60%) 4 cm thickness @· Cyan: W shielding (pure W, need to change

  17. 1. (25 pts) Three point charges, q1, q2 and q3 that have the same +2 Coulomb charge are fixed to a circle of radius

    E-Print Network [OSTI]

    Akerib, Daniel S.

    of 6.0 nF and a plate area of 2 2 5.0 10 m- × is connected to a 12 V battery. a. What is the electric A wire and the magnetic field at point O is zero tesla. a. Determine the value of the current, I

  18. Radiation-induced gene responses

    SciTech Connect (OSTI)

    Woloschak, G.E.; Paunesku, T.; Shearin-Jones, P.; Oryhon, J.

    1996-12-31

    In the process of identifying genes that are differentially regulated in cells exposed to ultraviolet radiation (UV), we identified a transcript that was repressed following the exposure of cells to a combination of UV and salicylate, a known inhibitor of NF-kappaB. Sequencing this band determined that it has identify to lactate dehydrogenase, and Northern blots confirmed the initial expression pattern. Analysis of the sequence of the LDH 5` region established the presence of NF-kappaB, Sp1, and two Ap-2 elements; two partial AP- 1; one partial RE, and two halves of E-UV elements were also found. Electromobility shift assays were then performed for the AP-1, NF- kappaB, and E-UV elements. These experiments revealed that binding to NF-kappaB was induced by UV but repressed with salicylic acid; UV did not affect AP-1 binding, but salicylic acid inhibited it alone or following UV exposure; and E-UV binding was repressed by UV, and salicylic acid had little effect. Since the binding of no single element correlated with the expression pattern of LDH, it is likely that multiple elements govern UV/salicylate-mediated expression.

  19. TECTONOPHYSICS Waves of the future: Superior inferences from collocated seismic

    E-Print Network [OSTI]

    Jones, Alan G.

    . processing. ll111delling and inversion of particularly natural-source magnetotelluric (\\1TJ data in modelling and inversion. Also. laboratory measuremeilh of seismic. electrical and rheological properties nf a Southern BI itish Columhia California Alherta Ontano QueheL' and Ontario \\Vc....t ('oa~t Ncw Mexico Alilc

  20. Timed CSP and Object-Z John Derrick

    E-Print Network [OSTI]

    Kent, University of

    Timed CSP and Object-Z John Derrick Computing Laboratory, University of Kent, Canterbury, CT2 7NF a simple integration of timed CSP and Object-Z. Following existing work, the components in such an inte- gration are written as either Object-Z classes, or timed CSP processes, and are combined together using

  1. Quantitative Objectives Report CohoReport

    E-Print Network [OSTI]

    (%) Lower Columbia River Coho Cascade Toutle NF Early-run (Type- S)and Late- run (Type-N) 1900 Primary High 180ThreatenedLower Columbia River Willamette Lower Columbia Washougal Late-run (Type- N) 1500 Cowlitz Late-run (Type- N) 2000 Primary (1) High (1) >500ThreatenedLower Columbia River Willamette Lower

  2. Vol. 46 No. 7 pJs=hM3X2qO@J8;o July 2005 J,;6%=%U%H%&%'%"$N%F%9%H$KE,$7$?

    E-Print Network [OSTI]

    Chiba, Shigeru

    !y C1BN%F%9%H$H$O!$695A$K$O!$%=%U%H%&%'%"$r9=C[$9$k%b%8%e!¡ %k$=$l$¿$l$NF0:n3NG'%A%'%C%/$r0UL#$9$k

  3. Communicating oscillatory networks: Frequency Domain Analysis

    E-Print Network [OSTI]

    Ihekwaba, Adaoha E C; Sedwards, Sean

    2011-12-22

    -based systematic analysis technique that attempts to address the challenge of network assembly by defining a rigorous means to quantify the behaviour of stochastic systems. As our focus we construct a novel coupled oscillatory model of p53, NF-kB and the mammalian...

  4. Pseudomonas syringae pv. actinidiae (PSA) Isolates from Recent Bacterial Canker of Kiwifruit Outbreaks Belong to

    E-Print Network [OSTI]

    Guttman, David S.

    Pseudomonas syringae pv. actinidiae (PSA) Isolates from Recent Bacterial Canker of Kiwifruit fruit (Actinidia deliciosa and A. chinensis) caused by Pseudomonas syringae pv. actinidiae (PSA, Taratufolo MC, Cai R, Almeida NF, et al. (2012) Pseudomonas syringae pv. actinidiae (PSA) Isolates from

  5. Ni(OH){sub 2} nanoflakes electrodeposited on Ni foam-supported vertically oriented graphene nanosheets for application in asymmetric supercapacitors

    SciTech Connect (OSTI)

    Wang, Xin; Liu, Jiyue; Wang, Yayu; Zhao, Cuimei; Zheng, Weitao

    2014-04-01

    Highlights: • Ni(OH){sub 2}/vertically oriented graphene nanosheets (V-GNs) was prepared. • Ni(OH){sub 2}/V-GNs had enhanced specific capacitance, cycling reversibility and stability. • Performance of Ni(OH){sub 2}/GNs/NF-AC asymmetric supercapacitor was studied. - Abstract: Binderless Ni(OH){sub 2} nanoflakes grown on Ni foam (NF)-supported vertically oriented graphene nanosheets (V-GNs) has been fabricated as a positive electrode material for asymmetric supercapacitor (ASC), coupled with activated carbon (AC) as a counter electrode material. The introduction of V-GNs leads to dense growth of nanocrystalline ?-Ni(OH){sub 2} that is confirmed by X-ray diffraction, transmission electron microscopic and scanning electron microscopic analyses. The electrochemical performances of the Ni(OH){sub 2}/GNs/NF electrode are characterized by cyclic voltammetry and charge–discharge tests, which exhibit high specific capacitance of 2215 F g{sup ?1} at a scan current density of 2.3 A g{sup ?1}, enhanced cycling stability and high rate capability. The Ni(OH){sub 2}/GNs/NF-AC-based ASC can achieve a cell voltage of 1.4 V and a specific energy density of 11.11 Wh kg{sup ?1} at 0.5 mA cm{sup ?2} with a nearly 100% coulombic efficiency at room temperature.

  6. Sum Rules and Cutoff Effects in Wilson Lattice QCD

    E-Print Network [OSTI]

    Harvey B. Meyer

    2006-09-06

    We use the transfer matrix formalism to derive non-perturbative sum rules in Wilson's lattice QCD with N_f flavours of quarks. The discretization errors on these identities are treated in detail. As an application, it is shown how the sum rules can be exploited to give improved estimates of the continuum spectrum and static potential.

  7. Asymptotic scaling corrections in QCD with Wilson fermions from the 3-loop average plaquette

    E-Print Network [OSTI]

    B. Alles; A. Feo; H. Panagopoulos

    1998-01-23

    We calculate the 3-loop perturbative expansion of the average plaquette in lattice QCD with N_f massive Wilson fermions and gauge group SU(N). The corrections to asymptotic scaling in the corresponding energy scheme are also evaluated. We have also improved the accuracy of the already known pure gluonic results at 2 and 3 loops.

  8. SIRT1 inactivation induces inflammation through the dysregulation of autophagy in human THP-1 cells

    SciTech Connect (OSTI)

    Takeda-Watanabe, Ai; Kitada, Munehiro; Kanasaki, Keizo; Koya, Daisuke

    2012-10-12

    Highlights: Black-Right-Pointing-Pointer SIRT1 inactivation decreases autophagy in THP-1 cell. Black-Right-Pointing-Pointer Inhibition of autophagy induces inflammation. Black-Right-Pointing-Pointer SIRT1 inactivation induces inflammation through NF-{kappa}B activation. Black-Right-Pointing-Pointer The p62/Sqstm1 accumulation by impairment of autophagy is related to NF-{kappa}B activation. Black-Right-Pointing-Pointer SIRT1 inactivation is involved in the activation of mTOR and decreased AMPK activation. -- Abstract: Inflammation plays a crucial role in atherosclerosis. Monocytes/macrophages are some of the cells involved in the inflammatory process in atherogenesis. Autophagy exerts a protective effect against cellular stresses like inflammation, and it is regulated by nutrient-sensing pathways. The nutrient-sensing pathway includes SIRT1, a NAD{sup +}-dependent histone deacetylase, which is implicated in the regulation of a variety of cellular processes including inflammation and autophagy. The mechanism through which the dysfunction of SIRT1 contributes to the regulation of inflammation in relation to autophagy in monocytes/macrophages is unclear. In the present study, we demonstrate that treatment with 2-[(2-Hydroxynaphthalen-1-ylmethylene)amino]-N-(1-phenethyl)benzamide (Sirtinol), a chemical inhibitor of SIRT1, induces the overexpression of inflammation-related genes such as tumor necrosis factor (TNF)-{alpha} and interleukin (IL)-6 through nuclear factor (NF)-{kappa}B signaling activation, which is associated with autophagy dysfunction, as shown through p62/Sqstm1 accumulation and decreased expression of light chain (LC) 3 II in THP-1 cells. The autophagy inhibitor, 3-methyladenine, also induces inflammation-related NF-{kappa}B activation. In p62/Sqstm1 knockdown cells, Sirtinol-induced inflammation through NF-{kappa}B activation is blocked. In addition, inhibition of SIRT1 is involved in the activation of the mammalian target of rapamycin (mTOR) pathway and is implicated in decreased 5 Prime -AMP activated kinase (AMPK) activation, leading to the impairment of autophagy. The mTOR inhibitor, rapamycin, abolishes Sirtinol-induced inflammation and NF-{kappa}B activation associated with p62/Sqstm1 accumulation. In summary, SIRT1 inactivation induces inflammation through NF-{kappa}B activation and dysregulates autophagy via nutrient-sensing pathways such as the mTOR and AMPK pathways, in THP-1 cells.

  9. Arsenic augments the uptake of oxidized LDL by upregulating the expression of lectin-like oxidized LDL receptor in mouse aortic endothelial cells

    SciTech Connect (OSTI)

    Hossain, Ekhtear; Ota, Akinobu; Karnan, Sivasundaram; Damdindorj, Lkhagvasuren; Takahashi, Miyuki; Konishi, Yuko; Konishi, Hiroyuki; Hosokawa, Yoshitaka

    2013-12-15

    Although chronic arsenic exposure is a well-known risk factor for cardiovascular diseases, including atherosclerosis, the molecular mechanism underlying arsenic-induced atherosclerosis remains obscure. Therefore, this study aimed to elucidate this molecular mechanism. We examined changes in the mRNA level of the lectin-like oxidized LDL (oxLDL) receptor (LOX-1) in a mouse aortic endothelial cell line, END-D, after sodium arsenite (SA) treatment. SA treatment significantly upregulated LOX-1 mRNA expression; this finding was also verified at the protein expression level. Flow cytometry and fluorescence microscopy analyses showed that the cellular uptake of fluorescence (Dil)-labeled oxLDL was significantly augmented with SA treatment. In addition, an anti-LOX-1 antibody completely abrogated the augmented uptake of Dil-oxLDL. We observed that SA increased the levels of the phosphorylated forms of nuclear factor of kappa light polypeptide gene enhancer in B cells (NF-?B)/p65. SA-induced upregulation of LOX-1 protein expression was clearly prevented by treatment with an antioxidant, N-acetylcysteine (NAC), or an NF-?B inhibitor, caffeic acid phenethylester (CAPE). Furthermore, SA-augmented uptake of Dil-oxLDL was also prevented by treatment with NAC or CAPE. Taken together, our results indicate that arsenic upregulates LOX-1 expression through the reactive oxygen species-mediated NF-?B signaling pathway, followed by augmented cellular oxLDL uptake, thus highlighting a critical role of the aberrant LOX-1 signaling pathway in the pathogenesis of arsenic-induced atherosclerosis. - Highlights: • Sodium arsenite (SA) increases LOX-1 expression in mouse aortic endothelial cells. • SA enhances cellular uptake of oxidized LDL in dose-dependent manner. • SA-induced ROS generation enhances phosphorylation of NF-?B. • SA upregulates LOX-1 expression through ROS-activated NF-?B signaling pathway.

  10. Nicotine stimulates urokinase-type plasminogen activator receptor expression and cell invasiveness through mitogen-activated protein kinase and reactive oxygen species signaling in ECV304 endothelial cells

    SciTech Connect (OSTI)

    Khoi, Pham Ngoc; Park, Jung Sun; Kim, Nam Ho; Jung, Young Do, E-mail: ydjung@chonnam.ac.kr

    2012-03-01

    Urokinase-type plasminogen activator receptor (uPAR) expression is elevated during inflammation, tissue remodeling and in many human cancers. This study investigated the effect of nicotine, a major alkaloid in tobacco, on uPAR expression and cell invasiveness in ECV304 endothelial cells. Nicotine stimulated uPAR expression in a dose-dependent manner and activated extracellular signal-regulated kinases-1/2 (Erk-1/2), c-Jun amino-terminal kinase (JNK) and p38 mitogen activated protein kinase (MAPK). Specific inhibitors of MEK-1 (PD98059) and JNK (SP600125) inhibited the nicotine-induced uPAR expression, while the p38 MAPK inhibitor SB203580 did not. Expression vectors encoding dominant negative MEK-1 (pMCL-K97M) and JNK (TAM67) also prevented nicotine-induced uPAR promoter activity. The intracellular hydrogen peroxide (H{sub 2}O{sub 2}) content was increased by nicotine treatment. The antioxidant N-acetylcysteine prevented nicotine-activated production of reactive oxygen species (ROS) and uPAR expression. Furthermore, exogenous H{sub 2}O{sub 2} increased uPAR mRNA expression. Deleted and site-directed mutagenesis demonstrated the involvement of the binding sites of transcription factor nuclear factor-kappaB (NF-?B) and activator protein (AP)-1 in the nicotine-induced uPAR expression. Studies with expression vectors encoding mutated NF-?B signaling molecules and AP-1 decoy confirmed that NF-?B and AP-1 were essential for the nicotine-stimulated uPAR expression. MAPK (Erk-1/2 and JNK) and ROS functioned as upstream signaling molecules in the activation of AP-1 and NF-?B, respectively. In addition, ECV304 endothelial cells treated with nicotine displayed markedly enhanced invasiveness, which was partially abrogated by uPAR neutralizing antibodies. The data indicate that nicotine induces uPAR expression via the MAPK/AP-1 and ROS/NF-?B signaling pathways and, in turn, stimulates invasiveness in human ECV304 endothelial cells. -- Highlights: ? Endothelial cells treated with nicotine displayed enhanced invasiveness. ? Nicotine induces uPAR expression and, in turn, stimulates invasiveness. ? MAPK/AP-1 and ROS/NF-?B signals are involved in nicotine-induced uPAR.

  11. Waves and vortices in the inverse cascade regime of stratified turbulence with or without rotation

    E-Print Network [OSTI]

    Herbert, Corentin; Rosenberg, Duane; Pouquet, Annick

    2015-01-01

    We study the partition of energy between waves and vortices in stratified turbulence, with or without rotation, for a variety of parameters, focusing on the behavior of the waves and vortices in the inverse cascade of energy towards the large scales. To this end, we use direct numerical simulations in a cubic box at a Reynolds number Re=1000, with the ratio between the Brunt-V\\"ais\\"al\\"a frequency N and the inertial frequency f varying from 1/4 to 20, together with a purely stratified run. The Froude number, measuring the strength of the stratification, varies within the range 0.02 energy spectra and fluxes exhibit characteristics of an inverse cascade, even though their energy is not conserved. Surprisingly, the slow vortices still dominate when the ratio N/f increases, also in the stratified case, although less and less so. However, when N/f increases, the inverse cascade of the slow modes becomes we...

  12. Measurement of the EMC effect in the deuteron

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Griffioen, Keith A.; Arrington, John R.; Christy, M. Eric; Ent, Rolf; Kalantarians, Narbe; Keppel, Cynthia E.; Kuhn, Sebastian E.; Melnitchouk, Wally; Niculescu, Gabriel; Niculescu, Ioana; et al

    2015-07-01

    We have determined the structure function ratio $R^d_{\\rm EMC}=F_2^d/(F_2^n+F_2^p)$ from recently published $F_2^n/F_2^d$ data taken by the BONuS experiment using CLAS at Jefferson Lab. This ratio deviates from unity, with a slope $dR_{\\rm EMC}^{d}/dx= -0.10\\pm 0.05$ in the range of Bjorken $x$ from 0.35 to 0.7, for invariant mass $W>1.4$ GeV and $Q^2>1$ GeV$^2$. The observed EMC effect for these kinematics is consistent with conventional nuclear physics models that include off-shell corrections, as well as with empirical analyses that find the EMC effect proportional to the probability of short-range nucleon-nucleon correlations.

  13. Mott multicriticality of Dirac electrons in graphene

    E-Print Network [OSTI]

    Classen, Laura; Janssen, Lukas; Scherer, Michael M

    2015-01-01

    We study the multicritical behavior for the semimetal-insulator transitions on graphene's honeycomb lattice using the Gross-Neveu-Yukawa effective theory with two order parameters: the SO(3) (Heisenberg) order parameter describes the antiferromagnetic transition, and the $\\mathbb{Z}_2$ (Ising) order parameter describes the transition to a staggered density state. Their coupling induces multicritical behavior which determines the structure of the phase diagram close to the multicritical point. Depending on the number of fermion flavors $N_f$ and working in the perturbative regime in vicinity of three (spatial) dimensions, we observe first order or continuous phase transitions at the multicritical point. For the graphene case of $N_f=2$ and within our low order approximation, the phase diagram displays a tetracritical structure.

  14. Quantum Critical Behaviour in a Graphene-like Model

    E-Print Network [OSTI]

    Simon Hands; Costas Strouthos

    2008-06-30

    We present the first results of numerical simulations of a 2+1 dimensional fermion field theory based on a recent proposal for a model of graphene, consisting of N_f four-component Dirac fermions moving in the plane and interacting via an instantaneous Coulomb interaction. In the strong-coupling limit we identify a critical number of flavors N_fc=4.8(2) separating an insulating from a conducting phase. This transition corresponds to the location of a quantum critical point, and we use a fit to the equation of state for the chiral order parameter to estimate the critical exponents. Next we simulate N_f=2 corresponding to real graphene, and approximately locate a transition from strong to weak coupling behaviour. Strong correlations are evident in the weak-coupling regime.

  15. Quantum Phase Transition in a Graphene Model

    E-Print Network [OSTI]

    Simon Hands; Costas Strouthos

    2008-08-20

    We present results for the equation of state of a graphene-like model in an effort to understand the properties of its quantum phase transition. The N_f fermion species interact through a three dimensional instantaneous Coulomb potential. Since there are no reliable analytical tools that work for all values of N_f and the coupling constant g, we rely on Monte Carlo simulations to calculate the critical properties of the model near the phase transition. We consider the four-component formulation for the fermion fields, which arises naturally as the continuum limit of the staggered fermion construction in (2+1) dimensions. In the limit of infinitely strong Coulomb interaction, the system undergoes a quantum phase transition at a critical number of fermion species N_fc ~ 4.7. We also calculate the values of the critical exponents at the quantum phase transition.

  16. Properties of Some Integral Transforms Arising in Tomography 

    E-Print Network [OSTI]

    Moon, Sunghwan

    2013-10-11

    , the inverse Fourier transform is f(x) = F?1fˆ(x) := 1 (2pi)n ? Rn fˆ(?)eix·?d?. The Plancherel theorem claims that for f, g ? L1(Rn) ? L2(Rn), one has ? Rn fˆ(?)gˆ(?)d? = 2pi ? Rn f(x)g(x)dx. (1.1) 2 The Fourier transform extends to a bijection from L2(Rn... function f with compact support in Bn, c(?, n)||f ||? ? ||Rf ||?+(n?1)/2 ? C(?, n)||f ||?, (1.4) 4 where ||f ||2? = ? Rn (1 + |?|2)?|fˆ(?)|2d? and ||g||2? = ? Sn?1 ? R (1 + ?2)?|gˆ(?, ?)|2d?d?, where gˆ is the Fourier transform of a function g on Sn?1 × R...

  17. Pancreatic cancer: Pathogenesis, prevention and treatment

    SciTech Connect (OSTI)

    Sarkar, Fazlul H. Banerjee, Sanjeev; Li, Yiwei

    2007-11-01

    Pancreatic cancer is the fourth leading cause of cancer death in the United States with a very low survival rate of 5 years. To better design new preventive and/or therapeutic strategies for the fight against pancreatic cancer, the knowledge of the pathogenesis of pancreatic cancer at the molecular level is very important. It has been known that the development and the progression of pancreatic cancer are caused by the activation of oncogenes, the inactivation of tumor suppressor genes, and the deregulation of many signaling pathways among which the EGFR, Akt, and NF-{kappa}B pathways appear to be most relevant. Therefore, the strategies targeting EGFR, Akt, NF-{kappa}B, and their downstream signaling could be promising for the prevention and/or treatment of pancreatic cancer. In this brief review, we will summarize the current knowledge regarding the pathogenesis, prevention, and treatment of pancreatic cancer.

  18. The DIS(chi) Scheme for Heavy Quark Production at Small x.

    E-Print Network [OSTI]

    White, C D

    (nf ) g(nf ) ) , (1) 1 February 2, 2008 6:9 Proceedings Trim Size: 9in x 6in Cavendish-HEP-2006/12 2 which defines the heavy matrix elements {Aij}. This leads to an ambiguity in the VF coefficient functions. For example, at O(?S), one finds: CV F (0)2,H ?A... . The resummed prediction is compared with data alongside a standard NLO prediction from a global fit in figure 1. The resummed 0.5 1 1.5 2 2.5 3 1 10 H1 (×1.02) ZEUS (×1.004) x=5×10-5 x=6.3×10-5 x=8×10-5 x=1.3×10-4 x=1.61×10-4 x=2×10-4 x=2.53×10-4 x=3.2×10-4 x=4...

  19. Football - Individuals - 1930s - 1 

    E-Print Network [OSTI]

    Unknown

    2005-09-05

    the design of efficient input circuits for c u s s c amplifiers t heddsigngeof cpi utramh lX jr?cla? t??io?s? nd go dg??s nf? ?ofgsfg ??e usn? o? ??s?gie?n? ?f?efssief? hs?nig ?sfg theds igno lu? h?c??? fc ?jj?????l ????l ??r???lc jar ??cc... ? t??m?j??rc utramh lX jr?cla? ?e t heddsigngeof c???eggs? go g?s ?in??ngs c??oo? o? g?s t?ie???g?in? nf? ?s??nfe?n? ?o??s?s o? ls?nd ef ?nigen? ????e???sfg o? g?s is??eis?sfgd ?oi g?s ?s?iss pu ha?lar aj ?u?maca?u? theds igno rampl Xhjmd...

  20. :Epigallocatechin-3-Gallate, a histone acetyltransferase inhibitor, inhibits EBV-induced B Lymphocytr transformation via suppression

    E-Print Network [OSTI]

    Huang, Ching-Tsan

    expression in respiratory epithelium. Inflammation, 2002. 26(5): p. 233-41. 3. Syed, D.N., et al., Green tea.8 1. Chen, L.F. and W.C. Greene, Regulation of distinct biological activities of the NF-kappaB transcription factor complex by acetylation. J Mol Med, 2003. 81(9): p. 549-57. 2. Chen, P.C., et al., A green

  1. Product specification Supersedes data of 30th November 2000

    E-Print Network [OSTI]

    Berns, Hans-Gerd

    in shaded cells indicate thickness class. Thickness classification and packing quantities C (nF) LAST TWO DIGITS OF 12NC 16 V 25 V 0402 0603 0805 1206 0402 0603 0805 1206 1210 3.3 29 0.5 ±0.05 4.7 32 6.8 34 10 61 1.15 ±0.1 1000 63 THICKNESS CLASSIFICATION (mm) 8 mm TAPE WIDTH QUANTITY PER REEL QUANTITY PER

  2. EIS-0350-S1: Supplemental Environmental Impact Statement for the Nuclear Facility Portion of the Chemistry and Metallurgy Research Building Replacement Project at Los Alamos National Laboratory, New Mexico

    Broader source: Energy.gov [DOE]

    This Supplemental EIS evaluates the completion of the Chemistry and Metallurgy Research Building Replacement (CMRR) Project, which consists of constructing the nuclear facility portion (CMRR-NF) at Los Alamos National Laboratory (LANL). The CMRR Project provides the analytical chemistry and materials characterization capabilities currently or previously performed in the existing Chemistry and Metallurgy Research (CMR) Building. Because of recent detailed site geotechnical investigations, certain aspects of the CMRR-NR project have changed resulting in change to the environmental impacts.

  3. SOME ANALYTIC MODELS OF PASSIVE SOLAR BUILDING PERFORMANCE: A THEORETICAL APPROACH TO THE DESIGN OF ENERGY-CONSERVING BUILDINGS

    E-Print Network [OSTI]

    Goldstein, David Baird

    2011-01-01

    N f Cf A A P A w A U wo C w Ufo Cf A G A f A p U NfNw q ~= CA A = A f G (1 _ N f ) N R Ufo C f A F = A R N N N f R A Q == A f A N h f A (a s) R N A Ufo a = A f - + C - N f R Table

  4. NUMERICAL ANALYSIS OF FLOW FIELDS GENERATED BY ACCELERATING FLAMES

    E-Print Network [OSTI]

    Kurylo, J.

    2011-01-01

    TR=D60*1 pf'o/nf>o/I GF-I '+UfO*.2/0f C. *2/2. ' C-----A(CTC SC=INITIAL STEP ~IZE C A"UFO=SPtC VOL "ATIC FCI' CCNST PRE=CFI". ~1 $ UPCCl,I,::UFO',O) EPC(I,I'=EP(M,O' $ PPC(I, II=

  5. Hybrid r-Vacua in N=2 Supersymmetric QCD: Universal Condensate Formula

    E-Print Network [OSTI]

    M. Shifman; A. Yung

    2013-03-06

    We derive an exact unified formula for all condensates (quark and monopole) in the hybrid r vacua in N=2 supersymmetric QCD slightly deformed by a \\mu A^2 term. The gauge group is assumed to be U(N) and the number of the quark flavors N_f subject to the condition Nhybrid r vacua in \\mu-deformed N=2 SQCD (the small-\\mu limit).

  6. Cytokine secretion profiles in primary cultured cells and in mice stimulated with plasmid DNA

    E-Print Network [OSTI]

    Yoshida, Hiroyuki

    2006-10-27

    University Elimination of DNA by antigen presenting cells Gene therapy Infection Leakage Processing Macrophages and dendritic cells Apoptotic or necrotic cells Endogenous DNA DNA Virus Plasmid DNA Exogenous DNA CpG motif5?- Pur Pur CG Pyr Pyr -3? Danger... signal Naked CpG DNA DNA lipoplex Macrophages or dendritic cells ???? ???? Cytokine geneNF-?B AP-1 ???? ???? Endosome Nucleus ? TLR9 Interferon ?/? gene Inflammatory cytokines TNF-?, IL-6 etc. Type I interferon IFN-?, INF-? ? Mechanisms of immune response...

  7. Assessment of energetic costs of AhR activation by ?-naphthoflavone in rainbow trout (Oncorhynchus mykiss) hepatocytes using metabolic flux analysis

    SciTech Connect (OSTI)

    Nault, Rance; Abdul-Fattah, Hiba; Mironov, Gleb G.; Berezovski, Maxim V.; Moon, Thomas W.

    2013-08-15

    Exposure to environmental contaminants such as activators of the aryl hydrocarbon receptor (AhR) leads to the induction of defense and detoxification mechanisms. While these mechanisms allow organisms to metabolize and excrete at least some of these environmental contaminants, it has been proposed that these mechanisms lead to significant energetic challenges. This study tests the hypothesis that activation of the AhR by the model agonist ?-naphthoflavone (?NF) results in increased energetic costs in rainbow trout (Oncorhynchus mykiss) hepatocytes. To address this hypothesis, we employed traditional biochemical approaches to examine energy allocation and metabolism including the adenylate energy charge (AEC), protein synthesis rates, Na{sup +}/K{sup +}-ATPase activity, and enzyme activities. Moreover, we have used for the first time in a fish cell preparation, metabolic flux analysis (MFA) an in silico approach for the estimation of intracellular metabolic fluxes. Exposure of trout hepatocytes to 1 ?M ?NF for 48 h did not alter hepatocyte AEC, protein synthesis, or Na{sup +}/K{sup +}-ATPase activity but did lead to sparing of glycogen reserves and changes in activities of alanine aminotransferase and citrate synthase suggesting altered metabolism. Conversely, MFA did not identify altered metabolic fluxes, although we do show that the dynamic metabolism of isolated trout hepatocytes poses a significant challenge for this type of approach which should be considered in future studies. - Highlights: • Energetic costs of AhR activation by ?NF was examined in rainbow trout hepatocytes. • Metabolic flux analysis was performed on a fish cell preparation for the first time. • Exposure to ?NF led to sparing of glycogen reserves and altered enzyme activities. • Adenylate energy charge was maintained despite temporal changes in metabolism.

  8. Unity, Justice and Protection: The Colored Trainmen of America's Struggle to End Jim Crow in the American Railroad Industry [and Elsewhere] 

    E-Print Network [OSTI]

    James, Ervin

    2012-10-19

    to monumental federal court cases such as Smith v. Allwright (1944), Sweatt v. Painter (1950), Brown v. Board (1954), Davis v. County School Board Prince Edward County (1957), the legal decisions handed down in Steele v. Louisville and Nashville Railroad... ?Chapel Hill, 1????. N.F. Davis, ?Trade Unions’ Practices and the Negro Worker: The Establishment and Implementation of AFL-CIO Anti-Discrimination Policy,? (Ph.D. diss., Indiana University, 1960). Merle E. Reed, ?FEPC and the Federal Agencies...

  9. Spleen tyrosine kinase mediates high glucose-induced transforming growth factor-{beta}1 up-regulation in proximal tubular epithelial cells

    SciTech Connect (OSTI)

    Yang, Won Seok; Chang, Jai Won [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); Han, Nam Jeong [Department of Cell Biology, Asan Institute for Life Sciences, Seoul (Korea, Republic of)] [Department of Cell Biology, Asan Institute for Life Sciences, Seoul (Korea, Republic of); Lee, Sang Koo [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); Park, Su-Kil, E-mail: skpark@amc.seoul.kr [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)] [Division of Nephrology, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of)

    2012-09-10

    The role of spleen tyrosine kinase (Syk) in high glucose-induced intracellular signal transduction has yet to be elucidated. We investigated whether Syk is implicated in high glucose-induced transforming growth factor-{beta}1 (TGF-{beta}1) up-regulation in cultured human proximal tubular epithelial cells (HK-2 cell). High glucose increased TGF-{beta}1 gene expression through Syk, extracellular signal-regulated kinase (ERK), AP-1 and NF-{kappa}B. High glucose-induced AP-1 DNA binding activity was decreased by Syk inhibitors and U0126 (an ERK inhibitor). Syk inhibitors suppressed high glucose-induced ERK activation, whereas U0126 had no effect on Syk activation. High glucose-induced NF-{kappa}B DNA binding activity was also decreased by Syk inhibitors. High glucose increased nuclear translocation of p65 without serine phosphorylation of I{kappa}B{alpha} and without degradation of I{kappa}B{alpha}, but with an increase in tyrosine phosphorylation of I{kappa}B{alpha} that may account for the activation of NF-{kappa}B. Both Syk inhibitors and Syk-siRNA attenuated high glucose-induced I{kappa}B{alpha} tyrosine phosphorylation and p65 nuclear translocation. Depletion of p21-activated kinase 2 (Pak2) by transfection of Pak2-siRNA abolished high glucose-induced Syk activation. In summary, high glucose-induced TGF-{beta}1 gene transcription occurred through Pak2, Syk and subsequent ERK/AP-1 and NF-{kappa}B pathways. This suggests that Syk might be implicated in the diabetic kidney disease.

  10. Static-light mesons on a dynamical anisotropic lattice

    E-Print Network [OSTI]

    Justin Foley; Alan O Cais; Mike Peardon; Sinead M Ryan

    2006-10-09

    We present results for the spectrum of static-light mesons from Nf=2 lattice QCD. These results were obtained using all-to-all light quark propagators on an anisotropic lattice, yielding an improved signal resolution when compared to more conventional lattice techniques. In particular, we consider the inversion of orbitally-excited multiplets with respect to the `standard ordering', which has been predicted by some quark models.

  11. Slow crossover in YbXCu4 ,,XAg, Cd, In, Mg, Tl, Zn... intermediate-valence compounds J. M. Lawrence

    E-Print Network [OSTI]

    Lawrence, Jon

    of specific heat (T) C(T)/T, and 4f occupation number nf(T) for the intermediate-valence compounds YbXCu4 (X-Fermi-liquid behavior is neither expected nor observed and the scaling behavior that is ob- served does not reflect of as dispersive or bandlike behavior of the 4f electrons or alternatively as correlations between the 4f electrons

  12. Comparative physical limnology of farm ponds in Southcentral Texas 

    E-Print Network [OSTI]

    Meyers, Dewey Gregory

    1973-01-01

    COMPARATIVE PHYSICAL LIMNOLOGY OF FARM PONDS IN SOUTHCENTRAL TEXAS A Thesis by DEWEY GREGORY MEYERS Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE May... 1973 Ma)or Subject: Biology COMPARATIVE PHYSICAL LIMNOLOGY OF FARM PONDS IN SOUTHCENTRAL TEXAS A Thesis by DEWEY GREGORY MEYERS Approved as to style and content by: (Chairma of Committee) / i/y'/ /', . nf (Head 'of Depart'ment) (Member...

  13. High-Isolation Low-Power Active Quasi-Circulator for 44GHz Transceiver with Power and Noise Optimization in 0.18 Micrometer BICMOS 

    E-Print Network [OSTI]

    Meng, Fangyu

    2014-12-03

    with 3dB bandwidth of 4.53GHz, achieves maximum 2.897dBm input and 2.32dBm output power. Noise Figure (NF) is 10.62dB for reception path, only 0.03dB higher than NFmin. Linearity is reasonable for both in-phase divider and out-of-phase active combiner...

  14. Four-nucleon force in chiral effective field theory

    SciTech Connect (OSTI)

    Evgeny Epelbaum

    2005-10-25

    We derive the leading contribution to the four--nucleon force within the framework of chiral effective field theory. It is governed by the exchange of pions and the lowest--order nucleon--nucleon contact interaction and includes effects due to the nonlinear pion--nucleon couplings and the pion self interactions constrained by the chiral symmetry of QCD. The resulting 4NF does not contain any unknown parameters and can be tested in future few--and many--nucleon studies.

  15. Polyphenolics from Mango (Mangifera indica L.) and Pomegranate (Punica granatum L.) Suppress Inflammation in in vivo and in vitro Models for Colitis 

    E-Print Network [OSTI]

    Kim, Hye Mee

    2013-12-11

    and overproduction of pro?inflammatory cytokines, such as TNF-? and IL-6 (19, 20). Bifidobacterium and Lactobacillus are considered to be probiotics that induce health benefits, and exert anti-inflammatory properties through inhibition of NF??B activation and IL?8... of surviving passage through the gastrointestinal tract, and they produce SCFAs from bacterial fermentation in the colon. Unlike CD, UC may respond to probiotics, and the most common probiotics used in UC are Bifidobacteria, Lactobacillis, the E. coli strain...

  16. On irrationality and transcendency of infinite sums of rational numbers

    E-Print Network [OSTI]

    Tijdeman, Robert

    On irrationality and transcendency of infinite sums of rational numbers R. Tijdeman Abstract the irrationality and transcendency of sums n=1 nf(n) where Q, f(x) Q(x) such that f(n) is defined for all(n) where P(x), Q(x) Z[x]. We give conditions under which such a sum is irrational and under which

  17. Newsfront 23-29 July 2007, Issue 26

    E-Print Network [OSTI]

    Ghimire, Yubaraj

    Druk offered the cheapest service besides fulfilling other requirements. Toothless TRC soon ä nf correspondent The much awaited bill that will enable the government to form the Truth and Reconciliation Commission (TRC) as provided in the Comprehensive... for acquiring more and more electric power generation has never been greater. However, receiving a There is another revolution in the Nepali society and that is sexual revolution. In our patriarchal society, where women are still struggling to shatter the glass...

  18. The nature and formation of coke in the reaction of methanol to hydrocarbons over chabazite 

    E-Print Network [OSTI]

    McLaughlin, Kenneth Woot

    1983-01-01

    45 Figure 9. Relationship between enhanced coke formation with increasing yields of paraffins and diminishing yields of olefins 46 Figure 10. Gas chromatogram of the concentrated carbon tetrachloride extract of spent catalyst pellets . . . 49...THE NATURE ABD FORMATION OF COKE IB THE REACTIOB OF METHANOL TO HIDROCARBOBS OVER CHABAZITE A Thesis KENNETH WOOT MCLAUGHLLN Submitted to the Graduate College of Texas AAM Univers ty partial. fulfillment nf the req~nt fo~he degree of MASTER...

  19. 15 February 2012 Ay 21 E.S. Phinney Galaxies and Cosmology

    E-Print Network [OSTI]

    Phinney, E. Sterl

    . a) Integrate the Planck formula for the number density n and energy density u of photons of energy h in frequency interval d N = 2 42 c3 1 exp(h/kT) - 1 (1) b) Repeat for the number density nf and energy density the Saha equation (C&O 29.101) for a universe made of pure hydrogen to find the temperature when half

  20. Cottaneva Creek Ha rd yC re ek

    E-Print Network [OSTI]

    Blucher Cr Bear Cr Wolf Cr Ward Cr Fay Cr Billings Cr Mill Cr Annapolis Grape Cr Lorenzo R Scott Cr Big Cr of Big R Britain Cr Little Cr Bald Hill Cr Fly Cr Santa Rosa Cr Campbell Cr Rincon Cr Upper Indian Cr Big R Little N. Fork East of Eureka Hill Lower NF Big R Russell Bk Upper Navarro R Upper SF Ten Mile R

  1. Nepali Aawaz Volume 1, Issue 1, 5-12 October 2005

    E-Print Network [OSTI]

    Shrestha, Kashish Das

    |hftflGqs ;/sf/sf nflu lsdfy{ klg ;'xfpg] s'/f xf]Og . ;/sf/n] nfb]sf] o; k|ltjGwsf lj?4 g]kfnL rnlrqsdL{x?n] ;fgf] :j/df / gful/s ;dfhn] 7"n} :j/df lj/f]w u/]sf 5g\\ . cf]:sf/df g]kfnL rnlrq cg'kl:yt ;g\\ !((( df Sof/fefgnfO{ a]e]nL{ lxN;l:yt ;|fOg cl86f... lj/fdnfO{ ;/sf/n] p;sf] sdhf]/Lsf ?kdf lnP/ To;nfO{ ;s/fTds ?kdf glnbf o;kflnsf] bz} klg g/;+xf/ af6 d'Qm xf]nf eGg ;lsg] kl/l:ylt l;h{gf x'g g;s]sf] xf] . ufpF3/df zfxL g]kfnL ;]gf l;len 8«];df k;]/ dfcf]jfbLsf sfo{stf{x?nfO{ kqmfp ug{ yfn...

  2. Thymosin beta-4 promotes mesenchymal stem cell proliferation via an interleukin-8-dependent mechanism

    SciTech Connect (OSTI)

    Jeon, Byung-Joon; Yang, Yoolhee; Kyung Shim, Su; Yang, Heung-Mo; Cho, Daeho; Ik Bang, Sa

    2013-10-15

    Mesenchymal stem cells (MSCs) hold great promise for the field of tissue regeneration. Because only a limited number of MSCs can be obtained from each donor site, it is important to establish standard methods for MSC expansion using growth and trophic factors. Thymosin ?4 (T?4) is a novel trophic factor that has antimicrobial effects and the potential to promote tissue repair. T?4 is a ubiquitous, naturally-occurring peptide in the wound bed. Therefore, the relationship between T?4 and MSCs, especially adjacent adipose tissue-derived stem cells (ASCs), merits consideration. Exogenous T?4 treatment enhanced the proliferation of human ASCs, resulting in prominent nuclear localization of PCNA immunoreactivity. In addition, exogenous T?4 also increased IL-8 secretion and blocking of IL-8 with neutralizing antibodies decreased T?4-induced ASC proliferation, suggesting that IL-8 is a critical mediator of T?4-enhanced proliferation. Moreover, T?4 activated phosphorylation of ERK1/2 and increased the nuclear translocation of NF-?B. These observation provide that T?4 promotes the expansion of human ASCs via an IL-8-dependent mechanism that involves the ERK and NF-?B pathways. Therefore, T?4 could be used as a tool for MSC expansion in cell therapeutics. - Highlights: • This is fundamental information required to correlate T?4 with MSC expansion. • MSC expansion by T?4 is involved in enhancement of IL-8 and ERK/NF-?B pathway. • T?4 could be used as a tool for MSC expansion in cell therapeutics.

  3. Correlation ECE diagnostic in Alcator C-Mod

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Sung, C.; White, A. E.; Howard, N. T.; Mikkelsen, D.; Irby, J.; Leccacorvi, R.; Vieira, R.; Oi, C.; Rice, J.; Reinke, M.; et al

    2015-03-12

    Correlation ECE (CECE) is a diagnostic technique that allows measurement of small amplitude electron temperature, Te, fluctuations through standard cross-correlation analysis methods. In Alcator C-Mod, a new CECE diagnostic has been installed[Sung RSI 2012], and interesting phenomena have been observed in various plasma conditions. We find that local Te fluctuations near the edge (? ~ 0:8) decrease across the linearto- saturated ohmic confinement transition, with fluctuations decreasing with increasing plasma density[Sung NF 2013], which occurs simultaneously with rotation reversals[Rice NF 2011]. Te fluctuations are also reduced across core rotation reversals with an increase of plasma density in RF heated L-modemore »plasmas, which implies that the same physics related to the reduction of Te fluctuations may be applied to both ohmic and RF heated L-mode plasmas. In I-mode plasmas, we observe the reduction of core Te fluctuations, which indicates changes of turbulence occur not only in the pedestal region but also in the core across the L/I transition[White NF 2014]. The present CECE diagnostic system in C-Mod and these experimental results are described in this paper.« less

  4. Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice

    SciTech Connect (OSTI)

    Li, Weifeng Huang, Huimin; Niu, Xiaofeng Fan, Ting; Mu, Qingli; Li, Huani

    2013-10-01

    Excessive alcohol consumption can lead to gastric ulcer and the present work was aimed to examine the protective effect of tetrahydrocoptisine (THC) in the model of ethanol-induced gastric ulcer in mice. Fasted mice treated with ethanol 75% (0.5 ml/100 g) were pre-treated with THC (10 or 20 mg/kg, ip), cimetidine (100 mg/kg, ip) or saline in different experimental sets for a period of 3 days, and animals were euthanized 4 h after ethanol ingestion. Gross and microscopic lesions, immunological and biochemical parameters were taken into consideration. The results showed that ethanol induced gastric damage, improving nitric oxide (NO) level, increased pro-inflammatory cytokine (TNF-? and IL-6) levels and myeloperoxidase (MPO) activity, as well as the expression of nuclear factor-?B (NF-?B) in the ethanol group. Pretreatment of THC at doses of 10 and 20 mg/kg bodyweight significantly attenuated the gastric lesions as compared to the ethanol group. These results suggest that the gastroprotective activity of THC is attributed to reducing NO production and adjusting the pro-inflammatory cytokine, inhibited neutrophil accumulation and NF-?B expression. - Highlights: • THC decreased ethanol-induced pro-inflammatory cytokine release. • THC inhibited the production of NO in serum and gastric tissue. • THC reduced NF-?B expression and MPO accumulation in ethanol-induced gastric tissue.

  5. In vivo identification of promoter elements and transcription factors mediating activation of hepatic HMG-CoA reductase by T{sub 3}

    SciTech Connect (OSTI)

    Boone, Lindsey R.; Niesen, Melissa I. [Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, FL (United States)] [Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, FL (United States); Jaroszeski, Mark [Department of Chemical and Biomedical Engineering, College of Engineering, University of South Florida, Tampa, FL (United States)] [Department of Chemical and Biomedical Engineering, College of Engineering, University of South Florida, Tampa, FL (United States); Ness, Gene C., E-mail: gness@hsc.usf.edu [Department of Molecular Medicine, College of Medicine, University of South Florida, Tampa, FL (United States)

    2009-07-31

    The promoter elements and transcription factors necessary for triiodothyronine (T{sub 3}) induction of hepatic HMG-CoA reductase (HMGR) were investigated by transfecting rat livers with wild type and mutant HMGR promoter-luciferase constructs using in vivo electroporation. Mutations in the sterol response element (SRE), nuclear factor-y (NF-Y) site, and the newly identified upstream transcription factor-2 (USF-2) site essentially abolished the T{sub 3} response. Chromatin immunoprecipitation (ChIP) analysis demonstrated that T{sub 3} treatment caused a 4-fold increase in in vivo binding of USF-2 to the HMGR promoter. Co-transfection of the wild type HMGR promoter with siRNAs to USF-2, SREBP-2, or NF-Y nearly abolished the T{sub 3} induction, as measured by promoter activity. These data provide in vivo evidence for functional roles for USF-2, SREBP-2, and NF-Y in mediating the T{sub 3}-induction of hepatic HMGR transcription.

  6. Cancer Associated Fibroblasts express pro-inflammatory factors in human breast and ovarian tumors

    SciTech Connect (OSTI)

    Erez, Neta; Glanz, Sarah; Raz, Yael; Department of Obstetrics and Gynecology, LIS Maternity Hospital, Tel Aviv Sourasky Medical Center, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv ; Avivi, Camilla; Barshack, Iris; Department of Pathology, Sheba Medical Center, Tel Hashomer, affiliated with Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv

    2013-08-02

    Highlights: •CAFs in human breast and ovarian tumors express pro-inflammatory factors. •Expression of pro-inflammatory factors correlates with tumor invasiveness. •Expression of pro-inflammatory factors is associated with NF-?b activation in CAFs. -- Abstract: Inflammation has been established in recent years as a hallmark of cancer. Cancer Associated Fibroblasts (CAFs) support tumorigenesis by stimulating angiogenesis, cancer cell proliferation and invasion. We previously demonstrated that CAFs also mediate tumor-enhancing inflammation in a mouse model of skin carcinoma. Breast and ovarian carcinomas are amongst the leading causes of cancer-related mortality in women and cancer-related inflammation is linked with both these tumor types. However, the role of CAFs in mediating inflammation in these malignancies remains obscure. Here we show that CAFs in human breast and ovarian tumors express high levels of the pro-inflammatory factors IL-6, COX-2 and CXCL1, previously identified to be part of a CAF pro-inflammatory gene signature. Moreover, we show that both pro-inflammatory signaling by CAFs and leukocyte infiltration of tumors are enhanced in invasive ductal carcinoma as compared with ductal carcinoma in situ. The pro-inflammatory genes expressed by CAFs are known NF-?B targets and we show that NF-?B is up-regulated in breast and ovarian CAFs. Our data imply that CAFs mediate tumor-promoting inflammation in human breast and ovarian tumors and thus may be an attractive target for stromal-directed therapeutics.

  7. Reaching the chiral limit in many flavor systems

    E-Print Network [OSTI]

    Anna Hasenfratz; Anqi Cheng; Gregory Petropoulos; David Schaich

    2013-03-28

    We present a brief overview of our recent lattice studies of SU(3) gauge theory with N_f = 8 and 12 fundamental fermions, including some new and yet-unpublished results. To explore relatively unfamiliar systems beyond lattice QCD, we carry out a wide variety of investigations with the goal of synthesizing the results to better understand the non-perturbative dynamics of these systems. All our findings are consistent with conformal infrared dynamics in the 12-flavor system, but with 8 flavors we observe puzzling behavior that requires further investigation. Our new Monte Carlo renormalization group technique exploits the Wilson flow to obtain more direct predictions of a 12-flavor IR fixed point. Studies of N_f = 12 bulk and finite-temperature transitions also indicate IR conformality, while our current results for the 8-flavor phase diagram do not yet provide clear signs of spontaneous chiral symmetry breaking. From the Dirac eigenvalue spectrum we extract the mass anomalous dimension gamma_m, and predict gamma*_m = 0.32(3) at the 12-flavor fixed point. The N_f = 8 system again shows interesting behavior, with a large anomalous dimension across a wide range of energy scales. We use the eigenvalue density to predict the chiral condensate, and compare this approach with direct and partially-quenched measurements.

  8. Functional Toll-like receptor 4 expressed in lactotrophs mediates LPS-induced proliferation in experimental pituitary hyperplasia

    SciTech Connect (OSTI)

    Sabatino, María Eugenia; Sosa, Liliana del Valle; Petiti, Juan Pablo; Mukdsi, Jorge Humberto; Mascanfroni, Iván Darío; Pellizas, Claudia Gabriela; Gutiérrez, Silvina; Torres, Alicia Inés; De Paul, Ana Lucía

    2013-11-15

    Toll like receptor 4 (TLR4) has been characterized for its ability to recognize bacterial endotoxin lipopolysaccharide (LPS). Considering that infections or inflammatory processes might contribute to the progression of pituitary tumors, we analyzed the TLR4 functional role by evaluating the LPS effect on lactotroph proliferation in primary cultures from experimental pituitary tumors, and examined the involvement of PI3K-Akt and NF-?B activation in this effect. In addition, the role of 17?-estradiol as a possible modulator of LPS-induced PRL cell proliferation was further investigated. In estrogen-induced hyperplasic pituitaries, LPS triggered lactotroph cell proliferation. However, endotoxin failed to increase the number of lactotrophs taking up BrdU in normal pituitaries. Moreover, incubation with anti-TLR4 antibody significantly reduced LPS-induced lactotroph proliferation, suggesting a functional role of this receptor. As a sign of TLR4 activation, an LPS challenge increased IL-6 release in normal and tumoral cells. By flow cytometry, TLR4 baseline expression was revealed at the plasma membrane of tumoral lactotrophs, without changes noted in the percentage of double PRL/TLR4 positive cells after LPS stimulus. Increases in TLR4 intracellular expression were detected as well as rises in CD14, p-Akt and NF-?B after an LPS challenge, as assessed by western blotting. The TLR4/PRL and PRL/NF-?B co-localization was also corroborated by immunofluorescence and the involvement of PI3K/Akt signaling in lactotroph proliferation and IL-6 release was revealed through the PI3K inhibitor Ly-294002. In addition, 17?-estradiol attenuated the LPS-evoked increase in tumoral lactotroph proliferation and IL-6 release. Collectively these results demonstrate the presence of functional TLR4 in lactotrophs from estrogen-induced hyperplasic pituitaries, which responded to the proliferative stimulation and IL-6 release induced by LPS through TLR4/CD14, with a contribution of the PI3K-Akt and NF-?B signaling pathways. - Highlights: • In hyperplastic pituitaries, LPS triggered the lactotroph cell proliferation and IL-6 release. • Functional Toll-like receptor 4 (TLR4) is expressed at the plasma membrane of tumoral lactotrophs. • Increases in TLR4 and CD14 intracellular expression levels were detected after an LPS challenge. • The proliferative stimulation and IL-6 release involved the PI3K-Akt pathway and NF-?B activation. • 17?-estradiol attenuated the LPS-evoked tumoral lactotroph proliferation and IL-6 secretion.

  9. Macrophage-stimulating protein attenuates gentamicin-induced inflammation and apoptosis in human renal proximal tubular epithelial cells

    SciTech Connect (OSTI)

    Lee, Ko Eun [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Eun Young [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Chang Seong; Choi, Joon Seok; Bae, Eun Hui; Ma, Seong Kwon [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Kyung Keun [Department of Pharmacology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Pharmacology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Lee, Jong Un [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)] [Department of Physiology, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of); Kim, Soo Wan, E-mail: skimw@chonnam.ac.kr [Department of Internal Medicine, Chonnam National University Medical School, Gwangju 501-757 (Korea, Republic of)

    2013-05-10

    Highlights: •MSP/RON system is activated in rat kidney damaged by gentamicin. •MSP inhibits GM-induced cellular apoptosis and inflammation in HK-2 cells. •MSP attenuates GM-induced activation of MAPKs and NF-?B pathways in HK-2 cells. -- Abstract: The present study aimed to investigate whether macrophage-stimulating protein (MSP) treatment attenuates renal apoptosis and inflammation in gentamicin (GM)-induced tubule injury and its underlying molecular mechanisms. To examine changes in MSP and its receptor, recepteur d’origine nantais (RON) in GM-induced nephropathy, rats were injected with GM for 7 days. Human renal proximal tubular epithelial (HK-2) cells were incubated with GM for 24 h in the presence of different concentrations of MSP and cell viability was measured by MTT assay. Apoptosis was determined by flow cytometry of cells stained with fluorescein isothiocyanate-conjugated annexin V protein and propidium iodide. Expression of Bcl-2, Bax, caspase-3, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-?B), I?B-?, and mitogen-activated protein kinases (MAPKs) was analyzed by semiquantitative immunoblotting. MSP and RON expression was significantly greater in GM-treated rats, than in untreated controls. GM-treatment reduced HK-2 cell viability, an effect that was counteracted by MSP. Flow cytometry and DAPI staining revealed GM-induced apoptosis was prevented by MSP. GM reduced expression of anti-apoptotic protein Bcl-2 and induced expression of Bax and cleaved caspase 3; these effects and GM-induced expression of COX-2 and iNOS were also attenuated by MSP. GM caused MSP-reversible induction of phospho-ERK, phospho-JNK, and phospho-p38. GM induced NF-?B activation and degradation of I?B-?; the increase in nuclear NF-?B was blocked by inhibitors of ERK, JNK, p-38, or MSP pretreatment. These findings suggest that MSP attenuates GM-induced inflammation and apoptosis by inhibition of the MAPKs/NF-?B signaling pathways.

  10. Role of endoplasmic reticulum stress in acrolein-induced endothelial activation

    SciTech Connect (OSTI)

    Haberzettl, Petra; Vladykovskaya, Elena; Srivastava, Sanjay [Institute of Molecular Cardiology, Department of Medicine, University of Louisville, Louisville, KY 40202 (United States); Bhatnagar, Aruni [Institute of Molecular Cardiology, Department of Medicine, University of Louisville, Louisville, KY 40202 (United States)], E-mail: aruni@louisville.edu

    2009-01-01

    Acrolein is a ubiquitous environmental pollutant and an endogenous product of lipid peroxidation. It is also generated during the metabolism of several drugs and amino acids. In this study, we examined the effects of acrolein on endothelial cells. Treatment of human umbilical vein endothelial cells (HUVECs) with 2 to 10 {mu}M acrolein led to an increase in the phosphorylation of eIF-2{alpha} within 10 to 30 min of exposure. This was followed by alternate splicing of XBP-1 mRNA and an increase in the expression of the endoplasmic reticulum (ER) chaperone genes Grp78 and Herp. Within 2-4 h of treatment, acrolein also increased the abundance and the nuclear transport of the transcription factors ATF3, AFT4, and CHOP. Acrolein-induced increase in ATF3 was prevented by treating the cells with the chemical chaperone - phenylbutyric acid (PBA). Treatment with acrolein increased phosphorylation of ERK1/2, p38, and JNK. The increase in JNK phosphorylation was prevented by PBA. Acrolein treatment led to activation and nuclear translocation of the transcription factor NF-{kappa}B and an increase in TNF-{alpha}, IL-6 and IL-8, but not MCP-1, mRNA. Increased expression of cytokine genes and NF-{kappa}B activation were not observed in cells treated with PBA. These findings suggest that exposure to acrolein induces ER stress and triggers the unfolded protein response and that NF-{kappa}B activation and stimulation of cytokine production by acrolein could be attributed, in part, to ER stress. Chemical chaperones of protein-folding may be useful in treating toxicological and pathological states associated with excessive acrolein exposure or production.

  11. In vivo treatment with diphenyl ditelluride induces neurodegeneration in striatum of young rats: Implications of MAPK and Akt pathways

    SciTech Connect (OSTI)

    Heimfarth, Luana; Loureiro, Samanta Oliveira; Dutra, Márcio Ferreira; Andrade, Cláudia; Pettenuzzo, Letícia; Guma, Fátima T. Costa Rodrigues; Gonçalves, Carlos Alberto Saraiva [Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS (Brazil)] [Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS (Brazil); Batista Teixeira da Rocha, João [Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, RS Brazil (Brazil); Pessoa-Pureur, Regina, E-mail: rpureur@ufrgs.br [Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS (Brazil)] [Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS (Brazil)

    2012-10-15

    In the present report 15 day-old Wistar rats were injected with 0.3 ?mol of diphenyl ditelluride (PhTe){sub 2}/kg body weight and parameters of neurodegeneration were analyzed in slices from striatum 6 days afterwards. We found hyperphosphorylation of intermediate filament (IF) proteins from astrocyte (glial fibrillary acidic protein—GFAP and vimentin) and from neuron (low-, medium- and high molecular weight neurofilament subunits: NF-L, NF-M and NF-H, respectively) and increased MAPK (Erk, JNK and p38MAPK) as well as PKA activities. The treatment induced reactive astrogliosis in the striatum, evidenced by increased GFAP and vimentin immunocontent as well as their mRNA overexpression. Also, (PhTe){sub 2} significantly increased the propidium iodide (PI) positive cells in NeuN positive population without altering PI incorporation into GFAP positive cells, indicating that in vivo exposure to (PhTe){sub 2} provoked neuronal damage. Immunohistochemistry showed a dramatic increase of GFAP staining characteristic of reactive astrogliosis. Moreover, increased caspase 3 in (PhTe){sub 2} treated striatal slices suggested apoptotic cell death. (PhTe){sub 2} exposure decreased Akt immunoreactivity, however phospho-GSK-3-? (Ser9) was unaltered, suggesting that this kinase is not directly implicated in the neurotoxicity of this compound. Therefore, the present results shed light into the mechanisms of (PhTe){sub 2}-induced neurodegeneration in rat striatum, evidencing a critical role for the MAPK and Akt signaling pathways and disruption of cytoskeletal homeostasis, which could be related with apoptotic neuronal death and astrogliosis. -- Highlights: ? Diphenyl ditelluride causes apoptotic neuronal death in the striatum of young rats. ? Diphenyl ditelluride causes reactive astrogliosis in the striatum of rats. ? Diphenyl ditelluride disrupts the homeostasis of the cytoskeleton of the striatum. ? The actions of diphenyl ditelluride are mediated by MAPK and Akt signaling pathways.

  12. The role of annexin A1 in expression of matrix metalloproteinase-9 and invasion of breast cancer cells

    SciTech Connect (OSTI)

    Kang, Hyereen [Department of Medicine, Graduate School, University of Ulsan, Pungnap-2 dong, Songpa-gu, Seoul (Korea, Republic of)] [Department of Medicine, Graduate School, University of Ulsan, Pungnap-2 dong, Songpa-gu, Seoul (Korea, Republic of); Ko, Jesang [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)] [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of); Jang, Sung-Wuk, E-mail: swjang@amc.seoul.kr [Department of Medicine, Graduate School, University of Ulsan, Pungnap-2 dong, Songpa-gu, Seoul (Korea, Republic of)] [Department of Medicine, Graduate School, University of Ulsan, Pungnap-2 dong, Songpa-gu, Seoul (Korea, Republic of)

    2012-06-22

    Highlights: Black-Right-Pointing-Pointer We evaluated the effect of ANXA1 on promoting migration and invasion in MDA-MB-231 cells. Black-Right-Pointing-Pointer ANXA1 siRNA inhibits invasion and migration. Black-Right-Pointing-Pointer ANXA1 regulates MMP-9 expression and activity. Black-Right-Pointing-Pointer ANX-1 siRNA inhibits the activation of NF-{kappa}B in MDA-MB-231 cells. -- Abstract: Matrix metalloproteinase-9 (MMP-9) plays an important role in the invasion and metastasis of cancer cells. However, the regulatory mechanism of MMP-9 expression and its biological effects on breast cancer development remain obscure. In the current study, we examined the potential role of annexin A1 (ANXA1) in regulating migration and invasion in breast cancer cell lines. Both ANXA1 mRNA and protein are expressed in the highly invasive, hormone-insensitive human breast cancer cell lines MDA-MB-231 and SKBr3, but not in the hormone-responsive cell lines MCF-7 and T47D. Downregulation of ANXA1 expression with specific small interfering RNAs (ANXA1 siRNA) in MDA-MB-231 cells resulted in decreased cancer cell migration and invasion. Ablation of ANXA1 expression decreases the expression of MMP-9 at both the mRNA and protein levels and also reduces the proteolytic activity of MMP-9 in MDA-MB-231 cells. Moreover, silencing ANXA1 also decreases the transcriptional activity of MMP-9 by the suppression of nuclear factor kappa-B (NF-{kappa}B) activity. Collectively, these results indicate that ANXA1 functions as a positive regulator of MMP-9 expression and invasion of breast cancer cells through specific activation of the NF-{kappa}B signaling pathway.

  13. Bulletin of Tibetology: Volume 6 Number 3 : Notes and Topics

    E-Print Network [OSTI]

    Sinha, Nirmal Chandra

    . The Vajrachal ya (Rdo-rje slop-dpon of Padma-yang-t~e: the royal chaplain and presiding priest) offered to the Chogv.11, in iconic symbols, the Seven Jewels: Chakra (Di~Cll~), Chint.1mani (Wish fulfilling Gem), Mahishi (Cons Jrt), Mantri (Minister), Hasti... :ij q: I q'i:l{?t{T Cfi~l{~,~ !:nfUJ.,: RR oilS(r '9~Off;liHfl1f~ II There were several eminent ~cholars I ike Professor Suniti Kumar Chatter­ jee (India) and Professor P.H. Pott (Holland) who "witnessed the Coro...

  14. Approaching Conformality

    E-Print Network [OSTI]

    Maria Paola Lombardo; Kohtaroh Miura; Tiago Nunes da Silva; Elisabetta Pallante

    2014-11-06

    We investigate the preconformal region of the phase diagram of SU(3) theories with fundamental flavors. We have simulated SU(3) theories with six and eight fundamental flavors at volumes 32^3 x 64. We use the generated configurations to measure the string tension \\sigma and the w0 scale setting quantity extracted from the gradient flow. We show preliminary results on the ratios Tc/\\sqrt{\\sigma} and Tc w0. We compare them to the behavior obtained at smaller Nf and discuss the implications of our results.

  15. Baryon resonances coupled to Pion-Nucleon states in lattice QCD

    E-Print Network [OSTI]

    Valentina Verduci; Christian B. Lang

    2014-12-01

    In recent years the study of two particle systems on the lattice has led to excellent results in the meson sector of the QCD spectrum, however baryon resonances mostly remain unexplored. We present a study of pion-nucleon systems as decay product of baryon resonances in different channels, with special focus on the nucleon spectrum. We evaluate the correlation functions of single and multi particle interpolators. All the Wick contributions are explicitly computed and the consequences of reduced symmetries in moving frames are taken into account. We discuss the theoretical setup together with results for $n_f=2$ mass degenerate light quarks.

  16. Electromagnetic corrections to pseudoscalar decay constants

    E-Print Network [OSTI]

    Benjamin Glaessle; Gunnar S. Bali

    2011-11-16

    The effects of electromagnetic interactions on pseudoscalar decay constants are investigated. Using a compact QED and QCD action we are able to resolve differences of about 0.1 MeV. We obtain the preliminary results f_pi^0-f_pi^+/- =0.09(3) MeV and f_D^0-f_D^+/- =0.79(11) MeV for light and charmed pseudoscalar decay constants on a N_f=2 nonperturbatively improved Sheikholeslami-Wohlert ensemble.

  17. A comparative study of the mineral content of vitamin containing foods 

    E-Print Network [OSTI]

    Houston, James Maurice

    1936-01-01

    substituted for the spcaified ssmgie, 4, ". 'etsrniantlon nf ?hosphorue by:. epnrntion nn 'ngnesiukc . ', mmniun . hosphste nn4 . sti ?stion of the . hosphnte ns & hosphoi olybdnte Lhls mthod differs fro. " tkint sudkkcnted by yoe in tso ruspucts, 5 i... out, using 85 oc of - in place of ths sui~ostcd ner. i le for the aothnd outline4 hy toe. 11 10 ~ "otrruin~tion of '* nncsium ny . spar. tian es a;"cesium . ~as?, iete and sti! stion of thn, haspi ate as . hosphomrlybdars xcopt far usi, r ' co...

  18. 5d $E_n$ Seiberg-Witten curve via toric-like diagram

    E-Print Network [OSTI]

    Sung-Soo Kim; Futoshi Yagi

    2015-06-16

    We consider 5d Sp(1) gauge theory with $E_{N_f+1}$ global symmetries based on toric(-like) diagram constructed from (p,q)-web with 7-branes. We propose a systematic procedure to compute the Seiberg-Witten curve for generic toric-like diagram. For $N_f=6,7$ flavors, we explicitly compute the Seiberg-Witten curves for 5d Sp(1) gauge theory, and show that these Seiberg-Witten curves agree with already known $E_{7,8}$ results. We also discuss a generalization of the Seiberg-Witten curve to rank-N cases.

  19. Lineage selection and plasticity in the intestinal crypt

    E-Print Network [OSTI]

    Philpott, Anna; Winton, Douglas J.

    interest 1. Bjerknes M, Cheng H: Clonal analysis of mouse intestinal epithelial progenitors. Gastroenterology 1999, 116:7-14. 2. Yang Q, Bermingham NA, Finegold MJ, Zoghbi HY: Requirement of Math1 for secretory cell lineage commitment in the mouse intestine... and disease11. Shroyer NF, Helmrath MA, Wang VY, Antalffy B, Henning SJ, Zoghbi HY: Intestine-specific ablation of mouse atonal homolog 1 (Math1) reveals a role in cellular homeostasis. Gastroenterology 2007, 132:2478-2488. 12. van Es JH, van Gijn ME, Riccio O...

  20. An E7 Surprise

    E-Print Network [OSTI]

    Tudor Dimofte; Davide Gaiotto

    2012-09-06

    We explore some curious implications of Seiberg duality for an SU(2) four-dimensional gauge theory with eight chiral doublets. We argue that two copies of the theory can be deformed by an exactly marginal quartic superpotential so that they acquire an enhanced E7 flavor symmetry. We argue that a single copy of the theory can be used to define an E7-invariant superconformal boundary condition for a theory of 28 five-dimensional free hypermultiplets. Finally, we derive similar statements for three-dimensional gauge theories such as an SU(2) gauge theory with six chiral doublets or Nf=4 SQED.

  1. Bovine corpus luteum regression, estrus response and subsequent corpus luteum formation and function following treatment with Alfaprostol 

    E-Print Network [OSTI]

    Hansen, Thomas Ross

    1984-01-01

    and appeared to counteract the inhibitory i nf I uence of. PGF. Obviously, the r coul ation of luteal function revolves around the relative pro- portions and sensitivities of small and large luteal cells. Indeed, the proportion of small to large luteal... receptors) at this time (42). Cor us Luteum R ression The i denti ffcation oF PGF as the 1 uteol ytfc factor revolved around early work with the uterus. A local effect oF the uterus upon corpus 1 uteum maintenance in guf nea pigs was First observed...

  2. Production and characterisation of hydroxyapatite/multi-walled carbon nanotube composites

    E-Print Network [OSTI]

    White, Ashley Ann

    2010-01-12

    transform infrared spectroscopy HA Hydroxyapatite HIP Hot isostatic pressing MWNT Multi-walled carbon nanotube nfCNT Non-functionalised carbon nanotube SDT Simultaneous DSC/TGA SEM Scanning electron microscope/microscopy SPS Spark plasma sintering... diameter (Yu, M. F. et al. 2000). The elastic modulus of CNTs is estimated at greater than 1 TPa, based on the in-plane elastic modulus of graphite. This value has been confirmed experimentally on arc-grown CNTs by using atomic force microscopy (Falvo et...

  3. Horticultural Characters of Tomatoes. 

    E-Print Network [OSTI]

    MacArthur, J. W. (James William); Young, P. A. (Paul Allen)

    1947-01-01

    , TEXAS AGRICULTURAL EXPERIMENT STATION Figure 1. A: Tomato plant, G1078, is bushy due to the bu-allele and is densely compact in habit of growth in this pre-bloom stage when com- mercial tomatoes (as exemplified by T870) have long stems and petioles... of tomatoes that were tested at the Tomato Disease Laboratory (Figure 3,A). This small-leaf character vi7as associated with macrocalyx, 2-locule fruits and single-f ruit trusses. -nf roll. When Blair Forcing variety of greenliouse tomato 5r0nr11 in fields...

  4. Nuclear magnetic resonances in weak fields 

    E-Print Network [OSTI]

    Mitchell, Richard Warren

    1953-01-01

    nosent per unit votune~ is related to p ~ the relation A h rp u 5I ~ 71 y, ths constant appearing in (3), is the gyroauupxetie ratio of the nu?ious+ Conbination of {2) and (3) yields the equation d8/dt e 8'8 ~ He A Rquati?n (4) with the value... whish depends on the dif fersnoe between 8? and Ho. kssuadng that the rate nf ?hangs of 8 is proportdonal to (8 Ha)x it follows that d/dt (8, - Ho) s 1/Tl (Ha - 8, ), where Tl is a constant having the dimensions of time. Upon integration of (6), Tl...

  5. Humic acid in drinking well water induces inflammation through reactive oxygen species generation and activation of nuclear factor-?B/activator protein-1 signaling pathways: A possible role in atherosclerosis

    SciTech Connect (OSTI)

    Hseu, You-Cheng [Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan (China); Department of Molecular and Cellular Oncology, University of Texas, MD Anderson Cancer Center, TX 77030 (United States); Senthil Kumar, K.J. [Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan (China); Chen, Chih-Sheng; Cho, Hsin-Ju; Lin, Shu-Wei; Shen, Pei-Chun [Institute of Nutrition, China Medical University, Taichung 40402, Taiwan (China); Lin, Cheng-Wen [Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan (China); Lu, Fung-Jou [Institute of Medicine, Chun Shan Medical University, Taichung 40201, Taiwan (China); Yang, Hsin-Ling, E-mail: hlyang@mail.cmu.edu.tw [Institute of Nutrition, China Medical University, Taichung 40402, Taiwan (China); Department of Molecular and Cellular Oncology, University of Texas, MD Anderson Cancer Center, TX 77030 (United States)

    2014-01-15

    Humic acid (HA) has been implicated as one of the etiological factors in the peripheral vasculopathy of blackfoot disease (BFD) in Taiwan. However, the underlying pathophysiological mechanisms of BFD are not well defined. In this study, we used an in vitro and in vivo model, in which HA (25–200 ?g/mL) activated macrophages to produce pro-inflammatory molecules by activating their transcriptional factors. HA exposure induced NO and PGE{sub 2} production followed by induction of iNOS and COX-2 through NF-?B/AP-1 transactivation in macrophages. In addition, the production of TNF-? and IL-1? was significantly increased by HA. Moreover, HA-induced iNOS and COX-2 expression were down-regulated by the NF-?B and AP-1 inhibitors pyrrolidine dithiocarbamate (PDTC) and Tanshinone, respectively. Furthermore, generations of ROS and nitrotyrosine, as well as activation of the AKT and MAPKs signaling cascades were observed after HA exposure. Specifically, HA-induced NF-?B activation was mediated by ROS and AKT, and that HA-induced AP-1 activation was mediated by JNK and ERK. Notably, HA-mediated AKT, JNK, and ERK activation was ROS-independent. The inflammatory potential of HA was correlated with increased expression of HO-1 and Nrf2. Furthermore, an in vivo study confirms that mice exposed to HA, the serum levels of TNF-? and IL-1? was significantly increased in a dose-dependent manner. This report marks the first confirmation that environmental exposure of HA induces inflammation in macrophages, which may be one of the main causes of early atherogenesis in blackfoot disease. - Highlights: • Humic acid (HA) induce pro-inflammatory cytokines and mediators in macrophages. • HA-induced inflammation is mediated by ROS and NF-?B/AP-1 signaling pathways. • The inflammatory potential of HA correlated with activation of Nrf2/HO-1 genes. • HA exposure to mice increased pro-inflammatory cytokines production in vivo. • HA may be one of the main causes of early atherogenesis in blackfoot disease.

  6. Static-light meson-meson potentials

    E-Print Network [OSTI]

    Gunnar Bali; Martin Hetzenegger

    2010-11-02

    We investigate potentials between pairs of static-light mesons in Nf=2 Lattice QCD, in different spin channels. The question of attraction and repulsion is particularly interesting with respect to the X(3872) charmonium state and charged candidates such as the Z+(4430). We employ the nonperturbatively improved Sheikholeslami-Wohlert fermion and the Wilson gauge actions at a lattice spacing a approx. 0.084 fm and a pseudoscalar mass mPS approx. 760 MeV. We use stochastic all-to-all propagator techniques, improved by a hopping parameter expansion. The analysis is based on the variational method, utilizing various source and sink interpolators.

  7. The static-light meson spectrum from twisted mass lattice QCD

    E-Print Network [OSTI]

    ETM Collaboration; Karl Jansen; Chris Michael; Andrea Shindler; Marc Wagner

    2008-10-10

    We compute the static-light meson spectrum with N_f = 2 flavours of sea quarks using Wilson twisted mass lattice QCD. We consider five different values for the light quark mass corresponding to 300 MeV < m_PS < 600 MeV and we present results for angular momentum j = 1/2, j = 3/2 and j = 5/2 and for parity P = + and P = -. We extrapolate our results to physical quark masses and make predictions regarding the spectrum of B and B_s mesons.

  8. hp-Adaptive Multi-Physics Finite-Element Simulation of Wireline Borehole Sonic Waveforms Pawel J. Matuszyk and Carlos Torres-Verdin, University of Texas at Austin

    E-Print Network [OSTI]

    Torres-Verdín, Carlos

    ,q A - f 2 n q,nf ·u I = gex,q ex Cu,w E - s2 n u,w E + pns,w I = 0, where p(x,n) is a Fourier transform is posed in the frequency domain and solved for a particular angular frequency n. Accordingly, weak the form of the coupled problem reads as Find (u, p) (¯u, ¯p)+H1 0,D(E)×H1 0,D(A) : (1) p,q A - k2 n p

  9. {moheo, kikim, slee, btzhang}@bi.snu.ac.kr An Encoding Method of Sequential Patterns using Energy-based

    E-Print Network [OSTI]

    N k f f f k t t N t t t t k f E h X X W - - - - + = = = = - - h X 1 t-1 t W , , Nk . Nf . X1:T . 1 1 , 2 1: 1 1: 1 1 1 1 1 0 ( , ) (( ) ) fw k W w NT N N N k f f f k T N T t t t t k f E h X X W - + - - + - - - + = = = = = - - h X , ht k ht k 0 1 , sigmoid . : : : exp( ( , )) ( 1| 0, ) 1 exp( ( , )) W W W t t N tk k t t t N

  10. Thermodynamics using p4-improved staggered fermion action on QCDOC

    E-Print Network [OSTI]

    Chulwoo Jung

    2005-10-05

    We present an exploratory study of the thermodynamics of $N_f=3$ QCD with an improved staggered fermions using the QCDOC supercomputer. We use a p4 action with MILC-style smeared links (Fat 7). Some details of the implementation of the p4 action on QCDOC are discussed and performance benchmarks are given. We show preliminary results for the quark mass dependence of the pseudo-critical temperature $T_c$ from several lattice volumes . We also make a comparison between p4fat7 and the old p4 action.

  11. Exposure to As, Cd and Pb-mixture impairs myelin and axon development in rat brain, optic nerve and retina

    SciTech Connect (OSTI)

    Rai, Nagendra Kumar; Ashok, Anushruti; Rai, Asit; Tripathi, Sachin; Nagar, Geet Kumar; Mitra, Kalyan; Bandyopadhyay, Sanghamitra

    2013-12-01

    Arsenic (As), lead (Pb) and cadmium (Cd) are the major metal contaminants of ground water in India. We have reported the toxic effect of their mixture (metal mixture, MM), at human relevant doses, on developing rat astrocytes. Astrocyte damage has been shown to be associated with myelin disintegration in CNS. We, therefore, hypothesized that the MM would perturb myelinating white matter in cerebral cortex, optic nerve (O.N.) and retina. We observed modulation in the levels of myelin and axon proteins, such as myelin basic protein (MBP), proteolipid protein, 2?-, 3?-cyclic-nucleotide-3?-phosphodiesterase, myelin-associated glycoprotein and neurofilament (NF) in the brain of developing rats. Dose and time-dependent synergistic toxic effect was noted. The MBP- and NF-immunolabeling, as well as luxol-fast blue (LFB) staining demonstrated a reduction in the area of intact myelin-fiber, and an increase in vacuolated axons, especially in the corpus-callosum. Transmission electron microscopy (TEM) of O.N. revealed a reduction in myelin thickness and axon-density. The immunolabeling with MBP, NF, and LFB staining in O.N. supported the TEM data. The hematoxylin and eosin staining of retina displayed a decrease in the thickness of nerve-fiber, plexiform-layer, and retinal ganglion cell (RGC) count. Investigating the mechanism revealed a loss in glutamine synthetase activity in the cerebral cortex and O.N., and a fall in the brain derived neurotrophic factor in retina. An enhanced apoptosis in MBP, NF and Brn3b-containing cells justified the diminution in myelinating axons in CNS. Our findings for the first time indicate white matter damage by MM, which may have significance in neurodevelopmental-pediatrics, neurotoxicology and retinal-cell biology. - Highlights: • As, Cd and Pb-mixture, at human relevant dose, demyelinate developing rat CNS. • The attenuation in myelin and axon is synergistic. • The optic nerve and brain demonstrate reduced glutamine synthetase. • The retina exhibits diminished neurotrophin levels and cellular differentiation. • The toxic effect is apoptotic.

  12. The use of oxidation-reduction dyes in the determination of the shelf-life of meats 

    E-Print Network [OSTI]

    Bush, Janis Carolyn

    1970-01-01

    and placed in 50 ml Bacto- rept&?! . . This suspension !ras t!! e used beth fnr the nus&erical estr- n, &ti on aud for &. hv dyc. redo? i?n& test . . . A 9 ml p&sction nf bc th thc m a' emulsion and the soaper!srnn from th- p!Dvr was pipe t ted in& o s..., 1970) Janis C. Bush, B. S. (Biology), Abilene Christian College Directed by: Dr. Carl Vanderzant A technique w's developed to carry out dye reduction tests with a m'nimum ot interference of tissu- in the reduction process. Tne technique e...

  13. The two flavour Schwinger model: scaling of the scalar condensate

    E-Print Network [OSTI]

    Kei-ichi Nagai; Nils Christian; Karl Jansen; Beatrix Pollakowski

    2007-11-15

    We investigate the continuum limit scaling of the scalar condensate in the $N_f=2$ Schwinger model on the lattice. We employ maximally twisted mass Wilson fermions and overlap fermions. We compute the scalar condensate by taking the trace of the propagator (direct method) and by utilizing the integrated Ward-Takahashi identity. While the scalar condensate comes out consistent using these two methods for a given kind of lattice fermions, we find --quite surprisingly-- large discrepancies for the scalar condensate between twisted mass and overlap fermions. These discrepancies are only resolved when using the point split current for twisted mass fermions.

  14. An Anthropomorphized Version of McCune's Machine Proof that Robbins Algebras are Boolean Algebras

    E-Print Network [OSTI]

    McCune, William

    variables in the term, i.e., one obtains t(x; x; x; : : :). A := x + y c A := x + x B := N(x + y) C := Nx + y D := N(Nx+ y) c D := N(Nx + x) E := D +A c E := c D + c A F := E + y G := F +D H := NF +D I := H (C) x + (y + z) = (x + y) + z (A) N[N(Nx + y) +N(x + y)] = y (RX): PROOF N[N(x + y) +N(Nx + y)] = y

  15. Charge Storage in Organic Electrodes for Energy & Electrochemical Applications 

    E-Print Network [OSTI]

    Jeon, Ju Won

    2014-10-15

    ………………………………………………………………...………138 vii LIST OF FIGURES Page 1.1 Various cathode and anode materials with their potential and capacity..……...……3 1.2 Schematic illustration of the discharge and charge processes of a lithium rechargeable battery....13 Graphs of log i vs. log ? for (a) anodic and (b) cathodic scans of the 347 nm thick (PANI-NF2.5/ERGO3.5) LbL electrodes used to obtain b values. The calculation was performed using cyclic voltammograms from 1 to 5 mV/s….....101 4.14 Calculated b...

  16. Chow Wagons 

    E-Print Network [OSTI]

    Unknown

    2011-09-05

    . Horlacher, Ph. D., Genetics D. Scoates, A. E.. Agricultural Engineering J. H. Knox, M. S., Animal Husbandry A. K. Mackey. M. S., Animal Husbandry A. L. Darnell, M. A., Dairy Husbandry *Dean School of Veterinary Medicine. ?As of March 1. 1932. **In coo... difference of $12.17 in favor machine harvesting. !on- ton. ent :ive the 'er - ,C V1 ant a re- . . ars 'ull In nd - YZU In age tad. de. ale ith nf 11Vb ste- nic- BULLETIN NO. 452 AUGUST, 1932 THE MECHANICAL HARVESTING OF COTTON H...

  17. First results from $2+1$ dynamical quark flavors on an anisotropic lattice: light-hadron spectroscopy and setting the strange-quark mass

    SciTech Connect (OSTI)

    Lin, Huey-Wen; Cohen, Saul; Dudek, Jozef; Edwards, Robert; Joo, Balint; Richards, David; Bulava, John; Foley, Justin; Morningstar, Colin; Engelson, Eric; Wallace, Stephen; Juge, Jimmy; Mathur, Nilmani; Peardon, Michael; Ryan, Sinead

    2009-02-01

    We present the first light-hadron spectroscopy on a set of $N_f=2+1$ dynamical, anisotropic lattices. A convenient set of coordinates that parameterize the two-dimensional plane of light and strange-quark masses is introduced. These coordinates are used to extrapolate data obtained at the simulated values of the quark masses to the physical light and strange-quark point. A measurement of the Sommer scale on these ensembles is made and the performance of the hybrid Monte Carlo algorithm used for generating the ensembles is estimated.

  18. HIGH ABUNDANCES OF PRESOLAR SILICATES IN ANTARCTIC MICROMETEORITES; IMPLICATIONS FOR THEIR COMETARY ORIGINS. T. Yada1,2,3

    E-Print Network [OSTI]

    Syowa Station, in East Antarctica [8, 9]. Seven fine-grained AMMs, T98NF2, -H3, -H5, -G6, -G8, TT54B397 silicates in T98H5 (grains 1P1, 4P1 and 15P1), three Group 4 presolar silicates in T98G8 (grains 4P1, 8P1 been revised to be 50 ppm. For three presolar-silicate-bearing AMMs, TT54B397, T98H5, and ­G8

  19. Newsfront 14-20 May 2007, Issue 16

    E-Print Network [OSTI]

    Ghimire, Yubaraj

    Oil Corporation would speed up the process of clearing in part the 5.4 billion Rupees that it owes to the Indian Oil Corpora- tion (IOC). But the meeting also focused on the Terai problem and Goit group setting up a base in India. However, Goit’s India... of solidarity with the government engineers in the country who are demanding arrests ä nf correspondent The Eight Party Alliance (EPA) has failed to meet for more than a month now with at least three scheduled meetings postponed for some reason or the other...

  20. Pseudoscalar singlet physics with staggered fermions

    E-Print Network [OSTI]

    Eric B. Gregory; Alan C. Irving; Craig McNeile; Steven Miller; Zbigniew Sroczynski

    2005-09-30

    We report on progress in measuring disconnected correlators associated with pseudoscalar flavor-singlet mesons. This will eventually allow us to compute the masses of the eta and eta' mesons. Flavor-singlet physics also presents an interesting test of the staggered fermion formulation, as disconnected correlators are sensitive to whether the same action governs both sea quarks and valence quarks. It can also help test the validity of the ``fourth-root trick'' used in unquenched lattice calculations where the number of flavors $N_f<4$.

  1. Elastic/plastic deformation and fracture of annealed Ni??.??Si?.?B?.?C?.?? metallic glass 

    E-Print Network [OSTI]

    Harbert, Stuart John

    1987-01-01

    ELASTIC/PLASTIC DEFORMATION AND FRACTURE OF ANNEALED Nf gs s4 St4, sB4 sCp ps METALLIC GLASS A Thesis STUART 3OIIN IIARBERT Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirement for the degree of..., three distinct failure regions are discerned, including a slipped region, a veined fracture region, and a ductile, tearing region. At an annealing tempera. ture of 375s C and above, a fourth failure region was found, which was the development of a...

  2. Leptonic B- and D-meson decay constants with 2+1 flavors of asqtad fermions

    E-Print Network [OSTI]

    Andreas S. Kronfeld; Ethan T. Neil; James N. Simone; Ruth S. Van de Water; for the Fermilab Lattice Collaboration; for the MILC Collaboration

    2015-01-08

    We present the status of our updated D- and B-meson decay-constant analysis, based on the MILC $N_f = 2+1$ asqtad gauge ensembles. Heavy quarks are incorporated using the Wilson clover action with the Fermilab interpretation. This analysis includes ensembles at five lattice spacings from a $\\approx$ 0.045 to 0.15 fm, and light sea-quark masses down to 1/20th of the strange-quark mass. Projected error budgets for ratios of decay constants, in particular between bottom- and charm-meson decay constants, are presented.

  3. Yield enhancement of VLSI/WSI array systems 

    E-Print Network [OSTI]

    Koo, Peter Yunemo

    1989-01-01

    Xs X7 F1g. 12 Detection nf br1ciging faults between two output 11nes. Table V. Statistics of the proposed approach for 5-3-3 fault patter&i PLA input product output PCD, PD, PCD?PD, 3 F, adr4 lines lines lines 92. 13 0. 33 96 04 1 Or (%) 94... single wafer, tllils avoiding lugli manufacturing costs. In this research, vield enhancement of two typical VLSI/IVSI array systems, PLAs aud Pipeline 4rrays, is considered Yield enhancement of PLAs tluough reconfiguration is an important issue...

  4. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,6037 554,"Ames City of",6,2,"MidAmerican

  5. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,6037 554,"Ames City

  6. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,6037 554,"Ames CityOT",333,9,,14413,14413

  7. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,6037 554,"Ames

  8. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,6037

  9. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,60371,0,0,0,0,"Steam Power Generation (500-507,

  10. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,60371,0,0,0,0,"Steam Power Generation

  11. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,60371,0,0,0,0,"Steam Power

  12. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home PageMonthly","10/2015"4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,60371,0,0,0,0,"Steam

  13. Section 27

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust, High-ThroughputUpcomingmagnetoresistanceand Governmentm D m r e D m5 AtmosphericExpertNF

  14. Chairman John Deutch

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirley Ann JacksonDepartment|Marketing, LLCEfficiencyCOP 21:Department of Energy U.S.otnngress nf

  15. Effective Temperature in Steady-state Dynamics from Holography

    E-Print Network [OSTI]

    Kundu, Arnab

    2015-01-01

    We argue that, within the realm of gauge-gravity duality, for a large class of systems in a steady-state there exists an effective thermodynamic description. This description comes equipped with an effective temperature and a free energy, but no well-defined notion of entropy. Such systems are described by probe degrees of freedom propagating in a much larger background, e.g. $N_f$ number of ${\\cal N} =2$ hypermultiplets in ${\\cal N}=4$ $SU(N_c)$ super Yang-Mills theory, in the limit $N_f \\ll N_c$. The steady-state is induced by exciting an external electric field that couples to the hypermultiplets and drives a constant current. With various stringy examples, we demonstrate that an open string equivalence principle determines a unique effective temperature for all fluctuations in the probe-sector. We further discuss various properties of the corresponding open string metric that determines the effective geometry which the probe degrees of freedom are coupled to. We also comment on the non-Abelian generalizat...

  16. A multichannel magnetic probe system for analysing magnetic fluctuations in helical axis plasmas

    SciTech Connect (OSTI)

    Haskey, S. R.; Blackwell, B. D.; Seiwald, B.; Hole, M. J.; Pretty, D. G.; Howard, J.; Wach, J. [Plasma Research Laboratory, Research School of Physical Sciences and Engineering, The Australian National University, Canberra, ACT 0200 (Australia)] [Plasma Research Laboratory, Research School of Physical Sciences and Engineering, The Australian National University, Canberra, ACT 0200 (Australia)

    2013-09-15

    The need to understand the structure of magnetic fluctuations in H-1NF heliac [S. Hamberger et al., Fusion Technol. 17, 123 (1990)] plasmas has motivated the installation of a sixteen former, tri-axis helical magnetic probe Mirnov array (HMA). The new array complements two existing poloidal Mirnov arrays by providing polarisation information, higher frequency response, and improved toroidal resolution. The helical placement is ideal for helical axis plasmas because it positions the array as close as possible to the plasma in regions of varying degrees of favourable curvature in the magnetohydrodynamic sense, but almost constant magnetic angle. This makes phase variation with probe position near linear, greatly simplifying the analysis of the data. Several of the issues involved in the design, installation, data analysis, and calibration of this unique array are presented including probe coil design, frequency response measurements, mode number identification, orientation calculations, and mapping probe coil positions to magnetic coordinates. Details of specially designed digitally programmable pre-amplifiers, which allow gains and filters to be changed as part of the data acquisition initialisation sequence and stored with the probe signals, are also presented. The low shear heliac geometry [R. Jiménez-Gómez et al., Nucl. Fusion 51, 033001 (2011)], flexibility of the H-1NF heliac, and wealth of information provided by the HMA create a unique opportunity for detailed study of Alfvén eigenmodes, which could be a serious issue for future fusion reactors.

  17. Repulsive nature of optical potentials for high-energy heavy-ion scattering

    E-Print Network [OSTI]

    T. Furumoto; Y. Sakuragi; Y. Yamamoto

    2010-10-08

    The recent works by the present authors predicted that the real part of heavy-ion optical potentials changes its character from attraction to repulsion around the incident energy per nucleon E/A = 200 - 300 MeV on the basis of the complex G-matrix interaction and the double-folding model (DFM) and revealed that the three-body force plays an important role there. In the present paper, we have precisely analyzed the energy dependence of the calculated DFM potentials and its relation to the elastic-scattering angular distributions in detail in the case of the $^{12}$C + $^{12}$C system in the energy range of E/A = 100 - 400 MeV. The tensor force contributes substantially to the energy dependence of the real part of the DFM potentials and plays an important role to lower the attractive-to-repulsive transition energy. The nearside and farside (N/F) decomposition of the elastic-scattering amplitudes clarifies the close relation between the attractive-to-repulsive transition of the potentials and the characteristic evolution of the calculated angular distributions with the increase of the incident energy. Based on the present analysis, we propose experimental measurements of the predicted strong diffraction phenomena of the elastic-scattering angular distribution caused by the N/F interference around the attractive-to-repulsive transition energy together with the reduced diffractions below and above the transition energy.

  18. Orientifold Planar Equivalence: The Chiral Condensate

    E-Print Network [OSTI]

    Adi Armoni; Biagio Lucini; Agostino Patella; Claudio Pica

    2008-09-29

    The recently introduced orientifold planar equivalence is a promising tool for solving non-perturbative problems in QCD. One of the predictions of orientifold planar equivalence is that the chiral condensates of a theory with $N_f$ flavours of Dirac fermions in the symmetric (or antisymmetric) representation and $N_f$ flavours of Majorana fermions in the adjoint representation have the same large $N$ value for any value of the mass of the (degenerate) fermions. Assuming the invariance of the theory under charge conjugation, we prove this statement on the lattice for staggered quenched condensates in SU($N$) Yang-Mills in the large $N$ limit. Then, we compute numerically those quenched condensates for $N$ up to 8. After separating the even from the odd corrections in $1/N$, we are able to show that our data support the equivalence; however, unlike other quenched observables, subleading terms in $1/N$ are needed for describing the data for the symmetric and antisymmetric representation at $N$=3. Possible lessons for the unquenched case are discussed.

  19. Isotopic chains around oxygen from evolved chiral two- and three-nucleon interactions

    E-Print Network [OSTI]

    Andrea Cipollone; Carlo Barbieri; Petr Navrátil

    2013-06-21

    We extend the formalism of self-consistent Green's function theory to include three-body interactions and apply it to isotopic chains around oxygen for the first time. The third-order algebraic diagrammatic construction [ADC(3)] equations for two-body Hamiltonians can be exploited upon defining system-dependent one- and two-body interactions coming from the three-body force, and correspondingly dropping interaction reducible diagrams. This goes beyond the standard normal ordering truncations recently used in ab-inito studies. The Koltun sum rule for the total binding energy acquires a correction due to the added three-body interaction. This formalism is then applied to study chiral two-nucleon (2N) and three-nucleon forces (3NF) evolved to low momentum cutoffs. We find that these interactions reproduce the binding energies of nitrogen, oxygen and fluorine isotopes to great accuracy, providing clear indication of the predictive power of this approach. All three neutron driplines are correctly predicted when full 3NF are included. The formalism introduced also allows to calculate form factors for nucleon transfer on doubly magic systems.

  20. Etching process of silicon dioxide with nonequilibrium atmospheric pressure plasma

    SciTech Connect (OSTI)

    Yamakawa, Koji; Hori, Masaru; Goto, Toshio; Den, Shoji; Katagiri, Toshirou; Kano, Hiroyuki [Department of Quantum Engineering, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603 (Japan); Department of Electrical Engineering and Computer Science, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603 (Japan); Department of Quantum Engineering, Nagoya University, Furo-Cho, Chikusa-ku, Nagoya 464-8603 (Japan); Department of Engineering, Katagiri Engineering Co., Ltd., Minamikase Saiwai-ku, Kawasaki 661-8661 (Japan); NU-EcoEngineering Co., Ltd., Ooaza, Kurozasa Aza, Umazutsumi, Miyoshi-cho, Nishikamo-gun, Aichi 470-0201 (Japan)

    2005-07-01

    An ultrahigh etch rate (14 {mu}m/min) of SiO{sub 2} and a high selectivity of SiO{sub 2}/Si over 200 were achieved using a microwave-excited nonequilibrium atmospheric pressure plasma source employing He, NF{sub 3}, and H{sub 2}O gases, which have been developed for application to microelectromechanical systems and other bionanotechnology fields. In order to clarify the etching mechanism, two diagnostic methods have been performed: (1) imaging of plasma emission with an intensified charge-coupled device camera, and (2) absorption measurements using Fourier transform infrared spectroscopy. The etching characteristics are discussed in relation to the spatial distributions of the species involved. The etch rate depended considerably on the distance between the plasma and the substrate. Some radicals generated from the feed gases reached the substrate directly, while other radicals recombined into different species, which reached the substrate. An abundance of HF molecules were produced through a reaction between radicals generated by the atmospheric pressure discharge of NF{sub 3} and H{sub 2}O. From these measurements, it has been found that the HF molecules generated played a role in producing the high etch rate of SiO{sub 2} and high etch selectivity of SiO{sub 2}/Si.

  1. Id1 expression promotes peripheral CD4{sup +} T cell proliferation and survival upon TCR activation without co-stimulation

    SciTech Connect (OSTI)

    Liu, Chen; Jin, Rong [Department of Immunology, Peking University Health Science Center, Beijing (China)] [Department of Immunology, Peking University Health Science Center, Beijing (China); Wang, Hong-Cheng [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States)] [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States); Tang, Hui; Liu, Yuan-Feng; Qian, Xiao-Ping; Sun, Xiu-Yuan; Ge, Qing [Department of Immunology, Peking University Health Science Center, Beijing (China)] [Department of Immunology, Peking University Health Science Center, Beijing (China); Sun, Xiao-Hong, E-mail: sunx@omrf.org [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States)] [Oklahoma Medical Research Foundation, Oklahoma City, OK (United States); Zhang, Yu, E-mail: zhangyu007@bjmu.edu.cn [Department of Immunology, Peking University Health Science Center, Beijing (China)] [Department of Immunology, Peking University Health Science Center, Beijing (China)

    2013-06-21

    Highlights: •Id1 expression enables naïve T cell proliferation without anti-CD28 co-stimulation. •Id1 expression facilitates T cells survival when stimulated with anti-CD3. •Elevation of IL-2 production by Id1 contributes increased proliferation and survival. •Id1 potentiates NF-?B activation by anti-CD3 stimulation. -- Abstract: Although the role of E proteins in the thymocyte development is well documented, much less is known about their function in peripheral T cells. Here we demonstrated that CD4 promoter-driven transgenic expression of Id1, a naturally occurring dominant-negative inhibitor of E proteins, can substitute for the co-stimulatory signal delivered by CD28 to facilitate the proliferation and survival of naïve CD4{sup +} cells upon anti-CD3 stimulation. We next discovered that IL-2 production and NF-?B activity after anti-CD3 stimulation were significantly elevated in Id1-expressing cells, which may be, at least in part, responsible for the augmentation of their proliferation and survival. Taken together, results from this study suggest an important role of E and Id proteins in peripheral T cell activation. The ability of Id proteins to by-pass co-stimulatory signals to enable T cell activation has significant implications in regulating T cell immunity.

  2. Heavy-quark free energy at finite temperature with 2+1 flavors of improved Wilson quarks in fixed scale approach

    E-Print Network [OSTI]

    Y. Maezawa; S. Aoki; S. Ejiri; T. Hatsuda; K. Kanaya; H. Ohno; T. Umeda

    2009-11-02

    The free energy between a static quark and an antiquark is studied by using the color-singlet Polyakov-line correlation at finite temperature. We perform simulations on $32^3 \\times 12$, 10, 8, 6, 4 lattices in the high temperature phase with the RG-improved gluon action and 2+1 flavors of the clover-improved Wilson quark action. Since the simulations are based on the fixed scale approach that the temperature can be varied without changing the spatial volume and renormalization factor, it is possible to investigate temperature dependence of the heavy-quark free energy without any adjustment of the overall constant. We find that, the heavy-quark free energies at short distance converge to the heavy-quark potential evaluated from the Wilson-loop operator at zero temperature, in accordance with the expected insensitivity of short distance physics to the temperature. At long distance, the heavy-quark free energies approach to twice the single-quark free energies, implying that the interaction between heavy quarks is screened. The Debye screening mass obtained from the long range behavior of the heavy-quark free energy is compared with results of the thermal perturbation theory and those of $N_f=2$ and $N_f=0$ lattice simulations.

  3. The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells

    SciTech Connect (OSTI)

    Zhang, Yingyi; Zhao, Yu; Li, Leilei; Shen, Yu; Cai, Xiaoli; Zhang, Xiaodong; Ye, Lihong

    2013-05-03

    Highlights: •HBXIP is able to upregulate the expression of PDGFB in breast cancer cells. •HBXIP serves as a coactivator of activating transcription factor Sp1. •HBXIP stimulates the PDGFB promoter via activating transcription factor Sp1. •HBXIP promotes the proliferation of breast cancer cell via upregulating PDGFB. -- Abstract: We have reported that the oncoprotein hepatitis B virus X-interacting protein (HBXIP) acts as a novel transcriptional coactivator to promote proliferation and migration of breast cancer cells. Previously, we showed that HBXIP was able to activate nuclear factor-?B (NF-?B) in breast cancer cells. As an oncogene, the platelet-derived growth factor beta polypeptide (PDGFB) plays crucial roles in carcinogenesis. In the present study, we found that both HBXIP and PDGFB were highly expressed in breast cancer cell lines. Interestingly, HBXIP was able to increase transcriptional activity of NF-?B through PDGFB, suggesting that HBXIP is associated with PDGFB in the cells. Moreover, HBXIP was able to upregulate PDGFB at the levels of mRNA, protein and promoter in the cells. Then, we identified that HBXIP stimulated the promoter of PDGFB through activating transcription factor Sp1. In function, HBXIP enhanced the proliferation of breast cancer cells through PDGFB in vitro. Thus, we conclude that HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote proliferation of breast cancer cells.

  4. Ethanol increases matrix metalloproteinase-12 expression via NADPH oxidase-dependent ROS production in macrophages

    SciTech Connect (OSTI)

    Kim, Mi Jin; Nepal, Saroj; Lee, Eung-Seok; Jeong, Tae Cheon; Kim, Sang-Hyun; Park, Pil-Hoon

    2013-11-15

    Matrix metalloproteinase-12 (MMP-12), an enzyme responsible for degradation of extracellular matrix, plays an important role in the progression of various diseases, including inflammation and fibrosis. Although most of those are pathogenic conditions induced by ethanol ingestion, the effect of ethanol on MMP-12 has not been explored. In the present study, we investigated the effect of ethanol on MMP-12 expression and its potential mechanisms in macrophages. Here, we demonstrated that ethanol treatment increased MMP-12 expression in primary murine peritoneal macrophages and RAW 264.7 macrophages at both mRNA and protein levels. Ethanol treatment also significantly increased the activity of nicotinamide adenine dinucleotide (NADPH) oxidase and the expression of NADPH oxidase-2 (Nox2). Pretreatment with an anti-oxidant (N-acetyl cysteine) or a selective inhibitor of NADPH oxidase (diphenyleneiodonium chloride (DPI)) prevented ethanol-induced MMP-12 expression. Furthermore, knockdown of Nox2 by small interfering RNA (siRNA) prevented ethanol-induced ROS production and MMP-12 expression in RAW 264.7 macrophages, indicating a critical role for Nox2 in ethanol-induced intracellular ROS production and MMP-12 expression in macrophages. We also showed that ethanol-induced Nox2 expression was suppressed by transient transfection with dominant negative I?B-? plasmid or pretreatment with Bay 11-7082, a selective inhibitor of NF-?B, in RAW 264.7 macrophages. In addition, ethanol-induced Nox2 expression was also attenuated by treatment with a selective inhibitor of p38 MAPK, suggesting involvement of p38 MAPK/NF-?B pathway in ethanol-induced Nox2 expression. Taken together, these results demonstrate that ethanol treatment elicited increase in MMP-12 expression via increase in ROS production derived from Nox2 in macrophages. - Highlights: • Ethanol increases ROS production through up-regulation of Nox2 in macrophages. • Enhanced oxidative stress contributes to ethanol-induced MMP-12 expression. • p38 MAPK/NF-?B signaling pathway modulates ethanol-induced Nox2 expression.

  5. Anti-inflammatory and antifibrotic effects of methyl palmitate

    SciTech Connect (OSTI)

    El-Demerdash, Ebtehal, E-mail: ebtehal_dm@yahoo.com

    2011-08-01

    Methyl palmitate (MP) has been shown earlier to inhibit Kupffer cells and rat peritoneal macrophages. To evaluate the potential of MP to inhibit the activation of other macrophages, RAW cells (macrophages of alveolar origin) were treated with varying concentrations of MP (0.25, 0.5, 1 mM). Assessment of cytotoxicity using MTT assay revealed that 0.25 and 0.5 mM are not toxic to RAW cells. MP was able to inhibit the phagocytic function of RAW cells. Treatment of cells with MP 24 hours prior to LPS stimulation significantly decreased nitric oxide release and altered the pattern of cytokines release; there was a significant decrease in TNF-{alpha} and a significant increase in IL-10 compared to the controls. However, there is a non-significant change in IL-6 level. Furthermore, phosphorylation of inhibitory kappa B (I{kappa}B{alpha}) protein was significantly decreased in RAW cells treated with 0.5 mM MP after LPS stimulation. Based upon the in-vitro results, it was examined whether MP treatment will be effective in preventing bleomycin-induced lung inflammation and fibrosis in-vivo. Bleomycin given by itself caused destruction of the lung architecture characterized by pulmonary fibrosis with collapse of air alveoli and emphysematous. Bleomycin induced a significant increase in hydroxyproline level and activated NF-{kappa}B, p65 expression in the lung. MP co-treatment significantly ameliorated bleomycin effects. These results suggest that MP has a potential of inhibiting macrophages in general. The present study demonstrated for the first time that MP has anti-inflammatory and antifibrotic effect that could be through NF-kB inhibition. Thus MP like molecule could be a promising anti-inflammatory and antifibrotic drug. - Research Highlights: >Methyl palmitate is a universal macrophage inhibitor. >It could be a promising nucleus of anti-inflammatory and antifibrotic drugs. >The underlying mechanism of these effects could be through NF-kB inhibition.

  6. Detailed optical characterization of a near diffraction limited xenon fluoride laser

    SciTech Connect (OSTI)

    Londono, C. ); Smith, M.J.; Trainor, D.W.; Itzkan, I. ); Berggren, R. ); Fulghum, S.F. )

    1988-12-01

    A 1 m gain length, electron beam pumped xenon fluoride laser (lambda = 353, 351 nm) utilizing two laser mixtures of lean and rich NF/sub 3/, with Xe and balance Ne, was operated with a confocal unstable resonator with magnification of 2.24. The resultant beam quality was diagnosed with both shearing interferometry to measure near-field phase and far-field focal spot evaluation techniques. These measurements resulted in a beam quality of <1.15 times the diffraction limit with no evidence of the wide angle energy loss. This laser device was fully characterized with regard to electron beam deposition uniformity, transient refractive index effects, and optical quality of the resonator and diagnostic components.

  7. Performance of aluminide coatings applied on alloy CF8C plus at 800 C

    SciTech Connect (OSTI)

    Kumar, Deepak [ORNL] [ORNL; Dryepondt, Sebastien N [ORNL] [ORNL; Shyam, Amit [ORNL] [ORNL; Haynes, James A [ORNL] [ORNL; Pint, Bruce A [ORNL] [ORNL; Armstrong, Beth L [ORNL] [ORNL; Lara-Curzio, Edgar [ORNL] [ORNL

    2011-01-01

    The cost effective, austenitic stainless steel CF8C plus is an attractive alloy for massive cast structures such as steam turbine casings. The microstructure stability and creep strength of this alloy are better than commercial high-performance heat-resistant steels such as NF709 and Super 304H, and are comparable to the Ni-based superalloy Inconel 617. The oxidation resistance of the alloy in atmosphere rich in water vapor is however insufficient at T>800 C, and the use of diffusion aluminide coatings is considered for potential high temperature applications. The thermal stability and protectiveness of coatings applied on the CF8C plus substrate by pack cementation and slurry process were investigated in air + 10% H2O environment at 800 C. Further, the coating effect on the fatigue life of the alloy was assessed via low-cycle-fatigue experiments.

  8. RECENT PROGRESS TOWARD A MUON RECIRCULATING LINEAR ACCELERATOR

    SciTech Connect (OSTI)

    Slawomir Bogacz, Vasiliy Morozov, Yves Roblin, Kevin Beard

    2012-07-01

    Both Neutrino Factories (NF) and Muon Colliders (MC) require very rapid acceleration due to the short lifetime of muons. After a capture and bunching section, a linac raises the energy to about 900 MeV, and is followed by one or more Recirculating Linear Accelerators (RLA), possibly followed by a Rapid Cycling Synchnotron (RCS) or Fixed-Field Alternating Gradient (FFAG) ring. A RLA reuses the expensive RF linac section for a number of passes at the price of having to deal with different energies within the same linac. Various techniques including pulsed focusing quadruopoles, beta frequency beating, and multipass arcs have been investigated via simulations to improve the performance and reduce the cost of such RLAs.

  9. Measurement of the neutron F2 structure function via spectator tagging with CLAS

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Baillie, N.; Tkachenko, S.; Zhang, J.; Bosted, P.; Bültmann, S.; Christy, M. E.; Fenker, H.; Griffioen, K. A.; Keppel, C. E.; Kuhn, S. E.; et al

    2012-04-01

    We report on the first measurement of the F2 structure function of the neutron from semi-inclusive scattering of electrons from deuterium, with low-momentum protons detected in the backward hemisphere. Restricting the momentum of the spectator protons to ? 100 degrees relative to the momentum transfer allows an interpretation of the process in terms of scattering from nearly on-shell neutrons. The F2n data collected cover the nucleon resonance and deep-inelastic regions over a wide range of x for 0.65 2 2, with uncertainties from nuclear corrections estimated to be less thanmore »a few percent. These measurements provide the first determination of the neutron to proton structure function ratio F2n/F2p at 0.2 ?« less

  10. Low-energy Scattering of $(D^{*}\\bar{D}^{*})^\\pm$ System and the Resonance-like Structure $Z_c(4025)$

    E-Print Network [OSTI]

    Ying Chen; Ming Gong; Yu-Hong Lei; Ning Li; Jian Liang; Chuan Liu; Jin-Long Liu; Yong-Fu Liu; Yu-Bin Liu; Zhaofeng Liu; Jian-Ping Ma; Zhan-Lin Wang; Jian-Bo Zhang

    2015-03-09

    In this paper, low-energy scattering of the $(D^{*}\\bar{D}^{*})^\\pm$ meson system is studied within L\\"uscher's finite-size formalism using $N_{f}=2$ twisted mass gauge field configurations. With three different pion mass values, the $s$-wave threshold scattering parameters, namely the scattering length $a_0$ and the effective range $r_0$, are extracted in $J^P=1^+$ channel. Our results indicate that, in this particular channel, the interaction between the two vector charmed mesons is weakly repulsive in nature hence do not support the possibility of a shallow bound state for the two mesons, at least for the pion mass values being studied. This study provides some useful information on the nature of the newly discovered resonance-like structure $Z_c(4025)$ observed in various experiments.

  11. 100 Area D4 Project Building Completion Report - July 2007 to December 2008

    SciTech Connect (OSTI)

    M. T. Stankovich

    2009-04-15

    This report documents the decontamination, decommissioning, and demolition of the 105-NB, 163-N, 183-N, 183-NA, 183-NB, 183-NC, 184-N, 184-NA, 184-NB, 184-NC, 184-ND, 184-NE, 184-NF, 1312-N, 1330-N, 1705-N, 1705-NA, 1706-N, 1712-N, 1714-N, 1714-NA, 1714-NB, 1802-N, MO-050, MO-055, MO-358, MO-390, MO-900, MO-911, and MO-950 facilities in the 100 Area of the Hanford Site. The D4 activities for these facilities include utility disconnection, planning, characterization, engineering, removal of hazardous and radiological contaminated materials, equipment removal, decommissioning, deactivation, decontamination, demolition of the structure, and removal of the remaining slabs.

  12. Measurement of the neutron F2 structure function via spectator tagging with CLAS

    E-Print Network [OSTI]

    N. Baillie; S. Tkachenko; J. Zhang; P. Bosted; S. Bultmann; M. E. Christy; H. Fenker; K. A. Griffioen; C. E. Keppel; S. E. Kuhn; W. Melnitchouk; V. Tvaskis; K. P. Adhikari; D. Adikaram; M. Aghasyan; M. J. Amaryan; M. Anghinolfini; J. Arrington; H. Avakian; H. Baghdasaryan; M. Battaglieri; A. S. Biselli; 5 D. Branford; W. J. Briscoe; W. K. Brooks; V. D. Burkert; D. S. Carman; A. Celentano; S. Chandavar; G. Charles; P. L. Cole; M. Contalbrigo; V. Crede; A. D'Angelo; A. Daniel; N. Dashyan; R. De Vita; E. De Sanctis; A. Deur; B. Dey; C. Djalali; G. Dodge; J. Domingo; D. Doughty; R. Dupre; D. Dutta; R. Ent; H. Egiyan; A. El Alaoui; L. El Fassi; L. Elouadrhiri; P. Eugenio; G. Fedotov; S. Fegan; A. Fradi; M. Y. Gabrielyan; N. Gevorgyan; G. P. Gilfoyle; K. L. Giovanetti; F. X. Girod; W. Gohn; E. Golovatch; R. W. Gothe; L. Graham; B. Guegan; M. Guidal; N. Guler; L. Guo; K. Hafidi; D. Heddle; K. Hicks; M. Holtrop; E. Hungerford; C. E. Hyde; Y. Ilieva; D. G. Ireland; M. Ispiryan; E. L. Isupov; S. S. Jawalkar; H. S. Jo; N. Kalantarians; M. Khandaker; P. Khetarpal; A. Kim; W. Kim; P. M. King; A. Klein; F. J. Klein; A. Klimenko; V. Kubarovsky; S. V. Kuleshov; N. D. Kvaltine; K. Livingston; H. Y. Lu; I . J . D. MacGregor; Y. Mao; N. Markov; B. McKinnon; T. Mineeva; B. Morrison; H. Moutarde; E. Munevar; P. Nadel-Turonski; A. Ni; S. Niccolai; I. Niculescu; G. Niculescu; M. Osipenko; A. I. Ostrovidov; L. Pappalardo; K. Park; S. Park; E. Pasyuk; S. Anefalos Pereira; S. Pisano; S. Pozdniakov; J. W. Price; S. Procureur; Y. Prok; D. Protopopescu; B. A. Raue; G. Ricco; D. Rimal; M. Ripani; G. Rosner; P. Rossi; F. Sabatie; M. S. Saini; C. Salgado; D. Schott; R. A. Schumacher; E. Seder; Y. G. Sharabian; D. I. Sober; D. Sokhan; S. Stepanyan; S. S. Stepanyan; P. Stoler; S. Strauch; M. Taiuti; W. Tang; M. Ungaro; M. F. Vineyard; E. Voutier; D. P. Watts; L. B. Weinstein; D. P. Weygand; M. H. Wood; L. Zana; B. Zhao

    2012-05-14

    We report on the first measurement of the F2 structure function of the neutron from semi-inclusive scattering of electrons from deuterium, with low-momentum protons detected in the backward hemisphere. Restricting the momentum of the spectator protons to < 100 MeV and their angles to < 100 degrees relative to the momentum transfer allows an interpretation of the process in terms of scattering from nearly on-shell neutrons. The F2n data collected cover the nucleon resonance and deep-inelastic regions over a wide range of Bjorken x for 0.65 < Q2 < 4.52 GeV2, with uncertainties from nuclear corrections estimated to be less than a few percent. These measurements provide the first determination of the neutron to proton structure function ratio F2n/F2p at 0.2 < x < 0.8 with little uncertainty due to nuclear effects.

  13. Symanzik flow on HISQ ensembles

    E-Print Network [OSTI]

    The MILC Collaboration; A. Bazavov; C. Bernard; N. Brown; C. DeTar; J. Foley; Steven Gottlieb; U. M. Heller; J. E. Hetrick; J. Laiho; L. Levkova; M. Oktay; R. L. Sugar; D. Toussaint; R. S. Van de Water; R. Zhou

    2013-11-06

    We report on a scale determination with gradient-flow techniques on the $N_f = 2 + 1 + 1$ HISQ ensembles generated by the MILC collaboration. The lattice scale $w_0/a$, originally proposed by the BMW collaboration, is computed using Symanzik flow at four lattice spacings ranging from 0.15 to 0.06 fm. With a Taylor series ansatz, the results are simultaneously extrapolated to the continuum and interpolated to physical quark masses. We give a preliminary determination of the scale $w_0$ in physical units, along with associated systematic errors, and compare with results from other groups. We also present a first estimate of autocorrelation lengths as a function of flowtime for these ensembles.

  14. Wilson flow with naive staggered quarks

    E-Print Network [OSTI]

    Saumen Datta; Sourendu Gupta; Anirban Lahiri; Andrew Lytle; Pushan Majumdar

    2015-07-03

    Scale setting for QCD with two flavours of staggered quarks is examined using Wilson flow over a factor of four change in both the lattice spacing and the pion mass. The statistics needed to keep the errors in the flow scale fixed is found to increase approximately as the inverse square of the lattice spacing. Tree level improvement of the scales t_0 and w_0 is found to be useful in most of the range of lattice spacings we explore. The scale uncertainty due to remaining lattice spacing effects is found to be about 3%. The ratio w_0/\\sqrt{t_0} is N_f dependent and we find its continuum limit to be 1.106 \\pm 0.007 (stat) \\pm 0.005 (syst) for m_\\pi w_0 \\simeq 0.3.

  15. A study of programs in conservation and outdoor education in selected colleges and universities 

    E-Print Network [OSTI]

    Noel, Jean-Guy Claude

    1969-01-01

    is to analyze snia cc'bula&e t. i. e data gath red chro:igh the use of;- o; stioi naire o=t sining to che nature of existing programs ". f conservation and outdoor cd;. -;tion in selected col)ages nd/or universities, t' he user catesories u outdoor la... made this course of stud; po!. ib!. e. . mNEI OF CON 1!NTS v ge NR SIR Ai)T t IST !IP Ti RI i)S v- . I Cn iiIi L e z OR' TNTATI in". I TO TI!F, STIJDY ?11 ti ui Uctroil Stal eicent o" che Problem &i vpc-e nf t!is vtui)y LI tii ? ' o'ls e...

  16. Light Meson Distribution Amplitudes

    E-Print Network [OSTI]

    R. Arthur; P. A. Boyle; D. Brömmel; M. A. Donnellan; J. M. Flynn; A. Jüttner; H. Pedroso de Lima; T. D. Rae; C. T. Sachrajda; B. Samways

    2010-11-12

    We calculated the first two moments of the light-cone distribution amplitudes for the pseudoscalar mesons ($\\pi$ and $K$) and the longitudinally polarised vector mesons ($\\rho$, $K^*$ and $\\phi$) as part of the UKQCD and RBC collaborations' $N_f=2+1$ domain-wall fermion phenomenology programme. These quantities were obtained with a good precision and, in particular, the expected effects of $SU(3)$-flavour symmetry breaking were observed. Operators were renormalised non-perturbatively and extrapolations to the physical point were made, guided by leading order chiral perturbation theory. The main results presented are for two volumes, $16^3\\times 32$ and $24^3\\times 64$, with a common lattice spacing. Preliminary results for a lattice with a finer lattice spacing, $32^3\\times64$, are discussed and a first look is taken at the use of twisted boundary conditions to extract distribution amplitudes.

  17. Seasonal abundance, distribution and growth of commercially important marine crustaceans at a hot water discharge in Galveston Bay, Texas 

    E-Print Network [OSTI]

    Gallaway, B. J

    1970-01-01

    Ig tl JJ 0 IC Ct cd u ?0 Cl Cl CO t? 0 IC U W IO W 'R Iel 0 A NOlof f-tc?ttcr OINr CONOCI tcwelr ultct&OI N N N f ONNOlOelNNullelf Nf O?ecelOCOIOtcul& N N N QQHtOO+elONCOQ?ef OCI ut&OHI ONN ?fNN N I o N o ul + p ul ot ut Ifl ul ct & 0..., 1968 to a high of 25. 5 o/oo on the bottom at offshore stati. on 11 in February of 1969. The water was most saline during winter periods (Figure 3). Winter peaks were followed by a sharp drop in sa- linity associated with spring rains and resulting...

  18. Experimental wrench faulting at confining pressure 

    E-Print Network [OSTI]

    Bartlett, Wendy Louise

    1980-01-01

    0 )J S S S- Vl Itf th Ql S J= C Vl C s 0 I ? C Cl 'l3 GJ IV CI Cl 0 0 Cl )O C C Ql QJ th Vl 5- S- GI Ql Gl Gl C C QJ Gl CI Ql S JC '0 U nf 0 0 0 Vl th Ih Vl QJ S S S 5 )I )) )) QJ V)0'UUS 5- 0 0 0 C SEEES S 5- 5 5- C S GJ IV SJ... the HP9825A program for data re- duction; Larry Casarta, for participating in da1ly discuss1ons about experimental design, stress, stra1n, and natural shear zones; Lenora [roodler, Marjle White, and Arthur F. X. O'Keefe, for enlightening conversations...

  19. Electrostatic potential variation on the flux surface and its impact on impurity transport

    E-Print Network [OSTI]

    García-Regaña, J M; Turkin, Y; Kleiber, R; Helander, P; Maaßberg, H; Alonso, J A; Velasco, J L

    2015-01-01

    The particle transport of impurities in magnetically confined plasmas under some conditions does not find, neither quantitatively nor qualitatively, a satisfactory theory-based explanation. This compromise the successful realization of thermo-nuclear fusion for energy production since its accumulation is known to be one of the causes that leads to the plasma breakdown. In standard reactor-relevant conditions this accumulation is in most stellarators intrinsic to the lack of toroidal symmetry, that leads to the neoclassical electric field to point radially inwards. This statement, that the standard theory allows to formulate, has been contradicted by some experiments that showed weaker or no accumulation under such conditions \\cite{Ida_pop_16_056111_2009, Yoshinuma_nf_49_062002_2009}. The charge state of the impurities makes its transport more sensitive to the electric fields. Thus, the short length scale turbulent electrostatic potential or its long wave-length variation on the flux surface $\\Phi_{1}$ -- that...

  20. Injection/Extraction Studies for the Muon FFAG

    SciTech Connect (OSTI)

    Pasternak, J.; Berg, J. Scott; Kelliher, D. J.; Machida, S.

    2010-03-30

    The non-scaling fixed field alternating gradient (NS-FFAG) ring is a candidate muon accelerator in the Neutrino Factory complex according to the present baseline, which is currently being addressed by the International Design Study (IDS-NF). In order to achieve small orbit excursion, motivated by magnet cost reduction, and small time of flight variation, dictated by the need to use high RF frequency, lattices with a very compact cell structure and short straight sections are required. The resulting geometry dictates very difficult constraints on the injection/extraction systems. Beam dynamics in the non-scaling FFAG is studied using codes capable of correctly tracking with large transverse amplitude and momentum spread. The feasibility of injection/extraction is studied and various implementations focusing on minimization of kicker/septum strength are presented. Finally the parameters of the resulting kicker magnets are estimated.

  1. Factors Influencing Runoff and Soil Erosion. 

    E-Print Network [OSTI]

    Conner, A. B. (Arthur Benjamin)

    1930-01-01

    for the three years occurred in July and August, 1926, I COO A00 $ 3.00 $ % * P.00 1.00 0.00 I* llrNf/;Y I-P c-.v n /-ro a-lops I.- ,,-#a- M N-~CI-Y ~~cID~cx/-@~~ ,a II.~O?+S /-w btoam +a N-ZO~~YI I+N-~~IMM/CWWI Jon. hFe6. MOC &r h%y Jon. Ju,! Auy. +f.... F. DANA M. i. ~laAt Pathologist Veterrnnrran H. E. RE*. B. s., Agronomist; Cotton ~oot ~ot **:: E: ~:~~~~~~~-~Shl~f$mO1OgiSt Invesf igations SIMON E. WOLFF, M. S., Botanist; Cotton Root N~~~~~,"~ ~id{'g~~~f~k:ndent Rot Investigations SHERMAN...

  2. A Markov method for generating capacity reliability evaluation including operating considerations 

    E-Print Network [OSTI]

    Asgarpoor, Sohrab

    1981-01-01

    =NN 00 61 1=1 N OQ 61 J= I N E( I J)=O CONTINUE KG=0 00 30 KK I NLL (F(KK NE I) GO TO 31 GQ TO 32 IF(I OAO&KK) LT LOAD(KK-I IF(LOAD(KK) GT ~ LQAD(KX-I IF&KG GT ~ OI GO TQ 44 CQ TQ 32 N=NN 00 36 J I N SD & KK ~ J) =58& KK-I J )-SB (K CONTINUE... N=NN UNITS WHICH HAS FAILED IN CLASS ) I 6), IF& J& ~ V(J) 5X, I5) &) 0 70 33 )) GQ TQ 32 K+I ~ J) 33 CALL MOOST &A ~ IF D V CAP ~ 4EW 4 LQAD LAM Ul?N NC, N KK ~ ALPHA ~ ST 1 CAPP KL NT I NF, CAPS ~ KAPS 53 SD 'VR NU PS, TS DELTS) 84 85 86...

  3. Fiber-Base Duality and Global Symmetry Enhancement

    E-Print Network [OSTI]

    Vladimir Mitev; Elli Pomoni; Masato Taki; Futoshi Yagi

    2014-11-10

    We show that the 5D Nekrasov partition functions enjoy the enhanced global symmetry of the UV fixed point. The fiber-base duality is responsible for the global symmetry enhancement. For $SU(2)$ with $N_f\\leq 7$ flavors the fiber-base symmetry together with the manifest flavor $SO(2N_f)$ symmetry generate the $E_{N_f+1}$ global symmetry, while in the higher rank case the manifest global symmetry of the two dual theories related by the fiber-base duality map generate the symmetry enhancement. The symmetry enhancement at the level of the partition function is manifest once we chose an appropriate reparametrization for the Coulomb moduli.

  4. Method for studying a sample of material using a heavy ion induced mass spectrometer source

    DOE Patents [OSTI]

    Fries, David P. (St. Petersburg, FL); Browning, James F. (Palm Harbour, FL)

    1999-01-01

    A heavy ion generator is used with a plasma desorption mass spectrometer to provide an appropriate neutron flux in the direction of a fissionable material in order to desorb and ionize large molecules from the material for mass analysis. The heavy ion generator comprises a fissionable material having a high n,f reaction cross section. The heavy ion generator also comprises a pulsed neutron generator that is used to bombard the fissionable material with pulses of neutrons, thereby causing heavy ions to be emitted from the fissionable material. These heavy ions impinge on a material, thereby causing ions to desorb off that material. The ions desorbed off the material pass through a time-of-flight mass analyzer, wherein ions can be measured with masses greater than 25,000 amu.

  5. Studies on the existence of a translocatable florigen antagonist in Xanthium pensylvanicum Wallr. and Glycine max (L.) Merr. V. Biloxi 

    E-Print Network [OSTI]

    Brown, Oswald David Robert

    1960-01-01

    to support the second of the postulated flowering theories i. e. the ~ florigen versus a translocatablo florigen antagonist~ theory. For only 43 ' 18/ of the nodes of the branches in Commi rtment 4 showed indications of flowering as against 78. 5'jg... i;g 1 ". , 3? 0 7 0 8 0 5 0 6 0 5 0 0 4 0 5 0 0 4. 0 5 0 5 0 6 0 4 0 6 0 7 0 0 0 5 0 b Long Days Compartment, 2 Control Test F N F F N F Short Day Compartment, 3 Contx ol Test F M-F F N-F 0 0 7 0 5 0 7 0 0 5 2 0 2 4 0 0 5...

  6. QCD equation of state at nonzero chemical potential: continuum results with physical quark masses at order mu^2

    E-Print Network [OSTI]

    Sz. Borsanyi; G. Endrodi; Z. Fodor; S. D. Katz; S. Krieg; C. Ratti; K. K. Szabo

    2012-08-08

    We determine the equation of state of QCD for nonzero chemical potentials via a Taylor expansion of the pressure. The results are obtained for N_f=2+1 flavors of quarks with physical masses, on various lattice spacings. We present results for the pressure, interaction measure, energy density, entropy density, and the speed of sound for small chemical potentials. At low temperatures we compare our results with the Hadron Resonance Gas model. We also express our observables along trajectories of constant entropy over particle number. A simple parameterization is given (the Matlab/Octave script parameterization.m, submitted to the arXiv along with the paper), which can be used to reconstruct the observables as functions of T and mu, or as functions of T and S/N.

  7. Multi-Step stochastic correction in dynamical fermion updating algorithms

    E-Print Network [OSTI]

    Enno E. Scholz; Istvan Montvay

    2006-10-20

    The advantages of using Multi-Step corrections for simulations of lattice gauge theories with dynamical fermions will be discussed. This technique is suited for algorithms based on the Multi-Boson representation of the dynamical fermions as well as for the Hybrid Monte-Carlo (HMC) algorithm and variants of the latter, like the Polynomial-HMC. Especially the latter has the power to deal with an odd number of fermion fields--an essential feature necessary for realistic QCD-simulations with up-, down-, and strange-quarks. In particular, we will discuss the application of the multi-step (actually two-step) correction technique to a PHMC updating algorithm for twisted-mass Wilson fermions with non-degenerate fermion masses, as it was used in recent dynamical simulations for N_f=2+1+1 fermion flavors.

  8. Engineering peptides to promote stabilizing interactions in the solid state

    E-Print Network [OSTI]

    Sinha, Sandipan; Thompson, S.; Camarda, K.; Topp, E.

    2006-10-25

    py rro lid on e ( PV P) an d pe pt ide ty ro sin e r es id ue s c an st ab iliz e p ep tid es ag ain st de am id ati on in th e s oli d sta te2 . U nf or tu na tel y, un de sir ab le co va len t r ea cti on s w ere al so ob se rv ed 3... la bil e (A sn ), an d th e po ssi bil ity o f inc lu di ng m ult ipl e sta bil izi ng r es id ue s. W e hy po th es ize t ha t no n- co va len t int era cti on s be tw ee n pe pt ide s a nd PV P i n s olu tio n m ay ar ise...

  9. Four-flavour leading-order hadronic contribution to the muon anomalous magnetic moment

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Burger, Florian; Feng, Xu; Hotzel, Grit; Jansen, Karl; Univ. of Cyprus, Nicosia; Petschlies, Marcus; Renner, Dru B.

    2014-02-24

    We present a four-flavour lattice calculation of the leading-order hadronic vacuum polarisation contribution to the anomalous magnetic moment of the muon, a?hvp, arising from quark-connected Feynman graphs. It is based on ensembles featuring Nf=2+1+1 dynamical twisted mass fermions generated by the European Twisted Mass Collaboration (ETMC). Several light quark masses are used in order to yield a controlled extrapolation to the physical pion mass. We employ three lattice spacings to examine lattice artefacts and several different volumes to check for finite-size effects. Including the complete first two generations of quarks allows for a direct comparison with phenomenological determinations of amore »?hvp. The final result involving an estimate of the systematic uncertainty a?hvp=6.74 (21)(18) 10-8 shows a good overall agreement with these computations.« less

  10. Applications of Game Theory to Multi-Agent Coordination Problems in Communication Networks 

    E-Print Network [OSTI]

    Ramaswamy Pillai, Vinod

    2013-08-28

    the assumption is that the content has not yet been released, and therefore is not yet available in the illicit P2P swarm. From this initial condition, Ni(0) evolves as follows: dNi(t) dt = min { ?(t) ( Nw(t) + dI(t) dt ) , Nr(t) +Nf (t) } , (2.8....5) and (2.6). From (2.8), the illegal growth rate is dNi(t) dt (a)= ?(t)Nw(t) (2.19) (b)= (?Nl(t)+?Ni(t))(N?(Nl(t)+Ni(t)))N . (2.20) (a) follows from the definition of ?(t) and the fact that Nw(t) ? N . (b) follows from (2.18) 16 and (2.4). From equation (2...

  11. SULFUR HEXAFLUORIDE TREATMENT OF USED NUCLEAR FUEL TO ENHANCE SEPARATIONS

    SciTech Connect (OSTI)

    Gray, J.; Torres, R.; Korinko, P.; Martinez-Rodriguez, M.; Becnel, J.; Garcia-Diaz, B.; Adams, T.

    2012-09-25

    Reactive Gas Recycling (RGR) technology development has been initiated at Savannah River National Laboratory (SRNL), with a stretch-goal to develop a fully dry recycling technology for Used Nuclear Fuel (UNF). This approach is attractive due to the potential of targeted gas-phase treatment steps to reduce footprint and secondary waste volumes associated with separations relying primarily on traditional technologies, so long as the fluorinators employed in the reaction are recycled for use in the reactors or are optimized for conversion of fluorinator reactant. The developed fluorination via SF{sub 6}, similar to the case for other fluorinators such as NF{sub 3}, can be used to address multiple fuel forms and downstream cycles including continued processing for LWR via fluorination or incorporation into a aqueous process (e.g. modified FLUOREX) or for subsequent pyro treatment to be used in advanced gas reactor designs such metal- or gas-cooled reactors. This report details the most recent experimental results on the reaction of SF{sub 6} with various fission product surrogate materials in the form of oxides and metals, including uranium oxides using a high-temperature DTA apparatus capable of temperatures in excess of 1000{deg}C . The experimental results indicate that the majority of the fission products form stable solid fluorides and sulfides, while a subset of the fission products form volatile fluorides such as molybdenum fluoride and niobium fluoride, as predicted thermodynamically. Additional kinetic analysis has been performed on additional fission products. A key result is the verification that SF{sub 6} requires high temperatures for direct fluorination and subsequent volatilization of uranium oxides to UF{sub 6}, and thus is well positioned as a head-end treatment for other separations technologies, such as the volatilization of uranium oxide by NF{sub 3} as reported by colleagues at PNNL, advanced pyrochemical separations or traditional full recycle approaches. Based on current results of the research at SRNL on SF{sub 6} fluoride volatility for UNF separations, SF{sub 6} treatment renders all anticipated volatile fluorides studied to be volatile, and all non-volatile fluorides studied to be non-volatile, with the notable exception of uranium oxides. This offers an excellent opportunity to use this as a head-end separations treatment process because: 1. SF{sub 6} can be used to remove volatile fluorides from a UNF matrix while leaving behind uranium oxides. Therefore an agent such as NF{sub 3} should be able to very cleanly separate a pure UF{sub 6} stream, leaving compounds in the bottoms such as PuF{sub 4}, SrF{sub 2} and CsF after the UNF matrix has been pre-treated with SF{sub 6}. 2. Due to the fact that the uranium oxide is not separated in the volatilization step upon direct contact with SF{sub 6} at moderately high temperatures (? 1000{deg}C), this fluoride approach may be wellsuited for head-end processing for Gen IV reactor designs where the LWR is treated as a fuel stock, and it is not desired to separate the uranium from plutonium, but it is desired to separate many of the volatile fission products. 3. It is likely that removal of the volatile fission products from the uranium oxide should simplify both traditional and next generation pyroprocessing techniques. 4. SF{sub 6} treatment to remove volatile fission products, with or without treatment with additional fluorinators, could be used to simplify the separations of traditional aqueous processes in similar fashion to the FLUOREX process. Further research should be conducted to determine the separations efficiency of a combined SF{sub 6}/NF{sub 3} separations approach which could be used as a stand-alone separations technology or a head-end process.

  12. Analysis and Annotation of Nucleic Acid Sequence

    SciTech Connect (OSTI)

    States, David J.

    2004-07-28

    The aims of this project were to develop improved methods for computational genome annotation and to apply these methods to improve the annotation of genomic sequence data with a specific focus on human genome sequencing. The project resulted in a substantial body of published work. Notable contributions of this project were the identification of basecalling and lane tracking as error processes in genome sequencing and contributions to improved methods for these steps in genome sequencing. This technology improved the accuracy and throughput of genome sequence analysis. Probabilistic methods for physical map construction were developed. Improved methods for sequence alignment, alternative splicing analysis, promoter identification and NF kappa B response gene prediction were also developed.

  13. The design of efficient input circuits for class C amplifiers 

    E-Print Network [OSTI]

    Fristoe, Harold T.

    1953-01-01

    ?? ?? ?? ?????? ?? ? , - ^ z O ^ Q z) o o?? l ?g? ?8? ??e?s g?s n?o?s nfn??ded ed ?nds? ??of ?of?sfgeofn? ?sde?f ?sg?o?d? eg ed n?do ef?oi?nge?s go ? ? ? ? ?fr? je??is ??X rs?sids ?offs?geof o? g?s m je?gsi ?? ?ofde?si nf n?gsifngs do??geof o? g?s fsg?oi?X tf s... ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? c ? ? ? c ? ? n ? ? o g ? ? ? c c o ? ? c ? ? ? ? ? o n ? n X ? ? o ? ? g ? ? ? ? ? ? ? ? ? ? o ? a ? ? d ? ? ? X m c ? m ? ? ? u ? ? 1 . 8 ? ? ? ? ? ? ? c X ? ? ? ? ? ?n ? ? n ? g g g c ? f ? ? ? X ? m ? ? ? ? ? m ? ? ? ? X X n ? 2 a a...

  14. Nepali Aawaz Volume 1, Issue 11, 8 March 2006

    E-Print Network [OSTI]

    Shrestha, Kashish Das

    FOR DETAILS AND IMMEDIATE WORK OPPORTUNITIES: 136- 89 ROOSEVELT AVE. #303, FLUSHING, NY 11354 PHONE: 718- 762- 4001 7Nepali Aawaz | Falgun 25.2062 Politics 6f9f /x]sfn] cfGbf]ngn] lng' kg]{ htL ultL lng g;s]sf] o'jf g]tf uug yfkf atfp5g . k|:t't 5, nf]s tfl... Gg] sfdgf ug'{ Aoy{ x'G5 . pgLx?sf] s'/f ;'Gg' k5{ / xfd|f] s'/f a'‰fpg'k5{ . clxn] eO{/x]sf] cfGbf]ng klg o'jfx?n] g} l6sfO{ /x]sf5g, c‰ of] lgl:qmo ;d'xnfO{ ;lqmo kfg]{ xf] eg] s:tf] utL lnG5 lnG5 . clxn] klg cf...

  15. Ogmios 11

    E-Print Network [OSTI]

    Ostler, Nicholas D M

    1999-01-01

    l id Bee Na'nf 'd T& bee nAstYahd66 nahAaG T "T6 kg"' t'66y6 nihi* dahoonesdo Mts' 6zigo hahazt'i' lei' d$$' hadahaana' NihidGhg66 ei t6 n

  16. On the efficiency of stochastic volume sources for the determination of light meson masses

    E-Print Network [OSTI]

    E. Endress; A. Jüttner; H. Wittig

    2011-11-25

    We investigate the efficiency of single timeslice stochastic sources for the calculation of light meson masses on the lattice as one varies the quark mass. Simulations are carried out with Nf = 2 flavours of non-perturbatively O(a) improved Wilson fermions for pion masses in the range of 450 - 760 MeV. Results for pseudoscalar and vector meson two-point correlation functions computed using stochastic as well as point sources are presented and compared. At fixed computational cost the stochastic approach reduces the variance considerably in the pseudoscalar channel for all simulated quark masses. The vector channel is more affected by the intrinsic stochastic noise. In order to obtain stable estimates of the statistical errors and a more pronounced plateau for the effective vector meson mass, a relatively large number of stochastic sources must be used.

  17. A fully-integrated aptamer-based affinity assay platform for monitoring astronaut health in space.

    SciTech Connect (OSTI)

    Yang, Xianbin (AM Biotechnologies LLC, Houston, TX); Durland, Ross H. (AM Biotechnologies LLC, Houston, TX); Hecht, Ariel H.; Singh, Anup K.; Sommer, Gregory Jon; Hatch, Anson V.

    2010-07-01

    Here we demonstrate the suitability of robust nucleic acid affinity reagents in an integrated point-of-care diagnostic platform for monitoring proteomic biomarkers indicative of astronaut health in spaceflight applications. A model thioaptamer targeting nuclear factor-kappa B (NF-{kappa}B) is evaluated in an on-chip electrophoretic gel-shift assay for human serum. Key steps of (i) mixing sample with the aptamer, (ii) buffer exchange, and (iii) preconcentration of sample were successfully integrated upstream of fluorescence-based detection. Challenges due to (i) nonspecific interactions with serum, and (ii) preconcentration at a nanoporous membrane are discussed and successfully resolved to yield a robust, rapid, and fully-integrated diagnostic system.

  18. Use of Treated Municipal Wastewater as Power Plant Cooling System Makeup Water: Tertiary Treatment versus Expanded Chemical Regimen for Recirculating Water Quality Management

    SciTech Connect (OSTI)

    David Dzombak; Radisav Vidic; Amy Landis

    2012-06-30

    Treated municipal wastewater is a common, widely available alternative source of cooling water for thermoelectric power plants across the U.S. However, the biodegradable organic matter, ammonia-nitrogen, carbonate and phosphates in the treated wastewater pose challenges with respect to enhanced biofouling, corrosion, and scaling, respectively. The overall objective of this study was to evaluate the benefits and life cycle costs of implementing tertiary treatment of secondary treated municipal wastewater prior to use in recirculating cooling systems. The study comprised bench- and pilot-scale experimental studies with three different tertiary treated municipal wastewaters, and life cycle costing and environmental analyses of various tertiary treatment schemes. Sustainability factors and metrics for reuse of treated wastewater in power plant cooling systems were also evaluated. The three tertiary treated wastewaters studied were: secondary treated municipal wastewater subjected to acid addition for pH control (MWW_pH); secondary treated municipal wastewater subjected to nitrification and sand filtration (MWW_NF); and secondary treated municipal wastewater subjected nitrification, sand filtration, and GAC adsorption (MWW_NFG). Tertiary treatment was determined to be essential to achieve appropriate corrosion, scaling, and biofouling control for use of secondary treated municipal wastewater in power plant cooling systems. The ability to control scaling, in particular, was found to be significantly enhanced with tertiary treated wastewater compared to secondary treated wastewater. MWW_pH treated water (adjustment to pH 7.8) was effective in reducing scale formation, but increased corrosion and the amount of biocide required to achieve appropriate biofouling control. Corrosion could be adequately controlled with tolytriazole addition (4-5 ppm TTA), however, which was the case for all of the tertiary treated waters. For MWW_NF treated water, the removal of ammonia by nitrification helped to reduce the corrosivity and biocide demand. Also, the lower pH and alkalinity resulting from nitrification reduced the scaling to an acceptable level, without the addition of anti-scalant chemicals. Additional GAC adsorption treatment, MWW_NFG, yielded no net benefit. Removal of organic matter resulted in pitting corrosion in copper and cupronickel alloys. Negligible improvement was observed in scaling control and biofouling control. For all of the tertiary treatments, biofouling control was achievable, and most effectively with pre-formed monochloramine (2-3 ppm) in comparison with NaOCl and ClO2. Life cycle cost (LCC) analyses were performed for the tertiary treatment systems studied experimentally and for several other treatment options. A public domain conceptual costing tool (LC3 model) was developed for this purpose. MWW_SF (lime softening and sand filtration) and MWW_NF were the most cost-effective treatment options among the tertiary treatment alternatives considered because of the higher effluent quality with moderate infrastructure costs and the relatively low doses of conditioning chemicals required. Life cycle inventory (LCI) analysis along with integration of external costs of emissions with direct costs was performed to evaluate relative emissions to the environment and external costs associated with construction and operation of tertiary treatment alternatives. Integrated LCI and LCC analysis indicated that three-tiered treatment alternatives such as MWW_NSF and MWW_NFG, with regular chemical addition for treatment and conditioning and/or regeneration, tend to increase the impact costs and in turn the overall costs of tertiary treatment. River water supply and MWW_F alternatives with a single step of tertiary treatment were associated with lower impact costs, but the contribution of impact costs to overall annual costs was higher than all other treatment alternatives. MWW_NF and MWW_SF alternatives exhibited moderate external impact costs with moderate infrastructure and chemical conditioner dosing, which makes them (especially

  19. Method for studying a sample of material using a heavy ion induced mass spectrometer source

    DOE Patents [OSTI]

    Fries, D.P.; Browning, J.F.

    1999-02-16

    A heavy ion generator is used with a plasma desorption mass spectrometer to provide an appropriate neutron flux in the direction of a fissionable material in order to desorb and ionize large molecules from the material for mass analysis. The heavy ion generator comprises a fissionable material having a high n,f reaction cross section. The heavy ion generator also comprises a pulsed neutron generator that is used to bombard the fissionable material with pulses of neutrons, thereby causing heavy ions to be emitted from the fissionable material. These heavy ions impinge on a material, thereby causing ions to desorb off that material. The ions desorbed off the material pass through a time-of-flight mass analyzer, wherein ions can be measured with masses greater than 25,000 amu. 3 figs.

  20. Nuclear forces in the parity odd sector and the LS forces

    E-Print Network [OSTI]

    Keiko Murano; for the HALQCD Collaboration

    2011-12-09

    In this paper, we report our first attempt at determining NN potentials in the parity odd sector including the spin-orbit force in lattice QCD, employing the method to extract successfully parity even NN potentials from Nambu-Bethe-Salpeter (NBS) wave functions through the Schr\\"odinger equation. Using Nf = 2 CP-PACS gauge configurations on a 16^3 x 32 lattice at a = 0.16 fm and m_\\pi \\cong 1.1 GeV, we calculate central, tensor and spin-orbit potentials in the parity odd sector. Although statistical errors are still large, we observe that the qualitative features of these potentials roughly agree with those of phenomenological potentials.

  1. XDATAP

    SciTech Connect (OSTI)

    Fisher, H.M.

    1988-03-01

    XDATAP is a device-independent, versatile, interactive, graphics utility whose esential function is the plotting of cross-section data. The code is based on DISSPLA Graphics and may also be run using a command file. While the original intent of the code was to compare cross sections in A Compact ENDF format with ENDF Evaluated Nuclear Data Files, presently the code is useful as a data diagnostic tool, that is, for the review, examination, comparison, and plotting of cross-section data. The code is also very useful as a general plotting utility for those users interested in examining other types of data. XDATAP is capable of representing a variety of reaction types of MTs, for example, total, elastic scattering, (n,2n), (n,en), (n,f), heating data, nubar data, producing pie diagrams, color plots, etc., manipulating data in several different formats contained on a variety of Nuclear Data Libraries (MCNP, ENDF, ENDL, Random Multigrooup, etc).

  2. Kaon semileptonic decay form factors with HISQ valence quarks

    E-Print Network [OSTI]

    E. Gamiz; J. A. Bailey; A. Bazavov; C. Bernard; C. Bouchard; C. DeTar; D. Du; A. X. El-Khadra; J. Foley; E. D. Freeland; Steven Gottlieb; U. M. Heller; J. Kim; A. S. Kronfeld; J. Laiho; L. Levkova; P. B. Mackenzie; E. T. Neil; M. B. Oktay; Si-Wei Qiu; J. N. Simone; R. Sugar; D. Toussaint; R. S. Van de Water; R. Zhou

    2012-11-05

    We report on the status of our kaon semileptonic form factor calculations using the highly-improved staggered quark (HISQ) formulation to simulate the valence fermions. We present results for the form factor f_+^{K \\pi}(0) on the asqtad N_f=2+1 MILC configurations, discuss the chiral-continuum extrapolation, and give a preliminary estimate of the total error. We also present a more preliminary set of results for the same form factor but with the sea quarks also simulated with the HISQ action; these results include data at the physical light quark masses. The improvements that we expect to achieve with the use of the HISQ configurations and simulations at the physical quark masses are briefly discussed.

  3. The $D_s$, $D^+$, $B_s$ and $B$ decay constants from $2+1$ flavor lattice QCD

    E-Print Network [OSTI]

    A. Bazavov; C. Bernard; C. Bouchard; C. DeTar; D. Du; A. X. El-Khadra; J. Foley; E. D. Freeland; E. Gámiz; Steven Gottlieb; U. M. Heller; J. Kim; J. Komijani; A. S. Kronfeld; J. Laiho; L. Levkova; P. B. Mackenzie; D. Mohler; E. T. Neil; M. B. Oktay; S. Qiu; J. N. Simone; R. L. Sugar; D. Toussaint; R. S. Van de Water; R. Zhou

    2014-03-27

    We present a study of the $D$ and $B$ leptonic decay constants on the MILC $N_f=2+1$ asqtad gauge ensembles using asqtad-improved staggered light quarks and clover heavy quarks in the Fermilab interpretation. Our previous analysis \\cite{Bazavov:2011aa} computed the decay constants at lattice spacings $a \\approx 0.14, 0.11$ and $0.083$ fm. We have extended the simulations to finer $a \\approx 0.058$ and $0.043$ fm lattice spacings, and have also increased statistics; this allows us to address many important sources of uncertainty. Technical advances include a two-step two-point fit procedure, better tuning of the heavy quark masses and a better determination of the axial-vector current matching. The present analysis remains blinded, so here we focus on the improvements and their predicted impact on the error budget compared to the prior analysis.

  4. Matrix Elements for $D$- and $B$-Mixing from 2+1 Flavor Lattice QCD

    E-Print Network [OSTI]

    C. C. Chang; C. Bernard; C. M. Bouchard; A. X. El-Khadra; E. D. Freeland; E. Gámiz; A. S. Kronfeld; J. Laiho; R. S. van de Water

    2013-11-26

    We present the status of our calculation of hadronic matrix elements for $D$- and $B$-meson mixing. We use a large set of the MILC collaboration's $N_f=2+1$ asqtad ensembles, which includes lattice spacings in the range $a\\approx0.12$-0.045 fm, and up/down to strange quark mass ratios as low as 0.05. The asqtad action is also employed for the light valence quarks. For the heavy quarks we use the Sheikholeslami-Wohlert action with the Fermilab interpretation. Our calculation covers the complete set of five local operators needed to describe $B$-meson mixing in the Standard Model and Beyond. In the charm sector, our calculation of local mixing matrix elements may be used to constrain new physics models. We present final correlator fit results on the full data set for the $B$-meson mixing project and preliminary fit results for the $D$-meson mixing project.

  5. Nucleon form factors with 2+1 flavor dynamical domain-wall fermions

    E-Print Network [OSTI]

    Takeshi Yamazaki; Yasumichi Aoki; Tom Blum; Huey-Wen Lin; Shigemi Ohta; Shoichi Sasaki; Robert Tweedie; James Zanotti

    2009-04-14

    We report our numerical lattice QCD calculations of the isovector nucleon form factors for the vector and axialvector currents: the vector, induced tensor, axialvector, and induced pseudoscalar form factors. The calculation is carried out with the gauge configurations generated with N_f=2+1 dynamical domain wall fermions and Iwasaki gauge actions at beta = 2.13, corresponding to a cutoff 1/a = 1.73 GeV, and a spatial volume of (2.7 fm)^3. The up and down quark masses are varied so the pion mass lies between 0.33 and 0.67 GeV while the strange quark mass is about 12% heavier than the physical one. We calculate the form factors in the range of momentum transfers, 0.2 6 is required to ensure that finite volume effects are below 1%.

  6. Triton Electric Form Factor at Low-Energies

    E-Print Network [OSTI]

    H. Sadeghi

    2009-08-14

    Making use of the Effective Field Theory(EFT) expansion recently developed by the authors, we compute the charge form factor of triton up to next-to-next-to-leading order (N$^2$LO). The three-nucleon forces(3NF) is required for renormalization of the three-nucleon system and it effects are predicted for process and is qualitatively supported by available experimental data. We also show that, by including higher order corrections, the calculated charge form factor and charge radius of $^3$H are in satisfactory agreement with the experimental data and the realistic Argonne $v_{18}$ two-nucleon and Urbana IX potential models calculations. This method makes possible a high precision few-body calculations in nuclear physics. Our result converges order by order in low energy expansion and also cut-off independent.

  7. Studies on the amino acid requirements of horses 

    E-Print Network [OSTI]

    Word, James Dewey

    1968-01-01

    in nzking digestib 1 il messur. . ? nw nts . R conf & y new ts ci;nf u. . ", hav ' b:r c ", s; ec . Ror creme] 4 inh L?!ii 1 iiy a iu grain portions of rice !io se rarrio i inl'o a si ngl? nc . "lr r wo?1r! hrilp assurri mrore occurs! e cn& n&tg 1.... l her cxLI Bc? was Itn&i i'i r: gr!8 1 &lc lr& tlir';c. '1 . ?Lid I'ac& c&il, Ti'ie i:a L . or I. 's, . ' v i appal \\ i&'L!)' 1 a, Le + '&r ! !ie p !! .teri rati n, bu' rate of. 6 in s:iri ef f icieiir y of fe c utiliza- Lier& were r&nt sf ic ted...

  8. An investigation of meson spectroscopy on isotropic clover lattices at the SU(3) flavor-symmetric point

    SciTech Connect (OSTI)

    Richards, David G.; Orginos, Konstantinos

    2014-06-23

    We present an investigation of the excited meson spectrum at the N_f= 3 point obtained on isotropic clover lattices with a plaquette Wilson gauge action, and a NP-improved clover fermion action, at a lattice spacing of a \\simeq 0.08 fm, and compare with corresponding calculations on an anisotropic lattice at fine temporal lattice spacing but a spatial lattice spacing of a_s \\simeq 0.125 fm. The methodology adopted follows that employed in the calculation of the spectrum on anisotropic lattices, and we test the efficacy of that approach for isotropic lattices. In particular, we explore the extent to which rotational symmetry for predominantly single-hadron states is realized. By comparison of the energy levels with that obtained using the anisotropic lattice, we obtain an indication of discretization uncertainties in the single-hadron spectrum.

  9. Preliminary results obtained with an apparatus for studying the effect of carbon dioxide on consumption of oxygen by respiring cotton roots 

    E-Print Network [OSTI]

    Hinesly, Thomas Daniel

    1956-01-01

    . ' diX'~ X'nsion. and 9ie xate of, oonsuaybion'of 'oxygen v@th the evolution, c4' . eax"%on-dioxide by biologiosX 'msp~5i'on+ . I Xnedegnste, Wainagsp poof' soil stPQotoxe ox" Xsx'gk s~. ~ nants . nf. : ~~, 'cteeoxXpoeable:ox genie xsxtex isis~ 'alan...'~', . ', , MC~1@Jtj@$48P. .WQ, : (Q2yg )' g' (Oyer'g6$' S~ O'QW~'6LBg, e x gsmteq . ovex' a}. 3, : yxxgi exx. eeIyep4Xoxx" amC. , eax, 'ben ', KMc lkheppPIon M RIXc~~Qlftiex'Bp;FBI x GQpfpBtex'q? ". ' ' jvytte5te'eAexxl~teC:, 6~@, 'Qie "4 eat''xxexxt 4aii...

  10. Labdanolic acid methyl ester (LAME) exerts anti-inflammatory effects through inhibition of TAK-1 activation

    SciTech Connect (OSTI)

    Cuadrado, Irene [Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense, Plaza Ramón y Cajal s/n, 28040 Madrid (Spain)] [Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense, Plaza Ramón y Cajal s/n, 28040 Madrid (Spain); Cidre, Florencia; Herranz, Sandra [Unidad de Inflamación y Cáncer. Área de Biología Celular y Desarrollo. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid (Spain)] [Unidad de Inflamación y Cáncer. Área de Biología Celular y Desarrollo. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid (Spain); Estevez-Braun, Ana [Instituto Universitario de Bio-Orgánica “Antonio González”. Universidad de La Laguna. Avda. Astrofísico Fco. Sánchez 2. 38206. La Laguna, Tenerife (Spain) [Instituto Universitario de Bio-Orgánica “Antonio González”. Universidad de La Laguna. Avda. Astrofísico Fco. Sánchez 2. 38206. La Laguna, Tenerife (Spain); Instituto Canario de Investigaciones del Cáncer (ICIC) (Spain); Heras, Beatriz de las, E-mail: lasheras@farm.ucm.es [Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense, Plaza Ramón y Cajal s/n, 28040 Madrid (Spain); Hortelano, Sonsoles, E-mail: shortelano@isciii.es [Unidad de Inflamación y Cáncer. Área de Biología Celular y Desarrollo. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid (Spain)] [Unidad de Inflamación y Cáncer. Área de Biología Celular y Desarrollo. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid (Spain)

    2012-01-01

    Labdane derivatives obtained from the diterpenoid labdanediol suppressed NO and PGE{sub 2} production in LPS-stimulated RAW 264.7 macrophages. However, mechanisms involved in these inhibitory effects are not elucidated. In this study, we investigated the signaling pathways involved in the anti-inflammatory effects of labdanolic acid methyl ester (LAME) in peritoneal macrophages and examined its therapeutic effect in a mouse endotoxic shock model. LAME reduced the production of NO and PGE{sub 2} in LPS-activated macrophages. This effect involved the inhibition of NOS-2 and COX-2 gene expression, acting at the transcription level. Examination of the effects of the diterpene on NF-?B signaling showed that LAME inhibits the phosphorylation of I?B? and I?B?, preventing their degradation and the nuclear translocation of the NF-?B p65 subunit. Moreover, inhibition of MAPK signaling was also observed. A further experiment revealed that LAME inhibited the phosphorylation of transforming growth factor-? (TGF-?)-activated kinase 1 (TAK1), an upstream signaling molecule required for IKK and mitogen-activated protein kinases (MAPKs) activation. Inflammatory cytokines such as IL-6, TNF-? and IP-10 were downregulated in the presence of this compound after stimulation with LPS. Additionally, LAME also improved survival in a mouse model of endotoxemia and reduced the circulatory levels of cytokines (IL-6, TNF-?). In conclusion, these results indicate that labdane diterpene LAME significantly attenuates the pro-inflammatory response induced by LPS both in vivo and in vitro. Highlights: ? LAME reduced the production of NO and PGE{sub 2} in LPS-activated macrophages. ? IL-6, TNF-? and IP-10 were also inhibited by LAME. ? Inhibition of TAK-1 activation is the mechanism involved in this process. ? LAME improved survival in a mouse model of endotoxemia. ? LAME reduced the circulatory levels of cytokines (IL-6, TNF-?).

  11. Heavy-light quarks interactions in QCD vacuum

    E-Print Network [OSTI]

    Mirzayusuf Musakhanov

    2015-03-20

    QCD vacuum instantons induce very strong interactions between light quarks, which generate large dynamical light quark mass M for initially almost massless quarks and can bound these quarks to produce almost massless pions in accordance with the spontaneous breaking of chiral symmetry (S\\chiSB). On the other hand, the QCD vacuum instantons generate heavy-light quark interactions terms, which are responsible for the effects of S\\chiSB in a heavy-light quark system. Summing the re-scattering series that lead to the total light quark propagator and making few further steps, we get the fermionized representation of low-frequencies light quark determinant in the presence of the quark sources, which is relevant for our problems. The next important step in the line of this strategy is to derive the equation and calculate the heavy quark propagator in the instanton media and in the presence of light quarks. This one provide finally the heavy and N_f light quarks interaction term. As an example, we derive heavy quark-light mesons interaction term for the N_f=2 case. If we take the average instanton size \\rho=0.35 fm, and average inter-instanton distance R=0.856 fm from our previous estimates, we obtain at LO on 1/N_c expansion dynamical light quark mass M = 570 MeV and instanton media contribution to heavy quark mass \\Delta M=148 MeV. These factors define the coupling between heavy and light quarks and, certainly, between heavy quarks and light mesons. We will apply this approach to heavy quark and heavy-light quark systems.

  12. Separation of metallic residues from the dissolution of a high-burnup BWR fuel using nitrogen trifluoride

    SciTech Connect (OSTI)

    McNamara, Bruce K.; Buck, Edgar C.; Soderquist, Chuck Z.; Smith, Frances N.; Mausolf, Edward J.; Scheele, Randall D.

    2014-02-10

    Nitrogen trifluoride (NF3) was used to fluorinate the metallic residue from the dissolution of a high burnup, boiling water reactor fuel (?70 MWd/kgU). The metallic residue included the noble metal phase (containing ruthenium, rhodium, palladium, technetium, and molybdenum), and smaller amounts of zirconium, selenium, tellurium, and silver. Exposing the noble metal phase to 10% NF3 in argon between 400 and 550?C, removed molybdenum and technetium near 400?C as their volatile fluorides, and ruthenium near 500?C as its volatile fluoride. The events were thermally and temporally distinct and the conditions specified are a recipe to separate these transition metals from each other and from the noble metal phase nonvolatile residue. Depletion of the volatile fluorides resulted in substantial exothermicity. Thermal excursion behavior was recorded under non-adiabatic, isothermal conditions that typically minimize heat release. Physical characterization of the metallic noble phase and its thermal behavior are consistent with high kinetic velocity reactions encouraged by the nanoparticulate phase or perhaps catalytic influences of the mixed platinum metals with nearly pure phase structure. Post-fluorination, only two phases were present in the residual nonvolatile fraction. These were identified as a nano-crystalline, metallic palladium cubic phase and a hexagonal rhodium trifluoride (RhF3) phase. The two phases were distinct as the sub-µm crystallites of metallic palladium were in contrast to the RhF3 phase, which grew from the parent nano-crystalline noble-metal phase during fluorination, to acicular crystals exceeding 20-µm in length.

  13. The innate and adaptive immune response induced by alveolar macrophages exposed to ambient particulate matter

    SciTech Connect (OSTI)

    Miyata, Ryohei; Eeden, Stephan F. van

    2011-12-15

    Emerging epidemiological evidence suggests that exposure to particulate matter (PM) air pollution increases the risk of cardiovascular events but the exact mechanism by which PM has adverse effects is still unclear. Alveolar macrophages (AM) play a major role in clearing and processing inhaled PM. This comprehensive review of research findings on immunological interactions between AM and PM provides potential pathophysiological pathways that interconnect PM exposure with adverse cardiovascular effects. Coarse particles (10 {mu}m or less, PM{sub 10}) induce innate immune responses via endotoxin-toll-like receptor (TLR) 4 pathway while fine (2.5 {mu}m or less, PM{sub 2.5}) and ultrafine particles (0.1 {mu}m or less, UFP) induce via reactive oxygen species generation by transition metals and/or polyaromatic hydrocarbons. The innate immune responses are characterized by activation of transcription factors [nuclear factor (NF)-{kappa}B and activator protein-1] and the downstream proinflammatory cytokine [interleukin (IL)-1{beta}, IL-6, and tumor necrosis factor-{alpha}] production. In addition to the conventional opsonin-dependent phagocytosis by AM, PM can also be endocytosed by an opsonin-independent pathway via scavenger receptors. Activation of scavenger receptors negatively regulates the TLR4-NF-{kappa}B pathway. Internalized particles are subsequently subjected to adaptive immunity involving major histocompatibility complex class II (MHC II) expression, recruitment of costimulatory molecules, and the modulation of the T helper (Th) responses. AM show atypical antigen presenting cell maturation in which phagocytic activity decreases while both MHC II and costimulatory molecules remain unaltered. PM drives AM towards a Th1 profile but secondary responses in a Th1- or Th-2 up-regulated milieu drive the response in favor of a Th2 profile.

  14. Activation of IL-2 receptor {alpha}-chain gene by individual members of the rel oncogene family in association with serum response factor

    SciTech Connect (OSTI)

    Pierce, J.W.; Jamieson, C.A.; Ross, J.L.

    1995-08-15

    Expression of the IL-2R{alpha} gene is regulated by members of the c-Rel/NF-{kappa}B family of transcription factors binding to the {kappa}B site in the promoter. Previous work has not defined the role of individual members of the c-Rel family in the activation of the IL-2R{alpha} gene. Using the COS cell system, we were able to reconstitute the regulation of the IL-2R{alpha} promoter by expressing cloned Rel family members with serum response factor (SRF). We found that c-rel alone activated the IL-2R{alpha} promoter only weakly but worked with the p50 subunit of NF-{kappa}B (NFKB1) to give a higher level of expression. We showed that c-rel heterodimerizes with p50 and the amount of this heterodimer correlated with the level of IL-2R{alpha} gene expression. Our results provide evidence that c-rel/p50 heterodimers activate gene expression in the context of a cellular promoter. We show that c-rel or p65 can cooperate with SRF in the activation of this promoter and the transactivation by c-rel with SRF was enhanced by p50. Synergistic activation required both {kappa}B an CArG sites, and binding studies show that these that these adjacent sites can be occupied simultaneously. The transactivation observed with cloned transcription factors mimics the physiologic induction of the IL-2R{alpha} gene since multiple sequence elements cooperate to give gene activation. The data support the model that c-rel/p50 or p65 can cooperate with SRF to specifically target the expression of the IL-2R{alpha} gene in activated T cells.

  15. Spent nuclear fuel reprocessing modeling

    SciTech Connect (OSTI)

    Tretyakova, S.; Shmidt, O.; Podymova, T.; Shadrin, A.; Tkachenko, V. [Bochvar Institute, 5 Rogova str., Moscow 123098 (Russian Federation); Makeyeva, I.; Tkachenko, V.; Verbitskaya, O.; Schultz, O.; Peshkichev, I. [Russian Federal Nuclear Center - VNIITF E.I. Zababakhin, p.o.box 245, Snezhinsk, 456770 (Russian Federation)

    2013-07-01

    The long-term wide development of nuclear power requires new approaches towards the realization of nuclear fuel cycle, namely, closed nuclear fuel cycle (CNFC) with respect to fission materials. Plant nuclear fuel cycle (PNFC), which is in fact the reprocessing of spent nuclear fuel unloaded from the reactor and the production of new nuclear fuel (NF) at the same place together with reactor plant, can be one variant of CNFC. Developing and projecting of PNFC is a complicated high-technology innovative process that requires modern information support. One of the components of this information support is developed by the authors. This component is the programme conducting calculations for various variants of process flow sheets for reprocessing SNF and production of NF. Central in this programme is the blocks library, where the blocks contain mathematical description of separate processes and operations. The calculating programme itself has such a structure that one can configure the complex of blocks and correlations between blocks, appropriate for any given flow sheet. For the ready sequence of operations balance calculations are made of all flows, i.e. expenses, element and substance makeup, heat emission and radiation rate are determined. The programme is open and the block library can be updated. This means that more complicated and detailed models of technological processes will be added to the library basing on the results of testing processes using real equipment, in test operating mode. The development of the model for the realization of technical-economic analysis of various variants of technologic PNFC schemes and the organization of 'operator's advisor' is expected. (authors)

  16. Effects of low dose treatment of tributyltin on the regulation of estrogen receptor functions in MCF-7 cells

    SciTech Connect (OSTI)

    Sharan, Shruti; Nikhil, Kumar; Roy, Partha, E-mail: paroyfbs@iitr.ernet.in

    2013-06-01

    Endocrine disrupting chemicals are the natural/synthetic compounds which mimic or inhibit the actions of endogenous hormones. Organotin compounds, such as tributyltin (TBT) are typical environmental contaminants and suspected endocrine-disrupting chemical. The present study evaluates the estrogenic potential of this compound in vitro in ER (+) breast adenocarcinoma, MCF-7 cell line. Our data showed that tributyltin chloride (TBTCl) had agonistic activities for estrogen receptor-? (ER-?). Its estrogenic potential was checked using cell proliferation assay, aromatase assay, transactivation assay, and protein expression analysis. Low dose treatment of TBTCl had a proliferative effect on MCF-7 cells and resulted in up-regulation of aromatase enzyme activity and enhanced estradiol production in MCF-7 cells. Immunofluorescence staining showed translocation of ER-? from cytoplasm to nucleus and increased expression of ER-?, 3?-HSD and aromatase on treatment with increasing doses of TBTCl. Further, to decipher the probable signaling pathways involved in its action, the MCF-7 cells were transfected with different pathway dependent luciferase reporter plasmids (CRE, SRE, NF-?B and AP1). A significant increase in CRE and SRE and decrease in NF-?B regulated pathway were observed (p < 0.05). Our results thus showed that the activation of SRE by TBTCl may be due to ligand dependent ER-? activation of the MAPK pathway and increased phosphorylation of ERK. In summary, the present data suggests that low dose of tributyltin genomically and non-genomically augmented estrogen dependent signaling by targeting various pathways. - Highlights: • Tributyltin chloride is agonistic to ER-? in MCF-7 cell line at low doses. • Tributyltin chloride up regulated aromatase activity and estradiol production. • Tributyltin chloride also activates MAPK pathway inducing ERK activation.

  17. The chitinase-like protein YKL-40 increases mucin5AC production in human bronchial epithelial cells

    SciTech Connect (OSTI)

    Liu, Chunyi; Li, Qi [Division of Respiratory Medicine, Second Affiliated Hospital, Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010 (China); Zhou, Xiangdong, E-mail: zxd999@263.net [Division of Respiratory Medicine, Second Affiliated Hospital, Chongqing Medical University, No. 74, Linjiang Road, Yuzhong District, Chongqing 400010 (China); Kolosov, Victor P.; Perelman, Juliy M. [Far Eastern Scientific Center of Physiology and Pathology of Respiration, Siberian Branch, Russian Academy of Medical Sciences, Blagoveshchensk (Russian Federation)

    2013-11-01

    Mucus overproduction is an important feature in patients with chronic inflammatory airway diseases. However, the regulatory mechanisms that mediate excessive mucin production remain elusive. Recently, the level of YKL-40, a chitinase-like protein, has been found to be significantly increased in chronic inflammatory airway diseases and has been shown to be associated with the severity of these diseases. In this study, we sought to explore the effect of YKL-40 on mucin5AC (MUC5AC) production in chronic inflammatory airway diseases and the potential signaling pathways involved in this process. We found that elevated YKL-40 levels increased the mRNA and protein expression of MUC5AC in a dose- and time-dependent manner, in association with the phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear factor ?B (NF-?B), reflecting their activation. These responses were significantly suppressed by the knockdown of protease-activating receptor 2 (PAR2) with specific small interfering RNA or the inhibitors of ERK and NF-?B. YKL-40-induced MUC5AC overproduction was also effectively attenuated by the inhibitor of focal adhesion kinase (FAK). Taken together, these results imply that YKL-40 can stimulate excessive MUC5AC production through PAR2- and FAK-mediated mechanisms. - Highlights: • MUC5AC is the major secreted mucin in chronic inflammatory airway diseases. • YKL-40 is a prototype of the chitinase-like protein in mammals. • YKL-40 is an active player in chronic inflammatory airway diseases. • YKL-40 can increase MUC5AC production via PAR2-mediated pathway. • FAK is another candidate to mediate YKL-40-induced MUC5AC overexpression.

  18. Protective effects of anisodamine on cigarette smoke extract-induced airway smooth muscle cell proliferation and tracheal contractility

    SciTech Connect (OSTI)

    Xu, Guang-Ni; Yang, Kai; Xu, Zu-Peng; Zhu, Liang; Hou, Li-Na; Qi, Hong; Chen, Hong-Zhuan, E-mail: hongzhuan_chen@hotmail.com; Cui, Yong-Yao, E-mail: yongyaocui@yahoo.com.cn

    2012-07-01

    Anisodamine, an antagonist of muscarinic acetylcholine receptors (mAChRs), has been used therapeutically to improve smooth muscle function, including microvascular, intestinal and airway spasms. Our previous studies have revealed that airway hyper-reactivity could be prevented by anisodamine. However, whether anisodamine prevents smoking-induced airway smooth muscle (ASM) cell proliferation remained unclear. In this study, a primary culture of rat ASM cells was used to evaluate an ASM phenotype through the ability of the cells to proliferate and express contractile proteins in response to cigarette smoke extract (CSE) and intervention of anisodamine. Our results showed that CSE resulted in an increase in cyclin D1 expression concomitant with the G0/G1-to-S phase transition, and high expression of M2 and M3. Functional studies showed that tracheal hyper-contractility accompanied contractile marker ?-SMA high-expression. These changes, which occur only after CSE stimulation, were prevented and reversed by anisodamine, and CSE-induced cyclin D1 expression was significantly inhibited by anisodamine and the specific inhibitor U0126, BAY11-7082 and LY294002. Thus, we concluded that the protective and reversal effects and mechanism of anisodamine on CSE-induced events might involve, at least partially, the ERK, Akt and NF-?B signaling pathways associated with cyclin D1 via mAChRs. Our study validated that anisodamine intervention on ASM cells may contribute to anti-remodeling properties other than bronchodilation. -- Highlights: ? CSE induces tracheal cell proliferation, hyper-contractility and ?-SMA expression. ? Anisodamine reverses CSE-induced tracheal hyper-contractility and cell proliferation. ? ERK, PI3K, and NF-?B pathways and cyclin D1 contribute to the reversal effect.

  19. New novel cleaning technique for extending mean time between mechanical cleans in a Genus tungsten CVD reactor

    SciTech Connect (OSTI)

    Lujan, R.D.; Fleming, J.G.; Baird, J.L.; Gentry, M.S. [Sandia National Labs., Albuquerque, NM (United States). Center for Microelectronics Technologies

    1994-12-31

    During the chemical vapor deposition of blanket tungsten from the reduction of tungsten hexafluoride (WF{sub 6}), metallic parts within the reaction chamber accumulate metallic tungsten, tungsten oxyfluorides, and other related tungsten species. The usual method for removal of the chamber deposits is to open the chamber and perform a labor intensive mechanical clean, which involves the use of hydrogen peroxide (H{sub 2}O{sub 2}) and deionized (DI) water, or an in-situ fluorine-base plasma clean. The authors have investigated the use of repetitive in-situ nitrogen trifluoride (NF{sub 3}) plasma cleans during the course of operating a Genuse 8721 tungsten chemical vapor deposition reactor. The Genuse reactor has been retrofitted with self-ratchetting linear slides, which allow the wafer clamps to be extended into the NF{sub 3} plasma. They have extended the mean time between failures (MTBF) due to the use of 10 minute plasma clean every 75--100 wafers. Deposition for this process is 8,000 angstroms per wafer, using 6 deposition sites. The total tungsten deposition for a 0.5 micron tungsten plug is 4 microns, per a 25 wafer lot. Instead of a total removal of the accumulated tungsten from the chamber hardware, a partial etchback of the deposition from the wafer clamps and wafer chucks was performed. With this, sources for particles and backside deposition were eliminated. They see an increase in wafer-to-wafer uniformity, lot-to-lot repeatability, and particle reduction due to the use of frequent plasma clean. Recovery time after a plasma clean is excellent and no detrimental effects from hydrogen fluoride ``poisoning`` were seen.

  20. VI-14, a novel flavonoid derivative, inhibits migration and invasion of human breast cancer cells

    SciTech Connect (OSTI)

    Li, Fanni; Li, Chenglin; Zhang, Haiwei; Lu, Zhijian [State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009 (China)] [State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009 (China); Li, Zhiyu; You, Qidong [Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009 (China)] [Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009 (China); Lu, Na [State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009 (China)] [State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009 (China); Guo, Qinglong, E-mail: anticancer_drug@yahoo.com.cn [State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009 (China)] [State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009 (China)

    2012-06-01

    It has been well characterized that flavonoids possess pronounced anticancer potentials including anti-angiogenesis, anti-metastasis, and pro-apoptosis. Herein, we report, for the first time, that VI-14, a novel flavonoid derivative, possesses anti-cancer properties. The purpose of this study is to investigate the anti-migration and anti-invasion activities of VI-14 in breast cancer cells. Our data indicate that VI-14 inhibits adhesion, migration and invasion of MDA-MB-231 and MDA-MB-435 human breast cancer cells. MDA-MB-231 cells treated with VI-14 display reduced activities and expressions of ECM degradation-associated proteins including matrix metalloproteinase 2 (MMP-2) and 9 (MMP-9) at both the protein and mRNA levels. Meanwhile, VI-14 treatment induces an up-regulated expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) and 2 (TIMP-2) in MDA-MB-231 cells. Western blotting results show that phosphorylation levels of critical components of the MAPK signaling pathway, including ERK, JNK and P38, are dramatically decreased in VI-14-treated MDA-MB-231 cells. Furthermore, treatment of VI-14 significantly decreases the nuclear levels and the binding ability of nuclear factor-kappa B (NF-?B) and activator protein-1 (AP-1). Taken together, our data suggest that VI-14 treatment suppresses migration and motility of breast cancer cells, and VI-14 may be a potential compound for cancer therapy. Highlights: ? We report for the first time that VI-14 possesses anti-cancer properties. ? VI-14 weakens the adhesion, migration and invasion of human breast cancer cells. ? VI-14 decreases the activities and expressions of MMP-2/9. ? VI-14 suppresses the phosphorylation levels of the MAPK signaling pathway. ? VI-14 decreases the nuclear levels and the binding ability of NF-?B and AP-1.

  1. Sirt2 suppresses inflammatory responses in collagen-induced arthritis

    SciTech Connect (OSTI)

    Lin, Jiangtao; Department of Orthopaedics, Yantaishan Hospital, 91 Jiefang Road, Yantai, Shandong 264001 ; Sun, Bing; Jiang, Chuanqiang; Hong, Huanyu; Zheng, Yanping

    2013-11-29

    Highlights: •Sirt2 expression decreases in collagen-induced arthritis (CIA). •Sirt2 knockout aggravates severity of arthritis in mice with CIA. •Sirt2 knockout increases levels of pro-inflammatory factors in the serum. •Sirt2 deacetylates p65 and inhibits pro-inflammatory factors expression. •Sirt2 rescue abates severity of arthritis in mice with CIA. -- Abstract: Arthritis is a common autoimmune disease that is associated with progressive disability, systemic complications and early death. However, the underling mechanisms of arthritis are still unclear. Sirtuins are a NAD{sup +}-dependent class III deacetylase family, and regulate cellular stress, inflammation, genomic stability, carcinogenesis, and energy metabolism. Among the sirtuin family members, Sirt1 and Sirt6 are critically involved in the development of arthritis. It remains unknown whether other sirtuin family members participate in arthritis. Here in this study, we demonstrate that Sirt2 inhibits collagen-induced arthritis (CIA) using in vivo and in vitro evidence. The protein and mRNA levels of Sirt2 significantly decreased in joint tissues of mice with CIA. When immunized with collagen, Sirt2-KO mice showed aggravated severity of arthritis based on clinical scores, hind paw thickness, and radiological and molecular findings. Mechanically, Sirt2 deacetylated p65 subunit of nuclear factor-kappa B (NF-?B) at lysine 310, resulting in reduced expression of NF-?B-dependent genes, including interleukin 1? (IL-1?), IL-6, monocyte chemoattractant protein 1(MCP-1), RANTES, matrix metalloproteinase 9 (MMP-9) and MMP-13. Importantly, our rescue experiment showed that Sirt2 re-expression abated the severity of arthritis in Sirt2-KO mice. Those findings strongly indicate Sirt2 as a considerably inhibitor of the development of arthritis.

  2. Code qualification of structural materials for AFCI advanced recycling reactors.

    SciTech Connect (OSTI)

    Natesan, K.; Li, M.; Majumdar, S.; Nanstad, R.K.; Sham, T.-L.

    2012-05-31

    This report summarizes the further findings from the assessments of current status and future needs in code qualification and licensing of reference structural materials and new advanced alloys for advanced recycling reactors (ARRs) in support of Advanced Fuel Cycle Initiative (AFCI). The work is a combined effort between Argonne National Laboratory (ANL) and Oak Ridge National Laboratory (ORNL) with ANL as the technical lead, as part of Advanced Structural Materials Program for AFCI Reactor Campaign. The report is the second deliverable in FY08 (M505011401) under the work package 'Advanced Materials Code Qualification'. The overall objective of the Advanced Materials Code Qualification project is to evaluate key requirements for the ASME Code qualification and the Nuclear Regulatory Commission (NRC) approval of structural materials in support of the design and licensing of the ARR. Advanced materials are a critical element in the development of sodium reactor technologies. Enhanced materials performance not only improves safety margins and provides design flexibility, but also is essential for the economics of future advanced sodium reactors. Code qualification and licensing of advanced materials are prominent needs for developing and implementing advanced sodium reactor technologies. Nuclear structural component design in the U.S. must comply with the ASME Boiler and Pressure Vessel Code Section III (Rules for Construction of Nuclear Facility Components) and the NRC grants the operational license. As the ARR will operate at higher temperatures than the current light water reactors (LWRs), the design of elevated-temperature components must comply with ASME Subsection NH (Class 1 Components in Elevated Temperature Service). However, the NRC has not approved the use of Subsection NH for reactor components, and this puts additional burdens on materials qualification of the ARR. In the past licensing review for the Clinch River Breeder Reactor Project (CRBRP) and the Power Reactor Innovative Small Module (PRISM), the NRC/Advisory Committee on Reactor Safeguards (ACRS) raised numerous safety-related issues regarding elevated-temperature structural integrity criteria. Most of these issues remained unresolved today. These critical licensing reviews provide a basis for the evaluation of underlying technical issues for future advanced sodium-cooled reactors. Major materials performance issues and high temperature design methodology issues pertinent to the ARR are addressed in the report. The report is organized as follows: the ARR reference design concepts proposed by the Argonne National Laboratory and four industrial consortia were reviewed first, followed by a summary of the major code qualification and licensing issues for the ARR structural materials. The available database is presented for the ASME Code-qualified structural alloys (e.g. 304, 316 stainless steels, 2.25Cr-1Mo, and mod.9Cr-1Mo), including physical properties, tensile properties, impact properties and fracture toughness, creep, fatigue, creep-fatigue interaction, microstructural stability during long-term thermal aging, material degradation in sodium environments and effects of neutron irradiation for both base metals and weld metals. An assessment of modified versions of Type 316 SS, i.e. Type 316LN and its Japanese version, 316FR, was conducted to provide a perspective for codification of 316LN or 316FR in Subsection NH. Current status and data availability of four new advanced alloys, i.e. NF616, NF616+TMT, NF709, and HT-UPS, are also addressed to identify the R&D needs for their code qualification for ARR applications. For both conventional and new alloys, issues related to high temperature design methodology are described to address the needs for improvements for the ARR design and licensing. Assessments have shown that there are significant data gaps for the full qualification and licensing of the ARR structural materials. Development and evaluation of structural materials require a variety of experimental facilities that have been seriously degraded

  3. Near-field/altered-zone models report

    SciTech Connect (OSTI)

    Hardin, E. L., LLNL

    1998-03-01

    The U.S. Department of Energy is studying Yucca Mountain as the possible site for the first underground repository for permanent disposal of spent fuel from commercial nuclear reactors as well as for other types high-level nuclear waste. Emplacement of high-level radioactive waste, especially commercial spent nuclear fuel (CSNF), in Yucca Mountain will release a large amount of heat into the rock above and below the repository. The heating rate will decrease with time, creating a thermal pulse. Over a period of several thousand years, the rock temperature will rise initially, then drop when the production of decay heat falls below the rate at which heat escapes from the hot zone. Besides raising the rock temperature, much of this heat will vaporize water, which will then condense in cooler regions. The condensate is likely to form a gravity-driven heat pipe above the repository, creating the possibility that water may drain back onto the waste packages (WPs) or that it may ''shed'' through the pillars between emplacement drifts. The long-term importance of these effects has been investigated through the development, testing, and application of thermohydrologic (TH) models. Other effects, such coupled chemical and mechanical processes, may also influence the movement of water above, within, and below the emplacement drifts. A recent report on thermally driven coupled processes (Hardin and Chesnut, 1997) provides a qualitative assessment of the probable significance of these processes for the Yucca Mountain Site Characterization Project (YMSCP) and is the phenomenological framework for the present report. This report describes the conceptual and numerical models that have been developed to predict the thermal, mechanical, hydrologic, and chemical responses to the cumulative heat production of the potential host rock at Yucca Mountain. As proposed, the repository horizon will be situated within the Topopah Spring tuff, in the adjacent middle nonlithophysal and lower lithophysal units. These units are made up of moderately to densely welded, devitrified, fractured tuff. The rock's chemical composition is comparable to that of typical granite, but has textural features and mineralogical characteristics of large-scale, silicic volcanism. Because the repository horizon will be approximately 300 m below the ground surface and 200 m above the water table, the repository will be partially saturated. The welded tuff matrix in the host units is highly impermeable, but water and gas flow readily through fractures. The degree of fracturing in these units is highly variable, and the hydrologic significance of fracturing is an important aspect of site investigation. This report describes the characterization and modeling of a region around the potential repository--the altered zone--a region in which the temperature will be increased significantly by waste-generated heat. Numerical simulation has shown that, depending on the boundary conditions, rock properties, and repository design features incorporated in the models, the altered zone (AZ) may extend from the water table to the ground surface. This report also describes models of the near field, the region comprising the repository emplacement drifts and the surrounding rock, which are critical to the performance of engineered components. Investigations of near-field and altered-zone (NF/AZ) processes support the design of underground repository facilities and engineered barriers and also provide constraint data for probabilistic calculations of waste-isolation performance (i.e., performance assessment). The approach to investigation, which is an iterative process involving hypothesis testing and experimentation, has relied on conceptualizing engineered barriers and on performance analysis. This report is a collection, emphasizing conceptual and numerical models, of the recent results contributed from studies of NF/AZ processes and of quantitative measures of NF/AZ performance. The selection and presentation of contributions are intended to show the iterative development of understand

  4. Real-time Molecular Study of Bystander Effects of Low dose Low LET radiation Using Living Cell Imaging and Nanoparticale Optics

    SciTech Connect (OSTI)

    Natarajan, Mohan; Xu, Nancy R; Mohan, Sumathy

    2013-06-03

    In this study two novel approaches are proposed to investigate precisely the low dose low LET radiation damage and its effect on bystander cells in real time. First, a flow shear model system, which would provide us a near in vivo situation where endothelial cells in the presence of extra cellular matrix experiencing continuous flow shear stress, will be used. Endothelial cells on matri-gel (simulated extra cellular matrix) will be subjected to physiological flow shear (that occurs in normal blood vessels). Second, a unique tool (Single nano particle/single live cell/single molecule microscopy and spectroscopy; Figure A) will be used to track the molecular trafficking by single live cell imaging. Single molecule chemical microscopy allows one to single out and study rare events that otherwise might be lost in assembled average measurement, and monitor many target single molecules simultaneously in real-time. Multi color single novel metal nanoparticle probes allow one to prepare multicolor probes (Figure B) to monitor many single components (events) simultaneously and perform multi-complex analysis in real-time. These nano-particles resist to photo bleaching and hence serve as probes for unlimited timeframe of analysis. Single live cell microscopy allows one to image many single cells simultaneously in real-time. With the combination of these unique tools, we will be able to study under near-physiological conditions the cellular and sub-cellular responses (even subtle changes at one molecule level) to low and very low doses of low LET radiation in real time (milli-second or nano-second) at sub-10 nanometer spatial resolution. This would allow us to precisely identify, at least in part, the molecular mediators that are responsible of radiation damage in the irradiated cells and the mediators that are responsible for initiating the signaling in the neighboring cells. Endothelial cells subjected to flow shear (2 dynes/cm2 or 16 dynes/cm2) and exposed to 0.1, 1 and 10 cGy on coverslips will be examined for (a) low LET radiation-induced alterations of cellular function and its physiological relevance in real time; and (b) radiation damage triggered bystander effect on the neighboring unirradiated cells. First, to determine the low LET radiation induced alteration of cellular function we will examine: (i) the real time transformation of single membrane transporters in single living cells; (ii) the pump efficiency of membrane efflux pump of live cells in real time at the molecular level; (iii) the kinetics of single-ligand receptor interaction on single live cell surface (Figure C); and (iv) alteration in chromosome replication in living cell. Second, to study the radiation triggered bystander responses, we will examine one of the key signaling pathway i.e. TNF- alpha/NF-kappa B mediated signaling. TNF-alpha specific nano particle sensors (green) will be developed to detect the releasing dynamics, transport mechanisms and ligand-receptor binding on live cell surface in real time. A second sensor (blue) will be developed to simultaneously monitor the track of NF-kB inside the cell. The proposed nano-particle optics approach would complement our DOE funded study on biochemical mechanisms of TNF-alpha- NF-kappa B-mediated bystander effect.

  5. Microscale Immune Studies Laboratory.

    SciTech Connect (OSTI)

    Poschet, Jens Fredrich; Carroll-Portillo, Amanda; Wu, Meiye; Manginell, Ronald Paul; Herr, Amy Elizabeth; Martino, Anthony A.; Perroud, Thomas D.; Branda, Catherine; Srivastava, Nimisha; Sinclair, Michael B.; Moorman, Matthew Wallace; Apblett, Christopher Alan; Sale, Kenneth L.; James, Conrad D.; Carles, Elizabeth L.; Lidke, Diane S. (University of New Mexico, Albuquerque, NM); Van Benthem, Mark Hilary; Rebeil, Roberto; Kaiser, Julie; Seaman, William (University of California, San Francisco, CA); Rempe, Susan; Brozik, Susan Marie; Jones, Howland D. T.; Gemperline, Paul (East Carolina University, Greenville, NC); Throckmorton, Daniel J.; Misra, Milind; Murton, Jaclyn K.; Carson, Bryan D.; Zhang, Zhaoduo; Plimpton, Steven James; Renzi, Ronald F.; Lane, Todd W.; Ndiaye-Dulac, Elsa; Singh, Anup K.; Haaland, David Michael; Faulon, Jean-Loup Michel; Davis, Ryan W.; Ricken, James Bryce; Branda, Steven S.; Patel, Kamlesh D.; Joo, Jaewook; Kubiak, Glenn D.; Brennan, James S.; Martin, Shawn Bryan; Brasier, Allan (University of Texas Mecial Branch, Galveston, TX)

    2009-01-01

    The overarching goal is to develop novel technologies to elucidate molecular mechanisms of the innate immune response in host cells to pathogens such as bacteria and viruses including the mechanisms used by pathogens to subvert/suppress/obfuscate the immune response to cause their harmful effects. Innate immunity is our first line of defense against a pathogenic bacteria or virus. A comprehensive 'system-level' understanding of innate immunity pathways such as toll-like receptor (TLR) pathways is the key to deciphering mechanisms of pathogenesis and can lead to improvements in early diagnosis or developing improved therapeutics. Current methods for studying signaling focus on measurements of a limited number of components in a pathway and hence, fail to provide a systems-level understanding. We have developed a systems biology approach to decipher TLR4 pathways in macrophage cell lines in response to exposure to pathogenic bacteria and their lipopolysaccharide (LPS). Our approach integrates biological reagents, a microfluidic cell handling and analysis platform, high-resolution imaging and computational modeling to provide spatially- and temporally-resolved measurement of TLR-network components. The Integrated microfluidic platform is capable of imaging single cells to obtain dynamic translocation data as well as high-throughput acquisition of quantitative protein expression and phosphorylation information of selected cell populations. The platform consists of multiple modules such as single-cell array, cell sorter, and phosphoflow chip to provide confocal imaging, cell sorting, flow cytomtery and phosphorylation assays. The single-cell array module contains fluidic constrictions designed to trap and hold single host cells. Up to 100 single cells can be trapped and monitored for hours, enabling detailed statistically-significant measurements. The module was used to analyze translocation behavior of transcription factor NF-kB in macrophages upon activation by E. coli and Y. pestis LPS. The chip revealed an oscillation pattern in translocation of NF-kB indicating the presence of a negative feedback loop involving IKK. Activation of NF-kB is preceded by phosphorylation of many kinases and to correlate the kinase activity with translocation, we performed flow cytometric assays in the PhosphoChip module. Phopshorylated forms of p38. ERK and RelA were measured in macrophage cells challenged with LPS and showed a dynamic response where phosphorylation increases with time reaching a maximum at {approx}30-60min. To allow further downstream analysis on selected cells, we also implemented an optical-trapping based sorting of cells. This has allowed us to sort macrophages infected with bacteria from uninfected cells with the goal of obtaining data only on the infected (the desired) population. The various microfluidic chip modules and the accessories required to operate them such as pumps, heaters, electronic control and optical detectors are being assembled in a bench-top, semi-automated device. The data generated is being utilized to refine existing TLR pathway model by adding kinetic rate constants and concentration information. The microfluidic platform allows high-resolution imaging as well as quantitative proteomic measurements with high sensitivity (

  6. Fresh Water Generation from Aquifer-Pressured Carbon Storage: Interim Progress Report

    SciTech Connect (OSTI)

    Aines, R D; Wolery, T J; Hao, Y; Bourcier, W L

    2009-07-22

    This project is establishing the potential for using brine pressurized by Carbon Capture and Storage (CCS) operations in saline formations as the feedstock for desalination and water treatment technologies including nanofiltration (NF) and reverse osmosis (RO). The aquifer pressure resulting from the energy required to inject the carbon dioxide provides all or part of the inlet pressure for the desalination system. Residual brine would be reinjected into the formation at net volume reduction. This process provides additional storage space (capacity) in the aquifer, reduces operational risks by relieving overpressure in the aquifer, and provides a source of low-cost fresh water to offset costs or operational water needs. Computer modeling and laboratory-scale experimentation are being used to examine mineral scaling and osmotic pressure limitations for brines typical of CCS sites. Computer modeling is being used to evaluate processes in the aquifer, including the evolution of the pressure field. This progress report deals mainly with our geochemical modeling of high-salinity brines and covers the first six months of project execution (September, 2008 to March, 2009). Costs and implementation results will be presented in the annual report. The brines typical of sequestration sites can be several times more concentrated than seawater, requiring specialized modeling codes typical of those developed for nuclear waste disposal calculations. The osmotic pressure developed as the brines are concentrated is of particular concern, as are precipitates that can cause fouling of reverse osmosis membranes and other types of membranes (e.g., NF). We have now completed the development associated with tasks (1) and (2) of the work plan. We now have a contract with Perlorica, Inc., to provide support to the cost analysis and nanofiltration evaluation. We have also conducted several preliminary analyses of the pressure effect in the reservoir in order to confirm that reservoir pressure can indeed be used to drive the reverse osmosis process. Our initial conclusions from the work to date are encouraging: (1) The concept of aquifer-pressured RO to provide fresh water associated with carbon dioxide storage appears feasible. (2) Concentrated brines such as those found in Wyoming are amenable to RO treatment. We have looked at sodium chloride brines from the Nugget Formation in Sublette County. 20-25% removal with conventional methods is realistic; higher removal appears achievable with NF. The less concentrated sulfate-rich brines from the Tensleep Formation in Sublette County would support >80% removal with conventional RO. (3) Brines from other proposed sequestration sites can now be analyzed readily. An osmotic pressure curve appropriate to these brines can be used to evaluate cost and equipment specifications. (4) We have examined a range of subsurface brine compositions that is potentially pertinent to carbon sequestration and noted the principal compositional trends pertinent to evaluating the feasibility of freshwater extraction. We have proposed a general categorization for the feasibility of the process based on total dissolved solids (TDS). (5) Withdrawing pressurized brine can have a very beneficial effect on reservoir pressure and total available storage capacity. Brine must be extracted from a deeper location in the aquifer than the point of CO{sub 2} injection to prevent CO{sub 2} from migrating to the brine extraction well.

  7. Final Report [The c-Abl signaling network in the radioadaptive response

    SciTech Connect (OSTI)

    Chi-Min, Yuan

    2014-01-28

    The radioadaptive response, or radiation hormesis, i.e. a low dose of radiation can protect cells and organisms from the effects of a subsequent higher dose, is a widely recognized phenomenon. Mechanisms underlying such radiation hormesis, however, remain largely unclear. Preliminary studies indicate an important role of c-Abl signaling in mediating the radioadaptive response. We propose to investigate how c-Abl regulates the crosstalk between p53 and NF?B in response to low doses irradiation. We found in our recent study that low dose IR induces a reciprocal p53 suppression and NF?B activation, which induces HIF-a and subsequently a metabolic reprogramming resulting in a transition from oxidative phosphorylation to glycolysis. Of importance is that this glycolytic switch is essential for the radioadaptive response. This low-dose radiationinduced HIF1? activation was in sharp contrast with the high-dose IR-induced p53 activation and HIF1? inhibition. HIF1? and p53 seem to play distinct roles in mediating the radiation dose-dependent metabolic response. The induction of HIF1?-mediated glycolysis is restricted to a low dose range of radiation, which may have important implications in assessing the level of radiation exposure and its potential health risk. Our results support a dose-dependent metabolic response to IR. When IR doses are below the threshold of causing detectable DNA damage (<0.2Gy) and thus little p53 activation, HIF1? is induced resulting in induction of glycolysis and increased radiation resistance. When the radiation dose reaches levels eliciting DNA damage, p53 is activated and diminishes the activity of HIF1? and glycolysis, leading to the induction of cell death. Our work challenges the LNT model of radiation exposure risk and provides a metabolic mechanism of radioadaptive response. The study supports a need for determining the p53 and HIF1? activity as a potential reliable biological readout of radiation exposure in humans. The exquisite sensitivity of cellular metabolism to low doses of radiation could also serve as a valuable biomarker for estimating the health effects of low-level radiation exposure.

  8. Transcriptome analysis of the human T lymphocyte cell line Jurkat and human peripheral blood mononuclear cells exposed to deoxynivalenol (DON): New mechanistic insights

    SciTech Connect (OSTI)

    Katika, Madhumohan R.; Department of Health Risk Analysis and Toxicology, Maastricht University; Netherlands Toxicogenomics Centre ; Hendriksen, Peter J.M.; Netherlands Toxicogenomics Centre ; Shao, Jia; Department of Health Risk Analysis and Toxicology, Maastricht University; Netherlands Toxicogenomics Centre ; Loveren, Henk van; National Institute for Public Health and the Environment , Bilthoven; Netherlands Toxicogenomics Centre ; Peijnenburg, Ad; Netherlands Toxicogenomics Centre

    2012-10-01

    Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid cells. For this, we exposed the human T-lymphocyte cell line Jurkat and human peripheral blood mononuclear cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat cells were exposed to 0.25 and 0.5 ?M DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-?B/TNF-? pathways, T cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T cell activation and apoptosis. Induction of T cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, cells were exposed to 2 and 4 ?M DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat cell line were also affected in the PBMCs. -- Highlights: ? The human T cell line Jurkat and human PBMCs were exposed to DON. ? Whole-genome microarray experiments were performed. ? Microarray data indicates that DON affects ribosome and RNA/protein synthesis. ? DON treatment induces ER stress, calcium mediated signaling, NFAT and NF-?B. ? Exposure to DON induces T cell activation, oxidative stress and apoptosis.

  9. Towards MQGP

    E-Print Network [OSTI]

    Mansi Dhuria; Aalok Misra

    2015-03-19

    For the Ouyang embedding we calculate the chemical potential mu_C due to a U(1) gauge field on the w.v. of N_f D7-branes wrapped around a 4-cycle in a resolved warped deformed conifold with (M)N (fractional)D3-branes of [1], and show the possible thermodynamical stability up to linear order in the embedding parameter. In the spirit of [2] we obtain the local type IIA mirror using SYZ mirror symmetry near (theta_{1,2},psi)=(,{0,2pi,,4pi}) and then oxidize the same to M theory. We take two limits of this uplift:(i)g_s,g_sN_f,g_sM^2/N,g_s^2M N_f>1 similar to [1] effected by M eps^{-3d/2}, N eps^{-19d},g_s epsn^d,d>0 and eps>1 for finite g_s,M, effected by: g_s eps, M eps^{-3d/2},N eps^{-39d},d>0, epsuplift gives a black M3-brane solution whose near-horizon geometry near theta_{1,2}=0,pi-branches, preserves 1/8 SUSY. We obtain eta/s=1/4pi for the uplift and the diffusion constant for types IIB/IIA backgrounds comes out to be ~1/T, for both limits. The D=11 SUGRA action up to O(R^4,|G_4|^2) is expected to receive dominant contributions near =0,pi due to poles. Introducing a small-angle cut-off c and using the =c,(pi-c)-local uplift the specific heat from the IR-finite part of the action (c-independent) turns out to be positive indicative of the thermodynamical stability of the uplift. An ALD-gravity-type interpretation can be given to the counter-terms for(i). Its verified that the black M3-brane entropy S r_h^3 from M-theoretic thermodynamical methods and the horizon areas of types IIB/IIA/M3-brane solutions.

  10. Phenylbutyric acid protects against carbon tetrachloride-induced hepatic fibrogenesis in mice

    SciTech Connect (OSTI)

    Wang, Jian-Qing [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China) [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China); Second Affiliated Hospital, Anhui Medical University, Hefei 230601 (China); Chen, Xi [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China)] [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China); Zhang, Cheng [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); Tao, Li [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China)] [First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China); Zhang, Zhi-Hui; Liu, Xiao-Qian [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); Xu, Yuan-Bao [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China) [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); First Affiliated Hospital, Anhui Medical University, Hefei 230022 (China); Wang, Hua [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China); Li, Jun, E-mail: lijun@ahmu.edu.cn [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China)] [School of Pharmacy, Anhui Medical University, Hefei, 230032 (China); Xu, De-Xiang, E-mail: xudex@126.com [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)] [Department of Toxicology, Anhui Medical University, Hefei, 230032 (China)

    2013-01-15

    A recent report showed that the unfolded protein response (UPR) signaling was activated in the pathogenesis of carbon tetrachloride (CCl{sub 4})-induced hepatic fibrosis. Phenylbutyric acid (PBA) is a well-known chemical chaperone that inhibits endoplasmic reticulum (ER) stress and unfolded protein response (UPR) signaling. In the present study, we investigated the effects of PBA on CCl{sub 4}-induced hepatic fibrosis in mice. All mice were intraperitoneally (i.p.) injected with CCl{sub 4} (0.15 ml/kg BW, twice per week) for 8 weeks. In CCl{sub 4} + PBA group, mice were i.p. injected with PBA (150 mg/kg, twice per day) from the beginning of CCl{sub 4} injection to the end. As expected, PBA significantly attenuated CCl{sub 4}-induced hepatic ER stress and UPR activation. Although PBA alleviated, only to a less extent, hepatic necrosis, it obviously inhibited CCl{sub 4}-induced tumor necrosis factor alpha (TNF-?) and transforming growth factor beta (TGF-?). Moreover, PBA inhibited CCl{sub 4}-induced hepatic nuclear factor kappa B (NF-?B) p65 translocation and extracellular signal-regulated kinase (ERK) and c-Jun N-terminal Kinase (JNK) phosphorylation. Interestingly, CCl{sub 4}-induced ?-smooth muscle actin (?-SMA), a marker for the initiation phase of HSC activation, was significantly attenuated in mice pretreated with PBA. Correspondingly, CCl{sub 4}-induced hepatic collagen (Col)1?1 and Col1?2, markers for the perpetuation phase of HSC activation, were inhibited in PBA-treated mice. Importantly, CCl{sub 4}-induced hepatic fibrosis, as determined using Sirius red staining, was obviously attenuated by PBA. In conclusion, PBA prevents CCl{sub 4}-induced hepatic fibrosis through inhibiting hepatic inflammatory response and HSC activation. Highlights: ? CCl{sub 4} induces hepatic ER stress, inflammation, HSC activation and hepatic fibrosis. ? PBA alleviates CCl{sub 4}-induced hepatic ER stress and UPR signaling activation. ? PBA inhibits CCl{sub 4}-induced hepatic NF-?B activation and ERK and JNK phosphorylation. ? PBA effectively protects against CCl{sub 4}-induced HSC activation and hepatic fibrosis. ? ER stress is involved in CCl{sub 4}-induced hepatic inflammation and fibrogenesis.

  11. Activation of transmembrane bile acid receptor TGR5 stimulates insulin secretion in pancreatic {beta} cells

    SciTech Connect (OSTI)

    Kumar, Divya P.; Rajagopal, Senthilkumar; Mahavadi, Sunila [Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, VA (United States)] [Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, VA (United States); Mirshahi, Faridoddin [Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA (United States)] [Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA (United States); Grider, John R. [Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, VA (United States)] [Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, VA (United States); Murthy, Karnam S., E-mail: skarnam@vcu.edu [Department of Physiology and Biophysics, Virginia Commonwealth University School of Medicine, Richmond, VA (United States); Sanyal, Arun J., E-mail: asanyal@mcvh-vcu.edu [Division of Gastroenterology, Hepatology and Nutrition, Department of Internal Medicine, Virginia Commonwealth University School of Medicine, Richmond, VA (United States)

    2012-10-26

    Highlights: Black-Right-Pointing-Pointer G protein coupled receptor TGR5 is expressed in mouse and human islets. Black-Right-Pointing-Pointer TGR5 is coupled to activation of Gs and Ca{sup 2+} release via cAMP/Epac/PLC-{epsilon} pathway. Black-Right-Pointing-Pointer Activation of TGR5 by bile salts and selective ligands causes insulin secretion. Black-Right-Pointing-Pointer TGR5 could be a potential therapeutic target to treat diabetes. -- Abstract: Bile acids act as signaling molecules and stimulate the G protein coupled receptor, TGR5, in addition to nuclear farnesoid X receptor to regulate lipid, glucose and energy metabolism. Bile acid induced activation of TGR5 in the enteroendocrine cells promotes glucagon like peptide-1 (GLP-1) release, which has insulinotropic effect in the pancreatic {beta} cells. In the present study, we have identified the expression of TGR5 in pancreatic {beta} cell line MIN6 and also in mouse and human pancreatic islets. TGR5 selective ligands, oleanolic acid (OA) and INT-777 selectively activated G{alpha}{sub s} and caused an increase in intracellular cAMP and Ca{sup 2+}. OA and INT-777 also increased phosphoinositide (PI) hydrolysis and the increase was blocked by NF449 (a selective G{alpha}{sub s} inhibitor) or (U73122) (PI hydrolysis inhibitor). OA, INT-777 and lithocholic acid increased insulin release in MIN6 and human islets and the increase was inhibited by treatment with NF449, (U73122) or BAPTA-AM (chelator of calcium), but not with myristoylated PKI (PKA inhibitor), suggesting that the release is dependent on G{sub s}/cAMP/Ca{sup 2+} pathway. 8-pCPT-2 Prime -O-Me-cAMP, a cAMP analog, which activates Epac, but not PKA also stimulated PI hydrolysis. In conclusion, our study demonstrates that the TGR5 expressed in the pancreatic {beta} cells regulates insulin secretion and highlights the importance of ongoing therapeutic strategies targeting TGR5 in the control of glucose homeostasis.

  12. Phospholipase C-{delta}{sub 1} regulates interleukin-1{beta} and tumor necrosis factor-{alpha} mRNA expression

    SciTech Connect (OSTI)

    Chung, Eric; Jakinovich, Paul; Bae, Aekyung [Department of Anesthesiology, Health Sciences Center L4 Rm 081, Stony Brook University, Stony Brook, NY 11794 (United States)] [Department of Anesthesiology, Health Sciences Center L4 Rm 081, Stony Brook University, Stony Brook, NY 11794 (United States); Rebecchi, Mario, E-mail: Mario.rebecchi@SBUmed.org [Department of Anesthesiology, Health Sciences Center L4 Rm 081, Stony Brook University, Stony Brook, NY 11794 (United States)] [Department of Anesthesiology, Health Sciences Center L4 Rm 081, Stony Brook University, Stony Brook, NY 11794 (United States)

    2012-10-01

    Phospholipase C-{delta}{sub 1} (PLC{delta}{sub 1}) is a widely expressed highly active PLC isoform, modulated by Ca{sup 2+} that appears to operate downstream from receptor signaling and has been linked to regulation of cytokine production. Here we investigated whether PLC{delta}{sub 1} modulated expression of the pro-inflammatory cytokines interleukin-1{beta} (IL-1{beta}), tumor necrosis factor-{alpha} (TNF-{alpha}) and interleukin-6 (IL-6) in rat C6 glioma cells. Expression of PLC{delta}{sub 1} was specifically suppressed by small interfering RNA (siRNA) and the effects on cytokine mRNA expression, stimulated by the Toll-like receptor (TLR) agonist, lipopolysaccharide (LPS), were examined. Real-time polymerase chain reaction (RT-PCR) results showed that PLC{delta}{sub 1} knockdown enhanced expression IL-1{beta} and tumor necrosis factor-{alpha} (TNF-{alpha}) mRNA by at least 100 fold after 4 h of LPS stimulation compared to control siRNA treatment. PLC{delta}{sub 1} knock down caused persistently high Nf{kappa}b levels at 4 h of LPS stimulation compared to control siRNA-treated cells. PLC{delta}{sub 1} knockdown was also associated with elevated nuclear levels of c-Jun after 30 min of LPS stimulation, but did not affect LPS-stimulated p38 or p42/44 MAPK phosphorylation, normally associated with TLR activation of cytokine gene expression; rather, enhanced protein kinase C (PKC) phosphorylation of cellular proteins was observed in the absence of LPS stimulation. An inhibitor of PKC, bisindolylmaleimide II (BIM), reversed phosphorylation, prevented elevation of nuclear c-Jun levels, and inhibited LPS-induced increases of IL-1{beta} and TNF-{alpha} mRNA's induced by PLC{delta}{sub 1} knockdown. Our results show that loss of PLC{delta}{sub 1} enhances PKC/c-Jun signaling and up-modulates pro-inflammatory cytokine gene transcription in concert with the TLR-stimulated p38MAPK/Nf{kappa}b pathway. Our findings are consistent with the idea that PLC{delta}{sub 1} is a suppressor of PKC activity.

  13. Case study on the utility of hepatic global gene expression profiling in the risk assessment of the carcinogen furan

    SciTech Connect (OSTI)

    Jackson, Anna Francina; Williams, Andrew; Recio, Leslie; Waters, Michael D.; Lambert, Iain B.; Yauk, Carole L.

    2014-01-01

    Furan is a chemical hepatocarcinogen in mice and rats. Its previously postulated cancer mode of action (MOA) is chronic cytotoxicity followed by sustained regenerative proliferation; however, its molecular basis is unknown. To this end, we conducted toxicogenomic analysis of B3C6F1 mouse livers following three week exposures to non-carcinogenic (0, 1, 2 mg/kg bw) or carcinogenic (4 and 8 mg/kg bw) doses of furan. We saw enrichment for pathways responsible for cytotoxicity: stress-activated protein kinase (SAPK) and death receptor (DR5 and TNF-alpha) signaling, and proliferation: extracellular signal-regulated kinases (ERKs) and TNF-alpha. We also noted the involvement of NF-kappaB and c-Jun in response to furan, which are genes that are known to be required for liver regeneration. Furan metabolism by CYP2E1 produces cis-2-butene-1,4-dial (BDA), which is required for ensuing cytotoxicity and oxidative stress. NRF2 is a master regulator of gene expression during oxidative stress and we suggest that chronic NFR2 activity and chronic inflammation may represent critical transition events between the adaptive (regeneration) and adverse (cancer) outcomes. Another objective of this study was to demonstrate the applicability of toxicogenomics data in quantitative risk assessment. We modeled benchmark doses for our transcriptional data and previously published cancer data, and observed consistency between the two. Margin of exposure values for both transcriptional and cancer endpoints were also similar. In conclusion, using furan as a case study we have demonstrated the value of toxicogenomics data in elucidating dose-dependent MOA transitions and in quantitative risk assessment. - Highlights: • Global gene expression changes in furan-exposed mouse livers were analyzed. • A molecular mode of action for furan-induced hepatocarcinogenesis is proposed. • Key pathways include NRF2, SAPK, ERK and death receptor signaling. • Important roles for TNF-alpha, c-Jun, and NF-?B in tumorigenesis are proposed. • BMD and MoE values from transcriptional and apical data are compared.

  14. Ozone and carbon dioxide effects on spider mites in white clover and peanut

    SciTech Connect (OSTI)

    Heagle, A.S.; Brandenburg, R.L.; Burns, J.C.; Miller, J.E. [North Carolina State Univ., Raleigh, NC (United States)

    1994-11-01

    Effects of O{sub 3} and/or elevated CO{sub 2} on two-spotted spider mites (Tetranychus urticae Koch) grown on an O{sub 3}-sensitive and an O{sub 3}-resistant clone of white clover (Trifolium repens L.) were measured in greenhouse and field experiments. Peanut (Arachis hypogeae L.) {open_quote}NC-9{close_quote} was used in one greenhouse study with O{sub 3}. In field studies, O{sub 3} treatments were charcoal filtered air (CF), nonfiltered air (NF), and two NF treatments with O{sub 3} added for 12 h d{sup {minus}1} at proportions of {approx} 1.25 and 1.50 times the ambient O{sub 3} concentration. In greenhouse studies, constant amounts of O{sub 3} were added to CF for 6 h d{sup {minus}1} to achieve mean concentrations ranging from 5 to 100 nL L{sup {minus}1}. For the greenhouse O{sub 3} x CO{sub 2} experiment, CO{sub 2} concentrations were ambient and approximately twice-ambient for 24 h d{sup {minus}1}. Plants were exposed to O{sub 3} and/or CO{sub 2} for {approx} 7 d before infestation with mites; daily exposures continued for 14 to 28 d to allow reproduction for at least two generations. Leaves were sampled to count eggs, larvae, nymphs, and adults. Ozone caused more chlorosis and necrosis on the O{sub 3}-sensitive clover clone (NC-S) than on the O{sub 3}-resistant clone (NC-R). Carbon dioxide enrichment increased shoot growth of both clones by {approx}33%. Statistical analyses indicated significant O{sub 3} effects in some experiments and nonsignificant O{sub 3} effects in others. A trend toward increased mite populations with increased O{sub 3} occurred, however, on NC-S in all trials. No consistent trends occurred with NC-R. With peanut, a significant linear increase in mite population occurred with increased O{sub 3}. Carbon dioxide enrichment increased the rate of population increase on both clover clones, but more so on NC-R. 47 refs., 2 figs., 7 tabs.

  15. Nitric oxide-releasing sulindac is a novel skin cancer chemopreventive agent for UVB-induced photocarcinogenesis

    SciTech Connect (OSTI)

    Chaudhary, Sandeep C.; Singh, Tripti; Kapur, Puneet; Weng, Zhiping; Arumugam, Aadithya; Elmets, Craig A. [Department of Dermatology, University of Alabama at Birmingham, 1530 3rd Avenue South, VH509, Birmingham, AL 35294-0019 (United States); Kopelovich, Levy [Division of Cancer Prevention, National Cancer Institute, 6130 Executive Blvd, Suite 2114, Bethesda, MD 20892 (United States); Athar, Mohammad, E-mail: mathar@uab.edu [Department of Dermatology, University of Alabama at Birmingham, 1530 3rd Avenue South, VH509, Birmingham, AL 35294-0019 (United States)

    2013-05-01

    Nitric oxide (NO)-releasing non-steroidal anti-inflammatory drugs (NO-NSAIDs) which have been synthesized to reduce gastro-intestinal and cardiovascular toxicities of NSAIDs, possess anti-proliferative, pro-apoptotic and anti-cancer activities. Here, we show that NO-sulindac inhibited UVB-induced skin tumorigenesis in SKH-1 hairless mice. Topical application of NO-sulindac reduced tumor incidence, number (p < 0.05) and volume (p < 0.005) as compared to UVB (alone)-irradiated vehicle-treated mice. An increase in TUNEL-positive cells in skin lesions was accompanied by the enhanced Bax:Bcl-2 ratio. The expression of pro-apoptotic Bax was increased whereas anti-apoptotic Bcl-2 reduced. However, proliferation was identified as the major target of NO-sulindac in this study. A reduced expression of PCNA and cyclin D1 associated with the dampening of cell cycle progression was observed. The mechanism of this inhibition was related to the reduction in UVB-induced Notch signaling pathway. UVB-induced inflammatory responses were diminished by NO-sulindac as observed by a remarkable reduction in the levels of phosphorylated MAP Kinases Erk1/2, p38 and JNK1/2. In this regard, NO-sulindac also inhibited NF?B by enhancing I?B? as evidenced by the reduced expression of iNOS and COX-2, the direct NF?B transcription target proteins. NO-sulindac significantly diminished the progression of benign lesions to invasive carcinomas by suppressing the tumor aggressiveness and retarding epithelial–mesenchymal transition. A marked decrease in the expression of mesenchymal markers such as Fibronectin, N-cadherin, SNAI, Slug and Twist and an increase in epithelial cell polarity marker E-cadherin were noted in NO-sulindac-treated tumors. Our data suggest that NO-sulindac is a potent inhibitor of UVB-induced skin carcinogenesis and acts by targeting proliferation-regulatory pathways. - Highlights: ? NO-sulindac is a potent chemopreventive agent for UVB-induced skin cancer. ? NO-sulindac effectively blocks proliferation. ? NO-sulindac targets Notch and RXR-PI3k/Akt pathway to achieve anti-tumor efficacy.

  16. Surface composition and taxonomic classification of a group of near-Earth and Mars-crossing asteroids

    E-Print Network [OSTI]

    Sanchez, Juan A; Reddy, Vishnu; Nathues, Andreas

    2013-01-01

    In the past, constraining the surface composition of near-Earth asteroids (NEAs) has been difficult due to the lack of high quality near-IR spectral data (0.7-2.5 microns) that contain mineralogically diagnostic absorption bands. Here we present visible (0.43-0.95 microns) and near-infrared (0.7-2.5 microns) spectra of nine NEAs and five Mars-crossing asteroids (MCs). The studied NEAs are: 4055 Magellan, 19764 (2000 NF5), 89830 (2002 CE), 138404 (2000 HA24), 143381 (2003 BC21), 159609 (2002 AQ3), 164121 (2003 YT1), 241662 (2000 KO44) and 2007 ML13. The studied MCs are: 1656 Suomi, 2577 Litva, 5407 (1992 AX), 22449 Ottijeff and 47035 (1998 WS). The observations were conducted with the NTT at La Silla, Chile, the 2.2 m telescope at Calar Alto, Spain, and the IRTF on Mauna Kea, Hawai'i. The taxonomic classification (Bus system) of asteroids showed that all observed MC asteroids belong to the S-complex, including the S, Sr and Sl classes. Seven of the NEAs belong to the S-complex, including the S, Sa, Sk and Sl c...

  17. Actinide Targets for Neutron Cross Section Measurements (C)

    SciTech Connect (OSTI)

    J. D. Baker; C. A. McGrath

    2006-04-01

    The Advanced Fuel Cycle Initiative (AFCI) and the Generation IV Reactor Initiative have demonstrated a lack of detailed neutron cross-sections for certain "minor" actinides, those other than the most common (235U, 238U, and 239Pu). For some closed-fuel-cycle reactor designs more than 50% of reactivity will, at some point, be derived from “minor” actinides that currently have poorly known (n,g) and (n,f) cross sections. A program of measurements under AFCI has begun to correct this. One of the initial hurdles has been to produce well-characterized, highly isotopically enriched, and chemically pure actinide targets on thin backings. Using a combination of resurrected techniques and new developments, we have made a series of targets including highly enriched 240Pu, and 242Pu. Thus far, we have electrodeposited these actinide targets. In the future, we plan to study reductive distillation to achieve homogeneous, adherent targets on thin metal foils and polymer backings. As we move forward, separated isotopes become scarcer, and safety concerns become greater. The chemical purification and electodeposition techniques will be described.

  18. Measurements of neutron-induced reactions in inverse kinematics and applications to nuclear astrophysics

    E-Print Network [OSTI]

    René Reifarth; Yuri A. Litvinov; Anne Endres; Kathrin Göbel; Tanja Heftrich; Jan Glorius; Alexander Koloczek; Kerstin Sonnabend; Claudia Travaglio; Mario Weigand

    2015-07-12

    Neutron capture cross sections of unstable isotopes are important for neutron-induced nucleosynthesis as well as for technological applications. A combination of a radioactive beam facility, an ion storage ring and a high flux reactor would allow a direct measurement of neutron induced reactions over a wide energy range on isotopes with half lives down to minutes. The idea is to measure neutron-induced reactions on radioactive ions in inverse kinematics. This means, the radioactive ions will pass through a neutron target. In order to efficiently use the rare nuclides as well as to enhance the luminosity, the exotic nuclides can be stored in an ion storage ring. The neutron target can be the core of a research reactor, where one of the central fuel elements is replaced by the evacuated beam pipe of the storage ring. Using particle detectors and Schottky spectroscopy, most of the important neutron-induced reactions, such as (n,$\\gamma$), (n,p), (n,$\\alpha$), (n,2n), or (n,f), could be investigated.

  19. The Condensate from Torus Knots

    E-Print Network [OSTI]

    A. Gorsky; A. Milekhin; N. Sopenko

    2015-06-22

    We discuss recently formulated instanton-torus knot duality in $\\Omega$-deformed 5D SQED on $\\mathbb{R}^4 \\times S^1$ focusing at the microscopic aspects of the condensate formation in the instanton ensemble. Using the chain of dualities and geometric transitions we embed the SQED with a surface defect into the $SU(2)$ SQCD with $N_f=4$ and identify the numbers $(n,m)$ of the torus $T_{n,m}$ knot as instanton charge and electric charge. The HOMFLY torus knot invariants in the fundamental representation provide entropic factor in the condensate of the massless flavor counting the degeneracy of the instanton--W-boson web with instanton and electric numbers $(n,m)$ but different spin and flavor content. Using the inverse geometrical transition we explain how our approach is related to the evaluation of the HOMFLY invariants in terms of Wilson loop in 3d CS theory. The reduction to 4D theory is briefly considered and some analogy with baryon vertex is conjectured.

  20. Topological charge using cooling and the gradient flow

    E-Print Network [OSTI]

    Constantia Alexandrou; Andreas Athenodorou; Karl Jansen

    2015-09-14

    The equivalence of cooling to the gradient flow when the cooling step $n_c$ and the continuous flow step of gradient flow $\\tau$ are matched is generalized to gauge actions that include rectangular terms. By expanding the link variables up to subleading terms in perturbation theory, we relate $n_c$ and $\\tau$ and show that the results for the topological charge become equivalent when rescaling $\\tau \\simeq n_c/({3-15 c_1})$ where $c_1$ is the Symanzik coefficient multiplying the rectangular term. We, subsequently, apply cooling and the gradient flow using the Wilson, the Symanzik tree-level improved and the Iwasaki gauge actions to configurations produced with $N_f=2+1+1$ twisted mass fermions. We compute the topological charge, its distribution and the correlators between cooling and gradient flow at three values of the lattice spacing demonstrating that the perturbative rescaling $\\tau \\simeq n_c/({3-15 c_1})$ leads to equivalent results.

  1. Gradient flow and scale setting on MILC HISQ ensembles

    E-Print Network [OSTI]

    MILC Collaboration; A. Bazavov; C. Bernard; N. Brown; C. DeTar; J. Foley; Steven Gottlieb; U. M. Heller; J. Komijani; J. Laiho; L. Levkova; R. L. Sugar; D. Toussaint; R. S. Van de Water

    2015-03-24

    We report on a scale determination with gradient-flow techniques on the $N_f=2+1+1$ HISQ ensembles generated by the MILC collaboration. The ensembles include four lattice spacings, ranging from approximately 0.15 to 0.06 fm, and both physical and unphysical values of the quark masses. The scales $\\sqrt{t_0}/a$ and $w_0/a$ and their tree-level improvements, $\\sqrt{t_{0,{\\rm imp}}}$ and $w_{0,{\\rm imp}}$, are computed on each ensemble using Symanzik flow and the cloverleaf definition of the energy density $E$. Using a combination of continuum chiral perturbation theory and a Taylor-series ansatz for the lattice-spacing and strong-coupling dependence, the results are simultaneously extrapolated to the continuum and interpolated to physical quark masses. We determine the scales $\\sqrt{t_0} = 0.1416({}_{-5}^{+8})$ fm and $w_0 = 0.1717({}_{-11}^{+12})$ fm, where the errors are sums, in quadrature, of statistical and all systematic errors. The precision of $w_0$ and $\\sqrt{t_0}$ is comparable to or more precise than the best previous estimates, respectively. We also find the continuum mass-dependence of $w_0$ that will be useful for estimating the scales of other ensembles. We also estimate the integrated autocorrelation length of $\\langle E(t) \\rangle$. For long flow times, the autocorrelation length of $\\langle E \\rangle$ appears to be comparable to or smaller than that of the topological charge.

  2. Topological charge using cooling and the gradient flow

    E-Print Network [OSTI]

    Alexandrou, Constantia; Jansen, Karl

    2015-01-01

    The equivalence of cooling to the gradient flow when the cooling step $n_c$ and the continuous flow step of gradient flow $\\tau$ are matched is generalized to gauge actions that include rectangular terms. By expanding the link variables up to subleading terms in perturbation theory, we relate $n_c$ and $\\tau$ and show that the results for the topological charge become equivalent when rescaling $\\tau \\simeq n_c/({3-15 c_1})$ where $c_1$ is the Symanzik coefficient multiplying the rectangular term. We, subsequently, apply cooling and the gradient flow using the Wilson, the Symanzik tree-level improved and the Iwasaki gauge actions to configurations produced with $N_f=2+1+1$ twisted mass fermions. We compute the topological charge, its distribution and the correlators between cooling and gradient flow at three values of the lattice spacing demonstrating that the perturbative rescaling $\\tau \\simeq n_c/({3-15 c_1})$ leads to equivalent results.

  3. A computer program to estimate the combined effect of refraction and diffraction of water waves 

    E-Print Network [OSTI]

    Worthington, Henry Wilson

    1970-01-01

    ), Tl luu) V(100), H(100) INTc?FR IS!, 1 JT ~ NP, IR COMMOr) PI REAL i. h)JG READ DAcx Achro Cuir'J IT IONS READ(S, IT) M, N, NQP, SP coxrIAi (3(5, FI0. 3) READ(5rlo) (lo(lrJ) l=lr ~ I)rJ=I NJ IQ FOiR HAT (F10. 3) 44 ' EAD (5, 30) ILFH(2 I TH...&BCR OF I'EQTI CAL GRID LINES ~, 2X 15, //3X, '6 ZUHdER OF Pki't1LrHS ' ?13Xr)5r//?3Xr ?GRID INTcRY/(L? )9X, F5. 1 )Xr ~ FT' 3 ~ ////?57K?'0 E P T H 5 0 U 8 D I N 0 5'I (?RITE (6 29) ((D(jr J) l=l H) ~ J=l Nf 29 FORHAT( IH //? 3K, 25F5. 2) 'HQI TE (6...

  4. Structure, depositional environment, and pressure characteristics of the Vicksburg formation: Javelina and East McCook fields, Hidalgo County, Texas 

    E-Print Network [OSTI]

    Hastings, John Olcott

    1984-01-01

    seM L La& Z-L?q LeM ueqasa[M aq& uaqM ' L!0 L laqS gno-w~eg e uo wna Lougad Esca Lg Eq pa~anoos ip see p La ib oloogoW Wse3 '0/6 [ 'qsn6n[f us 'adage [ sueaE uaag~nog (2 a LqeJ. ) Eep/33W 00[K Jog OLL' pues ?X?5unqsoloin ua~o [ aqua ?(w 6[OS o...62) 1k OSL6 wouy a6ueu uo[qM sqgdap ~e (?2? 0+ ?6? ) sauoqspues 6unqsqo lh ua~ol u i sunooo 'uaAamoq ' uo igonpoud (w 2LSV) 4k 000'9 l "a" o o'4 (w 068 l) 1k 0029 wo"4 q&dap a5ueu eave qoogoW gse3/eu[[anep aug ui sauogspues 6unqsqv[n 'a1esuapuo...

  5. Neutrino factory

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bogomilov, M.; Matev, R.; Tsenov, R.; Dracos, M.; Bonesini, M.; Palladino, V.; Tortora, L.; Mori, Y.; Planche, T.; Lagrange, J. B.; et al

    2014-12-08

    The properties of the neutrino provide a unique window on physics beyond that described by the standard model. The study of subleading effects in neutrino oscillations, and the race to discover CP-invariance violation in the lepton sector, has begun with the recent discovery that theta(13) > 0. The measured value of theta(13) is large, emphasizing the need for a facility at which the systematic uncertainties can be reduced to the percent level. The neutrino factory, in which intense neutrino beams are produced from the decay of muons, has been shown to outperform all realistic alternatives and to be capable ofmore »making measurements of the requisite precision. Its unique discovery potential arises from the fact that only at the neutrino factory is it practical to produce high-energy electron (anti) neutrino beams of the required intensity. This paper presents the conceptual design of the neutrino factory accelerator facility developed by the European Commission Framework Programme 7 EURO nu. Design Study consortium. EURO nu coordinated the European contributions to the International Design Study for the Neutrino Factory (the IDS-NF) collaboration. The EURO nu baseline accelerator facility will provide 10(21) muon decays per year from 12.6 GeV stored muon beams serving a single neutrino detector situated at a source-detector distance of between 1 500 km and 2 500 km. A suite of near detectors will allow definitive neutrino-scattering experiments to be performed.« less

  6. A new 5d description of 6d D-type minimal conformal matter

    E-Print Network [OSTI]

    Hirotaka Hayashi; Sung-Soo Kim; Kimyeong Lee; Masato Taki; Futoshi Yagi

    2015-06-18

    We propose a new 5d description of the circle-compactified 6d $(D_{N+4}, D_{N+4})$ minimal conformal matter theory which can be approached by the 6d $\\mathcal{N}=(1,0)$ $Sp(N)$ gauge theory with $N_f=2N+8$ flavors and one tensor multiplet. Compactifying the brane set-up for the 6d theory, we arrive at a 5-brane Tao diagram for 5d $\\mathcal{N}=1$ $SU(N+2)$ theory of the vanishing Chern-Simons level with $2N+8$ flavors. We conjecture that the 6d theory is recovered as the UV fixed point of this 5d theory. We show that the global symmetry of this 5d theory is $SO(4N+16)$ identical to that of the 6d theory by analyzing the 7-brane monodromy. By using the Tao diagram, we also find the instanton fugacity is exactly given by the circle radius. By decoupling flavors in this 5d theory, one can obtain all the 5d $SU(N+2)$ gauge theories of various Chern-Simons levels and corresponding enhanced global symmetries at the 5d UV fixed point.

  7. Stability of precipitate phases in Fe-rich Fe-Cr-Ni-Mo alloys

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yang, Ying; Tan, Lizhen; Busby, Jeremy T

    2015-01-01

    Understanding the stability of precipitate phases in the Fe-rich Fe-Cr-Ni-Mo alloys is critical to the alloy design and application of Mo-containing Austenitic steels. Coupled with thermodynamic modeling, stability of the and phases in two Fe-Cr-Ni-Mo alloys were investigated at 1000, 850 and 700 C for different annealing time. The morphologies, compositions and crystal structures of the matrix and precipitate phases were carefully examined by Scanning Electron Microscopy, Electron Probe Microanalysis, X-ray diffraction and Transmission Electron Microscopy. Two key findings resulted from this work. One is that the phase is stable at high temperature and transformed into the phase at lowmore »temperature. The other is that both the and phases have large solubilites of Cr, Mo and Ni, among which the Mo solubility has a major role on the relative stability of the precipitate phases. The developed thermodynamic models were then applied to evaluating the Mo effect on the stability of precipitate phases in AISI 316 and NF709 alloys.« less

  8. Monte Carlo Study of Strongly-Interacting Degenerate Fermions: a Model for Voltage-Biased Bilayer Graphene

    E-Print Network [OSTI]

    Wes Armour; Simon Hands; Costas Strouthos

    2013-02-07

    We formulate a model of N_f=4 flavors of relativistic fermion in 2+1d in the presence of a chemical potential mu coupled to two flavor doublets with opposite sign, akin to isopsin chemical potential in QCD. This is argued to be an effective theory for low energy electronic excitations in bilayer graphene, in which an applied voltage between the layers ensures equal populations of particles on one layer and holes on the other. The model is then reformulated on a spacetime lattice using staggered fermions, and in the absence of a sign problem, simulated using an orthodox hybrid Monte Carlo algorithm. With the coupling strength chosen to be close to a quantum critical point believed to exist for N_f

  9. The Deuteron and Exotic Two-Body Bound States from Lattice QCD

    E-Print Network [OSTI]

    S. R. Beane; E. Chang; W. Detmold; H. W. Lin; T. C. Luu; K. Orginos; A. Parreno; M. J. Savage; A. Torok; A. Walker-Loud

    2011-09-13

    Results of a high-statistics, multi-volume Lattice QCD exploration of the deuteron, the di-neutron, the H-dibaryon, and the Xi-Xi- system at a pion mass of m ~ 390 MeV are presented. Calculations were performed with an anisotropic n_f = 2+1 Clover discretization in four lattice volumes of spatial extent L ~ 2.0, 2.5, 3.0 and 4.0 fm, with a lattice spacing of b_s ~ 0.123 fm in the spatial-direction, and b_t ~ b_s/3.5 in the time-direction. The Xi-Xi- is found to be bound by B_{Xi-Xi-} = 14.0(1.4)(6.7) MeV, consistent with expectations based upon phenomenological models and low-energy effective field theories constrained by nucleon-nucleon and hyperon-nucleon scattering data at the physical light-quark masses. We find weak evidence that both the deuteron and the di-neutron are bound at this pion mass, with binding energies of B_d = 11(05)(12) MeV and B_{nn} = 7.1(5.2)(7.3) MeV, respectively. With an increased number of measurements and a refined analysis, the binding energy of the H-dibaryon is B_H = 13.2(1.8)(4.0) MeV at this pion mass, updating our previous result.

  10. $X(3873$ and $Y(4140)$ using diquark-antidiquark operators with lattice QCD

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Padmanath, M.; Lang, C.? B.; Prelovsek Komelj, Sasa

    2015-08-01

    We perform a lattice study of charmonium-like mesons with $J^{PC}=1^{++}$ and three quark contents $\\bar cc \\bar du$, $\\bar cc(\\bar uu+\\bar dd)$ and $\\bar cc \\bar ss$, where the later two can mix with $\\bar cc$. This simulation with $N_f=2$ and $m_\\pi=266$ MeV aims at the possible signatures of four-quark exotic states. We utilize a large basis of $\\bar cc$, two-meson and diquark-antidiquark interpolating fields, with diquarks in both anti-triplet and sextet color representations. A lattice candidate for X(3872) with I=0 is observed very close to the experimental state only if both $\\bar cc$ and $D\\bar D^*$ interpolators are included;more »the candidate is not found if diquark-antidiquark and $D\\bar D^*$ are used in the absence of $\\bar cc$. No candidate for neutral or charged X(3872), or any other exotic candidates are found in the I=1 channel. We also do not find signatures of exotic $\\bar cc\\bar ss$ candidates below 4.3 GeV, such as Y(4140). Possible physics and methodology related reasons for that are discussed. Along the way, we present the diquark-antidiquark operators as linear combinations of the two-meson operators via the Fierz transformations.« less

  11. Actinide Targets for Neutron Cross Section Measurements

    SciTech Connect (OSTI)

    John D. Baker; Christopher A. McGrath

    2006-10-01

    The Advanced Fuel Cycle Initiative (AFCI) and the Generation IV Reactor Initiative have demonstrated a lack of detailed neutron cross-sections for certain "minor" actinides, those other than the most common (235U, 238U, and 239Pu). For some closed-fuel-cycle reactor designs more than 50% of reactivity will, at some point, be derived from "minor" actinides that currently have poorly known or in some cases not measured (n,?) and (n,f) cross sections. A program of measurements under AFCI has begun to correct this. One of the initial hurdles has been to produce well-characterized, highly isotopically enriched, and chemically pure actinide targets on thin backings. Using a combination of resurrected techniques and new developments, we have made a series of targets including highly enriched 239Pu, 240Pu, and 242Pu. Thus far, we have electrodeposited these actinide targets. In the future, we plan to study reductive distillation to achieve homogeneous, adherent targets on thin metal foils and polymer backings. As we move forward, separated isotopes become scarcer, and safety concerns become greater. The chemical purification and electodeposition techniques will be described.

  12. Two Nucleon Systems at $m_\\pi\\sim 450~{\\rm MeV}$ from Lattice QCD

    E-Print Network [OSTI]

    Orginos, Kostas; Savage, Martin J; Beane, Silas R; Chang, Emmanuel; Detmold, William

    2015-01-01

    Nucleon-nucleon systems are studied with lattice quantum chromodynamics at a pion mass of $m_\\pi\\sim 450~{\\rm MeV}$ in three spatial volumes using $n_f=2+1$ flavors of light quarks. At the quark masses employed in this work, the deuteron binding energy is calculated to be $B_d = 14.4^{+3.2}_{-2.6} ~{\\rm MeV}$, while the dineutron is bound by $B_{nn} = 12.5^{+3.0}_{-5.0}~{\\rm MeV}$. Over the range of energies that are studied, the S-wave scattering phase shifts calculated in the 1S0 and 3S1-3D1 channels are found to be similar to those in nature, and indicate repulsive short-range components of the interactions, consistent with phenomenological nucleon-nucleon interactions. In both channels, the phase shifts are determined at three energies that lie within the radius of convergence of the effective range expansion, allowing for constraints to be placed on the inverse scattering lengths and effective ranges. The extracted phase shifts allow for matching to nuclear effective field theories, from which low energy...

  13. NFkappaB Selectivity of Estrogen Receptor Ligands Revealed By Comparative Crystallographic Analyses

    SciTech Connect (OSTI)

    Nettles, K.W.; Bruning, J.B.; Gil, G.; Nowak, J.; Sharma, S.K.; Hahm, J.B.; Kulp, K.; Hochberg, R.B.; Zhou, H.; Katzenellenbogen, J.A.; Katzenllenbogen, B.S.; Kim, Y.; Joachmiak, A.; Greene, G.L.

    2009-05-22

    Our understanding of how steroid hormones regulate physiological functions has been significantly advanced by structural biology approaches. However, progress has been hampered by misfolding of the ligand binding domains in heterologous expression systems and by conformational flexibility that interferes with crystallization. Here, we show that protein folding problems that are common to steroid hormone receptors are circumvented by mutations that stabilize well-characterized conformations of the receptor. We use this approach to present the structure of an apo steroid receptor that reveals a ligand-accessible channel allowing soaking of preformed crystals. Furthermore, crystallization of different pharmacological classes of compounds allowed us to define the structural basis of NF{kappa}B-selective signaling through the estrogen receptor, thus revealing a unique conformation of the receptor that allows selective suppression of inflammatory gene expression. The ability to crystallize many receptor-ligand complexes with distinct pharmacophores allows one to define structural features of signaling specificity that would not be apparent in a single structure.

  14. Inhibition of glycogen synthase kinase-3 enhances the differentiation and reduces the proliferation of adult human olfactory epithelium neural precursors

    SciTech Connect (OSTI)

    Manceur, Aziza P.; Donnelly Centre, University of Toronto, Toronto, Ontario ; Tseng, Michael; Department of Psychiatry, University of Toronto, Toronto, ON; Institute of Medical Science, University of Toronto, Toronto, ON ; Holowacz, Tamara; Witterick, Ian; Department of Otolaryngology, Head and Neck Surgery, University of Toronto, ON ; Weksberg, Rosanna; The Hospital for Sick Children, Research Institute, Program in Genetics and Genomic Biology, Toronto, Ontario Canada ; McCurdy, Richard D.; Warsh, Jerry J.; Department of Psychiatry, University of Toronto, Toronto, ON; Institute of Medical Science, University of Toronto, Toronto, ON ; Audet, Julie; Donnelly Centre, University of Toronto, Toronto, Ontario

    2011-09-10

    The olfactory epithelium (OE) contains neural precursor cells which can be easily harvested from a minimally invasive nasal biopsy, making them a valuable cell source to study human neural cell lineages in health and disease. Glycogen synthase kinase-3 (GSK-3) has been implicated in the etiology and treatment of neuropsychiatric disorders and also in the regulation of murine neural precursor cell fate in vitro and in vivo. In this study, we examined the impact of decreased GSK-3 activity on the fate of adult human OE neural precursors in vitro. GSK-3 inhibition was achieved using ATP-competitive (6-bromoindirubin-3'-oxime and CHIR99021) or substrate-competitive (TAT-eIF2B) inhibitors to eliminate potential confounding effects on cell fate due to off-target kinase inhibition. GSK-3 inhibitors decreased the number of neural precursor cells in OE cell cultures through a reduction in proliferation. Decreased proliferation was not associated with a reduction in cell survival but was accompanied by a reduction in nestin expression and a substantial increase in the expression of the neuronal differentiation markers MAP1B and neurofilament (NF-M) after 10 days in culture. Taken together, these results suggest that GSK-3 inhibition promotes the early stages of neuronal differentiation in cultures of adult human neural precursors and provide insights into the mechanisms by which alterations in GSK-3 signaling affect adult human neurogenesis, a cellular process strongly suspected to play a role in the etiology of neuropsychiatric disorders.

  15. Metallothionein-III protects against 6-hydroxydopamine-induced oxidative stress by increasing expression of heme oxygenase-1 in a PI3K and ERK/Nrf2-dependent manner

    SciTech Connect (OSTI)

    Hwang, Yong Pil; Kim, Hyung Gyun; Han, Eun Hee [BK21 Project Team, Department of Pharmacy, College of Pharmacy, Research Center for Proteineous Materials, Chosun University, Gwangju (Korea, Republic of); Jeong, Hye Gwang [BK21 Project Team, Department of Pharmacy, College of Pharmacy, Research Center for Proteineous Materials, Chosun University, Gwangju (Korea, Republic of)], E-mail: hgjeong@chosun.ac.kr

    2008-09-15

    The zinc-binding protein metallothionein-III (MT-III) is associated with resistance to neuronal injury. However, the underlying mechanism for its effects is unclear. In this study, we demonstrate that MT-III prevents the accumulation of reactive oxygen species (ROS) in dopaminergic SH-SY5Y cells challenged with the Parkinson's disease-related neurotoxin 6-hydroxydopamine (6-OHDA) by a mechanism that involves phosphatidylinositol 3-kinase (PI3K) and ERK kinase/NF-E2-related factor 2 (Nrf2) dependent induction of the stress response protein heme oxygenase-1 (HO-1). Pretreatment of SH-SY5Y cells with MT-III significantly reduced 6-OHDA-induced generation of ROS, caspase-3 activation, and subsequent cell death. Also, MT-III up-regulates HO-1 expression and this expression confers neuroprotection against oxidative injury induced by 6-OHDA. Moreover, MT-III induces Nrf2 nuclear translocation, which is upstream of MT-III-induced HO-1 expression, and PI3K and ERK1/2 activation, a pathway that is involved in induced Nrf2 nuclear translocation, HO-1 expression and neuroprotection. Taken together, these results suggest that the PI3K and ERK/Nrf2 signaling pathway controls the intracellular levels of ROS by regulating the expression of the antioxidant enzyme HO-1.

  16. Concanavalin A: A potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics

    SciTech Connect (OSTI)

    Li, Wen-wen; Yu, Jia-ying; Xu, Huai-long [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)] [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China); Bao, Jin-ku, E-mail: jinkubao@yahoo.com [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)] [School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610064 (China)

    2011-10-22

    Highlights: {yields} ConA induces cancer cell death targeting apoptosis and autophagy. {yields} ConA inhibits cancer cell angiogenesis. {yields} ConA is utilized in pre-clinical and clinical trials. -- Abstract: Concanavalin A (ConA), a Ca{sup 2+}/Mn{sup 2+}-dependent and mannose/glucose-binding legume lectin, has drawn a rising attention for its remarkable anti-proliferative and anti-tumor activities to a variety of cancer cells. ConA induces programmed cell death via mitochondria-mediated, P73-Foxo1a-Bim apoptosis and BNIP3-mediated mitochondrial autophagy. Through IKK-NF-{kappa}B-COX-2, SHP-2-MEK-1-ERK, and SHP-2-Ras-ERK anti-angiogenic pathways, ConA would inhibit cancer cell survival. In addition, ConA stimulates cell immunity and generates an immune memory, resisting to the same genotypic tumor. These biological findings shed light on new perspectives of ConA as a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis in pre-clinical or clinical trials for cancer therapeutics.

  17. The static-light baryon spectrum from twisted mass lattice QCD

    E-Print Network [OSTI]

    Marc Wagner; Christian Wiese

    2011-04-26

    We compute the static-light baryon spectrum by means of Wilson twisted mass lattice QCD using N_f = 2 flavors of sea quarks. As light u/d valence quarks we consider quarks, which have the same mass as the sea quarks with corresponding pion masses in the range 340 MeV < m_PS < 525 MeV, as well as partially quenched s quarks, which have a mass around the physical value. We consider all possible combinations of two light valence quarks, i.e. Lambda, Sigma, Xi and Omega baryons corresponding to isospin I = 0, 1/2, 1 and strangeness S = 0, -1, -2 as well as angular momentum of the light degrees of freedom j = 0, 1 and parity P = +, -. We extrapolate in the light u/d and in the heavy b quark mass to the physical point and compare with available experimental results. Besides experimentally known positive parity states we are also able to predict a number of negative parity states, which have neither been measured in experiments nor previously been computed by lattice methods.

  18. A Comparison of the Stringency of the 2001 IECC Versus the 2009 IECC and 2009 IRC 

    E-Print Network [OSTI]

    Liu, Zi; Mukhopadhyay, Jaya; Haberl, Jeff; Yazdani, Bahman; Culp, Charles

    2010-01-01

    R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 6 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 0 R - 6 A t t i c - I nf i l t r atio n N ote B 1.5 A C H D oo r s - Lo catio n and ar ea N ote B D oo r s - U Fa ct or S ec. 40 2... al m ass N ote B H ea t i ng an d cool i ng sy st em - S i z e N ote B H ea t i ng an d cool i ng sy st em - E f f i ci en cy T ab l e 503.2 S erv i ce w ater he atin g S ec. 40 2.1. 3.7 g al / da y =30*a+ ( 10 *b) T emp. : 12 0 F S erv i...

  19. Neutrino factory

    SciTech Connect (OSTI)

    Bogomilov, M.; Matev, R.; Tsenov, R.; Dracos, M.; Bonesini, M.; Palladino, V.; Tortora, L.; Mori, Y.; Planche, T.; Lagrange, J. B.; Kuno, Y.; Benedetto, E.; Efthymiopoulos, I.; Garoby, R.; Gilardoini, S.; Martini, M.; Wildner, E.; Prior, G.; Blondel, A.; Karadzhow, Y.; Ellis, M.; Kyberd, P.; Bayes, R.; Laing, A.; Soler, F. J. P.; Alekou, A.; Apollonio, M.; Aslaninejad, M.; Bontoiu, C.; Jenner, L. J.; Kurup, A.; Long, K.; Pasternak, J.; Zarrebini, A.; Poslimski, J.; Blackmore, V.; Cobb, J.; Tunnell, C.; Andreopoulos, C.; Bennett, J. R.J.; Brooks, S.; Caretta, O.; Davenne, T.; Densham, C.; Edgecock, T. R.; Fitton, M.; Kelliher, D.; Loveridge, P.; McFarland, A.; Machida, S.; Prior, C.; Rees, G.; Rogers, C.; Rooney, M.; Thomason, J.; Wilcox, D.; Booth, C.; Skoro, G.; Back, J. J.; Harrison, P.; Berg, J. S.; Fernow, R.; Gallardo, J. C.; Gupta, R.; Kirk, H.; Simos, N.; Stratakis, D.; Souchlas, N.; Witte, H.; Bross, A.; Geer, S.; Johnstone, C.; Makhov, N.; Neuffer, D.; Popovic, M.; Strait, J.; Striganov, S.; Morfín, J. G.; Wands, R.; Snopok, P.; Bagacz, S. A.; Morozov, V.; Roblin, Y.; Cline, D.; Ding, X.; Bromberg, C.; Hart, T.; Abrams, R. J.; Ankenbrandt, C. M.; Beard, K. B.; Cummings, M. A.C.; Flanagan, G.; Johnson, R. P.; Roberts, T. J.; Yoshikawa, C. Y.; Graves, V. B.; McDonald, K. T.; Coney, L.; Hanson, G.

    2014-12-08

    The properties of the neutrino provide a unique window on physics beyond that described by the standard model. The study of subleading effects in neutrino oscillations, and the race to discover CP-invariance violation in the lepton sector, has begun with the recent discovery that theta(13) > 0. The measured value of theta(13) is large, emphasizing the need for a facility at which the systematic uncertainties can be reduced to the percent level. The neutrino factory, in which intense neutrino beams are produced from the decay of muons, has been shown to outperform all realistic alternatives and to be capable of making measurements of the requisite precision. Its unique discovery potential arises from the fact that only at the neutrino factory is it practical to produce high-energy electron (anti) neutrino beams of the required intensity. This paper presents the conceptual design of the neutrino factory accelerator facility developed by the European Commission Framework Programme 7 EURO nu. Design Study consortium. EURO nu coordinated the European contributions to the International Design Study for the Neutrino Factory (the IDS-NF) collaboration. The EURO nu baseline accelerator facility will provide 10(21) muon decays per year from 12.6 GeV stored muon beams serving a single neutrino detector situated at a source-detector distance of between 1 500 km and 2 500 km. A suite of near detectors will allow definitive neutrino-scattering experiments to be performed.

  20. Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A

    SciTech Connect (OSTI)

    Van Hoof, C.; Cayla, X.; Merlevede, W.; Goris, J. [Katholieke Universiteit Leuven, Parish (France)] [and others] [Katholieke Universiteit Leuven, Parish (France); and others

    1995-07-20

    The PTPA gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. PTPA, cloned from human genomic libraries, is encoded by one single-copy gene, composed of 10 exons and 9 introns with a total length of about 60 kb. The transcription start site was determined, and the 5{prime} flanking sequence was analyzed for its potential as a promotor. This region lacks a TATA sequence in the appropriate position relative to the transcription start, is very GC-rich, and contains upstream of the transcription start four Sp1 sites, a feature common to many TATA-less promotors. Based on the homology with DNA binding consensus sequences of transcription factors, we identified in this promotor region several putative DNA binding sites for transcription factors, such as NF-{kappa}B, Myb, Ets-1, Myc, and ATF. Transfection experiments with a construct containing the PTPA promotor region inserted 5{prime} of a luciferase reporter gene revealed that the 5{prime} flanking sequence of the PTPA gene indeed displayed promotor activity that seems to be cell-line dependent. By fluorescence in situ hybridization and G-banding, the PTPA gene was localized to the 9q34 region. The PTPA gene is positioned centromeric of c-abl in a region embracing several genes implicated in oncogenesis. 28 refs., 8 figs., 1 tab.

  1. Tungsten coating for improved wear resistance and reliability of microelectromechanical devices

    DOE Patents [OSTI]

    Fleming, James G. (Albuquerque, NM); Mani, Seethambal S. (Albuquerque, NM); Sniegowski, Jeffry J. (Edgewood, NM); Blewer, Robert S. (Albuquerque, NM)

    2001-01-01

    A process is disclosed whereby a 5-50-nanometer-thick conformal tungsten coating can be formed over exposed semiconductor surfaces (e.g. silicon, germanium or silicon carbide) within a microelectromechanical (MEM) device for improved wear resistance and reliability. The tungsten coating is formed after cleaning the semiconductor surfaces to remove any organic material and oxide film from the surface. A final in situ cleaning step is performed by heating a substrate containing the MEM device to a temperature in the range of 200-600 .degree. C. in the presence of gaseous nitrogen trifluoride (NF.sub.3). The tungsten coating can then be formed by a chemical reaction between the semiconductor surfaces and tungsten hexafluoride (WF.sub.6) at an elevated temperature, preferably about 450.degree. C. The tungsten deposition process is self-limiting and covers all exposed semiconductor surfaces including surfaces in close contact. The present invention can be applied to many different types of MEM devices including microrelays, micromirrors and microengines. Additionally, the tungsten wear-resistant coating of the present invention can be used to enhance the hardness, wear resistance, electrical conductivity, optical reflectivity and chemical inertness of one or more semiconductor surfaces within a MEM device.

  2. Measurements of neutron-induced reactions in inverse kinematics and applications to nuclear astrophysics

    E-Print Network [OSTI]

    Reifarth, René; Endres, Anne; Göbel, Kathrin; Heftrich, Tanja; Glorius, Jan; Koloczek, Alexander; Sonnabend, Kerstin; Travaglio, Claudia; Weigand, Mario

    2015-01-01

    Neutron capture cross sections of unstable isotopes are important for neutron-induced nucleosynthesis as well as for technological applications. A combination of a radioactive beam facility, an ion storage ring and a high flux reactor would allow a direct measurement of neutron induced reactions over a wide energy range on isotopes with half lives down to minutes. The idea is to measure neutron-induced reactions on radioactive ions in inverse kinematics. This means, the radioactive ions will pass through a neutron target. In order to efficiently use the rare nuclides as well as to enhance the luminosity, the exotic nuclides can be stored in an ion storage ring. The neutron target can be the core of a research reactor, where one of the central fuel elements is replaced by the evacuated beam pipe of the storage ring. Using particle detectors and Schottky spectroscopy, most of the important neutron-induced reactions, such as (n,$\\gamma$), (n,p), (n,$\\alpha$), (n,2n), or (n,f), could be investigated.

  3. Thermal stability of intermetallic phases in Fe-rich Fe-Cr-Ni-Mo alloys

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Yang, Ying; Tan, Lizhen; Busby, Jeremy T.

    2015-06-12

    Understanding the stability of precipitate phases in the Fe-rich Fe-Cr-Ni-Mo alloys is critical to the alloy design and application of Mo-containing Austenitic steels. Coupled with thermodynamic modeling, stability of the chi and Laves phases in two Fe-Cr-Ni-Mo alloys were investigated at 1000, 850 and 700 °C for different annealing time. The morphologies, compositions and crystal structures of the matrix and precipitate phases were carefully examined by Scanning Electron Microscopy, Electron Probe Microanalysis, X-ray diffraction and Transmission Electron Microscopy. The two key findings resulted from this work. One is that the chi phase is stable at high temperature and transformed intomore »the Laves phase at low temperature. The other is that both the chi and Laves phases have large solubilites of Cr, Mo and Ni, among which the Mo solubility has a major role on the relative stability of the precipitate phases. The developed thermodynamic models were then applied to evaluating the Mo effect on the stability of precipitate phases in AISI 316 and NF709 alloys.« less

  4. Fresh Water Generation from Aquifer-Pressured Carbon Storage

    SciTech Connect (OSTI)

    Aines, R D; Wolery, T J; Bourcier, W L; Wolfe, T; Haussmann, C

    2010-02-19

    Can we use the pressure associated with sequestration to make brine into fresh water? This project is establishing the potential for using brine pressurized by Carbon Capture and Storage (CCS) operations in saline formations as the feedstock for desalination and water treatment technologies including reverse osmosis (RO) and nanofiltration (NF). Possible products are: Drinking water, Cooling water, and Extra aquifer space for CO{sub 2} storage. The conclusions are: (1) Many saline formation waters appear to be amenable to largely conventional RO treatment; (2) Thermodynamic modeling indicates that osmotic pressure is more limiting on water recovery than mineral scaling; (3) The use of thermodynamic modeling with Pitzer's equations (or Extended UNIQUAC) allows accurate estimation of osmotic pressure limits; (4) A general categorization of treatment feasibility is based on TDS has been proposed, in which brines with 10,000-85,000 mg/L are the most attractive targets; (5) Brines in this TDS range appear to be abundant (geographically and with depth) and could be targeted in planning future CCS operations (including site selection and choice of injection formation); and (6) The estimated cost of treating waters in the 10,000-85,000 mg/L TDS range is about half that for conventional seawater desalination, due to the anticipated pressure recovery.

  5. Multi-Gigabit Wireless data transfer at 60 GHz

    E-Print Network [OSTI]

    Soltveit, H K; Wiedner, D

    2012-01-01

    In this paper we describe the status of the first prototype of the 60 GHz wireless Multi-gigabit data transfer topology currently under development at University of Heidelberg using IBM 130 nm SiGe HBT BiCMOS technology. The 60 GHz band is very suitable for high data rate and short distance applications as for example needed in the HEP experments. The wireless transceiver consist of a transmitter and a receiver. The transmitter includes an On-Off Keying (OOK) modulator, an Local Oscillator (LO), a Power Amplifier (PA) and a BandPass Filter (BPF). The receiver part is composed of a BandPass- Filter (BPF), a Low Noise Amplifier (LNA), a double balanced down-convert Gilbert mixer, a Local Oscillator (LO), then a BPF to remove the mixer introduced noise, an Intermediate Amplifier (IF), an On-Off Keying demodulator and a limiting amplifier. The first prototype would be able to handle a data-rate of about 3.5 Gbps over a link distance of 1 m. The first simulations of the LNA show that a Noise Figure (NF) of 5 dB, a...

  6. Multi-Gigabit Wireless data transfer at 60 GHz

    E-Print Network [OSTI]

    H. K. Soltveit; R. Brenner. A. Schöning; D. Wiedner

    2012-06-11

    In this paper we describe the status of the first prototype of the 60 GHz wireless Multi-gigabit data transfer topology currently under development at University of Heidelberg using IBM 130 nm SiGe HBT BiCMOS technology. The 60 GHz band is very suitable for high data rate and short distance applications as for example needed in the HEP experments. The wireless transceiver consist of a transmitter and a receiver. The transmitter includes an On-Off Keying (OOK) modulator, an Local Oscillator (LO), a Power Amplifier (PA) and a BandPass Filter (BPF). The receiver part is composed of a BandPass- Filter (BPF), a Low Noise Amplifier (LNA), a double balanced down-convert Gilbert mixer, a Local Oscillator (LO), then a BPF to remove the mixer introduced noise, an Intermediate Amplifier (IF), an On-Off Keying demodulator and a limiting amplifier. The first prototype would be able to handle a data-rate of about 3.5 Gbps over a link distance of 1 m. The first simulations of the LNA show that a Noise Figure (NF) of 5 dB, a power gain of 21 dB at 60 GHz with a 3 dB bandwidth of more than 20 GHz with a power consumption 11 mW are achieved. Simulations of the PA show an output referred compression point P1dB of 19.7 dB at 60 GHz.

  7. Gradient flow and scale setting on MILC HISQ ensembles

    E-Print Network [OSTI]

    Bazavov, A; Brown, N; DeTar, C; Foley, J; Gottlieb, Steven; Heller, U M; Komijani, J; Laiho, J; Levkova, L; Sugar, R L; Toussaint, D; Van de Water, R S

    2015-01-01

    We report on a scale determination with gradient-flow techniques on the $N_f=2+1+1$ HISQ ensembles generated by the MILC collaboration. The ensembles include four lattice spacings, ranging from approximately 0.15 to 0.06 fm, and both physical and unphysical values of the quark masses. The scales $\\sqrt{t_0}/a$ and $w_0/a$ and their tree-level improvements, $\\sqrt{t_{0,{\\rm imp}}}$ and $w_{0,{\\rm imp}}$, are computed on each ensemble using Symanzik flow and the cloverleaf definition of the energy density $E$. Using a combination of continuum chiral perturbation theory and a Taylor-series ansatz for the lattice-spacing and strong-coupling dependence, the results are simultaneously extrapolated to the continuum and interpolated to physical quark masses. We determine the scales $\\sqrt{t_0} = 0.1416({}_{-5}^{+8})$ fm and $w_0 = 0.1717({}_{-11}^{+12})$ fm, where the errors are sums, in quadrature, of statistical and all systematic errors. The precision of $w_0$ and $\\sqrt{t_0}$ is comparable to or more precise than...

  8. Highly efficient pulsed power supply system with a two-stage LC generator and a step-up transformer for fast capillary discharge soft x-ray laser at shorter wavelength

    SciTech Connect (OSTI)

    Sakai, Yusuke; Takahashi, Shnsuke; Komatsu, Takanori; Song, Inho; Watanabe, Masato; Hotta, Eiki [Department of Energy Sciences, Tokyo Institute of Technology, 4259-J2-35, Nagatsuta, Midori-ku, Yokohama 226-8502 (Japan)

    2010-01-15

    Highly efficient and compact pulsed power supply system for a capillary discharge soft x-ray laser (SXRL) has been developed. The system consists of a 2.2 {mu}F two-stage LC inversion generator, a 2:54 step-up transformer, a 3 nF water capacitor, and a discharge section with a few tens of centimeter length capillary. Adoption of the pulsed transformer in combination with the LC inversion generator enables us to use only one gap switch in the circuit for charging the water capacitor up to about 0.5 MV. Furthermore, step-up ratio of a water capacitor voltage to a LC inversion generator initial charging voltage is about 40 with energy transfer efficiency of about 50%. It also leads to good reproducibility of a capillary discharge which is necessary for lasing a SXRL stably. For the study of the possibility of lasing a SXRL at shorter wavelength in a small laboratory scale, high-density and high-temperature plasma column suitable for the laser can be generated relatively easily with this system.

  9. Spent nuclear fuel recycling with plasma reduction and etching

    DOE Patents [OSTI]

    Kim, Yong Ho

    2012-06-05

    A method of extracting uranium from spent nuclear fuel (SNF) particles is disclosed. Spent nuclear fuel (SNF) (containing oxides of uranium, oxides of fission products (FP) and oxides of transuranic (TRU) elements (including plutonium)) are subjected to a hydrogen plasma and a fluorine plasma. The hydrogen plasma reduces the uranium and plutonium oxides from their oxide state. The fluorine plasma etches the SNF metals to form UF6 and PuF4. During subjection of the SNF particles to the fluorine plasma, the temperature is maintained in the range of 1200-2000 deg K to: a) allow any PuF6 (gas) that is formed to decompose back to PuF4 (solid), and b) to maintain stability of the UF6. Uranium (in the form of gaseous UF6) is easily extracted and separated from the plutonium (in the form of solid PuF4). The use of plasmas instead of high temperature reactors or flames mitigates the high temperature corrosive atmosphere and the production of PuF6 (as a final product). Use of plasmas provide faster reaction rates, greater control over the individual electron and ion temperatures, and allow the use of CF4 or NF3 as the fluorine sources instead of F2 or HF.

  10. The behavior of fission products during nuclear rocket reactor tests

    SciTech Connect (OSTI)

    Bokor, P.C.; Kirk, W.L.; Bohl, R.J.

    1991-01-01

    The experience base regarding fission product behavior developed during the Rover program, the nuclear rocket development program of 1955--1972, will be useful in planning a renewed nuclear rocket program. During the Rover program, 20 reactors were tested at the Nuclear Rocket Development Station in Nevada. Nineteen of these discharged effluent directly into the atmosphere; the last reactor tested, a non-flight-prototypic, fuel-element-testing reactor called the Nuclear Furnace (NF-1) was connected to an effluent cleanup system that removed fission products before the hydrogen coolant (propellant) was discharged to the atmosphere. In general, we are able to increase both test duration and fuel temperature during the test series. Therefore fission product data from the later part of the program are more interesting and more applicable to future reactors. We have collected fission product retention (and release) data reported in both formal and informal publications for six of the later reactor tests; five of these were Los Alamos reactors that were firsts of a kind in configuration or operating conditions. We have also, with the cooperation of Westinghouse, included fission product data from the NRX-A6 reactor, the final member of series of developmental reactors with the same basic geometry, but with significant design and fabrication improvements as the series continued. Table 1 lists the six selected reactors and the test parameters for each.

  11. Neutrino factory

    SciTech Connect (OSTI)

    Bogomilov, M.; Matev, R.; Tsenov, R.; Dracos, M.; Bonesini, M.; Palladino, V.; Tortora, L.; Mori, Y.; Planche, T.; Lagrange, J.?B.; Kuno, Y.; Benedetto, E.; Efthymiopoulos, I.; Garoby, R.; Gilardoini, S.; Martini, M.; Wildner, E.; Prior, G.; Blondel, A.; Karadzhow, Y.; Ellis, M.; Kyberd, P.; Bayes, R.; Laing, A.; Soler, F.?J.?P.; Alekou, A.; Apollonio, M.; Aslaninejad, M.; Bontoiu, C.; Jenner, L.?J.; Kurup, A.; Long, K.; Pasternak, J.; Zarrebini, A.; Poslimski, J.; Blackmore, V.; Cobb, J.; Tunnell, C.; Andreopoulos, C.; Bennett, J.?R.?J.; Brooks, S.; Caretta, O.; Davenne, T.; Densham, C.; Edgecock, T.?R.; Fitton, M.; Kelliher, D.; Loveridge, P.; McFarland, A.; Machida, S.; Prior, C.; Rees, G.; Rogers, C.; Rooney, M.; Thomason, J.; Wilcox, D.; Booth, C.; Skoro, G.; Back, J.?J.; Harrison, P.; Berg, J.?S.; Fernow, R.; Gallardo, J.?C.; Gupta, R.; Kirk, H.; Simos, N.; Stratakis, D.; Souchlas, N.; Witte, H.; Bross, A.; Geer, S.; Johnstone, C.; Makhov, N.; Neuffer, D.; Popovic, M.; Strait, J.; Striganov, S.; Morfín, J.?G.; Wands, R.; Snopok, P.; Bagacz, S.?A. [Thomas Jefferson National Accelerator Facility, Newport News, VA (United States); Morozov, V.; Roblin, Y.; Cline, D.; Ding, X.; Bromberg, C.; Hart, T.; Abrams, R.?J.; Ankenbrandt, C.?M.; Beard, K.?B.; Cummings, M.?A.?C.; Flanagan, G.; Johnson, R.?P.; Roberts, T.?J.; Yoshikawa, C.?Y.; Graves, V.?B.; McDonald, K.?T.; Coney, L.; Hanson, G.

    2014-12-01

    The properties of the neutrino provide a unique window on physics beyond that described by the standard model. The study of subleading effects in neutrino oscillations, and the race to discover CP-invariance violation in the lepton sector, has begun with the recent discovery that theta(13) > 0. The measured value of theta(13) is large, emphasizing the need for a facility at which the systematic uncertainties can be reduced to the percent level. The neutrino factory, in which intense neutrino beams are produced from the decay of muons, has been shown to outperform all realistic alternatives and to be capable of making measurements of the requisite precision. Its unique discovery potential arises from the fact that only at the neutrino factory is it practical to produce high-energy electron (anti) neutrino beams of the required intensity. This paper presents the conceptual design of the neutrino factory accelerator facility developed by the European Commission Framework Programme 7 EURO nu. Design Study consortium. EURO nu coordinated the European contributions to the International Design Study for the Neutrino Factory (the IDS-NF) collaboration. The EURO nu baseline accelerator facility will provide 10(21) muon decays per year from 12.6 GeV stored muon beams serving a single neutrino detector situated at a source-detector distance of between 1 500 km and 2 500 km. A suite of near detectors will allow definitive neutrino-scattering experiments to be performed.

  12. Gradient Flow Analysis on MILC HISQ Ensembles

    E-Print Network [OSTI]

    A. Bazavov; C. Bernard; N. Brown; C. DeTar; J. Foley; Steven Gottlieb; U. M. Heller; J. E. Hetrick; J. Komijani; J. Laiho; L. Levkova; M. Oktay; R. L. Sugar; D. Toussaint; R. S. Van de Water; R. Zhou

    2014-11-14

    We report on a preliminary scale determination with gradient-flow techniques on the $N_f = 2 + 1 + 1$ HISQ ensembles generated by the MILC collaboration. The ensembles include four lattice spacings, ranging from 0.15 to 0.06 fm, and both physical and unphysical values of the quark masses. The scales $\\sqrt{t_0}/a$ and $w_0/a$ are computed using Symanzik flow and the cloverleaf definition of $\\langle E \\rangle$ on each ensemble. Then both scales and the meson masses $aM_\\pi$ and $aM_K$ are adjusted for mistunings in the charm mass. Using a combination of continuum chiral perturbation theory and a Taylor series ansatz in the lattice spacing, the results are simultaneously extrapolated to the continuum and interpolated to physical quark masses. Our preliminary results are $\\sqrt{t_0} = 0.1422(7)$fm and $w_0 = 0.1732(10)$fm. We also find the continuum mass-dependence of $w_0$.

  13. MCNP Super Lattice Method for VHTR ORIGEN2.2 Nuclear Library Improvement Based on ENDF/B-VII

    SciTech Connect (OSTI)

    G. S. Chang; J. R. Parry

    2010-10-01

    The advanced Very High Temperature gas-cooled Reactor (VHTR) achieves simplification of safety through reliance on innovative features and passive systems. One of the VHTRs innovative features is the reliance on ceramic-coated fuel particles to retain the fission products under extreme accident conditions. The effect of the random fuel kernel distribution in the fuel prismatic block creates a double-heterogeneous lattice, which needs to be addressed through the use of the newly developed prismatic super Kernel-by-Kernel Fuel (KbKF) lattice model method. Based on the new ENDF/B-VII nuclear cross section evaluated data, the developed KbKF super lattice model was then used with MCNP to calculate the material isotopes neutron reaction rates, such as, (n,?); (n,n’); (n,2n’); (n,f); (n,p); (n,?). Then, the MCNP-calculated results are rearranged to generate a set of new libraries “VHTRXS.lib,” for the ORIGEN2.2 isotopes depletion and build-up analysis code. The libraries contain one group cross section data for the structural light elements, actinides, and fission products that can be applied in the VHTR related fuel burnup and material transmutation analysis codes. The efficiency and ease of use of the MCNP method to generate and update the ORIGEN2.2 one-group spectrum weighed cross section library for VHTR was demonstrated.

  14. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    SciTech Connect (OSTI)

    Greulich-Bode, Karin M.; Wang, Mei; Rhein, Andreas P.; Weier, Jingly F.; Weier, Heinz-Ulli G.

    2008-12-04

    Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements involving genomic intervals of less than 100kb, careful probe selection and characterization are of paramount importance. We describe a sensitive approach to quality control probe clones suspected of carrying deletions or for measuring clone overlap with near kilobase resolution. The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. Two examples demonstrate the application of this technique: mapping of a gene-specific {approx}6kb plasmid onto an unusually small, {approx}55kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-{kappa}B2 locus. The relatively simple method presented here does not require specialized equipment and may thus find widespread applications in DNA probe preparation and characterization, the assembly of physical maps for model organisms or in studies on gene rearrangements.

  15. Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

    SciTech Connect (OSTI)

    Greulich-Bode, Karin; Wang, Mei; Rhein, Andreas; Weier, Jingly; Weier, Heinz-Ulli

    2008-12-16

    Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements involving genomic intervals of less than 100kb, careful probe selection and characterization are of paramount importance. We describe a sensitive approach to quality control probe clones suspected of carrying deletions or for measuring clone overlap with near kilobase resolution. The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. Two examples demonstrate the application of this technique: mapping of a gene-specific {approx}6kb plasmid onto an unusually small, {approx}55kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-?B2 locus. The relatively simple method presented here does not require specialized equipment and may thus find widespread applications in DNA probe preparation and characterization, the assembly of physical maps for model organisms or in studies on gene rearrangements.

  16. The I=2 ?? S-wave Scattering Phase Shift from Lattice QCD

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Beane, S. R.; Chang, E.; Detmold, W.; Lin, H. W.; Luu, T. C.; Orginos, K.; Parreno, A.; Savage, M. J.; Torok, A.; Walker-Loud, A.

    2012-02-16

    The ?+?+ s-wave scattering phase-shift is determined below the inelastic threshold using Lattice QCD. Calculations were performed at a pion mass of m? ? 390 MeV with an anisotropic nf = 2+1 clover fermion discretization in four lattice volumes, with spatial extent L ? 2.0, 2.5, 3.0 and 3.9 fm, and with a lattice spacing of bs ? 0.123 fm in the spatial direction and bt bs/3.5 in the time direction. The phase-shift is determined from the energy-eigenvalues of ?+?+ systems with both zero and non-zero total momentum in the lattice volume using Luscher's method. Our calculations are precise enoughmore »to allow for a determination of the threshold scattering parameters, the scattering length a, the effective range r, and the shape-parameter P, in this channel and to examine the prediction of two-flavor chiral perturbation theory: m?2 a r = 3+O(m?2/??2). Chiral perturbation theory is used, with the Lattice QCD results as input, to predict the scattering phase-shift (and threshold parameters) at the physical pion mass. Our results are consistent with determinations from the Roy equations and with the existing experimental phase shift data.« less

  17. The depletion of Interleukin-8 causes cell cycle arrest and increases the efficacy of docetaxel in breast cancer cells

    SciTech Connect (OSTI)

    Shao, Nan; Chen, Liu-Hua; Ye, Run-Yi; Lin, Ying; Wang, Shen-Ming

    2013-02-15

    Highlights: ? IL-8 depletion affects cell cycle distribution. ? Intrinsic IL-8 mediates breast cancer cell migration and invasion. ? IL-8 siRNA down regulates key factors that control survival and metastatic pathway. ? IL-8 depletion reduces integrin ?3 expression. ? IL-8 depletion increases the chemosensitivity to docetaxel. -- Abstract: IL-8 is a multi-functional pro-inflammatory chemokine, which is highly expressed in cancers, such as ER-negative breast cancer. The present study demonstrates the pervasive role of IL-8 in the malignant progression of ER-negative breast cancer. IL-8 siRNA inhibited proliferation and delayed the G1 to S cell cycle progression in MDA-MB-231 and BT549 cells. IL-8 silencing resulted in the upregulation of the CDK inhibitor p27, the downregulation of cyclin D1, and the reduction of phosphorylated-Akt and NF-?B activities. IL-8 depletion also increased the chemosensitivity to docetaxel. These results indicate a role for IL-8 in promoting tumor cell survival and resistance to docetaxel and highlight the potential therapeutic significance of IL-8 depletion in ER-negative breast cancer patients.

  18. Combination of ascorbate/epigallocatechin-3-gallate/gemcitabine synergistically induces cell cycle deregulation and apoptosis in mesothelioma cells

    SciTech Connect (OSTI)

    Martinotti, Simona; Ranzato, Elia; Parodi, Monica; Vitale, Massimo; Burlando, Bruno

    2014-01-01

    Malignant mesothelioma (MMe) is a poor-prognosis tumor in need of innovative therapies. In a previous in vivo study, we showed synergistic anti-MMe properties of the ascorbate/epigallocatechin-3-gallate/gemcitabine combination. We have now focused on the mechanism of action, showing the induction of apoptosis and cell cycle arrest through measurements of caspase 3, intracellular Ca{sup 2+}, annexin V, and DNA content. StellArray™ PCR technology and Western immunoblotting revealed DAPK2-dependent apoptosis, upregulation of cell cycle promoters, downregulation of cell cycle checkpoints and repression of NF?B expression. The complex of data indicates that the mixture is synergistic in inducing cell cycle deregulation and non-inflammatory apoptosis, suggesting its possible use in MMe treatment. - Highlights: • Ascorbate/epigallocathechin-gallate/gemcitabine has been tested on mesothelioma cells • A synergistic mechanism has been shown for cell cycle arrest and apoptosis • PCR-array analysis has revealed the de-regulation of apoptosis and cell cycle genes • Maximum upregulation has been found for the Death-Associated Protein Kinase-2 gene • Data suggest that the mixture could be used as a clinical treatment.

  19. Cosmography of f(R) gravity

    E-Print Network [OSTI]

    Capozziello, S; Salzano, V

    2008-01-01

    It is nowadays accepted that the universe is undergoing a phase of accelerated expansion as tested by the Hubble diagram of Type Ia Supernovae (SNeIa) and several LSS observations. Future SNeIa surveys and other probes will make it possible to better characterize the dynamical state of the universe renewing the interest in cosmography which allows a model independent analysis of the distance - redshift relation. On the other hand, fourth order theories of gravity, also referred to as $f(R)$ gravity, have attracted a lot of interest since they could be able to explain the accelerated expansion without any dark energy. We show here how it is possible to relate the cosmographic parameters (namely the deceleration $q_0$, the jerk $j_0$, the snap $s_0$ and the lerk $l_0$ parameters) to the present day values of $f(R)$ and its derivatives $f^{(n)}(R) = d^nf/dR^n$ (with $n = 1, 2, 3$) thus offering a new tool to constrain such higher order models. Our analysis thus offers the possibility to relate the model independ...

  20. Cosmography of f(R) gravity

    E-Print Network [OSTI]

    S. Capozziello; V. F. Cardone; V. Salzano

    2008-07-08

    It is nowadays accepted that the universe is undergoing a phase of accelerated expansion as tested by the Hubble diagram of Type Ia Supernovae (SNeIa) and several LSS observations. Future SNeIa surveys and other probes will make it possible to better characterize the dynamical state of the universe renewing the interest in cosmography which allows a model independent analysis of the distance - redshift relation. On the other hand, fourth order theories of gravity, also referred to as $f(R)$ gravity, have attracted a lot of interest since they could be able to explain the accelerated expansion without any dark energy. We show here how it is possible to relate the cosmographic parameters (namely the deceleration $q_0$, the jerk $j_0$, the snap $s_0$ and the lerk $l_0$ parameters) to the present day values of $f(R)$ and its derivatives $f^{(n)}(R) = d^nf/dR^n$ (with $n = 1, 2, 3$) thus offering a new tool to constrain such higher order models. Our analysis thus offers the possibility to relate the model independent results coming from cosmography to the theoretically motivated assumptions of $f(R)$ cosmology.

  1. Norathyriol Suppresses Skin Cancers Induced by Solar Ultraviolet Radiation by Targeting ERK Kinases

    SciTech Connect (OSTI)

    Li, Jixia; Malakhova, Margarita; Mottamal, Madhusoodanan; Reddy, Kanamata; Kurinov, Igor; Carper, Andria; Langfald, Alyssa; Oi, Naomi; Kim, Myoung Ok; Zhu, Feng; Sosa, Carlos P.; Zhou, Keyuan; Bode, Ann M.; Dong, Zigang

    2012-06-27

    Ultraviolet (UV) irradiation is the leading factor in the development of skin cancer, prompting great interest in chemopreventive agents for this disease. In this study, we report the discovery of norathyriol, a plant-derived chemopreventive compound identified through an in silico virtual screening of the Chinese Medicine Library. Norathyriol is a metabolite of mangiferin found in mango, Hypericum elegans, and Tripterospermum lanceolatum and is known to have anticancer activity. Mechanistic investigations determined that norathyriol acted as an inhibitor of extracellular signal-regulated kinase (ERK)1/2 activity to attenuate UVB-induced phosphorylation in mitogen-activated protein kinases signaling cascades. We confirmed the direct and specific binding of norathyriol with ERK2 through a cocrystal structural analysis. The xanthone moiety in norathyriol acted as an adenine mimetic to anchor the compound by hydrogen bonds to the hinge region of the protein ATP-binding site on ERK2. Norathyriol inhibited in vitro cell growth in mouse skin epidermal JB6 P+ cells at the level of G{sub 2}-M phase arrest. In mouse skin tumorigenesis assays, norathyriol significantly suppressed solar UV-induced skin carcinogenesis. Further analysis indicated that norathyriol mediates its chemopreventive activity by inhibiting the ERK-dependent activity of transcriptional factors AP-1 and NF-{kappa}B during UV-induced skin carcinogenesis. Taken together, our results identify norathyriol as a safe new chemopreventive agent that is highly effective against development of UV-induced skin cancer.

  2. Radioactive targets for neutron-induced cross section measurements

    SciTech Connect (OSTI)

    Kronenberg, A. (Andreas); Bond, E. M. (Evelyn M.); Glover, S. E. (Samuel E.); Rundberg, R. S. (Robert S.); Vieira, D. J. (David J.); Esch, E. I. (Ernst-Ingo); Reifarth, R. (Rene); Ullmann, J. L. (John L.); Haight, Robert C.; Rochmann, D. (Dimitri)

    2004-01-01

    Measurements using radioactive targets are important for the determination of key reaction path ways associated with the synthesis of the elements in nuclear astrophysics (sprocess), advanced fuel cycle initiative (transmutation of radioactive waste), and stockpile stewardship. High precision capture cross-section measurements are needed to interpret observations, predict elemental or isotopical ratios, and unobserved abundances. There are two new detector systems that are presently being commissioned at Los Alamos National Laboratory for very precise measurements of (n,{gamma}) and (n,f) cross-sections using small quantities of radioactive samples. DANCE (Detector for Advanced Neutron-Capture Experiments), a 4 {pi} gamma array made up of 160 BaF{sub 2} detectors, is designed to measure neutron capture cross-sections of unstable nuclei in the low-energy range (thermal to {approx}500 keV). The high granularity and high detection efficiency of DANCE, combined with the high TOF-neutron flux available at the Lujan Center provides a versatile tool for measuring many important cross section data using radioactive and isotopically enriched targets of about 1 milligram. Another powerful instrument is the Lead-slowing down spectrometer (LSDS), which will enable the measurement of neutron-induced fission cross-section of U-235m and other short-lived actinides in a energy range from 1-200 keV with sample sizes down to 10 nanograms. Due to the short half-life of the U-235m isomer (T{sub 1/2} = 26 minutes), the samples must be rapidly and repeatedly extracted from its {sup 239}Pu parent. Since {sup 239}Pu is itself highly fissile, the separation must not only be rapid, but must also be of very high purity (the Pu must be removed from the U with a decontamination factor >10{sup 12}). Once extracted and purified, the {sup 235m}U isomer would be electrodeposited on solar cells as a fission detector and placed within the LSDS for direct (n,f) cross section measurements. The production of radioactive targets of a few milligrams will be described as well as the containment for safe handling of these targets at the Lujan Center at LANSCE. To avoid any contamination, the targets are electrochemically fixed onto thin Ti foils and two foils are placed back to back to contain the radioactive material within. This target sandwich is placed in a cylinder made of aluminum with thin translucent windows made of Kapton. Actinides targets, such as {sup 234,235,236,238}U, {sup 237}Np, and {sup 239}Pu are prepared by electrodeposition or molecular plating techniques. Target thicknesses of 1-2 mg/cm{sup 2} with sizes of 1 cm{sup 2} or more have been made. Other targets will be fabricated from separation of irradiated isotopically enriched targets, such as {sup 155}Eu from {sup 154}Sm,{sup 171}Tm from {sup 170}Er, and {sup 147}Pm from {sup 146}Nd, which has been irradiated in the high flux reactor at ILL, Grenoble. A radioactive sample isotope separator (RSIS) is in the process of being commissioned for the preparation of other radioactive targets. A brief summary of these experiments and the radioactive target preparation technique will be given.

  3. Evidence for Bolgiano-Obukhov scaling in rotating stratified turbulence using high-resolution direct numerical simulations

    SciTech Connect (OSTI)

    Rosenberg, Duane L; Pouquet, Dr. Annick; Mininni, Dr. Pablo D.; Marino, Dr. Raffaele

    2015-01-01

    We report results on rotating stratified turbulence in the absence of forcing, with large-scale isotropic initial conditions, using direct numerical simulations computed on grids of up to $4096^3$ points. The Reynolds and Froude numbers are respectively equal to $Re=5.4\\times 10^4$ and $Fr=0.0242$. The ratio of the Brunt-V\\"ais\\"al\\"a to the inertial wave frequency, $N/f$, is taken to be equal to 5, a choice appropriate to model the dynamics of the southern abyssal ocean at mid latitudes. This gives a global buoyancy Reynolds number $R_B=ReFr^2=32$, a value sufficient for some isotropy to be recovered in the small scales beyond the Ozmidov scale, but still moderate enough that the intermediate scales where waves are prevalent are well resolved. We concentrate on the large-scale dynamics and confirm that the Froude number based on a typical vertical length scale is of order unity, with strong gradients in the vertical. Two characteristic scales emerge from this computation, and are identified from sharp variations in the spectral distribution of either total energy or helicity. A spectral break is also observed at a scale at which the partition of energy between the kinetic and potential modes changes abruptly, and beyond which a Kolmogorov-like spectrum recovers. Large slanted layers are ubiquitous in the flow in the velocity and temperature fields, and a large-scale enhancement of energy is also observed, directly attributable to the effect of rotation.

  4. A PROPOSAL TO MEASURE THE CROSS SECTION OF THE SPACE STAR IN NEUTRON-DEUTERON BREAKUP IN A RECOIL GEOMETRY SETUP

    SciTech Connect (OSTI)

    Benjamin J. Crowe III

    2009-09-30

    Nucleon-deuteron (Nd) breakup is an important tool for obtaining a better understanding of three-nucleon (3N) dynamics and for developing meson exchange descriptions of nuclear systems. The kinematics of the nd breakup reaction enable observables to be studied in a variety of exit-channel configurations that show sensitivity to realistic nucleon-nucleon (NN) potential models and three-nucleon force (3NF) models. Rigorous 3N calculations give very good descriptions of most 3N reaction data. However, there are still some serious discrepancies between data and theory. The largest discrepancy observed between theory and data for nd breakup is for the cross section for the space-star configuration. This discrepancy is known as the “Space star Anomaly”. Several experimental groups have obtained results consistent with the “Space Star Anomaly”, but it is important to note that they all used essentially the same experimental setup and so their experimental results are subject to the same systematic errors. We propose to measure the space-star cross-section at the Triangle Universities Nuclear Laboratory (TUNL) using an experimental technique that is significantly different from the one used in previous breakup experiments. This technique has been used by a research group from the University of Bonn to measure the neutron-neutron scattering length. There are three possible scenarios for the outcome of this work: 1) the new data are consistent with previous measurements; 2) the new data are not in agreement with previous measurements, but are in agreement with theory; and 3) the new data are not in agreement with either theory or previous measurements. Any one of the three scenarios will provide valuable insight on the Space Star Anomaly.

  5. Weak Matrix Elements on the Lattice --- Circa 1995

    E-Print Network [OSTI]

    A. Soni

    1995-10-20

    Status of weak matrix elements is reviewed. In particular, $\\epspeps$, $\\bkastg$, $B_K$, $B_B$ and $B_{B_s}$ are discussed and the overall situation with respect to the lattice effort and some of its phenomenological implications are summarized. For $\\epspeps$ the need for the relevant matrix elements is stressed in view of the forthcoming improved experiments. For some of the operators, (e.g.\\ $O_6$), even bounds on their matrix elements would be very helpful. On $\\bkastg$, a constant behavior of $T_2$ appears disfavored although dependence of $T_2$ could, of course, be milder than a simple pole. Improved data is badly needed to settle this important issue firmly, especially in view of its ramification for extractions of $V_{td}$ from $B\\to\\rho\\gamma$. On $B_K$, the preliminary result from JLQCD appears to contradict Sharpe \\etal\\ JLQCD data seems to fit very well to linear $a$ dependence and leads to an appreciably lower value of $B_K$. Four studies of $B_K$ in the ``full'' ($n_f=2$) theory indicate very little quenching effects on $B_K$; the full theory value seems to be just a little less than the quenched result. Based on expectations from HQET, analysis of the $B$-parameter ($B_{h\\ell}$) for the heavy-light mesons via $B_{h\\ell}={}$constant${}+{}$constants$^\\prime/m_{h\\ell}$ is suggested. A summary of an illustrative sample of hadron matrix elements is given and constraints on CKM parameters (e.g.\\ $V_{td}/V_{ts}$), on the unitarity triangle and on $x_s/x_d$, emerging from the lattice calculations along with experimental results are briefly discussed. In quite a few cases, for the first time, some indication of quenching errors on weak matrix elements are now becoming available.

  6. Curcumin blocks interleukin (IL)-2 signaling in T-lymphocytes by inhibiting IL-2 synthesis, CD25 expression, and IL-2 receptor signaling

    SciTech Connect (OSTI)

    Forward, Nicholas A.; Conrad, David M.; Power Coombs, Melanie R.; Doucette, Carolyn D.; Furlong, Suzanne J.; Lin, Tong-Jun; Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia ; Hoskin, David W.

    2011-04-22

    Highlights: {yields} Curcumin inhibits CD4{sup +} T-lymphocyte proliferation. {yields} Curcumin inhibits interleukin-2 (IL-2) synthesis and CD25 expression by CD4{sup +} T-lymphocytes. {yields} Curcumin interferes with IL-2 receptor signaling by inhibiting JAK3 and STAT5 phosphorylation. {yields} IL-2-dependent regulatory T-lymphocyte function and Foxp3 expression is downregulated by curcumin. -- Abstract: Curcumin (diferulomethane) is the principal curcuminoid in the spice tumeric and a potent inhibitor of activation-induced T-lymphocyte proliferation; however, the molecular basis of this immunosuppressive effect has not been well studied. Here we show that micromolar concentrations of curcumin inhibited DNA synthesis by mouse CD4{sup +} T-lymphocytes, as well as interleukin-2 (IL-2) and CD25 ({alpha} chain of the high affinity IL-2 receptor) expression in response to antibody-mediated cross-linking of CD3 and CD28. Curcumin acted downstream of protein kinase C activation and intracellular Ca{sup 2+} release to inhibit I{kappa}B phosphorylation, which is required for nuclear translocation of the transcription factor NF{kappa}B. In addition, IL-2-dependent DNA synthesis by mouse CTLL-2 cells, but not constitutive CD25 expression, was impaired in the presence of curcumin, which demonstrated an inhibitory effect on IL-2 receptor (IL-2R) signaling. IL-2-induced phosphorylation of STAT5A and JAK3, but not JAK1, was diminished in the presence of curcumin, indicating inhibition of critical proximal events in IL-2R signaling. In line with the inhibitory action of curcumin on IL-2R signaling, pretreatment of CD4{sup +}CD25{sup +} regulatory T-cells with curcumin downregulated suppressor function, as well as forkhead box p3 (Foxp3) expression. We conclude that curcumin inhibits IL-2 signaling by reducing available IL-2 and high affinity IL-2R, as well as interfering with IL-2R signaling.

  7. Production of Medical Radioisotopes with High Specific Activity in Photonuclear Reactions with $?$ Beams of High Intensity and Large Brilliance

    E-Print Network [OSTI]

    D. Habs; U. Köster

    2010-09-08

    We study the production of radioisotopes for nuclear medicine in $(\\gamma,x{\\rm n}+y{\\rm p})$ photonuclear reactions or ($\\gamma,\\gamma'$) photoexcitation reactions with high flux [($10^{13}-10^{15}$)$\\gamma$/s], small diameter $\\sim (100 \\, \\mu$m$)^2$ and small band width ($\\Delta E/E \\approx 10^{-3}-10^{-4}$) $\\gamma$ beams produced by Compton back-scattering of laser light from relativistic brilliant electron beams. We compare them to (ion,$x$n$ + y$p) reactions with (ion=p,d,$\\alpha$) from particle accelerators like cyclotrons and (n,$\\gamma$) or (n,f) reactions from nuclear reactors. For photonuclear reactions with a narrow $\\gamma$ beam the energy deposition in the target can be managed by using a stack of thin target foils or wires, hence avoiding direct stopping of the Compton and pair electrons (positrons). $(\\gamma,\\gamma')$ isomer production via specially selected $\\gamma$ cascades allows to produce high specific activity in multiple excitations, where no back-pumping of the isomer to the ground state occurs. We discuss in detail many specific radioisotopes for diagnostics and therapy applications. Photonuclear reactions with $\\gamma$ beams allow to produce certain radioisotopes, e.g. $^{47}$Sc, $^{44}$Ti, $^{67}$Cu, $^{103}$Pd, $^{117m}$Sn, $^{169}$Er, $^{195m}$Pt or $^{225}$Ac, with higher specific activity and/or more economically than with classical methods. This will open the way for completely new clinical applications of radioisotopes. For example $^{195m}$Pt could be used to verify the patient's response to chemotherapy with platinum compounds before a complete treatment is performed. Also innovative isotopes like $^{47}$Sc, $^{67}$Cu and $^{225}$Ac could be produced for the first time in sufficient quantities for large-scale application in targeted radionuclide therapy.

  8. Ellagic acid protects endothelial cells from oxidized low-density lipoprotein-induced apoptosis by modulating the PI3K/Akt/eNOS pathway

    SciTech Connect (OSTI)

    Ou, Hsiu-Chung [Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan (China); Lee, Wen-Jane [Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan (China); Tunghai University, Taichung, Taiwan (China); Lee, Shin-Da [Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan (China); Department of Healthcare Administration, Asia University, Taichung, Taiwan (China); Huang, Chih-Yang [Graduate Institute of Basic Medical Science, China Medical University, Taiwan (China); Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan (China); Chiu, Tsan-Hung [Department of Obstetrics and Gynecology, China Medical University Hospital, Taiwan (China); Tsai, Kun-Ling [Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan (China); Hsu, Wen-Cheng [Department of Metabolism and Endocrinology, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan (China); Sheu, Wayne Huey-Herng, E-mail: whhsheu@mail.cmu.edu.t [Department of Internal Medicine, Division of Endocrinology and Metabolism, Taichung Veterans General Hospital, Taichung, Taiwan (China); Department of Medicine, Chung Shan Medical University, Taichung, Taiwan (China); Institute of Medical Technology, National Chung-Hsing University, Taichung, Taiwan (China); College of Medicine, National Yang Ming University School of Medicine, Taipei, Taiwan (China)

    2010-10-15

    Endothelial apoptosis is a driving force in atherosclerosis development. Oxidized low-density lipoprotein (oxLDL) promotes inflammatory and thrombotic processes and is highly atherogenic, as it stimulates macrophage cholesterol accumulation and foam cell formation. Previous studies have shown that the phosphatidylinositol 3-kinase/Akt/endothelial nitric oxide synthase/nitric oxide (PI3K/Akt/eNOS/NO) pathway is involved in oxLDL-induced endothelial apoptosis. Ellagic acid, a natural polyphenol found in berries and nuts, has in recent years been the subject of intense research within the fields of cancer and inflammation. However, its protective effects against oxLDL-induced injury in vascular endothelial cells have not been clarified. In the present study, we investigated the anti-apoptotic effect of ellagic acid in human umbilical vein endothelial cells (HUVECs) exposed to oxLDL and explored the possible mechanisms. Our results showed that pretreatment with ellagic acid (5-20 {mu}M) significantly attenuated oxLDL-induced cytotoxicity, apoptotic features, and generation of reactive oxygen species (ROS). In addition, the anti-apoptotic effect of ellagic acid was partially inhibited by a PI3K inhibitor (wortmannin) and a specific eNOS inhibitor (cavtratin) but not by an ERK inhibitor (PD98059). In exploring the underlying mechanisms of ellagic acid action, we found that oxLDL decreased Akt and eNOS phosphorylation, which in turn activated NF-{kappa}B and downstream pro-apoptotic signaling events including calcium accumulation, destabilization of mitochondrial permeability, and disruption of the balance between pro- and anti-apoptotic Bcl-2 proteins. Those alterations induced by oxLDL, however, were attenuated by pretreatment with ellagic acid. The inhibition of oxLDL-induced endothelial apoptosis by ellagic acid is due at least in part to its anti-oxidant activity and its ability to modulate the PI3K/Akt/eNOS signaling pathway.

  9. Theoretical analyses of (n,xn) reactions on sup 235 U, sup 238 U, sup 237 Np, and sup 239 Pu for ENDF/B-VI

    SciTech Connect (OSTI)

    Young, P.G.; Arthur, E.D.

    1991-01-01

    Theoretical analyses were performed of neutron-induced reactions on {sup 235}U, {sup 238}U, {sup 237}Np, and {sup 239}Pu between 0.01 and 20 MeV in order to calculate neutron emission cross sections and spectra for ENDF/B-VI evaluations. Coupled-channel optical model potentials were obtained for each target nucleus by fitting total, elastic, and inelastic scattering cross section data, as well as low-energy average resonance data. The resulting deformed optical model potentials were used to calculate direct (n,n{prime}) cross sections and transmission coefficients for use in Hauser-Feshbach statistical theory analyses. A fission model with multiple barrier representation, width fluctuation corrections, and preequilibrium corrections were included in the analyses. Direct cross sections for higher-lying vibrational states were calculated using DWBA theory, normalized using B(E{ell}) values determined from (d,d{prime}) and Coulomb excitation data, where available, and from systematics otherwise. Initial fission barrier parameters and transition state density enhancements appropriate to the compound systems involved were obtained from previous analyses, especially fits to charged-particle fission probability data. The parameters for the fission model were adjusted for each target system to obtain optimum agreement with direct (n,f) cross section measurements, taking account of the various multichance fission channels, that is, the different compound systems involved. The results from these analyses were used to calculate most of the neutron (n,n), (n,n{prime}), and (n,xn) cross section data in the ENDF/B/VI evaluations for the above nuclei, and all of the energy-angle correlated spectra. The deformed optical model and fission model parameterizations are described. Comparisons are given between the results of these analyses and the previous ENDF/B-V evaluations as well as with the available experimental data. 14 refs., 3 figs., 1 tab.

  10. Expression of orphan G-protein coupled receptor GPR174 in CHO cells induced morphological changes and proliferation delay via increasing intracellular cAMP

    SciTech Connect (OSTI)

    Sugita, Kazuya; Yamamura, Chiaki; Tabata, Ken-ichi [Laboratory of Pharmacoinformatics, Graduate School of Ritsumeikan University, Kusatsu, Shiga 525-8577 (Japan)] [Laboratory of Pharmacoinformatics, Graduate School of Ritsumeikan University, Kusatsu, Shiga 525-8577 (Japan); Fujita, Norihisa, E-mail: nori@ph.ritsumei.ac.jp [Laboratory of Pharmacoinformatics, Graduate School of Ritsumeikan University, Kusatsu, Shiga 525-8577 (Japan) [Laboratory of Pharmacoinformatics, Graduate School of Ritsumeikan University, Kusatsu, Shiga 525-8577 (Japan); School of Pharmacy, Ristumeikan University, Kusatsu, Shiga 525-8577 (Japan)

    2013-01-04

    Highlights: Black-Right-Pointing-Pointer Expression of GPR174 in CHO cells induces morphological changes and proliferation delay. Black-Right-Pointing-Pointer These are due to increase in intracellular cAMP concentration. Black-Right-Pointing-Pointer Lysophosphatidylserine was identified to stimulate GPR174 leading to activate ACase. Black-Right-Pointing-Pointer The potencies of fatty acid moiety on LysoPS were oleoyl Greater-Than-Or-Slanted-Equal-To stearoyl > palmitoyl. Black-Right-Pointing-Pointer We propose that GPR174 is a lysophosphatidylserine receptor. -- Abstract: We established cell lines that stably express orphan GPCR GPR174 using CHO cells, and studied physiological and pharmacological features of the receptor. GPR174-expressing cells showed cell-cell adhesion with localization of actin filaments to cell membrane, and revealed significant delay of cell proliferation. Since the morphological changes of GPR174-cells were very similar to mock CHO cells treated with cholera toxin, we measured the concentration of intracellular cAMP. The results showed the concentration was significantly elevated in GPR174-cells. By measuring intracellular cAMP concentration in GPR174-cells, we screened lipids and nucleotides to identify ligands for GPR174. We found that lysophosphatidylserine (LysoPS) stimulated increase in intracellular cAMP in a dose-dependent manner. Moreover, phosphorylation of Erk was elevated by LysoPS in GPR174 cells. These LysoPS responses were inhibited by NF449, an inhibitor of G{alpha}{sub s} protein. These results suggested that GPR174 was a putative LysoPS receptor conjugating with G{alpha}{sub s}, and its expression induced morphological changes in CHO cells by constitutively activating adenylyl cycles accompanied with cell conjunctions and delay of proliferation.

  11. The Bayesian reconstruction of the in-medium heavy quark potential from lattice QCD and its stability

    E-Print Network [OSTI]

    Yannis Burnier; Olaf Kaczmarek; Alexander Rothkopf

    2014-11-12

    We report recent results of a non-perturbative determination of the static heavy-quark potential in quenched and dynamical lattice QCD at finite temperature. The real and imaginary part of this complex quantity are extracted from the spectral function of Wilson line correlators in Coulomb gauge. To obtain spectral information from Euclidean time numerical data, our study relies on a novel Bayesian prescription that differs from the Maximum Entropy Method. We perform simulations on quenched $32^3\\times N_\\tau$ $(\\beta=7.0,\\xi=3.5)$ lattices with $N_\\tau=24,...,96$, which cover $839{\\rm MeV} \\geq T\\geq 210 {\\rm MeV}$. To investigate the potential in a quark-gluon plasma with light u,d and s quarks we utilize $N_f=2+1$ ASQTAD lattices with $m_l=m_s/20$ by the HotQCD collaboration, giving access to temperatures between $286 {\\rm MeV} \\geq T\\geq 148{\\rm MeV}$. The real part of the potential exhibits a clean transition from a linear, confining behavior in the hadronic phase to a Debye screened form above deconfinement. Interestingly its values lie close to the color singlet free energies in Coulomb gauge at all temperatures. We estimate the imaginary part on quenched lattices and find that it is of the same order of magnitude as in hard-thermal loop perturbation theory. From among all the systematic checks carried out in our study, we discuss explicitly the dependence of the result on the default model and the number of datapoints.

  12. Evaluation of the ²³?Pu prompt fission neutron spectrum induced by neutrons of 500 keV and associated covariances

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Neudecker, D.; Talou, P.; Kawano, T.; Smith, D. L.; Capote, R.; Rising, M. E.; Kahler, A. C.

    2015-08-01

    We present evaluations of the prompt fission neutron spectrum (PFNS) of ²³?Pu induced by 500 keV neutrons, and associated covariances. In a previous evaluation by Talou et al. 2010, surprisingly low evaluated uncertainties were obtained, partly due to simplifying assumptions in the quantification of uncertainties from experiment and model. Therefore, special emphasis is placed here on a thorough uncertainty quantification of experimental data and of the Los Alamos model predicted values entering the evaluation. In addition, the Los Alamos model was extended and an evaluation technique was employed that takes into account the qualitative differences between normalized model predicted valuesmore »and experimental shape data. These improvements lead to changes in the evaluated PFNS and overall larger evaluated uncertainties than in the previous work. However, these evaluated uncertainties are still smaller than those obtained in a statistical analysis using experimental information only, due to strong model correlations. Hence, suggestions to estimate model defect uncertainties are presented, which lead to more reasonable evaluated uncertainties. The calculated keff of selected criticality benchmarks obtained with these new evaluations agree with each other within their uncertainties despite the different approaches to estimate model defect uncertainties. The keff one standard deviations overlap with some of those obtained using ENDF/B-VII.1, albeit their mean values are further away from unity. Spectral indexes for the Jezebel critical assembly calculated with the newly evaluated PFNS agree with the experimental data for selected (n,?) and (n,f) reactions, and show improvements for high-energy threshold (n,2n) reactions compared to ENDF/B-VII.1.« less

  13. Contrasting role of phospholipase C-{gamma}1 in the expression of immediate early genes induced by epidermal or platelet-derived growth factors

    SciTech Connect (OSTI)

    Liao Hongjun [Department of Biochemistry, Vanderbilt University School of Medicine, 606 Light Hall, Nashville, TN 37232-0146 (United States); Santos, Josue de los [Department of Biochemistry, Vanderbilt University School of Medicine, 606 Light Hall, Nashville, TN 37232-0146 (United States); Carpenter, Graham [Department of Biochemistry, Vanderbilt University School of Medicine, 606 Light Hall, Nashville, TN 37232-0146 (United States)]. E-mail: graham.carpenter@vanderbilt.edu

    2006-04-01

    While significant progress has been achieved in identifying the signal transduction elements that operate downstream of activated receptor tyrosine kinases, it remains unclear how different receptors utilize these signaling elements to achieve a common response. This study compares the capacity of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) to elicit the induction of immediate early gene (IEG) mRNAs in the presence or absence of phospholipase C-{gamma}1 (PLC-{gamma}1). The results show that while PDGF induction of nearly all IEG mRNAs is abrogated in plcg1 null cells, EGF induction of the same genes is variable in the null cells and exhibits three distinct responses. Five IEG mRNAs (Nup475, Cyr61, TF, Gly, TS7) are completely inducible by EGF in the presence or absence of PLC-{gamma}1, while three others (JE, KC, FIC) exhibit a stringent requirement for the presence of PLC-{gamma}1. The third type of response is exhibited by c-fos and COX-2. While these mRNAs are completely induced by EGF in the absence of PLC-{gamma}1, the time course of their accumulation is significantly delayed. No IEG was identified as completely inducible by EGF and PDGF in the absence of PLC-{gamma}1. Electrophoretic mobility shift assays (EMSA) demonstrate that PLC-{gamma}1 is necessary for nuclear extracts from PDGF-treated cells, but not EGF-treated cells, to interact with probes for AP-1 or NF-{kappa}B.

  14. Targeting NRF2 signaling for cancer chemoprevention

    SciTech Connect (OSTI)

    Kwak, Mi-Kyoung; Kensler, Thomas W.

    2010-04-01

    Modulation of the metabolism and disposition of carcinogens through induction of cytoprotective enzymes is one of several promising strategies to prevent cancer. Chemopreventive efficacies of inducers such as dithiolethiones and sulforaphane have been extensively studied in animals as well as in humans. The KEAP1-NRF2 system is a key, but not unilateral, molecular target for these chemopreventive agents. The transcription factor NRF2 (NF-E2-related factor 2) is a master regulator of the expression of a subset of genes, which produce proteins responsible for the detoxication of electrophiles and reactive oxygen species as well as the removal or repair of some of their damage products. It is believed that chemopreventive enzyme inducers affect the interaction between KEAP1 and NRF2 through either mediating conformational changes of the KEAP1 protein or activating phosphorylation cascades targeting the KEAP1-NRF2 complex. These events in turn affect NRF2 stability and trafficking. Recent advances elucidating the underlying structural biology of KEAP1-NRF2 signaling and identification of the gene clusters under the transcriptional control of NRF2 are facilitating understanding of the potential pleiotropic effects of NRF2 activators and discovery of novel classes of potent chemopreventive agents such as the triterpenoids. Although there is appropriately a concern regarding a deleterious role of the KEAP1-NRF2 system in cancer cell biology, especially as the pathway affects cell survival and drug resistance, the development and the use of NRF2 activators as chemopreventive agents still holds a great promise for protection of normal cells from a diversity of environmental stresses that contribute to the burden of cancer and other chronic, degenerative diseases.

  15. Radiological Impact Associated to Technologically Enhanced Naturally Occurring Radioactive Materials (TENORM) from Coal-Fired Power Plants Emissions - 13436

    SciTech Connect (OSTI)

    Dinis, Maria de Lurdes; Fiuza, Antonio; Soeiro de Carvalho, Jose; Gois, Joaquim [Geo-Environment and Resources Research Centre (CIGAR), Porto University, Faculty of Engineering - FEUP, Rua Dr. Roberto Frias, 4200-465 Porto (Portugal)] [Geo-Environment and Resources Research Centre (CIGAR), Porto University, Faculty of Engineering - FEUP, Rua Dr. Roberto Frias, 4200-465 Porto (Portugal); Meira Castro, Ana Cristina [School of Engineering Polytechnic of Porto - ISEP, Rua Dr. Antonio Bernardino de Almeida, 431, 4200-072, Porto (Portugal)] [School of Engineering Polytechnic of Porto - ISEP, Rua Dr. Antonio Bernardino de Almeida, 431, 4200-072, Porto (Portugal)

    2013-07-01

    Certain materials used and produced in a wide range of non-nuclear industries contain enhanced activity concentrations of natural radionuclides. In particular, electricity production from coal is one of the major sources of increased human exposure to naturally occurring radioactive materials. A methodology was developed to assess the radiological impact due to natural radiation background. The developed research was applied to a specific case study, the Sines coal-fired power plant, located in the southwest coastline of Portugal. Gamma radiation measurements were carried out with two different instruments: a sodium iodide scintillation detector counter (SPP2 NF, Saphymo) and a gamma ray spectrometer with energy discrimination (Falcon 5000, Canberra). Two circular survey areas were defined within 20 km of the power plant. Forty relevant measurements points were established within the sampling area: 15 urban and 25 suburban locations. Additionally, ten more measurements points were defined, mostly at the 20-km area. The registered gamma radiation varies from 20 to 98.33 counts per seconds (c.p.s.) corresponding to an external gamma exposure rate variable between 87.70 and 431.19 nGy/h. The highest values were measured at locations near the power plant and those located in an area within the 6 and 20 km from the stacks. In situ gamma radiation measurements with energy discrimination identified natural emitting nuclides as well as their decay products (Pb-212, Pb-2142, Ra-226, Th-232, Ac-228, Th-234, Pa-234, U- 235, etc.). According to the results, an influence from the stacks emissions has been identified both qualitatively and quantitatively. The developed methodology accomplished the lack of data in what concerns to radiation rate in the vicinity of Sines coal-fired power plant and consequently the resulting exposure to the nearby population. (authors)

  16. Maternal exposure to cadmium during gestation perturbs the vascular system of the adult rat offspring

    SciTech Connect (OSTI)

    Ronco, Ana Maria, E-mail: amronco@inta.cl [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile); Montenegro, Marcela; Castillo, Paula; Urrutia, Manuel [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile); Saez, Daniel [Faculty of Veterinary Medicine, University of Chile, Casilla 138-11, Santiago (Chile); Hirsch, Sandra [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile); Zepeda, Ramiro [Faculty of Medicine, University of Chile, Casilla 138-11, Santiago (Chile); Llanos, Miguel N. [Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Casilla 138-11, Santiago (Chile)

    2011-03-01

    Several cardiovascular diseases (CVD) observed in adulthood have been associated with environmental influences during fetal growth. Here, we show that maternal exposure to cadmium, a ubiquitously distributed heavy metal and main component of cigarette smoke is able to induce cardiovascular morpho-functional changes in the offspring at adult age. Heart morphology and vascular reactivity were evaluated in the adult offspring of rats exposed to 30 ppm of cadmium during pregnancy. Echocardiographic examination shows altered heart morphology characterized by a concentric left ventricular hypertrophy. Also, we observed a reduced endothelium-dependent reactivity in isolated aortic rings of adult offspring, while endothelium-independent reactivity remained unaltered. These effects were associated with an increase of hem-oxygenase 1 (HO-1) expression in the aortas of adult offspring. The expression of HO-1 was higher in females than males, a finding likely related to the sex-dependent expression of the vascular cell adhesion molecule 1 (VCAM-1), which was lower in the adult female. All these long-term consequences were observed along with normal birth weights and absence of detectable levels of cadmium in fetal and adult tissues of the offspring. In placental tissues however, cadmium levels were detected and correlated with increased NF-{kappa}B expression - a transcription factor sensitive to inflammation and oxidative stress - suggesting a placentary mechanism that affect genes related to the development of the cardiovascular system. Our results provide, for the first time, direct experimental evidence supporting that exposure to cadmium during pregnancy reprograms cardiovascular development of the offspring which in turn may conduce to a long term increased risk of CVD.

  17. Taurine protects methamphetamine-induced developmental angiogenesis defect through antioxidant mechanism

    SciTech Connect (OSTI)

    Shao, Xue; Hu, Zhengtao; Hu, Chunyan; Bu, Qian; Yan, Guangyan [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China)] [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China); Deng, Pengchi [Analytical and Testing Center, Sichuan University, Chengdu 610041 (China)] [Analytical and Testing Center, Sichuan University, Chengdu 610041 (China); Lv, Lei [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China)] [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China); Wu, Dan [College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041 (China)] [College of Basic and Forensic Medicine, Sichuan University, Chengdu 610041 (China); Deng, Yi; Zhao, Jinxuan; Zhu, Ruiming; Li, Yan; Li, Hongyu; Xu, Youzhi; Yang, Hanshuo; Zhao, Yinglan [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China)] [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China); Cen, Xiaobo, E-mail: xbcenalan@vip.sina.com [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China)] [National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041 (China)

    2012-05-01

    Investigations have characterized addictive drug-induced developmental cardiovascular malformation in human, non-human primate and rodent. However, the underlying mechanism of malformation caused by drugs during pregnancy is still largely unknown, and preventive and therapeutic measures have been lacking. Using {sup 1}H NMR spectroscopy, we profiled the metabolites from human embryo endothelial cells exposed to methamphetamine (METH) and quantified a total of 226 peaks. We identified 11 metabolites modified robustly and found that taurine markedly increased. We then validated the hypothesis that this dramatic increase in taurine could attribute to its effect in inhibiting METH-induced developmental angiogenesis defect. Taurine supplement showed a more significant potential than other metabolites in protecting against METH-induced injury in endothelial cells. Taurine strongly attenuated METH-induced inhibition of proliferation and migration in endothelial cells. Furthermore, death rate and vessel abnormality of zebrafish embryos treated with METH were greatly reversed by taurine. In addition, taurine supplement caused a rapid decrease in reactive oxygen species generation and strongly attenuated the excitable arise of antioxidase activities in the beginning of METH exposure prophase. Dysregulations of NF-?B, p-ERK as well as Bax, which reflect apoptosis, cell cycle arrest and oxidative stress in vascular endothelium, were blocked by taurine. Our results provide the first evidence that taurine prevents METH-caused developmental angiogenesis defect through antioxidant mechanism. Taurine could serve as a potential therapeutic or preventive intervention of developmental vascular malformation for the pregnant women with drug use. Highlights: ? Metabonomics findings. ? Abnormal development. ? Dysregulations of key proteins.

  18. Thermal aging modeling and validation on the Mo containing Fe-Cr-Ni alloys

    SciTech Connect (OSTI)

    Yang, Ying; Tan, Lizhen; Busby, Jeremy T.

    2015-04-01

    Thermodynamics of intermetallic phases in Fe-rich Fe-Cr-Ni-Mo alloys is critical knowledge to understand thermal aging effect on the phase stability of Mo-containing austenitic steels, which subsequently facilitates alloy design/improvement and degradation mitigation of these materials for reactor applications. Among the intermetallic phases, Chi (?), Laves, and Sigma (?) are often of concern because of their tendency to cause embrittlement of the materials. The focus of this study is thermal stability of the Chi and Laves phases as they were less studied compared to the Sigma phase. Coupled with thermodynamic modeling, thermal stability of intermetallic phases in Mo containing Fe-Cr-Ni alloys was investigated at 1000, 850 and 700 C for different annealing times. The morphologies, compositions and crystal structures of the precipitates of the intermetallic phases were carefully examined by scanning electron microscopy, electron probe microanalysis, X-ray diffraction, and transmission electron microscopy. Three key findings resulted from this study. First, the Chi phase is stable at high temperature, and with decreasing temperature it transforms into the Laves phase that is stable at low temperature. Secondly, Cr, Mo, Ni are soluble in both the Chi and Laves phases, with the solubility of Mo playing a major role in the relative stability of the intermetallic phases. Thirdly, in situ transformation from Chi phase to Laves phase was directly observed, which increased the local strain field, generated dislocations in the intermetallic phases, and altered the precipitate phase orientation relationship with the austenitic matrix. The thermodynamic models that were developed and validated were then applied to evaluating the effect of Mo on the thermal stability of intermetallic phases in type 316 and NF709 stainless steels.

  19. Polymer segregation under confinement: Free energy calculations and segregation dynamics simulations

    E-Print Network [OSTI]

    James M. Polson; Logan G. Montgomery

    2014-10-09

    Monte Carlo simulations are used to study the behavior of two polymers under confinement in a cylindrical tube. Each polymer is modeled as a chain of hard spheres. We measure the free energy of the system, F, as a function of the distance between the centers of mass of the polymers, lambda, and examine the effects on the free energy functions of varying the channel diameter D and length L, as well as the polymer length N and bending rigidity, kappa. For infinitely long cylinders, F is a maximum at lambda=0, and decreases with lambda until the polymers are no longer in contact. For flexible chains, the polymers overlap along the cylinder for low lambda, while above some critical value of lambda they are longitudinally compressed and non-overlapping while still in contact. We find that the free energy barrier height, scales as Delta F/k_BT~ND^{-1.93+/-0.01}. In addition, the overlap free energy scales as F/k_BT=Nf(lambda/N;D), where f is a function parameterized by D. For channels of finite L, the free energy barrier height increases with increasing confinement aspect ratio L/D at fixed volume fraction phi, and it decreases with increasing phi at fixed L/D. Increasing the polymer bending rigidity kappa monotonically reduces the overlap free energy. For strongly confined systems, F varies linearly with lambda with a slope that scales as F'(lambda)~-k_BT D^{-beta} P^{-alpha}, where beta approx 2 and alpha approx 0.37 for N=200 chains. These exponent values deviate slightly from those predicted using a simple model, possibly due to insufficiently satisfying the conditions defining the Odijk regime. Finally, we use Monte Carlo dynamics simulations to examine polymer segregation dynamics for fully flexible chains and observe segregation rates that decrease with decreasing entropic force magnitude. The polymers are not conformationally relaxed at later times during segregation.

  20. Studies of nonlinear electrodynamics of high-temperature superconductors

    SciTech Connect (OSTI)

    Lam, Quan-Chiu H.

    1991-08-01

    Nonlinear electrodynamics of high-{Tc} superconductors are studied both theoretically and experimentally. For powdered samples, a novel model is presented in which the metallographically observed superconducting grains in the powder are modeled as superconducting current loops of various areas with weak links. Surprising harmonic generation behavior in an arc field, H{sub 1} cos({omega}t), is predicted by the model; the power at high harmonics show sharp dips almost periodic in a superposing dc magnetic field, revealing flux quantization in the prototype loops in the model. Such oscillation of the harmonic power in dc magnetic field P{sub nf}(H{sub dc}), is indeed experimentally observed in powdered YBa{sub 2}Cu{sub 3}O{sub 7}. Other experimental aspects also agree with model predictions. For bulk sintered cylindrical samples, a generalized critical state model is presented. In this model, the nonlinear electrodynamics are due to flux-pinning, somewhat similar to low-temperature type-II superconductors, but with a more generalized critical current densities' dependence on magnetic field -- J{sub c}(H){approximately}H{sub local}{sup -{beta}}, with {beta} being an adjustable parameter. Experiments in ac and dc magnetic fields on a sintered cylindrical rod of YBa{sub 2}Cu{sub 3}O{sub 7} yield unambiguous evidence of independent inter- and intragranular contributions to the complex harmonic permeability {tilde {mu}}{sub n} = {mu}{prime}{sub n} -i{mu}{double prime}{sub n}. Temperature- dependence measurements reveal that, while the intragranular supercurrents disappear at {Tc}{ge}91.2 K, the intergranular supercurrents disappear at T{ge}86.6 K. This result is, to our knowledge, the first clear measurement of the phase-locking temperature of the 3-D matrix formed by YBa{sub 2}Cu{sub 3}O{sub 7} grains, which are in electrical contact with one another through weak links.