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Sample records for nf metropolitan

  1. Metropolitan Washington

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Metropolitan Washington Council of Governments A White House Climate Action Champions Case Study INDEX Executive Summary...............................2 Climate Action Champion.....................2 Project Spotlight.................................3-5 Co-benefits.............................................5 Challenges and Lessons Learned.........5 Resources and Contacts........................7 2 Executive Summary The Metropolitan Washington Council of Governments (COG) is an independent,

  2. Los Angeles County Metropolitan Transportation Authority Metro...

    Open Energy Info (EERE)

    County Metropolitan Transportation Authority Metro Jump to: navigation, search Name: Los Angeles County Metropolitan Transportation Authority (Metro) Place: Los Angeles, California...

  3. NF_Eder_2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    24 October 2013 Online at stacks.iop.orgNF53113037 Abstract Modelling and mitigation of damage are crucial for safe and economical operation of high-power laser facilities. ...

  4. Chicago Metropolitan Agency for Planning Data Dashboard

    Broader source: Energy.gov [DOE]

    The data dashboard for Chicago Metropolitan Agency for Planning, a partner in the Better Buildings Neighborhood Program.

  5. Metropolitan Edison Co (Pennsylvania) | Open Energy Information

    Open Energy Info (EERE)

    Edison Co (Pennsylvania) Jump to: navigation, search Name: Metropolitan Edison Co Place: Pennsylvania Phone Number: 1-800-545-7741 Website: www.firstenergycorp.comconten Twitter:...

  6. NF Energy Saving Corp | Open Energy Information

    Open Energy Info (EERE)

    NF Energy Saving Corp Jump to: navigation, search Name: NF Energy Saving Corp Place: Shenyang, Liaoning Province, China Sector: Services Product: China-based company provides...

  7. The Metropolitan Water District of Southern California

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SW Washington, DC 20585-0121 QERcomments@hq.doe.gov Comments on the Department of Energy's Quadrennial Energy Review: Water-Energy Nexus The Metropolitan Water District of Southern ...

  8. Metropolitan Water District of S CA | Open Energy Information

    Open Energy Info (EERE)

    Metropolitan Water District of S CA Jump to: navigation, search Name: Metropolitan Water District of S CA Place: California Phone Number: (213) 217-6000 Website: www.mwdh2o.com...

  9. Climate Action Champions: Metropolitan Washington Council of Governments,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DC, MD, and VA | Department of Energy Metropolitan Washington Council of Governments, DC, MD, and VA Climate Action Champions: Metropolitan Washington Council of Governments, DC, MD, and VA The Metropolitan Washington Council of Governments (COG) is an independent, nonprofit association that brings area leaders together to address major regional issues in the District of Columbia, suburban Maryland, and Northern Virginia. COG and its member governments seek to create a more accessible,

  10. Chicago Metropolitan Agency for Planning Summary of Reported Data |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Summary of Reported Data Chicago Metropolitan Agency for Planning Summary of Reported Data Summary of data reported by Better Buildings Neighborhood Program partner Chicago Metropolitan Agency for Planning. Chicago Metropolitan Agency for Planning Summary of Reported Data (762.93 KB) More Documents & Publications Better Buildings Neighborhood Program Summary of Reported Data From July 1, 2010 - September 30, 2013 Energize New York Summary of Reported Data Maryland

  11. Chicago Metropolitan Agency for Planning Data Dashboard | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    File Chicago Metropolitan Agency for Planning Data Dashboard More Documents & Publications Austin Energy Data Dashboard Massachusetts -- SEP Data Dashboard Phoenix, Arizona Data ...

  12. DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    60tifrancis2012o.pdf More Documents & Publications DeKalb CountyMetropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project DeKalb CountyMetropolitan...

  13. DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    vt060francis2010p.pdf More Documents & Publications DeKalb CountyMetropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project DeKalb CountyMetropolitan...

  14. DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    60tifrancis2011p.pdf More Documents & Publications DeKalb CountyMetropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project DeKalb CountyMetropolitan...

  15. ARM - VAP Product - aerich2nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Productsaerinfaerich2nf1turn Documentation Data Management Facility Plots (Quick Looks) Citation DOI: 10.54391027273 What is this? Generate Citation ARM Data Discovery Browse...

  16. ARM - VAP Product - aerich1nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Productsaerinfaerich1nf1turn Documentation Data Management Facility Plots (Quick Looks) Citation DOI: 10.54391027272 What is this? Generate Citation ARM Data Discovery Browse...

  17. Washington Metropolitan Area Transit Authority: Compressed Natural Gas Transit Bus Evaluation

    SciTech Connect (OSTI)

    Chandler, K.; Eberts, E.; Melendez, M.

    2006-04-01

    Evaluates compressed natural gas (CNG) powered transit buses at Washington Metropolitan Area Transit Authority (WMATA), providing a comparison between them and standard diesel transit buses.

  18. Backed apple? Metropolitan New York in the greenhouse

    SciTech Connect (OSTI)

    Hill, D.

    1997-12-31

    Steps to mitigate global climate change are being negotiated internationally, but it is on the local level that its effects will be felt and actions are taken. Like many midlatitude coastal cities, metropolitan New York could expect serious consequences from global warming: killing hot spells, worsened ozone pollution, uncertain water supply, and inundation of its waterfront from higher sea level and violent storms. Seen at the local level, the opportunities and limitations of measures to mitigate or adapt to climate change become explicit. Indirect local effects from changes elsewhere in the world must also be considered.

  19. Measuring and Mitigating Urban Warming in a Northern Metropolitan Area |

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Argonne Leadership Computing Facility Measuring and Mitigating Urban Warming in a Northern Metropolitan Area Event Sponsor: Environmental Science Seminar Start Date: Aug 25 2016 - 11:00am Building/Room: Building 240/Room 4301 Location: Argonne National Laboratory Speaker(s): Peter Snyder Speaker(s) Title: University of Minnesota In the United States and much of the rest of the world, cities are warming at twice the rate of outlying rural areas and the planet as a whole. While the warming can

  20. CMRR-NF Supplemental EIS Scoping Comments | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration | (NNSA) CMRR-NF Supplemental EIS Scoping Comments CMRR-NF Supplemental EIS Scoping Comments Additional CMRR Scoping Comments - 47A Additional CMRR Scoping Comments - 48A Additional CMRR Scoping Comments - 62 Additional CMRR Scoping Comments - 63 Additional CMRR Scoping Comments - 64 Additional CMRR Scoping Comments - 66A Additional CMRR Scoping Comments - 66B Additional CMRR Scoping Comments - 66C Additional CMRR Scoping Comments - 76 Additional CMRR Scoping Comments - 77

  1. Emission Testing of Washington Metropolitan Area Transit Authority (WMATA) Natural Gas and Diesel Transit Buses

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Emission Testing of Washington Metropolitan Area Transit Authority (WMATA) Natural Gas and Diesel Transit Buses M. Melendez, J. Taylor, and J. Zuboy National Renewable Energy Laboratory W.S. Wayne West Virginia University D. Smith U.S. Department of Energy Technical Report NREL/TP-540-36355 December 2005 Emission Testing of Washington Metropolitan Area Transit Authority (WMATA) Natural Gas and Diesel Transit Buses M. Melendez, J. Taylor, and J. Zuboy National Renewable Energy Laboratory W.S.

  2. Truck transport of RAM: Risk effects of avoiding metropolitan areas

    SciTech Connect (OSTI)

    Mills, G.S.; Neuhauser, K.S.

    1997-11-01

    In the transport of radioactive material (RAM), e.g., spent nuclear fuel (SNF), stakeholders are generally most concerned about risks in high population density areas along transportation routes because of the perceived high consequences of potential accidents. The most significant portions of a transcontinental route and an alternative examined previously were evaluated again using population density data derived from US Census Block data. This method of characterizing population that adjoins route segments offers improved resolution of population density variations, especially in high population density areas along typical transport routes. Calculated incident free doses and accident dose risks for these routes, and the rural, suburban and urban segments are presented for comparison of their relative magnitudes. The results indicate that modification of this route to avoid major metropolitan areas through use of non-Interstate highways increases total risk yet does not eliminate a relatively small urban component of the accident dose risk. This conclusion is not altered by improved resolution of route segments adjoining high density populations.

  3. IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section Knowledge on Oscillation Knowledge on Oscillation Measurements Measurements M. Sorel, IFIC (CSIC and U. of Valencia) IDS-NF, RAL, Jan 16-17 2008 M. Sorel - IFIC (Valencia U. & CSIC) 2 IDS-NF Neutrino Cross Sections: At What Energies Needed? Superbeams: Solid: T2K Dashed: NovA M. Sorel - IFIC (Valencia U. & CSIC) 3 IDS-NF Neutrino Cross Sections: At What Energies Needed? Superbeams: Solid: T2K Dashed: NovA Beta

  4. Smoothing single-crystalline SiC surfaces by reactive ion etching using pure NF{sub 3} and NF{sub 3}/Ar mixture gas plasmas

    SciTech Connect (OSTI)

    Tasaka, Akimasa; Kotaka, Yuki; Oda, Atsushi; Saito, Morihiro; Tojo, Tetsuro; Inaba, Minoru

    2014-09-01

    In pure NF{sub 3} plasma, the etching rates of four kinds of single-crystalline SiC wafer etched at NF{sub 3} pressure of 2 Pa were the highest and it decreased with an increase in NF{sub 3} pressure. On the other hand, they increased with an increase in radio frequency (RF) power and were the highest at RF power of 200 W. A smooth surface was obtained on the single-crystalline 4H-SiC after reactive ion etching at NF{sub 3}/Ar gas pressure of 2 Pa and addition of Ar to NF{sub 3} plasma increased the smoothness of SiC surface. Scanning electron microscopy observation revealed that the number of pillars decreased with an increase in the Ar-concentration in the NF{sub 3}/Ar mixture gas. The roughness factor (R{sub a}) values were decreased from 51.5 nm to 25.5 nm for the As-cut SiC, from 0.25 nm to 0.20 nm for the Epi-SiC, from 5.0 nm to 0.7 nm for the Si-face mirror-polished SiC, and from 0.20 nm to 0.16 nm for the C-face mirror-polished SiC by adding 60% Ar to the NF{sub 3} gas. Both the R{sub a} values of the Epi- and the C-face mirror-polished wafer surfaces etched using the NF{sub 3}/Ar (40:60) plasma were similar to that treated with mirror polishing, so-called the Catalyst-Referred Etching (CARE) method, with which the lowest roughness of surface was obtained among the chemical mirror polishing methods. Etching duration for smoothing the single-crystalline SiC surface using its treatment was one third of that with the CARE method.

  5. Gasoline distribution cycle and vapor emissions in Mexico City metropolitan area

    SciTech Connect (OSTI)

    Molina, M.M.; Secora, I.S.; Gallegos, J.R.M.; Grapain, V.M.G.; Villegas, F.M.R.; Flores, L.A.M.

    1997-12-31

    Ozone in the main air pollutant in Mexico City Metropolitan Area (MCMA). This kind of pollution is induced by the emissions of nitrogen oxides and hydrocarbons. According to Official Statistics National Air Pollution Quality Standard is exceeded over 300 days a year. Volatile hydrocarbons are generated in the cycle of storage transport and distribution of fuel (Gasoline Distribution Cycle). Above 17 millions of liters are handled daily in MCMA. Evaporative emission control is a complex task involving: floating roof tanks and vapor recovery units installation at bulk terminals and implementation of Phase 1 and Phase 2 vapor recovery systems at service stations. Since 1990, IMP has been involved in researching vapor emissions associated to gasoline storage and distribution cycle. Besides, the authors evaluate several technologies for bulk terminals and service stations. In this job, the authors present the results of an evaluation according to Mexican Official Standard of 500 vehicles. The gasoline vapors are trapped during refueling of cars and they are conduced to an equipment that includes an activated charcoal canister in order to adsorb them. Another Activated charcoal canister adsorbs ambient air as a reference. Experimental results showed that refueling hydrocarbon emissions are between 0.4 and 1.2 grams per liter with averages of 0.79 and 0.88 grams per liter according with two different gasoline types. These results were applied to Mexico City Vehicular fleet for the gasoline distribution cycle in order to obtain a total volatile hydrocarbon emission in Mexico City Metropolitan Area.

  6. Metropolitan Washington

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... local communities and agencies to surpass this goal. Bulk renewable energy ... Procurement Program operates with the ... County and the Cities of Bowie and ...

  7. Smad7 mediates inhibition of Saos2 osteosarcoma cell differentiation by NF{kappa}B

    SciTech Connect (OSTI)

    Eliseev, Roman A. . E-mail: Roman_Eliseev@urmc.rochester.edu; Schwarz, Edward M.; Zuscik, Michael J.; O'Keefe, Regis J.; Drissi, Hicham; Rosier, Randy N.

    2006-01-01

    The transcription factor NF{kappa}B is constitutively activated in various tumor cells where it promotes proliferation and represses apoptosis. The bone morphogenetic proteins (BMPs) delay cell proliferation and promote differentiation and apoptosis of bone cells through activation of Smad downstream effectors and via Smad-independent mechanisms. Thus, NF{kappa}B and BMP pathways play opposing roles in regulating osteoblastic cell fate. Here, we show that in osteosarcoma Saos2 osteoblasts, NF{kappa}B regulates the activity of the BMP/Smad signaling. Inhibition of NF{kappa}B by overexpression of mI{kappa}B leads to the induction of osteoblast differentiation. Saos2 cells overexpressing mI{kappa}B (Saos2-mI{kappa}B) exhibit higher expression of osteoblast phenotypic genes such as alkaline phosphatase, Runx2 and osteocalcin and are more responsive to BMP2 in comparison to wild-type cells (Saos2-wt) or empty vector infected controls (Saos2-EV). Furthermore, BMP-2 signaling and Smad phosphorylation are significantly increased in Saos2-mI{kappa}B cells in comparison to Saos2-EV cells. Inhibition of NF{kappa}B signaling in Saos2-mI{kappa}B cells is associated with decreased expression of the BMP signaling inhibitor Smad7. While gain of Smad7 function in Saos2-mI{kappa}B cells results in inhibition of BMP signaling, anti-sense knockdown of Smad7 in Saos2-EV cells leads to upregulation of BMP signaling. We therefore conclude that in osteosarcoma Saos2 cells, NF{kappa}B represses BMP/Smad signaling and BMP2-induced differentiation through Smad7.

  8. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  9. Withaferin A disrupts ubiquitin-based NEMO reorganization induced by canonical NF-κB signaling

    SciTech Connect (OSTI)

    Jackson, Shawn S.; Oberley, Christopher; Hooper, Christopher P.; Grindle, Kreg; Wuerzberger-Davis, Shelly; Wolff, Jared; and others

    2015-02-01

    The NF-κB family of transcription factors regulates numerous cellular processes, including cell proliferation and survival responses. The constitutive activation of NF-κB has also emerged as an important oncogenic driver in many malignancies, such as activated B-cell like diffuse large B cell lymphoma, among others. In this study, we investigated the impact and mechanisms of action of Withaferin A, a naturally produced steroidal lactone, against both signal-inducible as well as constitutive NF-κB activities. We found that Withaferin A is a robust inhibitor of canonical and constitutive NF-κB activities, leading to apoptosis of certain lymphoma lines. In the canonical pathway induced by TNF, Withaferin A did not disrupt RIP1 polyubiquitination or NEMO–IKKβ interaction and was a poor direct IKKβ inhibitor, but prevented the formation of TNF-induced NEMO foci which colocalized with TNF ligand. While GFP-NEMO efficiently formed TNF-induced foci, a GFP-NEMO{sup Y308S} mutant that is defective in binding to polyubiquitin chains did not form foci. Our study reveals that Withaferin A is a novel type of IKK inhibitor which acts by disrupting NEMO reorganization into ubiquitin-based signaling structures in vivo. - Highlights: • Withaferin A, a NF-κB inhibitor, disrupts signaling induced NEMO localization, a novel point of inhibition. • NEMO can be localized to distinct signaling foci after treatment with TNF. • ABC-type DLCBL cells can be sensitized to apoptosis after treatment with Withaferin A.

  10. Piperlongumine selectively suppresses ABC-DLBCL through inhibition of NF-κB p65 subunit nuclear import

    SciTech Connect (OSTI)

    Niu, Mingshan; Shen, Yangling; Xu, Xiaoyu; Yao, Yao; Fu, Chunling; Yan, Zhiling; Wu, Qingyun; Cao, Jiang; Sang, Wei; Zeng, Lingyu; Li, Zhenyu; Liu, Xuejiao; and others

    2015-07-10

    Constitutive NF-κB activation is required for survival of activated B cell-like subtype of diffuse large B cell lymphoma (ABC-DLBCL). However, current NF-κB targeting strategies lack cancer cell specificity. Here, we identified a novel inhibitor, piperlongumine, features direct binding to NF-κB p65 subunit and suppression of p65 nuclear import. This was accompanied by NF-κB reporter activity suppression and NF-κB target gene downregulation. Moreover, mutation of Cys{sup 38} to Ser in p65 abolished this effect of piperlongumine on inhibition of p65 nuclear import. Furthermore, we show that piperlongumine selectively inhibited proliferation and induced apoptosis of ABC-DLBCL cells. Most notably, it has been reported that piperlongumine did not affect normal cells even at high doses and was nontoxic to animals. Hence, our current study provides new insight into piperlongumine's mechanism of action and novel approach to ABC-DLBCL target therapy. - Highlights: • Current NF-κB targeting strategies lack cancer cell specificity. • Piperlongumine inhibits NF-κB p65 subunit nuclear import via directly binding to p65. • Piperlongumine selectively inhibits proliferation of ABC-DLBCL cells. • This study provides a novel approach to ABC-DLBCL target therapy.

  11. Coal dust contiguity-induced changes in the concentration of TNF- and NF- B p65 on the ocular surface

    SciTech Connect (OSTI)

    Sun, Z.Y.; Hong, J.; Liu, Z.Y.; Jin, X.D.; Gu, C.H.

    2009-07-01

    To observe the influence of coal dust on ocular surface of coal miners and rabbits with coal dust contiguity on expression TNF- and NF- Bp65 and dry eye occurrence. Expression TNF- and NF- Bp65 in ocular surface were determined. Results showed tear production, BUT and lysozyme decreased for coal miners and rabbits with coal dust contiguity. Coal dust exposure was linked to development of xerophthalmia, and induced a higher expression of NF- B p65 and TNF- perhaps as a mechanism to resist coal dust ocular surface injury.

  12. The influence of coyotes on an urban Canada goose population in the Chicago metropolitan area

    SciTech Connect (OSTI)

    Brown, Justin L.; /Ohio State U.

    2007-01-01

    Canada geese (Branta canadensis) have become common in many urban areas, often creating nuisance problems for human residents. The presence of urban geese has raised concerns about the spread of disease, increased erosion, excessive noise, eutrophication of waterways, and general nuisance problems. Goose populations have grown due to an increase in urbanization resulting in an abundance of high quality food (urban grass) and suitable nesting sites, as well as a decrease in some predators. I monitored nest predation in the Chicago suburbs during the 2004 and 2005 nesting seasons using 3 nest monitoring techniques to identify predators: video cameras, plasticine eggs, and sign from nest using a classification tree analysis. Of 58 nests monitored in 2004 and 286 in 2005, only raccoons (Procyon lotor) and coyotes (Canis latrans) were identified as nest predators. Raccoons were responsible for 22-25% of depredated nests, but were rarely capable of depredating nests that were actively defended by a goose. Coyotes were responsible for 75-78% of all Canada goose nest depredation and were documented killing one adult goose and feeding on several others. The coyote is a top-level predator that had increased in many metropolitan areas in recent years. To determine if coyotes were actively hunting geese or eggs during the nesting season, I analyzed coyote habitat selection between nesting and pre-nesting or post-nesting seasons. Coyote home ranges (95% Minimum Convex Polygon) were calculated for 19 coyotes to examine third order habitat selection related to goose nest abundance. A 100 m buffer (buffer habitat) was created and centered on each waterway edge and contained 90% of all nests. Coyotes showed selection for habitats during all seasons. Buffer habitat was the top ranked habitat in both pre-nesting and nesting seasons, but dropped to third ranked in post-nesting season. Habitat selection across seasons was compared using a repeated measures MANOVA. Habitat selection

  13. BRAF activated non-coding RNA (BANCR) promoting gastric cancer cells proliferation via regulation of NF-κB1

    SciTech Connect (OSTI)

    Zhang, Zhi-Xin; Liu, Zhi-Qiang; Jiang, Biao; Lu, Xin-Yang; Ning, Xiao-Fei; Yuan, Chuan-Tao; Wang, Ai-Liang

    2015-09-18

    Background and objective: Long non-coding RNA, BANCR, has been demonstrated to contribute to the proliferation and migration of tumors. However, its molecular mechanism underlying gastric cancer is still unknown. In present study, we investigated whether BANCR was involved in the development of gastric cancer cells via regulation of NF-κB1. Methods: Human gastric cancer tissues were isolated as well as human gastric cell lines MGC803 and BGC823 were cultured to investigate the role of BANCR in gastric cancer. Results: BANCR expression was significantly up-regulated in gastric tumor tissues and gastric cell lines. Down-regulation of BANCR inhibited gastric cancer cell growth and promoted cell apoptosis, and it also contributed to a significant decrease of NF-κB1 (P50/105) expression and 3′UTR of NF-κB1 activity. Overexpression of NF-κB1 reversed the effect of BANCR on cancer cell growth and apoptosis. MiroRNA-9 (miR-9) targeted NF-κB1, and miR-9 inhibitor also reversed the effects of BANCR on gastric cancer cell growth and apoptosis. Conclusion: BANCR was highly expressed both in gastric tumor tissues and in cancer cells. NF-κB1 and miR-9 were involved in the role of BANCR in gastric cancer cell growth and apoptosis. - Highlights: • BANCR up-regulated in gastric cancer (GC) tissues and cell lines MGC803 and BGC823. • Down-regulation of BANCR inhibited GC cell growth and promoted cell apoptosis. • Down-regulation of BANCR contributed to decreased 3′UTR of NF-κB1 and its expression. • Overexpressed NF-κB1 reversed the effect of BANCR on GC cell growth. • miR-9 inhibitor reversed the effect of BANCR on cancer GC cell growth.

  14. XEDAR activates the non-canonical NF-κB pathway

    SciTech Connect (OSTI)

    Verhelst, Kelly; Gardam, Sandra; Borghi, Alice; Kreike, Marja; Carpentier, Isabelle; Beyaert, Rudi

    2015-09-18

    Members of the tumor necrosis factor receptor (TNFR) superfamily are involved in a number of physiological and pathological responses by activating a wide variety of intracellular signaling pathways. The X-linked ectodermal dysplasia receptor (XEDAR; also known as EDA2R or TNFRSF27) is a member of the TNFR superfamily that is highly expressed in ectodermal derivatives during embryonic development and binds to ectodysplasin-A2 (EDA-A2), a member of the TNF family that is encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Although XEDAR was first described in the year 2000, its function and molecular mechanism of action is still largely unclear. XEDAR has been reported to activate canonical nuclear factor κB (NF-κB) signaling and mitogen-activated protein (MAP) kinases. Here we report that XEDAR is also able to trigger the non-canonical NF-κB pathway, characterized by the processing of p100 (NF-κB2) into p52, followed by nuclear translocation of p52 and RelB. We provide evidence that XEDAR-induced p100 processing relies on the binding of XEDAR to TRAF3 and TRAF6, and requires the kinase activity of NIK and IKKα. We also show that XEDAR stimulation results in NIK accumulation and that p100 processing is negatively regulated by TRAF3, cIAP1 and A20. - Highlights: • XEDAR activates the non-canonical NF-κB pathway. • XEDAR-induced processing of p100 depends on XEDAR interaction with TRAF3 and TRAF6. • XEDAR-induced processing of p100 depends on NIK and IKKα activity. • Overexpression of XEDAR leads to NIK accumulation. • XEDAR-induced processing of p100 is negatively regulated by TRAF3 cIAP1 and A20.

  15. Agreement Execution Process Study: CRADAs and NF-WFO Agreements and the Speed of Business

    SciTech Connect (OSTI)

    Harrer, Bruce J.; Cejka, Cheryl L.; Macklin, Richard; Miksovic, Ann

    2011-02-01

    This report summarizes the findings of a study on the execution of Cooperative Research and Development Agreements (CRADAs) and Non-Federal Work for Others (NF-WFO) agreements across the U.S. Department of Energy (DOE) laboratory complex. The study provides quantitiative estimates of times required to negotiate and execute these agreements across the DOE complex. It identifies factors impacting on cycle times and describes best practicies used at various laboratories and site offices that reduce cycle times.

  16. Chemical evolution of volatile organic compounds in the outflow of the Mexico City Metropolitan area

    SciTech Connect (OSTI)

    Apel, Eric; Emmons, L.; Karl, Thomas G.; Flocke, Frank M.; Hills, A. J.; Madronich, Sasha; Lee-Taylor, J.; Fried, Alan; Weibring, P.; Walega, J.; Richter, Dirk; Tie, X.; Mauldin, L.; Campos, Teresa; Weinheimer, Andrew J.; Knapp, David; Sive, B.; Kleinman, Lawrence I.; Springston, S.; Zaveri, Rahul A.; Ortega, John V.; Voss, Paul B.; Blake, D. R.; Baker, Angela K.; Warneke, Carsten; Welsh-Bon, Daniel; de Gouw, Joost A.; Zheng, J.; Zhang, Renyi; Rudolph, Jochen; Junkermann, W.; Riemer, D.

    2010-01-01

    The volatile organic compound (VOC) distribution in the Mexico City Metropolitan Area (MCMA) and its evolution as it is uplifted and transported out of the MCMA basin was studied during the 2006 MILAGRO/MIRAGE-Mex field campaign. The results show that in the morning hours in the city center, the VOC distribution is dominated by non-methane hydrocarbons (NMHCs) but with a substantial contribution from oxygenated volatile organic compounds (OVOCs), predominantly from primary emissions. Alkanes account for a large part of the NMHC distribution in terms of mixing ratios. In terms of reactivity, NMHCs also dominate overall, especially in the morning hours. However, in the afternoon, as the boundary layer lifts and air is mixed and aged within the basin, the distribution changes as secondary products are formed. The WRF-Chem (Weather Research and Forecasting with Chemistry) model and MOZART (Model for Ozone and Related chemical Tracers) were able to reproduce the general features of the daytime cycle of the VOC OH reactivity distribution showing that NMHCs dominate the distribution except in the afternoon hours and that the VOC OH reactivity peaks in the early morning due to high morning emissions from the city into a shallow boundary layer. The WRF-Chem and MOZART models showed higher reactivity than the experimental data during the nighttime cycle, perhaps indicating problems with the modeled nighttime boundary layer height. In addition, a plume was studied in which air was advected out of the MCMA and intercepted downwind with the DOE G1 on March 18 and the NCAR C130 one day later on March 19. A number of identical species measured aboard each aircraft gave insight into the chemical evolution of the plume as it aged and was transported as far as 1000 km downwind. Ozone and many OVOCs were photochemically produced in the plume. The WRF-Chem and MOZART models were used to examine the spatial and temporal extent of the March 19 plume and to help interpret the OH

  17. Chemical evolution of volatile organic compounds in the outflow of the Mexico City Metropolitan area

    SciTech Connect (OSTI)

    Apel, E.; Springston, S.; Karl, T.; Emmons, L.; Flocke, F.; Hills, A. J.; Madronich, S.; Lee-Taylor, J.; Fried, A.; Weibring, P.; Walega, J.; Richter, D., Tie, X.; Mauldin, L.; Campos, T.; Sive, B.; Kleinman, L.; Springston, S., Zaveri, R.; deGouw, J.; Zheng, J.; Zhang, R.; Rudolph, J.; Junkermann, W.; Riemer, D. D.

    2009-11-01

    The volatile organic compound (VOC) distribution in the Mexico City Metropolitan Area (MCMA) and its evolution as it is uplifted and transported out of the MCMA basin was studied during the 2006 MILAGRO/MIRAGE-Mex field campaign. The results show that in the morning hours in the city center, the VOC distribution is dominated by non-methane hydrocarbons (NMHCs) but with a substantial contribution from oxygenated volatile organic compounds (OVOCs), predominantly from primary emissions. Alkanes account for a large part of the NMHC distribution in terms of mixing ratios. In terms of reactivity, NMHCs also dominate overall, especially in the morning hours. However, in the afternoon, as the boundary layer lifts and air is mixed and aged within the basin, the distribution changes as secondary products are formed. The WRF-Chem (Weather Research and Forecasting with Chemistry) model and MOZART (Model for Ozone and Related chemical Tracers) were able to reproduce the general features of the daytime cycle of the VOC OH reactivity distribution showing that NMHCs dominate the distribution except in the afternoon hours and that the VOC OH reactivity peaks in the early morning due to high morning emissions from the city into a shallow boundary layer. The WRF-Chem and MOZART models showed higher reactivity than the experimental data during the nighttime cycle, perhaps indicating problems with the modeled nighttime boundary layer height. In addition, a plume was studied in which air was advected out of the MCMA and intercepted downwind with the DOE G1 on 18 March and the NCAR C130 one day later on 19 March. A number of identical species measured aboard each aircraft gave insight into the chemical evolution of the plume as it aged and was transported as far as 1000 km downwind. Ozone and many OVOCs were photochemically produced in the plume. The WRF-Chem and MOZART models were used to examine the spatial and temporal extent of the 19 March plume and to help interpret the OH

  18. Characterization of the Kinetics of NF3-Fluorination of NpO2

    SciTech Connect (OSTI)

    Casella, Andrew M.; Scheele, Randall D.; McNamara, Bruce K.

    2015-12-23

    The exploitation of selected actinide and fission product fluoride volatilities has long been considered as a potentially attractive compact method for recycling used nuclear fuels to avoid generating the large volumes of radioactive waste arising from aqueous reprocessing [1-7]. The most developed process uses the aggressive and hazardous fluorinating agents hydrogen fluoride (HF) and/or molecular fluorine (F2) at high temperatures to volatilize the greatest fraction of the used nuclear fuel into a single gas stream. The volatilized fluorides are subsequently separated using a series of fractionation and condensation columns to recover the valuable fuel constituents and fission products. In pursuit of a safer and less complicated approach, we investigated an alternative fluoride volatility-based process using the less hazardous fluorinating agent nitrogen trifluoride (NF3) and leveraging its less aggressive nature to selectively evolve fission product and actinide fluorides from the solid phase based on their reaction temperatures into a single recycle stream [8-15]. In this approach, successive isothermal treatments using NF3 will first evolve the more thermally susceptible used nuclear fuel constituents leaving the other constituents in the residual solids until subsequent isothermal temperature treatments cause these others to volatilize. During investigation of this process, individual neat used fuel components were treated with isothermal NF3 in an attempt to characterize the kinetics of each fluorination reaction to provide input into the design of a new volatile fluoride separations approach. In these directed investigations, complex behavior was observed between NF3 and certain solid reactants such as the actinide oxides of uranium, plutonium, and neptunium. Given the similar thermal reaction susceptibilities of neptunium oxide (NpO2) and uranium dioxide (UO2) and the importance of Np and U, we initially focused our efforts on determining the reaction

  19. Environmental benefits of replacing fuel oil by natural gas in the metropolitan region of Sao Paulo, Brazil

    SciTech Connect (OSTI)

    Kondo, S.; Assuncao, J.V. de

    1998-12-31

    The Metropolitan Region of Sao Paulo (Brazil) has a population 16.322 million people (1995 estimate) living in an area of 8,051 km2 with most of them concentrated in the city of Sao Paulo with 9.8 million people and 4.6 million cars. Although with an air quality better than some other Latin American megacities such as Mexico and Santiago do Chile, the air quality still exceeds the national air quality standards. In 2/17/1993 Brazilian Petroleum Company (PETROBRAS) and the Bolivian Petroleum Company (Yacimientos Petroliferos Fiscales Bolivianos -- YPFB) signed an agreement to bring natural gas from Bolivia to the south and southeast of Brazil. The end of the construction of the gas pipeline will be in 1999, and it will deliver 4 million Nm3/day of natural gas to COMGAS Sao Paulo State Gas Company. This amount will increase to 8.1 million Nm3/day by the year 2006, that will be sufficient to supply the Sao Paulo Metropolitan Region market need at that time. In this study an estimate of the influence in the air quality was performed supposing the substitution of fuel oil by natural gas in industry and also in diesel buses. The results showed that there will be benefits in relation to sulfur dioxide, PM10, greenhouse gases and trace elements, and negligible effects in relation to NO{sub x}, NMTOC and carbon monoxide.

  20. Nickel differentially regulates NFAT and NF-{kappa}B activation in T cell signaling

    SciTech Connect (OSTI)

    Saito, Rumiko; Hirakawa, Satoshi; Ohara, Hiroshi; Yasuda, Makoto; Yamazaki, Tomomi; Nishii, Shigeaki; Aiba, Setsuya

    2011-08-01

    Nickel is a potent hapten that induces contact hypersensitivity in human skin. While nickel induces the maturation of dendritic cells via NF-{kappa}B and p38 MAPK activation, it also exerts immunosuppressive effects on T cells through an unknown mechanism. To elucidate the molecular mechanisms of its effects on T cells, we examined the effects of NiCl{sub 2} on mRNA expression in human CD3+ T cells stimulated with CD3 and CD28 antibodies. Using a DNA microarray and Gene Ontology, we identified 70 up-regulated (including IL-1{beta}, IL-6 and IL-8) and 61 down-regulated (including IL-2, IL-4, IL-10 and IFN-{gamma}) immune responsive genes in NiCl{sub 2}-treated T cells. The DNA microarray results were verified using real-time PCR and a Bio-Plex{sup TM} suspension protein array. Suppression of IL-2 and IFN-{gamma} gene transcription by NiCl{sub 2} was also confirmed using Jurkat T cells transfected with IL-2 or IFN-{gamma} luciferase reporter genes. To explore the NiCl{sub 2}-regulated signaling pathway, we examined the binding activity of nuclear proteins to NFAT, AP-1, and NF-{kappa}B consensus sequences. NiCl{sub 2} significantly and dose-dependently suppressed NFAT- and AP-1-binding activity, but augmented NF-{kappa}B-binding activity. Moreover, NiCl{sub 2} decreased nuclear NFAT expression in stimulated T cells. Using Jurkat T cells stimulated with PMA/ionomycin, we demonstrated that NiCl{sub 2} significantly suppressed stimulation-evoked cytosolic Ca{sup 2+} increases, suggesting that NiCl{sub 2} regulates NFAT signals by acting as a blocker of Ca{sup 2+} release-activated Ca{sup 2+} (CRAC) channels. These data showed that NiCl{sub 2} decreases NFAT and increases NF-{kappa}B signaling in T cells. These results shed light on the effects of nickel on the molecular regulation of T cell signaling. - Graphical Abstract: Nickel suppresses stimulation-evoked cytosolic Ca{sup 2+} increase, which results in the suppression of NFAT signals. On the other hand, Ni rather

  1. Constitutive NF-?B activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production

    SciTech Connect (OSTI)

    Chung, Jin Sil; Lee, Sora; Yoo, Young Do

    2014-08-08

    Highlights: Romo1 expression is required for constitutive nuclear DNA-binding activity of NF-?B. Romo1 depletion suppresses tumor growth in vivo. Romo1 presents a potential therapeutic target for diseases. - Abstract: Deregulation of nuclear factor-?B (NF-?B) and related pathways contribute to tumor cell proliferation and invasion. Mechanisms for constitutive NF-?B activation are not fully explained; however, the underlying defects appear to generate and maintain pro-oxidative conditions. In hepatocellular carcinoma (HCC) tissues, up-regulation of reactive oxygen species modulator 1 (Romo1) correlates positively with tumor size. In the present study, we showed that Romo1 expression is required to maintain constitutive nuclear DNA-binding activity of NF-?B and transcriptional activity through constitutive I?B? phosphorylation. Overexpression of Romo1 promoted p65 nuclear translocation and DNA-binding activity. We also show that Romo1 depletion suppressed anchorage-independent colony formation by HCC cells and suppressed tumor growth in vivo. Based on these findings, Romo1 may be a principal regulatory factor in the maintenance of constitutive NF-?B activation in tumor cells. In the interest of anti-proliferative treatments for cancer, Romo1 may also present a productive target for drug development.

  2. XIAP Induces NF-kB Activation via the BIR1/TAB1 Interaction and BIR1 Dimerization

    SciTech Connect (OSTI)

    Lu,M.; Lin, S.; Huang, Y.; Kang, Y.; Rich, R.; Lo, Y.; Myszka, D.; Han, J.; Wu, H.

    2007-01-01

    In addition to caspase inhibition, X-linked inhibitor of apoptosis (XIAP) induces NF-{kappa}B and MAP kinase activation during TGF-b and BMP receptor signaling and upon overexpression. Here we show that the BIR1 domain of XIAP, which has no previously ascribed function, directly interacts with TAB1 to induce NF-{kappa}B activation. TAB1 is an upstream adaptor for the activation of the kinase TAK1, which in turn couples to the NF-{kappa}B pathway. We report the crystal structures of BIR1, TAB1, and the BIR1/TAB1 complex. The BIR1/TAB1 structure reveals a striking butterfly-shaped dimer and the detailed interaction between BIR1 and TAB1. Structure-based mutagenesis and knockdown of TAB1 show unambiguously that the BIR1/TAB1 interaction is crucial for XIAP-induced TAK1 and NF-{kappa}B activation. We show that although not interacting with BIR1, Smac, the antagonist for caspase inhibition by XIAP, also inhibits the XIAP/TAB1 interaction. Disruption of BIR1 dimerization abolishes XIAP-mediated NF-{kappa}B activation, implicating a proximity-induced mechanism for TAK1 activation.

  3. Mathematical Modeling of Hepatitis C Prevalence Reduction with Antiviral Treatment Scale-Up in Persons Who Inject Drugs in Metropolitan Chicago

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Echevarria, Desarae; Gutfraind, Alexander; Boodram, Basmattee; Major, Marian; Del Valle, Sara; Cotler, Scott J.; Dahari, Harel

    2015-08-21

    New direct-acting antivirals (DAAs) provide an opportunity to combat hepatitis C virus (HCV) infection in persons who inject drugs (PWID). Here we use a mathematical model to predict the impact of a DAA-treatment scale-up on HCV prevalence among PWID and the estimated cost in metropolitan Chicago.

  4. FY13 Summary Report on the Augmentation of the Spent Fuel Composition Dataset for Nuclear Forensics: SFCOMPO/NF

    SciTech Connect (OSTI)

    Brady Raap, Michaele C.; Lyons, Jennifer A.; Collins, Brian A.; Livingston, James V.

    2014-03-31

    This report documents the FY13 efforts to enhance a dataset of spent nuclear fuel isotopic composition data for use in developing intrinsic signatures for nuclear forensics. A review and collection of data from the open literature was performed in FY10. In FY11, the Spent Fuel COMPOsition (SFCOMPO) excel-based dataset for nuclear forensics (NF), SFCOMPO/NF was established and measured data for graphite production reactors, Boiling Water Reactors (BWRs) and Pressurized Water Reactors (PWRs) were added to the dataset and expanded to include a consistent set of data simulated by calculations. A test was performed to determine whether the SFCOMPO/NF dataset will be useful for the analysis and identification of reactor types from isotopic ratios observed in interdicted samples.

  5. Comparison of Daytime and Nighttime Populations Adjacent to Interstate Highways in Metropolitan Areas Using LandScan USA

    SciTech Connect (OSTI)

    Johnson, Paul E

    2007-01-01

    An article of similar title was published in the International Journal of Radioactive Materials Transport in 1999. The study concluded that the daytime and nighttime populations are not substantially different for the metropolitan areas examined. This study revisits the issue, but using the LandScan USA high resolution population distribution data, which includes daytime and night-time population. Segments of Interstate highway beltways, along with the direct route through the city, for Atlanta, St. Louis, and Kansas City are examined with an 800m buffer from either side of the highways. The day/night ratio of population is higher using the LandScan USA data. LandScan USA daytime and night-time data will be incorporated into the TRAGIS routing model in future.

  6. Intranuclear interactomic inhibition of NF-κB suppresses LPS-induced severe sepsis

    SciTech Connect (OSTI)

    Park, Sung-Dong; Cheon, So Yeong; Park, Tae-Yoon; Shin, Bo-Young; Oh, Hyunju; Ghosh, Sankar; Koo, Bon-Nyeo; Lee, Sang-Kyou

    2015-08-28

    Suppression of nuclear factor-κB (NF-κB) activation, which is best known as a major regulator of innate and adaptive immune responses, is a potent strategy for the treatment of endotoxic sepsis. To inhibit NF-κB functions, we designed the intra-nuclear transducible form of transcription modulation domain (TMD) of RelA (p65), called nt-p65-TMD, which can be delivered effectively into the nucleus without influencing the cell viability, and work as interactomic inhibitors via disruption of the endogenous p65-mediated transcription complex. nt-p65-TMD effectively inhibited the secretion of pro-inflammatory cytokines, including TNF-α, IL-1β, or IL-6 from BV2 microglia cells stimulated by lipopolysaccharide (LPS). nt-p65-TMD did not inhibit tyrosine phosphorylation of signaling mediators such as ZAP-70, p38, JNK, or ERK involved in T cell activation, but was capable of suppressing the transcriptional activity of NF-κB without the functional effect on that of NFAT upon T-cell receptor (TCR) stimulation. The transduced nt-p65-TMD in T cell did not affect the expression of CD69, however significantly inhibited the secretion of T cell-specific cytokines such as IL-2, IFN-γ, IL-4, IL-17A, or IL-10. Systemic administration of nt-p65-TMD showed a significant therapeutic effect on LPS-induced sepsis model by inhibiting pro-inflammatory cytokines secretion. Therefore, nt-p65-TMD can be a novel therapeutics for the treatment of various inflammatory diseases, including sepsis, where a transcription factor has a key role in pathogenesis, and further allows us to discover new functions of p65 under normal physiological condition without genetic alteration. - Highlights: • The nt-p65-TMD is intra-nuclear interactomic inhibitor of endogenous p65. • The nt-p65-TMD effectively inhibited the secretion of pro-inflammatory cytokines. • The excellent therapeutic potential of nt-p65-TMD was confirmed in sepsis model.

  7. 3D calculation of Tucson-Melbourne 3NF effect in triton binding energy

    SciTech Connect (OSTI)

    Hadizadeh, M. R.; Tomio, L.; Bayegan, S.

    2010-08-04

    As an application of the new realistic three-dimensional (3D) formalism reported recently for three-nucleon (3N) bound states, an attempt is made to study the effect of three-nucleon forces (3NFs) in triton binding energy in a non partial wave (PW) approach. The spin-isospin dependent 3N Faddeev integral equations with the inclusion of 3NFs, which are formulated as function of vector Jacobi momenta, specifically the magnitudes of the momenta and the angle between them, are solved with Bonn-B and Tucson-Melbourne NN and 3N forces in operator forms which can be incorporated in our 3D formalism. The comparison with numerical results in both, novel 3D and standard PW schemes, shows that non PW calculations avoid the very involved angular momentum algebra occurring for the permutations and transformations and it is more efficient and less cumbersome for considering the 3NF.

  8. Interleukin 6 promotes endometrial cancer growth through an autocrine feedback loop involving ERKNF-?B signaling pathway

    SciTech Connect (OSTI)

    Che, Qi; Liu, Bin-Ya; Wang, Fang-Yuan; He, Yin-Yan; Lu, Wen; Liao, Yun; Gu, Wei; Wan, Xiao-Ping

    2014-03-28

    Highlights: IL-6 could promote endometrial cancer cells proliferation. IL-6 promotes its own production through an autocrine feedback loop. ERK and NF-?B pathway inhibitors inhibit IL-6 production and tumor growth. IL-6 secretion relies on the activation of ERKNF-?B pathway axis. An orthotopic nude endometrial carcinoma model confirms the effect of IL-6. - Abstract: Interleukin (IL)-6 as an inflammation factor, has been proved to promote cancer proliferation in several human cancers. However, its role in endometrial cancer has not been studied clearly. Previously, we demonstrated that IL-6 promoted endometrial cancer progression through local estrogen biosynthesis. In this study, we proved that IL-6 could directly stimulate endometrial cancer cells proliferation and an autocrine feedback loop increased its production even after the withdrawal of IL-6 from the medium. Next, we analyzed the mechanism underlying IL-6 production in the feedback loop and found that its production and IL-6-stimulated cell proliferation were effectively blocked by pharmacologic inhibitors of nuclear factor-kappa B (NF-?B) and extra-cellular signal-regulated kinase (ERK). Importantly, activation of ERK was upstream of the NF-?B pathways, revealing the hierarchy of this event. Finally, we used an orthotopic nude endometrial carcinoma model to confirm the effects of IL-6 on the tumor progression. Taken together, these data indicate that IL-6 promotes endometrial carcinoma growth through an expanded autocrine regulatory loop and implicate the ERKNF-?B pathway as a critical mediator of IL-6 production, implying IL-6 to be an important therapeutic target in endometrial carcinoma.

  9. 27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

    SciTech Connect (OSTI)

    Kim, Sun-Mi; Lee, Chung Won; Kim, Bo-Young; Jung, Young-Suk; Eo, Seong-Kug; Park, Young Chul; Kim, Koanhoi

    2015-08-07

    We attempted to determine the effects of a milieu rich in cholesterol molecules on expression of chemokine CXCL8. A high-cholesterol diet led to an increased transcription of the IL-8 gene in the arteries and elevated levels of CXCL8 in sera of ApoE{sup −/−} mice, compared with those of wild-type C57BL/6 mice. Treatment of THP-1 monocyte/macrophage cells with 27-hydroxycholesterol (27OHChol) resulted in transcription of the IL-8 gene and increased secretion of its corresponding gene product whereas cholesterol did not induce expression of CXCL8 in THP-1 cells. 27OHChol-induced transcription of the IL-8 gene was blocked by cycloheximide, but not by polymyxin B. Treatment of THP-1 cells with 27OHChol caused translocation of p65 NF-κB subunit into the nucleus and up-regulation of CD88. Inhibition of NF-κB and CD88 using SN50 and W-54011, respectively, resulted in reduced transcription of the IL-8 gene and attenuated secretion of CXCL8 induced by 27OHChol. We propose that oxidatively modified cholesterol like 27OHChol, rather than cholesterol, is responsible for sustained expression of CXCL8 in monocytes/macrophages in atherosclerotic arteries. - Highlights: • Consumption of a high-cholesterol diet leads to increased CXCL8 expression in ApoE{sup −/−} mice. • 27OHChol, but not cholesterol, up-regulates expression of CXCL8 in macrophages. • 27OHChol enhances nuclear translocation of NF-κB and expression of CD88 in macrophages. • Inhibition of NF-κB or CD88 results in decreased CXCL8 expression induced by 27OHChol. • 27OHChol up-regulates CXCL8 expression via NF-κB and CD88 in macrophages.

  10. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  11. PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

    SciTech Connect (OSTI)

    Zheng, Peihao; Guo, Honggang; Li, Guangchao; Han, Siqi; Luo, Fei; Liu, Yi

    2015-03-06

    Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.

  12. Emission factors for domestic use of L.P. gas in the metropolitan area of Mexico City

    SciTech Connect (OSTI)

    Molina, M.M.; Schifter, I.; Ontiveros, L.E.; Salinas, A.; Moreno, S.; Melgarejo, L.A.; Molina, R.; Krueger, B.

    1998-12-31

    One of the main problems found in air pollution in the Metropolitan Area of Mexico City (MAMC) is the presence of high concentrations of ozone at ground level in the atmosphere. The official Mexican standard for ozone concentration in the air (0.11 ppm, one hour, once every 3 years) has been exceeded more than 300 days per year. Ozone is formed due to the emissions of nitrogen oxides and hydrocarbons originated from either combustion processes or vapors emanating from fuel handling operations. The results of an evaluation of several domestic devices like stoves and water heaters with L.P. gas as fuel are presented. A method for the evaluation of hydrocarbon emission was developed. A prototype of domestic installation was constructed. The prototype includes L.P. gas tank, domestic stove, water heater, piping and instrumentation. Several combinations of stoves and water heaters were evaluated. The sampling and analysis of hydrocarbons were performed using laboratory equipment originally designed for the evaluation of combustion and evaporative emissions in automobiles: a SHED camera (sealed room equipped with an hydrocarbon analyzer) was used to measure leaks in the prototype of domestic installation and a Constant Volume Sampler (CVS) for the measurement of incomplete combustion emissions. Emission factors were developed for each domestic installation.

  13. Characterization of Fine Particulate Matter (PM) and Secondary PM Precursor Gases in the Mexico City Metropolitan Area

    DOE R&D Accomplishments [OSTI]

    Molina, Luisa T.; Volkamer, Rainer; de Foy, Benjamin; Lei, Wenfang; Zavala, Miguel; Velasco, Erik; Molina; Mario J.

    2008-10-31

    This project was one of three collaborating grants funded by DOE/ASP to characterize the fine particulate matter (PM) and secondary PM precursors in the Mexico City Metropolitan Area (MCMA) during the MILAGRO Campaign. The overall effort of MCMA-2006, one of the four components, focused on i) examination of the primary emissions of fine particles and precursor gases leading to photochemical production of atmospheric oxidants and secondary aerosol particles; ii) measurement and analysis of secondary oxidants and secondary fine PM production, with particular emphasis on secondary organic aerosol (SOA), and iii) evaluation of the photochemical and meteorological processes characteristic of the Mexico City Basin. The collaborative teams pursued the goals through three main tasks: i) analyses of fine PM and secondary PM precursor gaseous species data taken during the MCMA-2002/2003 campaigns and preparation of publications; ii) planning of the MILAGRO Campaign and deployment of the instrument around the MCMA; and iii) analysis of MCMA-2006 data and publication preparation.

  14. High glucose induces activation of NF-κB inflammatory signaling through IκBα sumoylation in rat mesangial cells

    SciTech Connect (OSTI)

    Huang, Wei; Department of Endocrinology, The People’s Hospital of Xindu, Xindu, Sichuang, 610500 ; Xu, Ling; Zhou, Xueqin; Department of Endocrinology, The People’s Hospital of Leshan, Sichuang ; Gao, Chenlin; Yang, Maojun; Chen, Guo; Zhu, Jianhua; Jiang, Lan; Gan, Huakui; Gou, Fang; Feng, Hong; Peng, Juan; Xu, Yong

    2013-08-30

    Highlights: •The expression of SUMO1, SUMO2/3 under high glucose was obviously enhanced. •High glucose induced degradation of IκBα and activation of NF-κB pathway. •Sumoylation of IκBα in high glucose were significantly decreased. •The proteasome inhibitor MG132 could partially revert the degradation of IκBα. -- Abstract: The posttranslational modification of proteins by small ubiquitin-like modifiers (SUMOs) has emerged as an important regulatory mechanism for the alteration of protein activity, stability, and cellular localization. The latest research demonstrates that sumoylation is extensively involved in the regulation of the nuclear factor κB (NF-κB) pathway, which plays a critical role in the regulation of inflammation and contributes to fibrosis in diabetic nephropathy (DN). However, the role of sumoylation in the regulation of NF-κB signaling in DN is still unclear. In the present study, we cultured rat glomerular mesangial cells (GMCs) stimulated by high glucose and divided GMCs into six groups: normal glucose group (5.6 mmol/L), high glucose groups (10, 20, and 30 mmol/L), mannitol group (i.e., osmotic control group), and MG132 intervention group (30 mmol/L glucose with MG132, a proteasome inhibitor). The expression of SUMO1, SUMO2/3, IκBα, NF-κBp65, and monocyte chemotactic protein 1 (MCP-1) was measured by Western blot, reverse-transcription polymerase chain reaction, and indirect immunofluorescence laser scanning confocal microscopy. The interaction between SUMO1, SUMO2/3, and IκBα was observed by co-immunoprecipitation. The results showed that the expression of SUMO1 and SUMO2/3 was dose- and time-dependently enhanced by high glucose (p < 0.05). However, the expression of IκBα sumoylation in high glucose was significantly decreased compared with the normal glucose group (p < 0.05). The expression of IκBα was dose- and time-dependently decreased, and NF-κBp65 and MCP-1 were increased under high glucose conditions, which

  15. Thermal Reactions of Uranium Metal, UO2, U3O8, UF4, and UO2F2 with NF3 to Produce UF6

    SciTech Connect (OSTI)

    McNamara, Bruce K.; Scheele, Randall D.; Kozelisky, Anne E.; Edwards, Matthew K.

    2009-11-01

    he objective of this paper is to demonstrate that NF3 fluorinates uranium metal, UO2, UF4, UO3, U3O8, and UO2F22H2O to produce the volatile UF6 at temperatures between 100 and 500?C. Thermogravimetric reaction profiles are described that reflect changes in the uranium oxidation state and discrete chemical speciation. Differences in the onset temperatures for each system indicate that NF3-substrate interactions are important for the temperature at which NF3 reacts: U metal > UO3 > UO2 > UO2F2 > UF4 and in fact may indicate different fluorination mechanisms for these various substrates. These studies demonstrate that NF3 is a potential replacement fluorinating agent in the existing nuclear fuel cycle and in oft-proposed actinide volatility reprocessing.

  16. Microstructural evolution of NF709 (20Cr–25Ni–1.5MoNbTiN) under neutron irradiation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kim, Byoungkoo; Tan, Lizhen; Xu, C.; Yang, Yong; Zhang, Xuan; Li, Meimei

    2015-12-30

    In this study, because of its superior creep and corrosion resistance as compared with general austenitic stainless steels, NF709 has emerged as a candidate structural material for advanced nuclear reactors. To obtain fundamental information about the radiation resistance of this material, this study examined the microstructural evolution of NF709 subjected to neutron irradiation to 3 displacements per atom at 500 °C. Transmission electron microscopy, scanning electron microscopy, and high-energy x-ray diffraction were employed to characterize radiation-induced segregation, Frank loops, voids, as well as the formation and reduction of precipitates. Radiation hardening of ~76% was estimated by nanoindentation, approximately consistent withmore » the calculation according to the dispersed barrier-hardening model, suggesting Frank loops as the primary hardening source.« less

  17. Microstructural evolution of NF709 (20Cr–25Ni–1.5MoNbTiN) under neutron irradiation

    SciTech Connect (OSTI)

    Kim, Byoungkoo; Tan, Lizhen; Xu, C.; Yang, Yong; Zhang, Xuan; Li, Meimei

    2015-12-30

    In this study, because of its superior creep and corrosion resistance as compared with general austenitic stainless steels, NF709 has emerged as a candidate structural material for advanced nuclear reactors. To obtain fundamental information about the radiation resistance of this material, this study examined the microstructural evolution of NF709 subjected to neutron irradiation to 3 displacements per atom at 500 °C. Transmission electron microscopy, scanning electron microscopy, and high-energy x-ray diffraction were employed to characterize radiation-induced segregation, Frank loops, voids, as well as the formation and reduction of precipitates. Radiation hardening of ~76% was estimated by nanoindentation, approximately consistent with the calculation according to the dispersed barrier-hardening model, suggesting Frank loops as the primary hardening source.

  18. Subneurotoxic copper(II)-induced NF-κB-dependent microglial activation is associated with mitochondrial ROS

    SciTech Connect (OSTI)

    Hu, Zhuqin; Yu, Fengxiang; Gong, Ping; Qiu, Yu; Zhou, Wei; Cui, Yongyao; Li, Juan Chen, Hongzhuan

    2014-04-15

    Microglia-mediated neuroinflammation and the associated neuronal damage play critical roles in the pathogenesis of neurodegenerative disorders. Evidence shows an elevated concentration of extracellular copper(II) in the brains of these disorders, which may contribute to neuronal death through direct neurotoxicity. Here we explored whether extracellular copper(II) triggers microglial activation. Primary rat microglia and murine microglial cell line BV-2 cells were cultured and treated with copper(II). The content of tumor necrosis factor-α (TNF-α) and nitric oxide in the medium was determined. Extracellular hydrogen peroxide was quantified by a fluorometric assay with Amplex Red. Mitochondrial superoxide was measured by MitoSOX oxidation. At subneurotoxic concentrations, copper(II) treatment induced a dose- and time-dependent release of TNF-α and nitric oxide from microglial cells, and caused an indirect, microglia-mediated neurotoxicity that was blocked by inhibition of TNF-α and nitric oxide production. Copper(II)-initiated microglial activation was accompanied with reduced IkB-α expression as well as phosphorylation and translocation of nuclear factor-κB (NF-κB) p65 and was blocked by NF-κB inhibitors (BAY11-7082 and SC-514). Moreover, copper(II) treatment evoked a rapid release of hydrogen peroxide from microglial cells, an effect that was not affected by NADPH oxidase inhibitors. N-acetyl-cysteine, a scavenger of reactive oxygen species (ROS), abrogated copper(II)-elicited microglial release of TNF-α and nitric oxide and subsequent neurotoxicity. Importantly, mitochondrial production of superoxide, paralleled to extracellular release of hydrogen peroxide, was induced after copper(II) stimulation. Our findings suggest that extracellular copper(II) at subneurotoxic concentrations could trigger NF-κB-dependent microglial activation and subsequent neurotoxicity. NADPH oxidase-independent, mitochondria-derived ROS may be involved in this activation

  19. SENP1 inhibition induces apoptosis and growth arrest of multiple myeloma cells through modulation of NF-κB signaling

    SciTech Connect (OSTI)

    Xu, Jun; Sun, Hui-Yan; Xiao, Feng-Jun; Wang, Hua; Yang, Yang; Wang, Lu; Gao, Chun-Ji; Guo, Zi-Kuan; Wu, Chu-Tse; Wang, Li-Sheng

    2015-05-01

    SUMO/sentrin specific protease 1 (Senp1) is an important regulation protease in the protein sumoylation, which affects the cell cycle, proliferation and differentiation. The role of Senp1 mediated protein desumoylation in pathophysiological progression of multiple myeloma is unknown. In this study, we demonstrated that Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. Lentivirus-mediated Senp1 knockdown triggers apoptosis and reduces viability, proliferation and colony forming ability of MM cells. The NF-κB family members including P65 and inhibitor protein IkBα play important roles in regulation of MM cell survival and proliferation. We further demonstrated that Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation, leading to inactivation of NF-kB signaling in MM cells. These results delineate a key role for Senp1in IL-6 induced proliferation and survival of MM cells, suggesting it may be a potential new therapeutic target in MM. - Highlights: • Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. • Senp1 knockdown triggers apoptosis and reduces proliferation of MM cells. • Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation.

  20. Oxaliplatin antagonizes HIV-1 latency by activating NF-κB without causing global T cell activation

    SciTech Connect (OSTI)

    Zhu, Xiaoli; Liu, Sijie; Wang, Pengfei; Qu, Xiying; Wang, Xiaohui; Zeng, Hanxian; Chen, Huabiao; Zhu, Huanzhang

    2014-07-18

    Highlights: • The chemotherapeutic drug oxaliplatin reactivates latent HIV-1 in this cell line model of HIV-1 latency. • Reactivation is synergized when oxaliplatin is used in combination with valproic acid. • Oxaliplatin reactivates latent HIV-1 through activation of NF-kB and does not induce T cell activation. - Abstract: Reactivation of latent HIV-1 is a promising strategy for the clearance of the viral reservoirs. Because of the limitations of current agents, identification of new latency activators is urgently required. Using an established model of HIV-1 latency, we examined the effect of Oxaliplatin on latent HIV-1 reactivation. We showed that Oxaliplatin, alone or in combination with valproic acid (VPA), was able to reactivate HIV-1 without inducing global T cell activation. We also provided evidence that Oxaliplatin reactivated HIV-1 expression by inducing nuclear factor kappa B (NF-κB) nuclear translocation. Our results indicated that Oxaliplatin could be a potential drug candidate for anti-latency therapies.

  1. Characterization of Fine Particulate Matter (PM) and Secondary PM Precursor Gases in the Mexico City Metropolitan Area

    SciTech Connect (OSTI)

    Molina, Luisa T.; Molina, Mario J.; Volkamer, Rainer; de Foy, Benjamin; Lei, Wenfang; Zavaka, Miguel; Velasco, Erik

    2008-10-31

    This project was one of three collaborating grants funded by DOE/ASP to characterize the fine particulate matter (PM) and secondary PM precursors in the Mexico City Metropolitan Area (MCMA) during the MILAGRO Campaign. The overall effort of MCMA-2006, one of the four components, focused on i) examination of the primary emissions of fine particles and precursor gases leading to photochemical production of atmospheric oxidants and secondary aerosol particles; ii) measurement and analysis of secondary oxidants and secondary fine PM production, with particular emphasis on secondary organic aerosol (SOA), and iii) evaluation of the photochemical and meteorological processes characteristic of the Mexico City Basin. The collaborative teams pursued the goals through three main tasks: i) analyses of fine PM and secondary PM precursor gaseous species data taken during the MCMA-2002/2003 campaigns and preparation of publications; ii) planning of the MILAGRO Campaign and deployment of the instrument around the MCMA; and iii) analysis of MCMA-2006 data and publication preparation. The measurement phase of the MILAGRO Campaign was successfully completed in March 2006 with excellent participation from the international scientific community and outstanding cooperation from the Mexican government agencies and institutions. The project reported here was led by the Massachusetts Institute of Technology/Molina Center for Energy and the Environment (MIT/MCE2) team and coordinated with DOE/ASP-funded collaborators at Aerodyne Research Inc., University of Colorado at Boulder and Montana State University. Currently 24 papers documenting the findings from this project have been published. The results from the project have improved significantly our understanding of the meteorological and photochemical processes contributing to the formation of ozone, secondary aerosols and other pollutants. Key findings from the MCMA-2003 include a vastly improved speciated emissions inventory from on

  2. Urotensin II increases foam cell formation by repressing ABCA1 expression through the ERK/NF-κB pathway in THP-1 macrophages

    SciTech Connect (OSTI)

    Wang, Yan; Wu, Jian-Feng; Tang, Yan-Yan; Zhang, Min; Li, Yuan; Chen, Kong; Zeng, Meng-Ya; Yao, Feng; Xie, Wei; Zheng, Xi-Long; Zeng, Gao-Feng; Tang, Chao-Ke

    2014-10-03

    Highlights: • U II reduces cholesterol efflux in THP-1 macrophages. • U II decreases the expression of ABCA1. • Inhibition of the ERK/NF-κB pathway reduces U II effects on ABCA1 expression and cholesterol efflux. - Abstract: Objective: Foam cell formation in the arterial wall plays a key role in the development of atherosclerosis. Recent studies showed that Urotensin II (U II) is involved in the pathogenesis of atherosclerosis. Here we examined the effects of human U II on ATP-binding cassette transporter A1 (ABCA1) expression and the underlying mechanism in THP-1 macrophages. Methods and results: Cultured THP-1 macrophages were treated with U II, followed by measuring the intracellular lipid contents, cholesterol efflux and ABCA1 levels. The results showed that U II dramatically decreased ABCA1 levels and impaired cholesterol efflux. However, the effects of U II on ABCA1 protein expression and cellular cholesterol efflux were partially reversed by inhibition of extracellular signal regulated kinase 1/2 (ERK1/2) and nuclear factor kappa B (NF-κB) activity, suggesting the potential roles of ERK1/2 and NF-κB in ABCA1 expression, respectively. Conclusion: Our current data indicate that U II may have promoting effects on the progression of atherosclerosis, likely through suppressing ABCA1 expression via activation of the ERK/NF-κB pathway and reducing cholesterol efflux to promote macrophage foam cell formation.

  3. Integrated Genomic Analysis Identifies Clinically Relevant Subtypes of Glioblastoma Characterized by Abnormalities in PDGFRA, IDH1, EGFR, and NF1

    SciTech Connect (OSTI)

    Verhaak, Roel GW; Hoadley, Katherine A; Purdom, Elizabeth; Wang, Victoria; Qi, Yuan; Wilkerson, Matthew D; Miller, C Ryan; Ding, Li; Golub, Todd; Mesirov, Jill P; Alexe, Gabriele; Lawrence, Michael; O'Kelly, Michael; Tamayo, Pablo; Weir, Barbara A; Gabriel, Stacey; Winckler, Wendy; Gupta, Supriya; Jakkula, Lakshmi; Feiler, Heidi S; Hodgson, J Graeme; James, C David; Sarkaria, Jann N; Brennan, Cameron; Kahn, Ari; Spellman, Paul T; Wilson, Richard K; Speed, Terence P; Gray, Joe W; Meyerson, Matthew; Getz, Gad; Perou, Charles M; Hayes, D Neil; Network, The Cancer Genome Atlas Research

    2009-09-03

    The Cancer Genome Atlas Network recently cataloged recurrent genomic abnormalities in glioblastoma multiforme (GBM). We describe a robust gene expression-based molecular classification of GBM into Proneural, Neural, Classical, and Mesenchymal subtypes and integrate multidimensional genomic data to establish patterns of somatic mutations and DNA copy number. Aberrations and gene expression of EGFR, NF1, and PDGFRA/IDH1 each define the Classical, Mesenchymal, and Proneural subtypes, respectively. Gene signatures of normal brain cell types show a strong relationship between subtypes and different neural lineages. Additionally, response to aggressive therapy differs by subtype, with the greatest benefit in the Classical subtype and no benefit in the Proneural subtype. We provide a framework that unifies transcriptomic and genomic dimensions for GBM molecular stratification with important implications for future studies.

  4. Metastatic function of BMP-2 in gastric cancer cells: The role of PI3K/AKT, MAPK, the NF-{kappa}B pathway, and MMP-9 expression

    SciTech Connect (OSTI)

    Kang, Myoung Hee; Oh, Sang Cheul; Kang, Han Na; Kim, Jung Lim; Kim, Jun Suk

    2011-07-15

    Bone morphogenetic proteins (BMPs) have been implicated in tumorigenesis and metastatic progression in various types of cancer cells, but the role and cellular mechanism in the invasive phenotype of gastric cancer cells is not known. Herein, we determined the roles of phosphoinositide 3-kinase (PI3K)/AKT, extracellular signal-regulated protein kinase (ERK), nuclear factor (NF)-{kappa}B, and matrix metalloproteinase (MMP) expression in BMP-2-mediated metastatic function in gastric cancer. We found that stimulation of BMP-2 in gastric cancer cells enhanced the phosphorylation of AKT and ERK. Accompanying activation of AKT and ERK kinase, BMP-2 also enhanced phosphorylation/degradation of I{kappa}B{alpha} and the nuclear translocation/activation of NF-{kappa}B. Interestingly, blockade of PI3K/AKT and ERK signaling using LY294002 and PD98059, respectively, significantly inhibited BMP-2-induced motility and invasiveness in association with the activation of NF-{kappa}B. Furthermore, BMP-2-induced MMP-9 expression and enzymatic activity was also significantly blocked by treatment with PI3K/AKT, ERK, or NF-{kappa}B inhibitors. Immunohistochemistry staining of 178 gastric tumor biopsies indicated that expression of BMP-2 and MMP-9 had a significant positive correlation with lymph node metastasis and a poor prognosis. These results indicate that the BMP-2 signaling pathway enhances tumor metastasis in gastric cancer by sequential activation of the PI3K/AKT or MAPK pathway followed by the induction of NF-{kappa}B and MMP-9 activity, indicating that BMP-2 has the potential to be a therapeutic molecular target to decrease metastasis.

  5. Nuclear NF-?B Expression Correlates With Outcome Among Patients With Head and Neck Squamous Cell Carcinoma Treated With Primary Chemoradiation Therapy

    SciTech Connect (OSTI)

    Balermpas, Panagiotis [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Michel, Yvonne [Senckenberg Institute of Pathology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Senckenberg Institute of Pathology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Wagenblast, Jens [Department of Otorhinolaryngology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Otorhinolaryngology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Seitz, Oliver [Department of Maxillofacial Surgery, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Maxillofacial Surgery, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Sipek, Florian; Rdel, Franz; Rdel, Claus [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Fokas, Emmanouil, E-mail: emmanouil.fokas@kgu.de [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)

    2013-07-15

    Background: To examine whether nuclear NF-?B expression correlates with outcome in patients with head and neck squamous cell carcinoma (HNSCC) treated with primary chemoradiation therapy (CRT). Methods and Materials: Between 2007 and 2010, 101 patients with locally advanced primary HNSCC were treated with definitive simultaneous CRT. Pretreatment biopsy specimens were analyzed for NF-?B p65 (RelA) nuclear immunoreactivity. A sample was assigned to be positive with more than 5% positive nuclear expression. The predictive relevance of NF-?B and clinicopathologic factors for overall survival (OS), progression-free survival (PFS), local progression-free survival (LPFS), and metastasis-free survival (DMFS) was examined by univariate and multivariate analysis. Results: No significant differences between the groups were observed with regard to age, sex, total radiation dose, fractionation mode, total chemotherapy applied, T stage or grading. Patients with p65 nuclear positive biopsy specimens showed significantly a higher rate of lymph node metastasis (cN2c or cN3 status, P=.034). Within a mean follow-up time of 25 months (range, 2.33-62.96 months) OS, PFS, and DMFS were significantly poorer in the p65 nuclear positive group (P=.008, P=.027, and P=.008, respectively). These correlations remained significant in multivariate analysis. Conclusion: NF-?B/p65 nuclear expression is associated with increased lymphatic and hematogenous tumor dissemination and decreased survival in HNSCC patients treated with primary CRT. Our results may foster further investigation of a predictive relevance of NF-?B/p65 and its role as a suitable target for a molecular-based targeted therapy in HNSCC cancer.

  6. Coactivation of the PI3K/Akt and ERK signaling pathways in PCB153-induced NF-κB activation and caspase inhibition

    SciTech Connect (OSTI)

    Liu, Changjiang; Yang, Jixin; Fu, Wenjuan; Qi, Suqin; Wang, Chenmin; Quan, Chao; Yang, Kedi

    2014-06-15

    Polychlorinated biphenyls (PCBs) are a group of persistent and widely distributed environmental pollutants that have various deleterious effects, e.g., neurotoxicity, endocrine disruption and reproductive abnormalities. In order to verify the hypothesis that the PI3K/Akt and MAPK pathways play important roles in hepatotoxicity induced by PCBs, Sprague–Dawley (SD) rats were dosed with PCB153 intraperitoneally at 0, 4, 16 and 32 mg/kg for five consecutive days; BRL cells (rat liver cell line) were treated with PCB153 (0, 1, 5, and 10 μM) for 24 h. Results indicated that the PI3K/Akt and ERK pathways were activated in vivo and in vitro after exposure to PCB153, and protein levels of phospho-Akt and phospho-ERK were significantly increased. Nuclear factor-κB (NF-κB) activation and caspase-3, -8 and -9 inhibition caused by PCB153 were also observed. Inhibiting the ERK pathway significantly attenuated PCB153-induced NF-κB activation, whereas inhibiting the PI3K/Akt pathway hardly influenced phospho-NF-κB level. However, inhibiting the PI3K/Akt pathway significantly elevated caspase-3, -8 and -9 activities, while the ERK pathway only synergistically regulated caspase-9. Proliferating cell nuclear antigen (PCNA), a reliable indicator of cell proliferation, was also induced. Moreover, PCB153 led to hepatocellular hypertrophy and elevated liver weight. Taken together, PCB153 leads to aberrant proliferation and apoptosis of hepatocytes through NF-κB activation and caspase inhibition, and coactivated PI3K/Akt and ERK pathways play critical roles in PCB153-induced hepatotoxicity. - Highlights: • PCB153 led to hepatotoxicity through NF-κB activation and caspase inhibition. • The PI3K/Akt and ERK pathways were coactivated in vivo and in vitro by PCB153. • The ERK pathway regulated levels of phospho-NF-κB and caspase-9. • The PI3K/Akt pathway regulated levels of caspase-3, -8 and -9.

  7. A Case Study of Urbanization Impact on Summer Precipitation in the Greater Beijing Metropolitan Area. Urban Heat Island Versus Aerosol Effects

    SciTech Connect (OSTI)

    Zhong, Shi; Qian, Yun; Zhao, Chun; Leung, Lai-Yung R.; Yang, Xiuqun

    2015-10-23

    Convection-resolving ensemble simulations using the WRF-Chem model coupled with a single-layer Urban Canopy Model (UCM) are conducted to investigate the individual and combined impacts of land use and anthropogenic pollutant emissions from urbanization on a heavy rainfall event in the Greater Beijing Metropolitan Area (GBMA) in China. The simulation with the urbanization effect included generally captures the spatial pattern and temporal variation of the rainfall event. An improvement of precipitation is found in the experiment including aerosol effect on both clouds and radiation. The expanded urban land cover and increased aerosols have an opposite effect on precipitation processes, with the latter playing a more dominant role, leading to suppressed convection and rainfall over the upstream (northwest) area, and enhanced convection and more precipitation in the downstream (southeast) region of the GBMA. In addition, the influence of aerosol indirect effect is found to overwhelm that of direct effect on precipitation in this rainfall event. Increased aerosols induce more cloud droplets with smaller size, which favors evaporative cooling and reduce updrafts and suppress convection over the upstream (northwest) region in the early stage of the rainfall event. As the rainfall system propagates southeastward, more latent heat is released due to the freezing of larger number of smaller cloud drops that are lofted above the freezing level, which is responsible for the increased updraft strength and convective invigoration over the downstream (southeast) area.

  8. Gastrodin stimulates anticancer immune response and represses transplanted H22 hepatic ascitic tumor cell growth: Involvement of NF-?B signaling activation in CD4 + T cells

    SciTech Connect (OSTI)

    Shu, Guangwen; Yang, Tianming; Wang, Chaoyuan; Su, Hanwen; Xiang, Meixian

    2013-06-15

    Gastrodia elata Blume (G. elata) is a famous restorative food in East Asia. It can be used as an auxiliary reagent in hepatocellular carcinoma (HCC) treatment. Previous studies unveiled that G. elata exhibited immunomodulatory activities. To explore the active ingredients contributing to its immunomodulatory activities, gastrodin, vanillin, and parishin B were purified from G. elata and their anti-HCC effects were assessed in vivo. Among these compounds, only gastrodin was capable of repressing transplanted H22 ascitic hepatic tumor cell growth in vivo with low toxicity. Further investigations were designed to explore the effects of gastrodin on the immune system of tumor-bearing mice and potential molecular mechanisms underlying these effects. Our data showed that gastrodin ameliorated tumor cell transplantation-induced activation of endogenous pro-apoptotic pathway in CD4 + T cells and abnormalities in serum cytokine profiles in host animals. These events enhanced cytotoxic activities of natural killer and CD8 + T cells against H22 hepatic cancer cells. Gastrodin administration specifically upregulated mRNA levels of several nuclear factor ?B (NF-?B) responsive genes in CD4 + T cells but not in CD8 + T cells. Chromatin immunoprecipitation assay showed that gastrodin increased the association of NF-?B p65 subunit to the promoter regions of IL-2 and Bcl-2 encoding genes in CD4 + T cells. Our investigations demonstrated that gastrodin is the main active ingredient contributing to the anticancer immunomodulatory properties of G. elata. Promoting NF-?B-mediated gene transcription in CD4 + T cells is implicated in its immunomodulatory activity. - Highlights: Gastrodin stimulates anticancer immune response. Gastrodin represses tumor transplantation-induced CD4 + T cell apoptosis. Gastrodin activates NF-?B activity in CD4 + T cells.

  9. Moderate extracellular acidification inhibits capsaicin-induced cell death through regulating calcium mobilization, NF-{kappa}B translocation and ROS production in synoviocytes

    SciTech Connect (OSTI)

    Hu, Fen; Yang, Shuang; Zhao, Dan; Zhu, Shuyan; Wang, Yuxiang; Li, Junying

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Moderate extracellular acidification regulates intracellular Ca{sup 2+} mobilization. Black-Right-Pointing-Pointer Moderate acidification activates NF-{kappa}B nuclear translocation in synoviocytes. Black-Right-Pointing-Pointer Moderate acidification depresses the ROS production induced by capsaicin. Black-Right-Pointing-Pointer Moderate acidification inhibits capsaicin-caused synoviocyte death. -- Abstract: We previously show the expression of transient receptor potential vanilloid 1 (TRPV1) in primary synoviocytes from collagen-induced arthritis (CIA) rats. Capsaicin and lowered extracellular pH from 7.4 to 5.5 induce cell death through TRPV1-mediated Ca{sup 2+} entry and reactive oxygen species (ROS) production. However, under the pathological condition in rheumatoid arthritis, the synovial fluid is acidified to a moderate level (about pH 6.8). In the present study, we examined the effects of pH 6.8 on the TRPV1-mediated cell death. Our finding is different or even opposite from what was observed at pH 5.5. We found that the moderate extracellular acidification (from pH 7.4 to 6.8) inhibited the capsaicin-induced Ca{sup 2+} entry through attenuating the activity of TRPV1. In the mean time, it triggered a phospholipse C (PLC)-related Ca{sup 2+} release from intracellular stores. The nuclear translocation of NF-{kappa}B was found at pH 6.8, and this also depends on PLC activation. Moreover, the capsaicin-evoked massive ROS production and cell death were depressed at pH 6.8, both of which are dependent on the activation of PLC and NF-{kappa}B. Taken together, these results suggested that the moderate extracellular acidification inhibited the capsaicin-induced synoviocyte death through regulating Ca{sup 2+} mobilization, activating NF-{kappa}B nuclear translocation and depressing ROS production.

  10. Stevioside ameliorates high-fat diet-induced insulin resistance and adipose tissue inflammation by downregulating the NF-{kappa}B pathway

    SciTech Connect (OSTI)

    Wang, Zhiquan; Xue, Liqiong; Guo, Cuicui; Han, Bing; Pan, Chunming; Zhao, Shuangxia; Song, Huaidong; Ma, Qinyun

    2012-01-27

    Highlights: Black-Right-Pointing-Pointer Stevioside ameliorates high-fat diet-induced insulin resistance. Black-Right-Pointing-Pointer Stevioside alleviates the adipose tissue inflammation. Black-Right-Pointing-Pointer Stevioside reduces macrophages infiltration into the adipose tissue. Black-Right-Pointing-Pointer Stevioside suppresses the activation of NF-{kappa}B in the adipose tissue. -- Abstract: Accumulating evidence suggests that adipose tissue is the main source of pro-inflammatory molecules that predispose individuals to insulin resistance. Stevioside (SVS) is a widely used sweetener with multiple beneficial effects for diabetic patients. In this study, we investigated the effect of SVS on insulin resistance and the pro-inflammatory state of adipose tissue in mice fed with a high-fat diet (HFD). Oral administration of SVS for 1 month had no effect on body weight, but it significantly improved fasting glucose, basal insulin levels, glucose tolerance and whole body insulin sensitivity. Interestingly, these changes were accompanied with decreased expression levels of several inflammatory cytokines in adipose tissue, including TNF-{alpha}, IL6, IL10, IL1{beta}, KC, MIP-1{alpha}, CD11b and CD14. Moreover, macrophage infiltration in adipose tissue was remarkably reduced by SVS. Finally, SVS significantly suppressed the nuclear factor-kappa b (NF-{kappa}B) signaling pathway in adipose tissue. Collectively, these results suggested that SVS may ameliorate insulin resistance in HFD-fed mice by attenuating adipose tissue inflammation and inhibiting the NF-{kappa}B pathway.

  11. ROS and NF-{kappa}B are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes

    SciTech Connect (OSTI)

    Ye Shefang Wu Yihui; Hou Zhenqing; Zhang Qiqing

    2009-02-06

    Carbon nanotubes (CNTs) have potential applications in biosensors, tissue engineering, and biomedical devices because of their unique physico-chemical, electronic and mechanical properties. However, there is limited literature data available concerning the biological properties and toxicity of CNTs. This study aimed to assess the toxicity exhibited by multi-walled CNTs (MWCNTs) and to elucidate possible molecular mechanisms underlying the biological effects of MWCNTs in A549 cells. Exposing A549 cells to MWCNTs led to cell death, changes in cell size and complexity, reactive oxygen species (ROS) production, interleukin-8 (IL-8) gene expression and nuclear factor (NF)-{kappa}B activation. Treatment of A549 cells with antioxidants prior to adding MWCNTs decreased ROS production and abrogated expression of IL-8 mRNA. Pretreatment of A549 cells with NF-{kappa}B inhibitors suppressed MWCNTs-induced IL-8 mRNA expression. These results indicate that MWCNTs are able to induce expression of IL-8 in A549 cells, at least in part, mediated by oxidative stress and NF-{kappa}B activation.

  12. Neutron and proton electric dipole moments from Nf=2+1 domain-wall fermion lattice QCD

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shintani, Eigo; Blum, Thomas; Izubuchi, Taku; Soni, Amarjit

    2016-05-05

    We present a lattice calculation of the neutron and proton electric dipole moments (EDM’s) with Nf = 2 + 1 flavors of domain-wall fermions. The neutron and proton EDM form factors are extracted from three-point functions at the next-to-leading order in the θ vacuum of QCD. In this computation, we use pion masses 330 and 420 MeV and 2.7 fm3 lattices with Iwasaki gauge action and a 170 MeV pion and 4.6 fm3 lattice with I-DSDR gauge action, all generated by the RBC and UKQCD collaborations. The all-mode-averaging technique enables an efficient, high statistics calculation; however the statistical errors onmore » our results are still relatively large, so we investigate a new direction to reduce them, reweighting with the local topological charge density which appears promising. Furthermore, we discuss the chiral behavior and finite size effects of the EDM’s in the context of baryon chiral perturbation theory.« less

  13. Ascorbic acid suppresses endotoxemia and NF-κB signaling cascade in alcoholic liver fibrosis in guinea pigs: A mechanistic approach

    SciTech Connect (OSTI)

    Abhilash, P.A.; Harikrishnan, R.; Indira, M.

    2014-01-15

    Alcohol consumption increases the small intestinal bacterial overgrowth (SIBO) and intestinal permeability of endotoxin. The endotoxin mediated inflammatory signaling plays a major role in alcoholic liver fibrosis. We evaluated the effect of ascorbic acid (AA), silymarin and alcohol abstention on the alcohol induced endotoxemia and NF-κB activation cascade pathway in guinea pigs (Cavia porcellus). Guinea pigs were administered ethanol at a daily dose of 4 g/kg b.wt for 90 days. After 90 days, ethanol administration was stopped. The ethanol treated animals were divided into abstention, silymarin (250 mg/kg b.wt) and AA (250 mg/kg b.wt) supplemented groups and maintained for 30 days. The SIBO, intestinal permeability and endotoxin were significantly increased in the ethanol group. The mRNA expressions of intestinal proteins claudin, occludin and zona occludens-1 were significantly decreased in ethanol group. The mRNA levels of inflammatory receptors, activity of IKKβ and the protein expressions of phospho-IκBα, NF-κB, TNF-α, TGF-β{sub 1} and IL-6 were also altered in ethanol group. The expressions of fibrosis markers α-SMA, α{sub 1} (I) collagen and sirius red staining in the liver revealed the induction of fibrosis. But the supplementation of AA could induce greater reduction of ethanol induced SIBO, intestinal barrier defects, NF-κB activation and liver fibrosis than silymarin. The possible mechanism may be the inhibitory effect of AA on SIBO, intestinal barrier defect and IKKβ, which decreased the activation of NF-κB and synthesis of cytokines. This might have led to suppression of HSCs activation and liver fibrosis. - Highlights: • Alcohol increases intestinal bacterial overgrowth and permeability of endotoxin. • Endotoxin mediated inflammation plays a major role in alcoholic liver fibrosis. • Ascorbic acid reduces endotoxemia, NF-κB activation and proinflammatory cytokines. • AA's action is by inhibition of SIBO, IKKβ and alteration of

  14. Tat-CBR1 inhibits inflammatory responses through the suppressions of NF-κB and MAPK activation in macrophages and TPA-induced ear edema in mice

    SciTech Connect (OSTI)

    Kim, Young Nam; Kim, Dae Won; Jo, Hyo Sang; Shin, Min Jea; Ahn, Eun Hee; Ryu, Eun Ji; Yong, Ji In; Cha, Hyun Ju; Kim, Sang Jin; Yeo, Hyeon Ji; Youn, Jong Kyu; Hwang, Jae Hyeok; Jeong, Ji-Heon; Kim, Duk-Soo; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

    2015-07-15

    Human carbonyl reductase 1 (CBR1) plays a crucial role in cell survival and protects against oxidative stress response. However, its anti-inflammatory effects are not yet clearly understood. In this study, we examined whether CBR1 protects against inflammatory responses in macrophages and mice using a Tat-CBR1 protein which is able to penetrate into cells. The results revealed that purified Tat-CBR1 protein efficiently transduced into Raw 264.7 cells and inhibited lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2), nitric oxide (NO) and prostaglandin E{sub 2} (PGE{sub 2}) expression levels. In addition, Tat-CBR1 protein leads to decreased pro-inflammatory cytokine expression through suppression of nuclear transcription factor-kappaB (NF-κB) and mitogen activated protein kinase (MAPK) activation. Furthermore, Tat-CBR1 protein inhibited inflammatory responses in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation when applied topically. These findings indicate that Tat-CBR1 protein has anti-inflammatory properties in vitro and in vivo through inhibition of NF-κB and MAPK activation, suggesting that Tat-CBR1 protein may have potential as a therapeutic agent against inflammatory diseases. - Highlights: • Transduced Tat-CBR1 reduces LPS-induced inflammatory mediators and cytokines. • Tat-CBR1 inhibits MAPK and NF-κB activation. • Tat-CBR1 ameliorates inflammation response in vitro and in vivo. • Tat-CBR1 may be useful as potential therapeutic agent for inflammation.

  15. The plant limonoid 7-oxo-deacetoxygedunin inhibits RANKL-induced osteoclastogenesis by suppressing activation of the NF-{kappa}B and MAPK pathways

    SciTech Connect (OSTI)

    Wisutsitthiwong, Chonnaree; Buranaruk, Chayanit; Pudhom, Khanitha; Palaga, Tanapat

    2011-11-18

    Highlights: Black-Right-Pointing-Pointer A gedunin type limonoid from seeds of mangroves, 7-oxo-7-deacetoxygedunin, exhibits strong anti-osteoclastogenic activity. Black-Right-Pointing-Pointer Treatment with this limonoid results in significant decrease in expression of NFATc1 and osteoclast-related genes. Black-Right-Pointing-Pointer The mode of action of this limonoid is by inhibiting activation of the NF-{kappa}B and MAPK pathways which are activated by RANKL. -- Abstract: Osteoclasts together with osteoblasts play pivotal roles in bone remodeling. Aberrations in osteoclast differentiation and activity contribute to osteopenic disease. Osteoclasts differentiate from monocyte/macrophage progenitors, a process that is initiated by the interaction between receptor activator of NF-{kappa}B (RANK) and its ligand, RANKL. In this study, we identified 7-oxo-7-deacetoxygedunin (7-OG), a gedunin type limonoid from seeds of the mangrove Xylocarpus moluccensis, as a potent inhibitor of osteoclastogenesis. Additionally, 7-OG showed strong anti-osteoclastogenic activity with low cytotoxicity against the monocyte/macrophage progenitor cell line, RAW264.7. The IC50 for anti-osteoclastogenic activity was 4.14 {mu}M. Treatment with 7-OG completely abolished the appearance of multinucleated giant cells with tartrate-resistant acid phosphatase activity in RAW264.7 cells stimulated with RANKL. When the expression of genes related to osteoclastogenesis was investigated, a complete downregulation of NFATc1 and cathepsin K and a delayed downregulation of irf8 were observed upon 7-OG treatment in the presence of RANKL. Furthermore, treatment with this limonoid suppressed RANKL-induced activation of p38, MAPK and Erk and nuclear localization of NF-{kappa}B p65. Taken together, we present evidence indicating a plant limonoid as a novel osteoclastogenic inhibitor that could be used for osteoporosis and related conditions.

  16. Mangiferin exerts hepatoprotective activity against D-galactosamine induced acute toxicity and oxidative/nitrosative stress via Nrf2NF?B pathways

    SciTech Connect (OSTI)

    Das, Joydeep; Ghosh, Jyotirmoy; Roy, Anandita; Sil, Parames C.

    2012-04-01

    Mangiferin, a xanthone glucoside, is well known to exhibit antioxidant, antiviral, antitumor, anti-inflammatory and gene-regulatory effects. In the present study, we isolated mangiferin from the bark of Mangifera indica and assessed its beneficial role in galactosamine (GAL) induced hepatic pathophysiology. GAL (400 mg/kg body weight) exposed hepatotoxic rats showed elevation in the activities of serum ALP, ALT, levels of triglycerides, total cholesterol, lipid-peroxidation and reduction in the levels of serum total proteins, albumin and cellular GSH. Besides, GAL exposure (5 mM) in hepatocytes induced apoptosis and necrosis, increased ROS and NO production. Signal transduction studies showed that GAL exposure significantly increased the nuclear translocation of NF?B and elevated iNOS protein expression. The same exposure also elevated TNF-?, IFN-?, IL-1?, IL-6, IL-12, IL-18 and decreased IL-10 mRNA expressions. Furthermore, GAL also decreased the protein expression of Nrf2, NADPH:quinine oxidoreductase-1, heme oxygenase-1 and GST?. However, mangiferin administration in GAL intoxicated rats or coincubation of hepatocytes with mangiferin significantly altered all these GAL-induced adverse effects. In conclusion, the hepatoprotective role of mangiferin was due to induction of antioxidant defense via the Nrf2 pathway and reduction of inflammation via NF?B inhibition. Highlights: ?Galactosamine induces hepatocytes death via oxidative and nitrosative stress. ?Mangiferin exerts hepatoprotective effect/antioxidant defense via Nrf2 pathway. ?Mangiferin exerts anti-inflammatory responses by inhibiting NF-?B. ?Mangiferin suppresses galactosamine-induced repression of IL-10 mRNA.

  17. HSF1 and NF-κB p65 participate in the process of exercise preconditioning attenuating pressure overload-induced pathological cardiac hypertrophy

    SciTech Connect (OSTI)

    Xu, Tongyi; Zhang, Ben; Yang, Fan; Cai, Chengliang; Wang, Guokun; Han, Qingqi; Zou, Liangjian

    2015-05-08

    Pathological cardiac hypertrophy, often accompanied by hypertension, aortic stenosis and valvular defects, is typically associated with myocyte remodeling and cardiac dysfunction. Exercise preconditioning (EP) has been proven to enhance the tolerance of the myocardium to cardiac ischemia-reperfusion injury. However, the effects of EP in pathological cardiac hypertrophy are rarely reported. 10-wk-old male Sprague–Dawley rats (n = 80) were randomly divided into four groups: sham, TAC, EP + sham and EP + TAC. Two EP groups were subjected to 4 weeks of treadmill training, and the EP + TAC and TAC groups were followed by TAC operations. The sham and EP + sham groups underwent the same operation without aortic constriction. Eight weeks after the surgery, we evaluated the effects of EP by echocardiography, morphology, and histology and observed the expressions of the associated proteins. Compared with the respective control groups, hypertrophy-related indicators were significantly increased in the TAC and EP + TAC groups (p < 0.05). However, between the TAC and EP + TAC groups, all of these changes were effectively inhibited by EP treatment (p < 0.05). Furthermore, EP treatment upregulated the expression of HSF1 and HSP70, increased the HSF1 levels in the nuclear fraction, inhibited the expression of the NF-κB p65 subunit, decreased the NF-κB p65 subunit levels in the nuclear fraction, and reduced the IL2 levels in the myocardia of rats. EP could effectively reduce the cardiac hypertrophic responses induced by TAC and may play a protective role by upregulating the expressions of HSF1 and HSP70, activating HSF1 and then inhibiting the expression of NF-κB p65 and nuclear translocation. - Highlights: • EP could effectively reduce the cardiac hypertrophic responses induced by TAC. • EP may play a protective role by upregulating the expressions of HSF1 and HSP70 and then activating HSF1. • EP may play a protective role by inhibiting the expression

  18. OSU-A9 inhibits angiogenesis in human umbilical vein endothelial cells via disrupting AktNF-?B and MAPK signaling pathways

    SciTech Connect (OSTI)

    Omar, Hany A.; Arafa, El-Shaimaa A.; Salama, Samir A.; Arab, Hany H.; Wu, Chieh-Hsi; Weng, Jing-Ru

    2013-11-01

    Since the introduction of angiogenesis as a useful target for cancer therapy, few agents have been approved for clinical use due to the rapid development of resistance. This problem can be minimized by simultaneous targeting of multiple angiogenesis signaling pathways, a potential strategy in cancer management known as polypharmacology. The current study aimed at exploring the anti-angiogenic activity of OSU-A9, an indole-3-carbinol-derived pleotropic agent that targets mainly Aktnuclear factor-kappa B (NF-?B) signaling which regulates many key players of angiogenesis such as vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Human umbilical vein endothelial cells (HUVECs) were used to study the in vitro anti-angiogenic effect of OSU-A9 on several key steps of angiogenesis. Results showed that OSU-A9 effectively inhibited cell proliferation and induced apoptosis and cell cycle arrest in HUVECs. Besides, OSU-A9 inhibited angiogenesis as evidenced by abrogation of migration/invasion and Matrigel tube formation in HUVECs and attenuation of the in vivo neovascularization in the chicken chorioallantoic membrane assay. Mechanistically, Western blot, RT-PCR and ELISA analyses showed the ability of OSU-A9 to inhibit MMP-2 production and VEGF expression induced by hypoxia or phorbol-12-myristyl-13-acetate. Furthermore, dual inhibition of AktNF-?B and mitogen-activated protein kinase (MAPK) signaling, the key regulators of angiogenesis, was observed. Together, the current study highlights evidences for the promising anti-angiogenic activity of OSU-A9, at least in part through the inhibition of AktNF-?B and MAPK signaling and their consequent inhibition of VEGF and MMP-2. These findings support OSU-A9's clinical promise as a component of anticancer therapy. - Highlights: The antiangiogenic activity of OSU-A9 in HUVECs was explored. OSU-A9 inhibited HUVECs proliferation, migration, invasion and tube formation. OSU-A9 targeted signaling

  19. Agent-based model forecasts aging of the population of people who inject drugs in metropolitan Chicago and changing prevalence of hepatitis C infections

    SciTech Connect (OSTI)

    Gutfraind, Alexander; Boodram, Basmattee; Prachand, Nikhil; Hailegiorgis, Atesmachew; Dahari, Harel; Major, Marian E.; Kaderali, Lars

    2015-09-30

    People who inject drugs (PWID) are at high risk for blood-borne pathogens transmitted during the sharing of contaminated injection equipment, particularly hepatitis C virus (HCV). HCV prevalence is influenced by a complex interplay of drug-use behaviors, social networks, and geography, as well as the availability of interventions, such as needle exchange programs. To adequately address this complexity in HCV epidemic forecasting, we have developed a computational model, the Agent-based Pathogen Kinetics model (APK). APK simulates the PWID population in metropolitan Chicago, including the social interactions that result in HCV infection. We used multiple empirical data sources on Chicago PWID to build a spatial distribution of an in silico PWID population and modeled networks among the PWID by considering the geography of the city and its suburbs. APK was validated against 2012 empirical data (the latest available) and shown to agree with network and epidemiological surveys to within 1%. For the period 2010–2020, APK forecasts a decline in HCV prevalence of 0.8% per year from 44(±2)% to 36(±5)%, although some sub-populations would continue to have relatively high prevalence, including Non-Hispanic Blacks, 48(±5)%. The rate of decline will be lowest in Non-Hispanic Whites and we find, in a reversal of historical trends, that incidence among non-Hispanic Whites would exceed incidence among Non-Hispanic Blacks (0.66 per 100 per years vs 0.17 per 100 person years). APK also forecasts an increase in PWID mean age from 35(±1) to 40(±2) with a corresponding increase from 59(±2)% to 80(±6)% in the proportion of the population >30 years old. Our research highlight the importance of analyzing sub-populations in disease predictions, the utility of computer simulation for analyzing demographic and health trends among PWID and serve as a tool for guiding intervention and prevention strategies in Chicago, and other major cities.

  20. Agent-based model forecasts aging of the population of people who inject drugs in metropolitan Chicago and changing prevalence of hepatitis C infections

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Gutfraind, Alexander; Boodram, Basmattee; Prachand, Nikhil; Hailegiorgis, Atesmachew; Dahari, Harel; Major, Marian E.; Kaderali, Lars

    2015-09-30

    People who inject drugs (PWID) are at high risk for blood-borne pathogens transmitted during the sharing of contaminated injection equipment, particularly hepatitis C virus (HCV). HCV prevalence is influenced by a complex interplay of drug-use behaviors, social networks, and geography, as well as the availability of interventions, such as needle exchange programs. To adequately address this complexity in HCV epidemic forecasting, we have developed a computational model, the Agent-based Pathogen Kinetics model (APK). APK simulates the PWID population in metropolitan Chicago, including the social interactions that result in HCV infection. We used multiple empirical data sources on Chicago PWID tomore » build a spatial distribution of an in silico PWID population and modeled networks among the PWID by considering the geography of the city and its suburbs. APK was validated against 2012 empirical data (the latest available) and shown to agree with network and epidemiological surveys to within 1%. For the period 2010–2020, APK forecasts a decline in HCV prevalence of 0.8% per year from 44(±2)% to 36(±5)%, although some sub-populations would continue to have relatively high prevalence, including Non-Hispanic Blacks, 48(±5)%. The rate of decline will be lowest in Non-Hispanic Whites and we find, in a reversal of historical trends, that incidence among non-Hispanic Whites would exceed incidence among Non-Hispanic Blacks (0.66 per 100 per years vs 0.17 per 100 person years). APK also forecasts an increase in PWID mean age from 35(±1) to 40(±2) with a corresponding increase from 59(±2)% to 80(±6)% in the proportion of the population >30 years old. Our research highlight the importance of analyzing sub-populations in disease predictions, the utility of computer simulation for analyzing demographic and health trends among PWID and serve as a tool for guiding intervention and prevention strategies in Chicago, and other major cities.« less

  1. TGF-{beta}1 increases invasiveness of SW1990 cells through Rac1/ROS/NF-{kappa}B/IL-6/MMP-2

    SciTech Connect (OSTI)

    Binker, Marcelo G.; CBRHC Research Center, Buenos Aires ; Binker-Cosen, Andres A.; Gaisano, Herbert Y.; Cosen, Rodica H. de; Cosen-Binker, Laura I.

    2011-02-04

    Research highlights: {yields} Rac1 mediates TGF-{beta}1-induced SW1990 invasion through MMP-2 secretion and activation. {yields} NADPH-generated ROS act downstream of Rac1 in TGF-{beta}1-challenged SW1990 cells. {yields} TGF-{beta}1-stimulated ROS activate NF-{kappa}B in SW1990 cells. {yields} NF{kappa}B-induced IL-6 release is required for secretion and activation of MMP-2 in SW1990 cells. -- Abstract: Human pancreatic cancer invasion and metastasis have been found to correlate with increased levels of active matrix metalloproteinase 2 (MMP-2). The multifunctional cytokine transforming growth factor beta 1 (TGF-{beta}1) has been shown to increase both secretion of MMP-2 and invasion by several pancreatic cancer cell types. In the present study, we investigated the signaling pathway involved in TGF-{beta}1-promoted MMP-2 secretion and invasion by human pancreatic cancer cells SW1990. Using specific inhibitors, we found that stimulation of these tumor cells with TGF-{beta}1 induced secretion and activation of the collagenase MMP-2, which was required for TGF-{beta}1-stimulated invasion. Our results also indicate that signaling events involved in TGF-{beta}1-enhanced SW1990 invasiveness comprehend activation of Rac1 followed by generation of reactive oxygen species through nicotinamide adenine dinucleotide phosphate-oxidase, activation of nuclear factor-kappa beta, release of interleukin-6, and secretion and activation of MMP-2.

  2. Cyanidin-3-glucoside inhibits UVB-induced oxidative damage and inflammation by regulating MAP kinase and NF-κB signaling pathways in SKH-1 hairless mice skin

    SciTech Connect (OSTI)

    Pratheeshkumar, Poyil; Son, Young-Ok; Wang, Xin; Divya, Sasidharan Padmaja; Joseph, Binoy; Hitron, John Andrew; Wang, Lei; Kim, Donghern; Yin, Yuanqin; Roy, Ram Vinod; Lu, Jian; Zhang, Zhuo; Wang, Yitao; and others

    2014-10-01

    Skin cancer is one of the most commonly diagnosed cancers in the United States. Exposure to ultraviolet-B (UVB) radiation induces inflammation and photocarcinogenesis in mammalian skin. Cyanidin-3-glucoside (C3G), a member of the anthocyanin family, is present in various vegetables and fruits especially in edible berries, and displays potent antioxidant and anticarcinogenic properties. In this study, we have assessed the in vivo effects of C3G on UVB irradiation induced chronic inflammatory responses in SKH-1 hairless mice, a well-established model for UVB-induced skin carcinogenesis. Here, we show that C3G inhibited UVB-induced skin damage and inflammation in SKH-1 hairless mice. Our results indicate that C3G inhibited glutathione depletion, lipid peroxidation and myeloperoxidation in mouse skin by chronic UVB exposure. C3G significantly decreased the production of UVB-induced pro-inflammatory cytokines, such as IL-6 and TNF-α, associated with cutaneous inflammation. Likewise, UVB-induced inflammatory responses were diminished by C3G as observed by a remarkable reduction in the levels of phosphorylated MAP kinases, Erk1/2, p38, JNK1/2 and MKK4. Furthermore, C3G also decreased UVB-induced cyclooxygenase-2 (COX-2), PGE{sub 2} and iNOS levels, which are well-known key mediators of inflammation and cancer. Treatment with C3G inhibited UVB-induced nuclear translocation of NF-κB and degradation of IκBα in mice skin. Immunofluorescence assay revealed that topical application of C3G inhibited the expression of 8-hydroxy-2′-deoxyguanosine, proliferating cell nuclear antigen, and cyclin D1 in chronic UVB exposed mouse skin. Collectively, these data indicates that C3G can provide substantial protection against the adverse effects of UVB radiation by modulating UVB-induced MAP kinase and NF-κB signaling pathways. - Highlights: • C3G inhibited UVB-induced oxidative damage and inflammation. • C3G inhibited UVB-induced COX-2, iNOS and PGE{sub 2} production. • C3G

  3. The (3He,tf) as a surrogate reaction to determine (n,f) cross sections in the 10 to 20 MeV energy range

    SciTech Connect (OSTI)

    Basunia, M. S.; Clark, R. M.; Goldblum, B. L.; Bernstein, L. A.; Phair, L.; Burke, J. T.; Beausang, C. W.; Bleuel, D. L.; Darakchieva, B.; Dietrich, F. S.; Evtimova, M.; Fallon, P.; Gibelin, J.; Hatarik, R.; Jewett, C. C.; Lesher, S. R.; McMahan, M. A.; Rodriguez-Vieitez, E.; Wiedeking, M.

    2009-02-25

    The surrogate reaction 238U(3He,tf) is used to determine the 237Np(n,f) cross section indirectly over an equivalent neutron energy range from 10 to 20 MeV. A self-supporting ~;;761 mu g/cm2 metallic 238U foil was bombarded with a 42 MeV 3He2+ beam from the 88-Inch Cyclotron at Lawrence Berkeley National Laboratory (LBNL). Outgoing charged particles and fission fragments were identified using the Silicon Telescope Array for Reaction Studies (STARS), consists of two 140 mu m and one 1000 mu m Micron S2 type silicon detectors. The 237Np(n,f) cross sections, determined indirectly, were compared with the 237Np(n,f) cross section data from direct measurements, the Evaluated Nuclear Data File (ENDF/B-VII.0), and the Japanese Evaluated Nuclear Data Library (JENDL 3.3) and found to closely follow those datasets. Use of the (3He,tf) reaction as a surrogate to extract (n,f) cross section in the 10 to 20 MeV equivalent neutron energy is found to be suitable.

  4. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  5. The REMOTE SENSlNf

    Office of Legacy Management (LM)

    ... Nuclear Safety 20:736-47. Beck, H. L.; De Campo, J.; and Gogolak, C. 1972. In Situ Ge (Li) and N@ fT) Gamma Ray Spectrometry. HASL-258. (United States Atomic Energy CornmIssIon, ...

  6. Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-κB activation

    SciTech Connect (OSTI)

    Yun, Hong Shik; Baek, Jeong-Hwa; Yim, Ji-Hye; Lee, Su-Jae; Lee, Chang-Woo; Song, Jie-Young; Um, Hong-Duck; Park, Jong Kuk; Park, In-Chul; Hwang, Sang-Gu

    2014-07-11

    Highlights: • HRP-3 is a radiation- and anticancer drug-responsive protein in H1299 cells. • Depletion of HRP-3 induces apoptosis of radio- and chemoresistant H1299 cells. • Depletion of HRP-3 promotes ROS generation via inhibition of the Nrf2/HO-1 pathway. • ROS generation enhances NF-κB activity, which acts as an upstream signal in the c-Myc/Noxa apoptotic pathway. - Abstract: We previously identified hepatoma-derived growth factor-related protein-3 (HRP-3) as a radioresistant biomarker in p53 wild-type A549 cells and found that p53-dependent induction of the PUMA pathway was a critical event in regulating the radioresistant phenotype. Here, we found that HRP-3 knockdown regulates the radioresistance of p53-null H1299 cells through a distinctly different molecular mechanism. HRP-3 depletion was sufficient to cause apoptosis of H1299 cells by generating substantial levels of reactive oxygen species (ROS) through inhibition of the Nrf2/HO-1 antioxidant pathway. Subsequent, ROS-dependent and p53-independent NF-κB activation stimulated expression of c-Myc and Noxa proteins, thereby inducing the apoptotic machinery. Our results thus extend the range of targets for the development of new drugs to treat both p53 wild-type or p53-null radioresistant lung cancer cells.

  7. Alternative Fuels Data Center: Metropolitan Utilities District...

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    ... Regional Heavy-Duty LNG Fueling Station March 21, 2015 Photo of a street sweeper New Hampshire Fleet Revs up With Natural Gas March 7, 2015 Photo of a truck pulling into a CNG ...

  8. Climate Action Champions: Metropolitan Washington Council of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    baseline regional greenhouse gas inventory, examines potential climate change impacts, evaluates mitigation and adaptation strategies, and establishes greenhouse gas emission ...

  9. Metropolitan Edison Company SEF Grants (FirstEnergy Territory)

    Broader source: Energy.gov [DOE]

    Examples of projects funded in the past are available on the program web site, along with details of the grant guidelines.

  10. Metropolitan Edison Company SEF Loans (FirstEnergy Territory...

    Broader source: Energy.gov (indexed) [DOE]

    grid supply; projects involving the development of a sustainable energy technology (e.g., solar panel manufacturing); businesses that use renewable energy in the operation of a...

  11. MPO SAN ANTONIO - BEXAR COUNTY METROPOLITAN PLANNING ORGANIZATION

    Energy Savers [EERE]

    ... agricultural tractors, combines, commercial airports, point sources, and heavy duty truck idling 2012 With Eagle Ford Emission Inventory WRF v3.2 CAMx 5.40 Local 2012 San ...

  12. Chicago Metropolitan Agency for Planning Summary of Reported...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Retrofit Rebate Program (Pilot) * Multifamily Residential Programs o Community Investment Corporation (CIC) Energy Savers Multifamily Loan Program o Village of Oak Park...

  13. DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project

    Broader source: Energy.gov [DOE]

    2011 DOE Hydrogen and Fuel Cells Program, and Vehicle Technologies Program Annual Merit Review and Peer Evaluation

  14. Washington Metropolitan Area Transit Authority: Biodiesel Fuel Comparison Final Data Report

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

  15. Emission Testing of Washington Metropolitan Area Transit Authority (WMATA) Natural Gas and Diesel Transit Buses

    SciTech Connect (OSTI)

    Melendez, M.; Taylor, J.; Wayne, W. S.; Smith, D.; Zuboy, J.

    2005-12-01

    An evaluation of emissions of natural gas and diesel buses operated by the Washington Metro Area Transit Authority.

  16. DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project

    Broader source: Energy.gov [DOE]

    2012 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  17. DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project

    Broader source: Energy.gov [DOE]

    2010 DOE Vehicle Technologies and Hydrogen Programs Annual Merit Review and Peer Evaluation Meeting, June 7-11, 2010 -- Washington D.C.

  18. A Blueprint for Urban Sustainability: Integrating Sustainable Energy Practices into Metropolitan Planning, May 2004

    Office of Energy Efficiency and Renewable Energy (EERE)

    Blueprint to define sustainable energy planning as integrated energy & environmental planning to support community sustainability

  19. Materials Data on TiH12(NF2)4 (SG:56) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. Results of radiological measurements taken in the Niagara Falls, New York, area (NF002)

    SciTech Connect (OSTI)

    Williams, J.K.; Berven, B.A.

    1986-11-01

    The results of a radiological survey of 100 elevated gamma radiation anomalies in the Niagara Falls, New York, area are presented. These radiation anomalies were identified by a mobile gamma scanning survey during the period October 3-16, 1984, and were recommended for an onsite survey to determine if the elevated levels of radiation may be related to the transportation of radioactive waste material to the Lake Ontario Ordnance Works for storage. In this survey, radiological measurements included outdoor gamma exposure rates at 1 m above the surface; outdoor gamma exposure rates at the surface, range of gamma exposure rates during scan; and uranium, radium, and thorium concentrations in biased surface soil samples. The results show 38 anomalies (35 located along Pletcher Road and 3 associated with other unreleated locations) were found to exceed Formerly Utilized Sites Remedial Action Program (FUSRAP) remedial action guidelines and were recommended for formal characterization surveys. (Since the time of this survey, remedial actions have been conducted on the 38 anomalies identified as exceeding FUSRAP guidelines, and the radioactive material above guidelines has been removed.) The remaining 62 anomalies are associated with asphalt driveways and parking lots, which used a phosphate slag material (previously identified as cyclowollastonite, synthetic CaSiO/sub 3/). This rocky-slag waste material was used for bedding under asphalt surfaces and in general gravel applications. Most of the contaminated soil and rock samples collected at the latter anomalies had approximately equal concentrations of /sup 226/Ra and /sup 238/U and, therefore, are not related to materials connected with the Niagara Falls Storage Site (NFSS), including material that was transported to the NFSS. 13 refs., 7 figs., 14 tabs.

  1. Materials Data on Ca2NF (SG:141) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Materials Data on Pr3NF6 (SG:1) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Materials Data on H5NF2 (SG:53) by Materials Project

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Suppression of NF-κB signaling and NLRP3 inflammasome activation...

    Office of Scientific and Technical Information (OSTI)

    ... Resource Type: Journal Article Resource Relation: Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 281; Journal Issue: 1; Other Information: Copyright (c) 2014 ...

  5. Nucleon, $$\\Delta$$ and $$\\Omega$$ excited states in $N_f=2+1$ lattice QCD

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    John Bulava; Edwards, Robert G.; Engelson, Eric; Joo, Balint; Lin, Huey -Wen; Morningstar, Colin; Richards, David G.; Wallace, Stephen J.

    2010-07-22

    The energies of the excited states of the Nucleon,more » $$\\Delta$$ and $$\\Omega$$ are computed in lattice QCD, using two light quarks and one strange quark on anisotropic lattices. The calculation is performed at three values of the light quark mass, corresponding to pion masses $$m_{\\pi}$$ = 392(4), 438(3) and 521(3) MeV. We employ the variational method with a large basis of interpolating operators enabling six energies in each irreducible representation of the lattice to be distinguished clearly. We compare our calculation with the low-lying experimental spectrum, with which we find reasonable agreement in the pattern of states. In addition, the need to include operators that couple to the expected multi-hadron states in the spectrum is clearly identified.« less

  6. Final report for "Characterization of Fine Particulate Matter (PM) and secondary PM Precursor Gases in the Mexico City Metropolitan Area"

    SciTech Connect (OSTI)

    Prof. Jose-Luis Jimenez

    2009-05-18

    The objectives of this funded project were (a) to further analyze the data collected by our group and collaborators in Mexico City during the MCMA-2003 field campaign, with the goal of further our understanding of aerosol sources and processes; and (b) to deploy several advanced instruments, including the newly developed high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS) and thermal-denuder (TD), during the MILAGRO/MAX-Mex/MCMA-2006 field campaign, and to analyze those data (together with the 2003 data) to provide additional insights on the formation and transformation of aerosols in the Mexico City area. These goals were addressed in collaboration with our project partners, MIT/Molina Center, and Aerodyne Research. Overall this project was very successful, resulting on 22+ journal papers including six highly cited papers and three papers that are the most cited in their respective journals (out of several thousand papers) since the year in which they were published. Multiple discoveries, such as the the underestimation of SOA in urban areas even for short photochemical ages, the demonstration that urban POA is of similar or higher volatility than urban SOA, and the first analysis of organic aerosol elemental composition in real-time have been recently published. Several dozen presentations at major US and international conferences and seminars also acknowledged this grant.

  7. Case history of implementation of conservation program in a multitude of diverse buildings in the metropolitan area

    SciTech Connect (OSTI)

    Khan, S.M.

    1982-06-01

    This paper outlines an energy conservation program undertaken by Jazco Corporation. Monitoring techniques were based on a calculated energy norm. Abnormalities, internal heat load, and switch-over temperature were also established. An actual physical audit verified the results. HVAC systems were found to be incompatible. Most boilers were derated. An electronic economizer cycle was installed. Occupied temperature setting, night temperature setback, dynamic load control, demand control, were all instrumented with savings. Microprocessor-based systems replaced main frame computers at a fraction of the cost. It was found that New York state lighting standards are good except where frequency of use is low.

  8. Onondaga County Department of Water Environment Protection: Process Optimization Saves Energy at Metropolitan Syracuse Wastewater Treatment Plant

    SciTech Connect (OSTI)

    Not Available

    2005-12-01

    This DOE Industrial Technologies Program spotlight describes how Onondaga County, New York, is saving nearly 3 million kWh and 270 million Btu annually at a wastewater treatment plant after replacing inefficient motors and upgrading pumps.

  9. EV Community Readiness projects: Delaware Valley Regional Planning Commission (PA); Metropolitan Energy Information Center, Inc. (KS, MO)

    Broader source: Energy.gov [DOE]

    2013 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Program Annual Merit Review and Peer Evaluation Meeting

  10. Onondaga County Department of Water Environment Protection: Process Optimization Saves Energy at Metropolitan Syracuse Wastewater Treatment Plant

    SciTech Connect (OSTI)

    2010-06-25

    This DOE Industrial Technologies Program spotlight describes how Onondaga County, New York, is saving nearly 3 million kWh and 270 million Btu annually at a wastewater treatment plant after replacing inefficient motors and upgrading pumps.

  11. Property:SurfaceManager | Open Energy Information

    Open Energy Info (EERE)

    + COCONINO NF + AZA-009169 + COCONINO NF + AZA-009170 + COCONINO NF + AZA-009171 + FOREST SERVICE + AZA-009172 + FOREST SERVICE + AZA-009173 + FOREST SERVICE + AZA-009174 +...

  12. Pioglitazone inhibits angiotensin II-induced atrial fibroblasts proliferation via NF-?B/TGF-?1/TRIF/TRAF6 pathway

    SciTech Connect (OSTI)

    Chen, Xiao-qing; Liu, Xu; Wang, Quan-xing; Zhang, Ming-jian; Guo, Meng; Liu, Fang; Jiang, Wei-feng; Zhou, Li

    2015-01-01

    The exact mechanisms underlying inhibitory effects of pioglitazone (Pio) on Angiotensin II (AngII)-induced atrial fibrosis are complex and remain largely unknown. In the present study, we examined the effect of Pio on AngII-induced mice atrial fibrosis in vivo and atrial fibroblasts proliferation in vitro. In vivo study showed that AngII infusion induced atrial fibrosis and increased expressions of Toll/IL-1 receptor domain-containing adaptor inducing IFN-? (TRIF) and tumor necrosis factor receptor associated factor 6 (TRAF6) in mice models. However, those effects could be attenuated by Pio (P<0.01). As for in vitro experiment, Pio suppressed AngII-induced atrial fibroblasts proliferation via nuclear factor-?B/transforming growth factor-?1/TRIF/TRAF6 signaling pathway in primary cultured mice atrial fibroblasts (P<0.01). In conclusion, suppression of Pio on AngII-induced atrial fibrosis might be related to its inhibitory effects on above signaling pathway. - Highlights: Angiotensin II increased atrial fibrosis and related gene expressions in mice. Angiotensin II induced atrial fibroblasts proliferation by activating signaling pathway. Pioglitazone reversed both aforementioned changes.

  13. DOE/EIA-0555(94)71

    U.S. Energy Information Administration (EIA) Indexed Site

    g The most recent definitions of Metropolitan Statistical Areas--issued by the Office of Management and Budget formerly known as Standard Metropolitan Statistical Areas were those...

  14. 1992 CBECS C & E

    U.S. Energy Information Administration (EIA) Indexed Site

    of District Heat by End Use, 1989 District Heat Consumption (trillion Btu) Space Water a Total Heating Heating Other RSE Building Row Characteristics Factor 1.0 NF NF NF RSE...

  15. 1992 CBECS C & E

    U.S. Energy Information Administration (EIA) Indexed Site

    0. Consumption of Fuel Oil by End Use, 1989 Fuel Oil Consumption (trillion Btu) Space Water a Total Heating Heating Other RSE Building Row Characteristics Factor 1.0 NF NF NF RSE...

  16. FirstEnergy (MetEdison, Penelec, Penn Power, West Penn Power...

    Broader source: Energy.gov (indexed) [DOE]

    Honeywell Website http:www.firstenergycorp.comenergyefficiencypennsylvaniaindex.html Funding Source Pennsylvania Electric Company (Penelec), Metropolitan Edison Company...

  17. Better Buildings Residential Network Peer Exchange Call Series...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Metropolitan Energy Center Nidec Motor Corporation Philadelphia Gas Works Smith & Boucher Engineers Snohomish County PUD South Bay Cities Council of ...

  18. Effect of Sea Level Rise on Energy Infrastructure in Four Major

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Metropolitan Areas (September 2014) | Department of Energy Effect of Sea Level Rise on Energy Infrastructure in Four Major Metropolitan Areas (September 2014) Effect of Sea Level Rise on Energy Infrastructure in Four Major Metropolitan Areas (September 2014) The Office of Electricity Delivery and Energy Reliability has released findings of a pilot study that explores the feasibility of assessing the impacts of sea level rise on energy infrastructure. The goal of the study was to develop a

  19. Electronic DOE Information Security System (eDISS) PIA

    Energy Savers [EERE]

    Effect of Sea Level Rise on Energy Infrastructure in Four Major Metropolitan Areas September 2014 U.S. Department of Energy Office of Electricity Delivery and Energy Reliability Pilot Study on the Effect of Sea Level Rise on Energy Infrastructure in Four Major Metropolitan Areas August 2014 Effect of Sea Level Rise on Energy Infrastructure in Four Major Metropolitan Areas Office of Electricity Delivery and Energy Reliability U.S. Department of Energy September 2014 i Table of Contents 1.

  20. AVTA: Chargepoint America | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Chargepoint America AVTA: Chargepoint America ChargePoint America deployed 4,600 public and private charging stations in 9 metropolitan areas. ChargePoint America Recovery Act ...

  1. University of Toledo | Open Energy Information

    Open Energy Info (EERE)

    Toledo Jump to: navigation, search Name: University of Toledo Place: Toledo, Ohio Zip: 43606-3390 Product: A student-centered public metropolitan research university. Coordinates:...

  2. Vehicle Technologies Office Merit Review 2016: Safe Alternative Fuels Deployment in Mid-America (The SAFD Project)

    Office of Energy Efficiency and Renewable Energy (EERE)

    Presentation given by Metropolitan Energy Center, Inc. at the 2016 DOE Vehicle Technologies Office and Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting about...

  3. South West Solar Power | Open Energy Information

    Open Energy Info (EERE)

    Power Jump to: navigation, search Name: South West Solar Power Place: Gwangju Metropolitan City, Korea (Republic) Product: Korea-based company acting as the operating body for the...

  4. Microsoft PowerPoint - Meyer Day 2.ppt

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Critical Metropolitan NY - Mid-Atlantic coastal area Southern California Concern New England Seattle-Portland SF Bay Area Phoenix-Tucson Conditional Montana-Wyoming ...

  5. Tax Credits, Rebates & Savings | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Metropolitan Edison Company SEF Loans (FirstEnergy Territory) The fund is designed to promote: Eligibility: Commercial, Industrial, Local Government, Nonprofit, Schools Savings...

  6. Clean Cities: Greater New Haven Clean Cities coalition

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    alternative vehicle and fuel consultation and assistance to many organizations in Connecticut and outside the state, including several towns and cities, metropolitan transit...

  7. FirstEnergy (MetEdison, Penelec, Penn Power, West Penn Power)- Residential Energy Efficiency Programs

    Office of Energy Efficiency and Renewable Energy (EERE)

    First Energy as a parent company administers the energy efficiency program for Metropolitan Edition (Met-Ed), Pennsylvania Electric (Penelec), Pennsylvania Power (Penn Power), and West Penn Power. ...

  8. OpenEI:Projects/Geographic Pages/Colorado: Energy Resources ...

    Open Energy Info (EERE)

    the mountains offer hydroelectric power possibilities. Within more metropolitan areas, solar has been gaining momentum as more private sector activity has been driven by...

  9. Alternative Fuels Data Center

    Alternative Fuels and Advanced Vehicles Data Center [Office of Energy Efficiency and Renewable Energy (EERE)]

    Compressed Natural Gas (CNG) Vehicle Incentives - Metropolitan Utilities District (MUD) ... by the MUD are eligible for a 500 rebate for the purchase of a dedicated CNG vehicle. ...

  10. SRI2007 Conference - Travel Tips

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Getting to the Hilton Baton Rouge Capitol Center: From the Baton Rouge Metropolitan Airport (BTR) From the New Orleans International Airport (MSY) Shuttles to and from the Baton...

  11. A Blueprint for Urban Sustainability: Integrating Sustainable...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    A Blueprint for Urban Sustainability: Integrating Sustainable Energy Practices into Metropolitan Planning, May 2004 A Blueprint for Urban Sustainability: Integrating Sustainable ...

  12. EIS-0417: Final Environmental Impact Statement | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EIS-0417: Final Environmental Impact Statement South Mountain Freeway (Loop 202); Phoenix, ... impacts of a proposed new multi-lane freeway in the metropolitan Phoenix area. ...

  13. EIS-0417: Record of Decision | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EIS-0417: Record of Decision South Mountain Freeway (Loop 202); Phoenix, Arizona Federal ... South Mountain Freeway (Loop 202) project in the Greater Metropolitan Phoenix Area. ...

  14. COMPNAME","COMPID","YEAR","PLANTNAME","KIND","CONSTRUC","INSTALLED...

    U.S. Energy Information Administration (EIA) Indexed Site

    RESERV","EQUIP","ROADS","TOTCOST","COSTCAP","GROSSEXP","OPERENG","WATER","HYDRAULIC","ELEC...,75598,116503,3632,844883,2 "Metropolitan Water District",12397,1999,"VENICE","Closed ...

  15. Eight Approaches to Enable Greater Energy Efficiency: A Guide...

    Broader source: Energy.gov (indexed) [DOE]

    State Legislatures (NCSL) is the bipartisan organization air pollution control agencies in 53 states and territories and over 165 major metropolitan areas across the United States. ...

  16. Transmission Siting_071508.indd

    Broader source: Energy.gov (indexed) [DOE]

    ... The National Association of Clean Air Agencies (NACAA) represents air pollution control agencies in 53 states and territories and over 165 major metropolitan areas across the ...

  17. S:\VM3\RX97\TBL_LIST.WPD

    U.S. Energy Information Administration (EIA) Indexed Site

    Million U.S. Households, 1997 Housing Unit Characteristics RSE Column Factor: Total Four Most Populated States RSE Row Factors New York California Texas Florida 0.4 1.1 1.1 1.2 1.7 Total .............................................................. 101.5 6.8 11.5 7.0 5.9 NF Census Region and Division Northeast ..................................................... 19.7 6.8 -- -- -- NF New England ............................................. 5.3 Q -- -- -- NF Middle Atlantic

  18. Evolutionary analyses of non-family genes in plants

    SciTech Connect (OSTI)

    Ye, Chuyu; Li, Ting; Yin, Hengfu; Weston, David; Tuskan, Gerald A; Tschaplinski, Timothy J; Yang, Xiaohan

    2013-03-01

    There are a large number of non-family (NF) genes that do not cluster into families with three or more members per genome. While gene families have been extensively studied, a systematic analysis of NF genes has not been reported. We performed comparative studies on NF genes in 14 plant species. Based on the clustering of protein sequences, we identified ~94,000 NF genes across these species that were divided into five evolutionary groups: Viridiplantae-wide, angiosperm-specific, monocot-specific, dicot-specific, and those that were species-specific. Our analysis revealed that the NF genes resulted largely from less frequent gene duplications and/or a higher rate of gene loss after segmental duplication relative to genes in both low-copy-number families (LF; 3 10 copies per genome) and high-copy-number families (HF; >10 copies). Furthermore, we identified functions enriched in the NF gene set as compared with the HF genes. We found that NF genes were involved in essential biological processes shared by all plant lineages (e.g., photosynthesis and translation), as well as gene regulation and stress responses associated with phylogenetic diversification. In particular, our analysis of an Arabidopsis protein-protein interaction network revealed that hub proteins with the top 10% most connections were over-represented in the NF set relative to the HF set. This research highlights the roles that NF genes may play in evolutionary and functional genomics research.

  19. Evolutionary analyses of non-family genes in plants

    SciTech Connect (OSTI)

    Ye, Chuyu; Li, Ting; Yin, Hengfu; Weston, David; Tuskan, Gerald A; Tschaplinski, Timothy J; Yang, Xiaohan

    2013-01-01

    There are a large number of non-family (NF) genes that do not cluster into families with three or more members per genome. While gene families have been extensively studied, a systematic analysis of NF genes has not been reported. We performed comparative studies on NF genes in 14 plant species. Based on the clustering of protein sequences, we identified ~94 000 NF genes across these species that were divided into five evolutionary groups: Viridiplantae wide, angiosperm specific, monocot specific, dicot specific, and those that were species specific. Our analysis revealed that the NF genes resulted largely from less frequent gene duplications and/or a higher rate of gene loss after segmental duplication relative to genes in both lowcopy- number families (LF; 3 10 copies per genome) and high-copy-number families (HF; >10 copies). Furthermore, we identified functions enriched in the NF gene set as compared with the HF genes. We found that NF genes were involved in essential biological processes shared by all plant lineages (e.g. photosynthesis and translation), as well as gene regulation and stress responses associated with phylogenetic diversification. In particular, our analysis of an Arabidopsis protein protein interaction network revealed that hub proteins with the top 10% most connections were over-represented in the NF set relative to the HF set. This research highlights the roles that NF genes may play in evolutionary and functional genomics research.

  20. NVN-079292 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1980.23 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  1. UTU-085605 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSECOMPPOST2005 Total Acreage 2441.53 Bid PriceAcre Managing Field Office none provided Surface Manager FISHLAKE NF Lessee 1 ENEL...

  2. EIS-0350-S1: Mitigation Action Plan | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Los Alamos, NM This Mitigation Action Plan (MAP) describes mitigation and monitoring commitments for constructing and operating the Modified CMRR-NF. The commitments made in this ...

  3. USFS Humboldt-Toiyabe National Forest | Open Energy Information

    Open Energy Info (EERE)

    USFS Humboldt-Toiyabe National Forest Jump to: navigation, search Name: USFS Humboldt-Toiyabe National Forest Abbreviation: Humbolt-Toiyabe NF Address: 1200 Franklin Way Place:...

  4. NVN-074253 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1280 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  5. NVN-074250 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1199 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  6. NVN-077646 | Open Energy Information

    Open Energy Info (EERE)

    Acres) Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 80 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  7. NVN-079352 | Open Energy Information

    Open Energy Info (EERE)

    Acres) Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1160 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  8. NVN-074254 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 2280 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  9. NVN-079310 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 655 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  10. NVN-079667 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1920 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  11. NVN-079664 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1280 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  12. NVN-079276 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 640 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  13. Carbon Free Developments | Open Energy Information

    Open Energy Info (EERE)

    Free Developments Jump to: navigation, search Name: Carbon Free Developments Place: United Kingdom Zip: W11 3NF Sector: Wind energy Product: UK-based onshore wind project...

  14. Effect of Sea Level Rise on Energy Infrastructure in Four Major...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The study focused on the four major metropolitan statistical areas of New York City, Houston, Miami, and Los Angeles. These areas were chosen because of their proximity to the ...

  15. EIA Energy Information Administration

    U.S. Energy Information Administration (EIA) Indexed Site

    metropolitan areas (Baltimore, New York, Philadelphia, Washington, DC and others), a &24;Red Alert&23; that signifies dangerous air quality was in effect for 2 to 3 days and local...

  16. Tax Credits, Rebates & Savings | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    Metropolitan Edison Company SEF Grants (FirstEnergy Territory) Examples of projects funded in the past are available on the program web site, along with details of the grant...

  17. CX-009364: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project CX(s) Applied: A1, B5.1 Date: 09/19/2012 Location(s): Georgia Offices(s): National Energy Technology Laboratory

  18. CX-007595: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project CX(s) Applied: A1 Date: 01/26/2012 Location(s): Georgia Offices(s): National Energy Technology Laboratory

  19. CX-007592: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project CX(s) Applied: B5.22 Date: 01/27/2012 Location(s): Georgia Offices(s): National Energy Technology Laboratory

  20. QGESS: Specification for Selected Feedstocks

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in Exhibit 1-1, which is based on the mean of over 6,800 samples of pipeline quality natural gas taken in 26 major metropolitan areas of the United States (U.S.). 1 Exhibit 1-1...

  1. CX-004443: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    State Energy Program - Combined Heat and Power - Metropolitan CouncilCX(s) Applied: B5.1Date: 11/16/2010Location(s): MinnesotaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  2. Fact #727: May 14, 2012 Nearly Twenty Percent of Households Own...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Trends in the United States and its Major Metropolitan Area, 1960-1990, Cambridge, MA, 1994, p. 2-2. 2000 data - U.S. Bureau of the Census, American Fact Finder, ...

  3. Distribution Category UC-98 Consumption End-Use A Comparison...

    U.S. Energy Information Administration (EIA) Indexed Site

    buildings) as well as a list of large buildings in each metropolitan area. MECS is based upon a comprehensive list of manufactures that is maintained by the Census Bureau for...

  4. Washington, D.C. - Local Information | NREL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and economical way to travel to NREL and within the metropolitan area. The L'Enfant Plaza metro station is the closest station to NREL. There are multiple exits at the L'Enfant...

  5. Vehicle Technologies Office Merit Review 2014: Mid-America Collaborative for Alternative Fuels Implementation

    Broader source: Energy.gov [DOE]

    Presentation given by Metropolitan Energy Center, Inc. at 2014 DOE Hydrogen and Fuel Cells Program and Vehicle Technologies Office Annual Merit Review and Peer Evaluation Meeting about Mid-America...

  6. --No Title--

    U.S. Energy Information Administration (EIA) Indexed Site

    CENDIV. Metropolitan statistical area MSA4 11- 11 MSA. Climate zone CLIMATE4 13- 13 CLIMAT. B1 Square footage SQFT4 15- 22 MISS8CH. B2 Square footage SQFTC4 24- 25 SQFTC. ...

  7. Airports & Lodging | Savannah River Ecology Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Columbia, SC Columbia Metropolitan Airport - all major carriers; 1.5-2h drive to SREL. Atlanta, GA Hartsfield Airport - all major carriers; 2.5-3 hour drive from Atlanta, GA, to ...

  8. Urban Surfaces and Heat Island Mitigation Potentials (Journal...

    Office of Scientific and Technical Information (OSTI)

    areas of Chicago, IL, Houston, TX, Sacramento, CA, and Salt Lake City, UT. The digital high resolution (0.3 to 0.5-m) aerial photographs for each of these metropolitan areas ...

  9. QER Public Meeting in Denver, CO: Gas-Electricity Interdependencies...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    July, 28 2014 - 9:00 AM MDT Metropolitan State University of Denver Auraria Campus - St. Cajetan's Center 1190 9th Street Denver, CO 80204 Click Here to view the live stream of ...

  10. DOE commits more than $1.7 million to help commercialize promising

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Argonne-associated energy technologies | Argonne National Laboratory Argonne and the Metropolitan Water Reclamation District of Greater Chicago will be working on a more efficient and cost-effective method for removing trace organic contaminants in our water supply. (M. Szczygiel/Shutterstock) Argonne and the Metropolitan Water Reclamation District of Greater Chicago will be working on a more efficient and cost-effective method for removing trace organic contaminants in our water supply. (M.

  11. Empirical Evaluation of Four Microwave Radiative Forward Models Based on Ground-Based Radiometer Data Near 20 and 30 GHz

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Emission Testing of Washington Metropolitan Area Transit Authority (WMATA) Natural Gas and Diesel Transit Buses M. Melendez, J. Taylor, and J. Zuboy National Renewable Energy Laboratory W.S. Wayne West Virginia University D. Smith U.S. Department of Energy Technical Report NREL/TP-540-36355 December 2005 Emission Testing of Washington Metropolitan Area Transit Authority (WMATA) Natural Gas and Diesel Transit Buses M. Melendez, J. Taylor, and J. Zuboy National Renewable Energy Laboratory W.S.

  12. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050b

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    2015-01-01

    MILC asqtad QCD SU(3) gauge ensemble; series=b; a=0.11fm minus 0.0022fm; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  13. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    2015-01-01

    MILC asqtad QCD SU(3) gauge ensemble; series=a; a=0.11fm minus 0.0022fm ; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  14. Search for: All records | SciTech Connect

    Office of Scientific and Technical Information (OSTI)

    ... Lam, Amy Y. (1) Lamothe, Betty (1) Lin, Su-Chang (1) Lo, Yu-Chih (1) Min, Tongpil (1) ... of the NF-B Inhibitor A20 Lin, Su-Chang ; Chung, Jee Y. ; Lamothe, Betty ; ...

  15. NVN-080159 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 NGP BLUE MOUNTAIN I LLC Lessee 2 none provided Effective Date 7312016 Expire Date Held By...

  16. CACA-014407 | Open Energy Information

    Open Energy Info (EERE)

    Acres) Case Status Authorized Case Type GEOLSECOMPPRE2005 Total Acreage 2561.25 Bid PriceAcre Managing Field Office none provided Surface Manager INYO NF Lessee 1 ORNI 51 LLC...

  17. NVN-079105 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 2356.36 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 ORNI 16...

  18. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    MILC asqtad QCD SU(3) gauge ensemble; series=a; a=0.11fm minus 0.0022fm ; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  19. Lattice QCD gauge ensemble: USQCD/MILC/asqtad/2064f21b678m010m050b

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Aubin, Christopher Alan [Fordham U.; Bernard, Claude W. [Washington U. St. Louis; Burch, Tommy [U. Regensburg; Datta, Saumen [Tata Institute; DeGrand, Thomas Alan [Colorado U., Boulder; DeTar, Carleton E. [Utah U.; Gottlieb, Steven A. [Indiana U., Bloomington; Gregory, Eric Brittain [Wuppertal U.; Heller, Urs M. [American Physical Society; Hetrick, James Edward [U. Pacific, Stockton; Orginos, Kostas Nikolaou [William-Mary Coll.; Osborn, James C. [Argonne National Laboratory, ALCF; Toussaint, W. Doug [Arizona U.; Sugar, Robert L. [U. C., Santa Barbara

    MILC asqtad QCD SU(3) gauge ensemble; series=b; a=0.11fm minus 0.0022fm; Ls=2.16fm; Nf=2+1; u0.m0=(0.010,0.050)

  20. AZA-009191 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT OIL USA INC Lessee 2 none provided Effective Date Expire Date Held By Production (HBP) HBP...

  1. AZA-009190 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT OIL USA INC Lessee 2 none provided Effective Date Expire Date Held By Production (HBP) HBP...

  2. AZA-009186 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT OIL USA INC Lessee 2 none provided Effective Date Expire Date Held By Production (HBP) HBP...

  3. Subject:

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    rotation reversals, poloidal impurity asymmetries and "non-local" heat transport Gao thesis 2015, Rice NF 2013). We are not yet certain if our RF L-mode target plasmas will...

  4. 1992 CBECS C & E

    U.S. Energy Information Administration (EIA) Indexed Site

    of Natural Gas by End Use, 1989 Natural Gas Consumption (trillion Btu) Space Water a Total Heating Heating Cooking Other RSE Building Row Characteristics Factor 1.0 NF...

  5. Systems and methods for treating material

    DOE Patents [OSTI]

    Scheele, Randall D; McNamara, Bruce K

    2014-10-21

    Systems for treating material are provided that can include a vessel defining a volume, at least one conduit coupled to the vessel and in fluid communication with the vessel, material within the vessel, and NF.sub.3 material within the conduit. Methods for fluorinating material are provided that can include exposing the material to NF.sub.3 to fluorinate at least a portion of the material. Methods for separating components of material are also provided that can include exposing the material to NF.sub.3 to at least partially fluorinate a portion of the material, and separating at least one fluorinated component of the fluorinated portion from the material. The materials exposed to the NF.sub.3 material can include but are not limited to one or more of U, Ru, Rh, Mo, Tc, Np, Pu, Sb, Ag, Am, Sn, Zr, Cs, Th, and/or Rb.

  6. CACA-011667 | Open Energy Information

    Open Energy Info (EERE)

    Surface Manager INYO NF Lessee 1 MAMMOTH-PACIFIC LP Lessee 2 none provided Effective Date 311982 Expire Date Held By Production (HBP) HELD BY PROD-Actual HBP Date 12221990...

  7. NVN-079291 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 612006 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  8. NVN-079666 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 712005 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  9. NVN-078688 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 REESE RIVER GEO POWER LLC Lessee 2 none provided Effective Date 1012004 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  10. NVN-079665 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 712005 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  11. NVN-079307 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 712006 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  12. NVN-079106 | Open Energy Information

    Open Energy Info (EERE)

    Surface Manager TOIYABE NF Lessee 1 ORNI 16 LLC Lessee 2 none provided Effective Date 912006 Expire Date Held By Production (HBP) HBP Date RoyaltyRate 10% - 5% - 5% Most...

  13. NVN-077647 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 612006 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  14. NVN-079662 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 6302015 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  15. NVN-074249 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 912007 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  16. NVN-079663 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 712005 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  17. CACA-011672 | Open Energy Information

    Open Energy Info (EERE)

    provided Surface Manager INYO NF Lessee 1 ORNI 10 LLC Lessee 2 none provided Effective Date 111982 Expire Date Held By Production (HBP) HELD BY PROD-Actual HBP Date 12311998...

  18. NVN-078687 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 REESE RIVER GEO POWER LLC Lessee 2 none provided Effective Date 1012004 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  19. NVN-079668 | Open Energy Information

    Open Energy Info (EERE)

    Manager TOIYABE NF Lessee 1 GRADIENT RESOURCES INC Lessee 2 none provided Effective Date 712005 Expire Date Held By Production (HBP) HBP Date RoyaltyRate Most Recent Action...

  20. CACA-014408 | Open Energy Information

    Open Energy Info (EERE)

    Surface Manager INYO NF Lessee 1 MAMMOTH-PACIFIC LP Lessee 2 none provided Effective Date 7191983 Expire Date Held By Production (HBP) HELD BY PROD-Actual HBP Date 782006...

  1. YEAR

    National Nuclear Security Administration (NNSA)

    300 YEAR 2011 Males 109 Females 191 YEAR 2011 SES 9 EJEK 1 NN (Engineering) 2 NQ (ProfTechAdmin) 203 NU (TechAdmin Support) 38 NF (Future Ldrs) 47 YEAR 2011 American Indian...

  2. YEAR

    National Nuclear Security Administration (NNSA)

    86 YEAR 2012 Males 103 Females 183 YEAR 2012 SES 7 EJEK 1 NN (Engineering) 1 NQ (ProfTechAdmin) 202 NU (TechAdmin Support) 30 NF (Future Ldrs) 45 YEAR 2012 American Indian Male...

  3. Bioremediation of contaminated groundwater

    DOE Patents [OSTI]

    Hazen, T.C.; Fliermans, C.B.

    1994-01-01

    Disclosed is an apparatus and method for in situ remediation of contaminated subsurface soil or groundwater contaminated by chlorinated hydrocarbons. A nutrient fluid (NF) is selected to simulated the growth and reproduction of indigenous subsurface microorganisms capable of degrading the contaminants; an oxygenated fluid (OF) is selected to create an aerobic environment with anaerobic pockets. NF is injected periodically while OF is injected continuously and both are extracted so that both are drawn across the plume. NF stimulates microbial colony growth; withholding it periodically forces the larger, healthy colony of microbes to degrade the contaminants. Treatment is continued until the subsurface concentration of contaminants is acceptable. NF can be methane and OF be air, for stimulating production of methanotrophs to break down chlorohydrocarbons, especially TCE and tetrachloroethylene.

  4. Nucleophilic fluorination of aromatic compounds

    DOE Patents [OSTI]

    Satyamurthy, Nagichettiar; Barrio, Jorge R

    2014-03-18

    Iodylbenzene derivatives substituted with electron donating as well as electron withdrawing groups on the aromatic ring are used as precursors in aromatic nucleophilic substitution reactions. The iodyl group (IO.sub.2) is regiospecifically substituted by nucleophilic fluoride to provide the corresponding fluoroaryl derivatives. No-carrier-added [F-18]fluoride ion derived from anhydrous [F-18](F/Kryptofix, [F-18]CsF or a quaternary ammonium fluoride (e.g., Me.sub.4NF, Et.sub.4NF, n-Bu.sub.4NF, (PhCH.sub.2).sub.4NF) exclusively substitutes the iodyl moiety in these derivatives and provides high specific activity F-18 labeled fluoroaryl analogs. Iodyl derivatives of a benzothiazole analog and 6-iodyl-L-dopa derivatives have been synthesized as precursors and have been used in the preparation of no-carrier-added [F-18]fluorobenzothiazole as well as 6-[F-18]fluoro-L-dopa.

  5. AZA-009169 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 2560 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  6. AZA-009170 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 1920 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  7. NVN-077739 | Open Energy Information

    Open Energy Info (EERE)

    Lot Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1271.82 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 PATUA...

  8. AZA-009168 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 2005 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  9. AZA-009175 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 2190 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  10. NVN-079305 | Open Energy Information

    Open Energy Info (EERE)

    1 Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 1811.109 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  11. NVN-079104 | Open Energy Information

    Open Energy Info (EERE)

    Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 2480.99 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 ORNI 16...

  12. NVN-080086 | Open Energy Information

    Open Energy Info (EERE)

    Acres) Case Status Authorized Case Type GEOLEASENONCOMPETITI Total Acreage 650.32 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 NGP BLUE...

  13. NVN-079306 | Open Energy Information

    Open Energy Info (EERE)

    B Case Status Authorized Case Type GEOLSENONCOMPPRE2005 Total Acreage 2140.53 Bid PriceAcre Managing Field Office none provided Surface Manager TOIYABE NF Lessee 1 GRADIENT...

  14. Structural studies of magnesium nitride fluorides by powder neutron diffraction

    SciTech Connect (OSTI)

    Brogan, Michael A.; Hughes, Robert W.; Smith, Ronald I.; Gregory, Duncan H.

    2012-01-15

    Samples of ternary nitride fluorides, Mg{sub 3}NF{sub 3} and Mg{sub 2}NF have been prepared by solid state reaction of Mg{sub 3}N{sub 2} and MgF{sub 2} at 1323-1423 K and investigated by powder X-ray and powder neutron diffraction techniques. Mg{sub 3}NF{sub 3} is cubic (space group: Pm3m) and has a structure related to rock-salt MgO, but with one cation site vacant. Mg{sub 2}NF is tetragonal (space group: I4{sub 1}/amd) and has an anti-LiFeO{sub 2} related structure. Both compounds are essentially ionic and form structures in which nitride and fluoride anions are crystallographically ordered. The nitride fluorides show temperature independent paramagnetic behaviour between 5 and 300 K. - Graphical abstract: Definitive structures of the ternary magnesium nitride fluorides Mg{sub 3}NF{sub 3} and the lower temperature polymorph of Mg{sub 2}NF have been determined from powder neutron diffraction data. The nitride halides are essentially ionic and exhibit weak temperature independent paramagnetic behaviour. Highlights: Black-Right-Pointing-Pointer Definitive structures of Mg{sub 3}NF{sub 3} and Mg{sub 2}NF were determined by neutron diffraction. Black-Right-Pointing-Pointer Nitride and fluoride anions are crystallographically ordered in both structures. Black-Right-Pointing-Pointer Both compounds exhibit weak, temperature independent paramagnetic behaviour. Black-Right-Pointing-Pointer The compounds are essentially ionic with ionicity increasing with F{sup -} content.

  15. Tri-Cities Index of Innovation and Technology

    SciTech Connect (OSTI)

    Fowler, Richard A.; Scott, Michael J.; Butner, Ryan S.

    2011-01-17

    In 2001 and 2004, the Economic Development Office of Pacific Northwest National Laboratory published companion reports to the Washington Technology Center Index studies that provided additional information on the Tri-Cities (Kennewick-Richland-Pasco) area of the state, its technology businesses, and important advantages that the Tri-Cities have as places to live and do business. These reports also compared the Tri-Cities area to other technology-based metropolitan areas in the Pacific Northwest and nation along critical dimensions known to be important to technology firms. This report updates the material in these earlier reports, and highlights a growing Tri-Cities metropolitan area.

  16. Enact legislation supporting residential property assessed clean energy financing (PACE)

    SciTech Connect (OSTI)

    Saha, Devashree

    2012-11-15

    Congress should enact legislation that supports residential property assessed clean energy (PACE) programs in the nation’s states and metropolitan areas. Such legislation should require the Federal Housing Finance Agency (FHFA) to allow Fannie Mae and Freddie Mac to purchase residential mortgages with PACE assessments while at the same time providing responsible underwriting standards and a set of benchmarks for residential PACE assessments in order to minimize financial risks to mortgage holders. Congressional support of residential PACE financing will improve energy efficiency, encourage job creation, and foster economic growth in the nation’s state and metropolitan areas.

  17. Areas Participating in the Oxygenated Gasoline Program (Released in the STEO July 1999)

    Reports and Publications (EIA)

    1999-01-01

    Section 211(m) of the Clean Air Act (42 U.S.C. 7401-7671q) requires that gasoline containing at least 2.7% oxygen by weight is to be used in the wintertime in those areas of the county that exceed the carbon monoxide National Ambient Air Quality Standards (NAAQS). The winter oxygenated gasoline program applies to all gasoline sold in the larger of the Consolidated Metropolitan Statistical Area (CMSA) or Metropolitan Statistical Area (MSA) in which the nonattainment area is located.

  18. Comparative evaluations of the weldability of modified 800H and other advanced austenitic stainless steels

    SciTech Connect (OSTI)

    Lundin, C.D.; Qiao, C.Y.P.

    1993-07-01

    The weldability of modified 800H was evaluated in terms of HAZ hot cracking susceptibility and HAZ softening tendency. Four other types of austenitic alloys, modified 316, 310Ta, HR3C and NF709, were employed to carry out a comparative study. It was found that modified 800H exhibits good weldability in terms of operability as contrasted to NF709. However, a higher HAZ hot cracking susceptibility for the modified 800H tubing heats was revealed as compared to the commercial tubing heats of HR3C and NF709. The hot cracking test results for the small laboratory prepared modified 800H heats showed an equivalent or better HAZ hot cracking resistance as compared to HR3C and NF709. Thus, it is anticipated that modified 800H tubing can show a hot cracking resistance equivalent to NF709 and HR3C if the base metal grain size is properly controlled and an optimum thermal mechanical treatment has been performed. The preliminary assessment on HAZ softening behavior for modified 800H, modified 316, 310Ta, HR3C and NF709 alloys is discussed.

  19. CX-005666: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle ProjectCX(s) Applied: A1, B5.1Date: 04/29/2011Location(s): Marrow, GeorgiaOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  20. CX-001608: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Energy Efficiency and Conservation Block Grant City of Jacksonville: 6) Metropolitan Government Clean Transportation ProgramCX(s) Applied: B5.1Date: 04/07/2010Location(s): Jackson, FloridaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  1. CX-008274: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    DeKalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project CX(s) Applied: B3.6, B5.22 Date: 05/10/2012 Location(s): Georgia Offices(s): National Energy Technology Laboratory

  2. EIS-0417: South Mountain Freeway (Loop 202); Phoenix, Arizona

    Broader source: Energy.gov [DOE]

    Federal Highway Administration and Arizona Department of Transportation, with Western Area Power Administration as a cooperating agency, prepared an EIS that analyzes the potential environmental impacts of the proposed South Mountain Freeway (Loop 202) project in the Greater Metropolitan Phoenix Area.

  3. CX-001791: Categorical Exclusion Determination

    Broader source: Energy.gov [DOE]

    Dekalb County/Metropolitan Atlanta Alternative Fuel and Advanced Technology Vehicle Project (Atlanta)CX(s) Applied: A1, A7Date: 04/21/2010Location(s): Atlanta, GeorgiaOffice(s): Energy Efficiency and Renewable Energy, National Energy Technology Laboratory

  4. Occupant Emergency Plans

    Broader source: Energy.gov [DOE]

    The collection of Emergency Procedures documents for the Department of Energy, Headquarters buildings, in the Washington, DC, metropolitan area. All of these documents are only available to computers attached to the DOE Network. They are for use only by DOE Headquarters employees. • Building Diagrams and Assembly Areas • Occupant Emergency Plans (OEP's)

  5. Energize New York Data Dashboard | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Data Dashboard Energize New York Data Dashboard The data dashboard for Energize New York, a partner in the U.S. Department of Energy's Better Buildings Neighborhood Program. Energize New York Data Dashboard (300.61 KB) More Documents & Publications Lowell, Massachusetts Data Dashboard Chicago Metropolitan Agency for Planning Data Dashboard Austin Energy Data Dashboard

  6. Geographically Based Hydrogen Consumer Demand and Infrastructure Analysis: Final Report

    SciTech Connect (OSTI)

    Melendez, M.; Milbrandt, A.

    2006-10-01

    In FY 2004 and 2005, NREL developed a proposed minimal infrastructure to support nationwide deployment of hydrogen vehicles by offering infrastructure scenarios that facilitated interstate travel. This report identifies key metropolitan areas and regions on which to focus infrastructure efforts during the early hydrogen transition.

  7. Papers Presented - Geothermal Resources Council 1980 Annual Meeting

    SciTech Connect (OSTI)

    1980-10-01

    This report contains preprints of papers pertaining to geothermal energy development in the Eastern United States written by members of the Center for Metropolitan Planning and Research (Metro Center) and by the Applied Physics Laboratory (APL) both of The Johns Hopkins University.

  8. Demand, Supply, and Price Outlook for Reformulated Motor Gasoline 1995

    Reports and Publications (EIA)

    1994-01-01

    Provisions of the Clean Air Act Amendments of 1990 designed to reduce ground-level ozone will increase the demand for reformulated motor gasoline in a number of U.S. metropolitan areas. This article discusses the effects of the new regulations on the motor gasoline market and the refining industry.

  9. Energy in the urban environment. Proceedings of the 22. annual Illinois energy conference

    SciTech Connect (OSTI)

    1994-12-31

    The conference addressed the energy and environmental challenges facing large metropolitan areas. The topics included a comparison of the environmental status of cities twenty years ago with the challenges facing today`s large cities, sustainable economic development, improving the energy and environmental infrastructure, and the changing urban transportation sector. Selected papers are indexed separately for inclusion in the Energy Science and Technology Database.

  10. EA-2000: Proposed Land Transfer to Develop a General Aviation Airport at the East Tennessee Technology Park Heritage Center, Oak Ridge, Tennessee

    Broader source: Energy.gov [DOE]

    DOE is preparing an EA to assess potential environmental impacts of the proposed land transfer to the Metropolitan Knoxville Airport Authority for the development of a general aviation airport at the East Tennessee Technology Park Heritage Center, in Oak Ridge, Tennessee.

  11. CX-000619: Categorical Exclusion Determination

    Office of Energy Efficiency and Renewable Energy (EERE)

    Oklahoma State Energy Program American Recovery and Reinvestment Act - Metropolitan Tulsa Transit AuthorityCX(s) Applied: B5.1Date: 01/20/2010Location(s): Tulsa, OklahomaOffice(s): Energy Efficiency and Renewable Energy, Golden Field Office

  12. Emergency Preparedness

    Broader source: Energy.gov [DOE]

    The collection of Emergency Procedures documents for the Department of Energy, Headquarters buildings, in the Washington, DC, metropolitan area. All of these documents are only available to computers attached to the DOE Network. They are for use only by DOE Headquarters employees. • Emergency Procedures Pamphlets • Building Evacuation Routes • Occupant Emergency Plans (OEP's)

  13. Iowa Water and Wastewater Operators Seek SEP Certification in New Pilot Program

    Broader source: Energy.gov [DOE]

    The Des Moines Metropolitan Wastewater Reclamation Authority (WRA) and Des Moines Water Works have committed to participate in the Superior Energy Performance (SEP) program as the first members of a new program pilot. SEP recognizes facilities that meet the ISO 50001 global energy management standard and demonstrate improved energy performance.

  14. Idling is Not the Way to Go

    SciTech Connect (OSTI)

    2013-06-01

    Researchers estimate that idling from heavy-duty and light-duty vehicles combined wastes about 6 billion gallons of fuel annually. Many states have put restrictions on idling, especially in metropolitan areas. Clearly, idling is not the way to go.

  15. Life Cycle Nitrogen Trifluoride Emissions from Photovoltaics

    SciTech Connect (OSTI)

    Fthenakis, V.

    2010-10-25

    Amorphous- and nanocrystalline-silicon thin-film photovoltaic modules are made in high-throughput manufacturing lines that necessitate quickly cleaning the reactor. Using NF{sub 3}, a potent greenhouse gas, as the cleaning agent triggered concerns as recent reports reveal that the atmospheric concentrations of this gas have increased significantly. We quantified the life-cycle emissions of NF{sub 3} in photovoltaic (PV) manufacturing, on the basis of actual measurements at the facilities of a major producer of NF{sub 3} and of a manufacturer of PV end-use equipment. From these, we defined the best practices and technologies that are the most likely to keep worldwide atmospheric concentrations of NF{sub 3} at very low radiative forcing levels. For the average U.S. insolation and electricity-grid conditions, the greenhouse gas (GHG) emissions from manufacturing and using NF{sub 3} in current PV a-Si and tandem a-Si/nc-Si facilities add 2 and 7 g CO{sub 2eq}/kWh, which can be displaced within the first 1-4 months of the PV system life.

  16. Anomalous magnetic behavior in nanocomposite materials of reduced graphene oxide-Ni/NiFe{sub 2}O{sub 4}

    SciTech Connect (OSTI)

    Kollu, Pratap E-mail: anirmalagrace@vit.ac.in; Prathapani, Sateesh; Varaprasadarao, Eswara K.; Mallick, Sudhanshu; Bahadur, D. E-mail: anirmalagrace@vit.ac.in; Santosh, Chella; Grace, Andrews Nirmala E-mail: anirmalagrace@vit.ac.in

    2014-08-04

    Magnetic Reduced Graphene Oxide-Nickel/NiFe{sub 2}O{sub 4} (RGO-Ni/NF) nanocomposite has been synthesized by one pot solvothermal method. Respective phase formations and their purities in the composite are confirmed by High Resolution Transmission Electron Microscope and X Ray Diffraction, respectively. For the RGO-Ni/NF composite material finite-size effects lead to the anomalous magnetic behavior, which is corroborated in temperature and field dependent magnetization curves. Here, we are reporting the behavior of higher magnetization values for Zero Field Cooled condition to that of Field Cooled for the RGO-Ni/NF nanocomposite. Also, the observed negative and positive moments in Hysteresis loops at relatively smaller applied fields (100?Oe and 200?Oe) are explained on the basis of surface spin disorder.

  17. Worksheet

    U.S. Energy Information Administration (EIA) Indexed Site

    4,"Ames City of",6,1,"Omaha Public Power District","NF",127,0,0,6037,6037 554,"Ames City of",6,2,"MidAmerican Energy Co.","NF",52,0,0,3171,3171 554,"Ames City of",6,3,"Iowa State University","NF",62,0,0,2661,2661 590,"Anaheim City of",6,1,"American Electric Power",,7592,,,343620,343620 590,"Anaheim City of",6,2,"Arizona Public Service C0.",,41600,,,583600,583600

  18. Propionic acid production by extractive fermentation. 1. Solvent considerations

    SciTech Connect (OSTI)

    Gu, Z.; Glatz, B.A.; Glatz, C.E.

    1998-02-20

    Solvent selection for extractive fermentation for propionic acid was conducted with three systems: Alamine{reg_sign} 304-1 (trilaurylamine) in 2-octanol, 1-dodecanol, and Witcohol{reg_sign} 85 NF (oleyl alcohol). Among them, the solvent containing 2-octanol exhibited the highest partition coefficient in acid extraction, but it was also toxic to propionibacteria. The most solvent-resistant strain among five strains of the microorganism was selected. Solvent toxicity was eliminated via two strategies: entrapment of dissolved toxic solvent in the culture growth medium with vegetable oils such as corn, olive, or soybean oils; or replacement of the toxic 2-octanol with nontoxic Witcohol 85 NF. The complete recovery of acids from the Alamine 304-1/Witcohol 85 NF was also realized with vacuum distillation.

  19. Consider nanofiltration for membrane separations

    SciTech Connect (OSTI)

    Raman, L.P. ); Cheryna, M.; Rajagopalan, N. )

    1994-03-01

    The best known liquid-phase membrane processes are reverse osmosis (RO), ultrafiltration (UF), microfiltration (MF), dialysis, and electrodialysis (ED). However, over the past few years, a new membrane process called nanofiltration (NF) has emerged that promises to significantly widen the application of membranes in liquid-phase separations. This paper discusses the following: NF operating range, membrane properties, and the following applications: demineralizing water, cleaning up contaminated groundwater, ultrapure water, effluents containing heavy metals, offshore oil platforms, yeast production, pulp and paper mills, textile production, electroless copper plating, and cheese whey production.

  20. Report on thermal aging effects on tensile properties of ferritic-martensitic steels.

    SciTech Connect (OSTI)

    Li, M.; Soppet, W.K.; Rink, D.L.; Listwan, J.T.; Natesan, K.

    2012-05-10

    This report provides an update on the evaluation of thermal-aging induced degradation of tensile properties of advanced ferritic-martensitic steels. The report is the first deliverable (level 3) in FY11 (M3A11AN04030103), under the Work Package A-11AN040301, 'Advanced Alloy Testing' performed by Argonne National Laboratory, as part of Advanced Structural Materials Program for the Advanced Reactor Concepts. This work package supports the advanced structural materials development by providing tensile data on aged alloys and a mechanistic model, validated by experiments, with a predictive capability on long-term performance. The scope of work is to evaluate the effect of thermal aging on the tensile properties of advanced alloys such as ferritic-martensitic steels, mod.9Cr-1Mo, NF616, and advanced austenitic stainless steel, HT-UPS. The aging experiments have been conducted over a temperature of 550-750 C for various time periods to simulate the microstructural changes in the alloys as a function of time at temperature. In addition, a mechanistic model based on thermodynamics and kinetics has been used to address the changes in microstructure of the alloys as a function of time and temperature, which is developed in the companion work package at ANL. The focus of this project is advanced alloy testing and understanding the effects of long-term thermal aging on the tensile properties. Advanced materials examined in this project include ferritic-martensitic steels mod.9Cr-1Mo and NF616, and austenitic steel, HT-UPS. The report summarizes the tensile testing results of thermally-aged mod.9Cr-1Mo, NF616 H1 and NF616 H2 ferritic-martensitic steels. NF616 H1 and NF616 H2 experienced different thermal-mechanical treatments before thermal aging experiments. NF616 H1 was normalized and tempered, and NF616 H2 was normalized and tempered and cold-rolled. By examining these two heats, we evaluated the effects of thermal-mechanical treatments on material microstructures and

  1. http://www.ornl.gov/Procurement.PDF

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    8/93 1. All Grade 5 and Grade 8 fasteners of foreign origin which do not bear any manufacturer's headmarks: Grade 5 Grade 8 2. Grade 5 fasteners with the following manufacturers' headmarks: J Jinn Her (TW) KS Kosaka Kogyo (JP) J KS 3. Grade 8 fasteners with the following manufacturers' headmarks: A Asahi Mfg (JP) KS Kosaka Kogyo (JP) A KS NF RT NF Nippon Fasteners (JP) RT Takai Ltd (JP) H Hinomoto Metal (JP) FM Fastener Co (of JP) H FM J M MS KY M Minamida Sieybo (JP) KY Kyoei (JP) MS Minato

  2. Nuclear factor-?B is a common upstream signal for growth differentiation factor-5 expression in brown adipocytes exposed to pro-inflammatory cytokines and palmitate

    SciTech Connect (OSTI)

    Hinoi, Eiichi; Iezaki, Takashi; Ozaki, Kakeru; Yoneda, Yukio

    2014-10-03

    Highlights: GDF5 expression is up-regulated by IL-1?, TNF-? and palmitate in brown pre-adipocytes. NF-?B stimulates promoter activity and expression of GDF5 in brown pre-adipocytes. Recruitment of NF-?B to the GDF5 promoter is facilitated in BAT from ob/ob mice. An NF-?B inhibitor prevents upregulation of GDF5 expression in brown pre-adipocytes. - Abstract: We have previously demonstrated that genetic and acquired obesity similarly led to drastic upregulation in brown adipose tissue (BAT), rather than white adipose tissue, of expression of both mRNA and corresponding protein for the bone morphogenic protein/growth differentiation factor (GDF) member GDF5 capable of promoting brown adipogenesis. In this study, we evaluated expression profiles of GDF5 in cultured murine brown pre-adipocytes exposed to pro-inflammatory cytokines and free fatty acids (FFAs), which are all shown to play a role in the pathogenesis of obesity. Both interleukin-1? (IL-1?) and tumor necrosis factor-? (TNF-?) were effective in up-regulating GDF5 expression in a concentration-dependent manner, while similar upregulation was seen in cells exposed to the saturated FFA palmitate, but not to the unsaturated FFA oleate. In silico analysis revealed existence of the putative nuclear factor-?B (NF-?B) binding site in the 5?-flanking region of mouse GDF5, whereas introduction of NF-?B subunits drastically facilitated both promoter activity and expression of GDF5 in brown pre-adipocytes. Chromatin immunoprecipitation analysis confirmed significant facilitation of the recruitment of NF-?B to the GDF5 promoter in lysed extracts of BAT from leptin-deficient ob/ob obese mice. Upregulation o GDF5 expression was invariably inhibited by an NF-?B inhibitor in cultured brown pre-adipocytes exposed to IL-1?, TNF-? and palmitate. These results suggest that obesity leads to upregulation of GDF5 expression responsible for the promotion of brown adipogenesis through a mechanism relevant to activation of the

  3. Use of nanofiltration to reduce cooling tower water consumption.

    SciTech Connect (OSTI)

    Altman, Susan Jeanne; Ciferno, Jared

    2010-10-01

    Nanofiltration (NF) can effectively treat cooling-tower water to reduce water consumption and maximize water usage efficiency of thermoelectric power plants. A pilot is being run to verify theoretical calculations. A side stream of water from a 900 gpm cooling tower is being treated by NF with the permeate returning to the cooling tower and the concentrate being discharged. The membrane efficiency is as high as over 50%. Salt rejection ranges from 77-97% with higher rejection for divalent ions. The pilot has demonstrated a reduction of makeup water of almost 20% and a reduction of discharge of over 50%.

  4. Use of nanofiltration to reduce cooling tower water usage.

    SciTech Connect (OSTI)

    Sanchez, Andres L.; Everett, Randy L.; Jensen, Richard Pearson; Cappelle, Malynda A.; Altman, Susan Jeanne

    2010-09-01

    Nanofiltration (NF) can effectively treat cooling-tower water to reduce water consumption and maximize water usage efficiency of thermoelectric power plants. A pilot is being run to verify theoretical calculations. A side stream of water from a 900 gpm cooling tower is being treated by NF with the permeate returning to the cooling tower and the concentrate being discharged. The membrane efficiency is as high as over 50%. Salt rejection ranges from 77-97% with higher rejection for divalent ions. The pilot has demonstrated a reduction of makeup water of almost 20% and a reduction of discharge of over 50%.

  5. Multi-Attribute Decision Theory methodology for pollution control measure analysis

    SciTech Connect (OSTI)

    Barrera Roldan, A.S.; Corona Juarez, A. ); Hardie, R.W.; Thayer, G.R. )

    1992-01-01

    A methodology based in Multi-Attribute Decision Theory was developed to prioritize air pollution control measures and strategies (a set of measures) for Mexico City Metropolitan Area (MCMA). We have developed a framework that takes into account economic, technical feasibility, environmental, social, political, and institutional factors to evaluate pollution mitigation measures and strategies utilizing a decision analysis process. In a series of meetings with a panel of experts in air pollution from different offices of the mexican government we have developed General and Specific criteria for a decision analysis tree. With these tools the measures or strategies can be graded and a figure of merit can be assigned to each of them, so they can be ranked. Two pollution mitigation measures were analyzed to test the methodology, the results are presented. This methodology was developed specifically for Mexico City, though the experience gained in this work can be used to develop similar methodologies for other metropolitan areas throughout the world.

  6. Multi-Attribute Decision Theory methodology for pollution control measure analysis

    SciTech Connect (OSTI)

    Barrera Roldan, A.S.; Corona Juarez, A.; Hardie, R.W.; Thayer, G.R.

    1992-12-31

    A methodology based in Multi-Attribute Decision Theory was developed to prioritize air pollution control measures and strategies (a set of measures) for Mexico City Metropolitan Area (MCMA). We have developed a framework that takes into account economic, technical feasibility, environmental, social, political, and institutional factors to evaluate pollution mitigation measures and strategies utilizing a decision analysis process. In a series of meetings with a panel of experts in air pollution from different offices of the mexican government we have developed General and Specific criteria for a decision analysis tree. With these tools the measures or strategies can be graded and a figure of merit can be assigned to each of them, so they can be ranked. Two pollution mitigation measures were analyzed to test the methodology, the results are presented. This methodology was developed specifically for Mexico City, though the experience gained in this work can be used to develop similar methodologies for other metropolitan areas throughout the world.

  7. Municipal solid waste to electricity recommendations for project in Bangkok, Thailand

    SciTech Connect (OSTI)

    Not Available

    1985-09-01

    Bangkok Metropolitan Administration (BMA) is charged with the responsibility of public cleansing and waste disposal in the metropolitan area. BMA operates 600 trucks which collect the waste twice a day and dump it at its three sites located in the Huai Kwong, Bangkok Noi and Rataburana districts. Presently these trucks collect 3,000 metric tons of garbage per day. At the waste dump sites, which are rapidly overflowing, BMA presently operates four compost plants, three with input capacity of 320 tons per day each and a fourth which uses about 160 tons of garbage per day--thus utilizing about 1,120 tons of garbage per day. Creation of new sites would require going even farther away from the city, resulting in excessive transportation costs.

  8. Global Assessment of Hydrogen Technologies – Tasks 3 & 4 Report Economic, Energy, and Environmental Analysis of Hydrogen Production and Delivery Options in Select Alabama Markets: Preliminary Case Studies

    SciTech Connect (OSTI)

    Fouad, Fouad H.; Peters, Robert W.; Sisiopiku, Virginia P.; Sullivan Andrew J.; Gillette, Jerry; Elgowainy, Amgad; Mintz, Marianne

    2007-12-01

    This report documents a set of case studies developed to estimate the cost of producing, storing, delivering, and dispensing hydrogen for light-duty vehicles for several scenarios involving metropolitan areas in Alabama. While the majority of the scenarios focused on centralized hydrogen production and pipeline delivery, alternative delivery modes were also examined. Although Alabama was used as the case study for this analysis, the results provide insights into the unique requirements for deploying hydrogen infrastructure in smaller urban and rural environments that lie outside the DOE’s high priority hydrogen deployment regions. Hydrogen production costs were estimated for three technologies – steam-methane reforming (SMR), coal gasification, and thermochemical water-splitting using advanced nuclear reactors. In all cases examined, SMR has the lowest production cost for the demands associated with metropolitan areas in Alabama. Although other production options may be less costly for larger hydrogen markets, these were not examined within the context of the case studies.

  9. CRIBB Communications | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    CRIBB Communications CRIBB Communications This presentation comes from the U.S. Department of Energy Better Buildings Neighborhood Program and the Chicago Metropolitan Agency for Planning. CRIBB Communications (1.83 MB) More Documents & Publications Motivating Home Energy Improvements-Focus Groups for the U.S. Department of Energy Workforce Development and Sales Training for EE Contractors Transcript.doc Five Steps to a Profitable Contractor Base

  10. Students from Pueblo Triumph in Colorado Science Bowl

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Pueblo Triumph in Colorado Science Bowl For more information contact: e:mail: Public Affairs Golden, Colo., Feb. 28, 1998 — Students from Pueblo South High School won top honors at the 1998 Colorado Science Bowl today at Metropolitan State College in Denver. In the final round of rapid fire questions about physics, math, biology, astronomy, chemistry, computers and the earth sciences, students from Pueblo South High School were victorious over Pueblo Centennial High School. Forty-two student