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Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

Norwegian house mice (Mus musculus musculus/domesticus): distributions, routes of  

E-Print Network [OSTI]

Norwegian house mice (Mus musculus musculus/domesticus): distributions, routes of colonization commensal subspecies of house mouse in Norway: Mus musculus domesticus and M. m. musculus. Five nuclear, reflecting passive human transport to Norway, probably during the Viking period. M. m. musculus may have

Nachman, Michael

2

Comparing disease expression across species: an examination of radiation and species specific disease expression in Mus musculus and Peromyscus leucopus  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

disease expression across species: an examination of radiation and species specific disease expression across species: an examination of radiation and species specific disease expression in Mus musculus and Peromyscus leucopus William Liu 1 , Benjamin Haley 1 , Mary J. Kwasny 2 , Tatjana Paunesku 1 , Gayle Woloschak 1 1. Department of Radiation Oncology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611 2. Department of Preventative Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611 From 1969 to 1992, the Janus program at Argonne National Laboratory performed a large series of radiobiology experiments, examining the effects of varying doses of neutron and gamma radiation on two disparate species of mice, Mus musculus and Peromyscus leucopus. Much of this data has since been digitized and made freely accessible online. This study aims to revisit

3

The chromatin structure of endogenous MTV proviruses in Mus musculus  

E-Print Network [OSTI]

was performed with the nick translated DNA probes pMTV1 and pTC8. When Southern blots of Eco Rl digested DNAs were probed with these two probes combined, all MTV sequences were detected. To map the sites of hypersensitive restriction enzyme cleavage... Constructions Plasmids pMTV1 (Ucker et al. , 1981) and pTC8 (Peterson et al. , 1985), which together are complementary to the entire MTV genome, were nick translated and used to detect all MTV sequences. Probes pMTV3. 3 and pMTV2. 2 (Peterson, 1985) were...

Marich, James Edward

2012-06-07T23:59:59.000Z

4

Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice  

E-Print Network [OSTI]

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic targeting for microcircuit dissection and disease modeling. The introduction ...

Brunetti, Philip M.

5

Chromosomes and DNA of Mus: Terminal DNA synthetic sequences in three species  

Science Journals Connector (OSTI)

The DNA replication patterns of the terminal S phase of three species of Mus were analyzed by tritiated thymidine autoradiography. The centromeric heterochromatin of M. fulvidiventris is the latest component to f...

T. C. Hsu; Amara Markvong

6

Genomic analysis of mouse tumorigenesis  

E-Print Network [OSTI]

The availability of the human and mouse genome sequences has spurred a growing interest in analyzing mouse models of human cancer using genomic techniques. Comparative genomic studies on mouse and human tumors can be ...

Tam, Mandy Chi-Mun

2006-01-01T23:59:59.000Z

7

The ecology of anthrax and coinfection trade-offs from an immunological perspective: seasonal aspects of host susceptibility  

E-Print Network [OSTI]

Phodopus  sungorus.  Functional  Ecology  23:979–988.  Mus  musculus.  Molecular  Ecology   Abu-­?Raddad,  L.  J.  transmission  of  parasites?  Ecology  Letters  12:528– de  

Cizauskas, Carrie Ann

2013-01-01T23:59:59.000Z

8

E-Print Network 3.0 - als3p protects mice Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

to mice and rats. In Utah, house mice (Mus musculus) and Norway rats (Rattus... of house mice and Norway rats. House mice are ... Source: Berryman Institute, Department of...

9

E-Print Network 3.0 - acyltransferase-2 protects mice Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Utah, house mice (Mus musculus) and Norway rats (Rattus... of house mice and Norway rats. House mice are ... Source: Berryman Institute, Department of Fisheries and Wildlife, Utah...

10

Sinkpad: A Malleable Mouse Pad Consisted of an Elastic Material  

E-Print Network [OSTI]

the mouse wheel, and moving the mouse. To solve this problem, it is necessary to augment conventional mouse. [9] described a two-ball mouse with an additional ball to capture angular movement to provide 3DOF

Tanaka, Jiro

11

E-Print Network 3.0 - acid receptor-1 gene Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Medicine 17 BioMed Central Page 1 of 15 Summary: (Ttr), Homo sapiens (FLJ13180 fis), Mus musculus estrogen receptor 1 (Esr1). 12;BMC Bioinformatics... Proceedings...

12

Mouse 2.0: Multi-touch Meets the Mouse Nicolas Villar1  

E-Print Network [OSTI]

by Doug Englebart et al. [9]. Since then, re- peated efforts have been made to augment the basic mouse

Benko, Hrvoje

13

Mouse Models of Gynecologic Pathology  

Science Journals Connector (OSTI)

Over the past two decades, the cataloguing of mutated oncogenes and tumor-suppressor genes in various types of human cancers has provided a rich knowledge base for the creation of genetic models. Although powerful in terms of their ability to elucidate gene function in a controlled environment in... Two new mouse models have been devised that shed light on the genesis of endometriosis and endometrioid ovarian cancer, suggest drugs for the treatment of endometrioid ovarian cancer, and provide a means of testing them.

Boyd J.

2005-05-26T23:59:59.000Z

14

Characterization of the Mouse Brain Proteome Using Global Proteomic...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the Mouse Brain Proteome Using Global Proteomic Analysis Complemented with Cysteinyl-Peptide Enrichment. Characterization of the Mouse Brain Proteome Using Global Proteomic...

15

Initial sequencing and comparative analysis of the mouse genome  

E-Print Network [OSTI]

Initial sequencing and comparative analysis of the mouse genome Mouse Genome Sequencing Consortium ........................................................................................................................................................................................................................... The sequence of the mouse genome is a key informational tool for understanding the contents of the human genome collaboration to produce a high-quality draft sequence of the mouse genome. We also present an initial

Eddy, Sean

16

Dazl regulates mouse embryonic germ cell development  

E-Print Network [OSTI]

In the mouse, germ cells can undergo differentiation to become either oocytes or spermatozoa in response to sex of their gonadal environment. The nature of the germ cell-intrinsic aspects of this signaling have not been ...

Gill, Mark E

2010-01-01T23:59:59.000Z

17

AN XXY MALE IN THE MOUSE  

Science Journals Connector (OSTI)

...RUSSELL AND ERNEST H. Y. CHU BIOLOGY DIVISION, OAK RIDGE NATIONAL LABORATORY,* OAK RIDGE, TENNESSEE Communicated by Alexander Hollaender...exceptional male is illustrated in Figure 1. Other workers have not definitely identified the Y in the mouse...

Liane Brauch Russell; Ernest H. Y. Chu

1961-01-01T23:59:59.000Z

18

Surfing the internet with a BCI mouse  

Science Journals Connector (OSTI)

In this paper, we present a new web browser based on a two-dimensional (2D) brain–computer interface (BCI) mouse, where our major concern is the selection of an intended target in a multi-target web page. A real-world web page may contain tens or even hundreds of targets, including hyperlinks, input elements, buttons, etc. In this case, a target filter designed in our system can be used to exclude most of those targets of no interest. Specifically, the user filters the targets of no interest out by inputting keywords with a P300-based speller, while keeps those containing the keywords. Such filtering largely facilitates the target selection task based on our BCI mouse. When there are only several targets in a web page (either an original sparse page or a target-filtered page), the user moves the mouse toward the target of interest using his/her electroencephalographic signal. The horizontal movement and vertical movement are controlled by motor imagery and P300 potential, respectively. If the mouse encounters a target of no interest, the user rejects it and continues to move the mouse. Otherwise the user selects the target and activates it. With the collaboration of the target filtering and a series of mouse movements and target selections/rejections, the user can select an intended target in a web page. Based on our browser system, common navigation functions, including history rolling forward and backward, hyperlink selection, page scrolling, text input, etc, are available. The system has been tested on seven subjects. Experimental results not only validated the efficacy of the proposed method, but also showed that free internet surfing with a BCI mouse is feasible.

Tianyou Yu; Yuanqing Li; Jinyi Long; Zhenghui Gu

2012-01-01T23:59:59.000Z

19

Construction of Anatomically Correct Models of Mouse Brain Networks 1  

E-Print Network [OSTI]

Construction of Anatomically Correct Models of Mouse Brain Networks 1 B. H. McCormick a, W. Koh a Y and Public Health, Texas A&M University, 4458 TAMU, College Station, TX 77843-4458 Abstract The Mouse Brain Web, a federated database, provides for the construction of anatomically correct models of mouse brain

Choe, Yoonsuck

20

Neutron issues in the JANUS mouse program  

SciTech Connect (OSTI)

Over the last 25 years, the JANUS program in the Biological and Medical Research Division at Argonne National Laboratory (ANL) has compiled a database on the response of both sexes of an F{sub 1} hybrid mouse, the B6CF{sub 1} (C57BL/6 x BALB/c), to external whole- body irradiation by {sup 60}Co {gamma}-rays and fission neutrons. Three basic patterns of exposure for both neutrons and {gamma}-rays have been investigated: single exposures, 24 equal once-weekly exposures, and 60 equal once-weekly exposures. All irradiations were terminated at predetermined total doses, with dose calculated in centigrays at the midline of the mouse. Three endpoints will be discussed in this paper: (1) life shortening, (2) a point estimate for cumulative mortality, and (3) the hazard function. Life shortening is used as an analysis endpoint because it summarizes, in a single index, the integrated effect of all injuries accumulated by an organism. Histopathological analyses of the mice used in the ANL studies have indicated that 85% of the deaths were caused by neoplasms. Connective tissue tumors were the dominant tumor in the B6CF{sub 1} mouse, with tumors of lymphoreticular origin accounting for approximately 80% of this class. The latter two endpoints will therefore be used to describe the life table experience of mice dying from the lymphoreticular class of tumors. Dose-response models will be applied to the three endpoints in order to describe the response function for neutron exposures, evaluate the effect of dose range and pattern of exposure on the response function for neutrons, and provide a set of neutron relative biological effectiveness (RBE) values of the ANL database. 25 refs.

Carnes, B.A.; Grahn, D.

1990-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

Spatial Mapping of Protein Abundances in the Mouse Brain by Voxelation...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mapping of Protein Abundances in the Mouse Brain by Voxelation Integrated with High-Throughput Liquid Chromatography Spatial Mapping of Protein Abundances in the Mouse Brain by...

22

E-Print Network 3.0 - adult mouse white Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mouse white Search Powered by Explorit Topic List Advanced Search Sample search results for: adult mouse white Page: << < 1 2 3 4 5 > >> 1 Boston University Computer Science...

23

E-Print Network 3.0 - adult transgenic mouse Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mouse model engineered to investigate human brain-derived neurotrophic... transgenic lines have been obtained through microinjection of the YAC into fertilized mouse oocytes....

24

HumanMouse Gene Identification by Comparative Evidence Integration and  

E-Print Network [OSTI]

The identification of genes in the human genome remains a challenge, as the actual predictions appear to disagree of genes in the human genome by using a reference, such as mouse genome. However, this comparative genome. In particular, it is not clear whether the mouse is at the correct evolutionary distance from

Pavlovic, Vladimir

25

A novel sex determination system in a close relative of the house mouse  

Science Journals Connector (OSTI)

...Catzeflis, C. Gilbert, B. D. Hayter, J. A. J. Nel and...University of Stellenbosch to J.B.D. and three collaborative grants: CNRS-NRF (nos. 13293 and 15439), PICS...deletion portant sur le bras court d'un X chez Mus (Leggada) triton...

2010-01-01T23:59:59.000Z

26

Trb2, a mouse homolog of tribbles, is dispensable for kidney and mouse development  

SciTech Connect (OSTI)

Glomeruli comprise an important filtering apparatus in the kidney and are derived from the metanephric mesenchyme. A nuclear protein, Sall1, is expressed in this mesenchyme, and we previously reported that Trb2, a mouse homolog of Drosophila tribbles, is expressed in the mesenchyme-derived tissues of the kidney by microarray analyses using Sall1-GFP knock-in mice. In the present report, we detected Trb2 expression in a variety of organs during gestation, including the kidneys, mesonephros, testes, heart, eyes, thymus, blood vessels, muscle, bones, tongue, spinal cord, and ganglions. In the developing kidney, Trb2 signals were detected in podocytes and the prospective mesangium of the glomeruli, as well as in ureteric bud tips. However, Trb2 mutant mice did not display any apparent phenotypes and no proteinuria was observed, indicating normal glomerular functions. These results suggest that Trb2 plays minimal roles during kidney and mouse development.

Takasato, Minoru [Division of Integrative Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811 (Japan); Department of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Kobayashi, Chiyoko [Division of Integrative Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811 (Japan); Okabayashi, Koji [Department of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Kiyonari, Hiroshi; Oshima, Naoko [Laboratory for Animal Resources and Genetic Engineering, Center for Developmental Biology (CDB), RIKEN Kobe, 2-2-3 Minatojima Minami, Chuo-ku, Kobe 650-0047 (Japan); Asashima, Makoto [Department of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 (Japan); Nishinakamura, Ryuichi [Division of Integrative Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811 (Japan)], E-mail: ryuichi@gpo.kumamoto-u.ac.jp

2008-09-05T23:59:59.000Z

27

Insights from Human/Mouse genome comparisons  

SciTech Connect (OSTI)

Large-scale public genomic sequencing efforts have provided a wealth of vertebrate sequence data poised to provide insights into mammalian biology. These include deep genomic sequence coverage of human, mouse, rat, zebrafish, and two pufferfish (Fugu rubripes and Tetraodon nigroviridis) (Aparicio et al. 2002; Lander et al. 2001; Venter et al. 2001; Waterston et al. 2002). In addition, a high-priority has been placed on determining the genomic sequence of chimpanzee, dog, cow, frog, and chicken (Boguski 2002). While only recently available, whole genome sequence data have provided the unique opportunity to globally compare complete genome contents. Furthermore, the shared evolutionary ancestry of vertebrate species has allowed the development of comparative genomic approaches to identify ancient conserved sequences with functionality. Accordingly, this review focuses on the initial comparison of available mammalian genomes and describes various insights derived from such analysis.

Pennacchio, Len A.

2003-03-30T23:59:59.000Z

28

Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins  

Science Journals Connector (OSTI)

Proteomic analysis of gliosomes from mouse brain: identification and investigation of glial membrane proteins ...

Karen E. Carney; Marco Milanese; Pim van Nierop; Ka Wan Li; Stéphane H.R. Oliet; August B. Smit; Giambattista Bonanno; Mark H.G. Verheijen

2014-10-13T23:59:59.000Z

29

Ethanol Promotes Intestinal Tumorigenesis in the MIN Mouse  

Science Journals Connector (OSTI)

...Washington, DC Abstract 2872: Effects of ethanol and folate on peripubertal mouse mammary...consumption. The proposed mechanisms whereby ethanol exerts its effects include decreased folate...proposed mechanisms, we hypothesized that ethanol would have increased deleterious effects...

Hemant K. Roy; James M. Gulizia; William J. Karolski; Anne Ratashak; Michael F. Sorrell; Dean Tuma

2002-11-01T23:59:59.000Z

30

End Sequencing and Finger Printing of Human & Mouse BAC Libraries  

SciTech Connect (OSTI)

This project provided for continued end sequencing of existing and new BAC libraries constructed to support human sequencing as well as to initiate BAC end sequencing from the mouse BAC libraries constructed to support mouse sequencing. The clones, the sequences, and the fingerprints are now an available resource for the community at large. Research and development of new metaodologies for BAC end sequencing have reduced costs and increase throughput.

Fraser, C.

2005-09-27T23:59:59.000Z

31

ORIGINAL ARTICLE doi:10.1111/j.1558-5646.2007.00257.x  

E-Print Network [OSTI]

ORIGINAL ARTICLE doi:10.1111/j.1558-5646.2007.00257.x ASYMMETRY AND POLYMORPHISM OF HYBRID MALE-derived strains of Mus musculus and M. domesticus to examine F1 hybrid male sterility, one of the primary smaller testes and fewer sperm in hybrid male progeny of most crosses. Second, in some crosses hybrid male

Dean, Matthew D.

32

Low Dose Radiation Research Program: Identification of Mouse Genetic  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mouse Genetic Susceptibility to Radiation Carcinogenesis Mouse Genetic Susceptibility to Radiation Carcinogenesis Allan Balmain University of California, San Francisco San Francisco, CA. (Jointly funded by NASA and DOE) Why this Project? To identify pathways that control genetic susceptibility to radiation-induced DNA damage and tumor development using novel developments in genomics together with mouse genetics. Project Goals To identify genetic loci that trigger rapid tumor development of mice after radiation. To characterize new genes at these loci that act as tumor suppressor genes or oncogenes. Experimental Approach New candidate-radiation susceptibility genes will be identified using a unique haplotyping approach. Using DNA from radiation-induced lymphoma, changes in the gene copy number can be detected using BAC microarrays. The

33

E-Print Network 3.0 - aging mouse brain Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mouse brain Search Powered by Explorit Topic List Advanced Search Sample search results for: aging mouse brain Page: << < 1 2 3 4 5 > >> 1 www.sciencemag.org SCIENCE VOL 313 29...

34

Tandem Mass Spectrometry identifies many mouse brain O-GlcNAcylated...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Tandem Mass Spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase Tandem Mass Spectrometry identifies many mouse brain...

35

E-Print Network 3.0 - atdc5 mouse progenitor Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

con- firmed for mouse brain glia... astrocytes and the other, oligodendro- cytes. Comparison of O-2A Progenitors from Rat Optic Nerve and Mouse... and co-workers reported that...

36

Model 923-B Mouse Gas Anesthesia Head Holder Adaptor Adjustment Features  

E-Print Network [OSTI]

Model 923-B Mouse Gas Anesthesia Head Holder Adaptor Adjustment Features Dorsal/Ventral dial.352.3139 Toll Free: 1.877.352.3275 ^^ci&ion Q)e&i^n^^^r ^esea/H^/i Model 923-B Mouse Gas Anesthesia Head Holder 923B-1/07 #12;MODEL 923-B MOUSE GAS ANESTHESIA HEAD HOLDER The KOPF Mouse Gas Anesthesia Head Holder

Kleinfeld, David

37

Systematic Enhancement of Polymerization of Recombinant Sickle Hemoglobin Mutants: Implications for Transgenic Mouse Model  

E-Print Network [OSTI]

for Transgenic Mouse Model for Sickle Cell Anemia By Xianfeng Li, Urooj A. Mirza, Brian T. Chait, and James M for sisting of HbS, Hb Antilles, and Hb D-Punjab. Normal mouse sickle cell anemia, we have expressed generation of the transgenic mouse model for sickle cell and polymerized in a linear manner at progressively

Chait, Brian T.

38

Private and Public Antigens of the Mouse H-2 System  

Science Journals Connector (OSTI)

... of the antisera against public antigens but only very few reacted with antisera against the private antigens4. It could still have been argued that because the sample of wild mice ... which are widely shared by the alleles of the laboratory mouse6. The second group-the private antigens-consists of antigens H-2.2, 4, 9, 17, 19, 23 ...

JAN KLEIN

1971-02-26T23:59:59.000Z

39

Spontaneous Metastasis from Primary C3H Mouse Mammary Tumors  

Science Journals Connector (OSTI)

...2 sublines of the C3H strain, the exogenous (milk transmit ted) mouse mammary tumor virus-infectedC3H/He and the aforemen...140-147, 1983. 20. Cam, Y., Caulet, T., Bellon, G., Poulin, G., Legros, M., and Pytlinska, M. Immunohistochemical...

Jan Vaage and James P. Harlos

1987-01-15T23:59:59.000Z

40

Cytochemical Studies on the Mouse Mammary Tumor Virus  

Science Journals Connector (OSTI)

...Elaboration in C3H Mouse Mammary Car cinoma as Observed by...and Bernhard, W. Water Soluble Embedding Media...Hydroxypropyl Methacrylate, a New Water-miscible Embedding Medium...Bernhard, W. The Use of Water-soluble Glycol Methacrylate...Smith FIG. 5. Low-power micrograph showing the...

Gilbert H. Smith

1967-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Transplantable Mouse Neoplasm Control by Neutron Capture Therapy  

Science Journals Connector (OSTI)

... Elemental boron enriched to 96 per cent of boron-10 was converted to borax, and then to the more soluble sodium pentaborate. For injections ... intravenous injection in the mouse. Drug, sodium pentaborate decahydrate; dose, 35 mg as boron-10/g body weight. Each point represents an average obtained from ten animals. The analyses ...

LEE E. FARR; T. KONIKOWSKI

1967-07-29T23:59:59.000Z

42

Fundamental Ca2 Signaling Mechanisms in Mouse Dendritic  

E-Print Network [OSTI]

Fundamental Ca2 Signaling Mechanisms in Mouse Dendritic Cells: CRAC Is the Major Ca2 Entry Pathway, Meyer B. Jackson, David E. Clapham,* and Gerard P. Ahern2 Although Ca2 -signaling processes are thought to underlie many dendritic cell (DC) functions, the Ca2 entry pathways are unknown. Therefore, we investigated

Clapham, David E.

43

A small Handbook of Quantum Mechanics A. Nony Mous  

E-Print Network [OSTI]

A small Handbook of Quantum Mechanics A. Nony Mous "Seek simplicity and distrust it.". . . Alfred Spaces And All That 1.1 Introduction This handbook is meant to be a short introduction to Quantum of the mathematical formalism in a conceptual way. Among the major "discoveries" of physics in the twentieth century

Hart, Gus

44

A robust automated system elucidates mouse home cage behavioral structure  

E-Print Network [OSTI]

A robust automated system elucidates mouse home cage behavioral structure Evan H. Gouldinga,1 , A of behavior exhibited by mice in their home cages reflect the function and interaction of numerous behavioral of organization across diverse behaviors has been difficult to achieve and automate. Here, we describe

Jiggins, Francis

45

Structure and Emergence of Specific Olfactory Glomeruli in the Mouse  

E-Print Network [OSTI]

in the olfactory bulb. Connecting 1000 populations of OSNs to the 1800 glomeruli of the mouse bulb poses fluorescent protein (GFP) or a fusion of tau with GFP. We visualized OSNs and their axons in whole mounts-expressing OSNs occupies a large surface area of the bulb and coalesces abruptly into a proto- glomerulus

46

Mouse inbred strain differences in ethanol drinking to intoxication  

E-Print Network [OSTI]

Mouse inbred strain differences in ethanol drinking to intoxication J. S. Rhodes*, , M. M. Ford , C described a simple procedure, Drinking in the Dark (DID), in which C57BL/6J mice self-administer ethanol to a blood ethanol concentration (BEC) above 1 mg/ml. The test consists of replacing the water with 20

Garland Jr., Theodore

47

Mechanistic basis of infertility of mouse intersubspecific hybrids  

E-Print Network [OSTI]

Mechanistic basis of infertility of mouse intersubspecific hybrids Tanmoy Bhattacharyyaa , Sona to the Dobzhansky­Muller model, hybrid sterility is a consequence of the independent evolution of related taxa result- ing in incompatible genomic interactions of their hybrids. The model implies

Dean, Matthew D.

48

Low Dose Radiation Research Program: Adaptive Response of Mouse Skin  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Adaptive Response of Mouse Skin Epithelial Cells to Low Dose Adaptive Response of Mouse Skin Epithelial Cells to Low Dose Ionizing Radiation: Induction of NF-κB, MnSOD, 14-3-3ζ and Cyclin B1 Authors: Jian Jian Li, Kazi M. Ahmed, Ming Fan, Shaozhong Dong, Douglas R. Spitz, and Cheng-Rong Yu Institutions: Division of Molecular Radiobiology, Purdue University School of Health Sciences, West Lafayette, Indiana; Free Radical and Radiation Biology Program, Department of Radiation Oncology, Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, Iowa; Molecular Immunology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland Gene expression profiles demonstrate that a group of key stress-responsive genes are associated with radiation exposure and may contribute to cellular

49

An Anisotropic Fluid-Solid Model of the Mouse Heart  

SciTech Connect (OSTI)

A critical challenge in biomechanical simulations is the spatial discretization of complex fluid-solid geometries created from imaging. This is especially important when dealing with Lagrangian interfaces, as there must be at a minimum both geometric and topological compatibility between fluid and solid phases, with exact matching of the interfacial nodes being highly desirable. We have developed a solution to this problem and applied the approach to the creation of a 3D fluidsolid mesh of the mouse heart. First, a 50 micron isotropic MRI dataset of a perfusion-fixed mouse heart was segmented into blood, tissue, and background using a customized multimaterial connected fuzzy thresholding algorithm. Then, a multimaterial marching cubes algorithm was applied to produce two compatible isosurfaces, one for the blood-tissue boundary and one for the tissue-background boundary. A multimaterial smoothing algorithm that rigorously conserves volume for each phase simultaneously smoothed the isosurfaces. Next we applied novel automated meshing algorithms to generate anisotropic hybrid meshes with the number of layers and the desired element anisotropy for each material as the only input parameters. As the meshes are scale-invariant within a material and include boundary layer prisms, fluid-structure interaction computations would have a relative error equilibrated over the entire mesh. The resulting model is highly detailed mesh representation of the mouse heart, including features such as chordae and coronary vasculature, that is also maximally efficient to produce the best simulation results for the computational resources available

Carson, James P.; Kuprat, Andrew P.; Jiao, Xiangmin; del Pin, Facundo; Einstein, Daniel R.

2010-01-01T23:59:59.000Z

50

E-Print Network 3.0 - a140v mouse model Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Version 6. Official copy "Mouse" in Berkshire Encyclopedia of Human-Computer Interaction, W.S. Bainbridge (ed). 2004 by Berkshire Publishing Group. http:www.berkshirepublishing.c...

51

A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes  

Science Journals Connector (OSTI)

A mouse embryonic stem cell bank of inducible human chromosome 21 gene expression is shown to be an important resource for investigating trisomy.

Rossella De Cegli; Antonio Romito; Simona Iacobacci; Lei Mao; Mario Lauria; Anthony O Fedele; Joachim Klose; Christelle Borel; Patrick Descombes; Stylianos E Antonarakis; Diego di Bernardo; Sandro Banfi; Andrea Ballabio; Gilda Cobellis

2010-06-22T23:59:59.000Z

52

E-Print Network 3.0 - adult mouse tissues Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Shanghai Institute of Biological Sciences Collection: Biology and Medicine 25 A gene atlas of the mouse and human protein-encoding transcriptomes Summary: information on the...

53

Structure of mouse muskelin discoidin domain and biochemical characterization of its self-association  

Science Journals Connector (OSTI)

The structure of the discoidin domain from mouse muskelin has been determined and analysis of the interdomain interaction suggests a mechanism for self-association.

Kim, K.-H.

2014-10-16T23:59:59.000Z

54

E-Print Network 3.0 - adult mouse pancreas Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mouse. Since there are human... expressed in the adult pancreas (data not ... Source: Brand, Paul H. - Department of Physiology and Pharmacology, University of Toledo...

55

E-Print Network 3.0 - allergy mouse model Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

CBMC proliferation... -cockroach, anti-mouse, and anti-dust mite IgE levels, wheeze, cough, eczema and asthma. Results: ... Source: Columbia University, Department of...

56

Diarrhea as a cause of mortality in a mouse model of infectious colitis  

E-Print Network [OSTI]

Background: Comparative characterization of genome-wide transcriptional changes during infection can help elucidate the mechanisms underlying host susceptibility. In this study, transcriptional profiling of the mouse colon ...

Borenshtein, Diana

57

Development of a Three-Dimensional Ball Rotation Sensing System using Optical Mouse Sensors  

E-Print Network [OSTI]

to the case of a ball wheel. The system measures surface speed by using two or more optical mouse sensorsDevelopment of a Three-Dimensional Ball Rotation Sensing System using Optical Mouse Sensors Masaaki Kumagai and Ralph L. Hollis Abstract-- Robots using ball(s) as spherical wheels have the advantage

58

Prion Infection of Mouse Brain Reveals Multiple New Upregulated Genes Involved in Neuroinflammation or Signal Transduction  

Science Journals Connector (OSTI)

...research-article Prion Infection of Mouse Brain Reveals Multiple New Upregulated Genes Involved...response and signal transduction in mouse brain at various times after scrapie infection. In brains of scrapie-infected mice at pre- and post-clinical...

James A. Carroll; James F. Striebel; Brent Race; Katie Phillips; Bruce Chesebro

2014-12-10T23:59:59.000Z

59

Optical properties of the mouse eye Ying Geng,1,2,*  

E-Print Network [OSTI]

Optical properties of the mouse eye Ying Geng,1,2,* Lee Anne Schery,1 Robin Sharma,1,2 Alfredo, University of Rochester, Rochester, NY, 14627, USA 2 The Institute of Optics, University of Rochester indicate that the optical quality of the mouse eye assessed by measurement of its aberrations is remarkably

60

Conserved role for the serotonin transporter gene in rat and mouse neurobehavioral endophenotypes  

E-Print Network [OSTI]

Review Conserved role for the serotonin transporter gene in rat and mouse neurobehavioral. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 7.3. Positive emotionality: food intake and responsivity to psychostimulants: Received 2 May 2009 Received in revised form 12 August 2009 Accepted 13 August 2009 Keywords: Mouse Rat

Kalueff, Allan V.

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Janus Experiments: Data from Mouse Irradiation Experiments 1972 - 1989  

DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

The Janus Experiments, carried out at Argonne National Laboratory from 1972 to 1989 and supported by grants from the US Department of Energy, investigated the effects of neutron and gamma radiation on mouse tissues primarily from B6CF1 mice. 49,000 mice were irradiated: Death records were recorded for 42,000 mice; gross pathologies were recorded for 39,000 mice; and paraffin embedded tissues were preserved for most mice. Mouse record details type and source of radiation [gamma, neutrons]; dose and dose rate [including life span irradiation]; type and presence/absence of radioprotector treatment; tissue/animal morphology and pathology. Protracted low dose rate treatments, short term higher dose rate treatments, variable dose rates with a same total dose, etc. in some cases in conjunction with radioprotectors, were administered. Normal tissues, tumors, metastases were preserved. Standard tissues saved were : lung, liver, spleen, kidney, heart, any with gross lesions (including mammary glands, Harderian gland with eye, adrenal gland, gut, ovaries or testes, brain and pituitary, bone). Data are searchable and specimens can be obtained by request.

62

Transplantation of Adult Mouse iPS Cell-Derived Photoreceptor Precursors Restores Retinal Structure and Function in Degenerative Mice  

E-Print Network [OSTI]

al. ( 2007) Treatment of sickle cell anemia mouse model withan animal model of sickle cell anemia [36], or engraftment

2011-01-01T23:59:59.000Z

63

A Systematic Analysis of a Deep Mouse Epididymal Sperm Proteome  

SciTech Connect (OSTI)

Spermatozoa are highly specialized cells that, when mature, are capable of navigating the female reproductive tract and fertilizing an oocyte. The sperm cell is thought to be largely quiescent in terms of transcriptional and translational activity. As a result, once it has left the male reproductive tract, the sperm cell is essentially operating with a static population of proteins. It is therefore theoretically possible to understand the protein networks contained in a sperm cell and to deduce its cellular function capabilities. To this end we have performed a proteomic analysis of mouse sperm isolated from the cauda epididymis and have confidently identified 2,850 proteins, which is the most comprehensive sperm proteome for any species reported to date. These proteins comprise many complete cellular pathways, including those for energy production via glycolysis, ?-oxidation and oxidative phosphorylation, protein folding and transport, and cell signaling systems. This proteome should prove a useful tool for assembly and testing of protein networks important for sperm function.

Chauvin, Theodore; Xie, Fang; Liu, Tao; Nicora, Carrie D.; Yang, Feng; Camp, David G.; Smith, Richard D.; Roberts, Kenneth P.

2012-12-21T23:59:59.000Z

64

E-Print Network 3.0 - aged mouse ovaries Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ovaries Search Powered by Explorit Topic List Advanced Search Sample search results for: aged mouse ovaries Page: << < 1 2 3 4 5 > >> 1 Journal of Reproduction and Fertility (1994)...

65

E-Print Network 3.0 - adult mouse offspring Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Our strategy was to employ the A. fumigatus mouse model that induces... allergen and diesel exhaust exposure and their effects on allergy in adult offspring mice Lin Lin1,...

66

The Design and Evaluation of Multi-Finger Mouse Emulation Techniques  

E-Print Network [OSTI]

, Jessica Lo, George Fitzmaurice Autodesk Research 210 King St. East, Toronto, Ontario, Canada, M5A 1J7 {firstname.lastname}@autodesk.com ABSTRACT We explore the use of multi-finger input to emulate full mouse

Toronto, University of

67

Metabolism of Benzo(a)pyrene by Variant Mouse Hepatoma Cells  

Science Journals Connector (OSTI)

...L. S.] and Biology Division, Oak Ridge National Laboratory [J. K. S.], P. 0. Box Y, Oak Ridge, Tennessee 37831 ABSTRACT Four mouse hepato'ma...density agrees with the results of these workers, but the observation of some production...

Eugene L. Schaefer and James K. Selkirk

1985-08-01T23:59:59.000Z

68

Abstract 3714: Trametinib (Mekinist TM) has limited brain distribution in the mouse model  

Science Journals Connector (OSTI)

...Trametinib (Mekinist TM) has limited brain distribution in the mouse model Shruthi...molecularly-targeted agents into the brain. The discovery of focal BRAF mutations...vemurafenib and dabrafenib) have limited brain distribution due to active efflux at the...

Shruthi Vaidhyanathan; Rajendar K. Mittapalli; Daniel J. Ma; Mark A. Schroeder; Jann N. Sarkaria; William F. Elmquist

2014-10-01T23:59:59.000Z

69

E-Print Network 3.0 - adult mouse brain Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

brain Search Powered by Explorit Topic List Advanced Search Sample search results for: adult mouse brain Page: << < 1 2 3 4 5 > >> 1 NMR IN BIOMEDICINE NMR Biomed. 2004;17:613619...

70

E-Print Network 3.0 - adult mouse heart Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

heart Search Powered by Explorit Topic List Advanced Search Sample search results for: adult mouse heart Page: << < 1 2 3 4 5 > >> 1 Seminars in Cell & Developmental Biology 18...

71

E-Print Network 3.0 - ameliorates mouse liver Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Diverse Roles of Invariant Natural Killer T Cells in Liver Injury and Fibrosis Induced by Carbon Tetrachloride Summary: are abundant in iNKT cells.13-17 For example, mouse liver...

72

in vivo Photothermal Optical Coherence Tomography of Targeted Mouse Brain Tumors using Gold Nanostars  

Science Journals Connector (OSTI)

We present structural and molecular-contrast imaging of mouse brain tumors using photo-thermal optical coherence tomography (PT-OCT) in vivo. Based on strong PT response of gold...

Heo, Jung; Jang, Eunji; Haam, Seungjoo; Oh, Seung Jae; Huh, Yong-Min; Suh, Jin-Suck; Chung, Euiheon; Joo, Chulmin

73

MUS420/EE367A Lecture 12 Wave Digital Filters  

E-Print Network [OSTI]

resistances, we obtain the following wave digital filter elements (the elementary one-ports): Inductor: v(t) i a port resistance (for reasons we'll discuss later). · In matrix notation, we readily see

Smith III, Julius Orion

74

Abstract A05: The Mouse Tumor Biology (MTB) Database: An electronic tool for identifying and evaluating mouse and PDX models of human cancer  

Science Journals Connector (OSTI)

...human tumors and provides freely available web access to these data. MTB integrates data...cancer research community and has developed a web-based system to facilitate submission...MPD), the Biology of the Mammary Gland Web Site, and the NCI Mouse Repository. MTB...

Dale A. Begley; Debra M. Krupke; Steven B. Neuhauser; Joel E. Richardson; John P. Sundberg; Carol J. Bult; Janan T. Eppig

2014-11-01T23:59:59.000Z

75

Mouse mammary tumor virus uses mouse but not human transferrin receptor 1 to reach a low pH compartment and infect cells  

SciTech Connect (OSTI)

Mouse mammary tumor virus (MMTV) is a pH-dependent virus that uses mouse transferrin receptor 1 (TfR1) for entry into cells. Previous studies demonstrated that MMTV could induce pH 5-dependent fusion-from-with of mouse cells. Here we show that the MMTV envelope-mediated cell-cell fusion requires both the entry receptor and low pH (pH 5). Although expression of the MMTV envelope and TfR1 was sufficient to mediate low pH-dependent syncytia formation, virus infection required trafficking to a low pH compartment; infection was independent of cathepsin-mediated proteolysis. Human TfR1 did not support virus infection, although envelope-mediated syncytia formation occurred with human cells after pH 5 treatment and this fusion depended on TfR1 expression. However, although the MMTV envelope bound human TfR1, virus was only internalized and trafficked to a low pH compartment in cells expressing mouse TfR1. Thus, while human TfR1 supported cell-cell fusion, because it was not internalized when bound to MMTV, it did not function as an entry receptor. Our data suggest that MMTV uses TfR1 for all steps of entry: cell attachment, induction of the conformational changes in Env required for membrane fusion and internalization to an appropriate acidic compartment.

Wang Enxiu; Obeng-Adjei, Nyamekye; Ying Qihua [Department of Microbiology and Abramson Family Cancer Center, University of Pennsylvania, 313BRBII/III, 421 Curie Blvd., Philadelphia, PA 19104 (United States); Meertens, Laurent; Dragic, Tanya [Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY (United States); Davey, Robert A. [Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX (United States); Ross, Susan R. [Department of Microbiology and Abramson Family Cancer Center, University of Pennsylvania, 313BRBII/III, 421 Curie Blvd., Philadelphia, PA 19104 (United States)], E-mail: rosss@mail.med.upenn.edu

2008-11-25T23:59:59.000Z

76

Activation of farnesoid X receptor induces RECK expression in mouse liver  

SciTech Connect (OSTI)

Highlights: •RECK is a novel transcriptional target gene of FXR in mouse liver. •The FXR response element is located within the intron 1 of RECK gene. •FXR agonist reverses the down-regulation of RECK in the liver in mouse NASH model. -- Abstract: Farnesoid X receptor (FXR) belongs to the ligand-activated nuclear receptor superfamily, and functions as a transcription factor regulating the transcription of numerous genes involved in bile acid homeostasis, lipoprotein and glucose metabolism. In the present study, we identified RECK, a membrane-anchored inhibitor of matrix metalloproteinases, as a novel target gene of FXR in mouse liver. We found that FXR agonist substantially augmented hepatic RECK mRNA and protein expression in vivo and in vitro. FXR regulated the transcription of RECK through directly binding to FXR response element located within intron 1 of the mouse RECK gene. Moreover, FXR agonist reversed the down-regulation of RECK in the livers from mice fed a methionine and choline deficient diet. In summary, our data suggest that RECK is a novel transcriptional target of FXR in mouse liver, and provide clues to better understanding the function of FXR in liver.

Peng, Xiaomin [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China)] [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Wu, Weibin [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China) [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Institutes of Biomedical Sciences, Fudan University, Shanghai 200032 (China); Zhu, Bo; Sun, Zhichao; Ji, Lingling; Ruan, Yuanyuan [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China)] [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Zhou, Meiling, E-mail: meilingzhou2012@gmail.com [Department of Radiology, Zhongshan Hospital of Fudan University and Shanghai Institute of Medical Imaging, Shanghai 200032 (China)] [Department of Radiology, Zhongshan Hospital of Fudan University and Shanghai Institute of Medical Imaging, Shanghai 200032 (China); Zhou, Lei, E-mail: yhchloech@gmail.com [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China)] [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Gu, Jianxin [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China) [Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032 (China); Institutes of Biomedical Sciences, Fudan University, Shanghai 200032 (China)

2014-01-03T23:59:59.000Z

77

Mouse Strain-Dependent Variations in Sensitivity to Induction of Gamma-H2AX  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mouse Strain-Dependent Variations in Sensitivity to Induction of Gamma-H2AX Mouse Strain-Dependent Variations in Sensitivity to Induction of Gamma-H2AX Foci after Continuous Low Dose-Rate Irradiation: The Atm-/- vs Atm +/+ genotypes on Balb/c, 129S6, C57BL/6J, and A/J inbred strains J.R. Brogan Colorado State University Abstract We have recently developed a ‘low dose rate’ gamma-H2AX assay that is sufficiently sensitive to distinguish differences in response for cells from normal Atm +/+ (mouse) or ATM+/+ (human) and the phenotypes associated with the corresponding heterozygous genotypes(1, 2). The assay is also capable of distinguishing mild hypersensitivities for cells from an appreciable proportion of apparently normal individuals(3). We used this assay to determine whether the genetic background of four commonly used

78

Ultrastructural study of Rift Valley fever virus in the mouse model  

SciTech Connect (OSTI)

Detailed ultrastructural studies of Rift Valley fever virus (RVFV) in the mouse model are needed to develop and characterize a small animal model of RVF for the evaluation of potential vaccines and therapeutics. In this study, the ultrastructural features of RVFV infection in the mouse model were analyzed. The main changes in the liver included the presence of viral particles in hepatocytes and hepatic stem cells accompanied by hepatocyte apoptosis. However, viral particles were observed rarely in the liver; in contrast, particles were extremely abundant in the CNS. Despite extensive lymphocytolysis, direct evidence of viral replication was not observed in the lymphoid tissue. These results correlate with the acute-onset hepatitis and delayed-onset encephalitis that are dominant features of severe human RVF, but suggest that host immune-mediated mechanisms contribute significantly to pathology. The results of this study expand our knowledge of RVFV-host interactions and further characterize the mouse model of RVF.

Reed, Christopher; Steele, Keith E.; Honko, Anna; Shamblin, Joshua; Hensley, Lisa E. [United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States)] [United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States); Smith, Darci R., E-mail: darci.smith1@us.army.mil [United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD (United States)

2012-09-15T23:59:59.000Z

79

Laser-Assisted In Vitro Fertilization Facilitates Fertilization of Vitrified-Warmed C57BL/6 Mouse Oocytes with Fresh and Frozen-Thawed Spermatozoa, Producing Live Pups  

E-Print Network [OSTI]

The utility of cryopreserved mouse gametes for reproduction of transgenic mice depends on development of assisted reproductive technologies, including vitrification of unfertilized mouse oocytes. Due to hardening of the ...

Woods, Stephanie E.

80

Coxiella burnetii Isolates Cause Genogroup-Specific Virulence in Mouse and Guinea Pig Models of Acute Q Fever  

Science Journals Connector (OSTI)

...disease in the SCID mouse model, and genogroup-consistent...immunocompetent-mouse model. Guinea pigs developed...signs of (i) an acute flu-like illness with or...whether these groupings predict virulence potential and...but avirulent in rodent models of disease (47, 48...

K. E. Russell-Lodrigue; M. Andoh; M. W. J. Poels; H. R. Shive; B. R. Weeks; G. Q. Zhang; C. Tersteeg; T. Masegi; A. Hotta; T. Yamaguchi; H. Fukushi; K. Hirai; D. N. McMurray; J. E. Samuel

2009-09-28T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Screening for pain phenotypes: Analysis of three congenic mouse strains on a battery of nine nociceptive assays  

E-Print Network [OSTI]

Screening for pain phenotypes: Analysis of three congenic mouse strains on a battery of nine portion of the genome of the MOLF/Ei strain has been placed on a C57BL/6 genetic background ­ on a battery variability-causing genes), one for thermal nociception on mouse chromosome (Chr) 17 (Chr 17; Tpnr3) and one

Levitin, Daniel

82

Gene Expression Analysis Exposes Mitochondrial Abnormalities in a Mouse Model of Rett Syndrome  

Science Journals Connector (OSTI)

...and 0.4 M NaOH), the solution was overlaid with mineral oil and incubated for 5 h at 55C. Bisulfite-treated DNA was precipitated...Uqcrc1 coding sequence was PCR amplified from cDNA (Clontech MARATHON Ready mouse 11-day embryo cDNA) and cloned into pBABE Puro...

Skirmantas Kriaucionis; Andrew Paterson; John Curtis; Jacky Guy; Nikki MacLeod; Adrian Bird

2006-07-01T23:59:59.000Z

83

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain  

E-Print Network [OSTI]

Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain Hans entire neuronal networks for analysis in the intact brain has been impossible up to now. Techniques like in micro computer tomogrpahy and mMRI does not resolve individual cells1,2. On a microscopic scale

Cai, Long

84

Triclosan as a Systemic Antibacterial Agent in a Mouse Model of Acute Bacterial Challenge  

Science Journals Connector (OSTI)

...Microbiology ARTICLE EXPERIMENTAL THERAPEUTICS Triclosan as a Systemic Antibacterial Agent in...and/or use of effective antibiotics. Triclosan, though used in cosmetic and dermatological...Here we report the striking efficacy of triclosan in a mouse model of acute systemic bacterial...

Shilpi Sharma; T. N. C. Ramya; Avadhesha Surolia; Namita Surolia

2003-12-01T23:59:59.000Z

85

Combined MEK and PI3K inhibition in a mouse model of pancreatic cancer  

Science Journals Connector (OSTI)

...adenocarcinoma (PDAC). As dual MEK and PI3K inhibition is presently being employed in clinical trials for PDAC patients, we sought to test the efficacy of combined targeting of these pathways in PDAC using both in vitro drug screens and genetically engineered mouse...

Brinda Alagesan; Gianmarco Contino; Alexander R Guimaraes; Ryan B Corcoran; Vikram Desphande; Gregory R Wojtkiewicz; Aram F. Hezel; Kwok-Kin Wong; Massimo Loda; Ralph Weissleder; Cyril H Benes; Jeffrey A Engelman; Nabeel Bardeesy

86

Mouse regulatory DNA landscapes reveal global principles of cis-regulatory evolution  

Science Journals Connector (OSTI)

...within tissues. For example, the 91,951 shared brain-selective DHSs in turn comprised four subclusters corresponding...versus human regulatory T cell DHSs and for mouse brain versus human fetal brain. In each case a linear relationship is observed...

Jeff Vierstra; Eric Rynes; Richard Sandstrom; Miaohua Zhang; Theresa Canfield; R. Scott Hansen; Sandra Stehling-Sun; Peter J. Sabo; Rachel Byron; Richard Humbert; Robert E. Thurman; Audra K. Johnson; Shinny Vong; Kristen Lee; Daniel Bates; Fidencio Neri; Morgan Diegel; Erika Giste; Eric Haugen; Douglas Dunn; Matthew S. Wilken; Steven Josefowicz; Robert Samstein; Kai-Hsin Chang; Evan E. Eichler; Marella De Bruijn; Thomas A. Reh; Arthur Skoultchi; Alexander Rudensky; Stuart H. Orkin; Thalia Papayannopoulou; Piper M. Treuting; Licia Selleri; Rajinder Kaul; Mark Groudine; M. A. Bender; John A. Stamatoyannopoulos

2014-11-21T23:59:59.000Z

87

Interaction of mouse mammary epithelial cells with collagen substrata: regulation of casein gene expression and secretion  

Science Journals Connector (OSTI)

...polyvinyl pyrrolidone and then hybridized with 32P-labeled, nick-translated mouse caseins or whey acidic protein (WAP) cDNA...matrix of mammary cells (29) to that of casein gene expression merit further investigation. We thank Dr. Jeffrey M. Rosen for...

E Y Lee; W H Lee; C S Kaetzel; G Parry; M J Bissell

1985-01-01T23:59:59.000Z

88

Effect of Sex Hormones on Susceptibility to Viral Transmission of Myeloid Leukemia in the Mouse  

Science Journals Connector (OSTI)

...passages. In every passage, the material from a given donor was injected...myeloid leukemia intheRF mouse. Materials and Methods HANDLING OF MICE. The animals used were...for 20mm. CHART 1. Schematic diagram of procedure for preparing the...

V. K. Jenkins; T. T. Odell, Jr.; and A. C. Upton

1966-03-01T23:59:59.000Z

89

Heart valve cardiomyocytes of mouse embryos express the serotonin transporter SERT  

SciTech Connect (OSTI)

Multiple evidence demonstrate a role for serotonin and its transporter SERT in heart valve development and disease. By utilizing a Cre/loxP system driven by SERT gene expression, we recently demonstrated a regionally restricted distribution of SERT-expressing cells in developing mouse heart. In order to characterize the cell types exhibiting SERT expression within the mouse heart valves at early developmental stages, in this study we performed immunohistochemistry for Islet1 (Isl1) and connexin-43 (Cx-43) on heart sections from SERT{sup Cre/+};ROSA26R embryos previously stained with X-gal. We observed the co-localization of LacZ staining with Isl1 labelling in the outflow tract, the right ventricle and the conal region of E11.5 mouse heart. Cx-43 labelled cells co-localized with LacZ stained cells in the forming atrioventricular valves. These results demonstrate the cardiomyocyte phenotype of SERT-expressing cells in heart valves of the developing mouse heart, thus suggesting an active role of SERT in early heart valve development.

Pavone, Luigi Michele [Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino 1, 80137 Naples (Italy); Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Naples (Italy)], E-mail: pavone@dbbm.unina.it; Spina, Anna; Lo Muto, Roberta; Santoro, Dionea [Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino 1, 80137 Naples (Italy); Department of Biochemistry and Medical Biotechnologies, University of Naples Federico II, Naples (Italy); Mastellone, Vincenzo [Department of Experimental Medicine 'G. Salvatore', University of Magna Graecia, Catanzaro (Italy); Avallone, Luigi [Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via F. Delpino 1, 80137 Naples (Italy)

2008-12-12T23:59:59.000Z

90

SkyMouse: A smart interface for astronomical on-line resources and services  

E-Print Network [OSTI]

With the development of network and the World Wide Web (WWW), the Internet has been growing and changing dramatically. More and more on-line database systems and different kinds of services are available for astronomy research. How to help users find their way through the jungle of information services becomes an important challenge. Although astronomers have been aware of the importance of interoperability and introduced the concept of Virtual Observatory as a uniform environment for future astronomical on-line resources and services, transparent access to heterogeneous on-line information is still difficult. SkyMouse is a lightweight interface for distributed astronomical on-line resources and services, which is designed and developed by us, i.e., Chinese Virtual Observatory Project. Taking advantage of screen word-capturing technology, different kinds of information systems can be queried through simple mouse actions, and results are returned in a uniform web page. SkyMouse is an easy to use application, aiming to show basic information or to create a comprehensive overview of a specific astronomical object. In this paper current status of on-line resources and services access is reviewed; system architecture, features and functions of SkyMouse are described; challenges for intelligent interface for on-line astronomical resources and services are discussed.

Chen-Zhou CUI; Hua-Ping SUN; Yong-Heng ZHAO; Yu LUO; Da-Zhi QI

2007-11-27T23:59:59.000Z

91

Lack of T-Cell Immunity in Humans with Preexisting Anti-Mouse Immunoglobulin Reactivity  

Science Journals Connector (OSTI)

...nocarcinomas called TAG-72 (17), was a generous gift from Dr. Jeffery Schlom, NIH. Normal polyclonal mouse IgG and normal polyclonal...coiorectal cancer. J. Nucl. Med., 33: 23-29, 1992. 3. Benjamin, D. C., Berzofsky, J. A., East, I. J., Grud, F...

Robert M. Conry; M. B. Khazaeli; and Albert F. LoBuglio

1992-12-15T23:59:59.000Z

92

Metabolism and pharmacokinetics of the cyclin-dependent kinase inhibitor R-roscovitine in the mouse  

Science Journals Connector (OSTI)

...hepatic microsomes, plasma, and urine. Following...under an argon atmosphere cooled to 0C was...d6-DMSO, 250 MHz): delta 4.62...d6-DMSO, 250 MHz): delta 0.82...25-hour mouse plasma samples from treated...aliquots (33 muL) of plasma from three animals...

Bernard P. Nutley; Florence I. Raynaud; Stuart C. Wilson; Peter M. Fischer; Angela Hayes; Phyllis M. Goddard; Steven J. McClue; Michael Jarman; David P. Lane; and Paul Workman

2005-01-01T23:59:59.000Z

93

Behavior of Three Types of Ribovirus-Like Particles in Segregating Hamster × Mouse Somatic Hybrids  

Science Journals Connector (OSTI)

...fortuitous, fluctuations of R particle con- tent may well be correlated...Sci. USA 71 (1974) Virus Particles in Hamster X Mouse Somatic...e Of. z 'to Eli . , r en god ^ t ; I;; i ' ' ;t': U...100 nm. Fig. 2: R-type particles in a hamster melanoma cell...

Y. Yotsuyanagi; Boris Ephrussi

1974-01-01T23:59:59.000Z

94

Automated whole-genome multiple alignment of rat, mouse, and human  

SciTech Connect (OSTI)

We have built a whole genome multiple alignment of the three currently available mammalian genomes using a fully automated pipeline which combines the local/global approach of the Berkeley Genome Pipeline and the LAGAN program. The strategy is based on progressive alignment, and consists of two main steps: (1) alignment of the mouse and rat genomes; and (2) alignment of human to either the mouse-rat alignments from step 1, or the remaining unaligned mouse and rat sequences. The resulting alignments demonstrate high sensitivity, with 87% of all human gene-coding areas aligned in both mouse and rat. The specificity is also high: <7% of the rat contigs are aligned to multiple places in human and 97% of all alignments with human sequence > 100kb agree with a three-way synteny map built independently using predicted exons in the three genomes. At the nucleotide level <1% of the rat nucleotides are mapped to multiple places in the human sequence in the alignment; and 96.5% of human nucleotides within all alignments agree with the synteny map. The alignments are publicly available online, with visualization through the novel Multi-VISTA browser that we also present.

Brudno, Michael; Poliakov, Alexander; Salamov, Asaf; Cooper, Gregory M.; Sidow, Arend; Rubin, Edward M.; Solovyev, Victor; Batzoglou, Serafim; Dubchak, Inna

2004-07-04T23:59:59.000Z

95

Comparative Analysis of the -Like Globin Clusters in Mouse, Rat, and Human Chromosomes Indicates a  

E-Print Network [OSTI]

to GenBank/EMBL under accession no. AY016022.] Genome sequencing projects and comparative genome analysesComparative Analysis of the -Like Globin Clusters in Mouse, Rat, and Human Chromosomes Indicates -globin pseudogene. Comparative sequence analysis with the functional -globin loci at human Chromosome 16p

Miller, Webb

96

In vivo fluorescent imaging of the mouse retina using adaptive optics  

E-Print Network [OSTI]

in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack­Hartmann wave- front is a powerful tool that allows fol- lowing a single animal over time. A major problem with implementing (Dual Calypso laser, Co- bolt AB, Stockholm, Sweden). An entrance pupil is formed at the source

97

In Vivo Three-photon Calcium Imaging of Brain Activity from Layer 6 Neurons in Mouse Brain  

Science Journals Connector (OSTI)

We demonstrate three-photon microscopy (3PM) at 1300-nm excitation for imaging neuronal activity as deep as layer-6 (L6) of adult mouse cortex using genetically-encoded calcium...

Ouzounov, Dimitre G; Horton, Nicholas; Wang, Tianyu; Feng, Danielle; Nishimura, Nozomi; Xu, Chris

98

The mapping of natural boron in histological sections of mouse tissues by the use of neutron-capture radiography  

Science Journals Connector (OSTI)

Neutron-capture radiography has been applied to the mapping of natural boron in mouse histological sections. The method is based on the fact that the stable isotope boron-10 has an extremely large cross-section f...

Maria Laurent-Pettersson; Bertrand Delpech; Michel Thellier

1992-12-01T23:59:59.000Z

99

Rapid Structural Remodeling of Thalamocortical Synapses Parallels Experience-Dependent Functional Plasticity in Mouse Primary Visual Cortex  

E-Print Network [OSTI]

Monocular lid closure (MC) causes a profound shift in the ocular dominance (OD) of neurons in primary visual cortex (V1). Anatomical studies in both cat and mouse V1 suggest that large-scale structural rearrangements of ...

Coleman, Jason E.

100

The Consensus Coding Sequence (Ccds) Project: Identifying a Common Protein-Coding Gene Set for the Human and Mouse Genomes  

E-Print Network [OSTI]

Effective use of the human and mouse genomes requires reliable identification of genes and their products. Although multiple public resources provide annotation, different methods are used that can result in similar but ...

Kellis, Manolis

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

Low LET Radiation Alters the Fetal Epigenome of the Avy Mouse  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

LET Radiation Alters the Fetal Epigenome of the Avy Mouse LET Radiation Alters the Fetal Epigenome of the Avy Mouse Randy Jirtle Duke University Medical Center Abstract Exposure to high levels of ionizing radiation significantly alters the epigenome in somatic cells and gametes (Filkowski and others, 2009), yet it has not been determined if epigenomic modifications occur below 10 cGy. Epigenetic dysregulation during early development is mechanistically linked to the pathogenesis of adult-onset diseases (Jirtle and Skinner, 2007). Thus, we sought to determine whether low doses of ionizing radiation during early gestation affect susceptibility to adult-onset diseases by deregulating the establishment of DNA methylation patterns. Agouti viable yellow (Avy) mice were used to test this novel postulate because they are

102

The low dose damage response pathways in the mouse mammary glands depends  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

low dose damage response pathways in the mouse mammary glands depends low dose damage response pathways in the mouse mammary glands depends on genotype, tissue compartment, exposure regimen, and sampling times Joe Gray & Andrew Wyrobek Lawrence Berkeley National Laboratory Abstract The objectives of this research are to characterize the early and persistent low-dose and adaptive response (AR) damage surveillance networks in mammary glands of radiation sensitive and resistant strains of mice to identify the molecular signatures/mechanisms associated with nonlinear modifications of risk for mammary gland cancer. Our approach uses low-dose exposure regimens that have been reported to induce mammary gland cancer in sensitive strains to determine whether low-dose induced pathways are differentially expressed in epithelial or stromal cells and to determine

103

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary Genetic susceptibility to low-dose ionizing radiation in the mouse mammary gland as a means of understanding human risk for breast cancer Antoine M. Snijders Lawrence Berkeley National Laboratory Abstract Goal: Our goal is to develop an in vivo mechanistic model of genetic variation in the low-dose damage responses of mammary glands using inbred mice known to vary in their sensitivity to low-dose induced mammary gland cancer, and to develop molecular predictors for susceptibility or resistance to low-dose induced breast cancer. Background and Significance: It is increasingly believed that individuals differ in their genetic susceptibilities to environmental insults for diseases such as cancer. This concern is especially important for the large numbers of individuals receiving low-dose exposures in the nuclear energy

104

Gene Expression Analysis of Apoptosis and Oxidative Stress in Mouse Brain  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Analysis of Apoptosis and Oxidative Stress in Mouse Brain Analysis of Apoptosis and Oxidative Stress in Mouse Brain After Low-dose and Acute Radiation Exposure Daila Gridley Loma Linda University & Medical Center Abstract Purpose: 1) To examine the induction of oxidative stress and apoptosis-associated gene expression profiles in brain after whole-body irradiation with low-dose/low-dose-rate (LDR) photons and acute exposure to photons 2) to compare these radiation-induced effects with those produced by LDR and acute exposure to protons. Material and Methods: C57BL/6 mice were exposed to 2 Gy of photons or protons at 0.8 Gy/min and 0.9 Gy/min, respectively, both with and without pre-exposure to 0.01 Gy LDR γ-rays (57Co) at 0.03 cGy/h. Brain tissues were harvested and quick-frozen for analyses by quantitative RTPCR at 56

105

Human-mouse comparative genomics: successes and failures to reveal functional regions of the human genome  

SciTech Connect (OSTI)

Deciphering the genetic code embedded within the human genome remains a significant challenge despite the human genome consortium's recent success at defining its linear sequence (Lander et al. 2001; Venter et al. 2001). While useful strategies exist to identify a large percentage of protein encoding regions, efforts to accurately define functional sequences in the remaining {approx}97 percent of the genome lag. Our primary interest has been to utilize the evolutionary relationship and the universal nature of genomic sequence information in vertebrates to reveal functional elements in the human genome. This has been achieved through the combined use of vertebrate comparative genomics to pinpoint highly conserved sequences as candidates for biological activity and transgenic mouse studies to address the functionality of defined human DNA fragments. Accordingly, we describe strategies and insights into functional sequences in the human genome through the use of comparative genomics coupled wit h functional studies in the mouse.

Pennacchio, Len A.; Baroukh, Nadine; Rubin, Edward M.

2003-05-15T23:59:59.000Z

106

Comparative metabolism of phenobarbitone in the rat (CFE) and mouse (CF1)  

Science Journals Connector (OSTI)

The fate of orally administered [14C]phenobarbitone (40 mg/kg) was studied comparatively in CFE rats and CF1 mice. Metabolism and elimination were rapid. Hydroxylation was more complete in rats than in mice but the major metabolite in both species was p-hydroxyphenobarbitone, which was excreted mostly in the urine in both free and conjugated form. Unmetabolized phenobarbitone was excreted in the urine of both rat and mouse. Two minor metabolites appeared to be common to both species. The effect of pretreatment with non-radioactive phenobarbitone on the metabolism of [14C]phenobarbitone was minimal in the rat but afforded a twofold increase in p-hydroxylation in the mouse (in vivo). Despite the good yield of p-hydroxyphenobarbitone in the intact rat, [14C]phenobarbitone was not metabolized by liver-microsomal preparations from either untreated or phenobarbitone-induced rats.

J.V. Crayford; D.H. Hutson

1980-01-01T23:59:59.000Z

107

FUNCTIONAL AND MECHANISTIC STUDY OF DOT1L IN MOUSE EMBRYONIC HEMATOPOIESIS  

E-Print Network [OSTI]

recognized that two distinct populations of red cells circulate in the blood stream of mouse embryos. The first population, the “primitive” red cells, are extremely large, nucleated red cells, which originate from the yolk sac2. Shortly after that, a second... to our increasing knowledge over time. For example, at first the primitive red cells are thought to be always nucleated and have the same characteristics with the nucleated nonmammalian red cells. Also originally, people believed that definitive...

Feng, Yi

2012-12-31T23:59:59.000Z

108

Genomic Imprinting Variations in the Mouse Type 3 Deiodinase Gene Between Tissues and Brain Regions  

E-Print Network [OSTI]

, tissues were dissected, immediately frozen on dry ice and stored at –70 C until further use. Brain regions were identified and harvested according to the mouse atlas by Paxinos (30). Animal procedures were approved by the Institutional Animal Care and Use... paternal, allele. This suggests that expression and associated alterations in chromatin facil- itate homologous recombination. The result is also con- sistent with observations that suggest the existence of epi- genetic boundaries that are associated with a...

Martinez, M. Elena; Charalambous, Marika; Saferali, Aabida; Fiering, Steven; Naumova, Anna K.; St. Germain, Donald; Ferguson-Smith, Anne C.; Hernandez, Arturo

2014-09-18T23:59:59.000Z

109

Saving Money and Fuel with a Click of a Mouse | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

Money and Fuel with a Click of a Mouse Money and Fuel with a Click of a Mouse Saving Money and Fuel with a Click of a Mouse January 10, 2012 - 4:19pm Addthis A look at tools that can help consumers save money and fuel, whether you’re in the market for a new vehicle or trying to make the most of your current one. | Photo courtesy of Auto Guide. A look at tools that can help consumers save money and fuel, whether you're in the market for a new vehicle or trying to make the most of your current one. | Photo courtesy of Auto Guide. Patrick B. Davis Patrick B. Davis Vehicle Technologies Program Manager What does this mean for me? Quickly compare cars based on their projected fuel costs, price, safety ratings and MPG. Calculate the long-term costs of your next vehicle. Discover simple tips and tricks that can start saving you fuel and

110

Dynamic changes in the distribution and time course of blood–brain barrier-permeative nitroxides in the mouse head with EPR imaging: visualization of blood flow in a mouse model of ischemia  

Science Journals Connector (OSTI)

Abstract Electron paramagnetic resonance (EPR) imaging using nitroxides as redox-sensitive probes is a powerful, noninvasive method that can be used under various physiological conditions to visualize changes in redox status that result from oxidative damage. Two blood–brain barrier-permeative nitroxides, 3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (HMP) and 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-yloxy (MCP), have been widely used as redox-sensitive probes in the brains of small animals, but their in vivo distribution and properties have not yet been analyzed in detail. In this study, a custom-made continuous-wave three-dimensional (3D) EPR imager was used to obtain 3D EPR images of mouse heads using MCP or HMP. This EPR imager made it possible to take 3D EPR images reconstructed from data from 181 projections acquired every 60 s. Using this improved EPR imager and magnetic resonance imaging, the distribution and reduction time courses of HMP and MCP were examined in mouse heads. EPR images of living mice revealed that HMP and MCP have different distributions and different time courses for entering the brain. Based on the pharmacokinetics of the reduction reactions of HMP and MCP in the mouse head, the half-lives of HMP and MCP were clearly and accurately mapped pixel by pixel. An ischemic mouse model was prepared, and the half-life of MCP was mapped in the mouse head. Compared to the half-life in control mice, the half-life of MCP in the ischemic model mouse brain was significantly increased, suggesting a shift in the redox balance. This in vivo EPR imaging method using BBB-permeative MCP is a useful noninvasive method for assessing changes in the redox status in mouse brains under oxidative stress.

Miho C. Emoto; Hideo Sato-Akaba; Hiroshi Hirata; Hirotada G. Fujii

2014-01-01T23:59:59.000Z

111

Differential effects of triclosan on the activation of mouse and human peroxisome proliferator-activated receptor alpha  

Science Journals Connector (OSTI)

Abstract Triclosan is an anti-bacterial agent used in many personal care products, household items, medical devices, and clinical settings. Liver tumors occur in mice exposed to triclosan, a response attributed to peroxisome proliferator-activated receptor alpha (PPAR?) activation; however, the effects of triclosan on mouse and human PPAR? have not been fully evaluated. We compared the effects of triclosan on mouse and human PPAR? using PPAR? reporter assays and on downstream events of PPAR? activation using mouse hepatoma Hepa1c1c7 cells and human hepatoma HepG2 cells. PPAR? transcriptional activity was increased by triclosan in a mouse PPAR? reporter assay and decreased in a human PPAR? reporter assay. Concentrations of triclosan inhibiting 50% cell growth were similar in both human and mouse hepatoma cells. Western blotting analysis showed that triclosan increased acyl-coenzyme A oxidase (ACOX1), a PPAR? target, in Hepa1c1c7 cells but decreased the level in HepG2 cells. Treatment of Hepa1c1c7 cells with triclosan enhanced DNA synthesis and suppressed transforming growth factor beta-mediated apoptosis. This did not occur in HepG2 cells. These data demonstrate that triclosan had similar cytotoxicity in Hepa1c1c7 and HepG2 cells, but differential effects on the activation of PPAR?, the expression of ACOX1, and downstream events including DNA synthesis and apoptosis.

Yuanfeng Wu; Qiangen Wu; Frederick A. Beland; Peter Ge; Mugimane G. Manjanatha; Jia-Long Fang

2014-01-01T23:59:59.000Z

112

Application of the optically stimulated luminescence (OSL) technique for mouse dosimetry in micro-CT imaging  

SciTech Connect (OSTI)

Purpose: Micro-CT is considered to be a powerful tool to investigate various models of disease on anesthetized animals. In longitudinal studies, the radiation dose delivered by the micro-CT to the same animal is a major concern as it could potentially induce spurious effects in experimental results. Optically stimulated luminescence dosimeters (OSLDs) are a relatively new kind of detector used in radiation dosimetry for medical applications. The aim of this work was to assess the dose delivered by the CT component of a micro-SPECT (single-photon emission computed tomography)/CT camera during a typical whole-body mouse study, using commercially available OSLDs based on Al{sub 2}O{sub 3}:C crystals.Methods: CTDI (computed tomography dose index) was measured in micro-CT with a properly calibrated pencil ionization chamber using a rat-like phantom (60 mm in diameter) and a mouse-like phantom (30 mm in diameter). OSLDs were checked for reproducibility and linearity in the range of doses delivered by the micro-CT. Dose measurements obtained with OSLDs were compared to those of the ionization chamber to correct for the radiation quality dependence of OSLDs in the low-kV range. Doses to tissue were then investigated in phantoms and cadavers. A 30 mm diameter phantom, specifically designed to insert OSLDs, was used to assess radiation dose over a typical whole-body mouse imaging study. Eighteen healthy female BALB/c mice weighing 27.1 ± 0.8 g (1 SD) were euthanized for small animal measurements. OLSDs were placed externally or implanted internally in nine different locations by an experienced animal technician. Five commonly used micro-CT protocols were investigated.Results: CTDI measurements were between 78.0 ± 2.1 and 110.7 ± 3.0 mGy for the rat-like phantom and between 169.3 ± 4.6 and 203.6 ± 5.5 mGy for the mouse-like phantom. On average, the displayed CTDI at the operator console was underestimated by 1.19 for the rat-like phantom and 2.36 for the mouse-like phantom. OSLDs exhibited a reproducibility of 2.4% and good linearity was found between 60 and 450 mGy. The energy scaling factor was calculated to be between 1.80 ± 0.16 and 1.86 ± 0.16, depending on protocol used. In phantoms, mean doses to tissue over a whole-body CT examination were ranging from 186.4 ± 7.6 to 234.9 ± 7.1 mGy. In mice, mean doses to tissue in the mouse trunk (thorax, abdomen, pelvis, and flanks) were between 213.0 ± 17.0 and 251.2 ± 13.4 mGy. Skin doses (3 OSLDs) were much higher with average doses between 350.6 ± 25.3 and 432.5 ± 34.1 mGy. The dose delivered during a topogram was found to be below 10 mGy. Use of the multimouse bed of the system gave a significantly 20%–40% lower dose per animal (p < 0.05).Conclusions: Absorbed doses in micro-CT were found to be relatively high. In micro-SPECT/CT imaging, the micro-CT unit is mainly used to produce a localization frame. As a result, users should pay attention to adjustable CT parameters so as to minimize the radiation dose and avoid any adverse radiation effects which may interfere with biological parameters studied.

Vrigneaud, Jean-Marc; Courteau, Alan; Oudot, Alexandra; Collin, Bertrand [Department of Nuclear Medicine, Centre Georges-François Leclerc, 1 rue Professeur Marion, Dijon 21079 Cedex (France)] [Department of Nuclear Medicine, Centre Georges-François Leclerc, 1 rue Professeur Marion, Dijon 21079 Cedex (France); Ranouil, Julien [Landauer Europe, 33 avenue du Général Leclerc, Fontenay-aux-Roses 92266 Cedex (France)] [Landauer Europe, 33 avenue du Général Leclerc, Fontenay-aux-Roses 92266 Cedex (France); Morgand, Loïc; Raguin, Olivier [Oncodesign, 20 rue Jean Mazen, Dijon 21076 Cedex (France)] [Oncodesign, 20 rue Jean Mazen, Dijon 21076 Cedex (France); Walker, Paul [LE2i CNRS UMR 5158, Faculty of Medicine, BP 87900, 21079 Dijon Cedex (France)] [LE2i CNRS UMR 5158, Faculty of Medicine, BP 87900, 21079 Dijon Cedex (France); Brunotte, François [Department of Nuclear Medicine, Centre Georges-François Leclerc, 1 rue Professeur Marion, Dijon 21079 Cedex, France and LE2i CNRS UMR 5158, Faculty of Medicine, BP 87900, 21079 Dijon Cedex (France)] [Department of Nuclear Medicine, Centre Georges-François Leclerc, 1 rue Professeur Marion, Dijon 21079 Cedex, France and LE2i CNRS UMR 5158, Faculty of Medicine, BP 87900, 21079 Dijon Cedex (France)

2013-12-15T23:59:59.000Z

113

Proteome and Transcriptome Profiles of a Her2/Neu-driven Mouse Model of Breast Cancer  

SciTech Connect (OSTI)

In recent years, mouse models have proven to be invaluable in expanding our understanding of cancer biology. We have amassed a tremendous amount of proteomics and transcriptomics data profiling blood and tissues from a Her2-driven mouse model of breast cancer that closely recapitulates the pathology and natural history of human breast cancer. The purpose of this report is to make all of these data publicly available in raw and processed forms, as a resource to the community. Importantly, high quality biospecimens from this same mouse model are freely available through a sample repository that we established, so researchers can readily obtain samples to test biological hypotheses without the need of breeding animals and collecting biospecimens. Specifically, six proteomics and six transcriptomics datasets are available, with the former encompassing 841 liquid chromatography-tandem mass spectrometry (LC-MS/MS) experiments of both plasma and tissue samples, and the latter including 255 individual microarray analyses of five different tissue types (thymus, spleen, liver, blood cells, and breast ± laser capture microdissection). A total of 18,880 unique peptides were identified with a PeptideProphet error rate ?1%, with 3884 non-redundant protein groups identified in five plasma datasets, and 1659 non-redundant protein groups in a tissue dataset (4977 non-redundant protein groups in total). We anticipate that these data will be of use to the community for software tool development, investigations of analytical variation in MS/MS data, development of quality control tools (multiple technical replicates are provided for a subset of the data), empirical selection of proteotypic peptides for multiple reaction monitoring mass spectrometry, and for advancing our understanding of cancer biology.

Schoenherr, Regine M.; Kelly-Spratt, Karen S.; Lin, Chen Wei; Whiteaker, Jeffrey R.; Liu, Tao; Holzman, Ted; Coleman, Ilsa; Feng, Li-Chia; Lorentzen, Travis D.; Krasnoselsky, Alexei L.; Wang, Pei; Liu, Yan; Gurley, Kay E.; Amon, Lynn M.; Schepmoes, Athena A.; Moore, Ronald J.; Camp, David G.; Chodosh, Lewis A.; Smith, Richard D.; Nelson, Peter S.; McIntosh, Martin; Kemp, Christopher; Paulovich, Amanda G.

2011-04-01T23:59:59.000Z

114

Sertad1 encodes a novel transcriptional co-activator of SMAD1 in mouse embryonic hearts  

SciTech Connect (OSTI)

Highlights: •SERTAD1 interacts with SMAD1. •Sertad1 is expressed in mouse embryonic hearts. •SERTAD1 is localized in both cytoplasm and nucleus of cardiomyocytes. •SERTAD1 enhances expression of BMP target cardiogenic genes as a SMAD1 co-activator. -- Abstract: Despite considerable advances in surgical repairing procedures, congenital heart diseases (CHDs) remain the leading noninfectious cause of infant morbidity and mortality. Understanding the molecular/genetic mechanisms underlying normal cardiogenesis will provide essential information for the development of novel diagnostic and therapeutic strategies against CHDs. BMP signaling plays complex roles in multiple cardiogenic processes in mammals. SMAD1 is a canonical nuclear mediator of BMP signaling, the activity of which is critically regulated through its interaction partners. We screened a mouse embryonic heart yeast two-hybrid library using Smad1 as bait and identified SERTAD1 as a novel interaction partner of SMAD1. SERTAD1 contains multiple potential functional domains, including two partially overlapping transactivation domains at the C terminus. The SERTAD1-SMAD1 interaction in vitro and in mammalian cells was further confirmed through biochemical assays. The expression of Sertad1 in developing hearts was demonstrated using RT-PCR, western blotting and in situ hybridization analyses. We also showed that SERTAD1 was localized in both the cytoplasm and nucleus of immortalized cardiomyocytes and primary embryonic cardiomyocyte cultures. The overexpression of SERTAD1 in cardiomyocytes not only enhanced the activity of two BMP reporters in a dose-dependent manner but also increased the expression of several known BMP/SMAD regulatory targets. Therefore, these data suggest that SERTAD1 acts as a SMAD1 transcriptional co-activator to promote the expression of BMP target genes during mouse cardiogenesis.

Peng, Yin [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States)] [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Zhao, Shaomin [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States) [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States); School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069 (China); Song, Langying [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States)] [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States); Wang, Manyuan [School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069 (China)] [School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069 (China); Jiao, Kai, E-mail: kjiao@uab.edu [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States)] [Department of Genetics, The University of Alabama at Birmingham, Birmingham, AL 35294 (United States)

2013-11-29T23:59:59.000Z

115

Comparative promoter activities of three endogenous copies of mouse mammary tumor virus  

E-Print Network [OSTI]

to 12 CC ~ o c 0 tu x MMTV M13 pLC1 ?(, a~ CAT pBR322 0/ I SV40 Figure 5. Diagram of pLC1. The plasmid pLC1 (Toohey et al. , 1986) contains an Intact LTR obtained from the C3H provirus as a Pst I restriction fragment. The plasmid also...COMPARATIVE PROMOTER ACTIVITIES OF THREE ENDOGENOUS COPIES OF MOUSE MAMMARY TUMOR VIRUS A Thesis by LAURA REID VILANDER Submitted to the Graduate College of Texas A&M University in partial fulfilment of the requirements for the degree...

Vilander, Laura Reid

1987-01-01T23:59:59.000Z

116

The genetic control of avascular area in mouse oxygen-induced retinopathy  

E-Print Network [OSTI]

The genetic control of avascular area in mouse oxygen-induced retinopathy Bliss E. O’Bryhim,1,2 Jeff Radel,1,2,3 Stuart J. Macdonald,4 R.C. Andrew Symons1,2 1Department of Ophthalmology, University of Kansas Medical Center, Kansas City, KS; 2... exposure to hyperoxia, as well as quantitative trait loci on chromosomes 7 and 9 that modify susceptibility to OIR. Retinopathy of prematurity (ROP) is a leading cause of vision loss in children in both developed and developing countries [1–3]. ROP is a...

O'Bryhim, Bliss; Radel, Jeff; Macdonald, Stuart J.; Symons, R. C. Andrew

2012-02-08T23:59:59.000Z

117

Pointright: a system to redirect mouse and keyboard control among multiple machines  

DOE Patents [OSTI]

The present invention provides a software system, PointRight, that allows for smooth and effortless control of pointing and input devices among multiple displays. With PointRight, a single free-floating mouse and keyboard can be used to control multiple screens. When the cursor reaches the edge of a screen it seamlessly moves to the adjacent screen and keyboard control is simultaneously redirected to the appropriate machine. Laptops may also redirect their keyboard and pointing device, and multiple pointers are supported simultaneously. The system automatically reconfigures itself as displays go on, go off, or change the machine they display.

Johanson, Bradley E. (Palo Alto, CA); Winograd, Terry A. (Stanford, CA); Hutchins, Gregory M. (Mountain View, CA)

2008-09-30T23:59:59.000Z

118

Embryotoxic and histopathologic effects of aflatoxin B1 during early pregnancy in the mouse  

E-Print Network [OSTI]

During Early Pregnancy in the Mouse, (August 1990) Ying Jia-Hsu, M. D. , Capital Institute of Medicine Co-chairs of Advisory Committee: Dr. T, Rick Irvin Dr. John E. Martin The in vivo embryotoxicity of aflatoxin Bl (AFB1) on mice..., and with histopathologic changes that occur as a result of exposure to AFB1 at a single maximum tolerate dose on gestation day 2 or gestation day 4 were characterized, and the correlation of the embryotoxic and histopathologic effects of AFB1 on pregnant mice...

Jia-Hsu, Ying

2012-06-07T23:59:59.000Z

119

Stimulative Effects of Estrogens on Tumor Growth and 5?-Steroid Production in a Mouse Leydig Cell Tumor Line (T 124958-R)  

Science Journals Connector (OSTI)

...cells were harvested by a rubber policeman at the indicated...conditions used, the rate of production of 5 -or 17/3-hydroxy...FEBRUARY 1986 574 5 -ANDROGEN PRODUCTION IN MOUSE LEYDIG CELL TUMOR...FEBRUARY 1986 575 5a-ANDROGEN PRODUCTION IN MOUSE LEYDIG CELL TUMOR...

Shinzaburo Noguchi; Yasuko Nishizawa; Daishiro Takatsuka; Bunzo Sato; and Keishi Matsumoto

1986-02-01T23:59:59.000Z

120

Effect of Preirradiation of the Tumor Bed on the Relative Vascular Space of Mouse Gastric Adenocarcinoma 328 and Mammary Adenocarcinoma CA755  

Science Journals Connector (OSTI)

...viable portions of tumors of the MTG-B strain show an accelerating...not reflect the fundamental processes occurring in those areas of...Growth and Cell Loss in the MTG-B Mouse Mammary Carcinoma...of Mouse Mammary Tumor Strain MTG-B. Proc. Soc. Exptl. Biol...

Randy Jirtle and Kelly H. Clifton

1973-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Natural history and karyology of the Yucatán vesper mouse, Otonyctomys hatti  

E-Print Network [OSTI]

Seventeen specimens of the rare Yucatán vesper mouse, Otonyctomys hatti, are now known from Belize, Guatemala, and the Mexican states of Campeche, Quintana Roo, and Yucatán. We herein report a second specimen of O. hatti, from Belize, extending the known geographic range of the species 95 km to the southeast in the country. This is the first location at which O. hatti has been taken sympatrically with the Central American vesper mouse, Nyctomys sumichrasti. We also report data on three additional specimens of O. hatti from Campeche. Nyctomys and Otonyctomys share similar habits and habitat requirements, and might compete where they overlap. However, the distribution of O. hatti corresponds closely to that of other Yucatán endemics, and the distinct distributions of the two genera probably reflects biogeographic history and different habitat requirements, rather than result from direct competition. The karyotype of O. hatti is 2n = 50, FN = 58. Although superficially similar, it differs in important respects with the karyotypes reported for N. sumichrasti....Resumen--Diescisiete especímenes del ratón Otonyctomys hatti, especie rara de Yucatán, son ahora conocidos de Belice, Guatemala y los Estados de Campeche, Quintana Roo y Yucatán. Aquí, informamos de un segundo especímen de O. hatti de Belice...

Genoways, Hugh H.; Timm, Robert M.; Engstrom, Mark D.

2005-01-01T23:59:59.000Z

122

Expression of HIV-Tat protein is associated with learning and memory deficits in the mouse  

Science Journals Connector (OSTI)

HIV-Tat protein has been implicated in the pathogenesis of HIV-1 neurological complications (i.e., neuroAIDS), but direct demonstrations of the effects of Tat on behavior are limited. GT-tg mice with a doxycycline (Dox)-inducible and brain-selective tat gene coding for Tat protein were used to test the hypothesis that the activity of Tat in brain is sufficient to impair learning and memory processes. Western blot analysis of GT-tg mouse brains demonstrated an increase in Tat antibody labeling that seemed to be dependent on the dose and duration of Dox pretreatment. Dox-treated GT-tg mice tested in the Barnes maze demonstrated longer latencies to find an escape hole and displayed deficits in probe trial performance versus uninduced GT-tg littermates, suggesting Tat-induced impairments of spatial learning and memory. Reversal learning was also impaired in Tat-induced mice. Tat-induced mice additionally demonstrated long-lasting (up to one month) deficiencies in novel object recognition learning and memory performance. Furthermore, novel object recognition impairment was dependent on the dose and duration of Dox exposure, suggesting that Tat exposure progressively mediated deficits. These experiments provide evidence that Tat protein expression is sufficient to mediate cognitive abnormalities seen in HIV-infected individuals. Moreover, the genetically engineered GT-tg mouse may be useful for improving our understanding of the neurological underpinnings of neuroAIDS-related behaviors.

Amanda N. Carey; Elizabeth I. Sypek; Harminder D. Singh; Marc J. Kaufman; Jay P. McLaughlin

2012-01-01T23:59:59.000Z

123

Manuscript Version 6. Official copy "Mouse" in Berkshire Encyclopedia of Human-Computer Interaction, W.S. Bainbridge (ed). 2004 by Berkshire Publishing Group. http://www.berkshirepublishing.com/brw/BerkProd.asp?projID=29#  

E-Print Network [OSTI]

interface and many other innovations. There are various possible alternatives to the mouse, including touch is that for interacting with graphical user interfaces (GUI) on desktop computers, the mouse serves most users quite well two orthogonal optical encoders for two dimensional movements. Figure 2 shows the inside of a mouse

Zhai, Shumin

124

15 May 09 52MUniversity College London 4.4.11 rack 1Mouse Health Monitoring Report  

E-Print Network [OSTI]

15 May 09 52MUniversity College London 4.4.11 rack 1Mouse Test Date Health Monitoring ReportServ@harlan.com #12;15 May 09 52MUniversity College London 4.4.11 rack 1BMouse Test Date Health Monitoring Report

Richardson, William D.

125

Sulforaphane induces phase II detoxication enzymes in mouse skin and prevents mutagenesis induced by a mustard gas analog  

SciTech Connect (OSTI)

Mustard gas, used in chemical warfare since 1917, is a mutagenic and carcinogenic agent that produces severe dermal lesions for which there are no effective therapeutics; it is currently seen as a potential terrorist threat to civilian populations. Sulforaphane, found in cruciferous vegetables, is known to induce enzymes that detoxify compounds such as the sulfur mustards that react through electrophilic intermediates. Here, we observe that a single topical treatment with sulforaphane induces mouse epidermal levels of the regulatory subunit of glutamate-cysteine ligase, the rate-limiting enzyme in glutathione biosynthesis, and also increases epidermal levels of reduced glutathione. Furthermore, a glutathione S-transferase, GSTA4, is also induced in mouse skin by sulforaphane. In an in vivo model in which mice are given a single mutagenic application of the sulfur mustard analog 2-(chloroethyl) ethyl sulfide (CEES), we now show that therapeutic treatment with sulforaphane abolishes the CEES-induced increase in mutation frequency in the skin, measured four days after exposure. Sulforaphane, a natural product currently in clinical trials, shows promise as an effective therapeutic against mustard gas. -- Highlights: ? Sulforaphane induces increased levels of glutathione in mouse skin. ? Sulforaphane induces increased levels of GSTA4 in mouse skin. ? Sulforaphane, applied after CEES-treatment, completely abolishes CEES-mutagenesis. ? The therapeutic effect may suggest a long biological half-life for CEES in vivo.

Abel, E.L. [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States)] [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States); Boulware, S. [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States)] [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States); Fields, T.; McIvor, E.; Powell, K.L. [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States)] [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States); DiGiovanni, J.; Vasquez, K.M. [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States)] [Division of Pharmacy and Toxicology, College of Pharmacy, The University of Texas at Austin, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd., Austin, TX 78723 (United States); MacLeod, M.C., E-mail: mcmacleod@mdanderson.org [Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park, Smithville, TX 78957 (United States)

2013-02-01T23:59:59.000Z

126

Intervening sequences divide the gene for the constant region of mouse immunoglobulin mu chains into segments, each encoding a domain  

Science Journals Connector (OSTI)

...mouse Au constant region (25). We prepared restriction frag- merits of the cloned cDNA as probes for specific portions of the V...provided a 3'-Cg specific probe. Fragments were labeled by nick translation (31). E L H76 NW *0 -12.5 -10 ,W - 7...

N M Gough; D J Kemp; B M Tyler; J M Adams; S Cory

1980-01-01T23:59:59.000Z

127

``In-bone'' Utricle CulturesVA Simplified, Atraumatic Technique for In Situ Cultures of the Adult Mouse  

E-Print Network [OSTI]

effectively in stud- ies of hair cell regeneration and hair cell death, as well as various agents that protect and protection. Background: The current in vitro technique for studying hair cells of the mature mouse utricle to the protective drug tacrine resulted in sig- nificant protection against neomycin (p G 0.05, 3-way analysis

Rubel, Edwin

128

3D segmentation of mouse organs from MR images using deformable simplex mesh models G. Hamarneh1  

E-Print Network [OSTI]

3D segmentation of mouse organs from MR images using deformable simplex mesh models G. Hamarneh1 , H. Delingette2 , M. Henkelman1 1 Hospital for Sick Children, Toronto, ON, Canada, 2 INRIA brains and kidneys from MR images. Algorithmic details and 3D segmentation results are presented

Hamarneh, Ghassan

129

Cell, Vol. 69, 1097-l 106, June 26, 1992, Copyright 0 1992 by Cell Press Gastrulation in the Mouse  

E-Print Network [OSTI]

the three germ layers- ectoderm, mescderm, and endoderm-are generated dur- ing animal development. After that glves rise to the head process. Treatment of early mouse embryos wlth activin results in goosecoid m gastrulation is completed, most vertebrate embryos have a remarkably similar body plan (von Baer, 1828

Blumberg, Bruce

130

High concentrations of morphine sensitize and activate mouse dorsal root ganglia via TRPV1 and TRPA1 receptors  

E-Print Network [OSTI]

and sensitized the release evoked by heat or the TRPA1 agonist acrolein. Morphine activated HEK293t cells transfected with TRPV1 or TRPA1. Activation of C57BL/6 mouse dorsal root ganglion neurons in culture was investigated with calcium imaging. Morphine induced...

Forster, Alexander B; Reeh, Peter; Messlinger, Karl; Fischer, Michael J M

2009-04-16T23:59:59.000Z

131

Whole Slide Image Analysis Quantification using Aperio Digital Imaging in a Mouse Lung Metastasis Ronne L. Surface2  

E-Print Network [OSTI]

Whole Slide Image Analysis Quantification using Aperio Digital Imaging in a Mouse Lung Metastasis, Indiana University School of Medicine Digital whole slide imaging is the technique of digitizing a microscope slide at the highest resolution to produce a "digital virtual microscope slide". This digital

Zhou, Yaoqi

132

Mislocalization and Degradation of Human P23H-Rhodopsin-GFP in a Knockin Mouse Model of  

E-Print Network [OSTI]

in the inner segments of the frog rod cells, but does not form aggregates.9 Retention of P23HMislocalization and Degradation of Human P23H-Rhodopsin-GFP in a Knockin Mouse Model of Retinitis the effects of treatments on degradation and mislo- calization of proline-to-histidine change at codon 23 (P23

Wensel, Theodore G.

133

Bioactivation of food genotoxicants 5-hydroxymethylfurfural and furfuryl alcohol by sulfotransferases from human, mouse and rat: a comparative study  

Science Journals Connector (OSTI)

5-Hydroxymethylfurfural (HMF) and furfuryl alcohol (FFA)...k cat/K M) of HMF sulfoconjugation of human SULT1A1 (13.7 s?1 M?1), mouse Sult1a1 (15.8 s?1 M?1) and 1d1 (4.8 s?1 M?1) and r...

Benjamin Sachse; Walter Meinl; Yasmin Sommer; Hansruedi Glatt…

2014-11-01T23:59:59.000Z

134

Low-Dose Ionizing Radiation Alters the Epigenome of the Avy Mouse  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Ionizing Radiation Alters the Epigenome of the A Ionizing Radiation Alters the Epigenome of the A vy Mouse Autumn Bernal 1,2,3 , Dale Huang 1 , Yue Li 4 , Dana Dolinoy 5 , and Randy Jirtle 1 Department of Radiation Oncology 1 , University Program in Genetics and Genomic 2 , Integrated Toxicology & Environmental Health Program 3 , Department of Community and Family Medicine 4 , Duke University Medical Center, Durham, NC, USA, Department of Environmental and Health Sciences, University of Michigan, Ann Arbor, MI, USA 4 Background: Humans have evolved and thrived amidst constant low-dose (0-10 cGy) background radiation exposure from natural sources. Currently, however, the frequency of exposures to low doses of radiation is increasing due to man-made sources such as diagnostic imaging and nuclear power. This increased exposure has led to concerns amongst the general public and the government about the

135

A simple, low-cost, data logging pendulum built from a computer mouse  

SciTech Connect (OSTI)

Lessons and homework problems involving a pendulum are often a big part of introductory physics classes and laboratory courses from high school to undergraduate levels. Although laboratory equipment for pendulum experiments is commercially available, it is often expensive and may not be affordable for teachers on fixed budgets, particularly in developing countries. We present a low-cost, easy-to-build rotary sensor pendulum using the existing hardware in a ball-type computer mouse. We demonstrate how this apparatus may be used to measure both the frequency and coefficient of damping of a simple physical pendulum. This easily constructed laboratory equipment makes it possible for all students to have hands-on experience with one of the most important simple physical systems.

Gintautas, Vadas [Los Alamos National Laboratory; Hubler, Alfred [UIUC

2009-01-01T23:59:59.000Z

136

Purification and characterization of mouse DNA polymerase ? devoid of primase activity  

Science Journals Connector (OSTI)

A simple method was developed for the isolation of primase-free DNA polymerase-? from the DNA polymerase-?-primase complex of mouse FM3A cells. The polymerase was separated from primase submits by chromatography on a single-stranded DNA-cellulose column in the presence of 50% etylene glycol. The primase-free DNA polymerase-? contained two polypeptides with molecular masses of 180000 and 68000. Analysis of the DNA products with poly(dA)-oligo(dT)10 as template-primer revealed that both primase-free DNA polymerase-? and the DNA polymerase-?-primase complex predominantly synthesized short DNA with less than 30 nucleotides, but that the DNA polymerase-?-primase complex also synthesized some longer DNA with more than 300–400 nucleotides.

Ritsuko Takada-Takayama; Masashi Suzuki; Takemi Enomoto; Fumio Hanaoka; Michio Ui

1990-01-01T23:59:59.000Z

137

Triclosan induces Fas receptor-dependent apoptosis in mouse neocortical neurons in vitro  

Science Journals Connector (OSTI)

Abstract Triclosan (TCS) is a commonly used antimicrobial agent in personal care and sanitizing products, as well as in household items. Numerous studies have demonstrated the presence of TCS in various human tissues. Several studies have reported the accumulation of TCS in fish and human brain tissue. The aim of the present study was to investigate the effect of TCS on apoptosis in mouse neocortical neurons after 7 days of culture in vitro following 3, 6 and 24 h of exposure. To explore the mechanism underlying the effects of TCS in neurons, we studied the activation and protein expression of the Fas receptor (FasR) and caspase-8, caspase-9 and caspase-3, as well as DNA fragmentation in TCS-treated cells. Cultures of neocortical neurons were prepared from Swiss mouse embryos on day 15/16 of gestation. The cells were cultured in phenol red-free Neurobasal medium with B27 and glutamine. The cultures were treated with concentrations of TCS ranging from 1 nM to 100 ?M for 3, 6 and 24 h. The level of lactate dehydrogenase (LDH) was measured in the culture medium to exclude the cytotoxic concentrations. The cytotoxic effects were only observed when the highest concentrations of TCS were used (50 and 100 ?M). To study apoptosis, the activities of caspase-8, caspase-9 and caspase-3 were measured, and DNA fragmentation was evaluated. Our results are the first time to demonstrate that TCS can induce an apoptotic process in neocortical neurons in vitro. The data demonstrated that TCS caused caspase-3 activation, DNA fragmentation and apoptotic body formation. Non-cytotoxic concentrations of TCS activated the extrinsic apoptotic signaling pathway, which is dependent on FasR and caspase-8 activation. However, it is also possible that TCS may activate the intrinsic apoptotic pathway after long-term exposure. Therefore, further studies on the mechanism underlying the effects of TCS on the nervous system are needed.

K.A. Szychowski; A.M. Sitarz; A.K. Wojtowicz

2015-01-01T23:59:59.000Z

138

A mouse model links asthma susceptibility to prenatal exposure to diesel exhaust  

Science Journals Connector (OSTI)

Background Most asthma begins in the first years of life. This early onset cannot be attributed merely to genetic factors because the prevalence of asthma is increasing. Epidemiologic studies have indicated roles for prenatal and early childhood exposures, including exposure to diesel exhaust. However, little is known about the mechanisms. This is largely due to a paucity of animal models. Objective We aimed to develop a mouse model of asthma susceptibility through prenatal exposure to diesel exhaust. Methods Pregnant C57BL/6 female mice were given repeated intranasal applications of diesel exhaust particles (DEPs) or PBS. Offspring underwent suboptimal immunization and challenge with ovalbumin (OVA) or received PBS. Pups were examined for features of asthma; lung and liver tissues were analyzed for transcription of DEP-regulated genes. Results Offspring of mice exposed to \\{DEPs\\} were hypersensitive to OVA, as indicated by airway inflammation and hyperresponsiveness, increased serum OVA-specific IgE levels, and increased pulmonary and systemic TH2 and TH17 cytokine levels. These cytokines were primarily produced by natural killer (NK) cells. Antibody-mediated depletion of NK cells prevented airway inflammation. Asthma susceptibility was associated with increased transcription of genes known to be specifically regulated by the aryl hydrocarbon receptor and oxidative stress. Features of asthma were either marginal or absent in OVA-treated pups of PBS-exposed mice. Conclusion We created a mouse model that linked maternal exposure to \\{DEPs\\} with asthma susceptibility in offspring. Development of asthma was dependent on NK cells and associated with increased transcription from aryl hydrocarbon receptor– and oxidative stress–regulated genes.

Sarah Manners; Rafeul Alam; David A. Schwartz; Magdalena M. Gorska

2014-01-01T23:59:59.000Z

139

Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe  

E-Print Network [OSTI]

We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide ...

McQuade, Lindsey E.

140

A mutation in the envelope protein fusion loop attenuates mouse neuroinvasiveness of the NY99 strain of West Nile virus  

SciTech Connect (OSTI)

Substitutions were engineered individually and in combinations at the fusion loop, receptor-binding domain and a stem-helix structure of the envelope protein of a West Nile virus strain, NY99, and their effects on mouse virulence and presentation of epitopes recognized by monoclonal antibodies (MAbs) were assessed. A single substitution within the fusion loop (L107F) attenuated mouse neuroinvasiveness of NY99. No substitutions attenuated NY99 neurovirulence. The L107F mutation also abolished binding of a non-neutralizing MAb, 3D9, whose epitope had not been previously identified. MAb 3D9 was subsequently shown to be broadly cross-reactive with other flaviviruses, consistent with binding near the highly conserved fusion loop.

Zhang Shuliu [Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States); Li Li; Woodson, Sara E. [Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States); Huang, Claire Y.-H.; Kinney, Richard M. [Arboviral Diseases Branch, Division of Vector-Borne Infectious Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Fort Collins, CO (United States); Barrett, Alan D.T. [Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Institute for Human Infections and Immunity, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States); Beasley, David W.C. [Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Sealy Center for Vaccine Development, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]|[Institute for Human Infections and Immunity, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555 (United States)]. E-mail: d.beasley@utmb.edu

2006-09-15T23:59:59.000Z

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141

Tissue distribution of the dystrophin-related gene product and expression in the mdx and dy mouse  

SciTech Connect (OSTI)

The authors have previously reported a dystrophin-related locus (DMDL for Duchenne muscular dystrophy-like) on human chromosome 6 that maps close to the dy mutation on mouse chromosome 10. Here they show that this gene is expressed in a wide range of tissues at varying levels. The transcript is particularly abundant in several human fetal tissues, including heart, placenta, and intestine. Studies with antisera raised against a DMDL fusion protein identify a 400,000 M{sub r} protein in all mouse tissues tested, including those of mdx and dy mice. Unlike the dystrophin gene, the DMDL gene transcript is not differentially spliced at the 3{prime} end in either fetal muscle or brain.

Love, D.R.; Marsden, R.F.; Bloomfield, J.F.; Davies, K.E. (John Radcliffe Hospital, Oxford (England)); Morris, G.E.; Ellis, J.M. (North East Wales Inst., Deeside, Wales (England)); Fairbrother, U.; Edwards, Y.H. (Univ. College London (England)); Slater, C.P. (Newcastle General Hospital, Newcastle-upon-Tyne (England)); Parry, D.J. (Univ. of Ottawa, Ontario (Canada))

1991-04-15T23:59:59.000Z

142

Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer A transgenic mouse model expressing NSE-htau23 was used. Black-Right-Pointing-Pointer 2D-gel electrophoresis to analyze the cortex proteins of transgenic mice was used. Black-Right-Pointing-Pointer Differentially expressed spots in different stages of AD were identified. Black-Right-Pointing-Pointer GSTP1 and CAII were downregulated with the progression of AD. Black-Right-Pointing-Pointer SCRN1 and ATP6VE1 were up regulated and down regulated differentially. -- Abstract: Alzheimer's disease (AD) involves regionalized neuronal death, synaptic loss, and an accumulation of intracellular neurofibrillary tangles and extracellular senile plaques. Although there have been numerous studies on tau proteins and AD in various stages of neurodegenerative disease pathology, the relationship between tau and AD is not yet fully understood. A transgenic mouse model expressing neuron-specific enolase (NSE)-controlled human wild-type tau (NSE-htau23), which displays some of the typical Alzheimer-associated pathological features, was used to analyze the brain proteome associated with tau tangle deposition. Two-dimensional electrophoresis was performed to compare the cortex proteins of transgenic mice (6- and 12-month-old) with those of control mice. Differentially expressed spots in different stages of AD were identified with ESI-Q-TOF (electrospray ionization quadruple time-of-flight) mass spectrometry and liquid chromatography/tandem mass spectrometry. Among the identified proteins, glutathione S-transferase P 1 (GSTP1) and carbonic anhydrase II (CAII) were down-regulated with the progression of AD, and secerin-1 (SCRN1) and V-type proton ATPase subunit E 1 (ATP6VE1) were up-regulated only in the early stages, and down-regulated in the later stages of AD. The proteins, which were further confirmed by RT-PCR at the mRNA level and with western blotting at the protein level, are expected to be good candidates as drug targets for AD. The study of up- and down-regulation of proteins during the progression of AD helps to explain the mechanisms associated with neuronal degeneration in AD.

Chang, Seong-Hun; Jung, In-Soo; Han, Gi-Yeon; Kim, Nam-Hee; Kim, Hyun-Jung [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)] [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of); Kim, Chan-Wha, E-mail: cwkim@korea.ac.kr [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)] [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)

2013-01-11T23:59:59.000Z

143

Induction of chloride secretory currents across mouse ileal tissues by rotavirus enterotoxic peptide in different age mice  

E-Print Network [OSTI]

INDUCTION OF CHLORIDE SECRETORY CURRENTS ACROSS MOUSE ILEAL TISSUES BY A ROTAVIRUS ENTEROTOXIC PEPTIDE IN DIFFERENT AGE MICE A Thesis by VIRGINIA WATERS COX Submitted to the Office of Graduate Studies of Texas Ak M University in partial... by VIRGINIA WATERS COX Submitted to Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Approved as to style and content by: Judith M. Ball (Chair of Committee) Julian Lei os z (Member) Friedhelm...

Cox, Virginia Waters

2002-01-01T23:59:59.000Z

144

Effects of dietary eicosapentaenoic acid and docosahexaenoic acid on mouse T-lymphocyte function and diglyceride formation  

E-Print Network [OSTI]

. Edwards (Interim Department Head) December 1992 ABSTRACT Effects of Dietary Eicosapentaenoic Acid and Docosahexaenoic Acid on Mouse T-Lymphocyte Function and Diglyceride Formation. (December 1992) Kara Hosack Fowler, B. S. , Texas A&%M University..., coupled to CD3, a mediator which acts in stimulation to bind to phospholipase C (PLC) (10). Mitogens act on the TCR-CD3 complex to stimulate phospholipase C and hydrolysis of phosphatidylinositol (100). This results in diacylglycerol (DAG) and inositol...

Fowler, Kara Hosack

1992-01-01T23:59:59.000Z

145

Multi-walled carbon nanotube-induced gene expression in the mouse lung: Association with lung pathology  

SciTech Connect (OSTI)

Due to the fibrous shape and durability of multi-walled carbon nanotubes (MWCNT), concerns regarding their potential for producing environmental and human health risks, including carcinogenesis, have been raised. This study sought to investigate how previously identified lung cancer prognostic biomarkers and the related cancer signaling pathways are affected in the mouse lung following pharyngeal aspiration of well-dispersed MWCNT. A total of 63 identified lung cancer prognostic biomarker genes and major signaling biomarker genes were analyzed in mouse lungs (n = 80) exposed to 0, 10, 20, 40, or 80 {mu}g of MWCNT by pharyngeal aspiration at 7 and 56 days post-exposure using quantitative PCR assays. At 7 and 56 days post-exposure, a set of 7 genes and a set of 11 genes, respectively, showed differential expression in the lungs of mice exposed to MWCNT vs. the control group. Additionally, these significant genes could separate the control group from the treated group over the time series in a hierarchical gene clustering analysis. Furthermore, 4 genes from these two sets of significant genes, coiled-coil domain containing-99 (Ccdc99), muscle segment homeobox gene-2 (Msx2), nitric oxide synthase-2 (Nos2), and wingless-type inhibitory factor-1 (Wif1), showed significant mRNA expression perturbations at both time points. It was also found that the expression changes of these 4 overlapping genes at 7 days post-exposure were attenuated at 56 days post-exposure. Ingenuity Pathway Analysis (IPA) found that several carcinogenic-related signaling pathways and carcinogenesis itself were associated with both the 7 and 11 gene signatures. Taken together, this study identifies that MWCNT exposure affects a subset of lung cancer biomarkers in mouse lungs. - Research Highlights: > Multi-Walled Carbon Nanotubes affect lung cancer biomarkers in mouse lungs. > The results suggest potentially harmful effects of MWCNT exposure on human lungs. > The results could potentially be used for the medical surveillance of workers.

Pacurari, M. [Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506-9300 (United States); Qian, Y., E-mail: yaq2@cdc.gov [Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Porter, D.W.; Wolfarth, M. [Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Wan, Y.; Luo, D. [Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506-9300 (United States); Ding, M. [Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Castranova, V. [Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506-9300 (United States); Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505 (United States); Guo, N.L., E-mail: lguo@hsc.wvu.edu [Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506-9300 (United States); Department of Community Medicine, School of Medicine, West Virginia University, Morgantown, WV 26506-9190 (United States)

2011-08-15T23:59:59.000Z

146

A physiologically based pharmacokinetic model for atrazine and its main metabolites in the adult male C57BL/6 mouse  

SciTech Connect (OSTI)

Atrazine (ATR) is a chlorotriazine herbicide that is widely used and relatively persistent in the environment. In laboratory rodents, excessive exposure to ATR is detrimental to the reproductive, immune, and nervous systems. To better understand the toxicokinetics of ATR and to fill the need for a mouse model, a physiologically based pharmacokinetic (PBPK) model for ATR and its main chlorotriazine metabolites (Cl-TRIs) desethyl atrazine (DE), desisopropyl atrazine (DIP), and didealkyl atrazine (DACT) was developed for the adult male C57BL/6 mouse. Taking advantage of all relevant and recently made available mouse-specific data, a flow-limited PBPK model was constructed. The ATR and DACT sub-models included blood, brain, liver, kidney, richly and slowly perfused tissue compartments, as well as plasma protein binding and red blood cell binding, whereas the DE and DIP sub-models were constructed as simple five-compartment models. The model adequately simulated plasma levels of ATR and Cl-TRIs and urinary dosimetry of Cl-TRIs at four single oral dose levels (250, 125, 25, and 5 mg/kg). Additionally, the model adequately described the dose dependency of brain and liver ATR and DACT concentrations. Cumulative urinary DACT amounts were accurately predicted across a wide dose range, suggesting the model's potential use for extrapolation to human exposures by performing reverse dosimetry. The model was validated using previously reported data for plasma ATR and DACT in mice and rats. Overall, besides being the first mouse PBPK model for ATR and its Cl-TRIs, this model, by analogy, provides insights into tissue dosimetry for rats. The model could be used in tissue dosimetry prediction and as an aid in the exposure assessment to this widely used herbicide.

Lin Zhoumeng [Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602 (United States); Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602 (United States); Fisher, Jeffrey W. [Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602 (United States); Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602 (United States); Ross, Matthew K. [Center for Environmental Health Sciences, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762 (United States); Filipov, Nikolay M., E-mail: filipov@uga.edu [Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602 (United States); Interdisciplinary Toxicology Program, University of Georgia, Athens, GA 30602 (United States)

2011-02-15T23:59:59.000Z

147

A comparison of the metabolism of HEOD (dieldrin) in the CF1 mouse with that in the CFE rat  

Science Journals Connector (OSTI)

HEOD labelled with 14C was administered to CFE rats and CF1 mice and the excreted radioactivity was examined to characterize the various HEOD metabolites. In addition to the pentachloroketone and 9-hydroxy metabolites, the identities of two further metabolites of HEOD in the rat were established. These are a faecal metabolite, 6,7-trans-dihydroaldrindiol, and a urinary metabolite, the hexachlorohexahydromethanoindene-dicarboxylic acid, which may be formed by oxidation of the diol. Three of the rat metabolites, but not the pentachloroketone, were found in the excreta of the CF1 mouse. Where possible the quantity of radio-activity present as any given metabolite was determined. A sample of the new urinary metabolite of the rat was synthesized and shown to have an acute oral toxicity of >850 mg/kg in the CF1 mouse. Prior induction of enzyme activity appeared to affect the production of urinary metabolites of HEOD in the CFE rat but not in the CF1 mouse.

M.K. Baldwin; J. Robinson; D.V. Parke

1972-01-01T23:59:59.000Z

148

Differential gene expression in mouse liver associated with the hepatoprotective effect of clofibrate  

SciTech Connect (OSTI)

Pretreatment of mice with the peroxisome proliferator clofibrate (CFB) protects against acetaminophen (APAP)-induced hepatotoxicity. Previous studies have shown that activation of the nuclear peroxisome proliferator activated receptor-alpha (PPAR{alpha}) is required for this effect. The present study utilizes gene expression profile analysis to identify potential pathways contributing to PPAR{alpha}-mediated hepatoprotection. Gene expression profiles were compared between wild type and PPAR{alpha}-null mice pretreated with vehicle or CFB (500 mg/kg, i.p., daily for 10 days) and then challenged with APAP (400 mg/kg, p.o.). Total hepatic RNA was isolated 4 h after APAP treatment and hybridized to Affymetrix Mouse Genome MGU74 v2.0 GeneChips. Gene expression analysis was performed utilizing GeneSpring (registered) software. Our analysis identified 53 genes of interest including vanin-1, cell cycle regulators, lipid-metabolizing enzymes, and aldehyde dehydrogenase 2, an acetaminophen binding protein. Vanin-1 could be important for CFB-mediated hepatoprotection because this protein is involved in the synthesis of cysteamine and cystamine. These are potent antioxidants capable of ameliorating APAP toxicity in rodents and humans. HPLC-ESI/MS/MS analysis of liver extracts indicates that enhanced vanin-1 gene expression results in elevated cystamine levels, which could be mechanistically associated with CFB-mediated hepatoprotection.

Moffit, Jeffrey S. [University of Connecticut, Department of Pharmaceutical Sciences, Storrs, CT (United States); Koza-Taylor, Petra H. [Pfizer, Inc., Groton Laboratories, Molecular and Investigative Toxicology, Groton, CT (United States); Holland, Ricky D. [National Center for Toxicological Research, Division of Systems Toxicology, Jefferson, AR (United States); Thibodeau, Michael S. [University of Connecticut, Department of Pharmaceutical Sciences, Storrs, CT (United States); Beger, Richard D. [National Center for Toxicological Research, Division of Systems Toxicology, Jefferson, AR (United States); Lawton, Michael P. [Pfizer, Inc., Groton Laboratories, Molecular and Investigative Toxicology, Groton, CT (United States); Manautou, Jose E. [University of Connecticut, Department of Pharmaceutical Sciences, Storrs, CT (United States)]. E-mail: jose.manautou@uconn.edu

2007-07-15T23:59:59.000Z

149

Alteration of mouse oocyte quality after a subchronic exposure to depleted Uranium  

Science Journals Connector (OSTI)

Gametes and embryo tissues are known to represent a sensitive target to environmental toxicants exposure. Oocyte quality can impact subsequent developmental competence, pregnancy course and even adult health. The major health concern from depleted uranium (DU) is mainly centred on its chemotoxic properties as a heavy metal. Little attention was paid to the impact of uranium on female gamete quality. The aim of this research was to evaluate the effect of DU on mouse oocyte quality after 49 days of subchronic contamination in drinking water and to correlate the observed effects with the amount of DU accumulated in organs. Four different DU concentrations were investigated: 0 (control), 10 (DU10), 20 (DU20) and 40 mg L?1 (DU40). DU did not influence the intensity of ovulation but affected oocyte quality. The proportion of healthy oocytes was reduced by half (P < 0.001) from 20 mg L?1 compared with control group (0.537; 0.497; 0.282 and 0.239 in control, DU10, DU20 and DU40 groups respectively) whereas no accumulation of DU was recorded in the ovaries whatever the dose tested. Abnormal perivitelline space (P < 0.001) or absence of the 1st polar body (P < 0.001) was identified as the main characteristic of DU impact. In the context of this study, the NOAEL for oocyte quality was determined at 10 mg L?1 in drinking water (1.9 mg kg?1 day?1). An increase in the dose of contamination over 20 mg L?1 did not amplify the proportion of oocytes contracting a specific alteration but conducted to a diversification in oocytes abnormalities. Further investigations are necessary to correlate morphologic assessment of female gamete with its developmental competence.

Alexandre Feugier; Sandrine Frelon; Patrick Gourmelon; Marie Claraz

2008-01-01T23:59:59.000Z

150

Stable yeast transformants that secrete functional. cap alpha. -amylase encoded by cloned mouse pancreatic cDNA  

SciTech Connect (OSTI)

Mouse pancreatic ..cap alpha..-amylase complementary DNA was inserted into a yeast shuttle vector after the Saccharomyces cerevisiae MF..cap alpha..1 promoter and secretion signals coding sequences. When transformed with the recombinant plasmid, S. cerevisiae cells were able to synthesize and secrete functional ..cap alpha..-amylase, efficiently hydrolyzing starch present in the culture medium. Stable amylolytic cells were obtained from different yeast strains. This work represents a significant step towards producing yeast that can convert starchy materials directly to ethanol.

Filho, S.A.; Galembeck, E.V.; Faria, J.B.; Frascino, A.C.S.

1986-04-01T23:59:59.000Z

151

Hyperspectral imaging signatures detect amyloidopathy in Alzheimer’s mouse retina well before onset of cognitive decline  

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The technique was found useful for monitoring retinal and brain amyloidopathy in an ongoing preclinical anti-AD study, attesting to the technique’s sensitivity and specificity. ... Interestingly, the technique was found applicable not just to excised brain tissue, but also to isolated mouse retina. ... With the retina being heralded widely as a (diagnostic) extension of the CNS and retinal amyloidopathy occurring well before that in the brain, this development raises a possibility for the first direct retinal imaging diagnosis of early, asymptomatic Alzheimer’s disease. ...

Swati Sudhakar More; Robert Vince

2014-10-29T23:59:59.000Z

152

GEORGE, J. C. 1962. A histophysiological study ofthe red and white mus-  

E-Print Network [OSTI]

),The physiological ecology of tunas. Academic Press, N.Y. HALL. F. G. 1930. The ability of the common mackerel, R. C. L. 1973. On the function of the white muscles in teleosts at intermediate swimming speeds. J.). Compo Biochem. Physiol. 49B:367-373. JOHNSTON, I. A., W. DAVISON, AND G. GOLDSPINK. 1977. Energy

153

Identification of the Myelin Protein Plasmolipin as the Cell Entry Receptor for Mus caroli Endogenous Retrovirus  

Science Journals Connector (OSTI)

...Genome Center website ( http://www-shgc.stanford.edu/seq/rhserverformnew...localized to chromosome 16q13 closest to marker SHGC-34581, with a highly significant logarithm...top-scoring markers were all located close to SHGC-34581 on chromosome 16, with a decreasing...

A. Dusty Miller; Ulla Bergholz; Marion Ziegler; Carol Stocking

2008-05-07T23:59:59.000Z

154

Genotoksisitet av 5-hydroxymetylfurfural i transgene LacZ mus med mutasjon i adenomatøs polyposis coli.  

E-Print Network [OSTI]

??5-hydroxymethylfurfural (HMF) is formed in the Maillard reaction when reduced sugars are heated in the presence of amino acids or proteins. It can also be… (more)

Jørgensen-Kaur, Lena

2012-01-01T23:59:59.000Z

155

PriMus by Guido Cianciulli -copyright ACCA software S.p.A. COMMITTENTE  

E-Print Network [OSTI]

-jersey, dotate di S01.01.001.1 tappi di introduzione ed evacuazione, da riempire con acqua o sabbia per un peso mediante barriere in polietilene tipo new-jersey, dotate di S01.01.001.1 tappi di introduzione ed

Guidoni, Leonardo

156

Transient inhibition of cell proliferation does not compromise self-renewal of mouse embryonic stem cells  

SciTech Connect (OSTI)

Embryonic stem cells (ESCs) have unlimited capacity for self-renewal and can differentiate into various cell types when induced. They also have an unusual cell cycle control mechanism driven by constitutively active cyclin dependent kinases (Cdks). In mouse ESCs (mESCs). It is proposed that the rapid cell proliferation could be a necessary part of mechanisms that maintain mESC self-renewal and pluripotency, but this hypothesis is not in line with the finding in human ESCs (hESCs) that the length of the cell cycle is similar to differentiated cells. Therefore, whether rapid cell proliferation is essential for the maintenance of mESC state remains unclear. We provide insight into this uncertainty through chemical intervention of mESC cell cycle. We report here that inhibition of Cdks with olomoucine II can dramatically slow down cell proliferation of mESCs with concurrent down-regulation of cyclin A, B and E, and the activation of the Rb pathway. However, mESCs display can recover upon the removal of olomoucine II and are able to resume normal cell proliferation without losing self-renewal and pluripotency, as demonstrated by the expression of ESC markers, colony formation, embryoid body formation, and induced differentiation. We provide a mechanistic explanation for these observations by demonstrating that Oct4 and Nanog, two major transcription factors that play critical roles in the maintenance of ESC properties, are up-regulated via de novo protein synthesis when the cells are exposed to olomoucine II. Together, our data suggest that short-term inhibition of cell proliferation does not compromise the basic properties of mESCs. -- Highlights: Black-Right-Pointing-Pointer Inhibition of Cdks slows down mESCs proliferation. Black-Right-Pointing-Pointer mESCs display remarkable recovery capacity from short-term cell cycle interruption. Black-Right-Pointing-Pointer Short-term cell cycle interruption does not compromise mESC self-renewal. Black-Right-Pointing-Pointer Oct4 and Nanog are up-regulated via de novo synthesis by cell cycle interruption.

Wang, Ruoxing [Department of Biological Sciences, The University of Southern Mississippi, 118 College Drive 5018, Hattiesburg, MS 39406 (United States)] [Department of Biological Sciences, The University of Southern Mississippi, 118 College Drive 5018, Hattiesburg, MS 39406 (United States); Guo, Yan-Lin, E-mail: yanlin.guo@usm.edu [Department of Biological Sciences, The University of Southern Mississippi, 118 College Drive 5018, Hattiesburg, MS 39406 (United States)] [Department of Biological Sciences, The University of Southern Mississippi, 118 College Drive 5018, Hattiesburg, MS 39406 (United States)

2012-10-01T23:59:59.000Z

157

Slow elimination of phosphorylated histone {gamma}-H2AX from DNA of terminally differentiated mouse heart cells in situ  

SciTech Connect (OSTI)

Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around double-strand DNA breaks (DSBs) and this modification (called {gamma}-H2AX) may serve as a useful marker of genome damage and repair in terminally differentiated cells. Here using immunohistochemistry we studied kinetics of {gamma}-H2AX formation and elimination in the X-irradiated mouse heart and renal epithelial tissues in situ. Unirradiated tissues have 3-5% {gamma}-H2AX-positive cells and in tissues fixed 1 h after X-irradiation {gamma}-H2AX-positive nuclei are induced in a dose-dependent manner approaching 20-30% after 3 Gy of IR. Analysis of mouse tissues at different times after 3 Gy of IR showed that maximal induction of {gamma}-H2AX in heart is observed 20 min after IR and then is decreased slowly with about half remaining 23 h later. In renal epithelium maximum of the {gamma}-H2AX-positive cells is observed 40 min after IR and then decreases to control values in 23 h. This indicates that there are significant variations between non-proliferating mammalian tissues in the initial H2AX phosphorylation rate as well as in the rate of {gamma}-H2AX elimination after X-irradiation, which should be taken into account in the analysis of radiation responses.

Gavrilov, Boris [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Vezhenkova, Irina [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Firsanov, Denis [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Solovjeva, Liudmila [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Svetlova, Maria [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Mikhailov, Vyacheslav [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation); Tomilin, Nikolai [Institute of Cytology, Russian Academy of Sciences, 194064 St. Petersburg (Russian Federation)]. E-mail: nvtom@hotmail.com

2006-09-08T23:59:59.000Z

158

Ii.p'r":~.~~  

E-Print Network [OSTI]

HISTORY LITERATURE - Select 9 hours MUS 3114 Symphonic Literature 3 MUS 3124 20th Century Literature 3 MUS MUS 3055 Music and Media Production I 3 MUS 3056 Music and Media Production II 3 MUS 3065 Camp. Mus. and Multimedia I 3 MUS 3066 Camp. Mus. and Multimedia II 3 (1) __ (1) __ (1) __ (1) __ (1) __ (1)-_ VIII

Virginia Tech

159

Susceptibility of Highly Pathogenic H5N1 Influenza Viruses to the Neuraminidase Inhibitor Oseltamivir Differs In Vitro and in a Mouse Model  

Science Journals Connector (OSTI)

...the proportional hazards model. e Loss or gain of weight...R. 1972. Regression models and life-tables. J...Kawaoka. 2005. Avian flu: isolation of drug-resistant...in vitro and in a mouse model. | While the neuraminidase...whether in vitro testing can predict the in vivo effectiveness...

Elena A. Govorkova; Natalia A. Ilyushina; Jennifer L. McClaren; Tri S. P. Naipospos; Bounlom Douangngeun; Robert G. Webster

2009-04-06T23:59:59.000Z

160

Rapid, automated imaging of mouse articular cartilage by microCT for early detection of osteoarthritis and finite element modelling of joint mechanics  

Science Journals Connector (OSTI)

SummaryObjective Mouse articular cartilage (AC) is mostly assessed by histopathology and its mechanics is poorly characterised. In this study: (1) we developed non-destructive imaging for quantitative assessment of AC morphology and (2) evaluated the mechanical implications of AC structural changes. Methods Knee joints obtained from naïve mice and from mice with osteoarthritis (OA) induced by destabilization of medial meniscus (DMM) for 4 and 12 weeks, were imaged by phosphotungstic acid (PTA) contrast enhanced micro-computed tomography (PTA-CT) and scored by conventional histopathology. Our software (Matlab) automatically segmented tibial AC, drew two regions centred on each tibial condyle and evaluated the volumes included. A finite element (FE) model of the whole mouse joint was implemented to evaluate AC mechanics. Results Our method achieved rapid, automated analysis of mouse AC (structural parameters in simulations estimated that AC thinning at early-stages in the DMM model (4 weeks) increases contact pressures (+39%) and Tresca stresses (+43%) in AC. Conclusion PTA-CT imaging is a fast and simple method to assess OA in murine models. Once applied more extensively to confirm its robustness, our approach will be useful for rapidly phenotyping genetically modified mice used for OA research and to improve the current understanding of mouse cartilage mechanics.

P. Das Neves Borges; A.E. Forte; T.L. Vincent; D. Dini; M. Marenzana

2014-01-01T23:59:59.000Z

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161

Mandibular and Neural Crest Cell Deficits Seen in TsDn65 Down Syndrome Mouse Model Rescued By Green Tea Polyphenol, EGCG  

E-Print Network [OSTI]

to the mandibular deficit seen in E9.5 Ts65Dn embryos. We propose that EGCG, a green tea polyphenol, will inhibitMandibular and Neural Crest Cell Deficits Seen in TsDn65 Down Syndrome Mouse Model Rescued By Green Tea Polyphenol, EGCG Gracelyn C. Bose1 , Rachel A. Novack1 ,Danika M. Tumbleson1 , Alexis N. Chom1

Zhou, Yaoqi

162

Local volume changes of the corpus callosum from 3D MR images of wildtype and knockout mouse brains G. Hamarneh1  

E-Print Network [OSTI]

CC from the MR images (Figure 1a) using Livewire, a semi-automatic segmentation tool provided by AmiraLocal volume changes of the corpus callosum from 3D MR images of wildtype and knockout mouse brains G. Hamarneh1 , J. Chen1 , N. Lifshitz1 , J. Henderson2 , M. Henkelman1 1 Hospital for Sick Children

Hamarneh, Ghassan

163

The Teratogenic Sensitivity to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Is Modified by a Locus on Mouse Chromosome 3  

E-Print Network [OSTI]

- phrosis in developing mice exposed to the pollutant 2,3,7,8- tetrachlorodibenzo-p-dioxin (dioxin) is a prototype for the study of gene-environment interactions. Dioxin is an ubiquitous environmental pollutantThe Teratogenic Sensitivity to 2,3,7,8-Tetrachlorodibenzo-p- dioxin Is Modified by a Locus on Mouse

Bradfield, Christopher A.

164

Direct and indirect impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)1 on adult mouse Leydig cells: an in vitro study.2  

E-Print Network [OSTI]

1 Direct and indirect impact of 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD)1 on adult mouse Leydig,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related substances are ubiquitous22 environmental pollutants that exert words: Dioxin; Leydig cell; in vitro; AhR; chemokine39 40 inserm-00816472,version1-22Apr2013 #12;3 1

Paris-Sud XI, Université de

165

Conversion of the Common Food Constituent 5-Hydroxymethylfurfural into a Mutagenic and Carcinogenic Sulfuric Acid Ester in the Mouse in Vivo  

Science Journals Connector (OSTI)

Conversion of the Common Food Constituent 5-Hydroxymethylfurfural into a Mutagenic and Carcinogenic Sulfuric Acid Ester in the Mouse in Vivo ... 5-Hydroxymethylfurfural (HMF), formed by acid-catalyzed dehydration and in the Maillard reaction from reducing sugars, is found at high levels in numerous foods. ... Hydroxymethylfurfural, A Versatile Platform Chemical Made from Renewable Resources ...

Bernhard H. Monien; Heinz Frank; Albrecht Seidel; Hansruedi Glatt

2009-04-22T23:59:59.000Z

166

Simultaneous submicrometric 3D imaging of the micro-vascular network and the neuronal system in a mouse spinal cord  

E-Print Network [OSTI]

Defaults in vascular (VN) and neuronal networks of spinal cord are responsible for serious neurodegenerative pathologies. Because of inadequate investigation tools, the lacking knowledge of the complete fine structure of VN and neuronal systems is a crucial problem. Conventional 2D imaging yields incomplete spatial coverage leading to possible data misinterpretation, whereas standard 3D computed tomography imaging achieves insufficient resolution and contrast. We show that X-ray high-resolution phase-contrast tomography allows the simultaneous visualization of three-dimensional VN and neuronal systems of mouse spinal cord at scales spanning from millimeters to hundreds of nanometers, with neither contrast agent nor a destructive sample-preparation. We image both the 3D distribution of micro-capillary network and the micrometric nerve fibers, axon-bundles and neuron soma. Our approach is a crucial tool for pre-clinical investigation of neurodegenerative pathologies and spinal-cord-injuries. In particular, it s...

Fratini, Michela; Campi, Gaetano; Brun, Francesco; Tromba, Giuliana; Modregger, Peter; Bucci, Domenico; Battaglia, Giuseppe; Spadon, Raffaele; Mastrogiacomo, Maddalena; Requardt, Herwig; Giove, Federico; Bravin, Alberto; Cedola, Alessia

2014-01-01T23:59:59.000Z

167

Larder of the Red-Backed Shrike  

Science Journals Connector (OSTI)

... , and occasionally barbed wire is also used. Larders often have a considerable number of humble-bees (Bombus) in them, often a small bird and, occasionally, a house- ... a small bird and, occasionally, a house-mouse (Mus domesticus) is found. Queen humble-bees figure prominently in the larders. The birds usually devour workers and males, carder ...

T. H. HAWKINS

1948-09-25T23:59:59.000Z

168

Cyclic mechanical strain maintains Nanog expression through PI3K/Akt signaling in mouse embryonic stem cells  

SciTech Connect (OSTI)

Mechanical strain has been reported to affect the proliferation/differentiation of many cell types; however, the effects of mechanotransduction on self-renewal as well as pluripotency of embryonic stem (ES) cells remains unknown. To investigate the effects of mechanical strain on mouse ES cell fate, we examined the expression of Nanog, which is an essential regulator of self-renewal and pluripotency as well as Nanog-associated intracellular signaling during uniaxial cyclic mechanical strain. The mouse ES cell line, CCE was plated onto elastic membranes, and we applied 10% strain at 0.17 Hz. The expression of Nanog was reduced during ES cell differentiation in response to the withdrawal of leukemia inhibitory factor (LIF); however, two days of cyclic mechanical strain attenuated this reduction of Nanog expression. On the other hand, the cyclic mechanical strain promoted PI3K-Akt signaling, which is reported as an upstream of Nanog transcription. The cyclic mechanical strain-induced Akt phosphorylation was blunted by the PI3K inhibitor wortmannin. Furthermore, cytochalasin D, an inhibitor of actin polymerization, also inhibited the mechanical strain-induced increase in phospho-Akt. These findings imply that mechanical force plays a role in regulating Nanog expression in ES cells through the actin cytoskeleton-PI3K-Akt signaling. -- Highlights: Black-Right-Pointing-Pointer The expression of Nanog, which is an essential regulator of 'stemness' was reduced during embryonic stem (ES) cell differentiation. Black-Right-Pointing-Pointer Cyclic mechanical strain attenuated the reduction of Nanog expression. Black-Right-Pointing-Pointer Cyclic mechanical strain promoted PI3K-Akt signaling and mechanical strain-induced Akt phosphorylation was blunted by the PI3K inhibitor and an inhibitor of actin polymerization.

Horiuchi, Rie [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan)] [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan); Akimoto, Takayuki, E-mail: akimoto@m.u-tokyo.ac.jp [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan) [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan); Institute for Biomedical Engineering, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Waseda-tsurumaki, Shinjuku, Tokyo 162-0041 (Japan); Hong, Zhang [Institute for Biomedical Engineering, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Waseda-tsurumaki, Shinjuku, Tokyo 162-0041 (Japan)] [Institute for Biomedical Engineering, Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Waseda-tsurumaki, Shinjuku, Tokyo 162-0041 (Japan); Ushida, Takashi [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan)] [Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033 (Japan)

2012-08-15T23:59:59.000Z

169

A Novel mouse model of enhanced proteostasis: Full-length human heat shock factor 1 transgenic mice  

SciTech Connect (OSTI)

Research highlights: {yields} Development of mouse overexpressing native human HSF1 in all tissues including CNS. {yields} HSF1 overexpression enhances heat shock response at whole-animal and cellular level. {yields} HSF1 overexpression protects from polyglutamine toxicity and favors aggresomes. {yields} HSF1 overexpression enhances proteostasis at the whole-animal and cellular level. -- Abstract: The heat shock response (HSR) is controlled by the master transcriptional regulator heat shock factor 1 (HSF1). HSF1 maintains proteostasis and resistance to stress through production of heat shock proteins (HSPs). No transgenic model exists that overexpresses HSF1 in tissues of the central nervous system (CNS). We generated a transgenic mouse overexpressing full-length non-mutant HSF1 and observed a 2-4-fold increase in HSF1 mRNA and protein expression in all tissues studied of HSF1 transgenic (HSF1{sup +/0}) mice compared to wild type (WT) littermates, including several regions of the CNS. Basal expression of HSP70 and 90 showed only mild tissue-specific changes; however, in response to forced exercise, the skeletal muscle HSR was more elevated in HSF1{sup +/0} mice compared to WT littermates and in fibroblasts following heat shock, as indicated by levels of inducible HSP70 mRNA and protein. HSF1{sup +/0} cells elicited a significantly more robust HSR in response to expression of the 82 repeat polyglutamine-YFP fusion construct (Q82YFP) and maintained proteasome-dependent processing of Q82YFP compared to WT fibroblasts. Overexpression of HSF1 was associated with fewer, but larger Q82YFP aggregates resembling aggresomes in HSF1{sup +/0} cells, and increased viability. Therefore, our data demonstrate that tissues and cells from mice overexpressing full-length non-mutant HSF1 exhibit enhanced proteostasis.

Pierce, Anson, E-mail: piercea2@uthscsa.edu [Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States) [Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); The Department of Veteran's Affairs, South Texas Veterans Health Care System, San Antonio, Texas, 78284 (United States); Wei, Rochelle; Halade, Dipti [Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States)] [Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); Yoo, Si-Eun [Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States) [Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); Ran, Qitao; Richardson, Arlan [Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States) [Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); Barshop Institute for Longevity and Aging Studies, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78229 (United States); The Department of Veteran's Affairs, South Texas Veterans Health Care System, San Antonio, Texas, 78284 (United States)

2010-11-05T23:59:59.000Z

170

Functional Analysis of the Mouse Scn8a Sodium Channel Marianne R. Smith,1 Raymond D. Smith,1 Nicholas W. Plummer,2 Miriam H. Meisler,2 and Alan L. Goldin1  

E-Print Network [OSTI]

Functional Analysis of the Mouse Scn8a Sodium Channel Marianne R. Smith,1 Raymond D. Smith,1 modulate the electrophysiological properties of the channels (Isom et al., 1992, 1995; Smith and Goldin

Goldin, Alan L.

171

Genetic susceptibility to low-dose ionizing radiation in the mouse mammary glandas a means of understanding human risk for breast cancer  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

susceptibility to low-dose ionizing radiation in the mouse mammary gland susceptibility to low-dose ionizing radiation in the mouse mammary gland as a means of understanding human risk for breast cancer Antoine M. Snijders 1 , Francesco Marchetti 1 , Ju Han 1 , Sandhya Bhatnagar 1 , Nadire Duru 1 , Zhi Hu 1 , Jian-Hua Mao 1 , Mina Bissell 1 , Joe Gray 1,2 , Gary H. Karpen 1 , Priscilla K. Cooper 1 and Andrew J. Wyrobek 1 1 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 2 Current affiliation: Biomedical Engineering, Oregon Health Science Univ, Portland, OR Goal: Our goal is to develop an in vivo mechanistic model of genetic variation in the low-dose damage responses of mammary glands using inbred mice known to vary in their sensitivity to low-dose induced mammary gland cancer, and to develop molecular predictors for susceptibility or resistance to low-dose induced breast cancer.

172

The role of renal proximal tubule P450 enzymes in chloroform-induced nephrotoxicity: Utility of renal specific P450 reductase knockout mouse models  

SciTech Connect (OSTI)

The kidney is a primary target for numerous toxic compounds. Cytochrome P450 enzymes (P450) are responsible for the metabolic activation of various chemical compounds, and in the kidney are predominantly expressed in proximal tubules. The aim of this study was to test the hypothesis that renal proximal tubular P450s are critical for nephrotoxicity caused by chemicals such as chloroform. We developed two new mouse models, one having proximal tubule-specific deletion of the cytochrome P450 reductase (Cpr) gene (the enzyme required for all microsomal P450 activities), designated proximal tubule-Cpr-null (PTCN), and the other having proximal tubule-specific rescue of CPR activity with the global suppression of CPR activity in all extra-proximal tubular tissues, designated extra-proximal tubule-Cpr-low (XPT-CL). The PTCN, XPT-CL, Cpr-low (CL), and wild-type (WT) mice were treated with a single oral dose of chloroform at 200 mg/kg. Blood, liver and kidney samples were obtained at 24 h after the treatment. Renal toxicity was assessed by measuring BUN and creatinine levels, and by pathological examination. The blood and tissue levels of chloroform were determined. The severity of toxicity was less in PTCN and CL mice, compared with that of WT and XPT-CL mice. There were no significant differences in chloroform levels in the blood, liver, or kidney, between PTCN and WT mice, or between XPT-CL and CL mice. These findings indicate that local P450-dependent activities play an important role in the nephrotoxicity induced by chloroform. Our results also demonstrate the usefulness of these novel mouse models for studies of chemical-induced kidney toxicity. - Highlights: • New mouse models were developed with varying P450 activities in the proximal tubule. • These mouse models were treated with chloroform, a nephrotoxicant. • Studies showed the importance of local P450s in chloroform-induced nephrotoxicity.

Liu, Senyan [Kidney Institute and Division of Nephrology, Changzheng Hospital, Shanghai 200003 (China); Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York, Albany, NY 12201 (United States); Yao, Yunyi; Lu, Shijun; Aldous, Kenneth; Ding, Xinxin [Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York, Albany, NY 12201 (United States); Mei, Changlin, E-mail: chlmei1954@126.com [Kidney Institute and Division of Nephrology, Changzheng Hospital, Shanghai 200003 (China); Gu, Jun, E-mail: jungu@wadsworth.org [Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York, Albany, NY 12201 (United States)

2013-10-01T23:59:59.000Z

173

Impaired Organization and Function of Myofilaments in Single Muscle Fibers from a Mouse Model of Pompe Disease  

SciTech Connect (OSTI)

Pompe disease, a deficiency of lysosomal acid {alpha}-glucosidase, is a disorder of glycogen metabolism that can affect infants, children, or adults. In all forms of the disease, there is progressive muscle pathology leading to premature death. The pathology is characterized by accumulation of glycogen in lysosomes, autophagic buildup, and muscle atrophy. The purpose of the present investigation was to determine if myofibrillar dysfunction in Pompe disease contributes to muscle weakness beyond that attributed to atrophy. The study was performed on isolated myofibers dissected from severely affected fast glycolytic muscle in the {alpha}-glucosidase knockout mouse model. Psoas muscle fibers were first permeabilized, so that the contractile proteins could be directly relaxed or activated by control of the composition of the bathing solution. When normalized by cross-sectional area, single fibers from knockout mice produced 6.3 N/cm{sup 2} of maximum Ca{sup 2+}-activated tension compared with 12.0 N/cm{sup 2} produced by wild-type fibers. The total protein concentration was slightly higher in the knockout mice, but concentrations of the contractile proteins myosin and actin remained unchanged. Structurally, X-ray diffraction showed that the actin and myosin filaments, normally arranged in hexagonal arrays, were disordered in the knockout muscle, and a lower fraction of myosin cross bridges was near the actin filaments in the relaxed muscle. The results are consistent with a disruption of actin and myosin interactions in the knockout muscles, demonstrating that impaired myofibrillar function contributes to weakness in the diseased muscle fibers.

Xu, S.; Galperin, M; Melvin, G; Horowits, R; Raben, N; Plotz, P; Yu, L

2010-01-01T23:59:59.000Z

174

Ann. Naturhist. Mus. Wien, Serie A 373509 Wien, Mai 2011113 Gastropods and their habitats from the northern Red Sea  

E-Print Network [OSTI]

and on some rocky tidal flats. Rissoidae and Plesiotrochidae were mostly found in coarse-grained sediments flats near the reef edge. Strombidae and Turritellidae are widely distributed on soft- and hard 1

Zuschin, Martin

175

MUS420/EE367A Supplement to Lecture 7B: Wave Digital Filters and Waveguide Networks for  

E-Print Network [OSTI]

-time energy. Classical Kirchoff Network · Basic component: N-port i1 i2 iN v1 v2 vN . . . · Characterized by port voltages vk and currents ik, k = 1 . . . N. 2 #12;· N-ports to be connected portwise · Instantaneous power absorbed by the N-port through the ports is pinst = N j=1 vjij Passivity A given N-port

Smith III, Julius Orion

176

MUS420/EE367A Supplement to Lecture 7B: Wave Digital Filters and Waveguide Networks for  

E-Print Network [OSTI]

-time energy. Classical Kirchoff Network · Basic component: N-port i1 i2 iN v1 v2 vN . . . · Characterized by port voltages vk and currents ik, k = 1 . . . N. 2 · N-ports to be connected portwise · Instantaneous power absorbed by the N-port through the ports is pinst = N j=1 vjij Passivity A given N-port satisfies

Smith III, Julius Orion

177

Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.  

Science Journals Connector (OSTI)

...status of each gene; (ii) thermal stability of genomic DNA-cDNA...we digested mouse DNA with a battery of enzymes and subjected the...pseudogene, respectively. Thermal stability of S16 cDNA-S16...Based on this reasoning, a thermal stability experiment was performed...

M Wagner; R P Perry

1985-12-01T23:59:59.000Z

178

Exploring the in vitro formation of trimethylarsine sulfide from dimethylthioarsinic acid in anaerobic microflora of mouse cecum using HPLC-ICP-MS and HPLC-ESI-MS  

SciTech Connect (OSTI)

Although metabolism of arsenicals to form methylated oxoarsenical species has been extensively studied, less is known about the formation of thiolated arsenical species that have recently been detected as urinary metabolites. Indeed, their presence suggests that the metabolism of ingested arsenic is more complex than previously thought. Recent reports have shown that thiolated arsenicals can be produced by the anaerobic microflora of the mouse cecum, suggesting that metabolism prior to systemic absorption may be a significant determinant of the pattern and extent of exposure to various arsenic-containing species. Here, we examined the metabolism of {sup 34}S labeled dimethylthioarsinic acid ({sup 34}S-DMTA{sup V}) by the anaerobic microflora of the mouse cecum using HPLC-ICP-MS and HPLC-ESI-MS/MS to monitor for the presence of various oxo- and thioarsenicals. The use of isotopically enriched {sup 34}S-DMTA{sup V} made it possible to differentiate among potential metabolic pathways for production of the trimethylarsine sulfide (TMAS{sup V}). Upon in vitro incubation in an assay containing anaerobic microflora of mouse cecum, {sup 34}S-DMTA{sup V} underwent several transformations. Labile {sup 34}S was exchanged with more abundant {sup 32}S to produce {sup 32}S-DMTA{sup V}, a thiol group was added to yield DMDTA{sup V}, and a methyl group was added to yield {sup 34}S-TMAS{sup V}. Because incubation of {sup 34}S-DMTA{sup V} resulted in the formation of {sup 34}S-TMAS{sup V}, the pathway for its formation must preserve the arsenic-sulfur bond. The alternative metabolic pathway postulated for formation of TMAS{sup V} from dimethylarsinic acid (DMA{sup V}) would proceed via a dimethylarsinous acid (DMA{sup III}) intermediate and would necessitate the loss of {sup 34}S label. Structural confirmation of the metabolic product was achieved using HPLC-ESI-MS/MS. The data presented support the direct methylation of DMTA{sup V} to TMAS{sup V}. Additionally, the detection of isotopically pure {sup 34}S-TMAS{sup V} raises questions about the sulfur exchange properties of TMAS{sup V} in the cecum material. Therefore, {sup 34}S-TMAS{sup V} was incubated and the exchange was monitored with respect to time. The data suggest that the As-S bond associated with TMAS{sup V} is less labile than the As-S bond associated with DMTA{sup V}.

Kubachka, Kevin M. [US EPA, ORD, NERL, Microbiological and Chemical Exposure, Assessment Research Division, Cincinnati, OH 45268 (United States); Kohan, Michael C. [US EPA, ORD, NHEERL, Environmental Carcinogenesis Division, Research Triangle Park, NC 27711 (United States); Herbin-Davis, Karen [US EPA, ORD, NHEERL, Experimental Toxicology Division, Research Triangle Park, NC 27711 (United States); Creed, John T. [US EPA, ORD, NERL, Microbiological and Chemical Exposure, Assessment Research Division, Cincinnati, OH 45268 (United States)], E-mail: creed.jack@epa.gov; Thomas, David J. [US EPA, ORD, NHEERL, Experimental Toxicology Division, Research Triangle Park, NC 27711 (United States)

2009-09-01T23:59:59.000Z

179

Parg deficiency confers radio-sensitization through enhanced cell death in mouse ES cells exposed to various forms of ionizing radiation  

SciTech Connect (OSTI)

Highlights: •Parg{sup ?/?} ES cells were more sensitive to ?-irradiation than Parp-1{sup ?/?} ES cells. •Parg{sup ?/?} cells were more sensitive to carbon-ion irradiation than Parp-1{sup ?/?} cells. •Parg{sup ?/?} cells showed defects in DSB repair after carbon-ion irradiation. •PAR accumulation was enhanced after carbon-ion irradiation compared to ?-irradiation. -- Abstract: Poly(ADP-ribose) glycohydrolase (Parg) is the main enzyme involved in poly(ADP-ribose) degradation. Here, the effects of Parg deficiency on sensitivity to low and high linear-energy-transfer (LET) radiation were investigated in mouse embryonic stem (ES) cells. Mouse Parg{sup ?/?} and poly(ADP-ribose) polymerase-1 deficient (Parp-1{sup ?/?}) ES cells were used and responses to low and high LET radiation were assessed by clonogenic survival and biochemical and biological analysis methods. Parg{sup ?/?} cells were more sensitive to ?-irradiation than Parp-1{sup ?/?} cells. Transient accumulation of poly(ADP-ribose) was enhanced in Parg{sup ?/?} cells. Augmented levels of phosphorylated H2AX (?-H2AX) from early phase were observed in Parg{sup ?/?} ES cells. The induction level of p53 phophorylation at ser18 was similar in wild-type and Parp-1{sup ?/?} cells and apoptotic cell death process was mainly observed in the both genotypes. These results suggested that the enhanced sensitivity of Parg{sup ?/?} ES cells to ?-irradiation involved defective repair of DNA double strand breaks. The effects of Parg and Parp-1 deficiency on the ES cell response to carbon-ion irradiation (LET13 and 70 keV/?m) and Fe-ion irradiation (200 keV/?m) were also examined. Parg{sup ?/?} cells were more sensitive to LET 70 keV/?m carbon-ion irradiation than Parp-1{sup ?/?} cells. Enhanced apoptotic cell death also accompanied augmented levels of ?-H2AX in a biphasic manner peaked at 1 and 24 h. The induction level of p53 phophorylation at ser18 was not different between wild-type and Parg{sup ?/?} cells. The augmented level of poly(ADP-ribose) accumulation was noted after carbon-ion irradiation compared to ?-irradiation even in the wild-type cells. An enhanced poly(ADP-ribose) accumulation was further observed in Parg{sup ?/?} cells. Both Parg{sup ?/?} cells and Parp-1{sup ?/?} cells did not show sensitization to Fe-ion irradiation. Parg deficiency sensitizes mouse ES cells to a wide therapeutic range of LET radiation through the effects on DNA double strand break repair responses and enhanced cell death.

Shirai, Hidenori; Fujimori, Hiroaki [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Gunji, Akemi [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Maeda, Daisuke [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Hirai, Takahisa [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan); Poetsch, Anna R. [ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Harada, Hiromi [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)] [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Yoshida, Tomoko [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minatoku, Tokyo 105-8512 (Japan); Sasai, Keisuke [Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan)] [Department of Radiation Oncology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 (Japan); Okayasu, Ryuichi [International Open Laboratory, National Institute of Radiological Science, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan)] [International Open Laboratory, National Institute of Radiological Science, 4-9-1 Anagawa, Inage, Chiba 263-8555 (Japan); Masutani, Mitsuko, E-mail: mmasutan@ncc.go.jp [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan) [Division of Genome Stability Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); Biochemistry Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan); ADP-Ribosylation in Oncology Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan)

2013-05-24T23:59:59.000Z

180

Attenuation of Mouse Melanoma by A/C Magnetic Field after Delivery of Bi-Magnetic Nanoparticles by Neural Progenitor Cells  

Science Journals Connector (OSTI)

Attenuation of Mouse Melanoma by A/C Magnetic Field after Delivery of Bi-Magnetic Nanoparticles by Neural Progenitor Cells ... 0.5% w/w) and clinically acceptable magnetic fields, intratumoral power absorption is comparable to RF heating with local applicators and superior to regional RF heating (by comparison with clinical SAR measurements from regional and local hyperthermia treatments). ... With the exception of the K562 cell line, all 9 leukemic cell lines tested were resistant to the natural killer activity of freshly isolated peripheral blood lymphocytes (PBL) from healthy donors but were susceptible to the lytic action of PBL cultured for 3 days in the presence of rIL-2. ...

Raja Shekar Rachakatla; Sivasai Balivada; Gwi-Moon Seo; Carl B. Myers; Hongwang Wang; Thilani N. Samarakoon; Raj Dani; Marla Pyle; Franklin O. Kroh; Brandon Walker; Xiaoxuan Leaym; Olga B. Koper; Viktor Chikan; Stefan H. Bossmann; Masaaki Tamura; Deryl L. Troyer

2010-11-08T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
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they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

Fatal lymphoreticular disease in the scurfy (sf) mouse requires T cells that mature in a sf thymic environment: Potential model for thymic education  

SciTech Connect (OSTI)

Characteristic lesions in mice hemi- or homozygous for the X-linked mutation scurfy (sf) include lymphohistiocytic proliferation in the skin and lymphoid organs, Coombs' test-positive anemia, hypergammaglobulinemia, and death by 24 days of age. The role of the thymus in the development of fatal lymphoreticular disease in the scurfy mouse was investigated. Neonatal thymectomy doubles the life span of scurfy mice, moderates the histologic lesions, and prevents anemia, despite the continued presence of high levels of serum IgG. Animals bred to be nude and scurfy (nu/nu;sf/Y) are viable, fertile, and free of scurfy lesions. Bone marrow from scurfy mice can reconstitute lethally irradiated, H-2-compatible animals but does not transmit scurfy disease. The authors conclude, from these data, that scurfy lesions are mediated by T lymphocytes that mature in an abnormal (sf) thymic environment.

Godfrey, V.L.; Rinchik, E.M.; Russell, L.B. (Oak Ridge National Lab., Oak Ridge, TN (United States)); Wilkinson, J.E. (Univ. of Tennessee, Knoxville (United States))

1991-07-01T23:59:59.000Z

182

Using ex vivo organ culture models as surrogates to investigate morphological and functional differences of mammary glands derived from mouse strains that differ in cancer susceptibility to understand the underlying mechanisms of radiation sensitivity or resistance  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

ex vivo organ culture models as surrogates to investigate morphological and functional ex vivo organ culture models as surrogates to investigate morphological and functional differences of mammary glands derived from mouse strains that differ in cancer susceptibility to understand the underlying mechanisms of radiation sensitivity or resistance Alvin Lo, Joni D. Mott, Jian-Hua Mao, and Mina J. Bissell Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley CA 94720 Goal: Within the Lawrence Berkeley National Laboratory's Low Dose SFA, as part of Project 2, we are using a systems genetics approach to determine the contribution of non-targeted and targeted radiation effects for risk of mammary carcinogenesis. The goal of this work is to characterize the mammary gland of the parental mouse strains, and the F1 and F2 generations used in these studies with respect to tissue

183

Photoaffinity Labeling of Mouse Fibroblast Enzymes by a Base Excision Repair Intermediate: New Evidence on the Role of PARP-1 in DNA Repair  

SciTech Connect (OSTI)

To examine mammalian base excision repair (BER) enzymes interacting with DNA intermediates formed during BER, we used a novel photoaffinity labeling probe and mouse embryonic fibroblast (MEF) crude extract. The probe was formed in situ, using an end-labeled oligonucleotide containing a synthetic abasic site; this site was incised by AP endonuclease creating a nick with 3' hydroxyl and 5' reduced sugar phosphate groups at the margins, and then a dNMP carrying a photoreactive adduct was introduced at the 3' hydroxyl group. With near UV-light exposure (312nm) of the extract-probe mixture, only six proteins were strongly labeled, including poly (ADP-ribose) polymerase (PARP-1) and the well-known BER participants flap endonuclease (FEN-1), DNA polymerase b (b-pol), and AP endonuclease (APE). The amount of probe crosslinked to PARP-1 was greater than that crosslinked to the other proteins. The specificity of PARP-1 labeling was examined by competition experiments involving various oligonucleotide competitors; competition of labeling by the probe was much greater for the BER intermediates tested than for normal double-stranded DNA. The specificity of PARP-1 labeling also was examined using DNA probes with alternate structures; PARP-1 labeling was stronger with a DNA oligomer representing a BER intermediate than with a molecule representing a nick in double-stranded DNA. These results identifying interaction of PARP-1 with a BER intermediate are discussed in light of PARP-1's role in mammalian BER.

Lavrik, Olga I.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Prasad, Rajendra (National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Sobol, Robert W.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Horton, Julie K.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences; Ackerman, Eric J.(BATTELLE (PACIFIC NW LAB)) [BATTELLE (PACIFIC NW LAB); Wilson, Samuel H.(National Institute of Environmental Health Sciences) [National Institute of Environmental Health Sciences

2001-07-06T23:59:59.000Z

184

Follicle-Stimulating Hormone Induction of Steel Factor (SLF) mRNA in Mouse Sertoli Cells and Stimulation of DNA Synthesis in Spermatogonia by Soluble SLF  

Science Journals Connector (OSTI)

Follicle-stimulating hormone (FSH) and its intracellular mediator, cAMP, increase the mRNA levels for the Steel factor (SLF, the c-kit ligand) in cultured primary mouse Sertoli cells. The inductive effect of cAMP is more evident in cultures from 13-day-old animals than in cultures from 18-day-old animals. Analysis through the polymerase chain reaction (PCR) indicates that (Bu)2cAMP or FSH treatment increases the levels of the mRNAs for both the potentially soluble form and the transmembrane form of SLF in cultured Sertoli cells. The ratio between mRNAs encoding the potentially soluble form and the transmembrane form of SLF increases during postnatal testis development, and it is higher in cultured Sertoli cells with respect to total testis, suggesting that, under the in vitro conditions, SLF could be produced by Sertoli cells mainly as a soluble factor. Soluble recombinant SLF stimulates, in a dose-dependent fashion, thymidine incorporation in cultures of isolated germ cell populations enriched in the mitotic stages (spermatogonia), independently of the presence of serum, whereas cAMP analogs have no effect. Autoradiographic analysis shows that SLF selectively stimulates DNA synthesis in type A spermatogonia.

Pellegrino Rossi; Susanna Dolci; Cristina Albanesi; Paola Grimaldi; Rossana Ricca; Raffaele Geremia

1993-01-01T23:59:59.000Z

185

COLLEGE OF LIBERAL ARTS AND HUMAN SCIENCES DEPARTMENT OF MUSIC  

E-Print Network [OSTI]

Century Literature 3 MUS 3134 Vocal Music Literature 3 MUS 3144 Early Music Literature 3 MUS 4124 Special Applied Camp. 8 (2) __ (2) __ (2) __ (2) __ MUS 4734 Adv. Indiv. Applied Camp. 8 (2) __ (2)_._ (2) __ (2

Virginia Tech

186

Effects of the PPAR{gamma} agonist troglitazone on endothelial cells in vivo and in vitro: Differences between human and mouse  

SciTech Connect (OSTI)

Peroxisome proliferator-activated receptor gamma (PPAR{gamma}) agonists and PPAR{gamma}/{alpha} dual agonists have been or are being developed for clinical use in the treatment of type 2 diabetes mellitus and hyperlipidemias. A common tumor finding in rodent carcinogenicity studies for these agonists is hemangioma/hemangiosarcoma in mice but not in rats. We hypothesized that increased endothelial cell proliferation may be involved in the mechanism of PPAR agonist-induced vascular tumors in mice, and we investigated the effects on endothelial cells utilizing troglitazone, the first clinically used PPAR{gamma} agonist, in vivo and in vitro. Troglitazone (400 and 800 mg/kg/day) induced hemangiosarcomas in mice in a 2-year bioassay. We showed that troglitazone increased endothelial cell proliferation in brown and white adipose tissue and liver in mice at sarcomagenic doses after 4 weeks of treatment. Troglitazone was cytotoxic both to human dermal microvascular endothelial cells (HMEC1) and mouse mammary fat pad microvascular endothelial cells (MFP MVEC) at high concentrations. However, MFP MVEC were more resistant to the cytotoxic effects of troglitazone based on the much lower LC{sub 50} in HMEC1 (17.4 {mu}M) compared to MFP MVEC (92.2 {mu}M). Troglitazone increased the proliferation and survival of MFP MVEC but not HMEC1 in growth factor reduced conditions. Our data demonstrate that troglitazone may induce hemangiosarcomas in mice, at least in part, through enhancement of survival and proliferation of microvascular endothelial cells. Such an effect does not occur with human cells, suggesting that human may react differently to exposure to PPAR agonists compared with mice.

Kakiuchi-Kiyota, Satoko [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States); Vetro, Joseph A. [Center for Drug Delivery and Nanomedicine and Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198-6025 (United States); Suzuki, Shugo; Varney, Michelle L. [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States); Han, Huai-Yun [Center for Drug Delivery and Nanomedicine and Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE 68198-6025 (United States); Nascimento, Merielen; Pennington, Karen L.; Arnold, Lora L.; Singh, Rakesh K. [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States); Cohen, Samuel M. [Department of Pathology and Microbiology and the Eppley Institute for Cancer Research, University of Nebraska Medical Center, 983135 Nebraska Medical Center Omaha, NE 68198-3135 (United States)], E-mail: scohen@unmc.edu

2009-05-15T23:59:59.000Z

187

Extracellular ATP-induced nuclear Ca{sup 2+} transient is mediated by inositol 1,4,5-trisphosphate receptors in mouse pancreatic {beta}-cells  

SciTech Connect (OSTI)

Extracellular ATP (eATP) induces an intracellular Ca{sup 2+} transient by activating phospholipase C (PLC)-associated P2X4 purinergic receptors, leading to production of inositol 1,4,5-trisphosphate (IP3) and subsequent Ca{sup 2+} release from intracellular stores in mouse pancreatic {beta}-cells. Using laser scanning confocal microscopy, Ca{sup 2+} indicator fluo-4 AM, and the cell permeable nuclear indicator Hoechst 33342, we examined the properties of eATP-induced Ca{sup 2+} release in pancreatic {beta}-cell nuclei. eATP induced a higher nuclear Ca{sup 2+} transient in pancreatic {beta}-cell nuclei than in the cytosol. After pretreatment with thapsigargin (TG), an inhibitor of sarco-endoplasmic reticulum Ca{sup 2+}-ATPase (SERCA) pumps, the amplitude of eATP-induced Ca{sup 2+} transients in the nucleus was still much higher than those in the cytosol. This effect of eATP was not altered by inhibition of either the plasma membrane Ca{sup 2+}-ATPase (PMCA) or the plasma membrane Na{sup +}/Ca{sup 2+} exchanger (NCX) by LaCl{sub 3} or by replacement of Na{sup +} with N-Methyl-Glucosamine. eATP-induced nuclear Ca{sup 2+} transients were abolished by a cell-permeable IP3R inhibitor, 2-aminoethoxydiphenyl borate (2-APB), but were not blocked by the ryanodine receptor (RyR) antagonist ryanodine. Immunofluorescence studies showed that IP3Rs are expressed on the nuclear envelope of pancreatic {beta}-cells. These results indicate that eATP triggers nuclear Ca{sup 2+} transients by mobilizing a nuclear Ca{sup 2+} store via nuclear IP3Rs.

Chen, Zheng; Li, Zhengzheng; Peng, Gong; Chen, Xiaoli; Yin, Wenxuan [National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd., Beijing 100101 (China)] [National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd., Beijing 100101 (China); Kotlikoff, Michael I. [Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853 (United States)] [Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853 (United States); Yuan, Zeng-qiang, E-mail: zqyuan@sun5.ibp.ac.cn [National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd., Beijing 100101 (China)] [National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd., Beijing 100101 (China); Ji, Guangju, E-mail: gj28@ibp.ac.cn [National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd., Beijing 100101 (China)] [National Laboratory of Biomacromolecules, Institute of Biophysics of Chinese Academy of Sciences, 15 Datun Rd., Beijing 100101 (China)

2009-05-01T23:59:59.000Z

188

CHIR99021 promotes self-renewal of mouse embryonic stem cells by modulation of protein-encoding gene and long intergenic non-coding RNA expression  

SciTech Connect (OSTI)

Embryonic stem cells (ESCs) can proliferate indefinitely in vitro and differentiate into cells of all three germ layers. These unique properties make them exceptionally valuable for drug discovery and regenerative medicine. However, the practical application of ESCs is limited because it is difficult to derive and culture ESCs. It has been demonstrated that CHIR99021 (CHIR) promotes self-renewal and enhances the derivation efficiency of mouse (m)ESCs. However, the downstream targets of CHIR are not fully understood. In this study, we identified CHIR-regulated genes in mESCs using microarray analysis. Our microarray data demonstrated that CHIR not only influenced the Wnt/?-catenin pathway by stabilizing ?-catenin, but also modulated several other pluripotency-related signaling pathways such as TGF-?, Notch and MAPK signaling pathways. More detailed analysis demonstrated that CHIR inhibited Nodal signaling, while activating bone morphogenetic protein signaling in mESCs. In addition, we found that pluripotency-maintaining transcription factors were up-regulated by CHIR, while several developmental-related genes were down-regulated. Furthermore, we found that CHIR altered the expression of epigenetic regulatory genes and long intergenic non-coding RNAs. Quantitative real-time PCR results were consistent with microarray data, suggesting that CHIR alters the expression pattern of protein-encoding genes (especially transcription factors), epigenetic regulatory genes and non-coding RNAs to establish a relatively stable pluripotency-maintaining network. - Highlights: • Combined use of CHIR with LIF promotes self-renewal of J1 mESCs. • CHIR-regulated genes are involved in multiple pathways. • CHIR inhibits Nodal signaling and promotes Bmp4 expression to activate BMP signaling. • Expression of epigenetic regulatory genes and lincRNAs is altered by CHIR.

Wu, Yongyan [College of Veterinary Medicine, Northwest A and F University, Yangling 712100, Shaanxi (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling 712100, Shaanxi (China); Ai, Zhiying [Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling 712100, Shaanxi (China); College of Life Sciences, Northwest A and F University, Yangling 712100, Shaanxi (China); Yao, Kezhen [College of Veterinary Medicine, Northwest A and F University, Yangling 712100, Shaanxi (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling 712100, Shaanxi (China); Cao, Lixia; Du, Juan; Shi, Xiaoyan [Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling 712100, Shaanxi (China); College of Life Sciences, Northwest A and F University, Yangling 712100, Shaanxi (China); Guo, Zekun, E-mail: gzk@nwsuaf.edu.cn [College of Veterinary Medicine, Northwest A and F University, Yangling 712100, Shaanxi (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling 712100, Shaanxi (China); Zhang, Yong, E-mail: zhylab@hotmail.com [College of Veterinary Medicine, Northwest A and F University, Yangling 712100, Shaanxi (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling 712100, Shaanxi (China)

2013-10-15T23:59:59.000Z

189

The diabetogenic mouse MHC class II molecule I-A[subscript g7] is endowed with a switch that modulates TCR affinity  

SciTech Connect (OSTI)

Genetic susceptibility to autoimmunity is frequently associated with specific MHC alleles. Diabetogenic MHC class II molecules, such as human HLA-DQ8 and mouse I-A{sub g7}, typically have a small, uncharged amino acid residue at position 57 of their {beta} chain ({beta}57); this results in the absence of a salt bridge between {beta}57 and Arg{alpha}76, which is adjacent to the P9 pocket of the peptide-binding groove. However, the influence of Arg{alpha}76 on the selection of the TCR repertoire remains unknown, particularly when the MHC molecule binds a peptide with a neutral amino acid residue at position P9. Here, we have shown that diabetogenic MHC class II molecules bound to a peptide with a neutral P9 residue primarily selected and expanded cells expressing TCRs bearing a negatively charged residue in the first segment of their complementarity determining region 3{beta}. The crystal structure of one such TCR in complex with I-A{sub g7} bound to a peptide containing a neutral P9 residue revealed that a network of favorable long-range (greater than 4 {angstrom}) electrostatic interactions existed among Arg{alpha}76, the neutral P9 residue, and TCR, which supported the substantially increased TCR/peptide-MHC affinity. This network could be modulated or switched to a lower affinity interaction by the introduction of a negative charge at position P9 of the peptide. Our results support the existence of a switch at residue {beta}57 of the I-Ag7 and HLA-DQ8 class II molecules and potentially link normal thymic TCR selection with abnormal peripheral behavior.

Yoshida, Kenji; Corper, Adam L.; Herro, Rana; Jabri, Bana; Wilson, Ian A.; Teyton, Luc (Scripps); (UC)

2011-11-16T23:59:59.000Z

190

The diabetogenic mouse MHC class II molecule I-A[superscript g7] is endowed with a switch that modulates TCR affinity  

SciTech Connect (OSTI)

Genetic susceptibility to autoimmunity is frequently associated with specific MHC alleles. Diabetogenic MHC class II molecules, such as human HLA-DQ8 and mouse I-A{sup g7}, typically have a small, uncharged amino acid residue at position 57 of their {beta} chain ({beta}57); this results in the absence of a salt bridge between {beta}57 and Arg{alpha}76, which is adjacent to the P9 pocket of the peptide-binding groove. However, the influence of Arg{alpha}76 on the selection of the TCR repertoire remains unknown, particularly when the MHC molecule binds a peptide with a neutral amino acid residue at position P9. Here, we have shown that diabetogenic MHC class II molecules bound to a peptide with a neutral P9 residue primarily selected and expanded cells expressing TCRs bearing a negatively charged residue in the first segment of their complementarity determining region 3{beta}. The crystal structure of one such TCR in complex with I-Ag7 bound to a peptide containing a neutral P9 residue revealed that a network of favorable long-range (greater than 4 {angstrom}) electrostatic interactions existed among Arg{alpha}76, the neutral P9 residue, and TCR, which supported the substantially increased TCR/peptide-MHC affinity. This network could be modulated or switched to a lower affinity interaction by the introduction of a negative charge at position P9 of the peptide. Our results support the existence of a switch at residue {beta}57 of the I-A{sup g7} and HLA-DQ8 class II molecules and potentially link normal thymic TCR selection with abnormal peripheral behavior.

Yoshida, Kenji; Corper, Adam L.; Herro, Rana; Jabri, Bana; Wilson, Ian A.; Teyton, Luc (Scripps); (UC)

2010-07-22T23:59:59.000Z

191

Evaluation of a bioluminescent mouse model expressing aromatase PII-promoter-controlled luciferase as a tool for the study of endocrine disrupting chemicals  

SciTech Connect (OSTI)

Dysfunction of the enzyme aromatase (CYP19) is associated with endocrine pathologies such as osteoporosis, impaired fertility and development of hormone-dependent cancers. Certain endocrine disrupting chemicals affect aromatase expression and activity in vitro, but little is known about their ability to do so in vivo. We evaluated a bioluminescent mouse model (LPTA (registered)) CD-1-Tg(Cyp19-luc)-Xen) expressing luciferase under control of the gonadal aromatase pII promoter as an in vivo screening tool for chemicals that may affect aromatase expression. We studied the effects of forskolin, pregnant mare serum gonadotropin and atrazine in this model (atrazine was previously shown to induced pII-promoter-driven aromatase expression in H295R human adrenocortical carcinoma cells). About 2-4 out of every group of 10 male or female Cyp19-luc mice injected i.p. with 10 mg/kg forskolin had increased gonadal bioluminescence after 3-5 days compared to controls; the others appeared non-responsive. Similarly, about 4 per group of 9 individual females injected with pregnant mare serum gonadotropin had increased ovarian bioluminescence after 24 h. There was a statistically significant correlation between ovarian bioluminescence and plasma estradiol concentrations (n = 14; p = 0.022). Males exposed to a single dose of 100 mg/kg or males and females exposed to 5 daily injections of 30 mg/kg atrazine showed no change in gonadal bioluminescence over a 7 day period, but a significant interaction was found between atrazine (100 mg/kg) and time in female mice (p < 0.05; two-way ANOVA). Ex vivo luciferase activity in dissected organs was increased by forskolin in testis, epididymis and ovaries. Atrazine (30 mg/kg/day) increased (30%) luciferase activity significantly in epididymis only. In conclusion, certain individual Cyp19-luc mice are highly responsive to aromatase inducers, suggesting this model, with further optimization, may have potential as an in vivo screening tool for environmental contaminants.

Rivest, Patricia, E-mail: patricia.rivest@iaf.inrs.ca; Devine, Patrick J., E-mail: patrick.devine@iaf.inrs.ca; Sanderson, J. Thomas, E-mail: thomas.sanderson@iaf.inrs.c

2010-11-15T23:59:59.000Z

192

Developmental defects and genomic instability after x-irradiation of wild-type and genetically modified mouse pre-implantation and early post-implantation embryos  

Science Journals Connector (OSTI)

Results obtained from the end of the 1950s suggested that ionizing radiation could induce foetal malformations in some mouse strains when administered during early pre-implantation stages. Starting in 1989, data obtained in Germany also showed that radiation exposure during that period could lead to a genomic instability in the surviving foetuses. Furthermore, the same group reported that both malformations and genomic instability could be transmitted to the next generation foetuses after exposure of zygotes to relatively high doses of radiation. As such results were of concern for radiation protection, we investigated this in more detail during recent years, using mice with varying genetic backgrounds including mice heterozygous for mutations involved in important cellular processes like DNA repair, cell cycle regulation or apoptosis. The main parameters which were investigated included morphological development, genomic instability and gene expression in the irradiated embryos or their own progeny. The aim of this review is to critically reassess the results obtained in that field in the different laboratories and to try to draw general conclusions on the risks of developmental defects and genomic instability from an exposure of early embryos to moderate doses of ionizing radiation. Altogether and in the range of doses normally used in diagnostic radiology, the risk of induction of embryonic death and of congenital malformation following the irradiation of a newly fertilised egg is certainly very low when compared to the 'spontaneous' risks for such effects. Similarly, the risk of radiation induction of a genomic instability under such circumstances seems to be very small. However, this is not a reason to not apply some precaution principles when possible. One way of doing this is to restrict the use of higher dose examinations on all potentially pregnant women to the first ten days of their menstrual cycle when conception is very unlikely to have occurred (the so-called ten-day rule), as already recommended by the Health Protection Agency. Such a precautionary attitude would also be supported by the uncertainties associated with later changes in gene expression which might result from irradiation or early embryos with moderate doses.

P Jacquet

2012-01-01T23:59:59.000Z

193

Two distinct endogenous type C viruses isolated from the asian rodent Mus cervicolor: conservation of virogene sequences in related rodent species.  

Science Journals Connector (OSTI)

...tumor virus; Rad-LV, radiation leukemia virus. bThese...BENVENISTE ET AL. The detection of virogene sequences...J. Todaro. 1974. Detection of ba- boon type C...activity of filtrates from radiation induced lymphoid tumors...RD-114/CCC group and detection of antigen in normal...

R E Benveniste; R Callahan; C J Sherr; V Chapman; G J Todaro

1977-03-01T23:59:59.000Z

194

Levels of the Rab GDP dissociation inhibitor (GDI) are altered in the prenatal restrain stress mouse model of schizophrenia and are differentially regulated by the mGlu2/3 receptor agonists, LY379268 and LY354740  

Science Journals Connector (OSTI)

Abstract LY379268 and LY354740, two agonists of mGlu2/3 metabotropic glutamate receptors, display different potencies in mouse models of schizophrenia. This differential effect of the two drugs remains unexplained. We performed a proteomic analysis in cultured cortical neurons challenged with either LY379268 or LY354740. Among the few proteins that were differentially influenced by the two drugs, Rab GDP dissociation inhibitor-? (Rab GDI?) was down-regulated by LY379268 and showed a trend to an up-regulation in response to LY354740. In cultured hippocampal neurons, LY379268 selectively down-regulated the ? isoform of Rab GDI. Rab GDI inhibits the activity of the synaptic vesicle-associated protein, Rab3A, and is reduced in the brain of schizophrenic patients. We examined the expression of Rab GDI in mice exposed to prenatal stress (“PRS mice”), which have been described as a putative model of schizophrenia. Rab GDI? protein levels were increased in the hippocampus of PRS mice at postnatal days (PND)1 and 21, but not at PND60. At PND21, PRS mice also showed a reduced depolarization-evoked [3H]d-aspartate release in hippocampal synaptosomes. The increase in Rab GDI? levels in the hippocampus of PRS mice was reversed by a 7-days treatment with LY379268 (1 or 10 mg/kg, i.p.), but not by treatment with equal doses of LY354740. These data strengthen the validity of PRS mice as a model of schizophrenia, and show for the first time a pharmacodynamic difference between LY379268 and LY354740 which might be taken into account in an attempt to explain the differential effect of the two drugs across mouse models.

Rosamaria Orlando; Marina Borro; Marta Motolese; Gemma Molinaro; Sergio Scaccianoce; Alessandra Caruso; Luigi di Nuzzo; Filippo Caraci; Francesco Matrisciano; Anna Pittaluga; Jerome Mairesse; Maurizio Simmaco; Robert Nisticò; James A. Monn; Ferdinando Nicoletti

2014-01-01T23:59:59.000Z

195

Mouse Strain-Dependent Variations in Sensitivity to Induction of Gamma-H2AX Foci after Continuous Low Dose-Rate Irradiation: The Atm-/- vs Atm +/+ genotypes on Balb/c, 129S6, C57BL/6J, and A/J inbred strains  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mouse Strain-Dependent Variations in Sensitivity to Induction of Gamma-H2AX Foci Mouse Strain-Dependent Variations in Sensitivity to Induction of Gamma-H2AX Foci after Continuous Low Dose-Rate Irradiation: The Atm-/- vs Atm +/+ genotypes on Balb/c, 129S6, C57BL/6J, and A/J inbred strains. J.R.Brogan 1 , H. Nagasawa 1 , T.A Kato 1 , C.Fallgren 1 , T.Wade 1 . P.Genik 1 , J.B.Little 2 , J.S.Bedford 1 , and M.M.Weil 1 1 Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523; 2 Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston MA 02115 We have recently developed a 'low dose rate' gamma-H2AX assay that is sufficiently sensitive to distinguish differences in response for cells from normal Atm +/+ (mouse) or ATM+/+ (human) and the phenotypes associated with the corresponding

196

Gamma irradiation of the prenatal mouse dentition  

E-Print Network [OSTI]

as the dental lamina to the stage of the deposition of enamel and dentin. The purpose of this study was to determine the effect of a continuous stress of gamma irradiation on the structure of the odontogenic cells, the relative size and rate of development... development. In 1927, Leist (9) made a study of the effect of X-rays on teeth, which was brought about by the following rase. A worker in a Roentgen tube factory was exposed daily to a considerable dose of X-irradiation. Sometime later he began to show...

Kerley, Michael Auston

1969-01-01T23:59:59.000Z

197

Cellular fusion in the mouse intestine.  

E-Print Network [OSTI]

??Tissue regeneration after injury poses a major challenge that requires the fine balance between stimulation and dysregulation of cell proliferation in order to facilitate homeostatic… (more)

Powell, Anne Elizabeth

2010-01-01T23:59:59.000Z

198

Regression Kinetics of Mouse Skin Papillomas  

Science Journals Connector (OSTI)

...stronger tendency to regress. Roe et al. (8) have published...Skocik. APRIL 1976 1425 F. J. Burns et a!. REFERENCES 1. Berenblum...40: 823-846, 1968. 8. Roe, F. J. C., Carter, R...9. Salaman, M. H., and Roe, R. J. C. Carcinogenesis...

Fredric J. Burns; Martin Vanderlaan; Andrew Sivak; and Roy E. Albert

1976-04-01T23:59:59.000Z

199

Bol. Mus. Para. Emlio Goeldi. Cincias Humanas, Belm, v. 3, n. 2, p. 195-211, maio-ago. 2008 A cidade, um foco de diversidade agrcola no Rio Negro (Amazonas, Brasil)?  

E-Print Network [OSTI]

A cidade, um foco de diversidade agrícola no Rio Negro (Amazonas, Brasil)? The urban area, a center pour le Développement. Unité de Recherche 200, Brasília, Brasil (laura.emperaire@uol.com.br). II;A cidade, um foco de diversidade agrícola no Rio Negro (Amazonas, Brasil)? 196 INTRODU��O O

Paris-Sud XI, Université de

200

E-Print Network 3.0 - antigen gene family Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

similar to open reading frame M.musculus ... Source: Leng, Chenlei - Department of Statistics and Applied Probability, National University of Singapore Collection:...

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

E-Print Network 3.0 - antigen family gene Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

similar to open reading frame M.musculus ... Source: Leng, Chenlei - Department of Statistics and Applied Probability, National University of Singapore Collection:...

202

Heavy-Duty Low-Temperature and Diesel Combustion & Heavy-Duty...  

Broader source: Energy.gov (indexed) [DOE]

Musculus 425 RelevanceObjectives: H-D In-Cylinder Combustion Current Objectives: SNL - Spatialtemporal evolution of LTC soot-precursors SNL - Injector dribble effects on UHC ...

203

An In-Cylinder Imaging Survey of Low-Temperature, High-Efficiency...  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

luminosity of low temperature combustion strategies are contrasted to conventional gasoline and diesel engine combustion deer11musculus.pdf More Documents & Publications...

204

Cleaner, More Efficient Diesel Engines  

SciTech Connect (OSTI)

Mark Musculus, an engine combustion scientist at Sandia National Laboratories, led a study that outlines the science base for auto and engine manufacturers to build the next generation of cleaner, more efficient engines using low-temperature combustion. Here, Musculus discusses the work at Sandia's Combustion Research Facility.

Musculus, Mark

2013-08-13T23:59:59.000Z

205

Expression of esterase-2 in developing mouse embryos and neonates  

E-Print Network [OSTI]

as maternal invironments in which the gene product can be assayed. The esterase-2 gene product (ES-2) was purified by means of isoelectric foc- using and gel filtration of C57BL/6J kidney homogenates. Purified ES-2 was used to elicit antisera from New... in kidney. . Page 28 LIST OF FIGURES Page 1. Heat curves. 14 2. Immunoelectrophoretic rockets of purified ES-2. 15 3. Rocket areas vs. purified ES-2. 16 4. Starch gel of purified ES-2 18 5. Crossed electrophoresis 19 6. Immunoelectrophoretic...

Deal, Frank Hans

1981-01-01T23:59:59.000Z

206

Static and dynamic tactile directional cues experiments with VTPlayer mouse  

E-Print Network [OSTI]

, is a track- ball: the braille cell replaces the scrolling wheel. The second one is called Tacti] added a 2Ã?4 braille cell on three devices to add tactile information. The first one, called TactiBall

Paris-Sud XI, Université de

207

Ethanol Promotes Intestinal Tumorigenesis in the MIN Mouse  

Science Journals Connector (OSTI)

Short Communications Ethanol Promotes Intestinal Tumorigenesis in...Twenty-four MIN mice were randomized to ethanol supplementation in the drinking water...a mutagenic adduct associated with ethanol consumption. Ethanol supplementation...

Hemant K. Roy; James M. Gulizia; William J. Karolski; Anne Ratashak; Michael F. Sorrell; Dean Tuma

2002-11-01T23:59:59.000Z

208

The evaluation of add-on mouse pads using electromyography  

E-Print Network [OSTI]

cori teat e terieott as seen in Table 2, however, this was as a result of the gender by treatment interaction, meaning the genders responded differently to the treatments as seen in Table 3. TABLE 2 Time F value (Hrs'l 0 048 2 182 4 198 6 418... Overall Treatment Effects on the Trapezius Pr & F Significance 0 7077 ns 02441 ni 0 2178 ns 0 0643 ns TABLE 3 Time F value (Hts) 0 93 1 69 762 5 77 Gender by Treatment Effects on the Trapezius Pr & F Significance 0 4812 ns 0 2673 ns 0...

Thomas, Varghese

2012-06-07T23:59:59.000Z

209

Mouse immunoglobulin D: messenger RNA and genomic DNA sequences  

Science Journals Connector (OSTI)

...a-helical or a, 8-pleated sheet conformation. Very similar...0.971.912 0.973, 8-Sheet potential (Ps) 1.045 1...phase; AG is the change in free energy in kilo-calories per mole...will initiate, 8-pleated sheet formations; values > 1.000...

PW Tucker; CP Liu; JF Mushinski; FR Blattner

1980-09-19T23:59:59.000Z

210

RAPID/Best Practices/Memorandums of Understanding (MOUs) for...  

Open Energy Info (EERE)

In some cases, the development of an MOU (particularly negotiation of the MOU terms) has been viewed as a distraction that prevented the project from move forward in a...

211

The development of direction selectivity in the mouse retina  

E-Print Network [OSTI]

firing of starburst amacrine cells and DSGCs, is not necessary to establish the retinal circuitsfiring rate in the null direction is as low as in adult, indicating that the circuits

Elstrott, Justin Blake

2009-01-01T23:59:59.000Z

212

Characterization of Neuropeptide Y Expressing Cells in the Mouse Retina  

E-Print Network [OSTI]

present in two layers, the inner nuclear layer (INL) and the ganglion cell layer (GCL). The cells in both layers were densely distributed, with those in the INL having a mean density of 1452 65 cells/mm2 , and those in the GCL having a mean density of 644 41 cells/mm2 . The cells in the INL extended

Nirenberg, Sheila

213

Control of Adenosine Monophosphate Catabolism in Mouse Ascites Tumor Cells  

Science Journals Connector (OSTI)

...relationship was noted between the energy charge value and the rate of...Animals Deoxyglucose pharmacology Energy Metabolism drug effects Glucose...phosphate and the adenine nucleo- tides were measured at several time...intracellular AMP, decreased the energy charge, and either decreased...

Leonard A. Sauer

1978-04-01T23:59:59.000Z

214

Laminar circuit organization and response modulation in mouse visual cortex  

E-Print Network [OSTI]

1.25 NaH2PO4, 4 MgCl2, 0.5 CaCl2, and 24 NaHCO3). SlicesNaCl, 2.5 KCl, 26 NaHCO3, 2 CaCl2, 2 MgCl2, 1.25 NaH2PO4,

Olivas, Nicholas D; Quintanar-Zilinskas, Victor; Nenadic, Zoran; Xu, Xiangmin

2012-01-01T23:59:59.000Z

215

Observations on feeding behavior in the vesper mouse, Nyctomys sumichrasti  

E-Print Network [OSTI]

47C119C119C119C46C117C114C98C97C110C102C105C115C99C104C101C114C46C100C101C47C106C111C117C114C110C97C108C115C47C109C97C109C109C98C105C111C108 C90C101C105C116C115C99C104C114C105C102C116 C102C117C200C114C83C97C200C117C103C101C116C105C101C114C107C117C110C... C97C115C44 C76C97C119C114C101C110C99C101C44 C75C97C110C115C97C115C59C68C101C112C97C114C116C109C101C110C116 C111C102C70C111C114C101C115C116C114C121C44C77C105C99C104C105C103C97C110C83C116C97C116C101 C85C110C105C118C101C114C115C105C116C121C44C69C97C115C...

Timm, Robert M.; Vriesendorp, Corine

2003-04-01T23:59:59.000Z

216

Morphine sulfate stimulates the adenylate cyclase in mouse caudate nuclei  

Science Journals Connector (OSTI)

...narcotics and the endogenous opioid polypeptides. This work was supported by U.S. National Institutes of Health Grant NS 12776, the late Mrs. Jean Mauze, the Charles E. Merrill Trust, the American Parkinson's Disease Association, and Mrs. Katherine...

L C Tang; G C Cotzias

1978-01-01T23:59:59.000Z

217

Glove Mouse Project The goal of this project  

E-Print Network [OSTI]

that is cost-effective,easy to use, and portable. Hybrid, High-performance Cluster Computer Setup The objective it to create a viable fuel for use in a diesel engine. Multi-Functional Automated Turret Stand alone turret Design and implementation of an unmanned aerial vehicle which is capable of autonomous control via

de Lijser, Peter

218

Substrate reduction therapy in mouse models of the glycosphingolipidoses  

Science Journals Connector (OSTI)

...Neufeld 1991). The substrate for the defective enzyme accumulates in the lysosome and...progressively accumulates, leading to renal and car- diovascular disease in hemizygous males...M1)-ganglioside accumulation in mice defective in lysosomal acid beta- galactosidase...

2003-01-01T23:59:59.000Z

219

Developmental transitions of germ cell lineage of the mouse  

E-Print Network [OSTI]

Mammalian germ cells arise during early embryogenesis and migrate to the developing gonad where, under the direction of the somatic environment, they initiate distinct sex-specific developmental programs resulting in the ...

Baltus, Andrew Edmund

2006-01-01T23:59:59.000Z

220

The organizer factors Chordin and Noggin are required for mouse  

E-Print Network [OSTI]

by distinctly separated cell populations1,2 . The head organizer is located in the anterior visceral endoderm (AVE) and the trunk organizer in the node and anterior primitive streak. In amphibians, Spemann's organi- zer, which is homologous to the node, partially overlaps with anterior endoderm cells expressing

De Robertis, Eddy M.

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

The proteome of mouse brain microvessel membranes and basal lamina  

E-Print Network [OSTI]

IPI00849927 IPI00113377 Gene Vim Mbp Lrp1 Gnaq Ahnak AlcamIPI00319830.7 IPI00115564.5 Aco2 Vim Tubb3 Ncam1 Gnao1 Mdh2

Chun, Hyun Bae

2011-01-01T23:59:59.000Z

222

C3Bio.org - Tags: wireless mouse  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

The Great Pyramid is found in... - Select an answer - Egypt Japan Canada Rocks The North Pole Please leave this field blank. required Problem: required Attach a screenshot:...

223

Interferon-Stimulated Genes in the Pregnant Mouse Uterus  

E-Print Network [OSTI]

on for eac h respe ctive g ene. L E, luminal e pithel ium; G E, glan dula r e pitheli um; D ,d eci du a. D D D D LE LE LE GE D LE 18 FIG. 4. ISH analysis of Isg1 5, Nm i, O as2 , a nd Plsc r1 in e ndom etria an d con ceptuse... GR: Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 1994, 264(5164):1415- 1421. 27. Ritchie KJ, Hahn CS, Kim KI, Yan M, Rosario D, Li L, de la Torre JC, Zhang DE: Role of ISG...

Tilford, Sarah

2008-08-24T23:59:59.000Z

224

A Systematic Analysis of a Deep Mouse Epididymal Sperm Proteome...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

energy production via glycolysis, ?-oxidation and oxidative phosphorylation, protein folding and transport, and cell signaling systems. This proteome should prove a useful...

225

ISOLATION AND CHARACTERISATION OF SYNAPTIC VESICLES FROM MOUSE BRAIN  

E-Print Network [OSTI]

vesicles 64 3.6.2 Synaptic vesicle fusion 65 3.7 Analysis of subcellular fractions from Rab-GDI 1 KO mice 75 4.4 Effects of Rab-GDI1 deletion o

Jahn, Reinhard

226

Neurogenomics in the mouse model : multivariate statistical methods and analyses  

E-Print Network [OSTI]

Irwin, Inc. , Illinois. Rademakers R, Sleegers K, Theuns J,2001; Melchor et al. 2003; Rademakers et al. 2005; Jacobs et

Zapala, Matthew Alan

2007-01-01T23:59:59.000Z

227

Transgenic mouse models of childhood-onset psychiatric disorders  

E-Print Network [OSTI]

Childhood-onset psychiatric disorders, such as attention deficit hyperactivity disorder (ADHD), autism spectrum disorder (ASD), mood disorders, obsessive compulsive spectrum disorders (OCSD), and schizophrenia (SZ), affect ...

Robertson, Holly Rochelle

228

Chloramphenicol: Effects on Mouse Myeloma Cells in Tissue Culture  

Science Journals Connector (OSTI)

...SUSPENSION CULTURE, EXPERIMENTAL CELL RESEARCH 47 : 608 ( 1967 ). SAIDI, P, EFFECT OF CHLORAMPHENICOL ON ERYTHROPOIESIS, JOURNAL...Medicine, St. Louis, Missouri 63110 References and Notes 1. P. Saidi, R. 0. Wallerstein, P. M. Aggeler, J. Lab. Clin. Med...

Boyd K. Hartman; Olive S. Pettengill; George D. Sorenson

1969-07-18T23:59:59.000Z

229

Nicotinamide and Nicotinamide Analogues as Antitumor Promoters in Mouse Skin  

Science Journals Connector (OSTI)

...1 microg of 1alpha,25(OH)2D3 was applied 30 min before 5 microg of TPA, the times...1alpha,25(OH)2D3 at 1 microg was applied at Stage I + II or Stage II, tumor formation...out by an experiment in which TPA was applied to the 1alpha,25(OH)2D3 alone-treated...

Andreas Ludwig; Manfred Dietel; Gity Schäfer; Karin Müller; and Helmuth Hilz

1990-04-15T23:59:59.000Z

230

Sodium Transport in a Mouse Model of Colonic Carcinogenesis  

Science Journals Connector (OSTI)

...transport changes in the colonie mucosa of car cinogen-treated CFi mice after 4 weeks...9 1.0Electric potential in mV. ' Conductance in mS...ratios in human cancer cells as revealed by energy dispersive X-ray microanalysis. J...

Richard J. Davies; Wibo F. Weidema; Geoffrey I. Sandle; Lawrence Palmer; Eleanor E. Deschner; and Jerome J. DeCosse

1987-09-01T23:59:59.000Z

231

Monitoring transient repolarization segment morphology deviations in mouse ECG  

E-Print Network [OSTI]

This thesis details the design, implementation and validation of a system that facilitates partial automation for detection of anomalous repolarization segment morphologies in the ECG of mice. The technology consists of ...

Oefinger, Matthew Blake, 1976-

2006-01-01T23:59:59.000Z

232

Amplification and novel locations of endogenous mouse mammary tumor virus genomes in mouse T-cell lymphomas.  

Science Journals Connector (OSTI)

...except that transfers were hybridized to nick-translated PstI-B env probe. The position...MMTV-a genomes. One hypothesis that merits consideration is that MMTV-a sequences...acid to high specific activity in vitro by nick translation with DNA polymerase I. J...

J Dudley; R Risser

1984-01-01T23:59:59.000Z

233

Relict or colonizer? Extinction and range expansion of penguins in southern New Zealand  

Science Journals Connector (OSTI)

...sampling every 1000th generation and discarding 25 per cent as burn-in. Convergence diagnostics of Bayesian analyses were explored...orientated more anterior-ventrally; sulcus transversus dorsal pit relatively deeper; ventral bit shallower; sulcus tendinis musculus...

2009-01-01T23:59:59.000Z

234

E-Print Network 3.0 - agrodiaetus lepidoptera lycaenidae Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Summary: bner (Lycaenidae, Lepidoptera). Bull. Mus. Comp. Zoo. 101: 479- 540. Nice, C.C. & Shapiro, A.M. 1999... . Molecular and morphological divergence in the butterfly...

235

S1Supplemental Data Serotonin and Go Modulate Functional States  

E-Print Network [OSTI]

derivative of nde-box::YC2.3 (18 nde-box vulval mus- was examined for the presence or absence of Ca2 activity

Schafer, William R.

236

A Congenic Line of the BALB/c Mouse Strain with the Endogenous Mouse Mammary Tumor Virus Proviral Gene Mtv-3: Tissue-specific Expression and Correlation with Resistance to Mouse Mammary Tumor Virus Infection and Tumorigenesis  

Science Journals Connector (OSTI)

...N-methyl-N-nitro-N-nitrosoguanidine by three exposure routes at different developmental...12-dimethylbenzanthracene by two exposure routes at different developmental...human rhabdomyosarcoma cell lines and tumour samples. J Pathol...pancreatic origin for the tumor. Transmission electron microscopy revealed...

Pierre Hainaut; Marc Castellazzi; Dominique Gonzales; Nathalie Clausse; Jo Hilgers; and Michel Crépin

1990-06-15T23:59:59.000Z

237

Protocol: Mouse Cardiac CINE Protocol, Page 1 of 2 Author: R.Bussell 8/9/2013 Protocol: Mouse Cardiac CINE Protocol  

E-Print Network [OSTI]

monitor. The leads should be twisted along their entire length and never form a loop. Tape leads securely the respiratory bellows between the animal and a hard surface so the respiratory signal is reliable. Set the bladder below the diaphragm. If the bladder is too high you may observe the heart beat in the respiratory

Squire, Larry R.

238

Inactivation of the Human Fragile Histidine Triad Gene at 3p14.2 in Monochromosomal Human/Mouse Microcell Hybrid-derived Severe Combined Immunodeficient Mouse Tumors  

Science Journals Connector (OSTI)

...782 782 R-ATCTGGAATAAGTACCTAAGGCGC UBE1L L13852 PBE F-TGGTGCTGACTGCTGCAAAG 408 1036 R-CTCTTGACTTCAGTGATAGCC LUCA1 U03056 SHGC-11855 GDB:733936 F-CAGACTGGAATAGTGGCATAAGG 210 210 R-GGAATGAATAAGTCCACATAAAACG PTPRG U46116 WI-11100 GDB:1220992...

Irina D. Kholodnyuk; Anna Szeles; Ying Yang; George Klein; Stefan Imreh

2000-12-15T23:59:59.000Z

239

Massively parallel sequencing of the mouse exome to accurately identify rare, induced mutations: an immediate source for thousands of new mouse models  

Science Journals Connector (OSTI)

...T A/A A/A yes 3 Olfr1253 MK 0.988 3 19910826 het C/C...T A/A A/T yes 3 Osgin2 MK 0.045 4 140271822 het A/A...Solution hybrid selection with ultra-long oligonucleotides for massively...10)77015-4 ) 12 Boles, MK , 2009 Discovery of candidate...

2012-01-01T23:59:59.000Z

240

Boise State University Evaluation for Graduation  

E-Print Network [OSTI]

Principles of Microeconomics Area II core course in history 3 3 3 3 Area III MATH 124 Introduction of Business 3 3 ITM 104 Operating Systems and Word Processing ITM 105 Spreadsheet Topics ITM 106 Database Music History and Literature II MUS 351 Music History and Literature I OR MUS 353 Music History

Barrash, Warren

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Boise State University Evaluation for Graduation  

E-Print Network [OSTI]

ECON 202 Principles of Microeconomics Area II core course in history 3 3 3 3 Area III MATH 124 Database Topics ITM 310 Business Intelligence 1 1 1 3 MGMT 301 Leadership Skills 3 MKTG 301 Principles Music History and Literature II MUS 351 Music History and Literature I OR MUS 353 Music History

Barrash, Warren

242

Name ID# Date General Degree Credit Requirements  

E-Print Network [OSTI]

core course in history 3 3 3 3 Area III MATH 124 Introduction to Mathematical Thought OR MATH 124 Database Topics ITM 310 Business Intelligence 1 1 1 3 MGMT 301 Leadership Skills 3 MKTG 301 Principles Music History and Literature II MUS 351 Music History and Literature I OR MUS 353 Music History

Barrash, Warren

243

Understanding and preserving nature THE MUSUM THROUGH  

E-Print Network [OSTI]

and anatomy courses, in French (not Latin) 1739 - Georges-Louis Leclerc, Count de Buffon, named Intendant - Musée de l'Homme reopens #12;Welcome to the French Muséum national d'Histoire naturelle. Like nature and innovations, professions and collections, knowledge sharing and partnerships, are broad in scope

244

A mouse model of osteochondromagenesis from clonal inactivation of Ext1 in chondrocytes  

E-Print Network [OSTI]

Lake City, UT 84112; b Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, University of Utah, Salt Lake City, UT 84112 Edited by Neal G. Copeland, Institute of Molecular and Cell. Jonesa,b,1,2 , Virginia Piomboc,1 , Charles Searbyd , Gail Kurrigerb , Baoli Yange , Florian Grabellusf

Capecchi, Mario R.

245

DNA synthetic phase duration of mouse ileal epithelium in acute renal failure  

Science Journals Connector (OSTI)

The effect of progressive acute renal failure on the DNA synthetic phase duration of ileal epithelium was assessed...3-thymidine approximately 18 hours after urinary outflow obstruction in mice. Controls were sub...

Francis T. McDermott; Melinda K. Dalton

246

The effects of triclosan on pluripotency factors and development of mouse embryonic stem cells and zebrafish  

Science Journals Connector (OSTI)

Triclosan (TCS) poses potential risks to reproduction...Sox2 mRNA were not changed, the mRNA levels of Oct4 and Nanog in TCS-treated groups were significantly decreased compared to controls. In addition, the prot...

Xiaojiao Chen; Bo Xu; Xiumei Han; Zhilei Mao; Minjian Chen…

2014-05-01T23:59:59.000Z

247

A Comparison of Glucose Utilization by Mouse Liver and by the Transplantable Hepatocarcinoma C954  

Science Journals Connector (OSTI)

...ways recorded here. In addition, we have investi...Duolite A-3 and C-4 ion-exchange resins, and the water eluates...fully borne out by our demonstration of identical recoveries...can be increased by addition of substrates (glucosone...

S. Abraham; I. L. Chaikoff; and P. Cady

1961-08-01T23:59:59.000Z

248

Progressive Genomic Instability in the FVB/Kras[superscript LA2] Mouse Model of Lung Cancer  

E-Print Network [OSTI]

Alterations in DNA copy number contribute to the development and progression of cancers and are common in epithelial tumors. We have used array Comparative Genomic Hybridization (aCGH) to visualize DNA copy number alterations ...

Jacks, Tyler E.

249

Patterning Mouse and Human Embryonic Stem Cells Using Micro-contact Printing  

E-Print Network [OSTI]

fabricated in a cleanroom facility, template moulds can be repeatedly used to cast PDMS stamps. Further- more

Zandstra, Peter W.

250

Spontaneous Fusion with, and Transformation of Mouse Stroma by, Malignant Human Breast Cancer Epithelium  

Science Journals Connector (OSTI)

...reprobed and analyzed by laser scanning confocal...Because spontaneous fusion between cells of...mechanism. In sum, cell fusion can be an engine...1113-4. 5 Dvorak HF. Tumors: wounds...alterations in the laser-capture-microdissected...Lazebnik Y. Cell fusion: a hidden enemy...

Britta M. Jacobsen; J. Chuck Harrell; Paul Jedlicka; Virginia F. Borges; Marileila Varella-Garcia; and Kathryn B. Horwitz

2006-08-15T23:59:59.000Z

251

Glucose, Lactate, and Lactic Dehydrogenase Activity in Normal Interstitial Fluid and That of Solid Mouse Tumors  

Science Journals Connector (OSTI)

...distant from the tumor site. Warburg, Wind, and Xegelein (21) obtained similar...Sugar, Lactic Acid and CO2-combining Power of Blood Passing through a Tumor. J...Suppl. 154, 1957. 21. WABB oeRO,O.; WIND, F.; and NEGELEIN,E. Ueber den Stoffwechsel...

E. Ann Burgess and B. Sylvén

1962-06-01T23:59:59.000Z

252

Anti-VEGF Antibodies Mitigate the Development of Radiation Necrosis in Mouse Brain  

Science Journals Connector (OSTI)

...patients (17-19 , 22), though these studies lacked statistical power. MRI monitoring of the onset and progression of radiation necrosis...Neurol Neurosurg Psychiatry 1984;47:1338-41. 5. Siu A , Wind JJ, Iorgulescu JB, Chan TA, Yamada Y Sherman JH.Radiation...

Xiaoyu Jiang; John A. Engelbach; Liya Yuan; Jeremy Cates; Feng Gao; Robert E. Drzymala; Dennis E. Hallahan; Keith M. Rich; Robert E. Schmidt; Joseph J.H. Ackerman; and Joel R. Garbow

2014-05-15T23:59:59.000Z

253

A Structurally Based Investigation of Abdominal Aortic Aneurysms in Mouse Models  

E-Print Network [OSTI]

distensible and experienced non-uniform, but modest dilatation. The constitutive parameters reflect elastin loss and increased collagen loading. We detailed the loss of smooth muscle cell contractility as found in human lesions that form in the thoracic aorta...

Collins, Melissa

2012-02-14T23:59:59.000Z

254

E-Print Network 3.0 - arsenic mouse micronucleus Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Environmental Sciences and Ecology ; Geosciences ; Multidisciplinary Databases and Resources 8 Chronic Exposure to Arsenic Causes Increased Cell Survival, DNA Damage, and...

255

Optical Histology: A Method to Visualize Microvasculature in Thick Tissue Sections of Mouse Brain  

E-Print Network [OSTI]

Austin J. Moy1,2 , Matthew P. Wiersma1,2 , Bernard Choi1,2,3 * 1 Beckman Laser Institute, University: Moy AJ, Wiersma MP, Choi B (2013) Optical Histology: A Method to Visualize Microvasculature in Thick

Rose, Michael R.

256

Cryo-electron tomography of mouse hepatitis virus: Insights into the structure of the coronavirion  

Science Journals Connector (OSTI)

...envelope, giving the virions a solar image to which coronaviruses...of CoVs has been the main obstacle to clarifying details...with a Gatan post-column energy filter and a 2k × 2k CCD camera (Gatan). Zero-energy loss imaging was used to...

Montserrat Bárcena; Gert T. Oostergetel; Willem Bartelink; Frank G. A. Faas; Arie Verkleij; Peter J. M. Rottier; Abraham J. Koster; Berend Jan Bosch

2009-01-01T23:59:59.000Z

257

Genome Patterns of Selection and Introgression of Haplotypes in Natural Populations of the House Mouse  

E-Print Network [OSTI]

), homologues of human genes involved in adaptations (e.g. alpha-amylase genes) or in genetic diseases (e

Dean, Matthew D.

258

Effect of Alpha-Amylase on the Ehrlich Mouse Ascites Tumour  

Science Journals Connector (OSTI)

... a water-bath equipped with a shaker following the addition of 25 mgm. of a-amylase (three times recrystallized, Worthington) in 0*5 c.c. water for each 34 ... mortality occurred on about the twelfth day. Mice injected with cell preparations incubated with a-amylase for 3 hr. showed a 40 per cent tumour mortality within 40 days. Increasing ...

F. STECKERL; A. OFODILE; R. R. CAMPBELL; G. H. FRIEDELL

1961-09-09T23:59:59.000Z

259

Establishment and Characterization of a Lethal Mouse Model for the Angola Strain of Marburg Virus  

Science Journals Connector (OSTI)

...tissue in the intestines, kidney, lungs, brain, spleen, and liver. This model provides...including liver, spleen, kidney, lung, brain, and intestine, were also harvested...blood, liver, spleen, kidney, lung, brain, and intestine of each animal. BALB...

Xiangguo Qiu; Gary Wong; Jonathan Audet; Todd Cutts; Yulian Niu; Stephanie Booth; Gary P. Kobinger

2014-08-20T23:59:59.000Z

260

In Vivo Role of Phosphorylation of Cryptochrome 2 in the Mouse Circadian Clock  

Science Journals Connector (OSTI)

...u-tokyo.ac.jp . a Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo...Genetics Research Laboratory, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo...

Arisa Hirano; Nobuhiro Kurabayashi; Tomoki Nakagawa; Go Shioi; Takeshi Todo; Tsuyoshi Hirota; Yoshitaka Fukada

2014-10-06T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

Metabolism and pharmacokinetics of the cyclin-dependent kinase inhibitor R-roscovitine in the mouse  

Science Journals Connector (OSTI)

...hepatic microsomes, plasma, and urine...under an argon atmosphere cooled to 0C was...d6-DMSO, 250 MHz): delta 4...d6-DMSO, 250 MHz): delta 0...at 0C under an atmosphere of argon. When...d6-DMSO, 250 MHz): delta 0...of each). For plasma pharmacokinetic...

Bernard P. Nutley; Florence I. Raynaud; Stuart C. Wilson; Peter M. Fischer; Angela Hayes; Phyllis M. Goddard; Steven J. McClue; Michael Jarman; David P. Lane; and Paul Workman

2005-01-01T23:59:59.000Z

262

Neuroprotective efficacy of aminopropyl carbazoles in a mouse model of amyotrophic lateral sclerosis  

Science Journals Connector (OSTI)

...top of the front paw print. It is evident in...of ALS. Analysis of Plasma, Brain, and Spinal...1 H NMR (CDCl3, 500 MHz) 8.10 (d, 2H...0.13 M) under a N2 atmosphere was cooled in a dry ice...1 H NMR (CDCl3, 400 MHz) 8.23 (d, 2H...

Rachel Tesla; Hamilton Parker Wolf; Pin Xu; Jordan Drawbridge; Sandi Jo Estill; Paula Huntington; LaTisha McDaniel; Whitney Knobbe; Aaron Burket; Stephanie Tran; Ruth Starwalt; Lorraine Morlock; Jacinth Naidoo; Noelle S. Williams; Joseph M. Ready; Steven L. McKnight; Andrew A. Pieper

2012-01-01T23:59:59.000Z

263

The inflammasome promotes adverse cardiac remodeling following acute myocardial infarction in the mouse  

Science Journals Connector (OSTI)

...reflow) occurs in a large number of...tissue in the infarct area composed of tissue...pretreated with gelatin/plasma human-fibronectin...VisualSonics) and a 30-MHz probe as previously...Sigma-Aldrich). The areas of fibrosis and the whole left...a 5% CO2 95% N2 atmosphere in a gas chamber...

Eleonora Mezzaroma; Stefano Toldo; Daniela Farkas; Ignacio M. Seropian; Benjamin W. Van Tassell; Fadi N. Salloum; Harsha R. Kannan; Angela C. Menna; Norbert F. Voelkel; Antonio Abbate

2011-01-01T23:59:59.000Z

264

Metabolism and pharmacokinetics of the cyclin-dependent kinase inhibitor R-roscovitine in the mouse  

Science Journals Connector (OSTI)

...microsomes, plasma, and urine...development from a large set of 2...under an argon atmosphere cooled to...d6-DMSO, 250 MHz): delta...0C under an atmosphere of argon...d6-DMSO, 250 MHz): delta...each). For plasma pharmacokinetic...detected in both plasma or urine...incubations, peak areas of internal...

Bernard P. Nutley; Florence I. Raynaud; Stuart C. Wilson; Peter M. Fischer; Angela Hayes; Phyllis M. Goddard; Steven J. McClue; Michael Jarman; David P. Lane; and Paul Workman

2005-01-01T23:59:59.000Z

265

Epithelial Anion Transporter Pendrin Contributes to Inflammatory Lung Pathology in Mouse Models of Bordetella pertussis Infection  

Science Journals Connector (OSTI)

...Misiak, EC Lavelle, RM McLoughlin, and KH Mills. 2013. Relative contribution of Th1 and...SC , AG Jarnicki, EC Lavelle, and KH Mills. 2006. TLR4 mediates vaccine-induced...Faisy. 2012. Acetazolamide: a second wind for a respiratory stimulant in the intensive...

Karen M. Scanlon; Yael Gau; Jingsong Zhu; Ciaran Skerry; Susan M. Wall; Manoocher Soleimani; Nicholas H. Carbonetti

2014-07-28T23:59:59.000Z

266

Down-regulation of SNAIL suppresses MIN mouse tumorigenesis: Modulation of apoptosis, proliferation, and fractal dimension  

Science Journals Connector (OSTI)

...Foundation grant BES-0238903, General Motors Cancer Research Foundation, and American...AS-PMO optimization has been conducted, future studies will be better able to address...alteration in early precancer. IEEE J Sel Top Quantum Electron 2003;9:243-57. 27 Gurjar...

Hemant K. Roy; Patrick Iversen; John Hart; Yang Liu; Jennifer L. Koetsier; Young Kim; Dhanajay P. Kunte; Madhavi Madugula; Vadim Backman; and Ramesh K. Wali

2004-09-01T23:59:59.000Z

267

Molecular taxonomy of major neuronal classes in the adult mouse forebrain  

E-Print Network [OSTI]

of specific neuronal circuit elements. The mammalian forebrain is a tissue of stunning complexity comprised to which a single canonical circuit comprised of a set of canonical cell types can be recognized across of characteristics including anatomical location, intrinsic firing properties, synaptic physiology, somatodendritic

Cai, Long

268

Hoplopleura janzeni n. sp. (Phthiraptera: Anoplura), a new sucking louse from a Central American swimming mouse  

E-Print Network [OSTI]

HS), 3 dorsal marginal head setae (DMHS), 1 long dorsal principal head seta (DPTS), 1 short dorsal accessory head seta (DAcHS), and 1 dorsal posterior central head seta (DPoCHS) dorsally on each side; 1 DMHS inserted anteriorly and close to dorsal head... 1 lacking setae; sternite 2 with 6 sternal abdominal setae (StAS) with 2 lateral StAS on each side slightly thickened; sternite 3 with 6 StAS with narrow central setae and stout lateral setae (2 on 1 side, 3 on the other); sternites 4–10 each with 6–8...

Durden, Lance A.; Timm, Robert M.

2001-12-01T23:59:59.000Z

269

Use of Course Woody Debris by Cotton Mouse (Peromyscus gossypinus)in a Southeastern Pine Forest  

SciTech Connect (OSTI)

Course woody debris may be an important resource for many small mammals by providing protection and food sources. The author tracked cotton mice movements via radiotelemetry and powder in managed loblolly pine forests. Most day refuges for mice were associated with debris, including rotting stumps (69%), upturned root boles (14%) and under fallen logs (9%). Stumps used were more larger and more highly decomposed. Night time telemetry indicated that mice movements were more closely associated with the logs. Rooting stumps are an important resource for cotton mice.

T.S. McCay

1999-03-22T23:59:59.000Z

270

Genetic programs in human and mouse early embryos revealed by single-cell RNA?sequencing  

Science Journals Connector (OSTI)

... embryos were obtained from the Center for Clinical Reproductive Medicine at the Jiangsu People’s Hospital, Nanjing, China, with patients’ written informed consent and institutional approval. Single-cell isolation ... 26?and?35) at the Center for Clinical Reproductive Medicine at the Jiangsu People’s Hospital, Nanjing, China, with written informed consent and institutional approval. Sperm was obtained from ...

Zhigang Xue; Kevin Huang; Chaochao Cai; Lingbo Cai; Chun-yan Jiang; Yun Feng; Zhenshan Liu; Qiao Zeng; Liming Cheng; Yi E. Sun; Jia-yin Liu; Steve Horvath; Guoping Fan

2013-07-28T23:59:59.000Z

271

Expression of the Wilms' tumor suppressor gene WT1 during mouse embryogenesis  

Science Journals Connector (OSTI)

...ifferential Regulation of the Wilms' Tumor Gene, WTI, during Differentiation of Embryonal Carcinoma...pattern of the Wilms' tumor suppressor gene, WTI, during embryonal development suggests a role for the WTI proteins in the differentiation of specific...

RR Rackley; AM Flenniken; NP Kuriyan; PM Kessler; MH Stoler; BR Williams

1993-12-01T23:59:59.000Z

272

Identification of cis-regulatory elements in mouse Mab21l2 gene by comparative genomics.  

E-Print Network [OSTI]

??The mab-21 gene was first identified in Caenorhabditis elegans as a critical component required for sensory organ identity determination. Mab21 homologs are highly conserved from… (more)

Shek, Kim Fung

2010-01-01T23:59:59.000Z

273

Identification of Distal cis-Regulatory Elements at Mouse Mitoferrin Loci Using Zebrafish Transgenesis  

Science Journals Connector (OSTI)

...immunoprecipitation (ChIP) datasets for the major erythroid transcription...triggers erythroid maturation by nuclear translocation of the fusion...FOG-1, induces an exchange of nuclear factors at both CRMs. GATA-2...immunoprecipitation (ChIP) datasets for the major erythroid transcription...

Julio D. Amigo; Ming Yu; Marie-Berengere Troadec; Babette Gwynn; Jeffrey D. Cooney; Amy J. Lambert; Neil C. Chi; Mitchell J. Weiss; Luanne L. Peters; Jerry Kaplan; Alan B. Cantor; Barry H. Paw

2011-01-19T23:59:59.000Z

274

Analysis of pluripotent mouse stem cell proteomes : insights into post- transcriptional regulation of pluripotency and differentiation  

E-Print Network [OSTI]

nuclear data. False discovery rate is reduced by removing single peptide identifications Measured FDR (%) FDR whole dataset

O'Brien, Robert Norman

2010-01-01T23:59:59.000Z

275

NURBS: a database of experimental and predicted nuclear receptor binding sites of mouse  

Science Journals Connector (OSTI)

......experimental and predicted nuclear receptor binding...experiments. All datasets are processed using...derived from different datasets are comparable...edu 1 INTRODUCTION Nuclear receptors (NRs...experimental and predicted nuclear receptor binding...experiments. All datasets are processed using......

Yaping Fang; Hui-Xin Liu; Ning Zhang; Grace L. Guo; Yu-Jui Yvonne Wan; Jianwen Fang

2013-01-15T23:59:59.000Z

276

E-Print Network 3.0 - accelerated mouse sam Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

decision makers can choose among solar, wind, and Summary: to the National Renewable Energy Laboratory (NREL). NREL has developed a tool--the System Advisor Model (SAM... and...

277

Mitochondrial dysfunction in mouse models of Parkinson’s disease revealed by transcriptomics and proteomics  

E-Print Network [OSTI]

J Bioenerg Biomembr (2009) 41:487–491 DOI 10.1007/s10863-nitrogen species (RNS). J Bioenerg Biomembr (2009) 41:487–MPTP and methamphetamine J Bioenerg Biomembr (2009) 41:487–

Smith, Desmond J.

2009-01-01T23:59:59.000Z

278

Did "Minority Report" Get it Wrong? Superiority of the Mouse over 3D Input Devices  

E-Print Network [OSTI]

-specialists (e.g., Google Earth, Sketchup) has focused increased attention on the problem of improved interaction

Cooperstock, Jeremy R.

279

From pluripotency to differentiation: laying foundations for the body pattern in the mouse embryo  

Science Journals Connector (OSTI)

...genes in ESCs. They review the TGFbeta-related...previously expected. The body plan of the developing embryo...gastrulation stages. They review how regulatory networks constructed...systems. In the final review, Hadjantonakis and co-workers...

2014-01-01T23:59:59.000Z

280

Initiating head development in mouse embryos: integrating signalling and transcriptional activity  

Science Journals Connector (OSTI)

March 2012 review-article Perspective 1001...generation of an embryonic body plan is the outcome of inductive...the embryo acquires its body plan, which manifests as differences...their proper place in the body plan establishes the blueprint of...

2012-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Biophysical Probes of Iron Metabolism in Yeast Cells, Mitochondria, and Mouse Brains  

E-Print Network [OSTI]

Iron is essential in nearly all organisms. It is a cofactor in many proteins and enzymes. This transition metal can also be toxic because it participates in reactions which produce reactive oxygen species. To avoid these toxic effects while still...

Holmes-Hampton, Gregory

2012-10-19T23:59:59.000Z

282

Comparison of trichostatin A and valproic acid treatment regimens in a mouse model of kidney fibrosis  

SciTech Connect (OSTI)

Histone deacetylase (HDAC) inhibitors are promising new compounds for the therapy of fibrotic diseases. In this study we compared the effect of two HDAC inhibitors, trichostatin A and valproic acid, in an experimental model of kidney fibrosis. In mice, doxorubicin (adriamycin) can cause nephropathy characterized by chronic proteinuria, glomerular damage and interstitial inflammation and fibrosis, as seen in human focal segmental glomerulosclerosis. Two treatment regimens were applied, treatment was either started prior to the doxorubicin insult or delayed until a significant degree of proteinuria and fibrosis was present. Pre-treatment of trichostatin A significantly hampered glomerulosclerosis and tubulointerstitial fibrosis, as did the pre-treatment with valproic acid. In contrast, the development of proteinuria was only completely inhibited in the pre-treated valproic acid group, and not in the pre-treated trichostatin A animals. In the postponed treatment with valproic acid, a complete resolution of established doxorubicin-induced proteinuria was achieved within three days, whereas trichostatin A could not correct proteinuria in such a treatment regimen. However, both postponed regimens have comparable efficacy in maintaining the kidney fibrosis to the level reached at the start of the treatments. Moreover, not only the process of fibrosis, but also renal inflammation was attenuated by both HDAC inhibitors. Our data confirm a role for HDACs in renal fibrogenesis and point towards a therapeutic potential for HDAC inhibitors. The effect on renal disease progression and manifestation can however be different for individual HDAC inhibitors. - Highlights: • Valproic acid is a potent antiproteinuric drug, whereas trichostatin A is not. • Trichostatin A and valproic acid reduce kidney fibrosis in doxorubicin nephropathy. • Both valproic acid and trichostatin A attenuate renal inflammation.

Van Beneden, Katrien, E-mail: kvbenede@vub.ac.be [Department of Human Anatomy, Liver Cell Biology Lab, Vrije Universiteit Brussel, Brussels (Belgium); Geers, Caroline [Department of Pathology, Universitair Ziekenhuis Brussel, Brussels (Belgium); Pauwels, Marina [Department of Human Anatomy, Liver Cell Biology Lab, Vrije Universiteit Brussel, Brussels (Belgium); Mannaerts, Inge [Department of Cell Biology, Liver Cell Biology Lab, Vrije Universiteit Brussel, Brussels (Belgium); Wissing, Karl M. [Department of Nephrology, Universitair Ziekenhuis Brussel, Brussels (Belgium); Van den Branden, Christiane [Department of Human Anatomy, Liver Cell Biology Lab, Vrije Universiteit Brussel, Brussels (Belgium); Grunsven, Leo A. van, E-mail: lvgrunsv@vub.ac.be [Department of Cell Biology, Liver Cell Biology Lab, Vrije Universiteit Brussel, Brussels (Belgium)

2013-09-01T23:59:59.000Z

283

E-Print Network 3.0 - apolipoprotein e-deficient mouse Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

17 Research report Summary: , Grauer E, Sehayek E, Michaelson DM. Biochem- ical and cognitive studies of apolipoprotein-E-deficient... . Memory deficits and cholinergic...

284

E-Print Network 3.0 - adult mouse olfactory Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

olfactory bulb has around 3000glomeruli(Meisami, 1979); a similararrange- ... Source: Vogt, Richard G. - Department of Biological Sciences, University of South Carolina...

285

A Role for Endoglin as a Suppressor of Malignancy during Mouse Skin Carcinogenesis  

Science Journals Connector (OSTI)

...Maria Villa-Morales 2 Javier Santos 2 Jose Fernandez-Piqueras...respectively, and Eva G. Santos for skilful technical assistance...Cano A, Perez-Moreno MA, Rodrigo I, et al. The transcription...537-43. 29 Villa-Morales M, Santos J, Fernandez-Piqueras J...

Eduardo Pérez-Gómez; María Villa-Morales; Javier Santos; José Fernández-Piqueras; Carlos Gamallo; Javier Dotor; Carmelo Bernabéu; and Miguel Quintanilla

2007-11-01T23:59:59.000Z

286

Progesterone receptors in the mouse mammary duct: distribution and developmental regulation  

Science Journals Connector (OSTI)

...expressing the 143Ala p53 temperature-sensitive mutant, we demonstrated...Sensitivity and Specificity Temperature Transfection Transplantation...Wild-Type p53 and a p53 Temperature-sensitive Mutant Suppress...Aiqing Lang, Tong Ge, Dantong Sun, Ming Tan, and Dihua Yu2...

GB Silberstein; K Van Horn; G Shyamala; and CW Daniel

1996-07-01T23:59:59.000Z

287

The effects of adult male mouse urine odor on evoked potentials in adult female mice  

E-Print Network [OSTI]

collected with EEG electrodes placed within a cup of physiological saline solution and SCI-LAB turned on Power spectrum of data collected with EEG electrodes placed within a cup of physiological saline solution and SCI-LAB turned off 56 10. Power... spectrum (test 1) of data collected with EEG electrodes tied together and placed within a cup of physiological saline solution 59 power spectrum (test 2) of data collected with EEG electrodes tied together and placed within a cup of physiological...

Brown, Troy Edwin

2012-06-07T23:59:59.000Z

288

Identification of Neuronal Enhancers of the Proopiomelanocortin Gene by Transgenic Mouse Analysis and Phylogenetic Footprinting  

Science Journals Connector (OSTI)

...1428 Buenos Aires, Argentina. Phone: 5411-47832871...Aires, Buenos Aires, Argentina 2 Vollum Institute 3...role in the control of energy homeostasis, and rare...fellowships from CONICET, Argentina. M.J.L. declares...leptin regulation of energy balance but not reproduction...

Flávio S. J. de Souza; Andrea M. Santangelo; Viviana Bumaschny; María Elena Avale; James L. Smart; Malcolm J. Low; Marcelo Rubinstein

2005-04-01T23:59:59.000Z

289

Metabolism of an Antineoplastic Methylhydrazine Derivative in a P815 Mouse Neoplasm  

Science Journals Connector (OSTI)

...be introduced into guanine because the hydrogen in the 2-position would be substituted...Adenine and guanine lost 4 and 5 hydrogen atoms, respectively, but each retained...J., and Chase, M. Nucleic Acid Economy in Bacteria Infected with Bacteriophage...

W. Kreis

1970-01-01T23:59:59.000Z

290

TRP channel gene expression in the mouse retina Jared C. Gilliam, Theodore G. Wensel  

E-Print Network [OSTI]

1 in the photoreceptor inner segment layer, for TRPM1 in the inner nuclear layer (INL), for TRPM3 in the INL, and for TRPML1 in the outer plexiform and nuclear layers. Strong immunofluorescence signal in cone outer segments was observed for TRPM7 and TRPP2. TRPC5 immunostaining was largely confined to INL

Wensel, Theodore G.

291

MouseLight: Bimanual Interactions on Di Using a Pen and a  

E-Print Network [OSTI]

Guimbretiere 1 Autodesk Research 210 King St. East, Toronto, ON, M5A 1J7 Canada {firstname.lastname}@autodesk

Keinan, Alon

292

Interleukin 7 (IL-7) selectively promotes mouse and human IL-17–producing ?? cells  

Science Journals Connector (OSTI)

...36 Komschlies KL ( 1994 ) Administration of recombinant human IL-7...Geiselhart LA ( 2001 ) IL-7 administration alters the CD4:CD8 ratio...11 ): 1255 – 1265 . 45 Bonneville M O’Brien RL Born WK...Quantitative PCR was performed with Power SYBR Green PCR Master Mix...

Marie-Laure Michel; Dick J. Pang; Syeda F. Y. Haque; Alexandre J. Potocnik; Daniel J. Pennington; Adrian C. Hayday

2012-01-01T23:59:59.000Z

293

Building a better mousetrap: Compressing mouse cursor activity for web analytics  

Science Journals Connector (OSTI)

Abstract Websites can learn what their users do on their pages to provide better content and services to those users. A website can easily find out where a user has been, but in order to find out what content is consumed and how it was consumed at a sub-page level, prior work has proposed client-side tracking to record cursor activity, which is useful for computing the relevance for search results or determining user attention on a page. While recording cursor interactions can be done without disturbing the user, the overhead of recording the cursor trail and transmitting this data over the network can be substantial. In our work, we investigate methods to compress cursor data, taking advantage of the fact that not every cursor coordinate has equal value to the website developer. We evaluate 5 lossless and 5 lossy compression algorithms over two datasets, reporting results about client-side performance, space savings, and how well a lossy algorithm can replicate the original cursor trail. The results show that different compression techniques may be suitable for different goals: LZW offers reasonable lossless compression, but lossy algorithms such as piecewise linear interpolation and distance-thresholding offer better client-side performance and bandwidth reduction.

Luis A. Leiva; Jeff Huang

2015-01-01T23:59:59.000Z

294

Reflectance and Fluorescence Confocal Microscope for Imaging of the Mouse Colon  

E-Print Network [OSTI]

and fluorescence microscope. 20 3.3 Image Formation To form a 2D image using a point-scanning confocal microscope, the beam must be scanned in two dimensions using scanning mirrors. The PMT records a voltage signal from each point which is assigned... and fluorescence microscope. 20 3.3 Image Formation To form a 2D image using a point-scanning confocal microscope, the beam must be scanned in two dimensions using scanning mirrors. The PMT records a voltage signal from each point which is assigned...

Saldua, Meagan Alyssa

2012-02-14T23:59:59.000Z

295

RESPONSE OF THE SWISS ALBINO MOUSE TO THE VIRUS OF NEWCASTLE DISEASE  

Science Journals Connector (OSTI)

...NEWCASTLE DISEASE Reginald L. Reagan A. L. Brueckner Maryland State Board of Agriculture...DISEASE REGINALD L. REAGAN AND A. L. BRUECKNER Maryland State Board of Agriculture...4-6 453 REGINALD L. REAGAN AND A. L. BRUECKNER used for the initial inoculum. Three-hundredths...

Reginald L. Reagan; A. L. Brueckner

1951-04-01T23:59:59.000Z

296

EFFECT OF WEST NILE VIRUS IN THE SWISS ALBINO MOUSE AND THE CAVE BAT  

Science Journals Connector (OSTI)

...M. Schenck M. P. Harmon A. L. Brueckner Live Stock Sanitary Service Laboratory...SCHENCK, M. P. HARMON, AND A. L. BRUECKNER Live Stock Sanitary Service Laboratory...nervous 79 REAGAN, SCHENCK, HARMON, AND BRUECKNER system involvement while the unized mice...

Reginald L. Reagan; D. M. Schenck; M. P. Harmon; A. L. Brueckner

1952-07-01T23:59:59.000Z

297

Picropodophyllin inhibits tumor growth of human nasopharyngeal carcinoma in a mouse model  

SciTech Connect (OSTI)

Highlights: •We identified that PPP inhibits IGF-1R/Akt pathway in NPC cells. •PPP dose-dependently inhibits NPC cell proliferation in vitro. •PPP suppresses tumor growth of NPC in nude mice. •PPP have little effect on microtubule assembly. -- Abstract: Insulin-like growth factor-1 receptor (IGF-1R) is a cell membrane receptor with tyrosine kinase activity and plays important roles in cell transformation, tumor growth, tumor invasion, and metastasis. Picropodophyllin (PPP) is a selective IGF-1R inhibitor and shows promising antitumor effects for several human cancers. However, its antitumor effects in nasopharyngeal carcinoma (NPC) remain unclear. The purpose of this study is to investigate the antitumor activity of PPP in NPC using in vitro cell culture and in vivo animal model. We found that PPP dose-dependently decreased the IGF-induced phosphorylation and activity of IGF-1R and consequently reduced the phosphorylation of Akt, one downstream target of IGF-1R. In addition, PPP inhibited NPC cell proliferation in vitro. The half maximal inhibitory concentration (IC50) of PPP for NPC cell line CNE-2 was ?1 ?M at 24 h after treatment and ?0.5 ?M at 48 h after treatment, respectively. Moreover, administration of PPP by intraperitoneal injection significantly suppressed the tumor growth of xenografted NPC in nude mice. Taken together, these results suggest targeting IGF-1R by PPP may represent a new strategy for treatment of NPCs with positive IGF-1R expression.

Yin, Shu-Cheng [Department of Otolaryngology – Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060 (China) [Department of Otolaryngology – Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060 (China); Department of Otolaryngology – Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071 (China); Guo, Wei [Department of Otolaryngology – Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071 (China)] [Department of Otolaryngology – Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071 (China); Tao, Ze-Zhang, E-mail: zezhangtao@gmail.com [Department of Otolaryngology – Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060 (China)] [Department of Otolaryngology – Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060 (China)

2013-09-13T23:59:59.000Z

298

E-Print Network 3.0 - ames dwarf mouse Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

object is a brown dwarf. However, the use of this so-called lithium Source: Stancil, Phillip C. - Department of Physics and Astronomy, University of Georgia Collection: Physics...

299

Mouse model implicates GNB3 duplication in a childhood obesity syndrome  

Science Journals Connector (OSTI)

...Mulle Shikha Dharamrup Madhuri R. Hegde Katherine H. Kim Brad Angle Alison Colley Amy E...Hum Genet 87 ( 5 ): 661 – 666 . 14 Wheeler E ( 2013 ) Genome-wide SNP and CNV analysis identifies common...Hum Genet 13 ( 3 ): 361 – 369 . 36 Wheeler PG Weaver DD Palmer CG ( 1995 ) Familial...

Ian S. Goldlust; Karen E. Hermetz; Lisa M. Catalano; Richard T. Barfield; Rebecca Cozad; Grace Wynn; Alev Cagla Ozdemir; Karen N. Conneely; Jennifer G. Mulle; Shikha Dharamrup; Madhuri R. Hegde; Katherine H. Kim; Brad Angle; Alison Colley; Amy E. Webb; Erik C. Thorland; Jay W. Ellison; Jill A. Rosenfeld; Blake C. Ballif; Lisa G. Shaffer; Laurie A. Demmer; Unique Rare Chromosome Disorder Support Group; M. Katharine Rudd

2013-01-01T23:59:59.000Z

300

Requirement of Sur2 for Efficient Replication of Mouse Adenovirus Type 1  

Science Journals Connector (OSTI)

...Type 1 Lei Fang 1 Jennitte L. Stevens 2 Arnold J. Berk 2 Katherine R. Spindler 3 Corresponding author. Mailing address: Department...Acad. Sci. USA 99: 12747-12752. 3 Avvakumov, N., R. Wheeler, J. C. DHalluin, and J. S. Mymryk. 2002. Comparative...

Lei Fang; Jennitte L. Stevens; Arnold J. Berk; Katherine R. Spindler

2004-12-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

The relationship among tumor architecture, pharmacokinetics, pharmacodynamics, and efficacy of bortezomib in mouse xenograft models  

Science Journals Connector (OSTI)

...at 37C, 5% CO2 atmosphere, 97% relative humidity...done on a 7T (300 MHz) 210-mm horizontal...spectral width = 100 kHz. Each image took...similar area under the plasma concentration-time...relationship between plasma and tumor pharmacokinetics...tumor perfusion than plasma drug exposures...

Mark J. Williamson; Matthew D. Silva; Jennifer Terkelsen; Robbie Robertson; Li Yu; Cindy Xia; Panos Hatsis; Bret Bannerman; Trisha Babcock; Yueying Cao; and Erik Kupperman

2009-12-01T23:59:59.000Z

302

The relationship among tumor architecture, pharmacokinetics, pharmacodynamics, and efficacy of bortezomib in mouse xenograft models  

Science Journals Connector (OSTI)

...cultured at 37C, 5% CO2 atmosphere, 97% relative humidity...was done on a 7T (300 MHz) 210-mm horizontal...spectral width = 100 kHz. Each image took 5...similar area under the plasma concentration-time...relationship between plasma and tumor pharmacokinetics...tumor perfusion than plasma drug exposures (57...

Mark J. Williamson; Matthew D. Silva; Jennifer Terkelsen; Robbie Robertson; Li Yu; Cindy Xia; Panos Hatsis; Bret Bannerman; Trisha Babcock; Yueying Cao; and Erik Kupperman

2009-12-01T23:59:59.000Z

303

An extensive list of genes that produce alternative transcripts in the mouse  

Science Journals Connector (OSTI)

......symbol chromosome J numbers symbol a 2 J:12911 ASIP J:18750 J:4186 Abl 2 J:40703 ABL1 Acadl 1 J:28013 ACADL Acads 5 J:12776 ACADS Accn1 UN J:44171 ACCN1 Adss2 UN J:16814 NULL Ags X J:30307 GLA J:33774 Akap UN J:39210 AKAP84-PEN Axin 17......

ME Mangan; KS Frazer

1999-02-01T23:59:59.000Z

304

Intravital imaging of amyloid plaques in a transgenic mouse model using optical-resolution  

E-Print Network [OSTI]

; revised November 10, 2009; accepted November 10, 2009; posted November 12, 2009 (Doc. ID 114001 to play a major role in the pathogen- esis of Alzheimer's disease (AD), but the underlying mechanisms

Wang, Lihong

305

An Extract of Phyllanthus amarus Protects Mouse Chromosomes and Intestine from Radiation Induced Damages  

Science Journals Connector (OSTI)

......using Graphpad Instat 3 Software (San Diego, USA...Effect of P.amarus on radiation induced changes in the...Mr. M. Muthuvel, Radiation safety Officer, Amala Cancer...Withers, H. R. (1999) Radiation biology and treatment......

Kuzhuvelil Bhaskaran Nair Harikumar; Ramadasan Kuttan

2007-11-01T23:59:59.000Z

306

Computer simulations reveal complex distribution of haemodynamic forces in a mouse retina model of angiogenesis  

Science Journals Connector (OSTI)

...UK-Israel Initiative BIRAX; EPSRC grants 2020 Science ( http://www.2020science...www.cresta-project.eu/ , grant no. 287703) and MAPPER ( http://www.mapper-project.eu/ , grant no. 261507). C.A.F. is supported...

2014-01-01T23:59:59.000Z

307

Production and Characterization of Mouse Monoclonal Antibodies to Human Bladder Tumor-associated Antigens  

Science Journals Connector (OSTI)

...This report describes the production of four murine McAbs against...harvested with the aid of a rubber policeman and washed three...enough to screen for antibody production (about 3 weeks). Screening...as feeder cells. Antibody Production and Purification. As a source...

Deborah A. Young; George R. Prout, Jr.; and Chi-Wei Lin

1985-09-01T23:59:59.000Z

308

Mechanism of H-ras Oncogene Activation in Mouse Squamous Carcinoma Induced by an Alkylating Agent  

Science Journals Connector (OSTI)

...tumors, DMBA activated H-ras in at least 75...carcinoma model (14), H-ras was activated in...contains an ethylcarbonyl bridge between the N-l and N...the l^V*-etheno- bridge-adenine adduct formed...the middle adenine of the H-ras 61st codon. ACKNOWLEDGMENTS...

Anne E. Hochwalt; Jerome J. Solomon; and Seymour J. Garte

1988-02-01T23:59:59.000Z

309

The use of mouse models to elucidate the genetic and environmental components of neural tube defects  

E-Print Network [OSTI]

in the hindgut endoderm in CT embryos developing spinal NTDs was delayed, the intensity of RARy mRNA was lower in the 14 neuroepithelium and mesoderm of the PNP region in affected CT embryos, and that the decrease in RARy mRNA levels was directly correlated... region (Brook et aL, 1991). Copp and coworkers (1988a) observed that proliferation in the gut endoderm and notochord cells of the PNP region was reduced in embryos developing spinal NTDs compared with normally developing controls. However, no change...

Gefrides, Lisa Anne

2012-06-07T23:59:59.000Z

310

Hormonal effects on the development of the mouse ovary in vitro  

E-Print Network [OSTI]

, and cultures with TGF-S, EGF, GH, PRL, and FSH stimulate the formation of collagen deposits in the ovaries. Also, the latter combination appeared to disrupt the deposition of the basal lamina around the fol 1 i cl es. Several treatments, including EGF alone... of the dorsal surface of the coelomic epithelium on fetal day 9. The primordial germ cells begin migrating into this area from the yolk sac endoderm at about fetal day 10 , and have essentially completed their migration by fetal day 12. Ovaries and testes...

Mitchell, Philip Allen

2012-06-07T23:59:59.000Z

311

Retinal Degeneration and Ionizing Radiation Hypersensitivity in a Mouse Model for Cockayne Syndrome  

Science Journals Connector (OSTI)

...sensitive to a single dose of ionizing radiation at doses up to 10 Gy. In marked...least within the age range investigated, the...photoreceptor cells for ionizing radiation. This finding underscores...experiment (cumulative dose, 35 kJ/m2). The...

Theo G. M. F. Gorgels; Ingrid van der Pluijm; Renata M. C. Brandt; George A. Garinis; Harry van Steeg; Gerard van den Aardweg; Gerard H. Jansen; Jan M. Ruijter; Arthur A. B. Bergen; Dirk van Norren; Jan H. J. Hoeijmakers; Gijsbertus T. J. van der Horst

2006-12-04T23:59:59.000Z

312

Identification of Neuronal Enhancers of the Proopiomelanocortin Gene by Transgenic Mouse Analysis and Phylogenetic Footprinting  

Science Journals Connector (OSTI)

...overnight at 4C, and sectioned (50 mum) with Vibratome 1000 (Ted Pella, Redding, Calif.). Brain slices were treated with...of transcription. Mol. Cell. Biol. 17: 5952-5959. 44 Poulin, G., B. Turgeon, and J. Drouin. 1997. NeuroD1/beta2...

Flávio S. J. de Souza; Andrea M. Santangelo; Viviana Bumaschny; María Elena Avale; James L. Smart; Malcolm J. Low; Marcelo Rubinstein

2005-04-01T23:59:59.000Z

313

The Combined Effects of MRI and X-rays on ICR Mouse Embryos during Organogenesis  

Science Journals Connector (OSTI)

......average fetal response within a litter was calculated. For statistical tests, we used non-parametric methods; a Kruskal-Wallis test for a comparison among groups or a Wilcoxon test for comparisons between two groups16) . RESULTS Intrauterine......

Yeunhwa Gu; Takeo Hasegawa; Youichi Yamamoto; Michiaki Kai; Tomoko Kusama

2001-09-01T23:59:59.000Z

314

Response of ?-Irradiated Mouse Salivary Gland Cells to a Proliferative Stimulus  

Science Journals Connector (OSTI)

...Takehito Sasaki Takeo Nogami Department of Radiology, Tokyo Medical and Dental University, School of Dentistry, Bunkyo-ku...Takehito Sasaki and Takeo Nogami Department of Radiology, Tokyo Medical and Dental University, School of Dentistry, Bunkyo-ku...

Takehito Sasaki and Takeo Nogami

1973-07-01T23:59:59.000Z

315

Shedding and Intracage Transmission of Sin Nombre Hantavirus in the Deer Mouse (Peromyscus maniculatus) Model  

Science Journals Connector (OSTI)

...respectively. The photoperiod (hours of light:hours of dark) during the study was...membrane was cut lengthwise with a paper shredder into 1.6-mm strips, and each strip...viral RNA can be found in the particulate fraction of breast milk, but neutralizing antibodies...

Jason Botten; Katy Mirowsky; Chunyan Ye; Keith Gottlieb; Melissa Saavedra; Liana Ponce; Brian Hjelle

2002-08-01T23:59:59.000Z

316

Induction of Retrovirus Gene Expression in Mouse Cells by Some Chemical Mutagens  

Science Journals Connector (OSTI)

...Myer, and Ralph J. Rascati3 Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 3 7830 ABSTRACT Cell cultures derived...physical stimuli (reviewed in Ref. 39). Previous workers have explored the mechanism(s) of induction...

Raymond W. Tennant; James A. Otten; Fred E. Myer; and Ralph J. Rascati

1982-08-01T23:59:59.000Z

317

The Effect of Transplanted and Spontaneous Mouse Mammary Gland Carcinomas on Lymph Nodes  

Science Journals Connector (OSTI)

...as reported by other workers, but also, when the...experiments was furnished by the Oak Ridge National Laboratory...been ad vanced by other workers (9). That implanted...as reported by other workers, but also, when the...

S. Albert; Ralph M. Johnson; Hermann Pinkus; and Lydia Horn

1954-11-01T23:59:59.000Z

318

DNA Transfection of Ecotropic Murine Leukemia Viruses in Mouse Cell Cultures  

Science Journals Connector (OSTI)

...and Toxicology Program, Biology Division, Oak Ridge National Laboratory, Post Office Box Y, Oak Ridge, Tenn. 37830. DNA's were isolated from...preparations has since been established by workers in several laboratories (2, 6, 7, 10...

Ih-Chang Hsu and Wen K. Yang

1977-06-01T23:59:59.000Z

319

Development, characterization and transcriptional profiling of a mouse model of fatal infectious diarrhea and colitis  

E-Print Network [OSTI]

Citrobacter rodentium is a naturally occurring murine bacterial pathogen which is used to model human diarrheagenic E. coli (EPEC and EHEC) infections in mice. C. rodentium causes colonic hyperplasia and a variable degree ...

Borenshtein, Diana

2007-01-01T23:59:59.000Z

320

Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptor  

E-Print Network [OSTI]

Lafayette, IN, and approved May 19, 2011 (received for review March 17, 2011) Coronaviruses have evolved

Li, Fang

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Analysis of pluripotent mouse stem cell proteomes : insights into post- transcriptional regulation of pluripotency and differentiation  

E-Print Network [OSTI]

Protein KIAA1967 ho 589.07 Vim Vimentin 273.11 Etfa Electron2610301G19RikIPI00123624 Vim IPI00227299 Etfa IPI00116753375.33 substrate Prkar2b Cfl2 Vim IPI00224570 IPI00266188

O'Brien, Robert Norman

2010-01-01T23:59:59.000Z

322

Novel Endogenous Type D Retroviral Particles Expressed at High Levels in a SCID Mouse Thymic Lymphoma  

Science Journals Connector (OSTI)

...Novel Endogenous Type D Retroviral Particles Expressed at High Levels in a SCID...University of Ballarat, St. John of God Hospital, Ballarat, Victoria 3350...activity. Electron microscopy revealed particles with type D retroviral morphology...

Sika Ristevski; Damian F. J. Purcell; John Marshall; Daniella Campagna; Sara Nouri; Simon P. Fenton; Dale A. McPhee; George Kannourakis

1999-06-01T23:59:59.000Z

323

Interaction between purine and benzodiazepine: Inosine reverses diazepam-induced stimulation of mouse exploratory behavior  

Science Journals Connector (OSTI)

...127, 267 (1978)]. The arti-ficial EOD signals, which were not phase-locked...anatomical connections (efferent copy of the EOD) exist between the pacemaker and elec-trosensory...these types of free-running artificial EOD signals are sufficient to drive behaviors...

JN Crawley; PJ Marangos; SM Paul; P Skolnick; FK Goodwin

1981-02-13T23:59:59.000Z

324

Microsoft Word - FINAL 2010_SPFPA_CBA With MOUs.doc  

National Nuclear Security Administration (NNSA)

Violations shall be cause for immediate discharge of any violators. There shall be no lockout of employees by the Company during the life of this Agreement. ARTICLE 9. GENERAL...

325

A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland  

E-Print Network [OSTI]

% glutaraldehyde in 0.1 mol/l sodium cacodylate/HCl buffer of pH 7.2–7.4 at 4°C for 48 h. After washing with distilled water (dH2O) for 20 min, the samples underwent secondary fixation in 1% osmium tetroxide in dH2O for 45 min at room temperature. Samples were...

Gordon, Katrina E; Binas, Bert; Chapman, Rachel S; Kurian, Kathreena M; Clarkson, Richard W E; Clark, A John; Birgitte Lane, E; Watson, Christine J

2000-03-07T23:59:59.000Z

326

Hybrid feature selection and SVM-based classification for mouse skin  

E-Print Network [OSTI]

wavelengths," Lasers Surg. Med. 19, 63­74 (1996). 4. W. C. Lin, S. A. Toms, M. Johnson, E. D. Jansen, and A. Mahadevan-Jansen, "In vivo brain tumor demarcation using optical spectroscopy," Photochem Photobiol. 73, 396­402 (2001). 5. W. C. Lin, S. A. Toms, M. Motamedi, E. D. Jansen, and A. Mahadevan-Jansen, "Brain tumor

Guermeur, Yann

327

Investigations into arsenate-induced neural tube defects in a mouse model  

E-Print Network [OSTI]

. Of the many environmental agents considered to potentially contribute to NTD risk, arsenic is one that is surrounded in controversy. We have developed a model system utilizing maternal intraperitoneal (I.P.) exposure on E7.5 and E8.5 to As 9.6 mg/kg (as sodium...

Hill, Denise Suzanne

2009-05-15T23:59:59.000Z

328

In vivo super-penetration microscopy for noninvasive imaging of mouse brain  

Science Journals Connector (OSTI)

We developed a super-penetration microscope that effectively compensates the wavefront distortion encountered in scattering biological systems. We report high-resolution in vivo...

Cui, Meng

329

Exploring diazepam’s effect on hemodynamic responses of mouse brain tissue by optical spectroscopic imaging  

Science Journals Connector (OSTI)

In this study, a simple duel-optical spectroscopic imaging apparatus capable of simultaneously determining relative changes in brain oxy-and deoxy-hemoglobin concentrations was used...

Abookasis, David; Shochat, Ariel; Nesher, Elimelech; Pinhasov, Albert

2014-01-01T23:59:59.000Z

330

Cell type and differentiation dependent heterogeneity in retinoblastoma protein expression in SCID mouse fetuses  

Science Journals Connector (OSTI)

...genetics Fetus chemistry Fluorescent Antibody Technique Genes...blotting (1 1, 35), has led to the conclu-sion...Superficial cells Hair bulbs Coluninal epithelia of...the nuclei of a hair bulb of a vibrissa visualized...was used as a second fluorescent layer for the sections...

L Szekely; WQ Jiang; F Bulic-Jakus; A Rosen; N Ringertz; G Klein; and KG Wiman

1992-03-01T23:59:59.000Z

331

Antiangiogenic Treatment Enhances Photodynamic Therapy Responsiveness in a Mouse Mammary Carcinoma  

Science Journals Connector (OSTI)

...generated by a parallel series of red Mylar-filtered 30 W fluorescent bulbs and delivered at a dose rate of 0.35 mW/cm2. In...histologically documented tumor necrosis originally led to suggestions that hypoxia is a major regulator of...

Angela Ferrario; Karl F. von Tiehl; Natalie Rucker; Margaret A. Schwarz; Parkash S. Gill; and Charles J. Gomer

2000-08-01T23:59:59.000Z

332

Mucosal Immunization with a Candidate Universal Influenza Vaccine Reduces Virus Transmission in a Mouse Model  

Science Journals Connector (OSTI)

...microisolator lids (191 by 292 by 127 mm) in a Smart Bio-Pak rack (model SB4100; Allentown...Dividers were held in place with a wire grid (Ancare) providing water bottles and...cross-protective vaccines may provide benefit to both vaccinees and the nonvaccinated...

Graeme E. Price; Chia-Yun Lo; Julia A. Misplon; Suzanne L. Epstein

2014-03-12T23:59:59.000Z

333

Transgenic Nude Mouse with Ubiquitous Green Fluorescent Protein Expression as a Host for Human Tumors  

Science Journals Connector (OSTI)

...detected with a simple blue-light-emitting diode flashlight with a central...detected with a simple blue-light-emitting diode flashlight with a central...detected with a simple blue-light-emitting diode flashlight with a central...

Meng Yang; Jose Reynoso; Ping Jiang; Lingna Li; Abdool R. Moossa; Robert M. Hoffman

2004-12-01T23:59:59.000Z

334

Ceramide Generation in Response to Photodynamic Treatment of L5178Y Mouse Lymphoma Cells  

Science Journals Connector (OSTI)

...PDT, photodynamic therapy; MB, methylene blue; TB, toluidine blue; IAA, indole-3-acetic acid; LED, light-emitting diode; MOI, 3-methylene-2-oxindole; HPLC, high-performance liquid chromatography. Fig. 1. Pathways involved...

Duska Separovic; Jin He; Nancy L. Oleinick

1997-05-01T23:59:59.000Z

335

The naïve airway hyperresponsiveness of the A/J mouse is Kit-mediated  

Science Journals Connector (OSTI)

...in certain antigen-induced models of pulmonary inflammation, and dendritic cells...Immunol 107 : 963 – 970 . 11 Flood-Page P International Mepolizumab Study Group ( 2007...JB-4 glycol methacrylate polymer with a commercially available...digital camera and HC Image software 1.1.3.1 (Hama-matsu...

Emily Cozzi; Kate G. Ackerman; Anders Lundequist; Jeffrey M. Drazen; Joshua A. Boyce; David R. Beier

2011-01-01T23:59:59.000Z

336

The Activity of 6-Selenopurine and Related Compounds against Some Experimental Mouse Tumors  

Science Journals Connector (OSTI)

...Antileukemic Action of 6-Mercaptopurine in Mice. Ann. N.Y. Acad. Sc., 60:251-66, 1954. 5. Handbook of Chemistry and Physics, 35th ed., p. 290. Cleveland: Chemical Rubber Publishing Co., 1953-54. 6. HEIDELBERGER,C...

Henry G. Mautner and Julian J. Jaffe

1958-04-01T23:59:59.000Z

337

Induction of the Polyamine-biosynthetic Enzymes in Mouse Epidermis by Tumor-promoting Agents  

Science Journals Connector (OSTI)

...activity reached a peak (230-fold greater...5 hr after croton oil or TPA treatment and...activity reached a peak (230-fold greater...5 hr after croton oil or TPA treatment and...activity to croton oil differed from that...in 2 respects: the peak of activity following...

T. G. O'Brien; R. C. Simsiman; and R. K. Boutwell

1975-07-01T23:59:59.000Z

338

The Induction of Ornithine Decarboxylase as an Early, Possibly Obligatory, Event in Mouse Skin Carcinogenesis  

Science Journals Connector (OSTI)

...increased after croton oil treatment, reaching a peak (approximately 6-fold...observed after croton oil application, except the peak value of activity...general, however, the peak of activity occurred...with TPA or croton oil. This difference...

T. G. O'Brien

1976-07-01T23:59:59.000Z

339

Characterization of Urinary Iron Loss in the fsn (flaky skin) Anemia Mouse Mutant  

E-Print Network [OSTI]

Iron overloading is a serious medical problem for blood transfusion-dependent diseases such as sickle cell disease, ?–Thalassemia Major, and Myelodysplastic syndromes which require chronic blood transfusions to treat their related...

Kress, Robert Lee

2014-08-31T23:59:59.000Z

340

Utilization of the E?-Myc Mouse to Model Heterogeneity of Therapeutic Response  

Science Journals Connector (OSTI)

...model, and establishes a framework to test novel targeted therapies or combination...two clusters using the Wilcoxon rank sum test and Bonferonni correction (q value 0...Significance determined by the log-rank test. Moving beyond descriptive analyses...

Rachel E. Rempel; Xiaolei Jiang; Paul Fullerton; Tuan Zea Tan; Jieru Ye; Jieying Amelia Lau; Seiichi Mori; Jen-Tsan Chi; Joseph R. Nevins; Daphne R. Friedman

2014-12-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

The role of retinoic acid in germ cell development in embryonic mouse gonads  

E-Print Network [OSTI]

Germ cells are the only cell type to undergo meiosis, a specialized cell division process necessary for the formation of haploid gametes. Timing of this process is sex-specific. Ovarian germ cells initiate meiosis during ...

Koubová, Jana C

2007-01-01T23:59:59.000Z

342

Induction of androgen receptor formation by epithelium-mesenchyme interaction in embryonic mouse mammary gland  

Science Journals Connector (OSTI)

...The eptelu in the' uppe center'" aglan wit Loalizadheiongi of [~epidsermi tp) oethtte...D.ih a o a explant 3asi associato wit salivar eihiu Inaotne thr of such combination...London) New Biol. 232, 5-7. 20. Price, D. & Ortiz, E. (1965) in Organogenesis...

B Heuberger; I Fitzka; G Wasner; K Kratochwil

1982-01-01T23:59:59.000Z

343

Mouse Dipeptidyl Peptidase 4 Is Not a Functional Receptor for Middle East Respiratory Syndrome Coronavirus Infection  

Science Journals Connector (OSTI)

...Virol. 88 :1834-1838. doi: 10.1128/JVI.02935-13 . 6. de Wit, E , J Prescott, L Baseler, T Bushmaker, T Thomas, MG Lackemeyer, C Martellaro, S Milne-Price, E Haddock, BL Haagmans, H Feldmann, and VJ Munster. 2013. The...

Adam S. Cockrell; Kayla M. Peck; Boyd L. Yount; Sudhakar S. Agnihothram; Trevor Scobey; Nicole R. Curnes; Ralph S. Baric; Mark T. Heise

2014-02-26T23:59:59.000Z

344

Amyloid protein SAA is an apoprotein of mouse plasma high density lipoprotein  

Science Journals Connector (OSTI)

...less cumbersome and more rapid method of thermal denaturation, which has been used to...6 M urea buffer were subjected to this thermal denaturation. Antigen solutions in the...migration (anode at bot- tom); stain, Sudan black B. plasma is illustrated in Fig...

E P Benditt; N Eriksen; R H Hanson

1979-01-01T23:59:59.000Z

345

Cribriform Degeneration (cri): A New Recessive Neurological Mutation in the Mouse  

Science Journals Connector (OSTI)

...were uncolored in frozen or wax sections stained for lipids (Sudan dyes), or for carbohydrate polymers (periodic acid-Schiff...nonrepeated DNA with total nonradioactive DNA, followed by assay of thermal stability (10). The albumin immunological distances were...

Margaret C. Green; Richard L. Sidman; Omar H. Pivetta

1972-05-19T23:59:59.000Z

346

The tumor cells were derived from primary mouse medulloblastomas, which are cerebel-  

E-Print Network [OSTI]

the active compound classes, which suggests that common off- target effects are unlikely, and inhibition of the antiproliferative effect of the D2/3 dopa- mine receptor agonist bromocriptine by coad- ministration of the D2 with Parkinson disease10. Finally, the possibility that neu- rotransmitters are involved in cancer stem cell self

Doyle, Patrick S.

347

Identification of a Mouse Thiamine Transporter Gene as a Direct Transcriptional Target for p53*  

E-Print Network [OSTI]

- mine transporter activities were found in cells overex- pressing mTHTR-1 and under conditions of DNA damage or p53 activation. Our findings indicate that p53 may be involved in maintaining thiamine frequent events in human cancers. p53 has been shown to inhibit cell cycle progression, promote apoptosis

Lin, Chi-Hung

348

Growth Inhibition and Augmentation of Mouse Myeloid Leukemic Cell Line Differentiation by Interleukin 1  

Science Journals Connector (OSTI)

...PCS at a density of 1 x IO6 cells/ml were cultured with or without LPS (10 /ig/mi) for 3 days. The culture supernal inis were dialyzed against RPMI 1640, and the IL 1 activity in the supernatants was determined. RESULTS Effect of IL 1 on Growth...

Kikuo Onozaki; Takuya Tamatani; Tatsuichiro Hashimoto; Kouji Matsushima

1987-05-01T23:59:59.000Z

349

Odorant Receptor Expression Defines Functional Units in the Mouse Olfactory System  

E-Print Network [OSTI]

) and influ- ence the guidance of OSN axons to synaptic targets in the olfactory bulb (OB). OSNs expressing of single neurons from gene- targeted mice in which the green fluorescent protein is coex- pressed and arrangement of glomerular functional units. Key words: olfaction; olfactory system; olfactory bulb; glo

Bozza, Thomas

350

False-positive apoptosis signal in mouse kidney and liver detected with TUNEL assay  

Science Journals Connector (OSTI)

Apoptosis is a physiological, programmed process for the elimination of cells from living organisms. Currently, one of the most frequently used methods to detect apoptosis is TUNEL assay. It has provided valua...

K. J. Pulkkanen; M. O. Laukkanen; J. Naarala; S. Yla-Herttuala

2000-10-01T23:59:59.000Z

351

Energy Balance Modulates Mouse Skin Tumor Promotion through Altered IGF-1R and EGFR Crosstalk  

Science Journals Connector (OSTI)

...and growth factor starved for...Results Dietary energy balance effects...malignant conversion (see also...that dietary energy balance modulated...steady-state growth factor signaling...Berger NA.Energy balance, host-related factors, and cancer...

Tricia Moore; Linda Beltran; Steve Carbajal; Stephen D. Hursting; and John DiGiovanni

2012-10-01T23:59:59.000Z

352

Novel Vitamin E Analogue Decreases Syngeneic Mouse Mammary Tumor Burden and Reduces Lung Metastasis  

Science Journals Connector (OSTI)

...Sun Vernon Knight Brian E. Gilbert Claudio Conti Bob G. Sanders...of Molecular Virology and Microbiology B. E. G., Baylor College...17 Koshkina, N. V., Gilbert, B. E., Waldrep, J...Giovanella, B. C., and Gilbert, B. E. Anticancer effect...

Karla A. Lawson; Kristen Anderson; Marla Menchaca; Jeffrey Atkinson; LuZhe Sun; Vernon Knight; Brian E. Gilbert; Claudio Conti; Bob G. Sanders; and Kimberly Kline

2003-05-01T23:59:59.000Z

353

Distinct roles of the methylcytosine oxidases Tet1 and Tet2 in mouse embryonic stem cells  

Science Journals Connector (OSTI)

...913-0.132 0.235 0.454 0.270 0.435 Araf 1890.491 1696.876 2084.105 1.228 0.297 0.001 0.004 0.000 0.000 Cfp 165.666 165.687 165.645 1.000 0.000 0.811 0.978 0.832 0.898 Timp1 1334.914 1091.977 1577.850 1.445...

Yun Huang; Lukas Chavez; Xing Chang; Xue Wang; William A. Pastor; Jinsuk Kang; Jorge A. Zepeda-Martínez; Utz J. Pape; Steven E. Jacobsen; Bjoern Peters; Anjana Rao

2014-01-01T23:59:59.000Z

354

Sonodynamic therapy inhibits angiogenesis and tumor growth in a xenograft mouse model  

E-Print Network [OSTI]

, Limin Jia b , Wenwu Cao a,c, a Laboratory of Sono- and Photo-theranostic Technologies, Harbin Institute

Cao, Wenwu

355

Effect of Aromatase Inhibitors on Growth of Mammary Tumors in a Nude Mouse Model  

Science Journals Connector (OSTI)

...pulverized in liquid nitrogen and then homogenized in ice-cold MTG buffer containing 0.01 M Tris-HCI (pH 7.4), 0.0015...4-hydroxy-4-androstene-3,17-dione. on estrogen-de pendent processes in reproduction and breast cancer. Endocrinology, 100: 1684...

Wei Yue; Jiping Wang; Alexei Savinov; and Angela Brodie

1995-07-15T23:59:59.000Z

356

Human and Mouse VEGFA-Amplified Hepatocellular Carcinomas Are Highly Sensitive to Sorafenib Treatment  

Science Journals Connector (OSTI)

...Basel). Required cohort size was computed by power analysis to yield a power of at least 90% with an alpha value of 0.05...2013;36:254-7. 18. Siegel AB , Cohen EI, Ocean A, Lehrer D, Goldenberg A, Knox JJ, et alPhase...

Elad Horwitz; Ilan Stein; Mariacarla Andreozzi; Julia Nemeth; Avivit Shoham; Orit Pappo; Nora Schweitzer; Luigi Tornillo; Naama Kanarek; Luca Quagliata; Farid Zreik; Rinnat M. Porat; Rutie Finkelstein; Hendrik Reuter; Ronald Koschny; Tom Ganten; Carolin Mogler; Oren Shibolet; Jochen Hess; Kai Breuhahn; Myriam Grunewald; Peter Schirmacher; Arndt Vogel; Luigi Terracciano; Peter Angel; Yinon Ben-Neriah; and Eli Pikarsky

2014-06-01T23:59:59.000Z

357

Geometric representation of neuroanatomical data observed in mouse brain at cellular and gross levels  

E-Print Network [OSTI]

This dissertation studies two problems related to geometric representation of neuroanatomical data: (i) spatial representation and organization of individual neurons, and (ii) reconstruction of three-dimensional neuroanatomical regions from sparse...

Koh, Wonryull

2009-05-15T23:59:59.000Z

358

Early tissue patterning recreated by mouse embryonic fibroblasts in a three-dimensional environment  

E-Print Network [OSTI]

Cellular self-organization studies have been mainly focused on models such as Volvox, the slime mold Dictyostelium discoideum, and animal (metazoan) embryos. Moreover, animal tissues undergoing regeneration also exhibit ...

Quintana, Lluis

359

Mouse and joystick performance comparison for moving and stationary index tracking displays  

E-Print Network [OSTI]

for text selection CRT", ~E* ', 1978, 0 1. 21 8 . 8, 601 617. Department of Defense, Defense 87, September/October 1987, 32. Huchingson, R. D. New Horizons for Human Factors in Desi n, McGraw-Hill, New York, 1981, 117, 173-184. Jagacinski, Richard J... Wiley and Sons, New York, 1983, 112-121. McDonald, A. C. , and Lowe H. Feedback and Control S stems, Prentice-Hall Co. , Reston Virginia, 1981, 4-8. Mehr, M. "Two-axis manual positioning and tracking controls", A lied-Er onomics, 1973, Vol. 4, No. 3...

Frick, Douglas Jon

2012-06-07T23:59:59.000Z

360

Anti-VEGF Antibodies Mitigate the Development of Radiation Necrosis in Mouse Brain  

Science Journals Connector (OSTI)

...images. The authors thank Drs. Dinesh Thotala and Carlos Perez-Torres...1257-63. 17. Gonzalez J , Kumar AJ, Conrad CA Levin VA.Effect...Levin VA , Bidaut L, Hou P, Kumar AJ, Wefel JS, Bekele BN...31. de Groot JF , Fuller G, Kumar AJ, Piao Y, Eterovic K, Ji...

Xiaoyu Jiang; John A. Engelbach; Liya Yuan; Jeremy Cates; Feng Gao; Robert E. Drzymala; Dennis E. Hallahan; Keith M. Rich; Robert E. Schmidt; Joseph J.H. Ackerman; and Joel R. Garbow

2014-05-15T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

Exposure of mouse embryonic pancreas to metformin enhances the number of pancreatic progenitors  

Science Journals Connector (OSTI)

Developing beta cells are vulnerable to nutrient environmental signals. Early developmental processes that alter the number of pancreatic progenitors can determine the number of beta cells present at birth. Me...

Brigid Gregg; Lynda Elghazi; Emilyn U. Alejandro; Michelle R. Smith…

2014-12-01T23:59:59.000Z

362

Role of Reactive Oxygen Metabolites in Crocidolite Asbestos Toxicity to Mouse Macrophages  

Science Journals Connector (OSTI)

...fibers may catalyze the production of hydroxyl radical from Superoxide ani nand hydrogen peroxide generated during...Superoxide ani n(O2' ), hydrogen peroxide (H2O2...monophosphate shunt, production of NAD(P)H, and...cytotoxicity. MATERIALS AND METHODS Reagents Crocidolite...

Lee A. Goodglick and Agnes B. Kane

1986-11-01T23:59:59.000Z

363

Effect of gamma interferon on hydrogen peroxide production by cultured mouse peritoneal macrophages.  

Science Journals Connector (OSTI)

...increase in hydrogen peroxide production and only in...assayed by the method of Pick and...Immunol. Methods 38:161-170...superoxide and hydrogen peroxide production by macrophages...Immunol. Methods 46:211-226...

A K Sharp; D K Banerjee

1986-11-01T23:59:59.000Z

364

Solution Structure of Mouse Cripto CFC Domain and Its Inactive Variant Trp107Ala  

Science Journals Connector (OSTI)

a?Values are the average ± standard deviation for the ensemble of the best 40 structures obtained by REDAC strategy. ... To improve convergence, the redundant dihedral angle constraints (REDAC)54 strategy was also employed, and 100 more structures were calculated by carrying out five REDAC cycles for the analogues. ... The REDAC structures are indeed more compatible with the experimentally obtained data. ...

Luisa Calvanese; Angela Saporito; Daniela Marasco; Gabriella D'Auria; Gabriella Minchiotti; Carlo Pedone; Livio Paolillo; Lucia Falcigno; Menotti Ruvo

2006-11-01T23:59:59.000Z

365

The architecture of the mouse trigeminal-facial brainstem : disynaptic circuitry, genomic organization, and follicle mechanics  

E-Print Network [OSTI]

MicroBrightField Inc. , Williston, VT). Brain traces wereMicroBrightField, Inc. , Williston, VT) according to the

Matthews, David William; Matthews, David William

2012-01-01T23:59:59.000Z

366

Behavior of Tritium in the Mouse Body After Oral Intake and Estimation of the Absorbed Dose  

Science Journals Connector (OSTI)

Biology / Proceedings of the Sixth International Conference on Tritium Science and Technology Tsukuba, Japan November 12-16, 2001

Masahiro Saito

367

E-Print Network 3.0 - adult mouse nervous Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

at Manoa Collection: Environmental Sciences and Ecology 39 November 4, 2009 6.16 The sonic hedgehog gene is shown by in situ hybridization to be expressed in the chick nervous...

368

E-Print Network 3.0 - adult mouse subventricular Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Medicine 82 Neuron, Vol. 39, 937950, September 11, 2003, Copyright 2003 by Cell Press Sonic Hedgehog Is Required for Progenitor Cell Summary: and Gli1 Expression in the Adult...

369

E-Print Network 3.0 - adult mouse hippocampus Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

adult hippocampus. Rat hip- pocampal progenitors proliferated when cultured in Shh, and Sonic hedgehog... cells. We found high expression of the Shh receptor Patched in both the...

370

Inhibition of Tumor Promotion in Mouse Skin by 1?,25-Dihydroxyvitamin D3  

Science Journals Connector (OSTI)

...Center, Nijmegen, Netherlands; Diakonessen Hospital Utrecht, Utrecht, Netherlands; Canisius-Wilhelmina Hospital, Nijmegen...therapy1. It is questionable whether these results can be applied to patients undergoing a mastectomy2. Trial design...

Kazuhiro Chida; Hiroki Hashiba; Masafumi Fukushima; Tatsuo Suda; and Toshio Kuroki

1985-11-01T23:59:59.000Z

371

The Interaction of Carcinogenic Hydrocarbons with Tissues VII. Fractionation of Mouse Skin Proteins  

Science Journals Connector (OSTI)

...Inferaction of Hydrocarbons with Tissues. VII...separation of the water- soluble proteins...in Chart 1, and water-soluble proteins...Interaction of Hydrocarbons with Tissues. VII...noncarcinogenic hydrocarbons could be detected...electrophoretic separation of water-soluble proteins...

G. Rodman Davenport; C. W. Abell; and Charles Heidelberger

1961-06-01T23:59:59.000Z

372

Ceramide Generation in Response to Photodynamic Treatment of L5178Y Mouse Lymphoma Cells  

Science Journals Connector (OSTI)

...N-acetyl-dihydrosphingosine; DAG, diacylglycerol; DETAPAC, diethylenetriamine pentaacetic acid; LED, light-emitting diode; LSM, low-serum medium; LY-R, L5178Y-R; PDT, photodynamic treatment; SAPK, stress-activated protein...

Duska Separovic; Jin He; Nancy L. Oleinick

1997-05-01T23:59:59.000Z

373

Response and Resistance to NF-?B Inhibitors in Mouse Models of Lung Adenocarcinoma  

E-Print Network [OSTI]

Lung adenocarcinoma is a leading cause of cancer death worldwide. We recently showed that genetic inhibition of the NF-?B pathway affects both the initiation and the maintenance of lung cancer, identifying this pathway as ...

Xue, Wen

374

Functional recovery with recombinant human IGF1 treatment in a mouse model of Rett Syndrome  

E-Print Network [OSTI]

Rett Syndrome is a neurodevelopmental disorder that arises from mutations in the X-linked gene methyl-CpG binding protein 2 (MeCP2). MeCP2 has a large number of targets and a wide range of functions, suggesting the hypothesis ...

Garcia, Rodrigo

375

Imaging vasodynamics in the awake mouse brain with two-photon Andy Y. Shih1  

E-Print Network [OSTI]

magnetic resonance imaging PoRTS = polished and reinforced thinned skull RBC = red blood cell TPLSM = two-to-moment energy demands of neuronal computation in the mammalian brain are high, yet the brain has limited reserves of energy [1, 2]. A solution to this dilemma calls for a vascular system that functions

Kleinfeld, David

376

Analysis of pluripotent mouse stem cell proteomes : insights into post- transcriptional regulation of pluripotency and differentiation  

E-Print Network [OSTI]

R. N. , Shen, Z. , Tachikawa, K. , Briggs, S.P. QuantitativeZ. , Drenthe, L, Kooistra S. L. Tachikawa, K. , Eggan, B. E.Chris van Schie, Kiyoshi Tachikawa, Amanda Mason, Kelly

O'Brien, Robert Norman

2010-01-01T23:59:59.000Z

377

Inhibition of Mouse Sarcoma 180 by Polysaccharides from Lentinus edodes (Berk.) Sing.  

Science Journals Connector (OSTI)

... (Berk.) S.Ito et Imai, Armillaria edodes (Berk.) Sacc. and Cortinellus shiitake (Takeda) P. Henn), an edible ... (Takeda) P. Henn), an edible mushroom found in Japan and other parts of the Far East. Four poly-saccharide preparations, ...

GORO CHIHARA; YUKIKO MAEDA; JUNJI HAMURO; TAKUMA SASAKI; FUMIKO FUKUOKA

1969-05-17T23:59:59.000Z

378

Mechanisms of entry of poliovirus into the central nervous system in a mouse model  

E-Print Network [OSTI]

) Experimental mice did not develop paralysis when Lansing type II poliovirus was injected intravenously, 2) Using electron microscopy nopoliovirus-like particles were detected in either the endothelial cells or inflammatory cells, 3) Using immunofluorescence...

Ting, Jing-Wen

2012-06-07T23:59:59.000Z

379

Abstract 2872: Effects of ethanol and folate on peripubertal mouse mammary gland  

Science Journals Connector (OSTI)

...Long-term exposure of breast cell lines to ethanol affects the transcriptional signature...mammospheres. Here we aimed to determine whether ethanol, or its metabolite, acetaldehyde, stimulate...cell lines were incubated /- 25 mM ethanol and subjected to DNA microarray analysis...

Patricia A. Masso-Welch; Menachem E. Tobias; Shyam C. Vasantha Kumar; Judith Tamburlin; Stephen T. Koury

2011-01-10T23:59:59.000Z

380

Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure  

Science Journals Connector (OSTI)

...litters per treatment group. Different letter denotes P ? 0.05 independent of maternal...0001 0.0046 ± 0.0001 Different letters...1296 . 22 Barker DJP ( 1998 ) Mothers, Babies and Health in Later Life ( Churchill Livingstone...

Adam J. Watkins; Duncan Platt; Tom Papenbrock; Adrian Wilkins; Judith J. Eckert; Wing Yee Kwong; Clive Osmond; Mark Hanson; Tom P. Fleming

2007-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

WING TIP ANATOMY AND AERODYNAMICS IN FLYING National Museum of Natural History, Department of Vertebrate Zoology,  

E-Print Network [OSTI]

WING TIP ANATOMY AND AERODYNAMICS IN FLYING SQUIRRELS National Museum of Natural History aerodynamically sophisticat- back into a curve by the tibiocarpalis mus- ed. They are able to modify shape

Mathis, Wayne N.

382

E-Print Network 3.0 - andres trikkel rein Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mark, snegler, mus, br og andre... for bevare eller ke vannstanden, hytta gir god beskyttelse mot andre dyr inngangen er under vann... i Oslo 5 MONTER 30. REIN OG ULV...

383

E-Print Network 3.0 - andres kuusik rein Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

mark, snegler, mus, br og andre... for bevare eller ke vannstanden, hytta gir god beskyttelse mot andre dyr inngangen er under vann... i Oslo 5 MONTER 30. REIN OG ULV...

384

Protons Act as a Transmitter for Muscle Contraction in C. elegans  

E-Print Network [OSTI]

and pbo-6 genes encode subunits of a ``cys- loop'' proton-gated cation channel required for mus- cles. Usually, classical neurotransmitters are stored in synaptic vesi- cles. Calcium stimulates the fusion

Cooper, Robin L.

385

Ocean Dynamics Theoretical, Computational and  

E-Print Network [OSTI]

-tracking- derived currents in May­June 2009 under three different wind conditions, we reproduced the June 2009 and the Woods Hole Oceanographic Institution RE- MUS autonomous underwater vehicle Operations Group who

Chen, Changsheng

386

Tuning the photophysical properties of amidophosphine complexes of copper  

E-Print Network [OSTI]

A series of monomeric copper complexes that allow for the tuning of the emission properties is reported. Luminescence lifetimes up to 150 [mu]s are observed in benzene solution at ambient temperature, which are comparable ...

Mickenberg, Seth F. (Seth Fox)

2009-01-01T23:59:59.000Z

387

Delayed prezygotic isolating mechanisms: evolution with a twist  

Science Journals Connector (OSTI)

...ation should be a plausible, rapid process. Prezygotic reproductive isolation as...escape mechanism from these attractors, a process known as evol- utionary branching: by...rinversion bei Laciniaria biplicata. Mtg. Mitt. Hamb. Zool. Mus. Inst. 51...

2002-01-01T23:59:59.000Z

388

The complete mitochondrial genome of the enigmatic bigheaded turtle (Platysternon): description of unusual genomic features and the reconciliation of phylogenetic hypotheses based on mitochondrial and nuclear DNA  

E-Print Network [OSTI]

ES: Phylogeny of the chelydrid turtles: a study of sharedOrdosemys, an Early Cretaceous turtle from Inner Mongolia,articulations of living turtles. Bull. Am. Mus. Nat. Hist.

Parham, James F.; Feldman, Chris R.; Boore, Jeffrey L.

2005-01-01T23:59:59.000Z

389

131st AAAS Annual Meeting  

Science Journals Connector (OSTI)

...spectrum as an unex-plored natural resource for economic...develop-ment of museums of natural history and anthropology...G. E. L. Owen (Corpus Christi College, Oxford, England...Brunswick). Man's natural mus-cle system, G...

1964-12-04T23:59:59.000Z

390

E-Print Network 3.0 - adult rat sacral Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

capensis) from Cape Town. Used with the permission of the pho... . Georychus capensis (Pallas, 1778) Cape Mole-rat Mus capensis Pallas, 1778:172. Type locality ... Source: Hayssen,...

391

PRIX PHOTO DU MUSUM NATIONAL D'HISTOIRE NATURELLE BOURSE PROFESSIONNELLE 2014 -57 RUE CUVIER, 75005 PARIS  

E-Print Network [OSTI]

PRIX PHOTO DU MUS�UM NATIONAL D'HISTOIRE NATURELLE BOURSE PROFESSIONNELLE 2014 - 57 RUE CUVIER énoncées à l'article 4 du Règlement, à : Prix Photo Bourse Professionnelle du Muséum national d professionnelle du Prix Photo du Muséum national d'Histoire naturelle, et en être détenteur des droits d'auteur. J

392

The Empire of French Imperial Art: Jacques-Louis David and the Napoleonic Regime, 1799 to 1812  

E-Print Network [OSTI]

on canvas 151x214cm, The National Gallery of Canada, Ottawa ............................................................. 92 ix FIGURE Page 13 Jacques-Louis David, The Death of Marat, 1793 oil on canvas, 165x128, Mus?e d'art ancien, Brussels..., 203.9x125.1 cm The National Gallery of Art, Washington D.C .................................................. 100 26 Jacques-Louis David, Leonidas at Thermopylae, 1814 oil on canvas, 395x531 cm, Mus?e de Louvre, Paris...

Bajew, Brian C

2010-04-30T23:59:59.000Z

393

C3Bio.org - Login  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Penguin Chicken Suitcase Teapot Mouse Please leave this field blank. required Problem: required...

394

Anti-inflammatory capabilities of compounds from marine bacteria in a mouse model of allergic inflammation and asthma  

E-Print Network [OSTI]

specific sequences of synthetic DNA that prompt the immuneantibody to IgE and synthetic bacterial DNA sequences whichsynthetic drugs are based on natural sequences of bacterial DNA

Strangman, Wendy K.

2007-01-01T23:59:59.000Z

395

CXC Receptor 1 and 2 and Neutrophil Elastase Inhibitors Alter Radiation-induced Lung Disease in the Mouse  

SciTech Connect (OSTI)

Purpose: We previously reported increased numbers of neutrophils to be associated with the development of the radiation-induced lung responses of alveolitis (pneumonitis) and fibrosis in mice. In the present study we investigated whether CXC receptor 1 and 2 antagonism with DF2156A, a small molecule inhibitor of neutrophil chemotaxis, or the neutrophil elastase inhibitor sivelestat decreases the lung response to irradiation. Methods and Materials: KK/HIJ mice received 14 Gy whole-thorax irradiation, and a subset of them received drug treatment 3 times per week from the day of irradiation until they were killed because of respiratory distress symptoms. Results: Irradiated mice receiving sivelestat survived 18% longer than did mice receiving radiation alone (73 vs 60 days for female mice, 91 vs 79 days for male mice), whereas postirradiation survival times did not differ between the group of mice receiving DF2156A and the radiation-only group. The numbers of neutrophils in lung tissue and in bronchoalveolar lavage fluid did not differ among groups of irradiated mice, but they significantly exceeded the levels in unirradiated control mice. The extent of alveolitis, assessed histologically, did not differ between irradiated mice treated with either drug and those receiving radiation alone, when assessed at the end of the experiment, but it was significantly reduced, as were the neutrophil measures, in sivelestat-treated mice at the common kill time of 60 days after irradiation. Mice treated with radiation and DF2156A developed significantly less fibrosis than did mice receiving radiation alone, and this difference was associated with decreased expression of interleukin-13 in lung tissue. Conclusions: We conclude that neutrophil elastase inhibition affects alveolitis and prolongs survival, whereas CXCR1/2 antagonism reduces radiation-induced fibrotic lung disease in mice without affecting the onset of distress.

Fox, Jessica [Department of Medicine and the Meakins-Christie Laboratories, McGill University, Montreal, Quebec (Canada)] [Department of Medicine and the Meakins-Christie Laboratories, McGill University, Montreal, Quebec (Canada); Haston, Christina K., E-mail: christina.haston@mcgill.ca [Department of Medicine and the Meakins-Christie Laboratories, McGill University, Montreal, Quebec (Canada)

2013-01-01T23:59:59.000Z

396

Disruption of Early Pregnancy in the CF-1 Mouse: Impacts of Triclosan Alone and in Combination with Bisphenol-A.  

E-Print Network [OSTI]

?? Triclosan is an antimicrobial additive found in a number of personal care and household products. Widely detected in humans, the compound has been given… (more)

Crawford, Brent R

2012-01-01T23:59:59.000Z

397

Immunodeficient Mouse Models with Different Disease Profiles by In Vivo Infection with the Same Clinical Isolate of Enterovirus 71  

Science Journals Connector (OSTI)

...analysis by an ABI 7500 system with a Power SYBR green PCR master kit (both from Applied...71 isolated from a patient with hand, foot and mouth disease. Arch. Virol. 56...and H-S Wei. 2011. Severe hand-foot-mouth disease: a report of 3 autopsied...

Chun-Che Liao; An-Ting Liou; Ya-Shu Chang; Szu-Yao Wu; Chih-Shin Chang; Chien-Kuo Lee; John T. Kung; Pang-Hsien Tu; Ya-Yen Yu; Chi-Yung Lin; Jen-Shiou Lin; Chiaho Shih

2014-08-20T23:59:59.000Z

398

A Gnotobiotic Mouse Model Demonstrates That Dietary Fiber Protects against Colorectal Tumorigenesis in a Microbiota- and Butyrate-Dependent Manner  

Science Journals Connector (OSTI)

...mitochondria and accounts for 70% of the energy used by normal colonocytes (11), and...which use butyrate as their primary energy source (11), colorectal cancer cells...butyrate, which serves as the preferred energy source of normal colonocytes and supports...

Dallas R. Donohoe; Darcy Holley; Leonard B. Collins; Stephanie A. Montgomery; Alan C. Whitmore; Andrew Hillhouse; Kaitlin P. Curry; Sarah W. Renner; Alicia Greenwalt; Elizabeth P. Ryan; Virginia Godfrey; Mark T. Heise; Deborah S. Threadgill; Anna Han; James A. Swenberg; David W. Threadgill; Scott J. Bultman

2014-12-01T23:59:59.000Z

399

Complementary DNA Cloning, Messenger RNA Expression, and Induction of ?-Class Glutathione S-Transferases in Mouse Tissues  

Science Journals Connector (OSTI)

...suggested the presence of at least four or five a-class GST genes...CA). Two replica plaque lifts were made on Nytran filters...5-min intervals for five time points. Mung bean nuclease was then...six colonies from each time point were grown in 3 ml of Luria...

Timo M. Buetler and David L. Eaton

1992-01-15T23:59:59.000Z

400

Changes of the Hepatic Proteome in Hepatitis B-Infected Mouse Model at Early Stages of Fibrosis  

Science Journals Connector (OSTI)

Mass signals were then used for database searching using the MASCOT peptide fingerprinting search program (Matrix Science, Boston, MA), available on the Internet. ... Tonge, R.; Shaw, J.; Middleton, B.; Rowlinson, R.; Rayner, S.; Young, J.; Pognan, F.; Hawkins, E.; Currie, I.; Davison, M. Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology Proteomics 2001 1 3 377 396 ... abuse starting at a younger age. ...

Daniela Spano; Flora Cimmino; Mario Capasso; Fulvio D’Angelo; Nicola Zambrano; Luigi Terracciano; Achille Iolascon

2008-05-22T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Characterization of the mouse transforming growth factor alpha gene: its expression during eyelid development and in waved 1 tissues  

Science Journals Connector (OSTI)

...Fig. 3). Scanning laser densitometry and phosphoimager...I I leo B DB PVAAAVVS HF NKC P D8 HT QYC PEG TC...segment (32). Eyelid Fusion Is Consistently Delayed...prenatal eyelid growth and fusion. To test this hypothesis...wild-type embryos displayed fusion of the opposing epithelia...

EA Berkowitz; KB Seroogy; JA Schroeder; WE Russell; EP Evans; RF Riedel; HK Phillips; CA Harrison; DC Lee; and NC Luetteke

1996-09-01T23:59:59.000Z

402

Microcircuits for Night Vision in Mouse Retina Yoshihiko Tsukamoto,1 Katsuko Morigiwa,2 Mika Ueda,1 and Peter Sterling3  

E-Print Network [OSTI]

glutamate receptors (Hack et al., 1999, 2001). We show that this rod­cone bipolar cell sends an axon from rods to processes that express ionotropic glutamate recep- tors (iGluRs) (Hack et al., 1999, 2001

Pennsylvania, University of

403

Potato Chip Intake Increases Ascorbic Acid Levels and Decreases Reactive Oxygen Species in SMP30/GNL Knockout Mouse Tissues  

Science Journals Connector (OSTI)

Kondo, Y.; Higashi, C.; Iwama, M.; Ishihara, K.; Handa, S.; Mugita, H.; Maruyama, N.; Koga, H.; Ishigami, A.Bioavailability of vitamin C from mashed potatoes and potato chips after oral administration in healthy Japanese men Br. ... Bioavailability of vitamin C from mashed potatoes and potato chips after oral administration in healthy Japanese men ... After overnight fasting, five healthy Japanese men between 22 and 27 years of age consumed 87 g mashed potatoes and 282 g potato chips. ...

Yoshitaka Kondo; Rui Sakuma; Megumi Ichisawa; Katsuyuki Ishihara; Misako Kubo; Setsuko Handa; Hiroyuki Mugita; Naoki Maruyama; Hidenori Koga; Akihito Ishigami

2014-09-02T23:59:59.000Z

404

The expression of neuronal nitric oxide synthase during postnatal development in the leaner and tottering mouse cerebella  

E-Print Network [OSTI]

Tottering and leaner mice carry different mutations in the portion of their genetic information (tottering locus) that encodes for the ??[A] subunit of P/Q-type voltage-gated calcium ion channels. These channels, located on the cell membrane...

Zeve, Daniel Richard

2013-02-22T23:59:59.000Z

405

Biomechanics of common carotid arteries from mice heterozygous for mgR, the most common mouse model of Marfan syndrome  

E-Print Network [OSTI]

Marfan syndrome, affecting approximately one out of every 5,000 people, is characterized by abnormal bone growth, ectopia lentis, and often-fatal aortic dilation and dissection. The root cause is a faulty extracellular matrix protein, fibrillin-1...

Taucer, Anne Irene

2009-05-15T23:59:59.000Z

406

Cerebellar Purkinje cell death in the P/Q -type voltage-gated calcium ion channel mutant mouse, leaner  

E-Print Network [OSTI]

inducing an inflammatory response in the tissue surrounding the affected cell (Sastry and Rao, 2000; Yuan and Yankner, 2000; Yuan, et al., 2003). Programmed cell death is an essential requirement for normal neurodevelopment and a key component of many...

Frank-Cannon, Tamy Catherine

2006-04-12T23:59:59.000Z

407

Transcription factors SOX4 and SOX11 function redundantly to regulate the development of mouse retinal ganglion cells  

E-Print Network [OSTI]

Lerner Institute, Cleveland Clinic, Cleveland, OH 44195 4 College of Life and Environmental Sciences layer while deletion of Brn3b has no effect on the expression of Sox4 and Sox11. Taken together layers: photoreceptors (rods and cones) constitute the outer nuclear layer (ONL); horizontal, bipolar

408

Immunodeficient Mouse Models with Different Disease Profiles by In Vivo Infection with the Same Clinical Isolate of Enterovirus 71  

Science Journals Connector (OSTI)

...VP1-specific RNA and protein in muscle, brain, and spinal cord, were compared side...Purkinje layer of cerebellar cortex, pons, brain stem, and spinal cord, (iii) amplification...hand-foot-and-mouth disease (HFMD), uncomplicated brain stem encephalitis, severe dysregulation...

Chun-Che Liao; An-Ting Liou; Ya-Shu Chang; Szu-Yao Wu; Chih-Shin Chang; Chien-Kuo Lee; John T. Kung; Pang-Hsien Tu; Ya-Yen Yu; Chi-Yung Lin; Jen-Shiou Lin; Chiaho Shih

2014-08-20T23:59:59.000Z

409

Dose-Response and Large Relative Biological Effectiveness of Fast Neutrons with Regard to Mouse Fetal Cerebral Neuron Apoptosis  

Science Journals Connector (OSTI)

......visualize apoptotic cells, we used the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method of immunohistochemical staining...42: 611620. 13. Hendry, J. H., Potten, C. S. and Merritt, A. (1995) Apoptosis induced by high- and low-LET radiations......

Yuka Ishida; Yasushi Ohmachi; Yukiko Nakata; Takeshi Hiraoka; Tsuyoshi Hamano; Shinji Fushiki; Toshiaki Ogiu

2006-01-01T23:59:59.000Z

410

A 78-kilobase region of mouse chromosome 3 contains salivary and pancreatic amylase genes and a pseudogene  

Science Journals Connector (OSTI)

...sequences by probing a Southern blot of the subcloned DNA with nick-translated genomic DNA and also by using the labeled fragment...Himeno, S. & Matsubara, K. (1984) Gene 28, 263-270. 5. Merritt, A. D. & Karn, R. C. (1977) in Advances in Human Genetics...

K Wiebauer; D L Gumucio; J M Jones; R M Caldwell; H T Hartle; M H Meisler

1985-01-01T23:59:59.000Z

411

Arf gene loss enhances oncogenicity and limits imatinib response in mouse models of Bcr-Abl-induced acute lymphoblastic leukemia  

Science Journals Connector (OSTI)

...it contributes to drug resistance in human patients therefore merit consideration. The manner by which Arf...were assayed by terminal transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) by using a APO-BRDU KIT (BD Biosciences...

Richard T. Williams; Martine F. Roussel; Charles J. Sherr

2006-01-01T23:59:59.000Z

412

Identification of nucleotide pyrophosphatase/alkaline phosphodiesterase I activity associated with the mouse plasma cell differentiation antigen PC-1.  

Science Journals Connector (OSTI)

...the cDNA insert from ElAl radiolabeled by nick-translation with [a-32P]dCTP. Hybridization...48 hr. The blot was hybridized with the nick- translated 2.7-kilobase cDNA isolated...glycosyltransferases. This investigation was supported by Merit Review Grants from the Department of Veterans...

N F Rebbe; B D Tong; E M Finley; S Hickman

1991-01-01T23:59:59.000Z

413

The role of p53 in normal development and teratogen-induced apoptosis and birth defects in mouse embryos  

E-Print Network [OSTI]

....................... 21 1.2.2.1. Hyperthermia ..................................................................... 21 1.2.2.2. Cyclophosphamide ............................................................ 25 1.2.2.3. Other Teratogens... .............. 14 Figure 1.2 Metabolism of Cyclophosphamide .................................................... 26 Figure 1.3 Comparison of Cell Death Machinery in Mammals to the C. elegans System...

Hosako, Hiromi

2009-05-15T23:59:59.000Z

414

Inhibitory actions of Ah receptor agonists and indole-containing compounds in breast cancer cell lines and mouse models  

E-Print Network [OSTI]

The aryl hydrocarbon receptor (AhR) binds synthetic and chemoprotective phytochemicals, and research in this laboratory has developed selective AhR modulators (SAhRMs) for treatment of breast cancer. Activation of the AhR through agonists...

Walker, Kelcey Manae Becker

2005-08-29T23:59:59.000Z

415

Biochemical analysis of heat-resistant mouse tumor cell strains: a new member of the HSP70 family.  

Science Journals Connector (OSTI)

...thermostable form (7). Heat increases the fluidity...membranes (28), leading to loss of their selective permeability...of 10 months without loss of heat resistance or change...on the intracellular distribution of heat-shock protein 70...

R L Anderson; I Van Kersen; P E Kraft; G M Hahn

1989-08-01T23:59:59.000Z

416

Maternal diet-induced obesity programmes cardiovascular dysfunction in adult male mouse offspring independent of current body weight  

E-Print Network [OSTI]

heart per- fusion, offspring at 12weeks of age remained fed andwere culled by cervical dislocation. The hearts used in the Langendorff heart perfusion were fixed post procedure, whereas the heart of its littermate was frozen. Blood was allowed to clot... and the heart was rapidly excised and immediately placed into ice-cold Krebs-Henseleit bicarbonate (KHB) buf- fer (120mM NaCl, 4.7mM KCl, 1.2mM MgSO2·7H2O, 1.2mMKH2PO4, 25mMNaHCO3, 10mMglucose, and1.3mM CaCl2·2H2O). The heart was cannulated via the aorta and per...

Blackmore, Heather L.; Niu, Youguo; Fernandez-Twinn, Denise S.; Tarry-Adkins, Jane L.; Giussani, Dino A.; Ozanne, Susan E.

2014-07-18T23:59:59.000Z

417

Biodiesel versus diesel exposure: Enhanced pulmonary inflammation, oxidative stress, and differential morphological changes in the mouse lung  

SciTech Connect (OSTI)

The use of biodiesel (BD) or its blends with petroleum diesel (D) is considered to be a viable approach to reduce occupational and environmental exposures to particulate matter (PM). Due to its lower particulate mass emissions compared to D, use of BD is thought to alleviate adverse health effects. Considering BD fuel is mainly composed of unsaturated fatty acids, we hypothesize that BD exhaust particles could induce pronounced adverse outcomes, due to their ability to readily oxidize. The main objective of this study was to compare the effects of particles generated by engine fueled with neat BD and neat petroleum-based D. Biomarkers of tissue damage and inflammation were significantly elevated in lungs of mice exposed to BD particulates. Additionally, BD particulates caused a significant accumulation of oxidatively modified proteins and an increase in 4-hydroxynonenal. The up-regulation of inflammatory cytokines/chemokines/growth factors was higher in lungs upon BD particulate exposure. Histological evaluation of lung sections indicated presence of lymphocytic infiltrate and impaired clearance with prolonged retention of BD particulate in pigment laden macrophages. Taken together, these results clearly indicate that BD exhaust particles could exert more toxic effects compared to D. - Highlights: • Exposure of mice to BDPM caused higher pulmonary toxicity compared to DPM. • Oxidative stress and inflammation were higher in BD vs to D exposed mice. • Inflammatory lymphocyte infiltrates were seen only in lungs of mice exposed to BD. • Ineffective clearance, prolonged PM retention was present only after BD exposure.

Yanamala, Naveena, E-mail: wqu1@cdc.gov [Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States); Hatfield, Meghan K., E-mail: wla4@cdc.gov [Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States); Farcas, Mariana T., E-mail: woe7@cdc.gov [Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States); Schwegler-Berry, Diane [Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States); Hummer, Jon A., E-mail: qzh3@cdc.gov [Office of Mine Safety and Health Research/NIOSH/CDC, Pittsburgh, PA 15236 (United States); Shurin, Michael R., E-mail: shurinmr@upmc.edu [Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA (United States); Birch, M. Eileen, E-mail: mib2@cdc.gov [NIOSH/CDC, 4676 Columbia Parkway, Cincinnati, OH 45226 (United States); Gutkin, Dmitriy W., E-mail: dwgutkin@hotmail.com [Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA (United States); Kisin, Elena, E-mail: edk8@cdc.gov [Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States); Kagan, Valerian E., E-mail: kagan@pitt.edu [Department of Environmental and Occupational Health, University of Pittsburgh, PA (United States); Bugarski, Aleksandar D., E-mail: zjl1@cdc.gov [Office of Mine Safety and Health Research/NIOSH/CDC, Pittsburgh, PA 15236 (United States); Shvedova, Anna A., E-mail: ats1@cdc.gov [Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States); Department Physiology and Pharmacology, WVU, Morgantown, WV 26505 (United States)

2013-10-15T23:59:59.000Z

418

Formation and Removal of Specific Acetylaminofluorene-DNA Adducts in Mouse and Human Cells Measured by Radioimmunoassay  

Science Journals Connector (OSTI)

...sulfoxide for 1 hr at 37 .For removal experiments, one half of the...percentage of inhibition was always additive (Chart 1B), demonstrating...determination of binding and removal of C-B adducts from the DNAof...incubate for 23 hr after the removal of the carcinogen and indicates...

Miriam C. Poirier; Marc A. Dubin; and Stuart H. Yuspa

1979-04-01T23:59:59.000Z

419

Identification of the glycosaminoglycan-attachment site of mouse invariant-chain proteoglycan core protein by site-directed mutagenesis  

Science Journals Connector (OSTI)

...pressing Ia a and f3 chains but not Ii (BADH3.1) (14) were supertransfected with the wild-type and mutant constructs. A wtI a, 3 only only I 1 p25- l 2 B lj-CS[9 a- w p31 Ij 13: a,3 a ,1 +wtI1 +1i-010201 As in the COS-7 system, precipitation...

J Miller; J A Hatch; S Simonis; S E Cullen

1988-01-01T23:59:59.000Z

420

The characterization of a mouse model of transient stroke using ex vivo MR microscopy and in vivo MR imaging  

E-Print Network [OSTI]

Disrupted blood-brain barrier after an ischemic attack can cause vasogenic edema and increase the risk of hemorrhagic transformation. Therefore, early detection and monitoring of BBB damage is important in the pathological ...

Huang, Shuning, Ph. D. Massachusetts Institute of Technology

2009-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

High Frequency and Heterogeneous Distribution of p53 Mutations in Aflatoxin B1-induced Mouse Lung Tumors  

Science Journals Connector (OSTI)

...effects of temperature in the fever...transmembrane distributions of radiolabeled...the core body temperature of experimental...transmembrane distributions of TPMP and...transmembrane distributions of drugs transported...Dr. Peck-Sun Lin in the...the core body temperature of laboratory...

Andrew S. Tam; Julie F. Foley; Theodora R. Devereux; Robert R. Maronpot; and Thomas E. Massey

1999-08-01T23:59:59.000Z

422

MouseLight: Bimanual Interactions on Digital Paper Using a Pen and a Spatially-Aware Mobile Projector  

E-Print Network [OSTI]

Projector Hyunyoung Song1,2,3 , Francois Guimbretiere2,3 , Tovi Grossman1 , George Fitzmaurice1 1 Autodesk Research 210 King St. East, Toronto, ON, M5A 1J7 Canada {firstname.lastname}@autodesk.com 2 University

Toronto, University of

423

Heat Shock Protein Synthesis and Cell Survival in Clones of Normal and Simian Virus 40-transformed Mouse Embryo Cells  

Science Journals Connector (OSTI)

...speculate that this abnormal use of energy-producing substrates by transformed...synthesis of the same polypep tides in starved T. pyriformis...hamster fibroblasts. Proc. major energy source for cultured HeLacells...47: 5188-5192,1981. tides induce heat shock proteins...

Rawhi A. Omar and Karl W. Lanks

1984-09-01T23:59:59.000Z

424

Development of an In Vitro Activity Assay as an Alternative to the Mouse Bioassay for Clostridium botulinum Neurotoxin Type A  

Science Journals Connector (OSTI)

...recombinant BoNT-A light chain (LcA) was also obtained from List...from the U.S. Department of Agriculture, Agricultural Research Service...buffer. Assays of BoNT-A or LcA protease activities. Recombinant...food samples to be spiked with LcA were diluted in 20 mM HEPES...

Reuven Rasooly; Paula M. Do

2008-05-30T23:59:59.000Z

425

Evaluation of the Effects of Green Tea Extracts on Bone Homeostasis in the Ts65Dn Down Syndrome Mouse Model  

E-Print Network [OSTI]

Evaluation of the Effects of Green Tea Extracts on Bone Homeostasis in the Ts65Dn Down Syndrome- gallate (EGCG), an aromatic polyphenol found in high concentrations in green tea, is a known inhibitor

Zhou, Yaoqi

426

J Physiol . Author manuscript In vivo expression of G-protein beta1gamma2 dimer in adult mouse  

E-Print Network [OSTI]

of glucose uptake and of ion transport and membrane excitability (for review see (Jean-Baptiste ,et al localization in the transverse-tubulevivo 1 2 membrane. Membrane current and indo-1 fluorescence measurements ; Ion Channel Gating ; physiology ; Mice ; Muscle Contraction ; physiology ; Muscle Fibers, Skeletal

Boyer, Edmond

427

AAV serotype-1 mediates early onset of gene expression in mouse hearts and results in better therapeutic effect  

E-Print Network [OSTI]

transfer. 2,3 In an acute heart attack, metabolic changesprevious history of heart attack or blockage of the coronary

2006-01-01T23:59:59.000Z

428

Microtubule mediation of cytoplasmic and nuclear maturation during the early stages of resumed meiosis in cultured mouse oocytes  

Science Journals Connector (OSTI)

...passed through the peri- nuclear mitochondrial layer...concentrate the limited energy- producing capacity...that a deficiency of nuclear envelope-associated...oocytes is due to an energy deficiency rather than...through small breaks in the nuclear envelope (19). Although...

J Van Blerkom

1991-01-01T23:59:59.000Z

429

The Ligand Binding Domain of GCNF Is Not Required for Repression of Pluripotency Genes in Mouse Fetal Ovarian Germ Cells  

E-Print Network [OSTI]

In mice, successful development and reproduction require that all cells, including germ cells, transition from a pluripotent to a differentiated state. This transition is associated with silencing of the pluripotency genes ...

Okumura, Leah M.

430

Systems analysis of primary Sjogren's syndrome pathogenesis in salivary glands identifies shared pathways in human and a mouse model  

E-Print Network [OSTI]

Medical Center at Groningen, were at least 21 years old, andSurgery, University Medical Center Groningen, Universityof Groningen, P.O. Box 30.001, Groningen, the Netherlands. 3

2012-01-01T23:59:59.000Z

431

Attempts to Target Antitumor Drugs toward Opioid Receptor-rich Mouse Tumor Cells with Enkephalin-Ellipticinium Conjugates  

Science Journals Connector (OSTI)

...which cannot cross the plasma membrane without site-directed...which cannot cross the plasma membrane without site-directed...which cannot cross the plasma membrane without site-directed...at 37 Cin a humidified atmosphere of 5% CO2 and 95% air...sonication (15 s at 75 W, 20 KHz; Sonifier B-12; Branson...

Pascal Rigaudy; Jean-Yves Charcosset; Christiane Garbay-Jaureguiberry; Alain Jacquemin-Sablon; and Bernard P. Roques

1989-04-01T23:59:59.000Z

432

Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.  

Science Journals Connector (OSTI)

...Bennett (Univer- sity of Missouri, Columbia). Probe KS13A...aperture Plan Neofluar oil-immersion objective...aperture Plan Apochromat oil-immersion objective...characterized by two equally heavy middle bands, designated...bands, especially the heavy E band (see Fig. 1E...

A L Boyle; S G Ballard; D C Ward

1990-01-01T23:59:59.000Z

433

Characterization of the 3' terminal 42 nucleotide host protein binding element of the mouse hepatitis virus 3' untranslated region  

E-Print Network [OSTI]

-quantitative RT-PCR of negative strand B36 ATW, ATW3', and ATW5' DI?s generated in DI replication assays???????. 98 24 Panel A shows an example of construction of the template for infectious clone generation using the enzyme Esp 3I??........?.??? 102...-quantitative RT-PCR of negative strand B36 ATW, ATW3', and ATW5' DI?s generated in DI replication assays???????. 98 24 Panel A shows an example of construction of the template for infectious clone generation using the enzyme Esp 3I??........?.??? 102...

Johnson, Reed Findley

2004-09-30T23:59:59.000Z

434

Role of Tumor Necrosis Factor and Interleukin 1 in ?-Interferon-promoted Activation of Mouse Tumoricidal Macrophages  

Science Journals Connector (OSTI)

...activation could be blocked by: (a) heating the mole cules (Groups F and G) or...8 0.4 2.9 0.6 4.211.5 ND 2.Oil.2 2.3+1.0 57.112.0 ND 16...Tushinski, R. J.. Mochizuki. D. Y.. Price, V. L., <'antri-li. M. A...

Kazuyoshi Hori; Enrico Mihich; and M. Jane Ehrke

1989-05-15T23:59:59.000Z

435

Identification and Evaluation of Brucella Recombinant Outer Membrane Proteins as Subunit Vaccinogen Candidates in the Mouse Model of Brucellosis  

E-Print Network [OSTI]

and identification modeling approach provides strong evidence for prediction of Brucella protective antigens, and represent a novel strategy with broad application to other major pathogens....

Gomez, Gabriel

2013-02-26T23:59:59.000Z

436

Exposure to ornithine results in excessive accumulation of putrescine and apoptotic cell death in ornithine decarboxylase overproducing mouse myeloma cells  

Science Journals Connector (OSTI)

...Cell Growth & Differentiation 1283 5 R. Poulin arid A. E. Pegg, personal communication...Swartzendruber, D., and Pierce, G. B. 64. Poulin, R., Wechter, R. S., and Pegg...Chem., 266: 6142-6151, 1991. 51. Poulin, R., Coward, I. K., Lakanen, J...

KE Tobias and C Kahana

1995-10-01T23:59:59.000Z

437

Long-term epigenetic effects of exposure to low doses of 56Fe in the mouse lung  

Science Journals Connector (OSTI)

......The dose-effect was statistically assessed using ROUT's test (to eliminate outliers) and one-way ANOVA and Kruskal-Wallis methods, followed by Tukey's test and the Jonckheere trend test when appropriate. RESULTS Analysis of expression......

Etienne Nzabarushimana; Isabelle R. Miousse; Lijian Shao; Jianhui Chang; Antiño R. Allen; Jennifer Turner; Blair Stewart; Jacob Raber; Igor Koturbash

2014-07-01T23:59:59.000Z

438

Effect of Irradiation on Mouse Salivary Glands during the Prereplicative Phase of Isoproterenol-stimulated DNA Synthesis  

Science Journals Connector (OSTI)

...Toda Department of Radiology, Tokyo Medical and Dental University, School...Toda Department of Radiology, Tokyo Medical and Dental University, School...Microscopic Changes. J. Dental, Res.,47...X-irradiation. Radiology, 93: 307-313...

Takehito Sasaki and Masatoshi Toda

1972-12-01T23:59:59.000Z

439

Formation of Benzo(a)pyrene/DNA Adducts and Their Relationship to Tumor Initiation in Mouse Epidermis  

Science Journals Connector (OSTI)

...DiGiovanni,2 and Thomas J. Slaga Biology Division. Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 [S. W. A., T. J. S...BPDE/ DNA adduct in the treated cells. Other workers have shown that a correlation exists between formation...

Stephen W. Ashurst; Gerald M. Cohen; Stephen Nesnow; John DiGiovanni; and Thomas J. Slaga

1983-03-01T23:59:59.000Z

440

Glucose-6-phosphatase deficiency caused by radiation-induced alleles at the albino locus in the mouse  

Science Journals Connector (OSTI)

...C14COS) were kindly sent to us by Dr. L. B. Russell of Oak Ridge for a study of the nature of their lethal effects. An...1 jumoles/gm/min, respectively. Two groups of workers 2 3 have produced evidence that the microsomal glucose...

R P Erickson; S Gluecksohn-Waelsch; C F Cori

1968-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

The Effect of Roentgen Radiation on the Incorporation of Radiophosphorus into Nucleic Acids and Other Constituents of Mouse Mammary Carcinoma  

Science Journals Connector (OSTI)

...total-body radiation administered by other workers. Furthermore, 4,500 r causes...Na,HPO4 on allocation from the Oak Ridge National Laboratory. Trial experiments...radiation ad ministered by other workers. Furthermore, 4,500 r causes...

Halvor Vermund; Cyrus P. Barnum; Robert A. Huseby; and Karl W. Stenstrom

1953-09-01T23:59:59.000Z

442

Effects of the Over-Expression of Neuronal Glutamate Dehydrogenase (Glud1) on Mouse Brain Mitochondrial Function  

E-Print Network [OSTI]

Michaelis for the countless times she listened to my seminars and laboratory presentations and continued to provide support and feedback. The remaining members of my committee: Drs. Rick Dobrowsky; Robert Cohen, and Honglian Shi, thank you for all your...’s disease. Therefore, the Glud1 Tg mice may be used to probe the molecular and cellular pathways involved in selective neuronal vulnerability as it may relate to excess extracellular glutamate. There is not much information on this topic and several...

Anguiano, Laura

2012-08-31T23:59:59.000Z

443

Interaction of Carcinogenic Hydrocarbons with Tissues: VIII. Binding of Tritium-labeled Hydrocarbons to the Soluble Proteins of Mouse Skin  

Science Journals Connector (OSTI)

...treated as described in "Materials and Methods." A demonstration...of the unbound radio active hydrocarbon was removed...migrated toward the cathode, a neutral in dicator...pro teins toward the cathode was affected by electro...dermis as described in "Materials and Methods." The...

C. W. Abell and Charles Heidelberger

1962-09-01T23:59:59.000Z

444

Characterization of the Multidrug Resistance Protein Expressed in Cell Clones Stably Transfected with the Mouse mdr1 cDNA  

Science Journals Connector (OSTI)

...transfection of a biolog ically active cDNA presents distinct...out as described ("Materials and Methods"). The...positions of the anode and cathode are indicated . Arrow...second transcriptionally active member and have identified...third transcriptionally active mdr gene.1 The systematic...

Erwin Schurr; Martine Raymond; John C. Bell; and Philippe Gros

1989-05-15T23:59:59.000Z

445

Comparative 32P-Analysis of Cigarette Smoke-induced DNA Damage in Human Tissues and Mouse Skin  

Science Journals Connector (OSTI)

...5 Murea.pH3.64.2 M lithium formate, 7.5 Murea.pH3.80.8 M lithium chloride, 0.5 MTrisMCI, 8.5 M urea, pH8.00.6 M lithium chloride, 0.5 MTrisHCI...labeled quantitatively with excess ATP, and was calculated according...

Erika Randerath; Tommie A. Avitts; M. Vijayaraj Reddy; Robert H. Miller; Richard B. Everson; and Kurt Randerath

1986-11-01T23:59:59.000Z

446

Mechanism of Induction of c-fos by Ultraviolet B (290–320 nm) in Mouse JB6 Epidermal Cells  

Science Journals Connector (OSTI)

...portion of the solar spectrum possesses...cancer and has the capacity to stimulate epidermal...portion of the solar spectrum possesses...cancer and has the capacity to stimulate epidermal...portion of the solar spectrum possesses...cancer and has the capacity to stimulate epidermal...

Girish Shah; Rita Ghosh; Paul A. Amstad; and Peter A. Cerutti

1993-01-01T23:59:59.000Z

447

Effects of Chronic Low-Dose Ultraviolet B Radiation on DNA Damage and Repair in Mouse Skin  

Science Journals Connector (OSTI)

...of mammalian skin to solar UVB. We analyzed DNA...significantly less repair capacity than rapidly dividing...the loss of this repair capacity could partially contribute...in excision-repair capacity, and potentiation of...low-dose exposure to solar UVB may result in a significant...

David L. Mitchell; Rüdiger Greinert; Frank R. de Gruijl; Kees L. H. Guikers; Eckhard W. Breitbart; Michelle Byrom; Michelle M. Gallmeier; Megan G. Lowery; and Beate Volkmer

1999-06-15T23:59:59.000Z

448

A Mouse Model for the Carney Complex Tumor Syndrome Develops Neoplasia in Cyclic AMP–Responsive Tissues  

Science Journals Connector (OSTI)

...Lymphoproliferative disease 318 F 23.9 + + Bronchioloalveolar carcinoma, pit adenoma 290 F 24.3 + + + (Usual, atypical) Carcinoma 974...Genet 2000;9:3055-64. 45 Zhu Y, Ghosh P, Charnay P, Burns DK, Parada LF. Neurofibromas in NF1: Schwann cell origin and...

Lawrence S. Kirschner; Donna F. Kusewitt; Ludmila Matyakhina; William H. Towns II; J. Aidan Carney; Heiner Westphal; and Constantine A. Stratakis

2005-06-01T23:59:59.000Z

449

Identification and characterisation of glucagon-like peptide-1 receptor expressing cells using a new transgenic mouse model  

E-Print Network [OSTI]

coverslipped and viewed using epifluorescence (Nikon Eclipse 80; Kingston upon Thames, UK). Photomicrographs were taken with a Micropublisher 3.3 RTV camera and QCapture Pro software (Qimaging Inc., Surrey, BC, Canada). Nodose and Enteric Ganglia culture Nodose... -fluorescence was prominent in the area postrema. This is a region with a leaky blood brain barrier that has been postulated previously as an area potentially capable of responding to circulating GLP-1 concentrations (24). In the hypothalamus, we found glp1r...

Richards, Paul; Parker, Helen E.; Adriaenssens, Alice E.; Hodgson, Joshua M.; Cork, Simon C.; Trapp, Stefan; Gribble, Fiona M.; Reimann, Frank

2013-12-02T23:59:59.000Z

450

Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Does Not Improve Paclitaxel Effect in an Orthotopic Mouse Model of Lung Cancer  

Science Journals Connector (OSTI)

...analyses were prepared in 10 mmol/L ethylene diamine tetraacetic acid buffer (pH 8...2003 1200-10. 17 Weber KL, Doucet M, Price JE, Baker C, Kim SJ, Fidler IJ Blockade...Yoneda J, Kuniyasu H, Crispens MA, Price JE, Bucana CD, Fidler IJ Expression...

Amir Onn; Takeshi Isobe; Wenjuan Wu; Satoshi Itasaka; Tomoaki Shintani; Keiko Shibuya; Yokoi Kenji; Michael S. O’Reilly; Isaiah J. Fidler; and Roy S. Herbst

2004-12-15T23:59:59.000Z

451

UV radiation facilitates methotrexate resistance and amplification of the dihydrofolate reductase gene in cultured 3T6 mouse cells.  

Science Journals Connector (OSTI)

...UV pretreatment; UV radiation, 3T6 clone 5 colonies...pretreatment with 3 J of UV radiation per m2. z w 5...12 h. A 4-J/m2 dose of UV radiation was given, and drug-free...shown on a log scale. estimation of the number of MTX-resistant...

T D Tlsty; P C Brown; R T Schimke

1984-06-01T23:59:59.000Z

452

Dietary Fish Oil Alters T Lymphocyte Cell Populations and Exacerbates Disease in a Mouse Model of Inflammatory Colitis  

Science Journals Connector (OSTI)

...Caryocar brasiliense Camb) fruit pulp oil decrease the DNA damage caused by chemical...flavonoids, saponins and tannins, and essential oils. The aim of this study was to evaluate the action of the pequi oil in the DNA damage in experimental model...

Hillary L. Woodworth; Sarah J. McCaskey; David M. Duriancik; Jonathan F. Clinthorne; Ingeborg M. Langohr; Elizabeth M. Gardner; Jenifer I. Fenton

2010-10-15T23:59:59.000Z

453

PET Imaging of Tumor Neovascularization in a Transgenic Mouse Model with a Novel 64Cu-DOTA-Knottin Peptide  

Science Journals Connector (OSTI)

...Choice and Handling of Clinical...asked and technology to be used...processing procedures exist for...can also be applied to the TaqMan...spectrometry. The technology is based...analysis. This procedure can be applied to the discovery...methylation. The procedure is very rapid...many other technologies for methylation...

Carsten H. Nielsen; Richard H. Kimura; Nadia Withofs; Phuoc T. Tran; Zheng Miao; Jennifer R. Cochran; Zhen Cheng; Dean Felsher; Andreas Kjær; Juergen K. Willmann; and Sanjiv S. Gambhir

2010-11-15T23:59:59.000Z

454

Brain function and macromolecules. 3. Uridine incorporation into polysomes of mouse brain during short-term avoidance conditioning  

Science Journals Connector (OSTI)

...density gradient penrofuaie of Polysomes bfore andcaftermincubation wit olsacrfied n hris were quckyreoedlllprcdueewr as described in...1963), vol. 1, p. 76. lo Tsuboi, K. K., and T. D. Price, Arch. Biochem. Biophys., 81, 223 (1959). 16 Randerath...

L B Adair; J E Wilson; J W Zemp; E Glassman

1968-01-01T23:59:59.000Z

455

An Xpb Mouse Model for Combined Xeroderma Pigmentosum and Cockayne Syndrome Reveals Progeroid Features upon Further Attenuation of DNA Repair  

Science Journals Connector (OSTI)

...J. O., J. Jans, J. de Wit, F. Coin, D. Hoogstraten...J. R. Mitchell, J. de Wit, D. Hoogstraten, M. Volker...Stefanini, E. Menefee, V. H. Price, S. Queille, A. Sarasin...J. O. Andressoo, J. de Wit, J. Huijmans, R. B. Beems...

Jaan-Olle Andressoo; Geert Weeda; Jan de Wit; James R. Mitchell; Rudolf B. Beems; Harry van Steeg; Gijsbertus T. J. van der Horst; Jan H. Hoeijmakers

2008-12-29T23:59:59.000Z

456

Proximal Fluid Proteome Profiling of Mouse Colon Tumors Reveals Biomarkers for Early Diagnosis of Human Colorectal Cancer  

Science Journals Connector (OSTI)

...Human Colorectal Cancer Remond J.A. Fijneman 1 2 Meike de Wit 1 Maral Pourghiasian 1 2 Sander R. Piersma 2 Thang V. Pham...1065-82. 33. Johansen JS , Jensen BV, Roslind A, Nielsen D Price PA.Serum YKL-40, a new prognostic biomarker in cancer patients...

Remond J.A. Fijneman; Meike de Wit; Maral Pourghiasian; Sander R. Piersma; Thang V. Pham; Marc O. Warmoes; Mehrdad Lavaei; Chloe Piso; Fiona Smit; Pien M. Delis-van Diemen; Sietze T. van Turenhout; Jochim S. Terhaar sive Droste; Chris J.J. Mulder; Marinus A. Blankenstein; Els C. Robanus-Maandag; Ron Smits; Riccardo Fodde; Victor W.M. van Hinsbergh; Gerrit A. Meijer; and Connie R. Jimenez

2012-05-01T23:59:59.000Z

457

A Hexane Fraction of American Ginseng Suppresses Mouse Colitis and Associated Colon Cancer: Anti-inflammatory and Proapoptotic Mechanisms  

Science Journals Connector (OSTI)

...inducible nitric oxide synthase to a basal-like...protein kinase C-epsilon-Raf-1-MEK-1...transcription 1 and 3 and induction of...inducible nitric oxide synthase promoter by the iron chelator desferrioxamine...inducible nitric-oxide synthase promoter...Rev Cancer 2003;3:276-85. 44...

Deepak Poudyal; Phuong Mai Le; Tia Davis; Anne B. Hofseth; Alena Chumanevich; Alexander A. Chumanevich; Michael J. Wargovich; Mitzi Nagarkatti; Prakash S. Nagarkatti; Anthony Windust; and Lorne J. Hofseth

2012-04-01T23:59:59.000Z

458

Analysis of Cell Cycle in Mouse Bone Marrow Cells Following Acute in vivo Exposure to 56Fe Ions  

Science Journals Connector (OSTI)

......Stony Brook University Stony Brook, NY 11794-8691 2 Molecular Epidemiology Program University of Texas Medical Branch at Galveston Galveston, TX 77555-1153 A pilot study was conducted to examine the magnitude of cell-cycle delay and apoptosis......

Kanokporn Noy Rithidech; Marc Golightly; Elbert Whorton

2008-07-01T23:59:59.000Z

459

DNA repair efficiency in germ cells and early mouse embryos and consequences for radiation-induced transgenerational genomic damage  

E-Print Network [OSTI]

mutation rate in the post-chernobyl families from ukraine.mutation rate after the Chernobyl accident. Nature 380, 683-fallout following the Chernobyl accident (Dubrova et al. ,

Marchetti, Francesco

2009-01-01T23:59:59.000Z

460

Evaluation of Toxicity following Electrically Mediated Interleukin-12 Gene Delivery in a B16 Mouse Melanoma Model  

Science Journals Connector (OSTI)

...Pottinger C, Jaroszeski MJ, Gilbert R, Heller R. In vivo electroporation...LC, Ingram SF, Lucas ML, Gilbert RA, Heller R. Effect of...2336-42. 35 Heller R, Gilbert R, Jaroszeski MJ. Clinical...Department of Medical Microbiology and Immunology, University...

Loree Heller; Kathleen Merkler; Jeffrey Westover; Yolmari Cruz; Domenico Coppola; Kaaron Benson; Adil Daud; and Richard Heller

2006-05-15T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman  

E-Print Network [OSTI]

major depression," Psychiatry Res. 151, 179-188 (2007). 3. S. J. Wright, V. E. Centonze, S. A. Stricker. Dolle, C. Wu, D. Feinstein, G.-F. Huang, F. Semah, C. A. Mathis, W. Klunk, R. M. Gould, C. Lubetzki, 9304-9309 (2006). 5. A. Sherbondy, D. Akers, R. Mackenzie, R. Dougherty, and B. Wandell, "Exploring

Cheng, Ji-Xin

462

Modulation of the Cell Cycle of Cultured Mouse Liver Cells by Benzo(a)pyrene and Its Derivatives  

Science Journals Connector (OSTI)

...FCM DNA histograms and the doubling times (2) and assumes that all cells are cycling. CMean S.D. (Diol-Epoxide)-(DMSC(Diol-E@oxide)-VMS0) 0 @ -I0 0 poration into the DNA during 15-mm pulses to logarithmically growing cultures. Chart...

James C. Bartholomew; Andrew L. Pearlman; Joseph R. Landolph; Kenneth Straub

1979-07-01T23:59:59.000Z

463

Modulation of the Cell Cycle of Cultured Mouse Liver Cells by Benzo(a)pyrene and Its Derivatives  

Science Journals Connector (OSTI)

...this data analysis technique was within experimental error of standard autoradiography techniques for estimating the proportion of...all cells are cycling. CMean S.D. (Diol-Epoxide)-(DMSC(Diol-E@oxide)-VMS0) 0 @ -I0 0 poration into the DNA...

James C. Bartholomew; Andrew L. Pearlman; Joseph R. Landolph; and Kenneth Straub

1979-07-01T23:59:59.000Z

464

Abstract 1561: An evaluation of the use of genetically modified mouse models in human cancer risk assessment.  

Science Journals Connector (OSTI)

...and educating the public about acceptable risk profiles for given chemopreventive...presence or number of cancers and an acceptable risk-benefit ratio have been the...precancers, however, may, with an acceptable risk-benefit ratio, also lead to...

David A. Eastmond; Suryanarayana V. Vulimiri; John E. French; and Babasaheb Sonawane

2013-08-14T23:59:59.000Z

465

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences  

Science Journals Connector (OSTI)

...Sequencing. Several different strategies were used for full-insert cDNA sequencing, including primer walking (http://www-shgc.stanford.edu/Seq/cdnapages/maincdna.html), transposon insertions (8, 9), and concatenated shotgun sequencing...

Mammalian Gene Collection (MGC) Program Team

2002-01-01T23:59:59.000Z

466

RAPID 3D TRACING OF THE MOUSE BRAIN NEUROVASCULATURE WITH LOCAL MAXIMUM INTENSITY PROJECTION AND MOVING WINDOWS  

E-Print Network [OSTI]

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 1. 3D Frangi vs. 2D Frangi with MIP cube . . . . . . . . 49 a. Synthetic data tracing result . . . . . . . . . . . . 49 b. Performance comparison . . . . . . . . . . . . . . 50 c. Validation . . . . . . . . . . . . . . . . . . . . . . 51 2. MW... with MIP cube . . . . . . . . . . . . . . . . . . . 53 a. Synthetic data tracing result . . . . . . . . . . . . 53 b. Performance comparison . . . . . . . . . . . . . . 54 c. Validation . . . . . . . . . . . . . . . . . . . . . . 54 3. Tracing in low...

Han, Dong Hyeop

2011-10-21T23:59:59.000Z

467

Mouse model of endemic Burkitt translocations reveals the long-range boundaries of Ig-mediated oncogene deregulation  

Science Journals Connector (OSTI)

...plasma cell neoplasms . DNA Repair (Amst) 5 : 1213 – 1224 . 45 Gostissa...Analyzer and deep-sequencing pipeline; Val Bliskovsky for assistance...Translocations. Payer's patches, mesenteric lymph node, and...and corresponding qualities. Pipeline out-put...

Alexander L. Kovalchuk; Camilo Ansarah-Sobrinho; Ofir Hakim; Wolfgang Resch; Helena Tolarová; Wendy Dubois; Arito Yamane; Makiko Takizawa; Isaac Klein; Gordon L. Hager; Herbert C. Morse III; Michael Potter; Michel C. Nussenzweig; Rafael Casellas

2012-01-01T23:59:59.000Z

468

Structures of the DNA Adducts Formed in Mouse Liver after Administration of the Proximate Hepatocarcinogen 1?-Hydroxyestragole  

Science Journals Connector (OSTI)

...3-3H]hydroxyestragole. Reaction of 1-acetoxyestragole, a...coeluted with a product of the reaction of 1-acetoxyestragole with...mechanisms (Chart 12). In a reaction mechanism of predominantly...Alternatively, a predominantly SN2 mechanism would yield Adduct...

David H. Phillips; James A. Miller; Elizabeth C. Miller; and Bruce Adams

1981-01-01T23:59:59.000Z

469

Carcinogenicity of benzo[a]pyrene 4,5-, 7,8-, and 9,10-oxides on mouse skin  

Science Journals Connector (OSTI)

...NIAMDD, NIH, Bethesda, Maryland 20014 Communicated by J. J. Burns, October 14, 1975 ABSTRACT Benzo[alpyrene and three arene...23. Grover, P. L., Sims, P., Mitchley, B. C. V. & Roe, F. J. C. (1975) "The carcinogenicity of polycyclic hydrocarbon...

W Levin; A W Wood; H Yagi; P M Dansette; D M Jerina; A H Conney

1976-01-01T23:59:59.000Z

470

v-Ha-ras transgene abrogates the initiation step in mouse skin tumorigenesis: effects of phorbol esters and retinoic acid.  

Science Journals Connector (OSTI)

...K. (1974) CRC Crit. Rev. Toxicol. 2, 419-431. 3. Burns, F. J., Vanderlaan, M., Snyder, E. & Albert, R. E...and Cancer: From Basic Research to Policy Implications, ed. Roe, D. (Liss, New York), pp. 95-109. 34. Lichti, U...

A Leder; A Kuo; R D Cardiff; E Sinn; P Leder

1990-01-01T23:59:59.000Z

471

Parathyroid Gland-specific Deletion of the Mouse Men1 Gene Results in Parathyroid Neoplasia and Hypercalcemic Hyperparathyroidism  

Science Journals Connector (OSTI)

...S. Y., Spiegel A. M., Burns A. L., Debelenko L. V...Crabtree J. S., Wang Y., Roe B. A., Weisemann J., Boguski...Dong Q., Spiegel A. M., Burns A. L., Marx S. J. Positional...F. S., Spiegel A. M., Burns A. L., Marx S. J. Somatic...

Steven K. Libutti; Judy S. Crabtree; Dominique Lorang; A. Lee Burns; Chiara Mazzanti; Stephen M. Hewitt; Sarah O’Connor; Jerrold M. Ward; Michael R. Emmert-Buck; Alan Remaley; Marshall Miller; Ewa Turner; H. Richard Alexander; Andrew Arnold; Stephen J. Marx; Francis S. Collins; and Allen M. Spiegel

2003-11-15T23:59:59.000Z

472

Effects of Type of Dietary Fat on Phorbol Ester-elicited Tumor Promotion and Other Events in Mouse Skin  

Science Journals Connector (OSTI)

...PROMOTION carcinoma development; the second part...CLA inhibited the development of tumors in the...standards, and snake venom phospholipase...The column oven temperature was programmed...by sonication. Snake venom phospholipase...incubation at room temperature for 30 min. At...

Julius Leyton; Marilyn L. Lee; Mary Locniskar; Martha A. Belury; Thomas J. Slaga; David Bechtel; and Susan M. Fischer

1991-02-01T23:59:59.000Z

473

Lack of Carcinogenicity of 4-, 5-, 6-, 7-, 8-, 9-, and 10-Hydroxybenzo(a)pyrene on Mouse Skin  

Science Journals Connector (OSTI)

...weight. The dorsal region of the animals was shaved with electric clippers 24 hr before the test compounds were applied. The...polycyclic aromatic hydrocarbon BP2 in polluted city air, automobile exhaust, cigarette smoke, and elsewhere in man's environment...

Jaime Kapitulnik; Wayne Levin; Haruhiko Yagi; Donald M. Jerina; and Allan H. Conney

1976-10-01T23:59:59.000Z

474

Divergent neuroactive steroid responses to stress and ethanol in rat and mouse strains: relevance for human studies  

Science Journals Connector (OSTI)

This review will summarize the pharmacological properties and physiological regulation of neuroactive steroids, with a particular focus on divergent neuroactive steroid responses to stress and ethanol in rats, mi...

Patrizia Porcu; A. Leslie Morrow

2014-09-01T23:59:59.000Z

475

Chronic cisplatin treatment promotes enhanced damage repair and tumor progression in a mouse model of lung cancer  

E-Print Network [OSTI]

Chemotherapy resistance is a major obstacle in cancer treatment, yet the mechanisms of response to specific therapies have been largely unexplored in vivo. Employing genetic, genomic, and imaging approaches, we examined ...

Oliver, Trudy Gale

476

Abstract 62: Third hit genetic changes and clonal heterogeneity in a genetically engineered mouse lung cancer model  

Science Journals Connector (OSTI)

...markers, and colony forming efficiency (CFE) and colony morphology in 3D culture...growth rates, lineage marker staining, CFE, and 3D colony morphology. All 4 clones...rates, lineage marker expression patterns, CFE, 3D cell morphology and variable in vivo...

Sang-Won Um; Ping-Jie Xiao; David Neil Hayes; William Y. Kim; Scott H. Randell

2014-10-01T23:59:59.000Z

477

FGF-2 promotes neurogenesis and neuroprotection and prolongs survival in a transgenic mouse model of Huntington's disease  

Science Journals Connector (OSTI)

...protein aggregate formation, restoration toward normal of CB1 cannabinoid...from their actions in other systems, but in other cases are unclear...2003 ) in The Cytokine Handbook , eds. Thomson, A. W. & Lotze, M...pH 6.0, for 2 min at 40% power in a 1,100-W microwave...

Kunlin Jin; Michelle LaFevre-Bernt; Yunjuan Sun; Sylvia Chen; Juliette Gafni; Danielle Crippen; Anna Logvinova; Christopher A. Ross; David A. Greenberg; Lisa M. Ellerby

2005-01-01T23:59:59.000Z

478

A Gnotobiotic Mouse Model Demonstrates That Dietary Fiber Protects against Colorectal Tumorigenesis in a Microbiota- and Butyrate-Dependent Manner  

Science Journals Connector (OSTI)

...muL of either 2 TaqMan or 2 Power SYBR Green Master Mix (Applied Biosystems) with...accumulation was monitored by FAM or SYBR Green fluorescence. Control reactions lacking...procedures. qPCR was performed using Power SYBR Green Master Mix (Applied Biosystems) on an...

Dallas R. Donohoe; Darcy Holley; Leonard B. Collins; Stephanie A. Montgomery; Alan C. Whitmore; Andrew Hillhouse; Kaitlin P. Curry; Sarah W. Renner; Alicia Greenwalt; Elizabeth P. Ryan; Virginia Godfrey; Mark T. Heise; Deborah S. Threadgill; Anna Han; James A. Swenberg; David W. Threadgill; and Scott J. Bultman

2014-12-01T23:59:59.000Z

479

An integrative functional genomics approach towards quantitative trait gene nomination in existing and emerging mouse genetic reference populations.  

E-Print Network [OSTI]

??An approach that has been widely applied for the genetic dissection of complex traits is Quantitative Trait Locus (QTL) mapping. QTL mapping identifies genomic regions… (more)

Philip, Vivek

2012-01-01T23:59:59.000Z

480

Abstract A32: Studying circadian disruption as a novel risk factor of hepatocellular carcinoma using mouse models  

Science Journals Connector (OSTI)

...Genome-wide array analyses have led to identify a time-dependent deregulation of all known key pathways controlling metabolism, energy storage, redox levels, cell proliferation, inflammatory response and tumor suppression in the liver of mice lacking circadian...

Nicole M. Kettner; David D. Moore; Loning Fu

2014-11-01T23:59:59.000Z

Note: This page contains sample records for the topic "mouse mus musculus" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Effects of Fuel Ignition Quality on Critical Equivalence Ratio for Autoignition  

Science Journals Connector (OSTI)

This hypothesis is based on the work of Musculus et al.,(2) who introduced the concept of a “critical” equivalence ratio (?), below which fuel in localized regions of the chamber is a potential source for incomplete combustion. ... The evaporative steam cooling system, used to maintain water jacket temperatures of the engine in the standard CFR engine design,(12) was replaced with an 8 L 1000 W refrigerated/heating circulator. ... Musculus et al.,(2) who first presented the concept of critical ?, stated that “UHC emissions may arise from jets that ignite before EOI (negative ignition dwell) if the mixtures near the injector become too lean either to autoignite in the time available or to support propagation of the downstream reaction zone into the near-injector region.” ...

Gregory K. Lilik; André L. Boehman

2013-02-11T23:59:59.000Z

482

Process for recovery of hydrogen and  

DOE Patents [OSTI]

on of sulfur Abstract A process of abstracting sulfur from H.sub.2 S and generating hydrogen is disclosed comprising dissolving Pd.sub.2 X.sub.2 (.mu.-dppm).sub.2 in a solvent and then introducing H.sub.2 S. The palladium complex abstracts sulfur, forming hydrogen and a (.mu.-S) complex. The (.mu.-S) complex is readily oxidizable to a (.mu.-SO.sub.2) adduct which spontaneously loses SO.sub.2 and regenerates the palladium complex.

James, Brian R. (Vancouver, CA); Li-Lee, Chung (Vancouver, CA); Lilga, Michael A. (Richland, WA); Nelson, David A. (Richland, WA)

1987-01-01T23:59:59.000Z

483

Engineering shallow spins in diamond with nitrogen delta-doping  

SciTech Connect (OSTI)

We demonstrate nanometer-precision depth control of nitrogen-vacancy (NV) center creation near the surface of synthetic diamond using an in situ nitrogen delta-doping technique during plasma-enhanced chemical vapor deposition. Despite their proximity to the surface, doped NV centers with depths (d) ranging from 5 to 100 nm display long spin coherence times, T{sub 2} > 100 {mu}s at d = 5 nm and T{sub 2} > 600 {mu}s at d {>=} 50 nm. The consistently long spin coherence observed in such shallow NV centers enables applications such as atomic-scale external spin sensing and hybrid quantum architectures.

Ohno, Kenichi; Joseph Heremans, F.; Bassett, Lee C.; Myers, Bryan A.; Toyli, David M.; Bleszynski Jayich, Ania C.; Palmstrom, Christopher J.; Awschalom, David D. [Center for Spintronics and Quantum Computation, University of California, Santa Barbara, California 93106 (United States)

2012-08-20T23:59:59.000Z

484

Leptospirosis in striped skunks  

E-Print Network [OSTI]

, Poi L. coxus, Cox L. canicole, Hond Utrecht IV L, schueffneri, Vleermuis 90 C L. b~1, B L, ~onsis~ Jones L. sumneriv Sumner L, m~ala a, H-6 L. ~atane~ Patane L. bellum (A), Mus 127 L. baU. um (AB), Castellon 3 L. ~&~o teri, 3522 C L..., Poi L. coxus, Cox L. canicole, Hond Utrecht IV L, schueffneri, Vleermuis 90 C L. b~1, B L, ~onsis~ Jones L. sumneriv Sumner L, m~ala a, H-6 L. ~atane~ Patane L. bellum (A), Mus 127 L. baU. um (AB), Castellon 3 L. ~&~o teri, 3522 C L...

Roth, Earl Eugene

2012-06-07T23:59:59.000Z

485

Dual spatial maps of transcript and protein abundance in the...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Dual spatial maps of transcript and protein abundance in the mouse brain. Dual spatial maps of transcript and protein abundance in the mouse brain. Abstract: Integrating...

486

Peripheral neuropathy caused by DCA-induced oxidative stress  

E-Print Network [OSTI]

39 Figure 3: Control + fisetin + DHA mouse study40 Figure 4: DCA + fisetin + DHA mouse studyby the dietary compound fisetin. Am J Physiol Gastrointest

Liou, Charlie

2011-01-01T23:59:59.000Z

487

E-Print Network 3.0 - accurate genome alignment Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

; Biology and Medicine 3 Automated Whole-Genome Multiple Alignment of Rat, Mouse, and Human Summary: Automated Whole-Genome Multiple Alignment of Rat, Mouse, and Human Michael...

488

E-Print Network 3.0 - arabinoside-metabolizing enzyme genes Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

6 Animal Models of Gaucher Disease Transgenic mouse lines engineered to express the human GBA enzyme Summary: Animal Models of Gaucher Disease Transgenic mouse lines...

489

The effects of physiological age on bone marrow-derived mesenchymal stem cells  

E-Print Network [OSTI]

Jaenisch. Treatment of sickle cell anemia mouse model withcan be used to treat sickle cell anemia in a mouse model 5 ,

Buckspan, Caitlin

2010-01-01T23:59:59.000Z

490

C3Bio.org - Members: View: Robert C. Brown  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Suitcase Mouse Penguin Teapot Chicken Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

491

C3Bio.org - Members: View: Shi-You Ding  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Mouse Suitcase Chicken Teapot Penguin Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

492

C3Bio.org - Tags: Sample: Plant Material  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Mouse Penguin Chicken Suitcase Teapot Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

493

C3Bio.org - Questions and Answers: Question: How can I get involved...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Mouse Penguin Chicken Suitcase Teapot Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

494

C3Bio.org - Resources: Keynote - How Shall We Choose? Making...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Chicken Teapot Suitcase Penguin Mouse Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

495

C3Bio.org - Resources: The Plant Actin Cytoskeleton Responds...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Suitcase Mouse Penguin Teapot Chicken Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

496

C3Bio.org - Resources: Quantum Dot Lab  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Mouse Suitcase Chicken Teapot Penguin Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

497

C3Bio.org - Resources: Envisioning the transition to a next-generation...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Mickey Mouse is a... - Select an answer - Mouse Penguin Chicken Suitcase Teapot Please leave this field blank. required Problem: required Attach a screenshot: optional (.jpg,...

498

C3Bio.org - Wiki: hough replays demonstrated there was no contact...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

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499

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