National Library of Energy BETA

Sample records for moist mineral-matter-free basis

  1. Moist caustic leaching of coal

    DOE Patents [OSTI]

    Nowak, Michael A.

    1994-01-01

    A process for reducing the sulfur and ash content of coal. Particulate coal is introduced into a closed heated reaction chamber having an inert atmosphere to which is added 50 mole percent NaOH and 50 mole percent KOH moist caustic having a water content in the range of from about 15% by weight to about 35% by weight and in a caustic to coal weight ratio of about 5 to 1. The coal and moist caustic are kept at a temperature of about 300.degree. C. Then, water is added to the coal and caustic mixture to form an aqueous slurry, which is washed with water to remove caustic from the coal and to produce an aqueous caustic solution. Water is evaporated from the aqueous caustic solution until the water is in the range of from about 15% by weight to about 35% by weight and is reintroduced to the closed reaction chamber. Sufficient acid is added to the washed coal slurry to neutralize any remaining caustic present on the coal, which is thereafter dried to produce desulfurized coal having not less than about 90% by weight of the sulfur present in the coal feed removed and having an ash content of less than about 2% by weight.

  2. Multiscale Simulation of Moist Global Atmospheric Flows

    SciTech Connect (OSTI)

    Grabowski, Wojciech W.; Smolarkiewicz, P. K.

    2015-04-13

    The overarching goal of this award was to include phase changes of the water substance and accompanying latent heating and precipitation processes into the all-scale nonhydrostatic atmospheric dynamics EUlerian/LAGrangian (EULAG) model. The model includes fluid flow solver that is based on either an unabbreviated set of the governing equations (i.e., compressible dynamics) or a simplified set of equations without sound waves (i.e., sound-proof, either anelastic or pseudo-incompressible). The latter set has been used in small-scale dynamics for decades, but its application to the all-scale dynamics (from small-scale to planetary) has never been studied in practical implementations. The highlight of the project is the development of the moist implicit compressible model that can be run by applying time steps, as long as the anelastic model is limited only by the computational stability of the fluid flow and not by the speed of sound waves that limit the stability of explicit compressible models. Applying various versions of the EULAG model within the same numerical framework allows for an unprecedented comparison of solutions obtained with various sets of the governing equations and straightforward evaluation of the impact of various physical parameterizations on the model solutions. The main outcomes of this study are reported in three papers, two published and one currently under review. These papers include comparisons between model solutions for idealized moist problems across the range of scales from small to planetary. These tests include: moist thermals rising in the stable-stratified environment (following Grabowski and Clark, J. Atmos. Sci. 1991) and in the moist-neutral environment (after Bryan and Fritsch, Mon. Wea. Rev. 2002), moist flows over a mesoscale topography (as in Grabowski and Smolarkiewicz, Mon. Wea. Rev. 2002), deep convection in a sheared environment (following Weisman and Klemp, Mon. Wea. Rev. 1982), moist extension of the baroclinic wave on

  3. Moist multi-scale models for the hurricane embryo

    SciTech Connect (OSTI)

    Majda, Andrew J.; Xing, Yulong; Mohammadian, Majid

    2010-01-01

    Determining the finite-amplitude preconditioned states in the hurricane embryo, which lead to tropical cyclogenesis, is a central issue in contemporary meteorology. In the embryo there is competition between different preconditioning mechanisms involving hydrodynamics and moist thermodynamics, which can lead to cyclogenesis. Here systematic asymptotic methods from applied mathematics are utilized to develop new simplified moist multi-scale models starting from the moist anelastic equations. Three interesting multi-scale models emerge in the analysis. The balanced mesoscale vortex (BMV) dynamics and the microscale balanced hot tower (BHT) dynamics involve simplified balanced equations without gravity waves for vertical vorticity amplification due to moist heat sources and incorporate nonlinear advective fluxes across scales. The BMV model is the central one for tropical cyclogenesis in the embryo. The moist mesoscale wave (MMW) dynamics involves simplified equations for mesoscale moisture fluctuations, as well as linear hydrostatic waves driven by heat sources from moisture and eddy flux divergences. A simplified cloud physics model for deep convection is introduced here and used to study moist axisymmetric plumes in the BHT model. A simple application in periodic geometry involving the effects of mesoscale vertical shear and moist microscale hot towers on vortex amplification is developed here to illustrate features of the coupled multi-scale models. These results illustrate the use of these models in isolating key mechanisms in the embryo in a simplified content.

  4. Laminar flame speeds of moist syngas mixtures

    SciTech Connect (OSTI)

    Das, Apurba K. [Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, OH 44106 (United States); Kumar, Kamal; Sung, Chih-Jen [Department of Mechanical Engineering, University of Connecticut, Storrs, CT 06269 (United States)

    2011-02-15

    This work experimentally investigates the effect of the presence of water vapor on the laminar flame speeds of moist syngas/air mixtures using the counterflow twin-flame configuration. The experimental results presented here are for fuel lean syngas mixtures with molar percentage of hydrogen in the hydrogen and carbon monoxide mixture varying from 5% to 100%, for an unburned mixture temperature of 323 K, and under atmospheric pressure. At a given equivalence ratio, the effect of varying amount of water vapor addition on the measured laminar flame speed is demonstrated. The experimental laminar flame speeds are also compared with computed values using chemical kinetic mechanisms reported in the literature. It is found that laminar flame speed varies non-monotonically with addition of water for the carbon monoxide rich mixtures. It first increases with increasing amount of water addition, reaches a maximum value, and then decreases. An integrated reaction path analysis is further conducted to understand the controlling mechanism responsible for the non-monotonic variation in laminar flame speed due to water addition. On the other hand, for higher values of H{sub 2}/CO ratio the laminar flame speed monotonically decreases with increasing water addition. It is shown that the competition between the chemical and thermal effects of water addition leads to the observed response. Furthermore, reaction rate sensitivity analysis as well as binary diffusion coefficient sensitivity analysis are conducted to identify the possible sources of discrepancy between the experimental and predicted values. The sensitivity results indicate that the reaction rate constant of H{sub 2}+OH = H{sub 2}O+H is worth revisiting and refinement of binary diffusion coefficient data of N{sub 2}-H{sub 2}O, N{sub 2}-H{sub 2}, and H{sub 2}-H{sub 2}O pairs can be considered. (author)

  5. A moist aquaplanet variant of the HeldSuarez test for atmospheric model dynamical cores

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Thatcher, D. R.; Jablonowski, C.

    2015-09-29

    A moist idealized test case (MITC) for atmospheric model dynamical cores is presented. The MITC is based on the HeldSuarez (HS) test that was developed for dry simulations on a flat Earth and replaces the full physical parameterization package with a Newtonian temperature relaxation and Rayleigh damping of the low-level winds. This new variant of the HS test includes moisture and thereby sheds light on the non-linear dynamics-physics moisture feedbacks without the complexity of full physics parameterization packages. In particular, it adds simplified moist processes to the HS forcing to model large-scale condensation, boundary layer mixing, and the exchange ofmorelatent and sensible heat between the atmospheric surface and an ocean-covered planet. Using a variety of dynamical cores of NCAR's Community Atmosphere Model (CAM), this paper demonstrates that the inclusion of the moist idealized physics package leads to climatic states that closely resemble aquaplanet simulations with complex physical parameterizations. This establishes that the MITC approach generates reasonable atmospheric circulations and can be used for a broad range of scientific investigations. This paper provides examples of two application areas. First, the test case reveals the characteristics of the physics-dynamics coupling technique and reproduces coupling issues seen in full-physics simulations. In particular, it is shown that sudden adjustments of the prognostic fields due to moist physics tendencies can trigger undesirable large-scale gravity waves, which can be remedied by a more gradual application of the physical forcing. Second, the moist idealized test case can be used to intercompare dynamical cores. These examples demonstrate the versatility of the MITC approach and suggestions are made for further application areas. The new moist variant of the HS test can be considered a test case of intermediate complexity.less

  6. moist aquaplanet variant of the Held–Suarez test for atmospheric model dynamical cores

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Thatcher, Diana R.; Jablonowski, Christiane

    2016-04-04

    moist idealized test case (MITC) for atmospheric model dynamical cores is presented. The MITC is based on the Held–Suarez (HS) test that was developed for dry simulations on “a flat Earth” and replaces the full physical parameterization package with a Newtonian temperature relaxation and Rayleigh damping of the low-level winds. This new variant of the HS test includes moisture and thereby sheds light on the nonlinear dynamics–physics moisture feedbacks without the complexity of full-physics parameterization packages. In particular, it adds simplified moist processes to the HS forcing to model large-scale condensation, boundary-layer mixing, and the exchange of latent and sensible heat betweenmore » the atmospheric surface and an ocean-covered planet. Using a variety of dynamical cores of the National Center for Atmospheric Research (NCAR)'s Community Atmosphere Model (CAM), this paper demonstrates that the inclusion of the moist idealized physics package leads to climatic states that closely resemble aquaplanet simulations with complex physical parameterizations. This establishes that the MITC approach generates reasonable atmospheric circulations and can be used for a broad range of scientific investigations. This paper provides examples of two application areas. First, the test case reveals the characteristics of the physics–dynamics coupling technique and reproduces coupling issues seen in full-physics simulations. In particular, it is shown that sudden adjustments of the prognostic fields due to moist physics tendencies can trigger undesirable large-scale gravity waves, which can be remedied by a more gradual application of the physical forcing. Second, the moist idealized test case can be used to intercompare dynamical cores. These examples demonstrate the versatility of the MITC approach and suggestions are made for further application areas. The new moist variant of the HS test can be considered a test case of intermediate complexity.« less

  7. moist aquaplanet variant of the Held–Suarez test for atmospheric model dynamical cores

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Thatcher, Diana R.; Jablonowski, Christiane

    2016-04-04

    moist idealized test case (MITC) for atmospheric model dynamical cores is presented. The MITC is based on the Held–Suarez (HS) test that was developed for dry simulations on “a flat Earth” and replaces the full physical parameterization package with a Newtonian temperature relaxation and Rayleigh damping of the low-level winds. This new variant of the HS test includes moisture and thereby sheds light on the nonlinear dynamics–physics moisture feedbacks without the complexity of full-physics parameterization packages. In particular, it adds simplified moist processes to the HS forcing to model large-scale condensation, boundary-layer mixing, and the exchange of latent and sensible heat betweenmore » the atmospheric surface and an ocean-covered planet. Using a variety of dynamical cores of the National Center for Atmospheric Research (NCAR)'s Community Atmosphere Model (CAM), this paper demonstrates that the inclusion of the moist idealized physics package leads to climatic states that closely resemble aquaplanet simulations with complex physical parameterizations. This establishes that the MITC approach generates reasonable atmospheric circulations and can be used for a broad range of scientific investigations. This paper provides examples of two application areas. First, the test case reveals the characteristics of the physics–dynamics coupling technique and reproduces coupling issues seen in full-physics simulations. In particular, it is shown that sudden adjustments of the prognostic fields due to moist physics tendencies can trigger undesirable large-scale gravity waves, which can be remedied by a more gradual application of the physical forcing. Second, the moist idealized test case can be used to intercompare dynamical cores. These examples demonstrate the versatility of the MITC approach and suggestions are made for further application areas. Furthermore, the new moist variant of the HS test can be considered a test case of intermediate

  8. The Role of Moist Processes in the Intrinsic Predictability of Indian Ocean Cyclones

    SciTech Connect (OSTI)

    Taraphdar, Sourav; Mukhopadhyay, P.; Leung, Lai-Yung R.; Zhang, Fuqing; Abhilash, S.; Goswami, B. N.

    2014-07-16

    The role of moist processes and the possibility of error cascade from cloud scale processes affecting the intrinsic predictable time scale of a high resolution convection permitting model within the environment of tropical cyclones (TCs) over the Indian region are investigated. Consistent with past studies of extra-tropical cyclones, it is demonstrated that moist processes play a major role in forecast error growth which may ultimately limit the intrinsic predictability of the TCs. Small errors in the initial conditions may grow rapidly and cascades from smaller scales to the larger scales through strong diabatic heating and nonlinearities associated with moist convection. Results from a suite of twin perturbation experiments for four tropical cyclones suggest that the error growth is significantly higher in cloud permitting simulation at 3.3 km resolutions compared to simulations at 3.3 km and 10 km resolution with parameterized convection. Convective parameterizations with prescribed convective time scales typically longer than the model time step allows the effects of microphysical tendencies to average out so convection responds to a smoother dynamical forcing. Without convective parameterizations, the finer-scale instabilities resolved at 3.3 km resolution and stronger vertical motion that results from the cloud microphysical parameterizations removing super-saturation at each model time step can ultimately feed the error growth in convection permitting simulations. This implies that careful considerations and/or improvements in cloud parameterizations are needed if numerical predictions are to be improved through increased model resolution. Rapid upscale error growth from convective scales may ultimately limit the intrinsic mesoscale predictability of the TCs, which further supports the needs for probabilistic forecasts of these events, even at the mesoscales.

  9. Drying rate and temperature profile for superheated steam vacuum drying and moist air drying of softwood lumber

    SciTech Connect (OSTI)

    Pang, S.; Dakin, M. [New Zealand Forest Research Inst., Ltd., Rotorua (New Zealand). Mfg. Technologies Portfolio

    1999-07-01

    Two charges of green radiata pine sapwood lumber were dried, ether using superheated steam under vacuum (90 C, 0.2 bar abs.) or conventionally using hot moist air (90/60 C). Due to low density of the drying medium under vacuum, the circulation velocity used was 10 m/s for superheated steam drying and 5.0 m/s for moist air drying, and in both cases, the flow was unidirectional. In drying, stack drying rate and wood temperatures were measured to examine the differences between the superheated steam drying and drying using hot moist air. The experimental results have shown that the stack edge board in superheated steam drying dried faster than in the hot moist air drying. Once again due to the low density of the steam under vacuum, a prolonged maximum temperature drop across load (TDAL) was observed in the superheated steam drying, however, the whole stack dried slower and the final moisture content distribution was more variable than for conventional hot moist air drying.

  10. Multi-temperature method for high-pressure sorption measurements on moist shales

    SciTech Connect (OSTI)

    Gasparik, Matus; Ghanizadeh, Amin; Gensterblum, Yves; Krooss, Bernhard M.

    2013-08-15

    A simple and effective experimental approach has been developed and tested to study the temperature dependence of high-pressure methane sorption in moist organic-rich shales. This method, denoted as multi-temperature (short multi-T) method, enables measuring multiple isotherms at varying temperatures in a single run. The measurement of individual sorption isotherms at different temperatures takes place in a closed system ensuring that the moisture content remains constant. The multi-T method was successfully tested for methane sorption on an organic-rich shale sample. Excess sorption isotherms for methane were measured at pressures of up to 25 MPa and at temperatures of 318.1 K, 338.1 K, and 348.1 K on dry and moisture-equilibrated samples. The measured isotherms were parameterized with a 3-parameter Langmuir-based excess sorption function, from which thermodynamic sorption parameters (enthalpy and entropy of adsorption) were obtained. Using these, we show that by taking explicitly into account water vapor as molecular species in the gas phase with temperature-dependent water vapor pressure during the experiment, more meaningful results are obtained with respect to thermodynamical considerations. The proposed method can be applied to any adsorbent system (coals, shales, industrial adsorbents) and any supercritical gas (e.g., CH{sub 4}, CO{sub 2}) and is particularly suitable for sorption measurements using the manometric (volumetric) method.

  11. Results of Scoping Studies for Determining Radiolytic Hydrogen Production from Moist CST and CST Slurries

    SciTech Connect (OSTI)

    Bibler, N.E.

    1998-12-11

    In support of the Salt Disposition team, scoping studies have been performed on the radiolysis of moist and aqueous slurries of Crystalline Silicotitanate(CST). If CST is used for removal of Cs-137 from SRS salt solutions, radiolysis of the water by Cs-137 on the CST will produce H2. Also it has been shown that the presence of a solid in the system can enhance the production of H2 by transferring absorbed energy from the solid to the water (1). As indicated in the test plan (2) for this scoping study, it is the intent of this study to determine if CST enhances the radiolytic production of H2 and to estimate the radiolytic hydrogen generation rate from an aqueous CST slurry in a column at the maximum expected Cs-137 loading on the CST.Initially several CST slurry systems were irradiated with Co-60 gamma rays and the radiolytic yield of H2 measured in terms of its G value (molecules of H2 produced per 100 eV of energy absorbed). Based on the results of these tests it was determined that CST did not enhance the radiolytic production of H2 by transferring energy to the water and causing it to decompose.Calculations were then performed to estimate the rate of H2 production from a process column 16ft. long by 5ft. in diameter containing CST that was fully loaded with Cs-137. The maximum rate of H2 production based on the G values measured in this study was one liter per minute at STP (0.04 cfm). This was for a 63 percent water/CST slurry with a G value of 0.2 molecules/100eV for H2 production and a loading of 1 gram of Cs-137 per 100 grams of resin. The present work also indicates that for a column containing salt solution and CST rather than water and CST, the rate would be 4X lower. This lower value is much more realistic.

  12. Cast Stone Oxidation Front Evaluation: Preliminary Results For Samples Exposed To Moist Air

    SciTech Connect (OSTI)

    Langton, C. A.; Almond, P. M.

    2013-11-26

    The rate of oxidation is important to the long-term performance of reducing salt waste forms because the solubility of some contaminants, e.g., technetium, is a function of oxidation state. TcO{sub 4}{sup −} in the salt solution is reduced to Tc(IV) and has been shown to react with ingredients in the waste form to precipitate low solubility sulfide and/or oxide phases. Upon exposure to oxygen, the compounds containing Tc(IV) oxidize to the pertechnetate ion, Tc(VII)O{sub 4}{sup −}, which is very soluble. Consequently the rate of technetium oxidation front advancement into a monolith and the technetium leaching profile as a function of depth from an exposed surface are important to waste form performance and ground water concentration predictions. An approach for measuring contaminant oxidation rate (effective contaminant specific oxidation rate) based on leaching of select contaminants of concern is described in this report. In addition, the relationship between reduction capacity and contaminant oxidation is addressed. Chromate (Cr(VI) was used as a non-radioactive surrogate for pertechnetate, Tc(VII), in Cast Stone samples prepared with 5 M Simulant. Cast Stone spiked with pertechnetate was also prepared and tested. Depth discrete subsamples spiked with Cr were cut from Cast Stone exposed to Savannah River Site (SRS) outdoor ambient temperature fluctuations and moist air. Depth discrete subsamples spiked with Tc-99 were cut from Cast Stone exposed to laboratory ambient temperature fluctuations and moist air. Similar conditions are expected to be encountered in the Cast Stone curing container. The leachability of Cr and Tc-99 and the reduction capacities, measured by the Angus-Glasser method, were determined for each subsample as a function of depth from the exposed surface. The results obtained to date were focused on continued method development and are preliminary and apply to the sample composition and curing / exposure conditions described in this report

  13. Improvement of Moist and Radiative Processes in Highly Parallel Atmospheric General Circulation Models: Validation and Development

    SciTech Connect (OSTI)

    Frank, William M.; Hack, James J.; Kiehl, Jeffrey T.

    1997-02-24

    Research on designing an integrated moist process parameterization package was carried. This work began with a study that coupled an ensemble of cloud models to a boundary layer model to examine the feasibility of such a methodology for linking boundary layer and cumulus parameterization schemes. The approach proved feasible, prompting research to design and evaluate a coupled parameterization package for GCMS. This research contributed to the development of an Integrated Cumulus Ensemble-Turbulence (ICET) parameterization package. This package incorporates a higher-order turbulence boundary layer that feeds information concerning updraft properties and the variances of temperature and water vapor to the cloud parameterizations. The cumulus ensemble model has been developed, and initial sensitivity tests have been performed in the single column model (SCM) version of CCM2. It is currently being coupled to a convective wake/gust front model. The major function of the convective wake/gust front model is to simulate the partitioning of the boundary layer into disturbed and undisturbed regions. A second function of this model is to predict the nonlinear enhancement of surface to air sensible heat and moisture fluxes that occur in convective regimes due to correlations between winds and anomalously cold, dry air from downdrafts in the gust front region. The third function of the convective wake/gust front model is to predict the amount of undisturbed boundary layer air lifted by the leading edge of the wake and the height to which this air is lifted. The development of the wake/gust front model has been completed, and it has done well in initial testing as a stand-alone component. The current task, to be completed by the end of the funding period, is to tie the wake model to a cumulus ensemble model and to install both components into the single column model version of CCM3 for evaluation. Another area of parametrization research has been focused on the representation

  14. Safety Basis Report

    SciTech Connect (OSTI)

    R.J. Garrett

    2002-01-14

    As part of the internal Integrated Safety Management Assessment verification process, it was determined that there was a lack of documentation that summarizes the safety basis of the current Yucca Mountain Project (YMP) site characterization activities. It was noted that a safety basis would make it possible to establish a technically justifiable graded approach to the implementation of the requirements identified in the Standards/Requirements Identification Document. The Standards/Requirements Identification Documents commit a facility to compliance with specific requirements and, together with the hazard baseline documentation, provide a technical basis for ensuring that the public and workers are protected. This Safety Basis Report has been developed to establish and document the safety basis of the current site characterization activities, establish and document the hazard baseline, and provide the technical basis for identifying structures, systems, and components (SSCs) that perform functions necessary to protect the public, the worker, and the environment from hazards unique to the YMP site characterization activities. This technical basis for identifying SSCs serves as a grading process for the implementation of programs such as Conduct of Operations (DOE Order 5480.19) and the Suspect/Counterfeit Items Program. In addition, this report provides a consolidated summary of the hazards analyses processes developed to support the design, construction, and operation of the YMP site characterization facilities and, therefore, provides a tool for evaluating the safety impacts of changes to the design and operation of the YMP site characterization activities.

  15. Technical Planning Basis

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-07-11

    The Guide assists DOE/NNSA field elements and operating contractors in identifying and analyzing hazards at facilities and sites to provide the technical planning basis for emergency management programs. Supersedes DOE G 151.1-1, Volume 2.

  16. Interaction of Plutonium with Diverse Materials in Moist Air and Nitrogen-Argon Atmospheres at Room Temperature

    SciTech Connect (OSTI)

    John M. Haschke; Raymond J. Martinez; Robert E. Pruner II; Barbara Martinez; Thomas H. Allen

    2001-04-01

    Chemical and radiolytic interactions of weapons-grade plutonium with metallic, inorganic, and hydrogenous materials in atmospheres containing moist air-argon mixtures have been characterized at room temperature from pressure-volume-temperature and mass spectrometric measurements of the gas phase. A reaction sequence controlled by kinetics and gas-phase composition is defined by correlating observed and known reaction rates. In all cases, O{sub 2} is eliminated first by the water-catalyzed Pu + O{sub 2} reaction and H{sub 2}O is then consumed by the Pu + H{sub 2}O reaction, producing a gas mixture of N{sub 2}, argon, and H{sub 2}. Hydrogen formed by the reaction of water and concurrent radiolysis of hydrogenous materials either reacts to form PuH{sub 2} or accumulates in the system. Accumulation of H{sub 2} is correlated with the presence of hydrogenous materials in liquid and volatile forms that are readily distributed over the plutonium surface. Areal rates of radiolytic H{sub 2} generation are determined and applied in showing that modest extents of H{sub 2} production are expected for hydrogenous solids if the contact area with plutonium is limited. The unpredictable nature of complex chemical systems is demonstrated by occurrence of the chloride-catalyzed Pu + H{sub 2}O reaction in some tests and hydride-catalyzed nitriding in another.

  17. Basis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    This has a number of advantages, such as reduced dataset requirements, ability to ... then solve for these coefficients using statistical correlations in the dataset. ...

  18. Basis

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that this equation is a difference- equation representation in the temporal domain of a first- order-in-time nonlinear partial differential equation. The co- efficient L k...

  19. CRAD, NNSA- Safety Basis (SB)

    Broader source: Energy.gov [DOE]

    CRAD for Safety Basis (SB). Criteria Review and Approach Documents (CRADs) that can be used to conduct a well-organized and thorough assessment of elements of safety and health programs.

  20. The Basis Code Development System

    Energy Science and Technology Software Center (OSTI)

    1994-03-15

    BASIS9.4 is a system for developing interactive computer programs in Fortran, with some support for C and C++ as well. Using BASIS9.4 you can create a program that has a sophisticated programming language as its user interface so that the user can set, calculate with, and plot, all the major variables in the program. The program author writes only the scientific part of the program; BASIS9.4 supplies an environment in which to exercise that scientificmore » programming which includes an interactive language, an interpreter, graphics, terminal logs, error recovery, macros, saving and retrieving variables, formatted I/O, and online documentation.« less

  1. Evaluation of moist processes during intense precipitation in km-scale NWP models using remote sensing and in-situ data: Impact of microphysics size distribution assumptions

    SciTech Connect (OSTI)

    VanWeverberg, K.; vanLipzig, N. P. M.; Delobbe, L.

    2011-02-01

    This study investigates the sensitivity of moist processes and surface precipitation during three extreme precipitation events over Belgium to the representation of rain, snow and hail size distributions in a bulk one-moment microphysics parameterisation scheme. Sensitivities included the use of empirically derived relations to calculate the slope parameter and diagnose the intercept parameter of the exponential snow and rain size distributions and sensitivities to the treatment of hail/graupel. A detailed evaluation of the experiments against various high temporal resolution and spatially distributed observational data was performed to understand how moist processes responded to the implemented size distribution modifications. Net vapor consumption by microphysical processes was found to be unaffected by snow or rain size distribution modifications, while it was reduced replacing formulations for hail by those typical for graupel, mainly due to intense sublimation of graupel. Cloud optical thickness was overestimated in all experiments and all cases, likely due to overestimated snow amounts. The overestimation slightly deteriorated by modifying the rain and snow size distributions due to increased snow depositional growth, while it was reduced by including graupel. The latter was mainly due to enhanced cloud water collection by graupel and reduced snow depositional growth. Radar reflectivity and cloud optical thickness could only be realistically represented by inclusion of graupel during a stratiform case, while hail was found indispensable to simulate the vertical reflectivity profile and the surface precipitation structure. Precipitation amount was not much altered by any of the modifications made and the general overestimation was only decreased slightly during a supercell convective case.

  2. Authorization basis requirements comparison report

    SciTech Connect (OSTI)

    Brantley, W.M.

    1997-08-18

    The TWRS Authorization Basis (AB) consists of a set of documents identified by TWRS management with the concurrence of DOE-RL. Upon implementation of the TWRS Basis for Interim Operation (BIO) and Technical Safety Requirements (TSRs), the AB list will be revised to include the BIO and TSRs. Some documents that currently form part of the AB will be removed from the list. This SD identifies each - requirement from those documents, and recommends a disposition for each to ensure that necessary requirements are retained when the AB is revised to incorporate the BIO and TSRs. This SD also identifies documents that will remain part of the AB after the BIO and TSRs are implemented. This document does not change the AB, but provides guidance for the preparation of change documentation.

  3. Hanford Generic Interim Safety Basis

    SciTech Connect (OSTI)

    Lavender, J.C.

    1994-09-09

    The purpose of this document is to identify WHC programs and requirements that are an integral part of the authorization basis for nuclear facilities that are generic to all WHC-managed facilities. The purpose of these programs is to implement the DOE Orders, as WHC becomes contractually obligated to implement them. The Hanford Generic ISB focuses on the institutional controls and safety requirements identified in DOE Order 5480.23, Nuclear Safety Analysis Reports.

  4. design basis threat | National Nuclear Security Administration

    National Nuclear Security Administration (NNSA)

    design basis threat Design Basis Threat NNSA has taken aggressive action to improve the security of its nuclear weapons material (often referred to as special nuclear material, or ...

  5. OSR encapsulation basis -- 100-KW

    SciTech Connect (OSTI)

    Meichle, R.H.

    1995-01-27

    The purpose of this report is to provide the basis for a change in the Operations Safety Requirement (OSR) encapsulated fuel storage requirements in the 105 KW fuel storage basin which will permit the handling and storing of encapsulated fuel in canisters which no longer have a water-free space in the top of the canister. The scope of this report is limited to providing the change from the perspective of the safety envelope (bases) of the Safety Analysis Report (SAR) and Operations Safety Requirements (OSR). It does not change the encapsulation process itself.

  6. Internal dosimetry technical basis manual

    SciTech Connect (OSTI)

    Not Available

    1990-12-20

    The internal dosimetry program at the Savannah River Site (SRS) consists of radiation protection programs and activities used to detect and evaluate intakes of radioactive material by radiation workers. Examples of such programs are: air monitoring; surface contamination monitoring; personal contamination surveys; radiobioassay; and dose assessment. The objectives of the internal dosimetry program are to demonstrate that the workplace is under control and that workers are not being exposed to radioactive material, and to detect and assess inadvertent intakes in the workplace. The Savannah River Site Internal Dosimetry Technical Basis Manual (TBM) is intended to provide a technical and philosophical discussion of the radiobioassay and dose assessment aspects of the internal dosimetry program. Detailed information on air, surface, and personal contamination surveillance programs is not given in this manual except for how these programs interface with routine and special bioassay programs.

  7. Beyond Design Basis Events | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Beyond Design Basis Events Beyond Design Basis Events Beyond Design Basis Events Following the March 2011 Fukushima Daiichi nuclear plant accident in Japan, DOE embarked upon several initiatives to investigate the safety posture of its nuclear facilities relative to beyond design basis events (BDBEs). These initiatives included issuing Safety Bulletin 2011-01, Events Beyond Design Safety Basis Analysis, and conducting two DOE nuclear safety workshops. DOE also issued two reports documenting the

  8. BASIS Set Exchange (BSE): Chemistry Basis Sets from the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL) Basis Set Library

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Feller, D; Schuchardt, Karen L.; Didier, Brett T.; Elsethagen, Todd; Sun, Lisong; Gurumoorthi, Vidhya; Chase, Jared; Li, Jun

    The Basis Set Exchange (BSE) provides a web-based user interface for downloading and uploading Gaussian-type (GTO) basis sets, including effective core potentials (ECPs), from the EMSL Basis Set Library. It provides an improved user interface and capabilities over its predecessor, the EMSL Basis Set Order Form, for exploring the contents of the EMSL Basis Set Library. The popular Basis Set Order Form and underlying Basis Set Library were originally developed by Dr. David Feller and have been available from the EMSL webpages since 1994. BSE not only allows downloading of the more than 200 Basis sets in various formats; it allows users to annotate existing sets and to upload new sets. (Specialized Interface)

  9. BASIS Set Exchange (BSE): Chemistry Basis Sets from the William R. Wiley Environmental Molecular Sciences Laboratory (EMSL) Basis Set Library

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Feller, D; Schuchardt, Karen L.; Didier, Brett T.; Elsethagen, Todd; Sun, Lisong; Gurumoorthi, Vidhya; Chase, Jared; Li, Jun

    The Basis Set Exchange (BSE) provides a web-based user interface for downloading and uploading Gaussian-type (GTO) basis sets, including effective core potentials (ECPs), from the EMSL Basis Set Library. It provides an improved user interface and capabilities over its predecessor, the EMSL Basis Set Order Form, for exploring the contents of the EMSL Basis Set Library. The popular Basis Set Order Form and underlying Basis Set Library were originally developed by Dr. David Feller and have been available from the EMSL webpages since 1994. BSE not only allows downloading of the more than 500 Basis sets in various formats; it allows users to annotate existing sets and to upload new sets. (Specialized Interface)

  10. Advanced Fuel Cycle Cost Basis

    SciTech Connect (OSTI)

    D. E. Shropshire; K. A. Williams; W. B. Boore; J. D. Smith; B. W. Dixon; M. Dunzik-Gougar; R. D. Adams; D. Gombert; E. Schneider

    2008-03-01

    This report, commissioned by the U.S. Department of Energy (DOE), provides a comprehensive set of cost data supporting a cost analysis for the relative economic comparison of options for use in the Advanced Fuel Cycle Initiative (AFCI) Program. The report describes the AFCI cost basis development process, reference information on AFCI cost modules, a procedure for estimating fuel cycle costs, economic evaluation guidelines, and a discussion on the integration of cost data into economic computer models. This report contains reference cost data for 25 cost modules—23 fuel cycle cost modules and 2 reactor modules. The cost modules were developed in the areas of natural uranium mining and milling, conversion, enrichment, depleted uranium disposition, fuel fabrication, interim spent fuel storage, reprocessing, waste conditioning, spent nuclear fuel (SNF) packaging, long-term monitored retrievable storage, near surface disposal of low-level waste (LLW), geologic repository and other disposal concepts, and transportation processes for nuclear fuel, LLW, SNF, transuranic, and high-level waste.

  11. Advanced Fuel Cycle Cost Basis

    SciTech Connect (OSTI)

    D. E. Shropshire; K. A. Williams; W. B. Boore; J. D. Smith; B. W. Dixon; M. Dunzik-Gougar; R. D. Adams; D. Gombert; E. Schneider

    2009-12-01

    This report, commissioned by the U.S. Department of Energy (DOE), provides a comprehensive set of cost data supporting a cost analysis for the relative economic comparison of options for use in the Advanced Fuel Cycle Initiative (AFCI) Program. The report describes the AFCI cost basis development process, reference information on AFCI cost modules, a procedure for estimating fuel cycle costs, economic evaluation guidelines, and a discussion on the integration of cost data into economic computer models. This report contains reference cost data for 25 cost modules—23 fuel cycle cost modules and 2 reactor modules. The cost modules were developed in the areas of natural uranium mining and milling, conversion, enrichment, depleted uranium disposition, fuel fabrication, interim spent fuel storage, reprocessing, waste conditioning, spent nuclear fuel (SNF) packaging, long-term monitored retrievable storage, near surface disposal of low-level waste (LLW), geologic repository and other disposal concepts, and transportation processes for nuclear fuel, LLW, SNF, transuranic, and high-level waste.

  12. Advanced Fuel Cycle Cost Basis

    SciTech Connect (OSTI)

    D. E. Shropshire; K. A. Williams; W. B. Boore; J. D. Smith; B. W. Dixon; M. Dunzik-Gougar; R. D. Adams; D. Gombert

    2007-04-01

    This report, commissioned by the U.S. Department of Energy (DOE), provides a comprehensive set of cost data supporting a cost analysis for the relative economic comparison of options for use in the Advanced Fuel Cycle Initiative (AFCI) Program. The report describes the AFCI cost basis development process, reference information on AFCI cost modules, a procedure for estimating fuel cycle costs, economic evaluation guidelines, and a discussion on the integration of cost data into economic computer models. This report contains reference cost data for 26 cost modules—24 fuel cycle cost modules and 2 reactor modules. The cost modules were developed in the areas of natural uranium mining and milling, conversion, enrichment, depleted uranium disposition, fuel fabrication, interim spent fuel storage, reprocessing, waste conditioning, spent nuclear fuel (SNF) packaging, long-term monitored retrievable storage, near surface disposal of low-level waste (LLW), geologic repository and other disposal concepts, and transportation processes for nuclear fuel, LLW, SNF, and high-level waste.

  13. Tank characterization technical sampling basis

    SciTech Connect (OSTI)

    Brown, T.M.

    1998-04-28

    Tank Characterization Technical Sampling Basis (this document) is the first step of an in place working process to plan characterization activities in an optimal manner. This document will be used to develop the revision of the Waste Information Requirements Document (WIRD) (Winkelman et al. 1997) and ultimately, to create sampling schedules. The revised WIRD will define all Characterization Project activities over the course of subsequent fiscal years 1999 through 2002. This document establishes priorities for sampling and characterization activities conducted under the Tank Waste Remediation System (TWRS) Tank Waste Characterization Project. The Tank Waste Characterization Project is designed to provide all TWRS programs with information describing the physical, chemical, and radiological properties of the contents of waste storage tanks at the Hanford Site. These tanks contain radioactive waste generated from the production of nuclear weapons materials at the Hanford Site. The waste composition varies from tank to tank because of the large number of chemical processes that were used when producing nuclear weapons materials over the years and because the wastes were mixed during efforts to better use tank storage space. The Tank Waste Characterization Project mission is to provide information and waste sample material necessary for TWRS to define and maintain safe interim storage and to process waste fractions into stable forms for ultimate disposal. This document integrates the information needed to address safety issues, regulatory requirements, and retrieval, treatment, and immobilization requirements. Characterization sampling to support tank farm operational needs is also discussed.

  14. Safety Basis Information System | Department of Energy

    Energy Savers [EERE]

    Click on the above link to log in to the Safety Basis web interface. "RESTRICTED; access ... Click on the above link to access the form to request access to the Safety Basis web ...

  15. Beyond Design Basis Event Pilot Evaluations

    Office of Energy Efficiency and Renewable Energy (EERE)

    This document provides Results and Recommendations for Improvements to Enhance Nuclear Safety at Department of Energy Nuclear Facilities based upon Beyond Design Basis Event Pilot Evaluations

  16. Structural basis for Tetrahymena telomerase processivity factor...

    Office of Scientific and Technical Information (OSTI)

    factor Teb1 binding to single-stranded telomeric-repeat DNA Citation Details In-Document Search Title: Structural basis for Tetrahymena telomerase processivity factor Teb1 ...

  17. Property:ExplorationBasis | Open Energy Information

    Open Energy Info (EERE)

    Text Description Exploration Basis Why was exploration work conducted in this area (e.g., USGS report of a geothermal resource, hot springs with geothemmetry indicating...

  18. Safety Basis Information System | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Request Click on the above link to access the form to request access to the Safety Basis web interface. If you need assistance logging in, please AU UserSupport. Contact Nimi Rao...

  19. Basis for UCNI | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    UCNI Basis for UCNI What documents contain the legal and policy foundations for the UCNI program? Section 148 of the Atomic Energy Act of 1954, as amended (42 U.S.C. 2011 et seq.), is the statutory basis for the UCNI program. 10 CFR Part 1017, Identification and Protection of Unclassified Controlled Nuclear Information specifies many detailed policies and requirements concerning the UCNI program. DOE O 471.1B, Identification and Protection of Unclassified Controlled Nuclear Information,

  20. Nanoplasmonics simulations at the basis set limit through completeness-optimized, local numerical basis sets

    SciTech Connect (OSTI)

    Rossi, Tuomas P. Sakko, Arto; Puska, Martti J.; Lehtola, Susi; Nieminen, Risto M.

    2015-03-07

    We present an approach for generating local numerical basis sets of improving accuracy for first-principles nanoplasmonics simulations within time-dependent density functional theory. The method is demonstrated for copper, silver, and gold nanoparticles that are of experimental interest but computationally demanding due to the semi-core d-electrons that affect their plasmonic response. The basis sets are constructed by augmenting numerical atomic orbital basis sets by truncated Gaussian-type orbitals generated by the completeness-optimization scheme, which is applied to the photoabsorption spectra of homoatomic metal atom dimers. We obtain basis sets of improving accuracy up to the complete basis set limit and demonstrate that the performance of the basis sets transfers to simulations of larger nanoparticles and nanoalloys as well as to calculations with various exchange-correlation functionals. This work promotes the use of the local basis set approach of controllable accuracy in first-principles nanoplasmonics simulations and beyond.

  1. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Basis for Activation of Cholera Toxin Structural Basis for Activation of Cholera Toxin Print Wednesday, 30 November 2005 00:00 Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit

  2. CRAD, Facility Safety- Nuclear Facility Safety Basis

    Office of Energy Efficiency and Renewable Energy (EERE)

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) that can be used for assessment of a contractor's Nuclear Facility Safety Basis.

  3. TWRS authorization basis configuration control summary

    SciTech Connect (OSTI)

    Mendoza, D.P.

    1997-12-26

    This document was developed to define the Authorization Basis management functional requirements for configuration control, to evaluate the management control systems currently in place, and identify any additional controls that may be required until the TWRS [Tank Waste Remediation System] Configuration Management system is fully in place.

  4. Review and Approval of Nuclear Facility Safety Basis and Safety Design Basis Documents

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2014-12-19

    This Standard describes a framework and the criteria to be used for approval of (1) safety basis documents, as required by 10 Code of Federal Regulation (C.F.R.) 830, Nuclear Safety Management, and (2) safety design basis documents, as required by Department of Energy (DOE) Standard (STD)-1189-2008, Integration of Safety into the Design Process.

  5. TECHNICAL BASIS DOCUMENT FOR NATURAL EVENT HAZARDS

    SciTech Connect (OSTI)

    KRIPPS, L.J.

    2006-07-31

    This technical basis document was developed to support the documented safety analysis (DSA) and describes the risk binning process and the technical basis for assigning risk bins for natural event hazard (NEH)-initiated accidents. The purpose of the risk binning process is to determine the need for safety-significant structures, systems, and components (SSC) and technical safety requirement (TSR)-level controls for a given representative accident or represented hazardous conditions based on an evaluation of the frequency and consequence. Note that the risk binning process is not applied to facility workers, because all facility worker hazardous conditions are considered for safety-significant SSCs and/or TSR-level controls.

  6. Radioactive Waste Management BasisApril 2006

    SciTech Connect (OSTI)

    Perkins, B K

    2011-08-31

    This Radioactive Waste Management Basis (RWMB) documents radioactive waste management practices adopted at Lawrence Livermore National Laboratory (LLNL) pursuant to Department of Energy (DOE) Order 435.1, Radioactive Waste Management. The purpose of this Radioactive Waste Management Basis is to describe the systematic approach for planning, executing, and evaluating the management of radioactive waste at LLNL. The implementation of this document will ensure that waste management activities at LLNL are conducted in compliance with the requirements of DOE Order 435.1, Radioactive Waste Management, and the Implementation Guide for DOE Manual 435.1-1, Radioactive Waste Management Manual. Technical justification is provided where methods for meeting the requirements of DOE Order 435.1 deviate from the DOE Manual 435.1-1 and Implementation Guide.

  7. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Basis for Activation of Cholera Toxin Print Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit (CTA1) triggers a series of events culminating in the massive efflux of

  8. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Basis for Activation of Cholera Toxin Print Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit (CTA1) triggers a series of events culminating in the massive efflux of

  9. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Basis for Activation of Cholera Toxin Print Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit (CTA1) triggers a series of events culminating in the massive efflux of

  10. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural Basis for Activation of Cholera Toxin Print Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit (CTA1) triggers a series of events culminating in the massive efflux of

  11. Review and Approval of Nuclear Facility Safety Basis and Safety Design Basis Documents

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SENSITIVE DOE-STD-1104-2014 December 2014 Superseding DOE-STD-1104-2009 DOE STANDARD REVIEW AND APPROVAL OF NUCLEAR FACILITY SAFETY BASIS AND SAFETY DESIGN BASIS DOCUMENTS U.S. Department of Energy AREA SAFT Washington, DC 20585 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. DOE-STD-1104-2014 i FOREWORD 1. This Standard describes a framework and the criteria to be used for approval of (1) safety basis documents, as required by 10 Code of Federal Regulation

  12. ORISE: The Medical Basis for Radiation-Accident Preparedness...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The Medical Basis for Radiation-Accident Preparedness: Medical Management Proceedings of the Fifth International REACTS Symposium on the Medical Basis for Radiation-Accident ...

  13. Nuclear Safety Basis Program Review Overview and Management Oversight...

    Office of Environmental Management (EM)

    Nuclear Safety Basis Program Review Overview and Management Oversight Standard Review Plan Nuclear Safety Basis Program Review Overview and Management Oversight Standard Review ...

  14. Structural Basis for the Interaction between Pyk2-FAT Domain...

    Office of Scientific and Technical Information (OSTI)

    Structural Basis for the Interaction between Pyk2-FAT Domain and Leupaxin LD Repeats Citation Details In-Document Search Title: Structural Basis for the Interaction between ...

  15. Heavy quarkonium in a holographic basis (Journal Article) | DOE...

    Office of Scientific and Technical Information (OSTI)

    Heavy quarkonium in a holographic basis Title: Heavy quarkonium in a holographic basis Authors: Li, Yang Search DOE PAGES for author "Li, Yang" Search DOE PAGES for ORCID ...

  16. A molecular basis for advanced materials in water treatment....

    Office of Scientific and Technical Information (OSTI)

    A molecular basis for advanced materials in water treatment. Citation Details In-Document Search Title: A molecular basis for advanced materials in water treatment. Authors: Rempe, ...

  17. Los Alamos National Laboratory fission basis (Conference) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Los Alamos National Laboratory fission basis Citation Details In-Document Search Title: Los Alamos National Laboratory fission basis You are accessing a document from the ...

  18. Technical Cost Modeling - Life Cycle Analysis Basis for Program...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Polymer ...

  19. Technical Cost Modeling - Life Cycle Analysis Basis for Program...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Life Cycle ...

  20. Technical Cost Modeling - Life Cycle Analysis Basis for Program...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    More Documents & Publications Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus ...

  1. Structural Basis of Prion Inhibition by Phenothiazine Compounds...

    Office of Scientific and Technical Information (OSTI)

    SciTech Connect Search Results Journal Article: Structural Basis of Prion Inhibition by Phenothiazine Compounds Citation Details In-Document Search Title: Structural Basis of Prion ...

  2. NDRPProtocolTechBasisCompiled020705.doc

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Basis Document for the Neutron Dose Reconstruction Project NEUTRON DOSE RECONSTRUCTION PROTOCOL Roger B. Falk, Joe M. Aldrich, Jerry Follmer, Nancy M. Daugherty, and Dr. Duane E. Hilmas Oak Ridge Institute of Science and Education and Dr. Phillip L. Chapman Department of Statistics, Colorado State University February 7, 2005 ORISE 05-0199 This document was produced under contract number DE-AC05-00OR22750 between the U.S. Department of Energy and Oak Ridge Associated Universities The authors of

  3. Technical Basis for PNNL Beryllium Inventory

    SciTech Connect (OSTI)

    Johnson, Michelle Lynn

    2014-07-09

    The Department of Energy (DOE) issued Title 10 of the Code of Federal Regulations Part 850, “Chronic Beryllium Disease Prevention Program” (the Beryllium Rule) in 1999 and required full compliance by no later than January 7, 2002. The Beryllium Rule requires the development of a baseline beryllium inventory of the locations of beryllium operations and other locations of potential beryllium contamination at DOE facilities. The baseline beryllium inventory is also required to identify workers exposed or potentially exposed to beryllium at those locations. Prior to DOE issuing 10 CFR 850, Pacific Northwest Nuclear Laboratory (PNNL) had documented the beryllium characterization and worker exposure potential for multiple facilities in compliance with DOE’s 1997 Notice 440.1, “Interim Chronic Beryllium Disease.” After DOE’s issuance of 10 CFR 850, PNNL developed an implementation plan to be compliant by 2002. In 2014, an internal self-assessment (ITS #E-00748) of PNNL’s Chronic Beryllium Disease Prevention Program (CBDPP) identified several deficiencies. One deficiency is that the technical basis for establishing the baseline beryllium inventory when the Beryllium Rule was implemented was either not documented or not retrievable. In addition, the beryllium inventory itself had not been adequately documented and maintained since PNNL established its own CBDPP, separate from Hanford Site’s program. This document reconstructs PNNL’s baseline beryllium inventory as it would have existed when it achieved compliance with the Beryllium Rule in 2001 and provides the technical basis for the baseline beryllium inventory.

  4. Review and Approval of Nuclear Facility Safety Basis and Safety Design Basis Documents

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SENSITIVE DOE-STD-1104-2009 May 2009 Superseding DOE-STD-1104-96 DOE STANDARD REVIEW AND APPROVAL OF NUCLEAR FACILITY SAFETY BASIS AND SAFETY DESIGN BASIS DOCUMENTS U.S. Department of Energy AREA SAFT Washington, DC 20585 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. DOE-STD-1104-2009 ii Available on the Department of Energy Technical Standards web page at http://www.hss.energy.gov/nuclearsafety/ns/techstds/ DOE-STD-1104-2009 iii CONTENTS FOREWORD

  5. Interim Basis for PCB Sampling and Analyses

    SciTech Connect (OSTI)

    BANNING, D.L.

    2001-03-20

    This document was developed as an interim basis for sampling and analysis of polychlorinated biphenyls (PCBs) and will be used until a formal data quality objective (DQO) document is prepared and approved. On August 31, 2000, the Framework Agreement for Management of Polychlorinated Biphenyls (PCBs) in Hanford Tank Waste was signed by the U.S. Department of Energy (DOE), the Environmental Protection Agency (EPA), and the Washington State Department of Ecology (Ecology) (Ecology et al. 2000). This agreement outlines the management of double shell tank (DST) waste as Toxic Substance Control Act (TSCA) PCB remediation waste based on a risk-based disposal approval option per Title 40 of the Code of Federal Regulations 761.61 (c). The agreement calls for ''Quantification of PCBs in DSTs, single shell tanks (SSTs), and incoming waste to ensure that the vitrification plant and other ancillary facilities PCB waste acceptance limits and the requirements of the anticipated risk-based disposal approval are met.'' Waste samples will be analyzed for PCBs to satisfy this requirement. This document describes the DQO process undertaken to assure appropriate data will be collected to support management of PCBs and is presented in a DQO format. The DQO process was implemented in accordance with the U.S. Environmental Protection Agency EPA QA/G4, Guidance for the Data Quality Objectives Process (EPA 1994) and the Data Quality Objectives for Sampling and Analyses, HNF-IP-0842, Rev. 1A, Vol. IV, Section 4.16 (Banning 1999).

  6. Interim Basis for PCB Sampling and Analyses

    SciTech Connect (OSTI)

    BANNING, D.L.

    2001-01-18

    This document was developed as an interim basis for sampling and analysis of polychlorinated biphenyls (PCBs) and will be used until a formal data quality objective (DQO) document is prepared and approved. On August 31, 2000, the Framework Agreement for Management of Polychlorinated Biphenyls (PCBs) in Hanford Tank Waste was signed by the US. Department of Energy (DOE), the Environmental Protection Agency (EPA), and the Washington State Department of Ecology (Ecology) (Ecology et al. 2000). This agreement outlines the management of double shell tank (DST) waste as Toxic Substance Control Act (TSCA) PCB remediation waste based on a risk-based disposal approval option per Title 40 of the Code of Federal Regulations 761.61 (c). The agreement calls for ''Quantification of PCBs in DSTs, single shell tanks (SSTs), and incoming waste to ensure that the vitrification plant and other ancillary facilities PCB waste acceptance limits and the requirements of the anticipated risk-based disposal approval are met.'' Waste samples will be analyzed for PCBs to satisfy this requirement. This document describes the DQO process undertaken to assure appropriate data will be collected to support management of PCBs and is presented in a DQO format. The DQO process was implemented in accordance with the U.S. Environmental Protection Agency EPA QAlG4, Guidance for the Data Quality Objectives Process (EPA 1994) and the Data Quality Objectives for Sampling and Analyses, HNF-IP-0842, Rev. 1 A, Vol. IV, Section 4.16 (Banning 1999).

  7. A radial basis function Galerkin method for inhomogeneous nonlocal diffusion

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lehoucq, Richard B.; Rowe, Stephen T.

    2016-02-01

    We introduce a discretization for a nonlocal diffusion problem using a localized basis of radial basis functions. The stiffness matrix entries are assembled by a special quadrature routine unique to the localized basis. Combining the quadrature method with the localized basis produces a well-conditioned, sparse, symmetric positive definite stiffness matrix. We demonstrate that both the continuum and discrete problems are well-posed and present numerical results for the convergence behavior of the radial basis function method. As a result, we explore approximating the solution to anisotropic differential equations by solving anisotropic nonlocal integral equations using the radial basis function method.

  8. PARFUME Theory and Model basis Report

    SciTech Connect (OSTI)

    Darrell L. Knudson; Gregory K Miller; G.K. Miller; D.A. Petti; J.T. Maki; D.L. Knudson

    2009-09-01

    The success of gas reactors depends upon the safety and quality of the coated particle fuel. The fuel performance modeling code PARFUME simulates the mechanical, thermal and physico-chemical behavior of fuel particles during irradiation. This report documents the theory and material properties behind vari¬ous capabilities of the code, which include: 1) various options for calculating CO production and fission product gas release, 2) an analytical solution for stresses in the coating layers that accounts for irradiation-induced creep and swelling of the pyrocarbon layers, 3) a thermal model that calculates a time-dependent temperature profile through a pebble bed sphere or a prismatic block core, as well as through the layers of each analyzed particle, 4) simulation of multi-dimensional particle behavior associated with cracking in the IPyC layer, partial debonding of the IPyC from the SiC, particle asphericity, and kernel migration (or amoeba effect), 5) two independent methods for determining particle failure probabilities, 6) a model for calculating release-to-birth (R/B) ratios of gaseous fission products that accounts for particle failures and uranium contamination in the fuel matrix, and 7) the evaluation of an accident condition, where a particle experiences a sudden change in temperature following a period of normal irradiation. The accident condi¬tion entails diffusion of fission products through the particle coating layers and through the fuel matrix to the coolant boundary. This document represents the initial version of the PARFUME Theory and Model Basis Report. More detailed descriptions will be provided in future revisions.

  9. Review and Approval of Nuclear Facility Safety Basis and Safety...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    104-2014, Review and Approval of Nuclear Facility Safety Basis and Safety Design Basis Documents by Website Administrator This Standard describes a framework and the criteria to be...

  10. CRAD, Integrated Safety Basis and Engineering Design Review ...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Integrated Safety Basis and Engineering Design Review - August 20, 2014 (EA CRAD 31-4, Rev. 0) CRAD, Integrated Safety Basis and Engineering Design Review - August 20, 2014 (EA...

  11. Office of Nuclear Safety Basis and Facility Design

    Broader source: Energy.gov [DOE]

    The Office of Nuclear Safety Basis & Facility Design establishes safety basis and facility design requirements and expectations related to analysis and design of nuclear facilities to ensure protection of workers and the public from the hazards associated with nuclear operations.

  12. Authorization basis status report (miscellaneous TWRS facilities, tanks and components)

    SciTech Connect (OSTI)

    Stickney, R.G.

    1998-04-29

    This report presents the results of a systematic evaluation conducted to identify miscellaneous TWRS facilities, tanks and components with potential needed authorization basis upgrades. It provides the Authorization Basis upgrade plan for those miscellaneous TWRS facilities, tanks and components identified.

  13. CRAD, Engineering Design and Safety Basis- December 22, 2009

    Broader source: Energy.gov [DOE]

    Engineering Design and Safety Basis Inspection Criteria, Inspection Activities, and Lines of Inquiry (HSS CRAD 64-19, Rev. 0)

  14. Nuclear Safety Basis Program Review Overview and Management Oversight

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Standard Review Plan | Department of Energy Safety Basis Program Review Overview and Management Oversight Standard Review Plan Nuclear Safety Basis Program Review Overview and Management Oversight Standard Review Plan This SRP, Nuclear Safety Basis Program Review, consists of five volumes. It provides information to help strengthen the technical rigor of line management oversight and federal monitoring of DOE nuclear facilities. It provides a primer on the safety basis development and

  15. Preprocessing Moist Lignocellulosic Biomass for Biorefinery Feedstocks

    SciTech Connect (OSTI)

    Neal Yancey; Christopher T. Wright; Craig Conner; J. Richard Hess

    2009-06-01

    Biomass preprocessing is one of the primary operations in the feedstock assembly system of a lignocellulosic biorefinery. Preprocessing is generally accomplished using industrial grinders to format biomass materials into a suitable biorefinery feedstock for conversion to ethanol and other bioproducts. Many factors affect machine efficiency and the physical characteristics of preprocessed biomass. For example, moisture content of the biomass as received from the point of production has a significant impact on overall system efficiency and can significantly affect the characteristics (particle size distribution, flowability, storability, etc.) of the size-reduced biomass. Many different grinder configurations are available on the market, each with advantages under specific conditions. Ultimately, the capacity and/or efficiency of the grinding process can be enhanced by selecting the grinder configuration that optimizes grinder performance based on moisture content and screen size. This paper discusses the relationships of biomass moisture with respect to preprocessing system performance and product physical characteristics and compares data obtained on corn stover, switchgrass, and wheat straw as model feedstocks during Vermeer HG 200 grinder testing. During the tests, grinder screen configuration and biomass moisture content were varied and tested to provide a better understanding of their relative impact on machine performance and the resulting feedstock physical characteristics and uniformity relative to each crop tested.

  16. Enterprise Assessments Review of the Delegation of Safety Basis Approval

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Authority for Hazard Category 1, 2, and 3 Nuclear Facilities - April 2016 | Department of Energy Review of the Delegation of Safety Basis Approval Authority for Hazard Category 1, 2, and 3 Nuclear Facilities - April 2016 Enterprise Assessments Review of the Delegation of Safety Basis Approval Authority for Hazard Category 1, 2, and 3 Nuclear Facilities - April 2016 April 2016 Enterprise Assessments Review of the Delegation of Safety Basis Approval Authority for Hazard Category 1, 2, and 3

  17. ORISE: The Medical Basis for Radiation-Accident Preparedness: Medical

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management (Published by REAC/TS) The Medical Basis for Radiation-Accident Preparedness: Medical Management Proceedings of the Fifth International REAC/TS Symposium on the Medical Basis for Radiation-Accident Preparedness and the Biodosimetry Workshop As part of its mission to provide continuing education for personnel responsible for treating radiation injuries, REAC/TS hosted the Fifth International REAC/TS Symposium on the Medical Basis for Radiation-Accident Preparedness symposium and

  18. Protocol for Enhanced Evaluations of Beyond Design Basis Events Supporting

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Implementation of Operating Experience Report 2013-01 | Department of Energy Protocol for Enhanced Evaluations of Beyond Design Basis Events Supporting Implementation of Operating Experience Report 2013-01 Protocol for Enhanced Evaluations of Beyond Design Basis Events Supporting Implementation of Operating Experience Report 2013-01 April, 2013 Protocol for Enhanced Evaluations of Beyond Design Basis Events Supporting Implementation of Operating Experience Report 2013-01 To support the

  19. Structural and Functional Basis for Inhibition of Erythrocyte...

    Office of Scientific and Technical Information (OSTI)

    Target Plasmodium falciparum EBA-175 Citation Details In-Document Search Title: Structural and Functional Basis for Inhibition of Erythrocyte Invasion by Antibodies that Target ...

  20. Protocol for Enhanced Evaluations of Beyond Design Basis Events...

    Energy Savers [EERE]

    Protocol for Enhanced Evaluations of Beyond Design Basis Events Supporting Implementation of Operating Experience Report 2013-01 Protocol for Enhanced Evaluations of Beyond Design ...

  1. Assessing Beyond Design Basis Seismic Events and Implications...

    Office of Environmental Management (EM)

    Defense Nuclear Facilities Safety Board Topics Covered: Department of Energy Approach to Natural Phenomena Hazards Analysis and Design (Seismic) Design Basis and Beyond Design...

  2. CRAD, Review of Safety Basis Development- January 31, 2013

    Broader source: Energy.gov [DOE]

    Review of Safety Basis Development for the Savannah River Site Salt Waste Processing Facility - Inspection Criteria, Approach, and Lines of Inquiry (HSS CRAD 45-57, Rev. 0)

  3. Technical Planning Basis - DOE Directives, Delegations, and Requiremen...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2, Technical Planning Basis by David Freshwater Functional areas: Defense Nuclear Facility Safety and Health Requirement, Safety and Security, The Guide assists DOENNSA field...

  4. Structural and Functional Basis for Broad-spectrum Neutralization...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structural and Functional Basis for Broad-spectrum Neutralization of Avian and Human ... globally that have little or no immunity, represents a grave threat to human health. ...

  5. Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus |

    Broader source: Energy.gov (indexed) [DOE]

    Department of Energy 1 DOE Hydrogen and Fuel Cells Program, and Vehicle Technologies Program Annual Merit Review and Peer Evaluation lm001_das_2011_o.pdf (305.88 KB) More Documents & Publications Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus Multi-Material Joining: Challenges and Opportunities

  6. Microsoft Word - Final_SRS_FTF_WD_Basis_March_2012

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    2-001 Revision 0 Basis for Section 3116 Determination for Closure of F-Tank Farm at the Savannah River Site March 2012 Basis for Section 3116 Determination DOE/SRS-WD-2012-001 for Closure of F-Tank Farm Revision 0 at the Savannah River Site March 2012 Page ii REVISION SUMMARY REV. # DESCRIPTION DATE OF ISSUE 0 Initial Issue March 2012 Basis for Section 3116 Determination DOE/SRS-WD-2012-001 for Closure of F-Tank Farm Revision 0 at the Savannah River Site March 2012 Page iii TABLE OF CONTENTS

  7. CRAD, Review of Safety Basis Development- October 11, 2012

    Broader source: Energy.gov [DOE]

    Review of Safety Basis Development for the Y-12 National Security Complex Uranium Processing Facility Inspection Criteria, Approach, and Lines of Inquiry (HSS CRAD 45-55, Rev. 0)

  8. SRS FTF Section 3116 Basis for Determination | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Basis for Section 3116 Determination for Closure of F-Tank Farm at the Savannah River Site. In accordance with NDAA Section 3116, certain waste from reprocessing of spent nuclear ...

  9. General Engineer/Physical Scientist (Safety Basis Engineer/Scientist)

    Broader source: Energy.gov [DOE]

    A successful candidate in this position will serve as an authority in the safety basis functional area. The incumbent is responsible for managing, coordinating, and authorizing work in the context...

  10. Structural basis for ubiquitin-mediated antiviral signal activation...

    Office of Scientific and Technical Information (OSTI)

    Title: Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I Authors: Peisley, Alys ; Wu, Bin ; Xu, Hui ; Chen, Zhijian J. ; Hur , Sun 1 ; HHMI) 2 ; ...

  11. Basis for Section 3116 Determination for Salt Waste Disposal...

    Office of Environmental Management (EM)

    WD-2005-001 January 2006 Basis for Section 3116 Determination for Salt Waste Disposal at ......... 28 4.0 THE WASTE DOES NOT REQUIRE PERMANENT ISOLATION IN A ...

  12. Structural basis for the antibody neutralization of Herpes simplex...

    Office of Scientific and Technical Information (OSTI)

    of Herpes simplex virus Citation Details In-Document Search Title: Structural basis for the antibody neutralization of Herpes simplex virus The gD-E317-Fab complex ...

  13. Advanced Test Reactor Design Basis Reconstitution Project Issue Resolution Process

    SciTech Connect (OSTI)

    Steven D. Winter; Gregg L. Sharp; William E. Kohn; Richard T. McCracken

    2007-05-01

    The Advanced Test Reactor (ATR) Design Basis Reconstitution Program (DBRP) is a structured assessment and reconstitution of the design basis for the ATR. The DBRP is designed to establish and document the ties between the Document Safety Analysis (DSA), design basis, and actual system configurations. Where the DBRP assessment team cannot establish a link between these three major elements, a gap is identified. Resolutions to identified gaps represent configuration management and design basis recovery actions. The proposed paper discusses the process being applied to define, evaluate, report, and address gaps that are identified through the ATR DBRP. Design basis verification may be performed or required for a nuclear facility safety basis on various levels. The process is applicable to large-scale design basis reconstitution efforts, such as the ATR DBRP, or may be scaled for application on smaller projects. The concepts are applicable to long-term maintenance of a nuclear facility safety basis and recovery of degraded safety basis components. The ATR DBRP assessment team has observed numerous examples where a clear and accurate link between the DSA, design basis, and actual system configuration was not immediately identifiable in supporting documentation. As a result, a systematic approach to effectively document, prioritize, and evaluate each observation is required. The DBRP issue resolution process provides direction for consistent identification, documentation, categorization, and evaluation, and where applicable, entry into the determination process for a potential inadequacy in the safety analysis (PISA). The issue resolution process is a key element for execution of the DBRP. Application of the process facilitates collection, assessment, and reporting of issues identified by the DBRP team. Application of the process results in an organized database of safety basis gaps and prioritized corrective action planning and resolution. The DBRP team follows the ATR

  14. Technical Basis Document for PFP Area Monitoring Dosimetry Program

    SciTech Connect (OSTI)

    COOPER, J.R.

    2000-04-17

    This document describes the phantom dosimetry used for the PFP Area Monitoring program and establishes the basis for the Plutonium Finishing Plant's (PFP) area monitoring dosimetry program in accordance with the following requirements: Title 10, Code of Federal Regulations (CFR), part 835, ''Occupational Radiation Protection'' Part 835.403; Hanford Site Radiological Control Manual (HSRCM-1), Part 514; HNF-PRO-382, Area Dosimetry Program; and PNL-MA-842, Hanford External Dosimetry Technical Basis Manual.

  15. Development of design basis capacity for SNF project systems

    SciTech Connect (OSTI)

    Pajunen, A.L.

    1996-02-27

    An estimate of the design capacity for Spent Nuclear Fuel Project systems producing Multi-Canister Overpacks is developed based on completing fuel processing in a two year period. The design basis capacity for systems relates the desired annual processing rate to potential operating inefficiencies which may be actually experienced to project a design capacity for systems. The basis for estimating operating efficiency factors is described. Estimates of the design basis capacity were limited to systems actually producing the Multi-Canister Overpack. These systems include Fuel Retrieval, K Basin SNF Vacuum Drying, Canister Storage Building support for Staging and Storage, and Hot Vacuum conditioning. The capacity of other systems are assumed to be derived from these system capacities such that systems producing a Multi-Canister Overpack are not constrained.

  16. Solar Power Tower Design Basis Document, Revision 0

    SciTech Connect (OSTI)

    ZAVOICO,ALEXIS B.

    2001-07-01

    This report contains the design basis for a generic molten-salt solar power tower. A solar power tower uses a field of tracking mirrors (heliostats) that redirect sunlight on to a centrally located receiver mounted on top a tower, which absorbs the concentrated sunlight. Molten nitrate salt, pumped from a tank at ground level, absorbs the sunlight, heating it up to 565 C. The heated salt flows back to ground level into another tank where it is stored, then pumped through a steam generator to produce steam and make electricity. This report establishes a set of criteria upon which the next generation of solar power towers will be designed. The report contains detailed criteria for each of the major systems: Collector System, Receiver System, Thermal Storage System, Steam Generator System, Master Control System, and Electric Heat Tracing System. The Electric Power Generation System and Balance of Plant discussions are limited to interface requirements. This design basis builds on the extensive experience gained from the Solar Two project and includes potential design innovations that will improve reliability and lower technical risk. This design basis document is a living document and contains several areas that require trade-studies and design analysis to fully complete the design basis. Project- and site-specific conditions and requirements will also resolve open To Be Determined issues.

  17. CRAD, Safety Basis- Idaho MF-628 Drum Treatment Facility

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for a May 2007 readiness assessment of the Safety Basis at the Advanced Mixed Waste Treatment Project.

  18. Canister Storage Building (CSB) Design Basis Accident Analysis Documentation

    SciTech Connect (OSTI)

    CROWE, R.D.

    1999-09-09

    This document provides the detailed accident analysis to support ''HNF-3553, Spent Nuclear Fuel Project Final Safety, Analysis Report, Annex A,'' ''Canister Storage Building Final Safety Analysis Report.'' All assumptions, parameters, and models used to provide the analysis of the design basis accidents are documented to support the conclusions in the Canister Storage Building Final Safety Analysis Report.

  19. Cold Vacuum Drying (CVD) Facility Design Basis Accident Analysis Documentation

    SciTech Connect (OSTI)

    PIEPHO, M.G.

    1999-10-20

    This document provides the detailed accident analysis to support HNF-3553, Annex B, Spent Nuclear Fuel Project Final Safety Analysis Report, ''Cold Vacuum Drying Facility Final Safety Analysis Report (FSAR).'' All assumptions, parameters and models used to provide the analysis of the design basis accidents are documented to support the conclusions in the FSAR.

  20. Canister Storage Building (CSB) Design Basis Accident Analysis Documentation

    SciTech Connect (OSTI)

    CROWE, R.D.; PIEPHO, M.G.

    2000-03-23

    This document provided the detailed accident analysis to support HNF-3553, Spent Nuclear Fuel Project Final Safety Analysis Report, Annex A, ''Canister Storage Building Final Safety Analysis Report''. All assumptions, parameters, and models used to provide the analysis of the design basis accidents are documented to support the conclusions in the Canister Storage Building Final Safety Analysis Report.

  1. Canister storage building design basis accident analysis documentation

    SciTech Connect (OSTI)

    KOPELIC, S.D.

    1999-02-25

    This document provides the detailed accident analysis to support HNF-3553, Spent Nuclear Fuel Project Final Safety Analysis Report, Annex A, ''Canister Storage Building Final Safety Analysis Report.'' All assumptions, parameters, and models used to provide the analysis of the design basis accidents are documented to support the conclusions in the Canister Storage Building Final Safety Analysis Report.

  2. CRAD, Safety Basis- Idaho Accelerated Retrieval Project Phase II

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for a February 2006 Commencement of Operations assessment of the Safety Basis at the Idaho Accelerated Retrieval Project Phase II.

  3. Design-Load Basis for LANL Structures, Systems, and Components

    SciTech Connect (OSTI)

    I. Cuesta

    2004-09-01

    This document supports the recommendations in the Los Alamos National Laboratory (LANL) Engineering Standard Manual (ESM), Chapter 5--Structural providing the basis for the loads, analysis procedures, and codes to be used in the ESM. It also provides the justification for eliminating the loads to be considered in design, and evidence that the design basis loads are appropriate and consistent with the graded approach required by the Department of Energy (DOE) Code of Federal Regulation Nuclear Safety Management, 10, Part 830. This document focuses on (1) the primary and secondary natural phenomena hazards listed in DOE-G-420.1-2, Appendix C, (2) additional loads not related to natural phenomena hazards, and (3) the design loads on structures during construction.

  4. Industrial ecology: A basis for sustainable relations and cooperation

    SciTech Connect (OSTI)

    Blades, K.

    1996-07-19

    The Commission for Environmental Cooperation (CEC) seeks to address, in a cooperative manner, the environmental issues affecting the North American region and understand the linkages between environment and economy. Broadly, the goal of the CEC can be thought of as an attempt to achieve a sustainable economy concomitantly with continued economic, cultural, and technological evolution. The emerging field of industrial ecology provides a useful means for balancing the environmental and economical objectives of NAFTA. As NAFTA stimulates economic cooperation and growth, we must collectively develop mechanisms that enhance the environmental quality of the region. LLNL`s effort in industrial ecology provides the scientific basis and innovative use of technology to reconcile environmental and economic concerns. Nevertheless, these are not issues which can be resolved by a single institution. Efficient use of the linkages established by NAFTA is necessary to nurture our regional partnership which forms the basis for a sustainable environment, economy and relationship.

  5. Basis for NGNP Reactor Design Down-Selection

    SciTech Connect (OSTI)

    L.E. Demick

    2011-11-01

    The purpose of this paper is to identify the extent of technology development, design and licensing maturity anticipated to be required to credibly identify differences that could make a technical choice practical between the prismatic and pebble bed reactor designs. This paper does not address a business decision based on the economics, business model and resulting business case since these will vary based on the reactor application. The selection of the type of reactor, the module ratings, the number of modules, the configuration of the balance of plant and other design selections will be made on the basis of optimizing the Business Case for the application. These are not decisions that can be made on a generic basis.

  6. Resilient Control Systems Practical Metrics Basis for Defining Mission Impact

    SciTech Connect (OSTI)

    Craig G. Rieger

    2014-08-01

    "Resilience” describes how systems operate at an acceptable level of normalcy despite disturbances or threats. In this paper we first consider the cognitive, cyber-physical interdependencies inherent in critical infrastructure systems and how resilience differs from reliability to mitigate these risks. Terminology and metrics basis are provided to integrate the cognitive, cyber-physical aspects that should be considered when defining solutions for resilience. A practical approach is taken to roll this metrics basis up to system integrity and business case metrics that establish “proper operation” and “impact.” A notional chemical processing plant is the use case for demonstrating how the system integrity metrics can be applied to establish performance, and

  7. Design Basis Threat | National Nuclear Security Administration | (NNSA)

    National Nuclear Security Administration (NNSA)

    Design Basis Threat NNSA has taken aggressive action to improve the security of its nuclear weapons material (often referred to as special nuclear material, or SNM) and nuclear weapons in its custody. NNSA has taken aggressive action to improve the security of its nuclear weapons material (often referred to as special nuclear material, or SNM) and nuclear weapons in its custody. NNSA has taken aggressive action to improve the security of its nuclear weapons material (often referred to as special

  8. Online Monitoring Technical Basis and Analysis Framework for Large Power

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Transformers; Interim Report for FY 2012 | Department of Energy for Large Power Transformers; Interim Report for FY 2012 Online Monitoring Technical Basis and Analysis Framework for Large Power Transformers; Interim Report for FY 2012 The Light Water Reactor Sustainability Program is a research, development, and deployment program sponsored by the U.S. Department of Energy Office of Nuclear Energy. The program is operated in collaboration with the Electric Power Research Institute's (EPRI's)

  9. Modeling the Molecular Basis of Parkinson's Disease | Argonne Leadership

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Computing Facility Alpha-synuclein pentamer constructed with 4ns molecular dynamics (MD) conformers after equilibration on the membrane with MD. Alpha-synuclein pentamer constructed with 4ns molecular dynamics (MD) conformers after equilibration on the membrane with MD. Modeling the Molecular Basis of Parkinson's Disease PI Name: Igor Tsigelny PI Email: itsigeln@ucsd.edu Institution: University of California-San Diego/SDSC Allocation Program: INCITE Allocation Hours at ALCF: 1.2 Million

  10. Interim Safety Basis for Fuel Supply Shutdown Facility

    SciTech Connect (OSTI)

    BENECKE, M.W.

    2000-09-07

    This ISB, in conjunction with the IOSR, provides the required basis for interim operation or restrictions on interim operations and administrative controls for the facility until a SAR is prepared in accordance with the new requirements or the facility is shut down. It is concluded that the risks associated with tha current and anticipated mode of the facility, uranium disposition, clean up, and transition activities required for permanent closure, are within risk guidelines.

  11. Basis functions for electronic structure calculations on spheres

    SciTech Connect (OSTI)

    Gill, Peter M. W. Loos, Pierre-François Agboola, Davids

    2014-12-28

    We introduce a new basis function (the spherical Gaussian) for electronic structure calculations on spheres of any dimension D. We find general expressions for the one- and two-electron integrals and propose an efficient computational algorithm incorporating the Cauchy-Schwarz bound. Using numerical calculations for the D = 2 case, we show that spherical Gaussians are more efficient than spherical harmonics when the electrons are strongly localized.

  12. Auxiliary basis expansions for large-scale electronic structure calculations

    SciTech Connect (OSTI)

    Jung, Yousung; Sodt, Alexander; Gill, Peter W.M.; Head-Gordon, Martin

    2005-04-04

    One way to reduce the computational cost of electronic structure calculations is to employ auxiliary basis expansions to approximate 4 center integrals in terms of 2 and 3-center integrals, usually using the variationally optimum Coulomb metric to determine the expansion coefficients. However the long-range decay behavior of the auxiliary basis expansion coefficients has not been characterized. We find that this decay can be surprisingly slow. Numerical experiments on linear alkanes and a toy model both show that the decay can be as slow as 1/r in the distance between the auxiliary function and the fitted charge distribution. The Coulomb metric fitting equations also involve divergent matrix elements for extended systems treated with periodic boundary conditions. An attenuated Coulomb metric that is short-range can eliminate these oddities without substantially degrading calculated relative energies. The sparsity of the fit coefficients is assessed on simple hydrocarbon molecules, and shows quite early onset of linear growth in the number of significant coefficients with system size using the attenuated Coulomb metric. This means it is possible to design linear scaling auxiliary basis methods without additional approximations to treat large systems.

  13. 5th International REAC/TS Symposium: The Medical Basis for Radiation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ...TS Symposium: The Medical Basis for Radiation Accident Preparedness Skip site ...TS Symposium: The Medical Basis for Radiation Accident Preparedness Sept. 27-29, 2011 ...

  14. CRAD, Safety Basis Upgrade Review (DOE-STD-3009-2014) - May 15...

    Office of Environmental Management (EM)

    1) provides objectives, criteria, and approaches for establishing and maintaining the safety basis at nuclear facilities. CRAD, Safety Basis Upgrade Review (DOE-STD-3009-2014)...

  15. AUDIT REPORT Follow-up on Nuclear Safety: Safety Basis and Quality...

    Broader source: Energy.gov (indexed) [DOE]

    Nuclear Safety: Safety Basis and Quality Assurance at the Los Alamos National Laboratory ... INFORMATION: Audit Report: "Follow-up on Nuclear Safety: Safety Basis and Quality ...

  16. RELEASE OF DRIED RADIOACTIVE WASTE MATERIALS TECHNICAL BASIS DOCUMENT

    SciTech Connect (OSTI)

    KOZLOWSKI, S.D.

    2007-05-30

    This technical basis document was developed to support RPP-23429, Preliminary Documented Safety Analysis for the Demonstration Bulk Vitrification System (PDSA) and RPP-23479, Preliminary Documented Safety Analysis for the Contact-Handled Transuranic Mixed (CH-TRUM) Waste Facility. The main document describes the risk binning process and the technical basis for assigning risk bins to the representative accidents involving the release of dried radioactive waste materials from the Demonstration Bulk Vitrification System (DBVS) and to the associated represented hazardous conditions. Appendices D through F provide the technical basis for assigning risk bins to the representative dried waste release accident and associated represented hazardous conditions for the Contact-Handled Transuranic Mixed (CH-TRUM) Waste Packaging Unit (WPU). The risk binning process uses an evaluation of the frequency and consequence of a given representative accident or represented hazardous condition to determine the need for safety structures, systems, and components (SSC) and technical safety requirement (TSR)-level controls. A representative accident or a represented hazardous condition is assigned to a risk bin based on the potential radiological and toxicological consequences to the public and the collocated worker. Note that the risk binning process is not applied to facility workers because credible hazardous conditions with the potential for significant facility worker consequences are considered for safety-significant SSCs and/or TSR-level controls regardless of their estimated frequency. The controls for protection of the facility workers are described in RPP-23429 and RPP-23479. Determination of the need for safety-class SSCs was performed in accordance with DOE-STD-3009-94, Preparation Guide for US. Department of Energy Nonreactor Nuclear Facility Documented Safety Analyses, as described below.

  17. Guidance For Preparatioon of Basis For Interim Operation (BIO) Documents

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3011-2002 December 2002 Superceding DOE-STD-3011-94 November 1994 DOE STANDARD GUIDANCE FOR PREPARATION OF BASIS FOR INTERIM OPERATION (BIO) DOCUMENTS U.S. Department of Energy AREA SAFT Washington, D.C. 20585 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. NOT MEASUREMENT SENSITIVE DOE-STD-3011-2002 ii This document has been reproduced directly from the best available copy. Available to DOE and DOE contractors from ES&H Technical Information Services, U.S.

  18. Preparation of Safety Basis Documents for Transuranic (TRU) Waste Facilities

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    5506-2007 April 2007 DOE STANDARD Preparation of Safety Basis Documents for Transuranic (TRU) Waste Facilities U.S. Department of Energy Washington, D.C. 20585 AREA-SAFT DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. DOE-STD-5506-2007 ii Available on the Department of Energy Technical Standards Program Web Site at Http://tis.eh.doe.gov/techstds/ DOE-STD-5506-2007 iii Foreword This Standard provides analytical assumptions and methods, as well as hazard controls

  19. The Bender-Dunne basis operators as Hilbert space operators

    SciTech Connect (OSTI)

    Bunao, Joseph; Galapon, Eric A. E-mail: eric.galapon@upd.edu.ph

    2014-02-15

    The Bender-Dunne basis operators, T{sub ?m,n}=2{sup ?n}?{sub k=0}{sup n}(n/k )q{sup k}p{sup ?m}q{sup n?k} where q and p are the position and momentum operators, respectively, are formal integral operators in position representation in the entire real line R for positive integers n and m. We show, by explicit construction of a dense domain, that the operators T{sub ?m,n}'s are densely defined operators in the Hilbert space L{sup 2}(R)

  20. Hanford External Dosimetry Technical Basis Manual PNL-MA-842

    SciTech Connect (OSTI)

    Rathbone, Bruce A.

    2005-02-25

    The Hanford External Dosimetry Technical Basis Manual PNL-MA-842 documents the design and implementation of the external dosimetry system used at Hanford. The manual describes the dosimeter design, processing protocols, dose calculation methodology, radiation fields encountered, dosimeter response characteristics, limitations of dosimeter design under field conditions, and makes recommendations for effective use of the dosimeters in the field. The manual describes the technical basis for the dosimetry system in a manner intended to help ensure defensibility of the dose of record at Hanford and to demonstrate compliance with 10 CFR 835, DOELAP, DOE-RL, ORP, PNSO, and Hanford contractor requirements. The dosimetry system is operated by PNNL’s Hanford External Dosimetry Program which provides dosimetry services to all Hanford contractors. The primary users of this manual are DOE and DOE contractors at Hanford using the dosimetry services of PNNL. Development and maintenance of this manual is funded directly by DOE and DOE contractors. Its contents have been reviewed and approved by DOE and DOE contractors at Hanford through the Hanford Personnel Dosimetry Advisory Committee which is chartered and chaired by DOE-RL and serves as means of coordinating dosimetry practices across contractors at Hanford. This manual was established in 1996. Since inception, it has been revised many times and maintained by PNNL as a controlled document with controlled distribution. Rev. 0 marks the first revision to be released through PNNL’s Electronic Records & Information Capture Architecture (ERICA) database.

  1. Cold Vacuum Drying facility design basis accident analysis documentation

    SciTech Connect (OSTI)

    CROWE, R.D.

    2000-08-08

    This document provides the detailed accident analysis to support HNF-3553, Annex B, Spent Nuclear Fuel Project Final Safety Analysis Report (FSAR), ''Cold Vacuum Drying Facility Final Safety Analysis Report.'' All assumptions, parameters, and models used to provide the analysis of the design basis accidents are documented to support the conclusions in the FSAR. The calculations in this document address the design basis accidents (DBAs) selected for analysis in HNF-3553, ''Spent Nuclear Fuel Project Final Safety Analysis Report'', Annex B, ''Cold Vacuum Drying Facility Final Safety Analysis Report.'' The objective is to determine the quantity of radioactive particulate available for release at any point during processing at the Cold Vacuum Drying Facility (CVDF) and to use that quantity to determine the amount of radioactive material released during the DBAs. The radioactive material released is used to determine dose consequences to receptors at four locations, and the dose consequences are compared with the appropriate evaluation guidelines and release limits to ascertain the need for preventive and mitigative controls.

  2. Electronic structure basis for the extraordinary magnetoresistance in WTe2

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pletikosić, I.; Ali, Mazhar N.; Fedorov, A. V.; Cava, R. J.; Valla, T.

    2014-11-19

    The electronic structure basis of the extremely large magnetoresistance in layered non-magnetic tungsten ditelluride has been investigated by angle-resolved photoelectron spectroscopy. Hole and electron pockets of approximately the same size were found at the Fermi level, suggesting that carrier compensation should be considered the primary source of the effect. The material exhibits a highly anisotropic, quasi one-dimensional Fermi surface from which the pronounced anisotropy of the magnetoresistance follows. As a result, a change in the Fermi surface with temperature was found and a high-density-of-states band that may take over conduction at higher temperatures and cause the observed turn-on behavior ofmore » the magnetoresistance in WTe₂ was identified.« less

  3. Draft Geologic Disposal Requirements Basis for STAD Specification

    SciTech Connect (OSTI)

    Ilgen, Anastasia G.; Bryan, Charles R.; Hardin, Ernest

    2015-03-25

    This document provides the basis for requirements in the current version of Performance Specification for Standardized Transportation, Aging, and Disposal Canister Systems, (FCRD-NFST-2014-0000579) that are driven by storage and geologic disposal considerations. Performance requirements for the Standardized Transportation, Aging, and Disposal (STAD) canister are given in Section 3.1 of that report. Here, the requirements are reviewed and the rationale for each provided. Note that, while FCRD-NFST-2014-0000579 provides performance specifications for other components of the STAD storage system (e.g. storage overpack, transfer and transportation casks, and others), these have no impact on the canister performance during disposal, and are not discussed here.

  4. Electronic structure basis for the titanic magnetoresistance in WTe?

    SciTech Connect (OSTI)

    Pletikosic, I.; Ali, Mazhar N.; Fedorov, A. V.; Cava, R. J.; Valla, T.

    2014-11-19

    The electronic structure basis of the extremely large magnetoresistance in layered non-magnetic tungsten ditelluride has been investigated by angle-resolved photoelectron spectroscopy. Hole and electron pockets of approximately the same size were found at the Fermi level, suggesting that carrier compensation should be considered the primary source of the effect. The material exhibits a highly anisotropic, quasi one-dimensional Fermi surface from which the pronounced anisotropy of the magnetoresistance follows. A change in the Fermi surface with temperature was found and a high-density-of-states band that may take over conduction at higher temperatures and cause the observed turn-on behavior of the magnetoresistance in WTe? was identified.

  5. Electronic structure basis for the titanic magnetoresistance in WTe?

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pletikosic, I.; Ali, Mazhar N.; Fedorov, A. V.; Cava, R. J.; Valla, T.

    2014-11-19

    The electronic structure basis of the extremely large magnetoresistance in layered non-magnetic tungsten ditelluride has been investigated by angle-resolved photoelectron spectroscopy. Hole and electron pockets of approximately the same size were found at the Fermi level, suggesting that carrier compensation should be considered the primary source of the effect. The material exhibits a highly anisotropic, quasi one-dimensional Fermi surface from which the pronounced anisotropy of the magnetoresistance follows. A change in the Fermi surface with temperature was found and a high-density-of-states band that may take over conduction at higher temperatures and cause the observed turn-on behavior of the magnetoresistance inmoreWTe? was identified.less

  6. Hanford External Dosimetry Technical Basis Manual PNL-MA-842

    SciTech Connect (OSTI)

    Rathbone, Bruce A.

    2009-08-28

    The Hanford External Dosimetry Technical Basis Manual PNL-MA-842 documents the design and implementation of the external dosimetry system used at Hanford. The manual describes the dosimeter design, processing protocols, dose calculation methodology, radiation fields encountered, dosimeter response characteristics, limitations of dosimeter design under field conditions, and makes recommendations for effective use of the dosimeters in the field. The manual describes the technical basis for the dosimetry system in a manner intended to help ensure defensibility of the dose of record at Hanford and to demonstrate compliance with 10 CFR 835, DOELAP, DOE-RL, ORP, PNSO, and Hanford contractor requirements. The dosimetry system is operated by PNNL’s Hanford External Dosimetry Program (HEDP) which provides dosimetry services to all Hanford contractors. The primary users of this manual are DOE and DOE contractors at Hanford using the dosimetry services of PNNL. Development and maintenance of this manual is funded directly by DOE and DOE contractors. Its contents have been reviewed and approved by DOE and DOE contractors at Hanford through the Hanford Personnel Dosimetry Advisory Committee (HPDAC) which is chartered and chaired by DOE-RL and serves as means of coordinating dosimetry practices across contractors at Hanford. This manual was established in 1996. Since inception, it has been revised many times and maintained by PNNL as a controlled document with controlled distribution. The first revision to be released through PNNL’s Electronic Records & Information Capture Architecture (ERICA) database was designated Revision 0. Revision numbers that are whole numbers reflect major revisions typically involving changes to all chapters in the document. Revision numbers that include a decimal fraction reflect minor revisions, usually restricted to selected chapters or selected pages in the document.

  7. Hanford External Dosimetry Technical Basis Manual PNL-MA-842

    SciTech Connect (OSTI)

    Rathbone, Bruce A.

    2011-04-04

    The Hanford External Dosimetry Technical Basis Manual PNL-MA-842 documents the design and implementation of the external dosimetry system used at the U.S. Department of Energy (DOE) Hanford site. The manual describes the dosimeter design, processing protocols, dose calculation methodology, radiation fields encountered, dosimeter response characteristics, limitations of dosimeter design under field conditions, and makes recommendations for effective use of the dosimeters in the field. The manual describes the technical basis for the dosimetry system in a manner intended to help ensure defensibility of the dose of record at Hanford and to demonstrate compliance with requirements of 10 CFR 835, the DOE Laboratory Accreditation Program, the DOE Richland Operations Office, DOE Office of River Protection, DOE Pacific Northwest Office of Science, and Hanford’s DOE contractors. The dosimetry system is operated by the Pacific Northwest National Laboratory (PNNL) Hanford External Dosimetry Program which provides dosimetry services to PNNL and all Hanford contractors. The primary users of this manual are DOE and DOE contractors at Hanford using the dosimetry services of PNNL. Development and maintenance of this manual is funded directly by DOE and DOE contractors. Its contents have been reviewed and approved by DOE and DOE contractors at Hanford through the Hanford Personnel Dosimetry Advisory Committee which is chartered and chaired by DOE-RL and serves as means of coordinating dosimetry practices across contractors at Hanford. This manual was established in 1996. Since its inception, it has been revised many times and maintained by PNNL as a controlled document with controlled distribution. The first revision to be released through PNNL’s Electronic Records & Information Capture Architecture database was designated Revision 0. Revision numbers that are whole numbers reflect major revisions typically involving significant changes to all chapters in the document. Revision

  8. Hanford External Dosimetry Technical Basis Manual PNL-MA-842

    SciTech Connect (OSTI)

    Rathbone, Bruce A.

    2010-04-01

    The Hanford External Dosimetry Technical Basis Manual PNL-MA-842 documents the design and implementation of the external dosimetry system used at the U.S. Department of Energy (DOE) Hanford site. The manual describes the dosimeter design, processing protocols, dose calculation methodology, radiation fields encountered, dosimeter response characteristics, limitations of dosimeter design under field conditions, and makes recommendations for effective use of the dosimeters in the field. The manual describes the technical basis for the dosimetry system in a manner intended to help ensure defensibility of the dose of record at Hanford and to demonstrate compliance with requirements of 10 CFR 835, the DOE Laboratory Accreditation Program, the DOE Richland Operations Office, DOE Office of River Protection, DOE Pacific Northwest Office of Science, and Hanford’s DOE contractors. The dosimetry system is operated by the Pacific Northwest National Laboratory (PNNL) Hanford External Dosimetry Program which provides dosimetry services to PNNL and all Hanford contractors. The primary users of this manual are DOE and DOE contractors at Hanford using the dosimetry services of PNNL. Development and maintenance of this manual is funded directly by DOE and DOE contractors. Its contents have been reviewed and approved by DOE and DOE contractors at Hanford through the Hanford Personnel Dosimetry Advisory Committee which is chartered and chaired by DOE-RL and serves as means of coordinating dosimetry practices across contractors at Hanford. This manual was established in 1996. Since its inception, it has been revised many times and maintained by PNNL as a controlled document with controlled distribution. The first revision to be released through PNNL’s Electronic Records & Information Capture Architecture database was designated Revision 0. Revision numbers that are whole numbers reflect major revisions typically involving significant changes to all chapters in the document. Revision

  9. Hanford External Dosimetry Technical Basis Manual PNL-MA-842

    SciTech Connect (OSTI)

    Rathbone, Bruce A.

    2007-03-12

    The Hanford External Dosimetry Technical Basis Manual PNL-MA-842 documents the design and implementation of the external dosimetry system used at Hanford. The manual describes the dosimeter design, processing protocols, dose calculation methodology, radiation fields encountered, dosimeter response characteristics, limitations of dosimeter design under field conditions, and makes recommendations for effective use of the dosimeters in the field. The manual describes the technical basis for the dosimetry system in a manner intended to help ensure defensibility of the dose of record at Hanford and to demonstrate compliance with 10 CFR 835, DOELAP, DOE-RL, ORP, PNSO, and Hanford contractor requirements. The dosimetry system is operated by PNNL’s Hanford External Dosimetry Program (HEDP) which provides dosimetry services to all Hanford contractors. The primary users of this manual are DOE and DOE contractors at Hanford using the dosimetry services of PNNL. Development and maintenance of this manual is funded directly by DOE and DOE contractors. Its contents have been reviewed and approved by DOE and DOE contractors at Hanford through the Hanford Personnel Dosimetry Advisory Committee (HPDAC) which is chartered and chaired by DOE-RL and serves as means of coordinating dosimetry practices across contractors at Hanford. This manual was established in 1996. Since inception, it has been revised many times and maintained by PNNL as a controlled document with controlled distribution. Rev. 0 marks the first revision to be released through PNNL’s Electronic Records & Information Capture Architecture (ERICA) database. Revision numbers that are whole numbers reflect major revisions typically involving changes to all chapters in the document. Revision numbers that include a decimal fraction reflect minor revisions, usually restricted to selected chapters or selected pages in the document. Revision Log: Rev. 0 (2/25/2005) Major revision and expansion. Rev. 0.1 (3/12/2007) Minor

  10. Structural basis for the antibody neutralization of Herpes simplex virus

    SciTech Connect (OSTI)

    Lee, Cheng-Chung; Lin, Li-Ling; Chan, Woan-Eng; Ko, Tzu-Ping; Lai, Jiann-Shiun; Wang, Andrew H.-J.

    2013-10-01

    The gD–E317-Fab complex crystal revealed the conformational epitope of human mAb E317 on HSV gD, providing a molecular basis for understanding the viral neutralization mechanism. Glycoprotein D (gD) of Herpes simplex virus (HSV) binds to a host cell surface receptor, which is required to trigger membrane fusion for virion entry into the host cell. gD has become a validated anti-HSV target for therapeutic antibody development. The highly inhibitory human monoclonal antibody E317 (mAb E317) was previously raised against HSV gD for viral neutralization. To understand the structural basis of antibody neutralization, crystals of the gD ectodomain bound to the E317 Fab domain were obtained. The structure of the complex reveals that E317 interacts with gD mainly through the heavy chain, which covers a large area for epitope recognition on gD, with a flexible N-terminal and C-terminal conformation. The epitope core structure maps to the external surface of gD, corresponding to the binding sites of two receptors, herpesvirus entry mediator (HVEM) and nectin-1, which mediate HSV infection. E317 directly recognizes the gD–nectin-1 interface and occludes the HVEM contact site of gD to block its binding to either receptor. The binding of E317 to gD also prohibits the formation of the N-terminal hairpin of gD for HVEM recognition. The major E317-binding site on gD overlaps with either the nectin-1-binding residues or the neutralizing antigenic sites identified thus far (Tyr38, Asp215, Arg222 and Phe223). The epitopes of gD for E317 binding are highly conserved between two types of human herpesvirus (HSV-1 and HSV-2). This study enables the virus-neutralizing epitopes to be correlated with the receptor-binding regions. The results further strengthen the previously demonstrated therapeutic and diagnostic potential of the E317 antibody.

  11. Hanford Technical Basis for Multiple Dosimetry Effective Dose Methodology

    SciTech Connect (OSTI)

    Hill, Robin L.; Rathbone, Bruce A.

    2010-08-01

    The current method at Hanford for dealing with the results from multiple dosimeters worn during non-uniform irradiation is to use a compartmentalization method to calculate the effective dose (E). The method, as documented in the current version of Section 6.9.3 in the 'Hanford External Dosimetry Technical Basis Manual, PNL-MA-842,' is based on the compartmentalization method presented in the 1997 ANSI/HPS N13.41 standard, 'Criteria for Performing Multiple Dosimetry.' With the adoption of the ICRP 60 methodology in the 2007 revision to 10 CFR 835 came changes that have a direct affect on the compartmentalization method described in the 1997 ANSI/HPS N13.41 standard, and, thus, to the method used at Hanford. The ANSI/HPS N13.41 standard committee is in the process of updating the standard, but the changes to the standard have not yet been approved. And, the drafts of the revision of the standard tend to align more with ICRP 60 than with the changes specified in the 2007 revision to 10 CFR 835. Therefore, a revised method for calculating effective dose from non-uniform external irradiation using a compartmental method was developed using the tissue weighting factors and remainder organs specified in 10 CFR 835 (2007).

  12. Development of engineering technology basis for industrialization of pyrometallurgical reprocessing

    SciTech Connect (OSTI)

    Koyama, Tadafumi; Hijikata, Takatoshi; Yokoo, Takeshi; Inoue, Tadashi

    2007-07-01

    Development of the engineering technology basis of pyrometallurgical reprocessing is a key issue for industrialization. For development of the transport technologies of molten salt and liquid cadmium at around 500 deg. C, a salt transport test rig and a metal transport test rig were installed in Ar glove box. Function of centrifugal pump and 1/2' declined tubing were confirmed with LiCl- KCl molten salt. The transport behavior of molten salt was found to follow that of water. Function of centrifugal pump, vacuum sucking and 1/2' declined tubing were confirmed with liquid Cd. With employing the transport technologies, industrialization applicable electro-refiner was newly designed and engineering-scale model was fabricated in Ar glove box. The electro-refiner has semi-continuous liquid Cd cathode instead of conventional one used in small-scale tests. With using actinide-simulating elements, demonstration of industrial-scale throughput will be carried out in this electro-refiner for more precise evaluation of industrialization potential of pyrometallurgical reprocessing. (authors)

  13. Climate Change: The Physical Basis and Latest Results

    ScienceCinema (OSTI)

    None

    2011-10-06

    The 2007 Assessment Report of the Intergovernmental Panel on Climate Change (IPCC) concludes: "Warming in the climate system is unequivocal." Without the contribution of Physics to climate science over many decades, such a statement would not have been possible. Experimental physics enables us to read climate archives such as polar ice cores and so provides the context for the current changes. For example, today the concentration of CO2 in the atmosphere, the second most important greenhouse gas, is 28% higher than any time during the last 800,000 years. Classical fluid mechanics and numerical mathematics are the basis of climate models from which estimates of future climate change are obtained. But major instabilities and surprises in the Earth System are still unknown. These are also to be considered when the climatic consequences of proposals for geo-engineering are estimated. Only Physics will permit us to further improve our understanding in order to provide the foundation for policy decisions facing the global climate change challenge.

  14. Hanford External Dosimetry Technical Basis Manual PNL-MA-842

    SciTech Connect (OSTI)

    Rathbone, Bruce A.

    2010-01-01

    The Hanford External Dosimetry Technical Basis Manual PNL-MA-842 documents the design and implementation of the external dosimetry system used at Hanford. The manual describes the dosimeter design, processing protocols, dose calculation methodology, radiation fields encountered, dosimeter response characteristics, limitations of dosimeter design under field conditions, and makes recommendations for effective use of the dosimeters in the field. The manual describes the technical basis for the dosimetry system in a manner intended to help ensure defensibility of the dose of record at Hanford and to demonstrate compliance with 10 CFR 835, DOELAP, DOE-RL, ORP, PNSO, and Hanford contractor requirements. The dosimetry system is operated by PNNL’s Hanford External Dosimetry Program (HEDP) which provides dosimetry services to all Hanford contractors. The primary users of this manual are DOE and DOE contractors at Hanford using the dosimetry services of PNNL. Development and maintenance of this manual is funded directly by DOE and DOE contractors. Its contents have been reviewed and approved by DOE and DOE contractors at Hanford through the Hanford Personnel Dosimetry Advisory Committee (HPDAC) which is chartered and chaired by DOE-RL and serves as means of coordinating dosimetry practices across contractors at Hanford. This manual was established in 1996. Since its inception, it has been revised many times and maintained by PNNL as a controlled document with controlled distribution. The first revision to be released through PNNL’s Electronic Records & Information Capture Architecture (ERICA) database was designated Revision 0. Revision numbers that are whole numbers reflect major revisions typically involving significant changes to all chapters in the document. Revision numbers that include a decimal fraction reflect minor revisions, usually restricted to selected chapters or selected pages in the document. Maintenance and distribution of controlled hard copies of the

  15. Safety evaluation of MHTGR licensing basis accident scenarios

    SciTech Connect (OSTI)

    Kroeger, P.G.

    1989-04-01

    The safety potential of the Modular High-Temperature Gas Reactor (MHTGR) was evaluated, based on the Preliminary Safety Information Document (PSID), as submitted by the US Department of Energy to the US Nuclear Regulatory Commission. The relevant reactor safety codes were extended for this purpose and applied to this new reactor concept, searching primarily for potential accident scenarios that might lead to fuel failures due to excessive core temperatures and/or to vessel damage, due to excessive vessel temperatures. The design basis accident scenario leading to the highest vessel temperatures is the depressurized core heatup scenario without any forced cooling and with decay heat rejection to the passive Reactor Cavity Cooling System (RCCS). This scenario was evaluated, including numerous parametric variations of input parameters, like material properties and decay heat. It was found that significant safety margins exist, but that high confidence levels in the core effective thermal conductivity, the reactor vessel and RCCS thermal emissivities and the decay heat function are required to maintain this safety margin. Severe accident extensions of this depressurized core heatup scenario included the cases of complete RCCS failure, cases of massive air ingress, core heatup without scram and cases of degraded RCCS performance due to absorbing gases in the reactor cavity. Except for no-scram scenarios extending beyond 100 hr, the fuel never reached the limiting temperature of 1600/degree/C, below which measurable fuel failures are not expected. In some of the scenarios, excessive vessel and concrete temperatures could lead to investment losses but are not expected to lead to any source term beyond that from the circulating inventory. 19 refs., 56 figs., 11 tabs.

  16. Practical auxiliary basis implementation of Rung 3.5 functionals

    SciTech Connect (OSTI)

    Janesko, Benjamin G.; Scalmani, Giovanni; Frisch, Michael J.

    2014-07-21

    Approximate exchange-correlation functionals for Kohn-Sham density functional theory often benefit from incorporating exact exchange. Exact exchange is constructed from the noninteracting reference system's nonlocal one-particle density matrix γ(r{sup -vector},r{sup -vector}′). Rung 3.5 functionals attempt to balance the strengths and limitations of exact exchange using a new ingredient, a projection of γ(r{sup -vector},r{sup -vector} ′) onto a semilocal model density matrix γ{sub SL}(ρ(r{sup -vector}),∇ρ(r{sup -vector}),r{sup -vector}−r{sup -vector} ′). γ{sub SL} depends on the electron density ρ(r{sup -vector}) at reference point r{sup -vector}, and is closely related to semilocal model exchange holes. We present a practical implementation of Rung 3.5 functionals, expanding the r{sup -vector}−r{sup -vector} ′ dependence of γ{sub SL} in an auxiliary basis set. Energies and energy derivatives are obtained from 3D numerical integration as in standard semilocal functionals. We also present numerical tests of a range of properties, including molecular thermochemistry and kinetics, geometries and vibrational frequencies, and bandgaps and excitation energies. Rung 3.5 functionals typically provide accuracy intermediate between semilocal and hybrid approximations. Nonlocal potential contributions from γ{sub SL} yield interesting successes and failures for band structures and excitation energies. The results enable and motivate continued exploration of Rung 3.5 functional forms.

  17. Structural Basis of Selective Ubiquitination of TRF1 by SCFFbx4...

    Office of Scientific and Technical Information (OSTI)

    Structural Basis of Selective Ubiquitination of TRF1 by SCFFbx4 Citation Details In-Document Search Title: Structural Basis of Selective Ubiquitination of TRF1 by SCFFbx4 Authors: ...

  18. Structural basis for Notch1 engagement of Delta-like 4 (Journal...

    Office of Scientific and Technical Information (OSTI)

    Structural basis for Notch1 engagement of Delta-like 4 Citation Details In-Document Search Title: Structural basis for Notch1 engagement of Delta-like 4 Authors: Luca, Vincent C. ; ...

  19. A new paradigm for the molecular basis of rubber elasticity

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hanson, David E.; Barber, John L.

    2015-02-19

    The molecular basis for rubber elasticity is arguably the oldest and one of the most important questions in the field of polymer physics. The theoretical investigation of rubber elasticity began in earnest almost a century ago with the development of analytic thermodynamic models, based on simple, highly-symmetric configurations of so-called Gaussian chains, i.e. polymer chains that obey Markov statistics. Numerous theories have been proposed over the past 90 years based on the ansatz that the elastic force for individual network chains arises from the entropy change associated with the distribution of end-to-end distances of a free polymer chain. There aremore » serious philosophical objections to this assumption and others, such as the assumption that all network nodes undergo affine motion and that all of the network chains have the same length. Recently, a new paradigm for elasticity in rubber networks has been proposed that is based on mechanisms that originate at the molecular level. Using conventional statistical mechanics analyses, quantum chemistry, and molecular dynamics simulations, the fundamental entropic and enthalpic chain extension forces for polyisoprene (natural rubber) have been determined, along with estimates for the basic force constants. Concurrently, the complex morphology of natural rubber networks (the joint probability density distributions that relate the chain end-to-end distance to its contour length) has also been captured in a numerical model. When molecular chain forces are merged with the network structure in this model, it is possible to study the mechanical response to tensile and compressive strains of a representative volume element of a polymer network. As strain is imposed on a network, pathways of connected taut chains, that completely span the network along strain axis, emerge. Although these chains represent only a few percent of the total, they account for nearly all of the elastic stress at high strain. Here we provide

  20. TECHNICAL BASIS FOR VENTILATION REQUIREMENTS IN TANK FARMS OPERATING SPECIFICATIONS DOCUMENTS

    SciTech Connect (OSTI)

    BERGLIN, E J

    2003-06-23

    This report provides the technical basis for high efficiency particulate air filter (HEPA) for Hanford tank farm ventilation systems (sometimes known as heating, ventilation and air conditioning [HVAC]) to support limits defined in Process Engineering Operating Specification Documents (OSDs). This technical basis included a review of older technical basis and provides clarifications, as necessary, to technical basis limit revisions or justification. This document provides an updated technical basis for tank farm ventilation systems related to Operation Specification Documents (OSDs) for double-shell tanks (DSTs), single-shell tanks (SSTs), double-contained receiver tanks (DCRTs), catch tanks, and various other miscellaneous facilities.

  1. CRITICALITY SAFETY CONTROLS AND THE SAFETY BASIS AT PFP

    SciTech Connect (OSTI)

    Kessler, S

    2009-04-21

    reviewing documents used in classifying controls for Nuclear Safety, it was noted that DOE-HDBK-1188, 'Glossary of Environment, Health, and Safety Terms', defines an Administrative Control (AC) in terms that are different than typically used in Criticality Safety. As part of this CCR, a new term, Criticality Administrative Control (CAC) was defined to clarify the difference between an AC used for criticality safety and an AC used for nuclear safety. In Nuclear Safety terms, an AC is a provision relating to organization and management, procedures, recordkeeping, assessment, and reporting necessary to ensure safe operation of a facility. A CAC was defined as an administrative control derived in a criticality safety analysis that is implemented to ensure double contingency. According to criterion 2 of Section IV, 'Linkage to the Documented Safety Analysis', of DOESTD-3007-2007, the consequence of a criticality should be examined for the purposes of classifying the significance of a control or component. HNF-PRO-700, 'Safety Basis Development', provides control selection criteria based on consequence and risk that may be used in the development of a Criticality Safety Evaluation (CSE) to establish the classification of a component as a design feature, as safety class or safety significant, i.e., an Engineered Safety Feature (ESF), or as equipment important to safety; or merely provides defense-in-depth. Similar logic is applied to the CACs. Criterion 8C of DOE-STD-3007-2007, as written, added to the confusion of using the basic CCR from HNF-7098. The PFP CCR attempts to clarify this criterion by revising it to say 'Programmatic commitments or general references to control philosophy (e.g., mass control or spacing control or concentration control as an overall control strategy for the process without specific quantification of individual limits) is included in the PFP DSA'. Table 1 shows the PFP methodology for evaluating CACs. This evaluation process has been in use since

  2. Hydroetching of high surface area ceramics using moist supercritical fluids

    DOE Patents [OSTI]

    Fryxell, Glen; Zemanian, Thomas S.

    2004-11-02

    Aerogels having a high density of hydroxyl groups and a more uniform pore size with fewer bottlenecks are described. The aerogel is exposed to a mixture of a supercritical fluid and water, whereupon the aerogel forms a high density of hydroxyl groups. The process also relaxes the aerogel into a more open uniform internal structure, in a process referred to as hydroetching. The hydroetching process removes bottlenecks from the aerogels, and forms the hydrogels into more standard pore sizes while preserving their high surface area.

  3. Enterprise Assessments Targeted Review of the Safety Basis at the Savannah

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    River Site F-Area Central Laboratory Facility - January 2016 | Department of Energy Basis at the Savannah River Site F-Area Central Laboratory Facility - January 2016 Enterprise Assessments Targeted Review of the Safety Basis at the Savannah River Site F-Area Central Laboratory Facility - January 2016 January 2016 Review of the Safety Basis F-Area Central Laboratory Facility at the Savannah River Site The Office of Nuclear Safety and Environmental Assessments, within the U.S. Department of

  4. 2010 DOE National Science Bowl® Photos - Basis Charter School...

    Office of Science (SC) Website

    Basis Charter School National Science Bowl (NSB) NSB Home About National Science Bowl Contacts Regional Science Bowl Coordinators National Science Bowl FAQ's Alumni Past National ...

  5. Technical Basis and Considerations for DOE M 435.1-1 (Appendix A)

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1999-07-09

    This appendix establishes the technical basis of the order revision process and of each of the requirements included in the revised radioactive waste management order.

  6. A Brief Review of the Basis for, and the Procedures Currently Utilized in,

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Gross Gamma-Ray Log Calibration (October 1976) | Department of Energy A Brief Review of the Basis for, and the Procedures Currently Utilized in, Gross Gamma-Ray Log Calibration (October 1976) A Brief Review of the Basis for, and the Procedures Currently Utilized in, Gross Gamma-Ray Log Calibration (October 1976) A Brief Review of the Basis for, and the Procedures Currently Utilized in, Gross Gamma-Ray Log Calibration (October 1976) A Brief Review of the Basis for, and the Procedures

  7. Report to the Secretary of Energy on Beyond Design Basis Event...

    Broader source: Energy.gov (indexed) [DOE]

    BDBEReportfinal.pdf More Documents & Publications Report to the Secretary of Energy on Beyond Design Basis Event Pilot Evaluations, Results and Recommendations for Improvements...

  8. Structural basis for the prion-like MAVS filaments in antiviral...

    Office of Scientific and Technical Information (OSTI)

    in antiviral innate immunity Citation Details In-Document Search Title: Structural basis for the prion-like MAVS filaments in antiviral innate immunity Authors: Xu, Hui ; He, ...

  9. CRAD, Review of Safety Basis Development - May 6, 2013 | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Review of Safety Basis Development - May 6, 2013 CRAD, Review of Safety Basis Development - May 6, 2013 May 6, 2013 Review of Safety Basis Development for the Los Alamos National Laboratory Transuranic Waste Facility (HSS CRAD 45-59, Rev. 0) The review will consider selected aspects of the development of safety basis for the Transuranic Waste Facility (TWF) to assess the extent to which safety is integrated into the design of the TWF in accordance with DOE directives; in particular,

  10. Engineering basis for operator control of nuclear power stations in abnormal operations - closing the loop

    SciTech Connect (OSTI)

    Womack, E.A.; Kelly, J.J.; Elliott, N.S.

    1980-01-01

    The Abnormal Transient Operating Guidelines (ATOG) Program is intended to ''close the loop'' on a continuing basis between the engineering designers/performance analysts and the operators who control the plant. It will make the technical basis for operation responsive to information from the study of actual plant transients, as well as new developments in engineering.

  11. Establishing the Technical Basis for Disposal of Heat-generating Waste in Salt

    Broader source: Energy.gov [DOE]

    The report summarizes available historic tests and the developed technical basis for disposal of heat-generating waste in salt, and the means by which a safety case for disposal of heat generating waste at a generic salt site can be initiated from the existing technical basis.

  12. Criteria Document for B-plant Surveillance and Maintenance Phase Safety Basis Document

    SciTech Connect (OSTI)

    SCHWEHR, B.A.

    1999-08-31

    This document is required by the Project Hanford Managing Contractor (PHMC) procedure, HNF-PRO-705, Safety Basis Planning, Documentation, Review, and Approval. This document specifies the criteria that shall be in the B Plant surveillance and maintenance phase safety basis in order to obtain approval of the DOE-RL. This CD describes the criteria to be addressed in the S&M Phase safety basis for the deactivated Waste Fractionization Facility (B Plant) on the Hanford Site in Washington state. This criteria document describes: the document type and format that will be used for the S&M Phase safety basis, the requirements documents that will be invoked for the document development, the deactivated condition of the B Plant facility, and the scope of issues to be addressed in the S&M Phase safety basis document.

  13. Safety basis academy summary of project implementation from 2007-2009

    SciTech Connect (OSTI)

    Johnston, Julie A

    2009-01-01

    During fiscal years 2007 through 2009, in accordance with Performance Based Incentives with DOE/NNSA Los Alamos Site Office, Los Alamos National Security (LANS) implemented and operated a Safety Basis Academy (SBA) to facilitate uniformity in technical qualifications of safety basis professionals across the nuclear weapons complex. The implementation phase of the Safety Basis Academy required development, delivery, and finalizing a set of 23 courses. The courses developed are capable of supporting qualification efforts for both federal and contractor personnel throughout the DOE/NNSA Complex. The LANS Associate Director for Nuclear and High Hazard Operations (AD-NHHO) delegated project responsibillity to the Safety Basis Division. The project was assigned to the Safety Basis Technical Services (SB-TS) Group at Los Alamos National Laboratory (LANL). The main tasks were project needs analysis, design, development, implementation of instructional delivery, and evaluation of SBA courses. DOE/NNSA responsibility for oversight of the SBA project was assigned to the Chief of Defense for Nuclear Safety, and delegated to the Authorization Basis Senior Advisor, Continuous Learning Chair (CDNS-ABSA/CLC). NNSA developed a memorandum of agreement with LANS AD-NHHO. Through a memorandum of agreement initiated by NNSA, the DOE National Training Center (NTC) will maintain the set of Safety Basis Academy courses and is able to facilitate course delivery throughout the DOE Complex.

  14. Using an iterative eigensolver to compute vibrational energies with phase-spaced localized basis functions

    SciTech Connect (OSTI)

    Brown, James Carrington, Tucker

    2015-07-28

    Although phase-space localized Gaussians are themselves poor basis functions, they can be used to effectively contract a discrete variable representation basis [A. Shimshovitz and D. J. Tannor, Phys. Rev. Lett. 109, 070402 (2012)]. This works despite the fact that elements of the Hamiltonian and overlap matrices labelled by discarded Gaussians are not small. By formulating the matrix problem as a regular (i.e., not a generalized) matrix eigenvalue problem, we show that it is possible to use an iterative eigensolver to compute vibrational energy levels in the Gaussian basis.

  15. Structural Basis of UV DNA-Damage Recognition by the DDB1-DDB2...

    Office of Scientific and Technical Information (OSTI)

    Recognition by the DDB1-DDB2 Complex Citation Details In-Document Search Title: Structural Basis of UV DNA-Damage Recognition by the DDB1-DDB2 Complex Ultraviolet (UV) ...

  16. NSS 18.3 Verification of Authorization Basis Documentation 12/8/03

    Broader source: Energy.gov [DOE]

    The objective of this surveillance is for the Facility Representative to verify that the facility's configuration and operations remain consistent with the authorization basis.  As defined in DOE...

  17. Reducing Uncertainty in the Seismic Design Basis for the Waste Treatment Plant, Hanford, Washington

    SciTech Connect (OSTI)

    Brouns, Thomas M.; Rohay, Alan C.; Reidel, Steve; Gardner, Martin G.

    2007-02-27

    The seismic design basis for the Waste Treatment Plant (WTP) at the Department of Energys (DOE) Hanford Site near Richland was re-evaluated in 2005, resulting in an increase by up to 40% in the seismic design basis. The original seismic design basis for the WTP was established in 1999 based on a probabilistic seismic hazard analysis completed in 1996. The 2005 analysis was performed to address questions raised by the Defense Nuclear Facilities Safety Board (DNFSB) about the assumptions used in developing the original seismic criteria and adequacy of the site geotechnical surveys. The updated seismic response analysis used existing and newly acquired seismic velocity data, statistical analysis, expert elicitation, and ground motion simulation to develop interim design ground motion response spectra which enveloped the remaining uncertainties. The uncertainties in these response spectra were enveloped at approximately the 84th percentile to produce conservative design spectra, which contributed significantly to the increase in the seismic design basis.

  18. Structure of P-Glycoprotein Reveals a Molecular Basis for Poly...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Structure of P-Glycoprotein Reveals a Molecular Basis for Poly-Specific Drug Binding figure 1 Figure 1. Structure of P-gp. Many forms of cancer fail to respond to chemotherapy by ...

  19. Technical Basis Spent Nuclear Fuel (SNF) Project Radiation and Contamination Trending Program

    SciTech Connect (OSTI)

    KURTZ, J.E.

    2000-05-10

    This report documents the technical basis for the Spent Nuclear Fuel (SNF) Program radiation and contamination trending program. The program consists of standardized radiation and contamination surveys of the KE Basin, radiation surveys of the KW basin, and radiation surveys of the Cold Vacuum Drying Facility (CVD) with the associated tracking. This report also discusses the remainder of radiological areas within the SNFP that do not have standardized trending programs and the basis for not having this program in those areas.

  20. Bound state calculations in QED and QCD using basis light-front

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    quantization | Argonne Leadership Computing Facility Bound state calculations in QED and QCD using basis light-front quantization Authors: Pieter Maris, Paul Wiecki, Yang Li, Xingbo Zhao, James P. Vary In order to describe self-bound systems, one needs a nonperturbative approach. We discuss the relativistic bound state equations of QED and QCD formulated in Basis Light-Front Quantization. In this approach, the light-front direction is discretized, and two-dimensional harmonic oscillator

  1. DOE's Safety Bulletin No. 2011-01, Events Beyond Design Safety Basis

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Analysis, March 2011 | Department of Energy DOE's Safety Bulletin No. 2011-01, Events Beyond Design Safety Basis Analysis, March 2011 DOE's Safety Bulletin No. 2011-01, Events Beyond Design Safety Basis Analysis, March 2011 PURPOSE This Safety Alert provides information on a safety concern related to the identification and mitigation of events that may fall outside those analyzed in the documented safety analysis. BACKGROUND On March 11, 2011, the Fukushima Daiichi nuclear power station in

  2. Non-homogeneous solutions of a Coulomb Schrdinger equation as basis set for scattering problems

    SciTech Connect (OSTI)

    Del Punta, J. A.; Ambrosio, M. J.; Gasaneo, G.; Zaytsev, S. A.; Ancarani, L. U.

    2014-05-15

    We introduce and study two-body Quasi Sturmian functions which are proposed as basis functions for applications in three-body scattering problems. They are solutions of a two-body non-homogeneous Schrdinger equation. We present different analytic expressions, including asymptotic behaviors, for the pure Coulomb potential with a driven term involving either Slater-type or Laguerre-type orbitals. The efficiency of Quasi Sturmian functions as basis set is numerically illustrated through a two-body scattering problem.

  3. The power of simplification: Operator interface with the AP1000{sup R} during design-basis and beyond design-basis events

    SciTech Connect (OSTI)

    Williams, M. G.; Mouser, M. R.; Simon, J. B.

    2012-07-01

    The AP1000{sup R} plant is an 1100-MWe pressurized water reactor with passive safety features and extensive plant simplifications that enhance construction, operation, maintenance, safety and cost. The passive safety features are designed to function without safety-grade support systems such as component cooling water, service water, compressed air or HVAC. The AP1000 passive safety features achieve and maintain safe shutdown in case of a design-basis accident for 72 hours without need for operator action, meeting the expectations provided in the European Utility Requirements and the Utility Requirement Document for passive plants. Limited operator actions may be required to maintain safe conditions in the spent fuel pool (SFP) via passive means. This safety approach therefore minimizes the reliance on operator action for accident mitigation, and this paper examines the operator interaction with the Human-System Interface (HSI) as the severity of an accident increases from an anticipated transient to a design basis accident and finally, to a beyond-design-basis event. The AP1000 Control Room design provides an extremely effective environment for addressing the first 72 hours of design-basis events and transients, providing ease of information dissemination and minimal reliance upon operator actions. Symptom-based procedures including Emergency Operating Procedures (EOPs), Abnormal Operating Procedures (AOPs) and Alarm Response Procedures (ARPs) are used to mitigate design basis transients and accidents. Use of the Computerized Procedure System (CPS) aids the operators during mitigation of the event. The CPS provides cues and direction to the operators as the event progresses. If the event becomes progressively worse or lasts longer than 72 hours, and depending upon the nature of failures that may have occurred, minimal operator actions may be required outside of the control room in areas that have been designed to be accessible using components that have been

  4. Hamiltonian Light-Front Ffield Theory in a Basis Function Approach

    SciTech Connect (OSTI)

    Vary, J.P.; Honkanen, H.; Li, Jun; Maris, P.; Brodsky, S.J.; Harindranath, A.; de Teramond, G.F.; Sternberg, P.; Ng, E.G.; Yang, C.

    2009-05-15

    Hamiltonian light-front quantum field theory constitutes a framework for the non-perturbative solution of invariant masses and correlated parton amplitudes of self-bound systems. By choosing the light-front gauge and adopting a basis function representation, we obtain a large, sparse, Hamiltonian matrix for mass eigenstates of gauge theories that is solvable by adapting the ab initio no-core methods of nuclear many-body theory. Full covariance is recovered in the continuum limit, the infinite matrix limit. There is considerable freedom in the choice of the orthonormal and complete set of basis functions with convenience and convergence rates providing key considerations. Here, we use a two-dimensional harmonic oscillator basis for transverse modes that corresponds with eigensolutions of the soft-wall AdS/QCD model obtained from light-front holography. We outline our approach, present illustrative features of some non-interacting systems in a cavity and discuss the computational challenges.

  5. Time-dependent density functional theory quantum transport simulation in non-orthogonal basis

    SciTech Connect (OSTI)

    Kwok, Yan Ho; Xie, Hang; Yam, Chi Yung; Chen, Guan Hua; Zheng, Xiao

    2013-12-14

    Basing on the earlier works on the hierarchical equations of motion for quantum transport, we present in this paper a first principles scheme for time-dependent quantum transport by combining time-dependent density functional theory (TDDFT) and Keldysh's non-equilibrium Green's function formalism. This scheme is beyond the wide band limit approximation and is directly applicable to the case of non-orthogonal basis without the need of basis transformation. The overlap between the basis in the lead and the device region is treated properly by including it in the self-energy and it can be shown that this approach is equivalent to a lead-device orthogonalization. This scheme has been implemented at both TDDFT and density functional tight-binding level. Simulation results are presented to demonstrate our method and comparison with wide band limit approximation is made. Finally, the sparsity of the matrices and computational complexity of this method are analyzed.

  6. Electron Anomalous Magnetic Moment in Basis Light-Front Quantization Approach

    SciTech Connect (OSTI)

    Zhao, Xingbo; Honkanen, Heli; Maris, Pieter; Vary, James P.; Brodsky, Stanley J.; /SLAC

    2012-02-17

    We apply the Basis Light-Front Quantization (BLFQ) approach to the Hamiltonian field theory of Quantum Electrodynamics (QED) in free space. We solve for the mass eigenstates corresponding to an electron interacting with a single photon in light-front gauge. Based on the resulting non-perturbative ground state light-front amplitude we evaluate the electron anomalous magnetic moment. The numerical results from extrapolating to the infinite basis limit reproduce the perturbative Schwinger result with relative deviation less than 1.2%. We report significant improvements over previous works including the development of analytic methods for evaluating the vertex matrix elements of QED.

  7. Technical Basis Spent Nuclear Fuel (SNF) Project Radiation and Contamination Trending Program

    SciTech Connect (OSTI)

    ELGIN, J.C.

    2000-10-02

    This report documents the technical basis for the Spent Nuclear Fuel (SNF) Program radiation and contamination trending program. The program consists of standardized radiation and contamination surveys of the KE Basin, radiation surveys of the KW basin, radiation surveys of the Cold Vacuum Drying Facility (CVD), and radiation surveys of the Canister Storage Building (CSB) with the associated tracking. This report also discusses the remainder of radiological areas within the SNFP that do not have standardized trending programs and the basis for not having this program in those areas.

  8. Universal basis of two-center functions. Test computations of certain diatomic molecules and ions

    SciTech Connect (OSTI)

    Kirnos, V.F.; Samsonov, B.F.; Cheglokov, E.I.

    1987-05-01

    It is shown that the basis of two-center functions is universal. The dependence of the nuclei of atoms comprising a molecule on charges and on the intranuclear spacing is separated explicitly in the integrals used in analyzing diatomic molecules. The basis integrals constructed once permitted rapid and effective execution of computations for the ground state potential curves for a number of electron systems: H/sub 2/, He/sub 2//sup 2 +/, HeH/sup +/, He/sub 2/, LiH, Li/sub 2/, HeB/sup +/, Be/sub 2/.

  9. Analytic matrix elements for the two-electron atomic basis with logarithmic terms

    SciTech Connect (OSTI)

    Liverts, Evgeny Z.; Barnea, Nir

    2014-08-01

    The two-electron problem for the helium-like atoms in S-state is considered. The basis containing the integer powers of ln r, where r is a radial variable of the Fock expansion, is studied. In this basis, the analytic expressions for the matrix elements of the corresponding Hamiltonian are presented. These expressions include only elementary and special functions, what enables very fast and accurate computation of the matrix elements. The decisive contribution of the correct logarithmic terms to the behavior of the two-electron wave function in the vicinity of the triple-coalescence point is reaffirmed.

  10. Design-Basis Flood Estimation for Site Characterization at Nuclear Power Plants in the United States of America

    SciTech Connect (OSTI)

    Prasad, Rajiv; Hibler, Lyle F.; Coleman, Andre M.; Ward, Duane L.

    2011-11-01

    The purpose of this document is to describe approaches and methods for estimation of the design-basis flood at nuclear power plant sites. Chapter 1 defines the design-basis flood and lists the U.S. Nuclear Regulatory Commission's (NRC) regulations that require estimation of the design-basis flood. For comparison, the design-basis flood estimation methods used by other Federal agencies are also described. A brief discussion of the recommendations of the International Atomic Energy Agency for estimation of the design-basis floods in its member States is also included.

  11. Margin of Safety Definition and Examples Used in Safety Basis Documents and the USQ Process

    SciTech Connect (OSTI)

    Beaulieu, R. A.

    2013-10-03

    The Nuclear Safety Management final rule, 10 CFR 830, provides an undefined term, margin of safety (MOS). Safe harbors listed in 10 CFR 830, Table 2, such as DOE-STD-3009 use but do not define the term. This lack of definition has created the need for the definition. This paper provides a definition of MOS and documents examples of MOS as applied in a U.S. Department of Energy (DOE) approved safety basis for an existing nuclear facility. If we understand what MOS looks like regarding Technical Safety Requirements (TSR) parameters, then it helps us compare against other parameters that do not involve a MOS. This paper also documents parameters that are not MOS. These criteria could be used to determine if an MOS exists in safety basis documents. This paper helps DOE, including the National Nuclear Security Administration (NNSA) and its contractors responsible for the safety basis improve safety basis documents and the unreviewed safety question (USQ) process with respect to MOS.

  12. CRAD, Safety Basis- Oak Ridge National Laboratory High Flux Isotope Reactor

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for a February 2007 assessment of the Safety Basis in preparation for restart of the Oak Ridge National Laboratory High Flux Isotope Reactor.

  13. CRAD, Safety Basis- Oak Ridge National Laboratory High Flux Isotope Reactor Contractor ORR

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for a February 2007 assessment of the Safety Basis portion of an Operational Readiness Review of the Oak Ridge National Laboratory High Flux Isotope Reactor.

  14. Preliminary tank characterization report for single-shell tank 241-A-104: best basis inventory

    SciTech Connect (OSTI)

    Hodgson, K.M.

    1997-07-01

    An effort is underway to provide waste inventory estimates that will serve as standard characterization source terms for the various waste management activities. As part of this effort, an evaluation of available information for single-shell tank 241-A-104 was performed, and a best-basis inventory was established. This work follows the methodology that was established by the standard inventory task.

  15. CRAD, Safety Basis- Oak Ridge National Laboratory TRU ALPHA LLWT Project

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for a November 2003 assessment of the Safety Basis portion of an Operational Readiness Review of the Oak Ridge National Laboratory TRU ALPHA LLWT Project.

  16. The Haldane–Shastry spin chain and the topological basis realization

    SciTech Connect (OSTI)

    Sun, Chunfang; Gou, Lidan; Wang, Gangcheng; Du, Guijiao; Zhou, Chengcheng; Xue, Kang

    2013-06-15

    In this paper, based on the topological basis states, we investigate the Hamiltonian family (H{sub 2},H{sub 3},H{sub 4}) of a closed four-qubit Haldane–Shastry spin chain. Not only the two-qubit interaction form, but also the three-qubit interaction form and the four-qubit interaction form are presented in terms of spin operators. Meanwhile, we explore some particular properties of the topological basis states in these systems. With Yangian algebra, the symmetry of the systems and the transitions between the eigenstates have been investigated. We find a really useful effect of Y(sl(2)) operators (J{sub ±},J{sub 3}), which is that they can describe the transitions between the spin single state and the spin triple states. Furthermore, we construct a new Hamiltonian, whose energy degeneracies can be changed by adjusting the strengths of the two-qubit interactions, three-qubit interactions, four-qubit interactions, and the external magnetic field. -- Highlights: •We study the Haldane–Shastry model based on the topological basis realization. •Really useful effects of Yangian operators are found. •We explore some particular properties of the topological basis in these systems. •We construct a new Hamiltonian whose energy degeneracy can be changed.

  17. Dynamical properties of non-ideal plasma on the basis of effective potentials

    SciTech Connect (OSTI)

    Ramazanov, T. S.; Kodanova, S. K.; Moldabekov, Zh. A.; Issanova, M. K.

    2013-11-15

    In this work, stopping power has been calculated on the basis of the Coulomb logarithm using the effective potentials. Calculations of the Coulomb logarithm and stopping power for different interaction potentials and degrees of ionization are compared. The comparison with the data of other theoretical and experimental works was carried out.

  18. Sensitivity of the Properties of Ruthenium Blue Dimer to Method, Basis Set, and Continuum Model

    SciTech Connect (OSTI)

    Ozkanlar, Abdullah; Clark, Aurora E.

    2012-05-23

    The ruthenium blue dimer [(bpy)2RuIIIOH2]2O4+ is best known as the first well-defined molecular catalyst for water oxidation. It has been subject to numerous computational studies primarily employing density functional theory. However, those studies have been limited in the functionals, basis sets, and continuum models employed. The controversy in the calculated electronic structure and the reaction energetics of this catalyst highlights the necessity of benchmark calculations that explore the role of density functionals, basis sets, and continuum models upon the essential features of blue-dimer reactivity. In this paper, we report Kohn-Sham complete basis set (KS-CBS) limit extrapolations of the electronic structure of blue dimer using GGA (BPW91 and BP86), hybrid-GGA (B3LYP), and meta-GGA (M06-L) density functionals. The dependence of solvation free energy corrections on the different cavity types (UFF, UA0, UAHF, UAKS, Bondi, and Pauling) within polarizable and conductor-like polarizable continuum model has also been investigated. The most common basis sets of double-zeta quality are shown to yield results close to the KS-CBS limit; however, large variations are observed in the reaction energetics as a function of density functional and continuum cavity model employed.

  19. CRAD, Safety Basis- Y-12 Enriched Uranium Operations Oxide Conversion Facility

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for a January 2005 assessment of the Safety Basis at the Y-12 - Enriched Uranium Operations Oxide Conversion Facility.

  20. CRAD, Safety Basis- Los Alamos National Laboratory TA 55 SST Facility

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for an assessment of the Safety Basis at the Los Alamos National Laboratory TA 55 SST Facility.

  1. Technical basis for the aboveground structure failure and associated represented hazardous conditions

    SciTech Connect (OSTI)

    MANGAN, D.

    2003-03-20

    The purpose of the Technical Basis Document is to determine the consequences and frequency of aboveground structure failures. These failures include drops of contained equipment, such as a pump, from a SST or DST, a crane failure resulting in a load drop onto a HEPA filter. These failures can result in an uncontrolled release of radiological and toxicological material.

  2. Tank Waste Remediation System (TWRS) Retrieval Authorization Basis Amendment Task Plan

    SciTech Connect (OSTI)

    HARRIS, J.P.

    1999-08-31

    This task plan is a documented agreement between Nuclear Safety and Licensing and Retrieval Engineering. The purpose of this task plan is to identify the scope of work, tasks and deliverables, responsibilities, manpower, and schedules associated with an authorization basis amendment as a result of the Waste Feed Delivery Program, Project W-211, Project W-521, and Project W-522.

  3. Tank Waste Remediation System (TWRS) Retrieval Authorization Basis Amendment Task Plan

    SciTech Connect (OSTI)

    HARRIS, J.P.

    2000-03-27

    This task plan is a documented agreement between Nuclear Safety and Licensing and Retrieval Engineering. The purpose of this task plan is to identify the scope of work, tasks and deliverables, responsibilities, manpower, and schedules associated with an authorization basis amendment as a result of the Waste Feed Delivery Program, Project W-211, Project W-521, and Project W-522.

  4. CRAD, Safety Basis- Los Alamos National Laboratory Waste Characterization, Reduction, and Repackaging Facility

    Broader source: Energy.gov [DOE]

    A section of Appendix C to DOE G 226.1-2 "Federal Line Management Oversight of Department of Energy Nuclear Facilities." Consists of Criteria Review and Approach Documents (CRADs) used for an assessment of the Safety Basis portion of an Operational Readiness Review at the Los Alamos National Laboratory Waste Characterization, Reduction, and Repackaging Facility.

  5. Correlation consistent basis sets for actinides. I. The Th and U atoms

    SciTech Connect (OSTI)

    Peterson, Kirk A.

    2015-02-21

    New correlation consistent basis sets based on both pseudopotential (PP) and all-electron Douglas-Kroll-Hess (DKH) Hamiltonians have been developed from double- to quadruple-zeta quality for the actinide atoms thorium and uranium. Sets for valence electron correlation (5f6s6p6d), cc − pV nZ − PP and cc − pV nZ − DK3, as well as outer-core correlation (valence + 5s5p5d), cc − pwCV nZ − PP and cc − pwCV nZ − DK3, are reported (n = D, T, Q). The -PP sets are constructed in conjunction with small-core, 60-electron PPs, while the -DK3 sets utilized the 3rd-order Douglas-Kroll-Hess scalar relativistic Hamiltonian. Both series of basis sets show systematic convergence towards the complete basis set limit, both at the Hartree-Fock and correlated levels of theory, making them amenable to standard basis set extrapolation techniques. To assess the utility of the new basis sets, extensive coupled cluster composite thermochemistry calculations of ThF{sub n} (n = 2 − 4), ThO{sub 2}, and UF{sub n} (n = 4 − 6) have been carried out. After accurately accounting for valence and outer-core correlation, spin-orbit coupling, and even Lamb shift effects, the final 298 K atomization enthalpies of ThF{sub 4}, ThF{sub 3}, ThF{sub 2}, and ThO{sub 2} are all within their experimental uncertainties. Bond dissociation energies of ThF{sub 4} and ThF{sub 3}, as well as UF{sub 6} and UF{sub 5}, were similarly accurate. The derived enthalpies of formation for these species also showed a very satisfactory agreement with experiment, demonstrating that the new basis sets allow for the use of accurate composite schemes just as in molecular systems composed only of lighter atoms. The differences between the PP and DK3 approaches were found to increase with the change in formal oxidation state on the actinide atom, approaching 5-6 kcal/mol for the atomization enthalpies of ThF{sub 4} and ThO{sub 2}. The DKH3 atomization energy of ThO{sub 2} was calculated to be smaller than the DKH2

  6. Grid and basis adaptive polynomial chaos techniques for sensitivity and uncertainty analysis

    SciTech Connect (OSTI)

    Perk, Zoltn Gilli, Luca Lathouwers, Danny Kloosterman, Jan Leen

    2014-03-01

    The demand for accurate and computationally affordable sensitivity and uncertainty techniques is constantly on the rise and has become especially pressing in the nuclear field with the shift to Best Estimate Plus Uncertainty methodologies in the licensing of nuclear installations. Besides traditional, already well developed methods such as first order perturbation theory or Monte Carlo sampling Polynomial Chaos Expansion (PCE) has been given a growing emphasis in recent years due to its simple application and good performance. This paper presents new developments of the research done at TU Delft on such Polynomial Chaos (PC) techniques. Our work is focused on the Non-Intrusive Spectral Projection (NISP) approach and adaptive methods for building the PCE of responses of interest. Recent efforts resulted in a new adaptive sparse grid algorithm designed for estimating the PC coefficients. The algorithm is based on Gerstner's procedure for calculating multi-dimensional integrals but proves to be computationally significantly cheaper, while at the same it retains a similar accuracy as the original method. More importantly the issue of basis adaptivity has been investigated and two techniques have been implemented for constructing the sparse PCE of quantities of interest. Not using the traditional full PC basis set leads to further reduction in computational time since the high order grids necessary for accurately estimating the near zero expansion coefficients of polynomial basis vectors not needed in the PCE can be excluded from the calculation. Moreover the sparse PC representation of the response is easier to handle when used for sensitivity analysis or uncertainty propagation due to the smaller number of basis vectors. The developed grid and basis adaptive methods have been implemented in Matlab as the Fully Adaptive Non-Intrusive Spectral Projection (FANISP) algorithm and were tested on four analytical problems. These show consistent good performance both in

  7. 105-K Basin material design basis feed description for spent nuclear fuel project facilities

    SciTech Connect (OSTI)

    Praga, A.N.

    1998-01-08

    Revisions 0 and 0A of this document provided estimated chemical and radionuclide inventories of spent nuclear fuel and sludge currently stored within the Hanford Site`s 105-K Basins. This Revision (Rev. 1) incorporates the following changes into Revision 0A: (1) updates the tables to reflect: improved cross section data, a decision to use accountability data as the basis for total Pu, a corrected methodology for selection of the heat generation basis fee, and a revised decay date; (2) adds section 3.3.3.1 to expand the description of the approach used to calculate the inventory values and explain why that approach yields conservative results; (3) changes the pre-irradiation braze beryllium value.

  8. FY2001 Tank Characterization Technical Sampling Basis & Waste Information Requirements Document

    SciTech Connect (OSTI)

    ADAMS, M.R.

    2000-08-02

    The Fiscal Year 2001 Tank Characterization Technical Sampling Basis and Waste Information Requirements Document (TSB-WIRD) has the following purposes: (1) To identify and integrate sampling and analysis needs for fiscal year (FY) 2001 and beyond. (2) To describe the overall drivers that require characterization information and to document their source. (3) To describe the process for identifying, prioritizing, and weighting issues that require characterization information to resolve. (4) To define the method for determining sampling priorities and to present the sampling priorities on a tank-by-tank basis. (5) To define how the characterization program is going to satisfy the drivers, close issues, and report progress. (6)To describe deliverables and acceptance criteria for characterization deliverables.

  9. Hamiltonian Light-front Field Theory Within an AdS/QCD Basis

    SciTech Connect (OSTI)

    Vary, J.P.; Honkanen, H.; Li, Jun; Maris, P.; Brodsky, S.J.; Harindranath, A.; de Teramond, G.F.; Sternberg, P.; Ng, E.G.; Yang, C.; /LBL, Berkeley

    2009-12-16

    Non-perturbative Hamiltonian light-front quantum field theory presents opportunities and challenges that bridge particle physics and nuclear physics. Fundamental theories, such as Quantum Chromodynamics (QCD) and Quantum Electrodynamics (QED) offer the promise of great predictive power spanning phenomena on all scales from the microscopic to cosmic scales, but new tools that do not rely exclusively on perturbation theory are required to make connection from one scale to the next. We outline recent theoretical and computational progress to build these bridges and provide illustrative results for nuclear structure and quantum field theory. As our framework we choose light-front gauge and a basis function representation with two-dimensional harmonic oscillator basis for transverse modes that corresponds with eigensolutions of the soft-wall AdS/QCD model obtained from light-front holography.

  10. Comparison of CRBR design-basis events with those of foreign LMFBR plants

    SciTech Connect (OSTI)

    Agrawal, A.K.

    1983-04-01

    As part of the Construction Permit (CP) review of the Clinch River Breeder Reactor Plant (CRBR), the Brookhaven National Laboratory was asked to compare the Design Basis Accidents that are considered in CRBR Preliminary Safety Analysis Report with those of the foreign contemporary plants (PHENIX, SUPER-PHENIX, SNR-300, PFR, and MONJU). A brief introductory review of any special or unusual characteristics of these plants is given. This is followed by discussions of the design basis accidents and their acceptance criteria. In spite of some discrepancies due either to semantics or to licensing decisions, there appears to be a considerable degree of unanimity in the selection (definition) of DBAs in all of these plants.

  11. Kinetically balanced Gaussian basis-set approach to relativistic Compton profiles of atoms

    SciTech Connect (OSTI)

    Jaiswal, Prerit; Shukla, Alok

    2007-02-15

    Atomic Compton profiles (CPs) are a very important property which provide us information about the momentum distribution of atomic electrons. Therefore, for CPs of heavy atoms, relativistic effects are expected to be important, warranting a relativistic treatment of the problem. In this paper, we present an efficient approach aimed at ab initio calculations of atomic CPs within a Dirac-Hartree-Fock (DHF) formalism, employing kinetically balanced Gaussian basis functions. The approach is used to compute the CPs of noble gases ranging from He to Rn, and the results have been compared to the experimental and other theoretical data, wherever possible. The influence of the quality of the basis set on the calculated CPs has also been systematically investigated.

  12. Polychromatic sparse image reconstruction and mass attenuation spectrum estimation via B-spline basis function expansion

    SciTech Connect (OSTI)

    Gu, Renliang E-mail: ald@iastate.edu; Dogandžić, Aleksandar E-mail: ald@iastate.edu

    2015-03-31

    We develop a sparse image reconstruction method for polychromatic computed tomography (CT) measurements under the blind scenario where the material of the inspected object and the incident energy spectrum are unknown. To obtain a parsimonious measurement model parameterization, we first rewrite the measurement equation using our mass-attenuation parameterization, which has the Laplace integral form. The unknown mass-attenuation spectrum is expanded into basis functions using a B-spline basis of order one. We develop a block coordinate-descent algorithm for constrained minimization of a penalized negative log-likelihood function, where constraints and penalty terms ensure nonnegativity of the spline coefficients and sparsity of the density map image in the wavelet domain. This algorithm alternates between a Nesterov’s proximal-gradient step for estimating the density map image and an active-set step for estimating the incident spectrum parameters. Numerical simulations demonstrate the performance of the proposed scheme.

  13. Licensing topical report: application of probabilistic risk assessment in the selection of design basis accidents. [HTGR

    SciTech Connect (OSTI)

    Houghton, W.J.

    1980-06-01

    A probabilistic risk assessment (PRA) approach is proposed to be used to scrutinize selection of accident sequences. A technique is described in this Licensing Topical Report to identify candidates for Design Basis Accidents (DBAs) utilizing the risk assessment results. As a part of this technique, it is proposed that events with frequencies below a specified limit would not be candidates. The use of the methodology described is supplementary to the traditional, deterministic approach and may result, in some cases, in the selection of multiple failure sequences as DBAs; it may also provide a basis for not considering some traditionally postulated events as being DBAs. A process is then described for selecting a list of DBAs based on the candidates from PRA as supplementary to knowledge and judgments from past licensing practice. These DBAs would be the events considered in Chapter 15 of Safety Analysis Reports of high-temperature gas-cooled reactors (HTGRs).

  14. Review and Approval of Nuclear Facility Safety Basis Documents (Documented Safety Analyses and Technical Safety Requirements)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE-STD-1104-96 November 2005 CHANGE NOTICE NO. 3 Date December 2005 DOE STANDARD REVIEW AND APPROVAL OF NUCLEAR FACILITY SAFETY BASIS DOCUMENTS (DOCUMENTED SAFETY ANALYSES AND TECHNICAL SAFETY REQUIREMENTS) U.S. Department of Energy AREA SAFT Washington, DC 20585 DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. This document has been reproduced directly from the best available copy. Available to DOE and DOE contractors from ES&H Technical Information

  15. Technical Basis for Work Place Air Monitoring for the Plutonium Finishing Plan (PFP)

    SciTech Connect (OSTI)

    JONES, R.A.

    1999-10-06

    This document establishes the basis for the Plutonium Finishing Plant's (PFP) work place air monitoring program in accordance with the following requirements: Title 10, Code of Federal Regulations (CFR), Part 835 ''Occupational Radiation Protection''; Hanford Site Radiological Control Manual (HSRCM-1); HNF-PRO-33 1, Work Place Air Monitoring; WHC-SD-CP-SAR-021, Plutonium Finishing Plant Final Safety Analysis Report; and Applicable recognized national standards invoked by DOE Orders and Policies.

  16. Hydro-Kansas (HK) Research Project: Tests of a Physical Basis of

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Statistical Self-Similarity in Peak Flows in the Whitewater Basin, Kansas Hydro-Kansas (HK) Research Project: Tests of a Physical Basis of Statistical Self-Similarity in Peak Flows in the Whitewater Basin, Kansas Gupta, Vijay University of Colorado Furey, Peter Colorado Research Associates Mantila, Ricardo University of Colorado Krajewski, Witold University of Iowa Kruger, Anton The University of Iowa Clayton, Jordan US Geological Survey and University of Iowa Category: Atmospheric State and

  17. Prediction of storage life of hydraulic oils on the basis of accelerated climatic tests

    SciTech Connect (OSTI)

    Lovzin, E.V.; Polyakova, A.A.; Semanyuk, R.N.; Fal`kovskaya, O.I.; Shabalina, T.N.; Tyshchenko, V.A.; Kalinina, L.D.

    1994-09-01

    On the basis of changes in physicochemical characteristics of hydraulic oil (kinematic viscosity, solid point, refractive index, density) under the influence of conditions of accelerated climatic tests (ACTs), it is impossible to judge the changes of oil composition with any degree of reliability. Of the components of hydraulic oil, the most sensitive to the combined action of temperature, moisture, and various metals are the aromatic hydrocarbons, oxygen-containing compounds, and the antioxidant diphenylamine.

  18. Integrated Safety Management System as the Basis for Work Planning and Control for Research and Development

    Broader source: Energy.gov [DOE]

    Slide Presentation by Rich Davies, Kami Lowry, Mike Schlender, Pacific Northwest National Laboratory (PNNL) and Ted Pietrok, Pacific Northwest Site Office (PNSO). Integrated Safety Management System as the Basis for Work Planning and Control for Research and Development. Work Planning and Control (WP&C) is essential to assuring the safety of workers and the public regardless of the scope of work Research and Development (R&D) activities are no exception.

  19. A probabilistic risk assessment of the LLNL Plutonium facility`s evaluation basis fire operational accident

    SciTech Connect (OSTI)

    Brumburgh, G.

    1994-08-31

    The Lawrence Livermore National Laboratory (LLNL) Plutonium Facility conducts numerous involving plutonium to include device fabrication, development of fabrication techniques, metallurgy research, and laser isotope separation. A Safety Analysis Report (SAR) for the building 332 Plutonium Facility was completed rational safety and acceptable risk to employees, the public, government property, and the environment. This paper outlines the PRA analysis of the Evaluation Basis Fire (EDF) operational accident. The EBF postulates the worst-case programmatic impact event for the Plutonium Facility.

  20. Tank waste remediation system retrieval and disposal mission authorization basis amendment task plan

    SciTech Connect (OSTI)

    Goetz, T.G.

    1998-01-08

    This task plan is a documented agreement between Nuclear Safety and Licensing and the Process Development group within the Waste Feed Delivery organization. The purpose of this task plan is to identify the scope of work, tasks and deliverables, responsibilities, manpower, and schedules associated with an authorization basis amendment as a result of the Waste Feed Waste Delivery Program, Project W-211, and Project W-TBD.

  1. Technical Basis for U. S. Department of Energy Nuclear Safety Policy, DOE Policy 420.1

    Office of Energy Efficiency and Renewable Energy (EERE)

    This document provides the technical basis for the Department of Energy (DOE) Policy (P) 420.1, Nuclear Safety Policy, dated 2-8-2011. It includes an analysis of the revised Policy to determine whether it provides the necessary and sufficient high-level expectations that will lead DOE to establish and implement appropriate requirements to assure protection of the public, workers, and the environment from the hazards of DOE’s operation of nuclear facilities.

  2. Safety basis for the 241-AN-107 mixer pump installation and caustic addition

    SciTech Connect (OSTI)

    Van Vleet, R.J.

    1994-10-05

    This safety Basis was prepared to determine whether or not the proposed activities of installing a 76 HP jet mixer pump and the addition of approximately 50,000 gallons of 19 M (50:50 wt %) aqueous caustic are within the safety envelope as described by Tank Farms (chapter six of WHC-SD-WM-ISB-001, Rev. 0). The safety basis covers the components, structures and systems for the caustic addition and mixer pump installation. These include: installation of the mixer pump and monitoring equipment; operation of the mixer pump, process monitoring equipment and caustic addition; the pump stand, caustic addition skid, the electrical skid, the video camera system and the two densitometers. Also covered is the removal and decontamination of the mixer pump and process monitoring system. Authority for this safety basis is WHC-IP-0842 (Waste Tank Administration). Section 15.9, Rev. 2 (Unreviewed Safety Questions) of WHC-IP-0842 requires that an evaluation be performed for all physical modifications.

  3. Demonstrating Structural Adequacy of Nuclear Power Plant Containment Structures for Beyond Design-Basis Pressure Loadings

    SciTech Connect (OSTI)

    Braverman, J.I.; Morante, R.

    2010-07-18

    ABSTRACT Demonstrating the structural integrity of U.S. nuclear power plant (NPP) containment structures, for beyond design-basis internal pressure loadings, is necessary to satisfy Nuclear Regulatory Commission (NRC) requirements and performance goals. This paper discusses methods for demonstrating the structural adequacy of the containment for beyond design-basis pressure loadings. Three distinct evaluations are addressed: (1) estimating the ultimate pressure capacity of the containment structure (10 CFR 50 and US NRC Standard Review Plan, Section 3.8) ; (2) demonstrating the structural adequacy of the containment subjected to pressure loadings associated with combustible gas generation (10 CFR 52 and 10 CFR 50); and (3) demonstrating the containment structural integrity for severe accidents (10 CFR 52 as well as SECY 90-016, SECY 93-087, and related NRC staff requirements memoranda (SRMs)). The paper describes the technical basis for specific aspects of the methods presented. It also presents examples of past issues identified in licensing activities related to these evaluations.

  4. Central Facilities Area Facilities Radioactive Waste Management Basis and DOE Manual 435.1-1 Compliance Tables

    SciTech Connect (OSTI)

    Lisa Harvego; Brion Bennett

    2011-11-01

    Department of Energy Order 435.1, 'Radioactive Waste Management,' along with its associated manual and guidance, requires development and maintenance of a radioactive waste management basis for each radioactive waste management facility, operation, and activity. This document presents a radioactive waste management basis for Idaho National Laboratory's Central Facilities Area facilities that manage radioactive waste. The radioactive waste management basis for a facility comprises existing laboratory-wide and facilityspecific documents. Department of Energy Manual 435.1-1, 'Radioactive Waste Management Manual,' facility compliance tables also are presented for the facilities. The tables serve as a tool for developing the radioactive waste management basis.

  5. Research and Education Campus Facilities Radioactive Waste Management Basis and DOE Manual 435.1-1 Compliance Tables

    SciTech Connect (OSTI)

    L. Harvego; Brion Bennett

    2011-11-01

    U.S. Department of Energy Order 435.1, 'Radioactive Waste Management,' along with its associated manual and guidance, requires development and maintenance of a radioactive waste management basis for each radioactive waste management facility, operation, and activity. This document presents a radioactive waste management basis for Idaho National Laboratory Research and Education Campus facilities that manage radioactive waste. The radioactive waste management basis for a facility comprises existing laboratory-wide and facility-specific documents. Department of Energy Manual 435.1-1, 'Radioactive Waste Management Manual,' facility compliance tables also are presented for the facilities. The tables serve as a tool to develop the radioactive waste management basis.

  6. Materials and Security Consolidation Complex Facilities Radioactive Waste Management Basis and DOE Manual 435.1-1 Compliance Tables

    SciTech Connect (OSTI)

    Not Listed

    2011-09-01

    Department of Energy Order 435.1, 'Radioactive Waste Management,' along with its associated manual and guidance, requires development and maintenance of a radioactive waste management basis for each radioactive waste management facility, operation, and activity. This document presents a radioactive waste management basis for Idaho National Laboratory's Materials and Security Consolidation Center facilities that manage radioactive waste. The radioactive waste management basis for a facility comprises existing laboratory-wide and facility-specific documents. Department of Energy Manual 435.1-1, 'Radioactive Waste Management Manual,' facility compliance tables also are presented for the facilities. The tables serve as a tool for developing the radioactive waste management basis.

  7. Materials and Fuels Complex Facilities Radioactive Waste Management Basis and DOE Manual 435.1-1 Compliance Tables

    SciTech Connect (OSTI)

    Lisa Harvego; Brion Bennett

    2011-09-01

    Department of Energy Order 435.1, 'Radioactive Waste Management,' along with its associated manual and guidance, requires development and maintenance of a radioactive waste management basis for each radioactive waste management facility, operation, and activity. This document presents a radioactive waste management basis for Idaho National Laboratory's Materials and Fuels Complex facilities that manage radioactive waste. The radioactive waste management basis for a facility comprises existing laboratory-wide and facility-specific documents. Department of Energy Manual 435.1-1, 'Radioactive Waste Management Manual,' facility compliance tables also are presented for the facilities. The tables serve as a tool for developing the radioactive waste management basis.

  8. Just in Time DSA-The Hanford Nuclear Safety Basis Strategy

    SciTech Connect (OSTI)

    Olinger, S. J.; Buhl, A. R.

    2002-02-26

    The U.S. Department of Energy, Richland Operations Office (RL) is responsible for 30 hazard category 2 and 3 nuclear facilities that are operated by its prime contractors, Fluor Hanford Incorporated (FHI), Bechtel Hanford, Incorporated (BHI) and Pacific Northwest National Laboratory (PNNL). The publication of Title 10, Code of Federal Regulations, Part 830, Subpart B, Safety Basis Requirements (the Rule) in January 2001 imposed the requirement that the Documented Safety Analyses (DSA) for these facilities be reviewed against the requirements of the Rule. Those DSA that do not meet the requirements must either be upgraded to satisfy the Rule, or an exemption must be obtained. RL and its prime contractors have developed a Nuclear Safety Strategy that provides a comprehensive approach for supporting RL's efforts to meet its long term objectives for hazard category 2 and 3 facilities while also meeting the requirements of the Rule. This approach will result in a reduction of the total number of safety basis documents that must be developed and maintained to support the remaining mission and closure of the Hanford Site and ensure that the documentation that must be developed will support: compliance with the Rule; a ''Just-In-Time'' approach to development of Rule-compliant safety bases supported by temporary exemptions; and consolidation of safety basis documents that support multiple facilities with a common mission (e.g. decontamination, decommissioning and demolition [DD&D], waste management, surveillance and maintenance). This strategy provides a clear path to transition the safety bases for the various Hanford facilities from support of operation and stabilization missions through DD&D to accelerate closure. This ''Just-In-Time'' Strategy can also be tailored for other DOE Sites, creating the potential for large cost savings and schedule reductions throughout the DOE complex.

  9. Technical basis for classification of low-activity waste fraction from Hanford site tanks

    SciTech Connect (OSTI)

    Petersen, C.A.

    1996-09-20

    The overall objective of this report is to provide a technical basis to support a U.S. Nuclear Regulatory Commission determination to classify the low-activity waste from the Hanford Site single-shell and double-shell tanks as `incidental` wastes after removal of additional radionuclides and immobilization.The proposed processing method, in addition to the previous radionuclide removal efforts, will remove the largest practical amount of total site radioactivity, attributable to high-level waste, for disposal is a deep geologic repository. The remainder of the waste would be considered `incidental` waste and could be disposed onsite.

  10. Technical basis for classification of low-activity waste fraction from Hanford site tanks

    SciTech Connect (OSTI)

    Petersen, C.A., Westinghouse Hanford

    1996-07-17

    The overall objective of this report is to provide a technical basis to support a U.S. Nuclear Regulatory Commission determination to classify the low-activity waste from the Hanford Site single-shell and double-shell tanks as `incidental` wastes after removal of additional radionuclides and immobilization.The proposed processing method, in addition to the previous radionuclide removal efforts, will remove the largest practical amount of total site radioactivity, attributable to high-level wastes, for disposal in a deep geologic repository. The remainder of the waste would be considered `incidental` waste and could be disposed onsite.

  11. Technical basis for cases N-629 and N-631 as an alternative for RTNDT reference temperature

    SciTech Connect (OSTI)

    Merkle, John Graham; Server, W. L.

    2007-01-01

    ASME Code Cases N-629/N-631, published in 1999, provided an important new approach to allow material specific, measured fracture toughness curves for ferritic steels in the code applications. This has enabled some of the nuclear power plants whose reactor pressure vessel materials reached a certain threshold level based on overly conservative rules to use an alternative RTNDT to justify continued operation of their plants. These code cases have been approved by the US Nuclear Regulatory Commission and these have been proposed to be codified in Appendix A and Appendix G of the ASME Boiler and Pressure Vessel Code. This paper summarizes the basis of this approach for the record.

  12. Convergent conductivity corrections to the Casimir force via exponential basis functions

    SciTech Connect (OSTI)

    Cui, Song; Soh, Yeng Chai

    2010-12-15

    A closed-form finite conductivity correction factor for the ideal Casimir force is proposed, based on exponential basis functions. Our method can facilitate experimental verifications of theories in the study of the Casimir force. A theoretical analysis is given to explain why our method is accurate at both large and small separation gaps. Numerical computations have been performed to confirm that our method is accurate in various experimental configurations. Our approach is widely applicable to various Casimir force interactions between metals and dielectrics. Our study can be extended to the study of the repulsive Casimir force as well.

  13. Structural basis of glycan specificity in neonate-specific bovine-human reassortant rotavirus

    SciTech Connect (OSTI)

    Hu, Liya; Ramani, Sasirekha; Czako, Rita; Sankaran, Banumathi; Yu, Ying; Smith, David F.; Cummings, Richard D.; Estes, Mary K.; Venkataram Prasad, B. V.

    2015-09-30

    We report that strain-dependent variation of glycan recognition during initial cell attachment of viruses is a critical determinant of host specificity, tissue-tropism and zoonosis. Rotaviruses (RVs), which cause life-threatening gastroenteritis in infants and children, display significant genotype-dependent variations in glycan recognition resulting from sequence alterations in the VP8* domain of the spike protein VP4. The structural basis of this genotype-dependent glycan specificity, particularly in human RVs, remains poorly understood. Here, from crystallographic studies, we show how genotypic variations configure a novel binding site in the VP8* of a neonate-specific bovine-human reassortant to uniquely recognize either type I or type II precursor glycans, and to restrict type II glycan binding in the bovine counterpart. In conclusion, such a distinct glycan-binding site that allows differential recognition of the precursor glycans, which are developmentally regulated in the neonate gut and abundant in bovine and human milk provides a basis for age-restricted tropism and zoonotic transmission of G10P[11] rotaviruses.

  14. Engineering Basis Document Review Supporting the Double Shell Tank (DST) System Specification Development

    SciTech Connect (OSTI)

    LEONARD, M.W.

    2000-03-14

    The Double-Shell Tank (DST) System is required to transition from its current storage mission to a storage and retrieval mission supporting the River Protection Project Phase 1 privatization, defined in HNF-SD-WM-MAR-008, Tank Waste Remediation System Mission Analysis Report. Requirements for the DST subsystems are being developed using the top-down systems engineering process outlined in HNF-SD-WM-SEMP-002, Tank Waste Remediation System Systems Engineering Management Plan. This top-down process considers existing designs to the extent that these designs impose unavoidable constraints on the Phase 1 mission. Existing engineering-basis documents were screened, and the unavoidable constraints were identified. The constraints identified herein will be added to the DST System specification (HNF-SD-WM-TRD-007, System Specification for the Double-Shell Tank System). While the letter revisions of the DST System specification were constructed with a less rigorous review of the existing engineering-basis documents, the Revision 0 release of the specification must incorporate the results of the review documented herein. The purpose of this document is to describe the screening process and criteria used to determine which constraints are unavoidable and to document the screening results.

  15. AP1000 Design Basis Event Simulation at the APEX-1000 Test Facility

    SciTech Connect (OSTI)

    Wright, Richard F.; Groome, John

    2004-07-01

    The AP1000 is a 1000 MWe advanced nuclear power plant that uses passive safety features to enhance plant safety and to provide significant and measurable improvements in plant simplification, reliability, investment protection and plant costs. The AP1000 relies heavily on the 600 MWe AP600 which received design certification in 1999. A critical part of the AP600 design certification process involved the testing of the passive safety systems. A one-fourth height, one-fourth pressure test facility, APEX-600, was constructed at the Oregon State University to study design basis events, and to provide a body of data to be used to validate the computer models used to analyze the AP600. This facility was extensively modified to reflect the design changes for AP1000 including higher power in the electrically heated rods representing the reactor core, and changes in the size of the pressurizer, core makeup tanks and automatic depressurization system. Several design basis events are being simulated at APEX-1000 including a double-ended direct vessel injection (DEDVI) line break and a 2-inch cold leg break. These tests show that the core remains covered with ample margin until gravity injection is established regardless of the initiating event. The tests also show that liquid entrainment from the upper plenum which is proportional to the reactor power does not impact the ability of the passive core cooling system to keep the core covered. (authors)

  16. Computing single step operators of logic programming in radial basis function neural networks

    SciTech Connect (OSTI)

    Hamadneh, Nawaf; Sathasivam, Saratha; Choon, Ong Hong

    2014-07-10

    Logic programming is the process that leads from an original formulation of a computing problem to executable programs. A normal logic program consists of a finite set of clauses. A valuation I of logic programming is a mapping from ground atoms to false or true. The single step operator of any logic programming is defined as a function (T{sub p}:I→I). Logic programming is well-suited to building the artificial intelligence systems. In this study, we established a new technique to compute the single step operators of logic programming in the radial basis function neural networks. To do that, we proposed a new technique to generate the training data sets of single step operators. The training data sets are used to build the neural networks. We used the recurrent radial basis function neural networks to get to the steady state (the fixed point of the operators). To improve the performance of the neural networks, we used the particle swarm optimization algorithm to train the networks.

  17. Development of Probabilistic Design Basis Earthquake (DBE) Parameters for Moderate and High Hazard Facilities at INEEL

    SciTech Connect (OSTI)

    S. M. Payne; V. W. Gorman; S. A. Jensen; M. E. Nitzel; M. J. Russell; R. P. Smith

    2000-03-01

    Design Basis Earthquake (DBE) horizontal and vertical response spectra are developed for moderate and high hazard facilities or Performance Categories (PC) 3 and 4, respectively, at the Idaho National Engineering and Environmental Laboratory (INEEL). The probabilistic DBE response spectra will replace the deterministic DBE response spectra currently in the U.S. Department of Energy Idaho Operations Office (DOE-ID) Architectural Engineering Standards that govern seismic design criteria for several facility areas at the INEEL. Probabilistic DBE response spectra are recommended to DOE Naval Reactors for use at the Naval Reactor Facility at INEEL. The site-specific Uniform Hazard Spectra (UHS) developed by URS Greiner Woodward Clyde Federal Services are used as the basis for developing the DBE response spectra. In 1999, the UHS for all INEEL facility areas were recomputed using more appropriate attenuation relationships for the Basin and Range province. The revised UHS have lower ground motions than those produced in the 1996 INEEL site-wide probabilistic ground motion study. The DBE response spectra were developed by incorporating smoothed broadened regions of the peak accelerations, velocities, and displacements defined by the site-specific UHS. Portions of the DBE response spectra were adjusted to ensure conservatism for the structural design process.

  18. Technical basis and proposal for deriving sediment quality criteria for metals

    SciTech Connect (OSTI)

    Ankley, G.T.; Toro, D.M. di |; Hansen, D.J.; Berry, W.J.

    1996-12-01

    In developing sediment quality criteria (SQC) for metals, it is essential that bioavailability be a prime consideration. Different studies have shown that while dry weight metal concentrations in sediments are not predictive of bioavailability, metal concentrations in interstitial (pore) water are correlated with observed biological effects. A key partitioning phase controlling cationic metal activity and toxicity in the sediment-interstitial water system is acid-volatile sulfide (AVS). Acid-volatile sulfide binds, on a mole-to-mole basis, a number of cationic metals of environmental concern (cadmium, copper, nickel, lead, zinc) forming insoluble sulfide complexes with minimal biological availability. Short-term (10-d) laboratory studies with a variety of marine and freshwater benthic organisms have demonstrated that when AVS concentrations in spiked or field-collected sediments exceed those of metals simultaneously extracted with the AVS, interstitial water metal concentrations remain below those predicted to cause effects, and toxicity does not occur. Similar observations have been made in life-cycle laboratory toxicity tests with amphipods and chironomids in marine and freshwater sediments spiked with cadmium and zinc, respectively. In addition, field colonization experiments, varying in length from several months to more than 1 year, with cadmium- or zinc-spiked freshwater and marine sediments, have demonstrated a lack of biological effects when there is sufficient AVS to limit interstitial water metal concentrations. These studies on metal bioavailability and toxicity in sediments serve as the basis for proposed SQC for the metals cadmium, copper, nickel, lead, and zinc.

  19. Preliminary performance assessment for the Waste Isolation Pilot Plant, December 1992. Volume 2, Technical basis

    SciTech Connect (OSTI)

    Not Available

    1992-12-01

    Before disposing of transuranic radioactive waste in the Waste Isolation Pilot Plant (WIPP), the United States Department of Energy (DOE) must evaluate compliance with applicable long-term regulations of the United States Environmental Protection Agency (EPA). Sandia National Laboratories is conducting iterative performance assessments (PAs) of the WIPP for the DOE to provide interim guidance while preparing for a final compliance evaluation. This volume, Volume 2, contains the technical basis for the 1992 PA. Specifically, it describes the conceptual basis for consequence modeling and the PA methodology, including the selection of scenarios for analysis, the determination of scenario probabilities, and the estimation of scenario consequences using a Monte Carlo technique and a linked system of computational models. Additional information about the 1992 PA is provided in other volumes. Volume I contains an overview of WIPP PA and results of a preliminary comparison with the long-term requirements of the EPA`s Environmental Protection Standards for Management and Disposal of Spent Nuclear Fuel, High-Level and Transuranic Radioactive Wastes (40 CFR 191, Subpart B). Volume 3 contains the reference data base and values for input parameters used in consequence and probability modeling. Volume 4 contains uncertainty and sensitivity analyses related to the preliminary comparison with 40 CFR 191B. Volume 5 contains uncertainty and sensitivity analyses of gas and brine migration for undisturbed performance. Finally, guidance derived from the entire 1992 PA is presented in Volume 6.

  20. Authorization Basis Safety Classification of Transfer Bay Bridge Crane at the 105-K Basins

    SciTech Connect (OSTI)

    CHAFFEE, G.A.

    2000-04-06

    This supporting document provides the bases for the safety classification for the K Basin transfer bay bridge crane and the bases for the Structures, Systems, and Components (SSC) safety classification. A table is presented that delineates the safety significant components. This safety classification is based on a review of the Authorization Basis (AB). This Authorization Basis review was performed regarding AB and design baseline issues. The primary issues are: (1) What is the AB for the safety classification of the transfer bay bridge crane? (2) What does the SSC safety classification ''Safety Significant'' or ''Safety Significant for Design Only'' mean for design requirements and quality requirements for procurement, installation and maintenance (including replacement of parts) activities for the crane during its expected life time? The AB information on the crane was identified based on review of Department of Energy--Richland Office (RL) and Spent Nuclear Fuel (SNF) Project correspondence, K Basin Safety Analysis Report (SAR) and RL Safety Evaluation Reports (SERs) of SNF Project SAR submittals. The relevant correspondence, actions and activities taken and substantive directions or conclusions of these documents are provided in Appendix A.

  1. Current plans to characterize the design basis ground motion at the Yucca Mountain, Nevada Site

    SciTech Connect (OSTI)

    Simecka, W.B.; Grant, T.A.; Voegele, M.D.; Cline, K.M.

    1992-12-31

    A site at Yucca Mountain Nevada is currently being studied to assess its suitability as a potential host site for the nation`s first commercial high level waste repository. The DOE has proposed a new methodology for determining design-basis ground motions that uses both deterministic and probabilistic methods. The role of the deterministic approach is primary. It provides the level of detail needed by design engineers in the characterization of ground motions. The probabilistic approach provides a logical structured procedure for integrating the range of possible earthquakes that contribute to the ground motion hazard at the site. In addition, probabilistic methods will be used as needed to provide input for the assessment of long-term repository performance. This paper discusses the local tectonic environment, potential seismic sources and their associated displacements and ground motions. It also discusses the approach to assessing the design basis earthquake for the surface and underground facilities, as well as selected examples of the use of this type of information in design activities.

  2. Structural basis of glycan specificity in neonate-specific bovine-human reassortant rotavirus

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Hu, Liya; Ramani, Sasirekha; Czako, Rita; Sankaran, Banumathi; Yu, Ying; Smith, David F.; Cummings, Richard D.; Estes, Mary K.; Venkataram Prasad, B. V.

    2015-09-30

    We report that strain-dependent variation of glycan recognition during initial cell attachment of viruses is a critical determinant of host specificity, tissue-tropism and zoonosis. Rotaviruses (RVs), which cause life-threatening gastroenteritis in infants and children, display significant genotype-dependent variations in glycan recognition resulting from sequence alterations in the VP8* domain of the spike protein VP4. The structural basis of this genotype-dependent glycan specificity, particularly in human RVs, remains poorly understood. Here, from crystallographic studies, we show how genotypic variations configure a novel binding site in the VP8* of a neonate-specific bovine-human reassortant to uniquely recognize either type I or type IImore » precursor glycans, and to restrict type II glycan binding in the bovine counterpart. In conclusion, such a distinct glycan-binding site that allows differential recognition of the precursor glycans, which are developmentally regulated in the neonate gut and abundant in bovine and human milk provides a basis for age-restricted tropism and zoonotic transmission of G10P[11] rotaviruses.« less

  3. Strategic Basis for License Application Planning for a Potential Yucca Mountain Repository

    SciTech Connect (OSTI)

    Newberry, C. M.; Brocoum, S. J.; Gamble, R. P.; Murray, R. C.; Cline, M.

    2002-02-26

    If Yucca Mountain, Nevada is designated as the site for development of a geologic repository for disposal of spent nuclear fuel and high-level radioactive waste, the Department of Energy (DOE) must obtain Nuclear Regulatory Commission (NRC) approval first for repository construction, then for an operating license, and, eventually, for repository closure and decommissioning. The licensing criteria defined in Code of Federal Regulations, Title 10, Part 63 (10 CFR Part 63) establish the basis for these NRC decisions. Submittal of a license application (LA) to the NRC for authorization to construct a repository at the Yucca Mountain site is, at this point, only a potential future action by the DOE. The policy process defined in the Nuclear Waste Policy Act (NWPA), as amended, for recommendation and designation of Yucca Mountain as a repository site makes it difficult to predict whether or when the site might be designated. The DOE may only submit a LA to the NRC if the site designation takes effect. In spite of this uncertainty, the DOE must take prudent and appropriate action now, and over the next several years, to prepare for development and timely submittal of a LA. This is particularly true given the need for the DOE to develop, load, and certify the operation of its electronic information system to provide access to its relevant records as part of the licensing support network (LSN) in compliance with NRC requirements six months prior to LA submittal. The DOE must also develop a LA, which is a substantially different document from those developed to support a Site Recommendation (SR) decision. The LA must satisfy NRC licensing criteria and content requirements, and address the acceptance criteria defined by the NRC in its forthcoming Yucca Mountain Review Plan (YMRP). The content of the LA must be adequate to facilitate NRC acceptance and docketing for review, and the LA and its supporting documents must provide the documented basis for the NR C findings required

  4. U.S. Department of Energy, Oak Ridge Operations Office Nuclear Facility Safety Basis Fundamentals Self-Study Guide [Fulfills ORO Safety Basis Competency 1, 2 (Part 1), or 7 (Part 1)

    Office of Energy Efficiency and Renewable Energy (EERE)

    "This self-study guide provides an overview of safety basis terminology, requirements, and activities that are applicable to DOE and Oak Ridge Operations Office (ORO) nuclear facilities on the Oak...

  5. Micrometer-scale fabrication of complex three dimensional lattice + basis structures in silicon

    SciTech Connect (OSTI)

    Burckel, D. Bruce; Resnick, Paul J.; Finnegan, Patrick S.; Sinclair, Michael B.; Davids, Paul S.

    2015-01-01

    A complementary metal oxide semiconductor (CMOS) compatible version of membrane projection lithography (MPL) for fabrication of micrometer-scale three-dimensional structures is presented. The approach uses all inorganic materials and standard CMOS processing equipment. In a single layer, MPL is capable of creating all 5 2D-Bravais lattices. Furthermore, standard semiconductor processing steps can be used in a layer-by-layer approach to create fully three dimensional structures with any of the 14 3D-Bravais lattices. The unit cell basis is determined by the projection of the membrane pattern, with many degrees of freedom for defining functional inclusions. Here we demonstrate several unique structural motifs, and characterize 2D arrays of unit cells with split ring resonators in a silicon matrix. The structures exhibit strong polarization dependent resonances and, for properly oriented split ring resonators (SRRs), coupling to the magnetic field of a normally incident transverse electromagnetic wave, a response unique to 3D inclusions.

  6. Structural basis of GDP release and gating in G protein coupled Fe[superscript 2+] transport

    SciTech Connect (OSTI)

    Guilfoyle, Amy; Maher, Megan J.; Rapp, Mikaela; Clarke, Ronald; Harrop, Stephen; Jormakka, Mika

    2009-09-29

    G proteins are key molecular switches in the regulation of membrane protein function and signal transduction. The prokaryotic membrane protein FeoB is involved in G protein coupled Fe{sup 2+} transport, and is unique in that the G protein is directly tethered to the membrane domain. Here, we report the structure of the soluble domain of FeoB, including the G protein domain, and its assembly into an unexpected trimer. Comparisons between nucleotide free and liganded structures reveal the closed and open state of a central cytoplasmic pore, respectively. In addition, these data provide the first observation of a conformational switch in the nucleotide-binding G5 motif, defining the structural basis for GDP release. From these results, structural parallels are drawn to eukaryotic G protein coupled membrane processes.

  7. Composition and Technical Basis for K Basin Settler Sludge Simulant for Inspection, Retrieval, and Pump Testing

    SciTech Connect (OSTI)

    Schmidt, Andrew J.; Zacher, Alan H.

    2007-06-25

    This report provides the formulation and technical basis for a K Basin Settler Tank Sludge simulant that will be used by the K Basin Closure Project (KBC) to test and develop equipment/approaches for Settler Tank sludge level measurement and retrieval in a mock-up test system of the actual Settler Tanks. The sludge simulant may also be used to demonstrate that the TOYO high pressure positive displacement pump design (reversing valves and hollow balls) is suitable for transfer of Settler Tank sludge from the K West (KW) Basin to the Cold Vacuum Drying Facility (CVDF) (~500 ft). As requested the by the K Basins Sludge Treatment Project (STP) the simulant is comprised of non-radioactive (and non-uranium) constituents.

  8. Scientific basis for risk assessment and management of uranium mill tailings

    SciTech Connect (OSTI)

    Not Available

    1986-01-01

    A National Research Council study panel, convened by the Board on Radioactive Waste Management, has examined the scientific basis for risk assessment and management of uranium mill tailings and issued this final report containing a number of recommendations. Chapter 1 provides a brief introduction to the problem. Chapter 2 examines the processes of uranium extraction and the mechanisms by which radionuclides and toxic chemicals contained in the ore can enter the environment. Chapter 3 is devoted to a review of the evidence on health risks associated with radon and its decay products. Chapter 4 provides a consideration of conventional and possible new technical alternatives for tailings management. Chapter 5 explores a number of issues of comparative risk, provides a brief history of uranium mill tailings regulation, and concludes with a discussion of choices that must be made in mill tailing risk management. 211 refs., 30 figs., 27 tabs.

  9. Breckinridge Project, initial effort. Report XI, Volume V. Critical review of the design basis. [Critical review

    SciTech Connect (OSTI)

    1982-01-01

    Report XI, Technical Audit, is a compendium of research material used during the Initial Effort in making engineering comparisons and decisions. Volumes 4 and 5 of Report XI present those studies which provide a Critical Review of the Design Basis. The Critical Review Report, prepared by Intercontinental Econergy Associates, Inc., summarizes findings from an extensive review of the data base for the H-Coal process design. Volume 4 presents this review and assessment, and includes supporting material; specifically, Design Data Tabulation (Appendix A), Process Flow Sheets (Appendix B), and References (Appendix C). Volume 5 is a continuation of the references of Appendix C. Studies of a proprietary nature are noted and referenced, but are not included in these volumes. They are included in the Limited Access versions of these reports and may be reviewed by properly cleared personnel in the offices of Ashland Synthetic Fuels, Inc.

  10. Criteria for calculating the efficiency of HEPA filters during and after design basis accidents

    SciTech Connect (OSTI)

    Bergman, W.; First, M.W.; Anderson, W.L.; Gilbert, H.; Jacox, J.W.

    1994-12-01

    We have reviewed the literature on the performance of high efficiency particulate air (HEPA) filters under normal and abnormal conditions to establish criteria for calculating the efficiency of HEPA filters in a DOE nonreactor nuclear facility during and after a Design Basis Accident (DBA). The literature review included the performance of new filters and parameters that may cause deterioration in the filter performance such as filter age, radiation, corrosive chemicals, seismic and rough handling, high temperature, moisture, particle clogging, high air flow and pressure pulses. The deterioration of the filter efficiency depends on the exposure parameters; in severe exposure conditions the filter will be structurally damaged and have a residual efficiency of 0%. Despite the many studies on HEPA filter performance under adverse conditions, there are large gaps and limitations in the data that introduce significant error in the estimates of HEPA filter efficiencies under DBA conditions. Because of this limitation, conservative values of filter efficiency were chosen when there was insufficient data.

  11. Maximal design basis accident of fusion neutron source DEMO-TIN

    SciTech Connect (OSTI)

    Kolbasov, B. N.

    2015-12-15

    When analyzing the safety of nuclear (including fusion) facilities, the maximal design basis accident at which the largest release of activity is expected must certainly be considered. Such an accident is usually the failure of cooling systems of the most thermally stressed components of a reactor (for a fusion facility, it is the divertor or the first wall). The analysis of safety of the ITER reactor and fusion power facilities (including hybrid fission–fusion facilities) shows that the initial event of such a design basis accident is a large-scale break of a pipe in the cooling system of divertor or the first wall outside the vacuum vessel of the facility. The greatest concern is caused by the possibility of hydrogen formation and the inrush of air into the vacuum chamber (VC) with the formation of a detonating mixture and a subsequent detonation explosion. To prevent such an explosion, the emergency forced termination of the fusion reaction, the mounting of shutoff valves in the cooling systems of the divertor and the first wall or blanket for reducing to a minimum the amount of water and air rushing into the VC, the injection of nitrogen or inert gas into the VC for decreasing the hydrogen and oxygen concentration, and other measures are recommended. Owing to a continuous feed-out of the molten-salt fuel mixture from the DEMO-TIN blanket with the removal period of 10 days, the radioactivity release at the accident will mainly be determined by tritium (up to 360 PBq). The activity of fission products in the facility will be up to 50 PBq.

  12. Advanced Test Reactor Complex Facilities Radioactive Waste Management Basis and DOE Manual 435.1-1 Compliance Tables

    SciTech Connect (OSTI)

    Lisa Harvego; Brion Bennett

    2011-11-01

    U.S. Department of Energy Order 435.1, 'Radioactive Waste Management,' along with its associated manual and guidance, requires development and maintenance of a radioactive waste management basis for each radioactive waste management facility, operation, and activity. This document presents a radioactive waste management basis for Idaho National Laboratory's Advanced Test Reactor Complex facilities that manage radioactive waste. The radioactive waste management basis for a facility comprises existing laboratory-wide and facility-specific documents. U.S. Department of Energy Manual 435.1-1, 'Radioactive Waste Management Manual,' facility compliance tables also are presented for the facilities. The tables serve as a tool to develop the radioactive waste management basis.

  13. AUDIT REPORT Follow-up on Nuclear Safety: Safety Basis and Quality Assurance at the Los Alamos National

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Nuclear Safety: Safety Basis and Quality Assurance at the Los Alamos National Laboratory DOE/IG-0941 July 2015 U.S. Department of Energy Office of Inspector General Office of Audits and Inspections Department of Energy Washington, DC 20585 July 16, 2015 MEMORANDUM FOR THE SECRETARY FROM: Gregory H. Friedman Inspector General SUBJECT: INFORMATION: Audit Report: "Follow-up on Nuclear Safety: Safety Basis and Quality Assurance at the Los Alamos National Laboratory" BACKGROUND A primary

  14. Technical Basis for Certification of Seismic Design Criteria for the Waste Treatment Plant, Hanford, Washington

    SciTech Connect (OSTI)

    Brouns, T.M.; Rohay, A.C. [Pacific Northwest National Laboratory, Richland, WA (United States); Youngs, R.R. [Geomatrix Consultants, Inc., Oakland, CA (United States); Costantino, C.J. [C.J. Costantino and Associates, Valley, NY (United States); Miller, L.F. [U.S. Department of Energy, Office of River Protection, Richland, WA (United States)

    2008-07-01

    In August 2007, Secretary of Energy Samuel W. Bodman approved the final seismic and ground motion criteria for the Waste Treatment and Immobilization Plant (WTP) at the Department of Energy's (DOE) Hanford Site. Construction of the WTP began in 2002 based on seismic design criteria established in 1999 and a probabilistic seismic hazard analysis completed in 1996. The design criteria were reevaluated in 2005 to address questions from the Defense Nuclear Facilities Safety Board (DNFSB), resulting in an increase by up to 40% in the seismic design basis. DOE announced in 2006 the suspension of construction on the pretreatment and high-level waste vitrification facilities within the WTP to validate the design with more stringent seismic criteria. In 2007, the U.S. Congress mandated that the Secretary of Energy certify the final seismic and ground motion criteria prior to expenditure of funds on construction of these two facilities. With the Secretary's approval of the final seismic criteria in the summer of 2007, DOE authorized restart of construction of the pretreatment and high-level waste vitrification facilities. The technical basis for the certification of seismic design criteria resulted from a two-year Seismic Boreholes Project that planned, collected, and analyzed geological data from four new boreholes drilled to depths of approximately 1400 feet below ground surface on the WTP site. A key uncertainty identified in the 2005 analyses was the velocity contrasts between the basalt flows and sedimentary interbeds below the WTP. The absence of directly-measured seismic shear wave velocities in the sedimentary interbeds resulted in the use of a wider and more conservative range of velocities in the 2005 analyses. The Seismic Boreholes Project was designed to directly measure the velocities and velocity contrasts in the basalts and sediments below the WTP, reanalyze the ground motion response, and assess the level of conservatism in the 2005 seismic design criteria

  15. Beyond-Design-Basis-Accidents Passive Containment-Cooling Spray System

    SciTech Connect (OSTI)

    Karameldin, Aly; Temraz, Hassan M. Elsawy; Ibrahim, Nady Attia [Atomic Energy Authority (Egypt)

    2001-10-15

    The proposed safety feature considered in this study aims to increase the safety margins of nuclear power plants by proposed water tanks located inside or outside the upper zone of the containment to be utilized for (a) residual heat removal of the reactor in case of station blackout or in case of normal reactor shutdown and (b) beyond-design-basis accidents, in which core melt and debris-concrete interaction take place, associated with accumulative containment pressure increase and partial loss of the active systems. The proposed passive containment system can be implemented by a special mechanism, which can allow the pressurization of the water in the tanks and therefore can enable an additional spray system to start in case of increasing the containment pressure over a certain value just below the design pressure. A conservative case study is that of a Westinghouse 3411-MW(thermal) power station, where the proposed passive containment cooling spray system (PCCSS) will start at a pressure of 6 bars and terminate at a pressure of 3 bars. A one-dimensional lumped model is postulated to describe the thermal and hydraulic process behavior inside the containment after a beyond-design-basis accident. The considered parameters are the spray mass flow rate, the initial droplet diameters, fuel-cooling time, and the ultimate containment pressure. The overall heat and mass balance inside the containment are carried out, during both the containment depressurization (by the spraying system) and pressurization (by the residual energies). The results show that the design of the PCCSS is viable and has a capability to maintain the containment below the design pressure passively for the required grace period of 72 h. Design curves of the proposed PCCSS indicate the effect of the spray flow rate and cooling time on the total sprayed volume during the grace period of 72 h. From these curves it can be concluded that for the grace period of 72 h, the required tank volumes are 3800

  16. Simulation of a beyond design-basis-accident with RELAP5/MOD3.1

    SciTech Connect (OSTI)

    Banati, J.

    1995-09-01

    This paper summarizes the results of analyses, parametric and sensitivity studies, performed using the RELAP5/MOD3.1 computer code for the 4th IAEA Standard Problem Exercise (SPE-4). The test, conducted on the PMK-2 facility in Budapest, involved simulation of a Small Break Loss Of Coolant Accident (SBLOCA) with a 7.4% break in the cold leg of a VVER-440 type pressurized water reactor. According to the scenario, the unavailability of the high pressure injection system led to a beyond design basis accident. For prevention of core damage, secondary side bleed-and-feed accident management measures were applied. A brief description of the PMK-2 integral type test facility is presented, together with the profile and some key phenomenological aspects of this particular experiment. Emphasis is placed on the ability of the code to predict the main trends observed in the test and thus, an assessment is given for the code capabilities to represent the system transient.

  17. Solution to PDEs using radial basis function finite-differences (RBF-FD) on multiple GPUs

    SciTech Connect (OSTI)

    Bollig, Evan F.; Flyer, Natasha; Erlebacher, Gordon

    2012-08-30

    This paper presents parallelization strategies for the radial basis function-finite difference (RBF-FD) method. As a generalized finite differencing scheme, the RBF-FD method functions without the need for underlying meshes to structure nodes. It offers high-order accuracy approximation and scales as O(N) per time step, with N being with the total number of nodes. To our knowledge, this is the first implementation of the RBF-FD method to leverage GPU accelerators for the solution of PDEs. Additionally, this implementation is the first to span both multiple CPUs and multiple GPUs. OpenCL kernels target the GPUs and inter-processor communication and synchronization is managed by the Message Passing Interface (MPI). We verify our implementation of the RBF-FD method with two hyperbolic PDEs on the sphere, and demonstrate up to 9x speedup on a commodity GPU with unoptimized kernel implementations. On a high performance cluster, the method achieves up to 7x speedup for the maximum problem size of 27,556 nodes.

  18. Technical Basis for Radiological Emergency Plan Annex for WTD Emergency Response Plan: West Point Treatment Plant

    SciTech Connect (OSTI)

    Hickey, Eva E.; Strom, Daniel J.

    2005-08-01

    Staff of the King County Wastewater Treatment Division (WTD) have concern about the aftermath of a radiological dispersion event (RDE) leading to the introduction of significant quantities of radioactive material into the combined sanitary and storm sewer system in King County, Washington. Radioactive material could come from the use of a radiological dispersion device (RDD). RDDs include "dirty bombs" that are not nuclear detonations but are explosives designed to spread radioactive material (National Council on Radiation Protection and Measurements (NCRP) 2001). Radioactive material also could come from deliberate introduction or dispersion of radioactive material into the environment, including waterways and water supply systems. This document, Volume 3 of PNNL-15163 is the technical basis for the Annex to the West Point Treatment Plant (WPTP) Emergency Response Plan related to responding to a radiological emergency at the WPTP. The plan primarily considers response to radioactive material that has been introduced in the other combined sanitary and storm sewer system from a radiological dispersion device, but is applicable to any accidental or deliberate introduction of materials into the system.

  19. The genetic basis of energy conservation in the sulfate-reducing bacterium Desulfovibrio alaskensis G20

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Price, Morgan N.; Ray, Jayashree; Wetmore, Kelly M.; Kuehl, Jennifer V.; Bauer, Stefan; Deutschbauer, Adam M.; Arkin, Adam P.

    2014-10-31

    Sulfate-reducing bacteria play major roles in the global carbon and sulfur cycles, but it remains unclear how reducing sulfate yields energy. To determine the genetic basis of energy conservation, we measured the fitness of thousands of pooled mutants of Desulfovibrio alaskensis G20 during growth in 12 different combinations of electron donors and acceptors. We show that ion pumping by the ferredoxin:NADH oxidoreductase Rnf is required whenever substrate-level phosphorylation is not possible. The uncharacterized complex Hdr/flox-1 (Dde_1207:13) is sometimes important alongside Rnf and may perform an electron bifurcation to generate more reduced ferredoxin from NADH to allow further ion pumping. Similarly,more » during the oxidation of malate or fumarate, the electron-bifurcating transhydrogenase NfnAB-2 (Dde_1250:1) is important and may generate reduced ferredoxin to allow additional ion pumping by Rnf. During formate oxidation, the periplasmic [NiFeSe] hydrogenase HysAB is required, which suggests that hydrogen forms in the periplasm, diffuses to the cytoplasm, and is used to reduce ferredoxin, thus providing a substrate for Rnf. We found that during hydrogen utilization, the transmembrane electron transport complex Tmc is important and may move electrons from the periplasm into the cytoplasmic sulfite reduction pathway. Finally, mutants of many other putative electron carriers have no clear phenotype, which suggests that they are not important under our growth conditions, although we cannot rule out genetic redundancy.« less

  20. Technical Basis for Certification of Seismic Design Criteria for the Waste Treatment Plant, Hanford, Washington

    SciTech Connect (OSTI)

    Brouns, Thomas M.; Rohay, Alan C.; Youngs, Robert R.; Costantino, Carl J.; Miller, Lewis F.

    2008-02-28

    In August 2007, Secretary of Energy Samuel W. Bodman approved the final seismic and ground motion criteria for the Waste Treatment and Immobilization Plant (WTP) at the Department of Energys (DOE) Hanford Site. Construction of the WTP began in 2002 based on seismic design criteria established in 1999 and a probabilistic seismic hazard analysis completed in 1996. The design criteria were re-evaluated in 2005 to address questions from the Defense Nuclear Facilities Safety Board (DNFSB), resulting in an increase by up to 40% in the seismic design basis. DOE announced in 2006 the suspension of construction on the pretreatment and high-level waste vitrification facilities within the WTP to validate the design with more stringent seismic criteria. In 2007, the U.S. Congress mandated that the Secretary of Energy certify the final seismic and ground motion criteria prior to expenditure of funds on construction of these two facilities. With the Secretarys approval of the final seismic criteria this past summer, DOE authorized restart of construction of the pretreatment and high-level waste vitrification facilities.

  1. Micrometer-scale fabrication of complex three dimensional lattice + basis structures in silicon

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Burckel, D. Bruce; Resnick, Paul J.; Finnegan, Patrick S.; Sinclair, Michael B.; Davids, Paul S.

    2015-01-01

    A complementary metal oxide semiconductor (CMOS) compatible version of membrane projection lithography (MPL) for fabrication of micrometer-scale three-dimensional structures is presented. The approach uses all inorganic materials and standard CMOS processing equipment. In a single layer, MPL is capable of creating all 5 2D-Bravais lattices. Furthermore, standard semiconductor processing steps can be used in a layer-by-layer approach to create fully three dimensional structures with any of the 14 3D-Bravais lattices. The unit cell basis is determined by the projection of the membrane pattern, with many degrees of freedom for defining functional inclusions. Here we demonstrate several unique structural motifs, andmorecharacterize 2D arrays of unit cells with split ring resonators in a silicon matrix. The structures exhibit strong polarization dependent resonances and, for properly oriented split ring resonators (SRRs), coupling to the magnetic field of a normally incident transverse electromagnetic wave, a response unique to 3D inclusions.less

  2. Using compliance audits as the basis for developing an effective mechanical integrity program

    SciTech Connect (OSTI)

    Kiihne, E.J.; Mannan, M. [RMT/Jones and Neuse, Inc., Austin, TX (United States)

    1996-08-01

    The OSHA Process Safety Management (PSM) rule requires all covered facilities to conduct a compliance audit every three years. In addition, all the audit findings must be resolved within a reasonable time period. The process industry as a whole is lagging behind in compliance with mechanical integrity program requirements as demonstrated by the high number of OSHA citations issued to-date on mechanical integrity-related issues. This paper analyzes the findings of several PSM compliance audits and develops recommendations for developing effective mechanical integrity programs. The six explicit requirements of mechanical integrity, i.e. covered equipment, written procedures, training, inspection and testing, equipment deficiencies, and quality assurance are analyzed in the following manner: Number of OSHA citations in mechanical integrity and the distribution of these citations between the six specific requirements. Comparison of the OSHA citations with the audit findings from PSM compliance audits conducted by the authors. Using the conclusions from the OSHA citations and PSM compliance audits as a basis for developing effective mechanical integrity programs.

  3. Application of Radial Basis Functional Link Networks to Exploration for Proterozoic Mineral Deposits in Central Iran

    SciTech Connect (OSTI)

    Behnia, Pouran [Geological Survey of Iran, Geomatics Department (Iran, Islamic Republic of)], E-mail: pouranb@yahoo.com

    2007-06-15

    The metallogeny of Central Iran is characterized mainly by the presence of several iron, apatite, and uranium deposits of Proterozoic age. Radial Basis Function Link Networks (RBFLN) were used as a data-driven method for GIS-based predictive mapping of Proterozoic mineralization in this area. To generate the input data for RBFLN, the evidential maps comprising stratigraphic, structural, geophysical, and geochemical data were used. Fifty-eight deposits and 58 'nondeposits' were used to train the network. The operations for the application of neural networks employed in this study involve both multiclass and binary representation of evidential maps. Running RBFLN on different input data showed that an increase in the number of evidential maps and classes leads to a larger classification sum of squared error (SSE). As a whole, an increase in the number of iterations resulted in the improvement of training SSE. The results of applying RBFLN showed that a successful classification depends on the existence of spatially well distributed deposits and nondeposits throughout the study area.

  4. Natural micropolymorphism in human leukocyte antigens provides a basis for genetic control of antigen recognition

    SciTech Connect (OSTI)

    Archbold, Julia K.; Macdonald, Whitney A.; Gras, Stephanie; Ely, Lauren K.; Miles, John J.; Bell, Melissa J.; Brennan, Rebekah M.; Beddoe, Travis; Wilce, Matthew C.J.; Clements, Craig S.; Purcell, Anthony W.; McCluskey, James; Burrows, Scott R.; Rossjohn, Jamie

    2009-07-10

    Human leukocyte antigen (HLA) gene polymorphism plays a critical role in protective immunity, disease susceptibility, autoimmunity, and drug hypersensitivity, yet the basis of how HLA polymorphism influences T cell receptor (TCR) recognition is unclear. We examined how a natural micropolymorphism in HLA-B44, an important and large HLA allelic family, affected antigen recognition. T cell-mediated immunity to an Epstein-Barr virus determinant (EENLLDFVRF) is enhanced when HLA-B*4405 was the presenting allotype compared with HLA-B*4402 or HLA-B*4403, each of which differ by just one amino acid. The micropolymorphism in these HLA-B44 allotypes altered the mode of binding and dynamics of the bound viral epitope. The structure of the TCR-HLA-B*4405EENLLDFVRF complex revealed that peptide flexibility was a critical parameter in enabling preferential engagement with HLA-B*4405 in comparison to HLA-B*4402/03. Accordingly, major histocompatibility complex (MHC) polymorphism can alter the dynamics of the peptide-MHC landscape, resulting in fine-tuning of T cell responses between closely related allotypes.

  5. APPARATUS FOR THE MASS ANALYSIS OF PLASMA ON A CONTINUOUS BASIS

    DOE Patents [OSTI]

    Neidigh, R.V.

    1963-07-01

    An apparatus for the mass analysis of plasmas on a continuous basis is described. The apparatus comprises a pair of parallel electrodes in a tubular member which serve as a velocity-selecting region for ions drawn by an accelerating potential through a tapered nose cone affixed to the tubular member. The magnetic force and electrostatic forces in the velocity-selecting region are made equal and opposite in direction to prevent the ionic species from striking either of the electrodes as they traverse the region. A pair of parallel plates is positioned within the tubular member and in alignment with the electrodes, but displaced slightly so as not to be seen by direct light coming through the entrance slit of the nose cone, and one of these plates serves as a collector plate. This collector plate is coupled to the vertical amplifier of an oscilloscope or other recorder to provide a continuous indication of the ionic coinposition of the plasma under analysis. ( DELTA EC)

  6. Application of the MELCOR code to design basis PWR large dry containment analysis.

    SciTech Connect (OSTI)

    Phillips, Jesse; Notafrancesco, Allen (USNRC, Office of Nuclear Regulatory Research, Rockville, MD); Tills, Jack Lee (Jack Tills & Associates, Inc., Sandia Park, NM)

    2009-05-01

    The MELCOR computer code has been developed by Sandia National Laboratories under USNRC sponsorship to provide capability for independently auditing analyses submitted by reactor manufactures and utilities. MELCOR is a fully integrated code (encompassing the reactor coolant system and the containment building) that models the progression of postulated accidents in light water reactor power plants. To assess the adequacy of containment thermal-hydraulic modeling incorporated in the MELCOR code for application to PWR large dry containments, several selected demonstration designs were analyzed. This report documents MELCOR code demonstration calculations performed for postulated design basis accident (DBA) analysis (LOCA and MSLB) inside containment, which are compared to other code results. The key processes when analyzing the containment loads inside PWR large dry containments are (1) expansion and transport of high mass/energy releases, (2) heat and mass transfer to structural passive heat sinks, and (3) containment pressure reduction due to engineered safety features. A code-to-code benchmarking for DBA events showed that MELCOR predictions of maximum containment loads were equivalent to similar predictions using a qualified containment code known as CONTAIN. This equivalency was found to apply for both single- and multi-cell containment models.

  7. Modular High Temperature Gas-Cooled Reactor Safety Basis and Approach

    SciTech Connect (OSTI)

    David Petti; Jim Kinsey; Dave Alberstein

    2014-01-01

    Various international efforts are underway to assess the safety of advanced nuclear reactor designs. For example, the International Atomic Energy Agency has recently held its first Consultancy Meeting on a new cooperative research program on high temperature gas-cooled reactor (HTGR) safety. Furthermore, the Generation IV International Forum Reactor Safety Working Group has recently developed a methodology, called the Integrated Safety Assessment Methodology, for use in Generation IV advanced reactor technology development, design, and design review. A risk and safety assessment white paper is under development with respect to the Very High Temperature Reactor to pilot the Integrated Safety Assessment Methodology and to demonstrate its validity and feasibility. To support such efforts, this information paper on the modular HTGR safety basis and approach has been prepared. The paper provides a summary level introduction to HTGR history, public safety objectives, inherent and passive safety features, radionuclide release barriers, functional safety approach, and risk-informed safety approach. The information in this paper is intended to further the understanding of the modular HTGR safety approach. The paper gives those involved in the assessment of advanced reactor designs an opportunity to assess an advanced design that has already received extensive review by regulatory authorities and to judge the utility of recently proposed new methods for advanced reactor safety assessment such as the Integrated Safety Assessment Methodology.

  8. Human-system Interfaces to Automatic Systems: Review Guidance and Technical Basis

    SciTech Connect (OSTI)

    OHara, J.M.; Higgins, J.C.

    2010-01-31

    Automation has become ubiquitous in modern complex systems and commercial nuclear power plants are no exception. Beyond the control of plant functions and systems, automation is applied to a wide range of additional functions including monitoring and detection, situation assessment, response planning, response implementation, and interface management. Automation has become a 'team player' supporting plant personnel in nearly all aspects of plant operation. In light of the increasing use and importance of automation in new and future plants, guidance is needed to enable the NRC staff to conduct safety reviews of the human factors engineering (HFE) aspects of modern automation. The objective of the research described in this report was to develop guidance for reviewing the operator's interface with automation. We first developed a characterization of the important HFE aspects of automation based on how it is implemented in current systems. The characterization included five dimensions: Level of automation, function of automation, modes of automation, flexibility of allocation, and reliability of automation. Next, we reviewed literature pertaining to the effects of these aspects of automation on human performance and the design of human-system interfaces (HSIs) for automation. Then, we used the technical basis established by the literature to develop design review guidance. The guidance is divided into the following seven topics: Automation displays, interaction and control, automation modes, automation levels, adaptive automation, error tolerance and failure management, and HSI integration. In addition, we identified insights into the automaton design process, operator training, and operations.

  9. TECHNICAL BASIS FOR EVALUATING SURFACE BARRIERS TO PROTECT GROUNDWATER FROM DEEP VADOSE ZONE CONTAMINATION

    SciTech Connect (OSTI)

    FAYER JM; FREEDMAN VL; WARD AL; CHRONISTER GB

    2010-02-24

    tasks to achieve those outcomes. Full understanding of contaminant behavior in the deep vadose zone is constrained by four key data gaps: limited access; limited data; limited time; and the lack of an accepted predictive capability for determining whether surface barriers can effectively isolate deep vadose zone contaminants. Activities designed to fill these data gaps need to have these outcomes: (1) common evaluation methodology that provides a clear, consistent, and defensible basis for evaluating groundwater impacts caused by placement of a surface barrier above deep vadose zone contamination; (2) deep vadose zone data that characterize the lithology, the spatial distribution of moisture and contaminants, the physical, chemical, and biological process that affect the mobility of each contaminant, and the impacts to the contaminants following placement of a surface barrier; (3) subsurface monitoring to provide subsurface characterization of initial conditions and changes that occur during and following remediation activities; and (4) field observations that span years to decades to validate the evaluation methodology. A set of six proposed tasks was identified to provide information needed to address the above outcomes. The proposed tasks are: (1) Evaluation Methodology - Develop common evaluation methodology that will provide a clear, consistent, and defensible basis for evaluating groundwater impacts caused by placement of a surface barrier above deep vadose zone contamination. (2) Case Studies - Conduct case studies to demonstrate the applicability ofthe common evaluation methodology and provide templates for subsequent use elsewhere. Three sites expected to have conditions that would yield valuable information and experience pertinent to deep vadose zone contamination were chosen to cover a range of conditions. The sites are BC Cribs and Trenches, U Plant Cribs, and the T Farm Interim Cover. (3) Subsurface Monitoring Technologies - Evaluate minimally invasive

  10. Nuclear-Renewable Hybrid System Economic Basis for Electricity, Fuel, and Hydrogen

    SciTech Connect (OSTI)

    Charles Forsberg; Steven Aumeier

    2014-04-01

    Concerns about climate change and altering the ocean chemistry are likely to limit the use of fossil fuels. That implies a transition to a low-carbon nuclear-renewable electricity grid. Historically variable electricity demand was met using fossil plants with low capital costs, high operating costs, and substantial greenhouse gas emissions. However, the most easily scalable very-low-emissions generating options, nuclear and non-dispatchable renewables (solar and wind), are capital-intensive technologies with low operating costs that should operate at full capacities to minimize costs. No combination of fully-utilized nuclear and renewables can meet the variable electricity demand. This implies large quantities of expensive excess generating capacity much of the time. In a free market this results in near-zero electricity prices at times of high nuclear renewables output and low electricity demand with electricity revenue collapse. Capital deployment efficiency—the economic benefit derived from energy systems capital investment at a societal level—strongly favors high utilization of these capital-intensive systems, especially if low-carbon nuclear renewables are to replace fossil fuels. Hybrid energy systems are one option for better utilization of these systems that consumes excess energy at times of low prices to make some useful product.The economic basis for development of hybrid energy systems is described for a low-carbon nuclear renewable world where much of the time there are massivequantities of excess energy available from the electric sector.Examples include (1) high-temperature electrolysis to generate hydrogen for non-fossil liquid fuels, direct use as a transport fuel, metal reduction, etc. and (2) biorefineries.Nuclear energy with its concentrated constant heat output may become the enabling technology for economically-viable low-carbon electricity grids because hybrid nuclear systems may provide an economic way to produce dispatachable variable

  11. Structural basis for effectiveness of siderophore-conjugated monocarbams against clinically relevant strains of Pseudomonas aeruginosa

    SciTech Connect (OSTI)

    Han, Seungil; Zaniewski, Richard P.; Marr, Eric S.; Lacey, Brian M.; Tomaras, Andrew P.; Evdokimov, Artem; Miller, J. Richard; Shanmugasundaram, Veerabahu

    2012-02-08

    Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that causes nosocomial infections for which there are limited treatment options. Penicillin-binding protein PBP3, a key therapeutic target, is an essential enzyme responsible for the final steps of peptidoglycan synthesis and is covalently inactivated by {beta}-lactam antibiotics. Here we disclose the first high resolution cocrystal structures of the P. aeruginosa PBP3 with both novel and marketed {beta}-lactams. These structures reveal a conformational rearrangement of Tyr532 and Phe533 and a ligand-induced conformational change of Tyr409 and Arg489. The well-known affinity of the monobactam aztreonam for P. aeruginosa PBP3 is due to a distinct hydrophobic aromatic wall composed of Tyr503, Tyr532, and Phe533 interacting with the gem-dimethyl group. The structure of MC-1, a new siderophore-conjugated monocarbam complexed with PBP3 provides molecular insights for lead optimization. Importantly, we have identified a novel conformation that is distinct to the high-molecular-weight class B PBP subfamily, which is identifiable by common features such as a hydrophobic aromatic wall formed by Tyr503, Tyr532, and Phe533 and the structural flexibility of Tyr409 flanked by two glycine residues. This is also the first example of a siderophore-conjugated triazolone-linked monocarbam complexed with any PBP. Energetic analysis of tightly and loosely held computed hydration sites indicates protein desolvation effects contribute significantly to PBP3 binding, and analysis of hydration site energies allows rank ordering of the second-order acylation rate constants. Taken together, these structural, biochemical, and computational studies provide a molecular basis for recognition of P. aeruginosa PBP3 and open avenues for future design of inhibitors of this class of PBPs.

  12. Human System Simulation in Support of Human Performance Technical Basis at NPPs

    SciTech Connect (OSTI)

    David Gertman; Katya Le Blanc; alan mecham; william phoenix; Magdy Tawfik; Jeffrey Joe

    2010-06-01

    This paper focuses on strategies and progress toward establishing the Idaho National Laboratorys (INLs) Human Systems Simulator Laboratory at the Center for Advanced Energy Studies (CAES), a consortium of Idaho State Universities. The INL is one of the National Laboratories of the US Department of Energy. One of the first planned applications for the Human Systems Simulator Laboratory is implementation of a dynamic nuclear power plant simulation (NPP) where studies of operator workload, situation awareness, performance and preference will be carried out in simulated control rooms including nuclear power plant control rooms. Simulation offers a means by which to review operational concepts, improve design practices and provide a technical basis for licensing decisions. In preparation for the next generation power plant and current government and industry efforts in support of light water reactor sustainability, human operators will be attached to a suite of physiological measurement instruments and, in combination with traditional Human Factors Measurement techniques, carry out control room tasks in simulated advanced digital and hybrid analog/digital control rooms. The current focus of the Human Systems Simulator Laboratory is building core competence in quantitative and qualitative measurements of situation awareness and workload. Of particular interest is whether introduction of digital systems including automated procedures has the potential to reduce workload and enhance safety while improving situation awareness or whether workload is merely shifted and situation awareness is modified in yet to be determined ways. Data analysis is carried out by engineers and scientists and includes measures of the physical and neurological correlates of human performance. The current approach supports a user-centered design philosophy (see ISO 13407 Human Centered Design Process for Interactive Systems, 1999) wherein the context for task performance along with the

  13. NNSA?s Computing Strategy, Acquisition Plan, and Basis for Computing Time Allocation

    SciTech Connect (OSTI)

    Nikkel, D J

    2009-07-21

    This report is in response to the Omnibus Appropriations Act, 2009 (H.R. 1105; Public Law 111-8) in its funding of the National Nuclear Security Administration's (NNSA) Advanced Simulation and Computing (ASC) Program. This bill called for a report on ASC's plans for computing and platform acquisition strategy in support of stockpile stewardship. Computer simulation is essential to the stewardship of the nation's nuclear stockpile. Annual certification of the country's stockpile systems, Significant Finding Investigations (SFIs), and execution of Life Extension Programs (LEPs) are dependent on simulations employing the advanced ASC tools developed over the past decade plus; indeed, without these tools, certification would not be possible without a return to nuclear testing. ASC is an integrated program involving investments in computer hardware (platforms and computing centers), software environments, integrated design codes and physical models for these codes, and validation methodologies. The significant progress ASC has made in the past derives from its focus on mission and from its strategy of balancing support across the key investment areas necessary for success. All these investment areas must be sustained for ASC to adequately support current stockpile stewardship mission needs and to meet ever more difficult challenges as the weapons continue to age or undergo refurbishment. The appropriations bill called for this report to address three specific issues, which are responded to briefly here but are expanded upon in the subsequent document: (1) Identify how computing capability at each of the labs will specifically contribute to stockpile stewardship goals, and on what basis computing time will be allocated to achieve the goal of a balanced program among the labs. (2) Explain the NNSA's acquisition strategy for capacity and capability of machines at each of the labs and how it will fit within the existing budget constraints. (3) Identify the technical

  14. Physics Basis for the Advanced Tokamak Fusion Power Plant ARIES-AT

    SciTech Connect (OSTI)

    S.C. Jardin; C.E. Kessel; T.K. Mau; R.L. Miller; F. Najmabadi; V.S. Chan; M.S. Chu; R. LaHaye; L.L. Lao; T.W. Petrie; P. Politzer; H.E. St. John; P. Snyder; G.M. Staebler; A.D. Turnbull; W.P. West

    2003-10-07

    The advanced tokamak is considered as the basis for a fusion power plant. The ARIES-AT design has an aspect ratio of A always equal to R/a = 4.0, an elongation and triangularity of kappa = 2.20, delta = 0.90 (evaluated at the separatrix surface), a toroidal beta of beta = 9.1% (normalized to the vacuum toroidal field at the plasma center), which corresponds to a normalized beta of bN * 100 x b/(I(sub)P(MA)/a(m)B(T)) = 5.4. These beta values are chosen to be 10% below the ideal-MHD stability limit. The bootstrap-current fraction is fBS * I(sub)BS/I(sub)P = 0.91. This leads to a design with total plasma current I(sub)P = 12.8 MA, and toroidal field of 11.1 T (at the coil edge) and 5.8 T (at the plasma center). The major and minor radii are 5.2 and 1.3 m, respectively. The effects of H-mode edge gradients and the stability of this configuration to non-ideal modes is analyzed. The current-drive system consists of ICRF/FW for on-axis current drive and a lower-hybrid system for off-axis. Tran sport projections are presented using the drift-wave based GLF23 model. The approach to power and particle exhaust using both plasma core and scrape-off-layer radiation is presented.

  15. Improving Remedial Effectiveness at U.S. Department of Energy through Optimization Review and Performance Basis

    SciTech Connect (OSTI)

    Moore, B.A.

    2008-07-01

    The U.S. Department of Energy (DOE), Office of Environmental Management (EM) conducted its first Remediation Process Optimization (RPO) review in 2004, following the methodology and guidance issued by the Interstate Technology and Regulatory Council (ITRC). The intent of this paper is to briefly summarize the following: (1) the overall benefits of the review process toward improving remedial effectiveness and efficiency at DOE, (2) the types and objectives of completed reviews, and (3) how RPO facilitates technology transfer and is complementary to performance-based environmental management (PBEM). Contract reform began in 1993, at the U.S. Department of Energy (DOE) as a result of the Government Performance and Results Act, followed by recommendations from oversight groups. The precedence of using management and operating (M and O) contracts at DOE facilities, exempt from open competition by definition, shifted to performance basis. Since 1994, DOE has competed over 70% of its then existing M and O contracts; most now contain specific performance objectives, measures, and targets that focus on results in mission critical areas. In 2001, DOE's Office of Environmental Management (EM) integrated performance-based contracting as a core business process. EM resolved to manage cleanup as a project, encourage innovative contracting strategies, as well as incentives to accelerate risk reduction and cleanup. DOE's efforts to implement performance-based environmental management (PBEM) were further realized by establishing a centralized Office of Acquisitions and the Consolidated Business Center, which support all EM procurements. In 2004, EM began conducting Remediation Process Optimization (RPO) reviews at select field sites, following the methodology published in the 2004 ITRC guidance. To date, EM has completed nine RPO reviews, including: pump and treat system optimization, monitoring program optimization, in situ barrier performance improvement, remedial design review

  16. Drain Tank Information for Developing Design Basis of the Preliminary Design

    SciTech Connect (OSTI)

    Ferrada, Juan J

    2012-02-01

    Tokamak Cooling Water System (TCWS) drain tanks (DTs) serve two functions: normal operation and safety operation. Normal DTs are used for regular maintenance operations when draining is necessary. Safety DTs are used to receive the water leaked into the Vacuum Vessel (VV) after an in-vessel loss of coolant accident (LOCA) event. The preliminary design of the DTs shall be based on the information provided by this document. The capacity of the normal DTs is estimated based on the internal volume of in-vessel components [e.g., First Wall/Blanket (FW/BLK) and Divertor (DIV)]; Neutral Beam Injector (NBI) components; and TCWS piping, heat exchangers, electric heaters, pump casing, pressurizers, and valves. Water volumes have been updated based on 2004-design information, changes adopted because of approved Project Change Requests (PCRs), and data verification by US ITER and AREVA Federal Services, the US ITER A and E Company. Two tanks will store water from normal draining operations of the FW/BLK and DIV Primary Heat Transfer Systems (PHTSs). One tank will store water from normal draining operations of the NBI PHTS. The capacity of the safety DTs is based on analysis of a design basis accident: a large leak from in-vessel components. There are two safety DTs that will receive water from a VV LOCA event and drainage from the VV, as needed. In addition, there is one sump tank for the DIV that will be used for collecting drain water from the draining and drying processes and specifically for draining the DIV system as the DIV cassette lines are at a lower elevation than the DT connection point. Information documented in this report must be refined and verified during the preliminary design of the DTs, and there are several aspects to be considered to complete the preliminary design. Input to these design considerations is discussed in this report and includes, but is not limited to, water inventory; operating procedures/maintenance; Failure Modes and Effects Analysis (FMEA

  17. Drain Tank Information for Developing Design Basis of the Preliminary Design - R00

    SciTech Connect (OSTI)

    Ferrada, Juan J

    2011-01-01

    Tokamak Cooling Water System (TCWS) drain tanks (DTs) serve two functions: normal operation and safety operation. Normal DTs are used for regular maintenance operations when draining is necessary. Safety DTs are used to receive the water leaked into the Vacuum Vessel (VV) after an in-vessel loss of cooling accident (LOCA) event. The preliminary design of the DTs shall be based on the information provided by this document. The capacity of the normal DTs is estimated based on the internal volume of in-vessel components [e.g., First Wall/Blanket (FW/BLK) and Divertor (DIV)], Neutral Beam Injector (NBI) components, and TCWS piping, heat exchangers, electric heaters, pump casing, pressurizers, and valves. Water volumes have been updated based on 2004 design information, changes adopted because of approved Project Change Requests (PCRs), and data verification by U.S. ITER. Two tanks will store water from normal draining operations of the FW/BLK and DIV Primary Heat Transfer Systems (PHTSs). One tank will store water from normal draining operations of the NBI PHTS. The capacity of the safety DTs is based on analysis of a design-basis accident:1 a large leak from in-vessel components. There are two safety DTs that will receive water from a VV LOCA event and drainage from the VV, as needed. In addition, there is one sump tank for the DIV that will be used for collecting drain water from the draining and drying processes and specifically for draining the DIV system as the DIV cassettes lines are at a lower elevation than the DT connection point. Information documented in this report must be refined and verified during the preliminary design of the DTs, and there are several aspects to be considered to complete the preliminary design. Input to these design considerations is discussed in this report and includes, but is not limited to, water inventory; operating procedures/maintenance; Failure Modes and Effects Analysis (FMEA); tank layout anddimensions, including design

  18. High integrity software for nuclear power plants: Candidate guidelines, technical basis and research needs. Executive summary: Volume 1

    SciTech Connect (OSTI)

    Seth, S.; Bail, W.; Cleaves, D.; Cohen, H.; Hybertson, D.; Schaefer, C.; Stark, G.; Ta, A.; Ulery, B.

    1995-06-01

    The work documented in this report was performed in support of the US Nuclear Regulatory Commission to examine the technical basis for candidate guidelines that could be considered in reviewing and evaluating high integrity computer software used in the safety systems of nuclear power plants. The framework for the work consisted of the following software development and assurance activities: requirements specification; design; coding; verification and validation, including static analysis and dynamic testing; safety analysis; operation and maintenance; configuration management; quality assurance; and planning and management. Each activity (framework element) was subdivided into technical areas (framework subelements). The report describes the development of approximately 200 candidate guidelines that span the entire range of software life-cycle activities; the assessment of the technical basis for those candidate guidelines; and the identification, categorization and prioritization of research needs for improving the technical basis. The report has two volumes: Volume 1, Executive Summary, includes an overview of the framework and of each framework element, the complete set of candidate guidelines, the results of the assessment of the technical basis for each candidate guideline, and a discussion of research needs that support the regulatory function; Volume 2 is the main report.

  19. Independent Oversight Review of the Los Alamos National Laboratory Transuranic Waste Facility Safety Basis and Design Development, July 2014

    Office of Environmental Management (EM)

    Los Alamos National Laboratory Transuranic Waste Facility Safety Basis and Design Development July 2014 Office of Nuclear Safety and Environmental Assessments Office of Environment, Safety and Health Assessments Office of Independent Enterprise Assessments U.S. Department of Energy Table of Contents 1.0 Purpose ................................................................................................................................................ 1 2.0 Scope ...

  20. Review of the Savannah River Site Salt Waste Processing Facility Safety Basis and Design Development, August 2013

    Office of Environmental Management (EM)

    Savannah River Site Salt Waste Processing Facility Safety Basis and Design Development May 2011 August 2013 Office of Safety and Emergency Management Evaluations Office of Enforcement and Oversight Office of Health, Safety and Security U.S. Department of Energy Table of Contents 1.0 Purpose.................................................................................................................................................... 1 2.0 Background

  1. Long-Baseline Neutrino Experiment (LBNE)Water Cherenkov Detector Basis of Estimate Forms and Backup Documentation LBNE Far Site Internal Review (December 6-9, 2011)

    SciTech Connect (OSTI)

    Stewart J.; Diwan, M.; Dolph, J.; Novakova, P.; Sharma, R.; Stewart, J.; Viren, B.; Russo, T.; Kaducak, M.; Mantsch, P.; Paulos, B.; Feyzi, F.; Sullivan, G.; Bionta, R.; Fowler, J.; Warner, D.; Bahowick, S.; Van Berg, R.; Kearns, E.; Hazen, E.; Sinnis, G.; Sanchez, M.

    2011-12-09

    Basis of Estimate (BOE) forms and backup documentation developed for the Water Cherenkov Detector (WCD) option for the far detector of the Long Baseline Neutrino Experiment (LBNE)

  2. Evaluation of the Scaling of the APEX-1000 Test Facility to AP1000 for Design Basis Events

    SciTech Connect (OSTI)

    Wright, Richard F.; Gagnon, Andre; Skinner, Jesse; Groome, John

    2004-07-01

    The AP1000 is a 1000 MWe advanced nuclear power plant that uses passive safety features to enhance plant safety and to provide significant and measurable improvements in plant simplification, reliability, investment protection and plant costs. The AP1000 relies heavily on the 600 MWe AP600 which received design certification in 1999. A critical part of the AP600 design certification process involved the testing of the passive safety systems. A one-fourth height, one-fourth pressure test facility, APEX-600, was constructed at the Oregon State University to study design basis events, and to provide a body of data to be used to validate the computer models used to analyze the AP600. This facility was extensively modified to reflect the design changes for AP1000 including higher power in the electrically heated rods representing the reactor core, and changes in the size of the pressurizer, core makeup tanks and automatic depressurization system. Several design basis events have been simulated at APEX-1000 including double-ended direct vessel injection (DEDVI) line break, 2-inch cold leg break, and inadvertent actuation of the automatic depressurization system. These events were analyzed as part of the AP1000 safety analysis using the NOTRUMP computer code. Applying the scaling factors that were used to size the APEX-1000 facility, the test results can be compared to the NOTRUMP plant simulations. These comparisons indicate the similarity between the design basis events in AP1000 and APEX-1000, the adequacy of the scaling of APEX-1000, and the applicability of the NOTRUMP code. (authors)

  3. Basis to demonstrate compliance with the National Emission Standards for Hazardous Air Pollutants for the Stand-off Experiments Range

    SciTech Connect (OSTI)

    Michael Sandvig

    2011-01-01

    The purpose of this report is to provide the basis and the documentation to demonstrate general compliance with the National Emission Standard for Hazardous Air Pollutants (NESHAPS) 40 CFR 61 Subpart H, National Emission Standards for Emissions of Radionuclides Other Than Radon from Department of Energy Facilities, (the Standard) for outdoor linear accelerator operations at the Idaho National Laboratory (INL) Stand-off Experiments Range (SOX). The intent of this report is to inform and gain acceptance of this methodology from the governmental bodies regulating the INL.

  4. Refining and end use study of coal liquids. Topical report: Petroleum Refinery; Linear Programming Model; and Design Basis

    SciTech Connect (OSTI)

    1995-03-01

    A model was developed for use in the Bechtel PIMS (Process Industry Modeling System) linear programming software to simulate a generic Midwest (PADD II) petroleum refinery of the future. This ``petroleum-only`` version of the model establishes the size and complexity of the refinery after the year 2000 and prior to the introduction of coal liquids. It should be noted that no assumption has been made on when a plant can be built to produce coal liquids except that it will be after the year 2000. The year 2000 was chosen because it is the latest year where fuel property and emission standards have been set by the Environmental Protection Agency. It assumes the refinery has been modified to accept crudes that are heavier in gravity and higher in sulfur than today`s average crude mix. In addition, the refinery has also been modified to produce a product slate of transportation fuels of the future (i.e. 40% reformulated gasolines). This model will be used as a basis for determining the optimum scheme for processing coal liquids in a petroleum refinery. This report summarizes the design basis for this ``petroleum only`` LP refinery model. A report detailing the refinery configuration when coal liquids are processed will be provided at a later date.

  5. Tank waste characterization basis

    SciTech Connect (OSTI)

    Brown, T.M.

    1996-08-09

    This document describes the issues requiring characterization information, the process of determining high priority tanks to obtain information, and the outcome of the prioritization process. In addition, this document provides the reasoning for establishing and revising priorities and plans.

  6. design basis threat

    National Nuclear Security Administration (NNSA)

    training courses, and additional full-time instructors.

    • The shift to an "Elite Forces," or Tactical Response Force (TRF) model, to transform NNSA's protective forces...

    • Geochemistry Technical Basis Document

      SciTech Connect (OSTI)

      Benedict, Jr, F Christopher; Rose, Timothy P; Thomas, James M; Waddell, Richard; Jacobson, Roger

      2004-03-18

      This document presents a methodology whereby geochemical data can more effectively contribute to the development , calibration, and verification of groundwater flow and slute transport models for the Underground Test Area (UGTA) Project.

    • After Action Report:Idaho National Laboratory (INL) 2014 Multiple Facility Beyond Design Basis (BDBE) Evaluated Drill October 21, 2014

      SciTech Connect (OSTI)

      V. Scott Barnes

      2014-12-01

      On October 21, 2014, Idaho National Laboratory (INL), in coordination with local jurisdictions, and Department of Energy (DOE) Idaho Operations Office (DOE ID) conducted an evaluated drill to demonstrate the ability to implement the requirements of DOE O 151.1C, “Comprehensive Emergency Management System” when responding to a beyond design basis event (BDBE) scenario as outlined in the Office of Health, Safety, and Security Operating Experience Level 1 letter (OE-1: 2013-01). The INL contractor, Battelle Energy Alliance, LLC (BEA), in coordination with CH2M-WG Idaho, LLC (CWI), and Idaho Treatment Group LLC (ITG), successfully demonstrated appropriate response measures to mitigate a BDBE event that would impact multiple facilities across the INL while protecting the health and safety of personnel, the environment, and property. Offsite response organizations participated to demonstrate appropriate response measures.

    • Light Water Reactor Sustainability Program Technical Basis Guide Describing How to Perform Safety Margin Configuration Risk Management

      SciTech Connect (OSTI)

      Curtis Smith; James Knudsen; Bentley Harwood

      2013-08-01

      The INL has carried out a demonstration of the RISMC approach for the purpose of configuration risk management. We have shown how improved accuracy and realism can be achieved by simulating changes in risk as a function of different configurations in order to determine safety margins as the plant is modified. We described the various technical issues that play a role in these configuration-based calculations with the intent that future applications can take advantage of the analysis benefits while avoiding some of the technical pitfalls that are found for these types of calculations. Specific recommendations have been provided on a variety of topics aimed at improving the safety margin analysis and strengthening the technical basis behind the analysis process.

    • Photodetectors on the basis of Ge/Si(001) heterostructures grown by the hot-wire CVD technique

      SciTech Connect (OSTI)

      Shengurov, V. G. Chalkov, V. Yu.; Denisov, S. A.; Alyabina, N. A.; Guseinov, D. V.; Trushin, V. N.; Gorshkov, A. P.; Volkova, N. S.; Ivanova, M. M.; Kruglov, A. V.; Filatov, D. O.

      2015-10-15

      The fabrication of photodetectors for the wavelength range of communications λ = 1.3–1.55 µm on the basis of Ge/Si(001) heterostructures with thick (∼0.5 µm) Ge layers grown by the hot-wire technique at reduced growth temperatures (350°C) is reported. The single-crystal Ga layers are distinguished by a low density of threading dislocations (∼10{sup 5} cm{sup –2}). The photodetectors exhibit a rather high quantum efficiency (∼0.05 at λ = 1.5 µm and 300 K) at moderate reverse saturation current densities (∼10{sup –2} A cm{sup –2}). Thus, it is shown that the hot-wire technique offers promise for the formation of integrated photodetectors for the wavelength range of communications, especially in the case of limitations on the conditions of heat treatments.

    • Development of Site-Specific Soil Design Basis Earthquake (DBE) Parameters for the Integrated Waste Treatment Unit (IWTU)

      SciTech Connect (OSTI)

      Payne, Suzette

      2008-08-01

      Horizontal and vertical PC 3 (2,500 yr) Soil Design Basis Earthquake (DBE) 5% damped spectra, corresponding time histories, and strain-compatible soil properties were developed for the Integrated Waste Treatment Unit (IWTU). The IWTU is located at the Idaho Nuclear Technology and Engineering Center (INTEC) at the Idaho National Laboratory (INL). Mean and 84th percentile horizontal DBE spectra derived from site-specific site response analyses were evaluated for the IWTU. The horizontal and vertical PC 3 (2,500 yr) Soil DBE 5% damped spectra at the 84th percentile were selected for Soil Structure Interaction (SSI) analyses at IWTU. The site response analyses were performed consistent with applicable Department of Energy (DOE) Standards, recommended guidance of the Nuclear Regulatory Commission (NRC), American Society of Civil Engineers (ASCE) Standards, and recommendations of the Blue Ribbon Panel (BRP) and Defense Nuclear Facilities Safety Board (DNFSB).

    • Representation of a complex Green function on a real basis: Generalization to a three-body system

      SciTech Connect (OSTI)

      Li Tieniu; Shakeshaft, Robin; Piraux, Bernard

      2003-05-01

      We develop further a new method for employing a set of real basis functions to represent the Green function at energies in the continuum, without regard for the asymptotic boundary conditions. The method is based on the analyticity of the Green function with respect to its underlying time scale. The diagonalization of large matrices is unnecessary. Although a large complex symmetric linear system of equations must be solved, this can be done with high stability and efficiency by using a generalization of the Cholesky decomposition of real positive definite symmetric matrices. We present results of test applications to {sup 1}S-wave electron scattering from a hydrogen atom and photodetachment of the negative hydrogen ion. The extension from two- to three-body collisions entails the use of projection operators to distinguish different groups of asymptotic channels.

    • 105-K Basin Material Design Basis Feed Description for Spent Nuclear Fuel (SNF) Project Facilities VOL 1 Fuel

      SciTech Connect (OSTI)

      PACKER, M.J.

      1999-11-04

      Metallic uranium Spent Nuclear Fuel (SNF) is currently stored within two water filled pools, 105-KE Basin (KE Basin) and 105-KW Basin (KW Basin), at the United States Department of Energy (U.S. DOE) Hanford Site, in southeastern Washington State. The Spent Nuclear Fuel Project (SNF Project) is responsible to DOE for operation of these fuel storage pools and for the 2100 metric tons of SNF materials that they contain. The SNF Project mission includes safe removal and transportation of all SNF from these storage basins to a new storage facility in the 200 East Area. To accomplish this mission, the SNF Project modifies the existing KE Basin and KW Basin facilities and constructs two new facilities: the 100 K Area Cold Vacuum Drying Facility (CVDF), which drains and dries the SNF; and the 200 East Area Canister Storage Building (CSB), which stores the SNF. The purpose of this document is to describe the design basis feed compositions for materials stored or processed by SNF Project facilities and activities. This document is not intended to replace the Hanford Spent Fuel Inventory Baseline (WHC 1994b), but only to supplement it by providing more detail on the chemical and radiological inventories in the fuel (this volume) and sludge. A variety of feed definitions is required to support evaluation of specific facility and process considerations during the development of these new facilities. Six separate feed types have been identified for development of new storage or processing facilities. The approach for using each feed during design evaluations is to calculate the proposed facility flowsheet assuming each feed. The process flowsheet would then provide a basis for material compositions and quantities which are used in follow-on calculations.

    • Comment on “Rethinking first-principles electron transport theories with projection operators: The problems caused by partitioning the basis set” [J. Chem. Phys. 139, 114104 (2013)

      SciTech Connect (OSTI)

      Brandbyge, Mads

      2014-05-07

      In a recent paper Reuter and Harrison [J. Chem. Phys. 139, 114104 (2013)] question the widely used mean-field electron transport theories, which employ nonorthogonal localized basis sets. They claim these can violate an “implicit decoupling assumption,” leading to wrong results for the current, different from what would be obtained by using an orthogonal basis, and dividing surfaces defined in real-space. We argue that this assumption is not required to be fulfilled to get exact results. We show how the current/transmission calculated by the standard Greens function method is independent of whether or not the chosen basis set is nonorthogonal, and that the current for a given basis set is consistent with divisions in real space. The ambiguity known from charge population analysis for nonorthogonal bases does not carry over to calculations of charge flux.

    • Basis for Section 3116 Determination for the Idaho Nuclear Technology and Engineering Center Tank Farm Facility at the Idaho National Laboratory

      Broader source: Energy.gov [DOE]

      This 3116 Basis Document addresses the disposal of stabilized residuals in the TFF, and the TFF tank system, and disposal of the tanks, vaults, and associated piping and ancillary equipment at...

    • Technical basis for radiological release of Grand Junction Office Building 2. Volume 2, dose assessment supporting data

      SciTech Connect (OSTI)

      1997-07-01

      The second volume of the Grand Junction Office Action Program Technical Basis for Radiological Release of Grand Junction Office Building 2 report includes the data quality objectives (DQO), sampling plan, collected data, and analysis used to model future radiation doses to members of the public occupying Building 2 on the U.S. Department of Energy (DOE) Grand Junction Office (GJO) site. This volume was assembled by extracting relevant components of the Grand Junction Projects Office Remedial Action Project Building 2 Public Dose Evaluation (DOE 1996) and inserting recent additional data that was gathered and dose pathway modeling that was performed. The intent of this document is to provide all derived guidance decisions, assumptions, measured data, testing results, and pathway modeling software input and output data that supports the discussion and determinations presented in Volume 1 of this report. For constructive employment of this document, the reader is encouraged to closely follow Volume 1 for proper association with the segment of information being examined.

    • Structural Basis of Ubiquitin Recognition by the Ubiquitin-associated (UBA) Domain of the Ubiquitin Ligase EDD

      SciTech Connect (OSTI)

      Kozlov, G.; Nguyen, L; Lin, T; De Crescenzo, G; Park, M; Gehring, K

      2007-01-01

      EDD (or HYD) is an E3 ubiquitin ligase in the family of HECT (homologous to E6-AP C terminus) ligases. EDD contains an N-terminal ubiquitin-associated (UBA) domain, which is present in a variety of proteins involved in ubiquitin-mediated processes. Here, we use isothermal titration calorimetry (ITC), NMR titrations, and pull-down assays to show that the EDD UBA domain binds ubiquitin. The 1.85{angstrom} crystal structure of the complex with ubiquitin reveals the structural basis of ubiquitin recognition by UBA helices {alpha}1 and {alpha}3. The structure shows a larger number of intermolecular hydrogen bonds than observed in previous UBA/ubiquitin complexes. Two of these involve ordered water molecules. The functional importance of residues at the UBA/ubiquitin interface was confirmed using site-directed mutagenesis. Surface plasmon resonance (SPR) measurements show that the EDD UBA domain does not have a strong preference for polyubiquitin chains over monoubiquitin. This suggests that EDD binds to monoubiquitinated proteins, which is consistent with its involvement in DNA damage repair pathways.

    • Structural Basis for a Switch in Receptor Binding Specificity of Two H5N1 Hemagglutinin Mutants

      DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

      Zhu, Xueyong; Viswanathan, Karthik; Raman, Rahul; Yu, Wenli; Sasisekharan, Ram; Wilson, Ian A.

      2015-11-01

      Avian H5N1 influenza viruses continue to spread in wild birds and domestic poultry with sporadic infection in humans. Receptor binding specificity changes are a prerequisite for H5N1 viruses and other zoonotic viruses to be transmitted among humans. Previous reported hemagglutinin (HA) mutants from ferret-transmissible H5N1 viruses of A/Viet Nam/1203/04 and A/Indonesia/5/05 showed slightly increased, but still very weak, binding to human receptors. From mutagenesis and glycan array studies, we previously identified two H5N1 HA mutants that could more effectively switch receptor specificity to human-like α2-6 linked sialosides with avidity comparable to wild-type H5 HA binding to avian-like α2-3 linked sialosides.more »Here, crystal structures of these two H5 HA mutants free and in complex with human and avian glycan receptor analogues reveal the structural basis for their preferential binding to human receptors. These findings suggest continuous surveillance should be maintained to monitor and assess human-to-human transmission potential of H5N1 viruses.« less

    • Establishing a Cost Basis for Converting the High Flux Isotope Reactor from High Enriched to Low Enriched Uranium Fuel

      SciTech Connect (OSTI)

      Primm, Trent; Guida, Tracey

      2010-02-01

      Under the auspices of the Global Threat Reduction Initiative Reduced Enrichment for Research and Test Reactors Program, the National Nuclear Security Administration /Department of Energy (NNSA/DOE) has, as a goal, to convert research reactors worldwide from weapons grade to non-weapons grade uranium. The High Flux Isotope Reactor (HFIR) at Oak Ridge National Lab (ORNL) is one of the candidates for conversion of fuel from high enriched uranium (HEU) to low enriched uranium (LEU). A well documented business model, including tasks, costs, and schedules was developed to plan the conversion of HFIR. Using Microsoft Project, a detailed outline of the conversion program was established and consists of LEU fuel design activities, a fresh fuel shipping cask, improvements to the HFIR reactor building, and spent fuel operations. Current-value costs total $76 million dollars, include over 100 subtasks, and will take over 10 years to complete. The model and schedule follows the path of the fuel from receipt from fuel fabricator to delivery to spent fuel storage and illustrates the duration, start, and completion dates of each subtask to be completed. Assumptions that form the basis of the cost estimate have significant impact on cost and schedule.

    • Testing model energy spectra of charged particles produced in hadron interactions on the basis of atmospheric muons

      SciTech Connect (OSTI)

      Dedenko, L. G.; Roganova, T. M.; Fedorova, G. F.

      2015-10-15

      An original method for calculating the spectrum of atmospheric muons with the aid of the CORSIKA 7.4 code package and numerical integration is proposed. The first step consists in calculating the energy distribution of muons for various fixed energies of primary-cosmic-ray particles and within several chosen hadron-interaction models included in the CORSIKA 7.4 code package. After that, the spectrum of atmospheric muons is calculated via integrating the resulting distribution densities with the chosen spectrum of primary-cosmic-ray particles. The atmospheric-muon fluxes that were calculated on the basis of the SIBYLL 2.1, QGSJET01, and QGSJET II-04 models exceed the predictions of the wellknown Gaisser approximation of this spectrum by a factor of 1.5 to 1.8 in the range of muon energies between about 10{sup 3} and 10{sup 4} GeV.Under the assumption that, in the region of extremely highmuon energies, a dominant contribution to the muon flux comes from one to two generations of charged π{sup ±} and K{sup ±} mesons, the production rate calculated for these mesons is overestimated by a factor of 1.3 to 1.5. This conclusion is confirmed by the results of the LHCf and TOTEM experiments.

  1. Criteria for calculating the efficiency of deep-pleated HEPA filters with aluminum separators during and after design basis accidents

    SciTech Connect (OSTI)

    Bergman, W.; First, M.W.; Anderson, W.L.; Gilbert, H.; Jacox, J.W.

    1995-02-01

    The authors have reviewed the literature on the performance of high efficiency particulate air (HEPA) filters under normal and abnormal conditions to establish criteria for calculating the efficiency of HEPA filters in a DOE nonreactor nuclear facility during and after a Design Basis Accident (DBA). This study is only applicable to the standard deep-pleated HEPA filter with aluminum separators as specified in ASME N509. The literature review included the performance of new filters and parameters that may cause deterioration in the filter performance such as filter age, radiation, corrosive chemicals, seismic and rough handling, high temperature, moisture, particle clogging, high air flow and pressure pulses. The deterioration of the filter efficiency depends on the exposure parameters; in severe exposure conditions the filter will be structurally damaged and have a residual efficiency of 0%. Despite the many studies on HEPA filter performance under adverse conditions, there are large gaps and limitations in the data that introduce significant error in the estimates of HEPA filter efficiencies under DBA conditions. Because of this limitation, conservative values of filter efficiency were chosen when there was insufficient data.

  2. Local Physics Basis of Confinement Degradation in JET ELMy H-Mode Plasmas and Implications for Tokamak Reactors

    SciTech Connect (OSTI)

    Budny, R.V.; Alper, B.; Borba, D.; Cordey, J.G.; Ernst, D.R.; Gowers, C.

    2001-02-02

    First results of gyrokinetic analysis of JET [Joint European Torus] ELMy [Edge Localized Modes] H-mode [high-confinement modes] plasmas are presented. ELMy H-mode plasmas form the basis of conservative performance predictions for tokamak reactors of the size of ITER [International Thermonuclear Experimental Reactor]. Relatively high performance for long duration has been achieved and the scaling appears to be favorable. It will be necessary to sustain low Z(subscript eff) and high density for high fusion yield. This paper studies the degradation in confinement and increase in the anomalous heat transport observed in two JET plasmas: one with an intense gas puff and the other with a spontaneous transition between Type I to III ELMs at the heating power threshold. Linear gyrokinetic analysis gives the growth rate, gamma(subscript lin) of the fastest growing modes. The flow-shearing rate omega(subscript ExB) and gamma(subscript lin) are large near the top of the pedestal. Their ratio decreases approximately when the confinement degrades and the transport increases. This suggests that tokamak reactors may require intense toroidal or poloidal torque input to maintain sufficiently high |gamma(subscript ExB)|/gamma(subscript lin) near the top of the pedestal for high confinement.

  3. Possible Methods to Estimate Core Location in a Beyond-Design-Basis Accident at a GE BWR with a Mark I Containment Stucture

    SciTech Connect (OSTI)

    Walston, S; Rowland, M; Campbell, K

    2011-07-27

    It is difficult to track to the location of a melted core in a GE BWR with Mark I containment during a beyond-design-basis accident. The Cooper Nuclear Station provided a baseline of normal material distributions and shielding configurations for the GE BWR with Mark I containment. Starting with source terms for a design-basis accident, methods and remote observation points were investigated to allow tracking of a melted core during a beyond-design-basis accident. The design of the GE BWR with Mark-I containment highlights an amazing poverty of expectations regarding a common mode failure of all reactor core cooling systems resulting in a beyond-design-basis accident from the simple loss of electric power. This design is shown in Figure 1. The station blackout accident scenario has been consistently identified as the leading contributor to calculated probabilities for core damage. While NRC-approved models and calculations provide guidance for indirect methods to assess core damage during a beyond-design-basis loss-of-coolant accident (LOCA), there appears to be no established method to track the location of the core directly should the LOCA include a degree of fuel melt. We came to the conclusion that - starting with detailed calculations which estimate the release and movement of gaseous and soluble fission products from the fuel - selected dose readings in specific rooms of the reactor building should allow the location of the core to be verified.

  4. Excitation of helium Rydberg states and doubly excited resonances in strong extreme ultraviolet fields: Full-dimensional quantum dynamics using exponentially tempered Gaussian basis sets

    SciTech Connect (OSTI)

    Kaprlov-?nsk, Petra Ruth; mydke, Jan; Department of Radiation and Chemical Physics, Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21 Prague 8 ; Civi, Svatopluk

    2013-09-14

    Recently optimized exponentially tempered Gaussian basis sets [P. R. Kapralova-Zdanska and J. Smydke, J. Chem. Phys. 138, 024105 (2013)] are employed in quantitative simulations of helium absorption cross-sections and two-photon excitation yields of doubly excited resonances. Linearly polarized half-infinite and Gaussian laser pulses at wavelengths 3858 nm and large intensities up to 100 TW/cm{sup 2} are considered. The emphasis is laid on convergence of the results with respect to the quality of the Gaussian basis sets (typically limited by a number of partial waves, density, and spatial extent of the basis functions) as well as to the quality of the basis set of field-free states (typically limited by the maximum rotational quantum number and maximum excitation of the lower electron). Particular attention is paid to stability of the results with respect to varying complex scaling parameter. Moreover, the study of the dynamics is preceded by a thorough check of helium energies and oscillator strengths as they are obtained with the exponentially tempered Gaussian basis sets, being also compared with yet unpublished emission wavelengths measured in electric discharge experiments.

  5. Analysis of main steam isolation valve leakage in design basis accidents using MELCOR 1.8.6 and RADTRAD.

    SciTech Connect (OSTI)

    Salay, Michael; Kalinich, Donald A.; Gauntt, Randall O.; Radel, Tracy E.

    2008-10-01

    Analyses were performed using MELCOR and RADTRAD to investigate main steam isolation valve (MSIV) leakage behavior under design basis accident (DBA) loss-of-coolant (LOCA) conditions that are presumed to have led to a significant core melt accident. Dose to the control room, site boundary and LPZ are examined using both approaches described in current regulatory guidelines as well as analyses based on best estimate source term and system response. At issue is the current practice of using containment airborne aerosol concentrations as a surrogate for the in-vessel aerosol concentration that exists in the near vicinity of the MSIVs. This study finds current practice using the AST-based containment aerosol concentrations for assessing MSIV leakage is non-conservative and conceptually in error. A methodology is proposed that scales the containment aerosol concentration to the expected vessel concentration in order to preserve the simplified use of the AST in assessing containment performance under assumed DBA conditions. This correction is required during the first two hours of the accident while the gap and early in-vessel source terms are present. It is general practice to assume that at {approx}2hrs, recovery actions to reflood the core will have been successful and that further core damage can be avoided. The analyses performed in this study determine that, after two hours, assuming vessel reflooding has taken place, the containment aerosol concentration can then conservatively be used as the effective source to the leaking MSIV's. Recommendations are provided concerning typical aerosol removal coefficients that can be used in the RADTRAD code to predict source attenuation in the steam lines, and on robust methods of predicting MSIV leakage flows based on measured MSIV leakage performance.

  6. Structural basis of the heterodimerization of the MST and RASSF SARAH domains in the Hippo signalling pathway

    SciTech Connect (OSTI)

    Hwang, Eunha; Cheong, Hae-Kap; Mushtaq, Ameeq Ul; Kim, Hye-Yeon; Yeo, Kwon Joo; Kim, Eunhee; Lee, Woo Cheol; Hwang, Kwang Yeon; Cheong, Chaejoon; Jeon, Young Ho

    2014-07-01

    The heterodimeric structure of the MST1 and RASSF5 SARAH domains is presented. A comparison of homodimeric and heterodimeric interactions provides a structural basis for the preferential association of the SARAH heterodimer. Despite recent progress in research on the Hippo signalling pathway, the structural information available in this area is extremely limited. Intriguingly, the homodimeric and heterodimeric interactions of mammalian sterile 20-like (MST) kinases through the so-called ‘SARAH’ (SAV/RASSF/HPO) domains play a critical role in cellular homeostasis, dictating the fate of the cell regarding cell proliferation or apoptosis. To understand the mechanism of the heterodimerization of SARAH domains, the three-dimensional structures of an MST1–RASSF5 SARAH heterodimer and an MST2 SARAH homodimer were determined by X-ray crystallography and were analysed together with that previously determined for the MST1 SARAH homodimer. While the structure of the MST2 homodimer resembled that of the MST1 homodimer, the MST1–RASSF5 heterodimer showed distinct structural features. Firstly, the six N-terminal residues (Asp432–Lys437), which correspond to the short N-terminal 3{sub 10}-helix h1 kinked from the h2 helix in the MST1 homodimer, were disordered. Furthermore, the MST1 SARAH domain in the MST1–RASSF5 complex showed a longer helical structure (Ser438–Lys480) than that in the MST1 homodimer (Val441–Lys480). Moreover, extensive polar and nonpolar contacts in the MST1–RASSF5 SARAH domain were identified which strengthen the interactions in the heterodimer in comparison to the interactions in the homodimer. Denaturation experiments performed using urea also indicated that the MST–RASSF heterodimers are substantially more stable than the MST homodimers. These findings provide structural insights into the role of the MST1–RASSF5 SARAH domain in apoptosis signalling.

  7. Technical basis for flawed cylinder test specification to assure adequate fracture resistance of ISO high-strength steel cylinder

    SciTech Connect (OSTI)

    Rana, M.D.; Smith, J.H.; Tribolet, R.O.

    1997-11-01

    High-pressure industrial gases (such as oxygen, nitrogen, argon, hydrogen, etc.) are stored and transported in portable cylinders. ISO TC58 SC3 has developed a draft specification 9809 for design and fabrication of high-pressure cylinders with maximum tensile strength limitation of 1,100 N/mm{sup 2}. In order to extend the ISO 9809 rules for higher than 1,100 N/mm{sup 2} strength level cylinders, a working group WG14 was formed in 1989 to develop new rules to assure adequate fracture resistance. In 1994, WG14 recommended a simple, but unique flawed cylinder test method for design qualification of the cylinder and acceptance criteria to assure adequate fracture resistance. WG14 also recommended Charpy-V-notch impact tests to control the required fracture resistance on production cylinders. This paper presents the technical basis that was employed in developing the flawed cylinder test method and acceptance criteria. The specification was developed for seamless steel cylinders having actual strength in the range of 1,100 to 1,400 N/mm{sup 2} and cylindrical section wall thickness in the range of 3 to 10 mm. Flawed cylinder tests were conducted on several hundred cylinders of varying sizes and strength levels. The specification requires to demonstrate LEAK-BEFORE-BREAK performance of the cylinder having flaw length equal to 1.6 (o.d. {times} t{sub design}){sup 0.5} at failure pressure = (t{sub design}/t{sub actual}) x Design Pressure.

  8. Technical basis for flawed cylinder test specification to assure adequate fracture resistance of ISO high strength steel cylinder

    SciTech Connect (OSTI)

    Rana, M.D.; Smith, J.H.; Tribolet, R.O.

    1996-12-01

    High pressure industrial gases (such as oxygen, nitrogen, argon, hydrogen, etc.) are stored and transported in portable cylinders. ISO TC58 SC3 has developed a draft specification 9809 for design and fabrication of high pressure cylinders with maximum tensile strength limitation of 1,100 N/mm{sup 2}. In order to extend the ISO 9809 rules for higher than 1,100 N/mm{sup 2} strength level cylinders, a working group WG14 was formed in 1989 to develop new rules to assure adequate fracture resistance. In 1994, WG14 recommended a simple, but unique flawed cylinder test method for design qualification of the cylinder and acceptance criteria to assure adequate fracture resistance. WG14 also recommended Charpy-V-Notch impact tests to control the required fracture resistance on production cylinders. This paper presents the technical basis that was employed in developing the flawed cylinder test method and acceptance criteria. The specification was developed for seamless steel cylinders having actual strength in the range of 1,100 to 1,400 N/mm{sup 2} and cylindrical section wall thickness in the range of 3mm to 10mm. Flawed cylinder tests were conducted on several hundred cylinders of varying sizes and strength levels. The specification requires to demonstrate LEAK-BEFORE-BREAK performance of the cylinder having flaw length equal to 1.6(O.D. {times} t{sub design}){sup 0.5} at failure pressure = (t{sub design}/t{sub actual}) {times} Design Pressure.

  9. Ab Initio Calculation of Nuclear Magnetic Resonance Chemical Shift Anisotropy Tensors 1. Influence of Basis Set on the Calculation of 31P Chemical Shifts

    SciTech Connect (OSTI)

    Alam, T.M.

    1998-09-01

    The influence of changes in the contracted Gaussian basis set used for ab initio calculations of nuclear magnetic resonance (NMR) phosphorous chemical shift anisotropy (CSA) tensors was investigated. The isotropic chemical shitl and chemical shift anisotropy were found to converge with increasing complexity of the basis set at the Hartree-Fock @IF) level. The addition of d polarization function on the phosphorous nucIei was found to have a major impact of the calculated chemical shi~ but diminished with increasing number of polarization fimctions. At least 2 d polarization fimctions are required for accurate calculations of the isotropic phosphorous chemical shift. The introduction of density fictional theory (DFT) techniques through tie use of hybrid B3LYP methods for the calculation of the phosphorous chemical shift tensor resulted in a poorer estimation of the NMR values, even though DFT techniques result in improved energy and force constant calculations. The convergence of the W parametem with increasing basis set complexity was also observed for the DFT calculations, but produced results with consistent large deviations from experiment. The use of a HF 6-31 l++G(242p) basis set represents a good compromise between accuracy of the simulation and the complexity of the calculation for future ab initio calculations of 31P NMR parameters in larger complexes.

  10. Enterprise Assessments Review of the Delegation of Safety Basis Approval Authority for Hazard Category 1, 2, and 3 Nuclear Facilities … April 2016

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Review of the Delegation of Safety Basis Approval Authority for Hazard Category 1, 2, and 3 Nuclear Facilities April 2016 Office of Nuclear Safety and Environmental Assessments Office of Environment, Safety and Health Assessments Office of Enterprise Assessments U.S. Department of Energy i Table of Contents Acronyms ...................................................................................................................................................... ii Executive Summary

  11. Enterprise Assessments Targeted Assessment of the Waste Treatment and Immobilization Plant High-Level Waste Facility Radioactive Liquid Waste Disposal System Safety Basis Change Package … May 2016

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Assessment of the Waste Treatment and Immobilization Plant High-Level Waste Facility Radioactive Liquid Waste Disposal System Safety Basis Change Package May 2016 Office of Nuclear Safety and Environmental Assessments Office of Environment, Safety and Health Assessments Office of Enterprise Assessments U.S. Department of Energy i Table of Contents Acronyms

  12. Enterprise Assessments Targeted Review of the Safety Basis at the Savannah River Site F-Area Central Laboratory Facility … January 2016

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Review of the Safety Basis at the Savannah River Site F-Area Central Laboratory Facility January 2016 Office of Nuclear Safety and Environmental Assessments Office of Environment, Safety and Health Assessments Office of Enterprise Assessments U.S. Department of Energy i Table of Contents Acronyms ...................................................................................................................................................... ii Executive Summary

  13. Appraisal of the Uranium Processing Facility Safety Basis Preliminary Safety Design Report Process at the Y-12 National Security Complex, May 2013

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Independent Oversight Appraisal of the Uranium Processing Facility Safety Basis Preliminary Safety Design Report Process at the Y-12 National Security Complex May 2011 May 2013 Office of Safety and Emergency Management Evaluations Office of Enforcement and Oversight Office of Health, Safety and Security U. S. Department of Energy Table of Contents 1.0 Purpose

  14. A computational method for solving stochastic Itô–Volterra integral equations based on stochastic operational matrix for generalized hat basis functions

    SciTech Connect (OSTI)

    Heydari, M.H.; Hooshmandasl, M.R.; Maalek Ghaini, F.M.; Cattani, C.

    2014-08-01

    In this paper, a new computational method based on the generalized hat basis functions is proposed for solving stochastic Itô–Volterra integral equations. In this way, a new stochastic operational matrix for generalized hat functions on the finite interval [0,T] is obtained. By using these basis functions and their stochastic operational matrix, such problems can be transformed into linear lower triangular systems of algebraic equations which can be directly solved by forward substitution. Also, the rate of convergence of the proposed method is considered and it has been shown that it is O(1/(n{sup 2}) ). Further, in order to show the accuracy and reliability of the proposed method, the new approach is compared with the block pulse functions method by some examples. The obtained results reveal that the proposed method is more accurate and efficient in comparison with the block pule functions method.

  15. Report to the Secretary of Energy on Beyond Design Basis Event Pilot Evaluations, Results and Recommendations for Improvements to Enhance Nuclear Safety at DOE Nuclear Facilities, January 2013

    Office of Energy Efficiency and Renewable Energy (EERE)

    In the six months after the March 2011 Fukushima Daiichi nuclear power plant accident in Japan, the U.S. Department of Energy (DOE) took several actions to review the safety of its nuclear facilities and identify situations where near-term improvements could be made. These actions and recommendations were addressed in an August 2011 report to the Secretary of Energy, Review of Requirements and Capabilities for Analyzing and Responding to Beyond Design Basis Events.

  16. Enterprise Assessments Operational Awareness Record for the Review of the Technical Planning Basis at the Waste Isolation Pilot Plant (EA-WIPP-TPB-2016-04-05)

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    EA Operational Awareness Record Report Number: EA-WIPP-TPB-2016-04-05 Site: Waste Isolation Pilot Plant Subject: Observation of Emergency Management Technical Planning Dates of Activity: 04/05/16 - 04/21/16 Report Preparer: Kurt Runge Activity Description/Purpose: The U.S. Department of Energy (DOE) Office of Emergency Management Assessments, within the independent Office of Enterprise Assessments (EA) reviewed portions of the technical planning basis for the Waste Isolation Pilot Plant (WIPP)

  17. On the ground state calculation of a many-body system using a self-consistent basis and quasi-Monte Carlo: An application to water hexamer

    SciTech Connect (OSTI)

    Georgescu, Ionu? Mandelshtam, Vladimir A.; Jitomirskaya, Svetlana

    2013-11-28

    Given a quantum many-body system, the Self-Consistent Phonons (SCP) method provides an optimal harmonic approximation by minimizing the free energy. In particular, the SCP estimate for the vibrational ground state (zero temperature) appears to be surprisingly accurate. We explore the possibility of going beyond the SCP approximation by considering the system Hamiltonian evaluated in the harmonic eigenbasis of the SCP Hamiltonian. It appears that the SCP ground state is already uncoupled to all singly- and doubly-excited basis functions. So, in order to improve the SCP result at least triply-excited states must be included, which then reduces the error in the ground state estimate substantially. For a multidimensional system two numerical challenges arise, namely, evaluation of the potential energy matrix elements in the harmonic basis, and handling and diagonalizing the resulting Hamiltonian matrix, whose size grows rapidly with the dimensionality of the system. Using the example of water hexamer we demonstrate that such calculation is feasible, i.e., constructing and diagonalizing the Hamiltonian matrix in a triply-excited SCP basis, without any additional assumptions or approximations. Our results indicate particularly that the ground state energy differences between different isomers (e.g., cage and prism) of water hexamer are already quite accurate within the SCP approximation.

  18. Generalizing the self-healing diffusion Monte Carlo approach to finite temperature: a path for the optimization of low-energy many-body basis expansions

    SciTech Connect (OSTI)

    Kim, Jeongnim; Reboredo, Fernando A

    2014-01-01

    The self-healing diffusion Monte Carlo method for complex functions [F. A. Reboredo J. Chem. Phys. {\\bf 136}, 204101 (2012)] and some ideas of the correlation function Monte Carlo approach [D. M. Ceperley and B. Bernu, J. Chem. Phys. {\\bf 89}, 6316 (1988)] are blended to obtain a method for the calculation of thermodynamic properties of many-body systems at low temperatures. In order to allow the evolution in imaginary time to describe the density matrix, we remove the fixed-node restriction using complex antisymmetric trial wave functions. A statistical method is derived for the calculation of finite temperature properties of many-body systems near the ground state. In the process we also obtain a parallel algorithm that optimizes the many-body basis of a small subspace of the many-body Hilbert space. This small subspace is optimized to have maximum overlap with the one expanded by the lower energy eigenstates of a many-body Hamiltonian. We show in a model system that the Helmholtz free energy is minimized within this subspace as the iteration number increases. We show that the subspace expanded by the small basis systematically converges towards the subspace expanded by the lowest energy eigenstates. Possible applications of this method to calculate the thermodynamic properties of many-body systems near the ground state are discussed. The resulting basis can be also used to accelerate the calculation of the ground or excited states with Quantum Monte Carlo.

  19. TU-F-18A-04: Use of An Image-Based Material-Decomposition Algorithm for Multi-Energy CT to Determine Basis Material Densities

    SciTech Connect (OSTI)

    Li, Z; Leng, S; Yu, L; McCollough, C

    2014-06-15

    Purpose: Published methods for image-based material decomposition with multi-energy CT images have required the assumption of volume conservation or accurate knowledge of the x-ray spectra and detector response. The purpose of this work was to develop an image-based material-decomposition algorithm that can overcome these limitations. Methods: An image-based material decomposition algorithm was developed that requires only mass conservation (rather than volume conservation). With this method, using multi-energy CT measurements made with n=4 energy bins, the mass density of each basis material and of the mixture can be determined without knowledge of the tube spectra and detector response. A digital phantom containing 12 samples of mixtures from water, calcium, iron, and iodine was used in the simulation (Siemens DRASIM). The calibration was performed by using pure materials at each energy bin. The accuracy of the technique was evaluated in noise-free and noisy data under the assumption of an ideal photon-counting detector. Results: Basis material densities can be estimated accurately by either theoretic calculation or calibration with known pure materials. The calibration approach requires no prior information about the spectra and detector response. Regression analysis of theoretical values versus estimated values results in excellent agreement for both noise-free and noisy data. For the calibration approach, the R-square values are 0.9960+/−0.0025 and 0.9476+/−0.0363 for noise-free and noisy data, respectively. Conclusion: From multi-energy CT images with n=4 energy bins, the developed image-based material decomposition method accurately estimated 4 basis material density (3 without k-edge and 1 with in the range of the simulated energy bins) even without any prior information about spectra and detector response. This method is applicable to mixtures of solutions and dissolvable materials, where volume conservation assumptions do not apply. CHM receives

  20. TECHNICAL BASIS FOR DOE STANDARD 3013 EQUIVALENCY SUPPORTING REDUCED TEMPERATURE STABILIZATION OF OXALATE-DERIVED PLUTONIUM DIOXIDE PRODUCED BY THE HB-LINE FACILITY AT SAVANNAH RIVER SITE

    SciTech Connect (OSTI)

    Duffey, J. M.; Livingston, R. R.; Berg, J. M.; Veirs, D. K.

    2013-02-06

    This report documents the technical basis for determining that stabilizing highpurity PuO{sub 2} derived from oxalate precipitation at the SRS HB-Line facility at a minimum of 625 {degree}C for at least four hours in an oxidizing atmosphere is equivalent to stabilizing at a minimum of 950 {degree}C for at least two hours as regards meeting the objectives of stabilization defined by DOE-STD-3013 if the material is handled in a way to prevent excessive absorption of water.

  1. Appendices for the Basis Document

    Broader source: Energy.gov [DOE]

    INTEC Tank Farm Facility Closure Supporting Tables and Photographs - In support of the Tank Farm Facility (TFF) closure, inventory tables were generated for all tanks that have been cleaned up to...

  2. Design Basis Threat Policy (U)

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2005-11-29

    This Order is classified and will not be available on the Directives Portal. For distribution, contact John Fitzgibbons, 301-903-1361, john.fitzgibbons@hq.doe.gov. Cancels: DOE O 470.3

  3. Design Basis Threat Policy (U)

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2004-10-01

    This Order is classified and will not be available on the Directives Portal. For distribution, NA employees should contact Jon Todd, 202-586-2579; all other DOE employees should contact Ron Francis, 301-903-6874. Does not cancel other directives.

  4. Appendices for the Basis Document

    Office of Environmental Management (EM)

    ... concentrations are from the same data set as described in Section D-1.1. D-1.2.3 ... A decision error occurs when the sample data set misleads the decision-maker into the ...

  5. Basis Document for Sludge Stabilization

    SciTech Connect (OSTI)

    WILKINS, N.E.

    2000-04-03

    Vault personnel have begun a program of radiographing plutonium metal cans. Metal that appears to be corroded will be processed through the furnaces in Glovebox HC-21C. The fire suppression system activation temperature will be deleted because it is not maintained by PFP Stabilization.

  6. Technical basis for dose reconstruction

    SciTech Connect (OSTI)

    Anspaugh, L.R.

    1996-01-31

    The purpose of this paper is to consider two general topics: technical considerations of why dose-reconstruction studies should or should not be performed and methods of dose reconstruction. The first topic is of general and growing interest as the number of dose-reconstruction studies increases, and one asks the question whether it is necessary to perform a dose reconstruction for virtually every site at which, for example, the Department of Energy (DOE) has operated a nuclear-related facility. And there is the broader question of how one might logically draw the line at performing or not performing dose-reconstruction (radiological and chemical) studies for virtually every industrial complex in the entire country. The second question is also of general interest. There is no single correct way to perform a dose-reconstruction study, and it is important not to follow blindly a single method to the point that cheaper, faster, more accurate, and more transparent methods might not be developed and applied.

  7. Electronic structure basis for the extraordinary magnetoresistance in WTe2

    SciTech Connect (OSTI)

    Pletikosi?, I.; Ali, Mazhar N.; Fedorov, A. V.; Cava, R. J.; Valla, T.

    2014-11-19

    The electronic structure basis of the extremely large magnetoresistance in layered non-magnetic tungsten ditelluride has been investigated by angle-resolved photoelectron spectroscopy. Hole and electron pockets of approximately the same size were found at the Fermi level, suggesting that carrier compensation should be considered the primary source of the effect. The material exhibits a highly anisotropic, quasi one-dimensional Fermi surface from which the pronounced anisotropy of the magnetoresistance follows. As a result, a change in the Fermi surface with temperature was found and a high-density-of-states band that may take over conduction at higher temperatures and cause the observed turn-on behavior of the magnetoresistance in WTe? was identified.

  8. Crystallographic analysis of human hemoglobin elucidates the structural basis of the potent and dual antisickling activity of pyridyl derivatives of vanillin

    SciTech Connect (OSTI)

    Abdulmalik, Osheiza; Ghatge, Mohini S.; Musayev, Faik N.; Parikh, Apurvasena; Chen, Qiukan; Yang, Jisheng; Nnamani, Ijeoma; Danso-Danquah, Richmond; Eseonu, Dorothy N.; Asakura, Toshio; Abraham, Donald J.; Venitz, Jurgen; Safo, Martin K.

    2011-11-01

    Pyridyl derivatives of vanillin increase the fraction of the more soluble oxygenated sickle hemoglobin and/or directly increase the solubility of deoxygenated sickle hemoglobin. Crystallographic analysis reveals the structural basis of the potent and dual antisickling activity of these derivatives. Vanillin has previously been studied clinically as an antisickling agent to treat sickle-cell disease. In vitro investigations with pyridyl derivatives of vanillin, including INN-312 and INN-298, showed as much as a 90-fold increase in antisickling activity compared with vanillin. The compounds preferentially bind to and modify sickle hemoglobin (Hb S) to increase the affinity of Hb for oxygen. INN-312 also led to a considerable increase in the solubility of deoxygenated Hb S under completely deoxygenated conditions. Crystallographic studies of normal human Hb with INN-312 and INN-298 showed that the compounds form Schiff-base adducts with the N-terminus of the ?-subunits to constrain the liganded (or relaxed-state) Hb conformation relative to the unliganded (or tense-state) Hb conformation. Interestingly, while INN-298 binds and directs its meta-positioned pyridine-methoxy moiety (relative to the aldehyde moiety) further down the central water cavity of the protein, that of INN-312, which is ortho to the aldehyde, extends towards the surface of the protein. These studies suggest that these compounds may act to prevent sickling of SS cells by increasing the fraction of the soluble high-affinity Hb S and/or by stereospecific inhibition of deoxygenated Hb S polymerization.

  9. Neutronics Simulations of 237Np Targets to Support Safety-Basis and 238Pu Production Assessment Efforts at the High Flux Isotope Reactor

    SciTech Connect (OSTI)

    Chandler, David; Ellis, Ronald James

    2015-01-01

    Fueled by two highly enriched uranium-bearing fuel elements surrounded by a large concentric ring of beryllium reflector, the High Flux Isotope Reactor (HFIR) provides one of the highest neutron fluxes in the world and is used to produce unique isotopes like plutonium-238. The National Aeronautics and Space Administration use radioisotope thermoelectric generators powered by 238Pu for deep-space missions. As part of the US Department of Energy s task to reestablish the domestic production of 238Pu, a technology demonstration sub-project has been initiated to establish a new 238Pu supply chain. HFIR safety-basis neutronics calculations are being performed to ensure the target irradiations have no adverse impacts on reactor performance and to calculate data required as input to follow-on thermal-structural, thermal-hydraulic and radionuclide/dose analyses. Plutonium-238 production assessments are being performed to estimate the amount of 238Pu that can be produced in HFIR s permanent beryllium reflector. It is estimated that a total of 0.96 1.12 kg 238Pu (~1.28 1.49 kg PuO2 at 85% 238Pu/Pu purity) could be produced per year in HFIR s permanent beryllium reflector irradiation facilities if they are all utilized.

  10. Molecular Basis for Complement Recognition and Inhibition Determined by Crystallographic Studies of the Staphylococcal Complement Inhibitor (SCIN) Bound to C3c and C3b

    SciTech Connect (OSTI)

    Garcia, Brandon L.; Ramyar, Kasra X.; Tzekou, Apostolia; Ricklin, Daniel; McWhorter, William J.; Lambris, John D.; Geisbrecht, Brian V.

    2010-10-22

    The human complement system plays an essential role in innate and adaptive immunity by marking and eliminating microbial intruders. Activation of complement on foreign surfaces results in proteolytic cleavage of complement component 3 (C3) into the potent opsonin C3b, which triggers a variety of immune responses and participates in a self-amplification loop mediated by a multi-protein assembly known as the C3 convertase. The human pathogen Staphylococcus aureus has evolved a sophisticated and potent complement evasion strategy, which is predicated upon an arsenal of potent inhibitory proteins. One of these, the staphylococcal complement inhibitor (SCIN), acts at the level of the C3 convertase (C3bBb) and impairs downstream complement function by trapping the convertase in a stable but inactive state. Previously, we have shown that SCIN binds C3b directly and competitively inhibits binding of human factor H and, to a lesser degree, that of factor B to C3b. Here, we report the co-crystal structures of SCIN bound to C3b and C3c at 7.5 and 3.5 {angstrom} limiting resolution, respectively, and show that SCIN binds a critical functional area on C3b. Most significantly, the SCIN binding site sterically occludes the binding sites of both factor H and factor B. Our results give insight into SCIN binding to activated derivatives of C3, explain how SCIN can recognize C3b in the absence of other complement components, and provide a structural basis for the competitive C3b-binding properties of SCIN. In the future, this may suggest templates for the design of novel complement inhibitors based upon the SCIN structure.

  11. Comparative genomics of the white-rot fungi, Phanerochaete carnosa and P. chrysosporium, to elucidate the genetic basis of the distinct wood types they colonize

    SciTech Connect (OSTI)

    Suzuki, Hitoshi; MacDonald, Jacqueline; Syed, Khajamohiddin; Salamov, Asaf; Hori, Chiaki; Aerts, Andrea; Henrissat, Bernard; Wiebenga, Ad; vanKuyk, Patricia A.; Barry, Kerrie; Lindquist, Erika; LaButti, Kurt; Lapidus, Alla; Lucas, Susan; Coutinho, Pedro; Gong, Yunchen; Samejima, Masahiro; Mahadevan, Radhakrishnan; Abou-Zaid, Mamdouh; de Vries, Ronald P.; Igarashi, Kiyohiko; Yadav, Jagit S.; Grigoriev, Igor V.; Master, Emma R.

    2012-02-17

    Background Softwood is the predominant form of land plant biomass in the Northern hemisphere, and is among the most recalcitrant biomass resources to bioprocess technologies. The white rot fungus, Phanerochaete carnosa, has been isolated almost exclusively from softwoods, while most other known white-rot species, including Phanerochaete chrysosporium, were mainly isolated from hardwoods. Accordingly, it is anticipated that P. carnosa encodes a distinct set of enzymes and proteins that promote softwood decomposition. To elucidate the genetic basis of softwood bioconversion by a white-rot fungus, the present study reports the P. carnosa genome sequence and its comparative analysis with the previously reported P. chrysosporium genome. Results P. carnosa encodes a complete set of lignocellulose-active enzymes. Comparative genomic analysis revealed that P. carnosa is enriched with genes encoding manganese peroxidase, and that the most divergent glycoside hydrolase families were predicted to encode hemicellulases and glycoprotein degrading enzymes. Most remarkably, P. carnosa possesses one of the largest P450 contingents (266 P450s) among the sequenced and annotated wood-rotting basidiomycetes, nearly double that of P. chrysosporium. Along with metabolic pathway modeling, comparative growth studies on model compounds and chemical analyses of decomposed wood components showed greater tolerance of P. carnosa to various substrates including coniferous heartwood. Conclusions The P. carnosa genome is enriched with genes that encode P450 monooxygenases that can participate in extractives degradation, and manganese peroxidases involved in lignin degradation. The significant expansion of P450s in P. carnosa, along with differences in carbohydrate- and lignin-degrading enzymes, could be correlated to the utilization of heartwood and sapwood preparations from both coniferous and hardwood species.

  12. Volatility basis-set approach simulation of organic aerosol formation in East Asia: implications for anthropogenic-biogenic interaction and controllable amounts

    SciTech Connect (OSTI)

    Matsui, H.; Koike, Makoto; Kondo, Yutaka; Takami, A.; Fast, Jerome D.; Kanaya, Y.; Takigawa, M.

    2014-09-16

    Organic aerosol (OA) simulations using the volatility basis-set approach were made for East Asia and its outflow region. Model simulations were evaluated through comparisons with OA measured by aerosol mass spectrometers in and around Tokyo (at Komaba and Kisai in summer 2003 and 2004) and over the outflow region in East Asia (at Fukue and Hedo in spring 2009). The simulations with aging processes of organic vapors reasonably well reproduced mass concentrations, temporal variations, and formation efficiency of observed OA at all sites. As OA mass was severely underestimated in the simulations without the aging processes, the oxidations of organic vapors are essential for reasonable OA simulations over East Asia. By considering the aging processes, simulated OA concentrations considerably increased from 0.24 to 1.28 g m-3 in the boundary layer over the whole of East Asia. OA formed from the interaction of anthropogenic and biogenic sources was also enhanced by the aging processes. The fraction of controllable OA was estimated to be 87 % of total OA over the whole of East Asia, showing that most of the OA in our simulations formed anthropogenically (controllable). A large portion of biogenic secondary OA (78 % of biogenic secondary OA) formed through the influence of anthropogenic sources. The high fraction of controllable OA in our simulations is likely because anthropogenic emissions are dominant over East Asia and OA formation is enhanced by anthropogenic sources and their aging processes. Both the amounts (from 0.18 to 1.12 g m-3) and the fraction (from 75 % to 87 %) of controllable OA were increased by aging processes of organic vapors over East Asia.

  13. Structural basis for cargo binding and autoinhibition of Bicaudal-D1 by a parallel coiled-coil with homotypic registry

    SciTech Connect (OSTI)

    Terawaki, Shin-ichi; Yoshikane, Asuka; Higuchi, Yoshiki; Wakamatsu, Kaori

    2015-05-01

    Bicaudal-D1 (BICD1) is an α-helical coiled-coil protein mediating the attachment of specific cargo to cytoplasmic dynein. It plays an essential role in minus end-directed intracellular transport along microtubules. The third C-terminal coiled-coil region of BICD1 (BICD1 CC3) has an important role in cargo sorting, including intracellular vesicles associating with the small GTPase Rab6 and the nuclear pore complex Ran binding protein 2 (RanBP2), and inhibiting the association with cytoplasmic dynein by binding to the first N-terminal coiled-coil region (CC1). The crystal structure of BICD1 CC3 revealed a parallel homodimeric coiled-coil with asymmetry and complementary knobs-into-holes interactions, differing from Drosophila BicD CC3. Furthermore, our binding study indicated that BICD1 CC3 possesses a binding surface for two distinct cargos, Rab6 and RanBP2, and that the CC1-binding site overlaps with the Rab6-binding site. These findings suggest a molecular basis for cargo recognition and autoinhibition of BICD proteins during dynein-dependent intracellular retrograde transport. - Highlights: • BICD1 CC3 is a parallel homodimeric coiled-coil with axial asymmetry. • The coiled-coil packing of BICD1 CC3 is adapted to the equivalent heptad position. • BICD1 CC3 has distinct binding sites for two classes of cargo, Rab6 and RanBP2. • The CC1-binding site of BICD1 CC3 overlaps with the Rab6-binding site.

  14. The Effects of Degraded Digital Instrumentation and Control Systems on Human-system Interfaces and Operator Performance: HFE Review Guidance and Technical Basis

    SciTech Connect (OSTI)

    O'Hara, J.M.; W. Gunther, G. Martinez-Guridi

    2010-02-26

    New and advanced reactors will use integrated digital instrumentation and control (I&C) systems to support operators in their monitoring and control functions. Even though digital systems are typically highly reliable, their potential for degradation or failure could significantly affect operator performance and, consequently, impact plant safety. The U.S. Nuclear Regulatory Commission (NRC) supported this research project to investigate the effects of degraded I&C systems on human performance and plant operations. The objective was to develop human factors engineering (HFE) review guidance addressing the detection and management of degraded digital I&C conditions by plant operators. We reviewed pertinent standards and guidelines, empirical studies, and plant operating experience. In addition, we conducted an evaluation of the potential effects of selected failure modes of the digital feedwater system on human-system interfaces (HSIs) and operator performance. The results indicated that I&C degradations are prevalent in plants employing digital systems and the overall effects on plant behavior can be significant, such as causing a reactor trip or causing equipment to operate unexpectedly. I&C degradations can impact the HSIs used by operators to monitor and control the plant. For example, sensor degradations can make displays difficult to interpret and can sometimes mislead operators by making it appear that a process disturbance has occurred. We used the information obtained as the technical basis upon which to develop HFE review guidance. The guidance addresses the treatment of degraded I&C conditions as part of the design process and the HSI features and functions that support operators to monitor I&C performance and manage I&C degradations when they occur. In addition, we identified topics for future research.

  15. Information basis for developing comprehensive waste management system-US-Japan joint nuclear energy action plan waste management working group phase I report.

    SciTech Connect (OSTI)

    Nutt, M.; Nuclear Engineering Division

    2010-05-25

    The activity of Phase I of the Waste Management Working Group under the United States - Japan Joint Nuclear Energy Action Plan started in 2007. The US-Japan JNEAP is a bilateral collaborative framework to support the global implementation of safe, secure, and sustainable, nuclear fuel cycles (referred to in this document as fuel cycles). The Waste Management Working Group was established by strong interest of both parties, which arise from the recognition that development and optimization of waste management and disposal system(s) are central issues of the present and future nuclear fuel cycles. This report summarizes the activity of the Waste Management Working Group that focused on consolidation of the existing technical basis between the U.S. and Japan and the joint development of a plan for future collaborative activities. Firstly, the political/regulatory frameworks related to nuclear fuel cycles in both countries were reviewed. The various advanced fuel cycle scenarios that have been considered in both countries were then surveyed and summarized. The working group established the working reference scenario for the future cooperative activity that corresponds to a fuel cycle scenario being considered both in Japan and the U.S. This working scenario involves transitioning from a once-through fuel cycle utilizing light water reactors to a one-pass uranium-plutonium fuel recycle in light water reactors to a combination of light water reactors and fast reactors with plutonium, uranium, and minor actinide recycle, ultimately concluding with multiple recycle passes primarily using fast reactors. Considering the scenario, current and future expected waste streams, treatment and inventory were discussed, and the relevant information was summarized. Second, the waste management/disposal system optimization was discussed. Repository system concepts were reviewed, repository design concepts for the various classifications of nuclear waste were summarized, and the factors

  16. TECHNICAL BASIS FOR DOE STANDARD 3013 EQUIVALENCY SUPPORTING REDUCED TEMPERATURE STABILIZATION OF OXALATE-DERIVED PLUTONIUM OXIDE PRODUCED BY THE HB-LINE FACILITY AT SAVANNAH RIVER SITE

    SciTech Connect (OSTI)

    Duffey, J.; Livingston, R.; Berg, J.; Veirs, D.

    2012-07-02

    The HB-Line (HBL) facility at the Savannah River Site (SRS) is designed to produce high-purity plutonium dioxide (PuO{sub 2}) which is suitable for future use in production of Mixed Oxide (MOX) fuel. The MOX Fuel Fabrication Facility (MFFF) requires PuO{sub 2} feed to be packaged per the U.S. Department of Energy (DOE) Standard 3013 (DOE-STD-3013) to comply with the facility's safety basis. The stabilization conditions imposed by DOE-STD-3013 for PuO{sub 2} (i.e., 950 C for 2 hours) preclude use of the HBL PuO{sub 2} in direct fuel fabrication and reduce the value of the HBL product as MFFF feedstock. Consequently, HBL initiated a technical evaluation to define acceptable operating conditions for production of high-purity PuO{sub 2} that fulfills the DOE-STD-3013 criteria for safe storage. The purpose of this document is to demonstrate that within the defined operating conditions, the HBL process will be equivalent for meeting the requirements of the DOE-STD-3013 stabilization process for plutonium-bearing materials from the DOE complex. The proposed 3013 equivalency reduces the prescribed stabilization temperature for high-purity PuO{sub 2} from oxalate precipitation processes from 950 C to 640 C and places a limit of 60% on the relative humidity (RH) at the lowest material temperature. The equivalency is limited to material produced using the HBL established flow sheet, for example, nitric acid anion exchange and Pu(IV) direct strike oxalate precipitation with stabilization at a minimum temperature of 640 C for four hours (h). The product purity must meet the MFFF acceptance criteria of 23,600 {micro}g/g Pu (i.e., 2.1 wt %) total impurities and chloride content less than 250 {micro}g/g of Pu. All other stabilization and packaging criteria identified by DOE-STD-3013-2012 or earlier revisions of the standard apply. Based on the evaluation of test data discussed in this document, the expert judgment of the authors supports packaging the HBL product under a 3013

  17. Modular HTGR Safety Basis and Approach

    SciTech Connect (OSTI)

    Thomas Hicks

    2011-08-01

    The Next Generation Nuclear Plant (NGNP) will be a licensed commercial high temperature gas-cooled reactor (HTGR) capable of producing electricity and/or high temperature process heat for industrial markets supporting a range of end-user applications. The NGNP Project has adopted the 10 CFR 52 Combined License (COL) process, as recommended in the NGNP Licensing Strategy - A Report to Congress, dated August 2008, as the foundation for the NGNP licensing strategy [DOE/NRC 2008]. Nuclear Regulatory Commission (NRC) licensing of the NGNP plant utilizing this process will demonstrate the efficacy for licensing future HTGRs for commercial industrial applications. This information paper is one in a series of submittals that address key generic issues of the priority licensing topics as part of the process for establishing HTGR regulatory requirements. This information paper provides a summary level introduction to HTGR history, public safety objectives, inherent and passive safety features, radionuclide release barriers, functional safety approach, and risk-informed safety approach. The information in this paper is intended to further the understanding of the modular HTGR safety approach with the NRC staff and public stakeholders. The NGNP project does not expect to receive comments on this information paper because other white papers are addressing key generic issues of the priority licensing topics in greater detail.

  18. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it...

  19. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit (CTA1) triggers a series of events culminating in the massive efflux of...

  20. Setting clear expectations for safety basis development

    SciTech Connect (OSTI)

    MORENO, M.R.

    2003-05-03

    DOE-RL has set clear expectations for a cost-effective approach for achieving compliance with the Nuclear Safety Management requirements (10 CFR 830, Nuclear Safety Rule) which will ensure long-term benefit to Hanford. To facilitate implementation of these expectations, tools were developed to streamline and standardize safety analysis and safety document development resulting in a shorter and more predictable DOE approval cycle. A Hanford Safety Analysis and Risk Assessment Handbook (SARAH) was issued to standardized methodologies for development of safety analyses. A Microsoft Excel spreadsheet (RADIDOSE) was issued for the evaluation of radiological consequences for accident scenarios often postulated for Hanford. A standard Site Documented Safety Analysis (DSA) detailing the safety management programs was issued for use as a means of compliance with a majority of 3009 Standard chapters. An in-process review was developed between DOE and the Contractor to facilitate DOE approval and provide early course correction. As a result of setting expectations and providing safety analysis tools, the four Hanford Site waste management nuclear facilities were able to integrate into one Master Waste Management Documented Safety Analysis (WM-DSA).

  1. Complex basis functions revisited: Implementation with applications...

    Office of Scientific and Technical Information (OSTI)

    Additional Journal Information: Journal Volume: 142; Journal Issue: 5; Journal ID: ISSN 0021-9606 Publisher: American Institute of Physics Sponsoring Org: USDOE Country of ...

  2. EEG's Preliminary Comments on Basis of Characterization

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... Radioassay The reason for removing NDA requirements from the QAPP and placing them in the W ACC Revision 6 is not clear. Please explain. For the four recommendations under Section ...

  3. Nuclear Safety Basis Training Program Manager

    Broader source: Energy.gov [DOE]

    **This is an Excepted Service position. This appointment will not confer Competitive Service career-conditional or career tenure status. This means that if you are selected, you would have to...

  4. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it...

  5. Structural Basis for Activation of Cholera Toxin

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes...

  6. Physics basis for MFTF-B

    SciTech Connect (OSTI)

    Baldwin, D.E.; Logan, B.G.; Simonen, T.C.

    1980-01-16

    The physics topics included here are the following: (1) adiabaticity, (2) MHD stability, (3) radial transport, (4) barrier physics, (5) loss-cone instability, (6) effect of gas recycling and secondary electron emission, (7) Monte Carlo simulations of tandem mirror physics, (8) charge-exchange pumping of thermal barriers, (9) vacuum pumping in the A-cell, and (10) microstability in the central cell. (MOW)

  7. Results from Beyond Design Basis Event Pilots

    Broader source: Energy.gov [DOE]

    Presenter: Mike Hillman, Program Manager, Office of Health, Safety and Security, US Department of Energy

  8. Structural basis for biomolecular recognition in overlapping...

    Office of Scientific and Technical Information (OSTI)

    binding sites in a diiron enzyme system Citation Details In-Document Search ... Sponsoring Org: NSF Country of Publication: United States Language: ENGLISH Word Cloud ...

  9. Readiness to proceed: Characterization planning basis

    SciTech Connect (OSTI)

    Adams, M.R.

    1998-01-19

    This report summarizes characterization requirements, data availability, and data acquisition plans in support of the Phase 1 Waste Feed Readiness to Proceed Mid-Level Logic. It summarizes characterization requirements for the following program planning documents: Waste Feed Readiness Mid-Level Logic and Decomposition (in development); Master blue print (not available); Tank Waste Remediation System (TWRS) Operations and Utilization Plan and Privatization Contract; Enabling assumptions (not available); Privatization low-activity waste (LAW) Data Quality Objective (DQO); Privatization high-level waste (HLW) DQO (draft); Problem-specific DQOs (in development); Interface control documents (draft). Section 2.0 defines the primary objectives for this report, Section 3.0 discusses the scope and assumptions, and Section 4.0 identifies general characterization needs and analyte-specific characterization needs or potential needs included in program documents and charts. Section 4.0 also shows the analyses that have been conducted, and the archive samples that are available for additional analyses. Section 5.0 discusses current plans for obtaining additional samples and analyses to meet readiness-to-proceed requirements. Section 6.0 summarizes sampling needs based on preliminary requirements and discusses other potential characterization needs. Many requirements documents are preliminary. In many cases, problem-specific DQOs have not been drafted, and only general assumptions about the document contents could be obtained from the authors. As a result, the readiness-to-proceed characterization requirements provided in this document are evolving and may change.

  10. Events Beyond Design Safety Basis Analysis

    Broader source: Energy.gov [DOE]

    This Safety Alert provides information on a safety concern related to the identification and mitigation of events that may fall outside those analyzed in the documented safety analysis. [Safety Bulletin 2011-01

  11. Basis for OUO | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE O 471.3 Admin Chg 1, Identifying and Protecting Official Use Only Information. DOE M 471.3-1 Admin Chg 1, Manual for Identifying and Protecting Official Use Only Information. ...

  12. FB Line Basis for Interim Operation

    SciTech Connect (OSTI)

    Shedrow, B.

    1998-10-19

    The safety analysis of the FB-Line Facility indicates that the operation of FB-Line to support the current mission does not present undue risk to the facility and co-located workers, general public, or the environment.

  13. Lawrence Livermore National Laborotory Safety Basis Assessment...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Each action item is verified to be complete, and LSO is notified in writing when the plan ... with LSO Work Instruction (WI) 226.1.1, Writing and Managing Contractor Assessments, ...

  14. Morphological basis of tolerance to ozone

    SciTech Connect (OSTI)

    Evans, M.J.; Dekker, N.P.; Cabral-Anderson, L.J.; Shami, S.G.

    1985-06-01

    The purpose of this research was to study Type 1 epithelial cells in the ozone (O/sub 3/)-tolerant lung epithelium. Rats were made tolerant by exposure to 0.5 ppm O/sub 3/ for 2 days and allowed to recover in air. Reexposure to a lethal concentration of O/sub 3/ (6 ppm) at 3, 7, and 15 days of recovery revealed that tolerance was present at 3 days but almost absent at 7 and 15 days of recovery. Using Type 2 cell proliferation as a means of quantitating Type 1 cell injury, it was observed that when the preexposed rats were reexposed to 0.5 ppm at 3, 7, and 15 days, very little Type 1 cell injury occurred at 3 days. However, at 7 and 15 days the amount of Type 1 cell injury was the same as that associated with the original exposure. To determine whether there was any change in the alveolar epithelial cell populations between the periods of tolerance (3 days) and its decline (7 and 15 days), the percentage of tritiated thymidine (( /sup 3/H)TdR-labeled Type 1 and 2 cells at these times were determined. There was a significant decrease in (/sup 3/H)TdR-labeled Type 1 and 2 cells between the third and fifteenth days of recovery as excess cells were sloughed off and the tissue returned to normal. Using electron microscopic morphometry, Type 1 and 2 cells were then studied during the decline of tolerance. No change was found in the morphology of Type 2 cells; however, the morphology of Type 1 cells revealed a 58% decrease in surface area and a 25% increase in the arithmetic mean thickness when tolerance was present at 3 days. As tolerance declined (7 and 15 days), Type 1 cell morphology returned to normal. It was concluded that tolerance exists when the surface area of a cell exposed to a particular concentration of ozone is small enough so that the existing antioxidant mechanism contained within that cell volume can protect it from damage.

  15. Structural Basis for the Promiscuous Biosynthetic Prenylation...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Torrey Pines Road, La Jolla, California 92037, USA 2Laboratory of Cell Biotechnology, Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo ...

  16. Basis adaptation in homogeneous chaos spaces

    SciTech Connect (OSTI)

    Tipireddy, Ramakrishna; Ghanem, Roger

    2014-02-15

    We present a new method for the characterization of subspaces associated with low-dimensional quantities of interest (QoI). The probability density function of these QoI is found to be concentrated around one-dimensional subspaces for which we develop projection operators. Our approach builds on the properties of Gaussian Hilbert spaces and associated tensor product spaces.

  17. Technical Basis for Assessing Uranium Bioremediation Performance

    SciTech Connect (OSTI)

    PE Long; SB Yabusaki; PD Meyer; CJ Murray; AL N’Guessan

    2008-04-01

    In situ bioremediation of uranium holds significant promise for effective stabilization of U(VI) from groundwater at reduced cost compared to conventional pump and treat. This promise is unlikely to be realized unless researchers and practitioners successfully predict and demonstrate the long-term effectiveness of uranium bioremediation protocols. Field research to date has focused on both proof of principle and a mechanistic level of understanding. Current practice typically involves an engineering approach using proprietary amendments that focuses mainly on monitoring U(VI) concentration for a limited time period. Given the complexity of uranium biogeochemistry and uranium secondary minerals, and the lack of documented case studies, a systematic monitoring approach using multiple performance indicators is needed. This document provides an overview of uranium bioremediation, summarizes design considerations, and identifies and prioritizes field performance indicators for the application of uranium bioremediation. The performance indicators provided as part of this document are based on current biogeochemical understanding of uranium and will enable practitioners to monitor the performance of their system and make a strong case to clients, regulators, and the public that the future performance of the system can be assured and changes in performance addressed as needed. The performance indicators established by this document and the information gained by using these indicators do add to the cost of uranium bioremediation. However, they are vital to the long-term success of the application of uranium bioremediation and provide a significant assurance that regulatory goals will be met. The document also emphasizes the need for systematic development of key information from bench scale tests and pilot scales tests prior to full-scale implementation.

  18. Temporal variability of the trade wind inversion: Measured with a boundary layer vertical profiler. Master's thesis

    SciTech Connect (OSTI)

    Grindinger, C.M.

    1992-05-01

    This study uses Hawaiian Rainband Project (HaRP) data, from the summer of 1991, to show a boundary layer wind profiler can be used to measure the trade wind inversion. An algorithm has been developed for the profiler that objectively measures the depth of the moist oceanic boundary layer. The Hilo inversion, measured by radiosonde, is highly correlated with the moist oceanic boundary layer measured by the profiler at Paradise Park. The inversion height on windward Hawaii is typically 2253 + or - 514 m. The inversion height varies not only on a daily basis, but on less than an hourly basis. It has a diurnal, as well as a three to four day cycle. There appears to be no consistent relationship between inversion height and precipitation. Currently, this profiler is capable of making high frequency (12 minute) measurements of the inversion base variation, as well as other features.

  19. Accurate ab initio-based adiabatic global potential energy surface for the 2{sup 2}A″ state of NH{sub 2} by extrapolation to the complete basis set limit

    SciTech Connect (OSTI)

    Li, Y. Q.; Ma, F. C.; Sun, M. T.

    2013-10-21

    A full three-dimensional global potential energy surface is reported first time for the title system, which is important for the photodissociation processes. It is obtained using double many-body expansion theory and an extensive set of accurate ab initio energies extrapolated to the complete basis set limit. Such a work can be recommended for dynamics studies of the N({sup 2}D) + H{sub 2} reaction, a reliable theoretical treatment of the photodissociation dynamics and as building blocks for constructing the double many-body expansion potential energy surface of larger nitrogen/hydrogen containing systems. In turn, a preliminary theoretical study of the reaction N({sup 2}D)+H{sub 2}(X{sup 1}Σ{sub g}{sup +})(ν=0,j=0)→NH(a{sup 1}Δ)+H({sup 2}S) has been carried out with the method of quasi-classical trajectory on the new potential energy surface. Integral cross sections and thermal rate constants have been calculated, providing perhaps the most reliable estimate of the integral cross sections and the rate constants known thus far for such a reaction.

  20. NEXT GENERATION NUCLEAR PLANT LICENSING BASIS EVENT SELECTION WHITE PAPER

    SciTech Connect (OSTI)

    Mark Holbrook

    2010-09-01

    The Next Generation Nuclear Plant (NGNP) will be a licensed commercial high temperature gas-cooled reactor (HTGR) plant capable of producing the electricity and high temperature process heat for industrial markets supporting a range of end-user applications. The NGNP Project has adopted the 10 CFR 52 Combined License (COL) application process, as recommended in the Report to Congress, dated August 2008, as the foundation for the NGNP licensing strategy. NRC licensing of the NGNP plant utilizing this process will demonstrate the efficacy of licensing future HTGRs for commercial industrial applications. This white paper is one in a series of submittals that will address key generic issues of the COL priority licensing topics as part of the process for establishing HTGR regulatory requirements.

  1. F tank draft basis determination press release 092910 _2_....

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FOR IMMEDIATE RELEASE Jim Giusti, DOE-SRS, (803)952-7697 Friday, October 01, 2010 james-r.giusti@srs.gov DOE Seeks Comment on Waste Determination for F-Tank Farm The Department of Energy (DOE) Savannah River Operations Office (SR) is seeking public comment on a draft waste determination for wastes from reprocessing of spent nuclear fuel from F-Tank Farm (FTF) at the Savannah River Site. Secretary of Energy, in consultation with the U.S. Nuclear Regulatory Commission, has authority under Section

  2. Sculpting bespoke mountains: Determining free energies with basis...

    Office of Scientific and Technical Information (OSTI)

    ; McGovern, Michael 2 ; de Pablo, Juan J. 3 + Show Author Affiliations Department of Chemical and Biomolecular Engineering, University of Notre Dame du Lac, Notre Dame,...

  3. Structural basis of AMPK regulation by adenine nucleotides and glycogen

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Li, Xiaodan; Wang, Lili; Zhou, X. Edward; Ke, Jiyuan; de Waal, Parker W.; Gu, Xin; Tan, M. H. Eileen; Wang, Dongye; Wu, Donghai; Xu, H. Eric; et al

    2014-11-21

    AMP-activated protein kinase (AMPK) is a central cellular energy sensor and regulator of energy homeostasis, and a promising drug target for the treatment of diabetes, obesity, and cancer. Here we present low-resolution crystal structures of the human α1β2γ1 holo-AMPK complex bound to its allosteric modulators AMP and the glycogen-mimic cyclodextrin, both in the phosphorylated (4.05 Å) and non-phosphorylated (4.60 Å) state. In addition, we have solved a 2.95 Å structure of the human kinase domain (KD) bound to the adjacent autoinhibitory domain (AID) and have performed extensive biochemical and mutational studies. Altogether, these studies illustrate an underlying mechanism of allostericmore » AMPK modulation by AMP and glycogen, whose binding changes the equilibria between alternate AID (AMP) and carbohydrate-binding module (glycogen) interactions.« less

  4. A BASIS FOR MODIFYING THE TANK 12 COMPOSITE SAMPLING DESIGN

    SciTech Connect (OSTI)

    Shine, G.

    2014-11-25

    The SRR sampling campaign to obtain residual solids material from the Savannah River Site (SRS) Tank Farm Tank 12 primary vessel resulted in obtaining appreciable material in all 6 planned source samples from the mound strata but only in 5 of the 6 planned source samples from the floor stratum. Consequently, the design of the compositing scheme presented in the Tank 12 Sampling and Analysis Plan, Pavletich (2014a), must be revised. Analytical Development of SRNL statistically evaluated the sampling uncertainty associated with using various compositing arrays and splitting one or more samples for compositing. The variance of the simple mean of composite sample concentrations is a reasonable standard to investigate the impact of the following sampling options. Composite Sample Design Option (a). Assign only 1 source sample from the floor stratum and 1 source sample from each of the mound strata to each of the composite samples. Each source sample contributes material to only 1 composite sample. Two source samples from the floor stratum would not be used. Composite Sample Design Option (b). Assign 2 source samples from the floor stratum and 1 source sample from each of the mound strata to each composite sample. This infers that one source sample from the floor must be used twice, with 2 composite samples sharing material from this particular source sample. All five source samples from the floor would be used. Composite Sample Design Option (c). Assign 3 source samples from the floor stratum and 1 source sample from each of the mound strata to each composite sample. This infers that several of the source samples from the floor stratum must be assigned to more than one composite sample. All 5 source samples from the floor would be used. Using fewer than 12 source samples will increase the sampling variability over that of the Basic Composite Sample Design, Pavletich (2013). Considering the impact to the variance of the simple mean of the composite sample concentrations, the recommendation is to construct each sample composite using four or five source samples. Although the variance using 5 source samples per composite sample (Composite Sample Design Option (c)) was slightly less than the variance using 4 source samples per composite sample (Composite Sample Design Option (b)), there is no practical difference between those variances. This does not consider that the measurement error variance, which is the same for all composite sample design options considered in this report, will further dilute any differences. Composite Sample Design Option (a) had the largest variance for the mean concentration in the three composite samples and should be avoided. These results are consistent with Pavletich (2014b) which utilizes a low elevation and a high elevation mound source sample and two floor source samples for each composite sample. Utilizing the four source samples per composite design, Pavletich (2014b) utilizes aliquots of Floor Sample 4 for two composite samples.

  5. Atomic substitution reveals the structural basis for substrate...

    Office of Scientific and Technical Information (OSTI)

    by an adenine DNA glycosylase, Bacillus stearothermophilus MutY, have previously ... Language: ENGLISH Subject: 08 HYDROGEN; ADENINES; ATOMS; BACILLUS; BONDING; CLEAVAGE; DNA; ...

  6. Enterprise Assessments Review of the Delegation of Safety Basis...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    approval authority (SBAA) within the DOE Offices of Science (SC), Nuclear Energy (NE), and Environmental Management (EM), and the National Nuclear Security Administration (NNSA). ...

  7. Breckinridge Project, initial effort. Report II. Breckinridge Project design basis

    SciTech Connect (OSTI)

    1982-01-01

    The Breckinridge Project is a pioneer endeavor involving the engineering, construction, and operation of a commercial facility that will convert 23,000 tons per day of run-of-mine, high-sulfur coal into 50,000 barrels per day of liquid hydrocarbons equivalent to those produced from crude oil. The Initial Effort, now complete, was executed under Cooperative Agreement No. DE-FC05-80OR20717 between the Department of Energy and the Participants, Ashland Synthetic Fuels, Inc., and Airco Energy Company, Inc. The Initial Effort produced a preliminary design, capital estimate, and economic analysis of the commercial plant, as well as a plan for the design, construction, and operation of that plant. The extensive and rigorous attention given to environmental, socioeconomic, safety, and health considerations is indicative of the high priority these issues will continue to receive throughout the life of the project. The Breckinridge Energy Company, a partnership of several major corporations, is being formed to finance, own, and manage the Breckinridge Project. Report II is intended for the reader who is primarily interested in less detailed discussion of the coal liquefaction process and Breckinridge facility than presented in the eleven volumes of Reports IV and V. The overview section describes the project goals and briefly introduces the coal liquefaction process. The report continues with a discussion of the history of the project and the H-COAL process from its concept to the proposed commercialization technology. The report describes the site, the Breckinridge Facility, and concludes with a summary of the eleven reports that contain the deliverable documentation of the Initial Effort or Development Phase of the project.

  8. Structural Basis for Metallic-Like Conductivity in Microbial Nanowires

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Malvankar, Nikhil S.; Vargas, Madeline; Nevin, Kelly; Tremblay, Pier-Luc; Evans-Lutterodt, Kenneth; Nykypanchuk, Dmytro; Martz, Eric; Tuominen, Mark T.; Lovley, Derek R.

    2015-03-03

    Direct measurement of multiple physical properties of Geobacter sulfurreducens pili have demonstrated that they possess metallic-like conductivity, but several studies have suggested that metallic-like conductivity is unlikely based on the structures of the G. sulfurreducens pilus predicted from homology models. In order to further evaluate this discrepancy, pili were examined with synchrotron X-ray microdiffraction and rocking-curve X-ray diffraction. Both techniques revealed a periodic 3.2-Å spacing in conductive, wild-type G. sulfurreducens pili that was missing in the nonconductive pili of strain Aro5, which lack key aromatic acids required for conductivity. The intensity of the 3.2-Å peak increased 100-fold when the pHmore » was shifted from 10.5 to 2, corresponding with a previously reported 100-fold increase in pilus conductivity with this pH change. These results suggest a clear structure-function correlation for metallic-like conductivity that can be attributed to overlapping π-orbitals of aromatic amino acids. A homology model of the G. sulfurreducens pilus was constructed with a Pseudomonas aeruginosa pilus model as a template as an alternative to previous models, which were based on a Neisseria gonorrhoeae pilus structure. This alternative model predicted that aromatic amino acids in G. sulfurreducens pili are packed within 3 to 4 Å, consistent with the experimental results. Thus, the predictions of homology modeling are highly sensitive to assumptions inherent in the model construction. Finally, the experimental results reported here further support the concept that the pili of G. sulfurreducens represent a novel class of electronically functional proteins in which aromatic amino acids promote long-distance electron transport.« less

  9. The structural basis of RNA-catalyzed RNA polymerization

    SciTech Connect (OSTI)

    Shechner, David M.; Bartel, David P.

    2011-09-08

    Early life presumably required polymerase ribozymes capable of replicating RNA. Known polymerase ribozymes best approximating such replicases use as their catalytic engine an RNA-ligase ribozyme originally selected from random RNA sequences. Here we report 3.15-{angstrom} crystal structures of this ligase trapped in catalytically viable preligation states, with the 3'-hydroxyl nucleophile positioned for in-line attack on the 5'-triphosphate. Guided by metal- and solvent-mediated interactions, the 5'-triphosphate hooks into the major groove of the adjoining RNA duplex in an unanticipated conformation. Two phosphates and the nucleophile jointly coordinate an active-site metal ion. Atomic mutagenesis experiments demonstrate that active-site nucleobase and hydroxyl groups also participate directly in catalysis, collectively playing a role that in proteinaceous polymerases is performed by a second metal ion. Thus artificial ribozymes can use complex catalytic strategies that differ markedly from those of analogous biological enzymes.

  10. Basis for Section 3116 Determination for the Idaho Nuclear Technology...

    Office of Environmental Management (EM)

    ... Spent nuclear fuel reprocessing was curtailed in 1992, and the first-cycle extraction process wastes stored in the TFF were removed and solidified (by calcination) by February 1998 ...

  11. Perspective on Beyond Design Basis Event Analysis and Response

    Broader source: Energy.gov [DOE]

    Presenter: John Schwenker, Nuclear Safety Manager for Liquid Operations, Savannah River Remediation, LLC, Savannah River Site

  12. Homogeneous charge engines -- Basis of cyclic variations. Final report

    SciTech Connect (OSTI)

    Whitelaw, J.H.

    1997-06-30

    The objectives of the Grant required investigations of cyclic variations in a homogeneous-charge engine initially with gas combustion and the air from ranging from near quiescent to the incorporation of swirl and tumble by valve inserts. Later experiments were performed with unleaded gasoline. The measurements included local velocity and cylinder pressure through the four strokes of a single-cylinder engine, under motored and firing conditions and with examination of the flame kernel growth by combinations of photography and flame-ionization gauges. In all cases, the measurements of in-cylinder characteristics were linked to performance as measured in terms of speed and its variability, load and emissions. The experiments progressed to consider deviations from homogeneous charge and included consideration of stratified charge with local injection of a rich mixture in the vicinity of the spark gap so as to establish a flame kernel which would propagate securely into an overall weak mixture.

  13. Initial technical basis for late washing filter cleaning

    SciTech Connect (OSTI)

    Morrissey, M.F.; Dworjanyn, L.O.

    1992-07-23

    Bench scale filter cleaning tests at the Savannah River Technology Center have shown that cross-flow filter elements can be cleaned between late wash filtration runs and restored to original clean water flux conditions. The most effective cleaning technique was high flow axial recirculation, followed by flushing with caustic solution. Simple flushing with oxalic acid and caustic is less effective and is not recommended because of adverse experience in ITP filter cleaning and uncertainty in the.nature of radiolysis by-product contaminants.

  14. Basis for the US Modern Grid Strategy - A Changing World

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Importance of Smart Grid to Our Nation's Energy Future Steve Pullins President, Horizon Energy Group Steve Bossart Senior Manager, National Energy Technology Laboratory Today's Electricity Grid The U.S. electricity grid is an aging infrastructure based largely on designs of the 1950s before the era of the microprocessor and it was mostly constructed in the 1960s and 1970s. Since the 1970s, the U.S. grid has been expanded as needed to meet load growth using the same vintage technologies. The U.S.

  15. Basis for radiation protection of the nuclear worker

    SciTech Connect (OSTI)

    Guevara, F.A.

    1982-01-01

    A description is given of the standards for protection of persons who work in areas that have a potential for radiation exposure. A review is given of the units of radiation exposure and dose equivalent and of the value of the maximum permissible dose limits for occupational exposure. Federal Regulations and Regulatory Guides for radiation protection are discussed. Average occupational equivalent doses experienced in several operations typical of the United States Nuclear Industry are presented and shown to be significantly lower than the maximum permissible. The concept of maintaining radiation doses to As-Low-As-Reasonably-Achievable is discussed and the practice of imposing engineering and administrative controls to provide effective radiation protection for the nuclear worker is described.

  16. Structural Basis of Wnt Signaling Inhibition by Dickkopf Binding...

    Office of Scientific and Technical Information (OSTI)

    Additional Journal Information: Journal Volume: 21; Journal Issue: 5; Journal ID: ISSN 1534-5807 Publisher: Elsevier Sponsoring Org: USDOE Country of Publication: Country unknown...

  17. Structural Basis for Specificity and Flexibility in a Plant 4...

    Office of Scientific and Technical Information (OSTI)

    Resource Relation: Journal Name: Structure; Journal Volume: 23; Journal Issue: (11) ; 11, 2015 Publisher: Elsevier Research Org: Advanced Photon Source (APS), Argonne National ...

  18. Basis for the US Modern Grid Strategy - A Changing World

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ... powered from electricity produced from coal and nuclear plants and renewable ... Grid considering benefits to utilities, consumers, and society; (3) assist state ...

  19. Enterprise Assessments Targeted Review of the Safety Basis at...

    Broader source: Energy.gov (indexed) [DOE]

    ... 421.1 is being updated to reflect the currently approved version of DOE-STD-1104-2014, which was issued in December 2014 and made mandatory for line management by DOE Order ...

  20. The Structural Basis of Cholesterol Activity in Membranes

    SciTech Connect (OSTI)

    Olsen, Brett N.; Bielska, Agata; Lee, Tiffany; Daily, Michael D.; Covey, Douglas F.; Schlesinger, Paul H.; Baker, Nathan A.; Ory, Daniel S.

    2013-10-15

    Although the majority of free cellular cholesterol is present in the plasma membrane, cholesterol homeostasis is principally regulated through sterol-sensing proteins that reside in the cholesterol-poor endoplasmic reticulum (ER). In response to acute cholesterol loading or depletion, there is rapid equilibration between the ER and plasma membrane cholesterol pools, suggesting a biophysical model in which the availability of plasma membrane cholesterol for trafficking to internal membranes modulates ER membrane behavior. Previous studies have predominantly examined cholesterol availability in terms of binding to extramembrane acceptors, but have provided limited insight into the structural changes underlying cholesterol activation. In this study, we use both molecular dynamics simulations and experimental membrane systems to examine the behavior of cholesterol in membrane bilayers. We find that cholesterol depth within the bilayer provides a reasonable structural metric for cholesterol availability and that this is correlated with cholesterol-acceptor binding. Further, the distribution of cholesterol availability in our simulations is continuous rather than divided into distinct available and unavailable pools. This data provide support for a revised cholesterol activation model in which activation is driven not by saturation of membrane-cholesterol interactions but rather by bulk membrane remodeling that reduces membrane-cholesterol affinity.

  1. Structural basis for the blockade of MATE multidrug efflux pumps

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Radchenko, Martha; Symersky, Jindrich; Nie, Rongxin; Lu, Min

    2015-08-06

    Multidrug and toxic compound extrusion (MATE) transporters underpin multidrug resistance by using the H+ or Na+ electrochemical gradient to extrude different drugs across cell membranes. MATE transporters can be further parsed into the DinF, NorM and eukaryotic subfamilies based on their amino-acid sequence similarity. Here we report the 3.0 Å resolution X-ray structures of a protonation-mimetic mutant of an H+-coupled DinF transporter, as well as of an H+-coupled DinF and a Na+-coupled NorM transporters in complexes with verapamil, a small-molecule pharmaceutical that inhibits MATE-mediated multidrug extrusion. Combining structure-inspired mutational and functional studies, we confirm the biological relevance of our crystalmore » structures, reveal the mechanistic differences among MATE transporters, and suggest how verapamil inhibits MATE-mediated multidrug efflux. Our findings offer insights into how MATE transporters extrude chemically and structurally dissimilar drugs and could inform the design of new strategies for tackling multidrug resistance.« less

  2. Structural basis for the blockade of MATE multidrug efflux pumps

    SciTech Connect (OSTI)

    Radchenko, Martha; Symersky, Jindrich; Nie, Rongxin; Lu, Min

    2015-08-06

    Multidrug and toxic compound extrusion (MATE) transporters underpin multidrug resistance by using the H+ or Na+ electrochemical gradient to extrude different drugs across cell membranes. MATE transporters can be further parsed into the DinF, NorM and eukaryotic subfamilies based on their amino-acid sequence similarity. Here we report the 3.0 Å resolution X-ray structures of a protonation-mimetic mutant of an H+-coupled DinF transporter, as well as of an H+-coupled DinF and a Na+-coupled NorM transporters in complexes with verapamil, a small-molecule pharmaceutical that inhibits MATE-mediated multidrug extrusion. Combining structure-inspired mutational and functional studies, we confirm the biological relevance of our crystal structures, reveal the mechanistic differences among MATE transporters, and suggest how verapamil inhibits MATE-mediated multidrug efflux. Our findings offer insights into how MATE transporters extrude chemically and structurally dissimilar drugs and could inform the design of new strategies for tackling multidrug resistance.

  3. Structural basis for DNA binding by replication initiator Mcm10

    SciTech Connect (OSTI)

    Warren, Eric M.; Vaithiyalingam, Sivaraja; Haworth, Justin; Greer, Briana; Bielinsky, Anja-Katrin; Chazin, Walter J.; Eichman, Brandt F.

    2009-06-30

    Mcm10 is an essential eukaryotic DNA replication protein required for assembly and progression of the replication fork. The highly conserved internal domain (Mcm10-ID) has been shown to physically interact with single-stranded (ss) DNA, DNA polymerase alpha, and proliferating cell nuclear antigen (PCNA). The crystal structure of Xenopus laevis Mcm10-ID presented here reveals a DNA binding architecture composed of an oligonucleotide/oligosaccharide-fold followed in tandem by a variant and highly basic zinc finger. NMR chemical shift perturbation and mutational studies of DNA binding activity in vitro reveal how Mcm10 uses this unique surface to engage ssDNA. Corresponding mutations in Saccharomyces cerevisiae result in increased sensitivity to replication stress, demonstrating the functional importance of DNA binding by this region of Mcm10 to replication. In addition, mapping Mcm10 mutations known to disrupt PCNA, polymerase alpha, and DNA interactions onto the crystal structure provides insight into how Mcm10 might coordinate protein and DNA binding within the replisome.

  4. Structural basis for substrate specificity in the Escherichia...

    Office of Scientific and Technical Information (OSTI)

    in the Escherichia coli maltose transport system Citation Details In-Document Search ... Sponsoring Org: NIH Country of Publication: United States Language: ENGLISH Word Cloud ...

  5. Elucidating the Molecular Basis and Regulation of Chromium (VI...

    Office of Scientific and Technical Information (OSTI)

    by Shewanella oneidensis MR-1. We have identified and characterized (in the case of DNA-binding response regulator SO2426 and a putative azoreductase SO3585) the genes and ...

  6. Los Alamos National Laboratory fission basis (Conference) | SciTech...

    Office of Scientific and Technical Information (OSTI)

    Los Alamos National Laboratory Publication Date: 2011-05-06 OSTI Identifier: 1063939 ... Symposium on Reactor Dosimetry ; May 22, 2011 ; Bretton Woods, NH Research Org: Los ...

  7. Structural basis of JAZ repression of MYC transcription factors...

    Office of Scientific and Technical Information (OSTI)

    Resource Type: Journal Article Resource Relation: Journal Name: Nature; Journal Volume: ... Word Cloud More Like This Full Text Journal Articles DOI: 10.1038nature14661 Select the ...

  8. The Three-Dimensional Structural Basis of Type II Hyperprolinemia...

    Office of Scientific and Technical Information (OSTI)

    Sponsoring Org: OTHERNIH Country of Publication: United States Language: ENGLISH Subject: 08 HYDROGEN; ALDEHYDES; CHAINS; CONFORMATIONAL CHANGES; CRYSTAL STRUCTURE; DEFECTS; ENZYMES; ...

  9. Structural basis of AMPK regulation by adenine nucleotides and glycogen

    SciTech Connect (OSTI)

    Li, Xiaodan; Wang, Lili; Zhou, X. Edward; Ke, Jiyuan; de Waal, Parker W.; Gu, Xin; Tan, M. H. Eileen; Wang, Dongye; Wu, Donghai; Xu, H. Eric; Melcher, Karsten

    2014-11-21

    AMP-activated protein kinase (AMPK) is a central cellular energy sensor and regulator of energy homeostasis, and a promising drug target for the treatment of diabetes, obesity, and cancer. Here we present low-resolution crystal structures of the human α1β2γ1 holo-AMPK complex bound to its allosteric modulators AMP and the glycogen-mimic cyclodextrin, both in the phosphorylated (4.05 Å) and non-phosphorylated (4.60 Å) state. In addition, we have solved a 2.95 Å structure of the human kinase domain (KD) bound to the adjacent autoinhibitory domain (AID) and have performed extensive biochemical and mutational studies. Altogether, these studies illustrate an underlying mechanism of allosteric AMPK modulation by AMP and glycogen, whose binding changes the equilibria between alternate AID (AMP) and carbohydrate-binding module (glycogen) interactions.

  10. Structural Basis of Transcription: Role of the Trigger Loop in...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    II active center. Cell 119, 481-489. Allison, L. A., Moyle, M., Shales, M., and Ingles, C. J. (1985). Extensive homology among the largest subunits of eukaryotic and...

  11. Elucidating the Molecular Basis and Regulation of Chromium (VI...

    Office of Scientific and Technical Information (OSTI)

    developed proteomic technology, in particular liquid chromatographymass spectrometry (LC-MS), in conjunction with conventional protein purification and characterization techniques. ...

  12. Laser design basis for the National Ignition Facility

    SciTech Connect (OSTI)

    Hunt, J.T.; Manes, K.R.; Murray, J.R.; Renard, P.A.; Sawicki, R.; Trenholme, J.B.; Williams, W.

    1994-06-01

    Controlled nuclear fusion initiated by highly intense laser beams has been the subject of experiment for many years. The National Ignition Facility (NIF) represents the culmination of design efforts to provide a laser facility that will successfully demonstrate fusion ignition in the laboratory. In this so-called inertial confinement approach, energetic driver beams (laser, X-ray, or charged particle) heat the outer surface of a spherical capsule containing deuterium and tritium (DT) fuel. As the capsule surface explosively evaporates, reaction pressure compresses the DT fuel causing the central core of the fuel to reach extreme density and temperature. When the central temperature is high enough, DT fusion reactions occur. The energy released from these reactions further heats the compressed fuel, and fusion burn propagates outward through the colder regions of the capsule much more rapidly than the inertially confined capsule can expand. The resulting fusion reactions yield many times more energy than was absorbed from the driver beams.

  13. Technical Cost Modeling - Life Cycle Analysis Basis for Program Focus |

    Broader source: Energy.gov (indexed) [DOE]

    | Department of Energy Newly Released Study Highlights Significant Utica Shale Potential Results from NETL-Sponsored Study Now Publically Available A pioneering study led by West Virginia University, and financially supported by the Energy Department's National Energy Technology Laboratory (NETL) and 14 industry members of the Utica Shale Appalachian Basin Exploration Consortium, indicates that the newly explored Utica Shale, which underlies the better-known Marcellus Shale, could hold far

  14. Structural basis for inhibition of DNA replication by aphidicolin

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Baranovskiy, A. G.; Babayeva, N. D.; Suwa, Y.; Gu, J.; Pavlov, Y. I.; Tahirov, T. H.

    2014-11-27

    Natural tetracyclic diterpenoid aphidicolin is a potent and specific inhibitor of B-family DNA polymerases, haltering replication and possessing a strong antimitotic activity in human cancer cell lines. Clinical trials revealed limitations of aphidicolin as an antitumor drug because of its low solubility and fast clearance from human plasma. The absence of structural information hampered the improvement of aphidicolin-like inhibitors: more than 50 modifications have been generated so far, but all have lost the inhibitory and antitumor properties. Here we report the crystal structure of the catalytic core of human DNA polymerase α (Pol α) in the ternary complex with anmore » RNA-primed DNA template and aphidicolin. The inhibitor blocks binding of dCTP by docking at the Pol α active site and by rotating the template guanine. The structure provides a plausible mechanism for the selectivity of aphidicolin incorporation opposite template guanine and explains why previous modifications of aphidicolin failed to improve its affinity for Pol α. With new structural information, aphidicolin becomes an attractive lead compound for the design of novel derivatives with enhanced inhibitory properties for B-family DNA polymerases.« less

  15. Structural basis for inhibition of DNA replication by aphidicolin

    SciTech Connect (OSTI)

    Baranovskiy, A. G.; Babayeva, N. D.; Suwa, Y.; Gu, J.; Pavlov, Y. I.; Tahirov, T. H.

    2014-11-27

    Natural tetracyclic diterpenoid aphidicolin is a potent and specific inhibitor of B-family DNA polymerases, haltering replication and possessing a strong antimitotic activity in human cancer cell lines. Clinical trials revealed limitations of aphidicolin as an antitumor drug because of its low solubility and fast clearance from human plasma. The absence of structural information hampered the improvement of aphidicolin-like inhibitors: more than 50 modifications have been generated so far, but all have lost the inhibitory and antitumor properties. Here we report the crystal structure of the catalytic core of human DNA polymerase α (Pol α) in the ternary complex with an RNA-primed DNA template and aphidicolin. The inhibitor blocks binding of dCTP by docking at the Pol α active site and by rotating the template guanine. The structure provides a plausible mechanism for the selectivity of aphidicolin incorporation opposite template guanine and explains why previous modifications of aphidicolin failed to improve its affinity for Pol α. With new structural information, aphidicolin becomes an attractive lead compound for the design of novel derivatives with enhanced inhibitory properties for B-family DNA polymerases.

  16. Technical Cost Modeling- Life Cycle Analysis Basis for Program Focus

    Broader source: Energy.gov [DOE]

    2010 DOE Vehicle Technologies and Hydrogen Programs Annual Merit Review and Peer Evaluation Meeting, June 7-11, 2010 -- Washington D.C.

  17. Adaptive Basis Selection and Dimensionality Reduction with Bayesian...

    Office of Scientific and Technical Information (OSTI)

    Publication Date: 2012-04-01 OSTI Identifier: 1078647 Report Number(s): SAND2012-2611C 447593 DOE Contract Number: AC04-94AL85000 Resource Type: Conference Resource Relation: ...

  18. Molecular Design Basis for Hydrogen Storage in Clathrate Hydrates

    SciTech Connect (OSTI)

    John, Vijay T; McPherson, Gary L; Ashbaugh, Hank; Johnes, Camille Y

    2013-06-28

    We attach a final technical report for the project. The report contains the list of all peer reviewed publications that have resulted from the contract. I will be happy to send the pdf files of the papers.

  19. Structural basis for bifunctional peptide recognition at human...

    Office of Scientific and Technical Information (OSTI)

    Raimund ; Fromme, Petra ; Tourw, Dirk ; Schiller, Peter W. ; Roth, Bryan L. ; Ballet, Steven ; Katritch, Vsevolod ; Stevens, Raymond C. ; Cherezov , Vadim 1 ; UNC) 2 ;...

  20. The structural basis for receptor recognition of human interleukin-18

    SciTech Connect (OSTI)

    Tsutsumi, Naotaka; Kimura, Takeshi; Arita, Kyohei; Ariyoshi, Mariko; Ohnishi, Hidenori; Yamamoto, Takahiro; Zuo, Xiaobing; Maenaka, Katsumi; Park, Enoch Y.; Kondo, Naomi; Shirakawa, Masahiro; Tochio, Hidehito; Kato, Zenichiro

    2014-12-15

    Interleukin (IL)-18 is a proinflammatory cytokine that belongs to the IL-1 family and plays an important role in inflammation. The uncontrolled release of this cytokine is associated with severe chronic inflammatory disease. IL-18 forms a signalling complex with the IL-18 receptor α (Rα) and β (Rβ) chains at the plasma membrane, which induces multiple inflammatory cytokines. Here, we present a crystal structure of human IL-18 bound to the two receptor extracellular domains. Generally, the receptors’ recognition mode for IL-18 is similar to IL-1β; however, certain notable differences were observed. The architecture of the IL-18 receptor second domain (D2) is unique among the other IL-1R family members, which presumably distinguishes them from the IL-1 receptors that exhibit a more promiscuous ligand recognition mode. The structures and associated biochemical and cellular data should aid in developing novel drugs to neutralize IL-8 activity.

  1. Enterprise Assessments Targeted Review of the Safety Basis at...

    Energy Savers [EERE]

    River Site F-Area Central Laboratory Facility (referred to as the FH Laboratory) to evaluate changes in the Safety Analysis Report (SAR) and Technical Safety Requirements (TSRs). ...

  2. Updated Costs (June 2011 Basis) for Selected Bituminous Baseline...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    has been eliminated in the current version. * Cases 1, 2, 13 and 14, Account 7.1 (Heat Recovery Steam Generator): The HRSG costs were re-calibrated using a series of more recent...

  3. Lawrence Livermore Site Office Safety Basis Self-Assessment Final...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DISCUSSION of RESULTS 1. LSO procedures and mechanisms address and implement 10 CFR 830 ... development and implementation of fully compliant documented safety analyses (DSAs) and ...

  4. Beyond Design Basis Events Analysis and Response Information...

    Broader source: Energy.gov (indexed) [DOE]

    Fact Finding Expert Mission of the Fukushima Dai-ichi Accident Following the Great East Japan Earthquake And Tsunami, June 16, 2011 INPO Special Report - Lessons Learned from the...

  5. Enterprise Assessments Review of the Delegation of Safety Basis...

    Energy Savers [EERE]

    4 5.2 Office of Nuclear Energy ... 7 5.3 Office of Environmental Management ......9 5.4 National Nuclear Security Administration ...

  6. Technical Cost Modeling - Life Cycle Analysis Basis for Program...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    reductions in GHG, criteria pollutants and acidification gases and * Development of LCA framework based on ISO standards and LCA technical reports such as 14040, 14044, and...

  7. Theoretical Basis for the Design of a DWPF Evacuated Canister

    SciTech Connect (OSTI)

    Routt, K.R.

    2001-09-17

    This report provides the theoretical bases for use of an evacuated canister for draining a glass melter. Design recommendations are also presented to ensure satisfactory performance in future tests of the concept.

  8. TECHNICAL BASIS DOCUMENT NO. 1: CLIMATE AND INFILTRATION

    SciTech Connect (OSTI)

    NA

    2004-05-01

    For the past 20 years, extensive field, laboratory, and modeling investigations have been performed at Yucca Mountain, which have led to the development of a number of conceptual models of infiltration and climate for the Yucca Mountain region around the repository site (Flint, A.L. et al. 2001; Wang and Bodvarsson 2003). Evaluating the amount of infiltrating water entering the subsurface is important, because this water may affect the percolation flux, which, in turn, controls seepage into the waste emplacement drifts and radionuclide transport from the repository to the water table. Forecasting of climatic data indicates that during the next 10,000 years at Yucca Mountain, the present-day climate should persist for 400 to 600 years, followed by a warmer and much wetter monsoon climate for 900 to 1,400 years, and by a cooler and wetter glacial-transition climate for the remaining 8,000 to 8,700 years. The analysis of climatic forecasting indicates that long-term climate conditions are generally predictable from a past climate sequence, while short-term climate conditions and weather predictions may be more variable and uncertain. The use of past climate sequences to bound future climate sequences involves several types of uncertainties, such as (1) uncertainty in the timing of future climate, (2) uncertainty in the methodology of climatic forecasting, and (3) uncertainty in the earth's future physical processes. Some of the uncertainties of the climatic forecasting are epistemic (reducible) and aleatoric (irreducible). Because of the size of the model domain, INFIL treats many flow processes in a simplified manner. For example, uptake of water by roots occurs according to the ''distributed model'', in which available water in each soil layer is withdrawn in proportion to the root density in that layer, multiplied by the total evapotranspirative demand. Runoff is calculated simply as the excess of precipitation over a sum of infiltration and water storage in the root zone. More significantly, water movement throughout the soil profile is treated according to the bucket model, in which the amount of water that moves down from one layer to the next is equal to the mass of water in excess of field capacity in the upper layer. The development of a numerical model of infiltration involves a number of abstractions and simplifications to represent the complexity of environmental conditions at Yucca Mountain, such as the arid climate, mountain-type topography, heterogeneous soils and fractured rock, and irregular soil-rock interface.

  9. Development of Guidance for Analysis of Beyond Design Basis Events

    Broader source: Energy.gov [DOE]

    Presenter: Dr. James O'Brien, Director, Office of Nuclear Safety, Office of Health, Safety and Security, US Department of Energy

  10. The structural basis for receptor recognition of human interleukin-18

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tsutsumi, Naotaka; Kimura, Takeshi; Arita, Kyohei; Ariyoshi, Mariko; Ohnishi, Hidenori; Yamamoto, Takahiro; Zuo, Xiaobing; Maenaka, Katsumi; Park, Enoch Y.; Kondo, Naomi; et al

    2014-12-15

    Interleukin (IL)-18 is a proinflammatory cytokine that belongs to the IL-1 family and plays an important role in inflammation. The uncontrolled release of this cytokine is associated with severe chronic inflammatory disease. IL-18 forms a signalling complex with the IL-18 receptor α (Rα) and β (Rβ) chains at the plasma membrane, which induces multiple inflammatory cytokines. Here, we present a crystal structure of human IL-18 bound to the two receptor extracellular domains. Generally, the receptors’ recognition mode for IL-18 is similar to IL-1β; however, certain notable differences were observed. The architecture of the IL-18 receptor second domain (D2) is uniquemore » among the other IL-1R family members, which presumably distinguishes them from the IL-1 receptors that exhibit a more promiscuous ligand recognition mode. The structures and associated biochemical and cellular data should aid in developing novel drugs to neutralize IL-8 activity.« less

  11. Molecular Basis for the Recognition of Structurally Distinct...

    Office of Scientific and Technical Information (OSTI)

    Distinct Autoinducer Mimics by the Pseudomonas aeruginosa LasR Quorum-Sensing Signaling ... of infection and host immune resistance, significant efforts have been ...

  12. An accurate and efficient computational protocol for obtaining the complete basis set limits of the binding energies of water clusters at the MP2 and CCSD(T) levels of theory: Application to (H?O)m, m=2-6, 8, 11, 16 and 17

    SciTech Connect (OSTI)

    Miliordos, Evangelos; Xantheas, Sotiris S.

    2015-06-21

    We report MP2 and CCSD(T) binding energies with basis sets up to pentuple zeta quality for the m = 2-6, 8 clusters. Or best CCSD(T)/CBS estimates are -4.99 kcal/mol (dimer), -15.77 kcal/mol (trimer), -27.39 kcal/mol (tetramer), -35.9 0.3 kcal/mol (pentamer), -46.2 0.3 kcal/mol (prism hexamer), -45.9 0.3 kcal/mol (cage hexamer), -45.4 0.3 kcal/mol (book hexamer), -44.3 0.3 kcal/mol (ring hexamer), -73.0 0.5 kcal/mol (D2d octamer) and -72.9 0.5 kcal/mol (S4 octamer). We have found that the percentage of both the uncorrected (dimer) and BSSE-corrected (dimerCPe) binding energies recovered with respect to the CBS limit falls into a narrow range for each basis set for all clusters and in addition this range was found to decrease upon increasing the basis set. Relatively accurate estimates (within < 0.5%) of the CBS limits can be obtained when using the 2/3, 1/3 (for the AVDZ set) or the , (for the AVTZ, AVQZ and AV5Z sets) mixing ratio between dimere and dimerCPe. Based on those findings we propose an accurate and efficient computational protocol that can be used to estimate accurate binding energies of clusters at the MP2 (for up to 100 molecules) and CCSD(T) (for up to 30 molecules) levels of theory. This work was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences and Biosciences. Pacific Northwest National Laboratory (PNNL) is a multi program national laboratory operated for DOE by Battelle. This research also used resources of the National Energy Research Scientific Computing Center, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. AC02-05CH11231.

  13. Use of Air2Air Technology to Recover Fresh-Water from the Normal Evaporative Cooling Loss at Coal-Based Thermoelectric Power Plants

    SciTech Connect (OSTI)

    Ken Mortensen

    2009-06-30

    This program was undertaken to build and operate the first Air2Air{trademark} Water Conservation Cooling Tower at a power plant, giving a validated basis and capability for water conservation by this method. Air2Air{trademark} water conservation technology recovers a portion of the traditional cooling tower evaporate. The Condensing Module provides an air-to-air heat exchanger above the wet fill media, extracting the heat from the hot saturated moist air leaving in the cooling tower and condensing water. The rate of evaporate water recovery is typically 10%-25% annually, depending on the cooling tower location (climate).

  14. Technical Basis Document: A Statistical Basis for Interpreting Urinary Excretion of Plutonium Based on Accelerator Mass Spectrometry (AMS) for Selected Atoll Populations in the Marshall Islands

    SciTech Connect (OSTI)

    Bogen, K; Hamilton, T F; Brown, T A; Martinelli, R E; Marchetti, A A; Kehl, S R; Langston, R G

    2007-05-01

    We have developed refined statistical and modeling techniques to assess low-level uptake and urinary excretion of plutonium from different population group in the northern Marshall Islands. Urinary excretion rates of plutonium from the resident population on Enewetak Atoll and from resettlement workers living on Rongelap Atoll range from <1 to 8 {micro}Bq per day and are well below action levels established under the latest Department regulation 10 CFR 835 in the United States for in vitro bioassay monitoring of {sup 239}Pu. However, our statistical analyses show that urinary excretion of plutonium-239 ({sup 239}Pu) from both cohort groups is significantly positively associated with volunteer age, especially for the resident population living on Enewetak Atoll. Urinary excretion of {sup 239}Pu from the Enewetak cohort was also found to be positively associated with estimates of cumulative exposure to worldwide fallout. Consequently, the age-related trends in urinary excretion of plutonium from Marshallese populations can be described by either a long-term component from residual systemic burdens acquired from previous exposures to worldwide fallout or a prompt (and eventual long-term) component acquired from low-level systemic intakes of plutonium associated with resettlement of the northern Marshall Islands, or some combination of both.

  15. Order Module--DOE-STD-1104-2009, REVIEW AND APPROVAL OF NUCLEAR FACILITY SAFETY BASIS AND SAFETY DESIGN BASIS DOCUMENTS

    Broader source: Energy.gov [DOE]

    The familiar level of this module is divided into two sections that are intended to provide only an overview of the material contained in DOE-STD-1104-2009, which should be consulted for complete...

  16. Jet-engine-based units for cleaning transport media and thawing frozen soil at mining, metallurgical, and transportation facilities

    SciTech Connect (OSTI)

    Khechuev, Y.D.

    2008-01-15

    In recent years, it has become much more difficult to deal with the adhesion and freezing of moist overburden or soil during mining and transport operations due to the increase in the volume of the various materials being mined and transported - coal, ore, fluxes, structural materials, etc. The most productive and effective methods to deal with the sticking and freezing of soil and rock are gas dynamic methods. These methods employ high-speed jets of hot gases from jet engines and can be 15-30 times more productive than mechanical methods and machinery. Proceeding on the basis of calculations, completed studies, and field tests, the Gortekhtrans Department of Research Institute for Problems of the Kursk Magnetic Anomaly (NIIKMA) has developed several highly efficient units that employ this technology.

  17. Improvement to Air2Air Technology to Reduce Fresh-Water Evaporative Cooling Loss at Coal-Based Thermoelectric Power Plants

    SciTech Connect (OSTI)

    Ken Mortensen

    2011-12-31

    This program was undertaken to enhance the manufacturability, constructability, and cost of the Air2Air{TM} Water Conservation and Plume Abatement Cooling Tower, giving a validated cost basis and capability. Air2Air{TM} water conservation technology recovers a portion of the traditional cooling tower evaporate. The Condensing Module provides an air-to-air heat exchanger above the wet fill media, extracting the heat from the hot saturated moist air leaving in the cooling tower and condensing water. The rate of evaporate water recovery is typically 10% - 25% annually, depending on the cooling tower location (climate). This program improved the efficiency and cost of the Air2Air{TM} Water Conservation Cooling Tower capability, and led to the first commercial sale of the product, as described.

  18. Technical basis for radiological release of Grand Junction Office Building 2. Volume 1, dose assessment

    SciTech Connect (OSTI)

    Morris, R.; Warga, J.; Thorne, D.

    1997-07-01

    Building 2 on the US Department of Energy (DOE) Grand Junction Office (GJO) site is part of the GJO Remedial Action Program (GJORAP). During evaluation of Building 2 for determination of radiological release disposition, some inaccessible surface contamination measurements were detected to be greater than the generic surface contamination guidelines of DOE Order 5400.5 (which are functionally equivalent to US Nuclear Regulatory Commission [NRC] Regulatory Guide 1.86). Although the building is nominal in size, it houses the site telecommunications system, that is critical to continued GJO operations, and demolition is estimated at $1.9 million. Because unrestricted release under generic surface contamination guidelines is cost-prohibitive, supplemental standards consistent with DOE Order 5400.5 are being pursued. This report describes measurements and dose analysis modeling efforts to evaluate the radiation dose to members of the public who might occupy or demolish Building 2, a 2,480 square-foot (ft) building constructed in 1944. The north portion of the building was used as a shower facility for Manhattan Project uranium-processing mill workers and the south portion was a warehouse. Many originally exposed surfaces are no longer accessible for contamination surveys because expensive telecommunications equipment have been installed on the floors and mounted on panels covering the walls. These inaccessible surfaces are contaminated above generic contamination limits.

  19. Assessing Beyond Design Basis Seismic Events and Implications on Seismic Risk

    Broader source: Energy.gov [DOE]

    Presenter: Jeffrey Kimball, Technical Specialist (Seismologist) Defense Nuclear Facilities Safety Board

  20. Performance Indicators for Uranium Bioremediation in the Subsurface: Basis and Assessment

    SciTech Connect (OSTI)

    Long, Philip E.; Yabusaki, Steven B.

    2006-12-29

    The purpose of this letter report is to identify performance indicators for in situ engineered bioremediation of subsurface uranium (U) contamination. This report focuses on in situ treatment of groundwater by biostimulation of extant in situ microbial populations (see http://128.3.7.51/NABIR/generalinfo/primers_guides/03_NABIR_primer.pdf for background information on bioremediation of metals and radionuclides). The treatment process involves amendment of the subsurface with an electron donor such as acetate, lactate, ethanol or other organic compound such that in situ microorganisms mediate the reduction of U(VI) to U(IV). U(VI) precipitates as uraninite or other insoluble U phase. Uranium is thus immobilized in place by such processes and is subject to reoxidation that may remobilize the reduced uranium. Related processes include augmenting the extant subsurface microbial populations, addition of electron acceptors, and introduction of chemically reducing materials such as zero-valent Fe. While metrics for such processes may be similar to those for in situ biostimulation, these related processes are not directly in the scope of this letter report.

  1. Molecular basis of endosomal-membrane association for the dengue virus envelope protein

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Rogers, David M.; Kent, Michael S.; Rempe, Susan B.

    2015-01-02

    Dengue virus is coated by an icosahedral shell of 90 envelope protein dimers that convert to trimers at low pH and promote fusion of its membrane with the membrane of the host endosome. We provide the first estimates for the free energy barrier and minimum for two key steps in this process: host membrane bending and protein–membrane binding. Both are studied using complementary membrane elastic, continuum electrostatics and all-atom molecular dynamics simulations. The predicted host membrane bending required to form an initial fusion stalk presents a 22–30 kcal/mol free energy barrier according to a constrained membrane elastic model. Combined continuummore »and molecular dynamics results predict a 15 kcal/mol free energy decrease on binding of each trimer of dengue envelope protein to a membrane with 30% anionic phosphatidylglycerol lipid. The bending cost depends on the preferred curvature of the lipids composing the host membrane leaflets, while the free energy gained for protein binding depends on the surface charge density of the host membrane. The fusion loop of the envelope protein inserts exactly at the level of the interface between the membrane's hydrophobic and head-group regions. As a result, the methods used in this work provide a means for further characterization of the structures and free energies of protein-assisted membrane fusion.« less

  2. Structural Basis for Microcin C7 Inactivation by the MccE Acetyltransf...

    Office of Scientific and Technical Information (OSTI)

    McC is synthesized as a heptapeptide-nucleotide conjugate, which is processed by cellular peptidases within target strains to yield the biologically active compound. As unwanted ...

  3. Review of Requirements and Capabilities for Analyzing and Responding to Beyond Design Basis Events

    Office of Energy Efficiency and Renewable Energy (EERE)

    This report describes the opportunities for improvement identified in DOE’s review, and provides recommendations for short-term and long-term actions for improving safety at DOE nuclear facilities.

  4. Savannah River Site Basis for Section 3116 Determination for Salt Waste Disposal

    Broader source: Energy.gov [DOE]

    The Department of Energy (DOE) published in the Federal Register (January 24, 2006), a Notice of Availability of Section 3116 Determination for Salt Waste Disposal at the Savannah River Site.

  5. Early Site Permit Demonstration Program: Guidelines for determining design basis ground motions. Volume 1

    SciTech Connect (OSTI)

    Not Available

    1993-03-18

    This report develops and applies a methodology for estimating strong earthquake ground motion. The motivation was to develop a much needed tool for use in developing the seismic requirements for structural designs. An earthquake`s ground motion is a function of the earthquake`s magnitude, and the physical properties of the earth through which the seismic waves travel from the earthquake fault to the site of interest. The emphasis of this study is on ground motion estimation in Eastern North America (east of the Rocky Mountains), with particular emphasis on the Eastern United States and southeastern Canada. Eastern North America is a stable continental region, having sparse earthquake activity with rare occurrences of large earthquakes. While large earthquakes are of interest for assessing seismic hazard, little data exists from the region to empirically quantify their effects. Therefore, empirically based approaches that are used for other regions, such as Western North America, are not appropriate for Eastern North America. Moreover, recent advances in science and technology have now made it possible to combine theoretical and empirical methods to develop new procedures and models for estimating ground motion. The focus of the report is on the attributes of ground motion in Eastern North America that are of interest for the design of facilities such as nuclear power plants. Specifically considered are magnitudes M from 5 to 8, distances from 0 to 500 km, and frequencies from 1 to 35 Hz.

  6. Structural basis for suppression of hypernegative DNA supercoiling by E. coli topoisomerase I

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Tan, Kemin; Zhou, Qingxuan; Cheng, Bokun; Zhang, Zhongtao; Joachimiak, Andrzej; Tse-Dinh, Yuk -Ching

    2015-10-20

    Escherichia coli topoisomerase I has an essential function in preventing hypernegative supercoiling of DNA. A full length structure of E. coli topoisomerase I reported here shows how the C-terminal domains bind single-stranded DNA (ssDNA) to recognize the accumulation of negative supercoils in duplex DNA. These C-terminal domains of E. coli topoisomerase I are known to interact with RNA polymerase, and two flexible linkers within the C-terminal domains may assist in the movement of the ssDNA for the rapid removal of transcription driven negative supercoils. The structure has also unveiled for the first time how the 4-Cys zinc ribbon domain andmore » zinc ribbon-like domain bind ssDNA with primarily π -stacking interactions. Finally, this novel structure, in combination with new biochemical data, provides important insights into the mechanism of genome regulation by type IA topoisomerases that is essential for life, as well as the structures of homologous type IA TOP3α and TOP3β from higher eukaryotes that also have multiple 4-Cys zinc ribbon domains required for their physiological functions.« less

  7. Precision wood particle feedstocks with retained moisture contents of greater than 30% dry basis

    DOE Patents [OSTI]

    Dooley, James H; Lanning, David N

    2014-10-28

    Wood particles having fibers aligned in a grain, wherein: the wood particles are characterized by a length dimension (L) aligned substantially parallel to the grain, a width dimension (W) normal to L and aligned cross grain, and a height dimension (H) normal to W and L; the L.times.H dimensions define two side surfaces characterized by substantially intact longitudinally arrayed fibers; the W.times.H dimensions define two cross-grain end surfaces characterized individually as aligned either normal to the grain or oblique to the grain; the L.times.W dimensions define two substantially parallel top and bottom surfaces; and, a majority of the W.times.H surfaces in the mixture of wood particles have end checking.

  8. Integrated Safety Management System as the Basis for Work Planning and Control for Research and Development

    Broader source: Energy.gov [DOE]

    Presenters: Rich Davies, Kami Lowry, Mike Schlender, Ted Pietrok, Pacific Northwest National Laboratory and Pacific Northwest Site Office

  9. Structural basis of cargo membrane protein discrimination by the human COPII coat machinery

    SciTech Connect (OSTI)

    Mancias, Joseph D.; Goldberg, Jonathan

    2008-11-18

    Genomic analysis shows that the increased complexity of trafficking pathways in mammalian cells involves an expansion of the number of SNARE, Rab and COP proteins. Thus, the human genome encodes four forms of Sec24, the cargo selection subunit of the COPII vesicular coat, and this is proposed to increase the range of cargo accommodated by human COPII-coated vesicles. In this study, we combined X-ray crystallographic and biochemical analysis with functional assays of cargo packaging into COPII vesicles to establish molecular mechanisms for cargo discrimination by human Sec24 subunits. A conserved IxM packaging signal binds in a surface groove of Sec24c and Sec24d, but the groove is occluded in the Sec24a and Sec24b subunits. Conversely, LxxLE class transport signals and the DxE signal of VSV glycoprotein are selectively bound by Sec24a and Sec24b subunits. A comparative analysis of crystal structures of the four human Sec24 isoforms establishes the structural determinants for discrimination among these transport signals, and provides a framework to understand how an expansion of coat subunits extends the range of cargo proteins packaged into COPII-coated vesicles.

  10. Structural Basis of Cargo Membrane Protein Discrimination by the Human COPll Coat Machinery

    SciTech Connect (OSTI)

    Mancias, J.; Goldberg, J

    2008-01-01

    Genomic analysis shows that the increased complexity of trafficking pathways in mammalian cells involves an expansion of the number of SNARE, Rab and COP proteins. Thus, the human genome encodes four forms of Sec24, the cargo selection subunit of the COPII vesicular coat, and this is proposed to increase the range of cargo accommodated by human COPII-coated vesicles. In this study, we combined X-ray crystallographic and biochemical analysis with functional assays of cargo packaging into COPII vesicles to establish molecular mechanisms for cargo discrimination by human Sec24 subunits. A conserved IxM packaging signal binds in a surface groove of Sec24c and Sec24d, but the groove is occluded in the Sec24a and Sec24b subunits. Conversely, LxxLE class transport signals and the DxE signal of VSV glycoprotein are selectively bound by Sec24a and Sec24b subunits. A comparative analysis of crystal structures of the four human Sec24 isoforms establishes the structural determinants for discrimination among these transport signals, and provides a framework to understand how an expansion of coat subunits extends the range of cargo proteins packaged into COPII-coated vesicles.

  11. Structural Basis for Near Unity Quantum Yield Core/Shell Nanostructures

    SciTech Connect (OSTI)

    McBride, James; Treadway, Joe; Pennycook, Stephen J; Rosenthal, Sandra

    2006-01-01

    Aberration-corrected Z-contrast scanning transmission electron microscopy of core/shell nanocrystals shows clear correlations between structure and quantum efficiency. Uniform shell coverage is obtained only for a graded CdS/ZnS shell material and is found to be critical to achieving near 100% quantum yield. The sublattice sensitivity of the images confirms that preferential growth takes place on the anion-terminated surfaces. This explains the three-dimensional "nanobullet" shape observed in the case of core/shell nanorods.

  12. Technical basis for installation of the double shell tank exhaust flow monitoring systems

    SciTech Connect (OSTI)

    Willingham, W.E., Fluor Daniel Hanford

    1997-03-11

    This document presents the technical bases for installation of flow meters on the ventilation exhaust ducts of the flammable gas watch list double shell tanks (241-AN-103, 241-AN-104, 241-AN-105, 241-AN-107, 241-AW-101 and 241-SY-103), the saltwell receiver tanks (241-AN-101 and 241-SY-102) and the cross-site receiver tank (241-AP-104).

  13. Possibility of creating weldable alloys on the basis of the system Al-Cu-Li

    SciTech Connect (OSTI)

    Fridlyander, I.N.; Drits, A.M.; Krymova, T.V.

    1992-03-01

    The weldability of alloys of the system Al-Cu-Li is studied and promising alloying ranges are established for weldable alloys: 5.5-6.5% Cu, 0.8-1.4% Li and 2.5-3.5% Cu, 1.9-2.5% Li. The positive effect of adding small amounts of scandium on the weldability and mechanical properties of alloys of the system Al-Cu-Li is demonstrated. Properties at normal and cryogenic temperatures are given for a new weldable alloy 1460. 7 refs., 6 figs., 2 tabs.

  14. Data Standards for Omics Data: the Basis of Data Sharing and Reuse.

    SciTech Connect (OSTI)

    Chervitz, Stephen A.; Deutsch, Eric W.; Field, Dawn; Parkinson, Helen E.; Quackenbush, John; Rocca-Serra, Philippe; Sansone, Susanna A.; Stoeckert, Christian Jr. J.; Taylor, Chris F.; Taylor, Ronald C.; Ball, Catherine A.

    2011-11-01

    To facilitate sharing of Omics data, many groups of scientists have been working to establish the relevant data standards. The main components of data sharing standards are experiment description standards, data exchange standards, terminology standards, and experiment execution standards. Here we provide a survey of existing and emerging standards that are intended to assist the free and open exchange of large-format data.

  15. Genome-scale model reveals metabolic basis of biomass partitioning in a model diatom

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Levering, Jennifer; Broddrick, Jared; Dupont, Christopher L.; Peers, Graham; Beeri, Karen; Mayers, Joshua; Gallina, Alessandra A.; Allen, Andrew E.; Palsson, Bernhard O.; Zengler, Karsten; et al

    2016-05-06

    Diatoms are eukaryotic microalgae that contain genes from various sources, including bacteria and the secondary endosymbiotic host. Due to this unique combination of genes, diatoms are taxonomically and functionally distinct from other algae and vascular plants and confer novel metabolic capabilities. Based on the genome annotation, we performed a genome-scale metabolic network reconstruction for the marine diatom Phaeodactylum tricornutum. Due to their endosymbiotic origin, diatoms possess a complex chloroplast structure which complicates the prediction of subcellular protein localization. Based on previous work we implemented a pipeline that exploits a series of bioinformatics tools to predict protein localization. The manually curatedmore » reconstructed metabolic network iLB1027_lipid accounts for 1,027 genes associated with 4,456 reactions and 2,172 metabolites distributed across six compartments. To constrain the genome-scale model, we determined the organism specific biomass composition in terms of lipids, carbohydrates, and proteins using Fourier transform infrared spectrometry. Our simulations indicate the presence of a yet unknown glutamine-ornithine shunt that could be used to transfer reducing equivalents generated by photosynthesis to the mitochondria. Furthermore, the model reflects the known biochemical composition of P. tricornutum in defined culture conditions and enables metabolic engineering strategies to improve the use of P. tricornutum for biotechnological applications.« less

  16. Basis for low beam loss in the high-current APT linac

    SciTech Connect (OSTI)

    Wangler, T.P.; Gray, E.R.; Krawczyk, F.L.; Kurennoy, S.S.; Lawrence, G.P.; Ryne, R.D.; Crandall, K.R.

    1998-12-31

    The present evidence that the APT proton linac design will meet its goal of low beam loss operation. The conclusion has three main bases: (1) extrapolation from the understanding of the performance of the 800-MeV LANSCE proton linac at Los Alamos, (2) the theoretical understanding of the dominant halo-forming mechanism in the APT accelerator from physics models and multiparticle simulations, and (3) the conservative approach and key principles underlying the design of the APT linac, which are aimed at minimizing beam halo and providing large apertures to reduce beam loss to a very low value.

  17. Structural basis of HIV-1 capsid recognition by PF74 and CPSF6

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Bhattacharya, Akash; Alam, Steven L.; Fricke, Thomas; Zadrozny, Kaneil; Sedzicki, Jaroslaw; Taylor, Alexander B.; Demeler, Borries; Pornillos, Owen; Ganser-Pornillos, Barbie K.; Diaz-Griffero, Felipe; et al

    2014-12-17

    Upon infection of susceptible cells by HIV-1, the conical capsid formed by ~250 hexamers and 12 pentamers of the CA protein is delivered to the cytoplasm. In this study, the capsid shields the RNA genome and proteins required for reverse transcription. In addition, the surface of the capsid mediates numerous host–virus interactions, which either promote infection or enable viral restriction by innate immune responses. In the intact capsid, there is an intermolecular interface between the N-terminal domain (NTD) of one subunit and the C-terminal domain (CTD) of the adjacent subunit within the same hexameric ring. The NTD–CTD interface is criticalmore » for capsid assembly, both as an architectural element of the CA hexamer and pentamer and as a mechanistic element for generating lattice curvature. Here we report biochemical experiments showing that PF-3450074 (PF74), a drug that inhibits HIV-1 infection, as well as host proteins cleavage and polyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer with at least 10-fold higher affinities compared with nonassembled CA or isolated CA domains. The crystal structure of PF74 in complex with the CA hexamer reveals that PF74 binds in a preformed pocket encompassing the NTD–CTD interface, suggesting that the principal inhibitory target of PF74 is the assembled capsid. Likewise, CPSF6 binds in the same pocket. Given that the NTD–CTD interface is a specific molecular signature of assembled hexamers in the capsid, binding of NUP153 at this site suggests that key features of capsid architecture remain intact upon delivery of the preintegration complex to the nucleus.« less

  18. Structural basis for bifunctional peptide recognition at human δ-opioid receptor

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Fenalti, Gustavo; Zatsepin, Nadia A.; Betti, Cecilia; Giguere, Patrick; Han, Gye Won; Ishchenko, Andrii; Liu, Wei; Guillemyn, Karel; Zhang, Haitao; James, Daniel; et al

    2015-02-16

    Bi-functional μ- and δ- opioid receptor (OR) ligands are potential therapeutic alternatives to alkaloid opiate analgesics with diminished side effects. We solved the structure of human δ-OR bound to the bi-functional δ-OR antagonist and μ-OR agonist tetrapeptide H-Dmt-Tic-Phe-Phe-NH2 (DIPP-NH2) by serial femtosecond crystallography, revealing a cis-peptide bond between H-Dmt and Tic. In summary, the observed receptor-peptide interactions are critical to understand the pharmacological profiles of opioid peptides, and to develop improved analgesics.

  19. Basis for in-situ geomechanical testing at the Yucca Mountain site

    SciTech Connect (OSTI)

    Board, M.

    1989-07-01

    This report presents an analysis of the in-situ geomechanical testing needs for the Exploratory Shaft (ES) test facility at the Yucca Mountain site in Nevada. The testing needs are derived from 10CFR60 regulations and simple thermomechanical canister- and room-scale numerical studies. The testing approach suggested is based on an ``iterative`` procedure of full-scale testing combined with numerical and empirical modeling. The testing suggested is based heavily on demonstration of excavation and thermal loading of full-scale repository excavations. Numerical and/or empirical models are compared to the full-scale response, allowing for adjustment of the model and evaluation of confidence in their predictive ability. Additional testing may be specified if confidence in prediction of the rock mass response is low. It is suggested that extensive drifting be conducted within the proposed repository area, including exploration of the bounding Drill Hole Wash and Imbricate fault structures, as well as the Ghost Dance fault. This approach is opposed to an a priori statistical specification of a number of ``point`` tests which attempt to measure a given property at a specific location. 40 refs., 49 figs., 6 tabs.

  20. Nuclear Regulatory Commission Handling of Beyond Design Basis Events for Nuclear Power Reactors

    Broader source: Energy.gov [DOE]

    Presenter: Bill Reckley, Chief, Policy and Support Branch, Japan Lessons-Learned Project Directorate, Office of Nuclear Reactor Regulation, US Nuclear Regulatory Commission US Nuclear Regulatory Commission

  1. Structural basis for specificity of TGF[beta] family receptor small molecule inhibitors

    SciTech Connect (OSTI)

    Ogunjimi, Abiodun A.; Zeqiraj, Elton; Ceccarelli, Derek F.; Sicheri, Frank; Wrana, Jeffrey L.; David, Laurent

    2012-07-24

    Transforming growth factor-{beta} (TGF{beta}) receptor kinase inhibitors have a great therapeutic potential. SB431542 is one of the mainly used kinase inhibitors of the TGF{beta}/Activin pathway receptors, but needs improvement of its EC{sub 50} (EC{sub 50} = 1 {mu}M) to be translated to clinical use. A key feature of SB431542 is that it specifically targets receptors from the TGF{beta}/Activin pathway but not the closely related receptors from the bone morphogenic proteins (BMP) pathway. To understand the mechanisms of this selectivity, we solved the crystal structure of the TGF{beta} type I receptor (T{beta}RI) kinase domain in complex with SB431542. We mutated T{beta}RI residues coordinating SB431542 to their counterparts in activin-receptor like kinase 2 (ALK2), a BMP receptor kinase, and tested the kinase activity of mutated T{beta}RI. We discovered that a Ser280Thr mutation yielded a T{beta}RI variant that was resistant to SB431542 inhibition. Furthermore, the corresponding Thr283Ser mutation in ALK2 yielded a BMP receptor sensitive to SB431542. This demonstrated that Ser280 is the key determinant of selectivity for SB431542. This work provides a framework for optimising the SB431542 scaffold to more potent and selective inhibitors of the TGF{beta}/Activin pathway.

  2. Turbulence-Turbine Interaction: The Basis for the Development of the TurbSim Stochastic Simulator

    SciTech Connect (OSTI)

    Kelley, N. D.

    2011-11-01

    A combination of taller wind turbines with more flexible rotors and towers operating in turbulent conditions that are not well understood is contributing to much higher than anticipated maintenance and repairs costs and is associated with lower energy production. This report documents evidence of this and offers the turbine designers an expanded tool that resolves many of these shortcomings.

  3. Radioactive and mixed waste - risk as a basis for waste classification. Symposium proceedings No. 2

    SciTech Connect (OSTI)

    1995-06-21

    The management of risks from radioactive and chemical materials has been a major environmental concern in the United states for the past two or three decades. Risk management of these materials encompasses the remediation of past disposal practices as well as development of appropriate strategies and controls for current and future operations. This symposium is concerned primarily with low-level radioactive wastes and mixed wastes. Individual reports were processed separately for the Department of Energy databases.

  4. Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization

    SciTech Connect (OSTI)

    Fairman, James Wesley; Wijerathna, Sanath Ranjan; Ahmad, Md Faiz; Xu, Hai; Nakano, Ryo; Jha, Shalini; Prendergast, Jay; Welin, R. Martin; Flodin, Susanne; Roos, Annette; Nordlund, Pr; Li, Zongli; Walz, Thomas; Dealwis, Chris Godfrey

    2011-07-25

    Ribonucleotide reductase (RR) is an {alpha}{sub n}{beta}{sub n} (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the {alpha}{sub 6}-{beta}{beta}'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

  5. Structural Basis for Allosteric Regulation of Human Ribonucleotide Reductase by Nucleotide-induced Oligomerization

    SciTech Connect (OSTI)

    J Fairman; S Wijerathna; M Ahmad; H Xu; R nakano; S jha; J Prendergast; R Welin; S Flodin; et al.

    2011-12-31

    Ribonucleotide reductase (RR) is an {alpha}{sub n}{beta}{sub n} (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the {alpha}{sub 6}-{beta}{beta}'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

  6. Draft Basis for Section 3116 Determination for Closure of F-Tank...

    Office of Environmental Management (EM)

    ... 1.1 Introduction In accordance with NDAA Section 3116, certain waste from reprocessing of spent nuclear fuel is not high- level waste if the Secretary of Energy, in consultation ...

  7. Technical basis for extending storage of the UK's advanced gas-cooled reactor fuel

    SciTech Connect (OSTI)

    Hambley, D.I.

    2013-07-01

    The UK Nuclear Decommissioning Agency has recently declared a date for cessation of reprocessing of oxide fuel from the UK's Advanced Gas-cooled Reactors (AGRs). This will fundamentally change the management of AGR fuel: from short term storage followed by reprocessing to long term fuel storage followed, in all likelihood, by geological disposal. In terms of infrastructure, the UK has an existing, modern wet storage asset that can be adapted for centralised long term storage of dismantled AGR fuel under the required pond water chemistry. No AGR dry stores exist, although small quantities of fuel have been stored dry as part of experimental programmes in the past. These experimental programmes have shown concerns about corrosion rates.

  8. Proceedings of the symposium on Scientific Basis for Nuclear Waste Management XXX

    SciTech Connect (OSTI)

    Dunn, Darrell; Poinssot, Christophe; Begg, Bruce

    2007-07-01

    Management of nuclear waste remains an important international topic that includes reprocessing of commercial nuclear fuel, waste-form design and development, storage and disposal packaging, the process of repository site selection, system design, and performance assessment. Requirements to manage and dispose of materials from the production of nuclear weapons, and the renewed interest in nuclear power, in particular through the Generation IV Forum and the Advanced Fuel Cycle Initiative, can be expected to increase the need for scientific advances in waste management. A broad range of scientific and engineering disciplines is necessary to provide safe and effective solutions and address complex issues. This volume offers an interdisciplinary perspective on materials-related issues associated with nuclear waste management programs. Invited and contributed papers cover a wide range of topics including studies on: spent fuel; performance assessment and models; waste forms for low- and intermediate-level waste; ceramic and glass waste forms for plutonium and high-level waste; radionuclides; containers and engineered barriers; disposal environments and site characteristics; and partitioning and transmutation.

  9. The Structural Basis for Tight Control of PP2A Methylation and...

    Office of Scientific and Technical Information (OSTI)

    Our studies suggested mechanisms of LCMT-1 in tight control of PP2A function, important ... Country of Publication: United States Language: ENGLISH Subject: 59 BASIC BIOLOGICAL ...

  10. Structural Basis of Preexisting Immunity to the 2009 H1N1 Pandemic Influenza Virus

    SciTech Connect (OSTI)

    Xu, Rui; Ekiert, Damian C.; Krause, Jens C.; Hai, Rong; Crowe, Jr., James E.; Wilson, Ian A.

    2010-05-25

    The 2009 H1N1 swine flu is the first influenza pandemic in decades. The crystal structure of the hemagglutinin from the A/California/04/2009 H1N1 virus shows that its antigenic structure, particularly within the Sa antigenic site, is extremely similar to those of human H1N1 viruses circulating early in the 20th century. The cocrystal structure of the 1918 hemagglutinin with 2D1, an antibody from a survivor of the 1918 Spanish flu that neutralizes both 1918 and 2009 H1N1 viruses, reveals an epitope that is conserved in both pandemic viruses. Thus, antigenic similarity between the 2009 and 1918-like viruses provides an explanation for the age-related immunity to the current influenza pandemic.

  11. Early Site Permit Demonstration Program: Guidelines for determining design basis ground motions. Volume 2, Appendices

    SciTech Connect (OSTI)

    Not Available

    1993-03-18

    This report develops and applies a methodology for estimating strong earthquake ground motion. The motivation was to develop a much needed tool for use in developing the seismic requirements for structural designs. An earthquake`s ground motion is a function of the earthquake`s magnitude, and the physical properties of the earth through which the seismic waves travel from the earthquake fault to the site of interest. The emphasis of this study is on ground motion estimation in Eastern North America (east of the Rocky Mountains), with particular emphasis on the Eastern United States and southeastern Canada. Eastern North America is a stable continental region, having sparse earthquake activity with rare occurrences of large earthquakes. While large earthquakes are of interest for assessing seismic hazard, little data exists from the region to empirically quantify their effects. The focus of the report is on the attributes of ground motion in Eastern North America that are of interest for the design of facilities such as nuclear power plants. This document, Volume II, contains Appendices 2, 3, 5, 6, and 7 covering the following topics: Eastern North American Empirical Ground Motion Data; Examination of Variance of Seismographic Network Data; Soil Amplification and Vertical-to-Horizontal Ratios from Analysis of Strong Motion Data From Active Tectonic Regions; Revision and Calibration of Ou and Herrmann Method; Generalized Ray Procedure for Modeling Ground Motion Attenuation; Crustal Models for Velocity Regionalization; Depth Distribution Models; Development of Generic Site Effects Model; Validation and Comparison of One-Dimensional Site Response Methodologies; Plots of Amplification Factors; Assessment of Coupling Between Vertical & Horizontal Motions in Nonlinear Site Response Analysis; and Modeling of Dynamic Soil Properties.

  12. The Arabidopsis lyrata genome sequence and the basis of rapid genome size change

    SciTech Connect (OSTI)

    Hu, Tina T.; Pattyn, Pedro; Bakker, Erica G.; Cao, Jun; Cheng, Jan-Fang; Clark, Richard M.; Fahlgren, Noah; Fawcett, Jeffrey A.; Grimwood, Jane; Gundlach, Heidrun; Haberer, Georg; Hollister, Jesse D.; Ossowski, Stephan; Ottilar, Robert P.; Salamov, Asaf A.; Schneeberger, Korbinian; Spannagl, Manuel; Wang, Xi; Yang, Liang; Nasrallah, Mikhail E.; Bergelson, Joy; Carrington, James C.; Gaut, Brandon S.; Schmutz, Jeremy; Mayer, Klaus F. X.; Van de Peer, Yves; Grigoriev, Igor V.; Nordborg, Magnus; Weigel, Detlef; Guo, Ya-Long

    2011-04-29

    In our manuscript, we present a high-quality genome sequence of the Arabidopsis thaliana relative, Arabidopsis lyrata, produced by dideoxy sequencing. We have performed the usual types of genome analysis (gene annotation, dN/dS studies etc. etc.), but this is relegated to the Supporting Information. Instead, we focus on what was a major motivation for sequencing this genome, namely to understand how A. thaliana lost half its genome in a few million years and lived to tell the tale. The rather surprising conclusion is that there is not a single genomic feature that accounts for the reduced genome, but that every aspect centromeres, intergenic regions, transposable elements, gene family number is affected through hundreds of thousands of cuts. This strongly suggests that overall genome size in itself is what has been under selection, a suggestion that is strongly supported by our demonstration (using population genetics data from A. thaliana) that new deletions seem to be driven to fixation.

  13. Structural Basis for Ligand Recognition and Discrimination of a Quorum-quenching Antibody

    SciTech Connect (OSTI)

    Kirchdoerfer, Robert N.; Garner, Amanda L.; Flack, Caralyn E.; Mee, Jenny M.; Horswill, Alexander R.; Janda, Kim D.; Kaufmann, Gunnar F.; Wilson, Ian A.

    2011-09-16

    In the postantibiotic era, available treatment options for severe bacterial infections caused by methicillin-resistant Staphylococcus aureus have become limited. Therefore, new and innovative approaches are needed to combat such life-threatening infections. Virulence factor expression in S. aureus is regulated in a cell density-dependent manner using 'quorum sensing,' which involves generation and secretion of autoinducing peptides (AIPs) into the surrounding environment to activate a bacterial sensor kinase at a particular threshold concentration. Mouse monoclonal antibody AP4-24H11 was shown previously to blunt quorum sensing-mediated changes in gene expression in vitro and protect mice from a lethal dose of S. aureus by sequestering the AIP signal. We have elucidated the crystal structure of the AP4-24H11 Fab in complex with AIP-4 at 2.5 {angstrom} resolution to determine its mechanism of ligand recognition. A key GluH95 provides much of the binding specificity through formation of hydrogen bonds with each of the four amide nitrogens in the AIP-4 macrocyclic ring. Importantly, these structural data give clues as to the interactions between the cognate staphylococcal AIP receptors AgrC and the AIPs, as AP4-24H11 {center_dot} AIP-4 binding recapitulates features that have been proposed for AgrC-AIP recognition. Additionally, these structural insights may enable the engineering of AIP cross-reactive antibodies or quorum quenching vaccines for use in active or passive immunotherapy for prevention or treatment of S. aureus infections.

  14. Structural Basis for Streptogramin B Resistance in Staphylococcus aureus by Virginiamycin B Lyase

    SciTech Connect (OSTI)

    Korczynska,M.; Mukhtar, T.; Wright, G.; Berghuis, A.

    2007-01-01

    The streptogramin combination therapy of quinupristin-dalfopristin (Synercid) is used to treat infections caused by bacterial pathogens, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. However, the effectiveness of this therapy is being compromised because of an increased incidence of streptogramin resistance. One of the clinically observed mechanisms of resistance is enzymatic inactivation of the type B streptogramins, such as quinupristin, by a streptogramin B lyase, i.e., virginiamycin B lyase (Vgb). The enzyme catalyzes the linearization of the cyclic antibiotic via a cleavage that requires a divalent metal ion. Here, we present crystal structures of Vgb from S. aureus in its apoenzyme form and in complex with quinupristin and Mg{sup 2+} at 1.65- and 2.8-{angstrom} resolution, respectively. The fold of the enzyme is that of a seven-bladed {beta}-propeller, although the sequence reveals no similarity to other known members of this structural family. Quinupristin binds to a large depression on the surface of the enzyme, where it predominantly forms van der Waals interactions. Validated by site-directed mutagenesis studies, a reaction mechanism is proposed in which the initial abstraction of a proton is facilitated by a Mg{sup 2+}-linked conjugated system. Analysis of the Vgb-quinupristin structure and comparison with the complex between quinupristin and its natural target, the 50S ribosomal subunit, reveals features that can be exploited for developing streptogramins that are impervious to Vgb-mediated resistance.

  15. Materials Data on BaSi23 (SG:223) by Materials Project

    SciTech Connect (OSTI)

    Kristin Persson

    2015-05-16

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  16. Genetic basis of resistance to trauma in inbred strains of mice

    SciTech Connect (OSTI)

    Radojicic, C.; Andric, B.; Simovic, M.; Dujic, A.; Marinkovic, D. )

    1990-02-01

    In this study the resistance to mechanical, thermal, and radiation trauma in four inbred strains of mice (AKR, BALB/c, CBA, and C57Bl/6) was compared with the degree of genetic resemblance, by analyzing the allozyme variabilities of these strains. It was shown that the highest degree of genetic resemblance was among CBA and AKR strains, which correlated with a similar degree of resistance to trauma. On the other hand, BALB/c and C57Bl/6 strains expressed significant differences, both genetically and with respect to the responses to trauma. The hypothesis is introduced that the genetic determination of the resistance to trauma is based on: (a) a polygenic control of general physiological homeostasis, with the possibility that (b) some specific genes or single loci may contribute more than others to such adaptations of the strains tested.

  17. Molecular basis of endosomal-membrane association for the dengue virus envelope protein

    SciTech Connect (OSTI)

    Rogers, David M.; Kent, Michael S.; Rempe, Susan B.

    2015-01-02

    Dengue virus is coated by an icosahedral shell of 90 envelope protein dimers that convert to trimers at low pH and promote fusion of its membrane with the membrane of the host endosome. We provide the first estimates for the free energy barrier and minimum for two key steps in this process: host membrane bending and protein–membrane binding. Both are studied using complementary membrane elastic, continuum electrostatics and all-atom molecular dynamics simulations. The predicted host membrane bending required to form an initial fusion stalk presents a 22–30 kcal/mol free energy barrier according to a constrained membrane elastic model. Combined continuum and molecular dynamics results predict a 15 kcal/mol free energy decrease on binding of each trimer of dengue envelope protein to a membrane with 30% anionic phosphatidylglycerol lipid. The bending cost depends on the preferred curvature of the lipids composing the host membrane leaflets, while the free energy gained for protein binding depends on the surface charge density of the host membrane. The fusion loop of the envelope protein inserts exactly at the level of the interface between the membrane's hydrophobic and head-group regions. As a result, the methods used in this work provide a means for further characterization of the structures and free energies of protein-assisted membrane fusion.

  18. DOE's Safety Bulletin No. 2011-01, Events Beyond Design Safety Basis Analysis, March 2011

    Broader source: Energy.gov [DOE]

    PURPOSE This Safety Alert provides information on a safety concern related to the identification and mitigation of events that may fall outside those analyzed in the documented safety analysis.

  19. Basis for and practical methods of controlling painting activities at the Sequoyah Nuclear Plant

    SciTech Connect (OSTI)

    Campbell, R.R.

    1997-08-01

    Sequoyah Nuclear Plant (SQN) follows the guidance presented in Regulatory Guide (R.G.) 1.52, {open_quotes}Design, Testing, and Maintenance Criteria for Atmospheric Cleanup System Air Filtration and Adsorption System Units of Light-Water-Cooled Nuclear Power Plants{close_quotes} in protecting its charcoal filter trains from the effects of painting and other chemical releases. SQN, as well as other nuclear facilities around the country, have the problem of how to address the issue of protection of Engineered Safety Feature (ESF) filter systems from degradation due to communication with airborne hydrocarbons (i.e., primarily paints and solvents). R.G. 1.52 (and a similar statement from R.G. 1.140) states in part,{open_quotes}Testing should be performed ... following painting, fire, or chemical release in any ventilation zone communicating with the system...,{close_quotes} and requires that a test be performed upon any kind of painting or chemical release. This is considered overly restrictive if the activity is minor and in a location remote from the charcoal filters. Charcoal filters used in air cleaning systems are required to filter out radioactive iodine from an airstream before its release from the plant to the environment. Charcoal filters will age with time because of their ability to adsorb many different types of material. This aging affects the charcoal by lowering its iodine retention efficiency, and therefore the charcoal needs to be protected from the effects of chemicals such as paint fumes. 14 refs., 3 tabs.

  20. Structure of P-Glycoprotein Reveals a Molecular Basis for Poly...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    for Poly-Specific Drug Binding figure 1 Figure 1. Structure of P-gp. Many forms of cancer fail to respond to chemotherapy by acquiring multidrug resistance (MDR), to which has...

  1. System and method for 100% moisture and basis weight measurement of moving paper

    DOE Patents [OSTI]

    Hernandez, Jose E.; Koo, Jackson C.

    2002-01-01

    A system for characterizing a set of properties for a moving substance are disclosed. The system includes: a first near-infrared linear array; a second near-infrared linear array; a first filter transparent to a first absorption wavelength emitted by the moving substance and juxtaposed between the substance and the first array; a second filter blocking the first absorption wavelength emitted by the moving substance and juxtaposed between the substance and the second array; and a computational device for characterizing data from the arrays into information on a property of the substance. The method includes the steps of: filtering out a first absorption wavelength emitted by a substance; monitoring the first absorption wavelength with a first near-infrared linear array; blocking the first wavelength from reaching a second near-infrared linear array; and characterizing data from the arrays into information on a property of the substance.

  2. Structural Basis for Putrescine Activation of Human S-Adenosylmethionine Decarboxylase

    SciTech Connect (OSTI)

    Bale, Shridhar; Lopez, Maria M.; Makhatadze, George I.; Fang, Qingming; Pegg, Anthony E.; Ealick, Steven E.

    2009-01-23

    Putrescine (1,4-diaminobutane) activates the autoprocessing and decarboxylation reactions of human S-adenosylmethionine decarboxylase (AdoMetDC), a critical enzyme in the polyamine biosynthetic pathway. In human AdoMetDC, putrescine binds in a buried pocket containing acidic residues Asp174, Glu178, and Glu256. The pocket is away from the active site but near the dimer interface; however, a series of hydrophilic residues connect the putrescine binding site and the active site. Mutation of these acidic residues modulates the effects of putrescine. D174N, E178Q, and E256Q mutants were expressed and dialyzed to remove putrescine and studied biochemically using X-ray crystallography, UV-CD spectroscopy, analytical ultracentrifugation, and ITC binding studies. The results show that the binding of putrescine to the wild type dimeric protein is cooperative. The D174N mutant does not bind putrescine, and the E178Q and E256Q mutants bind putrescine weakly with no cooperativity. The crystal structure of the mutants with and without putrescine and their complexes with S-adenosylmethionine methyl ester were obtained. Binding of putrescine results in a reorganization of four aromatic residues (Phe285, Phe315, Tyr318, and Phe320) and a conformational change in the loop 312-320. The loop shields putrescine from the external solvent, enhancing its electrostatic and hydrogen bonding effects. The E256Q mutant with putrescine added shows an alternate conformation of His243, Glu11, Lys80, and Ser229, the residues that link the active site and the putrescine binding site, suggesting that putrescine activates the enzyme through electrostatic effects and acts as a switch to correctly orient key catalytic residues.

  3. Microsoft Word - CR-091 Primary Basis of Cost Savings and Cost...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    of Cost Savings and Cost Savings Amount. The new configurations will only apply to acquisition document types, specifically: BPA Calls, Contracts, Delivery OrderTask Order,...

  4. DOE M&O Contractor Perspectives on Beyond Design Basis Event Analysis and Response

    Broader source: Energy.gov [DOE]

    Presenter: Ron Crone, Director Research Reactors Division UT-Battelle, LLC Oak Ridge National Laboratory

  5. Structural Basis of UV DNA-Damage Recognition by the DDB1-DDB2...

    Office of Scientific and Technical Information (OSTI)

    DDB2 to detect lesions refractory to detection by other damage surveillance proteins. ... Issue: (7) ; 12, 2008 Research Org: Advanced Photon Source (APS), Argonne National ...

  6. Basis for Identification of Disposal Options for R and D for...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    conditions on the design of the repository, the engineered barrier, and the waste. Salt, clayshale, and granitic rocks represent a reasonable cross-section of behavior....

  7. Structural Basis of Selective Ubiquitination of TRF1 by SCFFbx4...

    Office of Scientific and Technical Information (OSTI)

    Additional Journal Information: Journal Volume: 18; Journal Issue: 2; Journal ID: ISSN 1534-5807 Publisher: Elsevier Sponsoring Org: USDOE Country of Publication: Country unknown...

  8. Ion Fast Ignition-Establishing a Scientific Basis for Inertial Fusion Energy --- Final Report

    SciTech Connect (OSTI)

    Stephens, Richard Burnite; Foord, Mark N.; Wei, Mingsheng; Beg, Farhat N.; Schumacher, Douglass W.

    2013-10-31

    The Fast Ignition (FI) Concept for Inertial Confinement Fusion (ICF) has the potential to provide a significant advance in the technical attractiveness of Inertial Fusion Energy reactors. FI differs from conventional ?central hot spot? (CHS) target ignition by decoupling compression from heating: using a laser (or heavy ion beam or Z pinch) drive pulse (10?s of nanoseconds) to create a dense fuel and a second, much shorter (~10 picoseconds) high intensity pulse to ignite a small volume within the dense fuel. The compressed fuel is opaque to laser light. The ignition laser energy must be converted to a jet of energetic charged particles to deposit energy in the dense fuel. The original concept called for a spray of laser-generated hot electrons to deliver the energy; lack of ability to focus the electrons put great weight on minimizing the electron path. An alternative concept, proton-ignited FI, used those electrons as intermediaries to create a jet of protons that could be focused to the ignition spot from a more convenient distance. Our program focused on the generation and directing of the proton jet, and its transport toward the fuel, none of which were well understood at the onset of our program. We have developed new experimental platforms, diagnostic packages, computer modeling analyses, and taken advantage of the increasing energy available at laser facilities to create a self-consistent understanding of the fundamental physics underlying these issues. Our strategy was to examine the new physics emerging as we added the complexity necessary to use proton beams in an inertial fusion energy (IFE) application. From the starting point of a proton beam accelerated from a flat, isolated foil, we 1) curved it to focus the beam, 2) attached the foil to a superstructure, 3) added a side sheath to protect it from the surrounding plasma, and finally 4) studied the proton beam behavior as it passed through a protective end cap into plasma. We built up, as we proceeded, a self-consistent picture of the quasi-neutral plasma jet that is the proton beam that, for the first time, included the role of the hot electrons in shaping the jet. Controlling them?through design of the accelerating surface and its connection to the surrounding superstructure?is critical; their uniform spread across the proton accelerating area is vital, but their presence in the jet opposes focus; their electron flow away from the acceleration area reduces conversion efficiency but can also increase focusing ability. The understanding emerging from our work and the improved simulation tools we have developed allow designing structures that optimize proton beams for focused heating. Our findings include: ? The achievable focus of proton beams is limited by the thermal pressure gradient in the laser-generated hot electrons that drive the process. This bending can be suppressed using a controlled flow of hot electrons along the surrounding cone wall, which induces a local transverse focusing sheath electric field. The resultant (vacuum-focused) spot can meet IFE requirements. ? Confinement of laser-generated electrons to the proton accelerating area can be achieved by supporting targets on thin struts. That increases laser-to-proton conversion energy by ~50%. As noted above, confinement should not be total; necessary hot-electron leakage into the surrounding superstructure for proton focusing can be controlled by with the strut width/number. ? Proton jets are further modified as they enter the fuel through the superstructure?s end cap. They can generate currents during that transit that further focus the proton beams. We developed a new ion stopping module for LSP code that properly accounted for changes in stopping power with ionization (e.g. temperature), and will be using it in future studies. The improved understanding, new experimental platforms, and the self-consistent modeling capability allow researchers a new ability to investigate the interaction of large ion currents with warm dense matter. That is of direct importance to the creation and investiga

  9. Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Pennington, Luke F.; Tarchevskaya, Svetlana; Brigger, Daniel; Sathiyamoorthy, Karthik; Graham, Michelle T.; Nadeau, Kari Christine; Eggel, Alexander; Jardetzky, Theodore S.

    2016-05-19

    Omalizumab is a widely used therapeutic anti-IgE antibody. Here we report the crystal structure of the omalizumab–Fab in complex with an IgE-Fc fragment. This structure reveals the mechanism of omalizumab-mediated inhibition of IgE interactions with both high- and low-affinity IgE receptors, and explains why omalizumab selectively binds free IgE. The structure of the complex also provides mechanistic insight into a class of disruptive IgE inhibitors that accelerate the dissociation of the high-affinity IgE receptor from IgE. We use this structural data to generate a mutant IgE-Fc fragment that is resistant to omalizumab binding. Treatment with this omalizumab-resistant IgE-Fc fragment, inmore » combination with omalizumab, promotes the exchange of cell-bound full-length IgE with omalizumab-resistant IgE-Fc fragments on human basophils. Furthermore, this combination treatment also blocks basophil activation more efficiently than either agent alone, providing a novel approach to probe regulatory mechanisms underlying IgE hypersensitivity with implications for therapeutic interventions.« less

  10. Order Module--NNSA Orders Self-Study Program Safety Basis Documentation

    Office of Energy Efficiency and Renewable Energy (EERE)

    The familiar level of this module is divided into five sections. In the first section, we will discuss the regulation. In the second, third, and fourth sections, we will discuss the U. Department...

  11. Waste Encapsulation and Storage Facility (WESF) Basis for Interim Operation (BIO)

    SciTech Connect (OSTI)

    COVEY, L.I.

    2000-11-28

    The Waste Encapsulation and Storage Facility (WESF) is located in the 200 East Area adjacent to B Plant on the Hanford Site north of Richland, Washington. The current WESF mission is to receive and store the cesium and strontium capsules that were manufactured at WESF in a safe manner and in compliance with all applicable rules and regulations. The scope of WESF operations is currently limited to receipt, inspection, decontamination, storage, and surveillance of capsules in addition to facility maintenance activities. The capsules are expected to be stored at WESF until the year 2017, at which time they will have been transferred for ultimate disposition. The WESF facility was designed and constructed to process, encapsulate, and store the extracted long-lived radionuclides, {sup 90}Sr and {sup 137}Cs, from wastes generated during the chemical processing of defense fuel on the Hanford Site thus ensuring isolation of hazardous radioisotopes from the environment. The construction of WESF started in 1971 and was completed in 1973. Some of the {sup 137}Cs capsules were leased by private irradiators or transferred to other programs. All leased capsules have been returned to WESF. Capsules transferred to other programs will not be returned except for the seven powder and pellet Type W overpacks already stored at WESF.

  12. The crystal structure of human GlnRS provides basis for the development of neurological disorders

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ognjenovic, Jana; Wu, Jiang; Matthies, Doreen; Baxa, Ulrich; Subramaniam, Sriram; Ling, Jiqiang; Simonovic, Miljan

    2016-02-10

    Cytosolic glutaminyl-tRNA synthetase (GlnRS) is the singular enzyme responsible for translation of glutamine codons. Compound heterozygous mutations in GlnRS cause severe brain disorders by a poorly understood mechanism. Herein, we present crystal structures of the wild type and two pathological mutants of human GlnRS, which reveal, for the first time, the domain organization of the intact enzyme and the structure of the functionally important N-terminal domain (NTD). Pathological mutations mapping in the NTD alter the domain structure, and decrease catalytic activity and stability of GlnRS, whereas missense mutations in the catalytic domain induce misfolding of the enzyme. Our results suggestmore » that the reduced catalytic efficiency and a propensity of GlnRS mutants to misfold trigger the disease development. As a result, this report broadens the spectrum of brain pathologies elicited by protein misfolding and provides a paradigm for understanding the role of mutations in aminoacyl-tRNA synthetases in neurological diseases. Keywords« less

  13. Structural basis for recognition of 5'-phosphotyrosine adducts by TDP2

    SciTech Connect (OSTI)

    Shi, Ke; Kurahash, Kayo; Gao, Rui; Tsutakawa, Susan E.; Tainer, John A.; Pommier, Yves; Aihara, Hideki

    2012-12-19

    The DNA repair enzyme TDP2 resolves 5'-phosphotyrosyl-DNA adducts, and is responsible for resistance to anti-cancer drugs that target covalent topoisomerase-DNA complexes. TDP2 also participates in key signaling pathways during development and tumorigenesis, and cleaves a protein-RNA linkage during picornavirus replication. The crystal structure of zebrafish TDP2 bound to DNA reveals a deep and narrow basic groove that selectively accommodates the 5'-end of single-stranded DNA in a stretched conformation. The crystal structure of the full-length C. elegans TDP2 shows that this groove can also accommodate an acidic peptide stretch in vitro, with Glu and Asp sidechains occupying the DNA backbone phosphate binding sites. This extensive molecular mimicry suggests a potential mechanism for auto-regulation and how TDP2 may interact with phosphorylated proteins in signaling. Our study provides a framework to interrogate functions of TDP2 and develop inhibitors for chemotherapeutic and antiviral applications.

  14. Structural basis of HIV-1 capsid recognition by PF74 and CPSF6

    SciTech Connect (OSTI)

    Bhattacharya, Akash; Alam, Steven L.; Fricke, Thomas; Zadrozny, Kaneil; Sedzicki, Jaroslaw; Taylor, Alexander B.; Demeler, Borries; Pornillos, Owen; Ganser-Pornillos, Barbie K.; Diaz-Griffero, Felipe; Ivanov, Dmitri N.; Yeager, Mark

    2014-12-17

    Upon infection of susceptible cells by HIV-1, the conical capsid formed by ~250 hexamers and 12 pentamers of the CA protein is delivered to the cytoplasm. In this study, the capsid shields the RNA genome and proteins required for reverse transcription. In addition, the surface of the capsid mediates numerous host–virus interactions, which either promote infection or enable viral restriction by innate immune responses. In the intact capsid, there is an intermolecular interface between the N-terminal domain (NTD) of one subunit and the C-terminal domain (CTD) of the adjacent subunit within the same hexameric ring. The NTD–CTD interface is critical for capsid assembly, both as an architectural element of the CA hexamer and pentamer and as a mechanistic element for generating lattice curvature. Here we report biochemical experiments showing that PF-3450074 (PF74), a drug that inhibits HIV-1 infection, as well as host proteins cleavage and polyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer with at least 10-fold higher affinities compared with nonassembled CA or isolated CA domains. The crystal structure of PF74 in complex with the CA hexamer reveals that PF74 binds in a preformed pocket encompassing the NTD–CTD interface, suggesting that the principal inhibitory target of PF74 is the assembled capsid. Likewise, CPSF6 binds in the same pocket. Given that the NTD–CTD interface is a specific molecular signature of assembled hexamers in the capsid, binding of NUP153 at this site suggests that key features of capsid architecture remain intact upon delivery of the preintegration complex to the nucleus.

  15. Basis for Identification of Disposal Options for R and D for...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Used Fuel Disposition campaign (UFD) is selecting a set of geologic media for further ... to mined geologic disposal in the three media (salt, clayshale, and granitic rocks), ...

  16. Crystal Structures of mPGES-1 Inhibitor Complexes Form a Basis...

    Office of Scientific and Technical Information (OSTI)

    Srinivasan ; Backer, Ryan ; Zhang, Aiping ; Russell, Marijane ; Chang, Shawn S. ; Harvey, Anita ; Sloan, Ashley V. ; Fisher, Matthew J. 1 ; Lilly) 2 + Show Author ...

  17. Understanding Contaminant Transport Pathways at Rocky Flats - A Basis for the Remediation Strategy

    SciTech Connect (OSTI)

    Paton, Ian

    2008-01-15

    The Rocky Flats Environmental Technology Site (RFETS) is a Department of Energy facility located approximately 16 miles northwest of Denver, Colorado. Processing and fabrication of nuclear weapons components occurred at Rocky Flats from 1952 through 1989. Operations at the Site included the use of several radionuclides, including plutonium-239/240 (Pu), americium-241 (Am), and various uranium (U) isotopes, as well as several types of chlorinated solvents. The historic operations resulted in legacy contamination, including contaminated facilities, process waste lines, buried wastes and surface soil contamination. Decontamination and removal of buildings at the site was completed in late 2005, culminating more than ten years of active environmental remediation work. The Corrective Action Decision/Record of Decision was subsequently approved in 2006, signifying regulatory approval and closure of the site. The use of RFETS as a National Wildlife Refuge is scheduled to be in full operation by 2012. To develop a plan for remediating different types of radionuclide contaminants present in the RFETS environment required understanding the different environmental transport pathways for the various actinides. Developing this understanding was the primary objective of the Actinide Migration Evaluation (AME) project. Findings from the AME studies were used in the development of RFETS remediation strategies. The AME project focused on issues of actinide behavior and mobility in surface water, groundwater, air, soil and biota at RFETS. For the purposes of the AME studies, actinide elements addressed included Pu, Am, and U. The AME program, funded by DOE, brought together personnel with a broad range of relevant expertise in technical investigations. The AME advisory panel identified research investigations and approaches that could be used to solve issues related to actinide migration at the Site. An initial step of the AME was to develop a conceptual model to provide a qualitative description of the relationships among potential actinide sources and transport pathways at RFETS. One conceptual model was developed specifically for plutonium and americium, because of their similar geochemical and transport properties. A separate model was developed for uranium because of its different properties and mobility in the environment. These conceptual models were guidelines for quantitative analyses described in the RFETS Pathway Analysis Report, which used existing data from the literature as well as site-specific analyses, including field, laboratory and modeling studies to provide quantitative estimates of actinide migration in the RFETS environment. For pathways where more than one method was used to estimate offsite loads for a specific pathway, the method yielding the highest estimated off-site was used for comparison purposes. For all actinides studied, for pre-remediation conditions, air and surface water were identified to be the dominant transport mechanisms. The estimated annual airborne plutonium-239/240 load transported off site exceeded the surface water load by roughly a factor of 40. However, despite being the largest transport pathway, airborne radionuclide concentrations at the monitoring location with the highest measurements during the period studied were less than two percent of the allowable 10 milli-rem standard governing DOE facilities. Estimated actinide loads for other pathways were much less. Shallow groundwater was approximately two orders of magnitude lower, or 1/100 of the load conveyed in surface water. The estimated biological pathway load for plutonium was approximately five orders of magnitude less, or 1/100,000, of the load estimated for surface-water. The pathway analysis results were taken into consideration during subsequent remediation activities that occurred at the site. For example, when the 903 Pad area was remediated to address elevated concentrations of Pu and Am in the surface soil, portable tent structures were constructed to prevent wind and water erosion from occurring while remediation activitie

  18. Residual radioactive contamination from decommissioning: Technical basis for translating contamination levels to annual dose

    SciTech Connect (OSTI)

    Kennedy, W.E. Jr.; Peloquin, R.A. )

    1990-01-01

    This document describes the generic modeling of the total effective dose equivalent (TEDE) to an individual in a population from a unit concentration of residual radioactive contamination. Radioactive contamination inside buildings and soil contamination are considered. Unit concentration TEDE factors by radionuclide, exposure pathway, and exposure scenario are calculated. Reference radiation exposure scenarios are used to derive unit concentration TEDE factors for about 200 individual radionuclides and parent-daughter mixtures. For buildings, these unit concentration factors list the annual TEDE for volume and surface contamination situations. For soil, annual TEDE factors are presented for unit concentrations of radionuclides in soil during residential use of contaminated land and the TEDE per unit total inventory for potential use of drinking water from a ground-water source. Because of the generic treatment of potentially complex ground-water systems, the annual TEDE factors for drinking water for a given inventory may only indicate when additional site data or modeling sophistication are warranted. Descriptions are provided of the models, exposure pathways, exposure scenarios, parameter values, and assumptions used. An analysis of the potential annual TEDE resulting from reference mixtures of residual radionuclides is provided to demonstrate application of the TEDE factors. 62 refs., 5 figs., 66 tabs.

  19. Structural Basis of Selective Ubiquitination of TRF1 by SCF[superscrip...

    Office of Scientific and Technical Information (OSTI)

    Sponsoring Org: USDOE Country of Publication: United States Language: ENGLISH Subject: 36 MATERIALS SCIENCE; CRYSTAL STRUCTURE; HOMEOSTASIS; LIGASES; MUTATIONS; PROTEOLYSIS; ...

  20. Modeling of the Reactor Core Isolation Cooling Response to Beyond Design Basis Operations - Interim Report

    SciTech Connect (OSTI)

    Ross, Kyle; Cardoni, Jeffrey N.; Wilson, Chisom Shawn; Morrow, Charles; Osborn, Douglas; Gauntt, Randall O.

    2015-12-01

    Efforts are being pursued to develop and qualify a system-level model of a reactor core isolation (RCIC) steam-turbine-driven pump. The model is being developed with the intent of employing it to inform the design of experimental configurations for full-scale RCIC testing. The model is expected to be especially valuable in sizing equipment needed in the testing. An additional intent is to use the model in understanding more fully how RCIC apparently managed to operate far removed from its design envelope in the Fukushima Daiichi Unit 2 accident. RCIC modeling is proceeding along two avenues that are expected to complement each other well. The first avenue is the continued development of the system-level RCIC model that will serve in simulating a full reactor system or full experimental configuration of which a RCIC system is part. The model reasonably represents a RCIC system today, especially given design operating conditions, but lacks specifics that are likely important in representing the off-design conditions a RCIC system might experience in an emergency situation such as a loss of all electrical power. A known specific lacking in the system model, for example, is the efficiency at which a flashing slug of water (as opposed to a concentrated jet of steam) could propel the rotating drive wheel of a RCIC turbine. To address this specific, the second avenue is being pursued wherein computational fluid dynamics (CFD) analyses of such a jet are being carried out. The results of the CFD analyses will thus complement and inform the system modeling. The system modeling will, in turn, complement the CFD analysis by providing the system information needed to impose appropriate boundary conditions on the CFD simulations. The system model will be used to inform the selection of configurations and equipment best suitable of supporting planned RCIC experimental testing. Preliminary investigations with the RCIC model indicate that liquid water ingestion by the turbine decreases the developed turbine torque; the RCIC speed then slows, and thus the pump flow rate to the RPV decreases. Subsequently, RPV water level decreases due to continued boiling and the liquid fraction flowing to the RCIC decreases, thereby accelerating the RCIC and refilling the RPV. The feedback cycle then repeats itself and/or reaches a quasi-steady equilibrium condition. In other words, the water carry-over is limited by cyclic RCIC performance degradation, and hence the system becomes self-regulating. The indications achieved to date with the system model are more qualitative than quantitative. The avenues being pursued to increase the fidelity of the model are expected to add quantitative realism. The end product will be generic in the sense that the RCIC model will be incorporable within the larger reactor coolant system model of any nuclear power plant or experimental configuration.

  1. Online Monitoring Technical Basis and Analysis Framework for Emergency Diesel Generators - Interim Report for FY 2013

    SciTech Connect (OSTI)

    Binh T. Pham; Nancy J. Lybeck; Vivek Agarwal

    2012-12-01

    The Light Water Reactor Sustainability program at Idaho National Laboratory is actively conducting research to develop and demonstrate online monitoring capabilities for active components in existing nuclear power plants. Idaho National Laboratory and the Electric Power Research Institute are working jointly to implement a pilot project to apply these capabilities to emergency diesel generators and generator step-up transformers. The Electric Power Research Institute Fleet-Wide Prognostic and Health Management Software Suite will be used to implement monitoring in conjunction with utility partners: Braidwood Generating Station (owned by Exelon Corporation) for emergency diesel generators, and Shearon Harris Nuclear Generating Station (owned by Duke Energy Progress) for generator step-up transformers. This report presents monitoring techniques, fault signatures, and diagnostic and prognostic models for emergency diesel generators. Emergency diesel generators provide backup power to the nuclear power plant, allowing operation of essential equipment such as pumps in the emergency core coolant system during catastrophic events, including loss of offsite power. Technical experts from Braidwood are assisting Idaho National Laboratory and Electric Power Research Institute in identifying critical faults and defining fault signatures associated with each fault. The resulting diagnostic models will be implemented in the Fleet-Wide Prognostic and Health Management Software Suite and tested using data from Braidwood. Parallel research on generator step-up transformers was summarized in an interim report during the fourth quarter of fiscal year 2012.

  2. Links Between Flood Frequency and Annual Water Balance Behaviors: A Basis for Similarity and Regionalization

    SciTech Connect (OSTI)

    Guo, Jiali; Li, Hongyi; Leung, Lai-Yung R.; Guo, Shenglian; Liu, Pan; Sivapalan, Murugesu

    2014-03-28

    This paper presents the results of a data based comparative study of several hundred catchments across continental United States belonging to the MOPEX dataset, which systematically explored the connection between the flood frequency curve and measures of mean annual water balance. Two different measures of mean annual water balance are used: (i) a climatic aridity index, AI, which is a measure of the competition between water and energy availability at the annual scale; and, (ii) baseflow index, BFI, the ratio of slow runoff to total runoff also at the annual time scale, reflecting the role of geology, soils, topography and vegetation. The data analyses showed that the aridity index, AI, has a first order control on both the mean and Cv of annual maximum floods. While mean annual flood decreases with increasing aridity, Cv increases with increasing aridity. BFI appeared to be a second order control on the magnitude and shape of the flood frequency curve. Higher BFI, meaning more subsurface flow and less surface flow leads to a decrease of mean annual flood whereas lower BFI leads to accumulation of soil moisture and increased flood magnitudes that arise from many events acting together. The results presented in this paper provide innovative means to delineate homogeneous regions within which the flood frequency curves can be assumed to be functionally similar. At another level, understanding the connection between annual water balance and flood frequency will be another building block towards developing comprehensive understanding of catchment runoff behavior in a holistic way.

  3. The rate schedule below was approved on a final basis by the...

    Broader source: Energy.gov (indexed) [DOE]

    by the U. S. Army Corps of Engineers (Corps) as a precaution to prevent failure of the dams, Southeastern is not able to provide peaking capacity to these customers. Southeastern...

  4. Structural Basis for Molecular Discrimination by a 3',3'-cGAMP Sensing Riboswitch

    SciTech Connect (OSTI)

    Ren, Aiming; Wang, Xin  C.; Kellenberger, Colleen  A.; Rajashankar, Kanagalaghatta  R.; Jones, Roger  A.; Hammond, Ming  C.; Patel, Dinshaw  J.

    2015-04-07

    Cyclic dinucleotides are second messengers that target the adaptor STING and stimulate the innate immune response in mammals. Besides protein receptors, there are bacterial riboswitches that selectively recognize cyclic dinucleotides. We recently discovered a natural riboswitch that targets 3',3'-cGAMP, which is distinguished from the endogenous mammalian signal 2',3'-cGAMP by its backbone connectivity. Here, we report on structures of the aptamer domain of the 3',3'-cGAMP riboswitch from Geobacter in the 3',3'-cGAMP and c-di-GMP bound states. The riboswitch adopts a tuning forklike architecture with a junctional ligand-binding pocket and different orientations of the arms are correlated with the identity of the bound cyclic dinucleotide. Subsequent biochemical experiments revealed that specificity of ligand recognition can be affected by point mutations outside of the binding pocket, which has implications for both the assignment and reengineering of riboswitches in this structural class.

  5. Structural Basis for Molecular Discrimination by a 3',3'-cGAMP Sensing Riboswitch

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ren, Aiming; Wang, Xin  C.; Kellenberger, Colleen  A.; Rajashankar, Kanagalaghatta  R.; Jones, Roger  A.; Hammond, Ming  C.; Patel, Dinshaw  J.

    2015-04-07

    Cyclic dinucleotides are second messengers that target the adaptor STING and stimulate the innate immune response in mammals. Besides protein receptors, there are bacterial riboswitches that selectively recognize cyclic dinucleotides. We recently discovered a natural riboswitch that targets 3',3'-cGAMP, which is distinguished from the endogenous mammalian signal 2',3'-cGAMP by its backbone connectivity. Here, we report on structures of the aptamer domain of the 3',3'-cGAMP riboswitch from Geobacter in the 3',3'-cGAMP and c-di-GMP bound states. The riboswitch adopts a tuning forklike architecture with a junctional ligand-binding pocket and different orientations of the arms are correlated with the identity of the boundmore » cyclic dinucleotide. Subsequent biochemical experiments revealed that specificity of ligand recognition can be affected by point mutations outside of the binding pocket, which has implications for both the assignment and reengineering of riboswitches in this structural class.« less

  6. A Report to the Secretary of Energy, Beyond Design Basis Event...

    Broader source: Energy.gov (indexed) [DOE]

    ... LANL - Los Alamos National Laboratory LLNL - Lawrence Livermore National Laboratory NBL - New Brunswick Laboratory NNSS - Nevada National Security Site *ORNL - Oak Ridge ...

  7. Water Resistant Container Technical Basis Document for the TA-55 Criticality Safety Program

    SciTech Connect (OSTI)

    Smith, Paul Herrick; Teague, Jonathan Gayle

    2015-04-30

    Criticality safety at TA-55 relies on nuclear material containers that are water resistant to prevent significant amounts of water from coming into contact with fissile material in the event of a fire that causes a breach of glovevbox confinement and subsequent fire water ingress. A “water tight container” is a container that will not allow more than 50ml of water ingress when fully submerged, except when under sufficient pressure to produce structural discontinuity. There are many types of containers, welded containers, hermetically sealed containers, filtered containers, etc.

  8. LLNL Input to SNL L2 MS: Report on the Basis for Selection of Disposal Options

    SciTech Connect (OSTI)

    Sutton, M; Blink, J A; Halsey, W G

    2011-03-02

    This mid-year deliverable has two parts. The first part is a synopsis of J. Blink's interview of the former Nevada Attorney General, Frankie Sue Del Papa, which was done in preparation for the May 18-19, 2010 Legal and Regulatory Framework Workshop held in Albuquerque. The second part is a series of sections written as input for the SNL L2 Milestone M21UF033701, due March 31, 2011. Disposal of high-level radioactive waste is categorized in this review into several categories. Section II discusses alternatives to geologic disposal: space, ice-sheets, and an engineered mountain or mausoleum. Section III discusses alternative locations for mined geologic disposal: islands, coastlines, mid-continent, and saturated versus unsaturated zone. Section IV discusses geologic disposal alternatives other than emplacement in a mine: well injection, rock melt, sub-seabed, and deep boreholes in igneous or metamorphic basement rock. Finally, Secton V discusses alternative media for mined geologic disposal: basalt, tuff, granite and other igneous/metamorphic rock, alluvium, sandstone, carbonates and chalk, shale and clay, and salt.

  9. Report to the Secretary of Energy on Beyond Design Basis Event...

    Energy Savers [EERE]

    In the six months after the March 2011 Fukushima Daiichi nuclear power plant accident in Japan, the U.S. Department of Energy (DOE) took several actions to review the safety of its ...

  10. Structural basis for substrate recognition by the human N-terminal...

    Office of Scientific and Technical Information (OSTI)

    Identifier: 1236265 Resource Type: Journal Article Resource Relation: Journal Name: Gene Dev.; Journal Volume: 29; Journal Issue: 2015 Research Org: Advanced Photon Source (APS),...

  11. Structure of the murine constitutive androstane receptor complexed to androstenol: a molecular basis for inverse agonism

    SciTech Connect (OSTI)

    Shan, L.; Vincent, J.; Brunzelle, J.S.; Dussault, I.; Lin, M.; Ianculescu, I.; Sherman, M.A.; Forman, B.M.; Fernandez, E.

    2010-03-08

    The nuclear receptor CAR is a xenobiotic responsive transcription factor that plays a central role in the clearance of drugs and bilirubin while promoting cocaine and acetaminophen toxicity. In addition, CAR has established a 'reverse' paradigm of nuclear receptor action where the receptor is active in the absence of ligand and inactive when bound to inverse agonists. We now report the crystal structure of murine CAR bound to the inverse agonist androstenol. Androstenol binds within the ligand binding pocket, but unlike many nuclear receptor ligands, it makes no contacts with helix H12/AF2. The transition from constitutive to basal activity (androstenol bound) appears to be associated with a ligand-induced kink between helices H10 and H11. This disrupts the previously predicted salt bridge that locks H12 in the transcriptionally active conformation. This mechanism of inverse agonism is distinct from traditional nuclear receptor antagonists thereby offering a new approach to receptor modulation.

  12. Defining a Technical Basis for Confidence in PV Investments - A Pathway to Service Life Prediction (Presentation)

    SciTech Connect (OSTI)

    Kurtz, S.; Wohlgemuth, J.; Kempe, M.; Bosco, N.; Hacke, P.; Jordan, D.; Miller, D.

    2013-09-01

    Four levels of accelerated test standards for PV modules are described in the context of how the community can most quickly begin using these.

  13. Basis for Section 3116 Determination for Salt Waste Disposal at the Savannah River Site

    Broader source: Energy.gov [DOE]

    The Secretary of Energy is making this 3116 Determination pursuant to Section 3116 of the Ronald W. Reagan National Defense Authorization Act for Fiscal Year 2005 (NDAA) [1]. This 3116...

  14. Advancing the Physics Basis of Quiescent H-mode through Exploration of ITER Relevant Parameters

    SciTech Connect (OSTI)

    Solomon, W. M.; Burrell, K. H.; Fenstermacher, M. E.; Garofalo, A. M.; Grierson, B. A.; Loarte, A.; McKee, G. R.; Nazikian, R.; Snyder, B. P.

    2014-09-01

    Recent experiments on DIII-D have overcome a long-standing limitation in accessing quiescent H-mode (QH-mode), a high confinement state of the plasma that does not exhibit the explosive instabilities associated with edge localized modes (ELMs). In the past, QH-mode was associated with low density operation, but has now been extended to high normalized densities compatible with operation envisioned for ITER. Through the use of strong shaping, QH-mode plasmas have been maintained at high densities, both absolute (?e ? 7 1019 m3) and normalized Greenwald fraction (?e/?G > 0:7) . In these plasmas, the pedestal can evolve to very high pressures and current as the density is increased. Calculations of the pedestal height and width from the EPED model are quantitatively consistent with the experimental observed evolution with density. The comparison of the dependence of the maximum density threshold for QH-mode with plasma shape help validate the underlying theoretical peeling-ballooning models describing ELM stability. High density QH-mode operation with strong shaping has allowed stable access to a previously predicted regime of very high pedestal dubbed \\Super H-mode". In general, QH-mode is found to achieve ELM-stable operation while maintaining adequate impurity exhaust, due to the enhanced impurity transport from an edge harmonic oscillation, thought to be a saturated kink- peeling mode driven by rotation shear. In addition, the impurity confinement time is not affected by rotation, even though the energy confinement time and measured E ? B shear is observed to increase at low toroidal rotation. Together with demonstrations of high beta, high confinement and low q95 for many energy confinement times, these results suggest QH-mode as a potentially attractive operating scenario for ITER's Q=10 mission.

  15. Understanding the nature of methane emission from rice ecosystems as basis of mitigation strategies

    SciTech Connect (OSTI)

    Buendia, L.V.; Neue, H.U.; Wassmann, R.

    1996-12-31

    Methane is considered as an important Greenhouse gas and rice fields are one of the major atmospheric methane sources. The paper aims to develop sampling strategies and formulate mitigation options based on diel (day and night) and seasonal pattern of methane emission. The study was conducted in 4 countries to measure methane flux using an automatic closed chamber system. A 24-hour bihourly methane emissions were continuously obtained during the whole growing season. Daily and seasonal pattern of methane fluxes from different rice ecosystems were evaluated. Diel pattern of methane emission from irrigated rice fields, in all sites, displayed similar pattern from planting to flowering. Fluxes at 0600, 1200, and 1800 h were important components of the total diel flux. A proposed sampling frequency to accurately estimate methane emission within the growing season was designed based on the magnitude of daily flux variation. Total methane emission from different ecosystems follow the order: deepwater rice > irrigated rice > rainfed rice. Application of pig manure increased total emission by 10 times of that without manure. Green manure application increased emission by 49% of that applied only with inorganic fertilizer. Removal of floodwater at 10 DAP and 35 DAP, within a period of 4 days, inhibited production and emission of methane. The level of variation in daily methane emission and seasonal emission pattern provides useful information for accurate determination of methane fluxes. Characterization of seasonal emission pattern as to ecologies, fertilizer amendments, and water management gives an idea of where to focus mitigation strategies for sustainable rice production.

  16. Technical Basis Agreement Document for UGTA CAU 99 RM/SM

    National Nuclear Security Administration (NNSA)

    D.L. Finnegan, J.L. Thompson, C.M. Miller, P.L. Baca, L.F. Olivas, C.G. Geoffrion, D.K. Smith, W. Goishi, B.K. Esser, J.W. Meadows, N. Namboodiri, J.F. Wild. 2001. Nevada Test Site...

  17. Core thermal response and hydrogen generation of the N Reactor hydrogen mitigation design basis accident

    SciTech Connect (OSTI)

    White, M.D.; Lombardo, N.J.; Heard, F.J.; Ogden, D.M.; Quapp, W.J.

    1988-04-01

    Calculations were performed to determine core heatup, core damage, and subsequent hydrogen production of a hypothetical loss-of-cooling accident at the Department of Energy's N Reactor. The thermal transient response of the reactor core was solved using the TRUMP-BD computer program. Estimates of whole-core thermal damage and hydrogen production were made by weighting the results of multiple half-length pressure tube simulations at various power levels. The Baker-Just and Wilson parabolic rate equations for the metal-water chemical reactions modeled the key phenomena of chemical energy and hydrogen evolution. Unlimited steam was assumed available for continuous oxidation of exposed Zircaloy-2 surfaces and for uranium metal with fuel cladding beyond the failure temperature (1038 C). Intact fuel geometry was modeled. Maximum fuel temperatures (1181 C) in the cooled central regions of the core were predicted to occur one-half hour into the accident scenario. Maximum fuel temperatures of 1447 C occurred in the core GSCS-regions at the end of the 10-h transient. After 10-h 26% of the fuel inventory was predicted to have failed. Peak hydrogen evolution equaled 42 g/s, while 10-h integrated hydrogen evolution equaled 167 kg. 12 refs., 12 figs., 2 tabs.

  18. Structural basis for selective targeting of leishmanial ribosomes: Aminoglycoside derivatives as promising therapeutics

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Shalev, Moran; Rozenberg, Haim; Smolkin, Boris; Nasereddin, Abedelmajeed; Kopelyanskiy, Dmitry; Belakhov, Valery; Schrepfer, Thomas; Schacht, Jochen; Jaffe, Charles L.; Adir, Noam; et al

    2015-08-11

    Leishmaniasis comprises an array of diseases caused by pathogenic species of Leishmania, resulting in a spectrum of mild to life-threatening pathologies. Currently available therapies for leishmaniasis include a limited selection of drugs. This coupled with the rather fast emergence of parasite resistance, presents a dire public health concern. Paromomycin (PAR), a broad-spectrum aminoglycoside antibiotic, has been shown in recent years to be highly efficient in treating visceral leishmaniasis (VL)—the life-threatening form of the disease. While much focus has been given to exploration of PAR activities in bacteria, its mechanism of action in Leishmania has received relatively little scrutiny and hasmore » yet to be fully deciphered. In the present study we present an X-ray structure of PAR bound to rRNA model mimicking its leishmanial binding target, the ribosomal A-site. We evaluate PAR inhibitory actions on leishmanial growth and ribosome function, as well as effects on auditory sensory cells, by comparing several structurally related natural and synthetic aminoglycoside derivatives. The results provide insights into the structural elements important for aminoglycoside inhibitory activities and selectivity for leishmanial cytosolic ribosomes, highlighting a novel synthetic derivative, compound 3, as a prospective therapeutic candidate for the treatment of VL.« less

  19. Structural basis for selective targeting of leishmanial ribosomes: Aminoglycoside derivatives as promising therapeutics

    SciTech Connect (OSTI)

    Shalev, Moran; Rozenberg, Haim; Smolkin, Boris; Nasereddin, Abedelmajeed; Kopelyanskiy, Dmitry; Belakhov, Valery; Schrepfer, Thomas; Schacht, Jochen; Jaffe, Charles L.; Adir, Noam; Baasov, Timor

    2015-08-11

    Leishmaniasis comprises an array of diseases caused by pathogenic species of Leishmania, resulting in a spectrum of mild to life-threatening pathologies. Currently available therapies for leishmaniasis include a limited selection of drugs. This coupled with the rather fast emergence of parasite resistance, presents a dire public health concern. Paromomycin (PAR), a broad-spectrum aminoglycoside antibiotic, has been shown in recent years to be highly efficient in treating visceral leishmaniasis (VL)—the life-threatening form of the disease. While much focus has been given to exploration of PAR activities in bacteria, its mechanism of action in Leishmania has received relatively little scrutiny and has yet to be fully deciphered. In the present study we present an X-ray structure of PAR bound to rRNA model mimicking its leishmanial binding target, the ribosomal A-site. We evaluate PAR inhibitory actions on leishmanial growth and ribosome function, as well as effects on auditory sensory cells, by comparing several structurally related natural and synthetic aminoglycoside derivatives. The results provide insights into the structural elements important for aminoglycoside inhibitory activities and selectivity for leishmanial cytosolic ribosomes, highlighting a novel synthetic derivative, compound 3, as a prospective therapeutic candidate for the treatment of VL.

  20. Structural Basis of Pre-existing Immunity to the 2009 H1N1 Pandemic...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    virus. The Sa site is located on the top of the HA trimer and colored in magenta. (B) Residual differences between CA04 and selected human H1 HAs in the Sa antigenic site ...

  1. Engineering Basis Document Review for Waste Feed Delivery from Single Shell Tanks (SST)

    SciTech Connect (OSTI)

    SMITH, D.F.

    1999-10-07

    This report provides the results of a review conducted on existing operating specifications and safety requirements and provides a summary of applicable design constraints on the Single-Shell Tank (SST) System. The SST System is required to transition from the current waste storage mission to support the Tank Waste Remediation System (TWRS) waste retrieval mission described in the Tank Waste Remediation System Mission Analysis Report (Acree 1998). The SST System is also required to support the Project Hanford Management Contract (PHMC) portions of the Waste Feed Delivery (WFD) mission. In Phase 1 the SST System will be required to retrieve waste from selected SSTs (tanks 241-C-102 and 241-C-104) for transfer to the Double-Shell Tank (DST) System (tanks 241-AZ-101,241-AY-102). The SST System will include all the systems, structures and components required to safely store, retrieve, and transfer waste in support of the TWRS mission. Operational Specification Documents (OSDs) govern operation of the existing SST System components. However, the system will be highly modified to support the TWRS mission. Therefore OSD requirements may not apply to the new system's design. This document describes the review of existing SST OSDs and provides the rationale for selecting or rejecting requirements as constraints on the SST System design. The selected requirements (or design constraints) will be included in System Specification for the Single-Shell Tank System, HNF-3912(Conrads 1999).

  2. A Report to the Secretary of Energy, Beyond Design Basis Event...

    Energy Savers [EERE]

    planning and preparedness (such as evaluations of potential loss of infrastructure or severe accident impacts to multiple facilities at a site) can benefit all DOE sites. *...

  3. A micromechanical basis for partitioning the evolution of grainbridging in brittle materials

    SciTech Connect (OSTI)

    Foulk III, J.W.; Cannon, R.M.; Johnson, G.C.; Klein, P.A.; Ritchie, R.O.

    2006-10-09

    A micromechanical model is developed for grain bridging inmonolithic ceramics. Specifically, bridge formation of a single,non-equiaxed grain spanning adjacent grains is addressed. A cohesive zoneframework enables crack initiation and propagation along grainboundaries. The evolution of the bridge is investigated through avariance in both grain angle and aspect ratio. We propose that thebridging process can be partitioned into five distinct regimes ofresistance: propagate, kink, arrest, stall, and bridge. Although crackpropagation and kinking are well understood, crack arrest and subsequent"stall" have been largely overlooked. Resistance during the stall regimeexposes large volumes of microstructure to stresses well in excess of thegrain boundary strength. Bridging can occur through continued propagationor reinitiation ahead of the stalled crack tip. The driving forcerequired to reinitiate is substantially greater than the driving forcerequired to kink. In addition, the critical driving force to reinitiateis sensitive to grain aspect ratio but relatively insensitive to grainangle. The marked increase in crack resistance occurs prior to bridgeformation and provides an interpretation for the rapidly risingresistance curves which govern the strength of many brittle materials atrealistically small flaw sizes.

  4. Technical Basis for Evaluating Surface Barriers to Protect Groundwater from Deep Vadose Zone Contamination

    SciTech Connect (OSTI)

    Fayer, Michael J.; Ward, Anderson L.; Freedman, Vicky L.

    2010-02-03

    This document presents a strategy for evaluating the effectiveness of surface barriers for site-specific deep vadose zone remediation. The strategy provides a technically defensible approach to determine the depth to which a surface barrier can effectively isolate contaminants in the vadose at a specific site as a function of subsurface properties, contaminant distribution, barrier design, and infiltration control performance. The strategy also provides an assessment of additional data and information needs with respect to surface barrier performance for deep vadose zone applications. The strategy addresses the linkage between surface barriers and deep vadose zone in situ remediation activities, monitoring issues, and emerging science, technology, and regulatory objectives. In short, the report documents the existing knowledge base, identifies knowledge needs (based on data gaps), and suggests tasks whose outcomes will address those knowledge needs. More important, the report serves as a starting point to engage the regulator and stakeholder community on the viability of deploying surface barriers for deep vadose zone contamination. As that engagement unfolds, a systematic methodology can be formalized and instituted. The strategy is focused on deep vadose zone contamination and the methods needed to determine the impact to groundwater from those deep vadose zone contaminants. Processes that affect surface barrier performance, recharge in the areas surrounding the surface barrier, and the near-surface vadose zone beneath the barrier are acknowledged but are not addressed by this strategy. In addition, the collection of site-specific data on contaminant distribution and geologic structure and properties are programmatic responsibilities and are not provided by this strategy.

  5. Final Report: Molecular Basis for Microbial Adhesion and Geochemical Surface Reactions: A Study Across Scales

    SciTech Connect (OSTI)

    Dixon, David Adams

    2013-06-27

    Computational chemistry was used to help provide a molecular level description of the interactions of Gram-negative microbial membranes with subsurface materials. The goal is to develop a better understanding of the molecular processes involved in microbial metal binding, microbial attachment to mineral surfaces, and, eventually, oxidation/reduction reactions (electron transfer) that can occur at these surfaces and are mediated by the bacterial exterior surface. The project focused on the interaction of the outer microbial membrane, which is dominated by an exterior lipopolysaccharide (LPS) portion, of Pseudomonas aeruginosa with the mineral goethite and with solvated ions in the environment. This was originally a collaborative project with T.P. Straatsma and B. Lowery of the Pacific Northwest National Laboratory. The University of Alabama effort used electronic structure calculations to predict the molecular behavior of ions in solution and the behavior of the sugars which form a critical part of the LPS. The interactions of the sugars with metal ions are expected to dominate much of the microscopic structure and transport phenomena in the LPS. This work, in combination with the molecular dynamics simulations of Straatsma and the experimental electrochemistry and microscopy measurements of Lowry, both at PNNL, is providing new insights into the detailed molecular behavior of these membranes in geochemical environments. The effort at The University of Alabama has three components: solvation energies and structures of ions in solution, prediction of the acidity of the critical groups in the sugars in the LPS, and binding of metal ions to the sugar anions. An important aspect of the structure of the LPS membrane as well as ion transport in the LPS is the ability of the sugar side groups such as the carboxylic acids and the phosphates to bind positively charged ions. We are studying the acidity of the acidic side groups in order to better understand the ability of these groups to bind metal ions. We need to understand the solvation properties of the metal ions in solution and their ability to bind not only to the sugars but to proteins and to other anions. Our goal is then to be able to predict the ability of the side groups to bind metal ions. One result from the earlier molecular dynamics simulations is the exclusion of water from the inner hydrophobic part of the membrane. We thus need to investigate the binding of the cations in media with different dielectric constants.

  6. Online Monitoring Technical Basis and Analysis Framework for Large Power Transformers; Interim Report for FY 2012

    SciTech Connect (OSTI)

    Nancy J. Lybeck; Vivek Agarwal; Binh T. Pham; Heather D. Medema; Kirk Fitzgerald

    2012-09-01

    The Light Water Reactor Sustainability program at Idaho National Laboratory (INL) is actively conducting research to develop and demonstrate online monitoring (OLM) capabilities for active components in existing Nuclear Power Plants. A pilot project is currently underway to apply OLM to Generator Step-Up Transformers (GSUs) and Emergency Diesel Generators (EDGs). INL and the Electric Power Research Institute (EPRI) are working jointly to implement the pilot project. The EPRI Fleet-Wide Prognostic and Health Management (FW-PHM) Software Suite will be used to implement monitoring in conjunction with utility partners: the Shearon Harris Nuclear Generating Station (owned by Duke Energy for GSUs, and Braidwood Generating Station (owned by Exelon Corporation) for EDGs. This report presents monitoring techniques, fault signatures, and diagnostic and prognostic models for GSUs. GSUs are main transformers that are directly connected to generators, stepping up the voltage from the generator output voltage to the highest transmission voltages for supplying electricity to the transmission grid. Technical experts from Shearon Harris are assisting INL and EPRI in identifying critical faults and defining fault signatures associated with each fault. The resulting diagnostic models will be implemented in the FW-PHM Software Suite and tested using data from Shearon-Harris. Parallel research on EDGs is being conducted, and will be reported in an interim report during the first quarter of fiscal year 2013.

  7. Draft Function Allocation Framework and Preliminary Technical Basis for Advanced SMR Concepts of Operations

    SciTech Connect (OSTI)

    Jacques Hugo; John Forester; David Gertman; Jeffrey Joe; Heather Medema; Julius Persensky; April Whaley

    2013-08-01

    This report presents preliminary research results from the investigation into the development of new models and guidance for Concepts of Operations in advanced small modular reactor (AdvSMR) designs. AdvSMRs are nuclear power plants (NPPs), but unlike conventional large NPPs that are constructed on site, AdvSMRs systems and components will be fabricated in a factory and then assembled on site. AdvSMRs will also use advanced digital instrumentation and control systems, and make greater use of automation. Some AdvSMR designs also propose to be operated in a multi-unit configuration with a single central control room as a way to be more cost-competitive with existing NPPs. These differences from conventional NPPs not only pose technical and operational challenges, but they will undoubtedly also have regulatory compliance implications, especially with respect to staffing requirements and safety standards.

  8. Development of a Technical Basis and Guidance for Advanced SMR Function Allocation

    SciTech Connect (OSTI)

    Jacques Hugo; David Gertman; Jeffrey Joe; Ronal Farris; April Whaley; Heather Medema

    2013-09-01

    This report presents the results from three key activities for FY13 that influence the definition of new concepts of operations for advanced Small Modular Reactors (AdvSMR: a) the development of a framework for the analysis of the functional environmental, and structural attributes, b) the effect that new technologies and operational concepts would have on the way functions are allocated to humans or machines or combinations of the two, and c) the relationship between new concepts of operations, new function allocations, and human performance requirements.

  9. Draft Function Allocation Framework and Preliminary Technical Basis for Advanced SMR Concepts of Operations

    SciTech Connect (OSTI)

    Jacques Hugo; John Forester; David Gertman; Jeffrey Joe; Heather Medema; Julius Persensky; April Whaley

    2013-04-01

    This report presents preliminary research results from the investigation in to the development of new models and guidance for concepts of operations (ConOps) in advanced small modular reactor (aSMR) designs. In support of this objective, three important research areas were included: operating principles of multi-modular plants, functional allocation models and strategies that would affect the development of new, non-traditional concept of operations, and the requiremetns for human performance, based upon work domain analysis and current regulatory requirements. As part of the approach for this report, we outline potential functions, including the theoretical and operational foundations for the development of a new functional allocation model and the identification of specific regulatory requirements that will influence the development of future concept of operations. The report also highlights changes in research strategy prompted by confirmationof the importance of applying the work domain analysis methodology to a reference aSMR design. It is described how this methodology will enrich the findings from this phase of the project in the subsequent phases and help in identification of metrics and focused studies for the determination of human performance criteria that can be used to support the design process.

  10. The genome of Methylobacillus flagellatus, the molecular basis forobligate methylotrophy, and the polyphyletic origin ofmethylotrophy

    SciTech Connect (OSTI)

    Chistoserdova, Ludmila; Lapidus, Alla; Han, Cliff; Goodwin,Lynne; Saunders, Liz; Brettin, Tom; Tapia, Roxanne; Gilna, Paul; Lucas,Susan; Richardson, Paul M.; Lidstrom, Mary E.

    2007-01-08

    Along with methane, methanol and methylated amines representimportant biogenic atmospheric constituents, thus not only methanotrophs,but also non-methanotrophic methylotrophs play a significant role inglobal carbon cycling. The complete genome of a model obligate methanoland methylamine utilizer, Methylobacillus flagellatus (strain KT) wassequenced. The genome is represented by a single circular chromosome ofapproximately 3 Mb pairs, potentially encoding a total of 2,766 proteins.Based on genome analysis as well as the results from previous genetic andmutational analyses, methylotrophy is enabled by methanol- andmethylamine dehydrogenases, the tetrahydromethanopterin-linkedformaldehyde oxidation pathway, the assimilatory and dissimilatorybranches of the ribulose monophosphate cycle, and by formatedehydrogenases. Some of the methylotrophy genes are present in more thanone (identical or non-identical) copy. The obligate dependence on singlecarbon compounds appears to be due to the incomplete tricarboxylic acidcycle, as no genes potentially encoding alpha ketoglutarate, malate orsuccinate dehydrogenases are identifiable. The genome of M. flagellatuswas compared, in terms of methylotrophy functions, to the previouslysequenced genomes of three methylotrophs: Methylobacterium extorquens(Alphaproteobacterium, 7 Mbp), Methylibium petroleophilum(Betaproteobacterium, 4 Mbp), and Methylococcus capsulatus(Gammaproteobacterium, 3.3 Mbp). Strikingly, metabolically and/orphylogenetically, methylotrophy functions in M. flagellatus were moresimilar to the ones in M. capsulatus and M. extorquens than to the onesin the more closely related M. petroleophilum, providing the firstgenomic evidence for the polyphyletic origin of methylotrophy inBetaproteobacteria.

  11. Common-Cause Failure Treatment in Event Assessment: Basis for a Proposed New Model

    SciTech Connect (OSTI)

    Dana Kelly; Song-Hua Shen; Gary DeMoss; Kevin Coyne; Don Marksberry

    2010-06-01

    Event assessment is an application of probabilistic risk assessment in which observed equipment failures and outages are mapped into the risk model to obtain a numerical estimate of the events risk significance. In this paper, we focus on retrospective assessments to estimate the risk significance of degraded conditions such as equipment failure accompanied by a deficiency in a process such as maintenance practices. In modeling such events, the basic events in the risk model that are associated with observed failures and other off-normal situations are typically configured to be failed, while those associated with observed successes and unchallenged components are assumed capable of failing, typically with their baseline probabilities. This is referred to as the failure memory approach to event assessment. The conditioning of common-cause failure probabilities for the common cause component group associated with the observed component failure is particularly important, as it is insufficient to simply leave these probabilities at their baseline values, and doing so may result in a significant underestimate of risk significance for the event. Past work in this area has focused on the mathematics of the adjustment. In this paper, we review the Basic Parameter Model for common-cause failure, which underlies most current risk modelling, discuss the limitations of this model with respect to event assessment, and introduce a proposed new framework for common-cause failure, which uses a Bayesian network to model underlying causes of failure, and which has the potential to overcome the limitations of the Basic Parameter Model with respect to event assessment.

  12. Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast

    SciTech Connect (OSTI)

    Ren, Xuefeng; Hurley, James H.

    2011-10-28

    The ESCRT-0 and ESCRT-I complexes coordinate the clustering of ubiquitinated cargo with intralumenal budding of the endosomal membrane, two essential steps in vacuolar/lysosomal protein sorting from yeast to humans. The 1.85-{angstrom} crystal structure of interacting regions of the yeast ESCRT-0 and ESCRT-I complexes reveals that PSDP motifs of the Vps27 ESCRT-0 subunit bind to a novel electropositive N-terminal site on the UEV domain of the ESCRT-I subunit Vps23 centred on Trp16. This novel site is completely different from the C-terminal part of the human UEV domain that binds to P(S/T)AP motifs of human ESCRT-0 and HIV-1 Gag. Disruption of the novel PSDP-binding site eliminates the interaction in vitro and blocks enrichment of Vps23 in endosome-related class E compartments in yeast cells. However, this site is non-essential for sorting of the ESCRT cargo Cps1. Taken together, these results show how a conserved motif/domain pair can evolve to use strikingly different binding modes in different organisms.

  13. Structure of NKp65 Bound to its Keratinocyte Ligand Reveals Basis...

    Office of Scientific and Technical Information (OSTI)

    Recognition in Natural Killer Gene Complex Authors: Li, Y. ; Wang, Q. ; Chen, S. ; Brown, P. ; Mariuzza, R. Publication Date: 2013-07-09 OSTI Identifier: 1162936 Report ...

  14. Structural basis of GSK-3 inhibition by N-terminal phosphorylation...

    Office of Scientific and Technical Information (OSTI)

    Authors: Stamos, Jennifer L. ; Chu, Matthew Ling-Hon ; Enos, Michael D. ; Shah, Niket ; Weis, William I. 1 + Show Author Affiliations (Stanford) Publication Date: 2015-02-19 OSTI ...

  15. Molecular Basis for Enzymatic Sulfite Oxidation -- HOW THREE CONSERVED ACTIVE SITE RESIDUES SHAPE ENZYME ACTIVITY

    SciTech Connect (OSTI)

    Bailey, Susan; Rapson, Trevor; Johnson-Winters, Kayunta; Astashkin, Andrei; Enemark, John; Kappler, Ulrike

    2008-11-10

    Sulfite dehydrogenases (SDHs) catalyze the oxidation and detoxification of sulfite to sulfate, a reaction critical to all forms of life. Sulfite-oxidizing enzymes contain three conserved active site amino acids (Arg-55, His-57, and Tyr-236) that are crucial for catalytic competency. Here we have studied the kinetic and structural effects of two novel and one previously reported substitution (R55M, H57A, Y236F) in these residues on SDH catalysis. Both Arg-55 and His-57 were found to have key roles in substrate binding. An R55M substitution increased Km(sulfite)(app) by 2-3 orders of magnitude, whereas His-57 was required for maintaining a high substrate affinity at low pH when the imidazole ring is fully protonated. This effect may be mediated by interactions of His-57 with Arg-55 that stabilize the position of the Arg-55 side chain or, alternatively, may reflect changes in the protonation state of sulfite. Unlike what is seen for SDHWT and SDHY236F, the catalytic turnover rates of SDHR55M and SDHH57A are relatively insensitive to pH (~;;60 and 200 s-1, respectively). On the structural level, striking kinetic effects appeared to correlate with disorder (in SDHH57A and SDHY236F) or absence of Arg-55 (SDHR55M), suggesting that Arg-55 and the hydrogen bonding interactions it engages in are crucial for substrate binding and catalysis. The structure of SDHR55M has sulfate bound at the active site, a fact that coincides with a significant increase in the inhibitory effect of sulfate in SDHR55M. Thus, Arg-55 also appears to be involved in enabling discrimination between the substrate and product in SDH.

  16. Technical Basis for Physical Fidelity of NRC Control Room Training Simulators for Advanced Reactors

    SciTech Connect (OSTI)

    Minsk, Brian S.; Branch, Kristi M.; Bates, Edward K.; Mitchell, Mark R.; Gore, Bryan F.; Faris, Drury K.

    2009-10-09

    The objective of this study is to determine how simulator physical fidelity influences the effectiveness of training the regulatory personnel responsible for examination and oversight of operating personnel and inspection of technical systems at nuclear power reactors. It seeks to contribute to the U.S. Nuclear Regulatory Commission’s (NRC’s) understanding of the physical fidelity requirements of training simulators. The goal of the study is to provide an analytic framework, data, and analyses that inform NRC decisions about the physical fidelity requirements of the simulators it will need to train its staff for assignment at advanced reactors. These staff are expected to come from increasingly diverse educational and experiential backgrounds.

  17. Atmos. Chem. Phys., 16, 5203-5227, ...

    Office of Scientific and Technical Information (OSTI)

    ... Meteorol., 6, 129- 150, 1974. Betts, A.: Chapter 4 The Physics and Parameterization of Moist Atmospheric Convection, in: Trade Cumulus: Observations and Modeling, edited by: Smith, ...

  18. Gas venting system

    DOE Patents [OSTI]

    Khan, Amjad; Dreier, Ken Wayne; Moulthrop, Lawrence Clinton; White, Erik James

    2010-06-29

    A system to vent a moist gas stream is disclosed. The system includes an enclosure and an electrochemical cell disposed within the enclosure, the electrochemical cell productive of the moist gas stream. A first vent is in fluid communication with the electrochemical cell for venting the moist gas stream to an exterior of the enclosure, and a second vent is in fluid communication with an interior of the enclosure and in thermal communication with the first vent for discharging heated air to the exterior of the enclosure. At least a portion of the discharging heated air is for preventing freezing of the moist gas stream within the first vent.

  19. Search for: All records | DOE PAGES

    Office of Scientific and Technical Information (OSTI)

    Facility AAF Clouds with Low Optical Water Depths CLOWD ... for the moist potential temperature m(1+1.61qv), which allows consistent treatment of moisture in the ...

  20. ARM - Facility News Article

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    meters by the wedge of moist monsoon air that pushes northward from the tropical Atlantic. ... mentoring relationships among the nascent climate science community on that continent. ...