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Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


1

Studies on the activities of rumen microorganisms in vitro  

E-Print Network [OSTI]

to the functional state of the rumen. The methods used by various investigators for measuring rumen microbial activity includei (a) cellulose digestion, (b) utilization of non-protein nitrogen, (c) production of volatile fatty acids, (d) production of carbon... EXTRACTS ON THE DIGESTION OF CELLULOSE BE RUMEN MICROORGANISMS INTRODUCTION Page Review of Literature Collection of Numen Samples Processing of Rumen Samples Measurement of the Activity of Rurren Yiicroorgan~ s'". s Procedure for ~vt-, c Incubation...

Johnson, Carl Edward

1957-01-01T23:59:59.000Z

2

A laboratory study of the activities of dried rumen microorganisms  

E-Print Network [OSTI]

. 01736 gm. /ml. By all of the criteria used, (except the digestion of carboxyzethyl cellulose) the activities of dried rumen preparations were very much lover than those of fresh rumen liquid in the "artificial rumen". This may be attributed to nne... have little or no ~ ~ act1vity vith respect to cellulose digestion, non-protein nitrogen utilisation, or vitamin synthesis. The failure of the bacteria to be reactivated may bs due to one or more of the following possible causes& (s) the bacteria...

Harbers, Leniel Henry

1958-01-01T23:59:59.000Z

3

DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY  

SciTech Connect (OSTI)

Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were re-grown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. When PHPA was used alone, about 10% of the residual oil was recovered. Interfacial tension (IFT) decreased in a stepwise manner as biosurfactant concentration increased with marked reductions in IFT occurring at biosurfactant concentrations of 10 and 40 mg/l. When the biosurfactant concentration was greater than 10 mg/l, residual oil recovery linearly increased with biosurfactant concentration. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Our work shows that (1) diverse microorganisms produce biosurfactants, (2) nutrient manipulation may provide a mechanism to increase biosurfactant activity, (3) biosurfactant concentrations in excess of the critical micelle concentration recover substantial amounts of residual oil, and (4) equations that describe the effect of the biosurfactant on IFT adequately predict residual oil recovery in sandstone cores.

M.J. McInerney; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; D. Nagle

2004-05-31T23:59:59.000Z

4

Microorganisms for producing organic acids  

DOE Patents [OSTI]

Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

Pfleger, Brian Frederick; Begemann, Matthew Brett

2014-09-30T23:59:59.000Z

5

Development of Microorganisms with Improved Transport and Biosurfactant Activity for Enhanced Oil Recovery  

SciTech Connect (OSTI)

The project had three objectives: (1) to develop microbial strains with improved biosurfactant properties that use cost-effective nutrients, (2) to obtain biosurfactant strains with improved transport properties through sandstones, and (3) to determine the empirical relationship between surfactant concentration and interfacial tension and whether in situ reactions kinetics and biosurfactant concentration meets appropriate engineering design criteria. Here, we show that a lipopeptide biosurfactant produced by Bacillus mojavensis strain JF-2 mobilized substantial amounts of residual hydrocarbon from sand-packed columns and Berea sandstone cores when a viscosifying agent and a low molecular weight alcohol were present. The amount of residual hydrocarbon mobilized depended on the biosurfactant concentration. Tertiary oil recovery experiments showed that 10 to 40 mg/l of JF-2 biosurfactant in the presence of 0.1 mM 2,3-butanediol and 1 g/l of partially hydrolyzed polyacrylamide (PHPA) recovered 10-40% of residual oil from Berea sandstone cores. Even low biosurfactant concentrations (16 mg/l) mobilized substantial amounts of residual hydrocarbon (29%). The bio-surfactant lowered IFT by nearly 2 orders of magnitude compared to typical IFT values of 28-29 mN/m. Increasing the salinity increased the IFT with or without 2,3-butanediol present. The lowest interfacial tension observed was 0.1 mN/m. A mathematical model that relates oil recovery to biosurfactant concentration was modified to include the stepwise changes in IFT as biosurfactant concentrations changes. This model adequately predicted the experimentally observed changes in IFT as a function of biosurfactant concentration. Theses data show that lipopeptide biosurfactant systems may be effective in removing hydrocarbon contamination sources in soils and aquifers and for the recovery of entrapped oil from low production oil reservoirs. Diverse microorganisms were screened for biosurfactant production and anaerobic growth at elevated salt concentrations to obtain candidates most suitable for microbial oil recovery. Seventy percent of the 205 strains tested, mostly strains of Bacillus mojavensis, Bacillus subtilis, Bacillus licheniformis, and Bacillus sonorensis, produced biosurfactants aerobically and 41% of the strains had biosurfactant activity greater than Bacillus mojavensis JF-2, the current candidate for oil recovery. Biosurfactant activity varied with the percentage of the 3-hydroxy-tetradecanoate isomers in the fatty acid portion of the biosurfactant. Changing the medium composition by incorporation of different precursors of 3-hydroxy tetradecanoate increased the activity of biosurfactant. The surface tension and critical micelle concentration of 15 different, biosurfactant-producing Bacillus strains was determined individually and in combination with other biosurfactants. Some biosurfactant mixtures were found to have synergistic effect on surface tension (e.g. surface tension was lowered from 41 to 31 mN/m in some cases) while others had a synergistic effect on CMD-1 values. We compared the transport abilities of spores from three Bacillus strains using a model porous system to study spore recovery and transport. Sand-packed columns were used to select for spores or cells with the best transport abilities through brine-saturated sand. Spores of Bacillus mojavensis strains JF-2 and ROB-2 and a natural recombinant, strain C-9, transported through sand at very high efficiencies. The earliest cells/spores that emerged from the column were regrown, allowed to sporulate, and applied to a second column. This procedure greatly enhanced the transport of strain C-9. Spores with enhanced transport abilities can be easily obtained and that the preparation of inocula for use in MEOR is feasible. We conducted a push-pull test to study in-situ biosurfactant production by exogenous biosurfactant producers to aid in oil recovery from depleted reservoirs. Five wells from the same formation were used. Two wells received cells and nutrients, two wells were treated with nutrients onl

M.J. McInerney; K.E. Duncan; N. Youssef; T. Fincher; S.K. Maudgalya; M.J. Folmsbee; R. Knapp; Randy R. Simpson; N.Ravi; D. Nagle

2005-08-15T23:59:59.000Z

6

active living principles: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

theory of generalized functions. Vernaeve, Hans 2011-01-01 235 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

7

E-Print Network 3.0 - activated sludge microorganisms Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Computer Science, Texas Woman's University Collection: Mathematics 8 Advanced Wastewater Treatment Processes Summary: Treatment Plant Conventional Activated Sludge Process...

8

E-Print Network 3.0 - active living study Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

RH 21% Summary: All double rooms Community Based Living (CBLV): West College: Political Activism and Artistic... % Where will I end up? Below is a snapshot of where the...

9

Recombinant microorganisms for increased production of organic acids  

DOE Patents [OSTI]

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Yi, Jian; Kleff, Susanne; Guettler, Michael V

2013-04-30T23:59:59.000Z

10

Recombinant microorganisms for increased production of organic acids  

DOE Patents [OSTI]

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Yi, Jian (East Lansing, MI); Kleff, Susanne (East Lansing, MI); Guettler, Michael V. (Holt, MI)

2012-02-21T23:59:59.000Z

11

Bidentate Phenoxides as Ideal Activating Ligands for Living Ring-Opening Alkyne Metathesis Polymerization  

E-Print Network [OSTI]

Polymerization Danielle F. Sedbrook, Daniel W. Paley, Michael L. Steigerwald, Colin Nuckolls,*, and Felix R polymerization (ROAMP) of dibenzocyclooctynes. The reaction produces living polymers with low polydispersities of the polymerization reaction. We herein report the use of bidentate, chelating phenoxides as activating ligands

Hone, James

12

Supervised Classification of Activities of Daily Living in Health Smart Homes using SVM  

E-Print Network [OSTI]

/undressing). This is done using Support Vector Machines. We performed an experimentation with 13 young and healthy subjects) on real data. Index Terms--Support vector machines, Activities of Daily Living, Health Smart Homes, Sensor behavior modification and in Japan, Zojirushi Corporation is interested by the use of the electric water

Paris-Sud XI, Université de

13

Engineered microorganisms capable of producing target compounds under anaerobic conditions  

DOE Patents [OSTI]

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

2012-01-17T23:59:59.000Z

14

Activity-Based Prototyping of Ubicomp Applications for Long-Lived, Everyday Human Activities  

E-Print Network [OSTI]

design process, and it allowed creating realistic ubicomp application prototypes at a low cost computing (ubicomp) promises to support our everyday activities by weaving computing power into the fabric by orienting designs towards human needs. ACD, however, is largely a set of perspectives and concepts

Anderson, Richard

15

Fast-Neutron Activation of Long-Lived Nuclides in Natural Pb  

E-Print Network [OSTI]

We measured the production of the long-lived nuclides Bi-207, Pb-202, and Hg-194 in a sample of natural Pb due to high-energy neutron interactions using a neutron beam at the Los Alamos Neutron Science Center. The activated sample was counted by a HPGe detector to measure the amount of radioactive nuclides present. These nuclides are critical in understanding potential backgrounds in low background experiments utilizing large amounts of Pb shielding due to cosmogenic neutron interactions in the Pb while residing on the Earth's surface. By scaling the LANSCE neutron flux to a cosmic neutron flux, we measure the sea level cosmic ray production rates of 8.0 +/- 1.3 atoms/kg/day of Hg-194, 120 +/- 25 atoms/kg/day Pb-202, and 0.17 +/- 0.04 atoms/kg/day Bi-207.

V. E. Guiseppe; S. R. Elliott; N. E. Fields; D. Hixon

2012-09-20T23:59:59.000Z

16

E-Print Network 3.0 - activities of daily living Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Centre de mathmatiques Collection: Mathematics 55 Living Letters Introduction: First grade students practice writing the letters of the alphabet almost on a daily Summary:...

17

The Lively PartsBin--A Cloud-based Repository for Collaborative Development of Active Web Content  

E-Print Network [OSTI]

The Lively Kernel is a browser-based environment for authoring active Web content. Being a self- supporting system, it is simple and compact, yet it sup- ports rapid authoring with little need for programming] surprised and delighted many people by showing that it is possible to build a com- plete self

Weske, Mathias

18

Live-cell Imaging of Pol II Promoter Activity to Monitor Gene expression with RNA IMAGEtag reporters  

SciTech Connect (OSTI)

We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that can be expressed from a promoter of choice. For imaging, the cells are incubated with their ligands that are separately conjugated with one of the FRET pair, Cy3 and Cy5. The IMAGEtags were expressed in yeast from the GAL1, ADH1 or ACT1 promoters. Transcription from all three promoters was imaged in live cells and transcriptional increases from the GAL1 promoter were observed with time after adding galactose. Expression of the IMAGEtags did not affect cell proliferation or endogenous gene expression. Advantages of this method are that no foreign proteins are produced in the cells that could be toxic or otherwise influence the cellular response as they accumulate, the IMAGEtags are short lived and oxygen is not required to generate their signals. The IMAGEtag RNA reporter system provides a means of tracking changes in transcriptional activity in live cells and in real time.

Shin, Ilchung [Ames Laboratory; Ray, Judhajeet [Ames Laboratory; Gupta, Vinayak [Iowa State University; Ilgu, Muslum [Ames Laboratory; Beasley, Jonathan [Iowa State University; Bendickson, Lee [Ames Laboratory; Mehanovic, Samir [Molecular Express; Kraus, George A. [Iowa State University; Nilsen-Hamilton, Marit [Ames Laboratory

2014-04-20T23:59:59.000Z

19

Pesticides are used to eliminate or control unwanted or harmful insects, plants, fungi, animals, or microorganisms. Currently approximately 600 approved, active pesticide ingredients are used in almost  

E-Print Network [OSTI]

Center for Environmental Health: Conducts activities related to preparing for and responding to chemical preventing illness and death by controlling the insect vectors of diseases such as malaria, and West Nile be associated with neurologic diseases such as Parkinsons and Alzheimers. CDC ACTIVITIES Several programs

20

Organic acid-tolerant microorganisms and uses thereof for producing organic acids  

DOE Patents [OSTI]

Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

Pfleger, Brian Frederick; Begemann, Matthew Brett

2014-05-06T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


21

"A strong mind in a sane body": the role of physical activity in the lives and writings of Sophia Hawthorne, Margaret Fuller, and Kate Chopin  

E-Print Network [OSTI]

"A STRONG MIND IN A SANE BODY": THE ROLE OF PHYSICAL ACTIVITY IN THE LIVES AND WRITINGS OF SOPHIA HAWTHORNE~ MARGARET FULLER, AND KATE CHOPIN A Thesis by LEANN MILLER WILLOUGHBY Submitted to the Office of Graduate Studies of Texas ASM... University in partial fulfillment of the requirements for the degree of MASTER OF ARTS August 1992 Major Subject: English ?A STRONG MIND IN A SANE BODY? . THE ROLE OF PHYSICAL ACTIVITY IN THE LIVES AND WRITINGS OF SOPHIA HAWTHORNE, MARGARET FULLER...

Willoughby, Leann Miller

1992-01-01T23:59:59.000Z

22

E-Print Network 3.0 - active living projects Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

4 5 > >> 41 Roberta E. Rikli, C. Jessie Jones. (1997). Assessing Physical Performance in Independent Older Adults: Issues and Guidelines. Journal of Aging and Physical Activity, 5...

23

E-Print Network 3.0 - active living environment Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

activities in our on-campus smart apartment testbed. Keywords Smart environments Passive sensors... complex (e.g., food preparation, medication man- agement, telephone...

24

Organelle-Specific Activity-Based Protein Profiling in Living Cells  

SciTech Connect (OSTI)

A multimodal acidic organelle targeting activity-based probe was developed for analysis of subcellular native enzymatic activity of cells by fluorescent microscopy and mass spectrometry. A cathepsin reactive warhead was conjugated to an acidotropic amine, and a clickable alkyne for appendage of AlexaFluor 488 or biotin reporter tags. This probe accumulated in punctate vesicles surrounded by LAMP1, a lysosome marker, as observed by Structured Illumination Microscopy (SIM) in J774 mouse macrophage cells. Biotin conjugation, affinity purification, and analysis of in vivo labeled J774 by mass spectrometry showed that the probe was very selective for Cathepsins B and Z, two lysosomal cysteine proteases. Analysis of starvation induced autophagy, which is an increase in cell component catabolism involving lysosomes, showed a large increase in tagged protein number and an increase in cathepsin activity. Organelle targeting activity-based probes and subsequent analysis of resident proteins by mass spectrometry is enabled by tuning the physicochemical properties of the probe.

Wiedner, Susan D.; Anderson, Lindsey N.; Sadler, Natalie C.; Chrisler, William B.; Kodali, Vamsi K.; Smith, Richard D.; Wright, Aaron T.

2014-02-06T23:59:59.000Z

25

A combined microfluidic/dielectrophoretic microorganism concentrator  

E-Print Network [OSTI]

This thesis presents the development of a high-throughput microfluidic microorganism concentrator for pathogen detection applications. Interdigitated electrodes lining the bottom of the channel use positive dielectrophoretic ...

Gadish, Nitzan

2005-01-01T23:59:59.000Z

26

Biodegradation of Triclosan by Aerobic Microorganisms  

E-Print Network [OSTI]

and limited. To bridge this knowledge gap, this dissertation characterized cultivable triclosan-degrading microorganisms, identified uncultivable triclosan-utilizing bacteria, and elucidated triclosan biodegradation pathways. Furthermore, two treatment...

Lee, Do Gyun

2012-10-19T23:59:59.000Z

27

Emerging contaminants and microorganisms into the environment  

E-Print Network [OSTI]

Workshop Emerging contaminants and microorganisms into the environment: contamination pathways Environmental Engineering Division Surname _____________________Name____________________ Job Environmental Engineering Division (SEED) Department of Civil Engineering University of Salerno Via Ponte don

Costagliola, Gennaro

28

A microfabricated dielectrophoretic micro-organism concentrator  

E-Print Network [OSTI]

This project focuses on the development of a micro-organism concentrator. Pathogen detection, particularly MEMS based detection, is often limited by sample concentration. The proposed concentrator will interface with a ...

Muller, Rikky, 1980-

2004-01-01T23:59:59.000Z

29

Living Sustainably  

E-Print Network [OSTI]

sustainable society does something more than keep people alive; livingsustainable modes of behavior that also lead to quality in living.

Milbrath, Lester W.

1998-01-01T23:59:59.000Z

30

Nearby Outdoor Environmental Support of Older Adults' Yard Activities, Neighborhood Walking and Independent Living in the Community  

E-Print Network [OSTI]

to remain healthy and gives them the ability to access daily-life services, and thus extend their independent years at home. Nearby outdoor environments on residential sites and in the neighborhoods may influence older adults' independent living through...

Wang, Zhe

2011-02-22T23:59:59.000Z

31

Neuroticism, Social Support and Activities of Daily Living in the Health Longevity Survey of the Old in China   

E-Print Network [OSTI]

mediator. Therefore, this study examined these findings for older people in China. Based on Chinese Longitudinal Health Longevity Survey dataset, 11,147 participants living in 22 of the 31 provinces in China were interviewed in 2000 by self...

Wu, Na

2010-11-24T23:59:59.000Z

32

Results an data on the growth of the microorganisms  

SciTech Connect (OSTI)

The study of biocorrosion of aluminum and its alloy was performed under conditions of continuous fermentation of thermophilic anaerobic microorganisms of different groups. This allowed us to examine the effect of various types of metabolic reactions of reduction-oxidation proceeding at different pH and temperatures under highly reduced conditions on aluminum corrosion. Besides, the experiments were performed where the part of the standard sample was exposed under strictly anaerobic conditions with an active microbiological process, and the second half was exposed under aerobic conditions. Thus the sample was exposed in gradient of oxidized-reduced conditions.

Laurinavichius, K.S.

1995-10-09T23:59:59.000Z

33

Control and Manipulation of Pathogens with an Optical Trap for Live Cell Imaging of Intercellular Interactions  

E-Print Network [OSTI]

The application of live cell imaging allows direct visualization of the dynamic interactions between cells of the immune system. Some preliminary observations challenge long-held beliefs about immune responses to microorganisms; ...

Tam, Jenny M.

34

Electric DNA chips for determination of pathogenic microorganisms  

E-Print Network [OSTI]

Electric DNA chips for determination of pathogenic microorganisms Yanling Liu Doctoral thesis Liu (2008): Electric DNA chips for determination of pathogenic microorganisms. School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden Abstract Silicon-based electric DNA chip arrays

Enfors, Sven-Olof

35

Storing data encoded DNA in living organisms  

DOE Patents [OSTI]

Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

2006-06-06T23:59:59.000Z

36

Recent activities for ?-decay half-lives and ?-delayed neutron emission of very neutron-rich isotopes  

SciTech Connect (OSTI)

Beta-delayed neutron (?n) emitters play an important, two-fold role in the stellar nucleosynthesis of heavy elements in the 'rapid neutron-capture process' (r process). On one hand they lead to a detour of the material ?-decaying back to stability. On the other hand, the released neutrons increase the neutron-to-seed ratio, and are re-captured during the freeze-out phase and thus influence the final solar r-abundance curve. A large fraction of the isotopes inside the r-process reaction path are not yet experimentally accessible and are located in the (experimental) 'Terra Incognita'. With the next generation of fragmentation and ISOL facilities presently being built or already in operation, one of the main motivation of all projects is the investigation of these very neutron-rich isotopes. A short overview of one of the planned programs to measure ?n-emitters at the limits of the presently know isotopes, the BRIKEN campaign (Beta delayed neutron emission measurements at RIKEN) will be given. Presently, about 600 ?-delayed one-neutron emitters are accessible, but only for a third of them experimental data are available. Reaching more neutron-rich isotopes means also that multiple neutron-emission becomes the dominant decay mechanism. About 460 ?-delayed two-, three-or four-neutron emitters are identified up to now but for only 30 of them experimental data about the neutron branching ratios are available, most of them in the light mass region below A=30. The International Atomic and Energy Agency (IAEA) has identified the urgency and picked up this topic recently in a 'Coordinated Research Project' on a 'Reference Database for Beta-Delayed Neutron Emission Data'. This project will review, compile, and evaluate the existing data for neutron-branching ratios and half-lives of ?-delayed neutron emitters and help to ensure a reliable database for the future discoveries of new isotopes and help to constrain astrophysical and theoretical models.

Dillmann, Iris [TRIUMF, Vancouver BC, V6T 2A3, Canada and GSI Helmholtzzentrum für Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Abriola, Daniel [Laboratorio Tandar, Comisión Nacional de Energía Atómica, B1650KINA, San Martín, Buenos Aires (Argentina); Singh, Balraj [Department of Physics and Astronomy, McMaster University, Hamilton ON, L8S 4M1 (Canada)

2014-05-02T23:59:59.000Z

37

Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)  

SciTech Connect (OSTI)

Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

Ann M. Stock

2009-04-08T23:59:59.000Z

38

Deciphering Active Estrogen-Degrading Microorganisms in Bioreactors  

E-Print Network [OSTI]

in biological wastewater treatment processes. This dissertation investigated factors affecting estrogen biodegradation in bioreactors. Specifically, research efforts were placed on characterization of several bacterial estrogen degraders (model strains...

Roh, Hyung Keun

2010-10-12T23:59:59.000Z

39

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism  

DOE Patents [OSTI]

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

1992-01-01T23:59:59.000Z

40

Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism  

DOE Patents [OSTI]

A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

Gaddy, J.L.; Clausen, E.C.

1992-12-22T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


41

Selective microorganism concentration using a dielectrophoresis-based microfabricated device  

E-Print Network [OSTI]

Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

Pucha?a, Katarzyna Anna

2007-01-01T23:59:59.000Z

42

Microorganisms having enhanced resistance to acetate and methods of use  

SciTech Connect (OSTI)

The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

Brown, Steven D; Yang, Shihui

2014-10-21T23:59:59.000Z

43

Microorganisms having enhanced tolerance to inhibitors and stress  

DOE Patents [OSTI]

The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

Brown, Steven D.; Yang, Shihui

2014-07-29T23:59:59.000Z

44

Living a Sustainable Future  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

solve the energy crisis through biological methods, including genetically engineering algae and cyanobacteria. Create a Sustainable Future: Living Living a Sustainable Future How...

45

(Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)  

SciTech Connect (OSTI)

Hydrogenases are enzymes which catalyze reactions involving dihydrogen. They serve integral roles in a number of microbial metabolic pathways. Our research is focussed on investigations of the catalytic mechanism of the hydrogenases found in aerobic, N{sub 2}-fixing microorganisms such as Azotobacter vinelandii and the agronomically important Bradyrhizobium japonicum as well as microorganisms with similar hydrogenases. The hydrogenases isolated from these microorganisms are Ni- and Fe-containing heterodimers. Our work has focussed on three areas during the last grant period. In all cases, a central theme has been the role of inhibitors in the characteristics under investigation. In addition, a number of collaborative efforts have yielded interesting results. In metalloenzymes such as hydrogenase, inhibitors often influence the activity of the enzyme through ligand interactions with redox centers, often metals, within the enzyme. Therefore, investigations of the ability of various compounds to inhibit an enzyme's activity, as well as the mechanism of inhibition, can provide insight into the catalytic mechanism of the enzyme as well as the role of various redox centers in catalysis. We have investigated in detail four inhibitors of A. vinelandii and the results are summarized here. The influence of these inhibitors on the spectral properties of the enzyme are summarized. Electron paramagnetic resonance and ultraviolet spectra investigations are discussed. 9 figs.

Arp, D.J.

1990-01-01T23:59:59.000Z

46

Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control  

SciTech Connect (OSTI)

On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U. [Univ. of Tennessee, Knoxville, TN (United States); Burlage, R. [Oak Ridge National Lab., TN (United States). Environmental Sciences Div.

1998-11-01T23:59:59.000Z

47

FUTURE LOGISTICS LIVING LABORATORY  

E-Print Network [OSTI]

FUTURE LOGISTICS LIVING LABORATORY Delivering Innovation The Future Logistics Living Lab is a collaboration between NICTA, SAP and Fraunhofer. Australia's first Living Lab provides a platform for industry and research to work together, to investigate real-world problems and to demonstrate innovative technology

Heiser, Gernot

48

Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons  

DOE Patents [OSTI]

A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

Fliermans, Carl B. (Augusta, GA)

1989-01-01T23:59:59.000Z

49

Laboratory and Field Evidence for Long-Term Starvation Survival of Microorganisms in Subsurface Terrestrial Environments  

SciTech Connect (OSTI)

BIOGEOCHEMICAL MODELING OF GROUNDWATER FLOW AND NUTRIENT FLUX IN SUBSURFACE ENVIRONMENTS INDICATES THAT INHABITANT MICROORGANISMS EXPERIENCE SEVERE NUTRIENT LIMITATION. USING LABORATORY AND FIELD METHODS, WE HAVE BEEN TESTING STARVATION SURVIVAL IN SUBSURFACE MICROORGANISMS. IN MICROCOSM EXPERIMENTS, WE HAVE SHOWN THAT STRAINS OF TWO COMMONLY ISOLATED SUBSURFACE GENERA, ARTHROBACTER AND PSEUDOMONAS, ARE ABLE TO MAINTAIN VIABILITY IN LOW-NUTRIENT, NATURAL SUBSURFACE SEDIMENTS FOR OVER ONE YEAR. THESE NON-SPORE-FORMING BACTERIA UNDERGO RAPID INITIAL MINIATURIZATION FOLLOWED BY A STABILIZATION OF CELL SIZE. MEMBRANE LIPID PHOSPHOLIPID FATTY ACID (PLFA) PROFILES OF THE PSEUDOMONAS ARE CONSISTENT WITH ADAPTATION TO NUTRIENT STRESS; ARTHROBACTER APPARENTLY RESPONDS TO NUTRIENT DEPRIVATION WITHOUT ALTERING MEMBRANE PLFA. TO TEST SURVIVABILITY OF MICROORGANISMS OVER A GEOLOGIC TIME SCALE, WE CHARACTERIZED MICROBIAL COMMUNITIES IN A SEQUENCE OF UNSATURATED SEDIMENTS RANGING IN AGE FROM MODEM TO {gt}780,000 years. Sediments were relatively uniform silts in Eastern Washington State. Porewater ages at depth (measured by the chloride mass-balance approach) were as old as 3,600 years. Microbial abundance, biomass, and activities (measured by direct counts, culture counts, total PLFAs, and radiorespirometry) declined with sediment age. The pattern is consistent with laboratory microcosm studies of Microbial survival: rapid short-term change followed by long-term survival of a proportion of cells. Even the oldest sediments evinced a small but viable Microbial community. Microbial survival appeared to be a function of sediment age. Porewater age appeared to influence the markup of surviving communities, as indicated by PLFA profiles. Sites with different Porewater recharge rates and patterns of Pleistocene flooding had different communities.

Kieft, T.L. [Biology Dept., New Mexico Inst. of Mining and Technology, Socorro, NM (United States); Murphy, E.M. [Pacific Northwest National Lab., Richland, WA (United States); Amy, P.S.; Haldeman, D.L. [Department of Biological Sciences, University of Nevada, Las Vegas, NV (United States); Ringelberg, D. B. [Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States)]|[Oak Ridge National Lab., TN (United States)

1997-12-31T23:59:59.000Z

50

Encapsulation method for maintaining biodecontamination activity  

DOE Patents [OSTI]

A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.

Rogers, Robert D. (Idaho Falls, ID); Hamilton, Melinda A. (Idaho Falls, ID); Nelson, Lee O. (Idaho Falls, ID); Benson, Jennifer (Cockermouth, GB); Green, Martin J. (Wooton, GB); Milner, Timothy N. (Centerville, VA)

2002-01-01T23:59:59.000Z

51

Encapsulation method for maintaining biodecontamination activity  

DOE Patents [OSTI]

A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.

Rogers, Robert D.; Hamilton, Melinda A.; Nelson, Lee O.; Benson, Jennifer; Green, Martin J.; Milner, Timothy N.

2006-04-11T23:59:59.000Z

52

E-Print Network 3.0 - activated-sludge microbial community Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

(biofilm, 95 h; activated sludge... ). Rich sources of microorganisms, such as wastewater, activated ... Source: Logan, Bruce E.- Department of Civil and Environmental...

53

Activation cross-sections of long lived products of deuteron induced nuclear reactions on dysprosium up to 50 MeV  

E-Print Network [OSTI]

Activation cross-sections for production of 162m,161,155Ho,165,159,157,155Dy and 161,160,156,155Tb radionuclides in deuteron induced nuclear reactions on elemental dysprosium were measured up to 50 MeV for practical application and the test of the predictive power of nuclear reaction model codes. A stacked-foil irradiation technique and off-line gamma-ray spectrometry were used to determine the activities. No earlier cross-section data were found in the literature. The experimental data are compared with the predictions of the ALICE-D, EMPIRE-D and TALYS codes. Integral production yields were calculated from the fitted experimental data.

F. Tárkányi; F. Ditrói; S. Takács; J. Csikai; A. Hermanne; A. V. Ignatyuk

2014-02-05T23:59:59.000Z

54

Microorganisms to Speed Production of Biofuels - Energy Innovation Portal  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces andMapping theEnergyInnovationMichaelGE ResearchersIndustrialMicroorganisms

55

Effects of remediation amendments on vadose zone microorganisms  

SciTech Connect (OSTI)

Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

Miller, Hannah M.; Tilton, Fred A.

2012-08-10T23:59:59.000Z

56

FROM MATHEMATICS FOR LIVING TO LIVING FOR MATHEMATICS  

E-Print Network [OSTI]

FROM MATHEMATICS FOR LIVING TO LIVING FOR MATHEMATICS George Malaty, University of Joensuu, Finland "Life is good for only two things, discovering mathematics and teaching mathematics". Siméon Poisson (1781-1840) Mathematics for living and living for mathematics are related to the goals of mathematics

Spagnolo, Filippo

57

E-Print Network 3.0 - airborne moisture-indicating microorganisms...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

surface. In order to investigate the microorganisms present... obtained on the Marine Agar plates indicating a possible marine source for some of these airborne Source: Rainey,...

58

E-Print Network 3.0 - acidophilic microorganisms isolated Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Thermo-acidophilic Cyanidiophyceae (Cyanidiales)" Summary: AND DEBASHISH BHATTACHARYA Johnson, D.B. (1998) Biodiversity and ecology of acidophilic microorganisms. FEMS... Biodata...

59

E-Print Network 3.0 - active biopolymer network Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

in the blood flow, active swimming of microorganisms (e.g. sperm, bacteria ... Source: Frey, Erwin - Arnold Sommerfeld Center for Theoretical Physics, Ludwig-Maximilians-Univers...

60

Living SafeLy  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces and Interfaces Sample6, 2011LiisaInnovationorganization |SecurityLiving

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


61

Studies of Copper Nanoparticles Effects on Micro-organisms  

E-Print Network [OSTI]

We discuss about the antibacterial activities of copper nanoparticles on both Gram negative and Gram positive bacteria in this investigation. First time, we increase its antibacterial activities by using electrical power while on electrolysis synthesis and it is confirmed from its more antibacterial activities (For Escherichia coli bacteria). We investigate the changes of surface area to volume ratio of copper nanoparticles prepared in two different methods and its effects on antibacterial activities. We note that slight change of surface area to volume ratio results in the enhancement of its antibacterial activities.

T. Theivasanthi; M. Alagar

2011-10-05T23:59:59.000Z

62

Bioremediation of the organophosphate pesticide, coumaphos, using microorganisms immobilized in calcium-alginate gel beads  

E-Print Network [OSTI]

and diethylthiophosphate (DETP), using Ca-alginate immobilized cells was the focus of this research. Consortia of indigenous microorganisms capable of degrading chlorferon and DETP were isolated separately. Since chlorferon inhibited both chlorferon-degrading and DETP-degrading...

Ha, Jiyeon

2007-04-25T23:59:59.000Z

63

Technical Note: Evaluation of Effective Microorganisms (EM) In Solid Waste Management  

E-Print Network [OSTI]

Microorganisms (EM) In Solid Waste Management V. Sekeran C.is the treatment, disposal and/or recycling of solid wastes.Generally solid waste from a municipality consists of

Sekeran, V.; Balaji, C.; Bhagavathipushpa, T.

2005-01-01T23:59:59.000Z

64

E-Print Network 3.0 - anaerobic sediment microorganisms Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

microorganisms are also capable of transferring electrons to other metals and metalloids. Micro- bial reduction... of Fe(III) and other metals can influence the fate of metals in...

65

2004 Sensory Transduction in Microorganisms Gordon Research Conference-January 11-16, 2004  

SciTech Connect (OSTI)

Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environment is currently very active in a large number of laboratories in the US, Europe, Japan, and Israel. A wide range of eukaryotic and prokaryotic species are being studies with regard to their sensing of chemical changes, light and redox signal and intercellular signaling, leading either to changes in motile behavior, gene expression or development. It has become increasingly apparent that the mechanisms involved in development have application in higher organisms while the sensing systems in bacteria are involved in a very wide range of physiological traits, from pathogenicity, through to biofilm formation. This is an area where a wide range of state of the art tools have been used and developed over the past few decades. Approaches include behavioral studies, electro-physiology, genetics, molecular biology, structural biology, biophysics and single molecule microscopy, immunocytochemistry and molecular and mathematical modeling, all of this helped by the large number of bacterial and eukaryotic microbial genome sequences now available. The central goal of this meeting is to bring together investigators using this wide range of approaches and different systems to compare data, share ideas and approaches and seeks to understand the fundamental principles underlying these responses.

Judith Armitage Carlyle Storm

2005-01-07T23:59:59.000Z

66

Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol  

DOE Patents [OSTI]

The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

2014-01-14T23:59:59.000Z

67

Anaerobic Redox Cycling of Iron by Freshwater Sediment Microorganisms  

SciTech Connect (OSTI)

The potential for microbially-mediated anaerobic redox cycling of iron (Fe) was examined in a first-generation enrichment culture of freshwater wetland sediment microorganisms. MPN enumerations revealed the presence of significant populations of Fe(III)-reducing (ca. 108 cells mL-1) and Fe(II)-oxidizing, nitrate-reducing organisms (ca. 105 cells mL-1) in the sediment used to inoculate the enrichment cultures. Nitrate reduction commenced immediately following inoculation of acetate-containing (ca. 1 mM) medium with a small quantity (1% vol/vol) of wetland sediment, and resulted in the transient accumulation of NO2- and production of a mixture of end-products including NH4+. Fe(III) oxide (high surface area goethite) reduction took place - after NO3- was depleted and continued until all the acetate was utilized. Addition of NO3 after Fe(III) reduction ceased resulted in the immediate oxidation of Fe(II) coupled to reduction of + NO3-to NH4 . No significant NO2- accumulation was observed during nitrate-dependent Fe(II) oxidation. No Fe(II) oxidation occurred in pasteurized controls. Microbial community structure in the enrichment was monitored by DGGE analysis of PCR amplified 16s rDNA and RT-PCR amplified 16S rRNA, as well as by construction of 16S rDNA clone libraries for four different time points during the experiment. Strong similarities in dominant members of the microbial community were observed in the Fe(III) reduction and nitrate-dependent Fe(II) oxidation phases of the experiment, specifically the common presence of organisms closely related (= 95% sequence similarity) to the genera Geobacter and Dechloromonas. These results indicate that the wetland sediments contained organisms such as Geobacter sp. which are capable of both + dissimilatory Fe(III) reduction and oxidation of Fe(II) with reduction of NO3-reduction to NH4 . Our findings suggest that microbially-catalyzed nitrate-dependent Fe(II) oxidation has the potential to contribute to a dynamic anaerobic Fe redox cycle in freshwater sediments.

Weber, Karrie A.; Urrutia, Matilde M.; Churchill, Perry F.; Kukkadapu, Ravi K.; Roden, Eric E.

2006-01-01T23:59:59.000Z

68

Recovery of uranium by using new microorganisms isolated from North American uranium deposits  

SciTech Connect (OSTI)

Some attempts were made to remove uranium that may be present in refining effluents, mine tailings by using new microorganisms isolated from uranium deposits and peculiar natural environments. To screen microorganisms isolated from uranium deposits and peculiar natural environments in North America and Japan for maximal accumulation of uranium, hundreds of microorganisms were examined. Some microorganisms can accumulate about 500 mg (4.2 mEq) of uranium per gram of Microbial cells within 1 h. The uranium accumulation capacity of the cells exceeds that of commercially available chelating agents (2-3 mEq/g adsorbent). We attempted to recover uranium from uranium refining waste water by using new microorganisms. As a result, these microbial cells can recover trace amounts of uranium from uranium waste water with high efficiency. These strains also have a high accumulating ability for thorium. Thus, these new microorganisms can be used as an adsorbing agent for the removal of nuclear elements may be present in metallurgical effluents, mine tailings and other waste sources.

Sakaguchi, T.; Nakajima, A.; Tsuruta, T. [Miyazaki Medical College (Japan)

1995-12-31T23:59:59.000Z

69

Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater  

SciTech Connect (OSTI)

Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

Hall, Jacqueline; Hetrick, Mary; French, Todd; Hernandez, Rafael; Donaldson, Janet; Mondala, Andro; Holmes, William

2011-01-01T23:59:59.000Z

70

Effects of heavy metals on microorganisms application to process design  

E-Print Network [OSTI]

of Domestic Sewage. " Water Pollution Control, 1970, pp. 100-110. 3. Banerji, Shankha K. , discussion of "Toxicity Measurements in Activated Sludge. " by L. Hartmann and G, Laubenberger, Journal of the Sanitar En ineerin Division, ASCE, Vol. 95, No. SA4... in Aerobic Biolo ical Treatment of Waste Water Princi le and Practice. Oligodynamic Press, Houston, Texas, 1971, p. 130. 10. Hartmann, Ludwig, and Laubenberger, G. , "Toxicity Measure- ments in Activated Sludge. " Journal of the Sanita En i- neerin...

Heck, Robert Paul

1972-01-01T23:59:59.000Z

71

Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport  

SciTech Connect (OSTI)

Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

2013-09-28T23:59:59.000Z

72

The living publication  

SciTech Connect (OSTI)

Within the ICSTI Insights Series we offer three articles on the 'living publication' that is already available to practitioners in the important field of crystal structure determination and analysis. While the specific examples are drawn from this particular field, we invite readers to draw parallels in their own fields of interest. The first article describes the present state of the crystallographic living publication, already recognized by an ALPSP (Association of Learned and Professional Society Publishers) Award for Publishing Innovation in 2006. The second article describes the potential impact on the record of science as greater post-publication analysis becomes more common within currently accepted data deposition practices, using processed diffraction data as the starting point. The third article outlines a vision for the further improvement of crystallographic structure reports within potentially achievable enhanced data deposition practices, based upon raw (unprocessed) diffraction data. The IUCr in its Commissions and Journals has for many years emphasized the importance of publications being accompanied by data and the interpretation of the data in terms of atomic models. This has been followed as policy by numerous other journals in the field and its cognate disciplines. This practice has been well served by databases and archiving institutions such as the Protein Data Bank (PDB), the Cambridge Crystallographic Data Centre (CCDC), and the Inorganic Crystal Structure Database (ICSD). Normally the models that are archived are interpretations of the data, consisting of atomic coordinates with their displacement parameters, along with processed diffraction data from X-ray, neutron or electron diffraction studies. In our current online age, a reader can not only consult the printed word, but can display and explore the results with molecular graphics software of exceptional quality. Furthermore, the routine availability of processed diffraction data allows readers to perform direct calculations of the electron density (using X-rays and electrons as probes) or the nuclear density (using neutrons as probe) on which the molecular models are directly based. This current community practice is described in our first article. There are various ways that these data and tools can be used to further analyze the molecules that have been crystallized. Notably, once a set of results is announced via the publication, the research community can start to interact directly with the data and models. This gives the community the opportunity not only to read about the structure, but to examine it in detail, and even generate subsequent improved models. These improved models could, in principle, be archived along with the original interpretation of the data and can represent a continuously improving set of interpretations of a set of diffraction data. The models could improve both by correction of errors in the original interpretation and by the use of new representations of molecules in crystal structures that more accurately represent the contents of a crystal. These possible developments are described in our second article. A current, significant, thrust for the IUCr is whether it would be advantageous for the crystallographic community to require, rather than only encourage, the archiving of the raw (unprocessed) diffraction data images measured from a crystal, a fibre or a solution. This issue is being evaluated in detail by an IUCr Working Group (see http://forums.iucr.org). Such archived raw data would be linked to and from any associated publications. The archiving of raw diffraction data could allow as yet undeveloped processing methods to have access to the originally measured data. The debate within the community about this much larger proposed archiving effort revolves around the issue of 'cost versus benefit'. Costs can be minimized by preserving the raw data in local repositories, either at centralized synchrotron and neutron research institutes, or at research universities. Archiving raw data is also perceived as bein

Terwilliger, Thomas C. [Los Alamos National Laboratory

2012-06-04T23:59:59.000Z

73

Representation of Dormant and Active Microbial Dynamics for Ecosystem Modeling  

SciTech Connect (OSTI)

Dormancy is an essential strategy for microorganisms to cope with environmental stress. However, global ecosystem models typically ignore microbial dormancy, resulting in notable model uncertainties. To facilitate the consideration of dormancy in these large-scale models, we propose a new microbial physiology component that works for a wide range of substrate availabilities. This new model is based on microbial physiological states and the major parameters are the maximum specific growth and maintenance rates of active microbes and the ratio of dormant to active maintenance rates. A major improvement of our model over extant models is that it can explain the low active microbial fractions commonly observed in undisturbed soils. Our new model shows that the exponentially-increasing respiration from substrate-induced respiration experiments can only be used to determine the maximum specific growth rate and initial active microbial biomass, while the respiration data representing both exponentially-increasing and non-exponentially-increasing phases can robustly determine a range of key parameters including the initial total live biomass, initial active fraction, the maximum specific growth and maintenance rates, and the half-saturation constant. Our new model can be incorporated into existing ecosystem models to account for dormancy in microbially-driven processes and to provide improved estimates of microbial activities.

Wang, Gangsheng [ORNL; Mayes, Melanie [ORNL; Gu, Lianhong [ORNL; Schadt, Christopher Warren [ORNL

2014-01-01T23:59:59.000Z

74

Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment  

SciTech Connect (OSTI)

This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

Gu, April Z [Northeastern University; Wan, Kai-tak [Northeastern Univeristy

2014-09-02T23:59:59.000Z

75

E-Print Network 3.0 - activating indigenous microorganisms Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Aboriginal right.1 This Accord uses the term Indigenous to include the ... Source: Saskatchewan, University of - Department of Physics and Engineering Physics, Plasma Physics...

76

E-Print Network 3.0 - activated sludge micro-organism Sample...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Sciences and Ecology 8 Pilot Scale Study of Excess Sludge Production Reduction in Wastewater Treatment by Ozone Summary: , Dominic Frigon Department of Civil...

77

RexDalton,SanFrancisco Plans to use marine microorganisms as a  

E-Print Network [OSTI]

weaken the argument that oceanic carbon sequestration can act as a significant component in controlling weather in a region chosen for its significant impact on natural sequestration of carbon dioxide fromRexDalton,SanFrancisco Plans to use marine microorganisms as a sink for atmospheric carbon dioxide

Cai, Long

78

(SEMI-) AUTOMATIC RECOGNITION OF MICROORGANISMS IN WATER Karsten Rodenacker, Peter Gais1  

E-Print Network [OSTI]

of biocenosis in natural waters or investiga- tions on biocenosis in artificial, selective affected waters(SEMI-) AUTOMATIC RECOGNITION OF MICROORGANISMS IN WATER Karsten Rodenacker, Peter Gais1 , Uta J The structure of biocenosis is a powerful indicator for the condition of and changes in quality of the ecosystem

Rodenacker, Karsten

79

Living on Campus Living on campus at Penn State  

E-Print Network [OSTI]

. 100% Penn State Living Where to find us Penn State Altoona Housing and Food Services 3000 Ivyside Park assistance. Contact the Housing and Food Services Office to discuss the options available. Cable Television service is provided by Housing and Food Services. You may contract with the local cable TV provider

Yener, Aylin

80

Living Laboratory Application -Grounds & Landscape  

E-Print Network [OSTI]

identified in the Regents policy "Sustainability and Energy Efficiency" and related University plans (e, help to create a cohesive system of memorable spaces, optimize the use of campus land applying best practices, help to develop a campus that is environmentally sustainable, and use the campus as a living

Minnesota, University of

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


81

Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production  

SciTech Connect (OSTI)

Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

None

2010-07-15T23:59:59.000Z

82

Effects of microorganisms growth on the long-term stability of cement and bitumen  

SciTech Connect (OSTI)

Cement is used as a coating matrix for nuclear waste or as an engineered barrier of waste repositories situated in geological formations. The effect of mineral acids excreted by bacteria (Thiobacillus) or organic acids produced by fungi, on the biodegradation of cement is discussed. Organic acids are quantitatively and qualitatively determined during growth of fungi over a two-year period. Even with high pH conditions, pH of the cement {approx} 11, growth of microorganisms occurs. Biodeterioration of cement is expressed in terms of bioleaching velocity of calcium and is observed by electron microscopy. Bitumen is commonly used as a matrix for the long-term storage of radioactive wastes. Long-term biodegrability of bitumen is discussed as a function of its chemical composition and various studied microorganisms.

Libert, M.F.; Sellier, R.; Jouquet, G.; Trescinski, M.; Spor, H. [Nuclear Research Center of Cadarache, St.Paul-Lez-Durance (France)

1993-12-31T23:59:59.000Z

83

Measuring Interference Between Live Datacenter Applications  

E-Print Network [OSTI]

Measuring Interference Between Live Datacenter Applications Melanie Kambadur Columbia University in datacenters due to contention over shared hardware resources. Unfortunately, understanding interference in live datacenters is more difficult than in controlled environments or on simpler architectures. Most

Tomkins, Andrew

84

Solo living across the adult lifecourse   

E-Print Network [OSTI]

This study has created snapshots of solo living in contemporary society and developed an understanding of the social and economic factors involved in transitions in and out of solo living.

Smith, Adam; Wasoff, Fran; Jamieson, Lynn

85

Living in Germany. Studying at FAU.  

E-Print Network [OSTI]

Welcome. Living in Germany. Studying at FAU. www.fau.eu/international #12;Welcome. Living in Germany. Studying at FAU. 2 Welcome. Living in Germany. Studying at FAU. Contents Vice President`s welcome...................................................... 5 1. What you will find in this brochure .............................. 6 2. Welcome to Germany

Fiebig, Peter

86

Live Streaming with Gossip Maxime Monod  

E-Print Network [OSTI]

Live Streaming with Gossip Maxime Monod June 30, 2010 #12;Regular TV: everything HD Live streaming A source produces multimedia content n viewers (n large) broadcasting ... ... ... IP TV, Web TV, P2P TV environment ·HEAP Heterogeneous environment ·LiFT Presence of freeriders Live Streaming with Gossip 25 #12

Guerraoui, Rachid

87

Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms  

SciTech Connect (OSTI)

This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.

James C. Liao

2012-05-22T23:59:59.000Z

88

SF State | Residential Life Community Living Standards Page 1 COMMUNITY LIVING STANDARDS  

E-Print Network [OSTI]

SF State | Residential Life Community Living Standards Page 1 COMMUNITY LIVING STANDARDS A How-To Guide to Living in the SF State Residential Community 2014-2015 #12;SF State | Residential Life Community Philosophy Residential Life Staff Campus Resources One Stop Your Roommate Your Living Space

89

Live Fire Range Environmental Assessment  

SciTech Connect (OSTI)

The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

None

1993-08-01T23:59:59.000Z

90

Transcription Inhibition by Platinum DNA Cross-links in Live Mammalian Cells  

E-Print Network [OSTI]

We have investigated the processing of site-specific Pt?DNA cross-links in live mammalian cells to enhance our understanding of the mechanism of action of platinum-based anticancer drugs. The activity of platinum drugs ...

Ang, Wee Han

91

Quantitative phase microscopy for the study of electromotility in living cells  

E-Print Network [OSTI]

The electric activity of living cells is accompanied with changes in their optical and mechanical properties, which arise from the intrinsic biophysics of the cell membrane. These intrinsic changes can be used as an indicator ...

Oh, Seung-eun

2010-01-01T23:59:59.000Z

92

Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms  

SciTech Connect (OSTI)

Carbon dioxide fixation is carried out primarily through the Calvin-Benson-Bassham reductive pentose phosphate cycle, in which rubulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. The primary structure of the large subunit of form I RubisCO is well conserved; however, four distinct types, A, B, C, and D, may be distinguished. To better understand the environmental regulation of RubisCO in Lake Erie phytoplanktonic microorganisms, we have isolated total RNA and DNA from four Lake Erie sampling sites. Probes prepared from RubisCO large-subunit genes (rbcL) of the freshwater cyanobacterium Synechococcus sp. strain PCC6301 (representative of type IB) and the diatom Cylindrotheca sp. strain N1 (representative of type ID) was determined. It appeared that type ID (diatom) rbcL gene expression per gene dose decreased as the sampling sites shifted toward open water. By contrast, a similar trend was not observed for cyanobacterial (type IB) rbcL gene expression per gene dose. Thus far, a total of 21 clones of rbcL genes derived from mRNA have been obtained and completely sequenced from the Ballast Island site. For surface water samples, deduced amino acid sequences of five of six clones appeared to be representative of green algae. In contrast, six of nine sequenced rbcL clones from 10-m-deep samples were a chromophytic and rhodophytic lineages. At 5 m deep, the active CO{sub 2}-fixing planktonic organisms represented a diverse group, including organisms related to Chlorella ellipsoidea, Cylindrotheca sp. strain N1, and Olisthodiscus luteus. Although many more samplings at diverse sites must be accomplished, the discovery of distinctly different sequences of rbcL mRNA at different water depths suggests that there is a stratification of active CO{sub 2}-fixing organisms in western Lake Erie. 54 refs., 7 figs.

Xu, H.H.; Tabita, F.R. [Ohio State Univ., Columbus, OH (United States)

1996-06-01T23:59:59.000Z

93

actual living liver: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

- Liver EQA slides' 2. Electronic submissions Magee, Derek 67 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

94

abortus live vaccine: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

healthcare needs. TheVirginia Tech Virginia Tech 183 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

95

american indians living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

positive correlations with the Indian Chang, Chih-Pei 262 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

96

attenuated live vaccine: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

healthcare needs. TheVirginia Tech Virginia Tech 211 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

97

admixed population lived: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

population in urban United Nations; Nations Unies 1999-01-01 67 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

98

acomys cahirinus living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 18 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

99

arab americans living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Syria. The three countries show different Shavitt, Yuval 380 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

100

adolescent girls living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

that adolescents assign (more) Amos, Taryn 2009-01-01 139 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


101

african women living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

and Siegal 1991). Marvin P. Dawkins; Mary M. Williams 273 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

102

asthmatic children living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Texas; Kevin Purcell; Jaime Fergie; Kevin Richman; Lisa Rocha 84 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

103

Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium  

SciTech Connect (OSTI)

Protein reduction-oxidation (redox) modification is an important mechanism that allows microorganisms to sense environmental changes and initiate cellular responses. We have developed a quantitative chemical probe approach for live cell labeling of proteins that are sensitive to redox modifications. We utilize this in vivo strategy to identify 176 proteins undergoing ~5-10 fold dynamic redox change in response to nutrient limitation and subsequent replenishment in the photoautotrophic cyanobacterium, Synechococcus sp. PCC 7002. We detect redox changes in as little as 30 seconds after nutrient perturbation, and oscillations in reduction and oxidation for 60 minutes following the perturbation. Many of the proteins undergoing dynamic redox transformations participate in the major components for the production (photosystems and electron transport chains) or consumption (Calvin-Benson cycle and protein synthesis) of reductant and/or energy in photosynthetic organisms. Thus, our in vivo approach reveals new redox-susceptible proteins, in addition to validating those previously identified in vitro.

Sadler, Natalie C.; Melnicki, Matthew R.; Serres, Margrethe H.; Merkley, Eric D.; Chrisler, William B.; Hill, Eric A.; Romine, Margaret F.; Kim, Sangtae; Zink, Erika M.; Datta, Suchitra; Smith, Richard D.; Beliaev, Alex S.; Konopka, Allan; Wright, Aaron T.

2014-01-01T23:59:59.000Z

104

Extending the Operating Lives of Materials  

ScienceCinema (OSTI)

Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

Paul Jablonski

2010-09-01T23:59:59.000Z

105

Connecticut Weatherization Project Improves Lives, Receives National...  

Energy Savers [EERE]

Lives, Receives National Recognition May 6, 2014 - 12:24pm Addthis Donna Hawkins Technology Transfer Specialist, Weatherization Assistance Program Harris Walker Communications...

106

Extending the Operating Lives of Materials  

SciTech Connect (OSTI)

Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

Paul Jablonski

2010-01-26T23:59:59.000Z

107

Live with the Energy Innovation Hub Directors  

Broader source: Energy.gov [DOE]

Tune in today, Tuesday, March 6, 2012, at 2:15pm EST for a live discussion with our three Energy Innovation Hubs directors.

108

Sustainable Living Guide This sustainable living guide is the product of a class project for Geography 6250 at Memorial  

E-Print Network [OSTI]

St. John's Sustainable Living Guide #12;This sustainable living guide is the product of a class's Sustainable Living Guide. Memorial University of Newfoundland, St. John's, Newfoundland, Canada, 40 p. Any...........................................................................................................2-4 Sustainable Home Living

Oyet, Alwell

109

How sulphate-reducing microorganisms cope with stress: Lessons from systems biology  

SciTech Connect (OSTI)

Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

2011-04-01T23:59:59.000Z

110

Environmental assessment of the potential effects of aquifer thermal energy storage systems on microorganisms in groundwater  

SciTech Connect (OSTI)

The primary objective of this study was to evaluate the potential environmental effects (both adverse and beneficials) of aquifer thermal energy storage (ATES) technology pertaining to microbial communities indigenous to subsurface environments (i.e., aquifers) and the propagation, movement, and potential release of pathogenic microorganisms (specifically, Legionella) within ATES systems. Seasonal storage of thermal energy in aquifers shows great promise to reduce peak demand; reduce electric utility load problems; contribute to establishing favorable economics for district heating and cooling systems; and reduce pollution from extraction, refining, and combustion of fossil fuels. However, concerns that the widespread implementation of this technology may have adverse effects on biological systems indigeneous to aquifers, as well as help to propagate and release pathogenic organisms that enter thee environments need to be resolved. 101 refs., 2 tabs.

Hicks, R.J.; Stewart, D.L.

1988-03-01T23:59:59.000Z

111

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels  

SciTech Connect (OSTI)

The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

2009-12-02T23:59:59.000Z

112

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity  

DOE Patents [OSTI]

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

1996-02-20T23:59:59.000Z

113

Aerobic mineralization of MTBE and tert-butyl alcohol by stream-bed sediment microorganisms  

SciTech Connect (OSTI)

Microorganisms indigenous to the stream-bed sediments at two gasoline-contaminated groundwater sites demonstrated significant mineralization of the fuel oxygenates, methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA). Up to 73% of [U-{sup 14}C]-MTBE and 84% of [U-{sup 14}C]-TBA were degraded to {sup 14}CO{sub 2} under mixed aerobic/anaerobic conditions. No significant mineralization was observed under strictly anaerobic conditions. The results indicate that, under the mixed aerobic/anaerobic conditions characteristic of stream-bed sediments, microbial processes may provide a significant environmental sink for MTBE and TBA delivered to surface water bodies by contaminated groundwater or by other sources.

Bradley, P.M.; Landmeyer, J.E.; Chapelle, F.H. [Geological Survey, Columbia, SC (United States)] [Geological Survey, Columbia, SC (United States)

1999-06-01T23:59:59.000Z

114

Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity  

DOE Patents [OSTI]

This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

Premuzic, E.T.; Lin, M.

1996-02-20T23:59:59.000Z

115

s Earth and environment s Living resources  

E-Print Network [OSTI]

4 s Earth and environment s Living resources s Societies and health s Expertise and consulting of this trend is the acquisition, on a joint proposal from the Earth and Environment department and the Living phenomena so as to improve forecasting of the attendant hazards. The earth's crust: processes and natural

116

LIVE SCAN FINGERPRINTING PROCESS AND RESPONSIBILITIES  

E-Print Network [OSTI]

LIVE SCAN FINGERPRINTING PROCESS AND RESPONSIBILITIES HUMAN RESOURCES Workforce Planning | One of recruitment) to your Workforce Planning Analyst. Notify Finalists Department must notify finalists of the Live. Workforce Planning Analyst will make available at the Human Resources reception desk, the appropriate number

Eirinaki, Magdalini

117

INVEST IN YOUR BONES Living with Osteoporosis  

E-Print Network [OSTI]

INVEST IN YOUR BONES Living with Osteoporosis Leaflet 5 Living with osteoporosis can be done environment safe to avoid falls. Early detection of bone loss or osteoporosis is now possible with bone to be most effective in reducing bone loss during the five to ten years following menopause, when bone loss

118

Living Village Kioni Village, Ithaca, Greece  

E-Print Network [OSTI]

University | 2010 #12;Living | Village Kioni Village Ithaca, Greece A X A A Sustainable Ithaca ProgramLiving Village Kioni Village, Ithaca, Greece A X A A Nicholas Zaferatos Western Washington Zaferatos, Sustainable Ithaca Faculty Program Director, at nicholas.zaferatos@wwu.edu #12;Table of Contents

Zaferatos, Nicholas C.

119

1 | P a g e Living a Digital Life Camp Camp: Living a Digital Life  

E-Print Network [OSTI]

, captchas, social media, MMORPG -- this camp explores the digital world from both the user1 | P a g e Living a Digital Life Camp Camp: Living a Digital Life August 11- 15 for participants 11 - 13 years old General description: We live in a digital world, where the ground shifts faster

Seldin, Jonathan P.

120

Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization  

SciTech Connect (OSTI)

Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

2013-04-07T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


121

Thermophilic and hyperthermophilic microorganisms in 3^30C hydrothermal uids following a deep-sea volcanic eruption  

E-Print Network [OSTI]

25 (1998) 33^41 #12;£oor. Hyperthermophilic microorganisms have also recently been isolated from oil reservoirs in the North Sea, in the north slope of Alaska, and in continental reservoirs in France [5^7]. The lack of exogenous seawater in the French reservoirs, which could act as a source of hyperthermophile

Holden, James F.

122

High-Sensitivity Delayed-Coincidence Spectrometer to Search for Short-Lived Nuclear States  

E-Print Network [OSTI]

A high-sensitivity four-dimensional triple-coincidence spectrometer with two detectors is developed on the basis of an autocorrelation single-crystal scintillation time 4{\\pi} spectrometer and a semiconductor HPGe detector. The delayed-coincidence spectrometer is designed for searching for isomeric nano- and microsecond states in short-lived nuclei and for their related gamma-rays. Half-lives of short-lived states are measured in the range from 4 ns to 4 {\\mu}s. The sufficient activity of the monoisotopic source for the investigations does not exceed 0.01 {\\mu}Ci.

Morozov, V A; Norseev, Yu V; Sereeter, Z; Zlokazov, V B

2002-01-01T23:59:59.000Z

123

Evaluation of terrestrial microcosms for assessing the fate and effects of genetically engineered microorganisms on ecological processes  

SciTech Connect (OSTI)

This project evaluates and modifies the existing US Environmental Protection Agency's Office of Pesticides and Toxic Substances (EPA/OPTS) terrestrial microcosm test system and test protocols such that they can be used to determine the environmental fate and ecological hazards of genetically engineered microorganisms (GEMs). The intact soil-core microcosm represents terrestrial ecosystems, and when coupled with appropriate test protocols, such microcosms may be appropriate to define and limit risks associated with the intentional release of GEMs. The terrestrial microcosm test system was used to investigate the survival and transport of two model GEMs (Azospirillum lipoferum and Pseudomonas sp. Tn5 mutants) to various trophic levels and niches and through intact soil cores. Subsequent effects on nutrient cycling and displacement of indigenous microorganisms were evaluated. The model organisms were a diazotrophic root-colonizing bacterium (A. lipoferum) and a wheat root growth-inhibiting rhizobacterium (Pseudomonas sp.). The transposable element Tn5 was used as a genetic marker for both microorganisms in two separate experiments. The organisms were subjected to transposon mutagenesis using a broad host-range-mobilizable suicide plasmid. The transposon Tn5 conferred levels of kanamycin resistance up to 500 ..mu..g/ml (Pseudomonas sp.), which allowed for selection of the bacteria from environmental samples. The presence of Tn5 DNA in the genome of the model GEMs also allowed the use of Tn5 gene probes to confirm and enumerate the microorganisms in different samples from the microcosms. Two types of root growth-inhibiting Pseudomonas sp. Tn5 mutants were obtained and used in microcosm studies: those that lacked the ability to inhibit either wheat root growth or the growth of other microorganisms in vitro (tox/sup /minus//) and those which retained these properties (tox/sup +/). 53 refs., 7 figs., 6 tabs.

Fredrickson, J.K.; Bentjen, S.A.; Bolton, H. Jr.; Li, S.W.; Ligotke, M.W.; McFadden, K.M.; Van Voris, P.

1989-04-01T23:59:59.000Z

124

Living Rev. Solar Phys., 10, (2013), 1 http://www.livingreviews.org/lrsp-2013-1  

E-Print Network [OSTI]

Living Rev. Solar Phys., 10, (2013), 1 http://www.livingreviews.org/lrsp-2013-1 (Update of lrsp-2008-3) in solar physics L I V I N G REVIEWS A History of Solar Activity over Millennia Ilya G. Usoskin is a review of present knowledge of the long-term behavior of solar activity on a multi-millennial timescale

Usoskin, Ilya G.

125

Living With Roommates a practical guide  

E-Print Network [OSTI]

/Cleaning (personal living area) y Mail pick-up y Pet peeves y Smoking y Food (sharing/cooking) y Room temperature y Decorations y Alcohol (consumption values & practices) y Telephone/payment/usage/messages Suggested Topics

Loudon, Catherine

126

Integrated Live Help in the Information Commons  

E-Print Network [OSTI]

The University of Kansas implemented an "Ask a Librarian" email reference service in February 2002. With the arrival of our new dean, we were given a mandate to expand the existing email reference service to include a "live help" component...

Burich, Nancy J.; Devlin, Frances A.; Ludwig, Deborah

2006-06-26T23:59:59.000Z

127

The Live Room : transducing resonant architectures  

E-Print Network [OSTI]

The Live Room is a temporary site specific installation presented in building N 51, room 117 on the MIT campus on May 7, 1998 and concluded on June 10, 1998. Using small acoustic intensifying equipment which mount directly ...

Bain, Mark, 1966-

1998-01-01T23:59:59.000Z

128

campus as living laboratory institutional Sustainability Plan  

E-Print Network [OSTI]

operations campus as living laboratory diversity campus waste institutional Sustainability Plan eliminate priorities energy research 35%ofwastedivertedSTARSSilver 275 sustainability courses investment 60 accessibility faculty leadership 100+ sustainability research projects over $3.8 million in bursaries Calgary

Calgary, University of

129

Dynamical real numbers and living systems  

E-Print Network [OSTI]

Recently uncovered second derivative discontinuous solutions of the simplest linear ordinary differential equation define not only an nonstandard extension of the framework of the ordinary calculus, but also provide a dynamical representation of the ordinary real number system. Every real number can be visualized as a living cell -like structure, endowed with a definite evolutionary arrow. We discuss the relevance of this extended calculus in the study of living systems. We also present an intelligent version of the Newton's first law of motion.

Dhurjati Prasad Datta

2010-01-11T23:59:59.000Z

130

Bioengineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms  

SciTech Connect (OSTI)

In this study, the Principal Investigator, F.R. Tabita has teemed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available “unrestrained” organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.

Tabita, F. Robert [The Ohio State University] [The Ohio State University

2013-07-30T23:59:59.000Z

131

A study of the localized humoral immune response to implicated microorganisms in juvenile periodontitis  

SciTech Connect (OSTI)

A study was undertaken using an in vitro explant culture system to determine the presence of immunoglobulins (IgG, IgA, and IgM) in the supernatant fluids (SF) of disease gingival tissue explant cultures. Studies were also undertaken to determine if the de novo biosynthesis of {sup 14}C-immunoglobulins could be observed in the explant cultures of diseased tissues from juvenile periodontitis (JP) patients. Radiolabeled proteins were detected in the SF and immunodiffusion studies using goat antihuman gamma, alpha or mu chain serum revealed the presence of IgG and IgA but no IgM present in the SF of the JP gingival tissue explant cultures. Immunodiffusion studies using goat anti-human gamma chain serum with Staph protein A isolated IgG fractions of the SF, followed by autoradiography of the IgG precipitation lines demonstrated the biosynthesis of IgG by the JP gingival tissue explant cultures. The serological studies suggested that local immune response in JP was to a polymicrobic infection. The SF of JP showed significantly higher levels of antibody reactivity to B. intermedius, C. ochracea, E. nodatum and P. micros as compared to healthy tissues. The local antibody response to the microorganisms tested differed from that observed in the sera of the patients.

Hall, E.R.

1988-01-01T23:59:59.000Z

132

High Resolution 1H NMR Spectroscopy of Metabolically Active Microorganisms Using Non-Destructive Magic Angle Spinning  

SciTech Connect (OSTI)

It is shown that the resolution of the 1H NMR metabolite spectra of densely packed microbial cells or biofilms attached to solid surfaces can be significantly enhanced with the so-called PASS R.F pulse sequence combined with slow (40-100 Hz) magic angle spinning of the sample. Hence this technique can be used to study biofilm metabolism under environmentally relevant conditions in a minimally invasive way.

Hu, Jian Z.; Wind, Robert A.; Mclean, Jeffrey S.; Gorby, Yuri A.; Resch, Charles T.; Fredrickson, Jim K.

2004-12-01T23:59:59.000Z

133

In Situ Live Cell Sensing of Multiple Nucleotides Exploiting...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNARNA Aptamers and Graphene Oxide Nanosheets. In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNARNA...

134

An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms  

SciTech Connect (OSTI)

We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

2013-02-01T23:59:59.000Z

135

Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report  

SciTech Connect (OSTI)

As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

Elmore, B.B.

1993-08-01T23:59:59.000Z

136

Enabling Long-Lived Sensor Networks Through Solar Energy Harvesting  

E-Print Network [OSTI]

Long - Lived Sensor Networks through Solar Energy Harvestingsolar energy harvesting and storage device for sensor

Jason Hsu; Sadaf Zahedi; Jonathan Friedman; Aman Kansal; Vijay Raghunathan; Mani Srivastava

2005-01-01T23:59:59.000Z

137

Live Webinar on Better Buildings Challenge: Public-Sector Update  

Broader source: Energy.gov [DOE]

The Energy Department will present a live webinar titled "Better Buildings Challenge: Public-Sector Update."

138

Live Streaming with Gossip Maxime Monod  

E-Print Network [OSTI]

;2 TV ordinaire: tout en HD Live streaming Une source produit du contenu multimédia (un flux) n clients (n large) broadcasting diffusion ... ... ... IP TV, Web TV, P2P TV, ... vs 192K requêtes/jour 78K que le premier #12;·Gossip++Environment contraint ·HEAPEnvironnement hétérogène ·LiFT

Guerraoui, Rachid

139

Quality health plans & benefits Healthier living  

E-Print Network [OSTI]

to the Affordable Care Act, also known as the health care reform law, will comply with requirements for WomenQuality health plans & benefits Healthier living Financial well-being Intelligent solutions 05's Preventive Health Services. This means that for women with reproductive capacity, certain women

140

living on campus 2013 | 2014 hello & welcome  

E-Print Network [OSTI]

living on campus 2013 | 2014 #12;hello & welcome Welcome to your new home for the next year further information. Please look out for posters with the details. hello & welcome 02 warden 04 internet 16 the rules 18 faqs 22 What should I do now? 1 Knock on your neighbour's door to say hello

Painter, Kevin

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


141

Revised July 2013 ANNUAL LIVING EXPENSES*  

E-Print Network [OSTI]

ESTIMATED UNDERGRADUATE COST** TOTAL: (includes Fall & Spring semesters only) $38,668.00 GLOBAL PATHWAYS Insurance: $ 1,740.00 Other Expenses: $ 5,166.00 Annual Living Expenses Total: $18,508.00 NON) $19,885.00 Matriculation Fee: (one time charge only) $125.00 Tuition & Fees Total: $20,160.00 TOTAL

Tipple, Brett

142

living and learning Department of Residential Life  

E-Print Network [OSTI]

living and learning Accessible housing at Mizzou Department of Residential Life University is important to us. The Department of Residential Life at MU is committed to providing and improving accessible spaces for students with disabilities. Residential Life will help provide appropriate housing

Missouri-Columbia, University of

143

Discordance between living and death assemblages as evidence for anthropogenic ecological change  

E-Print Network [OSTI]

'' in settings of documented anthropogenic eutrophication (AE) than in areas where AE and other human impacts with eutrophication (anomalous abundance of seagrass-dwellers and/or scarcity of organic-loving species in the death. ecological baseline eutrophication marine communities paleoecology Human activities affect living systems

Boyce, C. Kevin

144

relatively high population density (much of it living inland) and small  

E-Print Network [OSTI]

relatively high population density (much of it living inland) and small rivers this is an important of filters such as activated carbon. The main problem is one of scale. A city such as Northampton, UK energy intensive drinking water purification technology in such a case would be an expensive undertaking

Darton, Richard

145

Fission barriers and half-lives  

SciTech Connect (OSTI)

We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

Moeller, P.; Nix, J.R.; Swiatecki, W.J.

1989-01-01T23:59:59.000Z

146

UNDERSTANDING QUALITY OF LIFE IN ASSISTED LIVING  

E-Print Network [OSTI]

UNDERSTANDING QUALITY OF LIFE IN ASSISTED LIVING: INFLUENCE OF THE BUILT AND ORGANIZATIONAL- institutional environment who do not need 24/7 nursing care Focus on quality of life (QoL) 194 registered ALs 6.2 % % Chinese 35.1 51.8 55.6 % South Asian 24.5 26.1 23.5 % Filipino 7.1 5.6 5.7 Total 66.7 83.5 84.9 #12;Our

147

Living Rev. Solar Phys., 5, (2008), 3 http://www.livingreviews.org/lrsp-2008-3 in solar physics  

E-Print Network [OSTI]

Living Rev. Solar Phys., 5, (2008), 3 http://www.livingreviews.org/lrsp-2008-3 in solar physics L I V I N G REVIEWS A History of Solar Activity over Millennia Ilya G. Usoskin Sodankyl¨a Geophysical here is a review of present knowledge of the long-term behavior of solar activity on a multi

Usoskin, Ilya G.

148

Solar Decathlon Team Leading the Way Toward Sustainable Living...  

Broader source: Energy.gov (indexed) [DOE]

Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters August 18,...

149

Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte  

E-Print Network [OSTI]

Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University Oregon Transportation Summit Sept 10, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money Official transportation data archive for the Portland-Vancouver metropolitan region

Bertini, Robert L.

150

Acting Biomass Program Manager Dr. Valerie Reed to Host Live...  

Broader source: Energy.gov (indexed) [DOE]

Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels...

151

LIVE from the White House Science Fair | Department of Energy  

Broader source: Energy.gov (indexed) [DOE]

LIVE from the White House Science Fair LIVE from the White House Science Fair October 18, 2010 - 11:31am Addthis John Schueler John Schueler Former New Media Specialist, Office of...

152

Search for Short Lived Particles in High Multiplicity Environment  

E-Print Network [OSTI]

A method of statistical selection of short lived particles in high multiplicity nucleus-nucleus collisions is discussed.

Marek Gazdzicki; Waldemar Retyk; Jan Pluta

1999-04-14T23:59:59.000Z

153

Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte  

E-Print Network [OSTI]

Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University June 23, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money 1,400,000 urban cities 3 counties 1 region #12;Intelligent Transportation Systems: Saving Lives, Time and Money Why Now

Bertini, Robert L.

154

Software Product Line Engineering for Long-lived, Sustainable Systems  

E-Print Network [OSTI]

Software Product Line Engineering for Long-lived, Sustainable Systems Robyn Lutz1,2, David Weiss1 system, long-lived system, variability, commonality/variability analysis. 1 Introduction Sustainable and requirements, distinguishes sustainable systems from legacy systems. Accordingly we use the term long-lived

Lutz, Robyn R.

155

SEMINOLES CITYINTHE YOUR GUIDE TO TALLAHASSEE & OFF-CAMPUS LIVING  

E-Print Network [OSTI]

roommate and neighbor relationships, and develop a sustainable style of living while in TallahasseeSEMINOLES CITYINTHE YOUR GUIDE TO TALLAHASSEE & OFF-CAMPUS LIVING 2013 #12;#12;Seminole Creed and living in the University and beyond. Freedom of Speech and Inquiry I will support academic freedom

McQuade, D. Tyler

156

NUMERICAL MODELLING OF MICROORGANISMS DISPERSION IN URBAN AREA: APPLICATION TO LEGIONELLA.  

E-Print Network [OSTI]

with transmission of an infectious agent from cooling towers (CT). During the episode that occured in Pas to contaminated cooling towers system may occur over distance larger than 10km. In addition, most cooling towers dispersion from a virtual cooling tower at the same location. The biological model has been activated

Paris-Sud XI, Université de

157

Proton radioactivity half lives with Skyrme interactions  

E-Print Network [OSTI]

The potential barrier impeding the spontaneous emission of protons in the proton radioactive nuclei is calculated as the sum of nuclear, Coulomb and centrifugal contributions. The nuclear part of the proton-nucleus interaction potential is obtained in the energy density formalism using Skyrme effective interaction that results into a simple algebraic expression. The half-lives of the proton emitters are calculated for the different Skyrme sets within the improved WKB framework. The results are found to be in reasonable agreement with the earlier results obtained for more complicated calculations involving finite range interactions.

T. R. Routray; Abhishek Mishra; S. K. Tripathy; B. Behera; D. N. Basu

2012-05-31T23:59:59.000Z

158

LiveFuels Inc | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 No revision hasInformation Earth's Heat JumpInc Place:KeystoneSolarListLiveFuels Inc Jump to: navigation, search

159

Live pathogens: rapid detection technique developed  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-SeriesFlickrinformationPostdocsCenterCentera A B C D E F G H ILive CellLive

160

25 | P a g e Activity 5  

E-Print Network [OSTI]

· Improved sustainable living and a reduced ecological impact on the environment Background: 30 tips in 30 days will help you incorporate sustainable living practices into your everyday life. Set a personal 25 | P a g e Activity 5 30 Tips in 30 Days Goals: · Motivate sustainable action

Brownstone, Rob

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


161

YOLO COUNTY / The Next Napa? / Living in Yolo is like living in Provence, without the three-hour lunches  

E-Print Network [OSTI]

YOLO COUNTY / The Next Napa? / Living in Yolo is like living in Provence, without the three of us who live in Yolo County have had to define ourselves gastronomically with descriptions such as acquaintances as the hinterlands of culinary civilization, even though Yolo has been quietly fueling the Bay

Kowalczykowski, Stephen C.

162

Evaluation of terrestrial microcosms for detection, fate, and survival analysis of genetically engineered microorganisms and their recombinant genetic material  

SciTech Connect (OSTI)

The research included in this document represents the current scientific information available regarding the applicability of terrestrial microcosms and related methodologies for evaluating detection methods and the fate and survival of microorganisms in the environment. The three terrestrial microcosms described in this document were used to evaluate the survival and fate of recombinant bacteria in soils and in association with plant surfaces and insects and their transport through soil with percolating water and root systems, and to test new methods and procedures to improve detection and enumeration of bacteria in soil. Simple (potting soil composed of peat mix and perlite, lacking environmental control and monitoring) and complex microcosms (agricultural soil with partial control and monitoring of environmental conditions) were demonstrated to be useful tools for preliminary assessments of microbial viability in terrestrial ecosystems. These studies evaluated the survival patterns of Enterobacter cloacae (pBR322) in soil and on plant surfaces and the ingestion of this same microorganism by cutworms and survival in the foregut and frass. The Versacore microcosm design was used to monitor the fate and competitiveness of genetically engineered bacteria in soil. Both selective media and gene probes were used successfully to follow the fate of two recombinant Pseudomonas sp. introduced into Versacore microcosms. Intact soil-core microcosms were employed to evaluate the fate and transport of genetically altered Azospirillum sp. and Pseudomonas sp. in soil and the plant rhizosphere. The usefulness of these various microcosms as a tool for risk assessment is underscored by the ease in obtaining soil from a proposed field release site to evaluate subsequent GEM fate and survival.

Fredrickson, J.K.; Seidler, R.J.

1989-02-01T23:59:59.000Z

163

Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions  

SciTech Connect (OSTI)

The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

Reed, Donald Timothy [Los Alamos National Laboratory; Borkowski, Marian [Los Alamos National Laboratory; Lucchini, Jean - Francois [Los Alamos National Laboratory; Ams, David [Los Alamos National Laboratory; Richmann, M. K. [Los Alamos National Laboratory; Khaing, H. [Los Alamos National Laboratory; Swanson, J. S. [Los Alamos National Laboratory

2010-12-10T23:59:59.000Z

164

Living and Working in the Freezer  

SciTech Connect (OSTI)

Very little data of any kind exists from the early spring in the Arctic. The reason? It's extremely cold and that makes it difficult to survive, let alone conduct science. From March through the end of April, 2011, scientists from around the world braved temperatures of -48?C in the high Canadian Arctic in the name of science. At the Catlin Arctic Survey's floating 'Ice Base' off Ellef Ringnes Island, Dr. Victoria Hill was investigating how organic material in fresh water near the surface of the ocean may be trapping heat from the sun, causing the upper ocean layers to warm. This is a very new area of research and this mechanism represents a key uncertainty in accurate modeling of ice thickness and upper ocean heat content. In this presentation Dr. Hill will talk about living and working at the ice base and discuss preliminary data from the expedition.

Hill, Victoria (Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity) [Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity

2012-02-07T23:59:59.000Z

165

Living anionic polymerization using a microfluidic reactor  

SciTech Connect (OSTI)

Living anionic polymerizations were conducted within aluminum-polyimide microfluidic devices. Polymerizations of styrene in cyclohexane were carried out at various conditions, including elevated temperature (60 °C) and high monomer concentration (42%, by volume). The reactions were safely maintained at a controlled temperature at all points in the reactor. Conducting these reactions in a batch reactor results in uncontrolled heat generation with potentially dangerous rises in pressure. Moreover, the microfluidic nature of these devices allows for flexible 2D designing of the flow channel. Four flow designs were examined (straight, periodically pinched, obtuse zigzag, and acute zigzag channels). The ability to use the channel pattern to increase the level of mixing throughout the reactor was evaluated. When moderately high molecular mass polymers with increased viscosity were made, the patterned channels produced polymers with narrower PDI, indicating that passive mixing arising from the channel design is improving the reaction conditions.

Iida, Kazunori; Chastek, Thomas Q.; Beers, Kathryn L.; Cavicchi, Kevin A.; Chun, Jaehun; Fasolka, Michael J.

2009-02-01T23:59:59.000Z

166

Noncharacteristic half-lives in radioactive decay  

SciTech Connect (OSTI)

Half-lives of radionuclides span more than 50 orders of magnitude. We characterize the probability distribution of this broad-range data set at the same time that we explore a method for fitting power laws and testing goodness-of-fit. It is found that the procedure proposed recently by Clauset et al.[SIAM Rev. 51, 661 (2009)] does not perform well as it rejects the power-law hypothesis even for power-law synthetic data. In contrast, we establish the existence of a power-law exponent with a value around 1.1 for the half-life density, which can be explained by the sharp relationship between decay rate and released energy, for different disintegration types. For the case of alpha emission, this relationship constitutes an original mechanism of power-law generation.

Corral, Alvaro; Font, Francesc [Centre de Recerca Matematica, Edifici Cc, Campus Bellaterra, E-08193 Barcelona (Spain); Camacho, Juan [Grup FESTA, Facultat de Ciencies, Universitat Autonoma de Barcelona, E-08193 Barcelona (Spain)

2011-06-15T23:59:59.000Z

167

(Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)  

SciTech Connect (OSTI)

The results of this DOE-sponsored project have contributed to our understanding of the catalytic mechanism of A. vinelandii hydrogenase. A group of inhibitors have been characterized. These provide information about the different types of redox clusters involved in catalysis and the roles of each. One group has already used acetylene in a study of three desulfovibrian hydrogenases and shown that only the NiFe hydrogenases are inhibited. We have characterized a number of spectral properties of A. vinelandii hydrogenase. The EPR signals associated with this hydrogenase in the reduced state are reminiscent of other NiFe dimeric hydrogenases such as A. eutrophus, but distinctly difference from others such as D. gigas and Chromatium vinosum. Thus, while the NiFe dimeric hydrogenases are now recognized as a large group of similar enzymes, there are differences in the spectral and catalytic properties which are not explained by their similar redox inventories, identical subunit structures, immunological cross reactivity and conserved sequences. The inhibitors we have characterized are also proving of value in the spectral characterizations. Surprisingly, we only see a significant EP signal attributable to Ni after the enzyme has been inactivated with O{sub 2} and then reduced (though not reactivated). No spectral perterbations (EPR or UV-V is) of active enzyme can be attributed to binding of H{sub 2}, even though H{sub 2} clearly binds to this form of the enzyme. Acetylene, which does not substantially perterb the EPR signal of active hydrogenase, does result in a new absorption envelope in the UV-V is spectrum. Overall, the results of this project have revealed the complex interactions of the redox clusters in catalysis through studies of inhibitor mechanisms and spectral properties. 14 refs., 9 figs.

Arp, D.J.

1991-01-01T23:59:59.000Z

168

Slow urban living apartments : transformation of five story walk-up apartments in Seoul  

E-Print Network [OSTI]

Experiential living is the new trend for future living. Whether it is through living in micro spaces, flexible units, mixed-use developments, practicing urban farming, or sharing lifestyles, these different trends of living ...

Lim, Yihyun

2014-01-01T23:59:59.000Z

169

Protein folding in crowded environments and living cells.  

E-Print Network [OSTI]

??Biomolecular dynamics and stability are predominantly investigated in vitro, and extrapolated to explain function in the living cell. In this thesis, we attempt to bridge… (more)

Dhar, Apratim

2011-01-01T23:59:59.000Z

170

Spontaneous fission half-lives for ground state nuclides  

SciTech Connect (OSTI)

Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

1993-08-01T23:59:59.000Z

171

Protein folding and diffusion: from in vitro to live cells.  

E-Print Network [OSTI]

??Protein folding landscapes and protein-protein interaction landscapes are subject to modulation by many factors inside living cells: crowding, electrostatics, hydrophobic interactions, and even hydrodynamic phenomena.… (more)

Guo, Minghao

2014-01-01T23:59:59.000Z

172

Mass Spectral Molecular Networking of Living Microbial Colonies...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

of Living Microbial Colonies. Abstract: Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a "holy grail" in...

173

asian communities living: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Colleges within large Universities Barrash, Warren 7 University Housing Designing Sustainable Communities for Living and Learning Biology and Medicine Websites Summary: The...

174

Brownsville Public Utilities Board- Green Living Residential Rebate Program  

Broader source: Energy.gov [DOE]

Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

175

Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms  

DOE Patents [OSTI]

In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.

Seibert, Michael (Lakewood, CO); Makarova, Valeriya (Golden, CO); Tsygankov, Anatoly A. (Pushchino, RU); Rubin, Andrew B. (Moscow, RU)

2007-06-12T23:59:59.000Z

176

Physiologically anaerobic microorganisms of the deep subsurface. Final performance report, June 1, 1990--August 31, 1993  

SciTech Connect (OSTI)

Anaerobic bacteria were isolated from deep subsurface sediment samples taken at study sites in Idaho (INEL) and Washington (HR) by culturing on dilute and concentrated medium. Morphologically distinct colonies were purified, and their responses to 21 selected physiological tests were determined. Although the number of isolates was small (18 INEL, 27 HR) some general patterns could be determined. Most strains could utilize all the carbon sources, however the glycerol and melizitose utilization was positive for 50% or less of the HR isolates. Catalase activity (27.78% at INEL, 74.07% at HR) and tryptophan metabolism (11.12% at INEL, 40.74% at HR) were significantly different between the two study sites. MPN and viable counts indicate that sediments near the water table yield the greatest numbers of anaerobes. Deeper sediments also appear to be more selective with the greatest number of viable counts on low-nutrient mediums. Likewise, only strictly obligate anaerobes were found in the deepest sediment samples. Selective media indicated the presence of methanogens, acetogens, and sulfate reducers at only the HR site.

Stevens, S.E. Jr.; Chung, K.T.

1993-10-01T23:59:59.000Z

177

He-jet system to study short-lived fission-product nuclei at LAMPF  

SciTech Connect (OSTI)

Experiments have been performed at LAMPF which demonstrate the feasibility of utilizing a He-jet system to transport fission products to an on-line mass separator from a target chamber mounted in the 800-MeV, >600..mu..A main proton beam. Activities of essentially all elements produced are transported with about 60% absolute efficiency, both for fission reactions and spallation reactions. Transport times are short enough to allow study of activities with half-lives as short as 300 ms. Technical features and scientific possibilities of the system are presented.

Talbert, W.L. Jr.; Bunker, M.E.; Starner, J.W.

1983-01-01T23:59:59.000Z

178

Annual report and summaries of FY 1994 activities  

SciTech Connect (OSTI)

The Energy Biosciences program was initiated in 1979 for the purpose of fulfilling the need of basic information about plants and microorganisms relating to varied energy matters. The program generates basic information that contributes significantly to future technologies involving alternate fuel generation, petroleum replacements, sustained industrial activities along with means of improving environmental conditions. The Energy Biosciences program during Fiscal Year 1994 received 139 new research applications following the screening of numerous preapplications. Of the applications received, 21 projects were funded.

Not Available

1994-09-01T23:59:59.000Z

179

Transportation Agency Tool to Analyze Benefits of Living Snow Fences  

E-Print Network [OSTI]

Transportation Agency Tool to Analyze Benefits of Living Snow Fences 5/31/12 Transportation Agency/31/12 Transportation Agency Tool to Analyze Benefits of Living Snow Fences Center for Integrated Natural Resources, Mobility, & Transportation Authority Benefits, Farmer Costs, & Carbon Impacts Focus Groups and Surveys

Minnesota, University of

180

Carbon nanotubes as photoacoustic molecular imaging agents in living mice  

E-Print Network [OSTI]

Carbon nanotubes as photoacoustic molecular imaging agents in living mice ADAM DE LA ZERDA1 not shown to target a diseased site in living subjects. Here we show that single-walled carbon nanotubes of tumours. Intravenous administration of these targeted nanotubes to mice bearing tumours showed eight times

Khuri-Yakub, Butrus T. "Pierre"

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


181

"Space Station" Theme: Learning to Work, and Live, in Space  

E-Print Network [OSTI]

"Space Station" IMAX Film Theme: Learning to Work, and Live, in Space The educational value of NASM visit and afterward. See the "Alignment with Standards" table for details regarding how "Space Station in the "Space Station" program: · How astronauts train · What it is like to live and work in Space aboard

Mathis, Wayne N.

182

an interview with Richard Alba Extraordinary Lives: Patti Smith  

E-Print Network [OSTI]

When racial boundaries fade an interview with Richard Alba Extraordinary Lives: Patti Smith What on the workforce, the family, and American society. EXTRAORDINARY LIVES: PATTI SMITH 12 This was the first featured the maximally talented Patti Smith--singer, songwriter, artist and poet--who spoke thoughtfully

Dennehy, John

183

basic student budget 2014 |2015 LIVING ON CAMPUS  

E-Print Network [OSTI]

basic student budget 2014 |2015 #12;LIVING ON CAMPUS HALL FEES £ 119.18 ­ average cost x 40 weeks at £119.18 per week, and may be cheaper depending on type of accommodation. This budget is meant months (whether you live in the flat over the summer or not). This budget is meant as a realistic guide

Painter, Kevin

184

Living in the Netherlands 2.1 The Netherlands 24  

E-Print Network [OSTI]

Living in the Netherlands 2.1 The Netherlands 24 Higher education in the Netherlands 24 information 34 Expatdesk 34 23 #12;radboud universit y nijmegen 24 2.1 The Netherlands With more than 16 million people living in an area of some 41,000 square kilometres, the Netherlands is one of the most

van Suijlekom, Walter

185

Promoting Balance, Wellness & Fitness Creating healthier lives.  

E-Print Network [OSTI]

in student recruitment and retention. Engagement ­ We provide opportunities for students and members of their leisure time. Participation in such activities also assists students in performing well in a demanding interpersonal conflicts, learn healthy life-style habits, provide first aid and emergency response services

Washington at Seattle, University of

186

E-Print Network 3.0 - areas technical activities Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

conservation, such as sustainable use of forest... or marine resources Promote ecotourism and other income-generating activities for communities living near Source:...

187

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips  

DOE Patents [OSTI]

The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

2007-12-04T23:59:59.000Z

188

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips  

DOE Patents [OSTI]

The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

2007-10-30T23:59:59.000Z

189

9/4/12 Tigers Work the Night Shift in Nepal | LiveScience 1/8www.livescience.com/22876-tigers-humans-coexist.html  

E-Print Network [OSTI]

9/4/12 Tigers Work the Night Shift in Nepal | LiveScience 1/8www.livescience.com/22876-tigers Shift in Nepal #12;9/4/12 Tigers Work the Night Shift in Nepal | LiveScience 2/8www.livescience.com/22876-tigers-humans-coexist.html A Bengal tiger caught by a motion-activated camera in Nepal. CREDIT

190

Radiation-resistant microorganism  

DOE Patents [OSTI]

An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

Fliermans, Carl B.

2010-06-15T23:59:59.000Z

191

Genetically modified microorganisms for  

E-Print Network [OSTI]

-proteins. Thus far, Shewanella oneidensis and Geobacter sulfurreducens have been extensively studied into electricity in much more efficient ways. This chapter will discuss recent work on the investigation be possible in the near future. 6.2 EXTRACELLULAR RESPIRATION IN SHEWANELLA ONEIDENSIS AND GEOBACTER

Angenent, Lars T.

192

Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus  

E-Print Network [OSTI]

Background RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, a ...

Steglich, Claudia

193

Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus  

E-Print Network [OSTI]

Background: RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, ...

Steglich, Claudia

194

Long lived central engines in Gamma Ray Bursts  

E-Print Network [OSTI]

The central engine of Gamma Ray Bursts may live much longer than the duration of the prompt emission. Some evidence of it comes from the presence of strong precursors, post-cursors, and X-ray flares in a sizable fraction of bursts. Additional evidence comes from the fact that often the X-ray and the optical afterglow light curves do not track one another, suggesting that they are two different emission components. The typical "steep-flat-steep" behavior of the X-ray light curve can be explained if the same central engine responsible for the main prompt emission continues to be active for a long time, but with a decreasing power. The early X-ray "afterglow" emission is then the extension of the prompt emission, originating at approximately the same location, and is not due to forward shocks. If the bulk Lorentz factor Gamma is decreasing in time, the break ending the shallow phase can be explained, since at early times Gamma is large, and we see only a fraction of the emitting area. Later, when Gamma decreases, we see an increasing fraction of the emitting surface up to the time when Gamma ~ 1/theta_j. This time ends the shallow phase of the X-ray light curve. The origin of the late prompt emission can be the accretion of the fall-back material, with an accretion rate dot M proportional to t^(-5/3). The combination of this late prompt emission with the flux produced by the standard forward shock can explain the great diversity of the optical and the X-ray light curves.

G. Ghisellini

2008-10-01T23:59:59.000Z

195

Spontaneous fission half-lives for ground state nuclides  

SciTech Connect (OSTI)

Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

1991-01-01T23:59:59.000Z

196

University Housing Designing Sustainable Communities for Living and Learning  

E-Print Network [OSTI]

The Mission Learning Center for Sustainable Futures Green Learning Community Student Engagement Green Living Public Events Green Education University and Community Outreach Community Building Our Home:Physical Features LEED Certification Sustainable Sites Materials and Resources Energy and Atmosphere Indoor

Almor, Amit

197

LongLived Renaming Made Fast \\Lambda Harry Buhrman  

E-Print Network [OSTI]

Long­Lived Renaming Made Fast \\Lambda Harry Buhrman Centrum voor Wiskunde en Informatica (CWI) P.O. Box 94079, 1090 SB Amsterdam, The Netherlands buhrman@cwi.nl Juan A. Garay IBM T. J. Watson Research

Garay, Juan A.

198

The living commons : a spatial theory for biological design  

E-Print Network [OSTI]

Biological design is as ancient as human civilization. For thousands of years, living systems and natural processes have been manipulated by humans and their biological outcomes have been customized for different purposes. ...

Telhan, Orkan

2013-01-01T23:59:59.000Z

199

Ask a scientist: Nanotech in our lives | Argonne National Laboratory  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Ask a scientist: Nanotech in our lives June 1, 2014 Tweet EmailPrint Is there nanotechnology already in my consumer products? Carrado Gregar: I just saw a report that named...

200

Our Energy Independence- A Live Chat With Dr. Arun Majumdar  

Broader source: Energy.gov [DOE]

Join Dr. Arun Majumdar at 2 PM ET for a live, two-way conversation about the investments we're making to build the clean energy infrastructure of the future.

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


201

'Invisible lives' : the Gypsies and Travellers of Britain   

E-Print Network [OSTI]

This thesis examines the lives and experiences of relatively 'unknown' minority ethnic communities in Britain. As a group, they have been known by many names since their arrival in the 15th Century, although the most common ...

Clark, Colin Robert

2001-12-05T23:59:59.000Z

202

Live Webcast on Recent Wind Energy Technology Advances  

Broader source: Energy.gov [DOE]

The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

203

Range Vegetation Response to Burning Thicketized Live Oak Savannah.  

E-Print Network [OSTI]

TABLE 9. TOTAL STANDING CROP OF LIVE OAK AND NEW GROWTH (KGIHA) AND STANDING CROP INCREASE ( REPRESENTED BY NEW GROWTH IN APRIL AND JULY, 1* ON AREAS BURNED AT VARIOUS DATES ON THE ARANS NATIONAL WILDLIFE REFUGE NEAR AUSTWELL, TEXAS' New growth2... Response of live oak-dominated vegetation on thicketized uplands to burning in the fall 1974, spring 1975, and fall 1975 was evaluated through 1977 on the Aransas National Wildlife Refuge. Standing crop, species diversity, and botanical composition...

Scifres, C.J.; Kelly, D.M.

1979-01-01T23:59:59.000Z

204

LiveWall Operational Evaluation: Seattle Law Enforcement Pilot  

SciTech Connect (OSTI)

The LiveWall concept envisioned as an outgrowth of the Precision Information Environment (PIE) project allows communications between separate groups using interactive video, audio, and a shared desktop environment; this allows everyone to participate and collaborate in real time, regardless of location. The LiveWall concept provides a virtual window to other locations, where all parties can interact and collaboratively work with each other. This functionality is intended to improve multi-site coordination amongst emergency operations centers (EOC), field operations sites and across organizations and jurisdictions to accommodate communications during routine and emergency events. For the initial LiveWall operational evaluation PNNL partnered with the Seattle Police Department (SPD). This partnership allowed for the creation of an excellent LiveWall test bed specific to law enforcement. This partnership made it possible to test the LiveWall concept with scenarios involving the many facets of the law enforcement work done by SPD. PNNL and SPD agreed that integrating the systems into operations for a real event would be the best test of the technology and give SPD staff greater visibility into the functionality and benefits offered by the LiveWall concept.

Barr, Jonathan L.; Burtner, Edwin R.; Stein, Steven L.

2013-10-01T23:59:59.000Z

205

Plasma Enhanced Chemical Vapor Deposition on Living Substrates: Development, Characterization, and Biological Applications  

E-Print Network [OSTI]

This dissertation proposed the idea of “plasma-enhanced chemical vapor deposition on living substrates (PECVD on living substrates)” to bridge the gap between the thin film deposition technology and the biological and living substrates. This study...

Tsai, Tsung-Chan 1982-

2012-12-05T23:59:59.000Z

206

adult-to-adult living donor: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

D. G. Allen; C. R. Stanley; M. S. Sherwin 2005-03-05 92 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

207

Power Spectrum of Out-of-equilibrium Forces in Living Cells : Amplitude and Frequency Dependence  

E-Print Network [OSTI]

Living cells exhibit an important out-of-equilibrium mechanical activity, mainly due to the forces generated by molecular motors. These motor proteins, acting individually or collectively on the cytoskeleton, contribute to the violation of the fluctuation-dissipation theorem in living systems. In this work we probe the cytoskeletal out-of-equilibrium dynamics by performing simultaneous active and passive microrheology experiments, using the same micron-sized probe specifically bound to the actin cortex. The free motion of the probe exhibits a constrained, subdiffusive behavior at short time scales (t power law dependence with time. Combining the results of both experiments, we precisely measure for the first time the power spectrum of the force fluctuations exerted on this probe, which lies more than one order of magnitude above the spectrum expected at equilibrium, and greatly depends on frequency. We retrieve an effective temperature Teff of the system, as an estimate of the departure from thermal equilibrium. This departure is especially pronounced on long time scales, where Teff bears the footprint of the cooperative activity of motors pulling on the actin network. ATP depletion reduces the fluctuating force amplitude and results in a sharp decrease of Teff towards equilibrium.

Francois Gallet; Delphine Arcizet; Pierre Bohec; Alain Richert

2009-01-20T23:59:59.000Z

208

Influence of top removal on tebuthiuron efficacy for live oak control  

E-Print Network [OSTI]

/ha at several dates following shredding on April 14, 1983 near Edna, Texas. Tebuthiuron applied (months after shredding) Shredded Date pr1 1983 ct. pri 1984 Nov. Evaluation date 1985 uy9 No 10. 3 c-j 8. 6 a-i 10. 8 c-j 7. 7 a-h 9. 8 b-i Yes... control compared to response of undisturbed live oak. On April 14, 1983 forty plots were established half of which were shredded. Tebuthiuron at 2. 2 kg/ha as 3. 2 mm diameter pellets (20% active ingredient) was applied to undisturbed and shredded...

Swetish, Stephen Michael

1986-01-01T23:59:59.000Z

209

Harnessing microbial subsurface metal reduction activities to synthesise nanoscale cobalt ferrite with enhanced magnetic properties  

SciTech Connect (OSTI)

Nanoscale ferrimagnetic particles have a diverse range of uses from directed cancer therapy and drug delivery systems to magnetic recording media and transducers. Such applications require the production of monodisperse nanoparticles with well-controlled size, composition, and magnetic properties. To fabricate these materials purely using synthetic methods is costly in both environmental and economical terms. However, metal-reducing microorganisms offer an untapped resource to produce these materials. Here, the Fe(III)-reducing bacterium Geobacter sulfurreducens is used to synthesize magnetic iron oxide nanoparticles. A combination of electron microscopy, soft X-ray spectroscopy, and magnetometry techniques was employed to show that this method of biosynthesis results in high yields of crystalline nanoparticles with a narrow size distribution and magnetic properties equal to the best chemically synthesized materials. In particular, it is demonstrated here that cobalt ferrite (CoFe{sub 2}O{sub 4}) nanoparticles with low temperature coercivity approaching 8 kOe and an effective anisotropy constant of {approx} 10{sup 6} erg cm{sup -3} can be manufactured through this biotechnological route. The dramatic enhancement in the magnetic properties of the nanoparticles by the introduction of high quantities of Co into the spinel structure represents a significant advance over previous biomineralization studies in this area using magnetotactic bacteria. The successful production of nanoparticulate ferrites achieved in this study at high yields could open up the way for the scaled-up industrial manufacture of nanoparticles using environmentally benign methodologies. Production of ferromagnetic nanoparticles for pioneering cancer therapy, drug delivery, chemical sensors, catalytic activity, photoconductive materials, as well as more traditional uses in data storage embodies a large area of inorganic synthesis research. In particular, the addition of transition metals other than Fe into the structure of magnetite (Fe{sub 3}O{sub 4}) has been shown to greatly enhance the magnetic properties of the particles, tailoring them to different commercial uses. However, synthesis of magnetic nanoparticles is often carried out at high temperatures with toxic solvents resulting in high environmental and energy costs. Additionally, these ferrite nanoparticles are not intrinsically biocompatible, and to make them suitable for insertion into the human body is a rather intricate task. A relatively unexplored resource for magnetic nanomaterial production is subsurface Fe(III)-reducing bacteria, as these microorganisms are capable of producing large quantities of nanoscale magnetite (Fe{sub 3}O{sub 4}) at ambient temperatures. Metal-reducing bacteria live in environments deficient in oxygen and conserve energy for growth through the oxidation of hydrogen or organic electron donors, coupled to the reduction of oxidized metals such as Fe(III)-bearing minerals. This can result in the formation of magnetite via the extracellular reduction of amorphous Fe(III)-oxyhydroxides causing the release of soluble Fe(II) and resulting in complete recrystallization of the amorphous mineral into a new phase. Some previous studies have reported altering the composition of biogenic magnetite produced by Fe(III)-reducing bacteria for industrial and environmental applications. However, research into the commercial exploitation of bacteria to form magnetic minerals has focused primarily on magnetotactic bacteria which form magnetosomal magnetite internally using very different pathways to those bacteria forming magnetite outside the cell. Magnetotactic bacteria live at the sediment-water interface and use internal nanomagnets to guide them to their preferred environmental niche using the Earth's magnetic field. Since magnetotactic bacteria generally grow optimally under carefully controlled microaerobic conditions, the culturing processes for these organisms are challenging and result in low yields of nanomagnetite. Despite these limitations, magnetotactic bacteria have bee

Coker, Victoria S.; Telling, Neil D.; van der Laan, Gerrit; Pattrick, Richard A.D.; Pearce, Carolyn I.; Arenholz, Elke; Tuna, Floriana; Winpenny, Richard E.P.; Lloyd, Jonathan R.

2009-03-24T23:59:59.000Z

210

Long-Range Untethered Real-Time Live Gas Main Robotic Inspection System  

SciTech Connect (OSTI)

Under funding from the Department of Energy (DOE) and the Northeast Gas Association (NGA), Carnegie Mellon University (CMU) developed an untethered, wireless remote controlled inspection robot dubbed Explorer. The project entailed the design and prototyping of a wireless self-powered video-inspection robot capable of accessing live 6- and 8-inch diameter cast-iron and steel mains, while traversing turns and Ts and elbows under real-time control with live video feedback to an operator. The design is that of a segmented actively articulated and wheel-leg powered robot design, with fisheye imaging capability and self-powered battery storage and wireless real-time communication link. The prototype was functionally tested in an above ground pipe-network, in order to debug all mechanical, electrical and software subsystems, and develop the necessary deployment and retrieval, as well as obstacle-handling scripts. A pressurized natural gas test-section was used to certify it for operation in natural gas at up to 60 psig. Two subsequent live-main field-trials in both cast-iron and steel pipe, demonstrated its ability to be safely launched, operated and retrieved under real-world conditions. The system's ability to safely and repeatably exidrecover from angled and vertical launchers, traverse multi-thousand foot long pipe-sections, make T and varied-angle elbow-turns while wirelessly sending live video and handling command and control messages, was clearly demonstrated. Video-inspection was clearly shown to be a viable tool to understand the state of this critical buried infrastructure, irrespective of low- (cast-iron) or high-pressure (steel) conditions. This report covers the different aspects of specifications, requirements, design, prototyping, integration and testing and field-trialing of the Explorer platform.

Hagen Schempf; Daphne D'Zurko

2004-10-31T23:59:59.000Z

211

Life in the Living Laboratory: An Anthropological Investigation of Environmental Science, Tourism, and Design in the Contemporary Bahamas  

E-Print Network [OSTI]

envisioned through sustainable designs for living. It can bethe living laboratory- biological, sustainable, ecologicalsustainable economic development- processes of human life and living,

Moore, Amelia M.

2010-01-01T23:59:59.000Z

212

Development of a Multiplexed, Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples. Final Report  

SciTech Connect (OSTI)

This was the final report for DOE NABIR grant DE-FG02-01ER63264 (PI Mary Lowe). The grant was entitled ''Development of a Multiplexed Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples.'' The grant duration was one year. The purpose was to develop a bead-based assay for measuring analyte DNAs in environmental PCR products and to apply the method to a field experiment. The primary experiment was located at the UMTRA Old Rifle site.

Lowe, M.; Halden, R.

2002-10-09T23:59:59.000Z

213

The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living a core experience for First  

E-Print Network [OSTI]

The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living University. The Sustainability community infuses the learning-living community with practical and theoretical approaches to sustainable living, merging students' living community with unique academic and field

214

Activated sludge process: Waste treatment. (Latest citations from the Biobusiness database). Published Search  

SciTech Connect (OSTI)

The bibliography contains citations concerning the use of the activated sludge process in waste and wastewater treatment. Topics include biochemistry of the activated sludge process, effects of various pollutants on process activity, effects of environmental variables such as oxygen and water levels, and nutrient requirements of microorganisms employed in activated sludge processes. The citations also explore use of the process to treat specific wastes, such as halocarbons, metallic wastes, and petrochemical effluents; and wastes from pharmaceutical and dairy processes. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

NONE

1996-01-01T23:59:59.000Z

215

http://tti.tamu.edu Saving Lives, Time and Resources  

E-Print Network [OSTI]

and organization > Project management > Education and training > Costs > Capital investments > Road pricinghttp://tti.tamu.edu Saving Lives, Time and Resources A Member of The Texas A&M University System estimation > Traffic forecasting > Commuting > Carpools > Highway operations > Highway capacity > Freeway

216

Student Accommodation and Living Guide 2012/2013  

E-Print Network [OSTI]

a couple of miles away, which is home to both beautiful historic buildings and highly sustainable state to sustainable living that has won our campus the award for `Outstanding Contribution to Sustainable Development.bradford.ac.uk/accommodation/ Address: Accommodation Services "The Orchard", The Green University of Bradford Bradford West Yorkshire BD

Zharkova, Valentina V.

217

Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE  

E-Print Network [OSTI]

Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE ENVIRONMENT Environmental Studies 50 steps necessary for this goal. We begin by defining "green building" and explaining its relevance to the College's plans for new residences. Green building incorporates sensitivity towards the environment

218

Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE  

E-Print Network [OSTI]

Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE ENVIRONMENT Environmental Studies 50 Recommendations 3 a. Chapters I and II: Green Building and New Dorms 3 b. Chapter III: Existing Dorms 3 c. Chapter Institutions 5 CHAPTER 2: GREEN BUILDING 6 CHAPTER 3: NEW DORMS 9 I. The Need for New Housing 10 II. Overview

219

Performance and Energy Modeling for Live Migration of Virtual Machines  

E-Print Network [OSTI]

Performance and Energy Modeling for Live Migration of Virtual Machines Haikun Liu , Cheng-Zhong Xu , Hai Jin , Jiayu Gong , Xiaofei Liao School of Computer Science and Technology Huazhong University of Science and Technology Wuhan, 430074, China {hjin, xfliao}@hust.edu.cn Department of Electrical

Xu, Cheng-Zhong

220

Aptamer Directly Evolved from Live Cells Recognizes Membrane Bound Immunoglobin  

E-Print Network [OSTI]

. These include cell signaling, cell-cell interactions, ion/solute transport that facilitates the exchangeAptamer Directly Evolved from Live Cells Recognizes Membrane Bound Immunoglobin Heavy Mu Chain, and Weihong Tan The identification of tumor related cell membrane protein targets is important

Tan, Weihong

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


221

Live in Spain while earning academic credit Spring 2013  

E-Print Network [OSTI]

+ + + Live in Spain while earning academic credit Spring 2013 · Study at one of Spain's oldest Spain with other CSU students · Take advantage of the opportunities you will have to travel throughout Spain & greater Europe Centrally located 20 miles from the vibrant city of Madrid, Alcalá de Henares

222

LIVE. WORK. CELEBRATE. Email order to: Beverlyhills@cort.com  

E-Print Network [OSTI]

and Lamp LIVING ROOM: Sofa, Chair, Cocktail Table, End Table, Lamp, plus 32" LCD TV with Stand STUDY: Chair TV with Stand, Accent Pillows and 3 Pieces of Artwork STUDY: Chair and Lamp DINING ROOM: Dining Table drop it off, set it up and pick it up n No heavy lifting or assembly required n Fit a student

Williams, Gary A.

223

Framing Change: Social Movement Framing in University Living Wage Movements  

E-Print Network [OSTI]

of these living wage movements, two cases, Texas A&M University and Georgetown University, were selected for this study to examine through the lens of the social movement framing perspective. Data for the cases included interviews with activists and administrators...

Metcalf, Laurie D.

2010-01-14T23:59:59.000Z

224

Optimized Pre-Copy Live Migration for Memory Intensive Applications  

E-Print Network [OSTI]

Optimized Pre-Copy Live Migration for Memory Intensive Applications Khaled Z. Ibrahim, Steven Hofmeyr, Costin Iancu, Eric Roman Lawrence Berkeley National Laboratory Email: {kzibrahim, shofmeyr and fault tolerance. KVM and Xen use iterative pre-copy approaches which work well in practice

225

International Year of the Rhino Living Planet Report 2012  

E-Print Network [OSTI]

in South Africa, WWF invests an incredible amount of time and resources into the protection of our iconicInternational Year of the Rhino Living Planet Report 2012 A `Decisive' victory for ethical food by the announcement that June 2012 marked the start of the International Year of the Rhino. Internationally and here

de Villiers, Marienne

226

MFR PAPER 1060 Success of the live-bait tuna  

E-Print Network [OSTI]

-bait albacore fis heries are in temperate waters . The discussion of live-bait tuna fis hi ng in th is paper wi and tempera- ture change so that the bai t can be carried by the fishing boat from one fis hi ng ground to another through waters of varying temperatures. The preferred bai tfishes for tuna fis hing are c1upeoids

227

Vertebrate heart development: Lessons learnt from live imaging  

E-Print Network [OSTI]

Vertebrate heart development: Lessons learnt from live imaging California Institute of Technology employing different imaging techniques. Sub resolution imaging of beating zebrafish heart has however remained a challenge owing Embryonic heart is a 100 moving quasi-periodically at few Hertz frequency, over

Shyamasundar, R.K.

228

Safety and Liveness in Intelligent Intersections Hemant Kowshik1  

E-Print Network [OSTI]

of designing intelligent intersections where traffic lights and stop signs are removed, and cars negotiate intersections are representative of complex distributed hybrid systems which need architectures and algorithms with provable safety and liveness. We propose a hybrid architecture which involves an appropriate inter- play

229

ith America focused on energy alternatives and green living,  

E-Print Network [OSTI]

W ith America focused on energy alternatives and green living, Oklahoma State University- Tulsa. His process to produce a clean, alternative energy source could significantly reduce the use of fossil it a very attractive alternative to solar energy technology. "Thermoelectric materials are so much less

Veiga, Pedro Manuel Barbosa

230

Women in management: gender, age and working lives   

E-Print Network [OSTI]

The project reported in this briefing paper explored how age and gender inter-weave and impact on the working and home lives of women managers working in two EU member states, namely Finland and Scotland. The proportion of women in middle...

Jyrkinen, Marjut; McKie, Linda

231

The importance of live biofilms in corrosion protection  

E-Print Network [OSTI]

Letter The importance of live biofilms in corrosion protection Rongjun Zuo a , Esra Kus b , Florian, University of Connecticut, 191 Auditorium Road, U-3222, Storrs, CT 06269-3222, USA b Corrosion occurred within a few hours as indicated by characteristic changes in the impedance spectra. The corrosion

Wood, Thomas K.

232

Healthy Foods, Healthy Lives: Cooking on a Student's Budget  

E-Print Network [OSTI]

Healthy Foods, Healthy Lives: Cooking on a Student's Budget FScN 2002 ­ Fall & Spring Semesters/monthly menus within a realistic food budget and a modest supply list Gain valuable information and connect to a variety of resources on nutrition, food safety, budgeting and meal planning Develop a social network

Amin, S. Massoud

233

Residential Services Area Missing Students living in University Managed Accommodation  

E-Print Network [OSTI]

Residential Services Area Missing Students living in University Managed Accommodation 1.0 Where then report to the Building Manager or to the Residential Student Support Team or the Residential Services issues Residential Student Support Manager or the Residential Services Manager should be contacted

Sussex, University of

234

THE GREEN GUIDE Your guide to sustainable living  

E-Print Network [OSTI]

THE GREEN GUIDE 2012-2013 Your guide to sustainable living at UC San Diego, brought to you by the HDH Sustainability Econauts #12;! 1.Limit what you purchase to what you need. 2.Find clever. The little things make a big difference! 3.Remember to keep yourself hydrated! The most sustainable way to do

Tsien, Roger Y.

235

Mount Baker--Living with an Active Volcano U.S. Department of the Interior  

E-Print Network [OSTI]

because of condensation of steam in cold, calm air. In 1975, however, increased steaming and melting is molten rock (magma) that pours or oozes onto the Earth's surface. Numerous eruptions of lava interbedded east side with steam plume rising from Sherman Crater (left side of summit, inset shows crater interior

Torgersen, Christian

236

Fossil identification activity Standard: Comparing fossils to each other or to living organisms reveals features of  

E-Print Network [OSTI]

. Icaronycteris XI6a, aka" bat" 4. Boney Fish (Knightera) XI2c, (1 piece) 5. Megalodon tooth XIa 6. Dinosaur skin? (teeth? skin? bone? imprint?) 3. What kind of animal is it? (reptile? mammal? invertebrate? fish?) 4. Why

237

E-Print Network 3.0 - active living logan Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

University Collection: Biology and Medicine 69 ENVIRONMENTAL BIOTECHNOLOGY Brewery wastewater treatment using air-cathode Summary: Yujie Feng & Xin Wang & Bruce E. Logan & He Lee...

238

Mechanical and free living comparisons of four generations of the Actigraph activity monitor  

E-Print Network [OSTI]

of the cut-points related to the energy consumption. How- ever, increasing the epoch time could blunt the response to especially higher PA intensities [28]. It would therefore an advantage to employ short epochs when evaluating the dif- ferences between... and down- loaded via a serial port interface using a DOS-based pro- gram (RUI24, v. 2.13B, Computer Science and Applications INC.) and the newer monitor generations were initialized and downloaded via a USB interface using the Actilife soft- ware (v. 5...

Ried-Larsen, Mathias; Brønd, Jan C; Brage, Søren; Hansen, Bjørge H; Grydeland, May; Andersen, Lars B; Møller, Niels C

2012-09-12T23:59:59.000Z

239

activity-long living wastes: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

the appeal of some scientific disciplines. Sparavigna, A C 2012-01-01 7 Municipal solid waste characteristics and management in Allahabad, India Renewable Energy Websites...

240

Short- and long-lived radionuclide particle size measurements in a uranium mine  

SciTech Connect (OSTI)

The radon-222 progeny and long-lived radionuclide measurements were done in a wet underground uranium mine in Saskatchewan, Canada, on Nov. 8-12, 1995. Radon-222 in the mine varied from 2 kBq/m{sup 3} at 90 m below surface to 12 kBq/m{sup 3} in the mining areas, 240 m below surface. Radon-222 progeny activity and potential alpha energy concentration appear affected by the airborne particle number concentration and size distribution. Particle number was up to 200x10{sup 3}/cm{sup 3}. Only an accumulation mode (30-1000 nm) and some bimodal size distributions in this accumulation size range were significant. Diesel particles and combustion particles from burning propane caused a major modal diameter shift to a smaller size range (50-85 nm) compared with previous values (100-200 nm). The high particle number reduced the unattached progeny (0.5-2 nm) to >5%. The nuclei mode (2-30 nm) in this test was nonexistent, and the coarse mode (>1000 nm), except from the drilling areas and on the stopes, was mostly not measurable. Airborne particle total mass and long- lived radionuclide alpha activity concentrations were very low (80- 100 {mu}g/m{sup 3} and 4-5 mBq/m{sup 3}) owing to high ventilation rates. Mass-weighted size distributions were trimodal, with the major mode at the accumulation size region, which accounts for 45-50% of the mass. The coarse model contains the the least mass, about 20%. The size spectra from gross alpha activities were bimodal with major mode in the coarse region (>1000 nm) and a minor accumulation mode in the 50-900 nm size range. These size spectra were different from the {sup 222}Rn progeny that showed a single accumulation mode in the 50- 85 nm size region. The accumulation mode in the long-lived radionuclide size spectrum was not found in previous studies in other uranium mines.

Tu, Keng-Wu; Fisenne, I.M.; Hutter, A.R.

1997-04-01T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


241

Prompt gamma activation analysis (PGAA) and short-lived neutron activation analysis (NAA) applied to the characterization of legacy materials  

E-Print Network [OSTI]

relative to the linear axis of the neutron generator and toto the linear axis of the neutron generator. To protectto the linear axis of the neutron generator) to reduce the

English, G.A.

2008-01-01T23:59:59.000Z

242

NUCLEAR HALF-LIVES FOR ALPHA RADIOACTIVITY OF ELEMENTS WITH  

E-Print Network [OSTI]

Theoretical estimates for the half lives of about 1700 isotopes of heavy elements with 100 ? Z ? 130 are tabulated using theoretical Q-values. The quantum mechanical tunneling probabilities are calculated within a WKB framework using microscopic nuclear potentials. The microscopic nucleus- nucleus potentials are obtained by folding the densities of interacting nuclei with a density dependent M3Y (DDM3Y) effective nucleon- nucleon interaction. The ?-decay half lives calculated in this formalism using the experimental Q-values were found to be in good agreement over a wide range of experimental data spanning about twenty orders of magnitude. The theoretical Q-values used for the present calculations are extracted from three different mass estimates viz. Myers-Swiatecki [MS], Muntian-Hofmann-Patyk-

P. Roy Chowdhury A; C. Samanta A

243

The Sun is an active, variable,  

E-Print Network [OSTI]

The Sun is an active, variable, magnetic star with oscillations on its surface and deep within its the physical processes of the Sun-Earth connection. Visit Us on the Web: Solar Terrestrial Probes Program: http://stp.gsfc.nasa.gov For More Information, See: Sun-Earth Connection: http://sec.gsfc.nasa.gov Living With a Star: http

Christian, Eric

244

27 | P a g e Activity 6  

E-Print Network [OSTI]

sustainable living and reduced ecological impacts · An increased sense of community and the creation 27 | P a g e Activity 6 The Commuter Challenge Goals: · Motivate sustainable action on campus · Introduce sustainability concepts using a fun, interactive approach Outcomes: · Improved

Brownstone, Rob

245

Uncharted Microbial World: Microbes and Their Activities in the Environment  

SciTech Connect (OSTI)

Microbes are the foundation for all of life. From the air we breathe to the soil we rely on for farming to the water we drink, everything humans need to survive is intimately coupled with the activities of microbes. Major advances have been made in the understanding of disease and the use of microorganisms in the industrial production of drugs, food products and wastewater treatment. However, our understanding of many complicated microbial environments (the gut and teeth), soil fertility, and biogeochemical cycles of the elements is lagging behind due to their enormous complexity. Inadequate technology and limited resources have stymied many lines of investigation. Today, most environmental microorganisms have yet to be isolated and identified, let alone rigorously studied. The American Academy of Microbiology convened a colloquium in Seattle, Washington, in February 2007, to deliberate the way forward in the study of microorganisms and microbial activities in the environment. Researchers in microbiology, marine science, pathobiology, evolutionary biology, medicine, engineering, and other fields discussed ways to build on and extend recent successes in microbiology. The participants made specific recommendations for targeting future research, improving methodologies and techniques, and enhancing training and collaboration in the field. Microbiology has made a great deal of progress in the past 100 years, and the useful applications for these new discoveries are numerous. Microorganisms and microbial products are now used in industrial capacities ranging from bioremediation of toxic chemicals to probiotic therapies for humans and livestock. On the medical front, studies of microbial communities have revealed, among other things, new ways for controlling human pathogens. The immediate future for research in this field is extremely promising. In order to optimize the effectiveness of community research efforts in the future, scientists should include manageable systems with features like clear physical boundaries, limited microbial diversity, and manipulability with the goal of understanding fundamental principles that may apply to more complex systems. A great deal of microbial genetic and phenotypic diversity remains to be explored, and the commercial and medical potential locked up in these unknowns should compel the field to move forward. Future microbiology research will build on the successes of the past using new techniques and approaches. Uncultivated microbes hold great promise for industry, medicine, and the recycling of precious resources, and research and technology must make inroads in overcoming the barriers that prevent their study. In many cases, we will no longer be able to rely on isolated, pure cultures of microorganisms, but must use communities of microorganisms, which presently are poorly understood. Indeed, community-level studies can benefit from deconstructing microbial communities and analyzing the component members separately, but this is not feasible in every system. The effects of perturbation on microbial communities also require study. Humans rely on the services of microbes in innumerable ways, but we have little or no predictive understanding of how microbial communities respond to disturbance. Research must address current limitations in detecting microscale interactions among microbes by enhancing current technologies and fostering new microscopic tools, biosensors, and gas sensors for appropriate small scales. Genomics, which has enabled great progress in microbiology research of individual species, must be applied to communities of microorganisms. This will require improved methods of DNA extraction and amplification from environmental samples and improved strategies for DNA sequence assembly. In the future, genome sequencing efforts should continue the exploration of evolutionarily diverse microbes, as well as help reveal the mechanisms by which closely related microbes evolve. Technological advances have spurred every great leap in microbial biology, and in order to move forward, new m

Harwood, Caroline; Buckley, Merry.

2007-12-31T23:59:59.000Z

246

Single-photon ultrashort-lived radionuclides: symposium proceedings  

SciTech Connect (OSTI)

The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

Paras, P.; Thiessen, J.W. (eds.)

1985-01-01T23:59:59.000Z

247

Dynamic self-assembly in living systems as computation.  

SciTech Connect (OSTI)

Biochemical reactions taking place in living systems that map different inputs to specific outputs are intuitively recognized as performing information processing. Conventional wisdom distinguishes such proteins, whose primary function is to transfer and process information, from proteins that perform the vast majority of the construction, maintenance, and actuation tasks of the cell (assembling and disassembling macromolecular structures, producing movement, and synthesizing and degrading molecules). In this paper, we examine the computing capabilities of biological processes in the context of the formal model of computing known as the random access machine (RAM) [Dewdney AK (1993) The New Turing Omnibus. Computer Science Press, New York], which is equivalent to a Turing machine [Minsky ML (1967) Computation: Finite and Infinite Machines. Prentice-Hall, Englewood Cliffs, NJ]. When viewed from the RAM perspective, we observe that many of these dynamic self-assembly processes - synthesis, degradation, assembly, movement - do carry out computational operations. We also show that the same computing model is applicable at other hierarchical levels of biological systems (e.g., cellular or organism networks as well as molecular networks). We present stochastic simulations of idealized protein networks designed explicitly to carry out a numeric calculation. We explore the reliability of such computations and discuss error-correction strategies (algorithms) employed by living systems. Finally, we discuss some real examples of dynamic self-assembly processes that occur in living systems, and describe the RAM computer programs they implement. Thus, by viewing the processes of living systems from the RAM perspective, a far greater fraction of these processes can be understood as computing than has been previously recognized.

Bouchard, Ann Marie; Osbourn, Gordon Cecil

2004-06-01T23:59:59.000Z

248

ClimateChangeLIVE Webcast: Join the Climate Conversation  

Broader source: Energy.gov [DOE]

Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

249

Live Oak County, Texas: Energy Resources | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429Lacey,(Monaster AndLittletown, Arizona: Energy Resources Jump to:Live Oak

250

JV between KP Renewables and Living Buildings | Open Energy Information  

Open Energy Info (EERE)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia: Energy Resources Jump to:46 - 429 Throttled (botOpen Energy2005) |JMalucelli Energia Place:Living Buildings Jump

251

The problem of living in a world contaminated with chemicals  

SciTech Connect (OSTI)

The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

Metcalf, R.L. [Univ. of Illinois, Urbana (United States)

1990-12-31T23:59:59.000Z

252

Half-lives of Double $?^+$-decay with Two Neutrinos  

E-Print Network [OSTI]

Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated half-lives of double $\\beta ^+$-decay in this article will be useful for future experimental search of double $\\beta ^+$-decay.

Yuejiao Ren; Zhongzhou Ren

2015-01-07T23:59:59.000Z

253

Arsenic exposure in children living near a former copper smelter  

SciTech Connect (OSTI)

About 10,000 people live in communities surrounding the former copper smelter at Anaconda, Montana. Most of these people live in the town of Anaconda, which is generally upwind of the smelter. The smelter ceased operations in 1980, after almost a century of ore processing. Soil and dust on the smelter site and in the vicinity remain contaminated with arsenic, although at this time air and drinking water arsenic levels are not elevated. Results of soil and dust sampling for arsenic in the communities around the smelter are reported. In the town of Anaconda, surface soil arsenic levels from residential sites have averaged around 100 ppm or greater. Young children are generally believed to be the population with the most nonoccupational exposure to soil. Several models of exposure to environmental arsenic in the Anaconda area have predicted that children living in all communities surrounding the smelter would be having significant and measurable exposure to arsenic. Two exposures surveys, conducted while the smelter was operative, demonstrated that excess exposure to arsenic was occurring in young children. Until the present surveys, no exposure data had been collected since the smelter was closed.

Binder, S.; Forney, D.; Kaye, W.; Paschal, D.

1987-07-01T23:59:59.000Z

254

Mass Spectral Molecular Networking of Living Microbial Colonies  

SciTech Connect (OSTI)

Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a "holy grail" in microbiology. This work describes a highly sensitive, broadly applicable, and costeffective approach that allows metabolic profiling of live microbial colonies directly from a Petri dish without any sample preparation. Nanospray desorption electrospray ionization mass spectrometry (MS), combined with alignment of MS data and molecular networking, enabled monitoring of metabolite production from live microbial colonies from diverse bacterial genera, including Bacillus subtilis, Streptomyces coelicolor, Mycobacterium smegmatis, and Pseudomonas aeruginosa. This work demonstrates that, by using these tools to visualize small molecular changes within bacterial interactions, insights can be gained into bacterial developmental processes as a result of the improved organization of MS/MS data. To validate this experimental platform, metabolic profiling was performed on Pseudomonas sp. SH-C52, which protects sugar beet plants from infections by specific soil-borne fungi [R. Mendes et al. (2011) Science 332:1097–1100]. The antifungal effect of strain SHC52 was attributed to thanamycin, a predicted lipopeptide encoded by a nonribosomal peptide synthetase gene cluster. Our technology, in combination with our recently developed peptidogenomics strategy, enabled the detection and partial characterization of thanamycin and showed that it is amonochlorinated lipopeptide that belongs to the syringomycin family of antifungal agents. In conclusion, the platform presented here provides a significant advancement in our ability to understand the spatiotemporal dynamics of metabolite production in live microbial colonies and communities.

Watrous, Jeramie D.; Roach, Patrick J.; Alexandrov, Theodore; Heath, Brandi S.; Yang, Jane Y.; Kersten, Roland; vander Voort, Menno; Pogliano, Kit; Gross, Harald; Raaijmakers, Jos M.; Moore, Bradley S.; Laskin, Julia; Bandeira, Nuno; Dorrestein, Pieter C.

2012-06-26T23:59:59.000Z

255

Phospholamban mutants compete with wild type for SERCA binding in living cells  

SciTech Connect (OSTI)

Highlights: Black-Right-Pointing-Pointer PLB phosphorylation in HEK cells increased FRET between YFP-PLB and CFP-SERCA. Black-Right-Pointing-Pointer Competition: Expressing loss-of-function PLB mutants in the system decreased FRET. Black-Right-Pointing-Pointer The FRET assay could screen potential therapeutic PLB mutants to activate SERCA. -- Abstract: We have used fluorescent fusion proteins stably expressed in HEK cells to detect directly the interaction between the sarcoplasmic reticulum Ca-ATPase (SERCA) and phospholamban (PLB) in living cells, in order to design PLB mutants for gene therapy. Ca{sup 2+} cycling in muscle cells depends strongly on SERCA. Heart failure (HF), which contributes to 12% of US deaths, typically exhibits decreased SERCA activity, and several potential therapies for HF aim to increase SERCA activity. We are investigating the use of LOF-PLB mutants (PLB{sub M}) as gene therapy vectors to increase SERCA activity. Active SERCA1a and WT-PLB, tagged at their N termini with fluorescent proteins (CFP and YFP), were coexpressed in stable HEK cell lines, and fluorescence resonance energy transfer (FRET) was used to detect their interaction directly. Phosphorylation of PLB, induced by forskolin, caused an increase in FRET from CFP-SERCA to YFP-PLB, indicating that SERCA inhibition can be relieved without dissociation of the complex. This suggests that a LOF mutant might bind to SERCA with sufficient affinity to complete effectively with WT-PLB, thus relieving SERCA inhibition. Therefore, we transiently expressed a series of PLB{sub M} in the CFP-SERCA/YFP-PLB cell line, and found decreased FRET, implying competition between PLB{sub M} and WT-PLB for binding to SERCA. These results establish this FRET assay as a rapid and quantitative means of screening PLB{sub M} for optimization of gene therapy to activate SERCA, as needed for gene therapy in HF.

Gruber, Simon J.; Haydon, Suzanne [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)] [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Thomas, David D., E-mail: ddt@umn.edu [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)

2012-04-06T23:59:59.000Z

256

Live Imaging Study on Cytokinin Function and Regulation in Stem-cell Homeostasis  

E-Print Network [OSTI]

on research on SAM Live-image technology was developed toin SAMs, transient perturbations, live image and cell typetreated. (C) The images from the same SAM were used to score

Xie, Mingtang

2010-01-01T23:59:59.000Z

257

E-Print Network 3.0 - aboveground live biomass Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

live biomass Search Powered by Explorit Topic List Advanced Search Sample search results for: aboveground live biomass Page: << < 1 2 3 4 5 > >> 1 Aerial Photo: P. velutina cover...

258

TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...  

Office of Environmental Management (EM)

TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

259

Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals  

DOE Patents [OSTI]

Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

2009-04-28T23:59:59.000Z

260

Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow...  

Broader source: Energy.gov (indexed) [DOE]

Summit Live: 9:30-10 AM ET Today and All Day Tomorrow Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow May 4, 2011 - 8:00am Addthis Ginny Simmons Ginny Simmons Former...

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


261

How do Communities Change their Culture Towards more Sustainable Patterns of Living, Working and Learning?  

E-Print Network [OSTI]

How do Communities Change their Culture Towards more Sustainable Patterns of Living, Working towards more sustainable ways of living; a case study of the Sustainability Office's efforts to change Staff: Heather Scholefield, Strategy Manager, Sustainability Office, Land and Building Services

262

The Living Building Challenge v1.0 In Pursuit of True Sustainability in the Built Environment  

E-Print Network [OSTI]

. McLennan · email: jason@cascadiagbc.org · Draft Living Building Standard #12;NOTIFICATION The Living: jason@cascadiagbc.org · Draft Living Building Standard #12;Table of Contents The Living Building Challenge 2 Executive Summary 2 How The Living Building Standard Works 4 Site Design 6 Energy 8 Materials

Zaferatos, Nicholas C.

263

Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent longevity  

E-Print Network [OSTI]

Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent 31905, Israel Summary Naked mole-rats (Heterocephalus glaber), the longest-lived rodents, live 7% of their lives. Little is known about the mechanisms that allow them to delay the aging process and live so long

Huchon, Dorothée

264

C3c concentrations in sera of persons living in areas with different levels of air pollution in Northrhine-Westphalia (Federal Republic of Germany)  

SciTech Connect (OSTI)

A number of air pollutants is known to affect the immune system resulting in alterations of the complement activity in serum. We determined the levels of C3c, one of the activation products of the component C3, in serum samples obtained from various population groups living in areas with different levels of air pollution in Northrhine-Westphalia (FRG). The results show that the serum C3c levels tend to be increased in subjects living in areas with elevated levels of air pollution. By applying a linear regression model it can be demonstrated that a significant part of the variance of the C3c concentrations is explained by the residence area factor after making allowance for the effect of various confounders. The increase of the serum C3c concentrations appears to result from an increased activation of the complement component C3. The underlying mechanisms as well as the pathophysiological significance of these findings are unknown.

Stiller-Winkler, R.; Kraemer, U.F.; Fiedler, E.; Ewers, U.; Dolgner, R.

1989-06-01T23:59:59.000Z

265

Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University  

E-Print Network [OSTI]

Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University Oct 14, 2009 #12;Intelligent Transportation Systems: Saving Lives, Time and Money James Whiteneck #12;Intelligent Transportation Systems: Saving Lives, Time and Money Portal archives a large amount of data Over

Bertini, Robert L.

266

1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee  

E-Print Network [OSTI]

1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee Initial Meeting January 22, 2009 #12;2Intelligent Transportation Systems: Saving Lives, Time and Money Agenda 9:00 Adjourn #12;3Intelligent Transportation Systems: Saving Lives, Time and Money What's in the PORTAL

Bertini, Robert L.

267

Live Help Systems: An Approach to Intelligent Help for Web Information  

E-Print Network [OSTI]

Live Help Systems: An Approach to Intelligent Help for Web Information Systems Johan °Aberg #12, and it is increasingly important to provide them with support. Our research approach to online help for Web site users is the intro- duction and study of what we call live help systems. A live help system is an intelligent help

Aberg, Johan

268

The Living Site and Infrastructure Challenge In pursuit of true sustainability in the built environment  

E-Print Network [OSTI]

The Living Site and Infrastructure Challenge In pursuit of true sustainability in the built environment Draft Version 1.0 November 2007 #12;NOTIFICATION The Living Site and Infrastructure Challenge - copyright 2007 - Cascadia Region Green Building Council, all rights reserved. The Living Site

Zaferatos, Nicholas C.

269

activity policy environments: Topics by E-print Network  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

activity policy environments First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Connecting Active Living...

270

Production of short lived radioactive beams of radium  

E-Print Network [OSTI]

Short lived $^{212,213,214}$Ra isotopes have been produced at the TRI$\\mu$P facility in inverse kinematics via the fusion-evaporation reaction $^{206}$Pb+$^{12}$C at 8 MeV/u. Isotopes are separated from other reaction products online using the TRI$\\mu$P magnetic separator. The energetic radium (Ra) isotopes at the exit of the separator were converted into low energy ions with a thermal ionizer. Ra isotopes have been identified by observing their $\\alpha$ decay and life times.

Shidling, P D; van der Hoek, D J; Jungmann, K; Kruithof, W; Onderwater, C J G; Sohani, M; Versolato, O O; Willmann, L; Wilschut, H W

2009-01-01T23:59:59.000Z

271

Production of short lived radioactive beams of radium  

E-Print Network [OSTI]

Short lived $^{212,213,214}$Ra isotopes have been produced at the TRI$\\mu$P facility in inverse kinematics via the fusion-evaporation reaction $^{206}$Pb+$^{12}$C at 8 MeV/u. Isotopes are separated from other reaction products online using the TRI$\\mu$P magnetic separator. The energetic radium (Ra) isotopes at the exit of the separator were converted into low energy ions with a thermal ionizer. Ra isotopes have been identified by observing their $\\alpha$ decay and life times.

P. D. Shidling; G. S. Giri; D. J. van der Hoek; K. Jungmann; W. Kruithof; C. J. G. Onderwater; M. Sohani; O. O. Versolato; L. Willmann; H. W. Wilschut

2009-02-25T23:59:59.000Z

272

Probing the membrane potential of living cells by dielectric spectroscopy  

E-Print Network [OSTI]

In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtain from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli were chosen for our experiments.

Corina Bot; Camelia Prodan

2008-12-17T23:59:59.000Z

273

Origin of Long Lived Coherences in Light-Harvesting Complexes  

E-Print Network [OSTI]

A vibronic exciton model is developed to investigate the origin of long lived coherences in light-harvesting complexes. Using experimentally determined parameters and uncorrelated site energy fluctuations, the model predicts oscillations in the nonlinear spectra of the Fenna-Matthews-Olson (FMO) complex with a dephasing time of 1.3 ps at 77 K. These oscillations correspond to the coherent superposition of vibronic exciton states with dominant contributions from vibrational excitations on the same pigment. Purely electronic coherences are found to decay on a 200 fs timescale.

Niklas Christensson; Harald F. Kauffmann; Tonu Pullerits; Tomas Mancal

2012-01-30T23:59:59.000Z

274

How Will We Live Forever? | GE Global Research  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-SeriesFlickr FlickrGuidedCH2MLLC HistoryVeterans |VirtualLove Be Deeper?Live

275

Tracking Living Cells as They Differentiate in Real Time  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsrucLas ConchasPassiveSubmittedStatusButler Tina ButlerToday inm"TopoTracking Living Cells as

276

Live from the Lab - SPORT Video | The Ames Laboratory  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces and Interfaces Sample6, 2011LiisaInnovation Portal SolarLittleageForLive

277

Live webcast on groundbreaking results of Algal Biofuels Consortium, June  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-SeriesFlickrinformationPostdocsCenterCentera A B C D E F G H ILive CellLive11

278

Tracking Living Cells as They Differentiate in Real Time  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonalTracking Living Cells as They

279

Tracking Living Cells as They Differentiate in Real Time  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonalTracking Living Cells as

280

Tracking Living Cells as They Differentiate in Real Time  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism in Layered NbS2Topo II: An EnzymePersonalTracking Living Cells

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


281

Firearm equipped with live round inhibiting means and method of making same  

DOE Patents [OSTI]

A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means. 9 figs.

Baehr, D.G.

1990-11-13T23:59:59.000Z

282

Towards Plasma Surgery: Plasma Treatment of Living Cells  

SciTech Connect (OSTI)

The physical, biological and technical background for high-precision plasma surgery is prepared in a multi-disciplinary team. The aim of the research is to achieve controlled removal of diseased cells and bacteria without harming the healthy rest of the tissue. For this purpose, a small, cold, flexible and non-toxic plasma is developed (the plasma needle) and tested on cultured cells and bacterial samples. The needle is an atmospheric discharge induced by a radio-frequency voltage applied to a metal pin. This plasma operates at room temperature, in the milliwatt power regime; it poses no risk of thermal or electrical damage to living tissues. Several beneficial responses of living cells to plasma treatment have been already identified. Plasma does not cause accidental cell death (necrosis), which leads to inflammation and tissue damage. Instead, it allows to detach cells from each other and from the scaffold, and thus to remove them in a non-destructive way. Moreover, plasma is capable of bacterial inactivation. I parallel, we have determined the electrical and optical properties of the plasma and found a method of precise positioning of the plasma needle with respect to the treated tissue.

Stoffels, E.; Kieft, I.E.; Sladek, R.E.J.; Slaaf, D.W.; Laan, E.P. van der; Jimenez-Moreno, P.; Steinbuch, M. [Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven (Netherlands); Department of Mechanical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven (Netherlands)

2004-12-01T23:59:59.000Z

283

Half-lives of Double $\\beta ^+$-decay with Two Neutrinos  

E-Print Network [OSTI]

Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated hal...

Ren, Yuejiao

2015-01-01T23:59:59.000Z

284

"Each and everyone of us can make changes in the way we live our lives and become part of the solution [to climate change]"  

E-Print Network [OSTI]

Green Guide "Id put my money on the sun and solar energy. What a source of power! I hope we dont have of the solution [to climate change]" Al Gore, ,,An Inconvenient Truth BaltimoreGreen Living Guide www.jhsph.edu/green content useful. Cheers, and happy green living! "Be the change you want to see in the world" -- Gandhi

Scharfstein, Daniel

285

Comparison of median frequency between traditional and functional sensor placements during activity monitoring  

E-Print Network [OSTI]

Long-term monitoring is of great clinical relevance. Accelerometers are often used to provide information about activities of daily living. The median frequency (f[subscript m]) of acceleration has recently been suggested ...

Graham, Selina

286

E-Print Network 3.0 - active cascade volcano Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

2 Living with Volcanic Risk in the Cascades U.S. Department of the Interior Summary: ash and rock potentially active volcanoes. Cascade volcanoes tend to erupt explosively, and...

287

Mitigating container security risk using real-time monitoring with active Radio Frequency Identification and sensors  

E-Print Network [OSTI]

The global village in which we live enables increased trade and commerce across regions but also brings a complicated new set of challenges such as terrorist activity, human and drug smuggling and theft in foreign or ...

Schlesinger, Adam Ian

2005-01-01T23:59:59.000Z

288

Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps  

SciTech Connect (OSTI)

Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

none,; Grisham, Larry R.

2014-02-24T23:59:59.000Z

289

Living and Working Safely Around High-Voltage Power Lines.  

SciTech Connect (OSTI)

High-voltage transmission lines can be just as safe as the electrical wiring in the homes--or just as dangerous. The crucial factor is ourselves: they must learn to behave safely around them. This booklet is a basic safety guide for those who live and work around power lines. It deals primarily with nuisance shocks due to induced voltages, and with potential electric shock hazards from contact with high-voltage lines. References on possible long-term biological effects of transmission lines are shown. In preparing this booklet, the Bonneville Power Administration has drawn on more than 50 years of experience with high-voltage transmission. BPA operates one of the world`s largest networks of long-distance, high-voltage lines. This system has more than 400 substations and about 15,000 miles of transmission lines, almost 4,400 miles of which are operated at 500,000 volts.

United States. Bonneville Power Administration.

2001-06-01T23:59:59.000Z

290

Near-Term Climate Mitigation by Short-Lived Forcers  

SciTech Connect (OSTI)

Emissions reductions focused on anthropogenic climate forcing agents with relatively short atmospheric lifetimes such as methane (CH4) and black carbon (BC) have been suggested as a strategy to reduce the rate of climate change over the next several decades. We find that reductions of methane and BC would likely have only a modest impact on near-term climate warming. Even with maximally feasible reductions phased in from 2015 to 2035, global mean temperatures in 2050 are reduced by 0.16 °C, with an uncertainty range of 0.04-0.36°C, with the high end of this range only possible if total historical aerosol forcing is small. More realistic mitigation scenarios would likely provide a smaller climate benefit. The climate benefits from targeted reductions in short-lived forcing agents are smaller than previously estimated and are not substantially different in magnitude from the benefits due to a comprehensive climate policy.

Smith, Steven J.; Mizrahi, Andrew H.

2013-08-12T23:59:59.000Z

291

Microtubule dynamics and chromosome motion visualized in living anaphase cells  

E-Print Network [OSTI]

Abstract. Chromosome segregation in most animal cells is brought about through two events: the movement of the chromosomes to the poles (anaphase A) and the movement of the poles away from each other (anaphase B). Essential to an understanding of the mechanism of mitosis is information on the relative movements of components of the spindle and identification of sites of subunit loss from shortening microtubules. Through use of tubulin derivatized with X-rhodamine, photobleaching, and digital imaging microscopy of living cells, we directly determined the relative movements of poles, chromosomes, and a marked domain on kinetochore fibers during anaphase. During chromosome movement and pole-pole separation, the marked domain did not move significantly

Gary J. Gorbsky; Paul J. Sammak; Gary G. Borisy

1988-01-01T23:59:59.000Z

292

Air Leakage and Air Transfer Between Garage and Living Space  

SciTech Connect (OSTI)

This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

Rudd, A.

2014-09-01T23:59:59.000Z

294

Report on First Activations with the Lead Slowing Down Spectrometer  

SciTech Connect (OSTI)

On Feb. 17 and 18 2011, six items were irradiated with neutrons using the Lead Slowing Down Spectrometer. After irradiation, dose measurements and gamma-spectrometry measurements were completed on all of the samples. No contamination was found on the samples, and all but one provided no dose. Gamma-spectroscopy measurements qualitatively agreed with expectations based on the materials, with the exception of silver. We observed activation in the room in general, mostly due to 56Mn and 24Na. Most of the activation was short lived, with half-lives on the scale of hours, except for 198Au which has a half-life of 2.7 d.

Warren, Glen A.; Mace, Emily K.; Pratt, Sharon L.; Stave, Sean; Woodring, Mitchell L.

2011-03-03T23:59:59.000Z

295

Activated-sludge process: Waste treatment. January 1985-July 1989 (Citations from the Biobusiness data base). Report for January 1985-July 1989  

SciTech Connect (OSTI)

This bibliography contains citations concerning the use of the activated sludge process in waste and waste-water treatment. Biochemistry of the activated sludge process, the effects of various pollutants on process activity, effects of environmental variables such as oxygen and water levels, nutrient requirements of microorganisms employed in activated-sludge processes, and the application of the process to specific wastes such as pharmaceuticals, halocarbons, metallic wastes, dairy wastes, coke-plant waste waters, and petrochemical effluents are among the topics discussed. Operating experiences at large plants are included. (Contains 154 citations fully indexed and including a title list.)

Not Available

1989-08-01T23:59:59.000Z

296

Work and Life in the Balance: Ways of Working and Living Among Elite French, Norwegian, and American Professionals  

E-Print Network [OSTI]

French, Norwegian, and American managers and professionals go about their professional and personal lives. Broad

Schulz, Jeremy Markham

2010-01-01T23:59:59.000Z

297

Sunset magazine : in search of a house for western living  

E-Print Network [OSTI]

This thesis inquires into Sunset's activity as a home magazine in the 1930s and 1940s. In viewing this period, this study draws from Sunset's entire history: from its inception in 1898 as a travel brochure for Southern ...

Gonzalez, Robert Alexander

1993-01-01T23:59:59.000Z

298

Nuclear $\\beta$-decay half-lives in the relativistic point-coupling model  

E-Print Network [OSTI]

The self-consistent proton-neutron quasiparticle random phase approximation approach is employed to calculate $\\beta$-decay half-lives of neutron-rich even-even nuclei with $8\\leqslant Z \\leqslant 30$. A newly proposed nonlinear point-coupling effective interaction PC-PK1 is used in the calculations. It is found that the isoscalar proton-neutron pairing interaction can significantly reduce $\\beta$-decay half-lives. With an isospin-dependent isoscalar proton-neutron pairing strength, our results well reproduce the experimental $\\beta$-decay half-lives, although the pairing strength is not adjusted using the half-lives calculated in this study.

Wang, Z Y; Niu, Y F; Guo, J Y

2015-01-01T23:59:59.000Z

299

Live Webinar on Better Buildings Case Competition: Taking Commercial PACE Financing to Scale, a Case Study  

Broader source: Energy.gov [DOE]

The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

300

Live Webinar on Better Buildings Case Competition: Energy Efficiency in the Restaurant Franchise Model Case Study  

Broader source: Energy.gov [DOE]

The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


301

E-Print Network 3.0 - antibody-positive women living Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

We do not live in an ideal world ... Source: Wynne, Randolph H. - Department of Forestry, Virginia Tech Collection: Environmental Sciences and Ecology 47 ABSTRACT: We assayed...

302

E-Print Network 3.0 - attenuated live infectious Sample Search...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Live, attenuated coccidial vaccines... . Infectious laryngotracheitis (ILT) 6. Visceral gout 7. Fatty liver 8. Cloacal prolapse 9. Osteomalacia 10... Infection (Colibacillosis)...

303

Vanderbilt University Pet Policy for Live-in Employees Vanderbilt University permits live-in employees, be they faculty or staff, to own and keep pets  

E-Print Network [OSTI]

1 Vanderbilt University Pet Policy for Live-in Employees Vanderbilt pets in their University assigned housing. The requirements of the policy are as follows: 1. Pets are limited to domesticated dogs, domesticated cats and fish

Bordenstein, Seth

304

Cellulase producing microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

Dees, H.C.

1997-12-30T23:59:59.000Z

305

Cellulase producing microorganism ATCC 55702  

DOE Patents [OSTI]

Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

Dees, H. Craig (Lenoir City, TN)

1997-01-01T23:59:59.000Z

306

Catalyst activator  

DOE Patents [OSTI]

A catalyst activator particularly adapted for use in the activation of metal complexes of metals of Group 3-10 for polymerization of ethylenically unsaturated polymerizable monomers, especially olefins, comprising two Group 13 metal or metalloid atoms and a ligand structure including at least one bridging group connecting ligands on the two Group 13 metal or metalloid atoms.

McAdon, Mark H. (Midland, MI); Nickias, Peter N. (Midland, MI); Marks, Tobin J. (Evanston, IL); Schwartz, David J. (Lake Jackson, TX)

2001-01-01T23:59:59.000Z

307

Memory Saves Lives: Inter-generational Warnings Effectiveness - 13556  

SciTech Connect (OSTI)

The 2011 Tohoku earthquake and tsunami was a world-class natural disaster. It has been described as the most powerful earthquake ever in Japan, and as one of the most powerful earthquakes ever noted in the world. The toll in terms of human lives lost and property destruction was unimaginable. Even the word 'horrible' is inadequate to describe the suffering and misery that resulted. Nations with nuclear power programs are engaged in, or at least planning to become engaged in, arranging to eventually dispose of their higher-level radioactive waste materials in deep geologic repositories. Geologic repositories are passive safety systems, and if undisturbed isolate these dangerous materials form the biosphere for extremely long times. The key words, however, are 'if undisturbed'. To assure that future generations do not inadvertently drill into repositories, national programs, and the international community (the Records, Knowledge and Memory (RK and M) preservation project of the Nuclear Energy Agency, for example), are proposing to place markers and/or monuments on closed repository sites that say 'do not drill here, and this is why' in various sophisticated ways. Such markers or monuments are attempts at providing passive institutional controls. The effectiveness of messages from past generations to a present generation may give an indication of how effective such passive institutional controls may be. (authors)

Van Luik, Abraham; Patterson, Russell [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States); Shafer, David [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States)] [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States); Klein, Thomas [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)

2013-07-01T23:59:59.000Z

308

Nonequilibrium equation of state in suspensions of active colloids  

E-Print Network [OSTI]

Active colloids constitute a novel class of materials composed of colloidal-scale particles locally converting chemical energy into motility, mimicking micro-organisms. Evolving far from equilibrium, these systems display structural organizations and dynamical properties distinct from thermalized colloidal assemblies. Harvesting the potential of this new class of systems requires the development of a conceptual framework to describe these intrinsically nonequilibrium systems. We use sedimentation experiments to probe the nonequilibrium equation of state of a bidimensional assembly of active Janus microspheres, and conduct computer simulations of a model of self-propelled hard disks. Self-propulsion profoundly affects the equation of state, but these changes can be rationalized using equilibrium concepts. We show that active colloids behave, in the dilute limit, as an ideal gas with an activity-dependent effective temperature. At finite density, increasing the activity is similar to increasing adhesion between equilibrium particles. We quantify this effective adhesion and obtain a unique scaling law relating activity and effective adhesion in both experiments and simulations. Our results provide a new and efficient way to understand the emergence of novel phases of matter in active colloidal suspensions.

Félix Ginot; Isaac Theurkauff; Demian Levis; Christophe Ybert; Lydéric Bocquet; Ludovic Berthier; Cécile Cottin-Bizonne

2014-11-26T23:59:59.000Z

309

Towards a 'Thermodynamics' of Active Matter  

E-Print Network [OSTI]

Self-propulsion allows living systems to display unusual collective behavior. Unlike passive systems in thermal equilibrium, active matter systems are not constrained by conventional thermodynamic laws. A question arises however as to what extent, if any, can concepts from classical thermodynamics be applied to nonequilibrium systems like active matter. Here we use the new swim pressure perspective to develop a simple theory for predicting phase separation in active matter. Using purely mechanical arguments we generate a phase diagram with a spinodal and critical point, and define a nonequilibrium chemical potential to interpret the "binodal." We provide a generalization of thermodynamic concepts like the free energy and temperature for nonequilibrium active systems. Our theory agrees with existing simulation data both qualitatively and quantitatively and may provide a framework for understanding and predicting the behavior of nonequilibrium active systems.

Sho C. Takatori; John F. Brady

2014-11-21T23:59:59.000Z

310

Human Activity Detection from RGBD Images  

E-Print Network [OSTI]

Being able to detect and recognize human activities is important for making personal assistant robots useful in performing assistive tasks. The challenge is to develop a system that is low-cost, reliable in unstructured home settings, and also straightforward to use. In this paper, we use a RGBD sensor (Microsoft Kinect) as the input sensor, and present learning algorithms to infer the activities. Our algorithm is based on a hierarchical maximum entropy Markov model (MEMM). It considers a person's activity as composed of a set of sub-activities, and infers the two-layered graph structure using a dynamic programming approach. We test our algorithm on detecting and recognizing twelve different activities performed by four people in different environments, such as a kitchen, a living room, an office, etc., and achieve an average performance of 84.3% when the person was seen before in the training set (and 64.2% when the person was not seen before).

Sung, Jaeyong; Selman, Bart; Saxena, Ashutosh

2011-01-01T23:59:59.000Z

311

Chemical behavior of long-lived radionuclides in the marine environment  

SciTech Connect (OSTI)

Measurements of long-lived radionuclides in the marine environment have provided a wealth of information regarding the physical, biological, and chemical processes which control the behavior of these and many other pollutants in the oceans. Their value as tracers for the dispersion, transport, and fate of pollutants in the oceans is largely dependent on the chemical properties of each individual radioelement. Differences in these properties, particularly in relation to their interaction with biotic or abiotic particulate matter, result in the separation of parent-daughter radioisotopes in the natural radioelement series or in changes in the ratios of fission and activation products. Such differences have provided the means to provide time scales for a variey of transport processes and to determine sedimentation rates. The properties of these radionuclides in the oceans can, in general, be predicted from the chemical properties of the stable elements. For those elements such as plutonium, for which there are no naturally-occurring stable isotopes, studies of their distribution in the oceans have provided a new important understanding of their chemical behavior. This behavior has not always agreed with what would have been predicted from laboratory studies carried out at far higher concentrations. Differences between observed distributions and laboratory predictions have highlighted the importance of correct experimental conditions in order to avoid confusing experimental artifacts. The interaction of radionuclides with particles in the oceans and marine sediments can be described in terms of simple ion exchange or adsorption equilibria.

Edgington, D.N.; Nelson, D.M.

1983-01-01T23:59:59.000Z

312

Dissecting Regional Variations in Stress Fiber Mechanics in Living Cells with Laser Nanosurgery  

SciTech Connect (OSTI)

The ability of a cell to distribute contractile stresses across the extracellular matrix in a spatially heterogeneous fashion underlies many cellular behaviors, including motility and tissue assembly. Here we investigate the biophysical basis of this phenomenon by using femtosecond laser nanosurgery to measure the viscoelastic recoil and cell-shape contributions of contractile stress fibers (SFs) located in specific compartments of living cells. Upon photodisruption and recoil, myosin light chain kinase-dependent SFs located along the cell periphery display much lower effective elasticities and higher plateau retraction distances than Rho-associated kinase-dependent SFs located in the cell center, with severing of peripheral fibers uniquely triggering a dramatic contraction of the entire cell within minutes of fiber irradiation. Image correlation spectroscopy reveals that when one population of SFs is pharmacologically dissipated, actin density flows toward the other population. Furthermore, dissipation of peripheral fibers reduces the elasticity and increases the plateau retraction distance of central fibers, and severing central fibers under these conditions triggers cellular contraction. Together, these findings show that SFs regulated by different myosin activators exhibit different mechanical properties and cell shape contributions. They also suggest that some fibers can absorb components and assume mechanical roles of other fibers to stabilize cell shape.

Tanner, Kandice; Boudreau, Aaron; Bissell, Mina J; Kumar, Sanjay

2010-03-02T23:59:59.000Z

313

www.hfhl.umn.edu Healthy Foods, Healthy Lives (HFHL) Institute  

E-Print Network [OSTI]

Lives (HFHL) Institute is an all-university Institute supported by funds from the University funding cycle (fall 2014) OR proposals may be prepared and submitted to other equivalent Universitywww.hfhl.umn.edu Healthy Foods, Healthy Lives (HFHL) Institute Planning Grant Program for Community-University

Amin, S. Massoud

314

Safety, Liveness and Fairness in Temporal Logic \\Lambda A. Prasad Sistla  

E-Print Network [OSTI]

Safety, Liveness and Fairness in Temporal Logic \\Lambda A. Prasad Sistla Department of Electrical for proving correctness with respect to these properties. The properties that we consider include safety Logic, Safety Properties, Live­ ness Properties, Fairness Properties, Algorithms. \\Lambda This work

Sistla, A. Prasad

315

Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer  

E-Print Network [OSTI]

Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer University of Colorado as tools, particularly computational artifacts. We introduce the notions of tools for living and tools for the design of artifacts. The tools concept is then studied in the context of the Memory Aiding Prompting

Fischer, Gerhard

316

How did LUCA make a living? Chemiosmosis in the origin of life  

E-Print Network [OSTI]

necessary for carbon and energy metabolism in all free-living chemotrophs, and presum- ably the first free-living cells too. Proton gradients form naturally at alkaline hydrothermal vents and are viewed as central was hydrogen and the first acceptor CO2. Keywords: alkaline hydrothermal vents; ATPase; chemi- osmosis; LUCA

Allen, John F.

317

Kinetics of `living' radical polymerizations of multifunctional monomers , A. Shahar2  

E-Print Network [OSTI]

Kinetics of `living' radical polymerizations of multifunctional monomers J.H. Ward1 , A. Shahar2 2001; accepted 16 November 2001 Abstract The polymerizations of multifunctional monomers photocalorimetry. The iniferter was used to simulate a `living' radical polymerization. The normal autoacceleration

Peppas, Nicholas A.

318

Postdoctoral Positions In-vivo Optical Imaging and Microscopy of the Living Brain  

E-Print Network [OSTI]

Postdoctoral Positions In-vivo Optical Imaging and Microscopy of the Living Brain Columbia insight into the function and physiology of the living brain. We are particularly interested in exploring brain. Neurovascular coupling is important both because it is the basis of the fMRI BOLD signal

Adams, Mark

319

Neutron-induced cross sections of short-lived nuclei via the surrogate reaction method  

E-Print Network [OSTI]

Neutron-induced cross sections of short-lived nuclei via the surrogate reaction method G. Boutoux1 Abstract. The measurement of neutron-induced cross sections of short-lived nuclei is extremely difficult for a neutron-induced measurement. We have successfully used the surrogate reaction method to extract neutron

Boyer, Edmond

320

Greening of Appalachia 59 hose of us who live in Appalachia are blessed  

E-Print Network [OSTI]

quickly recognize the visible outcomes of damaged ecosystems: declining forests, reduced water qualityGreening of Appalachia 59 T hose of us who live in Appalachia are blessed with living in an area of stunning environmental beauty and diversity. Some of the most unique ecosystems in the world exist here

Karsai, Istvan

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


321

Reproductive Life Events in the Population Living in the Vicinity of a Nuclear Waste Reprocessing Plant  

E-Print Network [OSTI]

: There is concern about the health of populations living close to nuclear waste reprocessing plants. We conducted the health of the population living in the vicinity of nuclear waste reprocessing plants was raised the Dounreay nuclear waste reprocessing plant (United Kingdom).[1] Similar studies around the French nuclear

Paris-Sud XI, Université de

322

Baccalaureate and Beyond: A First Look at the Employment Experiences and Lives  

E-Print Network [OSTI]

Baccalaureate and Beyond: A First Look at the Employment Experiences and Lives of College Graduates blank. #12;Baccalaureate and Beyond: A First Look at the Employment Experiences and Lives of College Jennifer Cooney RTI International Ted Socha Project Officer National Center for Education Statistics NCES

Rohs, Remo

323

Towards Living Inter-Organizational Processes Ruth Breu, Schahram Dustdar, Johann Eder, Christian Huemer, Gerti Kappel,  

E-Print Network [OSTI]

Towards Living Inter-Organizational Processes Ruth Breu, Schahram Dustdar, Johann Eder, Christian in practice for enabling organizations to increase their effectiveness, efficiency, and flexibility processes, which we refer to as living inter-organizational processes. Such processes are not in control

Dustdar, Schahram

324

Mapping the ECG in the live rabbit heart using Ultrasound Current Source Density  

E-Print Network [OSTI]

Mapping the ECG in the live rabbit heart using Ultrasound Current Source Density Imaging with coded for mapping the electrocardiogram (ECG) in a live rabbit heart preparation. Results confirmed that chirps im demonstrated mapping the ECG using a clinical intracardiac catheter, 1 MHz ultrasound transducer and coded

Witte, Russell S.

325

the Difference 1 the DifferenceYOUR GIVING CHANGES LIVES  

E-Print Network [OSTI]

societies, allowing more students to participate in theatre and performance activities. Hydrogen train Studentship Burkitt's Lymphoma Students and young researchers at Birmingham built the world's first hydrogen locomotive engine. The train used cutting-edge propulsion technology and was entered in the first Institution

Birmingham, University of

326

MassLive.com's Printer-Friendly Page http://www.masslive.com/printer/printer.ssf?/base/living-0/11284120... 1 of 1 10/7/2005 12:47 PM  

E-Print Network [OSTI]

nanowires, which are electrically conductive. The hope is Geobacter could be engineered to harvest a $21.8 million grant to continue his investigation into microbes that can harvest electricity from the microorganism Geobacter, specifically which of Geobacter's genes do what and in which environment

Lovley, Derek

327

Systematic Law for Half-lives of Double $?$-decay with Two Neutrinos  

E-Print Network [OSTI]

Nuclear double $\\beta$-decay with two neutrinos is a rare and important process for natural radioactivity of unstable nuclei. The experimental data of nuclear double $\\beta^{-}$-decay with two neutrinos are analyzed and a systematic law to calculate the half-lives of this rare process is proposed. It is the first analytical and simple formula for double $\\beta$-decay half-lives where the leading effect from both the Coulomb potential and nuclear structure is included. The systematic law shows that the logarithms of the half-lives are inversely proportional to the decay energies for the ground state transitions between parent nuclei and daughter nuclei. The calculated half-lives are in agreement with the experimental data of all known eleven nuclei with an average factor of 3.06. The half-lives of other possible double $\\beta$-decay candidates with two neutrinos are predicted and these can be useful for future experiments. The law, without introducing any extra adjustment, is also generalized to the calculations of double $\\beta $-decay half-lives from the ground states of parent nuclei to the first $0^+$ excited states of daughter nuclei and the calculated half-lives agree very well with the available data. Some calculated half-lives are the first theoretical results of double $\\beta $-decay half-lives from the ground states of parent nuclei to the first $0^+$ excited states of daughter nuclei. The similarity and difference between the law of $\\alpha$-decay and that of double $\\beta^{-}$-decay are also analyzed and discussed.

Yuejiao Ren; Zhongzhou Ren

2014-06-04T23:59:59.000Z

328

Search for long lived heaviest nuclei beyond the valley of stability  

E-Print Network [OSTI]

The existence of long lived superheavy nuclei (SHN) is controlled mainly by spontaneous fission and $\\alpha$-decay processes. According to microscopic nuclear theory, spherical shell effects at Z=114, 120, 126 and N=184 provide the extra stability to such SHN to have long enough lifetime to be observed. To investigate whether the so-called "stability island" could really exist around the above Z, N values, the $\\alpha$-decay half lives along with the spontaneous fission and $\\beta$-decay half lives of such nuclei are studied. The $\\alpha$-decay half lives of SHN with Z=102-120 are calculated in a quantum tunneling model with DDM3Y effective nuclear interaction using $Q_\\alpha$ values from three different mass formulae prescribed by Koura, Uno, Tachibana, Yamada (KUTY), Myers, Swiatecki (MS) and Muntian, Hofmann, Patyk, Sobiczewski (MMM). Calculation of spontaneous fission (SF) half lives for the same SHN are carried out using a phenomenological formula and compared with SF half lives predicted by Smolanczuk {\\it et al}. Possible source of discrepancy between the calculated $\\alpha$-decay half lives of some nuclei and the experimental data of GSI, JINR-FLNR, RIKEN are discussed. In the region of Z=106-108 with N$\\sim$ 160-164, the $\\beta$-stable SHN $^{268}_{106}Sg_{162}$ is predicted to have highest $\\alpha$-decay half life ($T_\\alpha \\sim 3.2hrs$) using $Q_\\alpha$ value from MMM. Interestingly, it is much greater than the recently measured $T_\\alpha$ ($\\sim 22s$) of deformed doubly magic $^{270}_{108}Hs_{162}$ nucleus. A few fission-survived long-lived SHN which are either $\\beta$-stable or having large $\\beta$-decay half lives are predicted to exist near $^{294}110_{184}$, $^{293}110_{183}$, $^{296}112_{184}$ and $^{298}114_{184}$. These nuclei might decay predominantly through $\\alpha$-particle emission.

P. Roy Chowdhury; C. Samanta; D. N. Basu

2008-02-26T23:59:59.000Z

329

9/4/12 Tigers change behavior to live near people | TG Daily 1/9www.tgdaily.com/sustainability-features/65902-tigers-change-behavior-to-live-near-people  

E-Print Network [OSTI]

9/4/12 Tigers change behavior to live near people | TG Daily 1/9www.tgdaily.com/sustainability change behavior to live near people | TG Daily 2/9www.tgdaily.com/sustainability-features/65902-tigers-change-behavior-to-live people tigers Advertisement #12;9/4/12 Tigers change behavior to live near people | TG Daily 3/9www.tgdaily.com/sustainability

330

Existence of long-lived isomeric states in naturally-occurring neutron-deficient Th isotopes  

SciTech Connect (OSTI)

Four long-lived neutron-deficient Th isotopes with atomic mass numbers 211 to 218 and abundances of (1-10)x10{sup -11} relative to {sup 232}Th have been found in a study of naturally-occurring Th using inductively coupled plasma-sector field mass spectrometry. It is deduced that long-lived isomeric states exist in these isotopes. The hypothesis that they might belong to a new class of long-lived high spin super- and hyperdeformed isomeric states is discussed.

Marinov, A.; Kashiv, Y. [Racah Institute of Physics, Hebrew University of Jerusalem, Jerusalem 91904 (Israel); Rodushkin, I. [Analytica AB, Aurorum 10, S-977 75 Luleaa (Sweden); Halicz, L.; Segal, I. [Geological Survey of Israel, 30 Malkhei Israel St., Jerusalem 95501 (Israel); Pape, A. [IPHC-UMR7178, IN2P3-CNRS/ULP, BP 28, F-67037 Strasbourg cedex 2 (France); Gentry, R. V. [Earth Science Associates, P.O. Box 12067, Knoxville, Tennessee 37912-0067 (United States); Miller, H. W. [P. O. Box 1092, Boulder, Colorado 80306-1092 (United States); Kolb, D. [Department of Physics, University GH Kassel, D-34109 Kassel (Germany); Brandt, R. [Kernchemie, Philipps University, D-35041 Marburg (Germany)

2007-08-15T23:59:59.000Z

331

Vegetation response to burning thicketized live oak savannah on the Aransas National Wildlife Refuge  

E-Print Network [OSTI]

of live oak stems by height class in February, April, and July of 1976 after burning on several dates on the Aransas National Wildife Refuge near Austwell, Texas, ~ . . . ~ . . . ~ ~ ~ 48 15 Canopy cover (/) of live oak, grass, and f orbs in February... 1977 on areas burned at various dates on the Aransas National Wildlife Refuge near Austwell, Texas. . 49 16 Total Standing crop and new growth (kg/ha) of live oak and standing crop increase (/) represented by new growth in April and July, 1976...

Kelley, David Mitchell

1980-01-01T23:59:59.000Z

332

The use of ultrasonic techniques in estimating carcass traits in live beef animals  

E-Print Network [OSTI]

on ultrasonic ribeye area with live weight and fat adjusted, a hierarchal (nested) analysis of variance was set up which consisted of: Sources of Variation d. f. Total Breed Sex/breed Sire/sex/breed Error 391 3 5 80 303 In addition, for determining... ribeye area, ultrasonic fat depth and actual fat depth. 2. Age in days, live weight, ultrasonic ribeye area, ultra- sonic fat depth, actual ribeye area and actual fat depth. 3. Live weight, ultrasonic ribeye area and ultrasonic fat depth. 4. Age...

Wideman, Donald

2012-06-07T23:59:59.000Z

333

Activity report  

SciTech Connect (OSTI)

This report is aimed to show the author's activities to support the LDRD. The title is 'Investigation of the Double-C Behavior in the Pu-Ga Time-Temperature-Transformation Diagram' The sections are: (1) Sample Holder Test; (2) Calculation of x-ray diffraction patterns; (3) Literature search and preparing publications; (4) Tasks Required for APS Experiments; and (5) Communications.

Yu, S W

2008-08-11T23:59:59.000Z

334

Design for the Frail Old: Environmental and Perceptual Influences on Corridor Walking Behaviors of Assisted Living Residents  

E-Print Network [OSTI]

living among these residents and yet, there is little research that has been done in this regard. The researcher conducted two studies in Central Texas to explore how corridor design features influenced indoor walking behaviors among assisted living...

Lu, Zhipeng

2010-10-12T23:59:59.000Z

335

Forging an Ascetic Planet: Jesuit Lives and Virtues on the Mission Frontier of Eighteenth-Century New Spain  

E-Print Network [OSTI]

Angeles Forging an Ascetic Planet: Jesuit Lives and VirtuesForging an Ascetic Planet: Jesuit Lives and Virtues on thebe a thoroughly “ascetic planet,” by which he meant that a

Green, Bryan David

2012-01-01T23:59:59.000Z

336

An estimated one of every six households (16.2 percent) in Texas lives in poverty. Research has  

E-Print Network [OSTI]

Relevance An estimated one of every six households (16.2 percent) in Texas lives in poverty. Research has shown that individuals who live in poverty have dietary intakes that are not in agreement

337

Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site  

SciTech Connect (OSTI)

Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the “fines” fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

2011-02-15T23:59:59.000Z

338

Fluctuations and Rheology in Active Bacterial Suspensions D. T. N. Chen,1  

E-Print Network [OSTI]

the power spectrum of the active stress fluctuations. In some cases, we observe 1= ! p scaling in the noise Active complex fluid systems such as living cells [1,2], assemblies of motors and filaments [3], flocks of birds [4], and vibrated granular media [5] differ from conventional equilibrium media in that some

McGaughey, Alan

339

Volume 6, Number 2 June 2009 Cost Of Living: How Does Morgantown Compare In The First Quarter  

E-Print Network [OSTI]

Volume 6, Number 2 June 2009 Cost Of Living: How Does Morgantown Compare In The First Quarter Of 2009? By Amy Higginbotham For the first quarter of 2009, Morgantown's cost of living was average according to the ACCRA Cost of Living Index, which compared prices of sixty specific items in 309 urban

Mohaghegh, Shahab

340

LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Care Daily Checklist.docx revised 8/9/13 Page 1 of 1  

E-Print Network [OSTI]

LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Care Daily Checklist.docx revised 8/9/13 Page 1 of 1 Live Fish Care Daily Checklist D. Clark Labs rooms G-06 & G-08 morning visit: Turn) eggs from fridge in room 382; feed the live fish there Feed hatched Artemia (=brine shrimp) to fry

Langerhans, Brian

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


341

Wash your hands thoroughly with soap and water right after touching live poultry or anything in the  

E-Print Network [OSTI]

CS222453 DO: · Wash your hands thoroughly with soap and water right after touching live poultry poultry outside the house, such as cages or feed or water containers. Contact with live poultry (chicks. · Chicks, ducklings, and other live poultry can carry Salmonella germs and still appear healthy and clean

New Hampshire, University of

342

Live Webinar on the Funding Opportunity for Marine and Hydrokinetic Research and Development University Consortium  

Broader source: Energy.gov [DOE]

On April 24, 2014 from 1:00 - 2:30 PM EDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Marine and Hydrokinetic (MHK) Research and...

343

Better Living with xlinkit Christian Nentwich, Wolfgang Emmerich and Anthony Finkelstein  

E-Print Network [OSTI]

Better Living with xlinkit Christian Nentwich, Wolfgang Emmerich and Anthony Finkelstein Department of Computer Science University College London, London WC1E 6BT {c.nentwich,w.emmerich

Finkelstein, Anthony

344

Live Webcast on the 2014 Farm Bill's Renewable Energy for America Program  

Broader source: Energy.gov [DOE]

The Energy Department will present a live webcast titled “The 2014 Farm Bill's Renewable Energy for America Program” on May 21, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

345

Optimized Live Heap Bound Analysis Leena Unnikrishnan Scott D. Stoller Yanhong A. Liu ?  

E-Print Network [OSTI]

Optimized Live Heap Bound Analysis Leena Unnikrishnan #3; Scott D. Stoller #3; Yanhong A. Liu algorithm is useful for evaluating other replacement algorithms. The analysis can easily be modi#12;ed

Liu, Yanhong Annie

346

M49-- Machine for the Living : a performance broadcast through an interfering FM radio transmission  

E-Print Network [OSTI]

The focus of the M49: Machine For The Living project is a performance work that considers the omnipresence of layered communications, which extend, yet supersede corporeal space of the individual. M49 creates a framework ...

Goldfarb, Maximilian M

2006-01-01T23:59:59.000Z

347

Sipping fuel and saving lives: increasing fuel economy without sacrificing safety  

E-Print Network [OSTI]

Ford delays plans to boost fuel economy of its SUVs. WallImproving safety without impacting fuel economy. Honda MotorCompany, October 4. SIPPING FUEL AND SAVING LIVES / 24

Gordon, Deborah; Greene, David L.; Ross, Marc H.; Wenzel, Tom P.

2008-01-01T23:59:59.000Z

348

Live Webinar on the Funding Opportunity for Wind Forecasting Improvement Project in Complex Terrain  

Broader source: Energy.gov [DOE]

On April 21, 2014 from 3:00 to 5:00 PM EST the Wind Program will hold a live webinar to provide information to potential applicants for this Funding Opportunity Announcement. There is no cost to...

349

New reporters of protein trafficking and protein-protein interactions in live cells  

E-Print Network [OSTI]

Here, we describe our attempts to harness the exquisite specificity of natural protein and RNA enzymes to develop improved methods to study protein localization and protein-protein interactions in live cells. We first ...

Fernández Suárez, Marta

2008-01-01T23:59:59.000Z

350

Camera that takes pictures of aircraft and ground vehicle tires can save lives  

E-Print Network [OSTI]

;Camera that takes pictures of aircraft and ground vehicle tires can save lives Yair Wiseman Holon Institute of Technology Computer Science Department Holon 58102, Israel E-mail: wiseman

Wiseman, Yair

351

Working lives of prison managers: exploring agency and structure in the late modern prison   

E-Print Network [OSTI]

This study explores the contemporary working lives of prison managers. It attempts to understand the ways in which globalised changes in management practices have intersected with localised practices and occupational ...

Bennett, Jamie Stewart

2012-11-27T23:59:59.000Z

352

Making lives under closure : birth and medicine in Palestine's waiting zones  

E-Print Network [OSTI]

Reproduction is a site for understanding the ways in which people reconceptualize and re-organize the world in which they live. This dissertation tries to understand the world of birth under the regime of closures and ...

Wick, Livia

2006-01-01T23:59:59.000Z

353

Use of encapsulated live microalgae to investigate pre-ingestive selection in the oyster Crassostrea gigas  

E-Print Network [OSTI]

Use of encapsulated live microalgae to investigate pre-ingestive selection in the oyster, this study clearly demonstrate that extracellular metabolites produced by microalgae play a crucial role reserved. Keywords: Alginate; Beads; Bivalve; Feeding; Microalgae; Particle processing 1. Introduction

Allam, Bassem

354

Live Webinar on the Funding Opportunity for Technology Incubator for Wind Energy Innovations  

Broader source: Energy.gov [DOE]

On April 17, 2014, from 1:00 – 3:00 PM MDT, the Wind Program will hold a live webinar to provide information to potential applicants for the Technology Incubator for Wind Energy Innovations Funding Opportunity.

355

E-Print Network 3.0 - advanced cancer living Sample Search Results  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Powered by Explorit Topic List Advanced Search Sample search results for: advanced cancer living Page: << < 1 2 3 4 5 > >> 1 Learn more about our programs at: Visit us at our...

356

U.S. Bamboo house of the future : standardizing ecological living  

E-Print Network [OSTI]

This thesis focuses on ecological living through the use of bamboo. It explores how the material can be used for methods of prefabricated housing design within the United States. It also uses a "ht of parts" and describes ...

Wong, Lucy Lai

2006-01-01T23:59:59.000Z

357

Sustainability processes in community-level health initiatives: the experiences of Scottish healthy living centres   

E-Print Network [OSTI]

Background This thesis explores processes involved in stakeholders’ attempts to secure sustainability of three short-term funded community health initiatives known as healthy living centres (HLCs). The overall aim was ...

Rankin, David

2010-01-01T23:59:59.000Z

358

Federal Biomass Activities  

Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

and Budget Federal Biomass Activities Federal Biomass Activities Biopower Biopower Biofuels Biofuels Bioproducts Bioproducts Federal Biomass Activities Federal Biomass...

359

Report on Second Activations with the Lead Slowing Down Spectrometer  

SciTech Connect (OSTI)

Summary On August 18 and 19 2011, five items were irradiated with neutrons using the Lead Slowing Down Spectrometer (LSDS). After irradiation, dose measurements and gamma-spectrometry measurements were completed on all of the samples. No contamination was found on the samples, and all but one provided no dose. Gamma-spectroscopy measurements qualitatively agreed with expectations based on the materials. As during the first activation run, we observed activation in the room in general, mostly due to 56Mn and 24Na. Most of the activation of the samples was short lived, with half-lives on the scale of hours to days, except for 60Co which has a half-life of 5.3 y.

Stave, Sean C.; Mace, Emily K.; Pratt, Sharon L.; Warren, Glen A.

2012-04-27T23:59:59.000Z

360

On Altruists and Egoists in Activity Participation and Travel: Who are they and do they live together?  

E-Print Network [OSTI]

Travel Behaviour Research, Lucerne, August 2003. Scott, D.M.Proc. 10 th IATRB Conf. , Lucerne, Switzerland (CD-Rom).

Goulias, Konstadinos G.; Henson, Kriste M.

2006-01-01T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


361

A Biocompatible in Vivo Ligation Reaction and Its Application for Noninvasive Bioluminescent Imaging of Protease Activity in Living  

E-Print Network [OSTI]

-7 Staudinger ligation,8-15 alkene-tetrazine reac- tions,16-21 and recently reported Pictet-Spengler ligation.22

Bogyo, Matthew

362

nAture methods | VOL.10 NO.2 | FEBRUARY2013 | 147 measurement of protease activity in living cells or organisms  

E-Print Network [OSTI]

, such as gout6, atherosclerosis7, type 2 diabetes8 and Alzheimer's disease9, engagement of the NLRP3­ASC

Cai, Long

363

Fishing activity, health characteristics and mercury exposure of Amerindian women living alongside the Beni River (Amazonian Bolivia)  

E-Print Network [OSTI]

the Beni River (Amazonian Bolivia) Authors: Eric Benefice 1 ; Selma Luna - Monrroy 1, 2 Ronald Lopez de Ciencias Bioquímicas y Farmacéuticas, Universidad Mayor de San Andrés, La Paz, Bolivia) 3 INLASA, (Instituto Nacional de Laboratorios en Salud, Ministerio de Salud y Deportes), La Paz (Bolivia) Corresponding

Paris-Sud XI, Université de

364

Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° north using ¹³C DNA stable isotope probing and catalyzed activated reporter deposition-fluorescence in situ hybridization  

E-Print Network [OSTI]

At deep-sea hydrothermal vents chemolithoautotrophic microbes mediate the transfer of geothermal chemical energy to higher trophic levels. To better understand these underlying processes and the organisms catalyzing them, ...

Richberg, Kevin Patrick

2010-01-01T23:59:59.000Z

365

SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS  

SciTech Connect (OSTI)

Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. The bell-and-spigot joints tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple cast-iron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs while the pipe remains in service by traveling through the pipe, cleaning each joint surface, and attaching a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, avoid traffic disruption, and eliminate any requirement to interrupt service (which results in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct repair operations on live mains. The development effort is divided into eleven tasks. Task 1-Program Management was previously completed. Two reports, one describing the program management plan and the other consisting of the technology assessment, were submitted to the DOE COR in the first quarter. Task 2-Establishment of Detailed Design Specifications and Task 3-Design and Fabricate Ratcheting Stainless-Steel Repair Sleeves are now well underway. First-quarter activities included conducting detailed analyses to determine the capabilities of coiled-tubing locomotion for entering and repairing gas mains and the first design iteration of the joint-sealing sleeve. The maximum horizontal reach of coiled tubing inside a pipeline before buckling prevents further access was calculated for a wide range of coiled-tubing string designs and pipe environments. Work conducted in the second quarter consisted of: (1) selecting a preferred pan/zoom/tilt camera; (2) initiating design of the digital control electronics and switching power supply for the control and operation of the in-pipe robotic modules; (3) continuing design of the repair sleeve and (4) initial testing of the wall-cleaning device. Activities in the third quarter included: (1) development of the system's pan/zoom/tilt camera control electronics and operating software, and implementing these in the surface and downhole modules and (2) further testing of the wall-cleaning elements used to clean the inside of the bell and spigot joints. Most recently, fourth quarter developments were centered on designing and testing the pipe-wall cleaning device including the selection of the drive motor and its control electronics. In addition, efforts were also focused on the design of the repair sleeve. Details of these activities are described in the body of the report along with a summary of events scheduled for the next quarter.

Kiran M. Kothari; Gerard T. Pittard

2003-06-01T23:59:59.000Z

366

SEALING LARGE-DIAMETER CAST-IRON PIPE JOINTS UNDER LIVE CONDITIONS  

SciTech Connect (OSTI)

Utilities in the U.S. operate over 75,000 km (47,000 miles) of old cast-iron pipes for gas distribution. The bell-and-spigot joints tend to leak as these pipes age. Current repair practices are costly and highly disruptive. The objective of this program is to design, test and commercialize a robotic system capable of sealing multiple cast-iron bell and spigot joints from a single pipe entry point. The proposed system will perform repairs while the pipe remains in service by traveling through the pipe, cleaning each joint surface, and attaching a stainless-steel sleeve lined with an epoxy-impregnated felt across the joint. This approach will save considerable time and labor, avoid traffic disruption, and eliminate any requirement to interrupt service (which results in enormous expense to utilities). Technical challenges include: (1) repair sleeves must compensate for diametric variation and eccentricity of cast-iron pipes; (2) the assembly must travel long distances through pipes containing debris; (3) the pipe wall must be effectively cleaned in the immediate area of the joint to assure good bonding of the sleeve; and (4) an innovative bolt-on entry fitting is required to conduct repair operations on live mains. The development effort is divided into eleven tasks. Task 1--Program Management was previously completed. Two reports, one describing the program management plan and the other consisting of the technology assessment, were submitted to the DOE COR in the first quarter. Task 2--Establishment of Detailed Design Specifications and Task 3--Design and Fabricate Ratcheting Stainless-Steel Repair Sleeves are now well underway. First-quarter activities included conducting detailed analyses to determine the capabilities of coiled-tubing locomotion for entering and repairing gas mains and the first design iteration of the joint-sealing sleeve. The maximum horizontal reach of coiled tubing inside a pipeline before buckling prevents further access was calculated for a wide range of coiled-tubing string designs and pipe environments. Work conducted in the second quarter consisted of: (1) selecting a preferred pan/zoom/tilt camera; (2) initiating design of the digital control electronics and switching power supply for the control and operation of the in-pipe robotic modules; (3) continuing design of the repair sleeve and (4) initial testing of the wall-cleaning device. Most recently, activities in the third quarter included: (1) development of the system's pan/zoom/tilt camera control electronics and operating software, and implementing these in the surface and downhole modules and (2) further testing of the wall-cleaning elements used to clean the inside of the bell and spigot joints. Details of these activities are described in the body of the report along with a summary of events scheduled for the fourth quarter.

Kiran M. Kothari; Gerard T. Pittard

2003-01-01T23:59:59.000Z

367

Respiratory survey of North American Indian children living in proximity to an aluminum smelter  

SciTech Connect (OSTI)

We explored the relationship of respiratory symptoms and lung function to exposure to ambient air pollution consisting of particulate and gaseous fluorides. The subjects were 253 North American Indian children 11 to 17 yr of age living on the Akwasasne reserve, which is adjacent to an aluminum smelter. Among boys, closing volume (CV/VC%) was increased in those raised closest to the smelter as opposed to those having lived most of their lives farthest from this source of air pollution. In both sexes, there was a significant linear relationship between increasing CV/VC% and the amount of fluoride contained in a spot urine sample. We conclude that exposure to fluoride air pollution in the community may be associated with abnormalities in small airways. The implication of these abnormalities for future respiratory health is unknown.

Ernst, P.; Thomas, D.; Becklake, M.R.

1986-02-01T23:59:59.000Z

368

Experimental Measurements of Short-Lived Fission Products from Uranium, Neptunium, Plutonium and Americium  

SciTech Connect (OSTI)

Fission yields are especially well characterized for long-lived fission products. Modeling techniques incorporate numerous assumptions and can be used to deduce information about the distribution of short-lived fission products. This work is an attempt to gather experimental (model-independent) data on the short-lived fission products. Fissile isotopes of uranium, neptunium, plutonium and americium were irradiated under pulse conditions at the Washington State University 1 MW TRIGA reactor to achieve ~108 fissions. The samples were placed on a HPGe (high purity germanium) detector to begin counting in less than 3 minutes post irradiation. The samples were counted for various time intervals ranging from 5 minutes to 1 hour. The data was then analyzed to determine which radionuclides could be quantified and compared to the published fission yield data.

Metz, Lori A.; Payne, Rosara F.; Friese, Judah I.; Greenwood, Lawrence R.; Kephart, Jeremy D.; Pierson, Bruce D.

2009-11-01T23:59:59.000Z

369

Primordial Black Holes as Heat Sources for Living Systems with Longest Possible Lifetimes  

E-Print Network [OSTI]

Just forty years ago, Hawking wrote his famous paper on primordial black holes (PBH). There have been since innumerable discussions on the consequences of the existence of such exotic objects and ramifications of their properties. Here we suggest that PBH's in an ever expanding universe (as implied by dark energy domination, especially of a cosmological constant) could be the ultimate repository for long lived living systems. PBH's having solar surface temperatures would last 10^32 years as a steady power source and should be considered in any discussion on exobiological life.

C Sivaram; Kenath Arun; Kiren O V

2014-02-14T23:59:59.000Z

370

The potential use of thiabendazole and propiconazole for oak wilt control in live oak  

E-Print Network [OSTI]

the bioindicator, following in3ectron of live oaks in July, 1986 and June, 1987 at Round Rock, Texas. 22 Percent of branch samples exhibiting de- tectable amounts of thiabendazole, based b' assay pr d 'ng P. ~ as the bioindicator, following in3ectron of live... separate dates (June, July, September, and December 1986) for a total of 32 treated trees. All trees were reinjected at the same rate in June 1987. A bioassay of twigs, using a fungicide-sensitive Penicillium expansum Link isolate, showed the best...

Roberts, Paul Edwin

1988-01-01T23:59:59.000Z

371

Molecular Imaging of the ATM Kinase Activity  

SciTech Connect (OSTI)

Purpose: Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including from DNA double-strand breaks. ATM activation results in the initiation of a complex cascade of events including DNA damage repair, cell cycle checkpoint control, and survival. We sought to create a bioluminescent reporter that dynamically and noninvasively measures ATM kinase activity in living cells and subjects. Methods and Materials: Using the split luciferase technology, we constructed a hybrid cDNA, ATM-reporter (ATMR), coding for a protein that quantitatively reports on changes in ATM kinase activity through changes in bioluminescence. Results: Treatment of ATMR-expressing cells with ATM inhibitors resulted in a dose-dependent increase in bioluminescence activity. In contrast, induction of ATM kinase activity upon irradiation resulted in a decrease in reporter activity that correlated with ATM and Chk2 activation by immunoblotting in a time-dependent fashion. Nuclear targeting improved ATMR sensitivity to both ATM inhibitors and radiation, whereas a mutant ATMR (lacking the target phosphorylation site) displayed a muted response. Treatment with ATM inhibitors and small interfering (si)RNA-targeted knockdown of ATM confirm the specificity of the reporter. Using reporter expressing xenografted tumors demonstrated the ability of ATMR to report in ATM activity in mouse models that correlated in a time-dependent fashion with changes in Chk2 activity. Conclusions: We describe the development and validation of a novel, specific, noninvasive bioluminescent reporter that enables monitoring of ATM activity in real time, in vitro and in vivo. Potential applications of this reporter include the identification and development of novel ATM inhibitors or ATM-interacting partners through high-throughput screens and in vivo pharmacokinetic/pharmacodynamic studies of ATM inhibitors in preclinical models.

Williams, Terence M. [Department of Radiation Oncology, Ohio State University, Columbus, Ohio (United States); Nyati, Shyam [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Ross, Brian D. [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Rehemtulla, Alnawaz, E-mail: alnawaz@umich.edu [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States)

2013-08-01T23:59:59.000Z

372

4-H Activities  

E-Print Network [OSTI]

4-H activities offer young people many exciting opportunities for personal development. These activities often are a part of their projects. This publication outlines activity options....

Howard, Jeff W.

2005-05-10T23:59:59.000Z

373

Application of neutron activation analysis and high resolution x-ray spectrometry for the determination of trace quantities of elements with short-lived activation products  

E-Print Network [OSTI]

Conversion Interferences and Errors 3, PROCEDURES AND EXPERIMENTAL SETUP Sample Preparation Pneumatic Sample Transfer System Sample Irradiation X-ray Spectrometry Data Reduction 4. DISCUSSION AND RESULTS System Analysis Analysis Problems.... C. Roentgen made the classic observation that a highly penatrative radiation, unknown at that time, was produced when fast electrons impinged on matter. This radiation, which h called x-rays, was being studied in all parts of the world less than...

Marshall, John Richard

1974-01-01T23:59:59.000Z

374

Retrospective radon progeny measurements through measurements of 210 activities on glass objects using stacked LR 115 detectors  

E-Print Network [OSTI]

Retrospective radon progeny measurements through measurements of 210 Po activities on glass objects 2 September 2008 PACS: 29.40 23.60 Keywords: Radon Radon progeny 210 Po Implantation Retrospective for determining 210 Po activity in glass surfaces after deposition of short-lived radon progeny. The sensitivities

Yu, K.N.

375

Search for long-lived particles decaying into electron or photon pairs with the D0 detector  

SciTech Connect (OSTI)

In this Letter we report on a search for long-lived particles that decay into final states with two electrons or photons. Such long-lived particles arise in a variety of theoretical models, like hidden valleys and supersymmetry with gauge-mediated breaking. By precisely reconstructing the direction of the electromagnetic shower we are able to probe much longer lifetimes than previously explored. We see no evidence of the existence of such long-lived particles and interpret this search as a quasi model-independent limit on their production cross section, as well as a limit on a long-lived fourth generation quark.

Abazov, V.M.; /Dubna, JINR; Abbott, B.; /Oklahoma U.; Abolins, M.; /Michigan State U.; Acharya, B.S.; /Tata Inst.; Adams, M.; /Illinois U., Chicago; Adams, T.; /Florida State U.; Aguilo, E.; /Simon Fraser U.; Ahsan, M.; /Kansas State U.; Alexeev, G.D.; /Dubna, JINR; Alkhazov, G.; /St. Petersburg, INP; Alton, A.; /Michigan U. /Northeastern U.

2008-06-01T23:59:59.000Z

376

A Living Mediterranean River: Restoration and Management of the Rio Real in Portugal to Achieve Good Ecological Condition  

E-Print Network [OSTI]

for stormwater treatment wetlands A LIVING MEDITERRANEANharvesting and water treatment wetlands using the theSITES for stormwater treatment wetland areas were identified

Natali, Jennifer; Kondolf, G. Mathias; Landeiro, Clara; Christian-Smith, Juliet; Grantham, Ted

2009-01-01T23:59:59.000Z

377

The living workplace : a conscious work environment for a small publishing company  

E-Print Network [OSTI]

This thesis is about the oneness of working and living, and about the making of workplaces that support and encourage the idea that one's work can be an integral part of one's life. The opening position is that there is a ...

Berg, Richard Carl

1986-01-01T23:59:59.000Z

378

The Pollution Attack in P2P Live Video Streaming: Measurement Results and Defenses  

E-Print Network [OSTI]

The Pollution Attack in P2P Live Video Streaming: Measurement Results and Defenses Prithula Dhungel in the recent years. In this paper, we examine the stream pollution attack, for which the attacker mixes polluted chunks into the P2P distribu- tion, degrading the quality of the rendered media at the receivers

Saxena, Nitesh

379

"Study political science in a city that lives and breathes politics." carleton.ca/polisci  

E-Print Network [OSTI]

"Study political science in a city that lives and breathes politics." carleton.ca/polisci GRADUATE PROGRAMS IN SHAPE YOUR FUTURE BASED ON YOUR RESEARCH INTERESTS Where better to study political science than and PhD program in political science, as well as a collaborative specializa- tion in African Studies and

Dawson, Jeff W.

380

DOES HOSPITAL COMPETITION SAVE LIVES? EVIDENCE FROM THE ENGLISH NHS PATIENT CHOICE REFORMS*  

E-Print Network [OSTI]

DOES HOSPITAL COMPETITION SAVE LIVES? EVIDENCE FROM THE ENGLISH NHS PATIENT CHOICE REFORMS* Zack Cooper, Stephen Gibbons, Simon Jones and Alistair McGuire Recent substantive reforms to the English with fixed prices. This study investigates whether these reforms led to improvements in hospital quality. We

Mateo, Jill M.

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


381

LIVE CARS FOR USE IN CATFISH INDUSTRY Donald C. ,r nland, Rob rt L. ..rill,  

E-Print Network [OSTI]

to deep water, or set adjacent to a well head for holding fish. Haul trucks can be scheduled more easily to loading sit s or shift d to saf areas for holding. When us ·d along Ith a haul Sf ine, pulling accurately because fish in properly staked live cars do not escape and can be harvested before truck arrives

382

Cold Climate Heat Pump Projects at Purdue University & the Living Lab  

E-Print Network [OSTI]

11/10/2011 6 #12;System Design · 19 kW (~65000 Btu/h) at -20 OC (-4 OF) · Install strip electric heat pump optimized for heating » Greatly reduce or eliminate need for auxiliary electric resistance heatingCold Climate Heat Pump Projects at Purdue University & the Living Lab at the new Herrick Labs

Oak Ridge National Laboratory

383

Electro-osmotic flow control for living cell analysis in microfluidic Tomasz Glawdel, Carolyn L. Ren *  

E-Print Network [OSTI]

the problems associated with the use of high electric fields required for operating electro-osmotic pumps in living cell analysis. In particular, electroporation of cell mem- branes, Joule heating, electrolysis pressure-driven flow control systems. Futai et al. (2006) developed a recircu- lating perfusion platform

Le Roy, Robert J.

384

SUBMITTED TO IEEE TRANSACTIONS ON COMPUTERS 1 iAware: Making Live Migration of Virtual  

E-Print Network [OSTI]

on harnessing live migration of VMs to achieve load balancing and power saving among different servers and destination servers during and after such VM migration. To avoid potential violations of service address is bli@cse.ust.hk. · Baochun Li is with the Department of Electrical and Computer Engineering

Li, Baochun

385

Living and working in Germany A guide for international scientists at Max Planck Institutes  

E-Print Network [OSTI]

Living and working in Germany A guide for international scientists at Max Planck Institutes #12 and the hospitality in your town. You will quickly find that Germany is a country of many facets ­ a country Planck Society is Germany's most successful scientific or- ganisation in basic research. Max Planck

386

Study China Programme Live and learn in China for three weeks  

E-Print Network [OSTI]

Study China Programme Live and learn in China for three weeks Study China is a unique learning, arts and business. About Study China Finance is provided by the UK government and managed Universities. Study China is in it's fourth year and has enabled over 1000 students to explore this fascinating

Oakley, Jeremy

387

Updated March 2013 Eat Smart, Live Strong is designed to improve fruit and vegetable consumption and  

E-Print Network [OSTI]

Updated March 2013 Eat Smart, Live Strong is designed to improve fruit and vegetable consumption: · eat at least 3 ½ cups of fruit and vegetable per day (1 ½ cups of fruits and 2 cups of vegetables;Session 1, Reach Your Goals, Step by Step allows participants to review the amount of fruits

388

Alpha-decay half-lives, alpha-capture and alpha-nucleus potential  

E-Print Network [OSTI]

The alpha-decay half-lives and the alpha-capture cross-sections are evaluated in the framework of unified model for alpha-decay and alpha-capture. In the framework of this model the alpha-decay and alpha-capture are considered as penetration of the alpha-particle through the potential barrier formed by nuclear, Coulomb and centrifugal interactions between alpha-particle and nucleus. The spins and the parities of parent and daughter nuclei as well as the quadrupole and hexadecapole deformations of daughter nuclei are taken into account for evaluation of the alpha-decay half-lives. The alpha-decay half-lives for 344 nuclei and the alpha-capture cross-sections of 40Ca, 44Ca, 59Co, 208Pb and 209Bi agree well with the experimental data. The evaluated alpha-decay half-lives within the range 10^{-9}

V. Yu. Denisov; A. A. Khudenko

2009-02-04T23:59:59.000Z

389

A Surface Energy Transfer Nanoruler for Measuring Binding Site Distances on Live Cell Surfaces  

E-Print Network [OSTI]

background significantly reduces the signal-to-background ratio of the plasmonic sensor and greatly hinders SET nanoruler for live cells with long distance, easy construction, fast detection, and low background (LSPR) sensors meet these requirements.8-10 Liu et al.8 constructed a nanoplasmonic molecular ruler

Tan, Weihong

390

Measuring protein mobility by photobleaching GFP-chimeras in living cells  

E-Print Network [OSTI]

1 Measuring protein mobility by photobleaching GFP-chimeras in living cells Erik Snapp, Nihal Altan the fluorophore's fluorescence. The diffusive characteristics of green fluorescent protein (GFP)- chimeras, in which the same region of a cell expressing a GFP-chimera is repetitively photobleached and the loss

Snapp, Erik Lee

391

Demo: Illinois Vehicular Project, Live Data Sampling and Energy-efficient Node Discovery  

E-Print Network [OSTI]

Demo: Illinois Vehicular Project, Live Data Sampling and Energy-efficient Node Discovery Riccardo Crepaldi, Mehedi Bakht, Tarek Abdelzaher, Robin Kravets University of Illinois at Urbana-Champaign {rcrepal2,mbakht2, zaher, rhk}@illinois.edu Embedded sensors in mobile devices such as cars and smart phones

Kravets, Robin

392

Using a Database: Comparative Research with a Standard Sample http:// links are live  

E-Print Network [OSTI]

Chapter 2 Using a Database: Comparative Research with a Standard Sample http:// links are live The first steps in testing hypotheses with a database are to search the codebook for variables relevant to the hypothesis, study how these variables are coded, and decide which variables to retrieve from the database

White, Douglas R.

393

Environmental Factors and Breast Cancer Risk For millions of women whose lives  

E-Print Network [OSTI]

on the ways disease themselves, and are likelyin which environmental exposures increase breast cancer risk will be analyzed for pesticides, heavy metals and other environmental chemicals that may be linked to breast cancerEnvironmental Factors and Breast Cancer Risk For millions of women whose lives have been affected

Bezrukov, Sergey M.

394

Effects of extrinsic and intrinsic factors on breeding success in a long lived seabird  

E-Print Network [OSTI]

. We investigated the relationships between breeding success and environmental conditions (winter NAOEffects of extrinsic and intrinsic factors on breeding success in a long lived seabird Sue Lewis success rate) in the northern fulmar Fulmarus glacialis, using data from a long-term study commenced

Aberdeen, University of

395

Faculty of Architecture and Planning Re-designing the World for 21st century Living  

E-Print Network [OSTI]

A Faculty of Architecture and Planning #12;1 Re-designing the World for 21st century Living A t Dalhousie's Faculty of Architecture and Planning we want to build a better world. And we are starting in our applied art and science we design and build vibrant, sustainable structures and communities -- places

Brownstone, Rob

396

Car Sharing for the Twin Cities ! HOURCAR helps make it possible to live car-  

E-Print Network [OSTI]

Car Sharing for the Twin Cities ! HOURCAR helps make it possible to live car- free or car-lite by filling in the "gaps" ! Car sharing reduces car ownership and saves its members thousands of dollars per year ! Car sharing "right-sizes" and makes transparent the costs of car usage #12;HOURCAR ! Transit

Minnesota, University of

397

The ultimate goal "Deciphering" the DNA nucleotide sequence of a living system*.  

E-Print Network [OSTI]

The ultimate goal "Deciphering" the DNA nucleotide sequence of a living system*. Tools difficult macromolecule to analyse. Now it is possible to determine the nucleotide sequence at a rate of several hundred nucleotide a day. (August 2005: 1011 bases) #12;#12;#12;#12;#12;#12;#12;#12; = 0.283 #12

Morante, Silvia

398

Heart stopping moments with zebrafish: imaging inside the living, beating heart  

E-Print Network [OSTI]

Heart stopping moments with zebrafish: imaging inside the living, beating heart Seamless this application, enabling researchers to carry out precision "healing" studies on the moving heart by targeting of development Jonathan M. Taylor, Glasgow University d "Realtime Optical Gating for 3D Heart Imaging", Journal

Greenaway, Alan

399

Had my water gone bad? Family members have lived on our land  

E-Print Network [OSTI]

to find out. Hated to spend the money, but it gave me peace of mind. " " " We're protecting our family. We for more help. Some labs will do the sampling for you, right at your home. 4. After you collect your waterHad my water gone bad? Family members have lived on our land for generations. Never had a problem

Rhode Island, University of

400

Living Ring-Opening Polymerization of N-Sulfonylaziridines: Synthesis of High Molecular Weight Linear Polyamines  

E-Print Network [OSTI]

Living Ring-Opening Polymerization of N-Sulfonylaziridines: Synthesis of High Molecular Weight@berkeley.edu; fdtoste@berkeley.edu Polymerization of cyclic ethers, particularly epoxides, to generate low limited, despite the utility of such materials.2 Low temperature polymerization of aziridine affords

Toste, Dean

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


401

Living Longer on Less THe neW economic (in)securiTy of seniors  

E-Print Network [OSTI]

Living Longer on Less THe neW economic (in)securiTy of seniors INSTITUTE ON ASSETS & SOCIAL POLICY to measuring economic security applied in this report builds on previous work on middle class economic security for Social Policy and Manage- ment at Brandeis University, is dedicated to the economic well-being and social

Snider, Barry B.

402

Innovation Hubs Kendall Square as Laboratory for High-Density Urban Living  

E-Print Network [OSTI]

% of total energy consumption. It is a global imperative to develop systems that improve the livability of cities while dramatically reducing resource consumption. This workshop will explore new urban systems for high-density cities including systems for mobility, energy, food production, and live

403

@scale: Insights from a Large, Long-Lived Appliance Stephen Dawson-Haggerty  

E-Print Network [OSTI]

Design, Measurement, Performance Keywords Energy, Audit, Building, Power, Wireless, Sensor Network 1@scale: Insights from a Large, Long-Lived Appliance Energy WSN Stephen Dawson-Haggerty , Steven Lanzisera , Jay Taneja , Richard Brown , and David Culler Computer Science Division Environmental Energy

Culler, David E.

404

Multiannual patterns of influenza A transmission in Chinese live bird market systems  

E-Print Network [OSTI]

Multiannual patterns of influenza A transmission in Chinese live bird market systems Kim M. Pepin and emergence in humans is confounded by insufficient empirical data on the ecology and dynamics of AIV in poultry systems. To address this gap, we quantified incidence patterns for 13 hemagglutinin subtypes

Webb, Colleen

405

Incorporation of Short-Lived Be in a Calcium-Aluminum  

E-Print Network [OSTI]

Incorporation of Short-Lived 10 Be in a Calcium-Aluminum­ Rich Inclusion from the Allende Meteorite Kevin D. McKeegan,1 * Marc Chaussidon,2 Franc¸ois Robert3 Enrichments in boron-10/boron-11 in a calcium-aluminum canonical abundance of aluminum-26 may still require seeding of the solar system by radioactive stellar

406

Montana's Water very person who lives in or visits Montana participates in water manage-  

E-Print Network [OSTI]

Managing Montana's Water E very person who lives in or visits Montana participates in water manage management involves people who allocate water sup- plies, issue permits, regulate the resource according to state and federal laws, and enforce laws when violations occur. Without water management, more human

Lawrence, Rick L.

407

hirty years ago,half the developing world lived in extreme poverty--  

E-Print Network [OSTI]

#12;T hirty years ago,half the developing world lived in extreme poverty-- today, a quarter.1 Now the billion mark this year for the first time in history.2 With so many still in poverty and hunger, growth and poverty alleviation remain the overarching priority for develop- ing countries. Climate change only makes

Kammen, Daniel M.

408

Method of freezing living cells and tissues with improved subsequent survival  

DOE Patents [OSTI]

This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.

Senkan, Selim M. (Oak Ridge, TN); Hirsch, Gerald P. (Oak Ridge, TN)

1980-01-01T23:59:59.000Z

409

Predicting the fate of a living fossil: how will global warming affect sex determination  

E-Print Network [OSTI]

Predicting the fate of a living fossil: how will global warming affect sex determination, an unlikely response to global warming, as many oviparous species are nesting earlier as the climate warms. Keywords: climate change; global warming; temperature-dependent sex determination; reptile; Sphenodon 1

Sheridan, Jennifer

410

A C. elegans-based foam for rapid on-site detection of residual live virus.  

SciTech Connect (OSTI)

In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

2012-02-01T23:59:59.000Z

411

Challenges in recording and stimulation of living neural network based on Original Micro-Electrode Array  

E-Print Network [OSTI]

Challenges in recording and stimulation of living neural network based on Original Micro in bioelectronics can lead to neuroscience applications to explore the properties of neural networks. Micro properties of neural networks. Recent advances in micro and nano technology have opened the way to probe

Paris-Sud XI, Université de

412

The University of New Hampshire in 2020: Breaking Silos, Transforming Lives, Reimagining the University  

E-Print Network [OSTI]

their historic mission and respond energetically and effectively to these new challenges? The University of New1 The University of New Hampshire in 2020: Breaking Silos, Transforming Lives, Reimagining of our state, our nation and our world. For 143 years we have served the people and communities of New

413

Search for long-lived gravitational-wave transients coincident with long gamma-ray bursts  

E-Print Network [OSTI]

Long gamma-ray bursts (GRBs) have been linked to extreme core-collapse supernovae from massive stars. Gravitational waves (GW) offer a probe of the physics behind long GRBs. We investigate models of long-lived (?10–1000??s) ...

Aggarwal, Nancy

414

Polyacrylamide nanosensor embedded with phosphate sensitive protein for detection of metabolic process in living cells  

E-Print Network [OSTI]

Energy Transfer (FRET) efficiency.2 Fluorescent nanosensor particles, in which the fluorescent probes of phosphate is required to study phosphate homeostatis in living cell. Very few sensors reported display high, and at the same time, (b) fluorescent protein from any potential interferences such as deactivation by protease

415

We don't just teach sustainability--we live it!  

E-Print Network [OSTI]

#12;We don't just teach sustainability-- we live it! Green comes to life every day at the University of Oregon's Center for Sustainable Business Practices. Housed at the Lundquist College of Business, and financial responsibilities. For us, the market demand for transparency, sustainability, and business

416

Origin and Phylogeny of Microbes Living in Permanent Antarctic Lake Ice  

E-Print Network [OSTI]

Origin and Phylogeny of Microbes Living in Permanent Antarctic Lake Ice D. A. Gordon,1, * J. Priscu of bacteria and cyanobacteria colonizing sediment particles in the per- manent ice cover of an Antarctic lake collected from a depth of 2.5 m in the 4-m-thick ice cover of Lake Bonney, McMurdo Dry Valleys, Antarctica

Priscu, John C.

417

GREEN LIVING Replace incandencent and halogen light bulbs with LED and CLFs  

E-Print Network [OSTI]

GREEN LIVING GUIDE ENERGY TRAVEL FOOD sustain yosef WATER Replace incandencent and halogen light for your laundry RESIST THAT SWITCH! Use natural light during the day, and no lights when you are gone USE MORE THAN YOU CAN EAT, reduce your waste stream DRINK FAIR TRADE COFFEE - Check out Conrad

Thaxton, Christopher S.

418

Living on the Edge: Agriculture in Periurban Mexico City Andy Wanning  

E-Print Network [OSTI]

Living on the Edge: Agriculture in Periurban Mexico City Andy Wanning Spring 2014 Capstone M.S. Sustainability Management Isla Urbana installs rainwater harvesting systems in and around Mexico City so that people aren't forced to get water via... Sistema Biobolsa installs biodigesters throughout Mexico so that

419

Lecture Notes in Computer Science Towards a Living Software Development Process  

E-Print Network [OSTI]

Lecture Notes in Computer Science Towards a Living Software Development Process based on Process Process for a certain enterprise and/or a certain project will usually integrate elements from a variety of existing process models, comprising generic standards as well as specific development methods. Besides that

420

This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research  

E-Print Network [OSTI]

and endophyte were collected. The extract was inactive to the human parasites Trypanosoma cruzi, Leishmania reserved. Introduction Endophytes are microorganisms including bacteria or fungi that live within,2 Endophytic microbes have been recognized as important sources of structurally novel and biologically active

Coley, Phyllis

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


421

The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells  

SciTech Connect (OSTI)

Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ? Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ? Tri-molecular complexes containing two scFvs and one molecule GK were formed. ? GK was detected using fluorescence lifetime-based FRET imaging. ? GK is monomeric and homogeneously distributed in several cancer cell lines. ? This technique may have many applications in live-cell imaging of endogenous proteins.

Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

2013-04-01T23:59:59.000Z

422

Coming out and living openly are not something you do once, or even for one year. It is a journey that we make every single day of our lives. Every coming  

E-Print Network [OSTI]

making a coming out plan Coming out and living openly are not something you do once, or even and are not ready to identify as being lesbian, gay, bisexual or transgender. That's OK. Maybe you just want to tell and unfolds at your own pace. · Living openly is something that becomes easier with time. Even after you

de Lijser, Peter

423

Lively Infrastructure  

E-Print Network [OSTI]

tanks assembled for the houses, the poles, wires and tubes put into place to pirate water and electricity, the spaces cleared for play areas, churches, roads and toilet blocs, and the vehicles, mobile phones and televisions facilitating contact...

Amin, Ash

2014-10-06T23:59:59.000Z

424

Live Status  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: VegetationEquipment Surfaces and Interfaces Sample6, 2011LiisaInnovation Portal SolarLittleageFor

425

Live Status  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May Jun Jul(Summary)morphinanInformation Desert Southwest Region serviceMission StatementCenterTri-Party Agreement > Queue Look

426

Phase separation and coarsening in active matter  

E-Print Network [OSTI]

Active systems, or active matter, are self-driven systems which live, or function, far from equilibrium - a paradigmatic example which we focus on here is provided by a suspension of self-motile particles. Active systems are far from equilibrium because their microscopic constituents constantly consume energy from the environment in order to do work, for instance to propel themselves. The nonequilibrium nature of active matter leads to a variety of non-trivial intriguing phenomena. An important one which has recently been the subject of intense interest among biological and soft matter physicists is that of the so-called "motility-induced phase separation", whereby self-propelled particles accumulate into clusters in the absence of any explicit attractive interactions between them. Here we review the physics of motility-induced phase separation, and discuss this phenomenon within the framework of the classic physics of phase separation and coarsening. We also discuss cases where the coarsening may be arrested, either in theories for bacterial colonies or in experiments. Most of this work will focus on the case of run-and-tumble and active Brownian particles in the absence of solvent-mediated hydrodynamic interactions - we will briefly discuss at the end their role, which is not currently fully understood in this context.

Giuseppe Gonnella; Davide Marenduzzo; Antonio Suma; Adriano Tiribocchi

2015-02-08T23:59:59.000Z

427

OPTICAL NANOSENSOR PARTICLES FOR DETECTION OF pH IN LIVING CELLS A. M. Scharff-Poulsena  

E-Print Network [OSTI]

H sensitive dye and the reference dye permit intracellular pH measurements by fluorescence ratio imagingOPTICAL NANOSENSOR PARTICLES FOR DETECTION OF pH IN LIVING CELLS A. M. Scharff-Poulsena , H. Suna is limited by the lack of tools for measuring of metabolite levels in living cells with high spatial

428

LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Photography Protocol.docx revised 10/1/13 by JW Page 1 of 2  

E-Print Network [OSTI]

LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Photography Protocol.docx revised 10/1/13 by JW Page 1 of 2 Live Fish Photography Protocol Set up: Stand the white board used for preserved to the white board. Position camera so that fish area fills view. Make sure auto focus is on. Make camera

Langerhans, Brian

429

Global analysis of Fo rster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting  

E-Print Network [OSTI]

Global analysis of Fo¨ rster resonance energy transfer in live cells measured by fluorescence of Fo¨ rster resonance energy transfer (FRET) in live cells using the rise time of acceptor fluorescence those molecules that are involved in the energy-transfer process are monitored. This contrasts

van Stokkum, Ivo

430

Book review: Elizabeth E. Watson, 2009, Living Terraces in Ethiopia Konso Landscape, Culture & Development, Woodbridge and New York: James Currey  

E-Print Network [OSTI]

1 Book review: Elizabeth E. Watson, 2009, Living Terraces in Ethiopia ­ Konso Landscape, Culture'Ethiopie. Quotation: Demeulenaere, Elise. 2010. "Book review of Watson E. E., 2009, 'Living Terraces in Ethiopia territory in southern Ethiopia entirely shaped by agricultural terraces. For the author, most existing

Paris-Sud XI, Université de

431

Tuned, driven, and active soft matter  

E-Print Network [OSTI]

One characteristic feature of soft matter systems is their strong response to external stimuli. As a consequence they are comparatively easily driven out of their ground state and out of equilibrium, which leads to many of their fascinating properties. Here, we review illustrative examples. This review is structured by an increasing distance from the equilibrium ground state. On each level, examples of increasing degree of complexity are considered. In detail, we first consider systems that are quasi-statically tuned or switched to a new state by applying external fields. These are common liquid crystals, liquid crystalline elastomers, or ferrogels and magnetic elastomers. Next, we concentrate on systems steadily driven from outside e.g. by an imposed flow field. In our case, we review the reaction of nematic liquid crystals, of bulk-filling periodically modulated structures such as block copolymers, and of localized vesicular objects to an imposed shear flow. Finally, we focus on systems that are "active" and "self-driven". Here our range spans from idealized self-propelled point particles, via sterically interacting particles like granular hoppers, via microswimmers such as self-phoretically driven artificial Janus particles or biological microorganisms, via deformable self-propelled particles like droplets, up to the collective behavior of insects, fish, and birds. As we emphasize, similarities emerge in the features and behavior of systems that at first glance may not necessarily appear related. We thus hope that our overview will further stimulate the search for basic unifying principles underlying the physics of these soft materials out of their equilibrium ground state.

Andreas M. Menzel

2015-01-28T23:59:59.000Z

432

Activated Boron Nitride Derived from Activated Carbon  

E-Print Network [OSTI]

combination of chemical, thermal, and electrical properties. The utility of activated carbon suggests is characterized by scanning electron microscopy, high-resolution transmission electron microscopy, electron energy loss spectroscopy, and surface area analysis. The activated BN microstructure is similar

Zettl, Alex

433

Secondary Energy Infobook Activities (19 Activities)'  

Broader source: Energy.gov (indexed) [DOE]

Infobook Activities (19 Activities) Grades: 9-12 Topics: Energy Basics Owner: NEED This educational material is brought to you by the U.S. Department of Energy's Office of Energy...

434

Cell-Permeable Near-Infrared Fluorogenic Substrates for Imaging -Lactamase Activity  

E-Print Network [OSTI]

Cell-Permeable Near-Infrared Fluorogenic Substrates for Imaging -Lactamase Activity Bengang Xing,11 Several fluorogenic substrates for Bla have been reported,4,12 but none work for infrared or near-infrared fluorescence imaging. Infrared/near-infrared light is preferred in molecular imaging studies of living subjects

Xing, Bengang

435

Total and spontaneous fission half-lives for americium and curium nuclides  

SciTech Connect (OSTI)

The long-lived nuclides of the americium curium elements are of interest for their use in certain safeguard applications and for nuclear reactor burnup studies in waste management. Recommended values are presented for /sup 241,242m,243/Am, and for /sup 242,243,244,245,246,247,248,250/Cm. These values result from a consistent evaluation of all these half-lives. These preliminary estimates were presented earlier. The uncertainties are provided at the 95% confidence limit for each of the recommended values. It will be noted that many of the recommended errors considerably exceed errors quoted by individual authors in their publication, by up to an order of magnitude, e.g., the total half-life of /sup 242,246,248/Cm and the spontaneous fission half-life of /sup 244/Cm. 65 refs., 18 tabs.

Holden, N.E.

1985-01-01T23:59:59.000Z

436

FRACTURE MECHANICS APPROACH TO ESTIMATE FATIGUE LIVES OF WELDED LAP-SHEAR SPECIMENS  

SciTech Connect (OSTI)

A full range of stress intensity factor solutions for a kinked crack is developed as a function of weld width and the sheet thickness. When used with the associated main crack solutions (global stress intensity factors) in terms of the applied load and specimen geometry, the fatigue lives can be estimated for the laser-welded lap-shear specimens. The estimations are in good agreement with the experimental data. A classical solution for an infinitesimal kink is also employed in the approach. However, the life predictions tend to overestimate the actual fatigue lives. The traditional life estimations with the structural stress along with the experimental stress-fatigue life data (S-N curve) are also provided. In this case, the estimations only agree with the experimental data under higher load conditions.

Lam, P.; Michigan, J.

2014-04-25T23:59:59.000Z

437

Structure and Growth in the Living Tissue and in Carbon Nanotubes  

E-Print Network [OSTI]

The topological organisation of cells in a model of living tissue (the crypt of intestinal epithelium) is identical to the topological organisation of atoms in carbon nanotubes. The existing models of growth of these two structures contain identical elements. It is proposed that the growth of carbon nanotubes can depend also on other mechanisms postulated in the crypt model where the growth in the bottom is transmitted to the cylinder, and, in this case, the growth of nanotubes should involve the same peculiar lattice transformations as the ones found in the crypt models. The crypt models also suggest a possible initiation of growth in the nanotubes by the loss of carbon atoms. We consider the crystalline structures that can be formed from graphene and the structures of the living tissues as one distinct class of structures.

Michael Pyshnov; Sergei Fedorov

2011-06-28T23:59:59.000Z

438

Density waves in the shearing sheet IV. Interaction with a live dark halo  

E-Print Network [OSTI]

It is shown that if the self-gravitating shearing sheet, a model of a patch of a galactic disk, is embedded in a live dark halo, this has a strong effect on the dynamics of density waves in the sheet. I describe how the density waves and the halo interact via halo particles either on orbits in resonance with the wave or on non-resonant orbits. Contrary to expectation the presence of the halo leads to a very considerable enhancement of the amplitudes of the density waves in the shearing sheet. This effect appears to be the equivalent of the recently reported enhanced growth of bars in numerically simulated stellar disks embedded in live dark halos. Finally I discuss the transfer of linear momentum from a density wave in the sheet to the halo and show that it is mediated only by halo particles on resonant orbits.

B. Fuchs

2004-03-01T23:59:59.000Z

439

Nuclear Half-Lives for Alpha Radioactivity of Elements with 100 $\\leq$ Z $\\leq$ 130  

E-Print Network [OSTI]

Theoretical estimates for the half lives of about 1700 isotopes of heavy elements with Z from 100 to 130 are tabulated using theoretical Q-values. The quantum mechanical tunneling probabilities are calculated within a WKB framework using microscopic nuclear potentials. The microscopic nucleus-nucleus potentials are obtained by folding the densities of interacting nuclei with a density dependent M3Y (DDM3Y) effective nucleon-nucleon interaction. The alpha-decay half lives calculated in this formalism using the experimental Q-values were found to be in good agreement over a wide range of experimental data spanning about twenty orders of magnitude. The theoretical Q-values used for the present calculations are extracted from three different mass estimates viz. Myers-Swiatecki [MS], Muntian-Hofmann-Patyk-Sobiczewski [M] and Koura-Tachibana-Uno-Yamada [KUTY].

P. Roy Chowdhury; C. Samanta; D. N. Basu

2008-02-29T23:59:59.000Z

440

Shell model half-lives for r-process N=82 nuclei  

E-Print Network [OSTI]

We have performed shell-model calculations of the half-lives and neutron-branching probabilities of the r-process waiting point nuclei at the magic neutron number N=82. These new calculations use a larger model space than previous shell model studies and an improved residual interaction which is adjusted to recent spectroscopic data around A=130. Our shell-model results give a good account of all experimentally known half-lives and $Q_\\beta$-values for the N=82 r-process waiting point nuclei. Our half-life predictions for the N=82 nuclei with Z=42--46 agree well with recent estimates based in the energy-density functional method.

J. J. Cuenca-Garcia; G. Martinez-Pinedo; K. Langanke; F. Nowacki; I. N. Borzov

2008-11-07T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


441

Total and spontaneous fission half-lives of the americium and curium nuclides  

SciTech Connect (OSTI)

The total half-life and the half-life for spontaneous fission are evaluated for the various long-lived nuclides of interest. Recommended values are presented for /sup 241/Am, /sup 242m/Am, /sup 243/Am, /sup 242/Cm, /sup 243/Cm, /sup 244/Cm, /sup 245/Cm, /sup 246/Cm, /sup 247/Cm, /sup 248/Cm, and /sup 250/Cm. The uncertainties are provided at the 95% confidence limit for each of the recommended values.

Holden, N.E.

1984-01-01T23:59:59.000Z

442

Imaging single cells in a beam of live cyanobacteria with an X-ray laser  

DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.

Schot, Gijs, vander

443

Predicting carcass cutability of Rambouillet rams using live or carcass measures  

E-Print Network [OSTI]

analyses. Live measurement. s were adjusted to slaughter weight. Carcass measurements were adjusted in a like manner to carcass weight. Simple correlation coefficients were greatly reduced and most were found to be insignificant after adjustment... as measured by the weight placed on the hind legs were the four variables found most useful in a prediction equation estimating weight of primal cuts 2= (R = . 82). Weight of total consumer cuts was predicted with less 2= accuracy (R = , 64) by slaughter...

Snowder, Gary Douglas

1980-01-01T23:59:59.000Z

444

Big-bang nucleosynthesis with a long-lived CHAMP including He4 spallation process  

E-Print Network [OSTI]

We propose helium-4 spallation processes induced by long-lived stau in supersymmetric standard models, and investigate an impact of the processes on light elements abundances. We show that, as long as the phase space of helium-4 spallation processes is open, they are more important than stau-catalyzed fusion and hence constrain the stau property. This talk is based on works (Jittoh et al., 2011).

Toshifumi Jittoh; Kazunori Kohri; Masafumi Koike; Joe Sato; Kenichi Sugai; Masato Yamanaka; Koichi Yazaki

2012-09-10T23:59:59.000Z

445

Assembly and actuation of nanomaterials using active biomolecules.  

SciTech Connect (OSTI)

The formation and functions of living materials and organisms are fundamentally different from those of synthetic materials and devices. Synthetic materials tend to have static structures, and are not capable of adapting to the functional needs of changing environments. In contrast, living systems utilize energy to create, heal, reconfigure, and dismantle materials in a dynamic, non-equilibrium fashion. The overall goal of the project was to organize and reconfigure functional assemblies of nanoparticles using strategies that mimic those found in living systems. Active assembly of nanostructures was studied using active biomolecules to drive the organization and assembly of nanocomposite materials. In this system, kinesin motor proteins and microtubules were used to direct the transport and interactions of nanoparticles at synthetic interfaces. In addition, the kinesin/microtubule transport system was used to actively assemble nanocomposite materials capable of storing significant elastic energy. Novel biophysical measurement tools were also developed for measuring the collective force generated by kinesin motor proteins, which will provide insight on the mechanical constraints of active assembly processes. Responsive reconfiguration of nanostructures was studied in terms of using active biomolecules to mediate the optical properties of quantum dot (QD) arrays through modulation of inter-particle spacing and associated energy transfer interaction. Design rules for kinesin-based transport of a wide range of nanoscale cargo (e.g., nanocrystal quantum dots, micron-sized polymer spheres) were developed. Three-dimensional microtubule organizing centers were assembled in which the polar orientation of the microtubules was controlled by a multi-staged assembly process. Overall, a number of enabling technologies were developed over the course of this project, and will drive the exploitation of energy-driven processes to regulate the assembly, disassembly, and dynamic reorganization of nanomaterials.

Spoerke, Erik David; Thayer, Gayle Echo; de Boer, Maarten Pieter; Bunker, Bruce Conrad; Liu, Jun; Corwin, Alex David; Gaudioso, Jennifer Marie; Sasaki, Darryl Yoshio; Boal, Andrew Kiskadden; Bachand, George David; Trent, Amanda M.; Bachand, Marlene; Rivera, Susan B.; Koch, Steven John

2005-11-01T23:59:59.000Z

446

Americium and curium total half-lives and for the spontaneous fission branch  

SciTech Connect (OSTI)

The long-lived nuclides of the americium and curium elements are of interest for their use in certain safeguard applications and for nuclear reactor burnup studies in waste management. Recommended values are presented for /sup 241,242m,243/Am, and for /sup 242,243,244,245,246,247,248,250/Cm. These values result from a consistent evaluation of all these half-lives. These preliminary estimates were presented earlier. The uncertainties are provided at the 95% confidence limit for each of the recommended values. It will be noted that many of the recommended errors considerably exceed errors quoted by individual authors in their publication, by up to an order of magnitude, e.g. the total half-life of /sup 242,246,248/Cm and the spontaneous fission half-life of /sup 244/Cm. These preliminary estimates for the half-lives were given previously. Efforts continue to reevaluate the various experiments to better gauge the systematic errors involved and reassess the total error.

Holden, N.E.

1985-01-01T23:59:59.000Z

447

Predictions of Alpha Decay Half lives of Heavy and Superheavy Elements  

E-Print Network [OSTI]

Theoretical estimates for the lifetimes of several isotopes of heavy elements with Z=102-120 are presented by calculating the quantum mechanical tunneling probability in a WKB framework and using microscopic nucleus-nucleus potential obtained by folding the densities of interacting nuclei with the DDM3Y effective nuclear interaction. The $\\alpha$-decay half lives calculated in this formalism using the experimental $Q$-values are in good agreement over a wide range of experimental data. Half lives are also calculated using $Q$-values extracted from two mass formulae. The Viola-Seaborg-Sobiczewski (VSS) estimates of $\\alpha$-decay half lives with the same $Q$-values are presented for comparison. The half life calculations are found to be quite sensitive to the choice of $Q$-values. Comparison with the experimental data delineates the inadequacies of older mass predictions in the domain of heavy and superheavy elements as compared to the newer one by Muntian-Hofmann-Patyk-Sobiczewski, and highlights necessity of a more accurate mass formula which can predict $Q$-values with even higher precision.

C. Samanta; P. Roy Chowdhury; D. N. Basu

2007-05-28T23:59:59.000Z

448

Search for a Neutral Long-Lived Particle Decaying to B-Jets  

SciTech Connect (OSTI)

The existence of the Higgs boson is required by the Standard Model of particle physics, yet it has not been observed. The precise nature of the Higgs boson is unknown and the mechanism by which it interacts with known Standard Model particles is also not known. Long-lived, electrically neutral hadrons have recently been proposed in hidden-valley models and could constitute a pathway through which the Higgs boson communicates with the Standard Model. Such a scenario may provide a novel path to Higgs discovery at the Tevatron. This thesis describes a search for a neutral, long-lived particle produced in decays of Higgs bosons in p{bar p} collisions at a center-of-mass energy of {radical}s = 1.96 TeV, which decays to b-jets and lives long enough to travel at least 1.6 cm before decaying. This analysis uses 3.65 fb{sup -1} of data recorded with the Run II D0 detector at the Fermilab Tevatron collider from April 2002 to August of 2008. We perform a search for eight possible hidden-valley scenarios resulting from a Higgs decay. No significant excess over background is observed and cross-section limits are placed at 95% CL.

Johnson, Chad; /Columbia U.

2009-04-01T23:59:59.000Z

449

Activity Based Costing  

Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

Activity Based Costing (ABC) is method for developing cost estimates in which the project is subdivided into discrete, quantifiable activities or a work unit. This chapter outlines the Activity Based Costing method and discusses applicable uses of ABC.

1997-03-28T23:59:59.000Z

450

Michael Flachsel Active Directory  

E-Print Network [OSTI]

1 Michael Flachsel Active Directory Allgemeiner Aufbau & Struktur an der TUB 6. Juni 2007 Inhalt Directory" #12;2 Inhalt Motivation Grundlagen Motivation Grundlagen Warum Active Directory Grundlagen gemeldeten Typen (c) 2007 Michael Flachsel ,,Active Directory" Inhalt Motivation Grundlagen Motivation

Berlin,Technische Universität

451

Light Activated Self-Propelled Colloids  

E-Print Network [OSTI]

Light-activated self-propelled colloids are synthesized and their active motion is studied using optical microscopy. We propose a versatile route using different photoactive materials, and demonstrate a multiwavelength activation and propulsion. Thanks to the photoelectrochemical properties of two semiconductor materials (\\alpha Fe2 O3 and TiO2 ), a light with an energy higher than the bandgap triggers the reaction of decomposition of hydrogen peroxide and produces a chemical cloud around the particle. It induces a phoretic attraction with neighbouring colloids as well as an osmotic self- propulsion of the particle on the substrate. We use these mechanisms to form colloidal cargos as well as self-propelled particles where the light-activated component is embedded into a dielectric sphere. The particles are self-propelled along a direction otherwise randomized by thermal fluctuations, and exhibit a persistent random walk. For sufficient surface density, the particles spontaneously form "living crystals" which are mobile, break apart and reform. Steering the particle with an external magnetic field, we show that the formation of the dense phase results from the collisions heads-on of the particles. This effect is intrinsically non-equilibrium and a novel principle of organization for systems without detailed balance. Engineering families of particles self-propelled by different wavelength demonstrate a good understanding of both the physics and the chemistry behind the system and points to a general route for designing new families of self-propelled particles.

J. Palacci; S. Sacanna; S. -H. Kim; G. -R. Yi; D. J. Pine; P. M. Chaikin

2014-10-27T23:59:59.000Z

452

Active stewardship: sustainable future  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Active stewardship: sustainable future Active stewardship: sustainable future Energy sustainability is a daunting task: How do we develop top-notch innovations with some of the...

453

University Housing Departmental Performance Blueprint 2010-2011 Academic Year University Housing creates a sustainable living and learning community that promotes the academic success  

E-Print Network [OSTI]

Housing creates a sustainable living and learning community that promotes the academic success environments. #12;2 Contextual Statement Mission Statement: University Housing creates a sustainable living and personal development of students. Vision Sustainable Excellence: Great Today -- Greater Tomorrow Unit

Almor, Amit

454

Roberta E. Rikli, C. Jessie Jones. (1997). Assessing Physical Performance in Independent Older Adults: Issues and Guidelines. Journal of Aging and Physical Activity, 5 (3).  

E-Print Network [OSTI]

Adults: Issues and Guidelines. Journal of Aging and Physical Activity, 5 (3). With the projected growth measure physical performance on a continuum across the wide range of functioning in the independent parameters associated with common activities of daily living. Additional tools are needed for measuring

de Lijser, Peter

455

On campus, c. 1997 Armed with two degrees in Music History and having lived in Russia for  

E-Print Network [OSTI]

On campus, c. 1997 Armed with two degrees in Music History and having lived in Russia for over. He chose Princeton because, at the time, there were no leading experts on Russia who might try

456

Energy Conservation and Comfort of Heat Pump Desiccant Air Conditioning System in Actual Living Space in Summer  

E-Print Network [OSTI]

Energy Conservation and Comfort of Heat Pump Desiccant Air Conditioning System in Actual Living and total heat exchanger in terms of both energy conservation and thermal comfort in summer. 1. COP

Miyashita, Yasushi

457

Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging  

E-Print Network [OSTI]

Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. ...

White, Katharine Alice

2012-01-01T23:59:59.000Z

458

Moving past: probing the agency and affect of recordkeeping in individual and community lives in post-conflict Croatia  

E-Print Network [OSTI]

settlers in post- conflict Croatia, Intl J Hum Rights 17:Northern Ireland and Croatia, Fletcher F. World Aff 16:29-50Lives in Post-conflict Croatia --Manuscript Draft--

Gilliland, AJ

2014-01-01T23:59:59.000Z

459

Development of High-throughput and Robust Microfluidic Live Cell Assay Platforms for Combination Drug and Toxin Screening  

E-Print Network [OSTI]

the labor-intensive and time-consuming limitations, they are too costly for general users. Microfluidic live cell screening platforms can allow precise control of cell culture microenvironments by applying accurate doses of biomolecular mixtures...

Wang, Han

2012-02-14T23:59:59.000Z

460

Growing Up in Scotland: Year 2 - Results from the second year of a study following the lives of Scotland's children   

E-Print Network [OSTI]

The Growing Up in Scotland study (GUS) is an important longitudinal research project aimed at tracking the lives of a cohort of Scottish children from the early years, through childhood and beyond. Its principal aim is ...

Bradshaw, Paul; Cunningham-Burley, Sarah; Dobie, Fiona; MacGregor, Andy; Marryat, Louise; Ormston, Rachel; Wasoff, Fran

2008-02-18T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


461

as Ag Biotech Lived Up to Its Promise? (and what should the scientific community do about it?)  

E-Print Network [OSTI]

H as Ag Biotech Lived Up to Its Promise? (and what should the scientific community do about it?) E that conventional plant breeding could do - and better. He also had a section entitled Standing Up

Clark, E. Ann

462

Study on lipid droplet dynamics in live cells and fluidity changes in model bacterial membranes using optical microscopy techniques   

E-Print Network [OSTI]

In this thesis optical microscopy techniques are used to consider aspects of viral and bacterial infections. In part 1, the physical effects of cytomegalovirus on lipid droplet dynamics in live cells are studied; in part ...

Wong, Christine Shiang Yee

2014-06-28T23:59:59.000Z

463

Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells  

E-Print Network [OSTI]

We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell’s buoyant ...

Cermak, Nathan

464

Collective dynamics of active cytoskeletal networks  

E-Print Network [OSTI]

Self organization mechanisms are essential for the cytoskeleton to adapt to the requirements of living cells. They rely on the intricate interplay of cytoskeletal filaments, crosslinking proteins and molecular motors. Here we present an in vitro minimal model system consisting of actin filaments, fascin and myosin-II filaments exhibiting pulsative collective long range dynamics. The reorganizations in the highly dynamic steady state of the active gel are characterized by alternating periods of runs and stalls resulting in a superdiffusive dynamics of the network's constituents. They are dominated by the complex competition of crosslinking molecules and motor filaments in the network: Collective dynamics are only observed if the relative strength of the binding of myosin-II filaments to the actin network allows exerting high enough forces to unbind actin/fascin crosslinks. The feedback between structure formation and dynamics can be resolved by combining these experiments with phenomenological simulations based on simple interaction rules.

Simone Köhler; Volker Schaller; Andreas R. Bausch

2011-05-23T23:59:59.000Z

465

Developing new optical imaging techniques for single particle and molecule tracking in live cells  

SciTech Connect (OSTI)

Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian cells. New rotational information was obtained: (1) during endocytosis, cargoes lost their rotation freedom at the late stage of internalization; (2) cargoes performed train-like motion when they were transported along the microtubule network by motor proteins inside live cells; (3) During the pause stage of fast axonal transport, cargoes were still bound to the microtubule tracks by motor proteins. Total internal reflection fluorescence microscopy (TIRFM) is another non-invasive and far-field optical imaging technique. Because of its near-field illumination mechanism, TIRFM has better axial resolution than epi-fluorescence microscopy and confocal microscopy. In this work, an auto-calibrated, prism type, angle-scanning TIRFM instrument was built. The incident angle can range from subcritical angles to nearly 90{sup o}, with an angle interval less than 0.2{sup o}. The angle precision of the new instrument was demonstrated through the finding of the surface plasmon resonance (SPR) angle of metal film coated glass slide. The new instrument improved significantly the precision in determining the axial position. As a result, the best obtained axial resolution was {approx} 8 nm, which is better than current existing instruments similar in function. The instrument was further modified to function as a pseudo TIRF microscope. The illumination depth can be controlled by changing the incident angle of the excitation laser beam or adjusting the horizontal position of the illumination laser spot on the prism top surface. With the new technique, i.e., variable-illumination-depth pseudo TIRF microscopy, the whole cell body from bottom to top was scanned.

Sun, Wei

2010-12-15T23:59:59.000Z

466

Search for long-lived isomeric states in neutron-deficient thorium isotopes  

SciTech Connect (OSTI)

The discovery of naturally occurring long-lived isomeric states (t{sub 1/2}>10{sup 8} yr) in the neutron-deficient isotopes {sup 211,213,217,218}Th[A. Marinov et al., Phys. Rev. C 76, 021303(R) (2007)] was reexamined using accelerator mass spectrometry (AMS). Because AMS does not suffer from molecular isobaric background in the detection system, it is an extremely sensitive technique. Despite our up to two orders of magnitude higher sensitivity we cannot confirm the discoveries of neutron-deficient thorium isotopes and provide upper limits for their abundances.

Lachner, J.; Dillmann, I.; Faestermann, T.; Korschinek, G.; Poutivtsev, M.; Rugel, G. [Physik Department E12 and E15, Technische Universitaet Muenchen, D-85748 Garching (Germany)

2008-12-15T23:59:59.000Z

467

Whole-body effective half-lives for radiolabeled antibodies and related issues  

SciTech Connect (OSTI)

Radiolabeled antibodies (RABs) are being developed and used in medical imaging and therapy in rapidly increasing numbers. Data on the whole body half effective half-lives were calculated from external dose rates obtained from attending physicians and radiation safety officers at participating institutions. Calculations were made using exponential regression analysis of data from patients receiving single and multiple administrations. Theses data were analyzed on the basis of age, sex, isotope label, radiation energy, antibody type, disease treated, administration method, and number of administrations.

Kaurin, D.G.L.; Carsten, A.L.; Baum, J.W.; Barber, D.E.

1996-08-01T23:59:59.000Z

468

Animals In Synchrotrons: Overcoming Challenges For High-Resolution, Live, Small-Animal Imaging  

SciTech Connect (OSTI)

Physiological studies in small animals can be complicated, but the complexity is increased dramatically when performing live-animal synchrotron X-ray imaging studies. Our group has extensive experience in high-resolution live-animal imaging at the Japanese SPring-8 synchrotron, primarily examining airways in two-dimensions. These experiments normally image an area of 1.8 mmx1.2 mm at a pixel resolution of 0.45 {mu}m and are performed with live, intact, anaesthetized mice.There are unique challenges in this experimental setting. Importantly, experiments must be performed in an isolated imaging hutch not specifically designed for small-animal imaging. This requires equipment adapted to remotely monitor animals, maintain their anesthesia, and deliver test substances while collecting images. The horizontal synchrotron X-ray beam has a fixed location and orientation that limits experimental flexibility. The extremely high resolution makes locating anatomical regions-of-interest slow and can result in a high radiation dose, and at this level of magnification small animal movements produce motion-artifacts that can render acquired images unusable. Here we describe our experimental techniques and how we have overcome several challenges involved in performing live mouse synchrotron imaging.Experiments have tested different mouse strains, with hairless strains minimizing overlying skin and hair artifacts. Different anesthetics have also be trialed due to the limited choices available at SPring-8. Tracheal-intubation methods have been refined and controlled-ventilation is now possible using a specialized small-animal ventilator. With appropriate animal restraint and respiratory-gating, motion-artifacts have been minimized. The animal orientation (supine vs. head-high) also appears to affect animal physiology, and can alter image quality. Our techniques and image quality at SPring-8 have dramatically improved and in the near future we plan to translate this experience to the Imaging and Medical Beamline at the Australian Synchrotron.Overcoming these challenges has permitted increasingly sophisticated imaging of animals with synchrotron X-rays, and we expect a bright future for these techniques.

Donnelley, Martin [Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, South Australia (Australia); Parsons, David [Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, South Australia (Australia); Women's and Children's Health Research Institute, Adelaide, South Australia (Australia); Department of Paediatrics and Reproductive Health, University of Adelaide, South Australia (Australia); Department of Paediatircs Centre for Stem Cell Research, University of Adelaide, South Australia (Australia); Morgan, Kaye [School of Physics, Monash University, Melbourne, Victoria (Australia); Siu, Karen [School of Physics, Monash University, Melbourne, Victoria (Australia); Monash Centre for Synchrotron Science, Monash University, Melbourne, Victoria (Australia)

2010-07-23T23:59:59.000Z

469

UTSA's SmartLiving Campus: A Real Time Approach to America's Future  

E-Print Network [OSTI]

UTSA SmartLiving CampusA Real-Time Approach to America’s Future ESL-KT-13-12-22 CATEE 2013: Clean Air Through Energy Efficiency Conference, San Antonio, Texas Dec. 16-18 Energy efficiency and conservation are two cost-effective, yet often...-12-22 CATEE 2013: Clean Air Through Energy Efficiency Conference, San Antonio, Texas Dec. 16-18 SOURCE: 2003 CBECS Nationwide: 98% of Bldgs <100K SF Commercial Buildings ESL-KT-13-12-22 CATEE 2013: Clean Air Through Energy Efficiency Conference, San Antonio...

Gomez, J.

2013-01-01T23:59:59.000Z

470

Search for long-lived isomeric states in neutron-deficient thorium isotopes  

E-Print Network [OSTI]

The discovery of naturally occurring long-lived isomeric states (t_1/2 > 10^8 yr) in the neutron-deficient isotopes 211,213,217,218Th [A. Marinov et al., Phys. Rev. C 76, 021303(R) (2007)] was reexamined using accelerator mass spectrometry (AMS). Because AMS does not suffer from molecular isobaric background in the detection system, it is an extremely sensitive technique. Despite our up to two orders of magnitude higher sensitivity we cannot confirm the discoveries of neutron-deficient thorium isotopes and provide upper limits for their abundances.

J. Lachner; I. Dillmann; T. Faestermann; G. Korschinek; M. Poutivtsev; G. Rugel

2009-07-01T23:59:59.000Z

471

Upcoming Live Discussion on Energy 101: Fuel Cells | Department of Energy  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear SecurityTensile Strain Switched Ferromagnetism inS-4500II Field Emission SEM with EDAX (For3 MeetingLive Discussion

472

"Table HC13.2 Living Space Characteristics by South Census Region, 2005"  

U.S. Energy Information Administration (EIA) Indexed Site

AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data CenterFranconia, Virginia:FAQ < RAPID Jump to:SeadovCooperativeA2. World9, 2014 International PetroleumFuel Oil8Status2.9 Home2 Living

473

Pentanol isomer synthesis in engineered microorganisms  

E-Print Network [OSTI]

potential application as biofuels. They are found as natural3-Methyl-1-butanol . Biofuels 32 MJ/L for gasoline) and aabout microbial production of biofuels in general have been

Cann, Anthony F.; Liao, James C.

2010-01-01T23:59:59.000Z

474

Microorganism genomics, compositions and methods related thereto  

DOE Patents [OSTI]

The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

Handelsman, Jo (Madison, WI); Goodman, Robert M. (Madison, WI); Rondon, Michelle R. (Madison, WI)

2001-01-01T23:59:59.000Z

475

METHANE INCORPORATION BY PROCARYOTIC PHOTOSYNTHETIC MICROORGANISMS  

E-Print Network [OSTI]

blue-green alga. Low-density nuclear material is abundant inobtained from the New England Nuclear Corpor- ation (NEN) inin C H The New England Nuclear Corporation was unable to

Norton, Charles J.; Kirk, Martha; Calvin, Melvin

1970-01-01T23:59:59.000Z

476

Search for ancient microorganisms in Lake Baikal  

SciTech Connect (OSTI)

Lake Baikal in Russia, the world's oldest and deepest continental lake lies in south central Siberia, near the border to Mongolia. The lake is 1,643 m deep and has an area of about 46,000 km2. It holds one-fifth of all the terrestrial fresh water on Earth. Lake Baikal occupies the deepest portion of the Baikal Rift Zone. It was formed some 30-45 million years ago. The isolated Lake Baikal ecosystem represents a unique niche in nature based on its historical formation. The microbial diversity present in this environment has not yet been fully harvested or examined for products and processes of commercial interest and value. Thus, the collection of water, soil, and sub-bottom sediment samples was decided to characterize the microbial diversity of the isolated strains and to screen the isolates for their biotechnological value.

Hunter-Cevera, Jennie C.; Repin, Vladimir E.; Torok, Tamas

2000-06-14T23:59:59.000Z

477

Production of exotic, short lived carbon isotopes in ISOL-type facilities  

E-Print Network [OSTI]

The beam intensities of short-lived carbon isotopes at Isotope Separation On-Line (ISOL) facilities have been limited in the past for technical reasons. The production of radioactive ion beams of carbon isotopes is currently of high interest for fundamental nuclear physics research. To produce radioactive ions a target station consisting of a target in a container connected to an ion source via a transfer line is commonly used. The target is heated to vaporize the product for transport. Carbon in elementary form is a very reactive element and react strongly with hot metal surfaces. Due to the strong chemisorption interaction, in the target and ion source unit, the atoms undergo significant retention on their way from the target to the ion source. Due to this the short lived isotopes decays and are lost leading to low ion yields. A first approach to tackle these limitations consists of incorporating the carbon atoms into less reactive molecules and to use materials for the target housing and the transfer line ...

Franberg, Hanna; Köster, Ulli; Ammann, Markus

2008-01-01T23:59:59.000Z

478

Search for long-lived gravitational-wave transients coincident with long gamma-ray bursts  

E-Print Network [OSTI]

Long gamma-ray bursts (GRBs) have been linked to extreme core-collapse supernovae from massive stars. Gravitational waves (GW) offer a probe of the physics behind long GRBs. We investigate models of long-lived (~10-1000s) GW emission associated with the accretion disk of a collapsed star or with its protoneutron star remnant. Using data from LIGO's fifth science run, and GRB triggers from the swift experiment, we perform a search for unmodeled long-lived GW transients. Finding no evidence of GW emission, we place 90% confidence level upper limits on the GW fluence at Earth from long GRBs for three waveforms inspired by a model of GWs from accretion disk instabilities. These limits range from F<3.5 ergs cm^-2 to $F<1200 ergs cm^-2, depending on the GRB and on the model, allowing us to probe optimistic scenarios of GW production out to distances as far as ~33 Mpc. Advanced detectors are expected to achieve strain sensitivities 10x better than initial LIGO, potentially allowing us to probe the engines of the nearest long GRBs.

The LIGO Scientific Collaboration; the Virgo Collaboration; J. Aasi; J. Abadie; B. P. Abbott; R. Abbott; T. Abbott; M. R. Abernathy; T. Accadia; F. Acernese; C. Adams; T. Adams; R. X. Adhikari; C. Affeldt; M. Agathos; N. Aggarwal; O. D. Aguiar; P. Ajith; B. Allen; A. Allocca; E. Amador Ceron; D. Amariutei; R. A. Anderson; S. B. Anderson; W. G. Anderson; K. Arai; M. C. Araya; C. Arceneaux; J. Areeda; S. Ast; S. M. Aston; P. Astone; P. Aufmuth; C. Aulbert; L. Austin; B. E. Aylott; S. Babak; P. T. Baker; G. Ballardin; S. W. Ballmer; J. C. Barayoga; D. Barker; S. H. Barnum; F. Barone; B. Barr; L. Barsotti; M. Barsuglia; M. A. Barton; I. Bartos; R. Bassiri; A. Basti; J. Batch; J. Bauchrowitz; Th. S. Bauer; M. Bebronne; B. Behnke; M. Bejger; M. G. Beker; A. S. Bell; C. Bell; I. Belopolski; G. Bergmann; J. M. Berliner; D. Bersanetti; A. Bertolini; D. Bessis; J. Betzwieser; P. T. Beyersdorf; T. Bhadbhade; I. A. Bilenko; G. Billingsley; J. Birch; M. Bitossi; M. A. Bizouard; E. Black; J. K. Blackburn; L. Blackburn; D. Blair; M. Blom; O. Bock; T. P. Bodiya; M. Boer; C. Bogan; C. Bond; F. Bondu; L. Bonelli; R. Bonnand; R. Bork; M. Born; V. Boschi; S. Bose; L. Bosi; J. Bowers; C. Bradaschia; P. R. Brady; V. B. Braginsky; M. Branchesi; C. A. Brannen; J. E. Brau; J. Breyer; T. Briant; D. O. Bridges; A. Brillet; M. Brinkmann; V. Brisson; M. Britzger; A. F. Brooks; D. A. Brown; D. D. Brown; F. Br; T. Bulik; H. J. Bulten; A. Buonanno; D. Buskulic; C. Buy; R. L. Byer; L. Cadonati; G. Cagnoli; J. Calderon Bustillo; E. Calloni; J. B. Camp; P. Campsie; K. C. Cannon; B. Canuel; J. Cao; C. D. Capano; F. Carbognani; L. Carbone; S. Caride; A. Castiglia; S. Caudill; M. Cavagli; F. Cavalier; R. Cavalieri; G. Cella; C. Cepeda; E. Cesarini; R. Chakraborty; T. Chalermsongsak; S. Chao; P. Charlton; E. Chassande-Mottin; X. Chen; Y. Chen; A. Chincarini; A. Chiummo; H. S. Cho; J. Chow; N. Christensen; Q. Chu; S. S. Y. Chua; S. Chung; G. Ciani; F. Clara; D. E. Clark; J. A. Clark; F. Cleva; E. Coccia; P. -F. Cohadon; A. Colla; M. Colombini; M. Constancio Jr.; A. Conte; R. Conte; D. Cook; T. R. Corbitt; M. Cordier; N. Cornish; A. Corsi; C. A. Costa; M. W. Coughlin; J. -P. Coulon; S. Countryman; P. Couvares; D. M. Coward; M. Cowart; D. C. Coyne; K. Craig; J. D. E. Creighton; T. D. Creighton; S. G. Crowder; A. Cumming; L. Cunningham; E. Cuoco; K. Dahl; T. Dal Canton; M. Damjanic; S. L. Danilishin; S. D'Antonio; K. Danzmann; V. Dattilo; B. Daudert; H. Daveloza; M. Davier; G. S. Davies; E. J. Daw; R. Day; T. Dayanga; R. De Rosa; G. Debreczeni; J. Degallaix; W. Del Pozzo; E. Deleeuw; S. Deleglise; T. Denker; T. Dent; H. Dereli; V. Dergachev; R. DeRosa; R. DeSalvo; S. Dhurandhar; L. Di Fiore; A. Di Lieto; I. Di Palma; A. Di Virgilio; M. Diaz; A. Dietz; K. Dmitry; F. Donovan; K. L. Dooley; S. Doravari; M. Drago; R. W. P. Drever; J. C. Driggers; Z. Du; J. -C. Dumas; S. Dwyer; T. Eberle; M. Edwards; A. Effler; P. Ehrens; J. Eichholz; S. S. Eikenberry; G. EndrH; R. Essick; T. Etzel; K. Evans; M. Evans; T. Evans; M. Factourovich; V. Fafone; S. Fairhurst; Q. Fang; S. Farinon; B. Farr; W. Farr; M. Favata; D. Fazi; H. Fehrmann; D. Feldbaum; I. Ferrante; F. Ferrini; F. Fidecaro; L. S. Finn; I. Fiori; R. Fisher; R. Flaminio; E. Foley; S. Foley; E. Forsi; N. Fotopoulos; J. -D. Fournier; S. Franco; S. Frasca; F. Frasconi; M. Frede; M. Frei; Z. Frei; A. Freise; R. Frey; T. T. Fricke; P. Fritschel; V. V. Frolov; M. -K. Fujimoto; P. Fulda; M. Fyffe; J. Gair; L. Gammaitoni; J. Garcia; F. Garufi; N. Gehrels; G. Gemme; E. Genin; A. Gennai; L. Gergely; S. Ghosh; J. A. Giaime; S. Giampanis; K. D. Giardina; A. Giazotto; S. Gil-Casanova; C. Gill; J. Gleason; E. Goetz; R. Goetz; L. Gondan; G. Gonzalez; N. Gordon; M. L. Gorodetsky; S. Gossan; S. Go; R. Gouaty; C. Graef; P. B. Graff; M. Granata; A. Grant; S. Gras; C. Gray; R. J. S. Greenhalgh; A. M. Gretarsson; C. Griffo; P. Groot; H. Grote; K. Grover; S. Grunewald; G. M. Guidi; C. Guido; K. E. Gushwa; E. K. Gustafson; R. Gustafson; B. Hall; E. Hall; D. Hammer; G. Hammond; M. Hanke; J. Hanks; C. Hanna; J. Hanson; J. Harms; G. M. Harry; I. W. Harry; E. D. Harstad; M. T. Hartman; K. Haughian; K. Hayama; J. Heefner; A. Heidmann; M. Heintze; H. Heitmann; P. Hello; G. Hemming; M. Hendry; I. S. Heng; A. W. Heptonstall; M. Heurs; S. Hild; D. Hoak; K. A. Hodge; K. Holt; M. Holtrop; T. Hong; S. Hooper; T. Horrom; D. J. Hosken; J. Hough; E. J. Howell; Y. Hu; Z. Hua; V. Huang; E. A. Huerta; B. Hughey; S. Husa; S. H. Huttner; M. Huynh; T. Huynh-Dinh; J. Iafrate; D. R. Ingram; R. Inta; T. Isogai; A. Ivanov; B. R. Iyer; K. Izumi; M. Jacobson; E. James; H. Jang; Y. J. Jang; P. Jaranowski; F. Jimenez-Forteza; W. W. Johnson; D. Jones; D. I. Jones; R. Jones; R. J. G. Jonker; L. Ju; Haris K; P. Kalmus; V. Kalogera; S. Kandhasamy; G. Kang; J. B. Kanner; M. Kasprzack; R. Kasturi; E. Katsavounidis; W. Katzman; H. Kaufer; K. Kaufman

2013-12-09T23:59:59.000Z

479

Trapping and Assembly of Living Colloids at Water/Water Interfaces  

E-Print Network [OSTI]

We study the assembly of colloids in a two phase water-water system that provides an environment that can sustain bacteria, providing a new structure with rich potential to confine and structure living colloids. The water-water system, formed via phase separation of a casein and xanthan mixture, forms a 3-D structure of coexisting casein-rich and xanthan-rich phases. Fluorescent labelling and confocal microscopy reveal the attachment of these living colloids, including Escherichia coli and Pseudomonas aeruginosa, at the interface between the two phases. Inert colloids also become trapped at the interfaces, suggesting that the observed attachment can be attributed to capillarity. Over time, these structures coarsen and eventually degrade, illustrating the dynamic nature of these systems. This system lays the foundation for future studies of the interplay of physicochemical properties of the fluid interfaces and bulk phases and microbial responses they provoke to induce complex spatial organization, to study species which occupy distinct niches, and to optimize efficient microbial cross-feeding or protection from competitors.

Sarah D. Hann; Mark Goulian; Daeyeon Lee; Kathleen J. Stebe

2014-12-11T23:59:59.000Z

480

Short-lived pollutants in the Arctic: their climate impact and possible mitigation strategies  

SciTech Connect (OSTI)

Several short-lived pollutants known to impact Arctic climate may be contributing to the accelerated rates of warming observed in this region relative to the global annually averaged temperature increase. Here, we present a summary of the short-lived pollutants that impact Arctic climate including methane, tropospheric ozone, and tropospheric aerosols. For each pollutant, we provide a description of the major sources and the mechanism of forcing. We also provide the first seasonally averaged forcing and corresponding temperature response estimates focused specifically on the Arctic. The calculations indicate that the forcings due to black carbon, methane, and tropospheric ozone lead to a positive surface temperature response indicating the need to reduce emissions of these species within and outside the Arctic. Additional aerosol species may also lead to surface warming if the aerosol is coincident with thin, low lying clouds. We suggest strategies for reducing the warming based on current knowledge and discuss directions for future research to address the large remaining uncertainties.

Menon, Surabi; Quinn, P.K.; Bates, T.S.; Baum, E.; Doubleday, N.; Fiore, A.M.; Flanner, M.; Fridlind, A.; Garrett, T.J.; Koch, D.; Menon, S.; Shindell, D.; Stohl, A.; Warren, S.G.

2007-09-24T23:59:59.000Z

Note: This page contains sample records for the topic "microorganisms living active" from the National Library of EnergyBeta (NLEBeta).
While these samples are representative of the content of NLEBeta,
they are not comprehensive nor are they the most current set.
We encourage you to perform a real-time search of NLEBeta
to obtain the most current and comprehensive results.


481

Real-Time Gene Expression Profiling of Live Shewanella Oneidensis Cells  

SciTech Connect (OSTI)

The overall objective of this proposal is to make real-time observations of gene expression in live Shewanella oneidensis cells with high sensitivity and high throughput. Gene expression, a central process to all life, is stochastic because most genes often exist in one or two copies per cell. Although the central dogma of molecular biology has been proven beyond doubt, due to insufficient sensitivity, stochastic protein production has not been visualized in real time in an individual cell at the single-molecule level. We report the first direct observation of single protein molecules as they are generated, one at a time in a single live E. coli cell, yielding quantitative information about gene expression [Science 2006; 311: 1600-1603]. We demonstrated a general strategy for live-cell single-molecule measurements: detection by localization. It is difficult to detect single fluorescence protein molecules inside cytoplasm - their fluorescence is spread by fast diffusion to the entire cell and overwhelmed by the strong autofluorescence. We achieved single-molecule sensitivity by immobilizing the fluorescence protein on the cell membrane, where the diffusion is much slowed. We learned that under the repressed condition protein molecules are produced in bursts, with each burst originating from a stochastically-transcribed single messenger RNA molecule, and that protein copy numbers in the bursts follow a geometric distribution. We also simultaneously published a paper reporting a different method using ?-glactosidase as a reporter [Nature 440, 358 (2006)]. Many important proteins are expressed at low levels, inaccessible by previous proteomic techniques. Both papers allowed quantification of protein expression with unprecedented sensitivity and received overwhelming acclaim from the scientific community. The Nature paper has been identified as one of the most-cited papers in the past year [http://esi-topics.com/]. We have also an analytical framework describing the steady-state distribution of protein concentration in live cells, considering that protein production occurs in random bursts with an exponentially distributed number of molecules. This model allows for the extraction of kinetic parameters of gene expression from steady-state distributions of protein concentration in a cell population, which are available from single cell data obtained by fluorescence microscopy. [Phys. Rev. Lett. 97, 168302 (2006)]. A major objective in the Genome to Life (GtL) program is to monitor and understand the gene expression profile of a complete bacterial genome. We developed genetic and imaging methods for sensitive protein expression profiling in individual S. oneidensis cell. We have made good progress in constructing YFP-library with several hundred chromosomal fusion proteins and studied protein expression profiling in living Shewanella oneidensis cells. Fluorescence microscopy revealed the average abundance of specific proteins, as well as their noise in gene expression level across a population. We also explored ways to adapt our fluorescence measurement for other growth conditions, such as anaerobic growth.

Xiaoliang Sunney Xie

2009-03-30T23:59:59.000Z

482

Connecting local active forces to macroscopic stress in elastic media  

E-Print Network [OSTI]

In contrast with ordinary materials, living matter drives its own motion by generating active, out-of-equilibrium internal stresses. These stresses typically originate from localized active elements embedded in an elastic medium, such as molecular motors inside the cell or contractile cells in a tissue. While many large-scale phenomenological theories of such active media have been developed, a systematic understanding of the emergence of stress from the local force-generating elements is lacking. In this paper, we present a rigorous theoretical framework to study this relationship. We show that the medium's macroscopic active stress tensor is equal to the active elements' force dipole tensor per unit volume in both continuum and discrete linear homogeneous media of arbitrary geometries. This relationship is conserved on average in the presence of disorder, but can be violated in nonlinear elastic media. Such effects can lead to either a reinforcement or an attenuation of the active stresses, giving us a glimpse of the ways in which nature might harness microscopic forces to create active materials.

Pierre Ronceray; Martin Lenz

2014-11-12T23:59:59.000Z

483

Living in a Materials World: Materials Science Engineering Professional Development for K-12 Educators  

SciTech Connect (OSTI)

Advances in materials science are fundamental to technological developments and have broad societal impacs. For example, a cellular phone is composed of a polymer case, liquid crystal displays, LEDs, silicon chips, Ni-Cd batteries, resistors, capacitors, speakers, microphones all of which have required advances in materials science to be compacted into a phone which is typically smaller than a deck of cards. Like many technological developments, cellular phones have become a ubiquitous part of society, and yet most people know little about the materials science associated with their manufacture. The probable condition of constrained knowledge of materials science was the motivation for developing and offering a 20 hour fourday course called 'Living in a Materials World.' In addition, materials science provides a connection between our every day experiences and the work of scientists and engineers. The course was offered as part of a larger K-12 teacher professional development project and was a component of a week-long summer institute designed specifically for upper elementary and middle school teachers which included 20 hour content strands, and 12 hours of plenary sessions, planning, and collaborative sharing. The focus of the institute was on enhancing teacher content knowledge in STEM, their capacity for teaching using inquiry, their comfort and positive attitudes toward teaching STEM, their knowledge of how people learn, and strategies for integrating STEM throughout the curriculum. In addition to the summer institute the participating teachers were provided with a kit of about $300 worth of materials and equipment to use to implement the content they learned in their classrooms. As part of this professional development project the participants were required to design and implement 5 lesson plans with their students this fall and report on the results, as part of the continuing education course associated with the project. 'Living in a Materials World' was one of the fifteen content strands offered at the institute. The summer institute participants were pre/post tested on their comfort with STEM, their perceptions of STEM education, their pedagogical discontentment, their implementations of inquiry, their attitudes toward student learning of STEM, and their content knowledge associated with their specific content strand. The results from our research indicate a significant increase in content knowledge (t = 11.36, p < .01) for the Living in a Materials World strand participants. Overall the summer institute participants were found to have significant increases in their comfort levels for teaching STEM (t = 10.94, p < .01), in inquiry implementation (t = 5.72, p < .01) and efficacy for teaching STEM (t = 6.27, p < .01) and significant decrease in pedagogical discontentment (t = -6.26, p < .01).

Anne Seifert; Louis Nadelson

2011-06-01T23:59:59.000Z

484

Testing, Modeling, and Monitoring to Enable Simpler, Cheaper, Longer-lived Surface Caps  

SciTech Connect (OSTI)

Society has and will continue to generate hazardous wastes whose risks must be managed. For exceptionally toxic, long-lived, and feared waste, the solution is deep burial, e.g., deep geological disposal at Yucca Mtn. For some waste, recycle or destruction/treatment is possible. The alternative for other wastes is storage at or near the ground level (in someone's back yard); most of these storage sites include a surface barrier (cap) to prevent downward water migration. Some of the hazards will persist indefinitely. As society and regulators have demanded additional proof that caps are robust against more threats and for longer time periods, the caps have become increasingly complex and expensive. As in other industries, increased complexity will eventually increase the difficulty in estimating performance, in monitoring system/component performance, and in repairing or upgrading barriers as risks are managed. An approach leading to simpler, less expensive, longer-lived, more manageable caps is needed. Our project, which started in April 2002, aims to catalyze a Barrier Improvement Cycle (iterative learning and application) and thus enable Remediation System Performance Management (doing the right maintenance neither too early nor too late). The knowledge gained and the capabilities built will help verify the adequacy of past remedial decisions, improve barrier management, and enable improved solutions for future decisions. We believe it will be possible to develop simpler, longer-lived, less expensive caps that are easier to monitor, manage, and repair. The project is planned to: (a) improve the knowledge of degradation mechanisms in times shorter than service life; (b) improve modeling of barrier degradation dynamics; (c) develop sensor systems to identify early degradation; and (d) provide a better basis for developing and testing of new barrier systems. This project combines selected exploratory studies (benchtop and field scale), coupled effects accelerated aging testing at the intermediate meso-scale, testing of new monitoring concepts, and modeling of dynamic systems. The emphasis on meso-scale (coupled) tests, accelerated effects testing, and dynamic modeling differentiates the project from other efforts, while simultaneously building on that body of knowledge. The performance of evapotranspiration, capillary, and grout-based barriers is being examined. To date, the project can report new approaches to the problem, building new experimental and modeling capabilities, and a few preliminary results.

Piet, S. J.; Breckenridge, R. P.; Burns, D. E.

2003-02-25T23:59:59.000Z

485

Testing, Modeling, and Monitoring to Enable Simpler, Cheaper, Longer-Lived Surface Caps  

SciTech Connect (OSTI)

Society has and will continue to generate hazardous wastes whose risks must be managed. For exceptionally toxic, long-lived, and feared waste, the solution is deep burial, e.g., deep geological disposal at Yucca Mtn. For some waste, recycle or destruction/treatment is possible. The alternative for other wastes is storage at or near the ground level (in someone’s back yard); most of these storage sites include a surface barrier (cap) to prevent downward water migration. Some of the hazards will persist indefinitely. As society and regulators have demanded additional proof that caps are robust against more threats and for longer time periods, the caps have become increasingly complex and expensive. As in other industries, increased complexity will eventually increase the difficulty in estimating performance, in monitoring system/component performance, and in repairing or upgrading barriers as risks are managed. An approach leading to simpler, less expensive, longer-lived, more manageable caps is needed. Our project, which started in April 2002, aims to catalyze a Barrier Improvement Cycle (iterative learning and application) and thus enable Remediation System Performance Management (doing the right maintenance neither too early nor too late). The knowledge gained and the capabilities built will help verify the adequacy of past remedial decisions, improve barrier management, and enable improved solutions for future decisions. We believe it will be possible to develop simpler, longer-lived, less expensive caps that are easier to monitor, manage, and repair. The project is planned to: a) improve the knowledge of degradation mechanisms in times shorter than service life; b) improve modeling of barrier degradation dynamics; c) develop sensor systems to identify early degradation; and d) provide a better basis for developing and testing of new barrier systems. This project combines selected exploratory studies (benchtop and field scale), coupled effects accelerated aging testing at the intermediate meso-scale, testing of new monitoring concepts, and modeling of dynamic systems. The emphasis on meso-scale (coupled) tests, accelerated effects testing, and dynamic modeling differentiates the project from other efforts, while simultaneously building on that body of knowledge. The performance of evapotranspiration, capillary, and grout-based barriers is being examined. To date, the project can report new approaches to the problem, building new experimental and modeling capabilities, and a few preliminary results.

Piet, Steven James; Breckenridge, Robert Paul; Burns, Douglas Edward

2003-02-01T23:59:59.000Z

486

and solar activity  

E-Print Network [OSTI]

Abstract. Solar activity, together with human activity, is considered a possible factor for the global warming observed in the last century. However, in the last decades solar activity has remained more or less constant while surface air temperature has continued to increase, which is interpreted as an evidence that in this period human activity is the main factor for global warming. We show that the index commonly used for quantifying long-term changes in solar activity, the sunspot number, accounts for only one part of solar activity and using this index leads to the underestimation of the role of solar activity in the global warming in the recent decades. A more suitable index is the geomagnetic activity which reflects all solar activity, and it is highly correlated to global temperature variations in the whole period for which we have data. Key words. Solar activity, Global warming 1. Sunspot number and global temperature The most popular index of solar activity is the International sunspot number (R). A reconstruction

Memorie Della; K. Georgieva; C. Bianchi; B. Kirov

487

Status of delayed-neutron precursor data: Half-lives and neutron emission probabilities  

E-Print Network [OSTI]

We present in this paper a compilation of the present status of experimental delayed-neutron precursor data; i.e. beta-decay half-lives (T_1/2) and neutron emission probabilities (P_n) in the fission-product region (27 <= Z <= 57). These data are compared to two model predictions of substantially different sophistication: (i) an update of the empirical Kratz-Herrmann formula (KHF), and (ii) a unified macroscopic-microscopic model within the quasi-particle random-phase approximation (QRPA). Both models are also used to calculate so far unknown T_1/2 and P_n values up to Z=63. A number of possible refinements in the microscopic calculations are suggested to further improve the nuclear-physics foundation of these data for reactor and astrophysical applications.

B. Pfeiffer; K. -L. Kratz; P. Moeller

2001-06-22T23:59:59.000Z

488

Estimations of local thermal impact on living organisms irradiated by non-thermal microwaves  

E-Print Network [OSTI]

Pennes' differential equation for bioheat transfer and the heat transfer equation are solved for the temperature distribution in a living tissue with spherical inclusions, irradiated by microwave power. It is shown that relative temperature excess in a small inclusion in the tissue in some cases is inversely proportional to its radius and does not depend on the applied power. In pulsing RF fields the effect is amplified proportionally to the ratio of the pulse period to the pulse duration. The local temperature rise significantly outpaces the averaged one and therefore the Watt to Weight SAR limits may be insufficient to estimate the safety of RF radiation and the conventional division of the biological effects of electromagnetic fields on the thermal and non-thermal needs to be revised.

Shatalov, Vladimir

2013-01-01T23:59:59.000Z

489

Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation  

SciTech Connect (OSTI)

Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

Fedotov, I. V.; Doronina-Amitonova, L. V. [International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation); Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 1430125 (Russian Federation); Kurchatov Institute National Research Center, Moscow (Russian Federation); Sidorov-Biryukov, D. A.; Fedotov, A. B. [International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation); Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 1430125 (Russian Federation); Anokhin, K. V. [Kurchatov Institute National Research Center, Moscow (Russian Federation); P.K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow (Russian Federation); Kilin, S. Ya. [B.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, Minsk (Belarus); Sakoda, K. [National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan); Zheltikov, A. M. [International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation); Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 1430125 (Russian Federation); Department of Physics and Astronomy, Texas A and M University, College Station, Texas 77843 (United States); Center of Photochemistry, Russian Academy of Sciences, ul. Novatorov 7a, Moscow 117421 (Russian Federation)

2014-02-24T23:59:59.000Z

490

Removal of long-lived $^{222}$Rn daughters by electropolishing thin layers of stainless steel  

E-Print Network [OSTI]

Long-lived alpha and beta emitters in the $^{222}$Rn decay chain on detector surfaces may be the limiting background in many experiments attempting to detect dark matter or neutrinoless double-beta decay. Removal of tens of microns of material via electropolishing has been shown to be effective at removing radon daughters implanted into material surfaces. Some applications, however, require the removal of uniform and significantly smaller thicknesses. Here, we demonstrate that electropolishing steel plates reduces the contamination efficiently, by a factor > 100. Examination of electropolished wires with a scanning electron microscope confirms that the thickness removed is reproducible and reasonably uniform. Together, these tests demonstrate the effectiveness of removal of radon daughters for a proposed low-radiation, multi-wire proportional chamber (the BetaCage), without compromising the screener's energy resolution. More generally, electropolishing thin layers of stainless steel may effectively remove radon daughters without compromising precision-machined parts.

R. W. Schnee; M. A. Bowles; R. Bunker; K. McCabe; J. White; P. Cushman; M. Pepin; V. E. Guiseppe

2014-04-23T23:59:59.000Z

491

Removal of long-lived {sup 222}Rn daughters by electropolishing thin layers of stainless steel  

SciTech Connect (OSTI)

Long-lived alpha and beta emitters in the {sup 222}Rn decay chain on detector surfaces may be the limiting background in many experiments attempting to detect dark matter or neutrinoless double beta decay. Removal of tens of microns of material via electropolishing has been shown to be effective at removing radon daughters implanted into material surfaces. Some applications, however, require the removal of uniform and significantly smaller thicknesses. Here, we demonstrate that electropolishing < 1 ?m from stainless-steel plates reduces the contamination efficiently, by a factor > 100. Examination of electropolished wires with a scanning electron microscope confirms that the thickness removed is reproducible and reasonably uniform. Together, these tests demonstrate the effectiveness of removal of radon daughters for a proposed low-radiation, multi-wire proportional chamber (the BetaCage), without compromising the screener’s energy resolution. More generally, electropolishing thin layers of stainless steel may effectively remove radon daughters without compromising precision-machined parts.

Schnee, R. W.; Bowles, M. A.; Bunker, R.; McCabe, K.; White, J. [Department of Physics, Syracuse University, Syracuse, NY 13244 (United States)] [Department of Physics, Syracuse University, Syracuse, NY 13244 (United States); Cushman, P.; Pepin, M. [School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 (United States)] [School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455 (United States); Guiseppe, V. E. [University of South Dakota, Vermillion, South Dakota 57069 (United States)] [University of South Dakota, Vermillion, South Dakota 57069 (United States)

2013-08-08T23:59:59.000Z

492

A New Approach to the Living Anionic Polymerization of 4-Cyanostyrene  

SciTech Connect (OSTI)

A new approach to the synthesis of well-defined homopolymers of 4-cyanostyrene (4CNS) via anionic polymerization using high vacuum techniques is described. This synthetic route is based on the use of a solvent mixture of tetrahydrofuran and N,N-dimethylacetamide (THF/DMA, 4/1 v/v) in order to overcome the previously reported polymer insolubility issue and long reaction times. Our system afforded well-defined linear living P4CNSs having molecular weights up to 98,500 g/mol in less than 30 min and can be applied to the synthesis of P4CNS-based homopolymers and copolymers of various molecular architectures.

Driva, Paraskevi [ORNL; Pickel, Deanna L [ORNL; Mays, Jimmy [ORNL; Baskaran, Durairaj [University of Tennessee, Knoxville (UTK)

2010-01-01T23:59:59.000Z

493

Detecting very long-lived gravitational-wave transients lasting hours to weeks  

E-Print Network [OSTI]

We explore the possibility of very long-lived gravitational-wave transients (and detector artifacts) lasting hours to weeks. Such very long signals are both interesting in their own right and as a potential source of systematic error in searches for persistent signals, e.g., from a stochastic gravitational-wave background. We review possible mechanisms for emission on these time scales and discuss computational challenges associated with their detection: namely, the substantial volume of data involved in a search for very long transients can require vast computer memory and processing time. These computational difficulties can be addressed through a form of data compression known as coarse-graining, in which information about short time spans is discarded in order to reduce the computational requirements of a search. Using data compression, we demonstrate an efficient radiometer (cross-correlation) algorithm for the detection of very long transients. In the process, we identify features of a very long transie...

Thrane, Eric; Christensen, Nelson

2015-01-01T23:59:59.000Z

494

Experimental neutronics tests for a neutron activation system for the European ITER TBM  

SciTech Connect (OSTI)

We are investigating methods for neutron flux measurement in the ITER TBM. In particular we have tested sets of activation materials leading to induced gamma activities with short half-lives of the order of tens of seconds up to minutes and standard activation materials. Packages of activation foils have been irradiated with the intense neutron generator of Technical University of Dresden in a pure DT neutron field as well as in a neutronics mock-up of the European ITER HCLL TBM. An important aim was to check whether the gamma activity induced in the activation foils in these packages could be measured simultaneously. It was indeed possible to identify gamma lines of interest in gamma-ray measurements immediately after extraction from the irradiation.

Klix, A.; Fischer, U. [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany); Gehre, D. [Technical University of Dresden, IKTP, Zellescher Weg 19, 01062 Dresden (Germany); Kleizer, G. [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Budapest University of Technology and Economics, M?egyetem rkp. 3-9. H-1111 Budapest (Hungary); Raj, P. [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Université Paris-Sud, 15 rue Georges Clemenceau, F-91405 Paris (France); Rovni, I. [Budapest University of Technology and Economics, M?egyetem rkp. 3-9. H-1111 Budapest (Hungary); Ruecker, Tom [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and University of Applied Sciences Zittau-Goerlitz, Theodor-Körner-Allee 16, D-02754 Zittau (Germany)

2014-08-21T23:59:59.000Z

495

Realistic fission models, new beta-decay half-lives and the r-process in neutron star mergers  

SciTech Connect (OSTI)

Almost half of heavy nuclei beyond iron are considered to be produced by rapid neutron capture process (r-process). This process occurs in the neutron-rich environment such as core-collapse supernovae or neutron star mergers, but the main production site is still unknown. In the r-process of neutron star mergers, nuclear fission reactions play an important role. Also beta-decay half-lives of magic nuclei are crucial for the r-process. We have carried out r-process nucleosynthesis calculations based upon new theoretical estimates of fission fragment distributions and new beta-decay half-lives for N=82 nuclei measured at RIBF-RIKEN. We investigate the effect of nuclear fission on abundance patterns in the matter ejected from neutron star mergers with two different fission fragment mass distributions. We also discuss how the new experimental beta-decay half-lives affect the r-process.

Shibagaki, S.; Kajino, T. [Department of Astronomy, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Chiba, S. [Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, 152-8850 (Japan); Lorusso, G.; Nishimura, S. [RIKEN Nishina Center, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan); Mathews, G. J. [Center for Astrophysics, Department of Physics, University of Notre Dame, IN 46556 (United States)

2014-05-02T23:59:59.000Z

496

Half-lives and delayed neutron emission probabilities of neutron-rich Li--Al nuclides (US)  

SciTech Connect (OSTI)

Beta-decay half-lives and delayed neutron emission probabilities of very neutron-rich nuclides from Li to Al have been measured by a delayed coincidence technique. Neutron-rich nuclides were produced by reaction of 800-MeV protons on a {sup 232}Th target and were individually identified by use of the time-of-flight isochronous spectrometer. For 16 previously measured nucides, the half-lives determined here agree with literature values within the uncertainties. The previously unmeasured half-lives for {sup 25}F (59{plus minus}40 ms) and {sup 28}Ne (14{plus minus}10 ms) have been determined. Delayed neutron emission has been measured for {sup 12}Be, {sup 14}B, {sup 17}C, {sup 18}N, {sup 25}F, and {sup 28}Ne. Emission probabilities are reported for 19 precursors and upper limits are reported for 12 other nuclides. These data are compared with predictions of several beta-decay models.

Reeder, P.L.; Warner, R.A.; Hensley, W.K. (Pacific Northwest Laboratory, Richland, Washington (USA)); Vieira, D.J.; Wouters, J.M. (Los Alamos National Laboratory, Los Alamos, New Mexico (USA))

1991-10-01T23:59:59.000Z

497

Cosmogenic activation of a natural tellurium target  

E-Print Network [OSTI]

130Te is one of the candidates for the search for neutrinoless double beta decay. It is currently planned to be used in two experiments: CUORE and SNO+. In the CUORE experiment TeO2 crystals cooled at cryogenic temperatures will be used. In the SNO+ experiment natTe will be deployed up to 0.3% loading in the liquid scintillator volume. A possible background for the signal searched for, are the high Q-value, long-lived isotopes, produced by cosmogenic neutron and proton spallation reaction on the target material. A total of 18 isotopes with Q-value larger than 2 MeV and T1/2 >20 days have been identified as potential backgrounds. In addition low Q-value, high rate isotopes can be problematic due to pile-up effects, specially in liquid scintillator based detectors. Production rates have been calculated using the ACTIVIA program, the TENDL library, and the cosmogenic neutron and proton flux parametrization at sea level from Armstrong and Gehrels for both long and short lived isotopes. The obtained values for the cross sections are compared with the existing experimental data and calculations. Good agreement has been generally found. The results have been applied to the SNO+ experiment for one year of exposure at sea level. Two possible cases have been considered: a two years of cooling down period deep underground, or a first purification on surface and 6 months of cooling down deep underground. Deep underground activation at the SNOLAB location has been considered.

V. Lozza; J. Petzoldt

2014-11-21T23:59:59.000Z

498

Role of Extracellular Polymeric Substances in Bioflocculation...  

Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

Microorganisms under GlucoseControlled Abstract: Extracellular polymeric substances (EPS) secreted by suspended cultures of microorganisms from an activated sludge plant in the...

499

Activated carbon aerogels  

SciTech Connect (OSTI)

Activated carbon aerogels were obtained from the CO{sub 2} activation of the carbon aerogels. The adsorption isotherms of nitrogen on activated carbon aerogels at 77 K were measured and analyzed by the high-resolution {alpha}{sub s} plot to evaluate their porosities. The {alpha}{sub s} plot showed an upward deviation from linearity below {alpha}{sub s} = 0.5, suggesting that the presence of micropores becomes more predominant with the extent of the activation. Activation increased noticeably the pore volume and the surface area (the maximum value: 2600 m{sup 2}.g{sup -1}) without change of the basic network structure of primary particles. Activated carbon aerogels had a bimodal pore size distribution of uniform micropores and mesopores. 16 refs., 2 figs., 1 tab.

Hanzawa, Y.; Kaneko, K. [Chiba Univ. (Japan)] [Chiba Univ. (Japan); Pekala, R.W. [Lawrence Livermore National Lab., CA (United States)] [Lawrence Livermore National Lab., CA (United States); Dresselhaus, M.S. [Massachusetts Inst. of Technology, Cambridge, MA (United States)] [Massachusetts Inst. of Technology, Cambridge, MA (United States)

1996-12-25T23:59:59.000Z

500

Activated recombinant adenovirus proteinases  

DOE Patents [OSTI]

This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described. 29 figs.

Anderson, C.W.; Mangel, W.F.

1999-08-10T23:59:59.000Z