Sample records for microorganisms living active

  1. Studies on the activities of rumen microorganisms in vitro

    E-Print Network [OSTI]

    Johnson, Carl Edward

    1957-01-01T23:59:59.000Z

    STCDIES ON THE ACTIVITIES OF RUMEN MICROORGANISMS IN VITRO A Thesis Carl~Johnson Submitted to the, Gr;. dusts School of the Agricultural and Mec?anical Co13 cgs oi' Texas in partial fulfillment of the requirements for the degree of MAST'% OS... SCIENCE August 1)57 Major Subject: Animal Nutrition STUDIES CN THE ACTIVITIES OF RUNEN MICROORGANISMS IN VITRO A Thesis Carl Johnson Approved as to style and oontent b Cha of Committee Head of Department August 1957 ACIQIOWLEDGMENTS The author...

  2. A laboratory study of the activities of dried rumen microorganisms

    E-Print Network [OSTI]

    Harbers, Leniel Henry

    1958-01-01T23:59:59.000Z

    . cal pro? ceases vere made in this study to determine the ~ ~ activity of dried rumen microorganisms. . 'ellulose d1- gestion vas studied by employing a yurified cellulose preparation as a substrate and determination of the changes in concentration... the conditions of these experiments. There vere~ hovevsr~ bacteria present in the dr1ed rumen preparations vhich vere capable of hydro- lysing carboxymethyl cellulose. A method of detsrming "cellulose d1gestion" using a soluble cellulose derivative as a...

  3. active living environment: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and physical activity environment audit tools Keinan, Alon 146 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  4. Energetics of active fluctuations in living cells

    E-Print Network [OSTI]

    Étienne Fodor; Kiyoshi Kanazawa; Hisao Hayakawa; Paolo Visco; Frédéric van Wijland

    2014-06-06T23:59:59.000Z

    The nonequilibrium activity taking place in a living cell can be monitored with a tracer embedded in the medium. While microrheology experiments based on optical manipulation of such probes have become increasingly standard, we put forward a number of experiments with alternative protocols that, we claim, will provide new insight into the energetics of active fluctuations. These are based on either performing thermodynamic--like cycles in control-parameter space, or on determining response to external perturbations of the confining trap beyond simple translation. We illustrate our proposals on an active itinerant Brownian oscillator modeling the dynamics of a probe embedded in a living medium.

  5. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30T23:59:59.000Z

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  6. The Characterization of Psychrophilic Microorganisms and their potentially useful Cold-Active Glycosidases Final Progress Report

    SciTech Connect (OSTI)

    Brenchly, Jean E.

    2008-06-30T23:59:59.000Z

    Our studies of novel, cold-loving microorganisms have focused on two distinct extreme environments. The first is an ice core sample from a 120,000 year old Greenland glacier. The results of this study are particularly exciting and have been highlighted with press releases and additional coverage. The first press release in 2004 was based on our presentation at the General Meeting of the American Society for Microbiology and was augmented by coverage of our publication (Appl. Environ. Microbiol. 2005. Vol. 71:7806) in the Current Topics section of the ASM news journal, “Microbe.” Of special interest for this report was the isolation of numerous, phylogenetically distinct and potentially novel ultrasmall microorganisms. The detection and isolation of members of the ultrasmall population is significant because these cells pass through 0.2 micron pore filters that are generally used to trap microorganisms from environmental samples. Thus, analyses by other investigators that examined only cells captured on the filters would have missed a significant portion of this population. Only a few ultrasmall isolates had been obtained prior to our examination of the ice core samples. Our development of a filtration enrichment and subsequent cultivation of these organisms has added extensively to the collection of, and knowledge about, this important population in the microbial world.

  7. active living logan: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 126 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  8. active living principles: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    theory of generalized functions. Vernaeve, Hans 2011-01-01 235 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  9. active living projects: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Brian Meyers; John Krumm; A Kern; Steven Shafer 2000-01-01 236 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  10. active living study: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Epstein, and Li Deng) and a computational Snider, Barry B. 215 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  11. Long-lived activation products in reactor materials

    SciTech Connect (OSTI)

    Evans, J.C.; Lepel, E.L.; Sanders, R.W.; Wilkerson, C.L.; Silker, W.; Thomas, C.W.; Abel, K.H.; Robertson, D.R.

    1984-08-01T23:59:59.000Z

    The purpose of this program was to assess the problems posed to reactor decommissioning by long-lived activation products in reactor construction materials. Samples of stainless steel, vessel steel, concrete, and concrete ingredients were analyzed for up to 52 elements in order to develop a data base of activatable major, minor, and trace elements. Large compositional variations were noted for some elements. Cobalt and niobium concentrations in stainless steel, for example, were found to vary by more than an order of magnitude. A thorough evaluation was made of all possible nuclear reactions that could lead to long lived activation products. It was concluded that all major activation products have been satisfactorily accounted for in decommissioning planning studies completed to date. A detailed series of calculations was carried out using average values of the measured compositions of the appropriate materials to predict the levels of activation products expected in reactor internals, vessel walls, and bioshield materials for PWR and BWR geometries. A comparison is made between calculated activation levels and regulatory guidelines for shallow land disposal according to 10 CFR 61. This analysis shows that PWR and BWR shroud material exceeds the Class C limits and is, therefore, generally unsuitable for near-surface disposal. The PWR core barrel material approaches the Class C limits. Most of the remaining massive components qualify as either Class A or B waste with the bioshield clearly Class A, even at the highest point of activation. Selected samples of activated steel and concrete were subjected to a limited radiochemical analysis program as a verification of the computer model. Reasonably good agreement with the calculations was obtained where comparison was possible. In particular, the presence of /sup 94/Nb in activated stainless steel at or somewhat above expected levels was confirmed.

  12. Supervised Classification of Activities of Daily Living in Health Smart Homes using SVM

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Supervised Classification of Activities of Daily Living in Health Smart Homes using SVM Anthony) on real data. Index Terms--Support vector machines, Activities of Daily Living, Health Smart Homes, Sensor smart homes to achieve this goal [1]. Several solutions are stud- ied by laboratories and companies

  13. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian; Kleff, Susanne; Guettler, Michael V

    2013-04-30T23:59:59.000Z

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  14. Recombinant microorganisms for increased production of organic acids

    DOE Patents [OSTI]

    Yi, Jian (East Lansing, MI); Kleff, Susanne (East Lansing, MI); Guettler, Michael V. (Holt, MI)

    2012-02-21T23:59:59.000Z

    Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

  15. Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation

    E-Print Network [OSTI]

    Craik, Charles S.

    Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of ``plasmon rulers'' to observe conformational

  16. Engineered microorganisms capable of producing target compounds under anaerobic conditions

    DOE Patents [OSTI]

    Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

    2012-01-17T23:59:59.000Z

    The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

  17. Activity-Based Prototyping of Ubicomp Applications for Long-Lived, Everyday Human Activities

    E-Print Network [OSTI]

    Anderson, Richard

    design process, and it allowed creating realistic ubicomp application prototypes at a low cost computing (ubicomp) promises to support our everyday activities by weaving computing power into the fabric by orienting designs towards human needs. ACD, however, is largely a set of perspectives and concepts

  18. Journal of Food,Agriculture & Environment, Vol.7 (2),April 2009 103 Antimicrobial activity against pathogenic microorganisms by extracts from herbal

    E-Print Network [OSTI]

    Khalil, Amjad

    Journal of Food,Agriculture & Environment, Vol.7 (2),April 2009 103 Antimicrobial activity against-fungal activity. www.world-food.netJournal of Food, Agriculture & Environment Vol.7 (2) : 103-106. 2009

  19. "A strong mind in a sane body": the role of physical activity in the lives and writings of Sophia Hawthorne, Margaret Fuller, and Kate Chopin

    E-Print Network [OSTI]

    Willoughby, Leann Miller

    1992-01-01T23:59:59.000Z

    "A STRONG MIND IN A SANE BODY": THE ROLE OF PHYSICAL ACTIVITY IN THE LIVES AND WRITINGS OF SOPHIA HAWTHORNE~ MARGARET FULLER, AND KATE CHOPIN A Thesis by LEANN MILLER WILLOUGHBY Submitted to the Office of Graduate Studies of Texas ASM... University in partial fulfillment of the requirements for the degree of MASTER OF ARTS August 1992 Major Subject: English ?A STRONG MIND IN A SANE BODY? . THE ROLE OF PHYSICAL ACTIVITY IN THE LIVES AND WRITINGS OF SOPHIA HAWTHORNE, MARGARET FULLER...

  20. A combined microfluidic/dielectrophoretic microorganism concentrator

    E-Print Network [OSTI]

    Gadish, Nitzan

    2005-01-01T23:59:59.000Z

    This thesis presents the development of a high-throughput microfluidic microorganism concentrator for pathogen detection applications. Interdigitated electrodes lining the bottom of the channel use positive dielectrophoretic ...

  1. Bacteriocins of Aquatic Microorganisms and Their Potential

    E-Print Network [OSTI]

    Riley, Margaret

    , known as pyocins (Govan & Harris, 1985). In contrast, only 15-50% of Escherichia coli produce from marine microorganisms. These toxins have received far less attention than bacteriocins produced

  2. Living Sustainably

    E-Print Network [OSTI]

    Milbrath, Lester W.

    1998-01-01T23:59:59.000Z

    sustainable society does something more than keep people alive; livingsustainable modes of behavior that also lead to quality in living.

  3. Neuroticism, Social Support and Activities of Daily Living in the Health Longevity Survey of the Old in China 

    E-Print Network [OSTI]

    Wu, Na

    2010-11-24T23:59:59.000Z

    mediator. Therefore, this study examined these findings for older people in China. Based on Chinese Longitudinal Health Longevity Survey dataset, 11,147 participants living in 22 of the 31 provinces in China were interviewed in 2000 by self...

  4. Nearby Outdoor Environmental Support of Older Adults' Yard Activities, Neighborhood Walking and Independent Living in the Community

    E-Print Network [OSTI]

    Wang, Zhe

    2011-02-22T23:59:59.000Z

    to remain healthy and gives them the ability to access daily-life services, and thus extend their independent years at home. Nearby outdoor environments on residential sites and in the neighborhoods may influence older adults' independent living through...

  5. Results an data on the growth of the microorganisms

    SciTech Connect (OSTI)

    Laurinavichius, K.S.

    1995-10-09T23:59:59.000Z

    The study of biocorrosion of aluminum and its alloy was performed under conditions of continuous fermentation of thermophilic anaerobic microorganisms of different groups. This allowed us to examine the effect of various types of metabolic reactions of reduction-oxidation proceeding at different pH and temperatures under highly reduced conditions on aluminum corrosion. Besides, the experiments were performed where the part of the standard sample was exposed under strictly anaerobic conditions with an active microbiological process, and the second half was exposed under aerobic conditions. Thus the sample was exposed in gradient of oxidized-reduced conditions.

  6. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06T23:59:59.000Z

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  7. Recent activities for ?-decay half-lives and ?-delayed neutron emission of very neutron-rich isotopes

    SciTech Connect (OSTI)

    Dillmann, Iris [TRIUMF, Vancouver BC, V6T 2A3, Canada and GSI Helmholtzzentrum für Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Abriola, Daniel [Laboratorio Tandar, Comisión Nacional de Energía Atómica, B1650KINA, San Martín, Buenos Aires (Argentina); Singh, Balraj [Department of Physics and Astronomy, McMaster University, Hamilton ON, L8S 4M1 (Canada)

    2014-05-02T23:59:59.000Z

    Beta-delayed neutron (?n) emitters play an important, two-fold role in the stellar nucleosynthesis of heavy elements in the 'rapid neutron-capture process' (r process). On one hand they lead to a detour of the material ?-decaying back to stability. On the other hand, the released neutrons increase the neutron-to-seed ratio, and are re-captured during the freeze-out phase and thus influence the final solar r-abundance curve. A large fraction of the isotopes inside the r-process reaction path are not yet experimentally accessible and are located in the (experimental) 'Terra Incognita'. With the next generation of fragmentation and ISOL facilities presently being built or already in operation, one of the main motivation of all projects is the investigation of these very neutron-rich isotopes. A short overview of one of the planned programs to measure ?n-emitters at the limits of the presently know isotopes, the BRIKEN campaign (Beta delayed neutron emission measurements at RIKEN) will be given. Presently, about 600 ?-delayed one-neutron emitters are accessible, but only for a third of them experimental data are available. Reaching more neutron-rich isotopes means also that multiple neutron-emission becomes the dominant decay mechanism. About 460 ?-delayed two-, three-or four-neutron emitters are identified up to now but for only 30 of them experimental data about the neutron branching ratios are available, most of them in the light mass region below A=30. The International Atomic and Energy Agency (IAEA) has identified the urgency and picked up this topic recently in a 'Coordinated Research Project' on a 'Reference Database for Beta-Delayed Neutron Emission Data'. This project will review, compile, and evaluate the existing data for neutron-branching ratios and half-lives of ?-delayed neutron emitters and help to ensure a reliable database for the future discoveries of new isotopes and help to constrain astrophysical and theoretical models.

  8. Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)

    SciTech Connect (OSTI)

    Ann M. Stock

    2009-04-08T23:59:59.000Z

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

  9. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let us countLightingFebruaryandFor Users Live

  10. Deciphering Active Estrogen-Degrading Microorganisms in Bioreactors

    E-Print Network [OSTI]

    Roh, Hyung Keun

    2010-10-12T23:59:59.000Z

    in biological wastewater treatment processes. This dissertation investigated factors affecting estrogen biodegradation in bioreactors. Specifically, research efforts were placed on characterization of several bacterial estrogen degraders (model strains...

  11. activated sludge microorganisms: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Samples of wastewater were collected from Aslogy wastewater treatment plant, Zagazig, Egypt. The using bacteria embed on granular delivered as powder. Two runs of experimental...

  12. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, J.L.; Clausen, E.C.

    1992-12-22T23:59:59.000Z

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

  13. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

    1992-01-01T23:59:59.000Z

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

  14. Selective microorganism concentration using a dielectrophoresis-based microfabricated device

    E-Print Network [OSTI]

    Pucha?a, Katarzyna Anna

    2007-01-01T23:59:59.000Z

    Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

  15. Microorganisms having enhanced tolerance to inhibitors and stress

    DOE Patents [OSTI]

    Brown, Steven D.; Yang, Shihui

    2014-07-29T23:59:59.000Z

    The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

  16. Microorganisms having enhanced resistance to acetate and methods of use

    DOE Patents [OSTI]

    Brown, Steven D; Yang, Shihui

    2014-10-21T23:59:59.000Z

    The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

  17. (Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)

    SciTech Connect (OSTI)

    Arp, D.J.

    1990-01-01T23:59:59.000Z

    Hydrogenases are enzymes which catalyze reactions involving dihydrogen. They serve integral roles in a number of microbial metabolic pathways. Our research is focussed on investigations of the catalytic mechanism of the hydrogenases found in aerobic, N{sub 2}-fixing microorganisms such as Azotobacter vinelandii and the agronomically important Bradyrhizobium japonicum as well as microorganisms with similar hydrogenases. The hydrogenases isolated from these microorganisms are Ni- and Fe-containing heterodimers. Our work has focussed on three areas during the last grant period. In all cases, a central theme has been the role of inhibitors in the characteristics under investigation. In addition, a number of collaborative efforts have yielded interesting results. In metalloenzymes such as hydrogenase, inhibitors often influence the activity of the enzyme through ligand interactions with redox centers, often metals, within the enzyme. Therefore, investigations of the ability of various compounds to inhibit an enzyme's activity, as well as the mechanism of inhibition, can provide insight into the catalytic mechanism of the enzyme as well as the role of various redox centers in catalysis. We have investigated in detail four inhibitors of A. vinelandii and the results are summarized here. The influence of these inhibitors on the spectral properties of the enzyme are summarized. Electron paramagnetic resonance and ultraviolet spectra investigations are discussed. 9 figs.

  18. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control

    SciTech Connect (OSTI)

    Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U. [Univ. of Tennessee, Knoxville, TN (United States); Burlage, R. [Oak Ridge National Lab., TN (United States). Environmental Sciences Div.

    1998-11-01T23:59:59.000Z

    On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

  19. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    solve the energy crisis through biological methods, including genetically engineering algae and cyanobacteria. Create a Sustainable Future: Living Living a Sustainable Future How...

  20. Stringy and Membranic Theory of Swimming of Micro-organisms

    E-Print Network [OSTI]

    Masako Kawamura; Shin'ichi Nojiri; Akio Sugamoto

    1996-03-21T23:59:59.000Z

    When the swimming of micro-organisms is viewed from the string and membrane theories coupled to the velocity field of the fluid, a number of interesting results are derived; 1) importance of the area (or volume) preserving algebra, 2) usefulness of the $N$-point Reggeon (membranic) amplitudes, and of the gas to liquid transition in case of the red tide issues, 3) close relation between the red tide issue and the generation of Einstein gravity, and 4) possible understanding of the three different swimming ways of micro-organisms from the singularity structure of the shape space.

  1. Introduction Microorganisms in sea ice function ecologically in the

    E-Print Network [OSTI]

    Stewart, Frank

    of the pack via brine drainage, ice melting, or ice break-up (Garrison & Buck 1989 and references thereinIntroduction Microorganisms in sea ice function ecologically in the Antarctic marine ecosystem where pelagic and sea ice habitats are intrinsically coupled (Laws 1985, Garrison 1991, Eicken 1992

  2. Early Detection Saves Lives

    Broader source: Energy.gov [DOE]

    Former Department of Energy (DOE) workers tell how medical screening helped them lead healthier and longer lives.

  3. FUTURE LOGISTICS LIVING LABORATORY

    E-Print Network [OSTI]

    Heiser, Gernot

    FUTURE LOGISTICS LIVING LABORATORY Delivering Innovation The Future Logistics Living Lab that will provide logistics solutions for the future. The Living Lab is a demonstration, exhibition and work space by a group of logistics companies, research organisations, universities, and IT providers that includes NICTA

  4. Laboratory and Field Evidence for Long-Term Starvation Survival of Microorganisms in Subsurface Terrestrial Environments

    SciTech Connect (OSTI)

    Kieft, T.L. [Biology Dept., New Mexico Inst. of Mining and Technology, Socorro, NM (United States); Murphy, E.M. [Pacific Northwest National Lab., Richland, WA (United States); Amy, P.S.; Haldeman, D.L. [Department of Biological Sciences, University of Nevada, Las Vegas, NV (United States); Ringelberg, D. B. [Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States)]|[Oak Ridge National Lab., TN (United States)

    1997-12-31T23:59:59.000Z

    BIOGEOCHEMICAL MODELING OF GROUNDWATER FLOW AND NUTRIENT FLUX IN SUBSURFACE ENVIRONMENTS INDICATES THAT INHABITANT MICROORGANISMS EXPERIENCE SEVERE NUTRIENT LIMITATION. USING LABORATORY AND FIELD METHODS, WE HAVE BEEN TESTING STARVATION SURVIVAL IN SUBSURFACE MICROORGANISMS. IN MICROCOSM EXPERIMENTS, WE HAVE SHOWN THAT STRAINS OF TWO COMMONLY ISOLATED SUBSURFACE GENERA, ARTHROBACTER AND PSEUDOMONAS, ARE ABLE TO MAINTAIN VIABILITY IN LOW-NUTRIENT, NATURAL SUBSURFACE SEDIMENTS FOR OVER ONE YEAR. THESE NON-SPORE-FORMING BACTERIA UNDERGO RAPID INITIAL MINIATURIZATION FOLLOWED BY A STABILIZATION OF CELL SIZE. MEMBRANE LIPID PHOSPHOLIPID FATTY ACID (PLFA) PROFILES OF THE PSEUDOMONAS ARE CONSISTENT WITH ADAPTATION TO NUTRIENT STRESS; ARTHROBACTER APPARENTLY RESPONDS TO NUTRIENT DEPRIVATION WITHOUT ALTERING MEMBRANE PLFA. TO TEST SURVIVABILITY OF MICROORGANISMS OVER A GEOLOGIC TIME SCALE, WE CHARACTERIZED MICROBIAL COMMUNITIES IN A SEQUENCE OF UNSATURATED SEDIMENTS RANGING IN AGE FROM MODEM TO {gt}780,000 years. Sediments were relatively uniform silts in Eastern Washington State. Porewater ages at depth (measured by the chloride mass-balance approach) were as old as 3,600 years. Microbial abundance, biomass, and activities (measured by direct counts, culture counts, total PLFAs, and radiorespirometry) declined with sediment age. The pattern is consistent with laboratory microcosm studies of Microbial survival: rapid short-term change followed by long-term survival of a proportion of cells. Even the oldest sediments evinced a small but viable Microbial community. Microbial survival appeared to be a function of sediment age. Porewater age appeared to influence the markup of surviving communities, as indicated by PLFA profiles. Sites with different Porewater recharge rates and patterns of Pleistocene flooding had different communities.

  5. Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons

    DOE Patents [OSTI]

    Fliermans, Carl B. (Augusta, GA)

    1989-01-01T23:59:59.000Z

    A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

  6. Encapsulation method for maintaining biodecontamination activity

    DOE Patents [OSTI]

    Rogers, Robert D.; Hamilton, Melinda A.; Nelson, Lee O.; Benson, Jennifer; Green, Martin J.; Milner, Timothy N.

    2006-04-11T23:59:59.000Z

    A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.

  7. Encapsulation method for maintaining biodecontamination activity

    DOE Patents [OSTI]

    Rogers, Robert D. (Idaho Falls, ID); Hamilton, Melinda A. (Idaho Falls, ID); Nelson, Lee O. (Idaho Falls, ID); Benson, Jennifer (Cockermouth, GB); Green, Martin J. (Wooton, GB); Milner, Timothy N. (Centerville, VA)

    2002-01-01T23:59:59.000Z

    A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.

  8. Effects of remediation amendments on vadose zone microorganisms

    SciTech Connect (OSTI)

    Miller, Hannah M.; Tilton, Fred A.

    2012-08-10T23:59:59.000Z

    Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

  9. Visualizing a Living Building

    E-Print Network [OSTI]

    Padget, Steve

    2013-01-01T23:59:59.000Z

    This paper will chronicle the design process of the Odum School of Ecology at the University of Georgia and its objective to accomplish Living BuildingTM certification. In order to accomplish this, the architect (BNIM) and project partners applied...

  10. Next Generation Living 

    E-Print Network [OSTI]

    Vaughn, Caroline Elizabeth

    2013-02-06T23:59:59.000Z

    of mechanical systems, and our perceived outlook of sustainability can collaborate and aid each other toward sustainable architecture. This collaboration will take form through the proposal or a living and learning community for the students and faculty of Texas...

  11. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNuclear SecurityTechnology |LivingLiving a

  12. Assessing the transport and fate of bioengineered microorganisms in the environment

    SciTech Connect (OSTI)

    Barnthouse, L.W.; Palumbo, A.V.

    1985-01-01T23:59:59.000Z

    We review the methods currently available for quantifying the transport and fate of microbes in atmospheric and aqueous media and assess their adequacy for purposes of risk assessment. We review the literature on transport and fate of microorganisms, including studies of: (1) pathways of migration, (2) the survival of microorganisms during transport and fate. In addition, we review the transport and fate models that have been used in environmental risk assessments for radionuclides and toxic chemicals and evaluate their applicability to the problem of assessing environmental risks of bioengineered microorganisms.

  13. Technical Note: Evaluation of Effective Microorganisms (EM) In Solid Waste Management

    E-Print Network [OSTI]

    Sekeran, V.; Balaji, C.; Bhagavathipushpa, T.

    2005-01-01T23:59:59.000Z

    Microorganisms (EM) In Solid Waste Management V. Sekeran C.is the treatment, disposal and/or recycling of solid wastes.Generally solid waste from a municipality consists of

  14. 2004 Sensory Transduction in Microorganisms Gordon Research Conference-January 11-16, 2004

    SciTech Connect (OSTI)

    Judith Armitage Carlyle Storm

    2005-01-07T23:59:59.000Z

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environment is currently very active in a large number of laboratories in the US, Europe, Japan, and Israel. A wide range of eukaryotic and prokaryotic species are being studies with regard to their sensing of chemical changes, light and redox signal and intercellular signaling, leading either to changes in motile behavior, gene expression or development. It has become increasingly apparent that the mechanisms involved in development have application in higher organisms while the sensing systems in bacteria are involved in a very wide range of physiological traits, from pathogenicity, through to biofilm formation. This is an area where a wide range of state of the art tools have been used and developed over the past few decades. Approaches include behavioral studies, electro-physiology, genetics, molecular biology, structural biology, biophysics and single molecule microscopy, immunocytochemistry and molecular and mathematical modeling, all of this helped by the large number of bacterial and eukaryotic microbial genome sequences now available. The central goal of this meeting is to bring together investigators using this wide range of approaches and different systems to compare data, share ideas and approaches and seeks to understand the fundamental principles underlying these responses.

  15. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14T23:59:59.000Z

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  16. Anaerobic Redox Cycling of Iron by Freshwater Sediment Microorganisms

    SciTech Connect (OSTI)

    Weber, Karrie A.; Urrutia, Matilde M.; Churchill, Perry F.; Kukkadapu, Ravi K.; Roden, Eric E.

    2006-01-01T23:59:59.000Z

    The potential for microbially-mediated anaerobic redox cycling of iron (Fe) was examined in a first-generation enrichment culture of freshwater wetland sediment microorganisms. MPN enumerations revealed the presence of significant populations of Fe(III)-reducing (ca. 108 cells mL-1) and Fe(II)-oxidizing, nitrate-reducing organisms (ca. 105 cells mL-1) in the sediment used to inoculate the enrichment cultures. Nitrate reduction commenced immediately following inoculation of acetate-containing (ca. 1 mM) medium with a small quantity (1% vol/vol) of wetland sediment, and resulted in the transient accumulation of NO2- and production of a mixture of end-products including NH4+. Fe(III) oxide (high surface area goethite) reduction took place - after NO3- was depleted and continued until all the acetate was utilized. Addition of NO3 after Fe(III) reduction ceased resulted in the immediate oxidation of Fe(II) coupled to reduction of + NO3-to NH4 . No significant NO2- accumulation was observed during nitrate-dependent Fe(II) oxidation. No Fe(II) oxidation occurred in pasteurized controls. Microbial community structure in the enrichment was monitored by DGGE analysis of PCR amplified 16s rDNA and RT-PCR amplified 16S rRNA, as well as by construction of 16S rDNA clone libraries for four different time points during the experiment. Strong similarities in dominant members of the microbial community were observed in the Fe(III) reduction and nitrate-dependent Fe(II) oxidation phases of the experiment, specifically the common presence of organisms closely related (= 95% sequence similarity) to the genera Geobacter and Dechloromonas. These results indicate that the wetland sediments contained organisms such as Geobacter sp. which are capable of both + dissimilatory Fe(III) reduction and oxidation of Fe(II) with reduction of NO3-reduction to NH4 . Our findings suggest that microbially-catalyzed nitrate-dependent Fe(II) oxidation has the potential to contribute to a dynamic anaerobic Fe redox cycle in freshwater sediments.

  17. Oil Production by a Consortium of Oleaginous Microorganisms grown on primary effluent wastewater

    SciTech Connect (OSTI)

    Hall, Jacqueline; Hetrick, Mary; French, Todd; Hernandez, Rafael; Donaldson, Janet; Mondala, Andro; Holmes, William

    2011-01-01T23:59:59.000Z

    Municipal wastewater could be a potential growth medium that has not been considered for cultivating oleaginous microorganisms. This study is designed to determine if a consortium of oleaginous microorganism can successfully compete for carbon and other nutrients with the indigenous microorganisms contained in primary effluent wastewater. RESULTS: The oleaginous consortium inoculated with indigenous microorganisms reached stationary phase within 24 h, reaching a maximum cell concentration of 0.58 g L -1. Water quality post-oleaginous consortium growth reached a maximum chemical oxygen demand (COD) reduction of approximately 81%, supporting the consumption of the glucose within 8 h. The oleaginous consortium increased the amount of oil produced per gram by 13% compared with indigenous microorganisms in raw wastewater. Quantitative polymerase chain reaction (qPCR) results show a substantial population increase in bacteria within the first 24 h when the consortium is inoculated into raw wastewater. This result, along with the fatty acid methyl esters (FAMEs) results, suggests that conditions tested were not sufficient for the oleaginous consortium to compete with the indigenous microorganisms.

  18. Recovery of uranium by using new microorganisms isolated from North American uranium deposits

    SciTech Connect (OSTI)

    Sakaguchi, T.; Nakajima, A.; Tsuruta, T. [Miyazaki Medical College (Japan)

    1995-12-31T23:59:59.000Z

    Some attempts were made to remove uranium that may be present in refining effluents, mine tailings by using new microorganisms isolated from uranium deposits and peculiar natural environments. To screen microorganisms isolated from uranium deposits and peculiar natural environments in North America and Japan for maximal accumulation of uranium, hundreds of microorganisms were examined. Some microorganisms can accumulate about 500 mg (4.2 mEq) of uranium per gram of Microbial cells within 1 h. The uranium accumulation capacity of the cells exceeds that of commercially available chelating agents (2-3 mEq/g adsorbent). We attempted to recover uranium from uranium refining waste water by using new microorganisms. As a result, these microbial cells can recover trace amounts of uranium from uranium waste water with high efficiency. These strains also have a high accumulating ability for thorium. Thus, these new microorganisms can be used as an adsorbing agent for the removal of nuclear elements may be present in metallurgical effluents, mine tailings and other waste sources.

  19. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, Richard R.; Bauman, Robert

    2006-11-14T23:59:59.000Z

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  20. Living SafeLy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNuclear SecurityTechnology |Living SafeLy

  1. Living With Wildfire

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOEThe Bonneville PowerCherries 82981-1cnHigh SchoolIn12electron 9 5Let usNuclear SecurityTechnology |Living

  2. Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport

    SciTech Connect (OSTI)

    Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

    2013-09-28T23:59:59.000Z

    Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

  3. Representation of Dormant and Active Microbial Dynamics for Ecosystem Modeling

    SciTech Connect (OSTI)

    Wang, Gangsheng [ORNL; Mayes, Melanie [ORNL; Gu, Lianhong [ORNL; Schadt, Christopher Warren [ORNL

    2014-01-01T23:59:59.000Z

    Dormancy is an essential strategy for microorganisms to cope with environmental stress. However, global ecosystem models typically ignore microbial dormancy, resulting in notable model uncertainties. To facilitate the consideration of dormancy in these large-scale models, we propose a new microbial physiology component that works for a wide range of substrate availabilities. This new model is based on microbial physiological states and the major parameters are the maximum specific growth and maintenance rates of active microbes and the ratio of dormant to active maintenance rates. A major improvement of our model over extant models is that it can explain the low active microbial fractions commonly observed in undisturbed soils. Our new model shows that the exponentially-increasing respiration from substrate-induced respiration experiments can only be used to determine the maximum specific growth rate and initial active microbial biomass, while the respiration data representing both exponentially-increasing and non-exponentially-increasing phases can robustly determine a range of key parameters including the initial total live biomass, initial active fraction, the maximum specific growth and maintenance rates, and the half-saturation constant. Our new model can be incorporated into existing ecosystem models to account for dormancy in microbially-driven processes and to provide improved estimates of microbial activities.

  4. A BUCKEYE'S GUIDE TO LIVING OFF-CAMPUS LIVING GUIDE

    E-Print Network [OSTI]

    Howat, Ian M.

    2014­2015 FREE A BUCKEYE'S GUIDE TO LIVING OFF-CAMPUS LIVING GUIDE OFFICE OF STUDENT LIFE@osu.edu offcampus.osu.edu WELCOME TO OFF-CAMPUS LIVING! 3 #12;TABLE OF CONTENTS OFFICE OF STUDENT LIFE 9 62 Stay Healthy 66 The Basics 67 Get Involved 68 Moving Out 5 #12;1 Bedrooms: 2262 N. H IG H 49 E. N

  5. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z [Northeastern University; Wan, Kai-tak [Northeastern Univeristy

    2014-09-02T23:59:59.000Z

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  6. activated sludge micro-organism: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of each treatment process, according to the Egyptian conditions, is evaluated. The Life cycle assessment methodology was applied to assess and compare the environmental...

  7. The living publication

    SciTech Connect (OSTI)

    Terwilliger, Thomas C. [Los Alamos National Laboratory

    2012-06-04T23:59:59.000Z

    Within the ICSTI Insights Series we offer three articles on the 'living publication' that is already available to practitioners in the important field of crystal structure determination and analysis. While the specific examples are drawn from this particular field, we invite readers to draw parallels in their own fields of interest. The first article describes the present state of the crystallographic living publication, already recognized by an ALPSP (Association of Learned and Professional Society Publishers) Award for Publishing Innovation in 2006. The second article describes the potential impact on the record of science as greater post-publication analysis becomes more common within currently accepted data deposition practices, using processed diffraction data as the starting point. The third article outlines a vision for the further improvement of crystallographic structure reports within potentially achievable enhanced data deposition practices, based upon raw (unprocessed) diffraction data. The IUCr in its Commissions and Journals has for many years emphasized the importance of publications being accompanied by data and the interpretation of the data in terms of atomic models. This has been followed as policy by numerous other journals in the field and its cognate disciplines. This practice has been well served by databases and archiving institutions such as the Protein Data Bank (PDB), the Cambridge Crystallographic Data Centre (CCDC), and the Inorganic Crystal Structure Database (ICSD). Normally the models that are archived are interpretations of the data, consisting of atomic coordinates with their displacement parameters, along with processed diffraction data from X-ray, neutron or electron diffraction studies. In our current online age, a reader can not only consult the printed word, but can display and explore the results with molecular graphics software of exceptional quality. Furthermore, the routine availability of processed diffraction data allows readers to perform direct calculations of the electron density (using X-rays and electrons as probes) or the nuclear density (using neutrons as probe) on which the molecular models are directly based. This current community practice is described in our first article. There are various ways that these data and tools can be used to further analyze the molecules that have been crystallized. Notably, once a set of results is announced via the publication, the research community can start to interact directly with the data and models. This gives the community the opportunity not only to read about the structure, but to examine it in detail, and even generate subsequent improved models. These improved models could, in principle, be archived along with the original interpretation of the data and can represent a continuously improving set of interpretations of a set of diffraction data. The models could improve both by correction of errors in the original interpretation and by the use of new representations of molecules in crystal structures that more accurately represent the contents of a crystal. These possible developments are described in our second article. A current, significant, thrust for the IUCr is whether it would be advantageous for the crystallographic community to require, rather than only encourage, the archiving of the raw (unprocessed) diffraction data images measured from a crystal, a fibre or a solution. This issue is being evaluated in detail by an IUCr Working Group (see http://forums.iucr.org). Such archived raw data would be linked to and from any associated publications. The archiving of raw diffraction data could allow as yet undeveloped processing methods to have access to the originally measured data. The debate within the community about this much larger proposed archiving effort revolves around the issue of 'cost versus benefit'. Costs can be minimized by preserving the raw data in local repositories, either at centralized synchrotron and neutron research institutes, or at research universities. Archiving raw data is also perceived as bein

  8. What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under

    E-Print Network [OSTI]

    Mukhtar, Saqib

    What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under aerobic conditions (in the presence of oxygen). During the composting is production of good-quality compost that is biologically stable, relatively uniform in appearance, free

  9. The Living Culture of Nigeria

    E-Print Network [OSTI]

    Biobaku, Saburi O.; Aniakor, Chike A.

    1977-01-01T23:59:59.000Z

    ImK ~IE.W THE uv:rn:; aJLWRE OF NIGERIA edited by Saburi 0 .Lagos: Thanas Nelson (Nigeria) Ltd. , Color Illustrations,86 The Living Cu'lture of Nigeria edited by Professor Saburi

  10. Blood Banking in Living Droplets

    E-Print Network [OSTI]

    Samot, Josh

    Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional ...

  11. Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production

    SciTech Connect (OSTI)

    None

    2010-07-15T23:59:59.000Z

    Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

  12. Effects of microorganisms growth on the long-term stability of cement and bitumen

    SciTech Connect (OSTI)

    Libert, M.F.; Sellier, R.; Jouquet, G.; Trescinski, M.; Spor, H. [Nuclear Research Center of Cadarache, St.Paul-Lez-Durance (France)

    1993-12-31T23:59:59.000Z

    Cement is used as a coating matrix for nuclear waste or as an engineered barrier of waste repositories situated in geological formations. The effect of mineral acids excreted by bacteria (Thiobacillus) or organic acids produced by fungi, on the biodegradation of cement is discussed. Organic acids are quantitatively and qualitatively determined during growth of fungi over a two-year period. Even with high pH conditions, pH of the cement {approx} 11, growth of microorganisms occurs. Biodeterioration of cement is expressed in terms of bioleaching velocity of calcium and is observed by electron microscopy. Bitumen is commonly used as a matrix for the long-term storage of radioactive wastes. Long-term biodegrability of bitumen is discussed as a function of its chemical composition and various studied microorganisms.

  13. Subsurface interactions of actinide species and microorganisms : implications for the bioremediation of actinide-organic mixtures.

    SciTech Connect (OSTI)

    Banaszak, J.E.; Reed, D.T.; Rittmann, B.E.

    1999-02-12T23:59:59.000Z

    By reviewing how microorganisms interact with actinides in subsurface environments, we assess how bioremediation controls the fate of actinides. Actinides often are co-contaminants with strong organic chelators, chlorinated solvents, and fuel hydrocarbons. Bioremediation can immobilize the actinides, biodegrade the co-contaminants, or both. Actinides at the IV oxidation state are the least soluble, and microorganisms accelerate precipitation by altering the actinide's oxidation state or its speciation. We describe how microorganisms directly oxidize or reduce actinides and how microbiological reactions that biodegrade strong organic chelators, alter the pH, and consume or produce precipitating anions strongly affect actinide speciation and, therefore, mobility. We explain why inhibition caused by chemical or radiolytic toxicities uniquely affects microbial reactions. Due to the complex interactions of the microbiological and chemical phenomena, mathematical modeling is an essential tool for research on and application of bioremediation involving co-contamination with actinides. We describe the development of mathematical models that link microbiological and geochemical reactions. Throughout, we identify the key research needs.

  14. arXiv:0812.2887v1[cond-mat.soft]15Dec2008 The hydrodynamics of swimming microorganisms

    E-Print Network [OSTI]

    Podgornik, Rudolf

    microorganisms move are familiar. Bacteria such as Escherichia coli detect gradients in nutrients and move, and the marine life ecosystem. Here we review the biophysical and mechanical principles of locomotion

  15. Effects of selected thermophilic microorganisms on crude oils at elevated temperatures and pressures. Final report

    SciTech Connect (OSTI)

    Premuzic, E.T.; Lin, M.S.

    1995-07-01T23:59:59.000Z

    During the past several years, a considerable amount of work has been carried out showing that microbially enhanced oil recovery (MEOR) is promising and the resulting biotechnology may be deliverable. At the Brookhaven National Laboratory (BNL), systematic studies have been conducted which dealt with the effects of thermophilic and thermoadapted bacteria on the chemical and physical properties of selected types of crude oils at elevated temperatures and pressures. Particular attention was paid to heavy crude oils from Venezuela, California, Alabama, Arkansas, Wyoming, Alaska, and other oil producing areas. Current studies indicate that during the biotreatment several chemical and physical properties of crude oils are affected. The oils are (1) emulsified; (2) acidified; (3) there is a qualitative and quantitative change in light and heavy fractions of the crudes; (4) there are chemical changes in fractions containing sulfur compounds; (5) there is an apparent reduction in the concentration of trace metals; (6) the qualitative and quantitative changes appear to be microbial species dependent; and (7) there is a distinction between {open_quotes}biodegraded{close_quotes} and {open_quotes}biotreated{close_quotes} oils. Preliminary results indicate the introduced microorganisms may become the dominant species in the bioconversion of oils. These studies also indicate the biochemical interactions between crude oils and microorganisms follow distinct trends, characterized by a group of chemical markers. Core-flooding experiments have shown significant additional crude oil recoveries are achievable with thermophilic microorganisms at elevated temperatures similar to those found in oil reservoirs. In addition, the biochemical treatment of crude oils has technological applications in downstream processing of crude oils such as in upgrading of low grade oils and the production of hydrocarbon based detergents.

  16. THE IMPACT OF MICROBIAL CHELATES ON MINERAL WEATHERING AND MICROBIAL METABOLIC ACTIVITY

    E-Print Network [OSTI]

    Kulczycki, Ezra

    2010-04-25T23:59:59.000Z

    composition and cellular design. A relationship between geologic host materials and biological activity is purported. The role of microorganisms upon mineral weathering was investigated by collecting soil samples from the tropical rainforest of Barro Colorado...

  17. A comparison of taxon co-occurrence patterns for macro- and microorganisms

    E-Print Network [OSTI]

    Horner-Devine, M. Claire; Silver, Jessica M.; Leibold, Mathew A.; Bohannan, Brendan J. M.; Colwell, Robert K.; Fuhrman, Jed A.; Green, Jessica L.; Kuske, Cheryl R.; Martiny, Jennifer B. H.; Muyzer, Gerard; Ø vreå s, Lise; Reysenbach, Anna-Louise; Smith, Val H.

    2007-06-01T23:59:59.000Z

    . Holmes, M. Westoby, I. Oliver, D. Briscoe, M. Dangerfield, M. Gillings, and A. Beattie. 2004. Spatial scaling of microbial eukaryote diversity. Nature 432:747–750. Hillebrand, H., F. Watermann, R. Karez, and U. G. Berninger. 2001. Differences in species...Ecology, 88(6), 2007, pp. 1345–1353 #1; 2007 by the Ecological Society of America A COMPARISON OF TAXON CO-OCCURRENCE PATTERNS FOR MACRO- AND MICROORGANISMS M. CLAIRE HORNER-DEVINE,1,13 JESSICA M. SILVER,1 MATHEW A. LEIBOLD,2 BRENDAN J. M. BOHANNAN...

  18. From Where You Live to Where You Spend Time: Environmental Contributions to Obesity Risk

    E-Print Network [OSTI]

    Illinois at Chicago, University of

    From Where You Live to Where You Spend Time: Environmental Contributions to Obesity Risk Shannon N contributions to obesity risk Where you live Where you spend time Activity spaces EMA #12;I will mostly can choose from, and we should have that same thing. -Chicago resident #12;Environment and Obesity

  19. Meso-scale turbulence in living fluids

    E-Print Network [OSTI]

    Henricus H. Wensink; Jörn Dunkel; Sebastian Heidenreich; Knut Drescher; Raymond E. Goldstein; Hartmut Löwen; Julia M. Yeomans

    2012-08-21T23:59:59.000Z

    Turbulence is ubiquitous, from oceanic currents to small-scale biological and quantum systems. Self-sustained turbulent motion in microbial suspensions presents an intriguing example of collective dynamical behavior amongst the simplest forms of life, and is important for fluid mixing and molecular transport on the microscale. The mathematical characterization of turbulence phenomena in active non-equilibrium fluids proves even more difficult than for conventional liquids or gases. It is not known which features of turbulent phases in living matter are universal or system-specific, or which generalizations of the Navier-Stokes equations are able to describe them adequately. Here, we combine experiments, particle simulations, and continuum theory to identify the statistical properties of self-sustained meso-scale turbulence in active systems. To study how dimensionality and boundary conditions affect collective bacterial dynamics, we measured energy spectra and structure functions in dense Bacillus subtilis suspensions in quasi-2D and 3D geometries. Our experimental results for the bacterial flow statistics agree well with predictions from a minimal model for self-propelled rods, suggesting that at high concentrations the collective motion of the bacteria is dominated by short-range interactions. To provide a basis for future theoretical studies, we propose a minimal continuum model for incompressible bacterial flow. A detailed numerical analysis of the 2D case shows that this theory can reproduce many of the experimentally observed features of self-sustained active turbulence.

  20. Solo living across the adult lifecourse 

    E-Print Network [OSTI]

    Smith, Adam; Wasoff, Fran; Jamieson, Lynn

    This study has created snapshots of solo living in contemporary society and developed an understanding of the social and economic factors involved in transitions in and out of solo living.

  1. Detection and quantification of waterborne microorganisms using an image cytometer based on angular spatial frequency processing

    E-Print Network [OSTI]

    Pérez, Juan Miguel; Martínez, Pedro; Pruneri, Valerio

    2015-01-01T23:59:59.000Z

    We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off--the--shelf components, such as a light emitting diode (LED) source, a complementary metal--oxide--semiconductor (CMOS) image sensor, and a specific combination of optical lenses that allow, through an appropriate software, Fourier transform processing of the sample volume. Waterborne microorganisms, such as Escherichia coli (E. coli), Legionella pneumophila (L. pneumophila) and Phytoplankton, are detected by interrogating the volume sample either in a fluorescent or label-free mode, i.e. with or without fluorescein isothiocyanate (FITC) molecules attached to the micro-organisms, respectively. We achieve a sensitivity of 50 CFU/ml, which can be further increased to 0.2 CFU/ml by pre-concentrating an initial sample volume of 500 ml with an ad hoc fluidic system. We also prove the capability of the proposed image cytometer of diffe...

  2. Living in Germany. Studying at FAU.

    E-Print Network [OSTI]

    Fiebig, Peter

    Welcome. Living in Germany. Studying at FAU. www.fau.eu/international #12;Welcome. Living in Germany. Studying at FAU. 2 Welcome. Living in Germany. Studying at FAU. Contents Vice President`s welcome...................................................... 5 1. What you will find in this brochure .............................. 6 2. Welcome to Germany

  3. Live Streaming with Gossip Maxime Monod

    E-Print Network [OSTI]

    Guerraoui, Rachid

    Live Streaming with Gossip Maxime Monod June 30, 2010 #12;Regular TV: everything HD Live streaming A source produces multimedia content n viewers (n large) broadcasting ... ... ... IP TV, Web TV, P2P TV environment ·HEAP Heterogeneous environment ·LiFT Presence of freeriders Live Streaming with Gossip 25 #12

  4. Quantitative phase microscopy for the study of electromotility in living cells

    E-Print Network [OSTI]

    Oh, Seung-eun

    2010-01-01T23:59:59.000Z

    The electric activity of living cells is accompanied with changes in their optical and mechanical properties, which arise from the intrinsic biophysics of the cell membrane. These intrinsic changes can be used as an indicator ...

  5. Transcription Inhibition by Platinum DNA Cross-links in Live Mammalian Cells

    E-Print Network [OSTI]

    Ang, Wee Han

    We have investigated the processing of site-specific Pt?DNA cross-links in live mammalian cells to enhance our understanding of the mechanism of action of platinum-based anticancer drugs. The activity of platinum drugs ...

  6. SF State | Residential Life Community Living Standards Page 1 COMMUNITY LIVING STANDARDS

    E-Print Network [OSTI]

    SF State | Residential Life Community Living Standards Page 1 COMMUNITY LIVING STANDARDS A How-To Guide to Living in the SF State Residential Community 2014-2015 #12;SF State | Residential Life Community Philosophy Residential Life Staff Campus Resources One Stop Your Roommate Your Living Space

  7. adolescent population living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that adolescents assign (more) Amos, Taryn 2009-01-01 94 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  8. actual living liver: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    - Liver EQA slides' 2. Electronic submissions Magee, Derek 67 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  9. abortus live vaccine: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    healthcare needs. TheVirginia Tech Virginia Tech 183 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  10. african americans living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of human African trypanosomiasis (HAT Schnaufer, Achim 375 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  11. american indians living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    positive correlations with the Indian Chang, Chih-Pei 262 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  12. advanced cancer living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    direct and (more) Duberow, Jr, David Paul 2009-01-01 100 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  13. attenuated live vaccine: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    healthcare needs. TheVirginia Tech Virginia Tech 211 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  14. adult living donor: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    to extending our successes in childhood unknown authors 116 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  15. american women living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    introduces teachers to Maya culture from Saldin, Dilano 295 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  16. admixed population lived: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    population in urban United Nations; Nations Unies 1999-01-01 67 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  17. acomys cahirinus living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 18 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  18. attenuated live infectious: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    R. Matyas; Victoria R. Polonis; Mangala Rao; Carl R. Alving 169 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  19. analysis reveals living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Oleg V. Gradov; Margaret A. Gradova 2015-01-18 184 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  20. arab americans living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Syria. The three countries show different Shavitt, Yuval 380 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  1. adolescent girls living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    that adolescents assign (more) Amos, Taryn 2009-01-01 139 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  2. african women living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and Siegal 1991). Marvin P. Dawkins; Mary M. Williams 273 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  3. asthmatic children living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Texas; Kevin Purcell; Jaime Fergie; Kevin Richman; Lisa Rocha 84 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  4. Aerobic mineralization of MTBE and tert-butyl alcohol by stream-bed sediment microorganisms

    SciTech Connect (OSTI)

    Bradley, P.M.; Landmeyer, J.E.; Chapelle, F.H. [Geological Survey, Columbia, SC (United States)] [Geological Survey, Columbia, SC (United States)

    1999-06-01T23:59:59.000Z

    Microorganisms indigenous to the stream-bed sediments at two gasoline-contaminated groundwater sites demonstrated significant mineralization of the fuel oxygenates, methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA). Up to 73% of [U-{sup 14}C]-MTBE and 84% of [U-{sup 14}C]-TBA were degraded to {sup 14}CO{sub 2} under mixed aerobic/anaerobic conditions. No significant mineralization was observed under strictly anaerobic conditions. The results indicate that, under the mixed aerobic/anaerobic conditions characteristic of stream-bed sediments, microbial processes may provide a significant environmental sink for MTBE and TBA delivered to surface water bodies by contaminated groundwater or by other sources.

  5. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02T23:59:59.000Z

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  6. How sulphate-reducing microorganisms cope with stress: Lessons from systems biology

    SciTech Connect (OSTI)

    Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

    2011-04-01T23:59:59.000Z

    Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

  7. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

    1996-02-20T23:59:59.000Z

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

  8. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.

    1996-02-20T23:59:59.000Z

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

  9. Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium

    SciTech Connect (OSTI)

    Sadler, Natalie C.; Melnicki, Matthew R.; Serres, Margrethe H.; Merkley, Eric D.; Chrisler, William B.; Hill, Eric A.; Romine, Margaret F.; Kim, Sangtae; Zink, Erika M.; Datta, Suchitra; Smith, Richard D.; Beliaev, Alex S.; Konopka, Allan; Wright, Aaron T.

    2014-01-01T23:59:59.000Z

    Protein reduction-oxidation (redox) modification is an important mechanism that allows microorganisms to sense environmental changes and initiate cellular responses. We have developed a quantitative chemical probe approach for live cell labeling of proteins that are sensitive to redox modifications. We utilize this in vivo strategy to identify 176 proteins undergoing ~5-10 fold dynamic redox change in response to nutrient limitation and subsequent replenishment in the photoautotrophic cyanobacterium, Synechococcus sp. PCC 7002. We detect redox changes in as little as 30 seconds after nutrient perturbation, and oscillations in reduction and oxidation for 60 minutes following the perturbation. Many of the proteins undergoing dynamic redox transformations participate in the major components for the production (photosystems and electron transport chains) or consumption (Calvin-Benson cycle and protein synthesis) of reductant and/or energy in photosynthetic organisms. Thus, our in vivo approach reveals new redox-susceptible proteins, in addition to validating those previously identified in vitro.

  10. Sustainable Living Guide This sustainable living guide is the product of a class project for Geography 6250 at Memorial

    E-Print Network [OSTI]

    Oyet, Alwell

    St. John's Sustainable Living Guide #12;This sustainable living guide is the product of a class's Sustainable Living Guide. Memorial University of Newfoundland, St. John's, Newfoundland, Canada, 40 p. Any...........................................................................................................2-4 Sustainable Home Living

  11. Live with the Energy Innovation Hub Directors

    Broader source: Energy.gov [DOE]

    Tune in today, Tuesday, March 6, 2012, at 2:15pm EST for a live discussion with our three Energy Innovation Hubs directors.

  12. Nuclear Physics Technology Saves Lives | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Saves Lives January 11, 2006 Listen to this story Ribbon With early detection, breast cancer can often be treated successfully. There are over two million breast cancer survivors...

  13. Extending the Operating Lives of Materials

    ScienceCinema (OSTI)

    Paul Jablonski

    2010-09-01T23:59:59.000Z

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  14. Extending the Operating Lives of Materials

    SciTech Connect (OSTI)

    Paul Jablonski

    2010-01-26T23:59:59.000Z

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  15. Evaluation of terrestrial microcosms for assessing the fate and effects of genetically engineered microorganisms on ecological processes

    SciTech Connect (OSTI)

    Fredrickson, J.K.; Bentjen, S.A.; Bolton, H. Jr.; Li, S.W.; Ligotke, M.W.; McFadden, K.M.; Van Voris, P.

    1989-04-01T23:59:59.000Z

    This project evaluates and modifies the existing US Environmental Protection Agency's Office of Pesticides and Toxic Substances (EPA/OPTS) terrestrial microcosm test system and test protocols such that they can be used to determine the environmental fate and ecological hazards of genetically engineered microorganisms (GEMs). The intact soil-core microcosm represents terrestrial ecosystems, and when coupled with appropriate test protocols, such microcosms may be appropriate to define and limit risks associated with the intentional release of GEMs. The terrestrial microcosm test system was used to investigate the survival and transport of two model GEMs (Azospirillum lipoferum and Pseudomonas sp. Tn5 mutants) to various trophic levels and niches and through intact soil cores. Subsequent effects on nutrient cycling and displacement of indigenous microorganisms were evaluated. The model organisms were a diazotrophic root-colonizing bacterium (A. lipoferum) and a wheat root growth-inhibiting rhizobacterium (Pseudomonas sp.). The transposable element Tn5 was used as a genetic marker for both microorganisms in two separate experiments. The organisms were subjected to transposon mutagenesis using a broad host-range-mobilizable suicide plasmid. The transposon Tn5 conferred levels of kanamycin resistance up to 500 ..mu..g/ml (Pseudomonas sp.), which allowed for selection of the bacteria from environmental samples. The presence of Tn5 DNA in the genome of the model GEMs also allowed the use of Tn5 gene probes to confirm and enumerate the microorganisms in different samples from the microcosms. Two types of root growth-inhibiting Pseudomonas sp. Tn5 mutants were obtained and used in microcosm studies: those that lacked the ability to inhibit either wheat root growth or the growth of other microorganisms in vitro (tox/sup /minus//) and those which retained these properties (tox/sup +/). 53 refs., 7 figs., 6 tabs.

  16. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07T23:59:59.000Z

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  17. s Earth and environment s Living resources

    E-Print Network [OSTI]

    4 s Earth and environment s Living resources s Societies and health s Expertise and consulting of this trend is the acquisition, on a joint proposal from the Earth and Environment department and the Living phenomena so as to improve forecasting of the attendant hazards. The earth's crust: processes and natural

  18. INVEST IN YOUR BONES Living with Osteoporosis

    E-Print Network [OSTI]

    INVEST IN YOUR BONES Living with Osteoporosis Leaflet 5 Living with osteoporosis can be done environment safe to avoid falls. Early detection of bone loss or osteoporosis is now possible with bone to be most effective in reducing bone loss during the five to ten years following menopause, when bone loss

  19. Living Village Kioni Village, Ithaca, Greece

    E-Print Network [OSTI]

    Zaferatos, Nicholas C.

    University | 2010 #12;Living | Village Kioni Village Ithaca, Greece A X A A Sustainable Ithaca ProgramLiving Village Kioni Village, Ithaca, Greece A X A A Nicholas Zaferatos Western Washington Zaferatos, Sustainable Ithaca Faculty Program Director, at nicholas.zaferatos@wwu.edu #12;Table of Contents

  20. 1 | P a g e Living a Digital Life Camp Camp: Living a Digital Life

    E-Print Network [OSTI]

    Seldin, Jonathan P.

    , captchas, social media, MMORPG -- this camp explores the digital world from both the user1 | P a g e Living a Digital Life Camp Camp: Living a Digital Life August 11- 15 for participants 11 - 13 years old General description: We live in a digital world, where the ground shifts faster

  1. High-Sensitivity Delayed-Coincidence Spectrometer to Search for Short-Lived Nuclear States

    E-Print Network [OSTI]

    Morozov, V A; Norseev, Yu V; Sereeter, Z; Zlokazov, V B

    2002-01-01T23:59:59.000Z

    A high-sensitivity four-dimensional triple-coincidence spectrometer with two detectors is developed on the basis of an autocorrelation single-crystal scintillation time 4{\\pi} spectrometer and a semiconductor HPGe detector. The delayed-coincidence spectrometer is designed for searching for isomeric nano- and microsecond states in short-lived nuclei and for their related gamma-rays. Half-lives of short-lived states are measured in the range from 4 ns to 4 {\\mu}s. The sufficient activity of the monoisotopic source for the investigations does not exceed 0.01 {\\mu}Ci.

  2. An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

    SciTech Connect (OSTI)

    Holmes, Aimee E.; Sego, Landon H.; Webb-Robertson, Bobbie-Jo M.; Kreuzer, Helen W.; Anderson, Richard M.; Unwin, Stephen D.; Weimar, Mark R.; Tardiff, Mark F.; Corley, Courtney D.

    2013-02-01T23:59:59.000Z

    We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

  3. Metallomics of two microorganisms relevant to heavy metal bioremediation reveal fundamental differences in metal assimilation and utilization

    SciTech Connect (OSTI)

    Lancaster, Andrew [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Menon, Angeli [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Scott, Israel [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Poole, Farris [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Vaccaro, Brian [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Thorgersen, Michael P [ORNL] [ORNL; Geller, Jil [Lawrence Berkeley National Laboratory (LBNL)] [Lawrence Berkeley National Laboratory (LBNL); Hazen, Terry C [ORNL] [ORNL; Hurt, Jr., Richard Ashley [ORNL; Brown, Steven D [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Adams, Michael W. W. [University of Georgia, Athens, GA] [University of Georgia, Athens, GA

    2014-01-01T23:59:59.000Z

    Although as many as half of all proteins are thought to require a metal cofactor, the metalloproteomes of microorganisms remain relatively unexplored. Microorganisms from different environments are likely to vary greatly in the metals that they assimilate, not just among the metals with well-characterized roles but also those lacking any known function. Herein we investigated the metal utilization of two microorganisms that were isolated from very similar environments and are of interest because of potential roles in the immobilization of heavy metals, such as uranium and chromium. The metals assimilated and their concentrations in the cytoplasm of Desulfovibrio vulgaris strain Hildenborough (DvH) and Enterobacter cloacae strain Hanford (EcH) varied dramatically, with a larger number of metals present in Enterobacter. For example, a total of 9 and 19 metals were assimilated into their cytoplasmic fractions, respectively, and DvH did not assimilate significant amounts of zinc or copper whereas EcH assimilated both. However, bioinformatic analysis of their genome sequences revealed a comparable number of predicted metalloproteins, 813 in DvH and 953 in EcH. These allowed some rationalization of the types of metal assimilated in some cases (Fe, Cu, Mo, W, V) but not in others (Zn, Nd, Ce, Pr, Dy, Hf and Th). It was also shown that U binds an unknown soluble protein in EcH but this incorporation was the result of extracellular U binding to cytoplasmic components after cell lysis.

  4. Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report

    SciTech Connect (OSTI)

    Elmore, B.B.

    1993-08-01T23:59:59.000Z

    As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

  5. campus as living laboratory institutional Sustainability Plan

    E-Print Network [OSTI]

    Calgary, University of

    operations campus as living laboratory diversity campus waste institutional Sustainability Plan eliminate priorities energy research 35%ofwastedivertedSTARSSilver 275 sustainability courses investment 60 accessibility faculty leadership 100+ sustainability research projects over $3.8 million in bursaries Calgary

  6. Adapting Your Home for More Accessible Living

    E-Print Network [OSTI]

    Harris, Janie

    2007-04-27T23:59:59.000Z

    It is important for people to live comfortably and independently in their homes. Homes can be adapted to aid people with various disabilities. This publication explains how to make such adaptations for people with vision loss, hearing loss, problems...

  7. Living With Roommates a practical guide

    E-Print Network [OSTI]

    Loudon, Catherine

    /Cleaning (personal living area) y Mail pick-up y Pet peeves y Smoking y Food (sharing/cooking) y Room temperature y Decorations y Alcohol (consumption values & practices) y Telephone/payment/usage/messages Suggested Topics

  8. The Live Room : transducing resonant architectures

    E-Print Network [OSTI]

    Bain, Mark, 1966-

    1998-01-01T23:59:59.000Z

    The Live Room is a temporary site specific installation presented in building N 51, room 117 on the MIT campus on May 7, 1998 and concluded on June 10, 1998. Using small acoustic intensifying equipment which mount directly ...

  9. Ultrahigh-Resolution Magnetic Resonance in Inhomogeneous Magnetic Fields: Two-Dimensional Long-Lived-Coherence Correlation Spectroscopy

    E-Print Network [OSTI]

    sophisticated gradient switching or frequency-modulated pulses. Applications to in-cell NMR and to magnetic, metabolism, blood flow, diffusion, and brain activity. Most applications to inanimate samples and living

  10. Activity Recognition for Natural Human Robot Interaction

    E-Print Network [OSTI]

    Ravindran, Balaraman

    Activity Recognition for Natural Human Robot Interaction Addwiteey Chrungoo1 , SS Manimaran between humans and robots. While humans can distinguish between communicative actions and activities of daily living, robots cannot draw such inferences effectively. To allow intuitive human robot interaction

  11. Dynamical real numbers and living systems

    E-Print Network [OSTI]

    Dhurjati Prasad Datta

    2010-01-11T23:59:59.000Z

    Recently uncovered second derivative discontinuous solutions of the simplest linear ordinary differential equation define not only an nonstandard extension of the framework of the ordinary calculus, but also provide a dynamical representation of the ordinary real number system. Every real number can be visualized as a living cell -like structure, endowed with a definite evolutionary arrow. We discuss the relevance of this extended calculus in the study of living systems. We also present an intelligent version of the Newton's first law of motion.

  12. The hydrodynamics of swimming microorganisms This article has been downloaded from IOPscience. Please scroll down to see the full text article.

    E-Print Network [OSTI]

    Lauga, Eric

    . The reasons microorganisms move are familiar. Bacteria such as Escherichia coli detect gradients in nutrients processes, including reproduction, infection and the marine life ecosystem. Here we review the biophysical

  13. In Situ Live Cell Sensing of Multiple Nucleotides Exploiting...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNARNA Aptamers and Graphene Oxide Nanosheets. In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNARNA...

  14. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Environmental Management (EM)

    Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and...

  15. Live Webinar on Better Buildings Challenge: Public-Sector Update

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "Better Buildings Challenge: Public-Sector Update."

  16. housing.ucdavis.edu Living at UC Davis

    E-Print Network [OSTI]

    Hernes, Peter J.

    housing.ucdavis.edu Living at UC Davis Residence Halls Living at UC Davis STUDENT HOUSING #12;2 Every year, Student Housing is home to over 6000 UC Davis students, most of whom are first-year freshmen and transfer students who live in the residence halls. Living in Student Housing is not required; however, most

  17. Cooperative self-organization of microorganisms ESHEL BEN-JACOB, INON COHEN

    E-Print Network [OSTI]

    Jacob, Eshel Ben

    of movement according to gradient of chemical agent) signalling, collective activation and deactivation movement and chemotaxis 440 4.3. Bacterial metabolism and growth 442 4.4. Molecular genetics 443 4.5. From

  18. The effects of marine microorganisms on the mechanical properties of graphite/epoxy composites 

    E-Print Network [OSTI]

    Puh, John Shui-Ming

    1997-01-01T23:59:59.000Z

    with two different lay-ups were conditioned in natural seawater and then tensile tested while simultaneously monitored for acoustic emission activity. Graphite/epoxy composite specimens were fabricated from prepreg tape and then conditioned for 4 and I I...

  19. Live Streaming with Gossip Maxime Monod

    E-Print Network [OSTI]

    Guerraoui, Rachid

    ;2 TV ordinaire: tout en HD Live streaming Une source produit du contenu multimédia (un flux) n clients (n large) broadcasting diffusion ... ... ... IP TV, Web TV, P2P TV, ... vs 192K requêtes/jour 78K que le premier #12;·Gossip++Environment contraint ·HEAPEnvironnement hétérogène ·LiFT

  20. Quality health plans & benefits Healthier living

    E-Print Network [OSTI]

    to the Affordable Care Act, also known as the health care reform law, will comply with requirements for WomenQuality health plans & benefits Healthier living Financial well-being Intelligent solutions 05's Preventive Health Services. This means that for women with reproductive capacity, certain women

  1. Hermeneutics by the Living Anton Markos

    E-Print Network [OSTI]

    Markos, Anton

    EDITORIAL Hermeneutics by the Living Anton Markos Received: 20 January 2010 /Accepted: 22 March by introducing hermeneutics? After all, semiotics has already colonized the field, and biosemioticians of different "denominations" flourish at their annual meetings.1 Is not, after all, hermeneutics but an older

  2. living and learning Department of Residential Life

    E-Print Network [OSTI]

    Missouri-Columbia, University of

    living and learning Accessible housing at Mizzou Department of Residential Life University is important to us. The Department of Residential Life at MU is committed to providing and improving accessible spaces for students with disabilities. Residential Life will help provide appropriate housing

  3. Discordance between living and death assemblages as evidence for anthropogenic ecological change

    E-Print Network [OSTI]

    Boyce, C. Kevin

    '' in settings of documented anthropogenic eutrophication (AE) than in areas where AE and other human impacts with eutrophication (anomalous abundance of seagrass-dwellers and/or scarcity of organic-loving species in the death. ecological baseline eutrophication marine communities paleoecology Human activities affect living systems

  4. Detection and cultivation of indigenous microorganisms in Mesozoic claystone core samples from the Opalinus Clay Formation

    E-Print Network [OSTI]

    Gilli, Adrian

    the Opalinus Clay Formation (Mont Terri Rock Laboratory) L. Mauclaire a,*, J.A. McKenzie a , B. Schwyn b , P various deep-subsurface environments, the persistence of microbial activity in clay- stones buried life in the Opalinus Clay Formation (Mesozoic claystone, 170 million years old) at the Mont Terri Rock

  5. NUMERICAL MODELLING OF MICROORGANISMS DISPERSION IN URBAN AREA: APPLICATION TO LEGIONELLA.

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    with transmission of an infectious agent from cooling towers (CT). During the episode that occured in Pas to contaminated cooling towers system may occur over distance larger than 10km. In addition, most cooling towers dispersion from a virtual cooling tower at the same location. The biological model has been activated

  6. Living Rev. Solar Phys., 5, (2008), 3 http://www.livingreviews.org/lrsp-2008-3 in solar physics

    E-Print Network [OSTI]

    Usoskin, Ilya G.

    2008-01-01T23:59:59.000Z

    Living Rev. Solar Phys., 5, (2008), 3 http://www.livingreviews.org/lrsp-2008-3 in solar physics L I V I N G REVIEWS A History of Solar Activity over Millennia Ilya G. Usoskin Sodankyl¨a Geophysical here is a review of present knowledge of the long-term behavior of solar activity on a multi

  7. 25 | P a g e Activity 5

    E-Print Network [OSTI]

    Brownstone, Rob

    · Improved sustainable living and a reduced ecological impact on the environment Background: 30 tips in 30 days will help you incorporate sustainable living practices into your everyday life. Set a personal 25 | P a g e Activity 5 30 Tips in 30 Days Goals: · Motivate sustainable action

  8. Typical College Student Diet Food Derived Microorganisms and Their Relation to the Human Gastrointestinal Microflora

    E-Print Network [OSTI]

    Haselhorst, Alexandria J.

    2011-08-08T23:59:59.000Z

    not yet concrete (11). These probiotics are presently sold commercially in fermented dairy products such as yogurt. Dannon?s Activia? (which contain Bifidus regularis?) and Yakult? (which contains Lactobacillus casei) are examples of such product.... There are reports that in the future, these probiotics may be found in fermented vegetables and meat (11). Prebiotics can be defined as non-digestible food that beneficially affects the host by selectively stimulating the growth and/or activity of bacteria...

  9. UNDERSTANDING QUALITY OF LIFE IN ASSISTED LIVING

    E-Print Network [OSTI]

    UNDERSTANDING QUALITY OF LIFE IN ASSISTED LIVING: INFLUENCE OF THE BUILT AND ORGANIZATIONAL- institutional environment who do not need 24/7 nursing care Focus on quality of life (QoL) 194 registered ALs 6.2 % % Chinese 35.1 51.8 55.6 % South Asian 24.5 26.1 23.5 % Filipino 7.1 5.6 5.7 Total 66.7 83.5 84.9 #12;Our

  10. Fission barriers and half-lives

    SciTech Connect (OSTI)

    Moeller, P.; Nix, J.R.; Swiatecki, W.J.

    1989-01-01T23:59:59.000Z

    We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

  11. Evaluation of terrestrial microcosms for detection, fate, and survival analysis of genetically engineered microorganisms and their recombinant genetic material

    SciTech Connect (OSTI)

    Fredrickson, J.K.; Seidler, R.J.

    1989-02-01T23:59:59.000Z

    The research included in this document represents the current scientific information available regarding the applicability of terrestrial microcosms and related methodologies for evaluating detection methods and the fate and survival of microorganisms in the environment. The three terrestrial microcosms described in this document were used to evaluate the survival and fate of recombinant bacteria in soils and in association with plant surfaces and insects and their transport through soil with percolating water and root systems, and to test new methods and procedures to improve detection and enumeration of bacteria in soil. Simple (potting soil composed of peat mix and perlite, lacking environmental control and monitoring) and complex microcosms (agricultural soil with partial control and monitoring of environmental conditions) were demonstrated to be useful tools for preliminary assessments of microbial viability in terrestrial ecosystems. These studies evaluated the survival patterns of Enterobacter cloacae (pBR322) in soil and on plant surfaces and the ingestion of this same microorganism by cutworms and survival in the foregut and frass. The Versacore microcosm design was used to monitor the fate and competitiveness of genetically engineered bacteria in soil. Both selective media and gene probes were used successfully to follow the fate of two recombinant Pseudomonas sp. introduced into Versacore microcosms. Intact soil-core microcosms were employed to evaluate the fate and transport of genetically altered Azospirillum sp. and Pseudomonas sp. in soil and the plant rhizosphere. The usefulness of these various microcosms as a tool for risk assessment is underscored by the ease in obtaining soil from a proposed field release site to evaluate subsequent GEM fate and survival.

  12. Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions

    SciTech Connect (OSTI)

    Reed, Donald Timothy [Los Alamos National Laboratory; Borkowski, Marian [Los Alamos National Laboratory; Lucchini, Jean - Francois [Los Alamos National Laboratory; Ams, David [Los Alamos National Laboratory; Richmann, M. K. [Los Alamos National Laboratory; Khaing, H. [Los Alamos National Laboratory; Swanson, J. S. [Los Alamos National Laboratory

    2010-12-10T23:59:59.000Z

    The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

  13. Solar Decathlon Team Leading the Way Toward Sustainable Living...

    Office of Environmental Management (EM)

    Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters August...

  14. Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte

    E-Print Network [OSTI]

    Bertini, Robert L.

    Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University Oregon Transportation Summit Sept 10, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money Official transportation data archive for the Portland-Vancouver metropolitan region

  15. Search for Short Lived Particles in High Multiplicity Environment

    E-Print Network [OSTI]

    Marek Gazdzicki; Waldemar Retyk; Jan Pluta

    1999-04-14T23:59:59.000Z

    A method of statistical selection of short lived particles in high multiplicity nucleus-nucleus collisions is discussed.

  16. AN EARLY WARNING SYSTEM FOR AMBIENT ASSISTED LIVING

    E-Print Network [OSTI]

    Karlsson, Brynjar

    AN EARLY WARNING SYSTEM FOR AMBIENT ASSISTED LIVING June 2012 Andrea Monacchi Master of Science in Computer Science #12;#12;AN EARLY WARNING SYSTEM FOR AMBIENT ASSISTED LIVING Andrea Monacchi Master ISSN 1670-8539 #12;#12;An Early Warning System for Ambient Assisted Living by Andrea Monacchi Project

  17. Software Product Line Engineering for Long-lived, Sustainable Systems

    E-Print Network [OSTI]

    Lutz, Robyn R.

    Software Product Line Engineering for Long-lived, Sustainable Systems Robyn Lutz1,2, David Weiss1 system, long-lived system, variability, commonality/variability analysis. 1 Introduction Sustainable and requirements, distinguishes sustainable systems from legacy systems. Accordingly we use the term long-lived

  18. Student Accommodation and Living Guide 2012/2013

    E-Print Network [OSTI]

    Zharkova, Valentina V.

    to sustainable living that has won our campus the award for `Outstanding Contribution to Sustainable DevelopmentStudent Accommodation and Living Guide 2012/2013 #12;For Further Information: Tel: (0)1274 234883 STUDENT ACCOMMODATION AND LIVING GUIDE 2012-2013 City Campus 2 The City of Bradford 4 City Campus Map 6

  19. SEMINOLES CITYINTHE YOUR GUIDE TO TALLAHASSEE & OFF-CAMPUS LIVING

    E-Print Network [OSTI]

    McQuade, D. Tyler

    roommate and neighbor relationships, and develop a sustainable style of living while in TallahasseeSEMINOLES CITYINTHE YOUR GUIDE TO TALLAHASSEE & OFF-CAMPUS LIVING 2013 #12;#12;Seminole Creed and living in the University and beyond. Freedom of Speech and Inquiry I will support academic freedom

  20. Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte

    E-Print Network [OSTI]

    Bertini, Robert L.

    Intelligent Transportation Systems: Saving Lives, Time and Money Kristin Tufte Portland State University June 23, 2010 #12;Intelligent Transportation Systems: Saving Lives, Time and Money 1,400,000 urban cities 3 counties 1 region #12;Intelligent Transportation Systems: Saving Lives, Time and Money Why Now

  1. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only once is a trick. If you can use of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only

  2. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana, 2010 Math 499, March 2, 2011 Bruce Reznick University of Illinois at Urbana-Champaign The secret lives-Champaign The secret lives of polynomial identities #12;"An idea which can be used only once is a trick. If you can use

  3. (Catalytic mechanism of hydrogenase from aerobic N sub 2 -fixing microorganisms)

    SciTech Connect (OSTI)

    Arp, D.J.

    1991-01-01T23:59:59.000Z

    The results of this DOE-sponsored project have contributed to our understanding of the catalytic mechanism of A. vinelandii hydrogenase. A group of inhibitors have been characterized. These provide information about the different types of redox clusters involved in catalysis and the roles of each. One group has already used acetylene in a study of three desulfovibrian hydrogenases and shown that only the NiFe hydrogenases are inhibited. We have characterized a number of spectral properties of A. vinelandii hydrogenase. The EPR signals associated with this hydrogenase in the reduced state are reminiscent of other NiFe dimeric hydrogenases such as A. eutrophus, but distinctly difference from others such as D. gigas and Chromatium vinosum. Thus, while the NiFe dimeric hydrogenases are now recognized as a large group of similar enzymes, there are differences in the spectral and catalytic properties which are not explained by their similar redox inventories, identical subunit structures, immunological cross reactivity and conserved sequences. The inhibitors we have characterized are also proving of value in the spectral characterizations. Surprisingly, we only see a significant EP signal attributable to Ni after the enzyme has been inactivated with O{sub 2} and then reduced (though not reactivated). No spectral perterbations (EPR or UV-V is) of active enzyme can be attributed to binding of H{sub 2}, even though H{sub 2} clearly binds to this form of the enzyme. Acetylene, which does not substantially perterb the EPR signal of active hydrogenase, does result in a new absorption envelope in the UV-V is spectrum. Overall, the results of this project have revealed the complex interactions of the redox clusters in catalysis through studies of inhibitor mechanisms and spectral properties. 14 refs., 9 figs.

  4. Proton radioactivity half lives with Skyrme interactions

    E-Print Network [OSTI]

    T. R. Routray; Abhishek Mishra; S. K. Tripathy; B. Behera; D. N. Basu

    2012-05-31T23:59:59.000Z

    The potential barrier impeding the spontaneous emission of protons in the proton radioactive nuclei is calculated as the sum of nuclear, Coulomb and centrifugal contributions. The nuclear part of the proton-nucleus interaction potential is obtained in the energy density formalism using Skyrme effective interaction that results into a simple algebraic expression. The half-lives of the proton emitters are calculated for the different Skyrme sets within the improved WKB framework. The results are found to be in reasonable agreement with the earlier results obtained for more complicated calculations involving finite range interactions.

  5. ARM - General Changes in Daily Lives

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006Datastreamstwrcam40m DocumentationJanuary 9, 2009 [Events, FeatureListGeneral Changes in Daily Lives Outreach

  6. Live pathogens: rapid detection technique developed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -ChoicesListLive Discussion

  7. Community is Live!! | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual Siteof EnergyInnovationin UrbanCityCoatedCommunity ElectricPreliminaryCommunity is Live!!

  8. Rural Living Canada Website | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are beingZealand Jump to:Ezfeedflag JumpID-f < RAPID‎ |Rippey Jump to:WY) Jump to:Rosneft JumpRugbyLiving Canada

  9. Living Walls | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are being directedAnnual SiteofEvaluatingGroup |Jilin ZhongdiantouLichuan CityLiqcrytech LLCLiuzhou MinghuiLiving Walls

  10. Physiologically anaerobic microorganisms of the deep subsurface. Final performance report, June 1, 1990--August 31, 1993

    SciTech Connect (OSTI)

    Stevens, S.E. Jr.; Chung, K.T.

    1993-10-01T23:59:59.000Z

    Anaerobic bacteria were isolated from deep subsurface sediment samples taken at study sites in Idaho (INEL) and Washington (HR) by culturing on dilute and concentrated medium. Morphologically distinct colonies were purified, and their responses to 21 selected physiological tests were determined. Although the number of isolates was small (18 INEL, 27 HR) some general patterns could be determined. Most strains could utilize all the carbon sources, however the glycerol and melizitose utilization was positive for 50% or less of the HR isolates. Catalase activity (27.78% at INEL, 74.07% at HR) and tryptophan metabolism (11.12% at INEL, 40.74% at HR) were significantly different between the two study sites. MPN and viable counts indicate that sediments near the water table yield the greatest numbers of anaerobes. Deeper sediments also appear to be more selective with the greatest number of viable counts on low-nutrient mediums. Likewise, only strictly obligate anaerobes were found in the deepest sediment samples. Selective media indicated the presence of methanogens, acetogens, and sulfate reducers at only the HR site.

  11. Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms

    DOE Patents [OSTI]

    Seibert, Michael (Lakewood, CO); Makarova, Valeriya (Golden, CO); Tsygankov, Anatoly A. (Pushchino, RU); Rubin, Andrew B. (Moscow, RU)

    2007-06-12T23:59:59.000Z

    In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.

  12. antibody-positive women living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Kenong Wu; David Gauthier; Martin D. Levine 1993-01-01 128 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  13. Annual report and summaries of FY 1994 activities

    SciTech Connect (OSTI)

    Not Available

    1994-09-01T23:59:59.000Z

    The Energy Biosciences program was initiated in 1979 for the purpose of fulfilling the need of basic information about plants and microorganisms relating to varied energy matters. The program generates basic information that contributes significantly to future technologies involving alternate fuel generation, petroleum replacements, sustained industrial activities along with means of improving environmental conditions. The Energy Biosciences program during Fiscal Year 1994 received 139 new research applications following the screening of numerous preapplications. Of the applications received, 21 projects were funded.

  14. Living anionic polymerization using a microfluidic reactor

    SciTech Connect (OSTI)

    Iida, Kazunori; Chastek, Thomas Q.; Beers, Kathryn L.; Cavicchi, Kevin A.; Chun, Jaehun; Fasolka, Michael J.

    2009-02-01T23:59:59.000Z

    Living anionic polymerizations were conducted within aluminum-polyimide microfluidic devices. Polymerizations of styrene in cyclohexane were carried out at various conditions, including elevated temperature (60 °C) and high monomer concentration (42%, by volume). The reactions were safely maintained at a controlled temperature at all points in the reactor. Conducting these reactions in a batch reactor results in uncontrolled heat generation with potentially dangerous rises in pressure. Moreover, the microfluidic nature of these devices allows for flexible 2D designing of the flow channel. Four flow designs were examined (straight, periodically pinched, obtuse zigzag, and acute zigzag channels). The ability to use the channel pattern to increase the level of mixing throughout the reactor was evaluated. When moderately high molecular mass polymers with increased viscosity were made, the patterned channels produced polymers with narrower PDI, indicating that passive mixing arising from the channel design is improving the reaction conditions.

  15. Living and Working in the Freezer

    SciTech Connect (OSTI)

    Hill, Victoria (Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity) [Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity

    2012-02-07T23:59:59.000Z

    Very little data of any kind exists from the early spring in the Arctic. The reason? It's extremely cold and that makes it difficult to survive, let alone conduct science. From March through the end of April, 2011, scientists from around the world braved temperatures of -48?C in the high Canadian Arctic in the name of science. At the Catlin Arctic Survey's floating 'Ice Base' off Ellef Ringnes Island, Dr. Victoria Hill was investigating how organic material in fresh water near the surface of the ocean may be trapping heat from the sun, causing the upper ocean layers to warm. This is a very new area of research and this mechanism represents a key uncertainty in accurate modeling of ice thickness and upper ocean heat content. In this presentation Dr. Hill will talk about living and working at the ice base and discuss preliminary data from the expedition.

  16. Slow urban living apartments : transformation of five story walk-up apartments in Seoul

    E-Print Network [OSTI]

    Lim, Yihyun

    2014-01-01T23:59:59.000Z

    Experiential living is the new trend for future living. Whether it is through living in micro spaces, flexible units, mixed-use developments, practicing urban farming, or sharing lifestyles, these different trends of living ...

  17. Reversible CO-binding to the Active Site of Nitrogenase | Stanford...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Reversible CO-binding to the Active Site of Nitrogenase Tuesday, March 31, 2015 All living organisms depend on the availability of nitrogen for incorporation into the basic...

  18. Scientists capture 'redox moments' in living cells | EMSL

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    moments' in living cells Better understanding of hardy bacteria enhances tool for biofuel creation Scientists have charted a significant signaling network in a tiny organism...

  19. Sandia National Laboratories: ensure we have a living river

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    ensure we have a living river Sandia, the Atlantic Council, and NM Water Resource Research Institute Sponsor Roundtable on Western Water Scarcity On October 4, 2013, in Climate,...

  20. aboriginal children living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    resources appropriately 12;Aboriginal people - the first environmentalists Truly sustainable living (Zero work with the Nyungar community to not only continue, but grow...

  1. assisted living facilities: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    related field o have experience working in an educational setting o Sheridan, Jennifer 15 Sustainable Living Facilities (SLF) Requirements, Expectations, Pledge and Agreement...

  2. asian communities living: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Colleges within large Universities Barrash, Warren 7 University Housing Designing Sustainable Communities for Living and Learning Biology and Medicine Websites Summary: The...

  3. Brownsville Public Utilities Board- Green Living Residential Rebate Program

    Broader source: Energy.gov [DOE]

    Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

  4. Dutch Company Powers Streetlights With Living Plants; Will Your...

    Open Energy Info (EERE)

    Dutch Company Powers Streetlights With Living Plants; Will Your Cell Phone Be Next? Home > Groups > OpenEI Community Central Dc's picture Submitted by Dc(266) Contributor 16...

  5. Mass Spectral Molecular Networking of Living Microbial Colonies...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of Living Microbial Colonies. Abstract: Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a "holy grail" in...

  6. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

    1993-08-01T23:59:59.000Z

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

  7. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

    2007-12-04T23:59:59.000Z

    The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  8. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

    2007-10-30T23:59:59.000Z

    The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  9. Why we live in 3 Dimensions

    E-Print Network [OSTI]

    Carlos Castro; Alex Granik; M. S. El Naschie

    2000-08-18T23:59:59.000Z

    A Cantorian fractal spacetime, a family member of von Neumann's noncommutative geometry is introduced as a geometry underlying a new relativity theory which is similar to the relation between general relativity and Riemannian geometry. Based on this model and the new relativity theory an ensemble distribution of all the dimensions of quantum spacetime is derived with the help of Fermat grand theorem. The calculated average dimension is very close to the value of $4+\\phi^3 $ (where $\\phi$ is the golden mean) obtained by El Naschie on the basis of a different approach. It is shown that within the framework of the new relativity the cosmological constant problem is nonexistent, since the Universe self-organizes and self-tunes according to the renormalization group (RG) flow with respect to a local scaling microscopic arrow of time. This implies that the world emerged as a result of a non-equilibrium process of self-organized critical phenomena launched by vacuum fluctuations in Cantorian fractal spacetime $\\cal E^{\\infty}$. It is shown that we are living in a metastable vacuum and are moving towards a fixed point ($ D$ = 4+$\\phi^3$) of the RG. After reaching this point, a new phase transition will drive the universe to a quasi-crystal phase of the lower average dimension of $\\phi^3$.

  10. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2010-06-15T23:59:59.000Z

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  11. Genetically modified microorganisms for

    E-Print Network [OSTI]

    Angenent, Lars T.

    -proteins. Thus far, Shewanella oneidensis and Geobacter sulfurreducens have been extensively studied into electricity in much more efficient ways. This chapter will discuss recent work on the investigation be possible in the near future. 6.2 EXTRACELLULAR RESPIRATION IN SHEWANELLA ONEIDENSIS AND GEOBACTER

  12. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2007-01-09T23:59:59.000Z

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  13. housing.ucdavis.edu Living at UC Davis

    E-Print Network [OSTI]

    Hernes, Peter J.

    housing.ucdavis.edu Living at UC Davis Apartments Living at UC Davis STUDENT HOUSING #12;2 Every year, Student Housing is home to over 6000 UC Davis students, over 500 of whom are transfer students and students participating in graduate and professional programs. UC Davis also provides housing that is ideal

  14. an interview with Richard Alba Extraordinary Lives: Patti Smith

    E-Print Network [OSTI]

    Dennehy, John

    When racial boundaries fade an interview with Richard Alba Extraordinary Lives: Patti Smith What on the workforce, the family, and American society. EXTRAORDINARY LIVES: PATTI SMITH 12 This was the first featured the maximally talented Patti Smith--singer, songwriter, artist and poet--who spoke thoughtfully

  15. Yugoslavia is DeaD long live the Yugosphere

    E-Print Network [OSTI]

    Neirotti, Juan Pablo

    Tim Judah Yugoslavia is DeaD long live the Yugosphere tim JuDah Good news from the Western Balkans JuDah Good news from the Western Balkans #12;Yugoslavia is Dead . . . Long Live the Yugosphere LSEE happened. In that case how can they react properly? How can we correctly analyse the Western Balkans

  16. Transportation Agency Tool to Analyze Benefits of Living Snow Fences

    E-Print Network [OSTI]

    Minnesota, University of

    Transportation Agency Tool to Analyze Benefits of Living Snow Fences 5/31/12 Transportation Agency/31/12 Transportation Agency Tool to Analyze Benefits of Living Snow Fences Center for Integrated Natural Resources, Mobility, & Transportation Authority Benefits, Farmer Costs, & Carbon Impacts Focus Groups and Surveys

  17. Carbon nanotubes as photoacoustic molecular imaging agents in living mice

    E-Print Network [OSTI]

    Khuri-Yakub, Butrus T. "Pierre"

    Carbon nanotubes as photoacoustic molecular imaging agents in living mice ADAM DE LA ZERDA1 not shown to target a diseased site in living subjects. Here we show that single-walled carbon nanotubes were verified ex vivo using Raman microscopy. Photoacoustic imaging of targeted single-walled carbon

  18. "Space Station" Theme: Learning to Work, and Live, in Space

    E-Print Network [OSTI]

    Mathis, Wayne N.

    "Space Station" IMAX Film Theme: Learning to Work, and Live, in Space The educational value of NASM visit and afterward. See the "Alignment with Standards" table for details regarding how "Space Station in the "Space Station" program: · How astronauts train · What it is like to live and work in Space aboard

  19. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana-Champaign University of Washington May 18, 2012 Bruce Reznick University of Illinois at Urbana-Champaign The secret of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only

  20. The secret lives of polynomial identities Bruce Reznick

    E-Print Network [OSTI]

    Reznick, Bruce

    The secret lives of polynomial identities Bruce Reznick University of Illinois at Urbana-Champaign University of Konstanz June 30, 2011 Bruce Reznick University of Illinois at Urbana-Champaign The secret of Illinois at Urbana-Champaign The secret lives of polynomial identities #12;"An idea which can be used only

  1. basic student budget 2014 |2015 LIVING ON CAMPUS

    E-Print Network [OSTI]

    Painter, Kevin

    basic student budget 2014 |2015 #12;LIVING ON CAMPUS HALL FEES £ 119.18 ­ average cost x 40 weeks at £119.18 per week, and may be cheaper depending on type of accommodation. This budget is meant months (whether you live in the flat over the summer or not). This budget is meant as a realistic guide

  2. Promoting Balance, Wellness & Fitness Creating healthier lives.

    E-Print Network [OSTI]

    Washington at Seattle, University of

    in student recruitment and retention. Engagement ­ We provide opportunities for students and members of their leisure time. Participation in such activities also assists students in performing well in a demanding interpersonal conflicts, learn healthy life-style habits, provide first aid and emergency response services

  3. Howell, R.A., 2012 Living with a carbon allowance 1 Living with a carbon allowance: the experiences of Carbon

    E-Print Network [OSTI]

    Howell, R.A., 2012 Living with a carbon allowance 1 Living with a carbon allowance: the experiences of Carbon Rationing Action Groups and implications for policy Rachel A. Howell Environmental Change with a carbon allowance: the experiences of Carbon Rationing Action Groups and implications for policy. Energy

  4. 9/4/12 Tigers Work the Night Shift in Nepal | LiveScience 1/8www.livescience.com/22876-tigers-humans-coexist.html

    E-Print Network [OSTI]

    9/4/12 Tigers Work the Night Shift in Nepal | LiveScience 1/8www.livescience.com/22876-tigers Shift in Nepal #12;9/4/12 Tigers Work the Night Shift in Nepal | LiveScience 2/8www.livescience.com/22876-tigers-humans-coexist.html A Bengal tiger caught by a motion-activated camera in Nepal. CREDIT

  5. Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus

    E-Print Network [OSTI]

    Steglich, Claudia

    Background RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, a ...

  6. Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus

    E-Print Network [OSTI]

    Steglich, Claudia

    Background: RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, ...

  7. Harnessing microbial subsurface metal reduction activities to synthesise nanoscale cobalt ferrite with enhanced magnetic properties

    SciTech Connect (OSTI)

    Coker, Victoria S.; Telling, Neil D.; van der Laan, Gerrit; Pattrick, Richard A.D.; Pearce, Carolyn I.; Arenholz, Elke; Tuna, Floriana; Winpenny, Richard E.P.; Lloyd, Jonathan R.

    2009-03-24T23:59:59.000Z

    Nanoscale ferrimagnetic particles have a diverse range of uses from directed cancer therapy and drug delivery systems to magnetic recording media and transducers. Such applications require the production of monodisperse nanoparticles with well-controlled size, composition, and magnetic properties. To fabricate these materials purely using synthetic methods is costly in both environmental and economical terms. However, metal-reducing microorganisms offer an untapped resource to produce these materials. Here, the Fe(III)-reducing bacterium Geobacter sulfurreducens is used to synthesize magnetic iron oxide nanoparticles. A combination of electron microscopy, soft X-ray spectroscopy, and magnetometry techniques was employed to show that this method of biosynthesis results in high yields of crystalline nanoparticles with a narrow size distribution and magnetic properties equal to the best chemically synthesized materials. In particular, it is demonstrated here that cobalt ferrite (CoFe{sub 2}O{sub 4}) nanoparticles with low temperature coercivity approaching 8 kOe and an effective anisotropy constant of {approx} 10{sup 6} erg cm{sup -3} can be manufactured through this biotechnological route. The dramatic enhancement in the magnetic properties of the nanoparticles by the introduction of high quantities of Co into the spinel structure represents a significant advance over previous biomineralization studies in this area using magnetotactic bacteria. The successful production of nanoparticulate ferrites achieved in this study at high yields could open up the way for the scaled-up industrial manufacture of nanoparticles using environmentally benign methodologies. Production of ferromagnetic nanoparticles for pioneering cancer therapy, drug delivery, chemical sensors, catalytic activity, photoconductive materials, as well as more traditional uses in data storage embodies a large area of inorganic synthesis research. In particular, the addition of transition metals other than Fe into the structure of magnetite (Fe{sub 3}O{sub 4}) has been shown to greatly enhance the magnetic properties of the particles, tailoring them to different commercial uses. However, synthesis of magnetic nanoparticles is often carried out at high temperatures with toxic solvents resulting in high environmental and energy costs. Additionally, these ferrite nanoparticles are not intrinsically biocompatible, and to make them suitable for insertion into the human body is a rather intricate task. A relatively unexplored resource for magnetic nanomaterial production is subsurface Fe(III)-reducing bacteria, as these microorganisms are capable of producing large quantities of nanoscale magnetite (Fe{sub 3}O{sub 4}) at ambient temperatures. Metal-reducing bacteria live in environments deficient in oxygen and conserve energy for growth through the oxidation of hydrogen or organic electron donors, coupled to the reduction of oxidized metals such as Fe(III)-bearing minerals. This can result in the formation of magnetite via the extracellular reduction of amorphous Fe(III)-oxyhydroxides causing the release of soluble Fe(II) and resulting in complete recrystallization of the amorphous mineral into a new phase. Some previous studies have reported altering the composition of biogenic magnetite produced by Fe(III)-reducing bacteria for industrial and environmental applications. However, research into the commercial exploitation of bacteria to form magnetic minerals has focused primarily on magnetotactic bacteria which form magnetosomal magnetite internally using very different pathways to those bacteria forming magnetite outside the cell. Magnetotactic bacteria live at the sediment-water interface and use internal nanomagnets to guide them to their preferred environmental niche using the Earth's magnetic field. Since magnetotactic bacteria generally grow optimally under carefully controlled microaerobic conditions, the culturing processes for these organisms are challenging and result in low yields of nanomagnetite. Despite these limitations, magnetotactic bacteria have bee

  8. Long lived central engines in Gamma Ray Bursts

    E-Print Network [OSTI]

    G. Ghisellini

    2008-10-01T23:59:59.000Z

    The central engine of Gamma Ray Bursts may live much longer than the duration of the prompt emission. Some evidence of it comes from the presence of strong precursors, post-cursors, and X-ray flares in a sizable fraction of bursts. Additional evidence comes from the fact that often the X-ray and the optical afterglow light curves do not track one another, suggesting that they are two different emission components. The typical "steep-flat-steep" behavior of the X-ray light curve can be explained if the same central engine responsible for the main prompt emission continues to be active for a long time, but with a decreasing power. The early X-ray "afterglow" emission is then the extension of the prompt emission, originating at approximately the same location, and is not due to forward shocks. If the bulk Lorentz factor Gamma is decreasing in time, the break ending the shallow phase can be explained, since at early times Gamma is large, and we see only a fraction of the emitting area. Later, when Gamma decreases, we see an increasing fraction of the emitting surface up to the time when Gamma ~ 1/theta_j. This time ends the shallow phase of the X-ray light curve. The origin of the late prompt emission can be the accretion of the fall-back material, with an accretion rate dot M proportional to t^(-5/3). The combination of this late prompt emission with the flux produced by the standard forward shock can explain the great diversity of the optical and the X-ray light curves.

  9. 'Invisible lives' : the Gypsies and Travellers of Britain 

    E-Print Network [OSTI]

    Clark, Colin Robert

    2001-12-05T23:59:59.000Z

    This thesis examines the lives and experiences of relatively 'unknown' minority ethnic communities in Britain. As a group, they have been known by many names since their arrival in the 15th Century, although the most common ...

  10. Oak Leaf Roller and Springtime Defoliation of Live Oak Trees

    E-Print Network [OSTI]

    Drees, Bastiaan M.

    2004-03-26T23:59:59.000Z

    This publication explains how to minimize damage to live oak trees by the oak leaf roller and an associated caterpillar species, which occur throughout Texas but are most damaging in the Hill Country and South Texas....

  11. Our Energy Independence- A Live Chat With Dr. Arun Majumdar

    Broader source: Energy.gov [DOE]

    Join Dr. Arun Majumdar at 2 PM ET for a live, two-way conversation about the investments we're making to build the clean energy infrastructure of the future.

  12. Live Webcast on Recent Wind Energy Technology Advances

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  13. Live long and prosper: Germline stem cell maintenance

    E-Print Network [OSTI]

    Extavour, Cassandra

    Live long and prosper: Germline stem cell maintenance revisited (retrospective on DOI: 10.1002/bies aging is rapid or their divisions tend to produce game- togenic daughters, then they may limit animals

  14. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

    1991-01-01T23:59:59.000Z

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

  15. Heavy Long-lived Mossbauer State of Niobium

    E-Print Network [OSTI]

    Cheng, Yao

    2011-01-01T23:59:59.000Z

    A heavy niobium state showing 1/3 residual resistance is discovered below the superconducting transition temperature. This niobium sample contains high-density long-lived Mossbauer excitation.

  16. Heavy Long-lived Mossbauer State of Niobium

    E-Print Network [OSTI]

    Yao Cheng; Chi-Hao Lee

    2011-03-03T23:59:59.000Z

    A heavy niobium state showing 1/3 residual resistance is discovered below the superconducting transition temperature. This niobium sample contains high-density long-lived Mossbauer excitation.

  17. The living commons : a spatial theory for biological design

    E-Print Network [OSTI]

    Telhan, Orkan

    2013-01-01T23:59:59.000Z

    Biological design is as ancient as human civilization. For thousands of years, living systems and natural processes have been manipulated by humans and their biological outcomes have been customized for different purposes. ...

  18. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Broader source: Energy.gov (indexed) [DOE]

    December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D....

  19. University Housing Designing Sustainable Communities for Living and Learning

    E-Print Network [OSTI]

    Almor, Amit

    The Mission Learning Center for Sustainable Futures Green Learning Community Student Engagement Green Living Public Events Green Education University and Community Outreach Community Building Our Home:Physical Features LEED Certification Sustainable Sites Materials and Resources Energy and Atmosphere Indoor

  20. Development of a Multiplexed, Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples. Final Report

    SciTech Connect (OSTI)

    Lowe, M.; Halden, R.

    2002-10-09T23:59:59.000Z

    This was the final report for DOE NABIR grant DE-FG02-01ER63264 (PI Mary Lowe). The grant was entitled ''Development of a Multiplexed Bead-Based Assessment Tool for Rapid Identification and Quantitation of Microorganisms in Field Samples.'' The grant duration was one year. The purpose was to develop a bead-based assay for measuring analyte DNAs in environmental PCR products and to apply the method to a field experiment. The primary experiment was located at the UMTRA Old Rifle site.

  1. Power Spectrum of Out-of-equilibrium Forces in Living Cells : Amplitude and Frequency Dependence

    E-Print Network [OSTI]

    Francois Gallet; Delphine Arcizet; Pierre Bohec; Alain Richert

    2009-01-20T23:59:59.000Z

    Living cells exhibit an important out-of-equilibrium mechanical activity, mainly due to the forces generated by molecular motors. These motor proteins, acting individually or collectively on the cytoskeleton, contribute to the violation of the fluctuation-dissipation theorem in living systems. In this work we probe the cytoskeletal out-of-equilibrium dynamics by performing simultaneous active and passive microrheology experiments, using the same micron-sized probe specifically bound to the actin cortex. The free motion of the probe exhibits a constrained, subdiffusive behavior at short time scales (t power law dependence with time. Combining the results of both experiments, we precisely measure for the first time the power spectrum of the force fluctuations exerted on this probe, which lies more than one order of magnitude above the spectrum expected at equilibrium, and greatly depends on frequency. We retrieve an effective temperature Teff of the system, as an estimate of the departure from thermal equilibrium. This departure is especially pronounced on long time scales, where Teff bears the footprint of the cooperative activity of motors pulling on the actin network. ATP depletion reduces the fluctuating force amplitude and results in a sharp decrease of Teff towards equilibrium.

  2. LiveWall Operational Evaluation: Seattle Law Enforcement Pilot

    SciTech Connect (OSTI)

    Barr, Jonathan L.; Burtner, Edwin R.; Stein, Steven L.

    2013-10-01T23:59:59.000Z

    The LiveWall concept envisioned as an outgrowth of the Precision Information Environment (PIE) project allows communications between separate groups using interactive video, audio, and a shared desktop environment; this allows everyone to participate and collaborate in real time, regardless of location. The LiveWall concept provides a virtual window to other locations, where all parties can interact and collaboratively work with each other. This functionality is intended to improve multi-site coordination amongst emergency operations centers (EOC), field operations sites and across organizations and jurisdictions to accommodate communications during routine and emergency events. For the initial LiveWall operational evaluation PNNL partnered with the Seattle Police Department (SPD). This partnership allowed for the creation of an excellent LiveWall test bed specific to law enforcement. This partnership made it possible to test the LiveWall concept with scenarios involving the many facets of the law enforcement work done by SPD. PNNL and SPD agreed that integrating the systems into operations for a real event would be the best test of the technology and give SPD staff greater visibility into the functionality and benefits offered by the LiveWall concept.

  3. adult-to-adult living donor: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D. G. Allen; C. R. Stanley; M. S. Sherwin 2005-03-05 92 Developing solutions for sustainable living-the Urban Living Laboratory: The world's largest 'living laboratory'...

  4. Long-Range Untethered Real-Time Live Gas Main Robotic Inspection System

    SciTech Connect (OSTI)

    Hagen Schempf; Daphne D'Zurko

    2004-10-31T23:59:59.000Z

    Under funding from the Department of Energy (DOE) and the Northeast Gas Association (NGA), Carnegie Mellon University (CMU) developed an untethered, wireless remote controlled inspection robot dubbed Explorer. The project entailed the design and prototyping of a wireless self-powered video-inspection robot capable of accessing live 6- and 8-inch diameter cast-iron and steel mains, while traversing turns and Ts and elbows under real-time control with live video feedback to an operator. The design is that of a segmented actively articulated and wheel-leg powered robot design, with fisheye imaging capability and self-powered battery storage and wireless real-time communication link. The prototype was functionally tested in an above ground pipe-network, in order to debug all mechanical, electrical and software subsystems, and develop the necessary deployment and retrieval, as well as obstacle-handling scripts. A pressurized natural gas test-section was used to certify it for operation in natural gas at up to 60 psig. Two subsequent live-main field-trials in both cast-iron and steel pipe, demonstrated its ability to be safely launched, operated and retrieved under real-world conditions. The system's ability to safely and repeatably exidrecover from angled and vertical launchers, traverse multi-thousand foot long pipe-sections, make T and varied-angle elbow-turns while wirelessly sending live video and handling command and control messages, was clearly demonstrated. Video-inspection was clearly shown to be a viable tool to understand the state of this critical buried infrastructure, irrespective of low- (cast-iron) or high-pressure (steel) conditions. This report covers the different aspects of specifications, requirements, design, prototyping, integration and testing and field-trialing of the Explorer platform.

  5. Life in the Living Laboratory: An Anthropological Investigation of Environmental Science, Tourism, and Design in the Contemporary Bahamas

    E-Print Network [OSTI]

    Moore, Amelia M.

    2010-01-01T23:59:59.000Z

    envisioned through sustainable designs for living. It can bethe living laboratory- biological, sustainable, ecologicalsustainable economic development- processes of human life and living,

  6. Microbial metaproteomics: Identifying the repertoire of proteins that microorganisms use to compete and cooperate in complex environmental communities

    SciTech Connect (OSTI)

    Hettich, Robert {Bob} L [ORNL; Sharma, Ritin [ORNL; Chourey, Karuna [ORNL; Giannone, Richard J [ORNL

    2012-01-01T23:59:59.000Z

    The availability of genome information for microbial consortia, including unculturable species, from environmental samples has enabled systems-biology interrogation by providing a means to access genomic, transcriptomic, and proteomic information. This provides a unique opportunity to characterize the molecular activities and interactions of these microbial systems at a comprehensive level never before possible. Such information not only provides details about the organizational, functional, and metabolic activities of such systems, but also the untapped reserve of molecular activities that might be invoked and exploited under certain environmental conditions. Since bacteria naturally exist in complex ecosystems, it is imperative to develop and utilize analytical approaches that can provide molecular level details on systems consisting of mixed microbial membership. This is the realm of metaproteomics - the characterization of the complement of proteins expressed by a microbial community in an environmental sample

  7. The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living a core experience for First

    E-Print Network [OSTI]

    The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living University. The Sustainability community infuses the learning-living community with practical and theoretical approaches to sustainable living, merging students' living community with unique academic and field

  8. Uncharted Microbial World: Microbes and Their Activities in the Environment

    SciTech Connect (OSTI)

    Harwood, Caroline; Buckley, Merry.

    2007-12-31T23:59:59.000Z

    Microbes are the foundation for all of life. From the air we breathe to the soil we rely on for farming to the water we drink, everything humans need to survive is intimately coupled with the activities of microbes. Major advances have been made in the understanding of disease and the use of microorganisms in the industrial production of drugs, food products and wastewater treatment. However, our understanding of many complicated microbial environments (the gut and teeth), soil fertility, and biogeochemical cycles of the elements is lagging behind due to their enormous complexity. Inadequate technology and limited resources have stymied many lines of investigation. Today, most environmental microorganisms have yet to be isolated and identified, let alone rigorously studied. The American Academy of Microbiology convened a colloquium in Seattle, Washington, in February 2007, to deliberate the way forward in the study of microorganisms and microbial activities in the environment. Researchers in microbiology, marine science, pathobiology, evolutionary biology, medicine, engineering, and other fields discussed ways to build on and extend recent successes in microbiology. The participants made specific recommendations for targeting future research, improving methodologies and techniques, and enhancing training and collaboration in the field. Microbiology has made a great deal of progress in the past 100 years, and the useful applications for these new discoveries are numerous. Microorganisms and microbial products are now used in industrial capacities ranging from bioremediation of toxic chemicals to probiotic therapies for humans and livestock. On the medical front, studies of microbial communities have revealed, among other things, new ways for controlling human pathogens. The immediate future for research in this field is extremely promising. In order to optimize the effectiveness of community research efforts in the future, scientists should include manageable systems with features like clear physical boundaries, limited microbial diversity, and manipulability with the goal of understanding fundamental principles that may apply to more complex systems. A great deal of microbial genetic and phenotypic diversity remains to be explored, and the commercial and medical potential locked up in these unknowns should compel the field to move forward. Future microbiology research will build on the successes of the past using new techniques and approaches. Uncultivated microbes hold great promise for industry, medicine, and the recycling of precious resources, and research and technology must make inroads in overcoming the barriers that prevent their study. In many cases, we will no longer be able to rely on isolated, pure cultures of microorganisms, but must use communities of microorganisms, which presently are poorly understood. Indeed, community-level studies can benefit from deconstructing microbial communities and analyzing the component members separately, but this is not feasible in every system. The effects of perturbation on microbial communities also require study. Humans rely on the services of microbes in innumerable ways, but we have little or no predictive understanding of how microbial communities respond to disturbance. Research must address current limitations in detecting microscale interactions among microbes by enhancing current technologies and fostering new microscopic tools, biosensors, and gas sensors for appropriate small scales. Genomics, which has enabled great progress in microbiology research of individual species, must be applied to communities of microorganisms. This will require improved methods of DNA extraction and amplification from environmental samples and improved strategies for DNA sequence assembly. In the future, genome sequencing efforts should continue the exploration of evolutionarily diverse microbes, as well as help reveal the mechanisms by which closely related microbes evolve. Technological advances have spurred every great leap in microbial biology, and in order to move forward, new m

  9. The Sun is an active, variable,

    E-Print Network [OSTI]

    Christian, Eric

    The Sun is an active, variable, magnetic star with oscillations on its surface and deep within its the physical processes of the Sun-Earth connection. Visit Us on the Web: Solar Terrestrial Probes Program: http://stp.gsfc.nasa.gov For More Information, See: Sun-Earth Connection: http://sec.gsfc.nasa.gov Living With a Star: http

  10. 27 | P a g e Activity 6

    E-Print Network [OSTI]

    Brownstone, Rob

    sustainable living and reduced ecological impacts · An increased sense of community and the creation 27 | P a g e Activity 6 The Commuter Challenge Goals: · Motivate sustainable action on campus · Introduce sustainability concepts using a fun, interactive approach Outcomes: · Improved

  11. Women in management: gender, age and working lives 

    E-Print Network [OSTI]

    Jyrkinen, Marjut; McKie, Linda

    The project reported in this briefing paper explored how age and gender inter-weave and impact on the working and home lives of women managers working in two EU member states, namely Finland and Scotland. The proportion of women in middle...

  12. Safety and Liveness in Intelligent Intersections Hemant Kowshik1

    E-Print Network [OSTI]

    of designing intelligent intersections where traffic lights and stop signs are removed, and cars negotiate intersections are representative of complex distributed hybrid systems which need architectures and algorithms with provable safety and liveness. We propose a hybrid architecture which involves an appropriate inter- play

  13. Distribution of ranks of ?-decay half-lives

    E-Print Network [OSTI]

    Juan Miguel Campanario

    2010-11-21T23:59:59.000Z

    I studied the distribution of ranks of values of 2949 {\\beta}-decay half-lives according to an empirical beta law with two exponents. {\\beta}-decay half-life ranks showed good fit to a beta function with two exponents.

  14. Adaptive Traffic-Based Techniques For Live Multimedia Streaming

    E-Print Network [OSTI]

    Adaptive Traffic-Based Techniques For Live Multimedia Streaming Gabriel-Miro Muntean and LiamMiro.Muntean@ucd.ie, Liam.Murphy@ucd.ie Abstract The growing number of multimedia stream transmissions done through the time-sensitive applications and especially the continuity of multimedia streaming. The paper presents

  15. Live in Spain while earning academic credit Spring 2013

    E-Print Network [OSTI]

    + + + Live in Spain while earning academic credit Spring 2013 · Study at one of Spain's oldest Spain with other CSU students · Take advantage of the opportunities you will have to travel throughout Spain & greater Europe Centrally located 20 miles from the vibrant city of Madrid, Alcalá de Henares

  16. LIVE. WORK. CELEBRATE. Email order to: Beverlyhills@cort.com

    E-Print Network [OSTI]

    Williams, Gary A.

    and Lamp LIVING ROOM: Sofa, Chair, Cocktail Table, End Table, Lamp, plus 32" LCD TV with Stand STUDY: Chair TV with Stand, Accent Pillows and 3 Pieces of Artwork STUDY: Chair and Lamp DINING ROOM: Dining Table drop it off, set it up and pick it up n No heavy lifting or assembly required n Fit a student

  17. ith America focused on energy alternatives and green living,

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    W ith America focused on energy alternatives and green living, Oklahoma State University- Tulsa. His process to produce a clean, alternative energy source could significantly reduce the use of fossil it a very attractive alternative to solar energy technology. "Thermoelectric materials are so much less

  18. http://tti.tamu.edu Saving Lives, Time and Resources

    E-Print Network [OSTI]

    and organization > Project management > Education and training > Costs > Capital investments > Road pricinghttp://tti.tamu.edu Saving Lives, Time and Resources A Member of The Texas A&M University System estimation > Traffic forecasting > Commuting > Carpools > Highway operations > Highway capacity > Freeway

  19. Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE

    E-Print Network [OSTI]

    Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE ENVIRONMENT Environmental Studies 50 steps necessary for this goal. We begin by defining "green building" and explaining its relevance to the College's plans for new residences. Green building incorporates sensitivity towards the environment

  20. Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE

    E-Print Network [OSTI]

    Green Living At Dartmouth College BUILDINGS, COMMUNITY AND THE ENVIRONMENT Environmental Studies 50 Recommendations 3 a. Chapters I and II: Green Building and New Dorms 3 b. Chapter III: Existing Dorms 3 c. Chapter Institutions 5 CHAPTER 2: GREEN BUILDING 6 CHAPTER 3: NEW DORMS 9 I. The Need for New Housing 10 II. Overview

  1. Performance and Energy Modeling for Live Migration of Virtual Machines

    E-Print Network [OSTI]

    Xu, Cheng-Zhong

    Performance and Energy Modeling for Live Migration of Virtual Machines Haikun Liu , Cheng-Zhong Xu , Hai Jin , Jiayu Gong , Xiaofei Liao School of Computer Science and Technology Huazhong University of Science and Technology Wuhan, 430074, China {hjin, xfliao}@hust.edu.cn Department of Electrical

  2. Residential Services Area Missing Students living in University Managed Accommodation

    E-Print Network [OSTI]

    Sussex, University of

    Residential Services Area Missing Students living in University Managed Accommodation 1.0 Where then report to the Building Manager or to the Residential Student Support Team or the Residential Services issues Residential Student Support Manager or the Residential Services Manager should be contacted

  3. Vertebrate heart development: Lessons learnt from live imaging

    E-Print Network [OSTI]

    Shyamasundar, R.K.

    Vertebrate heart development: Lessons learnt from live imaging California Institute of Technology employing different imaging techniques. Sub resolution imaging of beating zebrafish heart has however remained a challenge owing Embryonic heart is a 100 moving quasi-periodically at few Hertz frequency, over

  4. The importance of live biofilms in corrosion protection

    E-Print Network [OSTI]

    Wood, Thomas K.

    Letter The importance of live biofilms in corrosion protection Rongjun Zuo a , Esra Kus b , Florian, University of Connecticut, 191 Auditorium Road, U-3222, Storrs, CT 06269-3222, USA b Corrosion occurred within a few hours as indicated by characteristic changes in the impedance spectra. The corrosion

  5. Healthy Foods, Healthy Lives: Cooking on a Student's Budget

    E-Print Network [OSTI]

    Amin, S. Massoud

    Healthy Foods, Healthy Lives: Cooking on a Student's Budget FScN 2002 ­ Fall & Spring Semesters/monthly menus within a realistic food budget and a modest supply list Gain valuable information and connect to a variety of resources on nutrition, food safety, budgeting and meal planning Develop a social network

  6. Published in Artificial Life V: Proc. Fifth Inter. Conf. on the Synthesis and Simulation of Living Systems, Nara, Japan, May, 1996. Perception and Learning in Artificial Animals

    E-Print Network [OSTI]

    Terzopoulos, Demetri

    Systems, Nara, Japan, May, 1996. Perception and Learning in Artificial Animals Demetri Terzopoulos, Tamer active perception system that enables artificial marine animals to navigate purposefully throughPublished in Artificial Life V: Proc. Fifth Inter. Conf. on the Synthesis and Simulation of Living

  7. Published in Artificial Life V: Proc. Fifth Inter. Conf. on the Synthesis and Simulation of Living Systems, Nara, Japan, May, 1996. Perception and Learning in Artificial Animals

    E-Print Network [OSTI]

    Toronto, University of

    Systems, Nara, Japan, May, 1996. Perception and Learning in Artificial Animals Demetri Terzopoulos, Tamer in the dynamic environment [2]. 1 We present a pro­ totype active perception system that enables artificialPublished in Artificial Life V: Proc. Fifth Inter. Conf. on the Synthesis and Simulation of Living

  8. Fossil identification activity Standard: Comparing fossils to each other or to living organisms reveals features of

    E-Print Network [OSTI]

    . Icaronycteris XI6a, aka" bat" 4. Boney Fish (Knightera) XI2c, (1 piece) 5. Megalodon tooth XIa 6. Dinosaur skin? (teeth? skin? bone? imprint?) 3. What kind of animal is it? (reptile? mammal? invertebrate? fish?) 4. Why

  9. activity-long living wastes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    the appeal of some scientific disciplines. Sparavigna, A C 2012-01-01 7 Municipal solid waste characteristics and management in Allahabad, India Renewable Energy Websites...

  10. Short- and long-lived radionuclide particle size measurements in a uranium mine

    SciTech Connect (OSTI)

    Tu, Keng-Wu; Fisenne, I.M.; Hutter, A.R.

    1997-04-01T23:59:59.000Z

    The radon-222 progeny and long-lived radionuclide measurements were done in a wet underground uranium mine in Saskatchewan, Canada, on Nov. 8-12, 1995. Radon-222 in the mine varied from 2 kBq/m{sup 3} at 90 m below surface to 12 kBq/m{sup 3} in the mining areas, 240 m below surface. Radon-222 progeny activity and potential alpha energy concentration appear affected by the airborne particle number concentration and size distribution. Particle number was up to 200x10{sup 3}/cm{sup 3}. Only an accumulation mode (30-1000 nm) and some bimodal size distributions in this accumulation size range were significant. Diesel particles and combustion particles from burning propane caused a major modal diameter shift to a smaller size range (50-85 nm) compared with previous values (100-200 nm). The high particle number reduced the unattached progeny (0.5-2 nm) to >5%. The nuclei mode (2-30 nm) in this test was nonexistent, and the coarse mode (>1000 nm), except from the drilling areas and on the stopes, was mostly not measurable. Airborne particle total mass and long- lived radionuclide alpha activity concentrations were very low (80- 100 {mu}g/m{sup 3} and 4-5 mBq/m{sup 3}) owing to high ventilation rates. Mass-weighted size distributions were trimodal, with the major mode at the accumulation size region, which accounts for 45-50% of the mass. The coarse model contains the the least mass, about 20%. The size spectra from gross alpha activities were bimodal with major mode in the coarse region (>1000 nm) and a minor accumulation mode in the 50-900 nm size range. These size spectra were different from the {sup 222}Rn progeny that showed a single accumulation mode in the 50- 85 nm size region. The accumulation mode in the long-lived radionuclide size spectrum was not found in previous studies in other uranium mines.

  11. activity policy environments: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    activity policy environments First Page Previous Page 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Next Page Last Page Topic Index 1 Connecting Active Living...

  12. Phospholamban mutants compete with wild type for SERCA binding in living cells

    SciTech Connect (OSTI)

    Gruber, Simon J.; Haydon, Suzanne [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)] [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States); Thomas, David D., E-mail: ddt@umn.edu [Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455 (United States)

    2012-04-06T23:59:59.000Z

    Highlights: Black-Right-Pointing-Pointer PLB phosphorylation in HEK cells increased FRET between YFP-PLB and CFP-SERCA. Black-Right-Pointing-Pointer Competition: Expressing loss-of-function PLB mutants in the system decreased FRET. Black-Right-Pointing-Pointer The FRET assay could screen potential therapeutic PLB mutants to activate SERCA. -- Abstract: We have used fluorescent fusion proteins stably expressed in HEK cells to detect directly the interaction between the sarcoplasmic reticulum Ca-ATPase (SERCA) and phospholamban (PLB) in living cells, in order to design PLB mutants for gene therapy. Ca{sup 2+} cycling in muscle cells depends strongly on SERCA. Heart failure (HF), which contributes to 12% of US deaths, typically exhibits decreased SERCA activity, and several potential therapies for HF aim to increase SERCA activity. We are investigating the use of LOF-PLB mutants (PLB{sub M}) as gene therapy vectors to increase SERCA activity. Active SERCA1a and WT-PLB, tagged at their N termini with fluorescent proteins (CFP and YFP), were coexpressed in stable HEK cell lines, and fluorescence resonance energy transfer (FRET) was used to detect their interaction directly. Phosphorylation of PLB, induced by forskolin, caused an increase in FRET from CFP-SERCA to YFP-PLB, indicating that SERCA inhibition can be relieved without dissociation of the complex. This suggests that a LOF mutant might bind to SERCA with sufficient affinity to complete effectively with WT-PLB, thus relieving SERCA inhibition. Therefore, we transiently expressed a series of PLB{sub M} in the CFP-SERCA/YFP-PLB cell line, and found decreased FRET, implying competition between PLB{sub M} and WT-PLB for binding to SERCA. These results establish this FRET assay as a rapid and quantitative means of screening PLB{sub M} for optimization of gene therapy to activate SERCA, as needed for gene therapy in HF.

  13. Axion cosmology with long-lived domain walls

    SciTech Connect (OSTI)

    Hiramatsu, Takashi [Yukawa Institute for Theoretical Physics, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502 (Japan); Kawasaki, Masahiro; Saikawa, Ken'ichi [Institute for Cosmic Ray Research, The University of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa City, Chiba 277-8582 (Japan); Sekiguchi, Toyokazu, E-mail: hiramatz@yukawa.kyoto-u.ac.jp, E-mail: kawasaki@icrr.u-tokyo.ac.jp, E-mail: saikawa@icrr.u-tokyo.ac.jp, E-mail: sekiguti@a.phys.nagoya-u.ac.jp [Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya City, Aichi 464-8602 (Japan)

    2013-01-01T23:59:59.000Z

    We investigate the cosmological constraints on axion models where the domain wall number is greater than one. In these models, multiple domain walls attached to strings are formed, and they survive for a long time. Their annihilation occurs due to the effects of explicit symmetry breaking term which might be raised by Planck-scale physics. We perform three-dimensional lattice simulations and compute the spectra of axions and gravitational waves produced by long-lived domain walls. Using the numerical results, we estimated relic density of axions and gravitational waves. We find that the existence of long-lived domain walls leads to the overproduction of cold dark matter axions, while the density of gravitational waves is too small to observe at the present time. Combining the results with other observational constraints, we find that the whole parameter region of models are excluded unless an unacceptable fine-tuning exists.

  14. NUCLEAR HALF-LIVES FOR ALPHA RADIOACTIVITY OF ELEMENTS WITH

    E-Print Network [OSTI]

    P. Roy Chowdhury A; C. Samanta A

    Theoretical estimates for the half lives of about 1700 isotopes of heavy elements with 100 ? Z ? 130 are tabulated using theoretical Q-values. The quantum mechanical tunneling probabilities are calculated within a WKB framework using microscopic nuclear potentials. The microscopic nucleus- nucleus potentials are obtained by folding the densities of interacting nuclei with a density dependent M3Y (DDM3Y) effective nucleon- nucleon interaction. The ?-decay half lives calculated in this formalism using the experimental Q-values were found to be in good agreement over a wide range of experimental data spanning about twenty orders of magnitude. The theoretical Q-values used for the present calculations are extracted from three different mass estimates viz. Myers-Swiatecki [MS], Muntian-Hofmann-Patyk-

  15. The problem of living in a world contaminated with chemicals

    SciTech Connect (OSTI)

    Metcalf, R.L. [Univ. of Illinois, Urbana (United States)

    1990-12-31T23:59:59.000Z

    The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

  16. Single-photon ultrashort-lived radionuclides: symposium proceedings

    SciTech Connect (OSTI)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01T23:59:59.000Z

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  17. Dynamic self-assembly in living systems as computation.

    SciTech Connect (OSTI)

    Bouchard, Ann Marie; Osbourn, Gordon Cecil

    2004-06-01T23:59:59.000Z

    Biochemical reactions taking place in living systems that map different inputs to specific outputs are intuitively recognized as performing information processing. Conventional wisdom distinguishes such proteins, whose primary function is to transfer and process information, from proteins that perform the vast majority of the construction, maintenance, and actuation tasks of the cell (assembling and disassembling macromolecular structures, producing movement, and synthesizing and degrading molecules). In this paper, we examine the computing capabilities of biological processes in the context of the formal model of computing known as the random access machine (RAM) [Dewdney AK (1993) The New Turing Omnibus. Computer Science Press, New York], which is equivalent to a Turing machine [Minsky ML (1967) Computation: Finite and Infinite Machines. Prentice-Hall, Englewood Cliffs, NJ]. When viewed from the RAM perspective, we observe that many of these dynamic self-assembly processes - synthesis, degradation, assembly, movement - do carry out computational operations. We also show that the same computing model is applicable at other hierarchical levels of biological systems (e.g., cellular or organism networks as well as molecular networks). We present stochastic simulations of idealized protein networks designed explicitly to carry out a numeric calculation. We explore the reliability of such computations and discuss error-correction strategies (algorithms) employed by living systems. Finally, we discuss some real examples of dynamic self-assembly processes that occur in living systems, and describe the RAM computer programs they implement. Thus, by viewing the processes of living systems from the RAM perspective, a far greater fraction of these processes can be understood as computing than has been previously recognized.

  18. ClimateChangeLIVE Webcast: Join the Climate Conversation

    Broader source: Energy.gov [DOE]

    Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

  19. Half-lives of Double $?^+$-decay with Two Neutrinos

    E-Print Network [OSTI]

    Yuejiao Ren; Zhongzhou Ren

    2015-01-07T23:59:59.000Z

    Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated half-lives of double $\\beta ^+$-decay in this article will be useful for future experimental search of double $\\beta ^+$-decay.

  20. Mass Spectral Molecular Networking of Living Microbial Colonies

    SciTech Connect (OSTI)

    Watrous, Jeramie D.; Roach, Patrick J.; Alexandrov, Theodore; Heath, Brandi S.; Yang, Jane Y.; Kersten, Roland; vander Voort, Menno; Pogliano, Kit; Gross, Harald; Raaijmakers, Jos M.; Moore, Bradley S.; Laskin, Julia; Bandeira, Nuno; Dorrestein, Pieter C.

    2012-06-26T23:59:59.000Z

    Integrating the governing chemistry with the genomics and phenotypes of microbial colonies has been a "holy grail" in microbiology. This work describes a highly sensitive, broadly applicable, and costeffective approach that allows metabolic profiling of live microbial colonies directly from a Petri dish without any sample preparation. Nanospray desorption electrospray ionization mass spectrometry (MS), combined with alignment of MS data and molecular networking, enabled monitoring of metabolite production from live microbial colonies from diverse bacterial genera, including Bacillus subtilis, Streptomyces coelicolor, Mycobacterium smegmatis, and Pseudomonas aeruginosa. This work demonstrates that, by using these tools to visualize small molecular changes within bacterial interactions, insights can be gained into bacterial developmental processes as a result of the improved organization of MS/MS data. To validate this experimental platform, metabolic profiling was performed on Pseudomonas sp. SH-C52, which protects sugar beet plants from infections by specific soil-borne fungi [R. Mendes et al. (2011) Science 332:1097–1100]. The antifungal effect of strain SHC52 was attributed to thanamycin, a predicted lipopeptide encoded by a nonribosomal peptide synthetase gene cluster. Our technology, in combination with our recently developed peptidogenomics strategy, enabled the detection and partial characterization of thanamycin and showed that it is amonochlorinated lipopeptide that belongs to the syringomycin family of antifungal agents. In conclusion, the platform presented here provides a significant advancement in our ability to understand the spatiotemporal dynamics of metabolite production in live microbial colonies and communities.

  1. Arsenic exposure in children living near a former copper smelter

    SciTech Connect (OSTI)

    Binder, S.; Forney, D.; Kaye, W.; Paschal, D.

    1987-07-01T23:59:59.000Z

    About 10,000 people live in communities surrounding the former copper smelter at Anaconda, Montana. Most of these people live in the town of Anaconda, which is generally upwind of the smelter. The smelter ceased operations in 1980, after almost a century of ore processing. Soil and dust on the smelter site and in the vicinity remain contaminated with arsenic, although at this time air and drinking water arsenic levels are not elevated. Results of soil and dust sampling for arsenic in the communities around the smelter are reported. In the town of Anaconda, surface soil arsenic levels from residential sites have averaged around 100 ppm or greater. Young children are generally believed to be the population with the most nonoccupational exposure to soil. Several models of exposure to environmental arsenic in the Anaconda area have predicted that children living in all communities surrounding the smelter would be having significant and measurable exposure to arsenic. Two exposures surveys, conducted while the smelter was operative, demonstrated that excess exposure to arsenic was occurring in young children. Until the present surveys, no exposure data had been collected since the smelter was closed.

  2. Production of Crosslinked, Hollow Nanoparticles by Surface-Initiated Living Free-Radical Polymerization

    E-Print Network [OSTI]

    Harth, Eva M.

    Production of Crosslinked, Hollow Nanoparticles by Surface-Initiated Living Free alkoxyamine initiating groups are attached to the surface silanol groups of silica nanoparticles. This surface Keywords: living radical; nitroxide; nanoparticles; crosslinking; core­shell; poly- mers; nanotechnology

  3. Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow...

    Broader source: Energy.gov (indexed) [DOE]

    Summit Live: 9:30-10 AM ET Today and All Day Tomorrow Tribal Summit Live: 9:30-10 AM ET Today and All Day Tomorrow May 4, 2011 - 8:00am Addthis Ginny Simmons Ginny Simmons Former...

  4. How do Communities Change their Culture Towards more Sustainable Patterns of Living, Working and Learning?

    E-Print Network [OSTI]

    How do Communities Change their Culture Towards more Sustainable Patterns of Living, Working towards more sustainable ways of living; a case study of the Sustainability Office's efforts to change Staff: Heather Scholefield, Strategy Manager, Sustainability Office, Land and Building Services

  5. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

    2009-04-28T23:59:59.000Z

    Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  6. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir, Sanjiv (Portola Valley, CA); Pritha, Ray (Mountain View, CA)

    2011-06-07T23:59:59.000Z

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  7. Live Imaging Study on Cytokinin Function and Regulation in Stem-cell Homeostasis

    E-Print Network [OSTI]

    Xie, Mingtang

    2010-01-01T23:59:59.000Z

    on research on SAM Live-image technology was developed toin SAMs, transient perturbations, live image and cell typetreated. (C) The images from the same SAM were used to score

  8. Watch Live: National Science Bowl - Starting At 9:30 AM ET |...

    Energy Savers [EERE]

    Watch Live: National Science Bowl - Starting At 9:30 AM ET Watch Live: National Science Bowl - Starting At 9:30 AM ET May 2, 2011 - 8:55am Addthis Ginny Simmons Ginny Simmons...

  9. X-ray Computed Tomography of the Root System of a Live Potted...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    X-ray Computed Tomography of the Root System of a Live Potted Plant X-ray Computed Tomography of the Root System of a Live Potted Plant Being able to see the root structure of a...

  10. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

    Office of Environmental Management (EM)

    TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

  11. Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent longevity

    E-Print Network [OSTI]

    Huchon, Dorothée

    Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent 31905, Israel Summary Naked mole-rats (Heterocephalus glaber), the longest-lived rodents, live 7% of their lives. Little is known about the mechanisms that allow them to delay the aging process and live so long

  12. Ameliorating risk: Culturable and metagenomic monitoring of the 14 year decline of a genetically engineered microorganism at a bioremediation field site

    SciTech Connect (OSTI)

    Layton, Alice [University of Tennessee, Knoxville (UTK); Smart, Abby E. [University of Tennessee, Knoxville (UTK); Chauhan, Archana [University of Tennessee, Knoxville (UTK); Ripp, Steven Anthony [University of Tennessee, Knoxville (UTK); Williams, Daniel [University of Tennessee, Knoxville (UTK); Burton, Whitney [University of Tennessee, Knoxville (UTK); Moser, Scott [University of Tennessee, Knoxville (UTK); Phillips, Jana Randolph [ORNL; Palumbo, Anthony Vito [ORNL; Sayler, Gary [University of Tennessee, Knoxville (UTK)

    2012-01-01T23:59:59.000Z

    Pseudomonas fluorescens HK44 represented the first genetically engineered microorganism to be approved in the United States for field release for applications related to subsurface soil bioremediation. In October 1996, strain HK44 was introduced into a replicated semi-contained array of soil lysimeters where its luciferase (luxCDABE)-based bioluminescent response to soil-borne polycyclic aromatic hydrocarbon (PAH) contaminants was detected and monitored for the next two years. At the termination of this experiment, it was decided that the lysimeters remain available for future longer-term monitoring efforts, and were thus covered and left essentially undisturbed until the initiation of a large sampling event in 2010, fourteen years after the original release. Although after extensive sampling culturable HK44 cells were not found, additional molecular and metagenomic analyses indicated that genetic signatures of HK44 cells still persisted, with genes diagnostic for the bioluminescent transposon carried by strain HK44 (luxA and tetA) being found at low concentrations (< 5000 copies/g).

  13. Experimental test of whether electrostatically charged micro-organisms and their spores contribute to the onset of arcs across vacuum gaps

    SciTech Connect (OSTI)

    Grisham, L. R.; Halle, A. von; Carpe, A. F.; Gilton, K. R.; Rossi, Guy; Stevenson, T. N. [Princeton University Plasma Physics Laboratory, P. O. Box 451, Princeton, New Jersey 08543 (United States)] [Princeton University Plasma Physics Laboratory, P. O. Box 451, Princeton, New Jersey 08543 (United States)

    2013-12-15T23:59:59.000Z

    Recently it was proposed [L. R. Grisham et al. Phys. Plasmas 19, 023107 (2012)] that one of the initiators of vacuum voltage breakdown between conducting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which then become electrostatically charged when an electric potential is applied across the vacuum gap. This note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maximum operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each case preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance.

  14. Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps

    SciTech Connect (OSTI)

    none,; Grisham, Larry R.

    2014-02-24T23:59:59.000Z

    Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

  15. HOUSING/LIVING ARRANGEMENT CHANGE DO NOT USE THIS FORM TO CHANGE YOUR ADDRESS.

    E-Print Network [OSTI]

    Amin, S. Massoud

    HOUSING/LIVING ARRANGEMENT CHANGE DO NOT USE THIS FORM TO CHANGE YOUR ADDRESS. Go online to onestop, select the appropriate housing option. If you live in off-campus housing (without parents), you must have, state, ZIP code, country) PART 2. Housing Information Please change my housing/living arrangements

  16. Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing Housing and Residential Life is proud to offer many Living Learning Communities (LLC) and special interest housing on campus. These communities provide a great opportunity to live with others who share your

  17. Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    Housing and Residential Life Rate Sheet Living Learning Communities & Special Interest Housing Housing and Residential Life is proud to offer many Living-Learning Communities (LLC) and special interest housing on campus. These communities provide a great opportunity to live with others who share your

  18. Reproductive Life Events in the Population Living in the Vicinity of a Nuclear Waste Reprocessing Plant

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    Reproductive Life Events in the Population Living in the Vicinity of a Nuclear Waste Reprocessing: There is concern about the health of populations living close to nuclear waste reprocessing plants. We conducted a comparative study on reproductive life events in the general population living near the nuclear waste

  19. The Living Site and Infrastructure Challenge In pursuit of true sustainability in the built environment

    E-Print Network [OSTI]

    Zaferatos, Nicholas C.

    The Living Site and Infrastructure Challenge In pursuit of true sustainability in the built environment Draft Version 1.0 November 2007 #12;NOTIFICATION The Living Site and Infrastructure Challenge - copyright 2007 - Cascadia Region Green Building Council, all rights reserved. The Living Site

  20. Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University

    E-Print Network [OSTI]

    Bertini, Robert L.

    Intelligent Transportation Systems: Saving Lives, Time and Money Portland State University Oct 14, 2009 #12;Intelligent Transportation Systems: Saving Lives, Time and Money James Whiteneck #12;Intelligent Transportation Systems: Saving Lives, Time and Money Portal archives a large amount of data Over

  1. 1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee

    E-Print Network [OSTI]

    Bertini, Robert L.

    1Intelligent Transportation Systems: Saving Lives, Time and Money PORTAL Advisory Committee Initial Meeting January 22, 2009 #12;2Intelligent Transportation Systems: Saving Lives, Time and Money Agenda 9:00 Adjourn #12;3Intelligent Transportation Systems: Saving Lives, Time and Money What's in the PORTAL

  2. Mitigating container security risk using real-time monitoring with active Radio Frequency Identification and sensors

    E-Print Network [OSTI]

    Schlesinger, Adam Ian

    2005-01-01T23:59:59.000Z

    The global village in which we live enables increased trade and commerce across regions but also brings a complicated new set of challenges such as terrorist activity, human and drug smuggling and theft in foreign or ...

  3. Comparison of median frequency between traditional and functional sensor placements during activity monitoring

    E-Print Network [OSTI]

    Graham, Selina

    Long-term monitoring is of great clinical relevance. Accelerometers are often used to provide information about activities of daily living. The median frequency (f[subscript m]) of acceleration has recently been suggested ...

  4. Production of short lived radioactive beams of radium

    E-Print Network [OSTI]

    Shidling, P D; van der Hoek, D J; Jungmann, K; Kruithof, W; Onderwater, C J G; Sohani, M; Versolato, O O; Willmann, L; Wilschut, H W

    2009-01-01T23:59:59.000Z

    Short lived $^{212,213,214}$Ra isotopes have been produced at the TRI$\\mu$P facility in inverse kinematics via the fusion-evaporation reaction $^{206}$Pb+$^{12}$C at 8 MeV/u. Isotopes are separated from other reaction products online using the TRI$\\mu$P magnetic separator. The energetic radium (Ra) isotopes at the exit of the separator were converted into low energy ions with a thermal ionizer. Ra isotopes have been identified by observing their $\\alpha$ decay and life times.

  5. Production of short lived radioactive beams of radium

    E-Print Network [OSTI]

    P. D. Shidling; G. S. Giri; D. J. van der Hoek; K. Jungmann; W. Kruithof; C. J. G. Onderwater; M. Sohani; O. O. Versolato; L. Willmann; H. W. Wilschut

    2009-02-25T23:59:59.000Z

    Short lived $^{212,213,214}$Ra isotopes have been produced at the TRI$\\mu$P facility in inverse kinematics via the fusion-evaporation reaction $^{206}$Pb+$^{12}$C at 8 MeV/u. Isotopes are separated from other reaction products online using the TRI$\\mu$P magnetic separator. The energetic radium (Ra) isotopes at the exit of the separator were converted into low energy ions with a thermal ionizer. Ra isotopes have been identified by observing their $\\alpha$ decay and life times.

  6. Probing the membrane potential of living cells by dielectric spectroscopy

    E-Print Network [OSTI]

    Corina Bot; Camelia Prodan

    2008-12-17T23:59:59.000Z

    In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtain from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli were chosen for our experiments.

  7. Living well, doing good | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch > The EnergyCenter (LMI-EFRC) - CenterLinks BerkeleyLiving well, doing

  8. Live Oak County, Texas: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov YouKizildere I Geothermal Pwer Plant Jump to:Landowners and WindLightingLinthicum,Little ValleyLiuzhou Xinneng BiomassLive

  9. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron Spin Transition in2, 2003Tool ofTopo II:7.1Tracking Living Cells as They

  10. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level:Energy: Grid Integration Redefining What'sis Taking Over OurThe Iron Spin Transition in2, 2003Tool ofTopo II:7.1Tracking Living Cells as

  11. How Will We Live Forever? | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC) EnvironmentalGyroSolé(tm) HarmonicbetandEnergy 2010 ABringofExplore Earth'sLive

  12. Live Discussion on Energy 101: Fuel Cells | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -ChoicesListLive Discussion on

  13. Live webcast on groundbreaking results of Algal Biofuels Consortium, June

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of Science (SC)Integrated Codes |Is Your HomeLatestCenter (LMI-EFRC) -ChoicesListLive

  14. JV between KP Renewables and Living Buildings | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page onYou are now leaving Energy.gov You are now leaving Energy.gov You are8COaBulkTransmissionSitingProcess.pdfGetecGtelInterias Solar EnergyEnergy Information Bar LMorgan JumpLiving

  15. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » TopTours SignTracking Living Cells

  16. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645U.S. DOE Office of ScienceandMesa del SolStrengthening a solidSynthesisAppliances » TopTours SignTracking Living

  17. Towards Plasma Surgery: Plasma Treatment of Living Cells

    SciTech Connect (OSTI)

    Stoffels, E.; Kieft, I.E.; Sladek, R.E.J.; Slaaf, D.W.; Laan, E.P. van der; Jimenez-Moreno, P.; Steinbuch, M. [Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven (Netherlands); Department of Mechanical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB, Eindhoven (Netherlands)

    2004-12-01T23:59:59.000Z

    The physical, biological and technical background for high-precision plasma surgery is prepared in a multi-disciplinary team. The aim of the research is to achieve controlled removal of diseased cells and bacteria without harming the healthy rest of the tissue. For this purpose, a small, cold, flexible and non-toxic plasma is developed (the plasma needle) and tested on cultured cells and bacterial samples. The needle is an atmospheric discharge induced by a radio-frequency voltage applied to a metal pin. This plasma operates at room temperature, in the milliwatt power regime; it poses no risk of thermal or electrical damage to living tissues. Several beneficial responses of living cells to plasma treatment have been already identified. Plasma does not cause accidental cell death (necrosis), which leads to inflammation and tissue damage. Instead, it allows to detach cells from each other and from the scaffold, and thus to remove them in a non-destructive way. Moreover, plasma is capable of bacterial inactivation. I parallel, we have determined the electrical and optical properties of the plasma and found a method of precise positioning of the plasma needle with respect to the treated tissue.

  18. Report on First Activations with the Lead Slowing Down Spectrometer

    SciTech Connect (OSTI)

    Warren, Glen A.; Mace, Emily K.; Pratt, Sharon L.; Stave, Sean; Woodring, Mitchell L.

    2011-03-03T23:59:59.000Z

    On Feb. 17 and 18 2011, six items were irradiated with neutrons using the Lead Slowing Down Spectrometer. After irradiation, dose measurements and gamma-spectrometry measurements were completed on all of the samples. No contamination was found on the samples, and all but one provided no dose. Gamma-spectroscopy measurements qualitatively agreed with expectations based on the materials, with the exception of silver. We observed activation in the room in general, mostly due to 56Mn and 24Na. Most of the activation was short lived, with half-lives on the scale of hours, except for 198Au which has a half-life of 2.7 d.

  19. "Each and everyone of us can make changes in the way we live our lives and become part of the solution [to climate change]"

    E-Print Network [OSTI]

    Scharfstein, Daniel

    Green Guide "Id put my money on the sun and solar energy. What a source of power! I hope we dont have of the solution [to climate change]" Al Gore, ,,An Inconvenient Truth BaltimoreGreen Living Guide www.jhsph.edu/green content useful. Cheers, and happy green living! "Be the change you want to see in the world" -- Gandhi

  20. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-01-01T23:59:59.000Z

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  1. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-30T23:59:59.000Z

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  2. Magnetic resonance imaging of living systems by remote detection

    DOE Patents [OSTI]

    Wemmer, David; Pines, Alexander; Bouchard, Louis; Xu, Shoujun; Harel, Elad; Budker, Dmitry; Lowery, Thomas; Ledbetter, Micah

    2013-10-29T23:59:59.000Z

    A novel approach to magnetic resonance imaging is disclosed. Blood flowing through a living system is prepolarized, and then encoded. The polarization can be achieved using permanent or superconducting magnets. The polarization may be carried out upstream of the region to be encoded or at the place of encoding. In the case of an MRI of a brain, polarization of flowing blood can be effected by placing a magnet over a section of the body such as the heart upstream of the head. Alternatively, polarization and encoding can be effected at the same location. Detection occurs at a remote location, using a separate detection device such as an optical atomic magnetometer, or an inductive Faraday coil. The detector may be placed on the surface of the skin next to a blood vessel such as a jugular vein carrying blood away from the encoded region.

  3. Near-Term Climate Mitigation by Short-Lived Forcers

    SciTech Connect (OSTI)

    Smith, Steven J.; Mizrahi, Andrew H.

    2013-08-12T23:59:59.000Z

    Emissions reductions focused on anthropogenic climate forcing agents with relatively short atmospheric lifetimes such as methane (CH4) and black carbon (BC) have been suggested as a strategy to reduce the rate of climate change over the next several decades. We find that reductions of methane and BC would likely have only a modest impact on near-term climate warming. Even with maximally feasible reductions phased in from 2015 to 2035, global mean temperatures in 2050 are reduced by 0.16 °C, with an uncertainty range of 0.04-0.36°C, with the high end of this range only possible if total historical aerosol forcing is small. More realistic mitigation scenarios would likely provide a smaller climate benefit. The climate benefits from targeted reductions in short-lived forcing agents are smaller than previously estimated and are not substantially different in magnitude from the benefits due to a comprehensive climate policy.

  4. Nonequilibrium equation of state in suspensions of active colloids

    E-Print Network [OSTI]

    Félix Ginot; Isaac Theurkauff; Demian Levis; Christophe Ybert; Lydéric Bocquet; Ludovic Berthier; Cécile Cottin-Bizonne

    2014-11-26T23:59:59.000Z

    Active colloids constitute a novel class of materials composed of colloidal-scale particles locally converting chemical energy into motility, mimicking micro-organisms. Evolving far from equilibrium, these systems display structural organizations and dynamical properties distinct from thermalized colloidal assemblies. Harvesting the potential of this new class of systems requires the development of a conceptual framework to describe these intrinsically nonequilibrium systems. We use sedimentation experiments to probe the nonequilibrium equation of state of a bidimensional assembly of active Janus microspheres, and conduct computer simulations of a model of self-propelled hard disks. Self-propulsion profoundly affects the equation of state, but these changes can be rationalized using equilibrium concepts. We show that active colloids behave, in the dilute limit, as an ideal gas with an activity-dependent effective temperature. At finite density, increasing the activity is similar to increasing adhesion between equilibrium particles. We quantify this effective adhesion and obtain a unique scaling law relating activity and effective adhesion in both experiments and simulations. Our results provide a new and efficient way to understand the emergence of novel phases of matter in active colloidal suspensions.

  5. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, A.

    2014-09-01T23:59:59.000Z

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  6. Our academic landscape is rapidly changing. We all live in a digital world. Use of technology infuses every part of our personal and professional lives and our connections are global. Technology has brought us new and

    E-Print Network [OSTI]

    Haase, Markus

    infuses every part of our personal and professional lives and our connections are global. Technology has

  7. Human Activity Detection from RGBD Images

    E-Print Network [OSTI]

    Sung, Jaeyong; Selman, Bart; Saxena, Ashutosh

    2011-01-01T23:59:59.000Z

    Being able to detect and recognize human activities is important for making personal assistant robots useful in performing assistive tasks. The challenge is to develop a system that is low-cost, reliable in unstructured home settings, and also straightforward to use. In this paper, we use a RGBD sensor (Microsoft Kinect) as the input sensor, and present learning algorithms to infer the activities. Our algorithm is based on a hierarchical maximum entropy Markov model (MEMM). It considers a person's activity as composed of a set of sub-activities, and infers the two-layered graph structure using a dynamic programming approach. We test our algorithm on detecting and recognizing twelve different activities performed by four people in different environments, such as a kitchen, a living room, an office, etc., and achieve an average performance of 84.3% when the person was seen before in the training set (and 64.2% when the person was not seen before).

  8. Towards a 'Thermodynamics' of Active Matter

    E-Print Network [OSTI]

    Sho C. Takatori; John F. Brady

    2014-11-21T23:59:59.000Z

    Self-propulsion allows living systems to display unusual collective behavior. Unlike passive systems in thermal equilibrium, active matter systems are not constrained by conventional thermodynamic laws. A question arises however as to what extent, if any, can concepts from classical thermodynamics be applied to nonequilibrium systems like active matter. Here we use the new swim pressure perspective to develop a simple theory for predicting phase separation in active matter. Using purely mechanical arguments we generate a phase diagram with a spinodal and critical point, and define a nonequilibrium chemical potential to interpret the "binodal." We provide a generalization of thermodynamic concepts like the free energy and temperature for nonequilibrium active systems. Our theory agrees with existing simulation data both qualitatively and quantitatively and may provide a framework for understanding and predicting the behavior of nonequilibrium active systems.

  9. The effects of wavelength, metals, and reactive oxygen species on the sunlight inactivation of microorganisms: observations and applications to the solar disinfection of drinking water

    E-Print Network [OSTI]

    Fisher, Michael Benjamin

    2011-01-01T23:59:59.000Z

    Ultraviolet-A-Irradiated Riboflavin Solution Is Derived fromand Pfeifer, G.P. (2007) Riboflavin activated by ultravioletColi Mutants Deficient in Riboflavin Biosynthesis with

  10. Sunset magazine : in search of a house for western living

    E-Print Network [OSTI]

    Gonzalez, Robert Alexander

    1993-01-01T23:59:59.000Z

    This thesis inquires into Sunset's activity as a home magazine in the 1930s and 1940s. In viewing this period, this study draws from Sunset's entire history: from its inception in 1898 as a travel brochure for Southern ...

  11. Live Webinar on Better Buildings Case Competition: Energy Efficiency in the Restaurant Franchise Model Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  12. Live Webinar on Better Buildings Case Competition: Taking Commercial PACE Financing to Scale, a Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  13. Nuclear $\\beta$-decay half-lives in the relativistic point-coupling model

    E-Print Network [OSTI]

    Wang, Z Y; Niu, Y F; Guo, J Y

    2015-01-01T23:59:59.000Z

    The self-consistent proton-neutron quasiparticle random phase approximation approach is employed to calculate $\\beta$-decay half-lives of neutron-rich even-even nuclei with $8\\leqslant Z \\leqslant 30$. A newly proposed nonlinear point-coupling effective interaction PC-PK1 is used in the calculations. It is found that the isoscalar proton-neutron pairing interaction can significantly reduce $\\beta$-decay half-lives. With an isospin-dependent isoscalar proton-neutron pairing strength, our results well reproduce the experimental $\\beta$-decay half-lives, although the pairing strength is not adjusted using the half-lives calculated in this study.

  14. Vanderbilt University Pet Policy for Live-in Employees Vanderbilt University permits live-in employees, be they faculty or staff, to own and keep pets

    E-Print Network [OSTI]

    Bordenstein, Seth

    1 Vanderbilt University Pet Policy for Live-in Employees Vanderbilt pets in their University assigned housing. The requirements of the policy are as follows: 1. Pets are limited to domesticated dogs, domesticated cats and fish

  15. Memory Saves Lives: Inter-generational Warnings Effectiveness - 13556

    SciTech Connect (OSTI)

    Van Luik, Abraham; Patterson, Russell [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States); Shafer, David [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States)] [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States); Klein, Thomas [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)

    2013-07-01T23:59:59.000Z

    The 2011 Tohoku earthquake and tsunami was a world-class natural disaster. It has been described as the most powerful earthquake ever in Japan, and as one of the most powerful earthquakes ever noted in the world. The toll in terms of human lives lost and property destruction was unimaginable. Even the word 'horrible' is inadequate to describe the suffering and misery that resulted. Nations with nuclear power programs are engaged in, or at least planning to become engaged in, arranging to eventually dispose of their higher-level radioactive waste materials in deep geologic repositories. Geologic repositories are passive safety systems, and if undisturbed isolate these dangerous materials form the biosphere for extremely long times. The key words, however, are 'if undisturbed'. To assure that future generations do not inadvertently drill into repositories, national programs, and the international community (the Records, Knowledge and Memory (RK and M) preservation project of the Nuclear Energy Agency, for example), are proposing to place markers and/or monuments on closed repository sites that say 'do not drill here, and this is why' in various sophisticated ways. Such markers or monuments are attempts at providing passive institutional controls. The effectiveness of messages from past generations to a present generation may give an indication of how effective such passive institutional controls may be. (authors)

  16. Forrestal Building Lighting Retrofit Second Live Test Demonstration (LTD)

    SciTech Connect (OSTI)

    Halverson, M.A.; Schmelzer, J.R.; Parker, G.B.

    1993-02-01T23:59:59.000Z

    This report describes and summarizes the Forrestal Building Lighting Retrofit Live Test demonstration (LTD) performed by Pacific Northwest Laboratory (PNL) in Room 5E-080 of the DOE Forrestal Building in Washington, D.C. The purpose of the LTD was to evaluate proposed lighting retrofits for compliance with the requirements laid out in the request for proposal (RFP) for the Shared Energy Savings (SES) Lighting Retrofit Project for the Forrestal Building, Washington, D.C. Testing was conducted from March 9 through March 18, 1992, and again on August 3 through August 6, 1992. Four contractors were initially tested in March. Then, two contractors were retested in August due to changes in the rebate schedule for electronic ballasts being offered by the Potomac Electric Power Company (PEPCO), the utility servicing the Forrestal Building. The two contractors tested in March were retested with different ballasts, tubes, and reflectors. The results from these new tests are reported here and compared with those from the earlier tests.

  17. Chemical behavior of long-lived radionuclides in the marine environment

    SciTech Connect (OSTI)

    Edgington, D.N.; Nelson, D.M.

    1983-01-01T23:59:59.000Z

    Measurements of long-lived radionuclides in the marine environment have provided a wealth of information regarding the physical, biological, and chemical processes which control the behavior of these and many other pollutants in the oceans. Their value as tracers for the dispersion, transport, and fate of pollutants in the oceans is largely dependent on the chemical properties of each individual radioelement. Differences in these properties, particularly in relation to their interaction with biotic or abiotic particulate matter, result in the separation of parent-daughter radioisotopes in the natural radioelement series or in changes in the ratios of fission and activation products. Such differences have provided the means to provide time scales for a variey of transport processes and to determine sedimentation rates. The properties of these radionuclides in the oceans can, in general, be predicted from the chemical properties of the stable elements. For those elements such as plutonium, for which there are no naturally-occurring stable isotopes, studies of their distribution in the oceans have provided a new important understanding of their chemical behavior. This behavior has not always agreed with what would have been predicted from laboratory studies carried out at far higher concentrations. Differences between observed distributions and laboratory predictions have highlighted the importance of correct experimental conditions in order to avoid confusing experimental artifacts. The interaction of radionuclides with particles in the oceans and marine sediments can be described in terms of simple ion exchange or adsorption equilibria.

  18. Dissecting Regional Variations in Stress Fiber Mechanics in Living Cells with Laser Nanosurgery

    SciTech Connect (OSTI)

    Tanner, Kandice; Boudreau, Aaron; Bissell, Mina J; Kumar, Sanjay

    2010-03-02T23:59:59.000Z

    The ability of a cell to distribute contractile stresses across the extracellular matrix in a spatially heterogeneous fashion underlies many cellular behaviors, including motility and tissue assembly. Here we investigate the biophysical basis of this phenomenon by using femtosecond laser nanosurgery to measure the viscoelastic recoil and cell-shape contributions of contractile stress fibers (SFs) located in specific compartments of living cells. Upon photodisruption and recoil, myosin light chain kinase-dependent SFs located along the cell periphery display much lower effective elasticities and higher plateau retraction distances than Rho-associated kinase-dependent SFs located in the cell center, with severing of peripheral fibers uniquely triggering a dramatic contraction of the entire cell within minutes of fiber irradiation. Image correlation spectroscopy reveals that when one population of SFs is pharmacologically dissipated, actin density flows toward the other population. Furthermore, dissipation of peripheral fibers reduces the elasticity and increases the plateau retraction distance of central fibers, and severing central fibers under these conditions triggers cellular contraction. Together, these findings show that SFs regulated by different myosin activators exhibit different mechanical properties and cell shape contributions. They also suggest that some fibers can absorb components and assume mechanical roles of other fibers to stabilize cell shape.

  19. Identifying Activity

    E-Print Network [OSTI]

    Lewis, Adrian S

    2009-01-01T23:59:59.000Z

    Identification of active constraints in constrained optimization is of interest from both practical and theoretical viewpoints, as it holds the promise of reducing an inequality-constrained problem to an equality-constrained problem, in a neighborhood of a solution. We study this issue in the more general setting of composite nonsmooth minimization, in which the objective is a composition of a smooth vector function c with a lower semicontinuous function h, typically nonsmooth but structured. In this setting, the graph of the generalized gradient of h can often be decomposed into a union (nondisjoint) of simpler subsets. "Identification" amounts to deciding which subsets of the graph are "active" in the criticality conditions at a given solution. We give conditions under which any convergent sequence of approximate critical points finitely identifies the activity. Prominent among these properties is a condition akin to the Mangasarian-Fromovitz constraint qualification, which ensures boundedness of the set of...

  20. Mathematical modeling of positron emission tomography (PET) data to assess radiofluoride transport in living plants following petiolar administration

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Converse, Alexander K.; Ahlers, Elizabeth O.; Bryan, Tom W.; Hetue, Jackson D.; Lake, Katherine A.; Ellison, Paul A.; Engle, Jonathan W.; Barnhart, Todd E.; Nickles, Robert J.; Williams, Paul H.; et al

    2015-01-01T23:59:59.000Z

    Background: Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transport in plants after leaf absorption is of interest to those in agricultural areas near industrial sources of airborne fluoride. Here we report the novel use of a commercial, high-resolution, animal positron emission tomography (PET) scanner to trace a bolus of [¹?F]fluoride administered via bisected petioles of Brassica oleracea, an established model species, to simulate whole plant uptake of atmospheric fluoride. This methodology allows for the first time mathematical compartmental modelingmore »of fluoride transport in the living plant. Radiotracer kinetics in the stem were described with a single-parameter free- and trapped-compartment model and mean arrival times at different stem positions were calculated from the free-compartment time-activity curves. Results: After initiation of administration at the bisected leaf stalk, [¹?F] radioactivity climbed for approximately 10 minutes followed by rapid washout from the stem and equilibration within leaves. Kinetic modeling of transport in the stem yielded a trapping rate of 1.5 +/- 0.3%/min (mean +/- s.d., n = 3), velocity of 2.2 +/- 1.1 cm/min, and trapping fraction of 0.8 +/- 0.5%/cm. Conclusion: Quantitative assessment of physiologically meaningful transport parameters of fluoride in living plants is possible using standard positron emission tomography in combination with petiolar radiotracer administration. Movement of free fluoride was observed to be consistent with bulk flow in xylem, namely a rapid and linear change in position with respect to time. Trapping, likely in the apoplast, was observed. Future applications of the methods described here include studies of transport of other ions and molecules of interest in plant physiology.« less

  1. Topology and Dynamics of Active Nematic Vesicles

    E-Print Network [OSTI]

    Felix C. Keber; Etienne Loiseau; Tim Sanchez; Stephen J. DeCamp; Luca Giomi; Mark J. Bowick; M. Cristina Marchetti; Zvonimir Dogic; Andreas R. Bausch

    2014-09-05T23:59:59.000Z

    Engineering synthetic materials that mimic the remarkable complexity of living organisms is a fundamental challenge in science and technology. We study the spatiotemporal patterns that emerge when an active nematicfilm of microtubules and molecular motors is encapsulated within a shape-changing lipid vesicle. Unlike in equilibrium systems, where defects are largely static structures, in active nematics defects move spontaneously and can be described as self-propelled particles. The combination of activity, topological constraints and vesicle deformability produces a myriad of dynamical states. We highlight two dynamical modes: a tunable periodic state that oscillates between two defect configurations, and shape-changing vesicles with streaming filopodia-like protrusions. These results demonstrate how biomimetic materials can be obtained when topological constraints are used to control the non-equilibrium dynamics of active matter.

  2. Evolution of an Early Illness Warning System to Monitor Frail Elders in Independent Living

    E-Print Network [OSTI]

    He, Zhihai "Henry"

    Evolution of an Early Illness Warning System to Monitor Frail Elders in Independent Living Gregory the evolution of an early illness warning system used by an interdisciplinary team composed of clinicians and engineers in an independent living facility. The early illness warning system consists of algorithms which

  3. Adapting the Home for Independent Living for the Elderly and Handicapped.

    E-Print Network [OSTI]

    Young, Sue

    1979-01-01T23:59:59.000Z

    * A feeling of independence is important to everyone, including the elderly and handicapped. The majority of elderly persons in Texas own their homes and wish to live independently as long as health permits. The self worth of the handicapped person... ..... 9 Bedroom ...................................... 10 Adaptation - Neither Expensive nor Difficult .... 10 References .................................... 10 ., ADAPTING THE HOME for independent living for the elderly and handicapped Sue Young...

  4. How did LUCA make a living? Chemiosmosis in the origin of life

    E-Print Network [OSTI]

    Allen, John F.

    necessary for carbon and energy metabolism in all free-living chemotrophs, and presum- ably the first free-living cells too. Proton gradients form naturally at alkaline hydrothermal vents and are viewed as central from the vents. Synthesis of ATP by chemi- osmosis today involves generation of an ion gradient

  5. Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer

    E-Print Network [OSTI]

    Fischer, Gerhard

    Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer University of Colorado as tools, particularly computational artifacts. We introduce the notions of tools for living and tools for the design of artifacts. The tools concept is then studied in the context of the Memory Aiding Prompting

  6. www.hfhl.umn.edu Healthy Foods, Healthy Lives (HFHL) Institute

    E-Print Network [OSTI]

    Amin, S. Massoud

    Lives (HFHL) Institute is an all-university Institute supported by funds from the University funding cycle (fall 2014) OR proposals may be prepared and submitted to other equivalent Universitywww.hfhl.umn.edu Healthy Foods, Healthy Lives (HFHL) Institute Planning Grant Program for Community-University

  7. Nature Macmillan Publishers Ltd 1997 insulating; and so on. A living polymer-

    E-Print Network [OSTI]

    Nature © Macmillan Publishers Ltd 1997 insulating; and so on. A living polymer- ization method . David A. Tirrell is in the Department of Polymer Science and Engineering, University of Massachusetts. Biopolymers 16, 2005­2028 (1977). 7. Szwarc, M. Carbanions, Living Polymers, and Electron-Transfer Processes

  8. The efficiency of mitochondrial electron transport chain is increased in the long-lived mrg19

    E-Print Network [OSTI]

    Babu, M. Madan

    The efficiency of mitochondrial electron transport chain is increased in the long-lived mrg19 mtROS and contribute to longevity. This increased mitochondrial efficiency (i.e. low mtROS generated the observed higher mito- chondrial efficiency in the long-lived mrg19 mutant of Saccharomyces cerevisiae

  9. Postdoctoral Positions In-vivo Optical Imaging and Microscopy of the Living Brain

    E-Print Network [OSTI]

    Adams, Mark

    Postdoctoral Positions In-vivo Optical Imaging and Microscopy of the Living Brain Columbia insight into the function and physiology of the living brain. We are particularly interested in exploring brain. Neurovascular coupling is important both because it is the basis of the fMRI BOLD signal

  10. Managing the Effect of Delay Jitter on the Display of Live Continuous Media

    E-Print Network [OSTI]

    Whitton, Mary C.

    Managing the Effect of Delay Jitter on the Display of Live Continuous Media Donald L. Stone of Delay Jitter on the Display of Live Continuous Media (under the direction of Kevin Jeffay). ABSTRACT latency in the presence of delay jitter, where delay jitter is defined as variation in processing

  11. Existence of long-lived isomeric states in naturally-occurring neutron-deficient Th isotopes

    E-Print Network [OSTI]

    A. Marinov; I. Rodushkin; Y. Kashiv; L. Halicz; I. Segal; A. Pape; R. V. Gentry; H. W. Miller; D. Kolb; R. Brandt

    2006-05-10T23:59:59.000Z

    Evidence for the existence of long-lived neutron-deficient isotopes has been found in a study of naturally-occurring Th using iductively coupled plasma-sector field mass spectrometry. They are interpreted as belonging to the recently discovered class of long-lived high spin super- and hyperdeformed isomers.

  12. Activity report

    SciTech Connect (OSTI)

    Yu, S W

    2008-08-11T23:59:59.000Z

    This report is aimed to show the author's activities to support the LDRD. The title is 'Investigation of the Double-C Behavior in the Pu-Ga Time-Temperature-Transformation Diagram' The sections are: (1) Sample Holder Test; (2) Calculation of x-ray diffraction patterns; (3) Literature search and preparing publications; (4) Tasks Required for APS Experiments; and (5) Communications.

  13. the Difference 1 the DifferenceYOUR GIVING CHANGES LIVES

    E-Print Network [OSTI]

    Birmingham, University of

    societies, allowing more students to participate in theatre and performance activities. Hydrogen train Studentship Burkitt's Lymphoma Students and young researchers at Birmingham built the world's first hydrogen locomotive engine. The train used cutting-edge propulsion technology and was entered in the first Institution

  14. From living cells to atoms Compartmentalisation in the cell

    E-Print Network [OSTI]

    Economou, Tassos

    ;Mitochondrial Energy MetabolismMitochondrial Energy Metabolism #12;The Electrochemical Proton Gradient metabolism enzymes,lipid metabolism enzymes, porinporin IMSIMS 6% of total6% of total mitThe Electrochemical Proton Gradient 140 mV 60 mV (-1 pH unit) TOTAL 200 mV #12;Active transport processes are driven

  15. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

    2011-02-15T23:59:59.000Z

    Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the “fines” fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

  16. Activation of building air in a Tokamak Engineering Test Facility

    SciTech Connect (OSTI)

    Leonard, B.R. Jr.; Perry, R.T.

    1980-09-01T23:59:59.000Z

    The production of radionuclides by neutron reactions in the building air of a conceptual Tokamak Engineering Test Facility has been calculated. The short-lived radionuclides /sup 13/N, /sup 16/N and /sup 41/Ar are all found to greatly exceed their maximum permissable concentration values. Longer-lived radionuclides /sup 3/H, /sup 14/C and /sup 39/Ar are also found to be produced in significant concentrations. The present results are compared with values calculated for three other fusion devices; TFTR, INS, and FMIT. These comparisons show that the ETF can be a prolific producer of activated air.

  17. Fluctuations and Rheology in Active Bacterial Suspensions D. T. N. Chen,1

    E-Print Network [OSTI]

    McGaughey, Alan

    the power spectrum of the active stress fluctuations. In some cases, we observe 1= ! p scaling in the noise Active complex fluid systems such as living cells [1,2], assemblies of motors and filaments [3], flocks of birds [4], and vibrated granular media [5] differ from conventional equilibrium media in that some

  18. Flexible Sensor Array Wraps Beating Hearts to Map Cardiac Activity in Real Time

    E-Print Network [OSTI]

    Rogers, John A.

    Flexible Sensor Array Wraps Beating Hearts to Map Cardiac Activity in Real Time By Jeremy Hsu Flexible Silicon Sensors Put this sensor on your heart and tell me it's all over University of Illinois Getting a cardiac map of the electrical activity coursing through a live, beating heart has proven

  19. MassLive.com's Printer-Friendly Page http://www.masslive.com/printer/printer.ssf?/base/living-0/11284120... 1 of 1 10/7/2005 12:47 PM

    E-Print Network [OSTI]

    Lovley, Derek

    nanowires, which are electrically conductive. The hope is Geobacter could be engineered to harvest a $21.8 million grant to continue his investigation into microbes that can harvest electricity from the microorganism Geobacter, specifically which of Geobacter's genes do what and in which environment

  20. Report on Second Activations with the Lead Slowing Down Spectrometer

    SciTech Connect (OSTI)

    Stave, Sean C.; Mace, Emily K.; Pratt, Sharon L.; Warren, Glen A.

    2012-04-27T23:59:59.000Z

    Summary On August 18 and 19 2011, five items were irradiated with neutrons using the Lead Slowing Down Spectrometer (LSDS). After irradiation, dose measurements and gamma-spectrometry measurements were completed on all of the samples. No contamination was found on the samples, and all but one provided no dose. Gamma-spectroscopy measurements qualitatively agreed with expectations based on the materials. As during the first activation run, we observed activation in the room in general, mostly due to 56Mn and 24Na. Most of the activation of the samples was short lived, with half-lives on the scale of hours to days, except for 60Co which has a half-life of 5.3 y.

  1. Search for long lived heaviest nuclei beyond the valley of stability

    E-Print Network [OSTI]

    P. Roy Chowdhury; C. Samanta; D. N. Basu

    2008-02-26T23:59:59.000Z

    The existence of long lived superheavy nuclei (SHN) is controlled mainly by spontaneous fission and $\\alpha$-decay processes. According to microscopic nuclear theory, spherical shell effects at Z=114, 120, 126 and N=184 provide the extra stability to such SHN to have long enough lifetime to be observed. To investigate whether the so-called "stability island" could really exist around the above Z, N values, the $\\alpha$-decay half lives along with the spontaneous fission and $\\beta$-decay half lives of such nuclei are studied. The $\\alpha$-decay half lives of SHN with Z=102-120 are calculated in a quantum tunneling model with DDM3Y effective nuclear interaction using $Q_\\alpha$ values from three different mass formulae prescribed by Koura, Uno, Tachibana, Yamada (KUTY), Myers, Swiatecki (MS) and Muntian, Hofmann, Patyk, Sobiczewski (MMM). Calculation of spontaneous fission (SF) half lives for the same SHN are carried out using a phenomenological formula and compared with SF half lives predicted by Smolanczuk {\\it et al}. Possible source of discrepancy between the calculated $\\alpha$-decay half lives of some nuclei and the experimental data of GSI, JINR-FLNR, RIKEN are discussed. In the region of Z=106-108 with N$\\sim$ 160-164, the $\\beta$-stable SHN $^{268}_{106}Sg_{162}$ is predicted to have highest $\\alpha$-decay half life ($T_\\alpha \\sim 3.2hrs$) using $Q_\\alpha$ value from MMM. Interestingly, it is much greater than the recently measured $T_\\alpha$ ($\\sim 22s$) of deformed doubly magic $^{270}_{108}Hs_{162}$ nucleus. A few fission-survived long-lived SHN which are either $\\beta$-stable or having large $\\beta$-decay half lives are predicted to exist near $^{294}110_{184}$, $^{293}110_{183}$, $^{296}112_{184}$ and $^{298}114_{184}$. These nuclei might decay predominantly through $\\alpha$-particle emission.

  2. 9/4/12 Tigers change behavior to live near people | TG Daily 1/9www.tgdaily.com/sustainability-features/65902-tigers-change-behavior-to-live-near-people

    E-Print Network [OSTI]

    9/4/12 Tigers change behavior to live near people | TG Daily 1/9www.tgdaily.com/sustainability change behavior to live near people | TG Daily 2/9www.tgdaily.com/sustainability-features/65902-tigers-change-behavior-to-live people tigers Advertisement #12;9/4/12 Tigers change behavior to live near people | TG Daily 3/9www.tgdaily.com/sustainability

  3. An estimated one of every six households (16.2 percent) in Texas lives in poverty. Research has

    E-Print Network [OSTI]

    Relevance An estimated one of every six households (16.2 percent) in Texas lives in poverty. Research has shown that individuals who live in poverty have dietary intakes that are not in agreement

  4. Forging an Ascetic Planet: Jesuit Lives and Virtues on the Mission Frontier of Eighteenth-Century New Spain

    E-Print Network [OSTI]

    Green, Bryan David

    2012-01-01T23:59:59.000Z

    Angeles Forging an Ascetic Planet: Jesuit Lives and VirtuesForging an Ascetic Planet: Jesuit Lives and Virtues on thebe a thoroughly “ascetic planet,” by which he meant that a

  5. Design for the Frail Old: Environmental and Perceptual Influences on Corridor Walking Behaviors of Assisted Living Residents

    E-Print Network [OSTI]

    Lu, Zhipeng

    2010-10-12T23:59:59.000Z

    living among these residents and yet, there is little research that has been done in this regard. The researcher conducted two studies in Central Texas to explore how corridor design features influenced indoor walking behaviors among assisted living...

  6. active root-inhabiting microbes: Topics by E-print Network

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    after solvents. Indigenous microorganisms were stimulated to degrade trichloroethylene (TCE), tetrachloroethylene to stimulate indigenous bacteria (methanotrophs) to degrade...

  7. LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Care Daily Checklist.docx revised 8/9/13 Page 1 of 1

    E-Print Network [OSTI]

    Langerhans, Brian

    LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Care Daily Checklist.docx revised 8/9/13 Page 1 of 1 Live Fish Care Daily Checklist D. Clark Labs rooms G-06 & G-08 morning visit: Turn) eggs from fridge in room 382; feed the live fish there Feed hatched Artemia (=brine shrimp) to fry

  8. Working lives of prison managers: exploring agency and structure in the late modern prison 

    E-Print Network [OSTI]

    Bennett, Jamie Stewart

    2012-11-27T23:59:59.000Z

    This study explores the contemporary working lives of prison managers. It attempts to understand the ways in which globalised changes in management practices have intersected with localised practices and occupational ...

  9. Use of encapsulated live microalgae to investigate pre-ingestive selection in the oyster Crassostrea gigas

    E-Print Network [OSTI]

    Allam, Bassem

    Use of encapsulated live microalgae to investigate pre-ingestive selection in the oyster, this study clearly demonstrate that extracellular metabolites produced by microalgae play a crucial role reserved. Keywords: Alginate; Beads; Bivalve; Feeding; Microalgae; Particle processing 1. Introduction

  10. New reporters of protein trafficking and protein-protein interactions in live cells

    E-Print Network [OSTI]

    Fernández Suárez, Marta

    2008-01-01T23:59:59.000Z

    Here, we describe our attempts to harness the exquisite specificity of natural protein and RNA enzymes to develop improved methods to study protein localization and protein-protein interactions in live cells. We first ...

  11. U.S. Bamboo house of the future : standardizing ecological living

    E-Print Network [OSTI]

    Wong, Lucy Lai

    2006-01-01T23:59:59.000Z

    This thesis focuses on ecological living through the use of bamboo. It explores how the material can be used for methods of prefabricated housing design within the United States. It also uses a "ht of parts" and describes ...

  12. Live Webinar on the Funding Opportunity for Technology Incubator for Wind Energy Innovations

    Broader source: Energy.gov [DOE]

    On April 17, 2014, from 1:00 – 3:00 PM MDT, the Wind Program will hold a live webinar to provide information to potential applicants for the Technology Incubator for Wind Energy Innovations Funding Opportunity.

  13. Camera that takes pictures of aircraft and ground vehicle tires can save lives

    E-Print Network [OSTI]

    Wiseman, Yair

    ;Camera that takes pictures of aircraft and ground vehicle tires can save lives Yair Wiseman Holon Institute of Technology Computer Science Department Holon 58102, Israel E-mail: wiseman

  14. Sustainability processes in community-level health initiatives: the experiences of Scottish healthy living centres 

    E-Print Network [OSTI]

    Rankin, David

    2010-01-01T23:59:59.000Z

    Background This thesis explores processes involved in stakeholders’ attempts to secure sustainability of three short-term funded community health initiatives known as healthy living centres (HLCs). The overall aim was ...

  15. M49-- Machine for the Living : a performance broadcast through an interfering FM radio transmission

    E-Print Network [OSTI]

    Goldfarb, Maximilian M

    2006-01-01T23:59:59.000Z

    The focus of the M49: Machine For The Living project is a performance work that considers the omnipresence of layered communications, which extend, yet supersede corporeal space of the individual. M49 creates a framework ...

  16. Live Webinar on the Funding Opportunity for Marine and Hydrokinetic Research and Development University Consortium

    Broader source: Energy.gov [DOE]

    On April 24, 2014 from 1:00 - 2:30 PM EDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Marine and Hydrokinetic (MHK) Research and...

  17. Using Schwartz Values to Assess Stakeholder Relations in the Coigach-Assynt Living Landscape 

    E-Print Network [OSTI]

    Burgener, Ariana Jude

    2014-11-27T23:59:59.000Z

    This dissertation explores stakeholder relations in an environmental management case study from Northwest Scotland that has aimed to employ the Ecosystem Approach in their methods. The Coigach-Assynt Living Landscape ...

  18. Making lives under closure : birth and medicine in Palestine's waiting zones

    E-Print Network [OSTI]

    Wick, Livia

    2006-01-01T23:59:59.000Z

    Reproduction is a site for understanding the ways in which people reconceptualize and re-organize the world in which they live. This dissertation tries to understand the world of birth under the regime of closures and ...

  19. Better Living with xlinkit Christian Nentwich, Wolfgang Emmerich and Anthony Finkelstein

    E-Print Network [OSTI]

    Finkelstein, Anthony

    Better Living with xlinkit Christian Nentwich, Wolfgang Emmerich and Anthony Finkelstein Department of Computer Science University College London, London WC1E 6BT {c.nentwich,w.emmerich

  20. ESA living planet symposium 28 June 2 July 2010, Bergen, Norway

    E-Print Network [OSTI]

    Stuttgart, Universität

    of unobserved GGs Stochastic model ESA living planet symposium 2010 Stochastic model High performance computing estimation stochastic model of invariants high performance computing error propagation synthesis performance computing environment Oliver Baur, Nico Sneeuw, Jianqing Cai, Matthias Roth Institute of Geodesy

  1. Reconstituting Lives: Somali Women's Efforts to Reformulate Household and Community Values in Kansas City, Missouri

    E-Print Network [OSTI]

    Filippi-Franz, Melissa

    2009-01-01T23:59:59.000Z

    The research addresses what Somali women living in Kansas City, Missouri do to assist their households and communities to come to terms with the consequences of forced migration. Women's contributions to wellbeing are found ...

  2. Sipping fuel and saving lives: increasing fuel economy without sacrificing safety

    E-Print Network [OSTI]

    Gordon, Deborah; Greene, David L.; Ross, Marc H.; Wenzel, Tom P.

    2008-01-01T23:59:59.000Z

    Ford delays plans to boost fuel economy of its SUVs. WallImproving safety without impacting fuel economy. Honda MotorCompany, October 4. SIPPING FUEL AND SAVING LIVES / 24

  3. COP-MAN --Perception for Mobile Pick-and-Place in Human Living Environments

    E-Print Network [OSTI]

    Cremers, Daniel

    COP-MAN -- Perception for Mobile Pick-and-Place in Human Living Environments Michael Beetz, Nico¨unchen, 85748, Germany {beetz, blodow, klank, marton, pangercic, rusu}@cs.tum.edu Abstract-- While many specific

  4. Comparison of photosynthetic responses of Ashe juniper and live oak on the Edwards Plateau, Texas 

    E-Print Network [OSTI]

    Bendevis, Mira Arpe

    2009-06-02T23:59:59.000Z

    gas exchange and water relations at the leaf level of the two dominant tree species, Ashe juniper and live oak (Quercus virginiana P. Mill. Var. fusiformis), is important to assess the impact of juniper encroachment on the aquifer. Photosynthesis...

  5. Live Webcast on the 2014 Farm Bill's Renewable Energy for America Program

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “The 2014 Farm Bill's Renewable Energy for America Program” on May 21, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  6. Live Webinar on the Funding Opportunity for Wind Forecasting Improvement Project in Complex Terrain

    Broader source: Energy.gov [DOE]

    On April 21, 2014 from 3:00 to 5:00 PM EST the Wind Program will hold a live webinar to provide information to potential applicants for this Funding Opportunity Announcement. There is no cost to...

  7. A descriptive analysis of the Live Animal and Carcass Evaluation Service (LACES)

    E-Print Network [OSTI]

    Oman, Jennifer Stamline

    1991-01-01T23:59:59.000Z

    B LACES COMPUTER ANALYSIS 39 LIVE ANIMAL AND CARCASS EVALUATION SERVICE Texas ASM University System Department of Animal Science College Station, Texas 77840 (409) 845-3935 Date: 03/26/91 To: SAMPLE RUN This report by the Beef Cattle Science... is sincerely appreciated and you are invited to call if there are any questions. Thank you. Data recorded and summarized by Dr. Bill Turner LACES Report and billing verifaed by Lorna Pelton 40 LIVE ANIMAL AND CARCASS EVALUATION SERVICE DATE: 03...

  8. Prediction of intramuscular fat in live and slaughtered beef animals through processing of ultrasonic images

    E-Print Network [OSTI]

    Thane, Brian Ray

    1992-01-01T23:59:59.000Z

    PREDICTION OF INTRAMUSCULAR FAT IN LIVE AND SLAUGHTERED BEEF ANIMALS THROUGH PROCESSING OF ULTRASONIC IMAGES A Thesis by BRIAN RAY THANE Submitted to the Office of Graduate Studies of Texas ARM University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE May 1992 Major Subject: Agricultural Engineering PREDICTION OF INTRAMUSCULAR FAT IN LIVE AND SLAUGHTERED BEEF ANIMALS THROUGH PROCESSING OF ULTRASONIC IMAGES A Thesis by BRIAN RAY THANK Approved...

  9. The use of ultrasonic techniques in estimating carcass traits in live beef animals

    E-Print Network [OSTI]

    Wideman, Donald

    1963-01-01T23:59:59.000Z

    THE USE OF ULTRASONIC TECHNIQUES IN ESTIMATING CARCASS TRAITS IN LIVE BEEF ANIMALS A Thesis By DONALD WIDEMAN Submitted to the Graduate School of the Agricultural and Mechanical College of Te~s in partial fulfillment of the requirements... for the degree of MASTER OF SCIENCE May 1963 Major Subject: Animal Breeding THE USE OF ULTRASONIC TECHNIQUES IN ESTIMATING CARCASS TRAITS IN LIVE BEEF ANIMALS A Thesis By DONALD WIDEMAN Approved as to style and content by: (Chairman of Committ ) (Head...

  10. Vegetation response to burning thicketized live oak savannah on the Aransas National Wildlife Refuge

    E-Print Network [OSTI]

    Kelley, David Mitchell

    1980-01-01T23:59:59.000Z

    VEGETATION RESPONSE TO BURNING THICKETIZED LIVE OAK SAVANNAH ON THE ARANSAS NATIONAL WILDLIFE REFUGE A Thesi. s by DAVID MITCHELL KELLEY Submitted to the Graduate College of Texas A 6 M University in partial fulfillment of the requirement... irma f Committee) (Head of Department) (Member) ( lambe-) (Member) Memos Memoerl '. !ay 98O ABSTRACT Vegetation Response to Burning Thicketized Live Oak Savannah on the Aransas National Wildlife Refuge (May 1980) David Mitchell Kelley, B. S...

  11. The Role of Acculturation in Nutrition Behaviors among Low Income Hispanic Women Living in Texas

    E-Print Network [OSTI]

    Atehortua, Nelson

    2012-10-19T23:59:59.000Z

    THE ROLE OF ACCULTURATION IN NUTRITION BEHAVIORS AMONG LOW INCOME HISPANIC WOMEN LIVING IN TEXAS A Dissertation by NELSON ALBERTO ATEHORTUA Submitted to the Office of Graduate Studies of Texas A&M University in partial... in Texas Copyright 2012 Nelson Alberto Atehortua THE ROLE OF ACCULTURATION IN NUTRITION BEHAVIORS AMONG LOW INCOME HISPANIC WOMEN LIVING IN TEXAS A Dissertation by NELSON ALBERTO ATEHORTUA Submitted to the Office of Graduate Studies...

  12. Influence of top removal on tebuthiuron efficacy for live oak control

    E-Print Network [OSTI]

    Swetish, Stephen Michael

    1986-01-01T23:59:59.000Z

    OF CONTENTS. LIST OF TABLES. INTRODUCTION. LITERATURE REVIEW. MATERIALS AND METHODS. Site Description. Experimental Design. Rainfall Data. Live Oak Response. Forage Standing Crops. Herbaceous Cover. RESULTS AND DISCUSSION. Rainfall Data. Live Oak... AND METHODS Site Description This study was conducted on the Sibley Estate near Edna in Lavaca County, Texas. It is located in the transition zone between the Gulf Coast Prairies and Marshes and the Post Oak Savanna Vegetational Areas of Texas (Gould 1975...

  13. Texture and Color Distribution-basedTexture and Color Distribution-based Classification for Live Coral DetectionClassification for Live Coral Detection

    E-Print Network [OSTI]

    Stough, Joshua

    Coral DetectionClassification for Live Coral Detection Joshua V. Stough, Lisa Greer, Matt BensonJoshua V, ... · Coral benthic studiesCoral benthic studies ­ NCC/LBP with NN ­NCC/LBP with NN ­ [Marcos et al., Optics

  14. nAture methods | VOL.10 NO.2 | FEBRUARY2013 | 147 measurement of protease activity in living cells or organisms

    E-Print Network [OSTI]

    Cai, Long

    , such as gout6, atherosclerosis7, type 2 diabetes8 and Alzheimer's disease9, engagement of the NLRP3­ASC

  15. Fishing activity, health characteristics and mercury exposure of Amerindian women living alongside the Beni River (Amazonian Bolivia)

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    the Beni River (Amazonian Bolivia) Authors: Eric Benefice 1 ; Selma Luna - Monrroy 1, 2 Ronald Lopez de Ciencias Bioquímicas y Farmacéuticas, Universidad Mayor de San Andrés, La Paz, Bolivia) 3 INLASA, (Instituto Nacional de Laboratorios en Salud, Ministerio de Salud y Deportes), La Paz (Bolivia) Corresponding

  16. Molecular Imaging of the ATM Kinase Activity

    SciTech Connect (OSTI)

    Williams, Terence M. [Department of Radiation Oncology, Ohio State University, Columbus, Ohio (United States); Nyati, Shyam [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Ross, Brian D. [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Rehemtulla, Alnawaz, E-mail: alnawaz@umich.edu [Department of Radiation Oncology, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Center for Molecular Imaging, University of Michigan Medical Center, Ann Arbor, Michigan (United States); Department of Radiology, University of Michigan Medical Center, Ann Arbor, Michigan (United States)

    2013-08-01T23:59:59.000Z

    Purpose: Ataxia telangiectasia mutated (ATM) is a serine/threonine kinase critical to the cellular DNA-damage response, including from DNA double-strand breaks. ATM activation results in the initiation of a complex cascade of events including DNA damage repair, cell cycle checkpoint control, and survival. We sought to create a bioluminescent reporter that dynamically and noninvasively measures ATM kinase activity in living cells and subjects. Methods and Materials: Using the split luciferase technology, we constructed a hybrid cDNA, ATM-reporter (ATMR), coding for a protein that quantitatively reports on changes in ATM kinase activity through changes in bioluminescence. Results: Treatment of ATMR-expressing cells with ATM inhibitors resulted in a dose-dependent increase in bioluminescence activity. In contrast, induction of ATM kinase activity upon irradiation resulted in a decrease in reporter activity that correlated with ATM and Chk2 activation by immunoblotting in a time-dependent fashion. Nuclear targeting improved ATMR sensitivity to both ATM inhibitors and radiation, whereas a mutant ATMR (lacking the target phosphorylation site) displayed a muted response. Treatment with ATM inhibitors and small interfering (si)RNA-targeted knockdown of ATM confirm the specificity of the reporter. Using reporter expressing xenografted tumors demonstrated the ability of ATMR to report in ATM activity in mouse models that correlated in a time-dependent fashion with changes in Chk2 activity. Conclusions: We describe the development and validation of a novel, specific, noninvasive bioluminescent reporter that enables monitoring of ATM activity in real time, in vitro and in vivo. Potential applications of this reporter include the identification and development of novel ATM inhibitors or ATM-interacting partners through high-throughput screens and in vivo pharmacokinetic/pharmacodynamic studies of ATM inhibitors in preclinical models.

  17. Retrospective radon progeny measurements through measurements of 210 activities on glass objects using stacked LR 115 detectors

    E-Print Network [OSTI]

    Yu, K.N.

    Retrospective radon progeny measurements through measurements of 210 Po activities on glass objects 2 September 2008 PACS: 29.40 23.60 Keywords: Radon Radon progeny 210 Po Implantation Retrospective for determining 210 Po activity in glass surfaces after deposition of short-lived radon progeny. The sensitivities

  18. Assessing the Quality of Activities in a Smart Environment D.J. Cook and M. Schmitter-Edgecombe

    E-Print Network [OSTI]

    Cook, Diane J.

    are used to recognize activities that are performed in a smart home and to identify errors using machine learning algorithms and smart home technology. These algorithms will be useful for automating remote health monitoring and interventions. Keywords: activities of daily living, smart homes

  19. This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research

    E-Print Network [OSTI]

    Coley, Phyllis

    and endophyte were collected. The extract was inactive to the human parasites Trypanosoma cruzi, Leishmania reserved. Introduction Endophytes are microorganisms including bacteria or fungi that live within,2 Endophytic microbes have been recognized as important sources of structurally novel and biologically active

  20. Respiratory survey of North American Indian children living in proximity to an aluminum smelter

    SciTech Connect (OSTI)

    Ernst, P.; Thomas, D.; Becklake, M.R.

    1986-02-01T23:59:59.000Z

    We explored the relationship of respiratory symptoms and lung function to exposure to ambient air pollution consisting of particulate and gaseous fluorides. The subjects were 253 North American Indian children 11 to 17 yr of age living on the Akwasasne reserve, which is adjacent to an aluminum smelter. Among boys, closing volume (CV/VC%) was increased in those raised closest to the smelter as opposed to those having lived most of their lives farthest from this source of air pollution. In both sexes, there was a significant linear relationship between increasing CV/VC% and the amount of fluoride contained in a spot urine sample. We conclude that exposure to fluoride air pollution in the community may be associated with abnormalities in small airways. The implication of these abnormalities for future respiratory health is unknown.

  1. Application of neutron activation analysis and high resolution x-ray spectrometry for the determination of trace quantities of elements with short-lived activation products

    E-Print Network [OSTI]

    Marshall, John Richard

    1974-01-01T23:59:59.000Z

    its energy by ejecting an electron from the K, L, or M shell. As in the case of elect. ron capture, this vacated shell is filled by electrons from hi, ghez energy levels resulting in the emission of' characteristic x-rays. It was H. G. J. Mosely... interacts with orbital electrons. This interaction causes a transfer of energy and angular momentum to an orbital electron of the K, L or M shell, thus causing it to be ejected from the atom. The ejected "conversion electron" will be emitted...

  2. Application of neural networking in live cattle futures market: an approach to price-forecasting

    E-Print Network [OSTI]

    Chou, Chien-Ju

    1993-01-01T23:59:59.000Z

    APPLICATION OF NEURAL NETWORKING IN LIVE CATTLE FUTURES MARKET: AN APPROACH TO PRICE-FORECASTING A Thesis by CHIEN-JU CHOU Submitted to the Office of Graduate Studies of Texas ARM University in partial fulfilhnent of the requirements... for the degree of MASTER OF SCIENCE August 1993 Major Subject: Animal Science APPLICATION OF NEURAL NETWORKING IN LIVE CATTLE FUTURES MARKET: AN APPROACH TO PRICE-FORECASTING A Thesis by CHIBN-JU CHOU Approved as to style and content by: John P. Walter...

  3. Calculation of cluster decays half-lives for nuclei between 56 temperature dependent proximity model

    E-Print Network [OSTI]

    Vahid Zanganeh

    2014-08-20T23:59:59.000Z

    Cluster decays half-lives of elements with proton numbers between 56 temperature dependent proximity potential approach. for showing the influence of temperature on cluster decays, we compared the results among temperature dependent and inde-pendent case with experimental values. The obtained results of the present investigation reveal that we have more accurate results for temperature dependent proximity potential in comparison to ignoring one. In the present work, we find that results provided with temperature dependent prox-imity model are reasonable estimates for cluster decays half-lives and provide reliable predictions for other super heavies cluster decays.

  4. Existence of long-lived isotopes of a superheavy element in natural Au

    E-Print Network [OSTI]

    A. Marinov; I. Rodushkin; A. Pape; Y. Kashiv; D. Kolb; R. Brandt; R. V. Gentry; H. W. Miller; L. Halicz; I. Segal

    2007-02-25T23:59:59.000Z

    Evidence for the existence of long-lived isotopes with atomic mass numbers 261 and 265 and abundance of (1-10)x10$^{-10}$ relative to Au has been found in a study of natural Au using an inductively coupled plasma - sector field mass spectrometer. The measured masses fit the predictions made for the masses of $^{261}$Rg and $^{265}$Rg (Z=111) and for some isotopes of nearby elements. The possibility that these isotopes belong to the recently discovered class of long-lived high spin super- and hyperdeformed isomeric states is discussed.

  5. On spontaneous fission and alpha-decay half-lives of atomic nuclei

    E-Print Network [OSTI]

    K. Pomorski; M. Warda; A. Zdeb

    2015-01-16T23:59:59.000Z

    It is shown, that the Gamow-like model with only one adjustable parameter - radius constant is able to reproduce well the alpha-decay half-lives for all even-even nuclei with the proton number larger than 50. The systematics for odd-A and odd-odd isotopes can be also well described when ones introduces an additional hindrance factor. Similar model based on the W. J. Swiatecki idea from 1955 is developed to reproduce the spontaneous fission half-lives of transactinide nuclei. The achieved accuracy of reproduction of the data is better than that obtained in more advanced theories.

  6. Phase separation and coarsening in active matter

    E-Print Network [OSTI]

    Giuseppe Gonnella; Davide Marenduzzo; Antonio Suma; Adriano Tiribocchi

    2015-02-08T23:59:59.000Z

    Active systems, or active matter, are self-driven systems which live, or function, far from equilibrium - a paradigmatic example which we focus on here is provided by a suspension of self-motile particles. Active systems are far from equilibrium because their microscopic constituents constantly consume energy from the environment in order to do work, for instance to propel themselves. The nonequilibrium nature of active matter leads to a variety of non-trivial intriguing phenomena. An important one which has recently been the subject of intense interest among biological and soft matter physicists is that of the so-called "motility-induced phase separation", whereby self-propelled particles accumulate into clusters in the absence of any explicit attractive interactions between them. Here we review the physics of motility-induced phase separation, and discuss this phenomenon within the framework of the classic physics of phase separation and coarsening. We also discuss cases where the coarsening may be arrested, either in theories for bacterial colonies or in experiments. Most of this work will focus on the case of run-and-tumble and active Brownian particles in the absence of solvent-mediated hydrodynamic interactions - we will briefly discuss at the end their role, which is not currently fully understood in this context.

  7. Association of airborne moisture-indicating microorganisms withbuilding-related symptoms and water damage in 100 U.S. office buildings:Analyses of the U.S. EPA BASE data

    SciTech Connect (OSTI)

    Mendell, Mark J.; Lei, Quanhong; Cozen, Myrna O.; Shendell, DerekG.; Macher, Janet M.; Tsai, Feng C.

    2003-10-01T23:59:59.000Z

    Metrics of culturable airborne microorganisms for either total organisms or suspected harmful subgroups have generally not been associated with symptoms among building occupants. However, the visible presence of moisture damage or mold in residences and other buildings has consistently been associated with respiratory symptoms and other health effects. This relationship is presumably caused by adverse but uncharacterized exposures to moisture-related microbiological growth. In order to assess this hypothesis, we studied relationships in U.S. office buildings between the prevalence of respiratory and irritant symptoms, the concentrations of airborne microorganisms that require moist surfaces on which to grow, and the presence of visible water damage. For these analyses we used data on buildings, indoor environments, and occupants collected from a representative sample of 100 U.S. office buildings in the U.S. Environmental Protection Agency's Building Assessment Survey and Evaluation (EPA BASE) study. We created 19 alternate metrics, using scales ranging from 3-10 units, that summarized the concentrations of airborne moisture-indicating microorganisms (AMIMOs) as indicators of moisture in buildings. Two were constructed to resemble a metric previously reported to be associated with lung function changes in building occupants; the others were based on another metric from the same group of Finnish researchers, concentration cutpoints from other studies, and professional judgment. We assessed three types of associations: between AMIMO metrics and symptoms in office workers, between evidence of water damage and symptoms, and between water damage and AMIMO metrics. We estimated (as odds ratios (ORs) with 95% confidence intervals) the unadjusted and adjusted associations between the 19 metrics and two types of weekly, work-related symptoms--lower respiratory and mucous membrane--using logistic regression models. Analyses used the original AMIMO metrics and were repeated with simplified dichotomized metrics. The multivariate models adjusted for other potential confounding variables associated with respondents, occupied spaces, buildings, or ventilation systems. Models excluded covariates for moisture-related risks hypothesized to increase AMIMO levels. We also estimated the association of water damage (using variables for specific locations in the study space or building, or summary variables) with the two symptom outcomes. Finally, using selected AMIMO metrics as outcomes, we constructed logistic regression models with observations at the building level to estimate unadjusted and adjusted associations of evident water damage with AMIMO metrics. All original AMIMO metrics showed little overall pattern of unadjusted or adjusted association with either symptom outcome. The 3-category metric resembling that previously used by others, which of all constructed metrics had the largest number of buildings in its top category, was not associated with symptoms in these buildings. However, most metrics with few buildings in their highest category showed increased risk for both symptoms in that category, especially metrics using cutpoints of >100 but <500 colony-forming units (CFU)/m{sup 3} for concentration of total culturable fungi. With AMIMO metrics dichotomized to compare the highest category with all lower categories combined, four metrics had unadjusted ORs between 1.4 and 1.6 for both symptom outcomes. The same four metrics had adjusted ORs of 1.7-2.1 for both symptom outcomes. In models of water damage and symptoms, several specific locations of past water damage had significant associations with outcomes, with ORs ranging from 1.4-1.6. In bivariate models of water damage and selected AMIMO metrics, a number of specific types of water damage and several summary variables for water damage were very strongly associated with AMIMO metrics (significant ORs ranging above 15). Multivariate modeling with the dichotomous AMIMO metrics was not possible due to limited numbers of observations.

  8. The isolation and evaluation of endophytic bacteria from live oaks as potential biological control agents for oak wilt in Texas

    E-Print Network [OSTI]

    Brooks, David Stewart

    1989-01-01T23:59:59.000Z

    on in vitro inhibition data, six isolates, three Gram-positive and three Gram-negative, were selected for colonization studies in container-grown red (()nereus texana) and live oak trees. Antibiotic resistant segregants, which allowed isolation on selective... in container-grown oak trees. Two experiments (Exp. i and II) were conducted with live oaks and one experiment with red oaks. In Exp. I, P. denitrificans significantly reduced the number of live oak trees expressing disease symptoms, while P. lucida showed...

  9. Tuned, driven, and active soft matter

    E-Print Network [OSTI]

    Andreas M. Menzel

    2015-01-28T23:59:59.000Z

    One characteristic feature of soft matter systems is their strong response to external stimuli. As a consequence they are comparatively easily driven out of their ground state and out of equilibrium, which leads to many of their fascinating properties. Here, we review illustrative examples. This review is structured by an increasing distance from the equilibrium ground state. On each level, examples of increasing degree of complexity are considered. In detail, we first consider systems that are quasi-statically tuned or switched to a new state by applying external fields. These are common liquid crystals, liquid crystalline elastomers, or ferrogels and magnetic elastomers. Next, we concentrate on systems steadily driven from outside e.g. by an imposed flow field. In our case, we review the reaction of nematic liquid crystals, of bulk-filling periodically modulated structures such as block copolymers, and of localized vesicular objects to an imposed shear flow. Finally, we focus on systems that are "active" and "self-driven". Here our range spans from idealized self-propelled point particles, via sterically interacting particles like granular hoppers, via microswimmers such as self-phoretically driven artificial Janus particles or biological microorganisms, via deformable self-propelled particles like droplets, up to the collective behavior of insects, fish, and birds. As we emphasize, similarities emerge in the features and behavior of systems that at first glance may not necessarily appear related. We thus hope that our overview will further stimulate the search for basic unifying principles underlying the physics of these soft materials out of their equilibrium ground state.

  10. Secondary Energy Infobook Activities (19 Activities)'

    Broader source: Energy.gov (indexed) [DOE]

    Infobook Activities (19 Activities) Grades: 9-12 Topics: Energy Basics Owner: NEED This educational material is brought to you by the U.S. Department of Energy's Office of Energy...

  11. Activated Boron Nitride Derived from Activated Carbon

    E-Print Network [OSTI]

    Zettl, Alex

    combination of chemical, thermal, and electrical properties. The utility of activated carbon suggests is characterized by scanning electron microscopy, high-resolution transmission electron microscopy, electron energy loss spectroscopy, and surface area analysis. The activated BN microstructure is similar

  12. Secret lives, public lies : the conversos and socio-religious non-conformism in the Spanish Golden Age

    E-Print Network [OSTI]

    Ingram, Kevin

    2006-01-01T23:59:59.000Z

    Sanford, Lost Lexicon: Secret Meanings in the Vocabulary ofOF CALIFORNIA, SAN DIEGO Secret Lives, Public Lies: TheChapter 6: Diego Velázquez’s Secret History…………………………………

  13. A Living Mediterranean River: Restoration and Management of the Rio Real in Portugal to Achieve Good Ecological Condition

    E-Print Network [OSTI]

    Natali, Jennifer; Kondolf, G. Mathias; Landeiro, Clara; Christian-Smith, Juliet; Grantham, Ted

    2009-01-01T23:59:59.000Z

    for stormwater treatment wetlands A LIVING MEDITERRANEANharvesting and water treatment wetlands using the theSITES for stormwater treatment wetland areas were identified

  14. Giant Volume Change of Active Gels under Continuous Flow Ye Zhang,,

    E-Print Network [OSTI]

    Epstein, Irving R.

    of chemomechanically active systems based on combining soft materials and microfluidic systems. INTRODUCTION Supporting Information ABSTRACT: While living systems have developed highly efficient ways to convert a challenge to build muscle-like biomimetic systems to generate mechanical force directly from chemical

  15. 20 Reasons Why Your Child Needs You to Be an Active Father

    E-Print Network [OSTI]

    in the development of their children. The latest research indicates that fathers who are actively involved in raising their children can make a positive and lasting difference in their lives. In contrast, this same research reveals demonstrate their love by spending quality and quantity time together. Children who feel loved are more likely

  16. An active queue management scheme to contain high bandwidth flows at a congested router

    E-Print Network [OSTI]

    Smitha

    2001-01-01T23:59:59.000Z

    be incorporated in RED, an active queue management scheme. The proposed scheme possesses all the advantages of RED. In addition, it lowers the drop rates of short-lived flows and also those high bandwidth flows that reduce their sending rate when congestion...

  17. Health Monitoring in an Agent-Based Smart Home by Activity Prediction

    E-Print Network [OSTI]

    Cook, Diane J.

    Health Monitoring in an Agent-Based Smart Home by Activity Prediction Sajal K. Das and Diane J objective of this paper is to investigate techniques for using agent-based smart home technologies-based smart home project funded by NSF. 1 Introduction and Motivation We live in an increasingly connected

  18. Living and working in Germany A guide for international scientists at Max Planck Institutes

    E-Print Network [OSTI]

    Living and working in Germany A guide for international scientists at Max Planck Institutes #12 and the hospitality in your town. You will quickly find that Germany is a country of many facets ­ a country Planck Society is Germany's most successful scientific or- ganisation in basic research. Max Planck

  19. LiveChat Wed, 11/16, 2 pm ET: Industrial Energy Efficiency

    Broader source: Energy.gov [DOE]

    On Wednesday, November 16, at 2:00 PM ET, join Kathleen Hogan, Deputy Assistant Secretary for Energy Efficiency, for a live discussion about the Energy Department programs how businesses are lowering their energy consumption, and how that energy savings benefits the American economy.

  20. Living Longer on Less THe neW economic (in)securiTy of seniors

    E-Print Network [OSTI]

    Snider, Barry B.

    Living Longer on Less THe neW economic (in)securiTy of seniors INSTITUTE ON ASSETS & SOCIAL POLICY to measuring economic security applied in this report builds on previous work on middle class economic security for Social Policy and Manage- ment at Brandeis University, is dedicated to the economic well-being and social

  1. @scale: Insights from a Large, Long-Lived Appliance Stephen Dawson-Haggerty

    E-Print Network [OSTI]

    Culler, David E.

    Design, Measurement, Performance Keywords Energy, Audit, Building, Power, Wireless, Sensor Network 1@scale: Insights from a Large, Long-Lived Appliance Energy WSN Stephen Dawson-Haggerty , Steven Lanzisera , Jay Taneja , Richard Brown , and David Culler Computer Science Division Environmental Energy

  2. Search for long-lived gravitational-wave transients coincident with long gamma-ray bursts

    E-Print Network [OSTI]

    Aggarwal, Nancy

    Long gamma-ray bursts (GRBs) have been linked to extreme core-collapse supernovae from massive stars. Gravitational waves (GW) offer a probe of the physics behind long GRBs. We investigate models of long-lived (?10–1000??s) ...

  3. LiveBench-2: Large-Scale Automated Evaluation of Protein Structure Prediction Servers

    E-Print Network [OSTI]

    Fischer, Daniel

    LiveBench-2: Large-Scale Automated Evaluation of Protein Structure Prediction Servers Janusz M from other evaluation experiments because it is a large-scale and a fully automated procedure. Since, to keep in pace with the development, we present the results of the second large-scale evaluation of pro

  4. We don't just teach sustainability--we live it!

    E-Print Network [OSTI]

    #12;We don't just teach sustainability-- we live it! Green comes to life every day at the University of Oregon's Center for Sustainable Business Practices. Housed at the Lundquist College of Business, and financial responsibilities. For us, the market demand for transparency, sustainability, and business

  5. Origin and Phylogeny of Microbes Living in Permanent Antarctic Lake Ice

    E-Print Network [OSTI]

    Priscu, John C.

    Origin and Phylogeny of Microbes Living in Permanent Antarctic Lake Ice D. A. Gordon,1, * J. Priscu of bacteria and cyanobacteria colonizing sediment particles in the per- manent ice cover of an Antarctic lake collected from a depth of 2.5 m in the 4-m-thick ice cover of Lake Bonney, McMurdo Dry Valleys, Antarctica

  6. "Cut Me Some Slack": Latency-Aware Live Migration for Databases

    E-Print Network [OSTI]

    Massachusetts at Amherst, University of

    by leveraging `migration slack', or resources that can be used for migration without excessively impacting query"Cut Me Some Slack": Latency-Aware Live Migration for Databases Sean Barker Yun Chi Hyun Jin Moon and exploit migration slack in real time. Using our prototype, we demonstrate that Slacker effectively

  7. Does Growth Rate Determine the Rate of Metabolism in Shorebird Chicks Living in the Arctic?

    E-Print Network [OSTI]

    Williams, Jos. B.

    primarily of greater metabolic inten- sities of heat-generating tissues. The maximum temperature gradient500 Does Growth Rate Determine the Rate of Metabolism in Shorebird Chicks Living in the Arctic/22/2007; Electronically Published 7/13/2007 ABSTRACT We measured resting and peak metabolic rates (RMR and PMR

  8. Measuring protein mobility by photobleaching GFP-chimeras in living cells

    E-Print Network [OSTI]

    Snapp, Erik Lee

    1 Measuring protein mobility by photobleaching GFP-chimeras in living cells Erik Snapp, Nihal Altan the fluorophore's fluorescence. The diffusive characteristics of green fluorescent protein (GFP)- chimeras, in which the same region of a cell expressing a GFP-chimera is repetitively photobleached and the loss

  9. Cold Climate Heat Pump Projects at Purdue University & the Living Lab

    E-Print Network [OSTI]

    Oak Ridge National Laboratory

    Cold Climate Heat Pump Projects at Purdue University & the Living Lab at the new Herrick Labs West Lafayette, Indiana 11/10/2011 1 IEA Heat Pump Program Executive Committee Meeting ASHRAE Headquarters, Atlanta, GA November 9, 2011 #12;Cold Climate Heat Pump April 1, 2010 ­ March 30, 2012 PIs

  10. Top-down and bottom-up diversity cascades in detrital vs. living food webs

    E-Print Network [OSTI]

    Dyer, Lee

    REPORT Top-down and bottom-up diversity cascades in detrital vs. living food webs Lee A. Dyer1 for maintaining diversity in biotic communities, but the indirect (ÔcascadingÕ) effects of top-down and bottom in decomposer food webs. We measured effects of top predators and plant resources on the diversity of endophytic

  11. The University of New Hampshire in 2020: Breaking Silos, Transforming Lives, Reimagining the University

    E-Print Network [OSTI]

    their historic mission and respond energetically and effectively to these new challenges? The University of New1 The University of New Hampshire in 2020: Breaking Silos, Transforming Lives, Reimagining of our state, our nation and our world. For 143 years we have served the people and communities of New

  12. LiveLab: Measuring Wireless Networks and Smartphone Users in the Field

    E-Print Network [OSTI]

    Zhong, Lin

    LiveLab: Measuring Wireless Networks and Smartphone Users in the Field Clayton Shepard*, Ahmad and wireless networks with a reprogrammable in- device logger designed for long-term user studies. We discuss of smartphone usage and measurement of wireless networks x In-field programmability of the logger so

  13. LiveLab: Measuring Wireless Networks and Smartphone Users in the Field

    E-Print Network [OSTI]

    Zhong, Lin

    1 LiveLab: Measuring Wireless Networks and Smartphone Users in the Field Clayton Shepard*, Ahmad and wireless networks with a reprogrammable in- device logger designed for long-term user studies. We discuss to measure smartphone users in the field and to measure wireless networks with smartphone users. The key

  14. Glacier-and permafrost-related hazards increasingly threaten human lives,settlements,

    E-Print Network [OSTI]

    Kääb, Andreas

    Glacier- and permafrost-related hazards increasingly threaten human lives where surface and sub-surface ice are involved.Changes in glacier and permafrost equilibrium in the cryospheric system.A number of recent glacier hazards and disasters underscore these trends.Difficult site

  15. Systematic calculations of $\\alpha$-decay half-lives with an improved empirical formula

    E-Print Network [OSTI]

    Wang, Z Y; Liu, Q; Guo, J Y

    2015-01-01T23:59:59.000Z

    Based on the recent data in NUBASE2012, an improved empirical formula for evaluating the $\\alpha$-decay half-lives is presented, in which the hindrance effect resulted from the change of the ground state spins and parities of parent and daughter nuclei is included, together with a new correction factor for nuclei near the shell closures. The calculated $\\alpha$-decay half-lives are found to be in better agreements with the experimental data, and the corresponding root-mean-square (rms) deviation is reduced to $0.433$ when the experimental $Q$-values are employed. Furthermore, the $Q$-values derived from different nuclear mass models are used to predict $\\alpha$-decay half-lives with this improved formula. It is found that the calculated half-lives are very sensitive to the $Q$-values. Remarkably, when mass predictions are improved with the radial basis function (RBF), the resulting rms deviations can be significantly reduced. With the mass prediction from the latest version of Weizs\\"{a}cker-Skyrme (WS4) mode...

  16. Heart stopping moments with zebrafish: imaging inside the living, beating heart

    E-Print Network [OSTI]

    Greenaway, Alan

    Heart stopping moments with zebrafish: imaging inside the living, beating heart Seamless this application, enabling researchers to carry out precision "healing" studies on the moving heart by targeting of development Jonathan M. Taylor, Glasgow University d "Realtime Optical Gating for 3D Heart Imaging", Journal

  17. The living workplace : a conscious work environment for a small publishing company

    E-Print Network [OSTI]

    Berg, Richard Carl

    1986-01-01T23:59:59.000Z

    This thesis is about the oneness of working and living, and about the making of workplaces that support and encourage the idea that one's work can be an integral part of one's life. The opening position is that there is a ...

  18. The Pollution Attack in P2P Live Video Streaming: Measurement Results and Defenses

    E-Print Network [OSTI]

    Saxena, Nitesh

    The Pollution Attack in P2P Live Video Streaming: Measurement Results and Defenses Prithula Dhungel in the recent years. In this paper, we examine the stream pollution attack, for which the attacker mixes polluted chunks into the P2P distribu- tion, degrading the quality of the rendered media at the receivers

  19. A C. elegans-based foam for rapid on-site detection of residual live virus.

    SciTech Connect (OSTI)

    Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

    2012-02-01T23:59:59.000Z

    In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

  20. DOES HOSPITAL COMPETITION SAVE LIVES? EVIDENCE FROM THE ENGLISH NHS PATIENT CHOICE REFORMS*

    E-Print Network [OSTI]

    Mateo, Jill M.

    DOES HOSPITAL COMPETITION SAVE LIVES? EVIDENCE FROM THE ENGLISH NHS PATIENT CHOICE REFORMS* Zack Cooper, Stephen Gibbons, Simon Jones and Alistair McGuire Recent substantive reforms to the English with fixed prices. This study investigates whether these reforms led to improvements in hospital quality. We

  1. Using a Database: Comparative Research with a Standard Sample http:// links are live

    E-Print Network [OSTI]

    White, Douglas R.

    Chapter 2 Using a Database: Comparative Research with a Standard Sample http:// links are live The first steps in testing hypotheses with a database are to search the codebook for variables relevant to the hypothesis, study how these variables are coded, and decide which variables to retrieve from the database

  2. Composting at HLS Brought to you by the Green Team and Green Living Program February 2011

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Composting at HLS Brought to you by the Green Team and Green Living Program February 2011 Americans.5% was composted. The environmental effect of NOT trashing this mass would be equivalent to removing 5.8 million cars from the road. (www.epa.gov)1 Compost Basics Why Compost? 1 Emissions avoidance from compost

  3. Composting 101 Prepared by the HLS Green Team and Green Living Program, updated February 2011

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Composting 101 Prepared by the HLS Green Team and Green Living Program, updated February 2011.5% was composted. The environmental effect of NOT trashing this mass would be equivalent to removing 5.8 million cars from the road. (www.epa.gov)1 What is Compost? Compost is the product of a biological process

  4. Environmental Factors and Breast Cancer Risk For millions of women whose lives

    E-Print Network [OSTI]

    Bezrukov, Sergey M.

    their health history, past environmental exposures, and lifestyle. The participants will be given yearly follow Gene The impact of family history on breast cancer risk suggests that genetic factors play an importantEnvironmental Factors and Breast Cancer Risk For millions of women whose lives have been affected

  5. hirty years ago,half the developing world lived in extreme poverty--

    E-Print Network [OSTI]

    Kammen, Daniel M.

    #12;T hirty years ago,half the developing world lived in extreme poverty-- today, a quarter.1 Now the billion mark this year for the first time in history.2 With so many still in poverty and hunger, growth and poverty alleviation remain the overarching priority for develop- ing countries. Climate change only makes

  6. LIVE CARS FOR USE IN CATFISH INDUSTRY Donald C. ,r nland, Rob rt L. ..rill,

    E-Print Network [OSTI]

    to deep water, or set adjacent to a well head for holding fish. Haul trucks can be scheduled more easily to loading sit s or shift d to saf areas for holding. When us ·d along Ith a haul Sf ine, pulling accurately because fish in properly staked live cars do not escape and can be harvested before truck arrives

  7. Faculty of Architecture and Planning Re-designing the World for 21st century Living

    E-Print Network [OSTI]

    Brownstone, Rob

    A Faculty of Architecture and Planning #12;1 Re-designing the World for 21st century Living A t Dalhousie's Faculty of Architecture and Planning we want to build a better world. And we are starting in our applied art and science we design and build vibrant, sustainable structures and communities -- places

  8. Car Sharing for the Twin Cities ! HOURCAR helps make it possible to live car-

    E-Print Network [OSTI]

    Minnesota, University of

    Car Sharing for the Twin Cities ! HOURCAR helps make it possible to live car- free or car-lite by filling in the "gaps" ! Car sharing reduces car ownership and saves its members thousands of dollars per year ! Car sharing "right-sizes" and makes transparent the costs of car usage #12;HOURCAR ! Transit

  9. Temporal and spatial variation in age-specific survival rates of a long-lived mammal,

    E-Print Network [OSTI]

    Aberdeen, University of

    . INTRODUCTION Understanding variability in survival patterns is funda- mental to life-history theory, wildlifeTemporal and spatial variation in age-specific survival rates of a long-lived mammal, the Hawaiian 8YJ, UK Estimates of variability in pinniped survival rates are generally based on observations

  10. The ultimate goal "Deciphering" the DNA nucleotide sequence of a living system*.

    E-Print Network [OSTI]

    Morante, Silvia

    The ultimate goal "Deciphering" the DNA nucleotide sequence of a living system*. Tools difficult macromolecule to analyse. Now it is possible to determine the nucleotide sequence at a rate of several hundred nucleotide a day. (August 2005: 1011 bases) #12;#12;#12;#12;#12;#12;#12;#12; = 0.283 #12

  11. Had my water gone bad? Family members have lived on our land

    E-Print Network [OSTI]

    Rhode Island, University of

    to find out. Hated to spend the money, but it gave me peace of mind. " " " We're protecting our family. We for more help. Some labs will do the sampling for you, right at your home. 4. After you collect your waterHad my water gone bad? Family members have lived on our land for generations. Never had a problem

  12. Discovery of benzene cation in a very long-lived excited electronic state Myung Soo Kima)

    E-Print Network [OSTI]

    Kim, Myung Soo

    Discovery of benzene cation in a very long-lived excited electronic state Myung Soo Kima) and Chan, University of Suwon, Suwon 440-600, Korea Received 19 May 2000; accepted 8 September 2000 Presence of benzene chemistry. © 2000 American Institute of Physics. S0021-9606 00 01745-1 I. INTRODUCTION The benzene molecular

  13. Method of freezing living cells and tissues with improved subsequent survival

    DOE Patents [OSTI]

    Senkan, Selim M. (Oak Ridge, TN); Hirsch, Gerald P. (Oak Ridge, TN)

    1980-01-01T23:59:59.000Z

    This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.

  14. A Non-Intrusive Monitoring System for Ambient Assisted Living Service Delivery

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    A Non-Intrusive Monitoring System for Ambient Assisted Living Service Delivery Willy All : Italy (2012)" #12;2 A Non-Intrusive Monitoring System for AAL Service Delivery Previous works from [6 is intrusive, and the massive deployment of sensors in the environment which implies a significant financial

  15. Predicting the fate of a living fossil: how will global warming affect sex determination

    E-Print Network [OSTI]

    Sheridan, Jennifer

    Predicting the fate of a living fossil: how will global warming affect sex determination, an unlikely response to global warming, as many oviparous species are nesting earlier as the climate warms. Keywords: climate change; global warming; temperature-dependent sex determination; reptile; Sphenodon 1

  16. Measurement of Local Viscoelasticity and Forces in Living Cells by Magnetic Tweezers

    E-Print Network [OSTI]

    Bausch, Andreas

    Measurement of Local Viscoelasticity and Forces in Living Cells by Magnetic Tweezers Andreas R measured the viscoelastic properties of the cytoplasm of J774 macrophages with a recently developed circuit, we measured the shear elastic modulus, the effective viscosities, and the strain relaxation time

  17. Study China Programme Live and learn in China for three weeks

    E-Print Network [OSTI]

    Oakley, Jeremy

    Study China Programme Live and learn in China for three weeks Study China is a unique learning, arts and business. About Study China Finance is provided by the UK government and managed Universities. Study China is in it's fourth year and has enabled over 1000 students to explore this fascinating

  18. Detecting Anomalous Sensor Events in Smart Home Data for Enhancing the Living Experience

    E-Print Network [OSTI]

    Cook, Diane J.

    Detecting Anomalous Sensor Events in Smart Home Data for Enhancing the Living Experience is on the rise and the place for smart home solutions is growing. One of the major concerns for smart home data captured from a smart home testbed. Introduction Smart homes are built by adding intelligent

  19. GREEN LIVING Replace incandencent and halogen light bulbs with LED and CLFs

    E-Print Network [OSTI]

    Thaxton, Christopher S.

    GREEN LIVING GUIDE ENERGY TRAVEL FOOD sustain yosef WATER Replace incandencent and halogen light for your laundry RESIST THAT SWITCH! Use natural light during the day, and no lights when you are gone USE MORE THAN YOU CAN EAT, reduce your waste stream DRINK FAIR TRADE COFFEE - Check out Conrad

  20. Incorporation of Short-Lived Be in a Calcium-Aluminum

    E-Print Network [OSTI]

    Incorporation of Short-Lived 10 Be in a Calcium-Aluminum­ Rich Inclusion from the Allende Meteorite Kevin D. McKeegan,1 * Marc Chaussidon,2 Franc¸ois Robert3 Enrichments in boron-10/boron-11 in a calcium-aluminum canonical abundance of aluminum-26 may still require seeding of the solar system by radioactive stellar

  1. Living on the Edge: Agriculture in Periurban Mexico City Andy Wanning

    E-Print Network [OSTI]

    Living on the Edge: Agriculture in Periurban Mexico City Andy Wanning Spring 2014 Capstone M.S. Sustainability Management Isla Urbana installs rainwater harvesting systems in and around Mexico City so that people aren't forced to get water via... Sistema Biobolsa installs biodigesters throughout Mexico so that

  2. Lecture Notes in Computer Science Towards a Living Software Development Process

    E-Print Network [OSTI]

    Lecture Notes in Computer Science Towards a Living Software Development Process based on Process Process for a certain enterprise and/or a certain project will usually integrate elements from a variety of existing process models, comprising generic standards as well as specific development methods. Besides that

  3. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

    SciTech Connect (OSTI)

    Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

    2013-04-01T23:59:59.000Z

    Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ? Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ? Tri-molecular complexes containing two scFvs and one molecule GK were formed. ? GK was detected using fluorescence lifetime-based FRET imaging. ? GK is monomeric and homogeneously distributed in several cancer cell lines. ? This technique may have many applications in live-cell imaging of endogenous proteins.

  4. Activity Based Costing

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1997-03-28T23:59:59.000Z

    Activity Based Costing (ABC) is method for developing cost estimates in which the project is subdivided into discrete, quantifiable activities or a work unit. This chapter outlines the Activity Based Costing method and discusses applicable uses of ABC.

  5. Assembly and actuation of nanomaterials using active biomolecules.

    SciTech Connect (OSTI)

    Spoerke, Erik David; Thayer, Gayle Echo; de Boer, Maarten Pieter; Bunker, Bruce Conrad; Liu, Jun; Corwin, Alex David; Gaudioso, Jennifer Marie; Sasaki, Darryl Yoshio; Boal, Andrew Kiskadden; Bachand, George David; Trent, Amanda M.; Bachand, Marlene; Rivera, Susan B.; Koch, Steven John

    2005-11-01T23:59:59.000Z

    The formation and functions of living materials and organisms are fundamentally different from those of synthetic materials and devices. Synthetic materials tend to have static structures, and are not capable of adapting to the functional needs of changing environments. In contrast, living systems utilize energy to create, heal, reconfigure, and dismantle materials in a dynamic, non-equilibrium fashion. The overall goal of the project was to organize and reconfigure functional assemblies of nanoparticles using strategies that mimic those found in living systems. Active assembly of nanostructures was studied using active biomolecules to drive the organization and assembly of nanocomposite materials. In this system, kinesin motor proteins and microtubules were used to direct the transport and interactions of nanoparticles at synthetic interfaces. In addition, the kinesin/microtubule transport system was used to actively assemble nanocomposite materials capable of storing significant elastic energy. Novel biophysical measurement tools were also developed for measuring the collective force generated by kinesin motor proteins, which will provide insight on the mechanical constraints of active assembly processes. Responsive reconfiguration of nanostructures was studied in terms of using active biomolecules to mediate the optical properties of quantum dot (QD) arrays through modulation of inter-particle spacing and associated energy transfer interaction. Design rules for kinesin-based transport of a wide range of nanoscale cargo (e.g., nanocrystal quantum dots, micron-sized polymer spheres) were developed. Three-dimensional microtubule organizing centers were assembled in which the polar orientation of the microtubules was controlled by a multi-staged assembly process. Overall, a number of enabling technologies were developed over the course of this project, and will drive the exploitation of energy-driven processes to regulate the assembly, disassembly, and dynamic reorganization of nanomaterials.

  6. LANL | Physics | Active Interrogation

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Physics Division activities in standoff active-interrogation for detecting terrorist nuclear devices, 2011 Detonation of a terrorist nuclear device in a major city would have...

  7. Active stewardship: sustainable future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Active stewardship: sustainable future Active stewardship: sustainable future Energy sustainability is a daunting task: How do we develop top-notch innovations with some of the...

  8. Light Activated Self-Propelled Colloids

    E-Print Network [OSTI]

    J. Palacci; S. Sacanna; S. -H. Kim; G. -R. Yi; D. J. Pine; P. M. Chaikin

    2014-10-27T23:59:59.000Z

    Light-activated self-propelled colloids are synthesized and their active motion is studied using optical microscopy. We propose a versatile route using different photoactive materials, and demonstrate a multiwavelength activation and propulsion. Thanks to the photoelectrochemical properties of two semiconductor materials (\\alpha Fe2 O3 and TiO2 ), a light with an energy higher than the bandgap triggers the reaction of decomposition of hydrogen peroxide and produces a chemical cloud around the particle. It induces a phoretic attraction with neighbouring colloids as well as an osmotic self- propulsion of the particle on the substrate. We use these mechanisms to form colloidal cargos as well as self-propelled particles where the light-activated component is embedded into a dielectric sphere. The particles are self-propelled along a direction otherwise randomized by thermal fluctuations, and exhibit a persistent random walk. For sufficient surface density, the particles spontaneously form "living crystals" which are mobile, break apart and reform. Steering the particle with an external magnetic field, we show that the formation of the dense phase results from the collisions heads-on of the particles. This effect is intrinsically non-equilibrium and a novel principle of organization for systems without detailed balance. Engineering families of particles self-propelled by different wavelength demonstrate a good understanding of both the physics and the chemistry behind the system and points to a general route for designing new families of self-propelled particles.

  9. Lively Infrastructure

    E-Print Network [OSTI]

    Amin, Ash

    2014-10-06T23:59:59.000Z

    and slack within and across the city’s infrastructural networks (Lahoud, 2010; Vale and Campanella, 2005; Batty, 2013). Importantly, this writing shows that there is nothing purely technical or mechanical about even the most digitised infrastructures... given to, and commanded by, building a house piece by piece when time and resource allow, the measures taken to pirate water and electricity, build sanitary pits, and make indoor or outdoor showers and kitchens, making a house into a home...

  10. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative1 First Use of Energy for All Purposes (Fuel and Nonfuel), 2002; Level: National5Sales for4,645 3,625 1,006 492 742EnergyOnItemResearch > The EnergyCenter (LMI-EFRC) - CenterLinks Berkeley LabTri-PartyEnergy

  11. LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Photography Protocol.docx revised 10/1/13 by JW Page 1 of 2

    E-Print Network [OSTI]

    Langerhans, Brian

    LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Photography Protocol.docx revised 10/1/13 by JW Page 1 of 2 Live Fish Photography Protocol Set up: Stand the white board used for preserved to the white board. Position camera so that fish area fills view. Make sure auto focus is on. Make camera

  12. Book review: Elizabeth E. Watson, 2009, Living Terraces in Ethiopia Konso Landscape, Culture & Development, Woodbridge and New York: James Currey

    E-Print Network [OSTI]

    Paris-Sud XI, Université de

    1 Book review: Elizabeth E. Watson, 2009, Living Terraces in Ethiopia ­ Konso Landscape, Culture'Ethiopie. Quotation: Demeulenaere, Elise. 2010. "Book review of Watson E. E., 2009, 'Living Terraces in Ethiopia territory in southern Ethiopia entirely shaped by agricultural terraces. For the author, most existing

  13. OPTICAL NANOSENSOR PARTICLES FOR DETECTION OF pH IN LIVING CELLS A. M. Scharff-Poulsena

    E-Print Network [OSTI]

    H sensitive dye and the reference dye permit intracellular pH measurements by fluorescence ratio imagingOPTICAL NANOSENSOR PARTICLES FOR DETECTION OF pH IN LIVING CELLS A. M. Scharff-Poulsena , H. Suna is limited by the lack of tools for measuring of metabolite levels in living cells with high spatial

  14. Global analysis of Fo rster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting

    E-Print Network [OSTI]

    van Stokkum, Ivo

    Global analysis of Fo¨ rster resonance energy transfer in live cells measured by fluorescence of Fo¨ rster resonance energy transfer (FRET) in live cells using the rise time of acceptor fluorescence those molecules that are involved in the energy-transfer process are monitored. This contrasts

  15. Roberta E. Rikli, C. Jessie Jones. (1997). Assessing Physical Performance in Independent Older Adults: Issues and Guidelines. Journal of Aging and Physical Activity, 5 (3).

    E-Print Network [OSTI]

    de Lijser, Peter

    Adults: Issues and Guidelines. Journal of Aging and Physical Activity, 5 (3). With the projected growth measure physical performance on a continuum across the wide range of functioning in the independent parameters associated with common activities of daily living. Additional tools are needed for measuring

  16. Nuclear Half-Lives for Alpha Radioactivity of Elements with 100 $\\leq$ Z $\\leq$ 130

    E-Print Network [OSTI]

    P. Roy Chowdhury; C. Samanta; D. N. Basu

    2008-02-29T23:59:59.000Z

    Theoretical estimates for the half lives of about 1700 isotopes of heavy elements with Z from 100 to 130 are tabulated using theoretical Q-values. The quantum mechanical tunneling probabilities are calculated within a WKB framework using microscopic nuclear potentials. The microscopic nucleus-nucleus potentials are obtained by folding the densities of interacting nuclei with a density dependent M3Y (DDM3Y) effective nucleon-nucleon interaction. The alpha-decay half lives calculated in this formalism using the experimental Q-values were found to be in good agreement over a wide range of experimental data spanning about twenty orders of magnitude. The theoretical Q-values used for the present calculations are extracted from three different mass estimates viz. Myers-Swiatecki [MS], Muntian-Hofmann-Patyk-Sobiczewski [M] and Koura-Tachibana-Uno-Yamada [KUTY].

  17. FRACTURE MECHANICS APPROACH TO ESTIMATE FATIGUE LIVES OF WELDED LAP-SHEAR SPECIMENS

    SciTech Connect (OSTI)

    Lam, P.; Michigan, J.

    2014-04-25T23:59:59.000Z

    A full range of stress intensity factor solutions for a kinked crack is developed as a function of weld width and the sheet thickness. When used with the associated main crack solutions (global stress intensity factors) in terms of the applied load and specimen geometry, the fatigue lives can be estimated for the laser-welded lap-shear specimens. The estimations are in good agreement with the experimental data. A classical solution for an infinitesimal kink is also employed in the approach. However, the life predictions tend to overestimate the actual fatigue lives. The traditional life estimations with the structural stress along with the experimental stress-fatigue life data (S-N curve) are also provided. In this case, the estimations only agree with the experimental data under higher load conditions.

  18. Shell model half-lives for r-process N=82 nuclei

    E-Print Network [OSTI]

    J. J. Cuenca-Garcia; G. Martinez-Pinedo; K. Langanke; F. Nowacki; I. N. Borzov

    2008-11-07T23:59:59.000Z

    We have performed shell-model calculations of the half-lives and neutron-branching probabilities of the r-process waiting point nuclei at the magic neutron number N=82. These new calculations use a larger model space than previous shell model studies and an improved residual interaction which is adjusted to recent spectroscopic data around A=130. Our shell-model results give a good account of all experimentally known half-lives and $Q_\\beta$-values for the N=82 r-process waiting point nuclei. Our half-life predictions for the N=82 nuclei with Z=42--46 agree well with recent estimates based in the energy-density functional method.

  19. Density waves in the shearing sheet IV. Interaction with a live dark halo

    E-Print Network [OSTI]

    B. Fuchs

    2004-03-01T23:59:59.000Z

    It is shown that if the self-gravitating shearing sheet, a model of a patch of a galactic disk, is embedded in a live dark halo, this has a strong effect on the dynamics of density waves in the sheet. I describe how the density waves and the halo interact via halo particles either on orbits in resonance with the wave or on non-resonant orbits. Contrary to expectation the presence of the halo leads to a very considerable enhancement of the amplitudes of the density waves in the shearing sheet. This effect appears to be the equivalent of the recently reported enhanced growth of bars in numerically simulated stellar disks embedded in live dark halos. Finally I discuss the transfer of linear momentum from a density wave in the sheet to the halo and show that it is mediated only by halo particles on resonant orbits.

  20. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Schot, Gijs, vander

    This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.

  1. Predicting carcass cutability of Rambouillet rams using live or carcass measures

    E-Print Network [OSTI]

    Snowder, Gary Douglas

    1980-01-01T23:59:59.000Z

    and hind weights were repeated three times. Muscling and finish scores were calculated from 8 to 12 judges. Repeatabiiity of these measures was computed by analysis of variance of between individuals and between measurements, within individuals (Becker...PREDICTING CARCASS CUTABILITY OF RAMBOUILLET RAMS USING LIVE OR CARCASS MEASURES A Thesis by GARY DOUGLAS SNOIVDER Submitted to the Graduate College of Texas ABM University in partial fulfillment of the requirement for the degree of MASTER...

  2. UTSA's SmartLiving Campus: A Real Time Approach to America's Future

    E-Print Network [OSTI]

    Gomez, J.

    2013-01-01T23:59:59.000Z

    UTSA SmartLiving CampusA Real-Time Approach to America’s Future ESL-KT-13-12-22 CATEE 2013: Clean Air Through Energy Efficiency Conference, San Antonio, Texas Dec. 16-18 Energy efficiency and conservation are two cost-effective, yet often...-12-22 CATEE 2013: Clean Air Through Energy Efficiency Conference, San Antonio, Texas Dec. 16-18 SOURCE: 2003 CBECS Nationwide: 98% of Bldgs San Antonio...

  3. Re-thinking residential mobility Linking lives through time and space

    E-Print Network [OSTI]

    Coulter, Rory; van Ham, Maarten; Findlay, Allan M.

    2015-03-16T23:59:59.000Z

    and support exchanges play a central role in the novel forms of resi- dential movement created by demographic and economic restructuring (Mulder, 2007). For example, many young people now move repeatedly in and out of the parental home during the protracted... ; Smart, 2011). Elder et al.’s (2003) principles of timing, life- span development and time and place stress the connections binding individual lives to structural conditions (Bailey, 2009). A useful starting point for conceptualizing these connec- tions...

  4. A review of "The Dead and the Living in Paris and London," by Vanessa Harding

    E-Print Network [OSTI]

    Edna Ruth Yahil

    2005-01-01T23:59:59.000Z

    . Vanessa Harding. The Dead and the Living in Paris and London, 1500-1600. Cambridge: Cambridge University Press, 2002. xvi + 343 pp. + 10 illus. + 2 maps. $65.00. Review by EDNA RUTH YAHIL, WASHINGTON STATE UNIVERSITY, SWISS CENTER. By 1670..., the populations of London and Paris exceeded 450,000, mak- ing these two of the largest cities in northern Europe. Both cities were capitals of centralizing states, and were represented by contemporaries as unified wholes despite being fractured judicially into a...

  5. Big-bang nucleosynthesis with a long-lived CHAMP including He4 spallation process

    E-Print Network [OSTI]

    Toshifumi Jittoh; Kazunori Kohri; Masafumi Koike; Joe Sato; Kenichi Sugai; Masato Yamanaka; Koichi Yazaki

    2012-09-10T23:59:59.000Z

    We propose helium-4 spallation processes induced by long-lived stau in supersymmetric standard models, and investigate an impact of the processes on light elements abundances. We show that, as long as the phase space of helium-4 spallation processes is open, they are more important than stau-catalyzed fusion and hence constrain the stau property. This talk is based on works (Jittoh et al., 2011).

  6. Predictions of Alpha Decay Half lives of Heavy and Superheavy Elements

    E-Print Network [OSTI]

    C. Samanta; P. Roy Chowdhury; D. N. Basu

    2007-05-28T23:59:59.000Z

    Theoretical estimates for the lifetimes of several isotopes of heavy elements with Z=102-120 are presented by calculating the quantum mechanical tunneling probability in a WKB framework and using microscopic nucleus-nucleus potential obtained by folding the densities of interacting nuclei with the DDM3Y effective nuclear interaction. The $\\alpha$-decay half lives calculated in this formalism using the experimental $Q$-values are in good agreement over a wide range of experimental data. Half lives are also calculated using $Q$-values extracted from two mass formulae. The Viola-Seaborg-Sobiczewski (VSS) estimates of $\\alpha$-decay half lives with the same $Q$-values are presented for comparison. The half life calculations are found to be quite sensitive to the choice of $Q$-values. Comparison with the experimental data delineates the inadequacies of older mass predictions in the domain of heavy and superheavy elements as compared to the newer one by Muntian-Hofmann-Patyk-Sobiczewski, and highlights necessity of a more accurate mass formula which can predict $Q$-values with even higher precision.

  7. Nanomechanical and topographical imaging of living cells by Atomic Force Microscopy with colloidal probes

    E-Print Network [OSTI]

    Luca Puricelli; Massimiliano Galluzzi; Carsten Schulte; Alessandro Podestà; Paolo Milani

    2015-02-05T23:59:59.000Z

    Atomic Force Microscopy (AFM) has a great potential as a tool to characterize mechanical and morphological properties of living cells; these properties have been shown to correlate with cells' fate and patho-physiological state in view of the development of novel early-diagnostic strategies. Although several reports have described experimental and technical approaches for the characterization of cell elasticity by means of AFM, a robust and commonly accepted methodology is still lacking. Here we show that micrometric spherical probes (also known as colloidal probes) are well suited for performing a combined topographic and mechanical analysis of living cells, with spatial resolution suitable for a complete and accurate mapping of cell morphological and elastic properties, and superior reliability and accuracy in the mechanical measurements with respect to conventional and widely used sharp AFM tips. We address a number of issues concerning the nanomechanical analysis, including the applicability of contact mechanical models and the impact of a constrained contact geometry on the measured elastic modulus (the finite-thickness effect). We have tested our protocol by imaging living PC12 and MDA-MB-231 cells, in order to demonstrate the importance of the correction of the finite-thickness effect and the change in cell elasticity induced by the action of a cytoskeleton-targeting drug.

  8. Search for a Neutral Long-Lived Particle Decaying to B-Jets

    SciTech Connect (OSTI)

    Johnson, Chad; /Columbia U.

    2009-04-01T23:59:59.000Z

    The existence of the Higgs boson is required by the Standard Model of particle physics, yet it has not been observed. The precise nature of the Higgs boson is unknown and the mechanism by which it interacts with known Standard Model particles is also not known. Long-lived, electrically neutral hadrons have recently been proposed in hidden-valley models and could constitute a pathway through which the Higgs boson communicates with the Standard Model. Such a scenario may provide a novel path to Higgs discovery at the Tevatron. This thesis describes a search for a neutral, long-lived particle produced in decays of Higgs bosons in p{bar p} collisions at a center-of-mass energy of {radical}s = 1.96 TeV, which decays to b-jets and lives long enough to travel at least 1.6 cm before decaying. This analysis uses 3.65 fb{sup -1} of data recorded with the Run II D0 detector at the Fermilab Tevatron collider from April 2002 to August of 2008. We perform a search for eight possible hidden-valley scenarios resulting from a Higgs decay. No significant excess over background is observed and cross-section limits are placed at 95% CL.

  9. Collective dynamics of active cytoskeletal networks

    E-Print Network [OSTI]

    Simone Köhler; Volker Schaller; Andreas R. Bausch

    2011-05-23T23:59:59.000Z

    Self organization mechanisms are essential for the cytoskeleton to adapt to the requirements of living cells. They rely on the intricate interplay of cytoskeletal filaments, crosslinking proteins and molecular motors. Here we present an in vitro minimal model system consisting of actin filaments, fascin and myosin-II filaments exhibiting pulsative collective long range dynamics. The reorganizations in the highly dynamic steady state of the active gel are characterized by alternating periods of runs and stalls resulting in a superdiffusive dynamics of the network's constituents. They are dominated by the complex competition of crosslinking molecules and motor filaments in the network: Collective dynamics are only observed if the relative strength of the binding of myosin-II filaments to the actin network allows exerting high enough forces to unbind actin/fascin crosslinks. The feedback between structure formation and dynamics can be resolved by combining these experiments with phenomenological simulations based on simple interaction rules.

  10. METHANE INCORPORATION BY PROCARYOTIC PHOTOSYNTHETIC MICROORGANISMS

    E-Print Network [OSTI]

    Norton, Charles J.; Kirk, Martha; Calvin, Melvin

    1970-01-01T23:59:59.000Z

    blue-green alga. Low-density nuclear material is abundant inobtained from the New England Nuclear Corpor- ation (NEN) inin C H The New England Nuclear Corporation was unable to

  11. Pentanol isomer synthesis in engineered microorganisms

    E-Print Network [OSTI]

    Cann, Anthony F.; Liao, James C.

    2010-01-01T23:59:59.000Z

    2-methyl-1-butanol and 3-methyl-1-butanol are a useful classMethyl-1-butanol . 3-Methyl-1-butanol . Biofuels 32 MJ/L for2007). In particular, 3-methyl-1-butanol (isoamyl alcohol)

  12. Microorganism genomics, compositions and methods related thereto

    DOE Patents [OSTI]

    Handelsman, Jo (Madison, WI); Goodman, Robert M. (Madison, WI); Rondon, Michelle R. (Madison, WI)

    2001-01-01T23:59:59.000Z

    The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

  13. Pentanol isomer synthesis in engineered microorganisms

    E-Print Network [OSTI]

    Cann, Anthony F.; Liao, James C.

    2010-01-01T23:59:59.000Z

    potential application as biofuels. They are found as natural3-Methyl-1-butanol . Biofuels 32 MJ/L for gasoline) and aabout microbial production of biofuels in general have been

  14. Biodegradation of Triclosan by Aerobic Microorganisms

    E-Print Network [OSTI]

    Lee, Do Gyun

    2012-10-19T23:59:59.000Z

    Triclosan, a synthetic antimicrobial agent, is an emerging environmental contaminant. Due to incomplete removal of triclosan by wastewater treatment plants, treated wastewater is one major source of environmental triclosan. Biodegradation...

  15. University Housing Departmental Performance Blueprint 2010-2011 Academic Year University Housing creates a sustainable living and learning community that promotes the academic success

    E-Print Network [OSTI]

    Almor, Amit

    Housing creates a sustainable living and learning community that promotes the academic success environments. #12;2 Contextual Statement Mission Statement: University Housing creates a sustainable living and personal development of students. Vision Sustainable Excellence: Great Today -- Greater Tomorrow Unit

  16. Bromine and iodine chemistry in a global chemistry-climate model: description and evaluation of very short-lived oceanic sources

    E-Print Network [OSTI]

    2012-01-01T23:59:59.000Z

    B. , Hebestreit, K. , and Platt, U. : Short-lived alkylA. , Winterlik, J. , and Platt, U. : Reactive and organic

  17. Active magnetic regenerator

    DOE Patents [OSTI]

    Barclay, John A. (Los Alamos, NM); Steyert, William A. (Los Alamos, NM)

    1982-01-01T23:59:59.000Z

    The disclosure is directed to an active magnetic regenerator apparatus and method. Brayton, Stirling, Ericsson, and Carnot cycles and the like may be utilized in an active magnetic regenerator to provide efficient refrigeration over relatively large temperature ranges.

  18. Moving past: probing the agency and affect of recordkeeping in individual and community lives in post-conflict Croatia

    E-Print Network [OSTI]

    Gilliland, AJ

    2014-01-01T23:59:59.000Z

    settlers in post- conflict Croatia, Intl J Hum Rights 17:Northern Ireland and Croatia, Fletcher F. World Aff 16:29-50Lives in Post-conflict Croatia --Manuscript Draft--

  19. Extraordinarily few organisms of a live recombinant BCG vaccine against tuberculosis induce maximal cell-mediated and protective immunity

    E-Print Network [OSTI]

    Horwitz, Marcus A; Harth, Günter; Dillon, Barbara Jane; Maslesa-Gali?, Sasa

    2006-01-01T23:59:59.000Z

    BCG vaccine by 0.7 logs in the lungs and 1.0 logs in the~10 live bacteria to the lungs of each animal, basedcounting primary lesions in the lungs of animals euthanized

  20. Development of High-throughput and Robust Microfluidic Live Cell Assay Platforms for Combination Drug and Toxin Screening

    E-Print Network [OSTI]

    Wang, Han

    2012-02-14T23:59:59.000Z

    the labor-intensive and time-consuming limitations, they are too costly for general users. Microfluidic live cell screening platforms can allow precise control of cell culture microenvironments by applying accurate doses of biomolecular mixtures...

  1. Study on lipid droplet dynamics in live cells and fluidity changes in model bacterial membranes using optical microscopy techniques 

    E-Print Network [OSTI]

    Wong, Christine Shiang Yee

    2014-06-28T23:59:59.000Z

    In this thesis optical microscopy techniques are used to consider aspects of viral and bacterial infections. In part 1, the physical effects of cytomegalovirus on lipid droplet dynamics in live cells are studied; in part ...

  2. THE HEALTH BENEFITS OF CAFFEINE Everything you need to know to think faster, exercise harder, and live longer

    E-Print Network [OSTI]

    Lockery, Shawn

    to let you know it's time to turn off Conan and recharge your batteries. Adenosine accomplishes, and live longer By Lauren Russell Griffin, Photo Illustrations by Eddie Guy By now you'd think scientists

  3. Growing Up in Scotland: Year 2 - Results from the second year of a study following the lives of Scotland's children 

    E-Print Network [OSTI]

    Bradshaw, Paul; Cunningham-Burley, Sarah; Dobie, Fiona; MacGregor, Andy; Marryat, Louise; Ormston, Rachel; Wasoff, Fran

    2008-02-18T23:59:59.000Z

    The Growing Up in Scotland study (GUS) is an important longitudinal research project aimed at tracking the lives of a cohort of Scottish children from the early years, through childhood and beyond. Its principal aim is ...

  4. as Ag Biotech Lived Up to Its Promise? (and what should the scientific community do about it?)

    E-Print Network [OSTI]

    Clark, E. Ann

    H as Ag Biotech Lived Up to Its Promise? (and what should the scientific community do about it?) E that conventional plant breeding could do - and better. He also had a section entitled Standing Up

  5. Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging

    E-Print Network [OSTI]

    White, Katharine Alice

    2012-01-01T23:59:59.000Z

    Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. ...

  6. Energy Conservation and Comfort of Heat Pump Desiccant Air Conditioning System in Actual Living Space in Summer

    E-Print Network [OSTI]

    Miyashita, Yasushi

    Energy Conservation and Comfort of Heat Pump Desiccant Air Conditioning System in Actual Living and total heat exchanger in terms of both energy conservation and thermal comfort in summer. 1. COP

  7. The Effects of Mandatory Country-of-Origin Labeling on Canadian/U.S. Live Hog and Feeder Pig Trade

    E-Print Network [OSTI]

    Thevenaz, Shad Arthur Michel

    2012-02-14T23:59:59.000Z

    The final implementation of Mandatory Country-of-Origin Labeling has caused some U.S. packing plants and finishing operations to discontinue using Canadian live hogs and feeder pigs in their operations thereby reducing trade. Using a system...

  8. Understanding the Triaging and Fixing Processes of Long Lived Bugs Ripon K. Saha, Sarfraz Khurshid, Dewayne E. Perry

    E-Print Network [OSTI]

    Perry, Dewayne E.

    Understanding the Triaging and Fixing Processes of Long Lived Bugs Ripon K. Saha, Sarfraz Khurshid- velopers/managers triage bugs carefully and schedule Email addresses: ripon@utexas.edu (Ripon K. Saha

  9. An investigation into the role of genetics in the tolerance of Texas live oaks to Ceratocystis fagacearum

    E-Print Network [OSTI]

    Gray, Myron Crowley

    2009-05-15T23:59:59.000Z

    The fungus Ceratocystis fagacearum (Bretz) Hunt causes the vascular disease of oak wilt and has been decimating live oaks (Quercus virginiana Mill. and Quercus fusiformis Small.) and red oaks (Quercus texana Small and Quercus marilandica Muenchh...

  10. +++ CIVIL WAR IN SOUTH SUDAN CLAIMS THOUSANDS OF LIVES +++ NUMBER OF DIVORCES IN IRAN TRIPLES +++ AT LEAST 15 DEAD

    E-Print Network [OSTI]

    +++ CIVIL WAR IN SOUTH SUDAN CLAIMS THOUSANDS OF LIVES +++ NUMBER OF DIVORCES IN IRAN TRIPLES of South Sudan, the newest member of the international community of sovereign states, shows. Researchers

  11. Collective spaces : a study in the conversion of storage to living spaces in City of Industry, California

    E-Print Network [OSTI]

    Su, Tony H. (Tony Hsuan Ching)

    2005-01-01T23:59:59.000Z

    A research and design study was instigated to rethink the phenomenon of storage in relation to contemporary living spheres. Although few historical traces of personal storage remain, the study of the evolution of commercial ...

  12. An investigation into the role of genetics in the tolerance of Texas live oaks to Ceratocystis fagacearum 

    E-Print Network [OSTI]

    Gray, Myron Crowley

    2009-05-15T23:59:59.000Z

    The fungus Ceratocystis fagacearum (Bretz) Hunt causes the vascular disease of oak wilt and has been decimating live oaks (Quercus virginiana Mill. and Quercus fusiformis Small.) and red oaks (Quercus texana Small and Quercus marilandica Muenchh...

  13. A Hierarchical Characterization of a Live Streaming Media Workload Eveline Veloso Virgilio Almeida Wagner Meira Azer Bestavros Shudong Jin

    E-Print Network [OSTI]

    A Hierarchical Characterization of a Live Streaming Media Workload£ Eveline Veloso Virg´ilio Almeida Wagner Meira Azer Bestavros Shudong Jin eveline@dcc.ufmg.br virgilio@dcc.ufmg.br meira

  14. A Hierarchical Characterization of a Live Streaming Media Workload Eveline Veloso Virg lio Almeida Wagner Meira Azer Bestavros Shudong Jin

    E-Print Network [OSTI]

    A Hierarchical Characterization of a Live Streaming Media Workload #3; Eveline Veloso Virgâ?? �lio Almeida Wagner Meira Azer Bestavros Shudong Jin eveline@dcc.ufmg.br virgilio@dcc.ufmg.br meira

  15. Developing new optical imaging techniques for single particle and molecule tracking in live cells

    SciTech Connect (OSTI)

    Sun, Wei

    2010-12-15T23:59:59.000Z

    Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian cells. New rotational information was obtained: (1) during endocytosis, cargoes lost their rotation freedom at the late stage of internalization; (2) cargoes performed train-like motion when they were transported along the microtubule network by motor proteins inside live cells; (3) During the pause stage of fast axonal transport, cargoes were still bound to the microtubule tracks by motor proteins. Total internal reflection fluorescence microscopy (TIRFM) is another non-invasive and far-field optical imaging technique. Because of its near-field illumination mechanism, TIRFM has better axial resolution than epi-fluorescence microscopy and confocal microscopy. In this work, an auto-calibrated, prism type, angle-scanning TIRFM instrument was built. The incident angle can range from subcritical angles to nearly 90{sup o}, with an angle interval less than 0.2{sup o}. The angle precision of the new instrument was demonstrated through the finding of the surface plasmon resonance (SPR) angle of metal film coated glass slide. The new instrument improved significantly the precision in determining the axial position. As a result, the best obtained axial resolution was {approx} 8 nm, which is better than current existing instruments similar in function. The instrument was further modified to function as a pseudo TIRF microscope. The illumination depth can be controlled by changing the incident angle of the excitation laser beam or adjusting the horizontal position of the illumination laser spot on the prism top surface. With the new technique, i.e., variable-illumination-depth pseudo TIRF microscopy, the whole cell body from bottom to top was scanned.

  16. A retrospective study on changes in residents' physical activities, social 

    E-Print Network [OSTI]

    Zhu, Xuemei; Yu, Chia-Yuan; Lee, Chanam; Lu, Zhipeng; Mann, George

    2014-01-01T23:59:59.000Z

    , walkable communities typically feature? This paper is being submitted for the Active Living Re ? Corresponding author. E-mail addresses: xzhu@arch.tamu.edu (X. Zhu), yu17 clee@arch.tamu.edu (C. Lee), zlu@arch.tamu.edu (Z. Lu), m http://dx.doi.org/10.1016/j...,?, Chia-Yuan Yu b, Chanam Lee b, Zhipen a Department of Architecture, Center for Health Systems & Design, Texas A&M University, 313 b Department of Landscape Architecture and Urban Planning, Center for Health Systems & Dessearch Supplement. 891@neo...

  17. Animals In Synchrotrons: Overcoming Challenges For High-Resolution, Live, Small-Animal Imaging

    SciTech Connect (OSTI)

    Donnelley, Martin [Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, South Australia (Australia); Parsons, David [Respiratory and Sleep Medicine, Women's and Children's Hospital, Adelaide, South Australia (Australia); Women's and Children's Health Research Institute, Adelaide, South Australia (Australia); Department of Paediatrics and Reproductive Health, University of Adelaide, South Australia (Australia); Department of Paediatircs Centre for Stem Cell Research, University of Adelaide, South Australia (Australia); Morgan, Kaye [School of Physics, Monash University, Melbourne, Victoria (Australia); Siu, Karen [School of Physics, Monash University, Melbourne, Victoria (Australia); Monash Centre for Synchrotron Science, Monash University, Melbourne, Victoria (Australia)

    2010-07-23T23:59:59.000Z

    Physiological studies in small animals can be complicated, but the complexity is increased dramatically when performing live-animal synchrotron X-ray imaging studies. Our group has extensive experience in high-resolution live-animal imaging at the Japanese SPring-8 synchrotron, primarily examining airways in two-dimensions. These experiments normally image an area of 1.8 mmx1.2 mm at a pixel resolution of 0.45 {mu}m and are performed with live, intact, anaesthetized mice.There are unique challenges in this experimental setting. Importantly, experiments must be performed in an isolated imaging hutch not specifically designed for small-animal imaging. This requires equipment adapted to remotely monitor animals, maintain their anesthesia, and deliver test substances while collecting images. The horizontal synchrotron X-ray beam has a fixed location and orientation that limits experimental flexibility. The extremely high resolution makes locating anatomical regions-of-interest slow and can result in a high radiation dose, and at this level of magnification small animal movements produce motion-artifacts that can render acquired images unusable. Here we describe our experimental techniques and how we have overcome several challenges involved in performing live mouse synchrotron imaging.Experiments have tested different mouse strains, with hairless strains minimizing overlying skin and hair artifacts. Different anesthetics have also be trialed due to the limited choices available at SPring-8. Tracheal-intubation methods have been refined and controlled-ventilation is now possible using a specialized small-animal ventilator. With appropriate animal restraint and respiratory-gating, motion-artifacts have been minimized. The animal orientation (supine vs. head-high) also appears to affect animal physiology, and can alter image quality. Our techniques and image quality at SPring-8 have dramatically improved and in the near future we plan to translate this experience to the Imaging and Medical Beamline at the Australian Synchrotron.Overcoming these challenges has permitted increasingly sophisticated imaging of animals with synchrotron X-rays, and we expect a bright future for these techniques.

  18. Phase Transition of Trapped Nuclear Exciton of Long-lived Rhodium Mossbauer States

    E-Print Network [OSTI]

    Xia, Bing

    2007-01-01T23:59:59.000Z

    We report two experimental observations from the long-lived rhodium Mossbauer emissions by means of the time- and energy-resolved spectroscopy. Firstly, phase transitions in time evolution indicated by characteristic spectral profiles reveal the aggregate exciton orderings of inverted nuclei at room temperature. Depending on the exciton density in polycrystal, six different phases featured by multiple ionizations are identified. Secondly, broad-band background emissions are discovered and are believed to be ascribed to branching channels of gamma radiation. More than half gamma radiation goes to these anisotropic channels. Cascade scheme to catalyze the E3 multipolar nuclear transition is thus suggested.

  19. Search for long-lived isomeric states in neutron-deficient thorium isotopes

    SciTech Connect (OSTI)

    Lachner, J.; Dillmann, I.; Faestermann, T.; Korschinek, G.; Poutivtsev, M.; Rugel, G. [Physik Department E12 and E15, Technische Universitaet Muenchen, D-85748 Garching (Germany)

    2008-12-15T23:59:59.000Z

    The discovery of naturally occurring long-lived isomeric states (t{sub 1/2}>10{sup 8} yr) in the neutron-deficient isotopes {sup 211,213,217,218}Th[A. Marinov et al., Phys. Rev. C 76, 021303(R) (2007)] was reexamined using accelerator mass spectrometry (AMS). Because AMS does not suffer from molecular isobaric background in the detection system, it is an extremely sensitive technique. Despite our up to two orders of magnitude higher sensitivity we cannot confirm the discoveries of neutron-deficient thorium isotopes and provide upper limits for their abundances.

  20. Search for long-lived isomeric states in neutron-deficient thorium isotopes

    E-Print Network [OSTI]

    J. Lachner; I. Dillmann; T. Faestermann; G. Korschinek; M. Poutivtsev; G. Rugel

    2009-07-01T23:59:59.000Z

    The discovery of naturally occurring long-lived isomeric states (t_1/2 > 10^8 yr) in the neutron-deficient isotopes 211,213,217,218Th [A. Marinov et al., Phys. Rev. C 76, 021303(R) (2007)] was reexamined using accelerator mass spectrometry (AMS). Because AMS does not suffer from molecular isobaric background in the detection system, it is an extremely sensitive technique. Despite our up to two orders of magnitude higher sensitivity we cannot confirm the discoveries of neutron-deficient thorium isotopes and provide upper limits for their abundances.