National Library of Energy BETA

Sample records for microorganisms living active

  1. Microorganism immobilization

    DOE Patents [OSTI]

    Compere, Alicia L. (Knoxville, TN); Griffith, William L. (Oak Ridge, TN)

    1981-01-01

    Live metabolically active microorganisms are immobilized on a solid support by contacting particles of aggregate material with a water dispersible polyelectrolyte such as gelatin, crosslinking the polyelectrolyte by reacting it with a crosslinking agent such as glutaraldehyde to provide a crosslinked coating on the particles of aggregate material, contacting the coated particles with live microorganisms and incubating the microorganisms in contact with the crosslinked coating to provide a coating of metabolically active microorganisms. The immobilized microorganisms have continued growth and reproduction functions.

  2. Response of Free-Living Nitrogen-Fixing Microorganisms to Land Use Change in the Amazon Rainforest

    E-Print Network [OSTI]

    Bohannan, Brendan

    ) of this greenhouse gas annually (1, 2). To absorb this enormous amount of CO2, a sig- nificant input of nitrogen (NResponse of Free-Living Nitrogen-Fixing Microorganisms to Land Use Change in the Amazon Rainforest-living nitrogen-fixing microorganisms (di- azotrophs) respond to deforestation in the Amazon rainforest, using

  3. Bursts of Active Transport in Living Cells

    E-Print Network [OSTI]

    Bo Wang; James Kuo; Steve Granick

    2013-07-07

    We scrutinize the temporally-resolved speed of active cargo transport in living cells, and show intermittent bursting motions. These nonlinear fluctuations follow a scaling law over several decades of time and space, the statistical regularities displaying a time-averaged shape that we interpret to reflect stress buildup followed by rapid release. The power law of scaling is the same as seen in driven jammed colloids, granular, and magnetic systems. The implied regulation of active transport with environmental obstruction extends the classical notion of molecular crowding.

  4. Microorganisms for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-09-30

    Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

  5. Probing active forces via a fluctuation-dissipation relation: Application to living cells

    E-Print Network [OSTI]

    Pierre Bohec; François Gallet; Christian Maes; Soghra Safaverdi; Paolo Visco; Frédéric Van Wijland

    2013-06-24

    We derive a new fluctuation-dissipation relation for non-equilibrium systems with long-term memory. We show how this relation allows one to access new experimental information regarding active forces in living cells that cannot otherwise be accessed. For a silica bead attached to the wall of a living cell, we identify a crossover time between thermally controlled fluctuations and those produced by the active forces. We show that the probe position is eventually slaved to the underlying random drive produced by the so-called active forces.

  6. Measuring 3D Arm Movements for Activities of Daily Living Thomas Haslwanter

    E-Print Network [OSTI]

    Haslwanter, Thomas

    - 1 - Measuring 3D Arm Movements for Activities of Daily Living Thomas Haslwanter Upper Austria in everyday life show a large variability between different, healthy subjects. In order to find reference arm movements for monitoring the progress of rehabilitation after stroke, we investigated the similarity of arm

  7. Active mechanics reveal molecular-scale force kinetics in living oocytes

    E-Print Network [OSTI]

    Ahmed, Wylie W; Almonacid, Maria; Bussonnier, Matthias; Verlhac, Marie-Helene; Gov, Nir S; Visco, Paolo; van Wijland, Frederic; Betz, Timo

    2015-01-01

    Unlike traditional materials, living cells actively generate forces at the molecular scale that change their structure and mechanical properties. This nonequilibrium activity is essential for cellular function, and drives processes such as cell division. Single molecule studies have uncovered the detailed force kinetics of isolated motor proteins in-vitro, however their behavior in-vivo has been elusive due to the complex environment inside the cell. Here, we quantify active force generation in living oocytes using in-vivo optical trapping and laser interferometry of endogenous vesicles. We integrate an experimental and theoretical framework to connect mesoscopic measurements of nonequilibrium properties to the underlying molecular-scale force kinetics. Our results show that force generation by myosin-V drives the cytoplasmic-skeleton out-of-equilibrium (at frequencies below 300 Hz) and actively softens the environment. In vivo myosin-V activity generates a force of $F \\sim 0.4$ pN, with a power-stroke of len...

  8. Cellulolytic Microorganisms from Thermal Environments

    SciTech Connect (OSTI)

    Vishnivetskaya, Tatiana A [ORNL; Raman, Babu [ORNL; Phelps, Tommy Joe [ORNL; Podar, Mircea [ORNL; Elkins, James G [ORNL

    2012-01-01

    Thermal, anaerobic environments rich in decaying plant material are a potential source of novel cellulolytic bacteria. Samples collected from geothermal aquifers in the Yellowstone National Park (YNP) were used to select for cellulolytic thermophiles. Laboratory enrichments on dilute-acid pretreated plant biomass (switchgrass, Populus), and crystalline cellulose (Avicel) resulted in the isolation of 247 environmental clones. The majority of individual clones were affiliated with the cellulolytic bacteria of phylum Firmicutes, followed by xylanolytic and saccharolytic members of the phylum Dictyoglomi. Among the Firmicutes, the clones were affiliated with the genera Caldicellulosiruptor (54.4%), Caloramator (11.5%), Thermoanaerobacter (8.8%), Thermovenabulum (4.1%), and Clostridium (2.0%). From established anaerobic thermophilic enrichments a total of 81 single strains of the genera Caldicellulosiruptor (57%) and Thermoanaerobacter (43%) were isolated. With continuous flow enrichment on Avicel, increases in the relative abundance of Caloramator sp. was observed over clones detected from the Caldicellulosiruptor. Complex communities of interacting microorganisms bring about cellulose decomposition in nature, therefore using up-to-date approaches may yield novel cellulolytic microorganisms with high activity and a rapid rate of biomass conversion to biofuels.

  9. Nearby Outdoor Environmental Support of Older Adults' Yard Activities, Neighborhood Walking and Independent Living in the Community 

    E-Print Network [OSTI]

    Wang, Zhe

    2011-02-22

    Aging is a global phenomenon. Ways to sustain older adults' aging-in-place in the 'community at-large' (defined as traditional communities where most people live) have been overlooked. Consciously engaging in physical activity helps older adults...

  10. Engineered microorganisms capable of producing target compounds under anaerobic conditions

    DOE Patents [OSTI]

    Buelter, Thomas (Denver, CO); Meinhold, Peter (Denver, CO); Feldman, Reid M. Renny (San Francisco, CA); Hawkins, Andrew C. (Parker, CO); Urano, Jun (Irvine, CA); Bastian, Sabine (Pasadena, CA); Arnold, Frances (La Canada, CA)

    2012-01-17

    The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

  11. Proteolysis in hyperthermophilic microorganisms

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Ward, Donald E.; Shockley, Keith R.; Chang, Lara S.; Levy, Ryan D.; Michel, Joshua K.; Conners, Shannon B.; Kelly, Robert M.

    2002-01-01

    Proteases are found in every cell, where they recognize and break down unneeded or abnormal polypeptides or peptide-based nutrients within or outside the cell. Genome sequence data can be used to compare proteolytic enzyme inventories of different organisms as they relate to physiological needs for protein modification and hydrolysis. In this review, we exploit genome sequence data to compare hyperthermophilic microorganisms from the euryarchaeotal genusPyrococcus, the crenarchaeoteSulfolobus solfataricus, and the bacteriumThermotoga maritima. An overview of the proteases in these organisms is given based on those proteases that have been characterized and on putative proteases that have been identified from genomicmore »sequences, but have yet to be characterized. The analysis revealed both similarities and differences in the mechanisms utilized for proteolysis by each of these hyperthermophiles and indicated how these mechanisms relate to proteolysis in less thermophilic cells and organisms.« less

  12. Fast-Neutron Activation of Long-Lived Nuclides in Natural Pb

    E-Print Network [OSTI]

    V. E. Guiseppe; S. R. Elliott; N. E. Fields; D. Hixon

    2012-09-20

    We measured the production of the long-lived nuclides Bi-207, Pb-202, and Hg-194 in a sample of natural Pb due to high-energy neutron interactions using a neutron beam at the Los Alamos Neutron Science Center. The activated sample was counted by a HPGe detector to measure the amount of radioactive nuclides present. These nuclides are critical in understanding potential backgrounds in low background experiments utilizing large amounts of Pb shielding due to cosmogenic neutron interactions in the Pb while residing on the Earth's surface. By scaling the LANSCE neutron flux to a cosmic neutron flux, we measure the sea level cosmic ray production rates of 8.0 +/- 1.3 atoms/kg/day of Hg-194, 120 +/- 25 atoms/kg/day Pb-202, and 0.17 +/- 0.04 atoms/kg/day Bi-207.

  13. University of Calgary Active Living Programs for Children and YouthUniversity of Calgary Active Living Programs for Children & Youth Register Today! | (403) 220-7749 | ucalgary.ca/activeliving/kids

    E-Print Network [OSTI]

    Calgary, University of

    Living Programs for Children & Youth Register Today! | (403) 220-7749 | ucalgary.ca/activeliving/kids FALL/SUMMER 2015 #12;REGISTER | UCALGARY.CA/ACTIVELIVING/KIDS | (403) 220-77492 Registration Online, or to register by phone, please contact Client Services: (403) 220-7749 active@ucalgary.ca Waiver Form

  14. Contribution of microorganisms to corrosion

    SciTech Connect (OSTI)

    Thorp, K.E.G.; Crasto, A.S. [Univ. of Dayton Research Inst., OH (United States); Gu, J.D.; Mitchell, R. [Harvard Univ., Cambridge, MA (United States). Div. of Applied Sciences

    1997-08-01

    Current metal primers utilized by the US Air Force contain chromates to inhibit corrosion of the underlying metal. These chromates are both highly toxic and carcinogenic and pose a severe health risk to personnel involved in their application, stripping and disposal. Environmentally-friendly primers with chromate replacements have historically performed poorly with respect to corrosion inhibition. The purpose of this study was to investigate the interaction of chromates with microorganisms in an environment not traditionally associated with biologically-enhanced corrosion to determine if corrosion inhibition by a chromate pigment is, in part, through its action as a biocide. Inoculation of panels which had been coated with a nonchromated primer prior to salt fog exposure and storage in humid conditions resulted in a significant growth of filiform corrosion around a scribe mark. The presence of chromate in the primer severely limited the formation of this corrosion. Likewise, in the absence of the inoculation procedure, the extent of corrosion was strongly diminished. These results suggest that the chromate may be acting as a biocide to limit corrosion which is enhanced by the presence of biological activity.

  15. Digital holographic imaging of microorganisms

    E-Print Network [OSTI]

    Wolf, Michael Trevor

    2006-01-01

    Imaging aquatic microorganisms in 3D space is of interest to biologists and ocean scientists seeking to understand the behavior of these organisms in their natural environments. In this research, digital holographic imaging ...

  16. Journal of Food,Agriculture & Environment, Vol.7 (2),April 2009 103 Antimicrobial activity against pathogenic microorganisms by extracts from herbal

    E-Print Network [OSTI]

    Khalil, Amjad

    Journal of Food,Agriculture & Environment, Vol.7 (2),April 2009 103 Antimicrobial activity against-fungal activity. www.world-food.netJournal of Food, Agriculture & Environment Vol.7 (2) : 103-106. 2009

  17. Organic acid-tolerant microorganisms and uses thereof for producing organic acids

    DOE Patents [OSTI]

    Pfleger, Brian Frederick; Begemann, Matthew Brett

    2014-05-06

    Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

  18. THE ASSOCIATION BETWEEN PHYSICAL ACTIVITY, COGNITIVE FUNCTION, AND PERFORMANCE OF ACTIVITIES OF DAILY LIVING IN PATIENTS WITH EARLY ALZHEIMER'S DISEASE

    E-Print Network [OSTI]

    Jaber, Ala'a Fayez

    2010-05-31

    psychometric battery. Structural MRI was obtained using a Siemens 3.0 Tesla Allegra MRI scanner at the Hoglund Brain Imaging Center located adjacent to the Center on Aging. The outcome measures included: 1) Physical Activity Scale in the Elderly (PASE) and a... modified Community Healthy Activities Model Program for Seniors (CHAMPS): Both questionnaires were used to assess physical activity levels; 2) Physical Performance Test (PPT): This short battery of timed physical tasks was used as a measure of physical...

  19. The Economic Benefits of Built Environment Supportive of Active Living in Dallas Tax Increment Financing Districts 

    E-Print Network [OSTI]

    Xu, Minjie

    2015-08-11

    of the most active and successfully practiced mechanisms to implement smart growth principles. This dissertation consists of three studies in the city of Dallas to examine different outcomes related to the study goals. In Chapter 2, I employed a quasi... collaboration in development decisions. Since then, smart growth has rapidly gained its popularity. Proponents of smart growth include urban and transportation planners, public health professions, environmentalists, and central city advocates, among others...

  20. Emerging and opportunistic diseases are caused by a microorganism invading a new

    E-Print Network [OSTI]

    Gomulkiewicz, Richard

    Emerging and opportunistic diseases are caused by a microorganism invading a new habitat, either meningitis). Often, the pathogen can live for many generations in the new habitat and, as natural selection be applied to most bacterial pathogens and suggest a phylogeny-based method to search for genes undergoing

  1. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    For Users Live Status Queue Look Classic Queue Look Scheduled Outages Outage Log Science Gateway Status Login Node Status Filesystem Status My NERSC Move to CRT Getting Started...

  2. Microfluidic passive samplers for in situ collection of live aquatic protists

    E-Print Network [OSTI]

    Shor, Leslie McCabe

    Microfluidic passive samplers for in situ collection of live aquatic protists Grant M. Bouchillon of microfluidic passive samplers for the collection of live protists from natural aquatic habitats. Microfluidic microfluidic observation galleries. In field experiments, live protists and other microorganisms were collected

  3. Quantum superposition, entanglement, and state teleportation of a microorganism on an electromechanical oscillator

    E-Print Network [OSTI]

    Li, Tongcang

    2015-01-01

    Schr\\"odinger's thought experiment to prepare a cat in a superposition of both alive and dead states reveals profound consequences of quantum mechanics and has attracted enormous interests. Here we propose a straightforward method to create quantum superposition states of a living microorganism by putting a small bacterium on top of an electromechanical oscillator. Our proposal is based on recent developments that the center-of-mass oscillation of a 15-$\\mu$m-diameter aluminium membrane has been cooled to its quantum ground state [Nature 475, 359 (2011)], and entangled with a microwave field [Science, 342, 710 (2013)]. A microorganism with a mass much smaller than the mass of the electromechanical membrane will not significantly affect the quality factor of the membrane and can be cooled to the quantum ground state together with the membrane. Quantum superposition and teleportation of its center-of-mass motion state can be realized with the help of superconducting microwave circuits. More importantly, the int...

  4. Recent activities for ?-decay half-lives and ?-delayed neutron emission of very neutron-rich isotopes

    SciTech Connect (OSTI)

    Dillmann, Iris; Abriola, Daniel; Singh, Balraj

    2014-05-02

    Beta-delayed neutron (?n) emitters play an important, two-fold role in the stellar nucleosynthesis of heavy elements in the 'rapid neutron-capture process' (r process). On one hand they lead to a detour of the material ?-decaying back to stability. On the other hand, the released neutrons increase the neutron-to-seed ratio, and are re-captured during the freeze-out phase and thus influence the final solar r-abundance curve. A large fraction of the isotopes inside the r-process reaction path are not yet experimentally accessible and are located in the (experimental) 'Terra Incognita'. With the next generation of fragmentation and ISOL facilities presently being built or already in operation, one of the main motivation of all projects is the investigation of these very neutron-rich isotopes. A short overview of one of the planned programs to measure ?n-emitters at the limits of the presently know isotopes, the BRIKEN campaign (Beta delayed neutron emission measurements at RIKEN) will be given. Presently, about 600 ?-delayed one-neutron emitters are accessible, but only for a third of them experimental data are available. Reaching more neutron-rich isotopes means also that multiple neutron-emission becomes the dominant decay mechanism. About 460 ?-delayed two-, three-or four-neutron emitters are identified up to now but for only 30 of them experimental data about the neutron branching ratios are available, most of them in the light mass region below A=30. The International Atomic and Energy Agency (IAEA) has identified the urgency and picked up this topic recently in a 'Coordinated Research Project' on a 'Reference Database for Beta-Delayed Neutron Emission Data'. This project will review, compile, and evaluate the existing data for neutron-branching ratios and half-lives of ?-delayed neutron emitters and help to ensure a reliable database for the future discoveries of new isotopes and help to constrain astrophysical and theoretical models.

  5. Chemosensing in microorganisms to practical biosensors

    E-Print Network [OSTI]

    Surya K. Ghosh; Tapanendu Kundu; Anirban Sain

    2012-10-25

    Microorganisms like bacteria can sense concentration of chemo-attractants in its medium very accurately. They achieve this through interaction between the receptors on their cell surface and the chemo-attractant molecules (like sugar). But the physical processes like diffusion set some limits on the accuracy of detection which was discussed by Berg and Purcell in the late seventies. We have a re-look at their work in order to assess what insight it may offer towards making efficient, practical biosensors. We model the functioning of a typical biosensor as a reaction-diffusion process in a confined geometry. Using available data first we characterize the system by estimating the kinetic constants for the binding/unbinding reactions between the chemo-attractants and the receptors. Then we compute the binding flux for this system which Berg and Purcell had discussed. But unlike in microorganisms where the interval between successive measurements determines the efficiency of the nutrient searching process, it turns out that biosensors depend on long time properties like signal saturation time which we study in detail. We also develop a mean field description of the kinetics of the system.

  6. Where the active galaxies live: a panchromatic view of radio-AGN in the AKARI-NEP field

    E-Print Network [OSTI]

    Karouzos, Marios; Trichas, Markos

    2013-01-01

    We study the host galaxy properties of radio sources in the AKARI-North Ecliptic Pole (NEP) field, using an ensemble of multi-wavelength datasets. We identify both radio-loud and radio-quiet AGN and study their host galaxy properties by means of SED fitting. We investigate the relative importance of nuclear and star-formation activity in radio-AGN and assess the role of radio-AGN as efficient quenchers of star-formation in their host galaxies.

  7. Storing data encoded DNA in living organisms

    DOE Patents [OSTI]

    Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)

    2006-06-06

    Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.

  8. Sensory Transduction in Microorganisms 2008 Gordon Research Conference (January 2008)

    SciTech Connect (OSTI)

    Ann M. Stock

    2009-04-08

    Research into the mechanisms involved in the sensing and responses of microorganisms to changes in their environments is currently very active in a large number of laboratories worldwide. An increasingly wide range of prokaryotic and eukaryotic species are being studied with regard to their sensing of diverse chemical and physical stimuli, including nutrients, toxins, intercellular signaling molecules, redox indicators, light, pressure, magnetic fields, and surface contact, leading to adaptive responses affecting motile behavior, gene expression and/or development. The ease of manipulation of microorganisms has facilitated application of a broad range of techniques that have provided comprehensive descriptions of cellular behavior and its underlying molecular mechanisms. Systems and their molecular components have been probed at levels ranging from the whole organism down to atomic resolution using behavioral analyses; electrophysiology; genetics; molecular biology; biochemical and biophysical characterization; structural biology; single molecule, fluorescence and cryo-electron microscopy; computational modeling; bioinformatics and genomic analyses. Several model systems such as bacterial chemotaxis and motility, fruiting body formation in Myxococcus xanthus, and motility and development in Dictyostelium discoideum have traditionally been a focus of this meeting. By providing a basis for assessment of similarities and differences in mechanisms, understanding of these pathways has advanced the study of many other microbial sensing systems. This conference aims to bring together researchers investigating different prokaryotic and eukaryotic microbial systems using diverse approaches to compare data, share methodologies and ideas, and seek to understand the fundamental principles underlying sensory responses. Topic areas include: (1) Receptor Sensing and Signaling; (2) Intracellular Signaling (two-component, c-di-GMP, c-AMP, etc.); (3) Intracellular Localization and the Cytoskeleton; (4) Motors and Motility; (5) Differentiation and Development; (6) Host/Pathogen and Host/Symbiont Interactions; (7) Intercellular Communication; (8) Microbes and the Environment; and (9) Modeling Signaling Pathways.

  9. Quantum superposition, entanglement, and state teleportation of a microorganism on an electromechanical oscillator

    E-Print Network [OSTI]

    Tongcang Li; Zhang-Qi Yin

    2015-09-12

    Schr\\"odinger's thought experiment to prepare a cat in a superposition of both alive and dead states reveals profound consequences of quantum mechanics and has attracted enormous interests. Here we propose a straightforward method to create quantum superposition states of a living microorganism by putting a small bacterium on top of an electromechanical oscillator. Our proposal is based on recent developments that the center-of-mass oscillation of a 15-$\\mu$m-diameter aluminium membrane has been cooled to its quantum ground state [Nature 475, 359 (2011)], and entangled with a microwave field [Science, 342, 710 (2013)]. A microorganism with a mass much smaller than the mass of the electromechanical membrane will not significantly affect the quality factor of the membrane and can be cooled to the quantum ground state together with the membrane. Quantum superposition and teleportation of its center-of-mass motion state can be realized with the help of superconducting microwave circuits. More importantly, the internal states of a microorganism, such as the electron spin of a glycine radical, can be prepared in a quantum superposition state and entangled with its center-of-mass motion. Our proposal can be realized with state-of-art technologies. The proposed setup is also a quantum-limited magnetic resonance force microscope (MRFM) that not only can detect the existence of an electron spin, but also can coherently manipulate and detect the quantum state of the spin.

  10. Non-indigenous microorganisms in the Antarctic: assessing the risks

    E-Print Network [OSTI]

    Vincent, Warwick F.

    Non-indigenous microorganisms in the Antarctic: assessing the risks Don A. Cowan1 , Steven L. Chown wastes at these sites, little has been done to prevent the introduction of non-indigenous microorganisms largely restricted to the impacts of human and animal pathogens (including viruses) [7,8,13] and organisms

  11. Studies on the activities of rumen microorganisms in vitro 

    E-Print Network [OSTI]

    Johnson, Carl Edward

    1957-01-01

    utilizaticn and volatile fatty acid production. Uolstile iatty acids are generally considered to be by-products of rumirM ferment"tion which the ruminant utilizes for enerI0', inter- mediates for ti sue biosynthesis, and milk production. However, Bentley...

  12. Deciphering Active Estrogen-Degrading Microorganisms in Bioreactors 

    E-Print Network [OSTI]

    Roh, Hyung Keun

    2010-10-12

    degraders (strains KC8 and ammonia-oxidizing bacteria (AOB)) and amoA gene (associated with ammonia oxidation) to total bacteria decreased as SRT increased in SBRs. These observations correspond to the decreasing percentages of 17 beta-estradiol biodegraded...

  13. METHANE INCORPORATION BY PROCARYOTIC PHOTOSYNTHETIC MICROORGANISMS

    E-Print Network [OSTI]

    Norton, Charles J.; Kirk, Martha; Calvin, Melvin

    1970-01-01

    11. As predicted from Dalton's law, C 14 activities inIN AQ. PHASE FROM DALTON'S LAW,? C ACTIVITY IN LIQUIDconstancy predicted by Dalton's law ll : Pa~ Solubility of

  14. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, James L. (Fayetteville, AR); Clausen, Edgar C. (Fayetteville, AR)

    1992-01-01

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H.sub.2 O and/or CO.sub.2 and H.sub.2 in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate.

  15. Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism

    DOE Patents [OSTI]

    Gaddy, J.L.; Clausen, E.C.

    1992-12-22

    A newly discovered microorganism was isolated in a biologically pure culture and designated Clostridium ljungdahlii, having the identifying characteristics of ATCC No. 49587. Cultured in an aqueous nutrient medium under anaerobic conditions, this microorganism is capable of producing ethanol and acetate from CO and H[sub 2]O and/or CO[sub 2] and H[sub 2] in synthesis gas. Under optimal growth conditions, the microorganism produces acetate in preference to ethanol. Conversely, under non-growth conditions, ethanol production is favored over acetate. 3 figs.

  16. Selective microorganism concentration using a dielectrophoresis-based microfabricated device

    E-Print Network [OSTI]

    Pucha?a, Katarzyna Anna

    2007-01-01

    Detection of pathogenic microorganisms is a significant challenge in medicine, environmental protection and biological threat safety because samples are often contaminated. This work presents a method of separating bacterial ...

  17. RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms,

    E-Print Network [OSTI]

    Goldstein, Raymond E.

    RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms, from individuals to concentrated non-pathogenic soil bacteria are rod-shaped (Fig. 1). Their length ranges from 2 to 8 lm, depending

  18. RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms,

    E-Print Network [OSTI]

    Cortez, Ricardo

    RESEARCH ARTICLE Fluid dynamics of self-propelled microorganisms, from individuals to concentrated00348-007-0387-y #12;Individual cells of these generally non-pathogenic soil bacteria are rod

  19. Electrical characterization of micro-organisms using microfabricated devices

    E-Print Network [OSTI]

    Bashir, Rashid

    . Kosari and G. Vasmatzis Mayo Clinic, Rochester, Minnesota 55905 A. Bhunia Department of Food Sciences by the micro-organisms when traversing through the pore. © 2002 American Vacuum Society. DOI: 10

  20. Microorganisms having enhanced tolerance to inhibitors and stress

    DOE Patents [OSTI]

    Brown, Steven D.; Yang, Shihui

    2014-07-29

    The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

  1. Microorganisms having enhanced resistance to acetate and methods of use

    DOE Patents [OSTI]

    Brown, Steven D; Yang, Shihui

    2014-10-21

    The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.

  2. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    solve the energy crisis through biological methods, including genetically engineering algae and cyanobacteria. Create a Sustainable Future: Living Living a Sustainable Future How...

  3. Nonequilibrium dissipation in living oocytes

    E-Print Network [OSTI]

    Étienne Fodor; Wylie W. Ahmed; Maria Almonacid; Matthias Bussonnier; Nir S. Gov; Marie-Hélène Verlhac; Timo Betz; Paolo Visco; Frédéric van Wijland

    2015-11-03

    Living organisms are inherently out-of-equilibrium systems. We employ new developments in stochastic energetics and rely on a minimal microscopic model to predict the amount of mechanical energy dissipated by such dynamics. Our model includes complex rheological effects and nonequilibrium stochastic forces. By performing active microrheology and tracking micron-sized vesicles in the cytoplasm of living oocytes, we provide unprecedented measurements of the spectrum of dissipated energy. We show that our model is fully consistent with the experimental data, and we use it to offer predictions for the injection and dissipation energy scales involved in active fluctuations.

  4. Physical and Chemical Factors Influencing the Transport and Fate of Microorganisms in Soils With Preferential Flow

    E-Print Network [OSTI]

    Wang, Yusong

    2013-01-01

    water resources from pathogenic microorganisms and contaminants associated with the colloidal phase, and to optimize bioremediation

  5. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control

    SciTech Connect (OSTI)

    Sayler, G.S.; Cox, C.D.; Ripp, S.; Nivens, D.E.; Werner, C.; Ahn, Y.; Matrubutham, U.; Burlage, R.

    1998-11-01

    On October 30, 1996, the US Environmental Protection Agency (EPA) commenced the first test release of genetically engineered microorganisms (GEMs) for use in bioremediation. The specific objectives of the investigation were multifaceted and include (1) testing the hypothesis that a GEM can be successfully introduced and maintained in a bioremediation process, (2) testing the concept of using, at the field scale, reporter organisms for direct bioremediation process monitoring and control, and (3) acquiring data that can be used in risk assessment decision making and protocol development for future field release applications of GEMs. The genetically engineered strain under investigation is Pseudomonas fluorescens strain HK44 (King et al., 1990). The original P. fluorescens parent strain was isolated from polycyclic aromatic hydrocarbon (PAH) contaminated manufactured gas plant soil. Thus, this bacterium is able to biodegrade naphthalene (as well as other substituted naphthalenes and other PAHs) and is able to function as a living bioluminescent reporter for the presence of naphthalene contamination, its bioavailability, and the functional process of biodegradation. A unique component of this field investigation was the availability of an array of large subsurface soil lysimeters. This article describes the experience associated with the release of a genetically modified microorganism, the lysimeter facility and its associated instrumentation, as well as representative data collected during the first eighteen months of operation.

  6. Discordance between living and death assemblages as evidence for anthropogenic ecological change

    E-Print Network [OSTI]

    . ecological baseline eutrophication marine communities paleoecology Human activities affect living systems

  7. Liquid Fuel from Heat-Loving Microorganisms: H2-Dependent Conversion of CO2 to Liquid Electrofuels by Extremely Thermophilic Archaea

    SciTech Connect (OSTI)

    None

    2010-07-01

    Electrofuels Project: NC State is working with the University of Georgia to create Electrofuels from primitive organisms called extremophiles that evolved before photosynthetic organisms and live in extreme, hot water environments with temperatures ranging from 167-212 degrees Fahrenheit The team is genetically engineering these microorganisms so they can use hydrogen to turn carbon dioxide directly into alcohol-based fuels. High temperatures are required to distill the biofuels from the water where the organisms live, but the heat-tolerant organisms will continue to thrive even as the biofuels are being distilled—making the fuel-production process more efficient. The microorganisms don’t require light, so they can be grown anywhere—inside a dark reactor or even in an underground facility.

  8. In Situ Survival of Genetically Engineered Microorganisms in a Tropical

    E-Print Network [OSTI]

    Hazen, Terry

    . Alvarez, G. M. Yumet, and C. L. Santiago Department of Biology, P.O. Box 23360, University of Puerto Rico, Rio Piedras, Puerto Rico 00931-3360 T. C. Hazen Savannah River Laboratory, Environmental Sciences microorganisms (GEMs) and their interactions with the environmental microbiota of a tropical river

  9. Early Detection Saves Lives

    Office of Energy Efficiency and Renewable Energy (EERE)

    Former Department of Energy (DOE) workers tell how medical screening helped them lead healthier and longer lives.

  10. FUTURE LOGISTICS LIVING LABORATORY

    E-Print Network [OSTI]

    Heiser, Gernot

    FUTURE LOGISTICS LIVING LABORATORY Delivering Innovation The Future Logistics Living Lab that will provide logistics solutions for the future. The Living Lab is a demonstration, exhibition and work space by a group of logistics companies, research organisations, universities, and IT providers that includes NICTA

  11. Studies of unicellular micro-organisms Saccharomyces cerevisiae by means of Positron Annihilation Lifetime Spectroscopy

    E-Print Network [OSTI]

    Kubicz, E; Zgardzi?ska, B; Bednarski, T; Bia?as, P; Czerwi?ski, E; Gajos, A; Gorgol, M; Kami?ska, D; Kap?on, ?; Kochanowski, A; Korcyl, G; Kowalski, P; Kozik, T; Krzemie?, W; Nied?wiecki, S; Pa?ka, M; Raczy?ski, L; Rajfur, Z; Rudy, Z; Rundel, O; Sharma, N G; Silarski, M; S?omski, A; Strzelecki, A; Wieczorek, A; Wi?licki, W; Zieli?ski, M; Moskal, P

    2015-01-01

    Results of Positron Annihilation Lifetime Spectroscopy (PALS) and microscopic studies on simple microorganisms: brewing yeasts are presented. Lifetime of ortho - positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer lived component) for lyophilised and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water - the main component of the cell - affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilised, dried and wet yeasts with best possible resolution were obtained using Inverted Microscopy (IM) and Environmental Scanning Electron Microscopy (ESEM) methods. As a result visible changes to the surface of the cell membrane were observed in ESEM images.

  12. Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic Environment: A Critical Review

    E-Print Network [OSTI]

    Mechanisms Regulating Mercury Bioavailability for Methylating Microorganisms in the Aquatic by anaerobic bacteria. In this Review, we evaluate the current state of knowledge regarding the mechanisms the uptake of inorganic mercury to these microorganisms. Our understanding of the mechanisms behind

  13. Aerobic microorganism for the degradation of chlorinated aliphatic hydrocarbons

    DOE Patents [OSTI]

    Fliermans, Carl B. (Augusta, GA)

    1989-01-01

    A chlorinated aliphatic hydrocarbon-degrading microorganism, having American Type Culture Collection accession numbers ATCC 53570 and 53571, in a biologically pure culture aseptically collected from a deep subsurface habitat and enhanced, mineralizes trichloroethylene and tetrachloroethylene to HCl, H.sub.2 O and Co.sub.2 under aerobic conditions stimulated by methane, acetate, methanol, tryptone-yeast extract, propane and propane-methane.

  14. Use of Stable Isotopes in Forensic Analysis of Microorganisms

    SciTech Connect (OSTI)

    Kreuzer-Martin, Helen W.; Hegg, Eric L.

    2012-01-18

    The use of isotopic signatures for forensic analysis of biological materials is well-established, and the same general principles that apply to interpretation of stable isotope content of C, N, O, and H apply to the analysis of microorganisms. Heterotrophic microorganisms derive their isotopic content from their growth substrates, which are largely plant and animal products, and the water in their culture medium. Thus the isotope signatures of microbes are tied to their growth environment. The C, N, O, and H isotope ratios of spores have been demonstrated to constitute highly discriminating signatures for sample matching. They can rule out specific samples of media and/or water as possible production media, and can predict isotope ratio ranges of the culture media and water used to produce a given sample. These applications have been developed and tested through analyses of approximately 250 samples of Bacillus subtilis spores and over 500 samples of culture media, providing a strong statistical basis for data interpretation. A Bayesian statistical framework for integrating stable isotope data with other types of signatures derived from microorganisms has been able to characterize the culture medium used to produce spores of various Bacillus species, leveraging isotopic differences in different medium types and demonstrating the power of data integration for forensic investigations.

  15. Encapsulation method for maintaining biodecontamination activity

    DOE Patents [OSTI]

    Rogers, Robert D. (Idaho Falls, ID); Hamilton, Melinda A. (Idaho Falls, ID); Nelson, Lee O. (Idaho Falls, ID); Benson, Jennifer (Cockermouth, GB); Green, Martin J. (Wooton, GB); Milner, Timothy N. (Centerville, VA)

    2002-01-01

    A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.

  16. Encapsulation method for maintaining biodecontamination activity

    DOE Patents [OSTI]

    Rogers, Robert D.; Hamilton, Melinda A.; Nelson, Lee O.; Benson, Jennifer; Green, Martin J.; Milner, Timothy N.

    2006-04-11

    A method for maintaining the viability and subsequent activity of microorganisms utilized in a variety of environments to promote biodecontamination of surfaces. One application involves the decontamination of concrete surfaces. Encapsulation of microbial influenced degradation (MID) microorganisms has shown that MID activity is effectively maintained under passive conditions, that is, without manual addition of moisture or nutrients, for an extended period of time.

  17. ACCOUNTING ROADMAP TRANSFORMING LIVES

    E-Print Network [OSTI]

    ACCOUNTING ROADMAP TO SUCCESS THE TRANSFORMING LIVES COLLEGE OF BUSINESS Department of Accounting #12;TABLEOFCONTENTS Greetings from Accounting Department Chair ..............................2 What is Accounting? .......................................................................4 Successful Study

  18. Microorganisms to Speed Production of Biofuels - Energy Innovation Portal

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration wouldMass map shines light on darkMicroorganisms to Speed Production of Biofuels Oak Ridge

  19. Effects of remediation amendments on vadose zone microorganisms

    SciTech Connect (OSTI)

    Miller, Hannah M.; Tilton, Fred A.

    2012-08-10

    Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring microbial metabolic responses to remediation amendments in batch and column studies using Deep Vadose Zone Sediments. Initial studies of the microbes from Hanford 200 area deep vadose zone sediment showed surfactants sodium dodecyl sulfate (SDS) and cocamidopropyl betaine (CAPB) and remediation amendment calcium polysulfide (CPS) had no affect on microbial growth using BiologTM Ecoplates. To move towards a more realistic field analog, soil columns were packed with Hanford 200 Area sediment. Once microbial growth in the column was verified by observing growth of the effluent solution on tryptic soy agar plates, remedial surfactants were injected into the columns, and the resulting metabolic diversity was measured. Results suggest surfactant sodium dodecyl sulfate (SDS) stimulates microbial growth. The soil columns were also visualized using X-ray microtomography to inspect soil packing and possibly probe for evidence of biofilms. Overall, BiologTM Ecoplates provide a rapid assay to predict effects of remediation amendments on Hanford 200 area deep vadose zone microorganisms.

  20. FROM MATHEMATICS FOR LIVING TO LIVING FOR MATHEMATICS

    E-Print Network [OSTI]

    Spagnolo, Filippo

    FROM MATHEMATICS FOR LIVING TO LIVING FOR MATHEMATICS George Malaty, University of Joensuu, Finland "Life is good for only two things, discovering mathematics and teaching mathematics". Siméon Poisson (1781-1840) Mathematics for living and living for mathematics are related to the goals of mathematics

  1. ACCOUNTING ROADMAP TRANSFORMING LIVES

    E-Print Network [OSTI]

    Collett Jr., Jeffrey L.

    ACCOUNTING ROADMAP TO SUCCESS THE TRANSFORMING LIVES COLLEGE OF BUSINESS Department of Accounting #12;TABLEOFCONTENTS Greetings from Accounting Department Chair 2 What is Accounting? 4 Successful Accounting Career Paths 8 Careers in Managerial Accounting 9 Careers in Government/Not-for-Profit (GNP

  2. Live Paint: Painting with Procedural Multiscale Textures

    E-Print Network [OSTI]

    Live Paint: Painting with Procedural Multiscale Textures Ken Perlin Media Research Laboratory´atica Pura e Aplicada Abstract We present actively procedural multiresolution paint textures. Tex- ture drawing tool that can be used in a multires- olution paint system. They provide a mechanism to generate

  3. Live Paint: Painting with Procedural Multiscale Textures

    E-Print Network [OSTI]

    de Figueiredo, Luiz Henrique

    Live Paint: Painting with Procedural Multiscale Textures (Expanded Version) Ken Perlin Media -- Instituto de Matemâ??atica Pura e Aplicada Abstract We present actively procedural multiresolution paint constitute a powerful drawing tool that can be used in a multires­ olution paint system. They provide

  4. Assessing the transport and fate of bioengineered microorganisms in the environment

    SciTech Connect (OSTI)

    Barnthouse, L.W.; Palumbo, A.V.

    1985-01-01

    We review the methods currently available for quantifying the transport and fate of microbes in atmospheric and aqueous media and assess their adequacy for purposes of risk assessment. We review the literature on transport and fate of microorganisms, including studies of: (1) pathways of migration, (2) the survival of microorganisms during transport and fate. In addition, we review the transport and fate models that have been used in environmental risk assessments for radionuclides and toxic chemicals and evaluate their applicability to the problem of assessing environmental risks of bioengineered microorganisms.

  5. Living olefin polymerization processes

    DOE Patents [OSTI]

    Schrock, R.R.; Baumann, R.

    1999-03-30

    Processes for the living polymerization of olefin monomers with terminal carbon-carbon double bonds are disclosed. The processes employ initiators that include a metal atom and a ligand having two group 15 atoms and a group 16 atom or three group 15 atoms. The ligand is bonded to the metal atom through two anionic or covalent bonds and a dative bond. The initiators are particularly stable under reaction conditions in the absence of olefin monomer. The processes provide polymers having low polydispersities, especially block copolymers having low polydispersities. It is an additional advantage of these processes that, during block copolymer synthesis, a relatively small amount of homopolymer is formed.

  6. Living a Sustainable Future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenterLeeincreases |Living a

  7. Nucleic acid molecules conferring enhanced ethanol tolerance and microorganisms having enhanced tolerance to ethanol

    DOE Patents [OSTI]

    Brown, Steven; Guss, Adam; Yang, Shihui; Karpinets, Tatiana; Lynd, Lee; Shao, Xiongjun

    2014-01-14

    The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.

  8. Representation of Dormant and Active Microbial Dynamics for Ecosystem Modeling

    SciTech Connect (OSTI)

    Wang, Gangsheng; Mayes, Melanie; Gu, Lianhong; Schadt, Christopher Warren

    2014-01-01

    Dormancy is an essential strategy for microorganisms to cope with environmental stress. However, global ecosystem models typically ignore microbial dormancy, resulting in notable model uncertainties. To facilitate the consideration of dormancy in these large-scale models, we propose a new microbial physiology component that works for a wide range of substrate availabilities. This new model is based on microbial physiological states and the major parameters are the maximum specific growth and maintenance rates of active microbes and the ratio of dormant to active maintenance rates. A major improvement of our model over extant models is that it can explain the low active microbial fractions commonly observed in undisturbed soils. Our new model shows that the exponentially-increasing respiration from substrate-induced respiration experiments can only be used to determine the maximum specific growth rate and initial active microbial biomass, while the respiration data representing both exponentially-increasing and non-exponentially-increasing phases can robustly determine a range of key parameters including the initial total live biomass, initial active fraction, the maximum specific growth and maintenance rates, and the half-saturation constant. Our new model can be incorporated into existing ecosystem models to account for dormancy in microbially-driven processes and to provide improved estimates of microbial activities.

  9. Final Technical Report: Viral Infection of Subsurface Microorganisms and Metal/Radionuclide Transport

    SciTech Connect (OSTI)

    Weber, Karrie A.; Bender, Kelly S.; Li, Yusong

    2013-09-28

    Microbially mediated metabolisms have been identified as a significant factor either directly or indirectly impacting the fate and transport of heavy metal/radionuclide contaminants. To date microorganisms have been isolated from contaminated environments. Examination of annotated finished genome sequences of many of these subsurface isolates from DOE sites, revealed evidence of prior viral infection. To date the role that viruses play influencing microbial mortality and the resulting community structure which directly influences biogeochemical cycling in soils and sedimentary environments remains poorly understood. The objective of this exploratory study was to investigate the role of viral infection of subsurface bacteria and the formation of contaminant-bearing viral particles. This objective was approached by examining the following working hypotheses: (i) subsurface microorganisms are susceptible to viral infections by the indigenous subsurface viral community, and (ii) viral surfaces will adsorb heavy metals and radionuclides. Our results have addressed basic research needed to accomplish the BER Long Term Measure to provide sufficient scientific understanding such that DOE sites would be able to incorporate coupled physical, chemical and biological processes into decision making for environmental remediation or natural attenuation and long-term stewardship by establishing viral-microbial relationships on the subsequent fate and transport of heavy metals and radionuclides. Here we demonstrated that viruses play a significant role in microbial mortality and community structure in terrestrial subsurface sedimentary systems. The production of viral-like particles within subsurface sediments in response to biostimulation with dissolved organic carbon and a terminal electron acceptor resulted in the production of viral-like particles. Organic carbon alone did not result in significant viral production and required the addition of a terminal electron acceptor (nitrate), indicating that nutrients are not limiting viral production, but rather substrates that can be converted into energy for host metabolism. Our results also revealed that cell abundance was not correlated to the mineralization of organic carbon, but rather viruses were positively correlated with carbon mineralization. This is a result of viral-mediated cell lysis and demonstrates that viruses are sensitive indicators of microbial activity. Viruses as an indicator of microbial activity was not unique to batch culture studies as results obtained from an in situ field experiment conducted at the DOE Old Rifle Field site. This study revealed that viral abundance increased in response to the injection of oxygenated groundwater and influx of dissolved organic carbon whereas cell abundance changes were minimal. However, the extent to which viral-mediated cell lysis alters organic matter pools subsequently influencing microbial community structure and biogeochemical function remains a critical question in subsurface biogeochemical cycling. The production of significant numbers of viruses in groundwater has implications for nanoparticulate metal as well as carbon transport in groundwater. We have demonstrated that the virus surface is reactive and will adsorb heavy metals. Thus viruses can promote colloidal contaminant mobility. Interestingly, the presence of heavy metals has a positive effect on infectivity of the phage, increasing phage infection which could lead to further production of viruses. Together, the results indicate that the sorption of metals to the surface of viruses could not only contribute to nanoparticulate metal as well as carbon transport but could also enhance infectivity further contributing to cell lysis which could subsequently influence biogeochemical cycling. As more viruses infect host microbial populations the high concentration of metals would enhance infection, resulting in cell lysis, and decreasing the metabolically active host population while yielding greater numbers of viruses capable of transporting contaminats. Additional studie

  10. Bacteria Marinobacter aquaeolei. Community Living. Microbes living in coral provide

    E-Print Network [OSTI]

    Bacteria Marinobacter aquaeolei. Community Living. Microbes living in coral provide nutrients Cleaning up. Bacteria are being tested for use as cleaning agents of toxic chemicals and pollutants in our.The big red and green dots (numbering about 1,000) are bacteria, and the very small background dots (about

  11. The Social Lives of Microbes

    E-Print Network [OSTI]

    West, Stuart

    The Social Lives of Microbes Stuart A. West,1 Stephen P. Diggle,2 Angus Buckling,3 Andy Gardner,1 evolution Abstract Our understanding of the social lives of microbes has been revolu- tionized over the past that microbes indulge in a variety of social behaviors involving com- plex systems of cooperation, communication

  12. LIVING SOIL Master Gardener College

    E-Print Network [OSTI]

    Isaacs, Rufus

    LIVING SOIL Master Gardener College George W. Bird, Professor, MSU (June 9, 2012) #12;#12;Living Soil References G. W. Bird, Professor Michigan State University birdg@msu.edu http://www.ent.msu.edu/Directory/Facultypages/bird/tabid/133/Default.aspx · Brady, N. and R. Weil. 2002. Nature and Properties of Soils (13th ed) Prentice Hall

  13. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    SciTech Connect (OSTI)

    Gu, April Z; Wan, Kai-tak

    2014-09-02

    This project aims to explore and develop enabling methodology and techniques for nano-scale characterization of microbe cell surface contact mechanics, interactions and adhesion quantities that allow for identification and quantification of indicative properties related to microorganism migration and transport behavior in porous media and in subsurface environments. Microbe transport has wide impact and therefore is of great interest in various environmental applications such as in situ or enhanced subsurface bioremediation,filtration processes for water and wastewater treatments and protection of drinking water supplies. Although great progress has been made towards understanding the identities and activities of these microorganisms in the subsurface, to date, little is known of the mechanisms that govern the mobility and transport of microorganisms in DOE’s contaminated sites, making the outcomes of in situ natural attenuation or contaminant stability enhancement unpredictable. Conventionally, movement of microorganisms was believed to follows the rules governing solute (particle) transport. However, recent studies revealed that cell surface properties, especially those pertaining to cell attachment/adhesion and aggregation behavior, can cause the microbe behavior to deviate from non-viable particles and hence greatly influence the mobility and distribution of microorganisms in porous media.This complexity highlights the need to obtain detailed information of cell-cell and cell-surface interactions in order to improve and refine the conceptual and quantitative model development for fate and transport of microorganisms and contaminant in subsurface. Traditional cell surface characterization methods are not sufficient to fully predict the deposition rates and transport behaviors of microorganism observed. A breakthrough of methodology that would allow for quantitative and molecular-level description of intrinsic cell surface properties indicative for cell-surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  14. Blood Banking in Living Droplets

    E-Print Network [OSTI]

    Samot, Josh

    Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional ...

  15. The Living Culture of Nigeria

    E-Print Network [OSTI]

    Biobaku, Saburi O.; Aniakor, Chike A.

    1977-01-01

    ImK ~IE.W THE uv:rn:; aJLWRE OF NIGERIA edited by Saburi 0 .Lagos: Thanas Nelson (Nigeria) Ltd. , Color Illustrations,86 The Living Cu'lture of Nigeria edited by Professor Saburi

  16. To establish infection, pathogenic microorganisms have evolved many strategies to circumvent host defences and

    E-Print Network [OSTI]

    Nizet, Victor

    To establish infection, pathogenic microorganisms have evolved many strategies to circumvent host trafficking pathways to and from the host cell surface, which promotes pathogen entry, replication or escape of proteins such as Toll-like receptor 4 (TLR4) to phagosomes for receptor-mediated phagocytosis of pathogens8

  17. RESPONSES OF BENTHIC MICROORGANISMS (THECAMOEBIANS) TO OIL SANDS PROCESS-AFFECTED MATERIALS; PROVIDING

    E-Print Network [OSTI]

    Patterson, Timothy

    RESPONSES OF BENTHIC MICROORGANISMS (THECAMOEBIANS) TO OIL SANDS PROCESS-AFFECTED MATERIALS an important role in reclamation options for fluid tailings (OSPW/M) at surface oil sands operations. Through of oil sands operation have been compared. An index of response to stress has been compiled with the goal

  18. What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under

    E-Print Network [OSTI]

    Mukhtar, Saqib

    What is compost? Composting refers to biological decomposition and stabilization of organic materials by microorganisms under aerobic conditions (in the presence of oxygen). During the composting is production of good-quality compost that is biologically stable, relatively uniform in appearance, free

  19. INTRODUCTION The contribution of micro-organisms to amorphous silica precipitation in modern

    E-Print Network [OSTI]

    Benning, Liane G.

    INTRODUCTION The contribution of micro-organisms to amorphous silica precipitation in modern, University of Guelph, Canada 3 Institute of Geological and Nuclear Sciences, Wairakei Research Centre, Taupo as geothermal energy sources and as a proxy to understanding the formation of epithermal ore deposits, which

  20. The impact of soil microorganisms on the global budget of 18

    E-Print Network [OSTI]

    sizes for CA- containing soil microorganisms. Including accelerated soil hydra- tion in global model Biotechnologie, Service de Biologie Ve´ge´tale et de Microbiologie Environnementale, Commissariat a` l'EnergieDepartment of Environmental Sciences and Energy Research, Weizmann Institute of Science, Rehovot, 76100, Israel; h

  1. Meso-scale turbulence in living fluids

    E-Print Network [OSTI]

    Wensink, Henricus H; Heidenreich, Sebastian; Drescher, Knut; Goldstein, Raymond E; Löwen, Hartmut; Yeomans, Julia M

    2012-01-01

    Turbulence is ubiquitous, from oceanic currents to small-scale biological and quantum systems. Self-sustained turbulent motion in microbial suspensions presents an intriguing example of collective dynamical behavior amongst the simplest forms of life, and is important for fluid mixing and molecular transport on the microscale. The mathematical characterization of turbulence phenomena in active non-equilibrium fluids proves even more difficult than for conventional liquids or gases. It is not known which features of turbulent phases in living matter are universal or system-specific, or which generalizations of the Navier-Stokes equations are able to describe them adequately. Here, we combine experiments, particle simulations, and continuum theory to identify the statistical properties of self-sustained meso-scale turbulence in active systems. To study how dimensionality and boundary conditions affect collective bacterial dynamics, we measured energy spectra and structure functions in dense Bacillus subtilis su...

  2. South Atlantic OCS area living marine resources study. Volume I: an investigation of live bottom habitats south of Cape Fear, North Carolina. Final report

    SciTech Connect (OSTI)

    Not Available

    1981-10-01

    The major objectives of this study were to (1) characterize benthic and nektonic communities associated with representative live bottom habitats on the continental shelf of the South Atlantic Bight, and (2) evaluate factors which might influence these communities, particularly the potential for impact by offshore oil and gas activities. The study areas include nine live bottom areas located off South Carolina, Georgia and Florida.

  3. Natural Oil Production from Microorganisms: Bioprocess and Microbe Engineering for Total Carbon Utilization in Biofuel Production

    SciTech Connect (OSTI)

    2010-07-15

    Electrofuels Project: MIT is using carbon dioxide (CO2) and hydrogen generated from electricity to produce natural oils that can be upgraded to hydrocarbon fuels. MIT has designed a 2-stage biofuel production system. In the first stage, hydrogen and CO2 are fed to a microorganism capable of converting these feedstocks to a 2-carbon compound called acetate. In the second stage, acetate is delivered to a different microorganism that can use the acetate to grow and produce oil. The oil can be removed from the reactor tank and chemically converted to various hydrocarbons. The electricity for the process could be supplied from novel means currently in development, or more proven methods such as the combustion of municipal waste, which would also generate the required CO2 and enhance the overall efficiency of MIT’s biofuel-production system.

  4. Saving Lives and Mitigating Losses

    E-Print Network [OSTI]

    Duchowski, Andrew T.

    Saving Lives and Mitigating Losses Wind and Structural Engineering Research Facility #12;Clemson University's Wind and Structural Engineering Research (WiSER) Facility is a premier laboratory for the study of wind effects on structures. Testing to assess the structural performance of buildings and bridges can

  5. Electrosynthesis of Organic Compounds from Carbon Dioxide Is Catalyzed by a Diversity of Acetogenic Microorganisms

    SciTech Connect (OSTI)

    Nevin, KP; Hensley, SA; Franks, AE; Summers, ZM; Ou, JH; Woodard, TL; Snoeyenbos-West, OL; Lovley, DR

    2011-04-20

    Microbial electrosynthesis, a process in which microorganisms use electrons derived from electrodes to reduce carbon dioxide to multicarbon, extracellular organic compounds, is a potential strategy for capturing electrical energy in carbon-carbon bonds of readily stored and easily distributed products, such as transportation fuels. To date, only one organism, the acetogen Sporomusa ovata, has been shown to be capable of electrosynthesis. The purpose of this study was to determine if a wider range of microorganisms is capable of this process. Several other acetogenic bacteria, including two other Sporomusa species, Clostridium ljungdahlii, Clostridium aceticum, and Moorella thermoacetica, consumed current with the production of organic acids. In general acetate was the primary product, but 2-oxobutyrate and formate also were formed, with 2-oxobutyrate being the predominant identified product of electrosynthesis by C. aceticum. S. sphaeroides, C. ljungdahlii, and M. thermoacetica had high (> 80%) efficiencies of electrons consumed and recovered in identified products. The acetogen Acetobacterium woodii was unable to consume current. These results expand the known range of microorganisms capable of electrosynthesis, providing multiple options for the further optimization of this process.

  6. Use of the Crystal Diffraction Method to Study the Transmutation of Long-Lived Nuclides

    E-Print Network [OSTI]

    Titov, Anatoly

    1 Use of the Crystal Diffraction Method to Study the Transmutation of Long-Lived Nuclides V. L nuclides in nuclear waste products transmuta- tion. It is known that more than 90% of the waste activity after 10 years storage provides with two long- lived 90 Sr and 137 Cs nuclides. Their transmutation

  7. Engineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    James C. Liao

    2012-05-22

    This project is a collaboration with F. R. Tabita of Ohio State. Our major goal is to understand the factors and regulatory mechanisms that influence hydrogen production. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Our part of the project was to develop a modeling technique to investigate the metabolic network in connection to hydrogen production and regulation. Organisms must balance the pathways that generate and consume reducing power in order to maintain redox homeostasis to achieve growth. Maintaining this homeostasis in the nonsulfur purple photosynthetic bacteria is a complex feat with many avenues that can lead to balance, as these organisms possess versatile metabolic capabilities including anoxygenic photosynthesis, aerobic or anaerobic respiration, and fermentation. Growth is achieved by using H{sub 2} as an electron donor and CO{sub 2} as a carbon source during photoautotrophic and chemoautotrophic growth, where CO{sub 2} is fixed via the Calvin-Benson-Bassham (CBB) cycle. Photoheterotrophic growth can also occur when alternative organic carbon compounds are utilized as both the carbon source and electron donor. Regardless of the growth mode, excess reducing equivalents generated as a result of oxidative processes, must be transferred to terminal electron acceptors, thus insuring that redox homeostasis is maintained in the cell. Possible terminal acceptors include O{sub 2}, CO{sub 2}, organic carbon, or various oxyanions. Cells possess regulatory mechanisms to balance the activity of the pathways which supply energy, such as photosynthesis, and those that consume energy, such as CO{sub 2} assimilation or N{sub 2} fixation. The major route for CO{sub 2} assimilation is the CBB reductive pentose phosphate pathway, whose key enzyme is ribulose 1,5-biphosphate carboxylase/oxygenase (RubisCO). In addition to providing virtually all cellular carbon during autotrophic metabolism, RubisCO-mediated CO{sub 2} assimilation is also very important for nonsulfur purple photosynthetic bacteria under photoheterotrophic growth conditions since CO{sub 2} becomes the major electron sink under these conditions. In this work, Ensemble Modeling (EM) was developed to examine the behavior of CBB-compromised RubisCO knockout mutant strains of the nonsulfur purple photosynthetic bacterium Rhodobacter sphaeroides. Mathematical models of metabolism can be a great aid in studying the effects of large perturbations to the system, such as the inactivation of RubisCO. Due to the complex and highly-interconnected nature of these networks, it is not a trivial process to understand what the effect of perturbations to the metabolic network will be, or vice versa, what enzymatic perturbations are necessary to yield a desired effect. Flux distribution is controlled by multiple enzymes in the network, often indirectly linked to the pathways of interest. Further, depending on the state of the cell and the environmental conditions, the effect of a perturbation may center around how it effects the carbon flow in the network, the balancing of cofactors, or both. Thus, it is desirable to develop mathematical models to describe, understand, and predict network behavior. Through the development of such models, one may gain the ability to generate a set of testable hypotheses for system behavior.

  8. Meso-scale turbulence in living fluids

    E-Print Network [OSTI]

    Henricus H. Wensink; Jörn Dunkel; Sebastian Heidenreich; Knut Drescher; Raymond E. Goldstein; Hartmut Löwen; Julia M. Yeomans

    2012-08-21

    Turbulence is ubiquitous, from oceanic currents to small-scale biological and quantum systems. Self-sustained turbulent motion in microbial suspensions presents an intriguing example of collective dynamical behavior amongst the simplest forms of life, and is important for fluid mixing and molecular transport on the microscale. The mathematical characterization of turbulence phenomena in active non-equilibrium fluids proves even more difficult than for conventional liquids or gases. It is not known which features of turbulent phases in living matter are universal or system-specific, or which generalizations of the Navier-Stokes equations are able to describe them adequately. Here, we combine experiments, particle simulations, and continuum theory to identify the statistical properties of self-sustained meso-scale turbulence in active systems. To study how dimensionality and boundary conditions affect collective bacterial dynamics, we measured energy spectra and structure functions in dense Bacillus subtilis suspensions in quasi-2D and 3D geometries. Our experimental results for the bacterial flow statistics agree well with predictions from a minimal model for self-propelled rods, suggesting that at high concentrations the collective motion of the bacteria is dominated by short-range interactions. To provide a basis for future theoretical studies, we propose a minimal continuum model for incompressible bacterial flow. A detailed numerical analysis of the 2D case shows that this theory can reproduce many of the experimentally observed features of self-sustained active turbulence.

  9. Measuring Interference Between Live Datacenter Applications

    E-Print Network [OSTI]

    Cortes, Corinna

    Measuring Interference Between Live Datacenter Applications Melanie Kambadur Columbia University in datacenters due to contention over shared hardware resources. Unfortunately, understanding interference in live datacenters is more difficult than in controlled environments or on simpler architectures. Most

  10. Solo living across the adult lifecourse 

    E-Print Network [OSTI]

    Smith, Adam; Wasoff, Fran; Jamieson, Lynn

    This study has created snapshots of solo living in contemporary society and developed an understanding of the social and economic factors involved in transitions in and out of solo living.

  11. THE IMPACT OF MICROBIAL CHELATES ON MINERAL WEATHERING AND MICROBIAL METABOLIC ACTIVITY

    E-Print Network [OSTI]

    Kulczycki, Ezra

    2010-04-25

    Geological materials are composed of elements, which can be released into the environment by dissolution or desorption processes and directly impact the metabolic activity of microorganisms. If subjected to environmental ...

  12. Quantitative phase microscopy for the study of electromotility in living cells

    E-Print Network [OSTI]

    Oh, Seung-eun

    2010-01-01

    The electric activity of living cells is accompanied with changes in their optical and mechanical properties, which arise from the intrinsic biophysics of the cell membrane. These intrinsic changes can be used as an indicator ...

  13. Live Fire Range Environmental Assessment

    SciTech Connect (OSTI)

    1993-08-01

    The Central Training Academy (CTA) is a DOE Headquarters Organization located in Albuquerque, New Mexico, with the mission to effectively and efficiently educate and train personnel involved in the protection of vital national security interests of DOE. The CTA Live Fire Range (LFR), where most of the firearms and tactical training occurs, is a complex separate from the main campus. The purpose of the proposed action is to expand the LFR to allow more options of implementing required training. The Department of Energy has prepared this Environmental Assessment (EA) for the proposed construction and operation of an expanded Live Fire Range Facility at the Central Training Academy in Albuquerque, New Mexico. Based on the analysis in the EA, DOE has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required and DOE is issuing this Finding of No Significant Impact (FONSI).

  14. Detection and quantification of waterborne microorganisms using an image cytometer based on angular spatial frequency processing

    E-Print Network [OSTI]

    Pérez, Juan Miguel; Martínez, Pedro; Pruneri, Valerio

    2015-01-01

    We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off--the--shelf components, such as a light emitting diode (LED) source, a complementary metal--oxide--semiconductor (CMOS) image sensor, and a specific combination of optical lenses that allow, through an appropriate software, Fourier transform processing of the sample volume. Waterborne microorganisms, such as Escherichia coli (E. coli), Legionella pneumophila (L. pneumophila) and Phytoplankton, are detected by interrogating the volume sample either in a fluorescent or label-free mode, i.e. with or without fluorescein isothiocyanate (FITC) molecules attached to the micro-organisms, respectively. We achieve a sensitivity of 50 CFU/ml, which can be further increased to 0.2 CFU/ml by pre-concentrating an initial sample volume of 500 ml with an ad hoc fluidic system. We also prove the capability of the proposed image cytometer of diffe...

  15. Quantum Process in Living Cells

    E-Print Network [OSTI]

    Robert W. Finkel

    2012-10-04

    Quantum processes have been confirmed for various biological phenomena. Here we model a quantum process in cells based on coherent waves of established ultrafast energy transfers in water. We compute wave speed, ~156 km/s, and wavelength, ~9.3 nm, and determine that the waves retain local coherence. The model is compared with observations and diverse numerical applications lend support to the hypothesis that rapid energy transfers in water are characteristic of living cells. Close agreements are found for the dipole moment of water dimers, microwave radiation on yeast, and the Kleiber law of metabolic rates. We find a sphere with diameter ~20 nm is a lower bound for life in this theory. The quantum properties of the model suggest that cellular chemistry favors reactions that support perpetuation of the energy waves

  16. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, E.T.; Lin, M.

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70 C to 90 C, at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%. 68 figs.

  17. Process for producing modified microorganisms for oil treatment at high temperatures, pressures and salinity

    DOE Patents [OSTI]

    Premuzic, Eugene T. (East Moriches, NY); Lin, Mow (Rocky Point, NY)

    1996-02-20

    This invention relates to the preparation of new, modified organisms, through challenge growth processes, that are viable in the extreme temperature, pressure and pH conditions and salt concentrations of an oil reservoir and that are suitable for use in microbial enhanced oil recovery. The modified microorganisms of the present invention are used to enhance oil recovery and remove sulfur compounds and metals from the crude oil. The processes are comprised of steps which successively limit the carbon sources and increase the temperature, pressure and salinity of the media. This is done until microbial strains are obtained that are capable of growing in essentially crude oil as a carbon source and at a temperature range from about 70.degree. C. to 90.degree. C., at a pressure range from about 2,000 to 2,500 psi and at a salinity range from about 1.3 to 35%.

  18. Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

    SciTech Connect (OSTI)

    Kuk Lee, Sung; Chou, Howard; Ham, Timothy S.; Soon Lee, Taek; Keasling, Jay D.

    2009-12-02

    The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

  19. How sulphate-reducing microorganisms cope with stress: Lessons from systems biology

    SciTech Connect (OSTI)

    Zhou, J.; He, Q.; Hemme, C.L.; Mukhopadhyay, A.; Hillesland, K.; Zhou, A.; He, Z.; Nostrand, J.D. Van; Hazen, T.C.; Stahl, D.A.; Wall, J.D.; Arkin, A.P.

    2011-04-01

    Sulphate-reducing microorganisms (SRMs) are a phylogenetically diverse group of anaerobes encompassing distinct physiologies with a broad ecological distribution. As SRMs have important roles in the biogeochemical cycling of carbon, nitrogen, sulphur and various metals, an understanding of how these organisms respond to environmental stresses is of fundamental and practical importance. In this Review, we highlight recent applications of systems biology tools in studying the stress responses of SRMs, particularly Desulfovibrio spp., at the cell, population, community and ecosystem levels. The syntrophic lifestyle of SRMs is also discussed, with a focus on system-level analyses of adaptive mechanisms. Such information is important for understanding the microbiology of the global sulphur cycle and for developing biotechnological applications of SRMs for environmental remediation, energy production, biocorrosion control, wastewater treatment and mineral recovery.

  20. Lancaster Live/Work Townhome Prototype

    SciTech Connect (OSTI)

    2010-04-01

    This case study describes development of a prototype live-work townhome that is highly efficient at 45% energy savings (95% counting photovoltaic system).

  1. Nuclear Physics Technology Saves Lives | Jefferson Lab

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Nuclear Physics Technology Saves Lives January 11, 2006 Listen to this story Ribbon With early detection, breast cancer can often be treated successfully. There are over two...

  2. Extending the Operating Lives of Materials

    ScienceCinema (OSTI)

    Paul Jablonski

    2010-09-01

    Metallurgist Paul Jablonski discusses his role in developing processes that extend the operating temperatures and operating lives of materials used in energy applications.

  3. Connecticut Weatherization Project Improves Lives, Receives National...

    Broader source: Energy.gov (indexed) [DOE]

    Several energy-efficient improvements made to a senior care center in New Milford, Connecticut, are helping residents live healthier and more comfortable lifestyles. The upgrade...

  4. Marshall Islands: a study of diet and living patterns

    SciTech Connect (OSTI)

    Naidu, J.R.; Greenhouse, N.A.; Knight, G.; Craighead, E.C.

    1980-07-01

    This study summarizes information on diet and living patterns for the Marshallese. The data was derived from literature, answers to questionnaires, personal observations while living with the Marshallese for periods extending from months to years, and from direct participation in their activities. The results reflect the complex interactions of many influences, such as, the gathering of local foods the receipt of food aid through programs, such as, school-lunch, typhoon-relief, food distributed to populations displaced as a result of nuclear testing, and in recent times the availability of cash for the purchase of imported foods. The results identify these influences and are therefore restricted to local food diets while recognizing that the living patterns are changing as local food gathering is replaced by other food supplies. The data will therefore provide the necessary information for input into models that will assess the radiological impacts attributable to the inhabitation of the Marshall Islands. It is recommended that this study should be continued for at least two to three years in order to more accurately identify trends in local food consumption and living patterns.

  5. The Energy Institute Live Green, Burn Clean

    E-Print Network [OSTI]

    Lee, Dongwon

    The Energy Institute Live Green, Burn Clean: Advancing Engines for Renewable Fuels Live Green, Burn Clean: Advancing Engines for Renewable Fuels André Boehman Professor of Fuel Science and Materials College of Earth and Mineral Sciences The Pennsylvania State University André Boehman Professor of Fuel

  6. s Earth and environment s Living resources

    E-Print Network [OSTI]

    4 s Earth and environment s Living resources s Societies and health s Expertise and consulting of this trend is the acquisition, on a joint proposal from the Earth and Environment department and the Living phenomena so as to improve forecasting of the attendant hazards. The earth's crust: processes and natural

  7. Bioaugmentation of butane-utilizing microorganisms to promote cometabolism of 1,1,1-trichloroethane in groundwater microcosms

    E-Print Network [OSTI]

    Semprini, Lewis

    Bioaugmentation of butane-utilizing microorganisms to promote cometabolism of 1,1,1-trichloroethane. The initial inoculum for bioaugmentation was a butane-utilizing enrichment from the subsurface of the Hanford DOE site. The non-augmented microcosm required 80 days of incubation before butane

  8. WHAT IS COMPOST? Composting refers to the biological decomposition and stabilization of organic materials by microorganisms under

    E-Print Network [OSTI]

    Mukhtar, Saqib

    WHAT IS COMPOST? Composting refers to the biological decomposition and stabilization of organic materials by microorganisms under aerobic (in the presence of oxygen) conditions. During the composting, good quality compost is produced that is biologically stable, relatively uniform in appearance, free

  9. Spatially-Resolved Analysis of Glycolipids and Metabolites in Living Synechococcus sp. PCC7002 Using Nanospray Desorption Electrospray Ionization

    SciTech Connect (OSTI)

    Lanekoff, Ingela T.; Geydebrekht, Oleg V.; Pinchuk, Grigoriy E.; Konopka, Allan; Laskin, Julia

    2013-04-07

    Microorganisms release a diversity of organic compounds that couple interspecies metabolism, enable communication, or provide benefits to other microbes. Increased knowledge of microbial metabolite production will contribute to understanding of the dynamic microbial world and can potentially lead to new developments in drug discovery, biofuel production, and clinical research. Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that enables detailed chemical characterization of molecules from a specific location on a surface without special sample pretreatment. Due to its ambient nature, living bacterial colonies growing on agar plates can be rapidly and non-destructively analyzed. We performed spatially resolved nano-DESI analysis of living Synechococcus sp. PCC 7002 colonies on agar plates. We use high resolution mass spectrometry and MS/MS analysis of the living Synechococcus sp. PCC 7002 colonies to detect metabolites and lipids, and confirm their identities. We found that despite the high salt content of the agar (osmolarity ca. 700 mM), nano-DESI analysis enables detailed characterization of metabolites produced by the colony. Using this technique, we identified several glycolipids found on the living colonies and examined the effect of the age of the colony on the chemical gradient of glucosylglycerol secreted onto agar.

  10. Living Rev. Solar Phys., 10, (2013), 1 http://www.livingreviews.org/lrsp-2013-1

    E-Print Network [OSTI]

    Usoskin, Ilya G.

    2013-01-01

    Living Rev. Solar Phys., 10, (2013), 1 http://www.livingreviews.org/lrsp-2013-1 (Update of lrsp-2008-3) in solar physics L I V I N G REVIEWS A History of Solar Activity over Millennia Ilya G. Usoskin is a review of present knowledge of the long-term behavior of solar activity on a multi-millennial timescale

  11. Natural analogue studies of the role of colloids, natural organics and microorganisms on radionuclide transport

    SciTech Connect (OSTI)

    McCarthy, J.F.

    1994-10-01

    Colloids may be important as a geochemical transport mechanism for radionuclides at geological repositories if they are (1) present in the groundwater, (2) stable with respect to both colloidal and chemical stabilities, (3) capable of adsorbing radionuclides, especially if the sorption is irreversible, and (4) mobile in the subsurface. The available evidence from natural analogue and other field studies relevant to these issues is reviewed, as is the potential role of mobile microorganisms ({open_quotes}biocolloids{close_quotes}) on radionuclide migration. Studies have demonstrated that colloids are ubiquitous in groundwater, although colloid concentrations in deep, geochemically stable systems may be too low to affect radionuclide transport. However, even low colloid populations cannot be dismissed as a potential concern because colloids appear to be stable, and many radionuclides that adsorb to colloids are not readily desorbed over long periods. Field studies offer somewhat equivocal evidence concerning colloid mobility and cannot prove or disprove the significance of colloid transport in the far-field environment. Additional research is needed at new sites to properly represent a repository far-field. Performance assessment would benefit from natural analogue studies to examine colloid behavior at sites encompassing a suite of probable groundwater chemistries and that mimic the types of formations selected for radioactive waste repositories.

  12. Bioengineering and Coordination of Regulatory Networks and Intracellular Complexes to Maximize Hydrogen Production by Phototrophic Microorganisms

    SciTech Connect (OSTI)

    Tabita, F. Robert [The Ohio State University] [The Ohio State University

    2013-07-30

    In this study, the Principal Investigator, F.R. Tabita has teemed up with J. C. Liao from UCLA. This project's main goal is to manipulate regulatory networks in phototrophic bacteria to affect and maximize the production of large amounts of hydrogen gas under conditions where wild-type organisms are constrained by inherent regulatory mechanisms from allowing this to occur. Unrestrained production of hydrogen has been achieved and this will allow for the potential utilization of waste materials as a feed stock to support hydrogen production. By further understanding the means by which regulatory networks interact, this study will seek to maximize the ability of currently available “unrestrained” organisms to produce hydrogen. The organisms to be utilized in this study, phototrophic microorganisms, in particular nonsulfur purple (NSP) bacteria, catalyze many significant processes including the assimilation of carbon dioxide into organic carbon, nitrogen fixation, sulfur oxidation, aromatic acid degradation, and hydrogen oxidation/evolution. Moreover, due to their great metabolic versatility, such organisms highly regulate these processes in the cell and since virtually all such capabilities are dispensable, excellent experimental systems to study aspects of molecular control and biochemistry/physiology are available.

  13. Colonial Invasions, Colonial Lives History 302

    E-Print Network [OSTI]

    Fletcher, Robin

    1 Colonial Invasions, Colonial Lives History 302 Consider Resources Primary Resources: diaries (scholarly vs popular), theses, the Web... Check subject guide under: Research by Subject History Library Reference collection unless otherwise indicated. Encyclopedia of Cuba: People, History, Culture

  14. campus as living laboratory institutional Sustainability Plan

    E-Print Network [OSTI]

    Calgary, University of

    operations campus as living laboratory diversity campus waste institutional Sustainability Plan eliminate priorities energy research 35%ofwastedivertedSTARSSilver 275 sustainability courses investment 60 accessibility faculty leadership 100+ sustainability research projects over $3.8 million in bursaries Calgary

  15. The Live Room : transducing resonant architectures

    E-Print Network [OSTI]

    Bain, Mark, 1966-

    1998-01-01

    The Live Room is a temporary site specific installation presented in building N 51, room 117 on the MIT campus on May 7, 1998 and concluded on June 10, 1998. Using small acoustic intensifying equipment which mount directly ...

  16. UPC: HNB 100 LIVE Hedco Neurosciences Building

    E-Print Network [OSTI]

    Valero-Cuevas, Francisco

    UPC: HNB 100 ­ LIVE Hedco Neurosciences Building UPC Campus Map/Directions: http://www.usc.edu/about/visit/upc/ HSC: CHP 147 -- Video Conference Center for the Health Professional HSC Campus Map/Directions: http

  17. Creating an inspiring and sustainable living

    E-Print Network [OSTI]

    Langendoen, Koen

    to shape and plan for more sustainable development. Specialisations: · Urban TransformationsCreating an inspiring and sustainable living environment Diploma Master of Science Architecture, Urbanism and Building Sciences Credits 120 ECTS, 24 months Start BSc Bouwkunde graduates can enrol monthly

  18. Dynamical real numbers and living systems

    E-Print Network [OSTI]

    Dhurjati Prasad Datta

    2010-01-11

    Recently uncovered second derivative discontinuous solutions of the simplest linear ordinary differential equation define not only an nonstandard extension of the framework of the ordinary calculus, but also provide a dynamical representation of the ordinary real number system. Every real number can be visualized as a living cell -like structure, endowed with a definite evolutionary arrow. We discuss the relevance of this extended calculus in the study of living systems. We also present an intelligent version of the Newton's first law of motion.

  19. Ultrahigh-Resolution Magnetic Resonance in Inhomogeneous Magnetic Fields: Two-Dimensional Long-Lived-Coherence Correlation Spectroscopy

    E-Print Network [OSTI]

    sophisticated gradient switching or frequency-modulated pulses. Applications to in-cell NMR and to magnetic, metabolism, blood flow, diffusion, and brain activity. Most applications to inanimate samples and living

  20. Assessment of Parasitic Activity of Fusarium Strains Obtained from a Heterodera schachtii-Suppressive Soil

    E-Print Network [OSTI]

    Gao, Xuebiao; Yin, Bei; Borneman, James; Becker, J. Ole

    2008-01-01

    presence of pathogen- antagonistic microorganisms and haveIdentifying microorganisms involved in speci?c pathogen

  1. Biodesulfurization techniques: Application of selected microorganisms for organic sulfur removal from coals. Final report

    SciTech Connect (OSTI)

    Elmore, B.B.

    1993-08-01

    As an alternative to post-combustion desulfurization of coal and pre-combustion desulfurization using physicochemical techniques, the microbial desulfurization of coal may be accomplished through the use of microbial cultures that, in an application of various microbial species, may remove both the pyritic and organic fractions of sulfur found in coal. Organisms have been isolated that readily depyritize coal but often at prohibitively low rates of desulfurization. Microbes have also been isolated that may potentially remove the organic-sulfur fraction present in coal (showing promise when acting on organic sulfur model compounds such as dibenzothiophene). The isolation and study of microorganisms demonstrating a potential for removing organic sulfur from coal has been undertaken in this project. Additionally, the organisms and mechanisms by which coal is microbially depyritized has been investigated. Three cultures were isolated that grew on dibenzothiophene (DBT), a model organic-sulfur compound, as the sole sulfur source. These cultures (UMX3, UMX9, and IGTS8) also grew on coal samples as the sole sulfur source. Numerous techniques for pretreating and ``cotreating`` coal for depyritization were also evaluated for the ability to improve the rate or extent of microbial depyritization. These include prewashing the coal with various solvents and adding surfactants to the culture broth. Using a bituminous coal containing 0.61% (w/w) pyrite washed with organic solvents at low slurry concentrations (2% w/v), the extent of depyritization was increased approximately 25% in two weeks as compared to controls. At slurry concentrations of 20% w/v, a tetrachloroethylene treatment of the coal followed by depyritization with Thiobacillus ferrooxidans increased both the rate and extent of depyritization by approximately 10%.

  2. Metallomics of two microorganisms relevant to heavy metal bioremediation reveal fundamental differences in metal assimilation and utilization

    SciTech Connect (OSTI)

    Lancaster, Andrew [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Menon, Angeli [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Scott, Israel [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Poole, Farris [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Vaccaro, Brian [University of Georgia, Athens, GA] [University of Georgia, Athens, GA; Thorgersen, Michael P [ORNL] [ORNL; Geller, Jil [Lawrence Berkeley National Laboratory (LBNL)] [Lawrence Berkeley National Laboratory (LBNL); Hazen, Terry C [ORNL] [ORNL; Hurt, Jr., Richard Ashley [ORNL; Brown, Steven D [ORNL] [ORNL; Elias, Dwayne A [ORNL] [ORNL; Adams, Michael W. W. [University of Georgia, Athens, GA] [University of Georgia, Athens, GA

    2014-01-01

    Although as many as half of all proteins are thought to require a metal cofactor, the metalloproteomes of microorganisms remain relatively unexplored. Microorganisms from different environments are likely to vary greatly in the metals that they assimilate, not just among the metals with well-characterized roles but also those lacking any known function. Herein we investigated the metal utilization of two microorganisms that were isolated from very similar environments and are of interest because of potential roles in the immobilization of heavy metals, such as uranium and chromium. The metals assimilated and their concentrations in the cytoplasm of Desulfovibrio vulgaris strain Hildenborough (DvH) and Enterobacter cloacae strain Hanford (EcH) varied dramatically, with a larger number of metals present in Enterobacter. For example, a total of 9 and 19 metals were assimilated into their cytoplasmic fractions, respectively, and DvH did not assimilate significant amounts of zinc or copper whereas EcH assimilated both. However, bioinformatic analysis of their genome sequences revealed a comparable number of predicted metalloproteins, 813 in DvH and 953 in EcH. These allowed some rationalization of the types of metal assimilated in some cases (Fe, Cu, Mo, W, V) but not in others (Zn, Nd, Ce, Pr, Dy, Hf and Th). It was also shown that U binds an unknown soluble protein in EcH but this incorporation was the result of extracellular U binding to cytoplasmic components after cell lysis.

  3. Senior Adviser Richard Kauffman to Host Live Chat on Renewable...

    Office of Environmental Management (EM)

    Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and Deployment Senior Adviser Richard Kauffman to Host Live Chat on Renewable Energy Innovation and...

  4. University Teams to Showcase Affordable, Energy Efficient Living...

    Office of Environmental Management (EM)

    University Teams to Showcase Affordable, Energy Efficient Living in U.S. Department of Energy Solar Decathlon 2011 University Teams to Showcase Affordable, Energy Efficient Living...

  5. Siphon-based characterization of liveness and liveness-enforcing supervision

    E-Print Network [OSTI]

    Reveliotis, Spiridon "Spyros"

    1 Siphon-based characterization of liveness and liveness-enforcing supervision for sequential of empty, or more generally, deadly marked siphon. The work presented in this paper seeks to develop ­ and in certain cases, empty ­ siphon. In this capacity, the pre- sented results allow also the extension

  6. INTRODuCTION PrOtectiNg Lives ANd LiveLihOOds

    E-Print Network [OSTI]

    i-3 INTRODuCTION NOAA: PrOtectiNg Lives ANd LiveLihOOds NOAA provides weather, water, and climate safe, efficient, and secure transportation on U.S. waterways. The U.S. Marine Transporta- tion System Management Act, National Estuarine Research Reserves, and National Marine Sanctuaries. NOAA also supports

  7. Working at Living: The Social Relations of Precarity

    E-Print Network [OSTI]

    Boris, Eileen; Dodson, Leigh

    2013-01-01

    and  depth.  They  considered   working  at  living  through  home  labors,  transnational  and  national  migration,  

  8. Student HouSing BenefitS of Living

    E-Print Network [OSTI]

    Matrajt, Graciela

    Student HouSing #12;BenefitS of Living in Student HouSing · Live with your peers in an exciting with housing costs for eligible students · Onsite parking at housing lots · Living Learning Communities the leadership of these units, students will enjoy the usual perks of living in campus housing, but will also

  9. Brisbane 2011: Living with floods and

    E-Print Network [OSTI]

    Boynton, Walter R.

    Brisbane 2011: Living with floods and dancing with dugongs 07 July 2011 Customs House University of Queensland Bill Dennison #12;Outline ·! Queensland floods = societal learning moment ·! Global lessons about flood responses ·! Conservation icons and charismatic ecosystems ·! Sustainability models for the future

  10. Living up to standards Margaret King

    E-Print Network [OSTI]

    to record my thanks to Nigel Bevan, technical editor of the ISO standards discussed for much interestingLiving up to standards Margaret King TIM/ISSCO ETI University of Geneva Margaret reusable" by arguing that a set of ISO standards developed for the evaluation of software in general

  11. Quality health plans & benefits Healthier living

    E-Print Network [OSTI]

    Zhigilei, Leonid V.

    Quality health plans & benefits Healthier living Financial well-being Intelligent solutions Do the Math! 2015-2016 University of Virginia Student Health Insurance Plan www.aetnastudenthealth.com 15.03.491.1 #12;A Student Health Insurance Plan may be the best alternative and here's why: A student health

  12. Long-lived tendrils viscous entrainment

    E-Print Network [OSTI]

    Gardel, Margaret

    Long-lived tendrils in viscous entrainment Wendy W. Zhang Laura E. Schmidt* Physics & James Franck entrained by warm upwards flow in thermal boundary layer ·Entrainment stabilizes upwards flow Kumagai et al-layer liquid entrained as steady spout Cohen & Nagel 2002 Phys. Rev. Lett. Cohen 2004 Phys. Rev. E Kleine

  13. Cooperative self-organization of microorganisms ESHEL BEN-JACOB, INON COHEN

    E-Print Network [OSTI]

    Jacob, Eshel Ben

    of movement according to gradient of chemical agent) signalling, collective activation and deactivation movement and chemotaxis 440 4.3. Bacterial metabolism and growth 442 4.4. Molecular genetics 443 4.5. From

  14. The effects of marine microorganisms on the mechanical properties of graphite/epoxy composites 

    E-Print Network [OSTI]

    Puh, John Shui-Ming

    1997-01-01

    with two different lay-ups were conditioned in natural seawater and then tensile tested while simultaneously monitored for acoustic emission activity. Graphite/epoxy composite specimens were fabricated from prepreg tape and then conditioned for 4 and I I...

  15. Living Rev. Solar Phys., 5, (2008), 3 http://www.livingreviews.org/lrsp-2008-3 in solar physics

    E-Print Network [OSTI]

    Usoskin, Ilya G.

    2008-01-01

    Living Rev. Solar Phys., 5, (2008), 3 http://www.livingreviews.org/lrsp-2008-3 in solar physics L I V I N G REVIEWS A History of Solar Activity over Millennia Ilya G. Usoskin Sodankyl¨a Geophysical here is a review of present knowledge of the long-term behavior of solar activity on a multi

  16. Fission barriers and half-lives

    SciTech Connect (OSTI)

    Moeller, P.; Nix, J.R.; Swiatecki, W.J.

    1989-01-01

    We briefly review the development of theoretical models for the calculation of fission barriers and half-lives. We focus on how results of actual calculations in a unified macroscopic-microscopic approach provide an interpretation of the mechanisms behind some of the large number of phenomena observed in fission. As instructive examples we choose studies of the rapidly varying fission properties of elements at the end of the periodic system. 31 refs., 10 figs.

  17. South Atlantic OCS area living marine resources study. Volume II: an investigation of live bottom habitats north of Cape Fear, North Carolina. Final report

    SciTech Connect (OSTI)

    Not Available

    1981-10-01

    The major objectives of this study were to (1) characterize benthic and nektonic communities associated with representative live bottom habitats on the continental shelf of the South Atlantic Bight, and (2) evaluate factors which might influence these communities, particularly the potential for impact by offshore oil and gas activities. The report describes three study sites at the edge of the continental shelf in a 55-100m depth zone, near Cape Fear, North Carolina.

  18. Living Comfortably: A Consumer's Guide to Home Energy Upgrades...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Living Comfortably: A Consumer's Guide to Home Energy Upgrades Living Comfortably: A Consumer's Guide to Home Energy Upgrades March 7, 2013 - 3:15pm Addthis A weatherization worker...

  19. Solar Decathlon Team Leading the Way Toward Sustainable Living...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of Disasters Solar Decathlon Team Leading the Way Toward Sustainable Living, Even in the Wake of...

  20. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced Biofuels Acting Biomass Program Manager Dr. Valerie Reed to Host Live Twitter Q&A on Advanced...

  1. Morgantown Slightly Exceeds National Average for Cost of Living

    E-Print Network [OSTI]

    Mohaghegh, Shahab

    (an index value of 100 reflects the national average). The index expresses the cost of living, health care, and miscellaneous goods and services. The index is designed to reflect the cost of living Relative to National Average by Category In Figure 2, we illustrate how the cost of living index has

  2. Hierarchical classification with reject option for live fish recognition

    E-Print Network [OSTI]

    Fisher, Bob

    Hierarchical classification with reject option for live fish recognition Phoenix X. Huang@inf.ed.ac.uk Robert B. Fisher University of Edinburgh rbf@inf.ed.ac.uk Abstract A live fish recognition system. We present a novel Balance-Enforced Optimized Tree with Reject op- tion (BEOTR) for live fish

  3. Proton radioactivity half lives with Skyrme interactions

    E-Print Network [OSTI]

    T. R. Routray; Abhishek Mishra; S. K. Tripathy; B. Behera; D. N. Basu

    2012-05-31

    The potential barrier impeding the spontaneous emission of protons in the proton radioactive nuclei is calculated as the sum of nuclear, Coulomb and centrifugal contributions. The nuclear part of the proton-nucleus interaction potential is obtained in the energy density formalism using Skyrme effective interaction that results into a simple algebraic expression. The half-lives of the proton emitters are calculated for the different Skyrme sets within the improved WKB framework. The results are found to be in reasonable agreement with the earlier results obtained for more complicated calculations involving finite range interactions.

  4. Safety assessment of outdoor live fire range

    SciTech Connect (OSTI)

    1989-05-01

    The following Safety Assessment (SA) pertains to the outdoor live fire range facility (LFR). The purpose of this facility is to supplement the indoor LFR. In particular it provides capacity for exercises that would be inappropriate on the indoor range. This SA examines the risks that are attendant to the training on the outdoor LFR. The outdoor LFR used by EG&G Mound is privately owned. It is identified as the Miami Valley Shooting Grounds. Mondays are leased for the exclusive use of EG&G Mound.

  5. Solar Living Institute | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Information Serbia-Enhancing Capacity forSilicium deEnergy InformationDepotGreen TechnologyLiving

  6. Living Walls | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LISTStar2-0057-EAInvervarLeeds, UnitedLibertyLite On TechnologyCornLiuzhouLiving Walls

  7. Women @ Energy: Kelly Lively | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirleyEnergyThe U.S.Lacledeutilities.Energy ThefullAssociateJenniferCarradoKelly Lively has

  8. Live pathogens: rapid detection technique developed

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenterLee FacultyadivisionLive

  9. Influence of microorganisms on the oxidation state distribution of multivalent actinides under anoxic conditions

    SciTech Connect (OSTI)

    Reed, Donald Timothy [Los Alamos National Laboratory; Borkowski, Marian [Los Alamos National Laboratory; Lucchini, Jean - Francois [Los Alamos National Laboratory; Ams, David [Los Alamos National Laboratory; Richmann, M. K. [Los Alamos National Laboratory; Khaing, H. [Los Alamos National Laboratory; Swanson, J. S. [Los Alamos National Laboratory

    2010-12-10

    The fate and potential mobility of multivalent actinides in the subsurface is receiving increased attention as the DOE looks to cleanup the many legacy nuclear waste sites and associated subsurface contamination. Plutonium, uranium and neptunium are the near-surface multivalent contaminants of concern and are also key contaminants for the deep geologic disposal of nuclear waste. Their mobility is highly dependent on their redox distribution at their contamination source as well as along their potential migration pathways. This redox distribution is often controlled, especially in the near-surface where organic/inorganic contaminants often coexist, by the direct and indirect effects of microbial activity. Under anoxic conditions, indirect and direct bioreduction mechanisms exist that promote the prevalence of lower-valent species for multivalent actinides. Oxidation-state-specific biosorption is also an important consideration for long-term migration and can influence oxidation state distribution. Results of ongoing studies to explore and establish the oxidation-state specific interactions of soil bacteria (metal reducers and sulfate reducers) as well as halo-tolerant bacteria and Archaea for uranium, neptunium and plutonium will be presented. Enzymatic reduction is a key process in the bioreduction of plutonium and uranium, but co-enzymatic processes predominate in neptunium systems. Strong sorptive interactions can occur for most actinide oxidation states but are likely a factor in the stabilization of lower-valent species when more than one oxidation state can persist under anaerobic microbiologically-active conditions. These results for microbiologically active systems are interpreted in the context of their overall importance in defining the potential migration of multivalent actinides in the subsurface.

  10. Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1,1,1-trichloroethane and 1,1-dichloroethene

    E-Print Network [OSTI]

    Semprini, Lewis

    Bioaugmentation with butane-utilizing microorganisms to promote in situ cometabolic treatment of 1) through bioaugmentation with a butane enrichment culture containing predominantly two Rhodococcus sp of butane and dissolved oxygen and or hydrogen peroxide as sources of dissolved oxygen, about 70% removal

  11. Marine microorganisms dominate the ocean ecosystem and sustain planetary habitability. The scientists and educators on the C-MORE Team --women and

    E-Print Network [OSTI]

    Marine microorganisms dominate the ocean ecosystem and sustain planetary habitability to a unique partnership aimed at exploring and understanding how the marine microbial world is structured`i Partnership #12;Marine Microbiology Initiative VIA SEA MAIL SCHOOL OF OCEAN AND EARTH SCIENCE AND TECHNOLOGY U

  12. Fluorescence technique for on-line monitoring of state of hydrogen-producing microorganisms

    DOE Patents [OSTI]

    Seibert, Michael (Lakewood, CO); Makarova, Valeriya (Golden, CO); Tsygankov, Anatoly A. (Pushchino, RU); Rubin, Andrew B. (Moscow, RU)

    2007-06-12

    In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H.sub.2 under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H.sub.2 percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H.sub.2 data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in F.sub.t (steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in F.sub.m', (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in .DELTA.F/F.sub.m'=(F.sub.m'-F.sub.t)/F.sub.m' (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H.sub.2 production that signal oxidation of plastoquinone pool asmain factor to regulate H.sub.2 under sulfur depletion.

  13. Living and Working in the Freezer

    SciTech Connect (OSTI)

    Hill, Victoria (Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity) [Dept of Ocean, Earth, and Atmospheric Sciences, Old Dominion Unversity

    2012-02-07

    Very little data of any kind exists from the early spring in the Arctic. The reason? It's extremely cold and that makes it difficult to survive, let alone conduct science. From March through the end of April, 2011, scientists from around the world braved temperatures of -48?C in the high Canadian Arctic in the name of science. At the Catlin Arctic Survey's floating 'Ice Base' off Ellef Ringnes Island, Dr. Victoria Hill was investigating how organic material in fresh water near the surface of the ocean may be trapping heat from the sun, causing the upper ocean layers to warm. This is a very new area of research and this mechanism represents a key uncertainty in accurate modeling of ice thickness and upper ocean heat content. In this presentation Dr. Hill will talk about living and working at the ice base and discuss preliminary data from the expedition.

  14. Apparatus and method for transforming living cells

    DOE Patents [OSTI]

    Okandan, Murat; Galambos, Paul C.

    2003-11-11

    An apparatus and method are disclosed for in vitro transformation of living cells. The apparatus, which is formed as a microelectromechanical device by surface micromachining, can be used to temporarily disrupt the cell walls or membrane of host cells one at a time so that a particular substance (e.g. a molecular tag, nucleic acid, bacteria, virus etc.) can be introduced into the cell. Disruption of the integrity of the host cells (i.e. poration) can be performed mechanically or electrically, or by both while the host cells are contained within a flow channel. Mechanical poration is possible using a moveable member which has a pointed or serrated edge and which is driven by an electrostatic actuator to abrade, impact or penetrate the host cell. Electroporation is produced by generating a relatively high electric field across the host cell when the host cell is located in the flow channel between a pair of electrodes having a voltage applied therebetween.

  15. Dynamic Metabolism Studies of Live Bacterial Films

    SciTech Connect (OSTI)

    Majors, Paul D.; Mclean, Jeffrey S.

    2008-11-01

    Bacterial film (biofilm) microbes exist within spatial (nutrient, electron-acceptor, pH, etc.) gradients of their own making. Correspondingly, biofilm bacteria are physiologically and functionally distinct from free-floating bacteria and from their own species at differing biofilm depths. This article describes our efforts to develop noninvasive nuclear magnetic resonance (NMR) technologies for biofilm-metabolism studies. This involves integrating NMR with controlled-cultivation methods to interrogate microbial physiology live and under known growth conditions. NMR is uniquely capable of providing depth-resolved metabolic and transport information in a non-invasive, non-sample-consuming fashion, providing information required for experimental reactive transport studies. We have studied mono-species biofilms relevant to environment remediation and human health. We describe these technologies, discuss their advantages and limitations, and give examples of their application.

  16. AALO: Activity recognition in smart homes using Active Learning in the presence of Overlapped

    E-Print Network [OSTI]

    Weimer, Westley

    (HMM and HSMM). I. INTRODUCTION Due to increasing number of elderly people and single households living activities are instrumented with passive sensors. When a resident moves from one room to another or uses

  17. Slow urban living apartments : transformation of five story walk-up apartments in Seoul

    E-Print Network [OSTI]

    Lim, Yihyun

    2014-01-01

    Experiential living is the new trend for future living. Whether it is through living in micro spaces, flexible units, mixed-use developments, practicing urban farming, or sharing lifestyles, these different trends of living ...

  18. 4-H Activities 

    E-Print Network [OSTI]

    Howard, Jeff W.

    2005-05-10

    ? are heard often when 4-H members work and learn together. Preparing and presenting demonstrations and illustrated talks help 4-H members gain additional knowl- edge and learn new skills and practices. Younger mem- bers start with easy-to-do ?show and tell... decisions ver- bally. ? Tolerant of other?s decisions. ? Use group activities to increase their interest in 4-H club work. ? Apply what was learned through judging in everyday living. Community Service Community service activities enable 4-H mem- bers...

  19. Live Plant Sample c/o Rob Richardson

    E-Print Network [OSTI]

    Krings, Alexander

    and degrade. 6. Next day or priority mail is recommended for shipping fresh samples. Be sure package is labeled with "Live Plant" #12;

  20. Brownsville Public Utilities Board- Green Living Residential Rebate Program

    Broader source: Energy.gov [DOE]

     Brownsville Public Utilities Board offers residential customers rebates for installation of energy efficient measures. Through the Green Living Rebate program, customers can apply for rebates for...

  1. Live, Audio-Visual Communication Systems for Distance Learning

    E-Print Network [OSTI]

    Finkelstein, Anthony

    , WC1H 0AP, U.K. tel: (44) (0)71 387 7050 ext. 5315 fax: (44) (0)71 580 1100 #12;Submission Live, Audio

  2. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. [Brookhaven National Lab., Upton, NY (United States); Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)

    1993-08-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 108 have been compiled and evaluated. Recommended values are presented.

  3. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    protein phosphorylation inside living mammalian cells, enabling them to follow cellular chemical changes in real time, without bias. Advancing Medicine with Infrared Light...

  4. An ultra-sensitive microfluidic immunoassay using living radical...

    Office of Scientific and Technical Information (OSTI)

    microfluidic immunoassay using living radical polymerization and porous polymer monoliths. Citation Details In-Document Search Title: An ultra-sensitive microfluidic...

  5. Virtual Institute of Microbial Stress and Survival: Deduction of Stress Response Pathways in Metal and Radionuclide Reducing Microorganisms

    SciTech Connect (OSTI)

    2004-04-17

    The projects application goals are to: (1) To understand bacterial stress-response to the unique stressors in metal/radionuclide contamination sites; (2) To turn this understanding into a quantitative, data-driven model for exploring policies for natural and biostimulatory bioremediation; (3) To implement proposed policies in the field and compare results to model predictions; and (4) Close the experimental/computation cycle by using discrepancies between models and predictions to drive new measurements and construction of new models. The projects science goals are to: (1) Compare physiological and molecular response of three target microorganisms to environmental perturbation; (2) Deduce the underlying regulatory pathways that control these responses through analysis of phenotype, functional genomic, and molecular interaction data; (3) Use differences in the cellular responses among the target organisms to understand niche specific adaptations of the stress and metal reduction pathways; (4) From this analysis derive an understanding of the mechanisms of pathway evolution in the environment; and (5) Ultimately, derive dynamical models for the control of these pathways to predict how natural stimulation can optimize growth and metal reduction efficiency at field sites.

  6. Why we live in 3 Dimensions

    E-Print Network [OSTI]

    Carlos Castro; Alex Granik; M. S. El Naschie

    2000-08-18

    A Cantorian fractal spacetime, a family member of von Neumann's noncommutative geometry is introduced as a geometry underlying a new relativity theory which is similar to the relation between general relativity and Riemannian geometry. Based on this model and the new relativity theory an ensemble distribution of all the dimensions of quantum spacetime is derived with the help of Fermat grand theorem. The calculated average dimension is very close to the value of $4+\\phi^3 $ (where $\\phi$ is the golden mean) obtained by El Naschie on the basis of a different approach. It is shown that within the framework of the new relativity the cosmological constant problem is nonexistent, since the Universe self-organizes and self-tunes according to the renormalization group (RG) flow with respect to a local scaling microscopic arrow of time. This implies that the world emerged as a result of a non-equilibrium process of self-organized critical phenomena launched by vacuum fluctuations in Cantorian fractal spacetime $\\cal E^{\\infty}$. It is shown that we are living in a metastable vacuum and are moving towards a fixed point ($ D$ = 4+$\\phi^3$) of the RG. After reaching this point, a new phase transition will drive the universe to a quasi-crystal phase of the lower average dimension of $\\phi^3$.

  7. Long Lived Fourth Generation and the Higgs

    E-Print Network [OSTI]

    Wai-Yee Keung; Pedro Schwaller

    2011-05-31

    A chiral fourth generation is a simple and well motivated extension of the standard model, and has important consequences for Higgs phenomenology. Here we consider a scenario where the fourth generation neutrinos are long lived and have both a Dirac and Majorana mass term. Such neutrinos can be as light as 40 GeV and can be the dominant decay mode of the Higgs boson for Higgs masses below the W-boson threshold. We study the effect of the Majorana mass term on the Higgs branching fractions and reevaluate the Tevatron constraints on the Higgs mass. We discuss the prospects for the LHC to detect the semi-invisible Higgs decays into fourth generation neutrino pairs. Under the assumption that the lightest fourth generation neutrino is stable, it's thermal relic density can be up to 20% of the observed dark matter density in the universe. This is in agreement with current constraints on the spin dependent neutrino-neutron cross section, but can be probed by the next generation of dark matter direct detection experiments.

  8. Electromicroinjection of particles into living cells

    DOE Patents [OSTI]

    Ray, F. Andrew (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM); Galey, William R. (Albuquerque, NM)

    1988-01-01

    Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge the particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.

  9. The purpose of NOAA's Weather-Ready Nation initiative is first and foremost to save lives and

    E-Print Network [OSTI]

    The purpose of NOAA's Weather-Ready Nation initiative is first and foremost to save lives public. The Weather-Ready Nation initiative will help America meet the challenges that come with increased extreme weather events by more actively engaging the public on preparedness and resiliency. What

  10. Shybot: Friend-Stranger Interaction for Children Living with Autism

    E-Print Network [OSTI]

    Shybot: Friend-Stranger Interaction for Children Living with Autism Abstract This paper presents this simple social interaction to open up a new direction for intervention for children living with autism. We hope that from minimal social interaction, a child with autism or social anxiety disorders could

  11. Percolation Approach to Study Connectivity in Living Neural Networks

    E-Print Network [OSTI]

    Moses, Elisha

    Percolation Approach to Study Connectivity in Living Neural Networks Jordi Soriano, Ilan Breskin distribution and not a power law one. Keywords: neural networks, graphs, connectivity, percolation, giant as the fundamental feature to understand the potential of a living neural network. Unravelling the detailed

  12. Root Modeling: Estimating Storage, Live, and Dead Pool

    E-Print Network [OSTI]

    Post, Wilfred M.

    Root Modeling: Estimating Storage, Live, and Dead Pool Turnover Times; Storage Inputs to New Root to choose best-fit parameters · "Storage" simulations with in-growth cores · Live and dead pool simulations Atmosphere East Atmosphere; 1 SD East tree rings f(Average respiration and soil gas) Modeled GSD = 1.3 Range

  13. Carbon nanotubes as photoacoustic molecular imaging agents in living mice

    E-Print Network [OSTI]

    Khuri-Yakub, Butrus T. "Pierre"

    Carbon nanotubes as photoacoustic molecular imaging agents in living mice ADAM DE LA ZERDA1 not shown to target a diseased site in living subjects. Here we show that single-walled carbon nanotubes were verified ex vivo using Raman microscopy. Photoacoustic imaging of targeted single-walled carbon

  14. Long Lived Charged Massive Particles and Big Bang Nucleosynthesis

    E-Print Network [OSTI]

    Kazunori Kohri; Fumihiro Takayama

    2006-11-06

    We consider Big Bang Nucleosynthesis(BBN) with long lived charged massive particles. Before decaying, the long lived massive particles recombines with a light element to form a bound state like a hydrogen atom. We discuss the possible change of primordial light element abundances due to formations of such bound states.

  15. Rocky Flats Plant Live-Fire Range Risk Analysis Report

    SciTech Connect (OSTI)

    Nicolosi, S.L.; Rodriguez, M.A.

    1994-04-01

    The objective of the Live-Fire Range Risk Analysis Report (RAR) is to provide an authorization basis for operation as required by DOE 5480.16. The existing Live-Fire Range does not have a safety analysis-related authorization basis. EG&G Rocky Flats, Inc. has worked with DOE and its representatives to develop a format and content description for development of an RAR for the Live-Fire Range. Development of the RAR is closely aligned with development of the design for a baffle system to control risks from errant projectiles. DOE 5480.16 requires either an RAR or a safety analysis report (SAR) for live-fire ranges. An RAR rather than a SAR was selected in order to gain flexibility to more closely address the safety analysis and conduct of operation needs for a live-fire range in a cost-effective manner.

  16. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekov, Andrei D. (Moscow, RU)

    2007-10-30

    The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  17. Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

    DOE Patents [OSTI]

    Bavykin, Sergei G. (Darien, IL); Mirzabekova, legal representative, Natalia V. (Westmont, IL); Mirzabekov, deceased, Andrei D. (Westmont, IL)

    2007-12-04

    The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

  18. Mechanisms Regulating Selective Gene Activation During the Innate Immune Response

    E-Print Network [OSTI]

    Tong, Ann-Jay

    2015-01-01

    elements found on microorganisms. These pathogen-associatedpathogen is the generation of a suitable immune response, as defense against an extracellular microorganism

  19. Genetically modified microorganisms for

    E-Print Network [OSTI]

    Angenent, Lars T.

    of our understanding of microbial reactions in microbial fuel cells (MFCs), or more generally to tailor these microbial reactions to optimize the desired functionality. We are still in the infancy

  20. Radiation-resistant microorganism

    DOE Patents [OSTI]

    Fliermans, Carl B.

    2010-06-15

    An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.

  1. Student Activities Student Activities

    E-Print Network [OSTI]

    with Solar Energy? Activity B How do Atmospheres Produce their Effect Upon Surface Temperatures? Activity C and populations found in developing countries in Asia, Africa and Latin America. Figure 4.3 illustrates the actual and projected growths in global population. 0Topic ,Real World Problem: Culprits of Climate Warming and Cooling

  2. Generation of Long-Lived Isomeric States via Bremsstrahlung Irradiation

    E-Print Network [OSTI]

    Yao Cheng; Bing Xia; Chuanxiang Tang; Yinong Liu; Qingxiu Jin

    2006-11-08

    A method to generate long-lived isomeric states effectively for Mossbauer applications is reported. We demonstrate that this method is better and easier to provide highly sensitive Mossbauer effect of long-lived isomers (>1ms) such as 103Rh. Excitation of (gamma,gamma) process by synchrotron radiation is painful due mainly to their limited linewidth. Instead,(gamma,gamma') process of bremsstrahlung excitation is applied to create these long-lived isomers. Isomers of 45Sc, 107Ag, 109Ag, and 103Rh have been generated from this method. Among them, 103Rh is the only one that we have obtained the gravitational effect at room temperature.

  3. Long lived central engines in Gamma Ray Bursts

    E-Print Network [OSTI]

    G. Ghisellini

    2008-10-01

    The central engine of Gamma Ray Bursts may live much longer than the duration of the prompt emission. Some evidence of it comes from the presence of strong precursors, post-cursors, and X-ray flares in a sizable fraction of bursts. Additional evidence comes from the fact that often the X-ray and the optical afterglow light curves do not track one another, suggesting that they are two different emission components. The typical "steep-flat-steep" behavior of the X-ray light curve can be explained if the same central engine responsible for the main prompt emission continues to be active for a long time, but with a decreasing power. The early X-ray "afterglow" emission is then the extension of the prompt emission, originating at approximately the same location, and is not due to forward shocks. If the bulk Lorentz factor Gamma is decreasing in time, the break ending the shallow phase can be explained, since at early times Gamma is large, and we see only a fraction of the emitting area. Later, when Gamma decreases, we see an increasing fraction of the emitting surface up to the time when Gamma ~ 1/theta_j. This time ends the shallow phase of the X-ray light curve. The origin of the late prompt emission can be the accretion of the fall-back material, with an accretion rate dot M proportional to t^(-5/3). The combination of this late prompt emission with the flux produced by the standard forward shock can explain the great diversity of the optical and the X-ray light curves.

  4. Continuum- based computational models of biological living cell 

    E-Print Network [OSTI]

    Cheng, Feifei

    2009-05-15

    All living creatures, despite their profound diversity, share a common architectural building block: the cell. Cells are the basic functional units of life, yet are themselves comprised of numerous components with distinct ...

  5. The living commons : a spatial theory for biological design

    E-Print Network [OSTI]

    Telhan, Orkan

    2013-01-01

    Biological design is as ancient as human civilization. For thousands of years, living systems and natural processes have been manipulated by humans and their biological outcomes have been customized for different purposes. ...

  6. Ask a scientist: Nanotech in our lives | Argonne National Laboratory

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Ask a scientist: Nanotech in our lives June 1, 2014 Tweet EmailPrint Is there nanotechnology already in my consumer products? Carrado Gregar: I just saw a report that named...

  7. Optimization of environmental control to fit living space requirements 

    E-Print Network [OSTI]

    Eckmann, Maxim S

    2013-02-22

    This study examines the application of Environmental Control Systems (ECSs) for people with disabilities who live in the dormitory. ECSs allow people with disabilities to control appliances in their homes and parameters ...

  8. Spontaneous fission half-lives for ground state nuclides

    SciTech Connect (OSTI)

    Holden, N.E. (Brookhaven National Lab., Upton, NY (United States)); Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States))

    1991-01-01

    Measurements of the spontaneous fission half-lives of nuclides of elements Z = 90 to 107 have been compiled and evaluated. Recommended values are presented. 126 refs., 96 tabs.

  9. University Housing Designing Sustainable Communities for Living and Learning

    E-Print Network [OSTI]

    Almor, Amit

    The Mission Learning Center for Sustainable Futures Green Learning Community Student Engagement Green Living Public Events Green Education University and Community Outreach Community Building Our Home:Physical Features LEED Certification Sustainable Sites Materials and Resources Energy and Atmosphere Indoor

  10. Live Webcast on Recent Wind Energy Technology Advances

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “Recent Wind Technology Advances” on April 16, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  11. Acting Biomass Program Manager Dr. Valerie Reed to Host Live...

    Broader source: Energy.gov (indexed) [DOE]

    December 16th, the Energy Department (@energy) will be hosting a live Twitter Q&A on biofuels with Dr. Valerie Reed, Acting Manager of the Biomass Program. Dr. Reed holds a Ph. D....

  12. Harnessing microbial subsurface metal reduction activities to synthesise nanoscale cobalt ferrite with enhanced magnetic properties

    SciTech Connect (OSTI)

    Coker, Victoria S.; Telling, Neil D.; van der Laan, Gerrit; Pattrick, Richard A.D.; Pearce, Carolyn I.; Arenholz, Elke; Tuna, Floriana; Winpenny, Richard E.P.; Lloyd, Jonathan R.

    2009-03-24

    Nanoscale ferrimagnetic particles have a diverse range of uses from directed cancer therapy and drug delivery systems to magnetic recording media and transducers. Such applications require the production of monodisperse nanoparticles with well-controlled size, composition, and magnetic properties. To fabricate these materials purely using synthetic methods is costly in both environmental and economical terms. However, metal-reducing microorganisms offer an untapped resource to produce these materials. Here, the Fe(III)-reducing bacterium Geobacter sulfurreducens is used to synthesize magnetic iron oxide nanoparticles. A combination of electron microscopy, soft X-ray spectroscopy, and magnetometry techniques was employed to show that this method of biosynthesis results in high yields of crystalline nanoparticles with a narrow size distribution and magnetic properties equal to the best chemically synthesized materials. In particular, it is demonstrated here that cobalt ferrite (CoFe{sub 2}O{sub 4}) nanoparticles with low temperature coercivity approaching 8 kOe and an effective anisotropy constant of {approx} 10{sup 6} erg cm{sup -3} can be manufactured through this biotechnological route. The dramatic enhancement in the magnetic properties of the nanoparticles by the introduction of high quantities of Co into the spinel structure represents a significant advance over previous biomineralization studies in this area using magnetotactic bacteria. The successful production of nanoparticulate ferrites achieved in this study at high yields could open up the way for the scaled-up industrial manufacture of nanoparticles using environmentally benign methodologies. Production of ferromagnetic nanoparticles for pioneering cancer therapy, drug delivery, chemical sensors, catalytic activity, photoconductive materials, as well as more traditional uses in data storage embodies a large area of inorganic synthesis research. In particular, the addition of transition metals other than Fe into the structure of magnetite (Fe{sub 3}O{sub 4}) has been shown to greatly enhance the magnetic properties of the particles, tailoring them to different commercial uses. However, synthesis of magnetic nanoparticles is often carried out at high temperatures with toxic solvents resulting in high environmental and energy costs. Additionally, these ferrite nanoparticles are not intrinsically biocompatible, and to make them suitable for insertion into the human body is a rather intricate task. A relatively unexplored resource for magnetic nanomaterial production is subsurface Fe(III)-reducing bacteria, as these microorganisms are capable of producing large quantities of nanoscale magnetite (Fe{sub 3}O{sub 4}) at ambient temperatures. Metal-reducing bacteria live in environments deficient in oxygen and conserve energy for growth through the oxidation of hydrogen or organic electron donors, coupled to the reduction of oxidized metals such as Fe(III)-bearing minerals. This can result in the formation of magnetite via the extracellular reduction of amorphous Fe(III)-oxyhydroxides causing the release of soluble Fe(II) and resulting in complete recrystallization of the amorphous mineral into a new phase. Some previous studies have reported altering the composition of biogenic magnetite produced by Fe(III)-reducing bacteria for industrial and environmental applications. However, research into the commercial exploitation of bacteria to form magnetic minerals has focused primarily on magnetotactic bacteria which form magnetosomal magnetite internally using very different pathways to those bacteria forming magnetite outside the cell. Magnetotactic bacteria live at the sediment-water interface and use internal nanomagnets to guide them to their preferred environmental niche using the Earth's magnetic field. Since magnetotactic bacteria generally grow optimally under carefully controlled microaerobic conditions, the culturing processes for these organisms are challenging and result in low yields of nanomagnetite. Despite these limitations, magnetotactic bacteria have bee

  13. Power Spectrum of Out-of-equilibrium Forces in Living Cells : Amplitude and Frequency Dependence

    E-Print Network [OSTI]

    Francois Gallet; Delphine Arcizet; Pierre Bohec; Alain Richert

    2009-01-20

    Living cells exhibit an important out-of-equilibrium mechanical activity, mainly due to the forces generated by molecular motors. These motor proteins, acting individually or collectively on the cytoskeleton, contribute to the violation of the fluctuation-dissipation theorem in living systems. In this work we probe the cytoskeletal out-of-equilibrium dynamics by performing simultaneous active and passive microrheology experiments, using the same micron-sized probe specifically bound to the actin cortex. The free motion of the probe exhibits a constrained, subdiffusive behavior at short time scales (t power law dependence with time. Combining the results of both experiments, we precisely measure for the first time the power spectrum of the force fluctuations exerted on this probe, which lies more than one order of magnitude above the spectrum expected at equilibrium, and greatly depends on frequency. We retrieve an effective temperature Teff of the system, as an estimate of the departure from thermal equilibrium. This departure is especially pronounced on long time scales, where Teff bears the footprint of the cooperative activity of motors pulling on the actin network. ATP depletion reduces the fluctuating force amplitude and results in a sharp decrease of Teff towards equilibrium.

  14. Predation by and activity patterns of ‘parasitic’ beetles of the genus Amblyopinus (Coleoptera: Staphylinidae)

    E-Print Network [OSTI]

    Ashe, James S.; Timm, Robert M.

    1987-07-01

    derivatives of their hosts. We examined the mode of attachment, behaviour, and feeding activities of 254 Amblyopinus (A. tiptoni and A. emarginatus) on 69 hosts which were captured in Sherman live traps. In addition, similar information and diurnal activity...

  15. Reasoning Exercises in Assisted Living: a cluster randomized trial to improve reasoning and everyday problem solving

    E-Print Network [OSTI]

    Williams, Kristine; Herman, Ruth; Bontempo, Daniel

    2014-06-25

    in Assisted living activity Ultraviolet index number Exposure level Time to burn, minutes Actions to take 0, 1, 2 Minimal 60 Apply sPF 15 sunscreen 3, 4 low 45 Apply sPF 15 sunscreen; wear a hat 5, 6 Moderate 30 Apply sPF 15 sunscreen; wear a hat 7, 8, 9... high 15–25 Apply sPF 15–30 sunscreen; wear a hat and sunglasses 10 or higher Very high 10 Apply sPF 30 sunscreen; wear a hat, sunglasses, and protective clothing The Us national Weather service issues daily forecasts of ultraviolet levels...

  16. DYNAMIC MODELLING OF LIVING ANIONIC SOLUTION POLYMERIZATION OF STYRENE/BUTADIENE/DIVINYLBENZENE

    E-Print Network [OSTI]

    Schittkowski, Klaus

    DYNAMIC MODELLING OF LIVING ANIONIC SOLUTION POLYMERIZATION OF STYRENE/BUTADIENE model for the living anionic solution polymerization of styrene/butadiene/divinylbenzene in a continuous kinetic reactor model for the living anionic solution polymerization of styrene/butadiene

  17. Long-Range Untethered Real-Time Live Gas Main Robotic Inspection System

    SciTech Connect (OSTI)

    Hagen Schempf; Daphne D'Zurko

    2004-10-31

    Under funding from the Department of Energy (DOE) and the Northeast Gas Association (NGA), Carnegie Mellon University (CMU) developed an untethered, wireless remote controlled inspection robot dubbed Explorer. The project entailed the design and prototyping of a wireless self-powered video-inspection robot capable of accessing live 6- and 8-inch diameter cast-iron and steel mains, while traversing turns and Ts and elbows under real-time control with live video feedback to an operator. The design is that of a segmented actively articulated and wheel-leg powered robot design, with fisheye imaging capability and self-powered battery storage and wireless real-time communication link. The prototype was functionally tested in an above ground pipe-network, in order to debug all mechanical, electrical and software subsystems, and develop the necessary deployment and retrieval, as well as obstacle-handling scripts. A pressurized natural gas test-section was used to certify it for operation in natural gas at up to 60 psig. Two subsequent live-main field-trials in both cast-iron and steel pipe, demonstrated its ability to be safely launched, operated and retrieved under real-world conditions. The system's ability to safely and repeatably exidrecover from angled and vertical launchers, traverse multi-thousand foot long pipe-sections, make T and varied-angle elbow-turns while wirelessly sending live video and handling command and control messages, was clearly demonstrated. Video-inspection was clearly shown to be a viable tool to understand the state of this critical buried infrastructure, irrespective of low- (cast-iron) or high-pressure (steel) conditions. This report covers the different aspects of specifications, requirements, design, prototyping, integration and testing and field-trialing of the Explorer platform.

  18. Thermal Imaging of Single Living Cells Using Semiconductor Quantum Dots

    E-Print Network [OSTI]

    Yang, Jui-Ming

    2009-01-01

    A novel method of thermal activation and temperatureS. Ishiwata, "Imaging of thermal activation of actomyosinbeen used to image thermal activation of single actomyosin

  19. The Marine Live Bait Trade in California: A Pathway for Introduction of Non-Indigenous Species?

    E-Print Network [OSTI]

    Passarelli, Bruno

    2010-01-01

    To investigate the transport of NIS into California via theCalifornia via the Marine Live Bait Trade TransportCalifornia via the Marine Live Bait Trade Transport

  20. Activated sludge process: Waste treatment. (Latest citations from the Biobusiness database). Published Search

    SciTech Connect (OSTI)

    Not Available

    1993-10-01

    The bibliography contains citations concerning the use of the activated sludge process in waste and wastewater treatment. Topics include biochemistry of the activated sludge process, effects of various pollutants on process activity, effects of environmental variables such as oxygen and water levels, and nutrient requirements of microorganisms employed in activated sludge processes. The citations also explore use of the process to treat specific wastes, such as halocarbons, metallic wastes, and petrochemical effluents; and wastes from pharmaceutical and dairy processes. (Contains 250 citations and includes a subject term index and title list.)

  1. Activated sludge process: Waste treatment. (Latest citations from the Biobusiness database). Published Search

    SciTech Connect (OSTI)

    Not Available

    1993-07-01

    The bibliography contains citations concerning the use of the activated sludge process in waste and wastewater treatment. Topics include biochemistry of the activated sludge process, effects of various pollutants on process activity, effects of environmental variables such as oxygen and water levels, and nutrient requirements of microorganisms employed in activated sludge processes. The citations also explore use of the process to treat specific wastes, such as halocarbons, metallic wastes, and petrochemical effluents; and wastes from pharmaceutical and dairy processes. (Contains 250 citations and includes a subject term index and title list.)

  2. Activated-sludge process: Waste treatment. (Latest citations from the biobusiness database). Published Search

    SciTech Connect (OSTI)

    Not Available

    1992-07-01

    The bibliography contains citations concerning the use of the activated sludge process in waste and wastewater treatment. Topics include biochemistry of the activated sludge process, effects of various pollutants on process activity, effects of environmental variables such as oxygen and water levels, and nutrient requirements of microorganisms employed in activated sludge processes. The citations also explore use of the process to treat specific wastes, such as halocarbons, metallic wastes, and petrochemical effluents; and wastes from pharmaceutical and dairy processes. (Contains 250 citations and includes a subject term index and title list.)

  3. The Administration of Intranasal Live Attenuated Influenza Vaccine Induces Changes in the Nasal Microbiota and Nasal Epithelium Gene Expression Profile

    E-Print Network [OSTI]

    Tarabichi, Yasir

    2015-01-01

    56 vi The Administration of Intranasal Live AttenuatedCALIFORNIA Los Angeles The Administration of Intranasal LiveABSTRACT OF THE THESIS The Administration of Intranasal Live

  4. Design for the Frail Old: Environmental and Perceptual Influences on Corridor Walking Behaviors of Assisted Living Residents 

    E-Print Network [OSTI]

    Lu, Zhipeng

    2010-10-12

    of the expanding business of assisted living and other long-term care alternatives (e.g., adult day care), nursing home use substantially declined during the past ten years, even though the number of frail older people considered as potential nursing home users... of empirical studies: Correlates of physical activity in older adults ....................................................................................... 21 2.3 A review of design guidelines/recommendations from design studies 40...

  5. Coexistence and efficiency of normal and anomalous transport by molecular motors in living cells

    E-Print Network [OSTI]

    Igor Goychuk; Vasyl O. Kharchenko; R. Metzler

    2013-09-26

    Recent experiments reveal both passive subdiffusion of various nanoparticles and anomalous active transport of such particles by molecular motors in the molecularly crowded environment of living biological cells. Passive and active microrheology reveals that the origin of this anomalous dynamics is due to the viscoelasticity of the intracellular fluid. How do molecular motors perform in such a highly viscous, dissipative environment? Can we explain the observed co-existence of the anomalous transport of relatively large particles of 100 to 500 nm in size by kinesin motors with the normal transport of smaller particles by the same molecular motors? What is the efficiency of molecular motors in the anomalous transport regime? Here we answer these seemingly conflicting questions and consistently explain experimental findings in a generalization of the well-known continuous diffusion model for molecular motors with two conformational states in which viscoelastic effects are included.

  6. Saving fourth generation and baryon number by living long

    E-Print Network [OSTI]

    Hitoshi Murayama; Vikram Rentala; Jing Shu; Tsutomu T. Yanagida

    2010-12-01

    Recent studies of precision electroweak observables have led to the conclusion that a fourth generation is highly constrained. However, we point out that a long-lived fourth generation can reopen a large portion of the parameter space. In addition, it preserves baryon and lepton asymmetries against sphaleron erasure even if $B-L=0$. It opens up the possibility of exact $B-L$ symmetry and hence Dirac neutrinos. The fourth generation can be observed at the LHC with unique signatures of long-lived particles in the near future.

  7. Living well, doing good | Y-12 National Security Complex

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformationJesse BergkampCenter (LMI-EFRC) ProximityCenterLeeincreases |Living aLiving

  8. The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living a core experience for First

    E-Print Network [OSTI]

    The Sustainability FYE Cluster The Sustainability FYE Cluster will make sustainable urban living University. The Sustainability community infuses the learning-living community with practical and theoretical approaches to sustainable living, merging students' living community with unique academic and field

  9. Aquat. Living Resour. 23, 267276 (2010) c EDP Sciences, IFREMER, IRD 2010

    E-Print Network [OSTI]

    Pierce, Graham

    2010-01-01

    www.alr-journal.org Aquatic Living Resources Mesoscale effects of aquaculture installations on benthic

  10. Final Report on MAPPR Project: The Detroit Living Wage Ordinance: Will it Reduce Urban Poverty?

    E-Print Network [OSTI]

    Riley, Shawn J.

    Final Report on MAPPR Project: The Detroit Living Wage Ordinance: Will it Reduce Urban Poverty? David Neumark May 30, 2001 #12;Detroit's Living Wage Ordinance The Detroit Living Wage Ordinance passed in the fall of 1998. As in other major urban areas passing living wage ordinances in the 1990s, Detroit

  11. October 11, 2011 Keeping In Touch . . . Impacting Lives

    E-Print Network [OSTI]

    October 11, 2011 Keeping In Touch . . . Impacting Lives SCHOOL OF ALLIED HEALTH PROFESSIONS LSUHSC · School of Allied Health Professions · 1900 Gravier Street · New Orleans, LA 70112 · 504-568-4246 · www.alliedhealth.lsuhsc.edu May 19, 2014 Growing our Health CareTeam The School of Allied Health Professions (SAHP

  12. Framing Change: Social Movement Framing in University Living Wage Movements 

    E-Print Network [OSTI]

    Metcalf, Laurie D.

    2010-01-14

    of these living wage movements, two cases, Texas A&M University and Georgetown University, were selected for this study to examine through the lens of the social movement framing perspective. Data for the cases included interviews with activists and administrators...

  13. Flow-induced gelation of living (micellar) polymers Robijn Bruinsma

    E-Print Network [OSTI]

    Ben-Shaul, Avinoam

    Flow-induced gelation of living (micellar) polymers Robijn Bruinsma Department of Physics-deformationeffectsdue to flow. Steady-statesolutions to the kinetic equationareobtained,with the correspondingmean micellar size (z) evaluatedasa function of the PecletnumberP, i.e., the dimensionlessratio of flow rate i

  14. Distribution of ranks of ?-decay half-lives

    E-Print Network [OSTI]

    Juan Miguel Campanario

    2010-11-21

    I studied the distribution of ranks of values of 2949 {\\beta}-decay half-lives according to an empirical beta law with two exponents. {\\beta}-decay half-life ranks showed good fit to a beta function with two exponents.

  15. Enhancing the Performance of High Availability Lightweight Live Migration

    E-Print Network [OSTI]

    Ravindran, Binoy

    on the primary host, and transfers the latest checkpoint to the backup host as whole-system migration. Once is that the primary host migrates the guest VM image (including CPU/memory status updates and new writes to the fileEnhancing the Performance of High Availability Lightweight Live Migration Peng Lu1, Binoy Ravindran

  16. Continental ood basalts: episodic magmatism above long-lived hotspots

    E-Print Network [OSTI]

    Johnston, Stephen T.

    November 1999 Abstract The eruption of continental flood basalt (CFB) may reflect episodic magmatism above long-lived mantle plumes. The Iceland and Yellowstone hotspots have generated successive CFB provinces in subducting oceanic lithosphere led to subsequent breakthrough and eruption of CFB. Since both mantle plume

  17. ith America focused on energy alternatives and green living,

    E-Print Network [OSTI]

    Veiga, Pedro Manuel Barbosa

    W ith America focused on energy alternatives and green living, Oklahoma State University- Tulsa. His process to produce a clean, alternative energy source could significantly reduce the use of fossil it a very attractive alternative to solar energy technology. "Thermoelectric materials are so much less

  18. Working Together to Save Lives National Weather Service Strategic Plan

    E-Print Network [OSTI]

    Working Together to Save Lives National Weather Service Strategic Plan for 2005-2010 #12;i Preface The National Oceanic and Atmospheric Administration (NOAA) Strategic Plan (http for a broader range of environmental information services. NOAA's National Weather Service (NWS) Strategic Plan

  19. Saving Lives, Time and Resources tti.tamu.edu

    E-Print Network [OSTI]

    . TTI research is widely known as an excellent value with a proven impact of saving lives, time grounds facility, environmental and emissions facility and sediment and erosion control laboratory eight offices in Texas and offices in Ann Arbor, MI; Washington, D.C.; and Mexico City. TTI also has

  20. Computational adaptive optics for live three-dimensional biological imaging

    E-Print Network [OSTI]

    Agard, David

    Computational adaptive optics for live three- dimensional biological imaging Z. Kam*, B. Hanser , M. Under their design conditions, modern microscope optics produce nearly ideal aberration-free imaging to the coverslip. When focusing into thick samples, the 3D optical characteristics of the sample itself must

  1. Shrinky Dink microbes! icrobes are living organisms smaller

    E-Print Network [OSTI]

    Lawrence, Rick L.

    Shrinky Dink microbes! M icrobes are living organisms smaller than your eyes can see in Yellowstone National Park. Scientists at Montana State University (MSU) are researching how microbes from. Some kinds of microbes, called extremophiles, thrive in places that are freezing, super hot, deep

  2. Safety and Liveness in Intelligent Intersections Hemant Kowshik1

    E-Print Network [OSTI]

    of designing intelligent intersections where traffic lights and stop signs are removed, and cars negotiate intersections are representative of complex distributed hybrid systems which need architectures and algorithms with provable safety and liveness. We propose a hybrid architecture which involves an appropriate inter- play

  3. New York City Visiting or living in New York

    E-Print Network [OSTI]

    Navigating New York City #12;·Visiting or living in New York for the first time is an interesting. #12;Learn the geography of New York City's five boroughs. 1) Manhattan 2) Brooklyn 3) Queens 4) Bronx Major airports serving New York City: 1) John F. Kennedy International Airport (JFK) 2) La

  4. Motivating Mobility: Designing for Lived Motivation in Stroke Rehabilitation

    E-Print Network [OSTI]

    Balaam, Madeline

    Motivating Mobility: Designing for Lived Motivation in Stroke Rehabilitation Madeline Balaam1 rehabilitation exercise after stroke. We report on participatory design work with four stroke survivors to develop a holistic understanding of their motivation and rehabilitation needs, and to construct and deploy

  5. Have You Visited the Living Zero Home Tour?

    Broader source: Energy.gov [DOE]

    The Living Zero Home Tour is on the road, and it may be in a city near you! If you have the chance to see the tour, you may be surprised to discover the small steps that you can take to save energy...

  6. Road to Discovery Blog Riding for the Lives of Children

    E-Print Network [OSTI]

    Buechler, Steven

    #12;BOSTON 6.13.11 DALLAS 7.8.11 Road to Discovery Blog Riding for the Lives of Children 2 for us. " " #12;day 1W e had the perfect day today! The weather was a bit chilly at first departed from Ara Parseghian's home in the Boston area. Ara is the grandson of Coach Ara Parseghian, one

  7. Metal-induced energy transfer for live cell nanoscopy

    E-Print Network [OSTI]

    Enderlein, Jörg

    Metal-induced energy transfer for live cell nanoscopy Alexey I. Chizhik1 *, Jan Rother2 , Ingo transfer from an optically excited donor to an acceptor. We replace the acceptor molecule with a metallic film and use the measured energy transfer efficiency from donor molecules to metal surface plasmons2

  8. Health Foods, Healthy Lives Institute Grants Awarded March 2011

    E-Print Network [OSTI]

    Amin, S. Massoud

    1 Health Foods, Healthy Lives Institute Grants Awarded March 2011 Food Safety "Prevalence of Medicine) Co-Investigators: Jeff Bender, DVM, Veterinary Public Health (College of Veterinary Medicine and Intl Medicine (School of Medicine) David Boxrud, MS, Molecular Typing Laboratory (MN Dept of Health

  9. Brazil's Right to Save Lives (NYT) 484 words

    E-Print Network [OSTI]

    Lopez-Carr, David

    Brazil's Right to Save Lives (NYT) 484 words Published: June 23, 2005 Brazil has the best anti-name drugs. Brazil can freely copy any drug commercialized before 1997, when the country began to respect are still imported and are expensive, and Brazil is spending two-thirds of its antiretroviral budget on just

  10. Living our dying: Dewey, death and aesthetic sensibility 

    E-Print Network [OSTI]

    Zgarba, Rex Jason

    1997-01-01

    the Internet on NPR's web site at www. npr. org. game called "Creatures. " In the course of the game the player receives six eggs, an incubator, and a whole virtual world in which the creatures can 'live'. They move, eat, grow, get sick, sleep and reproduce...

  11. Living in a Smart Environment Implications for the Coming Ubiquitous

    E-Print Network [OSTI]

    - ciplinary research project "Living in a Smart Environ- ment ­ Implications of Ubiquitous Computing". To show from a large-scale de- ployment of ubiquitous and pervasive computing tech- nologies. Second, it investigates issues of social compati- bility and dependability of future ubiquitous computing applications

  12. Public Health in Public Housing: Improving Health, Changing Lives

    E-Print Network [OSTI]

    Shen, Jun

    Public Health in Public Housing: Improving Health, Changing Lives National Heart, Lung, and Blood Institute Education Strategy Development Workshop S U M M A R Y R E P O R T U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of Health National Heart, Lung, and Blood Institute #12;#12;U

  13. How can we build enough physical activity into our busy lives, and

    E-Print Network [OSTI]

    Neimark, Alexander V.

    Child's TV Time 5 Life Expectancy: Health and Wealth Connections 6 Recent Salmonella Outbreak in Peanut, avoid gaming and porn websites. 3- Put a lock on yo

  14. Global and regional evolution of short-lived radiatively-active...

    Office of Scientific and Technical Information (OSTI)

    Authors: Lamarque, J.-F. ; Kyle, G. Page ; Meinshausen, Malte ; Riahi, Keywan ; Smith, Steven J. ; Van Vuuren, Detlef ; Conley, Andrew ; Vitt, Francis Publication Date:...

  15. Fossil identification activity Standard: Comparing fossils to each other or to living organisms reveals features of

    E-Print Network [OSTI]

    . Icaronycteris XI6a, aka" bat" 4. Boney Fish (Knightera) XI2c, (1 piece) 5. Megalodon tooth XIa 6. Dinosaur skin? (teeth? skin? bone? imprint?) 3. What kind of animal is it? (reptile? mammal? invertebrate? fish?) 4. Why

  16. Global and regional evolution of short-lived radiatively-active gases and

    Office of Scientific and Technical Information (OSTI)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfate Reducing(Journal Article) |productionPatent:CompressionSciTechwithinProduced atGenes

  17. Uncharted Microbial World: Microbes and Their Activities in the Environment

    SciTech Connect (OSTI)

    Harwood, Caroline; Buckley, Merry.

    2007-12-31

    Microbes are the foundation for all of life. From the air we breathe to the soil we rely on for farming to the water we drink, everything humans need to survive is intimately coupled with the activities of microbes. Major advances have been made in the understanding of disease and the use of microorganisms in the industrial production of drugs, food products and wastewater treatment. However, our understanding of many complicated microbial environments (the gut and teeth), soil fertility, and biogeochemical cycles of the elements is lagging behind due to their enormous complexity. Inadequate technology and limited resources have stymied many lines of investigation. Today, most environmental microorganisms have yet to be isolated and identified, let alone rigorously studied. The American Academy of Microbiology convened a colloquium in Seattle, Washington, in February 2007, to deliberate the way forward in the study of microorganisms and microbial activities in the environment. Researchers in microbiology, marine science, pathobiology, evolutionary biology, medicine, engineering, and other fields discussed ways to build on and extend recent successes in microbiology. The participants made specific recommendations for targeting future research, improving methodologies and techniques, and enhancing training and collaboration in the field. Microbiology has made a great deal of progress in the past 100 years, and the useful applications for these new discoveries are numerous. Microorganisms and microbial products are now used in industrial capacities ranging from bioremediation of toxic chemicals to probiotic therapies for humans and livestock. On the medical front, studies of microbial communities have revealed, among other things, new ways for controlling human pathogens. The immediate future for research in this field is extremely promising. In order to optimize the effectiveness of community research efforts in the future, scientists should include manageable systems with features like clear physical boundaries, limited microbial diversity, and manipulability with the goal of understanding fundamental principles that may apply to more complex systems. A great deal of microbial genetic and phenotypic diversity remains to be explored, and the commercial and medical potential locked up in these unknowns should compel the field to move forward. Future microbiology research will build on the successes of the past using new techniques and approaches. Uncultivated microbes hold great promise for industry, medicine, and the recycling of precious resources, and research and technology must make inroads in overcoming the barriers that prevent their study. In many cases, we will no longer be able to rely on isolated, pure cultures of microorganisms, but must use communities of microorganisms, which presently are poorly understood. Indeed, community-level studies can benefit from deconstructing microbial communities and analyzing the component members separately, but this is not feasible in every system. The effects of perturbation on microbial communities also require study. Humans rely on the services of microbes in innumerable ways, but we have little or no predictive understanding of how microbial communities respond to disturbance. Research must address current limitations in detecting microscale interactions among microbes by enhancing current technologies and fostering new microscopic tools, biosensors, and gas sensors for appropriate small scales. Genomics, which has enabled great progress in microbiology research of individual species, must be applied to communities of microorganisms. This will require improved methods of DNA extraction and amplification from environmental samples and improved strategies for DNA sequence assembly. In the future, genome sequencing efforts should continue the exploration of evolutionarily diverse microbes, as well as help reveal the mechanisms by which closely related microbes evolve. Technological advances have spurred every great leap in microbial biology, and in order to move forward, new m

  18. Activation detector

    DOE Patents [OSTI]

    Bell, Zane William (Oak Ridge, TN) [Oak Ridge, TN; Boatner, Lynn Allen (Oak Ridge, TN) [Oak Ridge, TN

    2009-12-08

    A method of detecting an activator, the method including impinging with an activator a receptor material lacking a photoluminescent material and generating a by-product of a radioactive decay due to the activator impinging the reeptor material. The method further including, generating light from the by-product via the Cherenkov effect and identifying a characteristic of the activator based on the light.

  19. Aquat. Living Resour. (2007) c EDP Sciences, IFREMER, IRD 2008

    E-Print Network [OSTI]

    Sorin, Eric J.

    2007-01-01

    , and fishes to look at aspects of swimming perfor- mance, spawning behavior, foraging activity, and bioenerget

  20. Statistical Global Model of beta- Half-lives and r-Process Nucleosynthesis

    E-Print Network [OSTI]

    N. J. Costiris; E. Mavrommatis; K. A. Gernoth; J. W. Clark

    2013-09-02

    Purpose: Our objective is to apply an improved statistical global model of beta^- decay half-life systematics [1] generated by machine-learning techniques to the prediction of beta half-lives relevant to r-process nuclei. The primary aim of this application is to complement existing r-process-clock and matter-flow studies, thereby providing additional theoretical support for the planning of future activities of the world's network of rare-isotope laboratories. Results: Results are presented for nuclides situated on the r-ladders at N=50, 82, and 126 where abundances peak, as well as for nuclides that affect abundances between peaks or may be relevant to r-processes under different astrophysical scenarios. The half-lives of some of the targeted neutron-rich nuclides have either been recently measured or will be accessible at rare-isotope laboratories in the relatively near future. The results of our large-scale data-driven half-life calculations (generated by a "theory-thin" global statistical model) are compared to available experimental data, including recent measurements on very neutron-rich nuclei along an r-process path far from the valley of $\\beta$ stability. Comparison is also made with corresponding results from traditional global models derived by semi-phenomenological "theory-thick" approaches.

  1. KINEMATICS, STATICS, AND DEXTERITY OF PLANAR ACTIVE STRUCTURE MODULES

    E-Print Network [OSTI]

    Williams II, Robert L.

    trusses). Unfortunately, the few systems actually built to date have not delivered these desired waste remediation [4,5]. Many authors have presented results for active structures, but the work has not lived up to the promise of active structures because they are too heavy, too flexible, too slow

  2. Single-photon ultrashort-lived radionuclides: symposium proceedings

    SciTech Connect (OSTI)

    Paras, P.; Thiessen, J.W. (eds.)

    1985-01-01

    The purpose was to define the current role and state-of-the-art regarding the development, clinical applications, and usefulness of generator-produced single-photon ultrashort-lived radionuclides (SPUSLR's) and to predict their future impact on medicine. Special emphasis was placed on the generator production of iridium-191, gold-195, and krypton-81. This report contains expanded summaries of the included papers. (ACR)

  3. ClimateChangeLIVE Webcast: Join the Climate Conversation

    Broader source: Energy.gov [DOE]

    Join ClimateChangeLIVE's webcast, bringing together students and climate experts for a discussion about climate change and what students and classes around the country are doing to be part of the climate solution. Students will be able to interact with climate scientists and experts online through Facebook and Twitter. A GreenWorks! grant will be offered to help schools with climate action projects.

  4. The problem of living in a world contaminated with chemicals

    SciTech Connect (OSTI)

    Metcalf, R.L.

    1990-12-31

    The proliferation of xenobiotic chemicals in the global environment poses living problems for each of us aboard {open_quotes}spaceship earth.{close_quotes} Seven case studies are presented that illustrate the magnitude of the problem that can result from waiting to identify toxic hazards until there have been decades of {open_quotes}human guinea pig{close_quotes} exposure. 25 refs., 5 tabs.

  5. Half-lives of Double $?^+$-decay with Two Neutrinos

    E-Print Network [OSTI]

    Yuejiao Ren; Zhongzhou Ren

    2015-01-07

    Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated half-lives of double $\\beta ^+$-decay in this article will be useful for future experimental search of double $\\beta ^+$-decay.

  6. Identification of novel antimicrobials using a live-animal infection model

    E-Print Network [OSTI]

    Ausubel, Frederick M.

    pathogens points to the need for novel therapeutic approaches to combat infection. To discover novel nematode Caenorhabditis elegans infected with the human opportunistic pathogen Enterococcus faecalis. E the overmining of culti- vable microorganisms (11), a high background of toxic compounds or compounds with poor

  7. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir; Sanjiv (Portola Valley, CA), Pritha; Ray (Mountain View, CA)

    2009-04-28

    Novel double and triple fusion reporter gene constructs harboring distinct imageable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  8. TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing...

    Office of Environmental Management (EM)

    ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's Oil Dependence Through Innovation TODAY: ARPA-E Director Arun Majumdar to Host Live Chat on Reducing America's...

  9. Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals

    DOE Patents [OSTI]

    Gambhir, Sanjiv (Portola Valley, CA); Pritha, Ray (Mountain View, CA)

    2011-06-07

    Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.

  10. Living, Eating and Learning: Children’s Experiences of Change and Life in a Refugee Camp 

    E-Print Network [OSTI]

    Atkinson, Lucy C

    2007-01-01

    This is a study about children living in an unusual setting, a refugee camp. It recognizes that this situation causes disruption to children’s lives but rather than focusing exclusively on this disruption, emphasizes the ...

  11. Live wires: direct extracellular electron exchange for bioenergy and the bioremediation of energy-related contamination

    SciTech Connect (OSTI)

    Lovley, DR

    2011-12-01

    Microorganisms that can form direct electrical connections with insoluble minerals, electrodes, or other microorganisms can play an important role in some traditional as well as novel bioenergy strategies and can be helpful in the remediation of environmental contamination resulting from the use of more traditional energy sources. The surprising discovery that microorganisms in the genus Geobacter are capable of forming highly conductive networks of filaments that transfer electrons along their length with organic metallic-like conductivity, rather than traditional molecule to molecule electron exchange, provides an explanation for the ability of Geobacter species to grow in subsurface environments with insoluble Fe(III) oxides as the electron acceptor, and effectively remediate groundwater contaminated with hydrocarbon fuels or uranium and similar contaminants associated with the mining and processing of nuclear fuel. A similar organic metallic-like conductivity may be an important mechanism for microorganisms to exchange electrons in syntrophic associations, such as those responsible for the conversion of organic wastes to methane in anaerobic digesters, a proven bioenergy technology. Biofilms with conductivities rivaling those of synthetic polymers help Geobacter species generate the high current densities in microbial fuel cells producing electric current from organic compounds. Electron transfer in the reverse direction, i.e. from electrodes to microbes, is the basis for microbial electrosynthesis, in which microorganisms reduce carbon dioxide to fuels and other useful organic compounds with solar energy in a form of artificial photosynthesis that is more efficient and avoids many of the environmental sustainability concerns associated with biomass-based bioenergy strategies. The ability of Geobacter species to produce highly conductive electronic networks that function in water opens new possibilities in the emerging field of bioelectronics.

  12. Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy

    E-Print Network [OSTI]

    Huang, Yanyi

    Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy non-fluorescent nanodiamonds in living cells using transient absorption microscopy. This label of nanodiamonds under various conditions, confirming the endocytosis mechanism. Optical probes for live cell

  13. Aalborg Universitet Intelligent DC Microgrid Living Laboratories -A Chinese-Danish Project

    E-Print Network [OSTI]

    Vasquez, Juan Carlos

    Aalborg Universitet Intelligent DC Microgrid Living Laboratories - A Chinese-Danish Project Diaz Microgrid Living Laboratories - A Chinese-Danish Project. In IEEE ICDCM 2015. IEEE Press. General rights.aau.dk on: juli 07, 2015 #12;Intelligent DC Microgrid Living Laboratories - A Chinese-Danish Project Enrique

  14. Live Migration of Direct-Access Asim Kadav and Michael M. Swift

    E-Print Network [OSTI]

    Swift, Michael

    12/17/2008 1 Live Migration of Direct-Access Devices Asim Kadav and Michael M. Swift University of Wisconsin - Madison Live Migration · Migrating VM across different hosts without noticeable downtime · Uses of Live Migration ­ Reducing energy consumption by hardware consolidation ­ Perform non

  15. Mitigating container security risk using real-time monitoring with active Radio Frequency Identification and sensors

    E-Print Network [OSTI]

    Schlesinger, Adam Ian

    2005-01-01

    The global village in which we live enables increased trade and commerce across regions but also brings a complicated new set of challenges such as terrorist activity, human and drug smuggling and theft in foreign or ...

  16. Living Apart Together and Cohabitation Intentions in Great Britain

    E-Print Network [OSTI]

    Coulter, Rory; Hu, Yang

    2015-07-15

    where the reference category is LAT. The pseudo-r2 and reduction in log-likelihood indicate that the model fits well. As anticipated, cohort is a strong predictor of partnership status. Older cohorts are more likely than those aged 16-24 to be married... , S., & Stoilova, M. (2014). Practices and perceptions of living apart together. Family Science, 5, 1–10. Elder, G. H., Johnson, M. K., & Crosnoe, R. (2003). The emergence and development of life course theory. In J. T. Mortimer & M. J. Shanahan...

  17. MHK Projects/Live Oak Project | Open Energy Information

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIXsource HistoryScenarios Towards 2050 JumpCoos Bay OPTHalf MoonKillisnooLeanconLive Oak

  18. Long-Living Polymer Electrolytes | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious RankADVANCED MANUFACTURING OFFICE INDUSTRIALU.S.Leadership on CleanUpList ofIncreaseLong-Living Polymer

  19. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power AdministrationRobust,Field-effectWorking With U.S.Week DayDr.Theories81TowardsTracking Living Cells as They

  20. Stent Technology Saves Lives, Creates Jobs | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX E LIST OF APPLICABLEStatutory Authority for an SPR DrawdownStent Technology Saves Lives,

  1. Live from the Clinton Global Initiative | Department of Energy

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on DeliciousMathematicsEnergyInterested Parties - WAPA PublicLED ADOPTIONtoLightingEnergyEnergyLive from the

  2. How Will We Live Forever? | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity ofkandz-cm11 Outreach Home Room NewsInformation CurrentHenry Bellamy, Ph.D.Food Drive HolidayHours UsedFireAmberMindLive

  3. Firearm equipped with live round inhibiting means and method of making same

    DOE Patents [OSTI]

    Baehr, D.G.

    1990-11-13

    A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means. 9 figs.

  4. Firearm equipped with live round inhibiting means and method of making same

    DOE Patents [OSTI]

    Baehr, Donald G. (Albuquerque, NM)

    1990-01-01

    A firearm is disclosed having live round inhibiting means mounted in the barrel of the firearm which permits a blank cartridge to be loaded into a firearm and fired while preventing the loading and firing of a live round. The live round inhibiting means comprise shaft means mounted in the barrel of the firearm and which extends a sufficient length into the barrel at a point just beyond the chamber portion of the firearm to engage the bullet portion of a live round to prevent it from properly chambering, while permitting a blank cartridge to be loaded into the firearm and fired without engaging the live round-inhibiting shaft means.

  5. Experimental test of whether electrostatically charged micro-organisms and their spores contribute to the onset of arcs across vacuum gaps

    SciTech Connect (OSTI)

    Grisham, L. R.; Halle, A. von; Carpe, A. F.; Gilton, K. R.; Rossi, Guy; Stevenson, T. N.

    2013-12-15

    Recently it was proposed [L. R. Grisham et al. Phys. Plasmas 19, 023107 (2012)] that one of the initiators of vacuum voltage breakdown between conducting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which then become electrostatically charged when an electric potential is applied across the vacuum gap. This note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maximum operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each case preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance.

  6. Experimental Test Of Whether Electrostatically Charged Micro-organisms And Their Spores Contribute To The Onset Of Arcs Across Vacuum Gaps

    SciTech Connect (OSTI)

    none,; Grisham, Larry R.

    2014-02-24

    Recently it was proposed [L.R. Grisham, A. vonHalle, A.F. Carpe, Guy Rossi, K.R. Gilton, E.D. McBride, E.P. Gilson, A. Stepanov, T.N. Stevenson, Physics of Plasma 19 023107 (2012)] that one of the initiators of vacuum voltage breakdown between condu cting electrodes might be micro-organisms and their spores, previously deposited during exposure to air, which tnen become electrostatically charged when an electric potential is applied across the vacuum gap. The note describes a simple experiment to compare the number of voltage-conditioning pulses required to reach the nominal maxium operating voltage across a gap between two metallic conductors in a vacuum, comparing cases in which biological cleaning was done just prior to pump-down with cases where this was not done, with each preceded by exposure to ambient air for three days. Based upon these results, it does not appear that air-deposited microbes and their spores constitute a major pathway for arc initiation, at least for exposure periods of a few days, and for vacuum gaps of a few millimeters, in the regime where voltage holding is usually observed to vary linearly with gap distance

  7. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, Armin

    2014-09-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed.

  8. "Each and everyone of us can make changes in the way we live our lives and become part of the solution [to climate change]"

    E-Print Network [OSTI]

    Scharfstein, Daniel

    Green Guide "Id put my money on the sun and solar energy. What a source of power! I hope we dont have of the solution [to climate change]" Al Gore, ,,An Inconvenient Truth BaltimoreGreen Living Guide www.jhsph.edu/green content useful. Cheers, and happy green living! "Be the change you want to see in the world" -- Gandhi

  9. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    regulates protein function in a cell, either activating or inactivating the proteins responsible for many cell functions ranging from cell proliferation to...

  10. Tracking Living Cells as They Differentiate in Real Time

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    absorption spectra. Phosphorylating enzymes add one or more phosphate groups to three amino-acid residues common in proteins - serine, threonine, or tyrosine - which activates...

  11. Short-lived lattice quasiparticles for strongly interacting fluids

    E-Print Network [OSTI]

    Mendoza, M

    2015-01-01

    It is shown that lattice kinetic theory based on short-lived quasiparticles proves very effective in simulating the complex dynamics of strongly interacting fluids (SIF). In particular, it is pointed out that the shear viscosity of lattice fluids is the sum of two contributions, one due to the usual interactions between particles (collision viscosity) and the other due to the interaction with the discrete lattice (propagation viscosity). Since the latter is {\\it negative}, the sum may turn out to be orders of magnitude smaller than each of the two contributions separately, thus providing a mechanism to access SIF regimes at ordinary values of the collisional viscosity. This concept, as applied to quantum superfluids in one-dimensional optical lattices, is shown to reproduce shear viscosities consistent with the AdS-CFT holographic bound on the viscosity/entropy ratio. This shows that lattice kinetic theory continues to hold for strongly coupled hydrodynamic regimes where continuum kinetic theory may no longer...

  12. Short-lived isomers in {sup 94}Rb

    SciTech Connect (OSTI)

    Tsekhanovich, I.; Dare, J. A.; Smith, A. G.; Varley, B. J.; Simpson, G. S.; Urban, W.; Soldner, T.; Jolie, J.; Linnemann, A.; Orlandi, R.; Smith, J. F.; Scherillo, A.; Rzaca-Urban, T.; Zlomaniec, A.; Dorvaux, O.; Gall, B. J. P.; Roux, B.

    2008-07-15

    The medium-spin structure of the neutron-rich, odd-odd nucleus {sup 94}Rb was studied by means of {gamma}-ray spectroscopy. Excited levels were populated in the neutron-induced fission of {sup 235}U and in the spontaneous fission of {sup 252}Cf and {sup 248}Cm. Two isomeric states were found at 1485.2 and 2074.8 keV with half-lives of 18 and 107 ns, respectively. The probable structures of the two isomers involve the fully aligned, proton-neutron configurations [{pi}(g{sub 9/2}) x {nu}(g{sub 7/2})]{sub 8{sup +}} and [{pi}(g{sub 9/2}) x {nu}(h{sub 11/2})]{sub 10{sup -}}, respectively. These new data give information on the single-particle energies in the region.

  13. Diffusion Controlled Reactions, Fluctuation Dominated Kinetics, and Living Cell Biochemistry

    E-Print Network [OSTI]

    Konkoli, Zoran

    2009-01-01

    In recent years considerable portion of the computer science community has focused its attention on understanding living cell biochemistry and efforts to understand such complication reaction environment have spread over wide front, ranging from systems biology approaches, through network analysis (motif identification) towards developing language and simulators for low level biochemical processes. Apart from simulation work, much of the efforts are directed to using mean field equations (equivalent to the equations of classical chemical kinetics) to address various problems (stability, robustness, sensitivity analysis, etc.). Rarely is the use of mean field equations questioned. This review will provide a brief overview of the situations when mean field equations fail and should not be used. These equations can be derived from the theory of diffusion controlled reactions, and emerge when assumption of perfect mixing is used.

  14. Magnetic resonance imaging of living systems by remote detection

    DOE Patents [OSTI]

    Wemmer, David; Pines, Alexander; Bouchard, Louis; Xu, Shoujun; Harel, Elad; Budker, Dmitry; Lowery, Thomas; Ledbetter, Micah

    2013-10-29

    A novel approach to magnetic resonance imaging is disclosed. Blood flowing through a living system is prepolarized, and then encoded. The polarization can be achieved using permanent or superconducting magnets. The polarization may be carried out upstream of the region to be encoded or at the place of encoding. In the case of an MRI of a brain, polarization of flowing blood can be effected by placing a magnet over a section of the body such as the heart upstream of the head. Alternatively, polarization and encoding can be effected at the same location. Detection occurs at a remote location, using a separate detection device such as an optical atomic magnetometer, or an inductive Faraday coil. The detector may be placed on the surface of the skin next to a blood vessel such as a jugular vein carrying blood away from the encoded region.

  15. Subdiffraction-limited quantum imaging within a living cell

    E-Print Network [OSTI]

    Michael A. Taylor; Jiri Janousek; Vincent Daria; Joachim Knittel; Boris Hage; Hans-A. Bachor; Warwick P. Bowen

    2014-02-05

    We report both sub-diffraction-limited quantum metrology and quantum enhanced spatial resolution for the first time in a biological context. Nanoparticles are tracked with quantum correlated light as they diffuse through an extended region of a living cell in a quantum enhanced photonic force microscope. This allows spatial structure within the cell to be mapped at length scales down to 10 nm. Control experiments in water show a 14% resolution enhancement compared to experiments with coherent light. Our results confirm the longstanding prediction that quantum correlated light can enhance spatial resolution at the nanoscale and in biology. Combined with state-of-the-art quantum light sources, this technique provides a path towards an order of magnitude improvement in resolution over similar classical imaging techniques.

  16. Near-Term Climate Mitigation by Short-Lived Forcers

    SciTech Connect (OSTI)

    Smith, Steven J.; Mizrahi, Andrew H.

    2013-08-12

    Emissions reductions focused on anthropogenic climate forcing agents with relatively short atmospheric lifetimes such as methane (CH4) and black carbon (BC) have been suggested as a strategy to reduce the rate of climate change over the next several decades. We find that reductions of methane and BC would likely have only a modest impact on near-term climate warming. Even with maximally feasible reductions phased in from 2015 to 2035, global mean temperatures in 2050 are reduced by 0.16 °C, with an uncertainty range of 0.04-0.36°C, with the high end of this range only possible if total historical aerosol forcing is small. More realistic mitigation scenarios would likely provide a smaller climate benefit. The climate benefits from targeted reductions in short-lived forcing agents are smaller than previously estimated and are not substantially different in magnitude from the benefits due to a comprehensive climate policy.

  17. Signal focusing through active transport

    E-Print Network [OSTI]

    Aljaz Godec; Ralf Metzler

    2015-01-13

    In biological cells and novel diagnostic devices biochemical receptors need to be sensitive to extremely small concentration changes of signaling molecules. The accuracy of such molecular signaling is ultimately limited by the counting noise imposed by the thermal diffusion of molecules. Many macromolecules and organelles transiently bind to molecular motors and are then actively transported. We here show that a random albeit directed delivery of signaling molecules to within a typical diffusion distance to the receptor reduces the correlation time of the counting noise, effecting an improved sensing precision. The conditions for this active focusing are indeed compatible with observations in living cells. Our results are relevant for a better understanding of molecular cellular signaling and the design of novel diagnostic devices.

  18. Air Leakage and Air Transfer Between Garage and Living Space

    SciTech Connect (OSTI)

    Rudd, A.

    2014-09-01

    This research project focused on evaluation of air transfer between the garage and living space in a single-family detached home constructed by a production homebuilder in compliance with the 2009 International Residential Code and the 2009 International Energy Conservation Code. The project gathered important information about the performance of whole-building ventilation systems and garage ventilation systems as they relate to minimizing flow of contaminated air from garage to living space. A series of 25 multi-point fan pressurization tests and additional zone pressure diagnostic testing characterized the garage and house air leakage, the garage-to-house air leakage, and garage and house pressure relationships to each other and to outdoors using automated fan pressurization and pressure monitoring techniques. While the relative characteristics of this house may not represent the entire population of new construction configurations and air tightness levels (house and garage) throughout the country, the technical approach was conservative and should reasonably extend the usefulness of the results to a large spectrum of house configurations from this set of parametric tests in this one house. Based on the results of this testing, the two-step garage-to-house air leakage test protocol described above is recommended where whole-house exhaust ventilation is employed. For houses employing whole-house supply ventilation (positive pressure) or balanced ventilation (same pressure effect as the Baseline condition), adherence to the EPA Indoor airPLUS house-to-garage air sealing requirements should be sufficient to expect little to no garage-to-house air transfer.

  19. Towards a 'Thermodynamics' of Active Matter

    E-Print Network [OSTI]

    Sho C. Takatori; John F. Brady

    2014-11-21

    Self-propulsion allows living systems to display unusual collective behavior. Unlike passive systems in thermal equilibrium, active matter systems are not constrained by conventional thermodynamic laws. A question arises however as to what extent, if any, can concepts from classical thermodynamics be applied to nonequilibrium systems like active matter. Here we use the new swim pressure perspective to develop a simple theory for predicting phase separation in active matter. Using purely mechanical arguments we generate a phase diagram with a spinodal and critical point, and define a nonequilibrium chemical potential to interpret the "binodal." We provide a generalization of thermodynamic concepts like the free energy and temperature for nonequilibrium active systems. Our theory agrees with existing simulation data both qualitatively and quantitatively and may provide a framework for understanding and predicting the behavior of nonequilibrium active systems.

  20. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H.C.

    1997-12-30

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques. 5 figs.

  1. Cellulase producing microorganism ATCC 55702

    DOE Patents [OSTI]

    Dees, H. Craig (Lenoir City, TN)

    1997-01-01

    Bacteria which produce large amounts of cellulase--containing cell-free fermentate have been identified. The original bacterium (ATCC 55703) was genetically altered using nitrosoguanidine (MNNG) treatment to produce the enhanced cellulase producing bacterium (ATCC 55702), which was identified through replicate plating. ATCC 55702 has improved characteristics and qualifies for the degradation of cellulosic waste materials for fuel production, food processing, textile processing, and other industrial applications. ATCC 55702 is an improved bacterial host for genetic manipulations using recombinant DNA techniques, and is less likely to destroy genetic manipulations using standard mutagenesis techniques.

  2. Active Knowledge

    E-Print Network [OSTI]

    Freuder, Eugene C.

    A progress report on the work described in Vision Flashes 33 and 43 on recognition of real objects. Emphasis is on the "active" use of knowledge in directing the flow of visual processing.

  3. Nonequilibrium equation of state in suspensions of active colloids

    E-Print Network [OSTI]

    Félix Ginot; Isaac Theurkauff; Demian Levis; Christophe Ybert; Lydéric Bocquet; Ludovic Berthier; Cécile Cottin-Bizonne

    2014-11-26

    Active colloids constitute a novel class of materials composed of colloidal-scale particles locally converting chemical energy into motility, mimicking micro-organisms. Evolving far from equilibrium, these systems display structural organizations and dynamical properties distinct from thermalized colloidal assemblies. Harvesting the potential of this new class of systems requires the development of a conceptual framework to describe these intrinsically nonequilibrium systems. We use sedimentation experiments to probe the nonequilibrium equation of state of a bidimensional assembly of active Janus microspheres, and conduct computer simulations of a model of self-propelled hard disks. Self-propulsion profoundly affects the equation of state, but these changes can be rationalized using equilibrium concepts. We show that active colloids behave, in the dilute limit, as an ideal gas with an activity-dependent effective temperature. At finite density, increasing the activity is similar to increasing adhesion between equilibrium particles. We quantify this effective adhesion and obtain a unique scaling law relating activity and effective adhesion in both experiments and simulations. Our results provide a new and efficient way to understand the emergence of novel phases of matter in active colloidal suspensions.

  4. Sunset magazine : in search of a house for western living

    E-Print Network [OSTI]

    Gonzalez, Robert Alexander

    1993-01-01

    This thesis inquires into Sunset's activity as a home magazine in the 1930s and 1940s. In viewing this period, this study draws from Sunset's entire history: from its inception in 1898 as a travel brochure for Southern ...

  5. Live Webinar on Better Buildings Case Competition: Energy Efficiency in the Restaurant Franchise Model Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  6. Live Webinar on Better Buildings Case Competition: Taking Commercial PACE Financing to Scale, a Case Study

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webinar titled "A Side of Savings: Energy Efficiency in the Restaurant Franchise Model Case Study."

  7. Semantic Fusion of Live Web Content: System Design and Implementation Experiences

    E-Print Network [OSTI]

    Lenders, Vincent

    Semantic Fusion of Live Web Content: System Design and Implementation Experiences Vincent Lenders armasuisse, Switzerland vincent.lenders@armasuisse.ch Abstract--Conventional Web search models

  8. Living the Information Revolution: Digital Online Culture, Identity & Schooling in the 21st Century

    E-Print Network [OSTI]

    Rosenfeld, Kimberly Nicole

    2013-01-01

    Museum.  (2006a).  Internet  History  -­?  1962-­?1992.  www.computerhistory.org/internet_history/   LIVING  THE  Museum.  (2006b).  Internet  History  -­?  1980’s.  

  9. "We gotta get out of this place": A qualitative study on the effects of leisure travel on the lives of gay men living in a small community 

    E-Print Network [OSTI]

    Herrera, Sergio Lino

    2005-02-17

    A feminist point of view is used in this study of gay men living in a small, collegiate community who use leisure travel as a negotiation strategy to achieve freedom of expression. Feminism is concerned with equality, ...

  10. Identifying Activity

    E-Print Network [OSTI]

    Lewis, Adrian S

    2009-01-01

    Identification of active constraints in constrained optimization is of interest from both practical and theoretical viewpoints, as it holds the promise of reducing an inequality-constrained problem to an equality-constrained problem, in a neighborhood of a solution. We study this issue in the more general setting of composite nonsmooth minimization, in which the objective is a composition of a smooth vector function c with a lower semicontinuous function h, typically nonsmooth but structured. In this setting, the graph of the generalized gradient of h can often be decomposed into a union (nondisjoint) of simpler subsets. "Identification" amounts to deciding which subsets of the graph are "active" in the criticality conditions at a given solution. We give conditions under which any convergent sequence of approximate critical points finitely identifies the activity. Prominent among these properties is a condition akin to the Mangasarian-Fromovitz constraint qualification, which ensures boundedness of the set of...

  11. Structure of Possible Long-lived Asteroid Belts

    E-Print Network [OSTI]

    N. W. Evans; S. A. Tabachnik

    2002-04-24

    High resolution simulations are used to map out the detailed structure of two long-lived stable belts of asteroid orbits in the inner Solar system. The Vulcanoid belt extends from 0.09 to 0.20 astronomical units (au), though with a gaps at 0.15 and 0.18 au corresponding to de-stabilising mean motion resonances with Mercury and Venus. As collisional evolution proceeds slower at larger heliocentric distances, kilometre-sized or larger Vulcanoids are most likely to be found in the region between 0.16 and 0.18 au. The optimum location in which to search for Vulcanoids is at geocentric ecliptic longitudes roughly between 9 and 10 degrees. Dynamically speaking, the Earth-Mars belt between 1.08-1.28 au is an extremely stable repository for asteroids on nearly circular orbits. It is interrupted at 1.21 au due to the 3:4 commensurability with the Earth, while secular resonances with Saturn are troublesome beyond 1.17 au. These detailed maps of the fine structure of the belts can be used to plan search methodologies. Strategies for detecting members of the belts are discussed, including the use of infrared wide-field imaging with VISTA, and forthcoming European Space Agency satellite missions like GAIA and BepiColombo.

  12. Memory Saves Lives: Inter-generational Warnings Effectiveness - 13556

    SciTech Connect (OSTI)

    Van Luik, Abraham; Patterson, Russell [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [U.S. Department of Energy, Carlsbad Field Office, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States); Shafer, David [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States)] [U.S. Department of Energy, Office of Legacy Management, 11025 Dover Street, Suite 1000, Westminster, CO 80021 (United States); Klein, Thomas [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)] [URS Regulatory and Environmental Services, 4021 S. National Parks Highway, Carlsbad, NM 88220 (United States)

    2013-07-01

    The 2011 Tohoku earthquake and tsunami was a world-class natural disaster. It has been described as the most powerful earthquake ever in Japan, and as one of the most powerful earthquakes ever noted in the world. The toll in terms of human lives lost and property destruction was unimaginable. Even the word 'horrible' is inadequate to describe the suffering and misery that resulted. Nations with nuclear power programs are engaged in, or at least planning to become engaged in, arranging to eventually dispose of their higher-level radioactive waste materials in deep geologic repositories. Geologic repositories are passive safety systems, and if undisturbed isolate these dangerous materials form the biosphere for extremely long times. The key words, however, are 'if undisturbed'. To assure that future generations do not inadvertently drill into repositories, national programs, and the international community (the Records, Knowledge and Memory (RK and M) preservation project of the Nuclear Energy Agency, for example), are proposing to place markers and/or monuments on closed repository sites that say 'do not drill here, and this is why' in various sophisticated ways. Such markers or monuments are attempts at providing passive institutional controls. The effectiveness of messages from past generations to a present generation may give an indication of how effective such passive institutional controls may be. (authors)

  13. Search for Long-Lived Particles ine+e-Collisions

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Lees, J.?P.; Poireau, V.; Tisserand, V.; Grauges, E.; Palano, A.; Eigen, G.; Stugu, B.; Brown, D.?N.; Kerth, L.?T.; Kolomensky, Yu.?G.; et al

    2015-04-29

    We present a search for a neutral, long-lived particle L that is produced in e+e- collisions and decays at a significant distance from the e+e- interaction point into various flavor combinations of two oppositely charged tracks. The analysis uses an e+e- data sample with a luminosity of 489.1 fb-1 collected by the BABAR detector at the ? (4S), ? (3S), and ? (2S) resonances and just below the ? (4S). Fitting the two-track mass distribution in search of a signal peak, we do not observe a significant signal, and set 90% confidence level upper limits on the product of themore »L production cross section, branching fraction, and reconstruction efficiency for six possible two-body L decay modes as a function of the L mass. The efficiency is given for each final state as a function of the mass, lifetime, and transverse momentum of the candidate, allowing application of the upper limits to any production model. In addition, upper limits are provided on the branching fraction B(B?XsL), where Xs is a strange hadronic system.« less

  14. Analytical description of anomalous diffusion in living cells

    E-Print Network [OSTI]

    L. Bruno; M. A. Despósito

    2009-04-14

    We propose a stochastic model for intracellular transport processes associated with the activity of molecular motors. This out-of-equilibrium model, based on a generalized Langevin equation, considers a particle immersed in a viscoelastic environment and simultaneously driven by an external random force that models the motors activity. An analytical expression for the mean square displacement is derived, which exhibits a subdiffusive to superdiffusive transition. We show that the experimentally accessible statistical properties of the diffusive particle motion can be reproduced by this model.

  15. Activity report

    SciTech Connect (OSTI)

    Yu, S W

    2008-08-11

    This report is aimed to show the author's activities to support the LDRD. The title is 'Investigation of the Double-C Behavior in the Pu-Ga Time-Temperature-Transformation Diagram' The sections are: (1) Sample Holder Test; (2) Calculation of x-ray diffraction patterns; (3) Literature search and preparing publications; (4) Tasks Required for APS Experiments; and (5) Communications.

  16. Mathematical modeling of positron emission tomography (PET) data to assess radiofluoride transport in living plants following petiolar administration

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Converse, Alexander K.; Ahlers, Elizabeth O.; Bryan, Tom W.; Hetue, Jackson D.; Lake, Katherine A.; Ellison, Paul A.; Engle, Jonathan W.; Barnhart, Todd E.; Nickles, Robert J.; Williams, Paul H.; et al

    2015-03-15

    Background: Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transport in plants after leaf absorption is of interest to those in agricultural areas near industrial sources of airborne fluoride. Here we report the novel use of a commercial, high-resolution, animal positron emission tomography (PET) scanner to trace a bolus of [¹?F]fluoride administered via bisected petioles of Brassica oleracea, an established model species, to simulate whole plant uptake of atmospheric fluoride. This methodology allows for the first time mathematical compartmental modelingmore »of fluoride transport in the living plant. Radiotracer kinetics in the stem were described with a single-parameter free- and trapped-compartment model and mean arrival times at different stem positions were calculated from the free-compartment time-activity curves. Results: After initiation of administration at the bisected leaf stalk, [¹?F] radioactivity climbed for approximately 10 minutes followed by rapid washout from the stem and equilibration within leaves. Kinetic modeling of transport in the stem yielded a trapping rate of 1.5 +/- 0.3%/min (mean +/- s.d., n = 3), velocity of 2.2 +/- 1.1 cm/min, and trapping fraction of 0.8 +/- 0.5%/cm. Conclusion: Quantitative assessment of physiologically meaningful transport parameters of fluoride in living plants is possible using standard positron emission tomography in combination with petiolar radiotracer administration. Movement of free fluoride was observed to be consistent with bulk flow in xylem, namely a rapid and linear change in position with respect to time. Trapping, likely in the apoplast, was observed. Future applications of the methods described here include studies of transport of other ions and molecules of interest in plant physiology.« less

  17. Mathematical modeling of positron emission tomography (PET) data to assess radiofluoride transport in living plants following petiolar administration

    SciTech Connect (OSTI)

    Converse, Alexander K. [Univ. of Wisconsin, Madison, WI (United States); Ahlers, Elizabeth O. [Univ. of Wisconsin, Madison, WI (United States); Bryan, Tom W. [Univ. of Wisconsin, Madison, WI (United States); Hetue, Jackson D. [Univ. of Wisconsin, Madison, WI (United States); Lake, Katherine A. [Univ. of Wisconsin, Madison, WI (United States); Ellison, Paul A. [Univ. of Wisconsin, Madison, WI (United States); Engle, Jonathan W. [Univ. of Wisconsin, Madison, WI (United States); Barnhart, Todd E. [Univ. of Wisconsin, Madison, WI (United States); Nickles, Robert J. [Univ. of Wisconsin, Madison, WI (United States); Williams, Paul H. [Univ. of Wisconsin, Madison, WI (United States); DeJesus, Onofre T. [Univ. of Wisconsin, Madison, WI (United States)

    2015-01-01

    Background: Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transport in plants after leaf absorption is of interest to those in agricultural areas near industrial sources of airborne fluoride. Here we report the novel use of a commercial, high-resolution, animal positron emission tomography (PET) scanner to trace a bolus of [¹?F]fluoride administered via bisected petioles of Brassica oleracea, an established model species, to simulate whole plant uptake of atmospheric fluoride. This methodology allows for the first time mathematical compartmental modeling of fluoride transport in the living plant. Radiotracer kinetics in the stem were described with a single-parameter free- and trapped-compartment model and mean arrival times at different stem positions were calculated from the free-compartment time-activity curves. Results: After initiation of administration at the bisected leaf stalk, [¹?F] radioactivity climbed for approximately 10 minutes followed by rapid washout from the stem and equilibration within leaves. Kinetic modeling of transport in the stem yielded a trapping rate of 1.5 +/- 0.3%/min (mean +/- s.d., n = 3), velocity of 2.2 +/- 1.1 cm/min, and trapping fraction of 0.8 +/- 0.5%/cm. Conclusion: Quantitative assessment of physiologically meaningful transport parameters of fluoride in living plants is possible using standard positron emission tomography in combination with petiolar radiotracer administration. Movement of free fluoride was observed to be consistent with bulk flow in xylem, namely a rapid and linear change in position with respect to time. Trapping, likely in the apoplast, was observed. Future applications of the methods described here include studies of transport of other ions and molecules of interest in plant physiology.

  18. From living cells to atoms Compartmentalisation in the cell

    E-Print Network [OSTI]

    Economou, Tassos

    ;Mitochondrial Energy MetabolismMitochondrial Energy Metabolism #12;The Electrochemical Proton Gradient metabolism enzymes,lipid metabolism enzymes, porinporin IMSIMS 6% of total6% of total mitThe Electrochemical Proton Gradient 140 mV 60 mV (-1 pH unit) TOTAL 200 mV #12;Active transport processes are driven

  19. Protecting Lives and Livelihoods NOAA Business Report 2008

    E-Print Network [OSTI]

    and channels were free of obstructions to allow ship traffic to re- sume service to these areas. NOAA also an estimated 419,000 gallons of No. 6 fuel oil spilled in the Mississippi River, just north of New Orleans active aiding in responding to the spill of an estimated 58,000 gallons of intermediate fuel oil, which

  20. LIVE CARS FOR USE IN CATFISH INDUSTRY Donald C. ,r nland, Rob rt L. ..rill,

    E-Print Network [OSTI]

    LIVE CARS FOR USE IN CATFISH INDUSTRY Donald C. ,r nland, Rob rt L. ..rill, & Jam's . IIall Live cars - -m sh "fish -holdmg bags "- -hav a vari tyof applications in th produ tion of pond - rai d chann fish to mov into th liv car . Information on holding capaciti and a m thod to accu- rat ly m t l' fish

  1. Nature Macmillan Publishers Ltd 1997 insulating; and so on. A living polymer-

    E-Print Network [OSTI]

    Nature © Macmillan Publishers Ltd 1997 insulating; and so on. A living polymer- ization method . David A. Tirrell is in the Department of Polymer Science and Engineering, University of Massachusetts. Biopolymers 16, 2005­2028 (1977). 7. Szwarc, M. Carbanions, Living Polymers, and Electron-Transfer Processes

  2. The LivingKnowledge Project: Exploring the Spectrum of Opinions over Time

    E-Print Network [OSTI]

    Moschitti, Alessandro

    The LivingKnowledge Project: Exploring the Spectrum of Opinions over Time Richard Johansson-of-words. In contrast, recent research in sentiment classification has shown that, when the required semantic is crucial. In this perspective, the LivingKnowledge (LK) project, funded by the seventh EU framework program

  3. Run-time Modification of the Class Hierarchy in a Live Java Development Environment

    E-Print Network [OSTI]

    Goldman, Kenneth J.

    1 Run-time Modification of the Class Hierarchy in a Live Java Development Environment Joel R. Louis {jbrandt, kjg}@wustl.edu Abstract Class hierarchy design is central to object-oriented software work on live development environments to allow run-time modification of the class hierarchy. The result

  4. Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer

    E-Print Network [OSTI]

    Fischer, Gerhard

    Tools for Living and Tools for Learning Stefan Carmien and Gerhard Fischer University of Colorado as tools, particularly computational artifacts. We introduce the notions of tools for living and tools for the design of artifacts. The tools concept is then studied in the context of the Memory Aiding Prompting

  5. How did LUCA make a living? Chemiosmosis in the origin of life

    E-Print Network [OSTI]

    Allen, John F.

    necessary for carbon and energy metabolism in all free-living chemotrophs, and presum- ably the first free-living cells too. Proton gradients form naturally at alkaline hydrothermal vents and are viewed as central from the vents. Synthesis of ATP by chemi- osmosis today involves generation of an ion gradient

  6. Modeling Computational Security in Long-Lived Systems, Version 2 Ran Canetti1,2

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    Modeling Computational Security in Long-Lived Systems, Version 2 Ran Canetti1,2 , Ling Cheung2 Introduction Computational security in long-lived systems: Security properties of cryptographic protocols computational power. This type of security degrades progressively over the lifetime of a protocol. However, some

  7. Modeling Computational Security in Long-Lived Systems Ran Canetti1,2

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    Modeling Computational Security in Long-Lived Systems Ran Canetti1,2 , Ling Cheung2 , Dilsun Kaynar Introduction Computational security in long-lived systems: Security properties of cryptographic protocols protocols, security relies on the assumption that adversarial entities have lim- ited computational power

  8. Peer-to-peer live streaming for massively multiplayer online games

    E-Print Network [OSTI]

    ). The survey we conducted in the context of the FP7-funded CNG project [1] has highlighted that streaming live of a few friends and teammates. Within the CNG project, we have developed a P2P system for sharing live

  9. International Series of Numerical Mathematics, Vol. 160, 521540 Freezing of Living Cells: Mathematical Models

    E-Print Network [OSTI]

    Turova, Varvara

    International Series of Numerical Mathematics, Vol. 160, 521­540 Freezing of Living Cells, stresses arising due to non-simultaneous freezing of water in- side and outside of cells are modeled and outside of living cells during freezing is derived by applying an appropriate averaging technique

  10. More Indian River residents living below poverty level, Census report says

    E-Print Network [OSTI]

    Fernandez, Eduardo

    More Indian River residents living below poverty level, Census report says By Keona Gardner Thursday, September 22, 2011 INDIAN RIVER COUNTY -- The county's poverty level is at a three-year high had 14.6 percent of its residents living below the poverty level, compared with 9.5 percent in 2007

  11. Transforming systems to improve lives. Health and Humanitarian Supply Chain Management

    E-Print Network [OSTI]

    Li, Mo

    in unintended consequences, including the loss of lives. These challenging logistics and supply chain management taken a course offered by the GT Supply Chain & Logistics Institute. To learn about qualifyingTransforming systems to improve lives. Health and Humanitarian Supply Chain Management

  12. Capsules and Semantic Regions for Code Visualization and Direct Manipulation of Live Programs

    E-Print Network [OSTI]

    Goldman, Kenneth J.

    in an educational setting. Because programs are edited live, editing gestures must transform the program from one of JPie programs and support live program editing through natural atomic gestures. Categories and Subject the program. JPie users edit programs by manipulating a visualization of this executable data structure

  13. The efficiency of mitochondrial electron transport chain is increased in the long-lived mrg19

    E-Print Network [OSTI]

    Babu, M. Madan

    The efficiency of mitochondrial electron transport chain is increased in the long-lived mrg19 mtROS and contribute to longevity. This increased mitochondrial efficiency (i.e. low mtROS generated the observed higher mito- chondrial efficiency in the long-lived mrg19 mutant of Saccharomyces cerevisiae

  14. FUTURE URBAN LIVING A policy commission investigating the most appropriate means for

    E-Print Network [OSTI]

    Birmingham, University of

    FUTURE URBAN LIVING A policy commission investigating the most appropriate means for accommodating changing populations and their needs in the cities of the future The Report 2014 #12;2 Birmingham Policy Commission on Future Urban Living Foreword by the Chair of the Policy Commission I am delighted to have been

  15. Volume 23 November 1, 2012 4165 Living matter--nexus of physics and biology

    E-Print Network [OSTI]

    Gardel, Margaret

    Volume 23 November 1, 2012 4165 Living matter--nexus of physics and biology in the 21st century Margaret L. Gardel Institute for Biophysical Dynamics, James Franck Institute, and Department of Physics tissue architecture and mechanics. Elucidating the underlying physical principles of such living matter

  16. Sex Differences and Attitudes toward Living Donor Kidney Transplantation among Urban Black Patients on

    E-Print Network [OSTI]

    Obradovic, Zoran

    Article Sex Differences and Attitudes toward Living Donor Kidney Transplantation among Urban Black by women and blacks. To better understand sex differences in the context of potential barriers to living for a kidney transplant (28.3% versus 52.2%, P=0.01). The multiple logistic regression analysis showed that sex

  17. Queuing Network Models for Multi-Channel P2P Live Streaming Systems

    E-Print Network [OSTI]

    Liu, Yong

    , including peer channel switching, peer churn, peer bandwidth heterogeneity, and Zipf-like channel popularity if not millions of live channels. In P2P live video streaming systems, churn occurs on two different time scales-channel or more simply as ISO P2P streaming systems. Unfortunately, this channel churn brings enormous instability

  18. What price a roof? Housing and the cost of living in 16th-century Toledo*

    E-Print Network [OSTI]

    What price a roof? Housing and the cost of living in 16th-century Toledo* Mauricio Drelichman market. We then explore the impact of adding rent to early modern price indices and estimates of living standards. Price indices show a moderate effect. The addition of rent reduces the gap between Toledo and two

  19. Modeling magnetosensitive ion channels in viscoelastic environment of living cells

    E-Print Network [OSTI]

    Igor Goychuk

    2015-10-01

    We propose and study a model of hypothetical magnetosensitive ionic channels which are long thought to be a possible candidate to explain the influence of weak magnetic fields on living organisms ranging from magnetotactic bacteria to fishes, birds, rats, bats and other mammals including humans. The core of the model is provided by a short chain of magnetosomes serving as a sensor which is coupled by elastic linkers to the gating elements of ion channels forming a small cluster in the cell membrane. The magnetic sensor is fixed by one end on cytoskeleton elements attached to the membrane and is exposed to viscoelastic cytosol. Its free end can reorient stochastically and subdiffusively in viscoelastic cytosol responding to external magnetic field changes and open the gates of coupled ion channels. The sensor dynamics is generally bistable due to bistability of the gates which can be in two states with probabilities which depend on the sensor orientation. For realistic parameters, it is shown that this model channel can operate in the magnetic field of Earth for a small number (5 to 7) of single-domain magnetosomes constituting the sensor rod each of which has a typical size found in magnetotactic bacteria and other organisms, or even just one sufficiently large nanoparticle of a characteristic size also found in nature. It is shown that due to viscoelasticity of medium the bistable gating dynamics generally exhibits power law and stretched exponential distributions of the residence times of the channels in their open and closed states. This provides a generic physical mechanism for explanation of the origin of such anomalous kinetics for other ionic channels whose sensors move in viscoelastic environment provided by either cytosol or biological membrane, in a quite general context, beyond the fascinating hypothesis of magnetosensitive ionic channels we explore.

  20. Laboratory Activities

    SciTech Connect (OSTI)

    Brown, Christopher F.; Serne, R. Jeffrey

    2008-01-17

    This chapter summarizes the laboratory activities performed by PNNL’s Vadose Zone Characterization Project in support of the Tank Farm Vadose Zone Program, led by CH2M HILL Hanford Group, Inc. The results of these studies are contained in numerous reports (Lindenmeier et al. 2002; Serne et al. 2002a, 2002b, 2002c, 2002d, 2002e; Lindenmeier et al. 2003; Serne et al. 2004a, 2004b; Brown et al. 2005, 2006a, 2007; Serne et al. 2007) and have generated much of the data reported in Chapter 22 (Geochemistry-Contaminant Movement), Appendix G (Geochemistry-Contaminant Movement), and Cantrell et al. (2007, SST WMA Geochemistry Data Package – in preparation). Sediment samples and characterization results from PNNL’s Vadose Zone Characterization Project are also shared with other science and technology (S&T) research projects, such as those summarized in Chapter 12 (Associated Science Activities).

  1. Portsmouth Educational Outreach Seeks to Transform Lives of Area Students |

    Energy Savers [EERE]

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on DeliciousMathematics AndBeryllium Disease | DepartmentOLEDEnergy Political Activity at DOE FacilitiesDepartment of

  2. Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

    SciTech Connect (OSTI)

    Kerkhoff, Lee; Williams, Kenneth H.; Long, Philip E.; McGuinness, L.

    2011-02-15

    Uranium contaminated groundwaters are a legacy concern for the U.S. Department of Energy. Previous experiments at the Rifle, Colorado Integrated Field Challenge (IFC) site have demonstrated that field-scale addition of acetate to groundwater reduces the ambient soluable uranium concentration, sequestering the radionuclide as uraninite. However, questions remain regarding which microorganism(s) are consuming this acetate and if active groundwater microorganisms are different from active particle-associated bacteria. In this report, 13-C acetate was used to assess the active microbes that synthesize DNA on 3 size fractions [coarse sand, fines (8-approximately 150 micron), groundwater (0.2-8 micron)] over a 24 -day time frame. Results indicated a stronger signal from 13-C acetate associated with the “fines” fraction compared with smaller amounts of 13-C uptake on the sand fraction and groundwater samples during the SIP incubations. TRFLP analysis of this 13-C-labeled DNA, indicated 31+ 9 OTU's with 6 peaks dominating the active profiles (166, 187, 210, 212, and 277 bp peaks using MnlI). Cloning/sequencing of the amplification products indicated a Geobacter-like group (187, 210, 212 bp) primarily synthesized DNA from acetate in the groundwater phase, an alpha Proteobacterium (166 bp) primarily grew on the fines/sands, and an Acinetobacter sp. (277 bp) utilized much of the 13C acetate in both groundwater and particle-associated phases. These findings will help to delineate the acetate utilization patterns of bacteria during field-scale acetate addition and can lead to improved methods for stimulating distinct microbial populations in situ.

  3. A solution to the subdiffusion-efficiency paradox: Inactive states enhance reaction efficiency at subdiffusion conditions in living cells

    E-Print Network [OSTI]

    Leila Esmaeili Sereshki; Michael A. Lomholt; Ralf Metzler

    2012-03-08

    Macromolecular crowding in living biological cells effects subdiffusion of larger biomolecules such as proteins and enzymes. Mimicking this subdiffusion in terms of random walks on a critical percolation cluster, we here present a case study of EcoRV restriction enzymes involved in vital cellular defence. We show that due to its so far elusive propensity to an inactive state the enzyme avoids non-specific binding and remains well-distributed in the bulk cytoplasm of the cell. Despite the reduced volume exploration capability of subdiffusion processes, this mechanism guarantees a high efficiency of the enzyme. By variation of the non-specific binding constant and the bond occupation probability on the percolation network, we demonstrate that reduced non-specific binding are beneficial for efficient subdiffusive enzyme activity even in relatively small bacteria cells. Our results corroborate a more local picture of cellular regulation.

  4. The Quick Fix: Napping | Living Healthy | Reader's Digest http://www.rd.com.edgekey.net/living-healthy/the-quick-fix-napping-slee... 1 of 3 6/5/2008 5:17 PM

    E-Print Network [OSTI]

    Mednick, Sara C.

    The Quick Fix: Napping | Living Healthy | Reader's Digest http://www.rd.com Make Your Mark #12;The Quick Fix: Napping | Living Healthy | Reader's Digest http://www.rd.com Text SizeBookmark Yahoo! Buzz #12;The Quick Fix: Napping | Living Healthy | Reader's Digest http://www

  5. American Legacy Foundation. Saving Lives, Saving Money: Why States Should Invest in a Tobacco-Free Future.

    E-Print Network [OSTI]

    Paul G. Billings; Frank Chaloupka; Matthrew Farrelly; Eric N. Lindblom; Thomas Novotny

    2002-01-01

    SAVINGLIVES, SAVING MONEY. Why States Should Invest in a Tobacco-American Legacy Foundation. Saving Lives, Saving Money: Why

  6. 110. Location efficiency Discovering the hidden transportation costs of where you live page 1 / 1 110. Location efficiency

    E-Print Network [OSTI]

    Machel, Hans

    110. Location efficiency Discovering the hidden transportation costs of where you live page 1 / 1 110. Location efficiency: Discovering the hidden transportation costs of where you live Live location efficiency and it's the amount of time, energy and greenhouse gas emissions you spend getting

  7. "Architectural Robotics": An Interdisciplinary Course Rethinking the Machines We Live In

    E-Print Network [OSTI]

    "Architectural Robotics": An Interdisciplinary Course Rethinking the Machines We Live In Apoorva- ditionally prevented robotics from significantly impacting the built (architectural) environment we inhabit-level course in Architectural Robotics. The results from class interactions and projects provide insight

  8. M49-- Machine for the Living : a performance broadcast through an interfering FM radio transmission

    E-Print Network [OSTI]

    Goldfarb, Maximilian M

    2006-01-01

    The focus of the M49: Machine For The Living project is a performance work that considers the omnipresence of layered communications, which extend, yet supersede corporeal space of the individual. M49 creates a framework ...

  9. Search for Long-Lived Particles in e[superscript +]e[superscript -] Collisions

    E-Print Network [OSTI]

    Lees, J.?P.

    We present a search for a neutral, long-lived particle L that is produced in e[superscript +]e[superscript -] collisions and decays at a significant distance from the e[superscript +]e[superscript -] interaction point into ...

  10. ESA living planet symposium 28 June 2 July 2010, Bergen, Norway

    E-Print Network [OSTI]

    Stuttgart, Universität

    of unobserved GGs Stochastic model ESA living planet symposium 2010 Stochastic model High performance computing estimation stochastic model of invariants high performance computing error propagation synthesis performance computing environment Oliver Baur, Nico Sneeuw, Jianqing Cai, Matthias Roth Institute of Geodesy

  11. Using Schwartz Values to Assess Stakeholder Relations in the Coigach-Assynt Living Landscape 

    E-Print Network [OSTI]

    Burgener, Ariana Jude

    2014-11-27

    This dissertation explores stakeholder relations in an environmental management case study from Northwest Scotland that has aimed to employ the Ecosystem Approach in their methods. The Coigach-Assynt Living Landscape ...

  12. Delivery of proteins in live cells with viral peptides: principles and mechanisms 

    E-Print Network [OSTI]

    Lee, Ya-Jung

    2012-07-16

    Cell-penetrating peptides (CPPs) mediate the delivery of macromolecules across the plasma membrane of live cells. These peptides are therefore important due to the potential of making the delivery of protein probes or therapeutics a routine...

  13. The Role of Acculturation in Nutrition Behaviors among Low Income Hispanic Women Living in Texas 

    E-Print Network [OSTI]

    Atehortua, Nelson

    2012-10-19

    The purpose of this study was to determine the role of acculturation in the food consumption patterns of low income Hispanic women living in Texas and enrolled in the Women, Infants, and Children (WIC) Program by testing ...

  14. Live Webcast on the 2014 Farm Bill's Renewable Energy for America Program

    Broader source: Energy.gov [DOE]

    The Energy Department will present a live webcast titled “The 2014 Farm Bill's Renewable Energy for America Program” on May 21, 2014, from 3:00 to 4:00 p.m. Eastern Standard Time.

  15. Federal Poverty Guideline Underestimates Costs of Living for Older Persons in California

    E-Print Network [OSTI]

    Wallace, Steven P.; Molina, L. Cricel

    2008-01-01

    options. Food costs in the Elder Index are based on the U.S.Elder Index for California takes into account actual costsElder Index provides a calculation of a basic cost of living

  16. New reporters of protein trafficking and protein-protein interactions in live cells

    E-Print Network [OSTI]

    Fernández Suárez, Marta

    2008-01-01

    Here, we describe our attempts to harness the exquisite specificity of natural protein and RNA enzymes to develop improved methods to study protein localization and protein-protein interactions in live cells. We first ...

  17. Live Webinar on the Funding Opportunity for Wind Forecasting Improvement Project in Complex Terrain

    Broader source: Energy.gov [DOE]

    On April 21, 2014 from 3:00 to 5:00 PM EST the Wind Program will hold a live webinar to provide information to potential applicants for this Funding Opportunity Announcement. There is no cost to...

  18. HOMEOFF-CAMPUS LIVING GUIDE DLORD AMENITIES UTILITIES LEASE CLAUSE TENANT MOVE

    E-Print Network [OSTI]

    HOMEOFF-CAMPUS LIVING GUIDE #12;DLORD · AMENITIES · UTILITIES · LEASE · CLAUSE · TENANT · MOVE · AMENITIES · UTILITIES · LEASE · CLAUSE ANT · MOVE · FURNISHED · BATHROOM · FREEDOM · SHUTTLE · OCCU CY UNIT · PROPE COMPLEX · HOME · CLUBHOUSE · ROOMMATE · BEDROOM · LANDLO ENITIES · UTILITIES · LEASE

  19. Search for long-lived particles that decay into final states...

    Office of Scientific and Technical Information (OSTI)

    obtained are the most stringent to date. In the specific case of a model in which a Higgs boson in the mass range 125-1000mathrmGeVc2 decays into a pair of long-lived...

  20. Use of encapsulated live microalgae to investigate pre-ingestive selection in the oyster Crassostrea gigas

    E-Print Network [OSTI]

    Use of encapsulated live microalgae to investigate pre-ingestive selection in the oyster, this study clearly demonstrate that extracellular metabolites produced by microalgae play a crucial role reserved. Keywords: Alginate; Beads; Bivalve; Feeding; Microalgae; Particle processing 1. Introduction

  1. Synthetic mimics of mammalian cell surface receptors: prosthetic molecules that augment living cells

    E-Print Network [OSTI]

    Peterson, Blake R.

    2005-09-08

    -alkyl derivatives of 3?-cholesterylamine linked to motifs that bind cell-impermeable ligands. When added to living mammalian cells, these synthetic receptors insert into cellular plasma membranes, project ligand-binding small molecules or peptides from the cell...

  2. On the "counter-example" in the article "Max -controlled siphons for liveness of S3

    E-Print Network [OSTI]

    Reveliotis, Spiridon "Spyros"

    On the "counter-example" in the article "Max -controlled siphons for liveness of S3 PGR2 characterization of the S3PGR2 nets by means of the structural object of deadly marked siphon, are correct

  3. Sustainability processes in community-level health initiatives: the experiences of Scottish healthy living centres 

    E-Print Network [OSTI]

    Rankin, David

    2010-01-01

    Background This thesis explores processes involved in stakeholders’ attempts to secure sustainability of three short-term funded community health initiatives known as healthy living centres (HLCs). The overall aim was ...

  4. U.S. Bamboo house of the future : standardizing ecological living

    E-Print Network [OSTI]

    Wong, Lucy Lai

    2006-01-01

    This thesis focuses on ecological living through the use of bamboo. It explores how the material can be used for methods of prefabricated housing design within the United States. It also uses a "ht of parts" and describes ...

  5. Working lives of prison managers: exploring agency and structure in the late modern prison 

    E-Print Network [OSTI]

    Bennett, Jamie Stewart

    2012-11-27

    This study explores the contemporary working lives of prison managers. It attempts to understand the ways in which globalised changes in management practices have intersected with localised practices and occupational ...

  6. No longer living together: how does Scots cohabitation law work in practice? 

    E-Print Network [OSTI]

    Wasoff, Fran; Miles, Jo; Mordaunt, Edith

    In response to the greater diversity of family life in Scotland, the Family Law (Scotland) Act 2006 gives some recognition to people who live together without marriage or civil partnership and provides limited financial ...

  7. Balance-guaranteed optimized tree with reject option for live fish recognition 

    E-Print Network [OSTI]

    Huang, Xuan; Huang, Phoenix X.

    2014-11-27

    This thesis investigates the computer vision application of live fish recognition, which is needed in application scenarios where manual annotation is too expensive, when there are too many underwater videos. This system ...

  8. Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging

    E-Print Network [OSTI]

    Martell, Jeffrey Daniel

    Microscopy and mass spectrometry (MS) are complementary techniques: The former provides spatiotemporal information in living cells, but only for a handful of recombinant proteins at a time, whereas the latter can detect ...

  9. Live Webinar on the Funding Opportunity for Marine and Hydrokinetic Research and Development University Consortium

    Office of Energy Efficiency and Renewable Energy (EERE)

    On April 24, 2014 from 1:00 - 2:30 PM EDT, the Water Power Program will hold a live webinar to provide information to potential applicants for the Marine and Hydrokinetic (MHK) Research and...

  10. Making lives under closure : birth and medicine in Palestine's waiting zones

    E-Print Network [OSTI]

    Wick, Livia

    2006-01-01

    Reproduction is a site for understanding the ways in which people reconceptualize and re-organize the world in which they live. This dissertation tries to understand the world of birth under the regime of closures and ...

  11. Effects of carbohydrate applications on growth and vitality of live oak (Quercus virginiana) 

    E-Print Network [OSTI]

    Martinez Trinidad, Tomas

    2009-05-15

    Urban forests grow in stressful environments that can have negativerepercussions on tree energy reserves. The goal of this research was to evaluate theimpact of exogenously applied carbohydrates on growth and vitality of live oaks(Quercus virginiana...

  12. Sipping fuel and saving lives: increasing fuel economy without sacrificing safety

    E-Print Network [OSTI]

    Gordon, Deborah; Greene, David L.; Ross, Marc H.; Wenzel, Tom P.

    2008-01-01

    Ford delays plans to boost fuel economy of its SUVs. WallImproving safety without impacting fuel economy. Honda MotorCompany, October 4. SIPPING FUEL AND SAVING LIVES / 24

  13. The potential use of thiabendazole and propiconazole for oak wilt control in live oak 

    E-Print Network [OSTI]

    Roberts, Paul Edwin

    1988-01-01

    analysis with a variable acceptance level of 0. 15. 28 Models tested for xylem pressure potential, using stepwise regression analysis with a variable acceptance level of 0. 15. . . . . . . . . . . . 29 Phytotoxicity rating of live oaks injected...THE POTENTIAL USE OF THIABENDAZOLE AND PROPICONAZOLE FOR OAK WILT CONTROL IN LIVE OAK A Thesis by PAUL EDWIN ROBERTS Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER...

  14. Distribution and a possible mechanism of uranium accumulation in the Catahoula Tuff, Live Oak County, Texas 

    E-Print Network [OSTI]

    Parks, Steven Louis

    1979-01-01

    DISTRIBUTION AND A POSSIBLE MECHANISM OF URANIUM ACCUMULATION IN THE CATAHOULA TUFF, LIVE OAK COUNTY, TEXAS A Thesis by Steven Louis Parks Submitted to the Graduate College of Texas ARM University in partial fulfillment of the requirements... f' or the degree of MASTER OF SCIENCE May 1979 Major Subject: Geology DISTRIBUTION AND A POSSIBLE MECHANISM OF URANIUM ACCUMULATION IN THE CATAHOULA TUFF, LIVE OAK COUNTY, TEXAS A Thesis by Steven Louis Parks Approved as to style...

  15. Quantifying CO2 removal by living walls: a case study of the Center for Design Research

    E-Print Network [OSTI]

    Rivera, Eric

    2014-04-01

    20 | JOURNAL OF UNDERGRADUATE RESEARCH Quantifying CO2 removal by living walls: a case study of the Center for Design Research Eric Rivera Q&A How did you become involved in doing research? I became interested in research through the Mc... to improve IAQ, located at the University of Kansas. This study investigated the effectiveness of the living wall in reducing carbon dioxide (CO2) concentration levels indoors, as well as the impact the mechanical system has in reducing CO2 concentration...

  16. Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° north using ¹³C DNA stable isotope probing and catalyzed activated reporter deposition-fluorescence in situ hybridization

    E-Print Network [OSTI]

    Richberg, Kevin Patrick

    2010-01-01

    At deep-sea hydrothermal vents chemolithoautotrophic microbes mediate the transfer of geothermal chemical energy to higher trophic levels. To better understand these underlying processes and the organisms catalyzing them, ...

  17. ‘Our Tables Have Suffered’: Quantifying consumer market activity of commercially valuable living resources in Chesapeake Bay, 1850-1950 

    E-Print Network [OSTI]

    Carter, Joshua

    2013-02-04

    important commercial species and their decline and/or withdrawal from food markets for periods prior to the 1950 establishment of NOAA fisheries catch data. We examined data sources, including historical newspapers, price current lists, and menus to capture...

  18. Global and regional evolution of short-lived radiatively-active gases and aerosols in the Representative Concentration Pathways

    SciTech Connect (OSTI)

    Lamarque, J.-F.; Kyle, G. Page; Meinshausen, Malte; Riahi, Keywan; Smith, Steven J.; Van Vuuren, Detlef; Conley, Andrew; Vitt, Francis

    2011-08-05

    In this paper, we discuss the results of 2000-2100 simulations with a chemistry-climate model, focusing on the changes in atmospheric composition (troposphere and stratosphere) following the emissions associated with the Representative Concentration Pathways. We show that tropospheric ozone is projected to decrease (RCP3PD and RCP4.5) or increase (RCP8.5) between 2000 and 2100. Surface ozone in 2100 is projected to change little compared from 2000 conditions, a much-reduced impact from the projections based on the A2 scenario. Aerosols are projected to strongly decrease in the 21st century, a reflection of their projected decrease in emissions. Similarly, sulfate deposition is projected to strongly decrease. However, nitrogen deposition is projected to increase over certain regions because of the projected increase NH3 emissions.

  19. Nanoscopy in a Living Multicellular Organism Expressing GFP Brian R. Rankin, Gael Moneron, Christian A. Wurm, Jessica C. Nelson, Arne Walter, Dirk Schwarzer,{

    E-Print Network [OSTI]

    Hell, Stefan W.

    Nanoscopy in a Living Multicellular Organism Expressing GFP Brian R. Rankin, Gael Moneron living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP system of an intact, living, multicellular organism. We determine that the spectral range suitable

  20. Bumblebee vibration activated foraging

    E-Print Network [OSTI]

    Su, Dan Kuan-Nien

    2009-01-01

    SAN DIEGO Bumblebee vibration activated foraging: A ThesisOF THE THESIS Bumblebee vibration activated foraging by Danproduce brief bursts of vibration (foraging activation

  1. Intermediate Energy Infobook Activities (29 Activities)

    K-12 Energy Lesson Plans and Activities Web site (EERE)

    Information about Intermediate Energy Infobook, 29 student activities on energy basics for grades 5-8.

  2. Uranium Biomineralization by Natural Microbial Phosphatase Activities in the Subsurface

    SciTech Connect (OSTI)

    Sobecky, Patricia A.

    2015-04-06

    In this project, inter-disciplinary research activities were conducted in collaboration among investigators at The University of Alabama (UA), Georgia Institute of Technology (GT), Lawrence Berkeley National Laboratory (LBNL), Brookhaven National Laboratory (BNL), the DOE Joint Genome Institute (JGI), and the Stanford Synchrotron Radiation Light source (SSRL) to: (i) confirm that phosphatase activities of subsurface bacteria in Area 2 and 3 from the Oak Ridge Field Research Center result in solid U-phosphate precipitation in aerobic and anaerobic conditions; (ii) investigate the eventual competition between uranium biomineralization via U-phosphate precipitation and uranium bioreduction; (iii) determine subsurface microbial community structure changes of Area 2 soils following organophosphate amendments; (iv) obtain the complete genome sequences of the Rahnella sp. Y9-602 and the type-strain Rahnella aquatilis ATCC 33071 isolated from these soils; (v) determine if polyphosphate accumulation and phytate hydrolysis can be used to promote U(VI) biomineralization in subsurface sediments; (vi) characterize the effect of uranium on phytate hydrolysis by a new microorganism isolated from uranium-contaminated sediments; (vii) utilize positron-emission tomography to label and track metabolically-active bacteria in soil columns, and (viii) study the stability of the uranium phosphate mineral product. Microarray analyses and mineral precipitation characterizations were conducted in collaboration with DOE SBR-funded investigators at LBNL. Thus, microbial phosphorus metabolism has been shown to have a contributing role to uranium immobilization in the subsurface.

  3. Assessing the Quality of Activities in a Smart Environment D.J. Cook and M. Schmitter-Edgecombe

    E-Print Network [OSTI]

    Cook, Diane J.

    are used to recognize activities that are performed in a smart home and to identify errors using machine learning algorithms and smart home technology. These algorithms will be useful for automating remote health monitoring and interventions. Keywords: activities of daily living, smart homes

  4. Retrospective radon progeny measurements through measurements of 210 activities on glass objects using stacked LR 115 detectors

    E-Print Network [OSTI]

    Yu, Peter K.N.

    Retrospective radon progeny measurements through measurements of 210 Po activities on glass objects 2 September 2008 PACS: 29.40 23.60 Keywords: Radon Radon progeny 210 Po Implantation Retrospective for determining 210 Po activity in glass surfaces after deposition of short-lived radon progeny. The sensitivities

  5. Systematic calculations of $?$-decay half-lives with an improved empirical formula

    E-Print Network [OSTI]

    Z. Y. Wang; Z. M. Niu; Q. Liu; J. Y. Guo

    2015-03-05

    Based on the recent data in NUBASE2012, an improved empirical formula for evaluating the $\\alpha$-decay half-lives is presented, in which the hindrance effect resulted from the change of the ground state spins and parities of parent and daughter nuclei is included, together with a new correction factor for nuclei near the shell closures. The calculated $\\alpha$-decay half-lives are found to be in better agreements with the experimental data, and the corresponding root-mean-square (rms) deviation is reduced to $0.433$ when the experimental $Q$-values are employed. Furthermore, the $Q$-values derived from different nuclear mass models are used to predict $\\alpha$-decay half-lives with this improved formula. It is found that the calculated half-lives are very sensitive to the $Q$-values. Remarkably, when mass predictions are improved with the radial basis function (RBF), the resulting rms deviations can be significantly reduced. With the mass prediction from the latest version of Weizs\\"{a}cker-Skyrme (WS4) model, the rms deviation of $\\alpha$-decay half-lives with respect to the known data falls to $0.697$.

  6. Half-lives of $\\alpha$ decay from natural nuclides and from superheavy elements

    E-Print Network [OSTI]

    Qian, Yibin

    2014-01-01

    Recently, experimental researches on the $\\alpha$ decay with long lifetime are one of hot topics in the contemporary nuclear physics [e.g. N. Kinoshita {\\sl et al.} (2012) and J. W. Beeman {\\sl et al.} (2012) ]. In this study, we have systematically investigated the extremely long-lived $\\alpha$-decaying nuclei within a generalized density-dependent cluster model involving the experimental nuclear charge radii. In detail, the important density distribution of daughter nuclei is deduced from the corresponding experimental charge radii, leading to an improved $\\alpha$-core potential in the quantum tunneling calculation of $\\alpha$-decay width. Besides the excellent agreement between theory and experiment, predictions on half-lives of possible candidates for natural $\\alpha$ emitters are made for future experimental detections. In addition, the recently confirmed $\\alpha$-decay chain from $^{294}$117 is well described, including the attractive long-lived $\\alpha$-decaying $^{270}$Db, i.e., a positive step toward...

  7. Secondary Energy Infobook Activities (19 Activities)

    K-12 Energy Lesson Plans and Activities Web site (EERE)

    Information about Secondary Energy Infobook, 19 student activities on energy basics for grades 5-8 and 9-12.

  8. Area Activation 1 Running Head: AREA ACTIVATION

    E-Print Network [OSTI]

    Pomplun, Marc

    Area Activation 1 Running Head: AREA ACTIVATION Advancing Area Activation towards a General Model at Boston 100 Morrissey Boulevard Boston, MA 02125-3393 USA Phone: 617-287-6485 Fax: 617-287-6433 e. Without great effort, human observers clearly outperform every current artificial vision system in tasks

  9. Harvard-MIT research program in short-lived radiopharmaceuticals. Progress report, September 1, 1979-February 28, 1981. [Lead abstract

    SciTech Connect (OSTI)

    Adelstein, S. J.; Brownell, G. L.

    1980-10-01

    Separate abstracts were prepared for the seven progress reports of the Harvard-MIT research program in short-lived radiopharmaceuticals. (KRM)

  10. Extraction of short-lived zirconium and hafnium isotopes using crown ethers: A model system for the study of rutherfordium

    E-Print Network [OSTI]

    2005-01-01

    Extraction of short-lived zirconium and hafnium isotopesReceived: ; Accepted: Zirconium / Hafnium / Crown ether /The extraction of zirconium and hafnium from hydrochloric

  11. Activity Based Costing

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1997-03-28

    Activity Based Costing (ABC) is method for developing cost estimates in which the project is subdivided into discrete, quantifiable activities or a work unit. This chapter outlines the Activity Based Costing method and discusses applicable uses of ABC.

  12. LiveLab: Measuring Wireless Networks and Smartphone Users in the Field

    E-Print Network [OSTI]

    Zhong, Lin

    LiveLab: Measuring Wireless Networks and Smartphone Users in the Field Clayton Shepard*, Ahmad and wireless networks with a reprogrammable in- device logger designed for long-term user studies. We discuss of smartphone usage and measurement of wireless networks x In-field programmability of the logger so

  13. LiveLab: Measuring Wireless Networks and Smartphone Users in the Field

    E-Print Network [OSTI]

    Zhong, Lin

    1 LiveLab: Measuring Wireless Networks and Smartphone Users in the Field Clayton Shepard*, Ahmad and wireless networks with a reprogrammable in- device logger designed for long-term user studies. We discuss to measure smartphone users in the field and to measure wireless networks with smartphone users. The key

  14. In Vivo Optical Microendoscopy for Imaging Cells Lying Deep within Live Tissue

    E-Print Network [OSTI]

    Schnitzer, Mark

    1 50 In Vivo Optical Microendoscopy for Imaging Cells Lying Deep within Live Tissue ABSTRACT hippocampal area, but our general approach is broadly applicable to other deep brain regions and areas by standard water-immersion microscope objectives. Microendoscopes are also compatible with chronic animal

  15. Effects of Shade on Blue Oak and Coast Live Oak Regeneration in California Annual Grasslands1

    E-Print Network [OSTI]

    Effects of Shade on Blue Oak and Coast Live Oak Regeneration in California Annual Grasslands1 manipulation. A split-plot, multifactorial experi ment was designed to test whether blue oak (Quercus douglasii herbaceous vegetation was not manipulated. Even under conditions of prolonged, severe drought, blue oaks

  16. Composting 101 Prepared by the HLS Green Team and Green Living Program, updated February 2011

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Composting 101 Prepared by the HLS Green Team and Green Living Program, updated February 2011.5% was composted. The environmental effect of NOT trashing this mass would be equivalent to removing 5.8 million cars from the road. (www.epa.gov)1 What is Compost? Compost is the product of a biological process

  17. Composting at HLS Brought to you by the Green Team and Green Living Program February 2011

    E-Print Network [OSTI]

    Wolfe, Patrick J.

    Composting at HLS Brought to you by the Green Team and Green Living Program February 2011 Americans.5% was composted. The environmental effect of NOT trashing this mass would be equivalent to removing 5.8 million cars from the road. (www.epa.gov)1 Compost Basics Why Compost? 1 Emissions avoidance from compost

  18. Remarks on the statistical study of protein-protein interaction in living cells

    E-Print Network [OSTI]

    Ph. Heinrich; J. Kahn; L. Héliot; D. Trinel

    2011-05-28

    In this note, we focus on a selection model problem: a mono-exponential model versus a bi-exponential one. This is done in the biological context of living cells, where small data are available. Classical statistics are revisited to improve existing results. Some unavoidable limits are also pointed out.

  19. Case studies prepared by IOP in partnership with EPSRC and STFC | June 2013 Physics: transforming lives

    E-Print Network [OSTI]

    skills in response to the major challenges of our time, such as sustainable sources of future energy, research and application. We engage with policymakers and the general public to develop awareness scientific communications. June 2013 Physics: transforming lives I1 #12;The space industry 5 Liquid

  20. Mathematics and Equity, Past and Present, through the Lives and Work of

    E-Print Network [OSTI]

    Dahlquist, Kam D.

    Mathematics and Equity, Past and Present, through the Lives and Work of Women Mathematicians #12;Women & Mathematics Course Background · Inspired by Teri Perl's book Math Equals · Elective upper students in mathematical topics related to the work of these women · Addresses gender equity in mathematics

  1. Air Date: Week of June 16, 2006 Living on Earth's Tobin Hack reports on a

    E-Print Network [OSTI]

    Nuñez, Rafael

    Air Date: Week of June 16, 2006 Living on Earth's Tobin Hack reports on a culture where gestures on emerging science from Tobin Hack. [SCIENCE NOTE THEME] HACK: Time is a slippery concept, so people use pleased. That's this week's note on emerging science, I'm Tobin Hack. Guardian Unlimited article: How Time

  2. Five-Year Strategic Plan: 2010-2015 Improving Lives. Transforming Louisiana. Changing the World.

    E-Print Network [OSTI]

    Koppelman, David M.

    . Department of Electrical & Computer Engineering #12;Engineering is dedicated to the process of creating of life. Almost every innovation in our lives is the direct work of an engineer--roads, cars, buildings sources. with our very own way of life. Engineering is intertwined Electrical and/or computer engineering

  3. The Pollution Attack in P2P Live Video Streaming: Measurement Results and Defenses

    E-Print Network [OSTI]

    Saxena, Nitesh

    The Pollution Attack in P2P Live Video Streaming: Measurement Results and Defenses Prithula Dhungel in the recent years. In this paper, we examine the stream pollution attack, for which the attacker mixes polluted chunks into the P2P distribu- tion, degrading the quality of the rendered media at the receivers

  4. Does Growth Rate Determine the Rate of Metabolism in Shorebird Chicks Living in the Arctic?

    E-Print Network [OSTI]

    Williams, Jos. B.

    primarily of greater metabolic inten- sities of heat-generating tissues. The maximum temperature gradient500 Does Growth Rate Determine the Rate of Metabolism in Shorebird Chicks Living in the Arctic/22/2007; Electronically Published 7/13/2007 ABSTRACT We measured resting and peak metabolic rates (RMR and PMR

  5. Detecting Anomalous Sensor Events in Smart Home Data for Enhancing the Living Experience

    E-Print Network [OSTI]

    Cook, Diane J.

    Detecting Anomalous Sensor Events in Smart Home Data for Enhancing the Living Experience is on the rise and the place for smart home solutions is growing. One of the major concerns for smart home data captured from a smart home testbed. Introduction Smart homes are built by adding intelligent

  6. TorScan: Tracing Long-lived Connections and Differential Scanning Attacks

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    successor of the original Onion Routing Project. The Tor network is a low-latency anonymity network whichTorScan: Tracing Long-lived Connections and Differential Scanning Attacks Alex Biryukov, Ivan Pustogarov, Ralf-Philipp Weinmann University of Luxembourg Abstract. Tor is a widely used anonymity network

  7. innovate educate improve lives Large areas of land along the Gulf

    E-Print Network [OSTI]

    be installed with lighter equipment which has less environmental impact. Wood offers excellent work- abilityinnovate educate improve lives Large areas of land along the Gulf of Mexico have been flooded the impact of a wide array of insects, wood decay and rot. The most serious pest in this area is the Formosan

  8. INTRODUCTION Fish that live in moving water must contend with complex flows

    E-Print Network [OSTI]

    Liao, James C.

    3442 INTRODUCTION Fish that live in moving water must contend with complex flows arising from current moving past objects. Understanding how fish swim in unsteady flows has attracted attention from many disciplines, ranging from stream ecologists investigating how fish relate to habitat, to engineers

  9. Adaptive optics retinal imaging in the living Ying Geng,1,2,*

    E-Print Network [OSTI]

    Adaptive optics retinal imaging in the living mouse eye Ying Geng,1,2,* Alfredo Dubra,1,3,4 Lu Yin for Visual Science, University of Rochester, Rochester, NY 14627, USA 2 The Institute of Optics, University@corning.com Abstract: Correction of the eye's monochromatic aberrations using adaptive optics (AO) can improve

  10. LiveChat Wed, 11/16, 2 pm ET: Industrial Energy Efficiency

    Office of Energy Efficiency and Renewable Energy (EERE)

    On Wednesday, November 16, at 2:00 PM ET, join Kathleen Hogan, Deputy Assistant Secretary for Energy Efficiency, for a live discussion about the Energy Department programs how businesses are lowering their energy consumption, and how that energy savings benefits the American economy.

  11. CHEAT-PROOF COOPERATION STRATEGIES FOR WIRELESS LIVE STREAMING SOCIAL NETWORKS

    E-Print Network [OSTI]

    Liu, K. J. Ray

    in the wireless live-streaming social networks may have different types, including laptops, PDAs, cellphones and power, for instance, the laptop users would prefer higher resolution of videos and are willing to use more trans- mission power for cooperation than PDA users. They are likely to manipulate any incentive

  12. Usability is the Best Policy: Public Policy and the Lived Experience of Transport Systems in London

    E-Print Network [OSTI]

    Sasse, Angela

    Usability is the Best Policy: Public Policy and the Lived Experience of Transport Systems in London Philip Inglesant Department of Computer Science University College London Gower Street, London WC1E 6BT University College London Gower Street, London WC1E 6BT +44 (0) 20 7679 7212 A.Sasse@cs.ucl.ac.uk ABSTRACT

  13. Discovery of benzene cation in a very long-lived excited electronic state Myung Soo Kima)

    E-Print Network [OSTI]

    Kim, Myung Soo

    Discovery of benzene cation in a very long-lived excited electronic state Myung Soo Kima) and Chan, University of Suwon, Suwon 440-600, Korea Received 19 May 2000; accepted 8 September 2000 Presence of benzene chemistry. © 2000 American Institute of Physics. S0021-9606 00 01745-1 I. INTRODUCTION The benzene molecular

  14. The cost of males in non-equilibrium populations Curtis M. Lively

    E-Print Network [OSTI]

    Lively, Curt

    . Mathematical methods: The cost of males per reproductive time step was calculated as the ratio of the per-capita population. This reduction in the per-capita growth rate has been called the `cost of males' (Maynard SmithThe cost of males in non-equilibrium populations Curtis M. Lively Department of Biology, Indiana

  15. Electro-osmotic flow control for living cell analysis in microfluidic Tomasz Glawdel, Carolyn L. Ren *

    E-Print Network [OSTI]

    Le Roy, Robert J.

    Electro-osmotic flow control for living cell analysis in microfluidic PDMS chips Tomasz Glawdel April 2008 Received in revised form 20 June 2008 Available online 10 July 2008 Keywords: Electro-osmotic lines each of which consists of an integrated elec- tro-osmotic pump, a cell culture chamber

  16. Tracking chemical changes in a live cell: Biomedical Applications of SR-FTIR Spectromicroscopy

    E-Print Network [OSTI]

    Tracking chemical changes in a live cell: Biomedical Applications of SR- FTIR Spectromicroscopy Hoi Tel: 510-486-5943 Fax: 510-486-7152 E-mail: HYHolman@lbl.gov Abstract Synchrotron radiation. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal

  17. The living workplace : a conscious work environment for a small publishing company

    E-Print Network [OSTI]

    Berg, Richard Carl

    1986-01-01

    This thesis is about the oneness of working and living, and about the making of workplaces that support and encourage the idea that one's work can be an integral part of one's life. The opening position is that there is a ...

  18. Evaluating P2P Live Streaming Systems: the CNG Case Shakeel Ahmad1

    E-Print Network [OSTI]

    Evaluating P2P Live Streaming Systems: the CNG Case Shakeel Ahmad1 Christos Bouras2 Eliya Buyukkaya Network Game (CNG) project as an ex- ample to illustrate them. The evaluation of the P2P sys- tem consists streaming systems and use the CNG project as an example to illustrate them. The CNG project (http://www.cng

  19. Introduction Free-living animals need to forage for food and they may

    E-Print Network [OSTI]

    Saltzman, Wendy

    ), but when food availability is low for extended periods animals must reallocate energy to systems that need adjustments. Key words: mouse, daily energy expenditure, doubly labeled water technique, ene2013 Introduction Free-living animals need to forage for food and they may face energetic

  20. Top-down and bottom-up diversity cascades in detrital vs. living food webs

    E-Print Network [OSTI]

    Dyer, Lee

    REPORT Top-down and bottom-up diversity cascades in detrital vs. living food webs Lee A. Dyer1 for maintaining diversity in biotic communities, but the indirect (ÔcascadingÕ) effects of top-down and bottom in decomposer food webs. We measured effects of top predators and plant resources on the diversity of endophytic

  1. SQL is Dead; Long Live SQL: Lightweight Query Services for Ad Hoc Research Data

    E-Print Network [OSTI]

    Matrajt, Graciela

    SQL is Dead; Long Live SQL: Lightweight Query Services for Ad Hoc Research Data Bill Howe billhowe with ad hoc scientific Q&A. Further: · We reject the conventional wisdom that "scientists won't write SQL a production-quality database server. · We observe that simply sharing examples of SQL queries allows

  2. Insights on Psittacine Nutrition through the Study of Free-living Chicks 

    E-Print Network [OSTI]

    Cornejo, Juan

    2012-07-16

    sampled the crops from free-living chicks of scarlet macaws and red-and-green macaws from southeastern Peru, Cuban parrots from the Bahamas, lilac-crowned parrots from northwestern Mexico, and thick-billed parrots from northern Mexico. The predicted...

  3. A short-lived aeolian event during the Early Holocene in southern Norway

    E-Print Network [OSTI]

    A short-lived aeolian event during the Early Holocene in southern Norway Helena Alexanderson & Mona.alexanderson@geol.lu.se) Department of Environmental Sciences, Norwegian University of Life Sciences, Ås, Norway (mona Starmoen 1 Unit 4 The Starmoen dune field in southern Norway was formed during a single, brief phase

  4. Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis

    E-Print Network [OSTI]

    Xuan, Xiangchun "Schwann"

    Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis Saurin Biomicrofluidics 6, 034101 (2012) Fabrication of paper-based microfluidic device using printed circuit technology AIP Advances 2, 022171 (2012) Microfluidic three-dimensional hydrodynamic flow focusing for the rapid

  5. Automatic Adaptation of Fingerprint Liveness Detector to New Spoof Materials Ajita Rattani and Arun Ross

    E-Print Network [OSTI]

    Ross, Arun Abraham

    Automatic Adaptation of Fingerprint Liveness Detector to New Spoof Materials Ajita Rattani and Arun degrades upon encountering spoofs fabricated using new materials not used during the training stage detector to new spoofing materials. The aim of this work is to design a scheme for automatic adaptation

  6. five-Year strategic Plan: 2010-2015 Improving Lives. transforming Louisiana. Changing the World.

    E-Print Network [OSTI]

    Ullmer, Brygg

    devices, tools and the list goes on. Here in Louisiana, engineering is critical to our current economy. #12;#12;Engineering is dedicated to the process of creating. Engineers solve society's problems, make lives is the direct work of an engineer--roads, cars, buildings, appliances, computers, communication

  7. Implicit Siphon Control and its Role in the Liveness Enforcing Supervision of

    E-Print Network [OSTI]

    Reveliotis, Spiridon "Spyros"

    1 Implicit Siphon Control and its Role in the Liveness Enforcing Supervision of Sequential Resource (i) to correct and generalize some previously published results regarding the siphon control in PT enforcing supervision I. INTRODUCTION The role and the significance of the structural object of siphon

  8. Methods in Molecular Biology: Protocols in Confocal microscopy Chapter 17. Live imaging of green fluorescent protein

    E-Print Network [OSTI]

    Haseloff, Jim

    , an accessory protein that accepts energy from aequorin and re-emits it as green light [8] . GFP is an extremelyMethods in Molecular Biology: Protocols in Confocal microscopy Chapter 17. Live imaging of green biologists won the lottery when it was demonstrated that green fluorescent protein (GFP), a kind gift from

  9. "Sustainable" and Worth-living Integrated Development of mountainous areas in Greece and worldwide

    E-Print Network [OSTI]

    Psarrakos, Panayiotis

    "Sustainable" and Worth-living Integrated Development of mountainous areas in Greece and worldwide on initiatives of one-dimensional and mostly urban economic development. The exception to this rule has been is being discovered and its "exploitation" is being pursued, in the context of the ideology of "sustainable

  10. Live Migration of Virtual Machines Christopher Clark, Keir Fraser, Steven Hand, Jacob Gorm Hansen

    E-Print Network [OSTI]

    Simunic, Tajana

    Live Migration of Virtual Machines Christopher Clark, Keir Fraser, Steven Hand, Jacob Gorm Hansen firstname.lastname@cl.cam.ac.uk {jacobg,eric}@diku.dk Abstract Migrating operating system instances across, and low-level system maintenance. By carrying out the majority of migration while OSes con- tinue to run

  11. Live Migration of Virtual Machines Christopher Clark, Keir Fraser, Steven Hand, Jakob Gorm Hansen

    E-Print Network [OSTI]

    Hand, Steven

    Live Migration of Virtual Machines Christopher Clark, Keir Fraser, Steven Hand, Jakob Gorm Hansen firstname.lastname@cl.cam.ac.uk {jacobg,eric}@diku.dk Abstract Migrating operating system instances across, and low-level system maintenance. By carrying out the majority of migration while OSes con- tinue to run

  12. INTRODUCTION Many group-living animals communicate about the location of food

    E-Print Network [OSTI]

    Nicolis, Stamatios C.

    2337 INTRODUCTION Many group-living animals communicate about the location of food sources. Such communication is especially beneficial when food sources are ephemeral or hard to find (Sherman and Visscher and Deneubourg, 2002). Recruitment towards food sources also provides a species with the opportunity to quickly

  13. Optimal Liveness-Enforcing Control for a Class of Petri Nets Arising in Multithreaded Software

    E-Print Network [OSTI]

    Reveliotis, Spiridon "Spyros"

    1 Optimal Liveness-Enforcing Control for a Class of Petri Nets Arising in Multithreaded Software]. In this paper, we study a class of Petri nets that arises in modeling concurrent software. In this scenario hardware. This trend is making parallel programming unavoidable but concurrency bugs are making it costly

  14. Heart stopping moments with zebrafish: imaging inside the living, beating heart

    E-Print Network [OSTI]

    Greenaway, Alan

    Heart stopping moments with zebrafish: imaging inside the living, beating heart Seamless this application, enabling researchers to carry out precision "healing" studies on the moving heart by targeting of development Jonathan M. Taylor, Glasgow University d "Realtime Optical Gating for 3D Heart Imaging", Journal

  15. A C. elegans-based foam for rapid on-site detection of residual live virus.

    SciTech Connect (OSTI)

    Negrete, Oscar A.; Branda, Catherine; Hardesty, Jasper O. E. (Sandia National Laboratories, Albuquerque, NM); Tucker, Mark David (Sandia National Laboratories, Albuquerque, NM); Kaiser, Julia N. (Global Product Management, Hilden, Germany); Kozina, Carol L.; Chirica, Gabriela S.

    2012-02-01

    In the response to and recovery from a critical homeland security event involving deliberate or accidental release of biological agents, initial decontamination efforts are necessarily followed by tests for the presence of residual live virus or bacteria. Such 'clearance sampling' should be rapid and accurate, to inform decision makers as they take appropriate action to ensure the safety of the public and of operational personnel. However, the current protocol for clearance sampling is extremely time-intensive and costly, and requires significant amounts of laboratory space and capacity. Detection of residual live virus is particularly problematic and time-consuming, as it requires evaluation of replication potential within a eukaryotic host such as chicken embryos. The intention of this project was to develop a new method for clearance sampling, by leveraging Sandia's expertise in the biological and material sciences in order to create a C. elegans-based foam that could be applied directly to the entire contaminated area for quick and accurate detection of any and all residual live virus by means of a fluorescent signal. Such a novel technology for rapid, on-site detection of live virus would greatly interest the DHS, DoD, and EPA, and hold broad commercial potential, especially with regard to the transportation industry.

  16. Information technology has clearly had an impact on our daily lives. It has also altered the

    E-Print Network [OSTI]

    Information technology has clearly had an impact on our daily lives. It has also altered the global organization -- where people are sup- ported by technology, rather than the other way around. Open information-mail: gilbey@bcs.org.uk Wired for Innovation: How Information Technology is Reshaping the Economy Erik

  17. Generating audio-responsive video images in real-time for a live symphony performance 

    E-Print Network [OSTI]

    Beane, Allison Brooke

    2007-09-17

    Interactive Program……………………………….……….. IV.2.Audio Filtering………………………………………………….. IV.3.Integrating the Video and Refining the Patch………….……….. IV.3.1.Appropriate Resolution…………………………………. IV.3.2.Efficiency of Video Playback………………….……….. IV.4.Efficiently... Implementing Real-Time Filtering…………………. IV.5.Working with the Orchestra…………………………………….. IV.6.Maximizing Live Performance Variables………………………. IV.7.Recursive Testing and Patch Refinement………………………. VRESULTS AND EVALUATION …………………………………………... V.1.Evaluation...

  18. The Efficiency of Mollies, Poecilia mexicana, as Live Bait for Pole-and-Line

    E-Print Network [OSTI]

    a modem well equipped west coast live- bait vessel, J-Ann (Vergne et aI., 1978). In Hawaii, other concerning cultured baitfish spe- cies called for further field tests and for evaluation of species suitability for pole-and-line tuna fishing. This study tested the desirability of the cultured mollie

  19. Cold Climate Heat Pump Projects at Purdue University & the Living Lab

    E-Print Network [OSTI]

    Oak Ridge National Laboratory

    Cold Climate Heat Pump Projects at Purdue University & the Living Lab at the new Herrick Labs West Lafayette, Indiana 11/10/2011 1 IEA Heat Pump Program Executive Committee Meeting ASHRAE Headquarters, Atlanta, GA November 9, 2011 #12;Cold Climate Heat Pump April 1, 2010 ­ March 30, 2012 PIs

  20. "Harvesting large long-lived species on a sustainable basis is much more difficult than

    E-Print Network [OSTI]

    Manga, Michael

    as a demonstration project for several critical technologies, including a solar-powered ion motor that will provide-economicsituation,"hesays. In Europe, some half-a-million people make a living from fisheries, including ship- building and repair|www.nature.com/nature Europe dithers as Canada cuts cod fishing Asteroid probe to test technologies

  1. Addressing the Needs of Students Living in Poverty through Comprehensive School Reform

    E-Print Network [OSTI]

    Walker, Lawrence R.

    Addressing the Needs of Students Living in Poverty through Comprehensive School Reform Joseph John had 100% of their students qualifying for FRL. Nationally, Comprehensive School Reform efforts, which proposes a Full Service Community Schools model for Comprehensive School Reform, and (b) AB 448

  2. Live load effects in diaphragms of concrete slab-on-steel girder highway bridges 

    E-Print Network [OSTI]

    Saindon, Kenneth Carl

    1994-01-01

    of diaphragm member forces under vehicle live load for both the strength and fatigue limit states. These forces are shown to vary significantly among bridges with different diaphragm-deck stiffness ratios and 0 values, where 8 is a property based upon bridge...

  3. Method of freezing living cells and tissues with improved subsequent survival

    DOE Patents [OSTI]

    Senkan, Selim M. (Oak Ridge, TN); Hirsch, Gerald P. (Oak Ridge, TN)

    1980-01-01

    This invention relates to an improved method for freezing red blood cells, ther living cells, or tissues with improved subsequent survival, wherein constant-volume freezing is utilized that results in significantly improved survival compared with constant-pressure freezing; optimization is attainable through the use of different vessel geometries, cooling baths and warming baths, and sample concentrations.

  4. Aging in a very short-lived nematode Michael P. Gardnera,*, David Gemsb

    E-Print Network [OSTI]

    Gems, David

    Aging in a very short-lived nematode Michael P. Gardnera,*, David Gemsb , Mark E. Vineya a School 17 June 2004; accepted 18 June 2004 Available online 8 August 2004 Abstract Aging has been characterised in detail in relatively few animal species. Here we describe the aging process in free

  5. Health Monitoring in an Agent-Based Smart Home by Activity Prediction

    E-Print Network [OSTI]

    Cook, Diane J.

    Health Monitoring in an Agent-Based Smart Home by Activity Prediction Sajal K. Das and Diane J objective of this paper is to investigate techniques for using agent-based smart home technologies-based smart home project funded by NSF. 1 Introduction and Motivation We live in an increasingly connected

  6. Active stewardship: sustainable future

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Active stewardship: sustainable future Active stewardship: sustainable future Energy sustainability is a daunting task: How do we develop top-notch innovations with some of the...

  7. The use of fluorescent intrabodies to detect endogenous gankyrin in living cancer cells

    SciTech Connect (OSTI)

    Rinaldi, Anne-Sophie; Freund, Guillaume; Desplancq, Dominique; Sibler, Annie-Paule; Baltzinger, Mireille [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France); Rochel, Natacha [Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CNRS/INSERM/Université de Strasbourg, rue Laurent Fries, 67404 Illkirch (France); Mély, Yves; Didier, Pascal [Faculté de Pharmacie, UMR 7213, CNRS/Université de Strasbourg, route du Rhin, 67401 Illkirch (France); Weiss, Etienne, E-mail: eweiss@unistra.fr [Ecole Supérieure de Biotechnologie de Strasbourg, UMR 7242, CNRS/Université de Strasbourg, boulevard Sébastien Brant, 67412 Illkirch (France)

    2013-04-01

    Expression of antibody fragments in mammalian cells (intrabodies) is used to probe the target protein or interfere with its biological function. We previously described the in vitro characterisation of a single-chain Fv (scFv) antibody fragment (F5) isolated from an intrabody library that binds to the oncoprotein gankyrin (GK) in solution. Here, we have isolated several other scFvs that interact with GK in the presence of F5 and tested whether they allow, when fused to fluorescent proteins, to detect by FRET endogenous GK in living cells. The binding of pairs of scFvs to GK was analysed by gel filtration and the ability of each scFv to mediate nuclear import/export of GK was determined. Binding between scFv-EGFP and RFP-labelled GK in living cells was detected by fluorescence lifetime imaging microscopy (FLIM). After co-transfection of two scFvs fused to EGFP and RFP, respectively, which form a tri-molecular complex with GK in vitro, FRET signal was measured. This system allowed us to observe that GK is monomeric and distributed throughout the cytoplasm and nucleus of several cancer cell lines. Our results show that pairs of fluorescently labelled intrabodies can be monitored by FLIM–FRET microscopy and that this technique allows the detection of lowly expressed endogenous proteins in single living cells. Highlights: ? Endogenous GK in living cells was targeted with pairs of fluorescently-tagged scFvs. ? Tri-molecular complexes containing two scFvs and one molecule GK were formed. ? GK was detected using fluorescence lifetime-based FRET imaging. ? GK is monomeric and homogeneously distributed in several cancer cell lines. ? This technique may have many applications in live-cell imaging of endogenous proteins.

  8. RELATIVISTIC SUPERNOVAE HAVE SHORTER-LIVED CENTRAL ENGINES OR MORE EXTENDED PROGENITORS: THE CASE OF SN 2012ap

    SciTech Connect (OSTI)

    Margutti, R.; Milisavljevic, D.; Soderberg, A. M.; Sanders, N.; Chakraborti, S.; Kamble, A.; Drout, M.; Parrent, J.; Zauderer, A.; Guidorzi, C.; Morsony, B. J.; Ray, A.; Chomiuk, L.

    2014-12-20

    Deep, late-time X-ray observations of the relativistic, engine-driven, type Ic SN 2012ap allow us to probe the nearby environment of the explosion and reveal the unique properties of relativistic supernova explosions (SNe). We find that on a local scale of ?0.01 pc the environment was shaped directly by the evolution of the progenitor star with a pre-explosion mass-loss rate of M-dot <5×10{sup ?6} M{sub ?} yr{sup ?1}, in line with gamma-ray bursts (GRBs) and the other relativistic SN 2009bb. Like sub-energetic GRBs, SN 2012ap is characterized by a bright radio emission and evidence for mildly relativistic ejecta. However, its late-time (?t ? 20 days) X-ray emission is ?100 times fainter than the faintest sub-energetic GRB at the same epoch, with no evidence for late-time central engine activity. These results support theoretical proposals that link relativistic SNe like 2009bb and 2012ap with the weakest observed engine-driven explosions, where the jet barely fails to break out. Furthermore, our observations demonstrate that the difference between relativistic SNe and sub-energetic GRBs is intrinsic and not due to line-of-sight effects. This phenomenology can either be due to an intrinsically shorter-lived engine or to a more extended progenitor in relativistic SNe.

  9. Assisted Living

    E-Print Network [OSTI]

    Callahan, Brett

    2011-01-01

    series of leisure suits, fedoras, framed glasses, and cappedHe’s in a checkered suit, fedora, big framed glasses and

  10. Lively Infrastructure

    E-Print Network [OSTI]

    Amin, Ash

    2014-10-06

    by the city’s Urban and Housing Development Company (URBEL). In resonance with the early Lula administration’s interest in bolstering the rights of the poor and improving conditions in favelas, Vila Viva was launched in 2005 to upgrade the city’s high risk...

  11. Live Status

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of NaturalDukeWakefieldSulfateSciTechtail.Theory ofDid you notHeat PumpsTechnologies |LibraryCenterCenterLinks Berkeleylinks

  12. Light Activated Self-Propelled Colloids

    E-Print Network [OSTI]

    J. Palacci; S. Sacanna; S. -H. Kim; G. -R. Yi; D. J. Pine; P. M. Chaikin

    2014-10-27

    Light-activated self-propelled colloids are synthesized and their active motion is studied using optical microscopy. We propose a versatile route using different photoactive materials, and demonstrate a multiwavelength activation and propulsion. Thanks to the photoelectrochemical properties of two semiconductor materials (\\alpha Fe2 O3 and TiO2 ), a light with an energy higher than the bandgap triggers the reaction of decomposition of hydrogen peroxide and produces a chemical cloud around the particle. It induces a phoretic attraction with neighbouring colloids as well as an osmotic self- propulsion of the particle on the substrate. We use these mechanisms to form colloidal cargos as well as self-propelled particles where the light-activated component is embedded into a dielectric sphere. The particles are self-propelled along a direction otherwise randomized by thermal fluctuations, and exhibit a persistent random walk. For sufficient surface density, the particles spontaneously form "living crystals" which are mobile, break apart and reform. Steering the particle with an external magnetic field, we show that the formation of the dense phase results from the collisions heads-on of the particles. This effect is intrinsically non-equilibrium and a novel principle of organization for systems without detailed balance. Engineering families of particles self-propelled by different wavelength demonstrate a good understanding of both the physics and the chemistry behind the system and points to a general route for designing new families of self-propelled particles.

  13. A multivariate analysis of the energy intensity of sprawl versus compact living in the U.S. for 2003

    E-Print Network [OSTI]

    Vermont, University of

    . The methods used are based on previous studies on energy cost of living. Total energy requirement is calculated as a function of individual energy intensities of goods and services derived from economic input period. These two issues have been addressed in "energy cost of living" studies starting around 1973

  14. Modeling Computational Security in LongLived Systems # ## Ran Canetti 1,2 , Ling Cheung 2 , Dilsun Kaynar 3 ,

    E-Print Network [OSTI]

    International Association for Cryptologic Research (IACR)

    Modeling Computational Security in Long­Lived Systems # ## Ran Canetti 1,2 , Ling Cheung 2 , Dilsun Introduction Computational security in long­lived systems: Security properties of cryptographic protocols computational power. This type of security degrades progressively over the lifetime of a protocol. However, some

  15. Using the sediment archive of living dinoflagellate cysts and other protist resting stages to study temporal population dynamics

    E-Print Network [OSTI]

    Using the sediment archive of living dinoflagellate cysts and other protist resting stages to study of methodology and applications of sediment archives of living dinofla- gellates and other protists examines their potential to study ecology and evolution of unicellular eukaryotes through time. Sediment cores from Koljo

  16. Multiple sources or late injection of short-lived r-nuclides in the early solar system?

    E-Print Network [OSTI]

    Multiple sources or late injection of short-lived r-nuclides in the early solar system? N. Dauphasa abundances of short-lived r-nuclides (107 Pd, 129 I, 182 Hf, and 244 Pu) in the interstellar medium (ISM) and the observed abundances in the early solar system (ESS) conclusively showed that these nuclides cannot simply

  17. Langerhans Lab Protocols LiveFishCareDailyChecklistBRF.docx revised 10/3/2014 Page 1 of 3

    E-Print Network [OSTI]

    Langerhans, Brian

    Langerhans Lab Protocols LiveFishCareDailyChecklistBRF.docx revised 10/3/2014 Page 1 of 3 Daily Steps for Live Fish Care in BRF 221 and 223 Morning Feeding: Summary: Monday-Friday (7:00am - 10:00am disturbance to the fish after introducing the food). c. During the feeding process, make note of any dead fish

  18. LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Photography Protocol.docx revised 10/1/13 by JW Page 1 of 2

    E-Print Network [OSTI]

    Langerhans, Brian

    LLaannggeerrhhaannss LLaabb PPrroottooccoollss Live Fish Photography Protocol.docx revised 10/1/13 by JW Page 1 of 2 Live Fish Photography Protocol Set up: Stand the white board used for preserved to the white board. Position camera so that fish area fills view. Make sure auto focus is on. Make camera

  19. NMR bioreactor development for live in-situ microbial functional analysis

    SciTech Connect (OSTI)

    Majors, Paul D.; Mclean, Jeffrey S.; Scholten, Johannes C.

    2008-05-01

    A live in-situ metabolomics capability was developed for prokaryotic cultures under controlled-growth conditions. Toward this goal, a radiofrequency-transparent bioreactor was developed and integrated with a commercial wide-bore nuclear magnetic resonance (NMR) imaging spectrometer and a commercial bioreactor controller. Water suppressed 1H NMR spectroscopy was used to monitor glucose and fructose utilization and byproduct excretion by Eubacterium aggregans (an anaerobic bacterial species relevant for biofuels production) under controlled batch and continuous culture conditions. The resulting metabolite profiles (short chain organic acids and ethanol) and trends are consistent with existing knowledge of its metabolism. However, our study showed the Eubacterium aggregans produces lactate end product in significant concentrations – a result not previously reported. The advantages of live in-situ microbial metabolomics analysis and its complementariness with functional genomics / systems biology methods are discussed.

  20. Density waves in the shearing sheet IV. Interaction with a live dark halo

    E-Print Network [OSTI]

    B. Fuchs

    2004-03-01

    It is shown that if the self-gravitating shearing sheet, a model of a patch of a galactic disk, is embedded in a live dark halo, this has a strong effect on the dynamics of density waves in the sheet. I describe how the density waves and the halo interact via halo particles either on orbits in resonance with the wave or on non-resonant orbits. Contrary to expectation the presence of the halo leads to a very considerable enhancement of the amplitudes of the density waves in the shearing sheet. This effect appears to be the equivalent of the recently reported enhanced growth of bars in numerically simulated stellar disks embedded in live dark halos. Finally I discuss the transfer of linear momentum from a density wave in the sheet to the halo and show that it is mediated only by halo particles on resonant orbits.

  1. Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Holman, Hoi-Ying N.; Martin, Michael C.; McKinney, Wayne R.

    2003-01-01

    Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05-0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolismmore »in living human cells, and produces only minimal sample heating (« less

  2. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Schot, Gijs, vander

    2015-02-10

    This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.

  3. Biography and Homoeopathy in Bengal: Colonial Lives of a European Heterodoxy

    E-Print Network [OSTI]

    Das, S.

    2015-05-08

    -228 46 See for instance, James Secord (2006), ‘Scrapbook Science: Composite Carictures in Late Georgian England', in A. Shteir and B. Lightman (eds), Figuring It Out: Science, Gender, and Visual Culture, (Hanover, New Hampshire: Dartmouth College Press... , and to some extent supplanted , by a new form of biography, in which greater attention was given to complexity of character and personal motivation, to specific places and events, and to their role in shaping and explaining individual lives.’4 Introduction...

  4. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

    DOE Data Explorer [Office of Scientific and Technical Information (OSTI)]

    Schot, Gijs, vander

    This entry contains ten diffraction patterns, and reconstructions images, of individual living Cyanobium gracile cells, imaged using 517 eV X-rays from the LCLS XFEL. The Hawk software package was used for phasing. The Uppsala aerosol injector was used for sample injection, assuring very low noise levels. The cells come from various stages of the cell cycle, and were imaged in random orientations.

  5. Study of Electromagnetically Induced Transparency using long-lived Singlet States

    E-Print Network [OSTI]

    Soumya Singha Roy; T. S. Mahesh

    2011-03-17

    The long-lived singlet states are useful to study a variety of interesting quantum phenomena. In this work we study electromagnetically induced transparency using a two-qubit system. The singlet state acts as a `dark state' which does not absorb a probe radiation in the presence of a control radiation. Further we demonstrate that the simultaneous irradiation of probe and control radiations acts as a dynamical decoupling preserving the singlet state at higher correlation for longer durations.

  6. Half-lives of $?$ decay from natural nuclides and from superheavy elements

    E-Print Network [OSTI]

    Yibin Qian; Zhongzhou Ren

    2014-10-10

    Recently, experimental researches on the $\\alpha$ decay with long lifetime are one of hot topics in the contemporary nuclear physics [e.g. N. Kinoshita {\\sl et al.} (2012) and J. W. Beeman {\\sl et al.} (2012) ]. In this study, we have systematically investigated the extremely long-lived $\\alpha$-decaying nuclei within a generalized density-dependent cluster model involving the experimental nuclear charge radii. In detail, the important density distribution of daughter nuclei is deduced from the corresponding experimental charge radii, leading to an improved $\\alpha$-core potential in the quantum tunneling calculation of $\\alpha$-decay width. Besides the excellent agreement between theory and experiment, predictions on half-lives of possible candidates for natural $\\alpha$ emitters are made for future experimental detections. In addition, the recently confirmed $\\alpha$-decay chain from $^{294}$117 is well described, including the attractive long-lived $\\alpha$-decaying $^{270}$Db, i.e., a positive step towards the "island of stability" in the superheavy mass region.

  7. Effect of Long-lived Strongly Interacting Relic Particles on Big Bang Nucleosynthesis

    E-Print Network [OSTI]

    Motohiko Kusakabe; Toshitaka Kajino; Takashi Yoshida; Grant J. Mathews

    2009-06-18

    It has been suggested that relic long-lived strongly interacting massive particles (SIMPs, or $X$ particles) existed in the early universe. We study effects of such long-lived unstable SIMPs on big bang nucleosynthesis (BBN) assuming that such particles existed during the BBN epoch, but then decayed long before they could be detected. The interaction strength between an $X$ particle and a nucleon is assumed to be similar to that between nucleons. We then calculate BBN in the presence of the unstable neutral charged $X^0$ particles taking into account the capture of $X^0$ particles by nuclei to form $X$-nuclei. We also study the nuclear reactions and beta decays of $X$-nuclei. We find that SIMPs form bound states with normal nuclei during a relatively early epoch of BBN. This leads to the production of heavy elements which remain attached to them. Constraints on the abundance of $X^0$ particles during BBN are derived from observationally inferred limits on the primordial light element abundances. Particle models which predict long-lived colored particles with lifetimes longer than $\\sim$ 200 s are rejected based upon these constraints.

  8. Nanomechanical and topographical imaging of living cells by Atomic Force Microscopy with colloidal probes

    E-Print Network [OSTI]

    Luca Puricelli; Massimiliano Galluzzi; Carsten Schulte; Alessandro Podestà; Paolo Milani

    2015-02-05

    Atomic Force Microscopy (AFM) has a great potential as a tool to characterize mechanical and morphological properties of living cells; these properties have been shown to correlate with cells' fate and patho-physiological state in view of the development of novel early-diagnostic strategies. Although several reports have described experimental and technical approaches for the characterization of cell elasticity by means of AFM, a robust and commonly accepted methodology is still lacking. Here we show that micrometric spherical probes (also known as colloidal probes) are well suited for performing a combined topographic and mechanical analysis of living cells, with spatial resolution suitable for a complete and accurate mapping of cell morphological and elastic properties, and superior reliability and accuracy in the mechanical measurements with respect to conventional and widely used sharp AFM tips. We address a number of issues concerning the nanomechanical analysis, including the applicability of contact mechanical models and the impact of a constrained contact geometry on the measured elastic modulus (the finite-thickness effect). We have tested our protocol by imaging living PC12 and MDA-MB-231 cells, in order to demonstrate the importance of the correction of the finite-thickness effect and the change in cell elasticity induced by the action of a cytoskeleton-targeting drug.

  9. Ultrafast nanolaser device for detecting cancer in a single live cell.

    SciTech Connect (OSTI)

    Gourley, Paul Lee; McDonald, Anthony Eugene

    2007-11-01

    Emerging BioMicroNanotechnologies have the potential to provide accurate, realtime, high throughput screening of live tumor cells without invasive chemical reagents when coupled with ultrafast laser methods. These optically based methods are critical to advancing early detection, diagnosis, and treatment of disease. The first year goals of this project are to develop a laser-based imaging system integrated with an in- vitro, live-cell, micro-culture to study mammalian cells under controlled conditions. In the second year, the system will be used to elucidate the morphology and distribution of mitochondria in the normal cell respiration state and in the disease state for normal and disease states of the cell. In this work we designed and built an in-vitro, live-cell culture microsystem to study mammalian cells under controlled conditions of pH, temp, CO2, Ox, humidity, on engineered material surfaces. We demonstrated viability of cell culture in the microsystem by showing that cells retain healthy growth rates, exhibit normal morphology, and grow to confluence without blebbing or other adverse influences of the material surfaces. We also demonstrated the feasibility of integrating the culture microsystem with laser-imaging and performed nanolaser flow spectrocytometry to carry out analysis of the cells isolated mitochondria.

  10. KPA Activity Number

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    September 2002 Page 1 KPA Activity Number KPA Activity SEM Section SME Work Product SQSE Web Site http:cio.doe.govsqse REQUIREMENTS MANAGEMENT RM-1 The software engineering...

  11. E-cadherin junction formation involves an active kinetic nucleation process

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Biswas, Kabir H.; Hartman, Kevin L.; Yu, Cheng -han; Harrison, Oliver J.; Song, Hang; Smith, Adam W.; Huang, William Y. C.; Lin, Wan -Chen; Guo, Zhenhuan; Padmanabhan, Anup; et al

    2015-08-19

    Epithelial (E)-cadherin-mediated cell–cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit ?-catenin and exhibit remodeled cortical actin. Observations suggest thatmore »the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.« less

  12. How to Live Well Without Owning a Car: Save Money, Breathe Easier, and Get More Mileage Out of Life By Chris Balish

    E-Print Network [OSTI]

    Wong, Carleton

    2008-01-01

    Live Well Without Owning a Car: Save Money, Breathe Easier,Live Well Without Owning a Car: Save Money, Breathe Easier,mere thought of not owning a car in the United States today

  13. Bioprocessing of lignite coals using reductive microorganisms

    SciTech Connect (OSTI)

    Crawford, D.L.

    1992-03-29

    In order to convert lignite coals into liquid fuels, gases or chemical feedstock, the macromolecular structure of the coal must be broken down into low molecular weight fractions prior to further modification. Our research focused on this aspect of coal bioprocessing. We isolated, characterized and studied the lignite coal-depolymerizing organisms Streptomyces viridosporus T7A, Pseudomonas sp. DLC-62, unidentified bacterial strain DLC-BB2 and Gram-positive Bacillus megaterium strain DLC-21. In this research we showed that these bacteria are able to solubilize and depolymerize lignite coals using a combination of biological mechanisms including the excretion of coal solublizing basic chemical metabolites and extracellular coal depolymerizing enzymes.

  14. Microorganism genomics, compositions and methods related thereto

    DOE Patents [OSTI]

    Handelsman, Jo (Madison, WI); Goodman, Robert M. (Madison, WI); Rondon, Michelle R. (Madison, WI)

    2001-01-01

    The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

  15. Biodegradation of Triclosan by Aerobic Microorganisms 

    E-Print Network [OSTI]

    Lee, Do Gyun

    2012-10-19

    Triclosan, a synthetic antimicrobial agent, is an emerging environmental contaminant. Due to incomplete removal of triclosan by wastewater treatment plants, treated wastewater is one major source of environmental triclosan. Biodegradation...

  16. Bacteriocins of Aquatic Microorganisms and Their Potential

    E-Print Network [OSTI]

    Riley, Margaret

    stage (Gillor et al., 2008). The use of bacteriocins in probiotic applications, as preservatives, and promise for use in the creation of probiotic strains for the seafood industry. 2. Bacteriocin basics

  17. Pentanol isomer synthesis in engineered microorganisms

    E-Print Network [OSTI]

    Cann, Anthony F.; Liao, James C.

    2010-01-01

    For example, current biodiesel production relies mostly onenzymes for in vivo biodiesel production (Kalscheuer et al.292 Gerpen JV (2005) Biodiesel processing and production.

  18. Lasers in our lives 50 years of impact The information in this brochure is just a sample of the significant social and

    E-Print Network [OSTI]

    of the significant social and economic impact that lasers have had on our lives over the past 50 years. BecauseLasers in our lives 50 years of impact #12;The information in this brochure is just a sample Services Lasers in our lives 50 years of impact #12;Introduction What are lasers? Light Amplification

  19. Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells

    E-Print Network [OSTI]

    Cermak, Nathan

    We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell’s buoyant ...

  20. The Effects of Mandatory Country-of-Origin Labeling on Canadian/U.S. Live Hog and Feeder Pig Trade 

    E-Print Network [OSTI]

    Thevenaz, Shad Arthur Michel

    2012-02-14

    The final implementation of Mandatory Country-of-Origin Labeling has caused some U.S. packing plants and finishing operations to discontinue using Canadian live hogs and feeder pigs in their operations thereby reducing trade. Using a system...

  1. Negotiating Worlds, Managing Subjectivities, and Redefining Selves: The Lived Experiences of African American Undergraduate Females at Predominately White Institutions 

    E-Print Network [OSTI]

    Allen, Ayana Ma-El

    2011-02-22

    A narrative analysis of the lived experiences of seven undergraduate African American females at Predominately White Institutions (PWIs) is presented in this study. The purpose of the study was to explore the ways the ...

  2. THE HEALTH BENEFITS OF CAFFEINE Everything you need to know to think faster, exercise harder, and live longer

    E-Print Network [OSTI]

    Lockery, Shawn

    to let you know it's time to turn off Conan and recharge your batteries. Adenosine accomplishes, and live longer By Lauren Russell Griffin, Photo Illustrations by Eddie Guy By now you'd think scientists

  3. How Ethno-Religious Identity Influences the Living Conditions of Hazara and Pashtun Refugees in Peshawar, Pakistan

    E-Print Network [OSTI]

    Punjani, Shahid

    This paper examines how living conditions and settlement patterns differ between two ethnic groups belonging to the Afghan refugee population residing in Peshawar, Pakistan. Evidence from Peshawar suggests that the Pashtun, ...

  4. Quantum Dot Targeting with Lipoic Acid Ligase and HaloTag for Single-Molecule Imaging on Living Cells

    E-Print Network [OSTI]

    Liu, Daniel S.

    We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a ...

  5. Collective spaces : a study in the conversion of storage to living spaces in City of Industry, California

    E-Print Network [OSTI]

    Su, Tony H. (Tony Hsuan Ching)

    2005-01-01

    A research and design study was instigated to rethink the phenomenon of storage in relation to contemporary living spheres. Although few historical traces of personal storage remain, the study of the evolution of commercial ...

  6. Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging

    E-Print Network [OSTI]

    White, Katharine Alice

    2012-01-01

    Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. ...

  7. Influence of Diet on Performance Parameters, Intestinal Lesion Development, and Oocyst Cycling in Live Oocyst Vaccinated Replacement Broiler Breeders 

    E-Print Network [OSTI]

    Oden, Leslee Ann

    2010-10-12

    breeders of two different genetic lines vaccinated with a live coccidiosis vaccine. Dietary formulations were based on either breeder specific recommendations or formulations of a broiler integrator. On day 28, males of each genetic line were added...

  8. hen the first live eastern oysters came to the Bay Area by train in the late 1800s,Victorian-

    E-Print Network [OSTI]

    and their love of trendy food, would-be oyster farmers followed, hoping to raise their imported shellfish-tank vacuum to see what's living at the site-- part of the Boyer lab's regular monitoring of the reefs

  9. Energy Conservation and Comfort of Heat Pump Desiccant Air Conditioning System in Actual Living Space in Summer

    E-Print Network [OSTI]

    Miyashita, Yasushi

    Energy Conservation and Comfort of Heat Pump Desiccant Air Conditioning System in Actual Living and total heat exchanger in terms of both energy conservation and thermal comfort in summer. 1. COP

  10. While living in Phoenix, Arizona, several years ago, it was always a pleasure to take the four-

    E-Print Network [OSTI]

    Gorelick, Root

    While living in Phoenix, Arizona, several years ago, it was always a pleasure to take the four minutes from your car. Because almost no sane person visits Phoenix in summer, I never made

  11. Moving past: probing the agency and affect of recordkeeping in individual and community lives in post-conflict Croatia

    E-Print Network [OSTI]

    Gilliland, AJ

    2014-01-01

    settlers in post- conflict Croatia, Intl J Hum Rights 17:Northern Ireland and Croatia, Fletcher F. World Aff 16:29-50Lives in Post-conflict Croatia --Manuscript Draft--

  12. Understanding the Triaging and Fixing Processes of Long Lived Bugs Ripon K. Saha, Sarfraz Khurshid, Dewayne E. Perry

    E-Print Network [OSTI]

    Perry, Dewayne E.

    Understanding the Triaging and Fixing Processes of Long Lived Bugs Ripon K. Saha, Sarfraz Khurshid- velopers/managers triage bugs carefully and schedule Email addresses: ripon@utexas.edu (Ripon K. Saha

  13. Developing new optical imaging techniques for single particle and molecule tracking in live cells

    SciTech Connect (OSTI)

    Sun, Wei

    2010-12-15

    Differential interference contrast (DIC) microscopy is a far-field as well as wide-field optical imaging technique. Since it is non-invasive and requires no sample staining, DIC microscopy is suitable for tracking the motion of target molecules in live cells without interfering their functions. In addition, high numerical aperture objectives and condensers can be used in DIC microscopy. The depth of focus of DIC is shallow, which gives DIC much better optical sectioning ability than those of phase contrast and dark field microscopies. In this work, DIC was utilized to study dynamic biological processes including endocytosis and intracellular transport in live cells. The suitability of DIC microscopy for single particle tracking in live cells was first demonstrated by using DIC to monitor the entire endocytosis process of one mesoporous silica nanoparticle (MSN) into a live mammalian cell. By taking advantage of the optical sectioning ability of DIC, we recorded the depth profile of the MSN during the endocytosis process. The shape change around the nanoparticle due to the formation of a vesicle was also captured. DIC microscopy was further modified that the sample can be illuminated and imaged at two wavelengths simultaneously. By using the new technique, noble metal nanoparticles with different shapes and sizes were selectively imaged. Among all the examined metal nanoparticles, gold nanoparticles in rod shapes were found to be especially useful. Due to their anisotropic optical properties, gold nanorods showed as diffraction-limited spots with disproportionate bright and dark parts that are strongly dependent on their orientation in the 3D space. Gold nanorods were developed as orientation nanoprobes and were successfully used to report the self-rotation of gliding microtubules on kinesin coated substrates. Gold nanorods were further used to study the rotational motions of cargoes during the endocytosis and intracellular transport processes in live mammalian cells. New rotational information was obtained: (1) during endocytosis, cargoes lost their rotation freedom at the late stage of internalization; (2) cargoes performed train-like motion when they were transported along the microtubule network by motor proteins inside live cells; (3) During the pause stage of fast axonal transport, cargoes were still bound to the microtubule tracks by motor proteins. Total internal reflection fluorescence microscopy (TIRFM) is another non-invasive and far-field optical imaging technique. Because of its near-field illumination mechanism, TIRFM has better axial resolution than epi-fluorescence microscopy and confocal microscopy. In this work, an auto-calibrated, prism type, angle-scanning TIRFM instrument was built. The incident angle can range from subcritical angles to nearly 90{sup o}, with an angle interval less than 0.2{sup o}. The angle precision of the new instrument was demonstrated through the finding of the surface plasmon resonance (SPR) angle of metal film coated glass slide. The new instrument improved significantly the precision in determining the axial position. As a result, the best obtained axial resolution was {approx} 8 nm, which is better than current existing instruments similar in function. The instrument was further modified to function as a pseudo TIRF microscope. The illumination depth can be controlled by changing the incident angle of the excitation laser beam or adjusting the horizontal position of the illumination laser spot on the prism top surface. With the new technique, i.e., variable-illumination-depth pseudo TIRF microscopy, the whole cell body from bottom to top was scanned.

  14. Evaluation of a modified-live, gene deletion mutant pseudorabies virus vaccine for field use in swine 

    E-Print Network [OSTI]

    Lawhorn, Donald Bruce

    1989-01-01

    EVALUATION OF A MODIFIED-LIVE, GENE DELETION MUTANT PSEUDORABIES VIRUS VACCINE FOR FIELD USE IN SWINE A Thesis by DONALD BRUCE LAWHORN Submitted to the Office of Graduate Studies of Texas ASM University in partial fulfillment... of the requirements for the degree of MASTER OF SCIENCE August 1989 Major Subject: Veterinary Microbiblogy EVALUATION OF A MODIFIED-LIVE, GENE DELETION MUTANT PSEUDORABIES VIRUS VACCINE FOR FIELD USE IN SWINE A Thesis by DONALD BRUCE LAWHORN Approved...

  15. "Table HC1.2.3 Living Space Characteristics by Average Floorspace--"

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Informationmonthly gasoline price to fall to $3.43U.S.longec 188 U.S.1 HomeRegional3 Housing Unit3 Living

  16. "Table HC12.2 Living Space Characteristics by Midwest Census Region, 2005"

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Informationmonthly gasoline price to fall to $3.43U.S.longec 188 U.S.1 HomeRegional3 Housing242 Living

  17. Effects of LWR coolant environments on fatigue lives of austenitic stainless steels.

    SciTech Connect (OSTI)

    Chopra, O. K.

    1998-01-13

    Fatigue tests have been conducted on Types 304 and 316NG stainless steels to evaluate the effects of various material and loading variables, e.g., steel type, strain rate, dissolved oxygen (DO) in water, and strain range, on the fatigue lives of these steels. The results confirm significant decreases in fatigue life in water. Unlike the situation with ferritic steels, environmental effects on Types 304 and 316NG stainless steel are more pronounced in low-DO than in high-DO water. Experimental results have been compared with estimates of fatigue life based on a statistical model. The formation and growth of fatigue cracks in air and water environments are discussed.

  18. Search for long-lived isomeric states in neutron-deficient thorium isotopes

    E-Print Network [OSTI]

    J. Lachner; I. Dillmann; T. Faestermann; G. Korschinek; M. Poutivtsev; G. Rugel

    2009-07-01

    The discovery of naturally occurring long-lived isomeric states (t_1/2 > 10^8 yr) in the neutron-deficient isotopes 211,213,217,218Th [A. Marinov et al., Phys. Rev. C 76, 021303(R) (2007)] was reexamined using accelerator mass spectrometry (AMS). Because AMS does not suffer from molecular isobaric background in the detection system, it is an extremely sensitive technique. Despite our up to two orders of magnitude higher sensitivity we cannot confirm the discoveries of neutron-deficient thorium isotopes and provide upper limits for their abundances.

  19. Installation of 2 7/8-in. coiled-tubing tailpipes in live gas wells

    SciTech Connect (OSTI)

    Campbell, J.A.; Bayes, K.P.

    1994-05-01

    This paper describes a technique for installing 2 7/8-in. coiled tubing as tailpipe extensions below existing production packers in live gas wells. It also covers the use of coiled tubing as a way to complete wells. Large savings in rig time and deferred production have been realized with this technique. Fluid losses to the formation do not occur, and no expensive rig time is needed to kill or clean up the wells, as required for conventional workovers below existing production packers. This technique is particularly applicable in depleted reservoirs that could be impaired by traditional workover methods.

  20. System, device, and methods for real-time screening of live cells, biomarkers, and chemical signatures

    DOE Patents [OSTI]

    Sundaram, S Kamakshi [Richland, WA; Riley, Brian J [West Richland, WA; Weber, Thomas J [Richland, WA; Sacksteder, Colette A [West Richland, WA; Addleman, R Shane [Benton City, WA

    2011-06-07

    An ATR-FTIR device and system are described that defect live-cell responses to stimuli and perturbations in real-time. The system and device can monitor perturbations resulting from exposures to various physical, chemical, and biological materials in real-time, as well as those sustained over a long period of time, including those associated with stimuli having unknown modes-of-action (e.g. nanoparticles). The device and system can also be used to identify specific chemical species or substances that profile cellular responses to these perturbations.

  1. Long-lived stop at the LHC with or without R-parity

    SciTech Connect (OSTI)

    Covi, L.; Dradi, F., E-mail: laura.covi@theorie.physik.uni-goettingen.de, E-mail: federico.dradi@theorie.physik.uni-goettingen.de [Institut für Theoretische Physik, Friedrich-Hund-Platz 1, 37077 Göttingen (Germany)

    2014-10-01

    We consider scenarios of gravitino LSP and DM with stop NLSP both within R-parity conserving and R-parity violating supersymmetry (RPC and RPV SUSY, respectively). We discuss cosmological bounds from Big Bang Nucleosynthesis (BBN) and the gravitino abundance and then concentrate on the signals of long-lived stops at the LHC as displaced vertices or metastable particles. Finally we discuss how to distinguish R-parity conserving and R-parity breaking stop decays if they happen within the detector and how to suppress SM backgrounds.

  2. The Woodlake cooperative community: a new deal experiment in rural living for the unemployed 

    E-Print Network [OSTI]

    Booker, James Terry

    1976-01-01

    'ID OF TOWN AND COUI4", RY V. PROBLEMS AND ADJUSTFIENT VI. THE WOODLAKE EXPERI!'IENT: ABANDONED B IBL10BRAPHY VITA. V1 U11 VI41 1B AC 10( 2 12B LIST OF TABLES Table 1 . Budget for Family of Six for One Year. Page Clothes Budget for Family...Ie and content by: 4a P ~&+@+ December 1976 APB;RACT The Noodlake Cocperat', ve Community: R New Deal Experiment in Rural Living for the Unemployed IDeceml:er 1976) James Terry Booker, B. S. Ed. . Abilene Chri'tian Un'iversity Chairu!an of Advisory...

  3. Table HC1.2.2 Living Space Characteristics by Average Floorspace

    U.S. Energy Information Administration (EIA) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page| Open Energy Informationmonthly gasoline price toStocksU.S. shale gas plays:0a. Housing Unit5a. Housing12 Living

  4. Utility Rates API Version 2 is Live! | OpenEI Community

    Open Energy Info (EERE)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on QA:QA J-E-1 SECTION J APPENDIX ECoop IncIowa (Utility Company) JumpGTZUtility Rates API Version 2 is Live! Home >

  5. LIVE Q&A TODAY: Answering Your Wind Energy Questions | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACTThousand CubicResource and JobLCLS Operating3ledp/ The4, LINEGasLIVE

  6. WATCH LIVE: Talking the Higgs Boson with CERN | Department of Energy

    Office of Environmental Management (EM)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantity of Natural GasAdjustmentsShirley Ann Jackson About1996HowFOAShowingFuel Efficiency &Report- April 2012 |VoteWATCH LIVE:

  7. #AskEnergy: Live Twitter Q&A on Solar Energy | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative FuelsofProgram:Y-12 Beta-3 Racetracks Y-12 Beta-3of thePolicies Act of 1978Live

  8. Night of the Living Trash: Bringing Your Waste Back to Life | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home Page on Delicious Rank EERE: Alternative Fuelsof EnergyApril 2014Department ofWind Career MapPower Systems » NextLive

  9. LiveChat Thurs, 10/20, 2pm ET: Clean Tech Markets | Department of Energy

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Homesum_a_epg0_fpd_mmcf_m.xls" ,"Available from WebQuantityBonneville Power Administration would likeUniverseIMPACTThousand CubicResourcelogo and-E CChinaC L S C O N C2 ArnoldLiveChat

  10. Experimental neutronics tests for a neutron activation system for the European ITER TBM

    SciTech Connect (OSTI)

    Klix, A.; Fischer, U. [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany); Gehre, D. [Technical University of Dresden, IKTP, Zellescher Weg 19, 01062 Dresden (Germany); Kleizer, G. [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Budapest University of Technology and Economics, M?egyetem rkp. 3-9. H-1111 Budapest (Hungary); Raj, P. [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Université Paris-Sud, 15 rue Georges Clemenceau, F-91405 Paris (France); Rovni, I. [Budapest University of Technology and Economics, M?egyetem rkp. 3-9. H-1111 Budapest (Hungary); Ruecker, Tom [Karlsruhe Institute of Technology (KIT), INR, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and University of Applied Sciences Zittau-Goerlitz, Theodor-Körner-Allee 16, D-02754 Zittau (Germany)

    2014-08-21

    We are investigating methods for neutron flux measurement in the ITER TBM. In particular we have tested sets of activation materials leading to induced gamma activities with short half-lives of the order of tens of seconds up to minutes and standard activation materials. Packages of activation foils have been irradiated with the intense neutron generator of Technical University of Dresden in a pure DT neutron field as well as in a neutronics mock-up of the European ITER HCLL TBM. An important aim was to check whether the gamma activity induced in the activation foils in these packages could be measured simultaneously. It was indeed possible to identify gamma lines of interest in gamma-ray measurements immediately after extraction from the irradiation.

  11. Activated carbon aerogels

    SciTech Connect (OSTI)

    Hanzawa, Y.; Kaneko, K. [Chiba Univ. (Japan)] [Chiba Univ. (Japan); Pekala, R.W. [Lawrence Livermore National Lab., CA (United States)] [Lawrence Livermore National Lab., CA (United States); Dresselhaus, M.S. [Massachusetts Inst. of Technology, Cambridge, MA (United States)] [Massachusetts Inst. of Technology, Cambridge, MA (United States)

    1996-12-25

    Activated carbon aerogels were obtained from the CO{sub 2} activation of the carbon aerogels. The adsorption isotherms of nitrogen on activated carbon aerogels at 77 K were measured and analyzed by the high-resolution {alpha}{sub s} plot to evaluate their porosities. The {alpha}{sub s} plot showed an upward deviation from linearity below {alpha}{sub s} = 0.5, suggesting that the presence of micropores becomes more predominant with the extent of the activation. Activation increased noticeably the pore volume and the surface area (the maximum value: 2600 m{sup 2}.g{sup -1}) without change of the basic network structure of primary particles. Activated carbon aerogels had a bimodal pore size distribution of uniform micropores and mesopores. 16 refs., 2 figs., 1 tab.

  12. Search for long-lived gravitational-wave transients coincident with long gamma-ray bursts

    E-Print Network [OSTI]

    The LIGO Scientific Collaboration; the Virgo Collaboration; J. Aasi; J. Abadie; B. P. Abbott; R. Abbott; T. Abbott; M. R. Abernathy; T. Accadia; F. Acernese; C. Adams; T. Adams; R. X. Adhikari; C. Affeldt; M. Agathos; N. Aggarwal; O. D. Aguiar; P. Ajith; B. Allen; A. Allocca; E. Amador Ceron; D. Amariutei; R. A. Anderson; S. B. Anderson; W. G. Anderson; K. Arai; M. C. Araya; C. Arceneaux; J. Areeda; S. Ast; S. M. Aston; P. Astone; P. Aufmuth; C. Aulbert; L. Austin; B. E. Aylott; S. Babak; P. T. Baker; G. Ballardin; S. W. Ballmer; J. C. Barayoga; D. Barker; S. H. Barnum; F. Barone; B. Barr; L. Barsotti; M. Barsuglia; M. A. Barton; I. Bartos; R. Bassiri; A. Basti; J. Batch; J. Bauchrowitz; Th. S. Bauer; M. Bebronne; B. Behnke; M. Bejger; M. G. Beker; A. S. Bell; C. Bell; I. Belopolski; G. Bergmann; J. M. Berliner; D. Bersanetti; A. Bertolini; D. Bessis; J. Betzwieser; P. T. Beyersdorf; T. Bhadbhade; I. A. Bilenko; G. Billingsley; J. Birch; M. Bitossi; M. A. Bizouard; E. Black; J. K. Blackburn; L. Blackburn; D. Blair; M. Blom; O. Bock; T. P. Bodiya; M. Boer; C. Bogan; C. Bond; F. Bondu; L. Bonelli; R. Bonnand; R. Bork; M. Born; V. Boschi; S. Bose; L. Bosi; J. Bowers; C. Bradaschia; P. R. Brady; V. B. Braginsky; M. Branchesi; C. A. Brannen; J. E. Brau; J. Breyer; T. Briant; D. O. Bridges; A. Brillet; M. Brinkmann; V. Brisson; M. Britzger; A. F. Brooks; D. A. Brown; D. D. Brown; F. Br; T. Bulik; H. J. Bulten; A. Buonanno; D. Buskulic; C. Buy; R. L. Byer; L. Cadonati; G. Cagnoli; J. Calderon Bustillo; E. Calloni; J. B. Camp; P. Campsie; K. C. Cannon; B. Canuel; J. Cao; C. D. Capano; F. Carbognani; L. Carbone; S. Caride; A. Castiglia; S. Caudill; M. Cavagli; F. Cavalier; R. Cavalieri; G. Cella; C. Cepeda; E. Cesarini; R. Chakraborty; T. Chalermsongsak; S. Chao; P. Charlton; E. Chassande-Mottin; X. Chen; Y. Chen; A. Chincarini; A. Chiummo; H. S. Cho; J. Chow; N. Christensen; Q. Chu; S. S. Y. Chua; S. Chung; G. Ciani; F. Clara; D. E. Clark; J. A. Clark; F. Cleva; E. Coccia; P. -F. Cohadon; A. Colla; M. Colombini; M. Constancio Jr.; A. Conte; R. Conte; D. Cook; T. R. Corbitt; M. Cordier; N. Cornish; A. Corsi; C. A. Costa; M. W. Coughlin; J. -P. Coulon; S. Countryman; P. Couvares; D. M. Coward; M. Cowart; D. C. Coyne; K. Craig; J. D. E. Creighton; T. D. Creighton; S. G. Crowder; A. Cumming; L. Cunningham; E. Cuoco; K. Dahl; T. Dal Canton; M. Damjanic; S. L. Danilishin; S. D'Antonio; K. Danzmann; V. Dattilo; B. Daudert; H. Daveloza; M. Davier; G. S. Davies; E. J. Daw; R. Day; T. Dayanga; R. De Rosa; G. Debreczeni; J. Degallaix; W. Del Pozzo; E. Deleeuw; S. Deleglise; T. Denker; T. Dent; H. Dereli; V. Dergachev; R. DeRosa; R. DeSalvo; S. Dhurandhar; L. Di Fiore; A. Di Lieto; I. Di Palma; A. Di Virgilio; M. Diaz; A. Dietz; K. Dmitry; F. Donovan; K. L. Dooley; S. Doravari; M. Drago; R. W. P. Drever; J. C. Driggers; Z. Du; J. -C. Dumas; S. Dwyer; T. Eberle; M. Edwards; A. Effler; P. Ehrens; J. Eichholz; S. S. Eikenberry; G. EndrH; R. Essick; T. Etzel; K. Evans; M. Evans; T. Evans; M. Factourovich; V. Fafone; S. Fairhurst; Q. Fang; S. Farinon; B. Farr; W. Farr; M. Favata; D. Fazi; H. Fehrmann; D. Feldbaum; I. Ferrante; F. Ferrini; F. Fidecaro; L. S. Finn; I. Fiori; R. Fisher; R. Flaminio; E. Foley; S. Foley; E. Forsi; N. Fotopoulos; J. -D. Fournier; S. Franco; S. Frasca; F. Frasconi; M. Frede; M. Frei; Z. Frei; A. Freise; R. Frey; T. T. Fricke; P. Fritschel; V. V. Frolov; M. -K. Fujimoto; P. Fulda; M. Fyffe; J. Gair; L. Gammaitoni; J. Garcia; F. Garufi; N. Gehrels; G. Gemme; E. Genin; A. Gennai; L. Gergely; S. Ghosh; J. A. Giaime; S. Giampanis; K. D. Giardina; A. Giazotto; S. Gil-Casanova; C. Gill; J. Gleason; E. Goetz; R. Goetz; L. Gondan; G. Gonzalez; N. Gordon; M. L. Gorodetsky; S. Gossan; S. Go; R. Gouaty; C. Graef; P. B. Graff; M. Granata; A. Grant; S. Gras; C. Gray; R. J. S. Greenhalgh; A. M. Gretarsson; C. Griffo; P. Groot; H. Grote; K. Grover; S. Grunewald; G. M. Guidi; C. Guido; K. E. Gushwa; E. K. Gustafson; R. Gustafson; B. Hall; E. Hall; D. Hammer; G. Hammond; M. Hanke; J. Hanks; C. Hanna; J. Hanson; J. Harms; G. M. Harry; I. W. Harry; E. D. Harstad; M. T. Hartman; K. Haughian; K. Hayama; J. Heefner; A. Heidmann; M. Heintze; H. Heitmann; P. Hello; G. Hemming; M. Hendry; I. S. Heng; A. W. Heptonstall; M. Heurs; S. Hild; D. Hoak; K. A. Hodge; K. Holt; M. Holtrop; T. Hong; S. Hooper; T. Horrom; D. J. Hosken; J. Hough; E. J. Howell; Y. Hu; Z. Hua; V. Huang; E. A. Huerta; B. Hughey; S. Husa; S. H. Huttner; M. Huynh; T. Huynh-Dinh; J. Iafrate; D. R. Ingram; R. Inta; T. Isogai; A. Ivanov; B. R. Iyer; K. Izumi; M. Jacobson; E. James; H. Jang; Y. J. Jang; P. Jaranowski; F. Jimenez-Forteza; W. W. Johnson; D. Jones; D. I. Jones; R. Jones; R. J. G. Jonker; L. Ju; Haris K; P. Kalmus; V. Kalogera; S. Kandhasamy; G. Kang; J. B. Kanner; M. Kasprzack; R. Kasturi; E. Katsavounidis; W. Katzman; H. Kaufer; K. Kaufman

    2013-12-09

    Long gamma-ray bursts (GRBs) have been linked to extreme core-collapse supernovae from massive stars. Gravitational waves (GW) offer a probe of the physics behind long GRBs. We investigate models of long-lived (~10-1000s) GW emission associated with the accretion disk of a collapsed star or with its protoneutron star remnant. Using data from LIGO's fifth science run, and GRB triggers from the swift experiment, we perform a search for unmodeled long-lived GW transients. Finding no evidence of GW emission, we place 90% confidence level upper limits on the GW fluence at Earth from long GRBs for three waveforms inspired by a model of GWs from accretion disk instabilities. These limits range from F<3.5 ergs cm^-2 to $F<1200 ergs cm^-2, depending on the GRB and on the model, allowing us to probe optimistic scenarios of GW production out to distances as far as ~33 Mpc. Advanced detectors are expected to achieve strain sensitivities 10x better than initial LIGO, potentially allowing us to probe the engines of the nearest long GRBs.

  13. Limit on the mass of a long-lived or stable gluino

    E-Print Network [OSTI]

    G. R. Farrar; R. Mackeprang; D. Milstead; J. P. Roberts

    2011-01-23

    We reinterpret the generic CDF charged massive particle limit to obtain a limit on the mass of a stable or long-lived gluino. Various sources of uncertainty are examined. The $R$-hadron spectrum and scattering cross sections are modeled based on known low-energy hadron physics and the resultant uncertainties are quantified and found to be small compared to uncertainties from the scale dependence of the NLO pQCD production cross sections. The largest uncertainty in the limit comes from the unknown squark mass: when the squark -- gluino mass splitting is small, we obtain a gluino mass limit of 407 GeV, while in the limit of heavy squarks the gluino mass limit is 397 GeV. For arbitrary (degenerate) squark masses, we obtain a lower limit of 322 GeV on the gluino mass. These limits apply for any gluino lifetime longer than $\\sim 30$ ns, and are the most stringent limits for such a long-lived or stable gluino.

  14. Short-lived pollutants in the Arctic: their climate impact and possible mitigation strategies

    SciTech Connect (OSTI)

    Menon, Surabi; Quinn, P.K.; Bates, T.S.; Baum, E.; Doubleday, N.; Fiore, A.M.; Flanner, M.; Fridlind, A.; Garrett, T.J.; Koch, D.; Menon, S.; Shindell, D.; Stohl, A.; Warren, S.G.

    2007-09-24

    Several short-lived pollutants known to impact Arctic climate may be contributing to the accelerated rates of warming observed in this region relative to the global annually averaged temperature increase. Here, we present a summary of the short-lived pollutants that impact Arctic climate including methane, tropospheric ozone, and tropospheric aerosols. For each pollutant, we provide a description of the major sources and the mechanism of forcing. We also provide the first seasonally averaged forcing and corresponding temperature response estimates focused specifically on the Arctic. The calculations indicate that the forcings due to black carbon, methane, and tropospheric ozone lead to a positive surface temperature response indicating the need to reduce emissions of these species within and outside the Arctic. Additional aerosol species may also lead to surface warming if the aerosol is coincident with thin, low lying clouds. We suggest strategies for reducing the warming based on current knowledge and discuss directions for future research to address the large remaining uncertainties.

  15. Method for detection of long-lived radioisotopes in small biochemical samples

    DOE Patents [OSTI]

    Turteltaub, Kenneth W. (Livermore, CA); Vogel, John S. (Union City, CA); Felton, James S. (Danville, CA); Gledhill, Barton L. (Alamo, CA); Davis, Jay C. (Livermore, CA)

    1994-01-01

    Disclosed is a method for detection of long-lived radioisotopes in small bio-chemical samples, comprising: a. selecting a biological host in which radioisotopes are present in concentrations equal to or less than those in the ambient biosphere, b. preparing a long-lived radioisotope labeled reactive chemical specie, c. administering said chemical specie to said biologist host in doses sufficiently low to avoid significant overt damage to the biological system thereof, d. allowing a period of time to elapse sufficient for dissemination and interaction of said chemical specie with said host throughout said biological system of said host, e. isolating a reacted fraction of the biological substance from said host in a manner sufficient to avoid contamination of said substance from extraneous sources, f. converting said fraction of biological substance by suitable means to a material which efficiently produces charged ions in at least one of several possible ion sources without introduction of significant isotopic fractionation, and, g. measuring the radioisotope concentration in said material by means of direct isotopic counting.

  16. Trapping and Assembly of Living Colloids at Water/Water Interfaces

    E-Print Network [OSTI]

    Sarah D. Hann; Mark Goulian; Daeyeon Lee; Kathleen J. Stebe

    2014-12-11

    We study the assembly of colloids in a two phase water-water system that provides an environment that can sustain bacteria, providing a new structure with rich potential to confine and structure living colloids. The water-water system, formed via phase separation of a casein and xanthan mixture, forms a 3-D structure of coexisting casein-rich and xanthan-rich phases. Fluorescent labelling and confocal microscopy reveal the attachment of these living colloids, including Escherichia coli and Pseudomonas aeruginosa, at the interface between the two phases. Inert colloids also become trapped at the interfaces, suggesting that the observed attachment can be attributed to capillarity. Over time, these structures coarsen and eventually degrade, illustrating the dynamic nature of these systems. This system lays the foundation for future studies of the interplay of physicochemical properties of the fluid interfaces and bulk phases and microbial responses they provoke to induce complex spatial organization, to study species which occupy distinct niches, and to optimize efficient microbial cross-feeding or protection from competitors.

  17. The Irradiation Origin of Beryllium Radioisotopes and Other Short-lived Radionuclides

    E-Print Network [OSTI]

    Matthieu Gounelle; Frank H. Shu; Hsien Shang; A. E. Glassgold; K. E. Rehm; Typhoon Lee

    2005-12-21

    Two explanations exist for the short-lived radionuclides present in the solar system when the CAIs first formed. They originated either from the ejecta of a supernova or by the in situ irradiation of nebular dust by energetic particles. With a half-life of only 53 days, Beryllium-7 is then the key discriminant, since it can be made only by irradiation. We calculate the yield of Be-7. Within model uncertainties associated mainly with nuclear cross sections, we obtain agreement with the experimental value. Moreover, if Be-7 and Be-10 have the same origin, the irradiation time must be short. The x-wind model provides a natural astrophysical setting that gives the requisite conditions. The decoupling of the Al-26 and Be-10 observed in some rare CAIs receives a quantitative explanation when rare gradual events are considered. Finally, we show that the presence of supernova-produced Fe-60 in the solar accretion disk does not necessarily mean that other short-lived radionuclides have a stellar origin.

  18. Activated recombinant adenovirus proteinases

    DOE Patents [OSTI]

    Anderson, C.W.; Mangel, W.F.

    1999-08-10

    This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described. 29 figs.

  19. Energy Conservation Standards Activities

    Broader source: Energy.gov (indexed) [DOE]

    Congress August 2014 United States Department of Energy Washington, DC 20585 Energy Conservation Standards Activities Report to Congress | Page i Message from the Assistant...

  20. Low Specific Activity (LSA)

    Broader source: Energy.gov [DOE]

    This scenario provides the planning instructions, guidance, and evaluation forms necessary to conduct an exercise involving a highway shipment of Low Specific Activity (LSA) material.  This...