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Sample records for mi nc ny

  1. NC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NC π 0 Production in the MiniBooNE Antineutrino Data V. T. Nguyen, for the MiniBooNE Collaboration Department of Physics, Columbia University Abstract. The single largest background to future ¯ ν µ → ¯ ν e (ν µ → ν e ) oscillation searches is neutral current π 0 production. MiniBooNE, which began taking antineutrino data in January 2006, has the world's largest sample of π 0 's produced by antineutrinos in the 1 GeV energy range. These neutral pions are primarily produced through

  2. Sylvania Corporation, Hicksville, NY and Bayside NY | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Sylvania Corporation, Hicksville, NY and Bayside NY Sylvania Corporation, Hicksville, NY and Bayside NY Sylvania Corporation, Hicksville, NY and Bayside NY. Memorandumtoreader.pd...

  3. Sylvania Corporation, Hicksville, NY and Bayside, NY | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    NY Sylvania Corporation, Hicksville, NY and Bayside, NY Sylvania Corporation, Hicksville, NY and Bayside, NY, July 8, 2004. sylvaniacorporation.pdf PDF icon Sylvania Corporation, ...

  4. Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    NY - Addendum to July 8, 2004 Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum to July 8, 2004 Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum to ...

  5. Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum to July

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8, 2004 | Department of Energy NY - Addendum to July 8, 2004 Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum to July 8, 2004 Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum to July 8, 2004, additional_sylvania.pdf memorandum Date: October 6, 2004 Reply to Attn of: Department of Energy Headquarters FOIA/Privacy Act Office Sylvania Corporation, Hicksville, NY and Bayside, NY - Addendum to July 8, 2004 (11.46 KB) More Documents & Publications Index2.doc

  6. Analysis of natural gases, AL, AR, FL, GA, IL, IN, IA, KY, LA, MD, MI, MS, MO, NJ, NY, NC, OH, PA, TN, VA, and WV; 1951-1991 (for microcomputers). Data file

    SciTech Connect (OSTI)

    Not Available

    1991-01-01

    The U.S. Bureau of Mines diskette contains analysis and related source data for 2,357 natural gas samples collected from miscellaneous states, which include the following states: Alabama, Arkansas (except Arkoma Basin), Florida, Georgia, Illinois, Indiana, Iowa, Kentucky, Louisiana, Maryland, Michigan, Mississippi, Missouri, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Tennessee, Virginia, and West Virginia. All samples were obtained and analyzed as part of the Bureau's investigations of occurrences of helium in natural gases of countries with free market economies. The survey has been conducted since 1917. The analysis contained on the diskette contain the full range of component analysis data. Five files are on the diskette: READ.ME, MISC.TXT, MISC.DBF, USHEANAL.DBF, and BASINCDE.TXT.

  7. NY-Sun Loan Program

    Office of Energy Efficiency and Renewable Energy (EERE)

    NY-Sun loan program is part of broader NY-Sun Initiative program to accelerate the use of solar PV across the State. In addition to cash incentives, NY-Sun Initiative also provides State sponsored...

  8. Renewable Heat NY

    Office of Energy Efficiency and Renewable Energy (EERE)

    NOTE: On August 2015, NYSERDA increased the incentive levels for technologies offered under the Renewable Heat NY program. In general, new incentives fund up to 45% of the total project cost, which...

  9. Category:Syracuse, NY | Open Energy Information

    Open Energy Info (EERE)

    KB SVLargeOffice Syracuse NY Consolidated Edison Co-NY Inc.png SVLargeOffice Syracuse... 68 KB SVMediumOffice Syracuse NY Consolidated Edison Co-NY Inc.png SVMediumOffice...

  10. ACIM-~ NY.49

    Office of Legacy Management (LM)

    ' h:. ,,, ,_" , ACIM-~ NY.49 .,. i MEMORANDUM TO: FILE DATE FE: __~-tt_c~7' e_-_~-~------- --------- "%Kf-- ---- ---i------- Current: ~~~~~~--------__---_______ xf yee, date contacted- IVPE OF OPERATION f- ------------- Research & Development 0 Production scale testing 0 Pilot Scale 0 Bench Scale Process z Theoretical Studies Sample 84 Analysis 0 Production 0 Disposal/Storage a Facility Type 0 Manufacturing 0 University 0 Research Organization 0 Government Sponsored Fat a Other

  11. Category:Rochester, NY | Open Energy Information

    Open Energy Info (EERE)

    in this category, out of 16 total. SVFullServiceRestaurant Rochester NY Consolidated Edison Co-NY Inc.png SVFullServiceRestauran... 70 KB SVQuickServiceRestaurant Rochester NY...

  12. DOE - Office of Legacy Management -- Buffalo NY Site - NY 54

    Office of Legacy Management (LM)

    ... 19, 1992 NY.54-3 - U.S. Government (Tonawanda Sub-Office) Memorandum; Hershman to Smith; Subject: Transmittal of Monthly Progress Report for October, October 21, 1952. ...

  13. DOE - Office of Legacy Management -- Guterl Specialty Steel - NY 12

    Office of Legacy Management (LM)

    Guterl Specialty Steel - NY 12 FUSRAP Considered Sites Guterl Specialty Steel, NY Alternate Name(s): Simonds Saw and Steel Co. Guterl Steel Allegheny Ludlum Steel Corp. NY.12-1 NY.12-2 Location: Ohio Street and Route 95, Lockport, New York NY.12-12 Historical Operations: Performed rolling mill operations on natural uranium and thorium metal. NY.12-6 NY.12-7 Eligibility Determination: NY.12-11 Radiological Survey(s): Assessment Surveys NY.12-1 NY.12-4 NY.12-8 NY.12-9 NY.12-12 Site Status: Cleanup

  14. NY Solar Map and Portal

    Broader source: Energy.gov [DOE]

    The NY Solar Map and Portal helps New Yorkers determine the advantages of going solar by providing detailed and localized information about a customer's solar potential. Supported by the SunShot...

  15. Category:Greensboro, NC | Open Energy Information

    Open Energy Info (EERE)

    Greensboro NC Duke Energy Carolinas LLC.png SVQuickServiceRestaura... 68 KB SVHospital Greensboro NC Duke Energy Carolinas LLC.png SVHospital Greensboro ... 67...

  16. AER NY Kinetics LLC | Open Energy Information

    Open Energy Info (EERE)

    AER NY Kinetics LLC Jump to: navigation, search Name: AER NY Kinetics LLC Address: PO Box 585 21 Entrance Avenue Place: Ogdensburg Zip: 13669 Region: United States Sector: Marine...

  17. Western NY Energy LLC | Open Energy Information

    Open Energy Info (EERE)

    search Name: Western NY Energy LLC Place: Mount Morris, New York Zip: 14510 Product: Bioethanol producer. References: Western NY Energy LLC1 This article is a stub. You can help...

  18. Upstate NY Power Corp | Open Energy Information

    Open Energy Info (EERE)

    NY Power Corp Jump to: navigation, search Name: Upstate NY Power Corp Place: West Seneca, New York Zip: 14224-3454 Sector: Wind energy Product: Developer of clean energy projects...

  19. DOE - Office of Legacy Management -- Syracuse University - NY 29

    Office of Legacy Management (LM)

    Syracuse University - NY 29 FUSRAP Considered Sites Site: SYRACUSE UNIVERSITY (NY.29) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Syracuse , New York NY.29-1 Evaluation Year: 1994 NY.29-2 Site Operations: Activities included work with uranium oxide and the precipitation of thorium iodate from homogeneous solution. NY.29-1 NY.29-3 NY.29-4 Site Disposition: Eliminated - Potential for contamination remote NY.29-2 Radioactive Materials

  20. NC CSC Open Science Conference

    Broader source: Energy.gov [DOE]

    This three-day conference will bring the regional climate research community (North Central region universities, DOI agencies, and other research institutions) and stakeholders, including local, federal, and tribal resource managers and leaders, to foster productive engagement, interactions, and involvement with the North Central Climate Science Center (NC CSC). The conference will strive to identify emerging research issues and topics.

  1. DOE - Office of Legacy Management -- Colonie - NY 06

    Office of Legacy Management (LM)

    Colonie - NY 06 FUSRAP Considered Sites Colonie, NY Alternate Name(s): Colonie Interim Storage Site National Lead Industries NY.06-1 Location: 1130 Central Avenue, Colonie, New York NY.06-1 Historical Operations: Fabricated and processed uranium metal for the AEC, resulting in contamination from thorium and natural, enriched, and depleted uranium. NY.06-1 NY.06-4 NY.06-5 Eligibility Determination: Eligible NY.06-2 NY.06-3 Radiological Survey(s): Assessment Surveys, Verification Surveys NY.06-6

  2. DOE - Office of Legacy Management -- Gleason Works - NY 55

    Office of Legacy Management (LM)

    Gleason Works - NY 55 FUSRAP Considered Sites Site: GLEASON WORKS (NY.55 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Rochester , New York NY.55-1 Evaluation Year: 1994 NY.55-2 Site Operations: Metal fabrication operations - Rolled uranium metal. NY.55-1 Site Disposition: Eliminated - Potential for contamination considered remote NY.55-2 Radioactive Materials Handled: Yes Primary Radioactive Materials Handled: Uranium NY.55-1

  3. DOE - Office of Legacy Management -- Hooker Chemical Co - NY 05

    Office of Legacy Management (LM)

    Hooker Chemical Co - NY 05 FUSRAP Considered Sites Site: Hooker Chemical Co. (NY.05) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Occidental Chemical Corporation Hooker Electrochemical Corporation NY.05-1 NY.05-2 Location: Niagara Falls , New York NY.05-3 Evaluation Year: 1985 NY.05-1 NY.05-2 Site Operations: Design, engineering, construction, equipping and operation of a plant for the manufacture of Product 45 (xylene hexachloride); MFL (Miller's

  4. NY_8076404_nightfall.jpg | OSTI, US Dept of Energy Office of Scientific and

    Office of Scientific and Technical Information (OSTI)

    Technical Information NY_8076404_nightfall

  5. NY Green Bank | Department of Energy

    Broader source: Energy.gov (indexed) [DOE]

    sector to address and alleviate market and financial barriers preventing a thriving clean energy marketplace. NY Green Bank does not accept deposits or offer retail loans, and...

  6. DOE - Office of Legacy Management -- ACF Industries Inc - NY...

    Office of Legacy Management (LM)

    No radioactive materials used at the site. NY.13-2 Site Disposition: Eliminated - Declared clean by AEC and returned to the prime tenant NY.13-1 NY.13-3 Radioactive Materials ...

  7. NY

    Office of Legacy Management (LM)

    ... Knolls Atomic Power Laboratory (KAPL). Receipt of radioactive waete was diecontinued in ... Confi'metion of January la8l Survey Reeults Ganrna-Ray Exposure Rates The expoeure rateo ...

  8. DOE - Office of Legacy Management -- Columbia University - NY 03

    Office of Legacy Management (LM)

    Columbia University - NY 03 FUSRAP Considered Sites Site: Columbia University (NY.03 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.03-1 Evaluation Year: 1985 NY.03-2 NY.03-3 Site Operations: Early research and development -- nuclear chain reaction (fission) and gaseous diffusion during the 1940s. NY.03-4 Site Disposition: Eliminated - Radiation levels below criteria - Indication of university sponsored

  9. DOE - Office of Legacy Management -- Markite Co - NY 49

    Office of Legacy Management (LM)

    Markite Co - NY 49 FUSRAP Considered Sites Site: MARKITE CO. (NY.49 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 155 Waverly Place , New York , New York NY.49-1 Evaluation Year: 1987 NY.49-2 Site Operations: Conducted experiments with very small amounts of uranium and thorium. NY.49-2 Site Disposition: Eliminated - Handled limited amounts of radioactive materials - Potential for contamination remote NY.49-2 Radioactive Materials

  10. US MidAtl NY Site Consumption

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MidAtl NY Site Consumption million Btu 0 500 1,000 1,500 2,000 2,500 3,000 US ... 8,000 10,000 12,000 US MidAtl NY Site Consumption kilowatthours 0 250 500 750 1,000 ...

  11. New York Battery and Energy Storage Technology Consortium NY...

    Open Energy Info (EERE)

    Battery and Energy Storage Technology Consortium NY BEST Jump to: navigation, search Name: New York Battery and Energy Storage Technology Consortium (NY-BEST) Place: Albany, New...

  12. DOE - Office of Legacy Management -- National Carbon Co - NY 48

    Office of Legacy Management (LM)

    Carbon Co - NY 48 FUSRAP Considered Sites Site: NATIONAL CARBON CO (NY.48) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.48-1 Evaluation Year: 1987 NY.48-2 Site Operations: Produced graphite for the MED/AEC. NY.48-1 NY.48-2 NY.48-3 Site Disposition: Eliminated - Potential for residual radioactive contamination considered remote - No indication that radioactive material was used on the site NY.48-2 Radioactive

  13. DOE - Office of Legacy Management -- New York University - NY 50

    Office of Legacy Management (LM)

    University - NY 50 FUSRAP Considered Sites Site: NEW YORK UNIVERSITY (NY.50) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.50-1 Evaluation Year: 1987 NY.50-1 Site Operations: Activities were related to equipment development. Counters and a small quantity of uranium oxide were provided by the AEC for work under contract AT(30-1)-1256. NY.50-2 NY.50-3 NY.50-4 NY.50-1 Site Disposition: Eliminated - Potential for

  14. NC Sustainable Energy Association | Open Energy Information

    Open Energy Info (EERE)

    Jump to: navigation, search Name: NC Sustainable Energy Association Address: PO Box 6465 Place: Raleigh Zip: 27628 Number of Employees: 1-10 Year Founded: 1978 Phone...

  15. DOE - Office of Legacy Management -- American Railway Express Office - NY

    Office of Legacy Management (LM)

    0-03 Railway Express Office - NY 0-03 FUSRAP Considered Sites Site: American Railway Express Office (NY.0-03 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: American Railway Express (Downtown) , New York , New York NY.0-03-1 Evaluation Year: 1987 NY.0-03-1 Site Operations: None - Involved with a fire during transport of uranium scrap. NY.0-03-2 Site Disposition: Eliminated - Potential for contamination remote NY.0-03-1 NY.0-03-2

  16. DOE - Office of Legacy Management -- Electromet Corporation - NY 04

    Office of Legacy Management (LM)

    Electromet Corporation - NY 04 FUSRAP Considered Sites Site: Electromet Corporation (NY.04 ) Eliminated from consideration under FUSRAP - Referred to US EPA and New York State Designated Name: Not Designated Alternate Name: None Location: 4625 Royal Avenue , Niagara Falls , New York NY.04-1 Evaluation Year: 1985 NY.04-2 NY.04-3 Site Operations: Cast zirconium sponge into ingots in the 1950s. NY.04-4 Site Disposition: Eliminated - No Authority NY.04-2 Radioactive Materials Handled: Yes Primary

  17. DOE - Office of Legacy Management -- Seaway Industrial Park - NY 09

    Office of Legacy Management (LM)

    Seaway Industrial Park - NY 09 FUSRAP Considered Sites Seaway Industrial Park, NY Alternate Name(s): Seaway Industrial Landfill Seaway Landfill Charles St. Plant NY.09-2 NY.09-3 Location: River Road, Tonawanda, New York NY.09-4 Historical Operations: Received approximately 6,000 cubic yards of low-grade uranium mill tailings and processing residues from the Ashland (Tonawanda North Units 1 and 2) sites. NY.09-5 Eligibility Determination: Eligible NY.09-1 Radiological Survey(s): Assessment

  18. DOE - Office of Legacy Management -- Sylvania Corning Plant - NY 19

    Office of Legacy Management (LM)

    Plant - NY 19 FUSRAP Considered Sites Sylvania-Corning, NY Alternate Name(s): Sylvania Electric Products, Inc. Sylvania Corp. NY.19-1 NY.19-4 Location: Cantiaque Road, Hicksville, Long Island, New York NY.19-5 Historical Operations: Pilot-scale production of powdered metal uranium slugs for AEC's Hanford reactor. NY.19-4 Eligibility Determination: Eligible Radiological Survey(s): Assessment Survey NY.19-3 Site Status: Cleanup in progress by U.S. Army Corps of Engineers. USACE Website Long-term

  19. DOE - Office of Legacy Management -- Buflovak Co - NY 56

    Office of Legacy Management (LM)

    Buflovak Co - NY 56 FUSRAP Considered Sites Site: Buflovak Co. (NY.56 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 1543 Fillmore Ave. , Buffalo , New York NY.56-1 Evaluation Year: 1991 NY.56-2 Site Operations: Research and testing with uranium raffinate. NY.56-1 Site Disposition: Eliminated - Possibility for contamination considered remote due to scope of tests conducted and indication of cleanup operations after tests

  20. DOE - Office of Legacy Management -- Polytechnic Institute of Brooklyn - NY

    Office of Legacy Management (LM)

    0-19 Polytechnic Institute of Brooklyn - NY 0-19 FUSRAP Considered Sites Site: NY.0-19 (POLYTECHNIC INSTITUTE OF BROOKLYN) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.0-19-1 Evaluation Year: 1987 NY.0-19-1 Site Operations: Research and development involving only small quantities of radiological material in a controlled environment. NY.0-19-1 Site Disposition: Eliminated - Potential for contamination remote

  1. DOE - Office of Legacy Management -- Radiation Applications Inc - NY 57

    Office of Legacy Management (LM)

    Radiation Applications Inc - NY 57 FUSRAP Considered Sites Site: RADIATION APPLICATIONS, INC. ( NY.57 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: RAI NY.57-1 Location: 370 Lexington Avenue , New York , New York NY.57-3 Evaluation Year: 1991 NY.57-4 Site Operations: Developed foam separation techniques and proposed investigations to remove cesium and strontium from fission product waste solutions. No indication that a substantial quantity of

  2. DOE - Office of Legacy Management -- Sacandaga - NY 51

    Office of Legacy Management (LM)

    Sacandaga - NY 51 FUSRAP Considered Sites Site: SACANDAGA (NY.51) Eliminated - Condition certified Designated Name: Not Designated Alternate Name: None Location: 823 Sacandaga Road , Glenville , New York NY.51-1 Evaluation Year: 1992 NY.51-2 Site Operations: Plant operated by General Electric during period spanning 1947 to 1951. Facilities housed studies involving radar, physics studies and sodium technology development. NY.51-1 Site Disposition: Eliminated - Radiation levels below criteria

  3. DOE - Office of Legacy Management -- Youngsville Warehouse - NC 02

    Office of Legacy Management (LM)

    Youngsville Warehouse - NC 02 FUSRAP Considered Sites Site: Youngsville Warehouse (NC.02) Eliminated from consideration under FUSRAP - No AEC involvement indicated Designated Name: Not Designated Alternate Name: General Atomic NC.02-1 Location: Youngsville , North Carolina NC.02-2 Evaluation Year: Not Applicable - No record that cleanup of the site under FUSRAP was formally considered. Site Operations: Thorium storage facility. NC.02-3 NC.02-4 Site Disposition: Eliminated - Commercial operation

  4. DOE - Office of Legacy Management -- North Carolina State University - NC

    Office of Legacy Management (LM)

    01 Carolina State University - NC 01 FUSRAP Considered Sites Site: NORTH CAROLINA STATE UNIVERSITY (NC.01) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: North Carolina State College NC.01-1 Location: Raleigh , North Carolina NC.01-1 Evaluation Year: 1993 NC.01-2 Site Operations: Performed investigative research concerning the measurement of the thermal properties of certain granular materials in 1953 and 1954. NC.01-1 NC.01-4 NC.01-5 Site

  5. The 1/Nc Expansion in Baryons

    SciTech Connect (OSTI)

    Jose L. Goity, Chandana Jayalath, Norberto N. Scoccola

    2010-08-01

    The 1/N_c expansion in baryons is discussed with two applications, namely baryon masses and partial decay widths. These applications provide the basic insights on the utility of the expansion.

  6. NC GreenPower Production Incentive

    Broader source: Energy.gov [DOE]

    NC GreenPower, a statewide green power program designed to encourage the use of renewable energy in North Carolina, offers production payments for grid-tied electricity generated by solar, wind, ...

  7. DOE - Office of Legacy Management -- Staten Island Warehouse - NY 22

    Office of Legacy Management (LM)

    Staten Island Warehouse - NY 22 FUSRAP Considered Sites Staten Island Warehouse, NY Alternate Name(s): Archer-Daniels Midland Company NY.22-3 Location: 2393 Richmond Terrace, Port Richmond, New York NY.22-2 Historical Operations: Stored pitchblende (high-grade uranium ore), which was purchased by the MED for the first atomic bomb. NY.22-3 Eligibility Determination: Eligible Radiological Survey(s): Assessment Survey NY.22-5 Site Status: Referred by DOE, evaluation in progess by U.S. Army Corps of

  8. DOE - Office of Legacy Management -- Love Canal - NY 24

    Office of Legacy Management (LM)

    NY.24-1 - DOE MemorandumChecklist; Jones to File; Subject: Elimination - Love Canal; April 30, 1987 NY.24-3 - Report; Smith to Fink; Preliminary Report of New York State Assembly ...

  9. DOE - Office of Legacy Management -- Ledoux and Co - NY 37

    Office of Legacy Management (LM)

    Ledoux and Co - NY 37 FUSRAP Considered Sites Site: LEDOUX AND CO. (NY.37 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 155 Avenue of the Americas , New York , New York NY.37-1 Evaluation Year: 1987 NY.37-1 Site Operations: Prime contractor to AEC, African Metals. LeDoux handled radioactive materials under this contract at other locations; records indicate that radioactive materials were not sent to the New York office.

  10. DOE - Office of Legacy Management -- Rensselaer Polytechnic Institute - NY

    Office of Legacy Management (LM)

    18 Rensselaer Polytechnic Institute - NY 18 Site ID (CSD Index Number): NY.18 Site Name: RENSSELAER POLYTECHNIC INSTITUTE Site Summary: Site Link: External Site Link: Alternate Name(s): None Alternate Name Documents: Location: Troy , New York Location Documents: NY.18-1 Historical Operations (describe contaminants): Research activities involving small quantities of radioactive materials in a controlled environment - under AEC license. Historical Operations Documents: NY.18-1 Eligibility

  11. DOE - Office of Legacy Management -- Seneca Army Depot - NY 11

    Office of Legacy Management (LM)

    Seneca Army Depot - NY 11 FUSRAP Considered Sites Site: SENECA ARMY DEPOT (NY.11 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Romulus , New York Evaluation Year: 1985 NY.11-2 NY.11-3 Site Operations: Eleven bunkers were used to store approximately 2,000 drums of pitchblende ore in the early 1940's. The bunkers were returned to munitions storage service after removal of the ore drums. NY.11-4 Site Disposition: Eliminated - Referred to

  12. NlZWYORK4,N.Y.

    Office of Legacy Management (LM)

    AFRICAN .METALS~ C~RPO~~XON 41 BROAD STREET . i. ,,J iI: : LE OCT 2 2 1945 NlZWYORK4,N.Y. :October 5, 1945. Af-2-a L.: I.__: '../ . ._ The Area Engineer, U.S. Engineer Office, P.O. BOX 42, Station F., New York 16, N.Y. Gentlemen: Contract W-7405 eng-4. Reference is made to your letter EIDM A-33 MS of August 27th, 1945. Contract W-7405 eng-4 called for the delivery of 100 T of M-31, the M308 content of which was sold to you, whereas we reserved all rights to the R-l contained therein. We hereby

  13. DOE - Office of Legacy Management -- American Machine and Foundry Co - NY

    Office of Legacy Management (LM)

    26 NY 26 FUSRAP Considered Sites Site: American Machine and Foundry Co ( NY.26 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Lutheran Medical Center NY.26-1 Location: Second Avenue and 56th Street , Brooklyn , New York NY.26-2 Evaluation Year: 1992 NY.26-1 Site Operations: 1951 - 1954 conducted metal fabrication operation on uranium and thorium metals. NY.26-3 NY.26-4 Site Disposition: Eliminated - Potential for contamination considered remote

  14. NC-1-B Wholesale Power Rate Schedule | Department of Energy

    Energy Savers [EERE]

    NC-1-B Wholesale Power Rate Schedule NC-1-B Wholesale Power Rate Schedule Area: Virginia PowerCP&L System: Kerr-Philpott This rate schedule shall be available to public bodies and...

  15. N.C. Solar Center | Open Energy Information

    Open Energy Info (EERE)

    C. Solar Center Jump to: navigation, search Name: N.C. Solar Center Address: NCSU, Box 7401 Place: Raleigh, NC Zip: 27695 Number of Employees: 11-50 Year Founded: 1988 Website:...

  16. Measurement of the direct <mi>CP> -violating parameter <mi>Ami><mi>CP> in the decay <mi>D>+<mi>Kmi>-<mimi>+<mi>π>+

    SciTech Connect (OSTI)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2014-12-01

    We measure the direct mi>Cmi>mi>P>-violating parameter mi>Ami>mi>Cmi>mi>Pmi> for the decay of the charged charm meson, mi>Dmi>+mi>Kmi>-mi>πmi>+mi>πmi>+ (and charge conjugate), using the full 10.4 mi>fbmi>-1 sample of mi>p>mi>p>¯ collisions at mi>smi>=1.96 mi>TeVmi> collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from mi>B>mi>D

  17. Business Council of Westchester County (NY) | Open Energy Information

    Open Energy Info (EERE)

    101 Place: White Plains, New York Zip: 10604 Region: Northeast - NY NJ CT PA Area Sector: Services Product: Green Power Marketer Website: www.westchesterny.org Coordinates:...

  18. NY-Sun PV Incentive Program (Residential and Small Business)...

    Broader source: Energy.gov (indexed) [DOE]

    NY-Sun CommercialIndustrial Incentive program that offers incentives for grid connected PV systems larger than 200 KW. The New York State Energy Research and Development...

  19. DOE Zero Energy Ready Home Case Study: AquaZephyr, Ithaca, NY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AquaZephyr, Ithaca, NY DOE Zero Energy Ready Home Case Study: AquaZephyr, Ithaca, NY Case study of a DOE Zero Energy Ready home in Ithaca, NY, that achieves a HERS 56 without PV or ...

  20. DOE - Office of Legacy Management -- Crucible Steel Co of America - NY 34

    Office of Legacy Management (LM)

    Crucible Steel Co of America - NY 34 FUSRAP Considered Sites Site: Crucible Steel Co. of America (NY.34 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Syracuse , New York NY.34-1 Evaluation Year: 1987 NY.34-1 Site Operations: Research and Development; Small amount of Thorium forged and rolled; Large amounts of non-radioactive metals provided to the AEC. NY.34-1 Site Disposition: Eliminated - Potential for contamination remote NY.34-1

  1. DOE - Office of Legacy Management -- Enterprise Metal Products - NY 0-10

    Office of Legacy Management (LM)

    Enterprise Metal Products - NY 0-10 FUSRAP Considered Sites Site: Enterprise Metal Products (NY.0-10 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Brooklyn , New York NY.0-10-1 Evaluation Year: 1987 NY.0-10-1 Site Operations: Machined magnesium metal NY.0-10-1 Site Disposition: Eliminated - Potential for contamination remote NY.0-10-1 Radioactive Materials Handled: None Indicated Primary Radioactive Materials Handled: None Indicated

  2. DOE - Office of Legacy Management -- Fordham University - NY 0-12

    Office of Legacy Management (LM)

    Fordham University - NY 0-12 FUSRAP Considered Sites Site: Fordham University (NY.0-12 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.0-12-1 Evaluation Year: 1987 NY.0-12-1 Site Operations: Research and development involving small quantities of radioactive material in a controlled environment NY.0-12-1 Site Disposition: Eliminated - Potential for contamination remote NY.0-12-1 Radioactive Materials Handled: Yes

  3. DOE - Office of Legacy Management -- Linde Air Products Div - Buffalo - NY

    Office of Legacy Management (LM)

    65 Div - Buffalo - NY 65 FUSRAP Considered Sites Site: LINDE AIR PRODUCTS DIV. BUFFALO (NY.65 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: Linde Chandler Street Plant NY.65-1 Location: Buffalo , New York NY.65-1 Evaluation Year: 1987 NY.65-1 Site Operations: Developed and produced non-radioactive material for the Oak Ridge Gaseous Diffusion Plant under contract with the AEC. NY.65-2 Site Disposition: Eliminated - No indication that

  4. DOE - Office of Legacy Management -- Simmons Machine and Tool Inc - NY 35

    Office of Legacy Management (LM)

    Simmons Machine and Tool Inc - NY 35 FUSRAP Considered Sites Site: SIMMONS MACHINE AND TOOL, INC (NY.35) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 1000 North Broadway , Albany , New York NY.35-1 Evaluation Year: 1987 NY.35-2 Site Operations: Tested equipment and machined uranium to test the equipment (one time event). NY.35-1 NY.35-2 Site Disposition: Eliminated - Potential for contamination considered remote due to limited scope

  5. DOE - Office of Legacy Management -- Eastman Kodak Laboratory - NY 0-09

    Office of Legacy Management (LM)

    Eastman Kodak Laboratory - NY 0-09 FUSRAP Considered Sites Site: Eastman Kodak Laboratory (NY.0-09 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Eastman Kodak Rochester Lab NY.0-09-1 Location: Rochester , New York NY.0-09-1 Evaluation Year: 1987 NY.0-09-1 NY.0-09-2 Site Operations: Research and development with natural uranium solutions in 1943. NY.0-09-1 Site Disposition: Eliminated - Potential for contamination remote NY.0-09-2 Radioactive

  6. DOE - Office of Legacy Management -- Linde Air Products Division - NY 08

    Office of Legacy Management (LM)

    Division - NY 08 FUSRAP Considered Sites Linde Air Products Division - Towanda, NY Alternate Name(s): Praxair Linde Aire Products Div. of Union Carbide Corp. Linde Ceramics Plant Uranium Refinery, Linde Site NY.08-4 Location: East Park Drive and Woodward, Tonawanda, New York NY.08-5 Historical Operations: Processed uranium compounds for MED and AEC. Includes Towanda Landfill as a VP. NY.08-1 NY.08-2 Eligibility Determination: Eligible NY.08-9 Radiological Survey(s): Assessment Surveys NY.08-3

  7. DOE - Office of Legacy Management -- Radium Chemical Co Inc - NY 60

    Office of Legacy Management (LM)

    Radium Chemical Co Inc - NY 60 FUSRAP Considered Sites Site: RADIUM CHEMICAL CO., INC (NY.60 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.60-1 Evaluation Year: 1987 NY.60-1 Site Operations: Commercial Producer of Radium. NY.60-1 Site Disposition: Eliminated - Commercial site - EPA cleanup project NY.60-1 Radioactive Materials Handled: Yes NY.60-1 Primary Radioactive Materials Handled: Radium NY.60-1

  8. DOE - Office of Legacy Management -- Ferro Metal and Chemical Co - NY 42

    Office of Legacy Management (LM)

    Ferro Metal and Chemical Co - NY 42 FUSRAP Considered Sites Site: Ferro Metal & Chemical Co. (NY.42 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 50 Broad Street , New York , New York NY.42-1 NY.42-2 Evaluation Year: 1987 NY.42-2 Site Operations: Procured uranium from foreign sources. No record of radioactive material at this site NY.42-1 NY.42-2 Site Disposition: Eliminated - No indication that radioactive materials were used at

  9. DOE - Office of Legacy Management -- Floyd Bennett Field - NY 0-11

    Office of Legacy Management (LM)

    Floyd Bennett Field - NY 0-11 FUSRAP Considered Sites Site: Floyd Bennett Field (NY.0-11 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Naval Air Station NY.0-11-1 Location: Buildings 67 and 69 , Brooklyn , New York NY.0-11-1 Evaluation Year: 1987 NY.0-11-1 Site Operations: The Air station was considered by the AEC but was not used. NY.0-11-1 Site Disposition: Eliminated - No involvement with MED/AEC operations NY.0-11-1 Radioactive Materials

  10. DOE - Office of Legacy Management -- Union Mines Development Corp - NY 0-22

    Office of Legacy Management (LM)

    Mines Development Corp - NY 0-22 FUSRAP Considered Sites Site: UNION MINES DEVELOPMENT CORP. (NY.0-22) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Union Carbide NY.0-22-1 Location: New York , New York NY.0-22-1 Evaluation Year: 1987 NY.0-22-1 Site Operations: The company owned uranium mines or reserves located in the western U.S. NY.0-22-1 Site Disposition: Eliminated - No reason to believe radioactive material was used at this site NY.0-22-1

  11. DOE - Office of Legacy Management -- Colonie - NY 06

    Office of Legacy Management (LM)

    of Engineers but will eventually transfer to the U.S. Department of Energy Office of Legacy Management. NY.06-1 - DOE Report (DOEOR20722-53); Colonie Interim Storage Site...

  12. NY-Sun Commerical/Industrial Incentive Program

    Broader source: Energy.gov [DOE]

    New York State Energy Research and Development Authority (NYSERDA) through NY-Sun Commercial/Industrial Incentive Program (PON 3082) provides incentives for installation of non-residential new grid...

  13. RenewableNY - An Industrial Energy Conservation Initiative

    SciTech Connect (OSTI)

    Lubarr, Tzipora

    2009-09-30

    The New York Industrial Retention Network (NYIRN) manages the RenewableNY program to assist industrial companies in New York City to implement energy efficiency projects. RenewableNY provides companies with project management assistance and grants to identify opportunities for energy savings and implement energy efficiency projects. The program helps companies identify energy efficient projects, complete an energy audit, and connect with energy contractors who install renewable energy and energy efficient equipment. It also provides grants to help cover the costs of installation for new systems and equipment. RenewableNY demonstrates that a small grant program that also provides project management assistance can incentivize companies to implement energy efficiency projects that might otherwise be avoided. Estimated savings through RenewableNY include 324,500 kWh saved through efficiency installations, 158 kW of solar energy systems installed, and 945 thm of gas avoided.

  14. DOE - Office of Legacy Management -- Ithaca Gun Co Inc - NY 53

    Office of Legacy Management (LM)

    Ithaca Gun Co Inc - NY 53 FUSRAP Considered Sites Site: ITHACA GUN CO., INC. (NY.53 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 123 Lake Street , Ithaca , New York NY.53-1 Evaluation Year: 1994 NY.53-2 Site Operations: Conducted tests involving the forging of hollow uranium billets into tubes for the AEC. Also investigated alternative methods of producing fuel cores. NY.53-1 Site Disposition: Eliminated - No radioactive

  15. DOE - Office of Legacy Management -- Utica Street Warehouse - NY 0-23

    Office of Legacy Management (LM)

    Street Warehouse - NY 0-23 FUSRAP Considered Sites Site: UTICA STREET WAREHOUSE (NY.0-23) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 240 West Utica Street , Buffalo , New York NY.0-23-2 Evaluation Year: 1987 NY.0-23-1 Site Operations: Stored and rebarrelled uranium process residues from operations at Linde. NY.0-23-3 Site Disposition: Eliminated - Original building demolished. Current land use - Parking facility. Potential for

  16. New York State Energy Research and Development Authority, Albany, NY |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy York State Energy Research and Development Authority, Albany, NY New York State Energy Research and Development Authority, Albany, NY This presentation on the NYSERDA Hydrogen Program was given on July 12, 2006. education_presentation_nyserda.pdf (2.64 MB) More Documents & Publications NYSERDA's RPS Customer Sited Tier Fuel Cell Program State of the States: Fuel Cells in America 2014 State of the States: Fuel Cells in America 2010

  17. Los Alamos technology to be featured on CSI: NY

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    LANL technology featured on CSI: NY Los Alamos technology to be featured on CSI: NY The multipurpose "sampler gun" rapidly collects and tracks radiological, chemical, and biological samples in solid, liquid, or gaseous forms. March 27, 2008 Los Alamos National Laboratory sits on top of a once-remote mesa in northern New Mexico with the Jemez mountains as a backdrop to research and innovation covering multi-disciplines from bioscience, sustainable energy sources, to plasma physics and

  18. DOE - Office of Legacy Management -- Pfohl Brothers Landfill - NY 66

    Office of Legacy Management (LM)

    Pfohl Brothers Landfill - NY 66 FUSRAP Considered Sites Site: Pfohl Brothers Landfill (NY.66 ) Designated Name: Alternate Name: Location: Evaluation Year: Site Operations: Site Disposition: Radioactive Materials Handled: Primary Radioactive Materials Handled: Radiological Survey(s): Site Status: Also see Five-Year Review Report Pfohl Brothers Landfill Superfund Site Erie County Town of Cheektowaga, New York EPA REGION 2 Congressional District(s): 30 Erie Cheektowaga NPL LISTING HISTORY Documents

  19. shaleusa5.pdf

    Gasoline and Diesel Fuel Update (EIA)

    ... NY PA NJ OH DC DE WV MD KY VA NC TN SC MI CANADA 2 0 0 1 0 0 Marcellus Shale Gas Play, Appalachian Basin Source: US Energy Information Administration based on data from WVGES , PA ...

  20. Origin State>> CA CA ID ID ID IL KY MD MO NM NM NY NY OH SC

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MO NM NM NY NY OH SC TN TN TN, WA, CA TN TN TN TN Total Shipments by Route Lawrence Livermore National Laboratory General Atomics Batelle Energy Alliance Idaho National Laboratory Advanced Mixed Waste Treatment Project Argonne National Laboratory Paducah Gaseous Diffusion Plant Aberdeen Proving Grounds National Security Technologies Sandia National Laboratory Los Alamos National Laboratory Brookhaven National Laboratory CH2M Hill B&W West Valley, LLC Portsmouth Gaseous Diffusion Plant

  1. Origin State>> CA CA ID ID ID IL KY MD NM NM NV NY NY OH TN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NM NM NV NY NY OH TN TN TN, WA, CA TN TN TN TN TX Total Shipments by Route Lawrence Livermore National Laboratory General Atomics Advanced Mixed Waste Treatment Project Batelle Energy Alliance Idaho National Laboratory Argonne National Laboratory Paducah Gaseous Diffusion Plant Aberdeen Proving Ground Los Alamos National Laboratory Sandia National Laboratory National Security Technologies Brookhaven National Laboratory West Valley Environmental Services Portsmouth Gaseous Diffusion Plant

  2. Origin State>> CA ID ID ID IL KY MD NM NM NY NY OH SC TN TN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MD NM NM NY NY OH SC TN TN TN, WA, CA TN TN TN TN TX Total Shipments by Route Lawrence Livermore National Laboratory Advanced Mixed Waste Treatment Project Batelle Energy Alliance Idaho National Laboratory Argonne National Laboratory Paducah Gaseous Diffusion Plant Aberdeen Proving Ground Los Alamos National Laboratory Sandia National Laboratory Brookhaven National Laboratory West Valley Environmental Services Portsmouth Gaseous Diffusion Plant Savannah River Site Duratek/Energy Solutions Babcox

  3. Origin State>> CA ID ID ID IL KY NV NY NY OH TN TN TN, WA, CA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NV NY NY OH TN TN TN, WA, CA TN TN TN TN TX Total Shipments by Route Lawrence Livermore National Laboratory Advanced Mixed Waste Treatment Project Batelle Energy Alliance Idaho National Laboratory Argonne National Laboratory Paducah Gaseous Diffusion Plant National Security Technologies Brookhaven National Laboratory West Valley Environmental Services Portsmouth Gaseous Diffusion Plant Duratek/Energy Solutions Babcox & Wilcox Technical Services Y-12 Plant Materials & Energy Corporation

  4. Origin State>> CA ID ID ID IL MD NM NM NV NY NY OH SC TN TN

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NV NY NY OH SC TN TN TN, WA, CA TN TN TN Total Shipments by Route Lawrence Livermore National Laboratory Batelle Energy Alliance Idaho National Laboratory Advanced Mixed Waste Treatment Project Argonne National Laboratory Aberdeen Proving Ground Sandia National Laboratory Los Alamos National Laboratory National Security Technologies Brookhaven National Laboratory West Valley Environmental Services Portsmouth Gaseous Diffusion Plant Savannah River Site Duratek/Energy Solutions Babcox & Wilcox

  5. NC GreenPower Renewable Energy Credit Production

    Broader source: Energy.gov [DOE]

    NC GreenPower is requesting proposals for renewable energy credits associated with renewable energy, such as solar, PV, wind, small hydro of 10 MW or less, generated in North Carolina and supplied to the North Carolina electric grid.

  6. DOE - Office of Legacy Management -- African Metals - NY 0-01

    Office of Legacy Management (LM)

    African Metals - NY 0-01 FUSRAP Considered Sites Site: African Metals (NY 0-01) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Afrimet NY.0-01-1 Location: 41 Broad Street , New York , New York NY.0-01-2 Evaluation Year: 1987 NY.0-01-1 Site Operations: U.S. Agent for Union Miniere du Haut Katanga; site of the main corporate offices; no radioactive materials handled at this location. NY.0-01-1 Site Disposition: Eliminated - No potential for

  7. DOE - Office of Legacy Management -- Colorado Fuel and Iron - NY 0-08

    Office of Legacy Management (LM)

    Fuel and Iron - NY 0-08 FUSRAP Considered Sites Site: Colorado Fuel and Iron (NY.0-08 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Watervliet , New York NY.0-08-1 Evaluation Year: 1987 NY.0-08-1 Site Operations: Site was a contractor to DuPont. Exact nature of operations is not clear. No records to indicate that radioactive materials were handled at the site. NY.0-08-1 Site Disposition: Eliminated NY.0-08-1 Radioactive Materials

  8. DOE - Office of Legacy Management -- Frederick Flader Inc - NY 0-13

    Office of Legacy Management (LM)

    Frederick Flader Inc - NY 0-13 FUSRAP Considered Sites Site: Frederick Flader, Inc. (NY.0-13 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Frederick Flader Division of Eaton Manufacturing Co. NY.0-13-1 Location: 583 Division Street , N. Tonawanda , New York NY.0-13-1 Evaluation Year: 1987 NY.0-13-1 Site Operations: Provided consulting services and supported development of auxiliary equipment related to nuclear power NY.0-13-1 Site Disposition:

  9. DOE - Office of Legacy Management -- Long Island College of Medicine - NY

    Office of Legacy Management (LM)

    0-14 Long Island College of Medicine - NY 0-14 FUSRAP Considered Sites Site: Long Island College of Medicine (NY.0-14 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.0-14-1 Evaluation Year: 1987 NY.0-14-1 Site Operations: Performed research utilizing small quantities of radioactive materials in a controlled environment. NY.0-14-1 Site Disposition: Eliminated - Potential for contamination remote NY.0-14-1

  10. DOE - Office of Legacy Management -- Lucius Pitkin - NY 0-15

    Office of Legacy Management (LM)

    Lucius Pitkin - NY 0-15 FUSRAP Considered Sites Site: Lucius Pitkin (NY.0-15 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 47 Fulton Street , New York , New York NY.0-15-1 Evaluation Year: 1987 NY.0-15-1 Site Operations: No MED or AED work done at this site. Contractor supervised activities at Middlesex Sampling Plant in Middlesex, NJ such as assaying, sampling and weighing of ore. NY.0-15-1 NY.0-15-2 Site Disposition: Eliminated - No

  11. DOE - Office of Legacy Management -- Memorial Hospital - NY 0-16

    Office of Legacy Management (LM)

    Memorial Hospital - NY 0-16 FUSRAP Considered Sites Site: MEMORIAL HOSPITAL (NY.0-16 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: New York , New York NY.0-16-1 Evaluation Year: 1987 NY.0-16-1 Site Operations: Conducted studies for MED including a "Physiological Study of Sunbath Treatments." Involvement with radioactive materials unknown. NY.0-16-1 Site Disposition: Eliminated - Potential for contamination remote NY.0-16-1

  12. DOE - Office of Legacy Management -- Pyroferric Co - NY 0-20

    Office of Legacy Management (LM)

    Pyroferric Co - NY 0-20 FUSRAP Considered Sites Site: PYROFERRIC CO. (NY.0-20) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: 621 E. 216th Street , New York , New York NY.0-20-1 Evaluation Year: 1987 NY.0-20-1 Site Operations: One small scale experiment was conducted with non-radioactive material. NY.0-20-1 Site Disposition: Eliminated NY.0-20-1 Radioactive Materials Handled: No Primary Radioactive Materials Handled: None Radiological

  13. Champlain, NY Natural Gas Liquefied Natural Gas Imports (Million Cubic

    U.S. Energy Information Administration (EIA) Indexed Site

    Feet) (Million Cubic Feet) Champlain, NY Natural Gas Liquefied Natural Gas Imports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2014 63 2015 1 2 1 2 20 2016 56 76 20 20 3 4 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Imports by Point of Entry Champlain, NY LNG Imports from All Countr

  14. Submit Your Ideas for the NY Energy Data Jam | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Submit Your Ideas for the NY Energy Data Jam Submit Your Ideas for the NY Energy Data Jam June 19, 2013 - 11:03am Q&A What idea would you present at the Data Jam? Join the...

  15. DOE - Office of Legacy Management -- Knolls Atomic Power Laboratory - NY 16

    Office of Legacy Management (LM)

    Knolls Atomic Power Laboratory - NY 16 Site ID (CSD Index Number): NY.16 Site Name: Knolls Atomic Power Laboratory Site Summary: Site Link: External Site Link: http://www.knollslab.com/ Alternate Name(s): Peek Street Facility Knolls Atomic Power Lab of General Electric, Schnectady Alternate Name Documents: Location: Niskayuna, NY and West Milton, NY Location Documents: Historical Operations (describe contaminants): The site was considered because it provided support to the Atomic Energy

  16. DOE - Office of Legacy Management -- Utica Drop Forge and Tool Corp - NY 39

    Office of Legacy Management (LM)

    Drop Forge and Tool Corp - NY 39 FUSRAP Considered Sites Site: UTICA DROP FORGE & TOOL CORP. (NY.39) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Utica , New York NY.39-1 Evaluation Year: 1994 NY.39-2 Site Operations: Interest expressed by Utica Drop Forge & Tool Corporation to Conduct Uranium Forging and Casting for the AEC. No indication that the contractor actually did a substantial amount of work of this nature at

  17. Interoperability of Demand Response Resources Demonstration in NY

    SciTech Connect (OSTI)

    Wellington, Andre

    2014-03-31

    The Interoperability of Demand Response Resources Demonstration in NY (Interoperability Project) was awarded to Con Edison in 2009. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited Demand Response resources to integrate more effectively with electric delivery companies and regional transmission organizations.

  18. DOE - Office of Legacy Management -- Wolff-Alport and Co - NY 30

    Office of Legacy Management (LM)

    Wolff-Alport and Co - NY 30 FUSRAP Considered Sites Site: Wolff-Alport and Co (NY.30) Eliminated from consideration under FUSRAP - Referred to US EPA Region II and New York City Department of Health Designated Name: Not Designated Alternate Name: None Location: 1127 Irving Avenue , Brooklyn , New York NY.30-1 Evaluation Year: 1987 NY.30-1 Site Operations: Commercial operation -- sold thorium residues to the AEC, which in turn shipped the residues to Maywood for storage. NY.30-2 Site Disposition:

  19. Buffalo, NY Liquefied Natural Gas Exports (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    (Million Cubic Feet) Buffalo, NY Liquefied Natural Gas Exports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2015 1 1 1 1 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S.

  20. Data Analysis from Ground Source Heat Pump Demonstration Projects

    Office of Energy Efficiency and Renewable Energy (EERE)

    Lead Performer: Oak Ridge National Laboratory - Oak Ridge, TN Partners: -- CDH Energy - Cazenovia, NY -- University of Tennessee - Knoxville, TN -- Cedarville Schools - Cedarville, AR (ARRA grantee) -- Flathead Electric Cooperative - Kalispell, MT (ARRA grantee) -- University at Albany - Albany, NY (ARRA grantee) -- City of Raleigh, NC (ARRA grantee) -- Montana Tech (ARRA grantee) -- Oakland University - Rochester, MI (ARRA grantee)

  1. KP-NC-1-C Wholesale Power Rate Schedule | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    NC-1-C Wholesale Power Rate Schedule KP-NC-1-C Wholesale Power Rate Schedule Area: Virginia PowerDuke Energy Progress System: Kerr-Philpott This rate schedule shall be available ...

  2. DOE Zero Energy Ready Home Case Study: Greenhill Contracting, New Paltz, NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    | Department of Energy New Paltz, NY DOE Zero Energy Ready Home Case Study: Greenhill Contracting, New Paltz, NY DOE Zero Energy Ready Home Case Study: Greenhill Contracting, New Paltz, NY Case study of a DOE Zero Energy Ready home in New Paltz, NY, that achieved a HERS score of 37 without PV or 7 with 7.5-kW PV. The two-story 2,288-ft2 home is one of 9 certified homes. All of the homes have R-22 ICF walls, R-20 closed-cell spray foam under the slab, a ground-source heat pump with

  3. I?raak Nuke, NXOO Leaa Km&l, NY00

    Office of Legacy Management (LM)

    W. ti. &&n,Nor I?raak Nuke, NXOO Leaa Km&l, NY00 fjlrt~olfloatlon Camad .- ,' I i 2 Jr,' ; !

  4. Champlain, NY Natural Gas Liquefied Natural Gas Imports from Canada

    U.S. Energy Information Administration (EIA) Indexed Site

    (Million Cubic Feet) Liquefied Natural Gas Imports from Canada (Million Cubic Feet) Champlain, NY Natural Gas Liquefied Natural Gas Imports from Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2014 63 2015 1 2 1 2 20 2016 56 76 20 20 3 4 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Ga

  5. FIRST Robotics at NY Tech Valley | GE Global Research

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Pit Boss Reflects on Bot Challenge Click to email this to a friend (Opens in new window) Share on Facebook (Opens in new window) Click to share (Opens in new window) Click to share on LinkedIn (Opens in new window) Click to share on Tumblr (Opens in new window) Pit Boss Reflects on Bot Challenge Lynn DeRose 2015.03.27 Hi everyone, from BBQ to robots all in the same week! This past weekend I had the privilege to volunteer at the FIRST(tm) Robotics Competition (FRC) NY Tech Valley Regional at RPI.

  6. Microsoft PowerPoint - Xie_twpice_NY06.ppt

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Variational Analysis of Tropical Warm Pool International Cloud Experiment (TWP_ICE) Data (01/22/2006 - 2/13/2006) The TWP_ICE Workshop , NASA/GISS, New York, NY, 13-15 November, 2006 A joint effort between LLNL (Xie, McCoy, Klein), BMRC (Hume, Jakob), SUNYSB (Zhang), and other TWPICE participants 143km 130km 172km 99km 148km A Quick Update Done! 3-hourly radiosondes (6 stations) Background field from the ECMWF analysis Radar precipitation Surface turbulence fluxes from Monash University (Darwin

  7. Real-time sub-<mi>>ngstrom...

    Office of Scientific and Technical Information (OSTI)

    Real-time sub-<mi>>ngstrom imaging of reversible and irreversible conformations in rhodium catalysts and graphene Kisielowski, Christian; Wang,...

  8. Mi GmbH | Open Energy Information

    Open Energy Info (EERE)

    Mi GmbH Jump to: navigation, search Name: Mi GmbH Place: Switzerland Zip: CH-6340 Sector: Solar Product: Baar-based manufacturer and distributor of fruit juices. The firm is also...

  9. The Nc dependencies of baryon masses: Analysis with Lattice QCD and Effective Theory

    SciTech Connect (OSTI)

    Calle Cordon, Alvaro C.; DeGrand, Thomas A.; Goity, Jose L.

    2014-07-01

    Baryon masses at varying values of Nc and light quark masses are studied with Lattice QCD and the results are analyzed in a low energy effective theory based on a combined framework of the 1/Nc and Heavy Baryon Chiral Perturbation Theory expansions. Lattice QCD results for Nc=3, 5 and 7 obtained in quenched calculations, as well as results for unquenched calculations for Nc=3, are used for the analysis. The results are consistent with a previous analysis of Nc=3 LQCD results, and in addition permit the determination of sub-leading in 1/Nc effects in the spin-flavor singlet component of the baryon masses as well as in the hyperfine splittings.

  10. miRNAs in brain development

    SciTech Connect (OSTI)

    Petri, Rebecca; Malmevik, Josephine; Fasching, Liana; Åkerblom, Malin; Jakobsson, Johan

    2014-02-01

    MicroRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. In the brain, a large number of miRNAs are expressed and there is a growing body of evidence demonstrating that miRNAs are essential for brain development and neuronal function. Conditional knockout studies of the core components in the miRNA biogenesis pathway, such as Dicer and DGCR8, have demonstrated a crucial role for miRNAs during the development of the central nervous system. Furthermore, mice deleted for specific miRNAs and miRNA-clusters demonstrate diverse functional roles for different miRNAs during the development of different brain structures. miRNAs have been proposed to regulate cellular functions such as differentiation, proliferation and fate-determination of neural progenitors. In this review we summarise the findings from recent studies that highlight the importance of miRNAs in brain development with a focus on the mouse model. We also discuss the technical limitations of current miRNA studies that still limit our understanding of this family of non-coding RNAs and propose the use of novel and refined technologies that are needed in order to fully determine the impact of specific miRNAs in brain development. - Highlights: • miRNAs are essential for brain development and neuronal function. • KO of Dicer is embryonically lethal. • Conditional Dicer KO results in defective proliferation or increased apoptosis. • KO of individual miRNAs or miRNA families is necessary to determine function.

  11. Highly Excited Baryons in Large-Nc QCD

    SciTech Connect (OSTI)

    Matagne, N.; Stancu, Fl.

    2005-06-14

    We use the 1/Nc expansion of QCD to analyse the spectrum of positive parity resonances with strangeness S = 0, -1, -2 and -3 in the 2-3 GeV mass region, supposed to belong to the [56,4+] multiplet. The mass operator is similar to that of [56,2+], previously studied in the literature. The analysis of the latter is revisited. In the [56,4+] multiplet we find that the spin-spin term brings the dominant contribution and that the spin-orbit term is entirely negligible in the hyperfine interaction, in agreement with the constituent quark model practice, where this interaction is usually neglected. More data are strongly desirable, especially in the strange sector in order to fully exploit the power of this approach. We discuss possibilities of extending the calculations to other excited baryons belonging to the N = 2 or the N = 4 band.

  12. DOE - Office of Legacy Management -- Michigan Velsicol Chemical Corp - MI

    Office of Legacy Management (LM)

    03 Michigan Velsicol Chemical Corp - MI 03 FUSRAP Considered Sites Site: MICHIGAN [VELSICOL] CHEMICAL CORP. (MI.03 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Velsicol Chemical Corp. MI.03-1 Location: St. Louis , Michigan MI.03-2 Evaluation Year: Circa 1987 MI.03-3 Site Operations: Rare earth processing facility. MI.03-2 Site Disposition: Eliminated - No Authority - NRC survey MI.03-3 Radioactive Materials Handled: Yes Primary Radioactive

  13. DOE - Office of Legacy Management -- Star Cutter Corp - MI 15

    Office of Legacy Management (LM)

    Star Cutter Corp - MI 15 FUSRAP Considered Sites Site: STAR CUTTER CORP. (MI.15) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Farmington , Michigan MI.15-1 Evaluation Year: 1991 MI.15-2 Site Operations: Performed a one time uranium slug drilling operation test in 1956. MI.15-3 MI.15-1 Site Disposition: Eliminated - Potential for contamination considered remote based on limited scope and quantity of materials handled MI.15-2 Radioactive

  14. File:USDA-CE-Production-GIFmaps-NY.pdf | Open Energy Information

    Open Energy Info (EERE)

    NY.pdf Jump to: navigation, search File File history File usage New York Ethanol Plant Locations Size of this preview: 776 600 pixels. Full resolution (1,650 1,275 pixels,...

  15. BuildSmart NY Innovators Summit Offers Sneak Peek at Better Buildings

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Challenge Solutions to Come | Department of Energy BuildSmart NY Innovators Summit Offers Sneak Peek at Better Buildings Challenge Solutions to Come BuildSmart NY Innovators Summit Offers Sneak Peek at Better Buildings Challenge Solutions to Come September 18, 2014 - 12:32pm Addthis The graphic above highlights New York Power Authority’s energy efficiency goals. | Courtesy of New York Power Authority The graphic above highlights New York Power Authority's energy efficiency goals. |

  16. DOE - Office of Legacy Management -- Pfaltz and Bauer Inc - New York - NY

    Office of Legacy Management (LM)

    45 New York - NY 45 FUSRAP Considered Sites Site: Pfaltz and Bauer Inc - New York (NY.45) Designated Name: Alternate Name: Location: Evaluation Year: Site Operations: Site Disposition: Radioactive Materials Handled: Primary Radioactive Materials Handled: Radiological Survey(s): Site Status: This site is one of a group of 77 FUSRAP considered sites for which few, if any records are available in their respective site files to provide an historical account of past operations and their

  17. DOE - Office of Legacy Management -- Pier 38 - NY 0-18

    Office of Legacy Management (LM)

    Pier 38 - NY 0-18 FUSRAP Considered Sites Site: Pier 38 (NY.0-18 ) Designated Name: Alternate Name: Location: Evaluation Year: Site Operations: Site Disposition: Radioactive Materials Handled: Primary Radioactive Materials Handled: Radiological Survey(s): Site Status: This site is one of a group of 77 FUSRAP considered sites for which few, if any records are available in their respective site files to provide an historical account of past operations and their relationship, if any, with MED/AEC

  18. DOE - Office of Legacy Management -- West Milton Reactor Site - NY 21

    Office of Legacy Management (LM)

    Milton Reactor Site - NY 21 FUSRAP Considered Sites Site: West Milton Reactor Site (NY.21) Designated Name: Alternate Name: Location: Evaluation Year: Site Operations: Site Disposition: Radioactive Materials Handled: Primary Radioactive Materials Handled: Radiological Survey(s): Site Status: This site is one of a group of 77 FUSRAP considered sites for which few, if any records are available in their respective site files to provide an historical account of past operations and their

  19. U.S. Natural Gas Exports to Mexico

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    MI St. Clair, MI Noyes, MN Warroad, MN Babb, MT Havre, MT Port of Morgan, MT Sherwood, ND Pittsburg, NH Buffalo, NY Grand Island, NY Massena, NY Niagara Falls, NY Waddington, NY...

  20. DOE - Office of Legacy Management -- Wolverine Tube Division - MI 05

    Office of Legacy Management (LM)

    Wolverine Tube Division - MI 05 FUSRAP Considered Sites Site: Wolverine Tube Division (MI.05) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Wolverine Tube Division of Calumet & Hecla Consolidated Copper Co. Star Tool Hermes Automotive Manufacturing Corporation MI.05-1 MI.05-2 Location: 1411 Central Avenue , Detroit , Michigan MI.05-3 Evaluation Year: 1990 MI.05-2 Site Operations: 1943 - Conducted research and development of methods for spinning

  1. DOE - Office of Legacy Management -- Adrian - MI 01

    Office of Legacy Management (LM)

    Adrian - MI 01 FUSRAP Considered Sites Adrian, MI Alternate Name(s): Bridgeport Brass Co. Special Metals Extrusion Plant Bridgeport Brass Company General Motors General Motors Company, Adrian MI.01-1 Location: 1450 East Beecher Street, Adrian, Michigan MI.01-3 Historical Operations: Performed uranium extrusion research and development and metal fabrication work for the AEC using uranium, thorium, and plutonium. MI.01-2 Eligibility Determination: Eligible MI.01-1 Radiological Survey(s):

  2. File:USDA-CE-Production-GIFmaps-NC.pdf | Open Energy Information

    Open Energy Info (EERE)

    NC.pdf Jump to: navigation, search File File history File usage North Carolina Ethanol Plant Locations Size of this preview: 776 600 pixels. Full resolution (1,650 1,275...

  3. MiniBooNE NC 1?0 Cross Section Data Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    0 production cross sections on mineral oil at EO(1 GeV)", arXiv:0911.2063 hep-ex, Phys. Rev. D81, 013005 (2010) The following MiniBooNE information from the 2009 NC 10...

  4. DOE - Office of Legacy Management -- Carboloy Co - MI 12

    Office of Legacy Management (LM)

    Carboloy Co - MI 12 FUSRAP Considered Sites Site: Carboloy Co. (MI.12 ) Eliminated from further consideration under FUSRAP - AEC licensed facility Designated Name: Not Designated Alternate Name: General Electric MI.12-1 Location: 11177 E. Eight Mile Road , Detroit , Michigan MI.12-1 MI.12-2 Evaluation Year: 1987-1991 MI.12-3 MI.12-4 MI.12-6 Site Operations: Turned-down the outer diameter of uranium metal slugs and conducted pilot plant scale operations for hot pressing uranium dioxide pellets

  5. The baryon vector current in the combined chiral and 1/Nc expansions

    SciTech Connect (OSTI)

    Flores-Mendieta, Ruben; Goity, Jose L

    2014-12-01

    The baryon vector current is computed at one-loop order in large-Nc baryon chiral perturbation theory, where Nc is the number of colors. Loop graphs with octet and decuplet intermediate states are systematically incorporated into the analysis and the effects of the decuplet-octet mass difference and SU(3) flavor symmetry breaking are accounted for. There are large-Nc cancellations between different one-loop graphs as a consequence of the large-Nc spin-flavor symmetry of QCD baryons. The results are compared against the available experimental data through several fits in order to extract information about the unknown parameters. The large-Nc baryon chiral perturbation theory predictions are in very good agreement both with the expectations from the 1/Nc expansion and with the experimental data. The effect of SU(3) flavor symmetry breaking for the |Delta S|=1 vector current form factors f1(0) results in a reduction by a few percent with respect to the corresponding SU(3) symmetric values.

  6. Buffalo, NY Liquefied Natural Gas Exports to Canada (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    to Canada (Million Cubic Feet) Buffalo, NY Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2015 1 1 1 1 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Exports by Point of Exit Buffalo, NY Liquefied Natural Gas Exports to Canada

  7. Champlain, NY Natural Gas Pipeline Imports From Canada (Million Cubic Feet)

    Gasoline and Diesel Fuel Update (EIA)

    Feet) (Million Cubic Feet) Champlain, NY Natural Gas Liquefied Natural Gas Imports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2014 63 2015 1 2 1 2 20 2016 56 76 20 20 3 4 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Imports by Point of Entry Champlain, NY LNG Imports from All Countries

  8. DOE - Office of Legacy Management -- Oliver Corp - MI 11

    Office of Legacy Management (LM)

    Oliver Corp - MI 11 FUSRAP Considered Sites Site: OLIVER CORP. (MI.11 ) Eliminated from further consideration under FUSRAP - Referred to NRC Designated Name: Not Designated Alternate Name: Behnke Warehousing Incorporated MI.11-1 Location: 433 East Michigan Avenue , Battle Creek , Michigan MI.11-1 Evaluation Year: 1986 MI.11-4 Site Operations: Conducted production scale briquetting of green salt and magnesium blend under AEC license Nos. SNM-591, SUB-579, and C-3725. MI.11-1 MI.11-3 Site

  9. The NuMI Neutrino Beam

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Adamson, P.; Anderson, K.; Andrews, M.; Andrews, R.; Anghel, I.; Augustine, D.; Aurisano, A.; Avvakumov, S.; Ayres, D. S.; Baller, B.; et al

    2015-10-20

    Our paper describes the hardware and operations of the Neutrinos at the Main Injector (NuMI) beam at Fermilab. It elaborates on the design considerations for the beam as a whole and for individual elements. The most important part of our design details pertaining to individual components is described. Beam monitoring systems and procedures, including the tuning and alignment of the beam and NuMI long-term performance, are also discussed.

  10. DOE - Office of Legacy Management -- Detrex Corp - MI 10

    Office of Legacy Management (LM)

    Detrex Corp - MI 10 FUSRAP Considered Sites Site: Detrex Corp. (MI.10 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Detroit , Michigan MI.10-1 Evaluation Year: 1987 MI.10-2 Site Operations: Conducted experimental runs relative to pickling/degreasing of one handful of uranium turnings MI.10-1 Site Disposition: Eliminated - Potential for contamination considered remote due to small quantity of material handled - There is no

  11. DOE Zero Energy Ready Home Case Study: Greenhill Contracting, New Paltz, NY

    Broader source: Energy.gov [DOE]

    Case study of a DOE Zero Energy Ready home in New Paltz, NY, that achieved a HERS score of 37 without PV or 7 with 7.5-kW PV. The two-story 2,288-ft2 home is one of 9 certified homes. All of the...

  12. DOE Zero Energy Ready Home Case Study: AquaZephyr, Ithaca, NY

    Broader source: Energy.gov [DOE]

    Case study of a DOE Zero Energy Ready home in Ithaca, NY, that achieves a HERS 56 without PV or HERS 15 with 4-kW of PV. The two-story, 1,664-ft2 home is one of 17 single-family and 4 duplex homes...

  13. DOE Zero Energy Ready Home Case Study: AquaZephyr, Ithaca, NY...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Case study of a DOE Zero Energy Ready home in Ithaca, NY, that achieves a HERS 56 without PV or HERS 15 with 4-kW of PV. The two-story, 1,664-ft2 home is one of 17 single-family ...

  14. SGDP Report: Interoperability of Demand Response Resources Demonstration in NY (February 2015)

    Office of Energy Efficiency and Renewable Energy (EERE)

    The Interoperability of Demand Response Resources Demonstration in NY was awarded to Con Edison in 2009 as part of DOE's Smart Grid Demonstration Project (SGDP) grants funded by the Recovery Act. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited demand response resources, both conventional and renewable, to integrate more effectively with electric delivery companies.

  15. SGDP Report Now Available: Interoperability of Demand Response Resources Demonstration in NY (February 2015)

    Broader source: Energy.gov [DOE]

    The Interoperability of Demand Response Resources Demonstration in NY was awarded to Con Edison in 2009 as part of DOE's Smart Grid Demonstration Project (SGDP) grants funded by the Recovery Act. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited demand response resources, both conventional and renewable, to integrate more effectively with electric delivery companies

  16. North Carolina School of Science and Mathematics from Durham, NC and

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Albuquerque Academy from Albuquerque, NM Win the U.S. Department of Energy National Science Bowl | Department of Energy Carolina School of Science and Mathematics from Durham, NC and Albuquerque Academy from Albuquerque, NM Win the U.S. Department of Energy National Science Bowl North Carolina School of Science and Mathematics from Durham, NC and Albuquerque Academy from Albuquerque, NM Win the U.S. Department of Energy National Science Bowl May 3, 2010 - 12:00am Addthis WASHINGTON, D.C. - A

  17. F-1 U.S. Energy Information Administration | Annual Energy Outlook...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Central West North Central East North Central Mountain AK WA MT WY ID NV UT CO AZ NM TX OK IA KS MO IL IN KY TN MS AL FL GA SC NC WV PA NJ MD DE NY CT VT ME RI MA NH VA WI MI OH...

  18. F-5 U.S. Energy Information Administration | Annual Energy Outlook...

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Figure F4. Oil and Gas Supply Model Regions Atlantic WA MT WY ID NV UT CO AZ NM TX OK IA KS MO IL IN KY TN MS AL FL GA SC NC WV PA NJ MD DE NY CT ME RI MA NH VA WI MI OH NE...

  19. Chapter V

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... AL LA GA AR NC NY MI PA IN VA MS TN KY OH ME SC WV MD VT MA NH CT NJ DE R I DC b Coal Fired Power Plant Supplied by the Powder River Basin Powder River Basin 0 220 110 Miles The ...

  20. Numerical study of self modulation instability of 1 nC electron bunch at ATF

    SciTech Connect (OSTI)

    Fang Yun; Mori, Warren; Muggli, Patric

    2012-12-21

    The development of self-modulation instability (SMI) is investigated numerically for the 1 nC electron bunch available at Accelerator Test Facility (ATF) of Brookhaven National Laboratory (BNL). Possible experiment based on the simulation results is proposed. All the simulations are performed with the 2D-cylindrically symmetric particle-in-cell code.

  1. DOE - Office of Legacy Management -- Naval Ordnance Plant - MI...

    Office of Legacy Management (LM)

    Eliminated from further consideration under FUSRAP - Referred to DoD for action Designated ... MI.0-03-1 Site Disposition: Eliminated - No Authority - Referred to DoD MI.0-03-1 ...

  2. DOE Zero Energy Ready Home Case Study: Ferguson Design and Construction Inc., Sagaponack, NY, Custom Home

    Broader source: Energy.gov [DOE]

    Case study of a DOE Zero Energy Ready Home in Long Island, NY, that scored HERS 43 without PV. This 5,088-square-foot custom home has R-25 double-stud walls, a vaulted roof with R-40 blown cellulose, R-10 XPS under slab, a hydro air system with 91% efficient boiler for forced air and radiant floor heat, and 100% LED lights.

  3. DOE Zero Energy Ready Home Case Study 2013: Ferguson Design and Construction, Inc., Sagaponak, NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ferguson Design & Construction, Inc. Sagaponack, NY BUILDING TECHNOLOGIES OFFICE The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specifi ed in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy ReadyHome starts with ENERGY STAR for Homes Version 3 for an energy-effi cient home built on a solid foundation of building science research. Advanced technologies are designed

  4. DOE Zero Energy Ready Home Case Study 2013: Ithaca Neighborhood Housing Services, Ithaca, NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Ithaca Neighborhood Housing Services Ithaca, NY BUILDING TECHNOLOGIES OFFICE The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specifi ed in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR for Homes Version 3 for an energy-effi cient home built on a solid foundation of building science research. Advanced technologies are designed in to

  5. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor

    SciTech Connect (OSTI)

    Park, Jong-Kook; Henry, Jon C.; Jiang, Jinmai; Esau, Christine; Gusev, Yuriy; Lerner, Megan R.; Postier, Russell G.; Brackett, Daniel J.; Schmittgen, Thomas D.

    2011-03-25

    Research highlights: {yields} The expression of miR-132 and miR-212 are significantly increased in pancreatic cancer. {yields} miR-132 and miR-212 target the tumor suppressor pRb, resulting in enhanced proliferation. {yields} miR-132 and miR-212 expression is increased by a {beta}2 adrenergic receptor agonist, suggesting a novel mechanism for pancreatic cancer progression. -- Abstract: Numerous microRNAs (miRNAs) are reported as differentially expressed in cancer, however the consequence of miRNA deregulation in cancer is unknown for many miRNAs. We report that two miRNAs located on chromosome 17p13, miR-132 and miR-212, are over-expressed in pancreatic adenocarcinoma (PDAC) tissues. Both miRNAs are predicted to target the retinoblastoma tumor suppressor, Rb1. Validation of this interaction was confirmed by luciferase reporter assay and western blot in a pancreatic cancer cell line transfected with pre-miR-212 and pre-miR-132 oligos. Cell proliferation was enhanced in Panc-1 cells transfected with pre-miR-132/-212 oligos. Conversely, antisense oligos to miR-132/-212 reduced cell proliferation and caused a G{sub 2}/M cell cycle arrest. The mRNA of a number of E2F transcriptional targets were increased in cells over expressing miR-132/-212. Exposing Panc-1 cells to the {beta}2 adrenergic receptor agonist, terbutaline, increased the miR-132 and miR-212 expression by 2- to 4-fold. We report that over-expression of miR-132 and miR-212 result in reduced pRb protein in pancreatic cancer cells and that the increase in cell proliferation from over-expression of these miRNAs is likely due to increased expression of several E2F target genes. The {beta}2 adrenergic pathway may play an important role in this novel mechanism.

  6. “Nodal Gap” induced by the incommensurate diagonal spin density modulation in underdoped high- <mi>Tmi>c> superconductors

    SciTech Connect (OSTI)

    Zhou, Tao; Gao, Yi; Zhu, Jian -Xin

    2015-03-07

    Recently it was revealed that the whole Fermi surface is fully gapped for several families of underdoped cuprates. The existence of the finite energy gap along the <mi>d>-wave nodal lines (nodal gap) contrasts the common understanding of the <mi>d>-wave pairing symmetry, which challenges the present theories for the high-<mi>Tmi><mi>c>superconductors. Here we propose that the incommensurate diagonal spin-density-wave order can account for the above experimental observation. The Fermi surface and the local density of states are also studied. Our results are in good agreement with many important experiments in high-<mi>Tmi><mi>c>superconductors.

  7. Characterization of function and regulation of miR-24-1 and miR-31

    SciTech Connect (OSTI)

    Sun Fenyong; Wang Jiayi; Pan Qiuhui; Yu Yongchun; Zhang Yue; Wan Yang; Wang Ju; Li Xiaoyan; Hong An

    2009-03-13

    To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b{approx}27b{approx}24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together.

  8. MiniBooNE NC 1π0 Cross Section Data Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    νμ and ν̅μ induced neutral current single π0 production cross sections on mineral oil at Eν~O(1 GeV)", arXiv:0911.2063 [hep-ex], Phys. Rev. D81, 013005 (2010) The following MiniBooNE information from the 2009 NC 1π0 cross section paper is made available to the public: Neutrino Mode Running νμ NC 1π0 pπ0 Differential Cross Section 1D array of bin boundaries partitioning the momentum of the π0 1D array of the value of the differential cross section in each bin in units of 10-40

  9. DOE - Office of Legacy Management -- Dow Chemical Co - Midland - MI 06

    Office of Legacy Management (LM)

    Midland - MI 06 FUSRAP Considered Sites Site: Dow Chemical Co. - Midland (MI.06 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Midland , Michigan MI.06-1 Evaluation Year: Circa 1987 MI.06-2 Site Operations: Conducted development work for production of magnesium-thorium alloys. MI.06-1 Site Disposition: Eliminated - AEC licensed site MI.06-1 MI.06-2 Radioactive Materials Handled: Yes Primary Radioactive Materials Handled:

  10. Nitrogen effects on crystallization kinetics of amorphous TiOxNy thin films

    SciTech Connect (OSTI)

    Hukari, Kyle; Dannenberg, Rand; Stach, E.A.

    2001-03-30

    The crystallization behavior of amorphous TiOxNy (x>>y) thin films was investigated by in-situ transmission electron microscopy. The Johnson-Mehl-Avrami-Kozolog (JMAK) theory is used to determine the Avrami exponent, activation energy, and the phase velocity pre-exponent. Addition of nitrogen inhibits diffusion, increasing the nucleation temperature, while decreasing the growth activation energy. Kinetic variables extracted from individual crystallites are compared to JMAK analysis of the fraction transformed and a change of 6 percent in the activation energy gives agreement between the methods. From diffraction patterns and index of refraction the crystallized phase was found to be predominantly anatase.

  11. DOE Zero Ready Home Case Study: AquaZephyr, Eco-Vilage Ithaca, Ithaca NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    AquaZephyr Eco-Village Ithaca Ithaca, NY DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced technologies are designed in to

  12. DOE Zero Ready Home Case Study: Greenhill Contracting, The Preserve, New Paltz, NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    The Preserve New Paltz, NY DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced technologies are designed in to give you

  13. DOE - Office of Legacy Management -- General Motors Co - Flint - MI 07

    Office of Legacy Management (LM)

    Motors Co - Flint - MI 07 FUSRAP Considered Sites Site: GENERAL MOTORS CO. (MI.07 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: A.C. Spark Plug Dort Highway Plant MI.07-1 MI.07-2 Location: Flint , Michigan MI.07-1 Evaluation Year: 1987 MI.07-3 Site Operations: Processed thorium oxide, uranium oxide, and beryllium oxide into crucibles for the Chicago Area. MI.07-3 MI.07-4 MI.07-5 Site Disposition: Eliminated - Potential for contamination

  14. MINOS Experiment and NuMI Beam Home Page

    Broader source: All U.S. Department of Energy (DOE) Office Webpages

    NuMI-MINOS Neutrino Logo NuMI Beamline and MINOS Experiment Neutrino Logo The MINOS Experiment and NuMI Beamline Fermilab Logo MINOS Experiment Links ◊ MINOS for the Public ◊ Scientific Results ◊ MINOS at Work ◊ NuMI at Work ◊ MINOS+ Experiment Fermilab Neutrino Links ◊ Neutrino FAQ ◊ MINOS Underground Areas at Fermilab ◊ PPD Intensity Frontier Dept Back to - - - ◊ Fermilab at Work ◊ Fermilab Home the MINOS Far Detector in the Soudan Mine MINOS collaborators assembling the

  15. Magnetocrystalline anisotropy in <mi>UMn>2<mi>Ge>2 and related Mn-based actinide ferromagnets

    SciTech Connect (OSTI)

    Parker, David S.; Ghimire, Nirmal; Singleton, John; Thompson, J. D.; Bauer, Eric D.; Baumbach, Ryan; Mandrus, David; Li, Ling; Singh, David J.

    2015-05-04

    We present magnetization isotherms in pulsed magnetic fields up to 62 Tesla, supported by first principles calculations, demonstrating a huge uniaxial magnetocrystalline anisotropy energy - approximately 20 MJ/m3 - in <mi>UMn>2<mi>Ge>2. This large anisotropy results from the extremely strong spin-orbit coupling affecting the uranium 5 f electrons, which in the calculations exhibit a substantial orbital moment exceeding 2 μB. Finally, we also find from theoretical calculations that a number of isostructural Mn-actinide compounds are expected to have similarly large anisotropy.

  16. ,"Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf...

    U.S. Energy Information Administration (EIA) Indexed Site

    ,"Worksheet Name","Description"," Of Series","Frequency","Latest Data for" ,"Data 1","Detroit, MI Natural Gas Pipeline Imports From Canada (MMcf)",1,"Annual",2014 ,"Release...

  17. The origin and fate of the sediments composing a migrating dune field, Amagansett, NY

    SciTech Connect (OSTI)

    Maher, T. (Suffolk Community Coll., Selden, NY (United States). Environmental Science Dept.); Kandelin, J. (Suffolk Community Coll., Selden, NY (United States). Dept. of Earth and Space Science); Black, J.A. (Geosciences Inc., Patchogue, NY (United States))

    1993-03-01

    The migrating dune system, located in Amagansett, NY, consists of a series of three parabolic dunes ranging in heights from 10 to 30 meters. The dunes are migrating under the influence of the prevailing winds, in a southeasterly direction. The migration continues until the dunes encounter the countervailing prevailing winds, off the Atlantic Ocean. A series of flow charts have been prepared to indicate the possible sources of sediment for this system. These charts, in conjunction with geomorphic analysis, stratigraphic data and various sediment characteristics indicate that the sediments are transported by coastal currents. Once deposited they form a linear dune system. Eolian transport from this dune then supplies the sediment to the migrating dune system.

  18. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    SciTech Connect (OSTI)

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation of the

  19. miR-92a family and their target genes in tumorigenesis and metastasis

    SciTech Connect (OSTI)

    Li, Molin; Guan, Xingfang; Sun, Yuqiang; Mi, Jun; Shu, Xiaohong; Liu, Fang; Li, Chuangang

    2014-04-15

    The miR-92a family, including miR-25, miR-92a-1, miR-92a-2 and miR-363, arises from three different paralog clusters miR-17-92, miR-106a-363, and miR-106b-25 that are highly conservative in the process of evolution, and it was thought as a group of microRNAs (miRNAs) correlated with endothelial cells. Aberrant expression of miR-92a family was detected in multiple cancers, and the disturbance of miR-92a family was related with tumorigenesis and tumor development. In this review, the progress on the relationship between miR-92a family and their target genes and malignant tumors will be summarized. - Highlights: Aberrant expression of miR-92a, miR-25 and miR-363 can be observed in many kinds of malignant tumors. The expression of miR-92a family is regulated by LOH, epigenetic alteration, transcriptional factors such as SP1, MYC, E2F, wild-type p53 etc. Roles of miR-92a family in tumorigenesis and development: promoting cell proliferation, invasion and metastasis, inhibiting cell apoptosis.

  20. The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex

    SciTech Connect (OSTI)

    Blanc, Guillaume; Duncan, Garry A.; Agarakova, Irina; Borodovsky, Mark; Gurnon, James; Kuo, Alan; Lindquist, Erika; Lucas, Susan; Pangailinan, Jasmyn; Polle, Juergen; Salamov, Asaf; Terry, Astrid; Yamada, Takashi; Dunigan, David D.; Grigoriev, Igor V.; Claverie, Jean-Michel; Etten, James L. Van

    2010-05-06

    Chlorella variabilis NC64A, a unicellular photosynthetic green alga (Trebouxiophyceae), is an intracellular photobiont of Paramecium bursaria and a model system for studying virus/algal interactions. We sequenced its 46-Mb nuclear genome, revealing an expansion of protein families that could have participated in adaptation to symbiosis. NC64A exhibits variations in GC content across its genome that correlate with global expression level, average intron size, and codon usage bias. Although Chlorella species have been assumed to be asexual and nonmotile, the NC64A genome encodes all the known meiosis-specific proteins and a subset of proteins found in flagella. We hypothesize that Chlorella might have retained a flagella-derived structure that could be involved in sexual reproduction. Furthermore, a survey of phytohormone pathways in chlorophyte algae identified algal orthologs of Arabidopsis thaliana genes involved in hormone biosynthesis and signaling, suggesting that these functions were established prior to the evolution of land plants. We show that the ability of Chlorella to produce chitinous cell walls likely resulted from the capture of metabolic genes by horizontal gene transfer from algal viruses, prokaryotes, or fungi. Analysis of the NC64A genome substantially advances our understanding of the green lineage evolution, including the genomic interplay with viruses and symbiosis between eukaryotes.

  1. DOE - Office of Legacy Management -- Baker-Perkins Co - MI 13

    Office of Legacy Management (LM)

    Baker-Perkins Co - MI 13 FUSRAP Considered Sites Site: Baker-Perkins Co (MI 13) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Saginaw , Michigan MI.13-1 Evaluation Year: 1991 MI.13-1 MI.13-2 Site Operations: Small scale oxide mixing demonstrations and testing in May, 1956. MI.13-2 Site Disposition: Eliminated - Potential for contamination remote based on limited scope of activities at the site MI.13-3 Radioactive Materials Handled: Yes

  2. DOE - Office of Legacy Management -- Revere Copper and Brass Co - MI 04

    Office of Legacy Management (LM)

    Revere Copper and Brass Co - MI 04 FUSRAP Considered Sites Site: REVERE COPPER AND BRASS CO. ( MI.04 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Revere Copper and Brass MI.04-1 Location: 5851 West Jefferson Street , Detroit , Michigan MI.04-1 Evaluation Year: 1990 MI.04-2 Site Operations: Extrusion of tuballoy rods, myrnalloy rods and beryllium shapes in the 1940s. MI.04-3 MI.04-4 Site Disposition: Eliminated - Radiation levels below criteria

  3. HIA 2015 DOE Zero Energy Ready Home Case Study: United Way of Long Island Housing Development Corporation, Patchogue, NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Way of Long Island Housing Development Corporation Patchogue, NY DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced

  4. Origin State>> CA ID ID ID IL MD NM NM NY OH TN TN TN, WA, CA

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NY OH TN TN TN, WA, CA TN TN TN TX Total Shipments by Route Lawrence Livermore National Laboratory Batelle Energy Alliance Idaho National Laboratory Advanced Mixed Waste Treatment Project Argonne National Laboratory Aberdeen Proving Ground Sandia National Laboratory Los Alamos National Laboratory Brookhaven National Laboratory Portsmouth Gaseous Diffusion Plant Duratek/Energy Solutions Babcox & Wilcox Technical Services Y-12 Plant Materials & Energy Corporation (M&EC) Perma-Fix

  5. Origin State>> CA ID ID IL IL KY NM NM NV NY OH TN TN TN, WA,

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    IL IL KY NM NM NV NY OH TN TN TN, WA, CA TN TN TN TN Total Shipments by Route Lawrence Livermore National Laboratory Batelle Energy Alliance Idaho National Laboratory Energx Argonne National Laboratory Argonne National Laboratory Paducah Gaseous Diffusion Plant Sandia National Laboratory Los Alamos National Laboratory National Security Technologies West Valley Environmental Services Portsmouth Gaseous Diffusion Plant Duratek/Energy Solutions Babcox & Wilcox Technical Services Y-12 Plant

  6. miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression

    SciTech Connect (OSTI)

    Gao, Yong; Luo, Ling-hui; Li, Shuai; Yang, Cao

    2014-02-07

    Highlights: • miR-17 was increased in OS tissues and cell lines. • Inhibition of miR-17 suppressed OS cell proliferation. • Inhibition of miR-17 suppressed OS cell migration and invasion. • PTEN was a target of miR-17. • miR-17 was negatively correlated with PTEN in OS tissues. - Abstract: MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3′-untranslated region (3′-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.

  7. MiR-218 Mediates tumorigenesis and metastasis: Perspectives and implications

    SciTech Connect (OSTI)

    Lu, Ying-fei; Zhang, Li; Waye, Mary Miu Yee; Fu, Wei-ming; Zhang, Jin-fang

    2015-05-15

    MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. As a highly conserved miRNA across a variety of species, microRNA-218 (miR-218) was found to play pivotal roles in tumorigenesis and progression. A group of evidence has demonstrated that miR-218 acts as a tumor suppressor by targeting many oncogenes related to proliferation, apoptosis and invasion. In this review, we provide a complex overview of miR-218, including its regulatory mechanisms, known functions in cancer and future challenges as a potential therapeutic target in human cancers. - Highlights: • miR-218 is frequently down regulated in multiple cancers. • miR-218 plays pivotal roles in carcinogenesis. • miR-218 mediates proliferation, apoptosis, metastasis, invasion, etc. • miR-218 mediates tumorigenesis and metastasis via multiple pathways.

  8. Superscaling in electron-nucleus scattering and its link to CC and NC QE neutrino-nucleus scattering

    SciTech Connect (OSTI)

    Barbaro, M. B.; Amaro, J. E.; Caballero, J. A.; González-Jiménez, R.; Donnelly, T. W.; Ivanov, M.; Udías, J. M.

    2015-05-15

    The superscaling approach (SuSA) to neutrino-nucleus scattering, based on the assumed universality of the scaling function for electromagnetic and weak interactions, is reviewed. The predictions of the SuSA model for bot CC and NC differential and total cross sections are presented and compared with the MiniBooNE data. The role of scaling violations, in particular the contribution of meson exchange currents in the two-particle two-hole sector, is explored.

  9. Category:Utility Rate Impacts on PV Economics By Location | Open...

    Open Energy Info (EERE)

    MI Traverse City, MI International Falls, MN Minneapolis, MN Kansas City, MO Jackson, MS Billings, MT Greensboro, NC Wilmington, NC Bismarck, ND Minot, ND Omaha, NE...

  10. DOE - Office of Legacy Management -- Dow-Detroit Edison Project - MI 0-02

    Office of Legacy Management (LM)

    Dow-Detroit Edison Project - MI 0-02 FUSRAP Considered Sites Site: Dow-Detroit Edison Project (MI.0-02 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Detroit , Michigan MI.0-02-1 Evaluation Year: 1987 MI.0-02-1 Site Operations: Performed reference design work for a special fast breeder type reactor. MI.0-02-1 Site Disposition: Eliminated - No radioactive material handled at the site MI.0-02-1 Radioactive Materials Handled: No

  11. DOE - Office of Legacy Management -- Mitts-Merrill Co - MI 14

    Office of Legacy Management (LM)

    Mitts-Merrill Co - MI 14 FUSRAP Considered Sites Site: MITTS and MERRILL CO. (MI.14 ) Eliminated from consideration under FUSRAP Designated Name: Not Designated Alternate Name: Genessee Packing Co. MI.14-1 Location: Saginaw, Michigan MI.14-1 Evaluation Year: 1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces on a small test scale during the mid-1950s. MI.14-1 Site Disposition: Eliminated - Potential for contamination considered remote based on limited quantity

  12. miRNA-205 affects infiltration and metastasis of breast cancer

    SciTech Connect (OSTI)

    Wang, Zhouquan; Department of Tumor, SenGong Hospital of Shaanxi, Xian 710300 ; Liao, Hehe; Deng, Zhiping; Yang, Po; Du, Ning; Zhanng, Yunfeng; Ren, Hong

    2013-11-08

    Highlights: We detected expression of miR-205 in breast cancer cell lines and tissue samples. We suggest miR-205 is downregulated in human breast cancer tissues and MCF7 cells. We suggest the lower expression of miR-205 play a role in breast cancer onset. These data suggest that miR-205 directly targets HER3 in human breast cancer. -- Abstract: Background: An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy. Methods: The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. Results: miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3? untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression. Conclusion: miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.

  13. HIA 2015 DOE Zero Energy Ready Home Case Study: Greenhill Contracting, Green Acres #20, #26, #28, New Paltz, NY

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Green Acres #20, #26, #28 New Paltz, NY DOE ZERO ENERGY READY HOME(tm) The U.S. Department of Energy invites home builders across the country to meet the extraordinary levels of excellence and quality specified in DOE's Zero Energy Ready Home program (formerly known as Challenge Home). Every DOE Zero Energy Ready Home starts with ENERGY STAR Certified Homes Version 3.0 for an energy-efficient home built on a solid foundation of building science research. Advanced technologies are designed in to

  14. NuMI Low Energy Flux Prediction Release

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    NuMI Low Energy Flux Prediction Release Neutrino Flux Predictions for the NuMI Beam hep-ex/1607.00704 Data Ancillary data files for this result are available on arXiv at http://arxiv.org/src/1607.00704/anc.< /li> Among the available data files are: pdf file describing format of all the available files root file of all the available fluxes python code to read and process MINERvA's flux predictions Text Files of the flux, uncertainties, and covariance matrix, with units of neutrinos/m^2/POT,

  15. Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 1 2014 1 1 1 1 2 1 1 1 1 1 2015 1 1 1 1 1 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Exports by Point of Exit Port Huron, MI LNG Exports to All Countries

  16. Ground Motion Studies at NuMI

    SciTech Connect (OSTI)

    Mayda M. Velasco; Michal Szleper

    2012-02-20

    Ground motion can cause significant deterioration in the luminosity of a linear collider. Vibration of numerous focusing magnets causes continuous misalignments, which makes the beam emittance grow. For this reason, understanding the seismic vibration of all potential LC sites is essential and related efforts in many sites are ongoing. In this document we summarize the results from the studies specific to Fermilab grounds as requested by the LC project leader at FNAL, Shekhar Mishra in FY04-FY06. The Northwestern group focused on how the ground motion effects vary with depth. Knowledge of depth dependence of the seismic activity is needed in order to decide how deep the LC tunnel should be at sites like Fermilab. The measurements were made in the NuMI tunnel, see Figure 1. We take advantage of the fact that from the beginning to the end of the tunnel there is a height difference of about 350 ft and that there are about five different types of dolomite layers. The support received allowed to pay for three months of salary of Michal Szleper. During this period he worked a 100% of his time in this project. That include one week of preparation: 2.5 months of data taking and data analysis during the full period of the project in order to guarantee that we were recording high quality data. We extended our previous work and made more systematic measurements, which included detailed studies on stability of the vibration amplitudes at different depths over long periods of time. As a consequence, a better control and more efficient averaging out of the daytime variation effects were possible, and a better study of other time dependences before the actual depth dependence was obtained. Those initial measurements were made at the surface and are summarized in Figure 2. All measurements are made with equipment that we already had (two broadband seismometers KS200 from GEOTECH and DL-24 portable data recorder). The offline data analysis took advantage of the full Fourier spectra

  17. DOE - Office of Legacy Management -- Mitts-Merrel Co - MI 14

    Office of Legacy Management (LM)

    1993 MI.14-2 Site Operations: Reduced thorium metal chunks into particle sized pieces ... Primary Radioactive Materials Handled: Thorium MI.14-1 Radiological Survey(s): Yes - ...

  18. MiR-125a TNF receptor-associated factor 6 to inhibit osteoclastogenesis

    SciTech Connect (OSTI)

    Guo, Li-Juan; Liao, Lan; Yang, Li; Li, Yu; Jiang, Tie-Jian

    2014-02-15

    MicroRNAs (miRNAs) play important roles in osteoclastogenesis and bone resorption. In the present study, we found that miR-125a was dramatically down-regulated during macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-κB ligand (RANKL) induced osteoclastogenesis of circulating CD14+ peripheral blood mononuclear cells (PBMCs). Overexpression of miR-125a in CD14+ PBMCs inhibited osteoclastogenesis, while inhibition of miR-125a promoted osteoclastogenesis. TNF receptor-associated factor 6 (TRAF6), a transduction factor for RANKL/RANK/NFATc1 signal, was confirmed to be a target of miR-125a. EMSA and ChIP assays confirmed that NFATc1 bound to the promoter of the miR-125a. Overexpression of NFATc1 inhibited miR-125a transcription, and block of NFATc1 expression attenuated RANKL-regulated miR-125a transcription. Here, we reported that miR-125a played a biological function in osteoclastogenesis through a novel TRAF6/ NFATc1/miR-125a regulatory feedback loop. It suggests that regulation of miR-125a expression may be a potential strategy for ameliorating metabolic disease. - Highlights: • MiR-125a was significantly down-regulated in osteoclastogenesis of CD14+ PBMCs. • MiR-125a inhibited osteoclast differentiation by targeting TRAF6. • NFATc1 inhibited miR-125a transciption by binding to the promoter of miR-125a. • TRAF6/NFATc1 and miR-125a form a regulatory feedback loop in osteoclastogenesis.

  19. DOE - Office of Legacy Management -- Amex Specialty Metal Corp - MI 0-01

    Office of Legacy Management (LM)

    Amex Specialty Metal Corp - MI 0-01 FUSRAP Considered Sites Site: Amex Specialty Metal Corp (MI.0-01 ) Eliminated from further consideration under FUSRAP Designated Name: Not Designated Alternate Name: None Location: Coldwater , Michigan MI.0-01-1 Evaluation Year: 1987 MI.0-01-1 Site Operations: No indication that AMEX performed work for MED or AEC activities. Originally included on FUSRAP list due to fact that AMEX purchased milling equipment from a company that had done uranium milling.

  20. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma

    SciTech Connect (OSTI)

    Xiong, Xinkui; Sun, Daoyi; Chai, Hao; Shan, Wengang; Yu, Yue; Pu, Liyong; Cheng, Feng

    2015-09-18

    The dysregulation of micro (mi)RNAs is associated with cancer development. The miRNA miR-145 is downregulated in intrahepatic cholangiocarcinoma (ICC); however, its precise role in tumor progression has not yet been elucidated. Novel (nua) kinase family (NUAK)1 functions as an oncogene in various cancers and is a putative target of miR-145 regulation. In this study, we investigated the regulation of NUAK1 by miR-145 in ICC. We found that miR-145 level was significantly decreased in ICC tissue and cell lines, which corresponded with an increase in NUAK1 expression. NUAK1 was found to be a direct target of miR-145 regulation. The overexpression of miR-145 in ICC cell lines inhibited proliferation, growth, and invasion by suppressing NUAK1 expression, which was associated with a decrease in Akt signaling and matrix metalloproteinase protein expression. Similar results were observed by inhibiting NUAK1 expression. These results demonstrate that miR-145 can prevent ICC progression by targeting NUAK1 and its downstream effectors, and can therefore be useful for clinical diagnosis and targeted therapy of ICC. - Highlights: • MiR-145 suppresses ICC proliferation and invasion abilities. • We demonstrated that miR-145 directly targets NUAK1 in ICC. • MiR-145 expression in ICC was associated with Akt signaling and MMPs expression.

  1. Neutron scattering study of spin ordering and stripe pinning in superconducting <mi>La>1.93<mi>Sr>0.07<mi>CuO>4

    SciTech Connect (OSTI)

    Jacobsen, H.; Zaliznyak, I. A.; Savici, A. T.; Winn, B. L.; Chang, S.; Hücker, M.; Gu, G. D.; Tranquada, J. M.

    2015-11-20

    The relationships among charge order, spin fluctuations, and superconductivity in underdoped cuprates remain controversial. We use neutron scattering techniques to study these phenomena in <mi>La>1.93<mi>Sr>0.07<mi>CuO>4 a superconductor with a transition temperature of Tc = 20 K. At T<< Tc, we find incommensurate spin fluctuations with a quasielastic energy spectrum and no sign of a gap within the energy range from 0.2 to 15 meV. A weak elastic magnetic component grows below ~ 10 K, consistent with results from local probes. Regarding the atomic lattice, we have discovered unexpectedly strong fluctuations of the CuO6 octahedra about Cu-O bonds, which are associated with inequivalent O sites within the CuO2 planes. Moreover, we observed a weak elastic (3 30) superlattice peak that implies a reduced lattice symmetry. The presence of inequivalent O sites rationalizes various pieces of evidence for charge stripe order in underdoped La2-xSrxCuO4. The coexistence of superconductivity with quasi-static spin-stripe order suggests the presence of intertwined orders; however, the rotation of the stripe orientation away from the Cu-O bonds might be connected with evidence for a finite gap at the nodal points of the superconducting gap function.

  2. Radiosensitizing Effects of Ectopic miR-101 on Non-Small-Cell Lung Cancer Cells Depend on the Endogenous miR-101 Level

    SciTech Connect (OSTI)

    Chen, Susie; Wang Hongyan; Ng, Wooi Loon; Curran, Walter J.; Wang Ya

    2011-12-01

    Purpose: Previously, we showed that ectopic miR-101 could sensitize human tumor cells to radiation by targeting ATM and DNA-PK catalytic subunit (DNA-PKcs) to inhibit DNA repair, as the endogenous miR-101 levels are low in tumors in general. However, the heterogeneity of human cancers may result in an exception. The purpose of this study was to test the hypothesis that a few tumor cell lines with a high level of endogenous miR-101 would prove less response to ectopic miR-101. Methods and Materials: Fourteeen non-small-cell lung cancer (NSCLC) cell lines and one immortalized non-malignant lung epithelial cell line (NL20) were used for comparing endogenous miR-101 levels by real-time reverse transcription-polymerase chain reaction. Based on the different miR-101 levels, four cell lines with different miR-101 levels were chosen for transfection with a green fluorescent protein-lentiviral plasmid encoding miR-101. The target protein levels were measured by using Western blotting. The radiosensitizing effects of ectopic miR-101 on these NSCLC cell lines were determined by a clonogenic assay and xenograft mouse model. Results: The endogenous miR-101 level was similar or lower in 13 NSCLC cell lines but was 11-fold higher in one cell line (H157) than in NL20 cells. Although ectopic miR-101 efficiently decreased the ATM and DNA-PKcs levels and increased the radiosensitization level in H1299, H1975, and A549 cells, it did not change the levels of the miR-101 targets or radiosensitivity in H157 cells. Similar results were observed in xenograft mice. Conclusions: A small number of NSCLC cell lines could have a high level of endogenous miR-101. The ectopic miR-101 was able to radiosensitize most NSCLC cells, except for the NSCLC cell lines that had a much higher endogenous miR-101 level. These results suggest that when we choose one miRNA as a therapeutic tool, the endogenous level of the miRNA in each tumor should be considered.

  3. Use of H2S to Probe the Active Sites in FeNC Catalysts for the Oxygen Reduction Reaction (ORR) in Acidic Media

    SciTech Connect (OSTI)

    Singh, Deepika; Mamtani, Kuldeep; Bruening, Christopher R.; Miller, Jeffrey T.; Ozkan, Umit S.

    2014-10-01

    H2S has been used as a probe molecule both in an “in situ” poisoning experiment and in intermediate-temperature heat-treatment steps during and after the preparation of FeNC catalysts in an attempt to analyze its effect on their ORR activity. The heat treatments were employed either on the ball-milled precursor of FeNC or after the Ar-NH3 high temperature heat treatments. ORR activity of the H2S-treated catalysts was seen to be significantly lower than the sulfur-free catalysts, whether the sulfur exposure was during a half-cell testing, or as an intermediate-temperature exposure to H2S. The incorporation of sulfur species and interaction of Fe with sulfur were confirmed by characterization using XPS, EXAFS, TPO, and TPD. This study provides crucial evidence regarding differences in active sites in FeNC versus nitrogen-containing carbon nanostructured (CNx) catalysts.

  4. DLEU2, frequently deleted in malignancy, functions as a critical host gene of the cell cycle inhibitory microRNAs miR-15a and miR-16-1

    SciTech Connect (OSTI)

    Lerner, Mikael; Harada, Masako; Loven, Jakob; Castro, Juan; Davis, Zadie; Oscier, David; Henriksson, Marie; Sangfelt, Olle; Grander, Dan; Corcoran, Martin M.

    2009-10-15

    The microRNAs miR-15a and miR-16-1 are downregulated in multiple tumor types and are frequently deleted in chronic lymphocytic leukemia (CLL), myeloma and mantle cell lymphoma. Despite their abundance in most cells the transcriptional regulation of miR-15a/16-1 remains unclear. Here we demonstrate that the putative tumor suppressor DLEU2 acts as a host gene of these microRNAs. Mature miR-15a/miR-16-1 are produced in a Drosha-dependent process from DLEU2 and binding of the Myc oncoprotein to two alterative DLEU2 promoters represses both the host gene transcript and levels of mature miR-15a/miR-16-1. In line with a functional role for DLEU2 in the expression of the microRNAs, the miR-15a/miR-16-1 locus is retained in four CLL cases that delete both promoters of this gene and expression analysis indicates that this leads to functional loss of mature miR-15a/16-1. We additionally show that DLEU2 negatively regulates the G1 Cyclins E1 and D1 through miR-15a/miR-16-1 and provide evidence that these oncoproteins are subject to miR-15a/miR-16-1-mediated repression under normal conditions. We also demonstrate that DLEU2 overexpression blocks cellular proliferation and inhibits the colony-forming ability of tumor cell lines in a miR-15a/miR-16-1-dependent way. Together the data illuminate how inactivation of DLEU2 promotes cell proliferation and tumor progression through functional loss of miR-15a/miR-16-1.

  5. Genome-Wide Analysis of miRNA targets in Brachypodium and Biomass Energy Crops

    SciTech Connect (OSTI)

    Green, Pamela J.

    2015-08-11

    MicroRNAs (miRNAs) contribute to the control of numerous biological processes through the regulation of specific target mRNAs. Although the identities of these targets are essential to elucidate miRNA function, the targets are much more difficult to identify than the small RNAs themselves. Before this work, we pioneered the genome-wide identification of the targets of Arabidopsis miRNAs using an approach called PARE (German et al., Nature Biotech. 2008; Nature Protocols, 2009). Under this project, we applied PARE to Brachypodium distachyon (Brachypodium), a model plant in the Poaceae family, which includes the major food grain and bioenergy crops. Through in-depth global analysis and examination of specific examples, this research greatly expanded our knowledge of miRNAs and target RNAs of Brachypodium. New regulation in response to environmental stress or tissue type was found, and many new miRNAs were discovered. More than 260 targets of new and known miRNAs with PARE sequences at the precise sites of miRNA-guided cleavage were identified and characterized. Combining PARE data with the small RNA data also identified the miRNAs responsible for initiating approximately 500 phased loci, including one of the novel miRNAs. PARE analysis also revealed that differentially expressed miRNAs in the same family guide specific target RNA cleavage in a correspondingly tissue-preferential manner. The project included generation of small RNA and PARE resources for bioenergy crops, to facilitate ongoing discovery of conserved miRNA-target RNA regulation. By associating specific miRNA-target RNA pairs with known physiological functions, the research provides insights about gene regulation in different tissues and in response to environmental stress. This, and release of new PARE and small RNA data sets should contribute basic knowledge to enhance breeding and may suggest new strategies for improvement of biomass energy crops.

  6. miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A

    SciTech Connect (OSTI)

    Shao, Mingchen; Geng, Yiwei; Lu, Peng; Xi, Ying; Wei, Sidong; Wang, Liuxing; Fan, Qingxia; Ma, Wang

    2015-09-04

    MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention. - Highlights: • miR-4295 mimics promote the proliferation and invasion of ATC cells. • miR-4295 inhibitors inhibit the proliferation and invasion of ATC cells. • miR-4295 targets 3′UTR of CDKN1A in ATC cells. • miR-4295 negatively regulates CDKN1A in ATC cells.

  7. Microfluidic Molecular Assay Platform for the Detection of miRNAs...

    Office of Scientific and Technical Information (OSTI)

    Article: Microfluidic Molecular Assay Platform for the Detection of miRNAs, mRNAs, Proteins, and Post-translational Modifications at Single-cell Resolution. Citation Details...

  8. Resonant tunneling with high peak to valley current ratio in SiO{sub 2}/nc-Si/SiO{sub 2} multi-layers at room temperature

    SciTech Connect (OSTI)

    Chen, D. Y.; Sun, Y.; He, Y. J.; Xu, L.; Xu, J.

    2014-01-28

    We have investigated carrier transport in SiO{sub 2}/nc-Si/SiO{sub 2} multi-layers by room temperature current-voltage measurements. Resonant tunneling signatures accompanied by current peaks are observed. Carrier transport in the multi-layers were analyzed by plots of ln(I/V{sup 2}) as a function of 1/V and ln(I) as a function of V{sup 1/2}. Results suggest that besides films quality, nc-Si and barrier sub-layer thicknesses are important parameters that restrict carrier transport. When thicknesses are both small, direct tunneling dominates carrier transport, resonant tunneling occurs only at certain voltages and multi-resonant tunneling related current peaks can be observed but with peak to valley current ratio (PVCR) values smaller than 1.5. When barrier thickness is increased, trap-related and even high field related tunneling is excited, causing that multi-current peaks cannot be observed clearly, only one current peak with higher PVCR value of 7.7 can be observed. While if the thickness of nc-Si is large enough, quantum confinement is not so strong, a broad current peak with PVCR value as high as 60 can be measured, which may be due to small energy difference between the splitting energy levels in the quantum dots of nc-Si. Size distribution in a wide range may cause un-controllability of the peak voltages.

  9. Groundwater protection for the NuMI project

    SciTech Connect (OSTI)

    Wehmann, A.; Smart, W.; Menary, S.; Hylen, J.; Childress, S.

    1997-10-01

    The physics requirements for the long base line neutrino oscillation experiment MINOS dictate that the NuMI beamline be located in the aquifer at Fermilab. A methodology is described for calculating the level of radioactivation of groundwater caused by operation of this beamline. A conceptual shielding design for the 750 meter long decay pipe is investigated which would reduce radioactivation of the groundwater to below government standards. More economical shielding designs to meet these requirements are being explored. Also, information on local geology, hydrogeology, government standards, and a glossary have been included.

  10. miR-129 suppresses tumor cell growth and invasion by targeting PAK5 in hepatocellular carcinoma

    SciTech Connect (OSTI)

    Zhai, Jian; Qu, Shuping; Li, Xiaowei; Zhong, Jiaming; Chen, Xiaoxia; Qu, Zengqiang; Wu, Dong

    2015-08-14

    Emerging evidence suggests that microRNAs (miRNAs) play important roles in regulating HCC development and progression; however, the mechanisms by which their specific functions and mechanisms remained to be further explored. miR-129 has been reported in gastric cancers, lung cancer and colon cancer. In this study, we disclosed a new tumor suppresser function of miR-129 in HCC. We also found the downregulation of miR-129 occurred in nearly 3/4 of the tumors examined (56/76) compared with adjacent nontumorous tissues, which was more importantly, correlated to the advanced stage and vascular invasion. We then demonstrated that miR-129 overexpression attenuated HCC cells proliferation and invasion, inducing apoptosis in vitro. Moreover, we used miR-129 antagonist and found that anti-miR-129 promoted HCC cells malignant phenotypes. Mechanistically, our further investigations revealed that miR-129 suppressed cell proliferation and invasion by targeting the 3’-untranslated region of PAK5, as well as miR-129 silencing up-regulated PAK5 expression. Moreover, miR-129 expression was inversely correlated with PAK5 expression in 76 cases of HCC samples. RNA interference of PAK5 attenuated anti-miR-129 mediated cell proliferation and invasion in HCC cells. Taken together, these results demonstrated that miR-129 suppressed tumorigenesis and progression by directly targeting PAK5, defining miR-129 as a potential treatment target for HCC. - Highlights: • Decreased of miR-129 is found in HCC and associated with advanced stage and metastasis. • miR-129 suppresses proliferation and invasion of HCC cells. • miR-129 directly targets the 3′ UTR of PAK5 and diminishes PAK5 expression. • PAK5 is involved in miR-129 mediated suppression functions.

  11. MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19

    SciTech Connect (OSTI)

    Shan, Nianxi; Shen, Liangfang; Wang, Jun; He, Dan; Duan, Chaojun

    2015-01-02

    Highlights: • Decreased miR-153 and up-regulated ADAM19 are correlated with NSCLC pathology. • MiR-153 inhibits the proliferation and migration and invasion of NSCLC cells in vitro. • ADAM19 is a direct target of miR-153. • ADAM19 is involved in miR-153-suppressed migration and invasion of NSCLC cells. - Abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3′UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which was similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.

  12. Transcriptional regulation of miR-146b by C/EBPβ LAP2 in esophageal cancer cells

    SciTech Connect (OSTI)

    Li, Junxia; Shan, Fabo; Xiong, Gang; Wang, Ju-Ming; Wang, Wen-Lin; Xu, Xueqing; Bai, Yun

    2014-03-28

    Highlights: • MiR-146b promotes esophageal cancer cell proliferation. • MiR-146b inhibits esophageal cancer cell apoptosis. • C/EBPβ directly binds to miR-146b promoter conserved region. • MiR-146b is up-regulated by C/EBPβ LAP2 transcriptional activation. - Abstract: Recent clinical study indicated that up-regulation of miR-146b was associated with poor overall survival of patients in esophageal squamous cell carcinoma. However, the underlying mechanism of miR-146b dysregulation remains to be explored. Here we report that miR-146b promotes cell proliferation and inhibits cell apoptosis in esophageal cancer cell lines. Mechanismly, two C/EBPβ binding motifs are located in the miR-146b promoter conserved region. Among the three isoforms of C/EBPβ, C/EBPβ LAP2 positively regulated miR-146b expression and increases miR-146b levels in a dose-dependent manner through transcription activation of miR-146b gene. Together, these results suggest a miR-146b regulatory mechanism involving C/EBPβ, which may contribute to the up-regulation of miR-146b in esophageal squamous cell carcinoma.

  13. miR-30a suppresses breast cancer cell proliferation and migration by targeting Eya2

    SciTech Connect (OSTI)

    Fu, Jing; Xu, Xiaojie; Kang, Lei; Zhou, Liying; Wang, Shibin; Lu, Juming; Cheng, Long; Fan, Zhongyi; Yuan, Bin; Tian, Peirong; Zheng, Xiaofei; Yu, Chengze; Ye, Qinong; Lv, Zhaohui

    2014-03-07

    Highlights: • miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. • The miR-30a/EYA2 axis regulates breast cancer cell proliferation and migration. • The miR-30a/EYA2 axis modulates G1/S cell cycle progression. • The miR-30a/EYA2 axis is dysregulated in breast cancer patients. - Abstract: Eye absent (Eya) proteins are involved in cell fate determination in a broad spectrum of cells and tissues. Aberrant expression of Eya2 has been documented in a variety of cancers and correlates with clinical outcome. However, whether microRNAs (miRNAs) can regulate Eya2 expression remains unknown. Here, we show that miR-30a represses Eya2 expression by binding to the 3′-untranslated region of Eya2. Overexpression of Eya2 in miR-30a-transfected breast cancer cells effectively rescued the inhibition of cell proliferation and migration caused by miR-30a. Knockdown of Eya2 by small-interfering RNA (siRNA) in breast cancer cells mimicked the effect induced by miR-30a and abolished the ability of miR-30a to regulate breast cancer cell proliferation and migration. The miR-30a/Eya2 axis could regulate G1/S cell cycle progression, accompanied by the modulation of expression of cell cycle-related proteins, including cyclin A, cyclin D1, cyclin E, and c-Myc. Moreover, miR-30a expression was downregulated in breast cancer patients, and negatively correlated with Eya2, which was upregulated in breast cancer patients. These data suggest that the miR-30a/Eya2 axis may play an important role in breast cancer development and progression and that miR-30a activation or Eya2 inhibition may be a useful strategy for cancer treatment.

  14. miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7

    SciTech Connect (OSTI)

    Zhou, Chuanyi; Shen, Liangfang; Mao, Lei; Wang, Bing; Li, Yang; Yu, Huizhi

    2015-02-27

    MicroRNAs (miRNAs) are involved in the cervical carcinogenesis and progression. In this study, we investigated the role of miR-92a in progression and invasion of cervical cancer. MiR-92a was significantly upregulated in cervical cancer tissues and cell lines. Overexpression of miR-92a led to remarkably enhanced proliferation by promoting cell cycle transition from G1 to S phase and significantly enhanced invasion of cervical cancer cells, while its knockdown significantly reversed these cellular events. Bioinformatics analysis suggested F-box and WD repeat domain-containing 7 (FBXW7) as a novel target of miR-92a, and miR-92a suppressed the expression level of FBXW7 mRNA by direct binding to its 3′-untranslated region (3′UTR). Expression of miR-92a was negatively correlated with FBXW7 in cervical cancer tissues. Furthermore, Silencing of FBXW7 counteracted the effects of miR-92a suppression, while its overexpression reversed oncogenic effects of miR-92a. Together, these findings indicate that miR-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, suggesting miR-92a as a potential novel diagnostic and therapeutic target of cervical cancer. - Highlights: • miR-92a is elevated in cervical cancer tissues and cell lines. • miR-92a promotes cervical cancer cell proliferation, cell cycle transition from G1 to S phase and invasion. • FBXW7 is a direct target of miR-92a. • FBXW7 counteracts the oncogenic effects of miR-92a on cervical cancer cells.

  15. miR-182 targets CHL1 and controls tumor growth and invasion in papillary thyroid carcinoma

    SciTech Connect (OSTI)

    Zhu, Hongling; Fang, Jin; Zhang, Jichen; Zhao, Zefei; Liu, Lianyong; Wang, Jingnan; Xi, Qian; Gu, Mingjun

    2014-07-18

    Highlights: miR-182 and CHL1 expression patterns are negatively correlated. CHL1 is a direct target of miR-182 in PTC cells. miR-182 suppression inhibits PTC cell growth and invasion. CHL1 is involved in miR-182-mediated cell behavior. - Abstract: In this study, we investigated the role and underlying mechanism of action of miR-182 in papillary thyroid carcinoma (PTC). Bioinformatics analysis revealed close homolog of LI (CHL1) as a potential target of miR-182. Upregulation of miR-182 was significantly correlated with CHL1 downregulation in human PTC tissues and cell lines. miR-182 suppressed the expression of CHL1 mRNA through direct targeting of the 3?-untranslated region (3?-UTR). Downregulation of miR-182 suppressed growth and invasion of PTC cells. Silencing of CHL1 counteracted the effects of miR-182 suppression, while its overexpression mimicked these effects. Our data collectively indicate that miR-182 in PTC promotes cell proliferation and invasion through direct suppression of CHL1, supporting the potential utility of miR-182 inhibition as a novel therapeutic strategy against PTC.

  16. Non-canonical microRNAs miR-320 and miR-702 promote proliferation in Dgcr8-deficient embryonic stem cells

    SciTech Connect (OSTI)

    Kim, Byeong-Moo; Choi, Michael Y.

    2012-09-21

    Highlights: Black-Right-Pointing-Pointer Embryonic stem cells (ESCs) lacking non-canonical miRNAs proliferate slower. Black-Right-Pointing-Pointer miR-320 and miR-702 are two non-canonical miRNAs expressed in ESCs. Black-Right-Pointing-Pointer miR-320 and miR-702 promote proliferation of Dgcr8-deficient ESCs. Black-Right-Pointing-Pointer miR-320 targets p57 and helps to release Dgcr8-deficient ESCs from G1 arrest. Black-Right-Pointing-Pointer miR-702 targets p21 and helps to release Dgcr8-deficient ESCs from G1 arrest. -- Abstract: MicroRNAs are known to contribute significantly to stem cell phenotype by post-transcriptionally regulating gene expression. Most of our knowledge of microRNAs comes from the study of canonical microRNAs that require two sequential cleavages by the Drosha/Dgcr8 heterodimer and Dicer to generate mature products. In contrast, non-canonical microRNAs bypass the cleavage by the Drosha/Dgcr8 heterodimer within the nucleus but still require cytoplasmic cleavage by Dicer. The function of non-canonical microRNAs in embryonic stem cells (ESCs) remains obscure. It has been hypothesized that non-canonical microRNAs have important roles in ESCs based upon the phenotypes of ESC lines that lack these specific classes of microRNAs; Dicer-deficient ESCs lacking both canonical and non-canonical microRNAs have much more severe proliferation defect than Dgcr8-deficient ESCs lacking only canonical microRNAs. Using these cell lines, we identified two non-canonical microRNAs, miR-320 and miR-702, that promote proliferation of Dgcr8-deficient ESCs by releasing them from G1 arrest. This is accomplished by targeting the 3 Prime -untranslated regions of the cell cycle inhibitors p57 and p21 and thereby inhibiting their expression. This is the first report of the crucial role of non-canonical microRNAs in ESCs.

  17. NuMI proton kicker extraction magnet termination resistor system

    SciTech Connect (OSTI)

    Reeves, S.R.; Jensen, C.C.; /Fermilab

    2005-05-01

    The temperature stability of the kicker magnet termination resistor assembly directly affects the field flatness and amplitude stability. Comprehensive thermal enhancements were made to the existing Main Injector resistor assembly design to satisfy NuMI performance specifications. Additionally, a fluid-processing system utilizing Fluorinert{reg_sign} FC-77 high-voltage dielectric was built to precisely control the setpoint temperature of the resistor assembly from 70 to 120F, required to maintain constant resistance during changing operational modes. The Fluorinert{reg_sign} must be continually processed to remove hazardous breakdown products caused by radiation exposure to prevent chemical attack of system components. Design details of the termination resistor assembly and Fluorinert{reg_sign} processing system are described. Early performance results will be presented.

  18. Downregulation of miRNA-30c and miR-203a is associated with hepatitis C virus core protein-induced epithelial–mesenchymal transition in normal hepatocytes and hepatocellular carcinoma cells

    SciTech Connect (OSTI)

    Liu, Dongjing; Wu, Jilin; Liu, Meizhou; Yin, Hui; He, Jiantai; Zhang, Bo

    2015-09-04

    Hepatitis C virus (HCV) Core protein has been demonstrated to induce epithelial–mesenchymal transition (EMT) and is associated with cancer progression of hepatocellular carcinoma (HCC). However, how the Core protein regulates EMT is still unclear. In this study, HCV Core protein was overexpressed by an adenovirus. The protein levels of EMT markers were measured by Western blot. The xenograft animal model was established by inoculation of HepG2 cells. Results showed that ectopic expression of HCV core protein induced EMT in L02 hepatocytes and HepG2 tumor cells by upregulating vimentin, Sanl1, and Snal2 expression and downregulating E-cadherin expression. Moreover, Core protein downregulated miR-30c and miR-203a levels in L02 and HepG2 cells, but artificial expression of miR-30c and miR-203a reversed Core protein-induced EMT. Further analysis showed that ectopic expression of HCV core protein stimulated cell proliferation, inhibited apoptosis, and increased cell migration, whereas artificial expression of miR-30c and miR-203a significantly reversed the role of Core protein in these cell functions in L02 and HepG2 cells. In the HepG2 xenograft tumor models, artificial expression of miR-30c and miR-203a inhibited EMT and tumor growth. Moreover, L02 cells overexpressing Core protein can form tumors in nude mice. In HCC patients, HCV infection significantly shortened patients' survival time, and loss of miR-30c and miR-203 expression correlated with poor survival. In conclusion, HCV core protein downregulates miR-30c and miR-203a expression, which results in activation of EMT in normal hepatocytes and HCC tumor cells. The Core protein-activated-EMT is involved in the carcinogenesis and progression of HCC. Loss of miR-30c and miR-203a expression is a marker for the poor prognosis of HCC. - Highlights: • HCV core protein downregulates miR-30c and miR-203a expression. • Downregulation of miR-30c and miR-203a activates EMT. • Activated-EMT is involved in the

  19. miR-128 and its target genes in tumorigenesis and metastasis

    SciTech Connect (OSTI)

    Li, Molin, E-mail: molin_li@hotmail.com [Dalian Medical University, Dalian 116044 (China); Fu, Weiming [Center for Food Safety and Environmental Technology, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, Guangzhou 511458 (China); Wo, Lulu; Shu, Xiaohong [Dalian Medical University, Dalian 116044 (China); Liu, Fang [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China); Li, Chuangang, E-mail: li_chuangang@sina.com [The second affiliated hospital of Dalian Medical University, Dalian 116023 (China)

    2013-12-10

    MicroRNAs (miRNAs) are a class of endogenous, non-coding, 1824 nucleotide length single-strand RNAs that could modulate gene expression at post-transcriptional level. Previous studies have shown that miR-128 enriched in the brain plays an important role in the development of nervous system and the maintenance of normal physical functions. Aberrant expression of miR-128 has been detected in many types of human tumors and its validated target genes are involved in cancer-related biological processes such as cell proliferation, differentiation and apoptosis. In this review, we will summarize the roles of miR-128 and its target genes in tumorigenesis and metastasis. - Highlights: Aberrant expression of miR-128 can be observed in many kinds of malignant tumors. The molecular mechanisms regulating miR-128 expression are elucidated. Roles of miR-128 and its target genes in tumorigenesis and metastasis are summarized.

  20. miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation

    SciTech Connect (OSTI)

    Mizuno, Yosuke; Yagi, Ken; Tokuzawa, Yoshimi; Kanesaki-Yatsuka, Yukiko; Suda, Tatsuo; Katagiri, Takenobu; Fukuda, Toru; Maruyama, Masayoshi; Okuda, Akihiko; Amemiya, Tomoyuki; Kondoh, Yasumitsu; Tashiro, Hideo; Okazaki, Yasushi

    2008-04-04

    Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.

  1. Natural Gas Heat Pump and Air Conditioner

    Office of Energy Efficiency and Renewable Energy (EERE)

    Lead Performer: Thermolift - Stony Brook, NY Partners: -- New York State Energy Research & Development Authority - Albany, NY -- Stony Brook University - Stony Brook, NY -- Oak Ridge National Laboratory - Oak Ridge, TN -- National Grid - Washington, DC -- Applied Thermodynamic Apparatus (ATA) - Ann Arbor, MI -- Fala Technologies - Kingston, NY -- LoDolce - Saugerties, NY

  2. CT NC0

    Office of Legacy Management (LM)

    P233scNs VISITED . R..Hammes- General Sales Manager, W, C. Smith - Tolhurst Centrifugals Division L, Jauhola - Ch:cef QpTication Engineer, Sales Maml;er, Tolhurst Centrifugals ...

  3. Overexpression of miR-206 suppresses glycolysis, proliferation and migration in breast cancer cells via PFKFB3 targeting

    SciTech Connect (OSTI)

    Ge, Xin; Lyu, Pengwei; Cao, Zhang; Li, Jingruo; Guo, Guangcheng; Xia, Wanjun; Gu, Yuanting

    2015-08-07

    miRNAs, sorting as non-coding RNAs, are differentially expressed in breast tumor and act as tumor promoters or suppressors. miR-206 could suppress the progression of breast cancer, the mechanism of which remains unclear. The study here was aimed to investigate the effect of miR-206 on human breast cancers. We found that miR-206 was down-regulated while one of its predicted targets, 6-Phosphofructo-2-kinase (PFKFB3) was up-regulated in human breast carcinomas. 17β-estradiol dose-dependently decreased miR-206 expression as well as enhanced PFKFB3 mRNA and protein expression in estrogen receptor α (ERα) positive breast cancer cells. Furthermore, we identified that miR-206 directly interacted with 3′-untranslated region (UTR) of PFKFB3 mRNA. miR-206 modulated PFKFB3 expression in MCF-7, T47D and SUM159 cells, which was influenced by 17β-estradiol depending on ERα expression. In addition, miR-206 overexpression impeded fructose-2,6-bisphosphate (F2,6BP) production, diminished lactate generation and reduced cell proliferation and migration in breast cancer cells. In conclusion, our study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration. - Highlights: • miR-206 was down-regulated and PFKFB3 was up-regulated in human breast carcinomas. • 17β-estradiol regulated miR-206 and PFKFB3 expression in ERα+ cancer cells. • miR-206directly interacted with 3′-UTR of PFKFB3 mRNA. • miR-206 fructose-2,6-bisphosphate (F2,6BP) impeded production and lactate generation. • miR-206 reduced cell proliferation and migration in breast cancer cells.

  4. AMENDMENT OF SOLICITATION/MODIFICATlON OF CONTRACT MI54 I See...

    National Nuclear Security Administration (NNSA)

    MI54 I See Block 16C I REQ. NO. Babcock & Wilcox Technical Services Pantex, LLC PO Box 30020 Amarillo, TX 79120 2. AMENDMENTIMODIFICATION NO. 1 3. EFFECTIVE DATE 1 4. ...

  5. File:USDA-CE-Production-GIFmaps-MI.pdf | Open Energy Information

    Open Energy Info (EERE)

    MI.pdf Jump to: navigation, search File File history File usage Michigan Ethanol Plant Locations Size of this preview: 463 599 pixels. Other resolution: 464 600 pixels. Full...

  6. Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI |

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Department of Energy Sault Ste. Marie Tribe of Chippewa Indians, MI Climate Action Champions: Sault Ste. Marie Tribe of Chippewa Indians, MI The Sault Ste. Marie Tribe of Chippewa Indians is a 44,000-strong federally recognized Indian tribe that is an economic, social and cultural force in its community across the eastern Upper Peninsula counties of Chippewa, Luce, Mackinac, Schoolcraft, Alger, Delta and Marquette, with housing and tribal centers, casinos, and other enterprises that employ

  7. padd map

    U.S. Energy Information Administration (EIA) Indexed Site

    FL PADD 4: Rocky Mountain PADD 5: West Coast PADD 2: Midwest PADD 1: East Coast PADD 3: Gulf Coast PADD1A: New England PADD1B: Central Atlantic PADD1C: Lower Atlantic Petroleum Administration for Defense Districts AK HI WA OR CA NV AZ MT WY CO UT ID ND SD NE KS OK MO MN WI MI IL IN OH KY TN IA NM TX AR LA AL MS WV VA NC SC GA FL ME NH VT NY PA NJ MD DE MA CT RI

  8. printer_friendly_org_chart

    Gasoline and Diesel Fuel Update (EIA)

    FL PADD 4: Rocky Mountain PADD 5: West Coast PADD 2: Midwest PADD 1: East Coast PADD 3: Gulf Coast PADD1A: New England PADD1B: Central Atlantic PADD1C: Lower Atlantic Petroleum Administration for Defense Districts AK HI WA OR CA NV AZ MT WY CO UT ID ND SD NE KS OK MO MN WI MI IL IN OH KY TN IA NM TX AR LA AL MS WV VA NC SC GA FL ME NH VT NY PA NJ MD DE MA CT RI

    Administrator U.S. Energy Information Administration Adam Sieminski Deputy Administrator Howard Gruenspecht Assistant Administrator

  9. paper-LSPP16

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FL PADD 4: Rocky Mountain PADD 5: West Coast PADD 2: Midwest PADD 1: East Coast PADD 3: Gulf Coast PADD1A: New England PADD1B: Central Atlantic PADD1C: Lower Atlantic Petroleum Administration for Defense Districts AK HI WA OR CA NV AZ MT WY CO UT ID ND SD NE KS OK MO MN WI MI IL IN OH KY TN IA NM TX AR LA AL MS WV VA NC SC GA FL ME NH VT NY PA NJ MD DE MA CT RI

    pantex On Womens Equality Day, we celebrate NNSA's talented Women in STEM NNSA's systems administrators keep the computers running

  10. Department of Energy (DOE) OpenNet documents

    Office of Scientific and Technical Information (OSTI)

    Account Request *First Name: *Last Name: *Organization: *Phone: *Email: Fax: *Address: *City: *State: Select AL AK AZ AR CA CO CT DE DC FL GA HI ID IL IN IA KS KY LA ME MD MA MI MN MS MO MT NE NV NH NJ NM NY NC ND OH OK OR PA RI SC SD TN TX UT VT VA WA WV WI WY Zip Code: *Affiliation: Select Federal Contractor *Access Level: Select View Release Official Releasing Official: release *Site Input Code: Select A2EDAP - Atmosphere to Electrons (A2e) Data Archive and Portal, Pacific Northwest National

  11. DOE Hydrogen and Fuel Cell Overview

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    DOE Hydrogen & Fuel Cell Overview Dr. Sunita Satyapal Program Manager U.S. Department of Energy Fuel Cell Technologies Program DOE/CESA/TTC Hydrogen and Fuel Cells Webinar December 14, 2010 2 Examples of DOE-funded Partners and Locations - Fuel Cell Technologies Program TX NM AZ NC AR CA CO HI WA IL KY MA MN MO MS AL NV TN UT WV ID FL MI ND OR OH IN MT WY IO NE KS OK AK LA GA WI SC VA PA DE MD DC NJ NY RI CT VT NH ME SD Source: US DOE 12/2010 2 3 Fuel Cells: Addressing Energy Challenges 4

  12. Print

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    < 5k 0 < 50k < 100k < 250k < 500k < 1M > 1M > 5M > 10M DE MD DC MA RI NJ AZ UT WY ID OR WA CA TX OK KS CO NE SD ND MN WI IL IA MO AR LA MS AL FL GA TN KY IN OH MI ME NH CT VT NY PA WV VA NC SC MT AK HI NV NM Princeton Plasma Physics Laboratory Procured Materials and Services 2015 (> $35M) Small business procurements in US: $14.73M

  13. Buildings Energy Data Book: 3.9 Educational Facilities

    Buildings Energy Data Book [EERE]

    6 2010 Regional New Construction and Renovations Expenditures for Public K-12 Schools ($Million) Region New Schools Additions Renovation Total Region 1 (CT, MA, ME, NH, RI, VT) Region 2 (NJ, NY, PA) Region 3 (DE, MD, VA, WV) Region 4 (KY, NC, SC, TN) Region 5 (AL, FL, GA, MS) Region 6 (IN, MI, OH) Region 7 (IL, MN, WI) Region 8 (IA, KS, MO, NE) Region 9 (AR, LA, OK, TX) Region 10 (CO, MT, ND, NM, SD, UT, WY) Region 11 (AZ, CA, HI, NV) Region 12 (AK, ID, OR, WA) Total Source(s): School Planning

  14. Training Session: Buffalo, NY

    Broader source: Energy.gov [DOE]

    This 3.5-hour training provides builders with a comprehensive review of zero energy-ready home construction including the business case, detailed specifications, and opportunities to be recognized...

  15. NY Green Bank

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Bank Panel 1: Attracting and Maintaining Capital for Energy Transmission, Storage, and ... clean energy capital markets for large scale infrastructure are deep and robust. ...

  16. NY-%-3 P

    Office of Legacy Management (LM)

    ... Utilized Manhattan Engineer DistrictAtomic Energy Commission Sites Program, " DOE... in Western New York 0 One Typical Chest X-Ray 0 Maximum Dose Caused by 0 Exposure to ...

  17. U.S. Natural Gas Imports by Pipeline from Mexico

    Gasoline and Diesel Fuel Update (EIA)

    Argentina Sabine Pass, LA Total To Barbados Miami, FL Total To Brazil Freeport, TX Sabine Pass, LA Total to Canada Eastport, ID Calais, ME Detroit, MI Marysville, MI Port Huron, MI Crosby, ND Portal, ND Sault St. Marie, MI St. Clair, MI Noyes, MN Babb, MT Havre, MT Port of Morgan, MT Sherwood, ND Pittsburg, NH Buffalo, NY Grand Island, NY Massena, NY Niagara Falls, NY Waddington, NY Sumas, WA Sweetgrass, MT Total to Chile Sabine Pass, LA Total to China Kenai, AK Sabine Pass, LA Total to Egypt

  18. U.S. Natural Gas Imports by Pipeline from Canada

    Annual Energy Outlook [U.S. Energy Information Administration (EIA)]

    Barbados Total To Brazil Freeport, TX Sabine Pass, LA Total to Canada Eastport, ID Calais, ME Detroit, MI Marysville, MI Port Huron, MI Crosby, ND Portal, ND Sault St. Marie, MI St. Clair, MI Noyes, MN Warroad, MN Babb, MT Havre, MT Port of Morgan, MT Sherwood, ND Pittsburg, NH Buffalo, NY Grand Island, NY Massena, NY Niagara Falls, NY Waddington, NY Sumas, WA Sweetgrass, MT Total to Chile Sabine Pass, LA Total to China Kenai, AK Sabine Pass, LA Total to Egypt Freeport, TX Total to India

  19. U.S. Total Exports

    U.S. Energy Information Administration (EIA) Indexed Site

    Barbados Total To Brazil Freeport, TX Sabine Pass, LA Total to Canada Eastport, ID Calais, ME Detroit, MI Marysville, MI Port Huron, MI Crosby, ND Portal, ND Sault St. Marie, MI St. Clair, MI Noyes, MN Warroad, MN Babb, MT Havre, MT Port of Morgan, MT Sherwood, ND Pittsburg, NH Buffalo, NY Grand Island, NY Massena, NY Niagara Falls, NY Waddington, NY Sumas, WA Sweetgrass, MT Total to Chile Sabine Pass, LA Total to China Kenai, AK Sabine Pass, LA Total to Egypt Freeport, TX Total to India

  20. miR-196a targets netrin 4 and regulates cell proliferation and migration of cervical cancer cells

    SciTech Connect (OSTI)

    Zhang, Jie; Zheng, Fangxia; Yu, Gang; Yin, Yanhua; Lu, Qingyang

    2013-11-01

    Highlights: miR-196a was overexpressed in cervical cancer tissue compared to normal tissue. miR-196a expression elevated proliferation and migration of cervical cancer cells. miR-196a inhibited NTN4 expression by binding 3?-UTR region of NTN4 mRNA. NTN4 inversely correlated with miR-196a expression in cervical tissue and cell line. NTN4 expression was low in cervical cancer tissue compared to normal tissue. -- Abstract: Recent research has uncovered tumor-suppressive and oncogenic potential of miR-196a in various tumors. However, the expression and mechanism of its function in cervical cancer remains unclear. In this study, we assess relative expression of miR-196a in cervical premalignant lesions, cervical cancer tissues, and four cancer cell lines using quantitative real-time PCR. CaSki and HeLa cells were treated with miR-196a inhibitors, mimics, or pCDNA/miR-196a to investigate the role of miR-196a in cancer cell proliferation and migration. We demonstrated that miR-196a was overexpressed in cervical intraepithelial neoplasia 23 and cervical cancer tissue. Moreover, its expression contributes to the proliferation and migration of cervical cancer cells, whereas inhibiting its expression led to a reduction in proliferation and migration. Five candidate targets of miR-196a chosen by computational prediction and Cervical Cancer Gene Database search were measured for their mRNA in both miR-196a-overexpressing and -depleted cancer cells. Only netrin 4 (NTN4) expression displayed an inverse association with miR-196a. Fluorescent reporter assays revealed that miR-196a inhibited NTN4 expression by targeting one binding site in the 3?-untranslated region (3?-UTR) of NTN4 mRNA. Furthermore, qPCR and Western blot assays verified NTN4 expression was downregulated in cervical cancer tissues compared to normal controls, and in vivo mRNA level of NTN4 inversely correlated with miR-196a expression. In summary, our findings provide new insights about the functional role of

  1. miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1

    SciTech Connect (OSTI)

    Xu, Zhengyu; Wang, Tao

    2014-06-27

    Highlights: • miR-214 is upregulated in human OS tissues and inversely correlated with LZTS1 expression. • miR-214 directly targets LZTS1 by binding to its 3′-UTR. • miR-214 promotes OS cell proliferation, invasion and tumor growth. • Overexpression of LZTS1 reverses miR-214-induced proliferation and invasion of OS cells. - Abstract: Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealed leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3′-untranslated region (3′-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.

  2. miR-421 induces cell proliferation and apoptosis resistance in human nasopharyngeal carcinoma via downregulation of FOXO4

    SciTech Connect (OSTI)

    Chen, Liang; Department of Otolaryngology, Guangzhou General Hospital of PLA Guangzhou Command, Guangzhou 510010 ; Tang, Yanping; Wang, Jian; Yan, Zhongjie; Xu, Ruxiang

    2013-06-14

    Highlights: •miR-421 is upregulated in nasopharyngeal carcinoma. •miR-421 induces cell proliferation and apoptosis resistance. •FOXO4 is a direct and functional target of miR-421. -- Abstract: microRNAs have been demonstrated to play important roles in cancer development and progression. Hence, identifying functional microRNAs and better understanding of the underlying molecular mechanisms would provide new clues for the development of targeted cancer therapies. Herein, we reported that a microRNA, miR-421 played an oncogenic role in nasopharyngeal carcinoma. Upregulation of miR-421 induced, whereas inhibition of miR-421 repressed cell proliferation and apoptosis resistance. Furthermore, we found that upregulation of miR-421 inhibited forkhead box protein O4 (FOXO4) signaling pathway following downregulation of p21, p27, Bim and FASL expression by directly targeting FOXO4 3′UTR. Additionally, we demonstrated that FOXO4 expression is critical for miR-421-induced cell growth and apoptosis resistance. Taken together, our findings not only suggest that miR-421 promotes nasopharyngeal carcinoma cell proliferation and anti-apoptosis, but also uncover a novel regulatory mechanism for inactivation of FOXO4 in nasopharyngeal carcinoma.

  3. Energy Technology Division Energy Technology Division Energy...

    Office of Scientific and Technical Information (OSTI)

    ... Motors Corp., Lockport, NY S. Smialowska, Ohio State University, Columbus R. E. Smith, Altran Corp., Huntersville, NC U.S. Department of Energy, Office of Energy Research, ...

  4. Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

    SciTech Connect (OSTI)

    Zhou, Yuan; Wang, Hui; Wang, Cong; Qiu, Xuefeng; Benson, Mikael; Yin, Xiaoqin; Xiang, Zou; Li, Dongmei; and others

    2015-08-15

    Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system. - Highlights: • miRNAs were altered in Sertoli cells exposed to MC-LR. • Alerted genes were involved in different cell functions including the cell morphology. • MC-LR adversely affected Sertoli cell junction formation through the regulating miRNAs.

  5. Source characterization and control technology assessment of methylene chloride emissions from Eastman Kodak Company, Rochester, NY. Final report, July 1988-April 1989

    SciTech Connect (OSTI)

    Walata, S.A.; Rehm, R.M.

    1989-07-01

    This report gives results of an assessment of potential control technologies for methylene chloride (also known as dichloromethane or DCM) emission sources at Eastman Kodak Company's Kodak Park facility in Rochester, NY. DCM is a solvent used by Kodak in the manufacture of cellulose triacetate film support. Work has involved: a plant visit where major DCM emission sources were inspected, and evaluation of current and potential control technologies for the DCM emission sources. The report contains information gathered during the plant visit to the Kodak Park facility. Included are emission estimates determined by Kodak of all emission points greater than 8000 lb (3600 kg)/yr DCM, as well as a description of each point observed during the visit. Also included are results of an evaluation of control technologies that might be applied to the major emission sources. A cost analysis of different add-on control devices is provided for four of the uncontrolled emission points.

  6. Ionizing Radiation–Inducible miR-27b Suppresses Leukemia Proliferation via Targeting Cyclin A2

    SciTech Connect (OSTI)

    Wang, Bo; Li, Dongping; Kovalchuk, Anna; Litvinov, Dmitry; Kovalchuk, Olga

    2014-09-01

    Purpose: Ionizing radiation is a common carcinogen that is important for the development of leukemia. However, the underlying epigenetic mechanisms remain largely unknown. The goal of the study was to explore microRNAome alterations induced by ionizing radiation (IR) in murine thymus, and to determine the role of IR-inducible microRNA (miRNA/miR) in the development of leukemia. Methods and Materials: We used the well-established C57BL/6 mouse model and miRNA microarray profiling to identify miRNAs that are differentially expressed in murine thymus in response to irradiation. TIB152 human leukemia cell line was used to determine the role of estrogen receptor–α (ERα) in miR-27b transcription. The biological effects of ectopic miR-27b on leukemogenesis were measured by western immunoblotting, cell viability, apoptosis, and cell cycle analyses. Results: Here, we have shown that IR triggers the differential expression of miR-27b in murine thymus tissue in a dose-, time- and sex-dependent manner. miR-27b was significantly down-regulated in leukemia cell lines CCL119 and TIB152. Interestingly, ERα was overexpressed in those 2 cell lines, and it was inversely correlated with miR-27b expression. Therefore, we used TIB152 as a model system to determine the role of ERα in miR-27b expression and the contribution of miR-27b to leukemogenesis. β-Estradiol caused a rapid and transient reduction in miR-27b expression reversed by either ERα-neutralizing antibody or ERK1/2 inhibitor. Ectopic expression of miR-27b remarkably suppressed TIB152 cell proliferation, at least in part, by inducing S-phase arrest. In addition, it attenuated the expression of cyclin A2, although it had no effect on the levels of PCNA, PPARγ, CDK2, p21, p27, p-p53, and cleaved caspase-3. Conclusion: Our data reveal that β-estradiol/ERα signaling may contribute to the down-regulation of miR-27b in acute leukemia cell lines through the ERK1/2 pathway, and that miR-27b may function as a tumor

  7. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    SciTech Connect (OSTI)

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  8. Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression

    SciTech Connect (OSTI)

    Xiao, Can; Wang, Lili; Zhu, Lifang; Zhang, Chenping; Zhou, Jianhua

    2014-11-28

    Highlights: • miR-9 expression level was significantly decreased in OSCC tissues. • Curcumin significantly inhibited SCC-9 cells proliferation. • miR-9 mediates the inhibition of SCC-9 proliferation by curcumin. • Curcumin suppresses Wnt/β-catenin signaling in SCC-9 cells. • miR-9 mediates the suppression of Wnt/β-catenin signaling by curcumin. - Abstract: Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells, but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future.

  9. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  10. Port Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet)

    U.S. Energy Information Administration (EIA) Indexed Site

    to Canada (Million Cubic Feet) Port Huron, MI Liquefied Natural Gas Exports to Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2013 1 2014 1 1 1 1 2 1 1 1 1 1 2015 1 1 1 1 1 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S. Liquefied Natural Gas Exports by Point of Exit Port Huron, MI Natural Gas Exports to Canad

  11. Post Mortem of 120k mi Light-Duty Urea SCR and DPF System | Department of

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Energy Post Mortem of 120k mi Light-Duty Urea SCR and DPF System Post Mortem of 120k mi Light-Duty Urea SCR and DPF System Presentation given at the 2007 Diesel Engine-Efficiency & Emissions Research Conference (DEER 2007). 13-16 August, 2007, Detroit, Michigan. Sponsored by the U.S. Department of Energy's (DOE) Office of FreedomCAR and Vehicle Technologies (OFCVT). deer07_lambert.pdf (649.68 KB) More Documents & Publications Urea SCR and DPF System for Tier 2 Diesel Light-Duty

  12. MicroRNAs expression in ox-LDL treated HUVECs: MiR-365 modulates apoptosis and Bcl-2 expression

    SciTech Connect (OSTI)

    Qin, Bing; Xiao, Bo; Liang, Desheng; Xia, Jian; Li, Ye; Yang, Huan

    2011-06-24

    Highlights: {yields} We evaluated the role of miRNAs in ox-LDL induced apoptosis in ECs. {yields} We found 4 up-regulated and 11 down-regulated miRNAs in apoptotic ECs. {yields} Target genes of the dysregulated miRNAs regulate ECs apoptosis and atherosclerosis. {yields} MiR-365 promotes ECs apoptosis via suppressing Bcl-2 expression. {yields} MiR-365 inhibitor alleviates ECs apoptosis induced by ox-LDL. -- Abstract: Endothelial cells (ECs) apoptosis induced by oxidized low-density lipoprotein (ox-LDL) is thought to play a critical role in atherosclerosis. MicroRNAs (miRNAs) are a class of noncoding RNAs that posttranscriptionally regulate the expression of genes involved in diverse cell functions, including differentiation, growth, proliferation, and apoptosis. However, whether miRNAs are associated with ox-LDL induced apoptosis and their effect on ECs is still unknown. Therefore, this study evaluated potential miRNAs and their involvement in ECs apoptosis in response to ox-LDL stimulation. Microarray and qRT-PCR analysis performed on human umbilical vein endothelial cells (HUVECs) exposed to ox-LDL identified 15 differentially expressed (4 up- and 11 down-regulated) miRNAs. Web-based query tools were utilized to predict the target genes of the differentially expressed miRNAs, and the potential target genes were classified into different function categories with the gene ontology (GO) term and KEGG pathway annotation. In particular, bioinformatics analysis suggested that anti-apoptotic protein B-cell CLL/lymphoma 2 (Bcl-2) is a target gene of miR-365, an apoptomir up-regulated by ox-LDL stimulation in HUVECs. We further showed that transfection of miR-365 inhibitor partly restored Bcl-2 expression at both mRNA and protein levels, leading to a reduction of ox-LDL-mediated apoptosis in HUVECs. Taken together, our findings indicate that miRNAs participate in ox-LDL-mediated apoptosis in HUVECs. MiR-365 potentiates ox-LDL-induced ECs apoptosis by regulating the

  13. MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ

    SciTech Connect (OSTI)

    Yuan, Jian; Xiao, Gelei; Peng, Gang; Liu, Dingyang; Wang, Zeyou; Liao, Yiwei; Liu, Qing; Wu, Minghua; Yuan, Xianrui

    2015-02-06

    Highlights: • Expression of miR-125a-5p is inversely correlated with that of TAZ in glioma cells. • MiR-125a-5p represses TAZ expression in glioma cells. • MiR-125a-5p directly targets the 3′ UTR of TAZ mRNA and promotes its degradation. • MiR-125a-5p represses CTGF and survivin via TAZ, and inhibits glioma cell growth. • MiR-125a-5p inhibits the stem cell features of HFU-251 MG cells. - Abstract: Glioblastoma (GBM) is the most lethal brain tumor due to the resistance to conventional therapies, such as radiotherapy and chemotherapy. TAZ, an important mediator of the Hippo pathway, was found to be up-regulated in diverse cancers, including in GBM, and plays important roles in tumor initiation and progression. However, little is known about the regulation of TAZ expression in tumors. In this study, we found that miR-125a-5p is an important regulator of TAZ in glioma cells by directly targeting the TAZ 3′ UTR. MiR-125a-5p levels are inversely correlated with that of TAZ in normal astrocytes and a panel of glioma cell lines. MiR-125a-5p represses the expression of TAZ target genes, including CTGF and survivin, and inhibits cell proliferation and induces the differentiation of GBM cells; whereas over-expression of TAZ rescues the effects of miR-125a-5p. This study revealed a mechanism for TAZ deregulation in glioma cells, and also demonstrated a tumor suppressor role of miR-125a-5p in glioblastoma cells.

  14. The NuMI proton beam at Fermilab successes and challenges

    SciTech Connect (OSTI)

    Childress, S.; /Fermilab

    2008-11-01

    The NuMI beam at Fermilab has delivered over 5 x 10{sup 20} 120 GeV protons to the neutrino production target since the start for MINOS [1] neutrino oscillation experiment operation in 2005. We report on proton beam commissioning and operation status, including successes and challenges with this beam.

  15. DOE Zero Energy Ready Home Case Study: Cobblestone Homes, Midland, MI

    Broader source: Energy.gov [DOE]

    Case study of a DOE Zero Energy Ready home in Midland, MI, that scored HERS 49 without PV or HERS 44 with 1.4 kW of PV. The custom home served as a prototype and energy efficiency demonstration...

  16. MiR-18a regulates the proliferation, migration and invasion of human glioblastoma cell by targeting neogenin

    SciTech Connect (OSTI)

    Song, Yichen; Wang, Ping; Zhao, Wei; Yao, Yilong; Liu, Xiaobai; Ma, Jun; Xue, Yixue; Liu, Yunhui

    2014-05-15

    MiR-17-92 cluster has recently been reported as an oncogene in some tumors. However, the association of miR-18a, an important member of this cluster, with glioblastoma remains unknown. Therefore, this study aims to investigate the expression of miR-18a in glioblastoma and its role in biological behavior of U87 and U251 human glioblastoma cell lines. Quantitative RT-PCR results showed that miR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines compared with that in human brain tissues and primary normal human astrocytes, and the expression levels were increased along with the rising pathological grades of glioblastoma. Neogenin was identified as the target gene of miR-18a by dual-luciferase reporter assays. RT-PCR and western blot results showed that its expression levels were decreased along with the rising pathological grades of glioblastoma. Inhibition of miR-18a expression was established by transfecting exogenous miR-18a inhibitor into U87 and U251 cells, and its effects on the biological behavior of glioblastoma cells were studied using CCK-8 assay, transwell assay and flow cytometry. Inhibition of miR-18a expression in U87 and U251 cells significantly up-regulated neogenin, and dramatically suppressed the abilities of cell proliferation, migration and invasion, induced cell cycle arrest and promoted cellular apoptosis. Collectively, these results suggest that miR-18a may regulate biological behavior of human glioblastoma cells by targeting neogenin, and miR-18a can serve as a potential target in the treatment of glioblastoma. - Highlights: • MiR-18a was highly expressed in glioblastoma tissues and U87 and U251 cell lines. • Neogenin was identified as the target gene of miR-18a. • Neogenin expressions were decreased along with the rising pathological grades of glioblastoma. • Inhibition of miR-18a suppressed biological behavior of glioma cells by up-regulating neogenin.

  17. miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer

    SciTech Connect (OSTI)

    Li, Qiaoyan; Zhu, Fufan; Chen, Puxiang

    2012-07-20

    Highlights: Black-Right-Pointing-Pointer Both miR-7 and miR-218 down-regulates HoxB3 expression by targeting the 3 Prime -UTR of HoxB3 mRNA. Black-Right-Pointing-Pointer A reverse correlation between the levels of endogenous miR-7, miR218 and HoxB3 expression. Black-Right-Pointing-Pointer Epigenetic changes involve in the reactivation of HoxB3. Black-Right-Pointing-Pointer Both miRNAs inhibits the cell cycle and clone formation of breast cancer cells. -- Abstract: Many microRNAs have been implicated as key regulators of cellular growth and differentiation and have been found to dysregulate proliferation in human tumors, including breast cancer. Cancer-linked microRNAs also alter the epigenetic landscape by way of DNA methylation and post-translational modifications of histones. Aberrations in Hox gene expression are important for oncogene or tumor suppressor during abnormal development and malignancy. Although recent studies suggest that HoxB3 is critical in breast cancer, the putative role(s) of microRNAs impinging on HoxB3 is not yet fully understood. In this study, we found that the expression levels of miR-7 and miR-218 were strongly and reversely associated with HoxB3 expression. Stable overexpression of miR-7 and miR-218 was accompanied by reactivation of tumor suppressor genes including RASSF1A and Claudin-6 by means of epigenetic switches in DNA methylation and histone modification, giving rise to inhibition of the cell cycle and clone formation of breast cancer cells. The current study provides a novel link between overexpression of collinear Hox genes and multiple microRNAs in human breast malignancy.

  18. Loss of expression of miR-335 is implicated in hepatic stellate cell migration and activation

    SciTech Connect (OSTI)

    Chen, Chao; Wu, Chao-Qun; Zhang, Zong-Qi; Yao, Ding-Kang; Zhu, Liang

    2011-07-15

    Activation and migration of resident stellate cells (HSCs) within the hepatic space of Disse play an important role in hepatic fibrosis, which accounts for the increased numbers of activated HSCs in areas of inflammation during hepatic fibrosis. Currently, microRNAs have been found to play essential roles in HSC differentiation, proliferation, apoptosis, fat accumulation and collagen production. However, little is known about microRNA mediated HSC activation and migration. In this study, the miRNA expression profiles of quiescent HSCs, partially activated HSCs and fully activated HSCs were compared in pairs. Gene ontology (GO) and GO-Map network analysis indicated that the activation of HSCs was regulated by microRNAs. Among them miR-335 was confirmed to be significantly reduced during HSC activation by qRT-PCR, and restoring expression of miR-335 inhibited HSC migration and reduced {alpha}-SMA and collagen type I. Previous study revealed that tenascin-C (TNC), an extracellular matrix glycoprotein involved in cell migration, might be a target of miR-335. Therefore, we further studied the TNC expression in miR-335 over-expressed HSCs. Our data showed that exogenous TNC could enhance HSC migration in vitro and miR-335 restoration resulted in a significant inhibition of TNC expression. These results demonstrated that miR-335 restoration inhibited HSC migration, at least in part, via downregulating the TNC expression.

  19. PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

    SciTech Connect (OSTI)

    Zheng, Peihao; Guo, Honggang; Li, Guangchao; Han, Siqi; Luo, Fei; Liu, Yi

    2015-03-06

    Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.

  20. Building America Case Study: Apartment Compartmentalization with an Aerosol-Based Sealing Process - Queens, NY; Technology Solutions for New and Existing Homes, Energy Efficiency & Renewable Energy (EERE)

    SciTech Connect (OSTI)

    2015-07-01

    Air sealing of building enclosures is a difficult and time-consuming process. Current methods in new construction require laborers to physically locate small and sometimes large holes in multiple assemblies and then manually seal each of them. The innovation demonstrated under this research study was the automated air sealing and compartmentalization of buildings through the use of an aerosolized sealant, developed by the Western Cooling Efficiency Center at University of California Davis.
    CARB sought to demonstrate this new technology application in a multifamily building in Queens, NY. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before and after sealing, point-source testing of individual leaks, and pressure measurements in the walls of the target apartment during sealing. Aerosolized sealing was successful by several measures in this study. Many individual leaks that are labor-intensive to address separately were well sealed by the aerosol particles. In addition, many diffuse leaks that are difficult to identify and treat were also sealed. The aerosol-based sealing process resulted in an average reduction of 71% in air leakage across three apartments and an average apartment airtightness of 0.08 CFM50/SF of enclosure area.

  1. Aryl hydrocarbon receptor-dependent regulation of miR-196a expression controls lung fibroblast apoptosis but not proliferation

    SciTech Connect (OSTI)

    Hecht, Emelia; Zago, Michela; Sarill, Miles; Rico de Souza, Angela; Gomez, Alvin; Matthews, Jason; Hamid, Qutayba; Eidelman, David H.; Baglole, Carolyn J.

    2014-11-01

    The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor implicated in the regulation of apoptosis and proliferation. Although activation of the AhR by xenobiotics such as dioxin inhibits the cell cycle and control apoptosis, paradoxically, AhR expression also promotes cell proliferation and survival independent of exogenous ligands. The microRNA (miRNA) miR-196a has also emerged as a regulator of proliferation and apoptosis but a relationship between the AhR and miR-196a is not known. Therefore, we hypothesized that AhR-dependent regulation of endogenous miR-196a expression would promote cell survival and proliferation. Utilizing lung fibroblasts from AhR deficient (AhR{sup −/−}) and wild-type (AhR{sup +/+}) mice, we show that there is ligand-independent regulation of miRNA, including low miR-196a in AhR{sup −/−} cells. Validation by qRT-PCR revealed a significant decrease in basal expression of miR-196a in AhR{sup −/−} compared to AhR{sup +/+} cells. Exposure to AhR agonists benzo[a]pyrene (B[a]P) and FICZ as well as AhR antagonist CH-223191 decreased miR-196a expression in AhR{sup +/+} fibroblasts concomitant with decreased AhR protein levels. There was increased proliferation only in AhR{sup +/+} lung fibroblasts in response to serum, corresponding to a decrease in p27{sup KIP1} protein, a cyclin-dependent kinase inhibitor. Increasing the cellular levels of miR-196a had no effect on proliferation or expression of p27{sup KIP1} in AhR{sup −/−} fibroblasts but attenuated cigarette smoke-induced apoptosis. This study provides the first evidence that AhR expression is essential for the physiological regulation of cellular miRNA levels- including miR-196a. Future experiments designed to elucidate the functional relationship between the AhR and miR-196a may delineate additional novel ligand-independent roles for the AhR. - Highlights: • The AhR controls proliferation and apoptosis in lung cells. • The AhR regulates the

  2. RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR...

    Office of Scientific and Technical Information (OSTI)

    in prostate cancer Citation Details In-Document Search Title: RLIP76-dependent suppression of PI3KAKTBcl-2 pathway by miR-101 induces apoptosis in prostate cancer MicroRNA-101 ...

  3. Material Activation Benchmark Experiments at the NuMI Hadron Absorber Hall in Fermilab

    SciTech Connect (OSTI)

    Matsumura, H.; Matsuda, N.; Kasugai, Y.; Toyoda, A.; Yashima, H.; Sekimoto, S.; Iwase, H.; Oishi, K.; Sakamoto, Y.; Nakashima, H.; Leveling, A.; Boehnlein, D.; Lauten, G.; Mokhov, N.; Vaziri, K.

    2014-06-15

    In our previous study, double and mirror symmetric activation peaks found for Al and Au arranged spatially on the back of the Hadron absorber of the NuMI beamline in Fermilab were considerably higher than those expected purely from muon-induced reactions. From material activation bench-mark experiments, we conclude that this activation is due to hadrons with energy greater than 3 GeV that had passed downstream through small gaps in the hadron absorber.

  4. Port Huron, MI Natural Gas Pipeline Imports From Canada (Million Cubic

    U.S. Energy Information Administration (EIA) Indexed Site

    Feet) Million Cubic Feet) Port Huron, MI Natural Gas Pipeline Imports From Canada (Million Cubic Feet) Year Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec 2016 262 278 16 - = No Data Reported; -- = Not Applicable; NA = Not Available; W = Withheld to avoid disclosure of individual company data. Release Date: 08/31/2016 Next Release Date: 09/30/2016 Referring Pages: U.S.

  5. Targeting miR-21 enhances the sensitivity of human colon cancer HT-29 cells to chemoradiotherapy in vitro

    SciTech Connect (OSTI)

    Deng, Jun; Lei, Wan; Fu, Jian-Chun; Zhang, Ling; Li, Jun-He; Xiong, Jian-Ping

    2014-01-17

    Highlight: MiR-21 plays a significant role in 5-FU resistance. This role might be attributed to targeting of hMSH2 as well as TP and DPD via miR-21 targeted hMSH2. Indirectly targeted TP and DPD to influence 5-FU chemotherapy sensitivity. -- Abstract: 5-Fluorouracil (5-FU) is a classic chemotherapeutic drug that has been widely used for colorectal cancer treatment, but colorectal cancer cells are often resistant to primary or acquired 5-FU therapy. Several studies have shown that miR-21 is significantly elevated in colorectal cancer. This suggests that this miRNA might play a role in this resistance. In this study, we investigated this possibility and the possible mechanism underlying this role. We showed that forced expression of miR-21 significantly inhibited apoptosis, enhanced cell proliferation, invasion, and colony formation ability, promoted G1/S cell cycle transition and increased the resistance of tumor cells to 5-FU and X radiation in HT-29 colon cancer cells. Furthermore, knockdown of miR-21 reversed these effects on HT-29 cells and increased the sensitivity of HT-29/5-FU to 5-FU chemotherapy. Finally, we showed that miR-21 targeted the human mutS homolog2 (hMSH2), and indirectly regulated the expression of thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD). These results demonstrate that miR-21 may play an important role in the 5-FU resistance of colon cancer cells.

  6. miR-21 modulates tumor outgrowth induced by human adipose tissue-derived mesenchymal stem cells in vivo

    SciTech Connect (OSTI)

    Shin, Keun Koo; Lee, Ae Lim; Kim, Jee Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870; BK21 Medical Science Education Center, School of Medicine, Pusan National University, Yangsan, Gyeongnam 626-870 ; Lee, Sun Young; Medical Research Center for Ischemic Tissue Engineering, Pusan National University, Yangsan, Gyeongnam 626-870 ; Bae, Yong Chan; Jung, Jin Sup

    2012-06-15

    Highlights: Black-Right-Pointing-Pointer miR-21 modulates hADSC-induced increase of tumor growth. Black-Right-Pointing-Pointer The action is mostly mediated by the modulation of TGF-{beta} signaling. Black-Right-Pointing-Pointer Inhibition of miR-21 enhances the blood flow recovery in hindlimb ischemia. -- Abstract: Mesenchymal stem cells (MSCs) have generated a great deal of interest in clinical situations, due principally to their potential use in regenerative medicine and tissue engineering applications. However, the therapeutic application of MSCs remains limited, unless the favorable effects of MSCs on tumor growth in vivo, and the long-term safety of the clinical applications of MSCs, can be more thoroughly understood. In this study, we determined whether microRNAs can modulate MSC-induced tumor outgrowth in BALB/c nude mice. Overexpression of miR-21 in human adipose-derived stem cells (hADSCs) inhibited hADSC-induced tumor growth, and inhibition of miR-21 increased it. Downregulation of transforming growth factor beta receptor II (TGFBR2), but not of signal transducer and activator of transcription 3, in hADSCs showed effects similar to those of miR-21 overexpression. Downregulation of TGFBR2 and overexpression of miR21 decreased tumor vascularity. Inhibition of miR-21 and the addition of TGF-{beta} increased the levels of vascular endothelial growth factor and interleukin-6 in hADSCs. Transplantation of miR-21 inhibitor-transfected hADSCs increased blood flow recovery in a hind limb ischemia model of nude mice, compared with transplantation of control oligo-transfected cells. These findings indicate that MSCs might favor tumor growth in vivo. Thus, it is necessary to study the long-term safety of this technique before MSCs can be used as therapeutic tools in regenerative medicine and tissue engineering.

  7. Measurement of Pi-K Ratios from the NuMI Target

    SciTech Connect (OSTI)

    Seun, Sin Man; /Harvard U.

    2007-07-01

    Interactions of protons (p) with the NuMI (Neutrinos at the Main Injector) target are used to create the neutrino beam for the MINOS (Main Injector Neutrino Oscillation Search) Experiment. Using the MIPP (Main Injector Particle Production) experimental apparatus, the production of charged pions and kaons in p+NuMI interactions is studied. The data come from a sample of 2 x 10{sup 6} events obtained by MIPP using the 120 GeV/c proton beam from the Main Injector at Fermi National Accelerator Laboratory in Illinois, USA. Pions and kaons are identified by measurement in a Ring Imaging Cherenkov detector. Presented are measurements of {pi}{sup -}/{pi}{sup +}, K{sup -}/K{sup +}, {pi}{sup +}/K{sup +} and {pi}{sup -}/K{sup -} production ratios in the momentum range p{sub T} < 2 GeV/c transversely and 20 GeV/c < p{sub z} < 90 GeV/c longitudinally. Also provided are detailed comparisons of the MIPP NuMI data with the MIPP Thin Carbon data, the MIPP Monte Carlo simulation and the current MINOS models in the relevant momentum ranges.

  8. miR-502 inhibits cell proliferation and tumor growth in hepatocellular carcinoma through suppressing phosphoinositide 3-kinase catalytic subunit gamma

    SciTech Connect (OSTI)

    Chen, Suling; Li, Fang; Chai, Haiyun; Tao, Xin; Wang, Haili; Ji, Aifang

    2015-08-21

    MicroRNAs (miRNAs) play a key role in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). In the present study, we demonstrated that miR-502 significantly inhibits HCC cell proliferation in vitro and tumor growth in vivo. G1/S cell cycle arrest and apoptosis of HCC cells were induced by miR-502. Phosphoinositide 3-kinase catalytic subunit gamma (PIK3CG) was identified as a direct downstream target of miR-502 in HCC cells. Notably, overexpression of PIK3CG reversed the inhibitory effects of miR-502 in HCC cells. Our findings suggest that miR-502 functions as a tumor suppressor in HCC via inhibition of PI3KCG, supporting its utility as a promising therapeutic gene target for this tumor type. - Highlights: • miR-502 suppresses HCC cell proliferation in vitro and tumorigenicity in vivo. • miR-502 regulates cell cycle and apoptosis in HCC cells. • PIK3CG is a direct target of miR-502. • miR-502 and PIK3CG expression patterns are inversely correlated in HCC tissues.

  9. MiR-145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1

    SciTech Connect (OSTI)

    Wu, Huijuan; Xiao, ZhengHua; Wang, Ke; Liu, Wenxin; Hao, Quan

    2013-11-29

    Highlights: MiR-145 is downregulated in human ovarian cancer. MiR-145 targets p70S6K1 and MUC1. p70S6K1 and MUC1 are involved in miR-145 mediated tumor cell growth and cell invasion, respectively. -- Abstract: MicroRNAs (miRNAs) are a family of small non-coding RNA molecules that regulate gene expression at post-transcriptional levels. Previous studies have shown that miR-145 is downregulated in human ovarian cancer; however, the roles of miR-145 in ovarian cancer growth and invasion have not been fully demonstrated. In the present study, Northern blot and qRT-PCR analysis indicate that miR-145 is downregulated in ovarian cancer tissues and cell lines, as well as in serum samples of ovarian cancer, compared to healthy ovarian tissues, cell lines and serum samples. Functional studies suggest that miR-145 overexpression leads to the inhibition of colony formation, cell proliferation, cell growth viability and invasion, and the induction of cell apoptosis. In accordance with the effect of miR-145 on cell growth, miR-145 suppresses tumor growth in vivo. MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3?UTRs. Importantly, the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145, respectively. MiR-145 expression is increased after 5-aza-CdR treatment, and 5-aza-CdR treatment results in the same phenotype as the effect of miR-145 overexpression. Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1, functioning as a tumor suppressor. Moreover, our data imply that miR-145 has potential as a miRNA-based therapeutic target for ovarian cancer.

  10. Testing CPT conservation using the NuMI neutrino beam with the MINOS experiment

    SciTech Connect (OSTI)

    Auty, David John

    2010-05-01

    The MINOS experiment was designed to measure neutrino oscillation parameters with muon neutrinos. It achieves this by measuring the neutrino energy spectrum and flavor composition of the man-made NuMI neutrino beam 1km after the beam is formed and again after 735 km. By comparing the two spectra it is possible to measure the oscillation parameters. The NuMI beam is made up of 7.0% {bar {nu}}{sub {mu}}, which can be separated from the {nu}{sub {mu}} because the MINOS detectors are magnetized. This makes it possible to study {bar {nu}}{sub {mu}} oscillations separately from those of muon neutrinos, and thereby test CPT invariance in the neutrino sector by determining the {bar {nu}}{sub {mu}} oscillation parameters and comparing them with those for {nu}{sub {mu}}, although any unknown physics of the antineutrino would appear as a difference in oscillation parameters. Such a test has not been performed with beam {bar {nu}}{sub {mu}} before. It is also possible to produce an almost pure {bar {nu}}{sub {mu}} beam by reversing the current through the magnetic focusing horns of the NuMI beamline, thereby focusing negatively, instead of positively charged particles. This thesis describes the analysis of the 7% {bar {nu}}{sub {mu}} component of the forward horn current NuMI beam. The {bar {nu}}{sub {mu}} of a data sample of 3.2 x 10{sup 20} protons on target analysis found 42 events, compared to a CPT conserving prediction of 58.3{sub -7.6}{sup +7.6}(stat.){sub -3.6}{sup +3.6}(syst.) events. This corresponds to a 1.9 {sigma} deficit, and a best fit value of {Delta}{bar m}{sub 32}{sup 2} = 18 x 10{sup -3} eV{sup 2} and sin{sup 2} 2{bar {theta}}{sub 23} = 0.55. This thesis focuses particularly on the selection of {bar {nu}}{sub {mu}} events, and investigates possible improvements of the selection algorithm. From this a different selector was chosen, which corroborated the findings of the original selector. The thesis also investigates how the systematic errors affect the