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Sample records for manager coconino nf

  1. EA-1863: Vegetation Management on the Glen Canyon-Pinnacle Peak Transmission Lines Spanning the Coconino National Forest, Coconino County, Arizona

    Broader source: Energy.gov [DOE]

    DOE’s Western Area Power Administration is preparing this EA to evaluate the environmental impacts of updating the vegetation management and right-of-way maintenance program for Western’s Glen Canyon to Pinnacle Peak 345-kV transmission lines, which cross the Coconino National Forest, Coconino County, Arizona.

  2. Property:SurfaceManager | Open Energy Information

    Open Energy Info (EERE)

    + COCONINO NF + AZA-009169 + COCONINO NF + AZA-009170 + COCONINO NF + AZA-009171 + FOREST SERVICE + AZA-009172 + FOREST SERVICE + AZA-009173 + FOREST SERVICE + AZA-009174 +...

  3. EIS-0427: Grapevine Canyon Wind Project, Coconino County, Arizona

    Broader source: Energy.gov [DOE]

    This EIS evaluates the environmental impacts of a proposed wind energy generation project in Coconino County, Arizona, on privately owned ranch lands and trust lands administered by the Arizona State Land Department. The proposed project includes a new transmission tie-line that would cross lands administered by Coconino National Forest and interconnect with DOE’s Western Area Power Administration’s existing Glen Canyon-Pinnacle Peak transmission lines.

  4. Fossil vertebrate footprints in the Coconino Sandstone (Permian) of northern Arizona: Evidence for underwater origin

    SciTech Connect (OSTI)

    Brand, L.R.; Thu Tang (Loma Linda Univ., CA (United States))

    1991-12-01

    Numerous fossil vertebrate trackways in the Coconino Sandstone of northern Arizona exhibit several features that imply that these trackways were not made in subaerial conditions. Some trackways begin or end abruptly on undisturbed bedding planes, and in other trackways the individual prints are oriented in a different direction from that of the trackway. These features indicate buoyancy of the animals in water. The animals were swimming in the water part of the time and at other times walking on the substrate, and they were sometimes orienting upslope on the surface of the underwater dunes, while being drifted sideways by lateral currents. Observations on salamander locomotion in a sedimentation tank with flowing water support this model.

  5. AZA-009191 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT OIL USA INC Lessee 2 none provided Effective Date Expire Date Held By Production (HBP) HBP...

  6. AZA-009190 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT OIL USA INC Lessee 2 none provided Effective Date Expire Date Held By Production (HBP) HBP...

  7. AZA-009186 | Open Energy Information

    Open Energy Info (EERE)

    PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT OIL USA INC Lessee 2 none provided Effective Date Expire Date Held By Production (HBP) HBP...

  8. AZA-009169 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 2560 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  9. AZA-009170 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 1920 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  10. AZA-009168 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 2005 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  11. AZA-009175 | Open Energy Information

    Open Energy Info (EERE)

    (40 Acres) Case Status Closed Case Type GEOLSENONCOMPPRE2005 Total Acreage 2190 Bid PriceAcre Managing Field Office none provided Surface Manager COCONINO NF Lessee 1 HUNT W H...

  12. ARM - VAP Product - aerich2nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Productsaerinfaerich2nf1turn Documentation Data Management Facility Plots (Quick Looks) Citation DOI: 10.54391027273 What is this? Generate Citation ARM Data Discovery Browse...

  13. ARM - VAP Product - aerich1nf1turn

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Productsaerinfaerich1nf1turn Documentation Data Management Facility Plots (Quick Looks) Citation DOI: 10.54391027272 What is this? Generate Citation ARM Data Discovery Browse...

  14. NF_Eder_2013

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    24 October 2013 Online at stacks.iop.orgNF53113037 Abstract Modelling and mitigation of damage are crucial for safe and economical operation of high-power laser facilities. ...

  15. NF Energy Saving Corp | Open Energy Information

    Open Energy Info (EERE)

    NF Energy Saving Corp Jump to: navigation, search Name: NF Energy Saving Corp Place: Shenyang, Liaoning Province, China Sector: Services Product: China-based company provides...

  16. EA-1863: Final Environmental Assessment

    Broader source: Energy.gov [DOE]

    Vegetation Management Plan for Glen Canyon to Pinnacle Peak 345-kV transmission line, Coconino National Forest, Arizona

  17. EA-1863: Finding of No Significant Impact

    Broader source: Energy.gov [DOE]

    Vegetation Management Plan for Glen Canyon to Pinnacle Peak 345-kV transmission line, Coconino National Forest, Arizona

  18. EA-2008: Colorado River Storage Project Programmatic Operations and Maintenance Project; Coconino, Maricopa, Navajo, and Yavapai Counties (Arizona) and San Juan County (Utah)

    Broader source: Energy.gov [DOE]

    Western Area Power Administration is preparing an EA that assesses the potential environmental impacts of Western’s programmatic operations and management (O&M) process and an integrated vegetation management (IVM) program on the Colorado River Storage Project System. O&M activities would consist of aerial and ground patrols, regular and preventive maintenance, inspections and repairs, and road repair. The IVM program would remove vegetation to protect facilities from fire, control the spread of noxious weeds to protect environmental quality, establish and maintain stable, low-growing plant communities in the ROW, and activities for public and worker safety around transmission lines and other facilities.

  19. DOE/EA-2008: Colorado River Storage Project Programmatic Operations and Maintenance Project; Coconino, Maricopa, Navajo, and Yavapai Counties (Arizona) and San Juan County (Utah)

    Broader source: Energy.gov [DOE]

    Western Area Power Administration is preparing an EA that assesses the potential environmental impacts of Westerns programmatic operations and management (O&M) process and an integrated vegetation management (IVM) program on the Colorado River Storage Project System. O&M activities would consist of aerial and ground patrols, regular and preventive maintenance, inspections and repairs, and road repair. The IVM program would remove vegetation to protect facilities from fire, control the spread of noxious weeds to protect environmental quality, establish and maintain stable, low-growing plant communities in the ROW, and activities for public and worker safety around transmission lines and other facilities.

  20. CMRR-NF Supplemental EIS Scoping Comments | National Nuclear Security

    National Nuclear Security Administration (NNSA)

    Administration | (NNSA) CMRR-NF Supplemental EIS Scoping Comments CMRR-NF Supplemental EIS Scoping Comments Additional CMRR Scoping Comments - 47A Additional CMRR Scoping Comments - 48A Additional CMRR Scoping Comments - 62 Additional CMRR Scoping Comments - 63 Additional CMRR Scoping Comments - 64 Additional CMRR Scoping Comments - 66A Additional CMRR Scoping Comments - 66B Additional CMRR Scoping Comments - 66C Additional CMRR Scoping Comments - 76 Additional CMRR Scoping Comments - 77

  1. management

    National Nuclear Security Administration (NNSA)

    5%2A en Management and Budget http:www.nnsa.energy.govaboutusouroperationsmanagementandbudget

  2. management

    National Nuclear Security Administration (NNSA)

    5%2A en Management and Budget http:nnsa.energy.govaboutusouroperationsmanagementandbudget

    P...

  3. EA-1863: Draft Environmental Assessment

    Broader source: Energy.gov [DOE]

    Glen Canyon to Pinnacle Peak 345 kV Transmission Lines Vegetation Management Project within the Coconino National Forest, Flagstaff, Arizona

  4. IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    IDS-NF Impact of Neutrino Cross Section Impact of Neutrino Cross Section Knowledge on Oscillation Knowledge on Oscillation Measurements Measurements M. Sorel, IFIC (CSIC and U. of Valencia) IDS-NF, RAL, Jan 16-17 2008 M. Sorel - IFIC (Valencia U. & CSIC) 2 IDS-NF Neutrino Cross Sections: At What Energies Needed? Superbeams: Solid: T2K Dashed: NovA M. Sorel - IFIC (Valencia U. & CSIC) 3 IDS-NF Neutrino Cross Sections: At What Energies Needed? Superbeams: Solid: T2K Dashed: NovA Beta

  5. Smoothing single-crystalline SiC surfaces by reactive ion etching using pure NF{sub 3} and NF{sub 3}/Ar mixture gas plasmas

    SciTech Connect (OSTI)

    Tasaka, Akimasa; Kotaka, Yuki; Oda, Atsushi; Saito, Morihiro; Tojo, Tetsuro; Inaba, Minoru

    2014-09-01

    In pure NF{sub 3} plasma, the etching rates of four kinds of single-crystalline SiC wafer etched at NF{sub 3} pressure of 2 Pa were the highest and it decreased with an increase in NF{sub 3} pressure. On the other hand, they increased with an increase in radio frequency (RF) power and were the highest at RF power of 200 W. A smooth surface was obtained on the single-crystalline 4H-SiC after reactive ion etching at NF{sub 3}/Ar gas pressure of 2 Pa and addition of Ar to NF{sub 3} plasma increased the smoothness of SiC surface. Scanning electron microscopy observation revealed that the number of pillars decreased with an increase in the Ar-concentration in the NF{sub 3}/Ar mixture gas. The roughness factor (R{sub a}) values were decreased from 51.5 nm to 25.5 nm for the As-cut SiC, from 0.25 nm to 0.20 nm for the Epi-SiC, from 5.0 nm to 0.7 nm for the Si-face mirror-polished SiC, and from 0.20 nm to 0.16 nm for the C-face mirror-polished SiC by adding 60% Ar to the NF{sub 3} gas. Both the R{sub a} values of the Epi- and the C-face mirror-polished wafer surfaces etched using the NF{sub 3}/Ar (40:60) plasma were similar to that treated with mirror polishing, so-called the Catalyst-Referred Etching (CARE) method, with which the lowest roughness of surface was obtained among the chemical mirror polishing methods. Etching duration for smoothing the single-crystalline SiC surface using its treatment was one third of that with the CARE method.

  6. Coconino County, Arizona: Energy Resources | Open Energy Information

    Open Energy Info (EERE)

    Sedona, Arizona Supai, Arizona Tonalea, Arizona Tuba City, Arizona Tusayan, Arizona Williams, Arizona Winslow West, Arizona Retrieved from "http:en.openei.orgw...

  7. Smad7 mediates inhibition of Saos2 osteosarcoma cell differentiation by NF{kappa}B

    SciTech Connect (OSTI)

    Eliseev, Roman A. . E-mail: Roman_Eliseev@urmc.rochester.edu; Schwarz, Edward M.; Zuscik, Michael J.; O'Keefe, Regis J.; Drissi, Hicham; Rosier, Randy N.

    2006-01-01

    The transcription factor NF{kappa}B is constitutively activated in various tumor cells where it promotes proliferation and represses apoptosis. The bone morphogenetic proteins (BMPs) delay cell proliferation and promote differentiation and apoptosis of bone cells through activation of Smad downstream effectors and via Smad-independent mechanisms. Thus, NF{kappa}B and BMP pathways play opposing roles in regulating osteoblastic cell fate. Here, we show that in osteosarcoma Saos2 osteoblasts, NF{kappa}B regulates the activity of the BMP/Smad signaling. Inhibition of NF{kappa}B by overexpression of mI{kappa}B leads to the induction of osteoblast differentiation. Saos2 cells overexpressing mI{kappa}B (Saos2-mI{kappa}B) exhibit higher expression of osteoblast phenotypic genes such as alkaline phosphatase, Runx2 and osteocalcin and are more responsive to BMP2 in comparison to wild-type cells (Saos2-wt) or empty vector infected controls (Saos2-EV). Furthermore, BMP-2 signaling and Smad phosphorylation are significantly increased in Saos2-mI{kappa}B cells in comparison to Saos2-EV cells. Inhibition of NF{kappa}B signaling in Saos2-mI{kappa}B cells is associated with decreased expression of the BMP signaling inhibitor Smad7. While gain of Smad7 function in Saos2-mI{kappa}B cells results in inhibition of BMP signaling, anti-sense knockdown of Smad7 in Saos2-EV cells leads to upregulation of BMP signaling. We therefore conclude that in osteosarcoma Saos2 cells, NF{kappa}B represses BMP/Smad signaling and BMP2-induced differentiation through Smad7.

  8. Regulation Of Nf=kb And Mnsod In Low Dose Radiation Induced Adaptive Protection Of Mouse And Human Skin Cells

    SciTech Connect (OSTI)

    Jian Li

    2012-11-07

    A sampling of publications resulting from this grant is provided. One is on the subject of NF-κB-Mediated HER2 Overexpression in Radiation-Adaptive Resistance. Another is on NF-κB-mediated adaptive resistance to ionizing radiation.

  9. OSU-A9 inhibits angiogenesis in human umbilical vein endothelial cells via disrupting AktNF-?B and MAPK signaling pathways

    SciTech Connect (OSTI)

    Omar, Hany A.; Arafa, El-Shaimaa A.; Salama, Samir A.; Arab, Hany H.; Wu, Chieh-Hsi; Weng, Jing-Ru

    2013-11-01

    Since the introduction of angiogenesis as a useful target for cancer therapy, few agents have been approved for clinical use due to the rapid development of resistance. This problem can be minimized by simultaneous targeting of multiple angiogenesis signaling pathways, a potential strategy in cancer management known as polypharmacology. The current study aimed at exploring the anti-angiogenic activity of OSU-A9, an indole-3-carbinol-derived pleotropic agent that targets mainly Aktnuclear factor-kappa B (NF-?B) signaling which regulates many key players of angiogenesis such as vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Human umbilical vein endothelial cells (HUVECs) were used to study the in vitro anti-angiogenic effect of OSU-A9 on several key steps of angiogenesis. Results showed that OSU-A9 effectively inhibited cell proliferation and induced apoptosis and cell cycle arrest in HUVECs. Besides, OSU-A9 inhibited angiogenesis as evidenced by abrogation of migration/invasion and Matrigel tube formation in HUVECs and attenuation of the in vivo neovascularization in the chicken chorioallantoic membrane assay. Mechanistically, Western blot, RT-PCR and ELISA analyses showed the ability of OSU-A9 to inhibit MMP-2 production and VEGF expression induced by hypoxia or phorbol-12-myristyl-13-acetate. Furthermore, dual inhibition of AktNF-?B and mitogen-activated protein kinase (MAPK) signaling, the key regulators of angiogenesis, was observed. Together, the current study highlights evidences for the promising anti-angiogenic activity of OSU-A9, at least in part through the inhibition of AktNF-?B and MAPK signaling and their consequent inhibition of VEGF and MMP-2. These findings support OSU-A9's clinical promise as a component of anticancer therapy. - Highlights: The antiangiogenic activity of OSU-A9 in HUVECs was explored. OSU-A9 inhibited HUVECs proliferation, migration, invasion and tube formation. OSU-A9 targeted signaling

  10. Withaferin A disrupts ubiquitin-based NEMO reorganization induced by canonical NF-κB signaling

    SciTech Connect (OSTI)

    Jackson, Shawn S.; Oberley, Christopher; Hooper, Christopher P.; Grindle, Kreg; Wuerzberger-Davis, Shelly; Wolff, Jared; and others

    2015-02-01

    The NF-κB family of transcription factors regulates numerous cellular processes, including cell proliferation and survival responses. The constitutive activation of NF-κB has also emerged as an important oncogenic driver in many malignancies, such as activated B-cell like diffuse large B cell lymphoma, among others. In this study, we investigated the impact and mechanisms of action of Withaferin A, a naturally produced steroidal lactone, against both signal-inducible as well as constitutive NF-κB activities. We found that Withaferin A is a robust inhibitor of canonical and constitutive NF-κB activities, leading to apoptosis of certain lymphoma lines. In the canonical pathway induced by TNF, Withaferin A did not disrupt RIP1 polyubiquitination or NEMO–IKKβ interaction and was a poor direct IKKβ inhibitor, but prevented the formation of TNF-induced NEMO foci which colocalized with TNF ligand. While GFP-NEMO efficiently formed TNF-induced foci, a GFP-NEMO{sup Y308S} mutant that is defective in binding to polyubiquitin chains did not form foci. Our study reveals that Withaferin A is a novel type of IKK inhibitor which acts by disrupting NEMO reorganization into ubiquitin-based signaling structures in vivo. - Highlights: • Withaferin A, a NF-κB inhibitor, disrupts signaling induced NEMO localization, a novel point of inhibition. • NEMO can be localized to distinct signaling foci after treatment with TNF. • ABC-type DLCBL cells can be sensitized to apoptosis after treatment with Withaferin A.

  11. Piperlongumine selectively suppresses ABC-DLBCL through inhibition of NF-κB p65 subunit nuclear import

    SciTech Connect (OSTI)

    Niu, Mingshan; Shen, Yangling; Xu, Xiaoyu; Yao, Yao; Fu, Chunling; Yan, Zhiling; Wu, Qingyun; Cao, Jiang; Sang, Wei; Zeng, Lingyu; Li, Zhenyu; Liu, Xuejiao; and others

    2015-07-10

    Constitutive NF-κB activation is required for survival of activated B cell-like subtype of diffuse large B cell lymphoma (ABC-DLBCL). However, current NF-κB targeting strategies lack cancer cell specificity. Here, we identified a novel inhibitor, piperlongumine, features direct binding to NF-κB p65 subunit and suppression of p65 nuclear import. This was accompanied by NF-κB reporter activity suppression and NF-κB target gene downregulation. Moreover, mutation of Cys{sup 38} to Ser in p65 abolished this effect of piperlongumine on inhibition of p65 nuclear import. Furthermore, we show that piperlongumine selectively inhibited proliferation and induced apoptosis of ABC-DLBCL cells. Most notably, it has been reported that piperlongumine did not affect normal cells even at high doses and was nontoxic to animals. Hence, our current study provides new insight into piperlongumine's mechanism of action and novel approach to ABC-DLBCL target therapy. - Highlights: • Current NF-κB targeting strategies lack cancer cell specificity. • Piperlongumine inhibits NF-κB p65 subunit nuclear import via directly binding to p65. • Piperlongumine selectively inhibits proliferation of ABC-DLBCL cells. • This study provides a novel approach to ABC-DLBCL target therapy.

  12. Coal dust contiguity-induced changes in the concentration of TNF- and NF- B p65 on the ocular surface

    SciTech Connect (OSTI)

    Sun, Z.Y.; Hong, J.; Liu, Z.Y.; Jin, X.D.; Gu, C.H.

    2009-07-01

    To observe the influence of coal dust on ocular surface of coal miners and rabbits with coal dust contiguity on expression TNF- and NF- Bp65 and dry eye occurrence. Expression TNF- and NF- Bp65 in ocular surface were determined. Results showed tear production, BUT and lysozyme decreased for coal miners and rabbits with coal dust contiguity. Coal dust exposure was linked to development of xerophthalmia, and induced a higher expression of NF- B p65 and TNF- perhaps as a mechanism to resist coal dust ocular surface injury.

  13. BRAF activated non-coding RNA (BANCR) promoting gastric cancer cells proliferation via regulation of NF-κB1

    SciTech Connect (OSTI)

    Zhang, Zhi-Xin; Liu, Zhi-Qiang; Jiang, Biao; Lu, Xin-Yang; Ning, Xiao-Fei; Yuan, Chuan-Tao; Wang, Ai-Liang

    2015-09-18

    Background and objective: Long non-coding RNA, BANCR, has been demonstrated to contribute to the proliferation and migration of tumors. However, its molecular mechanism underlying gastric cancer is still unknown. In present study, we investigated whether BANCR was involved in the development of gastric cancer cells via regulation of NF-κB1. Methods: Human gastric cancer tissues were isolated as well as human gastric cell lines MGC803 and BGC823 were cultured to investigate the role of BANCR in gastric cancer. Results: BANCR expression was significantly up-regulated in gastric tumor tissues and gastric cell lines. Down-regulation of BANCR inhibited gastric cancer cell growth and promoted cell apoptosis, and it also contributed to a significant decrease of NF-κB1 (P50/105) expression and 3′UTR of NF-κB1 activity. Overexpression of NF-κB1 reversed the effect of BANCR on cancer cell growth and apoptosis. MiroRNA-9 (miR-9) targeted NF-κB1, and miR-9 inhibitor also reversed the effects of BANCR on gastric cancer cell growth and apoptosis. Conclusion: BANCR was highly expressed both in gastric tumor tissues and in cancer cells. NF-κB1 and miR-9 were involved in the role of BANCR in gastric cancer cell growth and apoptosis. - Highlights: • BANCR up-regulated in gastric cancer (GC) tissues and cell lines MGC803 and BGC823. • Down-regulation of BANCR inhibited GC cell growth and promoted cell apoptosis. • Down-regulation of BANCR contributed to decreased 3′UTR of NF-κB1 and its expression. • Overexpressed NF-κB1 reversed the effect of BANCR on GC cell growth. • miR-9 inhibitor reversed the effect of BANCR on cancer GC cell growth.

  14. XEDAR activates the non-canonical NF-κB pathway

    SciTech Connect (OSTI)

    Verhelst, Kelly; Gardam, Sandra; Borghi, Alice; Kreike, Marja; Carpentier, Isabelle; Beyaert, Rudi

    2015-09-18

    Members of the tumor necrosis factor receptor (TNFR) superfamily are involved in a number of physiological and pathological responses by activating a wide variety of intracellular signaling pathways. The X-linked ectodermal dysplasia receptor (XEDAR; also known as EDA2R or TNFRSF27) is a member of the TNFR superfamily that is highly expressed in ectodermal derivatives during embryonic development and binds to ectodysplasin-A2 (EDA-A2), a member of the TNF family that is encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Although XEDAR was first described in the year 2000, its function and molecular mechanism of action is still largely unclear. XEDAR has been reported to activate canonical nuclear factor κB (NF-κB) signaling and mitogen-activated protein (MAP) kinases. Here we report that XEDAR is also able to trigger the non-canonical NF-κB pathway, characterized by the processing of p100 (NF-κB2) into p52, followed by nuclear translocation of p52 and RelB. We provide evidence that XEDAR-induced p100 processing relies on the binding of XEDAR to TRAF3 and TRAF6, and requires the kinase activity of NIK and IKKα. We also show that XEDAR stimulation results in NIK accumulation and that p100 processing is negatively regulated by TRAF3, cIAP1 and A20. - Highlights: • XEDAR activates the non-canonical NF-κB pathway. • XEDAR-induced processing of p100 depends on XEDAR interaction with TRAF3 and TRAF6. • XEDAR-induced processing of p100 depends on NIK and IKKα activity. • Overexpression of XEDAR leads to NIK accumulation. • XEDAR-induced processing of p100 is negatively regulated by TRAF3 cIAP1 and A20.

  15. Agreement Execution Process Study: CRADAs and NF-WFO Agreements and the Speed of Business

    SciTech Connect (OSTI)

    Harrer, Bruce J.; Cejka, Cheryl L.; Macklin, Richard; Miksovic, Ann

    2011-02-01

    This report summarizes the findings of a study on the execution of Cooperative Research and Development Agreements (CRADAs) and Non-Federal Work for Others (NF-WFO) agreements across the U.S. Department of Energy (DOE) laboratory complex. The study provides quantitiative estimates of times required to negotiate and execute these agreements across the DOE complex. It identifies factors impacting on cycle times and describes best practicies used at various laboratories and site offices that reduce cycle times.

  16. Characterization of the Kinetics of NF3-Fluorination of NpO2

    SciTech Connect (OSTI)

    Casella, Andrew M.; Scheele, Randall D.; McNamara, Bruce K.

    2015-12-23

    The exploitation of selected actinide and fission product fluoride volatilities has long been considered as a potentially attractive compact method for recycling used nuclear fuels to avoid generating the large volumes of radioactive waste arising from aqueous reprocessing [1-7]. The most developed process uses the aggressive and hazardous fluorinating agents hydrogen fluoride (HF) and/or molecular fluorine (F2) at high temperatures to volatilize the greatest fraction of the used nuclear fuel into a single gas stream. The volatilized fluorides are subsequently separated using a series of fractionation and condensation columns to recover the valuable fuel constituents and fission products. In pursuit of a safer and less complicated approach, we investigated an alternative fluoride volatility-based process using the less hazardous fluorinating agent nitrogen trifluoride (NF3) and leveraging its less aggressive nature to selectively evolve fission product and actinide fluorides from the solid phase based on their reaction temperatures into a single recycle stream [8-15]. In this approach, successive isothermal treatments using NF3 will first evolve the more thermally susceptible used nuclear fuel constituents leaving the other constituents in the residual solids until subsequent isothermal temperature treatments cause these others to volatilize. During investigation of this process, individual neat used fuel components were treated with isothermal NF3 in an attempt to characterize the kinetics of each fluorination reaction to provide input into the design of a new volatile fluoride separations approach. In these directed investigations, complex behavior was observed between NF3 and certain solid reactants such as the actinide oxides of uranium, plutonium, and neptunium. Given the similar thermal reaction susceptibilities of neptunium oxide (NpO2) and uranium dioxide (UO2) and the importance of Np and U, we initially focused our efforts on determining the reaction

  17. Nickel differentially regulates NFAT and NF-{kappa}B activation in T cell signaling

    SciTech Connect (OSTI)

    Saito, Rumiko; Hirakawa, Satoshi; Ohara, Hiroshi; Yasuda, Makoto; Yamazaki, Tomomi; Nishii, Shigeaki; Aiba, Setsuya

    2011-08-01

    Nickel is a potent hapten that induces contact hypersensitivity in human skin. While nickel induces the maturation of dendritic cells via NF-{kappa}B and p38 MAPK activation, it also exerts immunosuppressive effects on T cells through an unknown mechanism. To elucidate the molecular mechanisms of its effects on T cells, we examined the effects of NiCl{sub 2} on mRNA expression in human CD3+ T cells stimulated with CD3 and CD28 antibodies. Using a DNA microarray and Gene Ontology, we identified 70 up-regulated (including IL-1{beta}, IL-6 and IL-8) and 61 down-regulated (including IL-2, IL-4, IL-10 and IFN-{gamma}) immune responsive genes in NiCl{sub 2}-treated T cells. The DNA microarray results were verified using real-time PCR and a Bio-Plex{sup TM} suspension protein array. Suppression of IL-2 and IFN-{gamma} gene transcription by NiCl{sub 2} was also confirmed using Jurkat T cells transfected with IL-2 or IFN-{gamma} luciferase reporter genes. To explore the NiCl{sub 2}-regulated signaling pathway, we examined the binding activity of nuclear proteins to NFAT, AP-1, and NF-{kappa}B consensus sequences. NiCl{sub 2} significantly and dose-dependently suppressed NFAT- and AP-1-binding activity, but augmented NF-{kappa}B-binding activity. Moreover, NiCl{sub 2} decreased nuclear NFAT expression in stimulated T cells. Using Jurkat T cells stimulated with PMA/ionomycin, we demonstrated that NiCl{sub 2} significantly suppressed stimulation-evoked cytosolic Ca{sup 2+} increases, suggesting that NiCl{sub 2} regulates NFAT signals by acting as a blocker of Ca{sup 2+} release-activated Ca{sup 2+} (CRAC) channels. These data showed that NiCl{sub 2} decreases NFAT and increases NF-{kappa}B signaling in T cells. These results shed light on the effects of nickel on the molecular regulation of T cell signaling. - Graphical Abstract: Nickel suppresses stimulation-evoked cytosolic Ca{sup 2+} increase, which results in the suppression of NFAT signals. On the other hand, Ni rather

  18. Constitutive NF-?B activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production

    SciTech Connect (OSTI)

    Chung, Jin Sil; Lee, Sora; Yoo, Young Do

    2014-08-08

    Highlights: Romo1 expression is required for constitutive nuclear DNA-binding activity of NF-?B. Romo1 depletion suppresses tumor growth in vivo. Romo1 presents a potential therapeutic target for diseases. - Abstract: Deregulation of nuclear factor-?B (NF-?B) and related pathways contribute to tumor cell proliferation and invasion. Mechanisms for constitutive NF-?B activation are not fully explained; however, the underlying defects appear to generate and maintain pro-oxidative conditions. In hepatocellular carcinoma (HCC) tissues, up-regulation of reactive oxygen species modulator 1 (Romo1) correlates positively with tumor size. In the present study, we showed that Romo1 expression is required to maintain constitutive nuclear DNA-binding activity of NF-?B and transcriptional activity through constitutive I?B? phosphorylation. Overexpression of Romo1 promoted p65 nuclear translocation and DNA-binding activity. We also show that Romo1 depletion suppressed anchorage-independent colony formation by HCC cells and suppressed tumor growth in vivo. Based on these findings, Romo1 may be a principal regulatory factor in the maintenance of constitutive NF-?B activation in tumor cells. In the interest of anti-proliferative treatments for cancer, Romo1 may also present a productive target for drug development.

  19. XIAP Induces NF-kB Activation via the BIR1/TAB1 Interaction and BIR1 Dimerization

    SciTech Connect (OSTI)

    Lu,M.; Lin, S.; Huang, Y.; Kang, Y.; Rich, R.; Lo, Y.; Myszka, D.; Han, J.; Wu, H.

    2007-01-01

    In addition to caspase inhibition, X-linked inhibitor of apoptosis (XIAP) induces NF-{kappa}B and MAP kinase activation during TGF-b and BMP receptor signaling and upon overexpression. Here we show that the BIR1 domain of XIAP, which has no previously ascribed function, directly interacts with TAB1 to induce NF-{kappa}B activation. TAB1 is an upstream adaptor for the activation of the kinase TAK1, which in turn couples to the NF-{kappa}B pathway. We report the crystal structures of BIR1, TAB1, and the BIR1/TAB1 complex. The BIR1/TAB1 structure reveals a striking butterfly-shaped dimer and the detailed interaction between BIR1 and TAB1. Structure-based mutagenesis and knockdown of TAB1 show unambiguously that the BIR1/TAB1 interaction is crucial for XIAP-induced TAK1 and NF-{kappa}B activation. We show that although not interacting with BIR1, Smac, the antagonist for caspase inhibition by XIAP, also inhibits the XIAP/TAB1 interaction. Disruption of BIR1 dimerization abolishes XIAP-mediated NF-{kappa}B activation, implicating a proximity-induced mechanism for TAK1 activation.

  20. FY13 Summary Report on the Augmentation of the Spent Fuel Composition Dataset for Nuclear Forensics: SFCOMPO/NF

    SciTech Connect (OSTI)

    Brady Raap, Michaele C.; Lyons, Jennifer A.; Collins, Brian A.; Livingston, James V.

    2014-03-31

    This report documents the FY13 efforts to enhance a dataset of spent nuclear fuel isotopic composition data for use in developing intrinsic signatures for nuclear forensics. A review and collection of data from the open literature was performed in FY10. In FY11, the Spent Fuel COMPOsition (SFCOMPO) excel-based dataset for nuclear forensics (NF), SFCOMPO/NF was established and measured data for graphite production reactors, Boiling Water Reactors (BWRs) and Pressurized Water Reactors (PWRs) were added to the dataset and expanded to include a consistent set of data simulated by calculations. A test was performed to determine whether the SFCOMPO/NF dataset will be useful for the analysis and identification of reactor types from isotopic ratios observed in interdicted samples.

  1. Intranuclear interactomic inhibition of NF-κB suppresses LPS-induced severe sepsis

    SciTech Connect (OSTI)

    Park, Sung-Dong; Cheon, So Yeong; Park, Tae-Yoon; Shin, Bo-Young; Oh, Hyunju; Ghosh, Sankar; Koo, Bon-Nyeo; Lee, Sang-Kyou

    2015-08-28

    Suppression of nuclear factor-κB (NF-κB) activation, which is best known as a major regulator of innate and adaptive immune responses, is a potent strategy for the treatment of endotoxic sepsis. To inhibit NF-κB functions, we designed the intra-nuclear transducible form of transcription modulation domain (TMD) of RelA (p65), called nt-p65-TMD, which can be delivered effectively into the nucleus without influencing the cell viability, and work as interactomic inhibitors via disruption of the endogenous p65-mediated transcription complex. nt-p65-TMD effectively inhibited the secretion of pro-inflammatory cytokines, including TNF-α, IL-1β, or IL-6 from BV2 microglia cells stimulated by lipopolysaccharide (LPS). nt-p65-TMD did not inhibit tyrosine phosphorylation of signaling mediators such as ZAP-70, p38, JNK, or ERK involved in T cell activation, but was capable of suppressing the transcriptional activity of NF-κB without the functional effect on that of NFAT upon T-cell receptor (TCR) stimulation. The transduced nt-p65-TMD in T cell did not affect the expression of CD69, however significantly inhibited the secretion of T cell-specific cytokines such as IL-2, IFN-γ, IL-4, IL-17A, or IL-10. Systemic administration of nt-p65-TMD showed a significant therapeutic effect on LPS-induced sepsis model by inhibiting pro-inflammatory cytokines secretion. Therefore, nt-p65-TMD can be a novel therapeutics for the treatment of various inflammatory diseases, including sepsis, where a transcription factor has a key role in pathogenesis, and further allows us to discover new functions of p65 under normal physiological condition without genetic alteration. - Highlights: • The nt-p65-TMD is intra-nuclear interactomic inhibitor of endogenous p65. • The nt-p65-TMD effectively inhibited the secretion of pro-inflammatory cytokines. • The excellent therapeutic potential of nt-p65-TMD was confirmed in sepsis model.

  2. 3D calculation of Tucson-Melbourne 3NF effect in triton binding energy

    SciTech Connect (OSTI)

    Hadizadeh, M. R.; Tomio, L.; Bayegan, S.

    2010-08-04

    As an application of the new realistic three-dimensional (3D) formalism reported recently for three-nucleon (3N) bound states, an attempt is made to study the effect of three-nucleon forces (3NFs) in triton binding energy in a non partial wave (PW) approach. The spin-isospin dependent 3N Faddeev integral equations with the inclusion of 3NFs, which are formulated as function of vector Jacobi momenta, specifically the magnitudes of the momenta and the angle between them, are solved with Bonn-B and Tucson-Melbourne NN and 3N forces in operator forms which can be incorporated in our 3D formalism. The comparison with numerical results in both, novel 3D and standard PW schemes, shows that non PW calculations avoid the very involved angular momentum algebra occurring for the permutations and transformations and it is more efficient and less cumbersome for considering the 3NF.

  3. Interleukin 6 promotes endometrial cancer growth through an autocrine feedback loop involving ERKNF-?B signaling pathway

    SciTech Connect (OSTI)

    Che, Qi; Liu, Bin-Ya; Wang, Fang-Yuan; He, Yin-Yan; Lu, Wen; Liao, Yun; Gu, Wei; Wan, Xiao-Ping

    2014-03-28

    Highlights: IL-6 could promote endometrial cancer cells proliferation. IL-6 promotes its own production through an autocrine feedback loop. ERK and NF-?B pathway inhibitors inhibit IL-6 production and tumor growth. IL-6 secretion relies on the activation of ERKNF-?B pathway axis. An orthotopic nude endometrial carcinoma model confirms the effect of IL-6. - Abstract: Interleukin (IL)-6 as an inflammation factor, has been proved to promote cancer proliferation in several human cancers. However, its role in endometrial cancer has not been studied clearly. Previously, we demonstrated that IL-6 promoted endometrial cancer progression through local estrogen biosynthesis. In this study, we proved that IL-6 could directly stimulate endometrial cancer cells proliferation and an autocrine feedback loop increased its production even after the withdrawal of IL-6 from the medium. Next, we analyzed the mechanism underlying IL-6 production in the feedback loop and found that its production and IL-6-stimulated cell proliferation were effectively blocked by pharmacologic inhibitors of nuclear factor-kappa B (NF-?B) and extra-cellular signal-regulated kinase (ERK). Importantly, activation of ERK was upstream of the NF-?B pathways, revealing the hierarchy of this event. Finally, we used an orthotopic nude endometrial carcinoma model to confirm the effects of IL-6 on the tumor progression. Taken together, these data indicate that IL-6 promotes endometrial carcinoma growth through an expanded autocrine regulatory loop and implicate the ERKNF-?B pathway as a critical mediator of IL-6 production, implying IL-6 to be an important therapeutic target in endometrial carcinoma.

  4. 27-Oxygenated cholesterol induces expression of CXCL8 in macrophages via NF-κB and CD88

    SciTech Connect (OSTI)

    Kim, Sun-Mi; Lee, Chung Won; Kim, Bo-Young; Jung, Young-Suk; Eo, Seong-Kug; Park, Young Chul; Kim, Koanhoi

    2015-08-07

    We attempted to determine the effects of a milieu rich in cholesterol molecules on expression of chemokine CXCL8. A high-cholesterol diet led to an increased transcription of the IL-8 gene in the arteries and elevated levels of CXCL8 in sera of ApoE{sup −/−} mice, compared with those of wild-type C57BL/6 mice. Treatment of THP-1 monocyte/macrophage cells with 27-hydroxycholesterol (27OHChol) resulted in transcription of the IL-8 gene and increased secretion of its corresponding gene product whereas cholesterol did not induce expression of CXCL8 in THP-1 cells. 27OHChol-induced transcription of the IL-8 gene was blocked by cycloheximide, but not by polymyxin B. Treatment of THP-1 cells with 27OHChol caused translocation of p65 NF-κB subunit into the nucleus and up-regulation of CD88. Inhibition of NF-κB and CD88 using SN50 and W-54011, respectively, resulted in reduced transcription of the IL-8 gene and attenuated secretion of CXCL8 induced by 27OHChol. We propose that oxidatively modified cholesterol like 27OHChol, rather than cholesterol, is responsible for sustained expression of CXCL8 in monocytes/macrophages in atherosclerotic arteries. - Highlights: • Consumption of a high-cholesterol diet leads to increased CXCL8 expression in ApoE{sup −/−} mice. • 27OHChol, but not cholesterol, up-regulates expression of CXCL8 in macrophages. • 27OHChol enhances nuclear translocation of NF-κB and expression of CD88 in macrophages. • Inhibition of NF-κB or CD88 results in decreased CXCL8 expression induced by 27OHChol. • 27OHChol up-regulates CXCL8 expression via NF-κB and CD88 in macrophages.

  5. miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

    SciTech Connect (OSTI)

    Zhang, Yu; Wei, Guangkuan; Di, Zhiyong; Zhao, Qingjie

    2014-09-26

    Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.

  6. PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

    SciTech Connect (OSTI)

    Zheng, Peihao; Guo, Honggang; Li, Guangchao; Han, Siqi; Luo, Fei; Liu, Yi

    2015-03-06

    Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expression of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.

  7. High glucose induces activation of NF-κB inflammatory signaling through IκBα sumoylation in rat mesangial cells

    SciTech Connect (OSTI)

    Huang, Wei; Department of Endocrinology, The People’s Hospital of Xindu, Xindu, Sichuang, 610500 ; Xu, Ling; Zhou, Xueqin; Department of Endocrinology, The People’s Hospital of Leshan, Sichuang ; Gao, Chenlin; Yang, Maojun; Chen, Guo; Zhu, Jianhua; Jiang, Lan; Gan, Huakui; Gou, Fang; Feng, Hong; Peng, Juan; Xu, Yong

    2013-08-30

    Highlights: •The expression of SUMO1, SUMO2/3 under high glucose was obviously enhanced. •High glucose induced degradation of IκBα and activation of NF-κB pathway. •Sumoylation of IκBα in high glucose were significantly decreased. •The proteasome inhibitor MG132 could partially revert the degradation of IκBα. -- Abstract: The posttranslational modification of proteins by small ubiquitin-like modifiers (SUMOs) has emerged as an important regulatory mechanism for the alteration of protein activity, stability, and cellular localization. The latest research demonstrates that sumoylation is extensively involved in the regulation of the nuclear factor κB (NF-κB) pathway, which plays a critical role in the regulation of inflammation and contributes to fibrosis in diabetic nephropathy (DN). However, the role of sumoylation in the regulation of NF-κB signaling in DN is still unclear. In the present study, we cultured rat glomerular mesangial cells (GMCs) stimulated by high glucose and divided GMCs into six groups: normal glucose group (5.6 mmol/L), high glucose groups (10, 20, and 30 mmol/L), mannitol group (i.e., osmotic control group), and MG132 intervention group (30 mmol/L glucose with MG132, a proteasome inhibitor). The expression of SUMO1, SUMO2/3, IκBα, NF-κBp65, and monocyte chemotactic protein 1 (MCP-1) was measured by Western blot, reverse-transcription polymerase chain reaction, and indirect immunofluorescence laser scanning confocal microscopy. The interaction between SUMO1, SUMO2/3, and IκBα was observed by co-immunoprecipitation. The results showed that the expression of SUMO1 and SUMO2/3 was dose- and time-dependently enhanced by high glucose (p < 0.05). However, the expression of IκBα sumoylation in high glucose was significantly decreased compared with the normal glucose group (p < 0.05). The expression of IκBα was dose- and time-dependently decreased, and NF-κBp65 and MCP-1 were increased under high glucose conditions, which

  8. Thermal Reactions of Uranium Metal, UO2, U3O8, UF4, and UO2F2 with NF3 to Produce UF6

    SciTech Connect (OSTI)

    McNamara, Bruce K.; Scheele, Randall D.; Kozelisky, Anne E.; Edwards, Matthew K.

    2009-11-01

    he objective of this paper is to demonstrate that NF3 fluorinates uranium metal, UO2, UF4, UO3, U3O8, and UO2F22H2O to produce the volatile UF6 at temperatures between 100 and 500?C. Thermogravimetric reaction profiles are described that reflect changes in the uranium oxidation state and discrete chemical speciation. Differences in the onset temperatures for each system indicate that NF3-substrate interactions are important for the temperature at which NF3 reacts: U metal > UO3 > UO2 > UO2F2 > UF4 and in fact may indicate different fluorination mechanisms for these various substrates. These studies demonstrate that NF3 is a potential replacement fluorinating agent in the existing nuclear fuel cycle and in oft-proposed actinide volatility reprocessing.

  9. Microstructural evolution of NF709 (20Cr–25Ni–1.5MoNbTiN) under neutron irradiation

    DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

    Kim, Byoungkoo; Tan, Lizhen; Xu, C.; Yang, Yong; Zhang, Xuan; Li, Meimei

    2015-12-30

    In this study, because of its superior creep and corrosion resistance as compared with general austenitic stainless steels, NF709 has emerged as a candidate structural material for advanced nuclear reactors. To obtain fundamental information about the radiation resistance of this material, this study examined the microstructural evolution of NF709 subjected to neutron irradiation to 3 displacements per atom at 500 °C. Transmission electron microscopy, scanning electron microscopy, and high-energy x-ray diffraction were employed to characterize radiation-induced segregation, Frank loops, voids, as well as the formation and reduction of precipitates. Radiation hardening of ~76% was estimated by nanoindentation, approximately consistent withmore » the calculation according to the dispersed barrier-hardening model, suggesting Frank loops as the primary hardening source.« less

  10. Microstructural evolution of NF709 (20Cr–25Ni–1.5MoNbTiN) under neutron irradiation

    SciTech Connect (OSTI)

    Kim, Byoungkoo; Tan, Lizhen; Xu, C.; Yang, Yong; Zhang, Xuan; Li, Meimei

    2015-12-30

    In this study, because of its superior creep and corrosion resistance as compared with general austenitic stainless steels, NF709 has emerged as a candidate structural material for advanced nuclear reactors. To obtain fundamental information about the radiation resistance of this material, this study examined the microstructural evolution of NF709 subjected to neutron irradiation to 3 displacements per atom at 500 °C. Transmission electron microscopy, scanning electron microscopy, and high-energy x-ray diffraction were employed to characterize radiation-induced segregation, Frank loops, voids, as well as the formation and reduction of precipitates. Radiation hardening of ~76% was estimated by nanoindentation, approximately consistent with the calculation according to the dispersed barrier-hardening model, suggesting Frank loops as the primary hardening source.

  11. Deactivation Management

    Broader source: Energy.gov [DOE]

    The purpose here is to provide information for specific aspects of project management that apply to deactivation. Overall management of deactivation projects should use a traditional project...

  12. Microsoft PowerPoint - Managing Uncertainty and Demonstrating Compliance

    Office of Environmental Management (EM)

    Low Dose DOE's Low Dose R di ti R h R di ti R h Radiation Research Radiation Research Program Program g g NF Metting, Sc.D., Program Manager Nuclear Energy Advisory Committee Meeting Nuclear Energy Advisory Committee Meeting L'Enfant Plaza Hotel L'Enfant Plaza Hotel 6 December 2012 Office of Science Office of Biological and Environmental Research DOE's Low Dose Program: DOE s Low Dose Program: Is unique within the U.S. government in focusing on low dose biological research aimed at informing

  13. Subneurotoxic copper(II)-induced NF-κB-dependent microglial activation is associated with mitochondrial ROS

    SciTech Connect (OSTI)

    Hu, Zhuqin; Yu, Fengxiang; Gong, Ping; Qiu, Yu; Zhou, Wei; Cui, Yongyao; Li, Juan Chen, Hongzhuan

    2014-04-15

    Microglia-mediated neuroinflammation and the associated neuronal damage play critical roles in the pathogenesis of neurodegenerative disorders. Evidence shows an elevated concentration of extracellular copper(II) in the brains of these disorders, which may contribute to neuronal death through direct neurotoxicity. Here we explored whether extracellular copper(II) triggers microglial activation. Primary rat microglia and murine microglial cell line BV-2 cells were cultured and treated with copper(II). The content of tumor necrosis factor-α (TNF-α) and nitric oxide in the medium was determined. Extracellular hydrogen peroxide was quantified by a fluorometric assay with Amplex Red. Mitochondrial superoxide was measured by MitoSOX oxidation. At subneurotoxic concentrations, copper(II) treatment induced a dose- and time-dependent release of TNF-α and nitric oxide from microglial cells, and caused an indirect, microglia-mediated neurotoxicity that was blocked by inhibition of TNF-α and nitric oxide production. Copper(II)-initiated microglial activation was accompanied with reduced IkB-α expression as well as phosphorylation and translocation of nuclear factor-κB (NF-κB) p65 and was blocked by NF-κB inhibitors (BAY11-7082 and SC-514). Moreover, copper(II) treatment evoked a rapid release of hydrogen peroxide from microglial cells, an effect that was not affected by NADPH oxidase inhibitors. N-acetyl-cysteine, a scavenger of reactive oxygen species (ROS), abrogated copper(II)-elicited microglial release of TNF-α and nitric oxide and subsequent neurotoxicity. Importantly, mitochondrial production of superoxide, paralleled to extracellular release of hydrogen peroxide, was induced after copper(II) stimulation. Our findings suggest that extracellular copper(II) at subneurotoxic concentrations could trigger NF-κB-dependent microglial activation and subsequent neurotoxicity. NADPH oxidase-independent, mitochondria-derived ROS may be involved in this activation

  14. Conference Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1999-11-03

    To establish requirements and responsibilities with respect to managing conferences sponsored by the Department of Energy (DOE) or by DOE management and operating contractors and other contractors who perform work at DOE-owned or -leased facilities, including management and integration contractors and environmental restoration management contractors (when using funds that will be reimbursed by DOE). Cancels DOE N 110.3.

  15. SENP1 inhibition induces apoptosis and growth arrest of multiple myeloma cells through modulation of NF-κB signaling

    SciTech Connect (OSTI)

    Xu, Jun; Sun, Hui-Yan; Xiao, Feng-Jun; Wang, Hua; Yang, Yang; Wang, Lu; Gao, Chun-Ji; Guo, Zi-Kuan; Wu, Chu-Tse; Wang, Li-Sheng

    2015-05-01

    SUMO/sentrin specific protease 1 (Senp1) is an important regulation protease in the protein sumoylation, which affects the cell cycle, proliferation and differentiation. The role of Senp1 mediated protein desumoylation in pathophysiological progression of multiple myeloma is unknown. In this study, we demonstrated that Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. Lentivirus-mediated Senp1 knockdown triggers apoptosis and reduces viability, proliferation and colony forming ability of MM cells. The NF-κB family members including P65 and inhibitor protein IkBα play important roles in regulation of MM cell survival and proliferation. We further demonstrated that Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation, leading to inactivation of NF-kB signaling in MM cells. These results delineate a key role for Senp1in IL-6 induced proliferation and survival of MM cells, suggesting it may be a potential new therapeutic target in MM. - Highlights: • Senp1 is overexpressed and induced by IL-6 in multiple myeloma cells. • Senp1 knockdown triggers apoptosis and reduces proliferation of MM cells. • Senp1 inhibition decreased IL-6-induced P65 and IkBα phosphorylation.

  16. Oxaliplatin antagonizes HIV-1 latency by activating NF-κB without causing global T cell activation

    SciTech Connect (OSTI)

    Zhu, Xiaoli; Liu, Sijie; Wang, Pengfei; Qu, Xiying; Wang, Xiaohui; Zeng, Hanxian; Chen, Huabiao; Zhu, Huanzhang

    2014-07-18

    Highlights: • The chemotherapeutic drug oxaliplatin reactivates latent HIV-1 in this cell line model of HIV-1 latency. • Reactivation is synergized when oxaliplatin is used in combination with valproic acid. • Oxaliplatin reactivates latent HIV-1 through activation of NF-kB and does not induce T cell activation. - Abstract: Reactivation of latent HIV-1 is a promising strategy for the clearance of the viral reservoirs. Because of the limitations of current agents, identification of new latency activators is urgently required. Using an established model of HIV-1 latency, we examined the effect of Oxaliplatin on latent HIV-1 reactivation. We showed that Oxaliplatin, alone or in combination with valproic acid (VPA), was able to reactivate HIV-1 without inducing global T cell activation. We also provided evidence that Oxaliplatin reactivated HIV-1 expression by inducing nuclear factor kappa B (NF-κB) nuclear translocation. Our results indicated that Oxaliplatin could be a potential drug candidate for anti-latency therapies.

  17. Data Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Data Management Data Management PDSF and IHEP, in Beijing, China, are the two main computing facitilies for the Daya Bay experiment with PDSF being used primarily by North American ...

  18. MANAGEMENT ALERT

    Broader source: Energy.gov (indexed) [DOE]

    COMMISSION FROM: Gregory H. Friedman Inspector General SUBJECT: INFORMATION: Management Alert: Review of Allegations of Improper Disclosure of Confidential, Nonpublic...

  19. Urotensin II increases foam cell formation by repressing ABCA1 expression through the ERK/NF-κB pathway in THP-1 macrophages

    SciTech Connect (OSTI)

    Wang, Yan; Wu, Jian-Feng; Tang, Yan-Yan; Zhang, Min; Li, Yuan; Chen, Kong; Zeng, Meng-Ya; Yao, Feng; Xie, Wei; Zheng, Xi-Long; Zeng, Gao-Feng; Tang, Chao-Ke

    2014-10-03

    Highlights: • U II reduces cholesterol efflux in THP-1 macrophages. • U II decreases the expression of ABCA1. • Inhibition of the ERK/NF-κB pathway reduces U II effects on ABCA1 expression and cholesterol efflux. - Abstract: Objective: Foam cell formation in the arterial wall plays a key role in the development of atherosclerosis. Recent studies showed that Urotensin II (U II) is involved in the pathogenesis of atherosclerosis. Here we examined the effects of human U II on ATP-binding cassette transporter A1 (ABCA1) expression and the underlying mechanism in THP-1 macrophages. Methods and results: Cultured THP-1 macrophages were treated with U II, followed by measuring the intracellular lipid contents, cholesterol efflux and ABCA1 levels. The results showed that U II dramatically decreased ABCA1 levels and impaired cholesterol efflux. However, the effects of U II on ABCA1 protein expression and cellular cholesterol efflux were partially reversed by inhibition of extracellular signal regulated kinase 1/2 (ERK1/2) and nuclear factor kappa B (NF-κB) activity, suggesting the potential roles of ERK1/2 and NF-κB in ABCA1 expression, respectively. Conclusion: Our current data indicate that U II may have promoting effects on the progression of atherosclerosis, likely through suppressing ABCA1 expression via activation of the ERK/NF-κB pathway and reducing cholesterol efflux to promote macrophage foam cell formation.

  20. Integrated Genomic Analysis Identifies Clinically Relevant Subtypes of Glioblastoma Characterized by Abnormalities in PDGFRA, IDH1, EGFR, and NF1

    SciTech Connect (OSTI)

    Verhaak, Roel GW; Hoadley, Katherine A; Purdom, Elizabeth; Wang, Victoria; Qi, Yuan; Wilkerson, Matthew D; Miller, C Ryan; Ding, Li; Golub, Todd; Mesirov, Jill P; Alexe, Gabriele; Lawrence, Michael; O'Kelly, Michael; Tamayo, Pablo; Weir, Barbara A; Gabriel, Stacey; Winckler, Wendy; Gupta, Supriya; Jakkula, Lakshmi; Feiler, Heidi S; Hodgson, J Graeme; James, C David; Sarkaria, Jann N; Brennan, Cameron; Kahn, Ari; Spellman, Paul T; Wilson, Richard K; Speed, Terence P; Gray, Joe W; Meyerson, Matthew; Getz, Gad; Perou, Charles M; Hayes, D Neil; Network, The Cancer Genome Atlas Research

    2009-09-03

    The Cancer Genome Atlas Network recently cataloged recurrent genomic abnormalities in glioblastoma multiforme (GBM). We describe a robust gene expression-based molecular classification of GBM into Proneural, Neural, Classical, and Mesenchymal subtypes and integrate multidimensional genomic data to establish patterns of somatic mutations and DNA copy number. Aberrations and gene expression of EGFR, NF1, and PDGFRA/IDH1 each define the Classical, Mesenchymal, and Proneural subtypes, respectively. Gene signatures of normal brain cell types show a strong relationship between subtypes and different neural lineages. Additionally, response to aggressive therapy differs by subtype, with the greatest benefit in the Classical subtype and no benefit in the Proneural subtype. We provide a framework that unifies transcriptomic and genomic dimensions for GBM molecular stratification with important implications for future studies.

  1. Metastatic function of BMP-2 in gastric cancer cells: The role of PI3K/AKT, MAPK, the NF-{kappa}B pathway, and MMP-9 expression

    SciTech Connect (OSTI)

    Kang, Myoung Hee; Oh, Sang Cheul; Kang, Han Na; Kim, Jung Lim; Kim, Jun Suk

    2011-07-15

    Bone morphogenetic proteins (BMPs) have been implicated in tumorigenesis and metastatic progression in various types of cancer cells, but the role and cellular mechanism in the invasive phenotype of gastric cancer cells is not known. Herein, we determined the roles of phosphoinositide 3-kinase (PI3K)/AKT, extracellular signal-regulated protein kinase (ERK), nuclear factor (NF)-{kappa}B, and matrix metalloproteinase (MMP) expression in BMP-2-mediated metastatic function in gastric cancer. We found that stimulation of BMP-2 in gastric cancer cells enhanced the phosphorylation of AKT and ERK. Accompanying activation of AKT and ERK kinase, BMP-2 also enhanced phosphorylation/degradation of I{kappa}B{alpha} and the nuclear translocation/activation of NF-{kappa}B. Interestingly, blockade of PI3K/AKT and ERK signaling using LY294002 and PD98059, respectively, significantly inhibited BMP-2-induced motility and invasiveness in association with the activation of NF-{kappa}B. Furthermore, BMP-2-induced MMP-9 expression and enzymatic activity was also significantly blocked by treatment with PI3K/AKT, ERK, or NF-{kappa}B inhibitors. Immunohistochemistry staining of 178 gastric tumor biopsies indicated that expression of BMP-2 and MMP-9 had a significant positive correlation with lymph node metastasis and a poor prognosis. These results indicate that the BMP-2 signaling pathway enhances tumor metastasis in gastric cancer by sequential activation of the PI3K/AKT or MAPK pathway followed by the induction of NF-{kappa}B and MMP-9 activity, indicating that BMP-2 has the potential to be a therapeutic molecular target to decrease metastasis.

  2. Nuclear NF-?B Expression Correlates With Outcome Among Patients With Head and Neck Squamous Cell Carcinoma Treated With Primary Chemoradiation Therapy

    SciTech Connect (OSTI)

    Balermpas, Panagiotis [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Michel, Yvonne [Senckenberg Institute of Pathology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Senckenberg Institute of Pathology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Wagenblast, Jens [Department of Otorhinolaryngology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Otorhinolaryngology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Seitz, Oliver [Department of Maxillofacial Surgery, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Maxillofacial Surgery, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Sipek, Florian; Rdel, Franz; Rdel, Claus [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany); Fokas, Emmanouil, E-mail: emmanouil.fokas@kgu.de [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)] [Department of Radiation Therapy and Oncology, J. W. Goethe University Frankfurt am Main, Frankfurt (Germany)

    2013-07-15

    Background: To examine whether nuclear NF-?B expression correlates with outcome in patients with head and neck squamous cell carcinoma (HNSCC) treated with primary chemoradiation therapy (CRT). Methods and Materials: Between 2007 and 2010, 101 patients with locally advanced primary HNSCC were treated with definitive simultaneous CRT. Pretreatment biopsy specimens were analyzed for NF-?B p65 (RelA) nuclear immunoreactivity. A sample was assigned to be positive with more than 5% positive nuclear expression. The predictive relevance of NF-?B and clinicopathologic factors for overall survival (OS), progression-free survival (PFS), local progression-free survival (LPFS), and metastasis-free survival (DMFS) was examined by univariate and multivariate analysis. Results: No significant differences between the groups were observed with regard to age, sex, total radiation dose, fractionation mode, total chemotherapy applied, T stage or grading. Patients with p65 nuclear positive biopsy specimens showed significantly a higher rate of lymph node metastasis (cN2c or cN3 status, P=.034). Within a mean follow-up time of 25 months (range, 2.33-62.96 months) OS, PFS, and DMFS were significantly poorer in the p65 nuclear positive group (P=.008, P=.027, and P=.008, respectively). These correlations remained significant in multivariate analysis. Conclusion: NF-?B/p65 nuclear expression is associated with increased lymphatic and hematogenous tumor dissemination and decreased survival in HNSCC patients treated with primary CRT. Our results may foster further investigation of a predictive relevance of NF-?B/p65 and its role as a suitable target for a molecular-based targeted therapy in HNSCC cancer.

  3. Environmental Management Waste Management Facility (EMWMF) at...

    Office of Environmental Management (EM)

    Review of the Environmental Management Waste Management Facility (EMWMF) at Oak Ridge ... INTRODUCTION The Environmental Management Waste Management Facility (EMWMF) is a land ...

  4. Teaching Managers How to Manage

    SciTech Connect (OSTI)

    Hylko, J.M.

    2006-07-01

    Following graduation from a college or university with a technical degree, or through years of experience, an individual's training and career development activities typically focus on enhancing technical problem-solving skills. However, as these technical professionals, herein referred to as 'Techies', advance throughout their careers, they may be required to accept and adapt to the role of being a manager, and must undergo a transition to learn and rely on new problem-solving skills. However, unless a company has a specific manager-trainee class to address this subject and develop talent from within, an employee's management style is learned and developed 'on the job'. Both positive and negative styles are nurtured by those managers having similar qualities. Unfortunately, a negative style often contributes to the deterioration of employee morale and ultimate closing of a department or company. This paper provides the core elements of an effective management training program for 'Teaching Managers How to Manage' derived from the Department of Energy's Integrated Safety Management System and the Occupational Safety and Health Administration 's Voluntary Protection Program. Discussion topics and real-life examples concentrate on transitioning an employee from a 'Techie' to a manager; common characteristics of being a manager; the history and academic study of management; competition, change and the business of waste management; what to do after taking over a department by applying Hylko's Star of Success; command media; the formal and informal organizational charts; chain of command; hiring and developing high-degree, autonomous employees through effective communication and delegation; periodic status checks; and determining if the program is working successfully. These common characteristics of a strong management/leadership culture and practical career tips discussed herein provide a solid foundation for any company or department that is serious about developing

  5. Quality Management

    Broader source: Energy.gov [DOE]

    The Office of Quality Management, within the Office of Health, Safety and Security develops policies and procedures to ensure the classification and control of information is effective and...

  6. Energy Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Energy Management Utilize energy efficiency to improve your industrial customer's business performance without the cost of major capital improvements. Energy efficiency is not...

  7. Deputy Manager

    Broader source: Energy.gov [DOE]

    A successful candidate in this position will support the Oak Ridge Office (ORO) Manager by providing overall executive leadership to ORO.

  8. Position Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1992-06-23

    The order prescribes the policies, responsibilities, and procedures for position management within (DOE). Canceled by DOE N 1321.140. Cancels DOE 3510.1

  9. project management

    National Nuclear Security Administration (NNSA)

    3%2A en Project Management and Systems Support http:www.nnsa.energy.govaboutusouroperationsapmprojectmanagementandsystemssupport

  10. project management

    National Nuclear Security Administration (NNSA)

    %2A en Project Management and Systems Support http:nnsa.energy.govaboutusouroperationsapmprojectmanagementandsystemssupport

  11. Bibliographic Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    and more from varied resources and databases to organize references, keep notes to stay ahead of the game. Questions? 505-667-5809 Email Bibliographic management tools allow...

  12. acquisition management

    National Nuclear Security Administration (NNSA)

    the science, technology, and engineering base; and,

  13. Continue NNSA management reforms.


    • Our Values:<...

    • Coactivation of the PI3K/Akt and ERK signaling pathways in PCB153-induced NF-κB activation and caspase inhibition

      SciTech Connect (OSTI)

      Liu, Changjiang; Yang, Jixin; Fu, Wenjuan; Qi, Suqin; Wang, Chenmin; Quan, Chao; Yang, Kedi

      2014-06-15

      Polychlorinated biphenyls (PCBs) are a group of persistent and widely distributed environmental pollutants that have various deleterious effects, e.g., neurotoxicity, endocrine disruption and reproductive abnormalities. In order to verify the hypothesis that the PI3K/Akt and MAPK pathways play important roles in hepatotoxicity induced by PCBs, Sprague–Dawley (SD) rats were dosed with PCB153 intraperitoneally at 0, 4, 16 and 32 mg/kg for five consecutive days; BRL cells (rat liver cell line) were treated with PCB153 (0, 1, 5, and 10 μM) for 24 h. Results indicated that the PI3K/Akt and ERK pathways were activated in vivo and in vitro after exposure to PCB153, and protein levels of phospho-Akt and phospho-ERK were significantly increased. Nuclear factor-κB (NF-κB) activation and caspase-3, -8 and -9 inhibition caused by PCB153 were also observed. Inhibiting the ERK pathway significantly attenuated PCB153-induced NF-κB activation, whereas inhibiting the PI3K/Akt pathway hardly influenced phospho-NF-κB level. However, inhibiting the PI3K/Akt pathway significantly elevated caspase-3, -8 and -9 activities, while the ERK pathway only synergistically regulated caspase-9. Proliferating cell nuclear antigen (PCNA), a reliable indicator of cell proliferation, was also induced. Moreover, PCB153 led to hepatocellular hypertrophy and elevated liver weight. Taken together, PCB153 leads to aberrant proliferation and apoptosis of hepatocytes through NF-κB activation and caspase inhibition, and coactivated PI3K/Akt and ERK pathways play critical roles in PCB153-induced hepatotoxicity. - Highlights: • PCB153 led to hepatotoxicity through NF-κB activation and caspase inhibition. • The PI3K/Akt and ERK pathways were coactivated in vivo and in vitro by PCB153. • The ERK pathway regulated levels of phospho-NF-κB and caspase-9. • The PI3K/Akt pathway regulated levels of caspase-3, -8 and -9.

    • Gastrodin stimulates anticancer immune response and represses transplanted H22 hepatic ascitic tumor cell growth: Involvement of NF-?B signaling activation in CD4 + T cells

      SciTech Connect (OSTI)

      Shu, Guangwen; Yang, Tianming; Wang, Chaoyuan; Su, Hanwen; Xiang, Meixian

      2013-06-15

      Gastrodia elata Blume (G. elata) is a famous restorative food in East Asia. It can be used as an auxiliary reagent in hepatocellular carcinoma (HCC) treatment. Previous studies unveiled that G. elata exhibited immunomodulatory activities. To explore the active ingredients contributing to its immunomodulatory activities, gastrodin, vanillin, and parishin B were purified from G. elata and their anti-HCC effects were assessed in vivo. Among these compounds, only gastrodin was capable of repressing transplanted H22 ascitic hepatic tumor cell growth in vivo with low toxicity. Further investigations were designed to explore the effects of gastrodin on the immune system of tumor-bearing mice and potential molecular mechanisms underlying these effects. Our data showed that gastrodin ameliorated tumor cell transplantation-induced activation of endogenous pro-apoptotic pathway in CD4 + T cells and abnormalities in serum cytokine profiles in host animals. These events enhanced cytotoxic activities of natural killer and CD8 + T cells against H22 hepatic cancer cells. Gastrodin administration specifically upregulated mRNA levels of several nuclear factor ?B (NF-?B) responsive genes in CD4 + T cells but not in CD8 + T cells. Chromatin immunoprecipitation assay showed that gastrodin increased the association of NF-?B p65 subunit to the promoter regions of IL-2 and Bcl-2 encoding genes in CD4 + T cells. Our investigations demonstrated that gastrodin is the main active ingredient contributing to the anticancer immunomodulatory properties of G. elata. Promoting NF-?B-mediated gene transcription in CD4 + T cells is implicated in its immunomodulatory activity. - Highlights: Gastrodin stimulates anticancer immune response. Gastrodin represses tumor transplantation-induced CD4 + T cell apoptosis. Gastrodin activates NF-?B activity in CD4 + T cells.

    • Moderate extracellular acidification inhibits capsaicin-induced cell death through regulating calcium mobilization, NF-{kappa}B translocation and ROS production in synoviocytes

      SciTech Connect (OSTI)

      Hu, Fen; Yang, Shuang; Zhao, Dan; Zhu, Shuyan; Wang, Yuxiang; Li, Junying

      2012-07-20

      Highlights: Black-Right-Pointing-Pointer Moderate extracellular acidification regulates intracellular Ca{sup 2+} mobilization. Black-Right-Pointing-Pointer Moderate acidification activates NF-{kappa}B nuclear translocation in synoviocytes. Black-Right-Pointing-Pointer Moderate acidification depresses the ROS production induced by capsaicin. Black-Right-Pointing-Pointer Moderate acidification inhibits capsaicin-caused synoviocyte death. -- Abstract: We previously show the expression of transient receptor potential vanilloid 1 (TRPV1) in primary synoviocytes from collagen-induced arthritis (CIA) rats. Capsaicin and lowered extracellular pH from 7.4 to 5.5 induce cell death through TRPV1-mediated Ca{sup 2+} entry and reactive oxygen species (ROS) production. However, under the pathological condition in rheumatoid arthritis, the synovial fluid is acidified to a moderate level (about pH 6.8). In the present study, we examined the effects of pH 6.8 on the TRPV1-mediated cell death. Our finding is different or even opposite from what was observed at pH 5.5. We found that the moderate extracellular acidification (from pH 7.4 to 6.8) inhibited the capsaicin-induced Ca{sup 2+} entry through attenuating the activity of TRPV1. In the mean time, it triggered a phospholipse C (PLC)-related Ca{sup 2+} release from intracellular stores. The nuclear translocation of NF-{kappa}B was found at pH 6.8, and this also depends on PLC activation. Moreover, the capsaicin-evoked massive ROS production and cell death were depressed at pH 6.8, both of which are dependent on the activation of PLC and NF-{kappa}B. Taken together, these results suggested that the moderate extracellular acidification inhibited the capsaicin-induced synoviocyte death through regulating Ca{sup 2+} mobilization, activating NF-{kappa}B nuclear translocation and depressing ROS production.

    • Stevioside ameliorates high-fat diet-induced insulin resistance and adipose tissue inflammation by downregulating the NF-{kappa}B pathway

      SciTech Connect (OSTI)

      Wang, Zhiquan; Xue, Liqiong; Guo, Cuicui; Han, Bing; Pan, Chunming; Zhao, Shuangxia; Song, Huaidong; Ma, Qinyun

      2012-01-27

      Highlights: Black-Right-Pointing-Pointer Stevioside ameliorates high-fat diet-induced insulin resistance. Black-Right-Pointing-Pointer Stevioside alleviates the adipose tissue inflammation. Black-Right-Pointing-Pointer Stevioside reduces macrophages infiltration into the adipose tissue. Black-Right-Pointing-Pointer Stevioside suppresses the activation of NF-{kappa}B in the adipose tissue. -- Abstract: Accumulating evidence suggests that adipose tissue is the main source of pro-inflammatory molecules that predispose individuals to insulin resistance. Stevioside (SVS) is a widely used sweetener with multiple beneficial effects for diabetic patients. In this study, we investigated the effect of SVS on insulin resistance and the pro-inflammatory state of adipose tissue in mice fed with a high-fat diet (HFD). Oral administration of SVS for 1 month had no effect on body weight, but it significantly improved fasting glucose, basal insulin levels, glucose tolerance and whole body insulin sensitivity. Interestingly, these changes were accompanied with decreased expression levels of several inflammatory cytokines in adipose tissue, including TNF-{alpha}, IL6, IL10, IL1{beta}, KC, MIP-1{alpha}, CD11b and CD14. Moreover, macrophage infiltration in adipose tissue was remarkably reduced by SVS. Finally, SVS significantly suppressed the nuclear factor-kappa b (NF-{kappa}B) signaling pathway in adipose tissue. Collectively, these results suggested that SVS may ameliorate insulin resistance in HFD-fed mice by attenuating adipose tissue inflammation and inhibiting the NF-{kappa}B pathway.

    • ROS and NF-{kappa}B are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes

      SciTech Connect (OSTI)

      Ye Shefang Wu Yihui; Hou Zhenqing; Zhang Qiqing

      2009-02-06

      Carbon nanotubes (CNTs) have potential applications in biosensors, tissue engineering, and biomedical devices because of their unique physico-chemical, electronic and mechanical properties. However, there is limited literature data available concerning the biological properties and toxicity of CNTs. This study aimed to assess the toxicity exhibited by multi-walled CNTs (MWCNTs) and to elucidate possible molecular mechanisms underlying the biological effects of MWCNTs in A549 cells. Exposing A549 cells to MWCNTs led to cell death, changes in cell size and complexity, reactive oxygen species (ROS) production, interleukin-8 (IL-8) gene expression and nuclear factor (NF)-{kappa}B activation. Treatment of A549 cells with antioxidants prior to adding MWCNTs decreased ROS production and abrogated expression of IL-8 mRNA. Pretreatment of A549 cells with NF-{kappa}B inhibitors suppressed MWCNTs-induced IL-8 mRNA expression. These results indicate that MWCNTs are able to induce expression of IL-8 in A549 cells, at least in part, mediated by oxidative stress and NF-{kappa}B activation.

    • Neutron and proton electric dipole moments from Nf=2+1 domain-wall fermion lattice QCD

      DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)

      Shintani, Eigo; Blum, Thomas; Izubuchi, Taku; Soni, Amarjit

      2016-05-05

      We present a lattice calculation of the neutron and proton electric dipole moments (EDM’s) with Nf = 2 + 1 flavors of domain-wall fermions. The neutron and proton EDM form factors are extracted from three-point functions at the next-to-leading order in the θ vacuum of QCD. In this computation, we use pion masses 330 and 420 MeV and 2.7 fm3 lattices with Iwasaki gauge action and a 170 MeV pion and 4.6 fm3 lattice with I-DSDR gauge action, all generated by the RBC and UKQCD collaborations. The all-mode-averaging technique enables an efficient, high statistics calculation; however the statistical errors onmore » our results are still relatively large, so we investigate a new direction to reduce them, reweighting with the local topological charge density which appears promising. Furthermore, we discuss the chiral behavior and finite size effects of the EDM’s in the context of baryon chiral perturbation theory.« less

    • Conference Management

      Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

      2007-01-25

      The Order establishes requirements and responsibilities for managing conferences sponsored or co-sponsored by the Department of Energy, including the National Nuclear Security Administration. Cancels DOE O 110.3. Canceled by DOE N 251.97.

    • PROJECT MANAGEMENT PLANS Project Management Plans

      Office of Environmental Management (EM)

      MANAGEMENT PLANS Project Management Plans Overview Project Management Plan Suggested Outline Subjects Crosswalk between the Suggested PMP Outline Subjects and a Listing ...

  1. Summary - Environmental Management Waste Management Facility...

    Office of Environmental Management (EM)

    Waste Management Facility (EMWMF) at Oak Ridge, TN Why DOE-EM Did This Review The Environmental Management Waste Management Facility (EMWMF) is a land disposal facility for ...

  2. Environmental Management Waste Management Facility (EMWMF) at...

    Office of Environmental Management (EM)

    Waste Management Facility (EMWMF) at Oak Ridge Environmental Management Waste Management Facility (EMWMF) at Oak Ridge Full Document and Summary Versions are available for download ...

  3. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Introduction to the DOE-Managed Spent Nuclear Fuel and High Level Waste Research (aka Defense Repository) David Sevougian, Kevin McMahon Sandia National Laboratories Used Fuel Disposition Working Group Meeting Las Vegas, Nevada June 7-9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under

  4. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Managed Spent Nuclear Fuel (SNF) and High Level Waste (HLW) Repository - Inventory Overview David C. Sassani Sandia National Laboratories Used Fuel Disposition Campaign Annual Working Group Meeting June 6-9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5401

  5. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    DOE-Managed SNF and HLW Research: Preliminary Design Concepts -Work Package Overview -Waste Package Considerations -DREP Salt Design Concept Ed Matteo, Ernie Hardin, Mark Rigali, Teklu Hadgu, Heeho Park UFD Working Group University of Nevada/Las Vegas June 7-9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security

  6. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Debrief for the DOE-Managed Spent Nuclear Fuel and High Level Waste Research (aka Defense Repository) David Sevougian, Kevin McMahon Sandia National Laboratories Used Fuel Disposition Working Group Meeting Las Vegas, Nevada June 7-9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract

  7. Ascorbic acid suppresses endotoxemia and NF-κB signaling cascade in alcoholic liver fibrosis in guinea pigs: A mechanistic approach

    SciTech Connect (OSTI)

    Abhilash, P.A.; Harikrishnan, R.; Indira, M.

    2014-01-15

    Alcohol consumption increases the small intestinal bacterial overgrowth (SIBO) and intestinal permeability of endotoxin. The endotoxin mediated inflammatory signaling plays a major role in alcoholic liver fibrosis. We evaluated the effect of ascorbic acid (AA), silymarin and alcohol abstention on the alcohol induced endotoxemia and NF-κB activation cascade pathway in guinea pigs (Cavia porcellus). Guinea pigs were administered ethanol at a daily dose of 4 g/kg b.wt for 90 days. After 90 days, ethanol administration was stopped. The ethanol treated animals were divided into abstention, silymarin (250 mg/kg b.wt) and AA (250 mg/kg b.wt) supplemented groups and maintained for 30 days. The SIBO, intestinal permeability and endotoxin were significantly increased in the ethanol group. The mRNA expressions of intestinal proteins claudin, occludin and zona occludens-1 were significantly decreased in ethanol group. The mRNA levels of inflammatory receptors, activity of IKKβ and the protein expressions of phospho-IκBα, NF-κB, TNF-α, TGF-β{sub 1} and IL-6 were also altered in ethanol group. The expressions of fibrosis markers α-SMA, α{sub 1} (I) collagen and sirius red staining in the liver revealed the induction of fibrosis. But the supplementation of AA could induce greater reduction of ethanol induced SIBO, intestinal barrier defects, NF-κB activation and liver fibrosis than silymarin. The possible mechanism may be the inhibitory effect of AA on SIBO, intestinal barrier defect and IKKβ, which decreased the activation of NF-κB and synthesis of cytokines. This might have led to suppression of HSCs activation and liver fibrosis. - Highlights: • Alcohol increases intestinal bacterial overgrowth and permeability of endotoxin. • Endotoxin mediated inflammation plays a major role in alcoholic liver fibrosis. • Ascorbic acid reduces endotoxemia, NF-κB activation and proinflammatory cytokines. • AA's action is by inhibition of SIBO, IKKβ and alteration of

  8. Oracle Management Tool Suite

    Energy Science and Technology Software Center (OSTI)

    2007-06-01

    The Oracle Management Tool Suite is used to automatically manage Oracle based systems. This includes startup and shutdown of databases and application servers as well as backup, space management, workload management and log file management.

  9. Tat-CBR1 inhibits inflammatory responses through the suppressions of NF-κB and MAPK activation in macrophages and TPA-induced ear edema in mice

    SciTech Connect (OSTI)

    Kim, Young Nam; Kim, Dae Won; Jo, Hyo Sang; Shin, Min Jea; Ahn, Eun Hee; Ryu, Eun Ji; Yong, Ji In; Cha, Hyun Ju; Kim, Sang Jin; Yeo, Hyeon Ji; Youn, Jong Kyu; Hwang, Jae Hyeok; Jeong, Ji-Heon; Kim, Duk-Soo; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

    2015-07-15

    Human carbonyl reductase 1 (CBR1) plays a crucial role in cell survival and protects against oxidative stress response. However, its anti-inflammatory effects are not yet clearly understood. In this study, we examined whether CBR1 protects against inflammatory responses in macrophages and mice using a Tat-CBR1 protein which is able to penetrate into cells. The results revealed that purified Tat-CBR1 protein efficiently transduced into Raw 264.7 cells and inhibited lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2), nitric oxide (NO) and prostaglandin E{sub 2} (PGE{sub 2}) expression levels. In addition, Tat-CBR1 protein leads to decreased pro-inflammatory cytokine expression through suppression of nuclear transcription factor-kappaB (NF-κB) and mitogen activated protein kinase (MAPK) activation. Furthermore, Tat-CBR1 protein inhibited inflammatory responses in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation when applied topically. These findings indicate that Tat-CBR1 protein has anti-inflammatory properties in vitro and in vivo through inhibition of NF-κB and MAPK activation, suggesting that Tat-CBR1 protein may have potential as a therapeutic agent against inflammatory diseases. - Highlights: • Transduced Tat-CBR1 reduces LPS-induced inflammatory mediators and cytokines. • Tat-CBR1 inhibits MAPK and NF-κB activation. • Tat-CBR1 ameliorates inflammation response in vitro and in vivo. • Tat-CBR1 may be useful as potential therapeutic agent for inflammation.

  10. The plant limonoid 7-oxo-deacetoxygedunin inhibits RANKL-induced osteoclastogenesis by suppressing activation of the NF-{kappa}B and MAPK pathways

    SciTech Connect (OSTI)

    Wisutsitthiwong, Chonnaree; Buranaruk, Chayanit; Pudhom, Khanitha; Palaga, Tanapat

    2011-11-18

    Highlights: Black-Right-Pointing-Pointer A gedunin type limonoid from seeds of mangroves, 7-oxo-7-deacetoxygedunin, exhibits strong anti-osteoclastogenic activity. Black-Right-Pointing-Pointer Treatment with this limonoid results in significant decrease in expression of NFATc1 and osteoclast-related genes. Black-Right-Pointing-Pointer The mode of action of this limonoid is by inhibiting activation of the NF-{kappa}B and MAPK pathways which are activated by RANKL. -- Abstract: Osteoclasts together with osteoblasts play pivotal roles in bone remodeling. Aberrations in osteoclast differentiation and activity contribute to osteopenic disease. Osteoclasts differentiate from monocyte/macrophage progenitors, a process that is initiated by the interaction between receptor activator of NF-{kappa}B (RANK) and its ligand, RANKL. In this study, we identified 7-oxo-7-deacetoxygedunin (7-OG), a gedunin type limonoid from seeds of the mangrove Xylocarpus moluccensis, as a potent inhibitor of osteoclastogenesis. Additionally, 7-OG showed strong anti-osteoclastogenic activity with low cytotoxicity against the monocyte/macrophage progenitor cell line, RAW264.7. The IC50 for anti-osteoclastogenic activity was 4.14 {mu}M. Treatment with 7-OG completely abolished the appearance of multinucleated giant cells with tartrate-resistant acid phosphatase activity in RAW264.7 cells stimulated with RANKL. When the expression of genes related to osteoclastogenesis was investigated, a complete downregulation of NFATc1 and cathepsin K and a delayed downregulation of irf8 were observed upon 7-OG treatment in the presence of RANKL. Furthermore, treatment with this limonoid suppressed RANKL-induced activation of p38, MAPK and Erk and nuclear localization of NF-{kappa}B p65. Taken together, we present evidence indicating a plant limonoid as a novel osteoclastogenic inhibitor that could be used for osteoporosis and related conditions.

  11. Mangiferin exerts hepatoprotective activity against D-galactosamine induced acute toxicity and oxidative/nitrosative stress via Nrf2NF?B pathways

    SciTech Connect (OSTI)

    Das, Joydeep; Ghosh, Jyotirmoy; Roy, Anandita; Sil, Parames C.

    2012-04-01

    Mangiferin, a xanthone glucoside, is well known to exhibit antioxidant, antiviral, antitumor, anti-inflammatory and gene-regulatory effects. In the present study, we isolated mangiferin from the bark of Mangifera indica and assessed its beneficial role in galactosamine (GAL) induced hepatic pathophysiology. GAL (400 mg/kg body weight) exposed hepatotoxic rats showed elevation in the activities of serum ALP, ALT, levels of triglycerides, total cholesterol, lipid-peroxidation and reduction in the levels of serum total proteins, albumin and cellular GSH. Besides, GAL exposure (5 mM) in hepatocytes induced apoptosis and necrosis, increased ROS and NO production. Signal transduction studies showed that GAL exposure significantly increased the nuclear translocation of NF?B and elevated iNOS protein expression. The same exposure also elevated TNF-?, IFN-?, IL-1?, IL-6, IL-12, IL-18 and decreased IL-10 mRNA expressions. Furthermore, GAL also decreased the protein expression of Nrf2, NADPH:quinine oxidoreductase-1, heme oxygenase-1 and GST?. However, mangiferin administration in GAL intoxicated rats or coincubation of hepatocytes with mangiferin significantly altered all these GAL-induced adverse effects. In conclusion, the hepatoprotective role of mangiferin was due to induction of antioxidant defense via the Nrf2 pathway and reduction of inflammation via NF?B inhibition. Highlights: ?Galactosamine induces hepatocytes death via oxidative and nitrosative stress. ?Mangiferin exerts hepatoprotective effect/antioxidant defense via Nrf2 pathway. ?Mangiferin exerts anti-inflammatory responses by inhibiting NF-?B. ?Mangiferin suppresses galactosamine-induced repression of IL-10 mRNA.

  12. Managing Critical Management Improvement Initiatives

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2003-10-01

    Provides requirements and responsibilities for planning, executing and assessing critical management improvement initiatives within DOE. DOE N 251.59, dated 9/27/2004, extends this Notice until 10/01/2005. Archived 11-8-10. Does not cancel other directives.

  13. HSF1 and NF-κB p65 participate in the process of exercise preconditioning attenuating pressure overload-induced pathological cardiac hypertrophy

    SciTech Connect (OSTI)

    Xu, Tongyi; Zhang, Ben; Yang, Fan; Cai, Chengliang; Wang, Guokun; Han, Qingqi; Zou, Liangjian

    2015-05-08

    Pathological cardiac hypertrophy, often accompanied by hypertension, aortic stenosis and valvular defects, is typically associated with myocyte remodeling and cardiac dysfunction. Exercise preconditioning (EP) has been proven to enhance the tolerance of the myocardium to cardiac ischemia-reperfusion injury. However, the effects of EP in pathological cardiac hypertrophy are rarely reported. 10-wk-old male Sprague–Dawley rats (n = 80) were randomly divided into four groups: sham, TAC, EP + sham and EP + TAC. Two EP groups were subjected to 4 weeks of treadmill training, and the EP + TAC and TAC groups were followed by TAC operations. The sham and EP + sham groups underwent the same operation without aortic constriction. Eight weeks after the surgery, we evaluated the effects of EP by echocardiography, morphology, and histology and observed the expressions of the associated proteins. Compared with the respective control groups, hypertrophy-related indicators were significantly increased in the TAC and EP + TAC groups (p < 0.05). However, between the TAC and EP + TAC groups, all of these changes were effectively inhibited by EP treatment (p < 0.05). Furthermore, EP treatment upregulated the expression of HSF1 and HSP70, increased the HSF1 levels in the nuclear fraction, inhibited the expression of the NF-κB p65 subunit, decreased the NF-κB p65 subunit levels in the nuclear fraction, and reduced the IL2 levels in the myocardia of rats. EP could effectively reduce the cardiac hypertrophic responses induced by TAC and may play a protective role by upregulating the expressions of HSF1 and HSP70, activating HSF1 and then inhibiting the expression of NF-κB p65 and nuclear translocation. - Highlights: • EP could effectively reduce the cardiac hypertrophic responses induced by TAC. • EP may play a protective role by upregulating the expressions of HSF1 and HSP70 and then activating HSF1. • EP may play a protective role by inhibiting the expression

  14. Management Overview

    Office of Environmental Management (EM)

    Department of Energy's Isotope Development and Production for Research and Applications Program's Fiscal Year 2009 Balance Sheet Audit OAS-FS-12-09 June 2012 January 30, 2012 Mr. Gregory Friedman, Inspector General Dr. Jehanne Gillo, Director, Facilities and Project Management Division, Office of Nuclear Physics U.S. Department of Energy Washington, DC 20585 Dear Mr. Friedman and Dr. Gillo: We have audited the balance sheet of the United States Department of Energy's (Department or DOE) Isotope

  15. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Used Fuel Disposition R&D Campaign Working Group Meeting Introduction and Summary Peter Swift National Technical Director Used Fuel Disposition R&D Campaign Las Vegas, Nevada June 7, 2016 Sandia National Laboratories is a multiprogram laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5194 PE Used

  16. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Site Evaluation David C. Sassani Sandia National Laboratories Used Fuel Disposition Campaign Annual Working Group Meeting June 6-9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5400 PE Used Fuel Disposition Presentation Overview  Deep Borehole Field Test

  17. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Disposal: Preliminary Performance Assessment Emily Stein, Geoff Freeze, Kris Kuhlman, Glenn Hammond, Jenn Frederick Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Deep Borehole Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract

  18. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Borehole Characterization Kris Kuhlman Sandia National Laboratories Used Fuel Disposition Working Group Meeting June 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5041 PE Used Fuel Disposition Conceptual Profiles 2 1 2 3 4 5 Depth [km] Sources of Salinity *

  19. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Uranium(VI) Sorption and Diffusion in Montmorillonite and Bentonite: Experiments and Modeling Ruth M. Tinnacher 1 , Christophe Tournassat 2 , James A. Davis 1 1) Earth and Environmental Sciences Area, Lawrence Berkeley National Lab 2) BRGM, French Geological Survey, Orléans, France Used Fuel Disposition Campaign - Annual Meeting Las Vegas, June 8 2016 Used Fuel Disposition 2 Research Motivation The long-term management of nuclear waste requires reliable predictions of radionuclide transport

  20. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Engineering Overview Ernest Hardin, Andrew Clark, John Cochran, Elena Kalinina, Emily Stein and Jiann Su Sandia National Laboratories Fred Peretz Oak Ridge National Laboratory UFD Working Group - Las Vegas, NV June 7-9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

  1. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Waste Package Handling Conceptual Design Process Fred Peretz Oak Ridge National Laboratory UFD Annual Working Group Meeting June 8, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-nnnnn Used Fuel Disposition June 8, 2016 UFD Annual Working Group Meeting 2

  2. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Options for Completing the Emplacement Zone for Deep Borehole Field Disposal John R. Cochran & Ernest L. Hardin Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Deep Borehole Field Test Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under

  3. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    A Reinvestigation into the Isothermal Room Closure Predictions at WIPP Benjamin Reedlunn Sandia National Laboratories Annual UFD Working Group Meeting June 8 th , 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5495 C Used Fuel Disposition Acknowledgements /

  4. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Generic Disposal Systems Analysis (GDSA) Paul Mariner, Glenn Hammond, Emily Stein, David Sevougian, and Jennifer Frederick Sandia National Laboratories 2016 UFD Group Meeting UNLV, Las Vegas, Nevada June 8, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5393

  5. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Simulation Framework: PFLOTRAN Glenn Hammond Sandia National Laboratories 2016 UFDC Annual Working Group Meeting GDSA Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5318 PE Used Fuel Disposition  Petascale reactive

  6. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Isotope Chemistry and Source Term Paul Mariner, Glenn Hammond, and Jennifer Frederick Sandia National Laboratories 2016 UFD Meeting, Las Vegas, Nevada June 8, 2016, GDSA Session Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5392 PE Used Fuel Disposition June 8,

  7. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Radioisotope Source Term Degradation and Implementation in PFLOTRAN Jennifer M. Frederick Glenn E. Hammond and Paul Mariner Sandia National Laboratories 2016 UFDC Annual Working Group Meeting GDSA Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract

  8. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Preview of GDSA/Process Model Integration Session S. David Sevougian Sandia National Laboratories 2016 UFDC Annual Working Group Meeting GDSA Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5294PE. Used Fuel Disposition

  9. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Building the Crystalline Reference Case Performance Assessment Emily Stein, Jenn Frederick, Glenn Hammond, Paul Mariner, Dave Sevougian Sandia National Laboratories 2016 UFDC Annual Working Group Meeting GDSA Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under

  10. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Planning for FY2017 Paul Mariner, Glenn Hammond, Emily Stein, David Sevougian, and Jennifer Frederick Sandia National Laboratories 2016 UFD Group Meeting UNLV, Las Vegas, Nevada June 8, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5389 PE Used Fuel

  11. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Introduction and Objectives for GDSA ⇔ Process Model Integration S. David Sevougian Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Process Model Integration Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

  12. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    PFLOTRAN Process Modeling: Density Dependence on Salinity Glenn Hammond Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Integration Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5319 PE Used Fuel

  13. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Integrating Discrete Fracture Networks with Performance Assessment Emily Stein, Kris Kuhlman Sandia National Laboratories Nataliia Makedonska, Satish Karra, Jeffrey Hyman Los Alamos National Laboratory 2016 UFDC Annual Working Group Meeting GDSA Integration Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's

  14. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Package and Waste Form Degradation and Implementation in PFLOTRAN Jennifer M. Frederick Glenn E. Hammond and Paul Mariner Sandia National Laboratories 2016 UFDC Annual Working Group Meeting GDSA Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract

  15. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Advances in PFLOTRAN Gridding: Octree Refinement and Ghost Node Correction Ayman Alzraiee and Glenn Hammond Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Integration Session, June 8, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract

  16. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Statistical Outputs of Probabilistic Performance Assessment Robert J. MacKinnon Sandia National Laboratories Used Fuel Disposition Working Group Meeting June 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5123 PE Used Fuel Disposition Performance Assessment

  17. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Remaining Process Model Gaps for GDSA Paul Mariner, David Sevougian, Glenn Hammond, Emily Stein, and Jennifer Frederick Sandia National Laboratories 2016 UFD Group Meeting UNLV, Las Vegas, Nevada June 8, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-5391 PE

  18. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    KOSINA Collaboration Ed Matteo UFD Working Group University of Nevada/Las Vegas June 7-9, 2016 Sandia National Laboratories is a multiprogram laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-4049 PE Used Fuel Disposition What is KOSINA? June 9, 2016 KOSINA Collaboration - UFD Working Group Mtg. 2 Used Fuel

  19. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Feature, Event, and Process (FEP) Catalogue for SNF/HLW Disposal in Salt Geoff Freeze, S. David Sevougian, Mike Gross, Kris Kuhlman, Christi Leigh - SNL Jens Wolf, Dieter Buhmann, Jörg Mönig UFD Working Group Meeting Las Vegas, NV June 9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract

  20. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5328PE Overview of Defense Repository Safety Analysis R&D S. David Sevougian Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Defense Repository Session, June 9, 2016 Las Vegas, NV Used

  1. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    D-Repo Performance Assessment: Crystalline Reference Case Emily Stein, Dave Sevougian, Glenn Hammond, Jenn Frederick, Paul Mariner Sandia National Laboratories 2016 UFDC Annual Working Group Meeting Defense Repository Session, June 9, 2016 Las Vegas, NV Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the United States Department of Energy's National Nuclear Security Administration

  2. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Session Summary Geoff Freeze Sandia National Laboratories UFD Working Group Meeting Las Vegas, NV June 9, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-6237 C Used Fuel Disposition 2 Deep Borehole Disposal (DBD) Breakout Sessions SESSION 2 - WEDNESDAY, JUNE 8,

  3. Environmental Management

    ScienceCinema (OSTI)

    None

    2015-01-07

    Another key aspect of the NNSS mission is Environmental Management program, which addresses the environmental legacy from historic nuclear weapons related activities while also ensuring the health and safety of present day workers, the public, and the environment as current and future missions are completed. The Area 5 Radioactive Waste Management site receives low-level and mixed low-level waste from some 28 different generators from across the DOE complex in support of the legacy clean-up DOE Environmental Management project. Without this capability, the DOE would not be able to complete the clean up and proper disposition of these wastes. The program includes environmental protection, compliance, and monitoring of the air, water, plants, animals, and cultural resources at the NNSS. Investigation and implementation of appropriate corrective actions to address the contaminated ground water facilities and soils resulting from historic nuclear testing activities, the demolition of abandoned nuclear facilities, as well as installation of ground water wells to identify and monitor the extent of ground water contamination.

  4. Environmental Management

    SciTech Connect (OSTI)

    2014-11-12

    Another key aspect of the NNSS mission is Environmental Management program, which addresses the environmental legacy from historic nuclear weapons related activities while also ensuring the health and safety of present day workers, the public, and the environment as current and future missions are completed. The Area 5 Radioactive Waste Management site receives low-level and mixed low-level waste from some 28 different generators from across the DOE complex in support of the legacy clean-up DOE Environmental Management project. Without this capability, the DOE would not be able to complete the clean up and proper disposition of these wastes. The program includes environmental protection, compliance, and monitoring of the air, water, plants, animals, and cultural resources at the NNSS. Investigation and implementation of appropriate corrective actions to address the contaminated ground water facilities and soils resulting from historic nuclear testing activities, the demolition of abandoned nuclear facilities, as well as installation of ground water wells to identify and monitor the extent of ground water contamination.

  5. ORISE: Emergency Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management Emergency Management Effective emergency management relies on thorough integration of preparedness plans at all levels of government. The Oak Ridge Institute for Science...

  6. Environmental Management System

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management System Environmental Management System An Environmental Management System (EMS) is a set of processes and practices that enable an organization to reduce its...

  7. M E Environmental Management Environmental Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    safety  performance  cleanup  closure M E Environmental Management Environmental Management safety  performance  cleanup  closure M E Environmental Management Environmental Management M E Environmental Management Environmental Management Office of Site Restoration, EM-10 Office of D&D and Facility Engineering, EM-13 Facility Deactivation & Decommissioning (D&D) D&D Program Map Addendum: Impact of American Recovery and Reinvestment Act (ARRA) on EM's D&D

  8. Best Management Practice #1: Water Management Planning

    Office of Energy Efficiency and Renewable Energy (EERE)

    A successful water management program starts with developing a comprehensive water management plan. This plan should be included within existing facility operating plans.

  9. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Safety Case Framework Geoff Freeze Sandia National Laboratories UFD Working Group Meeting Las Vegas, NV June 8, 2016 Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000. SAND2016-5351PE Used Fuel Disposition 2 Safety Case Overview "The safety case is an integration of

  10. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    On-Line Waste Library (OWL) Walter Walkow Solutions Architect, Data Sciences, SNL Database and Capabilities June 7, 2016 SAND2016-5415 C Used Fuel Disposition June 2016 On-Line Waste Library (OWL) - Database & Capabilities SAND2016-5415 C 2 What is OWL, What objective does it support?  OWL - The on-line waste library (OWL) contains detailed cross- linked information, both technical and organizational, regarding DOE-managed high-level waste (HLW) and spent nuclear fuel (SNF) (D-wastes),

  11. Management Overview

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    package degradation: Clay - Metal Interactions Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04- 94AL85000. SAND2016-nnnnn Carlos F. Jové Colón Sandia National Laboratories Florie A. Caporuscio Los Alamos National Laboratory Las Vegas, Nevada - June 7 - 9, 2016 SAND2016-5247 PE Used

  12. TGF-{beta}1 increases invasiveness of SW1990 cells through Rac1/ROS/NF-{kappa}B/IL-6/MMP-2

    SciTech Connect (OSTI)

    Binker, Marcelo G.; CBRHC Research Center, Buenos Aires ; Binker-Cosen, Andres A.; Gaisano, Herbert Y.; Cosen, Rodica H. de; Cosen-Binker, Laura I.

    2011-02-04

    Research highlights: {yields} Rac1 mediates TGF-{beta}1-induced SW1990 invasion through MMP-2 secretion and activation. {yields} NADPH-generated ROS act downstream of Rac1 in TGF-{beta}1-challenged SW1990 cells. {yields} TGF-{beta}1-stimulated ROS activate NF-{kappa}B in SW1990 cells. {yields} NF{kappa}B-induced IL-6 release is required for secretion and activation of MMP-2 in SW1990 cells. -- Abstract: Human pancreatic cancer invasion and metastasis have been found to correlate with increased levels of active matrix metalloproteinase 2 (MMP-2). The multifunctional cytokine transforming growth factor beta 1 (TGF-{beta}1) has been shown to increase both secretion of MMP-2 and invasion by several pancreatic cancer cell types. In the present study, we investigated the signaling pathway involved in TGF-{beta}1-promoted MMP-2 secretion and invasion by human pancreatic cancer cells SW1990. Using specific inhibitors, we found that stimulation of these tumor cells with TGF-{beta}1 induced secretion and activation of the collagenase MMP-2, which was required for TGF-{beta}1-stimulated invasion. Our results also indicate that signaling events involved in TGF-{beta}1-enhanced SW1990 invasiveness comprehend activation of Rac1 followed by generation of reactive oxygen species through nicotinamide adenine dinucleotide phosphate-oxidase, activation of nuclear factor-kappa beta, release of interleukin-6, and secretion and activation of MMP-2.

  13. 2016 DOE Project Management Workshop - "Enhancing Project Management...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    6 DOE Project Management Workshop - "Enhancing Project Management" 2016 DOE Project Management Workshop - "Enhancing Project Management" 20160407-doe-project-management-workshop-AD...

  14. Risk Management Process Overview

    Broader source: Energy.gov [DOE]

    The cybersecurity risk management process explained in the Electricity Sector Cybersecurity Risk Management Process (RMP) Guideline has two primary components: the risk management model and the the risk management cycle.

  15. Your Records Management Responsibilities

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Your Records Management Responsibilities Table of Contents INTRODUCTION RECORDS MANAGEMENT IN THE FEDERAL GOVERNMENT RECORDS MANAGEMENT IN THE DEPARTMENT OF ENERGY IMPORTANCE OF RECORDS MANAGEMENT YOUR RECORDS MANAGEMENT RESPONSIBILITIES RECORDS MANAGEMENT LIFE CYCLE ELECTRONIC RECORDS & RECORDKEEPING LAW, REGULATION, AND POLICY ASSISTANCE RECORDS MANAGEMENT TERMS 2 INTRODUCTION If you are a government employee or contractor working for a federal agency, records management is part of your

  16. Energy Management and Financing

    Office of Energy Efficiency and Renewable Energy (EERE)

    This Tuesday Webcast for Industry covers how to become a Certified Energy Manager and Certified Practitioner in Energy Management Systems

  17. Integrated Safety Management Policy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    INTEGRATED SAFETY MANAGEMENT SYSTEM DESCRIPTION U.S. DEPARTMENT OF ENERGY Office of Environmental Management Headquarters May 2008 Preparation: Braj K. sin& Occupational Safety and Health Manager Office of Safety Management Concurrence: Chuan-Fu wu Director, Offlce of Safety Management Deputy Assistant Secretary for safe& Management andoperations Operations Officer for 1 Environmental Management Approval: Date p/-g Date Environmental Management TABLE OF CONTENTS

  18. Integrated Safety Management and Environmental Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Laboratory | Department of Energy Integrated Safety Management Workshop Registration, PIA, Idaho National Laboratory Integrated Safety Management Workshop Registration, PIA, Idaho National Laboratory Integrated Safety Management Workshop Registration, PIA, Idaho National Laboratory Integrated Safety Management Workshop Registration, PIA, Idaho National Laboratory (265.91 KB) More Documents & Publications TRAIN-PIA.pdf Occupational Medicine - Assistant PIA, Idaho National Laboratory PIA -

  19. John Angelis named Manager, Information Resource Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Names John S. Angelis Manager of Information Resource Management CARLSBAD, N.M., March 22, 2000 - The Westinghouse Waste Isolation Division (WID) has named John S. Angelis of Carlsbad as Manager of Information Resource Management at the Waste Isolation Pilot Plant (WIPP). "John's extensive computer technology and telecommunications experience, combined with his progressive attitude, make him an ideal choice for this increasingly important position," said WID General Manager Joe

  20. Cyanidin-3-glucoside inhibits UVB-induced oxidative damage and inflammation by regulating MAP kinase and NF-κB signaling pathways in SKH-1 hairless mice skin

    SciTech Connect (OSTI)

    Pratheeshkumar, Poyil; Son, Young-Ok; Wang, Xin; Divya, Sasidharan Padmaja; Joseph, Binoy; Hitron, John Andrew; Wang, Lei; Kim, Donghern; Yin, Yuanqin; Roy, Ram Vinod; Lu, Jian; Zhang, Zhuo; Wang, Yitao; and others

    2014-10-01

    Skin cancer is one of the most commonly diagnosed cancers in the United States. Exposure to ultraviolet-B (UVB) radiation induces inflammation and photocarcinogenesis in mammalian skin. Cyanidin-3-glucoside (C3G), a member of the anthocyanin family, is present in various vegetables and fruits especially in edible berries, and displays potent antioxidant and anticarcinogenic properties. In this study, we have assessed the in vivo effects of C3G on UVB irradiation induced chronic inflammatory responses in SKH-1 hairless mice, a well-established model for UVB-induced skin carcinogenesis. Here, we show that C3G inhibited UVB-induced skin damage and inflammation in SKH-1 hairless mice. Our results indicate that C3G inhibited glutathione depletion, lipid peroxidation and myeloperoxidation in mouse skin by chronic UVB exposure. C3G significantly decreased the production of UVB-induced pro-inflammatory cytokines, such as IL-6 and TNF-α, associated with cutaneous inflammation. Likewise, UVB-induced inflammatory responses were diminished by C3G as observed by a remarkable reduction in the levels of phosphorylated MAP kinases, Erk1/2, p38, JNK1/2 and MKK4. Furthermore, C3G also decreased UVB-induced cyclooxygenase-2 (COX-2), PGE{sub 2} and iNOS levels, which are well-known key mediators of inflammation and cancer. Treatment with C3G inhibited UVB-induced nuclear translocation of NF-κB and degradation of IκBα in mice skin. Immunofluorescence assay revealed that topical application of C3G inhibited the expression of 8-hydroxy-2′-deoxyguanosine, proliferating cell nuclear antigen, and cyclin D1 in chronic UVB exposed mouse skin. Collectively, these data indicates that C3G can provide substantial protection against the adverse effects of UVB radiation by modulating UVB-induced MAP kinase and NF-κB signaling pathways. - Highlights: • C3G inhibited UVB-induced oxidative damage and inflammation. • C3G inhibited UVB-induced COX-2, iNOS and PGE{sub 2} production. • C3G

  1. ENVIRONMENTAL MANAGEMENT OFFICE OF ENVIRONMENTAL MANAGEMENT

    Office of Environmental Management (EM)

    ENVIRONMENTAL MANAGEMENT OFFICE OF ENVIRONMENTAL MANAGEMENT OFFICE OF ENVIRONMENTAL MANAGEMENT OFFICE OF EM Recovery NEWS FLASH RECOVERY.GOV March 10, 2011 American Recovery and Reinvestment Act Payments Surge Past $4 Billion U.S. Depar tment of Energy | Office of Environmental Management For More Information on EM Recovery Act Work, Visit Us on the Web: http://www.em.doe.gov/emrecovery/ FINANCIAL HIGHLIGHTS * More than $4 BILLION in Recovery Act payments are accelerating environmental cleanup

  2. Best Management Practice #1: Water Management Planning | Department...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1: Water Management Planning Best Management Practice 1: Water Management Planning A successful water management program starts with a comprehensive strategic plan. The process ...

  3. DOE Jobs Online (Hiring Manager), Office of Human Capitol Management...

    Broader source: Energy.gov (indexed) [DOE]

    Jobs Online (Hiring Manager), Office of Human Capitol Management Innovation and Solutions DOE Jobs Online (Hiring Manager), Office of Human Capitol Management Innovation and ...

  4. AWWA Utility Management Conference

    Broader source: Energy.gov [DOE]

    Hosted by the American Water Works Association (AWWA), the Utility Management Conference is one of the leading management conferences to share experiences and learn from others in similar situations to the most pressing management issues of the day.

  5. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    Second Quarter Overall Contract and Project Management Improvement Performance Metrics and Targets 1 ContractProject Management Performance Metric FY 2012 Target FY 2012 Forecast ...

  6. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fourth Quarter Overall Contract and Project Management Improvement Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2011 Target FY 2011 ...

  7. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    2 nd Quarter Overall Contract and Project Management Performance Metrics and Targets ContractProject Management Performance Metrics FY 2009 Target FY 2009 Actual Comment 1. ...

  8. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    3 rd Quarter Overall Contract and Project Management Performance Metrics and Targets ContractProject Management Performance Metrics FY 2009 Target FY 2009 Actual Comment 1. ...

  9. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fourth Quarter Overall Contract and Project Management Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2010 Target FY 2010 Actual FY ...

  10. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    Third Quarter Overall Contract and Project Management Improvement Performance Metrics and Targets 1 ContractProject Management Performance Metric FY 2012 Target FY 2012 Forecast ...

  11. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Fourth Quarter Overall Contract and Project Management Improvement Performance Metrics and Targets 1 ContractProject Management Performance Metric FY 2012 Target FY 2012 Final FY ...

  12. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Third Quarter Overall Contract and Project Management Improvement Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2011 Target FY 2011 ...

  13. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    Second Quarter Overall Contract and Project Management Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2010 Target FY 2010 Actual FY ...

  14. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    1 st Quarter Overall Contract and Project Management Performance Metrics and Targets ContractProject Management Performance Metrics FY 2009 Target FY 2009 Actual Comment 1. ...

  15. Contract/Project Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    8 4 th Quarter Metrics Final Overall Contract and Project Management Performance Metrics and Targets ContractProject Management Performance Metrics FY 2008 Target FY 2008 Actual ...

  16. Turbine Thermal Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Turbine Thermal Management Fact Sheets Research Team Members Key Contacts Turbine Thermal Management The gas turbine is the workhorse of power generation, and technology advances ...

  17. Data Management Policy The

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management Policy The guidelines below describe Data Management procedures, processes and resources that need to be understood by both user projects and in-house research. 1....

  18. Supply Management Specialist

    Broader source: Energy.gov [DOE]

    This position is located in the Logistics Management organization (NSL), Supply Chain Services (NS), Chief Administrative Office (N). NSL manages the warehousing of materials; the investment...

  19. COMPREHENSIVE LEGACY MANAGEMENT

    Office of Legacy Management (LM)

    Fernald Preserve, Fernald, Ohio Comprehensive Legacy Management and Institutional Controls ... blank LMSFERS03496-8.0 Comprehensive Legacy Management and Institutional Controls ...

  20. End Points Management

    Broader source: Energy.gov [DOE]

    The policy of the EM is that a formal project management approach be used for the planning, managing, and conducting of its projects.

  1. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    Third Quarter Overall Contract and Project Management Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2010 Target FY 2010 Forecast FY ...

  2. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    Contract and Project Management Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2011 Target FY 2011 Actual & Forecast FY 2011 Pre- & ...

  3. Contract/Project Management

    Broader source: Energy.gov (indexed) [DOE]

    and Project Management Improvement Performance Metrics and Targets 1 ContractProject Management Primary Performance Metrics FY 2011 Target FY 2011 Forecast FY 2011 Pre- & ...

  4. Technical Standards Managers

    Broader source: Energy.gov (indexed) [DOE]

    FACILITYADDRESS LOC CODE DOE TECHNICAL STANDARD MANAGERS AU-30 DOE Technical Standards ... FACILITYADDRESS LOC CODE DOE TECHNICAL STANDARD MANAGERS DOE-CTA TSM Gustave E. (Bud) ...

  5. Management of Nuclear Materials

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2009-08-17

    To establish requirements for the lifecycle management of DOE owned and/or managed accountable nuclear materials. Cancels DOE O 5660.1B.

  6. Management Control Cover

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    OAS-M-06-02 November 2005 REPORT ON MANAGEMENT CONTROLS OVER ASSESSING NATURAL ... and Methodology 5 Prior Audit Reports 6 Management Comments 7 NATURAL RESURCE DAMAGE ...

  7. Safety Management System Policy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    SAFETY MANAGEMENT POLICY PURPOSE AND SCOPE To establish the Department of Energy's (DOE) expectation for safety, 1 including integrated safety management that will enable ...

  8. Ross Management | Open Energy Information

    Open Energy Info (EERE)

    Management Jump to: navigation, search Name: Ross Management Place: Goldendale, Washington State Product: Ross Management is the management parent for a group of family owned...

  9. Chemical Management Contacts

    Broader source: Energy.gov [DOE]

    Contacts for additional information on Chemical Management and brief description on Energy Facility Contractors Group

  10. Managing Your User Account

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Managing Your Account Managing Your User Account Use the NERSC Information Management (NIM) system to customize your user account and keep your personal information up-to-date. See the NIM User's Guide, especially the "Managing Your User Account with NIM" section. Last edited: 2016-04-29 11:35:06

  11. Environmental Management System

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management System Environmental Management System An Environmental Management System is a systematic method for assessing mission activities, determining the environmental impacts of those activities, prioritizing improvements, and measuring results. May 30, 2012 The continuous improvement cycle Our Environmental Management System encourages continuous improvement of our environmental performance. Contact Environmental Communication & Public Involvement P.O. Box 1663 MS M996 Los Alamos, NM

  12. Manager`s views of public involvement

    SciTech Connect (OSTI)

    Branch, K.M.; Heerwagen, J.; Bradbury, J.

    1995-12-01

    Four issues commonly form the framework for debates about the acceptability of proposed projects or technologies--the substantive decision or technological choice; the treatment of the community by the proponent organization; the way the decision-making process has been structured and managed; and the status of institutional safeguards and protection. One of the clear messages of cultural theory is that differences in perspectives are a normal and inevitable part of society, and that attempts to resolve differences by persuasion are not likely to work. These findings are useful when considering the goals and possibilities of public involvement as a decision-making tool, and when designing or evaluating public involvement training programs for managers. The research reported here examines the viewpoints and concerns of managers and decision-makers about the four issues identified above, with particular emphasis on their perspectives and concerns about opening decision-making processes to the public and about managers` roles and responsibilities for structuring and managing open decision-making processes. Implications of these findings for public involvement training for managers is also discussed. The data presented in this paper were obtained from face-to-face interviews with managers and decision-makers with experience managing a variety of hazardous waste management decision-making processes. We conducted these interviews in the course of four separate research projects: needs assessments to support the design and development of a public involvement training program for managers; a study of community residents` and managers` perspectives on the chemical stockpile disposal program; an evaluation of the effectiveness of public involvement training for managers in the Department of Energy; and a study to develop indicators of the benefits and costs of public involvement.

  13. 2014 DOE Project Management Workshop

    Broader source: Energy.gov [DOE]

    What:  2014 DOE Project Management Workshop (Meeting the Challenge—Integrated Acquisition & Project Management)

  14. The (3He,tf) as a surrogate reaction to determine (n,f) cross sections in the 10 to 20 MeV energy range

    SciTech Connect (OSTI)

    Basunia, M. S.; Clark, R. M.; Goldblum, B. L.; Bernstein, L. A.; Phair, L.; Burke, J. T.; Beausang, C. W.; Bleuel, D. L.; Darakchieva, B.; Dietrich, F. S.; Evtimova, M.; Fallon, P.; Gibelin, J.; Hatarik, R.; Jewett, C. C.; Lesher, S. R.; McMahan, M. A.; Rodriguez-Vieitez, E.; Wiedeking, M.

    2009-02-25

    The surrogate reaction 238U(3He,tf) is used to determine the 237Np(n,f) cross section indirectly over an equivalent neutron energy range from 10 to 20 MeV. A self-supporting ~;;761 mu g/cm2 metallic 238U foil was bombarded with a 42 MeV 3He2+ beam from the 88-Inch Cyclotron at Lawrence Berkeley National Laboratory (LBNL). Outgoing charged particles and fission fragments were identified using the Silicon Telescope Array for Reaction Studies (STARS), consists of two 140 mu m and one 1000 mu m Micron S2 type silicon detectors. The 237Np(n,f) cross sections, determined indirectly, were compared with the 237Np(n,f) cross section data from direct measurements, the Evaluated Nuclear Data File (ENDF/B-VII.0), and the Japanese Evaluated Nuclear Data Library (JENDL 3.3) and found to closely follow those datasets. Use of the (3He,tf) reaction as a surrogate to extract (n,f) cross section in the 10 to 20 MeV equivalent neutron energy is found to be suitable.

  15. Project Manager's Guide to Managing Impact and Process Evaluation...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Project Manager's Guide to Managing Impact and Process Evaluation Studies This report provides a step-by-step approach to help managers of EERE evaluation projects create and ...

  16. Manager's Desk Reference on Human Capital Management Flexibilities...

    Broader source: Energy.gov (indexed) [DOE]

    that can be used in day-to-day human capital management activities, especially ... Manager's Desk Reference on Human Capital Management Flexibilities (640.69 KB) Responsible ...

  17. Risk Management Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-01-12

    The purpose of this guide is to describe effective risk management processes. The continuous and iterative process includes updating project risk documents and the risk management plan and emphasizes implementation communication of the risks and actions taken.

  18. Information Technology Project Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2012-12-03

    The Order provides program and project management direction for the acquisition and management of IT projects, investments, and initiatives. Cancels DOE G 200.1-1. Admin Chg 1 approved 1-16-2013.

  19. Risk Management Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-01-18

    This Guide provides non-mandatory risk management approaches for implementing the requirements of DOE O 413.3B, Program and Project Management for the Acquisition of Capital Assets. Supersedes DOE G 413.3-7.

  20. Management Associate I

    Broader source: Energy.gov [DOE]

    A successful candidate in this position will serve as an analyst and advisor to an organization's manager and other managers within the organization within BPA. The position serves as a point of...

  1. Information Technology Project Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2012-12-03

    The Order provides program and project management direction for the acquisition and management of IT projects, investments, and initiatives. Admin Chg 1, dated 1-16-2013, supersedes DOE O 415.1.

  2. Stage Gate Management Guide

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Stage Gate Management in the Biomass Program February 2005 Revision 2 2 TABLE OF CONTENTS OVERVIEW............................................................................................................................. 4 STAGE GATE MANAGEMENT .................................................................................................... 4 STAGE GATE PROCESS AND LONG RANGE STRATEGIC PROGRAM PLANNING ........................ 5 GATE REVIEWS

  3. Management Control Cover

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    OAS-M-06-01 November 2005 REPORT ON MANAGEMENT CONTROLS OVER THE HANFORD SITE ... Project Management ORP did not fully identify and mitigate risks associated with the TRUM ...

  4. Women of Waste Management

    Broader source: Energy.gov [DOE]

    PHOENIX - For the seventh year at the Waste Management Conference, EM contractor Fluor hosted a discussion on the expanding role of women in environmental management this month in a panel session attended by more than 250 people.

  5. Management of Nuclear Materials

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2009-08-17

    To establish requirements for the lifecycle management of DOE owned and/or managed accountable nuclear materials. Admin Chg 1 dated 4-10-2014, supersedes DOE O 410.2.

  6. ENVIRONMENTAL MANAGEMENT ADVISORY BOARD

    Office of Environmental Management (EM)

    Manager | Department of Energy Webinar: Introducing Waste & Materials Tracking in Portfolio Manager ENERGY STAR Webinar: Introducing Waste & Materials Tracking in Portfolio Manager September 15, 2016 1:00PM to 2:00PM EDT Hosted by the U.S. Environmental Protection Agency's (EPA's) ENERGY STAR, this webinar will show how to track your building's waste and materials management. It will introduce the types of waste and disposal methods attendees can track, new metrics and reports, and

  7. emergency management systems

    National Nuclear Security Administration (NNSA)

    9%2A en Building International Emergency Management Systems http:nnsa.energy.govaboutusourprogramsemergencyoperationscounterterrorisminternationalprograms-1

  8. System Management Software

    Energy Science and Technology Software Center (OSTI)

    2013-02-22

    MacPatch is Mac OS X system management software solution. It's used for patching, software distribution and inventory.

  9. Energy Manager Webinar Series

    Broader source: Energy.gov [DOE]

    Energy Managers from leading manufacturing companies share lessons learned from implementing energy savings projects in these one-hour webinars. Topics include real-world challenges like creating a climate for successful project implementation, gaining management support, and obtaining financing. Each webinar highlights a different topic and features an energy manager from a different Better Plants Partner.

  10. Energy, Data Management, Reporting

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Design the Strategy | Deliver Efficiency | Sustain Results Energy, Data Management, Reporting This presentation does not contain any proprietary, confidential, or otherwise restricted information. - - Agenda ● About Schneider Electric ● Enterprise wide Data Management ● Outputs ● Foundation and results ● Part of a complete energy management solution Schneider Electric Sustainability Services 2014 2 - - Schneider Electric Sustainability Services 2014 3 Schneider Electric - the global

  11. Financial Management Oversight

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2006-08-31

    The Order defines requirements for effective financial management and adherence to DOE and applicable external financial management requirements and sets forth standards for ensuring the integrity and responsiveness of financial management and the accuracy and reliability of DOEs financial statements. Supersedes DOE O 2200.13.

  12. ARM - Metadata Management Tool

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FormsMetadata Management Tool Ingest Ingest Workflow Graphic Supporting Workflow Documentation Supporting Tools and Forms Process Configuration Manager (PCM) Metadata Management Tool (MMT) ARM Data Integrator (ADI) Ingest Readiness Form Associated Status Reports Contacts Engineering Processes Engineering Home Workflow Graphic Engineering Workflow Document Tools for Workflow ECR ECO BCR Ingests Value-Added Products Reprocessing Instruments Data System Elements Field Campaign Startup Metadata

  13. ARM - Process Configuration Manager

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    FormsProcess Configuration Manager Ingest Ingest Workflow Graphic Supporting Workflow Documentation Supporting Tools and Forms Process Configuration Manager (PCM) Metadata Management Tool (MMT) ARM Data Integrator (ADI) Ingest Readiness Form Associated Status Reports Contacts Engineering Processes Engineering Home Workflow Graphic Engineering Workflow Document Tools for Workflow ECR ECO BCR Ingests Value-Added Products Reprocessing Instruments Data System Elements Field Campaign Startup Process

  14. Executive Branch Management Scorecard | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Branch Management Scorecard Executive Branch Management Scorecard PDF icon Executive Branch Management Scorecard More Documents & Publications Executive Branch Management Scorecard ...

  15. Environmental Management Advisory Board (EMAB) | Department of...

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Environmental Management Advisory Board (EMAB) Environmental Management Advisory Board (EMAB) Environmental Management Advisory Board (EMAB) Environmental Management Advisory Board ...

  16. Program Management | Department of Energy

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Management Program Management Safety Safety The Office of Environmental Management's (EM) top priority is to ensure proper implementation and continuous improvement of Integrated ...

  17. Managing a Process Safety Management inspection

    SciTech Connect (OSTI)

    Mulvey, N.P. [AcuTech Consulting, Inc., Princeton, NJ (United States)

    1995-12-31

    The Occupational Safety and Health Administration (OSHA) Process Safety Management (PSM) regulations (29 CFR {section} 1910.119) have been in effect for almost three years. State level risk management programs, in New Jersey, California, and Delaware have been in effect for over six years. More recently, Nevada and Louisiana have enacted process safety management programs. These regulations have had a significant impact on all phases of plant operations, including preliminary design, construction, startup, and operations. Through proper planning and the commitment of resources, many facilities are beginning to realize the benefits of well developed PSM programs. This paper will discuss in greater detail some of these benefits, and in particular, the subject of governmental inspections of facilities for process safety management programs.

  18. Multimedia environmental management

    SciTech Connect (OSTI)

    Soesilo, J.A.; Wiley, W.D.

    1999-09-01

    This book explores and supports the argument that effective environmental management must be based on a multimedia approach, which focuses simultaneously on air, water, and waste and enables managers to assess the resulting financial, operation, and management benefits. The multimedia approach, which can be used to design an effective compliance program, includes proper waste and material handling management, systematic monitoring, and record keeping requirements. This approach integrates a wide array of environmental requirements and decision processes, which the authors examine in sixteen chapters, organized into four parts: the role of environmental management; environmental aspects of business operation, environmental processes; and environmental management trends. Within these parts, the authors highlight the development of modern environmental management and provide an overview of federal laws pertinent to multimedia environmental management. They examine such issues as chemical storage and transportation, tank system operations and requirements, waste determination, spill response procedures, and employee training. Environmental processes addressed in the book include the management of solid and hazardous waste, wastewater treatment systems, stormwater management, air emission control, and site remediation. The authors also briefly discuss significant initiatives in US environmental management and look toward corporate sustainable development.

  19. DOE - Office of Legacy Management -- Fernald Environmental Management...

    Office of Legacy Management (LM)

    Fernald Environmental Management Project - 027 FUSRAP Considered Sites Site: Fernald Environmental Management Project (027) Designated Name: Alternate Name: Location: Evaluation ...

  20. ORO Names New Assistant Manager for Environmental Management

    Broader source: Energy.gov [DOE]

    The U.S. Department of Energy Oak Ridge Office names John Eschenberg as its Assistant Manager for Environmental Management.

  1. DOE Jobs Online (Hiring Manager), Office of Human Capitol Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Innovation and Solutions | Department of Energy Jobs Online (Hiring Manager), Office of Human Capitol Management Innovation and Solutions DOE Jobs Online (Hiring Manager), Office of Human Capitol Management Innovation and Solutions DOE Jobs Online (Hiring Manager), Office of Human Capitol Management Innovation and Solutions DOE Jobs Online (Hiring Manager), Office of Human Capitol Management Innovation and Solutions (1.46 MB) More Documents & Publications PIA - GovTrip (DOE data) LM

  2. DOE Office of Environmental Management Project and Contract Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Improvement Timeline | Department of Energy Acquisition » DOE Office of Environmental Management Project and Contract Management Improvement Timeline DOE Office of Environmental Management Project and Contract Management Improvement Timeline Improving DOE contract and project management is a top priority of the Department's senior management and entire organization. View some highlights of EM's improvement actions here. DOE Office of Environmental Management Contract and Project Management

  3. Strategies for Successful Energy Management

    Broader source: Energy.gov [DOE]

    This presentation, given through the DOE's Technical Assitance Program (TAP), provides information on energy management for the portfolio manager initiative

  4. Breakout Group 3: Water Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    3: Water Management Participants Name Organization Tom Benjamin Argonne National ... National Laboratory Breakout Group 3: Water Management GAPSBARRIERS The Water ...

  5. NREL Announces Management Changes

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Announces Management Changes For more information contact: Bob Noun (303) 275-3062 Golden, Colo., June 27, 1997 National Renewable Energy Laboratory (NREL) Director Richard Truly announced a reorganization that creates two new management positions at the laboratory. Effective July 1, 1997, Dr. Stanley Bull becomes Associate Director for Research Operations. He will manage the research and development of more than 30 renewable energy technologies such as solar energy, wind energy, fuels

  6. Configuration Management Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2003-10-27

    The purpose of this standard is to define the objectives of a configuration management process for DOE nuclear facilities (including activities and operations), and to provide detailed examples and supplementary guidance on methods of achieving those objectives. Configuration management is a disciplined process that involves both management and technical direction to establish and document the design requirements nd the physical configuration of the nuclear facility and to ensure that they remain consistent with each other and the documentation.

  7. Office of Personnel Management

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Personnel Management October 2014 Questions & Answers 1 Pathways Programs 5 CFR Parts 213, 302, 315, and 362 Questions and Answers Background Executive Order 13562 Recruiting and Hiring Students and Recent Graduates dated December 27, 2010, authorized two new excepted service hiring authorities and consolidated them with a revised Presidential Management Fellows Program to establish the Pathways Programs for students and recent graduates. The U.S. Office of Personnel Management (OPM)

  8. Land Management - Hanford Site

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Land Management About Us About Hanford Cleanup Hanford History Hanford Site Wide Programs Hanford Cultural Resources Contact Us Land Management Email Email Page | Print Print Page | Text Increase Font Size Decrease Font Size Hanford Site - Hanford Reach The U.S. Department of Energy (DOE) Richland Operations Office (RL) is responsible for the management of Hanford Site property. RL has issued the Mission Support Contract (MSC) to provide direct support to RL, DOE Office River Protection (ORP)

  9. Digital Data Management Plans

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    energy physics through experiments that strengthen our fundamental understanding of matter, energy, space, and time. Plans HAWC gamma-Ray Observatory Data Management Plan (pdf)...

  10. Comprehensive Emergency Management System

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2005-11-02

    The Order establishes policy and assigns roles and responsibilities for the Department of Energy (DOE) Emergency Management System. Supersedes DOE O 151.1B.

  11. Grants Management Specialist

    Broader source: Energy.gov [DOE]

    The Office of Science manages fundamental research programs in basic energy sciences, biological and environmental sciences, and computational science. In addition, the Office of Science is the...

  12. Enterprise Risk Management Framework

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Framework The Enterprise Risk Management (ERM) framework includes four steps: identify the risks, determine the probability and impact of each one, identify controls that are...

  13. Financial Management Handbook - Complete

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    ... Management of Budgetary Resources. (1) Appropriations. ... The apportionment process is detailed in DOE M 135.1-1A, ... and reporting all business transactions under the ...

  14. Managing Large Capital Projects

    Broader source: Energy.gov [DOE]

    Presentation from the 2015 DOE National Cleanup Workshop by Ken Picha, Deputy Assistant Secretary for Tank Waste and Nuclear Material, Office of Environmental Management.

  15. Radioactive Waste Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    1984-02-06

    To establish policies and guidelines by which the Department of Energy (DOE) manages tis radioactive waste, waste byproducts, and radioactively contaminated surplus facilities.

  16. Records Management Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2006-02-03

    The Order sets forth requirements and responsibilities for implementing and maintaining a cost-effective records management program throughout the Department of Energy.

  17. Change Control Management Guide

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-07-29

    The Guide provides a suggested approach and uniform guidance for managing project and contract changes through applying the requirements of DOE O 413.3B. No cancellation.

  18. Records Management Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2011-07-26

    The Order sets forth requirements and responsibilities for establishing and maintaining a program for the efficient and economical management of records and information assets.

  19. Project Management Practices

    Broader source: Energy.gov (indexed) [DOE]

    Version) See Figure 3-3. 19. Administrative Controls 1. Provisions relating to organization and management, procedures, recordkeeping, assessment, and reporting necessary to...

  20. Office of Legacy Management

    Office of Legacy Management (LM)

    Energy Office of Legacy Management JUL 1 0 2008 Alonso Ramirez, Scientific Director EI Verde Research Station Institute for Tropical Ecosystem Studies University of Puerto Rico...

  1. Dreissenid Mussel Prevention, Management...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management, Research, Coordination, and Outreach for the Columbia River Basin A Roadmap to Make Strategic Investments in Federal Columbia River Power System and Technology...

  2. Dreissenid Mussel Prevention, Management, ...

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management, Research, Coordination, and Outreach for the Columbia River Basin A Roadmap to Make Strategic Investments in Federal Columbia River Power System and Technology...

  3. EFRC Management Reference Document

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    EFRC management reference document Energy Frontier Research Centers Acknowledgments of Support (v.1, October 2009) Office of Basic Energy Sciences Office of Science US Department ...

  4. Waste management progress report

    SciTech Connect (OSTI)

    1997-06-01

    During the Cold War era, when DOE and its predecessor agencies produced nuclear weapons and components, and conducted nuclear research, a variety of wastes were generated (both radioactive and hazardous). DOE now has the task of managing these wastes so that they are not a threat to human health and the environment. This document is the Waste Management Progress Report for the U.S. Department of Energy dated June 1997. This progress report contains a radioactive and hazardous waste inventory and waste management program mission, a section describing progress toward mission completion, mid-year 1997 accomplishments, and the future outlook for waste management.

  5. International Commitments Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2008-11-18

    This Order establishes a process to manage the Department's International Commitments under the administrative direction of the Office of Policy and International Affairs. No cancellation.

  6. Logistics Management Specialist

    Broader source: Energy.gov [DOE]

    The Deputy Assistant Secretary for the Office of Petroleum Reserves (OPR) manages the Strategic Petroleum Reserve (SPR) and is responsible for establishing policies, goals, and priorities for the...

  7. E M Environmental Management

    Office of Environmental Management (EM)

    E M Environmental Management safety performance cleanup closure EM RECOVERY ACT TOP LINE MESSAGES * The Department estimates the 6 billion Recovery Act investment has allowed us ...

  8. Managing Increased Charging Demand

    Office of Energy Efficiency and Renewable Energy (EERE) Indexed Site

    Managing Increased Charging Demand Carrie Giles ICF International, Supporting the Workplace Charging Challenge Workplace Charging Challenge Do you already own an EV? Are you...

  9. Information Technology Management

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2013-11-21

    This revised Order is needed to clarify the roles and responsibilities, policies, and procedures for effectively managing IT investments to ensure mission success.

  10. Advisory Committee Management Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2007-10-22

    The Manual provides detailed DOE requirements, responsibilities, processes, and procedures for the establishment, operation, and management of advisory committees. Supersedes DOE M 510.1-1.

  11. High Performance Energy Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Performance Energy Management Reduce energy use and meet your business objectives By applying continuous improvement practices similar to Lean and Six Sigma, the BPA Energy Smart...

  12. Asset Management Strategies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Efficiency and Fish and Wildlife asset categories. The CAB, in consultation with affected business units and the Asset Management Executive Sponsors, determines whether and how...

  13. Management's Discussion & Analysis Profile

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    in conformity with generally accepted accounting principles (GAAP) requires management to make estimates and assumptions that affect the reported amounts of assets and...

  14. Management's Discussion & Analysis Profile

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Adjusted Net Revenue is net revenue after removing the effects of certain debt management actions, in particular the Debt Service Reassignment, from prior years. These debt...

  15. Asset Management Strategies

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Asset-Management-Strategies Sign In About | Careers | Contact | Investors | bpa.gov Search News & Us Expand News & Us Projects & Initiatives Expand Projects & Initiatives...

  16. Efficient Water Use & Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Water Use Goal 4: Efficient Water Use & Management Aware of the arid climate of northern New Mexico, water reduction and conservation remains a primary concern at LANL. Energy...

  17. Group key management

    SciTech Connect (OSTI)

    Dunigan, T.; Cao, C.

    1997-08-01

    This report describes an architecture and implementation for doing group key management over a data communications network. The architecture describes a protocol for establishing a shared encryption key among an authenticated and authorized collection of network entities. Group access requires one or more authorization certificates. The implementation includes a simple public key and certificate infrastructure. Multicast is used for some of the key management messages. An application programming interface multiplexes key management and user application messages. An implementation using the new IP security protocols is postulated. The architecture is compared with other group key management proposals, and the performance and the limitations of the implementation are described.

  18. EARNED VALUE MANAGEMENT SYSTEM

    Broader source: Energy.gov (indexed) [DOE]

    ... the costs associated with the authorized resources identified to accomplish the work and invalidates management's forecasting of future resource requirements and their costs. ...

  19. ORISE: Peer Review Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Management Man participating in a peer review The Oak Ridge Institute for Science and Education (ORISE) ensures that scientific reviews are conducted in a professional manner and...

  20. Acquisition Career Management Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2015-05-14

    The order sets forth requirements and responsibilities for the Department of Energy (DOE) Acquisition Career Management Program. Supersedes DOE O 361.1B.

  1. Environmental Management System

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    of those activities, prioritizing improvements, and measuring results. May 30, 2012 The continuous improvement cycle Our Environmental Management System encourages continuous...

  2. Chemical Industry Corrosion Management

    SciTech Connect (OSTI)

    2003-02-01

    Improved Corrosion Management Could Provide Significant Cost and Energy Savings for the Chemical Industry. In the chemical industry, corrosion is often responsible for significant shutdown and maintenance costs.

  3. Parallel integrated thermal management

    DOE Patents [OSTI]

    Bennion, Kevin; Thornton, Matthew

    2014-08-19

    Embodiments discussed herein are directed to managing the heat content of two vehicle subsystems through a single coolant loop having parallel branches for each subsystem.

  4. Management Control Program

    Broader source: Directives, Delegations, and Requirements [Office of Management (MA)]

    2002-04-18

    To establish requirements and responsibilities for the Department of Energy Management Control Program. Cancels DOE O 413.1. Canceled by DOE O 413.1B.

  5. Information Exchange management site

    Energy Science and Technology Software Center (OSTI)

    2012-08-01

    Django site used to manage the approved information exchanges (content models) after creation and public comment at https://github.com/usgin-models.

  6. Risk Management RM

    Office of Environmental Management (EM)

    Modules that address key functional areas of project management, engineering and design, safety, environment, security, and quality assurance, grouped by each specific CD phase. ...

  7. Efficient Water Use & Management

    Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)

    Water Use Goal 4: Efficient Water Use & Management Aware of the arid climate of northern New Mexico, water reduction and conservation remains a primary concern at LANL. Energy ...

  8. Dietary turmeric modulates DMBA-induced p21{sup ras}, MAP kinases and AP-1/NF-{kappa}B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

    SciTech Connect (OSTI)

    Garg, Rachana; Ingle, Arvind; Maru, Girish

    2008-11-01

    The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12 weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished the DMBA-induced mRNA expression of proto-oncogenes (c-jun, c-fos) and NF-{kappa}B, leading to decreased protein levels and in further attenuation of DMBA-induced AP-1/NF-{kappa}B DNA-binding in the buccal pouch nuclear extracts. Besides, buccal pouch of hamsters receiving turmeric diet showed significant alterations in DMBA-induced effects: (a) decrease in cell proliferation (diminished PCNA and Bcl2 expression), (b) enhanced apoptosis (increased expression of Bax, caspase-3 and apoptotic index), (c) decrease in inflammation (levels of Cox-2, the downstream target of AP-1/NF-{kappa}B, and PGE2) and (d) aberrant expression of differentiation markers, the cytokeratins (1, 5, 8, and 18). Together, the protective effects of dietary turmeric converge on augmenting apoptosis of the initiated cells and decreasing cell proliferation in DMBA-treated animals, which in turn, is reflected in decreased tumor burden, multiplicity and enhanced latency period. Some of these biomarkers are likely to be helpful in monitoring clinical trials and evaluating drug effect measurements.

  9. Energy Management Webinar Series

    Broader source: Energy.gov [DOE]

    Boost your knowledge on how to implement an energy management system through this four-part webinar series from the Superior Energy Performance program. Each webinar introduces various elements of the ISO 50001 energy management standard—based on the Plan-Do-Check-Act approach—and the associated steps of DOE's eGuide for ISO 50001 software tool.

  10. Tribal Financial Management Symposium

    Broader source: Energy.gov [DOE]

    The 3rd Annual Tribal Financial Management Symposium is a two-day event will focus on one of the most important aspects of a tribe's financial management. Attendees will learn about the state of the economy, building a financial constitution, both long- and short-term investments, alternative investment options, and much more.